LOCUS       DAOQHY010000001        54943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_188_length_54943_cov_5.846451, whole genome shotgun sequence.
ACCESSION   DAOQHY010000001 DAOQHY010000000
VERSION     DAOQHY010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 54943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54943
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188_length_54943_cov_5.846451"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="PLL28_00005"
     CDS             complement(<1..717)
                     /locus_tag="PLL28_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNF67719.1"
                     /translation="MRLIQNLPNLMTLFNLFLGCMGVVYLYNDHMVIMSKQDDMLIDM
                     GLIHLTCYCVMLAGVVDFFDGFVARMLKVQSELGKQLDSLADMVTFGLVPGLVMYELL
                     ARAYYAHYNSFEYPILYYAAGFSVTLFAALRLAKFNIDSRQTTEFRGLPSPAMAMVVV
                     SLPLILLRNEMGMTATLTNHWVLLGTIVLLSYLMVSDIPMLSLKVKNFKLQDNQWVFG
                     LLLIAAIILFFGLVIFHILFA"
     gene            complement(786..2081)
                     /locus_tag="PLL28_00010"
     CDS             complement(786..2081)
                     /locus_tag="PLL28_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HNF67720.1"
                     /translation="MKYLPIDSQLFVQNRKQYTTHLKPNSIAIFNSNDEMPFNADQTH
                     PWKQNSDIFYLSGIDQEETILLIYPESPNPKYREALFLKKTNEHIAIWEGHKYTEAEA
                     RAASGIETIHWVEDFNTVFPMLMNHAEHVYLNTNENDRASIKVPTRDTRFIEETKLRF
                     PMHKYERSAPIMAKLRTVKSIWERDLMQIACDITNKAYRRVLNFVKPGVMEFEIEAEI
                     QHEFLRNRGTRPAYGSIIASGPSACVLHYVDNNLRCNDGDLLLMDFGVEYANYCADLT
                     RTIPVSGKFTQRQKDVYNAVLRVQKKAIDMMHIGMILKDFNAEVGKVMESELLGLGLI
                     SKQDIADQNPEWPAFKKYFMHGTGHFLGLDVHDIGDHYQPIPAGAVLTCEPGIYIREE
                     GIGIRIENDIMLMPDGSKYDFMKDTPSEVEEIETIMNSK"
     gene            2318..3241
                     /locus_tag="PLL28_00015"
     CDS             2318..3241
                     /locus_tag="PLL28_00015"
                     /inference="COORDINATES: protein motif:HMM:NF036911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamine glutaminase family protein"
                     /protein_id="HNF67721.1"
                     /translation="MKLLKCLLPLSLLFLITQSCQKTDQLIPDAAIETSNDAFRLMTP
                     AYYRESENGSSIQVRFFESPRIFDLPKNSAEYANIKSLIDISRDAKQPMRVYFGDITM
                     PYISITGAQKATAEEIRLNEQKFVITPDNAKLKKVLPGMAVMNGIFDYCAAQGCTTGT
                     ATIDYCIPFQYVVDGCYARAHKMRQIMNEEYNYEPQKVFSYEGPSGSLAVDAGDCCVY
                     WWYHVAPLVTVKTATGTAQYVIDPSMFDEPVSIATWTAAQENLGCTPFADFGYTEITA
                     GKIYAPGGSTDNSYISTNWTLQFYADLVTCP"
     gene            complement(3315..3953)
                     /locus_tag="PLL28_00020"
     CDS             complement(3315..3953)
                     /locus_tag="PLL28_00020"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNF67722.1"
                     /translation="MKKILTIIFCSLAVAASAQQFRYGANVAPTVSFWLVEGDNYVPD
                     GMKVGFQVGGTIDLTLGDAERFALQSGLNFTSAPGAFKDNPEGLASNGKAWDYSITTL
                     DLPILLRLRSDELGKAVLFAQYGLTLGFTLTEKITVNDGKNGGSSFDYEGSNTSLSMG
                     AGAEFGLDNDKTLVFTGFFQNGIKNMVVNTNNPENDSRYFPQQIGLRATMLF"
     gene            complement(3990..4655)
                     /locus_tag="PLL28_00025"
     CDS             complement(3990..4655)
                     /locus_tag="PLL28_00025"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNF67723.1"
                     /translation="MRKFTFLLLMLVAFSSRAQQFRYGFSIAPGSNKLVLPADMYDPA
                     VSFSGFQYGLLLDQTFVKGDIIGVSLGLNLNYSQSGMTSTDNNGAGDDKEWAVRARYL
                     EIPLTLKLRTPQFGSFTIYGEGGATYGKCLRAVGDYTLNGLTLDSDIDYLEKDNANGI
                     NYLDKNAGLQFGLGAEVEVAEGASIIIGFFYQKGIMNVYEDNNVGSDILLNQTGIRIA
                     GLF"
     gene            5231..5629
                     /gene="rpsL"
                     /locus_tag="PLL28_00030"
     CDS             5231..5629
                     /gene="rpsL"
                     /locus_tag="PLL28_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNF67724.1"
                     /translation="MPTIQQLVRKGRDVMKAPSKSRALDACPQKRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNQTEIIAYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHIVRGAL
                     DTAGVNNRKKSRSKYGTKRPKAGAAPAGKK"
     gene            5661..6128
                     /gene="rpsG"
                     /locus_tag="PLL28_00035"
     CDS             5661..6128
                     /gene="rpsG"
                     /locus_tag="PLL28_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNF67725.1"
                     /translation="MRKLKPKKRILPADPQFNDATVTRFVNMMMWQGKKSTAYTCFYD
                     ALALVEKQTGESGYEVWKKALSNVTPGVEVKSRRIGGANFQIPTEIRPDRKVSLSMKW
                     LIMFSRKRSGRSMAEKLAAEIVAASKGEGATVKKKEDVHKMAEANKAFAHFKS"
     gene            6147..8255
                     /gene="fusA"
                     /locus_tag="PLL28_00040"
     CDS             6147..8255
                     /gene="fusA"
                     /locus_tag="PLL28_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNF67726.1"
                     /translation="MARDLKYTRNIGIAAHIDAGKTTTTERILFYTGMTHKIGEVHDG
                     AATMDWMEQEKERGITITSAATTTAWKYNKEEYKVNIIDTPGHVDFTVEVERSLRVLD
                     GVVALFCAVGGVEPQSETVWRQANRYRVPRIGFVNKMDRSGADFLDVVRQVKEVLGAN
                     PVPLVLPIGAEETFRGVVDLIANRAIIWDEETQGMTYKEVPIPDDMKDEVIEYRGKLV
                     EAVAEYDDALLEKFFDNPDTITEAELIEAIRKATIDIAIIPMLCGSSFKNKGVQFMLD
                     AVCRYLPSPLDIDAVKGTNPDTEEELLRRPDAKEPFTALAFKIMTDPFVGRLAFFRVY
                     SGRLDAGSYVLNVRTGKKERISRILQMHANKQNPIDFIEAGDIGAAVGFKDIRTGDTL
                     CDEDHPIALESIKFPDPVIGLAIEPKTTADADKLGMALAKLAEEDPTFKVFFDDETGQ
                     TVVRGMGELHLEIIVDRLKREFKLEVNQGAPEVAYKEAFTKVHKHRETYKKQTGGRGK
                     FADIQFEIGPADEGVIGLQFVNDIFGGSIPREFVPAVEKGFKEAMSTGVLAGYNVDSM
                     KVRLFDGSFHAVDSDQFSFEMCARMAFREAGRQAGPQLLEPIMKLEVITPEDHMGDVI
                     GDLNRRRGMIEGFDNKNNATVIKAKVPLSEMFGYVTQLRTITSGRATSILEFSHFAQA
                     PNNIAEEVIAKSKGKVSVDN"
     gene            8276..8581
                     /gene="rpsJ"
                     /locus_tag="PLL28_00045"
     CDS             8276..8581
                     /gene="rpsJ"
                     /locus_tag="PLL28_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNF67727.1"
                     /translation="MTYKIRMKLKSFDHTLVDKTAEKIVKTVRNTGAVVTGPIPLPTQ
                     KKIYTVLRSPHVNKKSREQFQLCTYKRLLDIYSATNKTVDALQKLELPSGVEVEIKI"
     gene            8594..9211
                     /gene="rplC"
                     /locus_tag="PLL28_00050"
     CDS             8594..9211
                     /gene="rplC"
                     /locus_tag="PLL28_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNF67728.1"
                     /translation="MKGIIGKKVGMTHYFGEDGKMITCTVVEAGPCVVLQKKTDDTDG
                     YNAIQVGFGEKKEKNVSAGMKGHFKKANAQASTKIREFRNFTEDKNVGDTITVDIFEE
                     GETVHVVGTSKGKGFQGVVKRHNFNGVGMRTHGQHDRHRAPGSIGSSSFPSRVFKGMR
                     MAGRMGNDTVKMRNIKVAKIISDKNLILLNGSVPGSKGSYVIIEK"
     gene            9213..9836
                     /gene="rplD"
                     /locus_tag="PLL28_00055"
     CDS             9213..9836
                     /gene="rplD"
                     /locus_tag="PLL28_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNF67729.1"
                     /translation="MKIDIVKISGEKTGRQVELNDAIFGIEPNDHAIYLSVKQYLANQ
                     RQGTHKAKERSEVAGSTRKLHRQKGTGGSRKGDIKNPIFRTGGTIFGPRPRNYSFKLN
                     RKVKELARRSALAYKAQQNGIVVVEDFNFDAPKTKMFAGIMKSLGISDKKSLVVAGEN
                     ENVMLSMRNLPKVSSTRAEDLSTYSVMNAQCLVLTESAVKQINQSAE"
     gene            9857..10153
                     /gene="rplW"
                     /locus_tag="PLL28_00060"
     CDS             9857..10153
                     /gene="rplW"
                     /locus_tag="PLL28_00060"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNF67730.1"
                     /translation="MAKRILIKPLITEKTTKLNEEHGAYGFVVGLDANKIEIKKAVEK
                     HYGVKVESVRTVMDPGKFGWNRTPFGISQGTKGRMKKAYVTLKKGETIDFFSTI"
     gene            10162..10986
                     /gene="rplB"
                     /locus_tag="PLL28_00065"
     CDS             10162..10986
                     /gene="rplB"
                     /locus_tag="PLL28_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNF67731.1"
                     /translation="MALKKYNPVTPGTRFRIGNAYSEVTTDTPEKSLLAPHKKSGGRN
                     SSGHLTMRYIGGGHKRKFRVIDFKRDKHEINAVVKTVEYDPNRTAFIALLVYADGEKR
                     YILAPNGIQVGQTVQSGLNAAPELGNALMLRNIPLGTIIHNIELYPGRGGMIARSAGS
                     WAQLSAKEGKYVIIRMPSGETRMILDTCFATVGTVSNPDHNLQQLGKAGRKRWLGRRP
                     RNRGVAMNPVDHPMGGGEGRSSGGHPRSRNGQIAKGLKTRQPKKTTSKFIISRRKK"
     gene            10998..11261
                     /gene="rpsS"
                     /locus_tag="PLL28_00070"
     CDS             10998..11261
                     /gene="rpsS"
                     /locus_tag="PLL28_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNF67732.1"
                     /translation="MARSIKKGPYVDHNLAGKVAQMNESGKRTVMKTWSRRSTILPEM
                     VGHTFAVHNGNKFIPVYVTENMVGHKLGEFSPTRNFKSHAGNR"
     gene            11267..11650
                     /gene="rplV"
                     /locus_tag="PLL28_00075"
     CDS             11267..11650
                     /gene="rplV"
                     /locus_tag="PLL28_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNF67733.1"
                     /translation="MEAVAKLINNPRTNNNSGSPRKARYVVDMIRGLDIDKALSVLKY
                     TNRRNVTAVEQTLRSAIKNWESKYEMDAADSNLYVKEAFVDGGKTMRRFLPAPHGRAY
                     RMRKRTNHLTIVVASRNANNQEKNA"
     gene            11668..12375
                     /gene="rpsC"
                     /locus_tag="PLL28_00080"
     CDS             11668..12375
                     /gene="rpsC"
                     /locus_tag="PLL28_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNF67734.1"
                     /translation="MGQKCNPIGNRLGIIRGWDSNWYGGKTMAEKLVEDEKIRQYLNA
                     RISKGGISRIVIERTLNRITVTIHTSRPGIIIGKGGGEVDKLKEELKKITEKEVQINI
                     IEIRRPELDANIVGETIARQLEARINFRRAIKMAVASTMRMGAEGVKIRVSGRLNGAE
                     MARTEEYKQGRVPLHTFRADIDYSWKEAHTVYGKIGVRVWICKGEVLGKRDLSPNVGM
                     SQEKERSKQGRRRPNKR"
     gene            12390..12809
                     /gene="rplP"
                     /locus_tag="PLL28_00085"
     CDS             12390..12809
                     /gene="rplP"
                     /locus_tag="PLL28_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNF67735.1"
                     /translation="MLAPKKVKHRKTFKGKMKGDAKKGSQLSTGSFGLKTLESGWITD
                     RQIEAARVALTRHLKREGQVWINIFPDKPITKKPAEVRMGKGKGNPELWVAVVKPGRI
                     MFEAEGVPLDTAREAMRLAAGKLPVKTKFVVRRDYVA"
     gene            12824..13033
                     /gene="rpmC"
                     /locus_tag="PLL28_00090"
     CDS             12824..13033
                     /gene="rpmC"
                     /locus_tag="PLL28_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNF67736.1"
                     /translation="MAKLKREDLNQLSNEDLQQRLHDDSQHLTKLQFTHAVNPIDNPS
                     QLRSIRREIARIKTEIRKREIASAK"
     gene            13045..13308
                     /gene="rpsQ"
                     /locus_tag="PLL28_00095"
     CDS             13045..13308
                     /gene="rpsQ"
                     /locus_tag="PLL28_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNF67737.1"
                     /translation="MEQSTRKLRRTIIGVVTSDKMDKSITVTVERKVMHPKYGKFLKK
                     SKKYHAHDEANDAKSGDLVKIMETRPLSKLKRWRLVDIVERAK"
     gene            13310..13678
                     /gene="rplN"
                     /locus_tag="PLL28_00100"
     CDS             13310..13678
                     /gene="rplN"
                     /locus_tag="PLL28_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNF67738.1"
                     /translation="MIQQETRLKVADNSGAREVLCIRVLGSTRRRYASLGDKIIVTVK
                     DALPGGGIKKGTVSTAVVVRTHKEVRRKDGSYIRFDDNACVLLNASDEPRGTRIFGPV
                     ARELRDKQFMKIVSLAPEVL"
     gene            13681..14028
                     /gene="rplX"
                     /locus_tag="PLL28_00105"
     CDS             13681..14028
                     /gene="rplX"
                     /locus_tag="PLL28_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNF67739.1"
                     /translation="MKETNYSNRFQPKYHVKKNDTVVVIAGADKGKKGRVIEVDYAKN
                     RVMVEGVNIVSKHTRPTQQYPNGGIIKKEAPIHISNVMPVDPKSGKGTRVGRKEENGK
                     SVRYSKKSGEIIK"
     gene            14028..14582
                     /gene="rplE"
                     /locus_tag="PLL28_00110"
     CDS             14028..14582
                     /gene="rplE"
                     /locus_tag="PLL28_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNF67740.1"
                     /translation="METKSPRLKKQYHETVVKDLQEKFQYKSSMQVPKLLKIAINQGL
                     GAATQDKKVVDVALDELTRITGQHAVSTISKKAISNFKLRENMPIGARVTLRGNNMYE
                     FLDRLISVALPRVRDFKGVNNKSFDGRGNFTLGITEQIIFPEIDIDKVTKITGMDITF
                     VTTATTDAEAYELLKGLGMPFRKA"
     gene            14592..14861
                     /gene="rpsN"
                     /locus_tag="PLL28_00115"
     CDS             14592..14861
                     /gene="rpsN"
                     /locus_tag="PLL28_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HNF67741.1"
                     /translation="MSKKSVIARNNKREQMIIKFAERRKQLKAEGNWEALDKLPRNAS
                     PIRYKNRCKLTGRPRGYVGDLGISRIKLRDLALYGKIPGMTKASW"
     gene            14887..15288
                     /gene="rpsH"
                     /locus_tag="PLL28_00120"
     CDS             14887..15288
                     /gene="rpsH"
                     /locus_tag="PLL28_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNF67742.1"
                     /translation="MKVTDPIADFLTRIRNAQKANHRIVEIPSSQMKQRLTEILYENG
                     FIYKYKFEQEGPQGTIKIALKYDPVTKSPAIKGIERVSSPGLRRYTGVDSLPRVMSGL
                     GLAIMSTSKGVMTEKEARRQNVGGEVLCYVY"
     gene            15311..15865
                     /gene="rplF"
                     /locus_tag="PLL28_00125"
     CDS             15311..15865
                     /gene="rplF"
                     /locus_tag="PLL28_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017722933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNF67743.1"
                     /translation="MSRIGRLPIQLPAGVTLSVAGNNMVTIKGKRGELTLQVDPDITV
                     KTEDNAIKLERPSEQKRHRAMHGLYRALIANMVKGVSDGFKKELELVGVGYRAANTGQ
                     ILEISVGYSHPIMMMIPKEVKLSTVSEKGKNPTIILESNDKQLLGAIAAKIRSFRKPE
                     PYKGKGIKYSDEVLRRKAGKTAGK"
     gene            15877..16233
                     /gene="rplR"
                     /locus_tag="PLL28_00130"
     CDS             15877..16233
                     /gene="rplR"
                     /locus_tag="PLL28_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNF67744.1"
                     /translation="MLSKKSIRRQKIRYRIRKKIAGTAECPRLAVFRSNKEIYVQLID
                     DVAGHTLCAASSNDKQGKAYTGTKVEKAAAVGKQIAAQAQAKGITTVIFDRGGYLYHG
                     RVKSLADGAREGGLQF"
     gene            16249..16770
                     /gene="rpsE"
                     /locus_tag="PLL28_00135"
     CDS             16249..16770
                     /gene="rpsE"
                     /locus_tag="PLL28_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNF67745.1"
                     /translation="MAVNKDIQRVRASELELKEKIVGISRVAKVTKGGRTFSFSALVV
                     VGDGNGTVGHGLGKAREVTEAIQKGIDDAKKNLIKVPVFNGTIPHEQHGKFGAGKVMI
                     KPAAHGTGVIAGGAMRAVLESAGVTDVLAKSLGSSNPHNVVKATLDALAHLRSPLAVA
                     QHRNVSVKKVFNS"
     gene            16794..16973
                     /gene="rpmD"
                     /locus_tag="PLL28_00140"
     CDS             16794..16973
                     /gene="rpmD"
                     /locus_tag="PLL28_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNF67746.1"
                     /translation="MAKIKITSVKSTTDRPKNQKATMKALGLRRMHQTVEVEATPQII
                     GMVRVVQHLVKVENV"
     gene            16975..17427
                     /gene="rplO"
                     /locus_tag="PLL28_00145"
     CDS             16975..17427
                     /gene="rplO"
                     /locus_tag="PLL28_00145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNF67747.1"
                     /translation="MDLSNLKPASGSIKKRHRIGRGQGSGWGETSKRGFKGAGSRSGT
                     STKRGFEGGQMPLQRRLPKRGFNNKNFETKYVTLNLDQIQEMVDKYEITKLDQDMLVA
                     NRIIKGDDKLKVLGRGELKAKVEIWAHACSETAQKAIEAAGGTITMIG"
     gene            17431..18786
                     /gene="secY"
                     /locus_tag="PLL28_00150"
     CDS             17431..18786
                     /gene="secY"
                     /locus_tag="PLL28_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNF67748.1"
                     /translation="MKQFIQTLKNIWSIEDLRKRIIYSLGLILLYRFGSYVMLPGIDA
                     GIYNQAANVDSQSGLLGLINLFAGGGFSRASIFALGIMPYISASIAIQLLGIAVPYFA
                     RLQKEGESGRRKMNQYTRYLTVIVTALQASGYYAYLNTMSKNADFIVAPQGLFWFITV
                     VILTAGTLFTMWMGERITDKGIGNGISMIIMVGIIAGLPAALVNEITHRGAASGGGFI
                     PFLLEIAFLIVVIMGCILLVQGTRRIPVQYAKRVVGSRQYGGVRQYIPLKINAAGVMP
                     IIFAQALMFIPGLFAQINEDWQSSPVLVMLNDFQSLGYNLIYGFLIVLFTYFYTAIII
                     NPSQIADDLKKNGGFIPGIKPGKKTSEFIDNVLSRITLPGSIFLALVAISPVLTRAFH
                     VDPSFARFFGGTSLLIMVGVILDTLQQIESHLLMRHYDGLMKSGRIKGRTNAPVSIQQ
                     G"
     gene            18783..19595
                     /gene="map"
                     /locus_tag="PLL28_00155"
     CDS             18783..19595
                     /gene="map"
                     /locus_tag="PLL28_00155"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNF67749.1"
                     /translation="MIYYKTTDEITLIRKSSLLVSETLAMIAGIIKPGVTTLWLDQQA
                     EAFIRDNGGKPSFKGYKGYQHTLCVSVNDQVVHGIPSAREIKNGDVVSVDCGAFLNGF
                     HGDSAFTFLVGEVSREAVNLARVTRACVYKGIEFAVAGNRVGDVSHAIQLYAEHVNGY
                     GVVRELVGHGIGKNLHEEPEVPNFGAKGKGPKMQEGMVIAIEPMINLGSRNVVQENDG
                     WTIRTRDGKVSCHYEHTVAVMKGKADVLSSFEIIDEAMAKNSNLYTEKEIVG"
     gene            19595..19813
                     /gene="infA"
                     /locus_tag="PLL28_00160"
     CDS             19595..19813
                     /gene="infA"
                     /locus_tag="PLL28_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNF67750.1"
                     /translation="MSKQDLIKQDGTVTEALSNANFRVKLENEHEILATISGKMRMNY
                     IRILPGDRVTVEMSPYDLTRGRIIYRYK"
     gene            19823..19939
                     /gene="ykgO"
                     /locus_tag="PLL28_00165"
     CDS             19823..19939
                     /gene="ykgO"
                     /locus_tag="PLL28_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HNF67751.1"
                     /translation="MKVRPSIKKRSADCKIVRRKGKLYVINKKNPKFKQRQG"
     gene            19963..20343
                     /gene="rpsM"
                     /locus_tag="PLL28_00170"
     CDS             19963..20343
                     /gene="rpsM"
                     /locus_tag="PLL28_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNF67752.1"
                     /translation="MARIAGIDLPKNKRGVIGLTYIYGIGDTTAAQILAAAGVDESKK
                     VKDWNDDEINAIRSYINNELKVEGALRSDMQLSIKRLMDIGCYRGIRHRKGLPVRGQR
                     TKSNARTRKGRKKTVANKKKAPKG"
     gene            20386..20784
                     /gene="rpsK"
                     /locus_tag="PLL28_00175"
     CDS             20386..20784
                     /gene="rpsK"
                     /locus_tag="PLL28_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004641037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNF67753.1"
                     /translation="MAKTTSAKKTTKKRVVKVDAEGQAHILASFNNLIITFTNLSGQT
                     ISWASAGKAGFRGSKKNTPYAAQVACTDAAKVAYDAGLRRVEVFVKGPGSGRESAIRT
                     IVANGIDVSVIRDLTPMPHNGCRPPKKRRV"
     gene            20811..21416
                     /gene="rpsD"
                     /locus_tag="PLL28_00180"
     CDS             20811..21416
                     /gene="rpsD"
                     /locus_tag="PLL28_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNF67754.1"
                     /translation="MARYTGPTTKKSRSFGESIFGYDKAFEKRKYAPGQHGLSRKKKS
                     ASEYGVQLKEKQKAKYTYGLLERQFRKTFEAATRRHGVTGEALLQLLEARLDNTVFRL
                     GIAPTRRGARQLVNHKHIEVNGQICSSPSAILRPGDVITIREKSKDHEWILAAANNTG
                     KRYSWLEWNPTQLKGVFVNYPERDQIPENIREQLIVELYSK"
     gene            21438..22436
                     /locus_tag="PLL28_00185"
     CDS             21438..22436
                     /locus_tag="PLL28_00185"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNF67755.1"
                     /translation="MALLSFQRPDKITLQKATDFEGTFEFKPLEPGFGVTIGNALRRV
                     LLSSLEGFAISSVKISGVDHEFATINGVMEDITEIVLNLKQVRLKRELGGDENAVEKI
                     FISLKDKEEFKAGDIEKHTNSFRVTNPDLLICRMEPFVKLDIELTIQKGRGYLPAEEN
                     KNVDRTIGVIPIDAIYTPIKNVRYTIENTRVEQKTDYEKLLIEIKTDGTIHPEDAIKE
                     ASRILIQHLMLITDEHITFNTVGEKETNVVDEHTLHIRKLLKTPLEDLDLSVRAYNCL
                     KAAKINSLAELVRYDTNELLKFRNFGKKSLVEIEAMVLDKGLQFGMDLAKYNLDED"
     gene            22474..22974
                     /gene="rplQ"
                     /locus_tag="PLL28_00190"
     CDS             22474..22974
                     /gene="rplQ"
                     /locus_tag="PLL28_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNF67756.1"
                     /translation="MRHGNKINHLGRTTAHRKSMLSNMGTSLILHKRIKTTLAKAKEL
                     RVYIEPIINRGKEDTTHNRRMVFSELQSKEAVQELFTNISGKIAERNGGYTRILKIGG
                     NRVGDNAETCYIELVDYNENMLKTAKAGGAKKRSRRGSGKTEKKAVEPETKAQDAAEG
                     TSEEQA"
     gene            complement(23026..23892)
                     /locus_tag="PLL28_00195"
     CDS             complement(23026..23892)
                     /locus_tag="PLL28_00195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF67757.1"
                     /translation="MTLTAYATLAAAVIPMVAPADVVYTDVDPDVLVNEPGTGISLDL
                     NGDGTNDFNFFFSSANSAVFTYFGGLNWYIINGIFAAPQNDNAIAAFTGSGGAYAYPY
                     AIGSGINIGPYAGQFLTNPYQTLAYQFYALIGSYLYYPIFAGGDWVFGQEDKFVGLQL
                     AVGDSTYYGWIRLTTDPSNRAFTVKDFAWENNANTSIETFLQPTAITNPELLEVEMYA
                     SGNNIIIHLNEDPKNAYLLNVYNLSGQLILQDNILQKDSRIMVDAPQGMYIVELTGNN
                     QKAGKKLFIAGT"
     gene            complement(23981..24907)
                     /locus_tag="PLL28_00200"
     CDS             complement(23981..24907)
                     /locus_tag="PLL28_00200"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF67758.1"
                     /translation="MKKSFTTGLSRKNLAAYSSMAGTFLLLHTSAQGQVGYTDLDPDE
                     TLNPLDVYDLDMDNNGDIDFTFNFTTFTLPDFFYTVAYHSVYFDGLFNVMKIYPTPGN
                     GVVAYPLSTTGGATLAYAEALNAGADIDGGQAFYTNSAIYMGAFLSVKDYPGVGSNYQ
                     FYTFGAWPGKTGKFVGVRFEGDGNEYYGWVRVSVNNDDLEITIDDYAYLSTPETAIEA
                     GQLPTAIHNIDPSQLNVYANGNIINIGVNELNTTSADVNVYDIQGRVVYSNGLNMSGM
                     QITLDAVATGTYTVKITTAEGANYAKRVLINQ"
     gene            complement(25032..25883)
                     /locus_tag="PLL28_00205"
     CDS             complement(25032..25883)
                     /locus_tag="PLL28_00205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF67759.1"
                     /translation="MKKLYNSKNAGKLAAYSAMAGAFVATTSEANATVVYTDIDDVTV
                     ELGETYALDLDNNGYSDFLMAAVSNSAGNWTWAYMIGNLSAYGYGGGSNMVIGYTGPI
                     LSYGSALNDGDLIGPDASFVSNTYNMLWLASIYSGVTYGPFANTEDKYIGVQFDIDGS
                     LHYGWARLDVSVGPVSFTIKDYAYDDAEGTDISAGEKDVAINNLTEAQVAAYSYGNTI
                     NVVIKDSEAGVDNVNVFDIDGKLVYSARLTGSNMAISLDNAATGLYTLQLTGANTSFS
                     KSLYIQN"
     gene            complement(26001..26885)
                     /locus_tag="PLL28_00210"
     CDS             complement(26001..26885)
                     /locus_tag="PLL28_00210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF67760.1"
                     /translation="MKKDFTSTKKLLQYAASATAFLAINQAGATVVYTDLDPDLAIGG
                     EGAEASIDINEDGTDDFTFSIYNFSGSGTYSGFSFGYVVNIALGSALNGNEFLGSIVS
                     YSGYSGVYAPILPAGTLVGSDDNFANGAMSMAVSASVTVLGFPYYTYVGGNWLDTDMA
                     FMGFRINIDKDRYYGWMRVSVSDDATMITIHDYAYQSEPNTAIFVGQTATEIEENPLS
                     NAQIFSNGGNIVVDIPSDFTGTADLQLFDMNGKLVKTMSGLSGHNVLDCNDLASGNYA
                     VRLGNAELTVKKQLNLSI"
     gene            complement(26977..29535)
                     /locus_tag="PLL28_00215"
     CDS             complement(26977..29535)
                     /locus_tag="PLL28_00215"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNF67761.1"
                     /translation="MGKRIAKKVLLIGWDAADWKVINPLMDAGLMPTLEKLVNGGVIG
                     NHATLDPPLSPTLWTSIATGKRPYKHGIIGFTEPDPSGKGIRPVYITSRKVKAVWNIF
                     NQHNLKSHVIGWWPSHPAEPINGTMISNLYQRATKPINEPWPMLDGTVHPADKAELFK
                     KMRIHPAELTASHILPFIPDANKIDQEKDKRLGMLTKIIADCSTIHNAATYILENEEW
                     DFLAVYYDAIDHFCHGFMKFHPPQMPMVPDDLYNLYKDVVAGGYKYHDMMLERLLELA
                     GPDVTVMIISDHGFHPDHLRPIELPKEPAAPALEHSPYGIFVLNGPGIKKDERIYGAS
                     VLDVTPTLLTLFGLPVAADMDGNVLVNALETPVIPDKIDTWEDIPGDSGQHPADKQED
                     PYAAAEAMEQLIELGYIERPSENIEKAIKSTVSENNYYLARSYMNGRKYAEAVDILKK
                     LVDENPGTNRYAMRLAHAYQSMNKVEETREVLSKIKTENNGRESVSMKIMEGNLLLVE
                     NKPKMAMELFEAIEKEMPNAARVHMQLGRCYMALKRWKKAEGAFLKELEHDPGNASAH
                     HGLGITLLRRKHYEESIDCFLNAVGLTYFFPFAHYHLGEALYYLGEYERSAEAFEVCL
                     KMAPGINKARQLLSTIYSEHLKKPEAAVEVKSQIKDYIKGDIVVVSGLPRSGTSMMMQ
                     MLDKGGLEIFTDGLRTPDENNPKGYYEHELVKSLARNKTWMKDAMGKGVKVISHLLFE
                     LPANYRYRIIFMERDLDEVIKSQHHMLVRDGKAKEDALNLRIVNAFKQNLERVQTWVP
                     QQQNMEILYVSHRNLVNNPTEELKKINAFLGGTMNVDAMAAVVDKSLHREKSGK"
     gene            complement(29590..29799)
                     /locus_tag="PLL28_00220"
     CDS             complement(29590..29799)
                     /locus_tag="PLL28_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67762.1"
                     /translation="MEKKQGNKLRITTQDGKPFRIPVEGSLGLLALGYKGLIAWRQAK
                     WQFQKMKEAKQGGNTEQQQDEPIAD"
     gene            complement(29925..>32090)
                     /locus_tag="PLL28_00225"
     CDS             complement(29925..>32090)
                     /locus_tag="PLL28_00225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNF67763.1"
                     /translation="FYCTTGYAMPTVTGDPGGTFAAVPAGMVIDPVTGEIDLNASTPG
                     SYSVSYTAYGITGDCPSETAVDLVTIVVDPDASFGYSDINYCPTGTTTPTFITTAGGT
                     FSVSPAGLTVNPTTGVVNLTTGTIGTTYTITYTVGILCVSTYSVTITITDFDDPSVNY
                     PAPSYCPTGTATPTVATPGGTYTVSPAGLVVNPTTGVVDLTTGTVGTTYTITYTTPTG
                     PCSNSTTTTITIDPLDDPTFSYSAPSYCPTGTTLPTSITTPGGTFSIFPTTMGINSTT
                     GMLNLASGDIGTTYTITYTTPAGPCSNSSTETVTIDPLDDPGFTYDAASYCNYGTAIV
                     TSVETPGGNFTVSPATLSINALTGAIDLTTGIPGSFYTITYTTPAGPCSNSTSVVIEI
                     MPLTDAAFSYLLPAYCATGTTLPNMILNPGGTFSAMAGISINAATGELNLAACTPGGP
                     YEIYYTSPGCPEVDTVEVTIYPLPTPTIDLIDVICLEGDPTLIIGDPAGGTFSGEGVI
                     GDEFDPALAGDLGLYTVTYTYTDANGCTNNTTGAINVIQNDVDAGIDQYIPEYTTTVI
                     TATGGTTFLWEPPAGLSCTDCPAPVFDSLLSQTYMVTSWDAFGCIDSDMVTITVVPVF
                     DPVVFVPNTFTPNGDNINDYFFAYGTDLASIVSMNIYDRWGELIFTKENMIAGNPSAG
                     WDGTYNEEPVAQGVYAFMLIVQLEDGMKQHMQGNITLIR"
     assembly_gap    32091..32190
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<32191..32801)
                     /locus_tag="PLL28_00230"
     CDS             complement(<32191..32801)
                     /locus_tag="PLL28_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67764.1"
                     /translation="MRKLTAFLSVICLSSILPAQLVVTPDTTICAGGTATLAVISSPS
                     YGTSSYTFEVYPYSPETYAGTSPIQAGGGGLTDDSYSTAVPLGFTFCFLGNQYTQCYI
                     GSNGWVSFGGPGALVTTFTSAAIPSVAGTVPKNCIMGPWQDWHPGLCTPVGSCIKYQT
                     IGTAPNRKFVVSWDNVPMFSCTGLYGKFQIVLNETTNVIENHLT"
     gene            complement(32939..33012)
                     /locus_tag="PLL28_00235"
     tRNA            complement(32939..33012)
                     /locus_tag="PLL28_00235"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32976..32978),aa:Asn,seq:gtt)
     gene            33139..34059
                     /locus_tag="PLL28_00240"
     CDS             33139..34059
                     /locus_tag="PLL28_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNF67765.1"
                     /translation="MSLLVVGTVAFDAIETPFGKRDKVIGGSATYIGLSASYYYKPIH
                     IVSVVGSDFPKATLAAFNKKGITTDGVQVIKGQKSFFWSGKYHMDMNSRDTLRTDLNV
                     LADFNPVLPEKLRKPNMLMLGNIDPNLQIRVIEQLKSRPKLIAMDTMNFWMDIARPAL
                     DKVLKMVDVLIINDEEARQLTGEYSVVKAAKKIMKNGPKTLIIKKGEHGALLFSGSEV
                     FFAPALPLEEVFDPTGAGDTFAGGFMGYLSQSKTLSFTNMKGAIVYGSAMASFCVEEF
                     GPEKLLALKKPEIAKRVKMFSTLSKFSIKG"
     gene            complement(34049..34294)
                     /locus_tag="PLL28_00245"
     CDS             complement(34049..34294)
                     /locus_tag="PLL28_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HNF67766.1"
                     /translation="MDVNTLPAYNKFQLALRNGQDKEEIWIAYKGLIYDVTPSRLWRN
                     GKHYEHFAGQDLTDELKDAPHTEITLQKFNIIGRLIP"
     gene            complement(34285..34986)
                     /locus_tag="PLL28_00250"
     CDS             complement(34285..34986)
                     /locus_tag="PLL28_00250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF67767.1"
                     /translation="MEPDIKIEYLDWDSRFFGFKIGRLELQSTCIITEHQVGNWRSEG
                     YTCLYIDSATEVEISNRSSATLNPMSSRCLLQRETRAPQLNPEVKILTQVTDGIIELG
                     IHAGKHSRFMCDDHFNKKKTTALYAMWIRESLHGKMGDEIYFIGTEINPLGLIAFTNK
                     KSHMQIGLLAVAPGMRNQGIAKKLLTASDHFANAYQLPVMNLYTQGNNIAAQHLFAQD
                     GFTQISSINQFHLWM"
     gene            35045..35953
                     /locus_tag="PLL28_00255"
     CDS             35045..35953
                     /locus_tag="PLL28_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF67768.1"
                     /translation="MLTISNLTKSFGDHVAVDGISLSIAKGMFYGLLGPNGAGKTTTI
                     QMISSILPPDSGKISVDGLGVYEHASVKMHMGIVPQEIALYDELTALENLIFWGNLYA
                     VHGKTGKDRAAFLLDWVGLADRRHDIVKNYSGGMKRRVNIAAALMHDPGLIVMDEPTV
                     GIDPQSRNKIYELLDELHHQGKTILYTTHYMEEAERMCDKIGIIDKGKIIAEGSLDEL
                     KKAHAQEENIVIHFTGEAVSKLGNYVISFPEPQMMTVVTPQARKRLHDVVQLCTEGGI
                     DITGIDIHDAGLESIFLNLTGRQLRD"
     gene            35964..37175
                     /locus_tag="PLL28_00260"
     CDS             35964..37175
                     /locus_tag="PLL28_00260"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF67769.1"
                     /translation="MWTIAKKDIYIFFKDKRGLFLSLLLPIGLITMFAFAFGGIGQDD
                     EDPAPIHVLYVDADQTTSSKSLIAMLDTIPGIACLSTTAEDATANIKSGDEMAAVVIN
                     KGFEMALQTGEDLPVVLQYDQSRVMEIGVLQQLLTSRLSAMKGSVDAHRGINKIMQQS
                     FSNMPPNVQDSIRTSLEENLLQKENHEQLISMQEVVGDAESNWGLIQAVAGTAIMMLL
                     FSVSSIGKSMLDEKESGVLRRLLQTPMHPYGLLFGKMITALVIAVFQLTIMFLFSWLA
                     FGLDIFINIPALIIMILATSLACSAFGVLLASLVTTRKQADSLSTILILFMSAVGGSM
                     IPLFIMPVFMQNAAVVSVNYWSIQGFYDIFWRQAGLEAIVGNALVLTGISAGVLLISM
                     YFYKKNILRLI"
     gene            37201..38736
                     /locus_tag="PLL28_00265"
     CDS             37201..38736
                     /locus_tag="PLL28_00265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF67770.1"
                     /translation="MKRILLSCIIFSGFIASAQDALDPSFGGDGIVTLDFGGNDIAYA
                     VLIQPDGKILAAGQAGIGPALTTDIAIARYNTDGTLDPVWGGDGTVTLDLGVDYSDFS
                     FALARQPDSKIVVAGCGFFDSGYKFAVLRYRADGSLDPAFSDDGIFITDFGGMDDWAR
                     AVYVMPDGKIVASGYRNDGLKHNFAIIRLTPEGDLDPTFGTGGKVYTVFPVNRHDQSY
                     ALAVQPDGKMILAGYSYVGTTADYQFALARYNEDGSLDDTFGDAGLVLTPVPMIQGES
                     YAMELQPDGKILVSGFSYTDWKTDIAIIRFNSDGSVDTEFGTDGLVKTDIATDRDDHA
                     YAMYLLADGRFLVGGNRWNGYDWDMALLRYNADGSLDNTFSGNGMLTIAIGDDADCIQ
                     AIDVQSDGKIVVAGYSDNGPNNDFALVRFMQDGGSGVGISNQVVTANFSISPNPGYDN
                     ISINCTLSGIIDIVDMQGHVVERREVVSGDNTIDIHQLPTANYIIRWRNESAQCSLPF
                     IKK"
     gene            complement(38745..39212)
                     /locus_tag="PLL28_00270"
     CDS             complement(38745..39212)
                     /locus_tag="PLL28_00270"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNF67771.1"
                     /translation="MDIIINIIAFLAAFAFMEAFAWFMHKYIMHGVGWFLHRSHHEPR
                     HGRFELNDLYAIIFAAPAIYLMYIGSPAYNWIFWVGTGISAYGLCYFGFHDVLVHRRM
                     KHHMRPESKYMQRIIRAHKIHHKKLGRKDGEAFGFLFALPKYDVKKTNADNEE"
     gene            complement(39248..39967)
                     /locus_tag="PLL28_00275"
     CDS             complement(39248..39967)
                     /locus_tag="PLL28_00275"
                     /inference="COORDINATES: protein motif:HMM:NF039186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HNF67772.1"
                     /translation="MNQSYLYLLINTGTILFPFLFSFESRVRYVNWWKWLFPSMFITA
                     LVFLTWDQIFTVNGVWGFNDRYITGAHFMNLPIEEILFFFTVPYASIFIYAFTNTIWP
                     DTPTLDKYANKISYAMLTIALILLVANYNKAYTALNCGYAVVLLCIQLFLVKGTYMGK
                     FYRFYLWHLIPFFIVNGILTGWGIAEEVVWYNNHENLGIRMGTIPIEDSLYSFSLMLM
                     NIMLIEGFKSRSKKKNALIGA"
     gene            complement(39964..40809)
                     /locus_tag="PLL28_00280"
     CDS             complement(39964..40809)
                     /locus_tag="PLL28_00280"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HNF67773.1"
                     /translation="MLHLFHESSFTCSRKVTELYSTSFTLGIKALDRRYHDAIYAIYG
                     FVRYADEIVDTFHDWDKQTLLLRFKEDTWSALDEKISLNPILHAFQLTVHKYQIDRNL
                     IDAFLHSMEMDLHMSNYKQHHYEEYIYGSAEVVGLMCLKVFCENDETHYQKLIQPARF
                     LGAAFQKVNFLRDLQSDYKDRGRVYFPNVDFENFCERQKTQIEQEIANDFDEAYKGIK
                     QLPEGAKFGVYLAYKYYLELFKKIRSTEPEAVMQTRIRVSNPKKIGLWASLYLRSNVL
                     GIYAP"
     gene            complement(40864..42345)
                     /gene="crtI"
                     /locus_tag="PLL28_00285"
     CDS             complement(40864..42345)
                     /gene="crtI"
                     /locus_tag="PLL28_00285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HNF67774.1"
                     /translation="MAERKHIIVIGAGFSGISAACALAAKGYSVTILEKNESAGGRCS
                     VFSEQGFTFDMGPSWYWMPDVFEWFFNTYGSSVEQQYALKRLDPGYRIVFGPGDVMEI
                     PASISELKALFESIEPGSGIQLQKFLDDAAYKYEVGMKDLVFRPCNSIMEFAELRMVK
                     ALFTMQLFTSLRSEVYAKFKNERIRKILEFPVYFLGALPSNTPALYSILNYADLVLGT
                     WYPMGGMYKIIEGMQRVAEGLGVQFQFNSPVEKIIVEEGRSTGVKVHGDFIAASGIIA
                     AADYHHVEQKLLDQPYRNYDETYWDKKIFAPSAIIYYLGVKGRVKNLLHHNLFFDQSF
                     EQFADEIYTDPRWPEQPLFYVSAPSVTDPTVAPAGDENLFILVPVAAGLKDTAELREE
                     YYHKAMDRLEAHCGEEIKSRVVYKREFAPGNFVDRYNACKGNAYGLANTLMQTAIFKP
                     KMRNKRVRNLCYAGQLTVPGPGVPPSLISGYVAAQEMEKLVGH"
     gene            complement(42401..42703)
                     /locus_tag="PLL28_00290"
     CDS             complement(42401..42703)
                     /locus_tag="PLL28_00290"
                     /inference="COORDINATES: protein motif:HMM:NF025479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HNF67775.1"
                     /translation="MYLYNVTIKIEQSRADEWLTWMREKHIPDVMATGFFVEYRLCRL
                     VDDGDMEGMTFVVQYTLNNLDDFLTYKLTSGPSLQRDTQEKFGNDFFAFRTLMEII"
     gene            43485..44456
                     /locus_tag="PLL28_00295"
     CDS             43485..44456
                     /locus_tag="PLL28_00295"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNF67776.1"
                     /translation="MDLEVAFEVIYLKLLIPQLAMHTRQSTYIQRINDALRYIDLHID
                     AELSLDVVAKVAFYSPFHFHRLFKAVTGEALLVYITRRRIEHSGLMLMHRPSMSVSEI
                     AYAFGFSSVAVYSKTFKKVMGLSPTAFRDELNIKNSKIGQLDSKIGQDDLISEAYICN
                     INNLKKWLMMQAKISIKDTHDLHFAYLTQIGVDGIESTFQQVLKWAGPKAILSKDETF
                     ISRVFHDSFKVTDESKVRMSIGIASRHELPDDQHMSSGVISGGKTFIGRFEITLEEFE
                     PAWNSIFIEMHDRGYNKADGFPYELYHNNPANHPEKKYIVDLCIPIE"
     gene            complement(44463..46265)
                     /locus_tag="PLL28_00300"
     CDS             complement(44463..46265)
                     /locus_tag="PLL28_00300"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF67777.1"
                     /translation="MKKLVIICCFLSCYLTAGAQTDAALAQQYMSTGQYDKAADLYKN
                     LFNDNPNGYYKAYYNCLLVLQDYKEVEKIIDKQIKKNPDNLTYYVDYGNVLEKQGDKK
                     NATKQYESAIQKMTDSRPQVTQLANAFIGYDMPENAIRVYERARDIVSTYSFNYELAG
                     LYYRAGSFDQALDTYLDYYVEDPMGGSAKVTSAFSRILDEEKDHTLLQEKLFARIQNG
                     KYELQYTELLIWDYIQQKDFDGALIQAKALDKRNKENGERVYDLGETARAEESYDAAI
                     TAFEYIISKGKDSPYYFSAKNGILNCHKDKIFKLGQYTKSDIDALRNSYTAFLAEYDR
                     KDARAASVTDDLAKLEAFYAADFAKAISLAEPLVDWPAISAADRSRIKLDLGDFYLIS
                     GDVWEATLLYAQVDKAMKDEPLGEEARFKSAKLAYYRGDFSFAQGMLDVLKAATSELI
                     SNDAMQLSVFITDNLGLDSIAEPMMLYAKADLLLFQNNIQGALKVLDTLTTTYPAHQL
                     SDDVLFLKAKIAIKQQDYTTAVNYLEQIRQNYAYELLGDDAIYMLGDIYQYQFKDNEK
                     AQLCYEQIFMNFKDSLYSTEARKRYRQLRGDKIN"
     gene            complement(46335..47108)
                     /locus_tag="PLL28_00305"
     CDS             complement(46335..47108)
                     /locus_tag="PLL28_00305"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNF67778.1"
                     /translation="MEQSSPFLTDTFDEIVFHGRNKRYGSFVLRSTIRRNTFIGLYLM
                     TALTLLYTGIHLIDFSIDRSLPPSNFSNVEVTLSEPPPLFEVLPPAVPPAAAPQNLEK
                     VDEMEVKPDNEVSDIKPLTTDDKQIDSTSTGTATSGSTNNSAVTGDGNTIYSNVEVYP
                     QYPGGPKGLKKYLSDNVQYPEIAHKNNITGTVWVVFVVNQDGSVSDVKVEKGIGGGCD
                     QEAIRVVQGMRRWKPGMDGGKPVRVYQRIKIGFALDDNS"
     gene            complement(47546..47926)
                     /gene="gcvH"
                     /locus_tag="PLL28_00310"
     CDS             complement(47546..47926)
                     /gene="gcvH"
                     /locus_tag="PLL28_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNF67779.1"
                     /translation="MQFPAHLKYTKDHEWILIEGTTGTIGITEFAQSELGDIVYVELN
                     GLGDHMNQNDVFGTVEAVKTVSELFMPVSGEITEINAGLDSAPESVNKDPYGDGWMIK
                     VTMSDLSQVDGLMSAAEYQAHIGH"
     gene            complement(48043..>54943)
                     /gene="sprA"
                     /locus_tag="PLL28_00315"
     CDS             complement(48043..>54943)
                     /gene="sprA"
                     /locus_tag="PLL28_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HNF67780.1"
                     /translation="DASNILGGTGGKIELVPFDRECAIFDGCNVEIRPQGNVELTFGG
                     SFQNIQNPILTESQRRQGGFDFDMDINANVLGSIGNNLKLNFNYNTQATFDFENQIKL
                     EYKGKEDNIVQGIEAGNVSMPLTTQLIPGPTSLFGIKTELQFGRLTMTNILSQQKSQT
                     QSIQIEGGAQKREFEIFADQYDENRHFFMSHYFRKGYEGWLKTLPYVSSPINVTKIEV
                     WVTNTTGETQNTRDIVAFSDLGEPDSIYWGEIDPAGTVSSLTPGGLPSNNANNLYQKL
                     YGTAGTRDLNTVISTLTGPAFNMQAVRDYEKARMRKLDPTDFIFNPQLGFISLNTTLQ
                     PDDVLAVAYEYTYNGQTYQVGEFSSDLPPGVDTSNVLFMKMLKSTSAITQIPLWDLMM
                     KNVYSLGAFQVSSDGFRLDVVYQDPGGGMKRFIPANNSNVNSTPLIKLLGLDRLNNNN
                     DPQPDGIFDFVNGVTINTQNGRVYFPVLEPFGGYLRSDAVFGNDLEAEKYAYDELYNE
                     TKTIALQFPQYDRYVLSGEYKSSVSSEIYLGAFNLPKGSVKVTAGGQLLTENVDYTID
                     YSLGRLKIVNEAILNSGQQINVTFENNAFYGFQTKSLVGSRLDYWINDNFTLGATWMH
                     LSERPYTQKVNIGDDPISNSIYGFDINYTNDAPWLTRALDKLPFYGTKETSTISLTGE
                     VARFQPGHSKAIGKDEAGRIYIDDFEGSRSAYDLKFPYSSWVLASTPQGATDENNAIL
                     FPEAGLFDSLTYGMNRAKLAWYNIDPLFNEQNNPSTPDHLSDADLSNHYTIQIDEKEI
                     FPQADYETTVLTQLSTFDLAYYPTERGPYNFDLGGTPFSAGVENDGSGKLREPETRWG
                     GLMRNLETNDFEAANIEFIEFWVMDPFDNRGPFGTQVTSDGYLYINLGNVSEDVLKDS
                     RMFFENGLPKDGSTDALDQTNWGNVPRTPPITTAFDNDVESRANQDKGYDGLNSDEEA
                     DFYNQFMLDAPTYVSGAALVKLESDPSTDDYHYYRGGDYDDAELSILERYKKYNNPQG
                     NSPTTESSPEAYPTAATTIPESEDLNDDYTLNETESYFQYRIKLEPGMESINPYITDV
                     VVAEHTFQDGSSDSIKWYQFKVPIDQFDGKVGSIQDFKSIRFMRMFMTGFTDDVVLRF
                     ARLELVRNQWRRYQFSMLNPGEYIGNDDGNETDFNVSSVSIEENSARQPIPYALPPGI
                     SREQTLGSGSSVSTYQQNEQSLSMQICPLQDGDARAIFKSLNIDLRRYGRLTMNVHAE
                     PLPDAPLTTLNDGDLHIFIRLGSDFTNNYYEYDLPLTVTKLPLPAGTTEDEAVREAIW
                     ATLIDFPLDSLVQVKTLRNKNEVSPLVPFSITSGTDQGTYTIIGNPDLGYVEEIMIGV
                     RNPKDIGGSGAEFCSEVWVNELRLSNIDESGGWAALARADVKLADLGNFTLSGNLHTA
                     GFGTLEQQIDERYKDNFYQYAASLSLELGKFLPQGVNLRLPLYASISESFSNPEFDPY
                     ELDVDLQQKLDEISTTYGSDSSDLYKRSVIDYIAVKSINLSNIRFERGDKAGDPHLWD
                     KENFDLTLAYSQEYNSNPILEYDIVDRYKAALGYSYSQKAKYILPFEKVIDKKYKWLT
                     PVRDFNFNLLPTGYSFRTELNRQYGETLMRDVYGDGLIDPTYDKYFTWDRFYGLKLEP
                     AKSIKIDFSATNNARIDEPYGALDTKEKRDSLWNSFYDFGRNINYRHTVSATYTLPIS
                     KLPIFSWITIKANYNTSYGWVAGSIGIADTLGNTINNTQSKSVNGEFNFRNLYNKSKF
                     LKQYNSNTSPKAEKKSGKGGEDGADEKTADENTKTAKKPSTPTVSPPVETLVRLLISL
                     KRMTVNYSQNYATTLPGFMPSSDILGLNAATTAPGWDFIFGYQPSYKWMDSIARKGWI
                     SSATSLNYLFLQTYSENLDVRANFEPLKDFRLDLNLNKTYSKNHSEYFKNTNSIDDPL
                     FSHLNGVDAGSFNISYSIMGTVFEKLDAKEVSATFKQFEANREIISARWAALNTIYAD
                     DIFFNPLDGTFLPNYTEGYGPYSQDVLIPAFLAAYTGKDANEVSLNPFDQMPKPNYRI
                     TYNGLSKLPGFKRLFSSFNVTSAYTSTLALNSFITDLDFDGNYFAYGHVVDTLTGNYV
                     TLYDIPNIIVNEALSPLIGFDVTWINGVTSKFDFKKSRTLTMSFIDYQLTQINSTEFT
                     IGAGYKMSGFTFPFKIRGKRPNLENDLTFKFDFSFRDDITTNFRLDQDLNEPTGGMKS
                     ISLSPTIDYVVNDRFNVQLYFDRQQTIPKISTSYPITSTNAGVKVRFSLAE"
BASE COUNT        16251 a        11165 c        12082 g        15345 t
ORIGIN      
        1 tgcaaaaaga atatgaaata tcacaagccc gaaaaataaa ataatcgctg caatcaatag
       61 tagtccaaaa acccactgat tatcctgtaa cttaaaattt ttaaccttca ggctcaacat
      121 cggaatgtca ctcaccatga gataacttaa caatacgatt gtgcctaata acacccagtg
      181 atttgttagc gttgctgtca tgcccatttc attgcgcaac aggatcaatg gcaacgatac
      241 cacaaccatg gccatagcag gagaggggag gcctctgaat tctgtagttt gcctgctgtc
      301 gatattgaat tttgccaatc gcaatgcggc aaatagtgtt actgaaaacc ccgcagcgta
      361 atataaaatg ggatattcaa acgaattgta atgtgcataa tatgctcttg ccaataattc
      421 atacatgacc aatccgggca ctaatccaaa ggtgaccata tcagcaagag aatcaagttg
      481 ttttcctagt tcagattgca cttttaacat gcgtgcgaca aagccatcaa aaaaatcaac
      541 aacgcctgcc agcatcacgc aataacaagt aagatgtatc aaccccatgt ctatgagcat
      601 atcgtcttgt ttgctcatga tcaccatatg atcattgtat aaatacacca cccccataca
      661 acctaaaaac aggttgaaca gtgtcatcaa atttggaaga ttttgtatta atcgcatatg
      721 atgtgtgatg taaacaatga aggttgattg attatttgta tatggtaata attatgggcg
      781 attaattatt tactgttcat gatagtttca atctcttcaa cttcagatgg tgtgtctttc
      841 atgaagtcat atttactgcc atcaggcatt aacataatgt cattttctat gcggatgcca
      901 ataccttctt cgcgaatata aattcccggt tcacatgtca atactgctcc tgccggaatt
      961 ggttgatagt gatcgccgat gtcatgcaca tctaaaccaa gaaaatgtcc ggtgccatgc
     1021 ataaagtact ttttgaaggc cggccattcc ggattttgat ctgcaatatc ctgtttgctt
     1081 attaacccca atcccaacaa ttcggattcc atcaccttgc caacctctgc attgaaatct
     1141 tttaatatca tgccgatatg catcatatca atcgcttttt tctgcacccg caatacggca
     1201 ttgtatacgt ccttttgtcg ttgtgtaaat tttccggaaa caggaatggt gcgcgtcaga
     1261 tccgcacaat aatttgcata ttcaacacca aaatccatta acagtaaatc accatcatta
     1321 caacgtaaat tgttgtctac atagtgtaat acacaagcac ttggtccgct ggcaatgata
     1381 cttccgtatg cgggtctggt gcctctattg cgcaaaaatt cgtgttgtat ctctgcttca
     1441 atctcaaact ccatcacacc cggctttacg aaattcaata cgcgacgata agctttattg
     1501 gtaatgtcgc atgctatctg cattaaatcg cgctcccaaa tacttttaac cgtgcgcaat
     1561 tttgccatga taggcgcact gcgttcatat ttatgcattg gaaaacgcaa tttcgtttct
     1621 tctataaatc gcgtatcgcg tgttggcact ttaatcgatg cacgatcatt ttcatttgtg
     1681 tttaaataca catgctcggc atgattcatc agcataggaa aaacggtatt gaaatcctca
     1741 acccaatgaa tggtttctat cccacttgct gcgcgtgcct ctgcttcggt gtatttatgt
     1801 ccttcccata tggcaatatg ctcgttggtc tttttgagaa ataatgcttc tcggtatttt
     1861 ggattcggac tctcgggata aatcaataaa atcgtttcct cctgatcaat gccacttaag
     1921 taaaaaatat cggaattttg tttccaggga tgtgtttgat cagcattgaa cggcatttca
     1981 tcattgctgt tgaaaatggc aatgctgttt ggctttaaat gtgtggtata ttgcttgcgg
     2041 ttctgaacga aaagctgact atcaatcggt aagtacttca tgttggtgtc tattacaggc
     2101 ttaaaggtag aaaaaaatgc ccaccatgcc cgtgacccct tgcctgagcc tagtgtcaac
     2161 ctcgtcatat tcaaggcttt gtatcctgaa agttaaatat tcctctaatg ccttccagaa
     2221 ctgcaaaaat tgagcaggaa gtcgtagttt tataaacctg tgttttgaat aaacacgggt
     2281 acgacattat ataaattcat cattaaaacc ggtttacatg aagctcctca aatgcttact
     2341 tcccttatcc ctcctttttc tgatcacgca gagttgccag aagaccgatc aactgatccc
     2401 ggatgcggcc attgaaacat caaatgatgc cttccgattg atgactcccg catattaccg
     2461 cgaatctgaa aatggcagtt caattcaagt caggtttttt gaatcgccac gcattttcga
     2521 tttgcctaaa aacagcgctg aatacgcaaa tattaaatca ctcatcgata tcagtcgcga
     2581 cgccaaacag cctatgcgcg tttattttgg tgatatcact atgccttata tcagcatcac
     2641 aggtgcccaa aaagcaactg ccgaagaaat tagattaaat gaacagaaat ttgtcatcac
     2701 ccccgataat gctaaactca agaaagtgtt gccgggcatg gctgtgatga acgggatatt
     2761 tgactattgt gcagcacagg gctgtacaac tggaacagca actatcgact attgcattcc
     2821 ctttcagtat gtagttgacg gatgttatgc gcgcgctcat aaaatgcgcc agatcatgaa
     2881 tgaagaatat aattatgaac cacagaaagt tttcagctat gaaggtccat caggctctct
     2941 tgcagtagat gcgggcgatt gctgtgtata ctggtggtac catgtggcgc cgcttgttac
     3001 tgtaaaaacg gctaccggaa ctgcgcaata cgtaatagat ccaagtatgt tcgatgagcc
     3061 tgtaagcatt gctacgtgga cggctgcaca agagaatttg ggttgcacac ccttcgccga
     3121 cttcggatat accgagatca cagcaggaaa aatttatgca ccgggaggtt caacagataa
     3181 ctcatatatc agcacaaact ggacgctgca gttttatgct gatctggtta cctgccctta
     3241 agttcagcta ttgctacaaa caaaaagcga cctcatgtga agtcgctttt tttatgatta
     3301 accccctgtt aatattaaaa tagcattgtt gcacgcaagc ctatctgctg cgggaaataa
     3361 cgtgagtcgt tttctggatt gtttgtgttc actaccatgt ttttaatacc attctggaaa
     3421 aagccggtaa atactaatgt tttatcattg tcaagtccaa attcggcacc cgcgcccatg
     3481 cttaaactgg tattcgaccc ttcatagtca aagctgctgc caccgttttt gccatcgttt
     3541 accgtgattt tctctgtcag cgtgaatccg agtgttagtc catattgagc aaataaaact
     3601 gcttttccca attcatctga cctcaaacgt aataatatag gtaagtctaa tgttgtaata
     3661 ctgtaatccc aggcttttcc atttgatgcc aagccctccg gattgtcttt aaatgcaccc
     3721 ggtgcactgg taaagttcaa tcccgattgc aaggcaaacc gttcagcatc acctaaggtg
     3781 aggtctattg ttccaccaac ctgaaagcct actttcatgc catcaggtac gtagttatcg
     3841 ccttctacta accaaaaaga aacagttgga gccacattgg cgccgtagcg aaattgttga
     3901 gcacttgccg caacagctaa agaacagaat ataatcgtga gtattttttt cattgttatt
     3961 cgtatagaat gttgagaatg cctgtttaat taaaatagtc ccgcaatgcg aataccggtc
     4021 tgattgagca ggatatcgga acctacatta ttgtcttcat acacattcat gattcccttc
     4081 tgataaaaga atccgataat gatagaagca ccttctgcaa cttccacttc agctcctaag
     4141 ccaaactgaa gacctgcatt cttatcaaga taatttatac cgttagcatt gtccttttcc
     4201 aaataatcaa tatcggagtc gagtgtaagc ccgttcagag tataatcacc aacagctctc
     4261 aggcatttac cataagtggc tccaccttct ccatagattg tgaagctgcc aaactggggt
     4321 gtcctgagct tcaatgtaag agggatttct aaatatcgag ctctcactgc ccattcttta
     4381 tcgtcgcctg caccattgtt gtcggttgat gtcatgccgc tctgcgagta gttcagattc
     4441 aagccaaggg aaacaccaat gatgtcgcct ttaacaaagg tttgatctaa taataagccg
     4501 tattgaaatc cggaaaagga aaccgccgga tcgtacatat ctgcgggcag aacgagttta
     4561 ttgctgccgg gagcaatgct gaagccgtag cggaattgct gagcccggct tgaaaaagct
     4621 acgagcatca ggaggagaaa ggtgaattta cgcatgtctg aatttgaaat ttctacaagt
     4681 ggcaaaaata tatgctggtt cagcaattct caggtatttt gatggcattc atgtcataaa
     4741 attgaaagaa atttgcaaac atggttgttg ataatattga gttacagatc atttttagtt
     4801 aaaatataga tgatttgtta tgtcacaaac gataaaaaac agacagtatt ttagtttttc
     4861 agctgttttc tgttcttcca aaggaaaggg taatttgatg ggtttgcggc tcttttcata
     4921 gccaaaccgt aggtgttttg gttgagatct ggcaggggta cggcctttac aggaggaaaa
     4981 gcggcaattt atttggattt ctgtgtacaa cctcatatct ttgcagcccg tttattcgga
     5041 aatgactaaa tgggacgaaa aaccggccgc aggttgatta tgggagtgaa ccccgatatc
     5101 gttcgcaaat ggcttacctg cactctctcc tacaaatttt gggggtttgt gcacatacct
     5161 ttgtgaatat cctcacccct tcttatcttt gcagccccaa attttttaaa acgaaaaggg
     5221 taattaacga atgcctacta ttcaacagtt agtccgtaaa ggaagggacg taatgaaagc
     5281 gcccagcaaa agtcgtgcac tggatgcctg tccgcaaaaa cgcggtgttt gcaccagggt
     5341 atatactacc acccctaaga agcctaactc agcgcttcgc aaggtggcaa aagtgcgttt
     5401 gaccaatcag actgagataa tcgcctacat tccgggtgaa ggacacaacc tgcaggaaca
     5461 ctctatcgtg cttattcgcg gtggtagggt gaaagacctt ccgggtgtgc gctaccatat
     5521 tgtacgtggt gctctcgata ctgccggtgt aaacaaccgc aagaaaagcc gctctaaata
     5581 cggcactaaa cgtcctaaag caggtgctgc tccagccggt aaaaagtaat taagtaacag
     5641 acagtagtcc attataaaca atgagaaagc ttaaacctaa gaaaaggata ctccctgccg
     5701 acccgcagtt caacgatgcg acagtaacac gcttcgtgaa tatgatgatg tggcaaggta
     5761 aaaaaagtac tgcctatacc tgtttctatg atgcattggc attggtagaa aaacaaaccg
     5821 gcgaaagcgg ttatgaggtt tggaaaaaag cattgagcaa tgtaactccg ggtgttgaag
     5881 tgaaaagccg ccgtatcggt ggtgctaact tccagattcc tacagagatc cgccccgatc
     5941 gtaaagtgtc actctctatg aagtggctca ttatgttcag ccgcaaacgc agcggtcgct
     6001 caatggcaga gaaactggca gcggaaatcg ttgccgcttc taaaggagaa ggtgctacag
     6061 tgaaaaagaa agaagacgta cacaagatgg ccgaggcaaa caaagctttc gcccacttta
     6121 aatcgtaatc aagtcaagga ataattatgg caagggattt aaaatatacc aggaacatcg
     6181 ggatcgcagc ccacatcgac gcaggtaaaa ctacaactac cgagcgtatc ctcttctata
     6241 ccggtatgac acataagatc ggtgaggttc acgatggtgc agctaccatg gactggatgg
     6301 agcaagagaa agaacgtggt attaccatta cttccgctgc taccactaca gcctggaaat
     6361 acaataaaga agaatataaa gtaaatatca tcgatactcc cggacacgtt gactttaccg
     6421 ttgaggtaga gcgctcactc cgcgtactcg atggtgttgt ggcgttgttt tgtgcagtag
     6481 gcggtgttga accacagtct gaaactgtat ggcgccaagc aaaccgctac cgcgttccac
     6541 gtatcggttt cgtaaataaa atggaccgct ccggtgccga tttcctcgac gttgttcgtc
     6601 aagtgaaaga agtgctcggc gctaatcctg tgccactggt tctccctatc ggtgcagaag
     6661 aaactttccg tggtgtggtt gacctgatcg ctaaccgcgc tatcatttgg gatgaggaaa
     6721 cgcaaggtat gacttacaaa gaagtgccta ttccggatga tatgaaagac gaagtgatcg
     6781 aatatcgcgg caaactcgtt gaagctgttg cagaatacga tgacgcatta ctcgaaaaat
     6841 tcttcgacaa tcctgatact attactgaag ctgaattgat cgaagctatc cgtaaagcaa
     6901 ccattgatat cgctatcatt ccaatgctct gcggttcttc attcaaaaat aaaggcgtgc
     6961 aattcatgct cgacgcagta tgtcgttacc tcccttcacc actcgatatc gatgctgtga
     7021 aaggaactaa ccctgatact gaagaggaat tactccgccg ccctgatgct aaagaaccat
     7081 ttacagcttt ggcgttcaag atcatgaccg atccatttgt tggtcgtctt gcattcttcc
     7141 gcgtgtatag cggtcgcctc gatgcaggtt cttatgtact taacgtgcgc accggcaaaa
     7201 aagaacgtat cagccgtatt cttcaaatgc atgccaataa gcaaaatccg atcgacttta
     7261 tcgaagccgg tgatatcgga gctgctgttg gttttaaaga tatccgtaca ggtgataccc
     7321 tttgcgatga ggatcatccg atcgcactgg aaagtatcaa attccctgat ccggttatcg
     7381 gtctcgctat cgagcctaaa acaactgccg acgccgataa actcggtatg gctcttgcta
     7441 aattggcaga agaagaccca acattcaaag tgttcttcga tgatgaaacc ggacaaacag
     7501 ttgttcgcgg tatgggtgag ttacacctcg agattatcgt tgaccgtttg aaacgtgaat
     7561 ttaaactcga agtaaaccaa ggtgctcctg aggttgctta taaagaagca tttaccaaag
     7621 tacataaaca tcgcgaaacg tataagaaac aaaccggtgg ccgtggtaaa ttcgctgata
     7681 tccagtttga aatcggccct gctgatgaag gtgttatcgg tttgcagttt gtaaacgaca
     7741 tcttcggagg atctattcca cgcgaatttg tgcctgcagt tgaaaaaggt ttcaaagaag
     7801 ctatgagcac aggggtattg gccggttata atgtcgacag catgaaagtg cgcctgttcg
     7861 atggttcatt ccacgcagtt gactctgacc agttctcatt cgagatgtgt gcgcgtatgg
     7921 cattccgcga agcaggtcgt caggccggtc cgcaattgct tgagcctatc atgaaactcg
     7981 aagtgatcac acctgaagat catatgggtg atgttatcgg tgaccttaac cgtcgccgcg
     8041 gtatgatcga aggattcgat aacaaaaaca atgctaccgt gatcaaagct aaagtgccac
     8101 tgagcgaaat gttcggatac gtaacacaat tgcgtaccat cacttcagga cgtgcaactt
     8161 cgatcctcga attctcacac ttcgcacagg caccaaacaa tattgccgaa gaagtgatcg
     8221 cgaaatcgaa aggtaaagta tcagtagata attaaacaga aaagaaatca gtagtatgac
     8281 ctataagatc agaatgaagc tgaagtcgtt tgaccacaca ttagtggaca aaacagctga
     8341 gaagatcgta aagaccgttc gcaataccgg tgccgtggta acgggtccta ttccgctgcc
     8401 aacgcaaaag aaaatctata ccgttttgcg ttcgccacac gtgaacaaga aatctcgcga
     8461 gcaattccag ttatgcacct acaagcgttt gctcgatatc tacagtgcta ccaacaaaac
     8521 tgttgacgca ttgcagaaat tagaattacc aagtggtgta gaagttgaaa tcaaaatcta
     8581 acctcatcag atcatgaaag gaataattgg caaaaaagtc ggcatgaccc actatttcgg
     8641 tgaggatggt aaaatgatta cctgcaccgt tgttgaagcc ggtccatgtg tggtacttca
     8701 gaaaaaaact gacgacactg acggatacaa cgctattcaa gtaggcttcg gcgaaaagaa
     8761 agagaagaac gtaagtgccg gaatgaaagg acatttcaaa aaggcaaatg cacaagcttc
     8821 aactaaaatt cgtgagttcc gcaatttcac cgaagacaaa aatgttggtg ataccattac
     8881 ggtagatatt tttgaagaag gtgaaacagt gcacgttgtt ggtacttcaa aaggtaaagg
     8941 tttccagggt gttgtaaaac gtcacaactt caatggagtt ggtatgcgta cacacggtca
     9001 gcacgatcgt caccgcgctc cgggttctat cggttcatca tcattcccgt cacgcgtatt
     9061 taaaggtatg cgcatggccg gacgcatggg taacgatacc gtgaaaatgc gcaacatcaa
     9121 agtagcaaaa atcatttctg ataaaaacct catcctgctc aatggatcag taccgggaag
     9181 caaggggtcg tatgtaataa tcgagaaata aaatgaaaat cgacatcgta aaaatatccg
     9241 gcgaaaaaac cggcaggcag gtagagctta acgacgccat tttcggtatc gagcctaacg
     9301 atcatgcgat ttacctgagc gtaaagcagt acctcgctaa tcagcgccag ggaacgcaca
     9361 aggcaaagga aagaagcgaa gtagcaggtt ctacacgtaa attgcaccgt cagaaaggaa
     9421 caggtggatc acgtaaaggt gatatcaaaa accctatctt ccgcacaggt ggtactattt
     9481 tcggcccacg tccccgcaat tatagcttca agctgaatcg caaggtgaaa gaattggctc
     9541 gtcgctcagc attagcatat aaagcacaac aaaatggtat cgtggtggtt gaagacttca
     9601 acttcgatgc gccaaaaacc aaaatgtttg caggtatcat gaaaagcctc ggcatcagcg
     9661 ataaaaaatc attggtagtt gccggcgaaa atgaaaacgt gatgctttca atgcgcaacc
     9721 ttccaaaggt gagcagcaca cgcgcagaag acctgagcac ttatagcgta atgaacgctc
     9781 agtgcctcgt gttgacagaa agcgctgtga aacagatcaa tcaatcggca gaataacaat
     9841 taattgagat aataagatgg caaagcgaat cttaataaaa cccctcatca ctgagaaaac
     9901 aaccaagctc aacgaagagc atggtgcgta tggctttgtt gtgggattag atgccaacaa
     9961 aatcgagatc aagaaggcag tggagaaaca ctacggcgtg aaggttgaga gcgtaagaac
    10021 tgttatggat ccgggtaaat tcggatggaa cagaacaccg ttcggcatca gtcagggaac
    10081 caaaggacgt atgaaaaaag catacgttac tttgaaaaaa ggagaaacta ttgatttttt
    10141 cagcacaatc taatctgaaa gatggcatta aagaaatata atccggtaac accaggtacc
    10201 cgcttcagaa taggaaacgc ctattcagaa gtgaccacgg atacaccaga gaaaagctta
    10261 ttggctccgc acaaaaagtc cggcggtcgt aacagctcag gtcaccttac catgcgctat
    10321 atcggcggtg gtcacaaacg caaattccgt gtaatcgact tcaaacgcga taagcatgag
    10381 ataaatgcag ttgtgaaaac tgtagaatac gatccaaacc gtactgcgtt catcgcattg
    10441 cttgtatatg ctgatggtga gaagcgttat atcctcgcac caaatggtat ccaggtaggc
    10501 caaacagtac agtcaggcct gaatgctgct cctgaattgg gaaatgcatt gatgctccgc
    10561 aatattcctc tgggtactat catccacaac atcgaactct atccgggtcg tggtggtatg
    10621 atcgctcgca gtgcaggaag ctgggcacag ttgtcggcaa aagaaggtaa atatgttatc
    10681 atccgtatgc cttcaggcga aacacgtatg attcttgata cctgctttgc aactgttgga
    10741 acggtatcta atcctgacca caacttacag caactcggta aagccggtcg caaacgttgg
    10801 ctcggtcgtc gcccacgcaa ccgcggtgtg gccatgaacc cagtcgatca cccaatgggt
    10861 ggtggtgaag gacgttcttc aggaggtcac ccacgttcac gcaacggtca gatcgcaaaa
    10921 ggtctgaaga cacgccaacc gaagaagaca acatctaagt tcattattag cagacgtaaa
    10981 aaataattct ataagtaatg gcacgttcga ttaaaaaagg accatacgta gatcataacc
    11041 tggccggtaa agtggcacag atgaatgaat caggcaaacg cactgtgatg aaaacatggt
    11101 cgcgccgttc taccatcctg ccggaaatgg ttggccatac tttcgcagta cacaatggaa
    11161 ataaattcat tcctgtgtat gtgacagaaa acatggtagg ccacaaattg ggtgaatttt
    11221 caccaactcg caactttaaa agtcacgctg gaaacagata attattatgg aagcagtagc
    11281 aaaactcatc aacaacccaa gaacgaacaa caactcaggt tcgccgcgta aggctcgcta
    11341 tgttgttgac atgatcaggg gcctcgatat cgataaggca ttgagcgtgt tgaaatatac
    11401 aaaccgtcgc aatgtaactg cagtggaaca aacactgcgc agtgccatta agaactggga
    11461 aagcaaatac gaaatggatg ctgccgacag caatttgtat gtgaaagaag catttgtaga
    11521 tggtggaaaa accatgcgtc gcttccttcc tgcaccacat ggtcgtgctt accgtatgcg
    11581 caagcgtact aaccacctca caattgtggt agcatctcga aatgcaaaca atcaagaaaa
    11641 aaacgcataa caatataact gattcgaatg ggacaaaagt gtaacccgat aggcaacagg
    11701 ttaggtatca tccgcggatg ggacagcaac tggtatggcg gcaaaacaat ggccgagaaa
    11761 ctggttgagg atgaaaaaat ccgtcagtac ctgaatgccc gtatctcgaa aggtggtatc
    11821 agccgcatcg taattgagcg cacgctgaat cgtatcaccg taacaattca tacctcacgc
    11881 cccggtatca ttatcggtaa aggtggtggt gaagtagata agctgaaaga agagctgaaa
    11941 aaaatcacag aaaaggaagt acagatcaat attattgaga tccgtcgccc tgaactggat
    12001 gccaacatcg ttggcgaaac aatcgcacgc cagcttgaag caaggatcaa tttccgtcgt
    12061 gccattaaaa tggctgttgc ttcaaccatg cgcatgggtg ccgaaggagt gaagattcgc
    12121 gtgagcggtc gcctcaacgg tgctgagatg gcaagaactg aagaatacaa gcaaggacgt
    12181 gtgccgctgc acaccttccg tgccgatatc gattactcat ggaaagaagc tcataccgta
    12241 tacggaaaaa tcggtgtgcg cgtgtggatc tgtaaaggtg aagtactggg taaacgcgac
    12301 ctgtcaccaa acgtaggtat gtcgcaggaa aaagaacgca gcaaacaagg aagaagaaga
    12361 ccgaataaac gataaaatag aacgcaatca tgttagcgcc taaaaaagta aaacatcgta
    12421 agacattcaa gggtaagatg aagggtgatg ccaaaaaagg ctcccaactt tccactggat
    12481 ccttcgggct gaagacactg gaatcaggtt ggattaccga ccgtcagata gaagcagcaa
    12541 gggtggcttt gacccgtcac ttgaaacgtg aaggtcaggt ttggatcaac atctttcccg
    12601 ataagcctat cactaagaaa cctgcagagg ttcgtatggg taagggtaaa ggtaaccccg
    12661 aattgtgggt ggctgttgta aagccgggca gaattatgtt cgaggcagaa ggtgtgccac
    12721 tggatactgc acgtgaagca atgcgtcttg cagcagggaa attaccggtt aaaacaaaat
    12781 ttgtagttcg cagagactac gtagcataat taaaaaaata gcaatggcta agttaaaacg
    12841 agaagatttg aatcagctca gcaatgagga cctgcaacaa cgcttgcatg atgattcaca
    12901 gcacctcaca aagttgcaat tcacacatgc agtgaaccca atcgacaacc cgtcgcagtt
    12961 gcgttcaatc cgcagagaga ttgcaagaat taaaactgag atacgcaaac gagaaattgc
    13021 tagcgccaaa taaaataaat aggcatggaa caaagtacca gaaagttaag aaggaccatt
    13081 atcggtgtgg tgaccagcga caaaatggat aaatccatta cagttactgt ggaacgtaaa
    13141 gtgatgcacc cgaaatacgg taagttcctg aagaaatcaa aaaaatacca tgcgcatgat
    13201 gaagcaaatg atgctaaatc aggcgacctg gtgaaaatca tggaaacccg tccgctgagt
    13261 aagctcaaaa gatggagatt agttgacatt gtagaaagag ctaaataaca tgatccagca
    13321 agaaacaaga ttaaaagtag ctgacaacag cggagcacgt gaagtattgt gtatccgtgt
    13381 tttgggaagc acgcgcagga gatatgcatc gctcggcgac aagatcatcg taacggtgaa
    13441 agatgcactg ccgggtggcg gtatcaaaaa aggtactgtt tcaactgcag tagtagtacg
    13501 cacacataaa gaagtgcgtc gcaaagacgg aagttatatc cgtttcgacg acaatgcatg
    13561 tgtgttgctg aatgcatcag acgaaccgcg cggaacacgc atcttcggac ccgtagcccg
    13621 cgaattaagg gataaacaat ttatgaaaat cgtttcactg gcacctgagg tgttataact
    13681 atgaaagaaa caaattacag caatcgcttc cagccaaagt atcatgtgaa aaagaatgat
    13741 accgtagtgg tgatcgccgg tgccgataaa ggcaaaaaag gccgtgtgat agaagttgac
    13801 tacgctaaaa atcgcgtaat ggtagaaggc gtgaacatcg tgtctaaaca cacacgtcct
    13861 actcaacaat atcctaatgg tggtatcatc aaaaaggaag caccaattca cattagtaat
    13921 gtgatgcccg tagatcctaa atccggaaaa ggcacccgcg ttggccgtaa agaggaaaac
    13981 ggaaaatcag taagatattc taaaaaatca ggagaaatca ttaagtaatg gaaactaaga
    14041 gcccgcgtct taaaaagcaa taccatgaaa cggtggtgaa ggacttacag gagaaattcc
    14101 agtataaaag ttctatgcaa gtacctaaac tgttgaagat cgcaatcaac caaggtttag
    14161 gcgctgctac ccaagataaa aaagtggtag acgtagcact cgacgaattg acgcgtatta
    14221 ccggtcagca cgccgtgtcg acaatttcta aaaaggcgat ctcgaacttc aaacttcgtg
    14281 agaatatgcc aatcggtgca cgcgtaacac ttcgtggtaa caatatgtat gagttcctcg
    14341 acagattgat ttctgttgca ctgccacgtg ttcgcgactt caaaggcgta aacaacaaaa
    14401 gcttcgatgg tcgtggaaat ttcacactgg gtatcactga acaaatcatc ttcccggaga
    14461 tcgatattga taaagtaaca aagatcaccg gtatggatat cacatttgtg accacagcaa
    14521 ctaccgatgc tgaagcttat gaattattaa aaggattggg aatgccattc cgcaaagcat
    14581 aatataaacg catgtccaag aaatcagtaa tagcaagaaa taacaagcgc gagcaaatga
    14641 tcatcaaatt tgctgagcgt cgcaagcaac tgaaagccga aggtaactgg gaagctctag
    14701 ataaacttcc acgtaacgct tcaccgatac gctacaaaaa tcgttgtaag cttaccggtc
    14761 gtccacgtgg ttatgttggt gacttgggaa tttccaggat taaactccgc gaccttgcat
    14821 tgtatggcaa gatcccgggc atgacaaaag caagttggta atccaaagca gtaaaaaaag
    14881 caagagatga aagtaacaga tccaatagcg gattttctga ccaggatcag aaatgcccaa
    14941 aaggcaaacc accgtatcgt ggaaataccg tcatcgcaaa tgaaacagcg attgacagag
    15001 atcctgtatg aaaacgggtt catttataaa tataagttcg agcaggaagg tcctcaggga
    15061 actatcaaaa tcgcacttaa atatgacccg gtaacaaaat cgcctgccat aaaaggcatc
    15121 gagcgtgtaa gtagcccagg tttgcgtcgc tacacaggtg tggatagcct cccacgtgtt
    15181 atgagcggtc tcggactggc cattatgtca acatcaaaag gtgtaatgac agaaaaagaa
    15241 gctcgtcgcc aaaatgttgg tggtgaagta ttgtgttatg tgtattaatt aataaagaaa
    15301 gcagatcatc atgtcacgaa taggaaggtt acccatccaa ttacccgccg gagttacact
    15361 aagtgtggct ggtaacaata tggtaacaat taaaggaaaa cgcggtgagt tgaccttgca
    15421 ggttgacccg gatatcacgg tgaaaactga agacaacgct attaaattag agcgtccgtc
    15481 agagcaaaaa cgccatcgcg ctatgcacgg tttataccgc gccctcattg ccaatatggt
    15541 taaaggtgtt tccgatggat tcaagaaaga acttgaattg gtaggggtag gttacagggc
    15601 agcgaatacg ggccagatcc tcgagatctc tgttggatac tctcacccaa tcatgatgat
    15661 gatacctaaa gaggtgaaat tatctaccgt gagtgaaaaa ggtaaaaacc caactatcat
    15721 actcgaaagc aacgataaac aattgcttgg agcgatagct gctaagatcc gttcattccg
    15781 taaaccggaa ccatacaaag gaaaaggtat taaatattct gacgaagtat tgcgtcgtaa
    15841 ggcaggtaaa actgccggta aataatcggt aaaactatgt tgagcaaaaa atcaattagg
    15901 agacaaaaaa ttcgctaccg tatccggaag aaaatcgccg gaacggccga gtgtccgcgc
    15961 ctggcggtat tcaggagcaa caaagagatc tacgtacagt tgatcgacga tgttgcaggc
    16021 cacacattgt gcgctgcttc gagcaatgac aaacaaggta aagcttatac cggtactaag
    16081 gtagaaaaag ctgctgccgt gggaaaacaa atcgctgctc aggcacaagc aaaaggcatc
    16141 accactgtga ttttcgatcg cggaggatat ttgtatcatg gacgcgtgaa atcacttgca
    16201 gatggtgctc gtgaaggagg tctccaattc taaaaatgca ttttatctat ggcagttaat
    16261 aaagatatac aaagagtaag ggccagcgag ctcgaattaa aagagaagat cgttggtatc
    16321 agccgcgtag ctaaagttac caaaggtggt cgtacattct cattctctgc acttgttgta
    16381 gtgggtgatg gaaacggaac cgttggtcat ggtctgggaa aagcacgcga ggtaaccgag
    16441 gctattcaaa aaggtattga tgacgctaag aaaaacctca tcaaagtacc ggtgttcaac
    16501 ggaactattc cgcacgaaca acacggtaaa tttggtgccg gcaaagtgat gatcaaacca
    16561 gcagctcatg gaacgggtgt aattgccggt ggtgcaatgc gcgcagtact tgaaagtgcc
    16621 ggagtaaccg acgtattggc gaaatctttg ggatcatcaa atccgcacaa cgtggtaaaa
    16681 gctaccctcg atgcacttgc tcacctgcgc agccctctgg ctgttgcaca acatcgcaat
    16741 gtgtcagtga aaaaagtatt taacagttaa taataaccgt gtaagctagc gatatggcaa
    16801 agattaaaat tacatctgtc aaaagcacaa ccgacagacc aaaaaaccag aaggctacaa
    16861 tgaaggcctt aggtctgcgc agaatgcatc agaccgtgga ggtggaagcc accccacaga
    16921 tcattggcat ggttagagta gtacagcatt tggtaaaagt agaaaacgta taacatggat
    16981 ctttcaaact taaaaccggc ttccggttcc atcaagaagc gccatcgtat tggtcgcgga
    17041 caaggttccg gctggggtga aacctcgaaa cgcggtttca aaggtgcagg ctcacgttca
    17101 ggtacatcca ctaaacgagg attcgaaggt ggacagatgc cattgcaacg ccgcctccct
    17161 aaacgtggat tcaataacaa gaatttcgaa acgaaatatg ttaccctgaa cctcgatcaa
    17221 atccaggaaa tggtcgataa atatgagatc actaaactgg atcaggatat gctggtcgca
    17281 aacagaatca tcaaaggtga tgataaactg aaagtgctcg gcagaggcga attaaaagcg
    17341 aaagtggaaa tctgggctca tgcatgcagc gaaacagcac aaaaagcaat cgaagctgcc
    17401 ggcggtacca ttactatgat cggataacac atgaaacaat ttattcaaac attaaaaaat
    17461 atctggagca tcgaagatct ccgcaagcgt atcatctaca gccttggtct catcctgctg
    17521 tatcgcttcg gttcttacgt gatgttgccg ggtattgatg caggcatcta caaccaggct
    17581 gctaacgtag atagtcagag cggtttgctc ggattaatca acttgtttgc aggtggtggt
    17641 tttagccgtg cctccatttt tgcattaggt atcatgcctt atatctcagc atctatcgca
    17701 attcagttgc tgggtattgc agttccttat ttcgctcgcc tccaaaaaga aggtgaaagc
    17761 ggacgccgta agatgaatca gtacacgcgt tatcttaccg tgatagttac cgcattgcag
    17821 gcatcaggat attatgcata cctgaacact atgtctaaaa atgccgactt catcgtggca
    17881 cctcaaggct tgttctggtt cattacagtg gtgattctta ctgcaggaac cttattcaca
    17941 atgtggatgg gtgagcgtat cactgataaa ggaattggta atggtatttc catgattatc
    18001 atggtgggta ttattgccgg acttcctgct gcattggtga atgagattac acatcgtggc
    18061 gctgcttccg gcggtggatt tattccattc ctgctggaga ttgcattcct gattgtagtg
    18121 atcatgggtt gtattttgct tgttcaaggt acaagacgaa ttcctgttca atatgctaaa
    18181 cgagtagttg gatctcgtca atacggtggc gtacgtcagt atattccatt gaaaataaat
    18241 gctgccggtg taatgcctat catcttcgca caagcattga tgtttattcc cggcttattc
    18301 gcacaaataa atgaagattg gcagtcatca ccggtattgg ttatgctgaa cgattttcaa
    18361 agtttaggtt ataatttgat ttatggtttc ttgattgtat tgtttacgta tttctatacg
    18421 gcaatcatca tcaacccatc acaaattgca gatgatctta aaaagaatgg tggatttatt
    18481 ccaggaatta aacccggaaa gaaaacatca gaatttatag ataacgtttt atccagaatc
    18541 actttgccgg gatctatttt ccttgcactg gtagcgatat ctcctgtgtt gacacgtgct
    18601 tttcatgtag acccttcatt tgcaaggttc tttggtggaa catcattgct catcatggta
    18661 ggtgtaattt tggatacatt acaacaaata gaaagtcact tgttgatgcg tcactacgac
    18721 ggattgatga aatccggtcg tattaaaggg cgcacaaacg cacccgttag catacaacag
    18781 ggatgattta ttataaaaca acagatgaga taacactcat cagaaaaagt tctttacttg
    18841 tttcagaaac gctggcaatg atagccggca tcatcaaacc cggtgtaaca acattgtggt
    18901 tagatcagca agcggaagct tttattcgcg acaatggtgg taaaccatca tttaaaggat
    18961 ataaaggata tcagcatacg ctatgtgttt ccgtaaatga tcaggttgtg cacggcattc
    19021 cgtcggcacg cgaaatcaaa aacggtgatg tagtatcggt agattgcggt gcgtttctca
    19081 atggttttca cggcgactcg gctttcacct ttttagttgg tgaagtgagc agagaagcgg
    19141 tgaatctggc aagggtgaca agagcctgtg tatacaaagg catcgagttt gccgtagccg
    19201 gaaatcgcgt tggcgatgtg agtcatgcca ttcaactgta tgccgagcat gtaaatggtt
    19261 acggcgtagt gcgagaattg gtaggacatg gtatcggaaa aaacctgcat gaagaacctg
    19321 aagtgccgaa tttcggagcg aagggaaaag gtcctaaaat gcaggaaggc atggtgatag
    19381 ccattgaacc aatgatcaat cttggatctc ggaatgtggt gcaggaaaat gatggatgga
    19441 caatacgcac gcgcgacgga aaagtttctt gtcattacga acataccgta gcagtgatga
    19501 aaggaaaggc agatgtgttg tcgagtttcg aaatcattga tgaagcaatg gcaaagaaca
    19561 gtaatttgta tacagaaaaa gaaatagtag gttaatgtca aaacaggact taataaaaca
    19621 agacggcacg gtaacggaag cattgtcaaa tgccaacttt cgtgtgaagc ttgaaaacga
    19681 acacgaaata ttggcaacaa tttccggcaa gatgaggatg aattatatcc gcatattacc
    19741 cggtgatcgt gttacagttg agatgagtcc atatgattta acaagaggaa gaattattta
    19801 tcgttataaa taaaccggca ttatgaaagt tagaccgtcc attaaaaaac gcagcgctga
    19861 ttgtaagatc gtgcgcagga aggggaagct gtatgtgata aacaagaaga accctaaatt
    19921 caaacaacgt caaggttaat tttaaattac tgaataaaca atatggcaag gattgctggt
    19981 attgacttac caaaaaacaa acgaggcgta ataggcctta cctacatcta cggcattggt
    20041 gacaccactg cagcgcagat attagctgct gccggtgttg acgaaagcaa aaaggtgaag
    20101 gattggaatg atgacgagat caacgcgatt cgttcataca tcaacaacga attgaaagta
    20161 gaaggcgcac tgcgttctga tatgcaattg tctattaagc gattgatgga catcggttgt
    20221 tatcgtggaa ttcgtcaccg taaaggattg cctgttcgcg gacaacgtac aaaaagtaat
    20281 gcacgtacgc gtaagggtag gaagaaaaca gttgctaaca agaaaaaggc acctaaagga
    20341 taatccaggc aatttgccgt ttcatttcat ttatctctgt atacaatggc taaaacaacg
    20401 tcagcaaaaa agacaacaaa gaagcgtgtg gttaaggtcg acgcggaagg acaggcacat
    20461 atcctggctt ctttcaacaa cctgatcatc accttcacta acctcagcgg acaaacaatc
    20521 agctgggcaa gtgccgggaa agcgggtttc cgtggttcta aaaagaatac accatatgcc
    20581 gcgcaggtag cttgtaccga tgcagcaaaa gtggcatacg atgccggttt gcgcagggta
    20641 gaagtgtttg taaaaggccc cggttcaggc cgtgagtctg cgatccgcac catcgttgcc
    20701 aatggtatcg atgtatctgt gatccgcgac cttacgccaa tgccgcacaa cggttgccgt
    20761 ccgcctaaaa agaggagagt ttaatcaatc actgaaaatc aactagatca atggcaagat
    20821 ataccgggcc aactaccaaa aaatcaaggt cgttcggcga atcaatcttc ggttatgaca
    20881 aagcgttcga gaagcgtaaa tatgctcccg gacagcacgg tctttcacgc aaaaagaaaa
    20941 gtgcgagtga atacggcgtg cagctgaaag aaaaacaaaa agcaaaatat acttatggtt
    21001 tgctggaacg tcaattccgc aaaacctttg aagctgcaac tcgtcgccac ggcgttacag
    21061 gtgaagcatt attgcaattg cttgaagctc gcctcgacaa cacagtgttc cgtttaggaa
    21121 ttgctcctac tcgtcgcggt gcccgtcagc ttgtaaatca caaacacatt gaagtgaatg
    21181 gccagatttg cagttcacct tcggcaatat tacgccccgg tgatgtgatc actattcgtg
    21241 aaaaatctaa agatcacgaa tggatattgg ctgctgctaa caacacagga aaacgttatt
    21301 cctggttgga gtggaatcct actcaattaa aaggtgtgtt tgttaattat cctgagcgcg
    21361 atcagattcc tgagaatatc agagaacaac ttatcgtcga actctattct aaataatgtt
    21421 aaaaactatc tgaccatatg gcattacttt catttcagcg tcctgataag attactttac
    21481 aaaaagctac agacttcgaa ggtacatttg aattcaaacc tctcgaaccg ggctttggcg
    21541 taactatcgg caacgcatta cgcagagtgc tgcttagctc tctggaaggt tttgcgatct
    21601 cttctgtaaa gatcagtggt gttgaccatg aattcgctac tatcaatggc gttatggaag
    21661 acattaccga gatcgtactc aatttgaaac aggttcgcct gaaaagagaa ttgggaggtg
    21721 acgaaaatgc agttgaaaag atctttatct ccttaaaaga taaagaagaa ttcaaagcag
    21781 gtgatattga aaaacatact aatagcttcc gcgtaacaaa tccagatttg cttatctgca
    21841 gaatggaacc atttgtaaaa ctggatatcg aattgactat tcagaaaggt cgcggttatt
    21901 tacctgcaga ggaaaataaa aatgtagatc gtactatcgg tgttattccg atcgatgcta
    21961 tctatacgcc aattaaaaac gtgcgttaca cgattgaaaa cacacgtgtt gagcaaaaaa
    22021 cggactacga aaaattattg atcgagatca aaactgatgg cactatccat cctgaagatg
    22081 cgatcaaaga agcttctcgt attctgatcc aacacctcat gctgatcacc gatgagcata
    22141 tcactttcaa tactgttggc gaaaaagaaa caaatgttgt tgatgagcat accctgcata
    22201 tccgcaaatt gttgaagact ccactcgaag acctcgatct ttcagtgcgt gcctataact
    22261 gcctcaaagc agctaaaatc aattcgcttg cagaactcgt tcgctatgac actaatgagc
    22321 ttttgaaatt ccgcaacttc ggaaagaaat cgctcgttga aatcgaagca atggtgctcg
    22381 ataaaggtct ccagtttgga atggacctcg ctaaatacaa tttggatgaa gattaattca
    22441 gtgtttgtcg cactgctgta aaacaaaaag aaaatgagac acggaaataa aatcaaccac
    22501 ctcggtcgta caaccgctca ccgcaagtcg atgctcagca atatgggaac atcgctgatt
    22561 ttgcacaagc gcattaaaac tacgcttgct aaagcaaaag aattgcgtgt atatatcgaa
    22621 ccgatcatta atcgtggaaa ggaagacact acacacaatc gtcgtatggt gttttctgaa
    22681 ttgcaaagca aagaagctgt tcaagaactg tttaccaata tttctggtaa aattgctgag
    22741 cgcaatggtg gatatacacg tattttgaaa atcggtggta accgcgtagg agataatgct
    22801 gaaacatgct acatcgaatt ggttgattac aacgaaaata tgctgaaaac agctaaagcc
    22861 ggtggtgcta aaaaacgcag tcgccgtggt tcaggaaaaa cagagaaaaa agctgttgaa
    22921 cctgaaacta aagctcaaga tgctgctgaa ggtacttcag aagagcaagc ttaatttttc
    22981 agatataaga tatagcgggg tgatgattat cattaccccg tttttttatg tacctgcgat
    23041 aaagagtttt ttaccagcct tctgattatt tccggttaac tcaacgatgt acatgccttg
    23101 aggtgcatcc accataattc tgctgtcttt ttgaagaata ttatcttgca gaattaattg
    23161 cccgctaagg ttatatacat tcaatagata ggcgtttttt ggatcttcat tgagatgtat
    23221 aatgatatta tttcctgaag catacatctc aacttctaat aattccggat ttgttattgc
    23281 agttggttga agaaaagttt caatagaagt attagcatta ttttcccagg caaaatcttt
    23341 cactgtaaat gcgcgatttg atggatcggt agtgagtctt atccagccgt aatatgtgct
    23401 gtcgccaact gccaattgta atcctacaaa tttatcttcc tgtccaaata cccaatcgcc
    23461 accggcaaaa atggggtaat ataaatatga cccgatcagt gcatagaatt gataagcgag
    23521 tgtctgatat ggattggtga gaaattgacc tgcatatgga ccgatattaa ttcctgagcc
    23581 aatggcatat gggtaggcat aagcgcctcc gctaccggta aatgccgcaa ttgcattatc
    23641 attttgtggt gccgcaaata tgccattgat gatataccag ttcagtcctc caaaataggt
    23701 gaacacagct gagtttgcgg aggaaaaaaa gaaattaaag tcattcgttc catcgccatt
    23761 taaatcgaga gaaattccgg ttccgggttc atttacgagc acatcagggt caacgtcggt
    23821 gtagaccaca tcggccggag caaccatggg aattacagct gcagcgagtg ttgcatatgc
    23881 tgtgagggtc attccttgtt gctttgactt gtgcatacct ataaaacgaa aaaacctcct
    23941 gaaggttgcc tcaggaggtt ttatttaacg atcttactgc ttattggttg atcaatacgc
    24001 gttttgcgta gttagcacct tcggcagtgg taattttaac agtatatgtt ccggttgcaa
    24061 ccgcatcgag tgtgatttgc atacctgaca tgttcaggcc attgctgtaa acaacgcgac
    24121 cttgtatgtc gtatacattt acatcggcag aagttgtgtt caattcatta acaccaatgt
    24181 tgatgatatt tccattagca tatacattca actgacttgg atcgatgttg tgaattgcag
    24241 taggcaattg tcctgcttca atcgcagttt caggagtgct gaggtaagca taatcatcaa
    24301 tagtgatttc aaggtcatca ttatttacag atacgcgcac ccaaccgtag tattcattgc
    24361 catcgccttc aaagcgtacg ccaacaaatt ttcctgtttt tcccggccaa gcaccgaaag
    24421 tatagaattg ataattagat ccaacaccgg gataatcctt aacgctaagg aaagcaccca
    24481 tatagatagc tgaatttgta tagaaagcct gaccgccatc aatatccgcg cctgcattca
    24541 atgcttcggc atatgctaaa gtagcgccgc ctgttgttga aagcggataa gcaacaacac
    24601 cattgcccgg agtaggataa attttcatta cgttgaataa tccgtcaaag tatactgagt
    24661 gataagcaac agtatagaag aagtcaggta atgtgaaggt ggtgaaattg aaagtgaaat
    24721 cgatatcgcc attattatcc atgtctaaat catacacatc aagtggattc aaagtttcat
    24781 ccggatccag atctgtataa ccaacttgtc cttgtgctga tgtgtgcaat aaaaggaaag
    24841 tacctgccat tgatgaatag gctgcaagat ttttacggga taaccctgta gtaaaagatt
    24901 ttttcatttg aggtaagttt tcggttagtt agcagatgct aatataggtc tttttcaaat
    24961 ttgtagggag tgttacaaaa aaaaagcccc gacgcgatgg tcgaggcagt attgttactg
    25021 ttggtatccg attaattttg gatatacagt gatttgctga atgaagtatt tgctccggtc
    25081 aattggagag tatacaaacc tgtagcagcg ttatcaaggc tgatagccat gttgctgccg
    25141 gtaaggcgtg cagagtaaac caatttgcca tcgatgtcaa aaacattcac attgtcaaca
    25201 ccggcttcgc tgtctttaat aacaacgttg atagtgtttc cgtagctgta agcagcaacc
    25261 tgcgcttcag taaggttgtt gatagcaacg tctttttcgc ctgcagaaat atcagttcct
    25321 tcagcatcat cgtaagcata atctttgatg gtgaaagaaa caggtcctac agaaacatct
    25381 aaacgtgccc atccgtagtg gagtgaaccg tcgatgtcga attgaacgcc gatgtactta
    25441 tcttctgtat tagcgaaagg accgtaagtc acgccgctat agatagaagc aagccaaagc
    25501 atgttgtaag tgtttgatac aaaagatgca tcagggccga taaggtcgcc atcgttgagg
    25561 gcagaaccgt aagacaggat tggtccggtg taaccgatta ccatgttaga accgccaccg
    25621 tagccgtaag cgctaaggtt accgatcatg taagcccaag tccagttacc ggctgaatta
    25681 gagacagcag ccatcaagaa atcagaataa ccgttgttgt cgaggtcgag cgcatacgtc
    25741 tcacccaact ccacagtaac atcatcgatg tctgtgtaaa ctactgttgc gttggcttca
    25801 gaggtagtag ctacaaaagc tccggccatt gctgagtacg ctgctaactt tccagcgttt
    25861 tttgaattgt agagtttttt catatctgca gattatggtt aaaaattaca gggcgaatgt
    25921 aagcattttt ctgcattaca cacgccctgc ggactatttt ttgacaaatt tcatccttaa
    25981 caaggatgaa ggtcgtataa ttatatagac aggttcaatt gttttttaac tgtcaattct
    26041 gcattaccaa ggcgaactgc ataattgcct gaagccaagt cgttgcaatc caacacattg
    26101 tgtcctgata atccactcat ggtttttacg agcttgccat tcatgtcaaa taattgcaaa
    26161 tcagcagttc cggtgaaatc agatggaata tctacaacga tatttcctcc gttgctgaag
    26221 atctgtgcat ttgagagagg gttttcttca atctcggttg cagtctgacc aacgaaaatg
    26281 gcagtattcg gctcactttg atatgcatag tcgtggatgg taatcattgt tgcatcatca
    26341 cttacactaa cacgcatcca gccataatat cggtctttgt cgatattgat tctaaagccc
    26401 ataaaagcca tatcggtatc cagccagttt ccgccaacat aagtgtaata agggaatccc
    26461 aaaacagtaa cagaagcact cacagccatt gacattgcgc catttgcaaa gttgtcgtct
    26521 gatcctacca gtgtacctgc aggcagaatt ggtgcatata cacctgaata tccgctataa
    26581 gaaacaatgg atccaaggaa ttcattgcca ttcaaagctg aacccaatgc aatattcaca
    26641 acatatccga atgagaagcc gctataagtg ccgctaccgg agaagttata aatggagaat
    26701 gtgaagtcgt cggtgccatc ttcattgata tcaatcgaag cttcagcgcc ttcaccaccg
    26761 attgccagat ccgggtcgag gtctgtgtaa acaactgttg cgccggcttg attgatagcc
    26821 aggaatgcag ttgcgctggc agcatactgc aataattttt ttgttgaagt aaaatctttt
    26881 ttcataagtg gttgctatgt tcaaatatat gcgaaatctg caccaaaagt tacagtctgc
    26941 gccttaataa atgttaaatg gttagcatcg gtcaatttat ttacctgatt tttcgcggtg
    27001 aaggctttta tccactacag cagccatggc atccacattc atagtgcctc caaggaaagc
    27061 attgatcttt ttcaattctt ccgttgggtt attcaccaga ttgcgatgtg atacatataa
    27121 tatctccata ttttgttgct gcggcaccca ggtttgcaca cgctcgagat tttgtttgaa
    27181 tgcatttacg atacgcaaat tcaatgcatc ttcttttgct tttccatcgc gcaccaacat
    27241 gtgatgctgg ctcttaatca cttcatcgag atcgcgttcc ataaatatga tgcgatagcg
    27301 atagtttgca ggcagttcaa acaataaatg actaattact ttcacgcctt ttcccatggc
    27361 atctttcatc catgttttat tgcgtgcaag agatttcacc aattcgtgtt cgtaatatcc
    27421 tttaggatta ttttcatcag gtgtgcgaag accgtcagta aatatttcta atccaccttt
    27481 atcaagcatt tgcatcatca tacttgttcc tgaacgcggg agtcctgaaa caacaacgat
    27541 atcacctttg atataatctt taatctgtga tttaacttca acagcggctt caggtttttt
    27601 aagatgctcg ctataaatag ttgataataa ttgacgcgct ttattaattc ccggcgccat
    27661 tttcaaacac acttcaaatg cttcggcact gcgttcgtat tcgccgagat aatataatgc
    27721 ttcacccaag tgatagtgtg caaatgggaa gaaatatgtc aatcctactg cattcaaaaa
    27781 acaatcgata gactcttcgt aatgttttct gcgcaagaga gttataccta aaccatgatg
    27841 tgcagatgca tttcccggat cgtgctcaag ttctttgagg aaagcgcctt cagccttttt
    27901 ccaacgcttt aatgccatgt agcatctgcc taattgcatg tgcacgcgcg ccgcatttgg
    27961 catttctttt tcaatggctt cgaataattc catcgccatt ttaggtttat tctcaacgag
    28021 caataaattg ccttccatga ttttcatgct cacggattcg cgaccattat tttcagtctt
    28081 gattttagaa agcacttcgc gcgtttcttc cactttattc atcgactgat aagcatgagc
    28141 caatcgcata gcatagcgat tggtgccggg attttcgtca accagcttct tcagaatgtc
    28201 cactgcctca gcatattttc ttccgttcat ataagaacgt gcgaggtagt aattattttc
    28261 actaacagtg ctcttgattg ctttttcaat attttcactt ggcctttcaa tatatccaag
    28321 ttcaatgagt tgctccatcg cttcagcagc agcatatggg tcttcttgtt tatcggccgg
    28381 gtgttgacct gaatcaccgg ggatatcttc ccaggtatca atcttgtccg gaataacagg
    28441 tgtttccaat gcattaacaa gcacgtttcc atccatatcg gcagcaaccg gcaatccgaa
    28501 tagcgtaagc aacgtaggtg taacatctaa tactgatgcg ccataaatac gttcatcttt
    28561 tttaatgccg ggaccgttga gcacgaaaat gccataaggg ctatgttcca atgcaggagc
    28621 tgcaggttct tttggtaatt ctattggacg aagatggtca ggatggaaac cgtgatctga
    28681 aataatcata acagtaacat caggacctgc aagttcgagc aggcgctcca acatcatatc
    28741 atgatatttg taaccaccgg caacgacatc tttatataaa ttatataaat cgtccggtac
    28801 cattggcatt tgtggcggat ggaatttcat gaaaccatga cagaagtgat cgatggcatc
    28861 gtaatacact gcaaggaaat cccattcttc attttcaaga atataagttg cagcattatg
    28921 aatggttgag caatcggcaa taatctttgt gagcatgccc aatcgcttat ctttttcctg
    28981 atcaatttta ttcgcatccg gaataaaagg taaaatgtgc gatgctgtta attcagcagg
    29041 gtggatacgc atttttttga acagctcagc tttatctgca ggatgtacag ttccatcgag
    29101 cataggccat ggttcgttga ttggctttgt tgctctttga taaagattgc tgatcatagt
    29161 gccgttgatt ggctcagcag gatgtgaagg ccaccatccg atgacgtgac ttttcagatt
    29221 atgctgattg aagatattcc aaacagcttt tacttttctg gatgtaatat atacaggacg
    29281 gatacctttt cctgatggat ccggttcggt aaaaccaatg attccgtgtt tataaggacg
    29341 tttaccggta gcgatagaag tccataatgt tggcgacagt ggtggatcga gtgttgcatg
    29401 attaccaatc accccgccgt taactaactt ttctaaagtt ggcattaaac ctgcgtccat
    29461 cagtggattg atcaccttcc agtcggcagc atcccatccg atcagcagta ctttttttgc
    29521 aatacgttta cccatgttta attctaaagt ctaataaatt ggagataaaa aaatgagcca
    29581 atgcgtcttt taatctgcta ttggttcatc ttgttgttgt tcggtgtttc caccttgttt
    29641 ggcttctttc attttctgaa attgccattt agcctgacgc caggcgatga ggcctttata
    29701 tccgagcgcc agcaatccga gagatccttc caccgggatg cgaaacggtt tgccgtcttg
    29761 cgtggtgata cggagcttat tgccttgttt tttttccata cacgattaaa ttgttatcct
    29821 gacccaatgc aggcaggacg caaatttaac ctaaaccaag cggtaatgct aatatcagaa
    29881 tgatgatttt actcgcttca ggaggagtgc agtgcatgtg aagatcagcg gatcaaggta
    29941 atattccctt gcatatgctg cttcatgccg tcttccaact gcactatcag cataaaagca
    30001 taaacacctt gtgcaacagg ttcttcatta taagtgccat cccaacctgc cgatgggttg
    30061 ccggcgatca tattttcttt ggtgaatatc aattctcccc aacgatcata aatattcata
    30121 gatactatgc tggcgaggtc ggtgccatat gcgaagaaat agtcgttgat gttatcacca
    30181 tttggcgtaa atgtattcgg aacaaaaaca acaggatcga atacgggaac tactgtaata
    30241 gtaaccatat cactgtcgat gcaaccaaag gcgtcccaac tggtaaccat ataggtttga
    30301 ctgagcaggg agtcaaaaac aggagcaggg caatcggtgc aactcaatcc ggcaggaggt
    30361 tcccaaagga atgtagttcc acctgtagca gtgatcacgg tagttgtata ttcaggaata
    30421 tattgatcta ttcctgcgtc aacatcattt tgtatgacgt tgatcgcgcc ggtagtattg
    30481 tttgtacagc cattggcatc ggtataggta taggttacgg tgtataaacc gaggtcacct
    30541 gccaatgccg gatcaaactc gtcgccgata acaccttcgc cactaaatgt tccgcccgca
    30601 ggatcaccaa taatcagggt agggtcgcct tcgagacaaa tcacgtcaat gagatctata
    30661 gtaggtgttg gcagcgggta aatagtaact tctacggtgt ccacttccgg gcagccgggg
    30721 ctggtataat aaatttcata agggcctccg ggggtgcatg cagccaggtt gagttcaccg
    30781 gtagcagcgt tgatggagat acccgccatt gcgctgaaag tgcctccggg attaagaatc
    30841 atgttaggca gggtggttcc ggttgcacaa tatgcaggca gtaagtaact aaaagctgca
    30901 tcggtcaatg gcatgatctc aatcactacg cttgtgctgt tggaacatgg tcctgccggg
    30961 gtggtatacg ttatggtgta aaaggaaccg ggtatacctg ttgtaaggtc gatagcacct
    31021 gtaagtgcat taatagataa agtggccgga ctgacagtaa aattaccacc gggagtttca
    31081 acgctggtga caattgccgt tccataattg caataggaag cagcatcata tgtaaaacca
    31141 gggtcgtcca atggatcgat ggtaactgtt tcggtgctgc tgttgctgca aggccctgca
    31201 ggtgttgtat aggtaattgt atacgttgtg ccgatgtcac cacttgccaa attaagcatg
    31261 ccggttgttg aattgattcc catggttgtc gggaatatgc taaatgttcc acccggggtt
    31321 gttatagaag tcggcaatgt ggtgcctgtc gggcaatagg aaggagcgct ataactaaag
    31381 gtcggatcat cgagcgggtc gatggttatt gtcgtagttg tgctattgga acatggacct
    31441 gtcggtgttg tataagtaat agtataagtt gtgccaacag ttccggtagt caaatcaaca
    31501 actccggtgg taggatttac aaccagaccg gcggggctaa cggtgtatgt accgccggga
    31561 gtagcaacag ttggagttgc agttcccgtc gggcaataac ttggggcagg gtagttcacg
    31621 gaagggtcat caaaatcagt aatggtaatg gtgacactgt aagtgcttac acaaaggata
    31681 ccaactgtgt atgtaatagt gtatgttgtg cctatggtgc cggttgtgag gttaactaca
    31741 ccggtggttg ggttaacagt aaggcctgca ggagacactg aaaatgtacc gcctgctgtg
    31801 gtaataaatg taggtgtagt ggtaccggtg gggcaataat tgatatcgct atagccaaac
    31861 gatgcatccg ggtcaacaac aatggttacg aggtctacag ccgtttcaga agggcaatcg
    31921 cctgtaatgc cataagcggt ataagaaacg ctataactac ctggtgttga ggcgtttagg
    31981 tcaatttcac cggttacggg atctattacc attccggcag gaacggcggc aaaagttccg
    32041 cccggatcgc cggtaacagt tggcatggca tagccggtag tgcaataaaa nnnnnnnnnn
    32101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtgaggtggt tctcaataac atttgtggtt
    32221 tcgtttaaaa caatttggaa tttcccgtag agacctgtgc agctaaacat gggtacgtta
    32281 tcccaactta caacaaattt cctgtttgga gcagtgccta tggtttggta tttgatacaa
    32341 ctgccaacag gggtgcataa tccgggatgc cagtcttgcc atggtcccat gatgcaattt
    32401 tttggcactg ttccggcaac gctgggaata gctgctgagg taaaagtggt aaccaaagcc
    32461 cccggacctc caaaactcac ccagccatta gagcctatat aacattgtgt atattggttt
    32521 ccgaggaagc agaaagtaaa gcccagagga acagcagtag agtaactgtc atctgttaat
    32581 ccgccaccac cggcttggat tggagaagtg ccggcatatg tttccggact atagggataa
    32641 acttcgaagg tatagctgct tgtgccgtag gatggagaag aaataactgc aagggttgcg
    32701 gtgccgcctg cacaaatagt tgtatccggc gttacaacaa gctgtgcggg taatatgctt
    32761 gataagcaaa ttaccgataa aaaggccgtc aatttcctca tgtgctgtgc gagtccctaa
    32821 aagaggggaa agttactact ttttcaggat tgcaaaaaaa gttggggggg gctcaagtaa
    32881 tacaaaatga taggtactcg aaaaaaacaa aaccccggca gataaactac cggggttgct
    32941 ccccaacctg ggctcgaacc agggacctac tgattaacag tcagtcgctc taaccagctg
    33001 agctattgag gaatgtttca ctggaaacgg gatgcaaatt tacaatcttc cgtgtgcatt
    33061 gcaagtatgc cgcggaattt ttttctgaaa gctgatgaaa cgaagggatt gccgttactt
    33121 tgcagcaaaa aataagacat gagtttatta gtagttggaa cagttgcatt tgatgctata
    33181 gagacaccat ttgggaaacg tgataaagta ataggcggtt cggccaccta tatcggatta
    33241 tcggcgagct attattataa acctatacat attgtttcgg tagttggtag cgacttcccg
    33301 aaggcaacac ttgcagcatt caacaaaaag ggtattacca cagatggtgt gcaggtaata
    33361 aaggggcaga aatctttttt ctggtcgggt aaatatcata tggatatgaa ttcacgtgat
    33421 actttgcgta cggatttaaa tgtgctggca gatttcaatc cggtgttgcc agaaaaattg
    33481 cgcaagccca acatgttaat gcttggaaac atagatccta atttacagat tcgtgtgata
    33541 gagcaattaa aatcgcgccc gaaattaatt gcgatggata ctatgaattt ctggatggat
    33601 attgcgcgtc ctgctttaga taaagtgctt aagatggtgg atgtgttaat tattaatgac
    33661 gaagaggctc gtcagttaac aggtgaatat agtgttgtaa aggctgcaaa aaaaatcatg
    33721 aagaacggtc ctaaaacact gattataaaa aaaggcgaac acggtgcttt attattttcc
    33781 ggttcagaag tgttttttgc tccggcgctt ccattggaag aagtatttga cccaacaggt
    33841 gccggtgata cgtttgcagg tggatttatg ggttatttat cacagagcaa aaccttgtcg
    33901 tttaccaata tgaaaggcgc aattgtttat ggcagcgcaa tggcatcgtt ttgcgttgag
    33961 gagtttggtc ctgaaaaatt attggctctc aaaaagcctg agattgcgaa gcgtgtgaag
    34021 atgttttcaa cgttatccaa attttctatc aagggataag gcgaccaata atattgaatt
    34081 tttgcagtgt gatttctgtg tgtggggcgt cttttaactc gtcggtaagg tcttgtccgg
    34141 caaagtgttc gtagtgtttg ccatttcgcc ataaacgcga aggggtgaca tcgtaaatca
    34201 agcccttata ggcaatccag atttcttctt tatcttgacc attgcgcaag gcgagttgaa
    34261 atttattata ggcgggcaat gtgtttacat ccataaatga aattggttaa tggatgaaat
    34321 ttgagtaaag ccatcttgtg caaataaatg ttgcgctgcg atattatttc cttgcgtata
    34381 taaattcatg accggcaatt gatatgcatt tgcaaaatga tcggaggcgg tcaataattt
    34441 tttggcaatc ccctgatttc gcataccggg agcaacggct aataacccga tttgcatatg
    34501 gcttttttta tttgtgaagg ctatcagccc caaggggtta atttctgttc caataaaata
    34561 aatttcatcg cccattttcc catgaagtga ttcacgtatc cacatggcat acagagcagt
    34621 tgtttttttc ttattgaaat gatcatcaca cataaatctg gagtgctttc cggcatgtat
    34681 tccaagttca ataatgccat ctgtcacttg tgttaaaatt ttcacttcgg gatttaattg
    34741 aggtgcacgg gtttcgcgtt gaagcagaca tcgcgagctc ataggattta atgtagcgga
    34801 tgacctgttg ctaatttcga cctccgttgc gctatcaata tacaaacaag tgtagccttc
    34861 tgaacgccag ttccctactt gatgttcggt aattatgcag gtgctttgta attcaaggcg
    34921 accgatttta aaaccgaaga accggctgtc ccagtcgagg tattcgattt taatatcggg
    34981 ttccatatta catgactaat tttgagtgaa taataagact tagctttaca atgtcaattc
    35041 caatatgcta accatttcta acctcaccaa gtcgttcggc gatcatgttg ctgttgatgg
    35101 catttcgcta tcgatagcca agggaatgtt ttatggatta cttggcccga atggtgccgg
    35161 taaaactact acaattcaga tgatcagcag catattgcct cccgattccg gtaaaatttc
    35221 agttgatgga ttaggcgttt atgagcacgc atccgtaaaa atgcacatgg gtattgtgcc
    35281 acaggagatt gccttatacg atgaattaac cgcattagag aatttgattt tttggggtaa
    35341 tctatatgca gttcatggga agaccggaaa agatcgtgca gcatttttgc ttgattgggt
    35401 agggttggcg gaccgcaggc atgatattgt aaaaaattat tccggaggaa tgaagcgtcg
    35461 tgtgaatatt gctgccgcac tcatgcatga tcccggttta attgtcatgg atgaaccaac
    35521 agttggtata gatcctcaaa gcaggaataa aatttatgag ctgcttgatg aattacatca
    35581 tcagggaaaa acaattttat ataccacaca ctatatggaa gaagctgagc gtatgtgtga
    35641 taaaattggc attattgata aaggtaaaat cattgctgaa ggatcacttg atgaattaaa
    35701 aaaagcacac gcgcaggagg agaatattgt aattcatttt accggtgaag cggtaagcaa
    35761 gcttggtaat tatgttattt cttttccgga gccgcaaatg atgactgtag ttacgccaca
    35821 ggctcgaaag cgtttacatg atgttgtgca gttgtgtact gaagggggaa tcgacatcac
    35881 aggaatagat attcacgatg ccggattgga aagtattttt cttaacctaa ccggacgcca
    35941 gttgcgggat taatataatt cgtatgtgga ctattgctaa aaaagatatt tatattttct
    36001 ttaaggataa gcgtggattg tttttgtctt tattgttgcc aataggttta atcaccatgt
    36061 tcgcgtttgc atttggcgga atagggcaag atgatgaaga tccggcgcca atacatgtat
    36121 tatatgttga tgcagatcaa acgacttcaa gtaaatcgct tattgccatg ttggatacta
    36181 ttccgggaat tgcttgtttg tcaactacgg cggaagacgc tactgctaat attaaaagtg
    36241 gcgatgaaat ggcggcggtc gtaatcaaca aaggatttga aatggctttg caaacgggtg
    36301 aagatttgcc ggttgttttg caatacgatc aaagcagagt aatggagatt ggtgtgttgc
    36361 aacaattact tacaagcaga ttgtcggcaa tgaaggggtc ggtagatgca catagaggca
    36421 ttaataaaat tatgcagcaa tcgtttagta atatgccgcc aaatgtgcag gattcaattc
    36481 gaacatcact tgaagaaaat ttattgcaga aagaaaatca tgaacaatta ataagcatgc
    36541 aggaagtagt gggtgatgcc gaaagtaatt gggggttaat tcaggcggtg gcaggcacgg
    36601 caattatgat gttattattt agtgtgagca gtattggcaa gagtatgttg gatgaaaagg
    36661 aaagtggtgt gttgcgcaga ttgcttcaaa ctcccatgca tccatatgga ttattatttg
    36721 gcaaaatgat aactgctttg gttattgcgg tatttcaatt aaccatcatg tttttatttt
    36781 catggctggc atttggatta gatatattca tcaatatacc ggcactaata attatgattt
    36841 tggcaacctc gctggcatgt tctgcttttg gtgtattgct ggcaagtttg gttaccaccc
    36901 gaaaacaggc ggattcgtta agtactattc tgatattatt tatgagtgct gttggcggta
    36961 gcatgattcc ccttttcatt atgccggttt ttatgcaaaa tgctgcagtt gtttctgtta
    37021 actattggag cattcagggg ttttacgata tcttttggcg ccaggcgggt ttggaagcta
    37081 tagttggcaa tgcgctggtt ttaacaggta tttccgcagg ggttttgcta ataagtatgt
    37141 atttttataa aaaaaatatt ctcaggctta tctgaggtca attttattag tcagacattc
    37201 atgaaaagaa ttctcctctc ctgcataatt tttagcggat ttattgcaag tgctcaggat
    37261 gcattggatc catcatttgg tggtgatggt attgtaacac ttgattttgg cggtaatgat
    37321 attgcatatg cagtattgat tcagcctgat ggtaaaatac tggctgcagg tcaggcggga
    37381 attggcccgg cattaacaac tgatattgcc atcgcccgat acaacaccga cggaaccctt
    37441 gatccggtct ggggtggaga tggcactgta acgctcgatt tgggtgtaga ttacagcgac
    37501 ttttcatttg ctctggcaag acaacctgac agtaagatag tggtagccgg gtgtggcttt
    37561 ttcgattctg gttataagtt tgctgtgtta cgttatagag cagatggcag tcttgatccg
    37621 gctttctctg atgatggcat ttttatcact gattttggag gtatggacga ttgggccaga
    37681 gcagtgtacg ttatgcccga tggaaaaatt gtcgcctccg gttatcgcaa tgatggtttg
    37741 aaacataatt tcgccattat acgtcttacg ccggaaggag accttgatcc tacttttgga
    37801 accggtggga aagtgtatac tgtttttccc gtaaatcgcc acgatcaaag ctatgcctta
    37861 gctgttcaac ctgacggcaa aatgattctc gccggttata gttatgttgg cacaacagca
    37921 gattatcaat ttgcgttagc ccgttataat gaagatggtt ctttagatga tactttcggt
    37981 gatgcgggcc tagtgttaac acctgtccct atgatacagg gcgaaagcta tgccatggaa
    38041 ttacaacctg atggtaaaat acttgtatcc ggattctcat acaccgattg gaaaacagat
    38101 attgccatca taagatttaa tagcgatggg tcagttgaca ctgaatttgg taccgatgga
    38161 ttggtgaaga cagatattgc aactgatcgc gatgatcatg catacgccat gtacctgcta
    38221 gccgatggaa gatttcttgt tggcggaaac agatggaatg gttatgattg ggatatggca
    38281 ttattgcgat ataatgccga cggatcactt gataatactt ttagcgggaa tggcatgctt
    38341 acaattgcca ttggcgatga cgcggactgt attcaggcta ttgatgtaca aagcgatggg
    38401 aaaattgtag ttgcaggata tagcgataac gggcctaaca atgattttgc acttgtgcgt
    38461 tttatgcaag acggcggttc aggagttgga atcagcaatc aggtcgtaac agcaaatttc
    38521 agtatctcac cgaatccggg atatgataat atttctatca attgtacatt aagtggtatt
    38581 attgatattg ttgatatgca agggcatgtt gttgaacgtc gagaagtggt gtcgggagat
    38641 aacaccatcg acatacatca attaccgaca gcaaattata tcataagatg gcgaaatgaa
    38701 agtgcgcagt gcagtttgcc tttcatcaaa aaataatatt aatattattc ttcgttatca
    38761 gcgttcgttt ttttcacatc atatttaggt aaggcaaaca ggaagccgaa tgcctctccg
    38821 tctttacgtc ccaatttttt atgatgaatt ttgtgggcac ggattatacg ttgcatatat
    38881 ttactttcgg gacgcatatg gtgtttcata cgacgatgaa caagcacatc gtgaaagcca
    38941 aagtagcata atccgtatgc ggaaatcccg gttcccaccc aaaaaatcca attataagcc
    39001 ggcgacccga tatacatcaa atagattgca ggcgctgcaa aaatgatggc gtacaggtca
    39061 ttcaattcaa acctgccgtg gcgaggttcg tgatgcgagc gatgcaagaa ccagcctaca
    39121 ccatgcatga tatacttatg cataaaccac gcaaaagcct ccatgaaagc aaaggctgca
    39181 agaaaggcaa taatattaat gatgatgtcc atcggtagtt ataacaaagt tattggtctt
    39241 gtgttcatca tgcaccaatc agggcattct tttttttcga acgggatttg aatccttcga
    39301 tcagcataat attcatgagc ataaggctga aactgtacaa agaatcttct atggggatgg
    39361 tgcccattct gattccaagg ttttcatgat tattatacca aactacttct tcggcaattc
    39421 cccacccggt taaaattcca ttcacaataa aaaaggggat caggtgccaa aggtaaaatc
    39481 gataaaattt ccccatgtat gtgcctttta ccaaaaacaa ctgaatgcat agcaatacaa
    39541 cagcatatcc gcaattcaac gcagtatacg ctttattgta atttgcaacc aagaggatta
    39601 aagcgatagt cagcattgca tagcttatct tgtttgcata tttgtctaaa gttggtgtgt
    39661 cgggccaaat tgtatttgta aaagcgtaaa tgaaaatact tgcataagga acagtgaaga
    39721 aaaataatat ttcttctatc ggtaaattca tgaaatgggc gccggtaata tagcggtcat
    39781 taaatcccca aactccattg actgtaaata tttgatccca ggttaagaat acaagagccg
    39841 tgatgaacat agacggaaac agccatttcc accaattgac gtaacgcact ctggattcaa
    39901 aactgaagag aaatggaaac aggatggtgc ctgtattgat cagcagatac aggtaactct
    39961 ggttcatggt gcatagatac ctaaaacatt tgaacgcagg taaagagatg cccacaatcc
    40021 gatttttttt ggatttgaca ctcttattct tgtttgcata acagcttcgg gttctgtgct
    40081 tctgattttt ttgaaaagct cgagatagta tttatacgcc agataaacac cgaattttgc
    40141 accttccggc aattgcttta ttcctttgta ggcttcatca aagtcatttg cgatttcttg
    40201 ctcaatttgt gttttctgac gttcacagaa attttcgaaa tcaacatttg gaaaatatac
    40261 acgtcctcta tccttataat cagattgaag atctcttaag aagttcacct tctggaatgc
    40321 agcaccaaga aatctggcag gttgaattag tttttgataa tgcgtttcat cgttttcaca
    40381 aaatactttt aaacacatta gacccacaac ttcagcactg ccataaatgt attcttcata
    40441 atgatgttgt ttataattcg acatatgaag atccatttcc atgctgtgca agaatgcatc
    40501 tatcaaattt ctgtcaattt gatatttatg tacagtaagt tgaaaagcgt gtaatatcgg
    40561 gttcaatgaa attttttcat cgagtgcact ccaggtgtct tccttaaatc ttagtaaaag
    40621 ggtttgttta tcccaatcgt gaaaggtatc aacaatttca tcggcgtagc gaacaaatcc
    40681 atagatagca taaatagcat catgatatcg gcggtcgagg gctttaatac ccaatgtaaa
    40741 ggaagtgcta tacagctcag ttaccttgcg actgcaggtg aatgacgatt cgtggaatag
    40801 atgtaacata cgcctgatta ggacattaaa actaattaaa tacggaattg ttcactgacc
    40861 ggatcaatgt ccaacaagtt tttccatctc ctgagcagct acataccctg atatgagtga
    40921 tggtggcaca ccggggccgg gaacagtcag ctgtcccgcg tagcaaagat tccttacccg
    40981 cttgttacgc atttttggct taaagatggc ggtttgcatg agggtattgg ccaatccgta
    41041 ggcgttccct ttacaagcat tatatctgtc gacaaagttt ccgggagcaa actccctctt
    41101 ataaaccact cttgatttga tttcttcacc gcaatgtgct tcaagccggt ccattgcttt
    41161 atgatagtat tcctctctta gttctgcagt atccttgagt ccggcagcaa caggaaccaa
    41221 aatgaagaga ttttcatctc cggcaggcgc tacagtcggg tcagttactg atggtgccga
    41281 gacataaaat agcggttgtt caggccacct tggatcggta tatatttcat cagcaaattg
    41341 ttcaaaactc tgatcgaaaa ataaattgtg atgcagcaaa tttttaactc ttccctttac
    41401 acctaagtaa tatataatag cagaaggtgc aaagatcttt ttatcccaat atgtttcatc
    41461 ataattgcga tacggctggt ccagcaattt ctgttcaacg tgatgataat ctgcagctgc
    41521 tattattcct gacgcggcaa taaaatcacc atggactttg acgcctgttg atctgccttc
    41581 ctctacaata attttttcaa ccgggctatt aaactgaaat tgtacaccta gtccttcagc
    41641 tacacgctgc atgccttcaa ttattttata cataccgccc attggatacc aggtgcctaa
    41701 aacaagatcg gcataattta atatcgaata aagtgcgggt gtatttgatg gcaatgctcc
    41761 aagaaagtaa acagggaatt caagaatttt tctgatgcgt tcatttttaa atttggcata
    41821 tacctcactg cgcagagatg tgaataattg catcgtaaac aatgccttta ccatcctcag
    41881 ctcggcaaac tccataatac tattgcaggg ccgaaaaacg agatctttca tgcctacttc
    41941 atatttgtag gcagcgtcgt cgagaaattt ctgtaattga attcctgacc ccggttctat
    42001 tgattcgaat agtgccttta attccgaaat ggatgccgga atttccatca catcgccggg
    42061 tccgaaaaca atacgatagc caggatctaa tcgcttgagt gcatattgct gctcaacgga
    42121 ggatccgtaa gtattgaaaa accattcaaa cacatcgggc atccaatacc aacttggtcc
    42181 catatcgaag gtgaagcctt gttcgctaaa aacactgcat ctgcctcctg ccgattcatt
    42241 tttttctaaa atggtaactg aatatccctt cgctgccaag gcacatgcag cggaaatccc
    42301 actgaatcct gcgcctatca ctattatatg ctttctttcg gccataattg atataagcga
    42361 atgaaacagc taattcggtg aaatgtttag caggagcgtt tcaaataatc tccatcaggg
    42421 tgcgaaaggc gaagaaatca ttgccaaact tttcctgagt atcgcgctgt aatgagggcc
    42481 cagatgtaag tttataggtc aggaaatcat ccaaattgtt gagtgtgtat tgcacaacaa
    42541 aggtcattcc ttccatatca ccgtcgtcaa ccagacggca aagtcgatat tccacaaaga
    42601 aaccggtagc cattacatca gggatatgtt tctcgcgcat ccaggtaagc cactcgtcgg
    42661 ctctggattg ttcaattttt atggtgacgt tgtatagata catgtggtaa aaataagagg
    42721 aaaaaaggat ggttggttag caagtttgag ggttagcagg ttagcaagtg gtggggaatt
    42781 agcaaatttg ggaattagca gattagctaa ttgggaatgt gcttgcatga agacgctcgt
    42841 caaagtgagc aagtttgaga gttagcaaat tagcaagtgt gatgggcttt taacgctggc
    42901 gcgttaatag ggaatgcaat ttattgcttt gcaatatttt aattccgaag cgcaatcagc
    42961 tccctctcta tcgacgatag gagatggaga gggcgggggg tgaggcgggg taattagcaa
    43021 atttgggaat tagcaaatta gctaatgggg gtgaattctt gcatggagat tttcgtttta
    43081 gtgagcaagt gtgagagtta gcaaattagc aagtgtgatg ggcttttaac gctggcgcgt
    43141 taatagggaa tgcaatttat tgctttgcaa tattttaatt ccgaagcgca atcagctccc
    43201 tctctatcga cgataggaga tggagagggc ggggggtgag gcggggtaat tagcaaattt
    43261 gggaattagc aaattagcta attgggggtg tgctagcatg aggactattg tcatagtgag
    43321 gaagtttgag ggttagcaag ttagcaggtg aggtggtttc gcgcaaagac tgccaagggg
    43381 caaaggcgca aagtctgcta cacccgcaca tgaacacccg aacacataaa cacaacaatg
    43441 ttaaaatcca atttagctat ttgacagtag cttaaaataa ttgcttggat ttggaagtag
    43501 catttgaggt gatatatttg aagttattaa taccacagtt ggcaatgcat acccgacaat
    43561 ctacatatat acaaaggatc aatgatgcac ttagatatat cgatttacat atcgatgctg
    43621 agttgtcgtt ggatgttgtt gcgaaggtgg cgttctattc gccattccac ttccacaggc
    43681 tgtttaaggc tgttacgggt gaggcattgc tggtgtatat taccagaaga cgcatagaac
    43741 attcaggcct tatgttaatg caccgacctt caatgagtgt ttcagaaatt gcctatgctt
    43801 ttggattttc cagtgttgca gtttattcaa aaacctttaa aaaagtaatg ggtttgagtc
    43861 caactgcatt tcgtgatgaa cttaatatta aaaatagcaa gattggacaa ctcgacagca
    43921 agatcgggca agatgacctg ataagtgagg catacatttg caacattaat aatcttaaaa
    43981 aatggttaat gatgcaagca aaaatttcaa ttaaggacac acatgatctt cattttgcct
    44041 acctcactca aattggagtt gatggcattg aatctacatt tcaacaagta ctgaaatggg
    44101 ccgggcctaa agctattctt tcaaaggatg aaacgtttat tagcagagtc ttccatgata
    44161 gctttaaagt aacagatgaa agcaaggttc gcatgagcat tggaattgcc tcccgccatg
    44221 aactgccgga cgatcaacat atgagcagtg gtgttatatc cggtgggaaa acatttattg
    44281 gtcgcttcga aataacactt gaggaatttg aacctgcatg gaatagtatt ttcatagaaa
    44341 tgcatgatag gggatataac aaagcagatg gatttcctta tgaattatat cacaacaatc
    44401 cggcaaatca tcctgaaaag aaatacattg tggatctatg tattccaatt gagtaatttc
    44461 gatcaattta ttttatcacc acgaagctga cgatatcttt tgcgggcttc ggtgctatac
    44521 agcgagtcct tgaagttcat gaagatctgt tcgtaacaaa gttgggcttt ctcgttatcc
    44581 ttgaactgat attgataaat atctccgagc atataaatgg catcatctcc taataattcg
    44641 taggcataat tctgacgaat ttgttcaaga taatttactg cagttgtata atcttgttgt
    44701 ttgatggcga tttttgcttt taagaataga acatcatcac taagttgatg agcggggtaa
    44761 gtggttgtaa gggtatcgag cactttcaat gctccctgga tattattttg aaataaaagt
    44821 aaatctgcct ttgcatacag catcatcggt tcagcaatag aatccagtcc caaattatcg
    44881 gttatgaaaa cagaaagctg catggcgtca tttgaaatga gctcactggt agcagctttg
    44941 agcacatcca gcataccttg agcgaagctg aaatctcctc gataataggc aagttttgca
    45001 cttttaaatc gtgcttcttc tcccagcggt tcgtctttca tggctttatc aacttgagca
    45061 taaagaagag tagcctccca aacatcgccg ctgatgagat aaaaatcgcc gagatcaagt
    45121 ttaatgcgtg accgatccgc agcagaaatt gcgggccagt caaccagtgg ctccgccaat
    45181 gaaatagcct ttgcgaaatc agctgcataa aatgcttcaa gctttgctaa gtcgtcggta
    45241 acagatgcgg cgcgtgcatc ttttctgtca tattcggcta gaaaagcggt atagctgttt
    45301 cgcaacgcgt caatatcgga tttcgtgtat tggccaagtt tgaaaatttt atccttatgg
    45361 caatttaaaa ttccattttt tgcgctgaaa taatatggtg aatcttttcc ttttgaaata
    45421 atatattcaa atgctgtaat ggcagcatcg taagattctt cggcgcgcgc agtttctcca
    45481 agatcgtaaa cgcgttcgcc attttcttta ttgcgtttat ccagtgcctt agcctgtatc
    45541 agtgcgccat caaaatcttt ttgttgaata taatcccaaa tgagtaattc ggtgtattgc
    45601 aattcgtatt ttccattttg aatacgtgca aacaatttct cctgtaaaag tgtatggtct
    45661 ttctcttcat cgagtatacg cgaaaatgcc gaagtcactt ttgcacttcc acccatagga
    45721 tcttccacat agtaatcgag ataagtgtct agtgcttgat cgaaactccc tgcccgataa
    45781 tacaaaccgg caagttcgta gttaaaggaa taggtgctca cgatatccct cgctcgttca
    45841 taaacacgaa tcgcattttc cggcatgtca tatccaataa atgcattggc aagttgtgtt
    45901 acctgcggtc gactatccgt cattttttga atggcagatt catactgttt tgtagcattt
    45961 ttcttatcac cttgtttttc caatacattg ccatagtcga cataataggt cagattatcc
    46021 ggattttttt tgatctgttt gtcgataatc ttttctactt ccttataatc ctgtagcact
    46081 aacaaacagt tataataagc tttgtaatat ccatttggat tgtcattgaa cagattttta
    46141 tagagatcag ctgctttatc atattgaccg gtactcatat attgctgagc cagcgcagca
    46201 tccgtttgcg ctcctgctgt cagatagcag gataaaaagc aacatataat cactagtttt
    46261 ttcatacaat aagcgtatct aaaataatgc ccggattact gtttttagta ttccgacaac
    46321 acaaattaac ggaatcagct attatcatcc agagcaaatc caatttttat tcgttgatat
    46381 acccttacag gtttcccgcc atccatacct ggtttccacc tcctcattcc ttgtacaaca
    46441 cgaattgctt cctgatcgca gcctccgcct attccttttt ccactttcac atcagaaaca
    46501 ctgccatcct ggttcaccac aaatactacc caaactgtgc cggtaatatt atttttatga
    46561 gcgatttcag gatattgaac attatcggat agatattttt ttaatccttt tggtcctccc
    46621 ggatattggg gataaacttc tacattcgag taaatagtat ttccatcacc tgtaactgcc
    46681 gaattgtttg tgctgccgga agtggcggtg cctgttgaag tgctatcaat ttgcttatca
    46741 tcggtggtaa gaggcttgat atcgcttact tcattgtcgg gttttacttc catttcatct
    46801 accttctcaa gattctgtgg agcagcagcg ggtggaacgg caggtggaag cacttcgaaa
    46861 agcggcggtg gttcacttag ggtaacttca acattgctaa aatttgaagg aggtaagctt
    46921 cgatctatgg aaaaatcgat taaatgaatg cctgtataca aaagtgtaag agctgtcatc
    46981 aaatacaacc cgataaaggt atttctgcga atggttgagc ggagcacaaa ggagccgtag
    47041 cgtttgttgc gaccgtgaaa aacgatctca tcaaacgtgt cggttaaaaa tggtgatgat
    47101 tgctccattg acattcaagt atcctcaata caaaaaacat tccgaaagta acgctgttca
    47161 gcgaacttgc cgttttttgc taactgaaat taacaatacc aagccggcgc caagcaagca
    47221 gccgaggcta tttgctaaga tatcataaac gtcaaattgc cttgttggca gcgctctttg
    47281 tattaattcg atgctaattc catataaaat acataaacct atagccctaa aaatgctctt
    47341 tttgagggta tagataactg ttaagcggat taaagtctga gcggtgagga acgtaagtat
    47401 gccataaacc aggaaatgca tcaccttatc cggttcccaa attgacaaag agggtagctc
    47461 gtcgctcgga attaccgata caacaaggat aactaaagcc caaagaatgc ttaaaacccg
    47521 gaagattttt cctgaaaatg acatattagt gtccgatatg cgcctggtat tcagcagcgc
    47581 tcatcagtcc atctacctgt gaaagatcag acattgtgac cttgatcatc caaccatcgc
    47641 cataaggatc tttgtttacc gattcgggag cgctatccag tccggcattg atctcagtga
    47701 tctcaccgct aaccggcatg aacaattcag atacggtttt cacagcttca acggtgccga
    47761 aaacatcatt ctgattcata tgatctccca acccattcag ttctacatat acgatatctc
    47821 caagttcact ttgggcgaat tcggtgatac cgatagtgcc tgtagtgcct tcgataagga
    47881 tccattcatg gtctttggtg tatttgaggt gggcaggaaa ttgcataact gttcttttgg
    47941 gcaaaaatag gggttttggc cgaattgttt tagtgatttt tagtacgtgg gaaatcgttc
    48001 agcatttcag atgcttacat gaaattctta ctccattcgc ccttattcag caagactgaa
    48061 gcgaacctta acacccgcat tggtgctggt aataggatac gaagttgaga tcttaggaat
    48121 tgtttgttgg cgatcgaaat acaattgcac attaaagcga tcgttcacaa catagtcaat
    48181 ggttggcgac aatgaaattg atttcatacc acctgtcggt tcattcaaat cctgatcgag
    48241 acggaaattt gtcgtgatat catcgcggaa actgaagtcg aatttaaatg tgaggtcgtt
    48301 ttccagattt ggtcgcttgc ctctaatttt gaatgggaag gtaaatccac tcattttata
    48361 gccggcacca atagtgaact ccgtagaatt aatttgtgtg agctgatagt cgataaagct
    48421 catggtgagt gttctcgatt ttttgaaatc gaatttagaa gtaacaccat tgatccatgt
    48481 aacatcaaaa ccgatgagtg gtgataaagc ctcgttcaca atgatattag gaatatcata
    48541 taatgtaaca tagttacctg ttaaggtatc cactacgtga ccatatgcaa aataattacc
    48601 atcgaaatca agatcagtga taaagctgtt caaagcaagc gtagatgtat atgcactggt
    48661 cacgttaaat gatgagaaca gtcgtttaaa tccgggcaat ttgctcaatc cgttatacgt
    48721 aatgcgataa ttcggttttg gcatctgatc gaaaggattc aatgaaacct cgttagcgtc
    48781 tttaccggta tatgccgcaa ggaaagcagg aatcagcaca tcttgagaat aaggaccata
    48841 tccttcggta taattcggaa ggaaggtgcc atcaagtggg ttaaagaata tatcgtcggc
    48901 ataaatggta ttcaaagcag cccatctagc agaaatgatc tcacgattag cttcaaattg
    48961 tttgaatgta gcagatactt ctttcgcatc gagtttttca aatactgtac ccatgataga
    49021 ataactgata ttgaaacttc ctgcatcaac gccattcaag tgactgaata acggatcgtc
    49081 gatggaattt gtgtttttga aatattcgct gtgattttta gaataagttt tattcaaatt
    49141 taagtcgaga cggaaatcct tcagcggttc aaaattggca cgcacgtcga ggttctcgct
    49201 ataggtttga aggaacaggt aattgagtga ggtcgctgaa ctaatccatc ctttgcgcgc
    49261 aatgctgtcc atccatttat aggaaggctg ataaccgaaa atgaaatccc aaccgggtgc
    49321 tgtggttgcc gcatttaatc cgagtatatc agatgaaggc atgaatcccg gaagtgttgt
    49381 tgcgtaattc tgggaatagt ttacagtcat tcgcttcaag ctaataagca atcttactaa
    49441 ggtttctacg ggtggagaaa cagttggggt tgacggtttt ttagctgttt tggtgttttc
    49501 atccgcagtc ttttcgtcag caccgtcttc accacctttc ccgcttttct tttctgcttt
    49561 gggggatgta tttgagttgt attgtttcag gaatttcgat ttattataca ggttgcggaa
    49621 attaaattcg ccattaacgg atttagattg tgtattgttg atggtatttc caagtgtatc
    49681 tgcaatgcct attgaacctg cgacccaacc atagctggta ttgtaattcg ctttaatcgt
    49741 aatccaactg aaaattggca atttgctgat aggtaaagta taggtagccg aaacggtatg
    49801 gcgataattg atatttcttc caaaatcata aaaactgttc cacaatgaat cgcgtttttc
    49861 tttggtatct aatgcaccat aaggttcatc aatacgcgca ttgttagttg ctgagaaatc
    49921 aatttttatc gatttggcag gttctaattt caaaccatag aaacgatccc atgtgaaata
    49981 cttgtcgtat gtaggatcga taagtccatc gccataaaca tcgcgcatca aggtttctcc
    50041 gtattggcgg ttaagctctg tgcggaaact gtaacctgtt ggcagcagat tgaaattaaa
    50101 atcgcgaacg ggagtcagcc atttgtattt tttatcgata actttttcga agggcaggat
    50161 atattttgcc ttttgactat agctgtatcc caatgcagct ttatatctgt cgacaatatc
    50221 gtattctaag atcggattgc tgttatattc ctgagaatat gccaatgtga gatcgaagtt
    50281 ctctttatcc cacaaatgcg gatcacctgc tttatctcca cgttcaaaac gaatatttga
    50341 aaggttgata cttttcactg ctatgtaatc aatcaccgac cgtttgtata gatctgaaga
    50401 atcagaacca tacgtggttg aaatttcatc tagtttttgt tgcaagtcaa catccaactc
    50461 atatggatcg aattcaggat tactgaatga ttcagaaatg cttgcataca atggtaaacg
    50521 aaggttcaca ccctgaggca ggaattttcc caattccaaa ctcaatgaag cggcatactg
    50581 atagaaatta tctttatatc gttcgtcaat ttgctgctcg agggttccga atcctgcagt
    50641 atgtaaattt ccggagagag taaaatttcc cagatcagcc aacttcacgt cggcgcgagc
    50701 caatgcagcc cagccgccac tctcatctat attcgataaa cgcaattcat tcacccaaac
    50761 ttctgaacag aattcggcac cgcttccacc tatgtctttc ggattgcgca caccaatcat
    50821 gatctcttca acatatccaa gatcaggatt accgatgatg gtatatgtgc cttgatctgt
    50881 tccgctggta atactgaaag gaaccagtgg cgaaacttca tttttgtttc gcaatgtttt
    50941 tacctgcacc aaagaatcca gtgggaaatc gatgagtgtt gcccaaatgg cttctcgaac
    51001 tgcttcatct tcagtggttc ctgcaggcaa tggtaattta gtaaccgtta acggtaaatc
    51061 gtattcgtaa tagttattgg tgaagtcgga accaagtcgg atgaagatat gcagatcacc
    51121 atcatttaat gttgtgagcg gtgcatccgg taatggctcg gcatgcacgt tcatggttag
    51181 gcgaccatag cgacgaagat cgatgttcag cgatttgaaa atggcgcgcg catcaccatc
    51241 ctgtaatgga cagatttgca tgctcaatga ttgttcattt tgctgatagg tagatacaga
    51301 acttccgctt cctaatgttt gctcacggga aattccggga ggcaatgcat aaggtattgg
    51361 ctgacgtgca gagttttctt cgatacttac acttgatacg ttgaaatcag tttcattccc
    51421 atcatcgtta ccaatatatt ctccgggatt caacatagaa aactgataac gacgccattg
    51481 gttgcgcact aattctaatc gtgcaaatcg caataccaca tcatcggtaa atcctgtcat
    51541 gaacatacgc atgaaacgaa tagacttgaa gtcttggata cttccaacct taccgtcgaa
    51601 ttgatcgata ggcactttaa attgatacca tttgatagaa tcgctgctgc catcctggaa
    51661 agtatgttca gctaccacta catctgtgat atatggattt attgattcca ttcccggttc
    51721 cagcttaatg cgatactgga aataactttc cgtttcattt aatgtataat catcattcaa
    51781 atcttctgat tccggaatgg tagttgctgc agttggatat gcttcaggag atgattcagt
    51841 agttggtgaa tttccctgag ggttattata ttttttataa cgctcaagaa tacttaattc
    51901 cgcatcatca taatcaccac cgcgatagta atgatagtca tccgttgatg gatcgctttc
    51961 caatttaacc aatgcggcgc ctgaaacata agttggtgca tccagcatga attgattata
    52021 aaaatcagcc tcttcatcac tgttcaaacc atcgtaacct ttatcctgat ttgcacgtga
    52081 ttctacatca ttatcaaatg cagtagtaat tggtggtgtt cttggaacat taccccaatt
    52141 agtttgatcc aacgcatcgg tgctgccatc ttttggcaaa ccattttcga agaacatgcg
    52201 cgaatctttc agtacatctt cggatacgtt tcctaaattg atgtagaggt agccatcact
    52261 ggttacctgt gtgccgaaag gtcctctgtt gtcgaaagga tccatcaccc agaattctat
    52321 gaattcaata ttcgcagctt cgaagtcgtt ggtttcgaga ttgcgcatta agccacccca
    52381 gcgtgtttcg ggttcacgaa gttttcctga gccatcgttt tcaacacctg cagaaaatgg
    52441 tgttccgcct aaatcaaaat tataagggcc gcgttcagta ggataatatg caagatcaaa
    52501 agtgctgagt tgtgtcaaca cagttgtttc gtaatcagct tgaggaaaaa tttctttttc
    52561 atcgatctga atggtatagt gatttgaaag atcagcatca cttaagtgat caggtgttga
    52621 tggattattc tgttcgttga ataaagggtc aatattatac catgctaatt ttgctctatt
    52681 cattccatat gtaagactat cgaacaaccc tgcctcaggg aataagatag cattattttc
    52741 atctgtagca ccctgcggtg tactggccag tacccagctt gaataaggga atttcaaatc
    52801 atacgcactt ctgctgcctt cgaagtcgtc gatataaatt ctacctgctt catccttgcc
    52861 gattgcttta ctatgtcctg gttggaaacg tgcaacttca cctgtcaatg aaattgtaga
    52921 tgtttctttt gttccataga acggcaattt atccaacgca cgagtcagcc agggtgcatc
    52981 attagtgtaa ttgatatcga aaccataaat tgagttggag atagggtcat cgccaatatt
    53041 tactttttgg gtgtatggac gctctgataa atgcatccag gttgcaccta atgtaaagtt
    53101 gtcgtttatc caataatcta atcgcgaacc aacgagtgat ttagtttgaa aaccataaaa
    53161 ggcattattc tcgaaagtaa cattgatctg ttgacctgaa tttagtatcg cttcatttac
    53221 aatcttcaac cttcctaatg aatagtcgat ggtatagtca acattttctg tcagtaattg
    53281 accgcctgcg gtaactttta ccgacccttt aggtaagttg aatgcaccga gataaatttc
    53341 tgacgacacg cttgatttat attctcccga caacacatat ctgtcgtatt gcggaaattg
    53401 caatgcgata gtttttgttt cgttatacaa ctcatcataa gcgtattttt ctgcttccag
    53461 atcgtttcca aaaactgcat cacttcgcaa atagccgccg aatggttcca gtacaggaaa
    53521 gtacacacgt ccgttctgtg tgttgatggt gacgccattc acgaagtcga aaataccatc
    53581 cggttgaggg tcgttgttat tattcaaacg gtcgaggccc aataatttaa tcagtggtgt
    53641 gctgttaacg tttgaattat ttgcgggaat gaatcgcttc atacctccac ccggatcttg
    53701 atacacaaca tcgaggcgga aaccatcgct gctcacttgg aatgcaccaa gagagtaaac
    53761 gtttttcatc atcagatccc acaatggaat ttgagtaatc gccgatgtgc tcttcaacat
    53821 tttcatgaat agcacattgc tggtatctac tcccggaggt aaatcgctcg aaaattcacc
    53881 tacctgatac gtttgtccat tgtaagtata ttcataagca acggccaaaa catcatccgg
    53941 ttgtaatgtt gtgttcagtg aaatgaaacc taattgcgga ttaaatataa aatctgtagg
    54001 gtcgagtttg cgcattctcg ctttttcgta atcgcgcact gcctgcatgt taaatgccgg
    54061 gcctgtaagt gtgctgatca ctgtattcaa atcgcgagtg cctgcggtgc catataattt
    54121 ctggtagagg ttgttggcat tatttgacgg caaaccaccg ggagtcagag aacttactgt
    54181 tccggcagga tctatttcac cccaataaat actatccggt tcaccaaggt cagaaaaggc
    54241 aacaatgtcg cgcgtatttt gtgtttcacc tgtagtattg gttacccaaa cctcaatttt
    54301 agttacattg atcggtgagc tgacatatgg caatgtcttc aaccatcctt catatccttt
    54361 gcggaaataa tgcgacatga agaagtgacg gttttcgtcg tattgatcgg caaaaatttc
    54421 aaattcacgt ttttgtgcgc cgccttctat ctggatgctt tgtgtttgag atttttgttg
    54481 cgataaaata ttggtcatgg ttagtcgacc aaattgcaat tctgttttaa taccaaacaa
    54541 agaagtaggg cccggaatta attgagttgt caaaggcatg ctcacgttac cggcttcaat
    54601 accttgaacg atattatctt ctttgccttt gtattcgagt ttgatttgat tttcaaaatc
    54661 gaatgtagcc tgtgtgttat agttgaaatt taatttcagg ttattgccga tagatcccaa
    54721 aacattcgca ttgatatcca tatcgaaatc aaaaccaccc tgacgtcgct gactttcagt
    54781 gaggatagga ttctggatat tttgaaaact tccaccaaag gttaactcaa cgttaccctg
    54841 agggcgtatt tctacattac aaccatcgaa tatggcacat tctctgtcga atgggacaag
    54901 ttctatctta cctccggttc cgccgaggat gtttgatgca tct
//
LOCUS       DAOQHY010000002        50312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_234_length_50312_cov_6.005591, whole genome shotgun sequence.
ACCESSION   DAOQHY010000002 DAOQHY010000000
VERSION     DAOQHY010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 50312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50312
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_234_length_50312_cov_6.005591"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..498)
                     /locus_tag="PLL28_00320"
     CDS             complement(43..498)
                     /locus_tag="PLL28_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67781.1"
                     /translation="MQKFLQTILVVIGSLGVVISCNSGYDYWELSEFNMQPDALKDMA
                     EIKLVYYSNGPAQVDNDGAYIHAIVTSVETGDTINILSPYLSEFSPEDGDKTFIYIGI
                     HSNIGKAVSSDWDLDKYTKVIRDPKFDAIAHNDHPTVIGSIASTEMPFQ"
     gene            complement(562..1065)
                     /locus_tag="PLL28_00325"
     CDS             complement(562..1065)
                     /locus_tag="PLL28_00325"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF67782.1"
                     /translation="MDFKLRPWNMKDLPDLVKHANNPNIAGNMTNGFPHPYTKADGIK
                     FIHFATQDDPVHIFAIEVEGHAVGGIGMHPQTDIHCKNAELGYWLSEDYWRNGILSKA
                     IPLMIDFAFKTYDIERLFARPFGSNKASQKVLEKNGFKLEARLEKTIFKNGIFEDELI
                     YAVRRKK"
     gene            1313..1921
                     /gene="cysC"
                     /locus_tag="PLL28_00330"
     CDS             1313..1921
                     /gene="cysC"
                     /locus_tag="PLL28_00330"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HNF67783.1"
                     /translation="MAPVQDNIHPIFHRLLQRSDKEQLLKQTAKVIWLTGLSGSGKST
                     IAEALEKELHKMGFLTMLLDGDNIRTGINNNLSFSAEDRIENIRRISEVSKLFLNCGI
                     ITINCFVSPTNEIRALAKSIIGEADFLEVYVNAPLEVCEARDVKGLYAKARKGLIPDF
                     TGITAPFEAPEHPALEIPTDKLEIQEGVQRLMDFILPIISNQ"
     gene            1928..2833
                     /gene="cysD"
                     /locus_tag="PLL28_00335"
     CDS             1928..2833
                     /gene="cysD"
                     /locus_tag="PLL28_00335"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HNF67784.1"
                     /translation="MSYSLTHLRELEAESIYVMREVAAQFQKPALLFSGGKDSIVMVY
                     LARKAFWPAKIPFPLVHIDTGHNFPETMEYRDWLVKEMGATLIVGSVEDSINRGTAVE
                     ERGLNASRNALQTVTLLETIESNKFDACMGGARRDEEKARAKERFFSHRDEFGQWDPK
                     NQRPELWNLFNGRKHIGEHFRVFPISNWTEMDVWQYIYQENIPLPRLYFAHDREVIER
                     DGAFISTHPEWVKLRPNETAKTMHVRFRTCGDLPITGAIESDADTVEKIIYEVATTRV
                     TERGNRADDKRSETSMEDRKKAGYF"
     gene            2925..4223
                     /gene="cysN"
                     /locus_tag="PLL28_00340"
     CDS             2925..4223
                     /gene="cysN"
                     /locus_tag="PLL28_00340"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HNF67785.1"
                     /translation="MEHLVQDGKFDHHGYLNMELLRFTTAGSVDDGKSTLIGRLLYDS
                     KSIFQDQLEQIEQTSKNRGEEYVNLALLTDGLRSEREQGITIDVAYRYFSTPKRKFII
                     ADTPGHIQYTRNMVTGASTANLAIILVDARNGVVEQTYRHSFIASLLQIPHVVICINK
                     MDLVDFSQEKYEEVVEQFRHFASKLDVQDVRFIPISALLGDNVVDRSKNMPWYEGPTL
                     MYTLETVHIGSDHNLIDCRFPVQYVVRPMSNEYHDYRGYAGRVAGGVLKPGDEVMHLP
                     SGITTKIAEIKSLDGDLEEAYPPQSVTLILEDDVDISRGDMLVRENNAPKTGQDIDIM
                     LCWMDDKKSLQLNGKYAIKHTTRDARCVVKEILYKVDVNTLHRIQDVDSLALNEIGRV
                     KIRTTVPLFYDEYRRNRNTGSIIIIDEATNTTVGAGMIIE"
     gene            complement(4494..7565)
                     /locus_tag="PLL28_00345"
     CDS             complement(4494..7565)
                     /locus_tag="PLL28_00345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNF67786.1"
                     /translation="MMKAFTISILLMIVTAGLSAQTTLVGNTTFDFQSYWANHNRLLV
                     YPDGKVSTAWVGSDGFGAAFNDRGMFYNHFNGTSWGAFPSGRLEDQKTYFGELLKVMG
                     HEVVISDETTRLLVNKNSTVGATDWTETAGSNVIDGYNPMAFCPEGTDDIYVVNTKKI
                     TYESLLFSRSDDGGETWAVNEYFLPFTEEAYGIELVNPDCYQIVVHGNDVYVLYGANF
                     CDLILMHSPSKGDPGTWTSQTLVNFPIDNYTGAAGQTSDYDGDGDKDTIYTTDGMHEM
                     IITDDGTVHVFSGRMRIYDPTTAAGYYYIPKTSGIYYWHTGMASATVLDVMVDWNNDD
                     GLDDPYAGLGSDFTAYYAESVTTMPTACYDPSSGRVYLMFMMPVEYEISAGNQTYYDI
                     FGCYSDDGGTTWTSPVNITYTAYLGHENAYASAFPKVMDGKIHLQWQQDEEPGTTQDS
                     PADDIVINNILYNAFDPSRFDPYNPTVDFTYTLIPVGMTYSATFTNLSVDAEEYYWEF
                     GDGGTSTLENPTHIYAPGIYNVCLTGYNVYGETTVCEEIIAMNEPVADFTYIGDPEVA
                     FIDLSTGSPAEWYWDFGDGFTSTESDPVHTFSDNGVYNVCLTVTNGAGSDTHCENVTI
                     DSYVAPTAYWIYSGDPTVTFTDLSIGSPTSWSWDFDDGFTSAIASPVHTYAVNGTYHV
                     CLTVSNGAGTSTSCQDVVIDSYLSPVVDFSFTGDPTVSFTDLSTNSPTSWDWDFDDGT
                     FSTVENPVHTFPTNGSYNVCLTATNGIGSGTDCKVVVINGYAAPVALFSYSGDPVVSF
                     TDLSTGTPTSWFWDFDDGAFSTVENPVHTYATDGIYSVCLTVTGPGGVNTGCQDVTIS
                     STAGAPTAAFSYINVGPLTIKFTDLSTNAPTDWSWDFGDGSISGLQNPSHTYANIGEY
                     NVCLTASNGIGEDTYCEVIFAGDDIQSLDINHVIAYPNPASSILNVNIPNVSGDHDVS
                     IINMLGQKLPVKELQFIENILTLNIAELPAGNYMVLVHQDGGNYITYFTKE"
     gene            complement(8009..11281)
                     /locus_tag="PLL28_00350"
     CDS             complement(8009..11281)
                     /locus_tag="PLL28_00350"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNF67787.1"
                     /translation="MKKRTLKLLTLSLILGISSCLTAQTFTIGADNGMPGPTNYPSPF
                     GDYYKTMRAQYLYRASELTDMGMSAGFITEIAWNVAALPGTIDGTEDYTIKLLNSGTT
                     SLGLTTWEEGATTVWGPTFYNPVLGINTFTLASPFFWDGVSSIIVEVCGGDFNATFTK
                     NARVTWTGPLAFNGSHTRQSDTEPDPCGYTGTEYWENSPGGPDYRPQVIFTTIPGTDC
                     AVVPSLGVTNTTATNVCIGEEFTLSVPPVAELGITYTWYSSPDGAAYSLIPGAVSSSL
                     TTTQIATTLYRCKVTCTISGDFEVSSPVTVNQNDPTSCYCIPTYTTGTTEGDYISHAV
                     LGTLNNTTGALPAPFYNYYTALSTDLMAGTSNTLSVTVGTYATQNGFAAWIDYNHNGV
                     FEASEKLGEVTNLAAMATGNIIFTVPVTAATGTTRLRVREVWNQIGILPCDEYGFGET
                     EDYNVNIIPGTIPTAAFTYAGDPTVTFTNTSTGLPTSYSWAFGDGGISTATNPAHLYA
                     TNGTYNVCLTATNLYGSNTACQNVVIDSYVPPVANFTFSGEPTVSFVDASLNSPTSWF
                     WNFGDGGTSTLENPTHTYTYNGTFNVCMTATNATGSNTSCQFVVIDYYLAPVAAFTYS
                     GDPAVSFTDASLNSPTSWFWNFGDGGTSTLENPSHLYASNGVYNVCLTATNATGSNTS
                     CQLITIDSYLSPVAAFTFTGDPTVTYTDLSTNSPTSWAWSFGDGGVSTLENPTHTFTT
                     NGTYNTCLTATNAVGSNTSCQDVIIDSYVYIPVADFTYTGDPDVEFTDISSNAPTTWA
                     WDFGDGGTSSLENPIHLYASDGTYDVCLTAGNIAGSDTECKTIIVTGYVAPSALFSFS
                     GDPVTTFTDLSTGSPNSWLWNFGDGGVSTLENPSHVYATNGIFNVCLSIVSPGGSDAY
                     CTDVTIAGNGSAPTIDFSYVINSPTVAFTDLSSDSPSDWLWDFGDGAISGLQNPTHAY
                     AVEAVYEVCLTATNDFGTNTDCKSINVTDNAIQVLDQITLNCYPNPAGTYTMIEGLPA
                     GTLSGDIHITSVTGQEVGDNSIISWDNGICIINVSKLAAGTYTVKMLVDNQLFTGQFV
                     KL"
     gene            complement(11480..14566)
                     /locus_tag="PLL28_00355"
     CDS             complement(11480..14566)
                     /locus_tag="PLL28_00355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNF67788.1"
                     /translation="MKKIYSLMLGVVAWGAVLAQPVTIGTTTYDLQTNSANKSRLHVY
                     DDGKVSALWTGSTSLAATFSDRGMFFNKYNGTTWGGFPTARIEGVRTGFGEIINVGDH
                     EIVLSHDGTNIRVYKNATIGGTSWTETAGSTDITGLWPNAYCPAGTNDVYVVNANASS
                     PTQIYFSRSTDGGENWEVLEYVLPYLTTAEGFGAITAEAYQIAVYGSDVYVLYGASYT
                     DLVLLHSDANGDPGSWTSEIIWDNPLENYTGLVGEDTDYDTDGDYDTILTTDGNIEML
                     ITDDGTLEVFAPAYYMLDDDPTTAGWSYFPTISGFLYWKTGLVGMYYLDVLVDWKNDD
                     GLDDPFAGIGQQFIAYGGESFTSMPSAAYDPATGRLYLTYTMPVEYTDQFGDPTLPTS
                     ESKCDIFGTYSEDGGESWSYPVNLTYSAFKGKENYFPMVYDRVVDGKIHMIWQQDDNP
                     GTAVDVPADPIHTNYILYSDWDPSRFDPYAPTVDYSYVLTAVGATFNATFTNLSTDAE
                     TYFWDFGDGGTSTLTNPTHTFAEGVYDVCLTGYNVYGEATVCQEIIAVVPPVALFDYS
                     GDPTVTFTDLSTDEPTSWYWDFDDGATSTEENPVHTYAFNGIYNVCLTVTNAGGTNTA
                     CENVIISSYAAPLANFSFTGDPTVSFTDLTGGDPTSWSWTFDDGGVSTDQNPVHTYAT
                     NGTYNVCLTATNALGSSTSCQDVIIDSYLPPTVMFSYTGDPTVSFTDLSTEDPTSWSW
                     DFGDGSFSTEQNPVYMYAANGTYNVCLTASNAVGSTTGCEAVVISSYVAPDAMFTYSG
                     DPDVIFTNLSVNSTSWFWNFDDGSFSSEENPVHTYTSNGTYNVCLESTGPGGVDVYCE
                     DVVIGSVVTAPVADFNFFFGVGGSVSFFDNSSNSPTTWAWDFGDGGTSTEQNPVHVYT
                     AGGSFTVCLEASNAGGGDDVCKVVDVTAIAGLNVTNLNVWPNPAEGAISIALPAITGD
                     VQVAIFNTVGQKVVNSNIQAIQNQTLTINIGELASGAYTLYMTNGNAAYIGQFVKE"
     gene            14652..15110
                     /locus_tag="PLL28_00360"
     CDS             14652..15110
                     /locus_tag="PLL28_00360"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNF67789.1"
                     /translation="MKNTLRFPIAALAFLVLPLIATAQNGTPEAGRQDSLPVMDTTDH
                     TPNMIYEYLETMPEFPGGTMALYEFINTNLQYPEAAKKKKIEGNVYAKFVVTEDGSII
                     NIAILRDIGGGCGEEVVRILSTMPKWKPGMQNGKPAAVFYKLPIKFTLSD"
     gene            complement(15126..16457)
                     /locus_tag="PLL28_00365"
     CDS             complement(15126..16457)
                     /locus_tag="PLL28_00365"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNF67790.1"
                     /translation="MSFPPGFIANLQHNIGFDKAAFVASHDEVAPVSIRYNPLKSAKR
                     LSDDPVPWCENACYLAERPLFVYDPLFHAGAYYVQEASSMFLDFILRKLDADKKPLRI
                     LDLCAAPGGKSTLIAGAMHEESILFSNEVIKTRAPILRENLTKWGKENVVVLSQDPAT
                     FTGMQEYFDIIVVDAPCSGSGLFRKEPAAIAEWSEENVITCSKRQQRILTDILPALKP
                     GGFLIYATCSYSMEEDEHVVSHFIEQGLTNRNDIISSIETQGIISTALGYRFYPDKIR
                     GEGFFISILQKNNAEELAISKGKQLNYQLASSEEREIIRPWLKNFDKFIFVKHNNVFF
                     AWTKQLQEALKAASGLNILQAGIAIGEIKQGSLIPHHALAMSNIISDNIPRIALSKSE
                     ALEYLRKNNIELNSSEPGWHIITYENIALGWVKMIPGRHNNYYPTEWRLRK"
     gene            16582..20577
                     /locus_tag="PLL28_00370"
     CDS             16582..20577
                     /locus_tag="PLL28_00370"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNF67791.1"
                     /translation="MFTRTILLIGLLGSFCFAKAQLVINEIATNGAVLDAYNEESDWL
                     ELYNNSATAIDLNGYALTDDPLFPNKWPLPAIMLDANEHLLLLANGNNTYALIDHWES
                     AVLNETERTYFVGTSEPPSDWNTVGFDDTGWSTGPGGIGFGDGDDNTVIASATSCYMR
                     CAFDVADVSVIENAKLHMDYDDAFVAYLNGVEIARSGNIIGTPPTYNTLANADHEAGM
                     YAGYPAEEWNIPAVVMNILLVPGENILSIQVHNVTAGSSDLSSNAWFSVGINNGLTYF
                     SPVPAWFAEPVVYSETNFKITNTGETIYLFDNTGAIADMVNPGSVAYGHAYARIPDGA
                     ASWCTTTTPTPDNSNDGAPCFAGYEPLPVFSLAAGFYTGTQTLTLSSTSPTAQIRYTT
                     DGSLVTATSPVYTGPITISSNEVVAARCYSTGTLLTGEQVKRSYFIDEDDYTLPILSI
                     SIDPGSLFDYDTGIYEFGCCYDAAYPYFGANFWQPWERFGHIEYFTADGDFEWGKDMS
                     LEIHGGWSRAEAQRGFRVDFKNKYGGNLEYPLFGGKPDASSINNFNIRSGGQHVWTYK
                     IQDAFLAKVMEDTHIDYEEWQPCMLFINGDAWGLYEIREKADEHFVESNYGINNNDVD
                     LLNSYSVLNGSDTGWWNIYSSLMSLDPLSDIFYDKFNQAFDMQNYIDYYIGEIYYQNV
                     DFGGYYWGANNMKVWRNRYGGKWRFIMYDMDGAMGYFGSSPYDNYINLTRSPAYPSSY
                     SQIFDRVLDNQEIREYFVNRFADLINTIYQPDNMEAVLYEMRDSILSEIPHQVERWGA
                     PNTATVNSYVDGLLSYNNSRKNAARTHINNSFDLDGQRTTTLAVSPAGAGYIKINTIV
                     PTELPWTGIYFDGVPITITAVPNPGYTFDSWLPNALLPGGSTDISLTINMDNSTTFTA
                     VFTGTAVTPEIIVSEINYNSYVDYNMGDWIELHNAGTDAIDISYWKITDRYEGNAYIL
                     PAGSVIESDEYLVIAKDPIAFSNVHPDITNVVGGYDFEWDNSGDDIRLFDIAGNEIIH
                     MAYADSLGWPQGADGTGRTLELINPTADMNNPGSWFDGCIAGSPGEAYSPCNDALVFS
                     EINYNSSAAMNAGDWVEILNTTADDMDISGWKFLDGEDSLIYTFESGTTIPANERLVL
                     SGDLDLFESRHPDITNVTGAFNFGLDASGEEIRLFNDHGILQFTVIYDDAAPWPYEAD
                     GAGKTLELESASGIMNNGDNWFAGCPEGSPGTIYDPDCDGVIAIEESVPASLLVFPNP
                     AKNYVMIAFENNYNSGRIELCNLSGQIIYETQSLENGQVIIPRNEIASGVYRVRLIAD
                     KKMIESLLVWE"
     gene            complement(20587..21558)
                     /locus_tag="PLL28_00375"
     CDS             complement(20587..21558)
                     /locus_tag="PLL28_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNF67792.1"
                     /translation="MADIASIQQPIHEELALFEEKFRASMRSNVALLDRVMYYIVKRK
                     GKQMRPMFVLLSAKMIGNISESSYTAATLIELLHTATLVHDDVVDDAYERRGFFSINA
                     LWKNKIAVLIGDYLLAKGLLVSLDNNEFRMLKITSDAVKEMSEGELLQIEKARRLDIK
                     EDVYFEIIRKKTATLIASACSAGAASVTTDEDTISLMRLFGEKIGIAFQIKDDLLDYG
                     TDDIGKPLGIDIKERKLTLPIIYTLLHAPKNDKSEIMRIIKHYNTDKEKVNWVIDYVK
                     KGGGIAYATEKMKQYQQEAFELLEKFPESESKRALKDLVIFTTERKK"
     gene            21664..22476
                     /locus_tag="PLL28_00380"
     CDS             21664..22476
                     /locus_tag="PLL28_00380"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNF67793.1"
                     /translation="MFKVNRTVVIVSCFGMFLSGIIAMQSCSEGGGINIFSIEDDKAL
                     GDQIAAEIAGDPVNYPILDSVDYADAYAFVYDIRDRILESGMVFHKDDFKWQVRIIDQ
                     DSVINAFCTPGGYIYVYTGLIRYLENEAQLAGVLGHEMAHADLRHSTDQLTQAYGISI
                     LLDIVLGDDQNAITDIAASLTQLAFSRGDESQADEYSVIYLCPTEYEASGAAGFFEKI
                     EAEGDIQIPEFLSTHPNPDNRIDAIYAKSSELSCSGDQTYDARYQDLIDALP"
     gene            complement(22554..24740)
                     /locus_tag="PLL28_00385"
     CDS             complement(22554..24740)
                     /locus_tag="PLL28_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF67794.1"
                     /translation="MARYFLFLTIVCISAALSAQTAETDTSKLKLLPTVNVQAERTIP
                     DIQRIQPVKGTFIYSGKKTEVIDLTQRTVSLSEKYGRQIFSKIPGVFVYDMDGTGNQI
                     NISTRGLDPHRGWEFNIRKDGVLTNSDMYGYPASHYNIPMEAVEKIELVRGTGSLQYG
                     AQFGGMLNYISKQPDSTRALAFETSNTVGSYGLVSTYNGASGKIGKFRYSTWYNKKWI
                     TGYRDNSDAQYDAEAVSLFYDYSKDLTFKFEWTHSNYITHLPGPLTDSMFYADPQMAT
                     RSRNYYNPNIHVPSINMNWNITSSTKLQLTSSAVIGARNSVMFDRPATTEDTIVGATL
                     DYNNRQVDIDHFNSYTNELRLLQSYKLLGHTSNLAAGVQYMYNDLHRQQIGKGTTGSD
                     FDLTLAIPGWGRDLHFITSNIAVFAENRWVLSDRFSVNTGARIEMGESNMSGVITYYP
                     DSTLPNTIKHSFPLFGVSAQYNVSKFTNIYGGWSQAYRPVIFKDIVPASVYEYADKDL
                     KDAYGYNAEVGYRGNWKFLKWDVTAFYLAYNNRLGTLAETDTAGNLIIRRTNIGDSRT
                     TGLEFFMQGDFDLTRKLSLSIFTSTSYMDARYHDATIRSGSENIDIDGNKVESVPDII
                     SRNGLTLKYTRYSLSALYSYVGESYADALNTVEPNASGSVGLVPSYQLIDINIGVKLT
                     NQVKVQFNANNILDEQYFTKRPQFYPGPGIWPSDGRTFSGTVVVKL"
     gene            24914..26536
                     /locus_tag="PLL28_00390"
     CDS             24914..26536
                     /locus_tag="PLL28_00390"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNF67795.1"
                     /translation="MKRLFSLLILGSVLTMNGQTIPSKPKLVVGIVVDQMAYEFLYRY
                     SDTYSDSGFKRLLRDGYSCENTHYNYIPTYTGPGHASIYTGSVPALNGVVSNDWFNEQ
                     SGAYVYVTDDSTVSMVGGNGLGSKMSPKNMVATTITDALQLSNQQRSKVIGIALKDRG
                     AILPAGHAADAAYWYDGASNAWVTSTYYMQQLPTWVSAFNMQQPAQKMMNKNWDLLLP
                     ASQYNQSTADSVSWETLYTGETSPKFPHYLPFTGNSESIKGTPFGNTLTAQFAMAAIK
                     NEELGTGPETDFLCVSFSSTDYVGHAYGPHSMEIQDTYLRLDKDLGELLTYLDTQVGK
                     GNYLLFLTADHGVTPTPQYMESLRIPAGTTTEVSMLQSMQAMLNAAWGQKDWISAYTN
                     QQIYLNDALLQAAGKTIEDAYNVLYPQLMKMDGVSYVLQINKLNDATMNNDVKELVIN
                     GIYPKRCGDIQILYEPYWFEAYRPTGTTHGSHFSYDTHVPLIFYGWKIKSGALHRNVY
                     VTDIAATLADLLNISEPNACIGHVIPEVANGK"
     gene            26619..29153
                     /locus_tag="PLL28_00395"
     CDS             26619..29153
                     /locus_tag="PLL28_00395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF67796.1"
                     /translation="MAIVFILQKDDNNFHHTETNPAPVRVDGNHEKEDESYNRDRQNF
                     FDQMHKSAPGTNWRKMDADLRFQLMKQRSNNLRTDDIAMDTLASGALIGEWNEMGSYN
                     TSGRIRATEVDFENETIYAFSDGGNLWKGDLDGENWAVINDNMKITGAHTLRKIGDRL
                     IVGCDQWGVQGVFYTEDEGLTWNATTGLENVAAWGYISDMEILNDANHTIYLLAYEWD
                     YTNWWDIVSLYRSEDLGVSFTKLSSYDVPVYGGANHFVLWSPPYGSPDCYMIENTHVN
                     LLDATGTPVLQGTLPITDDGDYMLCGFEGPSSTSLYVAHRNYVDNFTHFYTSFDGGVN
                     WNTEGIIEEGNFSKNSFNCSQKQEGYLFYGGVDSYRSFTAGNSWVRNNYWYEYYGDIE
                     YNLHADIPFIRTFIDGGGDEIVLISTDGGLYKSTDNGLIWNNITNEGMRNAQYYDVYT
                     YRWVTDILFAGAQDQGYQRSSYTIGDDYYFDQLISGDYGHIVSRSGGDNLWCVYPGFA
                     MYVNDAASASNLTTWDFQGYGHLWMAPIMQDPWNDQVAWWGGGSDAGGAYLWRLQKSG
                     AFISGVKQAKNFSVAGGGSISAISYSPIDPNYWYLLTSGGNFYHSEDAGETWYLSSGF
                     SGPGPQYFYGATIEPSKSQLGVVYIGGSGYDNPAVYMSDDHGDNFVELQDGLPPTLVY
                     DLAVDQTDSLLFAATEVAPYVCVLAEGKWYNIAANDAPLQTYWSVDYVDEINTVRFGT
                     YGRGAWEFAIEKLPTINIHDANQEALMSVYPNPASEKIQITIPAYFSNAEVTVVNMQG
                     DIVYKTKATINKDMPYNIPVSGLAAGSYVIAVKQGEKMYTSKFVRI"
     gene            complement(29215..30789)
                     /locus_tag="PLL28_00400"
     CDS             complement(29215..30789)
                     /locus_tag="PLL28_00400"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HNF67797.1"
                     /translation="MKNKLLLLTSGILMFSGAFAQLTLTPEPNDTTFIYSLAGPGIVV
                     SNIERNCHTDASAFYNSTDANVGIENGILLSSGTAASSAGPNDVGAASSYLDYPGDTD
                     LEGLTMWLTYDACAISFDMTVMADTVKLKYVFASEEYPEFTFSGFNDIFAFWVSGPGI
                     PTPVNIATVPGTDLPVSINNINATTYSDYYIENGDGYMTPYSTDDYYIQFDGLTTVLE
                     AKLAVTAGETYHMKMAIADASDGIYDSGVFLETGSLGSLRMHTDVFADNDLTFAVEKC
                     ASGHFTFTNEIPSAEPLVIDYMIGGSAINGVDYETIPEQLTIPAWTSEGTIDIVPIHD
                     VIDESFESVILYLYNPQSGVIYDTVTMLIDDEPAAASFASTISDLSVAFTETSGAAVS
                     QYWEFGDGTISTESNPTHIYATAGVYNVCVTGYNAYGCSDTYCDMVTVGNVDINDLQN
                     TLSISPNPVANVLQLEGTENNAVINILNLQGEVVLTSTAYGNSVSIDVSALPAGIYTL
                     RATQGSQMAISNFEKL"
     gene            30860..31567
                     /locus_tag="PLL28_00405"
     CDS             30860..31567
                     /locus_tag="PLL28_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNF67798.1"
                     /translation="MSSFNFKQFTILQQDSAMKVGVDGVLLGAWAFSKGKVEHILDIG
                     VGTGLISIMMAQRFPEALITGVEIDEAACFEAIENAKRSPWSNRIKIINSDFLQLDEK
                     SSAYDLIISNPPYYEKGWPVEDEGRRKARDAASLPLVDLFQRAAKISGQHTVFCLILP
                     ALALDTCLQTAAINGWFSNRITTVLTKPEKPAKRVLIAFGRSESGTSTDVLTIYDANG
                     NYSEAYKKLTGDFYLAF"
     gene            complement(31633..32136)
                     /locus_tag="PLL28_00410"
     CDS             complement(31633..32136)
                     /locus_tag="PLL28_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67799.1"
                     /translation="MPKKKPLEGKPEVHKDLDGFEMNINEFGEITSNTDIDKLNQFLN
                     KNVDDKKFRGLDNLPYAKGGDDDEESTEEKDDLNEISDEFEEFRQFNDKDFKDEFDFD
                     EKDEADGYFNEGKHEYEQFEDEEDDAKADDEEDDFDDEEFLDDYADDYGEEDNYNLDE
                     GDFDDKY"
     gene            complement(32152..33675)
                     /gene="gltX"
                     /locus_tag="PLL28_00415"
     CDS             complement(32152..33675)
                     /gene="gltX"
                     /locus_tag="PLL28_00415"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNF67800.1"
                     /translation="MDRPIRLRYAPSPTGAQHLGGIRTALYNYLFVKKLGGKLILRIE
                     DTDQTRYVPGAEEYVINSCAWAGITFDEGVHIGGPFAPYRQSERKDMYRKYAMELIEK
                     GHAYYAFDTPEELEAMRNRMMEQGNPSPAYDHITRQYMKNSVALSKDETERRIQNGDP
                     YVIRIKMPRNEEVKFHDLIRGWVSFNSSQLDDKVLFKSDGMPTYHLANVVDDYLMQVT
                     HVVRGEEWLPSAPTHVMTYRMFGWEEVMPSFAHLPLILKPEGNGKLSKRDGDKYGFPL
                     YSLDWKEQNISGLKEKGFLPEAYINFLAFLGWNPGTDQEVFTEEELIAAFSFERIGKS
                     GARFDPEKAKWFNQQHMRRKTGSELADLLQPYLDKKGIQADRNYLERVCELMKERAVF
                     IPDLYEAGTFFFGEISEYDQDTIRKKWKPEKQEIFLNLIAHIESISNFTEPSIESGVK
                     DYMKENNLGMGEVMPLLRVALAGVLQGPPVFAMMDVLGKNISVQRLHTAIKIFDNFR"
     gene            33786..34433
                     /locus_tag="PLL28_00420"
     CDS             33786..34433
                     /locus_tag="PLL28_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNF67801.1"
                     /translation="MYFVKTPWYLKSVYPSLVWDMPKDNRLFLTFDDGPVPGVTDKVL
                     GILSKYDVKATFFCIGDNVRKHPELYQQILQEGHMTGNHTFHHVNGWKTDTDAYIEEV
                     SACSNLVNSQLFRPPYGRIGYQQIQELKRQYAIIMWDVLSGDFDTQLKPQDCIDHVIQ
                     HAADGSIIVFHDSEKAADRVLPALPVVIEHFLEKGYRFESLPANTASLPRGLHLS"
     gene            34452..>35170
                     /locus_tag="PLL28_00425"
     CDS             34452..>35170
                     /locus_tag="PLL28_00425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNF67802.1"
                     /translation="MPELIDTHTHIYLPEFDSDSDDMIQRALQSGVSHMMMPNIDLES
                     VDAMMALAKKNRACKPMMGLHPCSVKNDADDVMLKIHQLLLDKQYQWYGIGECGLDYY
                     WDLTFVDQQKAAFVTQIRWAKEMQLPLIIHSRNATDDCIDLVSKHKDANLRGIFHCFS
                     GTELQLQQSIDLGFYIGIGGVVTYKNGGLDKVLKPEHLPHLVLETDAPYLSPVPFRGK
                     RNEPSYLVQIVNKLAEILGLPV"
     assembly_gap    35171..35270
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<35271..35459)
                     /locus_tag="PLL28_00430"
     CDS             complement(<35271..35459)
                     /locus_tag="PLL28_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67803.1"
                     /translation="MAQHFEIKNADERSEWLHGIYLKPPISRDHANNPSSLLPIKISI
                     ERSEWLHGIYLKPPSQGIM"
     gene            complement(35524..35614)
                     /locus_tag="PLL28_00435"
     tRNA            complement(35524..35614)
                     /locus_tag="PLL28_00435"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35578..35580),aa:Ser,seq:gga)
     gene            complement(35671..36960)
                     /locus_tag="PLL28_00440"
     CDS             complement(35671..36960)
                     /locus_tag="PLL28_00440"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNF67804.1"
                     /translation="MTRYIKQLLTIAFLLHIVATHAQQVPVAISGGRIHVGDGTVIEN
                     GLVIIQNGSIQYAGPMKESGYDANTASLIDATGKDIYPGFIGMGSTIGLNEITAVRST
                     RDAYEVGVYNPNIRSLIAYNTDSKVIPTVRTNGVLLAQVVPYGGVIGGQSSVFKMSGW
                     NWEDAAIATDDNMHMSWPSVYNYDYEKNTYGPNAQYRSEVEDIYTFFAEARSYCNGNA
                     PREHNLRFEAMRKVLNGKQKLFISANSAKQIIGAVALSDSFHIKIVIRGGRQSYRVTE
                     LLKERNIPVLLEATHELPSYDGDPVDLPYKLPAMLHDAGIMCGLSVCNEGDSYWNLRN
                     LPFQAGTAAAYGLNREEALKMITLNNAMLAGIDEKYGTIKTGKSATLFISDGDALDMG
                     TNRLSIMFIAGERLKPDNWQSELSNKYELKYNIQIHR"
     gene            complement(36982..39939)
                     /locus_tag="PLL28_00445"
     CDS             complement(36982..39939)
                     /locus_tag="PLL28_00445"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNF67805.1"
                     /translation="MQKILLTALSILGASIIYGQTTFPVNGVYDERSRTYAFTHATIV
                     TDPGNTLQDATLLISDGRIIDVGTAVVIPKNAVVEDLHGKYIYPAFIDLDAGYGMPAK
                     ENPQVGRGPQMLNSNQQAVSWNQAITPEYQAVSGFKPQGDVAKLWRSSGFGLVLTHRH
                     DGIMRGTGALVSLGDERAGEMVVKPNISDHYSFRKGTSTQEYPQSEMGSIALIRQTFY
                     DADWYGRVGKNSEQNISLQAVLDQKNLIHFFDTDEKLEILRVAKIGKEFGYNFIMMGS
                     GNEYQRINEIKQTGAVLVLPLNFPKTPDVSDPWDAELLSISDLKHWEMAPANPAITEQ
                     AGITFALTSSGLDDKSQFLPNVRKALQYGLSEKTALSALTSVPAQILKLESETGSLAK
                     GKRADFFISSDNIFREGAIIYTNWVNGLPYVVNSENSDLRGAYALHIDNMVYGLLIQG
                     KINAPEFSIIKNTDTLKAQGAISTENIQIQFADNGTLYRLTGYHDGKNIEGQGNANDK
                     WINWKAEFASVYAEKPTPQKKDTVQIGTVMYPFTAYGWEAAPTQGNILITNATVWTSE
                     KEGVLKNTDVLIGNGKILQVGKNISAKNARIIDGTGKHVTPGIIDEHSHIAVTDGVNE
                     GTQNSSAEVRIADVINCDDINIYRQLSGGVTSAHILHGSANPVGGQTQLIKLRWGAAP
                     EDMKFAQWPGFIKFALGENVKQSNWGDDYSVRFPQTRMGVEQVYVDIFTRASEYAKAK
                     KENPQSVRTDLELEAIAEILEGKRFITCHSYVQSEINMLMHVADSFGFTINTFTHILE
                     GYKVADKMKIHGAGASTFSDWWAYKFEVYEAIPQNPGIMHDAGLVTAINSDDAEMGRR
                     LNQEAAKSVKYFNMSEPEALKMVTINPAKLLHVDDKVGSIKVGKDADIVIWSEDPLSV
                     YAMAEKTIVDGKILYDRSIQDQLIAAEDAERHRIINKMMAEKTNGAITVPIVVTPHRE
                     YDCEDVIDFMKGE"
     gene            40148..42490
                     /locus_tag="PLL28_00450"
     CDS             40148..42490
                     /locus_tag="PLL28_00450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNF67806.1"
                     /translation="MFRKYLPLILALALCNKVFAFHIIGGEMIYKRLTGNDFEITLKI
                     YRDCSNPEAAPFDDPLQIYVYNASGILVDSLVLFFPGSDNIDPEIVNPCMTVAPDLCV
                     QEAIYVGNINLPPSIGGYDLVYQRCCRNSTIINIYDPIQTGTTYTEHIPDPGTLINSS
                     PEFNNLPPIALCAGYPFAFSHAATDPDGDALVYKFFTPYNGASYDLPDPAPASPPPFN
                     HVIFVPPFNESYQIASSPAFTLGSTTGWLAGTPSDLGQFVVGVAVEEYRGGVLIGTHY
                     RDFQFNVTDCTPDIVASAPPEINNCTDFTVLFDNESIGTDDFYWDFGVPGVTDDNSTE
                     DNPVYTYPDTGTYTVMLIAFPGTDCSDTTFTTVHIYPQLIPGISFENTCAGSPVNFMD
                     ISTTDFGTITSWQWNYGDGWGSSEQNPVYSYEEPGNYMLIFTVENSVGCIAEIYDTIT
                     IYHLPFADVATDSACLHTSGTVFDASVVLAGNDIVDWQWEMEPDQYFSGESFDYYFDT
                     AGVYLINLTVTTDKGCVDSILDTIIVNPQVVAGLLNDTVICEGDSVQVFASGGTYYAW
                     EPPSGVSDPSSPEPWLSPDESTVYGVIVTDGCTSDTAFMQVEILPAPHVIAGPDTIVY
                     HGQEVAMYANGASSFNWEPPDGLSDPFSPNPIALPDVTTLYLVTGTAENGCISTDTAL
                     VYIIPNCFNFATVNAFSPNDDGVNDRFRFIIAGDDGLVSMEIYNRWGKMIFNTNDIEA
                     GWDGTDGNGHPQELGSYIYVIYTSCDGKEQRLSGSVTLLR"
     gene            42531..44687
                     /locus_tag="PLL28_00455"
     CDS             42531..44687
                     /locus_tag="PLL28_00455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNF67807.1"
                     /translation="MKRTRLLLLSLMTIIGLKAHASHIIGGEISYTCIGTNQYQVVLI
                     LYRDCTSFTPFDNPAYMTIYTQDGDYVNNFPLYSPFITGIPAESDNPCLDAPDDVCVE
                     QAIYTAIITVPDGDQGYLLVFQRCCRNAGIVNIETPDDIGATYTQEIPPQTDATCNTS
                     PVFNAFPPTVICIDDPIVFDHSATDIDGDSLAYDFYWPLQGASSFDPAPVIAAPPPYD
                     GVVWVTGYDELYPLDAVPAFAIDPVTGILTGTATAEGRYVVGVRCKEYRDGVWLGDHI
                     RDFQFNVVTCTPTVVAQMDVGDATFDDTTPDDEYLNCSDFTVIFDNYSIGGTEFFWDF
                     GDGSTSTLEDPTHVYADTGTYTVMLIVNPGFVCADTAIGIIKLYNTLTAAYDYSAGCS
                     GTPVTFEDNSNSTEAGDIITWDWNFGDGATSTTANPEHEYADGGTYTVVLTVTTDVGC
                     QSSVSVDILVEPGPDVDFLAPDVCQDEEAEFTNLTTITTGSISSYSWDFGDGATSTED
                     EPSHQYDAPGTYTVTLIAYSANGCSDTLEQDIYIGMLPDAYAGVDDTIMYLETYTLNG
                     TGTGDFVWDPQNPIVPPASISSLLVANPTIELAETTTFVLTVTSPDGCSQTDEVTIYV
                     EDYPIVEVPNAFSPNGDGTNDVISAFLHDISELEQFTIFNRWGQVVYTSDIINDAWDG
                     MIDGKEAEMGTYVYLVVAKDTNGDMITRQGNITLVR"
     gene            44743..45069
                     /locus_tag="PLL28_00460"
     CDS             44743..45069
                     /locus_tag="PLL28_00460"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNF67808.1"
                     /translation="MKKLLIFFAVAATIASCTTASSSDQDTATAEPTASPGMLVYTKH
                     CKLCHGAKGDLGLSGAANLSITLLSHDEITHVVTEGRKGMPSWKAQLSPQEIQEVTDY
                     VLTLKK"
     gene            45069..46184
                     /locus_tag="PLL28_00465"
     CDS             45069..46184
                     /locus_tag="PLL28_00465"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNF67809.1"
                     /translation="MSPERKTYTAIGVMSGSSLDGVDIALCEIKLEAQSSFEIIAAET
                     YKYANQEISYIKSLLPVITDDHHAAHTFFAEIFARHITQFLKLFPDRQIDFIALHGHT
                     LLHFPAQGVSLQLGDPQLMANILGIPVIAHFRQADIDAGGQGAPLVPMCDVIFFKQYD
                     ACLNLGGIANISFESASGRIGFDVCACNQLLNYCAAMAGLDYDPEGELAAAGMIDQDL
                     LDQLNAVPYLDMPWPKSLDNHFVHRAFIDTIAKSTIPLNDKLATAVEHIAIAIAAVII
                     AQPERRPLHQYQLLVTGGGAYNTFLIERIAANAGIKITLPGDKIIQYKEALAMALMGA
                     LRIQSKPNFLQSVTGAKTAVSGGVIAYPEKIIAPLPS"
     gene            46181..47188
                     /locus_tag="PLL28_00470"
     CDS             46181..47188
                     /locus_tag="PLL28_00470"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNF67810.1"
                     /translation="MIVVTGANGLVGSYLCRWLLQQGKQVRGMRRADSNMRLVEDIAS
                     QIEWVIGDVTDVVAIENAMSGVDQVYHCAAVISYRKKSAAQMMRINGEGTANMVNIAL
                     DLGIKKFVHISSIAALGRTGKDGEIITEEAPWDMKYITTDYAISKFTAEREVWRAMAE
                     GLKAVIINPSIILGAGDWENGSSKLFSTIYKGFKFYTTGITGYVDVRDVVRIAQVAMD
                     SDITEQRFIVNGDNWTNKDVLQHMARSLHVKEPSVHAGKTLSEIAWRMEWLKGLFSGK
                     EPAVTKQTARIANATTYFNSNKMKDTFHYSCVPLEKTIADTAAAFLAEKTDGKFHPLE
                     F"
     gene            47190..47882
                     /locus_tag="PLL28_00475"
     CDS             47190..47882
                     /locus_tag="PLL28_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HNF67811.1"
                     /translation="MQNIHHILFDLDNTLWDFSGNSKRILAQIHNDLSLDQYGIPDFH
                     RFHEQYAFRNEYLWHQYALGNVTRDEVRLNRFYITFNDFGLDNYVLANKAADFYIHHT
                     RKQTDLLPYTRELLDHLHGKYGLHIITNGFDEVQFFKLENTGIRHYFQTVTTAENAQS
                     LKPNKKIFDHALSLIPAKASECLYIGDSPDVDGKGSIDAGMSFIWFNPAKKENKEGYR
                     EVQHLSEIAALL"
     gene            complement(47947..48351)
                     /locus_tag="PLL28_00480"
     CDS             complement(47947..48351)
                     /locus_tag="PLL28_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67812.1"
                     /translation="MKKLLSFALVTAFGVTVAMAQTATTATTTTTVTATEQVTTAAPV
                     IKAVEAEQGNIKCTGTGSCCSHGVSRSGATTTATSEGAGVSTEVTTTGEAAAVETTGE
                     IKATDAGSCHQLSIGVSAAKPEPAEKTEDPKK"
     gene            complement(48391..49074)
                     /locus_tag="PLL28_00485"
     CDS             complement(48391..49074)
                     /locus_tag="PLL28_00485"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HNF67813.1"
                     /translation="MKFTRFIHKEGRKIIIGFLVFLVILNFCTHYFMPEARLFNTLVL
                     IASLGGIIFVMSFFRNPKRSLFIEEGSIIAPADGKVVVVEETYEGEYFKDRRLQVSIF
                     MSVKNVHVNRNPISGQVKYFKYHPGKYLLAWHPKSSTENERTTFVIEDEDDNVEILMR
                     QIAGTVARRIKFYVDEGDAVTQGSEFGFIKFGSRVDLFLPLDSKVEVKIGEKVKGGQT
                     VLARVMRHN"
     gene            complement(49142..49972)
                     /locus_tag="PLL28_00490"
     CDS             complement(49142..49972)
                     /locus_tag="PLL28_00490"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNF67814.1"
                     /translation="MNNLVLRIITALVGVVIIIGSIFVDRTAFYAVFMLIGFLTHLEY
                     VRTTSATPARGKWIVMGLPLAFGIMIMTLQTGIINGLLYNYIQNDVLIELLLLIIPIT
                     LIVELYLKREFPFQQVGLTVLGLLYVVFPFGLYMNYGAGNFQQAFALGLLCLVWSNDT
                     FAYFAGRLFGKNKLFESISPKKTWEGFIGGLLCTMACGFVLSRFYPEYISATQWIVLG
                     LVVSVAGTFGDLVESMLKRSVQIKDSGSLLPGHGGFLDRFDGFMFAVPAAVFYIDFIR
                     "
     gene            complement(49969..50184)
                     /locus_tag="PLL28_00495"
     CDS             complement(49969..50184)
                     /locus_tag="PLL28_00495"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNF67815.1"
                     /translation="MEKDWMQVYSTYDRMEANYLMSELQQHAIYAVLFEKPPMPFGGD
                     VMPVKFELYVHDKQAEEALELIYNLPE"
     gene            complement(50117..>50312)
                     /locus_tag="PLL28_00500"
     CDS             complement(50117..>50312)
                     /locus_tag="PLL28_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67816.1"
                     /translation="AIFLTHRGVIQGDITESSNIWFGLIAAPFVFLLLYLYRNKESHG
                     KRLDAGVLDVRQDGGELSDE"
BASE COUNT        14329 a        10378 c        10524 g        14981 t
ORIGIN      
        1 aacagggtat tgcagatcag tagtggtcat atctttcgcg gtttattgaa atggcatttc
       61 agtgctagca atggaaccaa ttacagtcgg gtgatcatta tgtgctatag catcaaattt
      121 cggatcacgg ataactttgg tatacttatc gagatcccaa tcacttgata ctgctttgcc
      181 gatgtttgaa tgtataccga tataaataaa agttttatca ccgtcctccg gactaaattc
      241 actcaaatac ggacttaaaa tattgatggt gtcccccgtt tccactgaag tgactatggc
      301 atgtatatac gcgccatcat tatctacctg tgcgggacca ttgctataat acaccaattt
      361 aatttcggcc atatccttta gtgcgtcggg ttgcatatta aactcgctta attcccaata
      421 atcataacca ctattgcatg aaatgacaac acccagactg ccgataacta ctaagatagt
      481 ttggaggaat ttttgcatgg gaggcgattg gttgtttggt tgattaacgt gaatgatgca
      541 ggtagggttg tttgcagtat gtcatttttt acgcctgacc gcataaatta gttcatcttc
      601 aaaaatacca ttcttaaaaa ttgttttctc caggcgcgct tcgagcttga atccgttttt
      661 ctcaagcact ttttgtgaag ctttattact accgaaaggt cgggcgaaaa ggcgttcaat
      721 atcatatgtt ttgaaagcga aatcgatcat aagaggaatg gctttgctca aaataccgtt
      781 cctccagtaa tcttccgaca gccaatatcc caactctgca ttcttgcaat gaatatcggt
      841 ctgcgggtgc atgccgatcc caccgacagc atgaccttcc acctcgatag caaagatgtg
      901 gacagggtcg tcctgggtgg caaagtggat gaatttgatc ccgtcagctt tagtgtaggg
      961 gtgcggaaac ccattggtca tgttgcctgc tatatttggg ttattggcat gtttaaccag
     1021 atccggcagg tctttcatgt tccatgggcg gagtttaaag tccattatca aagatactca
     1081 gtttcaagat gttgcaggaa aatatgaaga tttgaggttt caatgagctt cggcttggtg
     1141 aaaaggtagc ttttatcaaa aaactcccgc catcccccta aaccgaggca gaaagtagac
     1201 ctatcgggag tggaataggc ttcccgggac tgaccattat cacccctcaa ggcctcgaaa
     1261 aaactgattt tttacgtcag attccttacc tttgcagcga tttttgcaca caatggcgcc
     1321 tgtacaggat aacatacatc cgatttttca ccgtttgctt cagcgttccg acaaggagca
     1381 gttgctgaaa cagaccgcga aagtgatatg gctgaccggt ttaagcggtt caggaaagag
     1441 cactatcgca gaggcattgg agaaggaact gcataaaatg gggtttctga ccatgttgct
     1501 ggatggcgat aatatcagga cagggattaa caataacctg agtttcagcg ctgaagacag
     1561 aatagagaac attcgtcgga tttcggaggt gagcaagctg tttctgaatt gcgggatcat
     1621 caccatcaat tgttttgtga gcccgacaaa tgagatcagg gcattggcga aaagcataat
     1681 tggcgaggcg gattttctgg aagtatatgt aaatgcaccg cttgaagttt gtgaagctag
     1741 agacgtgaaa ggcttatacg ccaaggctcg caaggggtta attcccgact tcaccggaat
     1801 aacggcacct tttgaggcac cggaacatcc tgctttggaa atacccactg ataaattaga
     1861 aattcaggag ggcgtgcaac gtttaatgga ttttatctta cctattatat cgaatcaata
     1921 atttaccatg agttatagct taacacattt aagggagctg gaggcggaga gcatatatgt
     1981 gatgcgcgaa gtagcggcac aatttcaaaa accggcttta ttattctccg gcggcaagga
     2041 ctctatagtg atggtttacc tggctcgcaa agccttttgg cctgctaaga tcccgttccc
     2101 actggtgcat atcgataccg gacataactt ccctgaaaca atggaatatc gcgactggtt
     2161 ggtgaaagaa atgggtgcaa cattaattgt tggatctgtt gaagattcca tcaacagagg
     2221 aactgcggtt gaagaaagag gtttgaatgc aagtcgcaat gcccttcaaa cggttacact
     2281 gctggagacc atcgagtcaa ataagtttga tgcatgtatg ggtggcgctc gtcgcgatga
     2341 agaaaaggct cgtgcaaaag agcgtttctt ctctcacaga gatgagttcg gtcaatggga
     2401 tccgaagaat cagcgtccgg agttatggaa cctgttcaat ggtcgcaaac atattggtga
     2461 gcacttccgc gtgttcccaa tttctaactg gacagagatg gatgtatggc aatatattta
     2521 tcaggaaaat attccacttc cgcgtttgta ttttgcgcat gatcgcgaag taattgaacg
     2581 cgacggagca tttatttcga ctcatcctga atgggttaaa ttgcgtccga acgagactgc
     2641 gaaaaccatg catgtgcgtt tccgtacttg tggcgacctt cctattacag gtgctattga
     2701 aagtgatgca gatacagtag aaaaaatcat ctacgaagtt gctacaactc gtgtaacaga
     2761 gcgcggaaat cgtgctgatg acaagcgcag tgagacttca atggaagaca ggaagaaggc
     2821 aggatacttc taaataaaat ccctgtgatt acgtatgaat aaaatagatt tattcgcaat
     2881 cacaaaaaaa gtaaacacat aatttaattt gtataatcat tacaatggaa catttagttc
     2941 aagacggaaa atttgatcac catggttatt taaacatgga gttactccgc tttactacag
     3001 caggaagtgt tgacgatgga aaatctactt tgataggccg cttgctgtat gattcaaagt
     3061 caatctttca ggatcagttg gagcagatcg agcagacaag taaaaatcgc ggtgaagaat
     3121 atgtaaatct tgccttactt accgacggtc ttcgaagtga gcgtgagcaa ggtattacga
     3181 ttgatgtagc ttatcgctat ttcagcaccc cgaaaaggaa attcatcatc gctgatactc
     3241 cgggacatat tcaatataca aggaatatgg ttacaggagc ttcaacggca aaccttgcaa
     3301 ttattcttgt tgatgcgcgc aatggtgttg ttgagcagac ttaccgacat tcatttattg
     3361 catcgttgtt gcaaatacct cacgttgtga tttgtatcaa caaaatggat cttgttgatt
     3421 ttagtcagga gaagtatgag gaggttgttg agcaattccg tcattttgca tcgaagttgg
     3481 atgtgcagga tgttcgattt attccaatca gtgcgctcct gggtgataat gttgtggatc
     3541 gcagtaaaaa catgccttgg tatgaaggtc cgacgttgat gtatacattg gagactgtgc
     3601 atattggcag cgaccataat ttaattgatt gtcgtttccc tgttcaatat gttgttcgtc
     3661 cgatgagcaa tgaatatcac gattatcgcg gttatgccgg tcgtgttgca gggggtgtat
     3721 tgaagcccgg cgatgaagtg atgcatttac ccagtggcat tacaacaaaa attgcagaaa
     3781 ttaaatccct tgatggcgat ttagaggaag catatcctcc gcagtcggtt acattgattc
     3841 ttgaagatga tgtagatatc agccgcggtg atatgttagt gcgcgaaaat aatgcaccta
     3901 agaccggaca agatatcgat atcatgttat gttggatgga cgataaaaaa tcgctgcaat
     3961 tgaatggtaa atatgcaatc aagcatacta cacgcgatgc acgatgtgta gtgaaagaaa
     4021 ttttatataa agtagatgtg aatacgttac atcgcattca ggatgtagat tcattggcat
     4081 tgaatgagat tgggcgtgta aagattcgca caacagttcc attattttat gacgagtatc
     4141 gtcgcaacag gaataccggt agtatcatca ttattgatga agcgaccaat acaacagtag
     4201 gcgccggtat gatcattgag tgatattgac caaacctaaa accaaagccc tgatggagag
     4261 tattcatcgg ggcttttttt gtggttgtgc ataaacggtt tgattcgagt agaagcggtt
     4321 actcgtttta aacctttctc gtctaagacc tttcgtgttt aagacctaac agttcttaga
     4381 cctgttaggt cttaaacacg aaaggtttgt cagggagaat tttgaccgga atttcaaccc
     4441 acaaaaaaaa gcccgccaac catgacgagc tttttctata tcataaaatc aatctattct
     4501 ttagtgaaat aagtaatgta attaccacca tcttgatgca ctaaaaccat ataatttccc
     4561 gcaggcaact ctgcgatatt tagggttaga atattttcaa tgaattgcaa ttccttcaca
     4621 ggcagttttt gccccaacat attaatgatc gaaacatcgt gatcacccga tacattggga
     4681 atattgacat tcaatattga tgacgccgga tttgggtaag caatcacatg gtttatgtcc
     4741 aacgattgaa tatcgtcgcc tgcaaaaatt acttcacaat acgtatcctc accaattcca
     4801 tttgacgcag ttaagcaaac attatattct ccgatattgg catacgtgtg tgatggattt
     4861 tgtaatccac ttatacttcc atcgccaaaa tcccacgacc aatcggttgg tgcatttgtg
     4921 ctcaggtcgg taaacttaat ggttaatggt ccaacattga tgtatgaaaa tgctgctgtt
     4981 ggcgcgcctg ccgtacttga aatagttaca tcctgacacc cggtatttac tcctccggga
     5041 cctgttacag tgagacaaac gctatatatt ccatccgtgg cgtaggtatg cacagggttt
     5101 tcgactgtag aaaatgcacc atcatcaaaa tcccagaacc aggaggtggg agttccggtt
     5161 gagagatcag taaatgaaac aaccggatcg ccactgtatg agaataatgc cacaggtgca
     5221 gcatatccat taattaccac aaccttacaa tcggttccgg agccaattcc gtttgtagct
     5281 gttaaacaaa cattatagct tccattggta ggaaatgtgt gaacaggatt ttcaacggta
     5341 ctaaaggtgc catcatcaaa atcccaatcc cacgatgttg gactattggt agaaagatct
     5401 gtaaatgaca cagttggatc accggtgaaa ctgaagtcga caaccggaga taaatagctg
     5461 tcaattacca catcttgaca tgaagtgctc gtgcccgcgc cattagatac agttaagcat
     5521 acatggtagg tgccgtttac tgcatatgta tgtaccggac tggcaatggc agaggtaaat
     5581 ccatcgtcga aatcccaact ccaggatgtt ggtgagccga tggaaagatc tgtaaatgta
     5641 acagtaggat cgccgctata aatccagtaa gctgttggtg caacgtatga atcgatagta
     5701 acattttcgc aatgtgtatc tgaccctgca ccgtttgtta cagtcagaca aacattataa
     5761 acgccattat cactaaatgt atgaacagga tcggattcag tgctcgtaaa tccatcgcca
     5821 aaatcccaat accattctgc cggtgagccg gtagaaagat ctataaatgc aacttcagga
     5881 tcgccgatgt aagtaaaatc agctacaggc tcattcatgg cgattatttc ttcacacact
     5941 gtagtttcac cataaacatt atatcctgtc agacatacat tgtatatacc cggagcataa
     6001 atatgcgttg gattttcaag agtagaggtg ccaccatcgc caaattccca ataatattct
     6061 tctgcatcaa cagagagatt tgtaaatgtt gcagaatatg tcattccaac aggaatcaag
     6121 gtatatgtaa aatcgactgt tggattatat ggatcgaatc gcgaaggatc gaatgcatta
     6181 tataaaatat tattgattac aatatcatca gcaggagaat cctgagtggt tccgggttct
     6241 tcatcctgtt gccactgcag gtgaattttt ccatccatta cttttggaaa tgcacttgca
     6301 tatgcatttt catgtcccag ataagcagtg tatgtaatgt ttacaggtga tgtccacgtg
     6361 gtgccaccat catcagaata gcatccgaat atatcatagt acgtttgatt gccggccgat
     6421 atttcatatt caaccggcat cataaacatt aaatacactc tgccggaaga tgggtcgtaa
     6481 catgcagttg gcattgtagt gacactttct gcataatacg cagtgaaatc acttcccaaa
     6541 cctgcatatg gatcatccag gccgtcgtca ttattccaat caaccatcac atccaacact
     6601 gtagcggagg ccatacctgt atgccaataa tatatgccac ttgttttggg aatgtaataa
     6661 tatccggcag cagtggtagg atcataaatg cgcattcttc cactaaatac atgcacagtg
     6721 ccatcgtctg ttattatcat ttcatgcatg ccatcagttg tgtaaatggt gtctttatct
     6781 ccatcgccat cgtaatcaga agtttgacct gcagcaccgg tataattatc gataggaaaa
     6841 tttactaagg tttgtgatgt ccatgttccc ggatcacctt ttgatggaga atgcattaaa
     6901 atcaaatcac aaaaattagc tccgtaaagt acgtagacat cattgccatg aactactatt
     6961 tgatagcaat cgggatttac taattcgatg ccgtatgctt cttcagtaaa tggaagaaaa
     7021 tattcattta ctgcccacgt ttcgcctcca tcatcggaac gggaaaatag taacgattcg
     7081 taggtgatct tttttgtatt aacaacgtaa atgtcatcgg taccttcagg gcaaaatgcc
     7141 atcggattat atccgtctat tacatttgaa ccggcagtct ctgtccagtc ggtggctcct
     7201 actgtgctgt ttttatttac gagcaaacgc gtagtttcat cagaaataac gacctcgtgg
     7261 cccatcacct ttaatagctc gccaaaatat gttttttgat cctccagtct tccgcttgga
     7321 aatgcacccc aagaggttcc gttgaagtga ttataaaaca ttccgcgatc attgaacgct
     7381 gcgccaaatc cgtcggatcc aacccaggcg gtgctgacct ttccatccgg atataccaac
     7441 agccggttgt gatttgccca ataagactga aaatcgaatg ttgtgttgcc aaccaaggtg
     7501 gtttgcgccg aaaggccggc tgttacaatc atgaggagaa tgctgatagt aaaagctttc
     7561 atcatagtat actattgagt gtaagaaact gtatataaac ttacaccata ggatacagag
     7621 cgtgtttatt taagtattat gctttcaggt tacggcggtc acattttgat aaatatttgc
     7681 aaaaaaaagg cggaacaatg tccgccttct tcaagatttt aaattattta aaattttata
     7741 atattactac gatataaata gttttcgatg ataattttaa cagtatgtac acctgaaggt
     7801 agcgaagaaa cgttaacctt acaattatta atatgccatt ctaccttgtc actaaagttg
     7861 atctcttgtc aatttactga gcttacatat actgctgcat tggtatgttg ctccggaagt
     7921 tcctaattaa taataaggat ttacccattg aattttgcaa aataaaaaag gcgagacatt
     7981 gtctcgcctt tcaatgatat tgttggaatt acaatttaac aaactgtcct gtaaacagct
     8041 gattatcaac cagcatcttc accgtgtagg tgccggcggc tagtttgctt acattgataa
     8101 tgcaaatgcc attatcccag ctgattatac tgttgtcgcc aacttcctgt ccagttacag
     8161 acgtaatatg aatatcgcct gaaagtgtgc ctgcaggcag tccttctatc atagtatatg
     8221 ttccggcagg atttggataa caattcaatg tgatttgatc cagaacctgt atagcattat
     8281 ccgttacatt tattgattta caatcagtgt tggttccaaa atcatttgtt gcggtcaagc
     8341 aaacttcata tacagcttca actgcataag catgtgttgg gttttgaagt cctgagatcg
     8401 ctccgtcacc aaaatcccac aaccaatctg atggcgaatc gcttgaaaga tcagtgaaag
     8461 ctacagttgg tgaattgatt acatatgaga aatcaattgt aggagcagat ccattacctg
     8521 cgatggttac atcagtgcaa tatgcatcag aacctcccgg actcacaatc gacaggcaaa
     8581 cattgaatat tccatttgtt gcgtaaacgt gtgatggatt ttcaagtgta cttacgccgc
     8641 cgtcgccgaa attccataac catgaatttg gactgccggt tgagagatcg gtaaatgtag
     8701 ttaccgggtc accgctgaat gagaacaatg cgcttggtgc aacatagcct gtaacaataa
     8761 ttgttttaca ttctgtatct gaacctgcaa tattgccggc tgtaagacat acgtcatagg
     8821 tgccatcaga agcatataag tgaataggat tttctaagga agaagttccg ccatcaccaa
     8881 aatcccatgc ccatgttgta ggtgcatttg aactgatatc agtaaattca acatcaggat
     8941 caccggtgta agtaaagtcg gcaaccggaa tatagacata tgaatctata attacatcct
     9001 gacaagaggt gttacttcct acggcatttg ttgcggtcaa acatgtatta taagttccat
     9061 tggttgtgaa ggtgtgtgtt ggattttcca gtgtacttac accaccatct ccaaacgacc
     9121 atgcccagct tgtcggactg tttgttgaaa gatccgtgta agttacggtt gggtcacctg
     9181 taaatgtaaa tgcagccacc ggtgataaat atgaatcgat ggtaatcaat tgacatgatg
     9241 tgttagatcc ggttgcattt gttgcagtta agcaaacatt atatacgcca tttgatgcat
     9301 ataaatgaga tggattttct aaagtagaag tgccgccatc accaaaattc cagaaccagc
     9361 ttgtagggct attcaatgat gcatctgtaa agctaactgc aggatcgccg ctgtaggtaa
     9421 atgcagcaac aggcgcgaga taataatcaa taaccacaaa ttggcatgaa gtatttgaac
     9481 cggttgcatt tgttgctgtc atacatacat taaatgttcc attataggtg taagtatgtg
     9541 ttgggttttc taaagtagaa gtgccaccat caccaaaatt ccagaaccag ctagttgggc
     9601 tgttcaatga tgcatcaaca aatgacaccg ttggttcacc actgaatgta aaattggcaa
     9661 caggaggtac ataagaatca attaccacat tttgacaggc tgtatttgat ccatataaat
     9721 ttgttgcagt aagacaaaca ttataggtgc cgtttgttgc gtaaaggtgt gccggatttg
     9781 ttgctgttga aataccgcca tctccaaacg cccagctata tgaggtaggt aagccggtag
     9841 aggtgttagt aaaagtaaca gttggatcac ctgcatatgt aaaggctgca gttggtatag
     9901 tgccgggaat aatatttaca ttataatctt ctgtttctcc aaaaccatat tcatcacaag
     9961 gcaaaatccc tatctgattc caaacttctc ttacgcgcaa tcgggttgtt ccggttgcag
    10021 ctgtaacagg cactgtaaaa atgatattac ctgtagccat agccgcaagg ttagtcactt
    10081 cacctaattt ttcagaagct tcaaatacac cattatgatt ataatctatc catgcagcga
    10141 aaccattttg tgtggcatat gtgcctaccg ttacacttaa tgtatttgat gtgccggcca
    10201 ttaaatcagt tgacaatgct gtgtaataat tatagaaagg agcaggtagc gctccggttg
    10261 tgttatttag tgttcccaat actgcgtgac taatataatc gccttctgta gtaccggtag
    10321 tatatgttgg tatacaataa caacttgttg ggtcattttg gtttacagta acagggcttg
    10381 aaacttcaaa atctccgctg atggtacaag ttactttaca acgatataat gttgtggcaa
    10441 tttgcgtagt tgttaaacta gacgacacag ctcccggaat taatgaatat gccgcaccgt
    10501 cgggagaaga gtaccaagta taggtaattc ccaattctgc aactggtggc actgataaag
    10561 taaattcttc accaatacac acattggttg cggtagtgtt tgttacacct aagcttggaa
    10621 caacggcgca atcagtaccg ggaatagttg taaaaattac ttgaggtcta tagtccggac
    10681 cgccgggaga attctcccaa tattcggtac ctgtataacc acatgggtca ggctctgtat
    10741 cgctttgtcg tgtatgagat ccattaaaag ctaatgggcc tgtccatgta acccttgcat
    10801 tttttgtgaa tgttgcatta aagtcgccac cacaaacttc tactataata cttgaaacac
    10861 catcccaaaa gaatggagat gcaagcgtaa atgtgttgat tcctaaaaca ggattataaa
    10921 atgttggtcc ccatactgta gttgcaccct cttcccatgt tgtaagtcca agtgaggtag
    10981 tgccgctatt aaggagtttg atagtgtagt cttcagtacc atcaatagtt ccaggaagcg
    11041 cagcaacgtt ccaggctatt tcggtgataa atccggccga catacccatg tcagtaagct
    11101 cagatgcacg atatagatat tgtgcacgca tggttttgta ataatcaccg aaaggtgaag
    11161 ggtagtttgt aggacccggc ataccgttgt cggctccaat tgtaaaggtc tgtgccgtta
    11221 agcaggaact gattccaagt attaagctga gagtcagtag ttttaatgtg cgttttttca
    11281 taactggctg gttattaatg ttgtcgtgta atttgtagtg ttttggtaaa aagttaccaa
    11341 aagtaggggt tttgcactaa aaattggtaa atatcaatgc tattatacct gctatatact
    11401 gtcaggtttc ttcaaatcct tacaaaataa aaggtcgccc cataggaggc gaccttttaa
    11461 agtgctgaat atcaataagt tattctttca caaactgtcc aatgtaagcg gcattgccat
    11521 ttgtcatata cagtgtatat gcgccggaag ctaattcccc tatattgatt gtaagtgttt
    11581 gattctgaat agcttggatg ttagagttaa ccactttttg acctacggta ttaaaaatgg
    11641 ccacctgaac atcaccggtg attgccggaa gtgcaatgga gatagcacct tcagcaggat
    11701 taggccaaac attcagatta gttacattca atccggcaat agctgttaca tctacaacct
    11761 tacaaacatc gtcgccgccg cctgcgtttg aagcttcgag gcataccgta aatgaaccgc
    11821 cggctgtgta aacgtgaacc ggattctgtt cagtgcttgt accgccatca ccaaaatccc
    11881 aagcccatgt ggtaggtgaa ttacttgagt tatcaaagaa ggatacggat cctcccacac
    11941 caaagaagaa gttaaagtcg gcaaccggag cggttacaac ggatcctatc acaacatctt
    12001 cgcaatacac atcaacacct ccggggcctg tagattcaag acaaacatta tatgttccat
    12061 tagaagtata agtatgcacc ggattttctt cgctgctaaa tgagccatcg tcaaaattcc
    12121 agaaccatga agtggaattc acagaaagat ttgtgaagat aacgtcggga tctcctgagt
    12181 aagtaaacat tgcatcagga gccacatatg atgagattac aactgcttcg caaccggtag
    12241 tagaacctac tgcatttgag gctgtcagac atacattgta ggtaccattt gcagcataca
    12301 tgtaaacagg gttctgttct gtgctgaaag acccgtcgcc aaaatcccat gaccatgaag
    12361 ttgggtcttc agttgatagg tcggtgaaac taacagttgg atcaccggtg tatgagaaca
    12421 taactgtagg tggcaaatac gaatcaataa ttacatcctg acagcttgtg cttgagccaa
    12481 gtgcattagt tgcagtaagg caaacattat aagtgccgtt agtagcatat gtatgtaccg
    12541 gattttgatc ggtagaaaca ccaccatcat caaaagtcca tgaccatgat gttggatcgc
    12601 cgccggttaa atctgtgaag ctaactgttg ggtctccggt aaatgagaaa ttggccaatg
    12661 gagctgcata gctgctgata attacgtttt cacatgctgt atttgttcca ccagcattag
    12721 taacggtgag acaaacatta taaatgccat taaatgcata tgtgtgcacc ggattttctt
    12781 cagtagaagt tgcgccatca tcaaaatccc aataccaact tgtaggttca tcggttgaaa
    12841 gatctgtgaa tgtaacagtt ggatcacctg aataatcaaa taatgctacc ggtggaacca
    12901 cagcaataat ttcctgacat actgtagctt caccataaac attataacct gttaagcaca
    12961 catcataaac gccttcggca aatgtatgcg tagggtttgt tagtgttgaa gtgccgccat
    13021 caccaaaatc ccagaaatat gtttcggcat ctgtagatag attggtgaat gttgcattaa
    13081 atgtagcgcc gactgcagtg agcacataag aataatctac tgttggagca tatggatcga
    13141 agcgtgaagg atcccaatcg ctatataaaa tataatttgt gtgaattgga tctgcaggaa
    13201 catctacagc cgtacccgga ttatcatcct gttgccagat catatgaatt ttaccatcaa
    13261 caacgcgatc gtataccata ggaaaataat tttcttttcc cttaaacgcg ctgtacgtaa
    13321 ggtttacagg gtatgaccat gattcgccgc cgtcctcaga ataggtgccg aaaatgtcgc
    13381 atttagactc agaagttggt aatgtcggat caccgaattg atcggtgtac tcaaccggca
    13441 ttgtataggt gagatacaat cgtcctgtag caggatcata agctgctgat ggcatagaag
    13501 taaaactttc gccaccataa gcaataaatt gttgtccaat tcctgcaaat ggatcatcca
    13561 gtccatcatc atttttccag tcaaccaaaa cgtctaaata atacatgcca accaatcctg
    13621 ttttccaata aaggaatccg ctgatggtag gaaaatatga ccatcctgct gttgttggat
    13681 catcgtcaag catatagtat gcaggagcga atacttcgag tgtgccatca tcggtgataa
    13741 gcatttcgat attgccatct gttgttaaaa tggtatcata atcgccatca gtatcatagt
    13801 cggtatcctc accaacaaga ccggtataat tttcaagtgg gttatcccaa atgatttcag
    13861 aagtccatga tcccggatcg ccatttgcat cagaatgcaa cagcactaag tctgtatatg
    13921 acgcaccgta aagaacataa acatcagaac cataaactgc aatttgataa gcttcggctg
    13981 taatagctcc gaatccttct gcagtagtta gatatggtaa tacatattcc agtacttccc
    14041 agttttcacc accatcggta gaacgtgaaa aataaatctg agtaggtgag ctggcgtttg
    14101 catttactac gtaaacatcg ttggtgccgg cagggcaata agcatttggc caaagacctg
    14161 taatatcggt agaacctgct gtttcagtcc agcttgttcc gccgatggtt gcgtttttat
    14221 acacgcggat attggttccg tcatgggaaa gaacgatctc atgatcgccc acattaatga
    14281 tttcgccaaa tcctgttctt acgccttcaa ttcgagcagt tggaaaacca ccccatgtag
    14341 tgccgttgta tttattaaag aacatgcccc tgtcggagaa agtggctgcc aggcttgttg
    14401 agcctgtcca tagcgctgaa accttgccat cgtcatatac atggaggcgg cttttattgg
    14461 cgctgtttgt ttgtagatcg taggtggtgg ttccaatagt cacgggctgt gccaacactg
    14521 caccccatgc taccacaccc aacatcaatg agtaaatttt tttcatagta tgatgtaaat
    14581 taagtggacg caatttaggg aaaattgttg aattagccga tgatggttac atttgggata
    14641 atatctttag aatgaaaaac accctcagat tcccgatcgc agctctcgct tttcttgttt
    14701 taccactaat agccaccgca caaaatggaa caccggaagc agggcgacaa gactccttac
    14761 ctgtaatgga taccaccgat catacaccta atatgatcta tgaatattta gaaacaatgc
    14821 cggaattccc gggaggcaca atggcactct atgaatttat taatacaaat cttcaatatc
    14881 ctgaggctgc caaaaaaaag aaaattgaag gtaatgtcta cgcaaaattt gtagttacag
    14941 aggatggttc tataattaat attgctatat tgcgcgacat aggagggggt tgtggcgagg
    15001 aagtggtacg catattgtca actatgccaa aatggaaacc tggcatgcaa aatggtaagc
    15061 cggcagcagt attttacaaa cttcctataa aatttacatt aagcgattaa cctgaggcgg
    15121 agtaattatt ttctcaatcg ccattcggtt ggatagtaat tattatgtct tcccggaatc
    15181 atcttcaccc aacctaatgc aatattttca taggtaataa tatgccatcc gggttctgat
    15241 gagtttaatt caatattatt tttccttaag tattctaatg cctctgattt tgatagggcg
    15301 atgcgtggaa tattgtctga aatgatattg ctcatcgcca atgcatgatg tgggatcaga
    15361 ctaccttgct taatttctcc gattgctata cctgcctgca gaatatttag tcctgatgcg
    15421 gcttttaagg cttcttgcag ttgtttcgtc caggcaaaaa atacattgtt gtgcttaacg
    15481 aaaatgaatt tatcgaaatt cttcagccat gggcgaatga tttcacgctc ttcactagaa
    15541 gctagctgat aatttagttg tttgcctttg gaaattgcta attcttcagc gttgtttttt
    15601 tgtaagatgc tgataaaaaa tccttcacca cgaattttgt ccggataaaa tctatatcct
    15661 aatgctgttg aaataatacc ttgtgtttca attgatgaaa taatatcatt tctgtttgtc
    15721 aaaccttgtt caataaaatg cgaaactaca tgttcgtctt cctccatact atatgagcat
    15781 gttgcgtaaa tgagaaatcc tccgggtttt agtgcaggta aaatatctgt gagaattcgt
    15841 tgttgacgtt tgctgcaagt aattacattt tcttcgctcc attctgctat tgctgcaggt
    15901 tccttcctga ataatccgga tccgctgcat ggtgcatcaa caacaatgat atcgaagtat
    15961 tcctgcatgc ccgtgaaagt cgccggatcc tgacttaaaa caaccacatt ttcctttccc
    16021 cactttgtca gattttctct taaaatcggc gcgcgggttt taattacctc attgctgaat
    16081 aaaatacttt cttcatgcat agctccggct atcaaagtgc tttttcctcc cggtgcagca
    16141 cataaatcta atatgcgcaa gggcttttta tctgcatcaa gttttctaag tatgaaatct
    16201 aaaaacatgc tggaagcttc ctgcacataa tacgctcctg catgaaacaa cggatcgtaa
    16261 acaaataagg gtctttccgc caggtaacat gcgttttcgc accatggcac ggggtcatcg
    16321 ctcaggcgtt ttgccgattt caatggatta taccttatcg atacaggggc cacctcgtca
    16381 tgtgacgcca caaaagcggc tttatcgaaa ccgatattgt gttgtaagtt tgctataaat
    16441 cccggtggga atgacatatc taggtccgac aaaggtaacc gctattgcat gtaattaatc
    16501 ggtttttaag ctaaactctc ccggaaagta gatttgggtt gagcctaatt gcttaacttt
    16561 aattttaagt ttagtttatt catgttcaca cgcaccatac tcctcattgg gcttctaggc
    16621 tcattttgct tcgcgaaggc gcaacttgtc atcaatgaaa ttgcaacaaa cggagcagtc
    16681 ttagatgctt ataacgaaga aagcgattgg ctggaattgt acaataattc agctactgcc
    16741 atcgacctta acggatatgc actgaccgat gatccgctct ttccgaataa atggcctttg
    16801 cccgccatca tgcttgatgc taatgagcat ttattgttac tggcaaatgg aaataatact
    16861 tatgcgctta tcgaccattg ggaatcggca gtattaaatg aaactgagcg aacatatttt
    16921 gttggcacaa gtgaaccgcc ttccgattgg aatactgtcg gatttgacga tacaggctgg
    16981 agcaccggac ccggaggcat tggttttggt gatggagatg ataatactgt aatagcttct
    17041 gccacctcgt gctatatgcg ttgtgcattt gatgtggcag atgtaagtgt aattgaaaat
    17101 gcaaaattgc atatggatta cgacgatgca tttgttgctt atttgaacgg cgtggagatt
    17161 gcaaggtcgg gtaacattat tggaacacct ccgacttaca atactttagc aaatgccgat
    17221 catgaagcgg ggatgtatgc cggatatccg gctgaagaat ggaatattcc agcagtggtt
    17281 atgaatatat tattggtgcc cggtgaaaat attttaagca tccaggtaca taacgttact
    17341 gcaggctcat ctgacctttc atcaaatgcg tggttttctg ttggcatcaa taacggactc
    17401 acatattttt cacccgtgcc cgcctggttt gcagaaccgg tagtatacag tgaaacgaat
    17461 tttaaaatca ccaataccgg cgaaacaatt tatttattcg acaataccgg tgcaattgca
    17521 gatatggtaa atccgggaag cgtagcttat ggtcatgcct atgcgcgcat tcccgatggt
    17581 gctgcaagtt ggtgcacaac tacaacaccc acacccgata attcaaatga tggcgcacct
    17641 tgttttgccg gatacgaacc gctgccggtt ttttcgctcg ctgccggatt ttatactgga
    17701 acacaaacat taacgctttc gtcaacttca ccgacagctc aaattcgata tactactgat
    17761 ggttcgcttg ttacggcaac atcgccggta tacaccggtc caataacaat ttcttcaaat
    17821 gaagtggttg ctgcgcgatg ttatagtacc ggaacattat taaccggaga gcaagttaaa
    17881 cgctcctatt ttatcgatga agatgactac accttgccaa tattatccat ctcgatagac
    17941 cccggaagtt tatttgacta tgataccggt atttatgaat tcggatgttg ttacgatgca
    18001 gcatatccat atttcggggc taatttctgg caaccatggg agcgcttcgg acatatagaa
    18061 tattttactg ctgatggaga ttttgaatgg ggaaaagata tgtcgctgga aattcacggc
    18121 ggctggagta gggcagaggc tcagcgcggt tttagggttg attttaaaaa taagtacgga
    18181 ggaaatctgg agtatccatt attcggcggt aagcctgatg caagctccat aaataatttt
    18241 aatatacgca gtggaggcca gcacgtgtgg acatataaaa ttcaggatgc gtttctggca
    18301 aaggtgatgg aggatacaca tattgattat gaagagtggc aaccatgtat gttgtttata
    18361 aatggcgatg catggggatt atacgagatc cgcgaaaaag ctgatgaaca ttttgttgaa
    18421 tcgaattatg gtatcaataa taatgatgtc gatttgttga actcatattc tgtgttgaat
    18481 ggaagcgaca ccggttggtg gaatatttat tccagtctca tgtcgttaga tccattgtct
    18541 gatattttct acgataagtt caatcaggca tttgacatgc agaattatat cgattactac
    18601 ataggtgaaa tttattatca gaatgtagat tttggtggat attattgggg ggcgaataat
    18661 atgaaggtgt ggcgaaacag gtatggcggg aaatggcgat ttatcatgta tgatatggat
    18721 ggtgctatgg gatactttgg ctcttcacct tatgataatt atatcaacct tacccgcagt
    18781 ccggcatatc catcttcata ttctcagata tttgatcgtg tgctcgataa tcaggagatc
    18841 cgtgaatatt ttgtgaatcg atttgccgat ttgatcaaca cgatttatca acctgataat
    18901 atggaagcag tattgtacga aatgcgcgat tctattcttt ctgaaatacc acatcaggtt
    18961 gagcgatggg gcgctccaaa tacggcaaca gtaaatagct atgtagatgg cctgttgagc
    19021 tacaacaatt cccgcaaaaa tgcagcaaga acacatatca ataatagttt cgacctcgat
    19081 ggtcagcgca caactacttt agccgtgagt cctgccggtg ccggatatat taaaataaat
    19141 accatcgtgc caacagaatt gccatggacc ggcatttatt tcgatggtgt gccaattaca
    19201 attactgctg taccaaatcc cggctacaca tttgattcgt ggttgccgaa tgccttgctt
    19261 ccgggtggtt ccactgatat atcgttgacc ataaatatgg ataacagcac aacatttacc
    19321 gcagtattta caggaacggc tgtaactccc gaaattattg tatctgaaat aaattataat
    19381 agctatgttg attataatat gggcgactgg atagaattgc ataatgcggg aactgatgca
    19441 attgatattt cttactggaa aatcaccgat cgatatgagg gtaatgccta tatcttgcct
    19501 gcgggttctg taatcgagtc ggatgagtat cttgtaattg caaaagatcc gatagccttc
    19561 tcaaacgtac atcctgatat aacgaatgtt gttggcggct acgattttga atgggataat
    19621 tcaggtgacg atattagatt atttgacatc gccggaaatg aaatcataca catggcatat
    19681 gccgattcac ttggctggcc acaaggtgct gatggaaccg gtcgcacatt ggaattaata
    19741 aacccgactg cggatatgaa taatcccggt agttggttcg acggatgcat cgccggttca
    19801 cccggagaag cttattcgcc atgcaatgat gcactcgttt ttagtgaaat aaattacaac
    19861 agctccgcag caatgaatgc aggcgattgg gtggagatac tcaatactac agctgatgat
    19921 atggatattt caggatggaa attccttgat ggcgaagatt ctcttattta tacatttgaa
    19981 tcaggcacta caattccggc aaacgaaaga ttggtattga gcggcgacct cgatcttttc
    20041 gaatcacgcc atcctgatat tacaaatgtt accggcgcat ttaattttgg attagatgct
    20101 tcaggtgaag agatcaggtt gtttaatgat catggtattt tgcagtttac cgttatttac
    20161 gatgatgctg cgccatggcc atatgaagcc gatggtgcag gaaaaacact tgaacttgaa
    20221 agtgcttcag gcatcatgaa caatggcgat aattggttcg ccggttgccc tgaaggttcg
    20281 ccgggaacta tttatgaccc tgattgcgac ggtgttattg ccatcgaaga gtctgttccc
    20341 gcatccttgc ttgtgttccc caatccggct aaaaattatg taatgattgc cttcgaaaat
    20401 aattacaatt cgggtagaat tgaattgtgt aatttatccg gacaaattat ctatgaaacg
    20461 caatctctcg aaaacggaca agtaattata ccgcgcaatg aaatcgcgtc aggagtttac
    20521 agagtaagat taattgccga caaaaaaatg atagagagcc tgttggtatg ggagtaaacc
    20581 aacaatttat ttttttcgct cagtagtaaa tattacgaga tcctttaaag cgcgtttgct
    20641 ctcgctttca ggaaattttt caagtagttc gaaggcttct tgttggtatt gtttcatttt
    20701 ttctgttgca taagcaatgc ctccgccctt ctttacataa tcaatcaccc aattcacttt
    20761 ttccttgtcg gtattgtagt gtttgatgat gcgcataatc tcacttttgt cattttttgg
    20821 agcatgtaaa agtgtgtaga taataggaag tgttaatttt ctttctttta tatcgatacc
    20881 aagaggtttg ccgatatcat cggtgccgta atcaagtaaa tcgtctttaa tttgaaatgc
    20941 aatgccaatt ttttctccga aaagacgcat aagcgaaata gtatcttcat cagtggttac
    21001 actggcggca cctgcactgc atgcactggc aatcagtgta gcggttttct ttctgataat
    21061 ctcaaaatag acatcttctt tgatatcgag gcgacgtgct ttttcaattt gcagtaattc
    21121 tccttcactc atttctttta cggcatcgga agtaatcttt agcattcgga attcattatt
    21181 gtcgagcgac actaataatc cttttgccaa taaataatct ccgatcaaca cggcaatttt
    21241 gtttttccaa agcgcattga tgctgaaaaa tcctcttcgc tcataggcat catctaccac
    21301 gtcatcgtgt accagtgttg ctgtatgaag gagctcaatt aatgtggcgg ctgtatagga
    21361 agattcagaa atattgccga tcattttcgc actcagcaat acaaacatgg ggcgcatttg
    21421 cttgcctttc cgctttacga tgtaatacat caccctatcg agcaaggcca cattgctgcg
    21481 catggaggcc ctgaatttct cttcaaaaag ggctaactcc tcgtgaattg gctgctgaat
    21541 ggaggcgata tcggccatgg ggctgcaagt taaggcacat tttttagatg taacaaaata
    21601 cctctgtttc cgtttaacat gtgaaagggg cgcttgcatc atgttgagag cctctcgttt
    21661 tttatgttta aagtgaatag aactgttgtt attgtaagct gcttcggtat gttcctgagc
    21721 ggcataattg ccatgcaatc ctgttctgaa gggggtggga tcaatatttt ttctattgaa
    21781 gacgacaaag ccttgggaga ccagatagcg gcagaaatag caggcgatcc tgtaaattat
    21841 cctatccttg atagcgtcga ctatgcagat gcttatgcct tcgtttatga tatccgtgat
    21901 agaatccttg agagtggtat ggtgtttcac aaggatgatt ttaagtggca ggtgcgtatt
    21961 atagatcagg attccgtgat caatgcattt tgtaccccgg gaggttacat ctatgtgtat
    22021 acggggctca tcagatacct tgagaacgaa gctcagctgg caggagtgct tggtcacgaa
    22081 atggcacatg ccgacctgag gcattctacc gaccagctta ctcaggctta tggtatatca
    22141 atactgttgg atattgtcct gggtgatgat caaaacgcaa ttactgatat tgctgcaagc
    22201 ctgactcaac ttgcattcag cagaggcgat gaatcgcaag ccgatgaata ttctgtcatc
    22261 tatttatgtc ccaccgaata tgaggcctcc ggtgcagcag gtttttttga gaaaattgaa
    22321 gctgaaggtg atattcaaat tccggaattt ctgagtaccc acccgaaccc cgataatcgt
    22381 attgatgcta tttatgcgaa gtcgtcagaa ttgtcctgct caggtgatca aacttatgat
    22441 gcgcgctatc aggatttaat tgatgccttg ccataatcat ttgaacaata ttcataaaaa
    22501 aaccggcaaa gacttttaac atcaatgccg gttgttgatt tgttgctgtt tttttatagc
    22561 ttcactacta ccgtgcctga gaaggttctt ccatctgaag gccatatacc gggacccgga
    22621 tagaattgcg gacgtttagt aaaatactgt tcatcaagaa tgttgtttgc attgaattgc
    22681 acttttacct gattagtcag tttaacgcca atatttatgt cgatgagttg atacgaagga
    22741 accaggccaa cagagccact ggcatttggt tctaccgtat ttaatgcgtc ggcataactt
    22801 tcgccaacat aactatataa agctgaaagg ctgtatcgag tatatttcaa tgtcagtccg
    22861 ttgcgggaaa taatatcagg aacactctct actttattgc catcaatgtc gatattttca
    22921 ctgcctgaac gaatggttgc atcatggtag cgagcatcca tatatgaagt tgatgtaaat
    22981 attgataaag acaattttct tgtgagatca aaatcgccct gcataaagaa ttcaagaccg
    23041 gttgttcggg agtcgccaat atttgtccta cggataatca gattcccggc tgtatctgtt
    23101 tctgccaatg ttcccaagcg attgttatat gctaagtaga aagcggttac atcccatttt
    23161 aaaaatttcc agtttcctct atatccaacc tcagcgttgt aaccgtatgc atcttttaaa
    23221 tctttatctg catattcata tacagaagca ggtacgatgt ctttaaaaat caccggtcta
    23281 tatgcctgtg accatcctcc ataaatattg gtgaatttag atacattgta ttgtgcactt
    23341 acaccaaaca atgggaagct gtgtttaatg gtgtttggca aagttgaatc gggataatat
    23401 gtgattacac cactcatatt gctctcaccc atttctattc gcgcgccggt gttaaccgaa
    23461 aacctatcag agagcaccca cctgttttct gcaaatacag caatgttgct tgttatgaag
    23521 tgtaaatctc gtccccagcc cggtatagcc aatgtaagat cgaagtcgct accggtagtg
    23581 cctttgccaa tttgctgacg atgtaaatca ttatacatgt attgcacacc tgctgcgaga
    23641 ttgctggtat gacctaataa tttataggat tgtaacaaac gcaattcatt tgtgtagctg
    23701 ttgaaatgat caatatcaac ctgacgatta ttgtaatcga gtgttgctcc aacaatggta
    23761 tcttctgtgg tggcaggtct gtcaaacatc acgctgtttc tggcgccaat taccgctgat
    23821 gatgttagct gtaatttagt agatgatgtt atattccaat tcatgttaat tgaaggaaca
    23881 tgaatattag ggttataata atttcttgaa cgtgtagcca tttgcgggtc ggcataaaac
    23941 atgctgtcgg ttaatggacc cggaagatgt gtaatataat tggagtgcgt ccactcaaat
    24001 ttgaaggtca gatcttttga atagtcgtaa aataatgaaa cagcttcagc atcatattgt
    24061 gcgtcgctgt tatctcgata tcctgtaatc cattttttat tataccaggt agaatatctg
    24121 aattttccaa ttttacccga tgcgccatta taggtgctta ctaaaccgta ggagcctacg
    24181 gtattgcttg tttcaaaagc caatgcacgt gtagaatcag gttgtttgct gatgtagttt
    24241 aacataccac caaattgtgc accatattgc aatgatcccg ttccacgcac taattcaatt
    24301 ttctcaactg cttccatcgg gatattatag tgacttgcag gataaccata catatcggag
    24361 tttgtgagaa caccatcctt tctgatattg aactcccaac cgcgatgcgg atctaagccc
    24421 ctggttgaaa tgtttatttg attgccggtg ccgtccatgt cataaacaaa tacaccaggt
    24481 atttttgaaa atatttgacg accatacttc tcgctcagcg acacagtcct ttgtgttaga
    24541 tcgataactt ccgtcttttt gcctgaataa ataaatgtcc ctttaacagg ttgtatacgc
    24601 tgaatatccg gaatggtgcg ttcagcttgt acatttaccg taggcaacaa cttcagtttg
    24661 ctggtgtcag tttcagccgt ttgggctgaa agggctgcag aaatgcatac aatagtaagg
    24721 aataggaaat atctggccat tggatgaatt tttgaatggc caaaactacg tatatgagca
    24781 gggctaacct ttgatacttt gtaaaagaaa agtaaacgaa atattaagag cgctcaagtg
    24841 aaggcttgtc ggcaatcttg ggagacgacg gacggccaac tcttgggttg agaattacta
    24901 attaaatttg tgcatgaagc gattgttttc attattgata cttgggtctg ttttgacaat
    24961 gaacggacaa actattcctt cgaaacctaa actggtggtt ggaatcgtag ttgatcagat
    25021 ggcctacgag tttttatatc gctatagcga tacatactca gattctggtt tcaagcgact
    25081 tttgcgagat ggctatagct gcgaaaacac ccattataac tacattccca cgtataccgg
    25141 tcccggtcat gccagcattt ataccggatc ggttcctgca ctcaatggcg tagtgagcaa
    25201 cgattggttc aatgaacaga gcggcgcata tgtttacgtt accgacgaca gcacagtatc
    25261 aatggttggt ggcaatggac tgggaagtaa aatgtcaccg aaaaatatgg tggccactac
    25321 cattactgat gcgttgcagt tgagcaatca gcaacgatcg aaagtgatag gaattgcatt
    25381 aaaagatcgc ggtgcgatat tgccggcagg gcatgcagct gatgccgctt attggtatga
    25441 tggcgccagt aatgcgtggg tgacaagtac ctattatatg caacaattgc ctacctgggt
    25501 gagtgcattt aatatgcagc aaccggcgca aaaaatgatg aataaaaatt gggatctctt
    25561 attgcctgca tctcaatata atcaaagtac agccgattct gtttcgtggg agacattgta
    25621 caccggagaa acatcgccga aattcccgca ttatttgcca tttaccggta atagcgaaag
    25681 tataaaaggt acacccttcg gaaatacatt gactgcgcaa tttgctatgg ctgctattaa
    25741 gaatgaagaa ctcgggacgg gtcctgaaac ggatttttta tgtgtaagtt tttcatctac
    25801 tgattatgta ggacatgcat acggcccgca ttctatggag attcaagaca cctacttgcg
    25861 gttggataaa gacttaggtg aattacttac ttatctagat acacaagttg gcaaaggaaa
    25921 ttatttatta tttctgactg cagatcatgg tgttacacca acaccacaat acatggaatc
    25981 attgcgcatt ccggctggaa caactacaga agtgtcgatg ttgcaaagta tgcaagcgat
    26041 gttgaatgcc gcatggggtc aaaaagactg gataagtgca tacaccaacc agcagattta
    26101 tttaaatgat gcattattgc aagctgcggg taaaactatt gaagatgcat ataatgttct
    26161 ctacccccaa cttatgaaaa tggacggcgt ttcttatgtc ttacaaatta ataaattgaa
    26221 tgacgccacc atgaacaatg atgttaaaga gcttgtcatc aatggcatct atcccaaacg
    26281 atgtggcgat attcaaattc tgtatgaacc ttattggttt gaagcctaca ggccaacagg
    26341 tacaacacat ggctcgcatt tttcttacga cacacatgtg ccgctgattt tttatggatg
    26401 gaagataaaa tccggtgcac tgcatcggaa tgtatatgtg actgatattg ctgcaacact
    26461 tgccgatctg ttaaacatca gtgagccaaa cgcatgtata ggtcatgtta tacctgaagt
    26521 ggcaaatggt aaataaattt tattgaagaa atactacgat gaataaaaaa attgcaatca
    26581 tcattattat cgggtcgata ttgttaacat tcgttagcat ggctattgtt ttcattttgc
    26641 aaaaagatga taataatttc catcacacag agacgaatcc cgcacctgtc agggtggatg
    26701 gtaatcatga gaaggaagat gaatcctata atcgcgacag acaaaatttc ttcgaccaaa
    26761 tgcataaatc tgcacccgga acaaattgga gaaaaatgga tgccgatttg cgatttcagt
    26821 taatgaaaca gcgcagtaat aatcttcgca ccgatgatat cgctatggat accctggcat
    26881 caggagcatt aattggcgaa tggaatgaga tgggttctta taatacctcc ggaagaataa
    26941 gagcaacaga agtagatttt gaaaatgaaa ctatatatgc attttccgac ggcggtaatc
    27001 tttggaaagg cgatctggat ggcgaaaact gggcggttat caatgataat atgaagataa
    27061 ccggagcaca tacgcttcga aaaattggtg atagattaat tgtaggttgc gaccaatggg
    27121 gtgttcaagg cgttttttat accgaagacg aagggcttac ctggaatgca acaaccggtc
    27181 ttgaaaatgt tgcagcatgg ggctatatct ctgatatgga aatattaaat gatgccaatc
    27241 acaccattta tttattggct tatgaatggg attatacaaa ttggtgggat atcgtttcgc
    27301 tatatcgctc agaagattta ggagtttcat tcactaagct cagctcatat gatgttcctg
    27361 tttatggtgg ggccaatcac ttcgttttgt ggtcaccgcc atacggctct cctgattgct
    27421 atatgataga aaatacgcac gttaatttac tcgatgccac cggaacacct gtattgcaag
    27481 gaacactgcc tataacagat gatggcgatt atatgttgtg tggttttgag ggaccttcct
    27541 ctacaagcct gtatgtggct cacagaaact atgtcgataa tttcacacat ttttatactt
    27601 ctttcgatgg cggtgtaaat tggaacacag agggtattat tgaggaaggg aatttcagca
    27661 agaatagttt taattgctct cagaaacagg agggatattt attttatggt ggtgttgata
    27721 gctataggag ttttactgcc ggtaattcat gggtgcggaa taattattgg tacgaatatt
    27781 atggcgatat agaatataat ctgcacgctg atataccatt tatcagaact tttattgatg
    27841 gtgggggaga tgaaatcgtt ttgatcagta ctgatggcgg cttatataaa tcaacagata
    27901 atggacttat ctggaataat atcactaacg aaggcatgcg caatgctcaa tattatgatg
    27961 tgtataccta tcgatgggta accgatattt tgtttgcagg ggcgcaggat cagggatatc
    28021 aacgcagtag ttatactatt ggtgatgact attattttga tcagttgatt agtggcgatt
    28081 atggtcatat tgtttcaaga agcggtggtg ataatttatg gtgcgtttat ccgggatttg
    28141 ccatgtatgt gaatgatgca gcctcggcat ccaatcttac tacatgggat tttcagggtt
    28201 atggtcacct ttggatggcg cctatcatgc aagatccgtg gaacgatcag gtggcatggt
    28261 ggggtggggg ctccgatgcc ggcggtgcct acttgtggcg tttgcaaaaa agtggtgcat
    28321 tcatttcagg tgtgaaacag gcaaaaaact tttcagttgc cggcggtggt tctatttcag
    28381 caatcagcta ttctcctatt gaccccaatt attggtattt acttaccagt ggaggaaatt
    28441 tctatcattc ggaagatgcc ggtgaaacct ggtatttaag tagtggattt tcaggtcccg
    28501 gaccgcaata tttttatggt gcaactatcg aaccatctaa atcgcaactt ggagtagtgt
    28561 atatcggtgg aagtggttat gataatccgg cagtttatat gtctgacgat catggcgaca
    28621 attttgtgga attacaagat ggcctccctc caacactagt atatgatctg gctgttgatc
    28681 aaaccgacag cctgttgttt gcggcaacgg aagttgcacc ttacgtttgt gtacttgcag
    28741 aaggtaaatg gtataatata gcagctaatg atgctccact gcaaacctat tggagcgtcg
    28801 attatgttga tgaaattaat accgttcgct ttggaactta tggcagaggt gcctgggaat
    28861 ttgccataga aaaattacct accataaata ttcatgatgc caatcaggaa gcattaatga
    28921 gcgtgtatcc aaatccggcc tccgaaaaaa ttcaaatcac cattcctgct tatttctcta
    28981 atgctgaggt tactgttgtg aatatgcaag gcgatattgt atataaaaca aaggcaacca
    29041 taaataaaga catgccatat aacattccgg tttctggcct cgcagccggt tcttatgtga
    29101 ttgctgtgaa gcaaggtgaa aagatgtata catcgaagtt tgttagaata taaatggcaa
    29161 taaaaaaacc gcatccgatt agatgcggtt tttttattaa atatgtgtta tagtttataa
    29221 tttttcaaaa ttacttattg ccatttgact accttgtgtt gccctaagcg tatagattcc
    29281 tgcaggcagt gcagaaacat caatggatac agaatttccg taagcagttg atgtaagcac
    29341 tacttcacct tgcagattca atatattgat taccgcatta ttttctgttc cttctaattg
    29401 cagcacattt gcaacagggt ttggtgaaat gcttaaggtg ttttgtaagt catttatgtc
    29461 aacattacca acagttacca tatcgcaata ggtatcagaa catccataag cattataacc
    29521 tgttacacaa acattgtaaa caccggctgt tgcataaata tgtgtcgggt ttgattctgt
    29581 cgatatggtg ccatcaccaa actcccagta ttgcgataca gcagcaccac ttgtttcggt
    29641 gaatgcaaca ctcaaatctg aaatagtaga tgcaaaagag gctgctgccg gttcatcgtc
    29701 tatgagcatg gtaacagtat cgtagataac gcctgactga ggattgtata gataaagaat
    29761 tacactttca aagctctcat caattacatc gtgaatagga acaatgtcaa tagtgccttc
    29821 tgatgtccac gcaggtatgg tgagctgttc gggaattgtt tcataatcaa caccattaat
    29881 tgctgatcct ccgatcatat aatctattac caatggttct gctgatggga tctcatttgt
    29941 gaaagtgaaa tgtcctgatg cacatttttc tacagcaaat gttaaatcat tatctgcaaa
    30001 cacatcggta tgcatgcgaa gacttccaag gcttcctgtt tcaaggaaaa cgccggaatc
    30061 ataaataccg tcagaagcat ctgctatggc cattttcata tggtatgttt cacctgcggt
    30121 tacggctagt ttagcttcaa gaactgttgt taagccatca aattgaatat agtaatcatc
    30181 ggtactatat ggcgtcatat agccatcgcc attttcgata taataatctg aataagttgt
    30241 agcgttgatg ttgttaattg ataccggtaa gtctgtgccc ggaacagtag caatgtttac
    30301 cggcgttggg ataccgggac cgctcaccca aaatgcaaat atgtcgttga accctgaaaa
    30361 tgtgaactca ggatattctt ctgatgcgaa cacatatttc aatttaacgg tatctgccat
    30421 cacggtcata tcaaaggata ttgcacaggc atcgtacgtg agccacatgg taaggccttc
    30481 aagatcagta tctccgggat aatctaaata ggaagaggct gcgccaacat catttggtcc
    30541 tgccgatgag gccgctgttc ccgaggaaag gagaatgcca ttttctatac ccacattcgc
    30601 atccgtgcta ttgtaaaatg cagatgcatc tgtatggcaa tttctctcaa tattagatac
    30661 cactattccc ggtcctgcca gagaataaat aaaggtagta tcatttggct caggtgttaa
    30721 cgttaactgt gcaaaggcac ctgaaaacat caaaatacct gacgtaagga ggagtaattt
    30781 gtttttcatc atcaatgttt agctaataaa gttaacacta catcctcaga aatcagcttt
    30841 ttttctcatt tttaacgcaa tgtcatcgtt taacttcaag cagtttacca ttctgcaaca
    30901 agattcagcc atgaaagttg gggttgatgg cgtgctgtta ggtgcctggg cgttcagtaa
    30961 aggaaaggtt gaacatattc ttgatatcgg ggtcggaacc ggactcattt ccataatgat
    31021 ggctcagcga tttcctgaag ccctgatcac cggggttgaa attgatgaag cggcctgttt
    31081 cgaggcaatt gaaaatgcaa aacgctctcc ctggagtaat cgcattaaaa ttataaatag
    31141 tgattttttg caattggatg aaaagtcatc tgcttatgac ctgataatat ccaatccgcc
    31201 ttattatgaa aagggatggc cggttgaaga tgaagggcgc cgtaaggcaa gggatgctgc
    31261 ttcattaccg cttgtggatt tatttcaacg tgctgcaaaa atttccgggc agcatacggt
    31321 tttttgtctg attctcccgg ccctggcatt ggatacttgc ctgcaaactg ctgcaattaa
    31381 tggttggttt tcaaatcgca ttacaacagt gcttacaaaa cccgagaagc cggcaaagcg
    31441 cgttttaatt gcttttggga gaagtgaatc aggcacttca actgatgtgt tgacaatata
    31501 tgatgctaat ggtaactatt cagaggctta caaaaagttg acaggtgatt tctatttggc
    31561 attttaataa aataaaaaag caccggtttg ccgccggtgc atgatattta gaaacactgt
    31621 tttggtctaa gcttagtatt tatcgtcgaa atctccttcg tcgagattgt aattatcttc
    31681 ctcaccatag tcatcagcgt aatcgtcaag gaattcttca tcatcaaaat catcttcttc
    31741 atcatcagct ttggcgtcat cttcctcatc ctcaaactgt tcgtattcgt gcttgccttc
    31801 gttgaaatag ccatcagcct catccttttc atcgaagtcg aattcatctt tgaagtcctt
    31861 gtcattaaat tgacggaact cctcaaattc atcggagatt tcatttaaat catccttttc
    31921 ctcggtagat tcctcatcat catcacctcc cttggcataa ggcaggttat cgagaccgcg
    31981 aaattttttg tcgtctacat ttttatttag aaactgattg agtttatcaa tgtcggtgtt
    32041 gctggttatc tcaccgaatt cattgatgtt catctcaaat ccatctaaat ctttatgcac
    32101 ttcaggtttg ccttccaatg gctttttctt gggcataggg tactctgatt ttcagcgaaa
    32161 gttatcaaat attttaattg cagtatgcaa acgttgaaca gaaatatttt ttcctaatac
    32221 atccatcatc gcgaaaacag gggggccttg aagtactccg gccagcgcca cacgtaaaag
    32281 cggcatcact tcacccatac caaggttgtt ttctttcata tagtctttta cccccgactc
    32341 tatcgatggc tcagtaaaat tactgataga ttcaatatgg gcgatcaaat ttagaaaaat
    32401 ttcctgcttt tcgggtttcc atttttttct gatggtatcc tgatcatact cgcttatctc
    32461 accaaagaaa aatgtacccg cctcatatag gtcagggatg aaaaccgcgc gctctttcat
    32521 caattcacac accctttcca ggtagtttcg gtcagcttga ataccctttt tatcaagata
    32581 tggttgtaac aggtctgcca gttcactgcc ggttttcctg cgcatgtgct gctggttgaa
    32641 ccacttagct ttttccgggt cgaatctggc accggacttt cctatgcgct caaaactaaa
    32701 tgcggctatc agttcctctt cggtaaatac ctcctgatct gttccggggt tccatcctaa
    32761 aaatgcaaga aagttaatat acgcttcagg taagaatccc ttttccttca agcctgaaat
    32821 attttgttcc ttccagtcta gagaataaag tgggaagcca tatttgtcgc catcgcgttt
    32881 acttaatttg ccattgcctt ccggttttag aattaggggt aaatgcgcaa aggatggcat
    32941 cacctcttcc caaccaaaca tgcggtatgt cattacatgc gtaggggcgc ttggtaacca
    33001 ctcctcacca cgcacaacat gtgtaacctg cataagataa tcatcaacaa cattggctaa
    33061 atgatacgta ggcataccat cgctcttgaa taataccttg tcatccaatt gcgacgaatt
    33121 aaaacttacc caaccgcgaa taaggtcgtg gaattttact tcctcatttc ttggcatttt
    33181 tatacgtatc acataaggat ctccattctg aattcttcgc tcagtttcat ccttcgacaa
    33241 agcaacagaa tttttcatgt actgacgggt gatgtgatca tatgccggag aaggatttcc
    33301 ctgctccatc atgcggttgc gcattgcctc aagttcttca ggtgtatcga atgcatagta
    33361 ggcatgacct ttttcaatca gctccatggc atattttcga tacatgtctt ttcgctcgct
    33421 ctgacgataa ggtgcaaatg gtcctccgat atgtacacct tcatcaaatg ttattcccgc
    33481 ccatgcgcaa ctgttgatca catattcttc agctcccggc acataacgtg tctggtctgt
    33541 gtcttcgata cgtaagatta acttgccacc gagcttcttc acaaacaaat aattatacaa
    33601 cgccgtgcga attcctccca ggtgttgtgc tcctgtcgga cttggtgcat agcgcaatct
    33661 tattggtcta tccatactgt tgtaatgtga tgcaaagtta atcggcttgg tgaaattaca
    33721 ctaatgtgtg tttccgtctt aaaccatgta tagtgaatac tgtcaaatca aactaccttt
    33781 gtacaatgta ttttgtgaag acaccctggt acctgaaaag tgtatacccc agcttggtat
    33841 gggatatgcc caaagataac aggctgttcc tcaccttcga tgatggccct gttcccggtg
    33901 ttaccgataa ggtgttgggc atactttcga aatatgatgt gaaagcgact tttttttgca
    33961 tcggcgataa tgtcagaaaa catccggaac tctatcaaca aattttgcag gaaggtcaca
    34021 tgacaggaaa tcataccttt catcatgtca atggctggaa aacagatacc gatgcttata
    34081 tcgaagaggt atcagcctgc agtaacttag ttaattcgca attgttccgg ccgccatatg
    34141 gtagaatcgg ttaccagcaa attcaggaat tgaaacggca atatgccatt attatgtggg
    34201 atgtattgag tggtgatttt gacacacaac tgaaaccgca agattgcata gaccatgtaa
    34261 ttcaacacgc tgccgatgga tctatcattg tatttcacga cagtgaaaaa gctgccgata
    34321 gggtgttgcc ggcattacct gtggtgatag aacattttct cgaaaaagga tatcgctttg
    34381 aatccctgcc tgcaaacact gcatcgttac ctcgcggcct ccatttgtca tgatttgaat
    34441 accttagcgt tgtgcccgaa ttaatagata cgcataccca catttattta cctgaatttg
    34501 attcggattc agacgacatg atccaacgtg cacttcaatc aggtgtttcg cacatgatga
    34561 tgcccaatat cgatttagaa tctgtagatg ccatgatggc ccttgcgaag aaaaatcgcg
    34621 catgcaagcc aatgatggga cttcatccat gttcggtaaa gaacgatgca gacgatgtta
    34681 tgcttaagat acatcaattg ctccttgaca aacaatatca atggtatgga atcggcgaat
    34741 gtggtcttga ttattattgg gatctcactt ttgtggatca acaaaaggct gctttcgtta
    34801 ctcaaataag atgggcgaag gaaatgcagc ttcctctcat catccattcc agaaatgcca
    34861 ctgatgattg catcgatctt gtaagcaaac ataaagacgc aaaccttcgc ggcatttttc
    34921 attgcttctc aggaacagaa ttgcaactgc aacaatctat cgatcttgga ttttatattg
    34981 gaataggtgg tgtggtaacc tataaaaacg gagggctcga taaagtgcta aaaccggaac
    35041 atcttcctca ccttgtattg gaaaccgatg ctccatattt atctcctgtt ccatttagag
    35101 gaaagcggaa tgaaccgtct tatttagtgc aaattgtgaa taaactcgct gaaattcttg
    35161 gcctgcctgt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn catgatccct
    35281 tgagatgggg gcttcaaata aataccatgc agccattcgc tgcgctcaat tgatattttt
    35341 attggaagga gggacgaggg attatttgca tgatcccttg aaatgggggg cttcaaataa
    35401 ataccatgca gccattcgct gcgctcatct gcatttttta tttcaaaatg ctgcgccaag
    35461 taaacttgtc acatcctttt gaaataaaaa atgcagcctg cggtaggctg cattggattt
    35521 ttttggcgga gagagaggga ttcgaaccct cgataccctt taggggtata cacactttcc
    35581 aggcgtgctc cttcaaccac tcggacatct ctccggctga aaggatggca aaggtacgac
    35641 ttccggggct tattctaaag attaatcgaa tcaacgatgg atctggatat tgtatttgag
    35701 ctcgtatttg ttactcaatt cagattgcca gttgtcgggt tttaagcgct cgccggcaat
    35761 aaacatgatg ctaagtctgt ttgttcccat atccaatgca tcgccgtcgc tgataaacaa
    35821 tgtggcactt tttcctgttt ttatggtgcc atatttttcg tcaataccgg ctagcatagc
    35881 attgttcagc gtaatcattt ttagggcttc ttctctattt aaaccatatg ccgcagcagt
    35941 gccggcctga aaaggaagat tgcgcaaatt ccagtaagaa tcaccttcat tgcataccga
    36001 aagtccgcac ataatgccgg catcatgcaa cattgcgggc aacttatatg gcaaatcaac
    36061 cggatcgcca tcataggaag gtaattcatg tgttgcctcc aataaaacag gaatatttct
    36121 ttctttcaat aattcagtaa cacgataact ttgccttcca cccctgatta caattttaat
    36181 atgaaaagaa tcactcaacg caacagcacc aataatctgt tttgcactgt ttgcagatat
    36241 aaataatttt tgttttccgt tcaacacttt tcgcattgct tcaaaccgca aattgtgctc
    36301 gcgaggtgca ttgccattgc aatatgatct tgcctcagca aaaaatgtat atatgtcctc
    36361 tacctcgctt cgatattgcg cattagggcc ataggtgttt ttttcatagt cgtaattata
    36421 caccgatggc catgacatat gcatgttgtc gtcggttgca atggcagcgt cttcccaatt
    36481 ccaaccgctc attttaaata cactgctttg tccgcctatt acaccaccgt aaggaacaac
    36541 ttgtgcaagc aaaacaccat tcgtgcgcac agtcggaata acctttgaat ctgtattgta
    36601 agctattaac gatctgatat ttgggttgta cacgccaact tcataagcgt ctcgtgtcga
    36661 cctgacagca gtaatttcat tgaggcctat tgttgagccc atgcctataa atcccggata
    36721 aatatccttg cccgttgcgt cgatcaatga tgccgtattt gcgtcatagc cgctttcctt
    36781 catcggacca gcatactgaa tactgccgtt ttgaatgata actagcccat tttcaataac
    36841 tgtgccatca ccaacatgta tgcgtcctcc ggaaatagct acgggaacct gttgtgcatg
    36901 agttgcaact atatgcagca aaaatgctat ggtcaataat tgtttaatat atcgagtcat
    36961 aataatttct ttttttactt gttattcgcc tttcatgaag tcaattacat cttcgcagtc
    37021 atattcgcga tgtggtgtta caactatcgg aacagtgatc gcgccatttg ttttttctgc
    37081 catcatttta ttgatgatgc gatgacgctc tgcatcttct gcagcaatta attgatcttg
    37141 tatacttctg tcatataaaa ttttaccgtc aactattgtt ttttctgcca tggcataaac
    37201 actcaatgga tcttcactcc aaatcacaat gtcggcatcc ttgcctactt taatgctgcc
    37261 taccttgtca tctacatgta gcaatttcgc aggattaata gttaccattt tcaaggcctc
    37321 cggttccgac atgttgaagt atttcactga tttcgctgcc tcctgattta acctgcggcc
    37381 catctctgcg tcatcagaat ttatagcggt aactaatcct gcatcatgca tgattccggg
    37441 attttgtggg attgcctcat acacttcaaa tttgtatgcc caccagtcgc taaatgtaga
    37501 ggctcctgca ccgtgaattt tcatcttgtc ggcaaccttg taaccttcaa gaatatgagt
    37561 gaaggtattt atcgtaaagc caaaagaatc tgcaacatgc atcaacatat ttatctcgct
    37621 ttgcacataa ctgtgacaag taataaatcg ctttccttcc agtatttcgg caattgcttc
    37681 caactccaga tctgtgcgca ccgattgtgg gttttctttt ttcgccttgg catattctga
    37741 agctctggta aatatatcga catacacttg ttcaacaccc atccttgttt gcgggaagcg
    37801 cactgaataa tcatcacccc aattgctttg tttaacattt tcaccaagag cgaattttat
    37861 aaaacccggc cattgcgcga atttcatgtc ctcaggtgca gcaccccacc gcaatttgat
    37921 aagttgtgtt tgcccaccta ccggatttgc tgagccatgc aatatatgtg cacttgttac
    37981 acctccactc agttgtcgat atatattgat atcatcacaa ttaattacat cagcaatacg
    38041 cacttcagca ctgctgtttt gtgtcccttc attcacgcca tcagtaacag caatatgtga
    38101 gtgctcatca ataattcccg gtgtaacatg tttacccgtg ccatcaataa tacgcgcatt
    38161 ttttgcactg atgttttttc ctacctgcaa aatttttcca ttgccaatta atacatctgt
    38221 attttttaaa acgccctctt tctcactagt ccagactgtt gcattggtta tcaaaatatt
    38281 tccctgtgta ggtgctgctt cccacccata agcagtaaac ggatacataa cagttccaat
    38341 ttgtacagta tcttttttct gaggtgtcgg cttttcagca tacacgcttg caaattcggc
    38401 tttccaattg atccatttat cattcgcatt gccttgtccc tctatattct tcccatcgtg
    38461 gtatccggtt agtcgatata atgtgccgtt atctgcaaat tgtatctgta tgttttcagt
    38521 tgaaatggct ccttgcgctt tgagggtgtc tgtatttttt attatcgaaa attcgggagc
    38581 atttatttta ccttgaatta atagtccgta aaccatatta tcaatatgca aagcataggc
    38641 acctcgcaaa tcactatttt ctgaatttac gacataaggc aaaccgttta cccaatttgt
    38701 gtaaatgata gcgccttcgc ggaaaatatt atctgagctg ataaaaaaat cagcgcgttt
    38761 gccttttgca agtgatccgg tttcgctttc taactttaaa atttgtgcag gaacagatgt
    38821 caatgccgac aatgccgttt tttctgacaa accatactgt aatgctttgc gcacatttgg
    38881 cagaaattgt gatttatcat cgagacctga actggtaagt gcaaatgtaa ttcctgcttg
    38941 ctcggtaatt gccggatttg ccggggccat ttcccaatgt tttaaatcac tgatacttaa
    39001 taattctgca tcccaggggt cgctcacatc aggtgttttg ggaaaattga gcggcaatac
    39061 caaaacagca cctgtttgct tgatttcatt gatgcgttga tattcattgc cgctgcccat
    39121 cataatgaaa ttatacccga attctttccc gattttagct acacgcaaaa tttcaagttt
    39181 ctcatcggta tcaaaaaagt gaatcagatt tttttgatcc aacactgctt gcaatgatat
    39241 attctgttct gaattttttc caaccctgcc ataccagtcc gcatcgtaaa atgtctgtct
    39301 tatcaatgca atacttccca tctcagactg cggatattct tgtgtcgatg tgcctttgcg
    39361 aaaggaataa tgatcgctga tatttggctt aactaccatc tcacctgcac gctcatcgcc
    39421 caacgataca agtgcaccgg ttccgcgcat gataccatca tgtcgatgtg ttaacaccag
    39481 tccaaaacca ctgcttcgcc ataatttagc aacatcaccc tgaggtttga atccggaaac
    39541 ggcctgatat tcaggagtta ttgcctgatt ccaacttact gcctgctgat tgctgtttaa
    39601 catttgcgga cctctgccta cctgtggatt ttcctttgca ggcattccgt accctgcatc
    39661 cagatctata aaggccggat aaatgtactt gccatgcagg tcttcaacaa cggcattttt
    39721 gggaataact actgctgtgc caacatcaat aatacgaccg tcgctgatca gcaaggtcgc
    39781 atcctgtaag gtattgcccg gatcagtaac aatagtggca tgcgtaaaag cataggttcg
    39841 tgatcgctca tcataaacac cattaacagg aaaagtggtc tgcccgtaaa tgatgcttgc
    39901 accaagtata cttagtgcgg taagtaagat tttctgcata ggtagattcg gttaattggg
    39961 taatgcccgt tccgctaaga taatcaggtg tatcaaatcc ctactagtgc cgttaagtaa
    40021 ctttaacagt aaagggcctg ttttcctttt tcaataaacc ctggttgatt tgggctgtct
    40081 aaatctcaaa gaatgacacc ggcgtgaaca ttcgcagtat tttcagcgta tctttattac
    40141 cctttatatg tttcgtaaat acctgccatt gatcctagcg ctcgccttgt gcaacaaggt
    40201 gtttgccttt catatcattg gtggtgaaat gatttataag cgactcacgg gaaatgattt
    40261 tgagataaca ctgaaaatct accgcgattg ctctaatccc gaagcagcac ctttcgacga
    40321 tccactgcaa atttatgtat ataatgcgtc aggcattctt gtcgatagtc tggtattatt
    40381 ttttcccggt tccgataata ttgatcccga aattgtaaat ccttgcatga ccgttgctcc
    40441 ggatctctgt gtgcaggaag caatttatgt cgggaatatt aatctgcctc caagtattgg
    40501 tggttatgat ctggtgtatc aacgctgttg caggaatagt accatcatca atatttatga
    40561 tcctattcaa acaggtacta cctataccga acatattccc gatccgggaa cattaattaa
    40621 ttcaagtccg gagtttaata atcttccacc aatagcatta tgtgccggat atccgtttgc
    40681 attcagccac gcagcaacag atcctgatgg cgatgcctta gtctataaat tttttacacc
    40741 ttacaacgga gcatcctatg atcttccgga tcctgcgcct gcatctccac caccatttaa
    40801 tcatgtaatt tttgtcccgc catttaatga gtcttatcaa attgcatcat cacccgcatt
    40861 tacgctggga agcactacag gatggcttgc aggaacgcct tccgacctcg gtcaattcgt
    40921 tgttggcgta gcagttgaag aatatcgtgg aggcgtgtta atcggcacgc attaccgcga
    40981 ttttcaattt aatgttaccg attgtacacc tgatattgtg gcatctgcac ctcctgaaat
    41041 aaataattgt accgacttca ccgtattatt cgataacgaa agtattggca ctgatgattt
    41101 ttattgggac ttcggtgttc cgggtgtaac tgatgataat agcactgaag ataatcctgt
    41161 ttacacttat ccggatacag gaacatacac tgtaatgttg atcgcatttc ccggaacgga
    41221 ttgttctgat actactttta caacagtgca catttatccg caattaattc ccggcatcag
    41281 ttttgaaaat acttgcgcag gttcaccggt aaattttatg gatatcagta ctactgattt
    41341 tgggactatc acatcatggc aatggaatta tggcgatgga tggggcagct cagaacaaaa
    41401 tccggtttat agttatgagg aaccgggaaa ttatatgctg atctttacag tggaaaatag
    41461 tgttggctgc attgccgaaa tttatgacac cattactatt tatcacctcc cctttgcaga
    41521 tgtcgcaacc gatagcgcat gtctgcatac ctcaggtacc gtttttgacg catcggtagt
    41581 gctggcagga aatgatattg tcgactggca atgggaaatg gaacctgatc aatattttag
    41641 tggagaatcc ttcgattatt attttgatac cgcaggtgta tatcttatca atcttacagt
    41701 aacaactgat aaaggttgtg tcgacagcat tctggatacc atcatagtaa acccgcaggt
    41761 agtggccgga ttattgaacg ataccgtgat ttgcgaaggt gatagtgttc aggttttcgc
    41821 ctcgggagga acatattatg catgggaacc gccttccggt gttagcgacc cctctagccc
    41881 tgaaccctgg ctctcaccag atgaaagcac ggtttatggc gttatcgtta ccgacggttg
    41941 cacttcagac acggccttca tgcaggtcga aatactgcct gccccacatg tgattgccgg
    42001 tcctgatacg atagtatacc acggtcagga ggtggcaatg tatgccaacg gtgcctcatc
    42061 tttcaactgg gagcctcctg atggcctcag cgacccgttc agccccaatc ctatcgctct
    42121 gccggatgtt actaccctct atttagtcac aggaaccgct gagaacggct gtatttcaac
    42181 agatacagcc ttggtttata tcatccctaa ctgcttcaac ttcgctactg tcaacgcttt
    42241 tagtccaaat gacgatgggg tcaatgacag gtttagattt attatagcag gtgatgacgg
    42301 gctcgtttct atggaaatct acaaccgttg gggtaagatg atctttaata ccaacgatat
    42361 tgaagcgggc tgggacggaa ccgacggaaa tgggcatccg caagagcttg gcagttatat
    42421 ctatgtcatt tatacgtcat gtgatggtaa ggaacagcgt ctaagcggta gtgttacctt
    42481 gttgagataa gttcgattac ttatctttaa caccctaagc caattttcac atgaagcgaa
    42541 ccaggttact cctcctctcc ctgatgacaa ttattggtct gaaggcacac gcatcgcata
    42601 tcattggtgg cgaaatatcg tacacatgta tcggtacaaa tcaatatcag gttgtgttga
    42661 ttttgtatcg cgattgtaca agtttcacgc catttgataa tccggcatac atgacaattt
    42721 atactcagga tggcgattat gtaaataact tcccattgta ttcaccgttt attaccggaa
    42781 taccggcgga atctgataac ccttgtcttg atgctcctga tgatgtatgt gttgagcaag
    42841 caatttatac tgctatcatc accgttcccg atggtgatca gggttattta ttggtgtttc
    42901 aacgctgctg cagaaatgca ggtatcgtaa acatcgaaac tcctgatgat atcggtgcaa
    42961 cctatacaca ggaaattcca ccacaaacag atgctacttg taacacatca ccggtgttca
    43021 acgcatttcc tcctacagta atttgtatcg acgacccaat tgttttcgat cactctgcaa
    43081 ctgatatcga tggcgattca ttagcttacg atttctattg gccgctgcaa ggtgcttcat
    43141 ctttcgatcc ggctcccgta attgccgctc cacctccata tgatggtgtg gtatgggtta
    43201 caggatatga tgaattatat ccacttgatg cagtacccgc atttgcaata gatcctgtta
    43261 caggtatcct tacaggcact gcaacagcag aaggtcgata tgttgtgggt gtgcgatgca
    43321 aagaatatcg cgatggcgtt tggctcggag atcatatccg cgatttccaa ttcaacgttg
    43381 ttacatgtac accaactgtt gtcgcacaaa tggatgtggg agatgcaact tttgatgaca
    43441 ccactcctga tgatgaatat ttaaattgtt ctgatttcac tgtaatattt gataattatt
    43501 caatcggtgg aactgaattt ttctgggatt ttggcgatgg cagtacttca acacttgaag
    43561 atccaacaca cgtatatgca gataccggca cttacaccgt tatgctgatc gttaaccccg
    43621 gatttgtttg tgccgatacc gcgataggta taattaaatt atacaataca ctcactgcag
    43681 cttacgatta ttctgcaggt tgctcaggca cgcctgtaac atttgaagat aattctaact
    43741 caacagaagc aggcgatatc attacttggg attggaattt tggcgatggc gcaacttcca
    43801 ctactgctaa tccggaacac gaatatgctg atggcggaac atataccgta gtcttaactg
    43861 ttactaccga tgtcggctgc cagtcatcag tgtcagttga tattcttgta gaaccgggtc
    43921 ctgatgtcga cttcctggct cctgatgttt gtcaggatga agaagccgaa ttcaccaacc
    43981 tcacaacaat tacaacagga tctatcagca gctatagctg ggattttggc gacggagcaa
    44041 ccagcactga agatgagcct tctcatcaat atgatgctcc cggaacatat accgttactc
    44101 ttatagctta cagtgctaac ggatgctcag ataccctcga acaagatatc tatattggta
    44161 tgcttcccga tgcttatgca ggtgttgatg atactattat gtatctcgaa acctacacat
    44221 taaacggcac cggtaccggc gatttcgttt gggatccgca aaatccgata gtaccaccgg
    44281 catcaatatc aagccttctt gtagctaatc ctactataga actagctgaa actactacgt
    44341 ttgtgttaac cgtcaccagt cccgatggtt gctcgcaaac tgatgaagtg accatctacg
    44401 ttgaagacta tccaattgtg gaagtgccaa acgccttttc acctaacggt gatggaacaa
    44461 atgatgtcat tagcgccttt ttacacgata tttctgaact tgaacagttt accatcttta
    44521 atcgatgggg acaagtggtg tatacttctg atatcattaa tgacgcttgg gatggcatga
    44581 tagacggtaa ggaagctgaa atgggcactt acgtttattt ggtagtagct aaagatacaa
    44641 acggcgatat gatcacccgt cagggaaata ttacgctggt tagataaaat ttggtcgcat
    44701 aaacgacccc gaataagtag ctttgcaatc ggaagacaac cgatgaaaaa gctacttatt
    44761 ttttttgcgg tagcggcaac aattgctagc tgtactactg cctcctcatc cgatcaggat
    44821 actgctactg ctgagccgac agcatcaccc ggtatgctcg tgtataccaa gcattgtaag
    44881 ctttgtcatg gcgctaaagg agatctcggc ctcagcggcg ctgctaacct gagcatcacc
    44941 ttgttatcgc atgatgaaat tacacatgtg gtaactgaag gaagaaaagg tatgccttcc
    45001 tggaaagcgc aactttctcc gcaggaaatt caggaagtga cagattatgt actcacactg
    45061 aaaaaataat gtcccccgag agaaaaacat atactgcaat tggtgtaatg tcgggcagtt
    45121 cgctcgatgg ggtagatatt gcgctttgtg aaattaaact ggaagctcaa tcttcatttg
    45181 aaatcattgc ggctgaaacg tataaatacg ccaatcagga aatcagctat attaagtcat
    45241 tgcttcctgt cattaccgat gatcatcatg cggctcatac tttttttgca gaaatatttg
    45301 cacgacatat tacgcagttt ttgaaactat ttcccgatcg ccaaattgat tttattgcct
    45361 tacacggtca caccttattg cattttcctg cgcagggtgt gagtcttcaa ctcggtgatc
    45421 cgcaattgat ggcaaatatt ttgggaattc ctgttattgc acatttcaga caagcagata
    45481 ttgatgcagg cggacaagga gcaccattag tacctatgtg cgatgttatt ttctttaagc
    45541 aatatgatgc ttgtcttaat ttaggaggta ttgccaatat ttcttttgaa tcggctagcg
    45601 gtcgcattgg ttttgatgtc tgtgcctgta atcagttgtt aaattattgt gctgcgatgg
    45661 caggtctcga ttacgatcca gaaggtgagc tcgcagcagc aggaatgatt gatcaggatt
    45721 tactcgatca attaaatgct gtaccatatc ttgatatgcc ctggccaaaa tctttagata
    45781 atcattttgt tcatcgcgca tttatcgata ccattgccaa aagcactatt cctttaaatg
    45841 ataaattagc taccgctgta gaacatattg ctatcgctat tgctgctgtt attattgcgc
    45901 aaccggagcg acgtcctttg catcaatatc aattattggt taccggaggc ggcgcttata
    45961 atacatttct gatagaacgc attgcggcta atgcaggaat aaaaatcaca ctgccgggcg
    46021 ataaaattat tcagtataaa gaagcacttg caatggcact tatgggtgct ttacgcatac
    46081 aatcgaaacc aaattttctt caatcagtaa caggtgctaa aacagccgta tcgggcggtg
    46141 taatcgcata tcctgaaaaa ataatcgcac ctttaccatc atgatagttg ttaccggggc
    46201 caatggactt gtgggttctt atctctgcag atggttgctg cagcaaggta aacaagtgcg
    46261 tggcatgcgc agagccgata gcaatatgcg attggttgaa gatatagctt ctcaaataga
    46321 atgggtgatt ggtgatgtta ctgatgtggt tgccattgaa aatgcgatgt ccggagtgga
    46381 tcaggtctat cattgcgcag cagtcatttc atacagaaaa aagagtgccg cacaaatgat
    46441 gcgcatcaat ggcgaaggca ctgctaatat ggtaaatatt gcgctcgatt tgggtatcaa
    46501 aaaatttgtg catatcagct caattgctgc gctcggaaga acaggaaaag atggtgaaat
    46561 aattactgaa gaagcgccgt gggatatgaa atatattacc accgattatg ctatcagcaa
    46621 atttactgcc gaacgcgaag tatggcgcgc tatggctgaa ggtctaaagg cagttataat
    46681 taatccaagc ataatccttg gcgcaggcga ttgggaaaat ggatcgagta aattattctc
    46741 aactatttat aagggattta aattttatac caccggaatc accggatatg tagatgtgcg
    46801 tgatgtggta cgcatagcac aagtggccat ggacagtgac atcactgaac aacgatttat
    46861 tgttaatggc gataattgga cgaataaaga tgtgctacaa cacatggcaa ggtcattgca
    46921 tgtaaaggag ccttccgtgc acgctggaaa aacattatcc gaaattgcat ggcgtatgga
    46981 atggttaaaa ggactattca gcgggaaaga acctgctgtt acaaagcaaa cggcaagaat
    47041 tgcaaatgca actacttact ttaacagcaa taaaatgaaa gacacttttc attattcctg
    47101 tgtaccctta gaaaaaacaa ttgctgatac cgcagcggca tttctggctg aaaaaactga
    47161 cggtaaattt catcctctgg aattttaata tgcaaaacat acatcatatt ttatttgatc
    47221 tggataatac tttgtgggat ttttcgggaa actctaaacg catactcgca caaatacata
    47281 atgatctttc gcttgatcaa tatggcattc ccgatttcca tcgctttcat gagcaatatg
    47341 cttttcgaaa tgaatatttg tggcatcaat acgcactggg aaatgtgaca cgcgatgaag
    47401 tgcgactaaa cagattctac atcaccttca acgatttcgg tctcgataat tatgtgctgg
    47461 caaataaagc tgccgatttt tacattcatc atacccgaaa acaaacggac cttttgccct
    47521 atacaagaga attgcttgat catctacacg gtaaatatgg cttgcatatt attacaaacg
    47581 gtttcgatga agtacaattt tttaaactgg aaaacaccgg aatcagacat tactttcaaa
    47641 cggttacaac tgctgaaaat gcacaaagct taaagccgaa taagaaaata tttgatcacg
    47701 ctttaagtct tattcccgct aaagcatcgg aatgtttata tattggcgac agccccgatg
    47761 tcgatggcaa aggttcaata gatgctggga tgtcttttat ttggtttaat cctgcaaaga
    47821 aagaaaataa agaaggctat cgtgaagtgc agcatttgag tgaaattgct gctttactct
    47881 gacattattg aacaaaaaaa agaccgcaat gaaaattgca gtctcctcta taaattatgt
    47941 atcctgttat ttcttaggat cctcagtttt ttcagctggt tccggttttg cagcactcac
    48001 cccaatgctc aactgatggc aagatccggc atcggtggct tttatttctc ctgttgtctc
    48061 aaccgctgca gcttctcctg tagtggtaac ttcagttgaa acgccggctc cttcggaagt
    48121 tgcggtggtt gttgctccgg aacgacttac accatgactg cagcatgatc ctgttccggt
    48181 gcatttaatg tttccttgct cagcttcaac agctttgatt accggtgcgg cagttgtcac
    48241 ttgttctgtg gcagtaacag ttgttgtagt cgtagcggta gttgctgttt gagccatggc
    48301 aacagtaaca ccaaaggccg ttactaatgc gaaagagagc agttttttca tgatattaag
    48361 ttgtgattat atactaaaga tataaaaaaa tcaattgtga cgcatcacgc gagctaaaac
    48421 tgtttgaccg ccttttactt tttcgccgat tttcacttca acctttgaat ctaaaggcag
    48481 gaataaatcc acacgtgagc cgaatttaat aaagccgaat tcggaacctt gtgtcacagc
    48541 atcaccttca tcaacataaa atttgattct tcttgctacg gtacctgcaa tctggcgcat
    48601 cagaatttct acattatcat cctcatcctc aataacaaag gtggtgcgct cgttttcggt
    48661 agaactcttt ggatgccaag ccagcaaata ttttccggga tggtatttaa agtattttac
    48721 ctgtccactg ataggatttc tgttcacatg cacatttttc acgctcatga aaatggaaac
    48781 ctgcaaacgg cggtctttaa aatattctcc ctcataggtc tcttcaacaa ctacaacctt
    48841 tccatcggca ggcgctatga tagagccttc ttcaatgaac agactgcgct tcggattgcg
    48901 gaaaaacgac atcacaaaga tgatgcctcc caacgatgct atcaatacta aggtattgaa
    48961 cagcctggct tcgggcataa aatagtgcgt gcagaagttg aggatcacta aaaacaccaa
    49021 aaaacctatg atgatcttgc gaccttcttt atgtataaaa cgtgtaaatt tcatttcttt
    49081 gaatgagcta tagcccgcaa aaataacact ttttgggcgt tttgggttca tgagccacct
    49141 gctacctgat gaaatcgatg tagaagacag cagccggaac tgcaaacatg aagccgtcga
    49201 agcggtccag aaatccgcca tgaccgggca acagactccc ggaatccttg atttgaacgc
    49261 ttcgttttaa catcgactca accagatcgc caaaagtgcc ggctaccgaa actaccaggc
    49321 ctagcactat ccattgtgtt gcactgatgt attccgggta aaatctgctg agcacaaacc
    49381 cacatgccat ggtgcatagc aatccaccaa taaagccttc ccaggttttt tttggtgaaa
    49441 tggactcgaa caacttgttt ttaccgaaaa gtcttcccgc gaagtaagca aaagtatcgt
    49501 tgctccacac taagcacaac aaaccaagtg caaaagcttg ctgaaagtta ccggctccat
    49561 aattcatgta caggccaaaa ggaaatacca catataacaa tcctaataca gttagtccga
    49621 cctgctgaaa aggaaattcg cgtttgagat ataattcaac tattagcgta atcggtatga
    49681 ttaggagtag gagctcaatc aaaacatcat tttgaatata attatatagt agcccattta
    49741 ttattcctgt ttgcaatgtc attatcatta tcccaaaggc aagcggtagt cccatcacaa
    49801 tccacttgcc gcgagcagga gtagctgaag tagttctcac atattccaga tgtgtcagga
    49861 aacctatcag cataaaaacg gcataaaagg ctgttcgatc aacaaaaatg cttccaataa
    49921 taatgactac tcccacaaga gcagttataa tgcgtaaaac gagattgttc attcaggtaa
    49981 attataaatt aattccagag cttcctctgc ttgtttatcg tgtacgtata attcgaattt
    50041 caccggcatc acatcaccac caaaaggcat gggtggtttt tcaaataata cggcatagat
    50101 ggcatgctgc tgcaattcac tcatcagata gttcgcctcc atcctgtcgt acgtcgagta
    50161 cacctgcatc cagtcttttt ccatgcgatt ccttgttgcg atataggtac agcaataaaa
    50221 atacaaaagg tgctgcaatc aatccaaacc aaatattact tgattcggtg atatctcctt
    50281 gtattactcc gcgatgtgtc agaaaaattg cc
//
LOCUS       DAOQHY010000003        47233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_275_length_47233_cov_6.064331, whole genome shotgun sequence.
ACCESSION   DAOQHY010000003 DAOQHY010000000
VERSION     DAOQHY010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 47233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47233
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_275_length_47233_cov_6.064331"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            369..1718
                     /locus_tag="PLL28_00505"
     CDS             369..1718
                     /locus_tag="PLL28_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67817.1"
                     /translation="MKLKALTLAVACYSCIMIGHSQVPLAAFIGQESLPADSDPICTI
                     PIYPEVSTNLEGPLGGELVHDFKLYTLEGDSVQLSGVLNDGKPVLLVGCNYTCYVFRG
                     KMDVVNDMQAIYGDQINIFLVYTVEAHPVIDFSPYFGYENVGSDNYAEGILYRQPTTY
                     GERKDIVSDMLDAMDIDVPVLIDGPCNEFWAAYGTNPNPAWLIDPNGTVFDAQKWFNK
                     EPDNMYASIDSMLNLVGTGVFTPEGTFTAEDESEEVYYGIVDNTIAAHITLTNTSDDD
                     VLIDFIRADESIPDGWFTAMCTDLCYSPDIDSTTVFLAPGETEIVRVDFFTDLIPSSG
                     YVEINCTNHYQPDNTYSFTLNAASVAETAINNVDGLSSFLTSPNPSMAGNPVTIQYNN
                     TASSATYTLYNAAGMLIGNGNLQLGSSHVIDIDQPAGIYQLLISEEMPVAMATIVVQ"
     gene            1743..3284
                     /locus_tag="PLL28_00510"
     CDS             1743..3284
                     /locus_tag="PLL28_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNF67818.1"
                     /translation="MFRSIQWIAATVIAFTISACALDVPGYLDITPYTYSSVDSTGGE
                     WEPVYVHGADLVSIPTPESVTSDAYLTEIEELKSIANNLTSEQSNAIEYWGNNTTIRW
                     IEITEELIAKYNLAPSPDENGEYGVPDQVFPATYPQFPFAHPPYASRAYAYLSAAIYD
                     ALIATWEYKYTYDRRAPYMVDETISPAYPKTSIPSYPAEDAVIASVAEDVLEFLFPLE
                     TAYISNMAYDCRNSRKWASMNVQSDIDAGDSLGHALADIFIGRAKTDSMKFAQVLQEE
                     YDEMEAAANALWSWPNWENLEVPQRPIGITPKFGHVVPWWVDDITTVRPGPPPAIGSE
                     AYETARQELLDFSKDLTDEQERIAYFWGDGFGTYTPAGHWNTIAKEPIIDHAYNPLRT
                     ARVFAYLNTAMMDAGISCWDTKYYYMYPRPPQADPEIKTLFGLPNFPSYTSGHSTFSA
                     AAATVLSYMFPENEVLYNQYMHEASESRIYSRIHFRFDCEAGLTAGTTIGNLAVDAAK
                     ADGSE"
     gene            complement(3397..3951)
                     /locus_tag="PLL28_00515"
     CDS             complement(3397..3951)
                     /locus_tag="PLL28_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnA domain-containing protein"
                     /protein_id="HNF67819.1"
                     /translation="MSISITLQTRCAGVCELCGAEPAVIEITVSPKDESTENQVAICT
                     VCETALANADEKNHWQCLSGSIWSTEPAVQALSYRILYGIRDESWAAEILDSGAADES
                     VINWAMKAYEVADVHQDAYGNVLANGDNVILTQALKIKGTSFSAPKGTQVRSIHLVHD
                     NTEQIEGKINGTMIVILTKFVKKG"
     gene            4044..4379
                     /locus_tag="PLL28_00520"
     CDS             4044..4379
                     /locus_tag="PLL28_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67820.1"
                     /translation="MKSTSLTLLAMMILFGCEKYNPAANTPECVADSIKDFSKDAWCE
                     DIKIEEYLFQGGYVYCYDPGTCGNDMDTKVVDANCNVLGYLGGFSGNDIINGESFTSA
                     QFIRTVWEQ"
     gene            4393..4770
                     /locus_tag="PLL28_00525"
     CDS             4393..4770
                     /locus_tag="PLL28_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNF67821.1"
                     /translation="MNLNQVTVTVSDIHRSIHFYQLLGLRLIVHTHDKYARFLCPSGD
                     STFSLHVPDSPLTPTGTSIYFEVDDVDTKINEFIKKGISIDHMPEDKPWLWRESELHD
                     PDGNHIIIYHAGVNRIDPPWRLK"
     gene            complement(4787..6001)
                     /locus_tag="PLL28_00530"
     CDS             complement(4787..6001)
                     /locus_tag="PLL28_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HNF67822.1"
                     /translation="MARDISISSYTYELPDDRIAQFPLGDRASSKLLWYKDGKIHDYI
                     FSQLPDLLPENVTLIFNTTKVVCARILFSVEGAQKPIEIFVLEPQSNMTMEQAMLQKG
                     RAVWKCLVGNNKSFRADAIKKEIQWHGEQHVLQVNKPQLSGDVFLVEFIWSESLTFSE
                     MLDAAGQIPLPPYMKRGPEQEDSSRYQTVYANEEGSVAAPTAGLHFTDAVINELRRKN
                     IQIEHVTLHVGAGTFRPVKAATMATHDMHAEEISISKETLKHLVNNPDSLIAVGTTSL
                     RTLESLFWIGWKWKHAEQENVPMVNQWDAYDADVPVEFTFVDALNAIISRMEIFGITT
                     ITTKTQLLIAPGYTIRTAKALITNFHQPNSTLLLLVATFVGADWKLIYNHALENEYRF
                     LSYGDSSLLWRK"
     gene            6106..6930
                     /locus_tag="PLL28_00535"
     CDS             6106..6930
                     /locus_tag="PLL28_00535"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNF67823.1"
                     /translation="MSELLAYRDGQYLNKSDIRIGLDDLGFERGFGVFDYMRQHKGII
                     PFLKDHLERLDHSQSVLKFAEPLPMSLVAEVITRLLEANKLDDGYVKVMITGRMQNGI
                     PVPVLTVYQDVMKAYPASSYSDGIKLIISEYDKPYPEYKTTFYLGSFRELARMQSENA
                     DDVLFYVGELVHECSRCNIFYVKGGLIYTPLEHILLGVTRKHVINCALQNFMVIEAIV
                     TMERLFQADEVFISSTTRNIMPVTRIEDRIIGNGKPGYVTQELMQSFNSYLENYSA"
     gene            6927..7505
                     /locus_tag="PLL28_00540"
     CDS             6927..7505
                     /locus_tag="PLL28_00540"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNF67824.1"
                     /translation="MSTTAATIKEIIAYLKEHADPSHAEGMKVTAPGAKKIYGVRSPE
                     MNELLKAIKPGSFELVDALWETGIYEARMLAVKLLEWQSKTDPERGIATVIRFSPEID
                     NWAICDTIGMKSLKKLNKKYPERIMSLSEQFAVSSNMWERRLSMVILWDLCKDKKWHP
                     RIKKIIDMHRGDKEKYIKKGVVWLDKTLSDHA"
     gene            complement(7731..8369)
                     /locus_tag="PLL28_00545"
     CDS             complement(7731..8369)
                     /locus_tag="PLL28_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HNF67825.1"
                     /translation="MRNYVAEALGTFAILFFGTGAAIVDSITGGTVSHVGISVTFGFT
                     VMSMIYVFGNTSGAHFNPAVTIAFYFSGRFPGKEILPYVIAQFLGAIAGSGLLLFLFP
                     DAATRGETVPSRDVLQAFVLEGVLTFFLMLTIIHVAHGSKEQGMFAGLAIGSVVLMAA
                     LFAGPICGASMNPARSLAPALMSGNLQSIWIYLTAPVLGAVGAVLGWKWLRR"
     gene            complement(8372..9133)
                     /locus_tag="PLL28_00550"
     CDS             complement(8372..9133)
                     /locus_tag="PLL28_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002981231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNF67826.1"
                     /translation="MKIALFSDIHANLPAMEACFADIDTRRPDAVYCLGDLVGYNIWP
                     NEVIRLIRDRGIPVIFGNYDQGIGLHSDECGCAYKTDIEKEMGKISISFTNSIVGDDE
                     RNYLRSLPAHIRVDHLIQEETMSLLLVHGSPRRVNEYLFEDREEKSMLRIMQQANADI
                     MCFGHTHKPYHRILQDGDRYRHAINIGSVGKPKDGDPRGCYVMLTIDEHTSVHAEDAV
                     QVEFVRFQYDVEKAAHAVEESPLPNAYADMLRKGY"
     gene            complement(9238..9570)
                     /locus_tag="PLL28_00555"
     CDS             complement(9238..9570)
                     /locus_tag="PLL28_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNF67827.1"
                     /translation="MGISKLEDFTVRDNKVSAYAKALSHPARVAILRLLASRSSCMCG
                     DIVDELPLSQSTVSQHLKELKESGLIKGSILGAKVCYCIDPVAWEAAKSYINGLFDDY
                     SPCIDLLC"
     gene            9734..10438
                     /locus_tag="PLL28_00560"
     CDS             9734..10438
                     /locus_tag="PLL28_00560"
                     /inference="COORDINATES: protein motif:HMM:NF021365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2064 domain-containing protein"
                     /protein_id="HNF67828.1"
                     /translation="MDFRHPGHMAFVLFTRTREEEARAKDFSATHSLRTNSLIAGHLI
                     GHAERTARNTGLPCFVISSAQQHGNSFGEKLAHAFEDIYSLGYTQVIAIGNDCPTLTT
                     NDLLKAATALDEHGAVMGPANDGGVYLIGLHRKHFDRLYFEKLPWSSDNLFVSLTEYF
                     SVNGISFATSRALSDIDYPTALQQVLKGTQLSTSLRFILISLLAGLYAVINKDQTNPT
                     LSQPRMYVGLRAPPVF"
     gene            10533..10871
                     /locus_tag="PLL28_00565"
     CDS             10533..10871
                     /locus_tag="PLL28_00565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HNF67829.1"
                     /translation="MERSTYYDPKDLKNFGKISDWDGELGRAFFSYYGKVFEEGALTA
                     REKSLIALAVAHAIQCPYCIDAYTEDTMAKGCDEEQMMEAIHVAAAIRGGASLVHGTQ
                     MMNKFKDLSM"
     gene            11044..12102
                     /gene="arsS"
                     /locus_tag="PLL28_00570"
     CDS             11044..12102
                     /gene="arsS"
                     /locus_tag="PLL28_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_274671343.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM protein
                     ArsS"
                     /protein_id="HNF67830.1"
                     /translation="MKTSKSLHAQHHKLADTNYQLKVLETHEVAGADFERFDDKLKAI
                     GLLPLKPTKLEIFQVNVGYMCNQTCAHCHVDAGPDRKEIMTRETMQQCLDIIAKTDIS
                     TVDLTGGAPEMNPDFRWFVEEITKLGRKTMVRCNLTIIMANKKYHDLPQFFAEHKVEV
                     VSSLPFYNKSRTDAQRGDGVFEDSIKALQMLNEVGYGKEGTGLILNLVYNPGGAFLPG
                     SQLSLEQQFKRELKRNFDIEFNNLFCITNMPISRFLDYLIESGNYESYMQKLIDAFNP
                     VAAAGVMCRNTVSIGWDGFIYDCDFNQMLGLHVNSGAPNHISKWDTYALQSRNIVLNQ
                     HCYGCTAGSGSSCGGAVA"
     gene            12125..12736
                     /locus_tag="PLL28_00575"
     CDS             12125..12736
                     /locus_tag="PLL28_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNF67831.1"
                     /translation="MTQDALIIMIKNPVKGKVKTRLAADIGDDAALAIYMQLLEHTKQ
                     VTEMIPQDKFLFYDHVVERNDIFPNSTYKKYTQCNGDLGVRMDYAFSIPFKNEYKKVV
                     MIGSDCYALTAEHLAKAFEALDHHDFVIGPAHDGGYYLIGMKKWNRWVLENKSWSEHK
                     LFQETQADILSRNGTLHLLEPLSDIDELKDIQAISLFKSLIQA"
     gene            12733..13815
                     /locus_tag="PLL28_00580"
     CDS             12733..13815
                     /locus_tag="PLL28_00580"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNF67832.1"
                     /translation="MIAIIILLAALFLAYSNGANDNFKGVSTLFGSGTTNFKTALRWG
                     TITTLAGCLFAMYIAQGLMKNFSGKGLIPDDTLQDPNLAIAVAIGAAATVFLATRIGI
                     PVSTTHAIVGALTGAGFIAVGSELNVNQLGETFLKPLILSPFIACGASIILYLLFRQI
                     RTWLRIEKDTTLEIQEPHFAQNSISASTISLTQQPKESYTGHIVGISAQKILDSLHFI
                     SAGAVGFARGMNDAPKIAGLLLLVHFLNVPSMIIIVAAVMAAGGLLNAKKVAETMSRK
                     ITYMNPGQGFTSNLVTAVLVWTASMSGLPVSTTHVSVGGIFGIGVVNRKADYKMIGKI
                     VGSWVLTLPIAAVLAATTFYILQQFN"
     gene            13838..14812
                     /gene="arsM"
                     /locus_tag="PLL28_00585"
     CDS             13838..14812
                     /gene="arsM"
                     /locus_tag="PLL28_00585"
                     /inference="COORDINATES: protein motif:HMM:NF038099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis arsenite
                     methyltransferase ArsM"
                     /protein_id="HNF67833.1"
                     /translation="MSTTYLETTRNVYKEAAENPQKGLCCTTTPVWQLPGLVIPQKML
                     EMNYGCGSTVNPRDLVNDPTILYVGVGGGMELLQFAYFSRRKGGVIGVDVVEEMLQAC
                     HENLLEAEKLNPWFKREFVDLRKGDAFNLPVEDNSIDIAAQNCLFNIFRDEDLEKALK
                     EMYRTMKRHARLVMSDPTCEQEIPENLRNDEELRALCLSGSLPLQEYINRITNIGFGT
                     IEVRARRPYRILDPKHYATNELLYIESAEVCAIKDPMPADGPCVFTGKTAIYFGDEEY
                     FDDHKGHVMTPNQPLAVCDKTAGNLAALNRDDIFISGSTWFYDGGGCC"
     gene            14905..16038
                     /locus_tag="PLL28_00590"
     CDS             14905..16038
                     /locus_tag="PLL28_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNF67834.1"
                     /translation="MENNNWSNNSVFKTTLIVILTLAGLFGVVWLLMQITSILFYFII
                     AAIISIIGKPIADLLDKLHIGKFKMPSSLSALLTIGVLYGILFGIISIFTPLVIQQTN
                     TISHIDTQKVADGLQQQVQVLDSMLVHVQDPTQPAKSVELILNESLREAINATSLTHI
                     FNNILGGLGNVMVALFSITFITFFFLRGERLFYNIIMVLTPTKLEGNGRRILHSSKKY
                     LTRYFVGLIIQSTVVFILIAVGLLLLGFGANSWLIALFAGIINIIPYIGPIIGMLFGM
                     LIGITTNLSVDPNFDIIPMLIQLLILFQGVQLIDNFVSQPIIYSNSIKAHPLEVFFVV
                     LVFGSLAGVPGMIAAMPVYAFFRIIAKEFFSEWKVVRKMTQDI"
     gene            16245..18710
                     /locus_tag="PLL28_00595"
     CDS             16245..18710
                     /locus_tag="PLL28_00595"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HNF67835.1"
                     /translation="MQKTILYATAIALCGWLINTSSIIAQTAVRPVAQMVRDAHAAKI
                     NFTPAQTLDRVSAPAYRSKAEAFANNITYARLDGDAIHQLFEEKQAAISLTVPYKEGQ
                     NLTLELVQVNLFADGFKVTTSESNGAAVEYTPGVYYQGIVQGDDNSIAAISIFENEMI
                     GMVSTSSEGNINIGKYGDGDWQDYLVFSDRDIIVQPENAGCATVDDESYLDTFHQIME
                     NGGGERITKCPKIYFECDYQLYQNKGSVTNTTNYITGVYNNAAIIYTNETVPTQISEI
                     FVWTSADGYATASSYDALNSFKAFRTTFTGDIAHLVTLDPGGLGGIAATINGLCNSNK
                     YCYSDIDATYSAFPTYSWTVMVVTHEMGHLFGSYHTHNCSAWVGGALDNCYTTEGGCP
                     PGPAPTSGGTIMSYCHLTGYGINLSNGFGTQPGNAIRNTITAAACITSCGGVPTYCTS
                     VGSSTADEWIQTISMAGTTNNSGNNSGYADFTATNINLTPGGTASFTLTPGYTGTVYP
                     EYFSIWIDYNKDLDFADAGENVYNSGAVTSAVSGSFSVAAGMTGTTRMRISMKYNALA
                     GPCEVFSYGEVEDYTASFVPVVTYCTSAGTSAASRYIDYVKIGTITRASGNDGGYYNG
                     TAMSANVIKGSSYTLTYSAGFSGTIYQMYWRGWIDFNRDGDFADAGEQVFQKKATSAG
                     NLNKTITIPTTAGTGTTRMRISCKYGGYPTSCETFANGEVEDYTLNIMPGLPSPEENP
                     YYLNIYPNPSEGVFQMEFLNAFNGGITMEVFDMTGNRIRQEVIESDNGWIVLDISDAA
                     PGMYIVKTTLANGTTESKQIIKQ"
     gene            18810..19595
                     /locus_tag="PLL28_00600"
     CDS             18810..19595
                     /locus_tag="PLL28_00600"
                     /inference="COORDINATES: protein motif:HMM:NF016658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HNF67836.1"
                     /translation="MKHNNLIYRTLIGMMLAVVLLPACRTIDGIDSNSKPVSHEQWSA
                     LLDKHVDDNGFVDYKGFILDSTALNDYLQLLEKGYPNEKNWTREEILAYWINAYNAYT
                     VQLIIRNYPIESIKDIKPGVAFINSVWDVQFINIEGQELDLNNIEHNILRKMEEPRIH
                     FAINCASYSCPKLRNSAYTAENLNTQLDLAARDFINDPSRNNIKNGSAQLSSIFNWFT
                     GDFTKNGSLKDFINQYSNNKIAADTPIEFMEYNWKLNEQNGGK"
     gene            19710..21047
                     /locus_tag="PLL28_00605"
     CDS             19710..21047
                     /locus_tag="PLL28_00605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HNF67837.1"
                     /translation="MHHVIIGNGITGITAARTLRKLSKDHITVISGETDLFFSRTALM
                     YIYMGHMRFKDTQPYPKDFFEKNNISLKKAWVSKIDSAKKTLHFDSGDHMQYDNLLIA
                     TGSKSNRFGWPGQDLKGVQGLYSYQDLLELEENTRNGINRAVIVGGGLIGIEMAEMLH
                     ARHYPVTLLVRESNYWSNILPAQESALITNHIQDHGFDLRLNTTLKEIKGDANGRVTG
                     IITDSGEEIPCQVVGLTAGVSPNISVLQGTEIKTDRGVLINKRMQTSVEGVFAAGDCA
                     QFTETLPDRRPVEQVWYTGKIQGECAAKNMLGINAEYDPGYWFNSAKFLDIEYQTYGT
                     VLSKLRDGESEFYWEHNDRQKCVHIVFNSSDHKFIGINTFGIRMRHKLFNTWLQESRS
                     VEYVMAHLRAAQFDPEFYSRYEENIIDAWHAQFPEHRITQPDEKTIRSLIFNA"
     gene            21053..22426
                     /locus_tag="PLL28_00610"
     CDS             21053..22426
                     /locus_tag="PLL28_00610"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNF67838.1"
                     /translation="MADQQIDMSLSGSSPANFNGLQKTGIAITSIGILFFFIAWFGVG
                     NQQPLICLLITLAGFIIGGITYSFGTYGHLPKGIKNNRAFFTSISSRGTLGWISGIVI
                     TAFYIALYWRDYWKSVLHTDVFAGLVSMFDPLSQWMRKGPADQWFVYGSLYTLAVFVM
                     GIKFILKYRHNRYQVIRTISVMFFQFGFAWIIPAVMKALYNFEPYYSYFWPLDYDAFF
                     PNSINYFKDQGNLGVFFLFWGAVMAFIATPILTYFFGKRWYCSWVCGCGGLAETAGDP
                     FRHLSDKSLKAWKIERITIYSVLGAITLITAALLIDNSMHNQLLGSSAKWIAKGYSFA
                     IGMVFSGVIGVGFYPLMGSRVWCRFGCPMAAYMGIIQRFKSRFRITTNGAQCISCGNC
                     STYCEMGIDVRAYAQRGQNIVRASCVGCGVCAAVCPRGVLKLETGPENPSRTEVLFTS
                     DDLKILS"
     gene            22502..23194
                     /locus_tag="PLL28_00615"
     CDS             22502..23194
                     /locus_tag="PLL28_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNF67839.1"
                     /translation="MAKINVIIPVFNESGSIGHVLNDIPAGLVEEVIVVNNGSTDNTA
                     EIARSKGATVLLEARRGYGSACLKGIAHIRRKPVEQQPDIVVFIDGDYSDYPEQMPDL
                     IRPITEQGFEMVIGSREKGTRIKGSMAPQQIFGNWLATSMILWLFGARFSDLGPFRAI
                     TWKALESLEMRDTNYGWTVEMQVKAAKKKLRFTEVPVNYRPRIGVSKVTGTVKGTIMA
                     GYKIITTILKYY"
     gene            23209..24684
                     /locus_tag="PLL28_00620"
     CDS             23209..24684
                     /locus_tag="PLL28_00620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF67840.1"
                     /translation="MSITIIVIYTIAITLLFFYCIMQLSLAIHYIRYKRRMEAEEATF
                     KGPESEYPMVTVQLPVYNELYVVERLIDAVAAFDYPKDKLEIQLLDDSTDESFEIARK
                     KVDFYKQQGINIVQVKRPIRKGFKAGALEYGLQTAKGEYVAIFDADFVPYPDFLKKTI
                     PYFLTDEKIGVVQARWEHINKDYSILTKMQAFALDMHFTIEQQGRNAAGYFINFNGTA
                     GIWRRTAIDDAGGWNSDTLTEDLDLSYRAQLKGWKFKYVEDVVAPAELPTDMNALKSQ
                     QFRWNKGGAETARKIGRRVMGAELPIRIKLHAIAHLFNTTNYIFILITAILSVPLLFI
                     KNHYIDFNYFKYASIFLMGSIAIAYAYYISTLQREKTVGQTVKVYISRFPVFLAITMG
                     MSLHNAWAVFRGWLGQQTAFVRTPKFAINNSGDIWKKKKYVASRVSPITYLEGIFSLY
                     FLSGIIMGIYYQDYGLLPFHIMAFMGFALIFYFSFKHARSN"
     gene            24695..26041
                     /locus_tag="PLL28_00625"
     CDS             24695..26041
                     /locus_tag="PLL28_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67841.1"
                     /translation="MENRTTRTWQIAAYVLYGFLCTILLYFTSRENFIQLIAIYAGMF
                     ALYFLLARSSERTNYLGEIIIAGILLRLIATFALPNLTDDHFRFLWDGQLVTHGFNPY
                     ASIPEQVRAEGYFGISTLDDHLFNSLNSAQYISIYPPIAQAIYAIGALIGGSNWDIQI
                     LVIKIILLAFECGTIFLLPKVLKQLHINVGHAAWYILNPLIIVELSGNMHLESTMVFF
                     SLLSIYYFNKNNLWLSAISFGLAIATKLWPLMLMPLLFRQIGFKQTMRYSVIAGVTAI
                     IFLLPMLIEFSKITGSFNLYFQQFEFNASIFYIGKYFFDKDVNYEAFSNMRGSLPFIA
                     AFLILVFSAVYKKQYWYAGMLIAFSIYLLLATTVHPWYLAPLILLSVLSGLRYPILWS
                     LLIYLSYYTYITPAYIENMTFVWIEYVMVIGFAAYELIHSPMIESKIITSRLPFKI"
     gene            complement(26024..26797)
                     /locus_tag="PLL28_00630"
     CDS             complement(26024..26797)
                     /locus_tag="PLL28_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF67842.1"
                     /translation="MKLTLLWILICLTHLCYAKNALSDSVKFNSYRVTVDINENQVIF
                     SFFPLNYIICQVYFDPDSLLLDDDNDGVYNTIDLEPNSDDWAAVDIHGVTLDSDHDGC
                     PDHLDEEPYSSPVLPMENCVNVSIGCIWDVGVSGREYVEHAYTTWCLPYIFFDPAEAV
                     IRPDAVPSLNQIADILIRYDKLTLRVVGYSDSTSDEGVNVKLSADRAGEVVNYLHSKG
                     VPESRLIVVYKGETSDHIKNEKSVVEHQLYRRVELYFER"
     gene            complement(26857..27642)
                     /locus_tag="PLL28_00635"
     CDS             complement(26857..27642)
                     /locus_tag="PLL28_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF67843.1"
                     /translation="MLLRCKILALVFVVYLLNPVHAQNNDNESKAHKHRRLFHRQVIC
                     VYYPNPLSYLYDFPAYTSELDTMDSDMDGVVNRFDKEPNTPALSPVDIHGASLDSDRD
                     GCIDAEDPEPYSDVFDSMVDCHNVYKEIPLTIESCAHLRLDNQLSGADDWILPFIFFD
                     AHKADIRPDAEAALTMIAEIMIRYPMLSIRAYGFSAGGVEKDSSALLVERRIISSVNY
                     LIELGVPEDRISVVKYRKPKQIFYDGDIVEGFPRIVEFWIESN"
     gene            complement(27767..29374)
                     /locus_tag="PLL28_00640"
     CDS             complement(27767..29374)
                     /locus_tag="PLL28_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF67844.1"
                     /translation="MISRKLTIAIVLLYSGLTFAQTSSFPPKPTDQWQIGLNLGLPII
                     QGDLRAEPFGGNHAPAFGVGLNVRKSLGYIVSLRLHGMYAQTYGMDYVQGSYAYNKTL
                     NGAFYDAINVDGQPAENYYSTGANYTNNVWVPNYKTQFGHGSLDFIVNLNNLNFHQHD
                     PRFYAYAYAGVGAMAYNTKYDALDADGNPYPFQTILEGFGSQNTSQKERLDALHAALD
                     GEYETQAEVANPAARGADEVRATWVRNPSISGGFGVSMRIGSKMELGLDQRATHYFDD
                     LADGQRWQYDGTYSPHYDLLTYTSLNFGYNLGKAESIDPMWMVNPLAVLYDQMAEIDP
                     DNMLKDTDNDGVIDRLDKEPNTPEGTPVDTHGVSLDSDKDGCPDSEDPEPFSSPVLPM
                     ENCQNVHLTQEWVESYVDQRLSKYTPGASTDWILPYIFFDLGKSNIRPDAVPALNQVA
                     DLMKRYDKLSVEVVGHTDTRASEEFNMKLSEDRARNAINYLTSKGVPESRLKIVYKGE
                     TENLIKDANEEAEHQMNRRVEFHIIKN"
     gene            complement(29707..31569)
                     /locus_tag="PLL28_00645"
     CDS             complement(29707..31569)
                     /locus_tag="PLL28_00645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNF67845.1"
                     /translation="MKKCTLLLLLAGILSTGYSQSITAYPDTTICSADSVMLYAVVDG
                     SYGTESYTYAEIPYAPEPIGGTVHNMVDDTHTGPFSIGFDFCFFGQVYTQFYIASNGW
                     ISFLTPTGGMDVNWTPDGPIPFGAGVTPKPAIFGPWTDWHSGLCTNCIFHEVVGVAPN
                     RKLIVTYEDVPLFSCTAFEGTFQFVLHETTNVIENHLTHVDVCPTWDLGVAVQGIINE
                     DGTEAFSVAGRNATDWFADDESWRWIPDAITWYETATGIVVGTGDSIYVNPDVTTTYT
                     AEITLCDGTIATDEVTVTVSTPYEVTLDVQQIACFGATNAWIDVDVTGNTNPITYTWS
                     TGSTIDSIYDLGAGTYTITIQEEDGCAWEEDVVITEPPLLTLDTVLTVDVTCFGGSDG
                     VVNLNPGGGVLPYQFSFDGINWQTDSNFISLEAGTYTFTVRDAFGCTTSFTGVVISQP
                     PQIMVDAGPDQSIAYGQSVVLLGTTGAPVVTAISWSPVDYLSCIDCLQPTCTPTFNST
                     YVLTVTDANGCQASDSVNIWVDIDFVVPNAFSPNDDGLNDVFNISTDLLISYDIRIYD
                     RWGAEVFHSTDINFGWDGKLGGLPQEIGTYVYVIKSTTTLNTPITKSGTITLLR"
     gene            complement(31622..32290)
                     /locus_tag="PLL28_00650"
     CDS             complement(31622..32290)
                     /locus_tag="PLL28_00650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HNF67846.1"
                     /translation="MSQIQKSTLKFLKDLAAHNDRVWFEDNRTIYEAAKGNFIDFTAA
                     VLEKTASFEPAMAGIDAKKAVFRIYRDVRFSKDKNPYKTNFGASLGMGPKSGLSAGYY
                     LQIQPGNASFAAAGAYMPELPVLAAMRQEIDYNLKDFEKILKAASFKKYYEGLDEIEV
                     LKTTPKGYSPDNQALPYLRHKSYVVSRAFSDKEVTDPSFLKEVVNTLKSAHPLVQFLN
                     KAGS"
     gene            32377..34665
                     /locus_tag="PLL28_00655"
     CDS             32377..34665
                     /locus_tag="PLL28_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67847.1"
                     /translation="MNCKRVILKAVFSVITISSSMLRTGMAQDEFHAEGVKTYNYGTF
                     NTAVSDTPKELFSLTPDSLYTHPEFGILPYNAPCEDCIELLQYRTQNSRMFVKKGTNG
                     SKFYSQGAYQDLNYKDNKGNWVTIDPTLQPSTALPGVYLGLHQTNPTTLNMVEGYTAI
                     AMQDGAELRFNQNISAYATSEIGLNIPGIAINRSVYSVGADGAAVKDAFPGIDQELRF
                     NVAEIKSGYTVWNLNAIDTSKKYFVIRDEFDLPEGYTISTDQYEGTFNVNGFWFGDLV
                     IENADGFEMARMKAPVMYDSNPNDTLDFVIPMDIIGYTVKQNGRTVTLKLVADMAYLR
                     DPQRQFPITIDPTVYGTTTTWTGTSGTDDSPTFCNVVVNVPTPANATLTGSSVHWEFD
                     ADGVACSPFCKLKFLQLRIGTSCGYSPSAAGVWVCPACNTAGTWNPTVDDATTAALVS
                     CFTPQCASFNIAFTIYHNQYQCVTPGGCVTTCAHLDLATITVEGETVTTTGLAAGATA
                     YTVLDCTLQSGWLSTTTPNYGVPPYTYTWSPGGYTTSPVYVTFPMGTTTYTVTMTDAC
                     GNVATDNVTVINNCVTLPIELISFSGYSLQDVNILEWEASSSLENKTFFVERSSNGRD
                     YVSIGMVEAANPWQTKYSFSDDQIASNVYYYRLKQVDEDGTTDYSNIISIQSSGNTDA
                     LVINEYDANTGNLALTLFSPYTGSLTLHVFDITGRLIADYPFQTQAGATSIQLQLPSI
                     AAGTYLLSIQETGVNASARLVR"
     gene            34690..36636
                     /locus_tag="PLL28_00660"
     CDS             34690..36636
                     /locus_tag="PLL28_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNF67848.1"
                     /translation="MIAVQNLSISFGGEFLFEDVSFTLKDNDRVGLVGKNGAGKSTLM
                     KILSGLQQPDSGQVILPKDSLIGFLHQDLAKMKGKTVMEEARTAFDEALAFDKRLHEI
                     EHDLTVRTDYESDSYHDLIEEMNEIVHRLEIIDVGSIDKQMELVLMGLGFMREDFNKP
                     VETFSGGWQMRIELAKILLSKPELVLLDEPTNHLDIESIQWLETYLETYPGAIILVSH
                     DRTFLDNVTKRTIEIVSQKIYDYNAAYSTFVELRKERIDQQKAAQKNQEKYVEHTERL
                     IEKFRAKANKAKFAQSLIKKLDKLEEVEVDDEETATIHFYFPKPPRSGRISVEGRGVS
                     KSYGEKEVLRLLDFEIHRGDKIAFVGKNGEGKSTLSKMIAGIEKYTGELTIGHNVHLG
                     YYAQNQAETLSGDDTVFDTIDKVAAGEIRSHIRTLLGAFLFGGEASFKKVKVLSGGEK
                     SRLAMCKLLLEPYNLLVLDEPTNHLDMRSKDVLKNALANFDGTVIVVSHDRDFLKGIT
                     NRVFEFKDKTIKEYIGDIYDYLDAKKISNLAELEMTEKQPKKKEAVPEVVQEQKVQYA
                     DKKQKEKELQRVKKAIEKSEQEIKTLEAEIKTMEATMNSADFYNNSNTADFFTKYNTT
                     KQSHESKMSDWEKLQEELERLNVN"
     gene            complement(36629..38440)
                     /locus_tag="PLL28_00665"
     CDS             complement(36629..38440)
                     /locus_tag="PLL28_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF67849.1"
                     /translation="MKHYLIATLCLTFATQSVSARILGGELWYEQTAADQYIIKLTWY
                     REYEPELEPVFWLGIYNNTDYTLIDTVAAIEIDRSIVDSNYPTCVGMVQKYDIERLDF
                     STPVTLPENPDGYVIVIMGNTRTEEISNILLPGLRGLTLYTTVPGNENNSAPSFNNHP
                     VWYACEDQSLNMDLGASELDGDDLSYALVPALNADYTEPFFQSLPTPPPFSELSYLGA
                     LSPTNPFPSATTIEIDPSTGMLTGLPTTEGVYIITVEVTETRLGIELSKTRRDIILIV
                     EDCTPTMAEVDLGSEIYQCNDTHVFFECETEPWAGYYWDFGDGIIQTWDIYEHTYPAL
                     GDYELMYIANYDLPCADTFYLPVHIRELSEDVLSTDLLGNPENCFYDTIQLIGEPGFV
                     AEWQDVNTGLIIGTGSPLPVTQFSGDLLATAYYYDDDFCFASDTVSAYFFPDNTPLVK
                     INQDNPQWFLYCDNAFDPFVETLQWFKDDMPIPGAINPNLPLTSCGSYYARITDTHAC
                     VFESEATYYCNSEIEGAETLFFEVSPNPSSGSFSLTLPNTDPVTIRIYNNLGEKVYQI
                     ESAGNAVININTESLAGLYHVIIAGETYVKELPVMIN"
     gene            38563..38877
                     /locus_tag="PLL28_00670"
     CDS             38563..38877
                     /locus_tag="PLL28_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNF67850.1"
                     /translation="MAYTKRTEFSEDEMQLADIAKALSHPARIAILKVLSDGQCICGD
                     IVDRLPLAQSTVSQHLKELKSAGLIKGEIDGPRVCYCLNEPVIGKARKHFNFLFTSFN
                     CC"
     gene            38877..39311
                     /gene="arsN2"
                     /locus_tag="PLL28_00675"
     CDS             38877..39311
                     /gene="arsN2"
                     /locus_tag="PLL28_00675"
                     /inference="COORDINATES: protein motif:HMM:NF040501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance N-acetyltransferase ArsN2"
                     /protein_id="HNF67851.1"
                     /translation="MQISKAHSTDFANIHALLKANDLPVEGVNEVQGAYYLLKNGEKL
                     MAVAGIEGSAPTALLRSVAVDVQMHNRGIGTQFMIDILSLANEQGFRQIYLLTETASK
                     FYERLGFTIEDRSQAPESIRKSSEFTSMCPTSATLMKYIIIK"
     gene            39322..40161
                     /locus_tag="PLL28_00680"
     CDS             39322..40161
                     /locus_tag="PLL28_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HNF67852.1"
                     /translation="MQNDQSIKDMVKDRYTEIATQSKSTNASSCCGAGGCSTVDLQIM
                     SEDYTSLDGYLADADLGLGCGIPTQFAQIKAGDTVVDLGSGAGNDAFVARSIVGEDGR
                     VIGIDMTQVMVDLARNNAQKLNLSNVEFRLGDIENMPLTANKADVVISNCVLNLVPNK
                     EKAFQEMYRVIKTGGHFSVSDIVIVGQLPTELLQEAEMYAGCIAGAIQKDTYLDIIHK
                     AGFSDVRIMKEKAIDLPDDILSNYLSADQIASWRNSGTGIYSITVFGVKAKTCGCGDN
                     CCS"
     gene            40184..40348
                     /locus_tag="PLL28_00685"
     CDS             40184..40348
                     /locus_tag="PLL28_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67853.1"
                     /translation="MDKGDFIQVLLVASVFMVIILLIYSFLNWPVWVSLLLTVISAIG
                     AYLAGRLIKL"
     gene            40386..40976
                     /locus_tag="PLL28_00690"
     CDS             40386..40976
                     /locus_tag="PLL28_00690"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HNF67854.1"
                     /translation="MITTGKFATILLAGSLFLGACENSSQDAIKEKRKEEMTNKENRV
                     SPPATATANIGENTVTINYSSPSVKGRQIWNALVPYNEVWRTGANEATTISFTQDVTI
                     LGQPLAKDTYSLFTIPGEGEWVVIFNFNETQWGAFKYDQSEDALRVNVKPTMTETLTE
                     SLKFEVIAGENAGSGIIRLNWEKLQLDIPFENVATK"
     gene            complement(41029..41421)
                     /locus_tag="PLL28_00695"
     CDS             complement(41029..41421)
                     /locus_tag="PLL28_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67855.1"
                     /translation="MKRCLPVMLLSCMLAVVSCDEEPIYEDLNGSENLNRTIKFDVSH
                     VYATSPYSDSTVAGATIKIYAQFEDLLTDYYPDASRITDSLGHAEINGLDEDMYYIKC
                     THPTLGELIDSVTTPAYTTSFVELIYFM"
     gene            complement(41561..43102)
                     /locus_tag="PLL28_00700"
     CDS             complement(41561..43102)
                     /locus_tag="PLL28_00700"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNF67856.1"
                     /translation="MIANDAEKFKKVVSHAKEYGFVFPSSEIYDGLSAVYDYGPYGAL
                     LKNNIKEYWWKAMVQMHENIVGLDAAILMHPTTWKASGHVDAFSDPLIDNKDSKRRYR
                     ADNLIEEYLAKQDDKINKEVEKARKRFENFDEAMFRSTNGRVLEITQKREEVHARFVK
                     AMQDGDLTEMKQIIIDCDIVDPESGSRNWTDVRQFNLMFSTKLGSLAEDADIIYMRPE
                     TAQGIFVNFLNVQKTTRQKIPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQFFVRP
                     GEELKVYDYWKQTRMKWHRALGTDAAMLRFHDHDKLAHYANAAVDIEFNFPFGFKELE
                     GIHSRTDYDLGRHSEFSGKKMQYFDSELNESYVPYVVETSIGCDRMFLAMLCNAYDEE
                     QVNDGMRSVLRLPAALAPVKVAVLPLMKKDGLPEKALEIFNDLRYRHQCYYEEKDSIG
                     KRYRRMDAIGTPYCITIDHQTLTDNTVTIRERDSMEQVRIHIEDVAKVVSEKVDMNNV
                     LRTLV"
     gene            complement(43219..44535)
                     /locus_tag="PLL28_00705"
     CDS             complement(43219..44535)
                     /locus_tag="PLL28_00705"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNF67857.1"
                     /translation="MVCIALSVSLSLQAQTQDKNALQNKYNKLQDEIKDAEELLDNTK
                     QKKENSLNELKLLNRKIDMREQVINNIAAQVDGVNAELTTTNKAITAMQADIVELKSQ
                     YAKMVYYTYVNDQDYKPLHFIFSAESINDAFQRVHYVRSFHGYRKDQIGAIEEISESL
                     QNKIAQIEKEKNEKESLLTKEEQQKKKLDDEKAKKDQAVKKLQTQEKDLKKQIEKKKK
                     DAVGLKNKIQAIIAEEIKKEKERAAQEAAKQKELEAKKLAESNKTGTTPTTNSGSTSN
                     AKTTNAEKPAAEKVNLGLTPEMQLISKNFEGNKGKLPWPVERGTITERFGKHEHPVLK
                     DVIVENNGIDIATAEGATVRSIFDGEVVNVIFNPSFQKGVIIKHGEFYSVYTNLSDVT
                     VKAGDKVSTKQKIGIAWEDPEEGKTEVHLEIWKNTVILDPSLWISK"
     gene            complement(44570..45322)
                     /locus_tag="PLL28_00710"
     CDS             complement(44570..45322)
                     /locus_tag="PLL28_00710"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HNF67858.1"
                     /translation="MNKNAYFILLIATFSLSACGTSHALFSGKIKPMTAEELDKELRS
                     QLIQFETFSSKAKIDLQSNQGNQSFSASIDIRRDSLIGISLRFLGVEGARIRITPDSI
                     EILDRLNQEYLPRDYTFLRDSLNLDLNFSDLQNLIIGNPVMYDSTTLETVESEEFYIL
                     NASKGVYKNQLSMAADFSIVRMFIQDLYARRNLTLLYAEYDKVNGKRFALNRNIKLDA
                     REDLTADVVFTNVEFDAPFEFSFSVNSKYKVN"
     gene            complement(45315..47084)
                     /locus_tag="PLL28_00715"
     CDS             complement(45315..47084)
                     /locus_tag="PLL28_00715"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF67859.1"
                     /translation="MRKKLRLQIGLCALTLMFLAACNGPKEAQQQTGSVVNVGSSASR
                     PKKGIDTAELERQYIDACKFVALEDYNKAVDIFNNIVKIDPNNAPAMYQLGKIFANHG
                     QIGDALAYSEKAASIDPGNRDYQQQYAGILQYSGQGRKAVDVYLGMIKNEIGDEDTYY
                     QLAATYEKLGDRQQAVSTLLDLHKIIGDDEQVMFEIQRLYAAVEQYNNAISWLQKLIK
                     QNPDNTLYLRYLSDYYDRNGQPELAEKTFNDLLAIDPSNTDFQFRKASMQQKAGEYAD
                     YVNTMNNAFANPDGNIDTKIFYLVLFIDSIGRPEFKTKDLVFNWSKLLVEAHPDDAKS
                     YAMRGDFLYYDEQLQSAANVYNKSIALRNDVYDVWLKLFYIYSDLHKSDSLALVSNMA
                     IELYPNQPLSYYFAGIAANQQDKFEDAVKVLRRGLPLTISNIDLRSGMFSALGDAYNE
                     LKKFPESDDSFENALNLNPNDVYALNNYAYYLSLRKEKLERAAELAKKATTLQPENPS
                     FLDTYGWVLYQQGDYKNAAAWIEKAMQKGGDKSGTVIEHYGDIQYRLGNEAKAMEYWK
                     KAKAAGDASDMLDKKINDNKLYE"
BASE COUNT        13990 a         9979 c         9695 g        13569 t
ORIGIN      
        1 tcgccttttt cacgcagagc cgcagagtcg cagagataaa catgcacgta ttcccgcacc
       61 tgctaacttg ctaacctcgc ctcacccctc tccatctcct accgtcgata gagagggagc
      121 taatttcact tcggaattaa attttattaa acaatataat tgcattccct attaacgcgc
      181 cagcgttaaa agcactccac attggctaat cggctaatca atttatgttc attccccaac
      241 acaaagcctc aattccaatc ctttcgttaa tttccaacta aaagtattgt aaactcctta
      301 aaagtacttt aaaccctact gctactaagc attgaaagcc atttctttga gcctcaattc
      361 tatcaggaat gaaactcaaa gccctcacac tggcagtcgc ctgctatagc tgcatcatga
      421 tcggacactc acaagtgcct ctcgcagctt ttatcggaca ggaatcgctt cctgcagata
      481 gtgacccgat atgcacgatc cctatttatc cggaagtatc gacaaacctc gaaggaccac
      541 ttggaggtga acttgtacac gattttaaat tatatactct tgaaggcgat tcagtgcagc
      601 tttcaggtgt actgaacgat gggaagccag tactgttggt aggatgcaat tacacctgtt
      661 atgtgtttcg cggcaaaatg gatgttgtaa acgatatgca ggctatctat ggcgatcaaa
      721 tcaatatctt tttggtttac accgttgagg cacatcccgt tattgatttc agtccgtatt
      781 tcggttatga aaatgttggt tccgacaatt atgccgaagg tattttatat cgacagccaa
      841 caacctatgg cgaacgcaaa gatatcgtta gcgatatgct tgatgccatg gatatcgatg
      901 ttccggtatt gatcgacgga ccatgcaatg aattttgggc tgcttatggc accaatccaa
      961 atcctgcctg gctaatcgat ccgaatggaa ctgttttcga cgcacagaaa tggttcaata
     1021 aagagccgga taatatgtat gcatccatcg actccatgct caatttggtt ggcacaggtg
     1081 tatttacccc tgaaggtaca tttactgccg aagatgaaag tgaggaagtg tattatggca
     1141 tagtagataa taccatcgcc gcacatatca cattgaccaa caccagtgat gatgatgtat
     1201 tgatcgattt tattcgcgcc gatgaaagca ttccggacgg atggttcact gcaatgtgca
     1261 ccgatttatg ctattcgccc gatatcgatt caactacagt tttcctggct cccggagaaa
     1321 ctgagatcgt gcgtgtcgat ttttttacag acctgatacc atccagcgga tatgtggaga
     1381 tcaactgtac taatcattat caacctgata atacttattc atttacgctc aatgcagcat
     1441 ctgttgctga aacagcgatc aataatgtcg atggattatc ttctttcctt acatcgccga
     1501 atcctagcat ggccggaaat cctgttacta ttcaatataa caacaccgct tcttcagcaa
     1561 catatacctt atataatgct gcaggcatgc tgattggcaa cggaaatttg caattgggtt
     1621 cgtcgcatgt catagatatt gatcaacctg caggaattta tcaactgttg atctcagaag
     1681 agatgccagt agcaatggct actattgttg tgcaataatg ttaaactata cctgcctgtt
     1741 atatgtttcg ctcaattcaa tggatcgcag ccactgtgat cgcgttcaca atttcagcct
     1801 gcgcacttga tgtgccggga tatctggata ttacacctta cacctatagt tctgttgatt
     1861 caacgggtgg cgaatgggaa ccggtgtatg tgcatggtgc tgatctggta agtattccta
     1921 cacctgaatc agttacatct gatgcctatt taacggaaat agaggaatta aaatcaattg
     1981 ctaataacct cacttcagaa caatcgaatg ctattgaata ttggggaaat aatacaacga
     2041 tcagatggat agaaatcacc gaggagttga tcgcaaaata taatttagca ccatcgcctg
     2101 atgaaaatgg cgaatacggt gttccggatc aggtgtttcc tgctacctac ccccaatttc
     2161 cattcgctca tccgccatat gccagtcgcg cttatgccta tttaagcgct gcgatctatg
     2221 atgctttgat cgccacatgg gaatataagt atacgtatga tcgacgtgca ccttatatgg
     2281 ttgatgaaac aatttcaccg gcatatccta aaacaagcat tccttcctat cccgctgaag
     2341 atgccgtgat agcatctgtt gcagaagacg tgcttgaatt tttatttccg cttgaaactg
     2401 cctatatctc caacatggct tatgattgcc gcaatagcag aaaatgggca tctatgaatg
     2461 ttcaaagcga tattgatgca ggtgattctc ttggtcacgc tctcgcagat atttttatcg
     2521 gtcgcgcgaa aaccgacagt atgaaatttg cacaggtact acaggaagaa tatgatgaga
     2581 tggaagcagc agctaacgct ttatggtctt ggccgaactg ggagaattta gaagtgccgc
     2641 aacgaccaat cggtattact ccgaaattcg gacatgtggt gccatggtgg gttgatgaca
     2701 ttacaactgt acgtccgggt ccgcctcctg ctattggctc tgaagcatat gaaacagcaa
     2761 gacaggaatt gctcgacttc agtaaagatc tcaccgacga gcaagaacgc attgcctatt
     2821 tctggggaga tggatttggc acttacacac ctgcgggaca ctggaataca attgctaaag
     2881 aacctattat cgatcacgcc tataatccac tacgtacggc gcgggtgttc gcttatttaa
     2941 atactgcaat gatggatgcc ggaatcagtt gctgggatac gaagtattat tatatgtatc
     3001 cgcgaccccc acaggctgat cctgagataa aaacattatt cggacttccg aattttccga
     3061 gctatacatc aggtcactct acattttcgg cagctgcggc aacagtatta agttatatgt
     3121 ttccggaaaa tgaggtgctg tataatcaat atatgcacga agcatcagag tcgcgcattt
     3181 attctcgcat tcacttccgt ttcgactgcg aagccgggct cacagccggc actaccattg
     3241 gaaatcttgc agttgatgct gcaaaagcgg atgggtcaga ataaaatgtc tattgatttg
     3301 ggacatattc ccataatata atttatcgta gttttttcac gcaaaggcgc taagaataac
     3361 ctttgcgcct ttgcgcgaaa cacgtcattg atcaacctat ccttttttca caaatttggt
     3421 caagatcaca atcatagttc cattgatctt gccttcaatt tgttctgtat tatcatgcac
     3481 caagtgaata cttctcacct gagttccttt gggagcggaa aaactggtgc cttttatttt
     3541 caatgcctgc gtgagaatta cattgtctcc attcgccaat acatttccat acgcatcctg
     3601 gtgtacatca gcaacttcgt aggctttcat tgcccagtta attactgatt catctgctgc
     3661 tcctgaatca agaatttctg cagcccaact ttcatcgcga attccataaa gtatacgata
     3721 actcaatgcc tgcacggcag gttctgtgct ccatatactt cccgacaaac attgccagtg
     3781 atttttttca tccgcattgg caagcgctgt ttcacatact gtacaaatcg ctacctgatt
     3841 ttcggtactt tcatctttgg gacttactgt tatttcaata actgcaggtt cggcgccaca
     3901 caattcacaa acaccggcgc aacgtgtctg gagggtaatg gatatagaca taatgaatgc
     3961 tgcaaaatta acctaatttt attgagaacg accgttgaat cacaaaatcg acaggatgat
     4021 gtatctttag aaaaacatct gatatgaaat caacatcatt gacactcctc gcgatgatga
     4081 tcttatttgg ttgcgaaaaa tataatcctg cagccaatac tcctgaatgt gttgccgatt
     4141 caattaaaga cttcagtaaa gatgcatggt gcgaggatat caaaattgaa gaatatctct
     4201 ttcaaggcgg atatgtctat tgctacgatc ccggcacttg cggaaatgat atggacacca
     4261 aagtggtaga tgcgaattgt aatgtgttag gatatcttgg tggcttttcg ggaaatgaca
     4321 tcatcaatgg tgaatccttt acaagcgcac aattcattcg caccgtgtgg gaacaataat
     4381 catactaacc tcgtgaattt aaatcaagtc actgttaccg tttccgatat ccataggtcg
     4441 atacattttt atcagctatt gggattacga ctcattgtgc atacgcacga taaatacgca
     4501 cgttttcttt gtccttcagg tgatagcact ttttccttgc atgtaccgga ttcgccgcta
     4561 acaccaacag gcacaagtat ttatttcgaa gtggatgatg tagatacaaa gattaatgaa
     4621 ttcatcaaaa aaggtatctc catcgatcat atgccggaag acaaaccatg gctatggcgt
     4681 gaaagcgaac tgcatgaccc ggatgggaat cacattatca tatatcatgc aggcgtcaat
     4741 cgcattgatc ctccatggcg tttgaaataa gattatgaag aaaaggttat ttacgccaca
     4801 ataaagaact atcgccataa cttaaaaaac gatattcatt ttcaagtgca tgattatata
     4861 ttaatttcca atcagcgcct acaaacgttg ctactaacaa caataaagta ctatttggct
     4921 gatgaaaatt cgtgataagc gcttttgcgg ttcttattgt atatccagga gcaattaata
     4981 gttgagtctt tgttgtgata gttgtaatac caaatatttc catgcgcgaa ataatggcat
     5041 tcaaagcatc aacaaaagta aactcaacag gcacatcagc atcataagca tcccactgat
     5101 taaccatggg aacattttct tgttcagcat gtttccattt ccaaccaatc caaaaaagcg
     5161 attccaatgt acgcaaagag gtggtgccaa ctgcaatgag tgaatctgga ttatttacaa
     5221 gatgtttcaa tgtttctttg ctgatggaaa tttcctctgc atgcatgtcg tgcgttgcca
     5281 tggttgcagc ttttactggt cggaaagtgc cggcacctac atgtaatgta acatgctcaa
     5341 tttgaatatt ttttcttcgc aactcattga taacagcatc cgtaaaatgc aatcccgcag
     5401 taggtgctgc tacagacccc tcttcatttg cataaacagt ttgatatctc gaactatctt
     5461 cctgttccgg tccgcgcttc atataaggag gcaatggaat ctgcccggca gcgtcaagca
     5521 tttccgagaa tgtcaaactt tctgaccata taaattcaac taaaaaaaca tctcccgata
     5581 attgcggctt attcacctgc aaaacatgtt gctcgccatg ccattgaatt tcctttttta
     5641 tagcatcggc gcgaaatgat ttattgttgc caacaagaca tttccagaca gccctgccct
     5701 tttgcaacat agcctgctcc attgtcatat tcgactgcgg ttcgaggacg aatatttcta
     5761 tcggcttttg cgctccttca actgaaaaca atattctcgc gcatactact ttagtagtat
     5821 taaaaatcaa tgtcacattt tcaggtaata agtcaggcaa ctgagagaat atataatcat
     5881 gaatttttcc atccttatac cataacaatt tactgctcgc ccgatcgccg agtggaaact
     5941 gcgcaatacg gtcatcaggc aattcgtatg tgtatgacga tatggaaata tctcttgcca
     6001 tttgttgcaa aggtataaaa tagtggtgcg atggcgatat ttatgaataa tacgattatg
     6061 aatgcctata taatttgata tcacacggaa tagtatcttt ggcatatgag tgaattactt
     6121 gcatacagag acggacaata tttgaataaa tcggatattc gtatagggct cgatgatctg
     6181 ggctttgaac ggggatttgg tgtattcgac tacatgcggc aacacaaagg cattatcccg
     6241 tttcttaaag atcatttaga gcgactcgac cattcacaat ccgtattaaa atttgcagag
     6301 cctttgccga tgtcgttggt ggccgaagta atcactcgct tattagaagc aaataaatta
     6361 gatgacgggt atgttaaagt gatgataaca ggtcgcatgc aaaatggtat tcccgtgcct
     6421 gtgctgactg tgtatcagga tgtcatgaaa gcctatcctg catcttccta ttccgatggc
     6481 ataaaactga ttatttccga atacgataaa ccatatcccg aatataaaac caccttttat
     6541 cttggttcat tcagagagct tgcgcgcatg cagtcggaga acgccgatga tgttttattt
     6601 tatgtcggcg agttagtgca cgaatgttct cgatgcaata ttttttatgt aaaaggagga
     6661 ttgatttaca caccactcga acatattctt ctaggtgtaa ccagaaagca tgtgattaat
     6721 tgtgcacttc aaaactttat ggtaatcgaa gcaattgtta ccatggagcg attatttcaa
     6781 gcagatgaag tatttatttc ttcaacaaca agaaatatca tgcctgtaac tcgcatcgaa
     6841 gatcgtataa tcggcaacgg taaacccggc tatgtaacac aggaattaat gcaatcattt
     6901 aatagttatc tggagaatta ttcagcatga gcactaccgc agccactata aaagaaataa
     6961 ttgcctattt aaaggaacat gctgatccat cacatgcaga aggcatgaaa gtaacggcgc
     7021 ccggagcaaa aaagatctat ggcgtgcgca gtcctgaaat gaacgaactg ctcaaagcaa
     7081 taaaacccgg cagttttgaa ttggtcgatg cattatggga aacaggcatt tatgaagcgc
     7141 gcatgctggc ggttaaattg cttgaatggc aatcgaaaac agaccccgaa cgcggcatcg
     7201 caactgtaat acgcttcagt ccggaaatag ataattgggc gatttgtgat accattggta
     7261 tgaagagttt aaaaaaactc aacaaaaaat atcccgaacg catcatgagc ctttcagaac
     7321 aatttgcggt ttcttcaaat atgtgggaac gcagattatc gatggtcata ctttgggatc
     7381 tctgtaaaga caaaaaatgg catccgcgca tcaaaaagat cattgatatg catcgcggcg
     7441 ataaagagaa gtatattaaa aaaggtgtcg tgtggctgga taaaacgctt tcggatcatg
     7501 cgtgatcaac gtatctagat gcaagtttct agacttggta tcaggcttgc ctcgccgata
     7561 ggcgggtatc aggtaaaaat tcacgagtca cgaatcacga ttcacgatgc tgtagtgagt
     7621 agtgggtatt gagtagtgag actaatgtaa ttattcgctc gcaaagcgag cgaatttgca
     7681 ctcaccactc accacccgcc tatcggcgag gcaagctcac cactcaccac tcaccgcctc
     7741 aaccacttcc accccaacac tgcgccaacg gctccgagta cgggggcagt aaggtatatc
     7801 cagatgcttt gcaaatttcc tgacatgagt gcaggagcca gtgaacgtgc gggattcatg
     7861 gatgcaccgc aaatgggacc tgcaaataat gctgccatta atactacaga gccaattgcc
     7921 aatccggcga acatgccctg ttccttagaa ccatgtgcaa catgaataat ggtgagcatg
     7981 agaaaaaatg tgaggacacc ttccaaaaca aatgcttgca aaacatctcg agaaggaaca
     8041 gtttcacctc tggtagcggc atcaggaaaa aggaataata gcaatccgct acccgcaatc
     8101 gcaccgagga attgtgcaat cacataaggc aatatttctt tcccgggaaa tctgcctgaa
     8161 aaataaaatg caatggtcac ggcaggatta aaatgtgcgc cggatgtatt tccaaacaca
     8221 taaatcatcg acatcaccgt aaatccaaag gtgacagaaa taccaacatg cgaaacggtg
     8281 cctccggtta tgctatctac tatagccgct ccggttccaa aaaataaaat ggcaaatgtg
     8341 ccaagtgctt cagctacata atttctcata ttcagtatcc ttttctcaac atatctgcat
     8401 aggcatttgg cagcggactt tcttccaccg catgtgctgc tttttctaca tcatattgaa
     8461 accgcacaaa ttcaacctga acagcatctt cagcatgaac tgaagtatgc tcgtcgatgg
     8521 taagcattac atagcagcca cgcggatccc catcttttgg ctttccaaca gatccgatat
     8581 taatggcgtg acgataacgg tctccatcct gtaaaatgcg atgatatggt ttatgtgtat
     8641 gtccaaaaca catgatatcg gcattcgcct gttgcatgat gcgtaacatg cttttctctt
     8701 cacggtcttc aaataaatat tcattcacac gacgcggact gccatgtacc aataacaaac
     8761 tcattgtctc ttcctgtatt aaatgatcta ctctgatatg cgcaggaagc gaacgcagat
     8821 aattccgctc atcatctcca acaatactat tggtaaatga aatggaaatc tttcccattt
     8881 ctttttcaat atccgtttta taggcacatc cacattcatc gctgtgtaat cctatcccct
     8941 ggtcgtaatt tccaaagatg accggaatac cgcgatctcg aattaagcga attacctcat
     9001 tgggccagat attatatcca acgagatcac ctaaacaata aactgcatcc ggcctgcgag
     9061 tatcaatatc agcaaagcaa gcctccatag caggaagatt ggcgtgaatg tctgagaaca
     9121 aagcaatttt catgttgtga aatggttagc gtctttttac cggttaatca aataaaattg
     9181 agttcatggt ttggccgggg aatcatatcg ttatataacg atgatatttt tattcattta
     9241 acataataga tctatgcagg ggctataatc gtcgaacagt ccatttatat agcttttagc
     9301 tgcctcccag gctaccggat ctatgcagta gcatactttg gcacccaaga tggatccctt
     9361 gatcaatccg gattctttta actccttaag atgctgggaa acggtgcttt gcgatagcgg
     9421 aagttcatct acgatatcgc cacacatgca ggaactcctg ctggccaata gccgaagaat
     9481 agctactctg gcgggatgcg acaaagcctt ggcataagca gagaccttgt tatctcgcac
     9541 agtgaaatcc tcaagtttac taatacccat aggtaaaata attatcgcaa tattacgatt
     9601 attttcttct cgacacaatt tgacaatatt atggttgtat taacaattat ttagggaaag
     9661 gtcgcttgac aggtatccta atttctctac tttagccgaa ctattgaccc ttaggggttg
     9721 ttaagttggt tccatggatt tcaggcatcc cggacatatg gcttttgtgt tgtttacgcg
     9781 cacccgcgag gaggaagcac gtgcaaaaga tttttctgcc acacacagcc ttcgcacaaa
     9841 tagcctgatc gccggtcacc tgatcggtca cgcagaacga actgccagaa atactggtct
     9901 tccctgcttt gttatcagca gcgcccaaca acatggtaac agcttcggtg aaaaactggc
     9961 tcatgctttt gaggacattt acagcctggg ttatactcag gtaattgcca taggtaacga
    10021 ctgtcccacg ctcaccacca acgacctcct gaaagccgca acagcactcg atgagcacgg
    10081 cgcagttatg ggaccggcaa atgatggcgg tgtatacctt atcggattgc atcgcaaaca
    10141 tttcgatagg ctgtattttg aaaaattacc atggtcatca gacaatttgt ttgtatcgct
    10201 cacggaatat ttttcagtaa acggcatatc atttgccacc agcagagcat tatctgatat
    10261 cgattatcct actgcactgc aacaagtgct caaaggaacg cagctttcca catcactgcg
    10321 tttcatatta atttccctgc tcgcaggttt atatgccgtg atcaataaag atcaaacaaa
    10381 tcctacactg tctcaacctc gtatgtatgt aggtttaagg gcgcctccgg tattttgaaa
    10441 tgtttcttta caatttatcg gcgcacatac attaatcgta tgtcgtaatt cattttttat
    10501 tttattcaat ttataagacc aataatatca ttatggaaag aagtacctac tacgacccga
    10561 aagaccttaa aaattttgga aaaatatcag attgggatgg cgaattaggc cgcgcattct
    10621 tttcttatta cggaaaagta tttgaagaag gtgcattaac agctcgcgaa aaatctttaa
    10681 tcgcacttgc tgttgcgcat gccattcaat gcccatactg tatcgatgct tatacggaag
    10741 atacaatggc aaaaggttgt gacgaagaac aaatgatgga agccatacat gttgctgcag
    10801 ctatccgcgg tggcgcatca ttggtacatg gcacacagat gatgaataag ttcaaagacc
    10861 tatccatgta gttagtgggt gagtttacct cgccggtagg cgggtgggtc agttaaacac
    10921 aacacaccgc aacacaccac attacattac atcacatcac atcacaccac accataccaa
    10981 tccctagcat ggcactaaaa ctcacaaacc accaaaaaag ctccagaggg gcgacgttat
    11041 agtatgaaga cctcaaaatc attacacgcg cagcatcata aacttgctga caccaattac
    11101 cagctaaaag tgctcgaaac acatgaggtt gcgggtgccg actttgagcg gttcgatgac
    11161 aagttaaagg ctattggttt gctgccgcta aagcctacaa agcttgaaat attccaagtg
    11221 aacgttggct atatgtgtaa tcaaacttgt gcgcattgtc acgtggatgc aggcccggat
    11281 cgaaaagaaa tcatgacgcg tgaaacaatg cagcaatgcc tggatattat tgcaaaaacg
    11341 gatatttcta ctgtcgattt aaccggcgga gctccggaaa tgaatcctga tttcagatgg
    11401 tttgtcgaag agatcactaa gctcggaaga aaaacaatgg tgcgttgcaa tcttaccatt
    11461 atcatggcaa ataaaaaata ccatgatctt ccacaatttt ttgccgaaca taaagttgaa
    11521 gtggtttcgt cattgccgtt ttacaataaa tcgcgcaccg atgcacaacg tggtgatggc
    11581 gttttcgaag acagcataaa agcacttcag atgctcaacg aagtcggtta cggtaaagaa
    11641 ggcaccgggt tgattttaaa cctggtttat aatccgggag gcgcctttct acccggctct
    11701 caactctctt tagagcaaca gttcaaacgc gaattgaaac gcaatttcga tattgaattc
    11761 aacaatttgt tttgtataac aaatatgcct atctctcgtt tcctcgatta tctgatcgaa
    11821 tccggaaact atgaaagcta tatgcaaaaa cttatcgatg ccttcaaccc cgtagccgca
    11881 gccggcgtaa tgtgtcgcaa tacggtttcg ataggttggg atggctttat ttatgattgt
    11941 gatttcaatc agatgctcgg attacatgtg aattcaggtg caccaaatca tatttcgaaa
    12001 tgggatactt acgcattaca atcgcgcaac attgtattga accaacattg ttacggctgt
    12061 actgcaggca gtggaagttc atgcggtggg gctgtagcgt aatcaacaaa ctcaaacaat
    12121 agcaatgact caagacgcgc tcataatcat gattaaaaat ccggtcaaag ggaaggtaaa
    12181 aacccgcctg gctgccgaca tcggtgatga tgccgcttta gctatttata tgcaattgct
    12241 ggaacacacc aaacaggtaa cagaaatgat tccgcaggat aaatttcttt tttatgatca
    12301 tgtagtggaa cgcaacgaca tttttcctaa cagcacatac aaaaaatata cccaatgcaa
    12361 tggcgacctg ggcgtgcgta tggattacgc tttcagcatt ccattcaaaa atgaatataa
    12421 aaaagtagta atgattggct ccgactgcta tgcacttaca gcagaacatc ttgcaaaggc
    12481 atttgaagca ctcgatcatc acgattttgt gattggtccg gcacatgacg gcggatatta
    12541 tctcataggt atgaaaaaat ggaaccgttg ggttttggaa aataaaagct ggagtgagca
    12601 taaattattt caggaaacgc aagctgacat cctcagcaga aatggcacac ttcatctatt
    12661 agaaccacta agcgatatcg atgaattaaa ggacatacaa gctataagtt tatttaaatc
    12721 actgatacag gcatgatcgc aattattatt ttactggcag cactatttct cgcctattca
    12781 aatggcgcga acgacaactt caaaggtgta tctacactct ttggcagcgg caccacaaat
    12841 tttaagaccg ctcttcgatg gggcaccatc acaacactag caggttgttt atttgcgatg
    12901 tatatcgctc aagggttaat gaaaaatttc agtggaaagg gattgattcc tgatgacacc
    12961 ttacaagacc ctaacctcgc aatagccgtg gccattggcg cagcagccac cgtatttcta
    13021 gcaaccagga taggcatccc tgttagtacg acacatgcca ttgttggtgc acttaccggt
    13081 gcaggattta ttgcggtagg aagcgaattg aatgttaatc aacttggtga aacattttta
    13141 aaaccactca tcctcagccc atttatagca tgtggtgcca gcattatatt atatctgcta
    13201 ttccgtcaaa tcagaacctg gttgcgcata gaaaaagata ccacgcttga aatacaggaa
    13261 ccgcactttg cacaaaatag cataagcgca tcaacaattt ccctcactca gcaacctaaa
    13321 gaatcttata ccggtcatat cgttggtatc agtgcacaaa aaatattgga tagcctacat
    13381 tttatcagcg ccggtgcagt aggcttcgcc agaggcatga atgatgcacc gaaaattgcc
    13441 gggctattat tgctcgtgca ttttctaaac gtgccttcca tgatcatcat tgttgccgca
    13501 gtgatggcag ccggtggatt attaaatgcg aaaaaagttg ctgaaacaat gagtcgcaaa
    13561 attacataca tgaatcccgg tcaaggattt acctcaaatc tggttacagc cgtattagtg
    13621 tggacagcaa gcatgagcgg attaccggtt tcaacaactc acgtttccgt aggtggtatt
    13681 ttcggtatcg gagtagtaaa tagaaaagct gattacaaaa tgataggaaa aattgttggg
    13741 tcgtgggtac ttacacttcc aattgcagca gttttagcag ctacaacatt ttatatttta
    13801 caacaattca attgaaaaat taaataaaac aacaagcatg tcgacaacgt atttagaaac
    13861 aacacgcaat gtgtacaaag aagcagctga aaatccgcag aaaggattat gctgcaccac
    13921 cacgcctgtt tggcaattgc ccggattggt gattccacaa aaaatgctgg agatgaatta
    13981 tggttgcgga agcacggtta accctcgcga tttggtaaat gatcctacca ttctatatgt
    14041 cggcgttggt ggtggcatgg aattattaca attcgcttat ttctctcgtc gcaaaggtgg
    14101 cgttataggt gtagatgttg tagaggaaat gttgcaagct tgccacgaaa atttgcttga
    14161 agcagaaaaa ttaaatccat ggtttaaacg cgagtttgtc gacttgcgca aaggcgatgc
    14221 atttaattta cctgtagaag ataattctat tgacatcgct gcacaaaatt gcttgttcaa
    14281 catatttcgc gatgaagacc ttgaaaaagc gctgaaggaa atgtatcgca ccatgaaacg
    14341 ccatgcgcgc ttggtgatga gtgatcctac atgcgaacag gaaatccctg aaaacttgcg
    14401 caacgatgaa gaattgcggg cactctgcct gagcggttca ttgcctttgc aagaatatat
    14461 taatcgcatc acaaatatcg gtttcggaac cattgaagtg cgcgcccgtc gcccatatcg
    14521 cattttggat ccaaaacatt atgctaccaa cgagttactt tatatagaaa gtgccgaagt
    14581 gtgcgcaatt aaagatccta tgcctgccga cggaccttgt gtattcaccg gcaaaacagc
    14641 aatctatttc ggcgatgaag aatatttcga cgatcacaaa ggacatgtga tgacaccaaa
    14701 ccaaccactt gccgtttgcg ataaaacagc aggtaacctg gctgcactga atcgcgatga
    14761 tatttttatc tcaggatcta catggtttta cgatggcggc ggatgctgct gatcgatacc
    14821 cgctttttat cagccccaac ccgacagttg gggctttttt gtggtccaag cctcgtatct
    14881 ttatgcctac ctcaatctaa aggcatggaa aacaacaact ggagcaataa ttccgttttc
    14941 aaaacaaccc tcatcgtcat tcttacattg gcaggattgt ttggcgttgt ttggttgttg
    15001 atgcagatca cttcaattct gttttatttt ataattgccg caatcatttc tattatcgga
    15061 aaacctattg ccgacttgct cgacaaattg catatcggca aattcaaaat gccatcttcc
    15121 ttgtcggcct tgttaactat cggtgtttta tatggtatcc tctttggcat catcagcata
    15181 tttacaccac tggttattca acaaacaaat accatcagcc atatcgatac ccagaaagtg
    15241 gcagacggac ttcaacagca agtacaagtg ctcgactcta tgctcgtgca tgtacaggac
    15301 cccactcaac ctgctaaatc cgttgaacta atattaaacg aatcattgcg tgaagcaatt
    15361 aatgcaacat cgctcacaca tattttcaat aatattttag gcggactcgg aaacgtgatg
    15421 gtggcgctgt tctcaatcac ttttattacc ttcttttttc tgcgtggtga acgattattt
    15481 tataatatca tcatggtact cacaccaacc aagcttgaag gaaacgggcg gcgcattctg
    15541 cattccagca aaaaatatct tacgcgctat tttgtcggtt taataattca atcgacagtc
    15601 gttttcatcc ttatcgcagt tggattgttg ctattgggat ttggcgccaa tagctggttg
    15661 attgcactct ttgcgggtat tattaatatc attccataca tcggtcccat catcggaatg
    15721 ttgtttggta tgctgattgg tataaccact aatctgagtg ttgatccgaa tttcgatatc
    15781 attccgatgc tgatacaact actgatttta tttcagggtg ttcagctcat tgataatttc
    15841 gtatcgcaac ctatcatcta ttccaacagt attaaagcac atccgctcga agtgttcttc
    15901 gttgtactgg tgtttggctc cctggccggt gttcccggaa tgattgcagc catgccggta
    15961 tacgcctttt tccgcattat tgccaaagag tttttcagcg aatggaaggt agtgcgcaaa
    16021 atgacgcaag acatataaat gttgcagatt ttatttcgag atgtgacaag gtaataacct
    16081 cgagatcttt tataacttat tgataattaa tcagtcgtac acagaaaagt gcacattttg
    16141 tgacatccgt gtataaacag ccattttgaa atcaaagaaa aggagttgtc tttatttcac
    16201 cctaccgagc gacaagcgca caccccactt ttaaaactca ttttatgcaa aagacaattc
    16261 tgtatgctac agctatagcc ttgtgcggct ggctcattaa cacatcatca attattgccc
    16321 aaacagcagt tcgtcccgta gcccaaatgg tacgcgatgc ccacgcagca aagatcaatt
    16381 ttacccctgc tcaaaccctg gatcgggtat ctgctccggc atatcgcagc aaagctgagg
    16441 ctttcgccaa caacattact tacgcgcgac ttgatggtga tgcaatacat cagttgtttg
    16501 aagaaaaaca agcggcgatt tcattgacag ttccttataa agaaggtcaa aacctgacct
    16561 tagaacttgt acaagtaaat ttatttgccg acggatttaa agtaacaacc agcgaaagca
    16621 atggtgctgc ggtagaatat acgcccggtg tatattatca ggggattgtg cagggcgatg
    16681 ataattctat tgccgctatc agcatttttg aaaatgagat gatcggcatg gtatccactt
    16741 cctccgaagg aaatatcaat atcggaaaat atggcgatgg cgactggcaa gattatttgg
    16801 tgttcagcga cagagatatt attgttcaac ctgaaaatgc aggatgtgca actgtggatg
    16861 atgaatcgta tctcgacacc tttcatcaga ttatggaaaa tggcggtggc gaacgtatta
    16921 ccaaatgccc gaaaatttat tttgaatgcg attatcagtt gtatcaaaat aaagggagtg
    16981 ttaccaatac cactaattat attaccggtg tttacaataa tgcagctatc atttatacca
    17041 acgaaactgt tccgacacag atatctgaaa tttttgtgtg gacatcggct gatggttatg
    17101 ctacagcttc atcatatgat gcgttgaatt catttaaagc ctttcgaaca acttttaccg
    17161 gcgatattgc tcaccttgta acgcttgatc ccggcggact tggaggcatt gctgcaacga
    17221 tcaacggatt atgcaattca aataaatatt gctacagcga tattgatgca acctattctg
    17281 catttcctac ttacagttgg acagttatgg tggtgacaca tgaaatggga catttattcg
    17341 gaagttatca tacacataat tgctctgcat gggtaggtgg tgctttagat aattgttaca
    17401 caactgaagg tggttgccct cccggcccgg caccaaccag tggtggaacg ataatgagtt
    17461 attgccatct tacaggatat ggcattaatt tgagtaatgg cttcggcact cagcccggaa
    17521 atgcaatcag aaatacaatt actgctgcag catgtatcac atcatgtggt ggggttccaa
    17581 cttattgcac cagcgttgga agctcaactg ccgacgaatg gatacaaaca atttcaatgg
    17641 caggtacaac taacaactca gggaacaaca gcggatatgc cgatttcact gcaacaaaca
    17701 ttaaccttac acccggagga actgcatcat tcacactgac tcccggttac accggtacag
    17761 tttatcctga atatttttct atttggatag attataataa agaccttgat tttgcagatg
    17821 ccggtgagaa tgtatataat tcaggtgctg ttacttccgc agtttcagga agctttagtg
    17881 ttgccgcagg aatgacaggc acaacacgca tgcgcatcag tatgaagtat aatgcacttg
    17941 ccggcccatg tgaagtattt tcttatggcg aagtggaaga ttatacagca tcatttgttc
    18001 ctgtagttac ctattgcacc agcgcaggta caagcgcagc atcacgctat attgattacg
    18061 taaaaattgg cacaataaca cgcgcatcag gaaatgatgg cggatattac aacggcactg
    18121 caatgagtgc aaacgttatt aaaggatcaa gctatacact tacttacagc gccggcttca
    18181 gcggtactat ttatcaaatg tattggagag gatggattga ttttaatcgc gatggcgatt
    18241 tcgcagatgc aggtgaacaa gtattccaaa aaaaggctac cagtgccggc aacttaaata
    18301 aaacaattac cattcctact acggcaggaa caggtactac acgcatgcgc ataagctgca
    18361 aatacggcgg ttatccaacg agttgcgaaa catttgccaa tggcgaagtg gaagattaca
    18421 ccttgaatat tatgccggga ttgccttctc ctgaagaaaa tccttattac ctgaatatat
    18481 acccgaaccc atcagaggga gtattccaaa tggagttcct caatgcattt aatggtggca
    18541 tcactatgga agtatttgat atgacaggta atcgcatacg tcaggaagtg atcgaaagcg
    18601 ataatggctg gatagtactg gatatctccg atgccgcacc cggtatgtat atcgtaaaaa
    18661 ctacgcttgc aaacggaaca accgaaagca aacaaataat taaacagtaa aacccaatgc
    18721 aatatttacc gcctttcgta tgttgccaac atatgaaagg cggatttttt ttgaaccata
    18781 taacttgtct gagcgtatat gttgatatta tgaagcacaa caaccttatt tatcgcaccc
    18841 tcatcgggat gatgcttgct gtagtattat taccggcatg cagaacgata gatggtatcg
    18901 acagcaacag caaaccggtt tcacatgaac aatggtcggc attattggac aaacatgtcg
    18961 atgataatgg atttgtcgat tataaaggct ttatcctcga cagcactgca ttaaacgact
    19021 atctgcaatt attggaaaaa ggttatccca acgaaaaaaa ctggacacgc gaggaaatcc
    19081 tggcttactg gataaatgca tataatgcct atacagttca attgatcatt cgaaactatc
    19141 caattgaaag catcaaagac attaagcccg gagtagcatt tatcaattcc gtatgggatg
    19201 ttcaattcat taacatagaa ggacaggaac ttgatttgaa taatatcgaa cacaatattc
    19261 tacgcaaaat ggaagaacca cgcatacatt ttgcaattaa ttgtgcaagt tattcatgtc
    19321 ctaagctccg aaatagtgct tatacagccg aaaatctaaa tacgcaactc gacctggccg
    19381 cacgcgattt catcaacgat ccatcgcgca acaacatcaa aaacggatca gcacaactga
    19441 gcagtatttt caactggttc accggcgatt tcacgaaaaa tggctcattg aaggatttca
    19501 tcaatcaata cagcaacaat aaaatagccg ccgacacacc gattgaattc atggaataca
    19561 actggaaatt aaacgagcaa aacggcggaa aataatgtct tttcctgtct ggcaatccag
    19621 gtttaacaaa attttatact tcatacgcga actattccgc atttgttcct attttcacca
    19681 tgcgtcttta acaggcaacc tctctactca tgcatcatgt aataataggt aatggtatta
    19741 ccggtatcac agcggcaaga acgctgcgaa aactgagtaa ggatcatatc acggtcatct
    19801 caggtgaaac agatcttttt ttcagccgca cagcactcat gtatatatac atgggacata
    19861 tgcgattcaa ggatacacaa ccatatccta aagacttttt tgagaaaaat aatatttccc
    19921 taaagaaagc ctgggtaagt aaaatagata gtgcaaaaaa aacacttcat ttcgacagcg
    19981 gtgatcatat gcagtatgat aacttattga ttgcaacagg ctcgaaatcc aatcgcttcg
    20041 ggtggccggg tcaagacctt aaaggggtgc aggggctata cagttatcag gatctgttag
    20101 aattagagga aaatacaaga aatggtatta accgcgcagt gatcgttggt ggtggattga
    20161 taggaattga gatggcagag atgttacatg caaggcatta tcccgtcacc cttcttgttc
    20221 gagagtccaa ttattggagt aatattctgc cggcacaaga atctgctttg attaccaacc
    20281 atatacagga tcatggtttc gatctgaggt taaataccac cttaaaagaa attaaaggtg
    20341 atgcaaacgg tcgcgtaacc ggaataatta ctgattcggg agaagaaatt ccttgtcagg
    20401 tagttggcct cacggccggt gtttcaccaa atatcagtgt tttacaagga acagaaatta
    20461 aaacagatcg aggcgtactg atcaataaac gcatgcaaac ctccgtagaa ggtgtttttg
    20521 cagccggcga ttgcgcacag tttacggaga ctctccctga tcgtaggcct gttgagcaag
    20581 tatggtacac cggaaaaatt caaggcgaat gtgccgcaaa aaatatgttg ggaataaatg
    20641 ctgagtatga tcccggctac tggttcaaca gcgcaaaatt tcttgacatt gaatatcaaa
    20701 catacggcac ggtacttagt aagttacgcg acggcgaatc ggaattttat tgggaacata
    20761 acgatcgaca aaaatgtgta catatcgtat tcaatagcag cgatcataag tttatcggca
    20821 ttaatacatt tggtatcaga atgcgacata aactttttaa tacatggttg caagaatcgc
    20881 gaagtgtaga atatgtgatg gcgcatttgc gtgctgcaca atttgatccg gaattttatt
    20941 cccgctacga agaaaatatt attgatgcct ggcatgcaca attccccgaa catcgcatta
    21001 cacagcctga tgaaaaaaca attcgatcac tcattttcaa cgcataaaca ccatggctga
    21061 tcaacaaata gacatgtcgc tgtcaggcag ctcacccgcg aatttcaatg gtttacaaaa
    21121 aaccggaatc gccattactt caatcggtat cttattcttt tttatagcct ggttcggtgt
    21181 cggaaaccaa caaccactga tctgcctgtt aattacattg gcaggattta ttattggtgg
    21241 cattacctat agttttggta catatggaca tctcccaaag ggcataaaaa ataatcgcgc
    21301 attctttaca tcaatctcat cacgtggaac gcttggatgg atttccggaa tcgtgattac
    21361 tgcattttat attgcattat actggcgcga ctactggaaa tctgttttac ataccgatgt
    21421 ttttgccggt ctggtaagca tgttcgatcc cctgagtcaa tggatgcgga aaggacctgc
    21481 ggatcaatgg tttgtttatg gttctttata tacgctcgct gtttttgtga tgggcattaa
    21541 attcatactg aaataccgac acaacagata tcaggtaatt cgcaccatat cggtaatgtt
    21601 ttttcaattt ggatttgcat ggattatacc tgccgtaatg aaagcgctct ataatttcga
    21661 accttattac agttatttct ggccacttga ttatgatgct ttctttccaa attctataaa
    21721 ttatttcaaa gaccagggaa acttgggtgt tttcttttta ttctggggag cagtaatggc
    21781 attcatcgcc acacctatac tcacttactt ttttggcaag cgatggtatt gcagttgggt
    21841 atgcggatgc ggtggattgg cagaaactgc aggagacccc ttccgccatc tttcagataa
    21901 gagcctgaaa gcctggaaaa ttgaacgtat caccatctac tctgtgctcg gagctatcac
    21961 tttgattacg gcagctttgc tcatagataa ttctatgcac aatcaattgc tgggttcttc
    22021 ggcaaaatgg atagccaagg gatacagttt cgccataggc atggtattca gtggggtgat
    22081 cggcgtgggt ttctatccgc ttatgggtag cagggtttgg tgccgttttg gttgcccgat
    22141 ggcagcttac atgggtatca tccaacgctt caaatcgcgc ttccgcatca ctacaaacgg
    22201 cgcccagtgc atatcctgcg gaaattgcag cacttattgc gagatgggta tcgacgttcg
    22261 tgcttacgcc cagcgtggtc aaaatattgt gagagcaagc tgtgtcggct gcggcgtttg
    22321 tgctgccgta tgcccaagag gtgtcctaaa actggaaacg ggccccgaaa atccaagccg
    22381 gactgaggtg ttattcacat ccgacgactt gaaaattctc tcctgacata ttaggtttat
    22441 actgtttaaa agcggtaact ttgtgcactt tttcagagaa gggcacggga acggaaaagc
    22501 catggcaaag atcaatgtaa tcatcccggt ttttaacgaa agcgggtcta ttggtcacgt
    22561 gttaaatgat attcccgccg gactggtcga agaggtaatc gtagtgaaca atggttcaac
    22621 cgataatacc gcagaaatcg cccggagcaa aggtgccaca gtgctgttag aagcgcggag
    22681 gggatatggt tctgcctgtt tgaagggcat agcccatatc aggagaaaac cggtagagca
    22741 acaaccggat atagtagtgt tcattgatgg agattattcc gactatccgg agcaaatgcc
    22801 tgacctgatc aggccgatta cagaacaagg gttcgaaatg gtgatcggca gcagggaaaa
    22861 aggcactcgc attaaaggaa gcatggcccc acagcagatc ttcggcaatt ggcttgccac
    22921 aagcatgatc ctctggttgt ttggagcaag attcagcgac ctgggacctt tcagggctat
    22981 tacctggaaa gcacttgaat cgcttgaaat gcgcgacacg aattatggat ggacggtaga
    23041 aatgcaggtg aaggcagcca aaaagaaact gcgctttacc gaagtgccgg tgaactatcg
    23101 cccaagaatt ggagtttcga aggtcacagg tacagtgaaa ggaaccatca tggccggata
    23161 caagatcatt acaacaattt taaaatatta ctaacctaac ctctctacat gtcgatcacc
    23221 atcatcgtca tttacaccat tgccatcaca ctccttttct tctactgtat catgcagttg
    23281 agcctggcta ttcactacat ccgctataag cgccgtatgg aagccgaaga agcaaccttc
    23341 aaaggtcctg aatcggaata cccaatggtt actgttcaac ttcctgttta caacgaactc
    23401 tatgttgttg aacgccttat cgacgcagtt gccgctttcg attatccaaa agataaactc
    23461 gaaatccaac tactcgacga ctctacagac gaaagctttg agatcgcccg taaaaaggta
    23521 gatttctata agcaacaagg cataaacatt gttcaggtta aacgcccaat ccgcaaagga
    23581 tttaaagccg gcgccctgga atatggtttg caaactgcaa aaggcgaata cgtggccatt
    23641 tttgatgccg actttgtgcc atatcccgac ttcctgaaaa aaaccatccc gtatttcctt
    23701 accgatgaaa agatcggtgt agtacaagcg cgttgggaac atattaacaa ggattattct
    23761 atcctcacta aaatgcaggc atttgcattg gatatgcatt tcaccatcga acaacaaggt
    23821 cgcaatgccg ccggttattt catcaatttc aatggtactg ccggtatatg gcgtcgaact
    23881 gctatcgatg atgcaggtgg atggaacagt gataccttaa ctgaagatct ggatcttagc
    23941 taccgcgctc aattaaaagg ctggaaattc aaatatgtgg aagacgtagt tgctcccgca
    24001 gaattaccta ccgatatgaa tgcactgaaa tcacagcaat tccgctggaa caaaggtggt
    24061 gcagaaacag cgcgcaaaat tggtcgccgt gttatgggtg ccgaattacc tatacgaatt
    24121 aaattgcatg caatcgcgca cttatttaat acaacgaatt acatttttat tttgattaca
    24181 gcaatattga gcgtgccact gctgtttatt aaaaatcatt atatcgactt taattatttt
    24241 aaatacgcct ctattttctt aatgggctct atcgccatcg catatgctta ttatatcagc
    24301 accctgcaac gcgagaaaac tgtaggacaa accgtgaaag tatatatttc acgcttccct
    24361 gttttccttg ccatcacaat gggtatgagc ctgcataatg catgggcagt ttttcgcgga
    24421 tggttaggtc aacaaacagc atttgttcgc acacctaaat ttgcaatcaa caactcaggc
    24481 gatatctgga agaaaaagaa atatgtagca tcgcgtgtaa gtcctattac ctatcttgaa
    24541 ggtatattca gtttgtattt cctttctgga attatcatgg gtatctatta tcaggattat
    24601 ggactgttgc cattccatat catggcattc atgggatttg cgttgatctt ctatttctct
    24661 ttcaaacacg caaggtctaa ctaaaaaata actaatggaa aaccgtacaa ctcgtacatg
    24721 gcaaatagcc gcctacgtgt tgtacggttt tttgtgtacg atattattgt atttcacctc
    24781 acgagaaaat tttatacagt taattgcgat ttacgccggc atgtttgcac tgtatttctt
    24841 gctcgcacga agcagtgaaa gaacaaatta tcttggtgaa ataattatcg ccggtatcct
    24901 gcttcgattg attgccacct ttgcattacc aaatctcacc gacgatcatt ttagatttct
    24961 ttgggatggt caacttgtaa cacatggctt caacccttat gcatcaattc ctgaacaagt
    25021 gcgtgctgaa ggctattttg gtatttccac cttggatgat catttattta atagtttaaa
    25081 ttcagcccaa tatatcagta tttaccctcc aattgcgcaa gcaatctatg ccattggtgc
    25141 actcattggt ggcagtaatt gggatataca gattctggta atcaaaataa tactacttgc
    25201 ttttgaatgc ggaaccatat tcctattgcc aaaagtgctt aaacaattac atatcaatgt
    25261 cggacatgcc gcctggtata tcttaaaccc cttgattatt gttgagcttt caggaaatat
    25321 gcatcttgaa agtacaatgg ttttcttctc attattgtca atatattatt ttaacaaaaa
    25381 caatctttgg ctatcggcaa tttcgtttgg acttgccatt gcaacaaagc tatggccttt
    25441 gatgttgatg ccgttgttat ttcgtcaaat cggttttaaa caaaccatgc gttattcagt
    25501 tatcgcagga gttacggcaa ttatttttct attacccatg ttgatagagt tctcaaaaat
    25561 caccggcagc ttcaatctct attttcagca attcgaattc aatgcatcca tattttatat
    25621 cggcaaatat ttcttcgata aggatgtcaa ttacgaagct ttcagcaata tgcggggaag
    25681 cctacccttt attgcagcat ttttgatttt ggtgttcagt gcggtttaca aaaaacagta
    25741 ttggtatgcg ggtatgctga tagccttttc tatttattta cttcttgcaa caaccgttca
    25801 tccatggtac ctggcaccat tgatattgct atcggtatta agcggactga ggtatcctat
    25861 tttatggtct ctgctaatct atttatccta ctacacctat atcactcctg cctacattga
    25921 aaacatgacc tttgtttgga tagaatatgt aatggtgatc ggatttgcgg cttatgaatt
    25981 aattcattcc cccatgattg aatccaaaat aattacctct cgcctacctt tcaaaatata
    26041 attcaacgcg acggtacaat tggtgttcta ccacactttt ctcatttttg atatggtctg
    26101 aagtttcacc tttataaaca actatcaggc ggctttcagg aacacctttt gaatgcaggt
    26161 agttgacgac ttcgccggca cgatctgcag ataattttac gttgactcct tcatcggatg
    26221 tggaatcgct gtaaccaaca acacgtaagg ttaatttatc gtagcgtatt aaaatgtcgg
    26281 caatttgatt tagagaagga acagcatcgg ggcggataac agcttctgcc ggatcaaaaa
    26341 aaatataggg taaacaccag gtagtgtatg catgctccac atattctcta cctgatactc
    26401 caacatccca aatgcagcct atgcttacat tcacacaatt ctccataggc aatacagggc
    26461 ttgaataagg ttcttcatct aagtggtcag gacaaccatc gtggtctgag tctaatgtta
    26521 ctccgtgtat atcaactgcg gcccaatcat ctgaattggg ctccaagtcg attgtattat
    26581 atacgccatc attatcatca tccaacaaga gtgaatccgg atcaaaatac acctggcaaa
    26641 taatataatt aagtggaaaa aacgagaaaa taacttgatt ttcgttaatg tcaacagtaa
    26701 cccgatagga attaaatttg actgaatccg ataatgcgtt ttttgcataa cacagatgcg
    26761 taaggcaaat taatatccat aaaagagtga gtttcatgcg ctatagattg caggttagct
    26821 tatatagata catcttagaa tcacttcctt aggtgtttag ttgctttcaa tccaaaactc
    26881 aactatacga ggaaaacctt ctacaatatc accgtcgtaa aatatctgtt ttggctttct
    26941 atattttact acggaaatcc tgtcttccgg aacaccaagt tcaatgagat aattaacaga
    27001 actaattatg cgcctttcaa ccagcagtgc tgaggaatct ttctcgacgc caccagctga
    27061 aaatccatac gccctgatac tcagcatcgg ataccttatc attatttcag caatcattgt
    27121 taaggccgct tccgcatcag gtctgatatc agctttatgt gcgtcaaaaa atatgaaggg
    27181 aagtatccaa tcatctgcac ctgacaattg attatctagt cgcaggtgag cacaactttc
    27241 tattgtgagc ggaatttcct tataaacatt atggcaatca accattgaat caaatacatc
    27301 agaatacggc tcggggtctt ccgcatcaat acaaccatct ctatcgctat cgaggctcgc
    27361 accgtgaatg tctacaggag ataaagctgg cgtattcggt tccttatcaa agcgattaac
    27421 aacgccatcc atatcgctat ccatcgtatc caattcagaa gtatatgccg gaaaatcgta
    27481 taaatacgaa agcggattag gataataaac acaaataact tgccggtgaa acaatcgccg
    27541 atgcttgtgt gctttcgact cattatcgtt attctgagca tgtaccggat ttagcagata
    27601 cacaacaaaa acaagcgcca atattttaca tcgtagcaac aaaaatggag aaattgtaat
    27661 atgcaattta atgcatttta agcttaatta gaaatatcag tttatataaa taaaaaaaag
    27721 gggtgcattg ctgcacccct ttggctgtca tgattatata tatgaatcag tttttaataa
    27781 tgtggaactc aacgcgacgg ttcatttggt gttctgcttc ttcgttcgcg tctttgataa
    27841 ggttttcagt ttcgcctttg taaactattt tgaggcggct ttcaggaaca cctttgcttg
    27901 tcaggtagtt gatggcgtta cgagcgcggt cttcagacaa tttcatgttg aattcttcgc
    27961 tggcacgagt atcagtgtga cctacaactt ctacgctcaa tttgtcgtag cgtttcatca
    28021 ggtcagcaac ctggttaaga gcaggaacag cgtcagggcg gatgttagat ttgccaagat
    28081 cgaagaagat ataaggcagg atccagtctg tgcttgctcc aggagtgtat ttagaaaggc
    28141 gctgatcaac atagctttct acccattctt gagtcaggtg aacgttctga caattttcca
    28201 taggaagaac aggtgaagag aatggttcag ggtcttctga gtcagggcag ccgtctttat
    28261 cgctatcgag gctaacaccg tgagtatcaa caggagttcc ttcaggagta tttggttctt
    28321 tgtcgagacg atcgataaca ccatcgttgt cagtatcttt caacatgttg tccggatcga
    28381 tctcagccat ttgatcatac agaacagcaa gtgggttaac catccacata ggatcgatag
    28441 actcagcttt accaaggtta taaccaaagt tcaaagaagt ataagttaag aggtcgtagt
    28501 gtggggaata tgtaccatca tactgccaac gctgaccgtc agcgaggtca tcgaagtagt
    28561 gagtagctct ttgatcgaga cccaattcca ttttagaacc gatgcgcata gacacaccga
    28621 aaccgccgga gattgaaggg tttcgcaccc aagtagcacg aacttcatca gcaccacgag
    28681 cagcagggtt cgctacctcg gcttgtgttt catactcacc atcaagggca gcatgtaacg
    28741 cgtcgagacg ttctttctga gaggtgttct gagatccgaa accttcgagg atggtttgga
    28801 atggataagg attaccatca gcatcaagtg catcatattt ggtattgtaa gccatagcac
    28861 caacgccggc atatgcataa gcatagaacc taggatcgtg ctggtggaag ttcaggttgt
    28921 tcagattcac aatgaaatcc aatgaaccat gaccaaattg agtcttgtag ttaggtaccc
    28981 aaacgttgtt ggtgtagttg gcacctgtag agtaatagtt ctctgccggt tggccgtcaa
    29041 cattgatcgc atcgtaaaat gcaccgttca atgttttgtt gtaagcataa gatccctgaa
    29101 cgtagtccat tccataggtc tgtgcataca ttccatgcaa acgcaatgaa acgatgtagc
    29161 caaggctttt acgaacgttc aaaccaacac caaatgcagg agcgtggtta ccgccgaaag
    29221 gttcagctct caaatcgccc tgaatgatag ggagaccgag gttaagacct atttgccatt
    29281 ggtcggttgg tttaggggga aaggaagatg tctgtgcgaa agtaagaccg ctatagagca
    29341 gtactatcgc aatcgtgagt tttctactaa tcatgacgta ttaattaagg tggttaaaag
    29401 ctaattcagt taaataaaaa atgtcatata ataatcgaga gcaaagctaa gaggcatgta
    29461 cctcatatgc aagtacttat gcaggttttg tggaattttt ccccattttc cgtgcatttt
    29521 acatgcaaat taccggaatt ccggtaaagt gtgcggaggg ggtagatcac actaaaagcc
    29581 ttatggcata agcactttta agtcctcagt aacaaagcta gccacttcga ccaaaaaacc
    29641 aaaaaaaaaa tgaaaaaaaa ttatccggtc gtgaaccaac ccaggcatac cagcgtcaag
    29701 aactccttaa cggagcaaag tgatggttcc ggacttagta atcggggtgt ttagggtagt
    29761 agtagacttg atcacataca cataggtgcc tatttcctgc ggaagcccac ccagcttgcc
    29821 atcccagccg aaattgatat cggtggaatg aaacacttca gccccccagc ggtcgtatat
    29881 ccgaatgtca taactgatca ataggtcggt tgatatatta aatacatcgt ttaaaccgtc
    29941 atcattcgga gaaaaggcat ttggcaccac aaaatctata tccacccaaa tatttacgga
    30001 atcgctagcc tgacaaccat tcgcatcggt taccgttagc acataggttg aattgaaagt
    30061 tggcgtgcat gtaggctgaa ggcaatcaat gcacgacaaa taatctaccg gcgaccaact
    30121 gatggctgtt actacgggag ctccggttgt tccaagcaat accactgatt gaccatatgc
    30181 aatgctctgg tcgggtcctg catcaaccat tatttgtggc ggctgactga ttacaactcc
    30241 ggtaaacgac gttgtgcatc caaaggcgtc tctcaccgta aacgtatacg tgcctgcttc
    30301 gaggctgata aaattgctgt cggtttgcca gttaatacca tcgaaactaa actgatacgg
    30361 caatacgccc cctccgggat tgaggttcac aacaccatca gatccaccga aacaggttac
    30421 gtctacagtt agaacggtat ccaacgtgag taatggaggt tcggtaatta caacatcttc
    30481 ctcccaggca caaccatcct cttcctgtat tgtaatagta taggttccgg caccaagatc
    30541 atatatactg tcaattgttg agccggttga ccaggtgtaa gtaattggat ttgtgttgcc
    30601 ggttacatca acatcaatcc aggcatttgt tgcaccaaag caagcaattt gctgcacatc
    30661 cagtgtaacc tcatatgggg tgctgacagt tacggtaacc tcatcggttg ctatcgtgcc
    30721 atcgcacagt gtaatttctg ccgtgtaagt tgttgttaca tcgggattaa cataaatact
    30781 atcacctgtt ccaacaacaa taccggtggc ggtttcatac caggtaatgg catcggggat
    30841 ccagcgccag ctttcatcat cggcaaacca gtctgttgca tttctgcctg ctactgaaaa
    30901 ggcttctgtg ccatcttcat ttattatacc ctgcacggca acgcctaaat cccaggtagg
    30961 acaaacatct acatgcgtca ggtgattttc aatcacattt gtggtttcat gcaatacaaa
    31021 ctgaaatgtt ccttcaaagg cagtgcatga gaaaagcggc acatcttcat atgtaacaat
    31081 aagcttgcga tttggggcaa caccaactac ctcatgaaaa atacaattcg tgcaaagccc
    31141 cgaatgccag tcggtccacg gtccgaatat ggcaggtttc ggagttactc ccgcaccaaa
    31201 aggaataggg ccatcaggag tccagttcac atccattcct cccgttggtg taagaaaact
    31261 aatccaaccg tttgaggcaa tataaaactg cgtatacact tgtccgaaaa aacaaaaatc
    31321 gaagccaatt gaaaaagggc ctgtatgggt atcatccacc atattatgca cggttccgcc
    31381 gataggctcg ggggcgtaag ggatttcggc ataggtatat gattctgtac cgtatgatcc
    31441 atcaacaact gcatataaca tcaccgaatc ggcgctacaa atagttgtat ccggataggc
    31501 agttatactc tgggaatatc ccgttgatag tatgcccgcc aataggagga ggagcgtgca
    31561 ttttttcata ttgattacac ttgtgaccgg ctaataggct ataaatataa acataataat
    31621 ctcagctgcc ggctttattc agaaattgca ccaagggatg tgcagatttt agtgtattca
    31681 ctacttcttt cagaaatgaa gggtctgtta cttctttgtc gctaaacgcc ctcgaaacca
    31741 cataactctt atgccgtaaa tagggaagtg cctgattgtc ggggctgtag cccttaggcg
    31801 tagttttgag cacttctatt tcatcgagac cttcatagta ttttttgaat gatgcggcct
    31861 ttagtatttt ttcaaaatcc ttgaggttgt agtcgatttc ctgacgcatg gcagccaaca
    31921 ccggaagttc aggcatatag gccccggcag cggcaaagga ggcatttccc ggttgtattt
    31981 gcaggtagta gcccgctgaa agcccggatt taggccccat acccaacgat gcgccgaaat
    32041 tggttttata ggggtttttg tcttttgaga agcgaacatc ccgataaatg cgaaaaacag
    32101 cctttttggc atctataccc gccattgcag gctcgaagga ggctgttttc tccaaaaccg
    32161 cggcggtgaa atcgataaaa ttacccttgg cagcctcgta aatggttcgg ttgtcttcga
    32221 accatacgcg atcgttatgg gcggcaaggt cttttaaaaa tttaagtgta cttttttgaa
    32281 tttgtgacat aaaacttata tttgcatact caaaatatca gcttctgctc atatttcagg
    32341 caaaatctaa gattaaaggt ataattaatc ctcatcatga actgcaaacg ggtaatatta
    32401 aaagctgtat tttcggttat taccattagt tcatccatgc tacgtaccgg tatggcacaa
    32461 gatgagtttc atgccgaagg ggtgaaaaca tataattacg gaactttcaa tactgcagta
    32521 tccgatacac ctaaagaatt attttcactt acaccagatt cactttatac ccatccggag
    32581 tttggcatat taccatataa tgcaccatgt gaagattgca ttgaattatt gcaataccga
    32641 actcagaatt cgcgcatgtt tgtgaaaaag ggaaccaatg gaagtaaatt ttattcgcaa
    32701 ggtgcatatc aggatttaaa ttacaaggat aataaaggaa actgggttac tatagatcca
    32761 acgcttcaac catctactgc attacccggg gtttatttgg gattacatca aacaaatcct
    32821 accacattaa atatggtaga aggatatact gccatagcca tgcaagacgg tgcagaatta
    32881 agatttaatc aaaatatcag cgcatatgca acgtctgaaa ttggtttaaa tattccgggt
    32941 attgccatca accgatctgt ttattctgtt ggtgctgatg gcgccgccgt taaagatgct
    33001 tttccaggaa ttgatcagga attgcgattt aatgttgcag aaataaaatc aggatatact
    33061 gtttggaatt tgaatgcgat tgatacatca aaaaaatatt ttgtcattcg cgatgaattt
    33121 gatcttcctg aaggatacac catcagcaca gatcaatatg aaggtacatt taatgtaaac
    33181 ggattctggt ttggcgatct ggttatagaa aatgccgatg gatttgaaat ggcgagaatg
    33241 aaagctccgg tgatgtacga cagcaatccg aatgacactc ttgactttgt tatccctatg
    33301 gatattattg gttacaccgt gaaacaaaat ggcagaaccg taaccttaaa attggttgcc
    33361 gacatggcgt acctgcgcga tccgcaacgt caatttccga ttaccattga tccgactgta
    33421 tacggcacta ccacaacatg gacaggcaca agcggaacag acgactctcc tactttttgt
    33481 aatgtcgttg ttaatgtgcc tacacctgct aatgcaactt taacaggttc atctgtgcat
    33541 tgggaatttg atgccgatgg tgtagcatgt tcgccatttt gtaaattaaa attccttcaa
    33601 ttacgcattg gcacctcctg cggatacagc ccgtctgctg caggtgtttg ggtttgtcct
    33661 gcatgtaata ctgccggcac ttggaatcca acagttgatg atgctactac tgcagcgctc
    33721 gtatcttgtt ttacaccaca atgtgcttca tttaacattg cttttactat ttatcataat
    33781 caatatcaat gtgtaacacc tggtggatgt gtcacaacgt gcgcacatct tgatctggca
    33841 acaattactg ttgaaggaga aacagttacc accacaggcc tcgcagcagg agctactgct
    33901 tatactgttt tagattgcac tttacaatcc ggctggttat cgacaacaac acctaattat
    33961 ggtgtaccac cttatacgta tacctggtcg ccgggaggtt ataccactag tccggtgtat
    34021 gttacttttc caatgggaac aacaacgtat acagttacca tgaccgatgc ttgcggaaat
    34081 gttgctacag ataatgttac ggtaataaac aactgtgtta ccttgccaat tgaattaata
    34141 agctttagcg gctattcttt gcaagatgta aatatcttag agtgggaagc ctcttcaagc
    34201 ttagaaaaca aaacgttttt tgttgaaaga tcaagcaatg gtcgtgatta tgtttcaata
    34261 ggaatggttg aagcggcaaa tccttggcaa acaaaatatt ctttctcaga cgaccagata
    34321 gcaagtaatg tatattatta tcggttgaag caggttgacg aagatggtac tactgactat
    34381 tccaatatta tatccattca aagctctggc aatactgatg cattggtgat caatgaatat
    34441 gacgccaata caggaaatct tgcattaacc ttgttttctc cttatacagg tagtttgacc
    34501 ttgcatgtat ttgatataac aggtcggctc attgcggatt atccattcca aacccaggcc
    34561 ggagctactt ccattcaact tcaattacct tcaattgcag caggtaccta cctgctcagc
    34621 atccaagaga ccggtgtaaa tgcctcagcc cgtttagtgc gttaatgcgc ttatggttac
    34681 ctttgcgcca tgattgcagt gcagaacctc tccatttcat tcggaggtga atttttattt
    34741 gaagatgttt cctttacgct gaaagataac gaccgcgttg gtctggtggg aaaaaatggc
    34801 gccggtaagt cgacactcat gaagattttg agtggcttgc aacagcctga ttccggacag
    34861 gtaattcttc caaaagatag tttgattggt ttcttacatc aggaccttgc caaaatgaaa
    34921 ggtaagactg tgatggaaga agcgcgcaca gccttcgatg aagcattggc tttcgacaag
    34981 cgtttacatg agatagaaca tgatctgaca gttagaaccg attatgaaag cgattcatat
    35041 cacgatctga ttgaggaaat gaatgagatc gtgcacagat tggagattat agatgtaggt
    35101 tctatcgaca agcagatgga attggtgctg atggggcttg gcttcatgcg ggaagatttc
    35161 aataaaccgg tggaaacatt cagcggtggc tggcaaatgc gtatcgagtt ggcgaaaatc
    35221 ctcctcagca aaccggaatt ggttctactc gatgagccta ctaaccacct tgatattgaa
    35281 agtattcaat ggttagaaac atatttggaa acataccccg gcgccatcat tttggtttcg
    35341 cacgatagaa catttttgga taatgtaact aaaagaacca ttgaaatcgt tagtcagaaa
    35401 atatatgatt acaatgccgc ttattctaca ttcgttgaat tgcgaaaaga gcgcatcgat
    35461 caacaaaaag cggcacaaaa aaatcaggag aaatatgttg aacataccga acgcctgatt
    35521 gaaaagtttc gtgcgaaagc aaacaaagcg aaatttgctc aatcgcttat taaaaaactg
    35581 gacaagcttg aagaagtgga agttgacgac gaagaaactg ctaccatcca cttctatttt
    35641 ccaaaaccac cacgctccgg ccgaatttcg gtagaaggga gaggtgtgtc gaaatcgtat
    35701 ggtgaaaaag aagtgttgcg cttattggat tttgaaattc acagaggaga taagattgca
    35761 ttcgtaggaa agaatggtga aggaaaatcc accttatcaa aaatgatagc gggtattgaa
    35821 aaatataccg gcgagctcac tattggccac aatgttcatt taggatacta tgcgcagaat
    35881 caggcggaaa cattgagtgg cgacgacact gtttttgaca cgatagataa agttgcagca
    35941 ggtgaaattc gctcacatat cagaaccttg ttaggtgcat tcttatttgg aggagaagct
    36001 tcctttaaaa aagtaaaagt actttccggt ggtgagaaat cacgactcgc catgtgcaag
    36061 cttttgcttg aaccatataa tttattggta ctcgacgaac ctactaacca cttggatatg
    36121 cgaagcaagg atgtattgaa aaatgcactc gcaaattttg atggcacggt gatcgttgta
    36181 tcgcacgata gagatttttt aaagggaatt acgaatagag tatttgaatt caaggataaa
    36241 acgattaaag aatatatcgg cgacatctat gattatctgg atgctaaaaa aatcagcaat
    36301 cttgccgagc ttgagatgac agaaaagcaa ccgaagaaaa aagaagctgt tccggaggtt
    36361 gtgcaagaac agaaagtgca atatgccgat aaaaaacaaa aggagaaaga acttcaaagg
    36421 gtaaaaaaag ctattgaaaa atctgagcag gaaatcaaaa cccttgaggc agagataaaa
    36481 acgatggagg caacaatgaa tagtgccgac ttctataaca attcaaatac tgctgatttt
    36541 tttaccaagt acaacaccac caagcaatcg catgaatcca aaatgagcga ttgggaaaaa
    36601 ttgcaagaag agttggagag attgaatgtt aattgatcat tacaggtaat tcctttacat
    36661 aggtttcgcc ggcaattatt acatgataca agcctgcaag actttcagtg ttaatattaa
    36721 ttactgcatt tccagcgctt tctatttgat acaccttttc tccaaggtta ttgtaaatgc
    36781 ggattgtaac cggatcggta ttggggagtg ttaatgaaaa acttcctgat gacgggttag
    36841 gtgaaacttc aaaaaataaa gtctccgcac cctcaatttc agaattacaa taataggttg
    36901 cttcgctttc aaaaacacat gcatgtgtat cagtaattct ggcataatag gaaccgcatg
    36961 aagttagtgg caggtttgga tttattgctc cgggaatagg catatcatct ttaaaccatt
    37021 gcaatgtttc cacgaacgga tcaaaggcat tatcacaata caaaaaccat tgggggttat
    37081 cctgatttat tttaaccaaa ggtgtattat ccgggaagaa ataggctgaa acagtatccg
    37141 atgcaaagca aaaatcatcg tcataataat atgctgtagc cagcaagtct ccactaaatt
    37201 gagtaaccgg taaaggtgat cctgttccaa ttattaaacc ggtatttaca tcctgccatt
    37261 cagctacgaa tcctggctct ccaatcaatt gaatcgtgtc ataaaaacaa ttttccggat
    37321 ttcctaaaag gtcggtactc agtacatcct ctgataattc tcttatatga acaggcaaat
    37381 aaaatgtatc tgcacatggt aaatcataat tggcaatgta catcaattca taatctccaa
    37441 gtgcaggata agtatgctca taaatatccc atgtttgaat tataccgtca ccaaaatccc
    37501 aataatagcc ggcccatggc tctgtttcac attcgaaaaa tacatgcgta tcgttacatt
    37561 gatagatttc ggaaccgagg tcaacttctg ccatggtcgg agtacaatcc tctacaatta
    37621 aaatgatatc ccgtcttgtt ttgcttagct caatgcctaa tcgcgtttca gtaacttcta
    37681 cagttattat ataaacgccc tcagttgttg gcaatcccgt tagcattcct gtagatggat
    37741 cgatttcaat tgttgtggca gatgggaatg ggtttgttgg gcttaaagct cccaaatacg
    37801 aaagttcaga aaatggaggt ggcgtaggta aggattgaaa aaagggttct gtataatcgg
    37861 cattcaaggc aggcacgagt gcgtaactaa ggtcatcacc atcaagttct gaggcgccta
    37921 aatccatatt taatgactga tcctcacaag cgtaccaaac gggatgatta ttgaatgacg
    37981 gggcgctatt gttttcgttt cccggcacag ttgtgtataa ggtcaggcca cgcaagccag
    38041 gtaataatat attggaaatt tcttcggttc gggtattgcc cataatcact attacataac
    38101 catcagggtt ttcaggaagt gtaaccgggg tggaaaaatc gagtcgctct atatcatact
    38161 tttgaaccat tcccacacaa gtagggtaat tgctatcaac tatagatcta tctatttcaa
    38221 ttgccgcaac cgtatcaatc aacgtatagt cagtgttatt ataaataccc aaccagaaaa
    38281 caggttccaa ctcgggttca tactcgcgat accatgtcaa tttgatgata tattggtctg
    38341 cggccgtttg ctcgtaccat aactctccgc ccaatatcct agctgaaact gactgtgtgg
    38401 caaaggtcag gcataaagta gctatcaaat aatgtttcat gtagtggctg tttaggatga
    38461 ctaaaattat ccgcaaatga gtttcaagcc aatactattg ttaaaatctg acaaaagttt
    38521 ggcaaattga aacctttaat cgtatttttg cgatatacaa ttatggctta cacaaaacga
    38581 accgaattca gtgaagatga gatgcagctg gccgacatcg ccaaagcgct ctctcacccc
    38641 gccaggatag cgattttgaa ggttttgtcg gacggacaat gcatttgtgg cgatatcgtt
    38701 gacagattgc ccttggctca atctactgtt tcacagcatt tgaaagagtt aaaatctgcc
    38761 ggtttaataa aaggtgaaat cgatggtcca agggtttgtt attgtttgaa tgaaccggtt
    38821 attggaaagg cgcgtaaaca tttcaatttc ctttttacat catttaattg ttgttgatgc
    38881 agataagcaa ggcacatagt accgactttg ccaatattca tgctttacta aaagccaatg
    38941 atcttcctgt tgaaggggtg aatgaggttc aaggagctta ctatctgtta aaaaatggcg
    39001 aaaaattgat ggcggttgcg ggaattgaag gttcagcacc aacagcatta ttgcgctcgg
    39061 ttgcagtgga tgtgcagatg cataatcgcg gaattggtac gcaatttatg atagacatac
    39121 tgagtcttgc caacgaacaa ggtttccgcc aaatatatct gcttaccgaa acggcatcta
    39181 aattttacga acgactcgga ttcacaattg aagacaggtc acaagcgccg gaatccatac
    39241 gcaaatcatc ggaatttaca agcatgtgtc cgacgagtgc tacattaatg aaatatatta
    39301 tcatcaaata aaataaagat tatgcaaaac gatcaatcaa ttaaggatat ggtcaaagac
    39361 cgttacacag aaattgccac tcaatcaaaa agcaccaacg catcatcatg ctgcggcgcc
    39421 ggaggttgct caacagttga cctgcaaatc atgagtgaag actatacatc acttgatggc
    39481 tatttagcag atgcagattt gggattaggt tgtgggatac caactcaatt tgcacaaata
    39541 aaggcaggag atactgtcgt agacctggga tcaggcgcag ggaatgatgc atttgtcgcc
    39601 cgttctatag ttggtgaaga tggccgtgtg attggtattg acatgacgca ggtgatggta
    39661 gaccttgcca gaaacaatgc tcagaaatta aatttgagca atgtagaatt cagattggga
    39721 gacatagaaa acatgccgct tacagcgaat aaagctgatg tagtgattag taattgcgtc
    39781 ttaaatcttg ttccgaataa agaaaaagcg tttcaggaaa tgtatcgcgt cataaaaacc
    39841 ggaggacatt tttctgtatc agatattgtc attgttgggc aattgccaac agaattgttg
    39901 caggaagccg aaatgtatgc gggttgtatt gcaggagcga tacaaaagga tacatatctt
    39961 gacatcattc acaaggcagg attttctgat gtgcgcatca tgaaggaaaa agcaattgat
    40021 cttcccgatg atattttatc taattattta agtgccgatc aaattgcaag ttggcgaaac
    40081 agcggaaccg gtatctattc gataacagtg tttggcgtga aagctaaaac atgtggttgc
    40141 ggagataatt gttgcagcta aaatctaata cagttcaaaa accttggata aaggcgattt
    40201 catacaagta ttgttagtag catcggtatt tatggtgatc atattactga tctacagctt
    40261 tctcaactgg ccggtttggg tatcgttgct actgacagtc atttctgcaa ttggtgccta
    40321 tctggccggc aggctgatca agctatagct atgtttgcgt atctttgcct tcgaaactaa
    40381 ccaatatgat aacgaccgga aaatttgcaa ccatcttact cgcaggaagc ttattcctgg
    40441 gcgcctgtga aaattcatcg caggatgcaa tcaaagaaaa gcgcaaggaa gaaatgacga
    40501 acaaagaaaa ccgcgtcagt ccacctgcaa ccgctacggc aaatattggt gaaaataccg
    40561 tcaccatcaa ttacagttca ccgagtgtaa aaggtcgtca gatctggaat gcgttggtgc
    40621 cttacaatga agtttggcgc acgggagcca atgaggccac taccatttca tttacacagg
    40681 atgtaaccat tctcgggcaa cctttggcga aggacactta ttctcttttc acaattcccg
    40741 gagagggaga atgggtagta attttcaatt tcaatgagac ccagtgggga gcattcaagt
    40801 atgatcagtc ggaagatgct ttgcgagtga atgtaaaacc tactatgacc gaaacactta
    40861 ccgaatcact caaatttgaa gttatagcag gtgaaaatgc cggcagtgga attatcagat
    40921 tgaactggga aaaattgcaa ttggatattc ctttcgaaaa cgttgctaca aaataactga
    40981 cactaattca tttaagatcg ccggttgtta tgcaatcggc gatttttttt acatgaaata
    41041 aatcaattca acaaaggaag tagtataagc gggagtagta acagaatcaa tcaactcgcc
    41101 gagggtagga tgtgtgcatt tgatataata catatcctca tccaatccat ttatttcagc
    41161 atgacccagt gagtccgtaa tgcggcttgc atcgggataa taatctgtca ataaatcttc
    41221 aaattgggca tatattttta tggttgcccc tgctacagtg ctatcagaat aagggctggt
    41281 agcatataca tgagatacgt caaatttaat ggttctattc aagttttcgg agccattcaa
    41341 atcttcataa ataggttctt catcgcaaga aacgactgcg agcatacatg acaataacat
    41401 tacaggaaga caccttttca tagaatgcac cacgatatta ctacttaatt tgataatacc
    41461 ggtcataact gcgtaataat cattaaacgc tcttcaacgc cgataaggga caaaaaaaag
    41521 gatccttttc aggatccgga aaattaattc agcaaacatg ttatactaag gttcgcagca
    41581 cattattcat atctactttt tcggatacga cctttgctac gtcctcaata tgaatacgca
    41641 cctgttccat gctatcacgt tcacggatag taaccgtgtt atcggtcaaa gtttgatgat
    41701 ctatggtaat acaatacggc gttccgatgg catccatccg acggtatcgt tttccgatac
    41761 tatctttttc ttcataataa cactgatgac gatatcgaag gtcattaaat atttcaagtg
    41821 ccttttcggg aagaccatcc tttttcatca atggcaatac ggcaacctta acaggagcca
    41881 gtgctgccgg aagtcgcaat accgaacgca tgccgtcgtt cacctgttct tcatcgtagg
    41941 cattgcacaa catcgccaaa aacatgcgat cgcaaccgat gctcgtttct accacgtaag
    42001 gaacgtaact ctcgtttagc tcgctatcga aatactgcat ctttttacct gagaattctg
    42061 aatggcgacc aagatcgtaa tcggtgcgcg aatgaattcc ttccagctct ttaaatccga
    42121 atgggaaatt aaattcgata tcaacagcgg cgtttgcata atgtgcaagt ttatcatgat
    42181 cgtggaagcg caacatagcg gcatcagtgc ccaatgctcg atgccatttc atgcgggttt
    42241 gcttccaata atcgtatact ttcaattctt cacccgggcg aacaaagaac tgcatttcca
    42301 tttgttcaaa ttcgcgcatg cggaaaataa attgacgtgc cacgatttca tttctgaatg
    42361 cttttccaat ttgagcaata ccgaatggaa ttttttggcg cgtggttttt tgaacattca
    42421 ggaaattcac aaaaatacct tgcgctgttt caggacgcat ataaatgata tccgcatctt
    42481 cggctaatga tcctaattta gtagagaaca tcagattaaa ctgacgcaca tccgtccaat
    42541 ttctgctacc tgattccgga tctacaatat cgcagtctat gatgatctgc ttcatctctg
    42601 tgagatcgcc atcttgcatt gcttttacaa agcgagcgtg cacctcttca cgtttttgtg
    42661 taatttccaa cacccttccg tttgtggagc ggaacatggc ttcgtcaaaa ttttcaaaac
    42721 gtttgcgtgc cttttctact tccttattga ttttatcatc ttgtttggca agatattctt
    42781 caataagatt atcggcgcga tagcgtcttt ttgaatcttt attatcgata agcggatcgc
    42841 tgaaagcatc aacgtgacca ctagccttcc aggtggttgg atgcatgaga attgccgcat
    42901 ctagaccaac aatattttcg tgcatttgca ccatagcctt ccaccaatat tctttgatat
    42961 tgtttttcag cagggcaccg tacggaccat aatcataaac ggcactcagg ccatcgtaaa
    43021 tttcgctgga agggaacaca aagccatatt ccttggcatg gctcactacc tttttaaatt
    43081 tctcggcatc attcgcaatc atgctgcaaa gataatgaat gagcattcag attgagtaag
    43141 gcaaaggatt tccgagatgc agaatatgat caacacatgt aggaaatagt cacatcaggc
    43201 ctttcagcta taccaatgtc atttagaaat ccagagtgaa gggtcaagga ttacggtgtt
    43261 tttccagatc tcgagatgca cctctgtttt tccttcctcc ggatcttccc aggctatgcc
    43321 tatcttttgc tttgtagaaa ctttatcgcc cgcctttacg gtgacatccg acaaattggt
    43381 ataaaccgaa taaaattcgc cgtgttttat aatcacaccc ttttggaacg acggattgaa
    43441 gatcacattt accacttcgc catcaaaaat acttcgtact gtagcccctt cagcggtggc
    43501 gatatcaatc ccattatttt caactatgac atctttgagg acaggatgtt catgtttacc
    43561 aaaacgctca gtaatggtgc ctcgttcaac cggccaggga agtttgcctt tattgccttc
    43621 aaaatttttc gaaatcaatt gcatctccgg tgttaaaccg agattaactt tttctgctgc
    43681 aggcttttcg gcattggtgg tttttgcatt tgaagtactt cctgaatttg tggtaggagt
    43741 agttccggtc ttatttgatt cggctaactt tttggcctcc agttcctttt gctttgcagc
    43801 ttcctgagcg gcacgttcct tctccttttt tatctcttca gcaatgatgg cctgaatttt
    43861 attttttaat ccaacagcgt cttttttctt tttttcaatt tgttttttca gatctttctc
    43921 ttgcgtctgc aatttcttca cggcttgatc ttttttcgcc ttttcatcat ccagtttctt
    43981 tttctgttgc tcctcctttg tcaacaaaga ttccttttca tttttctctt tttcaatctg
    44041 agcgattttg ttctgtaggc tttcagatat ttcctctatt gccccaattt gatcttttcg
    44101 atatccatga aatgaacgca cataatgcac tctttgaaat gcatcattga tactttctgc
    44161 agaaaaaata aaatgcaatg gcttataatc ctgatcattc acataagtgt aatacaccat
    44221 tttcgcgtat tggcttttca actccacaat atccgcctgc attgcagtga tggccttgtt
    44281 ggtagttgtg agctcagcat taacaccatc aacttgcgca gcaatattgt tgatgacctg
    44341 ctcgcgcata tctattttgc ggttgagcag ttttaattcg ttgagtgagt tttccttttt
    44401 ttgcttggta ttgtcgagca gctcttcagc atctttgatt tcgtcctgca gtttattgta
    44461 tttattttga agagcgtttt tatcctgtgt ttgcgcctgc aacgacaaac tcaccgataa
    44521 agcaatacaa accaaagaaa ttttaataat aggtaagatc ccgccctttt cagttgacct
    44581 tgtatttact gttgacagag aagctgaatt cgaaaggggc atcgaattcg acattcgtaa
    44641 aaacgacatc ggctgtaaga tcttcgcgag catccagttt aatatttctg tttaatgcaa
    44701 aacgctttcc attaacttta tcgtattcgg catacaacag tgtcaggttg cgtcgggcat
    44761 acaaatcctg gataaacata cggacgatac tgaaatcggc agccatagaa agttggttct
    44821 tatacacacc tttggaggca tttagtatat aaaactcttc actctcaacg gtttcaagtg
    44881 ttgtgctatc gtacattacc gggttgccga tgataagatt ttgcagatcg ctgaaattga
    44941 ggtccagatt caggctgtcg cgcaaaaaag tgtaatctct cggaagatat tcctgattca
    45001 gcctgtcaag gatctctata gaatcggggg tgatgcggat gcgggcgcct tcaacaccta
    45061 aaaaacgcaa ggaaatgccg atcagtgaat cgcgacgaat atctatagac gctgaaaaac
    45121 tttggtttcc ttgatttgat tgaaggtcaa ttttagcttt agaagaaaat gtttcgaatt
    45181 gaataagctg actgcgcagt tctttatcca actcttcggc agtcattggt ttaatttttc
    45241 cactaaaaag cgcatgtgat gtgccgcaag cagaaagcga gaaagttgca atcaataata
    45301 tgaaatatgc atttttattc atacaactta ttatcattga tttttttgtc caacatatct
    45361 gaagcatcac ctgctgcttt agctttcttc caatattcca tcgccttagc ctcattaccc
    45421 aagcgatatt ggatatcgcc ataatgttct ataaccgtgc cacttttatc accacccttc
    45481 tgcatggctt tttcaatcca tgcagctgca tttttataat ctccttgctg atataaaacc
    45541 catccataag tatccaggaa agatggattc tcaggctgca atgttgtggc tttttttgcc
    45601 agttctgcag ctctttcgag tttttctttt ctcagcgata aataatatgc ataattattc
    45661 aaggcataaa catcattagg attgaggttt aatgcattct cgaatgagtc atcggattcc
    45721 gggaactttt tcagttcatt atatgcatca ccaagtgcgg aaaacatgcc ggatcttaag
    45781 tcgatattcg agatagtaag cggcaatcca cgtcgcagta cttttacggc atcttcaaat
    45841 ttatcctgtt gatttgcagc aatgcctgca aaataataac tcagcggttg gttcggatat
    45901 agttcaatcg ccatattgga aaccaatgcc aacgaatcac ttttatgcaa gtcggaataa
    45961 atgtaaaaca atttcaacca cacatcataa acatcgttgc gcaaggcaat ggatttattg
    46021 tacacatttg cggccgactg taattgctca tcgtaataaa ggaaatctcc tcgcattgca
    46081 tatgattttg catcatcagg atgtgcttct acgagcagtt tcgaccaatt aaacactaaa
    46141 tcctttgttt tgaattcagg cctgccaatc gaatctataa aaagtaccag ataaaatatt
    46201 ttggtgtcaa tatttccatc cgggttagca aaggcattat tcatcgtatt cacataatca
    46261 gcatactctc ctgctttttg ctgcatagag gccttcctga actgaaaatc ggtattggat
    46321 gggtcgatag ctagcaaatc attaaatgtc ttttctgcta attcaggttg accatttctg
    46381 tcgtaatagt cactcagata tcgcaaatag agtgtattgt ccggattttg tttaatgagt
    46441 ttttgcaacc aggaaatggc attattgtat tgttcaacag ctgcatacag tcgctgaatt
    46501 tcaaacatta cctgttcatc atcgccaatt attttgtgca gatcaagtag cgtagaaaca
    46561 gcctgttgcc tgtcgcctaa tttttcatac gttgcggcta attggtaata agtatcctca
    46621 tcgccaattt catttttgat catgccgaga tacacatcaa cagcttttct tccctgaccg
    46681 ctatattgca atattccggc atattgctgt tgatagtcgc gatttccggg gtcgatggaa
    46741 gctgcctttt cgctatatgc taatgcatct ccaatttgtc cgtgattggc aaagatttta
    46801 ccgagttgat acattgccgg tgcattattc ggatctatct ttacgatatt attaaaaata
    46861 tcaacggcct tattataatc ttcaagagca acaaacttac atgcatcaat atactgacgc
    46921 tcgagttcag cagtatcaat tccttttttt ggtcgcgatg cagaggaccc gacatttacg
    46981 acgcttcccg tttgttgttg tgcttcttta ggcccgttgc aagccgcaag aaacatcaag
    47041 gttaacgcgc ataatccaat ttgaagtcgt aatttttttc tcatgccata gtactgtaat
    47101 cgctcacact caggtcatca ggtgttccac taataacagc aaaacttccc accattgaat
    47161 tggcgataca cttgtcggtg atactaactg attgttgcac aatggaattg cgtattaacg
    47221 catttttaac aac
//
LOCUS       DAOQHY010000004        46876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_282_length_46876_cov_5.866363, whole genome shotgun sequence.
ACCESSION   DAOQHY010000004 DAOQHY010000000
VERSION     DAOQHY010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 46876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46876
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_282_length_46876_cov_5.866363"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /gene="era"
                     /locus_tag="PLL28_00720"
     CDS             <1..691
                     /gene="era"
                     /locus_tag="PLL28_00720"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNF67860.1"
                     /translation="DQPEYKLHQWMNAQVSTALKDGDVLVFMTDPKDELHADHPLIQT
                     LQQIRTPVIVVINKSDIIAKNVLEQLMIKWTSVLPKATIISISALLKINTDKLFQHIV
                     DALPVHPPYYDNDTWTDQTERVIAAEIVREKIFMQYQQEIPYNVEVIVDSYKDKGDII
                     AISAVIYVGRDSHKSIIIGKSGSAIKNIGIAARQDIEKFAGKKIFLELFVKVRENWKN
                     DDRGLKHFGYE"
     gene            complement(747..2285)
                     /locus_tag="PLL28_00725"
     CDS             complement(747..2285)
                     /locus_tag="PLL28_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF67861.1"
                     /translation="MKLRYLPLMTLGLLIGSISAQNVDHPYAFGFGAHFIDYNIEDDG
                     YTKDLFDSADINGAWVANRYTFGGSINNSFSMEASLAAASISTISKGNIRETFLWCGV
                     DLGLRYQFANGYMLPHDSWFAPYLAGGVGANYLDSDDPDATTLFPEGEIAIGTNLWLT
                     PMFGINLQTGYTHQFSETGSDYMQHAVGLVIRFGKGEDKDGDGIANWEDSCPDKAGIP
                     LFNGCPDTDSDGITDAMDACPTENGLLQFNGCPDSDSDGLADKDDACPTEKGLAKFSG
                     CPDSDNDDIPDKDDRCPKDKGLKEFNGCPDSDGDGIADLDDACKQEKGLAKFGGCPDT
                     DGDGIMDKEDRCPRERGEIALKGCPDSDGDGVADIDDKCPDKPGIKATGGCPVIEEET
                     KKAIIEKINFAAKSIQFESGSDVIKKSSYPVLDQIVAIMILYPGTNWSIEGHTDDQGD
                     DKFNQELSDKRAAAVKNYFGSKGVADARLSSAGFGETKPIADNKTSAGRAQNRRVEIK
                     LVEQ"
     gene            2442..3380
                     /gene="trxB"
                     /locus_tag="PLL28_00730"
     CDS             2442..3380
                     /gene="trxB"
                     /locus_tag="PLL28_00730"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNF67862.1"
                     /translation="MHMERVKCLILGSGPAGYTAAIYASRANLQPVVYTGIEPGGQLM
                     QTTDVENFPGYPQGIMGPELMEDLKKQAERFGTDVRFGIATRVELNDRVKTVHFDDGT
                     SIEAYTVIISTGASAKWLGLPSEKRLNGNGVSACAVCDGFFFRNQEVALVGGGDTACE
                     EALYLSKLCTKVHMLVRKDSFRASKIMQERVLNAPNITVHWHSETLEVLGDQTVTGIR
                     IRNNKTNEETVINITGFFVAIGHQPNTQIFKDQIDLDEQEYIITKAGTTKTNIEGVFA
                     AGDVQDKHYRQAITAAGTGCMAALEAERYLTEKGIH"
     gene            3438..3953
                     /locus_tag="PLL28_00735"
     CDS             3438..3953
                     /locus_tag="PLL28_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNF67863.1"
                     /translation="MRSNTFPSIHALNVTALSGRTFSMSEYTGKVLLIVNTASKCGHT
                     YQLGLLEELYQEYKDRGLVVIGFPSDQFAQEPLNGEQIQEHCQVNYGVTFPMMAKSKV
                     RGDEANAVFKFFSSKSLNGKFSKQPRWNFYKFLVGRNGEALDHFWTYRKPNNAALRKA
                     IERALMATNPK"
     gene            3973..4689
                     /gene="bshB1"
                     /locus_tag="PLL28_00740"
     CDS             3973..4689
                     /gene="bshB1"
                     /locus_tag="PLL28_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HNF67864.1"
                     /translation="MKLDILAFGAHPDDVELGCSGVLIKEVRNGKKVGIIDLTRGELG
                     SRGTAEIRAQEAADAAQIIGCSIRENLELPDGFFEDNNANRAIVIQAIRKYQPDIIIC
                     NAIHDRHPDHGRGSNLLESSAFLSGLIKIETTENNIAQKPWRPRLVLHYLQDRWITPD
                     VIVDISDVWEERMESIAAHRSQFFDPGSNEPETYISSKNFFEAIESRAREIGRPAQFK
                     YAEGFTCSRTIAVKSLGELM"
     gene            complement(4776..5138)
                     /gene="rplS"
                     /locus_tag="PLL28_00745"
     CDS             complement(4776..5138)
                     /gene="rplS"
                     /locus_tag="PLL28_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNF67865.1"
                     /translation="MEFLQSLAQDLIPSKEIPQFKAGDTVTVNYKIIEGDKSRTQAFK
                     GDVIQRKGTGRTQTFTVRKISNGVGVERVFPLFSPNIESIEVNKSGKVRRAKIFYLRG
                     LKGKSARIKEVKSTSVAE"
     gene            complement(5202..5882)
                     /gene="trmD"
                     /locus_tag="PLL28_00750"
     CDS             complement(5202..5882)
                     /gene="trmD"
                     /locus_tag="PLL28_00750"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNF67866.1"
                     /translation="MRIDIITVLPELLESPFQHSIMKRAADKGLLEVNLVQLRDYGLG
                     QHKQVDDYQFGGGAGMVMMVEPIFNCINALKAQRIYDEVIYLTPDGERFNQKIANQLS
                     LKGNIILLCGHYKGVDERIREHLITREISIGDYVLSGGELAAAILVDAIGRLIPGVLG
                     DETSALFDSFQDDLLAPPVYTRPANFQGWEIPEILLSGHTAKIEEWRHDEALKKTKSR
                     RPDLLPEN"
     gene            complement(5894..6400)
                     /locus_tag="PLL28_00755"
     CDS             complement(5894..6400)
                     /locus_tag="PLL28_00755"
                     /inference="COORDINATES: protein motif:HMM:NF013907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67867.1"
                     /translation="MQGELVEIGYVIRTHGVKGHVRIAFDDNCKELSVSEALYFLIKG
                     VHMPHFISEISHFNNGDALVLLEELHNKEEADLYTKRPVFGPAEWLEDTDEDQEIAFE
                     GWKIHDETYGELGQIIASADMGAYLLLTVSHQGRELLVPMHDHLIIREDESSETLFTK
                     LPEGLTDL"
     gene            complement(6410..6976)
                     /locus_tag="PLL28_00760"
     CDS             complement(6410..6976)
                     /locus_tag="PLL28_00760"
                     /inference="COORDINATES: protein motif:HMM:NF011094.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNF67868.1"
                     /translation="MATKIRLQRHGRKKAPFYHIVVADVRAPRDGKFIERLGSYNPTT
                     VPAQIIIDVDKAVDWLQKGAQPTTTVNAILRYKGVLYKKHLLRGVAKGAFTTEVADQK
                     FDEWVKSHETAVIDKVKAIEASAAATKAAEVARINAKRLEEASKKAAAEREAEAAATV
                     ETATEEAAVEETTSEAANDATTEETTNE"
     gene            7102..8541
                     /locus_tag="PLL28_00765"
     CDS             7102..8541
                     /locus_tag="PLL28_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HNF67869.1"
                     /translation="MAQENSERDLSARAVKILRWHEHLISFFQQYLNEKQFVTISGVL
                     IGITSALAAILLKAMVFYIHALLFTESNIGKISPYIYSSFPLLGILVTTWIVQRYFGG
                     NLGGGNAAIIFSIKKKESRLPGKLMYSHLITSAITVGTGGSTGLETPIVTTGAAIGSN
                     YGKTYKVGPEERTLMIASGVAAGIAATFNAPIAGVLFAVEVLISEITIVAFIPILIAA
                     ASGAILSKIILNEEILLNFSNMAPFNYHNVPFYILLGLLSGVIAVYYIRTSLRIERIF
                     RRVKQRFYVQRAIIGGILLGILILLFPPLFGEGYESIKTLAGNHAEDILKGSVVVGNL
                     QDPVMMIIVLTFVLLLKVVATSITLGSGGNGGNFAPSLFTGAYLGYIFAGLINLTGIF
                     NLPPGNFTVVAMAGMLSGIFHAPLTAIFLIAEITGGYDLMVPLMIVSAISFGVSRYLF
                     TYALDGLKLNSMLQQAAREEQEENKAKTE"
     gene            complement(8542..9489)
                     /locus_tag="PLL28_00770"
     CDS             complement(8542..9489)
                     /locus_tag="PLL28_00770"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNF67870.1"
                     /translation="MAAKPTFLIVGAVKAGTTSIHEYLQMHPEVYMSPVKETNHFSDA
                     DMLFDHFNVDYKQDINANLEKYLAGPMDKKIHIAHVRTAEQYAALFKNVTSQKAIGEV
                     SNSYLYLPSTAAAIHKALPDVQIVMILRNPVERLYSQFLMNLKLGKIVEKDLLKEIEA
                     DQVKPVKGWGVSHLYLEVGMYYEQVKRYLDIFDKNKVCILYYDDYKKDPTGTMQSLFK
                     FLGVDATFTPDMSKRYNEAGMPRFGKLNYWLTQIGVYGLVKRIFPSNLKERLKSLIYT
                     KENIPTITAEEKEYLINYYRKDIEQLQLLLNKDLSNWLK"
     gene            complement(9496..10227)
                     /locus_tag="PLL28_00775"
     CDS             complement(9496..10227)
                     /locus_tag="PLL28_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67871.1"
                     /translation="MIISHKYKYVFIELPQTASSAIARELKENYDGHEILFKHALYST
                     DFLKQAKPEEKQFTRVSGLRNPMDICVSNYFKFKTDHENRYSNPRLMKHGFLRKYIMR
                     WWNVRQYKSIIEGGENFENFFMRAYTLPYASWSIIEHKRMDYVIRFENLQADFAKALE
                     LIGVDKVRDIPVANKTAEKSKTFWEYYESDKAKRRAKYIFGPYMQRWGYDFPESWNHI
                     RIPWHSFFLYRIVNVARIIFWKFLR"
     gene            complement(10224..11264)
                     /locus_tag="PLL28_00780"
     CDS             complement(10224..11264)
                     /locus_tag="PLL28_00780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNF67872.1"
                     /translation="MRPKVCVVLVNYKTWKDTLECMESVLRSNYTNYQIVVVENGSGD
                     DSWEQMLKWARGENAIDITTQNPLFHLTHPPVHKPLLYFRASPEEAAHMRLGPLDGIM
                     PLLFIKSDENRGFAGGNNVGMRYALNSEFDYVWLLNNDTVIEPNAIEELVKYCEVKKE
                     KKQTIGIVGSKLMVYHQPQKMQGIGAIFNKYSGKSKIIGAQEIDKNQYDEQEISCNYV
                     IGAAMFVSRPFLLDVGLMSEEYFLYNEENDWSARAKLKDWEVGVAVKSKVYHKQGAST
                     GNSPKKSKWQIKALHYKYRGKIKLYKKYYRPQLPFLYLHLITRSFKYVSKGNFSEAKV
                     IYQAILNTKFES"
     gene            complement(11272..12231)
                     /locus_tag="PLL28_00785"
     CDS             complement(11272..12231)
                     /locus_tag="PLL28_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNF67873.1"
                     /translation="MTTGKTILPTFLVVGANKGGTTSIYHYLKQHPEVYLSPIKEPHY
                     FSKDIDVNQFKKEFAHNKLQDIEKYVQGDMKQEFHAAFVRSEEQYLKLFKNVTSQKAI
                     GELSTSYLYSTVAATEIKKLIPNCKIIICLRNPIERAYSHYRMNLWTGNSNEFDFHKA
                     LLEDYQHQPKVWGNAHLYTEIGMYYQQVKRYLDTFGAENVKVIFTEDLKKNSAQVIRD
                     LYTFIGVDPSFNPDTSTQYNEVYTPKYKNLTWFLNKSGIRPFMKRISPKFLKGAVVKL
                     FYKQKADKGQMPPAAKSFLLEKLGPDVHALESLLNKDLSGWLK"
     gene            complement(12235..13230)
                     /locus_tag="PLL28_00790"
     CDS             complement(12235..13230)
                     /locus_tag="PLL28_00790"
                     /inference="COORDINATES: protein motif:HMM:NF027586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4915 domain-containing protein"
                     /protein_id="HNF67874.1"
                     /translation="MQLIHPDKRILVIGSLNANPADRAISRQDIRSMQVIDENGKIHP
                     VNFFNTWGELGMSSMCFHPNDPFVFYLASSSEVFKFMLKENRYEQLDIPNLTDIHEIS
                     MIGEQVWISNTKHDEIVSYNIRTNRVDQRINLSDFKSNTPEEGEGEADVEVVDKFHCN
                     LIFEGYEGDLYILVHHTSGRQLMKRIAQKFIKSQGNGGVVDITEKKRYPLNFKAPHSV
                     RRINNQYWVFDSGHFELKVFDKNWNFLRTMDTKGFGRGGDLDVERNVYYAGVSETRKR
                     YLGLFPGGEAVPNMVQVFNINDYKLAGSIKIPNIEQINNVYCLSKEVADKLLQLK"
     gene            complement(13240..14574)
                     /locus_tag="PLL28_00795"
     CDS             complement(13240..14574)
                     /locus_tag="PLL28_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67875.1"
                     /translation="MGILTYEKGIYKEPMSVFGKTMILVVFILFTTRILTRTPLVDYY
                     MPFEILWYTFIITFTFIYAMYIFLVRKTVDEIHYYYILIIIMPLWAGFMANLSEGQNV
                     MGGISSQRHWYGISGIFVLIYLLQTKFFTIGDLFRAMKWLGWGTMAYFIWCYIFLDPK
                     QYLDYSFVGFSDIKGGYRFKFGIDFIAFLSVYYTVKYIKQKDSVSMWLSAANLAYLFF
                     LHQGRSVMISVVATLFVFYMFEVNWQRAFRIFTSIGIGFFVIMGILYAAIPDRINDYV
                     IQYTSLATVVFTGNASGESSVDARLEEMAILFVYLQSYPIGWWIGIGKLSAESAVAGE
                     HTIATVPPGDIGILGSIMTYGILGSVLIYLQFLIAFRSVKFIRRYKDEPFMIASKYFL
                     IFCFLSSLAKGYLFMQPGSTAIFILVIYAYKLLEVQMDRQGLPETAVLKNSF"
     gene            complement(14576..15454)
                     /locus_tag="PLL28_00800"
     CDS             complement(14576..15454)
                     /locus_tag="PLL28_00800"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNF67876.1"
                     /translation="MSGIKQNFICIGAQKAGTTTLSDILSAHPDICIPPIKETKFFLF
                     DQDYQKGKSFYDQYFSNYRTQSAVGEFDPDYLLFPYTAERIAETLGTDIKILVVLRNP
                     ADRAYSHYLMTKRKGLEPHDFEAALQAEASRKEDLKTRKIFAYIERGMYGTQIAQFLK
                     VFPAENFLFLVFEEDFIKNRQGTVEKVQAFLGVPFKALDVDLHSNEAGEPINDSLNEL
                     VRKPNFAKRILKTILPFKAIRKWMRKAFISQNMQKVSSPKLSEEQRRQLIADYFIQDI
                     HLTEKLTGRDLSLWYN"
     gene            complement(15456..16361)
                     /locus_tag="PLL28_00805"
     CDS             complement(15456..16361)
                     /locus_tag="PLL28_00805"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNF67877.1"
                     /translation="MTQRWPNFLIVGTARAGTTSLHEFLGNHPDIFMPLQKEPCFFTY
                     VDEQPEFKEARHRYLTNPAEYLAMFEHHNEAVMGESSTPYLYFHQKSIANIKRLVANA
                     DEMKILIVLRDPSERAYSQYMHNRRDLREPLTFEEAIDAEKQRMADNWHFDFFYVDKG
                     YYYQQVKDYIDNFKHVKIVFYDTLEKQPEKLLSDIYQFLGVTQLQEAKMVKRNQSGEM
                     KVKWLKQMMTTRKNPILNFFRKLMSRDTKKKLRNWAKDKLLRYNLKKTEMNPETRRKL
                     IALYSEDIKQLQTLVQKDLSDWLRV"
     gene            complement(16358..17869)
                     /locus_tag="PLL28_00810"
     CDS             complement(16358..17869)
                     /locus_tag="PLL28_00810"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HNF67878.1"
                     /translation="MIRKITKKFRRKLKDQDFKEIFSKGISGTAISLVGRFSGYLLLM
                     VIGWVYGQEAMGVYTVGLSILSIVGIFGRFGVDMAMSRFVAQYGGSGRWDLIRHTYSV
                     AFRIVFFLGLFLSAATFFIVPYFTDNLFDTSSPNYDQYTFFMQIFAVTILFYVLGGLS
                     EECIKGLKKTRQYSWISQTINQVAAIAFLLLFGLFSKDELWVNLSYCLGVFVAFAIAQ
                     YYWIYYLKHPEVGGSEFGRKKDKKEKKPAKDNAAAAVLAVAQQEQPINTGEMLKLSAP
                     MMMAKYLTMIYTWLDILILAWYAPEAMVSIYRVATRLTSLSTIPLVSINSIAGPKFAE
                     AFGKNDQRSLRNNVKQATRMIFWTCIPILAIFLIFPKFSMWTFGKDFTGSEAVTVFVI
                     VSLGQAANILTGPVTVLLNMTGRQNVTMYYALATVVIDVSLNLILIPIYGIIGAAIAT
                     AVARTVLNVGCALQIYFTMGISTIYNPFADLWSVFSKKKMQPVINESKNEEEI"
     gene            complement(17866..18996)
                     /locus_tag="PLL28_00815"
     CDS             complement(17866..18996)
                     /locus_tag="PLL28_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67879.1"
                     /translation="MKKLILRIGLLLVIVFAICQLILMSMPFSWGNTRLNTKYTTYSE
                     NPEQYNTLLIGASTTYRHIDPKQFDAAVNAQYPDLHIKSFNFGIPANRTPQSMYMLEN
                     LLKEGNTDHIKYVVIDLSELTKMGADNLHKKEMLYWYNWGNIGDVMKASYESEKGTAN
                     KFGVPFLHAFSFGEKSFLVGMGPSVIMQHAGLNIEPLSVGPDKNGFYSLDQEMHDDPE
                     GDLAIRYNDLRTQDTIDFRTQRCQMLYDKFKDAKKNPNKTMEADLLKIIKDCEKQDIS
                     VVIMLSQRLGDRYEYLIPLYNRIPEKNRIGFQNPSAYPMFNERENLFDLAHLNSSGAK
                     LFTDIFADQWIDRLIMQGKIAPRPVAPLNSLQQTNSGTGDAL"
     gene            complement(19000..20499)
                     /locus_tag="PLL28_00820"
     CDS             complement(19000..20499)
                     /locus_tag="PLL28_00820"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNF67880.1"
                     /translation="MIFNSLQFLIFLPVAIFLYFSIPYKWRWLFLLFSSYYFYMSWKA
                     EYAFLIVFSTTVDYLTANAMAKQTDKRKRRPYMLISLFSNLGLLFSFKYFNFFNDATR
                     AVFDSFDIFYNVPEFNLFLPVGISFYTFQALSYTIDVYRGDVKAEKHFGYFALFISYW
                     PQLVAGPIERPGDLQPQLKSNHDFDYDRVKKGLTRILYGFFKKVVIADRLGIFVQAVY
                     GQSNGMGGWNYPSFEHGGWPVVIATWLFAIQVYCDFSGYCDIAIGSAKIMGHNLTDNF
                     RTPYFSKSIREFWERWHITLTVWVRDYLYIPLGGSRVSFGRMLFNNWFTLTVMGFWHG
                     ANLTYVMFGFIHGFYIVVSRVRDKYMPKMVLAKWIPNMPILVNVLLMIWIFNLTCIPD
                     IFFRSNSISDAWHIIGQMFKASTMHEFIYNSAPKGAVPSVIEFWISLVFIAMLLFIDY
                     NLYIRKSATIEDQVAIRPWYTRWSFYVIFLVIVACFAVTTKSAFIYFAF"
     gene            complement(20526..21671)
                     /locus_tag="PLL28_00825"
     CDS             complement(20526..21671)
                     /locus_tag="PLL28_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67881.1"
                     /translation="MQYDRPYNDEINIGDLFTKFGEYRRYLVKKWWVILIAAILLAIG
                     LRFFAVWKKEYYISHADFAVKGTEGSSTSSLASLASSFGIGITTGTEFTNELFLGILQ
                     SRTLVKEALMEKRTLTVKKGVAPREDYLANFYVEMYPRWAKKKKVKNFKLEHGSLDSL
                     TVYEDSCLTVIYDEIIENDISTEFSDDLGMNQMEVSSLSRDFSYYLADYLAYAGSNYY
                     INAQVVNEQKTVNLMSEKADSLRALLDGKEELLARIQDGSAYQVKLSGYVNQNRLIRE
                     IGLITTEYSTTYAQLQLALFDLQNKTPLVDMIDQPKFATIKEKEQTTMFMIIGFILGT
                     ILTSIVLAFRKYIRDSVEEARQKQLVIEKYHKEQESQSLIDTDSNKI"
     gene            complement(21702..24098)
                     /locus_tag="PLL28_00830"
     CDS             complement(21702..24098)
                     /locus_tag="PLL28_00830"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNF67882.1"
                     /translation="MKKFSSITLLLLVVGFTQTLYAQSTPIVPGVSSSTLESLGFDSA
                     EIDAIMSTTGSSTSTAVEAAAQIQDAQQSAVQAANNQLNTLAPNTATLPPPVVEQVIE
                     QAISNDTPSSAPAGIWGQDIFRNGSIDLFDKVPNAKVSDNYIIGAGDQINVSIYGWAY
                     YNGNLTVSEDGYITTVETGRIYLKGLTFSAARQLVRQRFASSFDLVHSTFDMTLTYSK
                     TIKVSIVGEVVKPGTYNISSVNTAFNALVAAGGVTDIGSVRNIQVKRNGKVIKTLDVY
                     QFLMNPGSTDDTYLEANDYIIVNGIGRVVDVQGEINRPLKYELVKGENLNELIAYAGG
                     LRSTAYKRNVTIFRYINNENVVLDFNLDSLERERVNFPLLDGDKITFARIPEVVENIV
                     TIQGSCRFPGNYQLTEDMRISDLIAKAKGLNYDAYTDRAYLIRKNDKLNDVYIPFDLQ
                     EVIDNPNSAFNFKLTKFDVIDIFSKQEFRETFNVSISGSVKVPGIFPYYEKMTLKDLL
                     YYSGGLKVEAANSRIEISRIVNFNEANSDNEPTRVVIQSISIGKQLELEDGANTFQLQ
                     PYDQVFVRNTPEFEYQKNVTVLGEVKFPGVYTLTSRTETLADVIERAGGLTQWAYAEG
                     ATMNRNDVASTLVFLHKALENPESKYNYVLREGDVITVPKAGDLVALKGEIKYPFVGD
                     PGTVIVPFEKGRSARFFIKKYGKNFDDDAKRSETYIVEPNGYVRRTHNLLFIHFYPRV
                     KVKGCQVVVPQKPEEKPDAPEAPSAPREPFDWNTFATTLSAGILSFATIYVLILKVPS
                     "
     gene            complement(24263..26341)
                     /gene="metG"
                     /locus_tag="PLL28_00835"
     CDS             complement(24263..26341)
                     /gene="metG"
                     /locus_tag="PLL28_00835"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNF67883.1"
                     /translation="MQEPKRYLITSALPYANGPLHIGHVAGAYLPADIYVRYLRKQGK
                     DVVFICGSDEHGAAITIQAKKENTTPKAIIDKYNAIISKAFSDLGISFDIYHRTSAPI
                     HHETSQEFFKTLHDKGVFEERESEQYYDEEFKQFLADRYIKGTCPVCANPNAYGDQCE
                     KCGTSLSPTDLIEPVSTLSNKPPVKRKTTHWYLPLNKFQDWLQSWIVEGEGRTEEWKK
                     NVLGQCASWLKEGLHPRAITRDLDWGVKVPLPNSEGKVLYVWLDAPIGYISATKQWAL
                     NTGKDWEPYWKSADTKLLHFIGKDNIVFHCLIFPCILKSHGEYILPSNVPANEFMNLE
                     GDKMSTSRNWNVQVHEFLEEFPGKEDVLRYVLCANMPETKDSEFTWKDYQARNNNELV
                     AIFGNYANRVMVLTEKYYGSKVPAQVDSVEQDAYVVAYRAQVAQLFNDYCTGIESYRF
                     RDALMAFMDIVRLGNKLLTDFEPWKLYKTDPIRTEGVLRICLESLYDMATLCEPLLPF
                     SAGRLNAYFNLPAAPKMAVGGVNLTSGARLGSPFYLFEKIEDQVIQSKLDALESIRRS
                     REAVTAAETTIIAANPIKEAISYDDFSKLDLRSGVVLSAERIAGADKLLKLTVDLGFE
                     VRTIVSGIALHFSPEALTGKKVVVLANLLPRKMRGVESNGMILMAEGKDGALHFVAAE
                     GNAGPGDSIS"
     gene            26378..27277
                     /locus_tag="PLL28_00840"
     CDS             26378..27277
                     /locus_tag="PLL28_00840"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNF67884.1"
                     /translation="MRNSMILKQGDKIGLVATARWIEQETVDIAMAQISEWGFIPVVG
                     KHALSKNGQLAGTDKARASDLQMMLDDNTIKAIFCLRGGYGTIMIIDRLDFTLFTQFP
                     KWICGFSDITILHAHINDKLGLPTLHCSMPYSFPTNTSDAIAQINAILKSESITISSE
                     ATSYNKIGEASGILVGGNLSILYSLLGTRYGFSTAGKILFIEDLDEYKYHIDRMMMSM
                     KLAGKLDHIKGLIVGGMSDMKDNPVPFGMEIEEIILEHVAHLNIPVCFNFPAGHIEHN
                     LPIRLGTQAALSVTTSGGKLSFL"
     gene            complement(27354..28202)
                     /locus_tag="PLL28_00845"
     CDS             complement(27354..28202)
                     /locus_tag="PLL28_00845"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNF67885.1"
                     /translation="MIKPLLITALSFSALVPGTNEDFSSGRVVTPSESCEIPHMAFQG
                     GESLTYKVYYNWGTLWLSGGEVYFKLTEESFKGIPVLHASGEAISYKSFDWFFKVRDK
                     ADTYMNKQTLQSYKFSRDVYEGGYTFYRSYDWDREKNIIYSYQDNHKGKKETKTIYNA
                     DPCGVDLLSTFYWIRTIDYSGLKKGDKVPVKMAIDDAQYDMYVRYDGKEVYETKLGKF
                     NCVKLKPLLQEGKVFKEGEGMTIWVTDDQNRIPVRIESDLRVGRITCDLKSYGGNKFP
                     FTSKVN"
     gene            complement(28199..29179)
                     /locus_tag="PLL28_00850"
     CDS             complement(28199..29179)
                     /locus_tag="PLL28_00850"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HNF67886.1"
                     /translation="MKTTNKKTLWWSIAIKVVVFGILLFALYKQVYLNENVHAIPEQF
                     LSIGAGKAIGYGILLVILMIMNWGFEAWKWKKVIDNITPIKFIRAFKAVWTGVTLGLF
                     TPNRVGEFGGRILYVPRKFRIKGAIVSLIGSFSQNLITVIAGIAGLLWYVYAIEKIDL
                     LVTGALALVAVIAVILLLLAYYNLDVMVNLFRKAKFLKRIYPYTTVLGDYSANDLSRL
                     LLLAFLRYSVYAAQYLILLNVFGAELSVMTGLAAIAVIFLAQTVVPSFAIADLLTRLP
                     VAALILSKCDVSLQISLAATTSIWILNLIIPAIAGYIFIIRFNIFKNRQS"
     gene            29192..29755
                     /gene="ruvC"
                     /locus_tag="PLL28_00855"
     CDS             29192..29755
                     /gene="ruvC"
                     /locus_tag="PLL28_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNF67887.1"
                     /translation="MAAASEKKIILGVDPGTNILGYSIVSADKNTIKIIINGVIQLKK
                     TDDHFTRLSIIFEKLSTYIKEYQPVVMAIEAPFFGKNVQSMLKLGRAQGAAIVAAASN
                     GLEVIEYSPRRIKQSITGKGNASKEQVAKMLQSMLQLESMPKYFDETDALAVAVCHVN
                     ASKRPAVAGKQYKGWSDFIQANPGKIK"
     gene            complement(29757..30149)
                     /locus_tag="PLL28_00860"
     CDS             complement(29757..30149)
                     /locus_tag="PLL28_00860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNF67888.1"
                     /translation="MPSIFTRIINGEIPCHKIAENDKYFAFLDIMPLAPGHVLVVPKV
                     EVDYIFEMEEDDLAGILPFARNIAIAMEKAIPCERIGISVIGLEVPHAHVHLLPLRTM
                     DDINFSRPKLKMSQEELASIAEKIRSYL"
     gene            complement(30151..30624)
                     /gene="greA"
                     /locus_tag="PLL28_00865"
     CDS             complement(30151..30624)
                     /gene="greA"
                     /locus_tag="PLL28_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNF67889.1"
                     /translation="MAEVSYLSKEGMDNLKAEIQELKTKGRAEMARQIKEAREKGDLS
                     ENAEYDAAKEAQGLLEMRIAKLEELLANARILDESKIDTSKVSVFTTVKLQDIKFKRE
                     IKYTLVSEKEADVKSGKISVTSPIGKGLLGKKVGEVVKITVPAGVLEFKILEISM"
     gene            complement(30699..31751)
                     /locus_tag="PLL28_00870"
     CDS             complement(30699..31751)
                     /locus_tag="PLL28_00870"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNF67890.1"
                     /translation="MKRILIALTLCIPAGVFAGNPDRSGEAGASELLIDPWAQSSGWY
                     GLNIASVRGVEAPNINVAGMTFMDGSQLEFDRTEWLVGSDISLNTFGFATKVGDGALG
                     ITLTSMDLGEIEVTTTDNPEGTGAVYKPRFSNFGISYAKRFADYLSGGLTVRVISESI
                     SDVKAIGVSMDVGIQYISGSETHPEQIKMGIALRNIGTPMKYSGDGLSFRGNVPDGDY
                     SMTQEFRSESFELPSLLTIGLSYDFYFGTHNRLTAVGNFTSNSFYKDQFGAGLEYGLK
                     VKNTEMFMVRAGYRYENGITSDVDRTTASTGIAAGVTVQYPIDDLGKNLVALSYSYRT
                     AQQYQGSHCFGLRLSF"
     gene            complement(31776..35651)
                     /locus_tag="PLL28_00875"
     CDS             complement(31776..35651)
                     /locus_tag="PLL28_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67891.1"
                     /translation="MLHKKQIWIVMLMLFLGFTAQAKENAGITGKRSTNYARLNAGCA
                     DATTQIDLDINNVRARLLGAGDFWWDLQNDAKYEIPKVDPSTGVIPVSSSFAGALWIG
                     GIDAGGQLKIAAQTYRQSGNDFWPGPLDADGTIEEATCNAYDRHWKLNRTDVDDFFET
                     ILVYGGTPGVSQTASVIPEGEIPQIIREWPGVGNQYATGKAGELLDVQKDLAPFVDVN
                     FDGIYSPELGEYPDIDGDQAIFWVYNDKGNIHTESGGDAIGIEIQAMAFGFQTNDEIN
                     DMTFYRYKVINYATTALDSTYFGQWVDSDLGAYDDDWVGCDTTLSLGMCYNGDLVDGP
                     TSPNYGANPPIFGTDFFQGPIKYITEGGSIVDSVRLGMSAFLYYNNDFSIIGNPEVAA
                     HYYGYMSGTWKDGSPFTYGGNGYGGTTLSNFIFPSDPSDPTGWSECTESNPAADRRYL
                     QSAGPFTLFPGAKNEIVVGAIWVRPPVSGGCQTSFDLARLADQKAQALFDADFQLIGG
                     PDAPDMDIRELDQSIVLSLTNGVTSNNQGESYQETDPLIVSIVSQLPDSILEANPGAT
                     DSTYNFQGYKIYQLANAQVSPSEYGDVTKAKLIYQCDQKDGIDKIVNYDFDVLIGADV
                     PELMVAGNNTGIKHTFEVTDDAFAEGTTKLINFKTYYFSVVSYAYNDFIHYDPTDPNS
                     QKRPYLEGRKNIKVYTAIPHKPDPENGGMVLNAAYGDGPEITKIEGVGNGGNFQELTD
                     ASIQTILANSFMKDPTYKGRRAPVDVMVYDPVRVPAADFELRLVDSTNSNPLNPDSTW
                     WVLTNLTEGVSVTSDFPINIVNEQVLSDWGLAVTVHNVFDPGGQTVSGSFVEKEVNNG
                     FISATLSYTDPNKQWLTGVPDNDGTLLDWIRSGIADGTFVDVLPNPDENQVFEGVLGG
                     TWSPTELVAFSTAEVPYVPFRSITGLRPQIPIEETIGVDVVFTSDRSKWSRCIVVETG
                     ECERPTGEIKLDMRQQQSVNQDGVAEGSEVGYSWFPGYAIDVETGTRLNLFFGEDGCA
                     VQPDGRGKDMCFNPSDVFFDETFNPIYGGKHYMYVSKTQYDGCENIHDSLQWIGGSKP
                     ETSVYKDIFKNIVWVSMPFLNTGYTSAVGSPMLVPTETTIQLRVTKPYSNKFVTGENL
                     GFPLYQFSTANLAPSVNDTETASAALDLIRVVPNPYYAYSAYETSSLDNAVKITNLPS
                     KCTISIFTLDGTLVRRFERDVTSDNTAGGELNSKSSNLDTSLEWDLKNEKNVPIASGM
                     YIIHIDAGDLGETTVKWFGVMRPIDLDTF"
     gene            complement(35677..39393)
                     /locus_tag="PLL28_00880"
     CDS             complement(35677..39393)
                     /locus_tag="PLL28_00880"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNF67892.1"
                     /translation="MNKLLYALVVLVLCVGSAAAQSGEIQGKVTNDKSEGIPFANVVV
                     YKNGVLQTGATTDFDGKYSIPALEPGTYEVEASYVGLKQRLTGITVSQGIVFLPDIVL
                     SEQVLQTVVVKYQAPLVDKGNTSTGGVVTKEDIQKIATRNVTSIAATKEGVYQSDEGG
                     GLNIKGSRGDATEYIIDGVRVSGSLKLPQDAIEQLEVITGGVDPKYGDATGGFITITT
                     RGPAKDYNGSIELASSQYLDDYGYNLASLFLTGPFIVKNKGTDSAVAKFGFFLAGELE
                     MEKDPDPSATDVWAVKGDVMQSLIDDPLRPSVTGAGFNKNSEFITKDDLETIGARDNV
                     NNYGLSLNSKLDYKFSKNTNLQLGLSWRKFHANTYSRNFALFNAENNPVDDQNTYRGY
                     LRFTQRFPEKRSVDNKESVVGNAYYSIQLDYTKFQSSRQDADHGMNPFAYGYIGQFNT
                     YKAPVYFYTTDETTGLTGWVLAGYADTLVEFTPGEANPALAEYTTEYINGSALPPSSL
                     FDIQLGGGLLNGDASNLFLSTYNMWWNYGVPWFNYSKSDDDQYSLNFAAALDLKNPKS
                     KKVGKHSIEFGFEFQQRVERFYGVNPIGLWTIARQLTNKHILTLDTDNPILLIDGEQY
                     TYDQYLSNPDLYFGEFDTITYNRLSLANDQAYFDAHLRDKLNSMGYAVGDLDYINIDN
                     LPLDVFDLNLFSADELLNQGSSLVFYNGYDYLGNAQTGTVSFNDFFTQEDDNGVKTRP
                     IDAFRPVYTAGYIQDRFNFNDIVFRVGVRVDRYDANRKVLIDKYSIYDVYSTAEVDGS
                     LNPNGSHPGNIGQDYVVYVDDFNSPSPTILGYRDEDTWFNADGEEVTDPNVIAVTSST
                     GTITPYVVDPDADIKDANYDPNSSFEDYTPQITIMPRIAFSFPISKVEDREALFFAHY
                     DILTQRPPTGTTTTPADYYFFLENAGALLDNPDLKPEKTIDYQVGFQQQLSTSSVIKI
                     SAFYKELRDMIQVIQVPYAYPLSYTTYGNLDFGTVKGLSISYDIARRTRNLKLSTSYT
                     LQFADGTGSSATSQINLVGAGQPNLRTIIPLSYDSRHAIKLVLDYRFENPKGSQPDWL
                     DNAGMNVTFNARSGEPYTRQSNPLPTAQFGINNRSSLEGAINGSRLPWHYRTDIKVDR
                     AFEFGLGKGERPVSMDVYLWVQNLFDAQNVIAVYPYTGNPTDDGYLTSAEGIEAISSQ
                     VNPAAFVDLYNIKMDNPDNYSIPRRIRVGVSFDF"
     gene            complement(39517..40467)
                     /locus_tag="PLL28_00885"
     CDS             complement(39517..40467)
                     /locus_tag="PLL28_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNF67893.1"
                     /translation="MAKKRVIITDYLHSYLADWLHSQGYQVDVQPDITNEGLAEVIDN
                     YHGLALSTKIKVTPTLIDKATQLEFIARAGSGMENIDVAYAKSKGIHVVNSPEGNANA
                     VAEHALGLLLSVLNNISRADRDIRAGVWQREENRGFELMGRTVGIIGYGHTGSAFAQK
                     LRGMGVYVLAHDKYIAGFGDENVEEVSMEQIQLECDVISFHLPLNPETMHYCNAAFLA
                     KCRPGIWLINTSRGKIVNTSDLMEALDSGKVRGAGLDVFENEQFYTLAGKDKEQMEEL
                     CRRQNIVLTPHVAGWTVESYLKLSKILAEKLGALGLSATM"
     gene            complement(40469..41242)
                     /gene="rsmA"
                     /locus_tag="PLL28_00890"
     CDS             complement(40469..41242)
                     /gene="rsmA"
                     /locus_tag="PLL28_00890"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNF67894.1"
                     /translation="MGFQHNKSFGQHFLKQPETAGRIVEALSPLPEQIPILEIGPGLG
                     ILTKFLLQTEHPIYCSEIDHRIIAFLKEKLLMPSDHILEGDFLRLDIRKYFQQPLAVI
                     GNFPYNISSQILFVVLDVMELVPVVVGMFQREMAQRVTADHGNKDYGIISVLIQTWYD
                     TEYLFELPPDAFDPPPKVHSAVIKLRRKQVAPDVDPKALKNVVKTAFNQRRKKLSNAL
                     SGIPNAVPVLHQLNFAGKRAEELSVSEFLTLTKAIQAAK"
     gene            41347..42405
                     /gene="pdxA"
                     /locus_tag="PLL28_00895"
     CDS             41347..42405
                     /gene="pdxA"
                     /locus_tag="PLL28_00895"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNF67895.1"
                     /translation="MENRIRIGFTLGDVNGIGPEVVIKALEDKRMLGLCTPVIYGSNR
                     IISFYKKLFNLEGLSYTTINSADQANAKAVNIINCITQEVMIMPGQENETGARVALTS
                     LEAATNDLLKGQLDAIVTAPINKHNLQSIGFNYPGHTEYFNAKAPESESVMLLMQGDL
                     RVGLATGHIPVKDVASALTKEGLLRKLEIIHHSLKQDFASTGPRIAVLGLNPHAGDSG
                     TLGAEESNIIIPAIKAAQEKKMFVTGPFSADGFFGSGQYQHFDAILAMYHDQGLVGFK
                     SLSFGGGVNFTAGLPFVRTSPDHGTAFDIAGKNEASAESMLQAIFTAVDIVRSRKVYH
                     EITANPLKRTEVASERGA"
     gene            42574..43077
                     /locus_tag="PLL28_00900"
     CDS             42574..43077
                     /locus_tag="PLL28_00900"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNF67896.1"
                     /translation="MKQGVHHFNYELDDVFFGHFPESQVQQGKVFVDLALDKKERLFV
                     LNFDVSGSISTDCDRCGQPFDLPIHGNYTIYVKLGDKREEDVDSEEVVWIPDSESILD
                     ISEMVYEFVHLSVPMLKAHPDKAGGVPGCDPEILKLLGQEEEGSQDEKGPDPDPRWDI
                     LNNLNIN"
     gene            43089..43268
                     /gene="rpmF"
                     /locus_tag="PLL28_00905"
     CDS             43089..43268
                     /gene="rpmF"
                     /locus_tag="PLL28_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNF67897.1"
                     /translation="MPNPKRRHSKTRTRSRRTHHGVDMPNVSACANCGAPVLPHRVCP
                     ECGSYRGKIAIEKKA"
     gene            43307..44302
                     /locus_tag="PLL28_00910"
     CDS             43307..44302
                     /locus_tag="PLL28_00910"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNF67898.1"
                     /translation="MQKTLAAITGVYGYVPEYRLTNAELERMVDTTDEWILTRTGISE
                     RRILKGEGLATSDMVVEAVKGLLAKTGNTAGDIEMLIVATTTPDMQFPATANIACDKL
                     GCNHILSYDISAACSGFLFALSTAAQFIESGKYKKVVVVGADKMSSIINYTDRATCVI
                     FGDGAGAVMLEPSTDGNGIADAVLKSDGSGRHFLHQKAGGSLKPASHETVDNMEHTVY
                     QEGKTVFKFAVKNMADAAEEIMLRNNLKSDDISWLVPHQANLRIIDATAERMGLSKEK
                     VMINIQKYGNTTAGTIPLCLWEWEPQLKKGDNIVLAAFGGGFTWGATLVKWAYNG"
     gene            44410..44973
                     /gene="efp"
                     /locus_tag="PLL28_00915"
     CDS             44410..44973
                     /gene="efp"
                     /locus_tag="PLL28_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNF67899.1"
                     /translation="MASTADIRKGLCIDFNHDIWQIIDFQHVKPGKGNAFVRTKLRSL
                     TTARVLENTFPSGATINPVRVERRTYQYLYNDETGYHFMNNETFDQVTLDGEMVDGKE
                     FFKEGQEIDILFHADTEKPLFCELPAHIILAVTFAEPGVKGNTATNAMKYATVETGAR
                     IQVPLFVEEGDVIKIDTRTFEYVERVK"
     gene            44979..45473
                     /gene="accB"
                     /locus_tag="PLL28_00920"
     CDS             44979..45473
                     /gene="accB"
                     /locus_tag="PLL28_00920"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNF67900.1"
                     /translation="MDFKEIQELIRMVNKHNVAELTIENQDFRIKIKTALAATTMVQA
                     PMMMPQMQQPVMMPQMSAAPEKPQMAAAETKSDAAESTGGNTITIKSPMVGTFYRSPG
                     PDKEAFIKVGDEISVGSKICIIEAMKLFNEIESEVSGRLVKILADDASPVEYDQPLFL
                     IEPK"
     gene            45488..46876
                     /gene="accC"
                     /locus_tag="PLL28_00925"
     CDS             45488..46876
                     /gene="accC"
                     /locus_tag="PLL28_00925"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNF67901.1"
                     /translation="MTKKSSKPQELIRPIKKILIANRGEIALRIIRTAKEMGIKTVAV
                     YSTADKDSLPVRFADEAVCIGPPSSKESYLNIPKIMAAAEITNADAIHPGYGFLSENE
                     MFSAICAKYGIKFIGPMPEQIHQMGDKITAKETMKKAGVPVIPGSEGLLKDIADAKKQ
                     AKRIGYPVIMKATAGGGGKGMRIVWNESEVENAFNTASMEAKASFSNDGLYMEKYIEE
                     PRHIEIQVAGDRYGKACHLSERDCSIQRRHQKLVEESPSPFMDDKLRQKMGDAAVLAA
                     ESIAYEGVGTIEFLTDKHRNFYFMEMNTRIQVEHPVTEEVVDFDLIKEQIKIAMGEKI
                     SGKNYFPELHAMECRINAEDPSRNFAPSPGRITSFHSPKGHGVRVDTHVYAGYTIPQY
                     YDSMIAKLICKARTRQEVILKMQRALDEFIVEGIKTTIPFHKKLMRDERFIAGKFDTS
                     FMNSFDLSNLGD"
BASE COUNT        13879 a        10077 c         9281 g        13639 t
ORIGIN      
        1 tgatcaaccg gaatataaac tgcatcaatg gatgaatgcg caggtgagca ccgcattgaa
       61 agatggcgat gtattggtat ttatgaccga tccgaaagat gaactccatg ccgatcatcc
      121 gcttattcaa accctacaac aaattcgcac accggtaatt gtagtcatta ataaaagcga
      181 catcattgca aaaaatgtgc tggagcaatt aatgattaaa tggacttctg tgctgccaaa
      241 agcaaccatt atttccattt cagcattact aaaaattaat accgacaaat tatttcaaca
      301 tatagtagat gcattgccgg tgcatccacc ttattacgac aacgacacct ggaccgacca
      361 gaccgaacgt gtgattgctg cagagatagt tcgtgaaaaa atattcatgc aatatcagca
      421 ggaaataccg tataatgtag aagtgatagt tgacagttat aaagacaaag gtgatattat
      481 tgccattagt gctgtaatct atgtcggacg tgatagtcac aaatccatca ttattggaaa
      541 atccggatca gcaataaaaa atattggcat agctgcgaga caggatattg aaaaatttgc
      601 aggcaaaaaa atatttcttg aattgtttgt aaaagttcgt gagaactgga aaaatgacga
      661 tagagggcta aaacatttcg gctacgaata aaaaaaatgc ccgctcaaaa taagcaggca
      721 tttataatga ttgaattatt gaatatttat tgttccacca atttaatttc cacgcgtcgg
      781 ttttgcgcac gtccggctga agttttatta tcggcaatag gttttgtctc gccaaatccg
      841 gcagaactca atctcgcatc ggcaacacct ttgctgccaa aataattttt cacagccgct
      901 gcacgcttgt cgctcagttc ctgattgaat ttatcatcac cctgatcatc agtatgtcct
      961 tcgatgctcc agtttgttcc gggatataaa atcataatag caactatctg atccaaaaca
     1021 ggataggatg attttttgat tacatcactt ccggattcaa attgaattga ttttgctgcg
     1081 aagttgattt tttcaatgat cgcttttttc gtttcttcct caataacagg acaacctccg
     1141 gtagctttta ttcccggttt atccggacat ttatcatcta tatccgctac gccatcgcca
     1201 tcggaatcag gacatccttt caatgcaatt tctccacgtt cgcgaggaca tctgtcttct
     1261 ttatccatga tgccatcacc atcagtatca ggacatccgc caaatttcgc aaggcctttt
     1321 tcctgcttac aggcatcatc aagatcagca atgccatcgc catcagaatc ggggcatcca
     1381 ttgaattctt tcaggccttt gtcttttgga caacggtcgt ctttatccgg aatatcatca
     1441 ttatcactgt cagggcaacc gctgaattta gctaatcctt tttctgtagg acaggcatca
     1501 tctttatcag ccaatccatc gctatcggaa tcggggcaac cattgaattg caataatcca
     1561 ttttctgttg gacaggcatc catcgcatca gtgatgccat cactatcggt atccgggcat
     1621 ccgttgaaaa gcggaatacc tgctttatcc ggacaactat cttcccaatt tgcgatgcca
     1681 tcaccatctt tatcttcacc ttttccaaag cgaattacca aaccgactgc atgttgcata
     1741 taatcagaac ctgtttcact gaattgatgc gtatagcctg tttgtaaatt gataccaaac
     1801 atgggagtga gccataaatt tgtgccgatg gcaatttcgc cttcgggaaa taatgtagtg
     1861 gcatcaggat catcagaatc gagatagtta gcacctacac cgccggcaag atagggcgca
     1921 aaccaactat cgtgcggaag catgtatccg tttgcaaatt gataccgcaa tcccaaatca
     1981 acaccacacc ataaaaatgt ttcgcgaata tttcctttag agatggttga aatcgatgca
     2041 gctgccaacg atgcttccat agaaaaagaa ttattgatgg aaccgccaaa cgtgtatcga
     2101 tttgctaccc atgcaccatt gatgtcggca ctatcgaaaa ggtctttcgt ataaccgtca
     2161 tcttcaatat tgtaatcaat aaaatgtgca ccaaatccaa aggcataagg atggtctaca
     2221 ttttgcgctg agatgctgcc aattaacagt cccaacgtca ttaagggcaa gtaacgaagc
     2281 ttcatagact gggttttaag gtcaagaaac ccctaattaa caaatatgca ttgtaaacac
     2341 atagaatatt aaccacaact tatgcacctt ttcacttttc ccggtgttta gtaaacctga
     2401 gaaatagcgc cttgttgctt acctttacac cctatttatc aatgcacatg gaaagagtaa
     2461 aatgcctcat tttaggatca ggaccagcag gatatacagc tgcaatttat gcttcacgag
     2521 ctaatcttca acctgtagtt tatacaggca tcgaacccgg aggtcagtta atgcaaacaa
     2581 cggatgttga gaattttccc ggttacccgc agggaatcat gggtcctgaa ttaatggagg
     2641 acttaaaaaa acaagctgaa agatttggca ctgatgtgcg atttggcatt gctacaaggg
     2701 ttgaattaaa cgaccgtgta aaaacggttc attttgatga cggcacatct attgaagcat
     2761 ataccgtaat tatttctacc ggtgcctccg caaaatggct gggtttacct tcagaaaaac
     2821 gtttgaacgg aaatggcgtg agtgcctgtg cagtgtgtga tggcttcttt ttccgcaatc
     2881 aggaagttgc attggttggt ggaggcgata ctgcatgcga ggaggctctc tacttatcta
     2941 aattatgtac taaagtacat atgcttgttc gcaaggatag tttcagagca agtaaaatca
     3001 tgcaggaacg cgtactgaat gctccaaata ttacggtgca ttggcattca gaaacgctcg
     3061 aagtattagg cgatcaaact gttaccggaa ttcgcattcg caacaacaaa acgaatgaag
     3121 aaacggttat caatatcacc ggtttttttg ttgccattgg tcatcagcca aatacacaaa
     3181 tatttaaaga tcagattgat cttgatgaac aggaatatat cataacaaaa gccggcacca
     3241 ccaaaacaaa tattgaaggg gtttttgccg caggcgatgt acaagataaa cactatcgtc
     3301 aggctataac tgctgccgga acgggctgta tggctgcatt agaagccgag cgatatctga
     3361 ctgaaaaagg aatacattaa taaatcgtca ctttttggtg atgaactttt cactccattt
     3421 gctgttcagt taagcatatg agaagtaaca cctttccttc tatacatgcc ctgaatgtaa
     3481 ccgcattatc cggtcgtact ttttctatga gtgagtatac cggcaaggtg ctgctgatcg
     3541 taaatactgc cagtaaatgt ggtcatacct atcaactcgg attattggaa gaattgtatc
     3601 aggaatacaa agaccgtgga ttggtagtaa taggtttccc atcagatcaa tttgcacaag
     3661 agccattaaa cggtgagcag atacaagaac attgtcaggt gaattatggt gttacctttc
     3721 cgatgatggc aaaatcaaag gtccgtggcg atgaggcgaa tgctgttttt aaattctttt
     3781 cgagtaaatc gctgaatgga aaattcagta aacaaccgcg ttggaatttc tataaatttc
     3841 ttgttggcag gaatggtgaa gcactggatc atttctggac ttatcgcaaa ccgaataacg
     3901 ctgcgctccg caaagcgata gaaagagcct tgatggctac aaatcctaag taaactcggt
     3961 tatttttgtc tgatgaaact ggatattctt gcctttggtg cgcatcctga cgacgttgaa
     4021 ttaggatgta gcggagtact gataaaggaa gtgcgcaacg ggaaaaaagt aggcatcata
     4081 gaccttacac gtggcgaact gggatctagg ggcacagcag agatccgcgc acaagaagct
     4141 gccgatgctg ctcagatcat tggttgttct attcgtgaaa atctggaact tccggatggt
     4201 ttctttgagg ataataatgc taatagggct attgttattc aggcgataag aaaatatcaa
     4261 cccgatatta ttatctgcaa tgccattcac gatcgtcatc ctgatcatgg cagaggctcc
     4321 aacttattag agtcctccgc atttttatcc ggattaatta aaatcgaaac aaccgaaaat
     4381 aatatcgcac aaaaaccctg gcgtccgcga ttggtattgc attatctgca ggatagatgg
     4441 ataacaccgg atgtaattgt agatatttcg gatgtatggg aagagcgtat ggaaagcatc
     4501 gctgcacacc gttctcaatt tttcgatccg ggaagtaatg aaccggaaac gtatatctct
     4561 tcaaaaaatt tcttcgaagc gattgaaagt cgcgcacgtg aaatcgggag gccggcacaa
     4621 ttcaaatatg ctgaaggctt tacttgcagc cgcaccattg ctgtgaagtc gctcggcgaa
     4681 ttgatgtgat tatttttcag tgttagtttg ctctaaaaca aaaaaccctg atcgttagac
     4741 cagggttatt caaactatat attcatgaca gtcctttatt ctgcgacaga agttgatttt
     4801 acttctttga tacgagctga ttttcctttc agtccgcgga gatagaatat cttagcacga
     4861 cgtactttac ctgatttatt tacctcgatg ctttcgatgt ttggtgaaaa taaagggaat
     4921 accctttcaa caccaacgcc attagaaatt tttctaacag tgaatgtttg cgtacgaccg
     4981 gtgcctttgc gctgaatcac atcgcctttg aaagcctgtg ttcttgactt atcaccttca
     5041 atgattttat agttaacagt aacagtatca cctgctttaa actgcgggat ttcctttgat
     5101 gggataaggt cttgtgcgag tgattgtaag aattccatac actaattttt aaagtggact
     5161 gcaaagataa caggttttag tgtttttgcc aactttccgg cttagttttc cggaaggagg
     5221 tccggacggc gggatttggt ctttttcaga gcctcatcat gtctccattc ctcgattttg
     5281 gccgtatgtc cgcttagcag gatttccggt atctcccaac cctgaaagtt tgccggacgg
     5341 gtatacaccg gaggagccaa aaggtcgtcc tgaaagctat caaacaaagc agaggtttca
     5401 tcacccaaaa cacccggaat cagcctgcca attgcatcta caagtatcgc cgcagccagt
     5461 tcacctccgc tgagtacata gtcgccgata gaaatctctc tggtaatcag gtgctcgcga
     5521 atgcgttcat ccaccccctt ataatgtccg caaagcagaa tgatattgcc tttcagcgac
     5581 agctgattgg ctatcttttg gttaaatcgc tctccgtcag gcgttagata aatcacctca
     5641 tcatagatac gttgggcttt caaggcgttg atgcaattga agatgggctc caccatcatc
     5701 accataccgg caccaccacc aaattgataa tcgtcgactt gcttatgctg tccaaggccg
     5761 taatcgcgca actgcaccag gttaacttct agcaaaccct tgtcggcagc acgcttcatg
     5821 atactatgtt gaaacggaga ctccagcaat tcgggtaata ccgtaataat gtcgatgcgc
     5881 atagggttat gaattaaagg tccgtaagtc cctccggaag cttagtaaac aaggtttcag
     5941 aggattcatc ttctcgaata atgaggtggt catgcatggg caccaacagt tcacgaccct
     6001 gatggctaac tgttagcaat agatacgccc ccatatcggc agaggcaatg atttgaccaa
     6061 gttcgccata ggtttcatca tgaattttcc atccttcaaa ggctatttcc tggtcttcat
     6121 cagtatcttc aagccactct gcaggaccaa aaactggacg ttttgtgtag agatctgctt
     6181 cttccttatt gtgcaattct tcgagcaata caagggcatc gccattatta aaatggctga
     6241 tctcgctgat gaagtgaggc atatgcactc ctttaattaa aaagtaaaga gcttccgaaa
     6301 ccgaaagctc tttacaatta tcatcaaatg ctatacgcac atgtcccttt acaccatgcg
     6361 ttctgatcac ataaccgatt tcgaccagtt cgccttgcac gggatttgat tattcgttag
     6421 ttgtctcctc agtagtagca tcattagctg cttctgaagt tgtttcttcc accgcagcct
     6481 cttcagttgc agtttctacg gtagcagcag cttctgcttc gcgttcagcg gccgcttttt
     6541 tagaagcttc ttccaaacgt tttgcattga tacgtgctac ttcagcagct tttgttgcag
     6601 cagcagatgc ctcgatagct ttaaccttat cgataacagc tgtttcgtgg ctttttaccc
     6661 actcatcaaa tttctgatct gctacttcag tagtgaaggc accttttgcc acaccacgaa
     6721 ggaggtgttt tttgtacaat acccctttgt agcgaaggat agcattaaca gtagttgtag
     6781 gttgcgcacc tttttgcagc cagtcaacag ccttgtctac atcaataatg atttgagcag
     6841 gaactgttgt cgggttataa gaacccaggc gttcgatgaa tttaccatcg cgtggagccc
     6901 ttacgtcggc taccacaatg tgatagaaag gagctttctt tctaccgtga cgttgtaatc
     6961 tgattttagt tgccatgtta aaacatgctt tttagttaaa aaatgtctca gatcgacccg
     7021 gaccagccgg agagttgaga ttggggtgca aagatacctt aatcgggtgg tttttacaag
     7081 tgaaatgcct attttcgccc tatggcgcag gaaaactcag agcgtgattt gtcggcaagg
     7141 gctgttaaaa tccttcgctg gcatgagcac ctgatctcct ttttccagca gtatttaaac
     7201 gagaagcagt ttgtcaccat ttccggggta ttaataggca tcacctctgc cttggctgca
     7261 attttgctta aggccatggt gttctacatt catgccctcc tgttcacaga aagcaatatc
     7321 ggtaagatct caccttatat ctatagttca tttcccttat taggtatatt agttacaact
     7381 tggatagttc agcggtattt tggcgggaac ctgggtggtg ggaatgcggc cattattttt
     7441 tctatcaaaa agaaggaaag ccgcctgccg ggaaaattaa tgtactcaca tttaattacc
     7501 agcgccatta ctgttgggac tggtggctct acagggttag agacaccaat tgtgactaca
     7561 ggtgctgcca taggatccaa ttatggcaaa acatataaag taggaccgga ggaaaggacc
     7621 ttaatgattg cttcaggtgt tgcagccgga attgcggcaa cgtttaatgc accgattgct
     7681 ggtgtgttgt ttgcagttga agtactgata agtgaaatca ccatagtagc atttatccct
     7741 attctcattg ctgcagcttc cggtgccatt ctgtcgaaga tcattttaaa tgaggaaata
     7801 ctcctgaact tttctaacat ggcacctttc aattatcaca atgtgccgtt ttatatatta
     7861 ttaggattgt tatcgggagt aattgcagtt tactatatca gaacctcgct gcgtatagaa
     7921 agaatatttc gcagagttaa acaacggttc tatgttcaac gagcaatcat cggcggaata
     7981 ttattgggta tattgatatt attatttcca ccattattcg gcgaaggcta tgagagcatc
     8041 aaaactttag caggcaatca tgcggaagac atattaaaag gttcagtagt tgttggcaac
     8101 ctgcaggatc ctgtgatgat gattattgta ctcactttcg tattattact taaagtggtg
     8161 gccacttcca ttacattagg aagcggtggc aatggcggca attttgctcc ttcattattc
     8221 accggtgcct atttgggata tatttttgcg ggactgataa atcttacggg aattttcaac
     8281 ttgcctccgg gcaactttac agttgttgca atggcaggaa tgctcagtgg cattttccat
     8341 gcgccactca ctgcaatatt ccttattgct gaaataacag gaggctatga cttgatggtg
     8401 cctttgatga tagttagtgc tatcagcttt ggagtttcaa ggtatttgtt tacttatgca
     8461 cttgatggtc taaaattaaa tagtatgctt caacaggctg caagagagga acaagaagaa
     8521 aataaagcta aaaccgaata attatttcaa ccaattactt agatctttgt tcagcagcaa
     8581 ctgcaattgc tcaatatcct tcctgtaata atttattaga tattcctttt cttcggcagt
     8641 aatagtagga atattttctt tggtataaat aagcgattta agtctttctt ttaaattgga
     8701 aggaaatatt ctcttcacta aaccatacac accaatttgt gtaagccaat aatttagttt
     8761 accaaacctg ggcattcccg cctcattata tctttttgac atgtcgggtg tgaatgttgc
     8821 atctacacct aaaaacttaa acagcgattg catagtgcct gtaggatctt tcttgtaatc
     8881 gtcgtaatac aatatgcaaa ctttattctt gtcaaaaata tctaaatagc gttttacctg
     8941 ctcataatac atgcccacct ccagatataa atgacttaca ccccatccct tcacaggttt
     9001 tacctggtct gcttcaattt ccttcagcaa atccttttcc actatttttc caagtttcag
     9061 attcatgaga aattgtgaat acaatcgctc aacaggatta cgtaatatca tcacaatttg
     9121 cacatccggc aaagctttgt gaattgccgc agctgtagaa ggcaaataca aataagaatt
     9181 actcacctct cctattgctt tttgagaggt gacattttta aacaaagcag catattgctc
     9241 tgcagttctg acatgcgcaa tatggatttt cttatccata gggccggcca gatatttttc
     9301 cagattcgca ttgatatcct gcttgtaatc aacattgaag tgatcaaaca gcatatcggc
     9361 atcactgaaa tgatttgtct ccttaaccgg cgacatatac acttccggat gcatttgcag
     9421 gtattcatgg atagaagtag tacctgcctt tacagcgcca acaataagga atgtaggttt
     9481 cgctgccatt tgatattaac gtaagaattt ccagaatatg attctcgcta cgttcacaat
     9541 tctgtatagg aaaaaggaat gccatggaat acgaatatga ttccaactct caggaaagtc
     9601 gtagccccat cgttgcatat atgggccgaa aatatattta gcacgacgct tagctttatc
     9661 actttcgtaa tattcccaaa atgttttcga cttctcggca gttttattgg caacaggaat
     9721 atcacgtact ttgtctacac ctatcaattc aagtgctttt gcaaaatctg cttgtaagtt
     9781 ctcgaacctg atgacataat ccatgcgttt atgttcaatg atactccaac tggcataagg
     9841 taaagtatag gcacgcatga agaaattctc aaaattctct ccaccctcaa tgatagattt
     9901 atactgacgt acattccacc accgcataat atacttcctc agaaaaccgt gcttcatcag
     9961 gcgaggattg gaatatctgt tttcatgatc tgtcttgaac ttaaaataat tgctcacaca
    10021 aatatccatt gggttcctca acccactgac gcgcgtaaac tgcttctctt caggttttgc
    10081 ctgcttcaaa aaatctgtgc tatacagtgc atgtttgaat aatatttcat gcccatcata
    10141 attttccttc aactcacggg caatagcact gcttgcagtt tgagggagtt cgatgaaaac
    10201 gtacttatac ttgtgactaa tgatcatgat tcaaactttg tgtttaagat agcttgataa
    10261 ataacttttg cctcgctgaa gtttcctttg cttacatatt taaaacttct tgtgataaga
    10321 tgcaagtata aaaagggcaa ttgcgggcga taatacttct tatataattt aattttccct
    10381 cgatacttat agtgcagcgc tttaatctgc cacttacttt tcttagggct atttcctgta
    10441 gatgcacctt gtttatgata caccttgctt tttaccgcaa caccaacttc ccagtctttc
    10501 aatttagcac gtgcgctcca gtcattttcc tcgttataaa gaaaatactc ctcgctcatc
    10561 aatccaacat ccaataaaaa cggtctactc acaaacattg cagcacctat cacataattg
    10621 caacttattt cctgctcatc atattgattc ttatcaattt cctgtgcacc tatgatctta
    10681 ctctttccgg aatatttatt aaatattgcc cctataccct gcattttctg tggctggtga
    10741 tacaccatga gcttgctgcc aacaataccg atagtttgct ttttctcttt ttttacctcg
    10801 caatacttca ccaactcttc aattgcattt ggttctataa cggtatcatt attcagcaac
    10861 cacacataat caaactcgct attaagcgca taccgcatac caacattatt accgcctgca
    10921 aaacctctgt tttcatcact tttaataaac aacaatggca tgatgccatc cagtggtcct
    10981 aaacgcatgt gcgcagcctc ttcaggtgaa gctctaaagt acagcagtgg tttatgaacg
    11041 gggggatgtg tgagatgaaa aagggggttt tgagtggtaa tatcaatagc attttcaccc
    11101 cgagcccatt tcaacatttg ttcccagcta tcatcgccgc taccattttc tactacaacg
    11161 atttgataat ttgtgtagtt actgcgcagt acactctcca tacattcgag tgtatctttc
    11221 cacgtcttat aatttaccag aacaacacaa actttaggtc gcatagggat attattttaa
    11281 ccaacctgaa aggtctttat tcaacaagga ttcaagtgca tgtacatcag gtccaagctt
    11341 ctctaacaaa aaagattttg ctgccggtgg catttgaccc ttatcagctt tctgtttata
    11401 gaataatttc acaactgcac cctttaggaa tttaggtgag atgcgcttca taaatggtct
    11461 gatacctgat ttattcagga accaggtcag attcttatac tttggcgtat acacctcatt
    11521 atattgggtt gaagtatccg gattaaaaga tggatcaaca ccaataaatg tgtagaggtc
    11581 acgtattacc tgagcactat tttttttgag gtcttcagta aatatcactt taacattctc
    11641 ggcaccaaaa gtatccaggt agcgttttac ttgctgataa tacatgccga tttcagtata
    11701 taaatgggca tttccccata ctttgggctg gtgctgataa tcttcaagca atgccttatg
    11761 gaaatcaaat tcattgctat tgcccgtcca taaattcatc ctgtaatgag aatatgcgcg
    11821 ttcaattgga ttgcgcaagc aaataatgat tttacagttt ggaatcagct ttttgatctc
    11881 tgttgcggca actgttgaat acaaatagga agtacttaat tcgccaattg ccttttgcga
    11941 tgtaacattt ttgaatagct tcaaatattg ttcttcactt cgcacaaatg cagcatggaa
    12001 ttcctgcttc atatcaccct gcacgtattt ttcaatatcc tgaagtttat tatgtgcaaa
    12061 ttcctttttg aattggttta catcaatatc cttactaaag tagtgtggtt cctttatcgg
    12121 cgacaaataa acttcaggat gctgcttcag gtaatgatat attgatgttg tgccgccttt
    12181 atttgcgcca actactaaaa atgtgggtaa tatggttttg ccggtggtca tgaattattt
    12241 caactgcaac aatttatcgg caacttcctt cgataggcaa tatacattat tgatctgctc
    12301 gatgttcgga attttgatag accctgcgag tttataatca ttaatattaa aaacctgaac
    12361 catattcggg acagcttcac cgccggggaa aagtccgaga tatcgtttac gggtttcact
    12421 tacgcctgca taatacacat tacgttcaac atccagatca ccgccgcggc caaaaccctt
    12481 ggtatccata gtgcgaagga aattccagtt cttatcaaat actttcaatt caaaatgtcc
    12541 gctgtcgaac acccaatatt gattattgat tcttcttaca ctgtggggag ctttgaaatt
    12601 taatggatag cgtttttttt cggtgatatc taccactccg ccatttccct gacttttaat
    12661 aaatttttgc gctattcgct tcatcaattg acggccggat gtatgatgaa cgagaatata
    12721 taaatcgcct tcgtatcctt caaatatcaa attacaatga aatttatcaa ccacttccac
    12781 atctgcctca ccttcaccct cttccggtgt atttgattta aagtcggaaa ggttaattcg
    12841 ttggtctacc ctatttgtgc ggatgttata gctaacgatt tcatcatgct tagtgtttga
    12901 tatccaaact tgctctccta tcattgaaat ctcatgtatg tcagtgaggt ttggaatatc
    12961 caattgctca tatctgtttt ccttgagcat gaatttaaat acttcgctgc tggaagcgag
    13021 ataaaaaaca aatggatcgt ttggatggaa gcacatactg ctcataccta attcacccca
    13081 agtattaaag aaattcacag gatgtatctt tccattttca tcgattactt gcatgctacg
    13141 gatatcctgg cgactgatag ctctatcagc aggatttgca tttaatgaac cgataaccaa
    13201 aattcttttg tcgggatgaa taagttgcat aatctaaatt tagaaagaat tttttaaaac
    13261 tgcagtttca ggaaggcctt gcctgtccat ttgaacttct aataatttat aagcataaat
    13321 cacaagaata aaaattgctg ttgagccggg ctgcataaat aagtatcctt ttgcaaggga
    13381 tgataaaaaa caaaatatca aaaagtattt cgatgcaatc atgaatggct catctttata
    13441 acggcgaata aattttacac ttctgaatgc tattaaaaat tgaagataaa tcaaaacact
    13501 tcctaagata ccatacgtca taatagaccc gagtatacct atgtctcccg gaggcacagt
    13561 agctattgta tgctcgcccg caactgccga ctcggcactt agcttaccaa tgcctatcca
    13621 ccaaccgatt ggatagcttt gcaaataaac aaacaagatt gccatctctt ccaaacgcgc
    13681 atcaacagag ctttcgcccg aagcattccc tgtgaagaca actgtagcaa gagaagtata
    13741 ttgaattaca taatcgttga tgcgatcagg aattgcagca tataaaatac ccattattac
    13801 aaaaaagcca ataccaatag aagtaaatat tctaaaggcg cgctgccagt tcacttcaaa
    13861 catataaaat acaaataaag tggccaccac cgaaatcata accgatcggc cttggtggag
    13921 aaaaaacagg tatgccaggt tagcggctga taaccacatg ctgacagaat ccttttgttt
    13981 gatatacttt accgtatagt aaactgataa aaatgcgata aagtctatac caaatttaaa
    14041 tcgatagcca cctttgatat ccgagaaccc tacgaaagaa taatctaagt attgtttggg
    14101 atcaagaaat atataacacc aaatgaaata tgccatagtt ccccaaccca gccacttcat
    14161 cgctctaaaa aggtctccaa ttgtaaagaa ctttgtttgg agaagataaa tcaatacaaa
    14221 tatccctgaa atcccatacc aatgtcgctg cgagctaatg ccacccatca cattctgacc
    14281 ttcactcaaa ttggccatga aacccgccca caagggcatg ataataatta atatataata
    14341 ataatgtatt tcatccacgg tctttcgaac caggaaaata tacatcgcat aaataaatgt
    14401 gaacgtaata atgaaggtat accacaaaat ctcgaatggc atatagtaat ccactaaagg
    14461 cgttctggta aggatacgcg tggtaaaaag gataaatacc accaatatca tagtcttacc
    14521 aaagactgac attggttcct tataaattcc tttttcgtat gttaatatac ccatattaat
    14581 tataccacag cgacagatcg cgacctgtca atttttctgt gagatgaata tcttgaataa
    14641 aataatcggc aatcaattgt ctcctctgct cctcactcag tttaggagaa ctaacttttt
    14701 gcatattttg actgataaat gcctttctca tccattttct tatggcttta aaaggcaaaa
    14761 ttgtctttaa tatacgtttt gcaaaattag gctttctaac taactcattc aaggaatcat
    14821 tgataggttc acctgcctca ttgctatgca aatcgacatc cagcgccttg aagggaaccc
    14881 ctaaaaaggc ctgaaccttc tcaactgtcc cttgcctgtt tttgataaaa tcttcctcaa
    14941 ataccagaaa caaaaaattc tcagccggaa acacctttaa aaattgcgct atctgagtac
    15001 catacatgcc ccgttcaatg taagcaaaga tttttctggt cttcaaatct tccttcctgc
    15061 tcgcttcagc ctgaagagcg gcctcaaagt catgaggttc aagacccttt ctttttgtca
    15121 tcaggtagtg agaatatgcc ctatcggcag gatttcgcaa taccaccaat atcttaatat
    15181 ccgttcccaa cgtttcagca atgcgttcgg cagtatatgg aaacaacaaa taatcaggat
    15241 caaattcacc tacagcagat tgagttctgt agttgctgaa atattgatca tagaaagact
    15301 tacccttctg atagtcctga tcaaacaaaa aaaacttcgt ctccttaatg ggagggatgc
    15361 aaatatcggg atgcgcactt aaaatgtcgg acaaggtagt tgtgccggct ttttgtgccc
    15421 caatgcaaat aaaattttgt ttgatcccac tcatattata ctcttaacca atcactaagg
    15481 tccttctgaa caagtgtctg caattgcttg atatcctcag aatacaatgc aattaacttc
    15541 ctcctggtct cagggttcat ttctgttttc ttcaaattat atctcaacaa tttatcctta
    15601 gcccaatttc ttaatttctt ttttgtatcg cgactcatta actttctaaa gaaattaagt
    15661 atcggatttt tcctcgtcgt catcatttgc ttcaaccact taactttcat ttcaccactc
    15721 tggttgcgtt ttaccatttt cgcttcttgc aattgtgtaa cgccaagaaa ctgataaata
    15781 tcggacaaca acttttccgg ttgcttttca agcgtatcgt aaaacacgat tttaacatgc
    15841 ttgaaattgt ctatataatc ttttacttgc tgataataat accccttgtc aacgtagaaa
    15901 aaatcaaaat gccagttatc agccatacgt tgcttctcag catctattgc ctcttcaaat
    15961 gtcagcggct ccctcaagtc gcggcgatta tgcatatatt gcgaatatgc tctctcagaa
    16021 ggatcgcgta acacaatgag tattttcatc tcatctgcat tcgcaaccaa tcgcttgata
    16081 ttggcaatag atttttgatg aaaatataaa tatggtgttg aagattctcc catgacggcc
    16141 tcattatgat gctcaaacat ggcaagatat tcagcaggat tagtgaggta ccgatgacgt
    16201 gcttccttaa attcaggctg ctcatctaca taagtaaaga aacatggttc cttctgcaat
    16261 ggcatgaaga tatcaggatg attgcctaaa aattcatgca atgatgttgt tcctgctctt
    16321 gcagtaccta caatgagaaa attgggccaa cgttgcgtca tatttcctcc tcgttttttg
    16381 actcgttgat cacaggttgc attttctttt tggaaaatac actccataaa tcagcgaatg
    16441 gattataaat agtactaatg cccattgtaa aatatatctg cagtgcgcat cctacattca
    16501 atactgttct cgcaacagca gtagcaattg cagcacctat aataccgtat atagggatga
    16561 ggattaagtt caatgataca tcaattacca cagttgccaa tgcataatac atcgtaacat
    16621 tttggcgtcc ggtcatattc aacagaacag tcaccggacc tgtaagaata tttgcagctt
    16681 gtcccagtga aacgataaca aaaacggtta cagcttcact gcccgtgaaa tcttttccaa
    16741 aggtccacat gctgaatttc gggaaaatca aaaatatcgc cagtatgggt atacaggtcc
    16801 aaaaaatcat gcgcgttgcc tgcttaacgt tatttcttag actgcgttga tcgtttttac
    16861 caaaggcttc agcaaatttt ggtccggcga ttgaatttat agaaaccaat ggaatagtag
    16921 ataaggaagt caatcgtgta gcaacacgat aaatcgatac cattgcttcc ggggcatacc
    16981 atgccaatat taaaatatct aaccatgtat agatcatggt aagatattta gccatcatca
    17041 ttggtgccga caatttgagc atctcgcctg tatttattgg ctgctcttgc tgcgcgacgg
    17101 ctaatactgc cgctgcagca ttgtcttttg ctggtttctt ttcttttttg tctttctttc
    17161 tgccaaattc cgatccgccc acctccggat gcttcaaata atatatccaa taatattggg
    17221 caatggcaaa ggctacaaat acaccgaggc aataagataa attcacccaa agttcatcct
    17281 tggaaaaaag gccaaaaagc aataaaaatg cgatggctgc aacctggttg attgtttgag
    17341 aaatccaact atactgacgc gttttcttca gccctttaat acattcttca ctcaaaccgc
    17401 cgagcacata aaataaaatg gtgacagcaa atatttgcat gaagaaagta tactgatcat
    17461 aattaggaga acttgtatca aacagattat ccgtaaaata aggtacaatg aaaaatgtag
    17521 ctgccgataa aaacaatcct aaaaaaaata caattcgaaa tgcaacacta taagtatgtc
    17581 tgatcaaatc ccaacgccct gacccgccat actgtgctac aaagcgcgac atagccatat
    17641 ctaccccaaa tcttccgaaa attccaacaa tgctaaggat gctcagacca acagtataaa
    17701 cgcccatagc ttcctgacca tacacccagc caattaccat caaaagtaaa taacctgaaa
    17761 atctaccaac aagacttatg gcagtgcctg aaataccttt actaaagatc tccttaaaat
    17821 cctgatcctt gagcttacga cggaatttct ttgttatttt tctgatcata acgcatcgcc
    17881 ggtaccggaa ttggtttgtt gaagagaatt aagcggtgca acaggtcttg gagctatttt
    17941 accttgcatg atcaagcgat caatccattg atcagcgaaa atatccgtaa atagtttagc
    18001 gccgctagaa tttaagtgcg ccaaatcaaa taagttctct cgctcattga acatgggata
    18061 tgcagatgga ttttgaaatc caattctatt tttttcagga atgcgattat acaaaggaat
    18121 caaatattca tagcggtcgc caaggcgttg agaaagcatg atcactacgc tgatatcctg
    18181 cttttcacaa tctttgatga tcttcaacaa atccgcttcc atggttttgt taggattctt
    18241 ctttgcatct ttgaatttat catacagcat ctgacagcgc tgtgtgcgga agtcaatagt
    18301 gtcttgtgtc ctcaaatcat tatatcgaat tgccagatca ccttcaggat catcatgcat
    18361 ttcctgatca agagaataga agccgttttt atcaggacca acagataaag gctcaatatt
    18421 caaaccggca tgctgcataa taaccgaggg tcccatacca accaaaaaag atttttcacc
    18481 aaagctgaaa gcatgtaaga atggcacccc gaatttattt gctgtgccct tttcagactc
    18541 atatgaggcc ttcatcacat caccgatatt cccccagttg taccagtaca acatctcctt
    18601 tttatgcaag ttatcggcac ccatctttgt caattccgac aagtcgatca caacatattt
    18661 tatatgatcg gtattgcctt ccttcaacaa attttcaagc atatacatac tttgaggcgt
    18721 gcgatttgca ggtataccga agttgaaaga ttttatatgt aaatcaggat actgcgcatt
    18781 cactgcagca tcaaattgtt ttggatcaat gtgacggtat gtagtactag ctcctattaa
    18841 taatgtattg tattgttcag gattctcaga atatgtagta tacttcgtat tcaaacgcgt
    18901 gttaccccat gaaaaaggca tactcatcag tataagctgg cagatcgcga aaacgattac
    18961 caacaacaat ccgattctga gaattagttt tttcatgttt tagaatgcga aatagataaa
    19021 tgctgattta gtggtaactg caaaacatgc tacgattacc aaaaatatta cataaaaact
    19081 ccagcgcgta taccaagggc gaatagccac ctgatcttca atggtcgcac ttttacgaat
    19141 atacaaatta taatcgatga ataacagcat agcaataaat accagactga tccaaaattc
    19201 aattacacta ggaactgctc cttttggtgc actgttataa ataaattcat gcatggtact
    19261 tgccttgaac atctggccga taatatgcca cgcatcactt atagaattcg atctaaaaaa
    19321 tatatccgga atacaggtta gattaaaaat ccagatcatc aacaaaacat ttaccagaat
    19381 tggcatattc ggtatccact ttgccagtac catttttggc atatatttat ctctaaccct
    19441 acttactaca atataaaaac catggataaa accgaacatt acatatgtca ggttagcccc
    19501 atgccagaaa cccatcacgg ttaatgtaaa ccagttattg aataacattc ttccaaagga
    19561 gactctgctt cctcctaaag gaatatacag ataatctcgc acccagaccg ttaatgtaat
    19621 atgccatcgc tcccagaatt ctcgtatcga tttcgagaag tatggcgttc ggaagttgtc
    19681 ggtcagatta tgccccatga ttttcgcaga tcctatagca atatcgcaat acccactgaa
    19741 gtcgcaatac acctgaatag caaataacca agttgcgata actaccggcc atccgccatg
    19801 ttcaaacgaa ggataattcc aacctcccat tccattcgac tgaccataca ccgcctgaac
    19861 aaatattccc aatctgtcgg caatgacaac tttcttgaaa aatccgtata aaatcctggt
    19921 gagacctttt ttaacgcggt cgtaatcgaa atcatgatta cttttcaatt ggggctgtaa
    19981 atctcccgga cgttcaatag ggccggcaac aagctgaggc caataagaaa taaataaagc
    20041 aaaatatcca aagtgtttct cagcttttac atcgccgcga taaacatcaa tggtataact
    20101 cagtgcctga aaggtataaa atgaaatacc taccggtaaa aacaagttga attcaggaac
    20161 attatagaaa atgtcgaacg aatcgaatac tgcccgagta gcgtcattaa agaagttgaa
    20221 atacttaaat gagaacaata aacccagatt tgaaaacaaa ctgatcaaca tatatggtcg
    20281 ccgcttccgt ttatcggttt gcttcgccat tgcgttggct gttaaataat caactgttgt
    20341 tgagaataca atcaggaagg catactcggc cttccagctc atataaaagt agtaacttga
    20401 aaataacaga aataaccaac gccacttgta cggaatagag aagtacagga agatggctac
    20461 cggtaaaaaa atcaggaatt gtaatgagtt aaaaatcatg gcaggttccg aattttaact
    20521 cactatcaaa ttttgttact gtcagtgtct attaaagact gactttcctg ttctttatgg
    20581 tatttttcaa taacaagttg cttctgacga gcttcttcta cgctatcacg aatatacttt
    20641 ctgaaagcta atacgatgct tgtaagtata gttcccaaaa taaatccaat gatcatgaac
    20701 atcgtagttt gctctttttc tttaatggta gcaaacttcg gttgatcaat catatccaca
    20761 agcggtgtct tgttttgaag atcgaaaagc gctaattgca actgtgcgta agtagtagaa
    20821 tattcagtgg tgataagtcc tatttcacga atcaacctgt tctggttcac atatccggaa
    20881 agtttaacct gataagcact tccatcctga atccttgcca acaattcctc tttcccatca
    20941 agtaacgctc tgagtgagtc cgctttttcg gacatcaggt taacagtttt ttgctcgttg
    21001 actacttgtg cattgatata gtaattagaa cctgcataag cgaggtaatc tgccagatag
    21061 taagaaaaat ctctactaag ggaagacact tccatttgat tcataccaag gtcatcactg
    21121 aattctgttg agatatcatt ttcaataatc tcatcataaa taacagtaag acagctatcc
    21181 tcataaacgg tcaatgaatc taatgagcca tgttccagtt tgaagttttt aacctttttc
    21241 tttttagccc atcgtggata catctctaca tagaaattgg ccaaatagtc ctcacgaggt
    21301 gcaacaccct tctttactgt caaggtgcgc ttctccatca acgcctcctt aacaagggta
    21361 cgtgattgaa gaattccaag gaacaactca ttggtaaact ctgtaccggt tgtgatacca
    21421 ataccaaatg agctcgccaa agatgccagt gatgaggttg acgaaccttc agttccttta
    21481 accgcgaaat cggcatgaga aatataatat tcctttttcc aaacagcgaa gaatcgcaat
    21541 cctattgcca acaaaattgc ggctataaga atcacccacc atttttttac aaggtacctg
    21601 cgatactctc caaatttagt aaagaggtca ccaatgttta tttcatcatt atacggacga
    21661 tcgtattgca tagttgtggt tgcttatctg agcaattaca gttatgatgg tacttttaag
    21721 atcaatacat atatagttgc aaagctcaga atacctgcac taagtgtagt ggcaaaggta
    21781 ttccagtcaa atggctccct tggtgctgat ggcgcttcag gtgcatccgg cttttcttca
    21841 ggtttctgtg gaacaactac ctgacaacct tttaccttta ctcgagggta aaaatgtatg
    21901 aataagagat tatgcgttct gcgcacataa ccattcggtt caacgatata ggtttcactg
    21961 cgcttagcat catcatcaaa gtttttacca tatttcttta taaagaaacg agcagaacga
    22021 cctttttcaa atggaacaat tacagtgccc ggatcgccaa caaacggata tttaatttca
    22081 cctttcaaag ctaccagatc acctgcttta ggaacagtaa tcacatctcc ttcacgtaat
    22141 acatagttat attttgattc aggattttcc aatgctttat gaaggaatac cagtgtagaa
    22201 gcaacatcat ttctgttcat tgttgcacct tcagcatatg cccactgtgt aagtccgcct
    22261 gcgcgttcta tcacatcagc aagtgtttcg gtgcgtgatg taagtgtata tacacccggg
    22321 aatttaacct cgccaagtac tgtaacattt ttctgatatt cgaattcagg tgtattgcga
    22381 acaaatacct gatcgtatgg ttgcaattgg aaagtgtttg caccatcttc caattcaagt
    22441 tgcttgccaa tgctgataga ttgaataact acacgtgtag gttcattgtc agaattcgct
    22501 tcattgaaat tcacaatacg agaaatctca attcggctat tagccgcttc caccttcaaa
    22561 cctcctgaat aatacaacag atctttcagt gtcatttttt catagtatgg gaatatacct
    22621 ggaaccttca cggatcctga aatagaaacg ttgaatgttt cacggaattc ctgtttgctg
    22681 aagatatcaa tcacatcaaa ttttgttaat ttgaagttga atgcagaatt cggattatct
    22741 ataacttcct gcaaatcgaa tggaatatac acatcgttca atttgtcatt cttgcgaatt
    22801 aaatatgcac gatcagtata tgcatcgtaa ttcaaacctt tagctttcgc tatgagatca
    22861 gaaatacgca tatcttctgt aagctgatag tttccgggga aacgacaact accttgaata
    22921 gtaacaatat tctcaactac ctctggaatt cttgcaaaag tgattttatc tccatccaac
    22981 aatgggaaat tcactcgctc gcgctcaaga ctgtcgaggt tgaaatccaa taccacattc
    23041 tcgttattga tgtaacggaa aatagttacg ttacgcttat aagcagttga acgaagaccg
    23101 cctgcatatg caatgagttc gtttaagttc tcacctttta ctaattcata tttcaaaggg
    23161 cgattaattt ctccctgcac atcaactacg cgaccaatgc cattcacaat gatataatca
    23221 tttgcttcaa gataagtgtc atcagtggaa cccggattca tcaaaaattg ataaacatcg
    23281 agtgtcttaa taaccttacc atttcttttc acctgaatgt tacgaactga accgatatca
    23341 gtaacacctc ctgctgcaac taatgcattg aatgcagtat taacagatga aatattatat
    23401 gtgcccggtt taactacttc tccgacaata ctaactttta tcgtctttga atatgttaat
    23461 gtcatatcga atgtcgaatg cacaaggtcg aaagaagatg caaaacgctg acgaacaagc
    23521 tgacgtgctg cactgaaagt aagtcccttc aaataaatgc gacccgtttc aacggtggtg
    23581 atgtaaccat cttcacttac agtaagatta ccattatagt aagcccaacc gtaaatagaa
    23641 acatttatct gatcaccggc gccaatgata tagttatcgc taacttttgc attcggaacc
    23701 ttatcaaata gatcgataga gccattgcgg aaaatatcct gaccccaaat tcctgccgga
    23761 gcgcttgaag gagtatcatt tgatatcgcc tgctcaatca cctgctcaac taccggtgga
    23821 ggcaatgttg ccgtgttggg tgctaaagta ttcagctgat tgttagcagc ctgaactgca
    23881 ctttgctgag catcctgaat ctgtgccgct gcttcaacag cagtagaagt tgaactacct
    23941 gttgtgctca tgatggcatc aatctcggca gagtcaaatc caagtgattc taatgtcgaa
    24001 gacgatactc ccggaactat gggagtgctc tgtgcataca gggtttgggt gaagcctaca
    24061 actaatagaa gtaaggttat tgaagaaaac ttcttcatag agtttttaaa atttcgtgca
    24121 aagataaggg tattcttaca atcggtcggt atacgacgaa aaacaatttt tcctttcata
    24181 tcaagggttt cagtaatgaa caacccctcc aagacgaaca gtaaagcgtc ttagttgcgt
    24241 gatttcgcta atatgtaaat atttaactta tggagtcccc gggaccggca tttccttccg
    24301 cggcaacgaa atggagcgca ccatccttac cttcagccat cagtatcatg ccattgctct
    24361 ccactcccct catcttgcgg gggagcagat tggccagtac caccactttt ttgcctgtaa
    24421 gcgcttccgg actgaaatgt aaagctatgc ccgaaacgat ggttctcacc tcgaaaccta
    24481 agtctaccgt aagtttcagc agcttgtcag ctcccgcaat acgctcggca cttaaaacca
    24541 cacctgaacg caaatcaagc ttagaaaagt cgtcatagct aatggcttcc ttgatcggat
    24601 tggccgcaat aatagtagtt tccgctgctg tgactgcttc ccgcgacctc cgtatagact
    24661 ccagggcgtc tagtttagac tggatgacct gatcttcaat cttttcgaac aaatagaatg
    24721 gcgaacccaa tcgggcgccg ctggtcaaat tcaccccgcc aaccgccatt ttgggagctg
    24781 cgggtagatt gaaataagcg ttcagcctcc cggcactgaa aggaagcaaa ggctcacata
    24841 aggtggccat atcatacaaa ctctccaggc aaatgcgcaa aacgccttct gttcggattg
    24901 gatctgtctt atatagtttc catggttcaa agtcggtaag cagcttgttg ccaaggcgaa
    24961 caatatccat gaaagccatc aaagcatctc tgaacctgta agattcgatg cctgtgcagt
    25021 aatcattaaa aagttgagct acttgagcac ggtaagccac tacataagca tcttgctcta
    25081 cagaatccac ctgagccggc acttttgagc cataatactt ttctgtcagc accatcactc
    25141 tattggcata gtttccgaaa atggcaacca gctcattgtt attccttgcc tgataatcct
    25201 tccatgtgaa ctcactatct tttgtctcag gcatattggc acacagcaca tatctaagca
    25261 cgtcttcctt ccccggaaac tcctcaagaa attcatgcac ctgcacattc cagtttcggg
    25321 aggttgacat tttatcgccc tcgagattca tgaattcatt ggcgggtaca tttgaaggaa
    25381 gtatgtattc gccatgactt ttaagaatac aagggaaaat gagacaatgg aatacaatat
    25441 tatccttgcc tatgaaatgc aataatttgg tgtcggcact tttccaatac ggctcccagt
    25501 cctttcccgt attcaatgcc cattgcttgg tagcgctgat ataaccaatc ggagcatcca
    25561 gccaaacata aagcacttta ccttcagaat ttggcaatgg caccttcaca ccccaatcaa
    25621 ggtcgcgcgt aattgccctc gggtgtaagc cctctttcaa ccagcttgca cattgaccta
    25681 gtacattctt tttccattct tcagttctac cctctccttc cacaatccaa ctttgcagcc
    25741 aatcctgaaa tttgttaagt ggcagatacc aatgggtggt ttttcgctta accggcggct
    25801 tattgctaag cgtagaaacg ggttctatca agtcggttgg tgaaagtgac gttccgcatt
    25861 tctcgcactg atcgccataa gcatttggat ttgcacatac aggacaggtt cctttgatat
    25921 atctgtcggc aagaaactgt ttaaattcct cgtcataata ctgctcactt tcgcgctctt
    25981 caaacacccc tttatcgtgc agtgtcttaa aaaactcctg cgaggtctca tgatgaatag
    26041 gcgcactcgt tctatgataa atatcaaaac tgatacccag gtcgctgaat gctttgctga
    26101 tgatggcgtt gtacttatcg ataattgcct taggcgttgt attttctttc tttgcctgta
    26161 tggtgatggc agcaccatgt tcatcggaac cacagataaa aaccacgtct tttccctgtt
    26221 tacgcagata acgcacatag atgtcggcag gcagatacgc accggccaca tgtccaatat
    26281 gtaatggacc attggcatac ggaagtgcgg atgtgatcaa atatctttta ggttcttgca
    26341 agatttaggc aattttggac gcaaaattac acattctatg cgcaacagta tgatactcaa
    26401 acaaggagat aagattggat tggtagcaac cgcgcgttgg attgaacaag aaacggtcga
    26461 tatagccatg gcgcaaatca gcgaatgggg atttattccc gttgttggca aacacgcctt
    26521 atcaaaaaac ggtcagcttg ccggtacgga taaagcgcgc gcttccgacc tgcaaatgat
    26581 gctcgacgat aataccatta aagcaatatt ttgtttgcga ggaggttacg gcaccatcat
    26641 gatcatcgat cgattagatt ttacattgtt cacgcaattt ccaaaatgga tatgtggatt
    26701 tagcgatatc actattttac acgcacatat taacgacaaa ctcggactgc caacactgca
    26761 ttgttctatg ccttacagct ttccaacaaa cacctcagat gctatcgcac aaataaatgc
    26821 aattttaaag agtgaatcaa ttaccatttc ctcagaggca acatcctata ataagatagg
    26881 cgaagcttcc ggaattttag taggcggaaa tctttccatt ttatatagtt tgttaggtac
    26941 gcgctatgga ttttcaactg ctggaaaaat attatttatt gaagatcttg atgaatacaa
    27001 atatcacatc gatcgtatga tgatgagtat gaagcttgct ggaaaactgg atcatataaa
    27061 aggattaatt gttggcggaa tgagcgacat gaaagacaat cctgttccct tcggaatgga
    27121 aattgaagag atcatattgg agcatgttgc gcacctgaac attccggttt gttttaattt
    27181 tccggcaggg catatcgaac acaaccttcc tattcgatta gggacacagg cagctctcag
    27241 tgttaccact tccggtggta agctaagttt tttgtaacgc tgaaggcata caaataaaaa
    27301 gacctcccta caagaaggag gtcttatata gataagcaca acagccttgg taattaattc
    27361 actttcgaag tgaacgggaa tttgttgcca ccatacgatt taaggtcgca ggtgatgcgt
    27421 ccaacacgca ggtctgattc aatgcgcacc ggaattctgt tttgatcatc ggtaacccat
    27481 atggtcatac cttcaccttc tttaaatact ttgccttcct gcaacaatgg ttttaatttc
    27541 acacaattga atttaccgag tttagtttca tacacttctt tgccatcata gcgcacgtac
    27601 atatcatatt gtgcatcatc aattgccatt tttacaggta ctttatctcc ttttttcaat
    27661 ccgctataat caatagtacg aatccaataa aatgtgctca ataaatcgac accgcatgga
    27721 tctgcattat aaattgtttt agtttctttc ttgcctttgt ggttatcctg gtatgaatag
    27781 atgatatttt tttcacgatc ccaatcatag gaacgataaa aagtatatcc accttcatat
    27841 acatcgcgac tgaatttata agactgcaat gtttgtttat tcatgtaagt atcggcctta
    27901 tcgcgcactt taaaaaacca gtcgaacgat ttgtaactga tagcctcacc tgaagcgtgc
    27961 aataccggaa ttcctttgaa ggattcttca gtaagtttga agtacacttc accaccacta
    28021 agccatagtg ttccccaatt ataatacact ttataggtca gcgattcacc tccctggaaa
    28081 gccatatgtg gtatttcaca actttctgaa ggtgttacca cacgcccgga cgaaaagtct
    28141 tcgttggtcc ccgggacaag cgcgctaaac gagagcgcag tgatcaaaag tggtttaatc
    28201 atgattgtcg gtttttaaat atgttgaacc ttataataaa aatataacct gctattgccg
    28261 gtataatcag attcaggatc cagatgcttg ttgttgctgc cagtgatatc tgtaacgaaa
    28321 catcacattt cgacagtatt aatgctgcca ccggcaatct ggtcaacaag tctgctatcg
    28381 cgaaactggg taccactgtt tgagccagaa aaatcactgc aattgccgct aaacccgtca
    28441 tcaccgataa ttctgccccg aacacattga gtaaaatcaa atactgtgcc gcatatacac
    28501 tatacctcag aaaagcaagc aggagtagcc ttgagaggtc gtttgcggaa tagtcgccaa
    28561 ggacggttgt atagggatag atgcgcttca gaaactttgc cttgcggaaa aggttgacca
    28621 ttacatccag gttataatac gccagcagga gtaaaatgac agcgattacc gctaccaaag
    28681 cgagggcgcc agtcaccaac aggtcaattt tttctatagc ataaacatac caaagtaacc
    28741 ctgcgattcc ggcaattacc gtaatcagat tctggctgaa actacctatc aaagaaacaa
    28801 ttgccccctt aatgcggaat ttccttggca catataaaat tcttcctcca aattccccca
    28861 cgcgattagg cgtaaacaac ccgagtgtca ctccagtcca aacggcttta aatgcgcgaa
    28921 tgaatttaat aggtgtgata ttatctatta cctttttcca tttccatgct tcaaatcccc
    28981 agttcataat cataaggatc actaatagaa ttccgtatcc gatagccttg cctgcgccga
    29041 tgctcaaaaa ctgctcagga atggcatgta cattttcatt gaggtacacc tgtttatata
    29101 aggcaaagag caatatgcca aaaaccacta cttttatggc aatggaccac cagagtgttt
    29161 ttttattcgt ggttttcata ttttataatc aatggctgct gcttcagaaa aaaagatcat
    29221 tctcggtgta gaccctggaa caaacattct gggatacagt attgtgtctg cggataaaaa
    29281 tacaatcaaa attatcatca atggtgtgat acagttaaaa aagactgacg atcacttcac
    29341 gcggttgagt atcatctttg aaaagctaag tacctatatt aaggaatatc aacctgttgt
    29401 gatggctatt gaggccccgt tttttgggaa gaatgtgcaa tccatgctta aacttggtcg
    29461 tgctcaaggc gctgctattg tagctgccgc aagcaacgga ctggaggtta ttgaatatag
    29521 tccccgtcgc atcaaacaat cgataacagg gaaaggaaat gcgagtaaag aacaggttgc
    29581 caaaatgtta caaagcatgt tgcaactgga gtccatgccg aaatattttg atgaaacaga
    29641 tgcattggca gtagctgtgt gtcatgttaa tgcatcaaag cgtccggccg ttgcggggaa
    29701 gcaatacaaa ggatggtctg atttcattca ggccaatccc ggaaaaataa aatagattat
    29761 aaataacttc tgatcttctc ggcaattgag gcgagttctt cctgcgacat ttttaattta
    29821 ggtcggctga aattgatatc atccattgtg cgcaaaggaa gcaggtgcac atgtgcatga
    29881 ggaacttcca aaccgattac tgagatcccg attctttcgc aaggaatcgc tttttccatg
    29941 gcgatggcaa tattgcgggc aaaaggtaaa attcctgcaa gatcatcttc ttccatttca
    30001 aagatatagt caacttccac tttaggcacc accaaaacat gtccgggtgc caaaggcata
    30061 atatctaaaa aggcaaaata tttatcgttt tctgctattt tatgacaagg tatttcgcca
    30121 ttgatgatgc gtgtaaatat ggaaggcata tcacattgat atttcgagta tcttaaattc
    30181 cagcactccg gcaggtacgg taattttaac aacctcacca acttttttgc caagtaatcc
    30241 tttgccgata ggcgaagtga cagaaatttt tccgcttttc acatcagctt ccttttcaga
    30301 taccaaggta tattttattt cgcgtttgaa cttgatatcc tgcagcttta cagtggtgaa
    30361 tacagatact ttagaagtat ctattttaga ttcatccagt atacgtgcat ttgcgagcag
    30421 ttcctccaat tttgcaatac gcatttcaag taggccttgt gcttcttttg cagcatcgta
    30481 ttctgcattc tcagacaggt cgcctttttc gcgtgcctct tttatctggc gggccatttc
    30541 tgcgcggcct ttggtcttca gctcctggat ttccgcttta aggttatcca tcccttcttt
    30601 actcagatat gatacttctg ccatatgctt aggtattttt aaaaaaaaga aacgtttccg
    30661 gttaagaaac gtttccacaa aggtataaaa gaaatctatc agaaactcag acgcagacca
    30721 aagcaatgag atccttgata ctgttgtgcg gttctgtatg agtagctaag tgctactaag
    30781 ttttttccaa gatcatctat tggatattga actgttacac cggcagcaat gcctgtagat
    30841 gctgttgtgc gatctacatc gctggtaata ccgttttcat aacgatatcc tgcgcgaacc
    30901 atgaacattt cagtattttt cactttcaaa ccatattcca atccggcacc aaactgatct
    30961 ttatagaatg agtttgacgt aaagtttcca actgcggtga ggcggttatg tgtgccgaaa
    31021 tagaaatcat aagaaagacc gatagtaagc agtgaaggta attcaaatga ttctgaacgg
    31081 aattcctgag tcattgaata atcaccatca ggtacatttc cacggaagct gagaccgtcg
    31141 ccggaatact tcattggtgt tccgatattg cgtaaagcga tacccatttt aatttgctca
    31201 gggtgtgttt cgctaccgct gatatattgg ataccaacat ccattgatac gcctatcgct
    31261 tttacgtcag agatactttc agagataacg cgaacagtaa gtccaccact gagataatct
    31321 gcaaaacgtt ttgcgtatga aattccgaag ttagagaagc gaggtttgta aactgctcct
    31381 gttccttcag ggttatcagt agttgtaacc tcgatctcgc cgaggtccat agatgttaag
    31441 gtaatgccta gtgctccatc gcctactttg gtagcaaaac caaaagtgtt aaggctgata
    31501 tcagaaccca ccaaccattc ggtgcggtcg aattccaatt gtgaaccatc catgaaggtc
    31561 ataccggcaa cgttgatatt tggtgcttcc acaccacgaa ccgaagcaat attcagtccg
    31621 taccatccgc tgctttgagc ccatggatcg atgagcaatt ctgatgctcc ggcttcaccc
    31681 gaacgatccg ggttaccggc aaacacacca gccgggatgc ataatgtcag tgctattaat
    31741 atccttttca tttctgctat gttgtgtttt cggttttaga atgtgtctaa gtcgattgga
    31801 cgcattacgc caaaccattt cactgttgtt tctccaaggt cgcctgcatc gatgtggatg
    31861 atatacatac cactggcgat aggcacgttt ttctcgttct tcaaatccca ttcgagtgaa
    31921 gtatccaagt tcgacgactt agaattcaat tcaccacctg ctgtattatc tgaagtaaca
    31981 tcgcgttcaa atctgcgcac caatgttcca tcaagagtga aaatgctgat agtacattta
    32041 gatggcaggt tggtgatttt cactgcatta tccaacgaac ttgtttcgta tgcagaatag
    32101 gcgtagtacg gattcggaac cactctaatt aaatcaagcg ctgcactagc cgtttcagta
    32161 tcatttactg aaggagcaag attcgcagtt gagaattgat acagcgggaa accgaggttt
    32221 tcgcctgtta caaatttatt agaatatggt ttcgttacac gtaattgaat agtagtctca
    32281 gtaggaacta acattggtga acctaccgca cttgtatatc ccgtattcag gaatggcatt
    32341 gaaacccata caatattttt gaaaatatcc ttgtaaacag aagtttcagg tttagaacca
    32401 cctatccatt gcaaagaatc atggatgttt tcgcaaccat cgtattgagt tttagaaaca
    32461 tacatatagt gtttaccacc ataaatagga ttgaatgttt catcaaagaa tacatctgat
    32521 gggttgaaac acatgtcttt accgcggcca tctggttgaa cggcacatcc gtcttcacca
    32581 aagaataagt taaggcgagt tcctgtttca acatcaattg cataacccgg gaaccatgag
    32641 taaccaactt ctgaaccttc ggcaacacca tcctgattta cggattgttg ctgacgcata
    32701 tccaatttaa tctcaccagt agggcgttcg cattcaccag tttcaacaac aatacaacgc
    32761 gaccatttgc tgcgatctga tgtaaatacc acatccacac caattgtttc ttcaatcgga
    32821 atttgcggac gcagacctgt gatagaacgg aatggaacat atggtacttc ggcagtacta
    32881 aaggcaacca attctgttgg cgaccatgtt ccgcctagca cgccttcaaa cacctgattt
    32941 tcatccgggt taggcaatac atctacgaaa gtcccatcag caataccgga acgtatccag
    33001 tcgagtaacg taccatcatt atcaggaacg cctgtcaacc actgtttatt aggatcagta
    33061 taagataatg ttgcgctaat aaatccgtta ttcacttctt tttcaacaaa tgagccgcta
    33121 acagtctgac cacccggatc aaacacatta tgcacagtta ctgcaagtcc ccaatctgac
    33181 aatacttgtt cgttcacaat attgatagga aaatcggaag tcacagaaac accttccgtt
    33241 aagttggtta atacccacca ggtactatcc ggatttaacg gattactgtt tgttgaatcc
    33301 accaaacgca attcaaagtc tgctgcaggc acccgaaccg ggtcataaac cataacatca
    33361 acaggcgccc ttctaccttt ataggtaggg tctttcatga atgaattggc aagtattgtt
    33421 tgaatagatg catctgtcaa ttcctggaaa ttaccgccat tgccgacacc ttctatctta
    33481 gtgatttcag gaccatcgcc atatgctgca tttaaaacca taccgccatt ttccggatcc
    33541 ggtttatgtg gaattgcagt atatactttg atgtttttac gaccctcaag gtaaggacgc
    33601 ttttgtgaat ttggatctgt tggatcataa tgaatgaaat cattatatgc ataggataca
    33661 acagagaaat aatatgtttt gaaatttatc aatttcgttg tcccttctgc gaatgcatca
    33721 tcggtaactt cgaaggtatg ttttatacct gtgttgttac ctgcaaccat taattcagga
    33781 acatcagctc ctatcaatac atcaaaatca tagttcacaa ttttatcgat accatctttc
    33841 tgatcgcatt gataaatcaa tttcgctttt gttacatcgc catattcaga aggagaaacc
    33901 tgagcgttcg ccaactgata aattttatat ccttggaagt tataagtaga atcagtagca
    33961 cccggatttg cttcaagaat actatcagga agctgagaaa cgatacttac aatcagtgga
    34021 tctgtttctt gatatgattc accctggttg tttgacgtaa caccatttgt taaagacaat
    34081 actatcgatt gatctaactc acggatatcc atatcaggtg catccggacc accaattaac
    34141 tggaagtcgg catcaaacaa tgcctgtgct ttttggtcgg ccaaacgagc aaggtcaaat
    34201 gaagtctgac aaccacctga taccggaggg cgcacccaaa tggcaccaac tacgatctca
    34261 tttttcgcac ccgggaagag tgtgaaagga ccggcagatt gcagataacg acggtcggca
    34321 gcgggattgc tttcagtaca ctcgctccaa cctgtaggat ctgaaggatc gctagggaag
    34381 atgaagtttg aaagtgttgt tccaccgtag ccgtttccac cataggtaaa tggagaacca
    34441 tctttccagg ttcctgacat ataaccatag tagtgagcag caacctcagg gttaccgatt
    34501 atcgagaagt cattgttata atagaggaaa gccgacattc cgaggcgcac gctatctacg
    34561 atagaaccgc cttcagtgat gtatttaatt ggcccctgga agaagtctgt tccaaagatt
    34621 ggtgggtttg caccataatt tggagatgta ggaccatcaa caaggtcgcc attgtagcac
    34681 attccgagtg ataaagttgt atcacaacca acccagtcat catcataggc tcccaagtcg
    34741 gagtctaccc attgaccgaa atatgttgag tcgagtgctg tagtagcata gttgataacc
    34801 ttataacgat agaaggtcat gtcgttgatc tcatcgtttg tttggaaacc aaatgccata
    34861 gcttgaattt ctataccgat agcatcacca cctgattcag tgtggatgtt tcctttatcg
    34921 ttataaaccc agaagatagc ctggtcgcca tcgatatcag gatattcgcc taattcagga
    34981 gaatagatac catcgaagtt tacgtcaaca aatggagcaa ggtctttttg aacgtcaagc
    35041 aattcacctg ctttaccggt agcatactgg ttacctactc ccggccattc gcggatgatt
    35101 tgaggtattt caccttcggg gattactgat gcagtttgag aaacacccgg agttccgcca
    35161 taaacaagaa tggtttcaaa gaaatcatct acatctgtac ggttgagttt ccagtgacgg
    35221 tcgtaagcat tacaagttgc ttcttcaata gttccgtcgg catcaagtgg tcccggccaa
    35281 aagtcattac cactttgacg ataagtctga gcggcaattt tcaactgacc accggcatcg
    35341 ataccaccga tccagagtgc gcctgcaaag cttgagctta caggaataac acctgttgaa
    35401 gggtcaactt taggaatttc atactttgcg tcattctgaa ggtcccacca gaagtcgcct
    35461 gcccctaaca agcgggcgcg aacgttatta atatccagat cgatctgcgt tgttgcatca
    35521 gcacaaccgg cattcaaacg cgcatagttc gtgctgcgtt taccggtgat ccctgcattt
    35581 tcttttgcct gtgcagtaaa gcccaggaaa agcatgagca taacaatcca aatctgcttt
    35641 ttatgtaaca tatctaccag tttacttttt ttcggattag aaatcaaatg aaacacctac
    35701 acggatcctg cgaggaatac tgtagttatc aggattatcc attttaatat tatacaaatc
    35761 gacaaatgct gcaggattta cctgtgagct gatcgcttcg ataccttctg ctgatgtcaa
    35821 ataaccatca tcagttgggt taccggtata tggataaaca gcaatcacgt tttgtgcatc
    35881 gaaaaggttt tgcacccaca gatatacatc catagaaaca gggcgttcac ctttgcctaa
    35941 gccaaattcg aaagcacggt caactttaat atctgtgcga tagtgccatg gcaagcgtga
    36001 accattgata gctccttcaa gtgaagaacg gttgttgata ccgaattgtg ctgtaggcaa
    36061 tgggttcgac tgacgcgtat aaggttcacc tgaacgcgca ttgaatgtaa cgttcatacc
    36121 ggcattgtct aaccaatcag gctggctgcc ttttggattt tcaaagcgat agtcgagcac
    36181 gagtttaatc gcgtgacgtg aatcatatga caatggaata attgtgcgga ggtttggttg
    36241 tcctgcacca actaagttga tctgtgatgt tgcactagaa cctgttccat ctgcaaactg
    36301 caatgtatag cttgtagata atttcaagtt gcgtgtgcga cgagcaatat cataagaaat
    36361 agaaagtcct tttactgttc caaagtcgag gtttccataa gtggtgtatg acagtggata
    36421 tgcataaggc acttgaataa cctgaatcat atcgcgcaat tctttgtaga atgctgatat
    36481 tttgattaca gatgaagtag acaactgttg ttggaaacca acttggtagt cgattgtttt
    36541 ttctggtttc aaatcagggt tatccaacaa tgcgcctgca ttttctagaa agaaataata
    36601 atcggcaggt gttgttgttg ttcctgttgg aggacgctga gttaagatat catagtgagc
    36661 aaagaacaat gcctcacgat cttccacttt agaaataggg aaagagaatg cgatacgagg
    36721 catgatagta atctgcggag tataatcttc gaatgatgaa ttcggatcat aattagcatc
    36781 tttaatatcg gcatcaggat ctactacata tggtgtgata gtaccagttg aagatgtaac
    36841 agcaattacg ttagggtcag ttacttcttc gccatcggca ttaaaccagg tatcttcatc
    36901 acgataacca agaatagttg gtgaaggaga gttaaagtca tcaacgtaaa caacatagtc
    36961 ttgaccgatg ttgcccggat ggctgccatt aggattcaaa gaaccatcaa cttcagcagt
    37021 gctgtaaaca tcatatatag aatatttatc gatcaacact ttgcggtttg catcgtaacg
    37081 gtcaacacgc acacctacgc ggaatacgat atcgttgaaa ttgaagcggt cttgaatata
    37141 acctgctgta tacacaggac ggaacgcatc gattggacgt gttttaactc cattatcgtc
    37201 ttcctgcgta aagaagtcgt tgaaggaaac tgttcctgtt tgagcattac ctaaataatc
    37261 ataaccatta tagaacacca gtgaagaacc ctgattgagt aattcatcgg cgctgaataa
    37321 attaagatca aaaacatcga gtggtaaatt atcgatattg atataatcaa gatcgccaac
    37381 agcataaccc atgctattta gtttatcgcg aagatgcgca tcgaaataag cttgatcatt
    37441 agcaaggctc aaacgattgt atgtgatggt atcaaattca ccaaaataca aatcaggatt
    37501 gctgagatat tgatcgtaag tgtattgctc accatcgatc aataaaattg gattatcagt
    37561 atctaaagtg agaatgtgtt tgtttgtcaa ctgacgtgca atggtccaca aaccaattgg
    37621 gttaacgcca taaaaacgtt caacacgttg ttggaattca aaaccgaatt ctatactgtg
    37681 tttcccaact tttttagatt ttggattttt aaggtcgagt gcggcagcaa agttgaggct
    37741 atattggtcg tcatcagatt tgctgtagtt gaaccaaggc acaccatagt tccaccacat
    37801 attataagta gaaaggaaca ggtttgatgc atcaccattt aataaaccac cacctaactg
    37861 aatatcaaaa aggcttgatg gaggcaatgc gctaccattg atatattctg tagtatattc
    37921 tgcgagtgca gggtttgctt caccgggagt aaattctacc agtgtatcgg cataaccggc
    37981 aagcacccaa ccagtaagac cggtagtctc atcagtggta taaaaatata ccggtgcttt
    38041 ataagtattg aattgtccga tgtaaccata agcaaatgga ttcatgccat ggtctgcatc
    38101 ttgacgacta gattggaatt tggtgtaatc gagctggatg ctgtaatatg cattaccaac
    38161 aactgattct ttattatcaa cgctgcgttt ttcaggaaag cgctgggtaa aacgaagata
    38221 accgcggtaa gtattttgat catcaaccgg attattttct gcattaaata atgcgaagtt
    38281 tcttgaataa gtattagcat ggaatttacg ccatgacaat cctaactgaa gattagtatt
    38341 cttactgaat ttataatcca gttttgagtt caatgaaagt ccgtagttat ttacattgtc
    38401 gcgtgcacca atagtttcaa gatcatcttt agtgatgaat tcagaatttt tattgaagcc
    38461 ggcgcctgtt actgaaggac gcagagggtc gtcgataaga ctttgcatca catcaccctt
    38521 aactgcccaa acatcagtag ccgatggatc aggatctttt tccatttcga gctcacctgc
    38581 aaggaagaag ccaaatttag ctactgcaga atcagtgcct ttatttttta cgataaaagg
    38641 accggtcagg aataatgaag caaggttata tccgtaatca tcgagatact ggcttgatgc
    38701 caactcgata cttccattat aatctttggc aggacctctt gtggtgatgg tgatgaaacc
    38761 acctgttgcg tcaccatatt tagggtcaac accaccggtg ataacctcga gctgttcgat
    38821 agcatcttga ggcaatttca gtgatcctga tacacgtaca ccatcgatga tatattcggt
    38881 agcatcgccg cgtgaacctt tgatgttcaa gccgccacct tcatcactct ggtatacacc
    38941 ttcttttgtt gcagcgatgg aggtaacgtt acgggtggcg atcttttgga tatcctcctt
    39001 cgtaactacc ccgcctgtgc tggtatttcc tttatcaacc aatggtgcct gatatttcac
    39061 gactaccgtt tgaagcactt gctcgctcaa aacgatatcc ggcaaaaaga cgataccttg
    39121 agataccgta atgcctgtca acctttgttt caaaccaaca taagaagctt caacctcgta
    39181 agttcccggt tcgagcgccg gaattgaata cttaccgtca aagtcggtgg tagcaccggt
    39241 ttggagaacc ccgttcttgt aaacaactac gttggcaaag ggaatacctt cgcttttgtc
    39301 gttggttacc tttccctgga tctcaccgga ctgcgctgct gcagatccca cacacaatac
    39361 tagtacgacg agtgcgtaaa ggagtttatt catagaccga aatgtttatt taaattgtgt
    39421 taagagtcgc taaaatatgt aaataattga aaacaaaggt agtggaagga atttatatgt
    39481 gcgtcataac ctcgtcacaa taacttcata tttaggtcac atggtagccg ataaccctag
    39541 tgccccaagc ttttcggcta atattttgga caatttaaga tacgattcta ccgtccatcc
    39601 tgcaacgtgc ggagtaagga caatattttg tcggcgacac aattcctcca tctgctcctt
    39661 atccttgcct gccaatgtat agaattgctc attttcgaat acatcgagac ctgcccctcg
    39721 caccttcccg ctgtcgagtg cctccatgag atcggaggtg ttgacaattt ttcctctgga
    39781 ggtgtttatc agccatatgc ccgggcgaca ttttgccagg aaggccgcat tgcaatagtg
    39841 catggtttcc ggattcaagg gcaggtgaaa actgatgaca tcacattcca attgtatctg
    39901 ttccatgctt acttcctcaa cattttcatc gccaaaacct gcaatatact tatcatgagc
    39961 aagcacataa acccccatcc cgcgcagttt ttgggcaaag gcgctaccgg tatgaccata
    40021 gcctataatg cccaccgtac gacccatgag ctcaaaacct cgattttctt cgcgttgcca
    40081 tacaccggca cggatatcgc gatcggcacg ggaaatattg ttcaatacgc ttaaaagcag
    40141 tcccagtgca tgttctgcaa cagcattggc attgccttca ggactgttaa ctacatgaat
    40201 acccttactc tttgcatagg ccacatcaat attttccatt ccacttccgg ctctggctat
    40261 gaattcgagt tgagtggcct tgtcgataag ggtgggtgtg accttgattt tggtgctcaa
    40321 tgctaatcca tggtaattgt caatgacctc cgcaagcccc tcatttgtaa tgtcaggctg
    40381 cacatcgacc tgatagcctt gtgaatgcaa ccagtcggcc agataactat gcaggtagtc
    40441 agtaataatc acgcgtttct ttgccatatt attttgctgc ttgaatcgct tttgtaaggg
    40501 tcaaaaattc agagacgctt aattcctcag cacgcttgcc tgcaaaatta agttgatgta
    40561 aaacaggaac agcattggga atacctgata gtgcattcga caattttttt ctgcgctgat
    40621 taaaagctgt tttcacaaca ttttttaatg cctttgggtc aacatcagga gctacttgtt
    40681 tccgccttaa tttgatcaca gcagaatgca ctttgggtgg tggatcaaat gcatcgggag
    40741 gtaattcaaa aaggtattcg gtatcgtacc atgtttgtat caatacactg ataatgccat
    40801 aatctttatt gccatgatct gctgtaacac gctgtgccat ttcgcgctga aacatgccca
    40861 ccacaacagg tacaagctcc atcacatcaa gtacgacaaa taatatttgt gaagaaatat
    40921 tatatggaaa attgcctatt accgctaaag gctgttgaaa atatttcctg atatcgagtc
    40981 gcagaaaatc gccttccaaa atatgatctg atggcatcag taatttttct ttaagaaaag
    41041 cgataattcg atgatcgatc tcactgcaat aaatgggatg ttccgtttgc aataaaaact
    41101 ttgtaagaat acctaagccg ggaccaattt caagaatggg aatttgttca ggcagtggcg
    41161 acaaagcttc aacaatacga cctgcggttt caggttgttt tagaaaatgt tgaccaaatg
    41221 atttgttgtg ttgaaatccc attattgcaa atgcaatatt agaggtaatg tttcaaatgt
    41281 aatggccggc aacgtgaaag agtgtgcact ataccgtttt gaaatgctta cttttgtgaa
    41341 aggattatgg aaaataggat cagaatcggc tttacgttgg gtgatgtgaa cggtataggc
    41401 cctgaagtgg tgattaaggc attggaagat aagcgaatgc ttggtttatg tacacctgtg
    41461 atctatggat cgaacaggat catttctttt tataaaaagc tcttcaatct cgaaggcttg
    41521 agttatacaa caattaacag tgccgatcag gctaatgcta aggcggtgaa catcattaat
    41581 tgtattacac aagaggtgat gattatgccc ggacaggaaa atgaaaccgg agcgcgtgta
    41641 gcactaacat cattggaggc agctaccaat gatctgttga aaggacagct tgatgccatt
    41701 gtaacggccc ccatcaacaa acacaatcta caatccatag gcttcaatta ccccggccat
    41761 accgaatatt ttaatgcaaa agcacctgaa agcgaaagtg ttatgttact catgcaaggc
    41821 gatctgcgcg ttggattggc aacaggacat attccggtga aggatgtagc ttcagcctta
    41881 actaaggaag gcttgctcag aaaactggag atcattcatc acagcctgaa gcaggatttt
    41941 gcgtcaacag gtccgcgcat cgcggtttta gggttgaatc cacatgccgg cgacagtgga
    42001 actttgggtg ctgaagagag caatattatt attccggcta tcaaggcagc tcaggaaaag
    42061 aaaatgtttg taaccgggcc atttagtgcc gatggtttct ttggaagcgg gcaataccag
    42121 cactttgatg cgattttagc catgtatcac gaccagggac ttgtagggtt caagagtctg
    42181 agttttggcg gcggggtgaa ctttactgcg ggacttccat ttgtgagaac atcaccagat
    42241 cacggaacag cctttgatat cgcaggcaag aatgaggctt ctgctgagag catgcttcag
    42301 gctatattta cagccgtaga tattgttcgc agtcgcaaag tatatcatga gatcactgcc
    42361 aaccctttga aacgcacgga agttgccagt gaaagaggcg cttaaggcag tttggggtga
    42421 aaaaaccttc atttgagtaa attttaaagg gtatttatct tcaaatacct gttaggaagg
    42481 cttccaatct tcggaaaact tccctacctt tgttcgcttt ttaagaatcc ggtggcgaac
    42541 gatctgaggg aatatgaaat cccgtttgtg gggttgaagc aaggggtaca ccacttcaac
    42601 tatgagttgg acgatgtatt cttcgggcat tttcccgaga gtcaggtgca gcaggggaag
    42661 gtatttgtcg acctcgcact ggataaaaaa gagcgacttt ttgttctgaa ttttgatgtt
    42721 tcaggtagta tcagtaccga ttgcgatcgc tgcggacagc catttgacct tcctattcat
    42781 ggtaattaca ccatttatgt gaagttaggt gataagcggg aggaggatgt agatagtgag
    42841 gaggtggttt ggattcccga cagtgagtcg atactggata tttcagagat ggtttatgag
    42901 tttgtgcact tatcggtgcc tatgctcaaa gcgcatcccg acaaggccgg aggagttccg
    42961 ggttgtgatc ctgaaatttt aaagttatta gggcaggaag aagaaggatc gcaggatgaa
    43021 aaaggtccgg atccggatcc aagatgggat atattgaata atttgaatat caattaaaac
    43081 agaatacaat gccaaatcca aaacgacgcc attccaaaac caggacaagg tcgcgcagga
    43141 cgcaccacgg agttgacatg cctaacgttt cagcatgtgc taattgtggc gctccggtat
    43201 tacctcaccg tgtatgcccg gaatgcggct cttacagagg caagatcgcc attgagaaaa
    43261 aagcctgaaa tattggggac taacccctta ttaagctaaa ccaggtatgc agaaaaccct
    43321 tgcagcaatc acaggtgtgt atggctatgt tcctgaatat cggctaacca atgccgaatt
    43381 ggagcgcatg gtagatacta ccgacgagtg gatcttaacc agaacaggca tcagcgaacg
    43441 ccgtatcctc aaaggagagg gcctggctac cagcgatatg gtagttgagg ctgtgaaagg
    43501 tctgttagct aaaacgggca acactgccgg tgatatcgag atgcttattg tggcaactac
    43561 aacgcccgat atgcaattcc ccgctacggc caatattgcg tgtgataaac tgggatgtaa
    43621 tcatattctc agctacgata tcagcgcggc atgcagcggc tttctttttg ccctgtccac
    43681 agctgcccaa ttcattgaat caggaaagta taagaaagta gttgttgttg gtgccgacaa
    43741 gatgtcgagc atcatcaact ataccgatcg tgccacttgc gtgatttttg gcgatggcgc
    43801 cggtgctgtg atgctggaac ctagcactga cggtaacggc attgctgatg cggtgctgaa
    43861 atcggatggc tctggtcgtc atttcctcca ccaaaaagcc ggtggttctc tcaagcctgc
    43921 cagtcacgag acggtagata atatggagca taccgtatat caggagggaa aaacggtatt
    43981 caaattcgcc gtgaaaaaca tggctgatgc tgcggaggag atcatgttac gcaataatct
    44041 gaagagcgat gatatttcat ggttggtacc acatcaggcc aatttgcgta ttatcgacgc
    44101 aaccgcagag cgcatgggcc tttcgaaaga aaaggtcatg attaatattc aaaaatatgg
    44161 caacactact gccggaacca tcccgctttg tttatgggaa tgggagccgc agttgaaaaa
    44221 gggcgataat attgtgttag ctgcctttgg tggtggattc acttggggtg caacccttgt
    44281 gaaatgggct tataacggct gaaaagcaca ttaaaagccg gctaagcaat actaagcgaa
    44341 tgggtataat aacctgcgaa gaattcacgc gggacatttt gaaacaagca atcactcaat
    44401 cattttaata tggcttcaac agcagatatc agaaaaggtc tttgtatcga tttcaaccac
    44461 gacatctggc agattatcga tttccaacac gtaaaacccg gcaagggcaa cgcttttgtg
    44521 cgcaccaagc tcaggagcct taccactgca agggtattgg aaaacacctt cccatcaggt
    44581 gcaaccatca atccggtaag ggttgagcgt cgcacttatc agtatttgta taacgatgaa
    44641 acaggttacc acttcatgaa caacgagacc ttcgaccagg tgaccttaga cggcgaaatg
    44701 gttgatggaa aagagttttt caaggaaggc caggagatcg acatcctgtt tcatgccgac
    44761 acagagaaac ctttattctg cgaattgcct gcccatatta ttttagctgt tacatttgca
    44821 gaaccggggg tgaaagggaa taccgccacc aacgcgatga aatacgccac tgttgaaaca
    44881 ggcgcacgca ttcaggtgcc gttgtttgta gaagaaggtg acgttattaa aatcgacacc
    44941 cgcacatttg aatatgttga acgcgtaaaa taacacgtat ggactttaaa gaaattcaag
    45001 agctcatcag aatggtgaac aagcacaatg ttgctgagct cactatcgaa aaccaagatt
    45061 ttcgcatcaa gatcaaaaca gcgcttgctg caaccacaat ggttcaggcg cctatgatga
    45121 tgccacagat gcaacagcct gtgatgatgc ctcaaatgag tgcagcacct gaaaaaccac
    45181 aaatggctgc agctgaaaca aagagcgatg ctgctgaatc aacaggtggt aataccatca
    45241 cgattaaatc tccaatggtg ggcactttct atcgttcacc cggccctgat aaagaagcat
    45301 ttattaaagt aggagatgaa atctctgtag gttcaaagat ctgcatcatt gaagcaatga
    45361 aattgttcaa tgagatcgaa agtgaagtaa gtggtcgctt ggtgaaaata ctcgccgacg
    45421 atgcttcacc ggtagaatat gatcagcctt tattcctcat cgagcctaaa taataaacgg
    45481 ttttcgcatg accaaaaaga gcagtaaacc gcaggaattg atcaggccca ttaaaaagat
    45541 cctgattgcc aaccgcggag agatcgccct gcgtattatt cgtacggcga aggagatggg
    45601 tattaaaacc gtagctgttt attctacagc cgataaagac agtttacctg ttcgtttcgc
    45661 tgatgaagca gtttgtatcg gtccgccatc ttcaaaagaa tcatacctga acattcctaa
    45721 aattatggct gctgcagaaa ttaccaatgc agatgctata catccgggat atggattttt
    45781 aagtgagaat gagatgttct cggcaatttg tgccaagtat ggtattaaat tcatcggacc
    45841 aatgcctgag cagatccacc aaatgggtga taagattact gcaaaggaaa cgatgaaaaa
    45901 agcaggtgtt ccggtaattc cgggtagcga aggattgctg aaagatatag ccgatgctaa
    45961 gaagcaagct aaacgcatcg gttatccggt aatcatgaaa gctactgccg gcggtggtgg
    46021 aaaaggcatg cgcatagtgt ggaatgaaag tgaagtggaa aatgcattta atactgccag
    46081 tatggaggcg aaagcatcat ttagtaatga tggcctttat atggaaaaat atatcgaaga
    46141 accacgtcac attgaaattc aagtggcagg tgaccgatat ggcaaagctt gtcacctcag
    46201 cgaacgcgat tgttctattc aacgtcgtca ccaaaagctg gttgaagaaa gcccttcacc
    46261 gtttatggat gataaattgc gtcagaaaat gggtgacgct gccgttttag ctgcagaaag
    46321 tatagcatat gaaggtgtag gaaccataga atttctaact gacaaacatc gcaactttta
    46381 tttcatggaa atgaataccc gtattcaggt agaacaccct gtaacggaag aagttgttga
    46441 tttcgacctt atcaaagaac aaataaaaat tgccatgggc gagaaaatat caggaaaaaa
    46501 ttatttccct gaacttcacg ctatggaatg tcgcatcaat gcagaagatc cttcaaggaa
    46561 ctttgcacca agtccgggac gcattaccag tttccactca ccgaaaggac atggtgttcg
    46621 cgtagataca catgtgtatg ccggatatac cattccgcag tattacgatt ctatgattgc
    46681 gaaactcatt tgtaaagcac gtacgcgtca ggaagtaatt ttaaaaatgc agcgagcact
    46741 tgatgaattt attgttgaag gtattaaaac aactattcct ttccacaaaa aactcatgcg
    46801 cgatgaacgc tttattgccg gtaagttcga tacttcattt atgaatagtt tcgatctgag
    46861 taatctggga gattaa
//
LOCUS       DAOQHY010000005        40609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_374_length_40609_cov_5.742048, whole genome shotgun sequence.
ACCESSION   DAOQHY010000005 DAOQHY010000000
VERSION     DAOQHY010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 40609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40609
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_374_length_40609_cov_5.742048"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..2407)
                     /locus_tag="PLL28_00930"
     CDS             complement(80..2407)
                     /locus_tag="PLL28_00930"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF67902.1"
                     /translation="MLRKSILSLTLICLGFLVRGQQYTVSGYVTDASSGEHLISATII
                     DKRDYAGTIANNYGFYSLTISGGDAQIVCSYIGYNTITKNIHLVSDTVIHFQLKPDLE
                     LETVIVTADNEDKLQDRTQMSTMEVPIEQIKALPAFLGEVDVLKTLQLLPGVSGGSEG
                     TSGLFVRGGSPDQNLILLDGVPVYNASHLFGFFSVFNSDAINKVTLVKGGFPARYGGR
                     LSSVVDIRMKEGNMQTFHGEGSIGIIASRLTLEGPIIKDKTSFIVSARRTYIDLITRP
                     IFALQDDYSGQQGYYFYDLNAKINHKFSDRDRLYVSGYFGRDKAYFHNDYQYDAETNR
                     SEESGISWGNATAVARWNHMYSKKTFGNLTATFTDYTFDIFSNFTDERENDPDTYFGF
                     EYFSGINDWSLKYDVDYIPNPMHYLKYGGGYTYHTFRPGATQFETNEPGTEDFNLELG
                     APNIFAHEFDIYGEDDYEISDVFKVNYGLHASAFLVNEKYYGSIQPRISGRYLLNPDL
                     SLKASFSTMTQYIHLLSNSGIGLPTDLWVPATDSVPPQKAYQPAIGIAYTFKQQYEIS
                     VEGYYKYMKDIIEYKDGTNYLVDGLDGWEDKVESGEGWAYGAELFIQKRSGKVTGWIG
                     YTLSWSNRQFPTINLGEKFPYKYDRRNDFEIACIYKFNDHIDFSGTWVYSTGQPISLP
                     IAKYEGMFNETIYAYEGRNGYRMNAYHRLDLNVSFHKEKEKYERTWNIGAYNAYSRKN
                     PFFIYLEYDYLTNTNNYKQVSLFPIIPNVSWEFKF"
     gene            complement(2509..3684)
                     /locus_tag="PLL28_00935"
     CDS             complement(2509..3684)
                     /locus_tag="PLL28_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67903.1"
                     /translation="MKRMIYALCLFTLLASGCKVAEKEFRKGNYDDAIDICVKRLIDN
                     PDKAEYIQILEESFRRANDGDLAAIKALHTDGNPDRWSTVYDIYQGIYVRQEKIKPLM
                     PLYLGTEERDAEFKFVDAVGELNNAKKNAAAYWYALGDEKMKSGDMHQARDAYYEYEK
                     IYRYYSTYKDLDAKIAAAREKGMAQALFVLTNQSSGNYNYDVQQAINRFKVYEDNGNW
                     FRLYNAYDPKEADFTVELVVRTIEAYPDKVTTNRYEETKEIQDGFEFLYDAQGHIVKD
                     SLGNPIKVPDYKTVSVFVTETWQEKIATMSGEILYKDANGRVMRSIPVRSDAVFQNYY
                     ATATGYYEALSAQSKQKIGGQPIPFPSENTLLLDAVKQMECNVNDVLEDYNDEYLNR"
     gene            3978..5231
                     /locus_tag="PLL28_00940"
     CDS             3978..5231
                     /locus_tag="PLL28_00940"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNF67904.1"
                     /translation="MKKQTTIRRLLTGFLFFAFLTTANQAFAGEAEGQTLFQTNCQRC
                     HLIDKKMTGPALMDVQSRWTDSTHLYAWIRNSQAFLATGDKYANDLYKQYNSIMPAFP
                     QFKDQDIADILYYIKVEGERKKAEAANTTTGGPVTTTAKNDNSTLYWILIIALVFIAF
                     ILARAVRYLDGLVRQKYDEGEADRTPFWRQRKYRPLVALLVLAFLCLLSVAMWNSATS
                     LGRQQGYAPEQPIAYSHKVHAGINKIDCKYCHVGAERGKSAVIPSLSVCMNCHYNIQG
                     VTGKDPNYPKEVYNKEIAKIYSYIGFNPETNTYDKEAKPIEWVRIHNLPDHVYFNHAQ
                     HVTVAGIECQTCHGQIQEMDVVQQQENLSMGWCVNCHRNSNVNFTTNDFYKPYEVLHE
                     KLKNGEIKDVKAVDIGATECQKCHY"
     gene            5267..8404
                     /locus_tag="PLL28_00945"
     CDS             5267..8404
                     /locus_tag="PLL28_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HNF67905.1"
                     /translation="MAEKKYWKGLEELKQEPEFMQLTQHEFHQEIPVEFFDEQNDTLQ
                     SSRRDFLKIMGFSLTAATVAAGCEMPVRKVAPYIFKPEDVIPGIATWYASTYYHGGDY
                     CSILVKTREGRPIKVEGNRDSLITKGGTSARVQGSVLSLYDTARYRNPMVKGSRGMGN
                     ATWDEVDKAIMGKLEANPNAQIRIVTSTVISPSTKKVFAKFAEKYPNTKVYAYDAISY
                     DGMLMANMATKGKAEIPHYNFSKAKAIVGIDADFLGTWISPVEYTKGYVANRKVSKGN
                     TDMSRHYQFESRLSLTGSNADYRVPVLPSELGIVALNLYDAITGGNASNGVKIQDAEK
                     QKRIAEAAAYLKANRGESLVVSGCNDKNVQVVINAINDALGNYGTTISFDGASQLRQG
                     TDASIKTLISELNGNQVTAVMFYDCNPVYDHAMGAELKEAIGKAAISVSFATAINETA
                     EACGIISPDHHYLESWNDFEPRKGFYSLSQPTIHNLFETRQMQDSLLKWSGNNIGYYD
                     FMMQAWAEGPLAGSISTTTAFNKALQIGLYQPESHRGMEGAGDIVKAMLAADAVMANE
                     APVADLSSSASAIIAMSKKASGLQVQFYETVAIGDGRYADNPWLHEMPDPISKITWDN
                     YISVSPKYASEKGLMHGDMVTFSVNGKSVKLPVAIQPGQAYGSVSIALGYGRTAIGKG
                     GAGVGVNVYPMLDASGDCVSYMASNATMEPVGENVKLAQTQTHFSINDGLSVRRIIKE
                     TTLAEYKKSAKAGNEDREEALAWNNVTFYPDYFGKKNGFNWGMNIDLNSCIGCGACVV
                     ACNAENNIPVVGKKEVIRAHEMHWMRIDRYYGGDPENPEVIYQPMLCQHCENAPCENV
                     CPVGATNHSSEGFNQMAYNRCIGTRYCANNCPYKVRRFNWFDYGSADTFGTMNDPSGT
                     ENLGLFEDLTRLVLNPDVTVRSRGVIEKCSFCVQRIQDAKLTAKKEGRELADGEIRTA
                     CQSGCPADAIVFGNNYDEKSAITTETMDERTFGILEEFHWLPSVTYKTKVRNKDKADS
                     HGTEALDIMDLFNYK"
     gene            8424..9824
                     /gene="nrfD"
                     /locus_tag="PLL28_00950"
     CDS             8424..9824
                     /gene="nrfD"
                     /locus_tag="PLL28_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNF67906.1"
                     /translation="MFVSPLREPLIQGHKTYKQISDDICAPVEAKPSKAWFIATTLSG
                     LLAINGFVMIGLTITYGIGMWGLNKTVGWAWDITNFVWWIGIGHAGTLISAILLLFRQ
                     KWRTGVNRSAEAMTIFAVICAGLFPIIHMGRVWLAFFIFPYPNTRGPVWVNFNSPLLW
                     DVFAISTYFTVSLVFWYTGLIPDLALIRDRAKSAFTKKMYNIACFGWKGTAKNWQRFE
                     SISLVLAGLSAPLVLSVHTIVSFDFATSVIPGWHTTIFPPYFVAGAIFSGFAMVLTLM
                     LVVRKVFNLQEYITLAHIEAMNKIIILTGSIVGVAYITELFTAWYSGNPYERYAFLNR
                     AMGPYWWSYWGMMTCNVVSPQLFWFRKLRRSVVFTFFMSIFINVGMWFERFVIIVTST
                     HRDFIPSNWTMYYPTYVEMFIYIGTIGFFMTLFLIFSKVAPVIAVAEVKSILKTAGDQ
                     YTGPDAIHKHEEDNHH"
     gene            9856..10383
                     /locus_tag="PLL28_00955"
     CDS             9856..10383
                     /locus_tag="PLL28_00955"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HNF67907.1"
                     /translation="MATKKIMYGLYDDEVVLIKAVKKVRAEGLKIHDCYTPFPVHGLD
                     DAMGLRMTKLHSAGFIFGATGTLTALSFILWITTNNYPINYGGKPYLSLPSWIPITFE
                     LTVLFASVGMFLTYLYLNRLAPGMKAEIVDERTTCHMFAMTFEIDDHTTDAKKTEIRK
                     VLSDSGAVEIHEKEL"
     gene            10402..11070
                     /locus_tag="PLL28_00960"
     CDS             10402..11070
                     /locus_tag="PLL28_00960"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNF67908.1"
                     /translation="MVKRNIMIALVAAVMIPAVLTSCKKASGDYPGDSYTWDMSYSRA
                     YEPYTLNPELRDSMTAMTPVAGTVPYVGNAVAGVKADSLKMALNLPYTIPNTPEGYLR
                     AATENHNPFSINDEKVVAQGQHFFNIYCAVCHGVEGNGKGFIVTEGKYTAMPPSYFDP
                     MYMAMTEGTMFHSVTYGRNAMQSYAYALSKEERWKVITYVKKMQADYAAQNAATAATT
                     TGTN"
     gene            11113..12501
                     /locus_tag="PLL28_00965"
     CDS             11113..12501
                     /locus_tag="PLL28_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67909.1"
                     /translation="MNGSNKFEYTAKLKNRFLGMLVIGVVLVVVGMLTGASMDRFWSN
                     YLQNTLFFLGISVLAVFFLAAHQIGMAGWHITVKRIPEAMSQFSKVGAVLMLVVVFGV
                     IFDYHHLYHHWTNTFIQEKEVSVEALKDYEAELAEEGHSTEHAVDYNRTWQDFQYHQV
                     APAPEHVDAAKPEPEVLENGMVVNPYYDRLIDHKSAYLNKTFWSIRSISYLFLWVLMA
                     MIIRRYSLREDLDNSDTFYQKTRKAAAVFLIIWAVSSSTMTWDWVMALDPHWYSTLFG
                     WYNFISLFVASICTTILILIYLKRNGYLTQLNENHLHDLGKYAFGFSVFWTYLWFSQF
                     MLYWYGNIPEETRWCLDRARTDYKWLFYANLLLNFLFPFLTLMRRDAKRNMNVLMIVA
                     IGLIITHWLDFYMMITPATLGTEVHFGILEIGMFIAYAGLFLFVVFGELAKASLIPQN
                     HPMYKESLDHHI"
     gene            12517..13935
                     /locus_tag="PLL28_00970"
     CDS             12517..13935
                     /locus_tag="PLL28_00970"
                     /inference="COORDINATES: protein motif:HMM:NF014809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II transmembrane
                     domain-containing protein"
                     /protein_id="HNF67910.1"
                     /translation="MATGWLIFIAILMIVVVLFQVTRTLDLVGQLRGGDTTTVSMTKF
                     HASLGMIFMVLGMIGFFWSFGHFHDYLVDHHSSDNGLIIHRMFVITLWVTGIVFVITN
                     VLLFIFVYQYRHRPGHQATHFAHSNKLEFIWTVVPTIVLTGLVVFGLQAWTDIMGDAE
                     DDAIVFEVTGQQFFWSARYPGPDGKLGPRDNDLICADNPLGIVTREWIDHRRVTLIGS
                     TKLGVKGEIEKLQDREKEINAIIASYEQRIATSPNKYKVADAKDSLEIVKKELGKIPG
                     KIEIREKALTRINKKYTEEYINQHAAEFTWGYDDPMPSEVHVPVDRQAFVKITALDVL
                     HNFYIPHMDVKMDAVPGMPTSFKFKPLITTADKRIEMSENPLWQVTKEGEKEPRWKNF
                     NYEIACAELCGSGHSAMKYTLVVETQEDYDKWISAQQSKWSQVAASLKLENGFSKFAP
                     KLEAPAAETPAAPADTTMITKK"
     gene            13957..15831
                     /locus_tag="PLL28_00975"
     CDS             13957..15831
                     /locus_tag="PLL28_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNF67911.1"
                     /translation="MSEHHAVAHHTEAHAHGHDHHGHDGHHELGFIRKYIFSEDHKTI
                     AKQFLVTGIFWAIIGALLSVLFRIQLGYPNADLTWMKPFLGKWITEEGQIAPEFYNAL
                     ITMHGTILVFFVLTAGLSGTFSNFLIPLMVGARDMASGFINMLSYWFFFISGAVMFVS
                     FFLATGPASGGWTAYPPLNALHDASSGGVLGVDYWLISMALFIVSALLGGLNYITTVI
                     NLRTKGMKMFRLPLPIWAFFLTAVIGVLSFPVLLSAAILLLFDRNLGTSFYLDAINLA
                     GEPLANSGGSPILYQHLFWFLGHPEVYIIILPAMGIVSEVLSVHSRKPIFGYTAMIYS
                     LLAITVLSFIVWAHHMFMTGMNPFIGSIFVIFTLIIAVPSAIKVFNWLTTLWKGDIRL
                     NPQMLFAIGFVSLFISGGLTGIFLGNSSIDIRLHDTYFVVAHFHLVMGVAAFFGMFAG
                     VYHWFPKMFGRYLNPTLGYIHFWGTISAAYLIFYPMHFEISLPRRYYTHSGFETFSDF
                     AHLNAFISTVAIISFALQLLFVFNFFYSIWYGRKLRESNPYGANTLEWTTPLRPGHGN
                     WEGDIPEVHRWPYDYSKHGRDFIPQTEPLSQYEIEHGEHGDEGDRDIIKKEEDENHKG
                     "
     gene            15839..16732
                     /gene="cyoE"
                     /locus_tag="PLL28_00980"
     CDS             15839..16732
                     /gene="cyoE"
                     /locus_tag="PLL28_00980"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNF67912.1"
                     /translation="MRKDSSIAGSGIGKIFAARLKDYHMLVKFRLSFIVVFSSGIGYL
                     FCGGEQSQFFLFLLGGFLITAASNAINQIIESDTDQLMTRTADRPLAAGRMAHTEAII
                     AAGVMAVSGIALLWWQFNSLSALLGALSLISYAFIYTPMKKVSAIAVFIGAFPGAMPP
                     LIGGAAANGEISNIAILLFCVQFVWQFPHFWSIAWVACEDYAKAGFHLLPTASGRSKA
                     SAVHILLYAALLIPVSLLTYFLGYTGTVSAVIVAVTGLLFFLQAVHLFRTADTKAARS
                     LMFGSFLYLPIVLIALLVDQI"
     gene            16739..17323
                     /locus_tag="PLL28_00985"
     CDS             16739..17323
                     /locus_tag="PLL28_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HNF67913.1"
                     /translation="MAVSIHKRFIVHPYKFNMWIAIVSMVMIFAAFTSAYVVKKGDTK
                     TWADIQLPQIFTLSTIVIVISSLLMHAAYVSFKKGALEWYRGFMTLTFLAGAAFLSMQ
                     IQGWYTLVAQGVYLTDNVAGSFIYVISGAHFLHVAGGVIALLVFSIRSFTRYQASNIE
                     AMKDTTINVEVMATYWHFVDILWVYLFFFFMLNR"
     gene            17336..18010
                     /locus_tag="PLL28_00990"
     CDS             17336..18010
                     /locus_tag="PLL28_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNF67914.1"
                     /translation="MEAVATTNAKDLWSGGDSPFKVSYGKIMMWYFLISDTFTFAGLL
                     ISYGAIRTSQPKHDAANPLSWPDPNEVFNAGPGFHNAPLIFVTFMTFTLILSSVTMVR
                     AVQEGHQMNRSGVLKWMAFTILGGLVFVGSQAWEWSHLIEEGMTPHGNPFGASSFGAL
                     FFLITGFHGFHVSVGVLLNSWAFISTYRGVFDRRGHYEMIEKLGLYWHFVDLVWVYVF
                     LAFYLF"
     gene            18030..18365
                     /locus_tag="PLL28_00995"
     CDS             18030..18365
                     /locus_tag="PLL28_00995"
                     /inference="COORDINATES: protein motif:HMM:NF015582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HNF67915.1"
                     /translation="MSTLTQEESRKRVWRVCGILAVVTAVEIAAALIHYNYMHDSPKS
                     FLNILFVVLSAAKAYYIMSEFMHLKYELKPMVISILAPFLFLVWGIIAFLWEGHYWLS
                     EKELWHILK"
     gene            18367..19023
                     /locus_tag="PLL28_01000"
     CDS             18367..19023
                     /locus_tag="PLL28_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNF67916.1"
                     /translation="MSSTRGRIWTIVMLVIILVLPSIAYLMLREGLHVRESAPASDHL
                     IKENLQSIPTYSGVSFRGDTISKSRMKGKVCVLNFTTHACEKSMDDNMRKLFEIQEDY
                     YGKTISFRIITNTLDPDTDGLDNMRLMSERYAGREIWHFITDPDSSGVKLYDWCKKNA
                     TDSTLNETDTQCPQLVYLVDGNGKLRGVYNILEEQQFHDLYNDILYLVNQMELHEQKK
                     "
     gene            19007..19660
                     /locus_tag="PLL28_01005"
     CDS             19007..19660
                     /locus_tag="PLL28_01005"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNF67917.1"
                     /translation="MSKRNNLIIFGVSGVAFILFVIFVYLPYAKKQDNLNKVKESYQH
                     SSYLKDPVGQLPVFSCIDQNGKVFTNDSLKGDVVVANFFYTTCEGYCPTTTKHLATVQ
                     SSLNKNIPFHIISFSLNPANDTVAALRKYADMYKADDNVWRFLHGDQEEIYQLGEQGL
                     MTIVKDTAGNFESHSDRFVLMDKMGKIRGFYRGTDSLELQALIQDINYLVFKGETNE"
     gene            19653..20213
                     /locus_tag="PLL28_01010"
     CDS             19653..20213
                     /locus_tag="PLL28_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HNF67918.1"
                     /translation="MNERTINRWIVAISILIPVAVTILFYMPAVKLNIDTSILPAFHA
                     MLNSGVAILLLLGLYFIRTGKRGAHRTSMLGAFSLSALFLISYVLYHLTSDSTLFGDL
                     DHNGIVSDAEKAEAGAMRGVYLVILLSHIFLAVLIMPFILVTLSRALTARFDKHRKIA
                     RITWPLWLYVAITGVVVYFMIAPYYP"
     gene            20238..20492
                     /locus_tag="PLL28_01015"
     CDS             20238..20492
                     /locus_tag="PLL28_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67919.1"
                     /translation="MKYRLLIMLVLVVLLSALHTDLQAQCAMCKATAEEGLREGNTQS
                     ANLNAGIMYLLAMPYLAFGVIGFLWYRNYKRKKAQEAAGL"
     gene            complement(20493..21194)
                     /locus_tag="PLL28_01020"
     CDS             complement(20493..21194)
                     /locus_tag="PLL28_01020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNF67920.1"
                     /translation="MNCIITGGTKGIGKACALLFAKNGFNVAICARDKAALNEMSAQL
                     HDANPTGKHLAISCDVRDRAQLHSFGENCLKAYGSIDVLINNAGVFLPGNITEEAEGT
                     LEFLIETNVYSAYHLTRQIVPVMRTAKKGYIFNICSVASINSYDGGGSYAISKFALLG
                     FTKNLRHELKTDNIRVTAVLPGAVLTPSWDGTPLPESRFIPADDIATLILQAYQLSDR
                     SVVEEILIRPQLGDL"
     gene            21239..23686
                     /gene="priA"
                     /locus_tag="PLL28_01025"
     CDS             21239..23686
                     /gene="priA"
                     /locus_tag="PLL28_01025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNF67921.1"
                     /translation="MRSVFVDVILPFALEKNYTYVVPDEFVGVLSPGMRVEVPFRNKT
                     YTGIVAKVHDISPQGYSPKPVLSLPDHLQVVNKTQLDLWTWISKYYMCSVGDVMNAAL
                     PAPYKLSSETVVVYNPQHGIDLLTLDEKEYLIAEALTLQQELRLIDVQQILRQKTVQP
                     IIKGLIAKGIAFIKEELKESYTVKTEKYIILTPQYRSEDALQQLFESLQKSEKQTNVL
                     MIYYQLSAKEGKVKKSVLLRRSGVTAAVLKTMIKNDIFEEVEEQVSRLQAYGNADMHD
                     AHLNVDQQKALSEIEEQFEEKQTVLLHGVTGSGKTEVYIELMLKTIAAGKQVLYLLPE
                     IALTAQIVTRLKKRFGNLIGVYHSKFNQNERIEIWQHVLNNEFKVILGARSALFLPFN
                     EIGLIIVDEEHDYSYKQHEPSPRYHARDTAIYLGHLHQAKVLLGTATPSVETYYQAKS
                     GKFGLVEMFRRHGDMPMPTIQIVDMKAEEKQKTVQGHFSSVLVGNIKHALQLKEQVIL
                     FQNRRGYAPFMICQSCGWTPKCTQCDVNLVYHKYANELRCHYCNHVENTYSVCPACNS
                     SQLIIKGFGTERIDDDLQYTFPDARTARLDLDAIKTKHGHEKLVRSFEEGEIDILTGT
                     QMVTKGLDFDHVNLVGILSADQIINFSDFRAAERAFQMITQVSGRAGRKHKKGLVLIQ
                     AIKVDHPVLQYVITNDFHGFYEKEIAERRQFEYPPFTRLIRITFRHSQKERVFATSDW
                     FAAGVRASLGDRLLGPAVPGIPRIRNKYLMELMVKFPNNRQAGDMVKEIIKNQISVMM
                     SDPVHKKVDFLLDVDPM"
     gene            23705..24145
                     /locus_tag="PLL28_01030"
     CDS             23705..24145
                     /locus_tag="PLL28_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNF67922.1"
                     /translation="MLSKKAKYGLKAMFYLAKQFGKGPVLISDLAEEEHIPKKFLELI
                     LLELKKNGLLHSKMGKGGGYYLNKPPGEVTVGQVVRILDGPLAPIPCVSKTAYRKCDE
                     CENEKTCEIRKIMMQVREASATILDHTSLLEAPKALFTVARKNK"
     gene            24252..25292
                     /locus_tag="PLL28_01035"
     CDS             24252..25292
                     /locus_tag="PLL28_01035"
                     /inference="COORDINATES: protein motif:HMM:NF024996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNF67923.1"
                     /translation="MKIKLLMFVTALAFGLSSRAQAPVESYNIKVSQKLELRGLTHIR
                     YQLFDDSTKIDAFDLRRARLDFRGDIAPKMAYRLHLEMANSPKILDATFVYKPYEWLN
                     LNVGQSKTPYCYDNLYSPWTLLTVSRTQIDNALSNRESDLYGNQNGRDLGFWLSGKYS
                     IGEEGNKRPIIDYTLGVYNGSGINVADANQDKDYGAALGVSPIKDLWLFGRYYTGTGR
                     TTLEPETDATKTRFGGNITYKYKDFLFEAEYLSGTDESDSLAQLDRNGYYITLGYTPI
                     KDKLQVIARLDNYDKDANKDLNIVNKYILGASWFFTKNTRVQLEYDIVAEEDADNQIK
                     NNLFAIQFQAAF"
     gene            25398..26369
                     /locus_tag="PLL28_01040"
     CDS             25398..26369
                     /locus_tag="PLL28_01040"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNF67924.1"
                     /translation="MKKAIISVALGMILMVNGAFVNAPVASEHPAELEGKIKISGAFA
                     LYPLAVKWGDEFKKLHPKVSFDIQGGGAGKGMVDVLAGNVDLGMVSREITKEEAGKGA
                     LGFAVCKDAVVATMNANNPYLSKIYETGITRQQFYEIWMGRSITTWGQLLKNGSKEKI
                     SVYTRSDAAGAADSWAKYIGGATKKQDDLEGIGIFGDPGLAQAIASDKLGIGYNNVGY
                     AYDFTTKKPISGIAIVPIDLNENGKLDPEENFYSNADKLNMAVLSGKYPSPPARNLYF
                     VSKGEVTDPVLKEFLKWVLTDGQKYVAESGFVVLPKQTLEEQVAKIK"
     gene            26467..27447
                     /locus_tag="PLL28_01045"
     CDS             26467..27447
                     /locus_tag="PLL28_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNF67925.1"
                     /translation="MKPSKLFLLIAISAITFSSCSGDKKENTEEAGKLEGKISISGAF
                     ALYPMAVQWGEEFKKLHPDVQFDIQGGGAGKGMTDVLSGTVDLGMVSRDVNDEEKSKG
                     AFDFSVCKDAVLPTINAENPYRELLYQKGISNQQFIDVFITGKITTWGQLLGNGAKEK
                     IDLYTRSDAAGAAESWAKFLGGKKQEDLKGVGVMGDPGLAQAVVQSKFSLGFNNVSFA
                     YDNVTKINNPGIAVAPIDVNSNGTLDADENFYGNIDQVNEAILNGKYPSPPARPLLFV
                     SKGNPTDPVVIAFLQYVLTEGQKGVSDAGFVALPADVIKQQIEKLPSAKQ"
     gene            27475..28482
                     /gene="pstC"
                     /locus_tag="PLL28_01050"
     CDS             27475..28482
                     /gene="pstC"
                     /locus_tag="PLL28_01050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNF67926.1"
                     /translation="MPKEINDDDIQNITENIDELEITAVSTQQSTPLVQTGSNPLRVI
                     LDGFMGKVVKAMTIFSLLLLVLIIIGLVLKSIPVLKDNPLSTLLFSSQWSPFKGEFGF
                     LPFILSTVYVTLIAAVIAIPLSLFTAIYLSEYANKRFLKYAAPMLDILGGIPSVIYGI
                     WGVLLIVPFVRDDFSAWLGVDSVSGYSILSGGVVLAVMVFPVVVQIVYEVLKTVPHEL
                     REATLSLGATKWETIKKVVIKRASPGIIAACILGFSRAFGETIAVLMVVGNVVQIPKG
                     IFDEGYPIPALIANNYGEMMSIPMYDSALMFAALILLVIVLVFNVYARWVLNRIEKSI
                     K"
     gene            28488..29336
                     /gene="pstA"
                     /locus_tag="PLL28_01055"
     CDS             28488..29336
                     /gene="pstA"
                     /locus_tag="PLL28_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNF67927.1"
                     /translation="MKKRKIEELIFNIIMRVSAFIIFGSLIAISATILIKGVPAMNLD
                     MITKTPSGGFFLGKEGGILNAIIGSFYLSVGATIFALMICIPVVIYINVYASENSKIV
                     HYTRLSLDILYGIPSIVFGAFGFVLMLFLGMHVSLLAGMITVGLLIIPIIARTMDEVM
                     RQVPVELKEASYSLGATKWETASKVLLKQALPGLATASLLGLGRAIGDAASVMFTTGF
                     TDNIPHSLQDPAATLPLAIFFQLSSPIAEVRDRAYAAALILTVIIFAISLISRILIKR
                     FSKNTL"
     gene            29346..30116
                     /gene="pstB"
                     /locus_tag="PLL28_01060"
     CDS             29346..30116
                     /gene="pstB"
                     /locus_tag="PLL28_01060"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNF67928.1"
                     /translation="MTSSTIKSHISIKDLNMHYGKNHALKNISINIPDKKLTAIIGPS
                     GCGKTTLLKTFNRLIENVDGTNITGEVLVDGENIYGKNADVTNIRKKMGLLNQRPTPL
                     PMSIYDNIAFGMSIHGIKQKGVLNEKVEHFLRVASLWDEVKDRLHSPAARLSIGQQQR
                     LCLARGLAVEPEIILGDEPTSALDPISTKHIEQKFVELKEQYTIVMVTHILRQARRIA
                     DYVIFMYLGEIIEHGPTEMVLGNPQHQLTKDYVGGAIS"
     gene            30299..32062
                     /locus_tag="PLL28_01065"
     CDS             30299..32062
                     /locus_tag="PLL28_01065"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNF67929.1"
                     /translation="MEIAIVLTLLVLAIIAFSTEKISVDIVTMTCVLLLVLSGIITYK
                     EAFAPFGSDFIIMLASIFIVTSAIDSSGVLELWTSKLMRRSAGNKMKLIMPMISITGL
                     FSSLMNNTTVTALMINPAMAIAKKSGINFSKILLPLAFASIVGGTCTLIGTSTNVAVN
                     AYLGKNNFPVLGMWDFTWIGVIMLLVTLLYMVLLGVRMLPDRKNDELTDNYGIRSYLS
                     EIKILPNSSLIGQVIQDSDISRMGFTVLAVIRGDRQFAPGENVSFRENDIVLVKGLMQ
                     QLIGVKNTKGIDIVADTLDFYNEKDNKLRLAEMVVPGRSNLAGFTIAESDFRRKYGFV
                     VVAINREGKNITEQLNNIRFEVGDMLLVQGRNEAFDKLSSHHDLVLLGDHTPPVKNLW
                     KGYFALAFFMLAIVVSSIPAFNVHVSVAFLASAVLSMLVGAVKPEDAYKNIEWKLLVL
                     IAGMTAFGTAMANSGADKYLAGLIVDWFGGMGPMGIMAGFMVLTVLLTQPMSNAAAAL
                     VVLPVALEAAHTLDVNPLTYAVAVMLSASVSLITPFEPSCILVYGPGKYKFGDFFKVG
                     GILTLILMVIIYLTVPITWPL"
     gene            complement(32046..34544)
                     /locus_tag="PLL28_01070"
     CDS             complement(32046..34544)
                     /locus_tag="PLL28_01070"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNF67930.1"
                     /translation="MSYQTPDGLSYEEVLASRKLHGSNVQEVQQRNLLVGIILEIVAE
                     PLFILLSVTMVLYFILGEIQEGLILMVALGIVSGISIFQEQRSRNAVNALKDLTSPRT
                     QVIRNKEQISIHTDEIVVGDIILLEDGNIIPADATILFSRDLTVNESVLTGESLPLYK
                     SADSADNLLFQGTTIVGGSATATVTAIGLNTRLGEIGAKLSDIAVQKTPLQLQIRKFI
                     QSMVIVGVIAFLIVCIVNYLTSKNIIHSLLHGLTLAMSILPEEIPVAFSTFMALGAFH
                     LYKKNVIAKSPYTVETLGAATVICSDKTGTITENLMKPIAIYDAIDDNVHDFNISRPA
                     YHPLIEFAMWASEPEPFDQMEKQIHQLYTEVALSDLRLQFSMVHEYPLDGTPPIMTHV
                     FFNDNEYKIGCKGGLETLLPLCNADKETIQRVTNQAEIFTGKGFRVLAVAKAAFPENG
                     LPARQNLFSFDLLGLIAFYDPPKAKIKDLIQTFYQAGIQFKMITGDHAATAISIADII
                     GMQHNGKILSGNEVMQMSDAALSDAVESTHIYARMFPDAKLRIVEALKAKGEIVAMTG
                     DGVNDAPALKAAHIGVAMGKRGTETAKKAAALILVDDDLMKMADAVALGRRIYENLKK
                     AIRYIISIHVPIILIVTLPLLLGWAFTDIFQPIHVIFLELIMGPTCSIVFENEPIEKG
                     SMQRKPRKMSMNFFSWQELNLSIIQGLAITIACLGLGYYFMQHQMSDPQVRTAIFSTL
                     LFSNVLLTLTNRSFTHSILHTLKYKNKLIPIILSIAMGILVVSLTIEPVRNLFHFEML
                     TFSQLITCLCFAIAGVLWIEIYKILKTVNLTAAR"
     gene            complement(34614..36038)
                     /locus_tag="PLL28_01075"
     CDS             complement(34614..36038)
                     /locus_tag="PLL28_01075"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNF67931.1"
                     /translation="MRFKKKIGILTYIIFDYMSAMVVWAGLFFYRKFVIEDLPFDIDG
                     QVLSDNNFFAGIISIPVIWLGLYFIAGTYTDIYRKSRLAEITRTFVVTFIGVLIIFFA
                     LLLDDYISDYRDYYQIFFVLLFGQFLLTTIVRMIILTRAKRQLQKGVVAYNTIIIGGD
                     QRAYSIYREITHYKKSLGYAFVGYLEANGDHHSDLEGHMPCLGNLSQLESILDQRNVD
                     EVIIAIETSEHHLLNNLIDTVAGKGNIVIKIVPDMYDILSGSVKMSNVLGAVLIEIYP
                     DLMVGWQRMIKRALDVTVASIGFIVLWPLYLFIAIRVKMSSKGPIFYHQERIGLHNKP
                     FTIYKFRSMYIDAEQNGPALSSENDERITPWGRIMRKWRFDELPQIFNILKGEMSLVG
                     PRPERRHYIDQIVKIAPSYKHLTKVKPGLTSWGMVKFGYASNVDEMVQRMKYDLLYIE
                     NMSLAIDFKILFYTLLIIFQGKGK"
     gene            complement(36038..36910)
                     /locus_tag="PLL28_01080"
     CDS             complement(36038..36910)
                     /locus_tag="PLL28_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67932.1"
                     /translation="MLNKTREILIILFAVGLFAGCEIINPEEITPSYVYIDDFTLTTN
                     LETQGYASSKITDGWIYINGTFIGAYELPATVPVLTEGETDIIIYPGIKENGISGVSM
                     IYPFYNAYTITRTLTAGATDTLHPSCTYKPSAGIQFDMLERFESSNAFGGVETEVGLN
                     TTTDPSLVFEGNRSAIANFYPDLDTFRVASSEPIAFPGADKQMFLELDYLAGMDFNVW
                     LKCNTTDQPVYDEVLTITEKDYWNKIYVNLNPSLQFFAQYSPISIQLEFRAVNYDNDT
                     SSIMFDNVKIIQTK"
     gene            complement(36925..37908)
                     /locus_tag="PLL28_01085"
     CDS             complement(36925..37908)
                     /locus_tag="PLL28_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNF67933.1"
                     /translation="MVKIGVLGAGHLGKIHIKLLKEIPTVELIGFYDPSDANAEQVVN
                     QYGVPRFTNMHRLIDACDAIDIVTPTISHYECARAAIKKAKHIFIEKPLANTVSEAKS
                     LVALSHEAHIKAQVGHVERFNPAFLSVKDQHFAPMFIESHRLSTFNPRGTDVSVVLDL
                     MIHDIDIVLHLVKSNVKKVSASGVKVISNQPDIANARLEFDNGCVANLTASRISMKKM
                     RKMRLFQRDSYVSIDFADNVTEILTLSDAKDHPDALTIDINTGDQFSKKAVRLDRPEV
                     QPVNAIKLELEMFANAIIHDTETPVTIEDGFRALQVAHMILQKVEKNAAFH"
     gene            38039..39703
                     /gene="lnt"
                     /locus_tag="PLL28_01090"
     CDS             38039..39703
                     /gene="lnt"
                     /locus_tag="PLL28_01090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNF67934.1"
                     /translation="MQGSINRTLGLFLGLPLLSGVLFWWSWPPMHTAIPIFFAFVPLL
                     FAERLISDSYTKYTGLKVWFALFLGLLVWNLATTWWVQKASVAGGLFANIANPILMTI
                     PLMLMRKVRLKFGKLAGYLAFVVFWMSFEKIHLTWELTWPWLTVGNVFAAQYSWVQWY
                     EITGVFGGTLWVLLINIWVFHAITEYKQPIISATKKKQLIFHALGILIIIALPVLISL
                     QMYRNYQDKGKPVQFTVLQPNYDPYTEKFTIPYRVQMEEMLRTSLQHVNSETDFLIWP
                     ETAIQTDVWLDKLSWQKPVRDVSKAIDSFPNLVCLIGINGYEKYPSADKATATARHLE
                     YASPVTGKPDTIYFDVYNTALALNSEGPIGYYHKSKLVPGVERMPYPEYMKLLNYFTI
                     NLGGIEGSLGIQKDRTVFFNKDSVGIAPVICYESIFGEYVGDYINKGANIICIITNDG
                     WWGDTDGYKQHCMYASLRAIESRRAIARSANTGISCFIDQRGDISQAQPWNVQSAISA
                     SLPANSAFTFYTRHGDYLARFALWLSGGMLVILLIQRLLNKFSKRV"
     gene            39696..40505
                     /locus_tag="PLL28_01095"
     CDS             39696..40505
                     /locus_tag="PLL28_01095"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNF67935.1"
                     /translation="MSDSAENVSLPLEDISHKVMALVKPIGAYMREQRKSFDGVAISK
                     KGIRDFVTEVDMQSERQLVAGLRNIFPDAGFVTEENTVEQIDTYYKWIIDPLDGTMNY
                     VHGIPIYSISIALQAGNKFVLGVVYEVANDEMFYTWQGASSYCNGKTISVSACHTLQD
                     ALIATGFPYIRNDQRTREISNTLKFFLDNGRDIRRLGTAATDLCYVACGRQDVYYEGF
                     LNIWDIAAGILILQNAGGVAVNFKGEENYNEGRIVGTNPNLIGDVLRGISF"
BASE COUNT        11757 a         8097 c         8705 g        12050 t
ORIGIN      
        1 cctttctcct ctttcctctc tcctttctcc tttctccttt ctcctctttc ctttctcctc
       61 tttcctttct cctctttccc taaaatttaa attcccatga aacatttgga ataattggga
      121 acaaactcac ctgcttataa ttattcgtat tcgtcaaata atcatactcc agataaatga
      181 agaagggatt ttttctgcta taagcattgt atgcgccaat attccatgtg cgctcatatt
      241 tttctttctc tttatgaaaa gatacgttta aatccagtcg atgatatgca ttcattctat
      301 atccatttct accttcatag gcataaatcg tctcattaaa catgccttca tatttcgcaa
      361 tcggcaaact tatcggttgc ccggtgctat aaacccatgt tcccgagaaa tctatatggt
      421 cgttgaattt ataaatacat gcaatctcaa aatcattgcg cctgtcgtac ttatatggaa
      481 atttttctcc cagattgatt gttgggaatt gcctgttgct ccatgacaac gtatatccta
      541 tccatcctgt cacttttccg ctgcgctttt gaataaataa ttcagcgcca taggcccagc
      601 cctcacctga ttccacttta tcttcccagc catccaagcc atcaactaaa taattggtgc
      661 catccttgta ttcaatgata tctttcatgt atttataata tccttccaca ctaatctcat
      721 attgttgttt aaatgtgtag gctattccaa ttgcaggttg atatgctttt tgcggaggaa
      781 cactatccgt cgcaggaacc cacagatctg ttggtaaccc gatgccggag tttgataaca
      841 ggtgaatata ttgcgtcatg gtactgaatg atgccttcag cgaaaggtct ggatttaata
      901 aatatctgcc actgatgcgc ggttgtatgc tgccgtaata tttttcattt accagaaatg
      961 ctgatgcgtg taatccgtag ttcactttaa agacatcact tatctcataa tcgtcttcac
     1021 catagatatc aaattcatgt gcaaaaatat ttggtgcgcc aagttcaaga ttgaaatctt
     1081 ctgttccggg ctcattggtt tcaaattgtg tggctccggg acgaaaagta tggtatgtat
     1141 atcctcctcc atattttaaa taatgcatgg ggtttggaat ataatcgacg tcgtatttta
     1201 aactccaatc attgatgcct gaaaaatatt cgaatccaaa atacgtgtcc ggatcatttt
     1261 cgcgctcatc tgtgaaattg ctgaaaatat caaaggtgta atccgtgaaa gttgccgtta
     1321 aattgccaaa agtctttttt gaatacatat gattccatcg tgcaacagca gtggcattac
     1381 cccacgaaat gccactttct tccgaccgat ttgtttccgc atcatattga taatcattat
     1441 gaaaataggc cttatcgcga ccaaaataac cactgacata caatctgtcg cggtctgaaa
     1501 acttgtgatt aatcttggca tttaaatcgt agaaataata gccttgttgc cctgaataat
     1561 cgtcttgtaa tgcaaaaatc ggccttgtga taagatcgat atatgttcgt cttgctgaaa
     1621 ctataaagga ggttttgtct tttataatag gtccttccaa tgttaacctc gatgcaataa
     1681 tgcctatgct cccttcacca tgaaatgttt gcatattgcc ttccttcatt ctgatatcta
     1741 caacacttga taaccttccg ccataccgtg caggaaatcc gcctttaaca agagttacct
     1801 tgttgatggc atctgaatta aatacactga agaatccaaa aagatgtgat gcattataca
     1861 caggcacacc atctaacaaa atcaaattct gatcaggaga accaccgcga acaaataaac
     1921 cgctcgtgcc ctctgaacca cctgaaacac cgggcaataa ttgcagggtt tttaatacat
     1981 ctacttcacc aagaaaggca ggtaatgctt ttatctgttc aatcggcacc tccatcgtac
     2041 tcatctgcgt gcgatcctga agtttgtctt cattgtctgc ggtaactatt acagtctcta
     2101 attccaagtc aggttttaac tgaaagtgta tcaccgtatc tgaaacgagg tgtatatttt
     2161 tagtaatagt gttatacccg atgtaactac aaacaatttg cgcatcaccg ccggatatcg
     2221 tgagactgta aaacccataa ttattagcta tagtaccggc ataatcacgc ttatcgataa
     2281 tggttgcgct gatcaaatgc tcgcccgatg aggcatctgt tacataacca ctaactgtat
     2341 attgctgacc gcggaccaga aatcctaaac atatcagggt cagactcaat attgattttc
     2401 ttaacataat taaaataaat gcgggtgaaa atacgcaaat gccccgattt gccctttcac
     2461 ccgcaggtat atgattgttt tttgacaagt gttaatttga tagcatactt acctgttcaa
     2521 gtattcatcg ttataatcct ccagcacatc attgacatta cattccatct gcttaacggc
     2581 atccagcaat aaagtatttt cactcgggaa tggaatgggc tgaccgccaa ttttttgttt
     2641 cgactgcgca ctgagggctt catagtaccc ggttgcagta gcataataat tttggaacac
     2701 agcatcgctg cgaacaggaa tggagcgcat aactcttccg tttgcatcct tatacaatat
     2761 ctcaccgctc atggtggcga ttttttcctg ccatgtctca gttacaaata cagaaactgt
     2821 tttgtaatca ggcaccttta tcgggttgcc aaggctatct ttaacaatat gaccctgagc
     2881 gtcatacagg aattcaaagc cgtcctgtat ctccttggtt tcttcatagc ggttagtggt
     2941 caccttgtcg gggtatgcct ctatggtgcg caccaccagt tctacagtga aatccgcttc
     3001 tttagggtca taggcattat acagcctgaa ccaattgcca ttgtcctcat acaccttgaa
     3061 acggttgatg gcttgctgta catcgtagtt ataattgcct gaactctggt tggtgagcac
     3121 aaacaatgcc tgagccattc ccttttctct ggctgcggcg atctttgcat ccaaatcttt
     3181 ataggtagag tagtaacgat atattttttc atactcatag taggcatcgc gcgcctgatg
     3241 catatcaccg gatttcatct tttcatcacc aagtgcatac caataggcag cggcgttctt
     3301 tttcgcattg ttaagctctc cgacggcatc cacaaactta aactccgcat cgcgctcctc
     3361 ggtgccaaga tataacggca tcaagggctt tatcttctcc tgacgcacat aaatcccttg
     3421 gtagatgtca taaacggtcg accacctgtc gggattaccg tcggtatgta aagctttaat
     3481 agccgccaga tcaccatcat tggcgcgacg gaacgactct tccaggattt gaatgtactc
     3541 cgccttgtcg ggattgtcga tcaggcgttt tacacaaatg tcgatggcat catcgtaatt
     3601 cccctttctg aattctttct ctgcaacttt gcatccactg gctaacaggg taaataagca
     3661 caatgcgtaa atcattcttt tcataaatcc attgattttc aattactcac atacaaaact
     3721 gtgccagtgt tgtcgcttta ccaaaccttt acgtaaccca caggtatgtg tataactcac
     3781 aatgttaatt tagaacacgt ctaaacaagc tatttcatct tcgtgtaacc cttatgcagc
     3841 aaggatttga gcatgacgaa ataaggtcga aaaaatttta aaagatttgt taaagatttg
     3901 aagcttggtg gttagctttg cacgggtttt tagacaacag ccatatttta accaattaaa
     3961 tatactctat tgtgcgaatg aagaaacaga cgaccatcag acgcctactg accggtttct
     4021 tatttttcgc gtttctgaca acagccaatc aggcgtttgc aggcgaagca gaaggacaaa
     4081 cattgttcca aaccaattgt caacgctgtc acctcatcga taaaaagatg actggaccgg
     4141 cattgatgga cgttcaatcg cgttggaccg acagcaccca cctgtatgca tggatcagaa
     4201 attcacaggc tttcctggct acaggcgata aatatgctaa cgatttgtac aaacagtaca
     4261 acagcatcat gcctgctttc ccgcagttta aggatcagga tattgcagac atcctctact
     4321 acatcaaagt agaaggtgaa cgcaagaagg cagaagcagc caacacaaca accggcggac
     4381 cggttactac cacagcaaag aatgataact ctacccttta ttggatattg atcattgcgc
     4441 tggtatttat tgcattcatc ttggcacgtg ccgttcgtta tctcgatggt ctcgtgcgtc
     4501 agaaatatga cgaaggtgaa gctgatcgca ctccgttctg gcgtcaacgg aaatatcgcc
     4561 cactggttgc attattggtt ttggctttcc tatgtctgct cagcgttgcc atgtggaaca
     4621 gtgctacatc gctcggccgt cagcaaggtt atgctcctga gcaacctatt gcttactcac
     4681 ataaagtaca tgcgggcatc aataagattg actgtaaata ttgtcacgta ggtgccgaac
     4741 gcggaaaatc tgctgtgata ccatcattga gtgtttgtat gaattgtcat tataacattc
     4801 agggagtaac aggaaaagat ccaaactatc cgaaagaagt ttataataaa gagattgcta
     4861 agatctatag ctatatcggt ttcaatcctg aaacaaatac ttacgataaa gaagctaaac
     4921 cgatcgagtg ggtgcgtatt cacaacctgc ctgatcacgt atatttcaac cacgcacagc
     4981 acgtaacagt tgccggcatc gaatgtcaga cctgtcacgg ccaaatccag gaaatggatg
     5041 tggttcaaca acaggaaaac ctgtcgatgg gatggtgcgt gaattgtcac cgtaattcaa
     5101 acgtcaactt tactaccaac gatttctata agccttacga ggtgctgcac gaaaaactta
     5161 aaaacggcga aatcaaggat gttaaagctg tagatatcgg cgctactgaa tgtcagaaat
     5221 gccactatta attttattct aataacccat atctaaccat aaccagatgg cagaaaaaaa
     5281 atactggaaa ggccttgaag agttaaaaca agagccggaa ttcatgcaac ttacgcaaca
     5341 tgaatttcac caggaaatac cggttgagtt cttcgatgaa caaaacgaca cgctgcagtc
     5401 ttcgcgtcgc gacttcctta aaataatggg attcagtctc accgcagcta ccgtagcagc
     5461 gggatgcgaa atgcctgtgc gcaaagtggc accgtatatt ttcaaacctg aggatgtaat
     5521 tccgggtatc gcaacttggt atgcttctac ttattatcac ggcggcgact attgcagtat
     5581 tctcgtgaaa actcgtgaag gtcgcccaat caaagttgaa ggtaaccgcg attctctgat
     5641 cactaaaggt ggtacatcag cacgcgtgca aggttctgtt ctgagccttt acgatactgc
     5701 ccgctaccgc aatccaatgg tgaaaggctc acgtggcatg ggaaatgcta cttgggacga
     5761 agtagataaa gccatcatgg gtaaattaga ggcgaatcca aatgcgcaaa ttcgcatcgt
     5821 tacttctaca gttatcagcc cttctacaaa aaaggtgttt gccaaatttg ctgagaaata
     5881 tcctaatacg aaagtatatg cttacgatgc catttcttat gatggtatgt tgatggcaaa
     5941 catggctaca aaaggtaaag cagaaatacc tcattataat ttttcaaaag caaaagctat
     6001 cgtaggtatc gatgccgatt tcctcggaac atggatatct cctgtagaat ataccaaagg
     6061 gtatgtggca aaccgcaaag tatcgaaagg aaataccgat atgagccgtc actaccaatt
     6121 tgaaagccgt ctgtcactca ccggttctaa tgccgattat cgagtgccgg tactgccctc
     6181 agaattaggt atcgttgcac ttaatttata tgatgccatc accggcggaa atgcttctaa
     6241 tggtgttaaa attcaggatg ccgaaaaaca aaaacgcatt gctgaagctg cagcatatct
     6301 gaaagctaat cgcggcgaat cactcgtagt aagcggatgc aatgataaaa atgtacaagt
     6361 tgtcattaat gctattaacg atgcattagg aaactatggt actaccatca gcttcgacgg
     6421 tgcttcacaa cttcgtcaag gtactgacgc aagcatcaaa acactgatca gcgaattgaa
     6481 tggtaatcaa gttacagccg taatgtttta cgattgtaat cctgtgtatg accacgcaat
     6541 gggtgctgaa ttaaaagaag ctatcggcaa agcagctatt tctgtttctt tcgctactgc
     6601 aatcaacgaa actgctgaag cttgcggaat catttcgcct gatcatcatt atcttgaatc
     6661 atggaatgac ttcgaaccac gtaaaggttt ctattcactc agtcaaccta ctatccataa
     6721 tctgttcgaa acccgtcaga tgcaggattc attgttgaaa tggagtggta ataatattgg
     6781 ttattatgac tttatgatgc aagcctgggc tgaaggtcca ttggctggct ctatatcaac
     6841 taccactgct ttcaacaaag cgttgcagat cggtttatat caaccggaat cacatcgggg
     6901 aatggaaggt gccggcgata tcgtaaaagc aatgctcgct gctgatgcag taatggcgaa
     6961 tgaagcacct gttgccgact tatcttcttc tgcatctgca atcatcgcaa tgtcgaaaaa
     7021 agcttcgggt ttgcaagtgc agttttatga gactgtagct atcggcgacg gtcgttatgc
     7081 cgacaatcca tggttgcatg aaatgccgga tccgatatca aaaatcactt gggataatta
     7141 tatcagcgta tcgccaaaat atgcaagcga aaaaggactt atgcatggcg atatggttac
     7201 attcagcgta aacggtaaat cagtgaaatt acctgtggct attcagccgg gacaggctta
     7261 tggatctgtt tctatcgcgc ttggatatgg ccgcacagct atcggaaaag gtggtgcagg
     7321 tgttggtgtg aatgtttatc ctatgctcga tgcatcaggt gattgtgtta gctatatggc
     7381 atctaatgct acgatggaac ctgtaggtga aaacgtaaaa cttgcacaaa cacaaacgca
     7441 tttcagcatc aacgacggac tttctgttcg ccgtatcatc aaagaaacta cccttgcaga
     7501 atataagaag agtgctaaag ccggtaacga agaccgcgaa gaagcacttg cctggaataa
     7561 tgtaaccttc tatcccgatt atttcggtaa aaagaatggt ttcaactggg gtatgaatat
     7621 cgacctcaac tcttgtatcg gttgcggcgc ctgcgtggtt gcttgtaatg ccgaaaacaa
     7681 tattcctgtt gtaggtaaaa aagaagttat ccgtgcacac gaaatgcatt ggatgcgcat
     7741 cgaccgctac tacggtggcg atccggaaaa tccggaagtg atctaccagc ctatgctgtg
     7801 tcagcactgc gaaaatgctc cttgcgaaaa cgtttgtccc gtgggcgcta ccaaccacag
     7861 ctccgaaggg tttaaccaaa tggcttataa ccgttgtatc ggaacacgtt attgcgcaaa
     7921 caactgtccg tataaagtac gtcgcttcaa ctggttcgat tacggttctg ccgatacttt
     7981 cggaactatg aacgatccaa gcggaacaga aaacctcgga ttgtttgaag acctcacccg
     8041 tctggtattg aatcctgatg taacagttcg ctcacgtggt gttatcgaaa aatgctcatt
     8101 ctgcgtgcaa cgtattcagg atgcgaaact taccgctaaa aaagaaggtc gcgaattggc
     8161 cgacggtgaa atccgcactg catgtcaaag cggttgccct gccgatgcta tcgtattcgg
     8221 taacaactac gatgagaaga gtgcaattac aacagaaact atggatgaaa gaaccttcgg
     8281 tattctcgaa gaattccact ggttaccatc tgtaacatat aaaactaagg taaggaacaa
     8341 agacaaagcc gacagtcacg gtactgaagc gctcgatatc atggatttgt tcaattataa
     8401 ataattcaac cacttaagca gttatgttcg tatcaccttt aagggaaccg ctgatccaag
     8461 gtcataagac ctacaaacag atatctgatg atatttgtgc gccggtagaa gccaagccga
     8521 gcaaggcctg gtttattgcc accaccttgt cgggactcct cgccatcaat ggctttgtga
     8581 tgatcggcct taccattact tacggtattg gtatgtgggg attaaacaaa accgttggtt
     8641 gggcctggga tatcaccaac ttcgtttggt ggatcggtat cggtcacgcc ggaaccctga
     8701 tctcagccat cctcctgttg ttcagacaaa aatggcgcac gggagtgaac cgctcagcag
     8761 aggcgatgac tatcttcgcg gtaatctgcg cgggcttgtt ccctatcatt cacatgggac
     8821 gtgtatggct cgctttcttc atcttccctt atccaaatac acgcggtccg gtatgggtga
     8881 actttaactc accattgctt tgggacgtat tcgcgatttc gacatacttt acagtatctc
     8941 tggtattctg gtataccggt ttgattccgg atcttgcttt gatccgcgac cgtgcgaaat
     9001 cggcattcac caaaaagatg tataacatcg cgtgcttcgg gtggaaagga acagctaaaa
     9061 actggcagcg tttcgaatcg atttcactgg ttctcgccgg tttatcggct cctctcgtat
     9121 tgtcggtgca caccatcgta tccttcgact tcgccacatc ggtaattccg ggatggcaca
     9181 ctaccatctt ccctccatac ttcgttgccg gtgcgatctt ctccggattt gcgatggtat
     9241 tgacgctgat gttggtggta agaaaagtat ttaatcttca ggaatatatc actctcgccc
     9301 atatcgaggc gatgaataaa attatcatcc tcacgggatc tattgtgggt gttgcctata
     9361 tcaccgagtt atttactgcc tggtatagcg gtaacccata cgaacgttat gcattcctca
     9421 accgtgcaat gggtccttat tggtggagct attggggtat gatgacatgt aacgttgtat
     9481 caccacaatt attctggttc cgcaaattac gtcgcagtgt agtattcaca ttcttcatgt
     9541 cgatcttcat caacgtgggt atgtggttcg agcgtttcgt gatcatcgtt acgtctacac
     9601 accgcgattt tattccttct aactggacaa tgtattatcc tacttatgta gagatgttca
     9661 tttatatcgg cacgatcgga ttctttatga cactgttcct catcttctct aaagtagcac
     9721 cggtgatagc agtagcagag gtgaaatcga tcctgaaaac agcgggcgat caatatacag
     9781 gccctgatgc cattcataaa cacgaagaag ataatcacca ttaatccaat aacccagata
     9841 aataaaggtt agcaaatggc cactaaaaaa ataatgtacg gtttatatga tgatgaggta
     9901 gtactcatca aagccgtaaa aaaagtgcga gcagaaggat tgaaaataca cgattgctat
     9961 acaccatttc cggtgcacgg acttgatgat gcaatgggct tgcgtatgac aaaattgcac
    10021 agcgccggtt ttattttcgg tgctacaggt acgctgacgg ctttgtcgtt catcctttgg
    10081 ataactacca ataattaccc tatcaattac ggtggtaaac catacttgtc gcttccatca
    10141 tggattccta ttactttcga gcttacagta ttgtttgcat cagtaggtat gttccttaca
    10201 tacctctatt tgaacagact tgctccggga atgaaagcag agatcgttga tgaacgcact
    10261 acctgccaca tgtttgcaat gactttcgaa attgacgacc atactaccga tgccaagaaa
    10321 actgaaatca gaaaagtatt gagcgatagc ggtgctgtgg aaatccatga aaaagaactt
    10381 taatacagaa aatccatacc aatggtgaaa agaaacataa tgatcgctct tgttgctgct
    10441 gtgatgattc cggcagtgct cacttcttgt aaaaaagcaa gcggtgatta tccgggcgac
    10501 tcctatactt gggatatgag ttattctcgt gcttacgaac catataccct gaaccctgaa
    10561 ttgcgtgata gcatgaccgc tatgactcct gttgcaggta ctgttcctta tgtaggtaat
    10621 gcagttgccg gtgtgaaagc cgacagcttg aaaatggcac tcaacctgcc atataccatt
    10681 ccaaatacac cggaaggtta tttgcgcgca gctaccgaaa atcacaaccc ttttagtatt
    10741 aatgacgaaa aggttgtagc acagggacaa catttcttta atatctattg cgctgtttgt
    10801 cacggtgttg aaggaaacgg aaaaggattt attgttacag aaggaaaata tacagccatg
    10861 ccaccttcat atttcgatcc gatgtatatg gcgatgacag aaggcacgat gttccattct
    10921 gtaacgtatg gacgtaacgc aatgcaatca tatgcatatg ctttatcgaa agaagagcgc
    10981 tggaaagtga ttacttatgt gaagaaaatg caagcagatt atgccgctca aaatgctgca
    11041 actgctgcaa caacaaccgg cactaattaa cagattacaa ccactaacgt aaaaaaagga
    11101 aatcagaata gcatgaacgg ctcaaataaa tttgaataca cggccaagct gaagaatcgc
    11161 ttcttaggca tgctcgtgat cggagtagta ctcgtcgttg tgggcatgtt aaccggtgcc
    11221 tccatggaca gattctggtc gaattatctg caaaacactt tattcttttt aggaatatcg
    11281 gttttagcag tattcttcct cgctgctcac cagatcggta tggccggctg gcatatcact
    11341 gtgaaaagaa tacctgaagc aatgagccag ttctcgaaag tgggtgctgt attaatgctc
    11401 gtggtagttt tcggagtgat cttcgattat catcatttat accaccattg gacaaatacc
    11461 tttattcagg aaaaagaagt ttctgttgaa gcattgaaag attacgaagc agaacttgcc
    11521 gaagaaggtc atagcactga gcatgccgtt gattacaatc gcacctggca ggattttcaa
    11581 tatcatcagg tagctcctgc tcctgaacat gttgatgcag caaaaccgga acctgaagtg
    11641 ttggaaaatg gaatggttgt aaatccatat tacgatcgtc tgatcgatca taaatctgct
    11701 tatctgaata aaacattctg gagtatacgt tctatcagct atttgttttt gtgggtgtta
    11761 atggcaatga ttattcgccg ttattcattg cgcgaagacc ttgataattc agatactttt
    11821 tatcagaaaa cgcgcaaagc tgctgccgtg ttcctcatca tttgggctgt gagcagttct
    11881 acaatgacat gggactgggt gatggcactc gatccgcatt ggtatagcac cttgtttggt
    11941 tggtataact tcatctctct ttttgttgca agtatttgta ctaccattct tattcttatt
    12001 tatctcaagc gcaatggtta cctgacacaa ttgaatgaga accacttgca tgatttaggt
    12061 aaatatgcat tcggcttcag cgtgttctgg acatacttgt ggttcagcca attcatgtta
    12121 tactggtatg gcaacattcc tgaagaaaca cgttggtgtc tcgaccgcgc acgcacagac
    12181 tacaaatggt tgttctatgc gaacctctta ctcaatttcc ttttcccatt cctgacatta
    12241 atgcgtcgtg atgcgaaacg taatatgaat gtgctgatga ttgtagcaat cggactcatc
    12301 attacccact ggttggattt ttacatgatg attacacctg caactttagg cacagaagtg
    12361 cactttggaa ttctggagat cggaatgttt atcgcttatg ccggcctctt cttgtttgtg
    12421 gtattcggtg agcttgcgaa agcatcactc attccgcaaa atcacccgat gtataaggaa
    12481 agtcttgacc atcatatcta aaatcaaatt atcacaatgg ctaccggttg gttgatattt
    12541 attgcaattt taatgatcgt tgtggtcttg ttccaggtaa caagaacact cgacctagtt
    12601 ggacaacttc gcggaggtga tactaccacc gtaagtatga ccaaatttca tgcttcactc
    12661 ggaatgatat ttatggtgtt gggtatgatc ggatttttct ggtcattcgg acatttccat
    12721 gattatcttg tagatcacca ctcatctgat aacggtctta ttattcatag gatgttcgta
    12781 atcacattgt gggtgacagg aattgtattc gtgatcacga atgtgttgtt atttattttc
    12841 gtttatcaat atcgccaccg tcccggtcat caggctacgc actttgcgca tagcaataag
    12901 cttgagttta tctggaccgt tgtgcctaca attgttttga caggacttgt ggtttttggt
    12961 ttgcaagctt ggaccgatat catgggtgat gctgaggacg atgctattgt gtttgaagta
    13021 accggtcagc aattcttttg gagtgcacgc tatcccggac ctgatggaaa attaggacct
    13081 cgcgataatg atttgatctg tgcagataat ccgcttggca ttgttacccg cgaatggatt
    13141 gaccatcgtc gtgtgacact catcggtagc actaaacttg gtgtgaaagg tgaaattgaa
    13201 aaattgcaag atcgcgaaaa agagatcaat gctattattg cttcttatga gcaacgtatc
    13261 gcaacatcac caaataaata taaagttgcc gatgcgaaag acagtctcga aatcgtgaaa
    13321 aaagaattag gaaaaattcc gggcaagatc gagatacgcg aaaaggctct taccaggatt
    13381 aataaaaaat atacggaaga atatatcaat caacatgcag cggaatttac ttggggatat
    13441 gatgatccaa tgccaagtga agtgcatgtg ccggtcgaca gacaagcatt tgtgaaaatc
    13501 acggctcttg atgtattgca caacttctat attccgcaca tggatgtgaa gatggatgca
    13561 gttccgggta tgccaacatc attcaaattc aagccactca ttacaactgc cgacaagcga
    13621 attgaaatgt ctgaaaatcc attgtggcag gtaacaaaag aaggcgaaaa agagcctcgc
    13681 tggaaaaatt tcaattatga gatagcatgt gccgaattat gtggttccgg tcacagcgca
    13741 atgaaatata cacttgttgt agaaacacaa gaagattacg acaaatggat atccgcacaa
    13801 caaagtaaat ggagtcaggt agcagcttca ttgaaactgg aaaatggttt ttcaaaattt
    13861 gctccgaaac ttgaagctcc tgctgcagaa acacctgcag cacctgctga tacaacaatg
    13921 attacaaaaa aataattgac agaaaaataa attggtatgt cagaacatca cgcagtagcg
    13981 catcacaccg aagcccatgc acacggtcat gatcatcacg gtcacgacgg acatcatgag
    14041 ctgggtttta ttcgcaagta cattttcagc gaagaccata aaactatcgc caaacaattt
    14101 ttggtgacag gtattttctg ggccataatc ggtgccttgc tttcggtatt gttccgtatt
    14161 cagctcggat atccgaatgc tgatcttacc tggatgaaac cattcctcgg aaaatggatc
    14221 accgaagaag gacagatcgc tcctgaattt tataacgcgt tgatcaccat gcacggtact
    14281 attctggtgt tcttcgtact tactgcagga ttgagcggaa cattttcaaa cttcctcatt
    14341 ccattgatgg taggtgcgcg cgatatggct tccggattta tcaatatgct ttcatattgg
    14401 ttcttcttta tctccggtgc tgtgatgttt gtatcattct tcctggctac cggtcctgca
    14461 tcaggtggtt ggacagctta tccgccgtta aatgccttac atgatgcctc ttcaggtggt
    14521 gtgttagggg tagattattg gttgattagt atggcattat ttattgtatc tgcccttctc
    14581 ggaggtttga actatatcac tacagtaatc aacttgcgca caaaaggtat gaagatgttc
    14641 cgtcttccat tgcctatctg ggccttcttc cttactgcgg taatcggtgt attgtcattc
    14701 cctgtgttat tgtcggcagc aatcttgttg ttattcgaca ggaaccttgg aacaagcttc
    14761 tatcttgatg ctattaacct ggcaggtgaa ccattggcga attccggtgg tagcccaatc
    14821 ttatatcagc acttattctg gttcctcgga caccctgagg tatatatcat tatcctccct
    14881 gcgatgggta ttgtttcgga agtgctttca gtgcattcac gcaaaccgat tttcggatat
    14941 accgcgatga tttattcatt gttggcaatt actgtattgt cattcatcgt gtgggcacac
    15001 cacatgttca tgacaggtat gaatccattc ataggttcaa tcttcgtgat ctttactttg
    15061 atcattgccg ttccttcggc gatcaaagta tttaactggc ttacaacgtt gtggaagggc
    15121 gatattcgac ttaatccgca aatgctcttt gcgataggat ttgtttcgtt atttatttcc
    15181 ggtggtctga caggtatctt cttaggtaac tcttccatcg atattcgttt gcacgatacc
    15241 tatttcgtag ttgcccactt ccacttagtg atgggtgtgg ctgcattctt tggaatgttt
    15301 gccggtgtat atcactggtt cccgaaaatg tttggtagat atttgaaccc gacattggga
    15361 tatatccact tctggggaac gatctcagcc gcatatttaa tcttctaccc aatgcacttc
    15421 gaaatttcat tgcctcgtcg ttattataca cactcaggct ttgagacatt cagcgatttc
    15481 gcacatttga atgcattcat ttcaacggtt gccatcattt catttgcatt gcaattgttg
    15541 tttgtattca atttcttcta tagcatctgg tatggtcgca aattgcgtga atcaaatcca
    15601 tacggcgcaa atacattgga atggactaca ccattgcgtc cgggacacgg taactgggaa
    15661 ggagatattc ctgaggttca ccgttggcct tatgactata gcaagcatgg cagagatttt
    15721 attcctcaaa cagaaccatt gtcgcaatat gaaatagaac atggcgaaca tggcgatgaa
    15781 ggtgataggg atatcattaa aaaagaagaa gacgaaaatc acaaaggata atttataaat
    15841 gcgtaaagat tcgtccatag caggatccgg cataggcaaa atttttgctg cgcgactgaa
    15901 agactaccac atgttggtga agttcagatt gagcttcatc gtggtattca gctcgggtat
    15961 cggatattta ttctgcggtg gcgaacaatc gcaattcttt ctattcttgt taggcggatt
    16021 tctcattacg gcagcatcaa atgccatcaa ccagatcata gaatcagata ctgatcagtt
    16081 gatgacacgt actgccgacc gaccactggc tgcaggaaga atggcacata ccgaagccat
    16141 catagcagca ggtgtaatgg cggtatcggg aatcgcttta ttgtggtggc agtttaatag
    16201 tctcagcgcc ttgcttggtg cactttcact catcagctat gcatttattt atacgccgat
    16261 gaagaaggtt tctgcaattg cagtatttat tggggccttt ccgggagcaa tgccaccact
    16321 gattggcggt gctgctgcaa atggagagat ttccaatatc gcgatattat tattttgtgt
    16381 gcagtttgta tggcagttcc cgcatttttg gagcattgca tgggtggcat gtgaagatta
    16441 tgcgaaagca ggattccatt tgttgcctac agctagcgga agatcaaaag catcagcagt
    16501 gcatattttg ttgtatgccg cactcttgat tcctgtttcc ttgttgacct atttcctggg
    16561 ttataccgga acagtatctg cagtaatagt agctgtaaca ggattgcttt tctttttgca
    16621 ggcagtgcat ttatttcgca cagcagacac taaagcagca cgcagtttaa tgtttggatc
    16681 atttttgtat ttaccgatag tattaattgc attattggtt gatcagatat aaataaacat
    16741 ggcagtatca atacataaac gcttcatcgt tcatccatat aaattcaata tgtggattgc
    16801 tatcgtttcg atggtgatga tttttgcagc gtttaccagt gcctatgtgg tgaaaaaagg
    16861 tgatacaaaa acctgggcgg atatacaatt gccgcaaatt ttcaccttgt caactattgt
    16921 aattgttatc agcagtttat tgatgcacgc tgcttatgtt tcctttaaaa aaggagcatt
    16981 ggaatggtat cgcggattta tgacgcttac ttttttggct ggtgccgcat ttttaagtat
    17041 gcaaattcaa ggttggtata ctcttgtagc acagggcgtt tatcttaccg ataatgttgc
    17101 aggaagtttt atttatgtaa tttcaggagc gcattttttg catgtggcag gaggggtgat
    17161 cgcactactg gtgttcagta ttcgttcatt cacccgctat caggcatcga atatagaagc
    17221 aatgaaggat actaccatta atgtcgaagt aatggccacc tattggcatt tcgtagatat
    17281 tttatgggta taccttttct ttttctttat gttaaaccga taaaaaccag atattttgga
    17341 agcagtcgca acaacaaatg caaaagacct ttggtcaggt ggtgactccc ccttcaaggt
    17401 gagttatggc aagatcatga tgtggtattt cctcatcagc gatactttca ccttcgccgg
    17461 tctgttgatc tcttacggag cgatcagaac tagtcagcct aaacatgatg ccgctaaccc
    17521 gctatcatgg cctgatccga acgaagtgtt taacgcaggt cccggatttc acaatgcacc
    17581 gctgatcttc gttactttca tgacgttcac actgatctta agttcagtaa cgatggtacg
    17641 tgccgtgcag gaaggtcatc agatgaatag gagtggagtg ttgaaatgga tggcatttac
    17701 cattcttggt ggtttggtat tcgttggcag ccaggcatgg gaatggtcgc atcttattga
    17761 agaaggaatg acaccacatg gcaacccatt cggtgcatct tcatttggtg ccttgttctt
    17821 cctgatcacc ggatttcacg gtttccacgt aagtgttggt gtacttctta actcatgggc
    17881 atttatttct acataccgcg gagtatttga tcgccgaggc cactatgaaa tgatcgaaaa
    17941 actcggtctc tactggcact ttgtggatct ggtatgggtt tatgtgttct tagcattcta
    18001 tctattctaa acaaacgata ttcagcaata tgtctacttt aactcaagaa gaatcaagaa
    18061 aacgcgtatg gcgagtgtgt ggaattttgg ctgtagttac tgcagttgaa atcgccgcag
    18121 cattgatcca ttacaactac atgcacgact ctcctaagtc gttcctgaat attttgttcg
    18181 ttgtactcag cgctgccaaa gcctattata tcatgagcga attcatgcac cttaagtatg
    18241 aattaaaacc tatggtaatc agtatcctgg caccgttcct cttcctcgtt tggggtatta
    18301 tcgcattcct ctgggaagga cactactggc tgagcgaaaa ggaactttgg catatactga
    18361 aataatatgt cttcaaccag agggcgtata tggaccatcg tgatgctggt catcattctg
    18421 gtgttgccaa gtatcgccta tttaatgtta cgtgaaggtc tgcatgtcag ggaatcagcc
    18481 cctgcaagtg atcatttgat caaggagaat cttcaatcca ttcccaccta ttccggggtg
    18541 tcgtttagag gcgataccat ttctaaaagt aggatgaaag ggaaggtttg tgtactgaat
    18601 tttactacac atgcctgcga aaaatcgatg gatgataaca tgcgcaagct gtttgaaatt
    18661 caggaagatt attacggaaa aacaatttct ttccgcatta tcactaatac cttagatccc
    18721 gataccgacg gcctggataa tatgcgtctg atgagtgagc gctatgcagg acgcgaaatc
    18781 tggcatttta tcaccgatcc cgatagctca ggtgttaaat tatatgattg gtgcaaaaaa
    18841 aatgcaacag atagcacctt aaatgaaact gatacgcagt gtccgcagtt agtatacctg
    18901 gtcgatggca atggcaaact cagaggtgta tacaatattc tcgaagaaca gcaattccac
    18961 gacttgtata acgacatttt gtacttggtg aatcaaatgg aactgcatga gcaaaagaaa
    19021 taatctcatc atttttggag tcagcggcgt agcatttatt ctattcgtca tctttgttta
    19081 cctgccatat gctaaaaagc aggataatct caataaggta aaagaaagtt atcagcatag
    19141 ctcatatctg aaagatcctg tggggcaatt gccggtattt agttgtatcg atcagaatgg
    19201 gaaggtattt accaacgaca gtctgaaggg agatgtggtg gttgcaaatt tcttttatac
    19261 aacgtgtgaa ggctattgtc cgacaactac caaacacctg gctacggtgc aatcatcatt
    19321 gaataaaaat attccttttc acattatatc attcagtctt aatcctgcaa atgataccgt
    19381 agcagcattg cgcaaatatg ctgatatgta taaggctgat gataatgtat ggcgttttct
    19441 tcacggcgat caggaagaaa tttatcagtt aggtgaacaa ggcctgatga cgattgttaa
    19501 ggacacagcg ggcaattttg aatctcattc cgacaggttt gtattgatgg ataagatggg
    19561 taaaatcaga ggtttttatc ggggaacgga ttcacttgaa ttgcaggcac tgattcagga
    19621 tatcaattat ttggtattta aaggagagac taatgaatga acgtacaata aataggtgga
    19681 ttgtagccat ttcaatattg attccggtag cggttaccat tcttttttat atgcctgcgg
    19741 tgaagttgaa tatcgatact tccatattgc cggcctttca tgcaatgctc aattccggag
    19801 tagcaatttt acttttactt ggattgtact tcattcgcac cggaaaaaga ggggcacaca
    19861 gaacgtcaat gcttggtgca ttttcattgt cggcattatt tctgatttca tacgtgctct
    19921 atcatctcac atcagactcc accttgtttg gcgatttaga tcataatggc attgtttcag
    19981 atgccgaaaa ggctgaagcc ggagccatgc gtggggtata tctggtgatt ttattgtcgc
    20041 acatattcct ggcagtgctg atcatgccat ttatcctggt tactttgtca agggcgctga
    20101 cagccaggtt cgataaacat cgaaaaatcg ctcgaatcac ctggcccctt tggctttatg
    20161 tggcgattac gggagtggta gtatatttta tgattgcgcc ttattatccg taaccaaaat
    20221 agctgtaaat ttgccttatg aagtatcgct tgttgattat gttagtgctt gtggtgctgt
    20281 tgtcagcgct gcataccgac cttcaagctc agtgtgccat gtgtaaggct actgcggaag
    20341 aaggattgcg cgaagggaat acccaaagtg ccaatctgaa tgcaggtatt atgtatctgt
    20401 tggcaatgcc ttatctggcc ttcggagtaa ttggattttt atggtatcgc aattataagc
    20461 gtaaaaaagc acaggaagct gccggtctct gatcagagat ctcccaattg tggtcttatt
    20521 agaatttctt ctacaactga cctgtcggaa agttggtaag cctgtaatat taaggtagca
    20581 atgtcatcgg caggaataaa tcttgattcc ggcaatgggg tgccatccca ggaaggtgtt
    20641 aaaactgcac ccggaagcac agcggtaacg cgaatattat cggtttttaa ttcgtggcga
    20701 agattcttag taaaaccaag taaggcgaat tttgaaatag catagctacc accgccatca
    20761 taggaattga tactggcaac agagcagata ttaaaaatgt atcccttttt tgcagtgcgc
    20821 ataacaggga cgatttgtct tgtaagatga tatgcactat atacatttgt ttcgatgaga
    20881 aattcaagcg tgccctcggc ttcttcggtg atattacccg gtagaaatac tccggcgttg
    20941 ttgatgagca catcaataga accgtatgct ttaaggcaat tttcgccaaa cgaatgcaat
    21001 tgcgctctgt ctcgcacatc gcaggaaatt gccaaatgtt tacctgtcgg attggcatcg
    21061 tgtaattgtg ctgacatttc attgagggct gctttatcgc gtgcacaaat ggcaacgtta
    21121 aatccatttt tagcaaataa taatgcacag gctttgccaa taccctttgt tccaccggtt
    21181 atgatacaat tcatctttgc gaaatagtta catttacaaa gtaaaacaat ttgacccaat
    21241 gcgtagtgtg tttgttgatg tgatcttgcc gtttgcactg gagaagaatt atacctatgt
    21301 ggttcccgac gagtttgtag gggtgctttc gcccggcatg cgcgtggaag tgccgtttcg
    21361 caacaaaacg tatacgggaa tagttgctaa ggtgcatgat atcagtccgc aaggatattc
    21421 ccccaaaccc gtgctctcat tgcccgatca tttacaggta gtaaataaaa cgcaacttga
    21481 tctgtggaca tggatatcga aatattatat gtgttcggtt ggcgatgtga tgaatgcagc
    21541 attgccggca ccatataaat tgagtagtga aacagttgtt gtctacaatc cgcaacatgg
    21601 cattgatctg cttacattgg acgaaaaaga atatctgata gctgaggcat tgacattaca
    21661 acaggaattg cgacttattg atgtgcaaca gatattgcga caaaaaactg ttcagccgat
    21721 aattaaaggg ctaattgcga aaggtattgc atttattaaa gaagaattaa aggaatcata
    21781 tactgtaaag actgaaaaat atatcattct gacaccgcaa tatagatcag aagatgcctt
    21841 gcaacaatta tttgaatcac tgcaaaaaag tgaaaagcaa acaaatgttt taatgattta
    21901 ttaccagctt tctgcaaaag agggaaaggt gaagaaatca gttttactca ggcgatcggg
    21961 tgttacggcg gcggtgttga agaccatgat taagaatgac atttttgagg aggtagaaga
    22021 gcaggtgagt cgtttgcaag cttatggaaa tgcagatatg catgacgcgc acttgaatgt
    22081 ggatcagcag aaagcattgt cggagataga ggagcaattt gaggaaaagc aaactgttct
    22141 cttacatgga gtgacaggca gtggtaaaac cgaagtgtat attgaattaa tgctcaagac
    22201 aattgctgca ggaaaacaag tattgtattt attacctgaa attgcactca cggcacaaat
    22261 tgtgacgcga ttgaaaaagc gattcgggaa tttaattggt gtttatcatt caaaattcaa
    22321 tcagaatgaa cggattgaaa tatggcagca tgtattgaat aatgaattca aagtaatatt
    22381 gggtgcgagg tctgcattgt ttttgccatt caatgaaatt ggtttaatta tagtggatga
    22441 agagcatgat tattcatata aacagcacga gccgagtcct cggtaccatg ccagagatac
    22501 tgctatttat ttagggcatt tgcatcaggc aaaagtgtta ttgggaacag caacaccttc
    22561 cgtggaaaca tattatcagg cgaaatcagg gaaattcggg ttggtggaaa tgtttcgacg
    22621 tcatggcgat atgcccatgc caacgataca gatcgtggat atgaaggcag aggagaaaca
    22681 aaaaaccgtg caaggacatt tcagcagtgt gttggttgga aatatcaagc atgcgctgca
    22741 gttgaaggag caggttattt tattccagaa caggagaggg tatgcgccat ttatgatttg
    22801 tcagtcatgt ggctggacac caaagtgtac gcaatgcgat gtgaatctgg tgtatcataa
    22861 atatgccaat gaattgcgct gtcattattg taaccatgtt gaaaatacgt attcagtctg
    22921 ccctgcgtgt aatagttcgc aattaattat taaaggcttt ggcacagagc gcatcgatga
    22981 tgatttacag tatacatttc ctgatgcgcg aactgcacga cttgatttgg atgccataaa
    23041 aaccaagcat ggtcatgaaa aattggtaag atcttttgaa gagggtgaga tagacatatt
    23101 aacaggcact cagatggtga ccaaaggctt ggatttcgat catgtgaatt tggttggcat
    23161 attgagtgcg gatcaaatta tcaatttttc ggacttcaga gcagccgaaa gggcatttca
    23221 gatgatcacg caagtgagtg gaagggcagg gcggaagcat aaaaagggtt tagtattgat
    23281 tcaggcgata aaagttgatc atccggtatt gcaatatgtg ataaccaatg atttccatgg
    23341 attttatgag aaggaaatcg ctgagcgaag acaattcgag tatccgccgt ttaccaggct
    23401 tataaggatt acctttagac atagtcaaaa ggagcgggta tttgcaacga gcgattggtt
    23461 tgcggccgga gtaagagcct cattgggtga tagattgcta ggacctgcgg tgccgggtat
    23521 tcctcgcatc aggaataagt atttgatgga gctcatggtg aagttcccaa acaacaggca
    23581 ggcgggtgat atggtcaagg aaatcatcaa aaatcagata tccgtcatga tgtccgaccc
    23641 tgtgcataag aaggtagatt ttctgctgga tgttgaccct atgtgacgta tttttaccct
    23701 agttatgtta tcgaaaaaag cgaagtatgg gctcaaggcc atgttttatc ttgcaaagca
    23761 gtttggcaag ggcccggtac tgatttctga tcttgccgaa gaagagcata ttcctaaaaa
    23821 attccttgag ctcatattgt tggagttgaa gaaaaatggg ttgttgcaca gtaaaatggg
    23881 taaagggggc ggatactatc tgaacaagcc tccgggtgaa gtaactgtag ggcaggtagt
    23941 gcgcatatta gatggaccat tggcgccaat tccatgtgta agtaaaacgg cgtatcgcaa
    24001 atgtgatgag tgtgagaatg aaaaaacctg tgagatccgg aagattatga tgcaggttcg
    24061 tgaggcatct gccacaatat tggatcatac cagtctttta gaagcaccaa aagcgctatt
    24121 caccgttgcg cgaaaaaaca aataaacata ttgcagatcg ggcgattaca atttataaaa
    24181 ataaatatgt ttataaaaac tactaaaatg atagattata tagatatttg ccctcgacaa
    24241 ataacacatc tatgaaaatt aaactactaa tgtttgtaac agcgctggct tttggattga
    24301 gttctagagc gcaggcaccg gttgaatcgt ataacatcaa ggtatctcag aaacttgaat
    24361 tgcgaggatt gactcatatt cgctatcaat tatttgatga ttcgacaaaa atcgatgcat
    24421 ttgatttacg tcgcgcacga ttggattttc gtggtgatat agcgccaaaa atggcctatc
    24481 gtttgcatct cgaaatggct aactcaccga aaatacttga tgccacattt gtgtacaaac
    24541 catatgaatg gttgaatttg aatgtcggcc aaagtaagac accatattgt tatgataact
    24601 tatacagccc atggacttta ttaactgtaa gtcgcacgca aattgacaac gcattgtcga
    24661 atcgagagtc tgacttgtat ggcaatcaaa acggtcgtga tcttggtttt tggttaagcg
    24721 gtaagtacag cattggagaa gagggaaata agagaccgat tatcgactat acattaggcg
    24781 tttacaatgg ttccggaata aatgttgcgg atgcgaatca ggataaagat tacggtgctg
    24841 cattaggtgt ttctcctatt aaagatctgt ggttgtttgg tagatattat accggaaccg
    24901 gcaggacaac actagagccg gagacagatg cgactaaaac cagatttggt ggcaatataa
    24961 cctacaaata caaagatttt ttgtttgagg ctgagtattt gagtggaacg gatgaaagtg
    25021 atagccttgc gcaattagat cgcaatggat attacattac gttgggatat acaccaatta
    25081 aggacaaatt gcaggtaatc gcgcgtcttg acaattatga taaggatgct aacaaagatc
    25141 tgaatattgt aaataaatat atacttggag cgagttggtt ttttacaaaa aacactcggg
    25201 ttcagcttga atatgacata gttgccgaag aggatgctga taatcaaata aaaaataatt
    25261 tgtttgctat tcagtttcaa gcggcattct aaatagctaa tgatgtgtcc ataaaaagtg
    25321 ctgaatttga tcagcacttt ttatggatac aaagtactac aaaaacgata gaataaatag
    25381 tacataaaaa aattcaaatg aaaaaagcaa ttataagtgt agcactcgga atgatcttaa
    25441 tggtgaatgg agcctttgta aatgcaccgg tagcatctga gcatcctgct gaattggaag
    25501 gtaaaatcaa gattagcgga gccttcgcgc tttatccctt ggcagtaaaa tggggtgatg
    25561 agtttaagaa actgcatcct aaggtaagct tcgatattca aggcggggga gcaggtaaag
    25621 gaatggtaga tgttttagca ggaaatgttg atttaggtat ggtgagtcgc gaaatcacaa
    25681 aggaagaagc gggtaaaggt gcccttggct ttgcagtgtg taaagatgcc gttgtggcaa
    25741 ccatgaacgc caataatcca tacttgagta aaatctatga aacaggtatt acacgccagc
    25801 aattctacga aatatggatg gggcgcagta ttaccacctg ggggcaatta ttaaaaaatg
    25861 gcagtaaaga aaagatttct gtttatacca gaagcgatgc agccggagcc gctgattcat
    25921 gggcaaagta tatcgggggt gctactaaaa agcaggatga ccttgaaggc attggtattt
    25981 tcggagatcc tggtttagca caggctattg ccagtgataa gctaggaatt ggatacaaca
    26041 atgttggata tgcctatgat tttactacta agaaaccaat aagtggtatt gctattgttc
    26101 caattgatct gaatgagaat ggaaaattgg accctgagga aaatttttat tcgaatgccg
    26161 acaaattaaa catggctgtt ttgagcggaa agtatccatc accaccggct agaaatttat
    26221 attttgtttc aaaaggcgaa gtaacagatc ctgtattaaa ggagttttta aaatgggtgc
    26281 tcaccgatgg gcagaagtat gtagctgaaa gtggatttgt agtgttgcct aagcaaacac
    26341 ttgaagaaca agttgcaaaa attaaataaa ctgacgtatt ggttaaggtc aactataaca
    26401 ctatcagcct gctttatagc aggtttgata gtgcttttat aaaaaaacaa aacaacaata
    26461 aagtatatga aaccatctaa attgttttta ttgattgcca tttcagcaat tacattttct
    26521 tcctgctccg gtgataaaaa ggaaaacact gaagaagccg ggaagcttga agggaaaatt
    26581 tcgatttccg gagcctttgc attataccct atggcggtgc aatggggaga agaatttaaa
    26641 aaattacacc ccgatgttca attcgacatt caaggtgggg gagccggcaa aggtatgact
    26701 gatgtattaa gcggtactgt agacttaggt atggtatcac gtgatgtaaa tgatgaagaa
    26761 aaatctaagg gcgcatttga tttttcggta tgtaaagatg cagtacttcc taccataaat
    26821 gcggaaaatc cctatcgcga gctactttat caaaagggga tatctaatca gcaatttata
    26881 gatgtattta taacaggtaa aatcacaaca tggggccaat tgcttggcaa tggagctaaa
    26941 gagaaaattg acctgtatac cagaagcgat gctgccggcg cggctgaatc atgggcaaaa
    27001 tttcttggcg gtaaaaagca agaagattta aaaggagttg gtgtgatggg agatccgggt
    27061 ctggctcagg cagtggttca aagtaagttt tcgcttggat tcaacaatgt aagtttcgca
    27121 tatgataatg tgacaaaaat aaataacccc ggtattgctg ttgctccaat agatgttaat
    27181 agcaatggta cacttgatgc agatgaaaat ttttatggca atattgatca ggtaaatgag
    27241 gctatattaa atggaaaata cccttcacca cctgccagac ctttattatt tgtttcaaaa
    27301 ggcaatccta ctgatcctgt tgtaatcgca tttctgcaat atgtattaac agaaggccaa
    27361 aaaggtgttt ctgatgcagg gtttgttgct ctacctgctg atgttatcaa acagcaaata
    27421 gagaaacttc cttcagctaa acaataatta atcactcatc taaatgacat tgccatgcct
    27481 aaagaaatta atgatgacga tattcaaaat ataactgaaa atatcgatga gttagaaatt
    27541 actgctgtct ccacacagca aagcacaccc ttagtacaaa caggttcgaa cccgttaagg
    27601 gttattttag atgggtttat ggggaaggtg gttaaggcaa tgaccatctt ctccctttta
    27661 ttattggtat tgataattat agggttagta ttgaagtcga tacctgtttt gaaagataac
    27721 ccgttaagta cactattgtt ctcttcccaa tggagcccct ttaaaggcga atttggtttt
    27781 ttgccattta ttttatctac ggtttacgta accttaatag cggcagttat cgctattcca
    27841 cttagtttat tcactgcaat ttatttgagc gaatacgcca acaagcgatt cttaaaatat
    27901 gcggcgccaa tgcttgatat cctgggtggt attccatctg ttatttacgg catttggggg
    27961 gtgcttttga ttgttccttt tgttagggac gattttagcg cttggctggg agtcgattct
    28021 gtaagtggct actccatact tagcggaggc gttgtgctcg ctgttatggt gtttcccgtg
    28081 gtggtgcaaa ttgtttatga ggtattgaaa actgttcctc atgaattgcg agaagctacc
    28141 ttgtcgcttg gtgctacaaa gtgggaaaca ataaaaaaag tagttattaa acgagcatca
    28201 cccggaataa tagcggcatg tatactcgga ttttcaagag cttttggcga aactatcgct
    28261 gttttaatgg tagtaggaaa tgttgttcaa attcctaaag gcattttcga cgaaggatac
    28321 ccgatacctg cattaattgc caataattat ggcgaaatga tgagcattcc catgtatgat
    28381 tcagcgctga tgtttgcagc actcatattg ttggtaatag ttttagtgtt taatgtttat
    28441 gcgcgctggg tattgaacag aattgaaaaa agtataaaat agtaggtatg aaaaagcgta
    28501 agatcgaaga attgatattt aacatcatta tgcgggtttc cgcattcatt atattcggta
    28561 gcttgattgc gatttctgct accatcctca ttaagggtgt gcctgccatg aatttggata
    28621 tgattaccaa gacaccttca ggtgggtttt tcttaggaaa agaaggtggt atccttaatg
    28681 ctattatagg ctcattttat ttatctgttg gtgctaccat ttttgcatta atgatttgca
    28741 ttcctgttgt gatttatata aacgtctatg caagtgaaaa ttcaaaaatt gtacattata
    28801 cgcgtttgag ccttgatatt ttgtatggta ttcccagcat cgttttcggc gcattcggat
    28861 ttgtgttgat gttgttcctt ggtatgcatg ttagcctttt ggctgggatg attactgtcg
    28921 gacttctcat catcccaatc atcgcaagaa cgatggatga agtgatgagg caagttccgg
    28981 tagagttaaa ggaagcgtca tacagtctcg gcgcaacaaa atgggaaaca gcaagtaagg
    29041 tattgttaaa gcaggcattg cccggactgg caacagcatc attacttgga ctcggaagag
    29101 caataggtga tgcggcatcg gtgatgttta caacagggtt tactgataac ataccacatt
    29161 ctcttcaaga cccggcagca accttaccgc ttgcaatatt ttttcaatta agcagcccaa
    29221 ttgcggaagt aagagataga gcctatgctg ctgcacttat tttgacagtt atcatttttg
    29281 ccataagcct gatcagtaga atattgatta agcgattttc taaaaacacc ctctaaaatt
    29341 tatttatgac ctctagtaca ataaaatcgc atataagtat caaagactta aatatgcact
    29401 atggtaagaa ccatgcgttg aaaaatattt caatcaatat acctgataaa aagctcacag
    29461 ccatcattgg cccaagtgga tgtggtaaaa caacgcttct gaaaacattt aatcgactga
    29521 ttgaaaatgt tgatggcaca aacatcaccg gagaggtatt ggttgatgga gaaaatatct
    29581 atgggaagaa tgccgatgtg acgaatatca gaaagaaaat gggtttactc aatcagcgtc
    29641 ctacacctct tccaatgagt atttatgaca atatcgcctt tggtatgagc attcacggta
    29701 ttaagcaaaa aggtgtactc aacgaaaaag tggagcattt tctacgtgta gcaagcttat
    29761 gggatgaagt aaaggataga ctgcactcgc ccgcagcacg cttatcaatt ggacagcagc
    29821 aacgcttgtg ccttgcgcgt ggactagcag ttgaacccga aataattctc ggagatgagc
    29881 ctacaagcgc acttgatcct atctctacca aacatattga acagaagttc gtcgaactga
    29941 aagaacaata tacaattgtt atggttaccc atattcttcg ccaagcccga cgtattgcag
    30001 attatgtcat cttcatgtac ttaggcgaga taatagaaca cgggccaact gaaatggtac
    30061 ttggcaatcc tcaacaccag ctaaccaaag attatgttgg tggcgccata agctgatcag
    30121 ttccgacagt gtaaatttgg ttttgaatgt tggctccgtt tgcgtgaacg gggccttttt
    30181 tcacttaaac aaggtgttta tactttaaaa acataaaaat gtgttctaat aatctactaa
    30241 atacatatac tttatagaaa aagcatattt ttgcactacc atctaaaatt gccacattat
    30301 ggaaattgcc atcgtcctca ccttattggt gctggctatt attgccttct caaccgaaaa
    30361 gatatcggtg gatattgtca ccatgacctg cgttctgctg ctcgttttaa gtggtattat
    30421 aacttataaa gaagctttcg cccctttcgg aagcgacttt atcatcatgc tggcatccat
    30481 ttttattgtg accagtgcta ttgattcgtc aggcgttttg gaattatgga catcaaaact
    30541 catgcgccga tcagccggta acaagatgaa actcattatg ccaatgattt caataacggg
    30601 attattttct tccctaatga ataacaccac cgttactgct ctcatgatca atccggcaat
    30661 ggcaattgcg aagaagtcgg gaattaattt ttcaaaaatt ctgctgcctc tggcttttgc
    30721 ctctattgtt ggcggaacct gtacgttaat aggaacctcc actaacgttg ccgtaaatgc
    30781 ctatcttgga aaaaataatt ttccggtctt aggtatgtgg gattttacct ggataggcgt
    30841 tattatgttg ctggttacgc ttctttatat ggtattgctc ggagtaagga tgttgcctga
    30901 caggaaaaat gatgaattga ctgataatta tggcatcaga tcatatttga gtgagataaa
    30961 aattttacct aactcatcac tcatcggcca ggttatacag gattcagata tcagcagaat
    31021 ggggtttaca gtgctggctg tgattcgtgg cgacaggcaa tttgcgcccg gcgaaaatgt
    31081 atcctttcgc gaaaatgata ttgtgctggt taaaggtttg atgcagcaat taattggggt
    31141 aaaaaatacc aaaggaattg atatcgttgc ggatactttg gatttctata atgaaaaaga
    31201 caacaaactg cgtctggctg aaatggtcgt tcccgggcga tctaaccttg ccggttttac
    31261 gatcgccgaa agcgatttca gacgaaaata tggttttgtg gttgtggcaa ttaatcgcga
    31321 aggaaaaaat attaccgaac aattaaataa tatacgattt gaggttggcg acatgctgct
    31381 tgttcaggga agaaatgaag cgtttgataa attgagcagt caccacgatc tcgttttact
    31441 tggcgaccac acaccacctg tcaagaattt atggaagggt tattttgcat tggcattttt
    31501 tatgctagct attgttgtta gctcaattcc ggcatttaat gtgcatgtgt ctgttgcatt
    31561 cttggcgagt gcagtattaa gcatgctggt tggcgctgtg aagcctgaag atgcctataa
    31621 gaatattgaa tggaaactat tagtgctgat tgccggtatg acagccttcg gtacggcaat
    31681 ggcaaactcc ggtgccgata aatatcttgc aggattaatt gttgattggt ttggcggaat
    31741 ggggcctatg ggtattatgg ccggatttat ggtgcttact gtgctcttaa ctcaacccat
    31801 gtcgaatgca gccgcagctc tggtcgtatt gccggtagcc cttgaagcag cgcatacgct
    31861 tgatgttaat ccgttgacat atgctgttgc agtaatgtta tccgcctccg tatcactcat
    31921 aacgccattt gaaccatctt gtatactggt ctacggtccc ggaaaatata aattcggcga
    31981 cttttttaaa gtaggtggaa tattgacttt gattttaatg gtgattattt acctcaccgt
    32041 tccaatcacc tggccgctgt aagattgact gttttgagaa ttttataaat ctctatccag
    32101 agtacaccgg caattgcgaa acatagacaa gtgattaatt gggagaatgt cagcatttcg
    32161 aaatgaaata aattgcgcac aggctctatt gtaagcgaca caactaatat tcccattgca
    32221 atggataata ttattggaat taatttgttt ttatatttaa gtgtatgcag gatggagtgg
    32281 gtgaacgatc tgttggtcaa tgttaacagc acattgctga ataggagggt actgaaaatt
    32341 gcagttcgca cctgtggatc cgacatttga tgttgcatga agtaataacc tagtcctagg
    32401 caggcaatag tgatcgccaa cccttgtata atactcaaat ttaattcttg ccacgagaaa
    32461 aaattcatcg acatttttct gggtttcctc tgcatggaac ctttttctat gggttcgttt
    32521 tcgaagacta tagaacaagt aggccccata atcagttcga gaaaaatgac gtgtatcggt
    32581 tggaagatat ccgtaaatgc ccaaccaagc aataaaggaa gtgtaacaat taaaattatc
    32641 ggcacatgga tactgataat atatcggata gccttcttta aattctcata tatccttcga
    32701 cccaacgcaa cagcatctgc cattttcatt aagtcgtcat ctaccaaaat aagtgctgcc
    32761 gcttttttcg cggtttctgt tccgcgctta cccatggcaa caccgatatg tgcagctttt
    32821 aatgcaggtg catcatttac tccgtctccc gtcattgcca ctatttcccc tttggcttta
    32881 agtgcttcca caatgcgcaa ttttgcatcc ggaaacatgc gtgcatagat atgcgtactt
    32941 tcaacagcat ccgaaagggc ggcgtctgac atttgcatca cctcattgcc gctcaatatc
    33001 tttccattgt gttgcatacc aatgatatca gcaatggaaa tggcagtagc ggcatgatcg
    33061 ccggtaatca ttttaaattg tatgcctgcc tgataaaatg tttgaatgag gtctttaatt
    33121 tttgcctttg gagggtcgta aaatgctatc aaccctaata aatcaaaact gaataaattt
    33181 tgtctggcag gcaatccatt ttcgggaaat gctgccttcg caacagccaa tacacgaaaa
    33241 ccttttccgg tgaaaatttc agcttgattt gtaacgcgtt gtattgtttc tttatctgca
    33301 ttacacaagg gcagcagtgt ttctaagcca cctttgcaac ctattttata ttcgttgtca
    33361 ttaaaaaaca catgcgtcat aatgggtgga gtgccatcta atggatattc atgcaccatc
    33421 gaaaattgca atcgcaagtc gcttaatgcc acttctgtat acagttgatg gatttgcttt
    33481 tccatctgat cgaacggctc cggctctgaa gcccacatag caaattctat taacggatga
    33541 tatgcaggtc tgcttatatt aaaatcatga acattatcat caattgcatc gtaaatggca
    33601 attggcttca tcaggttttc tgtaatagta cctgttttat cagaacaaat tacggtggca
    33661 gcacctaagg tctcgacagt atagggactc ttggcgatga catttttctt gtacaaatga
    33721 aatgcaccca atgccataaa tgtgctgaag gcaacaggga tttcttcagg caaaatagac
    33781 atagcaagag taagtccgtg taataaactg tgaatgatat ttttactggt caggtaattc
    33841 actatacata caatcagaaa tgcaattacg ccaacaatca ccatcgattg tataaatttt
    33901 ctgatttgaa gttgtaaagg tgttttttga acagcgatat cggataattt cgcaccaatc
    33961 tcacccaaac gtgtgtttaa tccaattgcg gtaaccgttg cagttgctga gccgcctact
    34021 atggttgttc cttgaaaaag taaattatca gcgctgtcgg ccgatttata taaaggaagc
    34081 gactctcctg tcaacacact ttcattaaca gttaaatctc ttgaaaatag aattgtagca
    34141 tccgccggaa taatattacc atcctcgagt aagatgatat cacctaccac tatttcatct
    34201 gtatgtatgg aaatttgttc tttgtttctg atgacttgtg ttcgcggact agtgagatcc
    34261 ttcagagcgt ttacagcatt tctgctccgt tgttcttgaa atatggaaat gccggaaaca
    34321 attcctaagg ccaccattaa tatgagacct tcttgtattt cgccaagtat gaaatacaac
    34381 accatggtta ccgacaataa aataaaaagc ggctcggcaa ctatttcgag aataatgcca
    34441 acgagcaaat ttcgctgctg tacttcctgc acattggaac catgtaattt ccgggaagca
    34501 agcacctctt catatgataa gccgtcagga gtttgataac tcataaatca aaggtaatat
    34561 aaggtgccat aacgcacatg gacattcctc gtattacata tcacaaaaat tccttatttt
    34621 ccctttccct ggaaaatgat gagcagcgta tagaataata ttttaaagtc aattgccagg
    34681 ctcatgtttt ctatgtacag caggtcgtat ttcattcgct gtaccatttc atccacgtta
    34741 ctggcgtatc cgaatttcac catgccccag cttgttagtc ccggctttac tttggtaaga
    34801 tgtttatacg agggagcaat ctttacaatc tgatcgatat aatgtcggcg ttcaggacgg
    34861 ggtcccacaa ggctcatttc acctttcagg atattaaata tttgtggcag ctcatcaaat
    34921 ctccatttgc gcattatacg gccccagggg gtgatacgtt cgtcattctc gctggataat
    34981 gccggtccgt tttgctctgc atcgatatac atagagcgaa acttataaat ggtaaaaggc
    35041 ttattatgca aaccaatacg ttcctgatga tagaaaatcg gaccttttga cgacatcttc
    35101 acacgaatgg caataaataa atacaaaggc cataatacaa tgaatcctat tgatgcaaca
    35161 gttacatcca acgctctttt gatcatcctt tgccaaccta ccatcaggtc gggataaatt
    35221 tcgattaata ctgcgcccaa tacattactc atcttcacac tgcccgacag gatatcatac
    35281 atgtcaggaa caatttttat cacgatattg ccttttcctg ctacggtatc aataagatta
    35341 ttgagcagat gatgttccga tgtctctatc gcaataatta cttcatctac attgcgctgg
    35401 tctaaaatgg attccagctg gctcaaattt cccaggcaag gcatatgtcc ttccaaatca
    35461 ctatgatgat caccattagc ttccaggtat ccgacaaatg catatcccag tgatttctta
    35521 taatgtgtga tctctctgta aattgaataa gcacgctggt ctccaccaat aataatagtg
    35581 ttgtaagcca ctacaccttt ttgcaattgc cgctttgccc tggtcagaat gatcatgcgg
    35641 acaattgttg taagcaaaaa ctgaccgaat aatagaacaa agaaaatttg ataataatcg
    35701 cgatagtcgc tgatataatc atcgagcagc aaggcaaaga atataatcaa cacgccaata
    35761 aatgttacaa caaatgtgcg tgtgatctcg gcgagtcgcg attttctgta aatgtcggta
    35821 taggtgcctg caatgaaata caagccaagc cagataaccg gtatgctaat tatcccggca
    35881 aagaaattat tgtcgctcaa cacctgtccg tcgatgtcaa atggcagatc ttcaataaca
    35941 aatttgcgat aaaagaataa accggcccat accaccattg ccgacatata gtcgaaaatg
    36001 atataggtaa gtatcccgat cttctttttg aaacgcatta ttttgtttga atgattttca
    36061 cattatcgaa cataatggaa ctcgtgtcat tatcataatt cacagctctg aattctaact
    36121 gaatactgat gggagaatat tgtgcaaaga actgaaggga aggattgagg tttacataga
    36181 tcttattcca gtaatctttt tcagtgatgg tcaaaacctc atcatacaca ggctgatcag
    36241 ttgtattaca tttcaaccat acattaaaat ccattcctgc taaataatcc aattcaagaa
    36301 acatttgctt atcggcaccg ggaaatgcaa tcggttcgct tgatgctaca cggaaagtat
    36361 ccagatccgg gtaaaagttc gcaattgctg acctgttacc ttcaaacacc aaagatggat
    36421 cagtggtagt attcagtcca acctcagttt cgacacctcc aaaagcgttg ctgctttcaa
    36481 aacgttcaag catatcgaat tgaattccgg cagatggctt gtatgtgcaa gacggatgca
    36541 aggtgtcggt tgctccggca gtaagcgttc ttgtaatggt atatgcgtta taaaatggat
    36601 atatcatcga aaccccgctg ataccatttt ctttaatacc cggatatata ataatgtcag
    36661 tttcaccttc cgttagcacg ggaacggttg ccggaagttc ataagcacca ataaaagttc
    36721 cattgatgta tatccagccg tcagttattt tactgctggc atatccctgc gtttcgagat
    36781 tagtagttaa tgtgaaatca tctatataaa catatgatgg tgtaatttct tcaggattga
    36841 tgatttcaca tcccgcaaat aatccaacag caaaaagaat gattaatatt tcccgtgttt
    36901 tatttaacat tgatatctcc ttatttagtg gaacgcagca tttttttcaa ctttttgtaa
    36961 aatcatgtgc gctacctgta atgcgcgaaa accatcttct atcgtaacag gtgtttcagt
    37021 atcatggatg atggcattgg caaacatttc caattccagt tttattgcat tgacaggttg
    37081 cacttcaggt ctgtccagtc ttacagcttt tttgctgaac tgatctcccg tattaatatc
    37141 aatggttaac gcatcaggat gatctttcgc atctgaaagc gttaatattt ctgtcacatt
    37201 atcggcaaaa tctatcgaca catacgaatc gcgttgaaat aagcgcattt ttcgcatttt
    37261 tttcatcgag atcctgctgg cggtaagatt tgctacgcaa ccattatcaa attcaagacg
    37321 tgcatttgca atgtcgggtt ggtttgatat caccttaaca ccacttgcag ataccttttt
    37381 aacattagac tttaccagat gcaatacaat gtcaatatcg tgaatcatca ggtccagcac
    37441 aacagataca tcagttccac gggggttgaa tgtactgagt cgatgcgact cgatgaacat
    37501 aggagcaaaa tgttgatctt tcaccgataa aaaagccgga ttaaatcgct caacatgacc
    37561 aacttgtgcc tttatatgtg cctcatgcga tagcgcaacc aagctcttgg cctcgcttac
    37621 tgtattggca agcggtttct ctatgaagat gtgtttggcc tttttaatgg cggccctggc
    37681 acactcgtaa tgtgaaatgg tgggtgttac aatatcaatg gcatcgcagg catcaatgag
    37741 tcgatgcata tttgtaaacc gtggcacacc atattgattt acaacctgct cagcattggc
    37801 atccgaaggg tcgtaaaaac cgatcagttc cacagttgga atttctttta gcagtttgat
    37861 gtggatcttc cccagatgtc ctgcacctaa taccccgatc ttaaccatat cttagcttgc
    37921 aaagttatgc ctttcgcaca gagtgctcaa ttttcagcaa atgaaatgtc cacctatcac
    37981 caacacaatt gaccacatga ttatacccgt cgaaatcgtt tacctttgat ccacaggtat
    38041 gcaaggatca ataaatcgga cattggggct gtttttggga cttcctctcc tttcaggagt
    38101 ccttttttgg tggtcctggc cgccaatgca tacggcaata cctatatttt tcgcctttgt
    38161 gcccctcctt tttgccgaaa ggctcatttc agatagctac acgaagtata caggcctgaa
    38221 ggtttggttc gcactctttt taggcttgtt agtatggaat cttgctacca cttggtgggt
    38281 gcaaaaggcc tctgtagccg gtggattgtt tgccaatatt gccaatccca ttctgatgac
    38341 cattccgctg atgctgatgc gaaaagtgag attgaaattc ggcaaactgg cgggatatct
    38401 ggcttttgtc gtgttttgga tgagcttcga aaagatacat ctcacctggg aacttacatg
    38461 gccttggctg acggtgggaa atgtattcgc agcgcaatat tcatgggtgc aatggtatga
    38521 aattaccggt gttttcggcg gaacactatg ggtgctgtta atcaatattt gggtatttca
    38581 tgcgataact gaatacaagc aaccaattat ctccgcaacg aagaagaaac aactgatttt
    38641 tcacgcttta ggcatactca tcattattgc cttgcctgtt ctgataagtt tgcagatgta
    38701 tcgcaactat caggataaag gtaagcctgt tcaatttacg gtattgcaac ccaattacga
    38761 tccatacact gagaaattta ccatcccata tcgtgtgcaa atggaagaaa tgttgcgcac
    38821 ttccttgcaa catgtcaaca gtgaaaccga ctttttaatt tggcctgaaa cagctattca
    38881 aaccgatgtt tggttagata aattgtcgtg gcaaaaaccg gttagagatg tgagcaaagc
    38941 aattgatagt tttccgaatc ttgtttgcct gataggaata aatggatacg aaaaatatcc
    39001 atctgccgat aaagcaacag ccactgcgcg acatctggaa tatgccagtc ctgttacagg
    39061 aaaaccggat acgatttatt ttgatgtgta taatacagca cttgctttaa atagtgaagg
    39121 gccgattggg tattatcata agtcgaaatt agtacccggt gttgaacgta tgccatatcc
    39181 ggaatacatg aaattgctga attattttac tattaacctc ggaggaattg aaggttcact
    39241 tggaattcaa aaggacagaa cggtcttttt taataaagat tctgtcggca tagcaccggt
    39301 gatttgttat gagagcattt ttggtgagta tgttggcgat tatattaata aaggggccaa
    39361 tattatttgt atcatcacga atgacggatg gtggggcgat acagatggat acaaacaaca
    39421 ttgtatgtat gccagtctca gagcaataga atcgcgcaga gccattgccc gatctgcgaa
    39481 cacgggcatc agttgtttta ttgatcagcg tggcgatatt tcgcaggctc aaccctggaa
    39541 tgttcaaagc gcaatatcag cctctctgcc cgcaaatagt gcatttacat tttatacgcg
    39601 tcatggcgat taccttgccc gatttgcttt atggttgagc ggaggtatgt tggtaatatt
    39661 attaattcag agattgttga ataaattttc gaaacgtgtc tgattctgca gaaaatgtca
    39721 gtcttccatt agaagatata tcccacaaag tgatggctct tgtgaaaccc atcggtgctt
    39781 atatgcgtga gcaacgaaaa tcattcgatg gcgttgctat ttcaaaaaaa ggtattcgcg
    39841 attttgttac ggaagtagat atgcaatccg aacgtcaact tgttgcagga ttgcgcaata
    39901 ttttccccga tgcaggattc gttactgaag agaatactgt cgaacaaatt gatacatatt
    39961 ataagtggat aatcgatcca ttggatggca cgatgaatta tgtgcatggc attccaattt
    40021 atagtatcag cattgcattg caagcaggaa ataaattcgt acttggagtt gtatatgaag
    40081 tagcgaatga tgaaatgttt tatacctggc aaggagcttc atcctactgc aatggtaaaa
    40141 ccatttcggt ttctgcatgt catactttgc aagatgcctt aattgctacc ggatttcctt
    40201 atatcagaaa tgaccagcga acgcgagaaa tttcgaatac tctaaaattt tttttagaca
    40261 atggtcggga tatacgcaga ctgggaacag ctgctacaga tttatgttat gtggcttgtg
    40321 gaaggcagga tgtatattat gaagggttcc tgaatatatg ggatatcgcc gcaggcattc
    40381 tgattttaca aaatgccggc ggcgtagctg ttaattttaa aggggaagaa aattacaatg
    40441 agggtcgtat agtaggaaca aatcctaacc tgataggtga tgtgttgcgc ggtattagtt
    40501 tttagaccat ttgcgatact atccaataaa ttattccgct cataataatg gtagcaggaa
    40561 tagtcagtac ccatgcccaa ataatttttg cggcaacgcc ccatcttac
//
LOCUS       DAOQHY010000006        39107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_399_length_39107_cov_6.549575, whole genome shotgun sequence.
ACCESSION   DAOQHY010000006 DAOQHY010000000
VERSION     DAOQHY010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 39107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39107
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_399_length_39107_cov_6.549575"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PLL28_01100"
     CDS             <1..589
                     /locus_tag="PLL28_01100"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF67936.1"
                     /translation="MGAYVTGVDLSDVAIAKAKELAATTGASANFVCCNIYDLAQHIS
                     TPFDIVFTSYGTIGWLPDLKAWAALIRQFLKADGKLIFVEFHPAVWMFDDDFKEIKYN
                     YFNTGIIEETEEGTYADRDADIHNKTINWNHGISEVVQSLLDNDLSITGIHEYDHSPW
                     PCFKHMESDGKESFYIKHIGKKIPMVYAITAERKV"
     gene            complement(592..1767)
                     /locus_tag="PLL28_01105"
     CDS             complement(592..1767)
                     /locus_tag="PLL28_01105"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNF67937.1"
                     /translation="MDKPSLHTIKGGLITTEQLLAYHKGELSEAECRRIEQLMADDPF
                     LADAIEGLQYATPEQVQKALDAIYAKVDARIAAGAKTGFGSQLRKYAAAATILVFLGA
                     TLIIMRELNKKTSESEIAFVESAQQHPAFNNADTIGMGAGDSNVEASTIEINRKSNVQ
                     PVVIENEFTASEATEDLSGDDKFSIDESINVSEGFSTVGNLDVVKPAVADAYFRTNVA
                     VLSAIPEMSNGDFLLETKSIQEVAVLSDKKQVFKRKDEDKPNKENELTKDYSGAPAGA
                     SDTETMDDFQQGDSVMMADVQPQFVGGEKALVDYFNANLELPLSVSEGVVYVRFVVQK
                     NGTIANAYVVQGINLLPNENVLKAVNNMPAWIPGKVQNVPVDTWISLPITIGKKTDY"
     gene            complement(1760..2368)
                     /locus_tag="PLL28_01110"
     CDS             complement(1760..2368)
                     /locus_tag="PLL28_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNF67938.1"
                     /translation="MFQLLFPRRRRPSEMTDEALIEAYKQSGDTSLVGELYKRYAHLV
                     YGVGLKYMKDPEEAQDVAMMVFEKLMVDLKQHQVQQFKPWLYMVAKNQCLMLIRKRKG
                     HETVELPEYNLENDEDGGMESGLTAHLDDDSDIKEAQLNRLEEGIKTLNEEQKICVEL
                     FYLQDKSYVEVAEITGYDLKQVKSYIQNGKRNLKIFLTKQNG"
     gene            2624..4192
                     /locus_tag="PLL28_01115"
     CDS             2624..4192
                     /locus_tag="PLL28_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67939.1"
                     /translation="MKNTTQNRASRLIQYSAATIGAILTQHADGQVAYTDLDPDVDLH
                     YITGDSYLLDINNDGIDDFEISAIVSSTVFTGSSSSSFTSYTYKVLVTPLLDNEVFLT
                     DDANFIEAGETIDADLTAGEFIPSAGDFNAVFFEVPSYIGLKLNTAGAINYGWLRLTN
                     DITSFTLLDYAVELTEDTGIYAQVPGGPHAVSNISTADVSDTHTAADVQVSFTIPEDE
                     SGTYSYRIFAGEISFSKMQAINADPEQYTEVFPTGSDQALTLNPGQLDYFHNPVEEFS
                     RIVVGVLSVSADNINESVYQSYIIPVVPGHDVEPVSNVEVTHVVSPYPGTSIEVNFTT
                     PDDLHGIINFLSYIVPADMLPIIDSSLIYINPNHAVLNAWEVGGGEFATDHSYEMPFT
                     GYDINGDVLTFGGQYGVCIQSHTQWPEYSGFAFESLTCSDTFTITDETIGISTMLSGE
                     LSANVVPSGIYLQSPQMMEAVELLGINGSLIYSSSIHADNTIIPLPEASGIYFVKARF
                     KDGFGIKKLNVVIK"
     gene            complement(4208..4627)
                     /locus_tag="PLL28_01120"
     CDS             complement(4208..4627)
                     /locus_tag="PLL28_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67940.1"
                     /translation="MKKYLIILISGMLFAACSEKTELTEENIAGVWNAKEFESTIPNI
                     PAEYQEAGKQEFLSSVYTLNKDNSFELRSSFFVNGAFGRWEFDPETRELSMFYEMDTT
                     HGVEKFNITALTATSMTLRQDIPSMSAFVQLELERSK"
     gene            complement(4624..5697)
                     /locus_tag="PLL28_01125"
     CDS             complement(4624..5697)
                     /locus_tag="PLL28_01125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF67941.1"
                     /translation="MKSVLSFVIAIGVSAYMQAQSPITIVSSDLPDEGESFLVTNASP
                     LIAFDGTDTGPDHNWNFSTLVPVTQDTTNWIDENDTNPIYFFLWFTSDIAEQSVSNIV
                     NDFITITDVYNFYQLDNSTFAFAGFAGTIAGIPFPIGYDDNEVILNFPADYGQISNSN
                     TGFNISIPDIGGWNESRDRSNEIDGWGQVTTPAGTYDALRLHSVINIIDTFTYDTFVL
                     PVAYTTNEYRWLSKETGIPVLQINTQTVLGVETISSIVYFEDYYESAISEEQDLNNNL
                     QIAQPVVNEAMINFTATAAGNYQFRIFNLNGEIISTSDVNISIGENRIVINVDALPMG
                     TYLLQCNNEQGEMATKQFIKIAQ"
     gene            5745..6428
                     /locus_tag="PLL28_01130"
     CDS             5745..6428
                     /locus_tag="PLL28_01130"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNF67942.1"
                     /translation="MKWVTPIICFFVIGCSAPSVKQPQFKGGQMVEVVTLYETDTTGT
                     MFLWERSDEKRPWRCVDTINVSVGRNGLAPADTTYLDHKREGDGCSPEGIFPLTRVFS
                     YNVLNNLSMPFEQVDTNDLCVDDGASVYYNMLIDRDTAQINDYNSFETMHRKDRQYAF
                     GVWVDYNTPAIAGNGSCIFLHIWSSPGHPTSGCTAMAESDMLKLIHWLDAKQFPILYQ
                     HHLKPYENN"
     gene            6474..9635
                     /locus_tag="PLL28_01135"
     CDS             6474..9635
                     /locus_tag="PLL28_01135"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67943.1"
                     /translation="MKRFLFLAISSFFLLPINAQKNNKTTAAPATEVISKNYLSGMHW
                     RNIGPFRSGRCIAVTGVYNNDQIYYAGAVGGGLWKTSDGGHAWHCVSDSIFGSASVGA
                     VEVAQSNTNVVYVGMGEVEMRGNISFGDGMYKSIDAGKTWKKIGLEQSYAIGSIAIHP
                     QNENIVYVAAQGHIWGPNPDRGLYRSVDGGATWERILKGPNDNTGCVDVKLDPSNPNI
                     IYAGMWNAYRTPYSLSSGGPGSGLFKSIDGGKTWKEITKNPGMPKGMMGKCIVTISHI
                     DHNKLWASIENKDNAGIYASNDGGDTWFLLTKENDLIQRPWYFSNIYVDPQNDNELHI
                     LSVEYWRSINGGSEFSRAETATWDNHVMWINPTNANNFIIGGDGGPTITFDDGNNWSD
                     IDIPTGQFYHVNLDNDWPYGVYGAQQDNSSIKIKSRTSGYSIGASDWHPVAGGEAGYI
                     VPDPKDSRITYGGEYDGIFSSYNEANEVYRIISVDPEQHYGAGAITAQRRFNWTFPIA
                     FSPHNNSCLYATSNFVHRSYDAGVTWETISPDLTRNDPKTLQTSGGDITLDNTGVEFY
                     GTIFTFAESPVKAGVLWTGSDDGLVHVSTDNGTTWTNVTPSNLPEWSMISYVEPSYFD
                     AGTCYLSATRYKLDDMQPYIYKTKDYGKTWEKIVNGLPANMYNRCVRNDPNVQGILYA
                     GLETGVWYSLNDGAFWQPLQLNLPLAPVTDIRIEPKERDIVIATHGRSFWILDDITPL
                     YDLAKNGDAIQKSNAYLYPVNTGYRTPGPSYYAEEASDQTGENEAVGVKIRYYLKQKS
                     EKELRLAFYESDGDLIITYSNLYDKKRTPITIKDEFYPNPKVTESGKLNAFPGSHQFV
                     WDMRYPDATGDTSATFEASLMGPLAVPGNYMVRMYIGDSLVGQQTFVIDRDPRNPATV
                     ADLQAQFDMNQRICNKLNQIADATGSIRETRDMINEHIGEDASEEIKSKGKQLIDALT
                     VIEGELYNPKILAYEDNLKFPIMLEEKLAGLNYFLQMADTAPTKSMYAKYEDLNARID
                     VQLNALNKLMSTEVKQFTQMVGE"
     gene            9873..10193
                     /locus_tag="PLL28_01140"
     CDS             9873..10193
                     /locus_tag="PLL28_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67944.1"
                     /translation="MLHPEILRQILPGLVTSVAIRFPVAYIRSITFHVHEKMVAACFW
                     QQEEQRAAPFTIGENNMRSGCYIYNYDENIRESEVEITGMDESGKAFLISMPYNRIVP
                     MIAA"
     gene            complement(10194..11582)
                     /locus_tag="PLL28_01145"
     CDS             complement(10194..11582)
                     /locus_tag="PLL28_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HNF67945.1"
                     /translation="MSGNLNTFLQKAEQKAFDAEHKRKLLFNIGKYNATVVQGKKQYA
                     DLEHVRKKVKNLKWKVMENLDKYLIEFESNFTANGGKVIWANDEKEAQQEILKILKKK
                     EARMAVKAKSMATEEIHLNSFLAEHGIESVETDLGEYIQQLDGEAPYHIVTPAMHKSK
                     DDVAKLFNEKLGTSLDLTAQELTLVARRNLREKYVKADVGFSGANFILPDIGGIAVTE
                     NEGNGRLSTSFPKTHIVVVGIEKMLTGVNDLALLWPLLATYGTGQKVTVYNTIFTGPK
                     KEVEADGPEEMYVILLDNGRSKLLAEEKQREALYCIRCGSCLNACPVYKNIGGHTYGA
                     TYSGPIGSIITPHFKGMEEFRHLSNASSLCGNCTEVCPVHIDLHTLLLYNRNEAVEEG
                     HGTFSEKMAWKMWRRSMLNRKSMDKPSPAIKNMGVNMLFKNSWGKRRAMFKFAPKSFN
                     KMWVEKNPPEDR"
     gene            11739..13019
                     /locus_tag="PLL28_01150"
     CDS             11739..13019
                     /locus_tag="PLL28_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67946.1"
                     /translation="MKQKILLLSVLLTIVPLSTYAGTTSNGPTFAGVPYEFILFGLTL
                     LGIALFHKYTLKIALSGLAAVLVYKIFVLNFHVIEHLGHEWEIMLNLLGLLLGFAILA
                     KLFEDSHIPKRIPKFLPHDWRGPFILLCFVFVLSSFLDNIAAALIGGSIAHVVFRGKV
                     HIGYLAAIVAASNGGGSGSVLGDTTTTMMWIDGVDPRNVIHAYMAAIPAFLFFGVFGA
                     LLQDKYHPMLKGSYDPPKIDLKKLFVCICILGGAIAANFILDFPAAGVWIAIILGALI
                     TKIDWNELQKAIPGSIFLLSLVLIASMMPVDALPDATWQTTMVLGFVSSVFDNIPLTK
                     LALDQGGYDWGFLAYAVGFGGSMVWFGSSAGVAISNMYPEARSVGSWVKNGWYVIVGY
                     VIGYFILLLTLGWNPHPPHKPPVNSTTTSSSQID"
     gene            complement(13119..13823)
                     /locus_tag="PLL28_01155"
     CDS             complement(13119..13823)
                     /locus_tag="PLL28_01155"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNF67947.1"
                     /translation="MKKNTSIALLGAVLLSVSACKNAPDSDEALTGDAQEVTLTEGST
                     SVNINPATSSLEWIGTKMSTYHNGTVAIKSGTLLLKDGVITGGNFVIDMPTLKSVKDD
                     DMTNGKLTGHLQSPDFFDVAKFPEAKLEITSVKPNTTGASTGGDQPEINEYTVADPNY
                     MISANLTIKDVTKNIEFPAKVTVNNSGVSAVAKFNIDRKQWGIVYKGMPDDLIQDLIW
                     FGVSIKADAPVNTTSL"
     gene            13959..14729
                     /locus_tag="PLL28_01160"
     CDS             13959..14729
                     /locus_tag="PLL28_01160"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNF67948.1"
                     /translation="MITTLLTSLCIAMLFMTGAWLFSLYKRDMSVVDGFWGLGFLAIA
                     HHWFFRLEEAQLRQFAVVYLVTIWGLRLSAHIFIRSKGKPEDPRYTAFRNEWKENTWW
                     ISYFKVFLLQAVLCFVISLPVINVLAHHEEGFSCLNVVGLILWFIGFAFESTADYQLF
                     RFKKNPANKGQVMNKGLWYFSRHPNYFGEVCVWWGIFLVSIGSGLWYISVLGPILITF
                     LLLRVSGITMLEKRYEGNDAYSLYKKNTSPFFPWWPKK"
     gene            14822..15898
                     /locus_tag="PLL28_01165"
     CDS             14822..15898
                     /locus_tag="PLL28_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67949.1"
                     /translation="MYKREVIADYLYPDFNLDLISNYTIDSAGIQLTMRMNADLTWKH
                     ITDTLGTSRSVLHTRYAYYPEAKSFIETAFSKDTLPNMGEGVLESSLVTPQGALTLYF
                     THDKKHNNRLYIYHAEENAIYIKDVPFGYEIGQNDTSVFYRTTKFKHEKVKYAAGTIV
                     SVGINDSKIEQPLFKHQSDRFQHCTFYADPYIAYWQLYSQSFYDCHTAFHNKIYIHYN
                     NSLTDSIQYVDEYIKPYLSLKNDTLSLVIYEFDTLLLKSFLSLYQLTSGQFIRSWKSQ
                     EWQEVVNENFSFIKNYRIVGSVIFVETERRVSSEDVTKSIEVPYVLSPNGLPFTKKGT
                     IPIITAFDLHSNAFLGYPQISIIN"
     gene            16051..16446
                     /locus_tag="PLL28_01170"
     CDS             16051..16446
                     /locus_tag="PLL28_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67950.1"
                     /translation="MSTVKKTIVPVLLSTVWISVNEFIRNQLVLQNYWTDHYNNLGIT
                     FPAEPVNGAVWGIWSLLFAVAILIISKRFSLRDTTLLSWLVGFVLMWVVIGNMGVLPT
                     GILIFAIPWSLVEAFGAAYISHYFTKPRA"
     gene            16446..17150
                     /locus_tag="PLL28_01175"
     CDS             16446..17150
                     /locus_tag="PLL28_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67951.1"
                     /translation="MLSATAASAILAYGGETFWKEAKNIEAEVSVRGWAFRLKWRPFF
                     DHVHIKLDAEHPFSQLTPIGSDLGVVAVLDGDDVHLEDLTGEISKFRSGAKHYFPYGR
                     RLLYWDDLDMGYFANYAFWNYFTLPKLLLRDDIKWIETKYGVLHAIFPDHIPTHCKEQ
                     QFTFDTTTGLLKQHDYTAEVISGLATAAHVIHEHQTKNGITFASRRIVTPRTKTGKAM
                     KAPVLIDITVHDLKIS"
     gene            complement(17153..18160)
                     /locus_tag="PLL28_01180"
     CDS             complement(17153..18160)
                     /locus_tag="PLL28_01180"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNF67952.1"
                     /translation="MKIAVVGATGLVGQMMRRILEERNFPVTEMIPVATERSVGKKVK
                     FRGEDVEVVGMETAIAMRPQIALFSAGGNTSLEWAPKFEEVGCWVIDNSSAWRMDPTC
                     ALVVPEINAHDIVKYGRKIIANPNCSTIQMVMALNPLHKLYSIKRIVVSTYQSVSGSG
                     AKGVMQLYNERSSGCRVAENPAYPHQIDKNVIPQVDVFMENGYTKEEMKMHNETRKII
                     GSDNIMVSATTVRVPVSGGHSESVNVEFERDFSIDEVITTLQQTEGVVVEDDLVNNVY
                     PMPINAEGKDEVFVGRIRRDYTQANTLNFWVVSDNLRKGAATNAVQIAEYLISSGIVK
                     D"
     gene            18288..20891
                     /locus_tag="PLL28_01185"
     CDS             18288..20891
                     /locus_tag="PLL28_01185"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNF67953.1"
                     /translation="MKSIYTLCLVMLACAVGQAQSFFDDFNDADISDWGGTPEYFIAN
                     GTGELQLNGDCAAGGDHYISQALPTLDSAVWSFQVHLDFDPSNSNHTRVFLQSDIADL
                     SGDVYGYFVRVGEDGTGDVVKLWRSNGASASVVITGTSPVAFSPVVGVKVVRTADAEW
                     QLYVDADGGTDYVLEGTDIDDTYNGGNYAGIECRYTSTRCTLFYFDDFSIDPLYIDVT
                     APAIQSVTVLSSTQLLVDFTENITLVTAETESNYTVTGGVGSPIDATRDVIDNSKVTL
                     TFLSEFPEGITLTLAVNGVTDDAGNITSGATANFSVYTTQLYDIVINELMADPSPPVA
                     LPEDEFLELYNTTDVDIDITGFTLSDASGPSDPFTSYILPAHGYVILCDDGDISSFLA
                     YSNVLPVAGFPSLNNDGDDMMLSDQNGIEIHRVTYTSDWYQNAIKADGGWTLEMIDPA
                     NPCQGIENWIASDAVEGGTPGAQNSVFGSNPDLVAPALLEVFPQTLDTLTLTFSEAVI
                     GENIITADIQITDETGASIAIAAIILNPDNDAKIGVVLSSPLSGGVIYTCTVMHAADC
                     SGNDILLFNTITFGIPEPVSAGDIVINEILFNPVTNGVDYVELYNRSNKIIDLSTLII
                     AELELLDSTVIDEFAYVTSDGQLLFPGNYICVTSDIEQVLAQYFTVDTGNFVKNNNLP
                     GYNDNEGIVVLYDATLNEIDHLHYFDDWHYALLDDENGVSLERVNYAFATQDENNWHS
                     AASDVHYGTPGYQNSMFGEIHAQATIGVEYPVFTPDGDGYHDLLVITYLTESEGFTGA
                     FTLFDAQGRKIKTLTTNELLSREGFITWDGVTDDGSKANSGIYIIYAELFNTEGVVER
                     FKLKCTLLRKQ"
     gene            complement(20892..27032)
                     /locus_tag="PLL28_01190"
     CDS             complement(20892..27032)
                     /locus_tag="PLL28_01190"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNF67954.1"
                     /translation="MEGKKWIVAGAIVAVAAMTGLLAAVMTPTKTWAGYDDAWKAVDS
                     LDRISMPESALKLVNEIYDHAKAENISPQIIKSLIYRGKYTNMLEEDGEEKAIALFEK
                     EIAISDGVTKAVLQSLTANLYWQYYSNNRWQISQRTQTEELLSNDIATWDIKALLNKT
                     NELYNASLSQRELLKKTPSTLYVEILLKGNAYGQFLRPTMFDLLGNEALSFYMNSEST
                     LDKPAYAVNIANPILLESAKQFSGYSFNAADSVITQLPAVRILQELTTAHLYDPYPDA
                     LIDLNLSRYLYAKNILSIANVDSLYLQALERDEKQYARDSSSARISLAIATWHMGKRY
                     QYQPLEGDDYKWELRTAETYLNKAIAAFPKSYGGILCKLELNELVRKRLEELNIEQEQ
                     IPGAPILASIKYTNVDKIHLQLLKASANEIVSISSMDYSDRKAALKKLVVERSWTQNL
                     IVDGDLQSHRVEFKIDALGLGSYFLLASANDNWMSDSADVYIRDIWVTNMAYIAESGN
                     GDESDYKRFYVVDRTSGLPMKGVNVTTFRNDYDYSTHKYKESELQKFVTDGNGMFELR
                     DPIIRNTYSFGFTLQNGKDILKTNKLFSIAKSQTQPDREIQHTILFTDRAIYRPGQTV
                     YFKGIMYSETAFGRTKEVLKNHKTTVRFFDVNYQEVGNLELVSNDFGSFSGSFTAPAS
                     GLTGQMQISNETGAVYFSVEEYKRPTFYVELEDLKGDYILNDTISVNGTATAYSGAVI
                     DNAQIQYRVVRSVHMPYWYGDYYFRKSYPVSNADQEIVSGVSATDAEGKFVIPFVAKS
                     QVENVSGLQYTYTVYVDVTDVSGETRSSQTNVTVGKINLQLSADVKDAYHVNSDIAIK
                     ITTHNLNGQPLPATGKVTIFAIDAPKRLMRYRLWDTPDLHLMSEADFIKQFPRDQYAA
                     ELDAGSWKTGAALASDTWNTKENDSVLLPTQGLKQGVYRVVFEALDKSGETVRSEHTI
                     NLLNDGSYAAYPEYFHSVKSENSVEPGNSAIVQVGSSTSGSKALVLIKRYHQPTKAQW
                     VSMMEVAGKKSGVSMMDLEIPVSETDRGGIGVQVMMIKDGREFSKVYNITVPWTNKQL
                     QVKLETFRDKLQPGAKETWKIQVSGEKSDMVSAELLASMYDASLDAFRTNYWGDVYYP
                     ANYLYTSLSAEAMGQSYGWQHNGYFNYYATYPTIEYNQLNWFGFSLNGYQFLNYDIGA
                     AADFVDQRSRSIVTGEGLDNLMPAMMQEVQITKTNKGAVGNGFYADLEVVKEKNQQEV
                     PAPLGKIPMRNNFNETAFFFPQLETDSLGNVSFSFTMPDALTTWKFMAYAHTKDLQTG
                     SAMASVITQKELMIQANMPRFLREGDHITLAAKISNLTDKDISGSAQLELFNAVNMQP
                     VNAVFENKQATVSFTASAKGSTSVQWEVHVPEGIDAIVYRVKAGNETFSDGEENGLPV
                     LTNKILVTESIPLWVRGGDSKSFVFDKLLKSGNSSTLRNQNVTLEYTSNPAWYAVQAL
                     PYMMEYPYECAEQVFSRFYANSIAAHIVNSDPEIKRVFDSWKKNPSSLVSNLEKNEEL
                     KSLMLEETPWVLQAQNETERKKRVALLFDVNKMQSEQRAALQKLQKMQLPDGAWPWFS
                     GYKDDPYITQYIVTGLAHLERLSATEYNTAGEVDAMLRSAMPYCSANMYETYTRLEKN
                     KVEKSSYVPDNLILQYLYAASYFDEKRFPLTKNEAEAKAYFIAQIKTYWTSYSLYQKG
                     MIAIILNRNDQVKTAQQIVASLKENSIKSEEMGMYWKSNIAGYYWYQAPIETQALMIE
                     VFAEVANDKNAVDALQIWLLRQKQTTDWKTTRATAEACYALLSRGLNLLANTELANIT
                     IGNLQVKPEQTELGTGYFKSTWLGTNINPAMGNITITPPANNLLSYGAMYWQYFEEMD
                     KVTSAETNLKLKKQLFLEVNSASGPVLRAVDEKTELHIGDKVIVRIELKSDRDMEYVH
                     MKDMRASGFEPTNVISSYKWQDGLGYYEATGDASTNFFISYLRKGTYVFEYPVRVSHK
                     GSMSNGITSIQCMYAPEFSSHSEGSRVTVK"
     gene            complement(27113..27676)
                     /locus_tag="PLL28_01195"
     CDS             complement(27113..27676)
                     /locus_tag="PLL28_01195"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNF67955.1"
                     /translation="MTMHRHLVLAAVVTLPGLGGLGLGLMYFFHPDEIARLTSVSGQE
                     LGFQLLLGTGFAAVSLAALLGVLKLALLKPVVSFFAQLLTQYAISAPVILILSLCAGV
                     GEELLFRGAIQPWLGIWFTAFIFILLHGYLNPLNRPLMFYGTILLVVSAGFGYLCLHT
                     GLYAAMTAHTVIDIALMMHLRKVAKQS"
     gene            complement(27673..28680)
                     /locus_tag="PLL28_01200"
     CDS             complement(27673..28680)
                     /locus_tag="PLL28_01200"
                     /inference="COORDINATES: protein motif:HMM:NF039709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6263 family protein"
                     /protein_id="HNF67956.1"
                     /translation="MIKLRIAVVAGLMIAALFSTSCNKSSSGDKDNQSADTIVAASND
                     TAIAINLESPVADERVLLRFMPEEGKTIYIENNTTFASDESVDTMRIKANSTKYVKAK
                     LVVKGKEKEGYAIDLTLVDVHKTVKDDSGTINYQYNKPMTNPEDEMDRKVEDCMVNST
                     LTLIMTEQGENIDVRGYEAIIKKVKAIVGAQVPDQYIAANIGSPTDNLEYFFISYPDS
                     AVKIGDTWDVEVPSVMQGVPITLKTVFALADRRDGIAYINFNTVASVDKSKLPPEMAA
                     EVDKIHFNAGVKGTGEIFENTGWPRLMKFTQYLDVSDSYQGHATSSKQTGVSTIRIVQ
                     Q"
     gene            28727..31840
                     /locus_tag="PLL28_01205"
     CDS             28727..31840
                     /locus_tag="PLL28_01205"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNF67957.1"
                     /translation="MNTMESTARQDKSFAEAYAQLNDAQRRAVDHIEGPVLVVAGPGT
                     GKTQILAVRIGKILAETDAMPENILCLTYTDAGTVAMRKRLLDFIGADAHRVHIHTFH
                     AFCNDVIQAHLDYFGKRELEPITDLENVMILESILEELPTGHLLKKLKGDLSYEVARL
                     NNLFRMMKEEDWDEKRISDNIDVYLADLPNRTEYTYQRVNNKTGVKVGDPKVSKIAEE
                     QERMEKLRAAAQLFPKYQARMKEAGRYDYSDMILWVVRAFRDPSAVGENMLRNYQERY
                     LYILVDEFQDTNGAQNEILQLLISFWEVPNVFAVGDDDQSIYEFQGARVKNILDFYTA
                     YQEHVEVIVLTDNYRSHQAVLNAARVVIEQNQERLINKIEGLTKQLTASHPSRQKIDE
                     PKVIEYENPTQEHAGILREVETLLKQGVNPEEIAILYHRHKHADDLITACEKRGIPYQ
                     VRKKINILEVPIVQQMLTMLRYLNEEAQSAHSAEHLLFEIMHYPFIGIHPHDVAALSA
                     WISSRRDPEITWRTVMADPSKLGQIRLRAHESITNLEKNITHWIQETFNLTLQMLFEK
                     VLNESGLMQYILKNNDRVFLLEAISTLFKHIKAEGMNRPRITIRNYMEVLDQMETHGI
                     QLSIEKTIAQKGGVQFITTHSSKGLEFEYVFLLGCNASNWEKSRGDNRMFAMPDTLTM
                     SGEENKVESLRRLFYVAMTRAKQHLYISYAAASNEGKVLEASQFVSELLAGENISVEK
                     CNIDAETMMAYQLTSLSPTKEVHIDLFDKDFIASKVEHFVMSASALNTYLDCPLRYYF
                     ERIIGVPGAKNDSIAFGNAVHYALRKLFERMRADERQQFPPESVFISDFMHEMERNED
                     AFTEKQFNNRMELGNTLLSDYYQTHIHQFNKVVVTEYPIRNAEMEGIPITGVFDKIEF
                     IGKDVNVVDYKTGAVKYAREKLLPPNEKNPDGGDYWRQIMFYKILLDSQRTKPWKMIS
                     GEVDFIEKDESGEFVRHKFAIASDQVDIVKAQIRDTYARIKNLEFTTGCGDDDCDWCN
                     FVRNLEQ"
     gene            31931..32893
                     /locus_tag="PLL28_01210"
     CDS             31931..32893
                     /locus_tag="PLL28_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNF67958.1"
                     /translation="MQAAVYTSYGAPDVVTVRDIPTPRPGEKDVLIRVHASTVNRTDC
                     GFRSAEYFISRFWSGLFKPKRQVLGCEFAGVVDSVGSSVTMFKPGDKVFGFNDRTFGG
                     HAEYICQPENSAMDIIPEHLNFEQAAALTEGSHYALNDIYAAHVKAGDHVLVYGATGA
                     IGSAAVQILKYLNAHVTAVCGTAHVDLIKSLGADVVIDYMQQDYTQQGLTFDLVFDAV
                     GKTSFGTCKPVLNEPGIYISTELGKNGQNVWYSIFNAFTGNKKVLFPLPVYDKEMISF
                     IRKIADEKHFTPVLDKTYALKDIREAYAYVETGTKVGNVIIRCI"
     gene            32948..33565
                     /locus_tag="PLL28_01215"
     CDS             32948..33565
                     /locus_tag="PLL28_01215"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001886.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HNF67959.1"
                     /translation="MANNYSAFTAHPWHGIHIGDKAPEVVNAYIEIVPTDTVKYEIDK
                     PSGFLKIDRPQKYSNVLPCLYGFVPKTYCGPGIGNFCSQQTGRAGIVGDGDPLDICVF
                     TERNIPHGNIIVPAKLIGGFRMIDGGQADDKIIAVLPFDEIYSMWEYIQQMPSSLVER
                     LRHYFLTYKALPGAPNKAEITHLYGREEAIRILELARKDYAELIK"
     gene            33728..35533
                     /locus_tag="PLL28_01220"
     CDS             33728..35533
                     /locus_tag="PLL28_01220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF67960.1"
                     /translation="MGEKASLYAPLLVETAWEVCNQVGGIYTVVRSKAPAIVDKWSGN
                     YCMIGPLLNAKLPAEFEEVFSYDDVFVEAVTAMRNKGYEVHYGRWLTDGKPKVILMSP
                     MQVYDKMKDIRTDLLRDHKINIFNPDDLLQQVIAFGYVVAQFFSTLEATAKGRPVIAH
                     FHEWMTGMPIGHIRKEGLKVKTVFTTHATLLGRYIAGNDEQFYRHLSNYDWLQMAQKF
                     YIEPQTRLERMAAADCDVFTTVSDITGKECEFLLGRKPDMILPNGISLTRFLATHELQ
                     NLHGQFRDKIDEFVMSHFFHSYTFDLNQTIYFFTSGRYEYHNKGYDLTLEALRRLNER
                     MKKENAQKTVVMFFITKRPFETINPLVLHARAAMDDIRSTCEEMLKQVKDKLFHAAAT
                     NVDPNVPDLNEFIDDYWKFRYRKQIQGWKSDFWPIVVTHNLKDDGGDEILNYLRSNDL
                     INKPTDKVKMVYHPDFVAPTSPLFGMDYPQFVRGCHLGLFPSYYEPWGYTPLECVARG
                     VPAVTSDLSGFGNYCQSKFRNLDERGIYIVRRKTKTFDESADELCDIMFNYTQLARRD
                     RIILRNTVESYAEHFDWSNLAANYEKAYQMVLQQE"
     gene            complement(36137..36901)
                     /locus_tag="PLL28_01225"
     CDS             complement(36137..36901)
                     /locus_tag="PLL28_01225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF67961.1"
                     /translation="MKKIYAFFTALLITGALTAQVNVTYMVDITNYLAAGNVLGANGI
                     RVGGDFGATAGMNGAAAMAVWTPSDPTCALVDMGGNVWGITVTYPTASVGLTQYYKFV
                     NNDWGTNEGTDAGNTIGADGCGVDDGAGNVNRTLVIPDADIMYLFCWDACYRCDGSDP
                     VVLSVEDFNAISGLSVSPNPTVTNTNINFNLSEASDVTIGVYNLMGQEVLTVNNGTQS
                     AGNHSYSLDMSNLPAGNYVYHVVAGSSVTSGNIVKL"
     gene            complement(37021..38196)
                     /locus_tag="PLL28_01230"
     CDS             complement(37021..38196)
                     /locus_tag="PLL28_01230"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HNF67962.1"
                     /translation="MSVIDQAIVDITSVIRHDVFSAPAASRIYAYTTIAGNIGMSGNS
                     DLPTPLRSKLVLSSGVSPSDTLAAMAMYVCAQRFIYSDSMLLEKCKKRLGSEIITSQY
                     FMEAQTINASMADWINADNYTNAIASTRYTPLKGSLNWVPTPPDYAQGTDPHWGEIMP
                     FCYKYDKSILPVPAFVLDSTVIAKEAQKLYVLTMSLDSEQMEIALFWDDNPFILRQNG
                     HLQYAEKKVSPPGHWLDITRMALLATGADETAAMNAYMLVSIAMADAMIAVWDIKYET
                     QLIRPETYINTYIDSEWRPYLQTPPFPEFPSGHSAVSAAAATILTSLFGDNFAFTDAT
                     ETTFGLPARGFKSFEDAASEASQSRVYGGIHFVPAVTYGAELGKNSAFELINITNRR"
     gene            38488..>39107
                     /locus_tag="PLL28_01235"
     CDS             38488..>39107
                     /locus_tag="PLL28_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67963.1"
                     /translation="MNSHITHAQITTDITPVPGLINFQNLRDYCLNASGDEDFFTIQS
                     PNGEISQIATRKKINGNWSSPELMPFCDAFMYLEPFLTTNGLTLYFVSDRPLNDGDTI
                     KKDFDIWMVKRAAITAAWSKPVNLGTPVNSTNDEFYPSLTTSGDLYFTMAAPEGMGKD
                     DIYMCKMTSTGYEKPTILPSPINSAGYEFNAFISPDGNILIYTKYNA"
BASE COUNT        11127 a         8146 c         8133 g        11701 t
ORIGIN      
        1 catgggtgca tatgtcaccg gcgtcgacct tagcgatgta gccattgcaa aagctaaaga
       61 gttggccgca acgacaggtg cttcggctaa ttttgtttgt tgcaatatct atgacctcgc
      121 gcagcatatt tctaccccat ttgatattgt ttttaccagc tatggtacga taggttggtt
      181 gccggatctg aaagcatggg ctgcattaat taggcagttt ttgaaagcag acggtaaatt
      241 aatttttgtt gaatttcatc ctgccgtttg gatgttcgac gatgatttca aagaaatcaa
      301 atacaattat ttcaatacag gaattattga agaaacggaa gaaggcacct acgccgatag
      361 ggatgccgat atacacaata agacaataaa ctggaatcac ggcataagcg aggtggtgca
      421 aagtttatta gataacgatt tatctattac cggcattcat gagtatgatc attcgccgtg
      481 gccttgtttt aagcacatgg aaagcgatgg taaagaatca ttttatataa aacatatcgg
      541 gaagaaaatt cccatggtgt atgccatcac cgcagagcga aaagtctgaa atcagtaatc
      601 agtttttttt ccgatagtta ttggcaggct aatccatgta tcaacaggaa cattttggac
      661 ttttcccgga atccacgccg gcatattatt cacggcttta agcacgtttt cgtttggcaa
      721 taaattaatt ccctgaacaa catatgcatt tgcgatggtg ccattttttt gaacgacaaa
      781 tcgcacataa acaacacctt cactcacgct taggggcaat tcaagatttg cattgaaata
      841 atcgactagc gctttttcac cgcccacaaa ttgcggttga acatctgcca tcataacaga
      901 atcaccttgt tggaagtcgt ccattgtttc tgtatctgat gcgccggcgg gagccccgct
      961 gtaatctttt gttaattcgt tttctttatt tggcttgtct tcatctttac gtttgaagac
     1021 ttgcttttta tcacttagca cggcaacctc ttgtattgat tttgtttcaa gaagaaaatc
     1081 tccgttgctc atttccggaa tagcgcttaa caccgctaca ttggtgcgga aatatgcatc
     1141 tgcaacggct ggttttacaa catctaaatt gccaacagtg ctgaaacctt cggacacatt
     1201 tatgctttca tcaatactga atttatcatc tccggataaa tcttcagttg cttcagacgc
     1261 ggtgaattcg ttttctatta ctaccggttg aacattgctt tttctattta tttcaatggt
     1321 acttgcttct acatttgaat cgcctgcgcc catacctatg gtatctgcat tattaaatgc
     1381 gggatgttgt tgtgccgatt ctacgaaggc tatttctgat tcacttgttt ttttattgag
     1441 ttcgcgcatg atgatgaggg ttgcaccaag gaacaccaat atggttgccg cggcagcata
     1501 ctttctcaac tgactgccaa aaccggtttt tgcacctgcg gcgatgcgcg catctacttt
     1561 ggcgtaaata gcatccaaag ctttttgcac ctgttcgggc gttgcatatt gaagcccttc
     1621 gatagcatct gccaaaaatg gatcatcagc cattaattgt tcaatacgac gacattcagc
     1681 ttccgacaat tcacctttgt ggtatgccag aagttgttcg gttgtaatta aaccgccttt
     1741 gatagtatgt aggctcggtt tatccatttt gcttcgtcaa aaatatcttc aaatttcgtt
     1801 ttccgttctg aatgtaactt tttacttgtt tcaagtcgta tccggtgatc tcagcaacct
     1861 caacatagct tttatcctga aggtagaaca gttcaacaca gattttctgc tcctcattca
     1921 gggttttaat gccttcctcc agcctattaa gctgtgcttc cttgatatca ctgtcatcat
     1981 caagatgcgc ggtgaggcct gattccatac caccatcttc atcattttca agattatact
     2041 ccggcaattc tactgtttca tggccttttc gcttcctaat cagcatcagg cattggtttt
     2101 tggctaccat atacagccag ggtttgaact gctgcacctg gtgctgtttc agatcgacca
     2161 tgagcttctc aaacaccatc atagcaacgt cttgagcctc ctcggggtct ttcatatact
     2221 tcagaccgac accgtaaaca aggtgcgcat accgtttata aagctctccc accaatgagg
     2281 tgtcgcccga ctgcttgtaa gcctcaatga gagcctcatc agtcatttcg gaggggcgcc
     2341 gccgacgtgg aaaaaggagc tgaaacacag ggtaaagata atatatgagg tttaagcgac
     2401 aatgtaagcc gcggttaatg atcccggtta acggttttcc ggcaatatgg tctcattcgt
     2461 tggcgtgagc acgacaagaa agtgcctgtt ggcgaaaagg gcttcaatca attaatggat
     2521 agtttcatgc agaaaatgga gtatttcgct caaaaccgtt tgtccggttt taggttagtt
     2581 gctactttcg aactgtaatt attcatcaac ccaatcttta actatgaaaa acacaaccca
     2641 aaacagagca tcacgtctta ttcaatattc agctgctaca attggagcaa tattaacgca
     2701 acatgcagat ggacaagtag cctataccga tctggatccg gatgttgatt tacattatat
     2761 aacaggagat tcctatttat tagatattaa caacgatggt attgatgatt ttgagatcag
     2821 tgcgattgtt tcatcaaccg tatttacggg aagtagttct tcatcattta caagttatac
     2881 ttataaagtt ctggttaccc cgttattgga caatgaagtt ttcttaaccg atgacgcaaa
     2941 ttttattgag gcaggtgaaa caattgatgc cgatttaaca gcaggtgaat ttattccttc
     3001 agcaggcgac ttcaacgctg tattttttga agttccatct tatataggtc ttaaattaaa
     3061 tacagccggc gctatcaact atggttggct aagacttaca aatgatataa caagttttac
     3121 tttacttgat tacgcagtag aattaaccga agataccggt atttatgcgc aagtgcctgg
     3181 tggcccgcat gctgtatcca atatttcaac agctgatgtt tctgataccc atacagccgc
     3241 cgatgtgcag gtatcattta ccattccgga agatgaaagc ggcacatatt catatcggat
     3301 tttcgcgggt gaaattagtt tcagcaaaat gcaagcaatt aatgccgatc cggaacaata
     3361 tacagaggtt tttcctaccg gaagtgatca ggcgcttaca ttgaatccag gacaactgga
     3421 ttattttcac aacccggtag aagaattcag tagaatagta gttggtgttt taagtgtaag
     3481 tgccgataat atcaacgaaa gtgtttatca atcatatatt atacccgttg tacccgggca
     3541 tgatgtggaa cctgtcagca atgttgaagt aacacatgta gtcagcccct atccgggaac
     3601 atcaattgaa gtgaatttta ctacacctga tgatttacac ggtataatta attttctgtc
     3661 gtacatagtt ccggcagata tgttgccgat aattgattca tctttaatat atattaatcc
     3721 caatcacgct gttcttaatg catgggaggt tggcggaggc gaatttgcaa ccgatcacag
     3781 ctatgaaatg ccatttacgg gatatgatat caacggcgat gtgctgacct ttggcgggca
     3841 gtacggagta tgtatacaat cgcacactca atggccggag tatagtggct tcgcttttga
     3901 atcgctaaca tgcagcgata catttacgat aacagatgaa acaataggca tttcaaccat
     3961 gctttccggt gaactatcgg caaatgtagt gccgtccgga atctacttac aatcacctca
     4021 aatgatggaa gcggttgaac tattaggcat aaacggaagt ttaatttatt cctcatccat
     4081 tcatgcagac aataccataa ttccactccc cgaagcatca ggaatttact ttgtaaaagc
     4141 ccgctttaaa gacggctttg gtattaaaaa gctgaatgtg gtgattaaat aattggcctg
     4201 aattcaatta tttactgcgc tctaattcga gctgcacaaa ggcactcata gatggtatat
     4261 cctggcgtaa agtcattgat gtagccgtca gcgccgtgat gttgaatttt tccacgccat
     4321 gggtggtatc catttcgtag aacatactca actctctcgt ttcgggatcg aattcccacc
     4381 ttccgaaagc gccgtttaca aaaaaagaac tgcgcaattc gaatgaatta tccttgttaa
     4441 gcgtataaac acttgataaa aattcttgtt ttcccgcttc ctgatattcg gccggaatat
     4501 tgggaatggt gctttcaaat tcttttgcat tccaaacacc tgcaatattt tcttcggtta
     4561 attccgtttt ttcgctacat gccgcaaaca acatcccgct aataagtatg atcaggtatt
     4621 ttttcattgt gctattttta taaactgttt agttgccatt tcgccttgct cattattgca
     4681 ttgcaataga tatgtgccca taggcaatgc atctacattt atcacaatgc gattttcacc
     4741 aatggaaata ttcacatcgc ttgtagaaat tatctcgcca ttaaggttaa aaatacgaaa
     4801 ttgataatta cctgcggcag ttgcggtgaa atttatcatc gcttcattga caacgggttg
     4861 tgcaatttgt aaattattat ttaagtcctg ttcttcggaa atggcacttt catagtaatc
     4921 ttcaaaatat acaattgacg aaatggtttc tacgcctaaa actgtttgag tattaatttg
     4981 taaaaccggt atacctgttt ctttacttaa ccagcgatat tcattagttg tatatgcaac
     5041 aggcaacacg aatgtgtcat aagtgaatgt atcaatgata ttaattacag aatgtaaacg
     5101 caatgcatca taggttccgg caggtgtagt tacttgtccc catccatcaa tttcattgct
     5161 gcggtctctt gattcattcc atcctccaat gtcgggaatg ctgatattaa aaccggtatt
     5221 tgaattgctg atttgtccat aatctgccgg aaaatttaat atcacttcgt tgtcatcata
     5281 gccgattgga aaaggaattc ctgctattgt gccagcgaaa ccggcaaatg cgaaagtgct
     5341 gttatcgagt tgatagaaat tatatacatc tgtgatggtg atgaaatcat tgacaatatt
     5401 tgacaccgat tgttcggcga tatcgctagt aaaccacagg aagaagtaaa tgggattggt
     5461 atcattttca tctatccagt tagttgtgtc ctgggtaacg gggacaagtg tgctgaaatt
     5521 ccaattgtga tcaggacccg tatctgtgcc gtcgaatgca atcagagggc tggcatttgt
     5581 tacgaggaag gattcgcctt cgtccggaag gtcactgctg acaatagtga tgggcgattg
     5641 agcctgcata tacgcagata cgccgatcgc tatcacaaag ctgagaactg atttcatatt
     5701 acaaagataa cagaccttaa caagggaatg cgtatttttc ggggatgaag tgggtgacgc
     5761 ctataatatg cttctttgta attggttgca gcgcgccatc ggtgaaacaa ccgcagttta
     5821 aaggtggtca gatggtagag gtggtcacac tgtacgaaac agacaccaca ggaaccatgt
     5881 ttttatggga gcggagtgat gaaaaaagac cctggcgctg cgttgatacg attaatgtaa
     5941 gcgttggcag aaacggctta gcacctgcag ataccaccta cctagatcac aagcgcgaag
     6001 gagatggttg ttcgcccgaa ggtatttttc cattaacacg tgtattcagt tacaatgtgc
     6061 tgaacaattt atcaatgcca tttgaacaag tagacacaaa tgacctgtgt gttgatgatg
     6121 gagcttctgt ttattacaat atgttaattg atagggatac tgcacaaata aatgactata
     6181 acagcttcga aaccatgcac agaaaagacc ggcaatatgc ttttggtgtt tgggttgatt
     6241 ataatacccc ggctattgca ggaaatgggt catgtatttt tttacatatc tggtcgtctc
     6301 cgggacaccc tacaagcggc tgcaccgcta tggcggaatc tgatatgtta aagttgatac
     6361 actggctcga tgccaaacaa tttccaattt tatatcagca tcacctgaag ccatatgaaa
     6421 ataattaaac ttagcagtat tccatatttg tcagacgaaa tatttcaatg tatatgaaaa
     6481 ggtttttatt tctcgcaatc tcatcctttt ttttgctgcc aataaacgca cagaaaaata
     6541 acaaaaccac tgccgctccg gcaaccgagg taatatcaaa aaattattta agcggtatgc
     6601 attggcgaaa tataggtcca tttcgctcag gaaggtgtat cgcggtaacc ggtgtttata
     6661 acaacgatca aatatattat gcaggcgctg taggcggagg attatggaaa acatcagacg
     6721 gcggacatgc atggcattgc gtgagtgaca gcattttcgg atctgcaagt gttggtgcag
     6781 ttgaagttgc acaaagcaat accaatgtag tatatgttgg tatgggtgaa gtggagatgc
     6841 gcggcaatat ttcatttggc gatggcatgt ataagagtat tgatgccgga aaaacctgga
     6901 aaaaaatcgg tcttgaacag agttatgcca tcggcagtat tgccattcat ccgcaaaatg
     6961 aaaatatagt atacgtggcc gcacaaggac atatttgggg tcccaatccg gatcgcggac
     7021 tatatcgaag tgtagacgga ggtgcaactt gggagcgcat tttgaaagga ccaaatgata
     7081 atacgggttg cgtagatgtt aaattagatc caagcaatcc gaacattatt tatgccggta
     7141 tgtggaatgc atatcgcaca ccttattcat taagcagcgg aggaccggga agtggtttat
     7201 ttaaaagtat tgacggtgga aagacatgga aagaaattac aaaaaatccc ggtatgccaa
     7261 agggcatgat gggcaaatgt attgttacga tttcgcatat cgatcataat aaattatggg
     7321 cttcaataga aaataaagat aatgcaggaa tttatgccag caacgatggc ggcgatacct
     7381 ggtttttgct taccaaagaa aatgacctca ttcagcgtcc ttggtatttc agtaatatat
     7441 atgtagaccc gcaaaatgat aacgaattac atattttatc tgtggaatat tggcgaagta
     7501 ttaacggcgg aagcgaattc agtcgcgcag aaactgcaac ctgggacaat cacgtaatgt
     7561 ggatcaaccc tacaaatgca aacaatttta ttattggtgg cgatggtggt ccaaccatta
     7621 catttgatga tggcaataat tggagcgata ttgatatacc tacgggacaa ttttatcatg
     7681 tcaatcttga taacgactgg ccttatggcg tatatggtgc acaacaagat aattccagca
     7741 ttaaaataaa atccagaaca agtggttata gcatcggagc gtccgactgg catccggttg
     7801 cgggtggtga agccggctat attgttcccg acccaaaaga ctcacgcatt acctatggcg
     7861 gcgaatatga tggtattttt tcatcctata acgaagcaaa tgaagtttat cgcattattt
     7921 ctgtagaccc cgaacaacat tatggtgcag gagcgataac ggcgcagcga cgatttaact
     7981 ggactttccc tatcgcgttt tcccctcaca acaacagctg tttgtatgcc acaagtaatt
     8041 ttgtgcatag gagttatgat gcgggcgtta catgggaaac aatttctccc gaccttacaa
     8101 gaaacgaccc gaaaacatta caaacgagtg gcggcgatat tactttagat aatacagggg
     8161 ttgaatttta cggcacgatt ttcacgtttg ccgaatcgcc tgtgaaagcc ggtgttttat
     8221 ggacaggaag cgatgatggc cttgtgcatg tatcaaccga caatggcaca acatggacaa
     8281 atgttacacc aagtaatcta cccgaatgga gtatgatcag ttatgtagag ccttcttatt
     8341 ttgatgccgg tacatgttat ttgtctgcaa caagatacaa gttagatgac atgcagccat
     8401 acatttacaa aacaaaagat tatggtaaaa cctgggagaa aattgtcaac ggattgccgg
     8461 caaatatgta caaccgttgt gttcgcaacg acccgaatgt acagggaatt ttatatgcag
     8521 gattagaaac aggtgtttgg tattcattaa atgatggcgc attctggcag ccattgcaat
     8581 taaatttacc attggctccg gttaccgata ttcgcattga gccaaaagag cgcgatatcg
     8641 taattgcaac gcacggaaga agtttctgga ttcttgatga cataacgcca ttatatgatc
     8701 ttgcgaaaaa tggtgatgct attcaaaaaa gcaacgcata tctatatcct gtaaatacag
     8761 gttatcgcac tcccggccca tcctattatg ctgaggaagc gagcgatcag accggagaga
     8821 atgaagcagt gggcgttaaa attcgatatt atctaaaaca aaaaagcgaa aaagaattgc
     8881 gtttggcgtt ttatgaaagc gatggcgatt taattattac ttatagcaat ttatatgata
     8941 aaaaacgcac gccgataacc atcaaagatg aattttatcc aaatccgaaa gttacggaat
     9001 caggaaaatt aaatgcattt cccggtagtc accagtttgt gtgggacatg cgatatccgg
     9061 atgcaacagg tgataccagt gcaacatttg aagcctcttt gatgggacca cttgccgtgc
     9121 cgggaaatta tatggtgaga atgtacattg gcgattcact ggtaggtcag caaacatttg
     9181 taatagaccg tgatccaaga aacccggcaa ccgttgcaga cttacaggca caattcgata
     9241 tgaaccaacg catttgtaat aaactcaatc agattgcaga cgcaaccggt tccattcgag
     9301 aaacgcgtga tatgatcaat gaacatattg gcgaagacgc ttcggaagaa attaaatcaa
     9361 aaggaaaaca actgattgat gctttgactg ttattgaagg agaattatat aatcctaaaa
     9421 ttctggcata cgaagacaat ttaaaatttc ctatcatgct ggaagaaaaa cttgcgggat
     9481 taaattactt cttgcagatg gcagataccg ccccaacaaa aagcatgtat gccaaatacg
     9541 aggatttaaa tgcgcgcatc gatgtgcaac tcaatgcact gaataaattg atgagcactg
     9601 aagtaaaaca gttcacacaa atggttggag aataagacat ctagggccaa tttgtattca
     9661 tttttgggcc ttataaggct tcttttacgg ttccttttga tggatttgca gaagaagtgt
     9721 cgccagtcaa aacccttatt tagcagcatt ttgagccgat tttaatgtct atacagactt
     9781 caaaacctgt cggcaccttg gaaatgagtc atttttgtat ttgaaggggg tgtaatgtcg
     9841 ttgcattttt gtttcatcaa aacaacaacg ccatgttaca tcctgaaatt ctcagacaaa
     9901 ttttacccgg attagttact tcagttgcta tacgatttcc ggttgcctat atccgcagca
     9961 ttacatttca tgtacatgaa aaaatggtag ctgcctgttt ctggcaacaa gaggagcaac
    10021 gcgctgcgcc atttacaata ggagaaaata atatgcgttc gggatgttac atctacaact
    10081 atgatgaaaa cattcgtgaa agcgaagtag aaataacagg gatggatgaa agtggtaaag
    10141 cgtttttaat ttctatgcct tataatcgca tagtaccaat gatagcagcc tgatcagcga
    10201 tcttcggggg gatttttttc aacccacatt ttattaaacg atttcggggc aaacttgaac
    10261 attgcgcggc gctttcccca agagtttttg aaaagcatat tcacacccat attttttatt
    10321 gccggcgatg gcttatccat cgattttctg ttcaacatag aacgacgcca cattttccag
    10381 gccattttct cgctgaaagt accatgacct tcttccactg cttcatttct gttatacagt
    10441 aataaagtgt ggagatcgat atgaacagga catacttctg tgcaattacc acatagagaa
    10501 cttgcattgc tcaggtgtct gaattcttcc atacccttga aatgcggggt aataatagaa
    10561 cctattggtc ccgaataagt agcaccatat gtatgcccgc cgatgttttt ataaacaggg
    10621 caagcattca aacaagatcc acagcgaatg caatacaatg cttcacgttg cttttcttcg
    10681 gcaagtaatt tacttcgtcc gttatcgagt aaaataacat acatttcttc cggcccgtca
    10741 gcttcaactt cttttttggg tccggtgaaa atggtattat acactgttac tttttgccct
    10801 gtaccataag ttgccaatag cggccacaat aacgcaaggt cattaacgcc tgtcagcatt
    10861 ttttcaatgc ccacaactac gatatgtgtt ttcggaaaac tggtgctgag tcggccattg
    10921 ccttcatttt cggtaacagc tatacccccg atatcaggaa gaataaaatt ggcgccacta
    10981 aaacccacat cggccttcac atatttttcg cgcaaatttc tgcgggcaac caatgttaat
    11041 tcctgagcag ttaggtcgag gctggtacca agtttttcat tgaataattt cgctacatca
    11101 tctttgcttt tgtgcattgc cggcgtaacg atatgatagg gcgcttcccc atctaattgc
    11161 tgaatatatt ctccaagatc tgtttctaca ctttcaattc catgttcggc caggaaactg
    11221 ttaagatgaa tctcttcggt tgccatgctt tttgccttca ccgccatgcg cgcttctttt
    11281 ttcttcagga ttttgagaat ttcctgttgc gcttcttttt catcgttggc ccagattact
    11341 ttacctccgt ttgcggtgaa atttgattca aactcgataa gatatttatc gagattctcc
    11401 atcaccttcc acttgaggtt ttttactttt ttccgcacat gctccagatc ggcatattgc
    11461 tttttaccct gaacaacagt agcattgtac ttgccgatat tgaacaacaa ttttcttttg
    11521 tgttcagcat caaaagcttt ttgttctgct ttttgcaaaa atgtattcag attgcctgac
    11581 atgctgccgg tggttgtcac aaaaataact tatctgacca taactttctt tgacggattg
    11641 tgtaaaaaca tcctctgtgt gtatttctgc atacaattca ctcatttata cctttagcac
    11701 cttgcttgat agccactatt ttcaatacct tcacctctat gaagcagaaa atacttcttc
    11761 ttagcgtgct tttgacgatc gttcctttaa gcacatatgc cggcacaact tccaatggtc
    11821 ccacatttgc cggagtgccg tatgagttca ttctattcgg attgacactg ctcggaatcg
    11881 ccctctttca taaatacacg ctaaaaatcg ctctcagtgg gcttgcggcg gttcttgtct
    11941 ataaaatttt cgtcctcaat tttcatgtta ttgagcattt ggggcacgaa tgggagatca
    12001 tgctcaatct gttagggttg ttgctgggct ttgccatact agccaaactg tttgaagact
    12061 cacatattcc gaaacgcatt cccaaatttt tacctcacga ttggcgtgga ccatttattc
    12121 tgctttgttt tgttttcgta ttaagcagct ttctcgacaa tattgctgct gctttaatag
    12181 gcggaagtat tgcgcatgta gtatttaggg gaaaagtgca catcggatat cttgccgcta
    12241 tagttgccgc cagtaatgga ggtggaagtg gatcggtgct tggcgacacc acaacaacta
    12301 tgatgtggat agatggtgtt gacccgagga atgtgattca tgcctatatg gcagccatac
    12361 ctgcatttct tttcttcggt gtttttggag cattattgca agacaaatac catcccatgc
    12421 tgaagggaag ctacgatcca cctaaaatag atctgaaaaa attatttgtt tgtatttgta
    12481 ttttaggagg cgccatagct gcaaatttca tcctcgattt tccggcagca ggtgtttgga
    12541 ttgctataat tcttggtgcg cttattacta aaattgattg gaatgagctt cagaaggcca
    12601 ttcccggctc aattttcctg ttgagtctgg tattaatcgc ttctatgatg cctgttgatg
    12661 cgcttcctga tgccacctgg caaacaacta tggtcttggg atttgtttcc agcgtttttg
    12721 ataatattcc acttacaaaa ctggcattgg atcagggtgg ttatgattgg ggatttttag
    12781 cctatgccgt tggatttggt ggcagtatgg tgtggttcgg atcgtcggca ggcgttgcca
    12841 tttcaaatat gtatcctgag gcgcgctctg taggcagctg ggtgaaaaat ggttggtatg
    12901 taattgtagg gtatgtaatt ggatatttta ttttgttgct cacactcggc tggaacccgc
    12961 atcctcctca taaacctccg gttaacagta ctactacttc ttcttcccaa atagattaaa
    13021 aaaagccgga aagaaacaat tcttcccggc tataaaaaac aaccactact ctaattatat
    13081 ttctgtttag ccgttcagct aaaaatttat ttttcgaatt ataacgatgt ggtatttacg
    13141 ggagcatcag ctttaattga aacaccaaac catattaaat cctgtataag gtcatcaggc
    13201 atacccttat aaacaatacc ccattgtttg cgatcgatat taaatttagc aacagctgaa
    13261 acgcctgaat tgttcactgt taccttggca gggaattcaa tattcttagt tacatcttta
    13321 atagtaagat tggcggatat catgtagttt ggatccgcta ccgtatattc attgatctca
    13381 ggttgatctc ctccagttga tgctcctgtt gtatttggtt tcacagaagt gatctccaat
    13441 tttgcttccg gaaattttgc aacatcaaag aaatcaggcg attgcaaatg gcctgtaagc
    13501 ttgccattgg tcatatcatc gtctttcaca gatttcaagg tgggcatatc tattacgaag
    13561 ttaccaccgg tgattacgcc atcctttaac aataaagtac ccgatttgat tgctactgtg
    13621 ccattgtggt aagtcgacat cttagtccct atccattcga gcgaactcgt tgcgggattt
    13681 atgtttactg aggtagaccc ctctgtcaat gtcacttcct gtgcgtcgcc ggttaaggct
    13741 tcatcgctgt ccggagcgtt cttacatgcc gaaacgctca ataataccgc tcctaagagg
    13801 gcaatagatg tattcttctt cattgatttt atcaaatttg tatgtatata catacatttt
    13861 aacaccaaag ttcccaaaag gtttacgatg gttaaacatt tttattgaat ggcggttatt
    13921 tgcatattca gggcgtatat ttgaatgaaa tacaggcgat gattaccaca ttactcactt
    13981 ccttatgtat agccatgctt tttatgaccg gcgcatggtt gtttagcttg tacaagcgcg
    14041 acatgagtgt ggttgacgga ttttggggcc tgggatttct agccattgcc caccattggt
    14101 tcttcagatt ggaggaggcg cagttgcgac aatttgcagt agtgtatctg gttaccatat
    14161 ggggattgcg cttgtcggca catattttca tccgcagtaa aggcaagccg gaagacccca
    14221 gatataccgc atttagaaac gaatggaaag aaaacacctg gtggatcagt tatttcaaag
    14281 tatttctttt gcaagcggta ttgtgttttg tgatttcatt accggtaata aatgtactgg
    14341 cgcatcacga ggagggattc agctgcttaa acgttgttgg gttgattctg tggtttatag
    14401 ggtttgcctt tgaaagcact gccgattatc aattattccg atttaaaaag aatcctgcca
    14461 ataaaggaca ggtaatgaat aagggtttgt ggtacttttc gcgtcacccc aattatttcg
    14521 gtgaagtatg tgtttggtgg ggaatatttc ttgtgagcat aggttccgga ctttggtata
    14581 tttccgtttt agggccaata ctgattacat ttttattgct tcgcgttagc ggtatcacca
    14641 tgttggagaa aagatatgaa ggcaatgatg cttattcttt atacaaaaag aacacatctc
    14701 ctttctttcc gtggtggccg aaaaaataat tggctacaat ccacgttttt tatcgatgcg
    14761 cttgctgctt ataagcctat ttctaccgtg gagtttcaat ttatatgctc aatgtgatcc
    14821 tgtgtataaa cgcgaagtaa ttgccgacta tttatatccc gacttcaatc tcgatttaat
    14881 atccaattac acaatcgaca gtgcaggaat tcaattaaca atgcgcatga atgccgacct
    14941 tacttggaag catattaccg acacccttgg cacaagtcgc tctgttttgc atactcgata
    15001 tgcatactat cctgaggcta aaagctttat tgaaaccgca ttcagtaaag ataccttgcc
    15061 taatatgggc gaaggcgtgt tggaatcaag cttggtaaca ccacaagggg cgcttactct
    15121 ttattttaca catgacaaaa aacacaataa tcgattatat atttatcatg ctgaagagaa
    15181 cgccatttac ataaaagatg tgccattcgg atatgagata ggccaaaatg atacaagtgt
    15241 attttatcgc acaacgaaat tcaagcatga aaaagtaaaa tatgccgcag gaactattgt
    15301 ctctgtagga ataaatgaca gcaaaatcga gcagccttta tttaaacatc aaagcgaccg
    15361 ctttcaacac tgtacatttt atgccgatcc ctacattgca tattggcaac tgtacagcca
    15421 atctttctat gattgccata ccgcctttca caataaaatc tacatacatt acaacaactc
    15481 actgaccgat agcattcaat atgtagatga atatattaaa ccatatttgt cgttaaaaaa
    15541 cgatacactt agccttgtta tttacgaatt tgatacatta cttctgaaat catttttatc
    15601 gctatatcaa ctcacatccg gtcaatttat tcgcagttgg aaaagtcaag aatggcagga
    15661 agtggttaat gagaatttct catttattaa aaactatcga attgtgggta gcgtcatttt
    15721 tgtcgaaaca gaaagacgtg tttcttccga ggatgtcaca aaatcgatcg aagttcccta
    15781 tgtactatct ccaaatggct tgccctttac caaaaagggt acaatcccca ttattacggc
    15841 cttcgatttg cacagcaatg cctttttagg ttatccgcaa attagcatca tcaattaatt
    15901 gggtttgggc atattttgct ctgtcatttt ttgttataaa gtgaaatact attgacaatt
    15961 gatttgcatt tgttaacata ttgaaagcgc aggaattatt agtttagcct ctaatttcca
    16021 taaaacatct cctcaccatt aatttgcaac atgtctacag taaaaaaaac gattgttcct
    16081 gttttgctta gcactgtttg gatcagtgtt aatgagttta tcaggaatca gcttgtatta
    16141 caaaattatt ggaccgatca ttataacaac ctgggcatca cctttcctgc ggagccggtc
    16201 aacggtgcgg tttggggtat ttggtcttta ttatttgctg tcgctattct catcatatcg
    16261 aaacgatttt cgttgcgcga cacaacacta ttgtcgtggt tggttggatt tgtattgatg
    16321 tgggtggtga taggcaatat gggtgtactg ccaacgggaa tcttgatttt tgccattcca
    16381 tggagccttg tggaggcatt cggagcggca tatatttctc attattttac taagccgaga
    16441 gcctgatgct gagtgcaaca gccgcttccg ccatattagc ttatggtggc gaaacctttt
    16501 ggaaggaagc gaaaaatatt gaggccgaag tgagtgtccg cggatgggca tttcgcttaa
    16561 agtggcgtcc gtttttcgat catgtgcata tcaaattaga tgccgaacat ccattttcac
    16621 aacttacacc tattggaagc gaccttggtg tggtggcagt attagatggc gacgacgtgc
    16681 accttgaaga cctcaccggc gaaatttcta aattcagatc cggggcgaaa cattatttcc
    16741 catacggcag gagattattg tattgggatg atctcgatat gggttatttt gcgaattatg
    16801 ctttctggaa ttatttcacc cttccgaaat tattgctgcg cgatgatatt aaatggatag
    16861 aaactaaata cggtgtttta catgccattt ttcccgacca catcccaaca cattgcaaag
    16921 aacaacaatt cacattcgac acaacaacag gcttattaaa acaacacgat tacaccgccg
    16981 aagttatttc cggacttgcc actgctgcac atgtaataca tgaacatcaa acaaaaaacg
    17041 gcatcacatt tgcatcgaga agaattgtaa cgccacgcac taaaacagga aaagccatga
    17101 aagcaccggt attgatcgac attaccgtgc atgatctgaa gatcagttga aattaatctt
    17161 taacaatacc cgaagaaatc agatattccg caatttgtac ggcattggtt gctgcgcctt
    17221 ttcgaagatt atccgatacc acccagaagt ttaatgtgtt tgcttgtgta taatcgcggc
    17281 gtattcttcc cacaaacact tcatcctttc cttcagcatt aattggcatg ggataaacat
    17341 tgtttacaag atcgtcttcc accactacac cttcagtttg ttgcaacgta gtgataactt
    17401 catctattga aaaatcgcgt tcaaattcaa cattcacgct ttcactatgt ccgcccgaaa
    17461 ccggtacacg caccgttgtt gcagaaacca tgatattatc tgagccaata atctttcttg
    17521 tttcattgtg cattttcatc tcctctttcg tgtagccgtt ttccataaaa acatctacct
    17581 gaggaatcac atttttatct atctgatgcg gatatgccgg attttcggca acgcgacatc
    17641 cactgctcct ttcgttatac agctgcatca ccccttttgc tccggatccg ctaacagatt
    17701 gataagtgct gacaacgatg cgttttatgc tatataattt gtgcaatgga ttcagtgcca
    17761 ttaccatttg aatggtagaa caattcgggt tggcaattat ttttctgcca tatttaacaa
    17821 tatcatgcgc attaatttca ggaaccacta atgcacaggt tggatccatg cgccaggcac
    17881 ttgaattgtc aatcacccag cagccaactt cctcaaattt cggcgcccac tccaatgaag
    17941 tattacctcc ggcgctaaag agggcaattt gcggacgcat ggcaatagca gtttccatgc
    18001 ctaccacttc cacatcttca cccctgaatt tcactttctt gcccacggag cgctcggttg
    18061 caaccggtat catttcggta acaggaaagt ttctttcttc cagaattcgg cgcatcatct
    18121 gccctaccag gcccgtggca ccaactacgg caatcttcat ggcacaaaga taaatgcccg
    18181 tttcaggttg ccctctattc ctataaagat tttctgcatc cggggcatca atttaacgaa
    18241 tccggctttt tcaagccatt tatgtaactt tcgggtcttt atttgccatg aagtcgatat
    18301 acacactgtg tttggtgatg ctggcctgtg cagttgggca agcacaatca ttttttgatg
    18361 attttaatga tgccgacatt tccgattggg gcggaacacc cgaatatttt attgccaatg
    18421 gcaccggaga gctgcaacta aatggcgatt gcgctgcagg tggcgatcat tatatcagcc
    18481 aggctttgcc taccctcgac tctgccgtat ggtcgtttca ggtgcatctc gatttcgatc
    18541 cgtctaattc aaatcatacc cgcgttttcc tgcaaagtga tattgccgat ttaagcggag
    18601 atgtttacgg atattttgtt cgcgttggcg aagacggcac cggcgatgtt gtaaaactat
    18661 ggcgcagcaa tggtgcctct gcaagtgtgg tgattacagg aacttctccg gttgcatttt
    18721 ctcctgtagt tggtgtaaag gttgttagaa ccgctgatgc cgaatggcaa ttatatgttg
    18781 atgcggatgg cggaacagat tatgtgttgg aaggaacaga tattgacgat acctacaacg
    18841 gaggaaatta tgcgggcata gaatgcagat atacctctac gcgctgtacg cttttttatt
    18901 ttgatgattt ctcaatcgac ccgctatata tagatgttac tgctcccgca attcaatcgg
    18961 taaccgtatt atcatctaca caattacttg tcgacttcac agaaaacata acactggtaa
    19021 ctgcagaaac cgaatccaat tataccgtaa caggtggcgt aggaagccca attgacgcta
    19081 cgcgcgatgt aattgataat tctaaagtta cacttacctt tttatcagaa tttcccgaag
    19141 gcattacact tacacttgcc gtaaatggtg ttaccgatga tgcgggaaat atcacgagtg
    19201 gcgctaccgc taatttttct gtttacacta ctcaattata tgatattgtc atcaatgaat
    19261 taatggcaga cccttcaccg cctgttgctt tgcccgaaga tgaattttta gaattatata
    19321 ataccactga tgtagatatc gacatcaccg gatttacgct aagcgatgca tccggtccaa
    19381 gcgatccatt tacatcctat atacttcctg cccacggata tgttattttg tgtgatgatg
    19441 gcgatatttc aagcttttta gcctatagta atgtgcttcc tgttgccgga tttccatcac
    19501 tgaataatga cggcgatgac atgatgcttt ccgatcaaaa cggaattgaa attcatcgcg
    19561 ttacctatac ttccgattgg taccaaaatg caattaaggc tgatggcggc tggacgcttg
    19621 aaatgataga cccggcaaat ccatgtcagg gaattgaaaa ctggatagca tcagatgctg
    19681 ttgaaggtgg tacacccgga gcacaaaact ccgttttcgg cagcaatccc gatctcgtcg
    19741 cgcctgcctt gcttgaagtt tttccgcaaa cactcgatac ccttacgctt actttcagcg
    19801 aagctgttat tggtgaaaac ataatcactg ccgatattca gatcaccgat gaaaccggtg
    19861 cgtcgattgc cattgccgca attattctaa accctgacaa tgatgcaaaa attggcgttg
    19921 tgttaagcag tcccttgtcg ggtggtgtta tctacacttg cacagtaatg cacgcagccg
    19981 attgcagtgg aaatgatatt cttttgttta atacaattac gttcggcatc cccgaacccg
    20041 tttccgccgg cgatattgta atcaacgaaa tattgtttaa tccggttacc aatggcgttg
    20101 attatgttga attatataat cgctccaata aaatcataga ccttagtaca ctgatcatag
    20161 ctgagttaga attgctcgac tcaacagtta tcgatgaatt tgcgtatgta acttctgatg
    20221 gtcaattatt attccccgga aattacattt gtgtcacttc tgatatcgag caggttttgg
    20281 cacaatattt cactgttgac accggaaatt ttgttaagaa caataacctt cccggctata
    20341 acgacaatga aggaattgta gtgttatatg atgctacttt gaacgaaata gatcacctgc
    20401 attatttcga cgattggcac tatgccttgc tggatgatga aaatggcgta tcgcttgagc
    20461 gagtgaatta tgcctttgca acgcaagatg aaaacaactg gcacagcgcc gcatctgatg
    20521 tgcattacgg cacacccggt tatcaaaact ccatgtttgg cgaaattcac gcacaggcaa
    20581 caattggtgt agaatatccg gtatttactc ctgatggcga tggttatcac gatttgttag
    20641 taatcacgta tctcacagag tcggaaggct tcacaggcgc atttacatta tttgatgctc
    20701 agggcagaaa aataaaaacc ctcacaacaa atgaactgct aagtcgcgag ggttttataa
    20761 cctgggatgg cgttactgac gatggcagta aagccaattc cggcatttac attatttatg
    20821 ccgaattatt taataccgaa ggtgttgtag aacgattcaa attaaaatgc accttgttgc
    20881 gcaagcaata attatttcac agtaacgcga cttccttcac tatggctgct gaattcaggg
    20941 gcatacatgc actgaatgct tgtgatacca ttactcatgc ttcctttatg gctaacgcga
    21001 acaggatatt caaacacata tgttcctttg cgtaaataag atataaagaa gttagtgctt
    21061 gcatcgcccg ttgcttcata atatccaagt ccgtcttgcc atttatatga actaattaca
    21121 ttagttggtt caaaaccact tgcccgcata tccttcatgt gcacatattc catatcgcga
    21181 tcgcttttta attcgattcg gacaattact ttgtctccaa tatgcaattc cgttttttca
    21241 tcaaccgcac gcaataccgg acccgaagcg gaattgactt ccagaaacaa ttgctttttc
    21301 agcttcaggt ttgtttctgc tgaagttact ttgtccattt cttcaaaata ttgccaatac
    21361 atcgccccat aactcaataa attatttgca ggtggcgtaa tagtaatatt tcccatggca
    21421 ggattaatat tcgtgcccaa ccatgttgat ttaaaataac ctgttcccaa ttccgtttgc
    21481 tccggtttta cttgcaggtt gccaatggta atatttgcca gctcggtatt tgccaataaa
    21541 tttagtcctc tcgacaacaa ggcataacaa gcttcggcag tggcgcgtgt cgttttccag
    21601 tctgttgttt gtttctggcg caataaccag atttgcaaag catccactgc atttttatca
    21661 ttcgccactt cagcaaacac ttcaatcatt aaggcttgtg tttcgatagg tgcctgatac
    21721 caataatatc ccgcaatatt cgatttccaa tacatcccca tctcctccga tttaatactg
    21781 ttttctttca acgaagccac aatttgctgc gctgttttaa cctggtcgtt gcgattcaat
    21841 attatggcaa tcatgccttt ctgataaagc gaataacttg tccaataagt ttttatctga
    21901 gcaataaaat atgcttttgc ttcagcctca tttttagtga gtggaaatct tttttcatca
    21961 aaataacttg ccgcatataa atattgtaat atcaggttat caggaacata agaggatttc
    22021 tcgaccttgt ttttctcaag tctggtatat gtctcataca tgtttgcaga acaataaggc
    22081 atagcagatc gcaacatagc atctacctcg ccggcagtat tatattctgt tgccgaaagt
    22141 cgctccagat gtgcaagtcc tgttacaata tattgcgtga tatatgggtc atctttatat
    22201 ccgctaaacc aaggccaggc gccatccggc agttgcattt tttgcaattt ttgcaaggca
    22261 gcccgttgct ccgactgcat tttatttaca tcaaaaagca gtgctacacg ttttttcctt
    22321 tcagtttcat tttgcgcctg caacacccat ggcgtttctt caagcatcag tgattttaat
    22381 tcctcatttt tttccagatt ggaaaccaat gagcttggat ttttcttcca gctatcgaaa
    22441 acacgtttga tttcagggtc actgttcaca atatgcgccg caatactatt ggcataaaag
    22501 cggctgaata cctgctcggc acattcgtat ggatattcca tcatataagg caacgcctgc
    22561 actgcatacc aggccggatt tgaggtatat tccaatgtca cgttttgatt gcgaagtgta
    22621 ctgctgttac ctgatttcaa caacttgtcg aatacaaatg atttgctatc gccaccgcgc
    22681 acccataaag gaatagattc ggtaaccaga attttgtttg tcaataccgg caaaccattt
    22741 tcttccccat cactaaacgt ctcgtttccg gctttaacgc gatatacgat ggcgtctatt
    22801 ccttcaggaa catgcacttc ccattgaaca gatgtgctgc cttttgccga tgcggtgaac
    22861 gataccgttg cttgtttatt ttcaaacacc gcgttcaccg gttgcatgtt aacggcatta
    22921 aatagttcca attgcgcgga accgctgatg tctttatcgg tgagatttga aatcttggca
    22981 gcaagcgtaa tgtgatctcc ttcgcgtaaa aatcttggca tattcgcctg tatcatcaat
    23041 tccttttgtg tgatcaccga tgccatggca gaacctgttt gcaagtcttt cgtatgtgca
    23101 tatgccatga acttccatgt tgtaagcgca tccggcatgg tgaatgaaaa gctgacattt
    23161 cccaggctgt cggtctccag ttgaggaaag aaaaaagctg tttcattaaa attattgcgc
    23221 atcggaattt ttcctagcgg cgcaggaact tcttgttggt ttttctcttt taccacttct
    23281 aaatcagcat aaaaaccatt gcccacagcg cctttatttg ttttagtaat ttgcacttcc
    23341 tgcatcatag caggcattaa attatctaac ccttccccgg taacaatgga tcttgacctt
    23401 tggtcaacaa aatcggcagc agctcctata tcataattca aaaattgata gccattcaaa
    23461 gaaaatccaa accaattcaa ttgattgtat tctattgtag gataagtggc gtaataattg
    23521 aaatatccat tatgctgcca tccgtatgat tgtcccatcg cctcagcgga aagcgaggta
    23581 tacaaataat tagcaggata ataaacatcg ccccaataat ttgttctgaa ggcatccaat
    23641 gatgcgtcgt acatagatgc caataattcg gcactcacca tgtcgctctt ttcaccactt
    23701 acttgtattt tccatgtttc cttagcaccc ggttgcaatt tatctctgaa ggtttcaagt
    23761 ttcacttgta actgtttgtt cgtccaagga accgtaatat tatatacttt agaaaattca
    23821 cggccatctt ttatcatcat tacctgaacg ccaatgccac ctctgtcagt ttctgaaacc
    23881 ggaatttcaa ggtccatcat agatacgccg gatttttttc cggcaacttc catcatggaa
    23941 acccattgtg cttttgtagg ttgatgatat cttttgatca gcaccaaagc tttagatcct
    24001 gatgttgagc ttcccacttg cacaatagcg ctatttccgg gctctacgga gttttcgctt
    24061 ttcactgaat gaaaatactc ggggtaagcg gcataagagc catcattcaa caaattgata
    24121 gtgtgttcgc tgcgcactgt ttctccggac ttgtcgagcg cttcaaaaac tacgcgataa
    24181 acgccttgtt taaggccttg tgttggaagt aataccgaat cattctcctt tgtattccat
    24241 gtgtcagatg ccaaagctgc tcctgttttc caacttcctg catccaactc ggcggcatac
    24301 tgatcgcgag gaaattgttt aataaagtcg gcttcactca tcaaatgcaa atccggtgta
    24361 tcccaaaggc gatatcgcat caaacgtttc ggtgcgtcaa tggcaaatat tgttactttt
    24421 ccggtagcag gaagtggctg tccattcaag ttatgggtag ttatttttat tgctatgtca
    24481 ctgttaacat gatatgcatc ttttacatct gcactcaact gcagatttat ttttcccaca
    24541 gtcacatttg tttgactact gcgcgtttct ccggaaacat cggtcacatc aacataaaca
    24601 gtataggtat attgcaatcc cgaaacgttt tctacctgcg atttagctac aaacggtatt
    24661 acaaattttc cttcagcatc tgttgcactt acacctgaaa caatttcctg atcagcatta
    24721 cttacaggat atgatttcct gaaataataa tcgccatacc aatatggcat atgaacactt
    24781 cttaccacgc gatactgtat ttgcgcatta tcaattactg cgccgctata cgccgttgct
    24841 gttccgttta cggaaatggt gtcattaagt atataatctc ctttcaggtc ttctaattca
    24901 acataaaatg tcgggcgctt atattcctcc acagaaaaat acaccgctcc ggtttcattt
    24961 gaaatttgca tttgtccggt caatccgctc gcaggtgctg taaagcttcc cgaaaatgaa
    25021 ccaaaatcat ttgacaccaa ttcaaggttc cctacttcct gataatttac atcaaaaaaa
    25081 cgaacagtgg ttttatgatt cttaagtact tcttttgtac ggccaaatgc tgtttcgctg
    25141 tacatgatac ctttaaaata aaccgtctgt cccggacgat aaattgccct gtcggtaaac
    25201 aaaatggtat gttgaatttc tcgatcaggc tgtgtttgcg atttcgcaat gctaaacagc
    25261 ttatttgttt tcaatatgtc ttttccattc tgcaaagtga atccaaatga ataagtgttc
    25321 ctgattatcg gatcacgcaa ttcaaacatc ccattgccat cggtcacaaa tttctgtaac
    25381 tcactctctt tatatttatg tgtgctataa tcatagtcgt tgcggaacgt ggtaacatta
    25441 acacctttca tcggcaatcc gcttgttctg tcgacaacat aaaatcgctt gtaatcgctt
    25501 tcatcaccat tgccggattc ggcgatgtat gccatatttg ttacccatat atcacggata
    25561 tacacatctg cagaatcgct catccagtta tcattggcag aggcaagcag aaaatatgaa
    25621 ccaagaccaa gtgcgtcaat tttaaattct actctatggc tttgaaggtc gccatcaaca
    25681 attaaatttt gcgtccatga acgttctaca acaagtttct tcagcgccgc ttttctgtcg
    25741 ctataatcca ttgaggatat cgatacaatc tcgttagcag acgctttgag taattgcaga
    25801 tgaatcttat ctacattggt atacttaatt gatgccaata taggtgctcc gggaatttgt
    25861 tcctgctcga tatttaattc ttccaatcgc ttgcgaacaa gctcatttaa ttccagttta
    25921 cataatatgc cgccgtaact tttaggaaat gcagcaattg ccttattcaa gtacgtttca
    25981 gctgtgcgca attcccattt ataatcatcg ccttccaaag gttggtactg atatcttttc
    26041 cccatatgcc aggtagcaat ggcaagcgaa attcttgctg aggagctatc gcgggcatac
    26101 tgtttttcat cgcgctcaag tgcttgcaaa tataaactgt cgacattagc aatcgacagt
    26161 atgtttttag catacaaata tctgctcaga ttaagatcga tcaatgcatc cggatatggg
    26221 tcgtataaat gtgctgttgt aagctcttgc aaaatgcgca ccgcgggcaa ttgtgtgata
    26281 acactgtcag ctgcgttgaa actatatcca gaaaattgtt tcgcagattc cagcaaaatt
    26341 gggttggcga tattcaccgc ataggctggc ttgtctaatg tgctttcgct attcatataa
    26401 aaactcaacg cctcattacc cagcaggtcg aacatggtgg ggcgtaaaaa ctgaccatat
    26461 gcatttcctt tcaaaagaat ttcaacatat aaggttgaag gtgttttttt taataattcg
    26521 cgctgcgaca atgatgcatt gtacagctca tttgttttgt tgagcagcgc tttaatatcc
    26581 caagtagcaa tatcgttgct caataactct tctgtttgtg tgcgttgtga aatttgccag
    26641 cggttattag aataatattg ccagtacaaa tttgctgtca ggctttgcaa taccgctttt
    26701 gttacgccat cagaaatggc tatttctttt tcaaacaatg caattgcttt ttcctcgcca
    26761 tcttcttcca gcatgttggt gtatttccca cgataaatca aagacttaat tatttgagga
    26821 ctaatatttt cggctttggc gtgatcataa atttcattca ccaatttcaa tgcactctca
    26881 ggcatgctaa tccggtccaa cgagtcaacc gctttccagg catcgtcata tcccgcccat
    26941 gtttttgttg gtgtcattac cgctgcaagt aatcctgtca tagccgcaac cgctacaatt
    27001 gctccggcca ctatccattt tttcccttcc ataattattt ctcttattct ataaatgtaa
    27061 taaagtcaag tcttatgtaa ccataagatg catcgccttt ccaattttca catcagcttt
    27121 gtttggcaac ctttcgcaaa tgcatcatca atgcaatatc aatcacggtg tgtgctgtca
    27181 tagcggcata gagtcccgta tgcaaacaca ggtatccgaa tccggcactt accaccaaca
    27241 atatcgtgcc ataaaacatt agcgggcggt ttagcgggtt gaggtagcca tgaagcagga
    27301 tgaaaataaa ggcagtgaac caaattccca gccagggctg aatggcgcct ctgaataaca
    27361 actcttcccc gacgccggca cataacgaca aaatcaggat taccggtgct gagatagcgt
    27421 attgagtgag caattgggca aaaaacgata caaccggttt tagcaaagcc agtttaagca
    27481 cccccaggag ggctgccagt gaaacagccg caaatcccgt gcccaaaagc aactgaaacc
    27541 ccaattcttg ccctgaaacg ctagtgagac gggcaatttc gtcgggatga aaaaagtaca
    27601 tcaaccccaa acccaagcca cctaaaccgg gcaacgtgac aaccgccgcc aggaccagat
    27661 gtcggtgcat ggtcattgct gcacgatgcg gattgtagaa acgcctgttt gtttggaact
    27721 ggtagcatga ccctggtagg agtcgctcac gtcgaggtac tgggtaaatt tcatcaacct
    27781 tggccagccg gtattttcga agatctcgcc cgttcctttg actccggcgt tgaagtgtat
    27841 cttgtccacc tcagcagcca tttcaggcgg cagtttcgat ttgtcgacgc tggcaaccgt
    27901 gttgaaattg atatatgcta tcccgtcgcg acggtcggct agggcgaaaa cggtttttag
    27961 tgttatgggc acaccctgca ttactgatgg gacttccaca tcccaggtat ccccgatttt
    28021 cacggcgctg tcgggatagg aaatgaaaaa atattcaagg ttgtcggttg gtgagccgat
    28081 gtttgctgcg atatattgat cgggcacctg tgcaccaact attgctttta cctttttaat
    28141 gatagcctca taaccacgca catcgatgtt ttcaccctgc tctgtcatta tgagggtgag
    28201 ggtgctattc accatacagt cttcaacctt tctatccatt tcgtcttccg gattcgtcat
    28261 cggtttgttg tactgataat taatggtgcc cgaatcgtct tttaccgttt tgtgcacatc
    28321 aaccagcgtg agatcaatag catagccctc tttttctttg ccctttacaa cgagctttgc
    28381 tttcacatac ttagttgaat ttgccttaat tcgcatggta tcaaccgatt cgtcagaagc
    28441 aaatgttgtg ttgttttcga tataaatcgt ctttccttct tcaggcataa agcgaagtaa
    28501 aacacgctca tctgctacag ggctttcgag gttgattgca atggcggtat cattgcttgc
    28561 agcgacaatg gtgtcggcgc tttgattatc tttatcgcct gatgacgatt tgttgcagga
    28621 agttgaaaat agtgcagcga tcatcagacc ggcaaccaca gcgattctta attttatcat
    28681 ttgcaatgat gcgttgagtt gatgtgaaat tcgcctgcaa agctacatga ataccatgga
    28741 aagtactgcc cgacaagaca aatccttcgc cgaagcctat gcacagctta acgacgcaca
    28801 gcgcagagct gttgatcata tcgaaggtcc ggtgttggtg gtagccggcc cgggaacagg
    28861 taaaacacag atacttgctg tccgaatagg aaaaattctg gcggaaacag atgcgatgcc
    28921 ggagaatatt ctctgtctta catacaccga tgcgggcact gttgccatgc gcaaacgttt
    28981 gctcgatttc attggtgccg atgcacatcg cgtacacatt catacatttc acgccttttg
    29041 caatgatgta atacaagcgc atctcgatta tttcggaaag cgcgaattag agcccattac
    29101 cgatctggaa aatgtgatga tattggaatc aattttagaa gaacttccta cgggccatct
    29161 gcttaaaaaa ctgaagggcg atcttagcta tgaagtagcg cgattgaaca acctttttcg
    29221 catgatgaag gaggaggatt gggatgaaaa gcgcattagt gataatatcg atgtatatct
    29281 tgccgatctt ccgaatagaa cggaatacac ttaccaacgt gtaaacaaca aaaccggcgt
    29341 taaagttggt gatccgaaag taagtaagat tgcagaagaa caggaacgca tggaaaaact
    29401 gagagccgct gcacagttgt ttccaaaata tcaggcacgc atgaaagaag ccggtcgcta
    29461 cgattacagc gacatgatct tatgggtagt gcgtgcattt cgcgacccat ccgccgtggg
    29521 cgaaaacatg ttgcgcaatt atcaggagcg ttacctatat attttagttg atgaatttca
    29581 agataccaac ggcgcacaaa atgaaatcct tcaattatta attagtttct gggaggtgcc
    29641 gaatgtattt gccgttggcg acgacgatca aagtatttat gaatttcaag gtgcgcgtgt
    29701 aaaaaatatt ctcgatttct ataccgcata tcaggagcat gttgaagtga tcgtgctcac
    29761 agataattat cgctcgcatc aggcggtatt aaatgctgcg cgagtggtga ttgaacaaaa
    29821 tcaagagcgg cttatcaata agatcgaggg cttgaccaaa cagttaacgg catcacaccc
    29881 gtcgcgacaa aaaattgatg agcctaaagt tattgaatac gaaaacccca ctcaagagca
    29941 tgccggaatt ttacgtgaag tagaaacgct gctaaagcag ggagtaaatc cggaagaaat
    30001 agccatatta tatcacagac acaaacatgc tgatgatctg ataactgctt gcgaaaaacg
    30061 gggaattcca tatcaggtaa gaaagaaaat aaatattctt gaagtgccca ttgtgcagca
    30121 gatgctcacg atgttgcgat atttaaatga agaggcgcaa tcggcacaca gcgcagagca
    30181 tctgttgttc gagatcatgc actatccatt tattggcata catccacatg atgtagcggc
    30241 attatcggca tggatatctt cacgtcgcga cccggaaatt acctggcgca cggtaatggc
    30301 tgacccaagt aagttgggac aaatacgatt gcgcgcacat gagtcaataa caaatctgga
    30361 aaaaaatatc acccactgga tacaggaaac atttaatctg acactgcaaa tgttgtttga
    30421 aaaggtgttg aatgaaagcg ggttgatgca gtatattctt aagaacaacg acagagtgtt
    30481 tctattagag gcaatcagca ctttattcaa gcatattaaa gctgaaggaa tgaatcgccc
    30541 gcgcattacc attcgcaatt atatggaggt gcttgatcaa atggagacac atggtattca
    30601 attgtcgatt gagaaaacta ttgcacaaaa aggcggagtg caattcatta caacacatag
    30661 cagcaagggc ttggaatttg aatatgtgtt tttattggga tgcaatgcat ctaattggga
    30721 gaagtcgcgc ggagataatc gcatgtttgc catgccggac accttgacca tgagcggaga
    30781 ggaaaacaaa gtagaatcgc ttcgacgctt gttctatgtt gctatgacaa gagcaaaaca
    30841 acatctgtat atttcatatg cagctgcaag taatgaagga aaagtattgg aggcctcgca
    30901 atttgtttct gaattattgg caggtgaaaa catttccgtt gaaaaatgta atatcgatgc
    30961 agaaacaatg atggcatatc aactaacatc attatcacca acgaaagaag tgcatattga
    31021 tttatttgat aaagatttta ttgcttcaaa ggttgagcat tttgtgatga gtgcgagtgc
    31081 attgaatact tacctagatt gtcctttgcg ctattatttt gaacgtatta taggcgttcc
    31141 cggagcaaag aacgatagca tagcctttgg taatgccgtg cattatgctt tgcgcaagct
    31201 gtttgaacgg atgagggccg atgagcgtca gcaatttcct ccggaatcgg tgtttatcag
    31261 cgatttcatg cacgagatgg agcggaatga agatgcgttt accgaaaaac aatttaataa
    31321 tcgcatggaa ttgggtaata cgcttttgag cgattattat cagacgcata ttcatcagtt
    31381 caataaagtt gtggttacgg aataccccat tcgcaatgcg gaaatggagg gcattccgat
    31441 caccggcgta tttgataaaa ttgagtttat aggaaaagat gtcaatgtag ttgattataa
    31501 aaccggcgct gtaaaatatg cgcgtgaaaa attacttccg ccaaacgaaa aaaatccgga
    31561 tggcggcgat tattggcgac aaatcatgtt ttataaaata ttgctggaca gtcagcgcac
    31621 aaaaccctgg aagatgatca gcggcgaggt tgactttatt gaaaaagacg agagcggtga
    31681 atttgtgcga cataaatttg caattgcctc agaccaagtt gacatagtta aagcacagat
    31741 ccgcgacacg tatgcgcgca taaaaaacct agaattcaca acaggctgtg gcgacgacga
    31801 ttgcgattgg tgtaattttg taagaaatct tgagcaataa ctttcttctt aggcataatt
    31861 tattattgat tattcggtgt aaaataatca gtatatttat ttcattaata cttctttaaa
    31921 acattttgca atgcaagctg cggtttatac ttcttatggg gctccggatg ttgtaacggt
    31981 tcgcgacatc cccacaccac gacccggtga aaaagatgta ttaatccgtg tgcatgcatc
    32041 taccgtaaac aggacagatt gtggattcag aagtgccgaa tattttattt ccagattttg
    32101 gagcggctta tttaaaccaa aacgccaggt acttgggtgt gaatttgcgg gcgtggtaga
    32161 ttctgttgga agcagtgtga ctatgtttaa acccggcgac aaagtattcg gattcaacga
    32221 tcgcacattt ggcgggcatg ctgaatatat ttgtcagccc gaaaacagtg caatggatat
    32281 tattcccgag catttaaatt ttgagcaggc ggccgcactt acagaaggct cacattatgc
    32341 attaaatgac atttatgcgg cacatgttaa agccggcgat catgttttag tttatggtgc
    32401 aacaggcgca atcggatccg cagcggtgca gatactaaaa tatttgaatg cacatgttac
    32461 cgcagtttgt ggaacggcac atgtagatct gattaaatct ttaggcgctg atgttgttat
    32521 cgattatatg caacaggatt acacgcaaca aggattgact tttgatttgg tttttgatgc
    32581 cgtgggaaaa acatcatttg gcacatgtaa acctgtatta aacgagccgg gaatttatat
    32641 cagcactgaa ttgggaaaaa acggtcagaa tgtgtggtat tccattttca atgcatttac
    32701 cggaaataaa aaagtgcttt ttccgcttcc cgtttacgac aaagaaatga taagttttat
    32761 tcgcaaaata gccgatgaaa aacattttac acctgttttg gataagactt atgcgttgaa
    32821 agatattcgt gaagcatacg cgtatgttga aaccggaacc aaggttggca acgtgattat
    32881 ccgctgcata taatatcgct ttgcaagcat gcattgtgta catttgtgtg attaatattt
    32941 cattttcatg gcgaacaatt attcagcatt tacagcacac ccctggcatg gtattcacat
    33001 tggcgacaaa gcgcctgagg tggtgaatgc atacattgaa attgttccta ccgatacggt
    33061 gaaatatgaa attgacaagc caagtggttt tttgaaaatc gacaggcccc aaaaatattc
    33121 gaatgtgttg ccttgtttgt atggctttgt tccaaaaaca tattgcggcc ccggtatcgg
    33181 aaatttttgt tcacaacaaa ccggaagagc gggaattgtg ggcgacggcg accccttgga
    33241 tatctgtgtt tttactgaac gcaatattcc gcatggcaac ataatcgtgc cggctaagct
    33301 catcggtggt ttcaggatga tcgatggcgg tcaggctgat gacaagatca ttgccgtttt
    33361 gccttttgac gagatttact ccatgtggga atacattcaa caaatgccaa gctctttggt
    33421 tgaaaggctg agacactatt tccttaccta caaggccctt cctggagctc cgaataaggc
    33481 ggagattacc catttatacg gtcgggaaga agctataagg attcttgagc ttgcccgaaa
    33541 ggattatgcc gaattgataa aatagtgctt acgccatagc catattttta gcaccacttc
    33601 tatgcactta agcagctctc gactgcgcgt tttttctttt ttatgccgat ggtgaccggt
    33661 atttcctttt gtaatcgaag ggttgtgtaa tttcgtgacg ttgagttaac cataatcaga
    33721 taatatcatg ggtgagaaag cgagtttata tgcgccgcta ttggtcgaaa cggcttggga
    33781 agtatgtaat caggtaggtg gaatttatac tgttgtgcgg agtaaggctc ctgcaatagt
    33841 agacaagtgg tcgggaaact attgtatgat aggaccactg cttaatgcca agcttcctgc
    33901 cgaatttgaa gaggtattct catacgacga tgtttttgtc gaagcggtaa cggcaatgcg
    33961 taacaaagga tatgaagtac attatggtcg ctggctaaca gatggaaaac cgaaagtgat
    34021 attgatgtcg cccatgcaag tgtacgacaa aatgaaagac attcgcaccg atttattgcg
    34081 cgaccacaaa atcaacattt ttaatccgga tgatttgtta caacaggtca tcgccttcgg
    34141 gtatgtggtt gctcaattct tcagcacatt agaagcaacg gcaaaaggtc gccccgtaat
    34201 tgcgcatttt catgagtgga tgaccggtat gccgatagga catattcgca aagaagggtt
    34261 aaaagtaaaa acggtattta caacacatgc aactttattg ggaagatata ttgcgggtaa
    34321 tgatgaacaa ttttatcggc atttgagcaa ttacgactgg ttgcagatgg cacaaaaatt
    34381 ctatatcgaa ccacaaacgc gattagaaag aatggctgcc gcagattgtg atgtgtttac
    34441 tacagtaagc gatatcaccg gaaaagaatg tgaattttta ttgggacgaa aaccggatat
    34501 gattttaccg aatggtatca gcttaacacg atttcttgca acacatgaat tacaaaattt
    34561 gcatggtcaa ttccgagata agattgatga atttgtgatg tcgcactttt tccattctta
    34621 caccttcgat ctcaatcaaa cgatatattt cttcacctcg ggaagatatg aatatcacaa
    34681 caaaggatat gatttaacat tagaagcgct gcgcagatta aatgagcgca tgaaaaaaga
    34741 aaatgcgcag aaaacggttg tgatgttttt tattaccaaa cgtcccttcg aaacaattaa
    34801 cccgcttgtg ctgcacgccc gcgccgcaat ggacgatatc cgcagcactt gtgaagagat
    34861 gttgaaacaa gtgaaagata aattatttca cgccgccgct accaatgtag atccaaacgt
    34921 tccggatctg aatgaattta tcgatgatta ttggaaattc agatatcgaa aacaaattca
    34981 aggatggaag agcgatttct ggcctattgt tgtaacacat aatttaaagg acgatggtgg
    35041 tgatgagata ttaaattact tgcgtagcaa tgacctcatc aacaaaccaa ccgacaaagt
    35101 gaaaatggtt tatcatccgg attttgttgc tcctacatcg ccgttatttg gaatggatta
    35161 tccgcaattt gttcgcggtt gtcatttggg attatttcca agctattatg aaccatgggg
    35221 gtatacacca ttggaatgtg ttgcgcgtgg ggttccggct gttaccagcg atttaagtgg
    35281 atttggaaat tactgtcaat caaaattccg caacctcgat gaacgcggga tctatatcgt
    35341 acgcagaaaa acaaaaacct ttgatgaaag tgctgatgag ttgtgcgata tcatgtttaa
    35401 ctatacacaa ctcgcccgtc gcgacagaat tattttaaga aacacggtag aaagttatgc
    35461 cgaacatttc gactggagta atctcgcagc taattacgag aaggcatacc agatggtgtt
    35521 gcaacaggag tgagggaatg tgagaattag caagtttgag aattagcaaa ttagcaagtg
    35581 tggagtgctt ttaacgctgg cgcgttaata gggaatgcaa tgatattgct ttgcaatatt
    35641 ttaattccga agcgcaatca gctccctctc catctcctac cgtcgataga gagggcgggg
    35701 ggtgaggcga gaattagcaa attagcaagt ggtggtgctt ttaacgctgg cgcgttaata
    35761 gggaatgcaa ttcaattgct aaataaaatt taattctgaa acgcaattag ctccctctct
    35821 atcgacggta ggagatggag agggcggggg gtgaggcggg gccgggggtg aggcggtttc
    35881 gcgcaaagtc gcaaagagac taaggtgcaa aagtctagat actagatact tgattctaga
    35941 tactaaaaga gagttagcat gtgttcaaag gattcatttt cgcaaagtcg agtcaagatt
    36001 accacccgcc tactcggcgc ggtaaactca cccactgacc cactcaccca ctctctcctt
    36061 tttcctctct cctttttcct ataaaaaaaa cccgccttca aacgaagacg ggttttttat
    36121 ttcgttttgc taattattag agcttcacga tgttacctga agtaacgctt gaaccggcaa
    36181 caacatggta aacatagtta cctgcaggaa ggttgctcat atctaaagaa tatgagtggt
    36241 tacctgctga ttgagtacca ttgttaacag tcaatacttc ttgacccatc aggttgtaaa
    36301 caccgatagt tacatcagat gcttcgctga ggttgaagtt gatgttagtg ttggttacag
    36361 ttgggttagg tgaaactgaa agaccgctga tagcattgaa atcttcaacg cttaatacaa
    36421 caggatcaga accgtcgcaa cggtagcaag catcccagca gaacaaatac ataatgtcgg
    36481 catcaggaat aaccaatgta cggttaacgt tacctgcgcc atcatcaaca ccgcaaccgt
    36541 cagcaccaat agtgttgcct gcgtcagttc cttcgttagt accccagtcg ttgttcacga
    36601 atttgtagta ctgagtaagg ccaactgaag ctgttggata agttacagtg ataccccata
    36661 cgttaccacc catgtcaacc aatgcacatg ttggatctga aggagtccat actgccatag
    36721 ctgcagcacc gttcatacct gcagtagcgc cgaagtcacc gccaacacga ataccgttgg
    36781 cacccaatac gttacctgct gccaggtagt ttgtaatgtc aaccatgtag gtcacgttta
    36841 cttgtgctgt aagagcaccg gtgatcaaca gagcagtgaa aaaagcgtaa atttttttca
    36901 taagatcgtt gtttgtttta ctttaagaaa tgaatttgtc aggataaagg tactgctttt
    36961 tattgcattt gaagccaaat ttttaacatg aaaatgaccg ttgattaata tgttaaacac
    37021 ttaacgccgg ttggtgatat ttatcaattc aaacgctgaa ttttttccca attctgcccc
    37081 atatgttact gccggaacaa aatgaattcc gccgtaaacg cggctctgag atgcctcaga
    37141 agcagcatcc tcaaaggatt tgaaccctct tgccggcagg ccgaatgtgg tttcagttgc
    37201 gtctgtaaat gcaaaattgt cgccaaataa tgaagtaagg attgttgcag cggcggcgga
    37261 aactgcgcta tgcccactcg gaaattcggg gaagggtggg gtttgtaaat atggcctcca
    37321 ttccgaatcg atatatgtat tgatatatgt ttcaggacga atcagttgcg tttcatattt
    37381 aatatcccaa acagcaatca tggcatctgc cattgctata ctaaccaaca tatacgcatt
    37441 catggcggca gtctcatcgg cacctgttgc cagaagcgcc attctggtta tgtctaacca
    37501 gtgtccaggc ggactcacct ttttttcagc atattgtaaa tgtccgttct gacgaagaat
    37561 aaatggattg tcatcccaga atagtgcaat ttccatttgt tctgaatcga gagacattgt
    37621 cagtacataa agtttttgag cctcttttgc gataacagtg ctatctaaaa caaacgccgg
    37681 aacaggtaat atgcttttgt cgtacttata acaaaacggc atgatttctc cccaatgtgg
    37741 atctgtacct tgagcataat ccggcggcgt gggcacccag ttgagcgaac cttttaatgg
    37801 tgtatatctg gttgacgcaa tggcattagt gtaattatcg gcattaatcc aatcggccat
    37861 ggaagcatta attgtttgcg cttccataaa atattgcgag gtgataattt cactccccag
    37921 acgctttttg catttctcca atagcatgct gtcggaataa ataaatcgct gtgcgcatac
    37981 atacatggcc atggcggcaa gtgtgtctga aggagaaact cccgacgata aaaccaattt
    38041 acttcgtaag ggtgtgggca gatcagaatt tccggacatg ccgatatttc cggctatggt
    38101 tgtataagca taaatgcgag aagctgcagg agctgaaaac acatcgtgcc gtattaccga
    38161 agtaatgtcg acaatcgcct gatcgatcac agacatttgg gcttgcatgc attgggtgaa
    38221 caacaagcat attattatca ggtagcgcat ttcatggaga aaggtaagca atagtttgat
    38281 gtgataaaaa gctgctgcaa caattatacc tgaattgctg aaaacgtatc tcaataataa
    38341 taatcaatct taatactctt tatgacttcg aaagcatact ttaacaaccc gcaaaattga
    38401 aagaaattat ttttgaactg tacctaattt tcaaatccac ataccctaat gatgaaaaat
    38461 attttttttg cttgctgttt ttttctgatg aacagccata ttacacatgc acaaatcact
    38521 acggatataa ctccggttcc gggattaatt aattttcaaa atctgagaga ttactgcctg
    38581 aatgcaagtg gtgatgaaga ttttttcacc attcaaagtc caaatggtga aatatcacag
    38641 atagcaacga ggaaaaaaat caacggcaac tggagttcac ctgaattgat gcctttctgc
    38701 gatgctttta tgtatttgga gccatttctt actactaatg ggctgacttt gtattttgta
    38761 tccgaccgcc ctttaaacga tggcgacacc attaaaaaag attttgacat ctggatggtg
    38821 aagcgcgcgg caattacggc tgcctggtcg aaacccgtga atctgggcac tccggttaat
    38881 tcgacaaacg atgagtttta tccaagcctg accacctccg gcgatttata ttttacgatg
    38941 gcagcaccag aaggaatggg caaggatgat atttatatgt gtaaaatgac atcaacaggc
    39001 tatgaaaaac caacaatatt accatcgcca ataaacagtg cgggttacga attcaatgca
    39061 tttatttcac ccgacggcaa catcctgatt tatacgaagt ataatgc
//
LOCUS       DAOQHY010000007        36825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_451_length_36825_cov_5.340876, whole genome shotgun sequence.
ACCESSION   DAOQHY010000007 DAOQHY010000000
VERSION     DAOQHY010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 36825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36825
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_451_length_36825_cov_5.340876"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            617..2572
                     /gene="gyrB"
                     /locus_tag="PLL28_01240"
     CDS             617..2572
                     /gene="gyrB"
                     /locus_tag="PLL28_01240"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNF67964.1"
                     /translation="MEKEAEELKKNNGYSADSIQVLEGLEAVRKRPAMYIGDTGFKGL
                     HHLVYEVVDNSIDEALAGYCRNIEVSILPGNSIRVKDDGRGIPTELHKKENRSALEVV
                     MTVLHAGGKFDKDSYKVSGGLHGVGVSCVNALSTHLTAEVHREGKIFKQEYETGKPLY
                     AVKEVGESNITGTIITFHPDDTIFTVSEYRYDTLAARLRELAYLNKGIRLILTDERTA
                     DEAGNFVREEFYSEGGLIEFVRFLDGSRTPIIPEPIYAEGARDGVAVEIALQYNDSFN
                     ENVHSYVNNINTIEGGTHVAGFRRALTRTFKAYGDKEGMFEKAKVEISGDDFREGLTS
                     LISVKVPEPQFEGQTKTKLGNNEVLGIVDTISGEMLFNYLEENPKQARLIIGKVILAA
                     QARIAARKARELVQRKSAFGGGGLPGKLADCTDKDPQRGELYLVEGDSAGGTAKQGRD
                     RTFQAILPLRGKILNVEKALDWKIFDNEEIKNIFTALGVMPAEDKTLDTTKLRYHKII
                     IMTDADVDGSHITTLILTFFFRKCYELVQQGYIYIATPPLYLVKKGKDQRYCWNEEDR
                     KAAVADFSKGGSESSVHVQRYKGLGEMNAEQLWETTMNPETRTLRQVTIDSAAEADHI
                     FSMLMGEDVPPRREFIEANAKYANLDI"
     gene            complement(2646..3263)
                     /locus_tag="PLL28_01245"
     CDS             complement(2646..3263)
                     /locus_tag="PLL28_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67965.1"
                     /translation="MISFKRFLCSLFLICSLQIVQAQTQPIDPSDAETTNEESSNDDG
                     DSGDDKKNWVDNIVVGGNLGAQFGQATYIEASPIVGYRITEMLTAGVGFTYQYFSENY
                     NLATFADYKAQVLGPRVFVQHDLIYGLFAHAEFEHSWVKFTYEDPTYGEYTNDVSALF
                     LGMGYNYEVGSNSRFQIMLLYDVLHNIESIYYSPWVVRMGFNIGL"
     gene            3494..5623
                     /locus_tag="PLL28_01250"
     CDS             3494..5623
                     /locus_tag="PLL28_01250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF67966.1"
                     /translation="MKLLNKEQVDWRLILFVLLLVLLPFILFADSAAKSPLPEGATDP
                     AFNIESTPDTLKEAFPALYSSLKISLATIPTDTVYVIIHPGDQLNVAGGFGLNDTLRF
                     TPYPMALSQQEVKVKPWNDLILEGWHFGTLTFEIVTDAVEYAGLAIPDISYTIEDNEL
                     PPGIVQAVDMDTTLTEGLTGIDLLFSMATIPTDTVFLIIDPDDQLRITGIPGEAVTVE
                     YLPNASSLSFDGASIRAVDDAVYEGDHFGTVTFTIVTDDPVYSTFTIDPITWSIIDND
                     EVPSLILDFPPDTTLEEGLSGVDLLIRLSSIPTDTVFITFDPDAQLRVTGIADVPVTV
                     IYPPTAAALAFDGVSLRAYDDYIFEGDHSGTIQISIESNDPYYDAMSLPDMTFPIIDN
                     DNPPGLTYSIPDVLELSETGGELPIVVALQSVPTDTVKILVDPSLQLRITDPGVGVYM
                     IFPPNASALNDHILNIKAVDDADFEGTHTGSFSFNFETIDPDYALMDIPTQTVTIFDN
                     DLEPGIIFLDTFGLAGVEGDTSLDILIKLNSIPLNPVTIELTPDVQLDLGKGPGGLVK
                     YKFRSDSALIFQTVRIKIVDDHLIEGDHKGYVSVNITTEDPYYMIMSVPTFDVNITDN
                     DIVPVYNYNPLVFNAFPSVAGDLINIQSPENGKIDVIDANGKYMFGIENGIGSTSIDT
                     HSWPSGNYMVIWRSEENAYYARFIVLH"
     gene            5701..8115
                     /locus_tag="PLL28_01255"
     CDS             5701..8115
                     /locus_tag="PLL28_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HNF67967.1"
                     /translation="MQDHQHATSVLTCYHCGEQCKDTTFEIEDKHFCCFGCKTVYEIL
                     QDNDMCAYYDMTAHPGVQQRNKTDIRKYAVLDDQQVRANFVRFADGNQTHVTFYLPAM
                     HCSSCIWLLEHMHKLHPGIIQSRVDFPKKEVSVVFDERELALSKVAALLDSVGYEPLL
                     SMSDMDKKPEKTLQRSRLIKIGIAGFAFGNVMMLSFPEYLAGGEVTDAGLQSMFRFLS
                     LALALPVFFYCASEFYVSSWKSIKNKFLNIDVPIALAIIITFTRSLYAIFVEHGPGYF
                     DSMTGIVFFMLVGRYFQDLTYKTISFDRDYRSYFPLSVSLLKKGEEVEKPLSALETGD
                     LIVVRNNELIPADALLVKGKGQIDYSFVTGESEPIEKRIGELVYAGGRQTGAAIELKV
                     VKEVSQSYLTQLWNNIDVSRKEDERQSFVQMLGRYFALIVLIVSAIACGYWIMQGDYQ
                     RALDSLITPMIVACPCALLLSATFTNGNAIRYFGKYGIYLKNALVIEKMTKTDAIVFD
                     KTGTLTQSNTTSTEYIGEELSSVEKHMIAVVAAQSTHPYSKAIVRFLGNTMLYAVENF
                     KEFTGAGIEGMVDQVHIQIGSAKFVGVPNALQEEHGIAYIAIDNVVRGRFRFANKYRA
                     GLDDMISRLKQQYDVYILSGDNDADKEYLLQYFPAEHMFFNQSPHDKLKFIKQLQQRH
                     RKVMMLGDGLNDAGALQQSETGIAVAQSSNFTPASDVVMDVKQLINLPALFKYATAAR
                     RIIIGSFVLSIIYNVIGLYFALQGSLSPVVAAILMPLSTITIVSFTTGMTTLAAKWVM
                     GKNRAS"
     gene            8336..8515
                     /gene="ccoS"
                     /locus_tag="PLL28_01260"
     CDS             8336..8515
                     /gene="ccoS"
                     /locus_tag="PLL28_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HNF67968.1"
                     /translation="MSVILILIIASVLVAAGFLFAFIWSVKKGQFEDDITPGIRMLFD
                     DEKYIEKSHSTNKPK"
     gene            8522..10666
                     /gene="ccoN"
                     /locus_tag="PLL28_01265"
     CDS             8522..10666
                     /gene="ccoN"
                     /locus_tag="PLL28_01265"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HNF67969.1"
                     /translation="MDLVKVSYDNKIVKWFAIATVFWGIIGMTVGLWIALELVFPQLN
                     LGVPVLTFSRDRPIHTNAVIFAFVGNGIFMGVYYSLQRLCKTRMLSDFLSKLHFWGWQ
                     LIIVLAALSLSMGITTGKEYAELEWPIDILIALIWVVFGINMMGTIIKRRERHIYVGI
                     WFYIATFVTVAMLHIVNSLELPISLTKSYSIYAGVQDALVQWWYGHNAVAFFLTTPYL
                     GLMYYFLPKAANRPVYSYKLSIVHFWTLIFLYIWAGPHHLLYTALPHWAQTLGTVFSV
                     MLIAPSWGGMVNGLLTLRGAWDKVREDVVLKFMVVAVTAYGMSTFEGPMLSLKNVNAI
                     AHFTDWIVAHVHIGALGWNGFLTFGILYWLIPKMWRTTLYSKSLANLHFWIGTLGILI
                     YTVPMYWGGFTQALMWKDFTPDGHLTYSFLETSTQLKGMYLMRSIGGTLYLVGVFMMA
                     YNLVKTAKQGSFANNEDVEVPEMERVVKVPKNEFWHRALERRPVQFMVLALVMVIIGG
                     VVELVPTFLVKSNIPTISAVQPYTPLELQGRDIYVREGCYVCHSQMVRPFRSETERYG
                     EYSKAGEFVYDHPFQWGSKRTGPDLAREGVGWLKKSDSWHYMHLLDPRSLSAGSVMPE
                     YSWLHENELDITTTPAKIRAMQKLGVPYPEGYDQIANDDLMKQAEEIAASLKKDDIET
                     EPTVEVIAIIAYLQRLGKDLETENALNASNTK"
     gene            10676..10870
                     /locus_tag="PLL28_01270"
     CDS             10676..10870
                     /locus_tag="PLL28_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HNF67970.1"
                     /translation="MNFMAYLSDINGVSIYPIISLIIFFAFFIGLFVWVSRMSKSSVD
                     ECKQIPLEDNQPIHSTSNHQ"
     gene            10872..12032
                     /locus_tag="PLL28_01275"
     CDS             10872..12032
                     /locus_tag="PLL28_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HNF67971.1"
                     /translation="MKKLILNKHLMVALGAVLMTTPLMAQTTPAPEKNWYVHDGLFWL
                     LIAVAVILLYVIYALAEVVIWGAKKKISDRGNTTKILALVGTAVMLMSGNSLMAQAAS
                     APAAAPASGLLQSDYLPLYILIAIEVLAIAYMSIMLLQLSKKEQLEATGKAETWLSQL
                     WEKWNYKVPIEREDEMLISDHDYDGIHELDNGMPPWLQYMFFFTIGFAIVYLWYYNIG
                     NGPTQIDEYNASVEKAEIEKAAYLAAAGANYDENTVILSTDAAVLANGKSTFQQYCVP
                     CHGANAEGTTTAPNLTDDYWIHGGKINDLFKTVKYGVQGKAMASWQEVLDPKQIFEVT
                     NYIKSLHGSNPANAKAPEGALYVEGGEAAPADTSAVTPDSLTVQNPATTVVQ"
     gene            12113..13510
                     /gene="ccoG"
                     /locus_tag="PLL28_01280"
     CDS             12113..13510
                     /gene="ccoG"
                     /locus_tag="PLL28_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HNF67972.1"
                     /translation="MKDDKSFRDIIYTADEEGHRKWVFAAKPSGRFYNWRTIISYIYL
                     IAFFALPFIKVHGEPLFLFNIPDRKFILFGVIFWPQDFYLFALGMLTFLVFIIFFTVL
                     YGRLFCGWICPQTVFMEMVFRKIEYLFEGTAEHQRKLAKMQWNTEKILRKGGKQVVFF
                     LFSFLVGNTFLAYIIGVDRLYEIITEPLKENVGGLAIMMVFSFAFFIVFAFLREQICT
                     FACPYGRLQGVLLDKFSIVVAYDYKRGEPRGHHKHGEEDKQLGDCIDCGLCVRVCPTG
                     IDIRNGTQLECVNCTACIDACDSIMDKINKPRGLVRYSSENNIVTGEKLKFTPRMKAY
                     SLVLLLLVSVLTTLLATRKDVTATVTRSSGQLYQKRDSVLVTNIYNIKVANKTHESFP
                     IHLKLENVDGKVEMIGNEMHINSEDLAEGKFFIVLSRDQIHTMKMDVQIGVYNDDKKI
                     STVKTTFFGPVSKKK"
     gene            13513..13944
                     /locus_tag="PLL28_01285"
     CDS             13513..13944
                     /locus_tag="PLL28_01285"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNF67973.1"
                     /translation="MNWGRSILLVYVGFVALIMVMVFKSFNNGFDLVSEDYYADELKY
                     QDRINATENALPFADSIDVAVGNESIDIHFPASLAAMQSGEAYFYKPSDAKQDLRSPI
                     KMNDSGVQQFAKSEFSAGVYRLKMTWTMQDKTYYVEKLIKL"
     gene            13941..14687
                     /locus_tag="PLL28_01290"
     CDS             13941..14687
                     /locus_tag="PLL28_01290"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNF67974.1"
                     /translation="MTALLITAFITGLFGSLHCAGMCGPIALSFNMQGKGNAVINALL
                     YNASRIFAYALIGMLFGSLGVTFHYVGLQQVAAISMGVMILIIALITWLGAASSVKLP
                     LIKEYTNFIQRKIAQAFKSSSPSRIVALGFFNGLLPCGLVYLAVAGAIASGNTFVGAA
                     YMIIFGLGTLPLMFIFSAFGNWLGFRQRLFIRKYLPVFSITIAILLILRGLNLGIPYV
                     SPQFTHDQHNPVPADQHCSPAAQQEAHILQ"
     gene            14729..15823
                     /locus_tag="PLL28_01295"
     CDS             14729..15823
                     /locus_tag="PLL28_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNF67975.1"
                     /translation="MTVADITHYLETIAPLSLQESYDNAGLQTGHPLQDVNAILISLD
                     VTEAVVDEAIAQGCNMIVAHHPVVFTGLKRLTGKTYTERIIIKAVRNNIAIYAIHTNL
                     DNVETGVNAMIAEKLGLVHGRVLQPKPGLLRKLVTFAPEAHAGEVRQALFQAGAGHIG
                     NYDHCSFNVDGAGTFRADATANPFVGKRGVDHEEAETRIEVIYPQWAETAVLRALLKA
                     HPYEEVAYDLYALGNNHKGVGSGWIAELPQAMEESQFLAMVAGKMQAKGVRYTALLGR
                     DIRKVALCGGSGSFLIKDAIAAGADAFITADVKYHQFFDAEGRILLVDIGHFESEQYT
                     SDLLARAISEKFANFAVRLSNVNTNPINYL"
     gene            15827..16570
                     /locus_tag="PLL28_01300"
     CDS             15827..16570
                     /locus_tag="PLL28_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNF67976.1"
                     /translation="MAVAKEMSVKESMQALWKLQRIHSKIDDITTLKGELPMEVSDLE
                     DEIAGLETRMGKLENELKDIETEISNRKTTISNSKAHIKKYEQQQNAVKNSREFDALT
                     KEIELNKLDIELSEKKIREANFAKEAKSVTIEEAKVILDNKKKDLKKKKEELERIIGE
                     TEKEEAELAKKVEKAEKDLEPRLIQAYYRIRESYRNGLAVVSVARNSCGGCFSVVPPQ
                     RQAEIKQLKNIIICEHCGRILVDESVMEA"
     gene            16595..18073
                     /locus_tag="PLL28_01305"
     CDS             16595..18073
                     /locus_tag="PLL28_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67977.1"
                     /translation="MKQYIIVLFMAFFIPASAIAQKYDFNPRCVEAYTYIQELRLEKG
                     RALLDKEKKENPSNLIPYLIENYIDFLTTYINEDPDQFKSLEKNKSTRLDKLKTGDQT
                     SPYYLYCQAEVTIQWAFSKLKFEEYVGAFTDVRKAYLLLEENQKKFPDFIANNKSLGM
                     LHAIVGAIPDQYKWGINMLGMDGSIDQGLRELESILEYSETNTFVFKQEVYLYYAFIA
                     LYLGHDAQTAWDIVKDLDTKTNLINVFCKASVAMRTGRNDLALQTLQSRPSGSEYLPY
                     HYLDFMVGLAKARKLDSDGAQYFERFLKNYKGKNYIKEAYQKLGWLALIRGDKDKYWE
                     YQYYVKSRGDDLIDDDKQALYEAESGHVPNVTLLKSRLLFDGGYYKEALKLLEGKSVD
                     SFSDKKDKVEFTYRAGRIYDMAKQPDKAKGFYLATIKNGETLPYYYAAASAYYLGIIF
                     ETQKDYPNARKYYQKCVDSDNKEYQTSLDGLAKAGLNRVKGK"
     gene            complement(18143..19642)
                     /locus_tag="PLL28_01310"
     CDS             complement(18143..19642)
                     /locus_tag="PLL28_01310"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF67978.1"
                     /translation="MLKNQVRLAIILITGIISMVSTNAFSQNYRLVDKDREMTFARLS
                     VTGKDSLFYFIKTDSVALEGLDSVYYFSTQFQNVAGDPDCEWVRTDTSILGFKMLIKP
                     DAQATHVFFNQHGDSIFIKSVVNIGDYWTVYKWPDGSFIKATVVNKLERTILPDVEDT
                     ILRIQFTVFNALNVIMPDSFPTETKMDISKFHGLTEFFNFNIFPLPGDSIGRVLRGLT
                     NPYENIVDVDAYRAYNFSLGYEFHYRETSVPDNQSDADKRISAIKYFIQTKTPLPDGV
                     SYQVERVQYDTLYNDGVPSVNLKWDTIELTFNYADFAYLDAHEFTLFDHIHAGYSDWV
                     KDTSLYEGVPHKNVYDHYVYNDATYCLSNPDNISQPVEIVGDGLGQIYYLDSLSADAY
                     YELSMVYFQVGLITWGTPYDFSSLDVAINQLAVEHLYVYPNPTLNYLHIGIPVQDDTE
                     MFIFDIQGRLVQKPVMHQGLINVSNLSPGMYTLAVYSENSFKSATFIKE"
     gene            19749..20765
                     /gene="murB"
                     /locus_tag="PLL28_01315"
     CDS             19749..20765
                     /gene="murB"
                     /locus_tag="PLL28_01315"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNF67979.1"
                     /translation="MVQIQHNVSLKPFNTFGIEVQTSAFTSIKTVSDLRELIHAGTLQ
                     SSHMFLGGGSNVLFTKDFNGLVIKNECKGIYITKEDNRHIWITAGSGEVWHDLVMFTV
                     TRGLGGIENMSLIPGTVGAAPMQNIGAYGVELTNVFEQLTAIDLLTGEEVLFNHEMCA
                     FGYRESVFKHQLKGKYFIADVTLRLSKYPELQINYGAIKQVLAERGIVEPTIKDISDA
                     VIYIRQSKLPNPVVLGNAGSFFKNPEIAVEQYDELKQLYPTIPGYPTTPNKIKVPAGW
                     LIEQCGWKGKIVGHTGAHKDQALVLVNYGGASGNEILALSKEIQQSILAKFNIAISPE
                     VNII"
     gene            complement(20788..22266)
                     /locus_tag="PLL28_01320"
     CDS             complement(20788..22266)
                     /locus_tag="PLL28_01320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF67980.1"
                     /translation="MKTRLYTILLSIIVTTNSGKAQNWDLFPYGAKLSYLERSTTRFS
                     VLCNDSINFDGLNTIYFCNAKAPKSSWQHCYDLLNASIPYKGNKIYFTGNGNEYLIYN
                     NIDSTNGFYFNTDIGLGDFWAVPIVNPASLYDELTVTFDSIDYINVLGDFDSVRYYSI
                     GVSPEPEEGTIFEGNVFTLSKNHGLLSFPNFGRLVNGVFCPDCLLMKDLVGFIQTDDT
                     LGLTIPKWSDYIQLQPDDILVYSSSTIISSHGVNDVDYFVDKITSVDKYPDSIVVNFI
                     RNDIYPGRNLFSRSVVQALFEGYNSSYHYGAFEGNHPTLTDGLEDGFYYISSSDGYNI
                     NSTRFENATCIEYSLTQAYVDTVDCTRHAYSPTTYSISSGIGLISSYANIYGWVYTQT
                     LVGSILSGHASGEIFASQFGASNKAVIEIQLLPNPTSDVIILPIACNENESYQVYNQY
                     GSLVGRGPADNPVINISDLASGMYVVFVKHDNLNYAGKFLKL"
     gene            complement(22468..23358)
                     /locus_tag="PLL28_01325"
     CDS             complement(22468..23358)
                     /locus_tag="PLL28_01325"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNF67981.1"
                     /translation="MYNQPMLPAQTFTSKVIIVTGGGTGLGKSMTRYFLELGATVIIT
                     SRKIEVLSKTAEELSAATGSTCIPIVCDVRDYDAVEHMLQSAIEKCGKVDMLVNNAAG
                     NFISPTERLSHRAFDSVVDIVLKGTYNCTLACGKYWIENKLSASVLNIVTTYSWTGSG
                     YVVPSACGKAGVLALTRSLAVEWGKYHIRLNAIAPGPFPTEGAWSRLVPPGMEDDFDM
                     AKKNAVGRLGEHQELANLAAYLLSDFAAFMTGEVVVIDGAEWLKGAGEFNMLDKLPTQ
                     LWDMMELMRKERGERREERE"
     gene            23411..23809
                     /locus_tag="PLL28_01330"
     CDS             23411..23809
                     /locus_tag="PLL28_01330"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNF67982.1"
                     /translation="MATFSHQIKVRGFHCDMFQHVNNARYLEFLEETRWEWLNRMTSF
                     EFFEKHQMSFVVVSITIHYRWPSLLNDVLNITVETNHIGNRSASVKQVITRDGDSKLI
                     AEAEVTFAMIDNRTGKSVPISDELKAFFEN"
     gene            complement(23829..25118)
                     /locus_tag="PLL28_01335"
     CDS             complement(23829..25118)
                     /locus_tag="PLL28_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNF67983.1"
                     /translation="MGNGITSTQRTLKVSHMADNLIGSEIIKLAGEVNDRIKKGEKIY
                     NLTIGDFDPKIFPIPAELEKGIIAAYQNNETNYPAANGIDILRKAAQYFVEKHLGLSY
                     SPDEYLIAGGGRPLIYATYITLLDAGDKVIYPVPSWNNNHYTHLSNCVKVEVATRPEN
                     NFMPTAEEIAPHVQDATLIALCSPLNPTGTAFSKQALEEICDLVLAENARRPADAKPL
                     YILYDQLYWTLTFGDTEHFDPVSLRPELRDYVIYIDGISKAFSATGVRVGWSFGPKAI
                     IDKMRSILSHIGAWAPKAEQVASAHFLTNDAAVDSFLMQFKSQVQQRLDGFYHHFTAL
                     KNKGYAVDCISPQSAIYLTVRCDLRGKRKADGTVIQNMADVASFLLNEAKIALVPFYA
                     FGSEKESPWFRLSVGTCSLQDVDDSMASLEKALSQLQ"
     gene            complement(25262..26311)
                     /gene="selD"
                     /locus_tag="PLL28_01340"
     CDS             complement(25262..26311)
                     /gene="selD"
                     /locus_tag="PLL28_01340"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNF67984.1"
                     /translation="MSTSLPPIKLTQYSHGAGCGCKISPKILDEILQSVGKQQVFPGL
                     IVGNETKDDAAVYDLGNGTGLVSTTDFFMPIVDDPIKFGKIASVNAISDVYAMGGTPL
                     MAIAILGWPIDKVDPRYAGLVLDGARQACAEAGIPLAGGHSIDCPEPVFGLAVSGLVD
                     LKQLKTNAGASEGCSLFLTKKLGVGILTTAQKKDLLLPEHADIAPDSMMQLNKPGAAF
                     GKLEYIEAMTDVTGFGLLGHLTEMCEGSSVSAEIVFDKVPTIDRSILDHYLSQKSIPG
                     GTNRNFASYGHKIAPLTDTQKAILCDPQTSGGLLVAVTAGFESAFMSYAATLGLQLTS
                     FGKLVAPKEHLVYVS"
     gene            complement(26324..27370)
                     /gene="mnmH"
                     /locus_tag="PLL28_01345"
     CDS             complement(26324..27370)
                     /gene="mnmH"
                     /locus_tag="PLL28_01345"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HNF67985.1"
                     /translation="MTISAYLSDAGSLPLVDVRSPGEFATGHIPGAVNIPLFDNSERA
                     QVGILYKHEGRQAAIRKGLSITGPKLDKFIHEASALAKDNAIAVHCWRGGMRSQSMAT
                     LFDFAGFQTKVLKGGYKSYRGAVHALFSSQLTVYILGGRTGSGKTKILQALRNAGEQV
                     ADLEHLAHHKGSAFGDLGEEPQPSTEMFENLLFESIRNFDLSKPIWIEDESHLIGSVF
                     IPEALWQQMRNAPVFFCDVPIHERIQFLIHAYGQFPAEQLIGAVKKITKRLGGQHAKA
                     AVECFERGDLFKATEIVLGYYDKAYAYGLTQRAHQEIHNIEMSTINPEINAQQLIDHV
                     CRNAITEKNISTKS"
     gene            27418..28665
                     /locus_tag="PLL28_01350"
     CDS             27418..28665
                     /locus_tag="PLL28_01350"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNF67986.1"
                     /translation="MLNAIGKSFIALLLIASLHAQAQMPISTETYHATLDMQGQQLGF
                     QLELEITANSSEAYAPLNFTLVNGVEQIAGEVIKRNDSFVCIMPVFNTEIVFVGDNHL
                     QEIHGRWYDHSRQGNYFLPFKATQISQDEKRYRYFKQLTAPKANISGTFAVAFNDGES
                     TDNTVGIFEQKNNYLTGTFLTTTGDYRFLEGEVSGDKFYLSAFDGSHAFIFQGKIISN
                     DSIIGVFYSGIHYLSLFNGKRDANAKLPDESKLTFLKPGYDKISFSFPDENGKMVSLD
                     DAKFSNKAVIVTITGSWCPNCMDESSYLSEVEDKYKKQGLEIIALSFERKTDPETFAW
                     SINKVRTHFGTDFTYLNAGLPKTAGDALPMLNKVMGFPTMILLDRNHQVVAIHTGFSG
                     PATGKLFLDFQTKFEEELSRALK"
     gene            28825..30297
                     /locus_tag="PLL28_01355"
     CDS             28825..30297
                     /locus_tag="PLL28_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF67987.1"
                     /translation="MKYALYLMLLISTFSATAQVEITNISFEHNSADLSSDDLSKISE
                     IAGRLNASHDNVISVTMAGFTDTTGTVEYNEQLSWQRTKNVEKALHDLWPGEVTLEWY
                     GERNEGNAHAQTRSVDIIVSIETLPQVEIPKSNAENFIEVFSSVQQKAQQFYIPSNDS
                     SIIIGDQGTVIDIPANSFDIPTAYAGANVTITLKEYYLLSDMLLAGFTTQCGDDLLQS
                     GGMIDVNAYVAGKKVGLRDGKKLHIKFNTKRKLGEMELFAGERNEKGLVVWKRTKTPM
                     TATNTYEFISLLAPAYFKEVSDLKSCPYFFCGLRQQFGMNTTNSQNRRTYGKIDWPAY
                     RAYISEMKKSFGVKYMTELRRAIDEHNKKVGEINQYVVETSSLGLMNCDRFMNYPVEQ
                     RTKVIAESRQIDGASGFLAFTAYPAMLSVNYFEDGNIGFSNIGPDMNATLVLIRKDGE
                     KWMLSIQNIVTARDTRVTPAFVEVTPEELKTKLKMLDENG"
     gene            30497..31162
                     /locus_tag="PLL28_01360"
     CDS             30497..31162
                     /locus_tag="PLL28_01360"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNF67988.1"
                     /translation="MKHLFAITALFFTSYCYTQTSTTSYYGDVYLSTKAPQSKAKFSK
                     TTTLAADSTITTVVTDLKNNRIVSSESMKNNEAVGIWLKRHGGQTDTLDYQFDLIYAA
                     STCRESLPGLKNVWADNDSLHYKAPVLKDNESVYAAIGNEVYYPQFAKENAIEGKVFL
                     EFTITETGKIENIIVVRGVHISMDKEAVRTVKTLKFESLPMVNGVVTPLYVKMPLMFT
                     LAD"
     gene            31180..32040
                     /locus_tag="PLL28_01365"
     CDS             31180..32040
                     /locus_tag="PLL28_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67989.1"
                     /translation="MKTILLLLGVSIGATLFAQKNPTELAQPIVAEGKLLYKSEMASW
                     FGTDIFLEKFSDMNIVGGYFSYTEGDTSTCIFFSDAEKPEVLGEIQFDTTFNPSTAKV
                     DLERRSFTNLENNLFVIRSKALDELRSDTMFIRYNNTDFNLIPVIKDGERKVYVLTGT
                     ASNGVVLFGNDYLLTFDAENNLLTKKRIHQNLIPVYLNGPDAKNTVGTIHSHLPETGD
                     FMTATDICTAMLYQDITHWETITVVSEKYMNIWNCRTNTLTVVEKDIFKKIDEDQEKR
                     HGNKEKNNDD"
     gene            32151..32819
                     /locus_tag="PLL28_01370"
     CDS             32151..32819
                     /locus_tag="PLL28_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HNF67990.1"
                     /translation="MLKKLVVTKRIKKIFFRTFILGSVSVILLILFCNFRIEKAGKGK
                     LYSDVSAIPHQHVGLLLGTGKYMSDGSVNLFYKYRIEAALSLLKSNKIDYLVISGDNS
                     RPDYNEPAMMRDDLVAAGIDISRIYLDYAGFRTFDSVVRLREIFGTDSATIISQEFHN
                     ERALYIAGKENITAIGFNAQDVGGSGGFKTQLREYLARVKVFVDYIIHTKPHFLGPKV
                     QIPQ"
     gene            complement(32830..34242)
                     /locus_tag="PLL28_01375"
     CDS             complement(32830..34242)
                     /locus_tag="PLL28_01375"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNF67991.1"
                     /translation="MKHLLSAFLFVAIISLTNAQSIAPQGGGALPALPHDEISQEQYA
                     AMRSDVQHNIEVLSAQGKLVPAQQKTEALALEFPLAWNAGFSGYNFYGISNFADHNTA
                     FPGALLDWNCGARTYDTDAGYNHQGIDYFLWPFSWKMMQEEQVQIVAAAPGMIVARYD
                     GNFDQNCAFNPGSWNAVFVRHDDGSTAWYGHMKNGSVTAKGLGDFVETGEYLGLVGSS
                     GNSTGPHLHFELYDADGALMDPNSGACNEWNDESWWADQKPYIDPEINKLQTHFAPPT
                     FPPCPEVEITNESNTFNVGDVCYFALYAKDLSSTDLCHLKITRADGSVWYEWDFYQPA
                     DYFSASYWYWFYTIPEDVPEGVWTWSAELAGKYYEHQFIVGDMPVDVHENGSYTSVNA
                     YFQNNSLQLQLSSSIEQQSQIAIIDAMGRMVAEKSTILQMGDNLITIPCETLSSGIYF
                     IRILNSTTGATTTIPVCKQF"
     gene            34590..36434
                     /locus_tag="PLL28_01380"
     CDS             34590..36434
                     /locus_tag="PLL28_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67992.1"
                     /translation="MIALVEYSSEGSDYAAAKLLRIYKGALVVGNEIYISGFSNQYGP
                     HDMMHIGDQYIVFLNLMKPWGSANEYFEKAANDDPGIMKFADALFQNNAYYVWTPTAG
                     DYQVENGRVKFDLLNTGYHGNAALHSMKELDTFLAAYFEPAKRASFERKLIRKIKPAS
                     ASNDKTQALMMLYLLDYQAYNPIFEDYVHVKNEYSRFALTQVLGNIHNKASDAVLLLL
                     LDDRSSLVQGSSVRAMALCDPEIVGPALLSRLKDAGEYNLGPTTLMDPVRNSLSGGKY
                     QIIETLGDIGYTPAIPTLLGMLETRNEDDFEHIVDALRKLGTDEYAQYINLHLDSLHH
                     NMVYTLGQIIVRDSLSQCIPSLMYYISHHDRSFYPTEEKAVSYNAGLGFFKSDTVLNF
                     LSGDFVELMKTPYTGDVAYDTKLDWVKEYLLTFMHLGIDPHKDLVYDFMYEYYGFNSR
                     FRYEPVYFQKQQNIEDSITKLILEVLLPLEPNVVVSTRAFVDSNYNLLDYVSKFQIPK
                     PNNFVLQKINRLDTLTDAVSEKTTINNRHLIAEAANSSKSYGGARMKSVNSDLMMIFL
                     NYIAVFADEKDVSFIENLMKYYCANDTSTISMLNEYLEKARINASKKS"
     gene            36415..>36825
                     /locus_tag="PLL28_01385"
     CDS             36415..>36825
                     /locus_tag="PLL28_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67993.1"
                     /translation="MHQKKANKQPATIVTAADSNAAFPFELSNRINTNKITSWQTNGE
                     LKYSMRIPNFFGIEDLKVAIVQNITTDEDYGLPVRNTNLSFYNADSLLFTFDEEFLHD
                     EKISVLQDPMQMADINGDGLLDIKLIFRHWGSGRL"
BASE COUNT        11049 a         7279 c         7635 g        10862 t
ORIGIN      
        1 gtcgacagga aatgcgggcc tttctacttt ttcgcctttc tgggcaaatg cggccactga
       61 gcccatcagg catacgatag tgaggaattt cttcatgaaa gtgctatttt gcgacgaaga
      121 taatcccaaa aacaatacca gattttcacc aatttgaggt tatgcacagt ttttgcccct
      181 aatgatttaa aattttcatc aggatgtata cctttgtcct aaataactgc atcatgagtt
      241 ctcagttttt tgtaggtctt gttatcgttt gctacatcct tgttgttggc ggattgtttg
      301 tgatagaata tatcaaaggc aaacgcggtg gaaataccaa cggccactaa taatccatag
      361 ccctcctgtc cgatgcagag gataactcct cctgcctcct tttttgtacg atctgtaatc
      421 ttatttccaa gccggaaatg gccctctttt tgcaggcgat aaaccggtca ttttactacc
      481 ctcggtaatg tgcattaggc gtgaataaat tgcctttttt atgccttgat tttactaggt
      541 aatcaggggt aaaaatggta attttgcaga cgctcaaaac aggggtgttt ttactcctga
      601 aatgcaagtg aaaatcatgg aaaaagaagc agaagaatta aagaagaata acggttacag
      661 tgccgatagt attcaggtgc tcgaaggttt ggaagcggtg cggaaacgcc cggccatgta
      721 tatcggtgat accggtttca aaggactgca tcacctcgtt tatgaagtag tagacaactc
      781 tatcgatgaa gcccttgcag gttattgcag gaatatcgaa gtatctatcc ttccgggaaa
      841 tagtatccgt gtgaaagacg acggtcgggg tattcctaca gaattacata aaaaagaaaa
      901 tagatcggct ctcgaagtgg ttatgactgt gctgcatgcc ggtggtaaat tcgataagga
      961 ttcatacaag gtatcaggtg gtcttcacgg cgtgggtgtt agctgcgtga atgcattatc
     1021 tactcacctt accgccgaag tgcatcgcga aggcaaaata tttaagcagg aatacgaaac
     1081 gggtaagccg ctttatgccg taaaagaagt tggcgaatca aatatcacag gaactatcat
     1141 caccttccat cctgatgata ccatttttac ggtgtcagaa taccgatatg atactttagc
     1201 tgcgcgtttg cgtgaattgg catacttgaa caaaggaatt cgcctcatcc ttaccgatga
     1261 acgcacagcc gatgaggcag gtaatttcgt cagagaagaa ttttatagtg aaggtggatt
     1321 gattgagttc gttcgctttc ttgatggcag cagaacgcct atcattcccg aaccaattta
     1381 tgctgaaggt gcccgcgatg gcgttgcagt tgaaatcgcc ctgcaataca acgattcttt
     1441 caacgaaaat gtacattcat acgtcaacaa catcaacacc attgaaggtg gaacacatgt
     1501 tgccggtttc cgtcgcgcac ttaccagaac attcaaggct tatggcgata aggaaggaat
     1561 gttcgaaaaa gctaaggtgg aaatcagtgg tgatgacttc agagaaggcc ttacttcatt
     1621 gatttctgtg aaggtgcctg agcctcaatt cgaaggacag acaaaaacaa agcttggaaa
     1681 taatgaggtg ctcggaattg tggatactat ttcaggtgag atgctgttca actacctgga
     1741 agaaaatcct aaacaagcac gccttatcat cggtaaagta attcttgctg ctcaggcacg
     1801 tatcgctgcc cgcaaagcac gcgaacttgt gcagcgtaaa agtgctttcg gtggaggtgg
     1861 tttgccgggt aaactggccg attgcacaga taaagatcca caacgcggtg agttgtatct
     1921 tgttgagggt gactcggcag gtggtacagc caagcaaggt cgtgatcgta cttttcaagc
     1981 tatcctgcct ctgcgtggaa aaatattgaa tgttgaaaaa gcactcgact ggaaaatttt
     2041 tgataacgaa gagataaaaa atattttcac ggcactgggt gttatgccgg cagaagataa
     2101 aacgttggat actaccaaac tgcgctatca caaaatcatc atcatgacag atgccgatgt
     2161 cgatggtagc cacatcacca cacttattct gactttcttc tttagaaaat gttatgagtt
     2221 agtgcagcaa ggatatattt atattgctac tcctccgctg tatttagtga agaaaggaaa
     2281 agatcaacgc tattgttgga atgaagaaga tcgcaaagca gcagtggctg atttttctaa
     2341 aggcggaagc gaaagttcag tgcacgttca acgatacaaa ggtcttggtg aaatgaacgc
     2401 agagcaattg tgggaaacaa ctatgaaccc tgaaacccgc accctgcgcc aagttacaat
     2461 tgatagtgcc gctgaagcag atcatatttt cagtatgctg atgggtgaag atgtaccacc
     2521 acgccgcgaa ttcattgagg cgaatgctaa atacgcaaac ctggatattt aatgcatttc
     2581 taataaaaga aaagcgacat atctttcggt atgtcgcttt tttatttaat tatttttttg
     2641 tgattttaca atccaatatt gaagcccatt ctaacaaccc atggactata atatatcgat
     2701 tcgatattgt gcaatacatc gtataataac atgatttgga agcgagaatt tgatccaact
     2761 tcgtaattat atcccatgcc taagaataat gctgatacgt cattggtgta ttcgccatag
     2821 gttggatctt cataagtaaa tttgacccat gaatgctcga attctgcatg cgcaaataag
     2881 ccataaatta aatcgtgttg cacaaaaact ctcggtccca acacttgagc tttgtaatcg
     2941 gcaaaagttg caaggttgta attttcgcta aaatattgat aggtaaatcc aacaccggcg
     3001 gttagcattt ctgtaatcct gtatccgaca attggcgatg cctcaatgta ggtggcctgt
     3061 ccaaattgtg caccaagatt accgcctaca acaatgttgt ctacccaatt ctttttgtca
     3121 tcacctgaat ctccatcatc atttgaactt tcttcatttg ttgtttcggc atctgaaggg
     3181 tcaataggtt gcgtttgagc ctgaacaatt tgtaggctgc aaatcaggaa tagactgcac
     3241 aggaagcgtt taaacgatat catatgggtg gtaaaattag cgagaactca ttttaatgaa
     3301 gtacaaagcc ttgaaattta gggtggatcg ggaattttga gtccctaaac gcctatttca
     3361 ccgcaacaat tgttcctgaa atgttattcc gtacattaga taggtgattt caccccaaaa
     3421 aacaccaatt ttgctagaaa tcggttgtga acctatgtat acacctgtag ttttatcctc
     3481 agaaatacca tccatgaagc tgttaaacaa ggagcaggta gactggcgtc tgattctgtt
     3541 tgtgctatta ctcgtgctgc tcccctttat tttattcgca gactcggctg caaaatcacc
     3601 gctccctgag ggtgcaactg accctgcatt caacatcgag agcactcccg acaccttgaa
     3661 ggaagccttt ccagctcttt attccagcct gaaaatttct ttggctacta ttccaactga
     3721 tacggtttat gttattattc accccggcga tcaattaaat gtggcaggag gttttgggtt
     3781 gaatgacacc ttgcggttta ctccttaccc aatggccctt tcacaacaag aagtgaaggt
     3841 gaagccctgg aacgacctaa ttcttgaagg ttggcacttt gggacgctaa catttgaaat
     3901 tgttactgat gcagttgaat atgcgggctt ggcaattccg gacatcagtt atacgataga
     3961 ggataatgaa ctgccaccgg gaatcgtgca agcggtggat atggatacaa cactcacaga
     4021 gggattaaca ggcattgatt tgttgttctc catggcaact atacctacag atactgtttt
     4081 cctaattata gatccggacg atcaattacg cattaccgga atacccggtg aggcagtaac
     4141 cgtagaatat ctgccaaatg cttcaagctt gtcattcgat ggggcaagta ttcgtgctgt
     4201 tgatgatgct gtttatgaag gtgatcattt cggaaccgta acttttacaa ttgttacgga
     4261 tgatccggtt tattcgacat ttaccatcga tccaatcact tggagtatca tcgataacga
     4321 tgaagtcccg tcattaattc tggatttccc gcctgatact actcttgaag aaggattgtc
     4381 tggtgtagat ttgctcatca gattatcaag cattccgact gataccgttt ttataacctt
     4441 tgacccggat gcacaattgc gagttacagg cattgcagat gtacctgtga cagtcatata
     4501 tccaccaaca gctgctgcac tcgcatttga tggagtgtca ttaagggctt atgatgatta
     4561 tatttttgaa ggagaccata gcgggacaat tcagatatcc atagaatcaa atgatccgta
     4621 ctatgatgca atgtctttgc ctgatatgac atttccgatt atcgataatg acaatcctcc
     4681 cggattaaca tattctattc cggatgtgct tgaactttcg gaaaccggcg gtgaattacc
     4741 aatagtagtt gcattgcagt cggtaccgac agatactgta aaaatattgg ttgatccgag
     4801 tctgcagttg cgaataactg atcccggagt tggtgtttat atgattttcc ccccaaatgc
     4861 gagtgcactt aatgatcaca tcttaaatat taaggctgta gatgatgccg atttcgaagg
     4921 cacacataca ggctctttca gctttaattt tgaaactatt gatcccgatt atgcattaat
     4981 ggatattcct acgcaaacgg taaccatttt tgacaatgac cttgaaccgg gtattatctt
     5041 cctcgacacc tttggtttgg ccggggtaga aggtgatact tcattagata tcttgattaa
     5101 actaaacagt attccattaa atcccgtgac gattgaactt acaccggatg tgcaactcga
     5161 tctgggtaaa ggacctggtg gactggtgaa atataaattc cgcagcgact cggccttgat
     5221 ctttcagact gttcgtatta aaattgtgga tgaccacttg attgaaggtg atcataaggg
     5281 ttacgtttca gtaaatatta ctaccgaaga tccatactac atgatcatga gtgtgccaac
     5341 ctttgatgta aatattacag ataacgatat cgttccggtt tacaattaca acccacttgt
     5401 tttcaatgct tttccttcgg tcgctggtga cttgatcaat attcagtcgc ctgaaaatgg
     5461 caaaattgat gtcatcgatg ctaatggaaa gtatatgttc ggtattgaaa atggcatagg
     5521 ttctacctcg atagataccc atagctggcc ttcaggaaat tatatggtga tatggcgtag
     5581 tgaggaaaat gcctactatg caaggttcat agtgcttcat taagctgttt ttcaaatatg
     5641 gtgacattag tcatttttgc gcgatagcat caatcctagc tttggggcat attcaagccc
     5701 atgcaggatc atcaacatgc tacatctgtg cttacttgct accactgcgg tgagcaatgt
     5761 aaggatacta cttttgagat agaggataaa cacttctgtt gcttcggatg caaaactgtg
     5821 tatgagattt tgcaggataa cgatatgtgt gcatattacg atatgaccgc acatccgggc
     5881 gtgcagcaac gcaataaaac agatatccgt aaatacgctg tactcgatga tcaacaagtg
     5941 cgtgcaaatt ttgttcggtt tgctgatggt aaccaaacgc atgttacatt ttatctccct
     6001 gccatgcact gcagttcatg tatatggctg cttgaacaca tgcataaatt gcatcccgga
     6061 ataattcaat cacgtgtcga ttttcctaaa aaagaagttt ctgtcgtttt tgatgaacgt
     6121 gaattggcat tgagtaaagt cgccgcatta ctcgacagcg ttggttatga gccattgttg
     6181 agtatgagcg acatggataa aaaacctgaa aaaacattac agcgctctcg actaattaaa
     6241 attgggattg cggggtttgc atttggcaat gtgatgatgc tttcctttcc cgaatatctg
     6301 gcaggcggtg aagtgaccga tgcaggattg caaagtatgt tcagatttct ttcactcgca
     6361 ttggctctcc ctgtattttt ctattgcgcg agtgaattct atgtttcttc atggaagagc
     6421 attaaaaata aattcctcaa tatcgatgtg ccaattgcac tggcgataat tataaccttc
     6481 accagaagtc tttatgccat ttttgtagag catggtcccg gttatttcga ttccatgacc
     6541 ggaattgtat tctttatgtt ggttggaagg tattttcagg acctcactta caaaacaatt
     6601 tcctttgacc gcgattatcg cagttatttt cccctttccg tttcgctgtt gaaaaaaggt
     6661 gaagaggtag aaaagccact atcagcgctc gaaacaggtg atcttattgt tgtaagaaat
     6721 aatgaactga ttcctgccga tgccttactg gtgaaaggga aaggacaaat tgattacagc
     6781 tttgtaaccg gtgaatctga accaattgaa aaacgcattg gtgaattagt ttatgcaggc
     6841 ggacgacaga caggcgcagc tattgaatta aaagtggtga aggaagtatc gcaaagttat
     6901 ttgacccaat tgtggaataa tattgatgtt tcacgcaagg aagatgaacg tcaatcattt
     6961 gttcaaatgc tcggtagata ttttgcgttg attgtactca tcgtttctgc aattgcctgt
     7021 ggatactgga tcatgcaggg cgattatcaa cgtgcacttg attcattaat aacccccatg
     7081 attgtagcct gtccatgcgc attattgtta tcggcaacat tcaccaatgg caatgccata
     7141 cgctatttcg gtaaatacgg tatctatctt aaaaatgcac tggtaatcga aaaaatgacc
     7201 aagactgatg ccattgtttt tgataagaca ggcacactca ctcaatcaaa tacaacatca
     7261 acagaatata tcggcgagga gctttcaagt gtagaaaagc acatgatagc agtagtggca
     7321 gcgcaatcta cacatcctta cagtaaagct attgtgcgtt ttttgggaaa tacgatgttg
     7381 tatgcagtcg agaactttaa agagtttaca ggggctggaa ttgaaggaat ggttgatcag
     7441 gtgcatattc aaataggttc tgcaaaattt gtaggggttc ctaatgccct tcaggaagaa
     7501 catggtattg catacattgc cattgataat gtggtgagag gcagattccg ttttgcgaat
     7561 aaataccgtg caggtcttga tgacatgatt tcaagactaa aacagcagta tgatgtctat
     7621 attttaagtg gcgataatga tgcagacaag gaatatttgc ttcaatattt tcctgcagaa
     7681 catatgttct tcaaccagtc gcctcacgat aaacttaaat tcattaaaca attgcaacag
     7741 cgccatcgca aggtcatgat gttgggtgat ggcttgaatg atgccggagc attgcaacaa
     7801 agtgaaacag gtattgctgt tgctcaaagc agtaatttca cacctgccag cgatgtggta
     7861 atggatgtaa aacaattgat taatctgcct gcgttgttca aatatgctac tgctgctcga
     7921 cgtatcatta taggtagttt tgtgttaagc atcatttata atgtgattgg actgtacttt
     7981 gcattgcaag gatcactttc tcctgtagtc gcagcaatat taatgccatt aagtaccatc
     8041 accattgttt cattcacaac aggtatgacc acccttgcag ctaaatgggt aatgggtaaa
     8101 aatcgagctt cctgataagc aaaatcttca gtgattctgt gccggcaatt gacaatttat
     8161 tatttgtcta aactgtcatt ttttgcattc cgttttttgt caaaagactt gctgcctaag
     8221 cgtttcatca aatttatgga tttgacccct tcgtcaaaag atgataaaag tcaccttttg
     8281 tcgtgaagta tgtcatctgt gtcggatttg gttattatca acttagcgtc tgattatgag
     8341 tgtaattctg atattgataa tagcaagtgt actggtagct gcaggttttc tgtttgcatt
     8401 catatggtcg gttaagaaag ggcagtttga agatgatatc acccctggta tccgcatgct
     8461 tttcgacgac gaaaaatata tagagaaatc acattcaacc aataaaccta aatagttaat
     8521 tatggatctc gtaaaagtca gttatgacaa caagattgtg aaatggtttg cgatagcaac
     8581 tgttttttgg ggaattattg gcatgaccgt tggtttgtgg atcgcgttag aactcgtgtt
     8641 cccacaactc aatttaggtg tgccggtatt aaccttcagc cgcgaccgac caattcacac
     8701 gaacgccgtg atttttgcct tcgtaggtaa cggtatcttc atgggcgttt attattcatt
     8761 gcaacgcttg tgtaaaacac gtatgctcag cgattttctc agcaagcttc acttctgggg
     8821 atggcagttg atcatcgtgc ttgccgcact ttcactcagc atgggtatca ccaccggtaa
     8881 agaatatgcc gaacttgaat ggcctatcga tattttgata gcattgattt gggtggtatt
     8941 cggtatcaat atgatgggta ccatcatcaa gcgtcgcgag cgtcatattt atgtgggtat
     9001 ttggttttat atcgcaacat ttgttacggt tgccatgctg catattgtta actctttaga
     9061 gcttccaatt tcacttacaa aaagttattc tatatacgcc ggtgtgcaag atgcgcttgt
     9121 gcaatggtgg tatggtcaca atgcggttgc attcttcctt actacaccat atctgggttt
     9181 gatgtattac ttcttaccta aagcagctaa cagaccggtt tattcatata agctttccat
     9241 cgttcacttc tggacattga tattcttata tatctgggct ggtcctcacc acttattata
     9301 tactgcattg ccacactggg ctcaaacatt gggaaccgta ttctcagtaa tgttgattgc
     9361 gccatcatgg ggtggtatgg tgaacggatt gcttacgctc cgcggtgcat gggataaagt
     9421 acgtgaagat gtagtattga aattcatggt ggttgccgta actgcttatg gtatgtcaac
     9481 tttcgaaggc ccaatgcttt cattgaaaaa tgtaaatgct atcgcgcact tcaccgactg
     9541 gatcgtggca cacgtacaca ttggtgctct cggttggaac ggattcctca ccttcggtat
     9601 cctgtattgg ttgataccta aaatgtggag aacaacactt tactctaaat cattggcaaa
     9661 tctccatttc tggattggaa cattaggtat ccttatttat accgttccaa tgtattgggg
     9721 tggtttcaca caagccttga tgtggaaaga tttcacacct gatggacacc ttacatattc
     9781 attcctcgaa acaagtacac aattaaaagg catgtatctg atgcgttcaa ttggtggtac
     9841 cttgtattta gtaggtgtat tcatgatggc atataacctg gttaaaacag caaaacaagg
     9901 ttcatttgca aataatgaag atgttgaagt gcctgaaatg gaacgtgtag tgaaagtacc
     9961 taaaaatgaa ttctggcaca gagcactcga acgtcgccct gttcaattca tggtattggc
    10021 acttgtcatg gtaattattg gtggtgttgt agaattggtt cctacattcc tggtgaaaag
    10081 taatattcct accatcagtg cagttcaacc atatacaccg ctcgaattac aaggtcgcga
    10141 tatttatgtt agggaaggat gttatgtatg ccacagccaa atggttcgtc cgttccgttc
    10201 agaaactgag cgttatggtg aatattctaa agccggtgaa tttgtgtacg atcacccatt
    10261 ccagtgggga tcaaaacgca caggtccgga tttggctcgc gaaggtgtgg gctggttgaa
    10321 aaaatcagac tcatggcatt atatgcactt gctcgatcca cgatcattgt cggccggatc
    10381 agtgatgcct gaatattcat ggctgcatga aaatgagttg gatatcacaa ccactcctgc
    10441 taaaatcaga gccatgcaaa aactcggtgt tccatatcct gaaggttatg atcagatcgc
    10501 aaatgacgac ctgatgaaac aagcagaaga gatcgccgcc agtttgaaga aagatgatat
    10561 cgagactgaa cctactgttg aggttattgc catcattgca tacctccaaa gattaggtaa
    10621 agacctcgaa acagaaaatg cattaaatgc ttctaacaca aaataatctg ctgatatgaa
    10681 cttcatggct tatttatcag atatcaacgg cgtaagtata tacccgatta tatcgctgat
    10741 tattttcttc gctttcttca tcggactgtt tgtctgggta agcagaatga gtaaaagttc
    10801 tgtcgacgaa tgcaaacaga ttccgctgga agacaatcaa ccaattcact caacttcaaa
    10861 tcatcagtga aatgaaaaag ctgatcctaa ataaacacct gatggttgcc cttggcgctg
    10921 tgttaatgac aacaccactt atggcgcaaa ccactcctgc tcctgaaaag aactggtatg
    10981 ttcacgacgg actattttgg ctattgatcg cagtagcagt tatcctgtta tatgtgatct
    11041 atgcattggc tgaagtagtc atctggggcg caaaaaagaa aatctccgat cgcggtaata
    11101 ccaccaaaat attagcattg gtaggtactg ctgttatgtt aatgtcggga aattcactca
    11161 tggctcaagc tgcttctgct cctgctgcgg cacctgctag tggcctgttg caaagtgact
    11221 acctgccatt atacattctg atagctatcg aagtgcttgc aattgcatat atgagtatca
    11281 tgttgctgca actttcgaaa aaagaacaac tcgaagctac cggaaaagca gaaacttggt
    11341 tatcgcaatt gtgggagaaa tggaattaca aagtgccaat tgagcgcgaa gatgaaatgt
    11401 tgatctccga ccatgattat gatggaatcc atgaattgga taacggtatg ccaccatggt
    11461 tgcaatatat gttcttcttt accatcggct tcgcaattgt ttacttgtgg tattataaca
    11521 tcggcaacgg cccgacacaa attgatgaat acaatgcttc tgtagaaaaa gctgaaattg
    11581 aaaaagcagc ttacctcgca gcagcagggg ccaattatga tgaaaatact gtaatccttt
    11641 caacagatgc tgccgtattg gctaacggta aatctacctt tcagcaatat tgtgttcctt
    11701 gtcatggtgc taacgcggaa ggaactacca ctgcgccaaa cctaaccgac gactactgga
    11761 ttcacggcgg aaaaatcaac gatcttttta aaaccgtgaa atatggtgtt cagggtaaag
    11821 caatggcaag ttggcaggaa gtgctagatc ctaagcagat atttgaagta acaaactata
    11881 ttaaatcatt acatggttcc aatcctgcaa acgcaaaagc accggaaggt gcattgtatg
    11941 ttgaaggtgg agaagctgca ccggcagaca catcagcggt aactcctgat agtcttactg
    12001 tgcaaaatcc ggcaactact gtagtgcaat aaataacaat caaagtcggt ctgttcgcag
    12061 gctatgaata gaccgacgtt attttttttc aggcagaaga aagaaaagtg ttatgaaaga
    12121 cgataagtcg ttccgcgaca tcatctacac agcagatgaa gaaggccatc gcaaatgggt
    12181 cttcgcagca aaaccatcag gtaggtttta taactggaga acaattatca gttatattta
    12241 tctgattgca tttttcgccc tcccttttat caaggtacat ggcgaaccat tattcctttt
    12301 taatattccg gatcgcaaat tcatcctgtt cggcgtaatt ttttggcctc aggattttta
    12361 tttgtttgca ctcggtatgc tcaccttcct ggtattcatc attttcttta cagtactcta
    12421 cgggcgtttg ttctgcggat ggatttgtcc tcagacagtt ttcatggaaa tggtatttcg
    12481 caaaatcgaa tacctgtttg aaggaactgc cgaacatcaa cgcaaactgg caaaaatgca
    12541 atggaataca gaaaagatat tgaggaaagg cggtaaacaa gtggttttct tcctcttctc
    12601 atttttagta ggaaatactt ttctcgctta tataataggt gtagaccgac tctacgagat
    12661 cataaccgaa ccactgaagg aaaatgtggg tggactggct atcatgatgg tgttctcatt
    12721 tgcatttttt attgtttttg cattcttgcg cgagcagatt tgcaccttcg catgtcctta
    12781 tggtcgtttg cagggcgtgc tactggataa attctcgata gtagttgcat atgactataa
    12841 acgcggtgaa cctcgtggac atcataagca tggcgaagag gataaacaac tcggcgattg
    12901 tatcgactgc ggtttgtgtg tacgcgtatg tccaacagga attgatattc gcaacggaac
    12961 tcagctggaa tgtgtaaact gtactgcttg tatcgatgca tgtgattcta tcatggataa
    13021 aatcaacaag cctcgcggat tagtgcgata ttcttctgag aacaatattg taaccggtga
    13081 gaaattgaag ttcactccgc gcatgaaagc ctattcactg gtattgctat tattagtgag
    13141 tgtgcttaca actttattgg ctacaagaaa agatgtgaca gctacagtaa cgcgctcttc
    13201 aggacaatta tatcaaaaac gagatagcgt tttggttact aatatttata atattaaagt
    13261 ggcaaataaa acgcacgaaa gtttccctat ccatctgaaa ttggaaaatg ttgacggaaa
    13321 agtagaaatg atcgggaacg aaatgcatat taattcggaa gaccttgcag aaggtaaatt
    13381 ctttatagtg ttatcaagag atcagataca taccatgaaa atggacgttc agatcggtgt
    13441 ttataatgat gataagaaaa tcagtacagt aaaaacaact ttttttggac cggtttctaa
    13501 aaagaaataa ctatgaattg gggtagaagt attttactag tttatgttgg ctttgttgca
    13561 ctcattatgg tgatggtgtt caaaagcttt aacaatggtt ttgatttggt atcagaggat
    13621 tattatgccg atgagctaaa gtatcaggac cgtattaatg caacagaaaa tgccttgcca
    13681 tttgccgatt ctattgatgt ggccgtaggc aatgaatcca tcgatattca ctttccggca
    13741 tctctagcag ctatgcaaag cggggaagca tatttttaca aacccagcga tgcaaagcaa
    13801 gacctgcgtt ctccaattaa aatgaacgac tcaggtgttc agcaatttgc aaaaagtgaa
    13861 ttttccgcag gtgtctatcg tttgaaaatg acctggacca tgcaggataa aacctattac
    13921 gtagaaaagc tgattaaatt gtgacagcac ttctgatcac ggcatttatt accggtttat
    13981 ttggtagcct gcattgcgcc ggcatgtgcg gaccaattgc actgtcgttt aatatgcaag
    14041 gaaaaggaaa tgccgtgatc aacgcgctgt tatataatgc ttccagaatt tttgcttatg
    14101 cacttatcgg tatgttattt ggatcgctcg gcgttacttt tcactatgta ggattacaac
    14161 aagttgcagc tattagcatg ggagtaatga ttttgattat cgctttaatt acttggctgg
    14221 gagccgcttc atctgttaaa ttacccttga taaaagaata cacaaatttt atccaacgca
    14281 aaatcgcgca agcatttaaa tcatcaagtc cttctcgtat tgtggcatta ggttttttta
    14341 atgggctctt accatgcgga ctggtatatc tcgctgtagc aggcgctatt gcatcaggga
    14401 atacttttgt gggtgctgcg tatatgataa tatttggatt aggtacgctg cctttaatgt
    14461 tcattttctc cgccttcgga aactggctgg gattcaggca gcggttattt atcagaaaat
    14521 acctgcccgt attcagcatc acaattgcaa ttctcctcat cctgcgtggg cttaatctcg
    14581 gaattcctta tgtaagcccg caattcactc atgatcagca taatcctgtt cccgctgatc
    14641 aacattgtag tccagcagcg cagcaagagg cgcatattct tcaataagta taaatctttt
    14701 cctcccggca ttttatacct tcgcaggtat gactgtagcc gatattacac attatttgga
    14761 aacgatagca ccactatcgt tgcaggaatc atatgataac gccggattgc aaacagggca
    14821 tccgctgcag gatgtaaatg caatcctgat atcgctcgat gttactgagg cagtggtgga
    14881 tgaggccatt gcgcagggat gtaatatgat agtcgcccat catcctgtag tttttacggg
    14941 tttgaagagg cttaccggaa aaacgtatac cgaacgcatt attatcaaag ctgtgcgcaa
    15001 caatattgcc atctatgcca ttcacaccaa cctggacaat gttgagacgg gcgttaatgc
    15061 tatgatcgcc gaaaaattgg ggttggtgca cggtagggta ttacaaccca agcccggatt
    15121 gctccgaaaa ctagtgacat ttgctcctga agcgcatgca ggtgaggtgc gacaagcact
    15181 ttttcaagcc ggcgcggggc atatcgggaa ttatgatcat tgcagcttta atgtagatgg
    15241 cgccggcacc tttcgggctg atgctacggc aaatccattt gttggcaaaa gaggggtgga
    15301 ccatgaggaa gcggagacca ggatagaagt catttatccg cagtgggctg aaactgccgt
    15361 gcttcgtgcg cttttaaagg cccatcctta tgaggaagta gcctacgacc tttacgcact
    15421 tggcaacaat cataagggag tgggaagcgg atggattgca gaattgcccc aggctatgga
    15481 agaatctcaa tttttggcca tggtagccgg aaaaatgcag gcaaaaggag ttcgatatac
    15541 ggcgcttttg ggtcgcgata tcaggaaagt tgcgctttgt ggcggttccg gaagcttttt
    15601 gataaaggat gctattgcag ccggagctga tgctttcatc accgcagacg tcaagtacca
    15661 ccagtttttc gatgccgaag gacgcatttt gctggtagat atcggccatt ttgaaagcga
    15721 acaatatacc tcagacctgc tggcaagggc tattagcgaa aaatttgcta actttgcagt
    15781 ccgtttgtct aacgttaata ccaatccaat caactatcta taagctatgg ctgttgcgaa
    15841 agaaatgtca gtaaaagaaa gcatgcaagc actctggaaa ctccagagaa tacattctaa
    15901 gatcgatgat atcactaccc tgaagggcga attacccatg gaagtgagcg atctggaaga
    15961 tgaaattgca ggtcttgaaa caaggatggg aaaactggag aatgaattga aagatattga
    16021 aacagagatt tcaaatcgca aaacaaccat ctccaattct aaagctcaca ttaaaaaata
    16081 tgagcaacaa cagaatgcag taaaaaatag ccgcgagttt gatgcgctta caaaagagat
    16141 cgaattgaat aaactcgata tcgaattgag cgagaagaaa attcgtgaag caaattttgc
    16201 caaagaagcg aaatctgtta ccatcgaaga agcgaaagtg atccttgata ataagaagaa
    16261 agatcttaaa aagaaaaaag aggaactcga acgcatcatc ggtgaaactg aaaaagaaga
    16321 agctgaactc gctaaaaaag tagagaaagc agaaaaagac ctcgagccac gtttgatcca
    16381 ggcttattat cgcattcgtg aatcatatcg caacggactt gctgttgttt cagtagcacg
    16441 taactcatgc ggtggctgct tctcagttgt gcctccacag cgtcaggctg agatcaaaca
    16501 attaaaaaat atcattatct gcgaacactg cggacgcatc cttgttgatg agtctgtaat
    16561 ggaagcataa tataaattaa aacaccaaaa tgccatgaag caatatatca tcgtattgtt
    16621 catggcattt ttcattcccg caagcgccat cgcccagaaa tatgatttca accctcgttg
    16681 tgttgaagca tatacttaca tacaggagct tcgtctcgaa aagggtaggg cgcttctcga
    16741 caaagagaaa aaagaaaatc catccaatct cattccctac ctgattgaaa attacatcga
    16801 ttttttaact acttatataa atgaagaccc cgatcagttt aaatctcttg agaaaaataa
    16861 atctactcgc ctcgacaaac tgaaaaccgg cgatcaaacc tcaccctatt atctgtattg
    16921 tcaggctgaa gtaaccatcc aatgggcgtt ttccaaactt aaatttgaag aatatgtagg
    16981 ggcctttacc gatgtgcgaa aggcctattt attattagag gagaatcaga aaaaattccc
    17041 tgattttatt gctaataata aaagccttgg tatgcttcac gccatcgttg gtgccattcc
    17101 tgatcagtat aaatggggga taaatatgct cggtatggat ggttccattg accagggatt
    17161 gcgggaattg gaatcaatat tagaatactc agaaacgaat actttcgtgt ttaaacaaga
    17221 ggtttatctc tattacgcat ttatagcctt atacctcggt catgatgcac aaaccgcctg
    17281 ggatattgtg aaagacctcg acacaaaaac aaatctcatc aatgtatttt gtaaagccag
    17341 cgttgctatg cgaacaggaa gaaatgatct ggctttgcag accttacagt ccagaccctc
    17401 gggaagtgaa tatttgccat atcactacct cgatttcatg gtaggccttg ctaaagcgcg
    17461 taaattagat tccgatggtg cgcaatattt cgagcgattt ctgaaaaact ataaagggaa
    17521 aaattacatc aaagaagctt accaaaagtt aggatggctg gctttaatca gaggtgataa
    17581 agataaatat tgggaatatc aatactatgt aaaatcgcgc ggtgatgatc tcatcgatga
    17641 tgataagcaa gcattatatg aagcagaaag cggtcatgtg cccaatgtta ccctattgaa
    17701 atcgcgactg ttattcgatg gcggctatta taaagaggca ttaaaattat tggaaggaaa
    17761 gagcgtagat agcttttccg ataaaaaaga caaggttgaa tttacctatc gtgccggtcg
    17821 catttacgat atggcaaaac agcccgacaa agccaaaggt ttttatctgg ctaccattaa
    17881 aaatggcgag accttgccat actattatgc agcagcctct gcatattatc tgggtattat
    17941 cttcgaaaca caaaaagatt atcccaatgc ccgcaaatac taccaaaagt gtgtcgacag
    18001 cgacaacaaa gaataccaaa ccagtcttga cggcctggcc aaagccgggt tgaatagggt
    18061 gaaggggaag tgagtgttta tactgtgtat ctcctgttgc cctaatttaa tgaatttgaa
    18121 agtagtgttt gattcggaat tattattcct taataaatgt agcgcttttg aaactatttt
    18181 ctgaataaac tgctaaagta tacatacccg gactaaggtt gctcacattt atcaatcctt
    18241 gatgcatgac cggtttctgc accaatcgcc cttgaatatc aaaaataaac atttcagtat
    18301 catcctgaac cggaattcct atatgcaaat agttgagcgt tggatttggg taaacatata
    18361 aatgttcaac cgcaagctga ttaatcgcta cgtccaaact gctgaagtca taaggtgtcc
    18421 cccaagtaat taaacccact tgaaaataaa ccatgcttaa ttcataatat gcatcggctg
    18481 atagtgaatc aagataataa atttgcccca ggccatcacc gacaatttca accggttgac
    18541 tgatattatc agggtttgaa aggcaatatg tggcatcgtt gtaaacataa tgatcatata
    18601 catttttatg cgggacacct tcatagagac tggtgtcttt cacccaatct gagtaaccgg
    18661 catgtatatg atcgaaaagc gtaaattcgt gtgcatctaa atatgcaaaa tcggcataat
    18721 taaatgttaa ttcaattgtg tcccatttca agttgaccga tggaacgcca tcgttataaa
    18781 gggtgtcata ttgcacacgc tctacttgat agctgacacc atcgggtaga ggtgttttag
    18841 tctggataaa gtactttatt gcgcttatac gtttatcggc atcactttga ttatcaggga
    18901 ctgatgtttc tcgatagtga aattcatatc ccaaggaaaa attatatgct ctgtaagcat
    18961 ctacatcaac tatgttttca tacggattcg taagtccgcg aagcactcgt ccaatactat
    19021 ctccgggaag aggaaatata ttgaaattaa aaaattcagt gagcccatga aatttagaaa
    19081 tatccatttt tgtttctgta gggaaactat ctggcatgat gacatttaat gcattgaaaa
    19141 ctgtgaattg tattcggaga atggtatctt ccacatcggg taaaattgtt cgctccaatt
    19201 tatttacaac ggtagcttta atgaatgaac catcaggcca tttgtacaca gtccaatagt
    19261 cacctatgtt tacaaccgat ttaataaata tgctatcgcc atgttgatta aaaaagacat
    19321 gcgtagcttg tgcatcaggt ttgatcagca ttttaaatcc taagatgctt gtatcagtgc
    19381 gtacccattc gcaatccggg tcacctgcta cattttggaa ttgtgtactg aaataataaa
    19441 cagaatcaag cccttccaat gccacagaat ctgttttaat aaaatagaat agactatcct
    19501 tgccggttac tgaaagcctt gcaaaagtca tttccctgtc cttgtcaacc aatctgtaat
    19561 tctgactgaa agcattggtg cttaccatgg aaataatacc cgtaatcagt ataatcgcaa
    19621 gccgaacttg gtttttgagc atgttgaaaa gtttactgag tggcgatagc cgaataccta
    19681 aatttacgta aaaatactaa aagttacctg ttaaagggca tgccgggcag gctagcatac
    19741 ttgtaatgat ggttcaaatt caacacaacg tctctttaaa acccttcaat acctttggaa
    19801 tagaggtgca gacgtcggcg tttaccagta tcaaaacggt atctgacctt cgtgaactca
    19861 ttcatgcagg cactttgcaa tcaagccata tgtttttagg tggcggaagt aatgtgctgt
    19921 ttaccaaaga ctttaatgga cttgttatca agaatgaatg taaagggatc tatattacaa
    19981 aagaagacaa taggcatata tggattaccg caggaagtgg tgaggtatgg catgatctgg
    20041 taatgtttac cgtgacgcgt ggattgggtg gaatagaaaa tatgagtctt atccctggta
    20101 cagttggtgc agcgccaatg caaaatatag gcgcatacgg tgttgaactt actaatgtat
    20161 ttgaacaatt gacggctatc gatcttctaa caggtgagga agtattattc aatcacgaga
    20221 tgtgtgcttt cggatacaga gaatcggtat ttaagcatca attgaaagga aaatatttta
    20281 tagctgatgt caccttgcgt ctttccaagt atccggaact gcaaatcaat tatggcgcaa
    20341 ttaagcaggt tttggcagaa aggggaattg ttgaaccgac aataaaagac atttctgatg
    20401 cagtaattta tatccgtcaa agcaaattgc cgaaccccgt agtcttaggg aatgccggca
    20461 gcttctttaa gaatcctgaa attgcagttg aacaatatga tgaattgaaa caattgtatc
    20521 caacaatacc cggttatccc actactccta ataaaattaa agtacctgcc ggatggttga
    20581 ttgagcagtg tggttggaaa ggtaaaattg tcggacatac aggagctcac aaagatcaag
    20641 ccttggtttt agtgaattat ggaggcgcaa gtggtaatga aattttagca ctttccaagg
    20701 aaattcaaca atcaatactg gccaaattca atatcgcaat ctctccggaa gtaaatatta
    20761 tttgagttta aggtcagtat acgttcatca aagctttagg aatttacctg cataattcaa
    20821 gttatcatgt tttacaaaaa caacatacat acctgaagct agatcactaa tattaataac
    20881 aggattgtct gcagggccgc gacctacaag tgaaccatat tgattgtaaa cctgatatga
    20941 ctcattttca ttgcatgcta ttggcaggat aattacatca gatgttgggt ttgggagaag
    21001 ttgaatttca attacagctt tattcgatgc gccaaattga gatgcgaaaa tttcacctga
    21061 ggcgtgtcct gagagaattg aaccaacgag cgtttgagta taaacccatc cgtaaatatt
    21121 tgcataggaa gaaatgagtc ctataccact tgaaatagaa taagttgtag gtgaatatgc
    21181 gtgccgagta caatccacag tatcaacata agcttgtgtt aaagaatatt ctatgcatgt
    21241 tgcattttca aaccttgttg aattgatatt atacccgtca cttgaactaa tataatagaa
    21301 tccgtcttct aggccatcag ttagtgtagg gtgatttcct tcaaaagcgc cataatggta
    21361 tgaactgtta taaccctcaa ataatgcttg aaccacactt ctgctaaata gatttcgtcc
    21421 gggataaata tcatttctaa tgaaattaac tacaattgag tcaggatatt tgtcgacaga
    21481 tgtgatttta tctacgaagt aatctacatc atttacacca tggctggata tgattgtgct
    21541 tgagctatat actaatatgt catccggctg caattgaata taatccgacc atttcggaat
    21601 ggttaagccc agggtgtcat cagtttgaat aaatccaacc aagtctttca tcagcagaca
    21661 atcaggacag aacacgccat tcacgagcct gccaaaattc ggaaatgaaa gcagtccatg
    21721 gttctttgac aaggtaaaaa cattgccttc gaatatcgtt ccttcctcag gctccggaga
    21781 aactccaatt gaataatagc gaacgctgtc aaaatctcca agtacattaa tatagtcgat
    21841 tgaatcaaat gtaactgtca attcatcata taatgaagcg ggatttacga tggggacagc
    21901 ccaaaaatca cctaacccaa tgtcagtatt aaaataaaat ccgtttgtcg aatcaatgtt
    21961 attgtaaatc agatattcat ttccgttacc tgtgaaatat attttattgc ctttatatgg
    22021 aatgcttgca ttaagaagat cgtaacaatg ttgccatgat gatttaggag ctttagcatt
    22081 gcaaaaatag atagtattta atccgtcaaa gttgattgag tcgttacata ataccgagaa
    22141 tctggtggtt gatctctcca gatatgaaag tttagcgcca tatggaaata aatcccagtt
    22201 ttgagcttta ccactattag ttgtgacgat aattgatagt agtatggtat acaacctcgt
    22261 tttcatagac aaaataaaga taattgatta tccaaattta ttgtcacatc taggtaaata
    22321 ataccttaga ctgtctattc tcaatattta gcgccttagc ctcttcgcgt ctttgcgtga
    22381 cactagacac acctgcttac ttgctaaccg gcaaacttgc taactccggc aaaaatctca
    22441 atgagtatac catttcaatt tcacatccta ttctctttcc tctctcctct ctcctctctc
    22501 cttcctcatc agttccatca tatcccaaag ctgagttggt aacttatcaa gcatgttaaa
    22561 ctcgccggct cctttgagcc attctgcgcc atcgattaca acaacttcac ctgtcatgaa
    22621 agcagcaaaa tcagatagca gatatgctgc taaatttgcc aactcctgat gctctcccaa
    22681 tcgaccaacg gcattctttt ttgccatatc aaaatcatct tccatgccag gaggtaccaa
    22741 tctgctccat gcaccttcag taggaaatgg accgggagca attgcattta gacggatatg
    22801 atattttccc cattcaactg caagtgatct tgttagcgca agaacacccg ccttaccaca
    22861 tgcacttgga actacatagc cgcttcctgt ccatgaatat gttgtaacaa tatttaatac
    22921 acttgccgaa agtttatttt ctatccaata tttcccgcat gcaagtgtgc aattataggt
    22981 gccttttaaa acgatgtcaa caacagaatc aaaagcgcgg tgacttaatc gttctgttgg
    23041 tgaaatgaaa ttgcctgcag cgttatttac cagcatatct actttgccgc atttctcgat
    23101 cgctgattgt aacatgtgtt caactgcatc ataatcccga acatcacaaa ctataggaat
    23161 acatgtggat ccggtagctg ctgataattc ttctgctgtt tttgaaagta cttcaatctt
    23221 tcttgaagtg ataattactg ttgctcctaa ttcaaggaaa tacctagtca tgctttttcc
    23281 taatccggtt cctcctccgg ttacaataat tactttgctg gtaaaagttt gtgcaggtaa
    23341 catcggttga ttatacatat attgattttt gtataaaaat actcattctg ataatacata
    23401 cctttgtgcc atggcaacct tctcccacca aatcaaagtc cgcggcttcc attgcgatat
    23461 gtttcaacat gtgaataatg cccgatatct cgaatttctt gaggaaacac gctgggaatg
    23521 gctgaatcga atgacaagct tcgaattctt tgagaaacac caaatgagct ttgttgtggt
    23581 gagtatcact atccattatc gctggccgtc tttactcaac gatgtgttaa atattacggt
    23641 ggaaacgaat catattggca atcgcagcgc ttctgtaaaa caggtgatta ctcgcgatgg
    23701 cgatagcaag ctgatagcag aagcggaggt cacctttgcc atgatcgata accgtacagg
    23761 caagtcagtg cccatctcgg atgaactcaa agcttttttt gaaaattgat ggctacttcg
    23821 cgcagcaatt attgcagttg cgatagggct ttttctaaac tagccatgct atcatccaca
    23881 tcctgcaaac tacaagttcc tacacttaat cggaaccaag gactctcttt ttctgaacca
    23941 aatgcataaa acggaactaa ggcaatctta gcctcattca aaaggaagga tgcaacatct
    24001 gccatatttt gaatcaccgt tccatcagcc ttcctcttgc cgcggagatc acatcttacc
    24061 gtcagataaa tggcactttg cggagaaata caatctactg cataaccctt gtttttcaat
    24121 gccgtaaaat gatgataaaa tccatcaagt cgctgctgca cctgcgattt gaattgcatc
    24181 aaaaagctat caactgcagc atcattggta agaaagtgtg cactcgctac ctgctcagcc
    24241 tttggcgccc aagctccgat atgacttaga atactcctca ttttatcaat aatggctttc
    24301 ggcccgaaac tccatcctac acgcacgcct gttgccgaaa atgctttgct gataccgtcg
    24361 atataaatga cataatcacg caattccgga cgaagcgata caggatcgaa gtgctcagtg
    24421 tcgccaaaag ttaatgtcca atacaattgg tcgtataaaa tgtatagtgg tttcgcatct
    24481 gccggacgtc gggcattctc tgccaacact aaatcacaga tttcttcaag tgcttgtttc
    24541 gaaaatgctg ttcccgtcgg attcaaaggt gaacataaag caatcaaagt ggcatcttgc
    24601 acatgcggag caatttcttc cgctgtaggc atgaaattat tttccggcct cgtagcaact
    24661 tcaactttta cacaattact taaatgcgtg tagtgattat tattccaact aggtaccgga
    24721 taaattactt tgtcaccggc atccaataag gtaatatagg tagcgtaaat caatggtcgg
    24781 ccaccacctg caattaaata ttcatcgggt gaataggaga gaccaagatg tttttcgaca
    24841 aaatactgcg ccgcttttct taaaatatca ataccattgg cagctggata atttgtttca
    24901 ttgttttgat aggcagcaat aatacctttt tctaattctg caggtattgg aaatatttta
    24961 gggtcgaagt ctccaatggt aagattataa atcttttcac cttttttgat tctatcgttg
    25021 acctcgccgg caagcttaat gatctctgat ccgatcagat tatcggccat atgcgacacc
    25081 ttaagcgtgc gttgtgtgga tgttatacca ttgcccatag ctgcaaatta cgttaatttt
    25141 tttcgtaaaa ctatcatgcc aaaatagctt tgaaaatgac aatatatgcc actttccgcc
    25201 aaataaagcc tgtaaagcct tttagctgtg gagaagctgt gtattgaggc cgaaaacgct
    25261 ttcaggaaac gtataccaga tgctcttttg gtgctaccag cttgccaaaa gatgtcaatt
    25321 ggaggccaag tgtagctgca taagacataa atgccgactc gaatccggct gtaaccgcca
    25381 ccaataagcc tccgctggtt tgcggatcgc agagtatcgc tttttgcgtg tcggtcagcg
    25441 gtgcaatttt atgtccatag ctggcgaaat ttcggttggt gcctccagga attgattttt
    25501 gtgataaata atgatctaaa atgctcctgt caatggttgg taccttgtca aacacaattt
    25561 ctgcactcac actactgcct tcgcacattt cggtgagatg tcctagcaat ccgaatccgg
    25621 ttacgtcggt catagcctct atatattcca atttaccaaa tgccgctccc ggcttgttta
    25681 attgcatcat gctatccggt gcaatatcag catgctccgg tagtaaaaga tccttttttt
    25741 gagctgtggt taatataccg acgcctaatt tttttgtcag aaataaacta catccttccg
    25801 aagcacctgc gttcgttttt aattgcttta agtcaacaag cccactcacc gccaatccga
    25861 acacgggctc cggacaatca atactatgtc cgcctgcaag cggaatacct gcctctgcac
    25921 aagcctgacg cgcgccatcg agaacaagtc ctgcataacg cggatctacc ttgtcaatcg
    25981 gccatcccaa tattgcaatt gccataaggg gtgttcctcc catggcatag acatcgctga
    26041 tagcatttac actggcaatt ttgccgaatt taatagggtc gtcaacaatc ggcataaaaa
    26101 aatcggtagt gctaactagt cccgtcccgt tgccaagatc ataaacggca gcatcatcct
    26161 tagtttcatt gccaacaatc aaaccgggaa atacttgttg cttgcctaca gattgtaata
    26221 tttcatctaa tatttttgga gatattttac atccgcaacc ggcaccatgc gaatattgtg
    26281 ttaatttgat tggaggtaat gatgttgaca tatggtatta ttcttaagat tttgttgaaa
    26341 tatttttttc ggtgatggca ttcctgcaca catgatcaat taattgttgt gcattaattt
    26401 caggattaat tgttgacatt tcgatgttat gtatttcctg atgtgcacgt tgtgttaatc
    26461 cataggcata tgctttatca taataaccta aaacaatttc cgtagctttg aaaagatcac
    26521 ctcgctcaaa gcattcaacc gctgctttcg catgctgtcc gcccaatcgt ttggtgattt
    26581 tttttacggc accaattaat tgctcggcag gaaattgacc ataagcatga atgagaaatt
    26641 gtatgcgttc atgaattggc acatcgcaaa aaaatacagg tgcattgcgc atttgttgcc
    26701 atagtgcttc ggggataaat accgaaccta tcaaatgact ctcatcttct atccatatcg
    26761 gtttgctgag gtcgaagttt ctgatacttt cgaacagtaa attctcaaac atttcggtgc
    26821 tcggctgagg ctcttctcca agatcaccaa aggcacttcc tttgtgatgc gccaaatgtt
    26881 caagatctgc aacctgctct ccggcattgc gcagtgcttg caaaattttt gtttttcccg
    26941 aacccgtcct tccaccaagt atatatacag tcaactgaga tgaaaataaa gcatgtacag
    27001 ctccgcgata tgatttgtag cctcccttta acactttggt ctgaaaccct gcgaaatcaa
    27061 ataaggtagc catgctttga ctcctcatgc cgccacgcca acaatgtacc gctatcgcat
    27121 tatcttttgc caacgcactt gcctcatgaa taaacttgtc gagttttgga cccgtaatag
    27181 ataaaccctt tctgattgca gcttgtcttc cctcatgttt atataaaata cccacctggg
    27241 cacgttccga gttgtcaaat aagggaatgt tcaccgctcc cggaatatga cctgtggcaa
    27301 actctcccgg actgcgaaca tccaccaatg ggaggcttcc cgcatccgat aaatacgctg
    27361 atatggtcat ttccttaaac aaggtgtagc tttggtgtaa aattataaaa cttgaacatg
    27421 cttaatgcta taggtaaaag cttcattgcc ctgttgttga tcgcttcact gcatgcgcag
    27481 gctcaaatgc ccatcagtac agaaacatac catgcaactc tggacatgca ggggcagcaa
    27541 ctcggatttc aattagaact cgaaattacg gctaattcaa gtgaagcata tgccccgtta
    27601 aattttactt tagtaaatgg tgttgagcaa attgccggtg aagtgatcaa aaggaacgat
    27661 tcttttgtat gtatcatgcc ggtgtttaat acggaaattg tatttgttgg cgacaatcat
    27721 ttacaagaaa tccatggcag atggtatgat cattcaagac agggaaatta ttttcttccg
    27781 tttaaggcca cgcaaatttc acaggatgaa aagcggtaca gatatttcaa acaacttact
    27841 gccccgaaag caaatatcag cggaaccttt gctgtagcat ttaatgatgg cgaatctacc
    27901 gataacactg ttggtatttt cgaacagaaa aataattatc tgacaggcac atttctaacc
    27961 actaccggcg attatcgttt tctggaaggg gaggtgagtg gcgataagtt ttatctctct
    28021 gcattcgatg gctcacatgc ctttattttt caggggaaaa taatttccaa cgatagtatt
    28081 attggtgtat tctatagcgg aattcactat ctctccttat tcaatggtaa acgcgatgct
    28141 aatgccaaat tacctgatga aagcaaactc acctttttaa aacccggata tgataaaatc
    28201 agcttctctt tccctgatga aaatggtaaa atggtaagcc ttgatgatgc taaattttct
    28261 aacaaagctg ttattgtcac catcacaggt tcatggtgtc caaattgtat ggatgaatca
    28321 agttatttga gtgaggtgga agataaatat aagaagcagg gattagaaat tattgcactg
    28381 agtttcgagc gaaaaacaga tccggaaaca tttgcatgga gcatcaataa agtgcgcaca
    28441 cattttggaa cagatttcac atatctcaat gccggtttgc ccaaaactgc cggggatgca
    28501 ttgccaatgc tcaataaagt gatgggcttt cctaccatga tactgctcga tcgcaatcat
    28561 caggtagttg ccatacatac gggtttcagc ggaccggcta caggtaaact attcctcgat
    28621 tttcaaacga aatttgaaga agaactaagt agggcattga aatgaaacgt atagtgattg
    28681 aattaaatta atgcacttgt gtttaattcc gggtgcgacc atattggtta cctgcaatta
    28741 gccttgattc gatagcgata aattaaattt cccctatata tactaaagcg cagggcaact
    28801 tcgttatagc ccaaatccac gaaaatgaag tatgcacttt acctgatgct acttatttca
    28861 acgttttctg cgacagctca ggttgaaatc accaatatct cgttcgagca caatagtgct
    28921 gatttatcgt ctgatgacct ttctaaaatc agtgaaattg ccggtcggtt gaatgcatca
    28981 catgataatg tgatatctgt taccatggcc gggtttacgg atactacagg aacggttgaa
    29041 tataacgagc aactatcatg gcaacgcaca aaaaatgtag aaaaagcttt gcatgatttg
    29101 tggccggggg aagtaacact tgaatggtat ggcgagcgta acgaaggcaa tgcccatgca
    29161 caaacgcgtt cagtagatat tattgtaagc atagaaaccc ttcctcaagt tgaaattccc
    29221 aaatcaaacg ccgaaaattt tattgaagta ttttcatcag tgcaacagaa ggcacaacag
    29281 ttttatatac caagtaatga ctcctccata attattggtg atcagggaac agtaattgat
    29341 attcctgcaa atagttttga tatccctacc gcatatgccg gtgctaatgt aaccataaca
    29401 ctaaaagaat attatttact cagcgatatg ctgctggcag gttttactac gcaatgtggc
    29461 gatgatttat tgcagtcggg tggtatgata gatgttaatg cctatgttgc cggaaaaaag
    29521 gttgggctga gggatggaaa gaagttacac atcaaattta ataccaagcg taaacttggc
    29581 gaaatggaat tatttgccgg tgaacgaaac gaaaaaggtt tggttgtctg gaagcggact
    29641 aaaacaccta tgactgccac taacacctat gaatttataa gcctcctcgc acctgcttat
    29701 tttaaggaag tttcagacct aaaaagttgt ccctactttt tctgtggttt gcgacagcaa
    29761 tttggaatga atactacgaa ttcgcaaaat cgccggactt atggaaaaat tgattggccg
    29821 gcatacagag catatatcag cgaaatgaaa aagtcatttg gcgtgaaata tatgactgaa
    29881 ttaaggaggg caatagacga acacaataag aaagttggtg aaataaacca atatgttgta
    29941 gaaacttctt ccctcggttt gatgaattgt gataggttta tgaattatcc tgtcgaacaa
    30001 cgaacaaagg taattgccga atccaggcag attgatggcg caagtggctt tcttgccttc
    30061 acagcctatc ctgccatgtt atctgttaat tatttcgaag acggaaatat tggattctca
    30121 aatatcggtc ctgatatgaa tgccacctta gtgctcatca ggaaagatgg tgaaaagtgg
    30181 atgctatcca tccaaaatat tgtcactgct cgcgacaccc gggtcacacc cgccttcgtt
    30241 gaggttaccc cggaagaact aaagaccaaa cttaaaatgt tggatgaaaa tggataaaac
    30301 ttaagcattc cggcacctat tcttcgcgcc ttcgctactt accgtctttg cgcgaaactt
    30361 tgttggaggt ttgttagact gaaattatca gattgctatc ttcttaattc atttgattta
    30421 ggtagcaatc tgtttattta tgtatagtct tgatgcattg atactctttc cctatcttta
    30481 acaggtaaac tccaatatga agcacctttt tgctataact gcgctcttct ttacctcata
    30541 ttgttataca caaacttcta ctacttccta ttacggtgat gtgtatttgt caacgaaagc
    30601 accacaatca aaggctaaat tttcaaaaac tactacgcta gcagcagata gtactatcac
    30661 aacggtggta actgatttaa aaaacaaccg gatcgtgagc agcgaatcca tgaaaaacaa
    30721 tgaagctgtc ggcatttggt tgaagcgcca tggcggacaa actgataccc tggattatca
    30781 attcgatctg atatatgcgg catcaacttg cagggaaagc ttgcccggac taaaaaatgt
    30841 ttgggctgat aatgacagcc ttcactataa agcgccggtg ctcaaggata atgaaagcgt
    30901 atatgcagca atcggtaatg aagtgtatta tccgcaattt gccaaagaaa atgccattga
    30961 aggaaaagta tttcttgaat tcacaattac agaaaccgga aaaattgaaa atattatcgt
    31021 ggttagaggc gtacatattt ctatggataa ggaagcagtg agaacggtta aaacattaaa
    31081 attcgaatca ttgccaatgg taaacggtgt tgttacacca ctctatgtta aaatgccttt
    31141 aatgtttacc ttggccgatt aaaattaaat ttgaatcaca tgaaaactat attgttattg
    31201 cttggcgttt ccatcggtgc aacgcttttc gcacagaaaa acccaacaga acttgcccaa
    31261 cccatagtag ccgaaggtaa gttgttgtac aaatcggaaa tggcatcatg gtttggcacc
    31321 gatattttct tggagaaatt ctctgacatg aatattgtgg gaggctattt ttcttatact
    31381 gaaggcgata cctctacatg tattttcttc tccgatgcag aaaaacctga agtgcttggg
    31441 gaaattcagt ttgacactac ctttaatccc tcaaccgcta aagttgattt ggagagacgc
    31501 agcttcacaa accttgaaaa taatcttttc gtgatccgaa gtaaagcgct tgatgaattg
    31561 cgttcggata ctatgttcat cagatataac aatacagatt ttaatctgat accggtaatt
    31621 aaggatggcg aaaggaaagt atatgtgctg acaggaactg caagtaatgg ggtggtatta
    31681 tttggtaatg actacttact cacttttgat gctgaaaata acttattgac taagaagcga
    31741 atacatcaaa accttattcc tgtatatctc aatggtcccg atgcaaagaa tacagtgggc
    31801 actatacaca gccatcttcc ggaaacgggc gactttatga ccgccacaga tatctgcaca
    31861 gccatgctat atcaagatat tacccattgg gaaacaatta cggttgtgtc tgaaaagtat
    31921 atgaatatct ggaattgccg gacaaatacc ctgactgtag tcgaaaaaga tatcttcaaa
    31981 aaaatcgatg aagatcagga aaaacgccat ggcaataagg aaaagaataa tgatgattaa
    32041 gccgcattca aagtgcaaat gaatgccgaa gaaacttgtt acaaattatg ccattatcat
    32101 ttttctatat ggttttcaag ataatggctt aagataagaa aaaaaatgat atgcttaaaa
    32161 aattagttgt gacaaaacgt attaaaaaaa tatttttccg aacttttatt ttaggttctg
    32221 tttccgttat tctgcttata cttttttgta attttcgtat cgaaaaagcc ggcaagggaa
    32281 agttgtatag cgatgtaagt gccatcccac atcagcatgt cggcctgctg cttggaacgg
    32341 ggaagtatat gagcgacggt tccgtaaatc tgttctacaa atatcgtatt gaagccgcac
    32401 tgtcgctttt gaaaagtaat aaaatcgatt accttgtaat cagcggtgat aatagtcgac
    32461 ccgattacaa cgaacctgcc atgatgcgcg atgatttagt tgctgcaggt attgatatta
    32521 gccgtattta cctcgattac gctggcttcc ggactttcga ttctgttgtg cgattgcgtg
    32581 aaatattcgg aacagattca gctacaatca tttcacagga atttcataat gaaagagctt
    32641 tatatatagc cggtaaagaa aatattacgg caataggatt caatgcgcaa gatgtgggcg
    32701 gaagtggtgg ttttaaaaca caacttcgtg aatatctggc aagagtaaaa gtttttgtag
    32761 attacatcat ccacacaaaa ccacatttcc tggggccaaa agtgcaaata cctcaatgag
    32821 agccttgaat taaaattgct tacaaaccgg tattgtagtt gtggctccgg tagtggaatt
    32881 caatattcgg ataaaataaa taccgcttga caatgtttca catggtattg tgattaaatt
    32941 gtcacccatc tgcaaaatgg tagatttttc tgcaaccatc cttcccattg cgtctataat
    33001 tgcaatttga gattgttgtt cgatggagct actcaattgc aattgtaagc tgttattttg
    33061 aaaataggca tttacactag tgtaagaacc attttcatgc acgtcaaccg gcatatcacc
    33121 tacaataaat tgatgctcat aatatttccc tgccaactct gcgctccatg tccacacgcc
    33181 ttccggaaca tcttccggga tggtgtaaaa ccaataccag taagatgcgc tgaaataatc
    33241 tgcgggctga taaaagtccc actcatacca cacactgccg tctgcacgtg taattttcaa
    33301 atggcaaagg tctgtcgagg ataagtcctt agcatacaat gcaaaataac aaacatcgcc
    33361 aacattaaat gtattcgatt cattggtgat ttcaacttcc ggacaaggtg gaaatgttgg
    33421 tggtgcaaaa tgtgtttgaa gtttattgat ttccggatcg atatatggtt tttgatctgc
    33481 ccaccagctc tcatcattcc attcgttaca agcgccggag ttggggtcca ttaatgcacc
    33541 atctgcatcg tataattcga aatgcaaatg tggacctgta gaattgcctg agctgcccac
    33601 aagtcctaaa tattctcctg tttcaacaaa atcgccaagc ccctttgccg taacagaccc
    33661 gtttttcata tgaccatacc atgccgtaga gccatcatca tgacgaacaa aaactgcatt
    33721 ccatgagccc ggattaaatg cgcaattctg atcgaaattg ccgtcgtatc ttgctacaat
    33781 cattcccgga gctgcagcaa caatttgtac ttgttcctct tgcatcattt tccatgaaaa
    33841 cggccataag aaataatcta tgccctgatg gttgtagcct gcatcggtgt catacgttct
    33901 tgcaccacaa ttccaatcca gtaaggcacc cggaaacgca gtattgtgat ccgcaaaatt
    33961 tgaaatccca tagaaattat atccactgaa cccggcattc catgcaagcg gaaattctaa
    34021 agcaagtgcc tcggtttttt gttgtgcagg gactagtttg ccttgtgcac ttaaaacttc
    34081 aatattgtgt tgcacgtccg aacgcatagc agcatattgc tcctgactta tttcatcatg
    34141 gggcaaagca ggtaatgctc caccgccttg aggtgcaata gattgcgcat ttgtgagtga
    34201 gattattgca acgaaaagga atgcacttag gaggtgtttc atgatatatt ttaagtaaaa
    34261 ttaataattg tgattgaata cttcaaaaaa tggcattgct gtgtatgtaa taattgattt
    34321 atttcgttac aataaattca ccaaatgaaa ggctgagcca tcgattgtgc tctattgctt
    34381 tggagtaatt ataatagtcc ggaaaaacaa taaatgttaa aatttattgt taatttcaag
    34441 ttcgatgcaa ataataatgt ttaaaaatgt atttactgaa aagaaattgg tgtttcctgt
    34501 tgggcatgtg gttggtgacc tgtttcaacc atgccaaggc tgatacttgg tgggatcctt
    34561 cagcaaagga gatgctggat agttccgatg tgatcgcatt ggttgaatat tcttcggaag
    34621 gaagtgatta tgctgctgcc aagttgttgc gaatatataa aggagctttg gttgtaggaa
    34681 atgagatata tatctctgga tttagcaatc aatatggccc acatgatatg atgcatatcg
    34741 gcgaccaata catcgtattt ttgaatttga tgaagccttg gggctcggca aatgaatatt
    34801 ttgaaaaagc agctaatgat gatccgggga ttatgaaatt tgctgatgcc ctttttcaaa
    34861 ataacgccta ctatgtttgg actccaactg cgggagatta ccaggtcgaa aacggtagag
    34921 tcaagtttga tttacttaat acgggctatc atggaaatgc agctttgcat tcaatgaaag
    34981 aattagatac gtttttagca gcatattttg aaccggcaaa gcgagcaagt tttgagcgta
    35041 aactaattcg aaaaatcaaa ccggcttctg ccagtaacga taaaacgcaa gcactcatga
    35101 tgttgtattt attggattat caagcctata atccaatttt tgaagattac gtccatgtga
    35161 aaaatgagta ttcgcgattc gccctgacac aagttttagg aaatattcat aataaagcgt
    35221 ctgacgctgt tttgttgttg ctacttgacg ataggagcag cttggtgcaa ggttcgtcag
    35281 ttcgtgcaat ggcattatgt gatcctgaaa ttgttggacc tgctttattg tcgaggctta
    35341 aggatgccgg cgaatacaac cttggtccga ctaccttaat ggatcccgta aggaattcac
    35401 ttagtggtgg caaatatcag attattgaaa cactgggaga tatcggttat acccctgcga
    35461 ttcctacact gctaggtatg ctggaaacaa gaaatgaaga tgactttgag catatcgtag
    35521 atgctttgcg aaaactaggt acagatgagt atgcgcaata tattaatctg catttagata
    35581 gtttacatca taatatggtt tatacacttg ggcagatcat agtaagagat agcctgtccc
    35641 agtgtatccc atcattaatg tattacatat cgcaccatga taggagcttt tatcccaccg
    35701 aagagaaagc tgtatcctat aatgcagggt taggcttttt taaatcagat acagtgttaa
    35761 attttttgtc cggtgatttc gttgagttga tgaaaacacc ttacacagga gatgttgcat
    35821 atgacactaa gctcgactgg gtcaaagagt accttttgac ctttatgcat ttaggaatag
    35881 acccacataa ggatcttgta tatgacttca tgtacgaata ttatggattt aattccagat
    35941 ttcgatatga accggtttat tttcaaaagc aacaaaacat tgaggattca ataactaaac
    36001 ttatattgga agtgctgttg ccattagaac ctaatgtggt tgtgtcaact agagcatttg
    36061 ttgacagtaa ttacaatctt ttggattacg taagcaagtt tcaaattccc aaaccaaata
    36121 attttgtact tcaaaaaatc aataggctgg ataccttgac cgacgcagtg agcgaaaaaa
    36181 caactatcaa taatcgccat ttaatagctg aagcggctaa ttctagtaag agctatggag
    36241 gagcaaggat gaaatcagta aattcagact taatgatgat cttcctgaat tatattgcag
    36301 tgtttgccga cgaaaaggat gtttcattta ttgagaattt gatgaagtat tattgcgcta
    36361 atgatacatc tacaattagc atgctaaacg aatatttaga gaaggcacgt attaatgcat
    36421 caaaaaaaag ctaataaaca gcctgctaca attgtgactg cagccgatag taatgctgct
    36481 tttccttttg agctatcgaa ccgaattaat actaataaga taactagttg gcagacaaac
    36541 ggagagctga aatattctat gaggattccg aacttttttg gaattgaaga cttaaaggtg
    36601 gcaattgtac aaaacattac aacagacgaa gattatggat tgccggtaag aaatacaaac
    36661 ttgtcatttt ataatgctga ttctttgctt tttacttttg atgaagagtt tttacatgat
    36721 gaaaaaattt cggtgcttca ggatcccatg caaatggctg atatcaatgg cgatggtttg
    36781 ctcgatataa aactaatatt caggcattgg ggctcgggta gatta
//
LOCUS       DAOQHY010000008        36785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_452_length_36785_cov_5.930411, whole genome shotgun sequence.
ACCESSION   DAOQHY010000008 DAOQHY010000000
VERSION     DAOQHY010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 36785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36785
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_452_length_36785_cov_5.930411"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1600)
                     /gene="dnaE"
                     /locus_tag="PLL28_01390"
     CDS             complement(<1..1600)
                     /gene="dnaE"
                     /locus_tag="PLL28_01390"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNF67994.1"
                     /translation="MSTFSHLHNHTMFSLLDGASDIGRLYKKAAADGMPALAITDHGN
                     MFGAFQFVAEAYKHKGEDGKVKVKPIVGCEFYLVADRTRKQFSREEKDIRYHQVLLAK
                     NETGYKNLVKMCSLGYMEGMYGKYPRIDKTLIEKYHEGLIATTCCLAAEVPRTILKKG
                     REEGEKVFKWWLDIFGEDYYIELQRHDIPEQETLNNILLELADKYNVKVIATNDAHYV
                     DQQDANAHDILLCINTGEKQATPKMKDFGDDDMIIKGKRFAFYNDQFYFKTQSEMNAL
                     FHDVPQALDNTNEIVDKVELLDLKRDILLPAFPIPDAFKIHGDDTTNQWEYLKHLTME
                     GAHKRYGTMDAETEERINFELFTIKTMGFAGYFLIVMDFIRAGRELGVFVGPGRGSAA
                     GSVVAYCIGITNIDPIKYNLLFERFLNPDRKSMPDIDTDFDDDGRQKVIDYVVDKYGK
                     NQVAQLVTYGTMAAKTSIKDVARVMDLPIADSNALSKLVPERPGISLKRLIQAPISGD
                     GSLSEKENLTPDELNNAKALRAIAE"
     gene            complement(1691..3355)
                     /locus_tag="PLL28_01395"
     CDS             complement(1691..3355)
                     /locus_tag="PLL28_01395"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNF67995.1"
                     /translation="MNNIFSNIVHWLSYDPENPLIFTRLFFWAFFAVVLLGYSFVYKK
                     VNVRTIYLLAASFFFYYQTSGVFLILLIFTIISDYNWGRLLAKTETPWKRRMFLTFSV
                     VLNLTLLCYFKYAFFFTENLNEVAGTDITFFNHFAHFTNTWFGTSFSVDKLILPIGIS
                     FFTFQSLSYTIDMYRRKLEPVKNIFDYGFFVCFFPHLVAGPIVKAHDFIYQIYQPYNL
                     SKREYGMAFFWILNGLGKKIMADYVGVNFVDRIFSHPELYSGVEVVLGIFGYSLQIYA
                     DFSGYTDMAIGIALLLGFRLKTNFRSPYKAESTSEFWKRWHISLSSWLKEYLYIPLGG
                     NRNGTVGSYVAISILGIFMVLLYGNVTLIYILLAIALVLILIARIFPKFRHSVNTNIN
                     ILVTMLLGGLWHGSSWMFIIWGGLNGLGLMLHKGWERINPIKNTSGVGYRAFAIFLTL
                     VFISFTRIWFRSDSLDTVGIIFDRISHHFGGELIENVLWGYRTVLSVIVVGYLIHWIP
                     ESTKQQYREWFATRSEPALVAILIGYIFLLYQAVSADMQPFIYFQF"
     gene            complement(3362..4570)
                     /locus_tag="PLL28_01400"
     CDS             complement(3362..4570)
                     /locus_tag="PLL28_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNF67996.1"
                     /translation="MRQTILLLGMLMMFGSINAQTSYPFIKYDTSRIIYPGDSASMLK
                     FFAKLDALKKGDTSRLTIAHYGGSHIQAGYWTEALADSFQALGGYVGGGAYLFPYRLI
                     KTNGPPFYRSHGTGEWKRCRAVPSEMCSGLGMSGISATTSDSIASFGFQLRPNDHINS
                     FTYIRVYHNFNPDYVIEVPKDFPATYLRHDYPELGYVQFHFQTPVDSVHFDIHRNSST
                     RSDFILRGWSIENENPGVYYASMGVNGASTRSYLQCTEFVKELGTLHPDLVIFSIGVN
                     DAQDADLVLPDFFARYDSICAIVRAINPDCAILFTTITDNYIKRKTKNTRSLKVNAQI
                     MAVAQKYNAGVWDMFTVMGGLGSIDKWYRNGLAAPDRIHFNAKGYGIAAGLMFSAIKD
                     SYMYNTTLSK"
     gene            complement(4573..6030)
                     /locus_tag="PLL28_01405"
     CDS             complement(4573..6030)
                     /locus_tag="PLL28_01405"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNF67997.1"
                     /translation="MSNITPLRILAFLAICFVALGAVSAVFPEKGIHLGKNVTLRFPT
                     LASIFESDTTSADISNIVSIDAGDDTTEVVAKVIDTANIKGLVQLDSGVAIVTPIQFG
                     TKEAMDNFYASLYKTASDGSRVRVMHYGDSQIEVDRITGYLRARLQSQFGGSGPGLIS
                     VMPIADFVAVKNSWSGSWDRYTTFTNRDKRVPHRSFGPNAAFSRFMPVRDTTHALGSQ
                     HSAWLKITTNKNGGARLANYRYVKLLYGNATDPVKVELLADDVLIATDTLSVGGDHNI
                     QEFALAAAPGNFQLNFEGRDSPDIYGLSLEGNGGVIVDNLALRGSSGNFFTGMNQSQL
                     KGYYDQMDVRLIIMQFGGNALPYLKDEAGCIKYAQSIESQINTLKRLAPGASILFVGP
                     ADMSVKEGTTYVTHPLLEILNTELKNAAFRAGAAYYDMYAAMGGKNSMVAWVNAGIAT
                     KDYIHFSPEGARKIAILLYQAMMKEYNNYVIRESI"
     gene            6377..7564
                     /locus_tag="PLL28_01410"
     CDS             6377..7564
                     /locus_tag="PLL28_01410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNF67998.1"
                     /translation="MTSLRAQQTWIRTGITDTILAIHGDGTLIWAATEADGVKSFNPA
                     TGAITTYNSAKSGFPDNDFRSILCVNGDVYAGSNNYGLFILHDEGWSSFDTTNSDIIG
                     NSIKDIMYVEADSSIWFATDKGLSILKEGTWTNYDSIASPLAGNALTCLYQSREGIIW
                     IGTKYAGLSKYDNGTWETFNYGNSGINNNAIRAITQDEDDLLYVADYLGVNRYDIVAD
                     NWLFVYNLFTVPMTSEKVNRMGFDDDDNFWMATHGGVTRADSTNHFTLYYDYNSNLPN
                     NVCDALYIDENNTVWVGTYGGLAFYTQEAFPNTITSSSMSIIPNPANTDITVTINQLA
                     EDIPTFCIYDLNGKNLENVIGVPALYGECAYTINISDLPSGMYLLCAIQSNERMFETF
                     VKL"
     gene            7782..8690
                     /locus_tag="PLL28_01415"
     CDS             7782..8690
                     /locus_tag="PLL28_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF67999.1"
                     /translation="MKTTHLFALILILFASMRASQAQITVFEYSKKEMAHNLSELKKR
                     NLIFVIRDSEIEQIPKYETALRNGWTLSEVEVVTSDELLYYEDSENYTFVTLDARAAV
                     AGKADMSTAVLTMMFKDSVTKKGPTNIKLAEIFMYADYKYFNTTYKDIITKEDSVIQI
                     HNYLPGFLQLYFQYIQKNLETGTSVKHFQKTETLNSNKTALFKNVLYIPDYCLKKFKL
                     IDPRANEYYEESEFRDKYFGKLAFVNSDSLTDLILRKEINFVLIYNMHYTQSFVNIFN
                     VQTGEMVHKNVYTGDYDLAPVRLKEI"
     gene            8696..9067
                     /locus_tag="PLL28_01420"
     CDS             8696..9067
                     /locus_tag="PLL28_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HNF68000.1"
                     /translation="MFKPVIDFHQSKHAMATYSDFEKIEIRTGTIISAEPFPAARKPA
                     YQLTIDFGALGIKRSSAQITKHYTAESLIGKQVVAVVNFPVKQIANFFSECLVLGAIE
                     ADGNVVLLQTERTVKNGLRIA"
     gene            complement(9072..9413)
                     /locus_tag="PLL28_01425"
     CDS             complement(9072..9413)
                     /locus_tag="PLL28_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HNF68001.1"
                     /translation="MAKQKLNLSGLVYSTNPDYKPESSEENHETLAPAQQNLRVWLEK
                     NHRGGKTATVIRDFVGNTDDLEALCKMLKSKCGTGGSAKDGEIIIQGDHRKKIGEILG
                     GLGYKYKLAGG"
     gene            complement(9417..10304)
                     /locus_tag="PLL28_01430"
     CDS             complement(9417..10304)
                     /locus_tag="PLL28_01430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNF68002.1"
                     /translation="MKKHILFIINPVAGVKRKDRIPRLIDKYLDHSHFDYEVIYTERR
                     GHATQIASEAAARGVHVVAVAGGDGSVNEAATGLIGTNTCLAIIPSGSGNGLARHLGY
                     SINIRSTINIINAYHVRKIDVCKVNGTMFFSLIGIGFDAFVAKVFSREETRGFLTYAW
                     SAIKSVSAFDAFEYSANLADRKIGGKAFMINVCNSNQYGYNVKVAPHASLSDGMLDVI
                     VLGDIPKWKIPLVVLQVFTRTHMHTKHFTIYKTPSLTIDTPQYAHLQIDGETAPKDKS
                     FKIEIMPEALQVLMHPDND"
     gene            complement(10301..12232)
                     /locus_tag="PLL28_01435"
     CDS             complement(10301..12232)
                     /locus_tag="PLL28_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNF68003.1"
                     /translation="MKEQLFHIEILLKRLGILLLLYMVCRILFFLFNMALFPHIPPGE
                     FLQLMLYGLRFDLSSIIYVNCIFIVLHVVPSPWFDKNWYQRMLKIIFYSFNGVGLLLE
                     SGDWIYYEYGLKRTSTHELGLTGDTNILPQVIKDYWIIFVIAFILILGVEYLYRKTEL
                     HRLKKVHFKLPRFIHYPYQIIIMVVVLTFSVIGARGGIQPEPISPEIATDYVDDGRLT
                     SLIINTPFSVIYAFGHRQLVEPVYYEDEQELAKQFNIFHDNALYYQWPDSIAKPSKPE
                     NVCVIVLESFSKEYIGYFNHGHGYTPFLDSLLTVGMAFDNAYSNGKSSNQGIVAVTSG
                     IPVLMEEPFISSIYRNNKFEGVGTLLKDVGYSSYFFHGANNGSMQFDRFMAQAGFDGY
                     FGRNEYGNDADFDGNWGIFDEPFLKWTANKMTTLKAPFYSEIFTISSHHPFTVPAQHV
                     GQFPKGPIPMLEVVGYADYALQQFFEEAKKQPWYNNTLFIITADHPGPPLPEHEFYQE
                     QVGAHSTWLLMYKPNGQFKGVNSEVVQQSDIMPTVLDYVGYKGKYMAFGNSVFDTTAA
                     RYAYNYHTSDYMLLDKDFALIFNGDRPVGLYDYKIDSTLSNNQMHEFPDITASMEDRI
                     KAIIQIHHHAMINNKLVAE"
     gene            complement(12282..13139)
                     /gene="nadC"
                     /locus_tag="PLL28_01440"
     CDS             complement(12282..13139)
                     /gene="nadC"
                     /locus_tag="PLL28_01440"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNF68004.1"
                     /translation="MSSVEIWDIIDFIDEALIEDIQEGDHTTLATIDHQAAGTAKLLV
                     KEPGIIAGVELAEQILRHVNPELEVDIFIPDGARVEPGDVVFHATGRVQALLVAERTM
                     LNFMQRMSGIATTTNLFVQTVRHTGAKILDTRKTTPGLRTFEKWAVRIGGGHNHRYGL
                     YDMILIKDNHIDFCGGITPAILKVKEYLRDNDLNLRIEVETRNLEEVAEVLRTGHVDR
                     IMFDNFDVDTMRQAVIEVAGQFETEASGGVNLTTVQAIAETGVNFISVGALTHSVKSL
                     DLSFKALIH"
     gene            complement(13132..13533)
                     /locus_tag="PLL28_01445"
     CDS             complement(13132..13533)
                     /locus_tag="PLL28_01445"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HNF68005.1"
                     /translation="MFKNNRTKLIAILAVVPFLIFATMQSELAGITSAIKSGDSKSLA
                     TYFDNTVEVKIANKEGAYSKSQAEAVVKDFFTKNPPKDFSFLHEGTSGGSNGHYAIGS
                     LSTDKGKFRTYVYMKKRADKFYIQELSFENE"
     gene            13792..15330
                     /gene="gpmI"
                     /locus_tag="PLL28_01450"
     CDS             13792..15330
                     /gene="gpmI"
                     /locus_tag="PLL28_01450"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNF68006.1"
                     /translation="MKSMKKVMLIIMDGWGHGQNAAASAIAQANTPNVNAYYKRYPNA
                     ELRTDGEFVGLPEGQMGNSEVGHLNLGAGRIVYQELQRIHMAIRNGELASNNTLLSAL
                     DFAKNNGKKVHFIGLVSDGGVHSHSNHLMALCDIAKSKGLTSDQVFIHAFTDGRDCDP
                     KSGVGFIRTLQQHLTNSAGTIASVCGRYYAMDRDKRWERIKKAYDLLVHGEGNATQDV
                     IKSIEQSYSDNVTDEFILPIVCTDAQQKPIATIAAGDVVICFNFRTDRCRQITRALTQ
                     EAFPEFDMTPIDLHYVTMTRYDHTFKNVGVIFESDDLSKTLGEILEANNKTQIRIAET
                     EKYPHVTFFFSGGREKEFSGERRIMIASPKVATYDLQPEMSAYLVKDAIVEAINNESP
                     DFICLNFANADMVGHTGVFSAAIKACEAVDTCVGEVIEAALSKNYAVILTADHGNSDV
                     EINEDGTPNTQHSTNPVPVFYIDKEPAFQQIRNGKLGDIAPTILTLMGIDIPKEMTGE
                     ILVS"
     gene            complement(15401..16768)
                     /locus_tag="PLL28_01455"
     CDS             complement(15401..16768)
                     /locus_tag="PLL28_01455"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNF68007.1"
                     /translation="MDFINNYIETNKERLLDELFALLRIPSVSADPKYKADVARTAHA
                     VADSLRAAGADNVRVNATEGHPIVTAEKIINPSLPTVLCYGHYDVQPADPVDLWDSPP
                     FEPVIKNGNIYARGACDDKGQVYMHVKAFEAMMQNNALPCNVKFIIEGEEEVGSPNLG
                     TFIKENKDLLSCDVILISDTSIIANDVPSVTVGLRGLTYMEVEVTGPNRDLHSGVYGG
                     AVANPINTLCDMISSLIDKDGHITIEGFYDDVALISDSERAEMAKTPFSLDEYKQDLA
                     INDVKGEKGYSTLERSSIRPTLDVNGIWGGYTGEGSKTVLPSKAYAKISMRLVPDQSS
                     EKIAELFDKHFNKIAPPYVKVKVTHHHGGEPVVVPLDFPPMQAAIMAMEKTFGKKPIP
                     TREGGSIPIVAMFEKVLGAKSILMGFGLNSDAIHSPNEHYGLFNYYKGIETLPWFFKY
                     YSELK"
     gene            complement(16797..18557)
                     /locus_tag="PLL28_01460"
     CDS             complement(16797..18557)
                     /locus_tag="PLL28_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68008.1"
                     /translation="MWRFIISPNDVMVSGRNDGLKNFFTLQAYLEQPASDGYIDFTAM
                     QYPYGDAVWYTDNSPFIAMPLRFIQLHFGNIAPYTVAIIQWIFLLGILLTPLTVIPVL
                     RRFLNNEWLVAIGAIVITWTSPQFLRFFVGNYNLSLTPVYFLVMLLLLRIYDNFQSRN
                     RKKLLLHAALIAIVIFLASGIHLYYVILLGLPAIAFLGILFLQQIRKSAEVLYFTAIP
                     SFAIAIATFGMMLLINTTDPWRSMRPTQLDGSNISAWEMRFMHLYKARDEVNLVPFIG
                     GKIHFDPENGVYLGGFFWYLLLMVFILIAFRFFRYRDVSFIKTLKPSPVGWIFILTAI
                     VAFFAGTGMHLHLIGRGNTMENIFNPLYYIGKIYPGVSQFRCIARMGWWLFYAAQIGI
                     LVAVERIILNQKKWIVNAVLCIGLSLLATDIYGIKQFTNSRYYPNYFNQKSLASLPDL
                     DYDAYQAILPIPYYHVGCEEYDFTIDDANEWSRYTYQLQLKSHLPLMSVKLSRTPQLF
                     ASQMMQLLLEPQSPGTLHEYLNDKPILVIYDPSIEEKQNLEPAATVLKESPGLITSEH
                     MVLIGSKGNVQYYAWYLKSN"
     gene            complement(18634..19500)
                     /locus_tag="PLL28_01465"
     CDS             complement(18634..19500)
                     /locus_tag="PLL28_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNF68009.1"
                     /translation="MHDHPVDILRLELAQPRLQGNKFYKLKYNIERFKQENCDTLLTF
                     GGVFSNHIHAAATACYDHGIRGVAVLRGEDDPFNPTLKFIRSMGMETIFVSRELYRQR
                     NNSDFLHHFSIDANKIYVLPEGGTNEFAVRGCSEILGDLADKYDRIFCAVGTGGTLCG
                     LICSPGLSATVTGISSLKADKDSLTPYIRQLCGDAGNKTQWDINFQYHFGGYAKYNDT
                     LMAFVEQFEHKYNIKPDPVYTAKVFYAVNDMISNKSISASERILILHTGGLQGWDGWH
                     YRFHHPKKQSHA"
     gene            19576..19649
                     /locus_tag="PLL28_01470"
     tRNA            19576..19649
                     /locus_tag="PLL28_01470"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19610..19612,aa:Ala,seq:ggc)
     gene            complement(19679..20794)
                     /gene="lpxB"
                     /locus_tag="PLL28_01475"
     CDS             complement(19679..20794)
                     /gene="lpxB"
                     /locus_tag="PLL28_01475"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNF68010.1"
                     /translation="MRYYLIAGEASGDLHGANLVRALSAKDPDAVFRGFGGDAMHEAG
                     VDIVTHYKETAYMGFVEVFMHLRKILNNIKSCKQDITGWKPDVVILIDYPGFNLRIAP
                     HVHEIGIKVFYYISPQLWAWKSSRVNIIKQYVDRLFVILPFEEAFYQKFNYPVSFVGH
                     PLLDAIASNKGNEHFREQFQLGDKQLIAVLPGSRKQEITVKLPVMLSMAKYYPEFVFV
                     VACAPGMDDSFYAGFEKRENIIYISGSTYDILRNAYAAMVTSGTATLETALFRVPQVV
                     CYKGSVISYHIAKRLVKIKYISLVNLIADKQVVPELIQHDFNHDSLRTHLDLLLKDAA
                     YRNTMLSEYDKLYTVLGGVGASARTADEMMKLLAEGK"
     gene            complement(20803..21120)
                     /locus_tag="PLL28_01480"
     CDS             complement(20803..21120)
                     /locus_tag="PLL28_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68011.1"
                     /translation="MSKLQEDNVFVGMAVAVFFMVTTYYAFFQLNESLSGNITIKGAI
                     FTGVTERFIATLAVFFNILPFVIYMRVRKDNCMRGVGIITVLSAMFILVYFYIQHHST
                     IFQ"
     gene            complement(21126..21968)
                     /gene="surE"
                     /locus_tag="PLL28_01485"
     CDS             complement(21126..21968)
                     /gene="surE"
                     /locus_tag="PLL28_01485"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNF68012.1"
                     /translation="MQQNNNKPIILITNDDGIHAPGIHALVEAMRPLGTIVVVAPDKP
                     QSGMGHAITLNEPLRLKKSNLFGDIEAYACTGTPVDCVKLAKDKILHKKPQLCVSGIN
                     HGSNSSINIIYSGTMSAAMEAGIEGIPAVGFSLLNYAIDADFTSAAHYAAKIAEQVLQ
                     HGLPQNTLLNVNIPYLPIDEINGMRICRQAHAKWEEEYDERLDPHGRPYYWLTGNFVN
                     YDGGDDTDEWALSHGYVSIVPVQYDLTAHQAIAFFHKNWSLYQTKSPHDIHLDNSDTF
                     NDLG"
     gene            complement(22037..23542)
                     /locus_tag="PLL28_01490"
     CDS             complement(22037..23542)
                     /locus_tag="PLL28_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68013.1"
                     /translation="MRMQAIYRYCLIIILFSGISPLHAQNEILAKADKYYNNGIYPEA
                     AALYIDHLEQQYDYIVNVKLADCYYRMEEWSEAEHWYGVIVSHNIADAGTLLRYATLL
                     KMNGKYRSAKTYFLKYAAYKPDGYYLASTCDWALANMNKPSQYYIDTLPFNTKGSEMT
                     PTIYKNGIIFAKSNGTKINNNTGLPYYDLYSADVRTDGSWITNKLDHVNSPYHDAAPY
                     YDGASKGLYFTRSNHYKNRTVVSIDGQVRLELFYAPYVDSKFGTPHPLNVNSKIYSVG
                     HPATTPDGNILIFASDKQGGKGGTDLYYCMKRNGNWSEPKSMGDVINTAGDELYPFMT
                     ADGTLYFASNYHPGFGGFDLFKCEREGDHWSTPENLGMPVNSPQDDYGFTMKNGVGYF
                     SSNRPGGMGGDDIYQVTQMAQIASVFVYDNNKNAIYKARVTFFESPNSQVICETDPNG
                     YGDVSTLSGVSTSIKISKEGYLDKVINNLGGLRSSNGVLPVELTPLIGGNE"
     gene            complement(23542..24501)
                     /locus_tag="PLL28_01495"
     CDS             complement(23542..24501)
                     /locus_tag="PLL28_01495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNF68014.1"
                     /translation="MKKLYQSTLLLLMLCSANALYAQLPPLFEQYHLNQLVFNPAYAG
                     SKGGIEAGFFLHRHSTNVQGAPGTESFTLHTPLANDNVGLGLKIYHDKLGVTNNSFVG
                     LDYAYRMHISDHLTAAIGIEGSIANYKVDYSQLDAFINGDPTFTKASESYWSPDAGLG
                     VYLHSDIYYVGLSVMSLSGLSANGNDNPDVAQDEKFDKVATVYGTLGTLIKINDKLTI
                     KPDLLIKITDSIPAQMDVNLNFIYNNVLLVGGGYRTNGSISFVGQYIFSTDNKITGHE
                     AGLGYSYNTMLADDKVFISPSHEVFLTYRFYKHNNKFVNPRFF"
     gene            complement(24498..25736)
                     /locus_tag="PLL28_01500"
     CDS             complement(24498..25736)
                     /locus_tag="PLL28_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNF68015.1"
                     /translation="MMQRYAGIITSILFSILGSSSMVHAQSYDAAPDFYEINEDVPDV
                     TMDVMANDLNTTGVTDVYLNIATLPLHGTVTVDAVNDLLIYSPNDDYYGTDNFEYTGC
                     ANTDPDICGTTSVIITINPVPDKPIAVDDAVETYVNTPLNIEPLLNDIDVDNEGLEFT
                     IIDDADYGATTIIFGEAVFYEPFPCVYGEDCFIYKICKIGSDIYCDTATVCVDVQTTN
                     FNPPSALNDTATVVVIDTIDIPVLLNDFDGDGDAMIVSEIIADGMTGTATINADNTIK
                     YIPFVVGTDDFRYVVCDNNCPPYCDTAYVHMKIEEPEIPAEAIHVPNSFSPNGDGIND
                     MLTIEGLESGIKYNVNIYDRNSSLVFEKEKLATSDVWDGTSNVEFISSSGNVPEGTYF
                     YVIRIEGLPDPLSGFIVVKR"
     gene            complement(25754..27382)
                     /gene="pruA"
                     /locus_tag="PLL28_01505"
     CDS             complement(25754..27382)
                     /gene="pruA"
                     /locus_tag="PLL28_01505"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HNF68016.1"
                     /translation="MSNAIFRIPKALNEPVKGYAPGSPERKALKQAIADLKAISVDIP
                     MFIGGKEVRTGKTVAIHPPHEIAHTLGHYHQGGAEHVKMAIDAALTAKQAWENTPWED
                     RAAIFLKAAELLAGPYRYKMNAATMLGQSKNPFQAEIDSACELIDFWRFNAEFMQQVY
                     SDQPESAPGIWNRMEHRALEGFVFALSPFNFTSIGGNLPTAPAMMGNTVVWKTSNTQI
                     YSAWVIMEILREAGLPDGVINLVHVSGSEAGKVIFSHPDFAGIHFTGSTEVFRTIWKT
                     IGDNIHVYKTYPRIVGETGGKDFVIAHPSAHPKQVATALSRGAFEFQGQKCSAASRAY
                     IPSNMWEEVKRYLVTDLAEMKMGGIEDFSNFINAVIDEKAFDSISGYIAKAKADGQEI
                     IAGGTYDKSKGYFIAPTVILAKDPSYRTMCEEIFGPVLTIHVYDAEKWSETLQVVNNT
                     SPYALTGAIFSQDRYAVVEATVALRHAAGNFYINDKPTGAVVGQQPFGGARASGTNDK
                     AGSILNLLRWVSPRTIKENFVPPVDFRYSFLNEE"
     gene            27575..28504
                     /locus_tag="PLL28_01510"
     CDS             27575..28504
                     /locus_tag="PLL28_01510"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68017.1"
                     /translation="MHKLSTYSFGIVFSLLLLLLPSWASAQSRTQQFNQLLDKNDTAR
                     AQVLLTQWKKDVPGDPEWYVARYNFLVWSSMKSIVTIGSNPKGNNYMEITPTDTSADA
                     TPQFMYADVYYQPKQVASAIACIDSAIALFPDRLDLRFGKIYLLGETQSYQAFADEIV
                     KTIHYGATFNHNWKWTDGLAIDGGIASFNESIQSYIVQLYDTNVDSLGVQIGQIATAV
                     LQYDPNNIENLSNLAISYMLASNYEEALTPLLKAETVDPTDAIVLSNIAFCYHRINNK
                     EQAIAYYEKSIKYGDREMKAFSYEQIQLINSEK"
     gene            28506..29027
                     /locus_tag="PLL28_01515"
     CDS             28506..29027
                     /locus_tag="PLL28_01515"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF68018.1"
                     /translation="MDWVIETPRLLLRELNTDDAQFFFDLNNDPEVIRYTGDVAFESI
                     DAARQFLAGYDHFKKYGFGRWAMATKSEHKLVGWCGLKYSSDANEYDLGFRLFKNEWG
                     KDFATEAGIACLTAGFQKFNMPMIVGRALKANIGSIRVLEKCGMRYWKDVEEHDNEAV
                     VYRCFPEDLNNLL"
     gene            29052..30194
                     /locus_tag="PLL28_01520"
     CDS             29052..30194
                     /locus_tag="PLL28_01520"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68019.1"
                     /translation="MRKLISFLSCIFLAGIMHAQVTIIDSIYSGGMYRNYRMYIPTAY
                     DGSEAVPLLFNLHGYSSDNFGQALYANFNPIADTAGFITVLPNGTFDAFGLRYWNCFV
                     APGIGVDDVQFLSDLIDSVAAEYNIDLNRVYSTGMSNGGFMSYTLACELSERIAAIAS
                     VAGTIDKDRLSYCNAQHNVPVLHFHGTADPTVPYGDNLAFFSVDSVINYWVAHNHTDA
                     TPVFTEVPDIVTTDGSTAEHYVYGNGWTDSRVELYKIIGGEHTWPGTAITFLGITNQD
                     VNACKEIWRFLSQYRLDELSSVDPLTDNDLVQINPNPADQFLVINSKFSINGVEIINM
                     NGQIIKQFNPAGATQVNIPLSELAQGLYLIKISTGDTYITQRFIKQ"
     gene            complement(30224..30715)
                     /locus_tag="PLL28_01525"
     CDS             complement(30224..30715)
                     /locus_tag="PLL28_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68020.1"
                     /translation="MKIFLTILTCGLIVQAQAQTIVPISSAEQSYEVLDLTKEAKITW
                     GGYEEIGVATRSETDGALNSKAIVVAVGDNPGFDNKPYAAQVCENSTAGGHDDWYLPA
                     KDEAALIYLNIDKFNWSDSYTLWTSTEAAGTQAVSLYTYNGTWYNVQKVDSYNMVCMR
                     KTK"
     gene            complement(30797..31840)
                     /locus_tag="PLL28_01530"
     CDS             complement(30797..31840)
                     /locus_tag="PLL28_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HNF68021.1"
                     /translation="MSHKPVEYTEYLQLDKILNAQSLESDKAGAHAHDEMLFIIIHQA
                     YELWFKQVHFEVDSALQVMGKPEVNDNSPELQTVVHRLDRVVTILKVLVHQIDILETM
                     TPLDFHEFRDFLRPASGFQSWQFKMLEAKLGLKFDNRYGKEYYTSMLKPQYVDLVKDV
                     ESQKSLLEMLDDWLARMPFFSDDNLWKSYQPASQNDSTHHVFWNDYLNAYSKTLVEGE
                     QHNKEIFASIFLGEGKSEHTSLSNKAMRNALFISLYRGYPLMHLPFQLMQHLLDIDEQ
                     MATWRFRHINMVHRMIGSRVGTGGSSGKEYLQGALNKHYIFKDFAVLSSFLIQRNKLP
                     HLSSELNHKLGFI"
     gene            32263..34704
                     /locus_tag="PLL28_01535"
     CDS             32263..34704
                     /locus_tag="PLL28_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNF68022.1"
                     /translation="MAETKKSGKKAKKASNSAPFYKDERILKVTGVFLLLLSFFSGVS
                     FVSYLFTWKADQSLIWDHPWAQVFNSGITVENHLGNLGAVLSHQFFFCWFGVASFFFV
                     VWFFTLGVNFFFSRQIFNNVKLIGQGMVVMAWISTMLGFFMYKAEFPYGGGFGKGTAF
                     HLESYIGKVGAILALTAAMVVYMIIFTKFDFGFLLNMFRKKEVIPAVDPFTEDDMPVQ
                     TEEIPEVEKEMINVPPVYTSNVDITPLQGEVDDVVMELKKVEPVSEQSMPLEIEEPET
                     IVIAGDEEPLLDHQDHLSLDIETPVEEQIAAENLNYHFDPVLDLPNYKYPELDLLEEY
                     ASETVSINTEELERNKDQIIATLNNYNIQISKIKATIGPTVTLYEIIPAAGVRISKIK
                     NLEDDIALSLAALGIRIIAPIPGKGTIGIEVPNVNKETVSMKSQIGSDKFQHAKMDLP
                     VCLGKTISSENYIADLTRMPHLLMAGATGQGKSVGINAILISLLYKKHPSQLKFVMVD
                     PKKVELSLFSSIEKHFLAKLPGEEDAIITDTRKVVNTLNALCIEMDTRYELLKNAQVR
                     NIKEYNAKFMKRKLNPKEGHMFLPYIVLVIDEFADLIMTAGKEVEMPIARIAQLARAV
                     GLHCVIATQRPSVNIITGTIKANFPARIAFKVASKVDSRTILDNSGAEQLVGRGDMLL
                     AIGSDMVRLQCPFVDTPEVDKVVDFISDQIGYPEAFMLPEFIDEQAQKEKMEIMDGER
                     DPLFAEAAKIIVQHQQGSTSLIQRRLKLGYNRAGRLMDQLEAAGIVGQSAGSKPREVL
                     YSDLAQLEQYLQTLD"
     gene            34758..35408
                     /locus_tag="PLL28_01540"
     CDS             34758..35408
                     /locus_tag="PLL28_01540"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HNF68023.1"
                     /translation="MKKISIMVFALVMTAGIAHAQTSDPAAVKLLKSVSQKYSGYKTM
                     QMDIALTIENLEAKTKETKNGKVSAKGNMFKAEMGNQIIMSDGKTLWTYLKDVNEVQI
                     NNFEKGAGIMTPNDIFKIAEKDYLAYMGEKVTEGGKSLQIIELTPKDKTLSFSKVKMY
                     VDPSDNSIKKGVVFDKNAIHYTYVISNFKSNVEMNDTNFKFDKSKYPGCEVIDLRE"
     gene            complement(35555..>36785)
                     /locus_tag="PLL28_01545"
     CDS             complement(35555..>36785)
                     /locus_tag="PLL28_01545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNF68024.1"
                     /translation="AVHPNFNDNVYVSICDDDTYTLPDGTVTNIAGIYPTMFATAFGC
                     DSLITTYLYVFPTYDLTSTVNICSGDTYILPDGIETGVAGVYIANLLTDKGCDSIITT
                     TLNVDPTYFPTFNPEICEGETYTLPDGNIVNASGTYVTSYSTVAGCDSIITTNLTVHP
                     LPVIIFTIDDVVCVEDAALTLNAYPSGGTYSGTGTTGTSFDPATAGVGGPYAITYDYT
                     DVYGCSSSATVSISVDENTATAWGDTTIYAGEPAVLHSEAGGDYNWTPPTQVICTWCA
                     ETNAYPLESILYTMVSTNENGCIASDDVYIEVLPNPGNASYIPNTFTPNGDNFNDYFF
                     AYGYNLASIRSMRIYDRWGALIFEKDNIPATNDTEGWDGTFNGESLNNGVYAYIMELT
                     FENGITTQYAGNITLIR"
BASE COUNT        11167 a         7731 c         7206 g        10681 t
ORIGIN      
        1 cttcggcaat tgcgcgtaat gcttttgcat tatttaattc atccggagta agattttctt
       61 tttccgaaag actgccatca cctgatattg gtgcttgaat aagccgttta agagaaatac
      121 ccggacgttc aggtacgagt tttgataagg cattactgtc ggcaattggc aaatccatta
      181 cccgggcaac atctttgata ctagtttttg ctgccattgt gccgtaggta accaattgcg
      241 ctacctgatt tttaccatat ttatctacca cataatcaat aactttttga cgtccatcat
      301 catcaaagtc cgtatcgata tcgggcattg acttcctgtc gggattgagg aagcgttcga
      361 aaagtaaatt atattttatc ggatcgatat ttgtaattcc aatacaatat gccacaactg
      421 atccggctgc agatccacga ccggggccaa caaaaacccc gagttctctt cctgcgcgaa
      481 tgaaatccat cacaatcagg aagtaacctg cgaatcccat tgttttaatg gtaaacaatt
      541 caaaattgat gcgttcttct gtttccgcat ccattgtgcc atagcgctta tgagcacctt
      601 ccatggttag atgcttcaag tattcccact gattagttgt atcgtcacca tgaattttaa
      661 atgcatccgg aatggggaat gccggcaaca agatatcacg cttaagatcg agcagttcta
      721 ctttatctac aatctcattt gtattgtcca gtgcctgtgg aacatcgtgg aacaatgcgt
      781 tcatttcaga ttgtgtttta aaataaaact gatcattata aaatgcaaag cgcttccctt
      841 taatgatcat atcatcatca ccgaaatcct tcatcttcgg tgtcgcctgc ttttcacctg
      901 tattgataca taataaaata tcatgtgcat tagcatcttg ttgatctaca taatgcgcat
      961 cattggtggc gattactttt acattatatt tatcggcaag ctcaagtaaa atattattga
     1021 gtgtttcctg ctcaggaata tcatgtcttt gcagttcgat ataataatct tcgccaaaga
     1081 tatccagcca ccatttaaaa actttctccc cttcttccct accctttttc aaaatcgttc
     1141 tgggcacctc cgctgcaaga cagcatgtgg tagcgataag gccttcgtga tatttttcaa
     1201 tgagtgtttt atcgatgcgt ggatattttc cgtacatgcc ctccatatat cccagagaac
     1261 acattttcac caggttttta tatcctgttt cattttttgc aagtaatacc tgatgatacc
     1321 ggatatcctt ttcttctctt gaaaattgtt tccttgttct gtccgcaact agataaaatt
     1381 cacaaccaac gataggtttt actttcacct ttccatcttc gcccttatgt ttataagctt
     1441 cggcaacaaa ttgaaatgcc ccgaacatat taccgtggtc agtaatagcg agtgcaggca
     1501 taccatcggc ggcagccttt ttatataaac gaccaatgtc actggcgccg tcgagcagcg
     1561 agaacatggt atggttatgc agatgcgaaa aagtggacat agcggattgc gaaattatat
     1621 ctgagccact taagatacac cttttcgggg acagcttttt cccaccttgt gttaataaat
     1681 attcaggtgc ttagaattga aaataaatga agggctgcat gtcagcactt acagcctggt
     1741 agagcaagaa gatataacct attaaaattg cgaccaaagc cggttctgag cgcgtagcaa
     1801 accactcacg gtattgttgt tttgtgcttt ccggaatcca gtggataaga tagccaacta
     1861 caatcactga taataccgtg cgataccccc acaatacatt ttctatcaat tcgccaccga
     1921 aatgatgact gatgcgatca aaaatgatgc ctaccgtatc gaggctatcg cttcggaacc
     1981 aaatccgcgt aaaactgatg aataccaatg ttagaaaaat cgcaaatgct ctatatccga
     2041 cacctgaagt atttttgatt ggattaattc gctcccagcc tttgtgcaac atcaatccta
     2101 agccattcaa cccgccccaa ataataaaca tccagctgct tccatgccat aaaccaccta
     2161 gcaacatggt aaccaaaata ttgatattgg tatttacaga atgccgaaat tttggaaata
     2221 ttcttgcgat taaaattaat actaaagcaa tcgccaacaa gatgtaaatc aaggtgacat
     2281 taccatataa taataccata aatattccca gaatcgatat cgcaacatag gaacccactg
     2341 ttccatttct gttccctccc aaaggaatat agaggtattc ttttaaccag ctcgacaagg
     2401 atatatgcca acgcttccaa aattcacttg tgctttcagc tttgtacggc gatctaaaat
     2461 tcgttttcaa tctgaatcct aataataatg caatgccaat ggccatatct gtatagccgc
     2521 tgaaatcggc atatatctga aggctgtatc cgaatatgcc aagcaccact tctacgccgg
     2581 agtacaattc aggatgcgag aatatacgat ccacgaaatt gacaccaaca taatcggcca
     2641 ttatcttttt gcccaatcca ttcaatatcc aaaaaaatgc cattccatac tcccttttcg
     2701 aaagattata tggctgataa atctggtaaa tgaaatcatg tgcttttaca atcggccctg
     2761 ccaccaggtg cgggaaaaaa caaacaaaaa atccataatc gaatatgttt ttcacaggct
     2821 caagtttcct gcgatacata tcgatggtat acgacagcga ttgaaaagta aagaatgaaa
     2881 taccgattgg cagtatgagt ttatctactg agaatgaagt accaaaccat gtatttgtaa
     2941 aatgcgcgaa atgattaaag aaagttatat ccgtgccggc aacttcattt aaattttcgg
     3001 taaagaaaaa cgcatattta aaataacaaa gcaaagttaa attcaacacc acactgaatg
     3061 tgaggaacat ccttcgtttc cagggcgttt ctgtctttgc aagcagtcga ccccagttat
     3121 agtcgctgat gatagtaaaa atgaggagga taagaaagac accactggtt tgataataga
     3181 aaaagaaact cgctgccagc agataaatgg ttcggacatt tactttttta tatacgaatg
     3241 aatatccaag caacacaact gcaaagaatg cccagaaaaa caaacgtgta aagatcaacg
     3301 gattttccgg atcataagat aaccaatgta caatattgct aaaaatgtta ttcaatgcga
     3361 attactttga taatgtggta ttatacatgt agctgtcttt aattgcactg aacatcaagc
     3421 ctgccgcaat tccataccct ttagcattaa aatgtattct atccggcgca gccaaaccat
     3481 tgcgatacca tttatcgata ctccctagtc cgcccattac ggtaaacata tcccaaacac
     3541 cggcgttata tttttgtgca acagccatta tttgtgcgtt cacttttaga ctcctggtat
     3601 tttttgtttt tcgcttaatg tagttatctg tgatggtagt aaaaagtatc gcgcaatcag
     3661 gatttattgc tctcactatg gcacaaatgg aatcataacg cgcaaagaaa tcaggtaaaa
     3721 caaggtcggc atcttgggca tcattaacgc caatagaaaa aatcaccaaa tctggatgta
     3781 aggtgccaag ctctttaaca aattcggtgc attgcaagta agaccttgtg ctggcgccgt
     3841 ttacacccat cgacgcgtaa tatactccgg gattttcatt ttcaatactc catccacgta
     3901 aaataaaatc agatcgtgtt gatgaatttc tatgtatatc gaaatgcaca gaatctacag
     3961 gtgtctgaaa gtgaaattgc acatatccca gttcagggta atcatgtcgc aaataagtag
     4021 cagggaaatc cttcggcact tctataacat aatcaggatt aaagttgtga tatactctga
     4081 tataggtaaa tgaattgata tggtcattag gtcgaagttg aaatccgaat gatgctatgc
     4141 tatcacttgt tgttgcagag attccactca ttcccaatcc gctgcacatt tcagaaggca
     4201 ctgctctaca acgtttccat tcacctgtgc catgagagcg atagaacggc ggtccatttg
     4261 ttttaattag ccgataggga aataaatatg ctccacctcc tacatacccg cctaatgcct
     4321 ggaaactatc cgcaagcgcc tctgtccaat agcccgcctg aatatgagat ccaccataat
     4381 gcgcaattgt cagtcgagat gtatcacctt tttttagggc atctaatttg gcaaaaaatt
     4441 tcagcatact cgcactatcg cccggataaa tgatcctgct ggtatcatat tttatgaacg
     4501 gataacttgt ttgtgcatta atgcttccaa acatcatcaa cattccaagt agtagtatcg
     4561 tctgtcgcat gttcatatac tctctctgat cacataatta ttatattctt tcatcattgc
     4621 ctggtataat aaaatggcaa ttttgcgtgc tccttcaggg ctaaagtgaa tatagtcttt
     4681 ggtagcaatt cctgcattca cccatgccac catactattt tttccgccca ttgcagcata
     4741 catatcgtag taagctgcgc ctgcacgaaa ggctgcattt ttaagctcag tgtttaatat
     4801 ttcaagcagg ggatgtgtca catatgttgt tccttccttt acactcatat ctgcggggcc
     4861 aacaaataat atactcgcac ccggagctaa ccttttcaag gtattgatct gcgattcaat
     4921 agattgagcg tatttaatac atcctgcctc atccttgaga tatggtaacg cgttacctcc
     4981 gaattgcatg atgatcaaac ggacatccat ttgatcatag taacctttca attgcgactg
     5041 gttcatgcct gtaaaaaaat tcccggagct tccacgcagg gcaagattat ccactattac
     5101 accaccgttt ccctccaaag acaagccata aatatccgga ctgtcacgac cttcaaaatt
     5161 taattggaaa tttcccggag ctgcggcgag agcaaattcc tgaatattat gatcaccgcc
     5221 aacgctcagt gtatctgttg ctattaatac atcatcggca agcaattcaa cttttacagg
     5281 atctgtagca ttgccgtaca ataatttgac atatcgataa tttgccaatc tggcgccacc
     5341 atttttgttc gtagtgatct ttaaccaggc actatgttgg ctaccaagag catgtgttgt
     5401 atcccttacc ggcataaacc tgctaaatgc tgcattagga ccaaaacttc gatgcgggac
     5461 tcgtttatct ctattggtaa aagtggtata tctgtcccaa cttccactcc agctattttt
     5521 taccgcaacg aaatcagcaa taggcataac ggagattagt cccggaccgg agccgccaaa
     5581 ttgggactga agcctggctc tgaggtatcc ggtaatccta tcgacctcga tctgtgaatc
     5641 gccatagtgc atcaccctaa cccgagagcc atcagatgct gttttataaa gtgatgcata
     5701 aaaattatcc atcgcttctt ttgtgccgaa ttgaattggt gtaacgatgg caactcctga
     5761 atccaattga acgagtcctt tgatatttgc agtgtcaatc actttggcaa caacttccgt
     5821 ggtatcgtcg ccggcatcga ttgagacaat attactgatg tcagcagaag tagtgtcaga
     5881 ctcaaatata gatgctaacg taggaaaccg caaggtaaca tttttcccta agtggatgcc
     5941 tttttccgga aatacagcac tcacagcgcc cagtgcgacg aaacatatgg ccagaaatgc
     6001 caatatccta agaggcgtga tgttgctcat tgtgaagagg ggataaaatt acggaagtgg
     6061 gtcatgtgtt gtgtgcattc accaacattt gaatgcacaa ttgttgaatt aaacggacat
     6121 tcatgtcgct tattatattt aactgcaaat aatatcgact ttaacttctg cttcaattcg
     6181 gtaattcagg ttgaagaaca ttttgaatat taaaaaacat aatttaagga attattttca
     6241 aacacttgcc gaataccgct cccgattccc tttaaccttc gtctagaatt gcattgtttc
     6301 acacaataat gcgatttttg catcgttcac actcctacag atgaaaacct ttgggctgat
     6361 ttcaatactc gtttgcgtga caagtctccg tgcgcagcaa acctggatcc ggacaggtat
     6421 taccgacacc atcttggcca ttcatggtga tggaaccctt atctgggcgg caactgaggc
     6481 cgatggagtc aagagcttca atcccgctac aggggcaatt actacctaca acagtgcaaa
     6541 gtcaggcttc ccggacaatg attttcgaag tattctatgt gtcaatggcg atgtatatgc
     6601 cggatcaaac aattatggtc ttttcatttt acatgatgaa ggctggtcat ccttcgatac
     6661 cacaaattca gacattatcg gaaacagcat aaaagacatt atgtatgtgg aagcagatag
     6721 cagcatttgg tttgcaaccg ataaaggact gtcaatccta aaggaaggaa catggacaaa
     6781 ctacgattct attgcttcac ctttggcagg caatgcgctt acatgcctat atcaaagtag
     6841 agagggaatc atttggattg ggacaaaata tgcaggttta tctaaatatg acaatggcac
     6901 ctgggaaacc tttaattatg ggaattcagg tataaataac aatgccattc gggctattac
     6961 tcaagatgag gatgacttgt tatatgtagc agattatctt ggtgtcaaca gatatgatat
     7021 agttgccgat aattggttgt ttgtatataa tttatttact gtacccatga catcagaaaa
     7081 ggtaaacaga atggggtttg atgatgatga caatttctgg atggccactc atggtggtgt
     7141 aacacgtgcc gacagcacca atcactttac tttgtactat gattataatt cgaacctgcc
     7201 aaacaatgtt tgcgatgcct tgtatataga tgaaaacaat actgtatggg tgggaactta
     7261 tggtggtttg gcattctata cccaagaagc tttccctaat acaattacct cttcatcaat
     7321 gagcattatt ccaaaccctg caaatacaga tattactgtt acaattaacc aactggccga
     7381 agacatacca accttttgta tttatgattt aaatggaaaa aacttagaaa acgtaattgg
     7441 agttccggca ctgtatggag aatgtgcata cacaattaat atttcagatt taccatccgg
     7501 catgtatctg ctttgtgcca ttcaatcaaa cgagcgcatg ttcgaaacat ttgttaaatt
     7561 ataaagtcgt attttgttga caaactgtac ttaataaatc gcaatcagga ttatgattct
     7621 gattacgcca accaacttgg caaacggttt acacctctat gtatacctaa aaacttagga
     7681 atagaaatct agattactct tccaacttat accaaataat atattatttc ttgatcagac
     7741 aaactattgc taaatttcgt gcagattaca aagttaaaag catgaaaact acccatttgt
     7801 ttgctctaat actgatcttg ttcgcatcaa tgcgagcttc acaggcacag attactgttt
     7861 ttgaatactc caagaaagaa atggcacaca acctttctga gcttaagaaa cgcaatctca
     7921 tatttgttat acgcgattcg gaaattgagc aaatccccaa atacgaaaca gcgctgcgca
     7981 acggctggac attaagtgaa gttgaggtag taacaagtga tgaactttta tattatgagg
     8041 acagtgaaaa ctatacgttt gtaacgttgg atgcacgggc agccgttgcc ggaaaggccg
     8101 atatgtccac tgctgtatta accatgatgt ttaaagattc agtaactaaa aagggcccca
     8161 caaatatcaa gctggcagag atttttatgt atgcagatta taaatacttt aatacaactt
     8221 ataaagacat cattactaag gaggattctg ttattcaaat tcacaactat ttaccgggtt
     8281 ttcttcaatt gtactttcaa tatatacaaa agaatctaga gacaggaacc tctgtcaaac
     8341 atttccaaaa aacagaaacc ttgaacagca acaaaacagc cttgttcaaa aacgttcttt
     8401 acatacctga ttactgccta aaaaaattca aattaataga cccacgtgca aatgaatatt
     8461 atgaagaatc tgaattcaga gataaatact ttggcaagct agcattcgtc aattccgact
     8521 cactcaccga cctgatactt cgcaaagaga taaattttgt attaatctat aatatgcatt
     8581 atacgcaatc ctttgtaaat atttttaatg tgcaaaccgg agaaatggtt cacaagaatg
     8641 tatataccgg cgattatgac ctcgcgcccg taaggcttaa agaaatttaa tattattgtt
     8701 caaacctgta attgatttcc atcaatccaa acatgctatg gctacctatt cagactttga
     8761 aaaaatcgaa ataagaaccg gcactatcat ttcagccgaa ccttttcccg ctgcaagaaa
     8821 acccgcatat cagcttacca tcgattttgg tgctttggga ataaaacgca gcagcgcaca
     8881 aataacaaaa cactatactg cagaatcgct tattggcaaa caggtagttg cagtcgtaaa
     8941 tttccccgta aagcaaatag caaatttttt cagcgaatgt ctcgtcttag gcgcaattga
     9001 ggctgatgga aatgtagtat tgttacaaac agaaagaacc gtaaagaatg gccttcgcat
     9061 agcctaatcg attaaccgcc tgcgagttta tatttatatc ccaagccgcc taaaatttct
     9121 ccaatttttt ttcgatgatc tccctggata ataatctctc catccttcgc acttccaccg
     9181 gtcccacatt tcgactttaa catcttacac aaagcctcga ggtcatcggt attccctaca
     9241 aaatccctaa ttaccgtagc agtctttcca cctctatgat tcttctccag ccatacgcga
     9301 agattttgtt gtgcaggtgc aagggtttca tgattttctt cggatgattc cggtttataa
     9361 tcaggatttg tagaataaac cagtcccgac agattcaatt tttgttttgc catacattaa
     9421 tcgttatcag ggtgcatcaa tacttgtagt gcttccggca taatctcaat tttaaaagat
     9481 ttatcctttg gtgctgtttc tccgtcaatt tgcaagtgag catattgagg cgtatctatt
     9541 gttaaggaag gtgttttata aatagtaaaa tgcttagtat gcatatgcgt tctggtgaat
     9601 acctgcaata ccaccaatgg aattttccat ttcggaatat cacccagtac gatcacatca
     9661 agcataccat cgcttaaact ggcatgtggc gccactttta cattataacc gtattgattt
     9721 gaattgcaca cattaatcat aaacgctttg ccaccaattt tcctatcggc aagattggca
     9781 ctatattcaa aagcatcaaa tgctgaaaca cttttaattg cactccaagc gtaagtcaga
     9841 aatccacgtg tttcctcacg gctaaacact ttagcaacaa atgcatcaaa accaatacct
     9901 atcaagctga aaaacatggt gccatttacc ttgcacacat ctattttacg aacatgatag
     9961 gcattaataa tatttatagt cgagcgaata ttaattgagt atcccagatg tctggcaaga
    10021 ccattaccgg atccgctagg gataattgcc aaacaagtat tcgtgccgat caaaccggtt
    10081 gcggcctcat ttacacttcc atctccacct gcaacggcaa ccacatgcac accgcgtgct
    10141 gcagcttcag aagcaatttg tgtagcgtga ccccgacgtt cggtatagat tacttcataa
    10201 tcaaaatgtg aatggtctaa atatttatct atcaaacgag ggatacgatc cttgcgtttg
    10261 accccagcaa ccgggttaat aataaacaga atgtgctttt tcactctgcc accagtttgt
    10321 tgttgatcat agcatggtgg tgtatctgaa tgatagcctt tattcgatct tccatgctcg
    10381 cagtaatgtc tggaaactca tgcatctgat tattactcaa agtgctatct atcttataat
    10441 catacaaacc caccgggcga tcgccattga atatcaaagc aaaatcttta tccagtaaca
    10501 tataatccga ggtatgataa ttgtatgcat atcgcgctgc ggttgtatca aaaacagaat
    10561 taccaaaggc catatatttt cctttatagc caacataatc taaaaccgta ggcatgatat
    10621 cagattgctg aaccacctca gaattcactc ccttaaattg cccattgggt ttgtacatca
    10681 acagccacgt agaatgcgcc ccaacttgct cctgataaaa ttcatgttca ggcagcggag
    10741 gtccgggatg atcggcagtg atgataaata atgtgttgtt ataccaaggt tgctttttcg
    10801 cttcctcgaa aaactgctga agtgcataat cagcatagcc aacaacctcc aacatcggaa
    10861 taggaccttt gggaaactga cctacatgct gtgccggtac tgtgaatggg tgatgcgatg
    10921 aaattgtaaa tatttcagaa taaaatggcg cttttaaggt tgtcatttta ttggcagtcc
    10981 atttcaaaaa cggctcatca aatattcccc agttaccatc aaaatctgca tcattaccat
    11041 attcatttct tccgaaatat ccgtcaaatc cggcctgagc cataaacctg tcgaactgca
    11101 tggaaccatt attcgctcca tggaagaaat aggatgaata gccgacatct ttcaataagg
    11161 taccaacacc ttcaaattta ttatttctat aaatggatga aataaagggc tcttccatta
    11221 aaacagggat tccacttgta acggcaacaa tcccttgatt tgacgatttc ccattgctat
    11281 aagcattgtc gaatgccata cctaccgtta ataaagaatc cagaaatggt gtatacccat
    11341 ggccgtgatt aaagtatccg atatattcct ttgaaaagct ttctaataca ataacgcata
    11401 cattttcagg tttagatggc ttggcaattg aatcaggcca ctgataataa agtgcgttat
    11461 cgtgaaaaat attaaactgc tttgccaatt cctgttcatc ctcataataa acgggctcaa
    11521 ctagttgcct gtggccgaac gcgtaaatta cagagaatgg cgtattgata atcaaagatg
    11581 tgagtcttcc atcatcaaca taatctgttg caatttccgg tgaaataggt tccggttgta
    11641 taccacctct tgctccaatc acagaaaatg taagcaccac caccatgatg atgatctgat
    11701 atggataatg aataaaacgt ggtagtttga aatgcacttt tttaagtcga tgcaattctg
    11761 tttttcggta gagatattca actcctaaaa tcagtataaa ggcaatgaca aaaattatcc
    11821 agtaatcttt aattacctgc ggaagaatat ttgtatcacc tgtaagtccg agttcgtgcg
    11881 ttgatgttct ttttaatcca tattcataat agatccaatc gccactctcc aataataacc
    11941 caacgccatt aaaagaataa aaaatgatct tcaacatgcg ctgataccaa tttttatcga
    12001 accatggact tggaactaca tgcaatacta taaatatgca gttcacataa atgatactcg
    12061 acaagtcgaa acggagacca tacaacatca gctgcaaaaa ctctcccgga ggtatgtgcg
    12121 ggaaaagtgc catattaaac agaaagaaca gtatacggca aaccatatac aacagcagga
    12181 gaatgcccaa gcgtttgagc aatatctcaa tatggaacag ttgttctttc atacagcttg
    12241 caaagttact aaaacaaatt accgattatg ttggaagcgc cttagtgaat gagcgcttta
    12301 aaactcagat caaggctttt aacagagtgt gttaaggcgc ctactgaaat aaaatttacg
    12361 cctgtttcag ctatagcctg aacagttgtc aaattcacac ctcccgatgc ctcagtttcg
    12421 aactgaccgg ctacctcaat aaccgcctga cgcatagtgt ctacatcaaa attatcgaac
    12481 ataattctgt cgacatgacc tgttcgcaaa acctcagcaa cctcttcaag attgcgggtt
    12541 tcaacttcaa tgcggaggtt taaatcgttg tcgcgcaggt attctttaac cttcagaata
    12601 gcaggtgtta tcccgccaca aaaatcgatg tggttatcct ttataaggat catgtcatat
    12661 aaaccgtacc tatgattatg accaccgcca attcgcacag cccacttctc gaatgtgcgc
    12721 aatccgggcg ttgtttttct ggtatctaaa attttggcgc cggtatgtct aacggtttga
    12781 acaaagagat ttgtggtagt tgctattcca ctcatgcgct gcatgaaatt gagcatggta
    12841 cgttcggcaa ccaacaaggc ctggactctt cctgttgcat gaaaaacaac atcacccggt
    12901 tcaacgcgtg ctccgtccgg aatgaaaatg tcaacctcca attcggggtt gacatgtcta
    12961 agtatctgtt cagctaattc tacaccggcg atgatccccg gttcctttac aagcaatttt
    13021 gccgttccgg cagcctgatg atctatggtg gcgagggttg tatgatctcc ctcctggatg
    13081 tcttcaatta gggcctcatc tataaaatct ataatatccc aaatctcaac actactcatt
    13141 ttcaaagctt aactcctgga tatagaactt atcagcacgc tttttcatat acacgtaggt
    13201 tctgaattta cccttatcgg tagacagaga gccaatagca taatggccgt tgctgcctcc
    13261 tgaagtacct tcatgcaaaa agctgaaatc cttaggtgga tttttagtga aaaagtcttt
    13321 taccaccgct tcggcctggc ttttactgta agcgccttcc ttattcgcta ttttaacctc
    13381 gacagtatta tcaaagtaag tagcaagcga cttgctatca cctgatttaa ttgcagaagt
    13441 aattcccgca agctcgctct gcatagtagc gaaaatcagg aatggaacca ccgcaaggat
    13501 ggctataagc tttgttctgt tgttcttgaa catggctgta tttttggtca atgcaatggt
    13561 aaatgctaaa accataccaa aattagcatt ttttaacaag atacctttca acgtctgtgg
    13621 atgacatggc taaaatcaac ccaggtgcgg gtttcagctt tcaataactg cccgataaca
    13681 gttccttacc ctggaagttg caccaaaccg ccagaagttc aggttttagg agcattatcg
    13741 gcagtgacct ttcccatgct ataaaccaag cccagtatat acctttgcat catgaaatcg
    13801 atgaaaaagg tgatgctgat cattatggac ggctggggac atggtcaaaa tgcagccgct
    13861 agtgccattg cccaggctaa tacaccaaat gtaaacgcat attataaacg atacccaaat
    13921 gcagaacttc gaaccgacgg cgagtttgtg ggtttaccgg aaggccaaat gggaaatagt
    13981 gaagttggtc acctgaacct tggtgcaggt cgcattgttt atcaggaatt acagcgtatt
    14041 catatggcca tccgcaacgg tgaactggct tcaaacaata ccttattatc cgcactggat
    14101 tttgcaaaaa acaacggtaa gaaagtacat ttcattggac ttgtcagcga tggcggtgta
    14161 cattctcata gcaaccacct gatggcgctt tgtgatattg caaaatcaaa aggactcact
    14221 tccgaccaag ttttcattca cgcatttacc gacggtcgag attgtgaccc taaaagcggg
    14281 gtaggtttta tcagaacact gcaacaacat ttaacaaatt cagcaggcac catagcatca
    14341 gtatgtggac gatattatgc catggatcgt gataaacgat gggaacgcat taaaaaagca
    14401 tatgatcttt tagtgcatgg cgaaggcaat gctacgcagg atgttataaa aagtatcgaa
    14461 cagagttatt ctgataatgt aactgacgaa ttcattttgc ctattgtttg tactgacgca
    14521 caacaaaaac caatagctac aatagccgcc ggcgatgtgg ttatttgttt taatttcaga
    14581 accgatcgtt gccgacaaat tacaagagca ttaacacaag aagcgtttcc cgaattcgat
    14641 atgacaccaa ttgatctgca ttatgtcacc atgacacgtt acgatcatac atttaaaaat
    14701 gttggtgtta ttttcgaatc agatgatctc agcaaaacat taggagaaat tcttgaagcg
    14761 aacaataaaa cgcaaatacg tattgctgaa acagaaaaat atccgcatgt aacttttttc
    14821 tttagtggtg gcagagaaaa ggaatttagc ggtgagcgac gcattatgat cgcttcacca
    14881 aaagttgcga cttacgatct acaacctgag atgagtgctt acttggttaa agatgccatt
    14941 gttgaagcta ttaataacga aagccctgat tttatttgtt tgaattttgc gaatgccgat
    15001 atggttggac atacaggcgt gttttccgct gccattaaag cctgtgaagc tgttgatacc
    15061 tgcgtgggcg aggtaattga agctgcatta tcaaaaaatt atgccgttat tctaaccgca
    15121 gaccatggaa attcagatgt agagattaat gaagatggca ccccgaatac acagcacagc
    15181 actaatcctg ttccggtgtt ttatatcgat aaggagccgg cgtttcaaca aattagaaac
    15241 ggcaaactcg gggatattgc tccaacaatt ctaaccttaa tgggcattga cattccaaag
    15301 gaaatgacag gtgaaatact tgtttcctaa aacgactttc acaaaattaa aaaaggcctc
    15361 cgacatgcat atctcggagg ccttttatgt tatttcagca ttatttcagt tcactataat
    15421 atttaaagaa ccatggcaat gtttcaattc ctttgtaata attgaacaag ccataatgtt
    15481 cattcgggct atgtatggca tcgctgttca atccaaatcc catgagtatg gattttgcgc
    15541 ccaatacttt ttcgaacatg gcaacaattg gaatagaacc accttcacgc gtaggaatag
    15601 gttttttgcc gaaagttttt tccattgcca ttattgctgc ttgcatagga ggaaaatcca
    15661 gcggcacaac tacaggttct ccgccgtggt gatgggtcac tttcaccttc acatatggcg
    15721 gagctatttt attgaaatgc ttgtcaaata gttctgcaat tttttcagat gactgatcag
    15781 gaaccaggcg catagaaatt tttgcatagg cttttgacgg aagaacggtc ttacttcctt
    15841 caccggtata accgccccag attccgttta catctaaggt cggacggata gaactgcgct
    15901 ctaatgttga ataccccttt tcacctttga catcattaat ggcaagatcc tgtttgtact
    15961 catcgagact aaacggtgtt tttgccattt cagctctttc gctatctgaa atcaaagcca
    16021 catcatcata aaatccttca atggtgatat gaccatcttt atctatcaat gaagatatca
    16081 tatcacaaag tgtattgatt gggttagcta ctgcaccgcc atatactccg gaatgcaaat
    16141 cgcgattcgg tccggtaact tcaacttcca tataagtaag acctcgtaac ccaactgtaa
    16201 ctgaaggcac gtcattggcg atgatggaag tatctgaaat taaaattaca tcacaactca
    16261 ataaatcttt attttccttt atgaaagttc caagattcgg ggagccaact tcttcctcac
    16321 cttcaataat aaatttcaca ttacatggca gcgcattgtt ttgcatcata gcttcaaatg
    16381 ctttcacatg catatacact tggcctttat catcacaagc accgcgtgca taaatatttc
    16441 catttttaat gacaggttca aatggcggac tatcccaaag atcaacagga tctgcaggtt
    16501 gcacatcgta gtgaccgtaa cataaaactg ttggtaatga agggttgata attttctcag
    16561 cagtaactat cggatgtcct tcagtagcat ttacacgcac attgtcggca cccgcagcgc
    16621 gcaatgaatc tgcaactgca tgagcagtgc gggcaacatc agctttatat ttcggatcag
    16681 cgctaacgct gggaatgcgc aataaagcaa agagctcatc aaggagtctt tctttatttg
    16741 tttcaatata attattaata aaatccatat aatagtttta atgattcaaa attacttcaa
    16801 tttgacttaa gataccatgc ataatattgc acattgcctt tactgccgat aagcaccata
    16861 tgctcagatg ttatcagacc gggtgattcc ttcaaaacgg ttgcagccgg ttcaagattc
    16921 tgtttttctt ctatggatgg gtcatagatc acaagaatag gcttatcgtt taaatattca
    16981 tgcaaagtgc ctggactttg aggctcaagc agtaattgca tcatctggga agcaaaaagc
    17041 tgaggtgttc tggataattt aaccgacatg agcggcaagt ggctcttcaa ctgcaattgg
    17101 taagtatacc gggaccattc attggcatcg tcgatagtga aatcatattc ttcacagcca
    17161 acatgatagt atggaattgg caaaattgcc tggtaagcgt cataatccag atctggaagt
    17221 gatgcgagag atttttgatt aaagtaattg gggtaatacc ttgaattagt aaattgcttt
    17281 atcccataaa tatctgttgc aagcagggat aatccgatac acaatactgc attcactatc
    17341 catttctttt gattaaggat tattcgttca acggcgacca aaataccaat ttgagccgca
    17401 tagaacaacc accatcccat tctggcaata catcggaact ggctaacgcc cggataaatt
    17461 tttccgatat aatataaggg gttgaaaata ttctccatgg tgtttccacg cccaatcaaa
    17521 tgcaggtgca tgccggttcc ggcaaaaaat gccacaatgg cagtaagtat aaaaatccat
    17581 ccgaccggtg aaggtttcag cgttttaata aatgagacat ccctataccg gaaaaaacga
    17641 aatgcaatta aaataaatac catcaacaat aaataccaga agaatccgcc caaataaact
    17701 ccattttccg ggtcgaaatg aattttacct cctataaagg gaactaggtt cacttcatct
    17761 ctggctttat acaaatgcat aaaccgcatc tcccatgcac tgatattgct gccatccaat
    17821 tgagtaggtc gcattgagcg ccatggatca gtggtattga tcaataacat cataccaaaa
    17881 gttgcaatcg ctatggcaaa actcggaatt gcagtaaaat acagcacttc cgcagacttt
    17941 cttatttgct gaaggaatag tattcccaaa aaagcaattg caggtaaccc gagtaagatg
    18001 acataatata aatgtatccc tgaagcaaga aaaatcacga tggcgatcaa agctgcatgt
    18061 aagagcagtt tttttcgatt gcggctttgg aaattatcgt agatccgcaa taataacaac
    18121 atgacaagga aataaaccgg agtaagtgaa aggttataat tcccaacaaa aaatctcaaa
    18181 aattgagggc tcgtccaggt gattacaata gcacctattg ccacaagcca ttcattattc
    18241 aggaatctgc gaagtaccgg aataactgta agcggcgtaa gcaatattcc caataagaaa
    18301 atccattgga taattgctac agtatatggt gcgatattgc caaaatgcaa ttgaataaat
    18361 cgcaatggca tagcaatgaa aggcgaatta tcggtatacc acacagcatc accataagga
    18421 tattgcattg ctgtaaaatc aatatagcca tcagaagcag gttgctcaag gtaagcttgt
    18481 aaagtgaaga aattctttaa cccatcattc ctgccgctga ccataacatc gtttggtgaa
    18541 atgataaatc gccacatgaa gaagcaaaat aaagtcaagt gcgcgatgat aaaaacaccg
    18601 cgtatccgct tcggtgaatt ccaaaacgat tgattatgca tgggactgct ttttcgggtg
    18661 atgaaatcta taatgccatc catcccaacc ttgcaatcca cctgtatgta aaatcaatat
    18721 gcgttcggag gcggaaatgc ttttatttga aatcatatca tttacagcat aaaacacttt
    18781 cgcagtataa acaggatcgg gcttaatatt gtatttgtgt tcgaattgct caacaaatgc
    18841 cattaaggta tcattgtatt ttgcatatcc gccgaaatga tattggaaat taatatccca
    18901 ttgcgtttta ttgcccgcat caccgcaaag ttgcctgata tatggtgtta atgaatcttt
    18961 gtcggccttt aatgagctta taccggtaac tgttgcacta agtccgggac tacaaatgag
    19021 tccgcatagt gttccgccgg tgcctacagc acaaaatatt ctgtcatatt tatctgcaag
    19081 atcaccaagt atttcactgc agcctctgac ggcaaattca ttggtgcctc cttcaggcag
    19141 gacatatatt ttatttgcat caattgaaaa gtgatgtaaa aaatcactat tgtttctttg
    19201 gcggtaaagt tctcgagaaa cgaagatggt ctccatgccc atggagcgaa taaattttaa
    19261 tgttggattg aatggatcat cctcaccacg aagcactgca actcctcgaa ttccgtggtc
    19321 ataacaggca gttgctgcag catggatatg atttgaaaac accccaccaa aggttagcaa
    19381 agtgtcgcag ttttcttgtt taaagcgctc tatattatac ttaagcttat agaacttatt
    19441 tccctgtaaa cgcggttgtg ccagctccaa tctcagaata tcaaccggat ggtcatgcat
    19501 gtaaatcgta gttaccggag catgggtcat atgttaacaa agatgctgaa aattattatc
    19561 tttgaccccg cttccggggg tatagctcag ttggctagag cgcttgcatg gcatgcaaga
    19621 ggtcgtgagt tcgagtctca ctatctccac tttaaggtgt cctttgtggc accttttttt
    19681 atttcccttc ggctaaaagc ttcatcattt catcggcagt tcttgcgctt gctcctactc
    19741 cacccaatac ggtatataat ttatcatatt cgctcaacat agtattgcga tatgccgcat
    19801 ccttaagtaa gagatcgaga tgagtgcgca gtgaatcgtg attgaaatca tgttgaatta
    19861 attcaggcac cacttgttta tcagctatca aattaaccag actgatatat ttaattttca
    19921 ccaggcgctt tgcaatatga tatgatatga cactcccctt gtaacaaaca acttgaggca
    19981 cccggaaaag tgcagtctct aaagttgccg tgcctgaagt taccatagct gcataagcat
    20041 ttcgcaaaat atcataagta ctacctgaaa tgtaaatgat attctcccgt ttttcgaagc
    20101 cggcataaaa actatcgtcc attcccggag cgcaggcaac cacaaaaaca aattccggat
    20161 aatactttgc catactgagc ataaccggca atttaactgt aatctcttgc tttctgcttc
    20221 cgggcaatac agcaatcaat tgtttatcgc ctaactgaaa ctgttcacga aaatgttcat
    20281 tgcctttgtt tgaagcaata gcatcgagca gaggatggcc gacaaaggaa accggataat
    20341 taaatttttg ataaaatgct tcttcaaacg gaagaataac aaaaagtcga tctacatatt
    20401 gtttaataat attcacccta ctacttttcc acgcccaaag ctgcggtgaa atgtaataaa
    20461 acactttaat tccaatttca tgtacatggg gtgctatcct caaattaaat cccggataat
    20521 cgattagaat cactacatca ggtttccacc cggttatatc ctgtttacat gatttaatat
    20581 tattcagaat ttttctgagg tgcataaaca cttcaacaaa tcccatgtaa gcagtttcct
    20641 tataatgtgt tacaatatcc actcctgctt cgtgcatagc atcaccgcca aatccgcgga
    20701 aaactgcatc aggatccttg gccgataatg cgcgaaccaa attagcgccg tgtaggtcgc
    20761 cagaagcttc gccggctatg agatagtagc gcaagatgaa ggttattgaa aaattgttga
    20821 atgatgctga atataaaaat acactaggat gaacattgca ctcaaaactg taatgatacc
    20881 aacacctcgc atacaattat cctttcgaac acgcatataa atgacaaaag gtaaaatatt
    20941 aaaaaagaca gccagcgttg caatgaatct ttcagtcact ccggtaaata ttgcaccctt
    21001 aatggtaata tttccggata gactctcatt cagttgaaaa aaggcataat aagttgtcac
    21061 cataaaaaaa acagcaacag ccattcctac gaaaacgtta tcttcctgta gtttgctcat
    21121 atcaattatc cgaggtcatt aaacgtatca gagttatcta agtgtatatc gtgaggtgat
    21181 tttgtttgat agagtgacca gttcttatga aaaaacgcaa tcgcctggtg tgcagtaagg
    21241 tcatattgca cgggtacgat ggacacatag ccatgcgaca atgcccattc atcggtgtca
    21301 tcgccaccat cataattcac aaaattacct gttagccagt aataaggtcg accatgcgga
    21361 tcgaggcgtt catcgtattc ttcctcccat ttcgcatgtg cctgcctgca aatgcgcatg
    21421 ccatttattt cgtcgatggg cagatatgga atatttacat tgagtaacgt gttttgtggc
    21481 agtccatgtt gcaatacttg ttctgcaatt tttgctgcgt agtgcgccgc tgaggtaaaa
    21541 tctgcatcaa ttgcataatt caataaagaa aatccaactg caggaatccc ttctattccg
    21601 gcttccatag ctgcggacat agtgccggaa taaataatat ttattgaaga attacttcca
    21661 tgattgattc cggaaacgca gagctgtggt tttttatgga gtattttatc cttcgccagc
    21721 ttaacacaat ctaccggtgt tcctgtgcat gcatacgctt cgatatcacc gaaaagattt
    21781 gattttttca atcgcaatgg ctcattgagg gtgatggcat gtcccatacc gctttgaggc
    21841 ttatctggcg caacaacaac aatggttcca agtggacgca ttgcttctac cagcgcatgt
    21901 ataccgggag catgtatgcc atcatcgtta gtgatgagaa taatcggctt attgttgttt
    21961 tgctgcatga tactattgtg ttgacggaaa ataataaccg acaacaaaat tactgcaaat
    22021 aggttggtaa cacgaattat tcgttgcctc cgatgagcgg tgtgagttca acagggagta
    22081 caccgttgct ggagcgcaga ccacccaagt tgttaatcac tttatccaaa tacccttcct
    22141 tcgaaatttt gatagaggta gatactccgg aaagcgtgct cacatcgcca tatccgtttg
    22201 gatctgtttc acagattact tgtgaattag gactttcaaa gaaggtaacg cgcgctttat
    22261 aaatggcatt tttattattg tcgtaaacaa atactgaagc gatctgagcc atttgggtta
    22321 cttgataaat atcatcgccg cccatgccac cgggtctgtt ggaactaaaa taaccaacgc
    22381 catttttcat ggtaaagcca taatcatcct gaggtgaatt aaccggcatg ccgaggtttt
    22441 cgggagttga ccaatgatca ccttcgcgtt cgcatttaaa taaatcgaat ccgccaaatc
    22501 ccggatgata attactcgca aaatacaaag tgccatcggc tgtcatgaag gggtacaatt
    22561 catctccggc agtattaatc acatcaccca tgcttttggg ttctgaccaa ttgccattgc
    22621 gcttcataca ataataaaga tccgttcctc cttttccgcc ttgcttgtct gatgcaaaaa
    22681 ttaaaatgtt gccatcggga gttgttgccg gatgacctac tgaatatatt tttgaattga
    22741 cgttcaacgg atgaggcgtg ccgaatttac tgtctacata aggtgcataa aataactcca
    22801 gtctaacctg cccatcgatg ctcaccactg ttctgttttt ataatggttg ctgcgcgtaa
    22861 aatacaaacc tttagaagca ccatcgtaat aaggtgcagc atcatgataa ggtgagttca
    22921 catgatcgag tttatttgta atccagcttc cgtctgttcg cacatcggct gaatataaat
    22981 cataataagg cagacctgta ttgttattga tttttgtgcc attactctta gcaaaaataa
    23041 ttccgttctt ataaatggtc ggtgtcattt ccgagccttt ggtattaaat ggcaatgtat
    23101 cgatataata ttgcgatggc ttattcatat ttgccaatgc ccaatcgcat gttgatgcga
    23161 gataatatcc atcaggttta taagccgcat attttaaaaa atatgttttt gccgacctgt
    23221 atttgccgtt cattttaagc agcgtagcat atctaagtaa tgtaccggca tcagctatat
    23281 tatgtgatac gataacgcca taccaatgct cggcttcgct ccattcctcc attcgataat
    23341 agcaatctgc caatttcaca ttgacgatat aatcatattg ctgctccaga tggtcgatat
    23401 agagtgctgc cgcttccgga taaattccat tattgtaata tttatccgct tttgccagaa
    23461 tttcattctg tgcgtgcagt gggctgattc ctgaaaaaag gattatgatc agacagtaac
    23521 gatatatagc ttgcatgcgc atcagaaaaa tcttggatta acaaacttat tgttatgctt
    23581 ataaaaacga taggtcagga acacctcatg agatgggctg ataaatacct tgtcatctgc
    23641 caacatcgta ttgtaagaat atcctaagcc ggcttcatgt ccggtgatct tattatctgt
    23701 gctgaaaata tattgaccca caaatgagat acttccattt gttcggtaac caccacctac
    23761 caataaaacg ttgttatata taaaatttaa attcacatcc atttgagcag gaatactatc
    23821 agtaattttg atcagcaagt ccggctttat ggtaagctta tcattaatct ttatcagtgt
    23881 tccaagtgta ccatatacag ttgcaacctt gtcgaatttt tcatcttgag ctacatcagg
    23941 gttgtcattt ccattagcac ttaatccgga tagagacatt acagataatc ctacataata
    24001 aatatcggaa tgcaggtaaa ctccaagacc ggcatcaggc gaccaataag actcagaagc
    24061 cttagtaaaa gtggggtcgc cattaataaa ggcatctagc tggctataat caactttgta
    24121 gttagcgatt gaaccttcta tcccgatagc tgctgtcagg tgatcgctga tatgcatgcg
    24181 ataggcgtaa tcaagtccca caaaactatt attagtaaca cctagtttat cgtgatagat
    24241 tttcagtcca agtccaacat tatcatttgc gagcggagta tgcaatgtga atgattcggt
    24301 ccccggagcg ccttgaacat tggtgctatg tcggtgtaaa aagaatccgg cctctatacc
    24361 gcctttgcta cctgcatatg caggattgaa taccaactga ttcaaatgat attgctcaaa
    24421 taaaggcggt aactgcgcat acaatgcatt tgcactgcat aacatcagca ataggagtgt
    24481 gctttggtat aatttcttca tcgtttgact acaataaagc cggataatgg atcaggcagt
    24541 ccttctatac gaatcacata aaaataagtg ccctcaggaa cgttacctga tgacgaaata
    24601 aattccacat ttgacgtgcc atcccataca tctgaggtgg caagcttttc tttctcaaat
    24661 acaagactgc tgttgcggtc atatatattc acgttgtatt taattcccga ttcaagtcct
    24721 tcgatggtga gcatatcatt aataccatca ccatttggcg agaaggagtt cggcacatga
    24781 attgcttctg cgggaatttc cggctcttca attttcatat gcacatatgc agtatcgcag
    24841 tatggaggac aattattgtc gcaaacaaca tatctgaaat catcggtgcc aacaacaaaa
    24901 ggaatatatt tgatggtatt atctgcatta attgttgctg ttccggtcat gccgtcagca
    24961 atgatctctg aaacgatcat agcatcgcca tcaccatcaa aatcgtttaa taaaacaggt
    25021 atatcaatag tatcgataac tactactgtt gctgtgtcat ttaatgcaga aggtggattg
    25081 aaatttgtag tttgcacatc tacacataca gttgctgtat cgcaataaat atcggaacct
    25141 attttacaaa tcttgtaaat gaagcaatcc tctccataca cacaagggaa aggttcataa
    25201 aaaacagctt caccgaaaat gatggttgtt gcaccataat ctgcatcgtc gataatggta
    25261 aattcaaggc cttcgttatc tacatcaata tcatttagta atggttcgat attgagcggt
    25321 gtatttacat aagtttcaac tgcatcatca acagcgattg gtttatcggg aacagggttg
    25381 atagtaatga ttacacttgt tgttccacaa atgtcgggat ctgtattagc acatccggta
    25441 tattcgaaat tgtcggtgcc atagtagtcg tcattaggtg aatagatcag caggtcgttt
    25501 actgcgtcaa ccgtaacggt tccatgcaat ggcaaagttg ctatgttcag gtatacatcc
    25561 gtgacaccgg tagtattgag atcgtttgcc ataacgtcca tggtaacatc aggaacatct
    25621 tcgttgatct cgtaaaaatc aggggctgca tcatatgatt gagcatgtac catagaggaa
    25681 gaacccaata tggaaaacaa tatgcttgtt atgatccctg cgtagcgctg catcatcagg
    25741 taaaaataga gatttattct tcatttaaaa acgagtaacg gaaatccacc ggtggaacaa
    25801 agttctcctt gatggttctt ggcgacaccc atcgcaacag attcaggatg cttcctgcct
    25861 tatcgttggt tcccgaagcc cttgcaccgc caaatggctg ctgacctaca actgcacctg
    25921 ttggcttgtc attaatgtag aagtttccgg cagcatggcg aagagcgacg gtagcctcga
    25981 ctacagcata tctatcttgt gaaaaaatag ctcctgtcag ggcataaggt gaagtgttat
    26041 tcactacctg caaggtttcc gaccactttt cggcatcgta tacatgtatt gtcagcaccg
    26101 gtccgaagat ctcctcgcac atggttctgt acgacgggtc tttagccaaa ataacggttg
    26161 gtgctataaa ataacccttt gatttatcat aagttccgcc ggcaattatc tcctgaccat
    26221 cggcttttgc tttggcaata taaccgctta tggagtcaaa tgccttttcg tcgataacag
    26281 catttatgaa gttcgaaaag tcctcaatcc cgcccatttt catttcagca agatcagtga
    26341 caaggtaacg cttcacttct tcccacatat ttgaaggaat ataggcgcga gatgctgctg
    26401 aacatttctg tccctgaaac tcaaaagcac cgcggctcaa ggctgttgcc acttgttttg
    26461 gatgggcgct tggatgtgcg attacgaaat ccttaccacc ggtttcgccc acaatgcgcg
    26521 gatatgtttt gtaaacatga atattgtcgc caatggtctt ccaaatggtg cggaaaacct
    26581 ccgtactgcc tgtaaagtgg ataccggcga agtcaggatg gctaaaaatc acctttccgg
    26641 cttcacttcc gcttacgtga acaaggttga taacgccgtc gggaagtccg gcttcacgaa
    26701 gtatttccat gatcacccag gcagaatata tctgcgtatt ggaggtcttc caaacaacgg
    26761 tattgcccat catggcaggc gctgttggca ggtttccgcc tattgaagtg aagttaaaag
    26821 gtgaaagtgc gaaaacaaat ccttccaaag ccctatgttc catgcgattc cagataccgg
    26881 gagcactttc cggttgatcg gaatacactt gctgcatgaa ctcggcatta aatcgccaga
    26941 aatcaatcag ttcacaggca gaatcgatct ccgcctgaaa tggattctta ctctgtccga
    27001 gcatggtggc agcattcatc ttgtatcgat aaggtcctgc cagcaactca gcagctttca
    27061 ggaaaatggc tgcacggtct tcccatggcg tattttccca tgcttgcttt gccgtcaatg
    27121 ccgcgtcgat agccattttt acatgctctg ccccaccctg atggtaatgg ccaagcgtat
    27181 gggcaatttc atgcggagga tgaattgcta ccgtttttcc ggtgcgcact tcctttccgc
    27241 caataaacat gggtatatct acagaaatag ccttaaggtc cgcaattgcc tgctttaagg
    27301 ctttgcgttc aggactgccg ggagcgtagc cttttaccgg ttcattcagg gctttaggta
    27361 tgcgaaaaat agcgttagac atagtaattt caactttgct gcaaagatag ggctttagcc
    27421 atggaaatcg actgttgata aacactttta ggttggtaaa cggcactact tctatcaaaa
    27481 ctcagacatt gctcaaatgc agattttaca gcacctttgt ataaataggc gcgctttttg
    27541 catataaagg cggtttatta cttaattcac aggcatgcat aaactttcta cctacagttt
    27601 cggtattgta ttttccttat tgctgttatt actcccttca tgggcatcgg cacaatcgcg
    27661 cacgcagcaa ttcaatcagt tgctggacaa aaacgatact gccagagcgc aggtgctcct
    27721 tacgcaatgg aaaaaagatg tgcccggtga tccggaatgg tatgttgcca ggtacaattt
    27781 tcttgtttgg agcagcatga agagtattgt taccataggc agcaatccga agggcaataa
    27841 ttacatggag ataacaccaa cggatacctc tgcagatgcc acgccgcaat tcatgtatgc
    27901 cgatgtctat tatcagccaa aacaagtagc atctgctatt gcatgcatag attctgcgat
    27961 tgcattattc cccgataggc tcgacctgcg atttggtaaa atctatttat tgggagaaac
    28021 tcaatcttat caggcatttg ctgatgaaat agttaaaacc atccattacg gggcaacatt
    28081 taatcataac tggaaatgga ccgatggttt agcaattgat ggaggaattg cctcatttaa
    28141 tgaatcgata caatcatata ttgttcaatt gtatgatact aacgtagata gccttggcgt
    28201 gcaaattgga caaatcgcaa cagcggtatt acaatacgat ccaaacaata tcgaaaactt
    28261 gtcaaatctt gctattagtt atatgctggc gagtaactat gaggaagcct tgacgccact
    28321 gcttaaagct gaaactgttg atccgacgga tgccatagta ttgagtaata tcgcattttg
    28381 ctatcatcgc atcaacaata aggaacaggc gattgcttat tacgaaaaat caatcaaata
    28441 cggcgatagg gaaatgaaag cgttttccta tgaacaaata caattaatta attcggaaaa
    28501 atagtatgga ttgggtcatt gaaactccaa gattattgct gcgcgaatta aataccgacg
    28561 acgcacaatt tttctttgac ttaaataatg atcccgaagt gattagatac acaggcgatg
    28621 ttgcatttga aagtatagat gcagccaggc aatttttggc cggatatgat cacttcaaaa
    28681 aatatggttt tggtcgctgg gcaatggcga caaaatcaga gcacaagctt gttggctggt
    28741 gtggattgaa atattcctct gatgcgaatg aatatgacct cggatttcgg ctatttaaga
    28801 atgaatgggg aaaagatttt gccacggaag cgggaattgc atgtttaaca gcaggctttc
    28861 aaaaatttaa tatgccgatg atcgtcggtc gtgcgttgaa agcgaatata ggctctatca
    28921 gagttttgga aaaatgtggt atgcgttact ggaaagatgt tgaagagcac gataatgagg
    28981 ccgtggttta cagatgcttc cctgaagatt taaataattt attgtgatat catttaaaga
    29041 aataaatcaa tatgcgtaaa ctcatcagtt ttttatcctg tatttttctt gccggaatca
    29101 tgcacgcaca ggtgacaatt attgacagca tttatagtgg aggcatgtac cgcaattacc
    29161 gcatgtatat accaacagca tatgatggat ctgaggcggt gcccttatta tttaatttac
    29221 atggttacag ttccgacaat ttcggtcagg cattgtatgc aaattttaat ccaattgccg
    29281 acacagcggg ttttatcact gtgcttccga atggcacttt tgatgcgttt gggttacgct
    29341 attggaattg ttttgttgca cccggcattg gtgtcgacga tgtgcaattt ttatcagacc
    29401 ttatagattc tgttgccgca gaatataata tcgacctcaa tagagtctat tctacaggaa
    29461 tgagcaacgg tggtttcatg agttatacgt tggcatgtga attaagcgag cgcatagcgg
    29521 caatagccag cgtggcagga actattgaca aagacagact ctcctattgc aatgcgcaac
    29581 acaatgttcc tgttttacat tttcatggca ccgctgatcc gacggtgccc tacggcgaca
    29641 accttgcatt tttttcggtg gattcagtaa ttaattactg ggtagcgcat aatcatacgg
    29701 atgccacacc tgtattcaca gaagtaccgg acattgtgac aacggatggc agcactgccg
    29761 aacattatgt ttatggcaat ggctggacag acagcagagt agaattatac aagattattg
    29821 gtggtgaaca cacgtggccg ggcacagcaa tcacctttct tggaattacc aatcaggatg
    29881 tgaatgcctg taaagagatc tggcgttttt taagtcaata tcgacttgat gaattaagtt
    29941 ctgttgaccc tttaacagac aatgaccttg ttcaaataaa cccgaatccc gctgatcaat
    30001 ttcttgttat taattccaaa ttttcaatca atggtgttga aataattaac atgaatggtc
    30061 aaataatcaa acaattcaat cctgccggcg caactcaagt gaacatacca ctttcagaat
    30121 tagcgcaagg tttatatttg atcaaaatca gcacaggcga tacttacatc acccaaagat
    30181 ttatcaaaca ataatatttt aaccaggtta tttttgtgcg cgattacttt gttttgcgca
    30241 tgcacaccat attgtagcta tccacctttt gcacattata ccaagtgcca ttataagtgt
    30301 acaatgaaac agcttgtgtt cctgcagctt cggtagaagt ccagagtgtg tagctgtctg
    30361 accaattaaa tttatcgata ttcaaataaa tcaaggctgc ttcatctttg gcaggcagat
    30421 accaatcgtc atgaccgccg gctgtgctat tctcacaaac ttgagccgca tatggtttat
    30481 tgtcaaaccc gggattatcg cctaccgcaa caacaatagc ttttgaattt aatgccccat
    30541 ccgtttcact gcgagtagcc acaccaatct cttcataacc accccaagta atttttgctt
    30601 ctttagtaag atcgagcact tcataacttt gttcagcgct gctgattgga acaatagttt
    30661 gagcttgagc ctgaacaatt aagccgcaag tcaatatggt cagaaagatt ttcatttgat
    30721 acggatttga attgggttaa ataaagggtt gtctactaaa gatataacaa aatattaaat
    30781 ttcgacatgc agtcatttat atgaaaccca gtttgtgatt taattcgcta cttaggtggg
    30841 gcaatttatt ccgctggatc aggaagctgc tcagcacagc aaaatcttta aaaatataat
    30901 gcttatttaa tgctccttgt aagtattctt ttcctgagct gccgcccgtg ccaactctac
    30961 ttcctatcat acggtgcacc atattgatat gacggaatcg ccatgtagcc atttgttcat
    31021 caatatccaa caaatgttgc atcaattgaa aaggtaaatg catgagtgga tatccgcgat
    31081 aaagcgaaat gaacaaggca ttgcgcatag ccttattact tagagaagta tgttcacttt
    31141 tcccttcgcc caaaaaaata gaagcaaata tctctttgtt atgttgttct ccttcaacca
    31201 aggttttgct ataggcattc agataatcat tccaaaatac gtgatgcgtg ctgtcatttt
    31261 gtgatgcagg ctgataactc ttccaaagat tgtcgtcact aaagaaaggc atacgtgcca
    31321 gccaatcgtc gagcatttcg agcagtgatt tttgtgattc cacatctttc accaaatcga
    31381 catattgcgg ctttagcatg cttgtataat attctttccc atagcggtta tcaaacttca
    31441 aacccagttt cgcctcaagc attttgaatt gccaactttg aaatccggag gcggggcgaa
    31501 gaaaatcgcg gaactcatga aaatcgagag gcgtcattgt ttccagaata tcgatctggt
    31561 gcaccaaaac ttttaaaatg gtcactaccc tatcgagtcg atgtacaaca gtttgtaatt
    31621 cgggactatt gtcatttact tcgggtttac ccatcacctg cagcgcgcta tctacttcaa
    31681 aatgcacctg tttaaaccac aattcatagg cctgatgaat gatgataaac agcatttcat
    31741 catgtgcatg ggctccggcc ttatcgcttt caaggctttg agcattcagt attttatcta
    31801 actggagata ttcggtatat tctacgggct tatgtgacat ttcctgctat ttgcgtcaaa
    31861 aatacataat gcggggtgaa tgaccgatga cactgctcaa accctatcca tggacagata
    31921 tcgatgacta aaataagata cctccacctt tggttctgac ggatggccaa ttttgaaact
    31981 acgaccgcac tatccaaaat cctagtcatt ttcagaccaa cttcgtgtat aaacgcgttc
    32041 ttaagggagg tggatatcaa tacctttact tttgagaaaa actcatttca aaaatagcat
    32101 cgtgtttttt gcacaagcat ttgcccttta accaagcccc cctgaagcag caaatacctg
    32161 aaattgtaat acaattcggg taggaaagaa aaacaggaat gggacagaaa agacaacaaa
    32221 tgcaattgcg agctgagttc aaagatcata gcaaattttc acatggcgga aacaaagaaa
    32281 tcaggtaaga aagcaaagaa agcatcaaac agcgctccat tttacaaaga tgagcgcata
    32341 ttgaaggtta cgggagtatt tttattactg ctgagttttt tcagcggcgt atcgtttgtc
    32401 agctatctgt ttacctggaa ggctgatcag agtcttatct gggatcatcc ctgggcgcaa
    32461 gttttcaata gcggaattac agtagaaaat catctcggca atcttggagc agtattgagt
    32521 catcaattct ttttttgctg gtttggtgta gccagtttct ttttcgtggt ttggtttttt
    32581 acactgggag taaatttctt tttcagccga cagatattca acaatgtaaa actcataggg
    32641 cagggaatgg tagtaatggc atggatcagc accatgctcg gttttttcat gtataaagcg
    32701 gaatttcctt atggcggtgg attcggcaaa gggacagcat ttcatttgga gagttatatc
    32761 ggcaaagtag gcgccatttt agcacttacg gcggcgatgg tagtatacat gatcatcttc
    32821 acaaaatttg atttcggatt tttattgaat atgttcagga agaaagaagt aattcctgcc
    32881 gtagatcctt ttacggaaga tgatatgcct gttcaaacgg aggaaatccc tgaggtggaa
    32941 aaagaaatga tcaatgttcc tcccgtttat acatctaacg tagatattac accgttacag
    33001 ggagaggttg atgatgtggt aatggaattg aaaaaggttg agccggtatc ggaacaatct
    33061 atgcctttgg aaattgagga gcccgaaaca attgttattg caggcgatga agaacctcta
    33121 ctagatcatc aggatcattt atcactcgat attgaaacac cggtggagga gcagattgca
    33181 gcggaaaatc ttaattacca tttcgaccct gttctcgatc ttccaaatta taaatatcct
    33241 gaattagatt tattggaaga atatgccagt gaaacggttt ccattaatac agaagagctc
    33301 gaaagaaata aagatcagat tattgctaca ctgaataatt ataatattca gatcagcaaa
    33361 ataaaagcaa cgataggacc aacggtaaca ctgtatgaga tcattccggc tgcaggtgtg
    33421 cgtatcagca aaatcaagaa tctggaagat gatattgcat taagtctggc agcgcttggc
    33481 attcgaatca tcgctccaat cccgggaaaa ggtaccatcg gtatagaagt gccgaatgtg
    33541 aataaagaaa cagtttcgat gaagagccag ataggcagtg ataaatttca gcatgcgaaa
    33601 atggatcttc cggtatgttt gggtaaaacc atcagcagcg agaactacat tgccgatctt
    33661 acacgcatgc cgcacttgtt gatggcgggt gctaccggtc agggaaaatc tgttggtatc
    33721 aatgccatac tcatttcact gttatataaa aaacacccat cgcaactcaa gtttgtgatg
    33781 gtggatccca aaaaggtgga attgagtttg ttttcatcca tagaaaagca ctttttagct
    33841 aaacttcccg gagaggaaga cgccattatc accgataccc gtaaggtggt aaacacgcta
    33901 aatgccttgt gtattgaaat ggatacccgt tacgagttgt tgaaaaatgc gcaagtccgc
    33961 aatatcaaag aatacaatgc caagttcatg aagcgcaagc tcaatccgaa ggaagggcat
    34021 atgtttctcc cctatattgt attggtgatt gatgagtttg ccgatcttat aatgacggca
    34081 ggaaaagagg tggagatgcc tattgcgcgt atagcccagc ttgctcgtgc ggtgggattg
    34141 cattgcgtga tcgccaccca gcgtccatcg gtgaacatca ttaccggtac tatcaaggcc
    34201 aacttcccgg cgcgtatcgc tttcaaagtg gcgagtaagg tcgacagcag gaccatcctt
    34261 gacaatagtg gagccgaaca gctggtagga cgtggagaca tgttgttggc gattggaagc
    34321 gatatggtgc gtttgcagtg tccgttcgtt gatacccctg aggttgacaa ggtggtagac
    34381 ttcatttccg accagatcgg atatccggag gcattcatgt tgccggagtt cattgatgag
    34441 caggcccaga aggaaaaaat ggagattatg gacggcgagc gcgacccgtt gttcgccgaa
    34501 gctgcaaaaa ttattgtgca acaccaacag ggatcaacct cgcttattca acgtcgcctc
    34561 aagctaggat acaacagggc cgggcgtttg atggatcagc tggaggcagc cggcattgtt
    34621 ggtcagagtg ccggaagcaa gccgagagaa gttttataca gcgatttggc acaattggaa
    34681 cagtatttgc aaaccttgga ttaaacttaa taatccattg ttttcaaatt aactttgcac
    34741 ttccaatcca cactgtcatg aaaaagatca gtattatggt gtttgccctt gtgatgaccg
    34801 cgggtatcgc ccatgctcaa acctccgacc cagcagccgt taaattgctg aaatccgtta
    34861 gtcagaaata tagtgggtat aaaaccatgc aaatggatat tgcccttacc atcgaaaacc
    34921 ttgaggctaa aacaaaagaa actaaaaacg gtaaagtttc tgctaaagga aacatgttca
    34981 aggctgaaat gggaaatcag atcattatgt ctgatggtaa aacactttgg acctatctaa
    35041 aggatgtaaa tgaggttcag atcaataatt ttgaaaaagg agccggtatt atgactccta
    35101 acgacatctt taaaattgct gaaaaagatt accttgctta tatgggtgaa aaggtgaccg
    35161 aaggtggtaa atctttgcaa ataattgagc ttacaccaaa agacaaaaca ttgagcttta
    35221 gcaaagtaaa aatgtatgtt gatccaagcg acaattcgat taaaaaaggt gttgtattcg
    35281 acaagaatgc gatacattat acatacgtga tcagcaattt caaatcaaat gttgaaatga
    35341 acgataccaa cttcaaattc gacaaatcga aatatccagg ttgtgaagtc attgatttaa
    35401 gagaataaac tttttagaca tattgaaaag gtcgtctgca cacaggcggc ctttttttat
    35461 gcatattaaa ttgtggttga gcggttcaaa tgttttctgc aaacaaaaaa ggctgccatg
    35521 acagacagcc tttctgaaat agcgtagagg ccaattatct tataagcgtg atattacctg
    35581 catattgcgt agtgataccg ttttcaaatg tgagctccat aatataggca taaaccccat
    35641 tgttcaaaga ttcgccatta aatgtgccgt cccatccttc agtatcgttt gtagcaggga
    35701 tattgtcttt ttcaaaaatc agcgcacccc atctatcata aatgcgcata cttctgatag
    35761 aggcgagatt gtaaccatag gcgaaaaaat aatcattgaa attatcgcca ttcggtgtaa
    35821 atgtatttgg aatataagat gcattgccag gatttggaag cacctcaata tatacatcgt
    35881 cactagctat acatccgttc tcattagttg ataccatggt atataaaatc gactcgagcg
    35941 gatacgcatt tgtttcggca caccatgtgc aaataacttg tgtaggaggt gtccagttat
    36001 aatcgccgcc tgcttcggaa tgcagcactg caggctctcc ggcataaata gtggtatcgc
    36061 cccatgccgt ggcggtattt tcatctacac taatagaaac ggttgctgaa gaagagcaac
    36121 cataaacatc ggtataatcg tacgttatgg catacgggcc accaacaccg gctgtagcag
    36181 gatcgaagga agtgcctgtt gtccctgttc cggaataagt gccgcctgaa ggataagcat
    36241 ttagcgtaag agccgcatct tcaacacata caacatcatc aatggtaaaa attataaccg
    36301 gcagaggatg cacagtaaga tttgtagtaa tgattgaatc acatcctgct actgttgaat
    36361 atgaggtaac gtatgttccg gatgcattta caatattacc atcaggtaac gtatacgttt
    36421 cgccttcaca aatttcaggg ttgaaggtag ggaaataagt aggatcaaca tttaatgttg
    36481 ttgttatgat ggaatcacat cctttatcag tcagcaagtt tgcaatatac acgcccgcta
    36541 cacctgtttc aattccatca ggcaaaatat aagtatctcc ggaacagata ttaactgttg
    36601 atgttaaatc gtaagttgga aatacatata aataagtagt gattaacgaa tcacatccaa
    36661 atgctgtggc gaacattgtc gggtaaatac cggcaatatt tgttacagtg ccatcaggaa
    36721 gtgtataggt atcatcatca caaattgata cataaacgtt gtcattaaaa tttggatgaa
    36781 cggca
//
LOCUS       DAOQHY010000009        36050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_470_length_36050_cov_5.884317, whole genome shotgun sequence.
ACCESSION   DAOQHY010000009 DAOQHY010000000
VERSION     DAOQHY010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 36050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36050
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_470_length_36050_cov_5.884317"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..728)
                     /locus_tag="PLL28_01550"
     CDS             complement(15..728)
                     /locus_tag="PLL28_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNF68025.1"
                     /translation="MKKIIHTAISRGHANHGWLDTYHTFSFGHYYDPDRVHFGALRVL
                     NDDTVSGGMGFGKHPHDNMEIISIPLEGDLRHQDSMGNVAVIKQGDIQVMSAGTGIFH
                     SEQNAHADKQVKFLQIWVIPNKQQVAPRYDQITLDPAAGINTLTQIVSPNADDEGVWI
                     HQNAWFHIGTFDKGTTTNYTQHDATNGTYFFVLEGSFEINGEKLDRRDGMGVTELSAH
                     QIKALSEGSRILIMEVEMI"
     gene            903..>1705
                     /locus_tag="PLL28_01555"
     CDS             903..>1705
                     /locus_tag="PLL28_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68026.1"
                     /translation="MKGLIPALLVGLFSTALQGQILTGVTLEGEIYPAHEDLITDPGF
                     FILDCDNDSLGALPLVDLGTGYYMGYQGGLFPGGSNNDPNPHFNNGKNISKAIKPLND
                     LGLVDYLTGEVSLIGIGPSVASDPYNEWKETQNDIDWPGVNPCLNVKGNFIGGKSVAD
                     MLDIDGTYWASFLTGLASKNIDPLQVQIAWMLLVSSTDSNDVNYYVDTVTNQYIQVLH
                     NLQTLCPNLQQVFISGIHYTGYTSPEHKRYDYLIEPYGYWSNLVVKNVIT"
     assembly_gap    1706..1805
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1806..2024
                     /locus_tag="PLL28_01560"
     CDS             <1806..2024
                     /locus_tag="PLL28_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68027.1"
                     /translation="AHDVIQIPVDENLQGESTLMMYDQYGSLLCVQHFDARTTLTCEV
                     QINNLPSGIYYVLMRDHKQTIAGRFLKT"
     gene            2595..5888
                     /locus_tag="PLL28_01565"
     CDS             2595..5888
                     /locus_tag="PLL28_01565"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HNF68028.1"
                     /translation="MASKKKPTTGGLSFERYFTQDGVSPYDQFTYELRSSVIRNTTGD
                     AVFEMHNVEVPQDWSQVATDILAQKYFRKAGVPQADGSTGSETSIKQVAHRLADCWRQ
                     WGEQYGYFASSKDGQIFYEELAYMILAQMGAPNSPQWFNTGLFSAYGIKGKPQGHYYV
                     DPDSGELKKSTSAYERPQPHACFILSVDDDLVNQGGIMDLWTREARIFKYGSGVGTNY
                     SKIRGEGEKLSGGGTSSGLLSFLKIGDAAAGAIKSGGTTRRAAKMVCLDLDHPEIESF
                     VEWKVKEEDKVAALIAAGYSSDYEGEAYRTVAGQNSNNSVRIPNSFFKVLEEEGQWEL
                     IGRTNGKVFKTVSARKLWDKIAYAAWRCADPGVQYDTTINEWHTCPKSGRINASNPCS
                     EYMFLDNTACNLASLNLRKFYDDDKLVFNVDAFEHACRVWTVVLEISVLMAQFPSPEV
                     AQLSYEFRTLGLGFANLGSLLMVSGIPYDSDKARSIAGAISAIMTGISYKTSAEMAGA
                     LGAFKGYELNKEDMMRVMRNHRYAAYNAADNYEGLEIKPMGIDQALCPEYLLKAACDA
                     WDEALQLGDKFGYRKAQATVIAPTGTIGLLMDCDTTGVEPDFALVKFKKLSGGGYFKI
                     INQSIPLALKNLGYKDESIESIVNYAKGTGTLKGAPSINYDTLKQKGFTETEIDHVDK
                     VMASAFEISFAFNVFTLGAETLERLGFSAEHYSEPGFNLLRALGFTREEIEAANDHIC
                     GTMTIEGAPYLQEKHYSVFDCANKCGKIGERYIHQLGHVRMMGAVQPFISGAISKTIN
                     LPHEATVEDIEECYMLSWQMGLKANALYRDGSKLSQPLSTKSDSKEGSDEKVKHEWTS
                     EEVLEAARSIIMQSTDTIFKRQLSRVVERKKLPAKRDGFTQKSKIGGQTIFVRTGQYE
                     DGTLGEIFIDMHKEGASYRSLLNCFAIAVSIGLQYGVPLEEFTDKFTFTRFEPSGPVD
                     HANIKFSTSIVDYVFRLLAFEYLDRTDLVHVAPEEMSQFEKNPQDISTETTGNYPVTE
                     PVAEKQPKSDSANRKASAAKYQMANAQEAANQSLMGDAPPCPKCGHTTIRNGTCYKCL
                     NCGESLGCS"
     gene            6006..7013
                     /locus_tag="PLL28_01570"
     CDS             6006..7013
                     /locus_tag="PLL28_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68029.1"
                     /translation="MNRKNFLRGLSLAGVGLTLPSSSVFAKPQKNEITGGCILIPTET
                     AGPYPLDLSENAFYFRNDVREIQTGTQLNLKIKVIGNSDCMPMQNVRVNIWHCNKDGL
                     YSGYDNASNPGQADFTYLRGYQIADANGEVNFITILPGWYNGRVCHIHFRVYVSTAYA
                     AISQLTFDHNTVNAIYAESPDLYTKGADPLTPETDGIFADGYAYQLATLTVNPDTGGY
                     DSYLEVTVEGQGTTGIGWQENVNAQHFSLQQNFPNPATEQTTIPFVLKTASSVQIELY
                     DINAQKIKTIDLGDLPPGNHESQINFEALSLPKANYIYQLVVQNNAGRFTDHKLMTVA
                     K"
     gene            complement(7037..7828)
                     /locus_tag="PLL28_01575"
     CDS             complement(7037..7828)
                     /locus_tag="PLL28_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNF68030.1"
                     /translation="MNIQLYPYTAMVCGSTQGIGKACAFELASMGARIVLVARDADKL
                     NQVKQLLPNPHGVEHESIIADFSNPEQVRAAMHAYFSSGEKTVNILVNNTGGPASGKA
                     SDAETEQFLAAFKQHLICNHILATACIPGMKNSGYGRIINIISTSVKAPLPNLGVSNT
                     IRAAVANWSKTLANELGAFGITVNNVLPGATKTERLTGIISANAARTGNAVDAVEHKM
                     LEEIPMKRFGAPEEIANAVAFLSSPAAAYINGINLPVDGGRTASL"
     gene            7884..8402
                     /locus_tag="PLL28_01580"
     CDS             7884..8402
                     /locus_tag="PLL28_01580"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF009763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HNF68031.1"
                     /translation="MAIAGPLNLKQWIDDNRHLLKPPVGNQQIYKMNEDFIVMVVGGP
                     NSRKDFHFEDGEELFYQLEGDITVAIQEDGKRRDIVIKEGEMFLLPPNVPHSPMRPAN
                     TVGLVIERYRTEKEFDSCMWFCEKCNHKLHEETFPLQDIVNQLPQIMGKFYADLDLCT
                     CKSCGHVMEPPK"
     gene            8491..8850
                     /locus_tag="PLL28_01585"
     CDS             8491..8850
                     /locus_tag="PLL28_01585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNF68032.1"
                     /translation="MTKQILKAFYTVYNALGYGFLEKVYENALKIELEQSGLQVLQQE
                     SISVFYDNAKVGYYVADMIVGKYDIVENKACSALNSDHEAQLLNYLKATKIEVGLLLN
                     FGLKPEFKIKIFENKYK"
     gene            8902..9915
                     /locus_tag="PLL28_01590"
     CDS             8902..9915
                     /locus_tag="PLL28_01590"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNF68033.1"
                     /translation="MLKIDIHTHIIPEHLPKWSEKFLDNRYIHLDHYCPGCAKMMQGD
                     KFFREVKSNCWDGNVRIEECDATNVNVQVLSAIPVMFHYWEKNAAYALETSRFQNDFI
                     AEVVSRHPTRFIGLGTVPLQDPQLSIEEMERCVKELGLAGIEIGSHVNDWNLNAEELF
                     PFFEAAEKLGAAIFVHPWDMMGKDKMNKYWLPWLVGMPAETSLAICSMIFGGVFEKLP
                     NLKVAFAHGGGSFPSTIGRIEHGFNVRPDLVAVDNKVNPRTYLNKFYLDTLVHDDQML
                     NYLLDLFGPAQLALGSDYPFPLGEHHPGKLIESMPYDDSIKARLLHGTALEWLGLEKG
                     QFE"
     gene            9969..11240
                     /gene="kynU"
                     /locus_tag="PLL28_01595"
     CDS             9969..11240
                     /gene="kynU"
                     /locus_tag="PLL28_01595"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HNF68034.1"
                     /translation="MSVTFTADKQLAAQLDAADPLKDFRQRFYIPSQDGKEQIYFCGN
                     SLGLQPKSTRSFIEQELIDWQHLGVEGHLHGKNPWFYYHHFLAESTARIVGANPLEVV
                     VMNSLTVNLNLLMISFYRPTKQRYKIIMEYMAFPSDQYAVENQVKFHGFDPFDAIIEL
                     MPREGEAALRTEDILATIEQHKDSIALIMLGGVNYYTGQLFDLSTITAFTKKCSSEIV
                     VGYDLAHAAGNVMLSLHDWGVDFATWCSYKYLNSGPGGTSGVFIHEKHAQDNSLPRLS
                     GWWGNEENTRFKMQKGFFPQPGAAGWQMSNAQILPMAAHRASLEIFDAAGMDNLVQKS
                     KLLTGYLEYLLLSGARKDFKIITPADHAQRGCQLSIVMEHNGKAVFDKLTENGIIADW
                     REPDVIRVAPVPLYNTFEDVWRFADIFHSAL"
     gene            complement(11309..11875)
                     /locus_tag="PLL28_01600"
     CDS             complement(11309..11875)
                     /locus_tag="PLL28_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diheme cytochrome c-553"
                     /protein_id="HNF68035.1"
                     /translation="MHTRITLTIVCAGLFALSACTKSADCPEPEVKAEDPVQRGQFMV
                     TVGGCNDCHTPKNMTPQGPVPDMSRMLSGHPSGEPVAPYDKNTVGPWVLLSPGLTCAV
                     GPWGTSFAANLTPSESGIGSWSEEQFVKALQEGKHMGMDNTRPIMPPMPWQEIGKFDV
                     NDLKAIYAYLKSIPPVDNVVPAYMPPVQ"
     gene            11992..13332
                     /locus_tag="PLL28_01605"
     CDS             11992..13332
                     /locus_tag="PLL28_01605"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNF68036.1"
                     /translation="MQVKNIAIMGAGLVGSLLSMYLKKHGYQVTVYERRPDYRKAENY
                     GGRSINLAISERGWKGLAGVGLEEMVREIAIPMPGRMIHDVQGNLNFQPYGKPGEAIN
                     SVSRGGLNVMMIDAAEKAGVTYLFEQRIMEIDPSGKKAMMEDMSTGRHYTLEPDLIIG
                     ADGAYSLVRYAMLKTDRFDYSQAYEAHGYKELTIPAGPNGSFLIDRDALHIWPRKQFM
                     LIALPNIDGSFTCTLFFSLEGKESFEAIKTLADLDAFFDTYFPDVKSLIPDLHHDFFA
                     NPTGLLMIVKCFPWSHGNTMLIGDAAHAIIPFYGQGMNCGFEDCTVLDTLIEEFNHDW
                     SKIMPAYERLRKPNSDAIADLAKLNFIEMRDLVADADFQFKKRIAAKVSALHPDRFIP
                     VYSMVSFSHLPYKDALSEYHRQDAVLTEILGWPEIENRWETDYLPQIAERLFNK"
     gene            complement(13520..13882)
                     /locus_tag="PLL28_01610"
     CDS             complement(13520..13882)
                     /locus_tag="PLL28_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68037.1"
                     /translation="MKIKFLLFVLAGGFVLASCSDKEAEQKIADLEKQMHVGDSACAA
                     EKTALMDSIAALHMEMEEWMKMDAMKSSTSSKSSTSTKSTTTVTPAPDKKVDVNVKGG
                     DTKKTIDIKKKGGATGGN"
     gene            13985..14686
                     /locus_tag="PLL28_01615"
     CDS             13985..14686
                     /locus_tag="PLL28_01615"
                     /inference="COORDINATES: protein motif:HMM:NF027718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HNF68038.1"
                     /translation="MKTKISVLSLAVIAAGGMFITSCGTEDLSVPVIVLEGDSPFIVE
                     LGDTYTDPGFTATDDEDGDITASVTVDDSDVDTEEIGEYEVTYTVTDGAGNVGTETRI
                     VRVVMGKADYMGTFQVHEICDMDGDGVYGEPGVDYEINDYTVTVTSGGDDNEVLFQNF
                     GAYGATVIVPVYFSGDLNEILTVDDYNLPGTTIYFNADGEITTGTTSDIEFDLDYNAQ
                     DGATIIPCQATFVKL"
     gene            14787..15416
                     /locus_tag="PLL28_01620"
     CDS             14787..15416
                     /locus_tag="PLL28_01620"
                     /inference="COORDINATES: protein motif:HMM:NF027718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HNF68039.1"
                     /translation="MRKLLPISIVIIFFTSACTRDDSTAPLILIQPDAHAPHYRTIPF
                     VDPGCEVADDYSCADAIDLQISNNVDVNKYGTYNIMYTATDEAGNINEYNRPVDIILP
                     ISDYYDFAYSAYDTCTSGNYFYTGLAQDCDCPDDKVIFGNISNFGPNATFPLPINGQY
                     NHMITLDTTKLAVTFFGTGVMSPAADTIFWEYVIMDSISTDACRSVWIK"
     gene            15448..17106
                     /locus_tag="PLL28_01625"
     CDS             15448..17106
                     /locus_tag="PLL28_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNF68040.1"
                     /translation="MTTLNTIFSRIFLGILITVSHSVLAADPGRINGQIIVQFYSNVE
                     PERVIIDVELASKTDVALLKPLSASLNIWLIAFDENVYNAEFVKDAFFKNPDVALVQF
                     NHTFSERSIPDDPFFYQQWGLLNDGAGGIADADIDADLAWDITTGGVTAKGDTIVVAL
                     ISEGQFYAHEDLDYFINRNEIPDNDIDDDANGYIDDYHGWNGTTQTDSVPERLHGTHV
                     AGIAGAIGNNGLGISGVSQKLKILPVYNLTVEADAVASYAYARDMRKIYNETNGEKGA
                     YIVATNSSFGVDFGNPDDYPIWCAMFDSLGVVGVLSVASTANTSVNVDNVYDIPTACP
                     SEYLITVTMTDKADNLFSAAYGAKSIDLGAPGNLIYSTIIDNSYGILSGTSMSAPHVT
                     GAIAMMYAAACPEFFDALEDNNELMLLKLKQYLLEGVDVVPDLEGVTVSNGRLNVFRA
                     IENLTVSGFCATGFAENALGVAYPNPAQELLFINGATIIDQEVQVQVVNTLGQVVYQR
                     TGDRSPFIFSGIPVGDLPSGYYAVSVYNPDSDLLFGSGVIIQHQ"
     gene            17123..18823
                     /gene="recJ"
                     /locus_tag="PLL28_01630"
     CDS             17123..18823
                     /gene="recJ"
                     /locus_tag="PLL28_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNF68041.1"
                     /translation="MDKRWVIKVTEEEKVQSLQQELKIHPVICNLLVQRGITTYQEAK
                     QFFRPSLDDLHDPFLMRDMEKAVKRITEAINNQERILIFGDYDVDGTTAVALMYAFFY
                     SRYRNIDFYIPDRYKEGYGISFQGIDYAKEHQCTLMIALDCGIKANDKAAYAKELGID
                     LIICDHHLPGDILPDAYALLDPHRPDCSYPYKDLSGCGIGFKVVHAFAKYHDIPVEQV
                     ISYLDLVVVSIASDIVPIMGENRILAYYGLEQLNKNPRPGLKALIDITGYTPPLNISN
                     IVFGLGPRINAAGRMDDANQAVKMLISENVDAAKNGADVLQEKNVDRKEVDKMITADA
                     LDILATDPLTPGRVSTVLRSSNWHKGVIGIVASRLLDHYYRPTIMLAEKDGMLFGSAR
                     SVKNFDIYEAIKACSDLLEQFGGHMYAAGLSMRQENFEAFRDKFDHVVRERIRPEQLI
                     PEIEIDSTLELKDITPSFFNIVRQFAPFGPGNMEPVFISEQLMNTGRSKIVGDGHLKL
                     AVRDSRRFTADGIAFQKGHHFPLIADGKRFDICYTIEENTFRDMTNIQLNVRDIKVNV
                     "
     gene            18846..19619
                     /gene="lptB"
                     /locus_tag="PLL28_01635"
     CDS             18846..19619
                     /gene="lptB"
                     /locus_tag="PLL28_01635"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNF68042.1"
                     /translation="MQSMIVRAEKLIKKYKQRTVVNEVSVEVNQGEIVGLLGPNGAGK
                     TTSFYMIVGLIRPLSGKVFLDNMDITSFPMYKRAQLGLGYLPQEASVFRKMSVENNIA
                     SVLQMTNLSHKEQKAKLESLLDEFGLKHVRKSNGDVLSGGERRRTEIARALATDPKFI
                     LLDEPFAGIDPIAVEDIQLIVEKLKFKNIGVLITDHNVQETLSITDRAYLLFEGKILK
                     QGTAEDLAGDPQVRKVYLGQNFVLRKSRATTNTTQPPVE"
     gene            19729..21171
                     /locus_tag="PLL28_01640"
     CDS             19729..21171
                     /locus_tag="PLL28_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNF68043.1"
                     /translation="MSDPIRAQDQVFQTLIDGGRDTVWGKQYDYASLRSLNDLKNRFP
                     VQDYDTLKPYIDRIMQGEQQVLWHSHIAWFAKSSGTTSDKSKFIPVSSMALEECHFRG
                     GRDVIAMYLHNNPESKLFDGKSLLMGGSHQINKLNQHSKYGDVSAVMMQNLPWAAKYA
                     MTPSLEIALMDEWESKIEAMAHYTKNDNVTSIAGVPTWTIVLIRRLFELCGTDNVKDI
                     WPNLELYIHGGVSFKPYRSQFNTLIRKDNMHYVETYNASEGFFAFQDRLGADDMLLMP
                     DYGLFYEFIPMEEFDKEDPKTLSWGEVELGKNYAVVVSSNAGLWRYKIGDTIRFTSLY
                     PHRVQVSGRVKHFINAFGEEVIVDNSDKAIAEASAQTGAKVVEYTAAPIYISGNEKGG
                     HEWIIEFSTPPENIEQFTAILDATLKSINSDYEAKRHKDLALKMPVVHIARKGAFYDW
                     LKSKGKLGGQHKIPRLSNERTYVDEILKFL"
     gene            21251..21994
                     /locus_tag="PLL28_01645"
     CDS             21251..21994
                     /locus_tag="PLL28_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNF68044.1"
                     /translation="MQRYTAILVDDEEHGRRNLQTLLEKYCPEIEVIAEAAGAAEAKE
                     KILSLTPQVLFLDINMPELNGFDLLESIAKKDFAVVFVTAHRDFGIEAVKAGATDYLL
                     KPIVIKELQQAVKKVVMYYDERSAEANNADVPKPERITLGHTGGFSVVEMKDIVRLEA
                     ESNYTRVHVTGKKSYFVSKPLKVFEDSLKDNIFFRVHRSYIINLNHVKEFLREDGGVI
                     VMLDDARIQLPKARYNDFLEAMRKLSIAI"
     gene            22050..24890
                     /locus_tag="PLL28_01650"
     CDS             22050..24890
                     /locus_tag="PLL28_01650"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNF68045.1"
                     /translation="MHVKAQIFPARQYTTRDELANSNVYDICHDDRGYLWFATERGVS
                     RFDGYKFTNYLFKEGLDGAIVFSLSKDARGNILAATKFDGAFKMSGNFNKIIEEDLLL
                     ANEQTVLAGKYFYSLKDGVRVAVYQMGNNKTSVLSFPGGAKPYFILQKDSSAVYIGTS
                     SGVFISKNGKTPERVFPEINEPVHSLAIRGNTVFVGLQGAVYAIADNAVSVAALVPDG
                     GKVKRLMVDDNNRIWCATFPENRLYMFDKNTAIDISQKLNIAGISVNKILEDNEGNVW
                     IATYGKGVFCLHHMYCTNYTSYDGLKNEYITSLLPIEHELLIGTYNGLFGNTNNQITP
                     LKSFNNELEFIRKLDIHDDLIIATVSGISADKVLIKRSTIGNHPLQYVHATTTFIDGN
                     YIYYSRWDKKVWRAQINGTEIGDAEVVFEDKEGEWTRVNAIFRDPQNKLWVGTTRGVY
                     IISANNSWVRNDTGFLRTNITAFALDHDGSVLLGTDKGLVRYNDGKWIASHEINGKNL
                     ENITGLAHDNNDRIWISTLNGLFLMAENELIQFDARNTLLSDEINALAYDASENAIWV
                     GTSFGLSRIDVGLFDKAVVIAPAAIFKTLRAADSIYRDLGINGRLELPYTSKNLSVRF
                     SAIQFAAPEGIKFFYKFDDGPWEPTTGRQIEFASIPYGEHTIQLKSVGERGVEGPVAN
                     LTILVTTPIWATAWFKLLIGLTIAGIAYFLLRKRFEIQRKKQQERLELQSKVAELRHQ
                     ALAASMNPHFIFNALNSIQHFINSHNTEEATDYLGKFARLIRMMLDYGGRTFIPLKDE
                     LERLEYYLELEKVRFGEKLNYSIEVDDSLRNNPLEIPNMVIQPVVENALWHGLLPANR
                     NGHLLVKFAQRDNVIFVTVDDDGIGIIESKRRKKSSHNSLGIQMIRERLELLNRLSGY
                     IAEIEIKDKSEINADKEGTIAQIVMGGKKEQQ"
     gene            25151..27415
                     /locus_tag="PLL28_01655"
     CDS             25151..27415
                     /locus_tag="PLL28_01655"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNF68046.1"
                     /translation="MQIMEANRTIALQNGVLLSGDNDWVGAILHTSDTFYNCNVRLKG
                     DWVDHLQGDKWSFRIKLGSDDAINGMRVFSVQDPITRDHLSEYVYHQWLAAEDILTTR
                     YDFMPFTLNKGKTYTYAVEEHFDINLIESQHRRAGPILRFSEEFLWDLRVQDSAAFKS
                     GGMYENNIDVFAITDILSFSDKGLGKDVELTKQYEVARTLMQQYRAGKVALADIFDMD
                     KMAKYFAIVDINQAYHSLIWHNMRFYFNPITQKLEPIGFDGFTEYGVYDNIKRPFIGY
                     SASYQYRQKIEDDALTDRFFNDFEFSNTYAKYLHKFADTNYINAFFDSIGESLTEKGN
                     LIRKDYPEYQYNKKHIYDRTLAIEINLKPQGDYTLRAFADMAAPAKIGIINHVAVPVS
                     VLGMSIRPDTIMYYLDKPINLPAWNEYKAPPFAAVKKIGESQYLFYKVAGTDSLVSVK
                     ISPWEDDLVQTPEQRLFNTTISSNELYVIKNNEIFFKPGLHVVTKPIIFPAGYNIHIS
                     PGTQLDLRNGAMFVSRSPLSVIGTESSPIKIFSSDSTSMGFTVLQAASASAFQYVIFD
                     HLSALDHEGWKLMGAVNFYESTVSLEHCRFTHSQCEDALNIMRSNFSIDYSYFANTYG
                     DAFDSDFSTGTVKNTYLTHTGNDALDFSGSFIEVKRCELVHLGDKGMSCGEDSKITIS
                     DCSIKDARIGVAAKDLSRVEVQNLQISDSGIGYLAFVKKQAFGKSSIQVTGGTLTNVK
                     QTTSVLDGCEIIMQ"
     gene            27514..28047
                     /locus_tag="PLL28_01660"
     CDS             27514..28047
                     /locus_tag="PLL28_01660"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor"
                     /protein_id="HNF68047.1"
                     /translation="MPYLCTTQKRGHQKVPSFVSVTMGNREQIEEWLGPKLEELGCFL
                     VDIRINPSSSKYEVYIDTPTGVTIEQCEKVSRHLSFMIENQGGFPEKYTLDVSSPGMD
                     NPFKVQQQYDKTIGKTVEVLLLSGVKMEGMLKKADGKVLHLEVHHPPKKKGMKPEITL
                     ETYDFSEIKHVKKKINF"
     gene            28113..29354
                     /gene="nusA"
                     /locus_tag="PLL28_01665"
     CDS             28113..29354
                     /gene="nusA"
                     /locus_tag="PLL28_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNF68048.1"
                     /translation="MNSIELVESFSEFKDVKNIDRPTLMKVLEDIFRTLLRKKYESDE
                     NFDIIVNTEKGDLEIWHRREIVEDGMVENPVTQISLADALKIEPDFSVGEETYEEVSI
                     ESFGRRAILAARQALVQRIMELEKDEVYKKYKDREGEIITGEVYQVWKKEILLLDDDG
                     NELILPKGELIHSDNYKKGDAIRGVVKKVDFRNNNPVVIVSRTDPKFLEKLLEQEVPE
                     VFDGLIVIRRIVRAPGERAKVAVESYDDRIDPVGACVGMKGSRIHGIVRELRNENIDI
                     INWTNNNQLLIQRALSPAKITSIELDNDRKKAAVYLAQDQVSLAIGKGGLNIKLACSL
                     TGYDIDVFRDNEEADVDIDLDEFADEIDEWIIDELKAIGCDTAKSVLELSVDELVRRT
                     DLEEETIKEIVRILKAEFDED"
     gene            29417..32221
                     /gene="infB"
                     /locus_tag="PLL28_01670"
     CDS             29417..32221
                     /gene="infB"
                     /locus_tag="PLL28_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNF68049.1"
                     /translation="MSEEKSIRLAKAASEFNVSLAHIVDLLKTKGFSVEPKPTSKISE
                     EMHNILLKEFGKDKDLKGKADQLNIGIGRKLEKEKQEKEEAEALQEKPVQEVVVEEEK
                     VVAPKVTKEKKVEEEVKAPEPEPEPEPEPEAPAKEEKDELIKMERGKVGLTVVDKIDL
                     NKKKAPKKTVAPEEKPVVEKAKSDKKEKVEEVKPVVEKQEIPVAKQVTEVPEKKPEAE
                     MIETKRVTLEGPKIIGRIEIKEEKKPEKRGPDNNAEKKKRKRIIKHERINVDKVKEEE
                     RQRENRNRHQGGQGTRKPEVDSEEAQRQIQEQIRQTLAKLTGGGQQKGRGSRMAKQMK
                     RERREAAEIEAEAASHSNQLMVAEFTSLSELASLMNVSPTSLVQSCFNLGMMVSINQR
                     LDAEIIELLAEENGFKVQFINATEEETEEDDIPDDPADLETRPPIVTIMGHVDHGKTS
                     LLDYIRETNVVAGEKGGITQHIGAYEVDLPNGKRIAFLDTPGHEAFTAMRARGAKLTD
                     IAVIVIAADDQVMPQTKEAISHSQAANVPMIFAINKIDKPGANSDKIKEQLSQMNILV
                     EDWGGKFQSQEISAKSGLNVETLLEKILLEAEILDLKANPDKEAVGTVIEASLDKGRG
                     YVSNIMVQEGTLKVGDMMVAGPFFGKVKAMFNERNQRITEAGPSTPLLVLGLNGAPQA
                     GEKFKIFEDETQAKSVANKRSQILREQGIRTKKHITLEEIGRRRALGTFRELNIIIKA
                     DVDGSTEALTDSLQKLSTKEVQVNVIFKGVGQITESDIMLASASDALIVGFQVRPSSS
                     VRKLAEKEGVEIRLYSIIYNAIEEVKAAMEGMLAPKMEEKVICMVEVRDTFKISKVGT
                     IAGCYVTEGKITRNTKIRLVRDGIVIHTGSLSSLKRFKDDAKEVTTSMECGLTINNYN
                     DIQPGDMIEGYEEVEVKRSL"
     gene            32710..32922
                     /locus_tag="PLL28_01675"
     CDS             32710..32922
                     /locus_tag="PLL28_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNF68050.1"
                     /translation="MSNVLLLSMPGGMEWVFIILAVLLLFGGKKIPELMRGLGKGIRE
                     FNDAKNNVSREIREGMNEEPKKSEEK"
     gene            32938..34383
                     /gene="gatA"
                     /locus_tag="PLL28_01680"
     CDS             32938..34383
                     /gene="gatA"
                     /locus_tag="PLL28_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNF68051.1"
                     /translation="MSAVSDSLLRSGLHAVQADLRAGTISVRQIVEHYLRVNRDKQHL
                     NAFLEIFEHDALHQADEIDARVRSGKPMGKLFGMVIGVKDVISIKGKRLTASSKVLDD
                     FESLYNATVIDRLRHADAIILGRCNCDEFAMGSSNENSAFGPVLNDLDNTRVSGGSSG
                     GSAVAVQAGLCMATLGSETGGSIRQPASFTGTIGFKPTYGRLSRYGLIAFASSFDQIG
                     PITNTVEDMALMMEAMAGQDPHDATSSPEPVSHYSEHLDAPKKLRIAYYPQTLDNPKI
                     DPEISAAMYGLIDKLRADGHVVESCDLKYLDYLVPTYYVLTTAEASSNLSRFSGIHYG
                     FRDMEAEDMESVIRRSRSKGFGKEVQRRIMLGTFVLSSGYYDAYYTRAQKVRRIIRDD
                     TAETLSSYDLILLPTTPTTAFKLGEKTTDPIEMYLADIFTVQANITGLPAISVPLFRH
                     SNGMPFGAQLMGNYNSEELLFSVTSYLLKNY"
     gene            34595..>36050
                     /locus_tag="PLL28_01685"
     CDS             34595..>36050
                     /locus_tag="PLL28_01685"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNF68052.1"
                     /translation="MDHLDSLLEAFYDSNPAWRFDEQDYALNGLAVTDTPRFTPEVIA
                     DRISKINSPIPYTYNKSVQTFIDLYTIKRRTQVSNMLALSQVYFPMFEEELDRRGMPL
                     ELKYLPVIESALNTHAVSKAGATGLWQFMLGTAKLKGLRIDTYVDERRDPHMATIAGC
                     QYLQDLYNIYGDWLLAIAAYNCGPGNVNKALRKAGGGDKTFWDIEQYLPKETKAYVPI
                     FIAATYTFEYHKEHNIRPATFGYDFSMSDTLMITHKMTIDQLAPYVGISADEIALNNP
                     ALKTKTIPGSPVPYPLILPQKAVAAYYANKDSLYASLERKEQIASQQLAKNVAAVNAA
                     NDKLKPKPATVAGTGDSSVTTATATPINTTTPDLTLADPDAPVTVNYTVKKGDNLGYI
                     SDWFDCTVADLKKWNKLSSTQIVPGQKLKITVPAKYEQQYAQINNMTLSQKQKLTDIA
                     LISPASTTDKGPVVTNDIIFYTVKPGDTLWSISQA"
BASE COUNT        10800 a         7156 c         7962 g        10032 t
ORIGIN      
        1 tctcctctct cctctcatat catttccact tccatgatca atattctgct tccctcactc
       61 aatgctttga tttgatgtgc gcttaattca gttaccccca ttccatcgcg acgatcaagt
      121 ttttctccat ttatttcgaa gctgccttcc aacacgaaaa agtatgttcc gtttgtggcg
      181 tcatgttgcg tatagtttgt tgtagtccct ttatcgaatg tgccaatatg aaaccacgca
      241 ttttgatgaa tccacacgcc ttcatcgtcg gcatttggac ttacaatttg cgtcaacgtg
      301 ttgatgccgg ctgccggatc caatgtgatc tgatcatatc ttggtgctac ttgctgctta
      361 ttgggtatta cccagatttg caggaacttg acctgtttat cggcatgcgc attttgctca
      421 ctatgaaata tgcctgttcc ggcactcatc acctggatat cgccctgttt tatcacagca
      481 acgttgccca tgctgtcctg atgacgcaaa tcgccttcca aaggaataga gatgatttcc
      541 atattgtcgt gcggatgctt cccaaatccc attccgccgg aaaccgtgtc gtcattcaat
      601 acgcgcaaag ctccgaaatg caccctgtcc ggatcataat aatgtccaaa gctgaatgta
      661 tggtaggtat ccagccaacc gtgattagca tgtccccttg aaattgctgt gtgaatgatc
      721 tttttcataa aatatatgta tatacatata tgttgataca aatatagcaa acagaggtaa
      781 aatgcaaata gttcattcag ggtaaaaaaa catcagaaat gcttgtcagg agccgtgttt
      841 ttagctgatt acctgaataa aattctatct tcgcactcag gcacacaaca cacctcatga
      901 acatgaaagg cttaattccg gcattgttgg tcggtttatt ttccacagca ttacaaggac
      961 agattcttac aggggttacg ctcgaaggcg agatttatcc ggcacacgaa gatctgatta
     1021 cggatccggg tttttttatt ctcgattgtg ataatgattc acttggcgca ctgcctttgg
     1081 tggatttggg aaccggatat tatatgggtt accagggtgg cttatttccg ggaggttcaa
     1141 ataacgatcc gaatccgcat ttcaacaatg gcaaaaacat ttcgaaagca atcaagccac
     1201 ttaatgattt aggtctggta gattatctca ccggcgaagt gtctttaatt ggaatcggcc
     1261 cctcggttgc gagtgatcca tataatgaat ggaaagaaac gcagaatgat atcgactggc
     1321 ccggtgttaa tccttgttta aatgtgaagg gtaattttat cggcggcaaa agtgttgccg
     1381 atatgttgga tattgacggc acctattggg caagttttct aacggggctc gcaagtaaaa
     1441 acattgaccc acttcaagtt caaattgcat ggatgttgct ggtgtcgtcg acagattcga
     1501 atgatgtcaa ttattatgtc gacacagtaa ccaatcaata cattcaggta ttacataatt
     1561 tgcaaaccct ctgcccgaat ctgcagcagg tttttatttc aggaatacat tataccggat
     1621 atacatctcc cgaacacaag cgctatgatt atctgataga accttatggt tactggagca
     1681 atcttgtagt aaaaaatgta atcacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnngcgca tgatgtaatt caaattccgg tagatgaaaa tttgcaaggt gaatcaaccc
     1861 tgatgatgta cgatcaatat ggaagtcttt tatgtgtaca acattttgat gcacgcacta
     1921 ccttaacttg cgaagttcaa attaataatc tgccttccgg aatttattat gtgttgatgc
     1981 gagatcacaa acaaacaatt gcaggaagat tcttgaaaac ataaacgaaa caatttcttt
     2041 tcaatcgtta tacatttcct tcaaatttct ttaatgccaa aaatttaatt tgggtgcaat
     2101 tttcgtgcag attcatcgtt agtttttaac gaatgcacat caatccacag cattaaaaca
     2161 catcaaaaat tttaatctgt ttgtgttttc tatttgtggt ggtaaacgag agtaaaaagg
     2221 ctgttttttt caattttccc cctcgatgcc gctctgccac tgatttgagc cattccttct
     2281 tattcatatg caaagaaaat ttcaaatttt tcgaccgaat tttttcccca aaatcgatgt
     2341 ttatttcaac atcaatgtgg acagacggtg taaaaattac tttaaaaaaa aatatatgtg
     2401 aacaacgcga cgcccacggt acaagactaa tttcatctta cccacaaggg ggtggaaaag
     2461 tgttacttgt ttgtaaacag tttcatgtca tactttcgtg aaacctctcg agagaaccgg
     2521 gaggtttttt cgtcagtacc tgaaccaaac tgtcgaaacc ccttttacca acaaaccgaa
     2581 acgcattaat cagcatggcc agcaaaaaaa aacctacaac gggcggatta tcatttgagc
     2641 gctactttac tcaggatggt gtaagtcctt acgaccagtt cacctatgaa ctgcgatcat
     2701 cagttatcag aaataccacc ggcgacgcgg tatttgaaat gcataatgtg gaagtgccac
     2761 aagactggag ccaggtggct accgatatat tggcacagaa gtatttccgc aaagcaggag
     2821 tgccgcaagc tgacggcagc acgggaagtg agacttctat taaacaagta gcccatcgcc
     2881 tggccgactg ttggcgccaa tggggtgagc aatatggtta tttcgcgtcg tctaaagacg
     2941 gacagatatt ttatgaggag ttggcataca tgatcctggc gcagatgggt gctcctaact
     3001 ctccacaatg gtttaatacc ggtttgtttt ccgcttatgg tatcaaagga aaacctcaag
     3061 ggcactatta tgtagatccg gattcaggtg aattgaaaaa atctacttcg gcctacgaac
     3121 gtccacagcc tcatgcatgc tttatcctta gcgttgatga tgatctggtt aaccagggtg
     3181 gtatcatgga cctctggaca cgtgaagcac gtatattcaa atatggctca ggcgttggta
     3241 ccaactattc aaaaattcgt ggcgaaggag aaaaactttc aggtggcggt acttcaagtg
     3301 gtctgctcag cttcctgaaa attggcgacg ctgctgcagg tgccattaaa tcaggcggaa
     3361 ctacacgtcg cgctgcgaaa atggtgtgtc tcgacctcga tcaccctgaa attgaatctt
     3421 ttgttgagtg gaaagttaaa gaagaagata aagttgctgc tttgatagct gcaggttatt
     3481 catctgatta tgaaggtgaa gcataccgca cagtagcagg tcaaaactca aataactctg
     3541 tgcgcattcc taacagcttc tttaaagtat tggaagaaga aggtcaatgg gaattaatcg
     3601 gtcgcacaaa tggaaaagta tttaaaacag tatctgcacg taaactttgg gataaaattg
     3661 catacgctgc atggcgttgt gccgatccgg gtgtgcaata cgatactacc atcaacgaat
     3721 ggcatacttg tccgaaatca ggacgtatca acgcatctaa cccatgcagc gaatacatgt
     3781 tcctcgataa tacagcatgt aacctcgcat cactcaacct ccgcaaattc tacgacgacg
     3841 ataaactcgt tttcaatgta gatgcatttg aacacgcttg tcgtgtgtgg acagtagtac
     3901 tcgaaatttc agtattgatg gctcagttcc ctagccctga agttgcacaa ctctcttatg
     3961 agttccgcac attgggtctc ggatttgcca acttgggttc actgttgatg gtttccggta
     4021 ttccttacga tagcgataaa gcccgctcaa tcgcaggtgc tatttctgct atcatgaccg
     4081 gtatctcata caagacttca gccgaaatgg ccggagcact tggtgccttc aaaggttatg
     4141 agttgaataa agaagacatg atgcgtgtaa tgcgcaatca ccgttatgct gcttataatg
     4201 ctgctgataa ttatgaagga ctggaaatta aaccaatggg tatcgaccag gcactttgtc
     4261 ctgaatattt gttgaaggcg gcatgcgatg catgggatga agcattgcaa ctcggtgata
     4321 aattcggata tcgcaaagct caggcaaccg ttattgctcc aaccggaact atcggcttgt
     4381 taatggattg cgatacaaca ggagttgaac cggattttgc attggtgaaa ttcaaaaaat
     4441 tatcaggtgg cggatatttc aaaattatca atcagtcgat tccgctcgca ttgaaaaatc
     4501 ttggttataa agatgaaagc atagagtcta tcgtgaatta tgccaaaggt accggaacat
     4561 tgaaaggtgc gccaagcatt aattacgata cattaaaaca aaaaggattt accgaaactg
     4621 aaatcgacca tgtagataaa gtgatggctt cagctttcga aatcagcttt gctttcaacg
     4681 tattcacact tggtgccgaa acactcgaac gccttggatt cagtgctgaa cattatagtg
     4741 agccgggatt taatctgctt cgcgcattag gatttactcg cgaagaaatt gaagctgcaa
     4801 acgatcacat ctgtggaact atgaccatag aaggtgcacc ttacttgcag gaaaaacatt
     4861 attcagtatt cgattgcgca aataaatgcg gaaaaatagg cgagcgttat atccatcaat
     4921 taggtcacgt tcgtatgatg ggtgccgtgc aaccatttat ttcaggtgca atttctaaaa
     4981 ccattaacct tccgcatgaa gctacagtgg aagatatcga agaatgctat atgctgagct
     5041 ggcaaatggg cctcaaggca aatgcattgt atcgcgatgg ttctaaattg tcgcaaccgc
     5101 tttcaaccaa aagcgatagc aaagaaggca gtgatgaaaa agtaaaacac gagtggactt
     5161 ccgaagaagt attggaagct gcacgcagta tcatcatgca atctaccgac accattttca
     5221 aacgtcagtt gagccgtgtg gtagaacgta aaaaattacc ggcaaaacgc gacggcttta
     5281 cacaaaaatc caagatcgga gggcaaacta ttttcgtgcg cacaggccaa tacgaagatg
     5341 gtaccctggg agagatcttc atcgatatgc ataaggaagg ggcatcgtac cgttcactgc
     5401 tgaactgctt tgccattgct gtttcgatag gcctgcaata cggagttccg ctggaagaat
     5461 ttaccgacaa attcacgttc actcgttttg aaccaagcgg cccggttgat catgctaata
     5521 tcaagttcag cacgtccata gtggactatg tgttccgcct gctcgcattc gaatatctcg
     5581 atcgcacaga cctggtacac gtggcgccgg aagaaatgag ccagtttgag aaaaatcctc
     5641 aagacatttc aaccgaaacc accggcaatt atccggttac ggagccggta gctgaaaagc
     5701 aaccaaaatc tgattctgca aaccgcaagg cctcagcagc aaaatatcag atggcgaatg
     5761 cacaagaagc tgccaaccaa tcgcttatgg gcgatgcacc tccgtgtccg aaatgtggcc
     5821 ataccacgat ccgcaacggc acttgttata agtgtttgaa ctgtggcgaa agcttgggtt
     5881 gcagttgatc ggacaaacaa atattaaaaa ccgccttcct gtgatggcgg tttttttatg
     5941 taccttcatg atggtattcg ccggtgacag tggcaaatac atattcaggc aacttaattg
     6001 acatcatgaa cagaaaaaac ttcctcaggg gcctcagcct cgccggtgtg gggcttactc
     6061 taccgtcatc ttcagtattc gcaaaaccgc aaaaaaatga aataacaggg ggctgtattt
     6121 taatcccaac agaaacagcc ggtccatatc cactcgatct atctgaaaat gcattttatt
     6181 tcagaaacga tgtgcgtgaa atacaaacag gaacacaact gaatttgaaa attaaagtaa
     6241 ttggaaattc cgattgcatg ccgatgcaaa atgtgcgcgt aaatatttgg cattgcaata
     6301 aagacggtct ttattcagga tatgataacg catcaaatcc cggacaggca gattttactt
     6361 atttgcgcgg atatcaaatt gccgatgcca atggtgaagt aaattttatt accattttac
     6421 ccggttggta taatggtcgc gtttgccata ttcattttcg ggtatatgta agcacagcat
     6481 atgcggcaat ttcacaactt acatttgatc acaacacagt aaatgcaatt tacgccgaaa
     6541 gccctgatct gtataccaaa ggggccgatc ctttaactcc ggaaactgat ggcatttttg
     6601 ccgacgggta tgcttatcag cttgcaacct tgactgtaaa tcctgatacg ggtggatatg
     6661 attcatatct ggaagttacg gttgaaggac aaggcactac gggaataggc tggcaggaaa
     6721 acgtaaatgc acaacatttt tctttgcaac aaaattttcc aaatccggca accgagcaaa
     6781 caaccattcc ttttgtttta aaaacagctt cttccgtaca aatagaatta tacgacatca
     6841 acgcacaaaa aataaaaaca atagaccttg gcgatttgcc tccgggaaat catgaaagtc
     6901 aaattaattt cgaagcgttg tcattaccaa aagcgaatta catctatcaa ttggtagttc
     6961 aaaataatgc aggccgattt accgatcata aattaatgac ggtagcaaaa taattgctcg
     7021 aaaatattat tgcatattat aagcttgctg ttcgcccgcc atcaacaggt aaatttatac
     7081 cattgatata agcagcagca ggggaggata aaaatgcaac ggcgttggct atttcttccg
     7141 gtgcaccaaa tcttttcatt ggtatttctt ccagcatttt atgctccaca gcgtcaacgg
     7201 cattaccggt gcgcgcagca ttggcagaaa taataccggt caagcgttca gtttttgtag
     7261 ctccgggcag cacattgttt accgtaatac cgaaagctcc tagctcattg gcaagtgttt
     7321 tcgaccagtt agcaacagcg gcgcgaatgg tatttgatac cccgagattt ggcaaaggtg
     7381 ccttcactga agtactgatg atattgatga tccgcccata accactattt ttcatgcccg
     7441 gaatacaggc ggttgctaaa atatgattgc aaataagatg ttgcttaaag gcggctaaaa
     7501 attgctccgt ttccgcatca gaagcctttc ctgatgcagg tccgccggta ttgttcacca
     7561 gtatattgac tgttttttct cctgaggaaa aatatgcatg cattgcggcg cgcacctgct
     7621 cgggattgct gaaatcggca ataatggatt cgtgctctac gccatgtgga ttgggaagca
     7681 gttgttttac ctgattcaat ttatcggcat cgcgggctac caaaacaatg cgggcaccca
     7741 tcgatgccag ttcaaacgca caggctttgc caataccctg tgtgctgccg cataccatgg
     7801 cggtgtatgg atacagttgg atattcatga ttctgcgtaa atttaccacc taccaattca
     7861 ttaaagaaat aaacagataa atcatggcta ttgctggacc tttaaaccta aaacagtgga
     7921 tcgacgataa tcgccatctg ctcaaacctc cggtgggcaa tcaacagatc tataaaatga
     7981 acgaagactt catcgttatg gtggttggcg gaccaaactc gaggaaagat ttccactttg
     8041 aagatggtga agaattgttc tatcaattgg aaggcgatat tactgttgca attcaggaag
     8101 atggcaaacg cagagatata gttatcaaag aaggagaaat gtttttgctc ccaccaaatg
     8161 ttccgcacag tcccatgcga cctgcaaata ctgttggctt ggtgattgaa cgttatcgca
     8221 cggaaaagga atttgattcg tgtatgtggt tctgcgaaaa atgcaatcat aaattacatg
     8281 aggagacatt tccacttcag gatattgtaa atcaattacc gcaaatcatg ggtaaatttt
     8341 atgctgattt ggatctttgc acatgtaaat cttgcggaca tgtaatggaa ccaccgaagt
     8401 aaatctgaaa tttccggtgg ttgatgatta ttgtgaagtt gaaaaggaac acagattgaa
     8461 cagataaaaa cggattactt acactccgaa attacaaaac agatactcaa ggcattttat
     8521 actgtataca atgctttagg ttatgggttt ctagaaaaag tatacgaaaa cgcgctaaaa
     8581 attgagcttg agcaatcagg attacaggtt ttgcaacagg aatcaattag tgtattctat
     8641 gataatgcta aagttggtta ttatgtagct gatatgattg ttggaaagta cgatattgtt
     8701 gaaaataaag cctgcagcgc gcttaactca gaccacgaag cgcaattact aaactattta
     8761 aaagctacaa aaattgaggt tggcttatta ttaaatttcg gattaaaacc cgaattcaaa
     8821 ataaaaattt tcgaaaacaa gtataaataa atccgtccca atctgtttaa cctgttcaac
     8881 cagtgttcct tttcaaaaat tatgcttaaa atagacatcc acacccatat cattcccgag
     8941 catcttccga agtggagtga aaaatttctc gataacagat atatccattt agatcattat
     9001 tgtccgggtt gtgcgaaaat gatgcagggg gataaatttt ttcgtgaagt aaaaagtaat
     9061 tgttgggatg gcaatgtccg catagaagaa tgtgatgcaa caaatgtaaa tgttcaagtg
     9121 ctaagtgcaa ttccggtaat gtttcattat tgggaaaaga atgcggcata tgcacttgaa
     9181 acatcgcgtt ttcaaaacga ctttattgca gaggttgttt ctcgtcatcc tacgcgattc
     9241 atcggacttg gtacggtgcc tttacaagat ccgcaattga gtattgagga aatggaacgc
     9301 tgtgtaaaag agctcggact tgcaggaatt gaaattggct cgcatgtgaa cgactggaat
     9361 ttaaatgcag aagaattatt tccatttttc gaagctgcgg aaaaattagg tgctgcaatt
     9421 tttgttcatc catgggatat gatgggaaag gataaaatga ataaatactg gctcccatgg
     9481 ttggtaggta tgcctgccga aacatcactg gctatttgct caatgatatt cggtggtgta
     9541 ttcgaaaaat tgccaaattt aaaagtagca ttcgctcacg gcggcggcag ctttccttct
     9601 actatcggtc gcattgaaca tggatttaat gtaagacctg atttggtggc agttgataat
     9661 aaggtgaatc caagaacata tctcaataag ttttatctcg ataccctggt gcatgatgac
     9721 caaatgctga attatctcct cgatctgttc ggccccgcac aattagcact cggtagcgat
     9781 tatcccttcc cactcggcga acatcatccc ggtaaattga tagaaagcat gccttacgat
     9841 gattctatca aagcacgatt actgcatgga acggcattgg aatggctcgg acttgaaaaa
     9901 gggcaattcg agtaaattga aacatcgccg ttttctatag ctccgacaat tctgcctacc
     9961 tttgcggcat gtcagttaca tttaccgccg acaagcaatt ggctgcgcag ttagatgctg
    10021 cagacccgtt aaaggacttc aggcaacgct tttatatacc atcacaagat ggaaaagagc
    10081 agatttattt ttgtggtaac tcgctcggac tccaaccaaa atcaacacgc agttttattg
    10141 aacaggaatt gatcgattgg caacatcttg gtgttgaagg tcatttacat ggaaaaaatc
    10201 catggtttta ttatcatcat ttcctcgcag aaagcacggc aagaattgtt ggagcaaatc
    10261 cgcttgaagt tgttgtgatg aattcactca ccgtgaatct caatttattg atgataagtt
    10321 tttatcgccc gacaaaacag cgatataaaa tcatcatgga atacatggcc tttccaagcg
    10381 atcagtatgc tgtagaaaat caggttaaat ttcatggctt tgatcccttc gatgcaatta
    10441 ttgaactaat gccgcgcgag ggtgaagcag cgttgcgcac tgaagatatt ttggcaacca
    10501 ttgaacaaca taaagacagt attgcactta ttatgttggg tggtgtgaat tattatacgg
    10561 gtcagttatt cgatttgtca acaattactg catttacaaa aaaatgcagt agcgaaatag
    10621 ttgttggata cgatttggca catgccgccg gaaatgtaat gcttagttta catgattggg
    10681 gtgtagattt tgcaacatgg tgtagttata aatatttaaa tagcggtccg ggaggaacaa
    10741 gtggtgtttt tattcatgag aaacatgcgc aagacaattc tttgcctaga ttaagcggat
    10801 ggtggggtaa tgaagaaaat acgcgcttta aaatgcaaaa aggttttttc ccacagccgg
    10861 gtgcagcagg atggcaaatg agtaatgcac aaattctgcc tatggcagcc catcgagctt
    10921 cactcgaaat atttgatgca gccggaatgg acaacctggt acaaaaaagt aaattgctga
    10981 ccggatattt agaatattta ctgttgtccg gtgcacgcaa agattttaaa ataataacgc
    11041 ctgcagatca tgcacaacgc ggttgtcaat tatcaatagt catggagcat aatggaaaag
    11101 cggtattcga caaactgact gaaaatggca tcattgccga ttggcgagaa ccggatgtaa
    11161 tacgcgttgc tccggtgcca ttgtataata catttgaaga cgtatggcgt tttgcagata
    11221 tctttcattc ggcgttataa attattgacc tctttaaaac aaaaaagctc accaacttga
    11281 ttgatgagct tttttttatg acttcagctt attgaaccgg aggcatatac gccggtacta
    11341 cattgtctac cggaggaatg gatttcagat aagcataaat tgcctttaag tcattaacat
    11401 caaatttgcc aatttcctgc caaggcatgg gaggcataat ggggcgggta ttatccattc
    11461 ccatgtgctt tccttcctgt aaggctttta caaactgctc ttcactccac gagccgattc
    11521 cactttctga tggtgtgagg ttggcagcaa agctggttcc ccatggacca acggcgcatg
    11581 ttaatcctgg cgacaataat acccaagggc caactgtgtt tttgtcgtaa ggcgctacag
    11641 gttctcccga aggatgcccg ctaagcatgc gcgacatatc aggaaccggt ccttgaggtg
    11701 tcatgttttt gggtgtatgg caatcgttac atccgccaac agttaccata aattgtccac
    11761 gttgaacagg atcttctgcc tttacttccg gttccggaca gtcggcagat tttgtgcatg
    11821 cagacagggc aaatagtcct gcgcacacga tggttagggt aattcgagta tgcatgataa
    11881 ttggttgtta acctgaaagt taggtaaaat atttatgttg gtgaactctg ataaaaatca
    11941 cctgtcgtaa aaatcaaatc cttttacctt tgttacttcc acaagtaagc tatgcaagta
    12001 aaaaatattg ccattatggg tgcggggctg gtaggctcgc ttctgagtat gtatctcaaa
    12061 aaacatggtt atcaggtaac ggtgtacgaa cgtcgccccg attacaggaa ggctgaaaat
    12121 tatggcggac gatccatcaa cctggctata agtgaacgcg ggtggaaggg gcttgcaggg
    12181 gttggacttg aagaaatggt aagagagatt gcaataccga tgccggggcg catgattcat
    12241 gatgtgcaag ggaacttgaa cttccagcct tatggaaaac ccggggaagc aattaattcg
    12301 gtatcgcgag gcggtttgaa tgtgatgatg attgatgcag cagaaaaggc cggagttacc
    12361 tatttgttcg aacagcgtat tatggaaata gatccatcag gtaaaaaagc gatgatggaa
    12421 gatatgtcca ccggaagaca ttataccctg gaacccgact tgatcattgg tgctgatggg
    12481 gcctacagcc tcgtgcgcta tgccatgctt aaaacagatc gatttgatta tagtcaggct
    12541 tatgaagcac acgggtataa agagttgaca attcccgccg gaccgaatgg aagttttctg
    12601 atagaccgcg atgctttgca tatctggcca agaaagcagt ttatgctgat tgcactgcct
    12661 aatatcgatg gaagttttac ttgtaccttg ttcttttcgt tggaagggaa ggagtcgttt
    12721 gaggccatta aaacccttgc tgatcttgat gcctttttcg atacctattt ccctgatgta
    12781 aagtcattga tccccgacct tcaccacgat ttcttcgcaa accctaccgg attgctgatg
    12841 atcgttaaat gcttcccctg gagtcacggt aacaccatgc tgattggaga tgcggctcat
    12901 gccattatcc cattttatgg acagggaatg aattgcggtt tcgaagactg taccgtttta
    12961 gatacactga ttgaggaatt taaccacgat tggagcaaga tcatgccggc ctatgagcgc
    13021 ctacggaagc caaattccga tgccattgcc gacctcgcca aattaaattt cattgaaatg
    13081 cgtgatcttg tggcagatgc cgattttcaa ttcaagaagc ggatcgcagc caaggtgagt
    13141 gcgcttcacc ccgatcgttt cataccggtt tacagtatgg tgtcatttag tcacctacct
    13201 tacaaggatg ccctaagtga atatcatcgt caggatgcgg ttttaaccga aattttgggg
    13261 tggcctgaga ttgaaaatag gtgggaaacc gactaccttc cacagatcgc agagagactg
    13321 tttaataaat agtaaatatg taattgattg gttgtcaatg ttttaaatag accacctttc
    13381 gcgtctaaga cctttctcgt cttaaaccta acaggtctta aacttaacag gtttaagacc
    13441 tgttaggttt gatggattga tggcataaaa aaagcccctt ctttcgaaga ggctccttaa
    13501 atctatttac ttcaaaggat tagttaccgc cggtagctcc gccttttttc ttgatatcaa
    13561 tagtcttttt agtgtcgcca cctttcacgt taacatctac ttttttgtca ggagcaggtg
    13621 ttacagtagt ggtagatttt gttgaagtgc ttgatttgct tgatgttgag ctcttcatag
    13681 catccatttt catccactct tccatttcca tatgaagagc agcaattgaa tccattaaag
    13741 ctgttttttc agcagcacaa gcgctatcgc caacatgcat ttgtttttct aaatcagcga
    13801 ttttttgttc agcttctttg tctgaacagc tggcaagcac gaagcctccg gctaatacga
    13861 acaataaaaa tttgatcttc atatggtctg agtttaaaat ttggaatggt atttgactac
    13921 aaaggtatta ataatcaaac atcataccgt aattttgctc aactttttaa tcaaatcaag
    13981 atttatgaag actaaaatct ccgttttatc cctcgccgta atagctgccg gtggcatgtt
    14041 catcaccagc tgcggtactg aagacttgtc agttcctgta atcgttctcg aaggcgattc
    14101 tccttttatt gttgaacttg gcgataccta tactgatccg ggctttacag ctacagacga
    14161 tgaggatggc gatatcactg caagtgtaac agttgatgac agcgatgttg atactgaaga
    14221 aatcggcgaa tatgaagtta cttataccgt aactgatggc gccggaaacg taggcactga
    14281 aacgcgtatt gttcgtgttg ttatgggtaa agccgactac atgggaactt tccaagtgca
    14341 tgaaatctgc gacatggatg gtgatggcgt ttatggtgaa cccggcgttg actacgaaat
    14401 caacgactat accgtaactg ttacctccgg tggcgacgac aatgaagtgc ttttccaaaa
    14461 ctttggagct tatggcgcta ctgtaatcgt tcctgtttac ttcagcggcg atctgaatga
    14521 gatcctgact gtagacgatt acaacctgcc gggaactacc atctacttta acgctgatgg
    14581 cgaaatcact accggtacta cttccgacat tgaattcgac ctcgactaca atgctcagga
    14641 tggtgcaact atcatccctt gtcaggctac attcgtgaaa ttgtaatatc cacggaccgc
    14701 ttcatgcgga aataaaagac tcaaagcccc ggctgctaac cgctccgggg cttttgcatg
    14761 aatatcatta ccttagcctt tctgctatgc gtaaactgtt gccaatatct atcgtaatta
    14821 ttttctttac ctctgcctgc acgagggacg acagcactgc gcccctgatt cttatacagc
    14881 cggatgcgca tgcaccacat taccgaacaa tacccttcgt agaccccggt tgtgaagttg
    14941 ccgatgatta ttcgtgtgcc gatgccattg atttacaaat ttccaataat gtagacgtaa
    15001 ataaatatgg cacctataat atcatgtata ccgcaacgga tgaagccggt aatataaatg
    15061 aatacaatcg tccggttgat attattttgc ctatttccga ttattatgat ttcgcatatt
    15121 cggcatacga tacttgtacc tccggaaatt atttttatac cggattagct caagattgcg
    15181 actgtccgga tgataaagtt atcttcggaa atatcagcaa cttcggacca aatgcaactt
    15241 ttccgctccc cataaacggc caatataatc acatgattac cctcgacaca actaaattgg
    15301 cagtcacatt ttttggcaca ggtgttatga gtcctgctgc tgataccatt ttttgggaat
    15361 atgtaattat ggattccatt tctacggatg catgtcgttc ggtttggata aaatagatct
    15421 tttccgatca atttttgtta taacaacatg acaacattga atacaatttt cagccgcata
    15481 tttctcggta tattaattac cgtaagtcat tcggtgctcg ctgctgatcc cggtcgtatc
    15541 aatggtcaaa ttattgtgca attttattca aacgttgaac ctgaacgcgt aattatcgat
    15601 gttgagcttg catcaaaaac tgacgttgct ttattaaaac cgctttctgc atccttgaat
    15661 atctggttaa ttgccttcga tgaaaatgtg tataatgcag aattcgtaaa ggatgctttc
    15721 tttaaaaatc cggatgtggc attggtgcaa ttcaatcata cattttctga gcgcagcatt
    15781 cccgatgatc cttttttcta tcagcaatgg ggcctgctaa atgatggtgc agggggaatt
    15841 gcagatgcag atattgatgc cgatcttgca tgggacatca caaccggcgg cgttacggca
    15901 aaaggtgata ctatagttgt tgcactgatc agcgaagggc aattttatgc acatgaagat
    15961 ctcgattatt ttatcaatag aaatgaaatt ccggataatg atattgatga tgacgccaat
    16021 ggttatatcg atgattatca cggctggaat ggcactactc aaaccgatag cgttcccgaa
    16081 agattgcatg gcactcacgt agccggcatt gcaggtgcaa taggaaataa tggattaggt
    16141 atttcgggtg tttctcagaa gctgaaaata ttacctgtat ataaccttac tgttgaagca
    16201 gatgctgtgg cgtcttatgc atacgcccgc gacatgcgaa aaatttataa cgaaacaaat
    16261 ggcgaaaaag gcgcttatat tgtcgcaaca aattcttcgt tcggtgtaga tttcggaaat
    16321 ccggatgatt accctatttg gtgtgctatg ttcgatagtc ttggtgttgt tggtgtatta
    16381 agtgtagctt caactgctaa tacgtcggtg aatgtcgata atgtatacga catacctact
    16441 gcttgtccgt ctgaatatct gatcacagta accatgactg ataaagcaga taacttattt
    16501 tcggcagcgt atggtgcaaa aagtattgat cttggtgctc cggggaattt gatctattca
    16561 acaattattg ataattccta tggcatccta agtggaacat caatgtctgc accacatgta
    16621 acaggtgcta ttgctatgat gtatgcagcg gcctgccctg agttttttga tgcacttgag
    16681 gataacaatg aattgatgct gctcaaatta aaacaatatc tgctcgaagg cgtggatgtt
    16741 gttccggatc ttgaaggtgt tacggtaagc aatggaagat taaatgtttt tcgcgctata
    16801 gaaaatttaa ccgtgagtgg tttttgtgca accggttttg cggaaaatgc actgggtgta
    16861 gcctacccaa atccggcaca ggaattatta tttattaacg gcgcaacaat tattgatcag
    16921 gaagtgcaag tgcaagttgt aaatacactc ggacaagtgg tttaccagcg taccggtgat
    16981 cgctcaccat ttatattttc aggaatccct gtcggcgatc tgccttcggg gtattatgct
    17041 gtttctgtat acaacccgga ttctgattta ttattcgggt cgggtgttat aattcaacat
    17101 caataaattt tattttcgtg ctatggacaa acgatgggtg attaaagtga cggaggagga
    17161 aaaagttcag tccctgcaac aggagttgaa aatacatcct gtaatctgca atttgttggt
    17221 gcaacgtggc attacaacat atcaggaagc aaaacagttt tttagacctt cattagatga
    17281 tctgcatgat ccttttttaa tgagggatat ggagaaggca gtaaaacgca ttactgaggc
    17341 aattaataat caggagcgca ttctcatttt tggagattat gatgttgatg gcaccactgc
    17401 tgttgcgttg atgtatgctt ttttttattc gcgatatcgc aatatagatt tttatattcc
    17461 ggatagatat aaggaaggat acggtatttc ctttcaggga atagattacg caaaagaaca
    17521 tcagtgcacg ctcatgattg cacttgattg cgggataaaa gctaatgaca aggcagcata
    17581 tgcaaaagaa ttaggcatag acctcatcat atgcgatcac cacctccccg gagatattct
    17641 ccctgatgcc tatgcgttgt tagatccgca tagaccggat tgttcctatc catataaaga
    17701 tttaagtgga tgtggtatcg gatttaaagt ggttcatgca tttgcaaaat atcacgacat
    17761 ccccgttgaa caagttattt cgtacctcga ccttgtagtt gtttcaattg ccagtgatat
    17821 tgttcccata atgggtgaaa atcgcatact cgcttactat ggtcttgaac aattaaataa
    17881 aaaccctcgt cccggtttaa aagcacttat cgatatcacc ggttatacgc caccgttaaa
    17941 tatttcaaat attgtgttcg gacttggtcc tcgaattaat gctgccggca gaatggatga
    18001 tgccaatcaa gcagtgaaaa tgcttatttc cgaaaatgta gacgctgcaa aaaatggtgc
    18061 cgatgtatta caagaaaaaa atgtcgacag aaaagaagta gataaaatga tcactgccga
    18121 cgcgttagat attttggcaa cagatccttt gacaccgggt agagtatcaa ctgtgttgcg
    18181 cagcagtaat tggcataaag gtgtcattgg tattgtagca tcgcgtttgc ttgatcacta
    18241 ttacagacca accatcatgc ttgccgaaaa agacggcatg ctcttcggaa gtgcgcgttc
    18301 ggtaaagaat ttcgacatct acgaagcaat taaagcttgc agcgatctgt tagaacaatt
    18361 tggcggacat atgtatgcag cagggctttc tatgcgacag gaaaactttg aagctttccg
    18421 cgataaattt gaccatgtag tgcgcgaacg cattcgtcct gaacaattaa ttccggagat
    18481 agaaattgat tctaccttgg agcttaaaga tattacgccg tcatttttta atatagttcg
    18541 tcagtttgct ccttttggtc cggggaatat ggagcccgtt tttatcagcg agcaattgat
    18601 gaataccggt agaagcaaaa tagttggcga tggtcacctc aaacttgccg ttcgcgacag
    18661 caggcgtttc accgcagacg gcatcgcctt tcaaaagggt catcattttc ctttaatagc
    18721 tgacggaaaa aggttcgata tttgctatac cattgaagaa aatacctttc gtgatatgac
    18781 aaacatacaa ttgaatgttc gtgatattaa ggtaaatgtg taggcatcat tactgaatgt
    18841 aaatattgca aagcatgatc gtaagagcag aaaaattaat taagaaatat aaacaacgca
    18901 cagtagtaaa tgaggtaagc gttgaagtaa atcaaggaga gattgtagga cttctcggac
    18961 ccaatggcgc cggtaaaaca acttcgtttt acatgatcgt tggattgata cgtccgcttt
    19021 caggaaaagt ttttctggat aacatggata tcacctcttt tccgatgtat aaacgtgcac
    19081 agcttggttt gggatatctt cctcaggaag caagtgtttt tagaaaaatg agtgtagaga
    19141 ataatattgc cagtgtattg caaatgacca acctctcgca taaagagcaa aaagcaaaac
    19201 ttgaatcttt gcttgatgaa ttcggcttaa aacatgttcg caagagtaat ggtgatgtgt
    19261 taagtggagg cgaacgaaga agaaccgaaa ttgccagagc attggctacc gatccgaagt
    19321 ttatattgct ggatgagcct tttgccggta tcgatccgat agcagttgag gacattcagc
    19381 ttattgttga aaaattgaag ttcaaaaata tcggagtgct cattacagac cataatgtgc
    19441 aggaaaccct ctcaattact gatagggcat atttactttt tgagggtaaa attttgaaac
    19501 agggcacggc agaagacctg gcaggcgacc cacaagtaag aaaagtttac ttagggcaga
    19561 atttcgttct gcggaaaagt cgcgctacaa cgaacactac ccaacctcct gtggaataat
    19621 ctataccttt attctgtaag ccaccgtatt tttgcgaagc gaatgccaat tatccacgcc
    19681 atagcctctc gtgtttttct ccgtaatgtg gagagaatcc actcagtggt gtcggaccct
    19741 attcgtgctc aagatcaggt atttcaaacc ctgatcgatg gcggccgaga taccgtgtgg
    19801 ggtaaacaat acgattatgc ctcgttgcga tcgctaaacg acctgaaaaa caggtttccc
    19861 gtgcaggatt atgacacttt aaagccttac atagaccgta tcatgcaagg cgaacaacag
    19921 gtgctgtggc atagtcatat tgcctggttt gcaaaatcct cgggcaccac cagcgataaa
    19981 agcaagttta ttcccgttag cagcatggct ttggaagaat gtcattttcg cggtggcaga
    20041 gatgttatag ccatgtacct gcataataat ccggaatcaa aactttttga tggtaagtcg
    20101 ttgttgatgg ggggcagtca tcaaatcaac aaactcaatc agcattcgaa atacggcgat
    20161 gtaagtgcgg tgatgatgca aaatttgcca tgggctgcaa aatatgccat gactccatcg
    20221 cttgagattg cactaatgga tgagtgggaa agcaagatcg aagccatggc gcattacaca
    20281 aaaaatgata atgtgacatc cattgccggt gtgcctacat ggacaatagt attgataagg
    20341 agattgtttg aattgtgtgg caccgataat gtcaaagaca tttggccaaa tctggaatta
    20401 tatattcatg gtggcgtaag ttttaaaccg tatcgcagtc agttcaatac ccttattaga
    20461 aaggataaca tgcattatgt tgaaacatat aatgcgagcg aaggattttt tgcatttcaa
    20521 gacagacttg gtgcagatga catgttgttg atgccggatt atggtctgtt ttatgaattc
    20581 attccaatgg aagaattcga caaagaagac ccgaaaacac tgagttgggg tgaagtggaa
    20641 ttaggcaaaa attatgcagt agttgtatcc tcaaatgccg gtttgtggcg atataaaatc
    20701 ggcgatacca ttcggtttac ttcgttatat ccacatagag tgcaggtttc agggcgtgtg
    20761 aaacatttta ttaatgcctt cggcgaagag gtaatagttg ataacagcga taaagctatt
    20821 gcagaagcaa gtgcccaaac cggagcaaaa gttgtagaat atactgcggc acccatttat
    20881 atttcaggca atgaaaaagg tggtcacgaa tggattatcg aattcagtac ccctcccgaa
    20941 aatatagaac aatttacggc catcctggat gcaacactta agtcgatcaa tagtgattat
    21001 gaggcaaaac gtcataagga cctcgcactc aaaatgccgg tagtacatat tgccagaaaa
    21061 ggtgcttttt atgattggtt gaaatcaaaa ggcaagcttg gcggacagca caaaatacca
    21121 cgcctaagca acgagcgtac ctatgtggat gaaatattga aattcctctg atcgcttcat
    21181 cgccgttagg caattcctct taatattttt gctaactttg ataaggtcct atgactttaa
    21241 acagcacttc atgcaacgat atacagctat actggtagac gacgaggaac acggtcgccg
    21301 gaaccttcaa acattactcg aaaaatactg tcccgaaata gaagtaatcg ccgaagctgc
    21361 cggtgctgcc gaagcgaaag aaaaaattct gtccttaact ccacaagttt tattcctcga
    21421 tatcaatatg cctgaactca atggctttga tttgttggaa tcgattgcaa aaaaagattt
    21481 cgcggtggta tttgtaacag cacatcgcga ttttggtatt gaagcagtta aagccggtgc
    21541 caccgattat ctgctcaaac ctattgtaat aaaagaattg caacaggcgg ttaaaaaagt
    21601 ggtcatgtat tacgacgagc gcagtgcgga agcaaataat gccgatgttc ctaaacccga
    21661 acgtatcact ttaggacata ccggcggttt tagcgtagtg gaaatgaaag atatcgttcg
    21721 gttggaggcg gaaagcaatt acacccgcgt tcatgtgaca ggaaaaaaat cttattttgt
    21781 atctaagccg ttgaaggttt ttgaagatag cctcaaggat aatattttct tccgtgtaca
    21841 tagatcttac attatcaatc ttaatcatgt aaaagaattt ttgcgtgaag acggaggcgt
    21901 aattgttatg cttgatgatg cacgaattca gttgccaaag gcgcgatata acgacttttt
    21961 ggaggcaatg cgcaaattgt ctattgctat ctaattcctc attcatgact ttgaaatcat
    22021 ttatttgctt agtatttatt tgtattggca tgcatgtcaa agcgcagata tttcctgcaa
    22081 ggcaatatac tacaagagat gaattggcaa acagtaatgt gtatgatatc tgtcatgatg
    22141 atcgtggata tttatggttt gccacagagc gcggagtaag tcgttttgat ggatacaaat
    22201 tcaccaatta tctatttaag gaggggcttg atggtgcaat tgtatttagc ctttcaaagg
    22261 atgcgagggg taatattctt gctgcaacaa agtttgacgg ggcatttaaa atgtcaggaa
    22321 acttcaataa gatcattgaa gaagatttgt tacttgccaa tgaacaaaca gtattggcgg
    22381 gtaaatattt ttattcatta aaagatggtg tacgggtagc cgtatatcaa atgggtaata
    22441 acaaaacttc ggtattgtcg tttcctggcg gtgctaagcc atatttcatt ttgcagaaag
    22501 atagcagtgc agtatatata ggcacctcaa gcggcgtttt tatttctaag aatggtaaaa
    22561 ctccggagcg tgtattccct gaaattaatg agccggtcca tagcctggcc attcgaggaa
    22621 atacggtttt tgttggtttg caaggcgcgg tttatgcgat tgctgacaat gctgtttcag
    22681 tggcggcgtt agtaccggat ggtggtaaag tgaaacggtt gatggttgac gacaacaaca
    22741 gaatttggtg cgccactttt cctgaaaatc gattgtatat gttcgacaag aacacggcga
    22801 ttgatatttc tcagaaatta aatattgcag gtatctcggt caataagata ttggaagaca
    22861 atgaaggaaa tgtttggatt gcaacctatg gaaagggagt attttgtttg catcatatgt
    22921 attgtactaa ctacacgtcg tatgatggtt taaaaaatga atatatcact tcgctattgc
    22981 caattgaaca tgaattattg attggtacat ataacggttt gtttggaaat actaacaatc
    23041 aaatcacacc actaaaatca tttaacaatg aattagaatt cattcgcaag cttgatatac
    23101 atgatgatct gattattgct accgtttcag gtatttctgc tgataaagta cttatcaagc
    23161 gatcaacaat cggcaatcac cctttgcaat atgtgcatgc aactaccact tttattgatg
    23221 gcaattatat ttattatagt cgctgggata aaaaagtgtg gagagcgcaa ataaatggga
    23281 ctgaaatagg tgatgcagag gttgtgttcg aagacaagga gggcgagtgg acacgagtga
    23341 acgcaatttt ccgcgacccc caaaataaac tgtgggttgg tactacaaga ggtgtttaca
    23401 ttatttcagc gaataattct tgggtaagga atgataccgg gtttttacgc accaatatca
    23461 ccgcctttgc tttggatcat gatggatctg ttttgcttgg aacagacaaa gggctcgtca
    23521 ggtataacga tggtaaatgg attgcttctc atgaaataaa tggtaagaac cttgaaaata
    23581 taacaggtct agctcatgat aataacgatc gcatttggat aagtacatta aatggcttgt
    23641 ttttaatggc tgaaaatgaa cttattcagt ttgatgccag aaataccttg ttgagcgacg
    23701 agattaatgc tttagcctac gatgcttcag agaatgccat ctgggtgggc acaagttttg
    23761 ggttgtcgcg tattgatgtt ggattattcg acaaagctgt tgtaattgca ccggcggcca
    23821 tttttaaaac attgcgagca gccgactcga tttatcgcga tcttggtatt aatggcaggt
    23881 tggagttacc ctatacttca aaaaatttga gcgttcgatt ttctgccatt cagtttgcag
    23941 ctccggaagg gataaaattc ttttataaat tcgatgatgg tccctgggag ccgactaccg
    24001 gtcgtcagat tgaatttgcc agcattccat atggtgaaca tactattcaa ttaaaatcgg
    24061 ttggagaacg tggagttgaa gggcctgtag cgaacctgac cattttggta accaccccaa
    24121 tctgggcgac cgcctggttt aaattgctta tcggccttac tattgcaggc atagcatatt
    24181 tccttttgcg aaaacgattt gaaatacaac gcaaaaagca acaagagcga ctcgaattgc
    24241 aaagtaaagt tgctgagctg cgtcaccagg ctctggcagc tagtatgaac ccgcatttta
    24301 tttttaatgc actcaattcc attcaacatt ttatcaatag ccataatact gaggaagcta
    24361 ccgattatct cggcaagttt gcgcgattga ttcgcatgat gctggattat ggtggtcgca
    24421 catttattcc gctcaaggat gagctggagc gattagaata ttaccttgaa ttagaaaaag
    24481 tgcgatttgg cgaaaagttg aattattcca ttgaagtgga tgacagtttg cgaaataacc
    24541 cactcgaaat accaaatatg gtcatacaac cggttgttga aaatgctctt tggcatggat
    24601 tgttgccggc aaataggaat ggtcatttgc tggtcaaatt cgcgcaacgc gataacgtca
    24661 tctttgtaac agttgatgat gacggtatcg gaataataga aagcaagcga agaaaaaagt
    24721 catcacataa ctccttgggt atccaaatga ttcgtgagcg acttgaacta ttgaaccgct
    24781 tgagtggata catcgcagaa attgaaataa aggataaatc ggagatcaac gccgataaag
    24841 aaggaacaat tgcccagatc gtcatgggag ggaaaaaaga acaacaataa atattgttcc
    24901 aattacggtc gattgatcgc aggaacctat atgacattat tgcttaagca gttttggtta
    24961 cattaattta acaatgctac ctttgtccac tcttattaat tacattaata gctttcatga
    25021 aatcaatcta ctcaggctac ttgttattgg ctgccattac gatagttaca ttatttgatg
    25081 cttgccggcc ggtacaacct gatcaacgac atcagataac ggtcttacat tagatatatc
    25141 acccgagggt atgcaaataa tggaagccaa cagaacaatt gcgctccaaa acggcgtttt
    25201 gttatcaggt gataacgatt gggttggtgc catactccac acaagcgata ctttttacaa
    25261 ttgcaatgtc aggttaaaag gtgattgggt tgatcatttg caaggagata aatggtcgtt
    25321 tagaataaag ctcggaagcg atgacgccat taatggtatg cgcgtatttt ctgttcagga
    25381 tccaattaca agagatcatt tatctgaata tgtttatcat caatggcttg ctgctgaaga
    25441 cattttaact actagatatg atttcatgcc atttacgttg aacaagggta agacatatac
    25501 ttacgcagtt gaggaacatt ttgatatcaa cctgatcgaa tctcaacacc gaagggccgg
    25561 tccgatattg agattttcag aagagttttt atgggatcta agagtacaag atagtgcagc
    25621 tttcaaatcc ggcgggatgt acgaaaataa tattgatgta tttgctatca ccgatattct
    25681 ttcatttagt gataagggat tggggaaaga tgttgagctt accaaacaat acgaagtggc
    25741 gagaacatta atgcaacaat atcgcgcagg gaaggtagca ctggccgaca tttttgatat
    25801 ggataaaatg gcaaaatatt tcgcaattgt tgacattaac caggcttacc atagtttaat
    25861 atggcataat atgcgatttt attttaatcc gataactcaa aaactcgaac ctattggatt
    25921 tgatggcttt acagagtatg gcgtttatga caatatcaaa cgcccgttta tcggatattc
    25981 cgcttcttat caataccgtc agaaaattga agatgatgct ttaaccgata gatttttcaa
    26041 cgacttcgaa ttttcaaata cttacgcaaa atacctgcat aaatttgcgg atactaatta
    26101 tataaacgct ttttttgatt caatcggcga atcgttgact gaaaaaggca acttaattcg
    26161 aaaggattat cccgaatacc aatacaacaa gaaacatatt tatgaccgta ctctggcgat
    26221 tgagattaac ctcaagcctc agggagatta tacgttgcgc gcatttgcag atatggcggc
    26281 acccgctaaa attggaataa taaatcatgt tgctgttcct gtatcagttt tgggcatgag
    26341 tataagacct gataccatca tgtattatct tgataaacca ataaatcttc ccgcctggaa
    26401 tgaatataaa gcaccgcctt ttgccgcagt gaaaaaaatt ggagaaagtc aatacttgtt
    26461 ttacaaagtt gcgggaacag atagtctggt gtcagttaaa atttctccat gggaagatga
    26521 ccttgttcaa acgcctgagc aaagactatt taatacaaca atttcttcca atgaattgta
    26581 tgtaattaaa aataatgaaa tattctttaa accgggatta catgtagtta cgaagcctat
    26641 aatctttccg gcaggttata atattcatat ttcacccgga acacaattgg acctgcgcaa
    26701 tggagcaatg ttcgtttcaa gatcgccgtt gagcgttatc ggtacagaat catctccaat
    26761 taaaatattt tcatcagatt ctacctccat gggatttact gttttgcagg ctgcttcagc
    26821 atccgcattt caatatgtga tttttgatca tttatccgct ttggatcatg aaggttggaa
    26881 attaatggga gcagttaatt tttacgaatc aaccgtatca ttggagcatt gccgatttac
    26941 gcatagccaa tgtgaagatg ccttgaatat catgcgttca aattttagca tcgactattc
    27001 ctattttgca aatacctatg gcgatgcgtt tgattctgat tttagtacag gcacagtaaa
    27061 aaacacttac ctcacacata ctggtaatga tgcgcttgat ttttcaggta gttttattga
    27121 agttaaaaga tgtgaattag tccatctcgg cgataaaggt atgagctgcg gcgaggactc
    27181 aaaaattaca atatcggact gttctatcaa ggatgcacgg ataggagtag cagccaagga
    27241 tttaagtaga gtagaagtac aaaacctgca gatctcagat tcaggcatcg gatatctggc
    27301 ttttgttaaa aaacaagcct ttggaaaatc ctctattcag gtgactggag gcacgctcac
    27361 caatgtaaag caaaccactt cagtgctcga cggctgtgaa attatcatgc aataggccgg
    27421 ttttagctga tttcagccat ttcaggtcac tctaagcgga ccggaaatgc cctttggggg
    27481 gcgctttcaa cacgatttgc acatttcggt gcaatgccct atctttgcac cactcagaag
    27541 aggggacacc aaaaagtccc ctcttttgtt tctgtaacca tgggtaaccg tgagcaaata
    27601 gaagaatggc tggggcctaa gctggaggag ctgggttgtt ttttggtgga tatccgcatc
    27661 aatccatcgt catcaaagta tgaggtatat attgataccc caacaggtgt tacgattgaa
    27721 caatgcgaaa aggttagtcg ccacctctcc ttcatgatag agaaccaggg aggctttccc
    27781 gaaaaatata cacttgatgt gtcgagtccg ggaatggata accctttcaa ggtgcagcag
    27841 caatacgaca aaaccattgg caaaacggtt gaagtgcttc tgctttccgg cgtgaaaatg
    27901 gaaggcatgc ttaagaaagc agatggtaaa gtgctgcatt tggaagtgca tcatccgcca
    27961 aagaaaaaag gcatgaaacc ggagataact ttagaaacct atgatttcag cgagataaaa
    28021 cacgtgaaaa agaaaatcaa tttttaactc aaataaacag caacaccgct tcatccggga
    28081 ttttccggac gaggcattaa aaatcaagtg ctatgaacag catagaacta gtagaatcct
    28141 tctcggagtt taaagacgtc aaaaacattg accgtcctac attgatgaaa gtgttggaag
    28201 acatcttccg taccttactt cgtaaaaagt atgaaagcga tgagaacttt gacatcatcg
    28261 taaatacaga aaaaggtgac ctcgagatct ggcatcgccg cgagatcgtt gaggatggaa
    28321 tggttgagaa tcctgttaca caaatcagcc tcgccgatgc cctgaaaata gagcccgact
    28381 tttcggtagg tgaagaaacc tatgaagagg tgagcatcga aagcttcggt cgccgcgcta
    28441 tcctggctgc acgtcaggct ttggttcagc gtatcatgga gcttgaaaaa gacgaagttt
    28501 acaagaaata taaagatcgt gaaggtgaga tcatcaccgg cgaagtttat caggtatgga
    28561 aaaaagaaat cctcctgctc gacgacgatg gcaatgagtt aatacttccc aaaggtgaac
    28621 taattcattc cgacaactat aaaaaaggtg atgctatacg cggtgttgtg aagaaagttg
    28681 acttccgcaa taacaatccg gtagttattg tttctcgtac cgatccaaaa ttcctcgaaa
    28741 aattactcga acaggaagta cctgaagtat ttgatggatt gatcgtgata cgccgcatcg
    28801 ttcgtgctcc cggtgagcgt gcgaaagttg ctgttgagtc atacgacgat cgcatcgatc
    28861 cggttggcgc ttgcgttggt atgaaaggat ctcgtattca tggtatcgtt cgtgaattgc
    28921 gcaacgaaaa cattgatatt atcaactgga ccaacaacaa ccagttgctc atccagcgcg
    28981 ctttaagccc tgcaaaaatt accagtattg aactggataa tgatcgcaaa aaggctgctg
    29041 tttacctcgc gcaagatcag gtttccctgg ccatcggaaa gggtggattg aatattaaat
    29101 tagcttgttc actcaccggt tacgacatcg atgtcttccg cgataatgaa gaagcagacg
    29161 tagatatcga cctcgacgaa tttgcagatg agatcgacga atggatcata gatgagctca
    29221 aggctatcgg ttgcgatact gccaagagcg ttctggaact gagcgttgat gaactggttc
    29281 gccgcaccga ccttgaggaa gaaaccatta aagaaatagt tagaatatta aaagcagagt
    29341 tcgacgaaga ttaagattcc acagggtgct gtaaaagcat cacgcgataa tcctatcttt
    29401 gtagccacaa tactgaatgt cagaagaaaa atccatacga ttagcaaagg ccgcgagcga
    29461 gtttaacgta tctcttgccc atattgtcga cctgctgaaa accaaaggtt tctcagtgga
    29521 gccgaaacct acttccaaaa tttcggaaga gatgcacaat atcctgctca aggaatttgg
    29581 caaagacaaa gacctgaagg ggaaagccga tcaattgaat atcggtatcg gccgcaaact
    29641 ggaaaaagag aaacaagaga aagaagaagc agaagccctg caggaaaaac ctgtacaaga
    29701 ggtagttgtt gaagaagaaa aagttgttgc tcctaaagtt accaaggaaa aaaaggttga
    29761 ggaagaagtg aaagctcctg agcctgaacc cgaaccggaa ccggaacccg aagctcccgc
    29821 aaaggaagag aaagatgagt tgataaaaat ggagcgcggc aaagttggct taacggttgt
    29881 agataaaata gatctcaaca agaaaaaagc gccgaaaaaa actgtagctc ccgaagaaaa
    29941 accggtagtt gaaaaagcta aatctgacaa aaaagaaaaa gtagaagagg taaaacctgt
    30001 tgtagaaaaa caggaaatac cggttgcaaa acaggtaacg gaagttcctg aaaagaaacc
    30061 ggaagcagaa atgatcgaga ctaaacgcgt tacgctggaa ggtcctaaga tcattggtcg
    30121 cattgaaatc aaagaagaaa agaaacctga aaaacgcgga cctgataata atgcggaaaa
    30181 gaaaaaacgc aaacgcatta ttaagcatga acgcatcaac gttgataagg taaaagaaga
    30241 agaacgtcaa cgcgaaaaca gaaacagaca tcagggtggt caaggaacca gaaaacctga
    30301 agtagattct gaagaagcac aacgtcagat acaggaacaa attcgtcaaa cacttgccaa
    30361 acttaccggc ggcggtcaac aaaaaggtcg cggttcgaga atggcaaagc aaatgaaacg
    30421 cgagcgtcgt gaagctgcag aaattgaagc agaagcagcg tcgcacagca atcagttaat
    30481 ggttgccgaa tttacttcgc tttcagaatt ggcgagtctt atgaatgtat cgccaacttc
    30541 actagtgcaa agctgtttca acctcggtat gatggtttcc atcaatcagc gtcttgatgc
    30601 agagatcata gaattgttgg ccgaagaaaa tggattcaag gttcaattta tcaatgccac
    30661 cgaagaggaa acagaagaag acgatattcc ggatgatcct gcagaccttg agacaagacc
    30721 accgatagtt accatcatgg gtcacgttga ccatggtaaa acatcgttgc tcgattatat
    30781 ccgcgaaaca aatgtagttg ccggtgagaa gggtggtatc actcagcaca tcggtgctta
    30841 cgaagtagac ctgccaaatg gcaaacgcat cgcattcctc gatacgccgg gtcacgaagc
    30901 ctttaccgcc atgcgtgctc gcggagcgaa acttactgat attgcagtta ttgtaattgc
    30961 tgccgatgac caggtgatgc ctcaaacaaa agaagccatc agtcactcgc aagcagcaaa
    31021 cgtaccaatg atcttcgcga tcaataagat tgataaaccg ggtgctaatt ccgataaaat
    31081 aaaggaacaa ctcagccaga tgaatattct ggttgaagat tgggggggga aattccaaag
    31141 tcaggagata tctgcaaaat caggtcttaa tgttgaaaca cttctggaaa aaattctcct
    31201 tgaagcagaa attctcgacc tgaaagctaa tcctgataaa gaagctgtag gtaccgttat
    31261 tgaagcatca ttagataaag gacgcggata tgtatccaat atcatggtgc aggaaggaac
    31321 actgaaagtt ggtgatatga tggttgccgg tccattcttt ggaaaggtaa aagccatgtt
    31381 caacgaacga aatcaacgca tcactgaagc aggtccttca acaccattgc tggtattagg
    31441 tttgaatggt gctcctcaag cgggtgagaa attcaaaata tttgaagacg aaacccaggc
    31501 aaaatctgta gccaacaaac gcagtcagat cttgcgcgaa cagggtatca gaaccaaaaa
    31561 acatatcacc ctcgaagaga tcggtcgccg tagggcattg ggtaccttca gagaattgaa
    31621 tatcatcatc aaggccgacg ttgacggttc tacagaggcg cttaccgaca gcttacaaaa
    31681 actgtctacc aaagaggtac aggttaacgt tatcttcaaa ggtgttggtc agatcacaga
    31741 aagcgacatc atgctcgcat ctgcttccga tgctctgatc gttggcttcc aggtacgtcc
    31801 aagcagctct gtccgcaaac tggctgagaa agaaggtgtg gagatcagac tttacagcat
    31861 tatctacaac gctattgaag aagtgaaagc cgccatggaa ggcatgcttg caccgaaaat
    31921 ggaagaaaag gttatttgta tggtcgaagt gcgcgatact ttcaaaattt cgaaagtcgg
    31981 cactattgca ggctgttacg ttaccgaagg caagatcacc agaaatacca aaattcgact
    32041 tgtacgcgat ggtatcgtaa ttcataccgg ttcattgagc tcactgaaac gcttcaaaga
    32101 tgacgcgaag gaagttacaa ccagcatgga gtgcggtctt accatcaaca actacaacga
    32161 tattcagccg ggcgatatga tcgagggtta tgaagaggtt gaagtgaaac gttcactgta
    32221 atctgctact agggaaccaa agttcggttc attctgcata cgaaataaaa aaaggcgaca
    32281 tggagcagtc gcctttttca tgatggatac catcatcttt tagggaatca tcaacccgaa
    32341 tcagatcccg aaaaaatttg tgttttcacg aagtgctttc atggggtgat agcacttgtg
    32401 tttacgggat agtgttttca gtaggatcga ggtcaaattg acggcatttt ggccttcaca
    32461 acaacccact ttgagtgaaa tgacctaact attgagtgaa atgtgctatc tattgagtga
    32521 atgagggcga ttttttaggc gaaatgacac cttttcgtga tgaaatgtgt cggtttgaat
    32581 gttgaataag cttgctgata gttaaaattg cctgtaatct gcatcgtagc tcctgcggtt
    32641 gatgagaatt gctttatgca ggatttggta ttcatctttt gtgtaccttt gcattctaat
    32701 caagtataga tgagcaacgt attattgctt tcaatgcccg gcggaatgga atgggtattt
    32761 atcatattgg ctgttttgct tctgtttggc ggcaaaaaaa ttccggaact gatgcgtgga
    32821 cttggaaaag gtatccgcga gttcaatgat gccaaaaaca acgtaagtcg cgagattcgt
    32881 gagggtatga atgaagaacc taaaaaatct gaagagaagt aattactgtc tttccagatg
    32941 tctgctgttt cggattcttt gctgcgttcc ggtttacacg ccgtacaggc tgacctgcgt
    33001 gcgggtacaa tttctgtgcg tcagatcgta gaacactatc tccgggtaaa cagagacaaa
    33061 cagcatctca acgcttttct tgaaattttt gaacacgatg cactgcacca ggcagatgag
    33121 atcgatgcac gtgtgcgctc cggcaagccg atgggtaagt tgttcggaat ggttatcggt
    33181 gttaaagatg taatatccat aaagggtaag cgtctcaccg catcatcaaa ggtactcgac
    33241 gattttgagt cattgtataa tgctacagtt atcgatcgat tgcgacatgc tgatgccatc
    33301 attcttggtc gctgcaactg cgatgagttt gccatgggtt ccagtaatga aaacagtgct
    33361 tttggcccgg tattaaacga tcttgataac acacgtgttt ccggtggcag ctccggaggt
    33421 tcagctgtgg cagttcaagc cggactttgt atggcaactt tgggctctga aacgggaggg
    33481 agtatccgtc aacccgcatc ctttaccggg actatcggtt ttaaacccac ttatggtcgc
    33541 ctctcacgat atggtttgat tgcatttgct tcttcatttg atcagattgg tccaatcacc
    33601 aatactgttg aggatatggc attgatgatg gaggctatgg caggacagga tccgcatgac
    33661 gccacttcat ctcccgaacc ggtatcacat tattcagagc acctggatgc acctaaaaag
    33721 ctgcgtattg cctattaccc gcaaaccctt gataatccta agatagaccc tgagatcagc
    33781 gctgcaatgt atggattgat cgacaaattg cgtgctgatg gtcatgtggt ggagtcctgt
    33841 gacctgaaat atctcgacta tctggttccg acctattatg tgcttactac ggcagaagca
    33901 tcatcaaacc tcagcagatt ttctggcatt cattacgggt ttcgtgacat ggaagcggag
    33961 gatatggaga gcgtgattcg ccgttccaga tcgaagggtt ttggcaaaga agtgcagcga
    34021 agaatcatgc tcggcacctt tgttttgagc tcaggatatt acgatgctta ttacaccagg
    34081 gcacaaaaag ttcgacgaat aattcgcgat gatacggcag aaacactttc gtcgtacgac
    34141 cttattttac tgcctaccac gcccactacg gcgtttaaac taggtgagaa aacaaccgat
    34201 cctatcgaga tgtacctcgc cgatattttt accgttcaag ccaacattac aggcctgcct
    34261 gccatctctg tccctttatt tagacatagc aacggaatgc ccttcggagc ccagttaatg
    34321 ggcaattata acagtgaaga actgctcttt tctgtgacat cgtaccttct taaaaattat
    34381 taaaatttag gtgtggatat tgatttatcc gctttctgac gcaataaaac ggtaatttta
    34441 tagttttata gccctgaaac cattgcctaa accgagaaaa acgagggaat aacgaatgca
    34501 ctttttgaga attaccgtat gctgcctttt gctggggggt atagcaaaag cacaggtctc
    34561 taattctcca actctcaacc cttctgatga gagtgtggat catcttgaca gtctgctcga
    34621 ggcgttttac gatagtaatc cggcctggag gtttgatgaa caggattatg ctttgaatgg
    34681 cttagccgtt acagatacac cgcgatttac tcctgaagtg attgctgatc gcatcagcaa
    34741 gatcaattcc cccattccat atacctacaa caagtcggtt caaacgttta ttgacctgta
    34801 taccatcaaa cgtcgtacgc aggtgagtaa tatgctggca ttgagtcagg tttatttccc
    34861 catgtttgag gaggaactcg atcgcagagg aatgcctttg gagttaaaat atttgccggt
    34921 aattgaatcg gcgttgaata cgcatgcagt ttcgaaggcg ggtgcaacag gattatggca
    34981 attcatgctg ggaacagcta aattgaaagg attgcgcatt gatacctatg ttgatgagcg
    35041 tcgcgacccg catatggcaa cgattgcggg ttgtcaatat ctgcaagacc tctataatat
    35101 ttatggcgat tggttgcttg caattgccgc ctacaactgc ggtcccggaa atgtaaacaa
    35161 ggcgttgcgc aaagccggcg gtggtgataa aactttttgg gatatcgaac agtatctccc
    35221 caaagaaaca aaagcctacg tgccgatttt cattgcagca acgtacacat tcgaatatca
    35281 caaagagcac aatattcgcc ccgcaacttt tggttacgat tttagcatga gcgatacttt
    35341 gatgatcaca cataaaatga ccatcgatca gctagctcct tatgttggaa taagtgcaga
    35401 tgaaattgca ttgaataatc ccgcactgaa aacaaaaacc atcccgggat ctcctgttcc
    35461 atatccattg atactgccgc aaaaagcagt ggccgcgtat tatgccaata aggattcttt
    35521 gtatgcatcg cttgaacgca aagaacaaat tgcatcacaa caattggcta aaaatgtggc
    35581 tgcagtaaat gctgcaaatg ataaattgaa acccaaaccc gcaaccgttg ccggcacagg
    35641 cgacagcagt gtaacaaccg caacggcaac accgataaac actaccactc cagaccttac
    35701 ccttgcagat ccggacgcac cggttactgt gaattataca gtgaaaaaag gcgacaactt
    35761 gggttatata tcagattggt ttgattgcac ggttgctgat cttaagaaat ggaataaatt
    35821 aagttctacg cagattgttc ccggccagaa attgaaaatt accgttcctg ctaagtatga
    35881 acagcaatat gctcagataa acaacatgac gctgtcgcag aaacagaaac tcactgatat
    35941 tgcattgatc agccctgcat caacaactga taaagggccg gttgttacaa acgacatcat
    36001 cttctatacc gtaaaaccgg gcgatacgct ttggagtatc tcacaggctt
//
LOCUS       DAOQHY010000010        36020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_471_length_36020_cov_5.292229, whole genome shotgun sequence.
ACCESSION   DAOQHY010000010 DAOQHY010000000
VERSION     DAOQHY010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 36020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36020
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_471_length_36020_cov_5.292229"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1571)
                     /locus_tag="PLL28_01690"
     CDS             complement(<1..1571)
                     /locus_tag="PLL28_01690"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNF68053.1"
                     /translation="MTRIFTFLACICLCLLSTNLQAQTFSHVENTWVPDDGSSITFAI
                     DVSGLPDVIDTVFGLETVCINMDHTWDSDLDIRLVAPDGTSFLLVSGIGGDGDNFNNT
                     CFNESAATPVSFGSAPFSGSFIPMGDMGIANNGQNPNGVWQLLVYDTYAFADQGYMYD
                     WSITFGDDPATSSTFDSSILPIVKINTGGQIIQNEPKVTAAFQIIDHGAGMLNHPTDT
                     DYAFDGKILVELQGFTGPYYPKKNYDFDIVDDAGLEYDTVLLGLPSENDFILKAEYLD
                     LSLMKNQIAYTMSRNMGRYAPRTKYCEVLVDGEYMGVFSLTEKIKRDDNRVDIAKLDS
                     IDISGEELTGGYIIEINENFTPNDWESDYLPINYATCGLPVAYKMVEPRIENIQPEQL
                     DYIVAYVDSFEDALHGPDFLDTAVGYRNYISVKSFIDFMLVNEFSSNYDSYGRSTFLY
                     KEKNGQLFIGPPWDYDRGYLPWTTEGWVWEITHPAWPFPFWWDKFRDDPEFRNEVYCR
                     WTALRADVLSDEAFDT"
     gene            complement(1664..3103)
                     /locus_tag="PLL28_01695"
     CDS             complement(1664..3103)
                     /locus_tag="PLL28_01695"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HNF68054.1"
                     /translation="MMQVIQAHIKTIIAIAAIGVFLFFITIGSCDKENLLAAASLTSE
                     PDEALSGGSATVFDQTVNAFALPVPGLSPEDELLFFVGNSFFNQNWVQAPSSTTARDG
                     LGPYFNAKSCSGCHFKDGRGEPPATIGQLSEGLIVRLSIPGNGAHGEPDPDPMYGGQF
                     QDHAIDGVAYEGQYEIVYVEVPGYYPDGTSYSLRKPQINFLDLQYGAMSSDIMYSARV
                     APQMCGLGLLEAISEKDILAHADPDDRNGDGISGRANYVWNAIENKEQIGRFGWKANQ
                     PTILQQVSGAFNGDIGITSPYFKDQHITDAQSAQRKLVNGGDPEIDNDDLEKVHLYSA
                     TLAVPARRNVDDQDVLSGKQLFIKLGCNACHLDKVVTGNHPKFSVLSNQTIHPYTDLL
                     LHDMGDGLADGRPDFQASGSEWRTPPLWGIGLFKTVNKHTYYLHDGRARSIEEAILWH
                     GGEASTAKASFMQLSKAERDQLLLFLESL"
     gene            complement(3100..4263)
                     /locus_tag="PLL28_01700"
     CDS             complement(3100..4263)
                     /locus_tag="PLL28_01700"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HNF68055.1"
                     /translation="MMTVRGDFNRFYAGFFIAITCLSSSCKNDASTSSDLETKVVADY
                     ADHVFNTYTETLSAAKYLQSAVDGFLAAPSDSTLQEARNGWRIARAYYGKTEAFRFYD
                     GPIDNGEHGVEGLLNAWPLDEAYVDYVEGNSSTGIINDIKTYPEITRDLLVSLNEKGG
                     EENISCGYHAIEFMLWGQDLDTIAAGKRPYTDFIAGSLINDRRRTYLKLVTDLLMEHL
                     QYVTDQWSPKIQDNYRTAFLQADPKESLQKIFTGIGVLAKVELAGERMFTAYDNASQE
                     DEQSCFSDNTYADLQSNLDGIARIFFGNEGRTGLRQLLESKDPTAAQNVETQLKATQQ
                     AFEHMHQPFDRAIAVDAYRAETMQAILAVQKLGNDIANAASALQLTINVTNVQ"
     gene            complement(4344..4916)
                     /locus_tag="PLL28_01705"
     CDS             complement(4344..4916)
                     /locus_tag="PLL28_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68056.1"
                     /translation="MNFCSFKSLLRFGTTLLLFLPILLCAQDTIGYSEFRFTVIDVQG
                     NELASDKITAQIGDIFLRDTTQVDNYAAARVEYSLRDSCWILSQYAPRGYNYEIEIFR
                     NSDTVNLRLLELRKMTLIYPGYNEIDQTGCQFCLCYDIPIQKGVYMLDVPQRRESWKY
                     LRQVYINVRNLPTEFRDISAMQNWMFRSGK"
     gene            complement(4897..5877)
                     /locus_tag="PLL28_01710"
     CDS             complement(4897..5877)
                     /locus_tag="PLL28_01710"
                     /inference="COORDINATES: protein motif:HMM:NF025608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HNF68057.1"
                     /translation="MSKLSGYLRAIYHHEEYHGWFKRRRFFEGWYFKIVDKTQQHAFA
                     FIPGIAMDEQGNRQAFIQVLDGIHVTADYYKFDREFFHPNDRRFKVKIKENSFSMNRI
                     KLQLPEIRGQVHFSEHVKLPKTLLWPGIMGWYSYVPLMECYHQVISMDTELTGKLVVR
                     GQEIDFTGGRGYIEKDWGRSFPSSWIWMQSNHFANPKTSFKLSVAKIPWLNSSFVGFI
                     SVFLLNGKILRFATYTGAKLKEVAINENGTEVCLEDKRYILRVNAVKAAGAELKSPIQ
                     GLMEGKVKESMQAVMHITLFDKHENRLIFNDTGNNTGLEIAGDTHELLQF"
     gene            complement(6077..>7477)
                     /locus_tag="PLL28_01715"
     CDS             complement(6077..>7477)
                     /locus_tag="PLL28_01715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68058.1"
                     /translation="DVMDFTPYVVPKATNITGLDFPDGTVMYLSWTGDDVGGAGSRDE
                     FALDDVSITAIEGAPVSLATFAPAADVVDETAGTASVNISLSDNADCTVHVSMNAAST
                     ADGADATFAAFDHTFDIAGDPLYTFNIPLTDDLLDEPDETLILDLTYIAGACAVGLPS
                     TFTLLIMDDDVTPPPTYTVYDIAEIHGEDANGVATSIDAHAEITGVVHGINTWNGGLQ
                     FTLIDATDGISVFSYDNDFGYTVTEGDEITIQGVVTQFNGLTEIEPDTLWFIDGGNAL
                     ETSSIVTTLSEATESHMTTITNLSYVDVAEWLGDGSSFNVNLTNGVNTYTIRIDDNNE
                     LSTMTAPSLGTPYTLSITGIGGQYDTGSPYLDGYQLNPRYASDIVVVPTVGIYAPEQY
                     AIAVYPNPVIDACTITANELINSIRVINALGQVVYSASVNANHVSLQTGDWNAGLYQI
                     QLDINGNLSNTQVVVE"
     assembly_gap    7478..7577
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7635..9608)
                     /locus_tag="PLL28_01720"
     CDS             complement(7635..9608)
                     /locus_tag="PLL28_01720"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HNF68059.1"
                     /translation="MKKLLLSFALLSPLSIFAQLSIDALGTDVPVDLSDYAGAGFQPG
                     GGAGTLNSDIWSAEGFTDGAVDFGETNLTGDFARGIGGAGITTGGIYGLPMPDGNRIW
                     IQPTNDDFTPGSMTLRLQNNTGITLGGLNIAYSAWASNDQERANSLLCSYSYDGVTFT
                     PIPELDFISPEVSDLVLHQSDFSFLLTGITVDPAAMFYIRFTGDDVSGAGARDEFGIS
                     NISFTAFEPDAVPEYNFEPSDITIDESTPDGSFIFSISESADCSFSFGYDPAGTATPG
                     FDFGLMTFAVDFTAGGPTSQEIIFAIVDDFETEIDETAIVYVTSVTGACIAGGAPSST
                     ITIVDNDAVVPPIATFTTVGVDDDEAVGTITGTLEISETADCTIQLYLDGASTMENGL
                     DYTFLLPTFLTFTEGGPTSIDFEIPIIDDLEVEPTEMLLINMLPWTGTCVMGAIGDFE
                     INITDNDVEAPVYASFTFTGSEHNEGDGSTDIYVYLSEAADCTIEVTANASSTAVNGL
                     DYSTTLPAEFTFTAGGATSQTLTIDIINDLEIEGDETLNLDMAVTSGACAIGLYPNYA
                     LDIIDDDFESIQQTSGIITQMYPNPAFELLNIQTDISMDNWFIYDINGKLIRNGSIQS
                     REIQIDIHDLVAGTYQLRVSNANESAITNFVKQ"
     gene            9780..10346
                     /locus_tag="PLL28_01725"
     CDS             9780..10346
                     /locus_tag="PLL28_01725"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNF68060.1"
                     /translation="MINYPHIPSAGHLLISEPFMADPNFKRTVVLLCEHMQDTGTVGF
                     ILNKSLNVKVCDALMDFDIVQSELFYGGPVAQDTLHYLHRFGDKIEESFHVIDDIYWG
                     GNFEQIGDMLREGTLNPDHIKFFLGYSGWSPGQLEEEMDEKSWLLSKAKGHYVFDIGR
                     SELWKTVLNDLGGDYKMITNYPEDPRLN"
     gene            complement(10351..10995)
                     /gene="pdeM"
                     /locus_tag="PLL28_01730"
     CDS             complement(10351..10995)
                     /gene="pdeM"
                     /locus_tag="PLL28_01730"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HNF68061.1"
                     /translation="MPIAVSIKGNSFRLSPDFALYWQDLQWLIIADAHFSKETHFRKH
                     GIAIPAAILQEDLRRVDVLIRTYSPQRVVFLGDMFHSENNPGIDAFAKWRNGNNMPID
                     LVIGNHDILDRSRYADLNIDIHEDKIIANNIVLAHDDLSDIPEGHYLLHGHIHPCIRL
                     AGKAKQSMRLPCFWFGKEKGVLPSFGTFTGGHAITPNKTDQVFAIADAQIVRIN"
     gene            complement(11054..13504)
                     /locus_tag="PLL28_01735"
     CDS             complement(11054..13504)
                     /locus_tag="PLL28_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HNF68062.1"
                     /translation="MYDYDQIIASWYATRNWKQYDFQQELAAAYQQGYNGILNAPTGS
                     GKTFAMWLPIIADHMAKGNHQATSGLKVIWITPLRALAKDIRNALQEACDLFDMGWRV
                     EIRTGDISAKDKQAQKKQMPDVLIITPESLHVLLCQIGYETIFENLSCVVVDEWHELL
                     GSKRGVQVELAISRLRGIREDQKKQLRLWGISATIGNLVEAMEVLLGVDAPEHKSIII
                     RSEQKKQIDIHTVLPEHIDVLPWAGHIGLKLLPNIIDIIHRSTTTLVFTNTRAMAEIW
                     YHALLENDERLAGIMAMHHGSLSGEVRDWVEENLHNGALKAVICTSSLDLGVDFRPVD
                     TVIQIGSPKGVARFTQRAGRSGHQPGAVSTIYFIPTHALELVEISAIRAAMEENVIEK
                     RDPMVMSFDVLIQYMVTLSISGGFKSNELYREVKSTYAYHLMTPEEWQWLLQHITQGG
                     QTLQAYDEFNKVGRVGDVYRIMHKKAATRHRLSIGTIVSDTVVAIKLMNGGKLGSIEE
                     YFIASLKPGDVFWFAGHCLEFLMLREMTAFVKITKEQKAITASYMGGRMPLSSYMADM
                     MRKQLGQAAESNLHNFEYTHLQPLLDLQRDRSAIPAYDTILVETAESKEGLHIFIYPF
                     EGRVVHEVLAGLIAFRISKKQELSISVAMNDYGFELLCNHPVEINESVIRELFEIKGL
                     DDDVFQSVNATEMARRKFRDISVISGMVFQGYPGEQKRARHLQSSAQLIFDVLRKYEP
                     SNLLIKQAYDEILNDQMENARLRTALLRISKSKILIKHTDRFSPFAFPIVVDDLSRNK
                     LTSESLASRIQQMLRENK"
     gene            complement(13540..15987)
                     /locus_tag="PLL28_01740"
     CDS             complement(13540..15987)
                     /locus_tag="PLL28_01740"
                     /inference="COORDINATES: protein motif:HMM:NF023478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HNF68063.1"
                     /translation="MQNRMVIFAVMKKYICIHGHFYQPPRENAWLEIIEQQESAAPYH
                     DWNERINAECYGPNAASRILNDEQRIIDIVNNYSRISFNIGPTLISWIAQHDSDTYNA
                     ILQADKESLTHFDGHGNAIAQVYNHIIMPLASERDKETQVLWGIADFRHRFGRHPEGM
                     WLAETAVDTATLEVLAHHNIAYTILAPRQAKAIRNMQDDSWMPVDENTIDTTRPYLVK
                     LPSGKSITVFYYNGRISRDVAFAGLLNSGKKFAEALSSNEIALDHPSLINIATDGESY
                     GHHHYRGDMALASCLDYLKKDEDVVITNYGQFLAKFPPVYETKIHENSSWSCVHGVER
                     WRSNCGCTDGGNPGFHQLWREPLRNALNWLKDQADVIFEQELKTYTDDPWHLRNGYIT
                     VVLNRNEEEIDAFLLKGCGRKLQYEEKIRVIRLLEMQRHAMLMFTSCGWFFDEVSRIE
                     TKQIMQYADRVIQIAEHETSSTLKDQFILLLDKAPSNIEKYKTAAGVYVNEIHKQRLT
                     LTRVGIHHAVMSIFDNTMQQGFRLNYHVKPVFFEKLLSGDLVLVSGHLQLASTFTYAS
                     EQLFFSALYLGQHHVIGGYADKMDEDHFEEIFLNLRDAFRNSDISAITTTIHRVFPGT
                     FSINDVFHDEREFLIARILSADVHAAEDSYRAVYERTYTLMDMLRQQKKSIPPLLHAN
                     LIAVINADLKNWFTHPSGDLHKLDQLTEAAIKWEVTLDVEHIGHVAARRLYDMVLHLA
                     HAPHDMSLLENMSKIIIRLHELKVKYRLWKIQNKYFRIGRDFINNKAFMRTMGDEEYK
                     RWLLKFKAVGEQLKIRF"
     gene            16172..18262
                     /locus_tag="PLL28_01745"
     CDS             16172..18262
                     /locus_tag="PLL28_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68064.1"
                     /translation="MRKVILPTLLLIATVSSFGQASFDHTVIEVGNLGMYITNAGTLG
                     RPDVRNTPTGDPSMEYPINSGIEHLFEGGFWIGAIVDGQYLVSTSAKDAASGYTTGLA
                     GYEFTSEIGNTIAQRSSLTSSDYFNFDAISHQDLLVDISDKNVIVPGTAITIADHTQP
                     LYADIHLETYAWNYSFADYFVLLNYTITNNSTSVWDSVWCGIWNDMVVRNVNVSTDYG
                     SAFFSHGGYGYLGDDFTNYAFDVDGDPGFTNAYGAFQFLGIEWRDQFIHPNNNDLLTA
                     NGYPAAEVNSNFWIFNSTATPPYNAPANDVERYDKLKQGLNYADPDIVEFLKDPSTTG
                     GLTNLLSAGPMISVEPGETFHVVFAAVCAKQIENGGTTGVDMDTPEAQAELLEHLSWS
                     KRTYLGEDQNENGLLDIGEDLDADTILDRYILPEPPATPKMKVIADDQRIDIYWDNRA
                     EFSVDPISKEMDFEGYRLYRTQPGDDFNLNLIGDANMIAQWDLPANGIGFNNGFSAIA
                     LTTPEIIDEDTFYYKYTLDPVLNGWQYLIILTAFDRGDEDLHIESLESSFIENAVHVF
                     PGTSPNNDANAEIGVYPNPYRLQAAWDGSTGTTGKIMFYNLPEHCTISIMTLSGDIVA
                     ELNHDAATYTGSDSEWFATYAGNEDERIFSGGEHAWNLLSESNQTITQGIYLFSVKDL
                     DNGNIKQGRFAIIK"
     gene            complement(18281..20899)
                     /locus_tag="PLL28_01750"
     CDS             complement(18281..20899)
                     /locus_tag="PLL28_01750"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HNF68065.1"
                     /translation="MAIGAHSGNFVYNNVQTNEAFVYSSAQSVPDSSIAIDSTKNDSL
                     LLNGNDLWYAVSPDALDQIINYKGVDSIIYDLDSGKTYIYAKGEITYQSFNLKADFIV
                     FDWAEKTICAKQITDSTGKKGDLVYFKDGDEEFKAEYMCYNFGTKKGKIHYFRAQEGE
                     GYIAVEQAKKNDDDSYYGDHLTYTTCELDHPHFYITAEKAKVVPQKVAVTGPANLVIA
                     DVPTPLFLPFGIFPIKRGQTSGIIIPQYGNLTTQGFFLKGGGYYFALSDYYDLMLTGD
                     IYSRGSWGLHTSSRYNLRYKFNGALYLDYAVNKYDFPFSPNYSENTGYYIRWSHSQDA
                     KSTPNSKFSASVNLGSTNYLKDNSYSSSYLSNSLNSSISYGHNFVGTPFNFTAALRHS
                     QNISEKTVNATLPEAALTMNRIYPLKKLTDNKNSFMYQLGVTYSMNMQNSVSRPDSLF
                     FDQATLDAMRSGFQHKVSASAPVKVLKYFTFSPNVNYTENWYFESIRKRYDPYTVTDT
                     IQTGTGEDSIITTEYLVQVDTVEGFSAARYYSMGASLNTKIYSLAQFNGKLKAIRHVM
                     TPTLSFNYTPDFSLPKYGYYGEYYPTASQEEPTLYSIYEGGVYSGPGRGEMGTISLGI
                     GNTLEMKVYSKKDSVTHEKKVKLLENFSVSGGYNLAADSLNFSDIGFSAYTTLFKNIR
                     INVNGSFDPYILDTLGRNIDQFEWDVNHRIGRFNSGNISLSTDFQSKRNNNPSLATNA
                     GTEEEREMVWNDPSAYIDFEVPWSFAVGYNLRVTNAPTIDGRDSIYTTQSATFNGDLS
                     LTPNWKVQVTTGYDVQQKEFTYTSIDIYRDLHCWQMSFKWIPFGLRQSYVFNINVKAS
                     VLQDLKLTRKKDWSEY"
     gene            21018..22190
                     /locus_tag="PLL28_01755"
     CDS             21018..22190
                     /locus_tag="PLL28_01755"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNF68066.1"
                     /translation="MKKQISRTSVITGAFMVLWLGVCAFNTPSTPELPAYKLKTVVID
                     AGHGGHDIGCSGSANNEKTVALKLALKLGEMIEKNYPDVKVIYTRKTDVFVELYERAA
                     IANRNNADLFICIHCNANPNSSPYGTETYVMGLHKTEANLNVAKRENDVILMEDNYNQ
                     HYDGFNPNDPASHIIFSLNQHAFMDQSILFASSVEKYLKSHAKRSSRGVKQAGFLVLW
                     KTAMPSVLIETGFLTNSSEEKFLASESGQTTIAESIFYAFRDYKNDMENNSGKVSEAT
                     VQAIEANMDKEVETPKTEPVTTSTDNKGLTFSVQFYTAESEASATKFADLKPENVTYV
                     KENGLYKYRLGPYKTLDEATKKQTFARNAGYKDAFVIAVKDGKRISIEEAKALAGS"
     gene            22242..23180
                     /locus_tag="PLL28_01760"
     CDS             22242..23180
                     /locus_tag="PLL28_01760"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNF68067.1"
                     /translation="MKISNESKVGILAAVGIALLFIGFNYLKGINVFSKGIDYNVQYE
                     TSGGLLVGDPVIIDGLAVGRVKSVTLQQDQSGVDVILNFEQRLKIPADSYALIRGNLM
                     GEKYVQMFLGDSSELIPDGGTLTGKIESDLANTISQEFKPITDKVKTMLSSMDTAINV
                     LRGIFTEEVQNDFAKSMASIKTTLESFNNSADRVNALINKEESNIDSIITDVSGFTQT
                     VNQSKDEMTQILDNLKLISDSLKTVEWQSLAGEFQKAADNINAISAKINSGDGSLGML
                     VNDQQLYTDLTKTLTTLDSVLNEFGKDPTLRIKFFK"
     gene            23177..24718
                     /locus_tag="PLL28_01765"
     CDS             23177..24718
                     /locus_tag="PLL28_01765"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HNF68068.1"
                     /translation="MIPASDMRKSVRYIFQFILVATICCTDTIFVYGQSDSSAIQIKS
                     ADYLEIIQRKNVSINRLVHNVVLTQRNLTLYCDSAFLYKSENIARIYGHVHLSQSDSI
                     DAYGDSAIYDGNTRMAHLFGNVHLTDNSMTLTSKDLSYDMSSRVATYVNGGKVTDGEA
                     VLTSRTGYYYAFTNDAFFRNDVVLKHPKYQLEADTLQYNTGTETAFFHGPTRITNENS
                     TVLCRKGYYQTNTGIAVFHDQVTLNNPPQKLLADSIYYNRETGIGKAFRHIVFTDTAQ
                     QVIQFSNVAEYDEKNNSIFSTDGSVAGYVMEDDTLFIGGDTIRTAQDSLGRKTMYVFR
                     NVKIYKSDLQGLCDSLSYSDADSTLRMYGLPIMWSDSTQFTADTMHLVMLHQQLNQVQ
                     LFSNGFIINEDDSLIYNQIKGRNIYGYFSDNDLKKIKAIGNGESVYFGKDDANRYIGV
                     NTLYCSEINIYLSDKKFHRIAFKNAPDSKFQPMQNINLKDYQLKNFNWRYEERPSSLD
                     DLLKD"
     gene            complement(24726..25946)
                     /locus_tag="PLL28_01770"
     CDS             complement(24726..25946)
                     /locus_tag="PLL28_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNF68069.1"
                     /translation="MKMKIKAPLQPIRSAITLEGSKSISNRLLLMQALCTSGFEIRNL
                     SPSDDTTTLQRLLKQQPSISDVGAAGTTMRFLTAYYSTLEGERMLTGSARMQQRPIGV
                     LVEALEKLGADITYIEKKGYPPVRIKGKELPGGTIRIRADISSQYISALMMIGPTLKK
                     GLTLILDGKIASAPYIQMTLSTMQELGIDCKWEGATITIKNGSYRSVAMQAESDWSAA
                     SYHYSICALSPGSHMRISGLFEKSLQGDSVLPDIYAKLGVTTFFEGKEMIISNTGNCT
                     SLLDQDYSDCPDIAQTVAVTCAALGIPARFTGVESLRIKETDRTAALQSELAKFNVTF
                     EEREPGEWILSGKINPVEATISTYEDHRMAMSFAPLALVAPSITIEEPEVVKKSYPSF
                     WYDMARLGFVLENI"
     gene            complement(25946..27007)
                     /gene="aroB"
                     /locus_tag="PLL28_01775"
     CDS             complement(25946..27007)
                     /gene="aroB"
                     /locus_tag="PLL28_01775"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNF68070.1"
                     /translation="MVLQTASYPIYIDSSPEHHGALSAWIIAQQYEQLFVLCDGETEK
                     HCYPLLKPLLPEHQIFTIPSGEAYKNINTCITIWQSLAKCIAGRKSLLINLGGGVIGD
                     MGGFAASTYKRGIDFIQIPTTLLSQVDASVGGKLGIDLDGVKNLVGVFRDPKAVFISP
                     AYLTTLSERQVKSGFAEMLKHGFIADADYLRTLHNADLHDVSLVQQLIERSVRIKKDV
                     VEKDPYEKGLRKILNFGHTIGHAVETWSLLNDEDPLLHGEAIAIGMICEAQLSTVICG
                     FSESELMEVTNMLMHRYGKYPLEKVSVNALMDIMQLDKKNSQQKIRFSLLQHIGLCQY
                     DLVATDDQIAAALQFYCGL"
     gene            complement(27007..27294)
                     /locus_tag="PLL28_01780"
     CDS             complement(27007..27294)
                     /locus_tag="PLL28_01780"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNF68071.1"
                     /translation="MIIRIVKMTFDHRKVEDFLRIFSDAQHKIEAFPGCKGVDLTRDV
                     LQSNIFMTISIWENLASLDEYRKSELFKTTWSATKLLFSERPDAWSVEKIR"
     gene            complement(27291..28064)
                     /locus_tag="PLL28_01785"
     CDS             complement(27291..28064)
                     /locus_tag="PLL28_01785"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNF68072.1"
                     /translation="MPIVTLTSDLGVKDYYVAVLKGKLLSGAPQALIVDISHEIAPFN
                     IQEAAFVMRNACFHFPPSTIHLVSVHAESRSPVKAVAIRMRDQYFIGMDNGLFSLMVE
                     DAPDQIVEIPISHGQQSSFAAKDILVHAAISLLQGKTLQQIGTPLGSLETKTYLRPPD
                     NAYLIRANIAYIDRFGNLIINITKERFEKLRGGREFVINYKRTEEIREISSTYTDVPE
                     GEKLALFNSSGYLEIAIHKGNASQLLGLHLDNTIQIEFE"
     gene            28108..29109
                     /locus_tag="PLL28_01790"
     CDS             28108..29109
                     /locus_tag="PLL28_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNF68073.1"
                     /translation="MHERSKLPIKLIEVTIPLDGVRPIDFYGIHNARLGIIKKTFPFL
                     NISARGSELKVRGNEKDIEDFKHRIDLVINHLEKFNTISDSNLQSILFGVPETELVDL
                     PELPDDVIVHGPSGYLVRARTPNQKKMVSLATANDIVFAIGPAGTGKTYTAVALAVKA
                     LKEKVVKRIILTRPAVEAGENLGFLPGDLKEKIDPYLRPLYDALDDMLPIEKLNLYMQ
                     NRVIEIAPLAFMRGRTLDHSFIILDEAQNATDAQLKMFLTRIGPSAKCIITGDLTQID
                     LPRNQQSGLFTSLRILKDIDGIGTIFLNENDVVRHRLVKQIIKAYDVEKSRQEHHGE"
     gene            complement(29124..29768)
                     /locus_tag="PLL28_01795"
     CDS             complement(29124..29768)
                     /locus_tag="PLL28_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNF68074.1"
                     /translation="MENKTTYEQLLINNKDWVTEKLAEDPSYFGRLSVGQNPPFLYIG
                     CSDSRLPIDLFTGADPGKIFIHRNVANQVFLNDMNLLSVLEYAVKVLKVEHVIICGHY
                     KCGGVEAAYSGTTMNIVENWTMNIRDIAMEYKKELDAIEDYQERLNRLSELNVIRQLR
                     QLCKTSVMHAAFNEGAYPKLHGWVLDIAHGLINELPLPIDEWKEYGLLPAGYQS"
     gene            29931..30887
                     /locus_tag="PLL28_01800"
     CDS             29931..30887
                     /locus_tag="PLL28_01800"
                     /inference="COORDINATES: protein motif:HMM:NF017765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HNF68075.1"
                     /translation="MSGFLDPVVLFFILGALAGVLKSDLKVPEAFYHTLSIYLLLAIG
                     IKGGIELYHANPSGILLPAIATITAGITVTLIAALLLKRIAKFNNPDVWSIAGHYGSV
                     SAVTFAVILTFLNDKQVPYEEFMTVLLVTLEIPAIAFAIFAYKSSVSSGKIEIRKLAH
                     EVFFGKSILLLLGGLGIGYYTAAANNQQLNFFFFDLFKGFLAIFMLEMGIVASARFAD
                     LKKVGISLILFGVMMPLIGASIGILAGNIVGLSVGGTAVLATMCASASYIAAPAAMRI
                     AIPDANPTYSLTAALGITFPFNIIIGVNLYYRMSVIVDGILN"
     gene            complement(30904..31425)
                     /locus_tag="PLL28_01805"
     CDS             complement(30904..31425)
                     /locus_tag="PLL28_01805"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNF68076.1"
                     /translation="MANWKKPQPGEVFPYQEKYIALVEGDDVLEMLSQHVQQTIDMLA
                     GIDEQKALFRYAPGKWSVKEVLQHLIDTERIMCYRALRFARKDKTPLPGFEENDYAPV
                     SMADNRSMVSLLQEFAAVRHATIMLFESFTDDMIARTGTGNGKEISVNALGFIIVGHE
                     LHHMKLFKEKYLI"
     gene            31559..32044
                     /locus_tag="PLL28_01810"
     CDS             31559..32044
                     /locus_tag="PLL28_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68077.1"
                     /translation="MKKVFDSVKTALGETTGVLGEGYLAATDLLKETLSNFYDVKDLT
                     KEKMASITNDVIALAPIIEQTGYRAKEINIGVGIPPRMVFHFEKFADIKDEEIKRILA
                     ENKDKKLLSVLVNTLVSADAFQQKITLGSFSFNEISIELSVPPEVNVKFVNAATQQTV
                     I"
     gene            32047..33312
                     /locus_tag="PLL28_01815"
     CDS             32047..33312
                     /locus_tag="PLL28_01815"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNF68078.1"
                     /translation="MDIISLLTRLAFTAIFYFIPDIFIHGAVKASFTKNPKRALKRYW
                     SLNIALFFITSSGFLLMQFMSPDFARINLYFFGIFVSLLFAKLIVAIILLIAEDIWRI
                     PYSLFTRKKRNVESTPEKAPLLISRRKFIAKSALIAGALPFTGFMYGIFKGRYRFTIH
                     RETLYYSNLPDAFDGFTITQLSDLHMGSWDTNAKYHIQTLVDEANALKSDLILFTGDI
                     VNSRADEMSGWYEIVKQLQAPFGKISILGNHDYGDYVNWKTPEAKAANLALVKNIHPA
                     IGFNLLLNENTSIEKEGAKIYIAGVENWGKGDFPKFGDLDLAYKGIPNDAFMLLMSHD
                     PSHWDAKILEHPRLPNLTLSGHTHGFQMGIEVPAIRFSPSQFIYPQWAGLYTKKDTHI
                     YVNRGLGTVGYPGRLGIWPEITQITLRKK"
     gene            33329..33961
                     /locus_tag="PLL28_01820"
     CDS             33329..33961
                     /locus_tag="PLL28_01820"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68079.1"
                     /translation="MKYIIPVILLAVTLSCEKKEKQFDADGVLLAQFLESDSGSGLDF
                     SPMQNDDSSRVINFTPTIYNFKKKPYTGKVTAYDDKQRKIFDGNLNDGLPEGNWIFYY
                     ASGVVQIEGTYTKGLETGIWKNYYTVDKPKVIKQYDQSGYLLMRKEFYDTGKLKNYQN
                     VKCPQYGDIARRVQFKYNGAIEYLDAERELGKLDPAELQTVLLNDKLMVK"
     gene            34024..34677
                     /locus_tag="PLL28_01825"
     CDS             34024..34677
                     /locus_tag="PLL28_01825"
                     /inference="COORDINATES: protein motif:HMM:NF025029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HNF68080.1"
                     /translation="MRTLLLFVIACSVAAACESSDGMTTDETDEIVVKEVNIDSIDHC
                     YLVGKQAEAKAFCKSEGYNKHYCFLLDMSRHSGRYRFFIWDFDSNKVVDSALVSHGCG
                     ISNWGDDGTADAPVFSNEYESHCSSLGKYRVGERAWSQWGINIKYQLYGLDSTNSNAN
                     GRRIVLHSWEFVNDIEVYPDGSPEGWGCPAVSNAFMARLDTRLKKSTEPVLLWMYTH"
     gene            complement(34678..>36020)
                     /locus_tag="PLL28_01830"
     CDS             complement(34678..>36020)
                     /locus_tag="PLL28_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNF68081.1"
                     /translation="PPAGSCGGSYPGIGGNPLIYDNAENRIFMGGGGGQGSSNLGTGT
                     SGGNGGGIIIIIANNIEGNNFFIRSNGMSVTTTAFDDGAGGGGGAGAVLIDAASISSV
                     VNVQVHGGDGGDVDNTFDGVNCVGPGGGGSGGVLWMPSGAVPPNVNLDAGGGSSGITV
                     GEGAASPCFNSVNGATAGSNGNYVGGLALPIADQLYVELTLDMIPDDAVVCQGNELFM
                     SAVATGTGSVSYHWNDEDASTTPDVTVIPETDMTYTVIIEDDLGCQLIGEVVVDVIDT
                     VEITAFPDTSLEMGNYMTLYSNLDDTYTYLWSPDYNIDDVTAANPVINPYETTTYCVT
                     ATHPTGCSSTDCITILVAAGVAFPNAFTPNDDGINDVFRIPPVANLCEDIQYFKVFDR
                     WGQVVYDYYATTDSEGWDGTSGGIPQEIGTYVYLIKMLCDGENRTYSGALHLMR"
BASE COUNT        10679 a         7618 c         7245 g        10378 t
ORIGIN      
        1 gtatcaaatg cctcatcgct tagtacatcg gctctgaggg ctgtccagcg gcagtatact
       61 tcatttctga attccggatc atcgcgaaat ttatcccacc agaaagggaa aggccatgca
      121 ggatgtgtga tctcccatac ccagccttct gtagtccagg gtaaataacc gcggtcgtaa
      181 tcccaaggcg gacctatgaa taattggcca ttcttttctt tatacaaaaa tgtacttctg
      241 ccatagctgt cgtagtttga tgaaaattca ttcaccagca tgaaatcaat gaatgatttt
      301 acagatatat aatttctata acctacagca gtatccaaaa aatcggggcc atgaagtgca
      361 tcttcaaaac tgtcgacata tgcaacgata taatctagtt gctccggttg gatattttcg
      421 atgcgcggtt ctaccatttt ataagctacc ggtaatccgc atgtagcata atttattggc
      481 agataatcgc tttcccaatc attgggcgta aaattttcgt tgatttcaat gatgtatccg
      541 ccggtgagtt cttcacctga aatgtcaatt gaatctaact tggcaatatc aacccgatta
      601 tcgtcgcgct taattttttc tgtcaatgaa aatacaccca tatattctcc atctaccagc
      661 acttcacaat atttcgttct tggtgcatat cgtcccatat ttctcgacat ggtgtaagca
      721 atttgatttt tcatcagcga caggtcgagg tattctgctt tcagaatgaa atcgttttct
      781 gatgggagtc cgagcaatac ggtatcatat tcaagacctg catcatcaac aatatcgaaa
      841 tcataattct ttttaggata gtagggccct gtaaatcctt gcaactccac aagaattttt
      901 ccgtcgaatg cgtaatcggt atctgtaggg tgattaagca taccggcacc atgatcaatt
      961 atttgaaaag cggcagttac ttttggttca ttctgaataa tttgtccgcc ggtatttatt
     1021 ttcacaattg gcagaattga actgtcgaaa gtagaagacg tggcaggatc atctccgaag
     1081 gttattgacc aatcgtacat atatccctgg tcagcgaatg cataagtatc gtaaaccaac
     1141 aattgccaca caccattcgg attctgacca ttgtttgcaa ttcccatatc gcccattgga
     1201 ataaaagacc cactgaatgg cgcagatccg aaactcacag gcgttgctgc agattcatta
     1261 aaacaagtgt tgttgaaatt atcgccatct cctcctattc cgcttacaag caaaaaactg
     1321 gtcccatcag gcgccaccaa acgaatgtcg aggtcactat cccaggtatg atccatattg
     1381 atgcaaactg tttcaagtcc gaacacggta tcgatcacat cgggaagtcc ggaaacatca
     1441 atcgcaaaag tgatgctgga tccatcatcc ggcacccacg tattttcaac atgagaaaat
     1501 gtttgggctt gaaggttcgt ggaaagcagg catagacata tgcaggctaa gaaagtaaat
     1561 atgcgtgtca tacaaatagg caacacgaaa ttacatattt gccggcatta tggtaatact
     1621 ataccaaccc tttgcctgca attaaaaaag gatgactatt gttttacaat gattcgagga
     1681 agaggagcaa ctgatctcgt tcagctttgc ttaattgcat aaatgaagcc ttagctgttg
     1741 aagcttcacc tccatgccaa agaatggctt cttcgataga acgcgctctg ccgtcgtgga
     1801 gataataagt atgtttattt accgttttaa acaagccgat tccccacagt ggtggtgtgc
     1861 gccattcgct tcctgatgct tgaaagtcag ggcgaccatc ggcgagtcca tcacccatat
     1921 cgtgtaacag aaggtcggta taagggtgaa tggtttgatt cgataacaca gaaaatttcg
     1981 ggtgattgcc ggtaacaact ttatcgagat ggcaggcatt acaacctaat ttaatgaaca
     2041 attgtttccc actgagcaca tcttggtcat cgacatttct ccgtgcagga acagcgagtg
     2101 ttgctgaata gagatgtact ttttccaaat catcattatc aatctcagga tcgccaccat
     2161 tcaccaattt acgctgagca gattgcgcat cggtgatatg ttgatcttta aagtagggtg
     2221 aagtaattcc gatatcgcca ttaaatgcgc cgctcacctg ctgtaatatt gtcggttgat
     2281 ttgctttcca tccgaagcga ccaatttgct ctttattttc aattgcattc cacacataat
     2341 tagcgcgacc tgaaatgcca tcgccattcc tatcgtcagg atcggcatga gcaagaatat
     2401 ctttttccga aatcgcttcc aataaaccaa gaccacacat ttgcggagca acacgtgccg
     2461 aatacatgat gtcagatgac attgctccat attgcaaatc aagaaaattg atttgtggtt
     2521 ttctcaagga ataagaagtg ccatcgggat aatatccggg aacttctaca taaacaattt
     2581 catattgccc ttcgtaagca acaccatcaa tagcatggtc ttgaaattga ccgccataca
     2641 tcggatcagg atcaggttct ccatgtgcgc catttccggg aatacttaat cttacaatta
     2701 atccttctga taactgacca atggtagcag gaggctcgcc tcttccatct ttaaaatgac
     2761 atcccgaaca agattttgca ttaaaatacg gacccaatcc atcgcgcgct gttgtgctgc
     2821 ttggcgcctg cacccaattt tgattgaaga aagaatttcc gacaaaaaat aacaattcat
     2881 cttccggtga aagtcccggt acaggcagcg cgaaggcatt tactgtctga tcgaacactg
     2941 ttgccgatcc acccgataac gcttcatctg gctcagaagt taatgatgca gccgctaata
     3001 aattttcttt atcgcaggaa cctattgtga tgaaaaataa aaatacacca atggcagcta
     3061 ttgcaatgat ggttttaata tgtgcctgga tgacctgcat cattgcacgt tagtaacatt
     3121 gatggttaac tgaagtgcag atgcggcatt tgcaatatca tttccaagtt tttgcactgc
     3181 cagaattgcc tgcatcgttt cagcgcgata tgcatcaacc gcaatagctc tgtcgaatgg
     3241 ctgatgcatg tgttcaaatg cttgttgtgt tgccttgagt tgtgtttcca cattttgtgc
     3301 tgccgttgga tcttttgact ccaataattg acgcaatccg gtgcgacctt cattaccgaa
     3361 aaatatgcgt gctataccat cgaggttact ttgaagatct gcataagtat tatcactaaa
     3421 gcaactttgt tcgtcttcct gactggcatt atcatatgca gtaaacatgc gctcaccggc
     3481 aagttctact ttggcaagta cgccaattcc ggtaaatatt ttttgtaagg attctttggg
     3541 atctgcttgc aaaaatgcgg ttctataatt atcctgaatt ttcggcgacc actgatctgt
     3601 tacatattgc agatgttcca tcaacaaatc tgttacaagt tttaaatagg tgcgtcttcg
     3661 gtcgttgatc aggctacctg caataaaatc ggtatatggt cttttacctg ctgcgatagt
     3721 atctaaatct tgtccccaca acataaattc aatagcatga taaccgcaag aaatattctc
     3781 ttccccaccc ttttcattta atgacaccag caagtcgcgt gtgatctcag gataggtttt
     3841 gatgtcattg attattccgg tcgacgaatt tccttctaca taatcaacgt acgcttcatc
     3901 caacggccaa gcattgagga ggccttcaac accatgttct ccattgtcta taggtccgtc
     3961 gtaaaagcgg aaagcttcag ttttgccgta atatgctctt gctattctcc agccgttgcg
     4021 tgcctcctga agggttgaat ctgagggtgc tgctaaaaag ccgtcaaccg ccgactgcag
     4081 gtattttgca gcgctgagag tttctgtgta ggtattgaat acatggtcgg cataatctgc
     4141 caccactttg gtttcgaggt cggaagatgt agaagcatca tttttacaac tacttgataa
     4201 acaagttatt gcaatgaaaa aaccggcata aaagcggtta aaatcccccc gaactgtcat
     4261 cattaattta aaactagtct aaataaggtg caaatgtagg tagcaggagt gctttttttc
     4321 aatacccata agtaggtatt tgcttacttt ccgctgcgga acatccagtt ctgcatggca
     4381 ctgatatcgc ggaattcggt gggtaggttg cgtacgttga tatagacctg gcggaggtat
     4441 ttccaggatt cacggcgttg cgggacatcg agcatgtaga cacctttttg aattggaata
     4501 tcatagcaga ggcaaaattg gcatcctgtt tgatcgattt cattgtatcc ggggtagata
     4561 agagtcattt tgcgcaactc gaggagacgc agattgacgg tatcgctatt gcgaaaaatc
     4621 tcaatttcgt agttatagcc acgtggtgca tattggctca gaatccagca agaatctctt
     4681 aaagaatatt ctactctggc ggcagcataa ttatcgactt gagtggtatc gcgaagaaaa
     4741 atatcgccga tttgagcagt gatcttatca ctagccagtt catttccctg aacgtcgata
     4801 actgtaaaac ggaattcgga ataaccgatg gtatcctgtg cgcaaagcaa tattggcagg
     4861 aacaataaca aggtagttcc aaaccgcagc aaagacttaa aactgcagaa gttcatgtgt
     4921 gtcgccggca atttctaatc cggtattatt cccggtatca ttaaaaataa ggcgattttc
     4981 atgtttgtcg aaaagggtga tgtgcataac tgcctgcata ctctctttca ccttgccttc
     5041 catgagtccc tgaataggac ttttcaattc ggcacccgcg gctttaacag catttacgcg
     5101 aaggatatag cgtttgtctt cgaggcatac ttccgttccg ttttcgttga tggcaacttc
     5161 tttcaatttc gcaccggtat atgttgcgaa acgcaatatt tttccattga gcaggaatac
     5221 gctgataaat ccgacaaatg aactattcaa ccagggaatt ttcgcaacag aaagtttgaa
     5281 tgaagtttta ggatttgcaa agtgatttga ttgcatccag atccatgagg aagggaatga
     5341 gcgtccccaa tctttttcga tatatcctct tcctccggta aaatcaattt cttgtccgcg
     5401 caccaccaat ttcccggtaa gttctgtatc catgctaatc acctgatgat aacattccat
     5461 caaaggcaca taagaatacc atcccatgat accgggccac aataatgttt tcggaagttt
     5521 gacatgttcg ctgaaatgca cttgcccgcg tatttcaggc aattgcaatt tgatgcgatt
     5581 catagagaag ctgttctctt tgatttttac tttgaatcgg cggtcgttgg gatgaaaaaa
     5641 ttcgcggtcg aacttataat aatctgccgt aacatgaatg ccatccaaca cctgaataaa
     5701 agcctgtctg ttgccctgtt cgtccatggc aatgccagga ataaaagcaa atgcgtgttg
     5761 ttgagttttg tctacgattt taaaatacca tccctcaaaa aagcgacgtc gcttaaacca
     5821 tccatgatat tcttcatgat gataaatcgc acggaggtat ccggataatt tcgacattgg
     5881 cagaaatata tacatcaaac ttactcaatt aatacagtat tgctgttatg ataagttaag
     5941 aatgtattaa ggaacgccat atttacgata taatgacagt gctgacaagt agtttaggag
     6001 aagcttctgc acactgaaaa taaaaaagag ggatcagcag tctgacccct cttttgaaat
     6061 atgatttaac aataaattat tcaactacca cttgcgtatt gctcaagttt ccgttgatat
     6121 cgagttgaat ttgatacaag cctgcattcc aatcgccggt ttgcagtgaa acatgattgg
     6181 cattcactga tgctgagtaa acaacctgac caagcgcatt aatcacgcgg attgagttga
     6241 ttaactcatt agctgtaatg gtgcaggcat ctataacagg attcgggtaa acagcgatag
     6301 catattgttc aggagcataa atgcctaccg ttggcaccac aacaatatcc gaagcataac
     6361 gtggattgag ctgataacca tcgaggtaag gagagcctgt gtcgtattgg cctccaatgc
     6421 ctgtgataga aagtgtataa ggcgttccga gcgacggtgc tgtcatggtt gacaattcat
     6481 tattatcatc aatacgaatg gtataggtat ttacaccatt ggtaaggttt acattaaatg
     6541 atgaaccatc tcccaaccat tcagctacat caacataact aaggttagta attgttgtca
     6601 tatgcgattc ggttgcttca gagagcgtag taacaattga tgaagtctcc aatgcatttc
     6661 cgccatcaat aaaccacagt gtatcaggtt caatttctgt aagtccatta aattgagtga
     6721 caacaccttg aatagtaatt tcatcgcctt cagtcacggt ataaccgaaa tcattatcat
     6781 agctaaatac actaatgccg tctgttgcat ctattaatgt aaattgcaat ccgccattcc
     6841 aggtattaat gccgtgtact acacctgtaa tttcagcatg cgcatcaatt gaagttgcca
     6901 caccgttagc gtcttcgcca tggatttcag cgatatcgta tacggtatat gttggcggag
     6961 gtgtaacgtc atcatccata atcaacaaag taaatgttga tggaagtccg acagcgcaag
     7021 cacctgcaat atatgtgagg tcaaggatga gcgtttcatc aggttcatcc aataaatcgt
     7081 cggtcaatgg aatattgaat gtgtagagcg gatcaccggc aatatcaaaa gtatgatcga
     7141 aggcagcgaa tgtagcatct gcaccatcag ccgttgaagc tgcgttcatg ctgacatgca
     7201 cagtgcaatc tgcattatcg ctgagtgaaa tattcacgga tgcggtgcct gcagtttcgt
     7261 ctaccacatc agcagcggga gcaaaagttg ccaatgaaac aggcgcacct tcgatagcgg
     7321 taatggatac atcgtccaaa gcaaattcat cacgtgatcc ggcaccacca acatcatcgc
     7381 cggtccagct caaatacatt actgttccgt caggaaaatc gagaccggtg atattagttg
     7441 cttttggtac tacgtacggt gtaaaatcca tcacatcnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnagt aataaaccgg ctgatgccat aattgtcagt aaaattttct
     7621 tcatcagaaa taaattattg tttcacaaaa ttagtaatag cactttcatt tgcattcgaa
     7681 actctcaatt gataagttcc tgcaacaagg tcatgaatat cgatttggat ttcacgggat
     7741 tgaatacttc cgttgcgaat taattttcca ttgatatcat aaataaacca attatccatt
     7801 gaaatgtcag tttgaatgtt gagtaattca aacgccggat ttggatacat ttgagtaata
     7861 ataccggatg tttgttgaat agattcgaag tcatcgtcaa taatatccag tgcataattt
     7921 ggatataagc cgatagcaca tgctccggag gtgacagcca tatccagatt caaagtttca
     7981 tcgccttcaa tttcaagatc attgatgata tcgatggtta atgtttgtga tgtagcacct
     8041 cctgcggtga atgtaaattc agcaggtaat gttgtactgt agtccaaacc attaactgca
     8101 gtagagctag cattggcagt tacctctatt gtgcaatctg cggcttcaga taaatagaca
     8161 taaatatctg ttgaaccgtc gccttcgttg tgttcagatc cggtaaacgt aaatgatgca
     8221 taaacaggag cttccacatc attatcagtg atatttattt cgaagtcgcc gatagcgccc
     8281 attacgcatg tgcctgtcca tggcagcata ttgataagca gcatttctgt cggttctact
     8341 tccaaatcat caatgatagg aatttcaaaa tcaatagaag ttggtccgcc ttcagtaaat
     8401 gtgaggaagg ttggcaataa gaatgtatag tctaaaccat tttccatggt agatgcgcca
     8461 tcaagataaa gctgaatagt acaatcagca gtttcagata tttctaatgt tcctgtaatt
     8521 gttccaacag cttcgtcatc gtcgacacca acagtggtga aggtggcgat tggaggcaca
     8581 actgcatcat tatcaacgat agtaattgtt gagcttggag cacctccggc aatacatgct
     8641 cctgttacgg aagtcacata gactattgca gtctcatcaa tttctgtttc gaaatcatct
     8701 acaatagcaa aaataatttc ctgcgatgtt ggtcctcctg cggtaaaatc aactgcaaat
     8761 gtcatcagtc caaaatcgaa acccggtgtt gctgttcctg cgggatcata accgaaagaa
     8821 aatgagcaat cggcactttc gctgatagaa aagatgaatg atccatcagg tgtagattca
     8881 tcgatagtaa tatctgaagg ttcgaaatta tattccggta ctgcatcggg ttcgaaggcg
     8941 gtaaatgata tattgctgat tccaaattca tcgcgcgcac cggctccgga aacatcatca
     9001 cctgtaaaac ggatataaaa cattgcagca gggtctacgg taatacctgt cagcaaaaag
     9061 ctaaaatctg attgatgtaa aaccaggtca gacacctcag gagaaataaa atccaattca
     9121 gggataggcg tgaaagtaac accatcatac gaatatgaac agagtaaact atttgctctt
     9181 tcctgatcat ttgatgccca ggcgctgtat gcaatattaa gtccacccaa ggtaatgccg
     9241 gtattattct gcaaacgcaa ggtcatagaa ccaggggtaa agtcgtcgtt agtaggctga
     9301 atccaaattc tgttgccatc gggcattggc agcccataga tacctcctgt tgtaattccg
     9361 gccccaccga tgccacgggc aaagtcgcct gtgaggttgg tttcgccaaa atctactgca
     9421 ccgtcggtaa agccttccgc actccagata tcagaattga gggttccggc acctccaccg
     9481 ggctggaatc cggctccggc gtagtcagac aggtcaacag gcacatctgt tccaagcgca
     9541 tcgatgctca gctgagcaaa aattgaaagt ggagacaaga gtgcaaatga gagtagcaat
     9601 tttttcatca gaagggtatt ttttaatggt ataggtgtaa aaataagcac tatttgcggg
     9661 atccttgggt ctgaacaggc atgaaatatg tatatatgca gcataaattt gatgttaagc
     9721 ggttggcata ttgccgatca ctttgcatat ttggggctta aaccgacagt gcagcaagca
     9781 tgatcaacta tccacatata ccttcggcag gacatttgct tatcagtgaa ccatttatgg
     9841 cagatccgaa tttcaagcga actgttgttt tgttatgtga acatatgcag gataccggaa
     9901 cggtaggctt tattctgaat aagtcgctga atgttaaagt ttgcgatgcc ctgatggatt
     9961 ttgatattgt gcagagcgaa ttattttatg gtggacctgt agctcaggat acgttgcact
    10021 atttacatcg atttggcgat aaaattgaag aaagctttca tgttattgat gacatttatt
    10081 ggggaggaaa ttttgaacag ataggtgata tgctgcgcga aggcacttta aaccctgacc
    10141 atatcaaatt ctttttggga tatagcggat ggagtcccgg acaattggag gaagaaatgg
    10201 atgaaaaatc atggctctta tcaaaagcaa aagggcatta tgttttcgat atcgggcgca
    10261 gtgaattatg gaaaacagta ttaaatgatc ttggcggcga ttataaaatg atcaccaact
    10321 accctgaaga tccgaggctg aattaagatt ttagtttatt ctgacgattt gcgcatcggc
    10381 aattgcgaat acctgatcag ttttgtttgg agtgatggca tgcccacctg taaatgtgcc
    10441 gaaagatggc aacacacctt tttctttgcc aaaccaaaag catggcaatc gcatggattg
    10501 tttagccttt cctgcgaggc gaatacaggg atgtatgtgt ccatgcagca aataatgacc
    10561 ttcaggtata tcagataaat cgtcgtgtgc aagtacaata ttattggcga ttattttatc
    10621 ttcatgaata tcaatattaa gatcagcata gcgtgagcgg tctaatatgt cgtgattgcc
    10681 gattacgaga tcgatcggca tattatttcc gtttcgccac ttcgcgaaag catctattcc
    10741 gggattattt tcactgtgaa acatatcacc aaggaatact actcgttgag gggaataagt
    10801 tctaatgagc acatctactc gcctgagatc ctcctgcaaa attgcagccg gaatggcgat
    10861 gccatgtttc ctgaaatgtg tttctttaga aaaatgtgca tcggcaatga taagccattg
    10921 caggtcctgc caatagaggg caaaatccgg cgacaatcga aaagaattgc cctttatgga
    10981 aacagcaatc ggcatgagac aaaggtaata cttactataa atttagttta caggagaaat
    11041 gcaacgccaa cacttattta ttctctctca acatttgttg aatccgcgat gccaggcttt
    11101 ctgatgtgag tttattgcgc gacaaatcat caacaacaat agggaatgca aaaggtgaaa
    11161 agcggtcggt atgtttaatc agaattttcg atttcgaaat gcgcaataag gcggtacgaa
    11221 gtcgcgcatt ttccatctga tcatttaata tttcatcata ggcttgttta ataagtaggt
    11281 ttgaaggttc atatttccgc agcacatcaa aaatcaattg tgcacttgat tgcagatgcc
    11341 tggctctttt ctgttcaccg ggataacctt gaaaaaccat tccggaaatg acagaaatat
    11401 cacgaaactt tcttcgcgcc atttcagttg catttacaga ttgaaataca tcatcgtcga
    11461 ggcctttaat ttcaaataat tcccgaatca cagattcatt gatttcaacc gggtgattgc
    11521 acaataattc aaaaccataa tcattcatgg ccactgaaat actgagttcc tgttttttac
    11581 ttatcctgaa tgctattaaa cccgcaagca cttcatgcac aacacgccct tcaaagggat
    11641 aaataaatat gtgaagtcct tctttgcttt cggcagtttc tacaagaata gtatcgtaag
    11701 ccgggatggc cgacctgtcg cgttgcaaat ccaataaagg ctgaagatgc gtatattcaa
    11761 aattatgcaa gtttgattcg gctgcctgtc caagttgttt gcgcatcata tctgccatat
    11821 aactcgacaa gggcattctt ccacccatgt atgatgccgt aatggctttt tgttccttgg
    11881 taattttcac aaatgccgtc atctccctga gcatcagaaa ctcaagacaa tgtccggcaa
    11941 accaaaatac atcgccgggt ttcagggatg caataaaata ttcttcaata ctacccaact
    12001 tgccaccatt catcagttta attgcaacta ccgtatcgct cacaatagtt ccgattgaaa
    12061 gccgatgccg cgtagcagct tttttgtgca taatgcgata tacatctcct accctgccga
    12121 ctttattaaa ttcatcatat gcttgtaatg tttgtccgcc ctgcgttata tgctgcaaca
    12181 accattgcca ttcttcggga gtcatcaaat gataggcata agtgcttttt acttcgcgat
    12241 ataattcgtt ggatttaaat ccgccgctga tactgagcgt gaccatatat tgaatcaata
    12301 catcaaaact catcaccata ggatcacgtt tctcaattac attttcctcc atagcagctc
    12361 gaattgcaga aatttctacc aattcaagtg catgtgtcgg aataaaataa atggttgaaa
    12421 cagctcccgg ttgatgtccg cttcttccgg cgcgttgtgt aaatcgtgca acaccttttg
    12481 gtgaaccaat ttgaattacg gtatcaacag gtcggaagtc aacaccgaga tctaaactgg
    12541 aagtacatat cactgccttt aatgcaccat tgtgcaaatt ttcttcaacc caatcccgca
    12601 cttcaccgga taaactgccg tgatgcatgg ccataatgcc agccaatcgc tcatcattct
    12661 ctaataatgc atgataccat atttcagcca tggcacgggt attggtgaat accaacgtag
    12721 tagttgaacg atgaataata tctatgatat taggtaataa ttttaatccg atatgtcccg
    12781 cccagggtaa aacatctata tgctcgggta ataccgtatg aatatcgatt tgcttttttt
    12841 gctcactgcg gataatgata ctcttgtgtt ccggagcatc tacgcctaac aggacttcca
    12901 ttgcttcaac cagattacca atggttgcag aaatccccca gaggcgcaat tgtttttttt
    12961 gatcttcacg aattccgcga agcctgctga tagctaattc aacctgtact ccacgtttgc
    13021 ttcccaataa ttcatgccat tcatccacca cgacacaaga aagattttca aaaattgttt
    13081 cgtaaccgat ctggcataac aaaacatgca aactttcggg agtgatgatg agcacatcag
    13141 gcatttgttt tttttgcgcc tgtttgtctt ttgcagagat gtcaccggtt ctgatttcca
    13201 cacgccaacc catgtcaaaa agatcgcagg cctcctgaag tgcattgcga atatcttttg
    13261 ccaatgcacg taagggagtt atccaaatta cttttaatcc acttgtggct tggtgatttc
    13321 cttttgccat atgatcggca ataatcggca gccacatggc aaaagtttta ccgctaccgg
    13381 taggcgcatt tagaattccg ttatatccct gctgataggc tgcggcgagt tcctgctgaa
    13441 aatcgtattg tttccagttt ctggtggcat accagcttgc tataatctga tcgtaatcgt
    13501 acacgatata ggcacacaaa aatgcagtcc aaaatacaat taaaacctga tcttcaattg
    13561 ttcgcccact gctttaaatt tcagcaacca gcgcttatac tcctcatctc ccatagtacg
    13621 catgaaggct ttattattga tgaaatcgcg tccaatgcgg aaatatttat tctgaatttt
    13681 ccaaaggcga tatttcactt ttaattcgtg cagtcggatg atgatcttac tcatattttc
    13741 gagtagcgac atatcatgtg gtgcatgagc aagatgcaat accatgtcat ataatcttcg
    13801 tgcagccaca tgtccgatat gttcaacatc cagtgtcacc tcccatttga tagccgcttc
    13861 tgtcaattgg tccagtttgt gcagatctcc tgaaggatgt gtaaaccaat ttttgagatc
    13921 ggcgtttatc acggcgatta aattggcgtg caacaatggc ggaatagatt ttttttgttg
    13981 acgaagcata tccatcaaag tatatgttcg ttcataaact gcgcgatagc tatcttctgc
    14041 ggcatgcaca tcagcactta aaattcgtgc gatcaaaaat tcgcgctcat cgtgaaatac
    14101 atcgttgata gaaaatgtgc ccggaaaaac gcgatgtatg gtggtggtaa ttgcgctgat
    14161 atcactattt ctgaaggcat cgcgcaggtt gaggaaaatt tcttcaaaat gatcctcatc
    14221 cattttatca gcatatccac caataacatg atgttgcccc agatataatg ctgaaaaaaa
    14281 caattgttcg ctggcataag tgaatgtgga cgccagctgc aagtggccgc ttaccaatac
    14341 gagatcaccg gacaataatt tttcaaaaaa tacaggtttt acatgataat tcaatcgaaa
    14401 tccctgctgc atagtattgt cgaaaatact catcacggcg tgatgaatac caacacgtgt
    14461 aagcgtcaaa cgttgtttgt ggatttcatt gacatataca ccagctgccg ttttatattt
    14521 ctcgatattt gagggtgcct tatctaataa gagaataaat tgatctttga gggttgatga
    14581 agtttcatgc tctgcaattt gtattaccct gtcggcatac tgcatgattt gctttgtttc
    14641 aattctgctt acttcatcaa aaaaccatcc acaactggta aacatcaaca tcgcatgacg
    14701 ttgcatttcg agcagtcgaa ttactcttat tttttcttca tactgcaatt ttcttccgca
    14761 gcccttcaat aaaaatgcat caatttcttc ttcgttgcga tttaatacta cggtaatata
    14821 accatttcgt aaatgccatg gatcgtccgt atatgttttc aattcctgtt caaaaatgac
    14881 atctgcctga tctttcaacc agttcaacgc attgcgcaaa ggttcgcgcc aaagttgatg
    14941 gaatccggga tttccgccat cggtacatcc gcaattactc ctccatcgtt caacaccatg
    15001 cacacaactc caggagctgt tttcatgaat ttttgtttca taaaccggag ggaatttcgc
    15061 gagaaactga ccataatttg taatgacaac gtcctcatcc tttttcagat aatcgagaca
    15121 tgaagcgagg gccatatctc cgcgataatg atgatgacca tatgattcac catctgtggc
    15181 aatattgatc aatgaaggat gatcaagggc aatttcatta ctactcagtg cttccgcaaa
    15241 tttctttccg gaattcaaca atccggcaaa ggcgacgtcg cgggatattc tgccattgta
    15301 atagaaaacg gtaattgatt ttccgctagg caatttcact aaatatgggc gagtggtatc
    15361 aatggtattt tcatcaaccg gcatccagct atcatcctgc atattgcgaa tggcttttgc
    15421 ctgacgaggg gcaagtatgg tgtatgcgat attgtgatgt gccaagacct cgagcgtagc
    15481 ggtatctaca gccgtttcag cgagccacat accttcaggg tgtcgtccaa aacgatgcct
    15541 gaaatctgca attccccaca gcacctgtgt ttccttatct cgctcagagg caaggggcat
    15601 gatgatgtga ttatagacct gggcgatggc gtttccgtgg ccgtcaaaat gcgtgaggct
    15661 ttccttatct gcctgcaaaa tggcattgta ggtatcactg tcgtgttgcg caatccacga
    15721 tatcagggta ggtccgatat taaaactgat acggctgtaa ttattgacga tatcgataat
    15781 tcgttgctca tcatttagaa tacgtgacgc cgcattaggt ccgtaacact ccgcatttat
    15841 gcgctcattc caatcatgat aaggggctgc cgattcctgc tgctcgataa tttccagcca
    15901 tgcattctca cggggcggct gataaaaatg cccgtgaatg cagatatact tcttcattac
    15961 tgcaaaaata accattcgat tctgcatttg agcatatatt aatcatcatt cgaatcggaa
    16021 acatttgcgc atgtttttca aaatttattg taatgttcac gggaatcctt acttcaaaat
    16081 tgaagcaact tccatataca atgcataggg aacagatttc atcaattgtt gaggtgtgag
    16141 gcaattaatt aaggttccgt aactttgacc catgcgcaag gtgattttac ctacactgct
    16201 attgattgca actgtcagtt cgttcggaca ggcctccttc gatcatacag taattgaagt
    16261 gggtaatctc ggaatgtata tcaccaatgc cggcacgctt ggtcgtcctg atgtgcgcaa
    16321 tacccctacc ggtgaccctt caatggaata tccgattaat tccggaattg aacacttatt
    16381 tgaaggcgga ttctggatag gtgctattgt cgacgggcaa tatttggtaa gcacctcagc
    16441 caaggatgct gcaagtggtt acactaccgg acttgcagga tatgaattca catctgaaat
    16501 cggaaatact attgctcaac ggtcatccct tacttcaagt gattatttta atttcgacgc
    16561 aatttcacat caggatttat tggtagatat ttccgataaa aatgtgatcg ttcccggcac
    16621 tgccatcact attgcggatc atactcaacc actgtatgcc gatattcatc tggaaactta
    16681 tgcatggaat tattccttcg cagattattt tgtgttactg aattatacca tcacaaataa
    16741 ttcaacttca gtttgggata gcgtatggtg cggcatctgg aatgatatgg tggtgcgcaa
    16801 tgtgaatgtt tccaccgatt atggctccgc atttttcagt catggcggat acgggtatct
    16861 tggcgacgat tttacaaatt atgcattcga tgttgatggc gatcccggtt tcacaaatgc
    16921 atatggtgct tttcaatttc taggtataga atggagagac caatttattc acccgaacaa
    16981 caatgacctg cttacagcaa atggttatcc cgctgctgaa gtaaatagta atttctggat
    17041 attcaattca acggcgactc caccatataa tgcaccggcc aatgatgtgg aacgctatga
    17101 taaattaaag caaggtctta attatgctga tcctgatata gttgaatttt taaaggaccc
    17161 cagtacaaca ggaggactta caaatttact ttccgcaggt ccgatgataa gtgttgaacc
    17221 gggagaaact tttcacgtag tatttgcagc agtttgcgcc aagcagatag aaaacggagg
    17281 cactacgggt gtggatatgg atacacctga agctcaagcc gaattattgg aacatctcag
    17341 ttggtcgaag cgcacctacc ttggcgaaga tcaaaatgaa aatggattat tggacattgg
    17401 tgaagacctt gatgccgata ccatactcga cagatatata ttacctgaac caccggcaac
    17461 gccaaaaatg aaagtcattg ccgatgatca gcgcatagat atttattggg acaatcgcgc
    17521 agaattttcg gtagacccta tttcaaaaga aatggatttt gaaggatatc gattgtatcg
    17581 cacacaaccc ggtgacgatt ttaatctcaa ccttattggt gatgcgaata tgattgctca
    17641 atgggatctt ccggctaacg gtatcggatt taataacggg ttttcagcta ttgcccttac
    17701 aactccggaa attattgatg aggatacatt ttattacaaa tacacacttg atcccgtatt
    17761 aaacggatgg caatatttga taattcttac tgcattcgac cgcggtgatg aagaccttca
    17821 tattgaaagc ctggaatctt cttttattga aaatgctgtc catgtgtttc cgggtacttc
    17881 accaaataat gatgcaaatg ccgaaattgg tgtttaccca aatccatatc gcctccaagc
    17941 cgcctgggac ggaagcacag gaacaacagg taagatcatg ttttacaatc ttcctgaaca
    18001 ttgtaccatt tctatcatga cattgagtgg cgatattgtt gcagaactca atcacgatgc
    18061 tgcaacctat accggtagcg acagtgaatg gtttgcaacc tatgcaggca acgaagatga
    18121 acgcatattt agtggcggag aacatgcctg gaacttgttg agcgaaagta accagacaat
    18181 tacacaagga atttatttat tcagcgtgaa agaccttgac aatgggaata tcaaacaagg
    18241 tcgatttgct attattaaat gatgatcaat taattgaatc tcaatattct gaccagtcct
    18301 ttttacgtgt aagttttaaa tcctgcagca cactggcttt tacattgata ttaaagacat
    18361 aagactgtct taatccgaat ggtatccatt tgaaactcat ctgccagcaa tgtaagtcac
    18421 ggtaaatatc aatactggtg taagtaaatt ccttttgttg aacgtcataa ccagtagtta
    18481 cctgcacttt ccaatttgga gtaaggctta aatcaccatt aaatgttgcc gactgagtag
    18541 tataaatact atcacgccca tctattgttg gagcattggt gacacgtaaa ttatagccga
    18601 cagcaaagct ccaaggcact tcgaaatcga tataagctga cggatcgttc caaaccattt
    18661 cacgttcctc ttcagtgccg gcgttggttg cgagtgaagg attattattc cgcttggact
    18721 gaaaatctgt actcaaacta atattaccgc tattgaatct accgatacga tgattaacat
    18781 cccattcaaa ctgatcgata ttccgaccta aggtatccag aatatatgga tcaaaacttc
    18841 cattcacatt gatgcgaata tttttgaata aggtagtgta ggcactaaat ccgatgtcag
    18901 aaaaattgag tgaatcagca gccagattgt atccgccgct aacgctgaag ttttccagaa
    18961 gttttacttt cttttcatgg gtaacactat ctttctttga atacactttc atttccagtg
    19021 tgtttccaat tccaagtgaa atagttccca tttcacctct tccgggaccg ctgtaaacac
    19081 caccttcata aatggaatac aatgtaggtt cttcttgtga agcggttgga taatattcgc
    19141 cataatagcc atatttcggc aaactaaaat cgggggtgta attaaatgac aatgtaggtg
    19201 tcatcacatg cctgattgcc ttcaacttac cattgaactg tgctaaggaa taaattttgg
    19261 tgttcagcga ggcacccatg gaataatatc gtgcagcact aaaaccttca acggtatcta
    19321 cctgcactaa atactcggtg gtaatgatac tatcttctcc tgtcccggtt tgaattgtat
    19381 cagttaccgt ataaggatca taacgtttgc gaatggattc gaaataccag ttttcggtat
    19441 aatttacatt agggctgaaa gtgaaatatt ttaaaacttt caccggtgca gatgcagata
    19501 ccttatgttg aaatccgctt cgcatggcat caagtgtcgc ctgatcgaaa aataatgaat
    19561 ccggcctgga aacagaattt tgcatattca tagaataggt aactcccagc tgatacataa
    19621 agctgttttt attatctgtc agctttttca gagggtaaat acgattcata gtcaaagctg
    19681 cttcaggtaa ggtggcgtta actgtcttct cgctgatatt ctgactgtgg cgcaatgcag
    19741 cggtaaagtt gaaaggtgtt ccaacaaaat tatgcccata ggaaatgctg gaattgagtg
    19801 aattcgaaag atagctgctt gaataagagt tatctttcag ataattggta gaccccagat
    19861 tcacacttgc actgaatttt gaatttggag ttgatttagc atcctgacta tgcgaccatc
    19921 tgatataata tcctgtattt tctgaataat tagggctgaa gggaaagtca tatttattaa
    19981 cggcataatc gaggtaaagt gcaccattaa atttatacct caaattatat ctcgagctgg
    20041 tatgcaatcc ccatgagcca cgtgaataaa tatctcccgt taacatgaga tcataataat
    20101 cgctcaaggc aaaataatac ccgccacctt tcaggaaaaa accctgtgta gtcaaattac
    20161 catattgcgg tatgatgatg cctgaagttt gtccgcgctt aataggaaaa attccgaatg
    20221 gcagaaacaa gggcgttgga acatcagcaa taacaaggtt agccggaccg gttacggcaa
    20281 ctttttgagg tactactttt gctttttccg ctgtaatata aaaatgcgga tgatctaatt
    20341 cacaagtagt atatgttaag tgatcaccat agtatgaatc gtcatcgttt ttctttgcct
    20401 gttcaactgc aatatatcct tcaccttcct gcgctcggaa ataatgtatt tttccttttt
    20461 tcgtgccgaa attgtaacac atgtattccg ctttgaactc ctcatctcca tctttaaaat
    20521 aaaccagatc gccctttttt ccggtgctat cggtaatttg ttttgcgcaa atggtctttt
    20581 ccgcccaatc gaaaacaatg aaatcggcct tcagattaaa cgattgataa gtgatttctc
    20641 ctttagcata gatgtatgtt ttgcctgagt caaggtcata aataatagag tctacgccct
    20701 tataattaat gatctgatcc agtgcatcgg gcgaaacggc ataccaaagg tcgttgccat
    20761 tcaataaaag tgaatcattt ttcgttgaat ctatagctat cgaggaatct ggcactgatt
    20821 gtgcagagga atacacaaac gcctcgttag tttgcacatt attatataca aaattgccgg
    20881 aatgagctcc aattgccatg ccgatcgcga atagcagcca tattgtgata aaaaattttc
    20941 gcgacacagg taactttgga atagttttag agtaaatgcg taaatgacag caaatattgt
    21001 gcaaagctat ggaaattatg aagaagcaga tcagtagaac cagtgttatc acaggcgcct
    21061 ttatggtgtt gtggttaggc gtatgcgcat tcaatacccc atcgacaccg gaattgcctg
    21121 cgtacaaatt gaaaactgtt gttatcgacg ccggccatgg tggtcatgac atcggatgct
    21181 ccggtagtgc aaacaatgaa aagacagttg ccttgaagct tgccctaaag ctgggtgaaa
    21241 tgatagaaaa gaactatccc gatgtgaagg tgatctacac ccgtaaaaca gacgtttttg
    21301 tcgaattata tgaacgagca gcaatcgcca atcgaaacaa tgccgatttg ttcatttgca
    21361 tccactgcaa cgccaatccc aacagctcgc cctatggaac tgaaacctat gtgatgggtt
    21421 tacacaaaac agaggcaaac ttaaacgttg caaaacgcga gaatgatgtt atcctgatgg
    21481 aggacaacta caatcagcac tacgatgggt tcaatcccaa tgatccggca tcacatatca
    21541 ttttcagcct caatcagcat gcattcatgg accaaagcat cctatttgca tcatcagtag
    21601 aaaaatatct taaaagtcac gccaaacgca gcagtcgcgg tgttaaacaa gccggtttcc
    21661 tcgtgctctg gaaaactgcc atgccaagtg tgctcataga gaccggattc ctaactaaca
    21721 gcagtgagga gaagttttta gcctctgaaa gcggacaaac aacaatagcc gaaagcattt
    21781 tttatgcgtt ccgcgattat aaaaacgaca tggaaaacaa ctccggaaag gtaagcgagg
    21841 caacagttca agctattgaa gcgaatatgg acaaggaggt tgaaacaccg aaaactgagc
    21901 ctgtaaccac atccacagat aacaaaggac tcactttcag cgttcagttc tatacagcgg
    21961 aatctgaggc ttcggccact aagtttgctg acctaaaacc tgaaaatgtc acatatgtta
    22021 aagaaaacgg tctctacaaa tatcgacttg gtccatacaa aaccctggat gaagccacta
    22081 aaaagcaaac ttttgcccga aatgcgggtt ataaagatgc atttgtgata gctgtgaaag
    22141 atggtaaacg catttctatc gaggaggcca aagccctcgc cggaagctga accaaaggct
    22201 acaacgcaac tattttataa cttagcacgt aaattagcag ggtgaagatc tcgaacgaat
    22261 cgaaggtggg cattttggca gcagttggta tcgcactgtt gtttatcgga ttcaattatc
    22321 taaagggtat taatgtattc agcaaaggca ttgactacaa tgtacaatat gagacttccg
    22381 gaggcttgct cgtgggcgat cctgtgatta ttgacggtct cgccgttggt agagtgaaat
    22441 ctgttacact gcaacaagat caatccggtg ttgatgttat tctcaatttc gaacaacggc
    22501 ttaaaatccc ggcagattca tatgctttga tcagaggaaa cctgatgggt gaaaaatatg
    22561 tgcagatgtt tttgggtgat agttcagaac tcatccctga cggcggcacg cttaccggta
    22621 aaattgaatc agatttagcg aatacaatta gccaggaatt caaacctatt accgacaaag
    22681 tgaaaaccat gctcagctct atggatactg ccattaatgt gttgcgcggt atattcactg
    22741 aggaagtaca aaatgacttc gccaaaagta tggctagtat taaaactact ctggaatcat
    22801 tcaataattc tgcagacaga gtcaatgcat taataaataa ggaagaatca aatattgaca
    22861 gtatcattac cgatgtgtcg ggatttacac aaactgtaaa ccagagcaag gatgaaatga
    22921 cacagatatt ggataatctg aaattgatca gcgattcact caaaacggtc gaatggcaat
    22981 ctttggcagg cgaatttcaa aaggctgctg acaatatcaa tgcaataagt gctaaaatta
    23041 attcaggtga tggcagttta ggaatgctgg ttaatgatca acagttgtac accgacctca
    23101 ccaagacact aacaactctt gactctgtac tcaatgagtt tggtaaagat cctacactca
    23161 ggatcaaatt ctttaaatga tacctgcttc cgacatgcgg aaatcagtgc gttacatttt
    23221 tcaatttata ctggttgcaa caatttgttg tactgatacc atatttgttt acggacaatc
    23281 tgatagttct gctatccaaa ttaaaagcgc cgattatctg gaaattattc aacgaaaaaa
    23341 tgtaagcatc aatcggctgg tacataatgt agtgttaaca cagcgaaatc tgacattata
    23401 ttgcgatagt gcctttcttt ataagtctga aaatatcgcc aggatttatg gccatgtgca
    23461 tctcagtcag agcgatagta tagatgcata cggagattca gcaatatatg atggtaatac
    23521 acgcatggca cacctatttg gcaatgtgca ccttaccgat aattcgatga cgcttacttc
    23581 caaggatttg agttacgata tgtcatcgcg tgttgccact tacgtaaacg gaggaaaagt
    23641 taccgatggc gaagccgtgc tgactagcag aacaggatat tattatgcat tcaccaatga
    23701 tgcatttttt agaaatgatg ttgtgctcaa gcacccgaaa tatcaactcg aagcagatac
    23761 actccaatac aataccggaa ccgaaacggc attcttccat ggcccaacac gtattaccaa
    23821 tgaaaacagt accgtattgt gccgaaaagg atattaccaa acaaataccg gaatcgcagt
    23881 ttttcatgat caggtaaccc tcaataatcc acctcagaaa ttactcgccg acagcattta
    23941 ctacaaccgc gaaacaggta tcgggaaggc attcaggcat atcgtgttta cggataccgc
    24001 tcaacaggta attcaattca gtaacgttgc cgagtacgac gaaaaaaata attcaatctt
    24061 ttctaccgac ggatctgttg caggctatgt gatggaagat gatacgctat tcattggcgg
    24121 cgataccatc agaactgcgc aggatagttt aggtcgcaaa accatgtatg tttttcgcaa
    24181 tgtgaaaatc tacaaaagcg atctgcaagg gctgtgcgat tcattgagtt attctgatgc
    24241 ggactctacc ttacgtatgt acggactgcc tattatgtgg agcgatagca cacaatttac
    24301 tgccgacacc atgcacctgg tgatgctgca tcaacaattg aatcaagtgc agttattttc
    24361 gaatggattt attattaatg aagatgacag cctaatctat aaccaaatta aaggcagaaa
    24421 tatatacgga tatttttcag acaatgacct caaaaaaatc aaagcgatag ggaatggcga
    24481 aagcgtgtat ttcggcaaag atgatgctaa cagatatatt ggagtaaata cgttgtattg
    24541 cagtgaaatt aatatttatc taagcgacaa aaaattccat cgcatcgcat ttaaaaatgc
    24601 accggattca aaattccagc cgatgcaaaa tatcaattta aaggattacc agctcaaaaa
    24661 tttcaactgg cgctacgagg aacggccttc gtcgcttgac gatctgctta aggattaatt
    24721 tggaatcaga tattctccaa cacaaatccc aatcttgcca tatcatacca gaatgaaggg
    24781 tatgatttct ttacaacttc cggttcttca atggtgatag atggcgcaac caaagcaagc
    24841 ggcgcaaaac tcattgccat gcgatgatct tcatacgtac tgatggttgc ttcaacagga
    24901 ttaattttcc ctgataatat ccattcgcca ggctcacgtt cttcaaatgt tacattgaat
    24961 tttgccaatt cagattgcaa ggctgctgtt ctatccgttt cttttatacg caaactttcc
    25021 acgccggtaa atcttgcagg tatacccaaa gcagcacaag taacagcaac tgtttgtgct
    25081 atatcgggac aatcactata atcctgatca agcaaggagg tgcaatttcc ggtatttgaa
    25141 attatcattt cctttccttc gaaaaatgtt gtcacgccaa gcttagcata gatatcagga
    25201 agcacgctat cgccttgcaa actcttttca aaaagaccag atatgcgcat gtgtgatccc
    25261 ggcgataatg cgcaaatact gtagtgatat gatgcagcac tccaatcgct ttctgcctgc
    25321 atagcaacag accgatagct gccatttttt atcgtaatag ttgctccttc ccatttgcaa
    25381 tcaataccaa gctcctgcat ggtgctcaag gtcatttgaa tatacggcgc ggatgcaatt
    25441 ttcccatcaa gaatcagtgt gagacctttt ttcaatgtag gtccaatcat catcaatgcc
    25501 gaaatatact gcgaactgat atctgcccga atgcgaatgg ttccacccgg taattccttg
    25561 ccttttattc tcacaggcgg ataccctttt ttctcaatgt aggtaatatc ggctcccaat
    25621 ttttctaaag cctccaccaa tacaccgatt ggtctttgct gcatgcgtgc actgcctgta
    25681 agcatgcgtt caccttcaag tgtgctgtaa tatgctgtca gaaaacgcat ggtggttcct
    25741 gctgcaccaa catcactaat ggaaggttgt tgcttcaata aacgctgcaa tgtcgttgta
    25801 tcgtcagaag gggataaatt gcgtatttca aatcccgaag tgcataatgc ctgcatcaac
    25861 aacaaacgat tgctgatgct tttacttcct tcaagtgtta ttgcactgcg aataggctgc
    25921 agtggcgcct ttattttcat cttcatcaca aaccacaata aaattgtaag gctgcagcaa
    25981 tctgatcatc ggtagcaacg agatcgtact gacacaaacc aatatgctgc aataaactaa
    26041 atcgtatttt ttgttgcgaa ttctttttgt cgagttgcat gatatccatc aatgcattta
    26101 cagacacttt ttcaagcgga tattttccat accgatgcat caacatgttc gtcacctcca
    26161 ttaattcgct ttcagagaaa ccacaaatca ctgtcgagag ctgtgcctcg catatcatgc
    26221 caatagctat cgcttcgcca tgcaacagcg gatcttcatc gtttaataaa ctccaggttt
    26281 caacggcatg gccaatggta tgtccgaagt ttaatatttt gcgcaacccc ttttcatagg
    26341 gatctttttc tacaacatct ttttttattc gaacagaccg ttcaatcaat tgctgcacca
    26401 gcgatacatc atgcaaatct gcattatgca atgtccggag ataatctgca tccgcaataa
    26461 acccatgctt caacatttcc gcgaatccac tttttacttg cctttctgac agagtggtta
    26521 aatatgcagg cgaaataaaa acagctttag gatcgcgaaa cacgcctaca aggtttttta
    26581 cgccatcgag gtcgattcca agtttaccac caacagaggc atctacttgc gataataagg
    26641 ttgttggtat ttgaataaaa tcaattccac gtttgtatgt tgatgcagca aatcctccca
    26701 tatcgccaat cactccacca cctaaattta tcaataagct ttttcttcct gctatacatt
    26761 ttgcgagcga ctgccatatg gtaatacagg tattaatatt tttatatgcc tcaccggaag
    26821 gtatggtgaa aatttgatgt tccggcaata aaggtttcaa taaaggatag caatgcttct
    26881 ctgtttcacc atcgcaaagc acaaataatt gctcatattg ttgcgcaatt atccaggctg
    26941 aaagggcacc atgatgttca ggtgaactgt cgatataaat tggataggaa gctgtttgca
    27001 ataccattat ctaatttttt caactgacca ggcatcgggg cgttcagaaa acaacaattt
    27061 tgttgccgac caggttgttt taaataattc tgatttgcga tattcatcca gagatgccag
    27121 attttcccaa atactaatgg tcataaaaat gtttgattgt agtacatctc tggtaaggtc
    27181 aacacctttg cagcccggaa atgcttcaat cttgtgctgt gcgtccgaaa atattcggag
    27241 aaaatcttca acctttcggt ggtcgaaggt catttttaca atgcggatga tcattcaaat
    27301 tctatttgta tggtgttatc taaatggagg cctaacaact gacttgcatt tcctttgtgg
    27361 atggcgattt ccagatagcc ggaagaatta aataaggcca gtttctcccc ttccggcaca
    27421 tctgtatatg tggatgaaat ttcgcgaatt tcttcggtac gtttataatt gatcacaaat
    27481 tctcttcctc cacgcaattt ttcaaaacgc tcctttgtga tgttgatgat caggtttcca
    27541 aatctatcga tataagctat gtttgcccta atgagatatg cattatccgg cgggcgcaaa
    27601 taagtctttg tttccagcga tcccaaagga gtgccgattt gttgtaaagt tttaccttgc
    27661 aataaactga tagcagcatg cactaatata tctttcgcag caaacgaaga ttgttgtcca
    27721 tgtgaaatag gtatttccac aatctggtcg ggagcatctt ccaccataag tgaaaacaat
    27781 ccattatcca taccgataaa gtactgatca cgcatgcgaa tggcaacagc cttaacggga
    27841 ctgcgagatt cggcatgaac gcttacaaga tgaattgtag acggcgggaa atgaaaacat
    27901 gcattgcgca ttacaaaagc agcctcctga atattaaatg gggcgatctc gtgggaaatg
    27961 tccacaatca aggcttgtgg tgcgcctgac agtaatttcc cctttaacac tgccacatag
    28021 taatccttta caccgaggtc tgaggtaagg gttacgatag gcataaatgg gaactaaggg
    28081 ctgctggtta gttcaaaaaa ggtaaatttg catgaacgtt caaaattacc aataaaattg
    28141 attgaagtaa ccattccttt agacggtgtg cggccgattg acttctatgg tatacacaat
    28201 gcgcgccttg gcatcatcaa aaaaacattc ccatttctca atatctccgc ccgaggtagt
    28261 gagctgaaag tgcgtgggaa tgaaaaagat atcgaagatt ttaaacatcg catcgacctg
    28321 gtcatcaacc acctcgaaaa atttaatact atcagcgaca gcaatctgca aagcatctta
    28381 ttcggcgtgc ccgaaactga gcttgtcgat ctgcctgaat tacctgatga tgtgattgtt
    28441 cacggtccat ccggttatct ggttagagca cgcacaccta accagaaaaa aatggtaagc
    28501 ctggcaactg ccaatgatat tgttttcgcc attggcccgg ccggaacagg aaaaacgtat
    28561 accgccgttg cactcgccgt gaaggcattg aaggagaaag ttgtaaaacg catcatcctc
    28621 acacggcccg ccgttgaagc cggcgaaaat ttaggatttc ttcccggtga tctgaaagaa
    28681 aaaatcgatc catatctgcg accattatat gacgccctgg atgatatgtt gccgatagaa
    28741 aaactgaatt tatacatgca aaatcgcgtg atagaaattg cacctttggc attcatgcgc
    28801 ggaagaacac tcgaccattc atttattatt cttgatgaag ctcagaatgc tacagatgca
    28861 cagttgaaaa tgtttcttac acgcatcgga cccagcgcaa aatgtattat taccggcgat
    28921 cttacccaaa tcgacttgcc gagaaatcag caatcgggtt tatttacatc gttgagaatt
    28981 ctgaaagata tcgatggcat tggcaccatt ttcctcaatg aaaatgatgt tgttcgtcac
    29041 cgcctcgtga agcaaatcat caaagcatac gatgtagaaa aatcgcggca ggagcaccac
    29101 ggcgagtgat aattaaagca gcattaggat tgataaccgg caggtaataa tccatattct
    29161 ttccactcat caatcggaag cggaagttcg tttattaagc catgtgcaat atccaaaacc
    29221 catccatgca attttggata ggcgccttca ttaaatgctg cgtgcatcac gcttgttttg
    29281 cataattggc gcaattgcct gattacattt aattccgaga gtctgtttaa cctttcctga
    29341 taatcttcaa tcgcgtcgag ttccttttta tactccatgg caatatctct gatattcatg
    29401 gtccagttct ctacaatatt catggtagtt cctgaatagg cagcttccac cccaccacat
    29461 ttataatgtc cacagatgat cacatgttca accttcaaca ctttcactgc gtactccagc
    29521 accgacaaga ggttcatatc atttaggaat acctgatttg ccacattcct gtggatgaag
    29581 attttacccg gatcggcacc ggtaaaaagg tcaattggaa ggcggctatc gctgcaaccg
    29641 atatacagaa atggtggatt ttgcccaact gacaaccgac caaaataaga cggatcttct
    29701 gccaatttct cggttaccca atccttgtta ttgatcaata actgctcgta ggtggtcttg
    29761 ttttccatat gataatggtc aaaagtagta taaacagact ggtttttcca gtgtcaggtg
    29821 aaaatagccc ctatcaaggg ctgttaacat tcataaaatc ttgaaaatgt taaaatttaa
    29881 cgccgattga acattttata agaggcactg tttcggacaa aatcacacat atgagcggtt
    29941 tccttgaccc tgttgtattg ttcttcattc tcggtgcact ggcaggcgtt ttaaaaagcg
    30001 atttaaaagt cccggaagca ttttatcaca ctttaagtat ttacttgtta ttagccatcg
    30061 gcatcaaggg tggtattgaa ctttatcatg caaacccttc gggaatttta ttgccggcaa
    30121 ttgcaacgat aacggcagga ataacagtaa cactgatagc ggctttattg ctaaaacgta
    30181 ttgcaaaatt caataatccg gatgtatggt caattgcagg tcattacggt tctgtgagtg
    30241 cggtaacatt tgcagtaatt ctcacattcc ttaatgataa acaagttcca tacgaggaat
    30301 tcatgacagt cctgttggtt accctggaaa taccggcaat tgcgtttgca atttttgcat
    30361 ataagtcatc tgtcagttca ggcaaaattg aaattcgaaa gttagcacat gaagtattct
    30421 ttggtaaaag tattttattg ctgcttggcg gattaggcat aggatattat acggccgcag
    30481 caaataatca acaactcaac ttttttttct ttgacctatt caaagggttc cttgcaatat
    30541 tcatgcttga aatgggaatt gttgcgagtg cacgattcgc tgacttgaaa aaagttggca
    30601 tttcgctaat actgtttggt gtaatgatgc cattgattgg ggcatcaata ggaattctcg
    30661 caggaaatat tgtaggcttg tctgtcggag gaactgcagt cctggcaacg atgtgcgcaa
    30721 gtgcatcata tatagcagcc cctgcagcaa tgcggattgc tataccggat gcaaatccca
    30781 cttacagcct tactgccgcg cttggtatta ccttcccttt taacattatt ataggtgtaa
    30841 acctatatta tcgaatgagt gttatcgttg atgggatatt gaattaatac caatactttc
    30901 ttttcaaatt aaatactttt ctttaaacaa cttcatgtga tgcagctcat gacccacaat
    30961 gataaagcca agtgcgttca ccgatatttc cttgccattg cctgttccgg tgcgtgcaat
    31021 catatcgtct gtaaaagatt caaataacat tatggtagca tgacgcaccg ctgcaaattc
    31081 ctgtaacaaa gaaaccatgc tcctgttatc ggccattgat accggagcat aatcattttc
    31141 ctcaaaaccg ggtaaaggag ttttgtcttt gcgggcaaat cgcaaagcac gatagcacat
    31201 aatgcgctct gtgtctatca gatgttgcaa cacctctttc acgctccact ttccgggggc
    31261 ataacgaaac aaggccttct gctcatctat ccctgcaagc atgtcgatgg tctgttgaac
    31321 atgttgcgac aacatctcaa gcacatcatc tccttctacc aaggctatat atttttcctg
    31381 atatggaaat acttctccgg gttgtggttt tttccaattc gccatttttt caacttttat
    31441 caatgatcac aaaaatgaat ttctttcggc aaagtaacaa gcatgatcta aactattatt
    31501 gttaaaatcc ttccatctct ataggacacc aatctcccgc aacgcttatc tttgcggcat
    31561 gaaaaaggtt ttcgactctg tgaaaacagc acttggtgaa acaacaggtg tactgggcga
    31621 agggtatctg gcagcaacag acttactaaa ggaaacatta tccaacttct acgatgtgaa
    31681 agacctcacc aaagagaaga tggcttccat caccaacgat gttatagcac tggctccaat
    31741 aatcgaacaa accggttaca gagcaaaaga aatcaatatc ggcgttggca taccacctcg
    31801 aatggtattt cacttcgaaa aatttgccga catcaaagat gaagaaatca agcgtatact
    31861 cgccgaaaat aaagacaaaa aattgcttag cgtgctggta aataccttag tatcagcaga
    31921 tgcatttcag caaaagataa ccttgggcag tttttcattt aatgagatca gcatagagtt
    31981 gagtgtgcca cctgaagtaa atgttaaatt tgtgaacgca gccacccaac aaactgttat
    32041 ctgatcatgg acattatttc gctgcttact cgactggcat ttacagccat attttatttc
    32101 atcccggata ttttcatcca tggcgccgta aaggcctcgt ttacaaaaaa tccgaaacgg
    32161 gcattaaaaa gatattggtc actaaatatt gcgcttttct ttattacgag cagcggattt
    32221 ctgctcatgc aatttatgag tcccgacttt gccaggatca atctgtactt tttcggtata
    32281 ttcgtaagcc tcttatttgc caaactcata gtagcgataa ttttattaat cgctgaggat
    32341 atctggcgca taccttacag tcttttcaca cgaaaaaaac gaaatgttga atctacgcct
    32401 gaaaaagcac cattgctgat tagtcgcagg aaattcattg caaaatccgc actcattgcc
    32461 ggagccttac cgtttaccgg atttatgtat ggcattttta aaggcagata tcgatttacc
    32521 attcatcgcg aaacattgta ttattctaat ctccctgatg cattcgatgg atttaccatc
    32581 acacaactca gcgatctgca tatgggttca tgggatacca acgcgaaata ccatattcag
    32641 actttggttg atgaggcaaa tgcactaaaa tctgacttaa tattatttac cggcgatatc
    32701 gtgaacagca gagctgatga aatgtcggga tggtatgaaa tagtaaaaca attacaggca
    32761 cctttcggta aaatttctat ccttggcaat cacgattatg gcgactatgt gaattggaaa
    32821 acgcctgagg caaaagcggc aaatctggca ttggtgaaaa atattcatcc tgccataggt
    32881 ttcaatttat tgttaaatga aaatacatca atagaaaaag agggagccaa aatctatatt
    32941 gcaggtgtag aaaactgggg gaaaggtgat tttccgaagt tcggtgatct cgacctcgca
    33001 tataaaggaa ttcctaatga tgcattcatg cttctcatgt cgcacgaccc atcgcattgg
    33061 gatgcaaaaa tattggagca tccgcgttta ccaaatctca cattgagcgg gcatacacac
    33121 ggatttcaaa tgggtataga agtgcctgca attcgattct ctccctctca atttatttat
    33181 ccgcaatggg caggactcta taccaaaaaa gatacgcata tctacgttaa tcgcggactg
    33241 ggaacagtgg gttatccggg acgtttgggc atttggcctg aaataacaca aattacactc
    33301 cgtaaaaaat aaattgtaat tttaaaatat gaaatatatt attcctgtta ttctgctcgc
    33361 agttaccttg tcttgcgaaa aaaaagaaaa acaatttgat gcagatggcg tcttgttggc
    33421 acagtttctt gaatcagatt ccggaagcgg acttgacttc agtccgatgc aaaatgacga
    33481 tagttcaagg gtgatcaatt ttacgcccac catttacaac tttaagaaaa aaccatatac
    33541 gggaaaagtt actgcgtatg acgacaagca acgaaaaata ttcgacggga atttaaatga
    33601 tggtttaccc gaaggcaact ggatatttta ttacgcttca ggtgttgttc agattgaagg
    33661 cacttataca aaggggttgg aaacaggtat atggaaaaat tattataccg tagacaaacc
    33721 aaaagtgatc aaacaatatg atcaatccgg atatttattg atgcgcaaag aattttacga
    33781 tacaggcaaa ttgaagaact accaaaatgt aaaatgtccg caatatggcg atatcgctcg
    33841 tcgtgtgcag tttaaataca atggtgccat agagtatctg gacgctgaga gagaacttgg
    33901 caaattagac ccggctgaat tgcaaactgt attgttgaac gacaaattga tggtcaaata
    33961 agccgacctt acttcaacat aatttaatta ccatttctgc gttatgggtg aaaacccaaa
    34021 ttaatgcgca cgctccttct ctttgtaata gcttgtagtg ttgctgctgc atgtgaatca
    34081 tctgacggga tgacaaccga cgaaacagat gaaatagtcg ttaaggaggt caatatcgat
    34141 tctatagacc actgctattt agtgggtaag caagccgaag ccaaagcatt ctgtaaatca
    34201 gaaggatata ataagcatta ttgttttcta cttgacatga gccgccattc gggacgctac
    34261 cgatttttta tatgggattt tgatagcaac aaagtagtgg acagtgcact ggtaagtcat
    34321 ggttgcggta taagcaattg gggtgatgat ggcactgctg atgcaccggt tttcagtaat
    34381 gaatatgaga gccactgttc atcattagga aaatatcgcg ttggcgaaag agcgtggagt
    34441 caatggggaa ttaacataaa atatcaatta tacggtctcg atagcaccaa cagcaatgca
    34501 aacggacgca ggatagtatt gcatagttgg gaatttgtta acgacattga agtatatccg
    34561 gatggctcac cggaaggatg gggttgccct gcagttagta atgcatttat ggcacgactg
    34621 gatacccggt taaaaaaatc aaccgaacct gttttactct ggatgtatac gcattaatta
    34681 acgcatcaaa tgcaatgctc ctgaataggt gcgattttct ccatcgcaaa gcattttgat
    34741 cagatacaca tatgtaccga tctcttgagg aattccgccg gaggttccat cccatccttc
    34801 agaatcagtt gtagcataat aatcatatac tacctgtccc catctgtcga atactttaaa
    34861 gtattggata tcctcacaca aattcgctac aggaggtatt ctaaagacat cattgatacc
    34921 atcatcattt ggcgtaaatg catttggaaa agctactcct gcggctacta aaatggtaat
    34981 gcaatctgtt gaagaacaac ctgtaggatg cgtggccgtt acacagtaag tagtagtttc
    35041 atacggatta ataacaggat ttgcagcagt tacatcgtcg atattataat cgggtgacca
    35101 taggtaagta taggtatcat caagattaga atacaaggtc atatagttgc ccatttcgag
    35161 cgatgtatcg gggaaagcgg taatttctac tgtatcaata acatctacca ccacttcacc
    35221 gattagctga catccaagat catcttcaat gataacagta taggtcatat ctgtttcagg
    35281 aatgacagta acatcaggag tagttgaagc atcttcatca ttccagtgat aactaacaga
    35341 tccggttcct gttgcaacag cactcataaa taattcattt ccctgacaaa caactgcatc
    35401 atcaggaatc atatcgagtg taagttcaac atataattga tctgcaattg gaagtgcaag
    35461 gccaccgaca tagtttccgt ttgaaccggc agttgcgcca ttaactgagt taaaacatgg
    35521 tgaagctgcg ccttctccaa cagtaattcc tgaagaacca ccgccggcat caaggtttac
    35581 gtttggaggt actgcaccgg aaggcatcca aagaacacca ccagagcccc cgcctccggg
    35641 acccacgcag ttaacgccat caaaggtatt atctacatca ccaccatcgc cgccgtgaac
    35701 ttgaacattt actactgaag agattgaggc tgcatcaata agcacagcac ctgctccgcc
    35761 tcctccacct gctccgtcat caaaagcagt tgtggttacg gacataccat ttgatcgtat
    35821 aaagaaatta tttccctcaa tattattagc tataattata atgataccac caccattacc
    35881 acccgaagta cctgtgccaa ggtttgaact accctgacct ccgccgccac ccataaagat
    35941 gcggttttca gcattgtcat aaatgagcgg attaccccct attcccgggt atgaaccacc
    36001 acatgaacct gcaggaggga
//
LOCUS       DAOQHY010000011        34906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_497_length_34906_cov_4.890477, whole genome shotgun sequence.
ACCESSION   DAOQHY010000011 DAOQHY010000000
VERSION     DAOQHY010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 34906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34906
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_497_length_34906_cov_4.890477"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="PLL28_01835"
     CDS             complement(<1..903)
                     /locus_tag="PLL28_01835"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF68082.1"
                     /translation="MKNVFTIFKKEIKDILRDKRTLTVMLIIPLFVYPAIFVGISKYV
                     AGQTEKAGQETLRIAIIDNGNANHFSKFVAGMPNTSIKQIADTTSAQTMINNDSIDVA
                     YYFPAGYDSAIAKLEPTTFEYFYTSTNNEFEVAIIENLNNAYKQQLIKEKLEDNHLSG
                     TILEPAKPVSRNLASSREQIGSMIGGILPYFFIIFCMIGCMYPAIDLAAGEKERGSLE
                     TLLVSSASRLEIYLGKLITVTLSGFISAMASIAGIIMSGKLIGADASQDGGIAQIADL
                     LNGIMEPGSIAMMLLILLPLNIFFA"
     gene            complement(924..1706)
                     /locus_tag="PLL28_01840"
     CDS             complement(924..1706)
                     /locus_tag="PLL28_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNF68083.1"
                     /translation="MITVDKVSKSFSLSRQQRKELGADHSGKTVNAVNEVSFVCQPGR
                     IFTLLGPNGAGKTTTLRMIATILKPTSGNIIVNNYDTVTQGEQVRGSLGFLTGSTGLY
                     DRLTPNETIDYFGRLNGMDEKTLQDRKKYLFDLLGINEFADRRVGKFSTGMKQKVSIA
                     RTMIHDPQVVVFDEPTSGLDVIAAKSIIELIRQCKKDNKTVIFSTHIMSEVNMLADDL
                     AIIYKGDLIYNGLFTDFKAGMRETTLEDEFIRIAQEHENVTA"
     gene            complement(1712..2590)
                     /locus_tag="PLL28_01845"
     CDS             complement(1712..2590)
                     /locus_tag="PLL28_01845"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNF68084.1"
                     /translation="MKSLIKLTSLLTLLPFALNAQQGAKSTSILYAISGNGLTDTSYL
                     FGTFHLINQEHFYVAPEVETAFDNAAQLAFEIKLDDPNMISTYQRWTTLPEGVTWHNF
                     ASDEEIAYMTSVFRDSMQADFTPYIQLKPFVAYQMLTTESMGENAQSYELHFMQRNMQ
                     DHRPIAGLETLESQLKIFDSVPYAEQVDWMTEISDSSFTSEDRLDEMISYYKNGDIES
                     LYNVTITATPEFHDYANLLVADRNKRWIPIIGDLAKKGSTFVAVGAAHLGGPEGVVKL
                     LQQAGYQVTPVKLTEK"
     gene            complement(2587..4410)
                     /locus_tag="PLL28_01850"
     CDS             complement(2587..4410)
                     /locus_tag="PLL28_01850"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNF68085.1"
                     /translation="MQQEQIPLEDFFKNPEKTGFKLSPGGKRISWLANYEQRLNIYIR
                     DSENNIEKRLTARTDRDIRSYFWLTDDQLAFFQDTGGDENEHIFVVDVHTGDTKDITP
                     FEGVKVDLIDELEDVDDAIIIGMNKRNPEIFDVYRLNTITEAITLIEENPGNITAWIT
                     DHTGALRLAMTTDGVNTSLLERTAPGQFITIITTGFRETLAPLFFDFEQKHVYALSNI
                     GRDKIAIVLIDITDAHEIQCLFEHPDVDAGQLGFSKKRKVITSATYTTWKTEFVFFDS
                     ESEQRYAKVKSLINEDAEIYITDRDKAEEVFIVRTLSDRTLGNYYLYHATNNTITHLA
                     NVSPWLQANKLCSMEPISYESRDGLTIHGYLTLPDSGHAPYPVVIHPHGGPWARDVWQ
                     FNPEVQFLANRGYGVLQMNFRGSTGYGRAFLEASFKEWGKKMQDDITDGVQYLIEKGI
                     ADKDRIAIYGASYGGYATLAGLTFTPDLYTCGVDYVGVSSLFSFMETIPPYWAPYLEM
                     MYEMVGHPEKDRELLAEASPLLHIDNIKAPLFVAQGARDPRVKKSESDQIVQALTEKG
                     VDVLYMVRENEGHGFMNMENKFAFYAEMESFLSRHMQSKKK"
     gene            4555..5214
                     /gene="rpe"
                     /locus_tag="PLL28_01855"
     CDS             4555..5214
                     /gene="rpe"
                     /locus_tag="PLL28_01855"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNF68086.1"
                     /translation="MNPLVAPSVLSCDFAYIARDMEMINRSAADLVHVDVMDGVYVPN
                     ISFGLPVVAAMKRHSTKPLDVHLMMVNPERYLQDFRDAGADSISVHIEACTHIHIAVQ
                     QIHKMGAKAGVAINPGTAVSALEDIIRDVEIICFMSVNPGWGGQSFIETSIEKIGRLK
                     ELILKTGSKALIEIDGGVKLDNAQRIFDAGADILVSGSGVFGQPDPVAAIASLKSLTR
                     Q"
     gene            5243..7723
                     /locus_tag="PLL28_01860"
     CDS             5243..7723
                     /locus_tag="PLL28_01860"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNF68087.1"
                     /translation="MFRIPLLMALVFAGNLLHAQGKATVYGNVRDEENNPLPEANITL
                     LQPNTTIQTDKQGNYSFEVPAGDTIVLAFTFVGNEPHYEKVILEEGQRYLLLVKLKSI
                     TLGTVVVSDPKETIFRIPVRELEQIPSASDPISAILTALGAVKNNELTSQYSIRGGNY
                     DENLVYVNDFEVYRPLLVRSGEQEGLPFPNYDLIDNIAFSAGGFEAKYGDKLSSVLDI
                     QYKAPREFESGLTASLLGASYYIGDAISTDRDSLIDSTKGYYLFGARYKTNRYLLSSL
                     NTEGQYEPTFIDGQGLFGWKFNRHSSIEALGNFSLNSYTFLPLSRSTATGVVNDVVQL
                     DVFFEGQEIDKFITGFGGFSYRYQRDSFTVYKFLISGYHSLETETFDILGEYWLGQVE
                     TNLGSEEFGNVLYGLGVGAFQNFARNYLSSDVFNISHLGTREYNTHYMQWGVRAQYEL
                     ISDELKEWELLDSAGYSLPYTGETVQLQEVFNSSTTLNSLRYSGYWQDTWEPDSAHIS
                     LTAGVRSSYWDLNNEVTITPRIQFVWQPQWKNKLNQRRNIELKASTGLYYQPPFYREL
                     RDLEGNVNTSVKSQKSLHVVIGSDYKFKAWNRDFRFITEMYYKYMFDLIPYDVENVRI
                     RYYGENMATGYAAGIDLRLHGEIVEDADSWVSLSFMRTMEDIEGDSTVTVLDKDSDID
                     SLLYSPRGYIPRPTDQLVNFGMFFQDYMPGNKNFKVHLSFLIGTGLPYGPPNNEYFRN
                     SLRIAPYRRVDIGFSALLLSPKRDLIGKPWYNTTFESLWASVEVFNLLGIQNTISYIW
                     VKDVNNLQYAFPNYLTDRRINVKLVAKF"
     gene            7759..8154
                     /locus_tag="PLL28_01865"
     CDS             7759..8154
                     /locus_tag="PLL28_01865"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNF68088.1"
                     /translation="MMQRLICVLFIFYCGQAFGQQVDINALQPDSVYENVCSKKIYSD
                     SLSTTFVIWIKHTVPKHYHAAHTEQVFVLEGHATMLLGDVWLDVAPGQQIFIPKGTPH
                     SVKVKEGEVLKVISIQSPMFDGTDRIKLE"
     gene            complement(8161..8622)
                     /locus_tag="PLL28_01870"
     CDS             complement(8161..8622)
                     /locus_tag="PLL28_01870"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HNF68089.1"
                     /translation="MWRFLTFLAINFGALGIGSLLMGSIPNNTWYNDLNKAPWTPPGW
                     VFGAAWFTIMLCFSIFLYIVSGKYSAAVLRPLYLLFAIQFVLNVMWNPIFFRWHMVMP
                     GLVVILSLTIIVALIIRWGFTNHSQATWLVLPYLIWLCIATSLNAYVLLKN"
     gene            complement(8669..9595)
                     /locus_tag="PLL28_01875"
     CDS             complement(8669..9595)
                     /locus_tag="PLL28_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF68090.1"
                     /translation="MENRKQTTILLIEDNPADARLVEIYLNESAMIQPQISRVSELGK
                     GMLMLEERDFDIVLLDLTLPDSSGFETVKTILREFPGQTVIVMTGLEDDTVAINSVKA
                     GAQDFIVKGQFDSNLLGRTITYAIERHRLQIKLEDYAKAIKLNEQRLTEAQKLAQMGN
                     WELDVVNNQMYWSDEVYRILGYKQGQLEPTLADYLKPIADDDVEEVKAILNRTLEKGQ
                     PFHAEYKVKGPGNSTKKVSIQGLVQKSKINGGLCLAGTIQDITNLSLAKSEGIDKQAL
                     MSVLKNAIAEADNSGASASLKKLLESGLNMIK"
     gene            complement(9605..10057)
                     /locus_tag="PLL28_01880"
     CDS             complement(9605..10057)
                     /locus_tag="PLL28_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF68091.1"
                     /translation="MRSHNEDVMQILLVEDNPGDIRLTQEAMKEGPFKSELHVVKDGV
                     EAIEFLRHKGKYSLSPVPDIILLDLNLPRKDGREVLAEIKNDEVLKLIPVIILTTSDA
                     EQDIYKSYKLNANCFITKPVDLDQFIYIIRQIETFWFTVAKLPGRRSA"
     gene            complement(10149..10835)
                     /locus_tag="PLL28_01885"
     CDS             complement(10149..10835)
                     /locus_tag="PLL28_01885"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HNF68092.1"
                     /translation="MPKRILFILLFTVTLGYAFSPAPATAPTYSIALLKYNGGGDWYA
                     NPTSLPNLAEFCNKNLNLNIATDIPTVEVGSPELFNYPFVHMTGHGNVVFSAAEAENL
                     RTYLIGGGFLHIDDNYGMDPYVRPAMQKVFPELSFVELPFSHAIFHQHYEFRTGLPKI
                     HEHDNKPPQAFALFWEGRMVCLYTYECDLGDGWEDRDVHKDPEETRQKALQMGANILQ
                     FVFMENTPNG"
     gene            complement(10828..11538)
                     /locus_tag="PLL28_01890"
     CDS             complement(10828..11538)
                     /locus_tag="PLL28_01890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNF68093.1"
                     /translation="MHCAYLPDYFTGDTAELPDVEIHHLLHVLRADIGTKIRFSNGKG
                     LTGNADITLLNKKQGHIHITDATHHQRLSPSLHIGVALLHNSDRMAFLMEKLTELGVD
                     RITPLLTSNTERRHFKRDKELSHMVAALKQSAQPFLPQLDDVVQFLQWLPQVSKTADR
                     RIAHCRKTGMIPLAKTCSENAICIAIGPEGDFSEAEVAAAIDHGFIEVSLGNEVLRAE
                     TAAMSAVMTIKTWRTLHA"
     gene            complement(11545..12408)
                     /locus_tag="PLL28_01895"
     CDS             complement(11545..12408)
                     /locus_tag="PLL28_01895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNF68094.1"
                     /translation="MKRIFTLSICTIISAFLHGQLPVFSEHAITPSSINPALTAYHSD
                     ALIHAHYYNTFSGAIDNATTANIIGEYSFDKNKAGIQLMHDNFGVDIQNIVSLNYARH
                     FKVANGTISGGVSFTGKNIARELSKTHPASSGDPTLQADISKTLFNIGAGVAYITNDA
                     FIAIGTPGFMQENIAEHDNNTDASSEIPLFINGAYRIPLNDTWNITPAVFTALENGQP
                     LILDLQAAITWHEHLKATIGYGNDHSWLAGIHARFNDRYTLGYIYDMHLPLGVASHEV
                     MLGFDLNSSIF"
     gene            complement(12405..12758)
                     /locus_tag="PLL28_01900"
     CDS             complement(12405..12758)
                     /locus_tag="PLL28_01900"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNF68095.1"
                     /translation="MKLNGLFLVSILCVVTNTVIAQTACADPVIPDVITPNGDGINDK
                     WEISCLAYFPDNHLTVYNRYGKIVYEADGYDNNWDASWNIDKGPLPDGTYIYLFVVNQ
                     TDNKKTYTGSLTVAR"
     gene            complement(12755..13558)
                     /locus_tag="PLL28_01905"
     CDS             complement(12755..13558)
                     /locus_tag="PLL28_01905"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNF68096.1"
                     /translation="MPKIAWISALCVVAVLATGFTPPHHGKTGRFLKRHGFMYIPGQA
                     DGMNHDFYMQAYEISNENYRYFLNDLQIKGRYDELRIAGVDTAQWDAIFGDSSAYSKF
                     YFQHEAYDHYPVVNISYEGAVMYCKWLTEAYSLRGFNIEVRLPDSTEWKIAARGGEAD
                     NTYGWGKDSYISKQGYYRCNAKKGDTCSLTLTPVMAYTTNRYGLYNMSGNTTEMLLEK
                     GKHKGGGWNSPEPNIRIDGPDDYAGISGPSPFIGFRPIAIIHDEKTTAK"
     gene            complement(13643..14455)
                     /locus_tag="PLL28_01910"
     CDS             complement(13643..14455)
                     /locus_tag="PLL28_01910"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNF68097.1"
                     /translation="MKYLTSTLLILCLITLTTSFSPQKQAKVMKKQGFVFVPSGATTV
                     DGINLTINGFYMQAGEVTNKEYRTFLEDLKAKGKTAEYEIANVQSEKWILPFSSLEEM
                     KEHYFTHPAFDNYPVVNISQQAARLYCTWLEEQLKANGVGAKVRLPEKAEWVYAAHGG
                     IQGSIYPWEGNELRNKKGIYLANYKTSSLSEDGAYMTAISKSYFPNGFDLYNMSGNVS
                     EWISTDGETKGGNWWSEPEYLRIDAAQEFPYSTEASPFIGFRTVVTVTSLVK"
     gene            complement(14551..16161)
                     /gene="pckA"
                     /locus_tag="PLL28_01915"
     CDS             complement(14551..16161)
                     /gene="pckA"
                     /locus_tag="PLL28_01915"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HNF68098.1"
                     /translation="MESGIKNPAASLAAIGLNHTGHAFWNLSPAELVEHTLQRGMGVM
                     ADSGALVVDTGKFRGRAPKDKFTVKDAKTTDTVDWNNFNIPTDNATFDKLYAKVLAYL
                     SDKDIWVRDNYACADPKYRINIRVINEYPWGNMFCDNMFMRPTAEEIKTMNPDWLIIN
                     APGFQADPAVDNTRDKNFAMIDFTKRIILIGGTGYTGEMKKGIFTVLNYWLPQEKGVL
                     SMHCSANVGKEGDVAVFFGLSGTGKTTLSADPDRRLIGDDEHGWTEEGVFNFEGGCYA
                     KCVNLTRENEPQIFNAIKFGAILENVEFIPGTRTVDFTNITKTENTRVSYPIHFIDNI
                     MVPSKGGLPKNIFFLTCDAFGVLPPISKLTPGQAMYQFLSGYTAKVAGTEAGVTEPQL
                     TFSTCFGAPFLPLHPTKYAELLGEKMRKHNVNIWLINTGWTGGAYGTGKRMKLSYTRA
                     MITAALNGELDSVSYATHDVFGLHFPTTCPNVPTEVLDPRNTWSDKQAYDAKANNLAA
                     QFVKNFEKYADKANAEIMAAAPKAGVSA"
     gene            16400..17044
                     /locus_tag="PLL28_01920"
     CDS             16400..17044
                     /locus_tag="PLL28_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68099.1"
                     /translation="MHKSPRILLSFAFFLMVSGVYAQAFSTLKLTSLHNYEIVDDAFN
                     RSLVGLSPEIGYEAYNYSYQLEIAAYIPRQIDSMVYVYDSLFGYGFDAPVSETMTIFT
                     IGLMSRYYLFSDWMPVNRLYTVSGIMGVFEHVNQDFIAGGLSEGKKFTNKSLDLTFGL
                     GFETLIDDYLMPFIETSVYFPVAQGENPYNTVYDITEGYGVRIAGGVKLYLSDM"
     gene            complement(17114..18220)
                     /gene="dprA"
                     /locus_tag="PLL28_01925"
     CDS             complement(17114..18220)
                     /gene="dprA"
                     /locus_tag="PLL28_01925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNF68100.1"
                     /translation="MYHLIAISLIPGIGPVLARNLVSYCGSAEAVFKVKRSHLLKVPG
                     IGEAVADNIRRFSDFDRVEQELRFIEKNEVQCLTYLDELYPRRLKQCPDAPLLLFSRG
                     NADLNAPRILGVVGTRNATSYGKGICEDLAASLKDENILIVSGLAYGIDIAMHKACVK
                     NNVPTIGVMATGLDRIYPPAHRETANKMMTNGGLLTEFMSDTNPDRQNFPRRNRIVAG
                     MIDGLIVVETGINGGAVITALLADDYHRDVMAFPGQIHHPYSTGCNKLIKSNKAAMVE
                     STEDVLDIMGWSSNTTKTQHVQQQLFHDLNDDEQQIHTILQEYGELYIDDIQSKSQLT
                     PGNLAGVLLSMEFKGLISALPGKRYRLERVAIQM"
     gene            18278..21607
                     /locus_tag="PLL28_01930"
     CDS             18278..21607
                     /locus_tag="PLL28_01930"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNF68101.1"
                     /translation="MRHYQTIFVTFFCITFSLTAYGQQILGDNFNRPAYDYRSEKNPY
                     YWKNKKPFDGYWQQDVHYKIDAKVDEKTDIISGKVELTYYNNSPDALPFVYFHLYQEA
                     FQPDSYVETLNEVNNVKPTYGKYEAEKKGCDVTEITVTARNGKKVSESTKLTQDITVL
                     KVELNEPVLSGQSITFSIPFNTYFDSGSLRRRMKKFDSGGFEHYDGVHWYPRICVYDR
                     KKGWDTDQHLGKEFYGDYGSYEVTLDFASNYVVEGTGWLENEAEVMPKSLREKLDIRN
                     FAGKPWESPASVVIPYIDGDRKVWKYYALNVHDFAFTADPNYRIGETMAVINGQTVRC
                     IALAQEGHAGFWQNAADYTKQIIETFSADFGPYIWPKIIVADARDGMEYPMLTLDGGY
                     NPAYYDLLIHEVGHMWYFGMVGNNETYRASMDEGFTQFLTGWGYRKISGDYRIDFPEQ
                     NKYVAKYRNPDKIMMSEVYAAYLSDAAREADATLNTHSDMFNSALAHGGGYRHVYFKT
                     AVMLYNLQYVLGDELFLAAMRNYTDDWKLCHPYPEDFRNSISQYTHTDLDWFFDQWLE
                     TTKNIDYGIKKVDKVSSTNVDGQTLLNYEITLDRKGSMQMPIDFSVVLDNGDTLRYYI
                     PNTWFNKYEYQKPSQGRLDRTYFQNVISLPKWYGWDKLNDTYVASITTPHKIKDVIID
                     PSDRLADINKLDNSWKCPTEWVFDSQIRNAPDWDHYTMRWRPEIWGNAYDGLKVGLHL
                     NGDYFAYKHKLSLTAWYNTGLAQGALYDNEFLGNTLDNANEDISDKFDKFNFDFNYRT
                     STDRFLPLSDFILNTKYLDGVFVFETGFEKKLGENNRDLLKVYVKDMQFNNIEYLYGP
                     YVIEGNKNNTVNIDYTHRYNYFTGNGTINVRFRSNDLLSDYDFSRLQFEAINITKLGK
                     LDLHTRYFAQWGYGSNVPFESKLMLGGANIEDMLDSKFTRSRGFFPDEWGEFGTTTNH
                     LQMGGGLNLRGYSGYFAADDIDGETQLAYAGLSGTSLSAELQFDRIVKKQLIRFIKME
                     PYLFADAGIMVGDNAFEEISKPRIDAGIGSAFTWSWWGPLETVKPLTVRIDLPLFLNR
                     PPAEDPEYVKFRYVVGINKTF"
     gene            complement(21675..22319)
                     /gene="rsmG"
                     /locus_tag="PLL28_01935"
     CDS             complement(21675..22319)
                     /gene="rsmG"
                     /locus_tag="PLL28_01935"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNF68102.1"
                     /translation="MAILVSVEHILRYFPELSPLQISQFKQIGAQYKFWNEKVNVISR
                     QDIDNIYQHHVLHSLAIAKFISFEPGTKVLDLGTGGGFPGIPLAIMFPEVEFHLIDSI
                     DKKVKVVESIYKALGLRNVTVDQVRAEDIREKYDFVVTRAVADMMILYDWTINLIRRG
                     HSVNDVPNGLIMLKGGFLDLEIAPFKDILYKFPITEWIPEAYFIEKWLLYLPMD"
     gene            22406..23992
                     /locus_tag="PLL28_01940"
     CDS             22406..23992
                     /locus_tag="PLL28_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNF68103.1"
                     /translation="MAIRMVPDDNQPQQQRKLPGTGGRGGSGGGGGNLITMLLPLLLG
                     IFRKNPKTGCVTLIVIAAVIYFMGGNLGGLLNPGGGGTNITDLFNTGASFDPVKYDAT
                     EIFEPPITDNAKNPLPDAVSLLRYAPTRRNQGSQGSCVGWGSAYAARTILESERTGRN
                     PDDIAFSPAYLYNQIGLEGCQGAYINNAMEVMKDQGLVTLREFPYDDNNCTKQPTSSQ
                     KQQASQYRMNGFNRLTVGDASGTGNEKIDLVAIKQNLAQGAPVVIGMMVGGSFMQSMM
                     GQEVWHPTEQDLSMYGFGGHCMCVIGYDDYKEGGAFQIMNSWGKEWGDNGVAWVPYNV
                     FEHFTVEAYGVYPMGSADKPLGNDLNASFGLVMNDNGRHIPLVKDGENTFSTQSPVKK
                     GTKFKIEITNSKECYTYVFALDENKKSTVLFPYTPKHSPYCGITGTRLFPKDYSMELD
                     AVGSRDEMAIVLSKTPLDYQALNNAANAAAGNYSAKMTQALKGKLQSEVSFSAGDVIG
                     LKTVLAENQAVVMVIGIDKR"
     gene            24040..26316
                     /locus_tag="PLL28_01945"
     CDS             24040..26316
                     /locus_tag="PLL28_01945"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HNF68104.1"
                     /translation="MTTQWRLTAMCLLCTVVMQINAQDTYIPFRTSLPFSLDGALDEP
                     DWQRTPVETAFMQQGPVAGATPTENTEVRIMYDNDYLYVGITCFDSEPDKLIRTELER
                     DFPIGNEDGTGVTIDTYHDKLTGMDFLSNTLDARWDAQVTQDGGGLSESFNTFWDAKT
                     KLYDFGYITEYRIPFSSLRFEEAEQVTMGIRVARLIKRKNELITWPPCDPNTEHVWTN
                     ISFAKPIVFEGLQGGHPFYISPYAIASYLEFQELNAAGTAYEKQAIFMDRKFYVDNEI
                     ADKIISNIGLDVKYGISKNLTLDLTVNTDFAQAEVDDRIINLSKYEVNLPEKRTFFLE
                     SANALSFGFPSGNELFITRRIGNEHGQIVPILAGARITGKANGWLIGALNMQTTGLVT
                     PYDTISPHNFTVLRTRKDIDKLGSFVGGIICNRIDTDTSGKSYQAIGLDIVKRITQQF
                     TIEGGIANTMADLNAENFADGLYSHVGVFKSATSGFTYHSLLDIVGKDVNPVMGYLDE
                     HDYGAWYPQIQYQWDLQKSDLLQYFYISADYGYRWKLSSGDRETTGGSLATGVIFENG
                     ASVEWEPLSIKKDSLFFDWELDAGNAISAGNYLMYNTILDVVLPTQSTYNISLGGTYG
                     GFYGGDRAFAYVNLQYYISSHLNANLTYEFNDIQFDQYLDIDSATSYMSNLVRLGVNY
                     NFSTKVSLKAYIQYEDISDMTSANLRFRYNPQEGTDLYIVLNQGLNNDRDRLDPKLPY
                     AQSQAVTIKFTKTFAVKT"
     gene            complement(26317..28470)
                     /locus_tag="PLL28_01950"
     CDS             complement(26317..28470)
                     /locus_tag="PLL28_01950"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNF68105.1"
                     /translation="MKKLLLILLLILMRISVYADGGMWLPILLEQLNIADMQQRGFQL
                     TAEDIYSVNKSSMKDAVVLFGGGCTGEVISEEGLVLTNHHCGFSSINALSSLEHNYLR
                     DGFWAKNKSEEIPAPGLTVTFIISMHDVTDSIIPYLNPQMDEQTRANVVSQRSAQLSA
                     AFKKGTHYDAMVRSFYYGNAYYLFVTETFRDIRLVGAPPASVGNFGGETDNWVWPRHT
                     GDFSMFRIYAGKDNKPADYNAENVPFKPRYSFPISLKGVEANDFTLVYGFPGRTQEYL
                     TSYAVDLTVNSFNPNRISCRDARLEIMNDYMRGNDTITLQYASKAKGLANAYKKWKGE
                     MLGLQQTDAIGIKQQYETDFQVWANTHVGKDYRYVLDNLKQAYTSFRPYSELADYTSE
                     AFYAVEAINFAGNFRSLVEKSAVDSITNEALGQIAAELNNKSEGFYKNYCLRLDRDMF
                     AVMMEIYSQKVDKQLQSDYFKSQVAAYHGDFKAWANAVYGKSVFTHPDKLNKLLSTYS
                     KKKLKKLSNDPLYKLYIEVTSLYEVQSKQFIQPAQIQIATLMRDYMRGQMAKDNEKIF
                     YPDANSTLRVTYGNVEGYTAKDAVYYNFQTTDRGLLEKYKEGDEEFDLPKPLVDLIKA
                     RNFGAYGDVNGNLPIAFIASNHTTGGNSGSPVINAKGELIGTNYDRVWEGTMSDIMYD
                     VDRCRNISLDIRYTLFIVDKYAGATNIIQELKIVD"
     gene            28623..31763
                     /locus_tag="PLL28_01955"
     CDS             28623..31763
                     /locus_tag="PLL28_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68106.1"
                     /translation="MNRFSFLLATLTTLFITSISAQITPTAKKPWPAMPNSPNYYEVR
                     QAYLDHVANEEMETELLGLEAEDDDVYAKFNRWDYLMKTRVDANGNYPDPRTAFLEWE
                     AYKKSHAEAYEGQRAAGWEPVGNAEVPTDGGGVGRVNVIIQDPIDAGVLYVGTAGGGL
                     WKSPDGGTTWLPLTDNIPVTSIADVVVDYTNNDIIYVVSGDGYGYEASWQADNDFWGG
                     VYSAGILKSTDGGVTWLPTGLSYNQDELMIVQRLVMNPADPNILVAATREGMFRSTDG
                     GDTWTLVSSVHCYDFAQHVTDSDILYAVGDRDVMRSTNAGASWTVLKNNLAGTDDRMS
                     IETSNDNPDKIYVLSYGGQMDISDNGGTTWSSMSSPSGATSFYNYYDLVLDVSNVDEN
                     LIFAGGLEIARSTNGGTSWSKKSVWDDWTASNYVHADSKGLLCDPNDVNIVYATNDGG
                     VFKSTDKGQTWTDISAGLRVAQPYRLSPAYTVTDMVLNGWQDNGCNLWDGTTWKRVQG
                     GDGMDVIIDYSNEDRMYASFQYGYVNRTTNGGISWTPLPVAGGGWLTPYEMDPVDHMI
                     MYYGASTGTIQKSLNGGTSWSNLSSNLGGEVFDIAIAPSNTNYVYACALQTMKVSTNA
                     GTTWTNITSGLPTAGTGFNYIAVSDEDPTHVWVAISGYEDGKKVYFSDNAGSTWTNVS
                     GTLPNVPVNCIVYENVSDDDRVYIGTDIGVFTRTNATDWEPYMTGLPNVMVHELEIDY
                     AEYKIYAATYGRSTWKSDLYDFVAPTLNVGVVDLQYCPGQDVDVPYVGTGTFAAGNVF
                     TAELSNATGSFTTPTAIGTFASTDLAGNIPSVIPTTASGAGYRIRVKSSSPGLTSTDN
                     GANILIDCEQPVDVATDVVTPTTATLDWADVYCAIEYEVRYKSSIDVDYTYVTTTSST
                     YTLTDLNPSTPYEWAVRTICLASPDVSTDFTSSEDFTTGQNAITDLIGVSGIFLYPNP
                     ASDNCALQLNTANAMDMRIELFDVTGQSVVILQNGTIAAGDHSIALDLAHIAAGAYTL
                     KISANGKSAALQLIKE"
     gene            complement(31827..33491)
                     /gene="recN"
                     /locus_tag="PLL28_01960"
     CDS             complement(31827..33491)
                     /gene="recN"
                     /locus_tag="PLL28_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNF68107.1"
                     /translation="MLRSLHISNYAIINEARIGFGEGMHIITGETGAGKSILLGALGL
                     ILGERADSRVLYQKDKKCVVEGTFSIADYGLKSFFTDNNLDYEEETIIRRELSDNGKS
                     RAFVNDTPVNVQVLQQLASKLITIHSQHETLDLGNSAFQLTVIDALAQTGELLTTHKQ
                     AFEAWHLCQQQLEDTISLASRAAQERDFIQFQFEELDKAGLDAIDQEALEAELTQLTH
                     AEEIKRNLMAAVDLMDQGQANIADQLRAAISQLRSVEKYLPTLESYLQRLSSAQIELQ
                     DISRDLDDLASGTYADPSRSEIINQLLSAVYRLQKKHQCADTQALIALRDDLGQKLMV
                     FEQSESRIEALTKETSLLLAKANQSASKLSAKRKAVFASFEKQVKALLAEVGMKDAEI
                     KVSHTFDADRHLTINGADQIQFLFSANKGAALQDIKKTASGGELSRLMLCIKSLLAKS
                     VALPSLIFDEIDTGVSGEVAQKVGNILVRLADNHQVIAITHLPQIASKGAHHLLVYKS
                     NEGGSTHTHIRSLQAEERILEIAKMLSGEKPTKAAIENAKELMLHQ"
     gene            33584..34750
                     /locus_tag="PLL28_01965"
     CDS             33584..34750
                     /locus_tag="PLL28_01965"
                     /inference="COORDINATES: protein motif:HMM:NF022818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing (seleno)protein"
                     /protein_id="HNF68108.1"
                     /translation="MKPWLLVIVIFLLFVVELLRIWFIMPFPGSQEGGTLRLAYFMSN
                     NIWWMRLLLYIITAQFLFRFILRRKVFAAIIVFSMLIIYGVMTYYLVYKLNASAMFKQ
                     QQNPVYATAANNSVNEGKLVIGVVINGEAKAYPIEIIGYHHQVRDVIGRQELMVTYCT
                     VCRSGRVFSPVVNGNITNFSLVGMDKFNAMFEDEETGSWWQQATGIAIAGKMKGTALP
                     EIYSRQMSLHAWLTEFPDSKILQPDDNFLKQYNQLTGYDEGKVNNDLEHRDPLPWQNK
                     SWVIVAKVGEHKRAYDYAELEKRHVINDTLGGKEIAVTLALDNKSFYAFERRNDDVVY
                     QFFYDSKFTNGLGDNNSNLTWSFSGQARLSEDSTIQLRQIPVYQEFYHTFKDFE"
BASE COUNT         9905 a         7091 c         7444 g        10466 t
ORIGIN      
        1 tgcaaagaat atgtttagcg gtaataaaat caataacatc atggcaatag aacctggttc
       61 cataatgcca ttcagcaagt cagcaatttg cgcaatacca ccgtcctgtg atgcatcggc
      121 accaatcaat tttcccgaca tgatgatacc tgctatactt gccatagcgg aaataaatcc
      181 actcaaagta accgtaatca gtttacctaa ataaatttcc agtcgcgatg cagagcttac
      241 taataatgtc tccagggaac cgcgctcttt ctcgcctgca gcaaggtcaa tggccggata
      301 catgcagcct atcatgcaga aaatgatgaa aaaatatggc agtattccgc ctatcatgct
      361 ccctatttgt tcgcgtgaag atgccaggtt gcgggatacc ggttttgcag gttcaagaat
      421 agttcctgat aaatgattat cctccagctt ttccttgatt aattgttgct tatatgcatt
      481 gttcaaattc tcaattatcg caacttcgaa ttcattattc gttgaggtat agaaatattc
      541 gaaagtggta ggttctaatt tcgcgatggc agaatcatat cccgcaggaa aataataggc
      601 aacgtcaatg ctgtcgttgt tgatcatcgt ttgtgccgaa gtggtatctg caatctgctt
      661 tatgctcgta ttcggcatac cggcaacaaa tttgctgaag tgattggcat tgccattgtc
      721 gatgatggct atgcgaagtg tttcctgtcc cgccttttct gtttgaccgg caacatattt
      781 agatatgccg acaaaaatgg caggatatac aaaaagcgga atgatcaaca tcacggtgag
      841 tgtgcgttta tcacgcagga tgtctttgat ctctttctta aatatggtaa atacgttttt
      901 catttgtcat gtagttaagg tgatcaggca gttacatttt cgtgttcttg tgcaatacgg
      961 ataaactcat cctccagagt tgtttcacgc atacccgctt taaaatcagt aaacaagcca
     1021 ttataaataa gatctccctt gtaaataatg gcaagatcat cggcaagcat attgacttca
     1081 ctcatgatat gtgttgaaaa tatcactgtt ttattgtcct ttttgcactg acgaatcaac
     1141 tcaatgatgc tttttgccgc aataacatct aaccctgatg tcggctcatc aaatacaact
     1201 acttgcggat catgaatcat ggttctcgca atggaaactt tctgtttcat accggtagaa
     1261 aattttccta cacgacgatc ggcaaattca tttattccaa gcaaatcgaa caaatatttt
     1321 tttctgtcct gtaacgtctt ttcatccatt ccattcaatc gcccgaaata atctatggtt
     1381 tcatttggag tcaatcgatc atataaaccc gtagatcctg taagaaatcc gagactgcca
     1441 cgcacctgct ctccttgggt aacggtgtcg taattattca cgataatgtt acctgaagtt
     1501 ggcttcagga tggtagctat catgcgcaga gttgttgtct tccccgcgcc attcggacct
     1561 aaaagggtga agatgcgtcc cggttgacaa acaaaagaaa cttcattaac ggcattcaca
     1621 gttttgccac tgtggtccgc tcccagctct ttgcgttgct gacgcgataa tgagaatgat
     1681 ttagaaacct tatctacggt tatcatatgg tttatttttc agtgagttta acaggtgtaa
     1741 cttgatatcc ggcttgttga agcaatttca caactccttc aggtccgcct aaatgtgcag
     1801 caccaactgc tacaaaggta gagccttttt tcgcaaggtc gccaatgatg ggaatccatc
     1861 gtttattccg atctgctacc aacaggttag cataatcatg aaattccggc gttgcagtaa
     1921 tggtaacatt atacagactt tcaatgtcgc cgtttttata ataacttatc atctcatcca
     1981 acctgtcttc agaagtaaat gatgaatcag aaatttcagt catccaatct acctgttcgg
     2041 catatggcac gctatcgaat attttgagtt gcgattcaag tgtttctaaa cctgctattg
     2101 gtcggtgatc ttgcatattg cgttgcatga aatgtaattc atacgactgt gcattttcac
     2161 ccattgattc tgtggtgagc atttggtatg caacaaaggg ttttagttgt atataaggtg
     2221 taaaatctgc ttgcatacta tcgcggaaaa cagatgtcat atatgcaatt tcctcatcac
     2281 tcgcgaaatt atgccaggta acaccttccg gaagcgttgt ccatcgctgg taggttgaaa
     2341 tcatattcgg atcgtcgagc ttgatttcaa atgccagttg tgcggcattg tcgaatgcag
     2401 tttccacttc cggtgctaca taaaagtgct cttgattgat aaggtggaag gtgccaaaca
     2461 aatatgaagt gtctgtcagt ccattaccac tgatggcata gagtattgaa gtactttttg
     2521 ccccttgctg agcattgagt gcaaacggca atagggtaag tagcgatgtt aattttatga
     2581 gtgatttcat tttttctttg attgcatatg tctgctcaaa aatgattcca tctcggcata
     2641 aaaggcgaat ttattttcca tattcatgaa tccatgccct tcattctcgc gaaccatgta
     2701 caatacatct acgccttttt ctgtcaatgc ctgtactatc tgatcgcttt cagatttttt
     2761 tacgcgcgga tctctggctc cctgtgcaac aaacaagggt gctttaatat tgtcgatatg
     2821 cagcagggga gaggcttccg caagtaattc acgatccttt tcaggatgtc caaccatctc
     2881 atacatcatt tccagatacg gtgcccaata tggcggaatc gtttccataa atgaaaataa
     2941 actgctcaca ccaacgtaat ctacaccaca ggtatataaa tcaggtgtaa atgtcaatcc
     3001 tgccagagtg gcatatccac cgtagctggc gccataaatg gcaatgcgat ctttatcggc
     3061 aatacctttt tcgatgagat attgcacacc atcggtaata tcatcctgca tctttttccc
     3121 ccattcctta aacgacgcct ctaaaaatgc acgcccataa cctgtagagc ctctgaaatt
     3181 catttgtaaa acgccatacc ctcggttggc taaaaattgc acttcaggat taaattgcca
     3241 aacatctcta gcccagggtc ctccatgcgg atgtatcacc accggatagg gtgcatgtcc
     3301 ggaatccggt aatgtcaaat aaccatgaat ggtaagccca tcgcgacttt catacgaaat
     3361 tggctccatg ctacaaagtt tatttgcctg caaccatgga ctaacatttg cgagatgggt
     3421 aatagtatta tttgttgcgt gatagagata atagtttccc agcgtgcgat cacttaatgt
     3481 gcgaacaatg aatacttctt cagctttatc acgatcggta atataaattt ctgcatcttc
     3541 atttatcagc gattttactt ttgcatatcg ctgttcactt tcactatcga aaaaaacaaa
     3601 ttctgttttc caggtagtat acgttgccga tgtaatcact tttctttttt tcgaaaaacc
     3661 caactgacct gcatcaacat caggatgttc gaaaagacat tgaatttcat gtgcgtcagt
     3721 gatatcaatt aacacaatag caattttatc tcttccgata ttgctcaagg catacacatg
     3781 tttttgctca aaatcgaaaa acaatggtgc taatgtttcg cgaaatcctg tggtaatgat
     3841 cgttatgaac tgtccgggag ctgtgcgttc cagtaaactt gtgtttacac catcggtagt
     3901 cattgccaaa cgcaatgcac ctgtatggtc agtaatccag gcggtaatat ttcccggatt
     3961 ttcttcaatg agggttatcg cttctgttat agtatttaaa cggtatacat caaatatttc
     4021 aggattgcgc ttattcatgc caataattat agcatcatca acatcttcaa gttcatcaat
     4081 caggtcaact tttacccctt cgaatggcgt aatatctttt gtatcgccgg tatgtacatc
     4141 aacaacaaat atgtgttcgt tttcatcgcc tccggtatcc tgaaaaaagg caagctgatc
     4201 gtcggtaagc cagaaatatg aacggatatc gcgatctgtt cgagctgtaa ggcgcttttc
     4261 gatgttgttt tcgctatcgc ggatgtaaat attcagacgc tgctcataat tcgccaacca
     4321 cgaaatccgt ttcccgccgg gcgataattt gaatccggtt ttttcgggat ttttaaagaa
     4381 gtcttccaat ggtatctgtt cctgctgcat aattaaaggc aaaggtatac gcctgacatt
     4441 gcaaaggtgg tgcaagagcg gttgatagca ctattttttc gaccagtgtt ggtaaaatgt
     4501 cgacgggatg caggcttcta agcgcatgtg gtttaagggt ggtatctttg caccatgaat
     4561 ccgctagtag cacccagtgt tctcagttgc gactttgctt atatcgccag agatatggag
     4621 atgatcaacc gttccgcagc agatttggtg catgtggatg tgatggatgg cgtttatgtg
     4681 ccgaatatca gttttgggtt accggtagtg gctgccatga aacgccatag caccaagcca
     4741 ttagatgtgc acctgatgat ggtaaaccca gagcgttatc tgcaggattt tcgcgatgcc
     4801 ggtgccgata gtatatctgt acacatcgaa gcctgtaccc atattcacat cgccgtacaa
     4861 cagatccaca aaatgggtgc caaagccggt gttgctatca atccgggcac cgccgtaagc
     4921 gctttggaag atatcatcag agacgtggag atcatatgct ttatgagcgt gaacccggga
     4981 tggggtgggc agtcgttcat agagacttcc atagaaaaaa tcggaaggtt gaaggagctg
     5041 atccttaaaa caggctctaa ggctttgata gagattgatg gtggtgtaaa gcttgataat
     5101 gcccagcgca ttttcgatgc cggagcagac atactcgtta gcggcagcgg cgttttcggt
     5161 caacctgatc cggtggctgc catcgcttca ttaaaatcgc tgaccagaca ataactgaat
     5221 gagatcggcg ttagtgcact tgatgtttcg cattcccttg ttgatggcac tcgtatttgc
     5281 cggcaatctt ctgcatgccc aaggaaaagc aacggtgtat ggcaatgtgc gcgatgaaga
     5341 aaataatcct cttccggaag caaatattac cctcctacag ccgaatacta ctattcaaac
     5401 agataaacaa gggaactaca gctttgaagt ccctgcaggc gataccatag tgttggcatt
     5461 cacctttgtt ggcaatgaac ctcactatga aaaggtaata cttgaagagg gtcaacgcta
     5521 tctgttatta gtgaaattaa aatctatcac gctcggaaca gttgttgtca gcgatccgaa
     5581 agaaacaatt ttcaggattc cggttcgtga acttgaacag ataccttcag catccgatcc
     5641 tatcagtgcc atcctcactg cattaggtgc tgtaaagaat aatgagctca catcgcagta
     5701 ttccatccgc ggcggtaatt atgatgagaa cctggtgtac gtaaatgatt ttgaagtgta
     5761 tcgtccatta cttgttcgtt caggtgaaca ggaagggctg ccatttccga attacgacct
     5821 gattgataat atcgctttta gtgccggtgg atttgaagca aagtacggcg ataaactcag
     5881 cagcgttctc gatattcaat ataaagcacc tcgcgaattt gaatccggtc ttactgcaag
     5941 tttattggga gcatcttatt atatcggtga tgccatcagt actgatagag attcattgat
     6001 agattccact aaaggatatt atttatttgg cgcacggtat aaaactaatc gatacctgct
     6061 cagttcatta aatactgaag gtcaatatga accaacattt attgacggac aaggattatt
     6121 tggttggaaa tttaatcgac attcaagtat agaagcactt ggaaatttta gtttgaatag
     6181 ttataccttc cttcccttaa gccgatcaac agcaaccggt gtggtgaatg atgttgtgca
     6241 actcgatgtg tttttcgaag gtcaggaaat agataaattt atcaccggat ttgggggatt
     6301 tagttatcgc tatcaacgag atagttttac tgtttacaag tttttgattt caggatatca
     6361 cagtctggaa acggaaacat ttgatatact gggcgaatat tggctgggtc aggtggaaac
     6421 aaatcttggc tctgaagaat ttggaaatgt attgtatgga ttaggtgtag gggcatttca
     6481 gaatttcgca cgcaattatt tatccagcga tgtatttaat atcagtcatc tgggcacgcg
     6541 cgaatacaac acgcattata tgcaatgggg tgtacgtgca caatacgaat tgattagcga
     6601 tgagttgaag gaatgggaat tgctcgatag tgcaggctat tcgcttccgt ataccggaga
     6661 aacggtgcaa ttacaggaag tatttaattc ctctacaacc ttaaacagtc ttcgttatag
     6721 tggatattgg caagatacct gggaaccgga ttctgcacat atatcactca cggcaggtgt
     6781 gcgaagcagt tattgggatt tgaataatga ggtgactatc actccccgta tccagttcgt
     6841 atggcagcca caatggaaaa acaaactaaa tcagcgacgt aatattgaat taaaagcctc
     6901 taccggatta tattaccaac ctccatttta tcgcgagctc cgcgatctgg aagggaatgt
     6961 gaatacttcc gtaaaaagtc agaagagctt acacgtagtt atcggcagcg attataaatt
     7021 caaagcctgg aatcgcgatt tcaggtttat taccgagatg tattataaat acatgttcga
     7081 tttaattcct tacgatgtag aaaatgtgcg catcagatat tatggtgaga acatggcaac
     7141 aggctacgca gcaggtatcg atctgcgttt gcatggcgaa attgttgaag atgccgacag
     7201 ctgggtgagc ttgagtttca tgcgcacaat ggaagacatt gagggcgaca gtaccgttac
     7261 tgttttagat aaagattcgg atatagattc tttgttatat tctcctcgag gctatattcc
     7321 acgtccaact gatcagcttg tgaatttcgg tatgtttttc caggattata tgccggggaa
     7381 taaaaatttc aaagtgcatc tcagctttct cattggcaca gggttgccgt atggtccgcc
     7441 aaataatgaa tacttcagaa acagtcttcg catcgctcct tatcgcaggg ttgatatcgg
     7501 attttctgcc ttgttgctga gccccaaacg cgatttgatc gggaaaccat ggtacaatac
     7561 tacatttgaa agtttgtggg ccagcgttga agtgttcaac cttcttggca tccaaaatac
     7621 tatcagttat atctgggtga aggatgtaaa taatttgcaa tatgctttcc ctaattacct
     7681 taccgaccgt cgtatcaatg tgaagttggt tgcgaaattt taatatcttc cgttaacttg
     7741 gtgcaataat tactgtttat gatgcaacga ttaatttgcg tgctcttcat attttattgt
     7801 ggacaggcat ttggacaaca agtagatatc aatgcacttc agcctgattc tgtttatgaa
     7861 aatgtatgca gcaagaaaat atattccgat tcattgagca ctacttttgt tatttggatt
     7921 aaacataccg tgccgaaaca ttatcatgca gcacatactg agcaggtgtt tgtgttggag
     7981 ggccatgcaa ccatgttatt gggtgatgtg tggttagatg ttgctcctgg ccagcagata
     8041 tttattccca aaggaacacc acattccgtg aaagtgaagg aaggtgaagt gttgaaggtg
     8101 atcagtattc aatcgccgat gtttgatggc accgatagaa ttaaattgga ataatacaca
     8161 tcaatttttc agtaatacat aagcattcaa cgatgtggca atacaaagcc agataagata
     8221 tggcaatacc aaccaggttg cctgcgaatg attagtgaat ccccaacgaa taatgagcgc
     8281 aacaattatg gtgagcgata aaatcaccac caatcccggc atcaccatat gccagcggaa
     8341 gaatatcggg ttccacatca cattcaatac aaattgaatt gcaaacaaca gatatagtgg
     8401 tcgcaatacc gcagcgctat attttccgga tacgatataa aggaaaatac tgaagcaaag
     8461 cataatggta aaccatgcag cgccaaacac ccatcccggt ggcgtccaag gggctttgtt
     8521 gaggtcatta taccatgtat tgttggggat acttcccatc aacaaacttc cgatacccag
     8581 agcaccgaaa ttaatagcaa gaaatgtgag aaatcgccac attgggtttg tgcgaattgt
     8641 tttatatccg tcacctacaa aacaacaatt acttgatcat gttcaaacct gattcaagta
     8701 attttttcaa tgaagcagat gcgcctgaat tatcagcttc ggcaatagca ttttttaata
     8761 cagacatcag tgcctgctta tcaataccct ctgatttagc aagcgatagg ttcgtgatat
     8821 cctggattgt tcctgccagg caaagtccgc cattgatttt acttttttgt accaaacctt
     8881 gaatggatac tttttttgta ctgttaccgg gtcctttcac tttgtattct gcatgaaagg
     8941 gttgtccctt ttcaagtgtg cgattgagta tcgcctttac ttcttcaaca tcatcatcag
     9001 caataggttt cagataatcg gcaagggtag gttctagctg tccttgttta tatccaagta
     9061 tgcgatacac ttcgtcgctc caatacattt gattattcac cacgtcaagt tcccagttac
     9121 ccatttgcgc gagtttctgt gcttctgtca ggcgttgttc gtttaattta atcgccttgg
     9181 cataatcttc cagttttatt tgcaggcgat ggcgttcaat ggcataagtg atggttcttc
     9241 cgagcaggtt gctatcaaat tgtcccttca caataaaatc ttgtgcaccg gccttcactg
     9301 aattaatggc aactgtgtca tcttccaaac cggtcataac gataacagtt tgtccgggga
     9361 attctctcaa aatagttttt actgtttcaa agccgctgct gtcgggtaat gtaaggtcga
     9421 gcagaacgat atcaaaatcg cgttcttcga gcatgagcat gccttttccc aattcggaaa
     9481 cgcgcgaaat ttgtggttga atcatggccg attcgttcag atagatctct accaaacggg
     9541 catcagccgg attgtcttcg atgaggagga tagtagtttg ctttcggttt tccatgtgtt
     9601 tgaactatgc agatcgacgt ccgggtaatt ttgcaactgt aaaccagaag gtttcaattt
     9661 gtctgatgat atatatgaac tgatccagat ctacgggttt tgtgataaaa cagttggcat
     9721 tcagtttata actcttatag atgtcttgtt cagcatctga ggttgtcaga atgattacag
     9781 ggatgagttt taatacttca tcatttttaa tttctgccag tacttcgcgt ccgtcttttc
     9841 gtgggagatt gaggtcgagg agaataatat cgggaacagg gcttaggctg tatttccctt
     9901 tatgtcttaa aaattcaatt gcttcaacgc catcctttac cacatggagt tcgctcttga
     9961 aaggtccttc tttcatagcc tcctgtgtca ggcggatatc accggggtta tcctccacca
    10021 ggaggatctg catcacgtct tcattgtggg acctcattgt cacggaatta cctctgaatt
    10081 ataaaattcc cccacccccg attacgaaag taggaaataa aatttgttta gacaccctaa
    10141 aaatcaaact atccattcgg cgtattttcc ataaaaacga actgcaggat gttggcaccc
    10201 atttgcagtg ctttctggcg tgtttcttcg gggtctttgt gcacatcgcg atcttcccag
    10261 ccgtcgccaa gatcgcattc gtaggtgtag aggcatacca tccgtccttc ccagaataaa
    10321 gcaaaggcct gaggtggttt gttgtcgtgt tcgtgtattt ttggcagtcc cgtacggaac
    10381 tcataatgtt gatggaagat ggcgtggctg aaaggtaatt ccacaaagga aagctccgga
    10441 aataccttct gcatggcagg tcgcacataa ggatccatac cataattgtc gtcaatatgc
    10501 agaaatccgc ctccaatcag gtaggtacgc aaattttcag cttccgcagc agaaaatacc
    10561 acgttaccat ggcctgtcat atgcacaaac ggataattga acagttcagg actgccaact
    10621 tccacagtcg gtatatcggt ggcgatattc agatttaaat ttttgttgca aaattcagca
    10681 aggttgggta gcgaagtagg gtttgcatac caatcgcccc ctccgttgta ctttaataat
    10741 gcaatagaat aggttggtgc agtcgccggg gcaggtgaaa atgcataacc aagggttacg
    10801 gtgaatagca gtataaataa tatgcgttta ggcatgcagg gttctccagg tttttatggt
    10861 catcacagca ctcatagcag cagtttctgc gcgaagcacc tcgtttccga gacttacttc
    10921 gataaagcca tggtcaatgg ctgcagctac ttctgcctcc gaaaagtcgc cttccgggcc
    10981 gatggcaatg catatcgcat tttctgaaca ggtttttgcc aatggaatca tccctgtttt
    11041 gcggcaatgc gctatccttc gatcggcggt tttgctgacc tgaggtaacc actgcaaaaa
    11101 ttgaaccaca tcgtcaagtt gagggagaaa aggctgtgcc gactgtttaa gcgctgccac
    11161 catatgcgaa agctctttgt cgcgtttaaa gtgcctgcgc tccgtgtttg aggtcagtaa
    11221 tggtgttatc ctgtcgaccc ctaactcggt aagcttttcc atcaagaatg ccatgcggtc
    11281 gctattatgc aataatgcta ccccaatatg cagggaaggc gataaacgct gatgatgggt
    11341 tgcatcagtg atgtggatat gtccttgttt tttattcaac agggtgatat cagcatttcc
    11401 cgttaagccc ttgccattgc tgaaacgaat ttttgttccg atatcggcac gcaatacatg
    11461 caataaatga tgtatttcta catcaggtag ttcggcagta tcgcctgtga aataatcagg
    11521 taaataagca caatgcataa gcaatcagaa aatagagctg ttcaaatcaa aaccaagcat
    11581 cacttcgtgc gatgccacac ccaatgggag atgcatgtcg taaatataac caagggtata
    11641 ccgatcattg aatcttgcat gtatacctgc aagccaactg tgatcattgc catatccgat
    11701 agtggccttc aaatgctcat gccaggtgat ggccgcttgc agatcaagga ttagcggttg
    11761 gccattttcc aatgcggtga atactgctgg tgtgatattc cacgtatcgt tcaggggaat
    11821 gcgataagcg ccatttatga acaatggaat ttccgaagaa gcatcggtat tgttgtcatg
    11881 ttcggcaata ttttcctgca tgaaaccggg agtgccgatg gcaataaaag catcattggt
    11941 gatgtaggca acacctgctc cgatgttaaa tagcgtttta ctgatatcag cctgaagggt
    12001 agggtcgcca ctgcttgcag gatgtgtttt gctgagttca cgcgcgatgt tttttccggt
    12061 gaaggatact ccaccgctta ttgtaccatt ggcaacttta aaatgccggg cataatttag
    12121 tgaaacaata ttttgaatat cgacgccgaa attatcgtgc atgagttgaa tgcctgcttt
    12181 gtttttatca aaggaatatt ctccaataat atttgctgta gttgcattgt cgattgcacc
    12241 gctgaaggta ttgtaataat gagcgtgtat taatgcatct gaatgataag cggtgagtgc
    12301 aggatttatt gaagatgggg tgatggcatg ttcggaaaac acaggcaatt gcccatgtaa
    12361 aaaagcgctt attattgtgc atatgctcaa tgtaaatatt cgcttcatct tgccacagtt
    12421 agtgatccgg tatatgtttt tttattatcc gtttggttca ccacgaacaa ataaatgtaa
    12481 gtgccatcgg gtaaaggccc tttatcaatg ttccaagatg catcccagtt attatcataa
    12541 ccatcggctt cgtatacgat ttttccatat cgattataaa cagtgagatg gttatcaggg
    12601 aaatatgcta aacaactgat ttcccactta tcattgatgc catcgccatt tggagtgatg
    12661 acatcaggaa taacaggatc tgcgcatgcg gtttgggcaa taacggtatt tgttacaacg
    12721 cataatatcg acaccaaaaa caaaccgttc aatttcattt ggctgtggtt ttttcgtcgt
    12781 gaatgattgc tatcggtcta aagccgatga atggtgatgg cccggaaatt cctgcataat
    12841 catcagggcc atctatgcga atatttggtt cagggctatt ccaacctcca cctttgtgct
    12901 ttcctttttc gagcagcatt tcagttgtat ttccactcat gttgtaaagt ccgtatcgat
    12961 tagtggtata cgccatcaca ggagttaatg tgaggctgca tgtatcacct tttttagcat
    13021 tgcaacgata atatccttgt ttgctgatgt aactatcctt cccccagcca taagtattat
    13081 cagcctcgcc accacgcgct gctattttcc attctgtact atcaggtaat cgcacttcaa
    13141 tattaaatcc gcgcagcgaa tatgcttctg tgagccattt gcaatacatc accgcacctt
    13201 cgtaagaaat atttacaaca ggataatgat cataggcttc gtgctgaaaa tagaattttg
    13261 agtaggcact gctatcgcca aaaatagcat cccactgtgc agtatcaact ccggcaatac
    13321 gcaattcatc gtaacgacct tttatttgca agtcattgag gaaatagcga taattttcat
    13381 tgctgatttc atatgcctgc atgtaaaaat catggttcat gccatctgcc tgaccgggta
    13441 tgtacataaa tccgtgacgt ttcaaaaagc ggcctgtctt accatgatga ggaggtgtga
    13501 atcctgtagc caaaacagcc acaacacata atgctgatat ccatgcaatt ttcggcatag
    13561 ggtataaata tactataaaa tatgaacgca catacaacgg ggtatgtgcg ttcgagtcaa
    13621 ttagattcct tgattgcagc tattacttca ccaagcttgt aacagtaact actgtgcgga
    13681 agcctatgaa tggtgaagct tcagttgaat acgggaactc ctgggcggcg tctatgcgca
    13741 aatattcggg ttcgctccac cagttgccgc ctttggtttc tccgtctgtg cttatccatt
    13801 cactaacgtt gccactcatg ttataaagat cgaatccgtt tgggaaatag gattttgaaa
    13861 tggctgtcat gtaagcgccg tcttccgaca atgaagaggt tttataattt gccagataaa
    13921 tacctttttt attacgcaat tcatttcctt cccatgggta aatactacct tgaataccac
    13981 catgagcagc gtatacccat tctgcttttt cgggcaagcg cactttagca cctacgccat
    14041 tggcttttaa ttgttcttcc agccaggtgc aatacaatct tgctgcttgt tgggaaatat
    14101 ttactaccgg ataattgtca aaagcaggat gtgtaaaata gtgctctttc atttcttcta
    14161 atgaagaaaa gggcaatatc catttttctg attgaacgtt ggcgatttca tattcagcag
    14221 tctttccttt agccttgagg tcttcaagaa aggtacggta ttctttattg gtaacttctc
    14281 ctgcctgcat gtaaaatccg tttatggtca gatttatacc atcaacagtt gtggcacctg
    14341 aaggtacgaa aacgaagccc tgttttttca tcacctttgc ctgcttttgt ggggaaaagg
    14401 aagtggttaa tgtaatgagg cagagaatga gtagcgtaga ggtcaagtat ttcatcacaa
    14461 gtttttttat ataacgtttt cgcatagcta agtcgtataa aaaagaaaac ccccgattta
    14521 catcaggggt ttagtattga atatatcata ttaggcagac acaccggctt tcggagcggc
    14581 tgccataatt tcagcatttg ctttatcggc atacttttca aaattcttca cgaattgagc
    14641 ggcgagatta tttgcttttg catcataagc ttgcttatcg ctccaggtat tgcgcggatc
    14701 gagaacttcc gtaggtacgt ttggacaggt ggttgggaaa tgcaatccga aaacatcgtg
    14761 tgttgcataa gaaacgctgt ccagttcgcc attcagtgcg gcagtgatca tggcacgtgt
    14821 ataggataat ttcatacgtt taccggtgcc atatgcgccg cctgtccatc cggtattgat
    14881 caaccagata ttgacattgt gtttgcgcat tttttcaccg agcagttcag catatttagt
    14941 tgggtgcaat ggtaaaaaag gcgctccgaa acatgtgctg aaagtaagct gtggttctgt
    15001 tactcccgct tctgtcccgg ctacttttgc agtgtagccc gacaggaatt gatacatggc
    15061 ctgtcccggt gtcagtttgc tgatcggagg caacactcca aaggcatcgc aggtgaggaa
    15121 gaagatattt tttggtaagc cgcctttact tggcaccatg atattatcga tgaagtggat
    15181 agggtagctt acgcgagtat tttccgtttt ggtaatattg gtaaaatcta cagtacgtgt
    15241 tccgggaatg aattcgacat tctcgagtat agcaccgaat ttgattgcat tgaatatctg
    15301 aggttcattc tcgcgcgtca ggttaacaca tttagcatag caaccgcctt cgaaattaaa
    15361 tacgccttct tcagtccagc catgttcatc atcaccaatc aggcggcgat cagggtcagc
    15421 gctgagtgtt gtttttccgg ttcctgataa accgaagaac acagctacat cgccttcttt
    15481 tccgacgtta gcagagcaat gcatgctcaa tacgcctttt tcctgtggca gccagtagtt
    15541 aaggacggtg aaaatgccct ttttcatctc gccggtatag cctgttccac caatcaggat
    15601 gatgcgtttg gtgaaatcga tcatggcgaa gttcttatcg cgggtattat ccacagccgg
    15661 atctgcctgg aagcccgggg cattgatgat gagccagtcg gggttcatgg ttttgatctc
    15721 ttctgctgtt gggcgcataa acatgttatc gcagaacata tttccccaag ggtattcgtt
    15781 gatcacacgg atattgatgc ggtatttagg gtcggcgcaa gcgtaattgt cgcgcaccca
    15841 aatgtcttta tcgctcaaat aagctaacac cttggcatat agcttatcga aagtggcatt
    15901 atcggtgggt atgttgaagt tattccagtc aacagtatcg gtagttttgg cgtctttaac
    15961 ggtgaatttg tcttttggcg ctcttccgcg gaacttgcct gtatctacaa cgagtgcacc
    16021 actatcggcc attacaccca tgccacgttg aagggtatgc tccaccagtt cggcagggct
    16081 gagattccag aaggcgtgac cggtgtgatt caagcctata gccgcaaggg acgctgcggg
    16141 gttctttatt cctgattcca tctttatact ttaagggttg tttcgggggc aaagatacgg
    16201 aggcaggcag gaaaaaaggg taacatttgt caacgctcac gaaaaagacc gcatgcctct
    16261 taagtgctgt cataaagggt gttgtttgtt tatgattatt ttttgtccat tatgtatcat
    16321 tctgccaatt caaatgcata atttttcttt tccgatactg aacattggaa attatgtact
    16381 ttagcgctag ataaaacaca tgcacaagag tcctcgaatt ctgctttcat ttgctttttt
    16441 tctgatggtt tccggtgttt atgcccaggc attttccaca ctgaaattga cctcgctcca
    16501 caattatgaa atagtggatg atgcgttcaa ccgcagcctg gttggcttat cccctgaaat
    16561 agggtatgaa gcttacaact actcatatca attggaaata gcggcatata taccgcgaca
    16621 gatagattct atggtttatg tatatgacag tttattcggg tatggatttg atgccccagt
    16681 tagtgagacc atgacgattt tcactattgg gcttatgtca aggtactatt tgttttccga
    16741 ttggatgcct gtcaataggt tatatacagt ctcaggtata atgggtgtat ttgaacatgt
    16801 gaaccaagat tttattgcag gtggactgag tgaaggaaaa aaatttacaa ataaaagcct
    16861 ggacctcact tttggcttgg ggtttgaaac gcttatagat gattacctta tgccctttat
    16921 agagacatca gtctatttcc ctgtagcaca aggcgaaaat ccctataata ctgtttatga
    16981 tattactgag ggatacggtg taagaatagc cggcggcgtg aagttgtacc tcagtgatat
    17041 gtaattgata caagataagg tatcaaagat atttttggga tgcaaataag ttagaaacga
    17101 ttttcaatct cttttacatc tgaattgcca ctctttcgag gcgatatctt tttcccggta
    17161 gggcggatat taatccttta aattccatac tcaacaaaac acctgccaga ttacccggtg
    17221 tgagttgcga tttcgattgt atgtcgtcta tatataattc gccgtactcc tgtaaaatgg
    17281 tgtgtatctg ttgttcatcg tcatttaagt catgaaataa ttgttgttgt acatgttgcg
    17341 tttttgtcgt atttgaactc caccccatta tatcaagtac atcttcagtt gattctacca
    17401 tcgccgcttt atttgatttg attaatttat tgcagccggt tgagtatgga tgatgtatct
    17461 gtccagggaa agccattaca tcgcgatgat aatcatctgc aagcagcgct gtgattacgg
    17521 caccgccatt gattcccgtt tctacaacaa taagcccgtc tatcattccg gcaactatcc
    17581 tgtttcttcg cgggaagttt tgacgatccg gattagtatc actcatgaat tcagttagca
    17641 atccaccgtt tgtcatcatt ttatttgctg tttcccgatg cgcaggcgga taaatgcgat
    17701 ctaatcctgt tgccattact cctatggtag gcacattatt ttttacacat gccttatgca
    17761 ttgcaatgtc aatgccatat gccaaaccac tgacaatcaa aatattttca tctttcaatg
    17821 atgctgcaag gtcttcgcaa attccttttc cataacttgt tgcatttcgc gtacccacaa
    17881 ccccaagtat gcgcggggca ttcaaatcgg cattgcctcg actaaataat aaaagtggtg
    17941 catcaggaca ttgttttaat cgccgcggat aaagttcatc gaggtatgtc aaacattgaa
    18001 cctcattttt ttctatgaaa cgcagttctt gttcaacgcg gtcgaaatcg gaaaaccggc
    18061 gaatattatc tgcaacggct tcaccaatgc ccggtacctt caatagatga ctgcgcttga
    18121 ctttaaatac agcttctgca ctgccacaat aactcaccag attccttgcc agcaccggcc
    18181 caattcccgg gatgagggat attgctatta aatgatacat gatttgtaaa tacaagacta
    18241 tatttgtgaa gactataacc gcaaatcaaa agaaatcatg cgccattacc aaaccatttt
    18301 cgttactttt ttttgtataa cattctcgct aaccgcttac ggtcagcaga ttctaggcga
    18361 taacttcaat cggcctgctt atgattatcg cagcgaaaaa aatccatatt attggaaaaa
    18421 taaaaaacct ttcgatgggt actggcaaca ggatgtgcac tacaagattg atgcgaaagt
    18481 ggatgaaaag actgatatta tttcgggaaa ggtcgaatta acgtattata acaattcgcc
    18541 tgatgcactg ccttttgttt atttccattt gtatcaggaa gcctttcagc cggattctta
    18601 tgtcgaaaca cttaatgaag tgaataacgt aaaacctact tatggaaaat atgaggctga
    18661 aaagaaagga tgtgatgtaa ctgaaatcac cgtcaccgca agaaatggaa aaaaggtgag
    18721 cgagtcaact aaactcactc aggacattac tgtgttgaag gttgaattaa atgaaccggt
    18781 tttatcaggt cagtccataa cctttagcat tccatttaat acctatttcg atagcggaag
    18841 cctgcgcaga agaatgaaaa aattcgacag tggtggcttt gaacattatg acggtgtgca
    18901 ttggtatcct cgaatttgtg tgtatgacag aaagaaagga tgggataccg atcaacattt
    18961 gggaaaagaa ttttatggcg actatggcag ctacgaagta acacttgatt ttgccagcaa
    19021 ttatgtggtt gaaggaacag gatggctgga gaatgaagct gaagtgatgc ctaaatcgct
    19081 gcgagagaaa ttggatattc gcaatttcgc aggtaaacca tgggagtcgc ctgcatcggt
    19141 tgttattcct tatatcgatg gcgatagaaa ggtttggaaa tattatgcat tgaatgtgca
    19201 cgattttgct tttacggcag atcctaatta tcgcattggt gaaacaatgg ctgtgattaa
    19261 tggtcaaact gttcgatgta tagcacttgc tcaggaaggt catgcaggct tctggcaaaa
    19321 tgctgcagat tacactaaac aaattattga aactttcagc gctgatttcg gtccttatat
    19381 ctggccgaag attattgttg cggatgcacg cgatggtatg gaatatccaa tgctaacatt
    19441 ggatggtggc tataatcctg catattacga tttgctgata cacgaggttg gacacatgtg
    19501 gtattttggc atggtaggca ataatgaaac ctaccgcgca tctatggatg aaggtttcac
    19561 acaatttctg acaggatggg gatatcggaa aatatcaggg gactatcgca ttgattttcc
    19621 tgagcaaaat aaatacgtgg ctaaatatcg aaatcctgat aaaataatga tgagcgaagt
    19681 ctacgcagcc tatttgtcgg atgctgcacg tgaagctgat gctacactta atacacattc
    19741 cgatatgttt aactcggcgc tagcacacgg tggagggtat agacatgtgt atttcaagac
    19801 tgcggttatg ttatacaatc ttcagtatgt tttgggggat gaattgtttt tggcagcgat
    19861 gagaaattat accgatgact ggaagctttg tcatccgtat ccggaagatt ttagaaattc
    19921 tatttcgcag tatacgcaca ctgatttaga ttggttcttc gatcaatggc tggagacaac
    19981 gaaaaatatt gactatggga ttaagaaggt ggataaagtg tcttcaacca atgtagatgg
    20041 acaaactttg ttgaattatg aaataacctt agatcgcaag ggaagtatgc agatgccgat
    20101 agatttctcc gtggttttgg ataatggtga tacactccgc tactatattc ctaatacctg
    20161 gtttaataaa tacgagtatc aaaaaccctc gcaaggtcgt cttgatagaa cttatttcca
    20221 aaatgtgatc tcattgccta aatggtatgg ctgggataaa ttgaatgata cctacgtagc
    20281 atccatcact actccccata agataaaaga tgttatcata gacccgagtg atcgattagc
    20341 cgatattaat aaattagata attcatggaa atgcccgaca gaatgggttt ttgattctca
    20401 aatcagaaat gctcctgatt gggatcatta cactatgcgc tggcgccctg aaatctgggg
    20461 taatgcctac gacggactaa aagtcggttt gcacttgaat ggcgattact ttgcatataa
    20521 acacaaattg tcgctgacag cctggtataa tacaggtttg gcacaaggtg cattgtatga
    20581 caatgaattt ctaggaaata cacttgacaa tgccaatgaa gatatcagcg ataaatttga
    20641 caaatttaat ttcgatttca actatcgcac gtctacagat agattcctgc ctttatcaga
    20701 tttcattctg aatacgaagt atctcgatgg tgtatttgtt tttgaaaccg gatttgagaa
    20761 aaaactggga gagaataatc gagacctttt aaaagtgtat gtaaaagaca tgcaatttaa
    20821 caatattgaa tatttgtatg gtccttatgt gattgagggt aacaaaaaca atactgtaaa
    20881 tatcgattac acacatcgat ataattactt taccggcaat ggaacaatca atgttcgctt
    20941 ccgcagtaat gatttgttga gtgattatga tttttctcgt ttgcagtttg aagccattaa
    21001 tattacaaaa cttggtaagt tagatttaca caccagatat tttgctcaat ggggctatgg
    21061 cagcaatgtg cctttcgaat ctaaattaat gttaggtggg gcaaatattg aagatatgct
    21121 cgatagtaag tttacgcgat ctcgtggttt cttccctgat gaatggggcg aattcggcac
    21181 tacaaccaat catttgcaaa tgggtggcgg acttaacctg agaggttatt caggctattt
    21241 tgctgccgat gacattgatg gtgaaactca attggcgtat gcaggactaa gcggaacttc
    21301 tttaagtgct gaattgcaat ttgaccgcat cgtgaaaaaa caattgatca gatttattaa
    21361 aatggaacct tacctgtttg ctgatgccgg aatcatggtt ggcgataatg cattcgagga
    21421 aatatccaag ccaagaatag atgcgggtat cggatcagca tttacgtgga gttggtgggg
    21481 accattggaa acggtaaaac cattaacagt gcgcatagat ttaccgctgt tcctgaatcg
    21541 tccacctgct gaagatccgg aatatgtgaa attcagatat gttgttggaa ttaataagac
    21601 attttaattg attcccatca aaataaataa gcccgaacaa ttgattcggg cttatgtatt
    21661 ttatagcaaa gttgttaatc cattggcaga tacaacaacc atttctcaat aaaataagct
    21721 tccggtatcc attccgtgat tgggaatttg taaaggatat ctttgaaagg tgcaatttca
    21781 agatctagaa aaccaccctt gagcatgata agtccattcg gtacatcatt tacagaatgt
    21841 ccacgtcgaa taagattgat agtccaatcg tacaatatca tcatgtcagc caccgcgcgt
    21901 gtaactacaa aatcatattt ttctcggata tcttctgcac gaacctggtc tactgtgaca
    21961 ttgcgcaatc cgagtgcttt gtaaattgat tccaccactt tcactttttt gtcgatagaa
    22021 tcaatcagat ggaattctac ttccggaaac ataatggcta aaggaattcc gggaaaacct
    22081 cctccggtgc cgaggtctaa cacttttgtg ccgggttcaa aagaaatgaa ttttgcaatc
    22141 gcaagggagt gtaaaacatg atgctgataa atattatcaa tatcctgccg agagatcaca
    22201 ttcacctttt cgttccagaa tttatactgg gcaccgattt gtttgaattg gctgatctgt
    22261 aggggactga gttcagggaa atatctgagg atatgctcca cggaaacaag tatagccata
    22321 ttttccgaaa ttttcaaaat aacgatgtta gtgacaggaa tattaccact aaaaaatcta
    22381 ttatcttttc agcaaaattc tagctatggc aattcgaatg gttccagacg ataatcaacc
    22441 ccaacaacag cgaaaactgc ctggaacagg tggcagaggg ggttccggag gtggtggcgg
    22501 caatctgata accatgttgt tgccgctact gctgggtata ttccgcaaaa accctaaaac
    22561 cggatgtgtt accttgatag tgatagccgc agtaatttat ttcatgggcg gcaatcttgg
    22621 aggtctgctt aacccaggag gtggcggcac taatatcact gatctcttca atacgggtgc
    22681 ttccttcgac cccgttaagt atgatgcaac agaaatattt gaaccgccaa ttaccgataa
    22741 tgccaagaat ccattgccgg atgcagtatc tctgcttaga tatgcaccaa cgcgtcgtaa
    22801 tcaaggctca caaggcagtt gcgtagggtg gggaagtgcc tatgctgcgc gtaccatcct
    22861 cgagtcggaa cgaacaggcc gcaaccctga tgatattgcg ttcagccctg cttacctcta
    22921 caaccaaatt ggtctggaag gttgtcaggg agcttacatc aataacgcga tggaggtaat
    22981 gaaggatcag ggattggtaa ccctgcgtga atttccgtac gacgacaata attgcactaa
    23041 acaacctaca tcatcacaaa aacagcaggc ttctcagtat cggatgaatg gcttcaacag
    23101 gcttactgtt ggcgacgcat caggtaccgg taatgaaaaa attgaccttg ttgcaataaa
    23161 acaaaatctg gcacaaggtg cacctgtcgt gatcggtatg atggtcggag gctcttttat
    23221 gcaaagtatg atggggcaag aagtttggca tccaacagaa caggatcttt ccatgtatgg
    23281 atttggaggc cattgtatgt gtgtgatcgg atatgacgac tataaggaag gcggagcatt
    23341 tcaaatcatg aatagctggg gtaaggaatg gggcgataat ggtgttgctt gggtgcctta
    23401 taatgtgttc gaacacttta cagtggaagc gtatggcgtc tatcctatgg gttcagcaga
    23461 taaaccactt ggcaacgatc tcaatgcctc attcggtttg gtgatgaatg ataatggtcg
    23521 acatattcct ctggtaaagg atggcgaaaa tacattctct acgcaatcgc ccgttaagaa
    23581 aggaacgaaa tttaaaatcg aaataacgaa ttccaaagaa tgctatactt acgtatttgc
    23641 tttggatgag aataaaaaaa gcaccgtact tttcccatac acgccaaagc attcccctta
    23701 ttgcggtatc accggaacca gactttttcc gaaggattac agtatggaat tagatgcagt
    23761 tggcagtcgc gatgaaatgg caatagtatt gtcaaaaaca ccattggatt atcaagccct
    23821 aaataatgct gcaaatgcgg ctgccggaaa ttattccgca aaaatgacac aggcattaaa
    23881 aggaaaattg caatcagagg tgagcttttc agccggtgat gtcattggct taaaaactgt
    23941 gcttgctgag aatcaagcgg tggtgatggt gattggtatc gacaagcgat aaacatatcc
    24001 tgaaatcaca tcatggatta tattttttaa ttttattcca tgacaaccca atggcgccta
    24061 acagcgatgt gcttgctgtg tactgtggtg atgcaaataa atgcccagga cacctatatt
    24121 ccttttagaa cttcattgcc tttttctttg gatggcgcac ttgacgaacc cgactggcag
    24181 cgcactccgg ttgaaacggc tttcatgcaa caaggtccgg ttgccggtgc tacaccaaca
    24241 gaaaatactg aggtacggat catgtatgat aatgattatt tgtacgttgg cattacctgt
    24301 ttcgactcag agcccgataa attaattcgc acagaacttg aacgtgattt cccaatcgga
    24361 aatgaagacg gcacaggcgt aactatagat acttatcatg ataaattaac aggcatggat
    24421 tttctttcca ataccctcga tgccagatgg gatgcacagg ttacacaaga tggtggcggt
    24481 ttgtcagaat cgtttaatac tttctgggat gctaaaacta aattatatga ctttggatat
    24541 atcacagaat atcgcattcc cttttcgtca cttcggtttg aagaggcaga acaggttaca
    24601 atgggtattc gcgttgccag attaatcaaa agaaaaaatg aactcatcac ctggcctcca
    24661 tgtgacccga atacagaaca tgtatggacg aatatttctt ttgccaagcc aatcgttttt
    24721 gaaggtttgc agggcggaca tccattttat atcagtccct atgctatcgc atcttatctt
    24781 gaatttcagg agttaaatgc agcaggaaca gcctatgaaa aacaagcgat ttttatggat
    24841 cgcaaatttt atgtcgataa tgaaattgca gataaaatta tcagcaatat cggattggat
    24901 gtaaaatacg gcatcagtaa aaatctcacg ctggatctaa ctgtaaatac agattttgct
    24961 caggcagaag ttgatgatcg cattatcaat ttatcgaaat atgaagtaaa ccttcctgaa
    25021 aaaagaacat ttttccttga atccgcaaat gcactcagtt ttggatttcc ttcggggaat
    25081 gaattattta ttacgcgaag aattggtaac gaacatggac aaatagtgcc aattttggca
    25141 ggtgcacgca ttacaggaaa agcaaatggt tggttgattg gtgcattgaa tatgcaaaca
    25201 acaggactag taacgccgta cgatactatt tcaccacaca atttcactgt attgcgaacc
    25261 agaaaagata tagacaagct aggaagcttt gttggcggta ttatttgtaa tagaattgat
    25321 acggatactt caggaaaatc ttatcaggca ataggtttgg atatagtaaa acgcataaca
    25381 cagcaattta ccattgaagg cggtattgca aatactatgg ctgatctgaa tgctgaaaat
    25441 tttgctgatg gattgtattc ccatgtcggc gtttttaaat ccgcaacttc aggatttacc
    25501 tatcattcat tgctcgatat tgtcggcaaa gatgttaacc ctgtaatggg ctacctcgat
    25561 gaacatgatt acggagcttg gtatccgcaa atacaatatc aatgggactt gcaaaaaagt
    25621 gatctcttgc aatattttta tatttctgcc gattatggat atcgatggaa attatcatca
    25681 ggcgacaggg aaactacggg aggatcactt gcaacaggtg tgatatttga aaatggcgct
    25741 tctgttgagt gggaaccgct ttctataaaa aaggattcac ttttttttga ttgggaactg
    25801 gatgccggaa atgccattag tgccggaaat tatttaatgt ataataccat actcgatgta
    25861 gtgctcccta cgcaatcaac atataatatt agtctgggtg gtacctatgg tggattttat
    25921 ggtggagata gagcatttgc ttacgttaac ctgcaatatt atatcagcag tcatctgaat
    25981 gcgaatctta cttatgaatt taatgacatt caattcgacc aataccttga tattgatagt
    26041 gcaacttcat atatgagtaa cctcgttcga ttaggtgtta actacaactt ctcgacaaaa
    26101 gtttcattga aagcctatat tcaatacgaa gacatctcag acatgaccag cgccaatttg
    26161 cgattcaggt ataatcctca ggaaggaaca gatctctata ttgtattgaa tcaaggactc
    26221 aataacgata gagatcgtct tgatcctaaa ttaccttacg cgcaatcgca ggctgttacc
    26281 attaaattca ccaagacctt tgccgtaaaa acctgattaa tctacaattt ttaattcctg
    26341 aataatattc gttgcgccgg catatttatc gacgatgaat aaggtatatc ttatgtcgag
    26401 tgaaatattg cgacagcggt ccacatcata cataatatcg ctcatagtgc cttcccacac
    26461 gcgatcgtaa ttggtgccaa tcagttcacc tttcgcattg ataacagggc ttccgctatt
    26521 gccgcctgtt gtatgattac tggcgataaa tgctatcggc aaattgccat tcacatcgcc
    26581 gtaggcacca aaattgcgcg ccttgatgag gtcaaccagt ggttttggta gatcgaattc
    26641 ttcatcgcct tctttatatt tttccagtag accacgatca gtagtttgaa aattatagta
    26701 aacggcatct ttagctgtat aaccttcaac atttccatag gtaacacgta atgtcgaatt
    26761 ggcatccgga taaaatatct tttcattgtc cttggccatc tgcccccgca tgtaatcgcg
    26821 cattaaagtg gcgatttgaa tttgagcagg ttgtatgaac tgtttggatt gcacttcata
    26881 taatgaagtt acctcaatat acaatttata taaagggtca ttacttaatt tctttaattt
    26941 ctttttagaa taggttgata acagcttatt caatttatca ggatgtgtga atacactctt
    27001 cccataaaca gcattcgccc aagccttgaa gtctccatga tatgctgcaa cttgcgattt
    27061 gaaataatca ctttgtaatt gtttatccac cttctgcgaa taaatttcca tcattacagc
    27121 aaacatatcg cggtcgagac gtaagcaata atttttataa aacccttcgc tcttattgtt
    27181 tagttcggca gctatttgcc ccagtgcttc attagtaata ctgtcgacag ctgatttctc
    27241 aaccagactt ctgaaattcc ctgcaaaatt gatggcttcc acagcataga atgcttcact
    27301 ggtgtaatcg gctagctcag aataaggtcg aaaggatgta taagcctgtt tcaaattgtc
    27361 gagtacatac cgataatctt ttccaacatg cgtatttgcc caaacctgaa aatcagtttc
    27421 atattgttgt ttgataccaa ttgcatctgt ttgctgcaat cccaacatct cgcctttcca
    27481 tttcttgtat gcattggcga ggccctttgc ttttgacgca tattgtaggg tgatggtatc
    27541 atttccgcgc atgtaatcat tcataatttc gagtcgtgcg tcgcgacatg aaatgcgatt
    27601 tggattgaag ctatttacag taaggtcaac tgcgtatgaa gttaaatatt cttgggtgcg
    27661 tcccggaaaa ccatacacta atgtaaaatc atttgcttcc acacctttta aagaaatagg
    27721 aaatgaatat cgaggtttga atggcacatt ttctgcattg taatctgcag gcttgttgtc
    27781 cttacccgca taaatgcgaa acatgctgaa gtcgccggta tgtcttggcc acacccaatt
    27841 atctgtttcg ccgccaaaat tcccgacaga tgccggaggt gctcctacaa gtctgatgtc
    27901 gcggaatgtt tccgttacaa ataaataata tgcattgcca taataaaacg aacgcaccat
    27961 agcatcataa tgtgttcctt ttttaaatgc agcactcagc tgtgcactgc gttgactaac
    28021 cacattagca cgagtctgtt catccatttg cggatttaaa tatgggatga tgctatcagt
    28081 tacatcatgc atgctgatga taaaagtaac tgttagcccg ggagctggga tctcctcact
    28141 tttatttttt gcccaaaatc catctctcaa ataattatgt tcaagagaac tcaaggcatt
    28201 aatacttgaa aaaccacagt ggtggttggt gagcacaagt ccttcctcag agatgacttc
    28261 tcccgtacat cctcctccga atagaacaac tgcatccttc atactgcttt tattcaccga
    28321 ataaatgtct tcagcggtta actgaaatcc acgttgttgc atgtccgcaa tattcaactg
    28381 ctccaacaat atcggtaacc acatgcctcc atcggcatac accgatatac gcatcaaaat
    28441 caataagagg ataagcagta atttcttcat aaaatgcatt atttgtgaca acaaaattac
    28501 gtgatttcgt gtttgtgttt gtgaatgact tgttaatact agttcgagga attgtcaaat
    28561 tgttgtaatc cagaaaaaaa ttaagccgaa aattttgtaa ttttaacaca taatgtaatt
    28621 gcatgaaccg attttcattc ctcctcgcaa ccctgaccac actgtttatt acgtctattt
    28681 cggcgcaaat tacgcctacg gcaaaaaagc catggcctgc aatgcctaat tcacctaatt
    28741 actatgaagt tcgtcaagcc tatctcgatc atgttgccaa tgaagagatg gagactgaat
    28801 tattaggttt agaggctgaa gacgatgatg tatatgcaaa atttaatcgc tgggactacc
    28861 tgatgaaaac aagggtagat gccaacggaa actaccccga tcccagaacg gcattcctgg
    28921 aatgggaagc atacaagaag agccatgctg aggcctatga aggtcagcgt gctgccggat
    28981 gggagcccgt tggaaatgct gaagtgccga ccgatggtgg tggtgttgga cgtgtgaacg
    29041 tgattatcca agatcctatt gatgccggcg ttctatatgt aggaactgca ggaggtggat
    29101 tatggaaatc gcctgatggg ggcactacat ggctgccatt gacagataat atccccgtta
    29161 cctctattgc tgatgtagtt gttgattata ctaataatga tatcatatat gtagtttcag
    29221 gcgatggcta tggctatgaa gcttcttggc aggccgacaa cgatttttgg ggcggtgtat
    29281 atagcgcagg aattttgaaa agcactgatg gcggtgtaac atggttgcca acaggattga
    29341 gctataacca agatgaattg atgattgtgc aacgtctggt gatgaatcct gctgatccta
    29401 acattttagt agcggcgact cgcgaaggga tgtttcggtc aaccgatggc ggagatacct
    29461 ggaccttggt aagcagtgtt cattgctatg attttgcgca gcatgttaca gatagtgata
    29521 ttttatatgc agttggcgat cgcgatgtaa tgcggtctac caatgcaggc gcatcatgga
    29581 cggtattgaa aaataatctt gcaggtactg atgatcgcat gagcattgaa acatctaacg
    29641 acaatcctga taaaatatat gtgctatctt atggtggtca aatggatatc tccgataatg
    29701 gcggtacaac ctggtcatct atgagttctc cttcaggagc tacatctttc tataattatt
    29761 atgatcttgt tctcgatgtt tcgaatgtag atgaaaatct cattttcgca ggcggattag
    29821 aaattgccag gtcgacaaac ggaggtacaa gctggtcgaa aaaatctgta tgggacgatt
    29881 ggacagcatc aaattatgta catgcagatt ccaaaggatt actctgtgat cctaatgatg
    29941 taaatatcgt ttatgccacg aatgatggag gtgtatttaa atctaccgat aaaggtcaaa
    30001 cctggactga tatcagcgca ggtctccgtg tagctcaacc ttatcgttta tctcctgctt
    30061 ataccgtaac agacatggtg ctcaacggat ggcaggataa tggttgcaat ttatgggacg
    30121 gtacaacatg gaaacgcgtt caaggaggcg atggaatgga tgtaatcatc gattattcca
    30181 atgaagatcg tatgtatgcc tctttccaat atggatatgt aaatcgtaca acgaacggtg
    30241 gaatttcctg gactccactt ccggttgcag gtggtggttg gttgactcct tatgaaatgg
    30301 atcctgttga tcacatgatt atgtattatg gtgcttctac cggaactatt cagaaatcat
    30361 taaatggtgg aacctcatgg tcaaaccttt cttcaaatct tggtggtgaa gtatttgata
    30421 ttgccatcgc accctcaaat acgaattatg tttatgcttg tgcattgcaa accatgaagg
    30481 tgtctaccaa cgcaggaact acctggacaa atatcacatc aggactacca actgcaggta
    30541 cagggtttaa ttatattgct gttagtgatg aagatccaac acatgtttgg gttgctatca
    30601 gcggatatga agatggcaag aaagtatatt tttctgataa tgccggttca acatggacaa
    30661 atgtctccgg aacgctgcca aatgtccctg ttaattgcat tgtttatgaa aatgtcagtg
    30721 atgatgatcg cgtgtatatc ggaacagata ttggcgtgtt tacccgcaca aatgctactg
    30781 actgggaacc atacatgacc ggcttaccca atgtgatggt gcacgaattg gaaatagatt
    30841 atgccgagta taaaatttat gcagcaactt atggtcgcag tacatggaag tcagatttgt
    30901 atgattttgt tgctccgaca ctcaatgtag gtgttgtaga cctgcaatat tgcccgggtc
    30961 aggatgttga tgtaccatat gtaggaaccg gaactttcgc agccggcaat gtatttacag
    31021 cggaattaag taacgctaca ggtagtttca ctacaccaac tgcaattggc acttttgcct
    31081 caactgatct tgccggaaat attccatctg ttattccaac tactgccagt ggcgcaggct
    31141 accgtattcg agtgaaatca agttctcctg gccttaccag caccgacaat ggcgcaaata
    31201 ttttaataga ttgcgagcaa ccggttgatg ttgctactga tgtggttaca ccaacaacag
    31261 caacactcga ttgggcagat gtatattgcg caattgagta tgaagtgcga tataaatcat
    31321 caatagatgt tgattatact tatgtaacaa caacatcttc gacttacacg cttaccgacc
    31381 ttaatccaag cacaccatac gagtgggctg tgcgtaccat atgtcttgct tcaccggatg
    31441 tgtctaccga tttcacaagt agcgaagatt ttactacagg acaaaatgcc atcactgatc
    31501 tcatcggagt aagtggcatc ttcctttatc cgaatccggc aagtgataat tgcgcattac
    31561 aattaaatac tgcaaacgcc atggatatgc gcatcgaatt atttgatgtt accggtcaat
    31621 ctgtagtgat attgcaaaat ggaactattg ctgccggaga tcattcaatt gcgttagatc
    31681 ttgctcatat tgcagcaggt gcatatacgc tcaaaatctc tgctaacgga aaatcagcag
    31741 cattgcaatt gataaaggaa taaatttaaa ctgaaattat ttcaaaagga cttgctttat
    31801 tgcaggtcct tttttattga ttgaattcat tgatgaagca tgagctcttt ggcattttca
    31861 atggccgcct ttgtaggctt ctcgccactc agcatttttg caatttccag tatgcgttcc
    31921 tcagcctgta agctcctgat atgtgtatgt gtactgccac cttcattaga tttatacacc
    31981 aataaatgat gtgcaccttt tgaagctatt tgcggcaaat gtgtgatagc aattacctgg
    32041 tgattatcag ctagtcgcac aagaatatta ccaacctttt gcgcaacctc cccggatact
    32101 ccggtatcaa tctcatcgaa tattagtgaa ggtaatgcta cactttttgc caggagcgat
    32161 ttaatgcata gcatcaaacg agataattcc cctccactgg cggttttttt gatatcctga
    32221 agtgcggctc ctttattggc tgaaaataaa aattgaattt ggtctgcacc attaatggta
    32281 agatgacgat ctgcatcaaa tgtatgcgaa actttgattt ctgcatcttt catccctact
    32341 tccgcaagca acgccttcac ctgtttttcg aaagatgcaa aaacagcttt gcgttttgct
    32401 gataatttgg acgcgctttg attcgctttg gcgagcagta aactagtttc tttggtcaac
    32461 gcttcaatac gcgattcact ctgttcaaaa accatcagct tctgacctaa gtcatcacgc
    32521 aatgcaatta aagcttgtgt gtcggcacat tgatgcttct tttgcagtct gtaaaccgca
    32581 cttaacagct gattgataat ttcacttctc gatggatctg cataagttcc tgaagcaaga
    32641 tcatcaaggt cgcgggaaat atcctgtaat tcaatctgtg cagatgaaag tcgttgtaag
    32701 tacgattcta atgttggcag atatttttct acactgcgca actgacttat cgcagcacgc
    32761 agttgatcag caatattagc ttgcccttga tccattaaat caacagccgc catcaaattg
    32821 cgcttgatct cctcagcatg tgtcaattgc gttaattcgg cctccaacgc ctcctgatca
    32881 atggcatcta gtccggcctt atcaagctcc tcaaactgaa actgaataaa atcgcgttcc
    32941 tgtgcagcgc gtgatgccag tgaaatggta tcttccaact gctgctgaca caagtgccat
    33001 gcttcaaatg cttgtttatg cgtagtaagt aattctcctg tttgtgccaa tgcatcaatc
    33061 acagttaact gaaaggcaga attccctaaa tcaagtgttt catgttgcga atgaatggtg
    33121 attaacttcg aagctaattg ttgtaatacc tgcacattta caggagtatc gtttacaaac
    33181 gctcttgatt tcccgttgtc acttaattct ctgcgaataa tggtttcctc ctcataatcc
    33241 agattattat ctgtgaagaa tgatttcaat ccataatctg caattgaaaa agtgccttca
    33301 acaacacatt ttttatcttt ctgatataac actctgctgt ctgcacgttc gcctaatatc
    33361 aatcccaaag cccccaacaa aattgattta ccggcaccgg tttctcctgt aataatatgc
    33421 atgccttcgc caaacccgat acgagcttcg ttgataatgg catagtttga tatgtgcaga
    33481 ctgcgcaaca tggttcaaag ataatgcaaa ctgcatgcac atgagcgttt gcctttacag
    33541 gagttttaca ctccattttc acatgccttt ctatctttgt agcatgaagc catggctgct
    33601 cgttattgtc attttcctct tatttgtggt tgaattactc cgtatctggt ttatcatgcc
    33661 attccccgga agccaggagg gtggcacgct tagactcgca tatttcatga gcaataatat
    33721 atggtggatg cgattgctcc tgtatatcat cacagcgcag tttctatttc gttttatttt
    33781 gagaaggaaa gtatttgctg caatcatagt attttctatg ctgataatat atggcgtgat
    33841 gacctattac ctggtatata aattaaatgc ctccgccatg ttcaaacagc agcaaaatcc
    33901 tgtttatgca actgctgcaa ataatagtgt gaatgaagga aaactcgtaa taggcgtagt
    33961 aatcaatggc gaagcaaaag catatccaat cgaaattatc ggttatcatc atcaggtgcg
    34021 cgatgtcatc gggcgacaag aattgatggt aacttattgc actgtatgca gaagcggtcg
    34081 tgtttttagt cctgttgtga atggtaacat cactaatttt tctttagtgg gcatggataa
    34141 attcaatgcc atgtttgaag atgaagaaac cggttcatgg tggcaacaag ctaccggtat
    34201 tgctattgca ggtaagatga agggaacagc attaccggaa atttacagcc gacaaatgtc
    34261 attacacgcc tggctgacag aatttcctga ttcaaaaatt cttcaacctg atgataattt
    34321 tctaaagcaa tacaaccaac tcacaggtta tgatgaagga aaagtaaaca atgacctcga
    34381 acatcgcgat cccttgccat ggcaaaataa atcatgggtc atcgtagcaa aagttggtga
    34441 gcataaacgt gcttatgatt atgctgaact tgagaaaaga catgtgatca acgatacctt
    34501 gggcggaaaa gaaattgcgg taacgctcgc actcgataat aaatcattct acgcctttga
    34561 acgtagaaat gatgacgtag tttatcaatt tttctacgat agtaaattta caaacggtct
    34621 tggcgataat aattccaatc tcacatggtc tttttccgga caagcaagat tgagtgaaga
    34681 ctcaacaatt caactcagac aaattccagt ttaccaggaa ttttaccata ccttcaagga
    34741 ctttgaatga gaggagagag gagaaaggaa agaggagcaa ggagagagtg ggtgagtggg
    34801 tgagtgggtc agtgggtgag tgggtgagct tgcctcgcct ccggcgggtg gtgagctcag
    34861 ttcgctcgct tttgcgagcg aataattgca tttgtctcac tactca
//
LOCUS       DAOQHY010000012        33626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_537_length_33626_cov_5.470823, whole genome shotgun sequence.
ACCESSION   DAOQHY010000012 DAOQHY010000000
VERSION     DAOQHY010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 33626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33626
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_537_length_33626_cov_5.470823"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PLL28_01970"
     CDS             <1..581
                     /locus_tag="PLL28_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNF68109.1"
                     /translation="VADKPTFAQVADELNAYLDNCDLAGYNSNKFDVPMLVEEFLRCG
                     KGFDENRRYIDVMRIFTLMEKRTLEAAYRFFCNKTLENAHSAEADVTATYEVLLGQLD
                     KYGDALAHDVPSLHEFTKDGDFVDFGRRMIMKNGVPCFNFGKYNGQSCEDVYRREPQY
                     FDWIYKSDFPEHTKLKLRLIALKIKNDKNKAS"
     gene            586..1314
                     /locus_tag="PLL28_01975"
     CDS             586..1314
                     /locus_tag="PLL28_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNF68110.1"
                     /translation="MARALVIIPTYNEQANIADMITAVLSLPQDFDLLIIDDNSPDGT
                     ASAVTAMQSTYPTRLHLHQRAGKLGLGTAYIAGFKWGLAQGYDYMIEMDADFSHKPED
                     LPRLLDACKNGADVAIGSRYIKGGNVEDWPTDRILLSKWASRYVRMITWIPVHDTTAG
                     FVCYKRNVLDTIDLDKIRFIGYAFQIELKYAAWRSGFSLKEIPIVFKDRQKGISKMSK
                     GIFKEAVYGVITMRWKSFFHSYTK"
     gene            1321..2403
                     /gene="corA"
                     /locus_tag="PLL28_01980"
     CDS             1321..2403
                     /gene="corA"
                     /locus_tag="PLL28_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNF68111.1"
                     /translation="MSSEQRHKRKGIHRKKSGLPPGTPIYTGAKKHEIITLQRFAYDT
                     DTIEEINCKAGDKIDLIPREGKVLWVNINGLHQEALIEEIAATFKLHNLVLEDILNVF
                     QIPKAEEYPDTDQIYIALNEFYFAAEGKLERDQISIILGNDYVLSFQEEEGDYFELIR
                     DRLRNAKGKLRKKSGDYLVYVLIDSVVDSYYVVLDHYATELDSIENDIFEKKNRNHLE
                     RIHQLNKDLIYLRRSIAPLKEVLFKLLKDDVILINPESKLFLRDVLDHVNQVVNQIDV
                     DREYLADLVQTDMANMNAHLNEIIKVLTLISTIFIPLTFIVGVYGMNFGNIPLMQTTY
                     GYFVVWGVMIFITLLQFFIFKRKKWL"
     gene            2434..3231
                     /locus_tag="PLL28_01985"
     CDS             2434..3231
                     /locus_tag="PLL28_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68112.1"
                     /translation="MRLTLPIFISACILTSNLQAQSTIAPPANTSVLFNINFDLGIPM
                     NEFKTYNDEVAAGGGFSLFFQPSNKIPVLVGFDMAFMGNGHKTQHETLTADIVAGTTV
                     IETLYFPMRVETYNMITTGDVHLRVQSPTNFFKPYLDGMVGFNHFGTNTSVYDESEEY
                     YLSEEDNPLITSSQQNSSWSLNYGGALGMLVQLNENIFLDVRCAYTFGGEAEYYVEDD
                     IENWEIEFNTVPTSQDDVSDEDIDISAIKKQSHTDMIMGTIGLTFKF"
     gene            3358..3987
                     /locus_tag="PLL28_01990"
     CDS             3358..3987
                     /locus_tag="PLL28_01990"
                     /inference="COORDINATES: protein motif:HMM:NF017102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HNF68113.1"
                     /translation="MRSGRRKLSHKVHLVYGLVVIIAVGIAFFILHNLKASNASQLND
                     SSATPVIDSLHHIPVYYNGDVDHVAGRSVARNGYNYGLKWQCVEFVKRYYADHLGHYM
                     PDADGNAKDLFDKRLPDASFNEARALVQYANPSLKKPQPDDIIVFDGHLGNRYGHVAI
                     ISQVTETSIEIIQQNAGPDTPTREWLSLEKVDNRWEVDHKHVLGWLRVE"
     gene            complement(4056..4565)
                     /locus_tag="PLL28_01995"
     CDS             complement(4056..4565)
                     /locus_tag="PLL28_01995"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HNF68114.1"
                     /translation="MKKLITLVFCAAVGFNTAHAASLPEPEDESTKLNLWIPGVVMRI
                     AADIVEDHMDAEGAATGALLRKFGSINICIREGEAYADRTDKKITRKLDRMERRDYEQ
                     LMSVRTDEETVNISIRSNKHDKIKRMVLLVDEKEETFVYLKMHCRLKVEDIPQLVQSM
                     NFDDDDSDK"
     gene            complement(4590..5111)
                     /locus_tag="PLL28_02000"
     CDS             complement(4590..5111)
                     /locus_tag="PLL28_02000"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HNF68115.1"
                     /translation="MKKMLFTACALMISAMAFAQSAQIDQFFKKYEGKEGFTSVIVTE
                     KLFSLVASATTDDADLKNMIDGIKGIRILVYENAEGASNSGAYYNEFMTTVGTNGYEE
                     LMNVNAEGEKVRLYGKNITGSTLNDMLMVCDADGEFVLISILGAIDLSKISQISDIDI
                     EGLDELKKVEETK"
     gene            complement(5226..5699)
                     /locus_tag="PLL28_02005"
     CDS             complement(5226..5699)
                     /locus_tag="PLL28_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68116.1"
                     /translation="MADYLQTGITTVKELFQFLADRSTSRDRIKNALLRELRDNMKLL
                     EHRDKSGVNVMAMIDGLSASAIAEAYSANYKFDTLCEGPKKLPAAVILSKQQEKYIGW
                     NVERFMYAIEGKIRDLKNLPKLYSNLETAPVNLSVRLNNLYNQMLLLVIFISRKA"
     gene            complement(5724..6200)
                     /locus_tag="PLL28_02010"
     CDS             complement(5724..6200)
                     /locus_tag="PLL28_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNF68117.1"
                     /translation="MHAQLTPWWQEQQVDSTEIKELAKADSIIAYAKTFIGTPYVWGG
                     TDPQHGFDCSGFICYVYKAFNITLPRTSDMQFDAGIPIPYVDARPGDIIVFSSATVVP
                     GDPGHVGIVLDYDAENGFSFIHTSSPETGGVRISNEATEKYYNKHFLEIRRVITAN"
     gene            complement(6257..6727)
                     /locus_tag="PLL28_02015"
     CDS             complement(6257..6727)
                     /locus_tag="PLL28_02015"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HNF68118.1"
                     /translation="MSKKEKKEEVVVRKLTPVLPDHLFVARTPDKGRAVFTSKAVKAG
                     DTLEVAPMIVFSEKDRLSIDDTFLYEYYFEWGKKGKKGALALGFGSLYNHSYSPNARY
                     IPDFDLNVLEFQAVRDIEPGEEITVNYNLDPEDQTPVWWERDAKKKKKKKSQHP"
     gene            complement(6735..6986)
                     /locus_tag="PLL28_02020"
     CDS             complement(6735..6986)
                     /locus_tag="PLL28_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68119.1"
                     /translation="MSKRNAKKRIGAAVASMIDRVLFMDITDEQLESKINALFNTYEA
                     AVKKINGSSKVKGKSAVKKHFTALFADVKKQLEDVVSAK"
     gene            7046..7357
                     /locus_tag="PLL28_02025"
     CDS             7046..7357
                     /locus_tag="PLL28_02025"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNF68120.1"
                     /translation="MFDIKKAMEMKKQMEEIQKRLDNITVEGVSGDGKFQVVITITAN
                     KKVKSVHISDELMASGDKEHLEDLLVIAMERGLDKATQVSEAEARNVALSGGLNGLFG
                     S"
     gene            complement(7358..8416)
                     /locus_tag="PLL28_02030"
     CDS             complement(7358..8416)
                     /locus_tag="PLL28_02030"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNF68121.1"
                     /translation="MFKGLRIIELAGVMAGPAAGSFFSELGADVVKIENPRTGGDITR
                     QWKLPSENPAASTSAYFAAVNWRKLHLFLDLEDPSCLEQVYDMIRQADVMICNWKKGD
                     AQKFKLDPNTVAALNPGIIYANINGFGDEEDRVAFDVVLQAETGWMFMNGTPSSPPVK
                     LPVAIIDLFASHQLKEGILTALIQKYKTGKGSRVSVSLFDASIAALANQAANYITAGH
                     IPQRNGSLHPNIAPYGEILTCKDGVQVVLAIGSDRQFKNLCDILNCPEVYADQRYAHN
                     TDRVHNRNTLIGVLQSKVQSYSSDSFLELCRQNHVPVGAIRDLQQVFELPSAKELILS
                     DDAGKRVKSAIFKVEELV"
     gene            8651..9886
                     /locus_tag="PLL28_02035"
     CDS             8651..9886
                     /locus_tag="PLL28_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNF68122.1"
                     /translation="MLRRLLPIIIFVIGADIVSIQAATNIFGSSTLLYGIWTGATVLV
                     LASLFLYFSREMRKRSGIARPVLMSTFFMLLFPKVIVSVLLLIEDVVRLTKLMANGVI
                     TALGSDFNFGLGRSFWWDAFALSFALLLTLAFVYGAIFNVYRYQIRKVKVKLVKLPKA
                     FDGLRVVQISDIHSGSLSNREAVAKAVDRINSLSPDLIFFTGDLVNNIAEEARGFVDI
                     FAKLQAKHGVYSILGNHDYGDYMAWPDEATKQHNLETLKTMHAQMGWRLLLNEHVHIE
                     RSGNKIAVAGVENWSARGRFKSYGRLEQTLHGIQDAMTVLLLSHDPSHWEGEVLDHPA
                     KVDITFSGHTHGMQFGLDLPWFKWSPVKYLYRQWAGLYQQNDQYLYVNRGFGVIGYPG
                     RVGILPEITLFTLQTTQSE"
     gene            9883..10410
                     /locus_tag="PLL28_02040"
     CDS             9883..10410
                     /locus_tag="PLL28_02040"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNF68123.1"
                     /translation="MIYSRYQQKLNRYETIVNELFLAAERQSASALNTPLQEGKWSTG
                     QQLFHVIRVSEVTMDYLEYKIQQQAAAPKAGISSFVRSTLLNIALILPIKFKAPAITA
                     AVGEDHTISDLTGYHQQQIIRIKAYLGQMTAADRKKALFKHPRAGRISMDQTLRFLAL
                     HARHHLKQVNKGINI"
     gene            10475..11215
                     /locus_tag="PLL28_02045"
     CDS             10475..11215
                     /locus_tag="PLL28_02045"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNF68124.1"
                     /translation="MTIFFVFFAIHWYLSLFSQTFFLHRYAAHSMFRMNPFWEKSFYL
                     FTWITQGSSYLSPYAYGILHRLHHAFADTEQDPHSPKYTKGIFPLMHKTKQIYSDINS
                     DTFPVDEKYKKNLPMWRSFDHFASSMIVRLLWVAAYFVFYLTFATHWWMWLLFPITVL
                     MGPVHGAIINYFAHKFGYRNFQVEDTSKNFLPVDVLMMGESYHNNHHKFGSRPNFGVR
                     WFELDPTYPLIKILHALHIIRLNKETAA"
     gene            11370..14729
                     /locus_tag="PLL28_02050"
     CDS             11370..14729
                     /locus_tag="PLL28_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68125.1"
                     /translation="MPGDTLLLDTLSIVPGSVIIQSLSIQSADTLYTVLDTNQFTISH
                     FNATIYFHPAPLGSICISYQVFPYLFTKKTYRYDSNAYDSTALNLSPYTIATNKQDVW
                     SAMSGLDYSGSFARGISFGNAQDVVVNSTFNLQLSGKLQNDVDISASITDNNIPIQPE
                     GNTQQLQEFDKVFIRLSKGGQQLTMGDFEISSKEGYFLQYYKKLQGASYSGNFTLNNG
                     STLQTGISAAVARGVYARQEISVIEGTQGPYKLKGNNGETFIIVLAGTERVYMDGSLL
                     VRGSEHDYVIDYNSGEVTFTPKVLLTKDKRVVVEFEYSDKNYFRTLVYSGTTWTSADE
                     RWKTRLQIYNEQDSKNQPLDQTLDANDRQALEAAGDSISTIYVPGIDSIAFDASRILY
                     RMVDSLGYDSVFVYSTDPDQAFYALAFTELGSGNGNYIEATTTTNGKVYQWVAPIAGV
                     PQGTAEPVILLIAPGKSRLITLGGEFNPDKKTKITGEFAISDNDLNTFSPTGNNDNKG
                     IAVRTGVSKAITSSTNNSLTIFANYEYAQSTFKTPERYRPVEFNRDWNIYSTEASDEH
                     YANAGVEWKPSDDISVGYTTSTFIRGLQYTGFLQDLHTQKTGKSWQWDAKAGLLTAAT
                     DSNNSIFFRPRINISKTFQQLNYVKTGIGYEGEHNAIKSIDDSLLATSFYYDQYNWFI
                     SNADTSKRYLRGEVIYRKDDAVFENKFRPVTEGITYQLQGRIGVHPQRSWQWQVYYRT
                     LSISDTSLTTQDTEQSLLGRLQQSSSFKKGLLTTDLSYEVGTGREPKRLYTYVETEPG
                     AGTFTWNDYNENGVQELNEFEIAVFADEANFIQVLIPTDDYIQTNSTAFNYSLGINPR
                     VLWNNAHGLEGMLGKCAVQSNLQMNRKVFDDQTISVYNPFASIDDTLLVSSNIYWVNT
                     LFINRSGSVLGADYSYNRNYNKQVITIGPESRGKEEHVINIRYRPWKPLITNITLKGG
                     VKDYYSPVFTEKNYGIDFREITPKITYNASNIFRTTLSFTYNESANGEGFEALISREA
                     SIDMKYSVVSKNTIQLKFSFVNNDFNGDADSPIGFAMLEGLQNGNNMIWSLNIDRKIS
                     QILQLNLGYEGRKTGDNEITHIGRVQMRAVF"
     gene            14781..15164
                     /locus_tag="PLL28_02055"
     CDS             14781..15164
                     /locus_tag="PLL28_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNF68126.1"
                     /translation="MKASTNSINWFEISVADINRAASFYSTIFDITMDRQEMMGMHMA
                     FFPWENGSGKANGALVQGDMYVPSADGVKLYLNADPDLQVVLDRIQGAGGQIVMAKTA
                     IGDGLGYMAFFVDTEGNVVGLHSNA"
     gene            15179..15613
                     /locus_tag="PLL28_02060"
     CDS             15179..15613
                     /locus_tag="PLL28_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HNF68127.1"
                     /translation="MDITINTPALLFPAISLVMLAYTNRFMALSSVVRKLHDEYEANR
                     SNKNLPLQIRNLQFRIKLVRRMQFFGVTSFIVALISMYLIYVDNLQMAHITFATAMLL
                     FACSLVISLIEIMKSTNALELELSDMEDEEGKSFVDYIKKHI"
     gene            complement(15825..16196)
                     /locus_tag="PLL28_02065"
     CDS             complement(15825..16196)
                     /locus_tag="PLL28_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68128.1"
                     /translation="MKHLCTLLIAIALLCGTANAQSIPDGTYVYTGLASNGHPLTFTA
                     TVSPTGLTFSEAIDGGVLCSANMVVESIDPSGTIFTYGTTTPGAADCTLGQDFIFMLT
                     PTPSILEFRKGEGPVFTLNLQ"
     gene            16364..18964
                     /gene="mutS"
                     /locus_tag="PLL28_02070"
     CDS             16364..18964
                     /gene="mutS"
                     /locus_tag="PLL28_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNF68129.1"
                     /translation="MKAVKETPLMGQYNRIKAKYPDAILLFRVGDFYETFSQDAVVTS
                     KVLGIVLTKRANGSASHIELAGFPYHALDAYLPKLVRAGYRVAICEQLEDPKLVKTIV
                     KRGVTELITPGVATNDKMLDTRSNNYLASYYGDKQMAGMSFLDISTGEFFVAQGGADY
                     FEKLLHSLQPAEIILPKQHQKAFKELYGNKFYIYAIDDWVYTEDYTKEKLLNHFGTIN
                     LKGFGIDDMPFGIMAAGASIHYLSDTEHNNIGHITGISRLSEEQSVWLDRFTIRNLEL
                     IHASSEDGSSLLKVLDHCITPMGTRLLKKYILMPLKDKHGIDARLDMVDHLLHDNELA
                     GVITNALKQIGDLERVVSKVSLQKINPREILQLNRALHACAPLRQALMQSNQPTLQKL
                     GDQIHLCELIRDKIDREIAEDAPAQVHKGGLIKQGVHAELDELRAIGSSGKDFLLRLQ
                     QDEIIRTGIPSLKIGFNNVFGYYLEVTNKYKDVVPSDWIRKQTLTNGERFITPLLKEY
                     EEKILGAEEKILALEQQIFDTLLSELQDYVRPIQINANVAARLDVLLNFATIANEYRY
                     QKPEITEDFGIDIELGRHPVIEHHLPIGEGYVPNSISLDTEEQQIVIITGPNMAGKSA
                     LLRQTALIVLMAQMGCYVPAQRARIGLIDKIFTRVGASDNISSGESTFMVEMNETASI
                     MNNLSARSLILLDEIGRGTSTYDGISIAWSLCEFLHQHPHARAKTLFATHYHELIELE
                     QKFDRIRNYHVSTREAGNKVIFLRTLQQGGSKHSFGIHVARMAGMPPQIVSRANEILK
                     QLEDKHIGVDLKNKLKKMQSQQFQLNMFSVDDPQMAKIRALLEETDINTLSPIEALMK
                     LKELRDLTGD"
     gene            complement(18992..19741)
                     /locus_tag="PLL28_02075"
     CDS             complement(18992..19741)
                     /locus_tag="PLL28_02075"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HNF68130.1"
                     /translation="MRTLGLILLMMLVQHNFAQVINIEDKRIKTDTVGWSGSGELSLY
                     ANKNNDMVLSLQTDLHLQYKTKKSLWLLLTDIATVQVNNEQHFVNSGFQHFRYNYKIR
                     DRFVWEAFAQGQYNEPLAIDYRLLAGTGPRFKLYGTDTFRCYVAALYMYEYEQNTGEI
                     DAQQNNRLSSYVSFTYSPSDHFSLVSTTYYQPKLDDYTDRRISTSLDVKSYITKKLYL
                     KLNYNLLDDSAPAEGVVNTIYEFTAGMGFDF"
     gene            complement(19768..22887)
                     /locus_tag="PLL28_02080"
     CDS             complement(19768..22887)
                     /locus_tag="PLL28_02080"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68131.1"
                     /translation="MRKSLLYLLLLSTISSFGQKNKQNSNLSLPFDTAITQHLNFRNV
                     GPWRGGRSTAVCGDPDDPQVFYFGATGGGVWKTQDGGNNWKNISDGYFGGSVGAISVS
                     KSDPTVIYVGLGENSMRGNVSEGTGIWKSMNSGRTWTYLGLPETRHIMRIVIHPKNPD
                     IVYVAAQGHLFGPNTDRGVFKTIDGGLHWQKVCYVDDKTGACDLVMDPGDPNILIAGF
                     WQVIRTPYSLESGGPGSGIYKSTDAGATWQNISHASGLPKDTLGIVGLAIAPSDPDIY
                     YAIIESKTGGVFRSEDAGKTWTKTSDNSNLRQRAWYFSKIYVDPKQSETVYVLNVEFW
                     KSTNGARDFKSIPTPHGDHHGLWIDDRDPQRMIVADDGGAQVSFNGGNEWSSYYNQPT
                     AQIYRVSTDNAVPYRILGAQQDNSSLRIRHRGTYGQIGNNDWQPTAGFESGYIVADPL
                     NPDIVYGGNYSGYIGCLNHDTRDTRNITVWPDDPIGQGAGDQAYRFQWNFPLFFSPHD
                     PNRLYAAGNVLFYTDDAGTSWHAMSPDLTTNTKSMQQPSGGIITKDNTGVEVYCTIFA
                     ASESGLEKGVIYTGSDDGLVYVTKNNGSDWQNITPKDLPPYTMINCIETDPIQKGKMY
                     FAATRYKLDDEQPLLYVTEDYGVTWKKIINGISPTHFTRVIRADRNREGLLYCGTERG
                     LYISYNGGNNWYPFQLNLPIVPITDMTIKDNDLIIATQGRGFWVLDNITMLQQMQAVN
                     NAFISYPVRNVYRMSMYKTEKPVNAGENPEPGVVFDMFIPAAKAKSGADSLSKCSIEI
                     KDHEGKSITTLATDAKEKSNTINIKPGFNRISWDMHYPGGKSIDGMFLWNGVPDGPLA
                     APGNYTAEINYGNTTTTQSFFIEPDPNSTATLDDYRLQHVFLMEMLETFNSTQEAILD
                     MQEMQKQMRDYLTKLDSLAPQSLKDSIANINKDLDAIINTLYETRNKSSQDMLNYGIK
                     LNDKLSGVIGAVSTGNFRPGNNQRMVYYDLKKQIDMQLEFYRDIKKNRISTLNKTINN
                     LALPVIITK"
     gene            22995..23082
                     /locus_tag="PLL28_02085"
     tRNA            22995..23082
                     /locus_tag="PLL28_02085"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23029..23031,aa:Ser,seq:tga)
     assembly_gap    23354..23453
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(23550..24185)
                     /locus_tag="PLL28_02090"
     CDS             complement(23550..24185)
                     /locus_tag="PLL28_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68132.1"
                     /translation="MKAFLRIFLILVVILLILAAAFIAKYPFYAPWYRAAHSAEDYKL
                     LVESTTKPESGVTLIQAFNDRYPYWLGTRWNFNGISEVPGSGSIACGYFVTTMMRDMH
                     FDINRVKLAQCASEEMIKALVDEKHIKRYNLVESDLMFDNIRKQKQNLYIIGLDNHTG
                     FVYNDGDDIWFIHSSGSFPYCVMKEDATACNTITDSRYKVLGCISQSDILF"
     gene            24265..24693
                     /locus_tag="PLL28_02095"
     CDS             24265..24693
                     /locus_tag="PLL28_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF68133.1"
                     /translation="MADIKTINNSSDIMHCFEVIHTLRPHLNEEQTIACFEQMRSEGY
                     HAVYVEDNGKAMAYCGYRFITHYFSGSAMYIDDLVTVADARNKGYASLLLDHVIEIGN
                     KKGLETIRLDSGHHRHDAHRLYLNKGFHIHSHNFSMTLKK"
     gene            complement(24695..26071)
                     /locus_tag="PLL28_02100"
     CDS             complement(24695..26071)
                     /locus_tag="PLL28_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNF68134.1"
                     /translation="MQYRLSFRHIIIIIGLSAIFASCNQDGNIFPTLDDQYDQYGSPF
                     DSVPETGNIVMYEINLRAYSAAGNIQSIIADLDRLKDISVNTIWLMPIYDEGELMAVH
                     SPYCIRDYYQVSDEYGSLQDLQQLTDAAHARNIAVVLDWVANHTSWDNDWITDHPDWY
                     TQDADGNIIHPPGTTWTDVADLNFDNQDMRAQMIDAMKFWVLVGNVDGFRCDYADGVP
                     FDFWQQALDSLRNIPDRTLIMLAEGDRDDHYTAGFDMTYAWDFYGTMQNVYSGSAVSD
                     LYATNNAEYAGVPAGDKKLRFTTNHDESAWNATPMVLFNGIDGAVAASVMTIFLNGVP
                     LIYTGQETGNTANVPFFSNAPINWTLNPEMQLEYKQILNAYSGHQTARTGTLTTFSNA
                     DVYCIKKTLASDQFVVLVNARSADVTYTLPTALKDTEWTDAINGNSVSLGTELALTPY
                     QYLLLTNE"
     gene            complement(26093..27175)
                     /locus_tag="PLL28_02105"
     CDS             complement(26093..27175)
                     /locus_tag="PLL28_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNF68135.1"
                     /translation="MRIGFDAKRIFQNATGLGNYSRAVVKNLASQFPENEYFLFTPNA
                     KDQLSFNAEGNIKTVIGRGSVWRSFGIRRDILKHKIDVFHGLSNELPFSINKADVKSV
                     VTIHDLIFDLFPHHYPGVDRTVYELKTKFAVNNADVVIAASESTRNDLVRMYGKDPAG
                     ISVVYQSCDDIFYAPPAADISSYRLPKQYMLYVGSVTERKNLLAVCNAYTKLPSADRL
                     PCVIVGNGKRYADEVRAFVSANHLTSSFIFLENVPTDHLPALYANALCFIYPSLYEGF
                     GIPILESMVSGCPVITSSVSSLPEVGGDAALYFNPENPEDIAAQIQRVVGDEQLRQNM
                     INKGKLHAQQFEKSKVTRQLMSIYKQ"
     gene            complement(27276..29387)
                     /locus_tag="PLL28_02110"
     CDS             complement(27276..29387)
                     /locus_tag="PLL28_02110"
                     /inference="COORDINATES: protein motif:HMM:NF013360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HNF68136.1"
                     /translation="MLKLCTPRIISALFLSASIAAATVSYAAPLMVGEDCNTAVSITE
                     GTYTAPNPDYWYSFTVPTTGTYVISTCDMGNTCDTRLYVYDHCVGLIPTELAEGTLSY
                     NDDYCGSQSQITTLLEAGDLVYIRIGDAETTCAGESINWSIAYTGLPTGCIDAFACNY
                     SPIAMVDDGSCIYPGNPDCPSGPDLVLDETYFDGDISTGWGTDFQLSTINADDWTNSC
                     YIDEGSLTGPGMRTIIRFGIKIWNLGDEDYHIGTAAENPYLVFDPCHGHTHYIDYGEY
                     LLYDSLGTEIPVGHKNGFAVMDLCGIGGYNGADMGISAGCFDAYGLGTGGQWIDITDV
                     PDGTYTFVARVNWENHPDIEGRVETNLANNWRSRCIHIWRDAGVINAEMIDDCPLYID
                     CLGEIWGTAQLDCEGNCNGWHHIGDLNEDSTRLYDDVDLYVNNILSNTIIADMCNDAN
                     NDSDIDVVDAALVMGCANQTYGYTHDTDLCELPVAMMNTSDTVTYSIGTVNAPFGYLD
                     IYSLNPSARVLGAQFTMEGITIDSVANLCPEVFGENYTLTYTDNEVIALGHNEVPYQI
                     HFEPIPTFRIFYTATYPTEICIKHFTAAVNEDFEEVVTALDNSCVTVDETGIQYYASN
                     PLEISIQPNPFSTAAELHFSNAGHQAFDLVITDINGNVVRTLNTTGNTITINRDELPA
                     GMYLYRLTGISESQSGKFVIN"
     gene            complement(29476..29841)
                     /locus_tag="PLL28_02115"
     CDS             complement(29476..29841)
                     /locus_tag="PLL28_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HNF68137.1"
                     /translation="MSLELFYLASCSTCQRIMKELKVQKYPFRLQDIKTEQITPGQLD
                     RMKDLAGSYEQLFSRVALKYRALGLNQKKLSEKDYKKYILEEYTFLRRPVFIIGEKIF
                     IGNAPSTVAAVAKELTALK"
     gene            complement(29872..30834)
                     /locus_tag="PLL28_02120"
     CDS             complement(29872..30834)
                     /locus_tag="PLL28_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNF68138.1"
                     /translation="MKIIPSNIRRLVANKYARVVNFTKRTSFPGFAGIPIYDVISFFW
                     QEIRRDQLPVRASAISFNFLLAIFPSIIFVFTLIPYIPIKGLDANIHDFFRDVLPESG
                     YAFLESTITGITSIKRGGLLSVGFILAFYFSTNGVRMLMLTFNKSHPIYQRRNFWRNR
                     IAAFRLTFYLFLLFIVSIITIVMGDKVVGVVGDWINWEDNTGVFFLNIFQYAIILILF
                     FFGISLIYYYGPAVKHRWRFITPGGTFATIVSIAASVIFGYAANNLIGYNEVYGSIGT
                     LIVLMIWINLNALVLLVGFEINNSVDVNRVLRSRDGEDEQHGTV"
     gene            complement(30865..32769)
                     /locus_tag="PLL28_02125"
     CDS             complement(30865..32769)
                     /locus_tag="PLL28_02125"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF68139.1"
                     /translation="MRTGIAIAIVCAFVSMLTFCTNNKGEATENVYLNANDTVSYVGM
                     ETCKLCHADKHATFMHTGMGMSFDKATKSKSAALFDAEHALVYDSTNNFYYKPFWRND
                     TLQIMEFRLDGQDTIHKRIEPIAFIIGSGQHTNSHMLQVNGYVYQAPITFYTQKQQWD
                     LAPGMSGGFNSRFSRVIEAECMTCHNGLPELVEGSVNKYAKVPSGIDCERCHGPGSLH
                     VARVSNGELVDTSKNIDYSIVNPRKLSVELQNQLCMRCHLQGVNVLNEGASFFDFKPG
                     DHINEHWNIFLPRFDGSNNKFLMASQADRLLQSKCYTSTHQLSCITCHNPHITVKDTP
                     REQFNAPCKQCHGEGNTQCKASIASRNNVADDCSSCHIRKSGSIDIPHVSISDHIISI
                     PKNTASKGSGTFKGLVCLTDSNPDPLTMARGYLHYYESFNKEKSLLDSAWQYIQKAGK
                     ENDEVRSVKVHYYYLKDAPDQIIQSGEKIKKDKLNAWTAYRMGEAYTAKSDFAAALKY
                     FNIAVEREPLNLDYLFKQATAMVFSGDNKSGEAVYRKIVGENPEYEKAWNNLGVILLT
                     KGQMPEAEQCLLKAIALNPDYTLSRIKLTELYIQSRQKSKAKSSLEYLQRYDADDPAV
                     ILLAQKLRSI"
     gene            complement(32812..33213)
                     /locus_tag="PLL28_02130"
     CDS             complement(32812..33213)
                     /locus_tag="PLL28_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HNF68140.1"
                     /translation="MTNQPIIFFDGVCNFCNASVNVILRSDKHAYFKFASLQSEFAKR
                     FFEEHPIAPNIDSIILWEDGKLFYETTALLRVAGKLAFPWKLFVAGWIIPRFLRDPLY
                     RFVAKRRYRWFGKRESCMIPTPDIAARFLDS"
     gene            complement(33210..33512)
                     /locus_tag="PLL28_02135"
     CDS             complement(33210..33512)
                     /locus_tag="PLL28_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNF68141.1"
                     /translation="MDISVEELKKRMDAGEDLLIIDVREPHEYAEFNIGAINIPLTSL
                     PMKMYDFDDDKDREIIVHCRSGMRSASAKALMVQSGFTNVRNLLGGMLEWQATVGK"
BASE COUNT         9995 a         6778 c         6961 g         9792 t
ORIGIN      
        1 atgttgccga caagcccacg ttcgctcagg ttgctgacga gttgaatgcc tatctcgaca
       61 actgtgattt agcaggatat aacagtaaca agtttgatgt gcccatgttg gtagaagagt
      121 ttcttcgctg cggaaagggt tttgatgaaa atcgcaggta tattgatgtg atgcgcattt
      181 tcacgctgat ggaaaagcgc accctggagg ctgcctatcg ctttttctgc aataagactt
      241 tagaaaatgc ccacagtgcc gaagcagatg ttaccgctac ctacgaggta ttgctgggtc
      301 agctcgataa atatggcgat gcgcttgctc atgatgtccc ttccttgcat gagttcacga
      361 aggatggcga ttttgtggat tttggtcgca gaatgatcat gaaaaatggc gtgccatgtt
      421 ttaattttgg taagtacaat ggtcagtcgt gtgaagacgt ttatcgacgt gaaccgcagt
      481 atttcgattg gatatataaa agtgattttc ctgaacatac caaactcaag ctgcggctga
      541 tagcattaaa aattaaaaac gacaaaaaca aagcttcctg atcccttggc acgcgcactt
      601 gtaattatac caacttacaa cgagcaggca aatatcgccg atatgataac ggctgtattg
      661 tctttgccgc aggattttga tttgttaatc atcgatgata attcacctga tggaacggca
      721 tcagcagtga cagcgatgca atcaacctac cctacccgac tgcacttaca tcaacgcgcc
      781 ggcaagttag gattaggcac tgcttatatc gccggattca aatggggctt agcgcaaggc
      841 tatgattata tgatagaaat ggatgccgat ttttcacata agcccgaaga tcttcccagg
      901 ttattggacg cctgtaaaaa tggtgccgat gtggcaatag gttcacgtta tattaaaggt
      961 ggcaatgtag aagactggcc aaccgatcgc attttattat cgaaatgggc ttcgcgttat
     1021 gtgcgcatga taacatggat acctgtgcat gatactacag ccggttttgt ttgttacaaa
     1081 cgcaatgtac tggataccat agatctggat aaaatcagat ttattggcta tgcatttcag
     1141 atagaattaa aatacgctgc atggcgcagt ggattctcat tgaaagaaat acccattgtg
     1201 tttaaagacc gccagaaggg gatttcaaaa atgagcaaag gcattttcaa ggaagccgtt
     1261 tatggtgtaa tcaccatgcg atggaagagc ttctttcatt cttataccaa ataagatttc
     1321 atgtcatcgg aacaacgcca taagcgaaaa ggcattcata gaaaaaaatc gggattacca
     1381 ccgggcacgc ctatttatac cggtgcgaaa aagcatgaaa taattacatt acagcgtttt
     1441 gcctatgata ctgatacaat agaagaaatt aattgtaagg caggtgataa gattgatcta
     1501 atacccagag aaggaaaagt attatgggtt aatattaatg gtcttcatca ggaagcgctg
     1561 atagaagaaa ttgccgctac attcaagttg cacaatctgg tattagaaga tattttaaat
     1621 gtattccaga tacctaaagc ggaagaatat cccgatacag atcagattta cattgcgtta
     1681 aacgaatttt attttgctgc tgaaggcaaa ttggaacgcg atcagataag catcatttta
     1741 ggcaatgatt atgtactcag ttttcaagaa gaagagggtg attattttga attgattcgc
     1801 gacaggttgc gcaatgctaa aggcaaactg agaaagaaat ccggcgatta tcttgtttac
     1861 gttttaattg attccgtagt agatagttat tatgttgtat tagatcatta tgcaacggaa
     1921 ctcgacagta tagagaatga cattttcgaa aagaaaaatc gcaatcactt agaacgtatt
     1981 catcaactca acaaagactt gatttacttg cgcaggtcga tagcgccatt gaaagaggtg
     2041 ctgttcaaat tgttgaaaga cgacgtgata ctcattaatc ctgagtcgaa gctctttttg
     2101 cgcgatgtac tcgaccatgt gaatcaggtt gtcaaccaga ttgatgtaga ccgcgaatat
     2161 ctggcagatc tggtgcagac agatatggcg aatatgaatg cccatttgaa cgagatcatc
     2221 aaggttttga cgctcatttc aacgattttc atacccttaa ccttcatagt tggtgtttat
     2281 ggcatgaact ttggaaatat cccattaatg cagaccactt acggctattt tgtcgtatgg
     2341 ggtgttatga ttttcattac cctgctgcag tttttcattt tcaaaagaaa aaagtggcta
     2401 taagcatcat ccaatcacac aaaaaaacag cttatgcgtt taacattacc cattttcatt
     2461 tcagcctgta ttttgacatc aaacttgcaa gcgcagagta ccattgcacc accagccaac
     2521 acctcggttc tttttaatat taatttcgat cttggcattc cgatgaatga attcaaaacc
     2581 tacaacgatg aagttgctgc aggtggtgga ttcagtttgt tctttcaacc gtcaaataaa
     2641 atacctgttt tggtaggctt tgatatggca tttatgggca acggacataa aacacagcat
     2701 gaaacactta ccgcagatat tgtggcaggt acaactgtga tagaaacgct ttatttcccc
     2761 atgcgtgttg aaacctataa tatgatcaca accggtgatg tgcatttgcg ggtgcaatcg
     2821 ccaaccaatt tctttaagcc atatttggat ggtatggtag gattcaatca cttcggaacg
     2881 aacacctctg tttatgatga gtctgaggaa tattatttga gtgaagagga caatccattg
     2941 atcaccagtt ctcagcaaaa ttcaagttgg tcgctgaact acggtggcgc cttaggtatg
     3001 ttggtgcaat taaatgaaaa cattttcctt gatgtgcgtt gtgcctatac atttggcggc
     3061 gaagctgaat attatgtgga agatgacatt gaaaactggg aaattgagtt taatactgtt
     3121 ccgacaagtc aggatgatgt atctgatgaa gatatagata tttcagccat caaaaagcaa
     3181 tcgcatacag atatgatcat gggtaccata ggactaacct ttaagtttta atcacattat
     3241 ataccaatat acaacgctat cctaaatcga tagcgttttt tttgtttata tatgattatg
     3301 taaattcact acctttcgca tctatacaaa gaacacaaat aactacgttg atagcatatg
     3361 cggtccggac gcaggaagtt atcgcacaag gtgcatctgg tttacggctt ggtggtgatc
     3421 atagctgtgg gcattgcatt ttttattctg cacaacctaa aagcatcaaa cgccagtcaa
     3481 ttaaacgatt cctctgcaac cccggtcatc gattcgttgc atcatattcc ggtgtattat
     3541 aatggtgatg tagaccatgt tgcaggtcgc agtgttgcgc gcaatggata taactacggg
     3601 ttaaaatggc aatgcgtaga atttgttaaa cgatattatg cagatcatct gggacactat
     3661 atgcccgatg ctgatggtaa tgcgaaggac ctttttgaca agcgtctgcc tgatgcctca
     3721 tttaatgaag cacgtgcatt agtgcaatat gccaatccaa gtcttaaaaa accgcaaccg
     3781 gatgacataa ttgtttttga tggccattta ggcaatcgct acggacatgt ggcaataatt
     3841 agtcaggtta ctgaaacaag tattgaaatt atacaacaga atgccggccc tgacacacca
     3901 acacgcgagt ggctttcatt agaaaaagtt gacaatcgat gggaagtaga tcacaagcat
     3961 gtattgggat ggctgagggt tgaataaaaa aaacaccgca ggtttctcag gcctgcggtg
     4021 cgaaagcaat actttgcttc ggtctataaa tgcgcttatt tgtctgaatc atcatcgtcg
     4081 aaattcatcg actgcaccaa ctgcggaata tcttctactt tcagacggca atgcattttc
     4141 aggtaaacaa atgtttcttc tttttcatca accaatagca ccattcgttt tattttatca
     4201 tgcttatttg aacgaataga aatattcacg gtttcctcat cagtccgcac gctcataagt
     4261 tgttcataat cgcggcgttc catgcgatca agtttgcgtg tgattttttt atcagtgcga
     4321 tcggcataag cttctccttc gcggatgcaa atatttatac ttccgaattt gcgtagcagc
     4381 gcgccggtgg cagcaccttc agcatccata tgatcttcaa caatatcggc ggctatgcgc
     4441 attaccacac cgggaatcca aaggtttaat tttgttgatt catcttcagg ttcaggtaaa
     4501 gaagcggcat gagcggtatt aaatccgact gcagcgcaaa atacgagggt gatcagcttt
     4561 ttcattgtaa taggttgatg cggtgataat tatttagttt cttctacttt cttgagttca
     4621 tcaagacctt cgatatcgat atcggagatt tgagaaattt tgctcagatc gatagcaccg
     4681 agtatgctga tcagtacaaa ttcaccatcg gcatcgcaaa ccatcaacat atcgttgagc
     4741 gtgctaccgg tgatattttt accatacaat ctcacctttt ctccttctgc atttacgttc
     4801 atcaattctt cataaccatt tgttcctacg gtggtcataa attcgttgta ataagcgccg
     4861 gaatttgaag caccttctgc attttcatat acaagaatgc gaattccttt aataccatcg
     4921 atcatatttt tcagatcagc atcatcggta gttgcagaag caacaagaga gaataatttt
     4981 tcagtaacga ttacagaagt aaatccctct tttccttcat attttttaaa gaattgatct
     5041 atctgtgcag attgagcgaa agccattgca gaaatcatca atgcgcaggc ggtaaatagc
     5101 atctttttca tttgtagccg tttaggtggt gattaaataa ttttgaacat taggcgaatg
     5161 aggttcgcaa aaggttacag ctcgggctta aaaaaaatga aataaatcgt cagaatgctt
     5221 gcctatcaag ccttacggct gataaaaatt accaggagca acatctggtt gtagaggtta
     5281 ttgagcctta cggaaaggtt taccggggct gtttcgaggt tgctataaag ttttggaagg
     5341 ttttttaaat ccctgatttt cccttctatc gcatacataa aacgctcaac gttccaacct
     5401 atatatttct cttgttgctt ggaaagaata actgcagcgg gcaatttttt tggtccttca
     5461 caaagtgtgt caaatttata attagcacta taagcttcag caatagcaga agcagacaat
     5521 ccatcgatca tcgccattac atttacacct gatttatcgc gatgttcgag caatttcata
     5581 ttgtcgcgca actcacgaag cagtgcattt ttaattctgt cgcgcgatgt tgatctgtcg
     5641 gcaagaaact gaaataattc ctttactgta gttattccgg tttgcaggta gtcggccatg
     5701 gcgatgaagg tacaacagaa aattcaatta gccgtgatta cacgtcttat ttcgaggaag
     5761 tgtttattat agtatttctc ggttgcttca ttactaatgc gcactccacc ggtttcagga
     5821 gatgaagtat gtataaatga aaagccgttt tctgcatcat agtccagcac gatacctaca
     5881 tgtccgggat ctccgggaac aactgttgca ctgctgaaaa cgatgatatc tcccgggcgt
     5941 gcatcaacgt aaggaattgg aatgccagca tcgaattgca tatcgctggt gcggggcaag
     6001 gtgatattaa aagccttgta tacatagcaa ataaatcccg aacaatcgaa gccatgttga
     6061 ggatctgtgc caccccatac ataaggagtg ccgataaatg ttttggcata agcaataatg
     6121 ctatcggctt tagccagctc tttaatttca gtagaatcta cctgctgttc ttgccaccag
     6181 ggagtgagtt gggcatgcat ttttgtacta aaaaatacca tgcatacaca acacactatg
     6241 cgggcaagat tattcatcag ggatgctggc ttttcttttt cttcttcttt ttagcatcgc
     6301 gctcccacca tacaggtgtt tgatcttccg ggtcgagatt ataatttacg gtaatctctt
     6361 caccgggttc gatatcgcgc actgcctgga attcgagtac gttgagatcg aaatcaggga
     6421 tatatcgcgc atttggagaa tagctatgat tgtataatga accaaatcct aaagcgagag
     6481 cacctttttt tcccttcttc ccccactcga aataatattc gtaaaggaaa gtatcgtcaa
     6541 tactcaagcg atctttttcg ctgaatacta tcattggcgc cacttccagg gtatcgccgg
     6601 cttttacggc ttttgaggta aacacggcac gtcctttatc gggagtgcgt gccacaaata
     6661 aatgatcagg caacacgggt gtgagtttcc tcactaccac ttcttctttt ttttcttttt
     6721 tcgacatcac ctgattattt agcggaaact acatcctcca actgtttctt cacatcggca
     6781 aaaagggcag taaaatgttt ttttacagca ctttttcctt tcaccttgct gctgccattg
     6841 atttttttca cggcagcttc atatgtatta aaaagtgcat ttattttaga ttccaattgt
     6901 tcatcggtga tatccatgaa caacacacgg tcgatcattg atgcaacggc agcgccgatt
     6961 cttttcttag cattacgttt actcatgtca atattttcag caaagttaag catatggcta
     7021 tctttgcgca ataaatacct tgtctatgtt tgatatcaag aaggctatgg agatgaaaaa
     7081 gcagatggaa gagatccaga agcgattgga taacatcact gtggagggcg tttcgggtga
     7141 tgggaaattt caggtcgtta ttacgatcac cgcgaataaa aaggtcaaat ctgtgcatat
     7201 cagcgatgaa ttgatggcat caggcgacaa agaacacctc gaggacctgc ttgttatagc
     7261 gatggagcgc ggtttagaca aggcgacaca ggtatcggag gctgaagctc ggaatgtggc
     7321 tttatcggga gggttgaacg gtttattcgg gtcctgatca gacgagttct tctactttga
     7381 agatggcgct ttttacacgt ttaccggcgt catcagacaa tatcagctct ttcgcagacg
     7441 gtagttcaaa cacctgttgt aagtccctga tagcacctac aggaacatga ttttgacggc
     7501 ataattcgag gaaactatcg cttgaatatg actgcacttt ggattgcagc actcctatca
     7561 acgtatttct gttgtgcacg cgatcggtat tatgcgcata gcgttggtcg gcatatacct
     7621 caggacagtt aagaatatcg cataaatttt tgaattggcg gtcggaaccg attgccagca
     7681 ccacctgcac gccatctttg caagtgagaa tctcgccata gggagcgata ttaggatgca
     7741 gagaaccatt gcgttgcggg atatggccgg cagtgatata attcgcagcc tgattggcaa
     7801 gcgccgcgat ggaagcatca aatagcgata cactcacgcg acttcctttt cctgttttat
     7861 acttttgaat gagggctgtc aggatgcctt cctttaattg atgggaagca aataaatcga
     7921 taatagctac gggaagttta acgggaggtg agctaggggt gccattcatg aacatccaac
     7981 cggtttctgc ctgcaggaca acatcaaaag caacgcgatc ctcttcatcg ccaaaaccgt
     8041 tgatattggc ataaataatt ccggggttca gcgccgccac ggtgttagga tctagcttga
     8101 atttctgtgc atctcccttt ttccagttgc atatcatgac atcagcctgt ctgatcatat
     8161 cataaacctg ctccagacat gaagggtctt caaggtcgag gaagaggtgg agtttgcgcc
     8221 aattgactgc tgcgaaataa gctgaggttg aggcggcagg attttcgctt ggaagcttcc
     8281 actgtctggt gatatcgcca ccggtgcgag gattttcaat tttcactaca tcagcaccca
     8341 attccgaaaa aaatgaaccc gcagcaggcc cggccattac acctgccaat tcaattatcc
     8401 tcaatccttt aaacatgcat caaaattaca aggaaataac aatgttaact ttttataaat
     8461 aacgataaaa gtaaggcaag cgttggcagc aaagtcgaag aaaaggccga ttttcttaca
     8521 tatgcgactg ctaatcatgg ttttagaggt aaaaatgtcc ggtttttagt ggtttatttc
     8581 gatatcctta caatattaag tctttgccgg cgtcagaacc aaacagtaaa aatgacgtta
     8641 acattaccat atgctaaggc gattattacc tattatcatt tttgtcatcg gcgctgacat
     8701 agtatctatt caggccgcta cgaatatttt tggatcctct actctacttt atggcatttg
     8761 gaccggtgca acagttttgg ttttggccag cttgtttttg tatttcagtc gcgaaatgcg
     8821 caaacgaagc ggtatagcga gacctgtttt gatgagtact tttttcatgc tattgtttcc
     8881 aaaagttatt gtttccgtcc tgttattgat agaggatgtt gtaaggctga caaagctgat
     8941 ggcaaatggt gttattactg ctcttggatc ggattttaat ttcggattag ggaggtcatt
     9001 ttggtgggat gcatttgccc tttcatttgc gttattattg accttagcat ttgtttatgg
     9061 agcgatattc aatgtgtatc gctatcaaat tcgaaaagta aaagtgaaac tggtgaaatt
     9121 gccaaaagcg tttgatggct tgcgtgttgt gcaaatttct gatatacatt cgggtagttt
     9181 aagtaatcga gaggcagttg caaaagctgt tgacaggata aactctttat cgccggattt
     9241 gatttttttc acgggtgatc tggtaaataa tattgcggaa gaggcgcgcg gatttgtaga
     9301 catattcgcc aagttgcagg caaagcatgg cgtttacagt atacttggca atcatgatta
     9361 cggcgactat atggcatggc ctgatgaagc taccaagcag cataatttag aaacgctcaa
     9421 aaccatgcat gcgcaaatgg gttggcgatt gctcttaaat gaacacgtgc atatcgagcg
     9481 atcaggaaat aaaattgcgg tagcaggagt agaaaactgg agtgccaggg gtaggtttaa
     9541 aagttatggc agattagaac agacattgca tggtattcag gatgccatga cggtattatt
     9601 gttatcgcat gatccgtcgc attgggaggg tgaagtattg gaccatcctg ccaaagttga
     9661 cattaccttt tcgggacata cccatggtat gcaatttggg cttgatcttc catggtttaa
     9721 atggagtccg gtgaagtatc tctatcgtca gtgggccgga ttgtatcaac agaatgatca
     9781 atatttatat gtaaatcgag gttttggtgt aataggttat ccgggcagag ttggaatttt
     9841 accggaaata accttattta cattgcaaac tacacaaagc gaatgattta ttcccgctac
     9901 caacaaaaat taaatcgcta tgaaactata gtgaacgaat tatttctggc agcagaaagg
     9961 caatctgcat cagcgttgaa tacaccgtta caggaaggaa aatggagtac cggccagcag
    10021 ctatttcatg tgatacgtgt ctcggaagtt accatggatt acctggagta caaaattcaa
    10081 caacaagcag ctgcgccaaa ggccggaata agtagttttg taaggagcac tttattgaat
    10141 attgcattga ttcttcctat aaaattcaaa gcgccggcaa taactgctgc tgtaggagaa
    10201 gatcatacta tttcagattt gacgggttac catcagcaac aaatcattcg aattaaagca
    10261 tatctgggac aaatgactgc agctgatcgc aagaaagcgt tgttcaagca tccgcgagcg
    10321 ggcagaatct caatggatca gaccttgcgg tttttggcct tacatgcccg tcatcatttg
    10381 aaacaagtaa acaaggggat caacatttga gtaaacctct gtttttaata caaatgccgt
    10441 aattttacgc ccgatttcac ttatcgatca ttacatgacc atcttttttg tgttttttgc
    10501 gatacattgg tatctgtcac tattcagcca aacatttttt ctgcatagat atgccgcgca
    10561 cagcatgttt agaatgaatc cgttctggga aaagtctttc tatctgttca catggataac
    10621 acaggggtcg agttatttga gtccgtatgc ttatggcatt ttacatcgtt tgcatcacgc
    10681 ctttgcagat actgaacaag atccgcattc gccaaaatat actaaaggca ttttcccgtt
    10741 gatgcataaa acaaagcaga tttatagcga tatcaattca gatacctttc cggttgatga
    10801 gaagtataaa aagaatcttc ctatgtggcg cagttttgat cattttgcca gtagcatgat
    10861 cgtgcgttta ttgtgggttg cagcttactt tgttttttat ctgacatttg ccacacattg
    10921 gtggatgtgg ttattatttc ccattaccgt tttgatgggt ccagtgcatg gtgctatcat
    10981 caactatttt gcacacaaat ttggttatcg caattttcag gttgaggata cctcaaaaaa
    11041 tttcctcccg gtagatgtat tgatgatggg tgaaagctac cataacaatc accataaatt
    11101 cgggtcacga ccaaactttg gagtacgttg gtttgagttg gatccaacct atccgttgat
    11161 taaaattttg cacgccctcc acattattcg cttaaacaaa gaaaccgctg cctaggcatg
    11221 caacattctt tcgggtagat tcgttgtaaa tttacctgaa aatcaggctt atccgtcgcc
    11281 ttgcagctat actatgtatc ttttgcggac tgtttttccg acagcccctg catgctcaat
    11341 ggtccaatga gcgggtgcgg tatattgaca tgccgggaga tacgctactt ctggatacat
    11401 tgagtattgt tcccggcagt gtaatcatac aatcattaag catacaatcc gccgatacgc
    11461 tttatactgt tttagatact aatcaattta ccatttcaca ttttaatgct acaatttatt
    11521 ttcatcctgc acccttagga tccatatgca ttagttatca ggtattccct tatttattta
    11581 cgaaaaaaac ttatcgttac gacagcaatg cttatgatag cacggcgctc aatttatcgc
    11641 cctacactat tgctaccaac aaacaagatg tatggtcagc aatgtcggga ttagattatt
    11701 caggaagttt tgcaagaggg atatcatttg gcaacgcaca agatgtggta gtaaattcaa
    11761 cctttaattt acagttgtca gggaaattgc aaaatgatgt tgacatcagt gcatcaatta
    11821 cagataataa tattcccatt cagcctgaag gcaatacgca acaattgcaa gaattcgata
    11881 aagttttcat acgattatcc aaaggtggtc aacaacttac catgggtgat tttgagatca
    11941 gtagtaaaga gggctatttt ttacaatatt ataaaaagct gcaaggagca tcctactccg
    12001 gaaatttcac gctcaataac ggtagtacat tgcaaacagg cataagtgca gctgttgcac
    12061 gtggagttta tgcgcgacaa gaaatcagcg tgattgaagg cacacaaggg ccttataagt
    12121 tgaagggaaa taacggcgaa acttttatca ttgtgttagc agggactgag cgtgtgtata
    12181 tggatggcag cttgttggtg cgaggttcag aacatgatta tgtaattgat tataattcgg
    12241 gcgaagtaac tttcactccg aaggtattac ttacaaaaga caagcgcgta gttgttgaat
    12301 ttgaatattc cgacaaaaat tatttcagga cactggtgta ctcaggaacc acttggacaa
    12361 gtgccgatga gcgatggaaa acacgcttac aaatttataa tgagcaggac agcaaaaatc
    12421 aaccacttga tcaaaccctg gatgccaatg accgacaagc actggaagct gcaggtgata
    12481 gtatatctac tatctatgtc ccgggaattg acagcatagc ttttgatgct tcgcgcattt
    12541 tatatcgcat ggttgacagc ttgggttatg acagtgtttt tgtttattca accgatcccg
    12601 atcaagcgtt ttatgcactg gcatttaccg aattaggctc aggcaatgga aactatattg
    12661 aagcaacgac aactacaaat ggaaaggtgt atcaatgggt tgcaccgatt gccggcgtgc
    12721 cacaagggac tgcagagccc gttattttgc tgatagctcc gggcaaatca agattaatta
    12781 cgttaggagg agaatttaat cccgacaaga aaacaaagat caccggtgaa tttgccattt
    12841 cagataatga tttaaatacg ttttcgccga cgggaaataa tgacaataaa ggtattgccg
    12901 tgagaacagg tgttagcaag gcaatcactt catcaacaaa taattctttg accatttttg
    12961 ccaattatga atatgcacag agcaccttta aaacacctga aagatacagg cctgtagaat
    13021 ttaacagaga ctggaatatc tatagcacag aagcttcaga tgaacattat gcaaatgccg
    13081 gcgtcgaatg gaaaccaagt gatgatattt cggtaggcta tactacatcg acatttatac
    13141 gcggactgca gtataccgga ttccttcagg atttacacac acaaaaaacc ggcaaatcct
    13201 ggcaatggga tgcgaaagca gggttactga ctgcggcaac agatagtaat aacagcattt
    13261 tctttcgccc acggattaat atcagcaaaa cttttcagca acttaattat gtcaaaacag
    13321 gcatcggata tgaaggtgaa cataatgcca tcaaaagtat tgacgatagt ttactggcaa
    13381 cttcatttta ttacgatcaa tacaattggt tcatctcaaa cgcagatacg tcgaaacgat
    13441 atttgcgcgg tgaagtgatt tatcgcaagg atgatgccgt atttgaaaat aaattcagac
    13501 cggtaaccga aggaataacc tatcaacttc agggaagaat tggtgttcac ccacaacgtt
    13561 cttggcagtg gcaggtttat tacagaacct taagtatttc agataccagt ctaaccacgc
    13621 aagatacaga gcaatcattg cttggtcgat tgcaacaaag cagttcattt aaaaagggat
    13681 tacttaccac cgatttatca tacgaagtag gaaccggcag agagccaaaa cgcctgtata
    13741 cttatgtaga aacagaaccc ggcgcaggaa catttacctg gaacgactac aatgaaaatg
    13801 gtgtgcagga attgaatgag tttgagattg cagtatttgc cgatgaagcc aattttattc
    13861 aagtattaat tccaaccgac gattacattc aaacgaatag cacggcattt aattattcgt
    13921 tgggaataaa tccgcgtgtc ctatggaaca atgcacatgg attagaaggc atgcttggga
    13981 aatgcgctgt tcagtctaat ttacaaatga acagaaaagt attcgacgat caaacaattt
    14041 cggtatataa tccttttgca agcattgatg acacattgct ggtgagttca aatatatatt
    14101 gggtcaatac cttatttatt aatcgcagtg gtagtgtttt gggggcagat tattcataca
    14161 atcgcaatta taataaacaa gtaattacta taggtccgga aagtcgaggg aaggaagagc
    14221 acgtaatcaa cataagatac cgaccttgga aaccattgat aacgaatatc acattgaagg
    14281 gaggggtaaa agattattac agtcctgttt tcacagagaa aaattatgga atagattttc
    14341 gcgagatcac accaaagatt acatacaatg caagtaatat tttcaggact acactctcct
    14401 ttacctataa tgaaagcgct aacggagagg gatttgaagc attgatcagt cgggaagcga
    14461 gcattgatat gaaatatagt gtagtaagta aaaacacgat tcaattaaaa ttcagttttg
    14521 taaataatga tttcaatggc gatgcagatt cacccatcgg ttttgccatg ttggagggat
    14581 tgcagaatgg taacaatatg atatggagtt taaatataga tcgtaaaata tcgcagatct
    14641 tgcaattgaa cctaggctat gaaggtcgca aaacggggga taacgagatc acacatattg
    14701 gaagggttca aatgcgtgca gttttctgac aaattttgtt tagggaaaat tgcctacttt
    14761 agactcataa attaaatcaa atgaaggcat caacaaattc aatcaattgg ttcgagatat
    14821 cagtagcaga catcaaccga gccgcaagtt tttattcaac tattttcgac atcaccatgg
    14881 acaggcagga aatgatgggt atgcatatgg cctttttccc ttgggagaat ggcagtggaa
    14941 aggctaatgg agctttagtt cagggcgaca tgtatgttcc aagtgccgat ggcgttaaat
    15001 tatatctaaa tgccgatccg gatcttcagg tggtattgga ccgcatacag ggagccggag
    15061 gacagatagt tatggctaag acagccattg gcgatggttt aggatacatg gcttttttcg
    15121 ttgatacgga agggaatgta gtaggattgc attcgaacgc ataatcgtaa ttttgatcat
    15181 ggatattacc attaatacac cggcattatt atttcctgcc atcagtttgg ttatgttggc
    15241 atatacgaat cgattcatgg cattgagtag tgttgttcgg aaattacatg atgaatatga
    15301 agccaataga tcgaataaaa acttaccatt acaaattcgc aatctgcaat tccgtatcaa
    15361 actagtcaga cgtatgcaat tttttggcgt gacaagtttt attgtggcgc tcataagcat
    15421 gtatctaatt tatgtcgata acctgcaaat ggcacacata acatttgcaa ctgccatgtt
    15481 attgtttgcc tgcagccttg tgatttcttt aattgaaatc atgaaaagca ccaatgcact
    15541 cgagttggaa ttgtcggata tggaggatga agaagggaaa tcttttgtcg attacatcaa
    15601 gaagcatatt tgatctaata aaaaagcccg gctaaccggg cttttttatt gcaggtatga
    15661 aaatgctttc taggtttagg gatagcagtg catttgttgg aataattacc ttttaattaa
    15721 atcaacaatg tgaaggtaat gggggtgcac tatccgacaa acccactttt agcgagccag
    15781 ccaattttta atgcgaaata gccgaataat tgtgtaaacc gaaattattg caggttcaaa
    15841 gtaaataccg ggccttctcc ttttctgaat tccagtatag aaggtgttgg tgttaacatg
    15901 aaaatgaaat cctgccccaa tgtacaatct gcagctccgg gggtagtagt accataagta
    15961 aagattgttc cagatgggtc gatagattca accaccatat tcgcactaca tagtacacca
    16021 ccgtcgatgg cttcagaaaa agtcaatccg gttggtgaca cggtagctgt aaatgtcaaa
    16081 ggatgtccat tagatgccag tccggtatat acataagttc catcaggaat gctttgtgca
    16141 ttggcggttc cgcacaataa ggctatggca atgaggagtg tgcagagatg tttcataagc
    16201 gctttttggt gtttggtatc taaagataag gcaatttcaa ttcagaggca acaaccggaa
    16261 ttgttaatgc tgtaggcaag gcgattgtgg aaaaccttat aaaattgggg aaaaccttgt
    16321 ggcagtaggc tttttcggga tgtagcggct atatttgcag gctatgaaag cggtgaagga
    16381 aactccatta atgggacagt ataacaggat aaaggccaaa tatccggatg ctattttact
    16441 gtttcgtgtt ggagatttct atgagacctt ttcacaggat gccgtagtaa ccagtaaagt
    16501 tttgggtata gtactcacca agcgggcaaa cggatctgca agtcatatcg aattagcggg
    16561 attcccctat catgcgctgg atgcctattt accgaaactg gtacgggcag gatatcgcgt
    16621 tgccatatgc gaacagcttg aagatccgaa actggtgaaa accatagtaa aacgtggcgt
    16681 tacggaattg atcacgccgg gagttgccac taacgataaa atgcttgata ccagaagtaa
    16741 taactacctg gcaagttatt atggcgacaa gcaaatggcg ggaatgagtt ttctggatat
    16801 cagcacggga gaattttttg ttgcgcaagg aggtgctgat tattttgaaa agctactaca
    16861 tagcttgcag cctgctgaaa ttattttacc taagcaacat caaaaagcat ttaaagaatt
    16921 atatggcaat aagttttata tctatgccat tgatgattgg gtatataccg aagattacac
    16981 caaagaaaaa ctgcttaacc atttcggcac aattaatctg aagggattcg gcattgacga
    17041 tatgcctttt ggaattatgg cagccggagc atccatccat tacctcagtg atactgaaca
    17101 taataatatc ggacacatca caggtatctc aagactatcg gaggagcagt cggtgtggtt
    17161 agatcggttt accatccgca atctggagtt gatacacgcg agttcagaag atggatcaag
    17221 tttactaaag gtgcttgatc actgcattac gccgatgggc acgaggttgc tgaaaaagta
    17281 tattctcatg cctttgaaag ataagcatgg catcgatgca cgtttggaca tggttgatca
    17341 tttactgcac gacaatgaac ttgccggagt tatcaccaat gccttaaaac aaattggtga
    17401 cctggaacgt gtggtgtcta aggtttctct tcaaaaaatt aatcctcgtg agattctgca
    17461 gttgaatcgc gccttgcatg catgtgctcc gttgcgacag gcgctcatgc aaagcaatca
    17521 gcctaccctg caaaagcttg gcgatcagat acatttgtgt gaattaatca gagataaaat
    17581 cgatcgtgaa attgctgaag atgctccggc tcaagtacac aaaggcggac tgataaagca
    17641 aggtgttcat gccgaattag atgagttgag ggccattgga agttcgggga aagatttttt
    17701 acttcgttta caacaagatg aaattatcag aaccggtatt ccaagtttga agatcggttt
    17761 taataatgtt ttcggatatt acctcgaagt aaccaataaa tataaagatg tagttccatc
    17821 agactggata cgcaagcaaa cactgacgaa tggtgaacgg tttatcactc cattactaaa
    17881 ggagtatgaa gaaaaaattc tcggcgcaga agaaaaaatt ctggcattgg aacagcagat
    17941 attcgatact ctgttatccg aattgcagga ttacgttcga cctattcaaa taaatgcaaa
    18001 tgttgctgcg cgattggatg tattattgaa tttcgcaact atcgccaatg aatatcgcta
    18061 tcagaaacct gagatcacag aagattttgg tatagatata gaacttggtc gccacccggt
    18121 aattgaacat catctgccaa ttggcgaagg ttatgtgccg aattcaattt cattggacac
    18181 agaggaacaa caaattgtaa tcattaccgg tccgaacatg gcgggtaaat ctgcgctact
    18241 gcgtcaaacc gcacttattg tattaatggc acaaatggga tgctatgtgc ctgctcaaag
    18301 agctagaatc ggattgatag ataagatatt tacaagagtt ggtgcaagtg ataatatttc
    18361 ttccggcgaa agcactttca tggtagaaat gaatgaaact gccagcatta tgaataattt
    18421 atcggcccgt agtttgatct tacttgatga gataggcaga gggaccagta cttatgatgg
    18481 catatctatt gcctggagcc tatgcgaatt tttacatcaa catccacatg cgcgtgcgaa
    18541 aacattattt gcaacgcatt accatgagct aattgaattg gagcagaaat tcgatcgcat
    18601 tcgaaactat catgtaagca cgagagaagc gggtaacaaa gtcattttcc ttcgcacatt
    18661 acaacaagga ggcagcaaac atagttttgg cattcatgtt gccaggatgg cgggaatgcc
    18721 accacagatc gtaagcagag caaatgaaat attgaagcaa ctggaagaca agcatattgg
    18781 cgtcgatctg aaaaacaaat taaaaaaaat gcaatcacaa caatttcaac tgaatatgtt
    18841 tagtgttgac gatccgcaga tggcaaagat tcgggcatta ttagaagaaa cagacataaa
    18901 caccctctca cctattgagg ccttgatgaa attaaaggaa ttacgcgatt tgaccggaga
    18961 ctaattatcc gcaatcaact actctacagc atcagaaatc aaatcccatt cctgctgtaa
    19021 attcataaat ggtgttaacc actccttctg cgggcgcact gtcatcaagc aggttataat
    19081 tgagtttcag atataatttt ttggtaatat aagatttcac atcaagcgat gtcgagattc
    19141 gcctatcagt ataatcatcc agtttgggct gataataggt tgtcgacacc agtgaaaaat
    19201 ggtcactcgg agaataggta aatgacacat aactactcaa tctattattt tgttgagcat
    19261 caatttctcc tgtgttttgt tcgtactcgt acatatagag tgccgcaaca tagcacctga
    19321 aggtatccgt tccatacaat ttaaatcgag gtccggttcc ggcaagcaac cggtagtcta
    19381 tagccagcgg ttcattatat tgaccttgcg caaatgcctc ccatacaaat ctatcacgaa
    19441 ttttatagtt gtatctaaaa tgctggaatc cgctatttac aaaatgttgt tcattgttaa
    19501 cctgcactgt tgcgatgtcg gtaagcagta accaaaggct cttcttggtt ttgtactgta
    19561 ggtgaagatc tgtttgcaac gacaacacca tgtcattgtt tttattggca taaagcgaca
    19621 attcgccgct gcccgaccaa ccaacagtat ctgttttgat gcgcttatct tcaatgttta
    19681 tcacttgagc aaaattgtgt tgcacaagca tcatcagtaa tatcaggcca agggttcgca
    19741 tggttaattt ttacaaaatt aatctaatca tttagtaatg ataacaggta atgcaagatt
    19801 gtttatcgtt ttgtttaaag tgctgatcct attcttttta atatcccggt aaaactctaa
    19861 ttgcatatca atttgttttt tcaggtcata atacaccatt cgttgattgt tgccgggcct
    19921 gaaattaccg gtagaaacag cacctatcac accgctgagt ttatcattta atttaattcc
    19981 ataattcaac atatcctgac tgctcttatt tctggtttca tataaggtat ttattatggc
    20041 atcaaggtct ttgttgatgt tagctatact atccttcaaa gattgggggg ccagggaatc
    20101 cagtttagtc aaataatcac gcatttgttt ttgcatttcc tgcatatcca ggattgcctc
    20161 ttgcgtagaa ttaaatgttt ccagcatttc catcagaaat acatgttgca atcgatagtc
    20221 atcaagtgtt gcagtggaat tgggatcagg ttcaataaaa aaagactgtg tagttgtagt
    20281 gttgccataa ttgatttcag cagtataatt tccgggagcg gcaagtggac cgtcagggac
    20341 gccattccac aaaaacatgc catctattga tttaccaccc ggataatgca tatcccagga
    20401 aattctgttg aagcccggct taatatttat cgtattggat ttctctttcg catcggttgc
    20461 cagtgtcgta atggatttac cttcatggtc tttaatttct attgaacatt tgcttaatga
    20521 gtctgctccg gattttgctt ttgcagcagg aataaacatg tcgaaaacta ctcccggttc
    20581 aggattttcg cctgcattta ccggtttctc tgttttatac atcgacatac gatatacatt
    20641 tcgaaccggg taagagataa atgcattatt tactgcttgc atttgttgta acatggtaat
    20701 attatccaac acccagaatc cacgaccctg cgtagcaata attaggtcat tatctttaat
    20761 agtcatgtca gtaatcggca ctatcggcaa gtttaattgg aaaggatacc aattatttcc
    20821 cccattataa gaaatataca acccgcgttc cgtgccacaa tatagaagtc cttcgcgatt
    20881 tcgatcagca cgaattactc tggtaaaatg agtaggtgaa attccattga taattttttt
    20941 ccaggtaaca ccataatctt cagtgacata taacaaaggt tgttcatcat ccagtttata
    21001 tcttgtggcc gcgaaataca ttttgccttt ttgaatcgga tccgtttcaa tgcaattaat
    21061 cattgtatac ggaggcaaat ctttaggagt tatattttgc cagtcgctgc cattattctt
    21121 tgtaacatat accaatccat catcactgcc ggtataaata acaccttttt caagtccgga
    21181 ttctgacgcc gcaaaaatgg tgcaatacac ttccacacct gtattatcct ttgtgataat
    21241 gccgccgctt ggttgctgca tggattttgt gttggttgta aggtcgggcg acatagcgtg
    21301 ccatgaagtt ccggcatcat ctgtgtaaaa caacacatta ccggctgcat acaatcggtt
    21361 aggatcgtgt ggtgaaaaaa acaatgggaa attccattgg aagcgatagg cttgatctcc
    21421 cgccccctga ccaattgggt catcaggcca aacagtaata tttctggtat cgcgtgtatc
    21481 gtgattcaaa cagccgatat atcctgaata atttccacca taaacgatat caggatttag
    21541 tggatcagct acaatataac cactttcaaa tcctgcagta ggctgccaat cattattacc
    21601 aatttgccca taggttccac gatgccttat gcgcaatgat gaattatctt gttgggcacc
    21661 aagaattcga taaggaacag cattatctgt agaaacccga taaatctgag cggttggttg
    21721 attataataa ctactccatt catttcctcc gttgaatgaa acttgtgcgc caccatcatc
    21781 agcaactatc atgcgttgag ggtcacgatc atcaatccat aaaccatgat gatcgccgtg
    21841 gggagttgga attgatttaa aatcacgagc accatttgta cttttccaga attccacatt
    21901 cagtacataa actgtttcgg attgtttagg atctacataa atcttagaaa aataccatgc
    21961 acgttgacgg aggttgctat tgtcgctggt tttagtccat gtttttccgg catcctcgct
    22021 tctgaataca cctccggttt tggactcgat gatggcataa taaatatcag gatctgaagg
    22081 tgctatcgcg agtccgacaa ttcccaaagt atccttcggt aaaccgcttg catgtgaaat
    22141 attttgccag gtagcgccgg catctgtaga cttataaatt cctgatccgg ggccaccact
    22201 ttcgagtgaa tagggtgtgc gaatcacctg ccagaatcct gcgatcaaga tgttaggatc
    22261 tcccggatcc atcactaaat cgcaggcgcc tgttttatcg tcgacatagc atactttctg
    22321 ccaatgtaaa ccaccatcaa tggttttgaa tacacctcta tcggtattgg gaccgaaaag
    22381 atgtccctgt gctgcaacat acacgatatc cgggttttta ggatggatta caatacgcat
    22441 gatatgcctt gtttccggta acccgagata agtccatgtt ctgccgctgt tcatgctttt
    22501 ccaaatgccg gttccttcgc tcacattacc gcgcatgcta ttttctccta aaccgacata
    22561 tatcactgtg gggtcggatt tacttacgga tattgctcct accgagcctc cgaagtagcc
    22621 atcagaaatg tttttccagt tgttaccacc atcttgagtt ttccaaactc ccccacctgt
    22681 agctccgaaa tagaacactt gcggatcgtc cggatccccg caaacagcgg tagaccttcc
    22741 acctcgccaa gggcctacat ttcggaagtt aaggtgctga gttatcgcag tatcgaaagg
    22801 tagcgacaga ttgctattct gtttattttt ctgcccgaag gatgaaattg ttgacagcaa
    22861 aagcaagtac aataaggatt tacgcataag acggtataag ctgctaatta cgtcattacc
    22921 cgttaaaaat caaagcttta tgcaatgttt caagactatt tccattcaac aaaaaatcgt
    22981 catatctttg cgccggagag atggcagagc ggtcgattgc ggcggtcttg aaaaccgttg
    23041 aggtgcgagc ctccgggggt tcgaatccct ctctctccgc caaacaaatt atatgcagcc
    23101 taatgtaggc tgcatttttt atttcaaaag ccagtgacaa gtttacttgg cgctggcttt
    23161 tgaaataaaa aatgcaggtg agcgcagcga atggctgcat gaaattggtt tgaagccccc
    23221 catcttaggg atcatggtaa taatccctca gttcaataac ttcaacatca acttaactgc
    23281 aaactcattc ctatatcaaa gtttacttgg cgctggcttt tgaaataaaa aatgcaggtg
    23341 agcgcagcga atgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgcatga
    23461 aattggtttg aagcccccca tcttagggat catggtaata atccctcact cctttcaatc
    23521 atgcttaaaa gtaaatttgc aacactcatt caaaaaagaa tgtcactctg agaaatacat
    23581 ccaagtactt tatatcgtga atcagtaata gtgttgcaag cagttgcatc ttctttcatc
    23641 acacaatatg gaaaggaccc gctgctatgg atgaaccaaa tgtcatcgcc atcattgtaa
    23701 acaaatccgg tatgattatc gagtccaatg atatataaat tctgtttctg tttacgaata
    23761 ttgtcaaaca tcaaatcact ttctacaaga ttatatcgct tgatatgttt ttcatctacc
    23821 aacgctttta tcatttcctc tgatgcacat tgggcaagtt tcaccctgtt gatatcgaag
    23881 tgcatgtcac gcatcatagt ggtaacaaaa taaccgcaag ctatgcttcc cgagccgggc
    23941 acttctgaaa tcccattgaa attccagcgg gtgcctaacc aatatggata acgatcgtta
    24001 aatgcttgaa ttagtgtgac gccggattcc ggttttgtgg tgctttcaac caatagttta
    24061 taatcttcgg cgctatgagc tgctctatac catggtgcat aaaatgggta cttggcaatg
    24121 aaagcggcgg ccagtatcaa taagattaca actaggatga ggaatatgcg aagaaatgct
    24181 ttcatgagtg tgtattcgtg aagataacga atatttaaat ggtgatatta tagattctcc
    24241 tacctttggc ataaatcaag cgcaatggca gacatcaaaa ccattaataa cagcagtgat
    24301 atcatgcact gttttgaagt gatccatacc cttagaccac acctaaatga agaacagaca
    24361 attgcttgtt ttgagcagat gcgctcagaa ggttatcacg ccgtgtatgt tgaagataac
    24421 ggcaaagcaa tggcatactg cggttatcgg tttatcactc attactttag tggatcagct
    24481 atgtatatcg acgatctggt aacagttgcg gatgcaagaa ataaaggata tgctagtttg
    24541 ttgcttgatc atgtgattga gataggcaat aaaaaagggt tggaaacgat cagattagat
    24601 tccggacatc acagacacga tgcacatcga ttatatctga ataaaggatt tcatattcat
    24661 tcccataatt tcagtatgac gctaaaaaaa taaactattc gttggtaagt agcagatact
    24721 gatatggcgt cagtgcaagt tcagtgccaa gtgagaccga atttccgtta atagcatcag
    24781 tccattcagt gtcttttaac gcggtaggca atgtataggt aacatctgcg gaacgcgcat
    24841 tcaccaagac aacaaattga tcgcttgcca aagttttttt aatgcaataa acatctgcat
    24901 tgctgaatgt ggtaagtgtt ccggtacgcg cagtctgatg tccactataa gcatttaata
    24961 tctgtttata ttctaactgc atttctggat tcaaagtcca atttatagga gcgttggaaa
    25021 agaatggcac atttgcggtg ttacccgtct cttgtccggt atagatcagc ggtactccat
    25081 ttaaaaatat ggtcatcaca gacgccgcta cggcgccatc aatgccattg aaaagcacca
    25141 tcggagtggc attccaagcc gactcatcgt gattcgtagt aaagcgcagt tttttgtccc
    25201 ctgcaggcac accagcatat tctgcattat tagtggcata caaatcagat acggcagagc
    25261 ccgaataaac attttgcatg gtgccataga aatcccatgc ataggtcata tcaaagcctg
    25321 ctgtgtaatg atcatcgcgg tcgccttcag ctagcatgat caatgttcga tcaggaatat
    25381 tgcgcaaaga atctaaagcc tgctgccaga aatcaaacgg tacgccatcg gcataatcac
    25441 atctgaaccc atcaacatta ccaaccaata cccagaattt cattgcatca atcatttgag
    25501 ctcgcatatc ctggttatca aaattcaaat ctgccacatc ggtccacgta gttccgggtg
    25561 gatgaataat attaccatca gcatcttgtg tataccaatc gggatgatct gtgatccagt
    25621 cattgtccca tgaggtatga ttagcaaccc aatcaagtac tactgcaata tttctggcat
    25681 gagctgcatc tgtcaactgt tgaagatcct gtaatgatcc atattcatca ctaacctgat
    25741 aataatcgcg aatacaatac ggtgaatgta ctgccatcaa ttcaccttca tcataaatcg
    25801 gcatcaacca tatggtattc accgatatat cctttaagcg atcgagatct gcgattatag
    25861 actgtatatt tccggcagca ctatatgcgc gcaaattgat ctcatacatg acgatgtttc
    25921 ctgtttcagg aacactatca aatggtgatc catattgatc atattgatca tcaagtgtcg
    25981 gaaaaatatt gccatcctga ttacaagatg caaatattgc gcttagccct ataatgatga
    26041 taatatgtct gaatgacaac ctatattgca tggcgaaagt atttcgcttc aattattgtt
    26101 tatagataga cattaactgt cgggtaactt tcgatttctc gaattgttgt gcgtgcaatt
    26161 tccctttgtt aatcatattt tgacgcaatt gttcatctcc tactacacgt tgaatttgag
    26221 cagcaatatc ttcagggttc tcgggattaa aatacaaagc tgcatcgcca cctacttcag
    26281 gaaggctgga aacagaggac gtaataacag ggcagccact caccatggat tcaaggatag
    26341 ggatgccgaa tccttcatac aaactcggat aaataaaaca cagcgcatta gcgtacaaag
    26401 caggcaaatg atctgttggt acattttcaa gaaaaataaa tgatgaagtc aagtgattcg
    26461 ctgacacgaa ggctcgcact tcatcagcat atcgcttgcc attaccaact atcacacagg
    26521 gcaaacgatc agcagaaggt aattttgtat atgcattaca aactgcgagt agattttttc
    26581 tttcagtaac ggatcctaca taaagcatgt attgttttgg caatcgataa cttgaaatat
    26641 cagctgccgg cggtgcataa aaaatatcat cacagctttg atacacaaca gatatacctg
    26701 caggatcttt tccgtacatc ctcaccaaat cattgcgtgt actttcactt gctgctataa
    26761 caacatcagc attatttact gcaaattttg ttttcagctc ataaacggtt ctatctacac
    26821 cgggatagtg atgagggaac agatcgaaaa taagatcgtg aatggtaact acacttttaa
    26881 catcagcttt attgatgctg aatggtaatt cattactcaa gccatgaaac acatcaattt
    26941 tatgtttcag gatatcccgt ctgataccga agctcctcca aacagatcct cggccaatga
    27001 cagttttgat attcccttca gcattaaatg aaagttgatc tttagcattc ggggtaaaca
    27061 aaaagtattc attctccgga aactgagagg caaggttctt cacgactgca cggctgtagt
    27121 tccccaagcc ggtggcattc tgaaaaatcc ttttagcatc aaatccaatt ctcatcagta
    27181 gtaacgggta tccacaacga aggtaatgaa gaatgtgaca aacataaaaa aaacccctgt
    27241 tgatgacagg ggttctagat atggtcaata agttatcagt tgatcacaaa tttaccggat
    27301 tggctttcac ttattccggt gaggcgatat aaatacatac ctgcaggcag ttcatcgcga
    27361 ttgatggtga tagtattgcc ggtagtatta agtgtgcgca caacatttcc attgatatcg
    27421 gtgattacaa ggtcaaaggc ctggtgtccg gcattgctga aatgcagttc tgccgctgtg
    27481 ctgaaaggat tcggttgtat gctaatctca agcgggttgc tggcataata ctgtatacct
    27541 gtttcatcca ctgtcacaca gctgttatca agggctgtca ctacctcttc gaaatcttca
    27601 ttcactgctg ctgtaaagtg tttgatacat atttcagtag gatatgtagc agtatagaaa
    27661 attcggaagg ttgggatagg ctcaaagtga atttgataag gcacttcatt atgaccaagt
    27721 gcaatcactt cattgtcggt ataagtgagc gtataatttt caccaaatac ttcaggacaa
    27781 agattagcca ctgagtcaat agtaatacct tccattgtaa attgtgcacc taaaactctg
    27841 gcacttggat tcaagctata aatatcaagg tatccgaatg gtgcatttac cgttcctatg
    27901 ctgtaagtta cggtatcaga tgtgttcatc attgcaaccg ggagttcgca gaggtctgta
    27961 tcatgtgtat aaccatacgt ttgattagcg cagcccataa cgagtgcagc atccactaca
    28021 tcgatatcgc tatcattatt tgcatcgtta cacatatcag cgatgatggt atttgagaga
    28081 atgttattta cgtacaagtc aacatcatca tacaaacggg tgctatcctc attcaaatcg
    28141 ccaatgtgat gccaaccgtt acagttgcct tcacagtcga gttgtgctgt tccccaaatt
    28201 tctccgagac aatcgatgta cagcggacaa tcatctatca tttctgcatt gataacacct
    28261 gcgtctcgcc agatatgaat acaacgtgaa cgccagttgt tggctaggtt ggtttcaaca
    28321 cgtccttcga tatcagggtg attttcccaa ttcacacgtg ctacgaaagt ataagtgcca
    28381 tcaggaacat cggtgatatc aatccactgt ccgcctgtac ctaatccgta agcatcaaag
    28441 caacctgcag aaatacccat atcagcacca ttatatccac cgataccgca aagatccatc
    28501 acagcaaaac cgtttttgtg tccaacagga atttcagttc ctaaagaatc atataacaaa
    28561 tattcgccgt agtcgatata atgtgtatgt ccatggcaag ggtcaaatac caaatatgga
    28621 ttttctgcag ctgttccgat atgataatct tcgtcgccca gattccagat ttttattcca
    28681 aaacgaataa ttgttctcat accgggaccg gtaagtgaac cttcatcaat ataacaggag
    28741 ttcgtccagt catcggcatt tattgtcgaa agctgaaaat ctgttcccca gcctgtgctg
    28801 atgtcaccat caaaatatgt ttcatccaat accaggtccg gtccggaagg gcaatcagga
    28861 tttccgggat agatacaaga gccatcgtca accattgcta taggtgaata gttacaggca
    28921 aaagcatcaa tacaacctgt aggcaatcct gtataggcaa tcgaccaatt aatggactcg
    28981 ccggcacagg tcgtttcagc atcaccaata cgaatgtata ccaaatctcc tgcctcaagc
    29041 aaggtagtta tttgcgattg agatccgcaa taatcatcat tgtaggataa agtgccttct
    29101 gcaagctctg ttggaattag tccaacacaa tgatcataga catagagcct ggtgtcgcag
    29161 gtgtttccca tatcgcaggt agagatcaca taagttccgg tagtaggaac agtaaagcta
    29221 taccagtaat cagggttagg tgccgtgtag gttccttccg ttattgaaac ggcggtatta
    29281 caatcctctc caaccatcag aggcgcagca tatgaaacag ttgctgcagc aattgatgct
    29341 gaaaggaaca aggcagaaat aatgcgagga gtacatagtt taagcatacg ggtcgatttt
    29401 ctccgataaa tatacagaaa ttccggcttt tgaagcggac tcagggtaag gattgagcca
    29461 aaaaaggtct tagtttcatt ttaaagccgt taattcctta gccaccgctg caacagtgga
    29521 aggggcatta ccaatgaaaa tcttctctcc gatgataaaa acaggcctcc ttagaaacgt
    29581 gtattcctca agaatatatt tcttgtaatc tttctcggaa agcttctttt gattaagccc
    29641 caatgcccga tacttcaggg caaccctgct gaacaactgc tcataactgc cggctaagtc
    29701 cttcattctg tcaagttgcc cgggcgtaat ttgttctgtt ttgatgtctt gcagtctgaa
    29761 aggatatttc tgtactttca gctccttcat tatacgttga caagtgctgc atgatgccag
    29821 ataaaataat tcaagcgaca taaatagaaa acaatagcat tattaaatgg tttaaacggt
    29881 tccatgttgt tcatcctctc catcccgaga acgaagcaca cggtttacgt caacgctgtt
    29941 atttatttca aatccaacca gtaataccaa cgcattaaga tttatccata tcattaaaac
    30001 gatcaatgtt ccgatagatc catacacctc gttgtatccg atcaggttat tggcagcata
    30061 tccaaatatt acagatgctg cgatggaaac aattgttgca aatgtgcctc cgggtgtaat
    30121 aaagcgccaa cgatgtttta cagccgggcc gtaataatat atcaatgata tcccaaaaaa
    30181 gaagaggata aggatgatag catattgaaa aatgttgaga aagaacacac ctgtattatc
    30241 ttcccagttg atccaatcgc caaccacacc cactacctta tctcccataa cgatagttat
    30301 aattgaaacg ataaacaata aaaataaata aaaggttaat cggaaggcag ctattctgtt
    30361 gcgccagaaa tttctgcgtt gataaattgg atgcgattta ttaaatgtga gcatcaacat
    30421 gcgcacgcca tttgtagaaa agtaaaatgc taagataaaa cccacagata acaatccgcc
    30481 acgctttata cttgttatac cggtaatggt agactccaga aaagcatacc cactttcagg
    30541 caatacatct ctaaaaaaat catgaatatt tgcatctaaa cctttaatag gaatgtaagg
    30601 tattaaagta aacacaaaga tgatagatgg aaatattgct agcaggaagt tgaatgaaat
    30661 cgcagatgct cgaacaggta attgatcacg tcgaatctcc tgccagaaaa agctaataac
    30721 atcataaatg ggaatacccg caaaacctgg aaagctggtc cgcttggtaa aattgaccac
    30781 acgtgcgtat ttattagcta cgagtcgacg aatgtttgag ggaatgatct tcatacgctg
    30841 tggttccgac acaaaggtat gattttatat ggatcgtagc ttctgtgcca gtaggatgac
    30901 cgccggatca tcggcatcat atcgctgcaa atactccaaa gatgactttg ctttcgactt
    30961 ttgccgcgac tgtatatata attccgtgag tttgatgcgc gaaagggtat aatccgggtt
    31021 caaggcaata gcttttaata agcactgctc cgcttccggc atctgccctt ttgttaataa
    31081 tataacacca agattattcc aggctttctc gtactccgga ttctccccta ctatcttccg
    31141 atatacagct tcacctgatt tattgtcacc actaaatacc atagccgttg cttgtttgaa
    31201 cagataatca aggtttaatg gctcgcgttc tactgcaata ttgaaatatt ttagtgcagc
    31261 agcaaagtca cttttcgcag tataagcctc tcccattctg taagcagtcc atgcgtttaa
    31321 tttgtctttc ttaattttct ctcccgattg aataatttga tccggtgcat ctttcagata
    31381 ataataatgc acttttaccg agcggacttc atcattttct tttcctgctt tttgaatgta
    31441 ttgccaggca ctatcaagca aactcttttc cttattaaac gactcataat aatgcaggta
    31501 accccgcgcc atggttagcg gatcaggatt gctgtcagtt aaacacacca aacctttaaa
    31561 tgtcccgcta cctttagatg ctgtattttt tggtatgctg atgatgtgat cagaaatgga
    31621 cacgtgtggg atatcaatgg agccgctctt ccggatatgg caactactgc aatcatctgc
    31681 cacattatta cgcgatgcta ttgatgcttt gcattgtgta tttccttcgc catgacattg
    31741 cttacaagga gcattgaact gttcgcgagg tgtatcttta accgtaatat gcggattatg
    31801 acatgtgatg caactcaatt gatgtgtaga agtataacat ttcgattgca gtaaacgatc
    31861 tgcctgcgac gccatcaaaa atttattatt actgccatca aatcgcggaa gaaaaatatt
    31921 ccagtgctca ttgatatggt ctccgggttt gaaatcgaaa aatgatgcac cttcattcaa
    31981 gacattaacg ccttgtaaat gacatcgcat gcataattga ttttgtaact ccacagataa
    32041 ttttcttgga ttcacaatgc tgtagtcaat atttttcgat gtatcaacca attcaccatt
    32101 acttactctg gcgacatgca gacttcccgg accatgacaa cgttcgcaat cgattcccga
    32161 aggcaccttt gcatatttat ttacagatcc ttcaactagt tcaggcaatc cattgtgaca
    32221 ggtcatacat tcagcttcta tcactcttga aaaccttgag ttgaatccac cgctcatgcc
    32281 cggagcgaga tcccattgct gtttttgtgt gtagaaagtg attggcgcct gataaacata
    32341 tccattcact tgcagcatat gcgagttggt atgctgcccg ctgccaatga tgaaggcaat
    32401 tggttctata cgcttgtgaa tggtatcctg accatcaagt ctgaattcca ttatttgcaa
    32461 ggtatcattt cgccagaatg gtttgtaata aaaattattc gtgctgtcgt acaccaatgc
    32521 atgttcagca tcgaacaaag cagcactttt cgatttcgtc gccttatcga aactcatacc
    32581 cataccggta tgcataaaag ttgcatgttt atcggcgtgg catagtttac atgtttccat
    32641 gccaacatac gagactgtat cgtttgcatt gagatacaca ttttcggttg cttcaccctt
    32701 gttgttggtg caaaaggtaa gcatactgac aaatgcacaa acgatagcta tggcaatgcc
    32761 tgttcgcatg atgatgtaaa aataggtgat gcaatcgttg cttacaacaa atcagctatc
    32821 cagaaatcga gctgcaatat ccggcgtagg aatcatacac gactcccgct tcccaaacca
    32881 gcgatagcga cgctttgcaa caaaacgata caggggatct ctcaggaacc ggggtattat
    32941 ccatcctgca acaaacagtt tccaagggaa agccagttta cccgccacgc gaagcaatgc
    33001 tgttgtctcg taaaataatt taccatcctc ccacaagatt attgaatcaa tatttggggc
    33061 aataggatgt tcctcgaaaa agcgctttgc gaattccgat tgcagcgaag cgaatttgaa
    33121 atacgcatgc ttatcacttc ggagaataac attcaccgag gcgttgcaga aattacatac
    33181 accgtcaaaa aaaatgatgg gttgatttgt catttaccaa cagtagcctg ccattcgagc
    33241 atgccaccaa gcaggttgcg aacattagta aaacccgatt gcaccatgag tgcttttgca
    33301 gatgcagagc gcataccgga gcggcaatga acaatgatct cgcgatcttt atcatcatcg
    33361 aaatcataca tcttcatagg aagcgatgtg agcggaatgt ttatcgcccc aatattaaac
    33421 tctgcatatt catgcggctc gcgcacatca atgatcagca ggtcttcacc ggcatccatt
    33481 ctttttttga gttcttctac agaaatatcc atgtcctgaa aattgcctga aattattgtt
    33541 taacaaatgt ggttgatgca agcattgcct tttgcgcatc taaaattttg agtatatata
    33601 ttcctgccga taaatcagca atattt
//
LOCUS       DAOQHY010000013        31911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_592_length_31911_cov_5.205832, whole genome shotgun sequence.
ACCESSION   DAOQHY010000013 DAOQHY010000000
VERSION     DAOQHY010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 31911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31911
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_592_length_31911_cov_5.205832"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..2715)
                     /gene="cphA"
                     /locus_tag="PLL28_02140"
     CDS             complement(67..2715)
                     /gene="cphA"
                     /locus_tag="PLL28_02140"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNF68142.1"
                     /translation="MKILSTAVMKGPNYWSIRRHKLIVMKLDLEELEEMPTNKIHGFR
                     ERLEQIIPSLYEHRCSEDHAGGFFKRVDEGTWMGHVVEHIALELQTLAGLDTGFGRTR
                     STSTKGVYNVVYSYLEEQTGIYAGKIAVELCESLVRGEFYDIAPHIMKMKEIRERERL
                     GPSTGSIVEEAIKRDIPYIRLNRRSLVQLGWGKNQRRIQATVASTTSNIAVEIACDKE
                     ETKNLLQQLNIPVAKGGSAYDEDDLEAIIKRIGYPIVIKPIDGNHGRGSTMNIKNWEE
                     AVAALKKSKEVSRWAIVEQYIVGYDYRLLVINYQFVSAALRKPAAIIGDGVHTVKQLV
                     DMVNADPRRGYGHENVLTSIKIDDHTLRLLEKLGMTEESIPSPGEEIFLKSTANLSTG
                     GTATDVTDIVHPYNVFTAERIARTIGLDICGIDIMSPDISVPMTENRGVVLEVNAAPG
                     FRMHLAPSEGIGRNVAEPVVDMLFPAGTQGRIPIVAITGTNGKTTTTRLCAHIAKTMG
                     HKVGFTTSDGIYIQNRMLEKGDCTGPVSTKFVLMDPTVDYAVLECARGGLLKAGLGFD
                     KCDYAIVTNVTADHLGLKDIDTVEEMAKVKSVLPETVHKNGYAILNADDDLVFNMRKN
                     LECKIALFSMDENNSRIMEHCERGGICAVLENGWITILKGNWKLRVEKVVNIPLTFDG
                     KATFMIQNILPATLCAYLQGFKVEEIKLALQTFIPGPATTPGRMNIFKFRNFEVLVDF
                     AHNPDGFQAISNYLSKVNAPIKVGLIAAAGDRRDEDIRELGRIAARTFDEIVIRQDKN
                     LRGRTDEEIMNLLMDGILEIKPGLDVIRINPEKESIKYVIENAKPGSHITICSDVIAE
                     ALDLVLQYKEKDDQFEFNKGEIPNVH"
     gene            2910..3725
                     /locus_tag="PLL28_02145"
     CDS             2910..3725
                     /locus_tag="PLL28_02145"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HNF68143.1"
                     /translation="MIPKGRLIAVGGAEHRDHIEQDKLEVLRRILQEMRGPDTVLEII
                     PTASGIPKQIAREYTDAFGTLGCKATRIMNIRKQKDAQKKEFLDRLRSCDGIMFSGGN
                     QTLLSETFLGTEFLDILKSRYINEPGFVIAGTSAGAMAQSGSMINGGAPAEALMRGKA
                     LMIEGLGFTNNSIIDSHFVNRGRFGRLMVAVAEHPQLTGIGISEDTAVIIKEDRYLEI
                     IGTGLVVIMDGTELRLNNIANNKGEMVNLERMIFHLLSKGRHYDMQERKVITN"
     gene            complement(3722..4645)
                     /locus_tag="PLL28_02150"
     CDS             complement(3722..4645)
                     /locus_tag="PLL28_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HNF68144.1"
                     /translation="MNAPIRIAIHGGAGTMPKRLMTPEIEAACHDSLQLALQAGYSAM
                     AKGGTAVDGVEAAVRALENAPMFNAGKGSVFTNKGQHEMDASIMNGETLDCGAVAGVS
                     NIKNPVTLARCVMDNSEHVLLLNDGAEEFARLHNIVFEDDDYFFTQLRYDQWQKALKK
                     KITVLDHVDDKKFGTVGAVAIDAKGNLAAATSTGGMTNKLYGRVGDSPLIGSGTYANN
                     ASVAVSCTGHGEFFIRQVVAYDLHCLMTYKGMGLEEAANHIVHDKLKPIEAEGGLIAI
                     DAKGNICLPFNTPGMYRGWMKSEADYFTGIY"
     gene            4791..5492
                     /gene="msrA"
                     /locus_tag="PLL28_02155"
     CDS             4791..5492
                     /gene="msrA"
                     /locus_tag="PLL28_02155"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNF68145.1"
                     /translation="MKGTKTITAALLSLVLGITGCGAKQATSDNQQIQDNKQSNDLAM
                     RKTYTDEEIRNNGWEKATFGGGCFWCTEAIFEEVKGVKSVVSGYAGGKMEKPTYEAVC
                     TDTTGHAECVQVVFDPKECAFATILDVHMKTHDPTTLNRQGNDVGIQYRSVVFYENDA
                     QKTATEDYINKLNASGYYSDKIVTEVVPLTTFWPAETYHQDYFAKNPDQPYCVYVVAK
                     KVDKFEHLFPELIEK"
     gene            complement(5500..6339)
                     /locus_tag="PLL28_02160"
     CDS             complement(5500..6339)
                     /locus_tag="PLL28_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68146.1"
                     /translation="MRIIILHLILCITSSFVNAQSITLGPVEANDTSSLTTSDDKLQE
                     GNYYKYFSINLKAGDGLLVYMESSSFIPFVTLINEDTTGFVQGWIEQIKSDVFGSYIN
                     FTTLADTTVFILLTSKESYQTGTFRYVVKQFSGDQLFYDKQMPDCRKLVYLLNQWNAD
                     WKMFPITTVLNEETYEEVSTFENTFFNGTSGRLIRGKYTEVYSELASEALAISQFDSL
                     TTTFSECLLNSNFNHSSSTDGPVKTEWFTINGFSNSTHAASFSLQLDLSNESAPKMIL
                     TLY"
     gene            complement(6352..6741)
                     /locus_tag="PLL28_02165"
     CDS             complement(6352..6741)
                     /locus_tag="PLL28_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002517182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNF68147.1"
                     /translation="MPEHVTKTRIDKWLWAVRLYKTRSLATEAVKNGKVKVNGDSVKP
                     SFEVFPGLILTIPKGVVKYQYKVLAPLERRVGAPLVKDYMEDVTPEEEKMKLTTLEFM
                     PTAFREKGTGRPTKKERRDLDDWMETE"
     gene            6809..7156
                     /locus_tag="PLL28_02170"
     CDS             6809..7156
                     /locus_tag="PLL28_02170"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HNF68148.1"
                     /translation="MQKPISAREIVLSLKDRFRPEKAEAGYETVFHLDISGDRGGQFT
                     VTIANGTINIEDGFHGNPKCIVTTKDEVYEDTEWGRSNPQMAVMFGKIKVSDLGEILD
                     FIPLFHRCEEYYQ"
     gene            7189..8862
                     /locus_tag="PLL28_02175"
     CDS             7189..8862
                     /locus_tag="PLL28_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68149.1"
                     /translation="MRKFLFTALCAFSAMTLAAQNFNGLSVSTGNMYPDGSNYGVVLQ
                     NELIQVQQLDTTVKYDVVYEFKNNTSTFATVTAALPVNVYFNEFAPGKRSEMLDKLAS
                     ISTFSDIFAVQDKSQDTREQIRNNFQGRKFVRKYVSADNLKAVGIHVDLFRNNTRINI
                     KKIFCEIKFTDATPLYLPKNTEVLSMEIKFIVEMNFTPDEMTSVMAFLTMPAINAGIG
                     TKETYSMYSLGYEKNWSGRLEALYIKNDIFNATPIMPTKFSEYTTYIDGDRNQVLKYE
                     DIMLMDKDKMAFYNINNAASCTTGTSYTEQLFIPSAIKNITASSWVKTDQEYAKRAFK
                     QTPMIAFSDSLHPYQTGNPTVVDALHSSSMKAKLYDQSNLYNFIAAGCKNNTPSITFK
                     ESGNPLYAFDISDHSYEDSTYDGREHLSRQTCWCEGVNGPGVGEYIEFELTQPSHEMH
                     IYNGNQSSKKAFDESSKADIIVVTSLDGHKINPNGDNPSVYRSSIIDLTILNVYELAM
                     PAGKYRITIDAVDKGTVPTTCLSSIGFDFQVMDEWYMRSQSMLRSFFKK"
     gene            complement(8964..10079)
                     /locus_tag="PLL28_02180"
     CDS             complement(8964..10079)
                     /locus_tag="PLL28_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HNF68150.1"
                     /translation="MSKELKFKPIEEWGFNFESPLIMAGPCSVESEEQVMETALQLKD
                     QRFDILRGGIWKPRTRPNSFEGIGSQGLKWLKDAGNAIGKPVATEVANVKHVYESLRM
                     GIDVLWIGARTTVNPFAVQEICDALQGVDIPVLVKNPINPDLELWIGAFERLNRAGIT
                     KLGAIHRGFSTHEKTKYRNIPRWNIPIELKRRFPGLPLICDPSHICGTREYLREVAQT
                     AMDIGFDGIHLESHINPDKALSDAKQQLTPAAFGELINSLQMRQSLEQNPYAENQLEM
                     LRGRIDHLDHYLIEMLSERMDVVREIAEYKREHNLAIVQPSRWTEILEDRVNSGQRKN
                     LTEKFIKELFHAMHKESIHHQTEVMLQPDGNKVEKNA"
     gene            complement(10103..10885)
                     /locus_tag="PLL28_02185"
     CDS             complement(10103..10885)
                     /locus_tag="PLL28_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HNF68151.1"
                     /translation="MRISLLGEPYSFHHVAATQYWGIDTDILFLDDFDEVIKAVQNGL
                     ADHCIIAVENTVAGEVPGNYKRVVNSGLHIEGEINLHLAMHLAAKQVVPVETLHAVIS
                     HPMALKETAAFFLNYPSIKRISASSTSAAVKTVAESDDEGMAAIGNTSAIRFYNLQII
                     VKNIDNLPNNITRFLILSKQYSNPDATSSPVKASVKLLSASPALPVSIGLRRDFDDRI
                     TYVELPEGPLALILEQIDRLRAAAEDVTLLGVYPTGETASGS"
     gene            11087..12160
                     /locus_tag="PLL28_02190"
     CDS             11087..12160
                     /locus_tag="PLL28_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNF68152.1"
                     /translation="MIDKNIRIAATPPGDFYLGEDQYALVREKVFTRSWHYIGDKHQF
                     QETGYLYPVNLLPGCLNEPLILSVQDDVLQLMSNVCTHRGYVLLTEKTKSQVIRCKYH
                     GRCFHPDGAFMSMPEFEEAENFPTRMDDLAKAKVEVWNGLYFGALFPQVDLHALLKPI
                     SDRLSWFPFEKLKFRADVSADYDVKANWALYCDNYLEGFHIPFVHKSLNRVIDYHAYE
                     TILFPGGNLQIGIAKEGELTFNIPPTAEDHGKQIVAYYYWIFPNMMFNFYPWGLSLNI
                     VQPQGKDNTKVSFLIFVADEEKYNTGAGSDLNRVELEDEEVVESVHKGLQSRLYFRGR
                     YSPKMEKGVHQFHQLLETAMHNS"
     gene            12382..12549
                     /locus_tag="PLL28_02195"
     CDS             12382..12549
                     /locus_tag="PLL28_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68153.1"
                     /translation="MRFPELPFGCPRPLLLTAPDSHQVFQIYLVTAFKPIEIGIFLRT
                     AFAQGLCTNGG"
     gene            complement(12632..13963)
                     /locus_tag="PLL28_02200"
     CDS             complement(12632..13963)
                     /locus_tag="PLL28_02200"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68154.1"
                     /translation="MRNLLLLGIIFIHASLSAQCFIGIDSIYCTNGIPDTILPVDTGG
                     YFDGDGISANGIFFPEILDPGSYVISYKNTQYYYVDTTGVFAPLSGDEQYVSLIDDGF
                     SDGIDIGFNFEFFGSLKNYVRIHSNGFITFNFDLAIFPPYANDIPTPGMPTDLIAASW
                     GDYDPTMGGTISWFQSGSAPNRKLVINYRDLPPYGETEGLLTTQVILFETTNIIEIHT
                     TASPDYGKRKTQGIENIDGTAGYVRPGRNNSYWETYNDYVAFIPQFCIDTLYVENPPV
                     TEVLDDEINICMPYAGLLHVSGADTYLWLSDDICTMTPGPDPDSYYLHGIAEGTFTCN
                     IVGFNEAGCSDTSQVVVNIETCQTAITNNVETPFQITPNPANNILTIIIAANEFNGKW
                     SLVNLQGAEMQFGELTGLKTEIDIRELPAGIYFLIFDAGDCSAREQFIISH"
     gene            complement(14011..16407)
                     /locus_tag="PLL28_02205"
     CDS             complement(14011..16407)
                     /locus_tag="PLL28_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNF68155.1"
                     /translation="MRIFPADIPQVLEFEKVLHEVARYCDSSPGADRVARVRPLDKFD
                     EVKKMLLQTREMTHLLQYAQGFPHDKMFDIRPTLPLLAIENYVLSEQQCHEIHRVALL
                     MGSIIRFFNSNQEQYPVLQQFVSGTTYVSAISQSIKAVIDDAGHMRNDASPELVRILK
                     EKDDAMRSLGKVFQSALQKYRSAGFLSEVEESMRNGRRVLGIQSEYKRKIAGIIHDES
                     ETGRTVFMEPQEVVNIQNEIFELEREEKREIYRILKTLTADLAPHQQILDKYQQILGI
                     IDFTRAKGRYALETQSSMPQLEKQPIVCLYDARHPVLLSLHKKQHKQVVPLQAELDSN
                     NRILVISGPNAGGKSVAMKTIGLLQYMTQCGLLIPASSHSRIGIFKQIFCDIGDTQSL
                     EDELSTYSARLMKIRHFLQHADRETLLLIDEFGSGTDPVMGGAIAEAALRMLNEKKIF
                     GVITTHYANLKSFAANAIGLFNGSMLYDEAELKPMYLLETGKPGSSYAFELAKKSALP
                     GTLIEEAKKLISKEHIRFEELLKNVRIEKEHIRLRDKEVSKKEEQLKKKEVELAAAME
                     KTREQREKYNLKKLEKEDQTIREMEATFKNLLQEFKQVQSDPEMDNATKEQSKDTVRK
                     FFQEQRKKTFDHQKKIRPTPIPQWQRGEIKIGGYVKLNSGSEIGVLESVKNNKATVIF
                     NNIRTTVPYADLIGIAKTEAPAITPAKIKVTIDESDFNTELDLRGQPREEAMMNLEQY
                     LDAAMMRKIYQLRILHGKGTGAIREVVQQTLKSHPAVLRFEFANREGGGDGVTLVTLG
                     "
     gene            16492..17409
                     /locus_tag="PLL28_02210"
     CDS             16492..17409
                     /locus_tag="PLL28_02210"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HNF68156.1"
                     /translation="MCDIIDAKQLNFDQNNLILLNCLLAFLMFGIALDLKVEHFKRLI
                     QYPRKAIAGILSQYVLLPFLTYLIVIILQPCPSIALGIFLLGACPGGNMSNFLSSLAK
                     GNVALSVTLTATSTLLAPIMTPLNFAFYGGLYPPTAELMQEISVEPMELFTTIVVILG
                     VPLIIGMLVNHFMPKFTALIRRPVQIVSVIIFLCFIVFAFLKNYDLFMEIIGMIFLLV
                     LLHNGLAMLGGYGLGALFKLDFADKKCLSIETGIHNAALGLIIIFSFFKGLGGMAVVA
                     GWWGIWDLVTGFIIATYWSRMAAKKTLQA"
     gene            17437..18825
                     /locus_tag="PLL28_02215"
     CDS             17437..18825
                     /locus_tag="PLL28_02215"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNF68157.1"
                     /translation="MDKRLLRRLFEKQNPIPFRDTTLHTYTKGYWTVQPFLIGIPYKY
                     YYSKYEVAGWEQVPTDKPVIFAISHRNAFMDSLAFVNCKGTQVWQLARGDAFNNPMMK
                     KLFIFFHMLPLWRERDGVDTKNMNQPTFEACADILYNNGMVGIYPEGNCINEEHIRPL
                     KKGICRIAFLAAEKYQFQPDIHIIPVGVMYSGAEKFKHTQLLNFGKPILLKEYYEQYR
                     SAPAQTINILKDDIEKAMKETATHVPHGTFHHDIVELSKMHARETLLNNNIPVTPLNS
                     FNEEKVISAKLDAYQHSDHEGMKRLVQNLHNYKRIRNQHNLKENTFDKSRSHTFSMVF
                     MALYFLLMFPVYIYGAIINYLPYKFPQQFVEKKIKQKIFWSSAKYVISLFLFPIYYLI
                     LFIITWTVLGSIVSGLIFLFSFPISGNIAYYYYFDFKKWLSALRFKRLPEHEKAALIQ
                     ARDSVYQNLASL"
     gene            complement(18826..20262)
                     /locus_tag="PLL28_02220"
     CDS             complement(18826..20262)
                     /locus_tag="PLL28_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HNF68158.1"
                     /translation="MLTNETFRNQAHQVVDWIADYLENIQQYPVKPSIAPGALRAALP
                     DEAPQSGEAMDDIWKDFLNMIVPGITHWQHPGFMALFPSNASYPGILGEIAVSGLGIN
                     AMMWDTSPSATELEQTVMEWLRKATGLPDGMLGVIHDTASMSTLTAIISARERYAQFQ
                     VNQHGFHGYDKLVMYCTDQTHYSIEKGAKAAGIGAAQVKKIATDDAHAMIATALTKQI
                     AEDKAAGLQPFMVVATLGTTSTLAFDPLEGIGNICVEHKLWLHVDAAYAGSAFFLNEY
                     RHFLQGIDHADSYVFNAHKWLFTNFDCSCYFVKDANHLINTFSANPDYLKRTEESVAM
                     FKDWGIPLGRRFRALKLWFVLRNYGISGIQELLRKHIDIATNFRNQLLSDTQWELMAP
                     PVMNMFCVRYHPQGINDNEALNALNTALLKSINADGNYYLSGTIVKGMYMIRVVMGQT
                     HLEQVHAEGLLALMRSSANSINDFKALR"
     gene            20268..21188
                     /locus_tag="PLL28_02225"
     CDS             20268..21188
                     /locus_tag="PLL28_02225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNF68159.1"
                     /translation="MQYDWFVIINPVSGGKAAPKRWHKIKPLFDAAPFNYEVIFTEYR
                     DHAALIAKNAVQQGFRRFVILGGDGTANDVINGLCQSGISTEMFTVAMIPAGTGNDWV
                     RTIGKPDFAMIVEDLIRHETMKHDIANVTYVNAGKEAMRYFINMAGLGFEGAVAKKIY
                     ERSSKWQGGKMQYQLAILSTLFVYKHTQLEIKVDGNSSEMQFLSAAIGSGKFNGGGMK
                     QLPAAIFNDGILDLTVIATMSKMKMVISLPKLKTGAHIGMQEVQTFKGKIIEMNSNPP
                     VHMDADGEYLGQTPLRFQVASEQINVIKWR"
     gene            complement(21189..22229)
                     /locus_tag="PLL28_02230"
     CDS             complement(21189..22229)
                     /locus_tag="PLL28_02230"
                     /inference="COORDINATES: protein motif:HMM:NF022732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HNF68160.1"
                     /translation="MISGPKALVTIAFAYSLLSACAYMPVKATFTEAEIPAAPDFANP
                     DLWSALPTRHDSADHIPNDNIFPVVDGQADAAVDVFFIYPTQFFSRSEWNASLDDKKL
                     NATIDARAITHQASVFNGSCRVYVPRYRQATFNAYFSLTNPDAKKAFELAYSDIKAAF
                     EYYLEHYNNGRPIIIAGHSQGTTHAKWLLRDYFDGKPLVDKLVAAYLIGMPVYENDFT
                     QIAPCDSAGQTGCYVSWRSYLEGSEQKPKLIAADRDKIVVHNPLTFSRTTDFISSDHN
                     IGGLDRDANTLIPNACSAQIHEDIVWVSRPDIRGKAFIPENLHPADYNLYWLTIRNNV
                     QMQVNNYLQTHK"
     gene            complement(22259..22912)
                     /locus_tag="PLL28_02235"
     CDS             complement(22259..22912)
                     /locus_tag="PLL28_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNF68161.1"
                     /translation="MSSTYHEIKTFLDARKVQLIAVSKTRPIAQLMPLYEAGQRDFGE
                     NRVQEMREKHELMPADIRWHLIGHLQRNKVKYIAPWVHMIHSVDSADLLKEIDRQAVT
                     CNRVIPCLLQIYVATEETKFGLDEPELMALLSSEAFHAMHNIQICGLMGMATNTENQE
                     QIRKEFATLHAIFERTKARFFAQDNNFKELSMGMSSDYTIAVEQCSTMVRIGSLLFN"
     gene            22948..23340
                     /locus_tag="PLL28_02240"
     CDS             22948..23340
                     /locus_tag="PLL28_02240"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HNF68162.1"
                     /translation="MRLTFLICSALLMMTFACKPKDPFVVNDQMLNSVPDSVIVVDSM
                     INILTDIQLAEAYVNEMRSDSIPKEDRLKQYYSEIFSMHHIDGTSYKRSYDYYVGNPI
                     LMNHIYVKVTEKLNLMESEHATVKKNVK"
     gene            23337..24029
                     /locus_tag="PLL28_02245"
     CDS             23337..24029
                     /locus_tag="PLL28_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HNF68163.1"
                     /translation="MNKVFKNADEAIADIFNGAILMLGGFGLCGIPENCISALVRKKV
                     NGLTCISNNAGVDDFGIGLMLQQRQVKKMIASYVGENAEFERQLLSGELEVELIPQGT
                     LATRCMASGYGMPAIFTPAGEGTEVADGKETRVFNGKTYLLEYAFDADYAIVKAWKGD
                     TMGNLVFRGTARNFNPLMAMAGKITIAEVEELVPAGQLDPNNIHTPGIYVHRIFQGAD
                     YEKRIEQRTISK"
     gene            24053..24706
                     /locus_tag="PLL28_02250"
     CDS             24053..24706
                     /locus_tag="PLL28_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HNF68164.1"
                     /translation="MLDKNQIAKRIAQELKDGYYVNLGIGIPTLVANYIPSGMTVTLQ
                     SENGLLGMGPFPKDEDVDADIINAGKQTITTLPGSAFFDSAMSFGMIRAGKVDLTVLG
                     AMEVADNGDIANWKIPGKMVKGMGGAMDLVASAKNIIVAMQHVNKEGQSKLLKSCTLP
                     ITGRGVVKKIVTELGVFDVTPQGFKLLERAPGVSVEEIQSKTEGRLVVEGDIPEMIL"
     gene            24724..25557
                     /locus_tag="PLL28_02255"
     CDS             24724..25557
                     /locus_tag="PLL28_02255"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNF68165.1"
                     /translation="MVFYNHNYIQDDALHLLPSNRAFKYGDGIFESCLLNEGALPLMA
                     YHTTRLMQGMQCLHMDALPHLSEKWLGDVIKELALQHGYQSARAKISVWRGGEGLYLP
                     KSDSPELLIEVFPLHSTPFSEAPQQYVIDIYDGLQKAIHPVSACKTMNALPYILAAKF
                     AASHKWNDVLILNTQNEIADAISSNVWLIKDHMLITTPALNGGVEGTMQAWLLDHVEE
                     LGFSASRKSLTISDVESADAVFLTNAIKGIIPVTRFREKAFTNNLPLALLRDVKTLLF
                     A"
     gene            25550..26866
                     /locus_tag="PLL28_02260"
     CDS             25550..26866
                     /locus_tag="PLL28_02260"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNF68166.1"
                     /translation="MPEINNIPVAIHWFRRDLRLTDNVALSLALRKHERVIPLFIFDK
                     EILDQLEPDDRRVTFIHDVLLQMDATLKTWGSSLLVKYGAPADVWRSLIAEYHIEAVY
                     TNHDHDPYALLRDGNIGALLADHHIPFTTCKDQVIFEKDDVLKDNGTPYTVYTPYSVK
                     WKSLFKEEYLNEHQIFPFRKHFHQFQATPIPSLAMIGFDGERADIPKMLPDEVIIRNY
                     HNTRDLPAINGTSRLSVHLRFGTISIRSLMRETRHLSEKYFNELIWREFYQMILAQFP
                     HVITNSFRPEYDRIQWRNNEREFDAWCQGKTGFPIVDAGMRELLATGHMHNRVRMIVA
                     SFLTKDLLIDWRWGEAWFAKHLLDFDLASNNGGWQWAAGCGVDAAPYFRIFNPYEQAK
                     KFDPEGKYIRKWIAEIDELTYPQPIVDHAFARKRTLDAYKIALSNQ"
     gene            complement(26860..27315)
                     /locus_tag="PLL28_02265"
     CDS             complement(26860..27315)
                     /locus_tag="PLL28_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68167.1"
                     /translation="MACNHTNSSTSPEDFANTLSIQHIDAENAIMRQNIERICKDSNC
                     AYFSFYGKSCKNLIDTISTLYSKYQNIDGKVHFSVFMNEKRSCLQIFGNQSETHGLYS
                     EHITHAYLADTIISGDAAILQLELLEQDILERYNPIKRVISSNCGLMIY"
     gene            27498..29165
                     /locus_tag="PLL28_02270"
     CDS             27498..29165
                     /locus_tag="PLL28_02270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HNF68168.1"
                     /translation="MKSLCTALLICLSLPTWAQKSIVVNTDIEKATVFLNGAQLFHTA
                     SVHIPSGPSEIVLQGFSNSLDQNSIQGGGKGDFTILDIQYRLFYPEPVDVTPELSAID
                     KKIKIKQDSIVEVDYDLRAIAAKQEALNVQKMMLMNNALLHNTNNDSLELLQHSIAYY
                     ESKLSEVLDKLLELDKQSYVLNNRRAAITARISELYAYRSQQAAGSNPNAMIPQVVMQ
                     VYADAAVDAKMEVNYITYNAGWYATYDIRANDIAKPVELAYKANVWQNSGIEWKNVKL
                     TCSTGNPMQGNTLPELTAWYLGYYQYYYDRDVQTLSGATAPAAATKSLEEVAGYDDFK
                     DESLQKSTNASNFTTQNATVANVEFEINLKYTVPSDGKGHVIALQSEQLKTKYNYLII
                     PKLEQSAFLIARITDWEKINLLPGNANIYFNNTYVGKTYLNPLTLSDTLSVSMGRDRS
                     IEVKRELLADKSTDKILNAKAHKTMSYTIDVRNTKAIGIEIILKDQVPLSNDDDVKVE
                     VLEQSAAQLNEDTGILTWRETLASKQKKTWTFGFEVSYPKDEPLSLQ"
     gene            29412..30053
                     /locus_tag="PLL28_02275"
     CDS             29412..30053
                     /locus_tag="PLL28_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68169.1"
                     /translation="MYTLPKFTKSLAMVAAAILLTVSTSLYAGNGNYRNSPYGRDPNP
                     VRVYYFGYDLTNPGFGIGTEVNLSWTKMEKSGCKGSRISDRQFMMIPNLGMFTNEAGS
                     QSIFGNLEFAYAVTYRHGLTLEVFGAGGYAQMLSELTNDQADNSKITNGTASETAYSG
                     FMPSAGVGIGYDFQKLNGKDFPLELNFRGLATSTNIGESIITPAFQTGLIYSF"
     gene            30121..>31911
                     /locus_tag="PLL28_02280"
     CDS             30121..>31911
                     /locus_tag="PLL28_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68170.1"
                     /translation="MKKYTLLVLTLAISMGLKAQYPLVEIYDIQYKDAAALAAEDDLS
                     AYDGDTVRIQGIVTFNPCDYALSSTGSRMGTWLQDEDGGPFSGIHVLIDFPAIGYTDL
                     ESLNDATLFVDNFQVGNIVECTGIVNSFEGNTQFLLLPIETSIVGFTTLPTPVPAEVS
                     ELEVSDGAGGQVINTVDGEQYEGRYVEFTNVYVTDVTPSGLRWFWYLQDADGNKIQIR
                     DVSGHFRNDTYDDECNMWAGGAAGETNTPDAYTPPAIGTNLAYVRGVILEFTAATQYG
                     IAPLTLEDIGPSLLSPPIITDITRTPVVATPADEVTISCSITDLDGSVASADLYYSYG
                     YGSTDWNLATMSNTGGDSWAGAIPGPGIDSTYVNYYITAFDDDGNSISNPTAAAPNTY
                     IVYADGINSIVQIQNTPYTSGASVWANDSIVDMQIEAIVTSTTQTYDLGMVAVQEGEG
                     AYEGIFIKSVPGDGTDVLNRGDLIRITSGKVIEEFGVTKLTNITYTKLSEMNDLPAFH
                     TGLNPLDVDAKLYAATEPWEGMLVRFDNAFVTSNNADAATGGAFGEWRVNLSNTPEVG
                     MRCDDYSYELPFEFGTDSLAMGQSLSYIQGL"
BASE COUNT         9119 a         6637 c         6639 g         9516 t
ORIGIN      
        1 tacttgatac ttgatacttg atacttgata cttgatacca ctcaccactc accactcacc
       61 actaaatcaa tgcacattcg gtatttcccc tttattaaat tcaaactgat catctttttc
      121 tttgtattgt aatacaagat ccagagcttc ggcgatgacg tcggagcaga tggtgatatg
      181 tgatcccggt ttggcgtttt caatcacata tttaatactt tccttttcag gatttattct
      241 aatcacatcc agtccgggtt ttatttcaag tatgccgtcc attaatagat tcataatttc
      301 ttcatcggtt cttcccctta gatttttatc ctgacgaata actatctcat caaaggtgcg
      361 cgcagcaatg cgtccgagtt cacgaatatc ttcatctcgt ctgtcgccgg cggctgcaat
      421 taagcccact ttaattggtg catttacttt gctgagataa ttgctgatag cctggaaccc
      481 atcaggattg tgtgcaaaat ctaccaacac ttcaaaattt ctgaatttaa aaatattcat
      541 acgtccggga gtggttgcag gacccggaat aaatgtttga agtgcaagct taatttcttc
      601 cactttaaat ccttgcagat aagcacataa agttgcaggg aggatattct gaatcatgaa
      661 ggtagccttg ccatcgaacg tgagtggaat attcacaact ttttctacac gcagtttcca
      721 atttcctttc aaaattgtaa tccatccatt ttccagtacc gcacaaatgc ctccgcgttc
      781 gcaatgttcc atgatgcgcg aattattttc atccatactg aacaatgcga ttttacattc
      841 cagattttta cgcatattga aaacgagatc gtcatcagca tttaaaattg cgtatccgtt
      901 tttatgaact gtttccggta atacagattt tacctttgcc atttcttcaa cggtatcaat
      961 atctttcaat ccaaggtgat ctgcagttac gttggtgaca atagcataat cacatttatc
     1021 aaatcccaat cccgctttta ataatccgcc acgtgcacat tccaacaccg catagtcaac
     1081 tgttggatcc attaatacaa atttagtact tacaggaccg gtgcaatcgc ctttttctaa
     1141 catgcgattt tggatataga taccatcgct cgttgtgaag ccaaccttat gtcccatcgt
     1201 ttttgcaata tgcgcacaaa gccgggtggt tgtagtttta ccatttgttc ccgtgatggc
     1261 tacaattggt atgcgacctt gcgtgccggc agggaaaagc atatcaacaa cgggttctgc
     1321 aacgtttctg ccaatacctt cgcttggagc aaggtgcatg cggaatccgg gtgctgcatt
     1381 tacttctaaa acaacaccgc gattttcagt cattggtaca gaaatatccg gactcataat
     1441 atctatacca caaatatcga gaccgatagt tcttgcaata cgttctgccg taaatacatt
     1501 gtaaggatgt acaatatcag tcacatctgt ggcagttcca cctgtgctga gatttgctgt
     1561 tgattttaaa aatatttctt cgccgggtga agggatactt tcctccgtca tccccaattt
     1621 ttcaagcaaa cgaagtgtat gatcatcaat tttaatgctg gttaatacgt tttcatgacc
     1681 gtagcctcgg cgcggatcgg catttaccat atctaccaat tgtttgacgg tatgcacgcc
     1741 atcaccaata attgcagccg gtttgcgaag tgcggcagaa acaaactggt agttgattac
     1801 gagtaaacga taatcatatc ctacaatata ctgttcaacg atagcccatc ttgaaacctc
     1861 tttcgatttt tttaaggcag caactgcttc ctcccagttt ttaatattca tggtagaccc
     1921 gcgtccatga ttaccatcga taggtttaat cacaatcggg tagcctattc ttttaatgat
     1981 tgcttccaga tcatcctcat cataggcact tccacctttt gctaccggaa tatttaattg
     2041 ttgtaataga ttttttgttt cttctttatc acaagcaatt tcaacagcta tattagaggt
     2101 agtactggca acggtagcct gtatacgtcg ttgatttttg ccccatccca attggacaag
     2161 tgatcgtcta ttaagtctta tatagggtat atcgcgcttg atagcctctt cgacgatact
     2221 tccggtggat ggtccaaggc gttcccgctc ccggatctct ttcattttca tgatatgcgg
     2281 agcgatgtcg taaaattctc cgcgaacgag agattcgcag agttcgacag caatttttcc
     2341 tgcatatatg cctgtttgtt cttcaaggta ggagtacact acattataaa caccctttgt
     2401 tgaggtgctt cgtgttcgac cgaagcctgt gtccaaaccg gctagcgttt gcaattcaag
     2461 agcaatatgt tccaccacat ggcccatcca cgtgccttcg tcgacgcgtt tgaaaaagcc
     2521 tcctgcatgg tcttcagagc atcggtgctc atacaaacta ggtatgatct gttccagacg
     2581 ctcacggaac ccatggattt tattggtagg catttcctcc aactcctcca ggtctagctt
     2641 catcacgatc agtttatggc ggcggatgct ccaatagttc gggcccttca ttacggcggt
     2701 ggaaaggatc ttcatgtgtt tgctttgcgg ctaagttaaa aaaaaggcag atatgatgct
     2761 tcgaattttt taatgtagcc acggagactt gttgccgtta gatatcctaa ataattgtaa
     2821 cgcgatatcg atacttaaat tattgtggat atgtcgatgc attgcaatag cctgtaacaa
     2881 atatatttgc ccgatcattt taccattgca tgatacctaa aggcaggctt atagccgttg
     2941 gaggcgcaga gcaccgagat catattgaac aggataagct ggaagtttta aggcgaattt
     3001 tacaggaaat gaggggcccg gatactgtgc tggaaatcat tcctactgcc tccggcatcc
     3061 caaaacagat tgccagagaa tatactgatg cttttggcac actgggttgc aaggctacgc
     3121 gtatcatgaa tatccgcaag caaaaagatg cgcagaaaaa agaatttcta gaccgtttgc
     3181 gctcctgcga tggtattatg ttcagcggag gaaatcaaac attgcttagt gaaacatttc
     3241 tgggcacaga atttctggat attttaaagt cacgctacat caatgaaccc ggatttgtca
     3301 tcgcaggaac cagcgccgga gcaatggctc agagtggcag catgataaat gggggagccc
     3361 ctgcagaagc attaatgcgc ggaaaagcgc ttatgattga aggtcttgga tttaccaata
     3421 attccattat agattcccat tttgtaaacc gaggtcgatt cggtcgcttg atggtagctg
     3481 ttgctgaaca tcctcagctt accggaattg gcattagtga agatactgcc gtaatcatca
     3541 aagaagaccg ctatcttgaa atcatcggaa caggactggt tgtcattatg gacggaactg
     3601 aactgcgatt aaataatata gcaaataata aaggtgaaat ggtaaatcta gaacgcatga
     3661 tctttcacct cctttcaaaa ggcaggcatt atgatatgca ggaaagaaag gtgattacaa
     3721 attaataaat acccgtgaag taatctgctt cggatttcat ccatcctctg tacatgcccg
     3781 gtgtattaaa gggcaaacaa atatttcctt tggcatcgat ggcaattaat ccgccttcag
     3841 cttcaatggg ctttaatttg tcgtgaacga tatgatttgc agcttcctct aatcccattc
     3901 ctttataagt catcaggcaa tggagatcat atgctacaac ttgtcgaata aaaaattcgc
     3961 catgaccggt gcaactcacc gctacagaag cattgttggc ataggttcct gagcctatca
     4021 atggtgaatc gccaacgcgg ccatataatt tgttggtcat gcctcctgta gatgttgctg
     4081 cagccaaatt tccttttgca tcaatcgcaa cagcgcctac agttccaaat tttttatcgt
     4141 cgacatgatc tagaacagtt atctttttct tcaatgcctt ttgccattga tcatacctca
     4201 attgagtgaa gaaataatcg tcgtcttcaa acactatatt atgcaatctg gcaaattctt
     4261 cggctccatc gttgagtaat aatacatgtt ccgaattatc cattacacat ctggctaagg
     4321 tgaccggatt tttgatattg ctaacgcctg caacagcgcc acaatcgagt gtttcgccat
     4381 tcataatgga tgcatccatt tcatgctgtc ccttgttggt gaataccgat ccttttccgg
     4441 cattgaacat gggggcattt tcaagcgctc ttaccgcagc ttccacgcca tcaacagcag
     4501 tgccaccttt ggccatggca ctgtatcctg cctgtaacgc caattgaagc gaatcatggc
     4561 acgcagcctc tatttcaggg gtcatgagtc tctttggcat ggtgcctgct ccaccatgta
     4621 ttgcgatgcg aattggtgca ttcatcacat aaaggtagaa tttttgattt agcagaaaag
     4681 agtggtatgc acacctgttg aaccatgacc tccgttaatt gttgttaatt agggaacgca
     4741 gcgaccatct gaattggtta tgtttgcgta agtgtgataa agctatcaat atgaaaggaa
     4801 ctaaaacaat cacagcagca ctgcttagcc ttgtattagg catcaccgga tgtggtgcga
     4861 aacaggcaac cagcgacaat caacaaattc aagataacaa gcaatcaaac gatctggcaa
     4921 tgcgtaaaac ttatacagat gaagagatcc gtaacaacgg atgggaaaaa gcaacatttg
     4981 gtggaggatg cttctggtgc acagaggcga ttttcgaaga agtgaaaggg gttaaatcgg
     5041 tagtttcagg atatgcgggc gggaaaatgg aaaaacccac atacgaagcc gtttgcaccg
     5101 atactaccgg tcatgcagag tgtgtgcaag tagttttcga ccctaaagag tgtgcgtttg
     5161 caacaatatt ggatgtacat atgaaaacac atgacccgac aacattaaat cgtcagggaa
     5221 atgatgtggg aattcaatat cgttcagtag tattttatga aaatgatgca caaaaaactg
     5281 ctactgaaga ttacatcaac aaattaaatg cttccgggta ctacagcgat aaaattgtta
     5341 ccgaagtggt gccattaact acattctggc ctgcggaaac ctatcatcag gattattttg
     5401 ccaaaaatcc cgaccaaccg tattgtgttt atgtggtagc aaaaaaggtt gataaatttg
     5461 aacacctttt ccccgaatta atagagaaat aaatcgtatt taatatagtg taagtatcat
     5521 ttttggtgca gactcgttac ttaaatccag ctgcaaagaa aatgatgctg catgcgtact
     5581 attgctgaat ccattaatgg taaaccactc agttttaacc gggccatctg tgcttgaact
     5641 atggttgaaa ttactgttga gtaagcattc tgaaaaggtt gtggtaaggc tgtcaaattg
     5701 agatattgca agggcttcag atgccaattc gctatatact tcagtatatt tacctcgaat
     5761 cagtcgacca cttgttccat tgaaaaatgt gttttcaaaa gtggaaacct cttcatatgt
     5821 ctcctcattt aataccgttg ttattggaaa cattttccag tcggcattcc attgattgag
     5881 cagataaact aatttcctgc aatcaggcat ttgtttatca tagaataact gatcgcccga
     5941 gaattgcttg actacgtatc tgaatgtacc tgtttgatat gactccttac ttgttaacag
     6001 tataaaaaca gtagtatctg ctaaggttgt aaagttgata taacttccga aaacatcaga
     6061 tttgatttgc tcaatccatc cttgcacaaa accggtcgta tcttcattga ttagcgtaac
     6121 aaatggtata aaactgctgc tttccatata gaccagcaag ccatctcccg ctttcaaatt
     6181 gattgaaaaa tatttgtaat agttgccttc ctgaagtttg tcatcagaag tggtgagcga
     6241 ggaggtatca tttgcctcca caggaccaag tgtgatgctt tgcgcgttaa caaaactaga
     6301 cgtaatgcaa aggatcaaat gcagtatgat aattctcatt atgaaggttc tttattcagt
     6361 ttccatccaa tcgtcgaggt cgcgacgttc ttttttagtt ggccttccgg tgcctttttc
     6421 gcggaatgcg gtgggcataa actccaaggt tgtaagtttc attttctcct cttcaggggt
     6481 cacatcttcc atataatcct tcaccagcgg agcgccaaca cgtctttcca ggggtgccaa
     6541 aaccttgtat tgatacttta cgactccttt tggaatagtt agtatcagac ccggaaatac
     6601 ttcaaatgaa ggcttgactg agtcgccatt gaccttcact ttgccatttt tgacagcttc
     6661 ggttgccagg ctgcgggttt tgtatagtcg aacagcccat aaccacttgt caattcgcgt
     6721 ttttgttaca tgctcaggca cgatacttgt aatgttaagc cgtaaaaata agctacattc
     6781 ctgtatctta gcggctataa gattttacat gcaaaaacct atttccgcac gcgagattgt
     6841 gctgtcactt aaagaccgat tccgccctga gaaggcagaa gcgggatatg agactgtttt
     6901 tcatttggac atatcgggcg atcgtggtgg acaattcacg gtgaccattg ccaacggaac
     6961 cattaatatt gaggacggat ttcatggcaa tcccaagtgt attgtgacaa ccaaagatga
     7021 ggtatatgag gatacagagt gggggagatc gaatcctcaa atggccgtta tgtttggtaa
     7081 aattaaggtg agcgacctgg gtgagatact cgatttcata cccttgttcc accgttgcga
     7141 ggagtattat cagtaaccag tagaataaaa aagcgaaaat tgacagctat gcgaaaattt
     7201 ctttttacag cgctctgtgc cttttctgca atgactcttg cagcacagaa cttcaatggg
     7261 ctgagcgttt caaccggaaa tatgtatccc gacgggtcaa attacggtgt agtgcttcaa
     7321 aatgaactta tccaggttca gcaattagat accactgtga aatatgatgt ggtgtatgaa
     7381 ttcaaaaaca acacatcgac atttgctacg gttactgcag cgcttccggt aaatgtctat
     7441 ttcaatgaat ttgctccggg caagcgtagt gagatgctgg ataaactggc gtcgatttct
     7501 acgtttagcg acatatttgc tgttcaggac aaatctcagg atacccgcga acagatccgc
     7561 aataattttc aaggacgcaa gtttgtaagg aaatatgtga gtgccgacaa tctgaaagca
     7621 gtaggcatcc atgtcgacct tttcagaaat aatacccgta tcaacatcaa aaaaatattt
     7681 tgtgaaataa aattcacgga tgcaacacca ctttaccttc caaagaatac agaggttttg
     7741 tctatggaga taaagtttat tgttgaaatg aattttacac cggatgagat gactagtgtt
     7801 atggcatttc ttaccatgcc ggcaattaat gcgggtatcg gcacaaaaga aacctattca
     7861 atgtattcat taggttacga aaaaaattgg tctggtcgcc tcgaagcatt gtatattaaa
     7921 aatgatattt tcaatgcaac accaattatg ccgacaaaat tttcggagta taccacctat
     7981 atagatggag atcgcaatca ggtgttgaag tatgaagata tcatgctgat ggataaggac
     8041 aagatggcat tttataatat aaacaatgca gcaagttgca ccacgggtac ttcatatact
     8101 gaacagctat tcattccatc tgccataaaa aatattacag ccagcagttg ggtgaaaacg
     8161 gatcaggaat atgctaagcg cgcatttaag caaacgccga tgatcgcttt ttcagattca
     8221 ttgcatccat accaaaccgg aaatcctaca gtagttgatg cattacacag cagctcaatg
     8281 aaggcgaaat tatacgatca gtctaacctc tataatttca tagccgccgg ctgcaagaac
     8341 aatacacctt caatcacatt caaagagtcg ggaaatccat tatatgcttt cgatatatct
     8401 gatcattctt atgaagatag tacctatgat ggtcgcgagc acctatcacg tcagacctgt
     8461 tggtgtgaag gcgtaaatgg ccccggagtt ggcgaataca ttgagtttga gcttactcag
     8521 ccatcacacg aaatgcacat ttataacggc aatcaatcgt cgaaaaaagc attcgatgaa
     8581 agttcaaaag ctgatataat agtagtaacc agtctcgatg gacataaaat aaatccgaac
     8641 ggtgataatc cttcggttta tcgcagttca atcattgacc tcaccatcct caatgtgtat
     8701 gaattggcaa tgccggcagg aaaatatcgc attactatag acgctgtcga taaaggtacg
     8761 gtgcctacaa cctgcttatc atctatcggc ttcgactttc aagttatgga tgaatggtac
     8821 atgcgttcgc aaagtatgct gcgtagcttc ttcaagaaat aataacgctt aaacataaaa
     8881 acccgatgcc acaacatcgg gtttttttat gcataaaaaa tcccggcatt gctgtcggga
     8941 ttccataatc tgtttacttg ggtttatgcg tttttttcca ctttgttgcc atcaggttga
     9001 agcattactt ctgtttgatg atgtatggat tctttatgca ttgcgtggaa taattctttg
     9061 atgaattttt ctgtcaaatt tttgcgttga ccggaattaa cgcgatcttc caatatttct
     9121 gtccagcgac taggttgcac gatggcaaga ttgtgttcac gtttatattc agcaatttca
     9181 cggactacat ccatacgttc acttaacatc tcaatcaaat agtgatcaag atgatcaatg
     9241 cggccacgga gcatttccaa ctgattttca gcatatggat tttgctcgag cgattgacgc
     9301 atttgcagtg aattaatgag ctcaccaaat gcagcaggtg ttaattgttg tttggcatcg
     9361 ctgagtgctt tatcgggatt gatatgcgat tcaaggtgaa tgccatcgaa accaatatcc
     9421 attgctgttt gggctacctc acgcaaatat tctctggtgc cacaaatatg gcttgggtca
     9481 caaattagag gtaatcccgg aaaacgacgt ttcagttcaa tcggaatgtt ccacctggga
     9541 atattgcgat acttcgtttt ttcatgagta gaaaagccgc ggtgtatggc tcctaatttg
     9601 gtaattccgg ctctgtttag tcgctcaaat gcgcctatcc ataattcaag atcagggtta
     9661 ataggatttt ttaccaatac aggaatatcg acaccctgca gagcatcaca gatttcttgt
     9721 acagcaaatg ggttcactgt ggtgcgtgca ccgatccaaa gcacatcaat acccatgcgc
     9781 aacgattcat aaacgtgctt cacatttgca acctctgttg ctaccggctt tcctatggca
     9841 ttaccggcat cttttagcca tttcagccct tgactgccaa tgccttcaaa tgaatttggt
     9901 cttgtacgag gcttccagat acctccacgg agtatatcaa aacgctggtc tttcaattgg
     9961 agagcggtct ccattacctg ttcctcactt tccacgctgc aaggacctgc catgatcagc
    10021 ggtgattcga aattgaaacc ccattcttcg atgggtttaa acttaagttc tttgctcatg
    10081 cttgtaatat tttgtgttaa ctctaggatc cacttgctgt ttcgccggta ggatataccc
    10141 caagtaaggt tacatcctct gcggcagctc tcaatcggtc aatctgctcc aaaatcaatg
    10201 ccaacggacc ttccgggagc tcaacatacg taatgcggtc gtcaaaatcc cgcctcaaac
    10261 caattgaaac cggtaatgct ggacttgccg acagtagctt tacagacgct tttacaggtg
    10321 aggaggttgc atccggattg ctatattgct tagacaaaat taaaaatctt gtgatattat
    10381 ttggcaaatt gtcaatattc ttaacaatta tttgaagatt ataaaatctg atggcagaag
    10441 tattcccaat ggcagccatg ccttcatcgt cagattcggc cactgtttta actgccgctg
    10501 aggtggaaga agccgatatg cgttttatcg aaggatagtt aagaaaaaaa gctgcggtct
    10561 ccttcaatgc catgggatgg gaaatgactg cgtgtaatgt ttccacagga actacctgtt
    10621 tggcagctaa atgcattgcc agatgcaggt tgatttcacc ttcaatatgt agtccgctgt
    10681 tcaccacccg cttataattt ccgggtactt caccggctac cgtattctca acggctatta
    10741 tgcaatggtc ggctagcccg ttttggacag ccttgataac ctcatcaaaa tcatcaagga
    10801 ataatatatc cgtatcgatt ccccaatatt gcgtggcggc aacgtggtga aaagaatacg
    10861 gctctccgag tagactgatg cgcatgacgc aaaattactt aaaacaggtc ttaatcgctt
    10921 ctccgggctt gaaatttgca catactttaa cgatgatagt taagcggata ccgataaagc
    10981 agagcactat tttcaattga ttttgtaaat tttaattgcg cggcatcacc ttgtttttca
    11041 acaatatcag caaggaaggc gaatttgccg ggattaattt tgtaagatga tagataaaaa
    11101 tatccgcatc gctgcaaccc ctcccggtga tttttacctc ggcgaggatc aatatgcgct
    11161 tgtcagggaa aaagtattca cgcgcagttg gcattatatc ggcgataaac atcaatttca
    11221 ggaaacagga tatctatatc ccgtaaacct gttacccggt tgtttgaatg agccattgat
    11281 attgagtgtg caggatgatg ttttacagtt gatgtcgaat gtgtgtactc atcgcggata
    11341 tgttttattg acagagaaaa caaaatcgca ggtcatccgg tgcaaatatc atggcaggtg
    11401 tttccatcct gacggagcat ttatgagtat gcctgaattt gaggaagcgg agaattttcc
    11461 aacgcgcatg gatgacctgg caaaagctaa ggtagaagtg tggaatggtc tgtatttcgg
    11521 agccttattt ccgcaagtcg atttgcatgc attgcttaaa ccaatttccg accgcctctc
    11581 atggtttcca tttgaaaagt tgaaattccg tgcagatgta tcggccgact atgatgttaa
    11641 agctaactgg gcattgtatt gtgataatta tctggaggga tttcatattc ctttcgtgca
    11701 taaatcgttg aacagggtaa ttgattatca tgcatatgaa acgatattat tcccgggagg
    11761 taatttacag ataggaattg caaaagaagg agaacttacc tttaatattc caccaactgc
    11821 cgaagatcat ggcaaacaaa ttgtggccta ctattattgg atattcccga atatgatgtt
    11881 caatttttat ccatggggat tatcgctcaa catcgtgcaa cctcagggca aggacaatac
    11941 aaaagtgtcg tttttaattt ttgttgccga tgaagaaaaa tacaacaccg gtgcaggaag
    12001 cgatctcaat agggtagaac tggaagatga agaagtagtt gaaagtgtgc acaaaggttt
    12061 gcaatcgcga ttgtatttca gaggtcgtta ttcgccgaaa atggaaaaag gtgtgcatca
    12121 atttcatcaa ttactggaaa cagcaatgca caattcgtaa agtcttttaa tataaaaacc
    12181 tcgcatgacc ggcgaggttt tttttcatcg acgaaattag cgctacacga attccgtcaa
    12241 ttatcttacg aagaaagcgg aatcaacttt ttctcgccga ctccgctttc gcatcatcaa
    12301 taaccgtagt tactgctgat acatctgatt tcgcttccgg gtgtctcact tgcgtggcac
    12361 cttatagctt tccttttcag gatgcgcttt ccggaacttc ctttcggttg tcctcgtccg
    12421 ctactgctaa cggcaccgga tagtcatcag gtattccaaa tatatcttgt aacagcattc
    12481 aagccaatag agattggcat atttttacgc acagcatttg cacagggttt atgcacaaat
    12541 ggtggataag ctaaaagcct tgaaatgcag tcagaatagg gattttggaa ggagtggtca
    12601 gcgtgaatgc acaataaatg cacaaaaaat gttaatgcga aataatgaac tgttccctcg
    12661 ccgaacaatc gcctgcgtca aatatcagaa agtagattcc tgcaggcaat tcgcgaatat
    12721 cgatttcagt tttcaatccg gttaattccc caaattgcat ttcagcacct tgtaaattaa
    12781 ccagcgacca tttgccgtta aattcatttg ctgctataat aattgttaag atattatttg
    12841 caggatttgg cgtaatctga aatggtgttt cgacattatt tgtaatagct gtctgacatg
    12901 tttcaatatt gacaactact tgcgatgtat ctgaacatcc tgcttcattg aacccgacaa
    12961 tattacaagt aaaggtgcct tcagcaatac cgtgtaaata atatgaatcg ggatcaggac
    13021 cgggggtcat cgtgcatata tcatcagaca gccataagta agtatcagca ccgctaacat
    13081 gtaataaacc cgcgtatggc atgcaaatat tgatttcgtc atctaaaact tcggttaccg
    13141 gaggattttc cacatatagc gtatcaatac agaactgtgg aataaatgca acataatcgt
    13201 tgtacgtttc ccagtaacta ttgtttctgc cgggtctgac ataccctgct gttccgtcga
    13261 tattttctat gccttgtgtt ttgcgtttgc catagtcggg actcgcagtg gtatgtattt
    13321 cgataatatt ggttgtttca aaaagaatta cttgcgttgt aagcagtccc tccgtttcgc
    13381 catagggagg taggtcgcga taattaatca caagtttacg attaggagcg gagcctgact
    13441 gaaaccagga aattgtgccg cccattgtag ggtcgtaatc gccccacgaa gcagctatca
    13501 gatctgttgg cattcctgga gtcgggatat catttgcata gggtgggaaa atggcaaggt
    13561 cgaaattgaa agtgatgaaa ccattgctgt gaatccggac atagtttttc aggcttccaa
    13621 aaaattcaaa attaaatcca atatcaatcc catcactaaa tccatcatct attaatgaaa
    13681 catattgttc atcaccacta agcggtgcaa aaacgcctgt agtatcaaca tagtaatact
    13741 gagtattctt ataactgatt acataagaac cgggatccag tatttcagga aagaaaattc
    13801 catttgcact aataccatcc ccatcaaaat atcctccggt atcaacaggt aaaatagtat
    13861 ccggaatgcc gttggtgcaa tatatgctgt ctatacctat gaagcattgt gcggataaac
    13921 ttgcatgtat aaaaatgata cctaagagga gaagattgcg catatttcag actttacatc
    13981 tgaaaattac gacattttaa tgagtgtcgt ttaccctaag gtaacgagtg tcacaccgtc
    14041 gccgccgcct tctctgtttg caaattcgaa acgcaacacg gcaggatgcg atttcaatgt
    14101 ttgctgcaca acctctctga tggcgcctgt gcctttgcca tgcaatattc ttaactgata
    14161 aatcttgcgc atcatggcag catccagata ctgttcgaga ttcatcattg cctcttcgcg
    14221 tggttgtccg cgtaagtcca attccgtatt gaaatcgctt tcatcaatgg taaccttaat
    14281 ttttgcaggc gtaatagcag gagcttctgt ttttgcaatg ccaattaaat cggcataagg
    14341 aactgttgtg cggatattgt tgaatataac ggtagcctta ttatttttta cagattccaa
    14401 tactccgatt tcacttcccg aattcaattt cacatacccg ccgatcttta tttcaccgcg
    14461 ctgccattgc ggaattggag tagggcgtat ttttttctga tgatcaaatg tctttttacg
    14521 ttgctcctga aagaatttac gcaccgtatc tttggattgt tctttggttg cattatccat
    14581 ttcaggatct gattgcactt gcttaaactc ttgtaacaag tttttaaacg tggcttccat
    14641 ttccctaatg gtttgatcct ctttctccag ttttttaaga ttatattttt cacgttgctc
    14701 gcgcgttttt tccatcgcgg cagccagttc aacttccttc ttttttaatt gctcctcttt
    14761 ttttgaaacc tccttgtcac gtaatctgat atgttctttt tctatacgta catttttcag
    14821 taactcttcg aatcgaatat gttctttact gatcagtttc tttgcctcct ctatcaaagt
    14881 cccgggtagg gcagatttct ttgccaattc aaaagcgtag ctactgccgg gtttacctgt
    14941 ttcaagcaag tacatgggtt ttaattctgc ctcatcatac aacatactgc cattaaacaa
    15001 gccgatggca tttgcagcaa acgatttcag attggcataa tgcgtagtga tcacgccgaa
    15061 tattttcttt tcattcaaca tacgcagagc tgcttctgca atggctccac ccatcacagg
    15121 atcagtgccg ctgccaaatt catctatcaa taataaggtt tcgcggtctg cgtgttgaag
    15181 gaaatgcctg atcttcatca agcgggcaga ataggtagaa agttcatctt ccagcgattg
    15241 ggtgtcgccg atatcgcaaa atatttgttt gaaaatgcca attcttgaat ggctggaagc
    15301 aggtatcagc agcccgcatt gggtcatgta ttgcaataaa cctatcgttt tcatggcaac
    15361 agatttacca cctgcatttg gacctgaaat cactaatatg cgattattgc tatcaagctc
    15421 tgcctgcaag gggacaacct gtttatgttg ttttttatgc agcgataata atacgggatg
    15481 acgtgcatcg tataaacaaa caatgggttg tttttctaat tgcggcatgc ttgattgtgt
    15541 ttccaaagca taacggccct tcgccctggt gaaatctata atgccaagta tctgttgata
    15601 tttatccagt atttgttgat gtggcgccag gtctgccgtg agggttttca aaatgcgata
    15661 gatctcgcgt ttttcttccc gttcaagttc gaaaatctca ttttgaatat tgaccacttc
    15721 ctgtggttcc ataaataccg tacgaccggt ttcgctttca tcatgtatga taccggcaat
    15781 tttgcgcttg tattcacttt gaatacctaa cactcgcctt ccattgcgca tactctcttc
    15841 cacctcgctc aaaaatccgg cactgcgata tttttgtagt gccgactgaa acacttttcc
    15901 aagcgaacgc atggcatcgt ctttttcttt cagaatgcgc acaagttctg gcgacgcgtc
    15961 attgcgcata tgccctgcat catcaataac tgcttttatc gattgagaaa tagcgcttac
    16021 ataagttgtg ccgctgacaa attgctgcaa taccggatat tgctcctgat tgctattgaa
    16081 aaatcggatg atactaccca tcaatagcgc cacgcgatgt atttcatggc attgctgttc
    16141 acttaataca tagttttcta tggcaagcag tggtagggta gggcggatat caaacatttt
    16201 atcgtgaggg aatccttggg catattgcag caaatgcgtc atttcgcgtg tttgcaacaa
    16261 catctttttc acctcatcaa atttgtccaa tggacgcact cttgcaaccc gatccgcccc
    16321 cggactgcta tcgcaatacc tggctacctc gtgtaacacc ttttcaaatt ccaatacttg
    16381 tggtatgtcc gccggaaata tcctcaccct tgatttttgc acaaaattac ggctattgat
    16441 ccattttcat acacgtagaa gaaatactat cttgcgtcga aaaccaggca tatgtgtgat
    16501 atcattgacg caaagcaatt aaattttgat cagaacaatc tgattctgct taattgtctg
    16561 cttgcatttt tgatgtttgg aatagcactt gacctaaagg ttgaacactt taaacgtctg
    16621 attcaatacc cgcgaaaggc cattgcggga attctatccc agtatgtgtt gctgccattt
    16681 cttacctatt tgattgtaat catcctacag ccttgtccga gtattgcttt aggtattttt
    16741 cttcttggag catgtcccgg tgggaatatg agcaatttcc tttcctcact ggcaaaaggg
    16801 aatgttgctc tttcagtaac gcttacagca acctcaactt tgttggcacc aattatgacg
    16861 ccgctgaatt ttgcatttta tggtggatta taccctccaa ctgctgaatt gatgcaggaa
    16921 ataagtgtcg aacctatgga attatttacc accattgttg tgatccttgg tgttccactt
    16981 attattggga tgttggtaaa tcattttatg ccaaaattca cagcattgat ccgtaggcct
    17041 gttcaaattg tttctgtcat catcttcctt tgctttattg tatttgcctt tctgaagaac
    17101 tatgatttat tcatggaaat tattggtatg attttcctgc tggtgctctt gcataacgga
    17161 ttagcaatgt taggtggata tggcctcgga gcactattta aactcgattt tgccgataaa
    17221 aagtgtttat caattgaaac aggaattcac aatgctgcac ttggtctgat catcattttc
    17281 agctttttta aaggacttgg tggaatggca gtagtagccg gttggtgggg catctgggac
    17341 ctagttacag gcttcatcat agctacttat tggtcgcgaa tggcagcgaa aaagacattg
    17401 caagcttaaa acggttattt ttgcacctgt tttactatgg acaagcggct gctcaggcgt
    17461 ttatttgaga aacagaatcc tatcccgttc agggatacca ctttgcacac ctataccaaa
    17521 ggttactgga ctgtgcagcc attcttgatt ggcattcctt ataaatatta ctattcaaaa
    17581 tatgaagtgg ccggatggga acaggttcct accgataagc ctgtaatatt tgcaatcagc
    17641 caccgcaatg catttatgga ttctttggca tttgtgaatt gcaaaggaac tcaggtatgg
    17701 caattagcaa gaggtgatgc cttcaataat cctatgatga agaagttgtt tatcttcttt
    17761 cacatgcttc cattgtggcg tgaacgggat ggcgtggata ccaaaaacat gaaccaacct
    17821 acctttgaag cctgtgccga tattctttac aacaatggca tggtggggat ctatcctgaa
    17881 gggaattgta taaatgaaga acatattcgt ccgctgaaaa aaggtatttg tcgtattgct
    17941 tttcttgccg ctgaaaaata tcaatttcaa cctgatatac atatcatccc tgttggagtg
    18001 atgtattcgg gtgccgaaaa attcaaacat acgcagctat tgaattttgg caaacctatt
    18061 ttgttaaagg aatattatga gcaataccgc agtgcacctg ctcaaacaat caatatcctg
    18121 aaagatgata tcgaaaaggc tatgaaggaa acagctacgc atgtgcctca cggaactttt
    18181 catcacgata ttgttgaatt gagtaaaatg cacgccagag aaacgctgct taacaacaat
    18241 attccggtta caccactaaa ttcttttaat gaagagaaag tgatctctgc aaagttggat
    18301 gcttatcagc atagtgatca tgaagggatg aaacgcttgg ttcaaaactt acataactac
    18361 aagcgtatac gcaatcagca taaccttaaa gaaaatacat tcgataaatc cagaagccac
    18421 actttcagca tggtgtttat ggctttgtat tttttattga tgtttccggt ttatatttat
    18481 ggtgcaatca tcaattatct gccttataaa tttccgcagc aatttgtaga gaaaaaaata
    18541 aagcagaaaa tattttggtc ttcagcgaag tatgttatca gtttattttt gtttccgatt
    18601 tattacctca tattatttat catcacctgg actgttttag gtagtattgt ttccggatta
    18661 atattcttgt tctcattccc tatttccgga aatattgcat actattatta tttcgacttc
    18721 aaaaagtggt tgtcggcgct gcgcttcaaa cgccttcctg agcatgaaaa agcagcactt
    18781 attcaggcaa gagatagcgt atatcagaat ctggcttctt tgtagttagc gtaatgcttt
    18841 aaaatcattg atgctatttg cactagatct cattaaagcc agtaaacctt cagcatgaac
    18901 ttgttccaga tgtgtttgcc ccattactac tctgatcata tacatacctt taacgatggt
    18961 gcctgacaaa tagtaattgc catcggcatt gatggatttt aataaggcgg tatttaatgc
    19021 atttagggct tcattatcat tgattccttg tgggtgatac cgaacgcaaa acatattcat
    19081 aacaggtgga gccatcaact cccattgtgt gtcggataat aattgattgc ggaaatttgt
    19141 tgcgatatca atatgctttc gcaataattc ctgtatccct gaaataccat aattgcgcaa
    19201 cacaaaccaa agttttaagg ccctgaatct tctgccaagt ggaatgcccc aatctttaaa
    19261 catggccaca ctttcttccg tgcgtttaag ataatcagga tttgcagaaa acgtattgat
    19321 caagtgattg gcatctttta caaaataaca agagcaatca aaatttgtaa ataaccattt
    19381 atgtgcgttg aatacgtaac tgtcggcatg gtctattcct tgtagaaaat gtcgatattc
    19441 attcaaaaaa aatgctgatc ctgcataagc agcatctaca tgtaaccata atttatgttc
    19501 aacacaaata ttgccaattc cctcaagcgg gtcaaaggct aaggtgctgg ttgtgcccag
    19561 tgttgcaact accatgaatg gttgtagtcc tgctgcttta tcttctgcaa tttgttttgt
    19621 taatgctgtg gcgatcattg catgtgcatc atctgtggca atttttttaa cctgcgcggc
    19681 accaattccg gcagcttttg cgcctttctc aatagaataa tgcgtttgat ctgtgcaata
    19741 catcaccagc ttgtcgtatc catgaaatcc gtgttggttc acctgaaatt gtgcatagcg
    19801 ttctcgtgcc gaaataattg cagtaagtgt gctcattgat gccgtatcat gaataacgcc
    19861 aagcatgccg tcagggagtc cggtagcttt tctcagccac tccattacgg tttgttctaa
    19921 ctcagttgcc gaaggtgagg tgtcccacat catggcatta attcctaatc ctgaaacggc
    19981 gatctctccc aatatgcccg gatagctggc attgcttgga aaaagggcca taaatccggg
    20041 atgttgccaa tgggttattc cgggaacaat catgtttaga aaatctttcc agatatcatc
    20101 catcgcttca cctgactgcg gcgcctcatc aggcaacgcc gcacgtaatg caccgggggc
    20161 aatggatggt tttacaggat attgctgtat attttcgagg taatcggcaa tccagtcaac
    20221 cacctgatgt gcctgattgc gaaaagtttc attggttaac atacactatg cagtacgatt
    20281 ggtttgtaat tattaatccc gtttccggag ggaaggccgc ccctaaacgt tggcataaaa
    20341 ttaagcctct ttttgatgca gctccattta actatgaagt aatatttact gaataccgtg
    20401 atcacgctgc gttgatagcc aagaatgccg ttcaacaagg ctttcgtcgc tttgtaatac
    20461 ttggtggaga tggtactgca aatgatgtca taaacggact ctgccaatca ggtatctcaa
    20521 cagaaatgtt caccgttgcc atgatacctg caggaacagg gaatgattgg gttcgcacga
    20581 ttggtaagcc tgatttcgca atgattgttg aggatcttat tcgacatgaa accatgaaac
    20641 atgatatagc caatgttacc tatgtaaacg ccggaaaaga ggccatgcgg tattttatca
    20701 atatggccgg actcggtttt gaaggtgcag ttgcaaaaaa gatctatgag cgctcatcaa
    20761 aatggcaggg cggcaaaatg caatatcaat tggcaatttt atcaacgctc tttgtgtaca
    20821 agcatacaca actcgaaata aaagttgatg gcaattcatc tgaaatgcag tttttgtctg
    20881 cagctatagg cagcggtaaa tttaatggtg gcggcatgaa acaattaccg gcagccattt
    20941 ttaatgatgg aatacttgac cttaccgtaa ttgcaaccat gagtaaaatg aaaatggtaa
    21001 ttagtttgcc aaaattaaaa acaggtgcac atattggtat gcaggaggtg caaacgttca
    21061 aagggaaaat cattgagatg aatagcaatc cacctgttca catggatgct gatggcgaat
    21121 atcttgggca aacaccactt cgctttcaag tggcatcgga gcagatcaac gtcattaaat
    21181 ggcgctgatc atttatgagt ctgaagataa ttgtttacct gcatctgcac attatttcta
    21241 attgtaagcc aatacaaatt atagtctgca ggatgcagat tctcaggaat aaatgctttc
    21301 ccccgaatat caggtctgct tacccaaaca atatcttcat gtatctgagc actgcaggca
    21361 ttcggtatta aagtgttggc atcgcgatcc aagccgccga tattatgatc gctgctgatg
    21421 aaatcagttg ttctgctgaa tgtaagcgga ttatgcacta caattttatc gcggtcagca
    21481 gcaattaatt ttggtttttg ttcactgcct tcaagatagc ttctccaact cacatagcag
    21541 cctgtttgac ctgcgctatc acaaggtgct atctgtgtaa aatcattttc atacacaggc
    21601 atgccaatta aatatgcggc tacaagtttg tcaaccagcg gtttgccatc aaagtaatca
    21661 cgtaacaacc attttgcatg tgtggtgccc tgactatgac cggcaataat gatgggcctt
    21721 ccattgttgt aatgttcaag ataatattcg aaggcagcct tgatatcgct gtaggctaat
    21781 tcgaaagctt ttttcgcatc cggattggta agtgaaaaat aggcattgaa tgttgcttgt
    21841 cgataccgtg gaacataaac gcggcaactg ccattgaaca cactcgcctg atgtgtaatt
    21901 gctcgcgcat cgatagtggc atttaatttc ttatcatcaa gagaagcatt ccattcactg
    21961 cgggaaaaaa actgtgtagg ataaataaaa aatacatcta cagctgcatc agcctgtcca
    22021 tcaacaacag gaaagatatt gtcatttgga atatggtcag cagaatcatg tctcgtgggc
    22081 aaagcactcc ataaatcagg gtttgcaaag tcaggcgcag cggggatttc agcttcagta
    22141 aaggttgcct ttaccggcat atatgcacac gctgataata aagaatatgc aaatgcaatt
    22201 gtaaccaatg ctttgggacc ggagatcata ggcgcaaaga tacaagcaaa aagtgcgcct
    22261 aattaaaaag aaggctgcca attcgcacca tcgtggaaca ttgttccacg gcaatggtat
    22321 agtcgctgct catgcccatg ctcagctcct taaagttgtt atcctgagca aaaaaacgcg
    22381 cttttgtgcg ttcaaatatg gcatgtaacg tggcaaattc cttgcgtatc tgctcctgat
    22441 tttcagtatt tgttgccatg cccatcaaac cacaaatttg aatgttatgc atagcatgaa
    22501 aagcttctga tgacaataaa gccattagtt caggctcatc gaggccaaat tttgtttcct
    22561 cagttgccac ataaatctgc aaaaggcatg gaattaccct attgcacgta acagcttgac
    22621 gatcgatctc cttcagcaaa tcggcactgt cgacactatg gatcatatgc acccaggggg
    22681 cgatatattt caccttattg cgctggagat gccctatcag atgccagcgg atatcggcag
    22741 gcatcagttc atgtttttcg cgcatctcct gaacccggtt ttcaccgaaa tcgcgttgcc
    22801 ccgcctcata caatggcatc aattgtgcaa ttggcctggt tttagaaacc gctatcaatt
    22861 gcacctttcg ggcatccaga aaggttttga tctcgtgata tgtgctgctc attggctaac
    22921 ttagcgcaaa aataaaccat tgcagcgatg cgattgactt ttttgatatg ttctgcgctt
    22981 ttgatgatga cctttgcctg caaacccaag gatccttttg tagtaaatga tcagatgcta
    23041 aacagtgttc ctgattctgt aattgttgtc gatagcatga tcaatatcct taccgatatc
    23101 cagctggctg aggcatatgt gaacgaaatg cgttcagact ctataccaaa ggaagatcgc
    23161 ttaaaacaat attatagtga aatattttcc atgcaccata ttgatggcac ttcatataaa
    23221 cgcagctatg attattatgt tggcaatcca attttgatga atcatattta tgtgaaggtg
    23281 actgaaaaat taaacttgat ggaaagtgaa catgctaccg taaaaaagaa tgtaaaatga
    23341 ataaagtatt caaaaatgct gatgaagcca tagccgatat tttcaatggc gctatactca
    23401 tgcttggagg atttggatta tgtggaattc cggaaaattg catctccgca ttggtgcgta
    23461 aaaaagtcaa cggtttaaca tgcatttcaa acaatgcagg tgtcgatgat tttgggatcg
    23521 gtttaatgtt gcagcagcgc caggtcaaga aaatgatcgc tagttatgta ggtgaaaatg
    23581 ctgaatttga gcgacaatta ctgagtggcg aactggaggt tgaattaatt cctcaaggta
    23641 ctctcgctac cagatgtatg gcttcaggat atggcatgcc tgcaatattt actcctgcag
    23701 gtgaaggcac agaagtagcg gatggcaagg agacacgggt gtttaacggt aaaacatatt
    23761 tattggaata tgcctttgac gcagattacg ctattgtaaa agcatggaaa ggcgatacca
    23821 tgggtaacct tgttttccgc ggaactgcaa gaaacttcaa cccgttaatg gccatggcag
    23881 gaaaaattac tattgcggag gttgaagaac tggtgcctgc aggtcaactc gacccaaata
    23941 atattcacac gccgggaatt tatgtacatc ggatatttca aggtgcagat tatgaaaaac
    24001 gcatcgaaca acgcaccatc agtaaataat tttataagga gtaaaaggaa ttatgctaga
    24061 taagaatcaa attgcaaaac gcattgcgca ggagttgaaa gatggttatt atgttaacct
    24121 cggaataggt attccaactc ttgttgctaa ttatattcct tccggaatga ctgttaccct
    24181 ccagagtgaa aacggcttgt tgggtatggg accattccca aaggatgaag atgttgatgc
    24241 cgacattata aatgccggta agcaaactat caccaccttg ccgggttctg catttttcga
    24301 tagcgcaatg agcttcggta tgatccgcgc cgggaaagtt gatcttactg ttctaggtgc
    24361 tatggaagtg gctgataatg gcgacattgc caattggaaa attccgggaa aaatggtgaa
    24421 gggaatgggc ggcgctatgg atctggtagc cagtgcaaaa aatattattg ttgctatgca
    24481 acatgtgaat aaggaagggc aaagtaaact gcttaaatca tgcaccttgc caattacagg
    24541 taggggagta gtgaagaaaa ttgtcactga attgggtgta ttcgacgtaa cacctcaagg
    24601 cttcaaacta cttgaacgcg ctccgggtgt gtcagtcgaa gaaattcaat caaaaacaga
    24661 aggtcgcctg gtagtggaag gtgatattcc tgaaatgata ctctgaaaat gcaatcctga
    24721 attatggtgt tttataatca caattacatt caggatgatg ccttacatct gttgccatca
    24781 aatcgcgcgt ttaaatatgg cgatggcatt tttgaaagtt gtctgctaaa tgaaggtgct
    24841 ttgccattaa tggcatacca taccacccgt ttaatgcaag gtatgcaatg tcttcacatg
    24901 gacgccctgc ctcatctttc tgagaaatgg ttgggtgatg tcattaaaga actggcactt
    24961 caacatggct atcaaagtgc tagggcaaaa atttctgttt ggcgtggagg cgaaggtctg
    25021 tatttaccca aaagtgattc accggagttg ctgattgagg tttttccctt gcattcaacg
    25081 ccattctcag aagctccgca acaatatgtg atagatatat atgatggttt gcaaaaagct
    25141 atccatcctg tcagcgcttg caaaacaatg aacgcacttc cctacattct tgcagcgaaa
    25201 tttgcagcat cccataaatg gaatgacgtg ctgatcttaa acactcaaaa cgaaattgcc
    25261 gatgccatca gctcaaatgt ttggttgata aaggaccata tgctcatcac aactcctgct
    25321 ttaaatggtg gtgtggaagg tacaatgcaa gcatggttgc ttgaccatgt agaggaattg
    25381 ggattttcgg cctcacgcaa atctttaact atttctgatg ttgaatctgc tgatgctgtt
    25441 ttcctcacca atgccataaa agggattatt ccggttacac ggttcaggga gaaagcattt
    25501 acgaacaatt tgcctctagc cttgttacga gatgtgaaaa ccttgctctt tgcctgaaat
    25561 aaacaatata cctgtagcaa ttcattggtt tcgtcgcgac ctgcgattga cagataatgt
    25621 tgccctttct ctcgcacttc gcaagcatga aagagttata ccattattta tcttcgataa
    25681 ggaaattctg gatcaactgg agcctgacga ccgcagggtg acttttattc atgacgtttt
    25741 attgcagatg gatgccacac ttaaaacgtg gggttcgtcc ttattagtga aatacggtgc
    25801 acctgctgat gtgtggcgct cgctgattgc agaatatcac atcgaagcag tatataccaa
    25861 tcatgatcat gatccgtatg cgttgctgcg cgatggcaat attggtgcat tgttagctga
    25921 tcatcatatt ccgtttacca cttgcaagga tcaggtgata tttgaaaaag atgatgtgtt
    25981 gaaggacaat ggcacgccat atacggtata cacaccttat tctgtaaaat ggaaatcgtt
    26041 gtttaaagag gaatatctca atgaacatca gatttttcca tttcgcaaac attttcatca
    26101 atttcaggca actcccattc cttcgcttgc catgatagga tttgatggcg agcgagctga
    26161 tattcctaaa atgttgcctg acgaagtcat cattcgcaat tatcacaata cccgcgatct
    26221 gcctgcaata aatggcactt cgcgattgag tgtgcatttg cggtttggca caatcagcat
    26281 acgttctttg atgcgtgaaa cacgacattt atctgaaaaa tatttcaatg aattgatttg
    26341 gcgagaattt tatcaaatga tactcgctca atttccgcat gttatcacaa attctttccg
    26401 cccggaatat gatcgcattc aatggcgaaa caacgagcgt gaattcgatg catggtgtca
    26461 aggtaagacc gggtttccaa ttgtagatgc aggtatgaga gagctactcg caacaggtca
    26521 catgcataat cgagtacgca tgatcgttgc cagttttctt accaaagacc ttttaattga
    26581 ctggcgatgg ggtgaagcgt ggtttgcaaa gcatctccta gatttcgacc tcgcaagtaa
    26641 taatggtgga tggcaatggg cagcaggatg tggtgttgat gccgctccgt atttccgtat
    26701 tttcaatcca tacgaacaag caaaaaagtt tgacccggaa ggtaaataca ttcgaaaatg
    26761 gattgcagaa attgacgagc ttacctatcc gcaaccgata gtagaccatg cctttgcgag
    26821 aaagcgtacg cttgatgcat ataaaattgc tttgtcgaat caataaatca ttaagccaca
    26881 atttgaagat attacccttt ttattgggtt gtatctttcc agaatatctt gctcaagcaa
    26941 ttctaattgt agaatagcag catctcctga aataattgta tctgccaggt aagcatgtgt
    27001 aatgtgttcg ctgtataggc catgcgtttc actttggttg ccgaaaattt gtaaacatga
    27061 acgtttttca ttcataaata cagaaaaatg tactttacca tcaatatttt gatattttga
    27121 gtacaaagtt gaaattgtat caatgaggtt tttgcaagac ttgccataaa agctaaaata
    27181 tgcacaattc gaatctttgc aaatcctctc tatgttttgt cgcattattg cattttcagc
    27241 atcaatatgt tgtatgctca aagtattagc aaaatcttca ggtgaagttg atgaatttgt
    27301 atgattacag gccacgcagg taatgagcag gatatacagg attactatac gcataaatgt
    27361 gcatttggat accggtccat tcataacgaa atcgaatggc attcaggtat atcagcaagt
    27421 atggctacca gatcattaaa atttttattg gtttatggaa tccatgatac cattgcatca
    27481 tactgcaaaa caatattatg aaatctctct gcactgcact tctgatctgc ctttccttgc
    27541 ctacatgggc ccaaaagtcc atcgttgtga atactgatat tgaaaaggcc accgtgtttt
    27601 tgaatggggc tcaactattt catacggctt cagttcatat cccctcagga ccttcggaaa
    27661 tcgtcctgca gggcttttca aattccctcg accaaaactc catacagggt ggggggaaag
    27721 gtgattttac tattctggac atccaatatc gattgttcta ccccgaaccg gttgatgtca
    27781 cacccgaatt atcggcaatt gacaagaaaa taaaaatcaa acaagattca attgttgaag
    27841 tagactatga tttgcgcgct attgctgcaa agcaagaggc tttgaatgtg cagaaaatga
    27901 tgttgatgaa caatgcactt ttacataata caaataatga ttcacttgaa ttacttcaac
    27961 attcaatagc atattatgaa tcaaaattga gtgaggtgtt ggacaaatta ttagagctcg
    28021 acaagcaatc atatgtattg aacaacagaa gagcagcaat tactgcaagg atatctgaac
    28081 tatatgctta tagaagtcag caagcagccg gatcaaatcc caacgctatg attcctcagg
    28141 tggttatgca agtttatgct gatgccgccg ttgatgcaaa aatggaagtg aattacatca
    28201 cgtacaatgc aggttggtat gcaacatatg atattcgtgc taacgatatc gccaaaccgg
    28261 ttgaacttgc ctacaaagca aatgtttggc agaattcagg tatcgaatgg aaaaatgtga
    28321 aattgacatg ttcaaccggg aatcctatgc agggtaatac attacctgag cttactgcat
    28381 ggtatctggg ttattaccaa tattattacg accgcgatgt gcaaacatta tcaggcgcaa
    28441 cagctccggc tgcagcaaca aaatcattgg aggaagtggc aggatatgat gattttaaag
    28501 atgaatcgtt gcaaaaatct acaaatgcat caaacttcac cacacaaaat gcaactgttg
    28561 ccaatgtcga atttgaaatc aacttgaagt atactgtgcc aagtgatgga aagggacatg
    28621 ttattgcctt gcaatcagag caattgaaaa caaaatataa ttatctgatc attccgaagc
    28681 ttgaacaatc tgcattcctg attgcacgca ttacagattg ggaaaaaatc aatttacttc
    28741 ccggcaatgc caatatttat ttcaacaata cctacgttgg caaaacatat cttaatcctc
    28801 ttactttaag tgatacactt tcagtatcca tgggtcgcga cagatccatt gaagtgaagc
    28861 gtgaattgct tgccgataaa tcaaccgata aaattttgaa tgcaaaagca cataagacaa
    28921 tgagttatac cattgatgtg cgcaatacca aagccattgg cattgagata atcctaaaag
    28981 accaagtgcc tctttcaaac gatgacgatg tgaaggtaga ggtgcttgag cagagtgctg
    29041 ctcaactcaa cgaggatacc ggtatcctca cctggagaga aaccctcgct tccaaacaga
    29101 agaaaacctg gaccttcgga ttcgaagtaa gttatccaaa agacgaacca ctcagtttgc
    29161 aataagctga ttgggtaagt gcagcagcac cccggcgctc aagtcggggt gctgctttgt
    29221 gtttgatatc cctgaatggg tgtggataac agaggtttag gctgcgctta aatcgtgtaa
    29281 ttttacatat gtttccgatg gagctcaata aaaccataaa aaatattggt ctttgtcgga
    29341 attaaaagtc tttttacaat gttatgattt agaaagcgtt aattttgcac cttccttttc
    29401 aacaacgaac gatgtacacc ctgcccaaat tcacaaaatc acttgcaatg gtggccgcag
    29461 ctatcctcct gacggtatct accagcctgt atgccggcaa cggaaattac aggaattcac
    29521 cttacggacg cgatcctaac cctgttcgtg tttactattt tggttatgac cttaccaatc
    29581 ccggttttgg aattggcacg gaagttaacc tcagctggac aaaaatggaa aaaagcggct
    29641 gtaaaggttc tcgtatatct gatcgccagt ttatgatgat tcctaatctt ggtatgttca
    29701 ccaatgaggc cggttctcaa agtatcttcg gaaaccttga gtttgcgtat gcggtaacat
    29761 atcgccacgg acttaccctt gaggtattcg gtgccggtgg ttatgcccaa atgctcagcg
    29821 aattgaccaa cgaccaggct gataactcta aaattacaaa cggaacagca tcagaaacgg
    29881 cctacagcgg ttttatgcca tctgccggag ttggcattgg ctacgatttc cagaaactca
    29941 atggcaaaga ttttccgttg gaactgaatt tccgtgggtt ggccacttct acaaatattg
    30001 gtgaaagtat tatcactcct gcttttcaga caggtttgat ctatagcttt taacgaataa
    30061 ttcagataaa attcttcttt ggggtcaagg gtttcattac ctttgacccc aaattttttt
    30121 atgaaaaaat atacacttct ggtattgacc ctcgccattt ctatgggtct gaaggctcaa
    30181 taccctctcg tggagatcta cgacatccaa tataaagatg ctgccgccct ggctgcagaa
    30241 gatgatcttt ctgcgtacga tggcgatacg gtcagaatac aaggtatcgt tacttttaac
    30301 ccttgcgatt acgccctgtc atctaccgga agccgcatgg gaacatggtt gcaggatgaa
    30361 gacggcggtc cattctcagg aattcacgta ctcatcgact tccctgccat cggttatacc
    30421 gatcttgaat ctttaaatga tgcaaccttg tttgtagata atttccaggt agggaatatc
    30481 gtagaatgca caggtattgt taactccttt gaaggaaata ctcaattctt gctgttaccg
    30541 atagaaacaa gtattgttgg cttcacaacc ttgccgactc ctgttcctgc tgaagtgagc
    30601 gagcttgaag tgagtgatgg cgcaggcgga caggtaatta atacagtcga cggagagcaa
    30661 tatgaaggac gttatgtgga gtttacgaat gtgtatgtta ctgatgttac accttcggga
    30721 ttgcgttggt tctggtactt acaggatgct gatggaaaca agattcagat ccgcgatgtg
    30781 agtggccact tccgcaatga tacttacgac gatgaatgta atatgtgggc cggcggcgca
    30841 gcaggtgaaa cgaatacacc tgatgcctat acaccgcctg caattggaac taacctggct
    30901 tatgtaagag gtgttattct ggaattcacc gctgctaccc aatatggtat cgctccatta
    30961 acattggagg atattggccc ttctttgttg tcaccaccta tcattacaga tattaccaga
    31021 acaccggtag tagcaacacc tgctgatgag gttactattt cttgttcaat tactgatctc
    31081 gacggttctg ttgcttcagc tgatctttat tacagctatg gctatggcag cactgattgg
    31141 aaccttgcaa ctatgtctaa taccggtggc gatagctggg ccggcgcaat tccgggtccc
    31201 ggtattgaca gtacttatgt aaattattat atcacagctt ttgacgatga tggcaatagt
    31261 atttcaaacc cgactgccgc tgctcccaat acatatattg tatatgccga tggtataaac
    31321 tccatcgttc aaattcagaa tacaccttat acttcaggag catctgtttg ggcaaatgat
    31381 agcattgtcg atatgcaaat cgaggccatc gttacctcaa caacgcaaac ctacgatctc
    31441 ggaatggttg ctgtacagga aggtgaaggt gcctatgaag gtatctttat caaatctgtt
    31501 cccggcgatg gcactgatgt gttgaatcgc ggcgatctga ttcgtattac ctcaggtaaa
    31561 gtaattgagg aattcggtgt taccaaactc acaaacatta cctataccaa gctttctgaa
    31621 atgaacgacc tccctgcttt ccatacaggg ttaaatccgt tggatgtaga cgccaagcta
    31681 tatgccgcta ctgaaccatg ggaagggatg cttgtgcgtt tcgacaatgc ttttgttacc
    31741 tcaaacaacg ctgatgccgc tacaggtggt gcgtttggag agtggagggt gaacctcagc
    31801 aatacccctg aagttggtat gcgttgcgat gattattcat atgagctgcc cttcgaattc
    31861 ggcacagaca gccttgcaat ggggcagtcg ctcagctata tccagggctt g
//
LOCUS       DAOQHY010000014        31025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_624_length_31025_cov_5.607879, whole genome shotgun sequence.
ACCESSION   DAOQHY010000014 DAOQHY010000000
VERSION     DAOQHY010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 31025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31025
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_624_length_31025_cov_5.607879"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLL28_02285"
     CDS             complement(<1..336)
                     /locus_tag="PLL28_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68171.1"
                     /translation="MRTLLKWATFVLYLSASVPAFSQATEAWYIRRTPPEPWTWAPVL
                     NTNITEMDNVFGIGGWNSGYFTTVAVADAFGPDSKFVFLEGGDSHALDLKAFLDSNLP
                     TIENWVYNGG"
     gene            complement(471..863)
                     /locus_tag="PLL28_02290"
     CDS             complement(471..863)
                     /locus_tag="PLL28_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68172.1"
                     /translation="MPLTDSDKQYISEMLTDALSACCPPMVLVSQSKTGMEIIGNVSV
                     PYGYSKKMIPGMYFSSFVVRKDMVSFYLFPLYGQVDKFEGLAPVAMKCLKGKTCFNFK
                     KKEQVISSEIKAMLKFGTEIWRKQGYVS"
     gene            complement(881..1579)
                     /gene="ung"
                     /locus_tag="PLL28_02295"
     CDS             complement(881..1579)
                     /gene="ung"
                     /locus_tag="PLL28_02295"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNF68173.1"
                     /translation="MSSPAGNKEIHIDPSWNNQLSNEFKQPYFAGIRDFLKKEKSAGK
                     TIFPPGPEIFNAYNLTPFEKVKIVIIGQDPYHGQGQAHGLCFSVQRGVAVPPSLGNIY
                     KEMRTDLGFDIPKHGFLESWAKQGVFMLNAILTVEKDQPASHKHIGWQFFTDATIRAL
                     NEHREHLVFMLWGAFAQQKAALLDTKRHLVLTAAHPSPFSADKGFFGCKHFSKANEWL
                     KQHGEQQIDWKIPD"
     gene            1669..3558
                     /locus_tag="PLL28_02300"
     CDS             1669..3558
                     /locus_tag="PLL28_02300"
                     /inference="COORDINATES: protein motif:HMM:NF016032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HNF68174.1"
                     /translation="MGQDTSNSKHTRNKHLHFVSALLEDITALEKMIADGLLESDIER
                     IGAEQEICIVNENFSPGDNSIDLLQKIQDPQFTTELAKYNIEINLTPRTCIEGGLIAM
                     ENELLEKFIVARNHASALNSHLVIAGILPTISRKEITLDYLTPKERYHMLSKKLRDLR
                     GRKFDLYLKGVDELHIRHDSIMFEACNTSFQVHLQISPAELVTSYNWSLAIAAPVLAI
                     SSNSPLLLGKELWSEIRIALFQQSIDTRHSIDEIREQRPRVTFGKDWIYHSITEVYKE
                     DISRFEVLINDDIEEHALQTLNENKVPQLRALRMHNGTIYRWNRICYGITDNKPHMRM
                     ECRYIPAGPTVRDEMANAAFWIGLMKAKPENVKKIWEHFDFKDVKSNFFKAARAGMES
                     AFVWRGKTISAYDLIKNELLPLAHAGLQNCGMPEEEIYLYLGTIEKRLETRTGAQWQV
                     QNYRRLKKEMNDADALRALTESMYTLQLQNIPVCNWPDIAVAEAVASNIAKMHTVRQL
                     MSTDVLTVYPEDPVALVARIMEWNDINHIIVEDRKGHIHGVITSGHIMELKEKQTNLD
                     ILPVKQVMRTELHTTSPDAPIESALEIMQTHWISSLPVVSENKLMGIVTKNDMLRWMS
                     FRDKR"
     gene            3626..5797
                     /locus_tag="PLL28_02305"
     CDS             3626..5797
                     /locus_tag="PLL28_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNF68175.1"
                     /translation="MRNMLVIAGMCTIVSACTNNKPQTEDDVKGPGKVTYPVTATADS
                     SSDFFGTTVADPYRWLEAEANDDPKVRQWVTDQNKVTFDYLAEIPYREQIKQRMLDLA
                     NYPKMTTPSRAGDYYYYQKNDGLQNQNVTYYRKGINGEEKVFLDPNKASADGTLTQSM
                     AGFNQKRTYVAINEAKAGSDWNTMYVKDVVNNTMMADKIEWTKFSGAAWQGDGFYYSA
                     YDKPSKTYTGSSENQKVYYHKIGDPQSKDKLIYTDPKHPLYYFSAQTTDDERYLFIYV
                     SPGTSGTEILWKDLRTNAKSFTKLFEGFDYEYTILDNDGDDLLVMTNYQAPNYRVVRV
                     DPTKPAPENWTEVIAEQKELLSGVSLAGGYLFASYLRDVKPVIIQFDRYGKPVRQIGL
                     PNNGVGGTVAGFNGEKDDKTVYFSFTTFLTPADIYSYDIESGKIELYKKAEVKFNSSD
                     YTIKQVFYTSQDGTKVPMFIVHKKGIKLDGSHPTLLYGYGGFNIPIQPSFSISRVALL
                     EQGFIYAVANIRGGSEYGEEWHKAAMFEKKQNVFDDFIAAAEYLIDQKYTSNTKLAIQ
                     GRSNGGLLVGACMTQRPDLYAVALPGVGVMDMLRYHRFTVGWGWAVEYGCADSSKEAF
                     DYLSKYSPLHNIKPGTKYPATMVTTADHDDRVVPAHSFKFAAALQAAQTGDAPTLIRI
                     DTQAGHGAGKPLAKQIEEDADWISFTMWNTGIKHMTITDKP"
     gene            5890..8205
                     /locus_tag="PLL28_02310"
     CDS             5890..8205
                     /locus_tag="PLL28_02310"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNF68176.1"
                     /translation="MKILFPLLTLTVSTLSILAQSNDYQRVVNAGTLPSEVYTPSTEK
                     FKTTIEDLDSDLSKEEAKVQKQYYLESGFGIDEMMRSGTVLYNPEYNDYLNRIADELL
                     KNDPALRGDVRFYILRSPVVNAFAGAGGNIFISMGLIAMLDNEAELAFIMGHEIGHIA
                     KDHGLDYFVTASDIDKKSTDNALFKKSSSFDPTSIAKNMYSQALETEADDYGIDILRK
                     SKYWADTNALNAAFDVLKYAYLPYENEAFPISFFEGEHYKISNNLKLDSIKPITGTPE
                     LLTKKEALKSTHPSIGERRKTVNQKLGNLTNENRQAYIVSEADFRKLRNAARYELPMF
                     YLHNEFFQDAIYLSYLGLQKDPGNKYLEKIIGKSLTAYSKFRNSKEDEVYAEKARFED
                     YEGEQQQLYYLLWAINDAELNTMALLYNFKLHLKYPEDPEIIPQCQALVNDMVFYHFN
                     DEEDFLMGKNISTDSLYLLSDQANPKLKVAPPSSTSKVKTRKASVRSKRTTAAKVKID
                     KNKKQLLYAFNDYWENKDFRRLWNEAVVERDKREAEAKDLRKQGYKIKDTGDKRDYFF
                     GQKLGVEKVIVVNPFYKRIDLRKENAVEYVSSEEGELNYLEVLKTNAALVDIQLEILD
                     PLTLQATDVEKFNDMNELNDWFSEQLDFGSINLPGYNQAVIDSIATKYGTDYFLWTGI
                     VSLRDKQNLLGPILYIALSPAFIPLLPYGIYELVTPEYEFFYLSLLYDVKTHEGNVLK
                     FLFLKNNDSRAILNSHTYEMLNQIAAKPNNN"
     gene            8220..8984
                     /locus_tag="PLL28_02315"
     CDS             8220..8984
                     /locus_tag="PLL28_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68177.1"
                     /translation="MKKLCIAILLTAGCYQLGLSQAVGYQGKKFIVEAGFSPATNLLL
                     RYLDYSMVNDAIPEGERAPTEFKIIPRVSFEAVIFNSGSFFVRANPYNYSSTLSYLDK
                     NYDEVHLMQLDTKGFMFSIGYKSYNTPTPAPLGSYWGFNVTRYNFNSSYENSPDDSQG
                     LPNEISDYLIAESGCWGLFVSYGAKNIFWDKMTLDISLEGGYFFNNPEVGLTMDDNAF
                     GNDFEPIHLEYPQSINLTNTRAFFFIMPTIQLGYLF"
     gene            9046..9118
                     /locus_tag="PLL28_02320"
     tRNA            9046..9118
                     /locus_tag="PLL28_02320"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9079..9081,aa:Arg,seq:tct)
     gene            complement(9235..10179)
                     /locus_tag="PLL28_02325"
     CDS             complement(9235..10179)
                     /locus_tag="PLL28_02325"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNF68178.1"
                     /translation="MPITSTDFKLPGATAVYHGKVRDVYTISDNLLVMVASDRISAFD
                     VILPRPIPFKGQVLNQIAAYFLGATSDIAPNWLISTPDARVAIGHKCEPVKVEMVIRG
                     YLSGHAAREYKSGKRTICGVDMPEGMKENDKFPEPIITPTTKAAAGHDEDISREEILR
                     QGLVSEADYVQMEKYTRALFARGTEMAAKQGLILVDTKYEFGILNGEVVLMDEIHTPD
                     SSRYFYLEGYAGRQERGEHQKQLSKEFVREWLIENGFMGKEGQQVPEMSDAWIKTISD
                     RYIELYEKVTGRAFEPIDNSLDEINNNIVTELAKLNGR"
     gene            complement(10210..12798)
                     /locus_tag="PLL28_02330"
     CDS             complement(10210..12798)
                     /locus_tag="PLL28_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68179.1"
                     /translation="MRKRIIIISSIATVIILLTLGWYFFIHKRYSGVMNAMPDDVICF
                     FDINKSESFNEFIQHDPAMQSLADLQLFGKLYNDYALYRAILASDPDLLADITNGHGL
                     AGAFSTGEGRVDYLFLSDLNNAKRFKSKALLPELNGQKPTVRTHTFEKETVYELHYAD
                     LNITLSFVVTNGMLIYSTASVLVENSLHQLKSGDPVSEQSSFSKVKQELPDNQPYTLY
                     IHLPRFGEFLASFTGNQQYASVMQVAKFADWIGCTLTNTEKGISLNGFCAADNATISR
                     YSGAFSSDMHPAVPANTAVLFRIQTEQLAERLSTGMTGEEVNRSFFDNWSGWMGSQLI
                     IGISESLDKNYMRRAFAIIPAQDKQLAVSKINKISIQDTALYRNHPIFAMAQGDVLQA
                     ILHLPVPDTCFATWEEGDLVLACDRQQLLQMIDAVENKGTLTEQAEYQSFKGNVSASF
                     NTSIFLHLASSEQLIRQMVSDKQIDSIAKNFGLLQLFPRIELQFTEHKDMYMFNGFIS
                     YTATAERGAGMLWTTQIDAPVEAGPFAVMNDITGIRNILVQDTAHQLYMISGNGDVVW
                     KRPLPSAILGEVHEVDFYGNDKTQFLFNTGESIFLIDVRGDAVEGFPITLTTRISNPV
                     ALAMSGKSDYRMFVACENKNVYGYYKDGKPIAGWNPLKSSGGINLPMFILPTSKGGVP
                     GYVDARSLAFFKANGSSLYTQKIPSPLMKELMVMDSTVFMLDTMRTVFWVDSDFNGNN
                     SKLASESTQCRLLPGFADSSMGVVMLQNGFIKAAGLSGEPTVVSEPKGMVDQLVVLKG
                     PDRWFYGYTTTTGELALIDKQGQLLSGFPMPGSALSAISDLTLNGDAILITAVGSQLH
                     AYRIHE"
     gene            12875..13282
                     /locus_tag="PLL28_02335"
     CDS             12875..13282
                     /locus_tag="PLL28_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HNF68180.1"
                     /translation="MNDKSIIRIAAWSGALSVALGAFGAHGLESMAESGKIDNADLHT
                     FETAVRYQFYHTFALMAVGLLHNYLSEKSMRRIAWLFIAGILIFSGSLYLLTFSPVLF
                     GERMGWFGAITPLGGLSFIAGWIMLALQFRKSR"
     gene            complement(13361..16015)
                     /locus_tag="PLL28_02340"
     CDS             complement(13361..16015)
                     /locus_tag="PLL28_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF68181.1"
                     /translation="MKKIFAILTIVLFASKAFAQPVDPDVDDMNVTRKLMYGDALMSA
                     GSYYNAQDVYKKAYDDEASEESAYKLARAYYLARNYKEAEKWYKTAMDAGKGKGGAYP
                     EAGFYYAMSLKYNGKYAEAKEAFKAVSKNSNLKGTTGTALKRQAKNEDKGCDLALELG
                     KKPADVNVEHLGSNVNNNYTDLSPRYNPDGDLTYASLVSEDVIDMGSQKKTELKARIF
                     ESKMEGESWAKSKEMSAPFNKGAAHTGNGAYSLDNKRFYYTECNPNDSLVMVCKIFVS
                     EFEAGEWSNGKEVSQVNESGFTSTHPTVGTFKGKEYLYFSSNRTGGRGGMDIWYSEIQ
                     NKGKSYSNPMNCGTKVNTFGDEITPFYNATTETFYFSSNGQINIGGFDIYKTKGGAKT
                     FEAPMNMGLPFNSSVDDFYFATDATGKKGFFVSNRKGGFSVKSETCCDDIYAYKYPPE
                     FVIMARAIDMNTNEPIYGASVDLNQSGVKRDSSVTKTANTYEDFYVGTAQDKFEIVAH
                     KEKYKNGIATTSTVGLKESDTLFVDIMMEPIIEMKPIEVKNIYYTTGTWNLRPESFKA
                     LDSLYNILQLSPQLQVEIGSHTDSRDTEEKNRILSEHRADTVRSYLISRGINPDRLIA
                     KGYGETTPKTITSEFKVPGGTTIPNGTVLTEAFINKFKGDDYEYLHQLNRRTEFTIVG
                     VIPNAIITYDEATIEANEARKREEEEKRNKRLQDLQQVDETDTPDTGGTETPKTGTTG
                     TKGNKGTTTTTTAASTLDPTMTKKGNMFEGNAAVNGKNQTKYILNMSPSITIAMVGKD
                     YFIQLWESGAITAADIKNDKPTDLGNGTTVKGDVFTIKTLQLGDITLTNVDTKINLTS
                     TQNLIVGVKVAEKGGCEFDTTKMKFKCK"
     gene            complement(16065..17081)
                     /locus_tag="PLL28_02345"
     CDS             complement(16065..17081)
                     /locus_tag="PLL28_02345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNF68182.1"
                     /translation="MMKLYKIACLAACMAVGVLQAQDIHFSQYNASPLALNPALTGVN
                     SCDWRIGATYRNQWNSVTTPYVTYEVFGDMPLIKDIGGASKLAGGLLLYNDVSGDGDL
                     TNLSAMGNVSFVLGLGGQTNHNLSIGVQAGLMHKSLDFNALKWPNQWDGDEFNTAIDP
                     LENIGENISRFNMGAGIAYHNSVSKTFNITGGVGLFNLISPKETFLQDEENKLGMRIS
                     GHLKAGITIDGKYGIIPSVLYQSQSGAKEIIVGSDFGYFLNSANFPATFFLGGAYRLD
                     DAVIASVGMDYKNFRFGASYDINTSTLQDASGGKGGFEISLVYTGCILPVIPKNYVMP
                     CPRY"
     gene            complement(17258..19102)
                     /locus_tag="PLL28_02350"
     CDS             complement(17258..19102)
                     /locus_tag="PLL28_02350"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HNF68183.1"
                     /translation="MKTVLRNLLCALSVLPFLTLPLNTYAQLNVDDTYTAEELVSTLL
                     GEGVTVSDITIDCPGGAYGYFDCVDCNVGIPNGILLTSGSVGNAVGPNNSPSQSSSLG
                     MPGDPDLDMIPGVLGTNDACVLEFDFTATSDTIRFNYSFGSEEYLEYVGSFNDVFAFY
                     ISGPGIAGTDNMALIPGTATTVSINNVNDAVNSEYYNINGTGFNAPFNSDDYYIQYDG
                     FTTVLEAKHAVFPCETYHLKMAIADDLDDALDSGVFIEAGSLTSPGVTVEYNTDIEGY
                     PYIIEGCNEGMITFSLSFAPVDTFTVTLNVDGTALMGSDYTTFNNVLEFLPGDTLIEI
                     PIVAEEDGIDEGVETIIITLDLGCVAGVGDSLVVLVYDALPLLVSADTLICPGTEATL
                     TASGADSYSWSPAETVTDPTEATTTVFPDENTTYTVTGTLASCVNTAETTVEIQEPTA
                     DAGADTTIYLGEFASLDADGGVEYSWSPSTGLSETDIENPTASPDFTTTYTVTVTTAI
                     GCEFTDQVTVTVSSDAMVGVPTAFSPNSDGVNDGFTIIVRGQVATWLLQVYNRWGQLV
                     FETDNYAAAWNGTFEGSEQPLGSYVYVLNYKDMNGESFVQQGNFTLVR"
     gene            complement(19255..20556)
                     /locus_tag="PLL28_02355"
     CDS             complement(19255..20556)
                     /locus_tag="PLL28_02355"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNF68184.1"
                     /translation="MILLRSYANLGEFFNAVFGTNMRLPVFSFGFFVGLAFMAAGYIL
                     YRELRRKEKAGIFHAEKVKVMVGEPASAFELISNAVFGFILGFKIGGMIFNWQTFNDD
                     PQSFMFSGEGNILVGLLGAALLAYSKYREKKKEQLPQPQEKTLLLYPSARLGDFVMLA
                     AIFGILGAKMFSNFEEENGWSEFLQNPFKNFFSGLTIYGGLILGAAAIIYYAIRKKIN
                     VLHLGDAVAPALILAYGIGRIGCQVSGDGDWGIINSAYISQENGTLREAEKGEIDSVI
                     QIHAAYYIREFDSLSNVKSVYVKAPGFLPRSLVAQNYAYNVNKEGMYIAKCQGDYCGQ
                     LPASVFPTPIYETIMSLMIFGMLWWLRRRVTVPGMILAIYVIFVGIERFFIEKIRVNN
                     VLEFAGIRATQATFISIIFVIFGLVMALVLVQVEKRRRLSH"
     gene            complement(20553..21911)
                     /gene="mtaB"
                     /locus_tag="PLL28_02360"
     CDS             complement(20553..21911)
                     /gene="mtaB"
                     /locus_tag="PLL28_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNF68185.1"
                     /translation="MPSVALYTLGCKLNFSESSTLGRKMQDAGYMVKGFHEAADIYVI
                     NTCSVTDHADKKCKKVVKQALRQSPQARIVIVGCYAQLKPQEIAEIPGVDLVLGAAEK
                     FNLVEHIAALPEKQSSGSGAVMAGKVEDVAGFHAAWSFGDRTRTFLKVQDGCDYSCSF
                     CTIPLARGASRSNIISRVVEQANTIANQGVSEVVLTGVNLGDFGLRNATENNESFYEL
                     ICSLEDTNIQRFRISSIEPNLLHEQIIKQVSNSKKFVPHFHIPLQSGSNKILRLMRRR
                     YVRELYAERVGMIKSLMPDCCIGVDVITGFPGETHEDFIDTFQFLQTLDISYLHVFTY
                     SERPNTVAIELPDSVPQQIREERTAQLRNLSEKKRRAFYEQHQHTTRPVLFEEETDGQ
                     WMFGFTDNYIRVKTQYDPLLVNEIVHVALGEIDAEGHMQAIAIDQTENSHQFHHLQAP
                     EL"
     gene            22122..23651
                     /locus_tag="PLL28_02365"
     CDS             22122..23651
                     /locus_tag="PLL28_02365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68186.1"
                     /translation="MADIDSDGDMDLVGYIARDGNPTVLAWLENEGTPPFVQHDIAAT
                     EYGYYIHTTDFDMDGDMDILAPIQLDGTVLYFYENHGDGDFVPHELTYLSGYYRNFAV
                     SDLDGDLDPDILRIAPSIGGVMFDVTWLENDGDGNVTSHIIDNINTPESFGAGDLDDD
                     GDMDFVLGTANRVYWYEKIVAGYTRHLVINSGDIFFNKIFIEDMDLDGDKDIISVSDY
                     IIKQYENDGSENFTTSVAYDPPGGAILIGLGDINGDSFPDMFGKWVITGNYHVINLLG
                     NADFTFTLMDSIVPICGNEALTYGDLDRDGDTDYILSENCEVGPIKDVMFWELNGSTP
                     LPCAPVEVLNVYTDSLGLQATWSDTYADYYKLFAKCIDCPAPSPTISKTVYAPIGYLD
                     DMLPCHTYKVKVRAFCGGSVYGSPVLTVTTDGAACRQSEVDIESVLSVYPSPASHTLM
                     ISGIQIDVRAEIYAMDLSGKTLRLENSANEIDIRMLPSGFYILTIELNGDLYRTQFIK
                     Q"
     gene            23654..24388
                     /locus_tag="PLL28_02370"
     CDS             23654..24388
                     /locus_tag="PLL28_02370"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNF68187.1"
                     /translation="MILQHGARQFDLSNSRSIAIPYSTGEQVNCYYAPPFQVTPVVMG
                     TFIGDINQGSPVNYMNVQVNPHGNGTHTECIAHISNMPVNIQGVLKNHHFIAQIISVT
                     PDIIENGDTVLLSKNIIPILEKDIEAVIIRTLPNESAKTTKAYSGTNPPYLEATAALS
                     FRESGIKHILIDLPSLDREEDGGALAAHKAFWHYPEAPRLDCTITELIFVPDAIADGI
                     YLLNLQIAPFCMDATPSNPVVYPMLK"
     gene            24470..26707
                     /locus_tag="PLL28_02375"
     CDS             24470..26707
                     /locus_tag="PLL28_02375"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNF68188.1"
                     /translation="MRKLLSTLTLIALTISVSLAQMTIADARLQPVGTSVTVKGIALN
                     GPELGSIRYIQDATGNIAVYSATLVTGVQRGDSITATGPLFNYNSLLEISPASSVTIN
                     STGHPLPAPIDVTSLSGWVEGNEGKLLRISNATFVDAGTFSTAGSGTNYDVTDAAGTS
                     QIRVVTTTNIDGTPIPAEPVFITGIMSQYAPGGTGGYQLLPRDLADISTGGNPPVISS
                     VLTQTDITTSSFTVNFNTLYEGNTIIYYGTTMSLGSTASSAAMVTTHALNLTGLTAGT
                     VYYVKAASVSASNDTSYSAITAMATASNSSGQIKVWFNNPVDNSVSTGTNATFVNSAM
                     DDTVIWYLDHAKYSLDIAIYNIDNVNYIINSINDAYARGVDVRVICDNGVDDFNYGLI
                     NVGVGKKKKSPPDGSTNADGALYGIMHNKFIVVDGISVDPDDAWVLTGSMNFTDQQIK
                     MDKQNYLAIQDMSLAKAYRLEFDEMFGGKFGPDKSNNTPHEFMIGGKRVEVHFSPSDE
                     VETRMIERIGTADYDMHFAIYSFTRFGISYAIEDAVLDRGVFASGIWDETNADDSTAI
                     NVCEDAMGDRFEKYSGSNLLHHKYLIIDPNCPQSDPMVWTGSHNWSTSANSRNDENTI
                     VIHDATVANLFYQEYMQRYKDEGATDMVNAACEVAINNSTQDEGEIRLYPNPATNQVT
                     IALGEVTNGNIKIFDISGKVMLSSEVHGLQYFTININELPAGMYFVVCDVNGGNKTSK
                     LIIRD"
     gene            complement(26759..27406)
                     /locus_tag="PLL28_02380"
                     /pseudo
     CDS             complement(26759..27406)
                     /locus_tag="PLL28_02380"
                     /inference="COORDINATES: protein motif:HMM:NF016850.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CmcI family methyltransferase"
     gene            complement(27463..28044)
                     /locus_tag="PLL28_02385"
     CDS             complement(27463..28044)
                     /locus_tag="PLL28_02385"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF68189.1"
                     /translation="MEREVKGCSNLLDIGCGSFSPVQTFNKKLYCVGIDAFAPSIEKS
                     KTLGIHNEYHQIDVLDALEKFGPKSFDVVVALDLIEHLRKEDGLKLLEMMDKLARKKI
                     FVYTPNGFLPQGDRENNPWQVHYSGWSPEEFGAFGYEVLGVNGIKSMRGEFAKPKLKP
                     WFFWAFISDLTEPFVKYKPSKAFQLLAIKRLDK"
     gene            complement(28127..29119)
                     /locus_tag="PLL28_02390"
     CDS             complement(28127..29119)
                     /locus_tag="PLL28_02390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF68190.1"
                     /translation="MSEYYCLIVTYNNRWQFLSRVLERLGENVGLNVVVVNNGSTDFP
                     ETQASVLNFNTYKRIDFTENVGSAKAYGEGLKYIRNVCPDAIVWMIDDDNLPENGAFA
                     ILKERLFAEESLHGHQNIMVLALRPSRNYLTRIVAGENIEKVLSYGNDFLGFSFKKLA
                     YKSLPKLNMKIDSSRTNSITLPYAPYGGLMFRSGVIDKIGYPDERFFVYADDFDFTSR
                     LAKIGGEIIIEPRAVVHDLELSWQSTSANTSKLPLALRGSPFRVYYSTRNFIFFQKQR
                     LVKSQLAFAFNALIYILYLSLRAALSGKFAGFAVFLNAVIDGWRGNFDNSKYRP"
     gene            complement(29265..30755)
                     /locus_tag="PLL28_02395"
     CDS             complement(29265..30755)
                     /locus_tag="PLL28_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68191.1"
                     /translation="MKTLRLFLRSLILIFVLSLLPNKCIGQPNEIGSGIAINFPGTVG
                     SRVDLGDVYNSVNFPLTIEAWIYPTAWMPSVYSPVMASDNYGPGGHYYGFWLRFNTSG
                     NLIFEIGDGYGAGSSDRRGKKTIGVPPLNAWTHIAVVAISVNDINFYFNGVLQSTVDT
                     DGGAINTTILHSSYPAYIGCQEHPAAEVNFTGNLDEVRLWDIARSESEIRDKMCEKLT
                     GLEPGLIGNWRFDESYVSSTVADYSSTNTDGYVIGSAVKMTSGAPIGDLSVLVYSDDY
                     LGVTLDLNSIGGDHLKLSNITNVPFGMHLYRVDQYPYSMTGLQEIPEYYYGVFIANDG
                     ITAKYSLDYSYSFSNGVVVPENESSSKLYKRLNNSYDIWNNSLSTLFIAANKIKKKKH
                     AGDCEFVFTVDSITNINIVNSIVGNNAESYSLMVYPNPSTESIICQGLEVDTEFYIIN
                     TLGEEIFRGTPLQTGCQIDVSTYPDGTYFLVQNKGGVVYKTIIMKQ"
BASE COUNT         9296 a         6433 c         6139 g         9157 t
ORIGIN      
        1 accgccgttg taaacccagt tttctatagt aggcaggtta ctatcgagga aggctttcaa
       61 gtccagtgca tgactatcgc cgccttcgag gaacacaaat ttagaatccg gaccaaaggc
      121 atcagcaacg gcaactgtgg taaagtaacc gctattccag ccgccgatac caaaaacgtt
      181 atccatttct gtgatattgg tattgaggac cggagcccat gtccacggtt caggaggggt
      241 tctgcgaatg taccaggctt ctgtagcttg cgaaaatgcc ggtaccgaag cagacaggta
      301 taagacaaat gttgcccatt ttaggagtgt gcgcatgtgt taggtgggtc taagcggtta
      361 taatcgctta aaccgtaatc ctaaactaga aatagtcttt gaactctcaa agcaaatagg
      421 caaaaaaatg gtgcaaaacc atcattttca gtgcaataag cgcttaaacc ttatgaaaca
      481 tagccttgct tccgccaaat ttccgtgccg aatttgagca tggccttgat ctctgaagaa
      541 attacctgct cctttttttt gaagttgaag catgttttgc cttttaagca cttcatggcc
      601 acgggtgcca gaccttcaaa tttatctacc tgaccgtata gaggaaagag ataaaaacta
      661 accatgtctt ttcgaaccac aaaagatgaa aaatacatgc ccggaatcat ttttttggag
      721 tagccataag ggactgaaac attgccgata atttccattc cggttttact ttgtgaaacc
      781 aaaaccatgg gagggcagca ggcgcttaag gcatctgtaa gcatctcaga tatatattgc
      841 ttatcgctat cagttagtgg catacatgta ttgttaagtg ttaatcagga attttccagt
      901 caatttgctg ttcgccgtgt tgtttcagcc attcatttgc ttttgaaaaa tgcttacaac
      961 cgaagaaacc tttgtcggca ctgaacggcg aagggtgtgc cgcagtcaga accaggtgtc
     1021 ttttggtatc aagcaatgca gctttttgtt gagcaaaagc gccccaaagc ataaaaacca
     1081 aatgttctct atgctcgttc aaagccctga ttgttgcatc ggtaaaaaat tgccaaccaa
     1141 tatgtttatg cgatgcgggc tgatcttttt ctaccgtcaa tattgcattc aacataaaaa
     1201 cgccttgttt tgcccatgat tcgaggaagc cgtgtttggg aatatcgaat ccaaggtcag
     1261 ttcgcatttc tttatagata ttgcctaatg atggcggcac ggcaacacct ctttgaacac
     1321 tgaaacacag cccgtgtgcc tgtccctggc cgtgatatgg atcttgtcca ataattacaa
     1381 ttttgacctt ttcaaaaggt gtcaggttat aggcattaaa aatctcagga cccggaggaa
     1441 aaatggtctt acccgccgat ttttctttct tcaaaaagtc gcgaatccca gcaaaatatg
     1501 gctgcttgaa ctcattgctc aattgattgt tccacgatgg atcgatatgt atttctttat
     1561 tgccggcagg tgaagacata gtggtcaggt agacaacaat tttaagcaga aatgatgaat
     1621 atcaccaaca accccatagg tttgtgataa atttgattgg attactccat gggacaggat
     1681 acaagtaata gtaagcatac gcggaataag cacctgcatt ttgtcagtgc tttgcttgag
     1741 gatatcactg cgttagagaa aatgatagca gacggtcttt tggaaagcga catagagcgc
     1801 attggagccg aacaagagat ttgtattgtt aatgaaaatt tttctcccgg cgataattct
     1861 atcgatttac ttcaaaaaat acaggatcca caattcacca ctgaactggc aaaatataat
     1921 atcgaaatca atttaactcc ccggacatgc attgaaggcg gattaattgc catggaaaat
     1981 gagttgttgg aaaaattcat tgtcgccaga aatcacgcct ccgcattaaa cagtcattta
     2041 gtaattgccg gaatattgcc aactataagt cgcaaggaaa taacacttga ttatctcaca
     2101 ccaaaggagc gttatcatat gctcagtaaa aaattgcggg atctgagagg tcgcaaattc
     2161 gatttgtatt taaaaggtgt tgatgaatta catattcgcc acgacagtat tatgtttgag
     2221 gcatgtaata ccagttttca ggtgcacttg cagatttctc cggccgaatt agtgacatct
     2281 tacaactggt ctttagccat tgcagcacct gtgttagcaa tcagttctaa ttcaccactt
     2341 ttgttaggaa aggaattgtg gagtgaaatt cgaatagcat tatttcaaca gagcattgat
     2401 acacgtcaca gtatagatga aatccgtgag cagcgacctc gcgttacctt tggtaaagac
     2461 tggatatatc attcaataac ggaagtatat aaagaagaca tcagtcgttt tgaagtattg
     2521 ataaacgatg atattgagga gcatgcgctt caaacgttaa atgaaaataa ggtgcctcaa
     2581 ttacgtgctt tgcgtatgca caacggaact atctaccggt ggaatagaat atgttatggt
     2641 attaccgata ataaaccgca tatgcgcatg gaatgtcgct acattcctgc aggtccgacc
     2701 gttcgcgatg aaatggcgaa tgcagcattt tggattgggt tgatgaaagc caaacctgaa
     2761 aatgtaaaaa aaatatggga gcattttgat tttaaagatg taaaatccaa cttctttaaa
     2821 gcagcacgtg ctggcatgga atcagcattt gtatggcgag ggaaaactat ttctgcatat
     2881 gacctgatta aaaatgaatt attgccattg gcacatgcag gattgcaaaa ctgcggtatg
     2941 ccggaggaag aaatttattt atatctaggc actatagaaa aaagattaga aacaagaacc
     3001 ggagctcagt ggcaagtgca aaattaccgc cgtctgaaaa aggaaatgaa tgatgcagat
     3061 gctttgcgcg cgctgactga aagcatgtat accttacaat tacaaaacat tcctgtttgc
     3121 aactggcctg atatcgctgt agcggaagca gtggcatcta atattgcaaa aatgcataca
     3181 gtaagacagt tgatgagcac tgatgtgttg actgtatatc ctgaagatcc tgttgccctg
     3241 gttgcaagaa ttatggagtg gaatgacatc aatcacatca tagtagaaga tcgcaaaggc
     3301 catattcatg gtgttattac ttcagggcat atcatggaac tgaaagaaaa acaaacgaat
     3361 ctcgacatat tacctgttaa acaagttatg cgaacagagc tgcatactac cagcccggac
     3421 gccccaatag aatcagcgtt ggaaatcatg caaacccatt ggataagcag tttgccggtg
     3481 gtgtcggaaa acaaattgat gggaatagta acaaaaaacg atatgctcag gtggatgtcg
     3541 tttcgcgata agcgttaaat actgttagca gggttcatac tgccggcaat ttgagtaatt
     3601 ttactcctat attattgaaa accatatgcg aaatatgctt gtaatcgcag gaatgtgcac
     3661 tatagtgtct gcctgcacaa acaacaaacc tcagacggaa gatgacgtaa aaggaccggg
     3721 taaagtaact tatccggtga ctgcaaccgc ggattcctcc tcagattttt ttggaacaac
     3781 ggtagcagac ccttaccgtt ggctagaagc tgaagccaat gatgatccga aagtgcgtca
     3841 atgggtgaca gatcaaaata aggtaacctt cgactatctt gctgaaatac cttatcgcga
     3901 gcagatcaaa cagcgcatgc tagacctcgc caattatccc aaaatgacaa caccaagtcg
     3961 tgccggtgat tattattact atcaaaagaa cgatggctta caaaaccaaa acgtaactta
     4021 ctatcgcaag ggaataaatg gcgaagaaaa agtttttctt gacccaaaca aggcatctgc
     4081 cgatggcact ttaacccaat cgatggccgg attcaaccag aagcgtactt acgtagcaat
     4141 aaatgaggcc aaggcgggaa gtgattggaa tacgatgtat gtaaaagatg tggttaataa
     4201 taccatgatg gccgataaaa ttgaatggac aaaattcagc ggtgcagctt ggcaaggtga
     4261 tggattttat tacagtgctt acgacaagcc atcaaaaact tataccggca gcagtgaaaa
     4321 tcaaaaagta tactaccata aaatcggcga tccgcaatca aaggataaat tgatttatac
     4381 agatcctaaa catccattat attatttcag tgcacagact acagacgacg aacgttatct
     4441 gtttatttat gtgagtccgg gcacatcggg aactgaaata ttatggaagg atttgcgcac
     4501 aaatgcaaaa tcatttacca agttgtttga aggctttgat tatgagtaca ccattcttga
     4561 taatgatggc gatgatttat tggtgatgac aaattaccag gcacccaatt acagagttgt
     4621 tcgtgtagat ccaacaaaac ctgctcctga aaattggact gaggtaattg ctgaacaaaa
     4681 agaattactt tcaggcgttt ctctggcagg aggttatttg tttgcttctt atttacgaga
     4741 tgtgaaaccg gtgatcattc aatttgaccg ctatggcaag cctgttcgtc agattggctt
     4801 acctaataat ggtgtaggtg gaactgtggc aggatttaac ggagagaaag atgacaaaac
     4861 agtttatttt tctttcacca cattccttac acctgctgat atttattcct acgatattga
     4921 atcaggtaaa attgaattat ataagaaagc tgaagtgaaa ttcaacagca gcgactatac
     4981 cattaaacaa gtgttttata ctagtcagga tggcactaaa gtaccgatgt ttattgtaca
     5041 caaaaaaggt attaaactcg atggcagtca ccctacatta ctttatggat atggtggatt
     5101 taatattcct atccaaccat ctttcagtat cagcagagtg gcattgttgg agcaaggatt
     5161 tatttatgcc gtagccaata tcagaggagg aagtgaatat ggcgaagagt ggcataaagc
     5221 tgcaatgttt gaaaagaagc aaaatgtatt tgacgatttc attgctgctg ccgaatactt
     5281 gattgatcag aaatatacat ccaacacaaa gcttgccatt caaggtagat cgaacggtgg
     5341 tttgcttgtt ggtgcatgta tgacccagcg tcctgatctc tatgctgttg ctttaccagg
     5401 agtaggtgta atggatatgc tccgttacca tcgctttact gtgggctggg gatgggctgt
     5461 tgagtacgga tgtgccgata gttccaagga ggcatttgat tatttatcaa aatactcacc
     5521 tctacataat atcaaaccgg gtacaaaata tccggctact atggtaacca cagcagacca
     5581 tgatgacagg gttgttcctg cgcacagctt taaatttgct gcggcactac aggctgccca
     5641 gaccggggat gctccaacct taattcgaat cgatacacaa gccggtcacg gagccggtaa
     5701 accactggcg aaacaaattg aagaagatgc cgattggata agcttcacca tgtggaatac
     5761 gggaattaag catatgacca taactgataa accataacat gaaataattt gcatataagg
     5821 aggccttgag cctccttttt ttttacccat atttttcata aacttgcata cagcctaact
     5881 aactcataca tgaaaattct tttccccttg ctgactttga cagtgtcaac tctgtcaatt
     5941 ttggcacaaa gcaatgacta tcagcgcgta gtaaatgccg gcacccttcc ttctgaagtg
     6001 tatacacctt caactgaaaa attcaagaca acgatcgaag accttgacag cgacctcagc
     6061 aaagaggaag ctaaggttca aaagcaatat tatctcgaga gtggcttcgg cattgatgag
     6121 atgatgcgca gcgggactgt gttgtataat cccgaataca atgactacct caaccgcatt
     6181 gcagatgaat tgctgaaaaa cgaccctgca ttacgtggtg atgtccgttt ttacatactt
     6241 cgttcaccgg tagtgaatgc attcgccggt gccggtggaa atatttttat cagcatggga
     6301 cttattgcca tgttggataa tgaagctgaa ttggccttta tcatggggca tgaaatcggt
     6361 cacattgcta aggatcatgg attagactat tttgttactg caagcgatat agataagaaa
     6421 tctaccgaca atgcattatt caaaaagtca tcttccttcg acccaacttc tattgctaaa
     6481 aacatgtatt cacaagcatt ggaaacagag gctgatgatt atggtatcga tattttaagg
     6541 aaatctaaat actgggcaga cacaaatgcc ttgaatgccg ctttcgatgt cttgaaatat
     6601 gcatatctgc catatgaaaa cgaggccttc ccaatcagtt tttttgaagg agaacattac
     6661 aagatcagca ataatctcaa attggattca ataaaaccaa ttacgggcac tccggaatta
     6721 ttaacaaaaa aagaagcctt aaaaagcaca cacccaagta ttggcgagcg cagaaaaact
     6781 gtgaatcaaa aattaggcaa cctcacaaat gagaaccgtc aggcatatat tgtttcagaa
     6841 gcagatttca gaaaattgcg caatgccgct cgttacgaat taccgatgtt ttatttgcac
     6901 aatgagtttt ttcaagatgc catttatttg agttacctag ggcttcaaaa ggatcccggc
     6961 aataagtatt tggaaaaaat catcggaaaa tcattaacgg catattcaaa attcagaaac
     7021 agtaaggagg atgaagttta tgccgaaaaa gctcggtttg aagattacga aggcgaacaa
     7081 caacagcttt actacctgtt atgggccata aatgatgccg aattgaatac tatggcattg
     7141 ttgtacaact tcaaattgca tttaaaatat ccggaagacc ccgaaattat tcctcaatgt
     7201 caggccttgg taaatgatat ggtcttttat catttcaatg atgaagaaga ttttttaatg
     7261 ggtaaaaaca tcagcaccga tagcctttat ttattatctg accaggccaa tccaaagtta
     7321 aaagttgcac caccaagttc aacttctaag gtgaaaacac gaaaagcttc tgtaagaagt
     7381 aaacggacta ctgccgctaa agttaaaatc gataaaaaca aaaagcaatt gttatatgct
     7441 ttcaatgatt actgggaaaa taaagatttc agaagactct ggaacgaggc agtagtagaa
     7501 cgcgacaaac gcgaagcaga ggctaaagac ttgcgtaaac aaggatataa aatcaaagat
     7561 acaggcgata agcgcgatta cttttttggt caaaaacttg gcgtagaaaa agtaattgtt
     7621 gtaaatccgt tttacaaacg catagatctt cgaaaagaaa atgccgttga atatgtatcc
     7681 agtgaagaag gagaattaaa ttatctggaa gttttgaaaa caaatgctgc attagtggat
     7741 atacaattag aaattcttga tccgcttaca ttgcaggcca ccgacgttga aaaattcaat
     7801 gatatgaatg agctcaacga ttggttttct gaacagctgg atttcggaag catcaacttg
     7861 ccgggatata atcaggcagt aattgattcg atagccacca aatacggaac cgattatttc
     7921 ttatggacag gaattgtatc cttgcgtgac aagcaaaatt tactaggtcc gatactgtat
     7981 attgcgttat caccggcatt tattccacta ttgccttatg gtatttatga gctagtgaca
     8041 ccggaatatg aatttttcta tttgagttta ttatatgatg taaaaacaca tgaaggcaat
     8101 gtgttgaagt ttttgtttct gaaaaataac gactcccgtg ccattttaaa ttcgcatact
     8161 tacgagatgc tgaatcagat agctgcaaaa cccaataaca actaaacccc tgaactgaca
     8221 tgaaaaaatt atgcatcgca atattgctga cagccggatg ttatcaactt ggattgtcac
     8281 aagctgtagg gtatcaaggc aaaaaattca ttgttgaagc gggattttca ccggcaacca
     8341 atttgctgtt acgttacctt gattacagta tggtaaatga cgccattccg gaaggcgaac
     8401 gcgctcctac agaatttaaa atcattcccc gagtaagctt tgaggctgta atttttaata
     8461 gtgggagttt cttcgtaagg gcaaatccat ataattacag ttctactcta tcctatctgg
     8521 acaaaaacta tgatgaagtg catctcatgc agctcgatac taagggcttt atgttttcaa
     8581 taggatataa atcctacaac acaccaacac ctgcaccact gggttcatat tggggattta
     8641 atgttacccg ttataatttc aattcgtcat acgaaaatag tcctgatgat tcccaaggtc
     8701 ttccaaatga aatttccgat tacctgatag cagaatcagg ttgttggggg ctttttgtca
     8761 gttatggggc gaaaaatatc ttttgggata agatgacatt ggatatttct ttagaaggtg
     8821 gttacttttt taacaaccct gaagttggct tgactatgga tgataatgcc tttggaaacg
     8881 atttcgaacc cattcatctt gaatatcctc aatctatcaa tctgacaaat accagggcct
     8941 tctttttcat aatgcccact attcagcttg gatatttatt ctgattcgca aaggtttacg
     9001 taattttgca gtccgcttgg aatctccggg cggacttctt tttcgggccc cgtagctcag
     9061 gggatagagc aactgccttc taagcagtgg gtcactggtt cgaatccagt cggggtcaca
     9121 cttccacttt caggtcgaaa attaagaaat tgcagcttgc acgtatctgc cggatgcatt
     9181 ttacggctgt cagctttaca aggctataat aaggtttata ctacgaaacc aatgtcaccg
     9241 tccattgagt ttggctagct cggtaacgat attattgttg atttcatcca ggctgttatc
     9301 tattggttca aatgcccgtc ctgttacctt ttcgtataat tctatatacc ggtctgaaat
     9361 agttttgatc caggcatccg acatttcagg aacctgttga ccttcttttc ccataaagcc
     9421 attttcaatc agccattcgc gaacaaactc ttttgacagt tgcttttgat gttcaccacg
     9481 ttcttgcctg ccggcatagc cttcaaggta aaaatatcga gatgaatcgg gggtatgaat
     9541 ttcatccatt aagacaacct caccattcaa tatcccgaat tcatatttgg tatctactaa
     9601 tatcaaccct tgttttgctg ccatttctgt accgcgagca aacaatgctc tggtatattt
     9661 ttccatttga acataatcag cttcacttac taatccctgg cgaaggattt cttctctgga
     9721 tatatcttca tcgtggcctg ctgccgcttt tgttgttggt gtgataattg gttcagggaa
     9781 tttgtcgttt tctttcatcc cttcaggcat atcaacgcca caaatagttc gttttccact
     9841 tttatattct cgtgctgcat gaccgcttaa ataacctcga attaccattt ccactttcac
     9901 aggttcacat ttatgcccta ttgccacacg tgcatcagga gtagaaatca accagtttgg
     9961 tgctatatcg ctagtagcgc caagaaaata tgctgcaatc tgattcaaaa cctgcccttt
    10021 gaaaggtata gggcgtggta aaatcacatc aaacgcagat atcctatcgc tggctaccat
    10081 caccagtaaa ttgtcgctta tggtataaac atcccgcact tttccgtgat aaacggccgt
    10141 agcgccgggc aatttgaagt cagtactcgt tattggcata caatattatt gctttgcaaa
    10201 aatacgactt cattcatgaa tgcgatatgc atgtaattgt gaaccgactg cagtaataag
    10261 tattgcatca ccattcaagg taagatcact aatggctgaa agcgcagatc ccggcatagg
    10321 aaatcccgaa agcaattgcc cttgtttgtc gatgagtgca agctctccgg tagtggttgt
    10381 gtatccataa aaccatctgt ccggcccttt taatactact aattgatcta ccatgccctt
    10441 gggttcagaa acaacggtag gttcacctga aagaccggca gctttgataa atccattttg
    10501 cagcatgacc acacccatgg agctatcggc aaatcccggt aacaagcgac attgtgtact
    10561 ttcggaagcg agtttggaat tattaccgtt aaaatcgcta tcaacccaaa atacggtgcg
    10621 catggtatcc agcatgaaaa cagttgaatc catcaccatt aattccttca ttaagggtga
    10681 aggaatcttc tgcgtataca aagagctgcc atttgctttg aaaaatgcca atgatcgagc
    10741 atctacatat cccgggacac cgcccttcga tgttggtaaa ataaacattg gcagattaat
    10801 gcctccgcta cttttcagtg ggttccaacc tgctattggt ttaccatctt tataatagcc
    10861 atacacattt ttattttcgc atgctacaaa cattcggtaa tcactttttc ctgacattgc
    10921 aagtgcaaca ggattggata ttctggtagt cagggttatt ggaaatcctt ctaccgcatc
    10981 accgcgcaca tcgataagaa atatcgattc accggtatta aacaggaatt gtgttttatc
    11041 atttccataa aaatcaactt catgtacttc accaagaatt gcagaaggca agggtctttt
    11101 ccaaaccaca tcgccatttc ctgagatcat gtataattga tgtgcggtat cttgaacaag
    11161 gatatttctt attccggtaa tatcgttcat cacagcaaat ggacctgcct ccacgggtgc
    11221 gtcaatttga gtagtccaca acattcctgc tcctcgttca gcagttgccg tataggaaat
    11281 aaatccattg aacatataca tgtctttatg ctcagtgaat tgcaactcaa tgcgtggaaa
    11341 caattgcaac aatccgaaat tttttgcgat tgaatcaatt tgtttatcac tcaccatttg
    11401 tctgatgagt tgctcagaag aggctagatg taaaaagatt gaggtattaa atgatgcgga
    11461 aacatttcct ttaaatgatt gatattctgc ttgctcagtc aatgtgcctt tattttcaac
    11521 tgcatcgatc atttgcagta attgttgcct atcgcatgca agcacaaggt caccttcctc
    11581 ccaagttgca aagcaagtat cgggaacggg caaatgcagg atggcttgca acacatcccc
    11641 ttgtgccatt gcaaaaatgg gatgattgcg ataaagggct gtatcctgta tactgatttt
    11701 atttattttc gaaactgcca attgcttgtc ttgcgcgggg atgatagcaa atgcccttcg
    11761 catgtaattt ttgtccaatg attctgatat gccgatgatc aattgtgaac ccatccatcc
    11821 cgaccaatta tcaaaaaacg aacgattaac ttcttcgccg gtcatgccgg tggataagcg
    11881 ctctgccagt tgctcagttt gaattctaaa cagcacagcc gtgtttgcag gaacagcggg
    11941 atgcatatcg ctgctaaatg cacctgaata ccgggagatt gtagcattat ctgcggcaca
    12001 gaatccattt aatgaaattc ctttttcagt attggtgagt gtacatccta tccaatcggc
    12061 aaatttcgca acctgcataa ccgaggcata ttgctgatta cctgtgaatg aagctaaaaa
    12121 ttctccaaag cgtggcaggt gtatgtacaa ggtgtatggc tgattatcgg gaagttcctg
    12181 cttcacctta ctaaatgatg attgttcgct taccgggtca ccacttttta attgatgcaa
    12241 agaattttct accaaaacag atgccgtact ataaatgagc atgccgtttg ttaccacaaa
    12301 agatagtgtg atattcaaat ctgcatagtg caattcatac actgtttctt tttcaaaagt
    12361 atgtgttctg acagttggtt tttgcccgtt gagttccggc aataacgctt tggatttaaa
    12421 tcgtttggcg ttgttaagat cggacagaaa taaataatcg acacgacctt caccggtaga
    12481 aaatgcacct gccaatccat gtccgtttgt aatatcagct agcaaatcgg gatcacttgc
    12541 aagaatggcg cgataaaggg cataatcatt atagagcttg ccaaacaatt gcaaatcagc
    12601 aagtgattgc atggcagggt catgctgaat aaattcatta aatgattctg atttattgat
    12661 atcgaaaaaa catatcacgt catcaggcat agcattcatt acgccgctat agcgtttatg
    12721 aataaaaaaa taccagccta aagtgagtag gatgatcaca gtggcgatag atgagatgat
    12781 aatgattcgt ttgcgcatga ggtcttgatt acctttgcaa aggtagggta acaaataccc
    12841 gcagcctatt cacctttaca catcatgata aaatatgaac gataaaagca tcattcggat
    12901 agcagcctgg agcggcgcgt tatctgtagc attgggcgca ttcggagcac atggactcga
    12961 atctatggct gaatcgggca aaatcgacaa tgccgacttg cacacattcg aaactgctgt
    13021 gcgatatcag ttttatcaca cttttgccct gatggcagta gggttgttac acaattacct
    13081 ctcggagaaa tcgatgcgta ggattgcatg gctgttcatt gcaggaatac ttattttttc
    13141 agggagttta tatcttctca ccttttcacc ggttctcttt ggcgagagaa tgggttggtt
    13201 tggagcaata actccacttg gcggattgag ttttattgcc gggtggatca tgcttgcctt
    13261 gcaatttcgc aaatcccggt aaacatattt cgaggcacat cctaaaagaa aaaaggccat
    13321 cctgagcgga cggccttttt caatatagct ggttatgctg ttatttgcac ttaaatttca
    13381 tcttagttgt atcaaattcg catccacctt tttcagcaac ttttacacct actatgaggt
    13441 tttgagtact ggtaagattg atttttgtat caacgtttgt caatgtaata tcgcctaact
    13501 gcaaggtttt aatggtaaac acatcacctt ttacggttgt gccgtttccg aggtctgttg
    13561 gtttatcatt tttgatatct gcagcagtga tggcacctga ttcccaaagt tgaataaagt
    13621 aatctttacc aaccatggca atggtgatgc ttggactcat gttgaggata tacttagttt
    13681 gatttttgcc gttaacagca gcatttcctt caaacatatt acccttttta gtcattgttg
    13741 gatctaaagt tgaagccgca gttgttgtgg tagtagttcc tttattacct tttgtgcctg
    13801 tagttccagt tttaggagtt tcagtgccac ctgtatcagg agtatctgtt tcatcaactt
    13861 gctgaaggtc ttgaagacgc ttgttacgtt tttcttcctc ttcacgcttt cttgcttcgt
    13921 tcgcttctat cgttgcttca tcataagtga tgatagcgtt tggaattaca cctacgatag
    13981 tgaactctgt gcggcgatta agctgatgca gatattcata gtcatcacct ttgaatttat
    14041 tgatgaaggc ttcagttaat acagttccgt ttggaatcgt tgttccgcct ggaactttaa
    14101 attcactagt gatggttttt ggtgttgttt caccatatcc tttagcaatc agtcggtcag
    14161 ggttgatacc gcgagatatc aggtaagacc taacagtatc tgcgcgatgt tctgaaagga
    14221 tgcggttttt ctcttcagta tcgcgagagt cagtgtgcga accaatttca acttgtaatt
    14281 gcggactcaa ctgaaggata ttatataatg aatcgagtgc tttaaatgat tcaggacgaa
    14341 ggttccaagt acccgttgta tagtaaatat ttttaacctc aattggtttc atttctataa
    14401 ttggttccat cataatatca acaaagaggg tatcactttc tttcaaacca acagtgcttg
    14461 ttgtagcaat accgttttta tatttttctt tgtgcgctac aatctcgaat ttatcttgag
    14521 cagttccaac ataaaaatct tcatatgtat ttgctgtttt ggtaacagag ctatcgcgtt
    14581 taacacctga ttggtttaag tctacggacg caccataaat aggttcattc gtattcatgt
    14641 cgatagctcg agccatgatt acaaattccg gcggatattt atatgcatag atatcatcgc
    14701 agcaagtttc actttttaca gagaaaccac ctttgcggtt tgatacgaaa aatccttttt
    14761 tgcctgtagc gtctgttgcg aagtagaaat catcaacaga gctattgaat ggcaagccca
    14821 tgttcatagg cgcttcaaaa gttttagcac caccttttgt tttatagata tcgaaaccac
    14881 cgatgttgat ttgtccgtta gaactgaaat agaaagtttc ggtagtagca ttatagaatg
    14941 gggtaatctc atctccaaaa gtattcactt tggttccgca gttcattggg tttgaatagc
    15001 tctttccttt attttgaatt tctgaatacc agatatccat tccaccgcga ccacctgtgc
    15061 gattagatga gaagtagaga tattcctttc ctttaaaagt accaactgta ggatgcgtag
    15121 aagtaaatcc tgattcattt acctgcgata cttctttacc atttgaccat tcgcctgctt
    15181 caaattcact cacaaagatt ttacatacca tcaccaatga atcgttagga ttacattcag
    15241 tatagtaaaa acgtttgttg tcgagcgaat aagcgccgtt tccggtatgt gctgctccct
    15301 tattaaaagg tgctgacatt tctttcgatt tcgcccagct ttcgccttcc attttagatt
    15361 cgaagatgcg cgctttgagt tctgttttct tttgtgaacc catatctatc acatcttcgg
    15421 aaaccaatga tgcgtaagta agatcgccat cagggttata acgtggtgaa aggtctgtat
    15481 aattattatt tacattgctt ccaaggtgtt caacatttac atccgcaggt tttttgccga
    15541 gctcaagtgc aaggtcgcaa cctttatctt cattttttgc ctgacgtttc agcgcagttc
    15601 ctgttgttcc tttgaggttt gaatttttgg aaacggcttt gaaagcttct ttagcttcgg
    15661 catattttcc attgtatttc aagctcatgg cataataaaa tcctgcctca ggataggcac
    15721 caccttttcc tttgccggca tccattgcag ttttgtacca tttttctgct tctttatagt
    15781 tgcgagccaa gtaatatgca cgagctaact tgtaggcaga ttcttcagaa gcttcatcgt
    15841 catatgcttt tttgtataca tcttgcgcat tatagtagct tcctgccgac atcaacgcgt
    15901 cgccatacat caatttgcgg gtaacattca tgtcgtccac atccggatca acaggttgcg
    15961 caaatgcttt tgatgcaaac aatacgatcg taagaattgc gaatattttt ttcatatgct
    16021 atttctcttt aaggaggtag taatcaaatt aattgtgctg ataatcaata acgcggacaa
    16081 ggcattacat aattttttgg tattacaggt aaaatacaac cggtatacac caatgaaatt
    16141 tcgaaaccgc ctttaccacc gcttgcatct tgcaatgttg aagtattgat atcgtagctt
    16201 gcgccgaagc ggaagttttt ataatccata cctactgatg cgatcactgc atcgtcgagg
    16261 cgatatgctc caccgaggaa gaatgttgca gggaagtttg cactgttaag gaagtatcca
    16321 aaatcactac caacaatgat ttcttttgca cctgactgcg actgatacaa tactgatggg
    16381 atgataccat attttccatc gatagtaata ccagctttca ggtgaccgga gatacgcata
    16441 cccaatttgt tttcttcatc ttgcaggaat gtttccttag gagaaatcaa gttgaataga
    16501 ccaacaccac cggtgatgtt gaatgttttt gaaacagaat tatggtaggc tatacctgcc
    16561 cccatattaa atctgctgat attttctccg atattttcga gtggatcaat tgccgtatta
    16621 aattcatcac catcccactg atttggccat ttgagtgcat taaaatcaag tgatttatgc
    16681 atcaaacctg cttgaacacc aattgaaagg ttatgatttg tttgaccacc aagtcccaat
    16741 acaaaagaaa cgttacccat tgcagagagg ttggttaaat ctccatcgcc ggatacgtcg
    16801 ttgtataaaa gcagaccgcc ggcgagtttg ctggcgccgc cgatatcttt gataagtggc
    16861 atgtcgccaa acacttcata ggtcacataa ggagtagtaa cgctgttcca ctggttgcgg
    16921 taagtagcac ctatacgcca gtcacaacta ttcacacctg tgagggcagg gttcaacgcc
    16981 agaggtgagg cgttgtattg tgaaaagtga atgtcctgag cctgaagcac tcccacagcc
    17041 atgcaagctg ccaaacacgc gattttgtaa agcttcatca ttggttattg tttaaaattt
    17101 tcagagtaat gttatgttgg ttatcagttt ttgcttgttt ttggagttat cggctgtata
    17161 ctaatgccta catcccccta ccccaacggg ttaaaaatac taaattgtcc ttaatctgca
    17221 agcattttgg cttgttagcg atcaattgga actcaaatta tcttaccagg gtgaaattac
    17281 cttgttgtac aaagctttca ccattcatat ccttataatt cagcacatat acatagcttc
    17341 ccaaaggctg ttctgagccc tcaaaagtgc cattccatgc tgctgcataa ttatcggttt
    17401 caaaaaccag ttgcccccat cggttgtaaa cctgcaataa ccaggtagcc acctgacctc
    17461 tcacaataat ggtaaatcca tcgttcacac catcagaatt tggtgaaaac gctgtgggaa
    17521 cacctaccat agcatcagag cttactgtaa ccgtcacttg atcggtaaac tcacatccta
    17581 tagctgtagt aaccgtaact gtatacgttg tggtaaagtc aggtgaagca gttgggtttt
    17641 caatatctgt ttcactcaaa cctgtagaag gcgaccagct gtactccacc ccgccatcag
    17701 cgtcaaggct ggcaaattcc cctaagtata tagtagtatc cgcgcctgca tcagctgtcg
    17761 gttcttgtat ctctacagta gtttcagcgg tatttacaca tgaggccagt gttcctgtaa
    17821 cggtataagt ggtattctca tccgggaaaa ccgtagtagt agcttcagtc gggtcggtaa
    17881 ctgtttcagc aggcgaccat gaatagctgt cggcaccgga agctgtcagc gtggcttctg
    17941 ttcccggaca gatcaatgta tcggctgaaa ccagcaatgg cagggcatcg tagaccagaa
    18001 ctacaagact gtcgccaaca cctgcaacgc aacccaggtc gagtgtgatg ataatagttt
    18061 caaccccttc gtctatgcca tcttcttcag ctacaatagg tatttcgatg agggtatcgc
    18121 cgggtaagaa ttccaacacg ttgttgaaag tggtataatc actccccatc agggcagttc
    18181 catcaacgtt caatgttacg gtaaaggtat caaccggtgc aaatgacagg ctgaaagtga
    18241 tcataccttc attacagcct tcaatgatat aagggtaacc ttcgatatca gtattatatt
    18301 ctacggtaac acccgggctg gtaagtgagc ctgcttcaat gaatacacct gagtctaggg
    18361 catcatctaa gtcatcggca atggccattt tcaggtgata tgtttcacaa gggaatacag
    18421 cgtgttttgc ttccaaaacg gttgtaaatc catcgtattg tatgtaatag tcatctgagt
    18481 taaaaggagc attaaatccg gtgccattaa tattataata ttcgctgttc acggcatcgt
    18541 tcacattatt aatactcacg gttgtagccg tgcccggaat caaggccata ttatcagttc
    18601 ctgcaatacc gggaccgctg atatagaagg caaatacatc gttgaaagaa ccaacgtatt
    18661 ccaggtattc ttcagatcca aaggaatagt tgaaacgaat ggtatctgac gttgcggtga
    18721 aatcgaattc cagaacgcag gcgtcattag ttcccaacac ccccggaatc atatccaaat
    18781 caggatcacc gggcattccg aggctgctcg attggcttgg tgagttgttt ggtccaactg
    18841 catttccgac acttccgctg gtaagcagga taccgttggg tatgcctaca ttacaatcaa
    18901 cacaatcaaa gtaaccgtaa gcgccgccgg ggcagtcgat agtgatgtca gacaccgtaa
    18961 caccttcacc caaaagcgta gaaacaagct cttcggcagt ataagtatca tcaacattca
    19021 attgcgcata ggtgttgagc gggagggtta ggaaaggcaa caccgacaac gcacagagga
    19081 ggttgcgtag aacagttttc atcaaaaact aatttaaatg tgacactaaa atacaatcaa
    19141 acttacaaac aaaataatat tcaacctaaa agcttgaaca aataagctca tgccaatgat
    19201 ttttaccgga tcatgacctg gcaaaatgct gtgtgtgagt gaaggagctt gatgtcaatg
    19261 gctcagacga cgtcttttct ctacttgaac gagcaccaaa gccataacaa gtccgaaaat
    19321 tacgaaaatt attgaaatga aggttgcctg ggtagccctg ataccggcaa actccagcac
    19381 attattgacc ctgatttttt cgataaaaaa cctttctatg ccaacaaaaa tgacataaat
    19441 ggccaaaatc atacccggaa cggttacgcg gcgacggagc caccacagca tgccgaagat
    19501 cattagtgac ataatcgttt catatatcgg agtcggaaaa acagatgccg gaagttgtcc
    19561 gcaatagtcg ccctgacatt tagcaatgta catgccctct ttattgacat tataggcata
    19621 attctgtgct acgaggctgc gtggtaaaaa accgggagct ttaacataaa ccgatttcac
    19681 attagataat gaatcaaatt ctcgaatgta gtatgcagcg tgtatttgga ttacagaatc
    19741 aatttcacct ttttcggctt cacgaagggt gccattttcc tgtgagatat atgccgaatt
    19801 gattattccc caatcgccat cacctgaaac ctgacatcca atgcgaccaa ttccgtaagc
    19861 cagaattaat gcaggagcaa cagcgtcgcc aaggtgcaaa acatttattt tttttcgaat
    19921 tgcataataa ataatcgcag ccgcaccaag aataagtccg ccgtaaatag tcagcccgct
    19981 gaagaaattt ttaaaaggat tttgcaaaaa ctcagaccag ccgttttctt cttcaaaatt
    20041 agaaaacatt ttcgctccaa ggattccaaa aattgcagcc aacatcacaa aatcaccgag
    20101 tcgtgcagat ggatatagca gtaatgtttt ttcctgtggt tgaggtaatt gttccttctt
    20161 tttttccctg tatttactgt atgctaataa agcggcgccg agcaatccta ccaggatatt
    20221 cccttcacca ctaaacataa aactttgcgg atcatcatta aatgtttgcc aattaaaaat
    20281 catgcctccg attttaaatc cgaggataaa tccgaacacc gcattgctga tcaactcaaa
    20341 agcagatgcc ggttctccaa ccattacttt caccttttca gcgtgaaaaa tccctgcctt
    20401 ttccttgcgt ctgagttccc tgtataggat atagcctgct gccatgaacg ccagtcccac
    20461 aaagaatccg aagctaaaaa ccggcaaccg catattggtt ccgaacactg cattaaagaa
    20521 ttcaccaagg ttggcatagg aacgcagtaa tatcataatt caggagcttg aaggtgatga
    20581 aattgatgac tattttcagt ttgatctatt gctattgcct gcatgtgtcc ttcagcgtca
    20641 atttcgccaa gtgcaacatg tactatttca tttaccagca atggatcata ttgtgtttta
    20701 acacgaatat agttgtctgt aaacccaaac atccactgcc cgtcggtctc ttcttcaaac
    20761 aaaacaggtc gggttgtatg ttgatgttgc tcatagaatg cacgtcgttt tttttcactc
    20821 aaattgcgca actgtgcagt gcgttcttcg cgaatttgtt gaggcacact atcaggtaat
    20881 tcaatagcta ctgtattagg acgttcactg taggtaaaga catgtaaata actaatatcg
    20941 agtgtttgaa ggaattgaaa ggtatcaata aaatcttcgt gtgtttcgcc gggaaaccct
    21001 gttattacat caacaccaat gcaacaatca ggcatgagtg atttgatcat gccaacgcgt
    21061 tcggcatata gttcacgcac atatcttctg cgcatcaatc gcaaaatttt attgctcccg
    21121 ctttgcagtg gaatatgaaa atgcggaaca aattttttcg aattcgatac ttgtttaata
    21181 atctgttcgt gcaataaatt cggttcgata gatgaaattc tgaatcgttg tatattggta
    21241 tcttccaaac tacaaatcaa ctcataaaac gactcgttgt tttctgttgc gttacgcagc
    21301 ccaaaatcgc caagattcac acctgtcagc accacttccg aaacaccttg attcgctatc
    21361 gtatttgcct gctccactac cctgctgatg atattgcttc tgctggcacc ccgtgctaat
    21421 gggatagtac aaaatgagca cgaatagtca catccatctt gtactttcaa aaaagtacgc
    21481 gtgcgatcgc caaacgacca tgcagcgtga aatccggcca cgtcttccac ttttcctgcc
    21541 attactgcgc cgctgccgct tgattgcttt tcgggtaatg cggcaatatg ctctaccagg
    21601 ttaaattttt ctgccgcacc taataccaag tcaacacccg gaatttcggc aatttcctgc
    21661 ggtttgagtt gggcataaca tccgactatg acaatacgcg cctgaggtga ctgacgtagc
    21721 gcttgtttta ctaccttttt acatttttta tcagcatgat cggtaacaga acaagtgttt
    21781 atgacataaa tatcggcagc ctcatggaat ccttttacca tatagccggc atcctgcatc
    21841 ttcctgccca aggtggaaga ttcggaaaag ttgagtttac aacccagtgt atataacgct
    21901 actgaaggca ttgtgacagg gcgacaaagt tactgatttc tacccttagc atccatttta
    21961 taaaatttaa tatatttgag taaaatctct ctatgaaacg tataattccc cttatagcgt
    22021 ttttgggtat tttaatggat tcccatgctc aactgccctt cacaaccagt tcactttacg
    22081 ccctaggcgc cgcctacctt gatcggcctt atatccgaga tatggccgat atcgacagtg
    22141 atggcgatat ggacctggta ggttatatag ccagagatgg caacccaacc gttttagcat
    22201 ggttggagaa tgagggaaca ccgccattcg tgcaacatga tatcgccgca actgaatatg
    22261 gatattacat tcacactact gattttgata tggatggcga catggatatt ctggcaccta
    22321 tccaacttga tggcacggtc ttatattttt atgaaaatca tggcgatgga gattttgtgc
    22381 ctcacgaatt gacatattta tcagggtact atcgaaattt tgcggtttca gaccttgatg
    22441 gcgatctaga tcctgacatt ttaaggatag caccaagtat tggaggtgta atgtttgatg
    22501 taacatggct tgaaaacgac ggtgatggaa atgtgaccag tcatatcatt gacaatatca
    22561 acacgccgga gtctttcggt gccggtgatc ttgatgatga cggtgatatg gattttgtat
    22621 taggcactgc taatcgtgtt tattggtatg aaaaaattgt agccggttac acgcgacatc
    22681 tcgtaataaa ttcaggtgat attttcttta ataaaatttt tattgaggat atggatcttg
    22741 atggcgacaa ggatattatc agtgtttcag actacatcat aaagcaatat gaaaatgatg
    22801 gttccgaaaa tttcaccaca tctgtagcat atgaccctcc cggcggtgca attcttattg
    22861 gacttgggga tatcaatggt gattcctttc ctgacatgtt tggtaaatgg gtaataaccg
    22921 gaaattacca tgtaatcaat ttacttggaa atgcagattt tacgtttaca ttaatggatt
    22981 caattgtgcc gatttgcgga aatgaagcac ttacttatgg cgatctggac agagatggtg
    23041 acacggatta tatcttgtct gaaaattgtg aagtcggacc aataaaggat gtgatgtttt
    23101 gggaattaaa tggttcaaca ccgttaccat gtgcccctgt tgaagtatta aatgtttata
    23161 ccgattcact cggactccag gcaacgtgga gtgacaccta tgcagattat tataaattat
    23221 tcgctaaatg cattgattgc ccggctccat caccaaccat ttcaaaaact gtctacgccc
    23281 ctattggata tctcgatgat atgctgcctt gtcataccta taaagtaaaa gttcgtgcat
    23341 tctgcggagg ctcagtgtat ggcagtcccg tgcttaccgt taccactgat ggcgcagcat
    23401 gcaggcaatc ggaagtggat attgaaagtg tattatctgt atacccaagt ccggcatcac
    23461 atacgctcat gatttcagga attcagattg atgtacgtgc agaaatatac gcgatggatt
    23521 taagcggaaa aacgcttcga ttagaaaatt cagcaaatga aatcgatatt cgcatgctac
    23581 cttcaggttt ctatatttta acaattgaat tgaatggcga tctgtacagg actcaattta
    23641 taaaacaata aaaatgatac ttcagcatgg agctaggcaa ttcgatcttt caaattcaag
    23701 atccatcgcc attccctatt caacaggcga acaggtaaat tgctactatg cacccccgtt
    23761 tcaggtaaca cccgttgtta tgggcacttt catcggcgac atcaatcagg gcagtccggt
    23821 gaattatatg aatgtccaag tgaaccctca tggcaatggc acacatactg aatgtattgc
    23881 gcatatctcc aatatgccgg ttaatatcca aggtgtatta aagaatcatc attttattgc
    23941 ccagattatc agtgttactc ccgacatcat tgaaaacgga gatactgttt tgttgtcgaa
    24001 gaacatcatc ccaattctgg aaaaagatat tgaggccgtg ataattcgaa cattgccaaa
    24061 tgagtcggca aaaactacca aggcatacag cggaacaaat ccgccttacc tggaggcaac
    24121 ggccgcattg tctttcaggg aatcagggat aaaacacatt ttgatagatc tcccgtccct
    24181 cgaccgtgag gaagatgggg gtgcgttggc tgcgcataag gctttttggc actatccgga
    24241 ggcgccgcgc cttgactgca ctataaccga actgatattc gtgcccgatg ccattgcaga
    24301 cggcatttac ctgttgaatt tgcagatagc tccattctgc atggatgcca ccccatccaa
    24361 cccggtggtc tacccaatgt taaagtaaga aatccttcac atattgttac catttcccgc
    24421 cctttgggag attacaattt cggtatcttt gtcggcttta tttttttata tgagaaaact
    24481 tctatccaca ctcacgctga tcgccttgac catcagcgta tccctcgcac aaatgaccat
    24541 tgcagatgca aggttgcaac ctgtaggcac atcagttacc gttaaaggca ttgccctcaa
    24601 cggtcctgag ctgggaagca tccgttatat acaggacgca accggaaata tcgccgtcta
    24661 cagcgccacc cttgttaccg gtgttcaaag aggcgacagt attaccgcta ccggcccatt
    24721 gttcaactat aacagcttac tggagatatc acctgcaagc agtgtaacca tcaatagcac
    24781 cggccacccg ctgcctgcac ctattgatgt tacatcattg tcgggatggg tagaaggcaa
    24841 tgaaggcaaa ctattgcgta tttcaaatgc cacttttgtt gatgcaggaa catttagtac
    24901 cgctggaagt ggaacaaatt atgatgttac cgatgctgca ggcacgtcac agattcgcgt
    24961 agtaactacc accaatatcg atggcacgcc aattccggct gagcctgtat ttattacagg
    25021 catcatgagt caatatgcac ccggtggaac aggaggttat caattattgc ctcgcgatct
    25081 cgctgatatt tcaacaggtg gcaatccgcc ggtaatttct tcagtactca ctcaaacaga
    25141 tattactacc tcatctttta cggtgaattt caacacattg tatgaaggca ataccattat
    25201 ttactatggc acaacgatgt cgctcggatc tacagcatcc tcagctgcca tggtaacaac
    25261 gcatgcatta aacctcaccg gcttaacagc aggaactgtt tactatgtaa aagctgcaag
    25321 tgtaagtgca agtaatgata cttcgtattc tgcaattacg gcaatggcga cggcaagtaa
    25381 ttcgtcggga caaattaagg tatggttcaa taatcctgta gataatagtg tttcaacagg
    25441 gactaatgcc acttttgtaa atagtgcgat ggatgacact gtaatttggt atctcgatca
    25501 tgctaaatat tcactcgaca tagccattta caatattgac aatgtcaact atattatcaa
    25561 cagtataaac gacgcttatg ctcgcggtgt tgatgtgcga gtaatctgcg acaatggcgt
    25621 tgatgatttc aattatggtt tgataaatgt tggcgttggg aaaaagaaaa aatcaccgcc
    25681 agatggatca acaaatgcag atggtgcatt atatggtatt atgcataata agttcattgt
    25741 tgttgatggg atttcagttg atcctgatga cgcctgggtg ctgacagggt caatgaactt
    25801 tacagatcag caaattaaaa tggataagca aaattatctt gcgattcagg atatgagctt
    25861 agcaaaagct tatcgactgg aatttgatga aatgtttgga ggcaaatttg gtcctgacaa
    25921 atcaaataat acgccacatg aatttatgat aggcggaaag cgtgttgaag tgcatttctc
    25981 gccaagtgat gaagttgaaa cacgcatgat cgagcgtatt ggtacggctg attatgatat
    26041 gcattttgca atttattcat tcactcgctt tggcatcagt tacgcaatcg aagatgctgt
    26101 attagatcgc ggtgtttttg ccagtggtat ttgggatgaa acgaatgctg atgacagtac
    26161 ggcaattaat gtttgtgagg atgctatggg cgatcgtttt gaaaaatatt ccggcagcaa
    26221 tttgttgcac cataaatatc tgattataga tccaaattgt cctcagtcag acccaatggt
    26281 atggacaggt tcacacaact ggagcaccag tgccaactca cgcaacgacg agaacaccat
    26341 tgttatacat gatgccactg tagcaaactt attctatcag gaatacatgc aacgttataa
    26401 agatgaaggc gctacagata tggtgaatgc cgcttgcgag gttgcaatca ataattccac
    26461 acaagatgaa ggtgagataa ggttgtatcc gaatcctgca acaaatcaag taactattgc
    26521 tctgggagaa gtaactaatg gcaatatcaa gatatttgat atttcaggca aagtaatgct
    26581 atcaagcgaa gttcacggat tacaatactt taccatcaat attaacgaat tgcctgcagg
    26641 gatgtatttt gtagtttgcg atgttaatgg aggaaataag acaagtaaat taattatacg
    26701 agattaattt ctcataaacc atttgaaaaa acgctccatg aatttcatgg agcgtttttt
    26761 attttattct tttcaaataa ccattcgggt taaatgtgat ataatatttc tccttagatc
    26821 tctcaatttc gaatcgatta tctttcttta aaaacatatc gattgcttca aatggtccag
    26881 ggccatgttc cggctaaaca gggtgaccat taacattact atcttctact aaaagataac
    26941 ttccaacagt caccaaatct gcatagattt tcatttcatt aaaaacatga tctctggaat
    27001 gatcatcatc gagtatgact aaaacagaat tagcatcttt cgctgcagac ttgaatcttt
    27061 ccacaattga ttcctccaga gcagaacctg taaggtattc aattcgagga tgctgaggtt
    27121 tattttcgtg gtcaataata tcaaccgtaa gtattttacc ttttccaatt agatcacaga
    27181 gtgttgcaag gtaataggca ctaccgcctt tgtacgttcc acattctata atcaaatcag
    27241 gcttgacttc atacaaaatt tcctgataca tccatgtatc aaaaggacac ttttttatat
    27301 gagttccaag ccatgttgat tcgccccagg ttttattaaa taaaaaagaa tcatagaata
    27361 gcgaatgaaa gtcatccact acacgatttt ctttttttct actcatatac agccttctgt
    27421 aaatcaggct acggatggct agaatctcag actttaacga cattacttat ctaatctttt
    27481 tattgctaat aattgaaatg ctttacttgg cttatatttt acaaaaggct cggtcaaatc
    27541 gctgataaaa gcccaaaaaa accaaggctt taattttggc ttagcaaatt cgcctctcat
    27601 agatttaata ccatttacgc ccaagacctc atatccaaat gcgccaaatt cttcgggaga
    27661 ccaacctgaa taatgtactt gccatggatt attttcacga tcaccttgag gcaaaaaacc
    27721 atttggtgta tatacgaaga ttttttttct tgccaattta tccatcattt caagcaactt
    27781 caatccatcc tccttgcgga gatgttcaat cagatcaagt gcaacaacta catcaaatga
    27841 tttgggtcca aacttttcta aggcatcaag cacatcaatt tgatgatact cattatgtat
    27901 acccaaggtc ttactttttt ctatactagg tgcaaatgca tcaataccca cacaatataa
    27961 ttttttatta aaggtttgaa ccggtgagaa actgccacaa cctatgtcta gaagatttga
    28021 acaacctttt acttcccgtt ccatgataac atcgtactta ctaaaaaaag acttgtaggt
    28081 atacctataa gcctttctca aaaattttat tattggaacc atactactaa gggcgatact
    28141 ttgaattatc aaaatttcct ctccatccgt caattacagc gttaagaaaa acggcaaaac
    28201 cagcaaattt gccagacaga gcagctctca aagatagata taaaatataa ataagggcgt
    28261 taaatgcgaa tgctagttgg gatttcacta aacgctgctt ttgaaagaag ataaaattcc
    28321 tggtagaata atatactcga aacgggctcc ctcgcaatgc aagtggcaat ttgcttgtat
    28381 ttgctgaggt cgactgccaa ctcaattcaa ggtcatgcac aacagctctc ggctcaatga
    28441 taatctcgcc tccaattttg gctaagcgac ttgtaaagtc aaaatcatca gcatagacaa
    28501 aaaatctttc atccggataa ccaattttat cgataacacc ggatctgaac attaaaccac
    28561 cgtaaggtgc atatggcaaa gttatcgaat ttgtcctact ggagtcgatc ttcatattca
    28621 gcttaggcaa tgatttataa gctaatttct tgaaactgaa tcctaaaaaa tcattcccat
    28681 acgatagcac tttttcaata ttttcaccgg ctacgatccg agttaagtaa ttcctagaag
    28741 gtcgaagtgc gagcaccata atattttgat ggccgtgaag agattcttca gcaaaaagtc
    28801 tttcctttaa gattgcaaac gctccatttt caggaagatt atcgtcatca atcatccata
    28861 caatggcatc agggcaaaca ttgcgaatat attttaatcc ctcgccgtac gcctttgctg
    28921 atccaacatt ttcagtaaaa tcaatccgct tatatgtatt aaagtttaaa accgaagctt
    28981 gggtttccgg gaaatcagtc gagccattat tcacaacaac aacattcagc ccaacatttt
    29041 cacccaatcg ctctaacacc ctacttaaga actgccatcg gttattataa gttacaataa
    29101 gacaatagta ttctgacatc aaataaattg aaatacaaat ttacaatttc ctttgaagaa
    29161 aatacaaaac aataaattct ctaaatacat aattcaacaa gcattttcaa ccccacactt
    29221 ggttacattt taatctgaca taaacagcat aatggtcatt gcgcctattg tttcataatt
    29281 atcgttttgt agacaacacc acccttattt tgtacaagga aataagttcc atccgggtat
    29341 gtggatacat caatttggca acctgtttgt aagggagtcc ctctaaatat ttcctcgcca
    29401 agggtgttaa taatataaaa ctcagtgtcc acttccaatc cttgacaaat tatcgattca
    29461 gttgaaggat taggataaac catcaggcta taagattcag cattattccc gacaattgaa
    29521 tttactatat ttatattcgt gattgaatct actgtgaaaa caaactcaca atcacctgca
    29581 tgctttttct tctttatttt attagctgca ataaacaatg ttgataacga attattccag
    29641 atatcatatg aattgttcaa gcgtttatat agctttgacg aggactcatt ttcaggcaca
    29701 acaactccat ttgaaaatga gtaagaataa tctagcgaat atttagcagt tattccatca
    29761 tttgcgataa atactccgta ataatattct ggaatttcct ggagtccagt cattgaatat
    29821 ggatattgat ctaccctata aaggtgcatg ccaaaaggaa catttgttat attgctcaat
    29881 tttaaatgat ccccaccaat agaatttaaa tccagcgtca cacctaaata gtcgtctgaa
    29941 taaaccaata ccgataaatc tccaatcggc gcgcctgaag tcattttaac tgccgagcca
    30001 atcacatacc catctgtatt tgttgaacta taatctgcaa ctgtgcttga aacataactt
    30061 tcatcaaatc tccaatttcc tattaggccg ggttccaatc cggttaattt ttcacacatt
    30121 ttgtctcgaa tttcactttc agaccttgca atatcccata agcgcacctc atctaaattc
    30181 cctgtaaaat tcacttcagc agccggatgc tcctggcaac cgatataggc cggataagat
    30241 gagtgcaata tagtggtgtt tatggctcct ccatctgtat caactgttga ttgcagcacg
    30301 ccattgaaat aaaaattaat atcatttaca gaaattgcta caactgctat atgggtccag
    30361 gcattcaagg gtgggacccc gattgttttt ttccctcgcc gatcagaaga tccggcacca
    30421 tatccgtcgc caatttcaaa tatcaaatta ccggacgtgt tgaacctcaa ccaaaagcca
    30481 taatagtggc cccctggtcc ataattgtcg cttgccatta ccggtgaata aaccgaaggc
    30541 atccaagcag ttgggtatat ccatgcttca attgtcaatg ggaagttgac tgaattataa
    30601 acatcaccca aatcaactct acttcctact gtgccaggga aatttattgc tatccctgaa
    30661 ccaatttcat tcggttgccc tatacattta tttggcaaaa gagataaaac gaatattaaa
    30721 atcaatgatc ttaaaaaaag tctcaaggtt ttcataggat taaacaaaat ttacaaaata
    30781 atcaataatt tcaagaaagc atgacttaat tatggtacag ctaaaaatta tcaaatctaa
    30841 atgaactttc accagtttaa atttagggca aatatttgta attttggtaa accaatgaaa
    30901 actagtcttc ttcttatatc tgtttgttca ttgttctact ctacgattgg aaagggacaa
    30961 atttgtgact tttatgaaga ttattcagac ccaacccctt ggaactttgt atatgtatat
    31021 ccggg
//
LOCUS       DAOQHY010000015        28116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_724_length_28116_cov_5.910338, whole genome shotgun sequence.
ACCESSION   DAOQHY010000015 DAOQHY010000000
VERSION     DAOQHY010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 28116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28116
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_724_length_28116_cov_5.910338"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /gene="pyrH"
                     /locus_tag="PLL28_02400"
     CDS             <1..525
                     /gene="pyrH"
                     /locus_tag="PLL28_02400"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNF68192.1"
                     /translation="GSGIDRAQGDYMGMLATVINGMALQSALENVGCYTRLLSAIKME
                     QICEPYIRRRAMRHLEKGRIVIFGAGTGNPYFTTDTAASLRAIEVEADVILKGTRVDG
                     VYTADPEKDPTATKYDSISFREAIAKNLNVMDMTAFTLCAENNLPIIVFDMNKEGNLA
                     KLISGESVGTLVKN"
     gene            550..1116
                     /gene="frr"
                     /locus_tag="PLL28_02405"
     CDS             550..1116
                     /gene="frr"
                     /locus_tag="PLL28_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNF68193.1"
                     /translation="MHEEELSMVIDMATEQMQKAIKHLELELSHVRAGRASIVILDGV
                     RIDYYGALTPLNQVANLSTPDARTIAIQPWDKKMLSEIEKAILAANLGLTPMNNGDII
                     RLNFPPLTEERRRDLVKQVKHMGETTKISIRNFRKDGNEEIKKLQKDGLAEDMAKDGE
                     DRIQKLTDKFIAEVDKHLGVKEAEIMTV"
     gene            1344..1703
                     /locus_tag="PLL28_02410"
     CDS             1344..1703
                     /locus_tag="PLL28_02410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNF68194.1"
                     /translation="MFLDLPHKRMDVYIHARKLLLECRKVTDRFPHEEKFVLALQIRR
                     AALSVFLNIAEGSSRFSKAERRRFYEISRSSIVELDAAFDIAETYGYTQAGLLSEIGE
                     LIVRLFQMLSKLINNSQ"
     gene            1995..3395
                     /gene="fumC"
                     /locus_tag="PLL28_02415"
     CDS             1995..3395
                     /gene="fumC"
                     /locus_tag="PLL28_02415"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HNF68195.1"
                     /translation="MSNIRIEKDTMGTVEVPAHVYWGAQTQRSIMNFPIAQDINKMPK
                     EIIAAFAYLKKAAAMTNHQLGVLDKSKADIIATVCDEILEGKLNDQFPLVVWQTGSGT
                     QSNMNVNEVVAYRAHVLLGGSLMDEKKAVHPNDDVNKSQSSNDTFPTAMHIAAYKILL
                     ECTIPGIEKLRNTLAEKSLAFMHIVKIGRTHFMDATPLTLGQEMSGYVSQLDHGLRAI
                     KNSLAHLSELALGGTAVGTGINTPEGYDVLVAKNIAALTGLPFVTAENKFEALAAHDA
                     IVESHGALKTVAVSLMKIANDIRMLSSGPRSGIGEIHIPDNEPGSSIMPGKVNPTQCE
                     ALTMVAAQVMGNDVAINIGGATGHFELNVFKPVMIYNFLHSARLIGDACVSFNDKCAM
                     GIEPIEANIKKHVDNSLMLVTALNTKIGYYKAAEIAQKAHKEHKTLKETAVELGYLTP
                     EQFDEWVVPVQMVGKL"
     gene            complement(3565..4341)
                     /locus_tag="PLL28_02420"
     CDS             complement(3565..4341)
                     /locus_tag="PLL28_02420"
                     /inference="COORDINATES: protein motif:HMM:NF025679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HNF68196.1"
                     /translation="MRKNIISMIVMLPILVVLVATSSCKDDTADRQAHVNIYLTDAPA
                     EYDAVYIDIQAVEITSDMGVETFDVVTPGVYNLLEFNSGIDTLIISEDVSPRTISQIR
                     LILGSNNTVVVDGESHPLETPSAQTSGLKLNVHYTFEAGLVYDLWLDFDAEQSIVEKG
                     NGDYSLKPVIRVYTEALSGAITGVVFPTDAAYYVQAMSATDTAGTYISADGSFLIGGL
                     PEGSYSVNFSAVAGFTDITLPGVSVTTGIITDLGEVTIPL"
     gene            4712..5404
                     /locus_tag="PLL28_02425"
     CDS             4712..5404
                     /locus_tag="PLL28_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68197.1"
                     /translation="MHRVIFVGIGLCKKIIWKSILIYYLSIKTHAKQENKLLVAAVAL
                     TMVFSANRASAQAFYQGVNHLSLGVGFGGYLDYAYTGSGDFSHIPTLFASFDHGTIDD
                     VFGGNIGIGGFVGYSSAKYNYDYLGYHDDSKWTYFAIGARGTYHYILDNENLDLYGGI
                     SIGVLSQSYDYNSNYPWVGFDPNSYYDYNNMVLYSSACVGAKYMFSESLGAFAELGWD
                     IAWLKAGVTLGL"
     gene            complement(5471..5665)
                     /locus_tag="PLL28_02430"
     CDS             complement(5471..5665)
                     /locus_tag="PLL28_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68198.1"
                     /translation="MSDVAWIAVIIIIYIAFIKPMLHGARQSSQQQGGNNIREKKNPA
                     GTSEKTNSKDADYVDYEEIK"
     gene            complement(5737..6111)
                     /locus_tag="PLL28_02435"
     CDS             complement(5737..6111)
                     /locus_tag="PLL28_02435"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNF68199.1"
                     /translation="MFLDLSHKKLPIYLASLTFVRECYKVLDTVNSSERFGIEKQIKR
                     AAVSVHLNIAEGASKRSKIDRRRYYEMARGSLVEVDSGFDILVTLNLIQNTELHTLNN
                     AVNTCFQQLSNLINYLSSEIKS"
     assembly_gap    6269..6368
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6429..9260)
                     /gene="carB"
                     /locus_tag="PLL28_02440"
     CDS             complement(6429..9260)
                     /gene="carB"
                     /locus_tag="PLL28_02440"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNF68200.1"
                     /translation="MPKDTSIKHVLIIGSGPIIIGQACEFDYSGSQASRSLREEGIKV
                     SLINSNPATIMTDPVTADQIYLWPLNVGSIEKILIENIEKGTPIDAVLPTMGGQTALN
                     LAKEAFELGIWVKYSVRMIGVDIEAIDRAENRELFRQLMVELGIGVAKSHVANSFLEG
                     KEFAQEIGFPLVIRPSFTLGGTGGGFVKVKEELDAALMRGLTASPTHEVLVEQAVLGW
                     KEYELELLRDANDNVVIICVVENLDPMGVHTGDSITVAPAMTLSDTCYQHMRNLAIKM
                     MRAMGNFAGGCNVQFAQDPYTENLIAIEINPRVSRSSALASKATGYPIAKIAAKLAIG
                     YNLDELNNQITKSTSAYFEPALDYVVVKMPRWNFDKFPGADDTLGLQMKSVGEVMSIG
                     RSFQEALQKACQSLENNKIGLSGEKGFYKSSQEIIDSLAKPHWDRIFNIKKAMDLGVP
                     VRTIHNQSLIDPWFLMQIQDLVKLEKELRRYQLTNIPKKLFLELKQKGYSDIQIANTV
                     GNCTEEEVYNHRRSLGIKRTYKLVDTCAAEFEAKTPYYYSTFEDENESIVSDRKKVIV
                     LGSGPNRIGQGIEFDYCCVHGVLAIRESGYEAIMVNCNPETVSTDFDIADKLYFEPVF
                     WEHLWELIEYEKPEGVIVQLGGQTALKLAKQLHEKGIKIIGTSYNDMDIAEDRGRFSD
                     LLKDLGIPYPEYGAATTVEEALEVAHRIGYPVLVRPSYVLGGQRMRIVINDEELERSV
                     IGILKHFPDNRILIDHFLDRAEEAEIDAICDGENVHIMGIMEHIEPAGIHSGDSSAVL
                     PSFNLSERVLEQMKAYTEKIAFALNIRGLINIQFAIKNEQVYVIEANPRASRTTPFIA
                     KAYQIPYLNVATKVMLGVKKLSEFTFDKKLQGYAIKEPVFSFEKFPNVNKELGPEMKS
                     TGEAIRFIADLQDPFFREMYSKKSMFLSR"
     gene            9491..10303
                     /locus_tag="PLL28_02445"
     CDS             9491..10303
                     /locus_tag="PLL28_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF68201.1"
                     /translation="MTAAGDFQFGDAVFHYTRFGNGTEVMLAFHGFSEPGTSFLPLEP
                     ALGLRYTIYAFDLPWHGETQWPEGRIFTPADLLGLVRGFLDYLGASKFAVLGFSMGGK
                     CALYITKHMPDRLNALWLLASDGIQTNRIYNVAVYPAWGRKLFKTTIRHPSWFFGMVN
                     LATRLRLITPWLKKFTNNHMATREKRQRLYDTWISMAGFNPDIELVKKQLNTFHIPAL
                     LFFGRRDEVIPYTVGEQFAEGVALAKLTVLERGHYFIDEKINPMLEEAMHFI"
     gene            10345..11469
                     /locus_tag="PLL28_02450"
     CDS             10345..11469
                     /locus_tag="PLL28_02450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF68202.1"
                     /translation="MLFITIGTILLIAGYVSLVAFFLYGWWKLKEFNGTATKKHVFIT
                     VIVPVRNEEKNIVHLLRDLKEQTYPADKYEVIIVDDHSTDQTTQKVMQAKMDNVRIIS
                     LGDAGTVRMNKKAGIQHAVSSAKGELIITTDADCHVGNKWISTIAAYYTEHEPVMIAG
                     LVNYFPDATFFGKFQTLDFLSLVGIGAACIQNGFYNLCNGANLAYTKAAFEAVNGYEG
                     VDHIPSGDDMMLMHKMARKFPGKIASLKNKDSIVYTHTETDVVSFWHQRVRWTSKSTH
                     YEDKRITLILSFAYLFNLLIVFNMVIGFFVPGMLKLSMWMFLVKICIDTLFTYAVSRF
                     FRRENILWLFLPIQALHVLYIIIIAPAGVFGKYQWKGRRH"
     gene            11474..12865
                     /locus_tag="PLL28_02455"
     CDS             11474..12865
                     /locus_tag="PLL28_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF68203.1"
                     /translation="MKFKRKLLSILIFVAASGAIAVLWKLLDTDIDWFVAIYNSITAV
                     MVIYVGTLMLFIYPIAFLRKYWEKKKSQIDLNAKPKYKVYDRLMRNTNALAGGLFILW
                     VALAAIFAHIIIPDKSPYAGDQMNEIAYKEPGFSVKVLLVPADRHVSSTGVIDWFLNG
                     KTSSFKQIPINDAWLVKGDSLYVSRYLGDNMVGIPMVFATEFITGKKSVVAEQSQIIE
                     NEFIETRHFWLGTDDLGRDYLSRIILGARVSLSVGIIAVLIALFIGIPLGALAGFYKQ
                     YPPKLRFGKKRAVKVPVDNSVMWMINIVWSIPTLLLVFPIVFAFGQQYYTIFIAVGIT
                     MWVDIARMVRGQVMQVREMEFVQAARTFGFSDTRTIWRHVLPNITGPVIVVTAANFAY
                     AILTEAGLSFLGIGVQPPAPSWGLMIAKYKDNLITDPYLPLIPGFAITLLVLAFFMVG
                     NGLRDALDIKSRL"
     gene            13004..14254
                     /locus_tag="PLL28_02460"
     CDS             13004..14254
                     /locus_tag="PLL28_02460"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNF68204.1"
                     /translation="MAIAGLNVMEMLRNQLKLKQLEIDSLLEITKSINANKSASALFE
                     LYHRTLRDQIGVENLLLFSNMGGDWEAMIGDKLISEDHPEVMQYLLPYEKLTDLTTVD
                     IPDYLSEYDLLIPVLHKDKPLAYLLMSNPRVESYEPLEDKIKFAQTVTNIVIVAIENK
                     RLFKREVEQQAFKRELELAAQVQSMLIPDTLPHTEQVQMAAIYLPHKNVGGDYYDCLE
                     LNNKEIFFCIADISGKGIAAAILMANFQAQIRELTKRNPPTIEEYMQELNKGVLMSTK
                     GEKFITLFLAKYDRTTGYLKYVNSGHNPPILVNSQGTKLLDKGTTILGMFDTLPSVKM
                     GQLPITEDTAIVCYTDGLTDVVNEFNQVFPLDELIELAKQHYKEPVEVLNKSIIDEIM
                     HFKGHDGEVSDDITVLSVKIHPAN"
     gene            complement(14251..15714)
                     /locus_tag="PLL28_02465"
     CDS             complement(14251..15714)
                     /locus_tag="PLL28_02465"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNF68205.1"
                     /translation="MLKTLEKPVEFSIYWIATLLAIVAIFAGVILEEPLYFAVPVAFL
                     FAYQMIVDFRVIFFLLLIATPFSIEYYSSSGFSTTLPTEPMMIIFMFTFIFFIFLKRE
                     LFDTGFFLHPLTIILYVHFAWMLVTTMFSEDIVISLKYMLAKTWYITTFYFMTSLVIK
                     DIATFKKAFWCLFVPTLALTIYTLINHNKYHFAFSEVNKTMVPFFRNHVNYAVFLALM
                     LPMLFSAYRWYPKNTWQRWIIQFGIVVLIAGIYFSYTRSSWLSVAGATIAFFIIRMNK
                     LVTSAVFAVFGVIIFAIVMLHNNRYLDYAPDYKKTIYHTNFNEHMESTMSLEDVSSAE
                     RIYRWVAAIHMIEDKPYMGFGPGQFYFNYKEYTVNKFETYISKNPERSTVHNYYLQVT
                     TEQGFPGTFVWVVLLIYTMYLGQRLYHRFTNIEYKHISMSITLSIITIIINLALSDLV
                     EADKIGTSFFMFLSLLVSFDLLLRRNPALSAEAPAKD"
     gene            complement(15707..16606)
                     /locus_tag="PLL28_02470"
     CDS             complement(15707..16606)
                     /locus_tag="PLL28_02470"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HNF68206.1"
                     /translation="MEQRNDLIRLLQIIFKWRKPIIITTVVVAIAACIITWFFMPDYF
                     KSTVNFYPSNPIMTDRQVLFSQSTGEIEIDYFGTASDVDRILTIANTSSLIDYVINHF
                     HLMEHYDIDSTKEMARYNCKKEFLKNYSAVETEYGAIEVSVWDKNKDTAMHIANHIVE
                     TIDQHNKDIIMRDKKLIVNTFKNQVAKKELIVTNLTDSIAAQRKAGASAEMMIVLDSK
                     LQSAVDDLNLDRKLLEQNETTLNTDFSTVHITEAAYPALRKDKPVRSLIVIGVTLGTI
                     VFMILLAVITENYRKIKSRIQNA"
     gene            complement(16643..18112)
                     /locus_tag="PLL28_02475"
     CDS             complement(16643..18112)
                     /locus_tag="PLL28_02475"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNF68207.1"
                     /translation="MRKNFITNIGFLLFLNLLVKPFWILGIDRTVQNTLGPEVYGVYF
                     ALFNFSYLFQVVLDFGINNYNNRQVAQDPQTLGRNIMSTLVLKLLLGVVYTIIVFIAA
                     FGVQFSGEQFQILGLLVLMQIALSFYTFLRSNVNALHLFRTDAVLSILDKLTTSILCA
                     WIFWGASGITMSIPLFIWIQIGGFVVAILVAVAVLSRQKLTISNRIDKALIKNIFKGS
                     LPYALLGFLMTAYYRTDGVMLERMLGDKGPYQAGIYASAFRLLDAMSILGFLMAGMLM
                     PMFARMISQNTSVKPLLDLGFQIMLICSISAGIACLIHREDIMQLLYHVHDPSAGSIF
                     GWLMASFICISLTYVYGSLITAHGSIKMLNYISFGGFILNIILNLMLIPEQQALGSAK
                     ATVFAQVLILLAHIAVAHRLFSLGSNLSKWLRSIFYLAAVIGVNLLIAVFNISWTWQW
                     VIGLASGAFLAIPAGIINMTEVRSLKTVMQSAIARRQGS"
     gene            18248..19411
                     /locus_tag="PLL28_02480"
     CDS             18248..19411
                     /locus_tag="PLL28_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNF68208.1"
                     /translation="MLIAVNTRFLIKDKLEGIGWFTYETMKRLTRNHPEHHFLFLFDR
                     DWDPEFIFSDNITPVKVWPPARHPYLWKYWFDTALPRIFKKYKPDLFISTDGMMSLAV
                     NVPTVLVVHDLAYIEFPHHIPRLTYEYLKHFTPKYLSKADAIITVSDYSKKDIIDKYQ
                     IADSKIDVVANGASDNFKPLDEVNRSIVKQKISSGSEYFIYVGSIHPRKNVGMLMKAY
                     DQLRAEGICDHKLVIVGRYAWKSGETYNIYKSMQFIDDVIFTGSMNQEQLTEAIGAAD
                     ALVYPSLFEGFGIPVLEARYAGVPVICSNSSSLPEVGGEHAIYFDPNSAEAITTAMVQ
                     FLKNRAELKRNALESAYEARNIYNWDNSANKIYSIMQATYDNYRANRKYTANT"
     gene            19368..20057
                     /locus_tag="PLL28_02485"
     CDS             19368..20057
                     /locus_tag="PLL28_02485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF68209.1"
                     /translation="MTTIEQIENILQIPDTDKKTPVRVEEAKFIYQFILGHKIHKTLE
                     IGLGYGRSASYIMLATGKPHIAIDPFQSHYGYQAIKNITDAGLKDNFTHIEDFSHFAL
                     PELCHKNEKFDFVFIDGDHKFDGILIDYYFASMLVKTGGYILFHDTWMRSTRLVNKFV
                     EQNRHDFKTHPVSLANLHLIQKIQDNDTRDGMYFREFYTTKSWLKYRLITHLNDHPKG
                     LLSKLKSIFRP"
     gene            20068..21741
                     /locus_tag="PLL28_02490"
     CDS             20068..21741
                     /locus_tag="PLL28_02490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68210.1"
                     /translation="MKKLFWLTALCMLLTQVSTAQIYDIYSVIPTFNTVETDNYCSDS
                     PIAVEVVMTAGAITDDDGFSIRMKSYPGGLSWAGYDGLCPDDYNCAYGFSIDPGDVPD
                     GLYYFEASCKFFAHAGDPDYEQSEVFSLKTITCGNVEDPLNWTSAKVRDNTGMSMADG
                     KIYYVDDNRKVRNFYWTGTNWISDVLNTTAPKAKDGVDIVATVENVYYVGDDNKIKNL
                     YWTGTDWAYTSLVASQPEVRSNSNIAFGDGKIYFVSADNNKVYNLYKVGATWYCSALN
                     NDAPDVRDDSKLATDVGKVYFVSSTPLNASTNKMIYNYYWDGDSWEYAMLNSSAPKAM
                     DGAGPAVCNAKVYYTAEDQKVKRLKWNTATGVWDVNSLLATQKLVDAGEQIAVVDDII
                     FFHNSFDLVYYLKVSGHAWNEYSMSPCNAYSSTSYSDIRDNTTIIPEGTGKVYYVGTD
                     RRLRNFYTVACRSSIEISPVPTEILTAFPNPSNGIIKLMNASTEPLSINTLRVVDLQG
                     KMVYVDTESKLVNANASLDLDLSQIPNGVYFILSDAGDEIISNRIIISH"
     gene            21882..23780
                     /locus_tag="PLL28_02495"
     CDS             21882..23780
                     /locus_tag="PLL28_02495"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68211.1"
                     /translation="MVIRMLFSKIGLYVSALGACHCSIDMAYSQKVDNDHRDQYAPSA
                     VVIMHEIKQDYNPIWYSTEMPAPGSNSYRRHLIDLKDELYRNYMPQQQSNLLREETDI
                     TSPEILSGWEGNLITSYVPNDNDLAISNGGMIVSVINSNIYIYDDAGTGLFVTSLQSF
                     ADTLGILDSKFDPKVMYDPRADKFVLLFLAGFDPEHTHVILAFSQTNDPLGAWNLYSL
                     PGNPKDNNRWTDFPMFALTENELFLTINLIIPDEPWQTGFSETLIWQMSLDKGYAGDT
                     LDAVYYDSIFFEGKPLRNLNPVKGGANTYGPDMFFLSNRNFDPANDTIFIVHVTGTLD
                     DPETELTVDFGRSNIAYGVPPQARQFSTHIFDTNDGRVLGSFYENNQIQFVSNTLDPA
                     TGFCGVYHGVITDPTGAKTITANIIGDDTLDLGYPNISYSGKFTGDDQSIITCEHTAP
                     TVYAGVSGIFFNWGGYSDLINIHTGDTYVNILSGAYERWGDYTGSQRKYDEPGVVWMS
                     GTFGKYIDVFPFVQRNNATWIASLRSTVEPAVNVPVNSNMSPARLFPNPTADVFTTEI
                     NLSIAGIIECYLYDMQGRLVKSLLRAEGKAGNNHFQFSMAPLSTGTYQLYITLNSTAL
                     TTLPIIKN"
     gene            23836..25242
                     /gene="rlmD"
                     /locus_tag="PLL28_02500"
     CDS             23836..25242
                     /gene="rlmD"
                     /locus_tag="PLL28_02500"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNF68212.1"
                     /translation="MARNKKKIHQHVLIESVANSGSAFGRVEGKVVFTEGVIPGDIVD
                     IQVARKRNGYDTGYVTAIHKKSDLRIEPFCTHFDICGGCTWQNVEYETQLQFKQQLVD
                     DAFRLLKRSPGFPEIEKIIASPYDRYYRNKMEYTFTNGAYIFDPHYDKSNPPEKKPGC
                     GFHVKGQFANVFDMERCYLQGSLSNEIRMAVRSYALMHGFAFYDLRNHTGYMRNLIIR
                     NTGTGDLMVILVVALDEPEKLLPMMEHIRSTFPEITSLYYCINTKHNDVIYDIDVTLF
                     AGKPHMIEEIDGIKYQLGPRAFFQTNTEQAKNLYRKTIAYAALKPDDKVYDLYTGLGS
                     IALQAANKCSKVVGVELVEAAIADAKENAKLNNIDNCTFIAGDMAKIFTDDFIAEHGK
                     ANVVITDPPRAGMHKDVCLQLLKLAPERIVYVSCNPVTQARDIQLLIDDYKVTALQPF
                     DQFPQTYHVESIALLERR"
     gene            25500..25985
                     /locus_tag="PLL28_02505"
     CDS             25500..25985
                     /locus_tag="PLL28_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HNF68213.1"
                     /translation="MSENYEIDKVDIQILHLLMQDAMTPFTEIAQKIGVSPGTIHVRM
                     KKMDAMGLIKSSHLMVNPQKLGYGITAFLGVYLDKSSMYDSVVKELEKIPEIVDCHYM
                     TGTYSMFLKIICKDTNHLKKVLHDGIQPINGIDRTETSISLEESFSRPMKIELNGRKD
                     F"
     gene            complement(26024..27238)
                     /locus_tag="PLL28_02510"
     CDS             complement(26024..27238)
                     /locus_tag="PLL28_02510"
                     /inference="COORDINATES: protein motif:HMM:NF022779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HNF68214.1"
                     /translation="MALTDETLLKLETALSPLDSKKWQHTMSRVLRMILEFFFVALIV
                     SMFIGFLSMMSNGEMGDDMFFRVIRGFMLFVLAMVLFGIIRKTARDRFLSMQSAIAHQ
                     PWTLNLITIVFTVCAFAIGYFAGTAFLGGTFSPTFMIKWAGAIGSIFFLLGPYMLLKR
                     IEDNFTQQYKSILFANFLPGIAAQARYVMNEHIGTQTFTESKLFPYQEISSLKGSDLI
                     TRSQEQFQCSFLDVMQVERTQSNGKSETKYSTLFKGYLLSAAFNKSFTGETFVAPDAS
                     RELLGEVYGESLNELFHRPGTSIARMEDPDFEKAFSVYTTDQQEARYILSTKLIHRIM
                     ELKTYFVDDVYLSFVGNRVFVAVKSEKDIFSPPTIGNTYNKTLAEKQYAMLNALVTVP
                     ELFDLDRRIWAS"
     gene            complement(27253..>28116)
                     /locus_tag="PLL28_02515"
     CDS             complement(27253..>28116)
                     /locus_tag="PLL28_02515"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNF68215.1"
                     /translation="AATTGDNNTDNVEYVYLATPSAGDYKISINHKGTLSGGDQDYTI
                     VVSGLSCIPYSFTENVTITAPATYTLPNGSVVSTSGTYTSNFNTSLGCDSTIITNLTV
                     NTFTCAAPTGLFTNSITSNSARVNWTAVPGATKYQIYYRPVGGATWIKKTATTNSKKL
                     TGLAANTTYEYKVKTQCDPYPASGFSALVNFTTLPLREGEITSESESSLVIYPNPAEE
                     NCTVSAEGIADGVYQMYIINLQGQKVVEQTILFSGDDYTVDVKNLASGMYNLVLSNNN
                     THISDILVIAK"
BASE COUNT         8334 a         5542 c         5913 g         8227 t
ORIGIN      
        1 ggatcgggta tcgacagagc acaaggcgat tatatgggca tgctggcaac tgtcatcaac
       61 gggatggctt tgcaaagtgc acttgaaaat gtgggttgct atacgcgatt gctgtctgct
      121 atcaaaatgg agcagatttg tgaaccctat atccgtcgac gtgccatgag gcatcttgaa
      181 aaaggaagga tagtaatttt tggagccgga acaggaaacc catattttac taccgacacc
      241 gcagcaagtt tgcgcgctat cgaagtagaa gcagatgtga ttctgaaagg aacaagggtt
      301 gatggtgtgt atacggcgga tccggaaaaa gatccaacag ctacaaaata tgattcaata
      361 agtttccgag aggccattgc taaaaatttg aatgtgatgg atatgacagc atttacttta
      421 tgtgccgaaa ataatcttcc gatcatcgta ttcgatatga acaaggaagg gaatctggca
      481 aaactcatca gcggtgaatc tgttggaacg cttgtaaaaa attaatcagg taaagaaaat
      541 tatacagcta tgcatgaaga agaattaagt atggtgattg atatggccac tgaacaaatg
      601 cagaaagcca ttaagcacct tgaattggaa ttgtcgcatg tgcgcgccgg tcgtgcaagt
      661 atcgtaattc tggatggtgt gcgcatcgat tattatggcg ccttaacgcc attaaatcaa
      721 gttgccaact tgtcgactcc cgatgcgcga acaattgcca ttcaaccatg ggataaaaag
      781 atgctgagtg aaattgaaaa agcaattctt gcggccaacc tcggacttac acctatgaac
      841 aacggcgata tcatcagact taatttcccg ccacttacgg aggagcgtcg ccgcgacctg
      901 gtgaagcagg taaaacatat gggagaaacc acaaagattt ctattcgcaa tttcagaaaa
      961 gatggcaatg aggaaatcaa gaaattgcaa aaagacggac tcgccgaaga catggcaaaa
     1021 gatggcgaag accgaatcca aaaactgaca gataaattta ttgccgaagt agataagcac
     1081 cttggggtga aggaagcgga gattatgaca gtgtgagggc gtgaatcgtg agtcgtgaat
     1141 cgtgagtcgt gaatcgtgag tcgtgaatcg tgagtgtgag tcggagggat gtggttcgtg
     1201 tgcggggtgg tatttgttag tgtggtgtgt tatgttgctc gcttgtgtga tgtttggttt
     1261 tgtgattgac attaatgatt tttttgaatg catgattgca ttgcaatata tattttttgt
     1321 ttaaccctta ataattagtt cttatgtttc ttgatttacc gcataagcgt atggatgtat
     1381 atatccatgc gcgaaagttg ttattggagt gtcgcaaggt tactgatagg tttccgcacg
     1441 aagagaagtt tgttcttgca ttgcagatac gaagagcggc cctttctgta tttctcaaca
     1501 tcgcagaagg gtcctctagg ttttcgaagg ctgaacgcag gagattttat gagatatcca
     1561 gaagctcaat tgttgagcta gatgcagcat tcgatattgc cgagacctat ggctacactc
     1621 aagcagggct gctaagcgaa ataggagaat tgatagtcag attgttccaa atgctaagca
     1681 aattgataaa caatagtcaa tagcaaccaa attttttaat gtgatgattg gcattggctc
     1741 caacattcag cgagaatttt tttattaatt tgaatttgcg taaaaattta ctggttgaag
     1801 gataaatatg aaattacata gcgtgtattt aggttaaaaa tccaacaagc cacgcttaaa
     1861 agtcgtaatt aacagtccca agccactatt tagtcacgaa tcatagttac aacccacgag
     1921 ctacactcac gactcacgag tcacgattca cgatcgaata tttaaatgat aaacaataaa
     1981 tctctcaata aactatgagt aatatacgta tcgaaaaaga caccatgggc accgtagaag
     2041 ttccggctca tgtgtattgg ggcgcgcaga cacagcgctc aatcatgaat tttcctatcg
     2101 cacaggatat taataaaatg cctaaggaga tcattgccgc atttgcttac ctgaaaaaag
     2161 cggctgccat gactaaccat caacttggcg ttctcgataa atcgaaggcg gatattatcg
     2221 caacagtttg tgatgagatt cttgaaggaa aattaaatga tcaattccca ttagtggtat
     2281 ggcaaactgg tagtggcacg caaagcaata tgaatgtgaa cgaagttgtt gcttaccgcg
     2341 cacatgtgtt acttggagga agcctgatgg atgaaaaaaa ggctgttcat ccaaatgatg
     2401 atgtaaataa atcgcagtcg tcgaacgaca ctttccccac agccatgcac attgctgcgt
     2461 ataaaatttt gttggaatgt acaattcccg gtatagaaaa actgcgcaat acactggctg
     2521 agaaatcgtt ggcctttatg catattgtga agatcggaag aacacatttt atggatgcta
     2581 caccgcttac cctcggacaa gaaatgagcg gttacgtttc gcagttagat catggattgc
     2641 gtgcaattaa aaattcactt gcgcacttaa gtgagttggc attaggtggt actgctgtag
     2701 gaacaggaat caatacaccg gaaggatatg atgtattggt agcaaaaaat attgcagcgc
     2761 ttacgggatt gccttttgtt acggctgaaa ataaatttga agcactcgcg gcacacgatg
     2821 ctattgttga atcgcatggc gccttgaaaa cagtagcagt atcattgatg aaaatcgcga
     2881 atgatatcag aatgttatcc agtggtccgc gctcaggaat tggtgaaatt catattcctg
     2941 acaatgagcc gggttcatct atcatgccgg gtaaggtaaa tccaacgcaa tgtgaagcac
     3001 tgactatggt tgcggcgcaa gtaatgggca acgatgtggc tattaatatt ggcggcgcga
     3061 ccggacattt tgaattgaat gtatttaaac cggtaatgat ttataatttc ctccacagcg
     3121 cgcgtttgat tggtgatgcc tgtgtatcgt tcaatgataa atgtgcgatg ggtattgagc
     3181 caatagaagc gaacattaaa aagcacgtag ataattcact gatgttggtg acggcattga
     3241 atactaaaat cggttattat aaggctgctg aaattgcgca aaaagcacat aaagaacata
     3301 aaacactgaa ggaaaccgct gttgagcttg gatatttaac acctgagcaa tttgatgaat
     3361 gggtggtgcc ggtgcaaatg gtgggcaaat tataaatagc tgattcacga aaaaaaaata
     3421 aaacctgcat tcctaaaaca tgcaggtttt attttttgtg ccgatttcaa tttttgtata
     3481 agttgaaatc gacttacaac tgcggtattt tcctttacac cgtcgattgc tatttgggaa
     3541 atacctttca gttgttgtat atgtttacaa aggaatagtt acttcaccca gatctgtgat
     3601 aatacccgtt gtaacactga caccgggtaa agtaatatct gtaaatccgg ctactgctga
     3661 aaaattaaca gaataacttc cttccggcaa acccccaatt aaaaatgaac catctgccga
     3721 gatataagtg ccggcggtat ctgttgccga cattgcctga acataatatg cggcatccgt
     3781 aggaaaaaca actccggtaa tagcacctga tagcgcctca gtataaactc ttattacagg
     3841 ttttaatgaa tagtcgccat ttcctttttc tacaattgac tgctcagcat caaagtccaa
     3901 ccaaagatca tagacaagac ccgcttcgaa tgtatagtga acattcagtt tcaatccgga
     3961 tgtttgtgca gaaggtgttt ccaaaggatg agattctcca tcaacaacta ccgtattgtt
     4021 agaacctaat atcaatcgaa tttgtgagat tgttctaggg gatacatctt cagaaatgat
     4081 caatgtgtcg atgcctgaat taaattccag taaattatat acccccggag ttaccacatc
     4141 aaatgtttct actcccatat ctgaggtgat ctctacagct tgaatgtcga tgtatactgc
     4201 atcatattcg gcaggtgcat cggttaaata aatgtttaca tgtgcctgtc tgtcagctgt
     4261 gtcatcttta caactgctcg ttgctaccaa aactaccagg attggcaaca tcacaatcat
     4321 acttatgata ttctttctca tagtgattca gtttgatgta atccatacaa atcagatgcc
     4381 atcgctataa tttattgaaa atcaatgagt ttattgcgta tggaaattga tttattgagg
     4441 aattatttcc ccattgcatg ttgttttgac atcaattttg catgtatcaa tttttggaga
     4501 tctacaatga tgtaattaac ttttattctt tcagaaattg tgaaagtcat ttctttacac
     4561 aagaaaatta ctgaccaatt gaaaacatta catatttcac tcattggatg atgtaaagcc
     4621 tggcaaaata ttattgcaag catgagatca catccgtcta ccggcacttt cggcgatatt
     4681 gcctgtttac cgtgaatgtg tactgtttag tatgcacagg gtgatttttg tcggaattgg
     4741 attgtgtaaa aagatcattt ggaaatcaat cctaatctat taccttagca tcaaaactca
     4801 cgcaaaacaa gaaaacaaac tactcgttgc ggcagtcgcc cttacaatgg tgttttccgc
     4861 aaatcgtgct tctgcacaag ctttttatca aggcgttaat catctgagcc ttggtgttgg
     4921 atttggtggt tatctcgact atgcctacac cggaagcggt gatttcagcc atatcccaac
     4981 gctgtttgca tctttcgacc atggaacaat cgatgatgtc ttcggaggca atatcggaat
     5041 tggcggtttt gtgggatact cttctgcaaa atacaattat gattaccttg gttatcatga
     5101 tgattctaag tggacgtatt tcgcaatcgg tgctcgcggt acttaccatt acatcttaga
     5161 taacgaaaac cttgatcttt atgggggtat cagcattggt gtgttgtcgc agagctacga
     5221 ctacaattca aattatccat gggtaggatt tgatccaaac tcatactacg attataataa
     5281 tatggtattg tattcatccg catgtgtagg tgcaaaatat atgttctctg aaagtctcgg
     5341 cgcctttgct gaacttggtt gggacattgc ctggttgaaa gcaggtgtta cacttggact
     5401 ttaatacatt ccaacaataa aaaaaccggt tgtgcaaaca gccggttttt ttattttata
     5461 ttagttgcaa ttattttatt tcttcataat caacataatc cgcatccttc gaatttgtct
     5521 tttccgaagt cccggcagga ttctttttct cccgtatgtt attcccccct tgctgttgtg
     5581 acgattgacg ggctccatgc aacattggct tgatgaaggc gatgtaaatg atgatgataa
     5641 ctgctatcca ggcgacgtcc gacatgatga aataggagag aggaaaaagg agaaaggaga
     5701 aaggttgatt aaagtgagat taaaatttga gcaaaattat gactttattt ctgagctaag
     5761 gtaatttata aggttagaaa gttgttgaaa gcaagtgttg acagcattat ttaaggtgtg
     5821 gagttctgtg ttttgtatga ggttcaaggt taccaagata tcgaaaccag agtccacctc
     5881 aaccaaagat cctcgagcca tttcataata tcgtcttctg tcaattttcg aacgtttaga
     5941 tgcaccttca gcaatattta aatggacgga aacagcagca cgcttgatct gcttttcgat
     6001 accaaaccgt tctgaagaat ttacagtatc taacacctta tagcattccc ttacaaaggt
     6061 tagactggcc aaatatatgg gcagtttttt gtgcgagaga tccaaaaaca tacatggtat
     6121 tttaaaggta aacaatcccc taaaattaac cagtaataac aaatcaagtt taatcgctta
     6181 tcatccattt aaggctaagt aacagccaaa tagaacccta tttgcgcttt ccacctcacc
     6241 ctcctctctc ctctctcctc tctcctctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnnnnnct cctctctcct ctctcctctc tcctctttcc tctttcctct ttcctctttc
     6421 ctctctcctc accttgaaag aaacatcgac ttcttcgaat acatttccct aaagaacgga
     6481 tcctgcaaat cggcgatgaa ccggatggct tcgccggtgg atttcatttc agggccgagc
     6541 tctttgttga cgttagggaa tttttcgaaa ctgaaaacag gttccttgat ggcatagccc
     6601 tgtaattttt tgtcgaaggt gaattcgctc agttttttaa ctcctagcat caccttggta
     6661 gccacattca aatatggaat ctgataggct ttggctatga atggtgttgt tcttgatgca
     6721 cgtggattcg cttcgataac ataaacttgc tcatttttaa ttgcgaactg aatatttatc
     6781 aacccgcgta tattgagtgc gaatgcaatc ttttccgtgt atgctttcat ttgttccagc
     6841 acacgttcac ttaaattgaa acttggtaat accgcactcg agtcgccact gtgaatacct
     6901 gccggttcga tatgctccat gatgcccatg atgtgcacgt tttcgccatc gcaaatcgca
     6961 tcgatttctg cttcttcagc gcgatcaagg aagtggtcga tcaatattct gttatccggg
     7021 aaatgtttca aaataccgat aaccgaacgc tcaagttcct catcgttgat tacaatccgc
     7081 atgcgctgtc cgcctaacac ataactcgga cgcaccaaca ccggatatcc gatgcgatgt
     7141 gccacttcca atgcttcttc aacagttgtt gctgcaccat attcagggta tggaatgcct
     7201 aaatctttca gcagatcaga gaacctgccg cgatcttccg caatatccat atcgttatag
     7261 gatgttccga taattttgat gcctttttca tgcaattgct tggcaagttt taatgctgtt
     7321 tgtccgccta actgcacaat aacaccttca ggtttttcat attcaatcaa ttcccataag
     7381 tgctcccaga ataccggttc gaaatataat ttatcggcaa tatcaaaatc agtagaaaca
     7441 gtttcaggat tacaattcac catgatagcc tcatagccgc tctcgcgaat ggccaataca
     7501 ccgtgcacac agcaataatc gaattcaatt ccctggccta tcctgtttgg acctgaacca
     7561 agtacgatta ctttttttcg atcacttaca atactttcat tttcatcttc gaaagtagaa
     7621 taataatatg gcgtttttgc ctcgaattca gctgcgcagg tatcaaccag tttataggtg
     7681 cgtttgatac ccaatgatct gcggtgattg taaacttcct cttcggtgca attgccaaca
     7741 gtatttgcaa tttgaatatc gctgtatcct ttttgtttta attcaaggaa aagtttttta
     7801 ggaatattag tgagttggta tctgcgcaat tctttttcaa gttttaccag atcttgtatc
     7861 tgcatcagga accaaggatc tatcaaactt tggttgtgaa tggtgcgcac aggcacgcca
     7921 aggtccatcg cttttttaat gttgaatatt ctgtcccagt gcggttttgc cagactgtcg
     7981 atgatctctt gtgaagattt gtaaaatccc ttttcaccac tcaatccgat tttattgttt
     8041 tcgagacttt gacaggcttt ctgcagtgct tcctgaaaac tgcggcctat actcatcact
     8101 tcaccaacac ttttcatctg caatcctagg gtgtcatctg caccgggaaa tttatcaaaa
     8161 ttccatctcg gcatttttac taccacataa tccaatgcag gttcgaaata tgctgatgta
     8221 gattttgtga tttgattatt gagttcatcg agattataac ctatcgccaa cttcgcagca
     8281 atttttgcaa tcggataacc ggtagccttt gatgctaaag ccgatgaacg cgatacacgt
     8341 ggatttattt ctatcgctat caggttttcc gtatatggat cttgagcgaa ctgcacatta
     8401 catcctcccg caaaatttcc catcgcacgc atcatcttta tggcgaggtt gcgcatatgt
     8461 tgatagcaag tatcactcaa tgtcattgcc ggtgctaccg tgatcgaatc gccggtatgt
     8521 actcccatgg gatccaggtt ttctacaacg catataatta ctacattatc attagcatcg
     8581 cgcagcaatt caagttcata ttctttccag cccaataccg cttgttctac taatacctca
     8641 tgcgttggcg aggctgttaa tccgcgcatc aaagcagcat caagttcttc ttttaccttc
     8701 acaaagccgc cacctgttcc gccaagcgta aatgatggtc tgattacgag cgggaaacca
     8761 atttcctggg caaattcttt tccctcaaga aatgaattcg caacatgact ttttgcaaca
     8821 cctatgccca attccaccat caactggcga aataattccc tgttctcagc gcggtcgatg
     8881 gcttcgatat ctacacctat catccgcaca gaatatttca cccatattcc caactcaaaa
     8941 gcttcctttg caaggttgag cgccgtttgt ccgcccatgg ttggtaacac cgcatctatc
     9001 ggtgtgcctt tttcgatatt ttctatcaat attttctcga tagatcccac attcaacggc
     9061 cacaaataga tttgatcggc agtaaccggg tcggtcatga tggtggcggg attggagttt
     9121 attaatgaaa cttttatccc ttcttcgcgc aaactgcgac tcgcttgtga tcctgaataa
     9181 tcaaattcac aagcctgacc aatgatgatg ggtcctgatc caatgatcaa tacatgctta
     9241 atagaggtat ccttcggcat aatgtccgtt gtatgataat aaggttagga aaataaaatg
     9301 gccttgggga gtaaaagaga gctgatgacc ggcctgaaaa tgcatttcag gatgcaaatt
     9361 tacggtactt cgaccgcttt tcctgctact ttttcagtta tcaacaatcc atgcatgaaa
     9421 atgccggtta ttcaccgctg gtagagcaaa aatggtggat taaagcttcg gctcgtcgta
     9481 ttttcgcctt atgacagcag ctggggactt tcaatttggc gatgcagtgt ttcattatac
     9541 gaggtttggc aatggaactg aggtcatgtt ggcttttcat ggcttcagcg agcccggaac
     9601 gagctttttg cccctggagc ctgccctagg cctccgctat acgatttatg ccttcgatct
     9661 tccctggcac ggagaaacgc aatggcctga agggcggatt ttcacgcctg cagacctcct
     9721 gggactcgta aggggctttc tggactactt aggcgcctca aaatttgccg ttttagggtt
     9781 cagtatgggc gggaaatgcg ctttgtacat caccaaacat atgcctgaca ggctcaatgc
     9841 cctgtggttg ctggccagcg atgggattca aacgaacaga atctacaatg tggcggttta
     9901 tccggcatgg ggacgaaaac tgtttaaaac aacgattagg catccatcat ggttctttgg
     9961 aatggtgaat ttggcaacga ggctgagatt gatcaccccc tggctgaaga aatttaccaa
    10021 taaccatatg gctacgcgtg aaaaacgtca acggctctat gatacctgga tcagcatggc
    10081 aggatttaac ccggatatcg aactggtgaa aaaacaactc aatacctttc atatacctgc
    10141 attattattt ttcggacgaa gagatgaagt aataccgtac acggtaggcg aacaatttgc
    10201 cgaaggggtg gcgcttgcaa aattgaccgt gttagagcgc ggacattatt ttattgatga
    10261 gaagatcaat cctatgttgg aagaagctat gcatttcatt tgaagattac gtaggtctga
    10321 tgatataaac tacatttgta ctgcatgcta tttatcacca ttggcaccat tttacttatt
    10381 gcaggctacg tttcactcgt ggcatttttt ctttatggat ggtggaagtt aaaggaattt
    10441 aacggcactg ctacaaaaaa gcatgtattt attacggtaa ttgtgcctgt caggaatgag
    10501 gaaaaaaata tcgtacactt acttcgcgat ctgaaggagc aaacctatcc tgcagacaaa
    10561 tatgaagtga tcatagtaga cgatcattct acagatcaga caacacagaa agtgatgcag
    10621 gcgaaaatgg ataatgtgcg cattatttca ttgggagatg cgggaactgt gcgcatgaac
    10681 aaaaaagcag gcattcaaca tgctgtttca tcggcaaaag gtgaattgat tattacaacg
    10741 gatgctgatt gtcacgttgg caataaatgg atctctacta ttgcggcata ttataccgaa
    10801 catgaacctg taatgatcgc cggtttggta aactattttc ctgatgcaac attttttgga
    10861 aaatttcaaa cccttgattt tttaagtctc gtgggtattg gagctgcctg cattcaaaac
    10921 gggttttaca atttatgtaa tggtgcgaac ttagcatata caaaggcagc attcgaggca
    10981 gtgaatggct acgaaggtgt tgatcatatt cccagcggcg atgatatgat gcttatgcat
    11041 aaaatggcgc ggaaatttcc gggaaagatt gcctcgctga aaaataaaga cagcattgtt
    11101 tacacacata ccgaaacaga tgtagtctca ttttggcatc aacgtgttag atggacgagc
    11161 aaatcaacac attatgaaga taagcgtatt acattgatat tgagtttcgc ctatctgttt
    11221 aatttactga ttgtattcaa catggtaatc gggttttttg tgcccggcat gctaaagctt
    11281 tcaatgtgga tgtttctggt gaaaatttgt attgatacat tatttacgta tgccgtaagc
    11341 agattcttca ggcgcgaaaa tattctttgg ctttttctgc ctatccaggc attacatgtg
    11401 ttgtatatta taataattgc accggcaggt gtgtttggta aatatcaatg gaaaggtcgt
    11461 cgccattaat tcaatgaaat ttaaacggaa attacttagc atactgatat tcgttgccgc
    11521 atccggggca attgcagttc tgtggaaatt actcgacacc gatatagatt ggttcgtggc
    11581 gatttacaat agtataaccg cagttatggt tatttatgtt ggcacattaa tgttatttat
    11641 ttacccgatt gcctttctta ggaaatactg ggagaaaaag aaatcgcaga tagacctgaa
    11701 tgcgaaaccg aaatataaag tgtacgacag gttgatgcgc aatacaaatg cacttgcagg
    11761 aggtttgttt attctatggg tagccttagc tgcaatattt gcacatatca ttatcccgga
    11821 taaatcgcca tacgcaggag atcagatgaa tgagattgcg tataaagagc caggattttc
    11881 tgtgaaggtg ttattagttc cagccgacag acacgtttca tcgacaggcg tgatcgattg
    11941 gtttttgaat ggcaagactt catcgtttaa acaaatacca atcaatgatg catggttggt
    12001 gaagggcgac tcactttatg tttcgcggta tcttggagat aatatggttg gcattcccat
    12061 ggtgtttgcc actgaattta tcacaggaaa aaaatctgtg gtagccgagc aaagccaaat
    12121 aattgagaat gaatttatcg aaacaaggca tttctggttg gggaccgatg atttgggcag
    12181 agattattta agtagaatta ttcttggtgc cagggtgtca ttatcagtgg gcattattgc
    12241 ggtactgatc gcattattta tcggtatacc acttggtgcg ctggccggat tttataaaca
    12301 gtatcctccg aaattacgtt ttgggaaaaa gcgtgcagta aaagttcctg tagacaatag
    12361 tgtgatgtgg atgataaata ttgtttggtc tattcccaca ttactgttag tgtttccaat
    12421 tgtttttgca ttcggtcaac agtattatac tatttttatt gccgttggta ttaccatgtg
    12481 ggtagatatt gcgcgcatgg ttcgcgggca ggtaatgcaa gtgcgtgaaa tggagtttgt
    12541 gcaggcagct cgtacctttg gttttagtga taccagaact atttggcgac atgtattgcc
    12601 aaatatcacg ggtccggtta tagttgtaac ggctgccaac tttgcttatg ccattcttac
    12661 cgaggcggga ttgagttttt tgggtattgg tgtgcaacca cccgcaccga gttggggatt
    12721 gatgattgcc aaatacaagg ataacctgat tacagatcca tatttaccct tgatacccgg
    12781 atttgccatc accctgctag tgcttgcatt ttttatggtg ggcaacggat tgcgtgatgc
    12841 actggatata aaaagcaggc tgtaagcagt tgttggaagc aaaatatttc aattgtgcag
    12901 acactttctc tttcggtaac aattatgtga ccattcattt acacttcaat acacctcata
    12961 tcttactttt atttgcttat tttgcatggt cgattgatca atcatggcga tagcaggact
    13021 taatgtaatg gaaatgttgc gcaaccagtt gaagttaaaa caactggaga tagattcgct
    13081 attggagatc acgaagtcga tcaatgcaaa taaatcggca tctgctctgt ttgaacttta
    13141 tcaccgcacg ttaagagatc agattggcgt tgaaaattta ttgctcttca gtaatatggg
    13201 tggcgattgg gaagcgatga taggcgataa gctgatttct gaagaccacc cggaagtgat
    13261 gcagtatttg ctgccctatg aaaaacttac tgatttaact accgtagata ttcctgacta
    13321 cctcagtgaa tatgacttgt tgattccggt gttgcataaa gacaaaccgc tggcttattt
    13381 actcatgagc aatccacggg ttgaaagtta tgagccgctt gaagacaaaa ttaaattcgc
    13441 acaaacggta accaatattg ttatcgtagc cattgagaac aagcgattgt tcaagcgcga
    13501 agtagaacag caggcattca agcgcgaact cgaactagcc gcacaggtgc agagtatgct
    13561 cattcccgat acccttccgc ataccgaaca agtgcagatg gcagccattt atctccctca
    13621 caaaaacgtt ggaggcgatt attatgattg ccttgagtta aacaataagg aaatattttt
    13681 ctgtattgcc gatatttcag ggaaaggaat tgcagctgct atattgatgg caaattttca
    13741 ggcacaaatt cgggagctca ctaaacgcaa tccgccaacc atcgaagagt atatgcagga
    13801 attgaacaaa ggtgtgttga tgagcactaa aggtgagaaa ttcatcacgc tgttcctggc
    13861 aaaatatgat cgcactacgg gttatctcaa atatgtgaac tccgggcata atccgccaat
    13921 attagttaat agtcaaggga cgaaattgtt ggataaaggc acaacaatac tcggtatgtt
    13981 cgacactctt ccttcagtga aaatggggca attgccgatt acggaagaca cagcaattgt
    14041 ttgctatacc gatggattga ctgatgtggt aaatgagttc aatcaggtgt tcccacttga
    14101 tgaattaatt gagttggcaa aacaacatta taaagaacct gtagaggtgt taaataaaag
    14161 catcatcgac gagatcatgc atttcaaagg ccatgacggt gaagtgagtg atgatatcac
    14221 tgtgctctcg gtaaaaattc atcccgcaaa ttaatcttta gccggagctt cagcgcttaa
    14281 ggcaggattt cttctaagta ataaatcgaa acttaccagt agcgatagga acatgaaaaa
    14341 tgatgtacct attttatctg cttccaccag atcgctcaat gccaggttta taatgatggt
    14401 aatgatcgac aaggttatcg acatggaaat atgtttatac tctatgttgg taaatcgatg
    14461 atacaatcgc tggcctaaat acatcgtata gattaatagt actacccaaa caaaagtgcc
    14521 gggaaaaccc tgttcggtgg ttacctgcaa ataataattg tgcacagttg atcgctccgg
    14581 gtttttactg atatacgttt cgaatttatt tacggtatat tctttatagt tgaaataaaa
    14641 ctggccggga ccaaatccca tataaggctt gtcttctatc atgtgaatgg cggctaccca
    14701 acgatagatg cgttcggcac tggaaacatc ctccaacgac atggtagatt ccatgtgctc
    14761 attgaaattg gtatgataaa tcgttttttt gtaatcagga gcataatcca aatagcgatt
    14821 attatgcaac atgactattg caaaaatgat aacgccgaac accgcaaata cagcacttgt
    14881 tactaattta ttcattctta tgatgaaaaa tgcaattgta gcacctgcaa cgcttaacca
    14941 ggatgatcga gtataagaga aataaatccc ggcgataagc acaactatgc caaattgaat
    15001 gatccatcgc tgccaggtat ttttgggata ccatctgtat gctgaaaaga gcattggaag
    15061 catcaaagca agaaatactg cataattcac gtggttccgg aagaaaggaa ccattgtttt
    15121 gtttacttcg ctgaaagcaa aatgatactt attgtgattg atgagggtgt aaatggtcag
    15181 cgcaagtgtc gggacaaaca gacaccagaa agctttttta aaggtggcga tatcttttat
    15241 caccagcgag gtcatgaaat aaaacgtagt aatataccat gtcttcgcca gcatatattt
    15301 caaagaaata acaatatcct ctgaaaacat ggtggtaaca agcatccagg caaagtgcac
    15361 gtataatatt atcgtcaatg gatgcaaaaa gaatcccgta tcaaacaatt cacgtttcaa
    15421 aaaaatgaaa aaaataaacg tgaacataaa aatgatcatc atgggctcag taggtaatgt
    15481 ggtactgaat cccgacgatg aataatattc aatggaaaag ggtgtagcaa tcaggagcag
    15541 aaaaaaaatc actctgaagt ctacaatcat ttggtaggca aacagaaacg caacaggaac
    15601 tgcaaaatac aatggttcct ctaaaatcac accggcgaaa atcgcaacaa tggcgagcaa
    15661 tgttgcaatc cagtatatag aaaactcaac tggtttttcc agcgttttaa gcattttgaa
    15721 tgcgagattt gatctttctg tagttttctg taattactgc gagcaaaatc atgaaaacga
    15781 tagtgccaag tgttacaccg atcacgatca acgaacgaac gggtttgtcc ttgcgtaaag
    15841 cagggtaggc ggcctctgta atatgcacag tgctgaaatc ggtattcaat gtagtttcgt
    15901 tctgctccaa taatttgcga tcaagattaa ggtcgtcaac ggcgctttgt aatttgctgt
    15961 cgagaacgat catcatttcc gctgatgcac ctgctttgcg ctgcgcagcg atgctatcgg
    16021 taagattagt aacaattaat tcttttttgg ctacctgatt tttaaatgta tttacaataa
    16081 gctttttatc gcgcataata atgtctttat tatgctgatc tattgtttca acaatatggt
    16141 tcgcaatatg cattgcggta tccttattct tatcccaaac tgaaacttct atcgcaccat
    16201 attctgtttc tactgcacta tagttcttta aaaattcttt cttgcaattg tagcgcgcca
    16261 tttctttggt agaatcgata tcataatgct ccatcaaatg gaagtgattg atcacataat
    16321 cgattaagct gctggtattg gcgatggtaa gaatgcgatc tacgtcactg gcagtaccga
    16381 aataatcaat ttcgatttcg ccggtactct gactaaacaa tacctgcctg tcggtcatga
    16441 tgggattcga aggataaaag tttaccgtcg acttaaaata gtcgggcata aagaaccacg
    16501 taatgataca ggctgcgatt gccacaacca ccgttgtaat gatgatgggt ttacgccatt
    16561 taaaaatgat ctgcaataac ctgatcaggt cgttcctttg ttccatacat gaagtttagg
    16621 gtgcaaaggt aggaaaatct gcctaagagc cttgcctgcg tgctattgcc gactgcatca
    16681 ctgttttgag gctccggacc tcagtcatat tgatgatacc tgcgggtatg gcgaggaagg
    16741 cgccggatgc gagcccaatg acccattgcc atgtccagga aatattaaaa acggcaataa
    16801 gcaggttcac accaattacc gctgctagat aaaatatcga ccgcagccat ttggagagat
    16861 ttgagcccag cgaaaacaag cgatgagcca cggcgatatg tgctaataat atcaatacct
    16921 gagcaaaaac tgtagccttt gctgaaccta gcgcttgttg ttcgggaatc agcatcaggt
    16981 ttaatatgat attaagtatg aaccccccaa aactgatata attcagcatt ttgatactgc
    17041 catgtgcggt gatgagactg ccatatacat aagtaaggct tatgcaaatg aaacttgcca
    17101 tcaaccagcc aaaaatgctg cctgcagagg ggtcatgcac atgataaagc aactgcatga
    17161 tgtcttcacg atgtatcaga caagcgatgc ctgctgaaat actgcaaatc aacatgattt
    17221 gaaacccgag gtccagtaaa ggttttacgg atgtgttttg ggatatcatg cgggcaaaca
    17281 ttggcatcaa catccccgcc attaaaaagc cgagtatgct catggcatcc aacaatcgaa
    17341 atgcggaggc gtagatgcct gcctgataag gacccttatc gcccaacatc cgctccagca
    17401 tcactccgtc ggtacggtaa taggctgtca tcagaaaccc gagtaaggca taaggcaagc
    17461 ttcccttgaa aatgtttttg atcagtgctt tgtcgatgcg gtttgaaatg gtaagctttt
    17521 gtctcgaaag cactgcaacg gcaaccagta tggccacaac aaagcctcct atctgtatcc
    17581 aaatgaataa tggaattgac atggttatgc ctgatgcacc ccaaaatatc caggcacata
    17641 aaatggatgt ggtgagtttg tccagtatag acaatacagc atctgtgcgg aacaaatgca
    17701 aagcattgac attgctgcgc aagaaggtgt aaaatgacaa ggcgatctgc atcaacacca
    17761 gtagtccgag aatttgaaat tgttcgcctg aaaattgcac gccaaaagca gcgataaata
    17821 ctattatggt atataccact cctaacaata gcttcagcac caatgtcgac atgatgttac
    17881 gacccaatgt ttgaggatct tgagctacct ggcgattgtt gtaattgttg atgccgaaat
    17941 cgagcaccac ctgaaaaaga taactgaaat tgaacagagc aaaataaaca ccataaacct
    18001 ccggacccag cgtattctgc acggtcctgt cgatgcccaa tatccaaaat ggcttcacca
    18061 gcaggttcag aaagagtaaa aagccgatgt tggtgataaa atttttgcgc atttttttgt
    18121 tgaagtgtta aaaatagcta ttattgcttc aaattctacg ctagcggtca acatctactc
    18181 taaagtttcg tgtctcgact aacctctacg ctttttctgt ttattggtaa aaacccaaat
    18241 gtataacatg ctgatagctg ttaatacccg tttccttatt aaggataaac tggaaggcat
    18301 aggatggttt acatatgaga ccatgaaacg cctcacccgg aaccacccgg agcatcattt
    18361 tctgtttctg ttcgacagag actgggatcc tgaatttatt ttcagcgata acattacccc
    18421 tgtcaaggta tggcctccgg caagacatcc gtatttatgg aaatattggt ttgatactgc
    18481 cctgcctcgt atatttaaaa agtacaaacc cgatctgttt atttctaccg atggcatgat
    18541 gtctttagcg gtgaatgtgc caacagtatt ggtggtgcat gatcttgctt atattgaatt
    18601 tccacatcat atcccacgac tcacgtacga atacttaaaa cattttacgc ctaagtatct
    18661 ttcaaaagca gatgccatta taacggtatc cgattattcg aaaaaggata tcatagataa
    18721 atatcaaatc gcagatagta aaattgatgt tgtggctaat ggagcgagcg ataattttaa
    18781 accactggat gaagtcaatc gttcaattgt gaaacaaaag atcagtagtg gtagcgaata
    18841 ttttatttat gttggtagca tacaccctcg caagaatgta ggcatgctta tgaaagcgta
    18901 tgatcaactg agggcagaag gtatctgtga tcataaatta gtgatcgttg gtagatatgc
    18961 atggaaatca ggtgaaactt ataacatcta taagtctatg caatttattg atgatgttat
    19021 ctttacaggc tcaatgaatc aggaacaatt gacggaagca atcggtgcgg cagacgcatt
    19081 agtgtatcca tccttatttg aaggattcgg gatacctgtg ttggaagcaa gatatgcagg
    19141 tgtgcctgtt atttgttcaa acagtagcag tttaccggaa gtgggagggg aacatgccat
    19201 ttattttgat cccaattccg cagaggccat cacaacggct atggtccaat ttcttaaaaa
    19261 cagagccgaa ttgaaaagaa atgcactgga atcagcctat gaggcaagaa atatatataa
    19321 ctgggataat tcagcaaata agatttacag cataatgcaa gccacgtatg acaactatag
    19381 agcaaataga aaatatactg caaatacctg atacggataa gaagaccccg gttcgcgttg
    19441 aggaggccaa atttatttat cagtttattt taggtcataa gattcacaaa acgcttgaaa
    19501 tcgggttggg ttatggtcgt tctgcttcgt atatcatgct ggctacagga aagccacata
    19561 tcgctattga tccatttcaa tcccattatg gctaccaggc tattaaaaat atcacagatg
    19621 ccggcttaaa ggacaacttc acacatattg aagatttttc tcattttgcc cttccggaac
    19681 tatgtcacaa aaacgaaaaa tttgattttg tttttattga tggagatcat aaattcgatg
    19741 gtatccttat tgattactat tttgcatcaa tgttagtgaa aacaggaggc tatatcctct
    19801 ttcatgatac atggatgcga tcaacaaggc ttgtaaataa gtttgttgaa caaaatcgcc
    19861 atgattttaa aacacatccg gtatccctag ccaacctgca tctcattcag aagatacagg
    19921 ataatgatac aagggacggt atgtacttcc gcgaattcta tacaacaaaa tcgtggttga
    19981 aatatcggct aatcacccac cttaatgatc atcctaaagg tcttctgagt aaactcaaaa
    20041 gtatttttag accttaaatt tattactatg aaaaagcttt tttggttgac agcattatgt
    20101 atgctgttaa cacaggtttc tactgcccaa atttatgata tctattctgt gatccccaca
    20161 ttcaacaccg tagaaacaga caactattgc tcagacagtc ctattgctgt ggaagtagtt
    20221 atgacggcag gagccataac agatgatgat ggatttagta ttcgcatgaa atcatatccg
    20281 ggaggtctaa gttgggcagg ttatgatggt ctttgcccgg atgattataa ttgtgcttat
    20341 ggtttttcta tcgaccctgg tgatgtaccg gatggcttgt actattttga agcttcatgt
    20401 aaattttttg cccatgccgg cgacccggat tatgagcagt ctgaagtgtt cagtcttaaa
    20461 acaattacat gtggcaatgt tgaagaccca ttgaattgga cttctgcaaa agttcgtgat
    20521 aatacaggca tgtccatggc cgatggtaag atatattatg tggatgataa ccgtaaagta
    20581 cgtaattttt attggacagg tacaaattgg atctctgatg tgcttaatac tacagcccct
    20641 aaggccaagg atggtgttga catcgtggca acagtggaaa atgtgtatta tgtaggagat
    20701 gataataaga tcaaaaacct atattggaca ggcactgatt gggcttatac ttcgttagtt
    20761 gcatcgcaac ctgaggtgag aagtaattca aatattgcct tcggcgatgg aaagatctat
    20821 tttgtatcag ctgataataa caaagtatac aacttgtaca aggttggtgc aacatggtat
    20881 tgcagtgcat tgaacaatga cgctcctgat gttcgagatg atagtaagtt agcaactgat
    20941 gtaggtaaag tatacttcgt ttcttcaaca cctttgaatg catctaccaa taaaatgatc
    21001 tataattatt attgggatgg tgattcttgg gagtatgcca tgttgaatag ttcggcgcca
    21061 aaggcaatgg atggcgcagg acctgcagtt tgtaacgcaa aagtgtatta tactgcagag
    21121 gaccaaaaag taaaaaggtt gaaatggaat accgcaacag gcgtgtggga tgtgaattct
    21181 ctattggcta cccaaaagct agttgatgct ggtgagcaaa tcgcagttgt ggacgatatc
    21241 atcttcttcc ataactcatt tgatcttgtt tattacctga aagtaagcgg tcacgcatgg
    21301 aatgaatact caatgagccc atgcaacgcc tattcaagca cgtcgtattc ggatatcaga
    21361 gataatacaa ctattattcc tgagggaacc ggtaaagttt attatgttgg tacagacaga
    21421 aggttgcgaa atttctacac agtcgcctgc aggtcttcca ttgagatctc accggtaccg
    21481 accgaaatat taacggcatt tcctaaccca tcaaacggta ttattaagtt gatgaatgca
    21541 tccactgagc cattatcgat aaacacactc agggtcgttg acttgcaagg caagatggtg
    21601 tatgtagata cggaatctaa acttgtaaat gcaaatgcct ccttagattt ggatctgtct
    21661 cagataccca acggtgtata tttcatttta tctgatgcag gtgatgaaat tatatccaac
    21721 aggattatta tttcccatta atgaggtttt cttggtggta taccaaaaag tgatgtgatc
    21781 tttatttcat cgttcgattt tgatacaaca aacttaggtg gggagtccga ttatgggctc
    21841 cccacttttt tgtgccactg ctaaatttac tatctttgac tatggtaatt cgcatgcttt
    21901 tttctaagat agggctctat gtatcagcac tgggagcttg tcattgctcc atcgatatgg
    21961 cctattcgca aaaagtagat aatgatcaca gagatcaata cgctccttct gcagtggtga
    22021 tcatgcatga gataaagcaa gattataatc ctatctggta ttccactgaa atgccggctc
    22081 cgggttcgaa ctcttatcgt cgacacctga ttgatttgaa ggatgaatta taccgtaact
    22141 acatgcctca acaacaaagc aatttgttgc gtgaagaaac agacatcact tcaccggaaa
    22201 tattaagtgg ttgggaagga aatctgatca ccagctatgt tcccaatgat aacgatctgg
    22261 caatctcgaa cggcggcatg atcgtgtctg taatcaactc caatatttat atttatgatg
    22321 atgcaggaac aggattattc gttacctctc tgcagtcatt tgctgatacc cttggcatct
    22381 tagattctaa attcgaccct aaggtgatgt acgatccgcg tgcagataaa tttgtgttgt
    22441 tatttcttgc aggtttcgat cctgaacata cgcatgtcat ccttgctttt agtcaaacaa
    22501 atgatcctct cggtgcatgg aatttatatt ccttgccggg aaatccgaag gataataatc
    22561 ggtggactga ttttccgatg ttcgcattga cagaaaatga attatttctc accattaacc
    22621 tcatcattcc cgatgagcct tggcaaacag gatttagtga aacgttgatt tggcaaatgt
    22681 cgctcgacaa aggctatgcc ggcgatacac ttgatgccgt gtattatgac agcatttttt
    22741 ttgaaggcaa accattgcgc aatctcaatc ctgtaaaagg cggcgcaaat acatacggac
    22801 cggatatgtt tttcctgagc aatagaaatt tcgatcctgc aaatgacact atttttatcg
    22861 tgcatgttac cggtacttta gatgatccgg aaactgaact gacagtagat tttggaagaa
    22921 gtaatattgc ttacggtgtt ccgcctcagg cgcgacaatt ttcgacgcat atttttgata
    22981 cgaatgacgg tcgcgtgctt ggaagttttt atgaaaacaa ccaaattcaa tttgtgtcca
    23041 acaccctcga tccggcaacc ggattttgcg gtgtttacca cggcgtaatc actgatccta
    23101 caggggcaaa aactataacc gcaaatatca ttggtgatga caccctggat ctcggatatc
    23161 cgaatatttc ttactccgga aaatttaccg gcgatgatca atcgattatc acctgcgagc
    23221 atacagcgcc aacagtttat gcaggggtaa gcggaatatt ttttaattgg ggcggttatt
    23281 cagatttaat taatatccat accggtgata cctacgtgaa tattctcagc ggagcatacg
    23341 aacgctgggg cgattacacc ggatcgcaac gcaaatacga tgaacctgga gtagtgtgga
    23401 tgagcggaac ttttggaaaa tatattgatg tatttccttt tgtgcaacgt aataatgcta
    23461 catggattgc atctctgcgt agtacggttg agcctgcagt taatgttcct gtaaattcaa
    23521 atatgtcacc cgcaagatta tttccaaatc caacagcaga tgtgtttact acggagatca
    23581 atttgagtat tgcaggtatt attgaatgtt acttgtatga tatgcagggc cgccttgtta
    23641 agtcactgct tcgcgctgaa ggaaaagccg gtaacaatca ttttcagttc agcatggcac
    23701 cactatccac cggaacatat caactataca ttaccctcaa tagtactgct cttactaccc
    23761 tccccatcat aaaaaactaa tcccccaaaa aaactctgcg tctctgcgtc tctgcgtgaa
    23821 ccaaacattg tcatcatggc ccgtaacaaa aagaaaatcc atcaacatgt attaattgaa
    23881 tccgttgcca acagcggttc ggcattcggt agggtagaag ggaaggtggt attcaccgaa
    23941 ggcgtaattc cgggcgatat tgtagatatc caagtagccc gcaagcgcaa tggatatgat
    24001 accggatacg taacagctat tcataaaaag agtgatttac gcatagagcc cttttgtacg
    24061 catttcgata tttgcggcgg ttgtacttgg caaaatgttg aatacgaaac gcaattacaa
    24121 ttcaaacagc aattggttga tgatgccttt cgcttgttga aacgctctcc cggatttcct
    24181 gaaatagaga aaattatcgc atcgccttat gatcggtatt accgcaataa aatggaatat
    24241 actttcacca atggcgctta tatttttgat cctcattatg ataagtcaaa tccccctgaa
    24301 aaaaagccgg gttgtggttt tcatgtgaaa ggtcagtttg ccaatgtgtt tgatatggaa
    24361 cgctgttatc tccagggatc gctttccaat gagatcagaa tggctgtgcg ttcctatgcc
    24421 ttgatgcatg gttttgcatt ttacgatttg cgcaatcata caggatatat gcgcaacctc
    24481 atcattagaa atacaggcac cggcgattta atggtgatac ttgttgtagc acttgatgaa
    24541 ccggaaaagc tgctgccgat gatggaacat attcgcagca catttccgga gatcacttcg
    24601 ctgtactatt gtatcaatac caagcacaat gatgttattt atgacatcga tgtaacatta
    24661 tttgccggca aaccacacat gattgaggaa attgacggca tcaaatatca actcggtccg
    24721 cgcgcattct tccagacaaa taccgaacag gcaaaaaatc tttacaggaa aacgattgca
    24781 tatgcagctt tgaaaccgga cgataaggta tatgatttgt ataccggttt gggaagcatt
    24841 gctttgcaag ctgctaataa gtgctcaaaa gtagttggag tagaacttgt tgaagccgct
    24901 attgccgatg cgaaagaaaa tgcaaagctg aataatatcg ataactgcac tttcatagca
    24961 ggcgatatgg ccaaaatatt taccgacgat tttattgcgg agcatggcaa agctaatgta
    25021 gtcatcacag atccgccaag agcaggcatg cataaagatg tttgccttca gctattgaaa
    25081 ctggctcctg aaagaatcgt gtatgtgtcg tgtaatccgg ttacacaggc tcgcgacata
    25141 caattgctta tcgatgatta caaggtgacg gcgcttcaac cattcgatca atttccgcaa
    25201 acgtatcacg ttgaaagcat tgccctttta gaaagaaggt aaaattcacc caactgtaga
    25261 tttagaaaac gaaggtagca gcaaattagg gacagcctaa aaaagtgaat gctgcacgct
    25321 cctcatcgta cagcattctt taggggtatg ttgcctaacc aataacctta aaaggttata
    25381 acaaagataa aaattagcga ttgatatttg caaataaaat aataataatt tagcaaaatt
    25441 gggtttttat aaaaatacat ccattatatt tgcataataa gcaatcgaac agccaagcca
    25501 tgtcagaaaa ttacgaaatt gataaagtag acattcaaat cctccatctt ttgatgcagg
    25561 acgcaatgac tccttttacc gaaattgccc agaaaatagg ggtttcgccg ggaaccatcc
    25621 atgtgaggat gaagaaaatg gatgccatgg gactcatcaa aagctcccat ttgatggtga
    25681 atccacagaa actgggttat ggtataaccg ctttcctagg ggtttatcta gataaaagtt
    25741 ctatgtacga cagtgtagtg aaggagctcg aaaagatccc tgagattgtc gattgtcact
    25801 acatgaccgg aacctatagc atgtttctca agatcatttg taaggatacc aatcatctga
    25861 aaaaggtgct tcatgatggt attcagccaa tcaatggcat tgacaggaca gaaacttcta
    25921 tttcattgga agaatctttc agcagaccaa tgaagatcga attgaacgga aggaaggatt
    25981 tttaactgtt tgtcggtaga aaattatggg ctgtgtttgg gtttcaactt gcccaaattc
    26041 ttcggtcgag atcaaataac tcgggaacag ttactaatgc attcaacatg gcatattgct
    26101 tttcagccag cgttttgttg taagtatttc ctatcgttgg cggtgaaaaa atatcctttt
    26161 cacttttcac ggcaacaaaa acgcgattgc cgacaaacga taaatacacg tcatcaacaa
    26221 aataagtttt cagctccata atgcgatgaa ttaatttcgt tgagagaata taccgtgctt
    26281 cctgctgatc agtagtatac acagaaaatg ctttttcaaa atcaggatct tccattcttg
    26341 caatgctcgt tccggggcga tgaaaaagtt catttaatga ttcaccatac acttcaccaa
    26401 gtaattcccg cgaagcatcc ggtgccacga atgtttcacc ggtaaaagat ttgttgaatg
    26461 ctgccgataa taaatatcct ttgaataaag tagaatattt agtttctgac ttgccgttag
    26521 attgggtgcg ttccacttgc ataacatcaa gaaaactaca ttgaaattgt tcttgtgagc
    26581 gtgtgatgag gtcactgcct ttcaaggatg aaatttcctg ataaggaaat aatttacttt
    26641 ccgtaaatgt ctgggtgccg atatgttcat tcatcacata cctggcttgc gctgcaattc
    26701 cgggtaaaaa attggcaaat aaaatggatt tatattgttg tgtgaagtta tcttcaatac
    26761 gcttcagcaa catatatggt cccaacaaaa aaaatatcga accgatagcg ccggcccatt
    26821 taatcataaa tgttggtgaa aaggtgcctc ctaaaaaagc agtgcctgca aagtatccta
    26881 tcgcaaacgc gcaaactgta aatacaatag tgattagatt caatgtccaa ggctgatgag
    26941 cgatagcgct ttgcatactc aaaaacctgt cgcgggcagt tttgcggata ataccaaaaa
    27001 gtaccatcgc caaaacaaac aacataaatc ctctgatgac acgaaaaaac atatcatcgc
    27061 ccatttcgcc attcgacatc atggatagaa acccgataaa catgctgact atgagtgcaa
    27121 cgaagaagaa ctcaagtatc atgcgcaata cccttgacat ggtatgctgc cattttttgc
    27181 tatcgagtgg tgatagtgct gtttcgagtt ttaataatgt ttcatccgtc aatgccatga
    27241 aacaaaacta ggtcattttg caattacaag tatatcggag atatgtgtat tgttgttgct
    27301 taataccaaa ttatacatgc cggatgcgag gttcttcaca tcaacagtat agtcatcgcc
    27361 tgaaaatagt atggtttgct caactacctt ctggccttgt aaattgatga tatacatttg
    27421 atatacgcca tcggcaattc cctcagctga taccgtgcaa ttttcttcgg caggattagg
    27481 gtaaataacc aacgagctct ccgactcgga agttatttcg ccttcgcgca atggaagggt
    27541 agtgaaattt accaaagctg aaaacccact ggcagggtag gggtcgcatt gagtttttac
    27601 cttgtattcg taggtggtgt ttgcagctaa tccggttaat ttctttgaat tggtggtagc
    27661 agttttctta atccaggttg caccgcctac aggccgataa taaatctgat attttgttgc
    27721 cccgggaaca gcagtccagt ttactcttgc actgtttgaa gtaatggaat tggtaaacaa
    27781 tcctgttggt gctgcacaag tgaatgtgtt tacagtcaaa ttagtaatga tggttgaatc
    27841 acacccaagc gaagtgttaa aattgctcgt atatgtgcca cttgtgctca ccacacttcc
    27901 attcggaagc gtgtatgttg caggtgcagt gatagtcaca ttttctgtaa aactgtaagg
    27961 aatgcaactc aatccgctta ccactatcgt ataatcctga tctcctcctg ataatgttcc
    28021 tttgtggttg atgctgattt tataatctcc agcacttggt gttgccagat atacatattc
    28081 aacattgtcg gtattattat cacctgttgt agctgc
//
LOCUS       DAOQHY010000016        27614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_747_length_27614_cov_4.932073, whole genome shotgun sequence.
ACCESSION   DAOQHY010000016 DAOQHY010000000
VERSION     DAOQHY010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 27614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27614
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_747_length_27614_cov_4.932073"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PLL28_02520"
     CDS             <1..368
                     /locus_tag="PLL28_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HNF68216.1"
                     /translation="NVLLLGEQYALSLGIHVPRLRLYIIVITGILTGTVTAFCGPIAF
                     IGIAIPHLARMVLKVNDHRLLIPASVLFGMCVMLLCDMLTQVGGDAGVLPLNAVTAFM
                     GAPFVIYIIYKNQHLKRTL"
     gene            365..1147
                     /locus_tag="PLL28_02525"
     CDS             365..1147
                     /locus_tag="PLL28_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF68217.1"
                     /translation="MTILIQHIIKSTALCVGYDKHVILKDINIAVPQNSVTSIVGLNG
                     TGKSTLLRSLCGLQKILSGEVLLHNKSLSDFSAGEIAKQISIVLTGRGEMVSSLSVYE
                     LLAMSRTPYLHWLSGVSGHDIAIIEESAKVAGVSHLIDRSLYTLSDGEFQLVGIARAL
                     AQETPIIILDEPTAHLDMQNKLEVFSLLRKLSKSGKTILFTSHDIDLALAISDHVLFI
                     DKKGKVEFGETSSLIERNIPATYFDSANIQFDKETNRFKFLL"
     gene            complement(1139..1693)
                     /locus_tag="PLL28_02530"
     CDS             complement(1139..1693)
                     /locus_tag="PLL28_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF68218.1"
                     /translation="MYFHSIGRPFVGKKISAVLPFLPKDVPVLDIGSGNGLAASLMME
                     AGCKIVPIDIHEGQYHDSVKPTVYDGKHLPYVDNHFEAGVILTVLHHVDDPDALLKEI
                     MRVCKSLVIMEDIYENAFQKNITFWLDSLANLWYSPCPHTNKNDIGWKSLFNAMDLEL
                     TDVRYRNAFFVIKQAVYVVSKKSQ"
     gene            complement(1760..2299)
                     /gene="msrB"
                     /locus_tag="PLL28_02535"
     CDS             complement(1760..2299)
                     /gene="msrB"
                     /locus_tag="PLL28_02535"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNF68219.1"
                     /translation="MRTLIVVIALMPAIFGCSQNTQKATETNSSIETLDNKVTTESAD
                     YVFDGKIITKPDSVWQKELNGMTYYVMRQAGTERPFQNAYYDNHEKGIYYCAACGLPL
                     FSSETKFDSGTGWPSYYTPINDLNIGKDTDLDIGYVRTEVHCARCDGHLGHVFDDAPQ
                     TPTGLRYCINSASLVFKKQ"
     gene            complement(2392..4023)
                     /locus_tag="PLL28_02540"
     CDS             complement(2392..4023)
                     /locus_tag="PLL28_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNF68220.1"
                     /translation="MITVNNVSLRYGKRLLFEDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILAGEIEPTTGLIDITPGERMAVLKQNHNEFDQHSVIHTVMMGHRKLWNIIQEKDTL
                     YAKPDFNEEDGIRVGELEAEFAEMNGWNAESDAAELLSNLGVKEDLHYVPMEQISGKE
                     KVRVLIAQALFGNPDILLMDEPTNDLDIDTISWLEDFLADYENTVIVVSHDRHFLDAV
                     CTHVADIDYGKINIFTGNYSFWYQSSQLMLQLMAQKNKKVEDKRRELQEFISRFSANA
                     SKAKQATSRKKALEKLNVEEIRASSRKYPGIFFTPERDLGDQVLQVTNVSKSLEGRNL
                     FSNVNFTAEKGDKIAIVSRYPIAKTYFFEIIAGERQMDQGEFKWGVTVTSNYLPTDNS
                     SFFAGEMNLIDWLRQYSKEKDESYIRGFLGRMLFSGEESLKSCKVLSGGEKVRCMISR
                     AMLLGGNALIMDEPTNHLDLESIEALNNALADFKGFVIFTSHDHQFMQTIANRVIEIT
                     PNGIIDRLMTYDEYILDPKVKELQDQMYPSSVTAE"
     gene            complement(4120..4764)
                     /locus_tag="PLL28_02545"
     CDS             complement(4120..4764)
                     /locus_tag="PLL28_02545"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNF68221.1"
                     /translation="MAGKQNKLFLYGTLVVAALVVFAIFMVYNNTMSGNKELELPFYG
                     PRGSNGEPHYVQDFSLLNQDSVLLDMTPYKGKIYVADFFFTTCQGICPIMTEGMLKVY
                     HAYETTPNVMFLSHTVDPDHDTPYILKRYAGNRDINNAKWQFVTGDKKQIYDLARYSY
                     LVDASVGDGGPTDFVHTENFALVDPEGRIRGYYDGTDTVEVNKLIKDISLLMAE"
     gene            4892..6124
                     /locus_tag="PLL28_02550"
     CDS             4892..6124
                     /locus_tag="PLL28_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNF68222.1"
                     /translation="MQKSNRLTLYILAAMVLGIFVGSMIHNSGKSDEQLGAIASKINI
                     TTTVFLSLIKMIIAPLVLSTLIVGIAKMGDVKTVGRVGGKTMLWFITATIISLTLGMF
                     LVNALQPGEAMNLALPAETASAGVDAGAITLEKFITHVFPTSIFKAMSENEILQIVVF
                     SIFFGVACAAIGEKAAPLIKALDIVGHVMIKITTYVMLFAPFAVFAAMCSVITTKGTS
                     VLLTYGIFMRDFYFGLLILWAILIIAGFAVLRKRVFILLRRIKEPFLLAFSTASSEAA
                     FPKLITELERFGCNNKIVSFTLPLGYSFNLDGSMMYMTFASIFIAQCYNIDLTMSQEI
                     TMLLTLMVTSKGIAAVPRASLVVIAGTISMFNIPQAGLVLLLGIDTFLDMGRSATNVI
                     GNAIATAAVSKWEKALED"
     gene            6164..8083
                     /gene="acs"
                     /locus_tag="PLL28_02555"
     CDS             6164..8083
                     /gene="acs"
                     /locus_tag="PLL28_02555"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNF68223.1"
                     /translation="MAVPYQITSAEAYQHAYAKSIENPAAFWADIASHFTWKKQWELT
                     LDWNFVEPHVRWFLGGQLNITENCIDRHLESLGNKPAIIWEPNDPEESHRVLTYRQLH
                     DKVCQFAHVLKNNGVEKGDRVCIYMGMIPELAIAVLACARVGAIHSVIFGGFSAQSIA
                     DRLQDAQAHFIITCDGAFRGAKDIPLKSVIDDALIACPFVERVIVCTRTRTPVSMLKG
                     RDVWWEDEIKKVETLGLHNFEPEVMESEDPLFILYTSGSTGKPKGVVHTTAGYMVYAN
                     YSFVNVFQYQPEEIHFCTADIGWITGHSYIVYGPLSAGATSLMFEGIPTWPDAGRFWD
                     IVEKYKVNILYTAPTAIRSLMGFGTAPIEGKDLSSLRVLGTVGEPINEEAWQWYHKDI
                     GKENCPIVDTWWQTETGGILISNLAGITEAKPSYATYPLPGIDPILVDENGNIIEGNN
                     VSGNLCIRHPWPGMLRTTYGDHERCRQNYFATYHQLYFTGDGALRDEAGRYRITGRVD
                     DVLNVSGHRIGTAEVENAINMHAGVVESAVVGYPHDIKGQGIYAFVVYTGGHQSDPQL
                     TRQDILLTVTRAIGALAKPDKIQFVSGLPKTRSGKIMRRILRKIAEGEPGQIGDTTTL
                     LDPNIVEEIKSGVIN"
     gene            complement(8084..9034)
                     /locus_tag="PLL28_02560"
     CDS             complement(8084..9034)
                     /locus_tag="PLL28_02560"
                     /inference="COORDINATES: protein motif:HMM:NF018659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HNF68224.1"
                     /translation="MVTTPHNIKYQLGLTVNRALWVFSAATLLLLNSCAVLLPKEYRT
                     VGYNLEEPGFRKLLPPSLQEISGITQISMDTFACIQDEQGIVFIIDVTTGNIIKQMPF
                     FGDGDYEGIARVNNSLFILRSDGLLFEIPDFHSPEYGHITFKTGIPANNNEGLCYDPE
                     HNRLLIACKSNIGKGATLKNVRAIYAFDLGTKKLSSDPVFEVYISELQDYAKTHAMDL
                     PEKKSKHKKKQEQPDIKFRPSAIAIHPENHNIYLLSAVDHLLCIVNPNGSLVSLISLD
                     ENVFNKPEGIAFSSDNTMIITNEGQDKHPSLLSFPYKGRR"
     gene            9017..10288
                     /locus_tag="PLL28_02565"
     CDS             9017..10288
                     /locus_tag="PLL28_02565"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HNF68225.1"
                     /translation="MRSRNHIPYLQIYNFAPQHLKMSEKRQASMIFIFITMLIDVVGF
                     GIIIPVLPNLIKELHGGDYSEAARIGGWLLFAFSLMQFLFSPVLGNLSNQVGRRPVLL
                     FSLFGFGIDYLIMAFAPSLWWLVVGRVLAGITGASFSTASAYIADISTPEKRAQNFGL
                     VGAAFGMGFIIGPALGGLLAGLGPRVPFYGAAILSLVNWLYGFFILPESLPKEKRRKF
                     EWKRANPIGSLAALKKYPLTYGLIASMIFIWLASHAVQSNWSYFTMGTFHWDEKAVGF
                     SLALVGVLVGLVQGLLIRVTIPRFGQKNSLYYGLLLYTIGLLLFAFATQGWMMYAFLV
                     PYCLGGIGMPALQGIMSSQVPANEQGELQGAMTSLMSVTSIIGPPLMTNTYAYFTAKD
                     TPYFFPGASFLLGAIFMAVSLLLTMRTLKKH"
     gene            complement(10289..10738)
                     /locus_tag="PLL28_02570"
     CDS             complement(10289..10738)
                     /locus_tag="PLL28_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68226.1"
                     /translation="MRLPPVILGFFALGLTLIACRQDDAVYLQAPENKYILCRGSALY
                     LDNGKRWKSNIETTEGIDSMIVQLNKFPDDADSAAYQALGSELVEDLIYIVNHCEQMG
                     NNHEMVHAYLNPLQELIVPLQVSGTETCKAQLPKIKDHLSKYHEYFE"
     gene            10865..11812
                     /locus_tag="PLL28_02575"
     CDS             10865..11812
                     /locus_tag="PLL28_02575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNF68227.1"
                     /translation="MNDNSLLWLGFNAFVLLMLALDLGVFHRKSHVVGIKEALAWSGV
                     WIALALVFNGLIYFGIVGGYEGAELQKQKGMEFLTGYLIEKSLSIDNIFVFLLIFTFF
                     KVPQLYQHKVLFWGIIGALVMRAIFIFAGVALIEKFHWIIYVFGVFLIITGINMVRNK
                     NKQLDPEKSLVIKGFRKIFPVHNSYDGSKFFVTLNGKRHATLLFVVLVFIEFTDLIFA
                     VDSIPAILAITPDPFIVYTSNVFAILGLRSLYFALAGIMHRFAYLVYGLAAVLVFVGL
                     KMVFVDVYKIPVQYSLLVIAILLAASIFYSIYKTRKTKA"
     gene            complement(11823..13028)
                     /locus_tag="PLL28_02580"
     CDS             complement(11823..13028)
                     /locus_tag="PLL28_02580"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF68228.1"
                     /translation="MFGKALNIYLDSYRGINRSVWILAAIQLINRSGSMVLPFMTVYM
                     TANLGYSTVQAGIIMSVYGLGGLLGSYLGGRWTDKFGPFQVQLFSLLFGGGCYLLLPL
                     LTGFYSLALGIFVCATVNDAMRPAASSMIGHFATKETTTRSFSLMRMAINLGVAIGPA
                     VAGLLAETNYFWLFFGDGITCIAAGVVFFFYFRGKRPQIHKEDKQEDQPQKSPYTEPA
                     FLYFLFLCFCYAAAFFQIFSSIPLYYKDVYFQSEKAIGLLIGFNGVIVFLFEMITVSQ
                     IEKRFKPERLIIAGTLMLGVSFAIFNLFTGMWVLITAMTLLSFSEIFAMPFMISHVVK
                     TATPQTRGSHLAAYTIAWSVAFIVSPYCSTQIIDKLGFDALWWTFGIFCAIIAIGFLK
                     VRGIKSDVE"
     gene            complement(13088..13657)
                     /locus_tag="PLL28_02585"
     CDS             complement(13088..13657)
                     /locus_tag="PLL28_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HNF68229.1"
                     /translation="MRKRKIAVGITGASGAIYAKVLLDKLQLLGAEIEKVGVVMSDNA
                     KDVWRFELDNEQFTNYPFTFYGKNDFMAPFASGSAWFDTLIVCPCSMGTLGRIASGIS
                     NDLLTRTADVMLKERRKLILAVRETPYNLIHIRNMETVTTAGGIICPLSPSFYSKPKS
                     QEEVAATVVDRILQLADFSVPMYRWNDPS"
     gene            13736..14374
                     /locus_tag="PLL28_02590"
     CDS             13736..14374
                     /locus_tag="PLL28_02590"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNF68230.1"
                     /translation="MQKRILISAALIMLLGAALMAFVPMDTLKEASGADLMMATTVED
                     SAKTEFKWYTISEALEMQKTSHKKIFMDVYTTWCGPCRMLDANTFSNPEIQKLLSEYY
                     IPVKFNAESGDTIQYQGQTFTNRNYSPLPRKSTHDFAVYIASTEQGLGYPTVVFMDET
                     GTRLQPISGYREAGKGTAQNPGMEPILSFFGTDAYKTATWNDFYAAFQSNIK"
     gene            14378..14968
                     /locus_tag="PLL28_02595"
     CDS             14378..14968
                     /locus_tag="PLL28_02595"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HNF68231.1"
                     /translation="MFIEIALSLVMFFHEDHQETIAPAAEQAGVDTIGWMSLSDVNKI
                     VEKNIKKGKVKEDKLVIVDFYTDWCGWCKKLDAETYKDPEVAALMNKYFYPVKFDAES
                     KDTVAFANKTYGFKSSGSRGTNEFALEMASRPGGRIGYPTITIINALGEKLTVEAGYK
                     NPEKMKLMLLYYGEGHYKTKDFNTFVTEYNQKQLTD"
     gene            15065..17014
                     /locus_tag="PLL28_02600"
     CDS             15065..17014
                     /locus_tag="PLL28_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNF68232.1"
                     /translation="MSSAAKNIHKLSAAGVLISLGIIFGDIGTSPIYVISAIVGDHVI
                     SRELILGGLSCVFWTLVIITTFKYVILALNNDNRGEGGIFALYAIHRRFKVKWAIVPA
                     LVGCAALIADGFITPPISISSAIEGLKLLNPNIDSVVPIVIIAMIILFTVQQFGTNVV
                     GKAFGPVMSIWFLMIGILGSIQVVQHPEVLEALNPYWAIHLLTEYKGGFWLLGSVFLC
                     TTGAEAMYSDLGHCGKQNIRFSWTFVLTMLLLNYAGQSAYMLTMDGQQLNDHSPFYMI
                     MPTWFLPIGIIVATSATMIASQAILTGCFTLVNEAMKLKLWTNFKVVYPSTVKGQIYI
                     PAINWFLFAGCVTVVLLFREAKHMEAAYGLAITFDMIMTTVLLTSLMLLHKRAKLMVF
                     SLAALFLLIEGSFLVSNLNKFTHGGWFTVSLAVGIFFALWIFYEARKLRAKHTEFVEI
                     DKYLPAIQDLMHDESIAKEATHLVYLSMVNDKNHIDSNIIYSIFRKKPKRADVYWFVH
                     VDISNDPYLATYSVDTIVPKKIFFVRLRFGFKVEHKVNVMFRRVVEEMVAAGEVDLLS
                     NYPSLRKHGMYADFKFILLNSRVSIDNKLSPWEQIVIKGYRFFKSLSLPTHEDFGLEL
                     TNVEYEMVPIKVSQEIEIKLERVTK"
     gene            complement(17004..17747)
                     /locus_tag="PLL28_02605"
     CDS             complement(17004..17747)
                     /locus_tag="PLL28_02605"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNF68233.1"
                     /translation="MRRFAISDIHGCHQTFSALLDTLQLTKEDELYILGDLIDRGPNS
                     IAVIDQVLKMVEEGYKVTTLMGNHEWMFMQALDETIPYCWDWRRYQAWVKNGGVTTMM
                     NLGYKRIDDLKNIDKKYDTWVRSLKPYIELDKVILVHAGFNFKEEDIFKDTHSLYWIR
                     NYYDTIIPEKINNKIIVHGHTSRPFNTLEQELSEMKYPAVNIDCGCVYVGRPGYGHLC
                     ALDLDTMRPIFQKNVDRIEKPVASEESTL"
     gene            complement(17752..20259)
                     /locus_tag="PLL28_02610"
     CDS             complement(17752..20259)
                     /locus_tag="PLL28_02610"
                     /inference="COORDINATES: protein motif:HMM:NF036809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68234.1"
                     /translation="MKSIHLLILACSMLHFSVHAQMESITLDQQWQFRRFGDTQWMKA
                     KVPGCVHTDLLTNNAIPDPFIDTNEAAVQWVENEDWEYRTTFHLHKNQLNSDFIDLNF
                     GGLDTYADVYLNDSLILSSTNMFRAYKVPVKTLLKSGDNLLFIHFYSPVKRANEVGEQ
                     FTVPLPSDERVYIRKAQYQFGWDWGPRLVTSGIWQPITLVFNDKLKIKFVDFYTEQNP
                     DYQFPVHIDLAYEALGRMHYEVEVKELTTGKTCYSWSYRDQLPTVEQINFNFHAPEVW
                     MPYALGSQNIYAFELKITHNKRIYYQQVFKTGFSNIQLHQEADASGKSFYFTANGKKV
                     FINGANFIPAHSFPTEASDERYRDILIEAKRMHINMIRVWGGGIYEQDIFYDLCDSLG
                     IMVWQDFMFACAMYPNDVTEYPEFQQQITRLKNHPCLALWCGNNEIDEGWNNWGWQKQ
                     LKYNDDDSAYVASLNEKLFRLVIPEYLHSNAGAYQSYHPSSPTYGWGRKESLTSGDAH
                     YWGVWWGKQPFDIYNKKVPRFMSEYGFQGIPSYHSWEKCINSDNLYLGSPAFKAHQKH
                     PVGYETIDEYMKRDYGTPSDLQSYIYISQLLQADGMRVAIEAHRRNMPYCMGTMFWQL
                     NDCWPVTSWSVQDFYGNRKAAWYTVESCYRDVIISVLQEGDELQTWLVNNDTTDRKLQ
                     LTYSLLTTSGVTIRNQTLPVTLKANTSMLYSTLGVEWFLSGYAKENCILWLSLSEEDK
                     VIAERMHTFVRPGAVSYKKADIAISIDGDVITLTSPVFARQVYLYSDDTELRLSNNFV
                     DLLPNTPKQISLQDISEGTIPDVSKLKVLCLNNLKLN"
     gene            complement(20256..21014)
                     /locus_tag="PLL28_02615"
     CDS             complement(20256..21014)
                     /locus_tag="PLL28_02615"
                     /inference="COORDINATES: protein motif:HMM:NF015866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HNF68235.1"
                     /translation="MKTSVTRKYILEICTFNATDVVHAVAAGADRIELCASYVEGGIT
                     PSSAAITAAMQMIDPENIVVMIRPRGGDFMYDDFEFDVMRHDILHCRSLGVKQVIFGI
                     TTPDGKLDVERNSRLIDTASGMQCTLQRAFDLTRDPFEALEDAITCGFTRILTSGQAN
                     TAIEGKEIIKQLITSANNNITLLPGAGVNSSNAREIIDYTGCNEIHTSAKRPFTPKST
                     SYNFNSNIYDTSHLTEVNPEEVHKLSAIIATYNS"
     gene            complement(21015..22010)
                     /locus_tag="PLL28_02620"
     CDS             complement(21015..22010)
                     /locus_tag="PLL28_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HNF68236.1"
                     /translation="MTSRRSFIKTAALGAGVISTGQSLQSAVVENQHKSVPMNKPLVL
                     STWDFGLKANVDAWAILSAKGRALDAVEAGVKVAENDLHSLSVGLAGLPDREGHTTLD
                     ACIMDEHYNCGSVAFLEKIKNPISVARMVMEKTPHIMLVGSGAQQFALENGFTLDDYK
                     NPEAQKAYEAWLVEKQYKPVINIENHDTIGMIAMDMQGNLSGACTTSGLAYKVRGRVG
                     DSPIIGAGLYVDNEIGAATATGTGEEVIRIAGTHLVVELMRQKYSPYEACKEAVMRMV
                     KIRKEKTKDFQVGFIALNKAGEFGAYCLQPGFNYAVFSNNIANELFKSDSYYPEQ"
     gene            complement(22089..24386)
                     /locus_tag="PLL28_02625"
     CDS             complement(22089..24386)
                     /locus_tag="PLL28_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HNF68237.1"
                     /translation="MKTTLIFTLLLTLHVNMLKAQQCPIVPKPVEYTANPGYFYFKPT
                     SYILVNDMASFMDAVTFRDMSIKFHSLPLKTVREEIYPSHYIEVSYDSTLMIPDGGYT
                     MLITEEKITIIGKNKGGVFYGLMSLLQMTESKFTNQFQVPCAGISDFPQFQYRGMHLD
                     CSRHFFTVDEVKMYIDYLALYKMNTFHWHLTDDQGWRIEIKQYPKLTSVGAYRTGTMK
                     GHYRDQQYDTINYGGYYTQKEISEVVKYAKNRNITIIPEIEMPGHCAAALAAYPELGC
                     IADTTYSVGQAWGVYKEAFCPKPETFEFLYRVLDEVMALFPSQYIHIGGDEVEKDQWM
                     HCDYCQQLIKEKNLVDEHGLQGYFTKTIAEYVQSKGRNIIGWDEILTNDLPQQAAIMS
                     WRGVNGGIDAARAGHYAVMTPGSHCYFDYYQGLPYQEPLAIGGYIPLQKVYEYHPIPD
                     ALTPEESKYILGAQGNLWTEYIPNFDQVEYMVIPRMLALSEVLWTPDTARNYADFMRR
                     LSTQFELLGRVDCHYSKSAFGIIPEVLPTDDFNGVRIALRTWDNTTGFEYQYEDTSKN
                     SPAVFGGAFIKGIYTAPFIVTESYSLIIPEDYKHPEVKLRLLFNKATGKKITLKTPPA
                     QQYSGSGAFSLVDGINASLYPSWSGNEWLGFSGTNLNCTIDLGAVDTIMAVSAGFLED
                     KTSWIYLPSSMEISVSLDGKKFTRIGIVPVVKDDRAITNGIYKFDPTAARYVRIIANN
                     IGTIPQGNPGAGNKAWLFVDEVHIR"
     gene            complement(24396..26039)
                     /locus_tag="PLL28_02630"
     CDS             complement(24396..26039)
                     /locus_tag="PLL28_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNF68238.1"
                     /translation="MTKETQNIAFNRNEDALRLLVSTMKQRLTKVQEGGGKKSIDKLH
                     EKGKLHARERIKLLFDMDTPCIEIGAFTAMGMYTEHGGCPSGGVVVMIGYVSGRQCIV
                     VANDATVKAGAWFPITGKKNLRAQEISIENKLPIIYLVDSAGVYLPMQDEIFPDKENF
                     GRIFRNNAVMSSMGITQIAAIMGSCVAGGAYLPIMSDEAYIVKNTGSIFLAGPYLVKA
                     AIGEDIDAETLGGAITTTEISGICDYAAENDEDCITHIRSTMDKIGAMPKAGFDRIAP
                     SAPSLKEEDIYGIMPEDITKQYDVLDIIKRLVDNSVFDEYKAAYGKTIVCGTARIDGW
                     SVGIVANQRKVVKTKKGEIQIGGVIYNDSADKAARFIMNCNQKKIPLLFLQDVTGFMV
                     GSKSEHAGIIKDGAKLVNAVANSVVPKFTVIIGNSFGAGNYAMCGKAYDPRLIVAWPN
                     AKIAVMGGAQAGKVLLQIQVASLKAKGEEITAEKEKALLEKILNHYNETMDPYYAAAR
                     LWIDGIIDPLDTRKWVSMGIEMANNAPIEKDFSVGVMQV"
     gene            26304..26960
                     /gene="fsa"
                     /locus_tag="PLL28_02635"
     CDS             26304..26960
                     /gene="fsa"
                     /locus_tag="PLL28_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HNF68239.1"
                     /translation="MKFFIDTADLNEIKEAASLGILDGVTTNPSLMAKIGITGAENIK
                     QHYIDICNIVDGDISAEVISTDFEGMVREAKELVALHPNIVVKIPMIKDGVKALKWCT
                     DNNIRTNCTLVFSAGQAILAAKAGATYLSPFIGRLDDINWDGVQLIEQIVHIYRNYGY
                     ETQVLAASIRNPLHIIRCAEVGADVVTCPLSSIMGLFKHPLTDIGLAQFLADYKKGNG
                     "
     gene            27110..>27614
                     /locus_tag="PLL28_02640"
     CDS             27110..>27614
                     /locus_tag="PLL28_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNF68240.1"
                     /translation="MHRLPSDEVLPFLLMVATLLLAARLMGELFRKLKLPQVMGELVA
                     GILLGPSCLERCAPGFYESFFASPENAGVAYDGLARIGILLLLFVAGMEINLKTIQKK
                     ANAAAAISLSGVAFPFLIGFVASWYLSSYLFPQGMGDKLASSLFMGTALSITALSVLA
                     KILIDIDK"
BASE COUNT         7784 a         5655 c         5683 g         8492 t
ORIGIN      
        1 tgaatgtgct cttgctgggt gaacaatacg ctttgagtct tggcatacat gtgccccgct
       61 tacgattgta tataattgtt attacaggca ttttaaccgg tacggttacc gctttttgcg
      121 gaccaattgc atttataggt atcgcgatcc cgcatttggc gcggatggta ttaaaggtaa
      181 atgatcatcg cctgttgatt ccggctagtg tattgtttgg aatgtgtgta atgctgcttt
      241 gcgatatgct tactcaagtg ggaggtgatg caggtgtgct cccacttaat gcagttacag
      301 cctttatggg tgcgccattt gtaatttata ttatttataa aaatcagcat ttgaaacgta
      361 cgctgtgacg atattgatac aacatattat taagtcaaca gcactttgtg ttggttatga
      421 taaacatgtc atcctcaagg atataaatat tgctgttcca cagaatagtg tcacttcaat
      481 tgtaggctta aacggaaccg gaaaatcgac acttttgcgc agcttgtgtg ggttgcagaa
      541 aatattgagt ggtgaagttt tactgcataa taaatccttg tctgacttta gtgcaggtga
      601 aatcgcgaag cagataagta ttgttttgac aggtaggggg gaaatggttt catcactgag
      661 cgtttatgaa ttactggcga tgtcgcgcac accttatttg cattggttgt caggtgtttc
      721 aggtcatgat attgcaataa ttgaagaatc cgctaaagtt gcgggcgttt ctcatttaat
      781 tgatcgtagt ttatatactt tgagcgatgg tgaatttcaa ttggtaggta ttgcaagggc
      841 actcgctcaa gagacaccaa tcatcatact tgatgagcca accgcccacc ttgatatgca
      901 gaataaactt gaggtgtttt cacttttgcg taaattatct aaatcaggta aaacaatttt
      961 gtttacctcg catgatatag atcttgctct ggcaatttca gatcatgtat tgtttatcga
     1021 caaaaagggt aaggttgagt ttggagaaac gagttcattg attgaaagaa atataccggc
     1081 aacctatttc gattctgcta atattcaatt cgacaaagag acgaatagat ttaaatttct
     1141 attgtgattt tttgctcact acgtatactg cttgctttat cacaaaaaat gcgtttcgat
     1201 accttacatc tgtaagttca aggtccattg cattaaaaag tgatttccag cctatatcat
     1261 ttttattggt atgtggacaa ggagaatacc acaaatttgc aagtgagtct aaccagaaag
     1321 tgatgttctt ttgaaacgca ttttcgtaga tatcctccat aatgacaagt gatttacaaa
     1381 cacgcataat ttccttcagc agagcgtcag gatcatcaac atgatgcaag acagttaaaa
     1441 ttacgccggc ttcgaaatga ttatcgacat aaggcagatg cttgccatca tagactgttg
     1501 gtttgacact atcgtgatat tgcccttcgt gtatatcgat cggaacaatt ttgcatcctg
     1561 cctccatcat aaggcttgct gctaatccat tgcctgatcc gatatctaaa accggaacat
     1621 ccttaggaag aaagggtaaa actgcactga ttttttttcc tacgaatggc cttccgatcg
     1681 aatgaaaata cattcggcgg aataaaggaa cttttttaat gatgtataag atcaccttgc
     1741 gcaggattgt tgcgcaaaat tattgctttt tgaataccaa tgaggcagaa ttgatacaat
     1801 aacgcaatcc ggttggtgtt tgcggtgcat catcaaatac gtgtccaaga tgtccatcac
     1861 agcgggcaca gtgtacttcc gtgcgcacat aaccaatatc cagatcggtg tccttaccaa
     1921 tattcaagtc gttgatcggt gtatagtaac tgggccaacc ggtgcctgag tcaaatttcg
     1981 tttctgatga gaacagtggt agtccgcaag cagcgcaata ataaatgccc ttctcatggt
     2041 tgtcgtaata ggcattttga aatggtcttt ccgtaccggc ctgacgcatt acgtaatacg
     2101 tcataccatt gagttctttc tgccacactg aatcaggctt agtgatgatt ttgccgtcaa
     2161 aaacataatc agcagactca gtcgtaacct tattatctaa agtttctata cttgagttag
     2221 tttcagtggc tttctgagta ttctggctgc atccgaaaat tgcaggcatc aatgcaataa
     2281 caacaataag tgttcgcata cataaagaat taagtattct gttcatgcca atatttacgc
     2341 attacctggg taaacagatt ggcaggtata ttagttcagt agcttgaaag gctattcagc
     2401 agtcacactt gacggataca tttgatcctg aagctctttc accttcggat ccaaaatata
     2461 ctcgtcgtag gtcatcaaac gatcgattat tccattaggc gtgatttcga taacacggtt
     2521 tgcaatcgtt tgcatgaatt ggtggtcatg cgaagtgaaa attacaaagc ctttaaaatc
     2581 agccaatgca ttattgagtg cttcaatact ttccaggtca aggtggttgg ttggttcgtc
     2641 catgatgagt gcgtttccac ccaataacat ggcgcgtgaa atcatacaac gcactttttc
     2701 gcctccggac aataccttgc agcttttaag tgattcttct ccactgaaca acatacgacc
     2761 aaggaaacca cgaatgtatg attcgtcttt ttcttttgaa tattgacgca accagtcgat
     2821 gaggttcatt tcaccggcaa agaaactgct gttatctgtt ggaaggtaat tagatgttac
     2881 tgttacaccc catttaaatt caccctgatc catttggcgt tcaccggcaa taatttcgaa
     2941 gaaataagtt tttgcaatag ggtagcgact tacgatcgca attttatcac ctttttcagc
     3001 agtaaaattg acgttactga ataggtttcg accctcaagc gatttgctta cattcgtaac
     3061 ttgcaaaacc tgatcgccaa gatcgcgttc aggagtgaaa aatatacccg gatattttct
     3121 gctcgaagcg cgaatctctt ccacattaag cttttcaagg gctttcttac gtgaagtagc
     3181 ttgttttgcc ttagaagcat tcgcagagaa tcgactaatg aattcctgta actcgcgacg
     3241 tttgtcttcc acttttttat tcttctgagc catcaattgc aacatcaatt gcgatgattg
     3301 ataccagaag ctatagttac cggtgaagat atttatctta ccataatcaa tatcggcaac
     3361 gtgcgtacaa actgcatcga ggaagtgacg gtcgtgggaa acaacgataa ctgtattttc
     3421 ataatcggca agaaagtctt ccagccatga aatagtgtca atatccaggt cgttggtagg
     3481 ctcatccatc aacaagatat ccggattacc aaacagtgcc tgagcaatca gcacgcgtac
     3541 tttctcttta ccggaaatct gttccatggg cacataatgc agatcttcct ttacaccgag
     3601 gttactcaat agctctgcag catcactttc ggcattccag ccattcattt cagcaaactc
     3661 agcctctaat tcacccacac gaataccatc ttcctcatta aaatcaggct tggcatataa
     3721 ggtgtctttc tcctgtataa tattccataa tttgcggtga cccatcatca ccgtgtgaat
     3781 tacagagtgc tggtcgaact cgttgtggtt ctgtttcaat accgccatac gctcgccggg
     3841 agtaatatca attaatccgg tggtaggctc gatttcacct gccagaattt taaggaaagt
     3901 ggatttcccg gcaccatttg caccaatgat accatagcaa ttgcctttgg tgaatttgag
     3961 gtttacgtct tcaaacaaaa ggcgtttgcc atagcgaagc gatacattat taacagtaat
     4021 cattggtcag ctcagaattt ggaggtgcaa aggtaagcat ttctcatgga ttttttatgc
     4081 gcaaaatcct tgagggatgg gggtttcagc aaaaatagat tattcggcca tcaggaggct
     4141 gatgtcttta ataagcttat tgacttcaac ggtatctgtg ccatcataat acccacgaat
     4201 acggccttcg gggtcaacaa gtgcaaagtt ctcagtgtgc acaaaatcag tagggccgcc
     4261 atctccaact gaagcgtcaa caagatatga atagcgcgca aggtcgtaga tctgcttctt
     4321 atcgccggta acgaactgcc atttggcatt attgatatct ctgtttccgg catatcgctt
     4381 cagaatgtag ggtgtatcat ggtccgggtc gacagtatga gaaagaaaca tcacattggg
     4441 tgtagtttca taggcatgat agacttttaa cattccctcg gtcatgatgg ggcaaatacc
     4501 ctgacaggtg gtaaaaaaga aatcggcaac atagattttt cctttgtagg gagtcatatc
     4561 caataataca ctatcctggt tcaacaggga gaaatcctgc acataatgtg gttctccatt
     4621 gctgcctcgt gggccataaa aaggcagttc aagctcctta ttaccgctca tagtgttatt
     4681 gtaaaccatg aaaattgcaa aaactacaag ggcagctact accagcgtgc catataggaa
     4741 gagtttattt tgtttgccgg ccatgctgca aaattacgtg tcggtcggca ggttatagtg
     4801 cgcatttacg aaaagtttaa aatgtattta accggtagtc aagccttacc cccaaggtca
     4861 aatgaattat cttagggcgc aaaacttgtg catgcagaaa tctaacaggc ttacactcta
     4921 cattttggca gccatggtgc tgggcatatt tgtcggatcc atgatccata attccgggaa
     4981 atctgatgaa caattgggag cgattgcttc taaaatcaac atcaccacaa ccgtattcct
     5041 ttcactgatt aaaatgatta ttgcgccatt ggtgctttca actttaattg ttggaattgc
     5101 taaaatgggg gatgtgaaaa ctgttggaag ggtaggaggg aaaacaatgt tgtggtttat
     5161 tacggcaact attatttcgc taacgcttgg gatgtttttg gttaatgcgc tgcaaccggg
     5221 ggaagctatg aacctggcac ttcctgcaga aactgcaagt gcgggcgttg atgcaggagc
     5281 tattacactt gaaaaattta ttacgcacgt tttcccaacc agtattttta aagcgatgtc
     5341 tgaaaatgaa atattacaaa tagtagtttt cagtattttc ttcggagtgg cttgtgctgc
     5401 aattggggaa aaagcagcgc cactcattaa agcattggat atagttggac acgtgatgat
     5461 taaaataaca acttacgtta tgctatttgc accctttgca gtgtttgcag ccatgtgttc
     5521 tgtgattaca acaaagggta catcggtgct cttaacgtac ggcatattca tgcgcgattt
     5581 ttatttcgga ttgctgatat tatgggccat tctcattatt gccggatttg cagtattacg
     5641 aaaaagagtt ttcattttat tgcgaagaat taaggagcct tttttattgg cattttcaac
     5701 agcctccagt gaagcggcat ttcctaaatt gataactgaa ctagaacgat ttggctgtaa
     5761 taataagatt gtcagtttta ccttgccctt aggatattct ttcaacctcg atggcagtat
     5821 gatgtatatg accttcgcat caatatttat tgcacaatgt tataatatcg atctcactat
     5881 gtcgcaggaa attaccatgt tgctgacatt gatggttacg agtaagggaa ttgcggccgt
     5941 tccgcgggct agtcttgtag taattgcggg aactatttca atgttcaata taccacaagc
     6001 cggattagta ttattactgg gcattgatac ctttctggat atgggtcgca gtgctacaaa
     6061 tgtgatcgga aatgcaattg ctacagctgc tgtcagtaag tgggaaaagg cattggagga
     6121 ttgaggatag caatataatt ttaaataatt aattatcgca aatatggctg tcccatatca
     6181 aataacatct gccgaggcct atcaacatgc ttatgctaaa agtattgaaa atcctgcagc
     6241 attttgggct gatatcgcat cgcattttac ctggaaaaaa caatgggaat taaccctcga
     6301 ctggaatttt gttgaacctc atgtgcgatg gtttttaggg gggcaattga atattacgga
     6361 aaattgtatt gataggcatt tggaatcgct gggaaataaa cctgctatta tttgggaacc
     6421 caatgatcct gaagagtcgc accgtgtgct tacctatagg caattacatg ataaagtttg
     6481 ccagtttgcg catgtgctga aaaataacgg agttgaaaag ggtgatcgcg tctgtattta
     6541 tatgggtatg attcctgaat tagccattgc agttttggca tgtgcaagag ttggtgccat
     6601 ccattccgta atttttggcg gattcagtgc ccaaagtatt gccgatcgtt tacaggatgc
     6661 gcaagcacat tttatcatta cttgtgatgg cgctttcaga ggggctaaag atattccctt
     6721 gaaaagcgtt attgatgatg cgctgattgc atgccctttt gttgaacgtg tgattgtttg
     6781 cacaaggaca agaactccgg taagtatgtt gaaaggcaga gatgtgtggt gggaagatga
     6841 aattaaaaaa gtggagacat taggtcttca taatttcgaa cctgaagtaa tggagagtga
     6901 agacccattg tttattttat ataccagtgg atcaacagga aagccaaaag gtgttgtgca
     6961 taccacggca ggatacatgg tttatgcaaa ttattcgttt gtaaatgttt ttcagtatca
     7021 gcccgaagaa atacatttct gtactgcaga catcggttgg ataaccggtc atagctatat
     7081 cgtttacgga cccttaagtg cgggtgctac ttctttaatg tttgaaggta ttccaacctg
     7141 gccggatgcc ggtaggtttt gggatattgt tgagaaatat aaagtcaata ttttatacac
     7201 ggcgcctacg gctatcagaa gtttgatggg tttcgggaca gcgccaatcg agggtaaaga
     7261 tttaagttcg ctgcgcgttt taggaactgt tggtgaaccc ataaatgaag aagcgtggca
     7321 atggtatcat aaggatatcg gaaaagaaaa ctgtccgatt gtggatacct ggtggcaaac
     7381 agaaacaggt ggtatattaa tttcaaatct agccggtatt acagaagcaa aaccatccta
     7441 tgcaacttat ccattgcccg gaattgaccc gattttggtt gacgaaaatg gcaatataat
     7501 tgaaggtaac aatgtcagcg gaaatttatg cattcgtcac ccctggcccg gaatgttgcg
     7561 cacaacgtat ggcgatcacg agcgatgccg acaaaattat tttgcaacgt atcatcaatt
     7621 atattttacg ggagatggcg ccttgcgtga tgaagccgga cgataccgta ttacgggtcg
     7681 tgtagatgat gttttaaatg taagtggtca ccgaattggg actgctgaag tagaaaatgc
     7741 tataaacatg catgccggtg ttgtagaaag tgccgttgtt ggatatccgc atgatataaa
     7801 aggacaaggg atttatgctt ttgtagtgta cacaggtggt catcaatctg atccgcaatt
     7861 aacgcgacaa gatattttac ttacggttac cagagcaatt ggtgccttgg caaagccaga
     7921 taaaattcaa ttcgtatccg gattaccaaa aacgcgaagc gggaaaatta tgcgtagaat
     7981 tttgcgcaaa atcgctgaag gagaacccgg gcaaattggc gataccacaa cattgctgga
     8041 tcccaatatt gtagaagaga tcaaatccgg ggtaattaat taattatctt ctccctttat
     8101 acgggaatga taataaggat ggatgtttat cttgtccttc gttggtgatg atcatagtgt
     8161 tatcagatga aaatgcaatt ccttcaggtt tgttaaatac gttttcatcc agtgaaatca
     8221 aactaactaa tgatccattg ggatttacta tacagagtaa atgatcaact gccgacaata
     8281 aataaatgtt gtggttctca gggtggatgg caattgctga tggtctaaat ttaatatccg
     8341 gttgttcttg tttctttttg tgctttgact ttttctccgg aagatccatt gcatgcgttt
     8401 tagcgtaatc ctgtaattct gaaatgtaaa cttcaaaaac tgggtcactg ctaagttttt
     8461 tagtgccaag gtcaaatgca taaatagcgc gcacattttt taatgttgca cctttaccaa
     8521 tattactttt gcatgcaatc agcaatctgt tgtgttccgg atcataacat aatccttcat
     8581 tgttattcgc agggatgcct gttttgaagg taatatgccc atattcaggt gaatggaaat
     8641 caggaatttc gaataataat ccatcgctcc gcaatataaa taaactgtta tttacccttg
     8701 caattccttc ataatcccca tcgccgaaaa atggcatttg tttgataata tttcctgtag
     8761 ttacatcaat tatgaatact atgccttgtt cgtcctgaat acaggcaaag gtgtccatgc
     8821 ttatttgggt aattcctgaa atttcctgca acgaaggggg tagtaatttt ctgaatcccg
     8881 gttcttccag gttatatcct acggttctgt attcttttgg aagtaataca gcacaactat
     8941 tcaaaagcag taatgttgct gcactgaaaa cccacagtgc ccggttaact gttagtccaa
     9001 gttgatattt tatgttgtgc ggagtcgtaa ccatattcct tatctacaaa tctataactt
     9061 tgcgcctcaa catttgaaaa tgtcagagaa aaggcaggcg tccatgattt ttatattcat
     9121 caccatgttg attgatgtgg tgggttttgg cattattatt cctgtattgc cgaatctaat
     9181 caaagagctg cacggaggtg attacagcga agctgcgagg ataggagggt ggctgctatt
     9241 tgcattctca ttaatgcagt ttttatttag tcccgttctt ggtaacctga gtaatcaggt
     9301 tggtcgtcgc cctgtactgt tattttccct ttttggcttc ggaatagatt acctgatcat
     9361 ggcatttgca ccaagtttgt ggtggctggt agttggtagg gtattggcgg gaataactgg
     9421 tgccagtttt tctacggcga gtgcttatat agcagatatc tcaacaccgg aaaaacgggc
     9481 gcaaaatttt ggacttgtcg gcgctgcttt tgggatggga ttcataattg gtcctgccct
     9541 aggtggattg cttgcgggtt taggtcccag agtgcctttt tatggtgctg ccatattatc
     9601 gttggtgaac tggttgtatg gctttttcat tttaccggag tctctgccaa aagaaaagcg
     9661 tagaaaattt gaatggaaaa gagcaaaccc aatcggatca ttggctgcat taaaaaagta
     9721 tccgttgaca tacggactga ttgcaagtat gatttttatt tggttggcat cgcatgcggt
     9781 tcagagtaac tggagttatt tcacaatggg aacgtttcat tgggatgaaa aagcggttgg
     9841 attttctctg gctttggttg gcgttttggt aggtcttgtt caaggtctcc tcatcagagt
     9901 aactatacct cgatttggcc agaaaaattc cttgtattat ggtttattac tctacaccat
     9961 cgggctatta ttatttgcat ttgcaacaca aggctggatg atgtatgctt ttctggtgcc
    10021 ttattgcctt ggtggaatcg gaatgccggc tttacagggt atcatgtcgt cacaggtgcc
    10081 tgcaaatgaa caaggtgaac ttcagggagc gatgacaagt ctgatgagtg taaccagtat
    10141 cataggtccg ccattgatga ccaatactta cgcctatttc actgctaagg atacgcctta
    10201 cttttttcct ggagcgtcgt ttttacttgg tgcaattttt atggcagtga gtttattgct
    10261 cactatgcgt acgcttaaaa agcattgatc attcaaagta ttcgtgatac ttggacagat
    10321 ggtctttaat tttaggaagc tgagccttgc aagtttctgt accacttacc tgcaatggaa
    10381 cgataagttc ctgtaaggga tttaaataag catgcaccat ttcgtggttg ttgcccattt
    10441 gttcacagtg gtttacgata taaatcaggt cttcaactag ttcgctgcct agagcctggt
    10501 aagccgcact atctgcatca tcaggaaact tgttcagttg aacaatcatg gaatcaatac
    10561 cctccgtagt ttcaatattc gatttccacc gtttgccatt gtctaaatat aaggctgaac
    10621 ccctgcaaag gatatactta ttctccggag cctgcagata aacagcatca tcctgacggc
    10681 aagctatcaa tgtgagtcct aaagcaaaga atcctaatat aacaggaggt agccgcatgg
    10741 cggtataaaa ttagatgcct ttttaacttt caggcaatga tttaaagccc tttgcaattt
    10801 ttttacagtc cttttgatga acaattgatt aaacatgacg ttatactact aatttactcg
    10861 aataatgaac gacaactcac tactctggct tggctttaat gcttttgtat tgctcatgtt
    10921 ggcactcgat ttgggtgttt ttcacagaaa atcacatgta gtcggcatca aagaggcact
    10981 cgcgtggtcg ggcgtgtgga ttgccctcgc attggtattt aatggcctaa tttattttgg
    11041 aatagttggt ggttatgaag gtgctgaatt acagaagcag aaagggatgg aattccttac
    11101 cggttatctc attgaaaagt cgctgagtat agacaatatt ttcgtcttcc tgttgatttt
    11161 taccttcttt aaggtgccac aattatatca acataaagtc ctgttttggg gaattattgg
    11221 tgccttggtg atgcgtgcaa ttttcatttt cgcaggtgtt gctttgatag agaaattcca
    11281 ctggataatt tatgtgttcg gcgtcttcct gatcatcacc ggtattaaca tggtgcgcaa
    11341 caaaaacaaa caattagatc cggagaaaag tcttgtcatt aaagggttta ggaaaatatt
    11401 tccggtgcac aacagttacg acgggagtaa attttttgtg acacttaatg gtaaaaggca
    11461 cgctacttta ttatttgtag ttttggtttt tatcgaattc accgatttga tttttgcagt
    11521 cgacagtatt cccgccattc ttgccattac gccggatcct tttatagtat atacttcgaa
    11581 tgtatttgca attctagggt tgcgcagttt atattttgct cttgcaggga tcatgcatcg
    11641 atttgcatat ctggtgtatg gattagccgc tgttcttgtt tttgttggtt tgaaaatggt
    11701 tttcgtagat gtttataaaa tacccgtgca atattctctg cttgtaatag ccatattact
    11761 tgcagcgtca attttctatt ctatatataa aacaaggaaa acgaaagcct gaattactga
    11821 tgctattcca catcagattt gatcccacgc accttgagaa acccaattgc aattattgca
    11881 cagaaaatac caaaggtcca ccataatgca tcgaatccaa gtttgtcgat gatctgtgtg
    11941 ctgcaatagg gtgaaacgat aaacgcaacc gaccatgcga tggtataggc tgccaaatgt
    12001 gagccacgtg tttgtggggt tgccgttttc actacatgtg atatcataaa tggcatggca
    12061 aatatttctg aaaaactcag taaggtcatt gcagtgatta atacccacat gccggtgaat
    12121 aaattgaaaa tggcaaatga aacgccaagc atcaaggttc ctgcaataat gagccgctca
    12181 ggtttaaatc gcttttctat ttgcgatacc gtgatcattt caaataaaaa tacaatcacg
    12241 ccattaaatc caattaataa gccgatagct ttttctgatt gaaaatatac gtctttgtaa
    12301 tataagggta tgctgctaaa aatctgaaaa aacgcggctg cataacaaaa acataaaaat
    12361 agaaaataca aaaatgcagg ttctgtatac ggcgatttct gtggttgatc ttcctgttta
    12421 tcttctttat gaatttgtgg ccttttacct ctgaaataaa agaaaaacac aactcctgca
    12481 gcaatacaag taataccatc accaaaaaac aaccagaaat aatttgtttc tgccaataat
    12541 ccggcaactg caggaccaat agccacaccc agattgattg ccattcgcat taatgaaaac
    12601 gaacgagttg ttgtttcctt ggtagcaaaa tgtccaatca tcgatgacgc tgcagggcgc
    12661 atggcatcat tcacagttgc acaaacaaaa atccctaaag ccagcgaata aaatccggtc
    12721 agcaatggca ataataaata acacccaccc ccaaataaca aactgaataa ctgcacttga
    12781 aatggcccga atttatctgt ccacctgcca cctaaatacg atccaagcaa tccgcctaaa
    12841 ccatataccg acatgattat tcctgcctgc acagtgctgt atcctaaatt ggcagtcata
    12901 taaactgtca taaaaggcaa aaccatgctg ccactgcggt ttatcaattg aatggcagcc
    12961 agtatccaaa cagatctgtt tattccccta taggaatcaa gataaatatt gagcgccttg
    13021 ccaaacacag caacgcgaag gtattataat taagaatgaa gaaaatctga ttggcgttga
    13081 taatcaacta cgaagggtca ttccatcgat acatcgggac tgaaaagtct gctaattgaa
    13141 gtatcctgtc aacaacagta gcagctacct cttcctgaga tttcggtttg ctgtaaaatg
    13201 agggcgacaa tgggcagatg ataccgccgg ctgtagtaac cgtttccata tttctgatgt
    13261 ggatcaggtt gtaaggggtt tcacgcaccg ctaaaataag tttcctgcgc tcctttaaca
    13321 tcacatcggc agtgcgggtg agcaaatcat tggaaatccc ggatgcaatt ctgccaagtg
    13381 ttcccatgga acagggacaa acaatcaacg tatcaaacca ggctgagcca ctggcaaaag
    13441 gtgccatgaa gtcgtttttg ccataaaaag tgaaagggta attcgtaaat tgctcattat
    13501 caagctcaaa gcgccaaaca tccttagcat tatcgctcat aactacgcca actttctcta
    13561 tttcagcacc taaaagctgt agcttgtcca gcaaaacttt ggcataaatg gcaccacttg
    13621 ccccggtaat tcctactgct atcttccttt ttctcatctt tgtccgcgca attccattgc
    13681 aagttaacca ctttggaatt atttttgttt acaggtagtt gaacgaatca atactatgca
    13741 aaagagaata ctgatttctg cagccttaat tatgctgctt ggtgccgcac tgatggcttt
    13801 tgtgccgatg gacacactta aggaagcgtc aggtgcagat ttaatgatgg ctaccaccgt
    13861 tgaagattct gccaaaactg aattcaaatg gtacacgatt tccgaagcat tggaaatgca
    13921 gaaaacatcg cacaaaaaga tttttatgga tgtttacact acctggtgtg gaccatgccg
    13981 catgttagac gcaaatacct tttctaaccc cgaaatccaa aaactgttgt cggaatacta
    14041 tattcccgta aaattcaatg ctgaaagcgg ggatacgatt caataccagg gtcaaacctt
    14101 cacaaatcgc aactatagtc cactaccgcg caagagtact catgattttg cagtgtatat
    14161 cgcttctaca gagcagggat taggttatcc taccgtagta ttcatggatg aaacgggtac
    14221 tcgtcttcaa ccaataagcg gttatcgcga agcaggtaag ggaacagctc aaaatccggg
    14281 aatggaacca attttatcgt tttttggcac agatgcttac aaaacggcca cctggaacga
    14341 tttttatgct gcatttcagt ctaatattaa gtaatccatg tttattgaaa tagcactttc
    14401 gcttgtgatg ttcttccatg aggatcatca agaaactatt gctcctgctg cagaacaggc
    14461 tggtgttgat accattggat ggatgtcact ttccgatgtc aacaaaattg ttgaaaagaa
    14521 catcaaaaag ggtaaggtaa aggaggataa actggtaata gtggatttct acacagactg
    14581 gtgcggctgg tgcaagaaac ttgacgctga aacgtataag gatccggaag tcgcagcttt
    14641 gatgaataaa tacttttatc cggtaaaatt tgatgccgaa tctaaagata ccgttgcttt
    14701 tgccaataag acttatggtt ttaaaagctc cggcagccga ggcactaatg aattcgcctt
    14761 ggaaatggca agcaggccgg gcggtagaat agggtatcca acaattacca tcatcaatgc
    14821 tttgggagaa aagctgaccg tagaagcagg ctataaaaat cccgaaaaaa tgaaattgat
    14881 gctgctgtat tacggtgaag gacactataa aacgaaggac ttcaacactt ttgtcaccga
    14941 atacaatcaa aaacaactaa ctgattaaga ggttctgcag aaatagtgca taaacaggtg
    15001 cataatattt acgggattcc tttcacgata taatggctag ggttaacttt gcccggttta
    15061 caatatgtcc tcagcagcaa aaaatatcca taagctatca gctgccggcg tactgatctc
    15121 cctgggaatc atttttggcg atatcggaac ttcgccaatc tatgtgatca gtgctattgt
    15181 cggcgatcat gtcatttcgc gcgagttaat ccttggcggc ttgagttgtg tattctggac
    15241 actcgtcatc atcaccacat tcaaatacgt cattcttgcc ctgaacaacg ataatagggg
    15301 agaaggaggg atatttgccc tttatgccat tcatcgaagg tttaaggtta aatgggctat
    15361 tgttcctgca ttggttggat gtgctgcctt gattgctgac ggatttatta cacccccaat
    15421 ttctatctcc tctgcaattg aaggtctaaa actgctaaac ccgaacatcg actcagttgt
    15481 gccgattgtg atcatagcaa tgatcatatt atttaccgtt cagcaattcg gtactaatgt
    15541 ggttggaaaa gctttcggtc cggtgatgtc gatttggttt ttaatgatcg gtattctggg
    15601 ttctattcaa gttgtgcaac atcccgaagt gcttgaggct ctaaatccat actgggcaat
    15661 ccatttactc acagaatata aaggaggctt ttggctactc ggtagtgtct ttctctgtac
    15721 tacaggtgcc gaggctatgt atagcgattt gggacattgt ggtaaacaaa atatccgatt
    15781 ctcatggaca tttgtgctta ccatgctgtt gttgaattat gccggacagt cggcttacat
    15841 gttaaccatg gatggtcagc aactcaacga ccatagtccg ttttatatga ttatgccaac
    15901 atggttcctt cccatcggaa ttattgttgc taccagtgct accatgattg ccagtcaggc
    15961 aattctcaca ggttgtttta cgctggtgaa tgaagccatg aaattaaaac tgtggaccaa
    16021 ttttaaagtg gtataccctt caaccgttaa aggtcagatt tatattcctg ccatcaactg
    16081 gtttttgttt gccggttgcg ttaccgtagt actgttattt cgcgaggcaa aacatatgga
    16141 agcagcctac ggtttggcca tcacttttga tatgatcatg accacggttt tgcttaccag
    16201 cttaatgtta ttacacaagc gagcaaaact catggtgttc agcctcgcag cattgttttt
    16261 gttgatagaa gggtcattct tagtgtcaaa tctcaataag tttacgcatg gcggttggtt
    16321 taccgtgtcg cttgcagttg gtattttctt tgctctgtgg attttctacg aggcaaggaa
    16381 attacgcgcc aagcataccg aatttgttga gatcgataaa tatctccctg ctattcagga
    16441 tttgatgcac gatgaatcca ttgctaaaga agcaacgcat ctggtttacc tgagtatggt
    16501 gaacgataag aatcatatcg atagtaatat catctattcc atattccgaa agaaacctaa
    16561 gcgtgccgat gtatattggt ttgtgcacgt agatatttca aatgatcctt atctggcaac
    16621 gtattcggta gataccattg tgccgaagaa gatcttcttt gtgcgattgc ggttcggatt
    16681 taaagtagaa cacaaagtga atgtcatgtt ccgtcgcgtg gtggaagaaa tggttgctgc
    16741 aggtgaagta gacttgctga gcaattatcc tagcctccgc aaacatggca tgtatgccga
    16801 tttcaaattc atcctgctca actcacgcgt ttccattgat aataaattat caccatggga
    16861 acagatcgta attaaaggat acagattctt caaatcgctg tcgttgccaa cacacgagga
    16921 ttttggtctg gaactcacca acgtagaata cgagatggtt ccgattaaag taagtcagga
    16981 aatcgagatt aaactcgaac gcgttacaaa gtagattctt cgctggctac gggtttctca
    17041 atacgatcaa cattcttttg gaatatcgga cgcatggtgt caagatctaa ggcacaaagg
    17101 tgtccatatc ccgggcgacc aacataaacg catccgcaat cgatgtttac ggcaggatat
    17161 ttcatttcac tcaactcctg ttcaagcgtg ttgaatggtc tgctggtatg tccatgcaca
    17221 atgatcttat tattaatttt ttcagggatg atggtatcgt agtaattgcg aatccaataa
    17281 agactgtgag tatccttgaa aatatcttct tccttaaagt taaatcctgc gtgaaccaaa
    17341 atcaccttgt ctaattcaat ataaggcttc aagctgcgca cccaggtatc atatttctta
    17401 tcaatgtttt tcagatcatc aatacgtttg taacctaaat tcatcatggt tgttacaccg
    17461 ccatttttca cccaagcctg atagcgacgc cagtcccagc aatatggaat agtttcatcc
    17521 aacgcttgca taaacatcca ttcgtgattc cccatcaagg ttgtcacttt gtagccttct
    17581 tcaaccattt taagcacctg atcaatgact gcaatgctat ttggtccgcg atcaatcaaa
    17641 tcgccgagga tgtataattc gtcctctttg gtcaattgta aggtgtcaag aagggctgaa
    17701 aatgtctggt gacagccatg aatatcggaa attgcaaatc tgcgcatagg tctaattcag
    17761 ttttaaattg ttgagacaca gcaccttaag tttcgaaaca tcggggattg tgccttcaga
    17821 aatatcctgc aaagatattt gttttggggt attgggcaac aggtcgacga agttattgct
    17881 cagtctgagt tctgtatcat cgctgtagag gtaaacttgt cgggcaaata ctggacttgt
    17941 caatgtaata acatcaccat cgatactgat agctatatct gcttttttgt agctgacagc
    18001 acccggcctt acaaaggtgt gcattcgttc cgcaatcacc ttgtcttcct cactcagcga
    18061 tagccaaaga atacaatttt cctttgcata tccgctcaaa aaccattcaa ctcctaatgt
    18121 tgaatataac atggaagtat ttgccttaag tgtaaccggt aaagtctgat tgcgaatggt
    18181 aacgcctgat gttgttaata atgaataggt caattgcaat tttctgtcag tagtatcatt
    18241 atttaccaac catgtttgca attcatctcc ttcctgtaaa acactgatta tcacatcgcg
    18301 atagcatgac tcaacagtat accaggcagc ttttctattc ccataaaaat cttgcacact
    18361 ccagcttgtt acaggccaac aatcattcaa ctgccaaaac atcgttccca tgcaataagg
    18421 catatttcga cgatgcgctt caatcgctac acgcatgcca tcagcttgca acaattgcga
    18481 aatgtaaatg taggattgca ggtcagatgg cgtgccataa tcgcgcttca tatattcatc
    18541 gatggtttca taaccgacgg gatgtttttg atgcgcttta aaggccggag agccaagata
    18601 taaattgtct gaattaatac atttttccca actatgatat gagggtatgc cttgaaatcc
    18661 atattcactc ataaatcggg gcaccttttt attatagatg tcaaatggtt gtttccccca
    18721 ccaaacgccc cagtagtgtg catctccgga agtcagactt tcttttcgtc cccagccgta
    18781 agtaggtgag gagggatgat aactttggta cgcaccggca tttgaatgta aatactcagg
    18841 aatcactaat cgaaataact tctcgttcaa cgaagcaaca taggccgaat catcatcgtt
    18901 gtatttcaat tgtttttgcc atccccagtt attccatcct tcatcaattt cattattgcc
    18961 acaccataat gctaaacagg ggtgattttt gaggcgcgtt atctgttgtt gaaattcggg
    19021 atattccgtt acgtcattcg gatacatggc gcaagcaaac ataaaatcct gccataccat
    19081 tattcccaat gaatcgcaaa gatcataaaa aatatcctgc tcataaatgc cacctcccca
    19141 aacgcggatc atattgatat gcatgcgctt tgcttctatt aaaatatcgc gataacgctc
    19201 atctgatgct tctgttggga aactatgtgc aggaataaaa ttcgcgccat ttatgaagac
    19261 ctttttacca ttcgcagtaa aataaaatga cttaccactc gcatccgctt cctgatgtaa
    19321 ttgaatgtta ctaaatcccg ttttgaatac ctgctgataa taaatgcgtt tgttatgtgt
    19381 gatttttaat tcaaatgcat aaatattttg agatcctaat gcatagggca tccaaacttc
    19441 aggagcatgg aaattgaaat tgatttgttc aaccgtaggc aattgatctc tataactcca
    19501 gctatagcag gttttgcctg tagtgagttc tttcacttca acctcgtaat gcatacgacc
    19561 taaagcttca tatgccagat caatatgcac agggaactgg taatcaggat tttgttcggt
    19621 ataaaaatca acaaacttga ttttcaattt atcattaaaa acaagtgtta taggctgcca
    19681 aatgcctgaa gttaccaaac gcggtcccca gtcccaaccg aattgatatt gtgccttgcg
    19741 aatatacaca cgttcatcgc tgggcaatgg cactgtaaat tgctcaccaa cttcgtttgc
    19801 tcgttttacc ggcgaataga aatgaatgaa taacagatta tcgccacttt tcaaaagagt
    19861 cttcaccggc actttatatg ctcgaaacat atttgttgat gacaatatca gtgagtcatt
    19921 caaatacaca tctgcataag tgtcaagtcc gccgaaattg agatcaataa aatcactgtt
    19981 taattgattt ttatgtagat gaaatgtagt tcggtattcc caatcttcat tctctaccca
    20041 ttgcacagct gcttcatttg tgtcgatgaa aggatcaggg atggcattat ttgttagaag
    20101 atcagtatgc acacatccgg gcactttggc tttcatccat tgtgtatcgc caaagcgacg
    20161 aaattgccac tgttgatcaa gtgttatact ttccatttgc gcatgaacag aaaagtgtag
    20221 catagaacat gccaatatca aaaggtgtat actcttcatg agttatatgt agcaataatg
    20281 gcacttaatt tgtgtacctc ttccggattt acttcggtaa gatgtgaggt gtcgtaaatg
    20341 tttgaattaa agttatagga agttgacttg ggcgtgaatg gtcgctttgc agaagtgtgg
    20401 atttcgttgc aaccggtata gtcaataatt tcacgtgcat tactgctatt aacgcctgct
    20461 cccggaagta aagtaatgtt attgtttgcc gatgtaatca attgcttgat tatttccttg
    20521 ccttcaattg cggtgtttgc ctgacctgaa gttagtattc ttgtaaatcc acaggtaatg
    20581 gcatcttcta atgcttcaaa gggatcgcgt gtaaggtcaa aggctcgttg caatgtgcat
    20641 tgcatacctg acgcagtatc aataagccgc gaatttcttt ctacatccaa ctttccatca
    20701 ggtgtggtga tgccaaatat cacttgttta acgccaaggg agcgacaatg aagtatatca
    20761 tgacgcatca catcaaattc aaaatcgtcg tacatgaaat cgccacctcg ggggcgtatc
    20821 atgacgacaa tattttccgg atctatcatt tgcatggcag cagtgatagc tgcagaagaa
    20881 ggagtgatgc cgccttcaac ataacttgca cataattcaa ttctatcggc gcctgcagca
    20941 actgcatgca caacatcagt ggcattgaat gtgcatattt ccagtatata ctttcttgtg
    21001 actgatgttt tcatttattg ctcagggtaa tagctgtctg atttgaatag ttcgttggca
    21061 atattattcg aaaacacagc ataattaaat ccgggttgca ggcaataagc gccaaattca
    21121 ccagctttgt tcaatgcgat gaaaccgacc tgaaaatctt tagttttttc tttgcggatc
    21181 tttaccatac gcatcacggc ttctttgcat gcttcgtatg gcgagtattt ctgccgcatt
    21241 aattccacta ccagatgcgt acctgcaatt ctgatcactt cttctcccgt tccggttgct
    21301 gttgcagcgc cgatttcatt atctacatac aatccggcac caatgatagg tgaatcaccc
    21361 acacgaccac gcactttata agcaagaccg ctggtagtac acgcaccgga taaattacct
    21421 tgcatatcca tggctatcat cccaatggta tcgtgatttt caatatttat gacaggttta
    21481 tattgttttt caacaagcca tgcttcatat gctttttgtg cttccggatt tttatagtcg
    21541 tcaagtgtga atccattttc cagtgcaaat tgttgtgctc ccgaaccaac aagcataata
    21601 tgtggtgttt tttccattac catgcgcgca acagaaatcg gattttttat tttctctaaa
    21661 aacgccacgc ttccgcaatt gtagtgctca tccatgatac aggcatccag cgtagtatgt
    21721 ccttctcggt caggtaatcc tgcaagtcct actgaaagag aatgcaaatc attttcagca
    21781 actttcacac cggcttcaac cgcgtcaagt gcgcgacctt tggcactaag tatagcccat
    21841 gcatcaacat ttgctttaag accaaaatcc catgtggata ataccaaggg tttattcatg
    21901 ggaactgact tgtgctgatt ttcaactacg gcagattgca gtgactggcc ggtgctgatg
    21961 acgcctgctc caagggctgc agtttttata aacgatcttc tggatgtcat gcaacaaata
    22021 tcggaaatgc atttgatagc aggaagacgg cgtcttttgc aaattgcttt cagactacat
    22081 gaatggtgtc atctgatatg aacctcatcg acaaacaacc atgctttgtt tccggccccg
    22141 gggtttcctt gaggaattgt gccgatatta tttgcaatta tcctgacata tcttgcggca
    22201 gtaggatcga acttataaat accattcgtt attgcacgat catcctttac cacaggcaca
    22261 attccgatcc gagtgaattt ttttccatca aggctcaccg atatttccat ggaagacgga
    22321 agatatatcc aactggtttt gtcctccaaa aacccggcac ttactgccat gatggtatcc
    22381 acagctccta gatcgatagt gcaatttaaa ttcgttccgc tgaatcccag ccattcattg
    22441 ccactccatg atggatataa tgatgcatta attccgtcaa caagtgaaaa tgctccactt
    22501 ccggagtatt gttgtgcagg tggtgttttg agagttattt tttttcctgt tgccttgttg
    22561 aacaataaac gcaattttac ttcaggatgt ttatagtctt caggaataat aagagaatac
    22621 gattcagtta caataaatgg tgctgtataa attcctttta taaaagctcc tccaaaaact
    22681 gcaggagaat ttttagaagt gtcctcatat tggtattcga atcctgttgt gttatcccag
    22741 gttcgtaaag ctatacgaac cccgttaaaa tcatcggtag gtaatacttc gggaatgata
    22801 ccgaatgcac ttttgctata atggcaatct actcgtccta gcagctcaaa ttgagtggat
    22861 aatcggcgca taaaatcagc ataattgcgg gccgtatcag gtgtccataa tacctcacta
    22921 agagctaaca tgcgtggtat taccatatat tcaacctgat cgaaattcgg aatatattca
    22981 gtccataaat ttccctgtgc acctagaata tattttgatt cctcaggcgt aagtgcatcg
    23041 ggaatgggat gatattcata taccttttgc aaaggaatat atccacctat tgcaagtggt
    23101 tcctgatagg gtagtccctg gtaataatcg aaatagcaat gcgatccggg agtcattact
    23161 gcatagtgcc ctgcacgtgc ggcatcaatt cctccattta cacctcgcca actcataatg
    23221 gcagcttgtt gcggaagatc attcgtaaga atttcatccc agcctataat attgcgccct
    23281 ttgctttgca catactcggc aattgttttt gtaaaatatc cttgcaatcc atgttcgtca
    23341 actagatttt tttctttaat caattgctga caataatcgc aatgcatcca ttgatctttc
    23401 tctacttcat cgcctccaat atgtatatat tgagaaggaa ataatgccat cacttcgtct
    23461 aacacacgat ataaaaattc aaacgtttcc ggtttcggac aaaatgcttc tttatacact
    23521 ccccatgcct gacctactga atatgttgta tctgcaatgc aacctaattc aggataagct
    23581 gctaaggctg cagcacagtg tccgggcatt tctatttcag gtatgatggt aatatttctg
    23641 tttttcgcgt acttcacaac ctcagaaatt tctttctgtg tataataacc accataatta
    23701 atcgtgtcgt attgttgatc gcgataatga cctttcatgg tgccggtgcg ataagcgcct
    23761 acagaagtta atttaggata ttgtttgatc tcaatacgcc aaccctgatc atcggtaaga
    23821 tgccagtgaa aggtgttcat tttgtataat gcgagataat cgatgtacat tttaacttca
    23881 tccaccgtaa agaaatgtct gctgcaatcc aggtgcatgc cgcgatattg gaattgcgga
    23941 aaatctgaaa tgcctgcgca tggaacctga aactgattgg tgaattttga ttcagtcatt
    24001 tgaagtaatg acatcaaccc ataaaaaact ccgcctttgt tttttccgat gatcgtgatt
    24061 ttttcttcgg tgatgagcat ggtataacca ccatccggaa tcattaaagt actgtcgtag
    24121 gatacttcaa tataatgtga aggataaatt tcttcgcgaa ctgtttttaa tggcagtgaa
    24181 tgaaatttaa tactcatgtc acggaacgta acagcatcca taaaagatgc catgtcgttc
    24241 accaaaatat aactggtagg cttaaaataa aaatatccgg ggttggcggt gtactcaaca
    24301 ggtttaggca caatggggca ttgctgtgct ttcaacatgt ttacatgaag tgtcagaagc
    24361 aaggtaaaaa tcagtgtggt tttcattgct tctaattaaa cctgcataac gccaacggaa
    24421 aaatcttttt cgataggtgc attatttgcc atttcaattc ccatgctcac ccattttctg
    24481 gtatcaagcg ggtcaataat gccgtcgatc cataatctgg ctgccgcata atatggatcc
    24541 atggtttcat tataatgatt taatattttc tcgagaagtg ctttttcttt ttcagctgta
    24601 atctcttcgc ctttagcttt cagcgatgca acctgaattt gcaataacac ttttcccgcc
    24661 tgagcaccgc ccattacggc aatttttgca ttaggccacg caacgattaa gcgcggatca
    24721 tatgcctttc cacacatcgc ataatttcct gctccaaaac tattgcctat gattactgtg
    24781 aatttcggga cgacagaatt tgcaactgca tttacaagct tagcgccatc tttaataatt
    24841 ccggcatgtt cactcttact gcctaccata aatcctgtca cgtcctgtaa aaataaaagt
    24901 ggaatttttt tctgattgca attcatgata aaccgtgcag ccttatcagc actatcatta
    24961 taaatcacac ccccaatctg tatttctcct tttttagttt tcaccacctt gcgctgattg
    25021 gctactatgc caacactcca gccatctatc ctcgctgtgc cgcaaacaat ggtcttgcca
    25081 taagcggctt tatattcgtc gaatacagaa ttatctacta gacgtttaat gatatctaat
    25141 acatcatatt gcttggtgat atcctcaggc atgatgccat atatatcttc ttcttttaag
    25201 gatggtgcag atggtgcaat gcgatcaaat cctgcttttg gcatagcgcc gatcttatcc
    25261 atcgtgctgc gaatatgtgt gatgcaatct tcatcatttt ctgcagcata atcgcaaata
    25321 ccggaaattt ccgtggttgt aattgcacct cccagcgttt cagcatcaat atcttcacca
    25381 attgctgctt tcactaaata aggtcctgca agaaaaatgc tgccggtgtt ttttacaatg
    25441 tatgcttcat cactcattat aggcaaatag gccccgcctg caacacagct acccataatt
    25501 gctgcaattt gtgtaatgcc cattgaactc atcacggcat tgtttctgaa aatacgaccg
    25561 aaattttcct tgtccggaaa aatctcatct tgcataggta gatatacacc ggcactgtcg
    25621 accaaataaa tgattggcaa tttattttct atagaaatct cttgagcacg taaattcttt
    25681 tttccggtta tcggaaacca tgcacctgct tttacagtgg catcattagc aacaacaata
    25741 cactgcctgc ctgaaacata acctatcatt actacaactc cgccgctggg acatccaccg
    25801 tgttcggtgt acatgcccat tgccgtaaaa gcaccaattt caatacatgg tgtatccatg
    25861 tcgaaaagta atttaatacg ttcgcgtgca tgcaactttc ctttttcatg caatttgtca
    25921 atggattttt ttccaccacc ttcctgcact ttcgtaagtc tttgcttcat ggtagatacc
    25981 aataatcgca atgcatcttc gtttctgtta aatgctatat tctgagtttc tttcgtcatg
    26041 tgtcagttct gtatgctgca aaaatacaat taccgcggca accgttgtaa gatgtggttt
    26101 tttaactacc tttgcgccgg gcaagtccta tacgaccagc tcctgctgaa tcccccaggc
    26161 cctgaccgga gcaagggtag gcggttgtag cggtgcgatg tagatcactt gcccattttt
    26221 tttataccta agcctcatgt tgaaaaatca ggcttgcggt gaacatcggg tcacctattt
    26281 ttgcaaaaac acaacttcta ctgatgaaat tttttatcga tacggcagac cttaatgaaa
    26341 ttaaagaggc tgcatctttg ggtattcttg atggcgtaac caccaatcct tccttaatgg
    26401 cgaaaatcgg cattaccggt gctgaaaata ttaaacaaca ttatatcgac atctgcaata
    26461 ttgttgatgg cgacattagc gctgaggtta tctcgactga ttttgaaggc atggtgcgcg
    26521 aagctaaaga actggttgcg ctccacccga atattgttgt taagatccca atgatcaaag
    26581 acggtgtcaa agccctgaaa tggtgtaccg ataataatat cagaacaaat tgcaccctcg
    26641 ttttctccgc cggtcaggcc atactcgctg caaaagcggg tgctacttac ctttcgccat
    26701 ttattggtcg tttggatgat atcaattggg atggcgttca attaattgag caaatagtac
    26761 atatctaccg caattacggc tatgaaacac aggttttagc cgcttctatc cgcaatccgt
    26821 tgcatattat tcgctgcgct gaggttgggg ctgatgtggt tacctgtccg cttagttcaa
    26881 taatgggatt gttcaaacat ccacttaccg atataggcct ggcgcagttc ctggccgatt
    26941 acaagaaggg taacggttga tcatatcgaa aaatctacta aagtattaga cttttaggcc
    27001 tgtctgacta tatttgcaag gcaggaatga tgtattgcct ctgttgacac agggttagct
    27061 tatccgttat caacagcaca tccatttcgg cgttaaacca tctaatacta tgcatcgact
    27121 cccatcggac gaggtcttgc catttttatt aatggtagcc actttgttgc ttgctgcacg
    27181 gctcatgggc gagttgtttc gcaagttgaa actacctcag gtaatggggg agttggttgc
    27241 cggtatcttg ctgggacctt catgcctgga acgttgcgca cccggttttt atgaatcttt
    27301 ctttgcctca cctgaaaatg caggtgttgc ttatgatgga cttgcccgta ttggcatttt
    27361 actgctgttg tttgtggccg gaatggaaat aaacctgaaa accattcaaa aaaaggccaa
    27421 tgccgctgct gcaatcagcc tttcaggggt ggcttttccg tttttaattg gatttgtagc
    27481 ctcatggtat ctgtcaagtt accttttccc ccaaggaatg ggcgataaac tggcttcctc
    27541 actgttcatg ggaacagcac tgtccataac ggctctttct gtgctggcta aaatacttat
    27601 cgatatagat aaga
//
LOCUS       DAOQHY010000017        26952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_780_length_26952_cov_4.969067, whole genome shotgun sequence.
ACCESSION   DAOQHY010000017 DAOQHY010000000
VERSION     DAOQHY010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 26952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26952
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_780_length_26952_cov_4.969067"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..3308
                     /locus_tag="PLL28_02645"
     CDS             54..3308
                     /locus_tag="PLL28_02645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68241.1"
                     /translation="MIKNGPSSSVVVIGASENSPGSWDFAAVKYSQSTGSVVATNRNS
                     ASGSGFDQVFGADVDASGNFYITGRAAVVDEGFNMRTVKIDPSMDLVWAKNYDHAGLD
                     DESHGVVVDLDDNVYVTGWITNEDGTRSFETIKYNSSGTPQWHQEVNAENVGLDAYSL
                     KISSAVDGNIIVAGNVDNGASLDYLTVIYNEDGDQLWMEQYDSPDGDDDKVNFVKADI
                     GNAVYIGGKSYSISTATNRLVKYNWEDYIVPPDDDLDHPSQFTFFENRGQIIDTDENP
                     VNDIKYYTFHQTPGLFVFDDKISYVWGRIDTTTNTDDTLSRIDLSFPDCRVGAEVHHA
                     SSQGGEYLNYFLPQCPDGITNVRNSDRLLVSELYDDVDLEYYFDNQGLKYYLIIKPGY
                     SEQNDPISLFYDGADEVNILLDGTLEIKNEVGVLIQNIADAYQIDVDGDRIDLLWDAD
                     FISIDDFEVGFDLGVYDDGLPLVIEMSLTPPGGIADEIDFDNVYWSTVYGSGSGTIQI
                     TDVTYGTYHLPIIIGVNYPLWFPIGTDVTTFGDLFAGGEAYISRFGPLRASNFGVIIG
                     GSGADKPTAIDINNITLDMYISGFTTSNDFYTKDFGGSSYFQDTYTNYIEEGFICQLN
                     ATGNLVRWSTYVEDVVELNSIDFKDDFTEFTVVGQMEGAATASLEELDGAFYEDEEET
                     ALSYIATFNNTNHLIWGTKYWGISAQVGQYDINGRLNIGGQIWGNPYWEDVGDGLGGN
                     GEVFSSPTGGWGENGIYFTTFETDKTVSWATQYGGNGTDFVYSLTSDENGNLYFVGST
                     SKDSYADDMEFLTYSEYEADPDDYFQETYTYLEPVFSFDGYLVKFDDERNRLLSTLFG
                     GPTHEEIYDIEVVNGDIWFVGFTQNESTDFPYTFYDADCFDQSDLMAEKNGFLCIMTD
                     EAVIKYLTYVGWIINSSTYDIAYDNTIDKILTTGAASYNEGDELGFAPVWKPNDFAYY
                     SSLNPAGIAKNSSSFITEWLRLPGDVVIHSDELYKYEIDVYPNPCSSLVNIEAVNLTG
                     EKFVQVFDIRNKKVFEKIYYEDQIDINVGNLAPGFYLGKIQQENSTIDFKFIKL"
     gene            3305..4552
                     /locus_tag="PLL28_02650"
     CDS             3305..4552
                     /locus_tag="PLL28_02650"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HNF68242.1"
                     /translation="MRYIITFFLFFFFLNHGFCQWTLIYEFPEFSDIKDIEINESNEL
                     FIAVDESIYKSSSEGISWDLKYSGSYEIRSIKFFGTDTGYAAKAYGDGTGICLRTVNN
                     GEFWEIPLVFTSYWPDNNDLVLLNGHSIMLAGGQAVGYAQISDDSFETCDVKYPDPTT
                     KSFSAIDCIGGDTCIIIAGDPYGFDDEVGGIFKTEDHGQNWNEKEIVNLGRDIVFTSK
                     EVVYVLGVFDLLKSIDGGNTWNVVKSYNIDKFGEFCAIKFINDSVGYLGEKFNGLDSI
                     AIYFTSNGGLSWSATEIPDLPISLSAIDCIDEKNCYFTTNAGEIYRTINGGMTSISVI
                     DDYIDEVLILPNPINNIVTIYLNNAGLFNPILTNVFGQNCKFEIISRSPDLIKCDISN
                     LTRGIYFISLDDNLNTTFKFIKL"
     gene            4552..4821
                     /locus_tag="PLL28_02655"
     CDS             4552..4821
                     /locus_tag="PLL28_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68243.1"
                     /translation="MGDSFALIVLNLLLLSTISKGQWVKIAEVPHSSLDIQFLDINTG
                     FIIGDQFVFKTADGGYTGDSTELGNSLDLLLEFDFIDADYRIYCR"
     gene            4775..5347
                     /locus_tag="PLL28_02660"
     CDS             4775..5347
                     /locus_tag="PLL28_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68244.1"
                     /translation="MNSILSMRITGYIVGDVNGYQGFYTMDQGGFWLPRTPYILYGQV
                     AMINGNEVVVSIYPPEPAIYHLYFEEALVETLLPGVGGHDIAVFPHDTIYVGGGGFAK
                     SFDKGENWTVTEMPGILHMDFPSSQVGYSTFDDLLFKTTDFGDSWTELELPDYFDACT
                     ALEYANDSVGYYPCTKSASYDGIMKTNDSV"
     gene            5562..6740
                     /locus_tag="PLL28_02665"
     CDS             5562..6740
                     /locus_tag="PLL28_02665"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNF68245.1"
                     /translation="MKEVYIVSIARTPIGSFGGALASVTAPQLGATVIKAAVERAGIK
                     GSDVQEIFMGNVIAANIGQAPVTQAMLAAGLPDTTASTTINKVCASGMKAIMIGAQSI
                     MLGTNDIVVAGGMESMSNVPYYLTKERWGAKLGHSEVVDGVIKDGLWDPYGNKHMGSC
                     GETCAREYKFSREDQDNYAIESYKRAAEAWKTGAFKHEIVPVVIPQRKGDPIVVSEDE
                     DYTKVKFDKVPTLAPAFEKDGTITAANASNLNDGASAVVLMSKERAEAMGIKPLAKIL
                     AFADAQQAPEWFTTTPAKAMPKAIAAAGLQISDIDYFEINEAFSVVALANMKELNISH
                     QKTNIYGGGVSIGHPIGSSGARITITLLSALMNNNARYGCAGICNGGGGASAIVIERM
                     "
     gene            6851..7765
                     /locus_tag="PLL28_02670"
     CDS             6851..7765
                     /locus_tag="PLL28_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68246.1"
                     /translation="MRKSIVAFLLLLISTASYAQKSDKEITPEDMTKLLAYQDTLKWI
                     GDSVVQSPHWEMREQSCIIFLKMLKTALKTPNSYQFAFDSVPTMSVVNAPDNTFRILT
                     WQMQMKDHSHRYYGAIQRNSSALDITPLIDMSMFIGNPEDTLLSANSWYGCLYYNITQ
                     KKYKGKTYYFLFGWDGNDMWSNKKMVDVLTFNEMGVPMFGYPMFLAKDDINPQTRIII
                     EYKEDASPVLNYDEQLKMIVISYLRPENPMSEGIYFTYIPDGTYVGFYFKRGLWRYKD
                     QVFDRTMDAPPDYTPKREGENPNIYKNE"
     gene            complement(7781..8482)
                     /locus_tag="PLL28_02675"
     CDS             complement(7781..8482)
                     /locus_tag="PLL28_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNF68247.1"
                     /translation="MKLKKYRQNYEDFVIEGEKLVTEALLENVYPDLLIATADWLQAN
                     RRSIPEAVQVVETSMADIEKISSLSTPQPVLALMKKKEVSINTIDITQKWSLALDGIS
                     DPGNFGTILRTADWFGIDTVLCSNDCVDLYNPKVIQSTMGSIFRMSVCYFDLHDYLAS
                     CNLPKYAAVLDGEMLDNHIWPEKGVLVIGSESHGISSKVLATCNHAITIPSYGKSESL
                     NAAIATGILLHDIKR"
     gene            8492..11140
                     /locus_tag="PLL28_02680"
     CDS             8492..11140
                     /locus_tag="PLL28_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNF68248.1"
                     /translation="MFYLRLRQHSVNRVTLVCLIAVIAVFVAGCNSTKYLQNDEFLYQ
                     GAELVFTDTSFTGVDTNALRSDLLDLSRQDPNEKVLGLFPVKTWLYTLGDTTIDHYIA
                     YQEQYDTRFLFVFDYDRLLKFITPLSDPKSNFREWLTTKAGEAPVLIDSTIADETAVR
                     MSNYLYNRGFFEGTAVAGYRYDKKRQTGKVIYEVDAGKLYRMRNVYYEIADKGLLNKI
                     NQIPQKSSLAPGEPIDVDLLKAERTRINDYLRNVGYYRFQKEYIYFEVDTASGFDSLD
                     IYVRVSNPVSDTVHHPFRIRNIYVYPNASIDYVDGVTPTNTFHYTDSTKVSRPSKKKL
                     IAQYASRDTTLASASMYSDYLKGKVNKKDALKYYINKDSSSRKYYTLTAAGDLQPVKL
                     YKTRTDYYLINSLDNYSPKAIADNIFINPNGYYSDSLIQRTVASFSSVGVFKYITVQA
                     LEIWDSTSYQQSIDLIVRLDPLQIRTVSYEFNANTTSDYLLGNAINFGYNQKNLFHRM
                     DQLKLSLKGGIETQLGGDVTYINTSELNAGLSLTLPEFMWPIPIQVPKRYFPKTDWRV
                     NFNYIDQINDFTLFNTTIEYNVSIYENSKNDRAQKQHIFKMPIPTLNIVRVPNISDAF
                     REELNANPLLRQSFEEQLILGYGYTLILNTQPTGKHVLDYYLRTSAEFNTPFSDFIKV
                     DADFRTYLNINTSNNIVFRATSGVAIPLAGKNPDAMFNTEVIPYVKQFFTGGAYSVRA
                     FTVRKLGPGAYVDYDTTNFQRVDQVADIKLEANLEYRFDIISMLEGAVFCDIGNTFTL
                     KDDPFRPHAQFHFTDFYKLLAVGPGAGLRLDFGYFVIRVDAAYPLYDPALDGPYREEV
                     LGAYEAAGFTVPSKKVAINLAIGYPF"
     gene            complement(11121..11300)
                     /locus_tag="PLL28_02685"
     CDS             complement(11121..11300)
                     /locus_tag="PLL28_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68249.1"
                     /translation="MKAKNVQLIAGAEGSQNKGPERKAAVPDPEQVGRQREMITICKP
                     APEVRNTRSVKTDIQ"
     gene            11456..13630
                     /locus_tag="PLL28_02690"
     CDS             11456..13630
                     /locus_tag="PLL28_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68250.1"
                     /translation="MLKKLLFPLAVSILVYSSAVAQHYMYNYNVPVLQGTTNYQNPFA
                     GGLNNPQFSSIDINQDGKKDLFLFDRSGNIPMIFINRGGTGEINYEYRAEYASRFPAM
                     RDWAILLDYNCDGLEDIICGYDNGIKTYLAASDGGSINFTEDASKLQFTEAGFFFDLP
                     VGYIDIPGFADIDQDGDIDVITFNMIGGIVDHYDNQAVEDGLPCGTWALEHINSCWGN
                     FYESGITYGVDLNYDCKGIVRTADGLHAGSTFMIFDEDADNDMDVVLGDLAFDVLNKL
                     TNGGDSELAYITDQDTTFPSYDKPYDLPAFPAPFLIDINNDGAKDMVVAPNNINSSEN
                     FKNVWYYQNISTDDTYVFDYRQDTLMVSTMLDFGDGAFPVFFDYNYDGLLDLIVGNYE
                     YFDDGDQIGKIALLQNTGTADAPAYTLITRNLGNISDFGFHGIAPTFGDLDGDGDQDM
                     LIGEEEGFMHLFKNIGPPEGPAEFIFFTGNYQGIDKGQNATPQLFDVNDDGLLDLILG
                     ERNGNINYFENTGTATTPVFTLISEFWGNVDVRSTGVLTGYSAPCMYRSPEGDMTLYV
                     GCEEGTIFQYQPAADITAPFIEVTDHFSDIDCGAFSSVNINEINNDDYPDIICGNKRG
                     GVTLFRDDRSTGIANHEAVSPLIFPNPSTDNIYIDGLQKITGPILISVCSIDGKEVKS
                     TMVSSGNMPVQLSLSDIASGVYVLHITTRQEANISSQLLIKQ"
     gene            13627..15798
                     /locus_tag="PLL28_02695"
     CDS             13627..15798
                     /locus_tag="PLL28_02695"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68251.1"
                     /translation="MIRKLSFTIVLFCSVVVLSAQFKPNTTITASQYGSNLDLAFMGG
                     VTAPQFSDIDVNQDGLMDLFIFDRGAWRPFILLRDISGDYTYAPQYEKIFPHMEDWAL
                     IRDYNCDDVPDIFTYSIGSTAVYKGSFDGGSLHFTLVDDDLTYVDGTGTTSLYTSRTD
                     IPGIADIDQDGDIDVLSFSVSNATIRFYKNISAEQGYGCDSLIYDLVEYCWGEIFEEA
                     SCAGATMNVACKGGGDMSAASEAKIELHIGSTIVTYDTDGDGDHDAMIGDNLCNNIVF
                     FRNGGTTDFAQMSSKDTAFSSTNVSFDLALFPAAYLSDVDDDGDEDMLITTNDHLIGA
                     NMQNVWLYENLNTNDTFDLVYATDTFLVSEFIDAGIYSKPTFFDYNNDDLMDILVGVG
                     STLGKDDVLKHGLWLYENVGTPTSPSFELVSTDYAGLNAYPISQLSPVAGDIDNDGDD
                     DLLIGINDGTICYLQNIGGPTGEAIFTTAVFVYQGIDVGQLASPCLIDIDEDGKLDLV
                     IGEQNGNLNYYHNTGTATSPVFTSESEIWGGVDVRKPGFITGYSSPFMYRNENDSLYL
                     LVGSQSGFVYAFNEIEDALLGEFYEKDTNYLKWDPGVYASIYGQDITGDGEMEFLVGT
                     IRGGIQIFERDKGTAIAEISNEANLQLFPNPSDGNFTITWDQESAALVEVYTMMGNII
                     WSESVYGSALQVNLPESIAKGVYMIRVSYPGARGTLQYIKQ"
     gene            15795..16232
                     /locus_tag="PLL28_02700"
     CDS             15795..16232
                     /locus_tag="PLL28_02700"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNF68252.1"
                     /translation="MISDHFLRLEQLFLKAPIQQNIIGQTISVAEGTALIKYHVNNAF
                     FHGGNAQHGAIYFKLLDDASYFAAASLVSDRFLLTASFEIKLFKPVTEGMLTATGSCK
                     MAGDRIIYAEANLMNEHQELVATGAGRFQRGKQLWASLPGYSG"
     gene            16311..16688
                     /locus_tag="PLL28_02705"
     CDS             16311..16688
                     /locus_tag="PLL28_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68253.1"
                     /translation="MKKLVTLLFVAVSTISITFAGNQGPVTATLDEHMKIVLPTDQPM
                     AQEYVIDIAGRFVSAEVLNSFCDSFRDQGVSYRGDFAAGKMYISVTPLTDSTNHTWDA
                     AKWNEYFSSRSFKMEYFMQSMNK"
     gene            16715..19216
                     /locus_tag="PLL28_02710"
     CDS             16715..19216
                     /locus_tag="PLL28_02710"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HNF68254.1"
                     /translation="MIRQLAITLLAGAAIFTASAQPCIPTYTAGTTDNDYIESFECNT
                     ISNPGSGPGDGSGYTDFTDLSTDLVINETYEIFVENTPYFGENYRIWIDYNHDNIFSS
                     TEEIVTLFSLAAGGSTTLSFTVAMTASPGETTMRIRCVYGTTDFDACSGETYGETEDY
                     AVNIIGLANDVGVSIVNDITDDCDLSSDETVSIIVTNYGTEPASGFSVSYFVDGGTVN
                     TEMFPGGILPGASSPFTFSAGADLSSDGTHVITAWTNYADDMFNGNDSSNVSINNLFT
                     YLTTGFPENICYDGGTIFPSPLAGGGTWSGEAIIDPLTGEMDPTLVGGIGGSTDVTYS
                     FTTTGSYTVTQIPYAPAVPAFETTLGLGDDATANDINIGFPFTYFGNTYTKLFISSNG
                     LIGFSAPSTSYTQQHFPNAADPDNIIAFCWTDLNPSAGGSISYETIGVAPNRKFIVYY
                     DDVVHYLGTATVTGQIVLHETSNAIDIIAVNIESDGGNMTQGIENTNGTEAYNAGEAY
                     NNAVWTMSEMSWRYAVTPCDGEVTETINFVTPPVVELPDSAVCQGSSVTLDAGTGAEY
                     YVWSTGENTQSITVDETDDYWVTYYANEFCFVTDSASIIVNSNPPVDLGPDGTKCEGT
                     MLDAENPGADYLWNTGATTQTLFVTEPGTYSVYVENPITGCYNSDTITMSIIPLPIAD
                     YSAVATDLTVVFTSLAGDVDTWFWDFGDGSTSIEENPWHIYPFAGDYTVTFVVTNGCG
                     ADLISEVFSITTGINALSQNALSIYPNPASTTIHVSGLPNGQEQHFRIMNMTGSVVLE
                     GKSMHDIDISALPASTYVLLVDDGSGQFKGTFVKD"
     gene            complement(19265..19780)
                     /locus_tag="PLL28_02715"
     CDS             complement(19265..19780)
                     /locus_tag="PLL28_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68255.1"
                     /translation="MDAVTAHIVSGAAAGFVGGCVNIYLWQHTLRHKKLLGVKLHKGF
                     AAVVADILQQIPEKFDTKNLKHIEKDILDLFDHFMSDQLTKKMPVLSMFMDEKLIAEI
                     RTIFKLEMESHLPKLLMNRITAEKNIAMVAGLVAKAVRKGLRQYAKHAIIYILLGTLG
                     GALVGYAMSFI"
     gene            complement(19768..20409)
                     /gene="pyrE"
                     /locus_tag="PLL28_02720"
     CDS             complement(19768..20409)
                     /gene="pyrE"
                     /locus_tag="PLL28_02720"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNF68256.1"
                     /translation="MLFQSDDAIVFAEKLLAIEAIKLRPNEPFTWASGWRSPIYCDNR
                     LTLSYPEVRTWIKNTLAKGIRQQFPNVEVIAGVATAGIPHAALVADAMGLPMIYVRSE
                     AKKHGLSNQIEGRCAPGSRVVVIEDLVSTGGSSLTAVAALRNVEAQVLGMAALFTYAF
                     PVADEAFNAASCKLFTLSDYPSLITHAVKNQIIPADTADMLNTWRESPSTWMQ"
     gene            20408..21055
                     /locus_tag="PLL28_02725"
     CDS             20408..21055
                     /locus_tag="PLL28_02725"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNF68257.1"
                     /translation="MPQSYKIFINENPVFLIAGSIPVNANLNDSAHPILNFNQEKEVE
                     KAFHLLENHAGIKSLTIYGDNAKEMRNTLFAGYKKIAAAGGLVFNNKGQVLLIYRRNS
                     WDLPKGKIDAGEKKKAAALREVREETGLEKLSIIRKLKKTYHTYLLENNTPVLKVTHW
                     YLMMCNDVTPPVPQTEEDIELATWIDIDEVELKFNKTYANIQDVIHSGVTMMHYG"
     gene            complement(21052..21516)
                     /gene="coaD"
                     /locus_tag="PLL28_02730"
     CDS             complement(21052..21516)
                     /gene="coaD"
                     /locus_tag="PLL28_02730"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNF68258.1"
                     /translation="MTKIAVFPGSFDPVTLGHINIIERALPLFDQIIIGIGHNSTKSG
                     FFSLEQRVNWLQEIFLHEKKVQIHAYEGLTIDFCRHNHAQFLLRGLRSTADYNYELAI
                     AQTNKSLAPEIETYFLMSDPAHAHINSSIVREVYKNGGDISSMVPQIVISGN"
     gene            complement(21532..22089)
                     /locus_tag="PLL28_02735"
     CDS             complement(21532..22089)
                     /locus_tag="PLL28_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HNF68259.1"
                     /translation="MRIIAGKFRGRRFHPPANIPARPTTDFAKEGLFNILTNNFNFED
                     VAYLDLFCGTGSLCYEMASRGCTDIVGVEMDNRSVDFIRKTSTELNITINVLRMDVFE
                     YMRTTKKKFDLIFAGPPYPLPNIPELPEEVFANQLLNPEGWFVLETNPKYSFTDHPHF
                     LRSRNYGTTIFHIFQAEVVKRGDAV"
     gene            complement(22080..22922)
                     /locus_tag="PLL28_02740"
     CDS             complement(22080..22922)
                     /locus_tag="PLL28_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HNF68260.1"
                     /translation="MVHFVDTDFNKDFTSHYSLLFQLEEHQYTYAVYDKRSGKLQLLK
                     TVAFNSAFAPEAFSKFKVSVTAEDALQAMYKEVKIGVVDSAFTLLPRTIFEEEKAKAY
                     LSLTSPIDDQSTVLVNQVRGIFVRNVFSLPLAISSYLDEVFTHPKWFHASTALMETAI
                     RNRDQFADQQIILDIKPGVMYLMYYAKKEFVFMNQFKYVNKDDFLYYVLLMADQFEID
                     REHCDLKLSGEILPDSMLYNELWKFFSNISFLQPNEHIILPDAVREKPVHMFNSLFSL
                     DLCE"
     gene            23001..23969
                     /gene="hemB"
                     /locus_tag="PLL28_02745"
     CDS             23001..23969
                     /gene="hemB"
                     /locus_tag="PLL28_02745"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNF68261.1"
                     /translation="MYSRPRRLRSNAIMREMVAETRFSVQSLMYPMFVTHGKGINHPI
                     GAMPGISHFSPDTLVKEVEHLMSLGINKILLFGVGEDKDPVGSAAYDFTNAVANGVRS
                     LKAAFGNDLLIATDVCMCAYTDHGHCGILHGHEVANDETLTSLAKMALMHAQAGADIV
                     APSDMMDGRVAAIRDTLDAENMQQVAIMSYSVKFASGYYGPFREAADSAPSFGDRKTY
                     QMDVRNSREAIKEALLDVEEGADILMVKPALAYMDLIRTVKDQTLLPLACYNVSGEYS
                     MVKAAAEKGWIDEQRIVMENMASFTRAGADIIITYHAKDMLSKGWV"
     gene            24105..25385
                     /locus_tag="PLL28_02750"
     CDS             24105..25385
                     /locus_tag="PLL28_02750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNF68262.1"
                     /translation="MVSSLFNNLISLIGVAFMGRVGETELAACGVAALIYITITMIPY
                     GLSVGFQIIIARRAGENNPESIGRIFNNNLIFLMGVAVLLYAFLQFMAPILLKPMFSS
                     PEVYAAGMTYLKYRNIEIFFAVFAFTLLGFYTSVGQNQIISYSAILMLVVNVFFSYNL
                     VFGGMGLPEMGIGGAGLANLISASAACLLNFGYLFVSPLRARFGLFKFDKFDWPIVKK
                     GLELSGPVVLQHMLSTGTWVIFFLMIEKMGPPALAASNVAKEVYMVLGVTTWGVANAT
                     NSLVSNLLGQKRHDEMFDLIKKVVLIALAFSFAFFLFIILFPQVFIGIFTNDPTITAL
                     ATGPVRIVGTCLVMMAVSSVLFRAVTGTGATRYSLLLELITLIVYMVYVIIIIKVLDV
                     NLTIAWTSEFVYWLVLAWLCWTYLKSGAWKRHRV"
     gene            25516..26322
                     /locus_tag="PLL28_02755"
     CDS             25516..26322
                     /locus_tag="PLL28_02755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68263.1"
                     /translation="MKKNAFVWVFGLITISVVGTSMSYQNPNNPPLGYTGAPGEQTCQ
                     KASCHGGGNFVGTPTLSGLPDSIEPGASYSLTLTLESNCTKAGFELTCLDADDVKAGT
                     FSAGTGNSVANFGGKQYVRQSSPKTLTGGSTSWTFTWHAPETAGGDAVTFYFASLNAN
                     GNGNDSGDKAIKGDTTIAYKVPEVILDATEIAKPEITVTPNPATDHISINHAAVGSTM
                     QLFDIHGKLIMSKHINAVSVTYSTAELPAGNYLLAVSNNGERTSKQISIQ"
     assembly_gap    26506..26605
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7755 a         5118 c         5909 g         8070 t
ORIGIN      
        1 cggcactatg gacttcacga tatgattatt catctgatca agaagttgca gcaatgatta
       61 aaaatggccc ttcaagtagc gtcgttgtta ttggagcaag cgaaaattca ccaggaagtt
      121 gggattttgc agctgtaaaa tatagtcaga gcactggatc agttgtagct acgaatcgta
      181 attcagcatc tggtagtggc tttgatcagg tattcggagc cgatgtcgac gcttcaggaa
      241 atttttatat aacaggacgt gctgctgtcg tagatgaagg ttttaatatg cgaacagtta
      301 aaattgatcc atctatggat ttagtctggg caaaaaatta tgatcacgca ggactagacg
      361 atgaatctca tggagttgtg gttgatctag atgataatgt ttacgtaaca ggatggatta
      421 caaatgagga tggaacaagg tcatttgaaa caattaaata taatagcagt ggcaccccac
      481 agtggcatca ggaggtgaat gcagaaaatg ttggattaga tgcatatagt ctaaagataa
      541 gtagtgctgt tgatggaaat attattgttg cgggtaatgt tgacaatggg gcgagcttag
      601 attatttaac ggttatttat aacgaagatg gtgatcagct gtggatggaa cagtatgaca
      661 gtccagatgg ggatgatgat aaagtgaatt ttgtaaaagc tgatattgga aacgctgttt
      721 acataggggg taaatcatat tctatttcta ctgcaacaaa tagattggtt aaatataatt
      781 gggaagatta tatagtgcct ccagacgacg acttagatca tccttcgcaa tttacctttt
      841 ttgaaaatcg cggtcagatt attgacactg atgaaaatcc tgttaacgat ataaaatatt
      901 atactttcca tcaaacccct ggcttgtttg tgtttgatga taaaataagt tatgtatggg
      961 gaagaattga tacaacaaca aatactgatg atactttatc gagaattgat cttagttttc
     1021 cggattgtag agtaggcgct gaggttcacc atgcatcaag tcagggtggt gaatatttaa
     1081 attatttttt acctcaatgc cctgatggaa ttacaaatgt gagaaatagt gataggcttt
     1141 tagtatctga attgtatgat gatgtggatc tggaatacta ttttgataac caaggtctta
     1201 aatattatct tattattaag ccaggttata gcgagcagaa tgatcccatt tcgttatttt
     1261 atgatggagc cgatgaagta aatatacttt tagatggtac acttgaaatt aaaaatgaag
     1321 taggtgtatt aattcagaat atcgctgatg cataccagat agatgttgat ggagatagaa
     1381 ttgatttatt atgggatgcg gattttattt caattgacga ttttgaagtt ggctttgact
     1441 taggggtata tgatgacggt cttccgttag tcattgaaat gtcgttaact cctccgggag
     1501 gtatagcaga tgaaattgat tttgacaacg tttattggag tacggtttat ggatcagggt
     1561 caggaactat tcagattaca gacgtcactt atggtacata tcatttacca attattatag
     1621 gtgtcaatta tccattatgg tttcctatcg gaactgatgt tacaactttt ggtgacttat
     1681 ttgcaggagg agaagcatat ataagtcgat ttggcccatt gagggcaagt aattttgggg
     1741 taattattgg tggttcagga gcggataaac cgactgctat tgatataaat aacataacat
     1801 tagatatgta tatatctgga tttactacaa gcaatgattt ttatacaaaa gattttggag
     1861 gttcttctta ttttcaagat acatatacaa attatataga ggaaggattt atttgccaac
     1921 taaatgctac aggaaatttg gtaaggtggt ctacctatgt tgaggatgtt gtagagttaa
     1981 atagtataga tttcaaagat gattttacgg aattcacagt tgtaggacaa atggaaggcg
     2041 ctgcaacagc atcgttagaa gaattggatg gggcttttta tgaagatgaa gaggaaacag
     2101 ccttgagtta tatagctaca tttaacaaca caaatcatct tatttgggga acaaaatatt
     2161 ggggaatttc agctcaggta ggtcagtatg atattaatgg taggttaaat atcggaggtc
     2221 aaatatgggg caatccttat tgggaagacg ttggtgatgg attaggcgga aacggagaag
     2281 ttttttcatc tcctaccggc ggctggggtg aaaatggaat ttattttacg acttttgaga
     2341 cagataagac tgtatcatgg gctactcagt atgggggcaa cggcactgac tttgtttata
     2401 gcttaacctc tgatgaaaat gggaatttat attttgtagg gtcaacatct aaagactctt
     2461 atgcggatga tatggaattt ttaacatatt cagaatatga agcagatcct gacgactatt
     2521 ttcaagaaac ctatacctat ttagaaccgg ttttttcctt tgacggatat cttgttaagt
     2581 tcgatgatga aagaaatcgg ctgttatcta ctttgtttgg aggaccaaca catgaggaaa
     2641 tttatgatat tgaggtggtc aatggggata tttggtttgt tggattcaca caaaatgaaa
     2701 gtacagattt tccatatacc ttttatgatg ccgattgttt tgatcaaagt gatttaatgg
     2761 cagaaaaaaa tgggtttctc tgtataatga ctgatgaagc tgtcattaaa tatttaacat
     2821 atgttggatg gataattaat tcttcaacct atgatatagc atatgataac accattgata
     2881 aaatcttaac aactggtgct gcttcctata atgaagggga tgaacttggc tttgccccgg
     2941 tctggaagcc aaatgatttc gcatattatt cgtcattaaa tccagcagga attgcaaaaa
     3001 attcaagctc tttcataacc gaatggctga gattaccagg tgatgtggtg attcattctg
     3061 atgaactcta taagtatgaa atagatgtat acccaaatcc ttgttcctct ttagtcaata
     3121 tagaagctgt taatttaact ggggagaaat tcgtacaagt gtttgatatc agaaataaga
     3181 aagtttttga gaaaatctat tatgaagatc agatagatat aaatgtgggt aatttggcac
     3241 ccggttttta ccttggaaaa atacaacaag aaaactccac gattgacttt aagtttatta
     3301 aattatgaga tatataatca ctttttttct atttttcttt tttctaaatc atggtttttg
     3361 tcaatggaca ttgatttatg aatttccaga attttccgat ataaaggata ttgaaattaa
     3421 tgaatcaaat gaattattta tcgcggtcga tgaaagtatt tataagagtt cttctgaagg
     3481 aatttcttgg gatttgaaat attcaggttc gtatgaaata cgaagtatca aattttttgg
     3541 tactgatacc gggtatgccg ctaaagcata tggtgatgga actggtatat gtttaaggac
     3601 tgtaaataat ggagaatttt gggaaattcc tttggtattt acatcatatt ggccagataa
     3661 taatgatttg gttctgttga atgggcattc aataatgctt gccggggggc aagccgtggg
     3721 atatgctcaa atttcagatg attcgtttga aacttgtgat gtaaaatatc cggatccaac
     3781 cactaaaagt ttttcagcta tcgattgcat cggaggtgac acgtgtataa taattgcagg
     3841 agacccatat ggttttgatg atgaggtcgg agggattttt aaaacagaag atcatggcca
     3901 aaattggaat gaaaaagaaa tagttaatct tgggagagac atagttttta cttcgaaaga
     3961 ggtggtatat gttttgggtg tttttgattt attaaaatca attgacggtg gaaatacgtg
     4021 gaatgttgta aaatcgtata acattgacaa atttggtgaa ttctgtgcaa taaaatttat
     4081 taatgacagt gtgggctatc tgggtgaaaa atttaatggg ctagatagta tagcaattta
     4141 ttttacttca aatggaggtc tttcatggag tgcaaccgaa atcccggatt tacctatttc
     4201 tctatcagca attgattgca ttgatgagaa aaattgttat tttacaacta acgcgggtga
     4261 aatttataga actataaatg gtggaatgac ctctatttcc gtaattgatg actatattga
     4321 tgaagtatta attttaccaa atcccattaa taatattgtt actatttatt taaacaatgc
     4381 tggattattt aatcccatac ttactaatgt atttggtcaa aattgcaaat ttgaaattat
     4441 ttcccgtagt cctgatttaa taaaatgtga tatttcgaat ttaaccagag gaatatattt
     4501 tatatcactt gacgataatt taaatacaac gttcaaattt attaaattat aatgggtgat
     4561 tcatttgcgt tgatagtttt aaatctattg ctcttatcga ctatttcaaa agggcaatgg
     4621 gtaaaaattg ctgaagtacc tcacagctca ttagatattc agtttttaga tattaatact
     4681 ggttttatta ttggggatca atttgtattt aaaactgctg atggtggtta taccggggat
     4741 tcaactgaac taggaaactc actagatctt ttgcttgaat tcgattttat cgatgcggat
     4801 tacaggatat attgtaggtg acgtaaacgg ttatcaaggg ttttatacga tggatcaagg
     4861 aggattttgg ttgccaagaa ccccgtatat tttgtatggg caagttgcta tgataaatgg
     4921 caacgaggta gttgtgtcaa tataccctcc cgaacctgcg atctatcatc tttattttga
     4981 agaggctttg gtagaaacgt tattacctgg tgtgggtggt cacgatatag ctgtttttcc
     5041 gcacgatact atttatgtcg gcggtggtgg atttgccaag agttttgaca aaggtgaaaa
     5101 ttggactgtc actgaaatgc cgggaatttt acacatggat tttccttctt cgcaagttgg
     5161 ctattccacc tttgatgact tgctatttaa aacaactgac tttggtgatt catggactga
     5221 attagagttg ccagattatt tcgatgcttg cactgcgctt gaatatgcca acgattctgt
     5281 aggctattac ccttgcacta aaagtgcttc ttatgacgga attatgaaaa ctaatgattc
     5341 agtctgacaa ccaacatcgc ggacaacagc tcatcaccat tgtgaaagcc acgtggggtg
     5401 ttttgctggt cattgcattc ctgcttcttg cccagtgaac ttttccggcc actttttggt
     5461 agctgaaaag gtacgcagga tatgtctcag catgatgcta gtcttttcga cctcctcatg
     5521 gtaaaatccg tatctttgcg gcgaaattca cagttaacat tatgaaagaa gtatatatcg
     5581 tttcaatagc ccgtacgcca attggtagtt tcgggggagc tttggcctct gtaacggctc
     5641 cacaactggg tgcaactgtt atcaaagccg ccgtagaacg cgccggcatt aagggtagtg
     5701 atgtgcagga gatctttatg ggtaatgtaa ttgcggctaa tatcggtcag gctcctgtta
     5761 cacaagctat gcttgctgca ggacttccgg acaccacagc ctctaccacc atcaacaagg
     5821 tatgtgcttc aggaatgaag gccattatga tcggtgcgca atctatcatg ctgggaacca
     5881 atgacatcgt agttgcgggt ggtatggaga gcatgagcaa tgtgccctat tacctcacca
     5941 aagagcgctg gggtgcgaaa ctgggacata gcgaagttgt tgatggtgtg attaaagacg
     6001 gtctctggga tccttatggg aacaaacaca tgggttcatg cggcgaaaca tgtgcccgcg
     6061 agtataaatt ctctcgtgaa gaccaagaca attacgctat tgagagctac aaacgtgctg
     6121 cagaagcatg gaaaacaggt gctttcaaac atgaaatcgt tccggttgtg atcccacagc
     6181 gaaaaggcga cccgatagtg gtttcagagg atgaagatta cacaaaagtt aagttcgata
     6241 aggttcctac attggctccg gctttcgaaa aagacggaac tatcaccgca gccaatgcca
     6301 gcaacctcaa cgatggtgct tcagcggtgg tgttgatgag caaggaacgc gcagaagcca
     6361 tgggtatcaa gcctttggcg aaaatattgg cctttgctga tgcccaacag gcaccggagt
     6421 ggttcactac tactcccgca aaagcaatgc caaaagctat tgcagcagcg ggtttgcaga
     6481 tcagtgatat cgattatttc gagatcaacg aggccttttc agttgtagca ctcgccaata
     6541 tgaaggaatt gaacatttca caccagaaaa ccaatatcta tggtggtggc gtttcaattg
     6601 gtcaccctat cggttcttcc ggagcacgca tcaccattac cttgttgagt gccttgatga
     6661 ataacaatgc acgctatggt tgtgccggta tctgtaatgg tggcggtggc gcgagtgcca
     6721 tcgtgattga acgcatgtga ttggagtaaa gcatggatta ttcacatcct gcgacacaat
     6781 aaaagagatg aaatttgctt tataatgcct gagttttgca ctcgggcatt tttttcgcca
     6841 aacctgaact atgcgtaaat ctattgtagc ctttttactg ctgcttattt caacggcatc
     6901 ctatgcacaa aaaagtgata aggaaataac gccggaggat atgacgaagc tattggccta
     6961 tcaggacacg ctcaaatgga taggtgattc ggttgtacag agtccgcatt gggaaatgcg
     7021 cgagcaatca tgcattatct tccttaaaat gctgaaaacc gccctcaaaa cccccaattc
     7081 ataccaattt gcctttgatt ctgtacctac aatgtcggtc gtaaatgcac ctgacaatac
     7141 attcaggata ttgacctggc aaatgcagat gaaggaccat agtcatcgtt attatggtgc
     7201 catacagcgc aattccagtg cgttggatat tactccgctc atcgacatga gtatgtttat
     7261 agggaatccg gaggatacac ttttatcggc aaacagctgg tatggctgtc tgtattataa
     7321 catcacccaa aagaaataca agggcaagac ctactacttt ttatttggtt gggacggcaa
     7381 tgacatgtgg agcaataaaa agatggtaga tgtgcttaca ttcaatgaaa tgggtgttcc
     7441 gatgtttgga tatccgatgt ttttggcgaa ggatgacatc aacccacaga cgcgcataat
     7501 catagaatac aaagaagatg cctcgcctgt gttgaattac gatgagcagc taaaaatgat
     7561 tgtgatcagc tacttgcgac cggaaaaccc gatgtcggaa ggaatttact tcacctatat
     7621 tcccgacggc acctatgtgg gcttttattt caaacgaggt ctttggcgtt ataaggatca
     7681 ggtgtttgac agaactatgg atgcacctcc ggattacacg cctaagcgcg aaggtgagaa
     7741 cccgaatatt tataaaaacg agtaatcagc aaagcgagat ttatcgctta atatcatgca
     7801 gtaatatacc tgttgcgata gcggcattta ggctttcaga tttaccgtat gaagggattg
     7861 taatggcatg attgcaagtt gccaatactt ttgatgatat gccgtgagat tcgctcccga
     7921 tgaccagcac tcccttctca ggccaaatgt ggttatcgag catttcgcca tcaagaacag
     7981 cggcgtattt tggaagattg caagatgcaa gataatcgtg tagatcaaaa taacataccg
     8041 acatacgaaa tattgaaccc atggtactct gaatcacttt gggattatac aggtcgacgc
     8101 aatcattgct gcaaagaacc gtatcaattc caaaccaatc tgccgttctc agtattgttc
     8161 cgaagtttcc cggatcactg atgccatcta aagcgaggct ccatttttgg gtaatatcga
     8221 ttgtgttaat ggatacttct tttttcttca tcagtgccag cacaggttga ggcgtgctga
     8281 gtgatgatat tttttctatg tccgccattg aagtttcaac aacctgaacg gcttcgggaa
     8341 tagatcttcg attcgcctgt aaccaatcgg cagtggctat taataggtca ggataaacgt
     8401 tttccaacaa ggcttccgtt actaacttct cgccttcaat cacgaaatcc tcataatttt
     8461 ggcgatactt ttttagtttc aagccctgga gatgttttat ctgcgccttc gtcaacatag
     8521 cgttaaccgc gtcacactgg tctgcctcat cgcggtaatt gcagtgtttg ttgcgggctg
     8581 caattctact aaatatctgc aaaacgacga attcttgtat cagggagccg agttggtgtt
     8641 tacggatacc agctttaccg gtgttgatac aaatgccctg cgatcagatc tgttggattt
     8701 aagtcgtcaa gaccccaatg aaaaggttct aggtcttttt ccagtcaaaa catggttata
     8761 tacgctgggt gacacgacaa tagatcatta tatcgcttat caggagcaat atgacacgcg
     8821 attcttattt gtgttcgact acgatagact attaaaattc atcactcccc tctccgaccc
     8881 taagagtaat tttcgcgaat ggcttacaac caaggccgga gaagctcccg tgttaataga
     8941 tagcacgatt gctgatgaaa cagcggtgcg catgagtaat tatttataca atcgcggatt
     9001 ttttgagggc actgctgttg caggatatcg gtatgacaag aaaaggcaaa ccggtaaagt
     9061 gatatatgaa gttgatgccg gcaagttata tcgcatgcgg aatgtttatt atgaaattgc
     9121 cgataaaggc ttgttgaata aaataaatca aattcctcaa aaaagcagtc tggcacccgg
     9181 tgaaccgata gatgttgatt tgttgaaagc cgagcgcacc cgcattaacg actatctgcg
     9241 taatgtaggt tattaccggt ttcaaaagga atacatctat tttgaggtag atactgcttc
     9301 cgggtttgac agtctggata tttatgtgcg cgtttcaaat ccggtgagtg ataccgttca
     9361 ccaccctttt cgcatcagga atatttacgt ttatccgaat gcctctatcg actatgtgga
     9421 tggtgtgaca ccaactaata cttttcacta taccgatagc acaaaagttt ccagaccatc
     9481 taaaaagaaa ttgattgcac aatacgcaag cagagacacc acacttgctt ctgcaagtat
     9541 gtatagtgac tatttaaaag gcaaggtaaa caaaaaggat gccttgaagt actatatcaa
     9601 taaagattca tcatcgagga aatattatac cctaacggca gccggtgatt tacagcctgt
     9661 aaaattgtat aagaccagaa cggattatta tttgatcaat tcgctggata attatagtcc
     9721 gaaggccatt gcggataata ttttcataaa ccccaacggc tattattccg attcattaat
     9781 tcagcgcaca gttgcttctt tttcgtcggt tggcgtattt aaatacatta cggtgcaggc
     9841 cttggaaatt tgggattcta ccagttatca gcaaagcata gatttgattg tgcgattaga
     9901 tccattgcaa attcgcacgg tatcgtatga atttaatgcc aacaccactt cagattactt
     9961 attaggaaac gccattaatt tcggttacaa ccagaaaaat ctatttcata gaatggatca
    10021 actgaaactg agtttaaaag gaggtattga aacgcaatta ggtggtgatg tcacctatat
    10081 caacacctcg gaactcaatg ccggcttgag tctcacgcta cctgaattta tgtggccgat
    10141 accgatacaa gtgccgaaac gctattttcc aaagacagac tggcgagtaa atttcaatta
    10201 tattgatcag atcaatgatt tcactttatt taataccacg atcgaatata atgtttccat
    10261 ttacgagaat tccaaaaacg atcgtgctca gaagcagcat attttcaaaa tgccgattcc
    10321 tacactgaat atcgtgcgcg taccaaatat cagcgatgcc tttcgtgagg aattgaatgc
    10381 aaatccatta ttgcgtcaat catttgaaga gcagctcatc cttggatacg gatatacact
    10441 gattttaaat acccagccga caggcaagca tgtgctcgat tactacctcc gcacgagtgc
    10501 cgaattcaat acaccattca gtgattttat caaagtagac gcggattttc gtacctatct
    10561 gaatatcaat acttccaaca atatcgtgtt cagagcaacc tcaggtgtag ctattccgct
    10621 tgcaggtaaa aatcccgatg ccatgttcaa cacagaagta attccttacg taaaacagtt
    10681 tttcaccgga ggggcttaca gtgtcagagc gtttactgtg cgcaagcttg gtcccggtgc
    10741 ctatgttgat tacgatacga caaatttcca gcgcgtagat caggttgctg atattaagct
    10801 cgaggccaat ctcgaatacc gtttcgatat catctccatg ctggaagggg cagtattttg
    10861 tgatatcggt aacacgttta cgttaaaaga tgacccattc cgaccacatg cacagtttca
    10921 tttcactgat ttctacaaac tgctggctgt tggaccagga gccggattgc gactggattt
    10981 cggttacttc gtgatacgtg ttgatgccgc ttatcccttg tatgatccgg cattagatgg
    11041 tccgtatcga gaagaggtat taggcgcata tgaagctgcc ggttttaccg ttccttcgaa
    11101 aaaagttgca atcaatctgg ctattggata tccgttttaa cggatcgggt gttgcgcact
    11161 tccggagcag gtttgcaaat agtaatcatt tcgcgctgcc tgcccacttg ctctggatca
    11221 ggaacagctg ctttgcgctc gggccctttg ttttgcgagc cctctgcgcc tgcgataagt
    11281 tgcacatttt tcgctttcat agctcgcttt tttattgtga tgtaatagca tgttactatc
    11341 cataaataga attccgtgcc aaactttaca aacccctatc accgctgtgt caaaaacgct
    11401 gatgctagca acaaaacaac atgcttgttg tatctttatc attcattctg tgcgcatgtt
    11461 aaaaaagctc cttttccctt tagcagtaag cattttggtt tatagcagtg ctgtagccca
    11521 gcattatatg tataactaca atgttccggt attgcaaggg acaacaaatt atcagaatcc
    11581 ctttgcaggt ggattgaata acccgcaatt ttcatccata gacataaatc aggatggaaa
    11641 aaaggatttg tttcttttcg acaggagtgg aaatataccc atgatattca tcaaccgagg
    11701 tggcacaggc gaaatcaatt atgaatatcg cgctgaatac gcttcacgat ttcctgctat
    11761 gcgagattgg gcaattttgc tggattataa ttgcgatgga ttagaagata ttatatgcgg
    11821 ctatgataat ggaataaaaa catatcttgc tgcatcagat ggaggtagta taaattttac
    11881 ggaagatgcg agtaaattgc aattcacgga agcaggattt ttctttgatc ttcctgttgg
    11941 ttatatagac attcccggtt ttgcagatat cgatcaggat ggtgatatcg atgtgatcac
    12001 ttttaatatg atcggcggca ttgttgatca ttatgacaat caagccgtgg aagatggttt
    12061 accatgcggc acttgggcat tggaacatat caattcctgc tggggaaatt tttatgaaag
    12121 cggaatcacc tacggtgttg atcttaatta cgattgcaaa ggaattgtgc gcactgccga
    12181 tggtctgcac gcaggatcta catttatgat attcgatgaa gatgctgata acgacatgga
    12241 tgttgtactt ggcgatcttg catttgatgt attaaataaa ttaacaaacg gcggcgatag
    12301 tgaattggca tatattactg atcaggatac cacctttcca agctacgaca agccatatga
    12361 tttacccgca tttccggcac catttctaat tgatatcaat aatgatggag cgaaagatat
    12421 ggttgtagca ccaaataata tcaatagttc agagaatttt aaaaatgtat ggtattatca
    12481 aaatatttct accgatgata cttatgtgtt cgattataga caagatacgt tgatggtgag
    12541 cactatgctt gattttggcg atggcgcttt tcctgtgttt ttcgattata attatgatgg
    12601 actattagac ctcatagtag gcaattatga atatttcgac gatggcgatc aaattgggaa
    12661 aatagcattg ttacaaaata ccggcactgc cgatgcgcca gcatatacac tcattacgcg
    12721 caacctcgga aatatttccg atttcggatt tcatggaata gccccaactt ttggcgatct
    12781 ggatggcgac ggcgatcagg atatgctgat aggtgaagaa gaaggtttta tgcatttatt
    12841 taaaaacata ggaccaccgg aaggacctgc cgaatttatt ttcttcacag gtaattatca
    12901 aggtatagat aaagggcaaa atgccacacc acaattgttt gacgtaaatg atgatggact
    12961 actcgatctc attttaggtg agcgcaatgg caatattaat tatttcgaaa atacaggtac
    13021 ggcaaccaca cctgttttca cactgataag tgaattctgg ggaaatgtgg atgtgcgcag
    13081 tacaggcgtg ctaaccggct attcagcgcc atgtatgtat cgttctcctg aaggcgatat
    13141 gaccttgtat gttggatgcg aagaaggtac catattccaa tatcaacctg cagcagatat
    13201 cacagctcca tttattgaag ttaccgatca tttcagcgat atagattgtg gcgctttcag
    13261 cagtgtcaat atcaatgaaa taaataatga tgattacccg gatatcattt gtggtaacaa
    13321 acgcggaggc gttacacttt tcagagatga cagatcaacc ggtattgcta atcatgaagc
    13381 tgtttctcct ttaatattcc ccaacccgtc tactgataat atttacattg atggattgca
    13441 aaaaataacc ggtccgatat taattagtgt ttgcagtatc gacggtaagg aagtgaaatc
    13501 aacgatggtt tcatccggta atatgccggt tcaattatcg ctcagtgata ttgcaagtgg
    13561 ggtttatgta ttgcatataa ctacaagaca ggaagcaaat atttcttcac aattattgat
    13621 caaacaatga taagaaaact atctttcacc attgttttat tttgctctgt tgtagtatta
    13681 tcagcgcagt tcaaaccgaa taccactatt acggcatctc aatatggcag caaccttgat
    13741 ctggcgttta tgggaggtgt taccgctccg caattttcag atatagatgt gaatcaggat
    13801 gggttgatgg acctgtttat ttttgacaga ggtgcatggc gaccattcat tttattacgc
    13861 gatatttcag gtgattatac ttacgcacct caatatgaaa agatatttcc gcatatggaa
    13921 gattgggcat tgattcgcga ttacaattgc gatgatgttc cggatatttt cacctattct
    13981 atcggcagca ctgctgttta caaaggaagt ttcgatggtg gctcgttaca ttttacattg
    14041 gttgatgatg atcttaccta tgttgatgga acaggcacaa catcattata caccagcaga
    14101 acagacattc cgggtattgc cgacattgat caagatggtg atatagatgt gttgtcgttc
    14161 agcgtttcca atgcaaccat acgtttttat aaaaatattt cagcagagca aggatatggt
    14221 tgcgattcac ttatttacga tcttgtagaa tattgttggg gagaaatttt tgaagaggca
    14281 tcatgtgcag gcgcaaccat gaatgtggca tgcaaaggcg gaggtgatat gagcgcagcc
    14341 tctgaggcga aaattgaatt gcatatcggt tctacaattg tgacctatga taccgacggc
    14401 gacggcgatc acgatgccat gattggcgat aatttatgca ataatatcgt gttcttcaga
    14461 aatggaggca ccacagattt tgcccaaatg tcgagcaagg atactgcatt ctccagcacc
    14521 aatgtctcct ttgatttagc attatttcct gctgcctatt tatcagatgt tgatgatgac
    14581 ggcgacgagg atatgctgat aaccactaat gatcatttga taggcgctaa catgcaaaat
    14641 gtctggttat atgaaaatct gaataccaac gacaccttcg accttgtata cgcgactgac
    14701 acttttttag taagtgaatt catagatgca ggaatctata gcaagcctac ttttttcgat
    14761 tataataacg atgacctgat ggatattttg gttggcgttg gaagtacctt aggtaaagat
    14821 gatgttttaa aacacggact ttggctttat gagaatgtag gcacaccaac atcaccatcg
    14881 tttgagcttg tttcaaccga ttatgcagga ttaaatgcct atccgataag tcagctcagt
    14941 ccggtggcag gcgatattga caatgatggc gacgacgatt tattaatagg tattaatgac
    15001 ggtaccatct gctatcttca aaatattgga ggcccgaccg gtgaggctat atttacaact
    15061 gcagtttttg tatatcaggg tatcgatgtc ggtcagcttg caagtccctg tttgattgat
    15121 attgatgagg atggcaaact tgatttagtg attggagaac aaaatggcaa tttaaattac
    15181 tatcacaata ccggcacggc aacatcaccg gtttttacaa gtgaaagtga gatttggggt
    15241 ggtgttgatg ttcggaaacc tggatttatt acagggtaca gctctccctt catgtatcgg
    15301 aatgaaaatg atagtttgta tttgttagtt ggttcacaaa gcggattcgt ttatgcgttc
    15361 aatgaaattg aagatgcatt actaggtgaa ttttatgaga aagacactaa ctatttgaaa
    15421 tgggatccgg gtgtttatgc atcaatttac ggacaggata tcaccggtga cggcgagatg
    15481 gaatttcttg ttggcactat tcgaggtggc atacagattt ttgaacgaga taagggaact
    15541 gcaatcgcag agatcagcaa tgaagcgaat ttgcaattat tcccgaatcc ttcagatggt
    15601 aatttcacca taacatggga tcaggaatca gccgcgcttg ttgaagtgta tacgatgatg
    15661 ggtaatatca tttggtcaga aagcgtttat ggaagtgctc tccaagtaaa cttgccggaa
    15721 tccattgcta agggtgtata tatgataaga gtttcttatc caggcgcaag aggaacactg
    15781 caatacatta agcaatgatc agcgatcatt ttctaagact ggagcaacta tttctcaaag
    15841 ccccgataca acaaaacatt atcgggcaga cgatttcggt agcagaagga actgctttga
    15901 tcaaatacca cgttaacaat gcctttttcc atggagggaa cgctcaacat ggagccatat
    15961 acttcaaact gctggacgac gcctcctatt ttgctgcagc ctcattggtt tcggaccgct
    16021 ttttgttaac tgcgtcgttt gaaataaagc ttttcaagcc ggttacagag ggcatgctga
    16081 ctgctaccgg gagctgtaaa atggcaggtg accgaatcat ttatgcggaa gccaacttga
    16141 tgaatgaaca tcaggagctt gtagccacag gtgcaggccg ctttcaaagg gggaagcagc
    16201 tctgggcttc actgccgggc tatagcggat gactatctct caggtatcct tacctccgga
    16261 ctttgcatgt tactggcaga ttggtatctt taagctgtaa agtcactgat atgaaaaaac
    16321 tcgttactct tctttttgtt gctgtgagca ctatttccat aacatttgcc ggcaatcaag
    16381 gtcctgttac tgctacgctc gacgaacaca tgaaaattgt gttgcctaca gatcagccta
    16441 tggcgcagga atatgtaatc gacattgcag ggcgatttgt tagcgcagaa gtattgaact
    16501 ctttctgcga cagcttccgc gatcagggag tgagttatcg cggtgatttt gctgcaggta
    16561 aaatgtatat cagcgttaca ccgcttaccg acagcactaa tcatacatgg gatgccgcta
    16621 aatggaatga atatttctcc tctcgttcct ttaagatgga atacttcatg cagagcatga
    16681 ataaataata gttcattatt aaacctcaac acacgtgatc aggcaacttg caattacctt
    16741 gcttgccggt gcagctattt ttactgcttc agcacagcct tgtattccca cttacactgc
    16801 cggtactacc gacaatgatt atattgaaag ttttgaatgt aataccattt cgaatccagg
    16861 cagcggcccc ggtgatggtt caggatatac ggatttcaca gatttaagta ctgatcttgt
    16921 tattaatgag acttatgaaa tctttgtaga gaatacacct tatttcggcg agaattaccg
    16981 catttggata gactataatc acgacaatat attttcctca accgaagaga ttgttactct
    17041 tttttcttta gccgccggag gttcaactac actgagtttt accgttgcga tgaccgcatc
    17101 acccggtgaa accaccatgc gtattcgctg tgtttacggg actaccgact ttgatgcctg
    17161 cagtggtgaa acatatgggg aaactgaaga ttatgctgta aacattattg gattagcaaa
    17221 cgatgtaggc gtatcaattg taaatgatat taccgatgat tgcgatttat catccgatga
    17281 aacagtaagt attatcgtaa ctaattatgg tacggaacct gcatcaggtt tttcggtgag
    17341 ctattttgtt gatggtggca ctgtgaacac agaaatgttt cccggcggta ttttacccgg
    17401 ggcctcttca ccattcactt ttagtgcggg cgctgattta agcagcgatg gcacacatgt
    17461 gatcacagca tggaccaatt atgccgatga tatgtttaac ggcaatgatt ccagtaatgt
    17521 atcaataaat aatttgttta cctatttaac cacaggattt cctgaaaaca tctgttatga
    17581 cggcggaacc atattcccct cccctcttgc aggcggcggc acatggagtg gtgaagccat
    17641 tattgatcca ttaaccggcg aaatggatcc aactttagtt ggtggaattg gcggaagcac
    17701 agatgtcaca tactccttta ccacaacagg tagctatacc gttacacaaa taccttatgc
    17761 tcctgcagtt cctgcatttg aaacaacact cggacttggt gatgatgcta ctgccaatga
    17821 tattaatatc ggatttccat ttacctattt cggcaatact tacacgaaat tatttatctc
    17881 gtcaaacgga ctaattggtt ttagtgcacc atcaacttct tatacccaac aacattttcc
    17941 taatgcagcc gatcctgata atattattgc attttgctgg acagatttaa acccatctgc
    18001 cggcggatca atttcatatg aaacaattgg tgttgctccg aacagaaaat tcatcgttta
    18061 ttatgacgat gttgtgcatt accttggcac agccactgtt acaggtcaga ttgttttaca
    18121 cgaaacatca aatgcaatcg atatcatcgc ggtaaatatt gaaagtgatg gcggtaatat
    18181 gacgcaaggc attgaaaaca caaatggaac tgaagcatat aatgccggtg aagcttataa
    18241 taatgcagtc tggacaatgt ccgaaatgtc ttggcgatat gctgttacac cttgcgatgg
    18301 ggaggttact gaaacaatca attttgtaac accacctgta gttgaattac ccgatagtgc
    18361 tgtttgccag ggctcgtccg taacgctgga cgccggcacc ggagcggagt attatgtatg
    18421 gagtacaggg gaaaatacac aatccattac tgttgatgaa acggatgatt attgggtgac
    18481 atattatgcc aatgaatttt gctttgtaac tgattcagca agtattattg taaacagtaa
    18541 tcctcctgta gatcttggtc ccgatggaac aaaatgcgaa ggcaccatgc ttgatgcaga
    18601 aaatcccggt gctgattatt tatggaatac cggagctacc acgcaaacgc tttttgtcac
    18661 tgagcccggc acctattcag tgtatgtaga aaatcctatc accggatgtt ataacagcga
    18721 tacaatcacc atgagtataa ttccattgcc tattgcagat tactctgcag tagcaactga
    18781 ccttaccgta gttttcacaa gtcttgccgg cgatgttgac acatggttct gggattttgg
    18841 tgatggaagt acttcaattg aagaaaaccc ttggcatata tatccgtttg cgggtgatta
    18901 taccgttact tttgttgtta cgaatggttg cggtgcagat ctcatatctg aggtgttcag
    18961 tattacaacc ggtataaatg ccttaagtca aaatgcatta tcaatttatc ctaatccggc
    19021 ttctacaacc attcacgtaa gcggattacc caatggtcaa gagcaacatt tcagaattat
    19081 gaatatgaca ggaagcgtag tgcttgaagg aaagtcaatg catgatatcg acattagtgc
    19141 cttacctgca tcaacttatg tgttgctagt ggatgatgga tccgggcaat ttaagggtac
    19201 ctttgtgaaa gactaattgc ttaccggaag cagcagattg tagttaatgt tgatattgca
    19261 ttgtttagat aaagctcatc gcatatccta ctaatgcacc gcctaatgtt ccaagcagga
    19321 tgtagatgat agcatgttta gcatattgtc gcaatccttt tctcactgct tttgccacca
    19381 aaccggcaac catggcgatg tttttttctg ctgtgatacg gttcatcaat aatttaggga
    19441 gatgcgattc catttccaat ttaaaaatgg ttcgtatttc cgcaattaat ttttcatcca
    19501 tgaacatact cagcaccggc attttttttg tgagttgatc cgacataaaa tgatcaaaca
    19561 aatcgagaat atctttttcg atatgtttga gatttttggt gtcaaatttc tcgggaatct
    19621 gttgtaggat atcagcaacg actgcggcaa agcccttatg caattttaca cccagcaatt
    19681 ttttatgtcg aagggtgtgt tgccataaat agatattcac acatccacca acaaagccgg
    19741 cagcggcacc ggaaacgata tgtgcagtta ctgcatccat gtagaagggc tttcgcgcca
    19801 ggtattaagc atgtctgctg tatcggcggg aattatttga tttttcacgg catgcgtaat
    19861 taaagaagga taatctgaca gtgtaaacaa tttgcaggaa gcagcattaa aagcctcatc
    19921 cgctaccgga aatgcatagg tgaagagagc agccattcca agcacctgcg cttcgacatt
    19981 gcgaagggct gcaactgctg ttaaactact tcccccggtg ctcaccaggt cttcaatcac
    20041 cactaccctg cttcccggtg cacatcggcc ttctatttga tttgatagtc cgtgcttttt
    20101 agcttcggag cgcacataaa tcataggtaa acccatggca tctgcaacca acgctgcatg
    20161 tggaattccg gcggtagcca cccctgctat cacttcaacg ttaggaaatt gttgcctgat
    20221 accttttgct aacgtatttt tgatccaggt tctgacttcg ggatacgata aggtaagcct
    20281 gttgtcgcaa tagatcggtg agcgccatcc gctggcccag gtgaagggtt catttggacg
    20341 cagtttgatg gcctcgatag caagtaattt ttccgcgaaa acaatcgcat catcagattg
    20401 aaagagcatg cctcaaagct ataaaatttt catcaacgaa aatcctgttt tcctcattgc
    20461 aggatcaatt cctgtaaatg ccaacctcaa cgacagcgcg catccgatat tgaattttaa
    20521 tcaggaaaaa gaggttgaaa aagcttttca cctgctcgag aatcatgccg gaattaaaag
    20581 cctgactatt tatggtgaca acgcaaaaga aatgcgaaac accttgtttg caggttataa
    20641 aaaaattgca gccgcaggtg gattggtctt taataataaa ggacaggtat tattgattta
    20701 tcgtcgcaac agttgggatt tgcctaaggg caaaatagat gccggcgaaa agaaaaaggc
    20761 cgctgcttta cgcgaagtgc gcgaggaaac cggactagag aaactatcca tcatccgcaa
    20821 actaaaaaag acttatcaca cctatttact ggaaaataat acacctgttt taaaggttac
    20881 acattggtat ttaatgatgt gcaatgatgt aacgccgccg gttccgcaaa ctgaagaaga
    20941 tatcgaatta gcaacatgga tcgatatcga tgaggtggaa ttaaagttca acaaaaccta
    21001 cgctaatatt caagacgtga tacatagtgg tgtaacgatg atgcactacg gttaattacc
    21061 cgaaattaca atttgtggca ccatagaact gatatcgcca ccgtttttat acacttccct
    21121 tacgatactc gaattaatgt gcgcatgggc cggatcgctc attaaaaagt aggtttcaat
    21181 ttccggcgcc aatgatttgt ttgtttgagc aatagccaac tcatagttgt aatccgcggt
    21241 ggaccttaag ccgcgaagca ggaattgagc gtgattgtgc cgacaaaaat cgattgtgag
    21301 tccttcataa gcatgaatct gcactttttt ctcgtgcaga aatatttcct gcagccagtt
    21361 tacacgttgc tcgagactaa aaaatccgct ttttgtgctg ttgtgcccga tgccaatgat
    21421 aatctgatca aatagcggca aggcgcgttc tataatattg atatgcccta aggtaacagg
    21481 gtcaaatgaa ccgggaaaaa cggcaatctt ggtcataaca aatacaatgt tttaaacggc
    21541 atctccacgc ttcacaactt ccgcctgaaa gatatgaaat atggtagttc catagttgcg
    21601 actacgcaaa aaatgtggat gatctgtaaa tgaatattta ggatttgttt ctaaaacgaa
    21661 ccatccctca ggatttaata attgattggc aaaaacctct tcaggcagtt ccgggatatt
    21721 aggtagcgga tatggcgggc ctgcaaatat caaatcgaat ttctttttgg ttgtgcgcat
    21781 gtattcaaac acatccattc gaagcacatt gatggtaatg ttcagttccg ttgatgtttt
    21841 gcgaatgaaa tcgacactgc gattatccat ttccacgcct acgatatccg tgcaaccgcg
    21901 cgaagccatt tcataacaca ggctacccgt tccacagaac agatccagat aggcaacatc
    21961 ttcgaaatta aaattattgg tgaggatatt gaataatccc tctttcgcaa aatctgttgt
    22021 aggcctggcc ggaatattgg ctggcggatg aaatcttctt ccacgaaatt ttccggcaat
    22081 tattcgcata ggtctaagct gaagagtgaa ttaaacatat gcaccggttt ttctctcacg
    22141 gcatccggta aaataatgtg ttcattcggc tgcaagaaag aaatattgct gaagaatttc
    22201 cataattcat tatacaacat ggaatcaggt aatatttcgc ccgacagttt caaatcacag
    22261 tgttcgcgat ctatttcaaa ctgatcggcc attaataata cgtaatacaa gaaatcatcc
    22321 ttgttcacat acttaaattg gttcatgaac acgaattcct ttttggcata atacatcaga
    22381 tacattacac ccggttttat gtcaaggatg atctgctgat cggcaaactg gtcgcgattg
    22441 cgaatagcag tttccatcaa tgcagtactg gcatgaaacc actttggatg cgtaaaaact
    22501 tcgtctaaat aggaggaaat tgcaagcggc aagctgaaca cattgcgtac gaatattcct
    22561 cttacctgat ttactaaaac agtagattgg tcatcgatgg gagaagtgag cgacaagtat
    22621 gcctttgctt tttcctcttc aaagattgtt cttggtaata aggtgaaagc actgtctaca
    22681 acaccaattt tcacctcctt atacattgcc tgaagggcat cttctgcagt gacactcact
    22741 ttaaattttg aaaacgcctc cggtgcgaat gctgaattaa atgctactgt tttcaataac
    22801 tggagcttac cgctgcgttt atcgtaaaca gcataggtgt attgatgctc ttccaattga
    22861 aataacagtg agtaatgact cgtaaaatcc ttgttaaaat ccgtgtcaac aaaatgcacc
    22921 ataagcgggt aaaattactg tttttttacc gggtgtcgtt ttatcgcctg tatgatgata
    22981 cctttgcaaa aaatctcaag atgtatagcc gtccgcgcag actccgttca aatgccatca
    23041 tgcgtgaaat ggtggccgaa acacgctttt cggtgcagtc gttgatgtat ccgatgtttg
    23101 tcacacatgg caaaggcatc aaccacccga taggtgcaat gccgggcatc agtcactttt
    23161 cgcccgatac tctggtaaaa gaagtggagc acttaatgag tttgggtatc aataagatac
    23221 tgttattcgg tgtgggggaa gacaaagatc cagttggcag cgcagcttac gactttacca
    23281 acgccgtagc gaatggcgtg cggtcattga aggcggcatt cggaaatgat ctgctcattg
    23341 ccacagacgt atgcatgtgc gcatataccg atcacggaca ctgtgggata cttcacggac
    23401 atgaggttgc aaacgatgaa acattgacat cgcttgcaaa aatggctttg atgcatgcac
    23461 aagcaggcgc tgatattgtt gcgccaagcg atatgatgga tggcagagtt gctgccattc
    23521 gagatacact tgatgccgaa aacatgcaac aggtggctat tatgagctat tcagtcaagt
    23581 ttgcttccgg ttattatgga ccttttcgcg aagcagccga ttcagcccca tctttcggcg
    23641 atcgcaagac ctatcaaatg gatgtaagaa atagcagaga ggctatcaag gaagctttat
    23701 tggatgtaga ggaaggtgcc gatattctta tggtaaaacc ggctctggcc tatatggatc
    23761 tgattcgcac tgtgaaagac caaaccttgc tcccgctggc atgttataat gtttcaggag
    23821 aatacagcat ggtaaaagcc gcagcagaaa agggctggat tgatgaacaa cggatagtaa
    23881 tggaaaatat ggcttccttt actcgtgccg gagctgatat cattattact tatcacgcaa
    23941 aggacatgct gtccaaaggc tgggtgtaat ccacaattcc tatgtatagt tcgtcttctc
    24001 cgccgatgga aaagcctgaa gattagtgca tctttacctc cgcattttac ggattgcaaa
    24061 ttaatatcac ataccgagaa attatacgcc tgagtatccc cctcatggtg agcagcctgt
    24121 tcaacaacct gatcagcctg attggcgttg catttatggg gcgagtaggc gaaactgagc
    24181 ttgcagcttg tggtgtagct gctttgatct acatcaccat aaccatgata ccctatgggt
    24241 tgtcggttgg gtttcaaatc attattgcca ggcgggcggg cgaaaacaat cccgagtcta
    24301 tcggtcgcat tttcaacaat aatctgatat tcctgatggg tgtggcggtg cttttatacg
    24361 cattcctgca gttcatggcg cccatcttgc ttaagccgat gttcagttct ccggaggttt
    24421 atgcggcagg catgacttat ctgaaataca ggaatattga gatatttttt gctgtatttg
    24481 ctttcacctt gctggggttt tataccagcg taggacaaaa tcaaatcatc tcttacagtg
    24541 cgattttaat gttggtggtg aatgttttct tcagctacaa cctggttttt ggcggaatgg
    24601 gcttgccgga aatgggtata ggtggagcag gacttgccaa cctcatttcg gcctctgcgg
    24661 catgtttgct caatttcggt tacttgtttg tgagtccttt aagagcgcgg tttggtttgt
    24721 tcaaattcga taaattcgac tggcccattg ttaagaaggg gcttgaactc agcggtcctg
    24781 ttgtacttca gcacatgctc tctacgggca cctgggtaat atttttcctc atgatagaaa
    24841 aaatgggacc tcctgccctg gcggcatcca atgttgcgaa ggaggtttat atggtgttgg
    24901 gtgtaaccac ctggggtgtt gccaatgcca caaatagcct tgtgagtaac cttctgggac
    24961 aaaaacgaca tgatgagatg ttcgatctca ttaaaaaggt cgtattgatt gcgctcgctt
    25021 tctcctttgc attctttctg tttattattc ttttcccgca ggttttcatc ggtattttca
    25081 ccaacgaccc aaccattact gctctggcaa cgggccctgt gcgcattgta ggcacctgtc
    25141 tggtaatgat ggctgtttca tctgtattgt ttagagccgt aacaggcacc ggagctacaa
    25201 gatattcctt gttgctggag ctgatcacgc tgatcgtata tatggtttat gtgatcatta
    25261 ttatcaaagt attggatgtc aatctcacca ttgcctggac aagcgagttt gtgtattggc
    25321 ttgtattggc ctggttatgc tggacgtatc tcaaaagtgg tgcatggaag cgccacaggg
    25381 tttaatccaa atgttaaact catcaccgtt tcaagacaat accttactgt atattgtatg
    25441 tatatacata tttcctacct ttgactcact ctccacgctg cacagcgcag ttcataatta
    25501 ataatttata aattaatgaa aaagaatgca tttgtatggg ttttcggact cattactatt
    25561 agcgtggtag gcacttccat gtcttatcaa aatcccaata acccaccact aggttacacg
    25621 ggagcgcccg gagaacagac ttgtcagaag gcaagttgtc atggtggggg caactttgta
    25681 gggacgccaa ccttaagtgg tttacctgat tctattgaac cgggagcaag ttattcatta
    25741 acgctcacat tggagagtaa ttgcaccaaa gcgggctttg agctcacctg tttggatgcc
    25801 gatgatgtga aagcaggaac gttttcggca ggaaccggga attccgtagc gaatttcggt
    25861 ggtaaacaat atgttcgcca gagttcacca aaaacgctta cgggcggaag cacatcctgg
    25921 accttcactt ggcatgctcc tgaaacagcg ggtggtgatg cagttacctt ctattttgct
    25981 tcattaaatg caaacggcaa tggtaacgat agcggtgata aagcaatcaa gggtgatacc
    26041 accattgcat ataaagttcc tgaagtgatt ctggatgcca ccgagattgc taaacctgaa
    26101 atcacagtca cgccaaatcc tgctaccgac catatcagca tcaaccatgc ggctgttggg
    26161 tctactatgc aattgtttga tatacacgga aagctgatca tgtctaagca tatcaacgca
    26221 gtttctgtaa cctattcaac tgctgaactg cctgccggga attacctgct tgcagttagc
    26281 aacaacggcg aacgaaccag caaacaaatt tcaattcaat aacttaactt attgtactat
    26341 aatgatttac atagattaca ttctatagtc acgtagataa agttggttaa cattaccggg
    26401 taacgctcaa gtttagtttc gagtgttacc cggttttttg ttttggtgag tggtgagtgg
    26461 tgagtggtga gtggtgagtg gtgagtggtg agtggtgagt ggtgannnnn nnnnnnnnnn
    26521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26581 nnnnnnnnnn nnnnnnnnnn nnnnngtggg cactatgcaa attcgctcgc tttggtattg
    26641 ttcatttaac tgtgtcatta tttgagttta atagtctatc tccaaaatag gtatgtaggt
    26701 ctaggcggac tttgttccag ctttgcgagc gaatggttgc attagtctca ctactcaata
    26761 cccactactc actatcggtc gtgaatcgtg agtcgtgaat cgtgactcgt gaatttttac
    26821 ctgatacttg atacctgata cctgatactt gatacctgat actaaatgag tatttcgaag
    26881 tgataaaaat acatctgtgt atctttgtta catgcggata ggcttatttt ttgggtcttt
    26941 taatcctgtg ca
//
LOCUS       DAOQHY010000018        26920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_784_length_26920_cov_6.620845, whole genome shotgun sequence.
ACCESSION   DAOQHY010000018 DAOQHY010000000
VERSION     DAOQHY010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 26920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26920
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_784_length_26920_cov_6.620845"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PLL28_02760"
     CDS             complement(<1..285)
                     /locus_tag="PLL28_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNF68264.1"
                     /translation="MEPDYISINRKAWNDKVDAHVQSEFYNVAGFLNGETSLKHIELE
                     LLGDISGKRILHLQCHFGQDTISLARMGAYVTGVDLSDVAIAKAKELAVET"
     gene            complement(433..687)
                     /locus_tag="PLL28_02765"
     CDS             complement(433..687)
                     /locus_tag="PLL28_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68265.1"
                     /translation="MDQQLPKHDCRNFVKKVFLVLDGELPEEERNLFIADIQRCKGCL
                     EHYHIEKTFKDFLSVKVERKECSNKLRDEIRNMVNNNPEA"
     gene            complement(690..1313)
                     /locus_tag="PLL28_02770"
     CDS             complement(690..1313)
                     /locus_tag="PLL28_02770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNF68266.1"
                     /translation="MSELATKSKRANFKPVENGNLTKSAKDRIFSKEFLPHMDALYNF
                     AFHLTLDEDDANDLVQETFLKAYRFIASYQQGTNAKAWLFKILKNGFINDYRRKTKQP
                     SKVDYDEVVSYHDTDDEGHVEYLDLRQELFQGMMGDEVTKALSELPVDFKTIILLCDI
                     EEFSYEEIARIIDIPIGTVRSRLHRARNILKEKLREYAQKEGFKEKR"
     gene            1413..2660
                     /locus_tag="PLL28_02775"
     CDS             1413..2660
                     /locus_tag="PLL28_02775"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNF68267.1"
                     /translation="MRSLLRVFPNQFFASLAILGLIVGLVTSKVVLSVATITLMCNAF
                     INIRAGSNFRKWFSDTTAMLCAALFLVYLFSGFWSSDIGYWVDRCRMKLPFLAIPLGF
                     TAVKAMSERAFHRWLGIYVVVIFGTSLVVLINYLMHYETLNHDLTMGQPIPAPLGDHI
                     RFSLELAFAIICGGYLWWKRFYFFSPKEHHWWGIITIFLAVCIHVFAVRSGISALYLA
                     LLIVILHSAFVRKKYLAAGIGIVSLFVIGYCSATYIPSLKSRMDYFRYEITLIRAGDI
                     KVDHSDAQRLLSMEYGLQVARQNIFFGVGAGDIKTEMNKLYEQHAQSDLVKSKMPHNM
                     YIYFLAATGMLGLLVFLLSVLYPWLSNKRYKNLLFSVFLVMMLFAFMAEHTLEIQLGT
                     AYYLLFLLLIKKHIDDSNTPAYA"
     gene            2653..3411
                     /locus_tag="PLL28_02780"
     CDS             2653..3411
                     /locus_tag="PLL28_02780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNF68268.1"
                     /translation="MHKITALIPTFNESHNIDAVIRSVQWCDEILVVDSFSTDDTVER
                     AKRLGARIIQSEYINSASQKNRNIPNASHQWILLVDADERVTGELRNEITALLKKSEI
                     PCDAYWISRRNFFMGKEIKYSGWQGDKVIRFFNRDTCRYEEKSVHAEVVTSGKIGHLK
                     SKLDHHTYKDVFHYMEKWDRYTTWSAQDAMKKNVNPGFYHFVIKPAFRFIKHYFLQLG
                     ILDGKAGYIISSLSAKAVFMRYVKVEGMRKMQSK"
     gene            complement(3422..4696)
                     /locus_tag="PLL28_02785"
     CDS             complement(3422..4696)
                     /locus_tag="PLL28_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68269.1"
                     /translation="MRVFLFALSILCVTHFTAAQDYCSMDRFDTYLWAESDIEVFTNL
                     EYGQAVNNSGNNEHLFLDLYHPPVAEDPLTYKPCVFFVHGGGLVGGDKDSEGAKDLGY
                     LYARMGYVFVSIDYRVGWNNGDADDGCGGDSTDLRRATYRAVQDTRAAYRWIKAYGPL
                     YGVDTNNIIVEGNSAGSMLLLYALYADQEDYDPMFYDELGSIDSAVNELTNFGFDPIG
                     CITEAAGIEDTTLLLIKDVPHLFFHGTCDSIVPFFHGPTFFCFQPFPYPTMNGSWPIV
                     QMFKRNDRNYHFYIAEGAGHDAVIPDSIIVYSAPFIKEILCNASTTKEFYRVLGKFKC
                     AVANEGELVITDVYPNPVVNTIYLEVTSTRQREITIDVYNNLGQSVLSGELPFTPPIG
                     EYTIDLSHLTRGIYYMRLSQRQEEYVLKFVKY"
     gene            complement(4794..6392)
                     /gene="nadB"
                     /locus_tag="PLL28_02790"
     CDS             complement(4794..6392)
                     /gene="nadB"
                     /locus_tag="PLL28_02790"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNF68270.1"
                     /translation="MHKVDFLVIGSGIAGLTFALKTARKFPSRTIAIITKAEEDETNT
                     KYAQGGIAAVWDFEKDSYEQHINDTLIAGDGLCKRDIVEIVVKEGPERVREIINWGAD
                     FDKTKEGEYALGKEGGHSQFRVLHHKDVTGKEIEKTLLEEAQSLPNITFFTHHFVIDI
                     ITQHHLGRIVTRHSPDIQCYGVYVLNKENKSIETVLSRITVMATGGAGQVYKSTTNPL
                     IATGDGMAMVYRAKGRVANMEFVQFHPTSLYQPGVSPSFLITEAIRGAGGVLRNSNGA
                     LFMQKYDSRESLAPRDIVARAIDNEMKLRGEDFVYLDCTPIAKDELLNHFPNIYEKCK
                     SIGLDILKDPIPVVPAAHYMCGGIQTDAFGHSSIQYLYSCGECSSTGLHGANRLASNS
                     LLEALVFANRIFIDACEKIDGIALADQIPDWNAEGTDDPKEMVMITQSVRELKEIMTN
                     YVGIVRSNERLDRALTRLHLLYEETEKLYETTTLSPQLCELRNLITVGFLITRGAAIR
                     KESRGLHYTTDYPHHSKWAEDSMF"
     gene            complement(6453..7022)
                     /locus_tag="PLL28_02795"
     CDS             complement(6453..7022)
                     /locus_tag="PLL28_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HNF68271.1"
                     /translation="MIIIQQTLVSDEILDEKFACNLQACKGACCVEGDEGAFVEPEER
                     KMLEKVYPKVKPYLTKEGIDGIEKQGLWVTNSEGVLKTPLMKDGPCVYTIWEGGIALC
                     GIEKAYLDGKVKWQKPISCHLYPIRLSKVGEYEALNYEEWDICKPACKNGKSLGIPVF
                     RFVKTAIIRRYGQEYYDALEATYAYRKGE"
     gene            complement(7111..7815)
                     /locus_tag="PLL28_02800"
     CDS             complement(7111..7815)
                     /locus_tag="PLL28_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNF68272.1"
                     /translation="MASLYLIPTILSEDALHTIPQYVHEITLRIRIFIVEDERTARRY
                     LRKTGYSVSFDDVTLLPLNVNTAPEEISGYMQYFSGDTEIGLMSEAGVPAVADPGSII
                     VNKCHTIGTRVVPLVGPSSILLALMASGMNGQRFAFHGYIPLKNPERKNQLQAMDIAA
                     KKGETQIFIETPYRNNQLIQEIISSCHGDTLLCIATDITGTHEKIKTLPVSAWKKNMP
                     QLDKTPTVFLLGTASK"
     gene            7860..8465
                     /locus_tag="PLL28_02805"
     CDS             7860..8465
                     /locus_tag="PLL28_02805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNF68273.1"
                     /translation="MNSTVLKKEFFTQTDTVGIARALLGKTLHTKFQGSHTSGIICET
                     EAYCGVEDRGCHAYNGRLTDRTRIMYAEGGVAYVYLCYGIHNLFNVVTHTTGEPHAVL
                     IRAIAPVGGINHMQKRRGLSDNTPQLAAGPGLVAQCLGLTTKHSGLSLFGEHIWITDA
                     PVLPDNDIIAGARVGMNFSGPYKTIPWRFRIKGSPNTSKAR"
     gene            8560..9318
                     /locus_tag="PLL28_02810"
     CDS             8560..9318
                     /locus_tag="PLL28_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNF68274.1"
                     /translation="MVFGLLLDFELRGAIVGFIIGCFMDTEYVFPEKKEKRDFRVSFM
                     MLGAFVMQTPGVGSKLSSNVVRSALVNTFGETYTQRRLSFFYELLRQRIQVEAICGQI
                     QQYASVGEKNTLLRFLFELGEQPGVNKENLHHSINYLAARIGVPFETVQQVYNTVKGK
                     MRDQQYSEEQTYEEQAHRPEQEFNSLSSSFTTLGLASTANEKEVKRAYYRLAKKFHPD
                     SNPQASADEKKLLESKLRLVIEAYERICEVKNWK"
     gene            complement(9456..10325)
                     /locus_tag="PLL28_02815"
     CDS             complement(9456..10325)
                     /locus_tag="PLL28_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNF68275.1"
                     /translation="MRQLKITKSITNRESESLEKYLQEIGKVDLVTAEEEVTLAQRIK
                     QGDQLALEKLVKANLRFVVSVAKQYQHNGLSLNDLINEGNVGLVKAAQKFDETKGFKF
                     ISYAVWWIRQSIMQALAEQSRLVRLPLNKVGSLSKINRAFSELEQKFEREPTPEELAE
                     ILDLAPDEIKNTLSVSSRHVSVDAPFDDGENSSLLDVLENRDVERTDEKLDYSHSLKV
                     ETERTLATLTERERDVIKLFFGIGVPYAMTLEDIGEEFGLTRERVRQIKDNAINKLRS
                     STRNKALKAYLAQ"
     gene            10657..11667
                     /locus_tag="PLL28_02820"
     CDS             10657..11667
                     /locus_tag="PLL28_02820"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68276.1"
                     /translation="MKNLQFQILMLLFATTALMQAQPTITSATAGGPGDEFTNKMIDA
                     ILFDPGASGADVTWDFSGVTLTGETNDYAFITAAATGQSAAFPDANVAADNGMGNYSF
                     YKINASVYENHGIYTPDVTVLYSDPEELMTFPITYNATNSDDFYSTIDAGVEIIRSGT
                     VETVADGYGTLKLPDGTYTDVMRVFVHEVYSDVTVGVPVDIDYDMYIWFWLKEGVVGP
                     LFTYYFSSVSTGGFPTISQVAMINESNSTVGIKDQTAQTTLKAFPNPANDMVHVSGVS
                     ASAKKIILTDLLGNNVSEIENPTISEGMISFSSATIPSGMYIAQVVDENSVQSVNIQI
                     LH"
     gene            complement(11752..12225)
                     /locus_tag="PLL28_02825"
     CDS             complement(11752..12225)
                     /locus_tag="PLL28_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNF68277.1"
                     /translation="MAQKITTFLTYNDQAEEAVEFYCSIFPDAKIVSKQYYSDSIPGL
                     GGKLMSATFTLFDQTYYALNAGPHFQFTEAISLFISCEDQAEVDRYWNALTEGGKEDR
                     CGWLKDKYGVSWQVIPKQLGMLLSHPDKAKASRAMQSMMGMRKIDIAELERASEG"
     gene            12338..13135
                     /locus_tag="PLL28_02830"
     CDS             12338..13135
                     /locus_tag="PLL28_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68278.1"
                     /translation="MKNLKLPIFAISIISIHACTTKPNVATPCNDIFETPKVIQHPTN
                     TPLENLLMDTMSFANYWIVEKYRFNDEIYYRKHPHTKYIKFKSDMVAESMLTQLLDTT
                     NNHGFQIITDYTTTLYDYYGDDLTPYACFPVFIVNPTQSTKVFVAKDSYVFAIQEAMD
                     PHNQQWYPIECLPYDFCGNGYYGIKVHPKEFVMIVAPKFQGDTKGMLRIRLKIGEDIY
                     VSQPYEGYFNSGQFVPPYFLQKRIDDPERLKYGIVRDFIGSVPKVVN"
     gene            13273..14187
                     /locus_tag="PLL28_02835"
     CDS             13273..14187
                     /locus_tag="PLL28_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF68279.1"
                     /translation="MSAPENCIVTENLTHRFGSNELVVQDVNLVIPRASIYGFLGPNG
                     AGKTTTLRLILGLLQKQQGKIYIKGMPLHKHRTLLMRSIGNLIESPSIYAHLSATENV
                     RIQQILFGTNRKRIQQVLQLVGLEKTGSKPAGKFSLGMKQRLGIAIALLHSPEILILD
                     EPTNGLDPNGMIEIRELLLRLHKENGMTILVSSHLLAEIEKLATHVGVIHNGILRFQG
                     STTELADLQRHQKGTLIATDNIRLSKQIFDEQGISYHIAENVFHVSVENIQRVVELNR
                     TLNERGVGVFKIEAQKHDIETIFMELIK"
     gene            14190..14957
                     /locus_tag="PLL28_02840"
     CDS             14190..14957
                     /locus_tag="PLL28_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF68280.1"
                     /translation="MMILQSIKSEWLKTKHSIATWLCLIGGTFIPLIFLIGMLKDHRT
                     IDMYQGELNAWTYYFFQVWQYMQVFLLPMGLILATSLITQVEYRNNTWKTVHTTPQSY
                     TAIFMAKFIVLLGMTGLFFLYFNATWYLSAAIPSLIFAGHFPPVDFPWNQFLTSNGKI
                     LLTCLPVLALQFLLSLHFKNFLVSIGIGLLLVVGSLILTGQWEYAYTIPYSYPALTMM
                     GDAAQLEKIHIYAWCLGYFIVFLTFGYVLYVTRKAKG"
     gene            14991..16055
                     /locus_tag="PLL28_02845"
     CDS             14991..16055
                     /locus_tag="PLL28_02845"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNF68281.1"
                     /translation="MKKYVVVLLNLGYWLVFMLLILVIFGLISSVSPTSDESDKPVLR
                     NWLGFMTSTTILPGVICFYLNYFFVFNRYLQKRKIPQFFIAVIVTALICGVVGAFSGA
                     APWLIGDEYYIANMSIGNLIAIVVFFGFVAMLNGVIGLVMRGFISWYGDIRIKESLAA
                     KNTEMELSLVKSQINPHFLFNTINNIDVLISKDPEKASEYLNRLSDIMRFMLYESKPD
                     RILLSREIAYITKYIDLHRIRSNNTDYVHFEVIGDPSEYMVPPMLFIPFIENAFKHVG
                     DKKKTPAINIKLIIENNSLLFNCCNHYFPDKIQDADTLNGGLGDKLIRRRLELLFPNA
                     HTLQLTTDNEQYCVSLKIIL"
     gene            16058..16738
                     /locus_tag="PLL28_02850"
     CDS             16058..16738
                     /locus_tag="PLL28_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNF68282.1"
                     /translation="MRCLIIEDEPLAQERIKQYIARLPFLEMVGSFDNAIDALLFLRE
                     HHIDLLFLDINLGEMSGIQLLEVIKPNCPVIITTAYQEYALKGFELNVTDYLMKPFTF
                     ERFYQAVEKARAGTQMVVTQEKAFIFVKTEHRLEKVMLDDIQYIEGMRDYRRVHLSDK
                     SIMTLQTFTEFEKILPPQKICRIHKSYMVSISKITSIEKDRVLIAGKRLPISETYKKS
                     FLDIIGAK"
     gene            complement(16800..17138)
                     /locus_tag="PLL28_02855"
     CDS             complement(16800..17138)
                     /locus_tag="PLL28_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HNF68283.1"
                     /translation="MKKILAIIGLFMAIGMQTSFAQNSGKQYIEIKTSAVCGTCKAAI
                     ETALEKVDGVKSADLDLETKVVSIKFDGDKTNADALKTAITEAGYTADEVPANKVAYD
                     NLHPCCKSEQ"
     gene            complement(17166..19346)
                     /locus_tag="PLL28_02860"
     CDS             complement(17166..19346)
                     /locus_tag="PLL28_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF68284.1"
                     /translation="MAAPVFAQQMVEGEVMTNDDKGKMIPLPGANVYWEGEYSGVISD
                     DEGHFSIEQKTTSQRYLVVSYVGFSADTFDVQTTKHIHVMLSPDTELKTVVVEGAQSS
                     KTINTVDPIGTEQLNKKELLKAACCNLSESFETNNTVDAEFSDAVTGAKTIKLLGLDG
                     VYAQIMTENMDNVRGLSSAYGLTFIPGPWIESIQITKGPGSVVNGYEGITGSINTEYK
                     KPFDADEETARLDVYGNQFGRWEANAVYKHPFNLKWSTGLYGNVSQMQQKVDHNDDGF
                     LDVPMNSTYTFMNKWNYFSGGMHEAQFGVKYINSNVTGGQTSFDVNSPRTTENGYGVG
                     ADISRWELFMKNGFVFKRPSTSIGTILQFADHNQTGFYGLNNYAGAEQFIKAEAIFVT
                     YIGNTNSTIKMGLNYLYDNFDESFDSITYLRNESVPGIFAEYHYQFEKKWSVLAGLRV
                     DQHNLYGTFVTPRMHVKFTPKENTTLRASAGKGYHIANVLIENSSILTSNRTLIIQEP
                     LEPEEGWNYGISLIQQFKLNHREGTFTLDVYRTDFINQVVIDMDANVNEINVYNLKGE
                     AYTNVLQAEINYEPIKRLELRAAYKYVDSKATYDGKLLEVPLTFKDRGLFNVAYNWKK
                     AGLSADATAQFYGKTRLPDLSENHAAHDFGTNSEPYIQLLAQVTKSFGDLDVYVGSEN
                     ITGYTQHNPIIDATDPFGENFDAGVIYAPLMPRMFYGGLRYIFN"
     gene            complement(19480..19875)
                     /locus_tag="PLL28_02865"
     CDS             complement(19480..19875)
                     /locus_tag="PLL28_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68285.1"
                     /translation="MKRLRAIFGMMMLVLLFGITSGFNLFTHLCLMTGESGVAPVSMS
                     MDCCGNQLPDAPVTLSMNCCIEKTQFIKFEYSGSVQNQVGYTDYADLDYPVIQIQDEI
                     QADVVNLNSFNLPPPFPGDIRVMQCIYRI"
     gene            20010..20813
                     /locus_tag="PLL28_02870"
     CDS             20010..20813
                     /locus_tag="PLL28_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68286.1"
                     /translation="MKRNFGSALLAICTLLTVSSCSKKEGCTDPTATNYDPDAEKNVG
                     CLYEETYAVEMHVHQFLNDIELEEEMPYVINGVNTNLNLAQFYVSGITLVDANGNEFK
                     QDGLYLLVQPETEEYAIGDFPAGDYTSVKFDIGIDSATNHGDPSLYPIGDPLGAQTPS
                     MHWGWSFGYIFMRIDGEVDSDGDGTPDPAGAFEMHLGTDHYLASIEVEMPISIGQGLE
                     NIVHLQADYTSFFTGVDMVNDNTTHTTDNVTLANLLLDNIYTMFSPGEE"
     gene            20828..21838
                     /locus_tag="PLL28_02875"
     CDS             20828..21838
                     /locus_tag="PLL28_02875"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNF68287.1"
                     /translation="MRKLISILFLSAILVSCSEVIPEEKAYELQIPPGFDVPQIPEDN
                     ILTDAKIALGKKLFYSTALSVDSTISCASCHRPENAFADHLAISHGVMGRTGFRNAPS
                     LQNVAWYTVITMDGGNPSLETQPYVPIETHEEMGFNMVLLCERLNTDAEMVTSFNDVF
                     GSNPDPYGVTRALAAFERTMISGNSRYDQYAYQGNIHALTAEELQGKDLFFSDALGCA
                     NCHSGFLFTNNTFENNGFFSDYSADSGRARITWLHEDVGKFKVPSLRNVGVTAPYMHN
                     GQVETLDEIIDMYAAGGSGHENQSVFITGFSITPAEKEALKAFLLSLTDISFMQNPDL
                     LE"
     gene            21869..22978
                     /locus_tag="PLL28_02880"
     CDS             21869..22978
                     /locus_tag="PLL28_02880"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNF68288.1"
                     /translation="MFRKHRIWILPIAFFATIMLFTKCDPDDPITPDDEWVYDDTPYT
                     ADHVNGIPPMPVFTTNPLTVKGVELGRMLFYDPILSADSTLSCSGCHSVAHAFSDTTA
                     FSIGIDGLPGKRNAMPIFNLGYAVAINATFFWDGRAATIEDQTLMPIQDPLEMHEILP
                     NVITKITRSEFYPKAFYEAFGEEVITTELVARALGQFLKSVTSGNSRYDQAMAGEIFL
                     TDEETLGFTLFNALDGADCFHCHGVNGGLFTDYVYRNNGLDDATHYTDFADAGLGAIS
                     GDTADYGKFKTPSLRNIELTAPYMHDGRFATLEEVLDFYSEGVHDTPFTDNLIQFAYQ
                     GGVQLTAEEKAAIIAFLKTLTDTNFETNEAYQNPF"
     gene            23209..23478
                     /locus_tag="PLL28_02885"
     CDS             23209..23478
                     /locus_tag="PLL28_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68289.1"
                     /translation="MFTDRNIDIFLAGLVTRVALSFPIAYIRSIALVSNNKKIADCNW
                     ESDPRGTVTRYDDLHVPVAAEQVVVEGLDTNGQPLMITAAIASLH"
     gene            24086..26095
                     /locus_tag="PLL28_02890"
     CDS             24086..26095
                     /locus_tag="PLL28_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HNF68290.1"
                     /translation="MDSAQSNFQRVLQFDRRHYSNDQLIQIYKRLLKPRMIEEKMLNL
                     LRQGQVSKWFSGIGQEAVAVAVPYVLEYDEYILPMHRNLGVFTGRDIPFAKLFSQWQG
                     KKNGFSKGRERSFHFGTNEYHIVGMISHLGPQLAVADGIALAAKLKNEKKVTAVFTGE
                     GATSEGDFHESLNVAAVWDLPVIFIVENNGYGLSTPTNEQFRCAQIADKAIGYGMRSM
                     IVDGNNILDMVAALEECRAYCINEQKPVLLECMTFRMRGHEEASGIKYVPKELIEMWG
                     KKDPVQNYEQYLLESAIINEEMIKTFRDEIKSEIEAGVQTVFNEPEVIPDTEEEERDI
                     YAPFTQEVIAPKSNIASQKKFINAISDALKLAMDKYPELIIMGQDIAEYGGAFKVTEG
                     FVQQYGKSRVRNTPICESAILGAGCGLSVKKMKSVIEMQFADFVTSGFNPIINNMAKM
                     YYRWGQNVDSVVRMPTGAGVGAGPFHSQSNEAWFFHTPGLKVVYPSSVYDAKGLLLAA
                     IEDPNPVMFFEHKALYRAISDNIPDDYYTVEIGKAALAQSGNELSIITYGMGVHWAKK
                     ACAQLQIDADILDLRTLLPLDYDAIATTVRKTGKVLVLHEDTLIGGIGGEIAAYISEQ
                     LFNVLDAPVMRCASLDTPVPFSLELEKNFLPESRLIEKLKALASY"
     gene            26136..26567
                     /locus_tag="PLL28_02895"
     CDS             26136..26567
                     /locus_tag="PLL28_02895"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HNF68291.1"
                     /translation="MSIHLQHLESELEENTRRRGYDFYCEGRVRSVFNINGIEWMAVV
                     QGYDDHVVELRITNDTVMHYACNCNDEEDPCKHVAAVLYYIRKESHKQKRNDPSGYDE
                     MKEEISYLPPHVLRYLILKYAAQSKDFRTFLGEYFNGDTRK"
     gene            26704..>26920
                     /locus_tag="PLL28_02900"
     CDS             26704..>26920
                     /locus_tag="PLL28_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68292.1"
                     /translation="MNNEQAIGASRKHIAELHPEVIDWINYSLFVKDEVKSLNHRLME
                     IAHRYTDHDVMAQVEHFQNQFIRQDEAS"
BASE COUNT         7805 a         5646 c         5520 g         7949 t
ORIGIN      
        1 tgtttctaca gccagctctt ttgcttttgc aatggctaca tcgctaaggt cgacgccggt
       61 gacatatgca cccatgcgcg ccagcgaaat tgtatcctga ccaaaatgac attgtaagtg
      121 taaaatacgc tttccggaaa tatcaccaag taattccagt tcaatatgtt tcagggaggt
      181 ttcgccattt agaaaaccgg caacattata aaattctgat tgcacatgag catcaacttt
      241 atcgttccat gccttgcgat taatagagat ataatccggt tccataggat attaatttaa
      301 ttggacgaaa atggtcgtca atggtgagta attgtaaagg ttaaaggtcg ctcatggcga
      361 tttaaccaac aataaaaatt accaacaaaa attttgattg ccttttggct tattgtaaag
      421 agaagattgt gatcaggctt cggggttatt atttaccata ttgcggatct catcgcgcag
      481 tttgttgctg cattcttttc tttccacttt tactgagaga aaatccttaa aagtcttttc
      541 tatatgatag tgttcaagac agcctttgca acgctgaata tcggcgataa agagattgcg
      601 ctcttcttca ggcagttctc cgtcaagcac taggaacacc ttttttacga agtttctaca
      661 atcgtgtttg ggtaattgtt ggtccataat caacgttttt ctttaaaccc ttccttttgg
      721 gcgtattcgc gtagtttttc ttttaaaata ttgcgtgccc gatgtaaacg cgaacgcaca
      781 gtaccaatgg gaatatcgat gatcctcgca atttcttcat atgagaactc ttcgatatcg
      841 caaagcagga taatggtttt aaagtcgacc ggtaattcac tcagggcctt cgttacctca
      901 tcgcccatca tgccctgaaa taattcctga cgcaaatcta aatactccac atgcccctca
      961 tcatctgtgt cgtggtaact caccacttca tcataatcta ccttcgaggg ttgcttcgtt
     1021 ttgcgacggt aatcgtttat gaacccgttt ttcaggatct tgaacaacca cgcttttgca
     1081 ttcgttcctt gctgatacga tgcaataaat cgatacgcct tcaaaaaagt ttcctgcacc
     1141 aggtcgtttg catcatcttc atccagcgta aggtggaaag cgaagttata cagcgcgtcc
     1201 atgtgcggaa ggaactcttt cgaaaaaata cggtctttcg cgcttttcgt taggttgcca
     1261 ttctccacgg gcttaaaatt agctcttttg ctctttgttg ccaattccga catgcacgcc
     1321 tttgggctta ttaagagaaa atatctacat ttggttccct attcacccaa acccgaccgc
     1381 taccgataac actgctacga atttttagca acatgcgatc attactcagg gtttttccca
     1441 atcagttttt tgcctcactg gcaattctcg gacttattgt cggcctagtc acttcaaaag
     1501 tggtcctcag tgtggcaact atcaccctca tgtgtaatgc ctttatcaat atcagggccg
     1561 gtagcaattt tcgtaaatgg ttctccgata ctactgccat gctttgtgct gccctcttcc
     1621 tggtgtacct attctcagga ttttggagca gcgatatcgg ctattgggtc gaccgctgcc
     1681 gcatgaaact cccgtttttg gccattccgc ttgggtttac tgccgtaaaa gctatgtcag
     1741 aaagggcttt tcaccgttgg ctgggcatct atgtcgtggt catttttggc acttcattgg
     1801 tggtgttgat caattatctg atgcattacg agacgctgaa tcacgatctc accatgggtc
     1861 agcccattcc tgctcccctg ggcgatcaca tccgttttag cctggaactg gcttttgcca
     1921 tcatttgtgg ggggtattta tggtggaaga ggttttactt tttttccccg aaagaacatc
     1981 actggtgggg catcattacc atttttttgg cggtgtgtat acatgtgttt gccgtgcggt
     2041 cagggatatc ggcactttat ctcgctttgc ttatcgtaat tctgcatagc gccttcgtca
     2101 gaaaaaaata tctggccgcc ggaataggaa ttgtctcctt gtttgttatt ggatactgct
     2161 ctgcaaccta tattccatct ctgaaatcaa ggatggatta tttccgatat gagatcaccc
     2221 tgatccgtgc aggcgatata aaagttgatc atagcgatgc acaacgatta ttaagcatgg
     2281 aatatggact ccaggttgcc aggcaaaata ttttctttgg tgtaggtgcc ggagatatta
     2341 aaactgagat gaataaacta tacgaacaac acgcccaaag cgatctcgta aaatctaaga
     2401 tgcctcataa tatgtatatc tattttttag cggctacagg catgcttgga ctgctggtct
     2461 ttcttttatc ggttttatat ccctggctaa gtaataaacg ctataaaaat ttactgtttt
     2521 ctgttttttt ggtgatgatg ctttttgctt tcatggcaga acatacttta gagattcaat
     2581 taggtactgc atattatctc ttatttttgt tgttgatcaa aaaacatatc gacgatagta
     2641 acacgcccgc ttatgcataa gatcactgca ttaataccaa catttaacga gtcgcataac
     2701 attgatgcgg tgattcgaag tgtacagtgg tgtgatgaaa tattggtagt tgactcattt
     2761 agtactgatg atactgtcga acgcgctaag cgtctcggcg cgcgcatcat acaaagtgaa
     2821 tatattaatt ccgccagtca gaaaaatcga aacattccca atgcctcgca tcaatggata
     2881 ttattagtag atgccgatga aagggtaacg ggcgaactac gtaatgagat aacagcactc
     2941 ctgaaaaaat ctgaaatacc ttgtgatgca tattggatca gcaggcgcaa tttttttatg
     3001 ggtaaggaaa ttaaatacag tggctggcag ggcgataaag ttatacgatt ctttaatcga
     3061 gatacgtgtc gatatgagga aaagtcagtt catgcagaag ttgttacttc cggaaaaatt
     3121 ggtcacttga aatcgaagct tgatcatcac acatacaaag atgttttcca ttacatggag
     3181 aaatgggatc gctacactac ctggagcgca caggacgcta tgaagaaaaa tgtcaatccg
     3241 ggattttatc acttcgtaat caagcctgct ttccgattta ttaaacatta ttttttgcaa
     3301 ctaggcattc ttgatggcaa ggccggatac atcatttcat cactaagtgc gaaagctgtt
     3361 tttatgcgat atgtgaaagt ggagggaatg agaaagatgc aaagtaagtg atgaagcgcg
     3421 cttaatattt cacaaatttc aaaacatatt cttcttgtcg ctggctgagg cgcatataat
     3481 aaatacctct tgtcaaatgc gataggtcta ttgtgtattc accgattggt ggcgtaaagg
     3541 gaagttctcc cgaaagcacc gattgtccaa gattattata aacgtcgatg gtaatttcgc
     3601 gttgccgagt gctcgtaacc tcaagatata tggtattaac caccggattt gggtacacat
     3661 ccgtgataac taattccccc tcattggcca ccgcgcattt gaatttgccc aacacgcgat
     3721 aaaattcttt tgtcgtagac gcgttgcata atatctcctt aatgaatgga gccgaataaa
     3781 caatgatgga atcaggaatt accgcatcat gaccggcgcc ttcagcaata taaaagtgat
     3841 agttacggtc attgcgttta aacatttgca caattggcca tgagccattc atggttggat
     3901 atggaaatgg ttgaaaacaa aaaaatgttg gcccatgaaa aaatggaact atagaatcgc
     3961 aggtgccgtg aaaaaataaa tgtgggacat cttttatcaa cagtagtgtt gtatcctcaa
     4021 tacctgcggc ttctgtaatg cacccgattg gatcaaatcc gaagttagtt aactcattta
     4081 ctgctgaatc tatacttccc aattcgtcgt aaaacattgg gtcataatct tcctggtcgg
     4141 catacagtgc gtataataat aacattgaac cggcactatt tccttcaaca ataatgttat
     4201 tggtatctac accatataat ggtccgtagg ccttgatcca tcggtatgcc gcccttgtgt
     4261 cttgcactgc tcggtaagtt gcccgcctta gatctgtcga atcaccgccg caaccatcat
     4321 ctgcatcgcc attattccat cccacacgat agtcgatact cacaaaaaca tagcccattc
     4381 gtgcatacaa ataacccaga tctttagcac cttcgctgtc cttatctccc ccaactaaac
     4441 caccaccatg aacaaaaaaa acacaaggct tatatgttaa tggatcttct gcaacaggtg
     4501 gatgatataa gtccagaaac aaatgctcgt tgtttccgga gttatttaca gcttgcccat
     4561 attcaagatt tgtaaatact tctatatcag attctgccca caaataggta tcgaaacgat
     4621 ccatcgaaca gtaatcttgc gcagcagtaa aatgcgtaac acacaaaatc gacaatgcaa
     4681 atagaaacac acgcatcatg caaaggtagc aaatgcctat gctatcaata caaattttac
     4741 gccaaacggc ctgtaaatgt taaaaaaacg acattaaaat tactcccgca tctttaaaac
     4801 atactgtctt cagcccattt gctgtgatga gggtagtcgg tggtgtaatg caatccgcgc
     4861 gactcttttc tgatggcggc gccgcgtgtt atcaggaaac ccaccgtgat taaattcctt
     4921 agctcacaga gttgcggaga gagtgtagtt gtctcgtata atttttctgt ttcttcgtaa
     4981 agcagatgca agcgagtcaa tgcacggtct aagcgctcgt tactgcgcac aatgccaaca
     5041 taattggtca tgatttcttt caattctcgc acactttgtg tgatcattac catctccttc
     5101 ggatcatctg ttccttcggc attccaatcc ggaatttgat cggcaagcgc aatgccatct
     5161 attttttcgc atgcatcaat aaaaatgcga tttgcaaaaa ccaatgcttc caacaacgaa
     5221 ttacttgcca gtcggttagc gccatgtaat ccggtcgacg aacactcacc acaggaatat
     5281 aaatattgaa ttgatgaatg tccgaatgca tccgtttgta taccaccaca catataatgc
     5341 gcagcgggaa caacagggat aggatctttc aaaatgtcaa gccctatcga tttacatttt
     5401 tcgtaaatat tcgggaagtg attgagcaat tcatcttttg ctatcggtgt gcaatcgaga
     5461 taaacaaaat cctcgccgcg caatttcatt tcattatcga tggcacgagc aacaatatcg
     5521 cgaggcgcta atgattcacg agaatcatat ttctgcataa acaaagcacc attgctattg
     5581 cgcaaaacac ccccggcacc tctgatagct tcagtaatta aaaaactcgg agatactccc
     5641 ggttgatata atgatgtggg atgaaattgc acaaactcca tattcgcaac ccgacctttg
     5701 gcgcgataca ccattgccat accgtcgccc gtagcaataa gcggattagt tgtgctttta
     5761 tacacctgtc ccgcaccacc cgttgccatt actgtgatac gcgataatac cgtttctatg
     5821 gatttatttt ccttgttgag cacatatact ccgtaacatt gaatatccgg cgaatgtcgt
     5881 gtaacaattc ttcccagatg atgttgcgta ataatatcaa taacgaaatg atgtgtaaaa
     5941 aaagtaatat ttggaagcga ttgtgcttct tccaacaatg ttttttctat ttcttttccg
     6001 gtaacatctt tgtgatgtaa aacccggaac tgcgaatgac caccttcttt gccaagcgca
     6061 tattcgcctt ctttagtttt atcgaaatcg gcaccccaat taattatttc acgtacgcgt
     6121 tcaggtcctt cttttaccac tatttcaacg atatcgcgct tacataatcc gtcacccgca
     6181 atcaaggtgt cgttaatgtg ttgctcgtaa ctgtcttttt caaaatccca tactgcggca
     6241 ataccaccct gagcatattt tgtatttgtt tcgtcctctt cagctttagt gataatggca
     6301 attgtgcgag aaggaaattt tcgcgccgtt tttaatgcaa acgttaatcc cgcaattccc
     6361 gatccgatga caagaaagtc gaccttatgc atataacaaa ggtaataagg tctgatgaaa
     6421 gcaatactta aatggtgatg gccgcgagga cactattcgc cttttctgta ggcataagta
     6481 gcctccaacg catcataata ttcctgcccg tagcgacgga tgatggcagt tttaacaaat
     6541 ctgaacaccg gtatgcccag cgacttgcca tttttacatg ccggtttgca aatatcccat
     6601 tcttcgtagt tcaaggcttc atattcacct actttggaca atcggattgg gtagaggtga
     6661 caactaatcg gtttttgcca tttcaccttt ccgtcgagat aagctttttc aattccacag
     6721 agtgcaattc caccttccca gatcgtgtat acgcaaggac cgtctttcat caaaggggtt
     6781 tttagaacac cctcgctgtt ggttacccaa agcccttgtt tttcaattcc gtctatacct
     6841 tcttttgtga gatagggttt taccttggga taaacctttt ccagcatctt gcgctcttcc
     6901 ggctctacaa aagcaccctc atcaccttca acacaacagg cgcctttgca tgcttgcagg
     6961 ttgcatgcaa atttttcgtc aagaatttcg tcgcttacaa gagtttgttg aatgatgatc
     7021 atactgcaaa aatacaatta ttcctcagtg aaaatattgt caattgtgaa ccgatttgag
     7081 ccaaaacatc acttccatac aaggagcatt tcacttggaa gcagtgccta ataaaaatac
     7141 ggttggcgtt ttgtctaatt gaggcatgtt ttttttccat gccgaaacgg gtagtgtttt
     7201 aattttttcg tgagttccgg taatatctgt cgctatgcat aataatgtat cgccatgaca
     7261 tgaagaaata atttcctgta tcaattgatt gtttctgtac ggcgtttcaa taaatatttg
     7321 tgtttcgcct tttttcgcag caatgtccat cgcttgcaat tgattttttc gttccggatt
     7381 ttttaacgga atatatccgt gaaacgcaaa ccgctgacca ttcattccgc ttgccattaa
     7441 tgccaacaaa atactggatg gtccaaccag tggaacaacg cgtgtaccaa tggtatgaca
     7501 tttattcaca ataatacttc cggggtcggc aactgcaggc acaccggcct cgctcattaa
     7561 tcctatttct gtatctcctg aaaaatattg catatacccg gaaatctcct ccggagctgt
     7621 gttgacattc aaaggcaata aggtaacatc atcaaacgat accgaataac ctgttttgcg
     7681 caaatagcgc ctcgcagtgc gttcgtcttc aacaataaaa atgcggatgc gcaatgtaat
     7741 ttcatgcaca tactgaggta tggtatgcaa tgcatcttca ctgagtatgg ttggaattaa
     7801 atataatgag gccatttgtt ttatcaggtg tatttttgtc tgcggctcaa caaatttgca
     7861 tgaatagtac cgtattaaaa aaagaattct tcacacagac cgatacggta ggtattgcac
     7921 gtgctctttt gggtaaaaca ctacacacta aattccaggg ctcacataca tccggcatca
     7981 tttgcgagac agaagcatat tgtggggtag aagatcgggg ctgccatgca tacaatggcc
     8041 gactcaccga cagaacgcgc atcatgtatg ccgaaggagg agtggcttat gtatatttat
     8101 gctatggcat tcacaacctg tttaatgtgg taacgcatac taccggagag ccgcatgcag
     8161 tgttgattcg agccattgca ccggttgggg gtatcaacca tatgcagaag cgaagggggc
     8221 tatccgataa cacaccacaa ctggctgcag gacccggctt agttgcgcaa tgcctcggat
     8281 taacaacaaa gcatagcggg ctgtcgttat ttggtgaaca catttggata actgatgcac
     8341 ctgtattacc cgacaacgac attattgccg gtgcccgtgt gggtatgaat ttcagtggtc
     8401 cgtacaaaac cattccatgg cgtttcagaa taaaaggatc gccgaacacg agtaaagcac
     8461 gataatttga aattcaataa aaacctcaga atcacctttc acacgcaaag cggagtcaag
     8521 atgactttga aggttttcgg tttgaataaa ttcgcgttga tggtattcgg gttactgctc
     8581 gattttgaac ttcgaggagc catagtcgga tttattatcg gatgttttat ggatacggaa
     8641 tatgtctttc ctgagaaaaa ggaaaaacgt gatttccgcg tgagctttat gatgctggga
     8701 gcctttgtta tgcaaacacc cggcgtagga agtaaattgt caagtaacgt ggtgcgcagc
     8761 gctttggtaa atacttttgg tgaaacatat acgcagcgaa gactgagttt tttttatgaa
     8821 ttattaagac agcgtattca agtggaagca atttgcggac aaatacaaca atatgcttct
     8881 gtaggagaaa aaaatacttt actaagattt ttattcgaac ttggagaaca acccggtgtc
     8941 aacaaagaga atctgcatca cagcattaat tatctcgccg caagaattgg tgttccattt
     9001 gaaaccgtgc agcaggtgta caacacagta aagggaaaaa tgcgcgatca acaatattct
     9061 gaagaacaaa cttatgaaga acaggctcac cgtccggagc aagaattcaa ttcattatca
     9121 tcttcattca caacattggg gctcgcttct acagcaaatg aaaaggaagt caaacgcgcc
     9181 tattatcgcc ttgcaaaaaa attccatcct gattcaaatc cgcaagcttc tgctgacgaa
     9241 aagaaattat tggaatcaaa attaagactg gtgattgagg cgtatgaaag aatttgtgaa
     9301 gtaaaaaatt ggaagtaatt ggcattgccc ttcaatttat tattctgaaa aatcctaaat
     9361 gccgggcata aaaaaacccc cggttccgtc gaagactttc ccgggggatc cccatgaaaa
     9421 cacaaatatt tgtttctttc gaaacagtat tttaattact gcgcaagata agcctttaat
     9481 gctttattgc gtgtgcttga acgtaattta ttgatagcat tgtctttgat ctgacgaacg
     9541 cgttcgcggg taagaccaaa ttcttcgcca atatcttcta atgtcattgc atatggaaca
     9601 ccaataccga agaaaagttt gataacatcg cgttcgcgct ctgttaaagt tgcaagcgtg
     9661 cgctcggttt ctacttttag cgagtggctg taatccagtt tctcgtcggt gcgttctaca
     9721 tcgcggtttt ccaatacatc gagcagtgag ctgttttcac catcatcaaa tggtgcgtca
     9781 acagatacgt ggcgactgga tacgctaagt gtatttttga tttcatccgg tgccaggtcc
     9841 aagatttctg caagttcttc aggtgtaggc tcacgttcga atttttgttc aagctcgctg
     9901 aatgcgcggt tgatcttgct caatgaacca actttgttca atggcaaacg aacaaggcgg
     9961 ctttgttcag ccaatgcctg cataatgctc tggcggatcc accatacggc gtaggagatg
    10021 aatttgaaac ctttcgtttc atcgaatttc tgtgcggctt ttaccagacc aacgttgcct
    10081 tcgttaatca ggtcgttcag cgacaaacca ttgtgctgat actgtttcgc aacagatacc
    10141 acgaaacgga ggttggcttt taccagtttc tcaagcgcaa gttgatcgcc ttgtttgatc
    10201 cgctgcgcaa gagttacttc ctcttcagcg gtaacaaggt caaccttgcc gatttcctgt
    10261 aaatacttct ctaatgactc gctctcgcga ttggtgatgg attttgtaat cttaagctga
    10321 cgcatttctc ttcagacctc ttttgcttta gtgatgaact tgaaccgata aatagaacgg
    10381 aatgctaaag ataaaagttt cccgaattga cggatttgac cgaaccggag ggcaaagata
    10441 ctgcgttttt tacacgaaaa caaggggttt gaggcctttg cttcacctgc aagcacgcct
    10501 ttttggttaa aacttatcaa accgctgtca aaaagcgcgc aattcggttg aaacccttta
    10561 ccggaaaggc ttgaaatatt aaaattatag ttaaagtgtc ctaaaaaatc accactcccc
    10621 aaagggattc gtatttttaa cctacaatcc tatcaaatga aaaaccttca attccaaatt
    10681 ctcatgctcc tgttcgcaac tacagccctc atgcaggcac aacctactat tacttcggca
    10741 acagcaggag gcccgggcga cgagtttacc aataaaatga tcgacgccat actttttgat
    10801 cccggcgcaa gtggtgctga tgttacctgg gatttcagcg gggtaaccct caccggcgaa
    10861 accaatgatt atgctttcat tactgcagcc gctacaggtc agagtgcagc tttccctgat
    10921 gccaacgtag ccgcggacaa tggtatgggc aactatagtt tttataaaat caacgcatcc
    10981 gtttacgaaa atcatggcat ctatacaccg gatgtaacgg tattatattc tgatcctgaa
    11041 gagctgatga cattcccgat aacctataat gctaccaata gcgacgactt ttatagcact
    11101 atcgacgcgg gggttgaaat catccgcagc ggaactgtag aaacagtggc tgatggatat
    11161 ggtaccctga aacttcctga cggcacctac accgatgtga tgcgcgtatt tgtgcatgag
    11221 gtttattccg atgtaaccgt tggagtaccc gtagatattg attatgacat gtatatctgg
    11281 ttttggctga aagaaggtgt ggtaggtcca ttgtttacct attatttttc aagtgtaagc
    11341 accggcggtt ttccaacgat atcgcaagtc gccatgatca atgaaagcaa tagtacagtt
    11401 ggtatcaaag atcaaactgc gcaaaccaca ttaaaggcat tcccaaatcc cgcaaatgat
    11461 atggtgcatg taagcggtgt ttctgcatca gcaaaaaaaa ttatacttac cgatttgttg
    11521 ggtaataatg ttagcgaaat tgaaaatcca accatatcgg aaggaatgat ttcattttct
    11581 agcgcaacaa ttccttccgg aatgtatatt gcacaggttg tagatgaaaa ttcagtgcag
    11641 tcggtaaata ttcaaattct acattgagat aaaggatata ataaaaaagg tgtgttggaa
    11701 atttcaacac accttttttt atttaaaacg atgtatattt ttcaaaaaaa ttcaaccttc
    11761 tgatgctcgc tctaattcgg cgatatcaat ttttcgcata cccatcatcg actgcatggc
    11821 acgcgaggct tttgctttgt cggggtgact taataacatg ccaagttgtt ttggtatcac
    11881 ctgccatgaa acaccatatt tgtctttaag ccatccacaa cgatcttctt tcccaccttc
    11941 agtcagcgca ttccaatagc gatctacctc ggcctgatct tcacaactaa tgaatagcga
    12001 tattgcctca gtgaactgaa aatgtggccc ggcattcaat gcataatacg tttggtcgaa
    12061 taaagtaaat gttgcagaca ttaacttccc gcctaaaccg ggaatcgagt cgctataata
    12121 ttgctttgag acgatcttgg catccggaaa aatactgcaa taaaattcaa cggcttcttc
    12181 agcctggtcg ttgtaggtca aaaaggtggt gatcttttgt gccataatgt gatatttaat
    12241 tttgttgaag gcgaaagtag ccatataact ccggcttctc aaaattcttt catggaatca
    12301 tatttaatct ggaaaaatta acgtttatat cttgcctatg aaaaacctca agttgccaat
    12361 atttgctatt tccattatat cgattcatgc atgtacaaca aagccgaacg tggcaacacc
    12421 ttgcaatgat attttcgaaa caccgaaggt aatacaacat ccaacaaata caccccttga
    12481 aaatttgctt atggatacca tgtcctttgc aaattattgg atcgttgaaa aatacaggtt
    12541 taatgatgaa atatattata gaaagcatcc gcacacgaaa tatattaagt ttaaaagcga
    12601 tatggtagca gaaagtatgt tgactcaatt gttggatacc accaataatc atggctttca
    12661 aataattacc gattatacaa caaccctgta tgattactat ggcgacgacc ttactcctta
    12721 tgcctgtttt ccggtattta ttgtaaatcc aacacaatct acaaaagtgt ttgttgcgaa
    12781 agacagttac gtatttgcta ttcaggaagc aatggatcca cacaatcagc aatggtatcc
    12841 gatagaatgc ctaccatatg atttctgtgg taatggctac tatgggatta aagtgcatcc
    12901 aaaggaattt gtgatgatcg ttgcgccaaa atttcaaggt gacacaaaag gaatgcttcg
    12961 aatcagattg aaaatcggtg aagacatata cgtttcccaa ccgtatgaag gctattttaa
    13021 ttccggacag tttgttccgc cttatttttt gcaaaaacga attgatgacc cggagcgatt
    13081 gaaatatgga attgtgcggg actttatcgg atctgtacca aaggttgtta attgaggtat
    13141 cattccaaaa cactatacat tcgattttga tcttgcagga taaaaaggcc ggtttgcaga
    13201 tagtatcctt tcaaacacca atttcattta agcctggcac cggttggaga tacttttgga
    13261 aaaacatcca atatgtctgc accggaaaat tgtatcgtaa ccgaaaacct gacacatcga
    13321 tttggctcga atgagcttgt tgtacaggat gtaaatctcg tcatcccccg ggcgtcgata
    13381 tacggctttc ttgggcctaa tggcgccggt aaaaccacca cattgcggct catactcgga
    13441 ctgctgcaaa agcaacaagg caagatttat ataaaaggta tgcctttgca taaacacaga
    13501 acgttgttga tgcgtagcat tggaaatctg attgagtcgc cgtctattta tgcacatttg
    13561 tcggcaacgg aaaatgttcg catacagcaa atattgttcg gaactaatag gaagcgtatt
    13621 caacaagtat tgcaattagt cggattggaa aaaacaggtt caaaaccggc cgggaaattt
    13681 tcgctgggta tgaagcaaag actgggtatt gccatcgcct tattgcatag tcccgaaata
    13741 ttaattctcg atgaaccaac aaatggcctc gatccaaatg gcatgattga aatcagggag
    13801 ttgttgttgc gattacataa agaaaatggc atgaccattc tggtgtcaag tcacttgctc
    13861 gccgaaattg aaaaactggc aacgcatgtg ggtgttattc acaatggaat actccgattt
    13921 caaggcagca ctactgaatt ggctgacttg caaagacatc aaaaaggaac cctaatcgca
    13981 acagataata ttagattatc gaagcaaata tttgatgagc agggaatatc ttatcatatt
    14041 gccgaaaatg tttttcacgt gtctgtcgaa aatattcaac gtgttgttga acttaatcgc
    14101 acacttaatg aaagaggtgt aggtgtattt aaaatcgaag cacaaaaaca tgatatagaa
    14161 acaattttta tggaacttat taaatgaaga tgatgatact acaaagcatt aaatcagaat
    14221 ggttaaaaac gaaacacagt attgccacat ggctttgttt aatcggcggt acatttattc
    14281 cacttatttt tctgattggc atgttgaaag atcataggac catcgatatg tatcagggcg
    14341 aactcaatgc atggacctat tacttttttc aggtatggca atatatgcag gtgtttttat
    14401 tgcccatggg gttgatattg gcaacaagtc ttatcactca ggtagaatac agaaataaca
    14461 cctggaaaac ggtgcacacc accccgcagt catatacggc aatttttatg gctaagttta
    14521 tcgtattgtt gggtatgacc ggtctttttt tcttgtattt taatgccacc tggtatcttt
    14581 ctgcagcaat accttcgctg atttttgccg ggcattttcc tcctgttgat tttccttgga
    14641 atcaattttt gacaagtaac ggaaaaatat tgttaacatg tttgcctgta ttggcgcttc
    14701 aatttttatt aagcctccac tttaaaaatt tccttgtgtc catcggcata ggcttattgc
    14761 tggtggttgg ctcattaatt ctgaccggac aatgggaata tgcttatact attccttatt
    14821 catatcccgc attaactatg atgggagatg ctgcgcaatt agaaaaaatt catatctatg
    14881 cctggtgcct gggttatttt atcgtttttt tgaccttcgg atatgtgttg tatgtgacca
    14941 gaaaagccaa agggtaacat aaccgaatat attaccttta agtccggaaa atgaaaaagt
    15001 acgtagtcgt attattgaat cttggatatt ggctggtatt catgctactg atattggtga
    15061 tattcggatt aatatcatcg gtatcaccaa cttccgacga atcagataaa ccggtattgc
    15121 gaaattggct cggttttatg accagcacca cgattttacc cggtgtaatt tgcttttatt
    15181 taaactattt ttttgttttt aacagatatc tccaaaaacg taaaatcccg cagtttttta
    15241 ttgcggtgat agtaacagcg ttaatatgcg gcgtggtggg tgcgttttcg ggcgctgcac
    15301 cttggctcat tggtgatgaa tattatatcg ccaatatgag cataggaaat ttgattgcga
    15361 ttgtcgtttt ctttggattt gtcgccatgc taaacggcgt aataggattg gttatgcggg
    15421 gcttcatctc ctggtatggt gatatccgta tcaaagaatc actcgctgcc aaaaacaccg
    15481 aaatggaatt gtctttggtc aaaagtcaga tcaacccgca tttcctgttt aataccatta
    15541 acaatatcga cgtattgata tcaaaagacc cggaaaaggc ctctgaatat ctcaatcgct
    15601 tatcggatat tatgcgattt atgttatatg agtcaaaacc ggatcgcatc ttattgtcgc
    15661 gcgagatcgc ctatatcaca aaatacatcg atctgcatcg tatacgaagt aataacaccg
    15721 attacgtgca tttcgaagtt atcggcgatc cctctgaata tatggtacca ccaatgttgt
    15781 tcatcccttt tatcgaaaac gcttttaaac atgtgggcga taaaaagaaa acacccgcaa
    15841 ttaatattaa gctgattatt gaaaacaatt ctttgctttt caattgctgt aatcactatt
    15901 ttcctgataa aatacaagat gccgatacat taaatggtgg tttgggcgat aaattaattc
    15961 gtcgccgatt ggaattattg ttcccgaatg cacatacctt gcagttaacc actgacaacg
    16021 aacaatattg cgtaagcctt aaaattatac tttaactatg cgatgtctga tcattgaaga
    16081 tgaaccattg gctcaggagc gaattaaaca atacatcgcc agactgccct ttctggaaat
    16141 ggttggatca tttgacaatg ccatcgacgc cttgctcttt ttgcgcgagc atcatataga
    16201 cttgttgttt cttgatatta atctaggcga aatgagcgga atacaattgc tggaggtgat
    16261 aaagccaaat tgtccggtga tcatcaccac cgcctatcag gaatatgctc taaagggatt
    16321 tgaattgaat gttacggatt acctcatgaa gcccttcacc tttgaacgct tttatcaggc
    16381 tgttgaaaaa gctcgtgcgg ggacacaaat ggttgtgacg caagagaaag cattcatatt
    16441 tgtgaaaaca gaacatcgcc ttgaaaaagt gatgcttgac gatattcagt atatcgaagg
    16501 aatgcgcgat tatcgaaggg tacatctttc ggacaaaagc ataatgacat tgcaaacttt
    16561 tactgaattc gaaaaaatcc ttccacccca aaagatatgt cgcattcata aatcgtatat
    16621 ggtttccatc tccaaaatca cttcaattga gaaagatcgg gttttgattg ccggaaagcg
    16681 actccctata tctgagacct acaagaaatc ttttttggat atcattggcg caaaataaaa
    16741 aatgaccgaa ggatcacgaa tgattcccgc ggtcatttga tgttttggta attgtgtctt
    16801 tattgttccg acttgcaaca aggatgcaag ttgtcatacg ctaccttatt tgcaggcacc
    16861 tcatcagctg tataaccggc ttctgtaatg gcagttttta atgcgtcggc atttgtctta
    16921 tcgccatcga atttaatgct tactactttt gtttccagat ccaggtcggc tgattttacg
    16981 ccatcaactt tttccagagc agtttcaata gcagccttac acgtgccgca cactgccgaa
    17041 gttttaattt cgatatattg ttttccgcta ttttgtgcaa atgacgtttg catgcctata
    17101 gccatgaata atccgataat tgcaagaatc tttttcataa tattcaaagt taattttttt
    17161 atcaattagt taaaaatata tctcaacccg ccgtaaaaca tacgcggcat cagcggggca
    17221 taaatgacgc cggcatcaaa gttttcacca aacggatctg tagcatcaat tatgggattg
    17281 tgctgggtat acccggtaat attctccgat ccgacataaa catccaggtc gccgaaagat
    17341 tttgtcacct gggccaacag ttgaatataa ggctcagaat tcgtgccaaa gtcatgtgct
    17401 gcgtggtttt ctgacaggtc gggtaggcgc gttttgccgt agaattgagc cgtggcgtct
    17461 gctgaaagtc ccgccttttt ccagttatag gcgacattga ataagccgcg atctttaaag
    17521 gtcaaaggca cttctaaaag ctttccgtca taagtcgctt ttgaatctac atacttatat
    17581 gcagcgcgca actcaagacg ttttattggc tcataattta tttcagcctg aagcacattt
    17641 gtatatgctt cgcccttaag gttgtagaca ttgatctcat ttacattagc atccatatct
    17701 atcaccacct ggttgatgaa atcagtgcga taaacatcca gggtgaatgt tccctctctg
    17761 tgattaagct taaactgctg gatcaaagaa ataccataat tccacccctc ttcaggctct
    17821 aatggttcct gaatgatgag ggtgcgattg ctggtaagga tactgctgtt ttctatcagc
    17881 acattggcta tatgataacc ctttccggca gaagcgcgca aggtggtgtt ttccttcggc
    17941 gtaaatttca catgcatcct gggcgttaca aaagtgccgt agagattgtg ttgatcgaca
    18001 cgcaatcccg ccaatacact ccattttttt tcgaactgat aatggtattc tgcaaaaatt
    18061 ccgggcaccg attcgttgcg cagataggtt atagaatcaa atgattcatc gaaattatca
    18121 tacagataat tcaatcccat ttttatagtg ctgttggtat tgccgatata ggtaacaaaa
    18181 attgcttccg ctttaataaa ttgttccgca ccggcataat tattcaatcc ataaaaaccg
    18241 gtttgattat gatcggcaaa ttgcaatata gtgcctatag atgtagaagg acgtttaaac
    18301 acaaatccat ttttcatgaa taattcccat cgcgaaatat cagcaccaac gccatatccg
    18361 ttttctgtag tccgcggact gttaacatca aatgaggttt gtccgccggt aacgttgctg
    18421 ttgatatatt tcacgccaaa ttgcgcctcg tgcatgccgc ccgaaaaata attccactta
    18481 ttcatgaaag tgtaagtact gttcatcggt acatctaaaa atccatcatc attatgatct
    18541 actttttgtt gcatctgcga aacattacca tataatcctg tactccattt aagattaaaa
    18601 ggatgcttat agacagcgtt tgcttcccat ctgccaaatt gattgccgta tacatccaga
    18661 cgtgctgttt cttcatcggc atcaaacggc tttttgtatt cagtgttgat ggagcctgtg
    18721 atgccctcgt agccatttac tacagagccc gggcctttag tgatttgtat gctttctatc
    18781 catggaccgg gtataaatgt cagaccgtac gcggaagaca atccccgcac attatccatg
    18841 ttttctgtca ttatttgtgc atacacgcca tcgagcccca gcaatttaat tgttttcgct
    18901 cccgtaacgg cgtcgctgaa ttcggcatca acggtattat tggtttcgaa gctttcactc
    18961 agattacaac atgctgcttt tagcaattct tttttattga gttgttctgt gccgatagga
    19021 tcgaccgtgt taattgtttt tgacgattgt gcgccttcaa caacaacagt ttttaattct
    19081 gtatcaggcg ataacatcac atgtatatgt ttagttgttt gcacatcaaa ggtatcggct
    19141 gaaaatccaa cataactcac aaccagatag cgttgtgatg ttgttttttg ttcaattgaa
    19201 aaatggcctt catcatcgct gataacgccg gaatattcac cctcccaata cacatttgca
    19261 cctggaaggg gaatcatttt tcctttgtcg tcgttcgtca tcacctcgcc ttcaaccatt
    19321 tgttgggcaa aaaccggggc agccataaat gctgaacaga taaaaagcag tgatgaaaga
    19381 atgtaggtcc taaaattttt atttattgat atcataagta attgattgaa atgaaaaatt
    19441 tcccgcccct ttggcaggtt caccattttt cattgcacat caaatacgat agatacactg
    19501 catcaccctg atatcgccgg gaaacggagg tggcaggtta aaggagttga ggttgactac
    19561 atcagcctga atttcatcct gtatttgaat tacaggatag tccaaatctg catagtcggt
    19621 atagccaacc tgattttgaa ctgagcccga gtattcaaac ttgataaact gcgtcttttc
    19681 aatacagcag ttcatagaaa gggtcaccgg agcatccggg agctgattgc cacagcaatc
    19741 catagacata gaaacaggcg caacacccga ctccccggtc atcagacaga ggtgggtgaa
    19801 caggttgaaa ccacttgtta tcccaaaaag gagcacaagc atcatcatcc caaatatggc
    19861 gcgcagacgc ttcattgttg caaaattacg tgttttgaca gcattttgac catttccgac
    19921 caaacgaact gacaaaagtc agcgtttcat tagtaataac ggctaacttt gaacattagt
    19981 ttacaaaaat tatacccgaa acgtcaacta tgaaaagaaa ttttggatct gccctcctcg
    20041 cgatctgcac actgttaaca gtttcctctt gcagcaaaaa agaaggttgt actgacccga
    20101 ctgctaccaa ctacgatccc gatgccgaaa agaatgttgg ctgtctgtat gaagaaacct
    20161 atgccgtaga aatgcatgtg caccagtttc tcaatgatat tgaactggag gaagaaatgc
    20221 cttatgtcat taacggcgta aacaccaacc tcaacctggc gcaattttat gtaagcggaa
    20281 ttaccctggt ggatgctaat ggcaatgaat ttaaacaaga cggattatac ctccttgttc
    20341 aaccggagac agaagagtat gccattggag attttcccgc aggcgattat acttctgtaa
    20401 aatttgatat tggtatagac tctgcaacca accatggcga cccatcatta tatccaatcg
    20461 gcgaccctct gggggcacaa acaccaagca tgcattgggg atggagcttt ggatacattt
    20521 ttatgcgtat tgatggtgaa gtggattctg atggcgatgg cactcccgat cctgcaggtg
    20581 cttttgagat gcatttggga acagatcact acctcgcgtc tatcgaagta gaaatgccga
    20641 tcagtattgg acagggactc gaaaatattg ttcacctgca agcagattac accagctttt
    20701 tcaccggtgt tgatatggta aacgataata cgactcacac tacagataat gtaacattgg
    20761 cgaatttgtt attagataat atatatacca tgttctcgcc cggagaagaa taatggtatg
    20821 taataccatg cgtaaattaa tttctatatt atttctcagt gcaatattgg tatcctgttc
    20881 tgaagtgata cctgaagaaa aggcatatga gttgcaaatt cctccgggat ttgatgtgcc
    20941 gcaaattcct gaagataata tcctgacaga tgcaaaaatt gcactcggaa aaaaattatt
    21001 ttacagcacc gcattaagtg tcgattcaac aatctcatgt gcatcatgtc atcgtcctga
    21061 aaatgctttt gccgatcatc ttgctatcag tcacggtgta atgggaagaa caggtttccg
    21121 caatgcacct tctttacaaa atgtcgcatg gtatacggtt atcacaatgg atggcggaaa
    21181 tccttcactc gaaacacaac cttatgttcc cattgaaacg catgaagaaa tgggatttaa
    21241 tatggtattg ctttgcgagc gattaaatac cgatgcggaa atggtgacgt cattcaatga
    21301 tgtgtttggt tccaatcccg atccatatgg cgtaacacgc gctttggcag cttttgagcg
    21361 caccatgatc agcggaaatt cgcgctacga tcaatatgcc tatcaaggaa acattcatgc
    21421 actcactgca gaggaacttc aaggaaaaga tttgtttttt agcgatgcat taggatgcgc
    21481 aaattgccac agcggatttt tatttacgaa taatactttc gaaaataatg gtttcttttc
    21541 cgactatagt gcagattccg gtcgcgcacg tattacatgg ttgcacgaag atgttggtaa
    21601 attcaaagtg ccatcgttgc gcaatgtggg tgtaacagcg ccgtatatgc ataatggtca
    21661 ggtagaaacg ttagatgaaa ttattgacat gtatgcggca ggtggatcag gacatgaaaa
    21721 tcaaagtgta tttattacag ggttcagcat cacacctgca gaaaaggaag ctttaaaagc
    21781 atttttatta tcgttaacgg atatatcctt catgcaaaac ccggatttgc tggaataaaa
    21841 tttaattaac aatattacct ttaccataat gtttcgcaag catagaattt ggatattgcc
    21901 aattgcattt tttgcaacta tcatgttgtt cactaaatgc gatccggatg atcctattac
    21961 accggatgat gaatgggttt atgatgatac gccttacact gctgatcacg taaacggtat
    22021 tccacccatg cctgtgttta caacaaaccc gcttacggtg aaaggtgttg aactgggaag
    22081 aatgttgttt tatgatccga tattgtcggc cgacagcacg ctatcctgct ccggatgtca
    22141 tagcgtggcg catgcattta gcgacactac cgcattcagc atcggtatcg atggacttcc
    22201 cggaaaacgc aatgcgatgc ccatttttaa tttgggatat gccgttgcca taaatgcaac
    22261 atttttctgg gatggcagag cagcaacaat tgaagatcaa accttaatgc ctattcagga
    22321 tccgctggag atgcatgaaa tattgccaaa cgtaattaca aaaattacca gaagtgaatt
    22381 ttatccaaaa gcattttatg aagcatttgg tgaagaagta atcactaccg aattagttgc
    22441 acgcgcatta ggtcaattcc ttaaatctgt caccagtgga aattcacggt acgatcaggc
    22501 aatggcagga gaaatatttc ttactgatga agaaacactt gggtttacat tatttaatgc
    22561 attggatggt gccgactgtt ttcattgtca tggtgtaaat ggcggattgt ttacggatta
    22621 tgtatatcgc aacaacgggc ttgatgatgc aacacattac accgattttg cggatgcggg
    22681 acttggtgct atcagtggcg atacggctga ctatggaaaa ttcaaaacac cttcactgcg
    22741 aaatattgaa ctcaccgcac cttatatgca tgatggaaga tttgccaccc tggaagaggt
    22801 gctcgatttt tatagtgaag gcgtgcatga tacacctttt acagataatc tcattcaatt
    22861 tgcatatcaa ggtggtgttc aattgacggc agaagaaaaa gcggctatca tcgcattttt
    22921 gaaaacactt acggatacca atttcgaaac gaacgaagca tatcaaaatc cattctagac
    22981 tttaataggt ggtcagcatt caattttatc gttagagcgg tttgcttccg ataaccccca
    23041 acatattgtc atctcccgaa acccttatgc agcaaggatt ttgaagttta tgtatactta
    23101 aatcaatcta tatagcgttt aatatcgtat acaagtaacg atacgccgtt gattttgtct
    23161 ttggtatagg atctagtgta ttgcattttt gtatagcaag ttaaacgtat gttcaccgac
    23221 agaaatatag atatcttcct cgcaggcctt gttacccgcg tggcgttgtc gtttccgatc
    23281 gcgtatatac gttctattgc acttgtcagc aataacaaaa agattgccga ttgcaactgg
    23341 gagtcagatc ctcgcggaac cgtaacgcgc tacgatgatc tgcatgtacc ggttgctgca
    23401 gaacaggtag tggtagaagg cctcgacacc aatggtcagc cgctcatgat taccgctgca
    23461 atagcaagcc ttcattaatc atactgtcct tcacaggaaa tcaaagagca cgaggagttg
    23521 tgtgttcatg tgttcgtgtt cgggttgatt tgtggaatgt gcttagccgg caatccgtgc
    23581 aatcccttaa tcccaataat ccgtgattca gacaatgatt gtgtgcgggc ataccagact
    23641 tcgcgccttg gcctctttgc tgtcttttcg cgaacccgcc tcgctactgg cctttccatt
    23701 aaacatagaa agggtgcaaa tatcactgcg gaataaaatt tttcaaaaga atataattgc
    23761 attaaccatt aacgcgtcag cgttaaaaac tcccgcacct gctaacttgc taaccttcaa
    23821 acttgcagat tgggaggcag ctatcacccc gatttagatt ctcacctttt tcacgcagag
    23881 ccgcagagtc gcagagataa acatgcaagc attcaccccc atttgctaat ttgctaactc
    23941 tcacacttgc taattaccct cacttgctca cttgctcact tgctaactct caaacttgct
    24001 aactaagacg agcgtcttca tgcgagcaca ccccccatta gctaatttgc taattccact
    24061 ccctctttcg tcgtaacttt gccccatgga ttccgcccag tctaatttcc aacgcgtttt
    24121 acagtttgat cgccgccatt acagcaacga tcagctgatt cagatatata agcgtttgct
    24181 caaaccccgc atgatagagg agaaaatgct caatctcctg cgtcagggac aagtaagtaa
    24241 gtggttcagc ggaattggcc aggaggctgt tgccgttgcc gtgccttatg tgctggaata
    24301 cgacgaatat atcctcccca tgcacagaaa tcttggggtg tttaccggtc gcgacatccc
    24361 ttttgccaaa ttattcagtc aatggcaagg aaagaaaaac ggattttcta aaggtcgcga
    24421 acgaagcttt cactttggca caaatgagta tcatatcgtt ggtatgatat cgcatctggg
    24481 acctcagttg gcagtagccg atggcatagc acttgccgca aaattgaaaa acgagaaaaa
    24541 agtaacagca gtattcaccg gtgaaggtgc tactagcgaa ggcgattttc atgaatcatt
    24601 aaatgttgcc gccgtttggg atctgcctgt aatctttatt gttgaaaata atggttacgg
    24661 attgtcaacg cctaccaacg aacaatttcg ctgcgcacaa attgcggata aggcgattgg
    24721 ttatggtatg cgctcaatga ttgttgatgg caataatatt ttggatatgg ttgccgcact
    24781 cgaagaatgt cgcgcatatt gtatcaatga acaaaaaccg gtgctgttag aatgtatgac
    24841 attccgtatg cgcggacatg aagaagcaag tggtataaaa tatgtgccga aagaattaat
    24901 agaaatgtgg ggtaaaaaag atcccgttca gaattacgaa caatatttac tggagtcggc
    24961 cattattaat gaggagatga ttaaaacctt ccgcgatgaa attaaatcag aaatagaagc
    25021 gggagtgcaa acggtattta atgaaccgga agtaattccc gataccgaag aggaagaacg
    25081 cgatatttat gcaccgttta cgcaagaagt tatagcgccg aaatcaaata ttgcttcaca
    25141 aaagaaattt atcaatgcca tttccgatgc gttaaaattg gcaatggata aatatcctga
    25201 actcattatt atgggtcagg atattgcaga atatggcggt gcatttaaag taaccgaagg
    25261 ctttgttcag cagtatggaa aatcacgcgt ccgcaataca ccaatttgcg aaagtgcaat
    25321 tcttggcgcc ggttgcggac tgtcggtgaa gaaaatgaaa agtgttatcg aaatgcagtt
    25381 cgccgatttt gtaacgagtg gattcaatcc cattatcaat aacatggcaa agatgtacta
    25441 ccgctgggga caaaatgtcg atagcgtagt gcgtatgccc acgggtgcag gtgttggcgc
    25501 aggacctttc catagtcaaa gcaatgaagc ctggtttttt catacgcccg gattgaaagt
    25561 ggtatatcct tcaagcgttt atgatgcgaa aggattgttg ctcgcggcta ttgaagaccc
    25621 gaatcccgta atgtttttcg agcacaaagc attgtatcgc gccatcagcg ataatattcc
    25681 tgatgattat tatacggttg aaatcggtaa agcagcattg gcacaaagcg gaaatgaact
    25741 aagtatcatt acttacggta tgggtgtgca ttgggcgaaa aaagcttgtg ctcaattgca
    25801 aattgatgcc gatatacttg atttgcgcac actcttgcca ctcgattatg atgccattgc
    25861 aacaaccgta agaaaaaccg gaaaagtttt agtgttgcat gaggatacct tgattggtgg
    25921 tataggcgga gaaattgcag cctatatcag cgaacaatta tttaatgtac tggatgcacc
    25981 ggtaatgcgt tgcgccagcc tcgatacgcc ggtgccattt agtctcgaac tggaaaagaa
    26041 tttcttgcca gaatcgcgtc tcattgaaaa attaaaagcc ttggcgtctt attaatttga
    26101 aaggattcca tatcgacctg aaattaaaat agtacatgtc cattcacctg caacatctcg
    26161 aatcagaact ggaggaaaac acccgccggc gtggatacga tttttattgc gaagggagag
    26221 tgcgttctgt atttaatatc aatggcatag agtggatggc agtagtgcag gggtacgacg
    26281 atcatgttgt ggaactgcgc atcaccaatg atacggtgat gcattatgcc tgcaattgta
    26341 atgatgaaga ggatccatgt aaacatgttg ctgcggtgtt atattatatt cgaaaagaat
    26401 cgcacaaaca aaaacgcaac gatccttcgg gctatgatga aatgaaggag gaaataagtt
    26461 atctcccacc acatgtgttg cgttatctca ttttaaaata cgcagcccaa agcaaagatt
    26521 tcaggacctt tttgggcgaa tactttaacg gcgataccag aaaataagtc gcacctgatc
    26581 attattattt gttcatgctt tttgaataat gcgtgaggtg atataagtcg ccattcttag
    26641 tgacaatatc catactccgc acgataattt gcgtcgatat ttgacgtata aaatctaaaa
    26701 gccatgaaca atgaacaagc aatcggagca tcacgcaaac atattgctga gctgcatccg
    26761 gaagtaatag attggattaa ctattccctt tttgtaaaag acgaagtaaa atcactcaac
    26821 catcgattga tggaaatagc gcatcgttat accgatcatg atgtgatggc acaggtagaa
    26881 cactttcaaa accagtttat tcgtcaggat gaggccagcg
//
LOCUS       DAOQHY010000019        26815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_790_length_26815_cov_5.442451, whole genome shotgun sequence.
ACCESSION   DAOQHY010000019 DAOQHY010000000
VERSION     DAOQHY010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 26815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26815
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_790_length_26815_cov_5.442451"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1381
                     /locus_tag="PLL28_02905"
     CDS             <1..1381
                     /locus_tag="PLL28_02905"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNF68293.1"
                     /translation="GLGGKKVRDIWTQLGIETVGELLYACYENRLSKLKGFGEKTQAA
                     VIQSIEYYQNNLGKFHYAAVAEYCDNILRQIHDLISDKRIMFVGDLRRKCNIINAMEI
                     IAPASADISSHISNINEIVVEESSQSLIKGKTAQDMQFSILIVSDEDYVMQLFRLTGC
                     EEHVNMILSLAGQGPFASEADIYSAAGLPFVFPEMREQFAEVDLMQFLHKATSPKHQL
                     IELSDIKGVVHNHSTWSDGKNTLEEMANACVEAGYDYLVISDHSKSAFYAGGLTEAQI
                     VQQHSEIDKLNKQFTDFHIFKSIECDILNDGGMDYDNDILATFDLIIASVHQNLKMDI
                     EKATTRLLRAIENPYTRILGHMTGRLLLSRPGYPVHHKLIIDACAANGVAIEINANPY
                     RLDIDYSWIPYAADKGVLISINPDAHSVEGIWDIRWGVQSARKGGLAGNQTLNTKTLQ
                     EFRNWLERK"
     gene            1447..2295
                     /locus_tag="PLL28_02910"
     CDS             1447..2295
                     /locus_tag="PLL28_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HNF68294.1"
                     /translation="MKKYFSLITFSHTVFALPFALIGFTLGITQPGVQFEWLDLVFVL
                     LCMVFARSAAMAFNRYIDADIDKDNIRTASREIPSGKISKSKALQFTIINCIGFVISS
                     GMLNVLCLYLSPIALLVILGYSLTKRFTWLCHFVLGIGLSLAPIGAYIAVTEHFQTLP
                     IIFSLAVFTWVSGFDIIYALQDEEFDKAHKLYSLPAKLGKFNSIIVSIIVHLNTVLLL
                     WYAGWYIEFGTIYWIGMGLFTTALVYQHLIVKPDDLSRVNRAFFSANGIASVVFGIFV
                     ITDLLV"
     gene            2298..2708
                     /gene="ruvX"
                     /locus_tag="PLL28_02915"
     CDS             2298..2708
                     /gene="ruvX"
                     /locus_tag="PLL28_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNF68295.1"
                     /translation="MARILGIDYGTKRVGLAVTDPMQIIASPLTTVDTPQALDYIAKY
                     IAENEVERILIGYPTDLQGRPTDATFHVEGFIKAIQKRLPEMPLQKWDESYTSRMAMQ
                     SLVASGIKKKQRRDKKLLDQVSAAIILQEYLQNL"
     gene            2710..3285
                     /gene="def"
                     /locus_tag="PLL28_02920"
     CDS             2710..3285
                     /gene="def"
                     /locus_tag="PLL28_02920"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNF68296.1"
                     /translation="MILPIVAYGHPVLKQVAQPITADYPDLAKLIANMWETMYNGRGV
                     GLAAPQIGLDIRLFVVDSEQLKDDDDEEDDDVRLTKGIKKVFINAQIIEETGEPWSYE
                     EGCLSIPDIRVKIMRNETLRIKYVDEHFVEHIETYDEMNARIIQHEYDHIQGVLLTDH
                     MTPLKRNLIKKKLDNIATGKISTKYKMKFAK"
     gene            complement(3309..3935)
                     /locus_tag="PLL28_02925"
     CDS             complement(3309..3935)
                     /locus_tag="PLL28_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNF68297.1"
                     /translation="MRKWISILSLFTCISLRAQVATTDSLIQFSGLVMTSDSLMAVPY
                     AGIWSLTRNNGTTCDYNGFFSFVAAKGDSIRISALGYKDQVFTIPGDLTDTRYSVIQL
                     LTRDTIFVAETIIYPWPSKEEFRDAFLALDIPDDDYEVARKNLDREKLKDLGEAMAMD
                     ADMNADYQTKAIQQKIYYAGQYPPMRIFDVFAWKEFFEAWKRGDFKKE"
     gene            complement(4017..4241)
                     /locus_tag="PLL28_02930"
     CDS             complement(4017..4241)
                     /locus_tag="PLL28_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68298.1"
                     /translation="MKKLSGPFVLAAILAAHIGFVVATEESYTFRDDDAPSIKTEAPA
                     VDVSFSTLKGGSTANPNAMVSLTDNNTRIN"
     gene            complement(4812..5315)
                     /locus_tag="PLL28_02935"
     CDS             complement(4812..5315)
                     /locus_tag="PLL28_02935"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68299.1"
                     /translation="MNRILITLAFLLTSAVMFAQTNARERSEDTNLPEIDNLSGLQPD
                     INDLNPSVATEVSTGSLENGNTEVGDEGASHTGSYTGDNIKLEGTNSLEEAQVYPNPA
                     TDYIYVNSGLSDGTIHVLNLLGQDMMQVAITSPVMSLNISELKEGIYFVSIENGANKV
                     VKKIKVL"
     gene            5584..7038
                     /locus_tag="PLL28_02940"
     CDS             5584..7038
                     /locus_tag="PLL28_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68300.1"
                     /translation="MFQNKSIVILRTFTNQEVRQFDEYLRSPFFNKNQTIVQAFDIIK
                     SWHPLYDHPNLERTQLFKLIFPNDTFEEQKLRYLMTDLTKHLEEYLCYKAISDENIFK
                     YHLLLYTYRIRRLDKAFLNTLKLAEKAIKDQPRRDVSHAFYEYLIEEDRYRFMSDRRE
                     HLHETNLQQVVDNLDKYYILNKMRYSAEIINNKNVVAINYRLFLYDEIMNHIRHNPLD
                     YVPAAKIYYNIILTLTEPENKIHYTNLLDLLKEHHQLFSKEEIFEMYVYAKNYCIRKI
                     NHGDIEYIRELFDLYKVMLKDRIIFKDNYLSQFDYKNIVYLGLRISEFDWVQQFIVKY
                     NEHLDPAIRANAYTYNMAYFHFFQNNYDEVLTLLRSVEFTDFYYHLDSKSLLLKTYFE
                     LEESEAFFSLIEAFKVYIKRNNLIPAYQKSGYTNMIKLTSKMFKWKLNPRNDLQDLVD
                     EFNNTKPVVDVLWIKKKLEDLQNRSEKLSGSWRK"
     gene            complement(7087..7692)
                     /locus_tag="PLL28_02945"
     CDS             complement(7087..7692)
                     /locus_tag="PLL28_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68301.1"
                     /translation="MKVKQMLKKNNINRYLLKNPVLLNGVFLFTILSWQACNNSADKK
                     SAEIYKQYPVYKTSDLDPEELSPLQFLQALSDEKSNILYVGLYPNGVTDNNIDFTAIK
                     KVPKNWIKPEHIDSLIIKFNDNTLTIPVFSVYADFAPKPGMHTTIGIEAMRMVEGYRN
                     NMYPSLWPEYYAEDINSIALQDKKQELINWWRQNSNQIVEH"
     gene            complement(7661..9586)
                     /locus_tag="PLL28_02950"
     CDS             complement(7661..9586)
                     /locus_tag="PLL28_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNF68302.1"
                     /translation="MSPETLKLTMIIVGIILALVFYKFILRVFFGMVIVPEDRIGLVT
                     KKFVLFGANKELPDGHIIATKGEAGFQAHSLAPGLYFWKWIWQYEITMQPFIIIPTGQ
                     IGLVMAKDGVELETGRVLARKVECDSFQDAIAFMDGGGRKGRQTAIIAPGSYRINTFL
                     FDVEIVSMTNIPDNCVGIVTTLEGQPLDDGQIAGKIVEGHNRFQDADGFLINAGLKGL
                     QEQVILAGSYFLNPWFVKMEIVKMTEIPIGYVGVVISYVGPEGVDLSGTEFKHGNIVS
                     KGQKGVWADALGPGKYPINPYIMKVELVPTTNLVLNWASARTESHQLDKNLSTITVRS
                     KDGFTFNLDVSQIIHIPTYEAPKVIARFGNMANLVTQVLEPTIGNYFRNSAQDADVID
                     FLKMRKERQESAKQHIGKVLEQYNVNGVDTLIGDIIPPESLMKTLTDRKLAEEQKVTY
                     DTQMKAQETRRTLEKETAVADMQKELVKADQGVVIAEKIADASVKKATGDANGIRIQS
                     TAESDRLKLLANGEAEKIRVLANAEGEKVKLLADAEAERISKTGNAEAEKILAIGKSN
                     AESYKLSVEAMGGDNFTQLKITENIGVNKIKVIPDVLITGSGDSANGPISGLLGIELM
                     KNLAKRVNEGETDAKKE"
     gene            complement(9710..10435)
                     /gene="bla"
                     /locus_tag="PLL28_02955"
     CDS             complement(9710..10435)
                     /gene="bla"
                     /locus_tag="PLL28_02955"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_132050774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B1 metallo-beta-lactamase"
                     /protein_id="HNF68303.1"
                     /translation="MCLLAGFQCSTTGKVAFKPVEVYKTDALRITQISKHAYIHTSYK
                     QTDDFGLVPCNGVIITDKSECIILDTPVNDSCATLLINWVKQNLDVTINAIIPTHFHD
                     DCIGGLNAFHEQHIPTYAYTRTIEFAKQNNITIPEHSFSDSLELPIGDKKIIVKFFGE
                     GHTRDNVVAYFPAENILFGGCLIKSIDAGKGYLADANTAAWPETVHHVKTAFPEVQIV
                     VPGHGDYGDQRLLDYTIKLFSTK"
     gene            10621..11802
                     /locus_tag="PLL28_02960"
     CDS             10621..11802
                     /locus_tag="PLL28_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68304.1"
                     /translation="MKMLLCSLLLGISINLPAQDASILQEKWSYHLINQNFSRPTCAV
                     EFPNGRIMVGIYHSMMNNYNEFSTILVLDSAGNKIRSVVLSDTMNNYVYDMSLASDTT
                     AIIVGKAGPLGQNGTIPGDIYIAKLNVSGDIIWSRKYGSSDADEAKAVIPDGQGGAYV
                     CGRVGFGDGDVSTYYGNRNSADIWTLHIDASGNILWQQTMGGSSYDRPYAMLKLADGG
                     LLILGESASYDGNIESCEGGFDALMFKLDTIGNLVWKYSRGTKYNDTFIAAMVTDSLI
                     TAQLVQSEYVVRTGKTKREEFTITMDQHGEVLSQRKSDPDMIFENGEAYYYFKKDPDD
                     ENSTRCLTVADAEPRRTYYFPWEAQTQLEEVLQLHNGDLLLLVGVYVRTLNAFIVRIE
                     N"
     gene            complement(12011..15466)
                     /locus_tag="PLL28_02965"
     CDS             complement(12011..15466)
                     /locus_tag="PLL28_02965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HNF68305.1"
                     /translation="MKTLYLKSLFLLALVTSGSLYGQSLAMDWQKTIGGTDYETADIV
                     LMTPDHGFIFGSSSLSGISAYKSEPSYGGADYWIIKTDSAANVEWETTIGGSGDDYLN
                     YLSLTDDGGYILGGTSNSEISGVKTSNTIGPYGNPDAWVVKLNSTGHIEWQKVFGGGY
                     DDFVGSINQTPDGGYILGANKEQAGSFIYNYNVYKLNNIGTIEWQREYYAFWDDQLVR
                     AIQSDDGGYLLCGYSNSDITGDKTEPMIDVTDFWIIKINSIGDIEWQNVIGGTHFDNC
                     VDAIKTADGGYLLAAESQSPAGYDKSENSIYYYDYWVVKINATGEVVWENTIQGNNLD
                     YLRCIIQAQDGGYMLGGKSASSIGYDKNSNTYGDYDYWIVKTDESGNILWQKVFGGTN
                     DDDLTSMTITNDGGVLLSGSSRSIVTGNKTAPNLGNYDLWLIKLTPDFSTISGTLYAD
                     LNANTLHDAGEPPLTYKPVYISPEGLTEFSDSSGYYAFQVTSLDTFSYSSDTLPYYTS
                     VPANYTTVIDSFYTVDSLRNFGFQPDSVMNALVIDITPIYLFRPGATVKYTLSYQNIG
                     TTSINGQINLIYDPLLSFMSADLAGYTLYDDSITWSLGTIAPFESGLITVTFIVSETA
                     DAGDMVTSSALIYSPDVYSIVDNSISNSVNEIIASYDPNNMIVNLVELTPEELAVTDH
                     LNYTINFQNIGTDTAFNIIVNNILPVGTDISTFQLITTSHFLSDLEYYADSRLLKFTF
                     NSILLPDSTTNEPGSHGYIQYSLSPDHTLTIGDSISNYAGIVFDFNDPVYTNFATTNI
                     VCPKFHFYADTDGDGYGDDALDTLACDAPEGFVENNLDCNDGNLLIYPLSSEICNALD
                     DDCDGIADEGLVTYFLYADMDDDGYGDADLDTLTCSDIIGFVYNNLDCDDLNTEINPA
                     QSETCNGIDDNCNTLVDDDLTVYTLFVDNDGDGYGDPAFVIDTCVMTVFGFVSNALDC
                     NDTNMSIFSGAEDICNGLDDDCDGFVDENLTFLNQFQDLDGDLYGNWSIDTLACMDIP
                     GYVYDSTDCNDIDPNIYPGATELLNGLDDDCDGFSDEDLGMNSNNSEQLIIYPIPASD
                     IIFVDVASNEVSTLNIVNLRGEIVYQTSTWTGEVISISQLPPAMYMLQITTTNNHFAG
                     YFLKK"
     assembly_gap    15560..15659
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15906..16508)
                     /locus_tag="PLL28_02970"
     CDS             complement(15906..16508)
                     /locus_tag="PLL28_02970"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNF68306.1"
                     /translation="MIILIIALGIIMLPVVYNVFFPLKKPDLSNYFKPGQTYSSKGEG
                     ITQTIIRQEDNKVYCELRFAPFAPGPPEHMHEQMPETIQVVQGTLSAKINGEVVQIHE
                     GERVNLPAGVFHKIFNQTDKEVVLRPQLPEDHIPVEFAYSLDQLYPLMTSKNLTLKMF
                     AKICVLDELFDSIPVGPPPAVFRFIKKAVKPYARLFGVKP"
     gene            complement(16746..16949)
                     /locus_tag="PLL28_02975"
     CDS             complement(16746..16949)
                     /locus_tag="PLL28_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68307.1"
                     /translation="MVSHDHDGDWQFMCGIQAHTMANAKIITYFEALELDPTLLELEI
                     LPVGHHALRSSKDDAWQIIPIKS"
     gene            complement(17318..17833)
                     /locus_tag="PLL28_02980"
     CDS             complement(17318..17833)
                     /locus_tag="PLL28_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68308.1"
                     /translation="MKNALLLLFIGCLLSSCSGFSNASKHIENTEPEYVIQDTFLITG
                     FYRISNNNEGLKRTQLYDTATYYVIPTPVISVKHFKSIEMAKGYYGHKVLSLQFDKMG
                     TDLWFAGTKEAQGKKIAVIVNDTLISAPRVNEPIPVGRASLDLGPENEIKLQQFKSDI
                     EKEMEKYASRK"
     gene            18649..>21470
                     /gene="gcvP"
                     /locus_tag="PLL28_02985"
     CDS             18649..>21470
                     /gene="gcvP"
                     /locus_tag="PLL28_02985"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HNF68309.1"
                     /translation="MKLRINTSETFAQRHIGPNSNDVQAMLQAIGADSIDSLVNKTVP
                     ENIRLKRPLNISAPLSEYNYLKHLRNLASKNKVFRTYIGMGYYNTITPSVIRRNIFEN
                     PGWYTQYTPYQAEIAQGRLEALLNFQTMVSDLTGFEIANASLLDEGTAAAEAMIMFFH
                     HKNKKDVTSPKFFVSELCFPQTIEIIRAKAEPLGIDVVVGDHRNAQIDDTYFAALMQY
                     PAGNGMVFDYTSFIAHLHTHNVSSIVAADILSLAVLQTPAAMGADCAVGNTQRFGVPM
                     GFGGPHAAYFATKDAFKRDIPGRIIGVSVDRDGRPALRMALQTREQHIRREKATSNIC
                     TAQALLAIMAGMYAVYHGADGIRQIAEKVHGLTTVLDNGLKQLGFTQSNAQYFDTVHV
                     ACGSLSGLVHQHAIAKHMNFYYHQNGDVQITLDETTELSDVQDILSVFAEAKGGATVA
                     LPSIDSLQSSLPASSLREDAYLQHPIFNTFHSESEMMRYIKRLENKDLSLTTSMIPLG
                     SCTMKLNAASELLPITWPEFSAIHPFVPAEQVEGYLHIIKELEQDLCEITRFDGCSLQ
                     PNSGAQGEYAGLMVIRAYHIHHNQSHRNKVLIPASAHGTNPASAVMAGMDVIVVKSDA
                     EGHIDMDDLNAQAELHKDDLACLMVTYPSTHGVFEETIMQICNIVHQHGGLVYMDGAN
                     MNAQVGLTSPAKIGADVCHLNLHKTFAIPHGGGGPGMGPICVNEKLVPFLPGHPLFKT
                     GGINAIKAINAAPFGSASILLISYAYIKMLGGEGCTNATKYAILNANYLKSKLDKAYD
                     VLYLGKSGFVAHEFIIDFRKWKHTIGLEVEDVAKRLMDYGFHAPTVSFPVAGTLMIEP
                     TESESKEELDRFVEAMLGIRSEIAALENGTADKLDNVLKNAPHTIREVLSEHWTHGYS
                     RDAAAFPASYIRERKFWPSVARINNT"
     assembly_gap    21471..21570
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            21711..22685
                     /locus_tag="PLL28_02990"
     CDS             21711..22685
                     /locus_tag="PLL28_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HNF68310.1"
                     /translation="MNKGPISQFITHHYRHFNAAALVDAAKGYETHLLEGGKMMVTLA
                     GAMSTAELGISLAEMIRQGKIDIISCTGANLEEDIMNLVAHSHYKRVPNYRDLTPEQE
                     WELLEQGLNRVTDTCIPEEEAFRKIQHHIAAIWKDADAKGERYFPHEYMYKLLLSGVM
                     QADYEIDPKNSWMIAAAEKNIPIIVPGWEDSTMGNIFASYCIKGEMKTSTMKSGIEYM
                     IYLSEWYRANSSGKGVGFFQIGGGIAGDFPICVVPMMYQDLEWHDVPFWSYFCQISDS
                     TTSYGSYSGAVPNEKITWGKLDIHTPKFIVESDATIVAPLIFAYILGW"
     gene            22786..24075
                     /locus_tag="PLL28_02995"
     CDS             22786..24075
                     /locus_tag="PLL28_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNF68311.1"
                     /translation="MASGLHFIPVEEALQCIKSGDRVFVHGSACTPTYLLKHLAERHN
                     ELRDVELVFISVYGDMHVDKPEYRDSFKLNSMFVSNSIRADVNEGYADYIPIFLSEIP
                     ELFKRDILTLDVAMVQVSPPDRHGYCSLGVSVDIARTAVNKAKYVIAQVNPQVPRTHG
                     ESLIHYSRFHCMVYADEPIHEAVFGEKVGPAELKIGEYIAGMIEDRSTLQMGIGAIPD
                     AVLKCLTNHKDLGVHTEMFSDGLVPLFHSDVVNNKYKKVHPNTTVSGFALGTRMLYDY
                     VDDNPAFEFLDIDYVNEPSVIKQNPKVVAINSCIEVDITGQVCSDSIGTYQYSGVGGQ
                     MDFIRGAALSEGGKPIIALSSRTAKGVSRLVPILKPGAGVVTTRAHVHYIVTEYGIAY
                     LFGKNLRQRAKALIEIAHPDDREMLHKAAFERFKIFV"
     gene            complement(24219..25160)
                     /locus_tag="PLL28_03000"
     CDS             complement(24219..25160)
                     /locus_tag="PLL28_03000"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HNF68312.1"
                     /translation="MKKMCTLIAAIAINAAVFAQTDSTSNWKKGGGAILNFNQTSLTN
                     WAAGGESNLSSTILTNFYANYKMGTTTLDNTLNMNYGLITANNYADIRKNEDRIELNS
                     KYGRYAFADDFYYSALVNFLTQFAPGYDYLADPDALVPISKIMSPGYLTAAAGLDWKP
                     GKGEKFSLFMSPATGKFTFVMDDTIASFEADTVNHKNRYGVTTGDNLRAEFGASLIAA
                     LNTSLGKNTSLSSKLTLFNNYTDPIADHRANIDVDLQNNLNIKVSKYIAATIFVQVLY
                     DHDIAIPTYDENDVQIGTGPKTQFKEVFGIGLSYKFE"
     gene            complement(25254..25667)
                     /gene="mscL"
                     /locus_tag="PLL28_03005"
     CDS             complement(25254..25667)
                     /gene="mscL"
                     /locus_tag="PLL28_03005"
                     /inference="COORDINATES: protein motif:HMM:NF001843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNF68313.1"
                     /translation="MSMLKEFKAFAMKGNLIDISVGFVMGAAFTAVSSAFIQGIVMPF
                     ISLIVQKDFSTWKLRLKAAEKAADGTVIKPEIAVLYGDFISAVIYFLIVAFVMFMVVK
                     AMNSMKKKEEEAPAAPPAPPADVVLLTEIRDLLKK"
     gene            complement(25764..26240)
                     /locus_tag="PLL28_03010"
     CDS             complement(25764..26240)
                     /locus_tag="PLL28_03010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF68314.1"
                     /translation="MEQLFWKIKSFHQLNISELYGILRLRQEVFAVEQNCVYLDVDGK
                     DQFCDHLFGVDDAGRVIACCRIVQPGTSYPEVSIGRVATHPEHRGAGYGKQVMDKAIS
                     FIEDRYGKVPVRIEAQYYLEKFYIDYGFEAVGDVYLLDGIEHREMVRNAVHAKARV"
     gene            complement(26421..>26815)
                     /locus_tag="PLL28_03015"
     CDS             complement(26421..>26815)
                     /locus_tag="PLL28_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68315.1"
                     /translation="IADLLNGIMEPGSIAMMLLILLPLNIFFAAVTLMLSIYARSFKE
                     AQSMITPLMIFIVFPTIFGMLPGVTLDYGTALVPILNVSLGAKEIISGTVEMGPLLLT
                     YLALFVYAGIALMVSVRFFNNEKNILRG"
BASE COUNT         7767 a         5221 c         5618 g         8009 t
ORIGIN      
        1 aggccttggc ggtaaaaaag tacgagatat ctggacacaa cttggcattg aaactgttgg
       61 tgaattactt tatgcttgct atgaaaacag attgtcgaag ttgaaaggtt ttggcgaaaa
      121 aacacaggca gcggttatac agagtattga atattatcag aataatttag gcaaattcca
      181 ctacgctgct gttgcggaat attgcgataa tatattaagg caaatacatg atctgatttc
      241 agataagcgc ataatgtttg tcggcgatct gcgcaggaaa tgtaatatta tcaatgcgat
      301 ggaaatcatt gcacctgcat cagctgacat ttcatctcat atatcaaaca taaatgagat
      361 agtggttgaa gaatcatcgc aatcgctaat aaaaggtaag acagcacaag atatgcaatt
      421 cagcatactt attgtttctg atgaagatta tgtgatgcaa ttgttccgtt tgacagggtg
      481 cgaggagcat gtgaacatga tattatcgct cgcggggcaa gggccatttg catcagaagc
      541 ggatatttat tcagcagcgg gattgccatt cgtattcccg gaaatgcgtg aacaatttgc
      601 agaggtagat ttaatgcaat ttttgcataa ggctacatcg cccaaacatc aattaattga
      661 attatctgat attaaaggag tagtgcacaa tcacagtacg tggagtgatg ggaaaaacac
      721 gttagaggaa atggcgaatg cctgcgtgga agccggttac gattatcttg taatttctga
      781 ccacagcaaa tctgcatttt acgccggcgg attaacagaa gcgcagatcg ttcagcagca
      841 tagtgagatt gataaattaa ataagcaatt tactgacttc catatattta aaagtatcga
      901 gtgtgatatt ttgaacgatg gcggcatgga ttacgacaac gatattttag caacatttga
      961 tttgatcatt gccagtgtgc atcagaattt gaagatggac attgaaaaag ctaccactcg
     1021 tttattgcgt gccatagaaa atccatacac ccgcatactt ggccatatga caggtagact
     1081 tttacttagc agaccaggtt accctgttca ccataaattg atcatcgatg cttgtgctgc
     1141 caatggtgta gctatcgaga tcaatgcaaa tccatataga ttggatatag actatagttg
     1201 gataccgtat gcagcagaca agggtgtgct catctccatt aaccccgatg cgcatagcgt
     1261 tgaaggtatt tgggacatcc gatggggtgt gcaatcggca cggaagggcg gacttgccgg
     1321 taatcaaacc ctcaatacca aaacgttgca agaatttcgc aattggttag agcgcaagta
     1381 agtttcagcg accaaatagc tgattgataa gggtttacct ctcggattgt cgtaactttg
     1441 tgttcgatga agaagtattt ctcattaatc acattcagtc acacggtatt tgctttgccc
     1501 tttgccctca ttggatttac attaggcata acgcaaccgg gcgttcaatt tgaatggttg
     1561 gatttagttt tcgtattgtt atgtatggtg tttgcacgga gtgcggcgat ggcatttaat
     1621 aggtatattg atgcagatat tgacaaggat aatattcgca ctgcctccag agagattcct
     1681 agtggaaaaa taagcaaatc gaaggcatta cagtttacaa taatcaattg tatcggtttt
     1741 gtaatatctt ccggcatgtt gaatgtattg tgtctttatc tttcgccaat tgccttgctc
     1801 gtaattttgg gatatagttt gaccaagcgg tttacatggc tttgtcattt tgttctcgga
     1861 atcggacttt cactggcacc aataggtgcg tacatcgcgg tgacagaaca ttttcaaaca
     1921 ttgcctatca tattttcgct tgcagtattt acctgggtat cgggttttga tattatttat
     1981 gccttgcagg atgaggaatt tgacaaggca cataaattat attcattacc tgcaaagctt
     2041 ggaaaattca atagcatcat agttagtata attgtgcatc tgaataccgt attattatta
     2101 tggtatgccg ggtggtatat cgaatttgga actatttatt ggataggcat gggcttattt
     2161 acaactgcat tggtatatca gcatttaatt gtaaaacctg atgacctgag tagggtgaat
     2221 cgcgcctttt tttctgccaa cgggattgca tcggtggtat ttggaatttt tgtcatcaca
     2281 gatctgttgg tataaatatg gcacgcattc ttggtattga ttatggcaca aagcgtgtgg
     2341 gccttgcagt aacagaccct atgcaaatca ttgcctctcc gctgacaacg gtagatacgc
     2401 cgcaggcatt agattatata gccaaataca ttgctgagaa tgaagtagaa aggatattga
     2461 ttggatatcc cacagattta caaggcagac caactgatgc cacttttcat gttgagggct
     2521 ttatcaaggc aatacaaaaa cgacttcctg aaatgccttt acagaaatgg gatgaatcgt
     2581 ataccagtcg catggccatg cagagtttgg tggcaagtgg aataaaaaag aaacagcgac
     2641 gtgataaaaa attattggat caagtgagcg ctgctataat tttgcaagaa tatttacaaa
     2701 acctttgata tgattttacc tattgttgca tacgggcatc cggtattaaa gcaggttgca
     2761 caaccgatca ctgccgatta tcctgatctg gcaaaattga tcgccaatat gtgggagacc
     2821 atgtacaatg gccgtggtgt aggacttgca gctccacaaa ttggcttgga tatcaggtta
     2881 tttgttgtag acagcgagca actgaaagat gatgatgacg aagaagatga tgatgtgcga
     2941 ttgacaaaag gtataaagaa agtattcatt aatgcccaga ttattgaaga aacgggtgag
     3001 ccttggagtt atgaggaggg atgcttaagt atccctgata tacgtgtgaa gatcatgcga
     3061 aatgaaactc tgcgcataaa atacgttgat gagcattttg ttgaacatat tgaaacatat
     3121 gatgaaatga atgcgcgcat cattcagcat gaatacgacc acattcaagg cgtattattg
     3181 acggatcaca tgacaccatt aaagcgcaat ctcatcaaga aaaaattgga caatatcgct
     3241 accggtaaaa tctctaccaa gtacaaaatg aagtttgcca aataagcaga cgatttatgg
     3301 tactttgatc attccttttt gaaatcgcca cgtttccagg cttcaaagaa ttccttccag
     3361 gcaaagacat cgaatatgcg cattggtgga tattgtcctg cgtaatagat cttttgttga
     3421 atagccttag tttgataatc ggcattcata tccgcatcca tagccattgc ttcgccgagg
     3481 tcctttaatt tttcacggtc gaggtttttg cgagctacct cgtaatcatc atcagggatg
     3541 tcaagggcaa gaaaagcatc gcgaaattcc tctttagatg gccaagggta gataatcgtt
     3601 tcggctacga aaatggtatc acgcgtaagg agttggatta cagaatatct tgtatctgtg
     3661 agatcacctg gaatagtgaa tacctgatct ttatatccga gtgctgatat gcgtatggag
     3721 tcacctttcg cagccacaaa ggagaaaaaa ccgttgtaat cgcaagttgt cccgttattt
     3781 cttgtcagcg accaaatacc ggcgtatggc acggccataa gagaatcgct ggtcattaca
     3841 agacctgaga attgaataag gctatcagtg gtagcaacct gtgcgcgcag ggagatacaa
     3901 gtgaacagtg agagtataga gatccatttg cgcatatagc ctaaacgttt tgagtaaaga
     3961 tacagtattg tctgcagagc ggtttcagac aacagacgcg ttcaggcact aaaagtttaa
     4021 ttgatgcgtg tgttattgtc ggtcagtgaa accatagcat taggatttgc tgttgagcca
     4081 cctttaagtg tagagaagga cacatccact gcaggagctt ctgttttaat tgaaggtgcg
     4141 tcatcatcac ggaaggtata agattcttct gtggcaacta cgaagccgat atgtgctgcc
     4201 agtatggctg ctagaacgaa aggaccgctg agctttttca taattggagt gctttgatag
     4261 gacgaaaata ccgccgtatt gaaggcgcag ccaagtacaa aaacgcaggg ggtatcatgt
     4321 cactttttgg gatctcaggg cattaaaatg aggagaaatg tcgattttat tgagttttaa
     4381 gaagtgttaa gtgatgaaaa gatgacaaaa attggcgctt atcggatggg gtagtggagg
     4441 gggtgaaatg aagctcagac ctctaatgaa atcgcgccaa aaaatgtcaa acctggcagg
     4501 tttaagacct aacaggtcta agacctgtca caacccttgt agcactgagg tttcaagtcg
     4561 agattgtatg ttttaggccg attttttctt tgtattcaga tatatgtttt tcacacttaa
     4621 atggctatca gacctctaat aataaatgat aggattgttg gtcttttata ggtcttagac
     4681 ctaacaggtt taagtcctcg cataacccca tttgggccgg ggcttctctc aaatccacca
     4741 aaaacaaaaa acctctccgg ctgtcggaga ggcttttgct tgatttaacc cctaatgttt
     4801 aatcagaaag tttataatac cttgatcttt ttaacaactt tattcgcccc attctcaatg
     4861 ctcacgaagt atataccttc tttgagctca ctgatattaa gcgacatcac cggtgaagta
     4921 attgccactt gcatcatatc ctgacctaac aggttgagca cgtgaattgt tccgtctgac
     4981 aatccggaat taacataaat ataatctgtt gccggattag gataaacctg cgcttcttcc
     5041 aatgaattgg ttccttcaag cttgatattg tcgccggtat aacttccggt atgactagct
     5101 ccttcatctc ccacttcggt attgccgttt tctaatgaac cggtagatac ctcagttgca
     5161 actgatggat taagatcgtt tatatccggc tgcaaaccgc taagattgtc gatttcaggc
     5221 agattggtgt cctccgaacg ttcacgtgca ttggtttgcg caaacatcac cgctgaggtg
     5281 agcaggaagg caagagttat aagaattctg ttcatggttg ggtggtcaaa tatacttaag
     5341 taatacgggc ttgatcctat tttgttttaa aattatcggc acatcttcac ttacgttaaa
     5401 gacaaaaaca cagttttatc ggaacccttt ttctcaaata tatttatttt atatacttaa
     5461 cttattgata atcaatactt tgttattcat ccacccccta attttgcccc tttgtatcgg
     5521 agaaaaatcc tgtggaagga ctgtgaactg agttactatt gttaaattta accgcttttc
     5581 gcgatgtttc aaaataaatc tatcgtcatt ttaagaacat ttacgaacca ggaggtccgc
     5641 cagttcgatg agtacctccg ttccccgttc ttcaacaaga atcagaccat agtgcaggct
     5701 tttgatatca tcaagtcttg gcatccgcta tatgatcatc ctaatctgga gcggacgcaa
     5761 ttgttcaaac tcatcttccc gaacgatacc ttcgaagagc aaaagttgag atacctgatg
     5821 acagatctca cgaagcatct ggaagaatat ttgtgctata aggcgatttc tgatgagaat
     5881 attttcaaat atcatctact actgtatacc tatcgcattc gtcgccttga caaggcattt
     5941 ttgaatacac ttaaacttgc tgaaaaggca attaaggatc aacctcgtcg cgatgtgtcg
     6001 catgcgtttt acgaatatct gattgaagaa gacagatacc gtttcatgag cgacaggcgc
     6061 gagcatttac atgaaacgaa cctacagcag gtagtagata atctggataa atactatatc
     6121 ctcaataaaa tgcgatatag tgcagagatc atcaacaata agaatgtggt tgcaatcaat
     6181 taccgcttat tcttgtatga tgagatcatg aatcatatca ggcataatcc gcttgattat
     6241 gttcctgctg caaaaattta ttacaacatc attctcacac tcaccgagcc tgaaaataaa
     6301 attcactaca cgaatctcct cgacctgtta aaagaacatc atcaattatt ttccaaagag
     6361 gagatttttg aaatgtatgt ttatgccaaa aactattgta tcagaaaaat caaccatggc
     6421 gatatagaat acattcgtga actgtttgat ctatacaaag tgatgttgaa agatcgtatc
     6481 atttttaagg ataattattt gtcgcagttc gattataaga atatcgttta cctgggattg
     6541 cgcattagcg agtttgactg ggttcagcaa ttcattgtga aatacaatga gcatcttgat
     6601 cctgcaatca gagcgaatgc ctatacttat aatatggctt atttccactt cttccaaaac
     6661 aattatgatg aagtgcttac gctccttcgg tcagtcgaat tcaccgactt ttattatcac
     6721 ctcgattcga aatcattgct gctcaaaact tattttgagt tggaggagtc agaagcattt
     6781 tttagtttga ttgaggcctt taaagtatat attaaacgga ataatctgat ccctgcttat
     6841 cagaaatccg gctatacaaa catgatcaag ctaacgtcta aaatgtttaa gtggaaactc
     6901 aatccccgca acgatttgca agatcttgtc gatgagttca ataatacaaa gcctgtcgta
     6961 gatgtattat ggattaaaaa gaagcttgaa gatttgcaaa atagatctga aaaattgtcg
     7021 ggaagctgga gaaagtaatc ttcacaaacc tgcttgctaa tttttacttt tatttgcaat
     7081 ccgttcttaa tgttcaacga tttgattgga attttgtcgc caccaattaa tcagctcttg
     7141 ctttttatcc tgcaatgcaa ttgagttaat atcctctgca taatactcag gccacaagga
     7201 tggatacata ttgttcctat atccttcaac catacgcatg gcttcaatgc caatggttgt
     7261 atgcattccg ggcttaggtg caaaatctgc gtaaactgaa aatacaggaa tggttaatgt
     7321 attgtcgtta aatttaatta ttaggctatc gatgtgttcc ggtttgatcc aattctttgg
     7381 caccttttta atagcagtga aatcaatatt gttgtctgta acgccatttg gatataatcc
     7441 aacgtacaat atatttgatt tctcgtcact taatgcttgc aaaaattgta gtggcgacaa
     7501 ttcttcagga tctaaatcag acgttttata gaccggatat tgtttataga tttcagcgga
     7561 ctttttatct gctgaattat tgcatgcttg ccaagaaagt atagtaaaaa gaaaaacccc
     7621 gtttagtaaa acagggtttt tcaataaata tctattgatg ttattctttt ttagcatctg
     7681 tttcaccttc attcacgcgt ttagcaagat ttttcatcaa ctcgattcca agcaatccgc
     7741 taatcgggcc attggcgctg tcgccgctac cggtgatgag tacatcagga ataactttga
     7801 ttttatttac accgatgttt tcggtgatct tcaattgagt aaagttatcg ccacccattg
     7861 cttcaactga aagcttatat gattcggcat tcgatttacc aatcgcaaga attttttcag
     7921 cttctgcatt accggttttg ctgatgcgtt cagcttcagc gtcggcgagc aattttactt
     7981 tttcaccttc agcatttgcc aatacgcgta ttttttcagc ctcaccattt gcgagtaatt
     8041 tcaaacgatc agattcagca gtagactgaa tgcggatacc atttgcatca cctgtagctt
     8101 ttttcacact ggcatcggcg atcttttcgg caatcacaac accctggtca gcttttacca
     8161 gttccttctg catatcggca acagcagttt ctttttccag cgtgcgtcga gtttcctgcg
     8221 ccttcatttg agtgtcgtaa gtcacttttt gttcttcggc aagcttacga tcagtaagtg
     8281 ttttcatcaa tgattcaggc ggaatgatat caccgatcaa agtatcaaca ccgtttacgt
     8341 tgtattgctc aagcactttg ccgatatgtt gtttcgcact ttcctggcgc tctttacgca
     8401 ttttcaggaa gtcaattacg tcggcatcct gcgctgagtt acggaagtag ttaccaattg
     8461 taggttcaag aacctgtgta acaaggttgg ccatatttcc aaatcgtgca attactttag
     8521 gcgcctcata tgttggaata tggatgattt gcgatacatc gaggttgaag gtaaaaccgt
     8581 cttttgaacg aaccgtgatg gtgctcaaat ttttgtctaa ctgatgcgat tccgtgcgtg
     8641 cacttgccca gtttaacacg agatttgtgg taggcactaa ttcaaccttc ataatatatg
     8701 ggttgatagg atatttaccg ggacctaaag catctgccca aacacctttc tgaccttttg
     8761 aaacgatatt accatgttta aattctgttc cactgagatc aacaccttcc ggaccaacat
     8821 aactaattac aacaccaaca taaccaattg gaatttccgt cattttaact atctccatct
     8881 ttacaaacca tgggtttaag aagtagctac cggcaagtat tacctgctct tgcaaacctt
     8941 ttaaaccggc atttattaag aagccatcag cgtcttgaaa gcggttatga ccttctacaa
     9001 tcttacctgc aatctgacca tcatcaagtg gttgaccttc aagtgttgtt acaattccta
     9061 cacagttatc gggaatattt gtcatactca cgatctccac atcgaaaagg aaggtattga
     9121 tacgataaga tccgggtgcg ataatagctg tttggcgacc tttacgaccg ccgccatcca
     9181 taaacgcgat agcatcctgg aatgaatcgc attctacttt tctggccaat acgcgaccag
     9241 tttccagttc aacgccatct tttgccatca ccaagccgat ttgaccggtg ggaatgatga
     9301 tgaatggctg catagtgatt tcatactgcc agatccattt ccagaaataa agacccggcg
     9361 caagactgtg tgcctgaaag ccggcttcac ctttcgtggc gataatgtga ccatcaggaa
     9421 gctctttgtt agcgccgaag aggacgaatt ttttggttac aagtccgatc ctgtcctcag
     9481 gaacgatgac cataccaaag aaaacgcgca aaataaattt gtagaaaacg agtgcgagga
     9541 taatgccaac gattatcatc gtaagtttaa gtgtttccgg gctcatagtg tccaatttgt
     9601 tttagtgtga ttaacagcta taaaagacgt aaaggtaggg aagaaatttt caggtattgc
     9661 attatttttt tgaagcatgt aattagatga aaatactcaa taatcacgct catttagtgc
     9721 taaaaagctt gattgtataa tctagtagcc tttgatcgcc ataatcacca tgtccgggaa
     9781 cgacaatttg cacctcgggg aatgcagttt ttacatgatg aacggtttca ggccaagcgg
     9841 cggtattggc atcggcaagg tatccctttc ctgcatctat agacttgatg agacagccac
     9901 caaaaaggat gttttccgca gggaaatatg ctactacatt atctcgggtg tgcccttcac
     9961 cgaaaaattt cacaatgatc tttttgtcac ctatcggtaa ttcaagtgaa tcggaaaagc
    10021 tatgttcagg aattgtaata ttattttgct tagcaaattc aatggtcctt gtataagcat
    10081 aagtaggaat atgttgctca tgaaaagcgt ttaatccgcc aatacaatca tcatgaaaat
    10141 gtgttggaat gatggcatta attgtaacat ccaagttttg ttttacccaa tttatcaaca
    10201 aggttgcgca agagtcattc acaggagtat ctaaaataat acattccgat ttgtcggtaa
    10261 tgattacacc attgcacgga acaagcccaa aatcatcggt ttgtttatac gaagtatgga
    10321 tgtaggcatg ttttgaaatt tgggtaatgc gcaaggcatc agtcttatat acttcaacag
    10381 gtttaaatgc aacttttccg gtggtgctgc actgaaagcc tgctaacaaa cagataacaa
    10441 ataatgtcgt tacaaataag ttgatgcgtt tcaatccttt aattattatg atgaagtggt
    10501 attaaaggtg tgaagttagt tagtaaatga tatataattt cagtttttga ggtgaaataa
    10561 ttttcggtaa tgattgccat tcccaataat taaaccttac atttacagaa atagggattc
    10621 atgaaaatgc tattatgcag tttattgctt gggatttcaa ttaatttgcc ggctcaggat
    10681 gcatcgatat tgcaggagaa atggtcatat catttaatca atcaaaattt ttcgagacca
    10741 acctgtgcag ttgaatttcc gaatgggcgc attatggtgg gtatttacca ttctatgatg
    10801 aataactata atgaattctc taccatcctc gtcttagata gtgcaggcaa taaaatcaga
    10861 agtgtagtcc taagtgatac catgaataac tatgtgtatg atatgtctct tgcctccgat
    10921 actacagcta ttattgtagg aaaagcgggg ccgcttggac agaatggaac aatacccgga
    10981 gacatatata tcgccaagct gaatgtttcc ggagatatta tctggagtag gaaatatggt
    11041 agcagcgatg ccgatgaggc taaagccgtg atacccgatg gacaaggagg tgcatatgta
    11101 tgcggtcgag taggctttgg tgatggtgat gtgtccacct attatggtaa tcgtaattct
    11161 gctgatatat ggacattgca tattgatgca tcaggaaata tactttggca gcaaacgatg
    11221 ggtggcagtt catatgacag accatacgct atgctgaaac tcgctgatgg tggattactg
    11281 atattaggag aatcggcatc gtatgatggc aacatcgaat cgtgcgaagg tggatttgat
    11341 gccttaatgt ttaaattgga tacaatagga aatcttgttt ggaaatatag cagaggcact
    11401 aaatacaatg atacgtttat tgccgccatg gtaactgatt ctttaattac agctcaactt
    11461 gttcaaagtg aatatgtggt gagaacaggt aaaacaaaaa gggaggaatt tacaattaca
    11521 atggatcaac atggtgaggt tttatcacaa cgtaaatccg atccggatat gatctttgaa
    11581 aatggcgagg cgtattatta tttcaaaaag gatcctgatg atgaaaactc aacacgttgc
    11641 ctcaccgtag ctgatgctga gcctcgtcgc acgtattact tcccttggga ggcacaaaca
    11701 caactcgagg aggttttgca acttcataac ggcgacctcc tgttattggt aggcgtttat
    11761 gtcaggacac tcaatgcgtt catagttcga attgaaaact gattggattt atttcccttt
    11821 ttaccaatta attgttatta ttcatctgtt aagatgcgat tgttcacata cttattcaaa
    11881 gattctatgt ttgatgcgca tttgaatcgt cgcgcttgat ttttttgtca ggtgcacttt
    11941 tgtatctccg cgtgagtaat aaacagatat acaaattgtt aattgaattc cctgctatgt
    12001 caggtatcag ttatttcttt aaaaaatacc ccgcaaaatg attgtttgtt gttgttattt
    12061 gcaacatata cattgcagga ggtaattgac tgatgctaat tacctcacct gtccatgtgg
    12121 aggtctgata tactatttct ccacgaagat tcacgatgtt cagcgttgaa acttcattgg
    12181 atgcgacatc aacaaatatg atgtcggatg caggaatagg gtaaataatc aattgttctg
    12241 aattattgct attcatacca agatcctcat cactgaaccc atcgcaatca tcatccaaac
    12301 cattcaacaa ttccgtcgca ccgggataaa tattcggatc gatatcattg caatcagtcg
    12361 aatcatatac atatcccggt atatccatac aagctaatgt gtcaatagac cagttgccat
    12421 aaagatctcc atccaaatcc tgaaactgat ttagaaaagt cagattttca tccacaaaac
    12481 catcgcaatc gtcatccaat ccattacaaa tatcctcagc tcccgagaaa atactcatgt
    12541 tagtatcgtt acagtcgagt gcgttggaga caaatccgaa gacggtcatc acacaagtgt
    12601 caataacaaa tgcaggatca ccgtatccat caccgtcgtt gtcgacaaat aaagtataca
    12661 cggtcagatc atcatccact agggtattgc agttgtcgtc tatgccgttg caagtctcac
    12721 tttgcgcagg attgatttct gtatttagat cgtcacaatc aagattattg taaacaaaac
    12781 caataatatc gctgcaggta agtgtgtcga ggtcagcatc gccatagcca tcatcatcca
    12841 tatcggcata caagaaatag gtgaccaatc cttcatctgc aataccgtcg caatcgtcgt
    12901 ctaaagcatt gcagatttca gaggatagtg gataaatcaa gaggttcccg tcattacaat
    12961 cgagattatt ttcaacaaac ccttcaggtg catcgcatgc aagtgtatct aaagcgtcgt
    13021 caccatatcc atcgccatca gtatctgcat agaaatgaaa tttagggcat acgatattgg
    13081 tggtagcaaa atttgtataa acaggatcat tgaaatcaaa cacgatgcct gcataatttg
    13141 aaatgctgtc gccaatggtt aaagtatgat caggactcaa tgagtattgg atgtatccat
    13201 gactgccagg ttcgttagtg gtgctgtcgg ggagtaaaat gctgttgaaa gtgaatttta
    13261 gtaagcgtga atctgcgtaa tattctaaat cacttaaaaa gtgggatgtt gtgatcagtt
    13321 gaaaagtgct gatatcagtt ccgaccggta aaatattatt tacaatgata ttgaaagctg
    13381 tatccgtacc aatattttga aaattgatgg tgtaattaag atgatcggtg acagcaagtt
    13441 cttcaggggt gagttcaacc agattcacga tcatattatt cggatcatag gaagcaatta
    13501 tttcgtttac actatttgaa atactattgt caactatgct gtaaacatcc ggtgaataaa
    13561 taagcgctga agatgtcacc atgtcgcccg catcagcagt ttcactgacg ataaaagtta
    13621 ctgttatgag tccactttcg aagggagcaa tggttcctaa cgaccatgta atggaatcat
    13681 cgtataatgt atatcctgcc aggtcggcac tcataaacga caacaaggga tcgtatatta
    13741 aattgatttg accattaata gatgttgtgc ctatgttctg ataggagagg gtatatttta
    13801 cagttgcacc gggacgaaac aaataaattg gagtgatatc gatcaccaga gcattcatta
    13861 cgctgtcggg ttgaaatcca aagttcctca aggaatcaac cgtataaaaa ctatcgatca
    13921 ctgttgtata attggccgga acagaagtat aatacggcag cgtgtcagaa ctgtaggaga
    13981 aggtatccaa acttgttact tgaaatgcat aatatcctga tgaatcactg aattcagtca
    14041 aaccttcggg agaaatatat acgggcttgt aggttaatgg tggttcccct gcgtcatgca
    14101 gtgtattggc attcaggtcg gcatataagg tgccggagat ggtagaaaaa tccggtgtaa
    14161 gctttatcag ccataaatca taattgccca agttgggagc tgtcttattt cctgtgacta
    14221 ttgatctgga agaaccggat aataatacgc cgccatcatt ggtgattgtc atggatgtaa
    14281 ggtcatcgtc gtttgtacca ccgaaaacct tttgccagag gatatttcct gattcatcag
    14341 ttttgactat ccaataatcg taatcaccat aggtgtttga atttttgtca taacctatgg
    14401 atgatgcaga tttgccaccc aacatatatc cgccatcctg tgcttggata atacaccgta
    14461 aataatcaag gttattacct tggattgtat tttcccatac cacttcaccg gtagcattaa
    14521 tcttcaccac ccagtagtca tagtaataaa ttgaattttc agatttatcg taccctgcgg
    14581 gcgactgtga ttcagcggca agtaaatatc cgccatccgc agttttgatt gcatccacac
    14641 aattgtcaaa atgcgttccg ccgatcacat tttgccattc gatatcaccg atactattaa
    14701 tctttatgat ccaaaaatcg gtgacatcaa tcatgggctc agttttatcg cctgtgatat
    14761 cagaattgct gtaaccgcat aataagtaac caccatcatc tgattggata gctctcacta
    14821 gttgatcgtc ccaaaaagcg taatattcgc gctgccattc aatagttcct atattattta
    14881 atttgtagac attgtaatta tatataaaac tacctgcctg ttccttgttt gcacctaaaa
    14941 tataaccgcc atcaggagtt tggttgatac tgccaacaaa gtcgtcataa ccaccaccaa
    15001 atactttctg ccattcgata tgtccggtgc tattgagctt aaccacccag gcatccggat
    15061 ttccgtacgg acctatcgta ttggatgtct taacaccgga tatttcggaa ttagaagttc
    15121 caccaagtat atagccgccg tcgtcagtga gtgataaata gttcaggtag tcatcgccgg
    15181 aaccaccgat ggttgtttcc cattcgacat tggcagcgga atctgtttta ataatccaat
    15241 agtcggcacc cccatagcta ggctctgatt tatatgcaga aatacctgaa agcgagctgg
    15301 agccaaaaat aaatccatgg tccggggtca ttaatacgat atctgctgtt tcgtaatctg
    15361 tgccgccaat tgttttctgc caatccatgg ccaatgactg accatacagg ctgccacttg
    15421 taactagcgc aagaagaaat agggatttga ggtataaagt tttcatggaa atgggagttg
    15481 tatgcaaatt tagggaaatg tagggaaagg agggaataat taggggatag aaatgtagga
    15541 gtgtggaagt ctgcaagtgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
    15661 gcaagtgtgc aggtttgcaa gtgaaataca gatgtgttca tttgcttgtg tgttctggtt
    15721 ggaaatttaa gtacgttgct gaaggtattt cgccccctta ctctccgaac ttactatgaa
    15781 gaatacttgt ggatggcttc gtaatccttt tgtactttgc cgtcgataaa acgattttgt
    15841 atatttattg atgttctttt aagggagaca tggttgaaag taaataaata taattctcga
    15901 aagtgctatg gttttacgcc aaataaccga gcgtacggct tcacagcttt tttaataaat
    15961 cggaatactg ctggtggtgg acctaccggt attgaatcaa ataattcatc tagcacgcat
    16021 attttagcaa acatcttcaa agtaaggttt ttgcttgtca tcagtggata caattgatcc
    16081 aatgaataag caaattcaac gggtatatga tcttccggga gctgtgggcg gaggactact
    16141 tccttgtcag tctgattgaa tatcttatgg aaaacgccgg cagggagatt tacacgttca
    16201 ccttcgtgta tttgcacaac ttcgccatta attttcgcac ttaaagttcc ttgtaccact
    16261 tgaatggtct caggcatttg ttcatgcata tgttccggcg gacccggtgc aaaaggcgca
    16321 aatcttaact cgcaatatac tttattatcc tcctggcgta tgatcgtttg tgttatccct
    16381 tctcccttag acgaatatgt ttgtccgggt ttaaagtagt tagaaagatc gggttttttt
    16441 aaaggaaaaa atacattata aacgaccggg agcattatta tgcccaaggc aatgattaaa
    16501 attatcataa tgttatgagt tttgttggct tgtcaaaaat aatcaataag atttcctatc
    16561 tgctaataaa ttattttata cctgtagcgt aaacattatc tgctctagtg cctcgcgtcg
    16621 aatatggacc atcgtagata attgaaattg gtaagcaata cccttttccg attacatcca
    16681 aacagctcct tataaacact tccccgatca cgtagatttt ttgatcaaat atggctatgt
    16741 atgaattaac ttttaatcgg aataatttgc catgcatcat ctttcgatga ccgcaacgca
    16801 tgatgaccga caggaaggat ttccaattcg agaagtgtgg ggtctaattc taatgcctca
    16861 aaatatgtta ttattttagc attagccatt gtatgcgctt gtattccaca cataaattgc
    16921 cagtcgccat catggtcgtg ggataccaaa agtataggga gttccctatt caatacatga
    16981 tcgcaagtaa aacacgcggt ttgcggggac tcgggttcaa atataatttc catgttatgg
    17041 taaaggtctt taaattagca taagcttgca agtggaatgc agttgtgttc atgtgctatt
    17101 gagttcgggt gtttttgtgc gaaatgctac gtgttcatgt ggttgtgtgt acaggctctt
    17161 tgcagccgaa aataaccagt acccaaactg tataccagaa tgccgaaaat gcgtatattg
    17221 gttttgtcac cgggggcttt ttaaatagtt tttttgggga agtaaatacc gattaaaata
    17281 gttagtttgt gcatacaaat tgactccaag attaaattta ttttcgagac gcgtatttct
    17341 ccatttcttt ttctatgtcg gatttaaact gctgtaattt aatttcattt tcaggaccta
    17401 aatccagcga tgctctgcca accggaattg gttcatttac tcgtggggca gaaattaagg
    17461 tgtcatttac tattacagca atttttttac cttgggcttc ttttgttccg gcaaaccata
    17521 ggtctgttcc cattttgtcg aattggagtg acaatacctt atgaccataa taccctttag
    17581 ccatttctat tgacttaaag tgctttactg aaataacggg agtgggaata acatagtatg
    17641 tggcagtatc atataattgt gtgcgcttta ggccttcatt attgttgctt attctgtaaa
    17701 atcctgtgat caggaaggta tcttgaatga catattccgg ttcggtgttt tcgatatgtt
    17761 ttgatgcatt gctaaagcca ctgcaggaag ataataagca tccaatgaat aacaggagaa
    17821 gggcattttt catgagatga aaatagctgt aatgttttat agataaaagg atatttggat
    17881 ttttgtataa acatgcgacc ccttcggggt cgattgatgt tgtgtgtggt tctggggtat
    17941 aaatgtgcga cccctacggg gtcgattgat gttgtgtgtg gttctggggt ataaatgtgt
    18001 gaccccttcg gggtcgattg atgttgtgtg tggttctggg gtataaatgt gtgaccccta
    18061 cggggtcgat tgatgtttcg cggtaatttt ttttgatttg attgaaccta aaaggttcaa
    18121 aagaatttgc aacaatctca ttttcttgct acccctcaag cttactgcct atcaatcaat
    18181 aaagaaccac ctcccccaaa tcccgaaggg attgcctgtt aatacattcc ccatgcatgt
    18241 aaccccccga ccccgaaggg gtcgcatatt tatggccccg gtggattgaa atagcattgt
    18301 gtatctgaga tcatcgacag cgcatgccat gatgccactt catcttcatt ttattgcgtt
    18361 aggatgcatg cttttctagc cccgattgca gcgaaaaccc cacaggcacg accgcaggga
    18421 gtgccgagga gttgcagcga aaagcgggtg aggagctccg ggcggactat gaatgttgtg
    18481 ctcctgaata gagcgtttat ttcataaagt gttgcagaga ttgcgcataa tcggttagtt
    18541 ggctgattgg ctgattgatt tatgtgaaca tcctgtttac agtgttgttc tttggcctaa
    18601 agccttgtct ataaatcgta actttgtgcc ctaattcaat tgttgccagt gaaacttcgc
    18661 ataaacacct ccgaaacctt tgcccagcgc catattgggc caaattctaa tgacgtgcag
    18721 gctatgttgc aggctattgg ggctgattct atcgacagcc ttgtgaataa gaccgtaccg
    18781 gaaaatattc gcctgaagcg cccgctcaat atcagcgcgc ctttgagtga atataactac
    18841 ctcaaacatc tgcgtaatct ggcttcgaaa aataaggttt ttcgcaccta tatcggcatg
    18901 ggttattata ataccataac acccagtgta atccgccgca atatttttga aaatccgggt
    18961 tggtatacgc aatatacgcc gtatcaggcc gaaattgctc aaggtcgcct cgaggcgctg
    19021 ctgaacttcc agactatggt gagcgacctt accggtttcg agatagctaa tgcgagtctg
    19081 ctggatgaag gaacggccgc tgctgaagcg atgatcatgt tcttccatca taagaacaaa
    19141 aaggatgtaa catcacccaa gttctttgtg agcgagcttt gcttcccgca aacaattgag
    19201 atcatccggg caaaagccga accacttggt atagatgtag ttgtgggtga tcacagaaat
    19261 gcgcaaatcg acgataccta cttcgctgcc ttgatgcagt atcctgccgg aaatggtatg
    19321 gtgttcgatt atacttcatt catcgcccat ttacatacgc acaacgtttc ttcaattgtt
    19381 gccgctgata tattgtcgtt ggcagtattg caaacgcctg ctgccatggg tgccgattgt
    19441 gctgttggca atacacaacg ctttggtgta ccgatgggat ttggtggtcc gcatgccgca
    19501 tattttgcta ctaaagatgc cttcaaacgc gatattcccg gtcgtatcat cggtgtgagc
    19561 gttgatcgcg atggtcgccc cgcattgcgc atggcattgc agacacgtga gcaacatatc
    19621 cgtcgcgaaa aagcgacctc taatatttgt acggctcaag ccttgctggc tattatggcc
    19681 ggtatgtatg ctgtttatca tggtgcagat ggcatcagac aaattgcgga gaaagtgcat
    19741 ggacttacaa ctgtgttgga taacgggttg aagcaacttg gctttacaca atcaaatgca
    19801 caatattttg acaccgttca tgttgcctgt ggttcattga gtggcctcgt gcatcagcat
    19861 gccattgcaa agcacatgaa tttctactat catcaaaacg gcgatgttca aataacactc
    19921 gatgaaacta ccgaattgag cgacgtgcaa gatattttat ctgtatttgc agaggctaag
    19981 ggaggtgcaa ctgttgcatt gccatcgatt gatagtttgc aatcatcgtt gccggcttct
    20041 tcattacgcg aagatgctta tttacaacat ccgattttta atacttttca cagtgagagt
    20101 gaaatgatgc gctatatcaa gcgtttagaa aataaagatc tttcattaac aacttcgatg
    20161 atccctttag gcagttgtac gatgaaatta aatgcagcat cagaattatt gcctataaca
    20221 tggcctgaat tttcagcgat acatcctttt gttcccgctg aacaggtgga aggatattta
    20281 catatcataa aagaacttga acaggacctt tgcgagatca cacgtttcga cggatgcagt
    20341 ctgcaaccaa attccggagc acagggcgaa tatgcagggt tgatggtgat acgtgcttat
    20401 cacattcatc acaatcaatc gcatagaaat aaagtgctca ttcctgcaag tgcacatggc
    20461 accaatcctg ccagtgctgt gatggcgggt atggatgtga tcgtagtaaa aagcgatgcc
    20521 gaaggtcata ttgatatgga tgatttgaat gcacaggctg aattacataa agacgatctt
    20581 gcttgtttga tggttaccta tccaagtacg catggtgtat ttgaagaaac aatcatgcag
    20641 atatgtaata tcgttcatca acatggtgga ttggtgtaca tggacggcgc gaatatgaat
    20701 gctcaggtgg gattgacatc acctgcaaaa attggtgctg atgtatgtca cttgaatctg
    20761 cataaaacat ttgccattcc acacggaggt ggaggtcccg gcatgggtcc aatttgcgta
    20821 aatgaaaaat tggttccttt cttacccgga catccattat ttaaaaccgg tggcattaat
    20881 gctatcaagg ccattaatgc agcacctttt ggaagcgcaa gtattctgct gatcagctac
    20941 gcttatatta aaatgttggg tggagaaggc tgcacgaatg ctaccaaata tgctattctg
    21001 aatgcaaatt atctgaagag caaattagat aaagcttacg atgtgttata tctcggaaaa
    21061 agtggatttg tagcacacga atttatcatt gatttccgca aatggaagca tactatcgga
    21121 ttggaagtag aagatgttgc aaagcgtttg atggattatg gtttccacgc accaacggtt
    21181 tcattccctg ttgccggcac attgatgatt gagccaactg aaagtgaaag caaggaagaa
    21241 cttgatcgct ttgttgaagc aatgttgggt atcaggtctg agattgccgc attggaaaac
    21301 ggtactgcag ataaattgga caatgtccta aaaaatgcac ctcatactat tcgcgaagtg
    21361 ttgagtgaac attggactca tggttattcc cgtgatgctg ctgctttccc tgcatcgtat
    21421 atacgcgagc gcaaattctg gccttctgtt gcccgtatca ataatacaca nnnnnnnnnn
    21481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaactttgct gtaactgcgt tatagttgga
    21601 cgggattttt tgcgcaaatc tttgggacac ccgggcgcac tacatcaaac acaacttgta
    21661 agaaaaaatg agttgattat taatatattt gaaacgcata aaacaaagca atgaacaagg
    21721 gtccaatatc acaatttatt acgcatcatt accgtcactt taatgccgct gctttagtag
    21781 atgcagcaaa aggttacgaa acccatttac ttgaaggcgg caaaatgatg gtaacactgg
    21841 cgggtgccat gagtaccgct gaactcggta tttctcttgc ggagatgata cgccagggta
    21901 aaattgacat catcagctgc accggtgcca accttgaaga ggacatcatg aacctggtag
    21961 cgcattctca ctacaaacgc gttcctaatt atcgcgacct gacacctgag caggagtggg
    22021 aattactgga acaaggctta aaccgcgtaa cagatacttg tattcctgaa gaagaagcat
    22081 tccgcaaaat tcaacatcat attgctgcaa tatggaaaga tgctgatgca aagggcgagc
    22141 gttattttcc gcatgagtat atgtacaaat tattgttgag cggtgtgatg caagcagatt
    22201 atgagatcga tcccaaaaat tcatggatga ttgctgctgc agaaaaaaat attccaatca
    22261 tcgttccggg atgggaagat agcaccatgg gaaatatttt tgcttcctat tgcattaagg
    22321 gtgaaatgaa aacatccact atgaaatccg gtatcgaata catgatatat ttgagcgaat
    22381 ggtatcgcgc caattcatcc ggaaagggtg taggcttctt ccagatcggt ggtggtattg
    22441 ccggtgattt cccgatttgt gtggtgccga tgatgtatca ggatctcgaa tggcatgatg
    22501 ttccattctg gagctacttc tgtcaaatca gcgacagcac gacatcctac ggttcttatt
    22561 caggagccgt gccaaatgaa aagataactt gggggaaact tgatatacat acgcctaagt
    22621 ttatagttga aagcgatgcc accatagtag caccattaat ttttgcatat attttaggat
    22681 ggtgatttta atcaatcata tccaattaac ttccatctaa ttttgcatca ccggtttgct
    22741 ttatgcatgc cggtgatttt tttttgattt ttaaaagata atattatggc aagcggatta
    22801 catttcattc ctgtggagga ggccttacag tgcattaaat ccggcgatcg tgtatttgtt
    22861 catggcagtg catgcactcc aacgtattta ttaaagcacc tggcagagcg acacaatgag
    22921 ttgcgcgatg tagagctcgt tttcatcagt gtttatggcg atatgcatgt cgacaaacct
    22981 gaataccgcg atagcttcaa actcaattcc atgtttgtga gcaacagtat tcgtgccgat
    23041 gtgaatgaag gttatgctga ttacattcct atttttctga gtgaaatccc ggaattattc
    23101 aagcgtgata ttctaacact ggatgtagca atggtacaag tatcacctcc tgatcgtcat
    23161 ggatattgtt ccttgggcgt tagtgtcgac atagcacgca ctgctgtaaa caaggctaaa
    23221 tatgtgattg cacaagtgaa tccacaggtt cctcgtacgc atggtgaaag cctcatacat
    23281 tactcgcgtt tccattgtat ggtatatgca gatgaaccta ttcacgaagc tgtttttgga
    23341 gaaaaagttg gacctgccga gttaaagatt ggtgaatata ttgccggaat gatcgaggat
    23401 cgcagtacct tgcaaatggg tattggtgct attcctgatg cagtattaaa atgtcttact
    23461 aaccacaaag accttggcgt ccatactgaa atgttcagcg atgggttggt gccgttattt
    23521 cattcagatg tggtgaataa taaatataaa aaagtgcatc cgaatacaac agtcagcggt
    23581 ttcgcattgg gcacaaggat gttatatgat tatgttgatg acaatccggc cttcgaattt
    23641 ttagatattg actatgtgaa cgaaccaagc gttatcaaac aaaatcccaa agttgttgcc
    23701 attaattctt gcatcgaagt ggacatcacc ggacaggtat gctcagattc aattggcacc
    23761 tatcaatata gtggagtagg gggacaaatg gatttcattc gcggtgctgc tttgagtgaa
    23821 ggtggtaaac ctattattgc cctgtcatcg cgcactgcaa aaggcgtttc ccgtttagtg
    23881 ccaattttaa aaccgggagc aggtgttgtt acaaccagag cgcatgtgca ttatatcgta
    23941 actgaatatg gtatagccta tctcttcgga aaaaatcttc gccaacgcgc caaagccctc
    24001 atcgagatcg ctcaccctga tgacagggag atgttgcata aagcggcttt tgagcggttt
    24061 aagatttttg tataaatgtt tgggtacgcg gcaaacttcg cgcccttgca acttagcgtc
    24121 ttcgcgagaa aaacgcacat accataaaca gtaaaaaccg tataaaaaaa cctgctaata
    24181 atcatcagca ggtttttttc attaagctat taatttggtt attcaaattt gtatgacaag
    24241 ccgatgccaa acacttcttt aaattgtgtt ttagggccgg ttccgatttg gacatcgttt
    24301 tcatcgtaag ttggaatggc aatatcatga tcatacaaca cctgcacgaa aatggttgca
    24361 gcaatatatt tactcacttt aatattaaga ttattttgga ggtcaacatc gatgtttgca
    24421 cgatgatcgg caattggatc ggtgtagttg ttaaataagg taagtttgct gctgaggctg
    24481 gtattttttc caagtgaggt atttaatgcc gcgatcaatg aagcaccaaa ttcagcacgc
    24541 agattatcgc cggtagtcac accatatctg tttttatgat tcaccgtatc tgcttcaaat
    24601 gatgcgatag tatcatccat tacgaaggta aattttcctg ttgcgggcga catgaacaaa
    24661 ctaaatttct cacctttgcc gggcttccag tcgagacctg cggcagccgt aagatatccc
    24721 ggtgacatga tttttgaaat cggcaccaat gcatcaggat ccgctaaata gtcataaccg
    24781 ggtgcaaatt gagtaaggaa attcaccaat gctgaataat agaaatcatc agcaaatgca
    24841 tagcgaccgt atttgctatt taattctatg cggtcctcat tcttgcgaat atcagcatag
    24901 ttgtttgcag tgatgagtcc gtaattcatg tttagggtat tatctaaagt agtagtaccc
    24961 attttgtaat tggcatagaa atttgtcagg atagtacttg acaagttgct ttcaccccct
    25021 gctgcccaat tggtgagtga tgtttggttg aagtttagaa tagcgccacc accttttttc
    25081 caattggagg tagagtcggt ttgagcaaaa acagcagcat tgatggctat agctgctata
    25141 agcgtacaca tttttttcat gatagtaagt ttattataag catcccgcta tggtcaataa
    25201 tcaagccaaa aaaaatgccc tcgcgggtgg aaggcatttt tttgtgtgta tgtttatttt
    25261 ttgagaaggt cgcgaatctc ggtcaacaat accacgtctg caggtggtgc aggtggagct
    25321 gccggtgctt cctcttcttt tttcttcatg gaattcattg ctttcaccac cataaacatt
    25381 acaaatgcta cgatgaggaa ataaatgaca gcagatatga aatctccgta taatacagca
    25441 atttcaggct tgataactgt gccgtcagca gctttctcgg cggctttaag cctgagcttc
    25501 caggttgaga agtctttttg cacaatcaaa cttatgaatg gcattacgat tccctgaata
    25561 aatgctgatg atacagcagt aaaggcggca cccattacaa aaccgactga tatgtcgatg
    25621 agattgcctt tcatggcaaa tgccttgaat tcttttaaca tgctcatgtg ataaaatttt
    25681 agaggttaat ataaattaat gcacgattgg catgcaaggt ggaaaaactt tgcgaaatac
    25741 acaaatgaga catgtggaaa aaactatacg cgcgcctttg cgtgtaccgc attccgcacc
    25801 atttcccgat gttcaatgcc atcaagcaaa taaacatcgc ctacagcttc aaaaccgtag
    25861 tctatgtaga atttttctaa ataatattgt gcctcaatgc gaacaggcac tttgccatag
    25921 cggtcctcga taaaactgat ggccttatcc ataacctgtt tcccgtaacc ggcaccgcga
    25981 tgctcaggat gggtggctac tcgccctatg gaaacctccg ggtagctcgt tccgggttgc
    26041 acaattctgc aacaggcaat taccctgccg gcgtcatcaa caccaaacaa gtggtcgcaa
    26101 aattgatcct taccatccac atccagatag acacagttct gctcaacagc aaatacttcc
    26161 tgcctcaaac gcaaaatgcc atacaactcc gaaatattca gttgatgaaa actcttgatc
    26221 ttccaaaata attgttccat acccgtcgct taacctataa acgcaaacct gccaaaaaag
    26281 gttgtatccc gcttactcat ttttccacat cttgtgcggt aatttccggt tttgcacaaa
    26341 aaatgtgccg attttacatc aaaaaaagac ctgatccgtt ttgaagatca ggtctttgtg
    26401 taaaaaatat gggcagtgtc ctatccgcgc aaaatatttt tttcattgtt gaagaacctc
    26461 acacttacca tcaatgcaat acctgcataa acaaataatg caagatacgt caataacaat
    26521 gggcccattt caactgttcc cgaaataatt tctttagctc ccagcgacac atttaaaatc
    26581 ggtaccaaag cagtcccgta atctaatgtc accccgggta gcataccaaa tattgttgga
    26641 aacacaataa atatcatcaa tggtgtgatc atgctttgtg cctctttgaa gctgcgagca
    26701 tatatcgata acattaatgt tactgctgca aagaatatgt ttagcggtaa taaaatcaat
    26761 aacatcatgg caatagaacc gggttccata atgccattca gcaagtcagc aattt
//
LOCUS       DAOQHY010000020        26733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_796_length_26733_cov_6.237686, whole genome shotgun sequence.
ACCESSION   DAOQHY010000020 DAOQHY010000000
VERSION     DAOQHY010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 26733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26733
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_796_length_26733_cov_6.237686"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(250..894)
                     /locus_tag="PLL28_03020"
     CDS             complement(250..894)
                     /locus_tag="PLL28_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68316.1"
                     /translation="MIITEEDIPYSSAEKFRKFLIKNYISQILLAFAVVTMVIAAIAG
                     VVMILAVAFFAAIILALVSRLFLAITGRNLYSTVENETHGELQLPGNIQFKCIYEDKE
                     KLATIISTFQADASDFEDCDLVFISSDKAIQHIEERYFFRKYQTISDNIFLLEWHFDT
                     EHFSLGRIDLNASSYQTIKEVRRGVVPTLTVVDRSLIMRWNEPDFKKRITISGF"
     gene            complement(1020..1655)
                     /locus_tag="PLL28_03025"
     CDS             complement(1020..1655)
                     /locus_tag="PLL28_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68317.1"
                     /translation="MKKSLLLLAFTAYFLSARAQDTQYALKYDFFSPVAGCFGFSFEQ
                     PRNNFISFDYDLGLIGLRLDDYFTRDKFGGAYAAFGPRLYFDRDEAQTNDLRGAYFKP
                     QILVSYFQYQDNVDYYDYYSGLSYSGHLEGSDFSLSMLAAVGSQWIMSDLVVFDLWFG
                     LGYGGSWANETVDIPPGNYYDSGNNNFKYSHVHFGDSPLIFDGGLSIGFKW"
     gene            complement(1715..3013)
                     /locus_tag="PLL28_03030"
     CDS             complement(1715..3013)
                     /locus_tag="PLL28_03030"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF68318.1"
                     /translation="MLSIQKKLSNSFYAILAMPATAMGFALCVQISALSWILSTKYDL
                     NIEEIGIVWASGPLAGLVMQLLVGLVSDNAWFWGGRRRPFIIIGGTIAALMLLCLPNL
                     DAIQKLFGTNSLIPVALAVATTLDLAINLGFNPTRSIITDVTPDGKARTKGYTWMQTV
                     SGTLGVAAYFIGAWWGNYQLIYVGVFIILIFSIIPVFFIEEPRVLQADTTDAEINALD
                     IQAHTQKAEVNELVKIYLANAFSWIGVQTMFVFMFAFASQMLFNTADTGSLNDEQAKR
                     TGQIISYSFLILNAVGAILPAFVLEPLTKKFGRITIQSTCLFVMALGYGLIVFFGKNA
                     GILYTCMAICGIGWAAIVSLPFAIMSEKVNKQRMGLFMGIFNMSIVIPQLIVSLLIAQ
                     FVDDAEDKKMIFEISAITLFISALIWRSVREKRANASSVK"
     gene            3096..4487
                     /locus_tag="PLL28_03035"
     CDS             3096..4487
                     /locus_tag="PLL28_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF68319.1"
                     /translation="MLKVLNCERNSDKKMEKPRLSFWQIWNMSFGFLGIQFGFALQNS
                     NASAILQNFGADIHQLSWYWLVAPCTGMIVQPLIGHFSDHTWTRLGRRKPYFLVGTIL
                     CCIALVLLPNSAAFVAGKAALMIGAGFLMIMDASINVAMEPFRALVADNLPDKQRTLG
                     FSIQTFLIGVGAVVGSWLPYVLANWYDVSSSAPEGQVADNVIFSFYIGAGVFFTAIMV
                     TILFAKEYPPAEYERYHGKPQKGKNFLADIFTDFSRMPKTMRQLGLVQFFSWFALFGM
                     WVFTTPAIAHHIYHLPIEDDSSDTFREASTWVGVIFGVYNLVSAIYAIFLPMIARALG
                     RKLTHALSLTCGGIGLVSIFFAKSPEMLIFSMIGVGIAWASILAMPYAILAGSIPAGK
                     MGVYMGIFNFFITIPQIINGIFGGPIVHTIYNDNAIYAIVMSGIFMFCAAVSVLYVHE
                     EGDITAIRTKPIS"
     gene            complement(4519..5610)
                     /locus_tag="PLL28_03040"
     CDS             complement(4519..5610)
                     /locus_tag="PLL28_03040"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNF68320.1"
                     /translation="MSIDPNLPIRKRAVHFDLVRFIATFAVVQLHVATPWLWRESDTD
                     SYFWIVCIMFDTIGRAGVPIFLMISGALLLSHQEPIGNFFKKRFVRVFVPLIFWTVIY
                     EIRILADHHYLHGGVSADNIFEVLKNPFSGPVYDHLWFLYMIIGMYLVTPLLRKIVPV
                     LSRIDQWYLLGLWVFSDILLPLVYHYYDFSLGFDIPLVTPYLGFYLMGYYLMHHQFTA
                     KQIRIAWVVLISSYLLNVGMAWYDSHLHQGMQVFWLSHHRPGVFIMATSAFILLEHYA
                     MHAFSRFSEKKYKMLAYMGGTTFGIYLLHPLIIELFEGIFMGYIGIYPPFIHAVPFIA
                     IPVAAIIVTAVTMGIVTLMQRTPVLKKFV"
     gene            5805..6290
                     /locus_tag="PLL28_03045"
     CDS             5805..6290
                     /locus_tag="PLL28_03045"
                     /inference="COORDINATES: protein motif:HMM:NF024276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HNF68321.1"
                     /translation="MRAPYLLLAISVSAIMVLSAGCSKSESPESAALAFFQALYNEHD
                     LEAAEALVTDNSREQLHNDFKLIEGTVAMLSENEPMRYEYKTLDEQTTIKGDSAFVTV
                     WTSLDSSTFETLLIKEDGDWKVDFSFAGQHLNAEKLVDQAIQQAMETTPKVLVENNLN
                     N"
     gene            complement(6387..6893)
                     /locus_tag="PLL28_03050"
     CDS             complement(6387..6893)
                     /locus_tag="PLL28_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68322.1"
                     /translation="MSISKNKVVSLTYELREDNISGDLIEVVKADQPFVFLFGAGGLL
                     PEFESNLAGKMIGSSFDFQIMAANAYGEFDESAMEYVPKDIFMIDGKLAEDLLETDKV
                     INLRDQDGHLVRARVAEVGEAEVLLDFNHPLAGVNLHFSGQILDIRDATAEELDHGHV
                     HGPGGHHH"
     gene            7056..9017
                     /locus_tag="PLL28_03055"
     CDS             7056..9017
                     /locus_tag="PLL28_03055"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNF68323.1"
                     /translation="MKSFTLKSMILLLGCMTFFTANAQTAGKDEVLFTVAGQPVTKEE
                     FLYVYKKNNPDKQNDYSSESLREYLDLYVNFKLKVTEARAEKIDTTEKVREELQKYGD
                     QLIKSNFDKEVLEGAAQRMYDRMQSERLVYHIMIGIDPKANGADTAAAIAKLTAARER
                     LLKGEDFGKVAAEVSTDKSSPDNPGFVGWVVPGQINDLTFENTVFSTAPGNISNVIKS
                     RYGYHIVKVAKERSSMGTVTVEHILVRVPDNAKPEDFAKAKVRIDSIAAQIKAGNGSF
                     EEMAAKYSDDKMTAQNGGKLESFGTGKLANDFQEAAFALKNPGDISAPVKTSKGWHLI
                     KLIERKPMGTYAEMKDELKGKIERSPEYKELRADFVSKAKAKYGFTENADAKKEMYAI
                     MDSSFVKNSWTMNKAGNMNKPMFTIGDKSMSQADFASFLEINQRSSRDKSIEEKFSKL
                     YTQAVEQTVIEYDLSERNIDFRRLLQEYRDGLPLFAMLERKIWSKGSTDSVGLKNYYE
                     QHKTEYMWEERIDASIYTCKDTKTAKAVRKMASKHASTESILAKFNKDSLELVTVKSG
                     LYMTGQDSNIDKMNKQTGIGEDILNADGTITFVQINKIVPPTPKTLDEARGYVISAYQ
                     DQLEKEWIAELHKKYPVAINEKVFNSMVH"
     gene            9142..9882
                     /locus_tag="PLL28_03060"
     CDS             9142..9882
                     /locus_tag="PLL28_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68324.1"
                     /translation="MSDIAGLVSDNTSPEDSARIVDDYIDNWIRQNLILRVAEDNLQT
                     AMAKINQQADEYKESLVLYAYERQFLAENLDTVVAEDSLRSYYDLHKQDFTLQGDIYR
                     LAYAVVSKDDKTADSVSYWLNRGLEKSRLALERYCVNHADRFSLNTDIWLNENDLFTL
                     LPYDMYAGGRFRSKAPIAFSDTVNRYFAMVADFYVEGDTGPFAYFRNDIKDIIINKRK
                     KELLNDTYQRIYQEGLKRDNAELLKKEE"
     gene            9879..11213
                     /locus_tag="PLL28_03065"
     CDS             9879..11213
                     /locus_tag="PLL28_03065"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNF68325.1"
                     /translation="MKKIVIVIAMLLPIAAFAQPVVDKIVAQVGDKIVLMSEVESYYY
                     DALQQGEVPDDYRCAILQELITQKLMLIQAEKDSIVVTDDEVSSNIDNRIRYFIQMFG
                     SEEKMEEFYGKSVQEIKEEFREDVKNMLLIDRMRESIVGGMSVSPTEVKEFYNSIPKD
                     SLPFVNAQIEYSHIVMVPKANAEQKQIAKEKADGILKRIKNGEDFCKLASLYSGDGSK
                     DNCGNLGCATRDTYVTEFSAAAFRLQPGEISDVVETQFGYHIIRMDSRQGDKACLSHI
                     LIMPPVTNLNFVAANKKLDSLRAQIMAKNITFCDAAKKYSDDEQSSKSCGVVINPQTG
                     SGTFEISELSPDDYYSIENLKPGEVSQVLPYTTADGKKAVRIIMLNDQTEPHQLNLKD
                     DYAKLQTFTLNIKQNKEMESWIRTKAEREYIRIDNIYSGCSSLNELMLANGK"
     gene            11242..12207
                     /locus_tag="PLL28_03070"
     CDS             11242..12207
                     /locus_tag="PLL28_03070"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF68326.1"
                     /translation="MATFNNEVEAIDALAADYKKLQAEIGKVIIGQDQVVKDVLLSIF
                     SDGHSLLVGVPGLAKTLLIKTISDALHLSFKRIQFTPDLMPSDITGAEILDDQRNFKF
                     VRGPIFANIILADEINRTPPKTQAALLEAMQEKHVTIGGHMHPLDKPFFVLATQNPIE
                     QEGTYPLPEAQLDRFMFNIVLDYPAYEAELQIVRATTSDNKVTLNKVIGGEEIMAYQH
                     VIRRIPVTDNVLNYAVGLVAKTRPNTEKADAKANQYLSWGAGPRASQFLVLGAKCHAA
                     INGKYAPDIEDVRAVALPVLRHRIVRNYKAEADGISVEDLIKGMM"
     gene            12331..14229
                     /locus_tag="PLL28_03075"
     CDS             12331..14229
                     /locus_tag="PLL28_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNF68327.1"
                     /translation="MSDLIKHECGIALIRLLKPLSYYHEKYGTPLYGLNKLYLLMEKE
                     HNRGQDGAGIAAVKIDQKPGYKYFDRKRSISSSAIKDIFDEVFKKSHKMERKNPDLYH
                     NAEWAKQNVPFAGELLLGHLRYGTYGRNDIDSCHPFVRENNWITRTLLVAGNFNMVNN
                     DQLFQQLLELGQHPTQFVDTVTVMEKIGHFLDDEVQKLFDEKKEYHDNKTLSNIIGND
                     LDIQRILARAAKDFEGGYAMTGLFGHGDAFVLRDPNGIRPVYYYQDDEVVVAASERPA
                     IMTTFNVPFERVNELKPGHALIIKKKGSVKEAQCLQPRERKSCSFERIYFSRGNDKEI
                     YAERKELGRLLTDKVMKSVNYDFEHTIFSFIPNTAETAFYGMMKGLEQYLNNWKIAEI
                     KRLGNKITEEELHRILNMRPRVEKLAHKDVKMRTFITNDSDRSDLVSHVYDITYGQIN
                     DSSDSIVLIDDSIVRGTTLKQSIIAILDRLNPKKIIIVSSAPQIRYPDCYGIDMSKMG
                     DFVAFRAMTDLLKEQGKEQLIQDVYEECKTIQALPNNQTYNAVKKLYAQFSDEEISAR
                     IAKIVKADHIRAEVEVVYQSVEDLHKACPQNLGDWYFTGDYPTPGGNKVANKAFMNWV
                     EKKDVRAY"
     gene            14302..15546
                     /locus_tag="PLL28_03080"
     CDS             14302..15546
                     /locus_tag="PLL28_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNF68328.1"
                     /translation="MNRTFLLACLIMLIAPLSAQLSFTGAGVLLQNETVNNYPVIKQV
                     LKFSPASDAGLQAGEVVWSVNGQSTEGKTIKDVVAMIKGNNGESRTLVVGSDRRTVDI
                     TIRLIKGNCTDGDCNNGEGRIEEPNGDIYVGNFTNGKFDGYGMYYYYTGEHMYARYDG
                     HFVAGKREGDGVLDNYDGGYKFTGTFKGGQANGNATMTFYNNNNTYTGNFIADKPSGS
                     GTLTFADGSTKTFTPTTVDEILLEAGVKQETQIADNTDNKSNNNNTDNNNNASDSYTS
                     GYDFSEMLNKMANVEYNLQYAMEKYASFYSAYRTTFLDNNATAAEEASVDELKACVDY
                     VEKAFNLLQAINNSSAIGESVTELQYEAMKQWSKAVNEAMELFVAGLAQDATVSTWYW
                     MNISSDKMEDLLDEASAARHAF"
     gene            complement(15576..16286)
                     /locus_tag="PLL28_03085"
     CDS             complement(15576..16286)
                     /locus_tag="PLL28_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68329.1"
                     /translation="MKKMLSLIALSLIVNNILFSQDTLILKLNNPAPRVGQSVDLSMQ
                     VNMVLDAMRSTMDSTITFESTFQGGEKFLTQTLVFSDTGKHILGPFKFDINGKSYITN
                     TLEINVAPELPLTEGIWIRVVENNGNQILIVEEQIKKSEPKSRISFGSKKSDFDYYGN
                     QNSFEKFAYLNSEPEGAAIEFNRYWMQTYDSSNLSFFTGLYNIYFLEDSGVVYTLGAK
                     DFINLPDNITVPTVVIAQ"
     gene            complement(16345..16677)
                     /locus_tag="PLL28_03090"
     CDS             complement(16345..16677)
                     /locus_tag="PLL28_03090"
                     /inference="COORDINATES: protein motif:HMM:NF023123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HNF68330.1"
                     /translation="MKATITSLRLKSWVSFFPLSLYALRIMKQLKGSGHIEFKKTGFG
                     RMHYTMSLWKNEEDMRSFARSGAHLEAMRNSKKIAKEIRTITIDAESLPNWENAKKML
                     EQGKIIRF"
     gene            complement(16692..17069)
                     /locus_tag="PLL28_03095"
     CDS             complement(16692..17069)
                     /locus_tag="PLL28_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HNF68331.1"
                     /translation="MQTIATTLTILVAIEHLYILYMEMFAWETLGRKTFKGALPDHMF
                     TPTKTLAANQGLYNGFLAAGLIWSLIISDNIWSSNVAIFFLSCVVCAGIYGAATASKK
                     ILITQALPAALALAACLAARCMN"
     gene            complement(17382..19697)
                     /locus_tag="PLL28_03100"
     CDS             complement(17382..19697)
                     /locus_tag="PLL28_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68332.1"
                     /translation="MPTNFHIAPPPKTVDGLFAVPMDIQSINGSITFNAATSVALADI
                     TIDYIVGPTAGRPFFDLRQNISSAILDGAAFPVAQLASHNFDTGTYTTLRVIDDVQAA
                     LSYHTLQLQYTLGTPQSQLGGSYIPVLDWLAGPSLKFVFGLSDLNAARYLEAWIPANL
                     PFDQYSIDLDIIITNTLVPHTVITNATVTTVGTNHWQLSFPSRFSCVSPLLEIRATST
                     VESQTGNTVLPVSGKNITIEAFKLVGNAANLNTEINNIATFLAENEEAYGAYLHDNKF
                     TALFTTINGGMEYEGGTTTASWALNHETFHSWFARGMKPAAQSDGWWDEAFTTFHDDG
                     ANDTLPFDYSDAPITLCSRNPWQRTTPGNSYDDGGRFWQGIAAILGVNTTNNLMRDMH
                     NTYKGKSPVSTQMMEEFLICKSGNKDLVDAFHRFIYGFDDIGSPDLWMKDHTSHTGSD
                     QWDGAFWDSPDLWIRNSDDDGTTHQSPEYGQDNWFYARVRNKSATNEAQHFVVTFNHK
                     SWAGTQFLFPNDFLPAVAAKAEFTLGPGETRIVKAKWPRANIPAVGSHACVLASILCR
                     GDHPVNNRHVWEHNNLAQKNTTVVDLLPNAFIIIPVVLTNYFDLLSDYIIEAWRDRRM
                     EGYEVSLLHKDKMFFEGNDLKTIIHPDFSLKDHFKFDDRHALMDCGGHVDHYTQTIGM
                     ITDQRPDLLIRKLGNALEMPFSKEKVSGLSTNIPYQSQRVVGFKVKVPESAKPGTTFT
                     THLVQRHAKTKTITGGIAVTIHVADGRKTEK"
     gene            complement(19762..22707)
                     /locus_tag="PLL28_03105"
     CDS             complement(19762..22707)
                     /locus_tag="PLL28_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68333.1"
                     /translation="MNSKYESPLKAFVTRDKKRIARQVLHIQEPALSNQKIPRSAAFD
                     YIRNMADDFKISESHLQNMQKPLAYNTPIEQDMELRIVDEKRYFDSNTIRMAQTINNI
                     PIWRKGLTVTVKQNPNRIVYAANNTVASFRIKLTSEKAISRYQSMFASIAHKPIVPLL
                     SEEGQKDDPQTGDFVRGFAMNIKANAKTDRQAGKRNPARLIRGRMYYYAYDENKRQAV
                     HSHNITKDKVRVGEEHEHPSFPLPPIDKSIKNGQYYLVAEITFAWKIANEMQTWLALV
                     EMETGSILYIEPLFAGVSGKVFVQDPITSSGDTTQTANLNNATLNPYRTSVTLNNLDA
                     PVGGVQSLTGSNVQLVDVDGRTEPPPTKAVGDDFDYNVRTNDFAAVSAYYHTDNFFET
                     IESLGFDLSTYFTNTSFPVDIDHADQIDVNAHCMGNGVDGIDHSGYGYGDETNMVDPT
                     IGRAPDNWVHWHELGGHGILYDHVGTWYFNFAHSAGDGLAAIQNDPTSQLRELGNIER
                     FRYAPFRYPALDRWFNRDVAAGWGWDGSNDIGGYESEQILATSNFRWYRSIGGDSSSL
                     SRRQFASRMATYLILRAVSTLTPETNPTHAQGFCDALMAVDLLDWTSEGISGGAYNKV
                     IRWAFEKQGLFQPAGAPMPVTIAGAPPEIDVYINDGRNGEYEYQAVHWHNISMWNRQH
                     DDGGLTHEDAIPDATNYMYVKVKNRGTSNSGNVNVRGYHCLPGAGLTWPTDFTEMGPG
                     GGLNVASIAANNAEEVIVGPFEWVPNENIYGHDCTLMIVSTDGDPSNVDNFTLGDTVA
                     EWRLVPNDNNIGQRNVTILPGGGGENALIASLHDHFFWAGNPFRVQTKMQLKVELPKF
                     LAEAGWKVQFKDSKDQFTLKPGEKRKMVIDVIKGRDFSTEQVHAAYQRDIVIGLYAED
                     MLIGGMTYRIDPDKKEPGYIPGKKDRCTDKAQDLLDCLHLNSGKVKKVCIKKVSLDIE
                     LNSDNCQCD"
     gene            complement(23014..23517)
                     /locus_tag="PLL28_03110"
     CDS             complement(23014..23517)
                     /locus_tag="PLL28_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNF68334.1"
                     /translation="MSIIRLTTTIHCNIEVAFDLSRSIDLHIMSTKSTNEVAVAGVTS
                     GLISLNESVTWEATHLGIRQQLTAKITIFERPFHFRDEMVKGAFDYFKHDHFFEAQSH
                     TTIMTDVFEFTSPFGVVGKLFDRIFLTRYMTKFLVERNNTIKQIAESPDSYKFLHFDK
                     NGVHGKQ"
     gene            complement(23584..24105)
                     /locus_tag="PLL28_03115"
     CDS             complement(23584..24105)
                     /locus_tag="PLL28_03115"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNF68335.1"
                     /translation="MSFSIERSIEILERTPLVLKQLLSGLPEHWTGVNEGPETWSPYD
                     VIGHLIHGETTDWTKRMDIILSDGGNKEFATFDRFAQFQESKGKSLEQLLNTFHDERK
                     SNITYLLSKNISKTDLQKTGIHPKFGKVTLQQLLSTWVAHDLDHIMQISRVMAKQYRE
                     DVGPWKEYLKIMQ"
     gene            complement(24121..24510)
                     /locus_tag="PLL28_03120"
     CDS             complement(24121..24510)
                     /locus_tag="PLL28_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNF68336.1"
                     /translation="MHPDINIYHEALTESDKAIADTLASLIDKHLPNAESKIWHAHPV
                     WFIDGNPIVGYSKLKDSVRLLFWSGQSFEEPDLLPEGKFMAAEARFTNVNQIQKSDLK
                     RWLNKAAEIQWDYKNIVKRKGKLERLK"
     gene            complement(24678..25238)
                     /locus_tag="PLL28_03125"
     CDS             complement(24678..25238)
                     /locus_tag="PLL28_03125"
                     /inference="COORDINATES: protein motif:HMM:NF025703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HNF68337.1"
                     /translation="MKTNKIAIVAFVLLAALQIYLPASWAIESAETLSYGKVLKFKTI
                     PVDPKDLFRGNYIALNFENTRITVSDADDWKEGDNIYIVLNVDEGQFATYSHYEKDKP
                     RPDINFVTATVKHISRSPEPYVEIEYPFSKFYVEESIAKAAETAYRNAIAEGHNTYAV
                     VMAENGKTVLKDVYIDSKSLTDGLKD"
     gene            complement(25225..26508)
                     /locus_tag="PLL28_03130"
     CDS             complement(25225..26508)
                     /locus_tag="PLL28_03130"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HNF68338.1"
                     /translation="MLKDLHELVEAGILTEETAQQVSEYYDKKASTKGNRRLFVAFGI
                     LGAVLVGSGLILFIEHNWDLFSHLQKTIFAFIPLVLGQILCGTLLFRNRLESLWKEAA
                     SVFLFFSVGACIALISQIYHLKYSEEKILLIWMLLSIPIVYIMRSPMSSLLYIAGITW
                     YGGKYAFDYHEGFSRLYWLLIAMALPYYINLLQRKSINNFTSLHNWFIPISMSLCLTL
                     LTQDYDEALILMYFGYFSLIFAIGNSSLFSAVNTGKNGFKIIGFLGMLVILFISSNFI
                     AFDRGFYKSIHRFNFVKDDGFIFALALIITSGIVTFLNHRWAGKSKLALWEYTGFIFL
                     ATYVLNEFTVVAVILYNIYIFAAGITFIFLGSKRNHLGLMNLGLSIIAVLAIYKFFES
                     DLSYQLKGVLFMLVGAAFVTFNVIMLKRRRIYENQ"
BASE COUNT         8127 a         5421 c         5462 g         7723 t
ORIGIN      
        1 cgtgaatgca ccacatgctt cggtagacgc accccaggta agcgctacgt cgccactcac
       61 aagtgtttgc gcacagagaa gatcggcagg tatggtcgac tgtgcaaatg acattctacc
      121 gcccatcaaa gcaattgtta atatgcaaat acgcaaatac ttcactgtcg cagattggtc
      181 tttaactata acgcaaaatt gccataaata tttgtaatgc ctgctatctg tgcaacaagg
      241 gttctgtgct tagaagccgg atatggtgat gcgcttttta aaatcgggct cattccaacg
      301 cataatcagg ctgcgatcaa caactgtcag ggttggcact acacctcgtc tgacctcttt
      361 tatcgtttga taacttgagg cattcaagtc tattcgtccc agtgaaaagt gttcggtatc
      421 aaagtgccat tccagcagga aaatattatc acttatcgtt tgatactttc ggaaaaaata
      481 tcgctcttct atatgctgta tggccttatc ggaacttatg aaaacaaggt cgcaatcttc
      541 aaaatcagag gcatctgcct gaaatgttga gattattgtt gcaagctttt ccttgtcttc
      601 ataaatacat ttgaattgaa tgtttcccgg aagctgtaac tcgccgtgtg tttcgttttc
      661 aactgtagaa tatagattcc ttcctgtaat agctaaaaat aaacgcgaaa ccaaagctag
      721 tataattgcc gcaaaaaatg ccaccgccaa tatcatcacc actccggcaa tagctgctat
      781 taccatagtg acgactgcaa aggcaagtaa tatctgactg atataattct tgatgagaaa
      841 tttcctgaat ttctccgcac ttgaatacgg aatgtcctct tcggtgataa tcaaatttag
      901 acaggcttaa aaaataattt aggcaaaatt ataatgctca aatgataaat agattaaaat
      961 atcagtgtgc actaaatgta atcggcaaac acataaaatg ccgcccgatg aagtataaat
     1021 taccacttaa acccgataga taatccgccg tcaaagatca atggtgaatc tccaaaatgc
     1081 acgtgcgaat atttaaaatt gttatttccg ctatcgtagt aattacccgg tggaatgtca
     1141 accgtttcat ttgcccaact gccgccatat cccaatccaa accaaagatc gaatacaaca
     1201 agatcgctca tgatccactg tgaacctacg gccgcaagca tactcaatga aaagtcgctt
     1261 ccttccagat gacctgaata ggaaagaccg gaataataat cgtagtaatc tacattatct
     1321 tgatattgaa agtagcttac aagaatctgg ggtttgaaat aagcacctct aagatcattg
     1381 gtctgtgctt cgtcgcgatc aaagtataac cttggtccaa aggccgcata agcgccacca
     1441 aatttatcgc gggtaaaata atcgtcgagt cttagtccaa ttagcccaag atcatagtcg
     1501 aaggaaatga aattatttct cggttgctca aaggaaaatc caaaacaacc ggcaactgga
     1561 gaaaagaaat catatttcaa tgcatattgc gtgtcctgag ctcgggcgga aagaaaatat
     1621 gcggtgaatg cgagtagcag taggctcttt ttcattttag ggctttttgt aaagatagtt
     1681 aattcacttg atttgtaaaa ggggggatgt cacctcattt taccgaagat gcattcgccc
     1741 ttttttcgcg caccgaccgc cagatcagcg cactgatgaa caatgtaatg gcggatattt
     1801 caaatatcat ctttttatcc tcggcatcat caacaaattg agcaattaag aggcttacaa
     1861 tcaattgtgg aattacaata ctcatgttga aaatacccat aaacaaaccc atgcgttgtt
     1921 tattcacttt ttcactcatg atagcaaacg gcagacttac gatggcagcc caaccaattc
     1981 cgcatatggc catacaggtg taaagtatgc ctgcattttt accgaagaaa acaattaatc
     2041 catagccaag tgccatcaca aacaaacaag tagattgaat ggtaatgcga ccgaattttt
     2101 ttgtcagcgg ctctaataca aatgcaggta gtattgcgcc tacagcattt aaaatgagaa
     2161 aactataaga aatgatttgt ccggttcttt ttgcctgctc atcatttaat gatccggtat
     2221 ccgctgtgtt aaacaacatc tgcgaggcaa aagcaaacat aaatacaaac atggtttgca
     2281 cgcctatcca tgaaaatgca tttgccaaat atattttcac caattcattt acttccgctt
     2341 tttgtgtatg agcctgaatg tcgagcgcat taatttctgc atcggtggtg tcggcctgta
     2401 aaactcgagg ctcttcaata aaaaataccg gaatgatgct gaaaattaag atgataaaca
     2461 cacccacata aatcaattga taatttcccc accaggcacc aataaaatat gcggcaacac
     2521 caagcgttcc ggaaacggtc tgcatccagg tataaccttt agtgcgcgcc ttgccatcgg
     2581 gagttacatc agtgatgata cttcttgtcg gattgaagcc aagattaatg gcgagatcta
     2641 gtgtagttgc tacagccagt gccactggaa taaggctgtt tgtgccgaat aatttttgaa
     2701 tggcatcaag gttgggcaag cacaatagca tcaaagctgc aatggtgcct cctataataa
     2761 taaatggcct gcgtctgcct ccccaaaacc aggcgttatc gctgaccaat ccaaccagca
     2821 attgcatcac caaccccgcc aatggtccac tggcccatac aatgcctatt tcttcaatgt
     2881 taaggtcgta cttggtcgat aagatccaac tcaaagcact gatttgcaca caaagcgcaa
     2941 aacccatggc tgttgccggc atggccaata tggcgtaaaa tgaattgcta agttttttct
     3001 gaatggaaag catatttcgc tatttatgat tgctgaatat cacccaataa atataaaccc
     3061 cgaaatacct gtatggatac agacttggaa gtgacgtgct aaaagtatta aattgcgagc
     3121 gaaatagcga taaaaaaatg gaaaaaccac gattgagttt ttggcagata tggaatatga
     3181 gctttggatt cctggggatc caatttggct tcgccttgca aaacagcaac gcctctgcaa
     3241 ttctccaaaa ctttggtgcc gatatacatc aattgagctg gtactggtta gtggcaccat
     3301 gcacaggtat gatcgttcaa cctttaatag gtcatttcag cgatcacaca tggacaagac
     3361 ttgggcgaag aaaaccttac tttttagtag gcacaatatt gtgttgtatc gctttggtat
     3421 tacttcccaa ctcggcggcc ttcgttgcag gtaaagctgc actgatgata ggtgccggtt
     3481 ttctgatgat catggacgcc agtatcaatg tggccatgga accttttcgc gcccttgttg
     3541 ccgacaacct tcctgataaa caacgcacgc ttggttttag tattcaaaca ttcttgattg
     3601 gtgttggcgc agttgtagga agctggttgc catatgtgtt agcgaattgg tatgatgtct
     3661 cttcttctgc tcccgaaggt caggtagctg ataatgttat cttttcattt tatatcggtg
     3721 ccggcgtatt ttttactgcc atcatggtaa cgatattatt tgcaaaggaa tatccaccag
     3781 cagaatatga acgctatcac ggtaaacctc aaaaagggaa gaatttctta gcagacatat
     3841 tcactgactt ttcgcgtatg ccgaaaacga tgcgccagtt gggattggtg cagtttttta
     3901 gctggtttgc tttgttcggt atgtgggtgt ttacaacacc tgcaatagca catcatattt
     3961 accatctgcc aattgaagat gattcaagtg atacattccg cgaagcaagt acatgggtag
     4021 gtgtgatatt tggcgtgtat aatttagtaa gtgccattta tgccatattc cttcctatga
     4081 ttgccagggc attaggtaga aaactcacac acgcactatc gctcacatgc ggaggtatag
     4141 gtttggtttc catcttcttc gcaaaatcac ctgaaatgct cattttttct atgataggtg
     4201 taggtatcgc atgggcaagt attcttgcaa tgccatacgc catactcgca ggaagtatac
     4261 ccgcaggtaa aatgggcgtt tatatgggaa tatttaattt cttcatcacc ataccacaaa
     4321 ttatcaatgg catttttggc ggcccgattg tgcatactat ttataacgat aacgccattt
     4381 atgctatcgt aatgagtggt attttcatgt tctgcgcagc agtttccgta ctgtatgtac
     4441 atgaggaagg tgatattacc gccatccgca caaaaccaat cagctgataa attaatcgcc
     4501 ggttttttta cttgctgatc aaacaaattt tttcagcaca ggcgtccgct gcataagcgt
     4561 tacaataccc atagtaacgg ctgtaacgat gatcgcagct accggaatgg caatgaatgg
     4621 cacggcatga ataaatggcg gatagatgcc gatatatccc atgaatatgc cttcaaataa
     4681 ttcaataatc aacggatgta ataaataaat accgaaggta gtgccaccca tataggcaag
     4741 cattttgtat ttcttttcag agaagcgact aaatgcatgc atggcataat gctccaataa
     4801 aataaacgct gaagttgcca tgatgaaaac gccgggacgg tgatgcgata accagaatac
     4861 ctgcataccc tgatgcaaat gactatcgta ccaagccata ccaacattca gcagataact
     4921 ggaaatcaaa accacccatg caatgcgaat ttgcttggca gtaaattgat gatgcattaa
     4981 ataatacccc atcaaataaa atcccaaata tggcgttact aacggaatat caaaccccaa
     5041 tgagaaatca tagtaatgat atacaagtgg taaaagaata tcgctgaata cccataaccc
     5101 caataaatac cattggtcaa ttcgcgaaag caccggtact atcttgcgta acaatggcgt
     5161 taccagatac atgcctatga tcatatataa aaaccataag tgatcatata caggccctga
     5221 aaatggattc tttaatactt caaaaatatt atctgccgaa actccaccgt gtaaataatg
     5281 atgatcggca agaatgcgta tttcataaat gactgtccag aatatcagtg gtacaaaaac
     5341 tctgacaaat cgttttttga aaaagttgcc gatgggttcc tgatgactga gcaataaagc
     5401 accgctgatc attaaaaata tcggcacccc tgctcgtcca atggtatcga acatgatgca
     5461 tacaatccaa aaataactgt cggtatccga ctcgcgccat agccaaggtg tggccacgtg
     5521 caattgaacc accgcaaacg tggcaatgaa tcgcaccagg tcaaaatgca ccgcccgttt
     5581 ccggatcggc aggttcgggt caatactcat gcggcacaaa tttagtgttt tcaatcagag
     5641 gaaaactgtc gtcactaatt caatgaaatg tgcactttta agcaggtcat aaattcgatg
     5701 attctagatt gccgggctca gattgttaaa ttctggtaaa atattgaatt caatacccaa
     5761 tcctcacggc gaacagttta ttttgtatct caacccaagt cccaatgcgt gctccctatt
     5821 tgttgttggc catcagtgta tccgcaatca tggtattaag cgccggttgc agtaaatccg
     5881 aatctcccga atcggcagcc cttgcctttt ttcaagcact ttacaatgaa cacgatctgg
     5941 aagctgcaga ggctttggtt actgataata gtcgcgagca actgcacaac gactttaaac
     6001 tcattgaagg cacagtggcc atgttgtctg aaaatgaacc aatgcgttat gaatacaaaa
     6061 cactggatga acaaactaca ataaaaggcg actcagcctt tgtaaccgta tggacttcgc
     6121 tcgatagcag caccttcgaa acactgctga taaaagaaga tggcgattgg aaggtggatt
     6181 ttagctttgc cggtcagcat cttaacgcag aaaagttggt agaccaagcc attcaacaag
     6241 ctatggaaac tacccctaaa gttcttgttg aaaataatct aaataattga ttgtcaatta
     6301 tttgtgaaac atccctgagc acaattatag ggatgtccat tcaacacatc agtgtacagc
     6361 cgcctgtcca gttaaataag caccaattaa tgatgatgac cgcccggacc atgcacatga
     6421 ccatgatcca gctcttcggc tgtggcatca cggatgtcga gaatctgacc ggagaaatgc
     6481 aggtttaccc ccgcaagcgg atggttgaag tccaataaaa cctcagcttc accaacctcg
     6541 gcaacgcgag ctctaaccaa atgtccatcc tgatcgcgca gattgatcac tttatcggtt
     6601 tccagcaggt cttcagccag ttttccgtcg atcataaaaa tgtctttcgg cacatattcc
     6661 atggcagatt catcgaattc gccgtaggca ttggctgcca tgatctggaa atcgaatgat
     6721 gaacctatca ttttgcccgc aaggttgctt tcaaattcag gcagaaggcc gcctgcacca
     6781 aagaggaata cgaaaggctg atctgccttc actacctcta taaggtcgcc gctgatattg
     6841 tcttcacgca gttcatatgt cagtgaaaca accttgtttt ttgagatgga catgatttaa
     6901 aatttgttaa ggcgacaaag atacgctaaa aaaccagcga aacctgcatg atttttgctg
     6961 taaacggcga gccggagtgc tttgccaata cccgataatg attagctttg catcccccgg
     7021 ccaaaccgga ccaaacctaa atttagattc cacatatgaa aagtttcacg ttgaagtcga
     7081 tgatcctcct gctgggatgt atgactttct ttactgccaa cgctcaaaca gcaggcaaag
     7141 atgaagtatt gtttaccgtt gccggtcagc cggttaccaa ggaagaattt ctttacgttt
     7201 acaagaaaaa caaccctgat aagcagaacg actacagcag cgaaagcttg cgtgaatacc
     7261 tcgatctcta tgtaaacttc aaactgaagg tgaccgaggc aagggcagag aaaatcgata
     7321 caactgagaa agttcgcgaa gaactgcaga aatacggcga tcaactcatc aaatcgaact
     7381 tcgacaaaga agtgttggaa ggtgcagcac aacgcatgta tgatcgcatg caaagcgagc
     7441 gacttgttta tcatattatg ataggcatag accctaaagc aaatggcgca gatacagcgg
     7501 ctgctatcgc aaaattaaca gcagctcgtg agcgcctgtt gaaaggtgaa gattttggta
     7561 aagttgctgc cgaagtatca accgataaat caagtcctga taatcccggt tttgttggtt
     7621 gggtagtgcc cggtcagatc aacgacctta catttgaaaa tacggtgttc agtactgctc
     7681 ccggaaatat ttcaaacgtg attaaaagcc gctatggcta tcatatcgta aaggtggcta
     7741 aggaacgttc gtctatggga acagttaccg ttgagcatat tttggttcgc gttcctgaca
     7801 atgcaaaacc tgaagacttt gcaaaagcaa aagtgcgcat cgatagcatt gcagctcaaa
     7861 ttaaagcagg taacggaagt tttgaagaaa tggctgcgaa atactccgat gataaaatga
     7921 ccgctcaaaa tggtggtaaa ttggaatctt tcggtaccgg aaaattggca aacgattttc
     7981 aagaggctgc tttcgcattg aaaaatcccg gcgatatttc agcacctgtg aaaacatcaa
     8041 aaggatggca cctgattaaa ctcatagaga gaaaacctat gggcacctat gccgaaatga
     8101 aagacgaact caaaggaaaa attgagcgca gtccggaata caaagaattg cgtgccgatt
     8161 tcgtaagtaa ggcgaaagct aaatacggtt tcactgaaaa tgcggatgct aaaaaagaaa
     8221 tgtatgcaat aatggattct tcatttgtga aaaacagctg gacaatgaac aaagcaggta
     8281 acatgaataa acctatgttc accattggcg acaaatcaat gagtcaggct gattttgcat
     8341 ctttccttga aattaaccag cgttcttctc gcgataaaag cattgaagaa aaattcagta
     8401 aattatatac acaggcagtt gaacaaactg taattgaata tgacctcagc gaacgcaata
     8461 tcgacttccg tcgcttgttg caagaatatc gcgacggact tccgctgttt gctatgctgg
     8521 aacgcaaaat ttggagcaaa ggttctaccg attctgttgg cttgaaaaat tattacgagc
     8581 aacacaaaac agaatatatg tgggaagagc gtatcgatgc aagtatttac acttgtaaag
     8641 acacgaaaac tgcaaaagct gttcgtaaaa tggcttcaaa acatgcttcg actgaatcta
     8701 ttttagcgaa attcaataag gatagccttg aattagtgac tgtaaaaagt ggtttataca
     8761 tgaccggtca ggatagcaat atcgataaga tgaataaaca aaccggtatc ggtgaagata
     8821 ttctgaatgc cgatggtact atcacatttg tgcaaatcaa taagatcgtt cctcctacac
     8881 caaaaacact tgatgaagct cgtggttatg tgatctcagc ttatcaggac caattggaaa
     8941 aagaatggat tgccgaattg cataaaaaat acccggtagc catcaacgaa aaagtattta
     9001 attcaatggt acattgaccc tttgctgatc aggaatgtcg tcatagcatt aatcgtttgc
     9061 gtttcggttc agggttgtcg caaaggcaaa cccgagaaaa atgaggaacc gcttgcgaga
     9121 gtgtacgaca aattccttta tgtgtcagat atagccgggc tcgttagcga taacacctct
     9181 ccggaagata gtgcacggat tgttgatgat tatatcgata actggatacg ccagaatctg
     9241 atcttgcgcg tggcagaaga taatctgcaa accgcaatgg caaaaattaa ccagcaggca
     9301 gatgaatata aagagtcgct ggttctttat gcctatgaac gtcagttcct tgccgaaaat
     9361 ctggatacgg tagttgctga ggattcgctt cgcagctatt acgatctgca taagcaggat
     9421 ttcaccttac agggcgatat ctacagactc gcatacgctg ttgtatccaa agacgataaa
     9481 actgctgatt ccgtgagtta ttggcttaac cgcggattgg aaaagtcacg cctggcttta
     9541 gaacgctact gtgtgaatca tgccgataga tttagtctga ataccgacat ttggctcaac
     9601 gaaaatgatc tttttaccct gcttccatac gatatgtatg ccggtgggcg ttttcgttcc
     9661 aaggcgccaa ttgccttttc agacacggta aatcgttact ttgcaatggt tgccgatttt
     9721 tatgtggaag gagatacggg gccgttcgcg tattttcgca atgatattaa ggatatcatc
     9781 atcaataaac ggaaaaaaga gctgctgaat gatacctatc agcgtatcta tcaggagggt
     9841 ttaaaacgcg ataacgcgga attattaaag aaagaagaat gaaaaagatt gttatagtga
     9901 ttgccatgtt gctgccgata gcggcgtttg ctcagccggt agtagataaa atcgtagcgc
     9961 aggttggcga taaaattgtg ctgatgagcg aagtggaatc gtattactac gatgccctgc
    10021 aacaaggtga agtgccggat gattatcgct gtgccatttt acaagaatta atcactcaaa
    10081 aattaatgct tatccaagcg gaaaaagata gtatcgttgt tactgatgat gaagtaagca
    10141 gtaatatcga taaccgtatt cgctatttta ttcagatgtt cggatctgaa gaaaaaatgg
    10201 aagaatttta tgggaaatcc gttcaggaaa taaaagaaga atttcgagag gatgtaaaaa
    10261 atatgttgct gatcgaccgc atgcgcgaaa gtattgttgg cggtatgtcg gtttcgccta
    10321 ccgaagtaaa agaattttat aattccatac ctaaagattc attgcctttt gtaaatgcac
    10381 aaatagagta ttctcatatc gtaatggtgc cgaaagcaaa tgcagagcaa aaacaaattg
    10441 cgaaagaaaa agctgatggc atccttaagc gtatcaaaaa cggtgaggac ttttgcaaac
    10501 tggcatcact ctattccggt gatggctcaa aagataattg cggtaactta ggctgtgcta
    10561 ccagagatac ttatgtgacc gaattttctg ccgccgcatt ccgcctgcaa ccgggagaaa
    10621 tttctgacgt agtagaaaca caattcggtt accatatcat aaggatggat agtcgacaag
    10681 gggataaagc gtgcttgagt catatactca ttatgcctcc tgttactaac ctcaattttg
    10741 ttgccgccaa caaaaaactt gatagtttgc gcgctcagat catggcaaaa aatataacct
    10801 tctgcgatgc ggctaaaaaa tattccgacg atgagcaaag cagcaaatca tgtggtgttg
    10861 taataaaccc gcaaaccgga tccggaactt ttgaaatttc ggaattatcg cccgatgatt
    10921 attattcaat agaaaatctc aagccgggtg aggtttcgca agtactacct tatactaccg
    10981 ccgacggaaa aaaagctgtg cgtatcatca tgctcaacga tcagactgaa ccacatcagt
    11041 taaatctgaa agatgattat gccaagctgc agacattcac cttgaatatt aaacaaaata
    11101 aagaaatgga atcatggatc cgcacaaaag ctgaacgtga gtatattcgc attgacaata
    11161 tttacagtgg atgttcttca ttaaatgaat tgatgctggc aaacggaaaa taatttcaga
    11221 acagaggaag taatcgctaa catggcaaca ttcaacaacg aagtagaagc aattgacgca
    11281 ctggcagcag actataaaaa actgcaagca gagatcggaa aagtaatcat cggtcaggat
    11341 caggtagtaa aggatgtatt gctgagcata ttttccgacg gacactcgct attggttggt
    11401 gttcccggac ttgcaaaaac attgttgatt aaaacgattt ccgatgcact gcatttatca
    11461 ttcaaacgta ttcagttcac accggatttg atgccaagtg atatcacagg tgccgaaatt
    11521 ctcgacgatc aacgcaattt taaatttgtt cgcggaccaa tttttgccaa tatcattctt
    11581 gcggatgaaa taaacagaac gccgcctaaa acacaggcag ctttgcttga agccatgcag
    11641 gaaaagcatg taaccattgg cggacacatg catccgctcg acaaaccgtt ttttgtcctt
    11701 gctacccaaa accctatcga acaggagggt acctatccac tgcctgaagc tcaactcgac
    11761 cgattcatgt ttaacatagt gctcgattac cctgcttatg aagcagaact tcaaattgta
    11821 agagcaacaa cttccgataa taaggtaacc ctcaataaag taattggcgg cgaagagatc
    11881 atggcgtatc agcatgttat aaggcgcatt ccggttaccg ataatgtgtt gaactatgcc
    11941 gtgggattag tggcaaaaac acgtcccaat accgaaaaag cggacgcgaa agctaaccaa
    12001 tacctcagtt ggggtgcagg acctcgcgct tcgcagttcc ttgtccttgg agctaaatgc
    12061 catgccgcta taaatggtaa atatgctccc gatatcgaag atgtgcgtgc cgtagcattg
    12121 cctgttcttc gccatcgtat cgtgcgcaac tataaagccg aagccgacgg tatctctgtt
    12181 gaagatttga ttaaaggaat gatgtaaact tccgtaccgg ctgcatttgg cagggccgat
    12241 ggcgttttat gtacatgttg aggtattcca aagtctgtta ctcacctttt taatgattaa
    12301 ctttgcacct gtatccatta atacttacgt gtgagtgatc tgatcaagca tgaatgtggt
    12361 atagccctca ttcgactgct aaaaccgctg tcctattacc atgagaaata tggcactccc
    12421 ttgtacggtc tgaataaact gtacttactc atggaaaaag agcataaccg cggtcaggat
    12481 ggcgccggta ttgctgcggt gaagatcgat caaaagccgg gatacaaata tttcgaccgc
    12541 aaacgcagta tctcctcatc ggcgatcaaa gacatctttg acgaggtgtt taagaaatcg
    12601 cacaaaatgg agcgcaaaaa tcccgattta taccacaacg ccgaatgggc aaaacaaaat
    12661 gtccccttcg ccggagaatt gctgctcggt cacctgcgat atggcactta tggtcgcaac
    12721 gatatcgatt cctgccatcc cttcgtgaga gaaaataact ggataacaag aacattgctt
    12781 gttgccggaa actttaatat ggtcaacaac gaccaattat ttcagcaact gcttgaactc
    12841 ggtcagcatc caacacaatt tgttgatact gtaaccgtaa tggaaaagat agggcacttt
    12901 ttagatgatg aagtgcagaa attatttgac gagaaaaaag aatatcacga taataagacc
    12961 ctttcaaaca ttattggtaa cgaccttgat attcaacgta tcctggctcg cgcagctaaa
    13021 gattttgaag gaggttatgc tatgaccggt ttgtttggtc atggcgatgc atttgttttg
    13081 cgagatccga atggcatccg tccggtgtac tattatcagg atgatgaagt tgttgtggct
    13141 gcaagcgaac gcccggcaat tatgaccact tttaacgtgc catttgaaag agtgaatgaa
    13201 ttaaaacccg gccacgcgct gatcatcaag aaaaaaggtt cagttaaaga agcgcaatgc
    13261 cttcaacctc gtgaaagaaa aagctgcagt tttgaacgca tttatttctc tcgcggaaac
    13321 gataaagaaa tttacgccga acgcaaagag ttgggtcggt tgcttactga taaagtaatg
    13381 aaatcggtga actatgattt cgagcatacc atattttcct tcatccccaa tactgcagaa
    13441 acggcatttt atgggatgat gaagggcttg gagcaatatc tgaataattg gaagattgct
    13501 gaaatcaagc gactgggaaa taaaatcacg gaagaggaat tacatcgcat tttaaatatg
    13561 cgtcctcgtg tcgaaaaatt ggcgcataaa gatgtgaaga tgcgcacatt tatcaccaac
    13621 gattcagatc gaagtgattt ggtgagccat gtgtacgata tcacttatgg tcaaattaat
    13681 gattccagtg attctattgt tttaattgac gatagtattg ttcgtggtac tactctcaaa
    13741 caaagtatta tcgccattct cgacagactg aatccgaaaa aaatcatcat cgtttcttcc
    13801 gctccgcaaa tcagatatcc cgattgctac ggtattgaca tgagtaaaat gggcgatttc
    13861 gtggcattca gggcaatgac agatttattg aaggagcaag gaaaagagca gcttattcaa
    13921 gatgtttatg aggaatgtaa gactattcaa gcactgccaa ataatcagac ctataatgca
    13981 gtgaagaaat tatatgctca gtttagtgat gaagaaattt ctgcgcgcat tgctaaaatt
    14041 gtaaaagctg atcatattcg tgctgaagta gaagttgttt atcaatcggt ggaagatctg
    14101 cataaggcat gtcctcaaaa cctcggcgac tggtatttca ccggtgatta tcctactccc
    14161 ggcggcaata aagtagccaa caaggcattt atgaactggg tggagaagaa agatgtgcgt
    14221 gcatattgat ttttatcaat cgcaacaaaa cttctcaata caggcactaa atttgctatt
    14281 agcaacatag attttctact tatgaatcgc acatttttat tggcatgcct catcatgctc
    14341 attgctcctt tgtcggcaca actctctttc acaggtgccg gggttctgct tcaaaatgaa
    14401 accgtaaata attatcctgt cataaagcag gtattaaaat tctcaccggc atccgatgcc
    14461 ggattacagg ccggagaagt agtgtggtcg gttaatgggc aatctaccga aggcaaaacg
    14521 attaaagatg ttgttgccat gatcaaaggc aataatggtg aatcacgcac tttagtagta
    14581 ggaagcgaca ggcgaactgt agatattacg ataaggctga taaaaggtaa ttgcaccgat
    14641 ggcgattgta acaacggtga agggcgtatt gaagaaccaa atggggatat ttatgtagga
    14701 aatttcacta atggaaaatt cgatgggtat ggcatgtact attattatac cggtgaacat
    14761 atgtatgctc gatatgatgg acatttcgta gcaggaaaac gagaagggga tggggtgctg
    14821 gataattatg atggcggata taaattcacc gggacattta aaggcggaca agcaaacgga
    14881 aacgctacaa tgacatttta taataacaat aatacatata ccggaaattt tattgccgac
    14941 aagccatcag gttcaggaac actaaccttt gcagatggtt ctaccaaaac atttacacca
    15001 actacagtag atgaaatctt attggaggcc ggtgtgaagc aggaaactca aattgctgat
    15061 aataccgata ataaatccaa taataacaat accgacaata acaacaatgc atcagatagc
    15121 tatacttccg gctacgattt tagtgaaatg cttaataaaa tggcaaatgt tgaatacaat
    15181 ttgcagtatg ccatggagaa atacgcttca ttttatagtg cttataggac aaccttttta
    15241 gataacaatg caactgccgc tgaagaagcg tctgttgatg aactaaaagc ctgcgtcgat
    15301 tatgtcgaga aagcatttaa tctccttcag gcaatcaata atagttcggc aataggagaa
    15361 tcagttacag agttgcagta tgaggcaatg aaacaatggt caaaagctgt taacgaggca
    15421 atggaattat ttgttgccgg tttggctcag gatgcaaccg tttccacctg gtattggatg
    15481 aacataagca gtgataaaat ggaagacttg cttgatgaag catcggcggc aaggcatgct
    15541 ttctaatccg aaaaagcctt tttcactgat gatggttatt gtgcaatcac aacagtaggt
    15601 acagtgatat tatcaggtag attaataaaa tcttttgcac ctaaggtata cactacaccg
    15661 ctatcctcca gaaagtaaat attataaagg cctgtgaaaa aggatagatt actactatcg
    15721 taggtttgca tccaatatct attaaattcg atagctgcgc cttcaggttc tgaattcaga
    15781 taagcaaatt tttcaaaact attttggtta ccataataat caaaatccga tttttttgaa
    15841 ccaaacgata tcctggattt tggttcggat ttttttattt gttcttctac tatgagtatt
    15901 tgattgccat tgttttctac aacacgaatc catattcctt cagttaatgg caactccgga
    15961 gccacgttta tttcaagtgt attggtgata taagattttc cgttgatgtc aaatttgaaa
    16021 ggcccaagaa tatgtttgcc ggtatcgctg aaaacgaggg tttgggttaa gaatttttct
    16081 cctccctgga aagtactttc aaacgtgata gtgctatcca tagttgaacg catggcgtct
    16141 agtaccatat tcacttgcat cgacaggtca acagattgcc ctacacgtgg tgccggattg
    16201 ttgagcttta gaattaacgt gtcctgactg aacagtatgt tattgacgat caaggaaagc
    16261 gcaattaatg atagcatttt tttcataagg agtattttat agcattgatt tgattacaag
    16321 aaagtaatgt aattaaatta aactctaaaa tcgaatgatt ttaccttgtt caagcatttt
    16381 tttcgcgttc tcccaatttg ggagtgattc agcgtcaatg gttattgttc gtatttcttt
    16441 agcaattttt ttactgttgc gcatagcctc cagatgtgca ccactgcggg caaaggaacg
    16501 catgtcttct tcgtttttcc agagcgacat ggtataatgc attctgccga agccggtttt
    16561 cttaaattca atatgaccac tgcctttcaa ctgtttcata atacgcaagg catacaacga
    16621 caatggaaaa aaagaaaccc atgactttag gcgaagagaa gtgattgttg ctttcatagt
    16681 ttaataatcc gttaattcat gcatctggcg gcaagacaag cagctagcgc taatgctgcg
    16741 ggcaatgcct gggtgatgag aattttttta cttgccgttg ctgcaccata aattccggca
    16801 caaacaacac acgataaaaa gaaaattgca acattactgc tccaaatatt atcactgata
    16861 atgagcgacc atattaatcc tgcagccaga aaaccattat acagtccctg attggcagcc
    16921 agcgtttttg ttggtgtgaa catatgatcg ggtagggcac ctttgaatgt tttcctgccc
    16981 aaggtttccc atgcaaacat ttccatatat agtatataca gatgttctat ggctacgagt
    17041 atggtaagtg tggttgctat tgtttgcata tgcctgagcg cttgatatat tcaaatttcg
    17101 gaataattat ttgctattac aaatgcgatc atgcttaagc acgatgactg atttgcaggc
    17161 agagcgaagg agctattact ttgtaggcag tttatgacta aattgctgtg aataagaact
    17221 ccggaaacat tgatcatttc cgatatcaga atgatttttg ttcatttagt tattgagagc
    17281 agcatcccat ccgccctgct gaagtattgt tgcaaaatca taaagaaaaa cccattaaca
    17341 atctctgccc gcgggtatat ttactgatta gctcttgtaa actatttttc cgtctttctg
    17401 ccatcggcaa catggatggt tacagcaatt cctcctgtta ttgtctttgt ttttgcatgt
    17461 cgctgaacca aatgtgtggt gaatgttgtg ccgggttttg cactttccgg tactttcact
    17521 ttgaatccaa ctacgcgttg cgactgataa ggaatatttg ttgacagacc tgaaactttt
    17581 tctttgctga atggcatttc taaagcattt ccaagttttc tgataagcag atcaggcctt
    17641 tgatcggtaa tcattccaat cgtttgtgtg taatggtcaa catgtccacc gcaatccatc
    17701 aaagcatgac gatcatcaaa tttaaaatga tctttgaggc tgaaatcagg gtgtataatg
    17761 gtctttagat cattgccctc gaaaaacatt ttatccttgt gtaacagact tacttcgtaa
    17821 ccctccattc ttctatcgcg ccacgcttcg ataatatagt cggaaagtaa atcaaagtaa
    17881 tttgtcaata caacaggaat aatgataaat gcattaggaa gtaaatctac cactgttgta
    17941 tttttttgtg ccagattatt atgttcccaa acatgccggt tattaacagg atgatcacct
    18001 cggcaaagta ttgatgcaag tacgcaggca tgtgagccga cagcaggaat attggcgcgg
    18061 ggccatttcg cttttacaat gcgtgtttca cccggaccta atgtaaattc cgctttggcg
    18121 gcaacagccg gaaggaaatc atttgggaat aaaaattgtg tacctgccca tgatttgtga
    18181 ttaaacgtca cgacgaaatg ttgtgcttcg ttcgtagcac ttttgttccg aacgcgtgca
    18241 taaaaccaat tatcctgacc gtattcaggc gattggtgtg tagtgccatc atcatctgaa
    18301 ttgcgaatcc ataaatccgg agaatcccag aaagcaccat cccattgatc tgaacccgta
    18361 tgtgatgtgt gatctttcat ccataaatca ggagatccga tatcatcaaa cccataaatg
    18421 aatcggtgaa aggcatctac gaggtccttg tttccacttt tgcagataag aaattcttcc
    18481 atcatttgtg tggataccgg acttttacct ttataggtat tatgcatatc gcgcatcaga
    18541 ttattcgtgg tattgacacc aagaatcgct gctattcctt gccagaatct accgccatca
    18601 tcataagaat tacccggtgt tgtccgttgc caggggtttc ttgaacagag tgttattggt
    18661 gcatcggaat agtcgaaggg taatgtatcg tttgcgccat catcgtgaaa agttgtaaag
    18721 gcttcatccc accatccatc actttgtgct gcgggtttca tacctcgggc aaaccagctg
    18781 tgaaaagttt catgattcaa tgcccagctt gcagttgttg ttccaccttc atattccata
    18841 cctccattga tggtagtaaa taaggcagta aatttattgt cgtgcaaata tgcaccatat
    18901 gcttcttcgt tctcagccag aaatgtggca atattgttga tctcagtatt taagtttgct
    18961 gcattaccca ctaatttaaa tgcttctatc gtaatatttt ttcctgatac aggaagaaca
    19021 gtattacctg tttgagattc gacggtcgat gtggcgcgaa tttctaataa gggagataca
    19081 caactgaatc ttgaaggaaa tgataattgc cagtgatttg ttcctactgt agttactgtt
    19141 gcatttgtaa ttaccgtatg tggaaccaaa gtattcgtaa tgatgatatc taaatcaatg
    19201 ctgtattgat caaatggaag attggcaggt atccatgctt ccagatatct tgctgcgttt
    19261 aaatctgaca atccgaaaac aaatttaagc gaggggccgg caagccagtc aagaacggga
    19321 atgtaactgc cgccaagttg cgattgcgga gtgcctaaag tgtattgcaa ttgtagtgtg
    19381 tgataagaaa gtgccgcctg aacatcatca ataacacgta atgtagtata ggtgccggta
    19441 tcgaaattat gcgaggctaa ttgtgcaaca ggaaatgctg cgccatcaag aatggcagaa
    19501 gaaatgtttt gcctgagatc aaaaaatggt ctgcctgccg ttggtcctac gatataatcg
    19561 atagttatgt ctgctaatgc aacagaagtt gcggcattaa atgttatgct tccgttgatg
    19621 gattgaatat ccatcggaac ggcaaacaag ccatcaacag ttttaggtgg cggggcgatg
    19681 tgaaaatttg ttggcatgag catatgtttt taaaataaaa cggcagcact caaaattgaa
    19741 tgctgccatt ttcagatgaa tttaatcgca ttgacagttg tcgctattca attcgatgtc
    19801 gagcgaaact tttttaatgc agactttttt cacttttcca ctatttaaat ggaggcaatc
    19861 aagcagatcc tgtgctttat cagtgcagcg atcttttttt ccgggaatgt atccgggctc
    19921 ttttttgtcg ggatcgatgc gatatgtcat tccaccaatc aacatgtctt cagcatataa
    19981 tccgattaca atatcgcgct gataggcggc atgaacttgc tctgtggaga agtcgcgacc
    20041 tttaattaca tcaatcacca ttttgcgttt ctcgcccggc tttaatgtaa attgatcctt
    20101 cgagtctttg aattgcactt tccatcctgc ttcggcaagg aattttggaa gctcaacctt
    20161 caattgcatt tttgtttgaa ccctgaaagg atttcccgcc cagaagaaat gatcatgcaa
    20221 gcttgcgatg agcgcatttt ctccaccacc tccgggaagt atggttacgt tgcgttggcc
    20281 gatattatta tcgttaggaa ctaaacgcca ttcagcaaca gtatccccta acgtgaaatt
    20341 atctacgttg cttggatcac catcggtaga tacgatcatc agggtgcaat cgtgaccata
    20401 tatattttca ttaggcaccc attcaaatgg tccgacaatt acttcttctg cattatttgc
    20461 agcaatggag gctacgttta aacctcctcc gggtcccatt tcagtgaaat ctgttggcca
    20521 ggttaaaccc gcacccggta agcaatggta tccgcgaaca tttacattgc cggaattcga
    20581 ggtgccgcga tttttaactt tcacatacat gtaatttgtt gcatcgggaa tggcatcctc
    20641 atgtgtcagg ccgccatcat catgttggcg attccacatt gagatattat gccaatgcac
    20701 agcctggtat tcatattcac catttcttcc gtcattgata tatacgtcaa tttcaggagg
    20761 tgcaccggca attgttacag gcattggagc tccggccggt tggaataatc cttgtttttc
    20821 aaaagcccag cgaattactt tgttataagc accgcctgat atcccttctg atgtccagtc
    20881 aagtaaatca accgccatca acgcatcaca aaatccttga gcatgtgttg gattagtttc
    20941 aggtgtcaat gtacttactg cacgcaatat taaataggtt gccatgcgag aagcaaactg
    21001 tcttcggctt aaacttgaag agtcgccacc gatagaacga taccagcgga aatttgatgt
    21061 tgcaagaatc tgttcacttt cgtaaccacc aatatcattg ctgccatccc atccccagcc
    21121 ggcagccaca tcgcggttaa accaacggtc aagagcagga tacctgaatg gtgcgtaacg
    21181 aaatctttca atattgccta gttctcgtaa ttgagatgta gggtcatttt gaattgccgc
    21241 aaggccatca ccggcagaat gcgcaaaatt aaaataccag gtcccaacat gatcatataa
    21301 tattccatga ccacctaatt catgccaatg cacccaattg tcaggagcac gacctattgt
    21361 tggatcaacc atattggttt catctccata tccatatccg gaatgatcaa taccatctac
    21421 accatttccc atgcaatgag cattcacatc aatttgatca gcatgatcaa tatctaccgg
    21481 gaaagatgta tttgtgaaat aggtagacaa atcaaaacct aaactctcaa tggtttcgaa
    21541 aaaattatca gtatgataat atgcgcttac tgcagcaaaa tcatttgtgc gaacattgta
    21601 atcaaaatca tcacctactg cttttgttgg tggtggttca gttctgccgt ctacatcaac
    21661 tagttgcaca tttgatcccg ttaagctctg cacaccacca acaggtgcat ccagattatt
    21721 taaagtaacg gaagttcggt atggatttaa ggttgcatta ttgagatttg cagtttgtgt
    21781 tgtatcccct gaagaggtaa tggggtcttg cacaaatact ttgccgctaa ctcccgcaaa
    21841 caatggttcg atgtatagga tgcttccggt ttccatttca accaatgcaa gccatgtttg
    21901 catttcgttt gcaattttcc atgcgaaagt gatttcagca accagataat actgtccgtt
    21961 tttaatgctt ttatctatcg gaggcaaagg aaatgatgga tgttcatgtt cttcaccgac
    22021 acgcaccttg tctttggtta tattatggct atgcactgct tgacgtttat tttcatcata
    22081 agcgtaataa tacatgcgac cacgtataag acgagccgga tttctttttc ctgcctgcct
    22141 gtctgttttg gcattggctt tgatattcat tgcaaagcca cgcacgaaat ctcctgtttg
    22201 cgggtcgtcc ttttgccctt cttcagataa caatggaaca atgggcttgt gtgcaatgga
    22261 cgcaaacatt gattgatatc gagaaatcgc tttttctgag gtcaatttta tgcggaacga
    22321 ggcaacggta ttattcgctg catacacaat gcgattggga ttctgtttga ctgtaacggt
    22381 caatcccttt ctccagattg gaatgttgtt gatcgtttgc gccatgcgaa tggtgtttga
    22441 atcaaaatag cgcttctcgt caacgattcg caattccatg tcctgttcta ttggagtatt
    22501 atatgcaagc ggcttttgca tattttgtaa atgactttcg ctgatcttga aatcatcggc
    22561 catgtttcga atgtagtcaa atgcagctga tctcggaatt ttctgattgc ttaatgccgg
    22621 ttcctgaatg tgcaaaacct gtcgggcaat gcgttttttg tctctcgtta caaatgcttt
    22681 taacggcgac tcgtacttgg aattcataaa ttggttttta tggtgaaaaa atttatagcc
    22741 gattaaaatt cttaatcgac ttttcaaatg tattcaggct tttaccgtaa aaacaataga
    22801 gatagttcaa attcacggtt taccgttaat tcgaccaagt cacagatgct gtaagtttaa
    22861 atgcttgttt ggcaagcctt tcaaaagaat gaacatcgaa ttaaatgtcg tatttaattc
    22921 aaaaaatgtc acttttttga aaaatgtgta attttttcat taacggtttc cggttaataa
    22981 aaaattgaca attttatgtg tgaattattg tattcactgt tttccgtgaa caccattctt
    23041 atcaaaatga agaaacttat acgaatccgg ggactcagct atttgcttta tagtattatt
    23101 gcgctcaacg agaaatttcg tcatgtatcg tgttaaaaat atccggtcaa ataattttcc
    23161 caccacacca aaaggcgagg taaattcaaa cacatcagtc ataattgtag tatgcgactg
    23221 tgcttcgaag aaatgatcgt gtttgaaata gtcgaaggcg cctttcacca tttcatctct
    23281 gaaatgaaac ggtctctcaa aaatggtgat ctttgctgtt aattgttgcc tgattccaag
    23341 gtgtgttgct tcccatgtga cactttcgtt caaagaaatt aatcctgatg taacaccggc
    23401 tactgctacc tcattagttg acttggtaga cattatatgc agatcaatag agcgcgaaag
    23461 atcaaacgct acttctatat tgcaatgtat agttgttgtc aatcgaatta tggacatgaa
    23521 atcatttata cagccggctt taaagataga cttctaataa ttgaattgga aataatagta
    23581 cgctcactgc ataatcttca aatactcctt ccagggtcct acatcttcgc gatattgttt
    23641 tgccattacc cttgaaattt gcataatgtg atccaggtca tgcgctaccc aggttgacag
    23701 aagttgctgt aatgttacct tcccgaattt aggatgtatg cctgtttttt ggagatctgt
    23761 tttgctgata tttttcgaca ataaataggt aatatttgat tttctttcat catggaaagt
    23821 attcaaaagc tgttctaagg attttccttt actctcctga aattgtgcaa atctgtcgaa
    23881 ggttgcaaac tctttattac caccgtctga aagtataatg tccatccgtt ttgtccaatc
    23941 agtagtctct ccatgaatca gatggcctat aacatcatat ggactccatg tttcaggacc
    24001 ttcgttgacg ccggtccaat gctcaggtaa tcctgacaac aattgtttta aaactagcgg
    24061 cgttctctca agtatttcta tggagcgctc tatagaaaat gacataaaat gatgttttga
    24121 ttattttaat cgctccaatt taccttttcg tttcacaatg tttttatagt cccactgaat
    24181 ctcggctgct ttgttaagcc aacgttttaa atcgcttttc tgaatttgat ttacattggt
    24241 aaaacgtgct tcagctgcca taaactttcc ttcaggtagt aaatcgggtt cttcaaatga
    24301 ttgtccgctc caaaataata acctaacaga atctttaagt ttgctgtagc caactatggg
    24361 gtttccatca atgaaccata ccggatgcgc atgccaaatt ttgctttctg catttggaag
    24421 atgcttgtcg attaatgaag ccagtgtatc agcaattgcc ttgtcgcttt ctgttaaggc
    24481 ttcgtgatag atatttatgt caggatgcat ttgttaatta tcttgattta atcgactaaa
    24541 attaatctat attcttaagc cggcaagaag tttaactttt gacgaatagc tttaatatgc
    24601 ataatttgcg attttcaaaa tgcatttcaa gcagaataac ctaggaaccg gagtttagca
    24661 gataattgaa atgcgaatca atcctttaat ccatctgtca gggatttcga atcgatatat
    24721 acatctttca aaacggtttt cccattttca gccattacca cagcataggt attgtgacct
    24781 tcagcaattg catttctgta tgcagtttct gctgcctttg ctatggattc ttcaacataa
    24841 aattttgaga atgggtattc aatctcaaca taaggttcgg gagagcgact aatgtgttta
    24901 acagtagcag ttacaaagtt gatatccgga cggggtttgt ccttttcata gtgactatag
    24961 gtggcaaact gaccttcatc aacattcaat acaatatata tattatcacc ttccttccaa
    25021 tcatctgcat ctgaaactgt aatgcgggta ttttcgaaat tgagtgcgat ataatttcct
    25081 ctgaacaggt ctttagggtc aacgggaata gttttgaatt tcagcacttt accgtatgat
    25141 aatgtttcag cactctcaat ggcccagctt gcgggtagat atatctgtag cgctgctaat
    25201 aaaacgaaag ctacaatggc aattttattg gttttcataa atcctccttc ttttaagcat
    25261 gatgacatta aaagttacaa atgcagcgcc taccagcata aatagtacac cctttaattg
    25321 ataactaagg tcggattcga aaaatttgta aatagccagc acggcaataa tgctcagtcc
    25381 aagattcatc aagcctaaat gatttctttt tgagcctaaa aaaatgaacg ttattcccgc
    25441 tgcaaatatg tagatattat acaatatcac agccacaacg gtgaattcat ttaatacata
    25501 tgtagccagg aaaatgaagc cggtatattc ccatagggct agtttgcttt ttcctgccca
    25561 tcggtgatta aggaatgtaa caatgcctga tgtgataatt aaagcaagtg caaaaataaa
    25621 cccatcatcc ttaacgaagt taaatcgatg aattgacttg taaaacccac ggtcgaatgc
    25681 tatgaaattg cttgaaataa ataatattac cagcatacca agaaagccga taattttgaa
    25741 accatttttg ccggtattaa ctgctgaaaa aaggcttgag ttccctattg caaatattag
    25801 actgaaataa ccgaaataca taagaataag tgcttcatcg taatcttgcg ttaatagtgt
    25861 cagacatagt gacatggaaa tgggaataaa ccaattgtgt aatgacgtga agttgttgat
    25921 agatttgcgt tgcagtaaat ttatataata cggcagggcc attgcaatca acagccaata
    25981 cagccgtgag aaaccttcat gataatcaaa tgcgtattta cctccatacc atgtaatacc
    26041 tgcgatatac agaagtgacg acatgggaga acgcataata tacacgattg gaattgatag
    26101 caacatccag attaaaagga ttttttcctc agaatatttt aaatgataga tttgagaaat
    26161 cagcgcgatg caagctccta ctgaaaaaaa caggaaaaca gaagccgctt ccttccaaag
    26221 tgattcgagt ctatttctga atagtagtgt tccgcataat atttgaccaa gcaccaaagg
    26281 tataaacgca aatattgtct tttgcaaatg tgagaataag tcccaattgt gttcaataaa
    26341 taaaatgagt ccactgccta ccaaaactgc tcctaatatc ccaaaggcta cgaatagacg
    26401 ccgattgcct tttgtggatg cttttttatc atagtattcg gatacctgtt gggctgtttc
    26461 ttcggtaagg ataccggctt caaccaattc atgaagatct tttaacatgg ctgtgtgttt
    26521 tgatgcaaag taatccatgg attataattg ccggctatga aggaacgctg ttgtttgata
    26581 tttatgttac ggaaaatcgc aaattttccc gactttaccc aatcttgttg caaaaaatgc
    26641 aaatttaagg cttgagccct tctgagtcac ccggtaaagc tgaattttcc tcttttcgca
    26701 caaatctgtc ggcgacttct aaaatcgcaa tat
//
LOCUS       DAOQHY010000021        25957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_829_length_25957_cov_5.787661, whole genome shotgun sequence.
ACCESSION   DAOQHY010000021 DAOQHY010000000
VERSION     DAOQHY010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 25957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25957
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_829_length_25957_cov_5.787661"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PLL28_03135"
     CDS             <1..217
                     /locus_tag="PLL28_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68339.1"
                     /translation="FDYASEGWIWPYDPDAPTNRHLETPLDDKTFRTLIDFFLISPNV
                     ELLKIKTINNGFDFSDHQPVVMQVRLR"
     gene            complement(496..1557)
                     /locus_tag="PLL28_03140"
     CDS             complement(496..1557)
                     /locus_tag="PLL28_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNF68340.1"
                     /translation="MSDKPIISGIQQVGIGNPYVHEAWRWYRENFGMDVKIFEEAAEA
                     NLMLPYTGGMPHKRHAVLAINMNGGGGFEIWQYTSRKPVAPAFQVQYGDLGIYACKMK
                     SRDVQATYNFLKNRGINLLGDVTNDPAGNPHFYLKDLYGNVFEVVKGDDWFGKTKFST
                     GGSYGAVMGVNDIERSIKFYGDILGFDKIIYDKEDNFSDTAVLDGGNVRSRRVLLAQS
                     EDRRGPFSKLLGNGTIELVKVYDRIPNKIFKDRFWGDLGFIHLCFDIQGMDKMKTLCE
                     SIGHPFTVDSGNHFDMGEAAGHFSYIEDPDGALIEFVETHKVPILKKFGWYLNLKKRN
                     PEESLPTWMLKAMGLNRVK"
     gene            complement(1578..2375)
                     /locus_tag="PLL28_03145"
     CDS             complement(1578..2375)
                     /locus_tag="PLL28_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNF68341.1"
                     /translation="MFAGKVIWITGASSGIGEELAYQLATKKAYLILSARRTLELERV
                     REQCLVHTNCVDILSLDLSDNNALPEMAKRAEAIHGRIDVIIHSGGISQRDSMLNTVP
                     DAEARIMQINFTSVVTITRALLPGMIARGEGRIVLLSSVAGKIGVPNRTTYCASKHAI
                     IGYFDALRAELKIAKQPINIHIMVPGFIHTNISVNAVLGDGNPQGVMDPGQEKGMPVE
                     KCVQQMIRGMEKNKKEILIGGKEILMTYFRRFIPGLYYYLITRVKTK"
     gene            complement(2390..3094)
                     /locus_tag="PLL28_03150"
     CDS             complement(2390..3094)
                     /locus_tag="PLL28_03150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HNF68342.1"
                     /translation="MFLSIIIPTFNEARFIGNTLEFLLKQTYDMPFEIIVADGGSTDD
                     TTSIASNFPVSVLQSPERGRAQQMNFAARFAKGTILFFVHADCLPPANFSSFIRNALN
                     KGVDAGCFRYRFDSNKRMLQFNSFFNRFGGLLFRGGDQTLFIKKDIFSALGGFDESYI
                     VMEDYDMVRRISKGHRFEVLRDEAMVSARKYDENSWLKVNIANAVAMSMYLTGLFCPL
                     TIRNTYHRMIKHPKGR"
     gene            complement(3095..3619)
                     /locus_tag="PLL28_03155"
     CDS             complement(3095..3619)
                     /locus_tag="PLL28_03155"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF68343.1"
                     /translation="MPHQEPISIRLALSADAPLLAKLGAKTFFDTYQAFNTPENMTDY
                     ISKNFNINALEAELGDEAVSFFIAFSGDSGVGYIKLSQGVSSYVSENPAMEVSRYYVD
                     AGFQGRKVGAALMQSAFEHAKANNCKVIWLAVWQKNPKAVNIYIHQGFEIIGTTTFQL
                     GDDLQDDYVMVKRL"
     gene            3809..4183
                     /locus_tag="PLL28_03160"
     CDS             3809..4183
                     /locus_tag="PLL28_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68344.1"
                     /translation="MRKLLTILFALVLSGHFASAATADGEDLIRKATAVIDETVQHYA
                     AKEGEKKVSVSTILPDPDKQNVIITFTIDELNRIHVVEIQGGYAYLTHYIKSSLEGQT
                     IKSEHAIPGINYMMAIKLPASA"
     gene            4495..7038
                     /locus_tag="PLL28_03165"
     CDS             4495..7038
                     /locus_tag="PLL28_03165"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNF68345.1"
                     /translation="MFLMQAMNLHAQNPSWSASEIQLEIEKLTSSHSVLYIAAHPDDE
                     NTRLITWLANEKTVRTGYLSLTRGDGGQNLVGEEQGAYLGVIRTQELLAARRMDGGEQ
                     FFTRAVDFGYSKSASETFTKWPHDSLLADVVWVIRNFQPDVIITRFPTDERAGHGHHT
                     ASAMLAEEAFDAAADPTKFPDQLKYVSVWQAKRLFWNNSTWWDKQLPEKIAKADPTLA
                     VIDVGGYNAFLGKSYGEIAAESRTNHKSQGFGSAPTRGEQKEYLELKKGEAFSNNDIF
                     SGLPIDWSVYENKVSALALASSAFQNFDAKHPDRSIPILIDYIKTLETFPQDPIVIRR
                     IQQAQQIIGACIGLFLEPAATTEKVAAGESLTIVSTAVKRLDYPITLESIEILGKTYK
                     EGEILPTNINQIDTIIFEVPEGITSSPYWLENDYNTLFDVRDRSLIGKAENDPAISVI
                     YHVKIGDRSFAFKRGVIYKETDAVKGEIIQPLTVLPKALIRANKSLLMFRYNEPLFVD
                     VIVESNIKDLDGFVFVSNDEGFIVSGGREIRTLQAGQTIAVQFSIQAPTNSKVETGNL
                     KLYFIPGKNAKAIYDSLIHSGVYHAVENYIVGSKKGVYRDLQTISYDHIPIQQILEHA
                     EVRMVRIQSPVPTFPVAYIEGAGDKVDESLRAFGVQVTTINPEDITQALLQQFPVVII
                     GVRAYNTSKAMAEKQALLMDYVQAGGTLITQYSTNWDSYVDQLGPYPFKLTRNRVTDE
                     QSPVSFLLPDNPALNTPNKITENDFKGWVQERGIYFVGEADSSYAFPLGLTDPGEKEQ
                     NGALMVAHYGNGVFIYTGLVFFRELPAGVPGAYRLFVNLMSMRGKSQPK"
     gene            7142..8875
                     /locus_tag="PLL28_03170"
     CDS             7142..8875
                     /locus_tag="PLL28_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNF68346.1"
                     /translation="MSTIDWLVMFGTLLLIAVYGIYRTRQIHTAETYLRGDNEKRWWE
                     IGLSVMATQASAITFLSVPGQAYDNGLGFIQFYLGLPLAMIVISVFFIPIFYRLKVYT
                     AYEFLENRFDVKSRTLTAVLFLVQRAFSTGITIYAPSIILSQLLGWPLTSTVIFIGGI
                     VTLYILSGGNKAVTVTHKQQMLVIYAGMFTAFGFLAYYITRDINLGEGISLAGYMGKM
                     HVITTGSESGHFDWSDRYNIVSGLIGGLFLQLSYFGTDQSQVGRYIGGASVRESRLGL
                     LFNGLVKIPVQFFILFIGLLVFTFYQLNTPPVYFNDVAYQKALHSDYGPQLEKLASDY
                     QLASELQQSNARQTVNRKEQTIYEYQESGRKADSIRLEVQKIVEKVEPSTQDDHDYIF
                     MRFVMDHLPVGVIGLLFAVIFCASMSSTASALNGLSTTFSVDIYKRFMFKNATGKQHL
                     QASKMLTAAFGILAIMFALIVSMFDNLIEAVNILGSLFYGTILGIFLTAFLLKRVGGN
                     AVFVAAVISELVILYIDFSTRYHWNTPTIQIGYLWYNVIGCLLVMGISLVLSFILPKY
                     EIISEGIDLPE"
     gene            8992..9786
                     /locus_tag="PLL28_03175"
     CDS             8992..9786
                     /locus_tag="PLL28_03175"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNF68347.1"
                     /translation="MRQYHALLQRILDEGTRKSDRTGTGTVSIFGHQMRFDLQEGFPL
                     VTTKKLHLRSIIHELLWFLKGDGNIAYLKENGVSIWDEWADEHGNLGPVYGVQWRSWP
                     TADGRHIDQISNLINQIKKSPDSRRLIVSAWNVADIENMALPPCHCMFQFYVADGKLS
                     CQLYQRSADTFLGVPFNIASYALLTMMVAQVCDLQYGEFIHTFGDAHLYNDHIEQAKL
                     QLSREPRVLPQMKINPDVKDIFGFKFEDFELVNYDPHPHIKAKVSV"
     gene            9796..10293
                     /locus_tag="PLL28_03180"
     CDS             9796..10293
                     /locus_tag="PLL28_03180"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HNF68348.1"
                     /translation="MPLFSIIVAASENEVIGMQGDMPWLKLTSDLKYFKEKTSGHWCI
                     LGRKTYNALGNKVLPNRKFIIVTRDRNFKSDDSIVVHDLNEARHHPLIQQEEEVFILG
                     GGDIYRQAMSFVDRIYLTRIHASFEGDTYFATPGEGFHLASKDVRTIDEKNPYDHDFL
                     VYEKN"
     gene            10311..11558
                     /locus_tag="PLL28_03185"
     CDS             10311..11558
                     /locus_tag="PLL28_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF68349.1"
                     /translation="MNNNQRRTTIFNLTVLVAALGYFVDIYDLQLFNLVSKTSLKGIG
                     ITDLSLVDYWDVRLFNVQMIGMLVGGIIWGIMGDLKGRKSILFGSILLYSAANILNGF
                     VDNTTQYQVVRFFAGLGLAGELGAAITLVAEIMSKEHRGYGTMLIVSVGALGAVAAFF
                     ITSHVDWRVSYYIGGGLGLALLLLRFGTFESTMFDEVKQQNVARGQFLSLFSDRKRFL
                     KYVYCILIGIPVWYCIGVLMKFSERFAKEWGIEMTPEEAIQVRRTAIMLSYIGLCVGD
                     LLSGYLSQVFKSRKKIVMGYLIATVVLALVYLNMKSSSLTLFHIMCFLLGCATGYWAL
                     FVTIASEQFGTNIRATVTTTVPNFVRGLVVPLTLSFAGLSVSVGNIQSAMVVGAVSIG
                     IAMIATLSIKETFGKDLNYMELS"
     gene            11686..13092
                     /locus_tag="PLL28_03190"
     CDS             11686..13092
                     /locus_tag="PLL28_03190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68350.1"
                     /translation="MRTLKHLFLTGILLPLSFAYAKACDYSHFDLVSTTDLGGGQYEF
                     VVTFCAGGGEGGADQSTYTWGVELVGPATFSSWPATLASPQTGAIYAADGSFYYGSNY
                     LIYFLDSYTGTGFAAQSWTTASAGYGPAGSYCKTFTIVTNGMPTQLRLMGAEAAGIAV
                     EPYGCNGAPDMEINFDVAADAGLTTSLCAGNTVTLNATATGGTPPYSFVWNTGATTAS
                     INVSPTVNTTYTVTVTDANGHTDSDPVVVVVHPKPTVNAGTDKTITIGYGATCATLTG
                     TATGGSSPYTYSWTGGGTSLTKTVCPTTTTTYTLTATDYYGCSNSDAALVTVKDVRCG
                     PSLTKVYVCKMGTTKCITTGQVPSYLTSGWVLGACWMKLDDEVALSENPIDIFPNPAT
                     TSAEIVFVMTEDGEATIELYSLNGERFPIADAVVAGRAGEEISHVIALDNIPAGIYQI
                     FVHTTSGQIMTEKLAVIR"
     gene            complement(13156..13590)
                     /locus_tag="PLL28_03195"
     CDS             complement(13156..13590)
                     /locus_tag="PLL28_03195"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNF68351.1"
                     /translation="MYYTYQITINKPVDEVVRLFDNPDNLYKWMSGLQSFELISGTAG
                     EPGAKSKLVFLNGKRRMEMIETITVRDLPAEFSGTYEAQGVVNISVNKFKDLGNGTTL
                     YTTENTFHFSGFMKLIGFMMPGAFKKQSYKYMEQFKAFVESH"
     gene            complement(13672..14412)
                     /locus_tag="PLL28_03200"
     CDS             complement(13672..14412)
                     /locus_tag="PLL28_03200"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNF68352.1"
                     /translation="MKVLIIDNETPIRVALHEMLLAYCPEITHIKEASDIKTGIDTIG
                     WFNPDILFLDVELDEGTGFDLLSQLPAYTFQLIFITAHDKYAINAFRFSAMDFLLKPI
                     DPDDLMHSVGRAIKNIQSDPSKQMAFLLEQVKSKHEPPKKIILKDMHKTYYVRIEEIL
                     FLEAEGIYTKFNLMGNQQIMVSKNLKEYESMLEPLGFVRTHHSFLVNPEKIKMFDKSE
                     GGMLVLEDGNQIPVSQRKRDLVLKALEH"
     gene            complement(14393..16291)
                     /locus_tag="PLL28_03205"
     CDS             complement(14393..16291)
                     /locus_tag="PLL28_03205"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNF68353.1"
                     /translation="MFNQFQLKGLLLLLLVCSSLLTKAQHDCSCASYNKSFRDQYEAL
                     FSMRKLDSARLLSNTLIKQKGCCEAAGNILVANTFLIDKQFDSIGKFVDRAEKLIGNN
                     YDTLVSPEILRLRGAVAAGKADLDKAASYYAEGIEQSKKHQNLKYAARFCNELSHTYG
                     SMNKPEPSLKFLKEGAAIAEQIKDDKLCAMLYANILATYGMFHENTHNKAYLDSMEMY
                     APISIKYAQLSKDPYYIVRGYTSLGGIAIEKGDYKTALAYNDTVMAMLPPAGAEQLQI
                     SAQYRRGQCLLQLGRVEEALASLHAALGNAKKFKSQGIVSLINQQLYFTYRDLGKSDS
                     ALYYYEQHVAVRDSLVNIENTSALTELEQKYDKVQNEKKISELSKEKQISTLQIRILA
                     AGILVTLLVSLSIFLYYRQKTLNNKKLILETEQRLNRARMNPHFFFNALASLQGFALR
                     ENDGKALASNLSKFSHIMRETLESTYKDYVTIQQEIDFLYEYLTLQQIRFPQAFKFVM
                     SADALSEPDSLLIPSMIIQPFVENSIEHGFRGIDYVGEITILFDEDGDNIVVSILDNG
                     KGLAGEKEITNEHISRASQIIRDRIYLLNLKLKTNASFQIANQRDGKGVQVVIRLPKI
                     TEDESLNN"
     gene            complement(16719..17471)
                     /locus_tag="PLL28_03210"
     CDS             complement(16719..17471)
                     /locus_tag="PLL28_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNF68354.1"
                     /translation="MLKAIIIDDESRARSALKQEITLNCPDVHIIGEADSISASVSLL
                     EKADPDIIFLDIQLSDGLGFTVLEQIENDDYAIIFTTAYSEYALKAFKTSAVDYLLKP
                     IDSEELVAAVNKVKNAKEEKHLQQVANSARRDLNALQQKKIILHTTEGLHVIPVGDII
                     RCNSYGNYSFVFLKDGSKILLAKILKEIEESLQHYGFERVHHSHLINLNHVKSFRNKD
                     GGMVMMSDMSEVPVSMRKKSAFLDELEKLNSL"
     gene            complement(17483..20419)
                     /locus_tag="PLL28_03215"
     CDS             complement(17483..20419)
                     /locus_tag="PLL28_03215"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNF68355.1"
                     /translation="MKAGFRQLKRTKQLVVKELLYALLFVVLTQWHLSLVGQHPYIVP
                     VTPADDYLPTNSFYVFRDNSDVMWIATNSGVYRYDGYAFTHFTSADGLGDNEILRIYQ
                     DKKNRIWFQPINGNPSFYLNGSIHNAQNDSLVKQLEFNKMVLAECEDDRGNLYLAGRG
                     PVVYKINDRDEVSQISVSGLENFIWVAKDGSLKMMDKASFPTRIIRADTYGSMHLISN
                     EQDVYSLKDDNILEKEFTLPQGSAEIIFIKFINYEEVFIGTRNGLFIWNRITKDYKYL
                     MPGYSVSSAERDFEGNTWISTLQSGVYLMPNVNVSFWNSDYGLSEDKITALEADADGR
                     LWIGCANNHYAIMENGKIIADRRLPEPTPMDIMNIRHFGDRTYVVGKAGILGMGKGFN
                     KSYGVYANDLIFYNDMVMIGQDNAMLFTRDEFETRIKQLVFNYPERKRDYEVINARTN
                     VFCQINNAIWIGTSRGLYIYENGKTSFVGDHIAACKNPIRDILFDSRTGLQFVGTMNG
                     LLVLRDEQLVTIIDKNSGLPNSDCNAIFLDKEGIIWAAFGNQLVAITKNGENFTTINY
                     TKALRLDPSRITDVHVAQGKVFISTESGLIAFDQNIELPNPVPPRIIISSFQVNGKES
                     ELGKDVRLHWKQNDITVQYSGMSFTSKNRITYTYYLSGYDTKWHNTTDRSIQLKSLPP
                     GDYKFLVKATNIAGISSPVSTISFSINKPVWGEVWFIILSVCALAGIILIGWRMRLRA
                     VKQSFTVKQRTIQLEMEHAEAERKIEQLNQQVFRQQMNPHFIFNALNTIKGYYAENDI
                     KKASDYISKFSKLLRNILENNEQWIPIEREINAITLYLDLAAMRYAYKFTYNISAHQP
                     LQVMRTGIPPMLLQPFIENALVHGIAPKQGSGHIAIDFSVSDNKLICIVEDDGIGRKA
                     SATRSRISEHNSKATQIINDYLQALNQRERAEAFILDIDDLTDETGKGIGTRIRLSMP
                     LIILNP"
     gene            complement(20677..22152)
                     /gene="proS"
                     /locus_tag="PLL28_03220"
     CDS             complement(20677..22152)
                     /gene="proS"
                     /locus_tag="PLL28_03220"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNF68356.1"
                     /translation="MAEKITKRGVNYSEWYNDLVIQSGLAEYAAVRGCMVIKPYGWAI
                     WENMKTVLDTKFKETGHVNASFPLLIPKGFLEQEEGHAEGFAKECAVVTHYRLKTNPD
                     KKGKLMVDPESRLEEELIIRPTSETIIWNCYKGWIQSYRDLPILINQWANVMRWEMRT
                     RLFLRTAEFHWQEGHTAHATSEEAVEETVKMLNVYADFMENYMAVPVIKGVKTANERF
                     AGAVDTYCVEGLMQDGKALQCGTSHFLGQNFAKAFDVKFLTREGKQEYAWATSWGVST
                     RMMGALIMAHSDDEGLVIPPRLAPLHVVIVPIYKTEEQLAAITAIAEGIMKELKDLKI
                     TVKYDDNDQAKPGWKFAEYELKGVPVRIAMGGRDVENGTVEIARRDTKEKVTVKNENL
                     GLYIQQLLEDIQQNLYNRALQFRTASTHEVDSWDDFKKQIEIGGFVSAHWDGTSETEE
                     KIKEETKATIRCIPLDAVQETGNCVYSGKPSTQRVLFARAY"
     gene            22244..23836
                     /locus_tag="PLL28_03225"
     CDS             22244..23836
                     /locus_tag="PLL28_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68357.1"
                     /translation="MKLNYLIMLAATGLMAASCATQTASRGASSDDIYYSAGDPTYTE
                     ESIKTETTTSSSNYSTYPGTSAQTEYAYSEPQDDAGYRMGGDDLSSSNEYYDDEGNTY
                     IVNNYYDDYDYDDYYYTSSLYRFYSPYYGFGYHSHCYSPYYYDPFWGPSYGWNVGYSW
                     GWGGIYYGSYYPYYPYNYGYCYNPYGYYGYYDPWYNPYYGYGGYYNGYNNGYYNGYWN
                     GYYDGYYDNGYYSGNYGGYYGNSDDYYYGVNTGTGSNTNTGDPSANILSAGYDKAGNG
                     TTDMNHILTDKGSLVVDKGDVNMTAAAANDNNVAGVAELPSIKDKTLSDHSVVAAINE
                     PVALNSSSTGVTEGTTRTDAGVRVIKYYSADAQNASVQTDRPQPVVSDRGPRGDYGTS
                     VNKPTTTGNNTVTNGVKNNTTSRPEYQQTPSREPVYNNNQTQRPDQPSYDRPSQTKPS
                     YEQPKQPKQPTYEQPRNNTPSRSNDAPSKPSKSYDTPKSNSGSSSSRPSYNSGSSSRP
                     SNSGSSGSKPGGSSPNKRSGGN"
     gene            23872..25377
                     /locus_tag="PLL28_03230"
     CDS             23872..25377
                     /locus_tag="PLL28_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68358.1"
                     /translation="MRKLIIMVIATALSVSSFAQSDVDALRYSMLDFGGTARSLGSGN
                     AYGALGGDMSVTSMNPAGLAIYRSSELTITPGLLSINSDATYYGQSTTDDRYHFQLNN
                     VGLVFANVNDGKANATDGWVGGAFALTYNKVANYNNAVTYSGFNDGSSLLNTYADFLN
                     SGGGTNPGDVFDKDPFGAGLAWETYLLNPLGTDSTQYYPLITDGNVQQTKVMQTDGGM
                     GEFDISFAGNYGNRLYIGATIGIPHIRYVQSYTWKEEDINNEYNDFNSYSLVDVTETY
                     GTGINAKFGLIYRLNDYLRLGGAVHTPTLFGMSDNYYSSMSSDLNSGDYSYDSPIGEY
                     NYELVTPWRVIGSAAVTLKNIGFISAEYEFVDYSESFFNFNRTLDAGDLSYETTVNTN
                     IDTKYGASSNIRLGAELMYDVFRFRAGYIMSATPFNDGIAAEGADLAKNTYTAGIGIK
                     DKSYFIDLAFAHSNIKQYDIQYVYDNGSGVNDGAVINTSINNFLLSFGFRF"
BASE COUNT         7379 a         5378 c         5193 g         8007 t
ORIGIN      
        1 attcgattat gcctcggaag gttggatctg gccttatgat ccggatgctc caacaaaccg
       61 acatctggaa acacctttgg atgataaaac tttcagaaca ctgatagact tctttttaat
      121 ttcaccgaat gttgaactgc ttaaaattaa aaccatcaac aatggatttg atttctccga
      181 tcatcaaccg gttgtgatgc aggtgaggtt gaggtgagtg ggtgagtggg tcagtgggtg
      241 agtgagcttg cctcgccatt ggcgggtggg tcagtgggtg aatgagaata aaaaggatta
      301 atcaataaag aataagtaac ttatccttca aatcttcaaa ccttcaaatc ttcaaacctt
      361 cgaatcttca aaaccttcaa atcttcaaac cttcgaatct tcgaaccttc aaaccttcaa
      421 accttcgaat cttcaaacct tcgaatcttc aaaccttcga atcttcaaat cctctacata
      481 cccccttttt cctaatcact taaccctgtt caaccccatc gccttcaaca tccaggttgg
      541 aagtgattct tcagggttgc gctttttgag gttgagatac cagccgaatt ttttgaggat
      601 aggcacctta tgtgtttcta caaattctat cagtgctcca tcaggatctt cgatgtaaga
      661 aaaatgtcct gcagcttcac ccatatcaaa atgattcccc gaatcaacag taaatggatg
      721 tcctattgat tcgcaaagcg ttttcatttt atccatgccc tgaatatcaa agcacaggtg
      781 aataaatccc agatcacccc agaatctgtc tttgaaaatt ttattcggaa tgcgatcgta
      841 aacttttaca agttcgatag tgccatttcc cagcaatttg ctgaatggtc ctcgacgatc
      901 ttctgattga gccaataata cccgacgaga acgcacatta ccgccatcta aaacagcagt
      961 atctgaaaaa ttatcttctt tgtcgtaaat aattttatcg aaaccgagta tatcgccata
     1021 gaatttgata gatcgctcaa tatcattcac gcccatcacg gctccataac tgcctccggt
     1081 agaaaatttt gttttaccaa accaatcatc gcccttaacc acttcaaata catttccata
     1141 cagatccttc aaataaaaat gaggattgcc tgcaggatca ttggtgacat cccccaacaa
     1201 attaattccg cgatttttca ggaaattgta agtagcctgc acatcgcgac ttttcatttt
     1261 acatgcataa attcccagat cgccatactg cacttgaaat gccggtgcta cgggttttcg
     1321 tgatgtgtat tgccatatct caaaacctcc gccaccgttc atattaatgg ccaatacagc
     1381 gtgacgttta tgaggcattc caccggtgta tggcaacatt aaattcgctt ctgcagcttc
     1441 ttcgaaaatt ttcacatcca taccgaagtt ctcgcgatac catcgccatg cttcatgcac
     1501 atatggattg ccaataccta cttgttgaat tcctgaaata atgggtttat ctgacataac
     1561 aactgaattc tgatcgatta ttttgtttta actctggtaa tgagataata atataaaccg
     1621 ggaataaatc ggcggaaata agtcattaga atttctttcc ctccgattaa tatttccttt
     1681 ttatttttct ccatgccacg tatcatctgc tgcacacatt tttctaccgg catgcctttt
     1741 tcttgtccgg gatccatcac gccttgagga ttgccatcac ccaacaccgc atttacgctg
     1801 atattggtat gaataaatcc cggtaccata atatgtatat ttatgggttg cttagcgatt
     1861 tttaattcag cccgcaaggc atcgaaataa ccaataatgg catgtttact tgcgcaatag
     1921 gtagttctgt tcggcacacc aatttttccg gcaacactac tcagcaacac gatacgtcct
     1981 tcacctcgtg caatcattcc cggcaatagc gctctcgtaa tagtaactac tgatgtgaaa
     2041 ttaatttgca tgatacgcgc ttcagcatcg ggaacagtgt tcagcatgct atcacgctga
     2101 ctgatgcctc cgctatgaat gatcacatcg atgcgaccgt gtatggcttc tgcgcgttta
     2161 gccatttcag gaagagcatt gttatcggat aaatcaagtg ataaaatgtc aacgcaattg
     2221 gtatgcacca aacattgttc tctcacgcgc tctaattcaa gtgtgcggcg agcagaaaga
     2281 atgagatagg cttttttcgt tgcaagttga tacgccagtt cctcgccaat tcctgaagaa
     2341 gcgccggtga tccatattac cttgcctgca aacatggcgg caaagatact taacgcccct
     2401 taggatgttt aatcatgcgg tgataagtat tgcggatggt aagcggacaa aacagtccgg
     2461 tcaaatacat gctcatggca acggcatttg cgatattcac ttttaaccaa ctattttcat
     2521 cgtacttcct cgcggaaacc atcgcctcat cgcgcagcac ttcaaagcga tgtcctttgc
     2581 tgatacgtct gaccatgtca taatcctcca tcacgatata agattcatca aatccaccca
     2641 atgcagaaaa tatgtccttt ttgatgaaaa gcgtctggtc accgcctcga aaaagcaaac
     2701 ccccgaagcg attgaaaaag ctgttgaatt gtaacatgcg cttattgctg tcgaagcggt
     2761 atcgaaaaca accggcgtct acccctttgt ttaaggcatt gcggatgaag gagctgaaat
     2821 tggcgggggg aaggcaatct gcatgcacaa agaaaagtat cgttcctttt gcaaatcgtg
     2881 ctgcaaagtt catttgctga gctcgccctc tttccggact ctgcagcaca ctgacaggaa
     2941 agtttgatgc aatactagtt gtatcatcgg tcgaccctcc atctgcaaca attatttcaa
     3001 atggcatgtc ataggtctgt ttgagcagaa attctaaggt attgccgatg aatcttgctt
     3061 cgttgaaagt tggaatgatg atgcttaaaa acatttataa tcttttgacc atcacataat
     3121 catcctgcag gtcgtcgcca agttgaaaag tggtggtgcc aatgatctca aaaccctgat
     3181 gtatgtaaat gttcacagct tttggatttt tctgccaaac tgccagccag atcaccttgc
     3241 aattattggc tttggcatgc tcaaaagctg actgcatcaa agctgcaccc actttccgac
     3301 cctggaaacc ggcatctaca tagtaacgcg acacctccat ggcaggattt tcgcttacat
     3361 acgaagaaac accttgactt agttttatgt atccaacacc tgaatcacct gaaaaagcaa
     3421 tgaagaatga aacagcttcg tcacccaatt cagcttctaa agcattgata ttgaagtttt
     3481 tagaaatata atcagtcatg ttttcgggag tgttgaaagc ctgatacgta tcaaaaaagg
     3541 ttttagcacc cagttttgcg agtaaagggg catctgcgga taaggcaaga cgaatagaaa
     3601 tgggctcctg atggggcatg acagggcaat tttaacgatt cgccctcaaa aagtgccgac
     3661 tcgtcgaaaa atttagttaa aaagtattgt atgcctgtaa ctttgtaacc ggttcatacg
     3721 tcatagcgtt aaattgcgtt aaagacgcat gagaaacatt aaaactcatt aatttcacgg
     3781 tgcaagaggc accacgaaaa acacgaatat gcgaaaactc cttaccatac tgttcgcgtt
     3841 ggttctttca ggacactttg cttccgcagc tactgccgac ggagaggacc tcattcgtaa
     3901 agcaactgcc gttattgatg aaacggtgca acattacgcc gccaaagagg gtgaaaagaa
     3961 agtttcagta agcacaattc tacccgatcc tgataaacaa aacgtgatca ttacctttac
     4021 cattgatgag ttgaaccgca ttcatgttgt ggaaatccag ggtggttacg catatcttac
     4081 ccattatatt aaatcatcgc tcgaagggca gaccattaaa tctgagcatg ccattcccgg
     4141 tattaattac atgatggcta ttaagcttcc tgcttctgcc tgattgcgat attggtattt
     4201 tccggttgtt ttttaccttg tttttttatt gaagtcaaaa ctgcatgacc ggttgctcat
     4261 cgcatttgcc attatcagcg cctgttgata tttcaaaagg cttgttaccc tgattcagcc
     4321 tgtaatctgc ggcaatattg ctatttttga acaagcattt ctttcagcaa gacatttgca
     4381 tatagtatat tattgattaa tgtttgctga aaacttgtgt tgtttaaaat tcaaaggcat
     4441 aacaataaat atttaatgaa gctcattagt atctcgtgta atttttttgc tctgatgttt
     4501 ttgatgcagg caatgaatct gcacgctcaa aatccttcct ggtcggcatc ggaaattcaa
     4561 ctggaaattg aaaaattaac ttcatcacat tcggtgcttt atattgctgc gcatccggat
     4621 gatgaaaata ccagactgat tacctggttg gcgaatgaga aaacagttcg cacaggttat
     4681 ttatcattga caagaggtga tggaggtcaa aatctggtag gcgaggaaca gggagcatat
     4741 ctcggggtca ttcgcaccca ggaattactt gctgctcgaa gaatggatgg tggcgaacaa
     4801 ttttttacgc gtgcagtaga ttttggatat agcaaaagtg catcagaaac ttttaccaaa
     4861 tggccacatg atagtttatt agccgatgta gtttgggtga ttcgcaattt tcaacccgat
     4921 gtgatcatca ccagattccc aaccgacgaa cgcgcaggtc acggtcacca cacagcctct
     4981 gccatgcttg cggaagaagc ttttgatgcg gctgctgatc caacaaaatt tcctgaccaa
     5041 ttgaaatatg tttcagtatg gcaggcaaag cgattatttt ggaataattc tacttggtgg
     5101 gataagcaat tgcctgaaaa aattgcgaaa gcagatccta cacttgctgt gatagatgtg
     5161 ggggggtata atgccttctt aggaaaaagc tatggtgaaa ttgctgctga aagtcgcacc
     5221 aatcacaaga gccaaggttt cggtagtgca ccgacacgcg gagaacaaaa agaatatctc
     5281 gaattgaaaa aaggcgaggc tttttcgaat aatgatattt tttccggatt acctattgat
     5341 tggtctgtat atgaaaataa ggtttcggca ttagcattag cttcatcagc ctttcaaaat
     5401 tttgatgcca agcatcctga tcgttcgata ccaatattga tagattacat taaaactctt
     5461 gagacgtttc cgcaagatcc catcgtaata agacggattc agcaagcaca gcaaattatc
     5521 ggagcatgta ttggattatt tttagagcct gcagcaacca cagaaaaagt ggcagccggt
     5581 gaatcactta ccattgtaag cactgccgta aagcgtcttg attatcctat tacattagag
     5641 tcgattgaaa tattgggaaa aacttacaaa gagggtgaaa tattacctac caatattaat
     5701 caaatcgaca cgatcatatt tgaagttccc gaaggcatca cctcctcgcc ttattggctg
     5761 gagaatgatt ataatacctt atttgatgtc agagatcgaa gcctcattgg taaagcagaa
     5821 aatgatcctg ctatttctgt gatctatcat gtgaagattg gtgaccgttc ttttgccttc
     5881 aagcgcggag tgatctataa ggaaacagat gctgttaaag gtgagattat tcaaccattg
     5941 actgttctgc caaaggcgct gattcgtgcc aataaatcgc tgttgatgtt cagatataat
     6001 gaacctctgt ttgtagatgt aattgtggag tcgaacatta aagaccttga tggttttgtg
     6061 tttgtaagta atgacgaggg atttattgta agcgggggac gtgagattcg tacattgcag
     6121 gccggtcaga caattgcggt acaattttct attcaagcac caactaacag caaggtagaa
     6181 acaggcaatt taaaattgta ttttatcccg ggcaaaaatg caaaggcaat atatgattcc
     6241 ttgatacatt ccggcgttta ccatgcagtt gagaattata ttgtaggcag taaaaaaggc
     6301 gtttatcgcg atttgcaaac aatcagctac gatcatattc caatccaaca gattttggag
     6361 catgctgaag tgcgcatggt acgcattcaa tcgcctgtgc caacattccc cgttgcatat
     6421 attgaaggag ccggcgacaa ggtggatgaa agtttgcgtg catttggcgt gcaggtcact
     6481 acaataaatc ctgaagacat tacacaggca ttattgcaac aatttcctgt tgtaattatt
     6541 ggcgtgaggg catataatac ctccaaagcg atggctgaaa agcaggcatt gttaatggat
     6601 tatgtacaag ccggaggaac tttgataaca cagtattcta ctaattggga ttcatacgtt
     6661 gatcaactcg gaccatatcc atttaagctt acacgcaaca gggttacaga tgaacaatct
     6721 ccggtgagtt ttttattgcc tgataatccg gcattgaata cacctaataa gattactgaa
     6781 aatgatttta aaggatgggt acaggagcga ggaatatatt ttgttggaga ggctgattca
     6841 tcttatgcat ttccactggg cttaactgat cccggagaga aggagcaaaa cggagcgctt
     6901 atggttgctc actatggcaa tggtgtgttc atttatacag gactggtatt tttccgcgaa
     6961 cttcctgcag gtgttccggg agcctatcgc ttatttgtga acctgatgag tatgcgtgga
     7021 aaatctcaac ctaaatgaca cccgaagaaa aaccattttt ctggaaggca ttatatatca
     7081 ctgtagtcat tgtgctactg gtggaaatat ttatttttta tagcctcagt caattattcc
     7141 catgagtact atcgactggt tggtgatgtt cgggacactg ttgttgatag cagtgtatgg
     7201 tatctatcgc acgcgacaaa ttcatactgc agaaacctat ttgcgtggcg ataatgaaaa
     7261 aagatggtgg gagataggac ttagtgtaat ggctacgcaa gcgagtgcca tcacatttct
     7321 ttctgttccg ggacaagctt atgataatgg tttagggttt attcaatttt atctcgggct
     7381 tccactcgcc atgattgtga tatccgtatt ttttataccg atattttatc gattaaaagt
     7441 atatactgct tatgaatttc ttgaaaacag gtttgatgta aaatccagaa cgttaacggc
     7501 tgtgttgttt cttgtgcagc gagcattttc tacaggtatc accatttatg cgccatctat
     7561 cattctttcg caattgttgg gatggccttt gacctcaacc gttattttta ttggtggaat
     7621 tgttaccctt tatattttaa gtggaggaaa taaggcagtt accgttaccc ataaacaaca
     7681 aatgctggtg atttatgcgg gtatgtttac tgcctttgga tttttggcat attacattac
     7741 gcgtgatata aatttaggtg aaggaattag ccttgccggt tatatgggca agatgcatgt
     7801 cattacaaca ggcagcgaat caggtcattt tgattggagc gacaggtata atattgtttc
     7861 gggattaata ggtggtttat ttcttcaact aagttatttc ggcaccgatc aaagtcaggt
     7921 ggggcgatac attggaggtg catcggtacg cgagagcaga cttggattac tgtttaacgg
     7981 gctcgtgaaa attccggtgc agttttttat tttatttatc ggcttattgg tttttacgtt
     8041 ttatcaactc aatacgccac ctgtttattt taatgatgtg gcatatcaaa aggcattgca
     8101 ttcggattat ggtccgcagc ttgaaaaact ggcaagtgac tatcaacttg cttctgaact
     8161 gcaacaatca aatgcgcgtc agactgtaaa taggaaggag caaacgatat atgaatatca
     8221 ggaatccggt cgcaaagctg atagtattcg actcgaagtg cagaaaattg ttgaaaaggt
     8281 ggagccttct acgcaagatg accatgatta tattttcatg cgatttgtga tggatcacct
     8341 tccggttggt gtgataggat tattgtttgc agttattttt tgtgccagta tgagttcaac
     8401 tgcttcggct ttaaatgggc tgtctactac tttttcggtg gatatttata aaagatttat
     8461 gtttaaaaat gctacaggta aacaacattt gcaagccagt aaaatgctca ctgcagcatt
     8521 tggaatttta gcaataatgt tcgcactcat cgtatctatg ttcgacaatc ttattgaggc
     8581 ggttaatatc cttggtagtc tgttttatgg cactatcctt ggcatttttc tcacagcgtt
     8641 tttattaaaa cgagtaggcg gaaatgcggt gttcgttgca gccgttatca gcgaattggt
     8701 gatattgtat attgacttca gcaccagata tcattggaat acgcctacca ttcaaatcgg
     8761 ttacctttgg tataatgtaa taggttgttt gctggtgatg ggaatttcat tggtactcag
     8821 ttttatctta ccgaagtatg aaattatttc tgaaggaatt gatttgccgg agtaataata
     8881 gagtaatttg taagccaagc cttaatgccg atgccttttg gtttgtgcat tgtatgttga
     8941 tgcaaatcaa aaatcgttaa atggcggcct aaaaatcggt aattttgcag catgaggcaa
     9001 taccacgcat tacttcagcg tattctggat gaggggacaa ggaaatcaga ccgtaccggt
     9061 accggcaccg tgagcatttt cggccaccag atgcgtttcg atcttcagga agggtttcca
     9121 ctggtaacta ccaaaaaact gcacctgcgc agtattatcc atgaattgct gtggttttta
     9181 aaaggcgatg gcaatattgc ttatctgaag gaaaatggcg taagcatatg ggatgaatgg
     9241 gccgatgaac atggtaacct cggtcctgtt tatggtgttc agtggcgtag ctggcctact
     9301 gctgatggcc gccatatcga tcagatcagt aacctcatca accaaataaa aaagagtccc
     9361 gattcacgaa gattgattgt tagtgcctgg aatgtcgccg atatagaaaa tatggcattg
     9421 cccccttgcc attgtatgtt ccaattttat gttgccgatg gtaaattgag ttgtcagtta
     9481 tatcagcgtt cggcagatac gtttttaggt gtacctttta atattgccag ttatgctttg
     9541 ttgacgatga tggtagctca ggtatgtgat ttgcaatacg gcgagttcat tcatactttc
     9601 ggcgacgcgc atttatataa tgatcatatt gaacaggcaa aattgcaatt atcgagagag
     9661 ccacgtgtac tgccgcaaat gaaaattaat cctgatgtga aagatatttt cggattcaaa
     9721 tttgaagatt ttgaattggt gaattatgat ccacatccgc atatcaaagc aaaagtgagc
     9781 gtttaaaaaa gtatcatgcc tttattttcc attattgtag ctgcctccga aaatgaggtc
     9841 attggcatgc aaggcgatat gccatggtta aagcttacct ccgatctgaa atacttcaaa
     9901 gagaaaactt ccggccattg gtgtattctt ggtcgcaaaa cgtataatgc cctcggcaat
     9961 aaagtgcttc ctaatcgaaa attcatcatc gttacccgcg acaggaattt caagtcagat
    10021 gacagcatcg tagtgcatga tctgaatgaa gcaaggcatc accctttgat tcaacaggag
    10081 gaagaagtgt ttatccttgg aggcggtgat atctatcgtc aggcgatgtc gtttgtagat
    10141 aggatatatt taacccgcat tcatgcaagt tttgaaggtg atacctattt tgccaccccc
    10201 ggcgaagggt ttcatctggc ctcaaaagat gtaagaacga tagatgaaaa aaatccttat
    10261 gatcacgatt ttcttgtcta tgagaaaaat tagataattt catcgccgca atgaacaata
    10321 accagcggcg tactaccatc ttcaacctta ccgtgctcgt agcagcattg ggttactttg
    10381 ttgatattta tgacctacag ctctttaatc tggtaagtaa aaccagtctt aaaggaattg
    10441 gcattactga tctttctctg gttgattatt gggatgtgcg attattcaat gtgcaaatga
    10501 taggcatgtt ggttggcggt atcatctggg gcattatggg cgatcttaag ggaagaaaat
    10561 caattctttt cggaagtatt ttattgtatt cggcagcaaa tattttaaac ggatttgtcg
    10621 acaatactac gcaatatcaa gtcgttcgtt tttttgcagg gttaggcctt gcgggtgaat
    10681 tgggagcggc cattacactt gttgcggaaa tcatgagtaa agagcacaga ggttatggaa
    10741 ccatgttgat tgtgagtgtt ggtgcattag gagcagtagc cgcttttttt attacctctc
    10801 atgtcgattg gcgtgtatct tattatattg gcggcggact tggtttggca ttgctgttgc
    10861 tgcgattcgg tacattcgaa agtacgatgt ttgatgaggt gaaacaacag aatgtagcac
    10921 gaggtcaatt cctctctttg ttcagcgata gaaagcgttt tttgaaatat gtttactgca
    10981 ttttgatagg aattccggta tggtattgca ttggtgtatt aatgaaattc agcgagcgat
    11041 ttgcaaaaga gtgggggatt gaaatgacac cggaagaagc tattcaggta aggcgaacag
    11101 ctatcatgtt aagttatatc ggcctttgtg taggtgattt attgagtggt tatttaagcc
    11161 aggtatttaa gagccgcaaa aagatcgtta tgggatattt gatagcaact gttgtattag
    11221 cattggttta tctgaatatg aaaagcagtt ctcttacatt attccatatt atgtgtttct
    11281 tattgggatg tgctaccgga tattgggcat tatttgtaac catcgcatca gaacaatttg
    11341 gcaccaatat cagagctaca gtaactacta cagtgccgaa cttcgtccgt ggattagttg
    11401 taccacttac actgagtttc gcaggtttga gcgtttcggt cggtaatata cagtctgcaa
    11461 tggttgtagg agcagtatct attggtattg ctatgattgc caccttatca ataaaagaga
    11521 catttggcaa agaccttaac tatatggagc tgagctgaat tccatcactt gttagtttag
    11581 tccctgcgtt tgtttgtttc ccgaaaatcg ggtttttgag gcatttactt ttggtttcga
    11641 aagcggaatc atccgcactt tttacccaaa cttaaaatgc taaatatgcg aacacttaaa
    11701 cacttatttc tgacaggcat acttctgcca ctcagttttg cctatgcaaa ggcttgcgat
    11761 tattctcatt tcgatttagt gtcaactact gatctcggtg gaggccaata tgaatttgta
    11821 gttaccttct gcgcaggtgg aggtgaaggt ggtgccgatc aaagtacgta tacatgggga
    11881 gtcgaattgg ttggacctgc aaccttttct tcatggcctg caacacttgc cagtccacaa
    11941 acaggggcta tctatgctgc agacggatct ttctattatg gttctaatta ccttatttat
    12001 tttcttgatt catatactgg aaccggtttt gcagcgcaaa gttggacaac agcgagcgct
    12061 ggttatggtc ccgcaggttc gtattgcaaa acgtttacaa tagtaaccaa tggtatgcca
    12121 acacaactgc gattaatggg tgctgaagct gcaggtattg ctgttgaacc ttatggttgt
    12181 aatggtgctc ctgacatgga aataaatttt gatgtggctg ctgatgcggg tctcacaacc
    12241 tcgttatgtg caggaaatac cgtaacatta aacgctactg caacaggtgg aacgcctcca
    12301 tattcatttg tgtggaatac cggtgctaca acagcatcaa taaatgtaag tccaacagtg
    12361 aacactactt atactgttac cgttactgat gcaaatggac atactgattc agatccggtt
    12421 gttgttgttg tgcacccaaa acctaccgtt aacgcaggta ctgacaaaac aattacgatt
    12481 ggttatggag caacttgtgc tactttaacc ggcacagcaa caggtggctc atcgccatat
    12541 acttatagct ggactggcgg tggaacttca ttgacaaaaa ctgtttgccc aactacaact
    12601 acaacttaca cactcactgc aacagattat tatggatgca gcaactcaga tgcagctctt
    12661 gtgaccgtga aagatgttcg ttgcggacct tcacttacaa aagtgtatgt atgtaaaatg
    12721 ggaacaacca agtgtatcac tacaggccag gtgccaagtt atctcaccag cggttgggtt
    12781 ttgggagcat gctggatgaa gcttgatgac gaagtagcac tcagcgaaaa tcctatcgat
    12841 attttcccta accctgctac tacatctgct gaaatcgtat ttgtgatgac tgaagatggc
    12901 gaagcaacta tcgaattata tagccttaat ggtgaacgct tcccaatcgc agatgcagta
    12961 gttgccggac gtgcaggtga agagatttca catgtaatcg ctctcgacaa tatacctgcc
    13021 ggtatctatc agatatttgt acatacaaca agtggacaga tcatgacgga aaaattggct
    13081 gtcatccgtt gatcctctgt tcatttataa caaaaacctc caaattgggg gtttttgttt
    13141 ttttatatac ctgcattaat gactttcaac aaatgctttg aattgttcca tgtatttgta
    13201 agattgcttt ttaaaagcac cgggcatcat gaagcctatt aatttcataa atccggaaaa
    13261 gtgaaatgtg ttttcggtag tatataaggt agttccattt cctaaatcct tgaacttatt
    13321 tacactgata tttacaacgc cctgagcttc ataagtgcct gaaaattcag ccggaagatc
    13381 gcgcaccgtg atagtttcta tcatttccat gcggcgcttg ccatttagaa aaaccaattt
    13441 cgatttggca ccgggctcac cggcagttcc acttatcagt tcaaaagatt gtaacccact
    13501 catccactta tacagattat caggattatc gaataaacga acaacctcgt caacaggttt
    13561 gttaatagtg atttgataag tatagtacat acgatttctt tgtaagtgaa gatacagcat
    13621 ttccttttta cgaaagcgta tttcgtcgaa ctatacaaat taccctttaa tttagtgttc
    13681 cagggcctta agcaccagat cgcgtttgcg ttgggaaaca ggaatttgat tgccatcctc
    13741 caataccagc atacctcctt ccgatttatc aaacattttt atcttttcgg gatttaccag
    13801 aaaggaatga tgcgttctga cgaaaccaag cggctcgagc atgctttcat attccttcag
    13861 attttttgaa accatgatct gctgattacc cataaggttg aacttggtat aaatgccttc
    13921 cgcctctaaa aacaaaattt cttcaatacg cacataataa gttttgtgca tatcctttaa
    13981 gatgattttt ttgggaggct cgtgttttga tttcacttgt tctaataaaa aagccatttg
    14041 cttactcggg tcgctctgaa tattcttgat tgctcttcct acactatgca tcaagtcatc
    14101 cggatctatc ggttttagta aaaaatccat tgcactgaac cggaatgcat tgatggcata
    14161 cttatcatga gctgttatga aaattaattg gaaggtatat gccgggagtt gacttaataa
    14221 atcaaaaccg gtgccttcat ccaattctac atccagaaat aatatatcag gattaaacca
    14281 acctatggta tcaatgccgg ttttgatatc tgatgcttcc ttgatatgcg tgatttcagg
    14341 acaataagct agtaacattt catgtaatgc tactctaatc ggtgtttcat tatcaattat
    14401 taagactttc atcttcagta atttttggta atcgtattac aacctgcaca cctttgccat
    14461 cgcgttgatt ggcaatttgg aaacttgcat tagtttttaa cttgagattg agcaagtaaa
    14521 tcctgtctct gataatctga ctggctctgg aaatatgttc attcgtaatt tccttttctc
    14581 cggcaaggcc tttcccattg tcaagaatgg aaacaacaat gttatccccg tcttcatcaa
    14641 acaatatggt tatttcacca acataatcaa ttcccctgaa tccatgctct attgaatttt
    14701 ctacaaacgg ttgaataatc atacttggaa tcagtaaact gtcgggttcc gataatgcat
    14761 ctgccgacat gacgaatttg aatgcctgag ggaacctgat ttgctgcaat gtgagatatt
    14821 cataaagaaa atcgatttcc tgctgaatgg taacataatc tttgtaggtt gattccaatg
    14881 tttcacgcat gatatgactg aacttcgata agttactcgc caatgccttt ccatcatttt
    14941 cacgcaatgc aaatccctgt aatgacgcaa gtgcattaaa aaagaaatgc ggattcatgc
    15001 gcgcgcgatt gagtcgttgc tctgtttcaa gaatcagttt tttattgttt aatgtttttt
    15061 gacggtaata caggaatatt gaaagactta caagcaatgt cacaaggatg ccggcagcta
    15121 gtatccttat ctgcaaagtg ctgatttgtt tttccttgct gagttcactg atcttttttt
    15181 cgttttgaac tttatcatac ttttgttcta actcggtcaa tgcagaggtg ttttcaatat
    15241 ttaccaaact atctcgaact gctacatgct gctcatagta atacaatgcc gaatctgact
    15301 tccccaggtc tcgataggta aagtacaatt gttgattgat aagactcact atgccttgac
    15361 tcttaaattt cttagcgttg cccagcgctg catgtaagct tgccaaggct tcttcaacac
    15421 gtcccagctg caataaacac tgacctcttc gatattgtgc cgatatctgc aattgttcgg
    15481 cacccgcagg aggcaacatg gccataacgg tatcattata tgctaacgcg gttttataat
    15541 ctcctttttc gatggcaatg ccacctaatg atgtataccc gcgaacgatg taatagggat
    15601 ctttggatag ttgtgcatat ttgatggaaa ttggtgcata catttccatg gaatccagat
    15661 atgccttgtt atgtgtgttc tcatgaaaca taccataagt tgccaggata tttgcataaa
    15721 gcatcgcaca taatttgtcg tcttttattt gctctgcgat ggcagcacct tcctttaaaa
    15781 actttaatga aggttccggt ttgttcatac tgccataagt atggctcaat tcattgcaaa
    15841 atcgagcagc atatttaaga ttctgatgtt ttttcgactg ttcaatacct tctgcataat
    15901 agcttgcagc tttatccaaa tcggccttac ctgctgctac agcccctcgt aaacgaagta
    15961 tttcaggaga aacaagggtg tcataattgt tgccgattag tttttctgct ctgtcaacaa
    16021 acttaccaat gctgtcaaat tgtttatcta tcaaaaaagt attggctaca agtatgtttc
    16081 ctgcagcttc gcagcaccct ttttgtttta tcagcgtatt gctcagtaag cgtgcactat
    16141 ccagtttccg catagaaaat aatgcctcat attgatcgcg aaacgattta ttatacgatg
    16201 cacatgaaca atcgtgttga gcttttgtaa gcagactgct acaaaccaat aatagtagca
    16261 gcaagccctt taattgaaat tgattaaaca tagaaaaaat gtgtggtata aaattatcta
    16321 ttcagataga ataaacagtt tcgcaccatg taagaatggc gatatcaaca atcaattatt
    16381 attcgattgt ttgcacttat cagatgaata tcctcacttg ttgccaaaag cgagaattgt
    16441 taaagatttg taaacattcg agagcacgga tgcattgtaa atacggaatt cccggtagtt
    16501 tttgcgccgt tttattactt cccaacggtt atcatggatt tgccttcact tatcctaaac
    16561 agctcttctg actcaatgaa agcactaatt ttggctttcc ctgttcatta tatatatgct
    16621 ctgttatcct gactaggaag cacacagtta catttaatac ccctgagccc cattttgttt
    16681 cgcagaaaaa aagccattcg tctgaatggc tttttgtatt ataaactatt tagtttctcc
    16741 agttcatcca gaaatgcgct tttctttcgc atggaaacag ggacttcact catgtcgctc
    16801 atcatgacca ttcctccatc tttgtttctg aaagatttca catggtttaa attgataaga
    16861 tgtgaatgat gcacacgctc aaaaccataa tgttgcagag attcttcaat ttcttttaga
    16921 atctttgcta ataatatttt actgccatct ttaaggaaca cgaagctgta attgccataa
    16981 ctattgcatc tgatgatatc tccaacaggt atcacatgca gcccttcggt agtatgaaga
    17041 ataattttct tttgttgcaa agcattcaaa tcgcgcctgg cagaattggc aacttgttgt
    17101 aaatgtttct cttccttcgc atttttcacc ttgttgacag ctgcaaccaa ctcctcacta
    17161 tcgatgggct ttaataaata atctacggca ctggtcttaa acgctttcag tgcatattct
    17221 gagtatgctg tagtaaatat gatggcatag tcatcatttt ctatttgctc gagcactgta
    17281 aatccaaggc catctgacaa ttgtatatcc aggaaaataa tatcaggatc tgccttttcc
    17341 agcagcgaca ctgaggcgga gatactgtcc gcttcaccaa taatatgtac atcagggcaa
    17401 ttaagtgtaa tctcctgttt tagggctgag cgcgctctcg attcgtcgtc gataatgatt
    17461 gcttttaaca ttactgtatg gtttatgggt ttaatatgat cagcggcata ctcaacctta
    17521 tgcgtgtgcc gatgccttta cctgtttcat ctgtcagatc gtcgatatcc agaataaagg
    17581 cttccgctct ttcgcgttgg tttagcgcct gtaaataatc attgataatt tgtgttgcct
    17641 tgctattgtg ttcactgatc ctcgaccttg ttgcagatgc ctttcttcca atgccatcgt
    17701 cttccacaat gcagatcaat ttattatcgg aaacactgaa gtcaatggcg atatgtccac
    17761 tgccttgctt gggtgctata ccgtgaacaa gtgcattctc aataaatggt tgaagcagca
    17821 ttggtggaat gccggtccgc ataacttgta gtggttgatg cgcgctgatg ttgtaagtaa
    17881 acttatatgc ataacgcatt gcagccagat cgagatagag tgttattgca ttaatttctc
    17941 gctcaatcgg tatccactgc tcattatttt ctagaatatt ccgtaataat ttagagaatt
    18001 tagagatata atcgctggct ttcttgatat cattttctgc ataataccct ttgatcgtat
    18061 tcagggcatt gaaaataaaa tgcgggttca tttgttgcct gaatacctgt tgattcaact
    18121 gttcaatttt ccgctccgct tctgcatgtt ccatttccag ttgaatagta cgctgcttca
    18181 ctgtaaagga ttgttttact gcacgaagac gcattcgcca tccgattaaa ataattcctg
    18241 caagtgcaca aacacttaaa atgataaacc acacctcccc ccaaaccggt ttgtttatgg
    18301 aaaaagatat tgtgcttaca ggactagaaa ttcctgcaat attggtagcc tttacaagga
    18361 atttgtaatc tcccggagga agggatttta attgaatgct tctatctgta gtgttgtgcc
    18421 attttgtatc ataaccactt aaatagtaag tgtaagtaat gcggttttta ctcgtaaatg
    18481 acatcccgga atattgtaca gtaatgtcat tttgtttcca atgcaatcta acatctttcc
    18541 caagttcaga ctctttgccg ttaacctgaa aagagctgat tattattctc ggcggcacag
    18601 gatttggcaa ctctatgttt tgatcaaagg caattaatcc gctttctgta gaaatgaata
    18661 ctttcccttg tgctacatga acatctgtga tcctggaagg gtcaaggcgc aaagccttag
    18721 tatagttaat agttgtgaaa ttttcaccgt tttttgtgat ggcaactaat tgatttccaa
    18781 aggcagccca aattattccc tccttgtcaa gaaatatggc attacagtcg ctattaggta
    18841 agccggagtt tttatctatt atggttacta attgctcgtc gcgtaaaacc aataaaccat
    18901 tcatggtacc cacaaactgc aatccggttc tagaatcaaa aagaatgtct cggatcgggt
    18961 ttttacacgc agcaatgtga tcgcctacaa agctggtttt accattttca taaatgtaaa
    19021 gccctctgga agtgcctatc caaatggcat tgttgatttg acaaaataca tttgttcttg
    19081 cattgattac ttcataatct ctttttcgct cagggtagtt aaaaaccaat tgttttattc
    19141 ttgtttcaaa ctcatctctt gtaaaaagca tagcattatc ctgcccaatc atgaccatgt
    19201 cattataaaa aatgaggtca ttggcatata caccatatga tttattaaat cctttgccca
    19261 tacccaaaat tcctgctttg ccaactacat atgttctatc accaaaatgt cgaatattca
    19321 tgatatccat aggcgtcggt tccggtaacc tcctatctgc aattattttt ccattttcca
    19381 taatggcata atggttattg gcacaaccta tccataatcg accgtctgca tccgcttcca
    19441 atgccgttat tttatcttca gaaagaccgt aatcgctatt ccaaaaggag acatttacat
    19501 ttggcatcag gtatacaccg ctttgtaatg tgcttatcca ggtgttgcct tcgaaatctc
    19561 tttctgccga tgatacagaa tagcccggca ttagatattt gtaatctttt gtaatcctgt
    19621 tccaaatgaa taatccgttg cgcgtgccta taaatacttc ttcatagttg atgaatttaa
    19681 taaatattat ttcggcactt ccttgcggca acgtaaactc cttttcaaga atgttgtcat
    19741 ctttcagact gtatacgtct tgctcattac taatcaaatg catacttccg tatgtatctg
    19801 cccgaattat tcttgttgga aaggaagctt tatccatcat cttgagcgag ccgtcctttg
    19861 ctacccagat gaaattctca agtccgctca ccgatatctg tgaaacttca tcacggtcat
    19921 ttattttata cactaccgga ccccttcctg ccaaatataa attacctctg tcatcttcac
    19981 attcggccaa aaccattttg ttgaattcca attgctttac cagcgaatcg ttttgtgcat
    20041 tgtgaatgct gccattcaaa taaaatgatg gatttccatt gataggctgg aaccaaattc
    20101 tattcttttt atcctgataa atgcgcaaaa tttcattatc acctagccca tcggcactgg
    20161 tgaaatgggt aaacgcatat ccgtcataac gataaacacc actatttgtg gctatccaca
    20221 ttacatcaga gttatctctg aaaacataaa atgagttggt cggtaaataa tcatcagcag
    20281 gtgttacagg aacgatatac ggatgttgcc caactagcga taaatgccat tgcgtcaaca
    20341 ctacaaacaa tagtgcatac aacagctctt ttaccactaa ctgctttgtg cgttttaatt
    20401 gtcggaaccc tgctttcaat gttataacta ttgcaggcta taaaaataag agaatactgt
    20461 catattaccc cctgcaaata tttcagttat taccctttgg caaatcactt attcggtaaa
    20521 agtcaacact tatatgccgg gttattgcag ttgtcgctaa aatgttatca ctttgatatt
    20581 tctagctaat tttcgacaat ggcaatcaat gcagattatc ggacacaaaa aagcccgata
    20641 catgctatcg ggctttagaa gtatatggtt gattgattaa tatgctcttg caaataatac
    20701 gcgctgtgtg cttggttttc cggaatacac acaatttccg gtttcctgca cggcatccag
    20761 ggggatacaa cgaatagttg ccttagtttc ttccttgatc ttttcttccg tttctgaagt
    20821 gccgtcccaa tgcgcactta caaaaccacc gatttcgatt tgtttcttga aatcatccca
    20881 actgtcaact tcatgcgtgc ttgcggtgcg gaattgcaat gcgcggttat ataagttctg
    20941 ttgaatatct tccaacaatt gttgaatata taaaccaaga ttttcattct tcactgtcac
    21001 cttttccttg gtatcgcgac gtgcaatttc caccgtacca ttttctacat cgcgaccacc
    21061 catggctatg cgcacaggaa cacctttcaa ttcgtattca gcgaatttcc atcccggctt
    21121 tgcctgatca ttgtcatcgt atttcacggt gatctttaga tcttttaatt ccttcatgat
    21181 gccttcggcg atggcagtaa tagcggcgag ttgttcttca gttttataaa ttggcactat
    21241 caccacatgc aaaggagcca ggcgaggtgg aatcactaaa ccttcatcat cgctatgtgc
    21301 catgatgagt gcacccatca tacgcgttga tacaccccaa ctggtggccc atgcgtattc
    21361 ctgttttcct tcgcgcgtta aaaatttcac atcaaatgct ttcgcaaaat tttgtcccaa
    21421 aaagtgcgaa gtaccacatt gcaacgcttt accatcctgc atcaagcctt ctacacaata
    21481 tgtatctacg gctcctgcaa agcgctcatt tgccgttttc acacccttga taacaggaac
    21541 tgccatgtag ttttccatga aatcagcata cacattgagc attttcacgg tttcctcaac
    21601 cgcttcctcg cttgtagcat gagctgtatg gccttcctgc caatgaaact cggcagtcct
    21661 caaaaacaga cgggtcctca tttcccaacg cataacattt gcccattggt tgatgaggat
    21721 aggcaggtcg cgatagctct gaatccagcc tttatagcag ttccagatga tcgtttcaga
    21781 ggtaggcctg atgataagtt cctcttccag gcggctttca gggtccacca tcagcttgcc
    21841 tttcttatcg ggattggttt tcaggcgata atgcgttact acggcacatt ctttggcaaa
    21901 accttcagca tgtccctctt cttgttcaag gaagcctttc ggtatcaaaa gtggaaaact
    21961 ggcattcacg tgacctgttt ccttaaattt tgtgtccaaa acggtcttca tattctccca
    22021 aatcgcccat ccatagggct ttataaccat acaacccctc acggcagcat actctgccag
    22081 gccactttgg ataaccaaat cattatacca ctcggaataa ttaacacctc tttttgtaat
    22141 cttttcggcc atattgtatt tttggtatga tttatgtttt atatgattcg caaaggtaaa
    22201 gattcccttt gcagcattct ataaaccttt taaaatatcg attatgaaac tgaactacct
    22261 gatcatgctg gccgccaccg gattgatggc tgcatcctgc gccacacaga cagcatctag
    22321 aggtgcttca tctgacgata tctactactc agcaggtgat cctacctaca ctgaggagag
    22381 cattaaaacc gaaaccacca cttcaagttc taattactcc acctatcccg gcacttccgc
    22441 ccaaactgaa tatgcttatt cagaaccaca ggacgatgcc ggctatcgca tgggtggcga
    22501 tgacctgtct tcttcaaatg agtattatga tgatgaagga aacacttaca tcgtaaataa
    22561 ctattacgac gattatgact atgatgatta ctattataca tctagtcttt atcgttttta
    22621 ttctccgtat tacggatttg gttaccactc acattgctat tctccatact attatgatcc
    22681 tttctgggga ccatcttatg gttggaatgt aggttatagc tggggctggg gcggcattta
    22741 ttatggcagc tactatcctt attatcctta taattacggt tattgctaca acccttatgg
    22801 atattatggc tattacgatc catggtataa tccttactac ggttatggcg gatactataa
    22861 cggatataac aacggatatt acaacggtta ctggaatggt tactatgatg gctattatga
    22921 taatggctat tacagtggaa actatggtgg ctactacgga aacagcgacg attattacta
    22981 cggtgtaaac acaggaaccg gttcaaatac caataccggc gatccttctg caaacatcct
    23041 ttccgcaggt tatgataaag ccggcaatgg tactactgat atgaaccata tccttaccga
    23101 taaaggatca ctggtggttg ataaaggtga tgtgaacatg actgccgcag cagcaaatga
    23161 taataacgtt gcaggtgttg cagagttgcc gtcaatcaaa gacaaaacat tatctgatca
    23221 ttctgttgtt gctgccatca atgaacccgt tgcattaaat agctcatcaa caggtgtcac
    23281 tgaaggaaca actcgcactg atgcaggtgt tcgcgtaatt aaatattaca gtgctgatgc
    23341 gcaaaatgca agtgtgcaaa ctgatcgccc acaacctgta gtttcagata gaggtccacg
    23401 cggtgattat ggaacaagtg taaacaaacc aaccacaaca ggtaataata ctgtaactaa
    23461 cggagtgaaa aataatacca cttcacgtcc tgaatatcag caaacacctt cacgtgagcc
    23521 ggtttataat aataatcaga cacaacgtcc tgatcaacca tcatacgatc gtccgtcaca
    23581 aactaaacct agctacgaac aaccgaagca gccaaagcag ccaacatatg agcaaccgcg
    23641 caacaatacg ccttcacgct caaatgatgc gccgtctaag ccttcaaaaa gctatgatac
    23701 accaaaaagc aattccggca gcagctcttc acgtccgtca tataacagcg gaagcagcag
    23761 tcgccccagc aacagtggat catcaggttc aaagccgggt ggaagttctc cgaataaacg
    23821 ttccggaggt aattgatctg catttttaat caactctaaa ataaactaac catgcgcaag
    23881 ctaattataa tggttatagc tactgctttg tctgtaagtt cctttgcaca aagtgatgta
    23941 gatgctcttc gctattccat gctcgatttc ggtggaacag caagatcgct cggatcagga
    24001 aatgcatacg gtgcgctggg tggcgacatg agtgttacct ccatgaaccc tgcaggtctc
    24061 gctatatatc gcagctcaga actgaccatc acaccgggat tgctcagcat taattctgat
    24121 gctacgtatt atggacaatc aaccaccgat gatcgctatc attttcaatt gaataacgtc
    24181 ggacttgtat tcgcaaatgt gaatgatgga aaagcaaatg ctactgatgg ctgggtaggt
    24241 ggtgcatttg cacttactta taataaagtt gcgaattata acaatgctgt tacttacagc
    24301 ggatttaatg atggcagttc attgttaaat acatatgccg attttctaaa tagcggcgga
    24361 ggcacaaatc cgggagatgt tttcgataaa gatccgtttg gagccggttt ggcttgggaa
    24421 acatatttat tgaatccact gggaaccgat tcaacacaat attatccatt gatcaccgac
    24481 ggcaatgtgc agcaaacaaa agtgatgcag actgatggtg gcatgggtga atttgatatt
    24541 tcgtttgcag gaaattatgg caatagattg tatatcggag caaccatagg aataccacat
    24601 atccgctacg tgcaaagcta cacctggaag gaagaagata tcaataacga atacaatgat
    24661 ttcaattctt attcgcttgt tgatgtcact gaaacctacg gaacaggtat caatgctaaa
    24721 ttcggactta tctatcgctt aaacgattat ctgcgattag gcggtgctgt gcatacacca
    24781 actttgtttg gtatgtcgga taattattat tcaagcatgt cgagcgacct caattcaggt
    24841 gattattcat atgattctcc aattggtgaa tataactacg aactcgtaac accatggcgc
    24901 gtgatcggta gtgcagcggt tacactgaaa aacatcggct ttatttctgc tgaatatgaa
    24961 tttgttgatt acagtgaatc attcttcaat ttcaatcgca cactagatgc cggcgacttg
    25021 agttatgaaa caacagtaaa cacgaatatc gacacaaaat atggtgcatc tagcaatatt
    25081 cgtctcggtg ccgaattaat gtatgatgta tttcgttttc gcgcaggata tatcatgagt
    25141 gctacacctt tcaatgatgg cattgctgcc gaaggtgcag accttgcgaa aaatacttat
    25201 actgccggta ttggtataaa agataaatca tattttattg acctcgcctt cgcacatagc
    25261 aatatcaagc aatatgatat tcaatatgtg tatgacaatg gttccggcgt aaatgacgga
    25321 gctgtcatta atacatcgat aaacaatttt ctgttaagtt ttggttttag gttttaatga
    25381 ttgtcagtga agacaggccc tcctggtggt tcgggagggc tatttttttt aggagagagg
    25441 agaaaggaga ttgaagagaa gatgcttggg tgagcttgcc tcgccgagca ggcgggtggg
    25501 ggagtttacg tgactcgtga ttcgtgactc gtgaattttt ttttacttga tacctgatac
    25561 caatttcaca atcgcaaccc tcaaaaccaa tttaaccaca atagccacga agaggaacac
    25621 taagtggcac agagtgaatc caaactcacc cactgaccca ctcatccact gatcattcaa
    25681 ccacaaagaa cacggagagg aacacagagt agcatagagt aaatgcaaag cacattccac
    25741 cgcatttgtc tcactactca ctacccacct gcgacgaggc aggcccacta ctcactacca
    25801 aatcgtgaac catgaaattg cctcgctgct ggtatatcgt gaattttata cttgcacagc
    25861 ccttcgattt ttcggctctt caaatctcta tttgaacctg agaattcatt aacggttttc
    25921 cgtgaatatg gattacaatg agagaaaatt aacatta
//
LOCUS       DAOQHY010000022        25876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_832_length_25876_cov_5.567910, whole genome shotgun sequence.
ACCESSION   DAOQHY010000022 DAOQHY010000000
VERSION     DAOQHY010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 25876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25876
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_832_length_25876_cov_5.567910"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="PLL28_03235"
     CDS             complement(<1..935)
                     /locus_tag="PLL28_03235"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HNF68359.1"
                     /translation="MNSPNKEFHKSVEQQNDQSFNWRLFLTISWINRWWIVLVFAIGV
                     CAAFLYNRYATPIYESHADIQFKDKSKDNTIDLGIRVANSNIDRMNEEMTVLSSKGYK
                     ITALSDLPIGVSYYIRERVKAAEIYNESPFTVNIAILDSAIIGDKIDVQILDTSEYKL
                     SYTYKNEKFSKKFSFNKAYSTPAFNINTTLNTFAKSKFKFEYFFTINDLLKESERIQE
                     DVSIDVENLYGGEISIKYRDKNRQKTQDVVNGIADEIVKMSLHRKAESAKTVIGFIKD
                     QIDSIEIQLFNQETLLKNFKQNNQLISPEIAETNIV"
     gene            complement(939..1733)
                     /locus_tag="PLL28_03240"
     CDS             complement(939..1733)
                     /locus_tag="PLL28_03240"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNF68360.1"
                     /translation="MTSFSKQPTKRPIIHLLLAGMLLATFSSCGMLYPNRMLDTGKDF
                     VESSFLDTIYREFVIEPGDQIQVFVYPNGGYNLIESQISNQMNYYQPQTMLQSLSYQI
                     DGSGMANLPRLGWMKISGLTESGAEKMLTTEYGKIYTDAFVNVVITGKYITVYRGGSD
                     AKQLALNRSDLTLLEAIGMAGGIPENGRSSKIKVIRNIQGAAQLQEIDLSSIDDIALG
                     NMYIQPNDIIYIEPNINSTVFQEIGPILSTISSIAVIYAFFANVNQ"
     gene            complement(1817..3742)
                     /locus_tag="PLL28_03245"
     CDS             complement(1817..3742)
                     /locus_tag="PLL28_03245"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HNF68361.1"
                     /translation="MERRWSNYINQNFGTRILSKWLVFCFDIVITIFTYGVAYILRYN
                     FNIAIISFSTFVDNTFLTTLVFTASYLAFKTYDGIIRHSGEAEARRIVMSGMLATIVC
                     LALSIIGRETGKPFLTLPASIAIIHTSINIAFLLFSRYVVKVLFYQATRNNAAAIPVI
                     IYGAGRRGLSVMHTLRRDTNHNYQITAFLDDNPSKFQKTIEGVRIYNSEKLPDLLRRF
                     VVKELIISIAHPIPDKKMAVVDACLQHQVFIKSVPPVDDWINNKLSTRQLRKFNIEDL
                     LGREQIHLENDKLLSAIGGKTILITGAAGSIGSELVRQLIPFQPKQLILVDQAESDLY
                     DLYMELQFRLNEKKIVTENIIADITNRKRLEDIFATYHPAFVFHAAAYKHVPLMELNA
                     IEATHVNVIGSMYLTDLAIQYEVEQFIMISTDKAVNPTSVMGATKRAAEIYVQERSQH
                     SRKTTFITTRFGNVLGSNGSVVNYFNKQIENGGPLTITHPDVTRYFMTIPEACQLVLE
                     AAVMGKSSEIYMFDMGDPVKITDLATKMVLLSGYTPGKDIQFTFTGLRQGEKLHEELL
                     HSNENTLPTYHQKIRIALTAPYPAEHVQHYLQMLQDAIVNSDIQQSVRLLKLLIPEYK
                     SNNSEFERLDKQDTAYK"
     gene            complement(3838..4932)
                     /locus_tag="PLL28_03250"
     CDS             complement(3838..4932)
                     /locus_tag="PLL28_03250"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNF68362.1"
                     /translation="MSGEEQRFVNDAFDSNWIAPLGAHVDGFEQELSAFTGSNDCAAL
                     SSGTAALHLALIMLSVGKGDIVICPTLTFSASANPIIYQGATPVFVDCDATSWNMDPN
                     LCESAIKELIAKGKKPKAIILVHLYGMPSDMDAFIRISHQYDIPLIEDAAESLGATYR
                     NQHTGTFGEFGILSFNGNKVITTSGGGALLCKTKEQANYARFLATQARDNAPHYQHSV
                     IGYNYRLSNICAAIGRGQMTVLSERVQRRREIFHYYYNNLKDTEGISFSTEMEQMKSS
                     RWLTTILINPDQTGTDREQIRLLLGASGIESRPIWKPMHLQPVFNDATTYNQGVAENI
                     FQYGLCLPSGSSLTAAQQQEIIDLIRSAVK"
     gene            complement(4981..6015)
                     /locus_tag="PLL28_03255"
     CDS             complement(4981..6015)
                     /locus_tag="PLL28_03255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF68363.1"
                     /translation="MHITISPYNHISQITSLGLQPDDVYQIPSYCEMDALSIHAQPIL
                     ITATGAHGALALPLLLRQIPDAIAKGEKLFDAISPYGYAGILTNISDIGQLTEALNAC
                     RLEASKKNIVSIFIRMHPIHQKLIIPPTTSLNQVIHGNTVYINLENPVEYIKVSYSTG
                     LKRDLRKLMQLKIETKFNAWEYYDEFIEAYKETMALHQASNFYYFSRSYFESLRQPEF
                     NSILISSHTPDGTFISGALFFAHGNTVQYHLGGTKQSTRHLAGSKLIFDHAIDYFKLD
                     NRKAIHLGGGVGSAEDSLFRFKAGFSNERATFSTLRIICDEIKYQSLSADKISNMQNS
                     AKDFFPLYRL"
     gene            complement(5990..7135)
                     /locus_tag="PLL28_03260"
     CDS             complement(5990..7135)
                     /locus_tag="PLL28_03260"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF68364.1"
                     /translation="MYKHFIKRFLDIVFSLTAIAITLPFMVIIAVVCAITFKGQIFFI
                     QDRVGKNGAIFRLYKFITMRPQPAGKDLADTERLTAWGNFLRKSSLDELPQLFHILIG
                     DMSLIGPRPLLVRYIPYYTSAEWHRHDVKPGLTGLAQINGRNTLDWEARLNFDVVYAA
                     SYNFKMDLEIFIQTFIVVLKRSKYHIDPRTVVADFDIERKGNQIHISEGVILRPLYIS
                     DAGSLLEVKNNKEASVWLEKDPPVYTKEGLINWIRHHQSNPNNLILIIEDTINRKVIG
                     HAGLYDINPEDGTCIFGILIGLPEYWGKGIGRAATSAMIQIAFNTPGIQTIKLHVLQI
                     NERAISLYRSMGFTNEEVLVDQTIKNGEPQDVICMKMFNHAHNDISI"
     gene            complement(7141..8382)
                     /locus_tag="PLL28_03265"
     CDS             complement(7141..8382)
                     /locus_tag="PLL28_03265"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNF68365.1"
                     /translation="MHILLIHQYYQEKDDPGGMRWNAMTKLWAAEGHQITVIAGMTNY
                     ASGIKHPRYTNKYTYSEQFDTNIRVIRTHVSKSYNKSFRGRLNAYFSFVFSGIYGGLF
                     KARDKYDLILVSSPPLSVGIIALTLAFFKRRPFALEIRDLWPESAIDTGVLTNSTMIK
                     WSYRLEALLYRKALVTNVVTPAMQEVLINNKKVPANKVIYIPNAADFDMSDVLLLNFD
                     TAELRETLQLNGKLSLCYVGAHGVANHLEQLIIAAEKLRDLPVMFVFIGDGMQKQVLI
                     EMVTARNLKNVTFIGLITKSEALKYILACDIGLSVLKKADTFKTVYSNKTFDYMACKK
                     PVIMAIDGVSRKLIEDADAGIYVEPENTDEMIKAISTYLQHPELIKSHGENGYAYVKQ
                     HFDRKTLAQQYIRELTGRCSK"
     gene            complement(8522..10408)
                     /locus_tag="PLL28_03270"
     CDS             complement(8522..10408)
                     /locus_tag="PLL28_03270"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HNF68366.1"
                     /translation="MIRFIELYVDLFKGVGFKYWAYRFYHGLKKRTGILQYQFPIQAF
                     EPSDISKDFTIAPWGTFGNIHLDHTKIENNRETVTQRFTDVNNGHIQFFDGDVLKFTG
                     ENRWTTNAKTGKVYDNKVHWSNLNDFASEEDIKYIWEPARFNYLYAVLQYDQCFGTDN
                     SACIWQEICSFIDHAEMNAGPHYMSSQEIAIRIINWTIFLDIYKHSSSLTTEVYQKIT
                     GSIHTQAIHIFKYLDFSKNLVRNNHVLSEAAGLYIIGTFFPQFPDSEKFRKVGWSLFE
                     KEINYQIAGDGSYLQYSMNYHRMVIRLFSLMLRVGKVNGHHFQQETIQKGLTTLEFVN
                     AFIDEKSGTVANYGNNDGTSLFPFTDTPYNDYRTCIATFSIALGANNPYPQLIEDAQW
                     FGFDIESGSKTEIPKQDLYHFNEGGYYIFNEQYSKTFIRCGDHPFRPSQADNLHIDII
                     YNGMNIMRDAGTYQYNSTPEVTAFFMGTKGHNTVSIDGLDQMLKGPRFVWHFWTRKKY
                     AELTKSTDTITFKGEISAFRQTGRWINHRREVVKNMSSQTWVITDTILDKQKHIMTQF
                     WHPHPDFFSMFEIKAVDAKGEIIQPEITEALYSPTYGIAENCTQIAFHTGGNTITTVI
                     QLLQ"
     gene            complement(10405..10845)
                     /locus_tag="PLL28_03275"
     CDS             complement(10405..10845)
                     /locus_tag="PLL28_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68367.1"
                     /translation="MQPPVIIYRISRFFYDLKIKPISWLFDYSNRLFFKCWIPGSARI
                     GKNFTVGYWGIGVIIHSNTIIGDNCQVNQNTTIGKKITTNGVPKLGNNVYIGANCVIY
                     GAISIGDHAVIGPLSLVNTDIPANTFAAGIPAKPIKENSSSKDT"
     gene            complement(10820..11854)
                     /locus_tag="PLL28_03280"
     CDS             complement(10820..11854)
                     /locus_tag="PLL28_03280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNF68368.1"
                     /translation="MKKALIILPYANPHLGLFIKDITRIYDCDFYVDGPQSKYRNHFE
                     LHDVAIKSETTMKVLGNYFKGKYDVLISHGMFHAFSFFLHIGPRKPLIILSEPLKIPT
                     KAGLLYWLKRSIIRSLKRKHQLNIFILGDLSNSANFQSLAGQEIPCFPYGYFPDWQST
                     TNTKSAHADPVKFVYAGQFIERKNILLLLAGINLYCNKNGHVEFHFAGEGPFKSEIEA
                     NSNITFHGKLDAENVRKLLSASDVMILVSRSEGWGAIVNEAAACGCALMLSEAVEAKY
                     LFLQDGNTGVIVKESPQDIADAITELSHDRNKLQHMKNNVQELYSTIINSHFNKLTKL
                     LNNATSGNHI"
     gene            complement(11851..12975)
                     /locus_tag="PLL28_03285"
     CDS             complement(11851..12975)
                     /locus_tag="PLL28_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68369.1"
                     /translation="MKGNVTTYIMFYLFGMMHTMSYTTSLLLGFTLDMERGVYGLLLL
                     LLYYKYLDRRTIYLNIAVLYISIFTSIYTFDIKAHVQECIGFVLYYNVALIFWKVLGS
                     RQLTPTERLKHFYSFLSIPLLVYISPILLTPNNINLDFYSDPNLEKIGLKSRTVGWAS
                     ACSIPMVLQWTRKDGPRKFIIYLALAILIVLTIGSGSRSAIIGASLFVLALIYSFNIK
                     QRISYAILLIFFTAFILQNLDQLSLSRRAELHKMGVSDDTFRQNLVLAFSEHLVEDFP
                     SSLKPEGLGEPNIQHGLNKFFDTKGFGTHNSYISIILGLGFFSIIFLYRMFRGLKKLA
                     RLNYLKYFLPFLTISITEDCFGPGQLLYFFLIGSMVVLTE"
     gene            complement(12965..14062)
                     /locus_tag="PLL28_03290"
     CDS             complement(12965..14062)
                     /locus_tag="PLL28_03290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNF68370.1"
                     /translation="MTVVIFVTSVYELPNSGPATFAKILSESFHDSEIKFIIVTSDAI
                     NTADDLVVIRRPKSSVALYFQLWRKAKEIAAFYKDDYVIIHYNNSFPYAAFGTFGNRT
                     ILQMNDYYNIRLPYISFLHGSIRRSFAHIFRKLTERLSIHHSDVVLCNSRHTQNEAIG
                     YYKGQHQKFRLLYKSIDDADLGIKTIDEPSGEILYIGNDLKLKGFDILAEALKSTSRV
                     QKLHIAGPAANNPLLNQLLLHVPQHIAIVKHGALTHKELYALSPNLDMLVLPARYEAL
                     GVSVIECLAQGLPVLTSGVGGLMEILEEYPAEFYSASNLKPSILASNIDLILENYKYF
                     YTAVKERIPTIRNKFEKRKMTNYLKTLYLER"
     gene            complement(14115..14933)
                     /locus_tag="PLL28_03295"
     CDS             complement(14115..14933)
                     /locus_tag="PLL28_03295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNF68371.1"
                     /translation="MLINAIAFLFQIYVCKYIFRIKIVATLHNIKPHDAKNLHLHFFI
                     YKQFLKCCIKIRVFSNTTIAAASKYYDINTNKFFVIPEGSFVNYYPNNKSKAESRKTL
                     ELPDDAFIFLFLGNIRPYKGIQALLDIYKASEIKDSILIIAGNSPDKAYLEKIQKQTN
                     TSIIIKPGFITTENVQLFYNAANIVVLPFTEVENSGSAILAMGFEKAVIAPKKGVLSE
                     RLSEQPELLYNDGELGLTLKHAAVLSELELRKIGQRNAKHLGKYQWEDFCAFFI"
     gene            complement(15184..15726)
                     /locus_tag="PLL28_03300"
     CDS             complement(15184..15726)
                     /locus_tag="PLL28_03300"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNF68372.1"
                     /translation="MKTLNLILYSFYLYLINRFITNFPFQAIRRFFFKLLLKKIGTHN
                     SFLMGVQFRNPRNIIIGDNCIINKNVMLDGRNSKLLIGNNVEISQETNIWTLEHDIHD
                     DHFADKGGEVKIEDYVWIASRVTILPGVTIGKGAVVASGSVVTKDVPSMAIVAGVPAR
                     QIGVRESKLVYKKIHRPWFQ"
     gene            complement(15723..16985)
                     /locus_tag="PLL28_03305"
     CDS             complement(15723..16985)
                     /locus_tag="PLL28_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68373.1"
                     /translation="MLTFFDFRNDIRKLISSISKKEKVVVFVRKEHEHLVSSYQSIDF
                     TYRIIDEQQPSIINFLLTRCFLLFRKIPSSRNNYYLMEAFKAEGLPDKKQSRKAKKIL
                     KWQKLLPKFFSYDKYLSMLRPSKKTDLGGINSMLVLTEIYDDLLFARIIKSKLPTHVY
                     VYSWDHPCKHVRFSKKVNYLVWNKGISEDLQKLQQIPENQVSVIGSTQLGYIHEYLRQ
                     SAQEFSAKQNWIYYACGVGIRSLVHEEVSVIITLAKAMQATIPDHKLLVRPYPNLSNW
                     NLYDALADFDNITVDSSYRQTDLSVSEKDIFLKFSGIDAANALFHTGTTLGLEACFLN
                     TPSFIIDIANRKRSDVCLYNFVHQYQNNKYLIEKSPINVVYDEKALHHILQNTADPIY
                     MHLNKDISSAFEVLSFDDLAANFLNHLS"
     gene            complement(17003..18304)
                     /locus_tag="PLL28_03310"
     CDS             complement(17003..18304)
                     /locus_tag="PLL28_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68374.1"
                     /translation="MRFSVEFLEDKETIKFSRALSTSITLSVLAAICISLIFLGMGFY
                     GKQLFQLTGNTATICLPVFLLAAANSFFLVIGAVPQQLLQANNFFSLRNNWQYICQAL
                     NLIALIALWRCGINDVILYSIAIVFISIISLLIDITLISRKMILVGTKLQFKIERKRT
                     SVSQYSFRAFLHAIISFLAVQADKLIIGTVLNVGAVTTYSIITKPYYLLRGMTGTGYA
                     VFQPTLTALQFNSDKSTYLNYSSKIIRTTFIVVMSICCVAGICFEQGLTIWLKTSQYN
                     EMATWGIISLVSVCLTMLYTPFYRTMLYTSSLQTILNFSMLVVPINVVISILLTRAYG
                     FYGVIIGTLIQILLESGFVLFQARKLFNLKYTNIFTKPFNFRFLSILIIFIIGRFCIA
                     NITTSGLLISTEITLVLTLILISLNTIRKEGLFSQPINNGN"
     gene            complement(18514..18993)
                     /locus_tag="PLL28_03315"
     CDS             complement(18514..18993)
                     /locus_tag="PLL28_03315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HNF68375.1"
                     /translation="MKNKIKVFLTDVDGVLTDAGMYYTASGDEFKKFNTHDGMGMVML
                     QKAGIKTGMLTTEDTPIVKRRADKLKVDYLYQGVGFGGKLAAAQEICAKEGITLNEVA
                     YIGDDINCIELLQAVGLAACPANATPKVKAIAGIIHLQKSGGEGAVREFIDGYILQS"
     gene            complement(19005..19829)
                     /locus_tag="PLL28_03320"
     CDS             complement(19005..19829)
                     /locus_tag="PLL28_03320"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNF68376.1"
                     /translation="MKQTRIPLDNKVILLTGAFGLIGKTLSKAILESGAKLVLCDHNA
                     SKIEDMREHLLPFREGADYLICITDITSSSACAKCVDDAVGAFDHIDVLINNAAIDAK
                     FDAQSNDVLNATRFENFPIEQLDRSYEVNIKGLILITQTVVRQMLKQQNGNIINVAST
                     YSLVAPNQSLYDFGSEEIHYKPVDYVATKSMIPNLTRYLATFYAKDNIRCNAIAPHGI
                     FNHHSESFVVNFEKLSPMGRMAGRDEMTGPFLFLASDASSYMTGSVLTVDGGWTAW"
     gene            complement(19826..20788)
                     /locus_tag="PLL28_03325"
     CDS             complement(19826..20788)
                     /locus_tag="PLL28_03325"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNF68377.1"
                     /translation="MDNRTPILVIGAGSIGERHIGNLLSLGYENITVYRSRNLPLRTI
                     DTSKIKIITSLADIKTIPFEAAIICTPTSLHAEQTLFCLEQGMHVLVEKPLSHKTHQL
                     SEIAETCKRTGKLMQVAYMMAFHPHLQKMQKAIEEESFGKLLFIDTYWGSYLPDWHPW
                     EDYKQGYAARKDLGGGAALTLSHDLDMANRLANAPVAQFSKQYQYPKHLDINVEGIAD
                     FQIRYMNNVVAHVHMNYVDRVPRRTYRCVFEDGCMEMDFFAKTLTTVTATSTNKETLE
                     DFDRNDLFIAELNHFFDRIQSNTDHSAYSFDQILHSATIIEMCL"
     gene            complement(20781..21506)
                     /locus_tag="PLL28_03330"
     CDS             complement(20781..21506)
                     /locus_tag="PLL28_03330"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HNF68378.1"
                     /translation="MEVLAIIPARGGSKGLPGKNIRPLNGHPLLAYSILAAQQSELIT
                     RIIVNTDDLDIAAIAQQYGSEIPFMRPANLALDNTTDFEVFSHQLKWHLENEQYRPDI
                     IVQLRPTSPIRMPGWIDDGIRAVVERNADSLRIITEAPNTPYKMWTLGADKHAPMEPL
                     LSIENIAEPFNMPRQQLPKVYWQVGTLDVIATSTIEAGGMSGKKILPMIIDASLSVDI
                     DDLASFEKAEKIIAAQPCIKFHG"
     gene            complement(21513..22397)
                     /locus_tag="PLL28_03335"
     CDS             complement(21513..22397)
                     /locus_tag="PLL28_03335"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNF68379.1"
                     /translation="MFQFDYRMPKVIAEIGCNHMGDFDIAKELIRLAKECGADYAKFQ
                     KRNPKELLDNNQYNAPHPEPWQSYGDTYGAHREFLEFTVEQHAHLKSYAESIGIGYSA
                     SVWDITSASELIGLNPDFIKIPSACNTNFALLEILRDTYNGDIHISVGMTTSDETEKM
                     VSFFEQTNSAKNRLVLYSCTSGYPIQFSEICLLEINKLYQQFGERVKEIGFSGHHLGI
                     AADIAAYTLGARWIERHFTKDRTWKGTDHAASLEVPGLQKLVRDLHHMQQALQYKHED
                     ILPVEQAQRKKLKFKSGN"
     gene            complement(22463..22915)
                     /locus_tag="PLL28_03340"
     CDS             complement(22463..22915)
                     /locus_tag="PLL28_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68380.1"
                     /translation="MAKEMHESTNAVRLELNKLSKAGFLVSYGEGNTIQYKANDKHPL
                     FPELNSLVRKYLGIDKIVENIVKQIGEIHYALITGDYAEGKDTGNIDLMIVGEVDPER
                     LQYWVLKTETLIKRKVLTTVVNEQTFALYKENGILNSSIVLWSNGVAE"
     gene            complement(23129..24184)
                     /locus_tag="PLL28_03345"
     CDS             complement(23129..24184)
                     /locus_tag="PLL28_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HNF68381.1"
                     /translation="MDEKKFFQGRGSQINPFNPYHDINYAQEHIEGLDESWPAPGETQ
                     FFTEYPKRIVNFVDSPDSPMGISVNPYQGCEHGCVYCYARNTHQYWGFSAGLDFETRI
                     ITKPDAPKLLEKAFNDKNWVPAPVSFSGNTDCYQPVERKLRLTRGLLEVCQRYRNPAG
                     IITKNRLILRDLDILEELAQHDLIKVYISVTTLDESLRRAMEPRTSTAANRLDILKKL
                     SEKHIPVGIMIGPVIPGLNDHEIPALLAAAAEHGAFDAGFNMVRLNGAVGQIFSDWIH
                     KAFPDKAERVLNNIRAVHQGQLNDSRWKLRMRGDGHLAESIHKLFRLSRDKYMPERKM
                     FTYNTSLFRRPGQMRLF"
     gene            24262..25050
                     /locus_tag="PLL28_03350"
     CDS             24262..25050
                     /locus_tag="PLL28_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HNF68382.1"
                     /translation="MSNIASNLRHLRGERKLSQQELADMIGIGRTTIANIESNLANPG
                     HKTLLEIAKVFNVTIDELLHADLTKRILIQEKPAQHVAFRPVVVTVDELGQENIVLVD
                     TKAAAGYPTRYLEPEYYRELPAFKLPGSDYRNGTFRCFQVDGDSMQDTIQHGDFVVGR
                     FCDNHFKDVRDGYIHVVVTNDNVLVKRIISRAESEHKLYLISDNDAYPEIVVDIDDVR
                     EIWFVKSKISSYMPVKKNDLDKVIGDLTMQVHLLKSRIDQLEKN"
     gene            25295..25672
                     /locus_tag="PLL28_03355"
     CDS             25295..25672
                     /locus_tag="PLL28_03355"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HNF68383.1"
                     /translation="MIAPSNIITNRQRIAIGEELIWQQLKEKRVFGLTFARQYPFYLQ
                     QPDGERLVYSADFYCHDAGVAVRIVNCDAITAEYNEQMAWCKQQLQMRGILVIELPCD
                     AIADLKQVIAVIRRELKYKTIHA"
BASE COUNT         7549 a         5031 c         5041 g         8255 t
ORIGIN      
        1 acaatatttg tttcagcaat ttcagggctg ataagctgat tgttttgctt gaaatttttc
       61 aggagtgttt cctggttaaa caattgaatt tcaatggagt cgatctgatc ctttataaac
      121 ccaattactg ttttagcgct ttctgctttt cgatgcagac tcattttcac aatctcatcg
      181 gcgatgccat tgactacatc ctgggttttt tgcctgtttt tgtcgcggta tttaatgctt
      241 atttcgccgc catataaatt ttcaacatca atagaaacgt cttcttggat acgctcagac
      301 tcttttaaaa ggtcgttgat agtgaaaaag tattcgaact tgaattttga tttggcaaat
      361 gtattgaggg ttgtattgat attaaatgca ggggtgctat atgctttatt aaacgagaat
      421 tttttagaaa acttttcatt tttataagta taactcaatt tgtattctga ggtatctagt
      481 atttgaacat caattttatc gcctattatt gctgaatcta aaatggcaat atttaccgta
      541 aatggcgatt cgttatagat ttcggcagct ttaactcttt cacgaatata atatgacaca
      601 ccaataggta aatctgaaag tgctgtaatt ttataaccct tagaactaag aacagtcatt
      661 tcctcgttca tgcggtcaat attggaattg gctaccctga tgcctaagtc gatagtattg
      721 tctttggatt tatccttaaa ctgaatatcg gcatgagact cataaatagg tgtggcgtag
      781 cgattgtata agaatgcagc acaaacgcca attgcaaaaa caagcactat ccaccagcga
      841 ttaatccaac tgatggtaag gaataaacgc cagttaaatg actgatcatt ttgctgttca
      901 acgcttttat ggaattcttt attcggtgaa ttcattcctt attgattgac atttgcgaaa
      961 aatgcgtaaa taacagcaat gctggaaatg gtgcttaaaa taggaccaat ttcctgaaat
     1021 acagtactat tgatattcgg ttcgatatag ataatatcat tcggttgaat gtacatattg
     1081 cctaaagcaa tatcatcaat gctgcttaaa tctatttctt gtaattgcgc agcaccttga
     1141 atattgcgaa ttacttttat tttcgatgat cttccatttt caggaatacc accggccata
     1201 cctattgctt ccaataatgt aaggtctgag cgatttaatg ctaattgttt agcatcgctg
     1261 cctccgcgat aaacggtaat gtacttacct gtaataacca cattgacaaa agcatcagta
     1321 tatattttac catactcagt ggtcagcatt ttttctgctc ccgattcggt taaaccggag
     1381 attttcatcc acccgaggcg aggtaagttc gccattccgc tgccatcaat ctggtagctt
     1441 agtgattgca gcatggtttg cggttgataa taattcattt gattactaat ctgcgattcg
     1501 atcagattgt atccgccatt aggatataca aatacctgaa tttgatcacc cggttcgata
     1561 acaaactcgc gataaatggt atctaaaaac gacgattcaa caaaatcctt cccggtatcc
     1621 aacatgcgat tggggtacag cattccacat gatgaaaatg tagccagcag catacctgca
     1681 agtaataaat ggataatcgg cctttttgtc ggctgtttag agaaactggt catactgtat
     1741 gccctgaggc gaacttagcc tacaaatata gcatattcgg cgtattagtt taaagggcgc
     1801 tgattgccct gaaaccttat ttataagcgg tatcctgctt atctaagcgt tcgaattccg
     1861 aattgttact cttgtattcg ggtatcagca gttttaggag cctgactgac tgctggatat
     1921 cagaatttac aattgcatct tgcagcattt gtaagtaatg ttggacatgc tcggcagggt
     1981 agggggctgt gagtgcgatg cgtatttttt gatgataggt aggtaaggtg ttctcgtttg
     2041 aatgcaacaa ttcttcgtgt aatttttccc cctgtcgcaa gccggtaaaa gtaaattgga
     2101 tgtcctttcc gggggtatat ccgctcagta gcaccatttt ggtagccaga tcggtgattt
     2161 tgaccggatc gcccatatcg aacatgtata tctctgatga ttttcccatc actgcggctt
     2221 caagtactaa ttgacaggct tcaggaatgg tcatgaaata tctggttaca tccggatgcg
     2281 taatggtaag tgggccaccg ttttcaattt gtttgttgaa ataattgaca acagaaccat
     2341 tcgatccgag cacattgcca aatcgcgtag taataaaagt ggttttgcgc gaatgttgtg
     2401 atcgctcttg tacatatatc tcagcagctc gtttagttgc acccattacg cttgttggat
     2461 taactgcctt gtcggtagag atcatgataa attgctctac ttcgtattga atagcaaggt
     2521 cggttaaata catagagccg atcacattaa cgtgagtggc ctcaatagca tttaattcca
     2581 tcagaggtac atgtttatat gctgctgcat gaaaaacaaa tgcagggtga tatgtcgcga
     2641 aaatatcttc cagtcttttt cggttggtga tgtcggcaat aatattttct gtaactatct
     2701 ttttttcatt taaacgaaat tgcaactcca tatataaatc atagagatcg ctttcggctt
     2761 ggtcgacaag aatgagttgc ttgggttgaa atggtatcaa ctgccgcact aattcacttc
     2821 ctatagatcc ggcagcacct gttattaata ttgtctttcc accgatagca gaaagtaatt
     2881 tatcgttttc gaggtgtatt tgttctcttc ccaataaatc ttcaatattg aatttcctga
     2941 gttgacgggt gcttaattta ttattgatcc aatcgtcgac cggcggcact gatttaataa
     3001 atacctgatg ttgcaggcaa gcgtcaacta cagccatttt tttatcaggt attggatggg
     3061 cgatactaat tatcagctct tttacaacaa atcggcgcaa taagtcgggt aatttctcgg
     3121 agttataaat cctaacgcct tcaatcgttt tttgaaattt cgaagggttg tcgtcgagaa
     3181 atgctgttat ttgatagttg tgattggtat ctcttctcag tgtgtgcatt acgctcaagc
     3241 ctcgtcttcc ggcgccataa ataattacag gaatggcggc agcgttattg cgggtggctt
     3301 gataaaaaag cacttttaca acatagcgtg aaaataataa aaaggcgata ttgatagacg
     3361 tatggataat ggcaatagat gcgggcaacg taagaaacgg ttttcctgtt tctctgccga
     3421 tgatggatag cgccaaacaa acaatagtag ccaacatgcc tgacatcact attcttctgg
     3481 cttcagcttc gcctgaatgt cggataatgc catcgtaggt cttaaatgcc agataacttg
     3541 cagtaaatac taaggtagta agaaaggtgt tatcaacgaa agtagaaaat gaaataatcg
     3601 ctatgttgaa attatatcga agaatatatg caacaccata tgtaaatatg gtaattacta
     3661 tatcgaagca aaataccaac cacttggaca atattcttgt tccaaaattt tgattgatat
     3721 aattactcca tctgcgctcc atttgcctga tagattttat tccaattagc ggaaattaag
     3781 ataatgctta cgcacacctc tgtcacaaat ctacgatatt ttaacacttt tatgggtcta
     3841 tttcacagca gagcgaatga gatcaatgat ctcctgctgt tgggctgcag taagcgaaga
     3901 gcctgaaggc aggcaaagcc catattgaaa gatgttttcg gcgacacctt gattgtaggt
     3961 agttgcatca ttaaaaaccg gttgcaggtg catgggtttc caaattggtc gcgattctat
     4021 tcctgatgct ccaagtagca atctgatttg ctccctatca gtccctgttt gatcagggtt
     4081 aatcaaaatt gtggtgagcc atctggaaga cttcatttgt tccatttcag tagaaaaaga
     4141 aatgccttca gtatccttca gattgttata gtaataatga aatatttcgc gacgacgttg
     4201 aacacgttct gatagcacag tcatttgtcc tctgccgatg gctgcgcaaa tattacttaa
     4261 tcgataatta tatcctatca ctgaatgctg ataatgagga gcgttgtctc tcgcttgtgt
     4321 ggcaagaaat cgcgcatagt tggcttgctc ttttgttttg cataacaaag cacctcctcc
     4381 tgaagtggtg attactttat taccattaaa tgagagaatg ccaaattcgc caaatgttcc
     4441 ggtgtgttga ttgcgatatg ttgctcctag tgattctgca gcgtcttcaa tcaatggaat
     4501 atcatattga tgtgagatgc ggatgaatgc atccatgtcg gaaggcatgc catacaaatg
     4561 aactaaaatt atagcttttg gttttttccc tttggcgatt aattctttga tggcagactc
     4621 gcataaattt ggatccatgt tccaggaagt cgcatcacaa tcaacaaaaa cgggcgttgc
     4681 accctggtaa ataattggat ttgcagaggc tgagaaagtg agtgtggggc aaataacgat
     4741 atcacctttt ccaacactaa gcatgattaa tgcaagatgc aaggctgcag tgccggaact
     4801 taatgcagcg caatcattgc tgcctgtaaa cgcagataat tcttgttcaa aaccatcaac
     4861 atgcgcgcct agtggagcaa tccaattgct gtcaaatgcg tcgttaacaa acctttgttc
     4921 ctcacccgac atgtgcggtg gggatagata gattctgatt ttattgacag cgctcattcg
     4981 ttacagtcgg tacaaaggaa aaaaatcttt tgcactgttt tgcatattgg aaattttatc
     5041 agcagaaaga gattgatatt ttatttcatc gcaaataatt ctaagggtgc tgaaagttgc
     5101 tctttcattg gagaagccgg ctttaaacct aaataaacta tcttcagcgg atcctacgcc
     5161 accgcctaaa tgtatggctt tccggttgtc tagtttaaaa tagtctatcg cgtggtcaaa
     5221 tatcaatttt gaacctgcaa gatgtcttgt agattgtttt gttcctccca aatgatattg
     5281 aaccgtattg ccatgtgcaa aaaataatgc ccctgaaata aatgtgccgt caggcgtatg
     5341 cgatgaaatt aatatactat tgaattcagg ttgtctaagt gactcaaagt atgatcttga
     5401 aaaataataa aaatttgatg cctgatgaag tgccattgtt tctttgtaag cctcaatgaa
     5461 ctcatcgtaa tattcccatg cattaaattt tgtctcgatc ttcaactgca ttaatttgcg
     5521 caaatcacgc tttagtccgg tgctatatga tacctttata tactcaaccg gattttctaa
     5581 attgatatag acagtgttgc catgtattac ctgatttaag cttgtagttg gcggaataat
     5641 caacttttgg tgaatgggat gcattcgaat aaaaatgctg actatatttt ttttagaggc
     5701 ttctaatctg caggcattta atgcctcggt aagctgaccg atatcagaaa tattagttag
     5761 aattcccgca taaccgtaag ggctgatggc atcaaaaagt ttttcaccct tggcgattgc
     5821 atccggaatt tgtcttaata ataagggcaa tgccaaagca ccatgagcgc ctgtggcagt
     5881 gattaatatt ggctgagcat gaatgcttaa tgcatccatc tcacaatagg atgggatttg
     5941 gtaaacatca tcaggttgca agcctaatga ggtgatctga ctgatatgat tatatggaga
     6001 tatcgttatg tgcatggttg aacattttca tacagatcac atcctgaggt tctccatttt
     6061 taatggtttg atcaactaat acctcctcat ttgtaaagcc cattgagcga tataacgaaa
     6121 ttgcccgttc attaatttgc aatacatgga gctttatagt ttgaattccg ggcgtattaa
     6181 aggcgatttg aatcatagct gaagtggctg cgcggccaat tcctttgccc cagtattccg
     6241 gtaacccgat caggattccg aatatacagg tgccatcttc gggatttatg tcatacagac
     6301 ctgcatgtcc aatcaccttg cgattgattg tatcttcaat aatcagtatg agattatttg
     6361 gatttgattg atggtgtcga atccaattaa taagaccttc ctttgtataa accggagggt
     6421 ctttttcaag ccataccgaa gcttctttat tgttctttac ctcaagtaat gagcctgcat
     6481 ctgaaatata tagtggtctt aaaataacac cttctgaaat gtgaatttga tttcctttgc
     6541 gttctatgtc aaaatcagcg actacggttc gtggatctat atgatattta cttcgcttga
     6601 gtacaacgat aaatgtttga ataaaaattt caaggtccat tttgaaattg tatgatgcag
     6661 catatacaac atcgaaattt aatcgcgctt cccagtcgag cgtatttctg ccatttattt
     6721 gtgccaatcc ggtaagtccg ggcttaacat cgtgacgatg ccattctgcc gaggtgtaat
     6781 atggtatgta tctcactaat aatggtcgcg gaccaatcaa actcatatct cctatcaaga
     6841 tgtggaatag ttggggtaat tcatccaggc tcgactttcg aagaaaattt ccccaggcgg
     6901 tcaacctttc tgtgtctgca agatctttac cggcaggctg cggacgcatg gtgatgaatt
     6961 tatacaatct gaaaatggca ccgtttttac ctacccgatc ttgtataaag aatatttgtc
     7021 ctttgaatgt gatagcacaa acaactgcta tgatgaccat gaaaggtaaa gtgatggcaa
     7081 tcgccgtgag cgaaaaaact atatcaagga atcttttaat aaaatgttta tacacgattg
     7141 ctatttagaa cagcgccctg taagttcacg aatgtattgt tgggccagtg tttttctatc
     7201 gaaatgttgt ttaacatatg catagccatt ttcgccatga cttttaatta gttcaggatg
     7261 ttgcagatac gttgaaatgg ctttaatcat ttcatcagta ttttcaggtt ctacatagat
     7321 accggcatct gcatcttcaa ttaatttgcg ggatacgcca tcaatcgcca tgattaccgg
     7381 cttcttacat gccatgtaat caaaggtttt attgctataa actgttttaa aagtatctgc
     7441 tttcttcaat acagacaaac cgatatcaca agccagtata tattttaaag cttccgactt
     7501 tgtgatcaac ccgataaagg tcacattctt gaggttccta gcagtaacca tttcaatgag
     7561 tacctgtttt tgcatgccat cgccgataaa aacgaacatt accgggaggt ctctcaattt
     7621 ttctgcggct attatcaatt gctccaaatg atttgcaacg ccatgtgcac ctacatagca
     7681 aagcgataat ttgccattga gttgcagtgt ttcgcgaagc tcagcggtat caaagtttaa
     7741 aagcagtaca tcgctcatgt caaaatccgc ggcattcggg atataaatga ctttatttgc
     7801 gggaactttt ttgttgttga taaggacttc ctgcattgcc ggagttacaa catttgttac
     7861 aagtgctttt ctatataata atgcctccag gcgatatgac catttgatca tggtgctgtt
     7921 cgtgagcact ccggtgtcaa tggcagattc gggccataaa tctcgtatct ccaatgcaaa
     7981 tggtctgcgt ttgaagaagg caagtgtcaa agcaataata cccaccgaaa gtggcggcga
     8041 actcactaaa atgagatcat atttatctct tgctttgaat agacctccat aaattccgga
     8101 aaaaacaaat gaaaaatagg catttaatct gccacgaaaa cttttattgt atgattttga
     8161 tacatgtgtt ctgatgacgc gaatattagt atcaaattgt tcggaatagg tgtatttgtt
     8221 agtatatctc ggatgtttaa tgccactcgc ataattagtc ataccggcaa tcactgtaat
     8281 ctgatgacct tcagccgccc ataacttagt cattgcattc caacgcatgc cacccgggtc
     8341 atctttttcc tgataatatt ggtgaatgag taaaatatgc attgggttga tcgggctaca
     8401 gcctttgtgg tggctggggg ataaaggtaa tcaccaatta atatatttca aaaatgtgaa
     8461 attgatatcg gcgacaataa tttgaacgga tgttgcttta attccttatt gctcacttat
     8521 actattgcaa tagctgaatt actgtagtaa ttgtgtttcc acctgtatga aaggcaattt
     8581 gagtgcaatt ttccgcaatc ccgtatgtgg gtgaatacaa tgcttcggta atttcaggtt
     8641 gaattatttc cccttttgca tctacggctt taatttcaaa catgctgaaa aaatccggat
     8701 gtggatgcca aaattgtgtc atgatatgtt tttgtttatc gaggatggta tcggtaatga
     8761 cccatgtttg gctactcatg ttttttacca cttctcttct gtgattaatc catcttccgg
     8821 tttgccgaaa ggcggaaatc tctcctttga aagtgatggt atctgttgat ttggtaagtt
     8881 cggcatactt ttttcttgtc caaaaatgcc agacaaaacg aggacccttc agcatttggt
     8941 ctaatccgtc aatggaaaca gtattgtgtc cttttgttcc cataaaaaac gcagtgactt
     9001 cgggtgtgct attatattga taagttccgg catcacgcat gatattcatt ccattgtaaa
     9061 ttatgtcaat gtgtaaattg tcggcttgcg atggcctgaa tgggtggtct ccacatcgaa
     9121 tgaaggtctt agaatactgt tcattaaaaa tgtaataacc accttcgtta aaatggtata
     9181 aatcttgttt cggtatttct gttttgctac cggattcaat gtcgaatcca aaccattgtg
     9241 catcttcaat aagttgagga taagggttat ttgcacccaa ggcaatgctg aatgttgcta
     9301 tgcatgtacg ataatcgttg taaggcgtat cggtaaaagg aaataaagat gttccatcat
     9361 tgtttccata atttgcaaca gtccctgatt tttcatcaat aaaggcattt acaaattcca
     9421 gcgtcgtcaa tcctttctga attgtttctt gttgaaaatg gtgaccgtta acttttccta
     9481 cgcgcaacat cagtgaaaat aaccgtatca ccatgcgatg gtaattcatt gaatattgaa
     9541 gatagctgcc atcgccggca atttggtaat taatttcttt ttcaaagaga ctccagccaa
     9601 cttttcggaa tttttctgaa tcagggaatt gtgggaaaaa agtgcctatg atgtataaac
     9661 ctgcagcttc tgaaagtaca tggttgtttc ggacgaggtt tttggaaaaa tcgaggtatt
     9721 tgaagatatg tatagcctgc gtatgaatag acccggtgat cttttggtag acttctgttg
     9781 ttaggctgct gctatgttta tatatatcta aaaatattgt ccaattaata atgcgtatcg
     9841 ctatctcctg gcttgacatg taatgtggtc cggcattcat ttccgcgtga tcgataaacg
     9901 agcagatttc ctgccatata catgcactgt tatcagtgcc aaagcattga tcatattgta
     9961 atactgcata cagataatta aatcttgccg gctcccagat gtatttaata tcttcctctg
    10021 aggcaaaatc attcaaattg gaccaatgta ccttgttgtc gtataccttg cctgtttttg
    10081 cattggttgt ccatcggttt tctccggtaa atttcaaaac atcaccgtca aaaaattgaa
    10141 tatgcccatt gttgacatca gtaaatcgtt gagttactgt ttctctatta ttttcaatct
    10201 ttgtatggtc caaatgtata ttgccaaaag ttccccaagg tgcgattgtg aaatccttgg
    10261 atatgtcgga tggttcaaaa gcttggatag ggaattgata ttgcagtata ccggttcgtt
    10321 tctttaggcc atgatagaat ctgtaagccc agtatttgaa gccaacgcct ttgaataaat
    10381 caacgtatag ttcaatgaag cgaatcatgt atcctttgac gatgaatttt ctttaatggg
    10441 ttttgccgga atacctgctg caaatgtgtt cgccggaatg tctgtattta ccaggctaag
    10501 tggtccaata actgcatgat caccaattga aattgcgcca tagatcacac aattagcacc
    10561 gatgtataca ttgtttccta atttcggcac accatttgtg gttattttct tgccgatggt
    10621 tgtattttga tttacctggc aattgtcgcc aatgatggta ttgctgtgga tgattacccc
    10681 tattccccaa tagccaaccg taaaattttt acctatccta gccgaacccg gaatccagca
    10741 tttaaaaaat aatcgattgc tgtaatcaaa caaccatgat atcggtttta tttttagatc
    10801 gtagaaaaac cttgaaattc tatatatgat taccggaggt tgcattattt agtagtttgg
    10861 tgagcttgtt gaaatgggaa tttattattg ttgaatacaa ttcttgaaca ttatttttca
    10921 tatgttgaag cttgtttcta tcatgtgaaa gctcagtaat ggcatctgca atatcctgag
    10981 gcgattcttt tactattaca ccggtattac catcctgtaa gaaaagatat ttagcctcaa
    11041 cagcttcgga aagcattagc gcgcaaccgc aggcagcggc ttcattaaca atggctcccc
    11101 atccttccga ccggctcact aagatcatca cgtctgatgc actaagtaat ttacgcacgt
    11161 tctcggcgtc aagtttaccg tggaaagtaa tatttgaatt tgcctcaatt tctgacttaa
    11221 aaggaccctc tccggcaaaa tgaaattcca catggccatt tttgttgcaa tataaattaa
    11281 tccctgcaag gagaagtaat atgttttttc gttcaataaa ttgtccggca tatacaaatt
    11341 ttaccggatc agcatgagca gattttgtat ttgttgttga ttgccaatcc ggaaaatagc
    11401 catagggaaa acaaggaatt tcctgccctg ctaaagattg gaaatttgcc gaatttgata
    11461 gatcccccag aataaaaatg tttaattgat gtttcctttt tagtgatcta atgatgcttc
    11521 gcttgagcca atataacaga ccggccttag ttggtatttt caatggctca gataagataa
    11581 tcagcggttt tcgaggtcca atgtgaagaa agaagctgaa tgcatgaaac atgccgtgag
    11641 aaatcaatac atcgtatttg cctttgaaat aatttccgag gaccttcatt gtggtttcgc
    11701 tttttattgc gacatcgtgg agctcaaaat gattgcggta tttagattgg ggtccatcga
    11761 cgtaaaaatc gcaatcgtaa atgcgagtga tatccttaat aaataagccc aaatgtgggt
    11821 tggcgtaggg caatataata agggcttttt tcattcagtt agcacaacca tgcttcctat
    11881 cagaaagaaa tataacaatt gccccggccc aaagcaatct tcagttattg aaatagtgag
    11941 gaaaggaagg aaatatttaa gataatttaa tcgtgcgagt tttttgaggc ctctaaacat
    12001 tctataaagg aagattatgg aaaagaatcc cagaccaagt atgatggaga tgtatgaatt
    12061 atgtgttcca aatccttttg tgtcgaaaaa tttatttaat ccgtgctgaa tatttggttc
    12121 gccaagtcct tccggcttaa gtgatgaagg gaagtcttct acaaggtgtt cggaaaacgc
    12181 taatacaagg ttctgcctga aggtgtcgtc tgataccccc attttatgca gctctgcgcg
    12241 ccgtgataag cttaattggt cgagattttg aagaataaat gcagtaaaaa aaatcaataa
    12301 tatggcgtaa gaaattcgtt gcttaatgtt aaaactatag atcaaggcta atacaaacaa
    12361 tgaggcgccg ataatagcag atctagagcc ggagccgatg gtaagcacta ttaaaatggc
    12421 caatgccaaa taaataataa atttccttgg gccatctttt cttgtccatt gtaaaaccat
    12481 gggaattgaa catgcggatg cccaaccaac tgtgcgcgat tttaaaccaa ttttctcaag
    12541 gttaggatct gaatagaaat caagattaat attattaggg gtcaataata tcggacttat
    12601 ataaaccagt aatggtatag acagaaagct gtaaaagtgt tttagccttt ctgttggtgt
    12661 caattgtcga ctacccaaca ctttccaaaa aatcagtgca acattgtaat atagtacaaa
    12721 gcctatacat tcttgcacgt gagctttaat atcaaaggtg tatattgatg taaatattga
    12781 gatatataga acggcaatat tcagatagat tgttctacga tctagatatt tataatataa
    12841 cagcaataac aagagaccgt aaactcctct ttccatatca agtgtgaacc caagaagtag
    12901 tgaggttgtg tatgacatgg tatgcatcat gccaaacagg taaaacatta tatatgttgt
    12961 tacattacct ttcaagatat aatgttttta agtaattagt catttttctt ttctcaaatt
    13021 tattccgaat ggtaggaatt cgctctttta ctgcggtgta aaagtattta tagttttcta
    13081 atataaggtc tatgtttgaa gccaatattg atggctttag gtttgatgca gaatagaatt
    13141 cagcagggta ttcttccagt atttccatta agccgcccac ccctgatgtt aatacaggga
    13201 gtccttgtgc taaacattca ataacagaaa caccgagtgc ttcatatcgg gcaggcagca
    13261 ccagcatatc cagatttgga cttaatgcgt ataattcttt atgcgttaat gcgccatgtt
    13321 ttactattgc aatgtgttga ggcacatgaa gtaataattg gtttaataaa ggattgttag
    13381 cggcgggacc tgcaatatgc aatttttgca ccctggatgt tgattttaat gcttctgcca
    13441 atatgtcaaa tccctttaac ttaagatcgt tgccaatata taaaatttca cctgatggtt
    13501 catcaattgt tttaatacct aagtcggcat catctatgct tttataaagc agtcgaaatt
    13561 tctgatgttg acctttgtaa tagccaatgg cttcgttttg agtgtgtcga ctattacaca
    13621 atactacatc agagtgatga attgacaatc gttcagttag tttacgaaag atgtgtgcaa
    13681 atgacctcct aatgctacca tgcaaaaatg aaatatatgg caagcgaatg ttgtaataat
    13741 cgttcatttg cagaattgtt ctgttcccga aggtgccaaa tgccgcgtaa ggaaatgaat
    13801 tattataatg gataattaca tagtcatctt tataaaaagc tgcaatttct tttgctttcc
    13861 gccataattg aaaatatagt gcaactgatg attttgggcg tcgaattaca accaagtcat
    13921 cagcagtatt tattgcatca gaggtgacaa taataaactt gatttcagag tcgtgaaaag
    13981 attcgcttag tatcttcgca aaagtggcag ggccggaatt gggcaattca tatacactag
    14041 taacaaatat aaccactgtc attcgctctt ccactatatt gattattaat tcacttttat
    14101 agaattatgt cctgttatat aaagaatgcg caaaagtcct cccattggta tttgcctaga
    14161 tgttttgcgt tcctctggcc aatcttcctt aactccaact cggaaaggac tgcagcatgc
    14221 tttagtgtta gacctaattc gccatcatta tatagtaatt caggctgctc gctaagtcgt
    14281 tctgataata cacctttctt gggcgcaatt acagcctttt caaaacccat tgctaatatg
    14341 gcagaccctg aattttccac ctcagtaaac ggtaaaacaa caatgttggc tgcattataa
    14401 aataattgca cattctctgt tgttataaag ccgggcttaa tgatgatact ggtatttgtc
    14461 tgtttttgaa tcttttccag ataggcttta tcagggctgt tgccggcaat gattaaaata
    14521 ctgtccttta tttcagatgc tttataaata tctagtaatg cttgtattcc tttgtatggt
    14581 cttatatttc ccaaaaatag aaaaataaat gcatcatccg gtaattctaa tgttttcctg
    14641 gattcagctt tggacttatt atttggataa tagtttacaa agctgccttc aggaataaca
    14701 aaaaatttat ttgtatttat atcgtaatat ttagatgctg cagcgattgt ggtattgctg
    14761 aagaccctta tttttatgca gcacttcaag aattgtttat atataaaaaa atgcaaatgt
    14821 aaatttttgg catcatgtgg ctttatgtta tgtaatgtgg caacaatctt tattctaaat
    14881 atgtatttac agacataaat ttgaaaaaga aacgcaattg catttatcag catcaatact
    14941 ttattcctgc gtgcatataa gctgtctatc cagtcgaaat gtatataatc gggaaatgag
    15001 ctgattgcag ttaagaagat gccaattagt ctatttttgt taccggaata ggcgtgtaat
    15061 gtttcattgg tgtttagtcc ttggatcatg agatattgaa atggatttcc aattccggca
    15121 acaggtaaac aggctatttt aacctttcga gtcatctgct ttttcgttta actgaagata
    15181 gttttattga aaccaagggc gatggatttt tttataaaca agtttacttt ccctcacgcc
    15241 gatttgacgg gcaggaacac cggcaactat tgccattgaa gggacgtcct ttgttactac
    15301 tgatcctgaa gctactactg cgccttttcc aattgtaacg cccggaagga tggtaactct
    15361 gctcgcaatc cagacataat cttcaatttt cacctcaccg cctttgtcag caaaatggtc
    15421 atcatgaatg tcgtgctcaa gtgtccaaat atttgtctct tgtgaaattt cgacattgtt
    15481 gccgatcaac aatttactgt tcctgccatc gagcatcacg tttttattga ttatgcaatt
    15541 gtcgccgata ataatattcc tgggattcct gaattgaacc cccatcagaa aactattgtg
    15601 agtgccaatt ttctttaata ggagcttgaa aaagaatcgc cttatagcct gaaagggaaa
    15661 attggtaatg aatcgattta ttaaatataa ataaaaagaa tatagtatga ggtttaaagt
    15721 tttcatgata agtggttcag gaaatttgca gctaaatcgt cgaaggagag aacctcgaat
    15781 gctgatgaaa tatctttatt taagtgcata tatatgggat cagccgtatt ttgtagaatg
    15841 tgatgaagcg ccttttcgtc gtaaacaaca tttataggag atttttcaat cagatattta
    15901 ttgttttgat attgatggac aaagttgtat aaacatacat cagacctttt tcgatttgca
    15961 atatctataa tgaagcttgg cgtgttcaaa aagcaggcct ccagtcccaa tgtggttcca
    16021 gtgtgaaata atgcattcgc tgcatcaatg cctgaaaatt taaggaaaat atccttttct
    16081 gaaacggaca aatcggtttg tctataacta ctatctactg tgatattatc gaagtcagct
    16141 aaagcatcat acaaattcca gttacttaaa tttggatacg gccttaccaa tagtttgtga
    16201 tccgggattg ttgcctgcat ggcttttgcg agagtaatga ttaccgatac ctcctcatgg
    16261 accaatgagc gaattccaac accacaagca taatatatcc aattttgctt tgcagaaaat
    16321 tcttgtgccg actgcctcaa atattcatgt atatatccaa gttgtgtaga acctattaca
    16381 gatacttggt tttccggtat ttgttgcaat ttttgcaaat cctctgaaat tcccttattc
    16441 caaactaaat agtttacctt tttactgaat cttacatgtt tacaagggtg atcccatgaa
    16501 tacacataga catgtgtagg taatttactt ttaatgattc tcgcgaatag cagatcgtcg
    16561 tatatttctg ttaaaaccag catggaattg attccgccca aatctgtttt tttcgacggc
    16621 ctgagcatgc ttaggtattt atcataggaa aaaaacttgg gaagcagttt ttgccatttc
    16681 aaaatctttt tagcctttct gctttgtttt ttatccggca acccctcagc cttgaaagct
    16741 tccataaggt aataattgtt cctgctggac ggaatttttc tgaataataa aaaacaacgc
    16801 gtaagcagga aattgataat ggatggttgt tgctcgtcaa tgatgcgata ggtgaagtct
    16861 attgattgat aactggaaac taaatgctca tgctccttgc ggacaaagac tacaaccttt
    16921 tcttttttgg aaattgatga aatgagtttg cgtatatcat ttctgaaatc aaaaaacgtt
    16981 agcatgacac cgattgccat atctagttgc cgttattaat gggttggcta aaaagaccct
    17041 cttttcttat agtattgagg gagattagta tgagggtcaa aacaagggtt atctcagttg
    17101 aaatgagtaa gccggatgtt gtaatgttag caatgcaaaa acgacctatg ataaaaatta
    17161 taagtattga caaaaagcga aagttgaagg gcttggtgaa aatgttggta tactttaaat
    17221 taaatagttt ccgtgcctga aatagcacaa atccgctttc taataaaatt tgaatcaacg
    17281 tgccaataat cacaccgtag aaaccatagg cgcgtgtgag caaaatactg attacaacat
    17341 ttattggcac aactaacatg gaaaaattta gaatcgtttg gagagatgag gtatacaaca
    17401 ttgttcgata aaacggcgta tacaacattg ttaaacagac tgaaactaaa cttattatcc
    17461 cccaagttgc catttcatta tactgtgatg ttttaagcca gatggttagc ccttgctcaa
    17521 aacaaatgcc ggcaacacaa caaattgaca tcactactat gaatgttgtt cttattattt
    17581 tactgctata gttgaggtat gtcgatttgt ctgaattaaa ttgtaaagca gttaaggtgg
    17641 gttgaaacac ggcataccct gtgccggtca taccccgtaa tagataataa ggttttgtaa
    17701 taattgaata agttgtaact gcaccaacat ttaaaactgt tcctataatt aatttgtcgg
    17761 cctgaacagc aaggaagctt attatcgcat ggaggaacgc cctgaatgaa tattgcgaga
    17821 ctgatgtgcg ctttctttca atcttaaatt gaagttttgt gcccaccaaa atcatcttcc
    17881 ttgaaattaa tgtgatatct ataagcaggg atatgatgga tataaataca atagctatgc
    17941 tatataagat aacgtcgttt attccacatc tccaaagtgc tatcagtgca atcaggttta
    18001 atgcctgaca gatgtattgc caattgttcc tcaagctgaa aaagttattc gcttgtaata
    18061 attgttgcgg aacagctcca atcaccagga agaaactatt tgctgcggct agtaagaaaa
    18121 cgggtaagca tattgtagcc gtattgcctg taagttgaaa cagctgtttg ccgtaaaatc
    18181 ccatccctag aaaaatgagc gaaatgcaaa tagctgctaa taccgatagc gtaattgaag
    18241 tgctcagcgc gcgactaaat tttattgttt ctttatcttc aagaaattct actgaaaaac
    18301 gcatcgctga tttagatgcc ccaaaatcaa atgcagtaaa cgaagaagaa agcccgaacg
    18361 ccaggaggaa taggccatac gtgtcggctc caaaagcttt gaggcaaatt ggaagcgtaa
    18421 gtatgccaat tcctagtttc gctacataca ggaatagatt acttgataga tttttaaaaa
    18481 cattagacat gccggtaaat agctatcagt ggatcaactt tgtagaatat acccatcaat
    18541 aaactccctc accgccccct ctccaccgct tttttgtaaa tgtatgattc cggcaatggc
    18601 tttcactttc ggagtggcat tggccgggca tgcagctaag cccactgctt gcaatagttc
    18661 aatgcaatta atatcgtctc cgatataagc aacttcattt agtgtaattc cctccttggc
    18721 gcagatttct tgtgctgcag ctagctttcc tccgaatcca acaccttgat ataaataatc
    18781 tactttgagt ttatcggcac gtcgttttac gatgggcgtg tcttcagttg tcagcattcc
    18841 tgttttaatg cctgcttttt gtaacatcac catgcccatt ccatcatgcg tattgaattt
    18901 tttaaattca tctcctgagg cagtataata catgcccgca tctgtcaata cgccatcaac
    18961 atcagtcaga aatactttta ttttgttttt catatcgatt tattttacca ggcagtccaa
    19021 ccaccatcta ctgttaatac acttccggtc atatagcttg acgcatcgct ggcaagaaat
    19081 aaaaatggtc ctgtcatttc atctcttcct gccatccttc ccattggcga aagcttttca
    19141 aagttcacaa caaatgactc gctgtggtga ttgaaaatgc catgcggcgc tattgcatta
    19201 catcggatgt tatccttcgc ataaaaagtt gcgagatacc ttgtcagatt gggaatcatt
    19261 gatttggttg ccacataatc taccggttta taatggattt cctctgaacc gaaatcatac
    19321 aaactttgat ttggtgcaac taatgaatat gtgctcgcta cattaataat attgccattt
    19381 tgttgtttca gcatttgcct tacaacagtt tgagtgatga gaattaatcc cttgatgttt
    19441 acctcgtaag aacggtctaa ttgttcaata gggaagtttt caaagcgcgt tgcatttaat
    19501 acatcattgg attgcgcatc gaatttggca tcgatggcgg cattattaat caacacatca
    19561 atatggtcaa aggcgccaac ggcatcatct acgcatttgg cacaggcact tgatgaagta
    19621 atatctgtga tacagattaa ataatctgcg ccttcgcgaa agggtagaag atgttcccgc
    19681 atatcttcaa tcttggaagc attgtgatca cacaaaacca attttgctcc tgattcaagt
    19741 atagccttgg ataaggtttt tccaatgagt ccaaatgcac ctgttaaaag aatgaccttg
    19801 ttgtctaatg gaattcttgt ttgtttcata ggcacatttc aattattgta gctgaatgta
    19861 aaatttggtc gaatgagtaa gcgctgtgat ctgtgttaga ctgaatgcga tcaaagaaat
    19921 ggtttaattc ggcaatgaat aaatcatttc tatcaaagtc ttctaaagtt tctttattgg
    19981 ttgatgtggc tgttactgtt gtaagtgttt tagcaaagaa atccatttcc atacaaccat
    20041 cttcaaacac gcagcggtat gtgcgacgag gtactcgatc gacataattc atatgcacat
    20101 gtgcaaccac attattcata tatctgattt gaaaatctgc aatgccctca acatttatgt
    20161 ccagatgctt tggatattgg tactgctttg aaaattgtgc aaccggagca ttggcaagtc
    20221 ggttggccat gtctaaatca tgacttagtg tcaaagctgc tccgccacct agatcttttc
    20281 gcgcagcata accttgttta tagtcttccc aaggatgcca gtcgggtaaa taacttcccc
    20341 aataagtgtc tataaaaagt aattttccaa atgattcctc ttcaattgct ttttgcattt
    20401 tttgcaaatg cggatgaaaa gccatcatat aggctacttg catcaattta cccgtgcgtt
    20461 tacatgtttc cgcaatttca gaaagttgat gcgttttgtg cgacaaaggt ttttccacca
    20521 acacatgcat gccctgttcc agacaaaata atgtttgctc ggcatgtaaa gatgtaggag
    20581 tgcaaataat cgctgcttca aatgggatcg tcttaatgtc tgctagcgat gtaataattt
    20641 tgattttgga tgtatcaatt gttcgcaagg gtaaattgcg acttctatac accgtaatat
    20701 tctcatagcc aagcgaaagt aaatttccga tatgtcgctc tccaatagag cctgcaccta
    20761 ttactaatat tggtgttcga ttatccatga aatttgatac aaggttgcgc tgcaataatt
    20821 ttttctgctt tctcgaaact ggctaaatca tcaatgtcaa cagaaagtga tgcatcaata
    20881 atcattggaa gaattttttt tccgctcatg cctcctgctt ctattgttga agttgcaatt
    20941 acatccagtg tccctacttg ccagtaaact ttaggcaatt gctgccgagg catattaaat
    21001 ggctctgcaa tattttcaat gctaagcagt ggttccattg gggcatgttt gtctgctcca
    21061 agtgtccaca ttttatatgg cgtattcggt gcttctgtga taatgcgcag cgaatcggca
    21121 ttgcgttcaa ctacagcgcg aataccatca tctatccaac ccggcattct tatgggtgac
    21181 gtaggtcgca gctgaacaat aatatccggt ctatattgct cattttcaag gtgccatttc
    21241 aattgatgac taaaaacttc aaaatcagtg gtattgtcta acgcgaggtt agccggtcgc
    21301 ataaatggaa tttcagagcc atattgttgt gctattgcag caatgtcaag atcatcagta
    21361 ttgacaataa tacgtgtaat taattcactt tgttgagccg ccaaaatact atatgcaaga
    21421 agcggatgac cattgagtgg acgaatattc ttccctggta atcctttgct tcctcctctt
    21481 gccggaataa tagccagtac ttccatgtag tattaattac ctgacttaaa ttttagtttt
    21541 tttcgctgtg cctgttcaac aggtaatatg tcttcatgct tgtattgaag agcttgctgc
    21601 atatgatgta agtcgcgcac taatttttgc aatcccggaa cttccagact cgcagcgtga
    21661 tcagtgcctt tccaggtgcg gtctttggta aagtgacgtt ctatccatct tgcacccaat
    21721 gtatatgcag caatatcggc ggctattcct aaatggtgac cgctgaaacc tatttccttt
    21781 acgcgctcac caaattgttg atataattta ttgatctcca gcaaacatat ttctgaaaat
    21841 tgaatcggat agccgcttgt gcaactgtat aaaaccaatc tgttctttgc ggaattagtt
    21901 tgctcaaaaa aggaaaccat tttttcagtt tcgtctgatg tggtcatccc gacagatata
    21961 tgaatatcac cgttataggt gtcgcgcaat atttcgagta atgcaaagtt tgtgttgcag
    22021 gctgatggga ttttaataaa atcaggattt aatcctatca attcagaagc gctggtaata
    22081 tcccacaccg aagcactata tcctatgcct atgctctctg cataagattt tagatgtgcg
    22141 tgctgttcaa ccgtgaattc cagaaactca cgatgggcgc cataggtatc accatagctt
    22201 tgccatggct ccggatgcgg agcattatat tgattgttgt ccaataattc ctttggattt
    22261 cgtttttgaa atttcgcata atcagcacca cactcttttg caagtcggat gagctccttt
    22321 gcaatatcaa aatctcccat gtggttgcat cctatttcag caatcacttt tggcattcga
    22381 taatcaaatt gaaacatagg tcattttaca gcttcgcctc ctcagtcaca ttggtgctaa
    22441 gtcgaccatg aaaaaattac atttattcgg caacgccatt cgaccataat acaatggacg
    22501 aattcaatat gccgttttct ttatataaag caaatgtttg ctcattcact actgtggtga
    22561 gcactttgcg tttaattaaa gtttcagttt ttaaaaccca atattgcaat cgctccggat
    22621 ccacttcgcc aactatcatc aggtcgatat tgccggtatc ctttccttca gcataatctc
    22681 cggtgatcaa tgcatagtgt atttcaccga tctgcttaac aatattttct acgatcttat
    22741 caattcccaa atacttcctc accagactat tcaattccgg aaaaagcggg tgtttatcgt
    22801 ttgccttgta ttgaatggta tttccttcac catacgacac taaaaatccg gccttcgaca
    22861 gcttgttcag ttcaagtcgc actgcatttg tagattcatg catctccttc gccaaccccc
    22921 gtaaatgggc agtcgaccgc gtattgctga aaaacttcaa caataatttg atcctggtcc
    22981 tcgatgtgat caacgattct aacacagaaa cgggtaatga gtaacaaatt tactcatttc
    23041 aacaaaacca aaaaaaaatg tgaaacaccc tcctcccccc tctctccttt ttccttcctc
    23101 ctctctcctt tttcctctct cctcctcctc agaacaatct catttgcccc ggtcttctaa
    23161 acagcgaagt attataagta aacatcttcc tctccggcat atatttatcc ctcgacaacc
    23221 tgaaaagttt gtgtattgac tccgccaaat gcccatcgcc acgcattctc agcttccatc
    23281 tggaatcgtt gagttgcccc tggtgaactg ccctgatgtt gttcaataca cgctctgctt
    23341 tatcaggaaa agccttgtgt atccaatcgc tgaagatttg accaactgca ccattcaatc
    23401 gaaccatatt gaatcccgca tcgaaggccc catgttcagc tgctgcagcc agtagggcag
    23461 gaatctcgtg atcgttaagt cccggtatta caggtccgat cataataccc acaggaatat
    23521 gcttttcgct tagctttttc agaatatcca agcggttggc agcagtggaa gtgcgcggct
    23581 ccattgccct tcgcagcgat tcatccagcg tggtgacgct gatatatact ttaataaggt
    23641 catgctgtgc cagctcctcc agaatatcca aatcacgcag tatcaaacga ttttttgtga
    23701 taattcctgc cggattccta tatcgttgac atacctccaa caagcccctg gtgagccgca
    23761 atttgcgttc tacaggctga taacaatcgg tgttccctga aaaacttacg ggggcaggaa
    23821 cccagttttt gtcattaaac gccttttcca gcagttttgg ggcatccggc ttggtgataa
    23881 tgcgtgtctc gaaatccaat ccggcactaa aaccccagta ttgatgcgtg ttgcgggcat
    23941 aacaatacac gcagccatgc tcgcaaccct ggtatggatt taccgaaatt cccatgggtg
    24001 aatccggact gtcaacaaaa ttgacgatgc gtttagggta ttctgtaaaa aactgtgttt
    24061 cgcccggtgc aggccagctt tcgtcaagac cttcaatatg ctcctgagca tagttaatgt
    24121 cgtgataagg attaaaaggg ttgatctgtg aacccctgcc ttgaaaaaat tttttttcgt
    24181 ccatgcttgc aaattcgaaa aatgtcatta tttttgacaa ataaagtcat atttgctgac
    24241 attcacaaaa aagaaaagca catgtcaaac atagcaagta acctcaggca tctgcgcgga
    24301 gagcgcaaac ttagtcaaca agaattggcc gacatgatag gaatcggtcg aaccacgatt
    24361 gccaatattg aatcgaacct ggcaaatccg ggacataaaa ctttgttgga gatcgctaaa
    24421 gtttttaatg taaccataga cgagctcctg catgccgatc ttacaaaacg cattcttatt
    24481 caggagaagc cggctcaaca tgttgcattc cgaccggttg tagttactgt tgatgagctc
    24541 ggtcaggaaa atattgtgtt ggtagacacc aaggcagccg caggttatcc aacccgatat
    24601 ctcgagcctg aatattatcg cgaactaccg gcatttaaat taccgggtag cgattataga
    24661 aatggaacct tccgatgctt tcaggttgat ggcgatagta tgcaggatac cattcaacac
    24721 ggcgattttg tggtgggtag attttgcgac aatcatttca aagatgtgcg cgatggttat
    24781 atccatgtgg ttgttactaa tgataatgtc ctggtgaaaa gaattatcag tcgcgcagaa
    24841 tctgaacata aattatacct catcagcgat aatgatgcat atccggaaat agtagtcgat
    24901 attgatgatg tgcgtgaaat atggtttgtg aaaagtaaaa tttcaagcta tatgccggtg
    24961 aagaaaaatg atctcgataa agtgatcggt gatcttacca tgcaagtgca cttactgaaa
    25021 agcagaattg atcaactcga aaaaaactaa atcaattcgc aagtggagcg tagcgacatc
    25081 ccgacccctc gggattagca agttagttaa acaacacatc aacaaatcat acccggaact
    25141 acaagagcgg actgctgctg gaaaaaatat ttggtacata atttttcgct gcaaccgatc
    25201 ttccgccggg atttttaaat aacaaagctg ataccgaatg acatcgctca aaagagccgg
    25261 tgcaggggat tgtcggccta aaaaaagaaa aattatgatc gcaccaagta acattattac
    25321 caaccgccaa cgcattgcaa tcggcgaaga attgatctgg cagcaattga aagaaaaaag
    25381 agtttttggt ttgactttcg ctaggcaata tccattttat ttacaacaac cggatggtga
    25441 gcgattggta tactcagcag atttttattg tcatgatgca ggtgttgcag tacgcatagt
    25501 taactgcgat gccattaccg ctgagtataa tgagcaaatg gcctggtgta aacaacaatt
    25561 gcaaatgcgc ggaattttgg taattgaatt gccatgcgac gccattgcag atcttaaaca
    25621 ggttattgct gtaattcgac gagaattaaa atataagacg atacacgcat gaaaatctgt
    25681 agttgctttc cggagtaatt tcgatactgc ttatcaattt aagagctttt caaccaatag
    25741 gtaattacct atctttgtga aatgctacct gtatttacta atccggttca gattactcct
    25801 gagatacttg tcgggaacgg caaaccactt cttattgccg gaccatgtgt tattgaaagt
    25861 aaagcccttt gtctcc
//
LOCUS       DAOQHY010000023        25737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_837_length_25737_cov_5.737871, whole genome shotgun sequence.
ACCESSION   DAOQHY010000023 DAOQHY010000000
VERSION     DAOQHY010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 25737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25737
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_837_length_25737_cov_5.737871"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..1523)
                     /gene="atpD"
                     /locus_tag="PLL28_03360"
     CDS             complement(18..1523)
                     /gene="atpD"
                     /locus_tag="PLL28_03360"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNF68384.1"
                     /translation="MSGKGKIKQIIGAVVDVAFEEGSLPEILNALEVKRENGEALYLE
                     VQQHLGEDSVRTIAMDSTDGLVRGAEVIDLGKNISMPTGDAIRGRLFNVVGTAIDGMR
                     PVDSDVRYPIHRKPPLYEELSTEAEVLFTGIKVIDLIEPYAKGGKIGLFGGAGVGKTV
                     LIMELINNIAKGYSGMSVFAGVGERTREGNDLLREMIESNVIRYGEDFKHSMEQGGWD
                     LSKVDYSQLNQSQATLVFGQMNEPPGARARVALSGLTVAEYFRDGDPNEAKGRDILFF
                     IDNIFRFTQAGSEVSALLGRMPSAVGYQPTLASEMGLMQERITSTKRGSITSVQAVYV
                     PADDLTDPAPATTFSHLDATTVLNRKISELGIYPAVDPLDSTSRILSADIVGEEHYRC
                     AQRVKGILQRYKELQDIIAILGMEELSEEDKLVVSRARRVQRFLSQPFHVAEQFTGLK
                     GVLVPIEETIRGFNMIMDGEVDEYPEAAFNLVGTIDDAIEKGKKLLADAKK"
     gene            1879..2925
                     /locus_tag="PLL28_03365"
     CDS             1879..2925
                     /locus_tag="PLL28_03365"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNF68385.1"
                     /translation="MAQVDSTHTDTVHRSKGKFFPSPVLGYSPETRLYFGAGLVWYLP
                     PSKKYPNSNASVIKAVGVYTLNKQIESNISGESYFGDNLYKLNYTTNFFKFPDAFYGI
                     GNQNSPDDKESYNYDYFNLQVNGERLVFPKFFAGLKTFIETTKVYDVKEGGIFDTEDV
                     PGEDGGLNTGIGPWLTYDTRDNIYFPLKGLNADFSAVFHSKLTGSNYTYVDYMFEFSQ
                     FNKMMRDDVLGFNVYAQFVPGEAPFNRLAQLGGDRHMRGHYEGRYRDINYIAVQAEYR
                     ITFWKYFGVNIFAGTGEVAPDLNSFSTAGLHYSYGVGGRLFIVPEDKLSLRVDYGFDE
                     EGNGSLYVTFREAF"
     gene            2979..3731
                     /locus_tag="PLL28_03370"
     CDS             2979..3731
                     /locus_tag="PLL28_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF68386.1"
                     /translation="MNIEFNIFQHLGKFLIMIGRMFSRPERWRMYFKEMFRQMVDIGV
                     GSAFIVLLVSIFIGAVTAVQTAYQLYGTPFVPSSYIGLIVRNTIIFELAPTFTALILA
                     GKVGSNLASELGSMRVTEQIDALEIMGVNTESYLISTRVMAAIITIPILVSVAAYSGI
                     MGGYIATAAGSNYSVEDYVRGIRMYFDPFDVQLMFIKSFAFSFIVSSISCYMGYYVNG
                     GSVEIGRASTKAVVYSCIFILLADYVIAALLL"
     gene            3728..4501
                     /locus_tag="PLL28_03375"
     CDS             3728..4501
                     /locus_tag="PLL28_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNF68387.1"
                     /translation="MIEIRDISKSFGEQHVLKQVSAVFESGKTNLVIGISGSGKTVLM
                     KIMTGLITPDAGKVFFNDRDYYDLSRSEQKLLRREIGMLFQGSALFDSLTVEENVMLP
                     LNMFSNDTQKERLDRVNFCLDRVNLAGANKKYPGELSGGMKKRVGIARAIVLNPRYLF
                     CDEPNSGLDPRTSIVIDDLIIEITKEYNMTTIINTHDMNSVMEIGEKIVFLYKGVKDW
                     EGNKDEILQAKDENLNSFIFASDFLRDARDALKEKKGLL"
     gene            complement(4545..5564)
                     /locus_tag="PLL28_03380"
     CDS             complement(4545..5564)
                     /locus_tag="PLL28_03380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNF68388.1"
                     /translation="MIEIGIDSFAAKPSRLGISDAQAINELLERIVYADEVGLDVFGL
                     GEHYRKEFLDSASYMLLAAAASRTKNIKLTSAVTVLSAADPVRVFQNFATLDLISNGR
                     AEIVTGRGSFIESFPLFGYDLHDYDALFTEKLDLLLKIRDNEFITWEGAFRPALQHQP
                     VYPRPMQEKLPIWIGVGGTPASFARAGALGLPLMVAIIGGETRRFKPLVDLYRQAGAK
                     AGFAPEELQVGLHSLGYVANTKEEAIADYYPGYAEMFTKIGIERGFGPVTRGGFDAQN
                     GHYGALLVSDPEEVAHKIIRHSESLGGISRFTFQMDAGIPHEKLMQAIHLIGTKVKPI
                     LMDAK"
     gene            complement(5605..6165)
                     /locus_tag="PLL28_03385"
     CDS             complement(5605..6165)
                     /locus_tag="PLL28_03385"
                     /inference="COORDINATES: protein motif:HMM:NF041635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM3941 family protein"
                     /protein_id="HNF68389.1"
                     /translation="MQLEVRIKKQFLISGIIGSLVLCGIAFMILIYSTPGMSSPWRYV
                     VQALQVIIILFSIPNAILGIRRLIGSRIGLRISDEGVSDITVANAGRMYLWKEIKAVR
                     LHDANQVKIPFIAIELLDPDIFMHGKIGVARKILQTNIAKYGTPVVIRPTVLDIDPHI
                     LLDALREGLDEFTTSQVHNEQAEVIN"
     gene            complement(6298..7698)
                     /locus_tag="PLL28_03390"
     CDS             complement(6298..7698)
                     /locus_tag="PLL28_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNF68390.1"
                     /translation="MRNILFVIACMNAAIAPAQVIFSSKPVFGFAYQQETNDPFPVIK
                     EVTLGSPAFLANLMVGDHLLRINNADLRDISTDALTAILRDAPQMQNVITVQHVDATK
                     TEIKLNKANYITTEYESFRYTIFFDANSSPIYKEGENCLWTTTDCYNGYGYYVFDAYG
                     AFYGEFENGNRKEGLYYHPVFGKPTYYLGEFLNNEYEGLGTLQYLSDDSIVRVYKGNF
                     HNGAPFGQGEIHTADGRLEYTGGFNGWDYNGQGKYIFPDGTVKEGLWTNGQFADDIVM
                     PSTETPDNTGYTAEEIEKADKCRKAGLTEKEIRNFIMELRNPYSSDNSSSAGTTETLS
                     GIDYETSVWNEVMKNYGYGLLEEFDIGFSINEIGTCFTGCSTGQRRIMLLIPHVYGIQ
                     VSDLYVVTSRLSGDISDVNYEHFPFELISSDGDYTVYNCNIVASEIHSGCSYQWQVLS
                     EDGTRRFVRVLVFAIL"
     gene            7944..10154
                     /gene="katG"
                     /locus_tag="PLL28_03395"
     CDS             7944..10154
                     /gene="katG"
                     /locus_tag="PLL28_03395"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HNF68391.1"
                     /translation="MEQEKMSGGKCPFPHEMLKPVAGRGTRNQDWWPNQLKLNILRQH
                     SSLSNPMDPDFDYAKAFNSLDFKALKKDIEDVMTTSQAWWPADYGHYGPFFIRMAWHS
                     AGTYRIADGRGGGGSGTQRFAPLNSWPDNANLDKARLLLWPVKKKYGNKISWADLMIL
                     AGNCALESMGFKTFGFGGGREDVWEPEEDIYWGSEGKWLDDKRYSGDRDLENPLAAVQ
                     MGLIYVNPEGPHGNPDPLAAARDIRETFARMAMNDEETVALIAGGHTFGKTHGAADPG
                     KYVGPEPAAADIEMQSMGWKNTFGSGNGKETITSGLEGAWTTTPTKWSNNFFWNLFGY
                     EWELTKSPAGAQQWKPKYNMGADTVPDAHDPALRHAPIMLTTDLALRFDPAYEKISRK
                     FLDNPEAFADAFARAWYKLTHRDMGPVSRYLGPEVPNEVLIWQDPVPALDHPVINEQD
                     IAGLKSQILSLGLSNAALISTAWGSASTYRHSDKRGGANGGRIRLSPQRYWAVNNPTQ
                     LNAVLDGLHKIQSTFNAAQSNGKRVSMADLIVLAGCAAIEKSAAAGGHNVQVPFTAGR
                     TDASQEQTDVDSFAVLEPQADGFRNYAKNHFSMLAEEMLIDKAQLLNLTAPEMTALIG
                     GMRALDTNFDQSKHGVFTDRPGVLSNDYFVNLLDFNTTWQATNDHKDLFEGRDRVTGN
                     IKWTGTRVDLIFGSNTELRAIAEVYACEDGNAKFIHDFIAAWNKVMNADRYDLK"
     gene            complement(10227..10730)
                     /locus_tag="PLL28_03400"
     CDS             complement(10227..10730)
                     /locus_tag="PLL28_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68392.1"
                     /translation="MKTKVHITVIYEATLERAFKTPMLCDVTKVHTGYGLMPKIIDVV
                     NDSDWGKPGSSKGVIAAKSISFKGGLASTDHVLARVENDYWHICVDDFQAWMLGLYKF
                     EGIWETTQLEKGRVQVDYTYHLHANQPLFYPFVWLFSKLFWKRYMVHVTENIRSLIAN
                     DEPYMYK"
     gene            complement(10768..11220)
                     /locus_tag="PLL28_03405"
     CDS             complement(10768..11220)
                     /locus_tag="PLL28_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HNF68393.1"
                     /translation="MKSIFSPEVSNEVIARIEKLTPESKPLWGKMSVDKMLAHCNVTY
                     ELIYDNKHPKAGAFKQFLLKLFVKNAVVSEKPYKKNSPTAPEFKIVDPRIFADEKKRL
                     IDYINKTQQLGADYFDGKESNSFGKLNSTEWNIMFYKHLDHHLGQFGV"
     gene            11375..12763
                     /locus_tag="PLL28_03410"
     CDS             11375..12763
                     /locus_tag="PLL28_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68394.1"
                     /translation="MPRIQYLATCLLIVLVLPCSAQFSTIFDFTGDTTGSYPYYCKLL
                     EVDGQLYGTTANGGAFYDGTIFKINPDGSGFQKLIDFNEETTGSIGESALIYDGEFLY
                     GTARSGGDFNMGTIYKIKPDGSEFSKLHDFIGSDDGINPYGSLFFDGSFLYGMASGGG
                     AGGHGTIYKLKRDGTDFSSILAFDFGTSGNGPNGALISDGTYLYGMATSGGTGLYGTV
                     FKIKPDGSDFSVLTSMSDDPNGAYNYGSLVYDGSYLYGMTNNGGELNRGTIFKISTDG
                     TDYQKLHDFDDTTGAQPLGSLTLVGSTLYGLAELGGEFGKGTMFKLETDGSNFEKLYD
                     FNGINGELPISTLTNYNGALFGMTNTGGSKSQGVIFKYGEFTSEVTQYEHGKIHMYPN
                     PATEVIHIDPELLVYDKELPVQIINTSGQCVLATAISGSGTISVKSLPSGIYQLIISA
                     GGALYAETFVKQ"
     gene            12878..13756
                     /gene="sucD"
                     /locus_tag="PLL28_03415"
     CDS             12878..13756
                     /gene="sucD"
                     /locus_tag="PLL28_03415"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNF68395.1"
                     /translation="MSVLVGKNTRLVVQGFTGKEGTFHAEQMIAYGTNVIGGVTPDKG
                     GTKHLDRPVFNTVEDSVKKEGANTSIIFVPPAFAADAIMESADAGIQVIVAITEGIPV
                     QDMVRVKAYISNKSCTLIGPNCPGVITPEEAKVGIMPGFIHKKGSIGIVSRSGTLTYE
                     AVDQVTKAGMGQSTCIGIGGDPVPGTTTLDAIKLLMADPETEGIILIGEIGGTMEANA
                     ANWIKEHGTKPVVGFIAGQTAPKGRTMGHAGAIVGGKDDTAAAKMEIMRNCGIHVVES
                     PAVLGETMAMAMKGRI"
     assembly_gap    13860..13959
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13965..14711
                     /gene="fabG"
                     /locus_tag="PLL28_03420"
     CDS             13965..14711
                     /gene="fabG"
                     /locus_tag="PLL28_03420"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNF68396.1"
                     /translation="MGLLTNTTVLITGASRGIGEAMAMRFAEAGANVAFTYLSSEARA
                     QALEQKLSEMGVKAKGYKSNAGVYAEAEKLINDVIADFGNIDTLVNNAGITKDTLMLR
                     MSEEDWDAVMDVNLKSVFNLTKFAIKPMMKARKGSIINISSIVGLIGQAGQANYSASK
                     AGIIGFTKSIAKELGSRNIRCNAIAPGFIETDMTGELSEELKQTYFKSIPLGRFGQGK
                     EVADVAIFLASEMSSYVTGQVISVCGALHT"
     gene            14825..16921
                     /locus_tag="PLL28_03425"
     CDS             14825..16921
                     /locus_tag="PLL28_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68397.1"
                     /translation="MQLQIFSDYPWWFWFLCVLGGALYAAALYWRERRIAEVLPKQWW
                     IPAALRFIGATILLMLLLNPYMRSSTNETEKPIVVLLQDNSSSIAKGIGDTTAFLDAW
                     ESMATELSKDFDVRKYVFGETMEEDGENHFDQASTNISQSLLSLNNLYEQQHVAGVIL
                     ASDGIYNSGSNPVFIKNELNAPIYTVALGDTTRKKDVRILRTLHNNLVYLDDRFTVQA
                     DIEGIFCNGINISASISEINKNGNRNLGEQSVNAVGDQFNGSVKWEIAAQEPGIRHYR
                     INVRKVEGEFTTENNVQDIYIEVLDARQKILLLANAPHPDLQALRLSITQNKNYDVDV
                     QLAGEWKGNIADYDLVILHQLPSSTNTIPQVINEVQAKRIPTWFITGSQTSVVAFNKA
                     QSLLNINSNGQSANEVTALMDLQFNLFTIDDKTLGILPRLPALSAPYGQYTASKASQV
                     VAYQKIGSVATKTPLLLFSIPGGEKTAVLCGENIWKWRLYDYVLNNTQEASDEIIGKT
                     VQYLAAKNDKKQFRVSQARAVYTEQENIILDAELYNDTYELINTPDATITITDESGKD
                     FDFQFSKTGNSYTLSAGYFSPGNYSYKASTTFNGKTYTDAGAFSVSPVRLETINITAD
                     HRLMNQLAVQTGGSMAYPENMASLVDAIKNSAQAKPVMREITRTQSIIHLKWIFAVIL
                     LLLSVEWFIRKYSGGY"
     gene            complement(16954..17280)
                     /locus_tag="PLL28_03430"
     CDS             complement(16954..17280)
                     /locus_tag="PLL28_03430"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNF68398.1"
                     /translation="MTTKRSGFRQRLRQWKVPALLVNKYVFTLFVFGVWMTFFDQNNF
                     MRQYRRIAELNAVKSKTAYYESETAKAKKQLQALQNDQKALERFAREKYYMKKPNEDV
                     FVIIDK"
     gene            complement(17400..18683)
                     /gene="eno"
                     /locus_tag="PLL28_03435"
     CDS             complement(17400..18683)
                     /gene="eno"
                     /locus_tag="PLL28_03435"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNF68399.1"
                     /translation="MSNIIHIHARQILDSRGNPTVEAEVLTEFGAIGRAAVPSGASTG
                     IHEAVELRDEDPDVYLGKGVLKAVEHINESIVHELEGVDIFNQNLIDQIMIELDGTPN
                     KSKLGANAMLAVSLACAKAAASELSLPLYRYVGGVNANTLPVPLMNILNGGAHADNKI
                     DFQEFMIAPIGGDNFSDALRMGVEVFHHLKKILKNKGYSTNVGDEGGYAPNIQTNEEA
                     IETVLMAIESAGYVPGEDITIAMDAATSEFYNAEKGLYIFKKSDGRQMTPAEMVAYWA
                     EWAKKYPITSIEDGCAEDDWEGWKLMTQQLGNKIQLVGDDLFVTNVKRLSKGIKEHVG
                     NSILVKVNQIGTLTETIQAVDMAHRARYTSVMSHRSGETEDTTIADLAVALNCGQIKT
                     GSASRSDRIAKYNQLLRIEEDLGSMAVWPGNVFLR"
     gene            complement(18711..19811)
                     /gene="carA"
                     /locus_tag="PLL28_03440"
     CDS             complement(18711..19811)
                     /gene="carA"
                     /locus_tag="PLL28_03440"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNF68400.1"
                     /translation="MENFEKVPAVLLLADGTAFYGFSAGKIGTTTGEICFNTGMTGYQ
                     EIFTDPSYFGQILVMTNAHIGNYGTYSEDVESENIKISGLAVKNFTNQFSRRMTTTTI
                     QDYLLDEHLVAISDVDTRAIVRHIRSKGAMNCIISSETTDIEVLKTKLAETPDMNGLE
                     LASIVSTKTPYFIGNSTAEKKVLVYDFGVKKSILQNIADRGCYLKIVPANTPFEETTS
                     FDANGYFISNGPGDPASMGYAVDTVKKILAADKPLFGICLGQQLLALANGVSTYKMHH
                     GHRGCNHPVKNLETGLCEITTQNHGFSVREDELRANNNLEITHMNLNDQTVEGFRLKN
                     KPAFSVQYHPEATPGPHDARYLFDQFVKSMHV"
     gene            20072..21298
                     /locus_tag="PLL28_03445"
     CDS             20072..21298
                     /locus_tag="PLL28_03445"
                     /inference="COORDINATES: protein motif:HMM:NF025600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HNF68401.1"
                     /translation="MKKSTTLIMSMVCTVVTVRAQLTPEITSWITDAGSSNFHDIIYS
                     NVQQVQYTDADVFISCDAIAAYDVGPWDDPALGSNQNFVFRIPRLPQAADPTPLQPGH
                     IGVWSNGLSIFDIRSGQSYHNNNIWHVDAVAENAEVEPSCTGRIAENGEYYNYTAPAC
                     LFDPTQNTEHATILGYAFDGYPIYGAYAFENTDGSGNIVRMRSSYQLRNITLRNSLPD
                     GTMLSAEDQGPDVSNNYPLGRYLEDYIYVAGSGDLDEHNGRFCITPEYPQGTYAYFTT
                     INESGKPAFPYVIGGSYYGRVAEGNTGTFSGHQVIDESAATYTSVGADAAVLDMLVYP
                     NPTTDYIHVYIVPSYANDITATVRDISGRMVYQQNNMQPGVSYPIDARGLNSGLYFIE
                     LKSSDAYGMQKCMVRH"
     gene            complement(21360..22445)
                     /locus_tag="PLL28_03450"
     CDS             complement(21360..22445)
                     /locus_tag="PLL28_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNF68402.1"
                     /translation="MQDFLNHEFLHNTWRQWCWLIGVIIFVVIFKRYISKLISMLLFK
                     IVQRTAAGELSQEFLKRILKPLQYFIVLQTIYIGFHTLNYPFNKENEEGMALVQTILF
                     GAYRFLMILNIAWIVSRIGDFFVMVLKHRAEKTEDPWDDQIVVFLKDIIRLLVWTIAI
                     LSILGAVFGVNIYSIIAGAGIAGIAIAFAAQESLQNIFGSIAIFTEKPFVIGDLIDFD
                     GTQGTVVKVGFRSTRLRTIDTAYMTVPNKTIVNSKLSNLTRRTSRRVYFTIGLSHDTS
                     VEQLEAIRKEIYAFGMQHPKRNDEVNIGVTQYNTAAIEMLVEIHFEYVNWSAFVELRN
                     ELMLAIIQIVKKHGAVFAQQLAIAEKK"
     gene            22607..23767
                     /locus_tag="PLL28_03455"
     CDS             22607..23767
                     /locus_tag="PLL28_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HNF68403.1"
                     /translation="MITKLLTPVRISDFLQDWTIQPYHWARHIRMYTNEFPDTNNCLI
                     ALIGVNESRGSMQNSGCNTGADTVRRHLYQLAISEYPLQMIDAGDVIIGETPDETYQN
                     VKMVMEWFLGQQILPILIGGSQDITIGQYAGYEFLGQAVNLMVIDERVDLDDTELLPP
                     GRTWLQQIFRYDPKFLFNYSAIAYQTHFNSPEMLGAIDDLLFDTIRLGRLKENIQFAE
                     PEVRDADILSIDIGAIRGADAPGIAHATPNGLSGWEICQIMRYAGMSDKITSLGIYEY
                     NPMFDNQELTAKLVAQMIWYFVDGFYNRKSDYPIVSEKDFTKYIVNVAESDYDLTFWK
                     SMKSGRWWVEVPFIVQQRFERHQLVPCSYEDYENSLKNEVPEKWLRIFHKLS"
     assembly_gap    24020..24119
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(24443..25714)
                     /gene="murF"
                     /locus_tag="PLL28_03460"
     CDS             complement(24443..25714)
                     /gene="murF"
                     /locus_tag="PLL28_03460"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNF68404.1"
                     /translation="MITELYSRFSHAKGVTIDNRTISGGEMFFGLRGEKVNGGMFAGE
                     ALQKGASCCVVDDAQYATDSNIILVPDALLAMQELAAHYRSKWQFPVLALTGSNGKTT
                     TKELIAAVLSKKYRTYATKGNLNNHIGVPLTILNIGADCEFAVIEMGANHQGEIASYC
                     KYADPDFGLITNIGLAHLEGFGGIEGVAKGKSELYQYIIKKDGVIFWNSNQERLTDIA
                     KTYARTVRYGTNPEDYTIGIIDETATMATVVIDDEIKISSHLVGAYNADNIIAAACIG
                     KYFDVSYGEIQEAVENYIPANNRSEHREINGNYFILDAYNANPSSMRVALENFAKISA
                     QKKVIIIGDMLELGTYSADEHKQILDIALHSKFDHIVTVGPQFHHIRRDPVIACHNSD
                     EARQWFLAQQFREATILLKGSRGMKLEKIIQ"
BASE COUNT         7411 a         5384 c         5320 g         7422 t
ORIGIN      
        1 aaccctcaac tcctccttta ttttttcgcg tcagctaata attttttacc tttttcgata
       61 gcgtcgtcga tagtacctac gaggttgaat gctgcttcag ggtattcatc tacttcacca
      121 tccatgatca tattgaatcc gcggatagtt tcttcgattg gtacgagcac gcctttcaaa
      181 cctgtgaact gttcggcaac gtggaatggc tgagatagga agcgttgcac acgacgagcg
      241 cggcttacaa cgagtttatc ttcttcgctc aattcttcca taccgaggat ggcgatgata
      301 tcttgtaatt ctttgtagcg ctggaggata cctttcacgc gctgagcaca acggtaatgt
      361 tcttcaccaa cgatatcggc actcaagata cgtgatgtag aatccagtgg gtcaaccgca
      421 ggatagatac ctaactcgga gattttacgg ttcaataccg tagtagcatc caagtgcgag
      481 aatgttgttg caggagccgg gtcagtcaag tcatccgcag gaacgtaaac cgcctgaaca
      541 gaggtaatag aaccgcgttt tgttgaggta atacgctctt gcatgagacc catttcagat
      601 gccagagtag gctggtaacc tacggctgaa ggcatacgac caagaagggc tgatacttca
      661 gaacctgcct gagtgaaacg gaagatatta tcgataaaga acaggatatc acgtccttta
      721 gcctcatttg gatccccatc gcggaaatat tctgcaacag taagaccgga aagcgccacg
      781 cgagcacgag ctcccggtgg ttcgttcatt tgtccgaata caagcgttgc ctggctttga
      841 tttaattggc tataatctac tttgctaaga tcccaaccac cttgttccat agaatgtttg
      901 aaatcttcgc cgtaacggat aacgtttgat tcgatcattt cacggagcag gtcattacct
      961 tcacgagtac gctcgcccac acctgcaaac accgacatac cggaatatcc ttttgcgatg
     1021 ttgttgatca attccatgat caataccgtt ttacctacac cggcaccacc gaacagaccg
     1081 attttaccgc cctttgcgta tggttcaata aggtcgataa ctttaatacc tgtaaaaaga
     1141 acctcagctt cagttgacag ctcttcgtac agtggcggtt tgcggtggat aggataacgc
     1201 acgtcagagt caaccggacg cataccgtcg attgcagttc caaccacgtt aaacaagcgg
     1261 ccacggatgg catcgcctgt aggcattgaa atattttttc ccaagtcgat tacctcggca
     1321 ccacgcacaa gaccatctgt tgagtccata gcgatggtgc gaacgctgtc ttcaccaagg
     1381 tgctgttgaa cctctaaata gagggcttca ccgttttcac gtttcacctc tagggcgttg
     1441 aggatctctg ggagactacc ttcttcgaaa gctacatcga ctacggcgcc gatgatctgc
     1501 ttgatttttc ctttaccgga catgatttgt agactttatt atacggtttt aaaatccgcc
     1561 gcaaagatag gggtaaactc tgaaagtgtc aaagattgcg gcgttttata cccacatatt
     1621 tagggtttta atgtggattt tcgggaaaat gaaggtcaga ccgcatccat ggggctcagc
     1681 gttccgtata catgttgaag gatttctgtc atttaccctt taaaaactgc aattttgcat
     1741 cgccaataag attcaacaac cggttcacgt ttatttagcc attatcaacc tttgatgctc
     1801 ctctccccca gcccttgtcg ccaatttttc cgctttgcag gacttatggt tgcgttgtgt
     1861 agcatgcact ttcaaggaat ggcacaggtg gattcaaccc ataccgatac cgtacaccgc
     1921 agcaaaggca agttttttcc ttcgccggta ttgggttatt cgcctgagac tcgattgtat
     1981 ttcggtgccg gactggtatg gtatctacca ccatcaaaaa agtatccaaa cagcaacgca
     2041 tcagtgatca aggcagttgg tgtttatacg cttaataagc agattgaaag caatatcagt
     2101 ggcgagagtt acttcgggga taacttgtat aaactgaact acacaacaaa ctttttcaag
     2161 ttccctgacg ccttttacgg aatcggcaat caaaattcac cagacgataa ggaatcttat
     2221 aattatgatt acttcaactt gcaggtgaat ggtgagcgat tggtttttcc aaaattcttt
     2281 gcgggactga agacatttat agaaaccact aaggtttatg atgttaagga aggaggcatt
     2341 tttgataccg aagatgttcc gggtgaagat ggtggtttga acacaggtat tggtccatgg
     2401 ttgacatatg atacgcgaga caatatttat ttccctttaa aaggcctgaa tgctgatttc
     2461 tcggcagtat ttcattcgaa actaaccggc agtaattata cctatgtaga ttacatgttc
     2521 gaattcagcc agttcaacaa aatgatgcgc gatgatgtgc tcggattcaa tgtatatgca
     2581 caatttgttc cgggtgaagc ccctttcaac aggttagcgc aattgggcgg cgacaggcat
     2641 atgcgaggac attatgaagg acggtatcgc gatatcaact acattgccgt acaagctgaa
     2701 taccgcatta ctttctggaa atattttggg gtgaacattt ttgcgggtac aggtgaggta
     2761 gctcccgatc tcaattcatt ttcaacagca ggtttgcatt attcgtatgg tgtgggcgga
     2821 cgattgttta ttgtgcccga ggacaaactc agtttacgag tagattatgg atttgacgaa
     2881 gaaggaaatg gcagtttata tgtaaccttc agagaggcat tctaaattag cacatagata
     2941 tgtagtctgt gaagtaataa aagtataatt ttgaggcaat gaacatcgaa tttaatattt
     3001 tccaacatct gggcaaattt ctgatcatga tcggaaggat gttttcgcgc cctgaacgtt
     3061 ggcgaatgta ttttaaggaa atgttcaggc agatggtgga tattggtgtt ggttcagcat
     3121 ttattgtttt attggtttcc atttttattg gtgcggtaac tgctgtgcaa acggcatatc
     3181 aattatatgg tactcccttt gttccttcat catatatcgg attgatagtg cgcaatacca
     3241 tcatttttga attggcgccg acattcaccg cattaattct cgcaggaaaa gttggttcta
     3301 atctggcatc tgagttggga tcaatgcgtg tgactgaaca gatcgatgct ttagaaatca
     3361 tgggtgtaaa tacagaatct tacctgataa gcaccagggt gatggcagcc atcattacca
     3421 ttccaatttt ggtttccgta gcagcatact ccggaattat gggaggttat atcgctacag
     3481 cagccggttc aaattattct gtcgaagatt atgtgagagg tatcagaatg tatttcgatc
     3541 cttttgatgt gcagttgatg ttcattaaat cctttgcttt ttcatttatt gtctcttcta
     3601 tcagctgcta tatgggctac tatgtgaatg gtggctcggt agagatcggt cgcgccagca
     3661 caaaagctgt tgtttatagt tgcatattta ttttattggc agattatgtg attgcggcgt
     3721 tgttattatg atagagattc gcgacatatc aaaatcattt ggtgaacagc atgtgctgaa
     3781 gcaagtttct gccgtttttg aatcgggaaa aacaaacctg gttataggta tcagtggatc
     3841 cggaaaaacg gtgctgatga agatcatgac aggactcatc acacccgatg ccggcaaagt
     3901 attttttaac gacagagatt attatgactt gtcgcgttcc gaacaaaaat tattgcgcag
     3961 agaaataggc atgttatttc agggttcggc attattcgac agcctcactg ttgaggaaaa
     4021 tgtgatgcta ccgttaaata tgttcagcaa tgacacacaa aaggaaagac tcgatagagt
     4081 gaatttttgc ctcgaccgtg taaatcttgc cggtgcaaat aaaaagtatc ctggtgaatt
     4141 atcgggagga atgaaaaaac gtgttggtat tgcgcgtgca atcgttttaa atccacgata
     4201 cttattttgt gatgagccta actcaggtct cgatccacgc acttcaattg tgattgacga
     4261 tttaatcatt gaaatcacca aagaatataa tatgaccacc atcattaata ctcacgatat
     4321 gaatagtgtg atggaaattg gtgaaaagat cgtgttcctt tataaaggtg ttaaagattg
     4381 ggaagggaat aaagatgaga ttttacaagc taaagatgaa aacctcaact cctttatttt
     4441 cgcttcagac tttcttcgcg acgcacgcga tgctttaaaa gaaaagaagg ggctgcttta
     4501 gcatttggtg taaacaacaa ttattacatt ttcgttttca tacttcattt agcatccatc
     4561 aaaatcggtt tcacttttgt gccgatgaga tgaattgctt gcatcagttt ttcatgtggg
     4621 ataccggcat ccatttgaaa cgtaaatctg cttataccac ctaatgactc gctatggcga
     4681 atgattttgt gcgccacctc ctcaggatca ctcaccaaca atgcaccata gtgaccattt
     4741 tgggcatcga atccgcccct tgtaacagga ccaaaacctc tttcaatgcc aattttcgtg
     4801 aacatttctg cgtaacccgg ataatagtcg gcgatagctt cttccttggt atttgcaaca
     4861 taaccaagtg aatgtaatcc aacctgcaat tcctccggtg caaatccggc ttttgctccg
     4921 gcttgcctat acaggtcgac caatggttta aatctgcgtg tttcaccacc gatgatagca
     4981 accatcaaag gcaatcctaa tgcaccggcg cgtgcaaatg aagccggtgt tccgccaaca
     5041 ccaatccata ttggcaactt ttcctgcatt ggtcgcggat aaacaggttg atgttgaagc
     5101 gcaggtctga acgcaccttc ccacgttata aattcattat cgcgaatctt cagcagtaaa
     5161 tccaattttt cggtaaacaa ggcatcataa tcatgcaagt catatccgaa caaaggaaaa
     5221 gattcgataa aagatccacg gcccgtcact atttccgctc ttccatttga aatgagatcc
     5281 agagttgcaa aattttgaaa tactctgaca gggtctgctg cgcttaaaac cgtaactgca
     5341 cttgtcagtt ttatattttt tgtgcgagat gccgctgctg ccagtagcat gtatgatgca
     5401 gaatccaaaa attcttttct ataatgctcc cctaatccga atacatctaa tcccacttcg
     5461 tcggcataaa caatgcgctc cagtaattca tttatagcct gggcatctga aatgccaagt
     5521 cgtgaaggtt tagcagcaaa actatcaata ccaatttcta tcatgagaga agaaaagctt
     5581 ttatcggcaa attagttccc gacattaatt aattacttcg gcctgttcat tgtgaacctg
     5641 cgaggttgtg aattcatcca aaccttcacg caaggcatct aacaatatat gagggtcgat
     5701 atccaaaact gtaggcctga ttaccacagg agtaccatac ttggcaatat tggtttgcaa
     5761 tatttttctt gcaacgccga tttttccatg catgaaaata tccggatcca gtaattcgat
     5821 tgcaatgaaa ggaattttta cttggttagc atcatgtaat ctgactgcct tgatttcttt
     5881 ccataaatac atcctccccg cattagcaac agtaatatca gaaacacctt catcagatat
     5941 gcgcaatccg attctacttc caataagtcg gcgaataccc aaaatggcat tgggaatgga
     6001 gaacaaaata ataattacct gcaatgcctg aacaacatat cgccaaggtg aactcattcc
     6061 cggagtagaa taaatcaaaa tcataaacgc aataccgcaa agcaccagtg acccgatgat
     6121 gccggaaata aggaattgtt tttttatgcg cacttctaat tgcatggcac aaaggtagaa
     6181 tacataaatg atatattgtt ttctattggg ccggctgtat cagatgataa tcggcaattc
     6241 aaaattggta tgcatgtaaa catgaaagtt aaaatttaat gattttcctt taagtaatca
     6301 tagtattgca aataccaaca ctcgcacaaa tcgcctggtg ccgtcctcag atagaacctg
     6361 ccattggtat gagcatccgg agtggatttc ggatgcaact atattgcaat tgtatactgt
     6421 ataatcgcca tcactgctga ttaattcaaa tggaaaatgc tcataattga cgtcgctgat
     6481 atctcctgat aaacgagatg tcaccacata taaatcagat acctgaattc catacacatg
     6541 tggtataagc agcattatcc ttctttgccc ggtactgcaa ccggtaaaac aagtgccaat
     6601 ttcatttata gaaaatccta tatcaaattc ctctaagagt ccataaccat aatttttcat
     6661 tacttcattc catacacttg tctcataatc aatgcccgac aaagtttccg ttgtgccggc
     6721 agaggatgaa ttgtctgatg aatatggatt gcgtaattcc atgataaaat ttcgtatttc
     6781 cttttccgtc agaccggctt tgcggcattt atcggctttt tcaatttcct ctgcagtata
     6841 tccggtatta tcgggtgttt ctgtcgacgg cataacgata tcatcagcaa attgtccatt
     6901 cgtccacaaa ccttctttta ccgtgccatc aggaaaaata tatttcccct ggccattata
     6961 atcccatcca ttaaatccgc cggtatattc taatctgcca tcagctgtgt gaatttcgcc
     7021 ttgtccaaat ggtgctccgt tgtgaaaatt tcccttataa acacgtacta tgctatcatc
     7081 agacagatat tgcaaggttc ccaatccttc gtactcattg tttaaaaatt ctcccaaata
     7141 ataggtaggc tttccgaaaa ccggatgata gtagagtccc tctttcctgt ttccattttc
     7201 aaactctcca taaaatgcac cataagcatc aaatacatag tatccatatc cattataaca
     7261 atctgtagtc gtccacaaac aattctcacc ttccttataa atagggctac tgttagcgtc
     7321 aaaaaagata gtataacgaa atgactcgta ttcagttgtg atataatttg ctttattgag
     7381 tttgatttcg gtttttgttg catccacatg ctgaactgta attacatttt gcatttgagg
     7441 tgcgtcccgc aaaatggctg ttagtgcatc cgttgaaata tctcggagat cagcattgtt
     7501 tatcctcagc agatgatcgc cgaccatcaa atttgctaag aatgcagggc ttcccaatgt
     7561 cacttctttt attaccggaa atgggtcgtt cgtttcttgt tggtaggcaa acccaaaaac
     7621 aggtttactg ctaaaaatta cctgtgccgg agcaattgca gcgttcatgc aggcaatgac
     7681 gaataatatg tttcgcatgt gtttcatttg aactagatct ccaatctagc tataaatttt
     7741 ggtccagaac ggtaagttag ttacaatatc aagtttcagg taaggaaact gcattctaca
     7801 ctgccaaaag tgattgccgc gaatgtgatt gcatgtagga atttatttcg gcagaaattt
     7861 gtagtgtacg taagtcatac cgcagaaccg aaatgttcat taggtttgca ggataatgca
     7921 aatagcagaa aatcaaacat catatggaac aagaaaaaat gagcggagga aaatgtccct
     7981 ttccgcatga aatgttgaaa cccgtggcag gtcgaggaac ccgcaatcaa gactggtggc
     8041 cgaatcagct aaaattgaac atattacgtc agcattcatc gctttcaaac ccgatggatc
     8101 ctgattttga ctatgcaaag gcttttaata gtctggactt taaggcattg aaaaaggata
     8161 ttgaggatgt tatgaccacc tcacaagcat ggtggccggc agattatggt cattacggcc
     8221 catttttcat tcgcatggct tggcacagcg caggcactta tagaattgcc gatggtcgcg
     8281 gtggtggtgg ttccggcacg cagcgattcg cgccattgaa cagctggccc gacaatgcga
     8341 atttggataa agcgcgactg ttattgtggc cggtgaaaaa gaaatacggc aataaaattt
     8401 cctgggctga tttgatgata ttggcaggaa attgcgcgct ggaatccatg ggattcaaaa
     8461 cattcggttt cggtggcgga cgtgaggacg tatgggaacc tgaagaagat atttattggg
     8521 gatcagaagg aaaatggctt gacgataaac gctattccgg cgatcgtgat ttagaaaatc
     8581 cgcttgctgc tgttcaaatg ggattgatct atgtaaaccc tgaaggtccg cacggcaatc
     8641 ctgaccctct tgctgcggca cgcgatatca gagaaacatt tgcgcgcatg gccatgaatg
     8701 atgaagaaac cgttgcgctc attgccggtg gtcacacctt cggtaaaaca catggtgccg
     8761 ccgatcccgg aaaatatgta ggccctgaac ctgctgctgc agatattgaa atgcagagta
     8821 tgggatggaa aaataccttc ggctccggta atggtaaaga aacgataacc agtggattgg
     8881 aaggtgcatg gaccaccaca cctaccaaat ggagtaacaa tttcttttgg aatttattcg
     8941 gctatgagtg ggaattaacc aagagcccgg caggtgcaca acagtggaaa ccaaaataca
     9001 atatgggtgc cgacacagtt cctgatgcgc acgatcctgc attgcgtcat gcaccaatta
     9061 tgttgaccac agacctggcc ttgcgatttg atccggcgta tgaaaaaata tcaaggaaat
     9121 ttttagataa tccggaagct tttgctgatg catttgctcg tgcctggtat aaattaacac
     9181 acagagatat gggccctgtt tcaagatatc ttggccctga agtgccgaat gaggtactaa
     9241 tttggcaaga ccctgtacct gcactcgatc atcctgtgat aaatgaacaa gatattgccg
     9301 ggttaaaatc acaaatattg tcactgggtt tgagcaatgc cgctttaatt tccactgcat
     9361 gggggtctgc atcaacatac cgtcattcag ataaacgagg aggagctaat ggtggcagaa
     9421 ttcgtttgtc accgcagcgc tattgggcag taaataatcc aactcaattg aatgctgtac
     9481 tcgatggttt gcataagatt caatctacat ttaatgctgc tcaaagcaat gggaaaagag
     9541 tatcgatggc tgatttaatt gtgttggcag gatgcgcagc gattgaaaaa tcagcagctg
     9601 ccggtggtca taatgtgcag gtgccattta ctgccggaag aacagatgct tcacaggagc
     9661 aaacagatgt agattcattt gcagtgcttg agccacaagc agatggtttc cgcaattatg
     9721 ctaaaaacca tttcagcatg ttagcagaag aaatgctcat cgacaaagcg caactgttaa
     9781 atcttacagc gcctgaaatg accgcattga ttggtggtat gcgcgcactc gatacaaatt
     9841 tcgatcaatc taaacacggt gtattcactg atcgcccggg tgtattgagt aacgattact
     9901 tcgtaaatct gcttgatttc aacactacct ggcaggcaac aaatgatcat aaagatctat
     9961 tcgaaggtcg cgaccgtgta accggaaata ttaagtggac aggaacaaga gtagatttaa
    10021 tttttggctc gaatacagaa ctacgtgcta tcgctgaagt atatgcttgt gaagatggca
    10081 atgcaaaatt cattcacgac tttattgcag catggaacaa ggttatgaat gccgacagat
    10141 atgatttgaa ataaaagcta tctgcttgaa taataaaaaa ggtgatctat tgtttaggtc
    10201 accttttttt tatttaatat caggaatcac ttatacatat aaggctcatc atttgctatt
    10261 aagctgcgaa tattctcggt aacgtgtacc atatatcgct tccaaaacaa cttagaaaac
    10321 aaccatacaa acggataaaa aagcggttga tttgcatgga gatgataggt ataatccacc
    10381 tgcaccctac ccttctctaa ttgcgtagtt tcccaaatac cttcaaactt gtacaaacca
    10441 agcatccagg cttgaaaatc atccacacaa atatgccagt aatcattttc caccctggcc
    10501 aatacatgat ctgtagatgc aagtccgcct ttaaaactta tagatttggc agcgatgaca
    10561 ccttttgaag aaccgggttt accccagtcg ctatcattaa ccacatcaat tattttaggc
    10621 atcaatccat aacctgtgtg cactttcgtt acatcgcata acattggtgt tttgaaggcg
    10681 cgttctaaag tggcttcata tataacggta atatgaactt tcgttttcat gacgcaacct
    10741 ttatctgtga taacataatt aactgaatca aaccccaaac tgccccaagt gatgatcaag
    10801 atgcttgtag aacatgatat tccattctgt agaattcaat tttccaaagg aattcgattc
    10861 ttttccatca aaataatctg cacctaattg ttgcgtttta ttgatatagt cgatcagtcg
    10921 ctttttttca tcagcaaaaa tccgtggatc tacaattttg aattcaggtg ctgttggtga
    10981 attcttttta taaggctttt ctgatactac agcatttttt acgaacaact tcaacagaaa
    11041 ctgcttaaat gcacctgctt tcggatgctt attgtcataa atcaattcgt acgtaacatt
    11101 gcaatgagcc aacattttat ctaccgacat ctttccccat agtggcttcg attccggtgt
    11161 caatttttca attctggcaa taacttcatt tgaaacttca ggtgagaata tacttttcat
    11221 aactataatt ggcgatttaa tattttcagc aaagttaagg caattcacaa aactgtcaat
    11281 aattgattta aaagatcaaa aattgaaatg cccctccccc aatgtcggta ttgtgccttc
    11341 attcaaatcg attgaccttt gtaaaaacga cattatgcca cgtattcaat accttgcaac
    11401 ttgcctgctt atagtcttag tgctaccatg tagtgctcag ttttcgacca tttttgattt
    11461 taccggtgac accacaggat cttatccata ttactgcaag ttactagaag ttgatggtca
    11521 attatatggc actacagcaa atggaggtgc gttttatgac ggtacgatat ttaaaataaa
    11581 tccggatggc tccggctttc aaaaattgat agacttcaat gaagaaacta ccggctcaat
    11641 tggcgaaagt gcgcttattt acgatgggga atttttatac ggcacagcgc gttccggtgg
    11701 cgatttcaat atgggtacta tttataaaat caaacctgat ggcagtgagt ttagtaagtt
    11761 acatgatttc ataggaagtg atgatggaat aaatccttac ggcagcttgt ttttcgatgg
    11821 ctcttttctt tatggaatgg caagtggtgg aggcgcagga ggtcacggta ccatttataa
    11881 acttaaaaga gacggcaccg atttttcctc aattttagca tttgattttg gcacctccgg
    11941 caatggaccc aatggtgcac ttatttctga tggcacctat ctatatggca tggccacatc
    12001 gggtggtaca gggctatatg gaacagtttt caaaatcaaa cccgacggca gcgatttttc
    12061 agtgttgaca tctatgtctg acgatccgaa tggtgcctat aactatggtt cattggtgta
    12121 tgatggcagc tatttatatg gcatgacaaa caatggaggt gaattaaacc ggggaaccat
    12181 ttttaaaatc tcaacagatg gcaccgatta tcaaaaacta catgattttg acgacactac
    12241 aggtgcgcaa ccattaggct ctcttactct ggtaggatca actttatacg gattggctga
    12301 attaggcgga gaattcggaa aaggcaccat gtttaaacta gaaacagatg gcagcaattt
    12361 tgaaaaatta tatgatttca atggtataaa cggcgagttg ccaattagca cattgacaaa
    12421 ttataatggt gcacttttcg gcatgacaaa tacaggaggt tcaaaaagcc agggtgttat
    12481 ttttaaatat ggtgaattca cctcagaagt gactcagtat gaacatggaa aaatccatat
    12541 gtatcccaat cctgcaaccg aagtaatcca tattgatccg gagcttctgg tttatgataa
    12601 ggaattgcct gttcaaataa taaatacctc cggtcaatgt gttttggcaa ctgctatatc
    12661 aggttccggt acaatttctg tgaaaagtct cccatcaggg atctatcagc tcattatatc
    12721 cgcaggggga gcattgtatg ctgaaacctt cgtcaaacaa taacatttac accatgtact
    12781 tttgttgaag aagcaattgg gacaaaacag ttccaaagtt ttcacaatac attacactat
    12841 cggtgaaaca attctcaaat cgtcttatct tcgcggcatg agcgttttag ttggaaaaaa
    12901 cacccgttta gtggttcagg gctttaccgg taaggaaggc acctttcatg cagaacagat
    12961 gattgcttat ggcaccaatg taattggcgg cgtaacacct gataagggcg gaaccaagca
    13021 tttggaccgt ccggtattca atactgtaga ggattccgta aaaaaagagg gagccaacac
    13081 ttccattatt ttcgttccac ctgcctttgc tgccgatgcc attatggagt ctgcagatgc
    13141 aggtattcaa gtgattgttg ccattaccga aggtattcct gttcaggata tggttcgtgt
    13201 aaaagcgtat atcagcaata aatcatgcac cttgatcggc cctaactgcc ccggggtgat
    13261 cactcctgag gaagcaaaag tgggtatcat gcccggtttc atccataaaa aaggctctat
    13321 cggcattgtt tcccgctcag gaacattgac atatgaggct gtagatcagg ttactaaagc
    13381 cggaatggga caaagcactt gtattggtat tggtggcgac cctgttccgg gaactactac
    13441 acttgatgct atcaaattat taatggctga ccctgaaact gaaggaatca tattgattgg
    13501 tgagatcggt ggaactatgg aagcaaacgc tgcaaactgg atcaaagaac atggcaccaa
    13561 gcctgttgta ggtttcattg cgggtcaaac tgctccaaaa ggtcgcacca tgggccatgc
    13621 cggagcaatt gtcggcggaa aagatgatac tgctgccgca aaaatggaaa tcatgcgcaa
    13681 ttgcggtatc cacgtagtag aaagccctgc agtacttggc gaaacaatgg ccatggcaat
    13741 gaaaggcaga atttaacatc aaaagcaaac ccaaaaaatc gcaacaggtt tcaaaacacc
    13801 caattagtaa ctgatacttg gctcaagcta tcaaacaaca aatacccgca aatagcgagn
    13861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna acctatggga cttctaacaa
    13981 ataccactgt tttaatcacc ggtgcctcac gtggaatcgg cgaagcaatg gcgatgcgtt
    14041 ttgctgaggc cggtgccaat gttgcattta cctatttaag cagcgaagca agagcgcagg
    14101 cattggagca gaaactttct gaaatggggg tgaaggctaa agggtataaa agtaacgctg
    14161 gagtttatgc agaagccgaa aaattgatca atgatgtgat agctgatttt ggcaatatcg
    14221 acaccttggt aaataatgca ggcattacta aagacacgtt aatgttgcgt atgtcggagg
    14281 aagattggga tgcagttatg gatgtgaatt tgaaatcagt attcaatctc accaaatttg
    14341 ccatcaagcc aatgatgaag gcacgcaaag gcagcatcat caatatttcc agtattgtag
    14401 gtttgatcgg acaagcaggt caagcaaact attctgcatc taaagcaggt attataggtt
    14461 ttacgaaatc cattgcaaaa gaactcggtt ccagaaatat tcgttgtaat gccattgcgc
    14521 cgggatttat tgaaacagat atgaccggcg aattgagcga ggaactcaaa caaacttatt
    14581 ttaaatctat tccactggga cgcttcggac aaggaaaaga agtggctgat gttgccattt
    14641 tccttgccag tgaaatgagt agttatgtta ccggacaggt aatcagtgtt tgcggtgcct
    14701 tacatacctg atctgaaaaa gattacggta acgaatctta acaacattta ctacaactaa
    14761 ctcgggcaat tactcgttgt atttacctta tattcgcaca tagaagcgga tactccggca
    14821 atttatgcag ttacagattt tctctgatta cccctggtgg ttttggtttt tatgtgtact
    14881 tggtggtgca ctatatgccg ctgcattata ctggcgtgaa cgacgtatag ctgaagtgct
    14941 gccaaaacaa tggtggatac ccgcagcatt acggtttatt ggcgctacaa ttttgttgat
    15001 gttgctgctc aatccttata tgcgttcatc tactaatgaa acagagaaac ccattgtcgt
    15061 tttgttgcag gataattctt ccagtattgc aaaaggcatt ggcgatacta ctgctttttt
    15121 ggatgcatgg gaatctatgg caactgaatt atcaaaagat tttgacgtta gaaaatatgt
    15181 attcggtgaa acaatggagg aagatggcga aaatcatttc gatcaggcat ccactaatat
    15241 ttcccaatcg ctgttatcac tcaataattt gtatgagcaa caacatgtgg caggagtgat
    15301 attggcttct gacggtattt ataatagtgg aagcaatccg gtatttatca aaaatgaact
    15361 caacgcccca atttataccg ttgcgctggg agatactaca cgaaaaaaag atgtgcgcat
    15421 tcttcgcact ttacacaata acctggtgta tctcgatgat cgttttactg tgcaggctga
    15481 cattgaaggg attttctgca atggtataaa tatcagtgct tcaatttcag aaattaataa
    15541 aaacggtaat cgaaacttag gtgaacaatc tgttaatgct gttggtgacc aattcaatgg
    15601 aagcgtaaaa tgggaaattg ctgcacagga gcccggaatt cgtcattatc gaataaatgt
    15661 gagaaaggtg gaaggtgaat tcaccacaga aaacaacgtg caggatattt atatcgaagt
    15721 actcgatgcc cgacaaaaaa ttctgttact cgccaacgca ccacatcccg atctgcaagc
    15781 cttgcgttta tcgatcacgc aaaacaaaaa ttatgatgtc gatgtgcagc ttgcgggcga
    15841 atggaaaggc aatattgccg actacgacct tgtcatttta catcaattgc cttcttccac
    15901 aaatactatt ccacaggtga taaatgaggt acaggcgaag cgtattccta cttggtttat
    15961 tacaggcagc caaacatcag ttgttgcttt caacaaggca cagtcgctgc tcaatatcaa
    16021 tagcaatggg caatctgcaa atgaggttac ggcgttaatg gatttgcaat tcaatctctt
    16081 caccattgat gataaaacgc ttggcatcct cccaagattg ccggcactaa gtgcacctta
    16141 cggtcaatac acggcatcaa aggcatcgca ggtggtggcc tatcagaaaa ttggatccgt
    16201 ggctactaaa acgccattgt tattgtttag cattccgggt ggagaaaaaa ccgccgtatt
    16261 atgtggagaa aatatttgga aatggcgctt gtatgactac gtgcttaata acacacaaga
    16321 agcttctgat gagatcattg gtaaaacagt gcaatacctt gctgctaaaa acgacaagaa
    16381 acaattccga gtaagtcaag ctcgcgctgt ttatactgag caggaaaata ttattcttga
    16441 tgcagaatta tataatgaca cttatgaatt gattaatacg cctgatgcca ccattaccat
    16501 tacagatgaa tccggaaaag attttgattt tcaattttcc aaaaccggaa atagttatac
    16561 tttaagtgca ggatattttt caccaggcaa ttacagttac aaagcatcca caactttcaa
    16621 tggcaaaaca tatactgatg ccggagcttt tagtgtgtcg cctgtaagat tggaaactat
    16681 caatattact gccgaccatc ggttaatgaa tcaacttgct gttcaaaccg gaggcagtat
    16741 ggcctatcct gaaaatatgg catcgctggt tgatgcgata aaaaacagtg cgcaagcaaa
    16801 accggtgatg cgcgaaatca ctcgcacaca atcaattatt catttgaaat ggatatttgc
    16861 cgtgatattg ttattactca gcgtagagtg gttcatccga aaatacagcg gaggatattg
    16921 acaaataaca aatagccggt aataattaat gaattatttg tctatgatga caaatacatc
    16981 ttcattcggt ttcttcatgt agtatttttc acgtgcgaaa cgctccagag ctttctgatc
    17041 attctggagt gcctgcaatt gcttttttgc ctttgctgtt tcgctttcgt aataggctgt
    17101 tttacttttc actgcattta attcagcaat acgcctatat tgcctcatga aattattctg
    17161 atcaaaaaat gtcatccaca cgccaaatac aaacagggta aacacgtatt tatttacgag
    17221 cagcgcaggc actttccatt gcctgagtcg ttggcgaaaa cctgaccttt ttgttgtcat
    17281 gacctgataa agatacgtgg aggttacata ccataaacgc cggtaaataa aaaaaccgtg
    17341 tggattgttc acaaaaaccc acacggcaag ttaaattcgg ccttaagtcc cggcaaatgt
    17401 taacgcagga acacatttcc cggccaaaca gccatgcttc cgaggtcttc ctctatgcgc
    17461 agcagctggt tatattttgc aatcctgtcg cttcgcgaag cagaaccggt tttaatctga
    17521 ccgcaattga gtgccaccgc aagatcagct atcgtagtat cttctgtttc cccgctgcgg
    17581 tgactcatca ctgacgtata tcttgcgcga tgtgccatgt ctacagcctg tatcgtttcc
    17641 gttaacgtgc cgatctggtt tactttcact aaaattgaat taccgacatg ttcttttatc
    17701 ccttttgaaa ggcgttttac attcgtaaca aacagatcat cgccaacaag ctgaatttta
    17761 tttccaagct gttgcgtcat caatttccag ccttcccagt catcttcagc gcagccatct
    17821 tcaatagaag taataggata tttcttcgcc cattctgccc aataagctac catttcggca
    17881 ggcgtcattt ggcgaccgtc agatttttta aagatgtaaa gtcctttctc cgcattataa
    17941 aattcgctgg tagcagcatc catggcaatg gtgatatctt caccgggaac atatccggca
    18001 ctttctatcg ccattaatac tgtttcaatg gcttcttcgt ttgtttgaat attcggagcg
    18061 tatccacctt catcgccaac attggtagaa taacctttgt ttttaagaat ttttttcaag
    18121 tgatgaaaaa cctcaacacc catgcgtaat gcgtcagaaa aattatcacc accaataggt
    18181 gcgatcataa attcctggaa atcaatttta ttatcggcat gcgcaccacc attgagaata
    18241 ttcatcaatg gaacaggaag tgtattggca ttcacaccgc cgacatatcg atataagggt
    18301 aaactcaatt cagaggccgc agctttggca cacgccaatg atactgcaag catggcattg
    18361 gcaccaagct ttgatttatt cggtgtgcca tctaattcaa tcataatttg atcgatcaga
    18421 ttttgattga aaatatctac gccttcgagt tcatgcacaa tagattcatt gatatgttct
    18481 actgccttca acacaccttt ccccagataa acatcaggat cttcatcgcg cagctccact
    18541 gcctcatgta ttccggtaga tgctcccgat ggaacggcag cgcgaccaat tgcaccaaat
    18601 tctgttaata cttctgcttc aactgtaggg tttccacgag agtcaagaat ctgacgtgcg
    18661 tggatatgaa taatattgct catgatagat aaagttcttt aattgctgat ttaaacatgc
    18721 atggatttca caaattgatc gaacagataa cgtgcatcat gcggaccggg tgttgcctca
    18781 ggatggtatt gcacactgaa ggcgggttta ttttttaaac gaaatccctc caccgtttga
    18841 tcatttagat tcatatgtgt gatctccaga ttattattgg cacgcaactc atcttcacgc
    18901 acactgaatc catgattttg agtggtgatt tcacataatc ctgtttcaag atttttcacc
    18961 ggatgattgc atcctctatg tccatggtgc attttatatg tagacacgcc attggcaagt
    19021 gccaataatt gttgtccgag acaaataccg aataaaggtt tatctgctgc aagaattttc
    19081 ttcaccgtat caacggcata acccatactt gcaggatctc ccggaccatt agaaataaaa
    19141 tatccgttgg catcaaaact tgtagtttct tcgaatggtg tatttgcagg cactattttg
    19201 agatagcaac cgcggtcggc aatattttga agtatgcttt tcttcacacc gaaatcgtat
    19261 accaacacct ttttttctgc tgtgctgttg ccgataaaat atggtgtctt tgttgaaacg
    19321 atgcttgcca gttccagacc attcatatca ggcgtttctg ccagctttgt ctttaacacc
    19381 tcaatatcag ttgtttcgct ggagatgatg cagttcatgg cgcctttact gcggatatga
    19441 cgcacgatgg ctctggtatc tacatcgcta atagccacaa gatgttcatc gagcaggtaa
    19501 tcctgtatcg tggtggttgt catccgtcgg ctaaattggt tggtaaagtt tttcaccgcc
    19561 aaaccggata tcttaatgtt ttcgctctca acatcctcag aataggtgcc gtaattacca
    19621 atatgtgcat tggtcatcac caggatctgt ccaaagtaac tcggatcggt aaaaatctcc
    19681 tgatagcccg tcattcctgt gttaaagcag atttcgccgg tagtggtgcc gattttgccg
    19741 gcgctgaacc cgtaaaaggc agttccgtca gctaagagca gcacggccgg aactttctcg
    19801 aagttttcca ttaaagtgca aagatactag ccgcgactgt gactaccata tcaagtttca
    19861 cataaagtgc cataaaatcg gggattattc acactgtgtt gataaacacc ttgcaaccga
    19921 gcttgattat acatcgtatt tctgatgtcg aatcctaaca attgtatcat ttaatgaaca
    19981 aacttatgaa acacatggta ttacgtgaat tccctccatc gaaggagtgt ggtaatttta
    20041 agtccggttc aagttatcta aagttcaacg tatgaagaaa agcactacac tcatcatgtc
    20101 catggtgtgt accgtcgtca cggttcgcgc ccaactgaca ccggaaatca ccagctggat
    20161 caccgacgca ggatcctcca attttcatga catcatttat tccaatgtgc agcaagtgca
    20221 atacaccgat gctgatgtat ttatcagttg cgatgccatt gcagcctatg atgtaggacc
    20281 ttgggatgac cccgcacttg gttcaaatca aaattttgtt tttcgtattc cgcgcttacc
    20341 gcaagctgca gaccctaccc cactacaacc gggtcatatc ggtgtttgga gcaatggtct
    20401 gagcattttt gatatacgaa gcggacagag ttatcacaac aacaatattt ggcatgtaga
    20461 cgccgtagcc gagaatgcag aggtagaacc atcatgcaca ggtcgcattg ccgaaaatgg
    20521 ggaatattat aattataccg ctcctgcctg tcttttcgat ccaacacaaa atacagagca
    20581 cgcaacaata ctaggttacg cctttgatgg ttatccgata tatggtgcct atgccttcga
    20641 aaataccgat ggcagtggca atattgttcg catgcgctcg agctatcaat tgcgaaatat
    20701 cactttgaga aattcgctcc ctgatggtac gatgctttct gccgaagatc agggacccga
    20761 tgtcagtaac aactacccat taggtcgtta ccttgaagat tatatttacg ttgcaggctc
    20821 cggtgatctg gatgaacaca atggtcgatt ctgcataaca cctgaatacc ctcaaggcac
    20881 ttatgcctat tttacaacga taaatgaatc aggtaaaccc gctttccctt atgtgatagg
    20941 cggaagttat tatggacgcg ttgccgaagg gaataccggt acattttcag gccatcaggt
    21001 tattgatgaa tcagcagcta cctatacatc tgttggcgct gatgccgctg tattagatat
    21061 gcttgtttat ccaaatccga caacggatta tatacatgta tatattgttc cgtcttatgc
    21121 caacgatatt acggctaccg ttcgcgatat cagtggacgc atggtgtatc agcaaaataa
    21181 tatgcaaccg ggagtaagtt atccaatcga tgcacgcgga ttgaatagtg gcttatactt
    21241 cattgaactt aagagcagcg atgcttatgg catgcaaaaa tgcatggtta gacattaaag
    21301 agcgtcgcta atttcctgac tagaatttca accgggtagt taaggctatc cggttttttt
    21361 tatttctttt ctgcaatagc caattgctgc gcgaagacgg ctccgtgttt tttaacaatc
    21421 tgtatgattg ccaacatcaa ttcgtttcgc aattccacaa atgccgacca gtttacatac
    21481 tcaaaatgaa tttcaacaag catttcaatt gcagcggtat tgtattgtgt tacaccgata
    21541 ttcacctcgt cattccgctt aggatgctgc ataccgaaag cataaatttc tttgcgaata
    21601 gcttccaact gttctacaga agtatcatgc gataaaccga ttgtaaaata cacgcgccgt
    21661 gaagtacgac gagtgaggtt actgagtttt gaattgacta tggttttatt cggcacagtc
    21721 atataggctg tatcgatagt tctcaatcgg gtagaacgaa agcctacttt caccactgtg
    21781 ccttgcgtgc catcaaaatc aatcagatcg ccaattacaa atggtttctc tgtgaatatg
    21841 gctatgctgc caaaaatatt ttgcaggctc tcttgtgctg caaaagcaat ggcaattcct
    21901 gcaattcccg ctcctgcaat gatgctataa atatttacac caaatacggc gccaagaata
    21961 ctaagtatcg caatggtcca aactaataat cgaatgatat ccttcaagaa aacaacaatc
    22021 tggtcatccc atggatcttc agttttttct gcgcgatgtt tcaacaccat cacaaaaaaa
    22081 tcgccgatgc gtgaaacgat ccaggcgata ttcaatatca tcaaaaagcg atatgcacca
    22141 aacagtattg tctgtactaa tgccataccc tcttcatttt ccttattgaa tggataatta
    22201 agggtatgga atccgatata aatagtctgc aatacaataa aatattgcag cggtttgaga
    22261 atgcgtttta aaaattcttg cgacaactca ccggctgctg tacgttgaac tattttaaac
    22321 aacaacatac tgatgagttt cgagatatag cgtttaaata tcaccacaaa gatgatcacg
    22381 ccaatcagcc agcaccactg tcgccaggtg ttgtgtaaaa attcgtgatt gagaaagtct
    22441 tgcatgcgag ttgcctttat cactgcaaaa atatatacta gatcgcatct tggaacatat
    22501 tttgagtttt gattggttta caggatgacc gaataattag acaaaatgcg cctttactgt
    22561 tgccaacacg gtaattaatt cagcaaaaag gaggcaattt taacgcatga tcacgaagct
    22621 gctcactcct gttcgtattt cagattttct tcaagactgg accatacagc cctaccattg
    22681 ggcacggcat atccgcatgt ataccaacga atttccggat acgaataatt gccttatcgc
    22741 attgattggt gtaaatgaaa gtcgcggtag catgcaaaac agcggatgca atacaggagc
    22801 agataccgtt cgtcgccatc tatatcaact cgctatcagc gaatatcccc tgcaaatgat
    22861 cgatgcaggc gatgtaatta tcggcgaaac gcccgacgaa acctaccaaa atgtgaaaat
    22921 ggtgatggaa tggtttctcg gacaacaaat attaccgatt cttattggtg gaagtcagga
    22981 tatcactatc ggccaatatg caggatatga gtttctggga caagcagtta acctcatggt
    23041 tattgatgaa cgggttgatc tggatgatac tgagttgctg cctccgggaa gaacctggtt
    23101 gcaacaaata tttcgttacg atcccaaatt cctgttcaat tattctgcca tcgcatatca
    23161 gacacatttt aacagcccgg aaatgctagg tgcaatcgac gatctgcttt tcgataccat
    23221 caggctgggg cgattaaaag aaaatattca atttgcagaa cccgaagtgc gcgatgccga
    23281 tattctgagt attgatatcg gagccatacg aggtgccgat gcgccgggaa ttgctcatgc
    23341 tacgccaaat ggattatccg gatgggaaat atgtcagatc atgcggtacg ccggtatgag
    23401 cgataaaatc acatcgctgg gtatttatga atataatccc atgtttgata atcaggagtt
    23461 gactgcaaaa ctggttgcgc aaatgatttg gtattttgtt gatggctttt ataatcggaa
    23521 aagcgattac cctattgttt cggaaaaaga cttcactaaa tatatcgtca atgttgctga
    23581 aagcgattac gatctcacgt tttggaaaag catgaaaagc ggaagatggt gggttgaagt
    23641 accttttatt gtgcaacaaa gattcgagcg acatcagctt gttccttgtt catatgaaga
    23701 ttacgaaaat tcgctcaaga acgaagtgcc ggagaagtgg cttcgtatat ttcataaatt
    23761 gtcttagggg gttgggggtg agtggggcag tgggtgagtg ggtgagtggg tgagtgggtg
    23821 agtatcgtga agcgtgagtc gtgactcgtg aatgaaaata ataaggaata tagaattaag
    23881 gtcacttcct tcgaatcttc gactcttcaa tccttcgaat cttcataacc tcgtctttgc
    23941 tagccactgt attttggaat gtgtgtttaa tgtcccgcag attttcgcag agtttaatgc
    24001 ctacctcaac ctgacgctcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
    24121 tttaatgtcc cgcagatttt cgcagagttt aatgcctacc tcaacctgac gctctgccaa
    24181 ctccgtgatt ctaattgtga gtgggtgagt gggtgagttt aattcacgac tcacgattca
    24241 cgagtcacga gtcggtagtg cgttgtgagt agtgagacaa atgcagtaga atgtgcttta
    24301 tatttacact gtgcaactct ttgtccttct ttctcgcaaa gacagctaag atgcaaagac
    24361 gctaagtcca aatactttaa ctcacccact gacccactca cccactcacc attcacgact
    24421 cacgattcac gattcacgac acttactgaa ttattttctc cagcttcatg cctctggagc
    24481 ctttgagcaa aattgttgct tctctgaatt gctgcgcaag aaaccactga cgcgcctcat
    24541 cactattatg acatgcaata accggatcgc ggcgaatgtg atgaaattgc ggtccaacag
    24601 taacgatatg atcgaattta ctgtgcaatg caatatccag aatctgctta tgttcatctg
    24661 ccgaatatgt tcccagttcc agcatatcgc cgatgataat aacttttttt tgtgccgata
    24721 ttttagcgaa attctccagt gccacacgca tgcttgaagg attggcatta taggcatcga
    24781 ggatgaaata attaccattt atctcgcgat gctcactgcg attatttgca ggaatatagt
    24841 tttcgacagc ttcttgtatt tcaccataac tcacatcaaa atattttccg atgcaagcgg
    24901 ctgcaataat attatcggca ttataggctc caactaaatg actggaaatt ttaatttcat
    24961 catctataac aactgttgcc atggttgccg tttcatcaat aattcctatg gtataatctt
    25021 caggatttgt gccataacgt actgtgcgcg cataagtttt cgcaatatcg gttagcctct
    25081 cctgattgct gttccaaaaa ataacaccat cctttttaat gatgtattga tataattccg
    25141 attttccttt cgcaacacct tcaataccgc caaacccctc aagatgtgct aaaccgatat
    25201 tcgtaatcag tccgaaatca ggatctgcat atttgcagta agatgcaatt tctccctgat
    25261 ggtttgcacc catttcaatc acggcaaatt cgcaatcggc gccaatattt aaaatggtga
    25321 gtggcacacc gatatgatta tttaaattgc ctttggtagc ataggtgcga tattttttag
    25381 agagcacggc tgcaattaat tctttggttg ttgttttacc gttagaacct gtaagtgcta
    25441 aaacaggaaa ctgccatttg ctgcgataat gtgctgcgag ttcctgcatg gcaagcaacg
    25501 catcgggaac cagaataata ttagaatctg tcgcatattg cgcatcgtct acaacgcaac
    25561 aagaagcgcc tttttgcaaa gcttcgcctg cgaacatgcc gccatttact ttttcacccc
    25621 tcaatccgaa aaacatttcc cctcccgaga tcgtgcgatt atcgatggta acgccttttg
    25681 catgggagaa gcgggagtag agctcggtga tcatgcccta aaaataacaa agccctc
//
LOCUS       DAOQHY010000024        25535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_843_length_25535_cov_5.623548, whole genome shotgun sequence.
ACCESSION   DAOQHY010000024 DAOQHY010000000
VERSION     DAOQHY010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 25535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25535
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_843_length_25535_cov_5.623548"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PLL28_03465"
     CDS             complement(<1..540)
                     /locus_tag="PLL28_03465"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNF68405.1"
                     /translation="MKRLIVLPLCGLLSLIQAQVLINEAAHTNTGIVVDEDGELPDWI
                     ELYNGGVDPVNMAMWSVTDDLAIPGKWQLPDTVLAPAAHLLIMASEKNRNAYDAEMAS
                     VDHWETAVYDDDIWEYFPADAEPDPSWRTSTAPFGWDTGKGGFGFGDDDDSTIITTDV
                     RSVYYRKTFEVADKTKLIHA"
     gene            684..989
                     /locus_tag="PLL28_03470"
     CDS             684..989
                     /locus_tag="PLL28_03470"
                     /inference="COORDINATES: protein motif:HMM:NF014687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HNF68406.1"
                     /translation="MSKPFATNPELVLREELAIQRTTLANQSTFLSFIRTGLYFLLGG
                     LSLTNLLKMNDAALMEVIMFSIAALLVIIGSFNYMHHQRKIARSKLQIGQVAQATRS"
     gene            1018..1314
                     /locus_tag="PLL28_03475"
     CDS             1018..1314
                     /locus_tag="PLL28_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68407.1"
                     /translation="MPKRKLYTCSKGHQFYKSSDCRSCPTCASLAKPKDGLLSLLSAP
                     ARRALLQHGVESEHMLVNFSREEILSWHGIGLSAMAILDLQIKKLKLSYKISKL"
     gene            1348..2202
                     /locus_tag="PLL28_03480"
     CDS             1348..2202
                     /locus_tag="PLL28_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNF68408.1"
                     /translation="MNKLQFLSILLLATFSGFAQSPSARPFTIGVTEILHSNILNEDR
                     IINIYLPEGYSKDDSINYPVIYLLDGGADEDFIHIAGLVQFNNFPWIARVPNSIVVGI
                     ANTDRKRDLTYPTTITEDKQHYPTTGGSAAFMSFIEKELQPFIETNYSITHDYTLIGQ
                     SLGGLFATEVLFTKPQLFNRYIIISPSLWWDDASLLKQHPVILDSTFTQQLDIYIGVG
                     KEGLAPCTIPHVMEVDANLLSEMITYGSAPHVSVHFDYLPEEDHATVTHQAVFNAFRI
                     LYPEPKEE"
     gene            complement(2206..2787)
                     /locus_tag="PLL28_03485"
     CDS             complement(2206..2787)
                     /locus_tag="PLL28_03485"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HNF68409.1"
                     /translation="MQHNNSLNTIIRSRRSVKPEQYNTTPIAESIILEMLENARWAPT
                     HARTEPWRFVVCTGDGLKRFAEYQAEQYKLHTPAESFNANKYDKLKTTPLKASHIILV
                     GMKRQETEKLPIEEEIAAVACAVQNMLLTATANGIGAYWSTGGITYHQAMKNYMGLGE
                     NDKALGMIYIGNYDIELPEGVRSGIEDKVRWEQ"
     gene            2807..3889
                     /locus_tag="PLL28_03490"
     CDS             2807..3889
                     /locus_tag="PLL28_03490"
                     /inference="COORDINATES: protein motif:HMM:NF022818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing protein"
                     /protein_id="HNF68410.1"
                     /translation="MLRNYYIYTIGLLFSAIIFSCKGEKSGVSDDKQDPDQPTEKKVY
                     DRWLIDLEMVESGCERLDCIRSIDEPKFIPVDKVNFISDEDLVIGVRIGDEIHCYPHN
                     ILNWHEIVNDNIEGKYIAVNYCPLTGSGMAWNRNINGKLTTFGVSGLLYNSNVMPYDR
                     TTRSAWSQMKQLCVNGELADKHIESFPVIETTWSTWKKLYPNSKVVSTETGMQRDYLE
                     YPYGDYRENRDIYFEVELENDTLHPKERLYGVIQGDKAKCYRFDNFKNGIRILGDTVF
                     EKPIIVVGSERENFIIAFENPENLSFKPYTKQIPAVIQDNAGNTYDLFGTCIEGPKKG
                     TSLTAMNGFTAYWFAWYAFYPDVKFE"
     gene            complement(3895..4431)
                     /locus_tag="PLL28_03495"
     CDS             complement(3895..4431)
                     /locus_tag="PLL28_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68411.1"
                     /translation="MNKIVTIAAVAVLLSLSSCTAYLSPYTSTTQSQTNFDETQLKKV
                     QFYLSGDITLYRELAASETQITGGDIKIVDGKKVEQFVIKSGTPGVIIKAEDRDTFLV
                     SFDADGSFLRFGANNDYNGKYTLMAKEWQGRVGIVDYAGREFKSTPESIYAYLAVNME
                     KVNNTTVNTKEAGGRTIE"
     gene            complement(4483..6066)
                     /locus_tag="PLL28_03500"
     CDS             complement(4483..6066)
                     /locus_tag="PLL28_03500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNF68412.1"
                     /translation="MATSSRSPLIKALQRAFATSLYSKKHGVPLGEAHEMVQQQFSRR
                     KFITTTSTAAAITAISGSAFLEACTGKRAPVVAIVGGGIAGLYAAYILRNAGIIANIY
                     EASGRTGGRIYTMKNLMGKGTHTELGGEFIDSTHADILRLADDFNLGLIDTQVESETS
                     LKQSLFFIEGQPVDDATLLNMLAPFVPVIQQDVASLSADISYTTHTDADIALDNMSLQ
                     AYLQHIGITGPLYKLIDVAYTTEYGLDVAQQSAINFLMLIDTSMKDRFRYSGFSDERY
                     KIAEGNQSITESLTDLLEGQISLGMELIAIGQKNEGDKVLLQFSETGGKHSTVEADYV
                     ILTLPFTILRNVDITYPITANKRRAIQELGYGQNAKFFMQFQKRVWREQGYTGFMFSD
                     SIVQNAWDHTQLQPGETGGFTVFTGGAASKVMETTKMPDFAAMCMNVLSKAYPHISDP
                     NPDMLTRFHWPTHPYTKASYTCFAPGQFTTIEGAQREPAGRLYFAGEHCSFEFQGFMN
                     GAAQTGREAAEAVIKALKG"
     gene            complement(6107..7564)
                     /locus_tag="PLL28_03505"
     CDS             complement(6107..7564)
                     /locus_tag="PLL28_03505"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNF68413.1"
                     /translation="MKKALILVSFVLSLALAGAQTAYTLESIPKPFPPTSYVSNPDGV
                     LSSTVEEEINTVLRRLEDSTTAQVAVVIVNSIGSEVPGDFKTELFREWGIGQSENDNG
                     LLILLVMDQRRMEFETGYGLEGVLPDILCKHIQETYMVPHAKEGDMDNAVLSGVMQIY
                     QILTNPAYHDEIYAGSLENDANFPWWRRTTSNGLLIGFAAVYFLFAIIGYRGGMKHEK
                     SAPAYVKSQYNSKYATAKFGLLNMALPLGFYAWQELAGNLRVFEFALAAYGLLMILLM
                     EKRLRLNHFIHRDTENKLPQDTYNLLSRSHSNGWIMADIFFPIPFIFYSLINKSRMKA
                     MRNIPPLADDGTLMVKLDEKADDEFLKTYQIAEENIRSVDYDVWRKPNSNEIHIFRFE
                     NYYSKYKECPNCKSKAYLMTKNKTIVSPTYDSTGQGEKTYNCKACGHERKERYTIAKL
                     THSSSSGSGGGFGGGGGGGGFGGGSSGGGGGGSSW"
     gene            complement(7587..9167)
                     /locus_tag="PLL28_03510"
     CDS             complement(7587..9167)
                     /locus_tag="PLL28_03510"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNF68414.1"
                     /translation="MMKLHLLLPAICIIAATSTFAQSQDLGVKYANTITAEELEQHLS
                     VIASDYYEGRETGTRGLARTADYLSKQFQQIGIPPLRPIGGYYQEYPLIEFGWNASTI
                     STEKAVFNMMQDFIGYASANTSMAISDNQIIFLGYGIDDSLYSDYRGVDVKNKIVIVA
                     NGEPKLNGISRVNNKDSVTAWSKDWRKKAEAATRNGVKCLLVIEPKVPEYLNNPSWRR
                     FLEGTLMKLESEYKQPEYNNNFFITPAMAESLLGKRTKLMKKSLQSIDDTGLPRNFAI
                     HKTVVFNINKLENKIYADNVIAFVQGTDLKDQVIVVSAHYDHLGMRDSVVFNGADDDG
                     SGTVALLEIAEALAEAKKAGEGPRRSVMFVAFSGEEKGLLGSKAYAENPVVKFEYTVA
                     DLNIDMIGRVDDAHLADPNYIYIIGSDFLSSELHNINEAAAKNYSNIKLDYKYNSTTD
                     PNRYYFRSDHYNFAKNNIPCIFYFSGVHADYHQPTDDIEKIDFPLMTERARLVFHTLW
                     LLANQDARIKVDRTPPQE"
     gene            complement(9167..9607)
                     /gene="rpiB"
                     /locus_tag="PLL28_03515"
     CDS             complement(9167..9607)
                     /gene="rpiB"
                     /locus_tag="PLL28_03515"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNF68415.1"
                     /translation="MSTNIIAIGCDHAGFPYKEGIIKYLESRSYEVHDFGTHSADSVD
                     YPDHVHPVASEVETGIASFGVLICGSGNGVCMTANKHLGIRAALVWQSDIAALARQHN
                     NANVICIPARFVTLEDAIAFVDVFLHTQFEGGRHANRVTKMTDF"
     gene            complement(9677..10507)
                     /gene="tatC"
                     /locus_tag="PLL28_03520"
     CDS             complement(9677..10507)
                     /gene="tatC"
                     /locus_tag="PLL28_03520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNF68416.1"
                     /translation="MAAIKKNPDEMSFMEHLEALRWHIVRIAVVIFLFSIAFLIYPQF
                     IFDKIILAPKSPDFITYRIFCNIGERWNIDGICVDNLDYDLFYLNMTSGFLFYIKIAF
                     MAGIIFAVPYILWEIWRFVRPALHDKEVSNLGGIIIIATLLFYVGALFGYFLMAPFSI
                     NFLATFSLSDQIQNQFTFDSYLGILTGMVLWTGVIFEIPMVAYFLAKLGLLGREFLAK
                     NRKYAAVISLILAAVITPSSDAFSLFLVALPLWGLYEVSIVVVNVVEKRRRKRELAGN
                     "
     gene            complement(10526..11524)
                     /locus_tag="PLL28_03525"
     CDS             complement(10526..11524)
                     /locus_tag="PLL28_03525"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF68417.1"
                     /translation="MKKLSIIALSALAFSACVPQRQYQDAITARDACMQDTAVLHSQL
                     RMKTKEVDELSQQLDEVYASINLMRKDSIDIHNRYDAMVKANEQLREIEDVLNNRINQ
                     LLALSANENQQLREDLQKKSDELIAKEKELNAKAMTLSDQQKSIDALTADLEARSKRV
                     EELESILNKQEQTVTDLKNTIDKALSGFSASDLTVERKNGKVYVSLSEKLLFASGSTV
                     VDPKGKDALKKLADVLKANPDIRVEIEGHTDNVPLKSNNFPKDNWDLSVLRATSIVKI
                     LTQENGVDAKRVIAEGRGEFFPIADNTTTEGKARNRRTEIILSPNLDALYDLINSK"
     gene            complement(11584..13128)
                     /locus_tag="PLL28_03530"
     CDS             complement(11584..13128)
                     /locus_tag="PLL28_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HNF68418.1"
                     /translation="MDRNEQFKQVIQEGYNFQGTSIVLGTAILDRQPMPQTHVKVPLR
                     MMNRHGLVAGATGTGKTKTLQGIVEQLSDNGVGVLIMDIKGDVSGIAMPGTGNPKIDE
                     RMQQIGKSWQAKGYPVELMSISNENGVRLRATVSEFGPVLFSKILELNDIQEGVVSLV
                     FKYCDDNKIPLLDLKDFRKTLNYLTSEGKKDISADYGTISTSSAGTILRKVIEIEEQG
                     ADLFFGEKSFDVEDLCRMNDRGQAYVNILRLTDIQSKPKLFSTFMLCLLAEVYNTFPE
                     IGDKEDPKLVIIIDEAHLIFKEASKSLLEQIQTIVKLIRSKGVGIFFCTQLPTDIPAE
                     VLSQLGLKVQHALRAFTANDRKAIKLTAENYPITDFYQTEDLLTNLGIGEAIITALNE
                     KGAPAPLAHTLLVAPQSRMDVLSAEEMQQLNNISSLTKKYNESVDRESAYEILNKKLA
                     ASEQAAEREKQLKEEEAKQKLEERERKVKKTKEKPLIDKTTQHQITRTVINVLERGLL
                     GMLKRR"
     gene            13184..14557
                     /gene="radA"
                     /locus_tag="PLL28_03535"
     CDS             13184..14557
                     /gene="radA"
                     /locus_tag="PLL28_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNF68419.1"
                     /translation="MANKAKTAFFCRNCGFESSKWLGKCPSCNEWNTFTEEVIERPKD
                     AASWKDSKPEKRISGPVQLSEITASDTGRLVTGDLELNRTLGGGIVAGSLVLIGGEPG
                     IGKSTLLLQLALELKQIKVLYVSGEESEQQIKMRADRLKGNNQGLYISTEVAISKLFM
                     QIKQLQPEMVVIDSIQTLVSDFVDATAGSIAQIKECAGQLQRFAKETNVPVFIIGHIN
                     KEGYIAGPKILEHMVDTVLQFEGDRNYTHRILRTIKNRFGSTSEIGIYKMEGDGLQQV
                     NNPSELLITEKEEPLSGVAVAATMEGMRPMLIEVQALVSPAVYGNPQRTATGFDLRRL
                     NMLLAVLEKRGGFAYGNKDVFLNIAGGLRVEDPAIDLSIACALLSSLTDMALPLNMCF
                     AAEIGLSGELRGVARIEQRITEAEKLGFDRIYIAKHNMKSLKQNKYNIELRAAGKLEE
                     VYGQLFV"
     gene            14700..15398
                     /locus_tag="PLL28_03540"
     CDS             14700..15398
                     /locus_tag="PLL28_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNF68420.1"
                     /translation="MFPLLLLNELSDMFYPRLCTACKEPLLAHEHIICLRCRIELPRT
                     DHGLFHDNPVAVKFWGKVPIEEAAAMLHFHKGSRVQSLMHALKYKNRTDVGITLGNML
                     GYYCKQYHLFKQITAITAVPLHKERLAVRGYNQSAVIGEGLAEVLQVPFKGEWLERIT
                     PTATQTKKSRLARWDNVKDVFRVTDPDGIAHQHILLIDDVITTGSTLEACALKLIQSV
                     GVKVSIAAIAFADR"
     gene            15775..16737
                     /locus_tag="PLL28_03545"
     CDS             15775..16737
                     /locus_tag="PLL28_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNF68421.1"
                     /translation="MNKTILFDFNVDKEARTIRVDRSFDAPRDLVWAAWTESDILDQW
                     WAPKPYRNVTLSMNFVEGGRWHYYMISPKDEKHYCLFDYHRIIPETMYSGMDAFCDID
                     GNISESMPRMQWENSFEKQGDTTVVHVLINFNKIEDLEAVISMGFKEGFAMGLENLDA
                     YIASQFYLRKSKKTSNAARVCTYLNFPGNTEDAMLFYKGIFKTEFVQGIQRFGDVPSD
                     AGAPPVPDALKQMVLHVELPLIGGHILMATDAPQEMGFEVKVGNNMHISLEPDSKDET
                     TRIFNALAEGGNITMELQDMFWGAYYGSCTDKYGINWMLNFQQH"
     gene            complement(16792..17982)
                     /gene="hflX"
                     /locus_tag="PLL28_03550"
     CDS             complement(16792..17982)
                     /gene="hflX"
                     /locus_tag="PLL28_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNF68422.1"
                     /translation="MAHKPTIKPNETETCILVGLVTKDQTLEQLNEFLDELEFLAETA
                     GAVTKAKFYQKLPHPDTKSYVGKGKLFEIMDYVVANEIDIVIFDDEISPSQLRNIEET
                     LKVKVLDRSMLILDIFATRAQTVQAKTQVELAQTQYMLPRLKGLWGHLDRIKGGIGMR
                     GAGEKEIETDRRVAQQRIALLKQQLEKIGLQNETQRKNRGELIRVALVGYTNVGKSTL
                     MNLLSKSEVFAENKLFATLDTTVRKVVLDRTPFLLSDTVGFIRKLPHHLIESFKSTLD
                     EVRESDILLHVVDVSHPAFEEHIHVVNETLKELEAIDKPTILIFNKMDAYEERYFDKY
                     LTPDVKRDILSELENTWMQRLNAQCVFISAINRTNIELLRSMLTDMVKEQYQIRYPYK
                     TEFF"
     gene            18099..18986
                     /locus_tag="PLL28_03555"
     CDS             18099..18986
                     /locus_tag="PLL28_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68423.1"
                     /translation="MQINFLRHNEIDRKNWDRCIDNSLNALPYAYSWYLDIVSNGQWD
                     AIIIDDYAAVFPIPFKNRLIYKQVYQPFFTQQLGLFYTDKLYHTKLPDVLKALPASYR
                     KIHLHLNIENDLGEVFSAVEKRVTHHIDLNKSYQSIASDYNEQIRRNLKKSLTHHLQV
                     ETSSDVNILISLREMFLQEALKGKQSAADTDRLKKLIQAILDNKKGEIKFVRGAENDI
                     QAAVCLLQSNGYIIYLSSVSSDVGKANHAMTFLIDDMLKSNANTQQTFDFEGSMIPGI
                     ARYFKGFGGKEKWFSVIQK"
     gene            complement(18999..20273)
                     /gene="glyA"
                     /locus_tag="PLL28_03560"
     CDS             complement(18999..20273)
                     /gene="glyA"
                     /locus_tag="PLL28_03560"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNF68424.1"
                     /translation="MRRDTEVFDIIKKELHRQTEGIELIASENYVSDQVLEAMGSCMT
                     NKYAEGYPGKRYYGGCEYVDVTENIAIDRAKKLFGAEYANVQPHSGAQANAAVMLACL
                     NPGDTLLGFNLSHGGHLTHGSPVNFSGKLYRPVFYSVEEETGRIDMDKVEATAIAEKP
                     KLIICGASAYARDWDYARFRAIADKVGAILLADISHPAGLIAAKLLNDPMPHCHIVTT
                     TTHKTLRGPRGGMIMIGKDFPNPWGQTTPKGEVKMMSAVIDSAVFPGTQGGPLEHVIA
                     AKAVAFGEDLDEGYVAYCKQVIANAQAMANAFVSKGYKIISGGTDNHLMLIDLRNKNI
                     SGKKAEGLLVDAHITVNKNMVPFDDKSPFVTSGIRIGTPAVTTRGMKENDMVRIVEWI
                     DRLIMAPDDDAVKTAVRNEVNAFASQFPLYAY"
     gene            complement(20598..23366)
                     /locus_tag="PLL28_03565"
     CDS             complement(20598..23366)
                     /locus_tag="PLL28_03565"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNF68425.1"
                     /translation="MLKKLLLSGSVFGLTILGLSGQVVINEYSAANYSDIADNYGEYE
                     DWIELYNAGATAVDLSGYHLSDRIGSPTKYTIPPGVNISAGGYLRFWCSNRNTAVGTN
                     YHTNFKLTQTTGAEDVVFADAAGTVLDTRELNNPNQTNHSWGRYPNGGADWKIFTDPT
                     PNASNSTTPKTAYAHKPDMEPNSGNFAGSVTVTITAPEPGMTIRYTTNGNLPTAASTA
                     YTGPITITETTVLRTVAFAASADTLASFCSTNTYFIDETTTMPVISVAGDQVDDLLNA
                     TGWGIEPVGSFELFDKDFNLIDEAVGDFNEHGNDSWAYDQRGFDFIVRDQFGYDNDID
                     QTFFNDVTDREGYQRLIVKAAANDNYPSSSGGAHVRDAFVHHISGLDGLELDERSTFF
                     NIMFLNGDYWGVYDTREKADDADYTDYYYGQGEYEMDYIKCWGGTWAEYGTMAPWSPF
                     VTFVTTNDMTIPANYDYVKDNFNTMSLIDYVLTNTFSVCTDWLNWNTAWWRGYDATGS
                     NLTWRYALWDEDATFGHYINYTGVPDDSPTASPCDPLTLGGVDPNGHIDMLNSLLDNP
                     DFHSDYINRFADLINTTFSCEVLLPRIDSMRAVLDPEMTRHTDKWGGTYTGWSNNYDE
                     LHDFIAERCAYLPSGVEDCFDVTAYGIVVKIEPAGSPNDVRVSTIIPDTYPYSATYFS
                     GTTLGFQAIADPAFTFDHWEFINHVPSPSTTDDSVTVSLTTTDTIIAWFAGSELPMYD
                     FTLDIDPPGAGVATVEGFSPAAYPYAGAYESGTFIDLSATPTGSYVLDYWSLENHTAN
                     PDPFAPDIFFALSDIENAVAHFRLKTSVEENGVSVSMTAVPSVTAGPVQVHYGLSAAQ
                     DNMQLTIYSLTGQLIADLGADQLISGAQGEHIVSVDLNKYHLANGMYFIRLTAGNQSI
                     NERVMLAK"
     gene            23558..24562
                     /locus_tag="PLL28_03570"
     CDS             23558..24562
                     /locus_tag="PLL28_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNF68426.1"
                     /translation="MKVIIPVAGVGTRLRPHTYSQPKPLIPVAGKPILGFIIDRMVKA
                     GFTEFIFVIGYLGEKIESYVLEHYPELKCRFVIQNKREGLGHAIFLTRPLISENEELY
                     IVLGDTIFEADIEAVMTMPESALGLKRVDDPRDFGVAEVEDDMRITRVIEKPSIPKSN
                     LALVGLYKIKESAQLFNALEQIMAHGYKTQGEYHLTDALMMMIKDGITFRGFKITNWY
                     DCGKKEILLETNAILLKKLHPQGQHQHAYPNTILIEPVSIAPGCDIRDSIIGPNVTLG
                     ENTSVHYSILRHSIIGNDATIDDVVLLDSVIGSDTVIKGMSQSLNIGDNTEIDLSKSG
                     "
     gene            24834..>25535
                     /locus_tag="PLL28_03575"
     CDS             24834..>25535
                     /locus_tag="PLL28_03575"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNF68427.1"
                     /translation="MHNRITALFGIEYPIIQAGMIWCSGWKLASAVSNAGGLGLIGSG
                     SMYPDVLREHIQKCKAATNKPFGVNVPLLYPDIDKHIQIIIEEGVKIVFTSAGNPKTW
                     TSMLKQHGIKVVHVVSSRKFALKCQEAGCDAVVAEGFEAGGHNGREETTTLCLIPSVA
                     DAVTIPVIAAGGIGDGRAMLACMVLGAEGVQIGTRFIASIESSAHDAFKQTVIHADEG
                     STILTLKEITPVRMVR"
BASE COUNT         7374 a         5391 c         5293 g         7477 t
ORIGIN      
        1 ggcatgaatc aattttgttt tatcggcaac ttcaaatgtt tttctataat aaaccgatct
       61 cacatcagtg gtaataatgg tactatcatc atcatcccca aaaccaaatc caccttttcc
      121 ggtatcccaa ccaaagggtg cagttgaggt acgccaagat ggatcgggct cagcatctgc
      181 agggaagtat tcccatatat catcatcata cacagcagtc tcccaatgat cgacagaggc
      241 catttctgca tcgtaggcat tgcgattttt ttcagacgcc atgatcagca gatgcgccgc
      301 cggtgcgaga acggtatcgg gaagctgcca ttttccgggt atggctaaat catcggtaac
      361 cgaccacatg gccatgttca ccggatcaac accaccattg tataattcaa tccaatcagg
      421 caattcgcca tcttcatcta ccacaatacc ggtgttggta tgcgcagctt cgttaattaa
      481 tacttgtgcc tgaataagcg acaaaagccc acaaagtggc aatacgataa gtcgtttcat
      541 ttggtgtgcc ctgggttgca attaggttag ggtaatcaaa tatagaagta ttgcggcggt
      601 aatccaaact ttaacaattc ggacatcatt cgaattattt caatatctat tatgttaatt
      661 tgtagaatca accacaatcc gctatgtcaa aaccattcgc cacaaatccg gagttggtgc
      721 ttcgtgaaga acttgccata caacgaacaa cccttgcaaa ccaatcaact tttctttctt
      781 ttataagaac cggattgtat tttttgttag gtggattgag ccttacaaat ttgttaaaaa
      841 tgaatgacgc agcactgatg gaagtaatca tgttttcaat tgctgcctta ttagtcatta
      901 ttggaagctt taattatatg catcatcaac gcaaaattgc cagaagtaaa ttgcaaatag
      961 gtcaagtagc gcaagcaaca aggagttaaa atatagatat ttattatctt tgaaattatg
     1021 ccaaagcgca aattatacac ttgctctaag ggtcatcaat tttacaagag cagcgattgt
     1081 cgctcgtgtc ctacatgcgc atcacttgcc aagccaaagg atggattatt atctttatta
     1141 tctgcaccgg caagaagagc attattacaa cacggagttg aaagtgagca tatgcttgtc
     1201 aatttttcca gagaagaaat attaagttgg catggcattg gtctttctgc gatggcgata
     1261 ttagaccttc aaataaaaaa gttgaaattg tcttacaaaa tttcaaagct ctaaaattat
     1321 ttttatcgaa ttgccttgct tatgcatatg aataaattac aattcctgtc aatacttctt
     1381 cttgcaacat tttccggatt cgcacaatct ccttccgccc ggccatttac aattggcgtt
     1441 actgaaatcc tccattctaa tattttaaat gaggatcgta tcattaatat ttatcttccc
     1501 gaaggatata gcaaagatga ctctattaat taccctgtaa tttatttact ggatggcggt
     1561 gcagatgaag atttcataca tatagccgga cttgtccaat tcaacaattt tccatggata
     1621 gcacgcgtcc ctaactcaat tgttgttggc attgccaata ccgaccgcaa acgcgatctc
     1681 acctacccta ccaccataac tgaggacaaa caacattacc caacaacagg cggctctgct
     1741 gcattcatgt cattcattga aaaggaatta caacctttca tcgaaacgaa ttattccatc
     1801 acccatgact acacactcat aggacaatcg ctaggaggtt tattcgcgac agaagtgctg
     1861 ttcacaaaac cgcaattgtt caacaggtat atcataataa gtccgagctt gtggtgggat
     1921 gatgcttcat tgttgaaaca acatcctgtc attctcgaca gcacctttac tcaacaattg
     1981 gatatttata tcggtgtagg aaaagaaggg cttgcaccgt gtactattcc gcacgtaatg
     2041 gaggttgatg ctaacttatt atcggagatg atcacttacg gatcagcacc gcatgtatca
     2101 gttcattttg attacttgcc ggaagaggat catgcaacgg tgacgcacca ggcagtgttt
     2161 aatgcattca gaatattata tccggaacct aaggaagaat aaatgttatt gctcccagcg
     2221 caccttgtct tctattccgc tgcgcacgcc ttccggtaat tctatatcgt aattgccgat
     2281 ataaatcata ccgagcgctt tatcattctc tcctaaaccc atgtagtttt tcatagcctg
     2341 atgataggta attccaccgg tactccaata tgcacctatg ccattagccg ttgctgtcag
     2401 cagcatattt tgcacggcac aagctacagc ggcaatctct tcttctatcg gcaatttctc
     2461 agtttcctgg cgcttcatgc caaccaaaat aatatgcgat gcctttaatg gtgtagtttt
     2521 aagtttgtca tacttattag cattaaacga ttccgccggg gtatgcaact tatattgctc
     2581 agcctggtat tcggcaaaac gcttaagccc gtctcccgtg caaaccacga aacgccaagg
     2641 ctcagtgcgg gcatgcgttg gcgcccagcg tgcattttcc agcatctcta aaatgatgga
     2701 ctccgcgata ggagtagtgt tatactgttc aggtttcaca gaacggcgac tgcgaatgat
     2761 agtatttaac gagttgttgt gttgcatctt gtaactttgt ggtctgatgt tgcgaaatta
     2821 ttacatatac accataggcc tgcttttctc tgctataata ttctcctgca aaggagaaaa
     2881 atccggcgtt tccgatgata agcaggatcc cgatcaacct acagagaaaa aagtatacga
     2941 caggtggctg attgatttgg aaatggtgga atcaggatgc gagcgtttgg attgtattcg
     3001 gtctatcgac gaaccaaaat ttatacctgt agataaagtt aattttatca gtgatgaaga
     3061 cctggtgatt ggcgtgcgca ttggcgatga gatacattgc tatccgcaca atattctcaa
     3121 ctggcacgag atcgtaaatg ataatataga aggaaaatat atcgccgtga attattgtcc
     3181 gctcaccggc tccggtatgg cgtggaatag aaatatcaat ggcaaactga caacctttgg
     3241 tgtatccgga ttattataca atagcaatgt aatgccttac gatcgcacca cgcgttctgc
     3301 atggagtcag atgaaacagt tatgtgtgaa cggcgagctt gccgataaac atatcgaatc
     3361 attcccggtg atagaaacca cctggtcaac gtggaaaaaa ctgtatccca acagcaaagt
     3421 ggtatcaacc gagacaggta tgcagcgcga ctatctggaa tatccctatg gcgactatcg
     3481 tgaaaacaga gacatttatt ttgaggtgga attagaaaac gacaccttac atccaaagga
     3541 aagattgtat ggagtaattc aaggtgataa agctaaatgc tatcgctttg ataatttcaa
     3601 aaacggcatc aggattttgg gagataccgt atttgaaaaa ccgatcattg tagttggcag
     3661 cgagcgcgaa aactttatta ttgcatttga aaatccggaa aatctaagtt tcaagcctta
     3721 taccaaacag atccctgctg ttatacaaga taatgcaggc aacacctatg atctattcgg
     3781 cacttgtata gaaggaccta aaaaaggcac atcattaact gcaatgaatg gatttacggc
     3841 atactggttt gcctggtatg cgttttatcc tgatgttaaa tttgaataag gaaattattc
     3901 gatggttctg ccaccggctt ctttagtatt caccgttgtg ttgttcacct tttccatatt
     3961 cacagccagg taagcataaa tactttcagg agtacttttg aattctctgc cggcataatc
     4021 tacaatacct acacgacctt gccattcctt agccatcaag gtgtattttc cattataatc
     4081 gttgttggca ccaaagcgca ggaaactgcc atcggcatca aagctcacca gaaaagtatc
     4141 tctgtcttca gctttaatta taacaccggg agttcctgat ttaatgacaa attgttctac
     4201 tttttttcca tcaacgatct taatatcgcc ccctgtgatt tgtgtttcac tggcggctaa
     4261 ctcgcggtac aaagtaatat cgcccgacaa ataaaactgc acttttttca attgcgtttc
     4321 gtcgaaattt gtctgcgact gtgttgttga ggtatatggg ctaagatatg ctgtgcatga
     4381 agacaatgac agtaaaacgg ctactgccgc gatggtaaca atcttattca tccggtggtg
     4441 tttggctgtt aaattgcaaa atttatgcca tttcggtcaa aatcaacctt ttagggcttt
     4501 tatcactgct tcggcagcct ctctgcctgt ttgagcagcg ccgttcatga aaccctgaaa
     4561 ctcaaagctg cagtgctctc cggcgaaata caatcgccct gccggctcac gttgtgcacc
     4621 ttcaatagtg gtgaactgac cgggagcgaa acaagtgtaa ctggcttttg tgtaagggtg
     4681 ggttggccag tgaaagcgcg tcaacatatc aggatttggg tcgctaatgt gcggataagc
     4741 tttcgataat acattcatgc acatggcggc aaaatcgggc atttttgtgg tttccattac
     4801 tttgcttgca gcgccacctg tgaatacggt aaatccgcct gtttcaccgg gttgtaattg
     4861 tgtatgatcc catgcgtttt gcactatcga atcgctgaac ataaatccgg tatatccttg
     4921 ttcccgccat acccgctttt gaaactgcat gaaaaatttc gcattctgtc cgtatccaag
     4981 ctcctgaatt gcacgccttt tatttgctgt tatcggataa gtgatatcga cattacgcaa
     5041 tatggtaaac ggcaaggtta aaataacata atcagcttca acggtgctgt gtttccctcc
     5101 ggtttcggaa aattgaagca acactttatc gccttcattt ttctgcccga tagcaatcag
     5161 ttccatgcca agtgaaattt ggccttccag cagatctgtc aaactttcag ttatgctttg
     5221 attaccttca gcaattttat atcgctcgtc gctaaatccg gaatagcgga atctatcctt
     5281 catagatgta tcgatcaaca ttaaaaaatt gattgccgat tgttgagcga catcaagtcc
     5341 gtattcagtg gtatatgcta catctatcaa cttatataat ggcccggtta tacctatatg
     5401 ctgaagataa gcctgcagcg acatgttatc taatgcaata tcagcatcgg tatgtgtggt
     5461 atatgaaatg tcggcactca atgatgcaac atcctgttga attaccggaa caaaaggtgc
     5521 aagcatattc aataaagttg catcatcaac cggttgtcct tcaataaaaa ataaactctg
     5581 tttaagtgaa gtttccgatt caacttgagt atcaatcaat ccaagattaa aatcatctgc
     5641 caggcgcaaa atatcggcat gcgtgctatc aataaattca cctccaagtt ctgtatgtgt
     5701 tccttttccc atgagatttt tcatggtgta aatccggcct cctgttcttc ctgaggcttc
     5761 gtaaatattt gctattattc cggcattgcg caaaatatat gcggcatata agccagcaat
     5821 accacctcca acaatcgcca caaccggtgc gcgttttccc gtacaagctt ccagaaatgc
     5881 tgacccgctg attgcagtaa ttgcggctgc tgtagaggta gtggtaataa attttcttct
     5941 gctgaattgt tgctgcacca tctcatgcgc ttcgcccaaa ggaacaccat gttttttact
     6001 gtacaaggat gttgcaaaag cacgttgtag tgcctttatc aggggcgatc gcgaagatgt
     6061 tgccataaaa tatgtaatta atgaattgaa tttagattgt atgtggtcac cagctgctac
     6121 caccgccgcc gccaccggag cttccaccac caaagccgcc accaccgcca ccgccaccga
     6181 atccgccacc ggagccgctg ctgctggagt gtgtgagttt agcaatggta tatcgctcct
     6241 tgcgttcgtg cccacatgcc ttgcaattgt aggttttttc tccttgaccc gtactgtcgt
     6301 atgtaggtga aacgatagtt ttatttttag tcatcagata agccttactc ttacaattcg
     6361 ggcattcttt atacttgctg taataatttt caaatcggaa aatatgaatt tcatttgagt
     6421 ttggttttcg ccaaacatcg taatctactg aacgaatatt ttcttctgca atttgatatg
     6481 ttttcaagaa ttcatcgtca gctttttcat ctaatttcac catcaaggtg ccatcatcag
     6541 ctagcggcgg aatgtttcgc attgccttca tgcgactttt attgataagg gaataaaaaa
     6601 taaatggaat tggaaaaaat atatccgcca ttatccaacc attgctatgt gagcgcgaga
     6661 ggagattata ggtatcctgt ggtaatttat tttcagtatc tctatggata aaatgattta
     6721 atcgcaaacg tttttccatc aacaaaatca ttaataatcc atatgctgcg agagcaaatt
     6781 caaacacacg taagtttccc gccaattctt gccaggcata gaatccaaga ggcaaagcca
     6841 tattcagcaa accaaatttc gcagtggcat atttgctgtt gtattgactt tttacataag
     6901 ccggagctga tttttcgtgc ttcataccac cgcgataacc aatgatggca aacaaaaaat
     6961 aaactgctgc aaatccaata agcagtccat tactagttgt gcgacgccac cacgggaagt
     7021 tggcatcatt ttcaagtgaa ccggcataaa tttcatcatg ataagccgga ttagtgagta
     7081 tctgataaat ctgcatcaca cccgaaagca ctgcattatc catatcaccc tcctttgcat
     7141 gaggcaccat atatgtttcc tgaatatgct tacaaaggat atcaggcaat accccttcga
     7201 gtccatagcc cgtttcgaac tccatgcgtc gttgatccat taccagcaat atcagtaagc
     7261 cattgtcgtt ttcggactga cctatacccc actcgcggaa taattcggtc ttaaaatcac
     7321 cgggcacttc gctgccaata gaatttacaa tgacaacggc cacctgagca gtagtggaat
     7381 cttcgagtct acgcaaaaca gtattgatct cctcttctac agttgaactc agcacaccat
     7441 caggattact tacgtatgat gttggcggga atggcttggg gatcgactca agggtatagg
     7501 cagtttgcgc gccggctaaa gcaagcgaca aaacaaaaga aaccagtatc agggcttttt
     7561 tcatctgccc taaatatcgg aaatttttat tcctgcggag gggtcctatc cacttttatg
     7621 cgggcatcct gattggcaag caaccacagg gtatggaaaa ccagtcgcgc cctttctgtc
     7681 atcaaaggga aatcaatctt ttcaatgtcg tcggtaggtt gatgataatc ggcgtgaacg
     7741 ccactgaaat agaaaatgca agggatatta ttttttgcga aattataatg atcgctacgg
     7801 aagtaatatc tgtttggatc ggtagtggaa ttgtatttgt aatcgagttt gatattgcta
     7861 taattctttg ctgctgcttc gttgatatta tgcagctcag aactaagaaa atcggaacct
     7921 ataatatata tataattcgg gtcggcaagg tgtgcatcat caacgcgacc gatcatatca
     7981 atattcaggt cggctacggt atattcaaat tttaccacag gattttcggc ataggctttt
     8041 gaccccaaga gtcccttctc ctcccctgag aaagcaacaa acattaccga acgacgtgga
     8101 ccctcgcctg cttttttagc ttcagcaagc gcttctgcaa tttcgagcaa tgctacagtt
     8161 cctgagccat cgtcatctgc gccattaaat acaacagagt cgcgcatgcc aaggtgatcg
     8221 taatgtgccg aaaccactat cacctgatct tttaaatcgg tgccctgaac aaatgcaatt
     8281 acattatcag catagatttt attctcgagc ttattaatat taaaaaccac tgttttatgg
     8341 atggcaaaat ttctcggcaa tccggtatca tcaattgatt gcagcgattt cttcatgagt
     8401 ttagttcgct tgccgagaag gctttcagcc atagcgggtg tgatgaaaaa attattgttg
     8461 tattccggct gtttatattc actttcaagt ttcatgagcg tgccttcgag aaaacgtcgc
     8521 catgaagggt tatttaaata ttcaggtact ttcggttcta tcaccagtaa gcatttcact
     8581 ccgttacgcg tggcggcctc agcttttttt cgccaatctt tagaccaggc agtaacgcta
     8641 tctttattat ttacgcgtga aattccattt aattttggtt cgccatttgc aacgatgaca
     8701 attttatttt ttacgtctac cccgcgataa tcgctgtaaa gtgaatcatc aatgccataa
     8761 cctaaaaaaa tgatttgatt atcagaaatt gccattgaag tatttgccga tgcataacca
     8821 ataaaatcct gcatcatgtt aaatactgct ttttctgtgc tgatggtaga agcattccaa
     8881 ccgaattcta ttaatggata ttcctgataa taaccaccga tagggcgcag tggcggtatg
     8941 ccgatttgct gaaattgttt cgacagataa tctgctgttc ttgcgagtcc gcgcgtgcct
     9001 gtttcgcgac cttcataata atcagaggca attacggaaa ggtgctgctc gagttcttcg
     9061 gcagtaatgg tgtttgcata ttttacaccg agatcttgcg attgagcaaa agttgaagta
     9121 gctgcgataa tacaaattgc cggcagcagt aaatgcaatt tcatcattag aaatcagtca
     9181 ttttggtaac gcggttggca tgtcggccac cttcaaattg tgtgtgcaga aaaacatcaa
     9241 caaaggcgat ggcatcttcc agagttacaa atcgggcagg aatacatatc acatttgcat
     9301 tattatgttg acgggcgagt gccgcaatat ctgattgcca aactaatgct gcacgaatgc
     9361 caaggtgttt attagcagtc atacaaaccc catttccact gccacaaatc aacactccaa
     9421 aagaagcaat acctgtttca acttcagaag ctacaggatg cacatgatcc ggataatcga
     9481 ctgaatcggc agaatgcgta ccgaagtcat gtacttcata acttctgctt tcgagatatt
     9541 ttatgatacc ctctttataa ggaaatccgg cgtgatcgca tccgatggct atgatgtttg
     9601 tagacattct acaaagatag aacctttacc caatcagtcg gaaggtgtaa taatgttaaa
     9661 aaggactaaa atggcgtcaa tttcctgcca attcgcgttt gcggcggcgt ttttcaacca
     9721 cattcactac aacgatactc acttcataga gaccccacaa tggtagagcc accagaaata
     9781 agctaaaagc atcgcttgaa ggggtgatca ctgcggcaag aataagagaa attacggcag
     9841 catattttct gttctttgca agaaattctc ttccgagcaa tcccagcttc gccaaaaaat
     9901 aagcaaccat tggaatttcg aatattacac ctgtccacag taccataccg gtaagaatac
     9961 caagataaga atcgaatgta aattgatttt gtatttgatc gctcaatgaa aatgttgcga
    10021 ggaaattaat agaaaacgga gccatcagaa aataaccgaa gagagcaccc acataaaaca
    10081 gcagtgttgc aataattatt attcctccaa ggttggaaac ttctttatcg tgcaaggcag
    10141 ggcgaacgaa acgccagatc tcccataaaa tgtatggcac ggcaaatatg attcctgcca
    10201 taaatgcaat ttttatataa aacaggaatc ccgaggtcat gttgagataa aacaaatcat
    10261 aatcgagatt atctacgcaa ataccgtcga tattccatct ttcaccgata ttacaaaata
    10321 tgcggtaagt gataaaatca gggctcttag gagcgagaat gattttatcg aaaataaatt
    10381 gcgggtagat cagaaatgca attgaaaaca aaaagatcac cacagctatc cggacgatat
    10441 gccaacgcag tgcttcgaga tgctccatga atgacatctc atccggattc ttctttatcg
    10501 cagccatact aaataaaaac ccgaattatt tactgttaat aagatcatag agggcatcca
    10561 ggttcgggct gaggatgatc tctgtgcggc gattgcgcgc cttaccttcg gttgtggtgt
    10621 tatcggcaat agggaaaaat tcaccgcgac cttcagcgat cacgcgttta gcgtcgacac
    10681 cattttcctg agtcagaatt tttacgatac ttgttgcacg caatacacta agatcccagt
    10741 tgtcctttgg aaaattattg ctttttagcg gtacattatc ggtatgacct tcaatctcaa
    10801 ctctgatatc gggatttgcc ttcaacacat cagcaagttt tttcaaagca tctttccctt
    10861 tgggatccac aacggtactt cctgatgcga ataataattt ctcgctgagt gatacataca
    10921 cttttccatt tttgcgctca acggtaagat cacttgcact gaatcctgaa agggccttat
    10981 cgattgtatt tttaagatca gtaactgttt gttcctgttt atttagaata gattcaagtt
    11041 cctctacacg cttgctgcgc gcttcaagat ctgcagtcaa tgcatcaata cttttttgct
    11101 gatctgaaag cgtcatcgcc ttagcattca attctttttc tttagctatc agttcatcag
    11161 actttttctg aaggtcttca cgcaattgct gattttcgtt tgcagataat gccaacaatt
    11221 ggttaatgcg attgttgagc acatcttcaa tttcacgcaa ttgctcattc gccttcacca
    11281 tagcatcata gcgattgtgt atatctatgg aatctttgcg catcagatta atagatgcat
    11341 acacctcatc aagttgctgg gaaagttcat cgacttcttt tgttttcata cgcaattgac
    11401 tgtgcaacac tgctgtgtcc tgcatgcagg catcgcgagc ggtaatggca tcctgatatt
    11461 gacgctgcgg cacacaagcc gaaaaagcca atgctgataa tgctataatg ctgagttttt
    11521 tcatattctt catgtttaac tgggcgaaat aaacggtttt ttattattgt atcgtattcg
    11581 ttatcaacgg cgtttgagca taccgagcaa tccacgctcc aatacgttaa tcaccgtacg
    11641 ggtgatctga tgctgtgtgg tcttgtcgat caatggtttt tctttggtct ttttcacttt
    11701 gcgctcgcgt tcttcgagtt tctgtttggc ttcttcctcc ttcaattgct tttcccgctc
    11761 agccgcttgt tcacttgcgg ccagtttttt atttaatatt tcataggcac tctctctgtc
    11821 gacagattca ttatattttt ttgttaacga tgaaatatta ttcaactgct gcatttcttc
    11881 tgctgaaaga acatccatac gcgactgagg tgccaccagc aaggtatgtg ctaaaggagc
    11941 gggtgcacct ttttcgttga gtgccgtaat gatggcttca ccgataccga gatttgtaag
    12001 gagatcttct gtttgataaa aatcggtaat aggataattt tctgctgtca gtttgattgc
    12061 ttttctatca tttgccgtaa atgcgcgcaa ggcatgttgc accttcaaac ccagttgcga
    12121 caatacttct gcggggatat cggttggcaa ttgggtgcag aagaaaatac ctaccccttt
    12181 tgagcgaatt aattttacaa tagtttgaat ctgctccaat aagcttttgc ttgcttcctt
    12241 aaaaataaga tgtgcctcat ctatgatgat caccagtttc ggatcttctt tatcgccaat
    12301 ttcggggaat gtattatata cctcagccag caaacacaac ataaatgttg agaataactt
    12361 cggcttactt tgaatatctg tgagtcgcaa gatgtttacg tatgcctgac ctctatcatt
    12421 catgcgacag agatcttcca catcaaaact tttttctccg aagaaaagat cagcaccttg
    12481 ttcttcgatc tctattactt ttcgcaaaat ggtgccggcg cttgatgtgc tgatggttcc
    12541 gtaatctgcg gagatatctt ttttcccttc ggaagtaagg taattgagtg ttttacggaa
    12601 atctttcaga tccaggagtg gaattttgtt atcatcgcaa tatttgaata ccagcgatac
    12661 aacgccttcc tgaatatcat tgagttcaag gatcttagaa aacaatacag gaccaaattc
    12721 cgaaactgta gcacgaagtc gaacaccatt ttcattgctg atgctcatca attcaacagg
    12781 atatcctttt gcctgccagc tttttccaat ttgctgcatg cgttcatcaa ttttcggatt
    12841 tcctgttccg ggcatagcaa ttccactcac atcgcctttg atatccatga tgagcacacc
    12901 aacaccatta tcacttaatt gctctactat gccttgcagt gtttttgttt ttccggttcc
    12961 ggtagcgcct gccaccagtc catggcgatt catcatcctc aatggcactt ttacatgggt
    13021 ttgaggcata ggctggcggt cgagaatagc agttcctaag acaattgaag tcccttggaa
    13081 gttataccct tcttgaatta cctgcttgaa ctgttcgttg cggtccacaa aaaatgattt
    13141 aatttgtggc aaagatacac gttatctatc gtggattact gatatggcca acaaagcaaa
    13201 aacagccttt ttttgtcgaa actgcgggtt tgaatcttcc aaatggctgg gaaaatgccc
    13261 ttcctgcaac gaatggaaca cttttaccga ggaggtgata gaacgcccca aggatgcggc
    13321 ttcgtggaag gatagcaagc ctgaaaaacg catttccgga ccggtgcaat tgtcggagat
    13381 caccgcaagc gataccggaa ggctggtaac cggcgatctg gagctcaaca gaacattggg
    13441 aggcggtata gtggcaggaa gcctggtact gattggtggt gaacccggta ttggaaaatc
    13501 aacattactt cttcaattgg cactcgaact gaagcaaatc aaggttttgt atgtttcagg
    13561 agaggaaagc gagcaacaaa taaaaatgcg tgccgacagg ttgaagggca ataatcaggg
    13621 attatatatt tctacggaag ttgccatcag caaattgttt atgcaaataa aacaattgca
    13681 acctgaaatg gtggttatcg attctattca aacattggta agtgattttg ttgatgccac
    13741 tgcggggagt atcgcacaga taaaagaatg tgccggtcaa ttacaacgat tcgccaaaga
    13801 aacaaatgtg ccggtattta ttatcggtca tattaataaa gaaggttata tcgccggtcc
    13861 gaaaattctg gaacatatgg tggatacggt attgcaattt gagggtgaca ggaattacac
    13921 tcatcgcatt ttgcgcacca taaaaaatcg ttttggttct accagtgaga tcggcattta
    13981 taaaatggaa ggtgatggat tacaacaagt aaataatccc agtgaattat taatcactga
    14041 aaaagaagaa cctttgagcg gtgttgccgt tgctgctaca atggagggta tgcgccctat
    14101 gcttattgaa gtacaagcat tagtgagtcc ggcagtatat ggtaatccgc aacgcacagc
    14161 tacaggattt gatttacgca ggttgaatat gttgcttgcg gtgttagaaa aacgcggtgg
    14221 ctttgcctat gggaataaag atgtgttttt gaatattgcc ggtggcttgc gtgttgaaga
    14281 tcccgctatc gatttgagta tcgcatgtgc attgttgagt tcactgactg atatggcatt
    14341 accactgaat atgtgttttg cagcggagat aggcttaagc ggcgaattgc gcggagtagc
    14401 aagaattgag caacgcataa ctgaagcaga aaaattaggt tttgatcgca tttacattgc
    14461 caagcacaac atgaaatcgc tcaagcaaaa taaatacaat attgaattga gggcagcagg
    14521 aaaattggaa gaagtatacg gtcagttatt tgtgtgatga attttttcca cattgctctt
    14581 atttgaaccg gcggatattt catgagcata aattcgcata agcgtttata agcgcgttaa
    14641 tcgacatttt gttgatttta ctcctacact tccatttcga tagtgactaa attatcgaaa
    14701 tgtttccctt attactgtta aatgaattgt cggatatgtt ttatccgcgc ttatgtacgg
    14761 catgcaaaga gccattgctg gcacatgaac atattatatg tttgcgctgt cggattgaat
    14821 taccgcgcac cgatcatggc ttatttcatg acaatccggt tgccgtaaaa ttctggggaa
    14881 aggtgccgat agaggaagca gcggcgatgt tacattttca caaaggatca cgggtgcagt
    14941 cgctcatgca tgcactgaaa tataaaaatc gaacagatgt agggattacg ctgggtaata
    15001 tgttaggata ttattgcaaa caatatcatt tgtttaaaca gattacagcg ataacagcag
    15061 tgcctttgca taaagaaaga ttggcagttc gcggatacaa tcaaagtgct gtaattggcg
    15121 aaggtctggc tgaggtattg caagttccat ttaaaggtga atggctagag cgcatcacac
    15181 ctactgctac gcaaactaaa aaatctcgat tggcgcgttg ggataatgtg aaagatgtat
    15241 ttcgtgtaac tgatcccgat ggaattgcgc atcagcatat tttgctcatt gatgatgtaa
    15301 ttacaaccgg gtcaactttg gaggcatgcg ccttaaaact catacaatct gtaggggtga
    15361 aggtgagtat agccgcgatt gcctttgctg atcgctgagg cctggacttg acatacatgg
    15421 gcaaaaagca gcttgtttgg gaaacacgcc tgattccctg aacaatcgaa aacaggccta
    15481 aaactcaata tttacaggcc ttttgcagga ttttgaccct aaaatgcaaa aaaatatgtt
    15541 aaaatgtttg cgcaactgtt tggttgctta tatatttgca accaattagt tgcgcttaga
    15601 gttttagtat tcgctattac aaattcaaag cccggatact tgacacgcac aataaaattt
    15661 taacaaccca cgcatgagtg aaaaagacaa atggatcgcc atctttataa aaatatggga
    15721 atcgagattc gatcagttag acgtgatatt gcataaactc aaaaataaaa gacaatgaac
    15781 aaaactattc tattcgactt caatgtcgac aaagaggcac gcacgatacg tgtcgaccga
    15841 tcatttgatg cgccgcgaga tcttgtatgg gcggcatgga cagaatcaga tattttagat
    15901 caatggtggg caccgaagcc atatcgaaat gtaacactga gcatgaattt tgttgaaggc
    15961 ggtcgctggc attattatat gatctcgcca aaggatgaga aacattattg cctgtttgat
    16021 taccatcgca ttattccgga aacgatgtac tcaggcatgg atgctttttg cgatattgac
    16081 ggaaatattt cggagagtat gccgcgcatg caatgggaaa acagtttcga aaaacaggga
    16141 gatacaacag tggtgcatgt gcttatcaac tttaataaaa ttgaggacct tgaggcagtg
    16201 atcagcatgg gctttaagga aggatttgct atgggtctgg agaatctgga cgcctatatc
    16261 gcttctcaat tttatcttcg gaaatcaaaa aaaacaagta atgccgcacg tgtatgcact
    16321 tatttaaatt ttcccggcaa tactgaagat gctatgctct tttataaagg gatttttaaa
    16381 acagaatttg tacaaggcat acaacgattt ggcgatgtcc cttccgacgc gggtgctcca
    16441 cctgttccgg atgcattgaa acaaatggta ttgcatgtag aacttccact tataggtggt
    16501 catatcctta tggcaaccga tgcacctcaa gaaatgggat ttgaagtgaa ggtcggaaac
    16561 aacatgcaca tcagccttga acccgattcc aaagatgaaa caacgcgcat tttcaatgca
    16621 cttgcagaag gcggaaatat aacaatggaa ttacaagata tgttctgggg tgcctattat
    16681 ggtagttgca cagataaata tggcattaac tggatgttga attttcagca acattgatat
    16741 agtcaaacca caatctagaa ccggagtgag cctttcgctc cggttttttt attaaaaaaa
    16801 ctcggtctta taaggatagc gtatctgata ttgttcttta accatatcag tcagcataga
    16861 ccgcagcaat tcgatatttg ttctgttgat ggctgaaata aaaacacatt gagcatttaa
    16921 acgctgcatc caggtgtttt ccagttcact taggatatcg cgctttacat caggcgtgag
    16981 atacttatca aaataccgct cttcgtaagc atccatttta ttaaaaatga gaatggtagg
    17041 tttatcaata gcctctaatt ccttcagcgt ttcattcacc acatgaatat gttcttcaaa
    17101 tgccggatgc gacacatcaa ctacatgtag aagaatatca ctttctcgca cttcatccaa
    17161 tgtagattta aagctttcga tcaggtgatg gggcaatttt ctaataaatc caacggtatc
    17221 actcaacaaa aatggtgtac gatccaacac tactttgcgc actgtggtat caagcgttgc
    17281 aaataattta ttttcggcaa atacttcact tttactaagt aaattcatca aagtgctttt
    17341 ccctacgttt gtatatccaa ccaatgcaac acgaattaat tcgccgcggt ttttgcgttg
    17401 tgtttcattt tgcaaaccaa ttttttcgag ctgttgcttc aacaaagcaa tgcgttgctg
    17461 agctaccctg cggtcggttt caatttcttt ttcaccggct ccgcgcatac caataccacc
    17521 tttgatcctg tcgaggtgac cccacaagcc ttttaatcgc ggaagcatat actgggtttg
    17581 tgctaattca acctgcgttt tcgcctgcac ggtttgagct ctggtggcga aaatatcaag
    17641 aatgagcata gacctgtcga gtacttttac ttttaaagtt tcctcaatat tcctcaattg
    17701 cgaaggcgaa atttcatcat caaaaatgac gatatctatc tcatttgcca ctacataatc
    17761 cattatttca aacaacttgc ctttgcccac atacgacttt gtatcaggat gcgggagctt
    17821 ctgataaaat ttcgctttgg tgactgcacc ggcagtttct gctaaaaatt ccaactcatc
    17881 cagaaattca tttaattgct caagcgtctg gtctttggta accagtccga cgaggataca
    17941 tgtttccgtt tcgttaggtt tgatggtagg tttatgcgcc atattgattt attgccgttg
    18001 gcgatggtag taaaaccacc tatatttgcc tgaagacggc gtttctacca caaaggtaat
    18061 tctttgcaac cgaacgccgc taaactacaa atcgctgcat gcagatcaat ttccttcgac
    18121 ataatgagat cgaccgaaaa aattgggatc gctgcatcga caatagcctc aatgctttgc
    18181 cttacgctta tagttggtat ctggacatag tgagcaatgg acaatgggat gcaataataa
    18241 ttgatgatta tgccgctgta tttcctatac cctttaaaaa cagattaata tacaagcagg
    18301 tttaccaacc attttttacg cagcaacttg gtttatttta tacagataaa ttataccata
    18361 caaaattgcc ggatgtattg aaagctctac ctgcatccta tcgaaaaata cacttgcatc
    18421 taaatataga aaatgatttg ggtgaagtat tttctgctgt tgaaaaacgt gttacacatc
    18481 acatagactt aaacaaatcc tatcaatcca tagcttctga ttataacgag caaattcgac
    18541 gcaatctaaa gaaatcattg acacatcatt tacaagttga aacttcttca gatgtcaata
    18601 ttcttatttc tttgcgtgaa atgtttcttc aagaagcgtt aaagggaaaa caaagtgctg
    18661 cagatacaga ccgattaaaa aaacttatcc aagcaatact cgacaataaa aagggtgaaa
    18721 taaaattcgt tcgcggcgct gaaaatgaca tccaggctgc tgtctgttta cttcaaagta
    18781 acggctacat catttatctg tcgagtgttt caagcgatgt cggcaaggcc aatcacgcca
    18841 tgacattttt gattgacgat atgctcaaat ccaatgccaa tacacagcag accttcgatt
    18901 tcgagggctc tatgataccg ggaattgccc gatatttcaa aggattcgga ggcaaagaaa
    18961 aatggtttag cgttattcaa aaataacgtc agacagcatc aatatgcgta caatggaaac
    19021 tgcgaagcga aagcatttac ttcgtttcta acggcagttt tcacagcatc atcatcaggt
    19081 gccatgatta atctgtcgat ccactcaaca atacgcacca tatcattttc tttcatgcca
    19141 cgagtagtaa cagcgggagt tccgatacgt atacctgaag taacaaatgg cgatttatcg
    19201 tcaaacggca ccatgttttt attcacggtg atatgagcat ctaccaataa tccttctgct
    19261 ttttttccgg agatattttt attgcggagg tcaataagca tcagatgatt atctgtacca
    19321 ccgctgataa ttttatatcc tttgctaaca aatgcattcg ccatagcttg tgcattagcg
    19381 atcacttgtt tgcaataggc aacatatcct tcatcaaggt cttcaccaaa tgccactgct
    19441 tttgcagcaa taacatgttc cagcggtccg ccttgtgtgc cggggaaaac agcgctatca
    19501 atcacggcac tcatcatttt cacttctcct ttgggagtag tttgtcccca agggttagga
    19561 aaatcctttc ctatcatgat catacctcca cgtgggccac gcaatgtttt atgcgttgta
    19621 gttgtaacga tatgacaatg aggcataggg tcgttgagca attttgctgc aattaatcct
    19681 gcaggatgag aaatatctgc caataaaatt gcacccactt tatcggcaat agcgcggaag
    19741 cgggcataat cccaatcacg ggcataggca ctagcgccgc agatgatcag ttttggtttt
    19801 tcggcgattg ctgttgcttc cactttatcc atatcgatgc gacccgtttc ttcctctacc
    19861 gaataaaata cagggcgata aagttttcct gaaaagttca ccggactgcc gtgcgtaagg
    19921 tgaccaccat gtgagaggtt aaatcccaat aatgtatctc cgggattaag gcaagcgagc
    19981 ataactgctg catttgcttg cgcaccgcta tggggttgaa cattggcata ttcagctccg
    20041 aataattttt tggcgcgatc aatagcaata ttttcagtta catctacata ttcgcatccg
    20101 ccataataac gttttccggg atatccttct gcatatttat ttgtcataca gctacccatg
    20161 gcttcaagca cctgatcgct cacatagttc tcgctggcaa tcaattcaat gccttcagtc
    20221 tgacggtgga gttctttctt tatgatatca aatacttcag tatcgcgacg cataattatt
    20281 ttctttgaaa caaaattacg tattcagcgg caatttggta tatgctgata cccataggga
    20341 tgtttaatgt atgcatatag atacatttag tcacaaaaag gggtgcaatt aaaataaata
    20401 cttgttactt taagtggttg cagctatgaa acaattgttt tgaaggtggg tatttttgat
    20461 gattaaagat tcggcaacca ccttaaaaac atatctacac aaatattatc ccaaataaat
    20521 caatttcttg attcttttaa ataaaaaagc ccgacttata gagccgggct tcagatgtaa
    20581 tttggaaatt atttttttta cttcgccagc attacgcgtt cgttgatgct ttggttaccg
    20641 gcagtaagac ggatgaaata catgccattt gcgaggtgat atttattcag gtcaactgaa
    20701 acaatatgtt caccttgagc gcctgagatc agttggtcag cacccagatc ggcgatgagc
    20761 tgaccggtaa gtgaatatat agtcaactgc atgttatcct gtgctgcact caatccgtaa
    20821 tgcacttgaa caggaccggc tgtaacagac ggcacagctg tcattgacac agaaacccca
    20881 ttttcttcga cgcttgtttt caacctgaag tgcgccacgg cattttcaat atcagataag
    20941 gcgaagaaaa tatccggagc aaatggatca gggttggcag tatgattttc gagtgaccaa
    21001 taatctaaaa cgtaagaacc tgtaggagta gctgaaaggt ctatgaaggt gccactttca
    21061 tatgcgcctg cataagggta agctgcagga gaaaatcctt caactgttgc aacacctgct
    21121 cccggaggat cgatgtctaa ggtgaaatca tacatcggca attcactacc tgcaaaccat
    21181 gcaattatgg tatcagtagt tgttaaagaa acagttactg aatcatctgt tgtagatgga
    21241 gaaggcacgt ggtttataaa ctcccaatga tcgaaagtaa atgcaggatc tgcgatagcc
    21301 tggaaaccta aagtggtgcc gctgaaataa gtagctgaat aaggataagt atccggaata
    21361 atggtactta cgcgcacatc attcggactg ccggcaggtt caattttcac aacaatacca
    21421 taggccgtta cgtcgaaaca atcttcaaca cctgatggca ggtatgcaca acgttctgca
    21481 ataaaatcgt gcaattcatc gtagttattg ctccatcctg tatatgttcc gccccattta
    21541 tcggtgtgtc gagtcatttc aggatcgagc actgcgcgca tgctatcgat acgtggcaag
    21601 agcacctcac aactaaaggt agtattgatc aaatcagcaa aacgattaat ataatctgaa
    21661 tggaaatccg ggttatccaa taatgagttt aacatatcaa tatgtccatt ggggtcaaca
    21721 ccaccaagtg taagtggatc gcaagggctg gcagttggtg aatcatcagg aacgccggta
    21781 taattgatat agtgtccgaa tgtagcatct tcatcccaca atgcatagcg ccatgtgaga
    21841 ttcgaacctg ttgcatcata gccgcgccac caggccgtat tccagttcaa ccagtcggta
    21901 caaaccgaga atgtatttgt taatacataa tcgatgagac tcatggtgtt aaaattatct
    21961 tttacataat cgtaattagc gggaatggtc atatcgttgg tagtaacgaa tgtaacgaac
    22021 gggctccacg gagccatggt accgtattct gcccatgttc caccccaaca tttgatataa
    22081 tccatttcat attctccctg accatagtag tagtcggtat aatcggcgtc atcagctttc
    22141 tcgcgggtat cgtatacgcc ccaataatcg ccattgagga acatgatatt aaagaaagtt
    22201 gaacgttcat ctaattcgag gccatccaaa cctgaaatat gatgaacaaa agcatcgcgc
    22261 acatgggcgc caccgcttga agatggataa ttgtcatttg cagcggcttt aacaatcaat
    22321 cgctgatacc cttcgcgatc agtaacgtcg ttaaaaaagg tttgatcgat atcattatca
    22381 taaccgaatt gatcgcgaac aataaaatcg aaaccacgtt gatcgtaagc ccatgaatcg
    22441 tttccatgtt cgttaaaatc accaacagct tcatctatga gattaaaatc tttatcgaat
    22501 aattcaaaag atcccacagg ttcaattccc catcctgttg cgttcaataa atcatcaacc
    22561 tggtcaccgg caactgaaat aacgggcata gtagttgtct cgtcaatgaa ataggtattt
    22621 gtagaacaga aacttgccag tgtatcggct gaagccgcga atgccacagt gcgcagaact
    22681 gttgtttctg tgatagtaat cggacctgta taagcagttg atgccgcggt aggaaggttg
    22741 ccattagttg tatagcgaat cgtcattccc ggttcgggag cagtaatagt aacagttaca
    22801 gaacccgcaa aattgccgga attcggttcc atgtcaggct tatgtgcata tgctgttttg
    22861 ggcgttgttg agttagatgc atttggcgtt gggtctgtaa atattttcca atctgctccg
    22921 ccatttggat agcgtcccca actgtgattt gtttgattag gattatttaa ttcgcgtgta
    22981 tccaatactg ttcctgcagc gtcagcaaat accacatcct cagctccggt agtttgtgtg
    23041 agcttaaaat tagtatggta atttgtgcct actgcggtat tcctgttcga acaccaaaag
    23101 cgcaaatagc cacctgcaga aatattcact ccgggcggaa tggtgtattt tgtcggtgaa
    23161 cctattctgt cgctcaggtg atatcctgaa agatccactg ctgttgcacc ggcattgtat
    23221 aattcgatcc agtcttcata ttcaccgtaa ttatcggcga tatcgctgta gttggctgca
    23281 gaatattcgt tgatcaccac ctgacctgaa agaccaagta ttgtcaatcc aaatacacta
    23341 cctgagagga gtaatttttt gagcatacga tgtatgtatg aatgttaaag gcaaactaat
    23401 tcaacctcta aaatacgtaa cattttgata attaagattt atcaatcgag gattttgtaa
    23461 ggcatttgtc acatttggct aaaaaaatgt aagaatatgg ctccagaaag cctggccaag
    23521 ccctttctta gcatggttta aattctacct ttgtgccatg aaagtgatca tcccggtggc
    23581 aggtgtaggc acccgattgc gcccgcatac ctattcgcag cccaaacctc tgattccggt
    23641 ggcaggcaag cccatattgg gtttcatcat cgacaggatg gttaaggcag gattcacaga
    23701 attcattttt gtgataggat atttgggaga aaaaattgag tcttatgtgc tggaacatta
    23761 ccctgaatta aaatgtcgat ttgtcataca aaacaagcgt gaaggtcttg gacatgctat
    23821 tttccttacc aggccattaa tttctgaaaa tgaagagttg tacattgtgc tgggagatac
    23881 catttttgaa gctgatattg aagctgtgat gaccatgccg gagtcggctc tgggactgaa
    23941 acgggttgat gacccgcgcg attttggggt tgccgaagtt gaagacgata tgcgcattac
    24001 aagggtgatt gagaagccct ccataccgaa atcgaatttg gcactggttg gactttacaa
    24061 gatcaaagaa agtgctcagc tgttcaatgc cctggagcag atcatggcac acggttataa
    24121 aacgcaggga gaatatcacc tgactgatgc attgatgatg atgataaagg atggtatcac
    24181 attccgcggt tttaaaatta ccaattggta tgactgcgga aaaaaggaaa tattgcttga
    24241 aacaaatgcc atactgctga aaaaactgca tcctcaaggg cagcatcaac atgcctatcc
    24301 gaataccatt ctcatagagc cggtaagtat tgctccgggt tgtgatatac gtgattctat
    24361 tattggtccg aatgttaccc tgggagaaaa tacatctgta cactacagca tactccgaca
    24421 ttccattatc ggcaacgatg ccaccattga tgatgtggtg ttgctcgata gcgttatcgg
    24481 ctctgatacc gtaatcaaag gcatgagcca gagcctcaat attggcgaca atacggagat
    24541 cgatctttcg aagagtgggt gagtggtgag tggtgagtgg gtgagtcgtg aatggtgagt
    24601 ggtgagtcaa gggtaaatcc atggaatatt acacacgcac ctccctgcat tcaatcggct
    24661 aattggctaa tcggctaatt ggctaattat ttgcacatag agcgcagcga catcccgccc
    24721 tcagcgggac ttccaatatt tcacactctc acttgcacac ttgcacactt gcacactcgc
    24781 acactagtac actcgcacac tctatacttc tatctttgca aaaatctttt ctcatgcaca
    24841 atcgcattac agcattattc ggaatagagt atcctatcat tcaggcggga atgatttggt
    24901 gttcggggtg gaaattagcg agtgctgttt ctaatgccgg tgggttagga ttgataggta
    24961 gcgggagcat gtaccctgat gtattgcgtg aacatattca aaaatgtaag gccgctacca
    25021 acaagccttt tggagtaaat gtaccgctct tatatcctga tattgacaag cacattcaaa
    25081 taattattga ggagggtgtg aaaatcgttt ttaccagcgc cgggaatcca aagacatgga
    25141 cttccatgct caagcaacat ggcattaaag tagtgcatgt agtgagcagt cgaaagtttg
    25201 ccctaaaatg tcaggaggca ggatgcgatg cagttgttgc agaggggttt gaggcgggtg
    25261 gtcataacgg tcgcgaagaa accactactt tatgcctgat tcctagtgtt gccgacgctg
    25321 tcacaattcc ggtaattgct gcaggtggaa ttggcgatgg cagggctatg ctggcgtgca
    25381 tggttttagg agcagaaggg gtgcaaattg gcacgcgatt tatcgccagt atcgagagca
    25441 gtgcgcatga cgcattcaag caaaccgtta ttcatgccga tgaaggcagc accatactca
    25501 cactgaagga aataaccccg gttagaatgg ttcga
//
LOCUS       DAOQHY010000025        25028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_869_length_25028_cov_5.307212, whole genome shotgun sequence.
ACCESSION   DAOQHY010000025 DAOQHY010000000
VERSION     DAOQHY010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 25028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25028
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_869_length_25028_cov_5.307212"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PLL28_03580"
     CDS             <1..321
                     /locus_tag="PLL28_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68428.1"
                     /translation="DSLYIFSRNGDPEYHMTWEFKLCNDTSGLLSTCEPQIIDLMTKL
                     KIKEVQYVGVSGRKSMEIPIVIPDTIGSDDYRFSILNLFRPPKNTDDSLGFWFLRMYD
                     FSMF"
     gene            529..1290
                     /locus_tag="PLL28_03585"
     CDS             529..1290
                     /locus_tag="PLL28_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68429.1"
                     /translation="MKLILSILLLSLAANSFGQNLEPYNAEFKFQVVTGNNALRADST
                     VEAIIIVCDSVSIKNAYHSVEENLQIKELKLINPDQMLINEFASLELKNLHYLFIDAY
                     RHESLQISKFPNVEVFSVTSERLEKLDMVGAQFQKLAVLGVHAVELREWTSDSALPSL
                     ELLDIMAPHLDSLPILLMPQLYEFVFFCSFTSMPKDLCKYEELSLMSFNNFTAIPVDD
                     CIIEKIKSGVYSNLTVFESISGKELLKVVSNDRKE"
     gene            complement(1342..2817)
                     /locus_tag="PLL28_03590"
     CDS             complement(1342..2817)
                     /locus_tag="PLL28_03590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68430.1"
                     /translation="MKNRLLLIVLTGLLSVNAQAQWMLDSANTAGTYPYAAVNGNKAV
                     FSNGGEWNVFDAVTGVHTYGNFAISRAMIEVVSAGDKVMFAGGKYGYFADPQYTKSVN
                     VYNATTNTWSTWNMGTAREVGGAAAIGGKAVFAGGTGRSDIAGPVYMYNKVDIFDVAT
                     GARTSGKLSKGRSNIAAGAAGNKIVFAGGWYWDMMYSVLPANTVDIYDVVTGVWTKTT
                     LSKKRENISVAVIGDKILFAGGTGSMGAVTNVDIYNSTTNTWSVSYMPAAGDGMKSAV
                     IGSDAYFGGGITGPGNMLYKYNSVAGTWSSITIPTSLTGFSMSVINGRLIIAGGTIPG
                     TSTYSDLVQIYDPVANSWYNEHLSIARTNVSALTIGSKGYFAGGISVYGYPTPTYTKR
                     VDIFNAPFRMEQSIASEISNWDVQVYPNPAVNTVHIAIQDAFDQALIATITDINGNLI
                     QQFQLDATVQNIDVSELPSGIYFIIVQDNSARSIVKKLVKQ"
     gene            complement(2978..4267)
                     /locus_tag="PLL28_03595"
     CDS             complement(2978..4267)
                     /locus_tag="PLL28_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68431.1"
                     /translation="MKSRLITCMLFFCSMTLQAQNNSTCSFHGVVVIGSEEALLQGAY
                     LKCLGTGDDVITDDEGNFSLRVSCSGPDTLILQSISGSEKFLLQDGKYTDDTLRIVFK
                     LYNLNDIFVTPKEPVDIVRKAVARIPINYPETSHVAFGFYRNYKLINNRFRELTELQM
                     AVAMRFEEKGQHNELREAYAVDKIRRTLYTISIDEFYKGQLTDLFTQNLVYHTDFAFF
                     NNIFIKQCDFSFDSTSTDSTWVINYKLTNITGENHGVSNYSPVDFYGEATETGYLVIN
                     KSDYAIIKLVRESLRNKKYEYPKRNNFLLPDLQYSEQFHEGFLECNYAMFDGKYYPVS
                     IFHAYKNTFTHNPTNSIAYIITDYSEWHCDSISEMIDPVWMQRFDKFTALERMYYTYD
                     PNDWTQTPSWYFIRNEQVYDDLQLIDDPEILFKKSGE"
     gene            complement(4449..6707)
                     /locus_tag="PLL28_03600"
     CDS             complement(4449..6707)
                     /locus_tag="PLL28_03600"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HNF68432.1"
                     /translation="MAFDLDMIRQVYAAYPARIEAARKLLNRPLTLTEKVLYAHLSEG
                     MASKAYERGKDYVDFGPDRVAMQDATAQMALLQFMSAGKKKVAVPSTVHCDHLIQAKT
                     GADIDLATALDSNKEVYDFLASVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTD
                     SHTVNAGGLGMVAIGVGGADAVDVMAGFPWELKMPKLIGVKLTGKMNGWTSAKDVILK
                     VAGILTVKGGTGAIVEYFGDGAAAISCTGKGTICNMGAEIGATTSIFGYDDSMRRYLQ
                     STDRQDVVDLADAVAEHLNGDAEVYANPAAYFDQLIEINLDELEPHVNGPFTPDLAWP
                     LSKLKDAVITNGWPATLEVGLIGSCTNSSYEDLTRAASLARQAAEKKLKAKAEFTITP
                     GSELVRYTVERDGLLEDFEKIGGVVLANACGPCIGQWARHSVDPNRKNTIITSFNRNF
                     AKRNDGNPNTHAFVASPEIVTALAIAGDITFNPITDTLVNEEGVAVKLDEPTGLELPA
                     KGFAVEDAGYIEPAADGSKVEVLVDPASKRLQLLAPFTAWEGTDLKGLKLLIKVKGKC
                     TTDHISMAGPWLKFRGHLDNISNNMLIGAVNFFNDKTDSVKNQLNGQYDAVPAVQRNY
                     KANGVGSIVVGDENYGEGSSREHAAMEPRFLGVRAILVKSFARIHETNLKKQGMLALT
                     FADKNDYDKIQEDDNIDIIGLTTFSPGQPLTLRLHHSNGSSDDIIVNHTYNAQQIEWF
                     KAGSALNLIGDK"
     gene            6917..8851
                     /locus_tag="PLL28_03605"
     CDS             6917..8851
                     /locus_tag="PLL28_03605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68433.1"
                     /translation="MNRYTITAYSATAGIFLLHSNVTDAQMVHVNVDPDAHVGPYFDY
                     NLDLNADGINEVFFNCGEFWTGSSFGGEFHGYSSQWIYLYPTGTFATSIGQIKHLVVG
                     DTVDADMLFNDVDPLFFYGQRDIIAGGGWVDIVEGSEWVNNEGFFGLKFPIDGETHYG
                     WIRVELIQLDPDYLPQMYVKEFAYNDTPDAPAPITIYNADIAKSLVLSDVGETNTPED
                     LQLTFNKADNEITVSEYRVALFKGAIPPTVAQIDSLTPDRYISVFPTGDDLSVSFSAD
                     ILDINGDTIIPSHLYRAIVLSVADGTYAIESDLSVPSNYTYFSLDVAPATYEVELLTD
                     SIATDISGFIGSFGMMDTSVSAVRLYITANEMNVEELLALDEPYYMETIPVVGINTVH
                     FTSDKLIYEAGDPALFNLYYLHVVSIPDSITNSIPALSVQSGRFLYNQYPVAPEITII
                     GYAGNSSDILLEFPQLPDEASLEVYRVFVCKAGTVVNSELVAGMLPIHYESVYPSGVD
                     LHMDLTACNLDSDGDEIIEGQPYVAYVAMRCTCDDYFYSITIPSSEFILGEPEVGIDN
                     MATDELIITDNVILIPNGLESSTLQISNASGQMMFEYMIQSGDRDIKLPPFPAGIYIA
                     YIKNEQHIYAKKFVITSSGG"
     gene            8926..10836
                     /locus_tag="PLL28_03610"
     CDS             8926..10836
                     /locus_tag="PLL28_03610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68434.1"
                     /translation="MKTLTLSAYSAASALLLGLSKDTSAQMIYQDLDPDFGIGPDFPV
                     DLNDDGISEMRIHGHGWWTFTSDMVYSSRQIEFFNYAEIATSAGVLQMFEGDTVDADL
                     DFGTADQFFFYGYNDDGWIDIDEGASWVNNNSYIGMRFDVSGEIHYGWLRVELHQDDI
                     HDMPTLYVKELAWNATADAPAPINAHVGVALFPELSDVGETNTATDLQLHFQKADDES
                     HISSYRVILYPGFPAPTLAEANALSADRYTELIPVGADITMNFTETTRDIDGNLLLPG
                     TFYSAIILSVADGIAVTENDLSSHSNNMQFIYLEAPVPLDVYLQSTGYDDDITGLYGR
                     FYLTDTAVASVRAYLSDSYFEADELLLLDPEYYIETIPDAVGTHYMWFTADKHIYTSD
                     APILFEDYVLSIVTVPNGITNSIPELGTDVAHFTYPNYDVMPTVTLVDSTGTGADIQL
                     HYPMFINEDDLELYRIFIAKADVGIEPAYAYTVPPSKRLDISPAGIDLDVNLSSITLD
                     TDGNSIKYNQAYRVYVALKGDFYPDYYSLATPSAEFILEEPVIESIFSQNNKAAPNIW
                     VNDHLIHIAGATNEMHTLTLLNASGQEVYRTTFNGTEVAVEAPELPAGVYVVGVESEH
                     GVHHQTIFLQGK"
     gene            10923..12149
                     /locus_tag="PLL28_03615"
     CDS             10923..12149
                     /locus_tag="PLL28_03615"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF68435.1"
                     /translation="MTLRENISLAFRAIKGNRLRSVITITIIALGLTALIGILTAIDA
                     LESSINSNFAMLGANSFSIRNFNELSNGNEKPNPPISYKEATSFKDRYTYSGADVSIS
                     AIFIQGSAEVKQAAKKTNPNVDVYGVDENFIPVVGYTIKEGRGFSNIELNSATRVVVL
                     GSDVAEKLFGERDNPIDKGVTINDQYFRVIGILEPKGSSFMQSDNRVLIPLTAARSIF
                     PQVGRDSYILSVGVKTAVDMEPAIGEATGLMRQVRRLRLGEDDDFSIAKSDSVVSIML
                     DSLSYVALGALFIGFITLVGAAIGLMNIMLVQVNERTREIGVSMAIGANRKVIRRQFL
                     IESIVICILGGIFGIILGIAIGNLLAVALHANFILPWLWIILGLTVCVITGLAAGYIP
                     ARRASRLDPIEALRYE"
     gene            12582..13499
                     /locus_tag="PLL28_03620"
     CDS             12582..13499
                     /locus_tag="PLL28_03620"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68436.1"
                     /translation="MMLHTNSKLLQYTTLASTVLIGEKITAQSVYVDIDPDVPLFEDV
                     WSDEGWKYFDLNEDGILDVGLSYHVGFICGYCPYVIGFDVALLNGAEIATMIAPSLSL
                     WSVYQSSYSSSVGACQIPSHAIADGLAEGEVINMDNQFGVIEELFQTFRCGSYGDFGV
                     QNDLDFQDHNWSTYSKPYIGFQLTVPLGYQYAWLRLYEGDDYNIYVTDFGYQSHVNAG
                     LMIEQPVTDIDESKPQSPYLFSSNGVVHVQDGTGYLMTVTDMQGKEIVEENITSSNFE
                     KRYDLQMGIYLITIVKGDSRFTEMLMLNN"
     gene            complement(13555..14001)
                     /gene="rplI"
                     /locus_tag="PLL28_03625"
     CDS             complement(13555..14001)
                     /gene="rplI"
                     /locus_tag="PLL28_03625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNF68437.1"
                     /translation="MEVILLKDVDNLGDANTLVKVRDGYGRNFLIPRGLAVIANEGNR
                     KMMTERQKAEEARERKLVARIQEVIATLQNGNIRVGAKVGQTDKIFGSITNVQLAEAI
                     KKQTGLVIDRKKITIADEVKTLGTFSANVMLHKDHIVPVSFEVVEE"
     gene            complement(14009..14224)
                     /gene="rpsR"
                     /locus_tag="PLL28_03630"
     CDS             complement(14009..14224)
                     /gene="rpsR"
                     /locus_tag="PLL28_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNF68438.1"
                     /translation="MRKKYCRFKKSGIKYIDFKNPEFLLKFVNEQGKILPRRLTGNSL
                     KYQRKVAQAIKRSRQLALMPYVADLLK"
     gene            complement(14278..14661)
                     /gene="rpsF"
                     /locus_tag="PLL28_03635"
     CDS             complement(14278..14661)
                     /gene="rpsF"
                     /locus_tag="PLL28_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNF68439.1"
                     /translation="MNQYETVIIFTPVLSDDDVKRTEESYKDFLRSNGAEIVAEERWG
                     LRQLAYKIKKKSTGIYFVLEWKAPTDVTGKLDIQFNRDENVLRYLTVRLDKYSMDYND
                     RKRRGEIGRKANAKKESGKLQEEGA"
     gene            14789..15643
                     /locus_tag="PLL28_03640"
     CDS             14789..15643
                     /locus_tag="PLL28_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68440.1"
                     /translation="MHQIRASITLIPVLAVAVLAGCKKESLDTDTYIALDNAIADGSF
                     QDMMNVVTQQAVANGIDGFSSGTANGKLLEDCPTTTFSEPLGTFPNTMTVDFGVSCTA
                     GYLGVERSGKVIATFTGPYSAEGTTITITTDNYYVNGNKVEGTKTITNEGLNDAGNLE
                     FVVVMVDGVVTLATGETISWNSSRTREWIAGDETLEIEDDVYSVTDGPGVDNAVEGIN
                     RSGTPFTAHIAEPLVRALDCRWFRSGVLEVTPEEMLTRSIDFGSGDCDNVATLSIGEF
                     ETEITLPY"
     gene            complement(15721..17340)
                     /locus_tag="PLL28_03645"
     CDS             complement(15721..17340)
                     /locus_tag="PLL28_03645"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF68441.1"
                     /translation="MKIIKLLLVCLLIQGVAFGQTISDAKHAIESEFYFKAKRMLLNM
                     NKTAPSVESNFYLAEVYLTTGNTDSAKYYYKAASEVTDSKNPLIYVSRGKMLLLNNNA
                     TEAQAQFESAIKASKGKNAEIIHLIGDAYMVAGNVTEAISYYDQAYSTDPNLVINLLA
                     YGDAYLSQDKPGEAMTKYEQAKAVNPNIAVTFLRIARINSKTGKHDAAIAAYKEAIRL
                     DPNLAIAYKELGEESYLAGYYTDVKKYFDEYVKLNAEDKEARLVPAVTCYQLKDYACA
                     IESATQIIAIDSTNFVAWRILYFSHYELGDSIRKTEPDASAKHFADGYQASQKFWSIG
                     EKKLVTLDYQYSAQLAVEAKDTAKAIFYYTLATGSDSTSTYEIAAEYAKYLYTIKKYA
                     ESIVAYNNVIAKYQAGALDYYFLGRAYYQTNDYVNADTIYAQFIALQPNSPDGFLQRA
                     KTKVRLDGIDERGTALPLYLRYIELAEKDIEKNKKNLIDAYLYCFSYYDLMKQTADAC
                     TNFNKAKTLDPNNEYVKQFDAQVNCPTGSSD"
     gene            complement(17363..18268)
                     /locus_tag="PLL28_03650"
     CDS             complement(17363..18268)
                     /locus_tag="PLL28_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNF68442.1"
                     /translation="MNKLNLLLIITFSLFLGACKDKAWDERTENLNKGTITLYAESSY
                     QTLVEELVKSYENVYPESKIEVRFAPDNAVLDAMFTDKTRMIITGTALSKEQLDRIKS
                     VNEVAAEQYVIGREAIAVIASLSNPDTVFDFDAFLQSRSAGYSGVYEKTGFVFNEENS
                     AMVSQLLGNMTGYNNMFSLGNLDTVVGYVSKNTAAIGFISFAALSDPDDSLTQAILRQ
                     VKVLPVAKADSTGKKIVIPMSQSSIASNDYPFQRYVTVVKGNTPELLGTGFVNFLYRS
                     KASRIMLKAGLVPEKMPERRINIVE"
     gene            complement(18255..18500)
                     /locus_tag="PLL28_03655"
     CDS             complement(18255..18500)
                     /locus_tag="PLL28_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68443.1"
                     /translation="MRNSIFRHPVLLLKLVASVMYVTMGILVLLVPDMLSNMLEGITP
                     LLVNLFAALLIVYGFYRLYRVVTELSEINKPDNDEQA"
     gene            complement(18567..19376)
                     /locus_tag="PLL28_03660"
     CDS             complement(18567..19376)
                     /locus_tag="PLL28_03660"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNF68444.1"
                     /translation="MMEDNKKYLNQSMDEIVFDNRNKSYGAYFLRQLTKRNTVKATIV
                     MFVVSGMLTGFTFVDFGFLTKKNDDEAINRVVTLTEPPPLDEAAPPPPPPPPPPPPVR
                     PTVRFVEMVAQRDEDVAEDETITQEEITAEISTQTQEGDEDADIVIETAPVVVDKPVV
                     QEKKVYEFIEQMPEYPGGTEALYNYINSHIQYPRMARDNGVQGKVFLKFIVDENGVVS
                     DVQLLRGIGAGCDEEAMRVVRSLPKWTPGKQNGKPAAVWYKLPIQFTLEDN"
     gene            complement(19440..20003)
                     /locus_tag="PLL28_03665"
     CDS             complement(19440..20003)
                     /locus_tag="PLL28_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNF68445.1"
                     /translation="MADIQTPDKGSHSKHGKGKVRSKKMSTRVDFTPMVDLAFLLISF
                     FMLTTTLNKPVAMELNMPKKDETVKTDVKESQVLNVILDKEDKIWYYDGRVVYDMQKT
                     DFSADGIRDVIYKKQKEVGQKFGDPKKTIVIIKMTEDANYKNMVDILDEMDITDTKIY
                     AIQDVADLELEAIANGGKLDVIPEGLQ"
     gene            complement(20013..20702)
                     /locus_tag="PLL28_03670"
     CDS             complement(20013..20702)
                     /locus_tag="PLL28_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNF68446.1"
                     /translation="MPKIKVKRQSTIVDMTAMTDVSFLLLTFFILTAKFRPAQPVIID
                     MPSSESETVVPDNKMVISVDKEGKVYYSIDNPKVRVATLQQLIERYEKTYPSLSKLTD
                     AQIANFANVEMLGSDINQLDQLLNMPPQEINAMHDFSGVPIDSTNNQLRDWIQAGRWA
                     DQIDKTESGTKRDDIRVAIKGDKTSSIKTIQEVIKILTEEPVNIHTFNLITTLEGPVA
                     GGVEASPTPAK"
     gene            complement(20744..21550)
                     /locus_tag="PLL28_03675"
     CDS             complement(20744..21550)
                     /locus_tag="PLL28_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNF68447.1"
                     /translation="MAQSAAKPAKKTSMDGLGIIVIAVNLVIGILFYKFVCGNPDNFK
                     ANGDPKEGNFLGIVFKGGFIVPVLFALFLTVLVFAFERLLTISKAKGNGSIQSFLQKV
                     KYHLANKDIDAALKECDRQKGSVANVVQAGLHKYAEMKSDTDATTEQKVINISKEIEE
                     ATALEMPALEKHLPILATIASIGTLIALLGTVLGMIRAFAALASEGTPDPSALSAGIS
                     EALVNTAIGIGTSAFAIIFYNIFTGMIDKLTYGIDEMGFSITQTFSAQEK"
     gene            21868..22944
                     /locus_tag="PLL28_03680"
     CDS             21868..22944
                     /locus_tag="PLL28_03680"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNF68448.1"
                     /translation="MSRLKLSPGAFFASGTGRVILHVVFWILYMNSPIFMMEVNTFSS
                     NGILLFEVMNLLFIPYYYLLANVLIPKLFTLKRSPLFVVSWVGSYVLMAYCVQQVSIH
                     FLYTLTTEAEIATFKKVAETSPTYFPEFFRMIFVTMVPLSIMLVRRYARISAKQTELK
                     RMNADLELNFLKSQMNPHFLFNSLNNIYALALQQSEKTPGLILQLSDLMRYMLYECNA
                     AQVALDKELNFLRDYIGLEKVRHGEKVKIEFNIKGETSQKQIAPLLLLPFVENAFKHG
                     VNAQLGPAWVSVDFNAEDPEGGCTFVVRNNKPVKTEKTSSQAGGIGLENARKRLDLLY
                     PNKYLLHVDNHNEVYQVELKIMDL"
     gene            22941..23639
                     /locus_tag="PLL28_03685"
     CDS             22941..23639
                     /locus_tag="PLL28_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF68449.1"
                     /translation="MNHKLRCIVVDDEPLAQQVIVTYIGRLSHMELVATCDNAMEAMQ
                     ALQKDKIDVMFLDIQMPVITGVEFLRSLQHPPRVIFTTAYPDFALDGYDLNITDYLLK
                     PISFERFMKAVNKLSEAVNNEEHNTPIPDHFFVKEDGKLVRVNFADIDHIECMKDYAK
                     IFTKQRMIVTHHSMKKFEEVLPDQQFMRIHRSYIVSLSSINAIFGNVIETAKGRLPVG
                     ANYREMLMEKIKVD"
     gene            23684..24154
                     /locus_tag="PLL28_03690"
     CDS             23684..24154
                     /locus_tag="PLL28_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68450.1"
                     /translation="MKKILTLVLAFTFFSQAHAQINDHAIGIRFGFGGGISYQQKMSK
                     TNRLELNGSANFYNGYSAFRLTGVYQWVWDMNKGFSFYAGPALSTGTMSLGDNYNGKG
                     KNGFFVSGGGQVGLDYNFDGLPLQFSVDVMPLFPIINNVDDFYLDPALGVRFVF"
     gene            complement(24212..24754)
                     /locus_tag="PLL28_03695"
     CDS             complement(24212..24754)
                     /locus_tag="PLL28_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNF68451.1"
                     /translation="MQQYYPYFLAAHIIFVITWFAALFYIVRLYIYHTEALEKQEPER
                     SILAAQFTIMEQRLMAIIGTPSMILVVITGVSLLSIQSQFLKEPWLHTKLLFVIGVIV
                     YHLACLRIQKQLREGIAKNSSTKLRMFNEVATVLLVAIVFLVVLKDLVDMLYGLLGLI
                     ALSFSMLVAIRIYKRRREKR"
     gene            complement(24757..>25028)
                     /locus_tag="PLL28_03700"
     CDS             complement(24757..>25028)
                     /locus_tag="PLL28_03700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNF68452.1"
                     /translation="HVVMPTVFSPNNDGINDWIGPNYSGDIKDYHLEIYNRYGQLIFE
                     TDEIAQGWNGKFENELQPIGTYVYVMQVKLNNKKREERGNITLVR"
BASE COUNT         6908 a         5062 c         5304 g         7754 t
ORIGIN      
        1 gattcactct acattttttc tagaaatggc gatcctgagt atcatatgac gtgggaattt
       61 aagctttgta atgatacttc aggtctactt tcaacatgcg agcctcaaat tattgattta
      121 atgactaaat taaaaataaa agaggtgcaa tatgtaggtg tctctggcag aaaatcaatg
      181 gaaattccta tagtaatccc tgatactata gggtcagacg attatcgttt ctcgatcctg
      241 aatcttttta ggcccccaaa aaataccgat gacagtttgg ggttttggtt ccttcgaatg
      301 tatgattttt cgatgtttta atcttgaatt tgttattgtg acgtggtaaa tattgaaaaa
      361 ttcaacatta aaggcgttga aaagcatatt tgaacccatg tattttgttt taatattgaa
      421 gtttttgtga caatttgtat attgaattta ttttctatca gattttgaac cgatggcagg
      481 ggttttaaaa tcatattagg ttttatttgc tataaattaa aaactgacat gaaattaatt
      541 ttatcgattt tattgttatc gctcgcagcg aattcatttg gacaaaattt agagccatat
      601 aatgcagaat tcaaatttca agtagttaca ggcaataatg cattgagagc agattccacg
      661 gtggaggcaa taatcattgt gtgcgattct gtaagcatta aaaatgcata ccattctgtt
      721 gaagaaaatt tgcaaataaa ggaattgaaa ctcattaacc ctgatcagat gttgattaat
      781 gagtttgcgt cattggaatt aaaaaattta cattatttgt ttattgatgc ttaccggcac
      841 gaatcattgc agatttctaa attccctaat gttgaggtgt tttctgttac ttcagagaga
      901 ttggagaaat tggatatggt aggcgctcaa tttcagaaat tagccgttct tggtgtgcat
      961 gcagtagaat tgcgtgaatg gacttcagat agtgctttgc cttcgcttga actgttagat
     1021 atcatggctc ctcatcttga ttcattacca atccttttaa tgcctcagtt gtatgaattt
     1081 gtcttttttt gctccttcac ttcaatgcca aaagatctgt gtaagtatga agaattaagt
     1141 ctgatgtcgt ttaataattt tacagctatt ccggttgacg actgtatcat tgagaaaatt
     1201 aaatcaggtg tgtattctaa tctaactgtt ttcgaaagca tctccgggaa agagttgtta
     1261 aaagtggttt ccaatgatag aaaggaataa aaaagccacc ccgaaggatg gctctttgta
     1321 tggactacac ttagttcccg gtcattgttt aaccaatttt tttacaatgg aacgggcgct
     1381 gttatcttga acaattataa aataaatgcc ggaaggtaat tcgctgacat ctatattttg
     1441 aactgtggca tctagttgga attgttgtat cagattgcca ttgatatcgg taatagttgc
     1501 aatcaatgct tggtcaaatg catcttgtat ggcaatgtga acggtgttta ctgcaggatt
     1561 tggatatact tgcacgtccc aattactgat ttcggatgca attgattgtt ccattctaaa
     1621 cggtgcatta aatatatcaa cgcgtttggt ataggttggg gttggataac catatactga
     1681 aatacctccc gcaaaatatc ctttactgcc aatggtcaat gcagatacgt ttgtgcgtgc
     1741 aatgctcaga tgttcattat accagctgtt tgcaacagga tcgtaaattt gaaccagatc
     1801 actgtatgtg cttgttccgg gaatggttcc gccggcaatg atcaagcgac cattaataac
     1861 ggacatgctg aatccggtaa gtgatgtcgg aatagtaata gaactccatg ttcctgcaac
     1921 actattgtat ttgtatagca tattgcccgg acctgtaatg ccacctccga aataagcatc
     1981 acttcctatg actgcggatt tcataccgtc gcccgcagca ggcatatagc ttaccgacca
     2041 tgtattggtt gttgaattat aaatatcgac attagtaact gctcccattg aacctgttcc
     2101 accggcaaac aagattttat cgccaataac ggcaacggaa atattttctc gttttttcga
     2161 cagcgtggtt tttgtccaaa cacctgttac aacatcgtaa atatctactg tatttgcagg
     2221 taaaacacta tacatcatat cccagtacca tccgccggca aacacaattt tatttcctgc
     2281 agcacctgca gcgatgtttg atcttccttt tgataatttt cctgaagttc gggcacctgt
     2341 ggcaacatcg aaaatatcta ctttgttata catgtaaaca ggtcctgcaa tgtctgatcg
     2401 acctgttcca cccgcaaata ctgctttgcc tccaatggct gcagcacctc ctacttctcg
     2461 tgcagtaccc atattccatg tcgaccaggt attggtagtc gcattgtaaa cgtttaccga
     2521 ttttgtgtat tgaggatcgg caaaatatcc gtatttacca cctgcaaaca tcactttatc
     2581 gccggcactt accacttcga tcatagcgcg ggaaatagcg aaattgccat aagtatgcac
     2641 accggttaca gcgtcgaata cattccattc gccaccatta gagaatactg ctttgttgcc
     2701 attaacggca gcatacggat aagtgcccgc cgtattagcg gaatccagca tccattgtgc
     2761 ctgtgcattg accgataata agcctgtcaa tacaattaag agaagtcggt ttttcatatc
     2821 ttgagtttta aatgattcaa taaaatttcc ctaagcaaag atgcaagcaa aacaaggaga
     2881 ggcgctatga cttatgtcac acaaaatgct gattcagggc acttttcaga caaatagaag
     2941 ggtagggcgc aggtgaatta atgtccttaa tggaaggtca ttctccactt tttttgaaga
     3001 gtatttccgg atcgtctatc agttgcaaat cgtcgtaaac ttgttcattg cggataaaat
     3061 accaggatgg ggtttgtgtc caatcatttg ggtcataggt gtaatacatg cgctcaagtg
     3121 ccgtgaattt atcgaatcgc tgcatccaaa ccggatcaat catttcgctt attgaatcgc
     3181 aatgccattc ggaataatcg gtgataatat aggctatgct attggtaggg ttatgtgtga
     3241 aagtgttttt atacgcatga aaaatactca ccggataata tttgccatca aacattgcgt
     3301 aattacactc caaaaagcct tcatgaaatt gttctgaata ttggagatca ggtaacagaa
     3361 aattatttcg ttttggatat tcgtattttt tatttcttag actttctcgt acaagtttta
     3421 tgatagcgta atccgattta ttaataacaa gatatcctgt ttcggtagct tcgccgtaaa
     3481 aatctaccgg tgaataattg cttacaccat gattctcacc agtgatgtta gtaagcttat
     3541 aattaattac ccatgtgctg tcagtagaag tgctgtcgaa actgaaatca cattgcttaa
     3601 tgaagatatt attgaaaaat gcaaaatcag tatgatatac gaggttttgg gtaaacagat
     3661 cggtaagctg ccctttgtaa aattcatcta tgcttatcgt atataatgtt ctgcggattt
     3721 tgtctactgc atatgcttcg cgcaattcat tatgctggcc tttctcttca aatcgcatgg
     3781 caacagccat ttgtaactcc gttaattctc tgaatctgtt atttatgagt ttgtagtttc
     3841 tgtaaaaacc aaatgccaca tgcgaagttt ccgggtaatt tatcggaatg cgcgcaacgg
     3901 cttttcgcac aatgtctacc ggctcctttg gcgtgacaaa aatgtcattc aggttgtata
     3961 atttaaacac aatacgcaat gtgtcatcag tgtattttcc atcttgaagt aagaacttct
     4021 cgcttccgct gatactttgt aaaatgagcg tgtccggtcc ggaacagcta acgcgcaaac
     4081 taaaatttcc ttcatcatct gtaattacat catcgcccgt gcccaggcat ttcaagtatg
     4141 caccttgcaa caaagcttcc tcactgccaa tcacaacaac cccatgaaaa ctgcatgtgc
     4201 tattgttttg cgcctgcaat gtcatagagc aaaaaaacag catgcatgtg ataaggcgtg
     4261 atttcattaa acgaatttac gagtagttct ttaatttgtt tcgcttgcca ccaattctga
     4321 cattgcgctg acgtatgtga atttatattc tgaaacaaat tgtatgcctg cttcaagttt
     4381 tcttcatcac catccaaaaa aaaagagtgt ccgtatgaac actcttttta gtatagttgg
     4441 atattatttt atttatcgcc aatcaagttc agtgcacttc ctgccttgaa ccattcgatt
     4501 tgttgcgcat tataggtatg atttacaatg atatcatcac tgctgccatt gctgtgatgc
     4561 agacgcaatg tcaatggttg acccggtgaa aatgtagtca gaccaatgat atcgatattg
     4621 tcatcctcct gaattttatc gtaatcattt ttatcggcaa atgtcaatgc aagcatacct
     4681 tgttttttaa ggtttgtttc gtggatacgt gcaaatgatt tcaccagtat agcgcgcacg
     4741 ccaaggaaac gcggttccat tgctgcatgc tcacgtgatg atccttcacc gtaattctca
     4801 tcaccaacca caatactgcc aacaccattc gctttgtaat tgcgttgaac ggcaggaacg
     4861 gcgtcatatt gaccgtttaa ttgattcttt accgaatcag ttttatcgtt gaagaaattc
     4921 acggccccaa tgagcatatt atttgaaata ttatcgagat gtccgcggaa tttcagccat
     4981 ggacctgcca ttgagatatg gtcggtggtg cattttcctt ttactttaat caacaatttt
     5041 aatcctttca gatctgtgcc ttcccacgcc gtgaaagggg ccaataattg caaacgtttg
     5101 ctggcaggat caacaagtac ttctactttg ctgccatcag ctgccggttc aatatatccc
     5161 gcatcttcta ccgcaaatcc ttttgcaggt aattccagac ctgttggctc atcgagtttt
     5221 acggcaacac cttcttcgtt aaccagtgta tcggtaattg ggttgaaggt aatatcgcct
     5281 gcaatagcaa gagctgtaac tatttccggt gaggctacaa atgcatgtgt attcggatta
     5341 ccatcattgc gttttgcaaa gttgcggttg aaagaagtga tgatggtatt ttttctgttt
     5401 ggatcaacac tgtgacgcgc ccactgacca atgcatggac cgcaggcgtt tgccagaaca
     5461 acgccgccaa ttttttcaaa gtcttcgagc agaccatcgc gttcaactgt ataacgcact
     5521 aattcactgc cgggggtgat ggtgaattcc gctttagctt ttaatttttt ctctgctgcc
     5581 tgacgtgcca atgatgcagc acgtgtgaga tcttcatagc ttgagtttgt gcagctgccg
     5641 atgagcccta cttcaagagt tgcaggccat ccgtttgtga tcacggcatc tttaagttta
     5701 gataaaggcc atgccagatc gggagtaaaa ggaccgttta catgcggctc caattcatcg
     5761 aggttgattt caatcaattg atcgaaataa gctgcaggat ttgcatacac ttctgcatct
     5821 ccattgaggt gttcggcaac agcatcagca agatcaacaa catcctggcg atcggtagat
     5881 tgcaaatagc gacgcatgct gtcgtcataa ccaaaaatgg aagtagtagc gccgatttca
     5941 gcacccatat tacaaatggt acctttacct gtacaagaaa tggcagcggc accatcgcca
     6001 aaatattcca caattgctcc ggttccgcct ttaacggtca ggattcctgc cactttaaga
     6061 attacatctt ttgcagaagt ccatccgttc attttacctg tcagtttcac accgatcagt
     6121 ttaggcattt tgagctccca agggaatcct gccatcacgt caacagcatc agcacctcca
     6181 acaccaatgg ccaccattcc gagaccgccg gcgttaacgg tgtgactgtc ggttccaatc
     6241 atcataccac ccggaaatgc atagttctca agcaccacct gatgaatgat accagcgccc
     6301 ggtttccaga atccaatacc atatttatta gatacggaag ctaaaaaatc atagacttct
     6361 ttattagaat caagagccgt agcgaggtcg atatcggcac ctgtttttgc ctgaatgagg
     6421 tgatcgcaat gcaccgtaga tggaacggct acttttttct tgccggcact cataaattgc
     6481 agtaatgcca tctgtgcagt agcatcctgc atggcaacgc gatccggtcc aaagtcgaca
     6541 taatcttttc cgcgctcata agctttggac gccatgcctt cgctgaggtg agcgtacaat
     6601 accttttcgg tgagcgtaag tggtcggtta aggagttttc gtgcggcttc aatacgtgca
     6661 ggataagctg catatacttg cctgatcatg tctaagtcaa atgccatatc ttgatgattt
     6721 ttgttatcgg ttttggaggc gtaaaaccct taaaacaggg cattttttgc ctttcgcctg
     6781 caaaggtaac aatcattgcc gattttcgtt tattaaacga ggataaattt ttttttagac
     6841 gagtttaaaa atttcacaaa ccttcatgca accatttagg aggaaaacac attatctttg
     6901 atataaaccc cacctcatga atcgctatac tatcacggcc tattctgcta ctgccggcat
     6961 attcctgctc cattcgaatg ttacagacgc acaaatggtg catgtgaatg tggatccgga
     7021 tgctcacgtt ggaccttatt ttgactataa tctggatttg aatgcggatg ggattaatga
     7081 ggtgtttttt aattgtggcg aattctggac aggctcctct tttggagggg aatttcatgg
     7141 ttattcatct caatggattt atttatatcc tacagggaca tttgcaacat ctatcggaca
     7201 aattaagcac cttgtggtag gagataccgt tgatgcagac atgttattta atgatgttga
     7261 tccgcttttc ttttatggac aaagagatat tatagcaggc ggaggatggg tagacattgt
     7321 tgaaggatct gaatgggtta ataatgaagg gtttttcgga ttgaaatttc caattgacgg
     7381 tgaaacacat tatggatgga ttcgggtgga attaattcaa ttagatcccg attatttacc
     7441 gcaaatgtat gttaaggaat ttgcctacaa tgatacaccg gatgcgccgg ctccaataac
     7501 tatttacaat gcagatattg ccaaatcatt agtgctgagc gatgtcggag aaactaatac
     7561 acctgaagat ctgcaactta cttttaataa agccgacaat gaaataaccg tcagcgaata
     7621 cagagtggca ctttttaaag gagcaattcc tccaacagtt gctcaaatag acagtttaac
     7681 gcccgacaga tatatttctg tatttccgac aggagatgat ttatcggtat catttagtgc
     7741 cgacatattg gacataaatg gcgataccat aattccaagt catttataca gggcgattgt
     7801 attatcagtt gcagatggta cttatgccat tgaaagtgat ctatctgtgc catctaatta
     7861 tacttatttt tcattggatg tagcacctgc aacatatgaa gttgaactgc ttaccgatag
     7921 tattgctacc gatatttccg ggtttatagg atcatttgga atgatggata ccagtgtttc
     7981 tgctgttcgg ttatatatca cggccaatga aatgaatgtt gaagaacttt tggcactcga
     8041 cgaaccatat tatatggaaa caattcctgt tgttggaatt aatactgtgc attttacatc
     8101 ggataaatta atttatgagg caggagaccc tgcattattt aatttgtact atttacatgt
     8161 ggtctcaata cctgatagta ttacaaattc aattccggcg ctctctgttc aaagcggaag
     8221 atttctctat aatcaatatc ctgttgctcc tgagatcacc ataattggat atgcaggtaa
     8281 ctcgagtgat atcctgcttg aattcccaca actccccgac gaggctagtt tagaagtgta
     8341 tcgtgttttt gtttgtaaag ccggaaccgt tgtgaactca gaacttgttg ccggtatgct
     8401 ccccattcat tatgaatctg tatatccatc gggagtcgac ctgcatatgg atctgacagc
     8461 ttgtaatctg gattccgacg gagatgaaat aattgaaggt cagccatatg ttgcctatgt
     8521 agccatgcgc tgcacttgcg atgattattt ttatagtatt accatcccat catcagaatt
     8581 tattttgggt gaaccggaag tagggattga taatatggca acagatgaat taatcataac
     8641 tgacaacgtc atcctgatcc caaatggttt ggaaagttca acattgcaaa tttctaatgc
     8701 atctggacaa atgatgttcg aatatatgat tcaatccggt gatagagata taaaacttcc
     8761 tccattcccg gcgggtatat acatcgccta tatcaagaat gaacagcata tttatgccaa
     8821 gaaatttgtg attacgtcct ccggtggata ataatgtgtt acgtaccttg caacacaaaa
     8881 ctttaatagt gaacactcgc caaggtgaaa aataaatcat cctacatgaa aacacttacc
     8941 ctctccgcct attctgcagc atcagcgctg ttacttggtc tttccaaaga tacaagtgcg
     9001 caaatgatct atcaggacct ggatcctgac tttggtattg gtccggattt tccggtcgac
     9061 ttgaatgatg atggtatttc ggaaatgcgt attcatggac acggttggtg gacgttcacc
     9121 agtgatatgg tgtattcctc tcgacagatc gagtttttta attatgctga gattgccaca
     9181 tctgccggtg tgcttcagat gtttgagggt gatacggttg atgctgatct ggattttgga
     9241 acagcagatc aatttttctt ttacggttat aatgacgacg gttggataga tattgatgag
     9301 ggtgcatcct gggtcaataa taatagttat atcggtatgc gttttgatgt gagtggcgag
     9361 attcattatg gttggttgcg tgttgaattg catcaggatg atattcatga tatgccaaca
     9421 ctttatgtaa aggaacttgc atggaacgca acggcggatg ccccggcacc tatcaatgcc
     9481 catgttggtg tagcgctatt tccggaatta tctgatgtag gtgaaacaaa tactgctacc
     9541 gatctgcaac tccattttca aaaggcagat gatgaaagtc acatcagtag ttatagggtg
     9601 atcttgtatc ccggatttcc tgctccaaca cttgcagaag caaatgcact aagtgccgac
     9661 agatatacgg agttgattcc tgttggtgca gatatcacca tgaattttac agaaactacc
     9721 agagatattg atggcaattt attgcttccc ggaacttttt atagcgcaat aatcttatct
     9781 gttgctgatg gcattgcagt cactgaaaat gacctctctt cgcactcaaa taacatgcaa
     9841 tttatttatt tggaagcacc tgtgcctctg gatgtatatt tgcaatcaac aggttacgac
     9901 gatgatatta ccggattata tggcaggttt tatttgacag acaccgctgt ggcttcagtg
     9961 agagcttatt tatctgatag ttatttcgag gctgatgaat tgttgctgct tgaccctgag
    10021 tattatattg aaacaatacc tgatgcagta ggtactcatt acatgtggtt cacagctgat
    10081 aaacatatct acacctccga tgcacctatt ttatttgagg attatgtgct cagtatagtt
    10141 acagtgccaa atggtatcac taattcaata cctgaactcg gtaccgatgt agctcatttt
    10201 acatatccta attacgatgt catgcctacg gtcacacttg ttgattcaac cggtaccggc
    10261 gcagatattc aattacatta tcctatgttc atcaacgaag atgacttgga attgtatcgc
    10321 attttcatag cgaaggcaga cgtaggtatt gaacctgctt atgcatatac agtcccgccc
    10381 tctaaacgat tagatatttc ccctgcaggc atcgacctgg acgtgaattt gtcctccatt
    10441 acccttgata ctgatggcaa tagtattaaa tataaccaag cctatcgggt atatgttgcc
    10501 ttgaagggcg acttctatcc tgattattat tcacttgcta ccccttcagc agaatttatt
    10561 ttggaggaac ctgttattga atcaattttt tcccaaaata ataaagcagc tccgaatatt
    10621 tgggtgaatg accatttaat acatattgca ggcgcgacaa atgaaatgca tacattgacc
    10681 ttgcttaacg catccggaca agaggtctac agaacaactt tcaacggtac tgaggttgca
    10741 gtagaagcac ctgagctacc cgcaggagtt tacgtagttg gggtggaaag cgagcatggt
    10801 gtgcatcacc aaactatctt tcttcaggga aagtgagtgg aattgaggtt gttgctgaaa
    10861 tttccatggc attttaacat tttaacacat gcaggttcta cctttgtgga aagggtatcg
    10921 gcgtgacatt aagggaaaac atatcattag ccttcagagc catcaaagga aaccgcctgc
    10981 gaagtgtgat caccatcact attattgccc tcggattaac tgccctgatt ggtattctaa
    11041 cggcaattga tgcactagag agttctatca actcgaattt tgccatgttg ggtgctaatt
    11101 cattttctat ccgcaacttc aatgaactga gcaatggaaa tgagaaacca aatccaccca
    11161 tcagttataa ggaagctact tcatttaaag atcgctatac ttattcgggt gccgatgtat
    11221 ctatttcggc cattttcatt caagggtctg cagaagtaaa acaagcagct aaaaaaacaa
    11281 acccgaatgt tgatgtctat ggtgttgatg agaattttat tccggttgtc ggatatacta
    11341 ttaaagaggg aaggggtttc agcaatatcg aattaaattc cgccacacgt gttgtggtgc
    11401 tgggtagtga tgttgctgaa aaattgtttg gcgaaagaga taatcctatc gataaaggtg
    11461 ttaccatcaa cgatcagtat ttcagagtga tcggaattct ggaaccaaaa ggaagcagct
    11521 ttatgcaaag cgataatcgc gtgttgattc cgcttacagc tgcaagaagt attttccctc
    11581 aggtgggtag agattcttac attttatctg tcggtgttaa aactgctgtc gacatggaac
    11641 ccgccatcgg tgaagcaacc ggtctgatgc gtcaagtgcg ccgtttgcga ttgggtgagg
    11701 atgatgattt tagcattgcg aaaagtgata gtgtggtgag tattatgctt gatagtcttt
    11761 cttatgtggc actgggtgca ttatttattg gatttatcac attggttggt gctgctatcg
    11821 gattaatgaa tatcatgctc gtacaagtaa acgaacgcac cagagaaatt ggtgtgagca
    11881 tggccattgg tgcaaataga aaagtcattc gccgccagtt tcttattgag agtattgtaa
    11941 tttgtatcct cggcggtata ttcggaatta ttcttggcat tgcaattggc aatcttctgg
    12001 ctgttgcact gcatgcaaac ttcatattac catggttatg gattattttg ggattgacag
    12061 tttgtgtaat caccggactt gcagcaggat atatcccggc ccgcagagct tccaggttgg
    12121 atcctattga ggcgctgagg tatgagtagg tgagtggtga gtggtgaatg gtgagttgag
    12181 actcgtgaat gtgattcctc cgcaaactcc gcgattgaaa aaaggtgagt ggtgagtggt
    12241 gaatggtgag tggtgagtgg tgagtggtga gtggtgagtg gtgaatggtg agttgagact
    12301 cgtgaatgtg attcctccgc aaactccgca aactccgcaa actccgcgat tgaaaaatgg
    12361 tgagtggtga gtaaatgcat atgcgcatcg tatacccgat cctaaagtac gcaccacact
    12421 cacgaatcac gaatcacgat tcacgcttca cgacccacga gtcacgcttc acgatttcat
    12481 acaattattg ggtcatttgg cattgtgatt ttatcatttt gctcaaggtg cgttgtgaag
    12541 ccttctttga atcattaatt ttggattgta aatttatcat catgatgttg cacaccaatt
    12601 ccaaattact ccaatacaca acgctagctt caacagttct gattggagaa aaaattacag
    12661 cacaatctgt atacgttgat atagatcccg atgtgccatt atttgaagat gtatggagtg
    12721 atgaaggatg gaaatatttt gatttgaacg aagatgggat tcttgatgtg ggcttgagct
    12781 accacgtagg atttatttgc ggttattgtc catatgttat tggtttcgat gtggcattat
    12841 tgaacggagc agaaattgca acaatgattg cgccgtcatt atcactttgg agtgtatacc
    12901 aatcatcata ttcctcgtct gtaggagcat gtcaaattcc atctcatgca atagccgatg
    12961 gtttggctga aggagaagtg ataaatatgg ataatcaatt tggagtaatt gaggagctat
    13021 ttcagacttt tcgatgcggt tcatatggcg atttcggagt gcagaatgat ttagatttcc
    13081 aagatcataa ttggagcact tattctaagc cttatattgg atttcaactg actgttcctc
    13141 ttgggtatca gtatgcatgg ttacgattgt atgaaggcga tgattataat atttatgtaa
    13201 ctgattttgg ataccaatca catgtgaatg caggcctcat gattgaacaa ccggtaacag
    13261 acattgatga atcaaaacct caatcgccat atttgtttag cagtaacgga gttgtgcatg
    13321 tgcaggatgg aaccggctat ctgatgacag tgacagatat gcagggaaag gaaattgttg
    13381 aagagaatat tacatcctca aactttgaga agcgatatga tttacaaatg ggaatttatc
    13441 tgataacaat tgtcaagggt gattcccgat ttacagaaat gttgatgctc aacaattaac
    13501 ataaaaaaag gccatcccga aggacagcct cttttactgt atattctcca acctttattc
    13561 ttcaactact tcaaaggata ccggtacgat atgatctttg tgtaacatta catttgcact
    13621 gaatgttccc aatgttttca cttcgtcggc gatagtgatc tttttgcgat caataaccaa
    13681 tccggtttgt tttttgatag cttctgccaa ttgcacgttg gtaattgaac caaagatttt
    13741 atcggtttga ccaactttag caccaacgcg gatatttccg ttttggaggg tggcaataac
    13801 ttcttgtatt cttgctacga gtttgcgttc acgtgcttct tccgctttct gacgttcagt
    13861 catcattttg cggttacctt cattggcaat aacagccagg ccgcgcggga tcaagaagtt
    13921 acgtccgtaa ccgtcgcgca cttttaccag ggtattcgca tcacccaggt tgtctacgtc
    13981 ttttaagagt atcacttcca tggctatatt attttaaaag gtcagcaaca taaggcatga
    14041 gggccaattg gcgactgcgt ttgatcgcct gagcaacttt gcgctgatat ttcaggctgt
    14101 taccggtgag gcgacgtggc aggatcttgc cttgttcatt aacaaatttc aacaggaact
    14161 cagggttctt gaagtcgata tacttgattc cgctcttctt gaaacggcag tatttcttgc
    14221 gcatggcacc caacttaggg gctgcgaggt aacgaatgtt cttacttgtt gccatgatta
    14281 agctccctcc tcttgaagtt tacctgattc ttttttcgca tttgccttcc ttccgatctc
    14341 tccgcgacgc ttgcggtcgt tgtaatccat agaatacttg tccaaacgca ccgtcagata
    14401 acgcaatacg ttctcatcgc ggttgaattg gatgtcaagc tttccggtta catcggttgg
    14461 tgctttccat tcgagcacaa agtaaatacc ggtgctcttt ttcttaatct tgtaggccag
    14521 ttgcctgagg ccccagcgct cttcggcaac tatttcagct ccgttcgaac gaaggaaatc
    14581 cttgtaggat tcctcggtcc tcttcacgtc gtcatctgag aggacaggag tgaaaatgat
    14641 gactgtctcg tactggttca ttttcgttgt tattaatttt tttagaaatt ggactgcaaa
    14701 gatagcaaat acccccataa tatccaaaaa agccaaagcg ttaaaccctc agccattaac
    14761 tttgtctaaa cctccaaatc ttagatatat gcaccaaatc cgtgcttcca tcaccctcat
    14821 ccccgtactg gcggttgccg tactggccgg atgtaaaaaa gaaagtctcg atactgatac
    14881 ctacattgcc ctcgacaacg ccattgccga cgggtctttt caggacatga tgaatgttgt
    14941 cacccagcaa gcggtagcca acgggatcga cggattcagc tccggcactg ccaatggtaa
    15001 actactggaa gattgtccta caaccacttt cagcgaaccc ctcggcacct tcccgaacac
    15061 tatgaccgtt gatttcgggg tgagctgcac ggcaggatac ctgggcgttg agcgctccgg
    15121 taaagtgatt gcaaccttca caggaccgta ttccgctgaa ggaactacca tcaccatcac
    15181 aactgacaat tactatgtaa acggcaataa ggttgaaggc acaaaaacca tcaccaatga
    15241 gggcctcaac gatgcgggta acctcgaatt tgttgttgta atggtagatg gcgtggtaac
    15301 ccttgccacc ggagaaacta tttcctggaa cagctcacgc acccgcgagt ggatagcagg
    15361 ggacgagacc ttggaaatag aagatgatgt ttattcggta actgatggcc cgggagttga
    15421 caatgctgtt gaaggcatta atcgctccgg cacacccttt acagcgcata ttgccgagcc
    15481 attggttcga gccctcgatt gccgatggtt ccgctccggc gtgcttgaag taacacctga
    15541 agaaatgctt acccgttcta ttgattttgg cagtggtgat tgcgacaatg ttgctacact
    15601 ttctattggc gaatttgaaa ctgaaatcac gctaccgtat taattcttaa agcctgaaac
    15661 cataaaaaaa aaggctgtcg tgataaacat gacagccttt tttattattt cggactaatc
    15721 tcagtcgcta ctacctgtcg ggcagtttac ctgagcatca aattgtttta catattcatt
    15781 attaggatcc aatgttttgg ctttattaaa attagtgcag gcatcggcag tttgtttcat
    15841 cagatcgtaa tatgagaaac aatataaata ggcatcaata aggtttttct tatttttttc
    15901 gatatccttt tcagctaatt caatatacct gaggtaaagt ggcaatgctg ttccgcgttc
    15961 atcaataccg tcgagacgaa ctttcgtttt cgcacgctgt aaaaatccat cgggtgaatt
    16021 tggttgcagg gcaatgaatt gtgcatagat ggtatctgca tttacataat cattggtctg
    16081 ataatatgcg cgaccaagga aatagtaatc caatgctcct gcctgatatt tagcaatcac
    16141 attattgtat gcgacaattg actcggcata ttttttaatg gtataaagat atttcgcata
    16201 ttcagctgct atctcatacg ttgatgtaga atcactgccg gtagcaagtg tataatagaa
    16261 tatagccttg gcagtatctt tagcttcgac agctaattgt gcagagtatt gataatcaag
    16321 tgtcaccaat tttttctcac cgatactcca gaatttttgt gaagcttggt atccatcggc
    16381 aaaatgtttc gctgatgcat caggttctgt tttgcggata gaatcaccta attcataatg
    16441 tgaaaaataa agaatacgcc atgcaacaaa attggtgctg tcaatagcaa taatctgtgt
    16501 tgcagattca atagcacatg cgtaatcctt taattgataa catgtaactg caggtaccag
    16561 gcgagcttct ttatcttcgg cattgagttt tacatattcg tcgaagtatt tttttacgtc
    16621 ggtatagtaa cctgccaaat agctctcttc accaagttct ttatatgcta tggcaagatt
    16681 tggatcaagg cggatggctt ctttatatgc agcaatggca gcatcgtgct tacctgtttt
    16741 agagttaata cgggctatgc gcaagaatgt aacagcaata tttgggttta ctgcttttgc
    16801 ctgttcatat ttggtcattg cttcgccggg tttatcctga ctcagatagg catcgccata
    16861 ggcaagcaag ttgatcacca gattaggatc agttgaatat gcctgatcat agtagctgat
    16921 tgcttcagtc acattaccgg caaccatata ggcatcgccg atcaggtgta tgatctcggc
    16981 attttttcct ttactggctt taatggcaga ttcaaactgt gcctgagctt cggtagcatt
    17041 gttgttaagc aacaacattt taccacgtga aacatagatc aatggatttt tagaatccgt
    17101 aacctcactc gctgctttgt aataatactt ggcagagtcg gtatttccgg tagtaagata
    17161 tacttctgca agatagaaat tgctttctac gctcggtgct gttttattca tattcagcaa
    17221 catgcgtttt gctttgaagt aaaattcaga ttcaatagca tgtttggcat cactgatagt
    17281 ttgtccaaat gctacaccct gtatcaacag gcacactaac aacagtttaa tgatcttcat
    17341 tttaggtcgg tcttattgat tgttattcta ctatattaat tcgcctttcg ggcattttct
    17401 ccggaacaag tccggccttt aacattatgc ggcttgcctt gctcctatat aagaagttga
    17461 caaatcccgt gcctaataat tcaggtgtgt ttcctttaac cactgtaaca taacgctgga
    17521 aaggataatc attggatgca atagaggatt gtgacatggg aattacaatc tttttgcccg
    17581 ttgaatcggc ctttgctacc ggaagcacct ttacctgcct taatattgct tgtgttaacg
    17641 agtcatcagg atcgctgagc gctgcaaagg agataaatcc gatggcagcg gtgtttttgg
    17701 atacataacc cactacagtg tccaaattgc ctagggaaaa catattgttg taaccggtca
    17761 tattacccaa caattgtgat accatggcgc tattttcctc attgaaaaca aagcccgttt
    17821 tctcatatac accggaatag ccggcagaac gactctgcaa aaatgcatca aaatcaaata
    17881 cggtgtcagg atttgacaaa gaagcgatca ccgcaatggc ttctctacct atcacatatt
    17941 gctctgctgc tacctcattt acgctcttga tccggtctaa ttgctccttc gaaagtgcgg
    18001 ttccggtaat gatcatgcgc gttttatcgg tgaacatagc atccaaaact gcattgtcag
    18061 gggcgaatcg gacctcgatt ttcgactcag gatagacatt ttcatacgat ttcaccagtt
    18121 cctccaccag ggtttggtag ctgctttcgg catatagggt gatagtgcct ttattgaggt
    18181 tttcagtcct ttcgtcccag gctttgtctt tacaagcccc gagaaatagg gaaaatgtga
    18241 tgatcagcag aaggttaagc ttgttcatca ttgtcaggtt tattgatttc agataactct
    18301 gtcaccaccc ggtacaagcg gtaaaagcca taaacgatta acaatgcagc aaacaggttt
    18361 accagtaggg gagtgatccc ttccagcatg ttgctcagca tatccggaac gaggagaacc
    18421 agtattccca ttgttacgta cataactgaa gccacgagtt tgagcagcag caccgggtgt
    18481 cgaaagatac tattacgcat cgggtataaa aataaaacca tccttggact tgccgcagat
    18541 ggttttatcg gaataagaat tatttattaa ttgtcttcca aagtaaactg gataggaagc
    18601 ttgtaccaaa cggcagccgg cttaccattt tgtttacccg gagtccattt tggcagcgag
    18661 cgaaccacgc gcattgcttc ctcatcgcaa ccggcgccaa taccgcgcag gagctgaaca
    18721 tcagaaacaa caccgttttc gtcaacaatg aatttcagga acacttttcc ctgaaccccg
    18781 ttgtcgcggg ccattcttgg atattgaata tgcgagttga tgtaattata caatgcttcg
    18841 gtaccccccg gatattcagg catttgttcg atgaattcat ataccttttt ctcctgaact
    18901 acaggtttgt caacaactac cggagctgtt tcgataacga tgtctgcatc ttcatctcct
    18961 tcctgtgttt gggtagagat ttcagcagtg atctcttctt gagtaattgt ctcatcctct
    19021 gcaacatcct catcacgctg ggcgaccatt tccacgaacc ttacagtagg acgtaccgga
    19081 ggtggcggag gtgggggtgg tggaggtggt ggtgcagcct catccagtgg aggtggctcg
    19141 gtgagtgtta ctacgcggtt gatggcttcg tcatcgttct tttttgtaag gaagccaaag
    19201 tcaacaaacg taaaaccggt aagcatacca cttacgacaa acatcacaat ggtagctttt
    19261 acggtattgc gtttggtgag ctgacgcagg aaatacgcac cgtacgattt gttgcgattg
    19321 tcgaagacaa tctcatccat cgattggttt aaatattttt tgttgtcttc catcatagcg
    19381 gaatgtttac tgttaagatg cgtcaggaat aaaattccat aaacatcggg aaattttttt
    19441 tattgtaatc cttcgggaat cacatccagc ttaccgccat tggcaatagc ttcgagttcg
    19501 agatcggcaa catcctgaat ggcgtagatc ttggtatcag tgatatccat ttcatcgagg
    19561 atatctacca tgttcttgta attcgcgtcc tcagtcattt tgatgattac gatagtcttt
    19621 ttagggtctc cgaatttctg accaacttcc ttctgctttt tatagatcac atcgcggatc
    19681 ccatcggcag agaaatcggt tttttgcata tcgtacacca cgcgaccatc ataataccag
    19741 atcttatctt ccttatccag aataacgttt agaacttgtg attcttttac atcggttttc
    19801 actgtttcat ctttcttcgg catattcagc tccatggcta ccggcttgtt cagcgtggta
    19861 gtgagcatga agaaggagat gagcaggaac gcgagatcca ccataggggt gaagtctaca
    19921 cgggtcgaca ttttcttcga cctgaccttg cctttaccat gtttactgtg actcccttta
    19981 tccggggttt gaatatcagc catagttgta aattatttag caggggtagg cgaggcttca
    20041 actcctccgg ctaccggacc ttctaatgtt gtgatcagat tgaatgtgtg aatattaacc
    20101 ggctcttcgg tcaatatttt gattacttcc tgtattgttt tgatactgga agttttatca
    20161 cccttgatag cgacacgaat gtcatctcgt tttgtgcctg actcagtctt atctatctga
    20221 tctgcccaac gtccggcttg aatccagtcg cgaagctgat tgttggtcga gtcaataggc
    20281 acaccggaaa aatcatgcat ggcattgatt tcctgaggag gcatattgag gagttgatcc
    20341 aactgattga tatcgcttcc aagcatttcg acattcgcaa aattggcaat ttgagcatct
    20401 gtgagcttac tgagtgaagg atatgttttc tcatatcgtt cgataagctg ttgcagggtt
    20461 gccacgcgca ctttagggtt gtctattgaa tagtagacct ttccttcttt atccaccgag
    20521 ataaccattt tgttatccgg taccactgtt tcagactcag aacttggcat gtcgatgatg
    20581 acaggctgtg ccggtcggaa ctttgcagta aggatgaaga acgtcagcag caagaatgaa
    20641 acgtccgtca tcgcggtcat gtccactatc gtgctttgtc gttttacttt aatttttggc
    20701 atcgtagttc cgtttagtaa agcaccttaa aagatgcatt agattatttc tcctgtgcag
    20761 agaatgtctg cgtaatgcta aagcccattt catcaattcc ataggtaagt ttatcaatca
    20821 tacctgtgaa gatgttatag aaaatgatgg cgaaagcaga tgttccgata ccgatcgcag
    20881 tgtttacgag ggcttcagag ataccggcag aaagtgccga tgggtcagga gttccttcag
    20941 atgcaagggc agcgaaggcg cggatcatac ctaataccgt tccgagaagg gcaatcagcg
    21001 ttccgatgga agcgatggta gccaggatag gaaggtgttt ttcgagagca ggcatttcaa
    21061 gtgctgtagc ctcctcaatt tctttagaga tgttgatcac cttttgctca gtggtagcat
    21121 ctgtatctga tttcatttca gcatacttat gcaaaccggc ctgaaccacg tttgccacag
    21181 aacctttttg acgatcgcat tctttcaacg cagcatcaat gtctttgttg gcaaggtgat
    21241 atttcacttt ttgcaggaaa gactggatag agccgttacc ttttgctttg ctgatagtta
    21301 gcaacctttc gaaagcgaaa accaaaactg taaggaaaag tgcgaagagg accggaacga
    21361 taaatcctcc tttgaacacg ataccgagga agttaccttc ttttgggtcg ccgttagctt
    21421 tgaagttgtc ggggtttcca caaacgaatt tgtaaaaaag aataccaatt acaaggttta
    21481 ctgcaataac aataataccg agaccatcca tactggtctt tttagcgggt tttgctgctg
    21541 attgagccat aaatgatttg atttaatttc ctgtaaaact tttaagtgtt ggcaaatata
    21601 gatgaaattt taaaaactca attcaccgaa aatgaaaagc cacgcggctt ttgttatttt
    21661 ttcgttaact gagcttccat cttctctgct gttgaactgc aatgttaaac actttcacag
    21721 aacggattgg tatgtggggc ctttggtttt acatatttct gacaataaac gcctgaaaat
    21781 caacatttac tgcacttttc gtcgaaggtg gaggctgttt agtcgaaatt ccggctccga
    21841 tagctccatt ttcggcactt ttgcatcatg tcgcgcctga aattgtcgcc cggagccttt
    21901 tttgcctccg gaaccggtcg tgtgatactg cacgtcgtgt tctggatact ctatatgaat
    21961 tcacccatct ttatgatgga agttaatacc ttctcaagca acggcatcct gttgtttgag
    22021 gtgatgaacc tattatttat cccttattat tacctgctgg ctaatgttct gattcctaag
    22081 ctgtttaccc taaagcgttc acctcttttt gttgtttcct gggttggttc ttacgtattg
    22141 atggcatact gcgttcagca agtgtctatt catttcctat acacactcac cactgaggcg
    22201 gaaatagcga cttttaaaaa agtggcagaa acatcaccca cctattttcc cgaatttttc
    22261 aggatgatat ttgtgaccat ggtgccgctg agtataatgt tggttaggag atatgcgcgc
    22321 atctcggcaa aacaaacgga actcaaaaga atgaatgccg atctggaatt gaattttctg
    22381 aaatcgcaaa tgaatcccca ttttttattt aatagtctga ataatattta tgcattggca
    22441 ttgcaacaat cagaaaaaac gcccggactc attttgcaac ttagtgatct gatgcgctat
    22501 atgctttacg aatgtaatgc tgcgcaagta gctcttgata aggaattaaa tttcttgaga
    22561 gattatatcg gacttgaaaa agtgcgacat ggagaaaagg ttaaaattga atttaatatc
    22621 aagggagaaa cttcgcaaaa gcaaatagct cccttattac tgctgccctt cgtggaaaat
    22681 gcatttaagc atggcgtaaa tgcgcaactc gggccggcat gggtgtctgt cgattttaat
    22741 gccgaagatc cggaaggagg atgtacattt gtagtgcgca acaataaacc ggtaaagaca
    22801 gaaaaaactt cgtcgcaggc aggaggcata ggtttggaga atgcgcgtaa acgattggat
    22861 ttgttgtatc cgaataaata tttgctgcat gtagataatc acaacgaagt gtatcaggtt
    22921 gaattaaaaa ttatggattt atgaatcaca aattgagatg tattgttgtt gatgatgaac
    22981 cgctggctca acaagtgatt gtaacttata tcggacgtct ctcgcacatg gaattggtgg
    23041 ctacttgcga taatgccatg gaagccatgc aagctttaca aaaagacaag atagatgtaa
    23101 tgtttttaga tattcaaatg cccgtgatta caggcgttga atttttgcgc agtctgcaac
    23161 atccgccgcg cgtaattttc actacagcat atcccgattt tgctttggat ggttatgatt
    23221 tgaatattac ggattatctg cttaagccaa tttcatttga acgatttatg aaggctgtca
    23281 ataaattaag tgaagcggta aacaacgagg aacataatac gccgattccg gatcatttct
    23341 ttgtgaaaga agacgggaaa ctggtgcgcg tgaattttgc tgatattgat catatcgaat
    23401 gcatgaaaga ttatgccaaa atctttacaa agcaacggat gattgttacc catcattcga
    23461 tgaagaaatt tgaagaagtg ctgcctgacc aacagtttat gcggatacat cgcagctata
    23521 tcgtaagcct ttcgtctatt aacgcgattt ttgggaatgt catagaaacc gctaaaggcc
    23581 gcttgccggt aggcgccaac tacagggaga tgcttatgga aaaaatcaag gtcgactaac
    23641 aggggattga ttggaattcc gttactttgt aaaaaactgc aatatgaaga aaatccttac
    23701 cctagttctg gcatttactt ttttctcaca ggcacatgcg caaatcaatg atcatgctat
    23761 aggtatccga tttggctttg gtggtgggat ctcataccag caaaaaatga gcaagacgaa
    23821 ccgactcgaa ttgaacggct ccgcgaattt ttataatggc tactcagcct ttcgattgac
    23881 cggtgtgtac cagtgggttt gggatatgaa taaaggtttc agtttttatg cagggcctgc
    23941 tttgtcgacc ggcacaatga gccttggtga taattataat gggaaaggaa aaaacggatt
    24001 ttttgtcagt ggcggcggtc aagtaggatt agattacaat tttgacggat taccgcttca
    24061 gttttcagtg gatgtgatgc cgctgttccc gattattaat aatgtagatg atttttatct
    24121 cgaccctgca ttgggcgttc gctttgtgtt ctaagcgacg ataataacat tatgtgaggt
    24181 tgtcggcttg atgcacggca accttttttt attacctttt ttctctacgg cgtttgtaaa
    24241 tgcgtatggc aacgagcatg ctgaaggaaa gtgctatcaa ccctaataat ccatacaaca
    24301 tatccacgag gtctttcaat acaacaagaa aaacgatggc tactaataac actgtagcta
    24361 cttcattgaa catacgcagt tttgtagagc tatttttagc tattccttct cgcaattgtt
    24421 tttgtatgcg aagacatgcc agatgataaa ctataacgcc aatcacaaaa agcagtttgg
    24481 tatgcaacca tggttctttc aagaattgtg attgaatgct gagcaatgac acaccggtaa
    24541 ttacaacaag gatcatggat ggcgtgccga taatagccat tagtcgttgc tccataatgg
    24601 taaactgtgc tgccaaaatt gagcgttccg gctcttgctt ttcgagcgct tcagtatgat
    24661 agatatataa tcttacaata taaaacagtg cagcgaacca tgtaatcaca aagatgatat
    24721 gtgctgctaa aaaatatgga taatattgtt gcatggttat cgtactagcg ttatgttgcc
    24781 ccgttcttca cgttttttat tattcaactt aacttgcatg acatatacat aagttccgat
    24841 aggttgtaat tcattttcaa attttccatt ccaaccttga gcaatttcgt cggtttcgaa
    24901 aataagttgg ccgtatcggt tataaatttc taaatgataa tcttttatat cacccgaata
    24961 atttgggcct atccaatcat ttatgccatc attatttggt gaaaaaacgg ttggcattac
    25021 cacatggt
//
LOCUS       DAOQHY010000026        25008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_870_length_25008_cov_5.319962, whole genome shotgun sequence.
ACCESSION   DAOQHY010000026 DAOQHY010000000
VERSION     DAOQHY010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 25008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25008
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_870_length_25008_cov_5.319962"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..1581
                     /locus_tag="PLL28_03705"
     CDS             106..1581
                     /locus_tag="PLL28_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68453.1"
                     /translation="MVKNIFITFLVCIALADLQAQTYLHPTSGIDGTYVGACMVNTCS
                     GNYYDDGGAASNYSNNISSYYRVFCPNTAGNCITATFTSFDVQNDCTKDYLTIGNGAT
                     QNSTTIAIPGVTTVAGGANRICGSPPVPFSATATNASGCLVMRFTSNASSNAAGWAAT
                     ISCSACAIGPVATDPNDCINAIAICDDLPVTGVESGPGLVSESCSGCTAGGENYSTWY
                     YFCFETSGTFGFTIDPVTNTDDYDFALYGPDVSCGALGTPVRCSYAASTGNTGLKATV
                     SDVSETVTGNGWVDTLHVTAGECYYLMVNHWSPPVTGYTIDWDLTDGATFVDCDLLPV
                     TLTSFYAEAYDNNTVSLTWETETEFNNDYYIIERSTDGQTFEPFMQLDAKGNSSDKTT
                     YFAVDAHPNLGINYYKLSQVDLNGRTTYLKTASVDVQYNNSFGTISIYDLSGRIIHSE
                     VVYRSQIAVMLDAIQLDPGVYVYRYLQENGVSENGKFSVVR"
     gene            1772..2587
                     /locus_tag="PLL28_03710"
     CDS             1772..2587
                     /locus_tag="PLL28_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68454.1"
                     /translation="MQRSILCITAMTIFTTWACSCSDKDLNERKAISDQLTTVAIENT
                     LLLIRQTIGFERQIESEALDRFKSQNYFKMLAVDSAISSIIYGIKTYNQQFDKTTLRR
                     HKDRVMRIANLDEYEEKYVSENMLSTYDTLGDFSFPNEAILHMLQFEFYVQKIMSRRS
                     SYCGNIDNGHLTKLDEHTYAVSIQWNDHRLYFMDSMDVNYGWINYHFIRRDQMKVYHG
                     GTYVLITFESPTPFKEEFIYAGIAYKRKNGVTGYLDDQYYALFTNVVNEDKNP"
     gene            2628..3431
                     /locus_tag="PLL28_03715"
     CDS             2628..3431
                     /locus_tag="PLL28_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68455.1"
                     /translation="MKRYIHAIYTSCIIYCMGCSTPEHNNKDELIRSSYAVVEAYNNM
                     LNRNAIYKLQNDSREESQLILNTLETMYPQAGYSVPSRFISSDYFSQLDSLVDQEPYT
                     SLPMKNLREAIVKWRYDLHQMKRPLSLDEARLMRAMLDNLLFDYANRIIQSDCFWGLR
                     EIPYVANNGNIIITDPHSFNDQVMRNAVIKNVQVLINDENISPVAFRHSTTLNPLIFE
                     IDKHAVELQEENVFRINYTIENVMFKSGSEQQIWQTLTSSEYYNQIQWK"
     gene            3428..4225
                     /locus_tag="PLL28_03720"
     CDS             3428..4225
                     /locus_tag="PLL28_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68456.1"
                     /translation="MRTATLLLMASLFVCALSCKQVERPEHMTSTQILAMQAIDATEN
                     QISQWIGITRYHSQRSSHAGLQDIYDNMLSISKASDNAIDGINSGKLSFRQDSLSLIK
                     RNIIRSQSYSNESRRYWRDSMVYSFDTLPVFSCKEEAILSIKLLELMMIRDLGRSYTV
                     CTEYEDAPLFWIDDHTVVAILGSKAINMFTKLDTIHIISGWIANNPLTPEQYHVQISG
                     NYCLITIAVPDDYKQPFIEAGFEYVFNAEDRAYSRRCYASFAGYKEK"
     gene            4323..5225
                     /locus_tag="PLL28_03725"
     CDS             4323..5225
                     /locus_tag="PLL28_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNF68457.1"
                     /translation="MKRFYSTLITCICITVIQAQQVHVSSGKVIRHANFPSQFVPPRN
                     VDVWLPDNYDATKSYAVLYMHDGQMLFDATTTWNKQEWQVDEHMGKLLQSGEIRDCIV
                     VGIWNVPHLRHSEYFPAKAVPYIEKTLIDSLKKVEWESALPTSDAYLSFIVRELKPFI
                     DNNYATKTDKANTFIGGSSMGGLISLYAICEYPAVFGGAICMSTHWQGSLKVLDDNIS
                     TGMLKYYRKHLPNPATHKIYFDYGSETLDKAYKPFQLIMNKAMNKRGYTFEHWMTREV
                     PGADHSELAWSARLEIPLVFLLAE"
     gene            5406..6893
                     /locus_tag="PLL28_03730"
     CDS             5406..6893
                     /locus_tag="PLL28_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68458.1"
                     /translation="MYFSFQLLHQLTADERKLVENASLTSRERELMDFMLTQHSKHFP
                     AVKALHALKISRTNLYKLNSNLLTNLLEIIAPDNILVKLEYLMNRSSVYALRSQLLKQ
                     HHEKIKHSATQADHTFYREFYKWICSDNSSDTIKELQSEITAMVIATSKPEAEKEVKL
                     FIDLNAFRVEMFKKWRNGTIENNSKFKELKERGYSIINSARQYGDKIIIRSSYDSCIF
                     MFISNKEFEDAKSLLEEFNILIKSEPETYSDISNWYVGSMKGVIEYTQLNMDLAYSYF
                     SKHLYLTLDHPIHSQNNIYARYFITTLLLNKSEIAREIYNKWNMQFSFDPRISLYTNH
                     LQIKTMFEIFNEKYDSVIATLNILELNGWYNYGFSLQYFLREYRLSIAYLTDDFGTAH
                     ALAKKNLTYIRYSKRNKIQPEVAFHSKFVKVLIKCIRKKRQLQKSEFGMLVKLIEYHP
                     LHALTLQRCFNKYQVLFNNEPLVVWEPEKGISDLVLEYIKMPEPA"
     gene            complement(7024..7224)
                     /locus_tag="PLL28_03735"
     CDS             complement(7024..7224)
                     /locus_tag="PLL28_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68459.1"
                     /translation="MKPKMKEKRVQSRIRQIANLSAMRPPDADLELPPAELLKKRRVI
                     YFWRDMHNMALFINMSRGDSAE"
     gene            complement(7326..11417)
                     /locus_tag="PLL28_03740"
     CDS             complement(7326..11417)
                     /locus_tag="PLL28_03740"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68460.1"
                     /translation="MGKFRHLVWLMLLTSALHAQPGMLGTQAVNGSYTSYSLNDMGIF
                     RQMRVQATSSAASGTRNWEFYEAAADYDPAWRPYFSAMTLSGYNQPIAPSGASASATY
                     NLGFGGASGLLPAVTSGNYYTFNVTEKSTPGTPLDEHMGVMETSYNPVAITSVTQSPG
                     IGAVYPENSVYVTVTLASTLHADEYVYVRYSTTFNFTSSSLLPVTISGTTGTVEIPCQ
                     AASTTIYYYAYTSNRTSAEILADVAVPAIGEYAHDMSTLCINNNGGPNYSYTVLPSIG
                     FCGSYYIPSTCYPTINSFVTALNAGTVGCSVICYVAAGHTETAPAGGINLTQTGTAVK
                     TITFIKNGAGANPIIYAPVGTVSIVAASTVADGVFSLNGSDYITIDGIDIIDNNTTGA
                     TMMEYGYALFKASNTDGCQNNTIKNCAITLNNNNSWTGAPLNFEFGATGIMMRNTART
                     ALTTLFTITGVSGRSDNNQFYGNTITNVINGFVLSGYNDVTSPYTYYDQNNYVGVGGL
                     GNTIEKYGNTTATVRSSGVYAIYNNALVVSNNTIGNYLSGGTAHNNTLYGVFVSSPIS
                     GNNLQTLNIQNNTISLKGHTGSSRVTGIKVGNASCGATSITIDGNTIEDCTFTAGSTG
                     EFYGIDIGFNATAVAITNNIIQNNVINTTTTSVQYLIYNNTTGSAIDVSYNQMLNNYK
                     TVSSTAGILYGYYSASGSATGTQVFHDNIIDGLGIPSAQNGSVCAVRISSSINQSKEI
                     YNNAVSNITSGTSIATMYSCGYYIDGMNVGATVHDNSLSDVTCGNYFAGFNFCSAAST
                     ASTANVSYSCYNNTISNVTTTSTNTGPHGIAAYMPTTGSPVINIYNNNITGLNSTASF
                     SGYIYGIFMGGGSANATLNFYENTLHDFVHTGAATNTIVAGGLIALGGSTVNFYRNSI
                     NHITNTGSNGISYGLYITSTGVVNIYNNFIQRLSSTATTNVIGVYGIYTGSSGGFWQF
                     VNNTIALGYDTPLTSTGANFGFTGIYFAPTGVTATVLNNIVYVRGTANGTALGSCIRG
                     SIGTAYTPPASFSLSNNNYYFINNATYNYIYTEGGFNTSNVNGYAYGGAFTDVTRNLN
                     NDDCFNDPATGLYKAFMSPAESNSYYDVPPFAGGGSYPYNLKLTTGATTYAESNASAI
                     PGITIDYEGDARLGSTPDIGADEGDFVPQAVDCFLLPIELMSFSGEISGDDNLLIWTT
                     ATEHNTSYHIVERSVTGFDFTAIGSVPAAGESESEITYNFVDQHPGSGIRYYRIRTVD
                     LDESNSLSNVIALDRELANSTQTLIFPNPVHDILTVMHDWQTTEGVQIVVLNELGQAV
                     LTKDFAAGDKITVDVTALPGAVYFIRLIDLNSEIIYSGTFVKE"
     gene            complement(11542..13179)
                     /locus_tag="PLL28_03745"
     CDS             complement(11542..13179)
                     /locus_tag="PLL28_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68461.1"
                     /translation="MRTFLPAVFLFFTITVSAQLQLPLQANRVSALFSANGFLFTDFS
                     YMAQYTVPGYSAGQENNATIFAGGFWMGGYDDADQLHLAASTYRQTGDDYWAGPIATN
                     YTNPDYIDRYSKVWPVDRAEIENHIANWDVPGYLVPPSIAYWPGNGNVANGEAAILAP
                     FYDYNNNLIYDPENGDYPLVRGDKAIYMIFNDAAEEHGESGGEALGVELHVMAYGFDM
                     PEDSALNQTVFVNVSVINRSVNNYNEFIFGNFADFDLGAYDDDYVGCDSNLNVFFAYN
                     GDPVDGPTFGNYGSNPPAQGVIFLNRPMHAFKYYNNDFSVTGNPTTGEDFYNYLNGKW
                     IDGTNQTEGGNGYGGAVPMNFMYPGHPMDPDAWTEMREENVPSDRRGVGSIRPVTLNA
                     GDSMCIEMAYLSAFVFDTVNAEGTTTDVHALGATEILLRRTVDVIDYYNANYEACQIA
                     YQSNPNYSSIVASDIANNISISPNPANDFIEVQLGGENVIGTIMLMDNQGRRIYESTL
                     SGQPSISLEITEYPAGMYNLLIIPESGAILSASFIKH"
     gene            13499..14947
                     /gene="gatB"
                     /locus_tag="PLL28_03750"
     CDS             13499..14947
                     /gene="gatB"
                     /locus_tag="PLL28_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNF68462.1"
                     /translation="MEVYDKYEAVIGLEVHAQLLTRTKAYSSDIAAYGGEPNSYVSPV
                     TLGHPGTLPVFNAQSLNLAMRLGLALHCQITRYNEFARKNYFYVDLPKGYQITQDKTP
                     ICTGGHVDIGAYGQERIIRLTRIHMEEDAGKSIHDLDPFYTLVDLNRAGTPLVEIVSE
                     PDLRSGDEAYAYLTEIRRLVRYLDICDGNMEEGSLRCDANVSVRLKGAAEFGVKVEVK
                     NMNSIRNVKRAIDYEIKRQIDAIEAGETIVQETRSFDAAKGSTFSMRSKEAAHDYRYF
                     PEPDLQPFIVSDAHIADVKANMPALPRDLMHKYVEQCKLSVYDAEILTAERDIALYFE
                     ELIALTNNFKAAANWMQGPVKSWLNEHAENIEALPIKPAQIAELIALTESGKLNFTIA
                     SQQLFPAMLENAEIAAEALAGKLNLIQQSDAGEIEKLVEEAISKYPEKVAEYRSGKKG
                     ILGLFMGEVMKLSKGKADPKLATRLLEEKLNA"
     gene            15006..16142
                     /locus_tag="PLL28_03755"
     CDS             15006..16142
                     /locus_tag="PLL28_03755"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNF68463.1"
                     /translation="MKKLSLILLPLLIGGLLNSCASQKSEAFEISGVIKGAENKKVLL
                     ESMTFPANGQPNFVVIDTAWTDNIGNFEIKNYLPERMICRMTIDGDQMNYYILSVHNE
                     KIKIDASWKDPGNPKVEGSSATNSLFGLMDALRSFDTEAGMMNDSLRTLKSLGKDSIV
                     DAILDDMKLNYFAIFKNYVDTTTVTSNAVLALESMYDFDFDYVKSYYNKAKNSADSSS
                     VYLKEMGEKITLREAELANSIVGKPFMDVEQPDPKGNMRKLSDLKGKVVLLDFWASWC
                     GPCRQENPNVVKAYNKYKSKGFTVFSVSLDDNKNKWLDAIKKDGLIWENHVCLLDQKN
                     NKAASDYRVTGIPMSFLIDQNGIVIAENLRGGALEKKLAELLPD"
     gene            complement(16379..17470)
                     /locus_tag="PLL28_03760"
     CDS             complement(16379..17470)
                     /locus_tag="PLL28_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HNF68464.1"
                     /translation="MANSKRKTSLIGDKEIDFLRSYINNPSPTGHEAAGQKIWLNYIK
                     PFVDDHIVDNYGSVAAIINPTAPFRVVIEAHADEIGWAVQYITEDGFIYVGRIGGSDH
                     IIAPSKRVNIHTASGVVKGVFGWPAIHTRMGKEETPDMKNIFLDLGCTSKKDIEKMGV
                     RIGDPITFDDDFFVMNNYYVGRAIDNRIGGFMIAQVARMIKEQKVKLPFALYIVNSVQ
                     EEVGLRGAEMMAQTIKPHVAIVTDVTHDSNTPMISKTLEGDIKVGRGPVLTKGPSVHN
                     KLLQLMQDTADANKIPYQRDFASRSTGTDTDAFAYSNGGVPTSLIALPLRYMHTTVES
                     VHKDDVENIIKLMFYVLKSLKPNFNFKYL"
     gene            complement(17489..19120)
                     /locus_tag="PLL28_03765"
     CDS             complement(17489..19120)
                     /locus_tag="PLL28_03765"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNF68465.1"
                     /translation="MFPKTIGDNGHYYRFVWLRFSIFRAETEMPVEDKIKRLHGMQAE
                     ALLGGGAERIASQHKKGKLTARERVHLLMDEGTFEEIGQLVVHRSTDFGMEKQQFYGD
                     GVVTGYGKVNGRLVYVYAQDFTVFGGSLSETHAEKICRIMDLAMQNGAPVIGLNDSGG
                     ARIQEGVNSLGGYADLFYRNTLASGVIPQISAIMGPCAGGAVYSPAITDFILMVERTS
                     YMFVTGPNVVKTVTHEEVSSEDLGGAVTHGSKSGVSHFTAANGLDCIQKIKRLLSYIP
                     QNCEEESPVYPYTMGDELRPALDRIMPENPNHPYDIREVITEVADVDSFFEVHETYAE
                     NIVVGFARVAGKSIGIVANQPAVLAGVLDIEASRKAARFVRFCDCFNIPLVVFEDVPG
                     FLPGTDQEWRGIITNGAKLLFAFSEATVPRITVITRKAYGGAYDVMNSKHIGADMNFA
                     WPSAEIAVMGPKGAAEIIFKKEIEDAADRDAKLQEKIDEYTETFANPYRAAFRGFVDE
                     VIKPSETRIRIIRSLDMLKNKVAVLPRKKHGNIPL"
     gene            19142..21667
                     /locus_tag="PLL28_03770"
     CDS             19142..21667
                     /locus_tag="PLL28_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HNF68466.1"
                     /translation="MAYGTANRMVPKAEILTFAIEMMTRLFAVFAFLLMLTSVQQLEA
                     QIPGITISGTLLDSTNAKPLEYGTITIKHPGDSTIITGVLSDEAGKFKIQVATPGKYE
                     LQLYYVGYKRKILPLDITPGNPFTDLGKITLAADGGLLDVVEVTAERGYFQGSIDKKV
                     YNIEKDIIASNGSASDALQTIPSVVIDIDGNISLRGSEGVRIFIDGKPSGIAGSNMNA
                     ILEQIPANTIESIEVVTNPSARYDAEGNSGIINIVLKKNKKIGLNGNITAGVSTSPRY
                     EAGASLNYRNKKVNIYSNYSFTHDDRDGESYTYRKTFEEDTISYLLSNGSSNNISNMH
                     MARAGIDYYLTDHTTLGVSGSWHGNEGSNTGLVDYSFLNIDSTLTSTSKRTTSGTNNG
                     IGWNGGATIRHTFKDPKHVWSAEAYYANGSSDDSTYYAEDFYDATPAFWYSLVPQNIS
                     RPNENIDITAQSDYVRPFKNGNQFETGIKYTQEKKDNTFYSESFYSIGADPASWNADD
                     SLNNQFIYNEDVIAGYLIWNGTFKKKFGYQIGLRAEQTYTLSQLITTDEDFTHDYFGL
                     FPSAHVRYAVDETMEFSASYSRRIDRPNAWFLNPFRDYSDPYSFRQGNPFLDPEYENS
                     YEIGFEKEFKKHSLTASLYYTKTLNEISPYITVTPEGVSYMTFQNYNDESKYGFELVT
                     RDEFFKWWNMTGSFNFNQTLVNAENLEAGLTNNQFTYNIRLMHMFAVYKQTSLQLTMS
                     YNTPWTFAQGESNAIYFMDAGVKSDFFQNKLSVNVNFSDIFNSRKWSGVSEGTNFYSE
                     YSRKRQSQILSLKLTYKFGQQDNSRRRGNRGMDENYDGGVDMF"
     gene            complement(21671..22675)
                     /gene="hemH"
                     /locus_tag="PLL28_03775"
     CDS             complement(21671..22675)
                     /gene="hemH"
                     /locus_tag="PLL28_03775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNF68467.1"
                     /translation="MKKKGILLVNLGTPDSPATADVRSYLKQFLLDPRVIDIPAIPRN
                     LLVRGIIAPFRAPKSAALYKKIWTDEGSPLLVYSKKLHQKIATALGDNYSVVLAMRYK
                     NPSIESGLQLLANQGCTHITVVPLFPQYASATNGSVVEHVMAIVSKWQSIPDLHFTGP
                     FYDHPKFIQAITQIASTYQLQDYDHFLFSYHGVPERQIHKAANGNACLAEGCCDVMHK
                     DNALCYRAQCFQTTRLIASALNLPTDKITTCFQSRLGKTPWIQPYASETITGLAAEGK
                     KKLLVFSPAFVADCLETIYEIGVEYDTEFRHLGGEKVQLVESLNDSDLFVECLVDMVK
                     "
     gene            complement(22743..>25008)
                     /locus_tag="PLL28_03780"
     CDS             complement(22743..>25008)
                     /locus_tag="PLL28_03780"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNF68468.1"
                     /translation="INGAYWGVYELRNKLDEGHFDHVKGIEKDSLDLMTLSYWYGLVL
                     RTLSGSDTDFIEMRNYLGTYPTPADDDFYDIADSILDLNNFTDYIICQTWFANYDWPY
                     NNIKAWRDRGGDNKWRYAIIDVELGLGIGGWSDANANLIPGLFYTQQYIEPLATLLQN
                     PKYREYFINRYADLMNSTYLTDRTLSMEDSIYNEIYPELPRQLERWGYGAVEDQLATF
                     EDYRNALRNDFSIRTNKVRLHIKDGFALENKVDVTLNATPAGCGHIQISTLDIYDFPW
                     TGVYFNGNEVQVTAVPLPGCTFDHWEANPFITDIYNPTFKVNIDAYTTTFTAVFTGTP
                     YEQKIAVSELNYHPESSLDDGNWIEIVNAGADPVSLDGWYIRDSEPYHRYDIPAGIEL
                     NSGDRWVFADDTAKFHLLHPDVSNVSGPLGFGIGDYGEDMLLINARNEIVEHMHYDDA
                     APWPLGADGHGRTLERNDMYADVNDPLNWFDGCIGGSPGLAYSPCEDPIVFSEINYNS
                     NAALDAGDWIELHNVTDEPLHIGEWIFMDDSIGTEHQFMIPSGTTLEPHAHLVLAQDL
                     ALFNSAHPGVTNVIGGFPFTFSGNGEWLRLYDQSEVLSFAVDYQDITPWPTVADGAGY
                     TLELVDSLGVMNDGNNWTAYCLGGSPGTFVIEPCPETIAINQISDIQSQISVYPNPVN
                     QYALTAISIEYPVNASIRLFDAQGGLRGTLFDGILQAGSFAISLDMSAYPAGMYFLQL
                     QSDNKQSTIAVIKQ"
BASE COUNT         7736 a         5220 c         4947 g         7105 t
ORIGIN      
        1 tttaaaatca caatttatag tgtaaataaa gcaattgaaa attatactta tcgtttcaaa
       61 aaagacgaag aagatcaata atttcctctc ctcacaccct cacacatggt aaaaaatatt
      121 tttataacat tcttggtatg tattgcactt gccgatttac aagcgcaaac atatttacat
      181 ccaacctcgg gtatcgatgg aacttatgtc ggtgcttgta tggtaaatac ctgctccgga
      241 aattattacg acgacggagg ggctgcaagt aactatagta ataatatcag ttcttactac
      301 agagtatttt gccctaatac tgccggcaat tgcatcacag caacatttac cagttttgat
      361 gttcaaaatg attgcacaaa agattatctc accataggaa acggtgctac acaaaatagt
      421 actacaattg ccattcctgg agttacaaca gttgcaggtg gagcaaatag aatttgtggc
      481 tcgccacctg tgcctttttc cgctactgcc accaatgcca gcggttgtct ggtaatgcgc
      541 tttacctcca atgcatcatc aaatgctgcc ggatgggcgg caactattag ttgttctgcc
      601 tgcgcaatcg gcccggtagc aacagatcct aatgactgta taaatgccat tgcaatttgc
      661 gatgatcttc cggtaacagg cgttgaaagt ggtcccggac tagtttctga atcatgcagt
      721 ggttgcactg ccggaggtga aaattattca acctggtact atttctgttt tgaaacttca
      781 ggcacttttg gatttactat cgaccctgta accaataccg atgattacga ttttgctttg
      841 tacggacctg atgtatcatg cggtgcttta ggtacaccgg ttcgatgttc ctatgccgca
      901 agtacgggaa acaccggact taaagctacc gtttcagatg tttcagaaac agttacaggc
      961 aacggctggg tagacacact tcacgttact gccggcgaat gttactattt gatggtaaat
     1021 cactggtcgc caccggtaac gggttatacc atagattggg atcttactga tggcgctact
     1081 tttgttgatt gcgatttatt gccggttacc ctcacctcat tctatgcaga agcttacgat
     1141 aataatactg tttcattgac ctgggaaaca gagaccgaat tcaacaatga ttattatata
     1201 attgagcgtt caactgacgg tcaaacattt gaaccattta tgcaattaga tgcaaaaggc
     1261 aattcatcag ataaaaccac ttatttcgct gttgacgcac atcccaattt aggcatcaat
     1321 tactacaagc tttctcaagt ggacttgaac gggagaacta cctatctcaa gactgcatct
     1381 gttgatgttc aatacaacaa ctccttcgga acgataagca tttatgattt atcaggaagg
     1441 attattcatt ctgaagtagt ttaccgaagc cagatagcag taatgctcga tgccattcaa
     1501 ttggatcccg gcgtatatgt gtatcgctat ttgcaggaaa atggtgtgtc tgaaaacggg
     1561 aaattctctg ttgtaagata aatctccctc tattgaatac ttattgatta tacgattact
     1621 ttaaagtaat tcgtttacat ggtaattacc caaaacgctg cggtcaattg ccgcacgtgc
     1681 tgtgttcgtt tctgcgattt aaggctaaat cagacttcct aaaaaacgcc attttaggaa
     1741 ggaattttca catttgtgca aacagacctt catgcagaga tctattttat gtatcaccgc
     1801 aatgaccatc tttactacat gggcatgcag ctgcagtgat aaggatttaa atgaaagaaa
     1861 agcgatcagc gatcaactta caacagttgc gattgaaaat acattattat tgattcgtca
     1921 aacaatcggt tttgagcgac agatagaaag cgaagctctc gaccgattca aatcgcagaa
     1981 ctatttcaaa atgctcgcag tggatagtgc catctcatcg ataatttatg ggatcaaaac
     2041 ttataaccaa caattcgata aaactacatt acggcgtcat aaagatcgtg taatgcgcat
     2101 tgccaatctt gacgagtatg aggaaaaata tgtgtcggaa aatatgctat caacttatga
     2161 cacacttggc gatttcagct ttcctaatga agccattctc catatgcttc agtttgaatt
     2221 ctatgttcaa aaaatcatgt caaggcgcag tagttattgc gggaatatcg acaatggaca
     2281 tttaacaaag ttagatgagc atacctatgc agtttctata caatggaatg accatcgatt
     2341 atatttcatg gattcgatgg atgtgaatta cgggtggatc aattaccatt tcatccgacg
     2401 cgatcagatg aaggtttatc atggcggcac ctacgtattg atcacattcg aaagccctac
     2461 acctttcaaa gaagaattta tatatgccgg aattgcatac aaacgcaaaa atggggtaac
     2521 aggttattta gatgaccaat actatgcctt atttacaaat gttgtcaatg aagacaaaaa
     2581 cccataacat aaaaataaac tcattgtata ataactacaa attcaaaatg aaaagataca
     2641 tacatgctat ttatacttca tgcataattt attgcatggg ttgttctacg cctgaacata
     2701 acaacaagga tgaattaata cgttcatcat acgcagtcgt tgaagcatac aacaatatgt
     2761 taaatcgaaa tgcgatatac aagcttcaaa atgattcacg ggaagaaagt caattgattc
     2821 taaacaccct ggagactatg tatccacagg ctggttactc cgtaccttcc aggttcatca
     2881 gtagcgatta tttcagtcaa ctagattcac ttgtagatca agaaccctat acgtcattac
     2941 caatgaagaa ccttcgcgaa gctatcgtaa aatggcgata cgaccttcac caaatgaagc
     3001 gaccacttag tcttgacgaa gcaagattaa tgcgcgcgat gcttgacaat ttactatttg
     3061 attatgcaaa tagaatcatt caatcggact gtttttgggg attgagagaa ataccttacg
     3121 tggcaaacaa tggtaatatt ataattacag atccgcattc ctttaatgat caggtaatgc
     3181 gaaatgctgt tatcaaaaat gtacaggttc taatcaacga tgaaaatatt tcacctgttg
     3241 cattccgaca tagcacaacg ctaaatcccc tgatctttga aattgacaag catgcagttg
     3301 aattgcagga agaaaatgtt ttcaggatca actatacaat agaaaacgta atgttcaaat
     3361 ccggatcaga acagcagatt tggcaaacgc ttacatcaag tgaatattac aatcaaatac
     3421 aatggaaatg agaaccgcta cattattatt gatggcatca ctgtttgttt gtgccctgag
     3481 ttgcaaacag gtagaacggc ctgaacatat gaccagcacg caaattttgg caatgcaggc
     3541 aatagatgcc accgagaacc agatatcaca atggataggc ataacgcgat atcattcaca
     3601 gcgaagcagt catgcgggat tgcaagatat ctacgacaat atgttatcaa tttccaaggc
     3661 atctgataat gcaatagatg gaattaattc aggcaagctt tccttccggc aagattccct
     3721 atcacttata aaacgaaata tcatcagatc acaatcttat tctaacgaat ccagacgtta
     3781 ttggagagat tcgatggtat acagcttcga caccctgcct gtattttctt gtaaggagga
     3841 agcgatcctg agcataaaat tattggaatt gatgatgatc agagaccttg gaagaagtta
     3901 caccgtttgc acagaatatg aagacgcccc cttattctgg atcgatgatc acactgtagt
     3961 agcaatactt ggtagcaagg ctataaatat gttcacaaag cttgatacca ttcatatcat
     4021 atcaggatgg attgccaaca atcccttaac acccgaacaa taccatgtcc aaatctccgg
     4081 caattattgc ctgattacaa tcgctgttcc cgacgactat aaacaaccct tcatagaagc
     4141 cggttttgaa tacgtattca acgcggaaga tcgagcttat agtcgcaggt gctacgcatc
     4201 ctttgcaggc tacaaagaaa aataggtata ctttgcacta agcttttcaa ttcctatttt
     4261 tacaacagga gcatcgtttg cccgtaaaat gaccggctag atttatgctc ctaccactca
     4321 ttatgaaacg cttttattca acgctcatca catgtatatg cattactgtg attcaagcac
     4381 aacaggttca tgtttcatct ggaaaagtaa tcagacatgc caatttccca tcacaatttg
     4441 tgccaccacg caatgtagat gtttggctgc cggataatta cgatgcaaca aagtcgtacg
     4501 cggtattata catgcacgac gggcaaatgc ttttcgatgc caccacaaca tggaataagc
     4561 aagaatggca ggttgacgag cacatgggga aactgcttca atcgggtgaa atccgcgatt
     4621 gcattgttgt tggcatttgg aatgttccgc atttacgaca ttcggagtac tttcctgcaa
     4681 aggcagttcc ctatatcgaa aaaacactaa tcgacagtct caaaaaggtg gaatgggaat
     4741 cagccttgcc aacttcagat gcatatttat cattcatcgt acgcgagctc aaaccattta
     4801 tcgataataa ttacgctaca aaaacagaca aagcaaatac ctttatcggc gggtcaagca
     4861 tgggaggact gatttcactc tacgccatct gcgaatatcc ggctgttttc ggaggtgcga
     4921 tttgtatgtc aacacattgg caagggagct taaaggtgct cgatgacaat atttctaccg
     4981 gcatgttgaa atattaccgc aaacacctac caaatcccgc tacacataaa atttactttg
     5041 actatggctc agagacattg gataaagcct ataagccctt ccaactcatt atgaacaagg
     5101 ctatgaacaa acgaggttat actttcgaac attggatgac aagagaggtg ccgggtgccg
     5161 accatagtga attagcctgg agtgcgcgtt tagaaatacc attggtgttt ctgcttgcag
     5221 aatgacgtct aatcttgttt tgttccctca ggtcccattt ttcatgattt tagcgcaata
     5281 tcttacgcat gacgtaagga ttacactaaa atattttgaa aaatcacgtt aatgttaatt
     5341 ttggagcagc aactatatta gagggagcaa tatagcttgc catacaaaaa cggggattta
     5401 attatatgta cttcagtttc caattgcttc atcaactcac tgctgatgaa cgaaaacttg
     5461 tagaaaacgc atcgcttacc tcacgcgaac gcgagttaat ggacttcatg ttaacccaac
     5521 attcaaagca tttcccggct gtcaaggctt tacatgcgct aaaaatatcc cgcaccaatt
     5581 tatataaatt aaattccaac ttacttacaa acctactgga aattatagct ccggacaaca
     5641 ttctggtaaa gttagaatac ctgatgaatc gcagtagtgt atatgctttg agatcgcagt
     5701 tgctgaaaca gcatcatgaa aaaataaagc attctgcaac acaagcagat cacacctttt
     5761 acagagagtt ttataaatgg atatgcagtg ataattcaag tgatacgatc aaagaattac
     5821 aatcggaaat cactgccatg gtcatagcca catccaaacc cgaagcggaa aaagaggtaa
     5881 agctattcat tgatttaaat gcctttcgtg tagaaatgtt caaaaaatgg cgtaatggta
     5941 ctattgaaaa caattcaaaa tttaaagagt taaaagaacg aggttattcc ataatcaatt
     6001 cagcaaggca atatggcgat aaaattataa taagatcgtc ttacgacagt tgcattttta
     6061 tgtttatttc aaacaaggaa tttgaagatg ccaaatcatt acttgaagag ttcaacatcc
     6121 taataaaatc agagcctgaa acttattcag atattagcaa ttggtatgtg ggatcgatga
     6181 aaggggtgat tgaatacact caattaaata tggatttggc gtatagctat ttttcgaaac
     6241 acctttacct tacactggat caccctattc attcccaaaa caatatctat gcgcgctatt
     6301 ttatcacgac actattatta aacaaatcgg agatagcccg cgaaatctat aacaaatgga
     6361 acatgcaatt cagttttgat ccaaggatat cgctatatac gaatcatttg caaatcaaaa
     6421 caatgtttga aatctttaat gaaaagtatg attcagttat agcaacatta aatattttag
     6481 aattaaatgg atggtataat tacggattta gtttacagta ttttttgagg gaatatcgat
     6541 tgtctatcgc ctatctcact gacgactttg gaacagctca tgcacttgct aaaaagaatc
     6601 taacctatat caggtactct aaaagaaata aaatacaacc tgaagtcgca ttccactcta
     6661 aattcgtaaa agtgctgata aagtgcattc gcaaaaaaag gcaattacaa aaatctgaat
     6721 ttggaatgct tgttaaactt atagaatacc acccccttca cgccttgact ctccaacgat
     6781 gttttaataa atatcaagtg ctttttaata acgaaccatt agttgtttgg gagcccgaaa
     6841 aaggcatttc agatcttgta ttggaataca taaaaatgcc tgagccggca taacaccatc
     6901 tcaggcaaat tagtcaatct aacatgacag aattacgctc ctggctcgac tgactttatg
     6961 aaaaaacacg acttataatg catcccgttt actagaacta atagaagtat atttcactaa
     7021 ctcttattcc gctgaatcgc cacgtgacat attgataaac aatgccatgt tatgcatgtc
     7081 acgccagaag tatatcacac gtctcttctt taacagctca gcaggcggca actccaaatc
     7141 agcatcagga ggtcgcattg ccgacaaatt cgcaatttga cgaattcgac tttgcacacg
     7201 tttttctttc atctttggct tcattgcatt caattataac ccaaagaaat gcaataggcg
     7261 aagaaataaa ttgcaataaa atcacaaaat cttacgtaaa gggaatcaat actacttcag
     7321 ttggtttact ctttaacaaa agtccctgag tatatgatct cactgtttag gtcaataagg
     7381 cgtatgaaat aaacggcacc aggtagcgct gtcacatcaa ctgttatttt gtcgcccgca
     7441 gcaaaatcct ttgttaaaac tgcctggccc aattcattta gcacaacaat ttgaacaccc
     7501 tctgtggtct gccaatcatg cattactgtt agaatatcat gcacaggatt tgggaatatc
     7561 aaggtctgag tgctattagc aagctcccga tccagcgcaa tcacattact caacgaatta
     7621 ctctcgtcaa ggtcaacggt tctgatgcga tagtatctga ttccgctgcc gggatgctga
     7681 tcaacaaaat tgtaggtgat ttcagattcc gattcacctg ctgcaggaac agagccgatt
     7741 gccgtgaaat caaaacctgt aactgatctc tcaacgatat ggtaactagt attatgctca
     7801 gtagctgtag tccagataag caggttatca tcgcctgaaa tttcgccgct aaatgacatt
     7861 aactcaatag gcaatagaaa acaatcaaca gcctgaggaa caaaatctcc ttcatcggca
     7921 ccgatatccg gtgtagaacc taaacgtgca tcgccctcgt aatcaatggt tattcccggg
     7981 attgcagatg cattcgattc ggcataagta gttgcaccgg tggtaagctt taaattataa
     8041 ggataactgc cccctcctgc gaatggtggc acatcataat aactattgct ttctgccggc
     8101 gacataaatg ctttataaag cccggtggcc ggatcgttaa aacaatcgtc gttattcaaa
     8161 ttcctggtta catcagtaaa agccccgcca tatgcatatc catttacatt agatgtgttg
     8221 aatccgcctt ctgtatatat gtaattatag gttgcattat taatgaaata ataattatta
     8281 ttacttaatg aaaatgaagc cggcggtgta taagcggtgc caatgcttcc gcgaatacaa
     8341 gatcccaggg cggtgccatt agctgttcca cgtacataaa caatattatt caaaacagtg
     8401 gcagtaacgc cggtaggggc aaaatagatt ccggtaaatc cgaaattggc gccggttgat
     8461 gttaatggtg tgtcataacc cagcgctatg gtattattca caaattgcca aaacccaccg
     8521 ctggaaccgg tataaatgcc atacacccct atcacattag tagttgcagt ggatgacagt
     8581 cgctgaataa aattattata aatatttact acacccgtac ttgtaatata taaaccataa
     8641 gagatgccat tgctacctgt atttgtaata tgatttatgc tgttgcgata gaaattcacc
     8701 gtagaaccac ccaatgcaat tagcccgcct gccactatcg tattggtagc agcaccggta
     8761 tgcacaaaat catggagcgt attctcataa aaattcaagg tagcatttgc agaacctcct
     8821 cccataaata ttccataaat atatcccgaa aaggaggccg tagaatttaa gccggtaata
     8881 ttattgttgt aaatatttat caccggcgaa ccagttgtgg gcatataagc agcaatacca
     8941 tgcggcccgg tatttgtgct tgttgttgta acattcgata tggtattatt ataacaagaa
     9001 tacgaaacat tagcggtaga tgcggtggat gccgcactgc aaaaattaaa cccggcgaaa
     9061 taattgccac aggttacatc cgacaagcta ttatcatgca ccgtggcgcc tacattcatg
     9121 ccatctatgt aatagccaca actatacata gttgcaatac ttgttccgga ggttatattg
     9181 ctaacggcgt tattataaat ttccttgctt tgatttatgg atgaggaaat ccgcaccgca
     9241 catacacttc cattttgtgc agaagggatg cctaaaccat ctatgatatt atcatgaaaa
     9301 acctgagtac cggtggcaga tccggaagct gaataatagc cataaagaat accggccgtg
     9361 ctggataccg ttttataatt attcagcatt tgattatatg aaacatctat tgccgaaccg
     9421 gtagtattat tataaatcaa atattgaacg cttgtggtag tagtgttgat tacattgttt
     9481 tgaatgatat tgtttgtaat agctacagca gttgcattga atccgatatc aattccataa
     9541 aactcacctg ttgatccggc agtgaatgtg caatcttcga tagtatttcc atcaattgtt
     9601 atagatgttg ccccacagga tgcattacca acctttatcc ccgtaactct gcttgaaccg
     9661 gtatgtcctt tcagtgaaat agtgttgttt tgaatattta atgtttgaag gttatttcca
     9721 cttattgggc ttgaaacaaa cacaccatat aaagtgttat tatgtgcagt tccacctgat
     9781 aagtaattac caattgtatt gttggataca accagcgcat tgttgtaaat tgcatataca
     9841 cctgatgacc tcactgtagc cgttgtatta ccatactttt cgatagtatt acccagcccc
     9901 cctactccaa catagttatt ttgatcataa taggtatatg gtgacgtaac atcattgtat
     9961 cctgataata caaatccgtt tataacattt gttattgtat ttccataaaa ctggttatta
    10021 tcacttcttc cggacacacc tgtaatggta aataatgtcg ttaatgctgt tcgcgcagta
    10081 ttacgcatca taatacccgt agcaccaaat tcaaaattca gcggagcgcc tgtccatgaa
    10141 ttgttattgt ttaaagtgat agcacaattt ttaatcgtat tattttgaca accatcagta
    10201 tttgatgctt tgaataatgc atatccatat tccatcatgg tagcacctgt agtattatta
    10261 tcaattatat ctatgccatc tatggtgata taatcactgc cattcaaact aaacacacca
    10321 tctgcaacag tggaagcggc aactatactc accgtaccta ccggtgcata aataatcgga
    10381 ttcgcgcccg caccgttttt tatgaatgtg atggtcttaa ctgctgttcc tgtctgggta
    10441 agattaattc cacctgcagg tgctgtttct gtgtgtcctg cagcaacata gcatatcaca
    10501 gaacaaccaa cggttcctgc atttaatgcg gtaacaaagc tattaatggt tggataacag
    10561 gtgctaggaa tataataaga accgcagaaa ccgatacttg gtaaaaccgt ataactgtaa
    10621 tttggacctc cgttattatt gatgcaaagg gtgctcatat catgtgcata ttctccaata
    10681 gcaggaacag caacatcagc caaaatttct gccgaagttc tattggaggt atacgcataa
    10741 taataaatag ttgtagatgc cgcctgacat ggaatttcaa ctgttcctgt ggtgcctgat
    10801 atggtaacag gtaataatga ggaagaagtg aaattaaatg tcgttgaata tcttacatat
    10861 acatattcat ctgcatgcaa tgtcgatgcc aatgtaacag tgacataaac agaattttcc
    10921 ggataaactg cgcctattcc cggtgattgc gtaacagagg taattgccac cggattatag
    10981 gatgtttcca tcacccccat atgttcatct aaaggcgttc cgggcgtaga tttttccgtt
    11041 acgttgaaag tgtaataatt tccggatgta acggcaggca gtaatccgct tgctccacca
    11101 aatccgagat tataagttgc agatgccgaa gcgcccgagg gtgcaattgg ctgattgtat
    11161 cccgatagtg tcattgcaga aaaatacggt cgccatgcag gatcgtaatc tgctgctgct
    11221 tcataaaatt cccagttacg tgttccggag gcagcagacg aagttgcctg cacgcgcatt
    11281 tggcgaaaga ttcccatatc gttcaggctg tacgaggtgt aactgccgtt caccgcctgc
    11341 gttcctagca tgccgggttg agcatgcaat gcagaggtga gcagcatcag ccatacgaga
    11401 tgtcggaact tgcccatgtt acaaatttac aaatagctta ccaaacgtct atggcaatag
    11461 ccgtgaatta tgtgcaaaag cccgacatca ttgagatacc gggcatttct actgcaacaa
    11521 accaccaaaa aagcaagcct gttaatgctt aataaaggat gccgataaaa tagccccact
    11581 ttcgggaata atgagcaagt tatacatccc tgcaggatac tcagttattt caaggcttat
    11641 tgatggctgc cctgaaagcg tactttcata gatccttcgt ccttggttat ccatgagcat
    11701 aatggtgccg ataacatttt ctccacccaa ttgcacttct atgaaatcat tggcgggatt
    11761 tggactgatt gatatattat ttgcaatatc actcgcaact atcgaagaat aattcggatt
    11821 cgactgatag gcaatctgac atgcttcata attagcattg taataatcaa ttacatcaac
    11881 agttcttctc agtaatattt ccgttgcacc taatgcatgc acatcagtag ttgttccttc
    11941 agcattgacg gtatcaaaaa caaaagcaga aagatatgcc atctcgatac acatactatc
    12001 accggcattt aatgttaccg gtcttatcga ccccaccccc cgcctgtcgg aaggcacatt
    12061 ttcttcccgc atttctgtcc acgcatcagg atccatcgga tgtccgggat acatgaaatt
    12121 cattggcacc gctccgccat aaccgtttcc accttcggtt tgattcgtgc cgtctatcca
    12181 tttaccattg aggtaattat aaaaatcttc tcctgttgtc ggatttccgg ttacactgaa
    12241 atcgttgtta taatatttga aagcgtgcat gggtcgattg agaaaaatga caccctgagc
    12301 aggtggattt gatccataat ttccaaatgt cggcccatca accggatcgc cattgtaggc
    12361 aaaaaataca ttcaaatttg aatcacatcc aacataatca tcgtcatatg caccgagatc
    12421 gaaatcagca aaatttccga aaataaattc gttgtaatta ttaactgaac gattgattac
    12481 cgaaacattt acaaatacgg tttgattcaa tgcactgtct tccggcatat caaatccata
    12541 tgccatcacg tgtaattcaa cgcccaaggc ttctccacct gattcaccgt gttcttcggc
    12601 ggcatcattg aaaatcatgt atatagcttt atcgccccgc accaatggat aatctccatt
    12661 ttcagggtca tagattagat tgttgttata gtcataaaaa ggggcaagaa tggcggcttc
    12721 gccgttagca acattcccgt ttccgggcca atacgcaatt gatggtggaa ctaaatatcc
    12781 cggcacgtcc cagttggcaa tatgattttc aatttcagct cgatctacgg gccacacttt
    12841 tgaatatctg tcaatataat ccggatttgt ataatttgtt gcgatagggc ctgcccaata
    12901 atcatcaccg gtttgacgat atgtgctggc agcaagatgg agttgatcgg catcatcata
    12961 accgcccatc caaaatccac cggcaaaaat tgtggcatta ttttcctgac ctgcactata
    13021 tcccggtact gtatactgag ccatatagct gaaatcagta aataaaaatc cattagcaga
    13081 aaagagtgcg ctaacccgat ttgcttgcaa tggcaattgc aattgagccg aaacggtgat
    13141 tgtaaaaaat aaaaatactg caggtaagaa agttctcatg atgtgtaatt tgagaacaaa
    13201 ttaaaaccta ttaatcgagc tgtctgtcat gaatttgttg atattggtac taaattgtca
    13261 gcccaatgta taattcacat ttactgccgg aatgcatatt gattgttgga agatctaggg
    13321 cgatataggt acataaaaaa accggctgcc agctcctctt ccgacaccgg ttagtataca
    13381 tatgaaagaa atccaactgt atccaagtcg aagataacaa aaattttaaa atccaaactt
    13441 tgggtcaaac ttcctcaaac tcttttcagg cggcggtatt gcttatcttt gcgcccttat
    13501 ggaagtgtac gataagtatg aggctgtgat tgggctggag gtgcatgcgc aattgctcac
    13561 ccgcaccaag gcatacagtt ccgatattgc agcatatggc ggagagccca acagttatgt
    13621 gagtccggtt acgcttggtc acccgggaac cctgcctgtt ttcaatgctc agagcctgaa
    13681 tctagccatg cgactgggat tggccttaca ttgccaaatc acccgttaca atgagtttgc
    13741 ccgtaaaaat tacttttatg tcgaccttcc aaaaggttat cagattacgc aagacaagac
    13801 cccaatatgc accggtgggc atgtagacat cggggcctat ggacaggaac gcataatccg
    13861 attgacccgt attcacatgg aggaagacgc cggcaagagc atccatgacc tggatccgtt
    13921 ttacaccctg gttgacctta accgggcagg cacgcctctg gttgagatcg tttctgagcc
    13981 cgatttgcgc agtggtgatg aagcctatgc ctatctcact gagattcgca ggttagtgcg
    14041 ctaccttgat atttgcgatg gcaatatgga ggaaggaagt ttgcgttgcg atgccaacgt
    14101 gagtgtccga ttaaaaggag cagcagaatt tggtgttaag gtagaggtga aaaatatgaa
    14161 ctccatccgc aacgtaaagc gtgcaattga ttatgaaata aagcgacaaa tcgatgctat
    14221 cgaagccggt gaaaccattg tgcaggaaac ccggagtttc gacgccgcaa aaggaagcac
    14281 ctttagtatg cgtagtaaag aagctgcaca tgattatcgc tatttccctg aacccgattt
    14341 acaacctttt atagtaagtg atgcacatat tgccgatgta aaagctaata tgcccgcttt
    14401 gccgcgcgac ctcatgcata aatatgtaga acagtgcaag ttgagcgtgt atgatgcgga
    14461 aattctaact gcagagcgcg atatcgcatt gtattttgag gaattaattg cacttacaaa
    14521 taatttcaaa gcggcagcaa actggatgca gggtccggta aaaagttggt tgaatgagca
    14581 tgcggaaaat attgaagcgc ttccaataaa acctgcgcag atagcggaac tcattgcatt
    14641 gacagaatcc ggaaaattga atttcaccat tgccagtcag caattgtttc ctgctatgct
    14701 tgaaaacgca gaaatagcag cagaagcact tgccggaaaa ctgaatctga tacagcaaag
    14761 tgatgccgga gagatagaga aattggttga agaagctatt tcaaaatacc ctgaaaaagt
    14821 tgctgaatat cgcagtggaa agaaaggcat tttgggatta tttatgggtg aggtaatgaa
    14881 attaagtaaa ggaaaagcag atcccaaact ggcaactcgc ttgttggaag agaaactcaa
    14941 tgcctgaact atttaatatt caatactgtt gaattaatac aaatctgaaa atgattaaca
    15001 taaacatgaa aaaattaagt ttgattttat tgccacttct gatcggtggt ttactgaatt
    15061 cctgtgcatc acaaaaatct gaggccttcg aaataagcgg tgtgatcaaa ggcgcggaaa
    15121 ataaaaaggt attattggaa agcatgacct ttcctgcaaa cggtcagcca aattttgtag
    15181 taatagatac tgcgtggaca gataatatag gaaattttga aataaaaaac tacctgccgg
    15241 agcgcatgat atgccgtatg acaatcgatg gcgatcaaat gaactattac attttatctg
    15301 tacataatga aaaaataaaa atagatgcca gctggaaaga tccgggtaat ccgaaggttg
    15361 aaggtagttc tgctacaaat tcattattcg gattaatgga tgcgcttcgc tcatttgata
    15421 ctgaagcggg tatgatgaat gatagcttgc gcacgctaaa atcacttggc aaagattcta
    15481 ttgtagacgc cattcttgat gatatgaaat tgaactactt cgcaattttc aagaattatg
    15541 tcgatactac taccgtaact tccaatgcag tgcttgcatt agaatctatg tatgatttcg
    15601 acttcgatta tgtgaagtca tactacaaca aagcgaagaa ctctgccgac tcatcttctg
    15661 tatatctcaa ggaaatggga gagaaaataa cactgcgtga agctgaatta gccaatagca
    15721 ttgtaggcaa accttttatg gacgttgaac aacctgaccc aaaaggaaac atgcgcaaat
    15781 tgtcggacct gaagggcaaa gtggtattac tcgatttctg ggctagctgg tgtggccctt
    15841 gcaggcagga aaaccctaat gtggtaaaag catataataa gtacaaatca aagggattca
    15901 ctgttttcag tgtttcactt gacgataata aaaataaatg gcttgatgct atcaagaaag
    15961 atggcctgat atgggaaaac catgtttgtc tgctagatca gaagaacaat aaggcagcat
    16021 cggattatcg tgtgaccgga attccgatgt cgtttctgat agatcagaat ggtattgtga
    16081 tagcagaaaa cttacgtgga ggagcattgg agaaaaagct ggcagaatta cttcctgact
    16141 gacgctcgtc tgttgatata atattgaaag agccttctga aaatggaagg ctttttcttt
    16201 ttctttggag gcaagattcc agctgcccta tcctcccgct ttttcctccg ggcgaataca
    16261 ataaatataa taaccgctgc aataaacgga agaaatacaa atagtagaaa agccgcaaca
    16321 attatctgta tgctcattcc ttaaagcccc tgttagtaac tgttgattac gtaaattctc
    16381 aaaggtattt gaaattgaaa ttcggtttaa gcgatttcaa tacgtagaac atgagtttga
    16441 taatattttc aacatcatcc ttatgcacgg attcaactgt agtatgcata taccgcaatg
    16501 gcagcgcaat caagcttgta ggtactccac cattcgaata ggcaaatgca tctgtgtccg
    16561 tacctgtcga tcttgaggca aaatcgcgtt ggtatggtat tttattggca tctgctgtat
    16621 cctgcatcaa ttgaagcagt ttgttgtgga cagaagggcc ttttgtaagt acaggacctc
    16681 ttccaacctt aatatcacct tcaagcgtct tggagatcat cggtgtattg ctatcatgcg
    16741 ttacatcggt aacgatagct acatgtggct taattgtttg cgccatcatt tctgcaccgc
    16801 gaagtccaac ttcttcttgc acactattta caatatataa agcaaaaggc aatttcacct
    16861 tctgttcttt tatcattctt gctacctgag cgatcataaa tccgccgatg cgattatcta
    16921 ttgcgcggcc cacataataa ttattcatca cgaagaaatc atcatcgaaa gtaataggat
    16981 cgccaatgcg cacgcccatt ttttcaatat cttttttcga tgtacaacca aggtcgagga
    17041 agatattttt catatcaggc gtttcttcct ttcccatgcg tgtatgaata gcaggccaac
    17101 cgaaaacacc tttcaccaca ccggaagcag tatggatatt tactcgttta cttggtgcaa
    17161 taatatgatc gctgccgcca attctaccca cataaataaa accgtcttcg gtaatatatt
    17221 gaacagccca tccgatttca tcagcatgtg cttcgattac tacgcgaaag ggagctgtcg
    17281 gattaataat ggctgcaacg cttccgtaat tatcgacaat atgatcatcg acgaaaggtt
    17341 taatataatt aagccagatt ttttgacctg cagcttcgtg acctgtaggt gaaggattat
    17401 taatatagct gcgcagaaaa tctatttcct tgtcgccaat cagtgatgtt ttgcgtttag
    17461 agttagccat gaataataaa gacttaattt acaatggaat attgccgtgc ttttttcgtg
    17521 gaagcactgc cactttattc ttcaacatat ccaatgagcg gataattcgg atacgtgttt
    17581 ccgaaggttt tatcacctca tccacaaaac cgcggaatgc ggcgcgatat ggatttgcaa
    17641 aggtttcggt atactcatca attttctcct gcaatttcgc atcgcgatct gcagcatctt
    17701 caatttcttt tttgaaaatg atctcagcag caccttttgg tcccataacg gcaatttctg
    17761 ccgatggcca ggcaaaattc atatcggcac caatatgctt gctgttcatt acatcatagg
    17821 cgccaccata tgctttacgt gtaataacgg taatgcgagg tactgttgct tcactgaaag
    17881 caaagagcaa tttcgcacca ttggtaataa tgcctcgcca ttcctgatcg gtgccaggta
    17941 agaatcccgg aacatcttca aaaacaacca atggaatatt aaaacaatcg cagaaacgca
    18001 caaaacgcgc cgcttttcta ctggcttcga tatctaatac accggctaat actgcgggct
    18061 gattagcaac aataccgata ctctttcctg caactctggc aaagccaaca acaatatttt
    18121 cagcatacgt ttcatgcact tcgaaaaagc tgtcgacatc agccacttca gtaatcacct
    18181 cgcgaatatc ataagggtga tttgggtttt cgggcataat acgatccaat gctggacgca
    18241 actcatcacc cattgtgtat ggataaaccg gagattcctc ttcgcaattc tgaggaatgt
    18301 aggatagcag tctttttatt ttctggatac aatcgaggcc atttgcagcg gtgaaatgcg
    18361 atacaccact ttttgaacca tgtgtaacgg ctccaccgag atcttcactg gaaacttctt
    18421 catgtgtaac tgttttcacc acgttaggtc cggttacaaa catatacgag gtgcgttcca
    18481 ccatcaaaat gaagtctgta atggcaggag aataaactgc tccgccggca caggggccca
    18541 tgattgcaga aatctgaggg atcacaccac tggcgagggt attgcggtaa aagagatcgg
    18601 catagccacc aagagagttg acaccttcct ggatgcgcgc tccgccactg tcgttcaatc
    18661 cgataacggg agcgccgttc tgcatggcaa gatccatgat ccgacaaatt ttttcggcat
    18721 gagtctcgct caaactgcct ccaaacaccg taaaatcctg ggcataaaca tacaccaagc
    18781 ggccatttac cttgccatag ccggttacaa caccatcgcc atagaattgt tgtttttcca
    18841 tgccgaagtc ggtactgcgg tgcaccacca attgcccgat ctcctcaaag gtgccttcat
    18901 ccatcaagag atgaacgcgt tcacgcgctg tcagttttcc ttttttatgt tgagatgcaa
    18961 tacgttcggc acctcctcca agcagggctt cagcctgcat accatgtaat cgtttgatct
    19021 tatcctccac gggcatttca gtttcagccc gaaaaatact aaaccttagc catacgaagc
    19081 gataataatg accattatca ccaattgttt tagggaacat ttgaggttaa tttttgttac
    19141 aatggcttat ggcactgcga atcgcatggt tcccaaggcc gaaatcctta cttttgcaat
    19201 tgaaatgatg acaagattat ttgccgtatt tgccttcctc ctcatgctta catcagtaca
    19261 gcaattagaa gcacaaatac ccgggattac tattagcgga acattattgg attcaacaaa
    19321 tgccaaacct cttgagtacg gcacgataac tataaaacat cccggcgatt caaccataat
    19381 taccggagtt ttatccgatg aagcaggaaa attcaaaatt caggtagcta ctccgggcaa
    19441 atatgaattg caattgtatt acgtaggcta taaaaggaag atattacccc tcgatatcac
    19501 acccggaaat ccatttactg atcttggaaa aataacttta gcagctgatg ggggattact
    19561 ggatgtggtt gaagtaacag ctgaaagggg atattttcaa ggcagcatag ataagaaggt
    19621 atataatatt gaaaaggaca tcatcgcttc gaatggttca gcgtcagatg cattgcagac
    19681 cattccgagt gtagtgatag atattgatgg taatatcagt cttaggggta gcgaaggtgt
    19741 acgcattttt attgatggta aaccaagcgg catagcaggt tcaaatatga atgccattct
    19801 ggagcagatt ccggcaaata ccatagagag tattgaagta gtaaccaacc cctctgcacg
    19861 atatgatgct gaaggaaatt ccggtatcat caatattgtg ttgaagaaaa ataaaaaaat
    19921 tgggctgaac ggaaatatta ctgcaggtgt atcaacatca ccgcggtatg aagcaggtgc
    19981 atcgttgaac tatcgcaata aaaaagtgaa tatttattcc aattattcat tcacgcacga
    20041 tgaccgtgat ggagagtcat atacttatcg gaagacattt gaggaagata ccatttctta
    20101 ccttctttca aatggctcaa gtaataatat ttccaatatg cacatggcgc gcgccggaat
    20161 cgattattac ctcacagatc acacaacact aggcgtgagc ggcagttggc atggcaatga
    20221 aggatcgaac accggattag ttgattactc ctttctaaac attgacagca cattaacaag
    20281 caccagtaag cgcacaactt ccggaaccaa taatggcatt ggatggaatg gtggtgctac
    20341 catcaggcat accttcaaag accctaaaca tgtatggtca gctgaagcat attatgccaa
    20401 cggtagtagt gatgattcaa catattatgc cgaagatttt tatgacgcta caccggcctt
    20461 ttggtatagc ctcgtacctc aaaatattag ccgacctaac gagaacattg acatcactgc
    20521 acaatcagat tatgtgcgac catttaagaa tggtaatcag ttcgaaacag gtataaaata
    20581 tacacaagag aaaaaagata acacattcta cagcgaatct ttttatagca ttggtgcaga
    20641 tcctgcaagc tggaatgcag acgattcatt aaataatcaa ttcatataca atgaggatgt
    20701 tattgcaggt tatttgattt ggaatggcac attcaaaaag aaattcggat atcaaattgg
    20761 cttgcgtgcc gaacaaacat ataccttgtc gcaactgatt acaaccgatg aagatttcac
    20821 acatgattat tttggattat ttccaagtgc gcatgttcgc tatgcagttg atgaaactat
    20881 ggaattctcg gcaagttata gtcgacgtat tgacagaccg aatgcctggt ttctaaatcc
    20941 atttcgcgat tattcagatc cttatagttt tcgccagggc aatccatttt tagatcctga
    21001 gtatgaaaac tcgtatgaaa ttggttttga gaaggaattc aaaaaacatt cacttacagc
    21061 atcattgtat tacaccaaaa cattgaatga aatttcacca tatatcacag taactcccga
    21121 aggtgtatca tacatgacct tccagaatta caatgatgaa tcgaaatacg gatttgagct
    21181 ggtaacgcgt gatgagttct ttaagtggtg gaatatgacc ggtagtttca atttcaacca
    21241 aacgcttgtt aacgctgaaa atttagaagc cggacttaca aataaccaat tcacttacaa
    21301 tatccgactc atgcatatgt ttgcggtata taagcaaacc agtttgcaat tgaccatgag
    21361 ttacaataca ccttggacct ttgcgcaagg cgagtcaaat gcaatttatt ttatggatgc
    21421 aggcgttaaa tctgatttct tccaaaataa attaagtgtg aacgtgaatt tcagtgacat
    21481 tttcaattcc agaaaatgga gcggtgtgag cgagggaacg aatttctatt ccgaatattc
    21541 acgcaaacgt caatcgcaaa tattaagtct caagctaact tacaaattcg gccagcagga
    21601 taacagccgt cgacgtggta acagaggaat ggatgagaat tatgatggtg gtgtagatat
    21661 gttctgatga ttatttgacc atatccacca gacattcaac aaacaggtcg ctgtcgttca
    21721 ggctttcaac aagttgcact ttttcaccgc ccaaatgacg gaattccgta tcgtattcga
    21781 cgcctatttc ataaatcgtt tccaggcaat cggcaacaaa ggcaggactg aacactaaca
    21841 atttcttttt gccttccgct gcaagaccgg taattgtttc agaagcgtac ggttgtatcc
    21901 atggagtttt acctaatcta ctttgaaagc aggtagttat cttgtctgta ggcagattta
    21961 gtgccgacgc aataagtctc gttgtttgaa aacattgcgc ccgataacac aaggcattgt
    22021 ctttatgcat gacatcacaa catccttctg ccaaacacgc atttccattt gccgccttgt
    22081 gtatttgtct ttcaggaacg ccatgatatg aaaacaaaaa atgatcgtag tcctgtaatt
    22141 gatatgttga tgctatttgc gtaatcgctt gaataaactt gggatgatca taaaacggac
    22201 cggtaaaatg cagatcagga atgctttgcc acttgctgac aattgccatt acatgctcaa
    22261 caacagaacc atttgtagca gatgcatatt gcggaaataa aggaaccaca gtgatatgag
    22321 tacatccctg gttggcgagc aattgcaatc cgctttctat tgacggattt ttatatcgca
    22381 tggcaagtac taccgaatag ttatcgccga gtgcagttgc aattttttga tgaagttttt
    22441 tgctataaac taataatggt gaaccctcat cggtccatat ttttttatac agcgcagcac
    22501 ttttaggggc acgaaaaggg gcaatgatac cacgcaccaa taaatttcgt ggaatggcgg
    22561 gaatatcgat tactcgcgga tcaagcaaaa attgcttcaa gtagctgcgc acatctgctg
    22621 ttgccggaga atcaggagtg ccaagattta ccaataaaat ccctttcttt ttcatcgtat
    22681 tgccattgca aaggtaaatg gataaacacg aaaaattgaa agaggttcta ttacaagaat
    22741 gattattgct tgatcactgc aatagttgat tgtttattat ctgattgcag ctgcaagaaa
    22801 tacatacccg ccgggtaagc cgacatatct aaagaaattg caaaactacc tgcctgcaag
    22861 ataccgtcaa acagagtgcc acgaagccct ccttgtgcat caaacaacct tattgatgca
    22921 ttaacaggat attctataga aattgctgta agtgcatatt gatttaccgg attagggtac
    22981 accgaaattt gcgattgtat atcactaatt tggtttatgg caatagtttc agggcatggt
    23041 tctattacaa atgtccccgg cgatcctccc aaacaatagg ctgtccagtt attgccatca
    23101 ttcattacac caagtgaatc gaccaattcg agtgtatatc ctgcgccatc agctacagtt
    23161 ggccatggag tgatatcctg ataatcaacg gcaaagctca aaacctcaga ttgatcatat
    23221 aatcttagcc attcgccgtt accgctgaaa gtgaaaggaa atccaccgat cacattcgta
    23281 actccgggat gtgctgaatt gaataatgca agatcctgag caagtacaag gtgtgcatgt
    23341 ggttcaagcg ttgttccgga aggtatcata aattgatgtt cggtgccgat ggagtcatcc
    23401 ataaatatcc attcgccgat atgcaatggt tcatcggtga cattatgcaa ttcaatccaa
    23461 tcgccggcat ctaacgcagc attgctatta taatttattt cgctgaaaac gatagggtct
    23521 tcacatggag aataagctaa gccgggagaa ccgccgatac agccgtcgaa ccaatttagt
    23581 ggatcgttca catcggcata catatcattt cgttcgagtg ttctgccatg accatcggca
    23641 ccaagcggcc agggagcagc atcatcgtaa tgcatatgct caacaatttc gttgcgtgca
    23701 tttatgagca acatatcttc accataatcg cctatgccaa aacctaatgg cccggataca
    23761 ttacttacat cgggatgtaa taaatgaaat ttcgcagtgt catcagcaaa cacccatcta
    23821 tcgccgctat ttaattcaat gcctgcagga atatcataac gatgataggg ttcgctatca
    23881 cgaatatacc aaccatcaag ggaaacagga tcagcaccgg cgtttacaat ttctatccaa
    23941 ttgccgtcat ctaatgatga ttcggggtga taatttaatt cgctgacagc gattttctgt
    24001 tcatatggcg tgcctgtaaa aacggcagtg aatgtcgttg tataggcatc gatattcact
    24061 ttaaaagtag gattataaat gtcggtaata aatggatttg cttcccaatg atcaaatgtg
    24121 caaccgggaa ggggcacggc agttacctga acttcattgc cattaaaata aacacccgtc
    24181 caaggaaaat cgtaaatatc caaggtgctg atctgaatat gtccgcatcc tgcaggagtt
    24241 gcatttaagg tgacatcgac tttattttca agtgcaaatc catctttaat atggaggcgc
    24301 actttattcg ttctgatact aaaatcgttg cgcaaggcat tgcgataatc ttcgaaagta
    24361 gcgagttgat cttccactgc gccgtagccc catctttcaa gctgtcgcgg taattccgga
    24421 tatatttcgt tataaataga atcttccata cttaatgtac gatctgtgag ataggtgcta
    24481 ttcatcagat cggcatatct atttataaaa tactcacggt atttcggatt ctgaagtaag
    24541 gttgccagcg gctcgatata ttgttgggta taaaataatc ccggaattaa attggcattc
    24601 gcatcagacc atccaccgat tccaagtccc aattccacat caataattgc gtagcgccat
    24661 ttgttatctc ctcccctgtc gcgccaggct ttgatattat tatatggcca gtcgtagttt
    24721 gcaaaccatg tctggcatat gatataatcc gtgaaattgt tgaggtcgag aatagaatcg
    24781 gcgatatcat aaaaatcatc atcggcgggt gtcggataag tgccgaggta gttgcgcatt
    24841 tcaatgaaat ctgtatccga tccgctaagc gtgcgcagta ccaacccata ccaataactg
    24901 agtgtcatca ggtcgaggct gtctttttca atacctttta catgatcaaa atggccttca
    24961 tccaatttgt tccgcaattc atatacaccc caataagcgc cattgata
//
LOCUS       DAOQHY010000027        24065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_919_length_24065_cov_5.045898, whole genome shotgun sequence.
ACCESSION   DAOQHY010000027 DAOQHY010000000
VERSION     DAOQHY010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 24065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24065
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_919_length_24065_cov_5.045898"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3653)
                     /locus_tag="PLL28_03785"
     CDS             complement(<1..3653)
                     /locus_tag="PLL28_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68469.1"
                     /translation="MKYCGLLLIAISLWCTGYSQVTTFSTDPVQFMKDLDDFMKANNN
                     EIGKDSYNRFAKELEATNITAEELDLVITLCNKMKERNMKPTPQFVAVLDATVAFAKS
                     GQLFDRYAEWMEITNAILDKSKKGSYVTYNRFLEFSSDFFSKNALNATPTKSWCYTSF
                     EYQFILNGDVPEVHFPETGLYAFTKGDTMNISATKGVYYPLEKEWRGSDGYVTWARAG
                     LDSSIAYGKLRKYIVNTESNEYIADSATLYFGGLFGKPLIGQLKDHLTTNNEVDKSDY
                     PQFRSYDHELVVKNIMTNVDYKGGFLLKGRQIIGSGSDSIPAVMTFKDPKGKEALVAK
                     SQVFLVEPETSIYSTDAEVMIRVAKTDSIYHHDASIRYDGMLGTVNITRQDEGFSSLM
                     YYSSYHKVDAKVDNLSWTLGDTIIKMKNITGAGNKMSWYESQDLFSAKLFDRVRGVSS
                     YQPLVKIKRYCETYKTRVIPTLALAKELNPSLTVDGVQSLLLQLVEEGFIYYDKEHEL
                     VYVKDKTINYVLASAGKKDYDIINIPSVTKGSNSELNLVNYDLTINGIRQFYLSDSQF
                     VLIYPSNDQITMKKNRDMLFDGTIVSGNIDFKGNNFYFSYDTFSIKMNKVDSMILYVE
                     STAIDDNGNPLYLPVNTNISVSSGRLQIDKTDNKSSKVRYPEYSIFTTYDYSVADYDK
                     PNVFNNVYDKEAFYFKLDPFTLESSDSLDYRKVKFDGTMISAGIFPDFREGLKLQEDR
                     SLGFKTVTPPGGYPTYGKTGKYNGNIYLSNAGFRGSGTIDFMASTSESKDILFFPDST
                     IAKVDDFVVKKTESGIQFPAVTNSGVSTRWLPYEDQMIVSQGTAPFNMFENQATLKGT
                     AIVTSKGLRGDGIMNWGEVKLTSNQFKYLANNAKADTSAVTIGQVTPDLVALKLDNVN
                     SDLDFKERIGHFKANSDTIMTELPYNEYKTDMNVFDWIMDDRVVNFKSTKEYANFYST
                     NDGQDSLMFQAKAGKFDLNTYLLKAEGVPLINIADVQVIPDSGKVQIEKGAKMGTLYY
                     AKIKGDTLNYWHYLDNCVVNISGRLQMSGTGNYTYESPQGKKQNIPIESMNVFKVRDT
                     IMEVTVAHVLAKGYVQDTANFELENKVLFKGPVELYSNEKFLKYRGYAKLVVTDTANI
                     RTSWFHIDTPIDPKNPVFNLNDAVTERDDSVYAGIFRFNDTAALYTNIMGKRASMGDY
                     MVFSARGKGYYNET"
     gene            complement(3764..4597)
                     /gene="prmA"
                     /locus_tag="PLL28_03790"
     CDS             complement(3764..4597)
                     /gene="prmA"
                     /locus_tag="PLL28_03790"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNF68470.1"
                     /translation="MEYLEYNFTTSDEQTAEILIALLADKGFDTFDHNDVQLSAYIRK
                     EDHLESAIAHFLNDLQDRFVFTWSFQAIAPKNWNEEWEKNFEPIRVGKDCLIRAPFHE
                     ADPDVTYDIIIEPRMSFGTGHHETTFLMSQEILGLQMKGKRVADAGTGTGILAILAAK
                     KGAADVFAVDNEEWAWRNAIDNIAINGVIDSVHAELGGLDLMEGKTFDIILANINKHI
                     ILEHIQLFFRCLQPGGLLIVSGMFIRDLQDVEKEAARYFFQLISTQVRNDWVCAVFQN
                     V"
     gene            complement(4605..5369)
                     /gene="tpiA"
                     /locus_tag="PLL28_03795"
     CDS             complement(4605..5369)
                     /gene="tpiA"
                     /locus_tag="PLL28_03795"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNF68471.1"
                     /translation="MSRKKIVAGNWKMYKTLPEAMETIGALTHALAVQSPKENVEVWI
                     APPSVILPMIIHNYHNRGISFGAQNCSEHKEGAYTGEISAAMIKSVEAQFVILGHSER
                     RAYHHENDTLIHKKIRAALSNQLIAVYCCGETLDQRNAGQHFDIVRKQVTEALSGFNA
                     DEMSNIVIAYEPVWAIGTGVTASPEQAQEMHALIRETLRQLFDGKVADQMHILYGGSV
                     KPGNAAELFSQPDIDGGLVGGASLVASDFTGIIYAA"
     gene            complement(5377..6585)
                     /locus_tag="PLL28_03800"
     CDS             complement(5377..6585)
                     /locus_tag="PLL28_03800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmU family protein"
                     /protein_id="HNF68472.1"
                     /translation="MNIILFDDKRWQHLLPLTFTKPVAGIRIGIDTIAEKWNSSVNGT
                     YSFLAEPYLQGKFPVRYGDDNLYIYGGLIPDAQIIAAITSLKTNDVLISGEDILAVRS
                     GERYFSGMSLNEHAAGLNKMHYNGSCICISRPYHIFQHNDAVLRADFARLTAGKKSAE
                     IPAGNTIIGDHIFIEEGAVIHASIINTTTGPVYIGKGAEIMEGSMIRGPFVLGEHSVV
                     KMGAKIYGATTIGPWCKVGGEINNSVFMAYSNKAHDGFVGNSVIGEWCNLGADSNCSN
                     LKNTYAEVKLWDYSLQKFASTGLQFCGLIMGDHSKCGINTMFNTGTVVGVHANIFGDG
                     FPRNFIPSFSWGGAAGFVTYKLKDALTTTKTVMSRRELNLSSEDIKILTTIYELSEKY
                     RKGQLIFLHK"
     gene            complement(6661..6909)
                     /locus_tag="PLL28_03805"
     CDS             complement(6661..6909)
                     /locus_tag="PLL28_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HNF68473.1"
                     /translation="MKKGIHPENYRPVVFKDFSVDFAWLGMSTANTKETIKWEDGNEY
                     PLIKLEISNASHPFFTGKMKFVDTAGRIEKFNKKYAKK"
     gene            7011..7481
                     /locus_tag="PLL28_03810"
     CDS             7011..7481
                     /locus_tag="PLL28_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HNF68474.1"
                     /translation="MHWRTFNGRTIDRPIEEEVRETIERETAAGNKLKVCIGSDSQVY
                     GPIIEFGTVIVFLREGRGGFMYIANETSKQRMSLKERLLHEVSRSIQVAYGLCNLLDE
                     YHVDLEVHADINTNPHFQSNTALQEAMGYILSMGFVFRAKPDSFASSSCANKVV"
     gene            complement(7478..8077)
                     /gene="yihA"
                     /locus_tag="PLL28_03815"
     CDS             complement(7478..8077)
                     /gene="yihA"
                     /locus_tag="PLL28_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNF68475.1"
                     /translation="MLIKTATYEGSFVETNKCPAGGLREFAFIGRSNVGKSSLINMLT
                     GRKELAKTSVKPGKTQTINFFLINEQWHLVDLPGYGYARVSRTLRGTWESMISDYLQK
                     REQLQCVFQLIDGGIPPQKIDLEFTNWLGENGIPFAIIFTKTDKRHKGEGDTPGAYSK
                     EMLKTWENLPPMFITSSTKRIGRDEVLDYLDFILKMPTL"
     gene            8158..8889
                     /gene="ubiE"
                     /locus_tag="PLL28_03820"
     CDS             8158..8889
                     /gene="ubiE"
                     /locus_tag="PLL28_03820"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNF68476.1"
                     /translation="MSEPVTPYHTKASKKEEVRNMFDNIAGRYDLLNRVLSLRIDVYW
                     RNKAVAFLKPFKPAHILDVACGTGDFSIAALKTGAQKVTGVDISEKMLAAGRVKMKER
                     GLDGKITLETGDSEQLQYATATFDAATVAFGVRNFEQLDKGLAEMQRVLKPGAPIVIL
                     EFSKPRVFPVKQLYHFYFSTILPLIGRLVSGDARAYTYLFESVSAFPDGNDFLDHMNK
                     AGFTSCAAKRLTFGICSMYTGLKKD"
     gene            8856..9563
                     /locus_tag="PLL28_03825"
     CDS             8856..9563
                     /locus_tag="PLL28_03825"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNF68477.1"
                     /translation="MFNVHRFKKGLITLLMLVPFLSKAQMNVTEYENRFSIRPYHFGI
                     SLGYNTTDFRIAFADQFIGHDSVMQVLSTPGPGFNLGILTNLRMGKYFDLRFIPTLSF
                     AEKNLNYTYRNESTSTQTVESLYLCMPFDVKFKSAAYKDMKVYVLGGMQYTYDFSSNA
                     EARNAEGLVKIKPNDIAVNAGFGFEFYFPYFIFCPEIKLSNGIVNIHAVDPLLQESYV
                     MDKLLSRTLLFCIHIEG"
     gene            9625..11781
                     /locus_tag="PLL28_03830"
     CDS             9625..11781
                     /locus_tag="PLL28_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNF68478.1"
                     /translation="MKRVFLICLFFIPAILFAQSTISVSDLWVNYRFYPAGIDDLRSM
                     QDGEHYTVNEKGEAIVRYAYADGKKSGDVFNVNQTGGAVTQFQDYHFNADETSILLTT
                     ASERRYRWSSIDENYIYNTKTQQTIKLSDKGGQMYADFSPKQNRIAYVIDNNLYYKDL
                     DNNREYAITTDGKWNEIINGGSDWVYEEEFTQVRSFAWSPDGNKIAYYKFDESEVPEF
                     DMMTYKGQLYPKNYEYKYPKVGEKNSKVVIYIYDIATQKSTVVNIAKPYEYIPRIKWT
                     NDGSALCVFTMNRLQNDLELFLADPATGNTRSMFHETAKYYLEINDDLTFLKDNKSFI
                     WRSEQDGWNHLYRYDMNGKLINQITSGKFEVTQVLGINEEKGVVYYMSTEESPYERHL
                     YATQLDGKKTKKITSRKGNNSVNFSTGFKYFINTNSTFMQPDYITLCDGAGKEIRVLE
                     DNAALQKDVDQLDIAAPQFMQIPGADGNGLNAYMIKPKNFDASKKYPVLMTVYGGPGS
                     QEVIDGFDGFNMMWHQMLAQNGYIIVCVDNRGTGARGRDFRQVTYGQLGKFETDDQIA
                     AAQWLGKQTYIDASRIGIWGWSYGGYMAGLCITRGADVFKTAISVAPVTNWKYYDNIY
                     TERYMGTLETNPTGFDANAPMAYADMLKGNYLLVHGTGDDNVHFQNSVEWINALVRNN
                     KQFDLMVYPDRNHGIYGGKTRLHLYTLMTDYILENL"
     gene            complement(11786..12571)
                     /gene="folP"
                     /locus_tag="PLL28_03835"
     CDS             complement(11786..12571)
                     /gene="folP"
                     /locus_tag="PLL28_03835"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNF68479.1"
                     /translation="MGILNVTPDSFYDGGRFVEGLSAVEQAERMVKAGVDIIDIGGMS
                     TRPGADIISPREEMDRVLPVIAEIRVEFPEMLISIDTVYGRTAREALKAGADIINDVS
                     AGTMDSDIFQAAIDHQAPYILMHMQGNPQTMQLEPYYEDVVEEVLTFLSEKTTSLKHH
                     GLQQVILDPGFGFGKTLTHNYQLLASFEKFLALGCPVLAGVSRKSMICKLLHISPDHA
                     LNGSTALHALLLQKGAHILRVHDVKEAAQTRAIFMAMQNPLGL"
     gene            12658..13230
                     /locus_tag="PLL28_03840"
     CDS             12658..13230
                     /locus_tag="PLL28_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HNF68480.1"
                     /translation="MSTKLMQSDVSKTNLQYDDVVEGCRNIYLKKIKDYGTSWRILRP
                     TSLTDQIFIKAQRIRTLEQTGVSRVGEGIHDEFRGIINYAIIALIQLEMGENGPVNLS
                     EEKALELYNEKIGKVKKLMLDKNHDYGEAWRDMRISSFTDLILMKLLRVKQIEENDGK
                     TLISEGVDSHYSDMINYSVFALIKLSEDEK"
     gene            13220..14899
                     /locus_tag="PLL28_03845"
     CDS             13220..14899
                     /locus_tag="PLL28_03845"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HNF68481.1"
                     /translation="MKNKLLTICRILTGGLFIFSGLVKANDPVGFGIKLEEYYDVFAG
                     RWSWTAWLFHSDLMLNTVNLQAAFLTTLEVALGIALLMGLWKNLVSWLLLLLILFFTW
                     LTGYSAITGSVTDCGCFGDFIKLTPLQSFYKDLVLLALILVIFFLRKGLKPIKPLGIS
                     IGLFLTVTVFAIWVNVHVLQHDVFLDWRPYAVGDNITENMKLPENAQPDITEMIYAYT
                     NKKSGEQVTLKYLNTELKEKKNLDELLSYSNNKDTWKLDTSYTKIKVKGDRAKIIDFA
                     ISDEHDEFITDQILGNPDFALMVVSAGFEHTNKEGWDRISAVQKDAEKEGLFTFALVG
                     EGRTEIEKFRHNQQMAFPFYTADYKVCLTIARVNPNVVLMKEGTIIAKWAWRDLPSFE
                     EIKKTYFPERKPTELMVISNEMFAPGIQVSAKLRDAEEPYNEFFLQDTVGNDLTTALI
                     NDTTNAVMFIINELSSDKLTMENWKSILAVMQMSNTAQKPFFVASSGSPEVLEMMKKA
                     SGLEFGYYVSDKDVLAKMMETNTGLIVIKNGKVTAKYADGKLPSDSNFLTQ"
     gene            14909..15985
                     /locus_tag="PLL28_03850"
     CDS             14909..15985
                     /locus_tag="PLL28_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF68482.1"
                     /translation="MISYIIKRLGYGVLVLIGVVIVVFFLFQLTRIHPERQIAGEKAD
                     KETIENIKKELGLDLPVSQQLLLYLNDISPLSFHQVADSSSRYFLDGGKYDYFRLFTI
                     NHTATVLKKPYLSRSYVSKRSVNEILSQRIPNTLVLALAAMLLATVVGIALGVIAAVK
                     QGTAIDRLFTAGSVLGISFPSFFAGIILQLIFAYLLASITGFNMTGNLYETDPYTGER
                     YLALQNLILPAIALGIRPIAVITQLTRSAMIDVLSNDYIRTARAKGLSERKIVFRHAL
                     KNALNPVVTSISGWLAGLLTGAFFIEAIFGYNGIGYETVNALKTFDYPVAMGAILYTA
                     VIFVVINIIVDILYSVIDPRIRIA"
     gene            15982..16494
                     /locus_tag="PLL28_03855"
     CDS             15982..16494
                     /locus_tag="PLL28_03855"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNF68483.1"
                     /translation="MRVYLIGFMGCGKTTFGKKLARKMNVDFIDLDKQIEAHEGKSVS
                     EVFAEKGEAYFRELESSLLQQTISLNHAVISCGGGTPCFHDNMHWMREHGITVYLKVT
                     PEFLFSRLHTRREKRPLIAKLNDEELQAYITTKLEEREVHYQQAQQVIDPVHAKPGFV
                     ADLLNARKGN"
     gene            complement(16501..17016)
                     /locus_tag="PLL28_03860"
     CDS             complement(16501..17016)
                     /locus_tag="PLL28_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNF68484.1"
                     /translation="MEQTLTQIISPEKVLQKIKRIAFEIAEDNYDEPEIVLAGICKET
                     EGYAFAELIYQELKKISSAHIILTHVELDKHNPGDSVVELNLTEEQIDNKVIILADDV
                     SNTGRTLLYAIKPLLSYSPKKIRSVVLVERKHKLFPVTPDYVGLSLSTTIHEHIKVVL
                     SDKQHAGVYLM"
     gene            complement(17020..19464)
                     /locus_tag="PLL28_03865"
     CDS             complement(17020..19464)
                     /locus_tag="PLL28_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNF68485.1"
                     /translation="MPKTLLIVLLAAICFSADAQQSSPGSCIGLSAYHAMNDAAPGEQ
                     IPVYIKGDVPALAALIKQYGGTFKFAAGDIISATLDRAAIRAINESGLAVRIEANANK
                     GNLLNDVMVLHNNVDSAFYGYWPLEQAYDGTGVVVGIIDAPFDLDHGDFTDADGNSRI
                     KYVWDQNITTGAPPTPYGYGVECDSAMIADGTCPSVDDDPLHYSHGSGVTGVAASSGM
                     ASNHYRGVAPNADLILVSMDFNNDYETNATDAIAYIYAKAAEMGKPCVINTSFGSYAG
                     SHDGSDLMSQTIDALITASPGRALVAAAGNAGNNAFHLGYDVTATEQFTWFKKLSYMN
                     LVYFQLWADTTDFNQVDFRLSVDEPAGFTSLGSTPYFNMLSDFDFTGDIIDSVTYSIP
                     GGGNITIYAQLDNDKYFLEFVATPSVPGNYWRLSTVGSGHFDIWSAESTTGFSNYVTT
                     GLPSAVVLPEIVNYRLPDTDQTIVSSFQCSDHVITVGSYVNRDSMTNFYLENPPFFDV
                     VGALFYSSSHGPTRDGRIKPDICAPGARVLSTASDTLTEWLALLDAATYVSADAQHYL
                     YNGTSFSSPAVAGIAALYLQKNPNANAAEIKDAILSQARKDDFTGAALPNNLWGYGKA
                     DAFRTLTGDWGCAADDYTEPPVDLQVLATTPTKAMLDWDIIPNAAGYQIWYKSSGGSW
                     NKLKSPSHTKTISGLTPNTTYNAKVRSYCTGFGFSDFSTTITFTTPPLREDISSEMLI
                     NVYPNPASNILHVDGVDAGSSIVIVNMLGQVIYNQTTTNAETNQIDIHDWAEGMYRLL
                     INEHDNYTLHTFIVAR"
     gene            19531..20844
                     /locus_tag="PLL28_03870"
     CDS             19531..20844
                     /locus_tag="PLL28_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNF68486.1"
                     /translation="MEIVLQVLFAVVFGVSFYLAGRKFVAIRRNILLGRKESLTDQPA
                     RRWKHMVLFALGQKKMFRKPLPAVLHLFIYVSFVIVNLELIEIAIDGVTGNHRTFAPY
                     LGGFYTFVISLIETLSALALVATLVFLWRRNVLKVKRFTQPELKGWPFKDANFILGME
                     LILVTAILTMNGADYRLQQLGAAHYVDTGTLLVGQFTSQLFMHMGTASLEHVERIAWW
                     VHILGILFFLNYIPYSKHLHIILAFPNAYYTRFAVAGKMENMPVIANEVKSMLDPSAA
                     ITEIPDPPKTFGAKDVTDLTWKHLLDAYACTECGRCTAACPANITGKQLSPRKIMMDT
                     RDRLEEVGRNFDKHGAITDDGKSLFGDYITAEEIRACTTCNACVEECPVGIQPLSIIL
                     ELRRYAVMEQSNAPNEWNMMFNNMENNQAPWQFNPGDRVNWKNEI"
     gene            20881..21681
                     /locus_tag="PLL28_03875"
     CDS             20881..21681
                     /locus_tag="PLL28_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNF68487.1"
                     /translation="MQENLTNVPIMAELFANGESPEVLFWVGCAGSFDDRAKKITGAF
                     VRILNSIGVRFAVLGTEESCTGDPARRAGNEFLFQMLAQNNIAVMNGYGIKKIVTTCP
                     HCFNILKNEYPALGGEYEVLHHTAFLQQMIDEGRLKVEGGAFAGKRITYHDSCYLGRA
                     NDIYEAPRNVLIQLDAQLAEMKSCKSRGLCCGAGGAQMWKEAEHGNKEINIERTEQAL
                     EVNPDIIASACPFCNTMMTDGVKMKEKEEGVKVFDVAELVAMANGLFA"
     gene            21678..22184
                     /locus_tag="PLL28_03880"
     CDS             21678..22184
                     /locus_tag="PLL28_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68488.1"
                     /translation="MIATENNSIEKLIADFDPASKVWVYQSSRPFTSAEENEISQALQ
                     SFTKQWAAHNIQLKATGFIVESRVIVLMVDETHTDASGCSIDTSVHFIQGLENEYGVE
                     LFDRMIVHYRDVEGWHTTTLSTLPDLISQHLISKSTIVINPLVATKEEYQHSFVTRIE
                     DCWMMQFV"
     gene            22228..23514
                     /locus_tag="PLL28_03885"
     CDS             22228..23514
                     /locus_tag="PLL28_03885"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HNF68489.1"
                     /translation="MKTLLTLVFCTFLSLASFAQATDSTKTDSINSAYLNLYLDGGYG
                     YQDYIKTEIPFVNYVRDRFDGDVHLLITAQGTGSGGADYTLFFLGQKTFEGKNDTLHY
                     IANTNATSEEIRAGLVQVIKMGLLPFIAKMNAAFPVQITFTGSTNSEERQENDKWKGW
                     VYSISSYANASLTSNYESYSISGNVSADKVTEEWKLGFSYGANYNTDVFDFGDGDIYE
                     SFNNSTYGNATIVNSLNDHWSLGGEGFYSTSTYSNYKTNIAIAPGIEYDVFPYDESTT
                     KLLTFFYKVGPEYHHYIDSTIYNKTEELLMRQRLDVSLSLTQKWGSITVGINGSNYFH
                     DLTKNGAGCFTSINWRIVEGLSLNGFFTLDFINDQLNIPKGDLTDEEILLQITERQTN
                     YSFYSFFGLSYTFGSIYNNVVNPRFSGGSYSFYFSN"
     gene            23603..24064
                     /locus_tag="PLL28_03890"
     CDS             23603..24064
                     /locus_tag="PLL28_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68490.1"
                     /translation="MHDNYYVFDTIMGAKYPFWYMVILFIFQMGALVKDNWVLTVAFL
                     PMFIIFAMYEGVEVNIAKMQYRDYTHLFQWWWGRFKPIEDPDYLLLSRTSDTRNGGIR
                     YDVHLITKGSRDLLLFRTKNLEDHENVVKVLAQRTGWKAYRNTIDGLEGFE"
BASE COUNT         6877 a         4890 c         5208 g         7090 t
ORIGIN      
        1 gtttcattat aataaccttt tccgcgagca gagaacacca tgtaatcacc catgctggcg
       61 cgtttaccca taatgttggt atacaatgct gcagtatcat taaatcggaa aattccggca
      121 tatactgagt catcgcgctc ggttacggca tcatttaaat tgaataccgg atttttcgga
      181 tcaattggag tgtcgatatg gaaccagctt gtgcgaatat ttgctgtgtc ggttaccaca
      241 agttttgcat aaccacgata cttaagaaat ttttcattag aatataattc taccggtccc
      301 ttgaacagta ctttgttttc caactcaaag tttgctgtgt cttgcacata accttttgcc
      361 aaaacgtgag ctacagtaac ttccatgatg gtatcgcgaa ccttaaatac attcatgctc
      421 tcgataggaa tgttttgctt tttaccttgt ggtgattcgt aagtgtaatt accggttcca
      481 ctcatttgta aacgtccgct gatatttacc acgcaattat caagatagtg ccagtaattc
      541 aaagtatcgc ccttgatctt ggcataatac agcgttccca tcttcgcacc tttttcaatc
      601 tgcacttttc ctgaatccgg aatcacctgt acatcggcaa tattgattaa tggaacgcct
      661 tcagctttta acaagtaagt attaagatcg aacttacctg ccttggcctg aaacattaag
      721 ctgtcctgac catcgttggt cgaatagaag ttggcatatt cttttgtcga tttgaaattt
      781 actacgcggt cgtccattat ccaatcaaat acattcatat ctgttttgta ttcgttgtat
      841 ggtaattcag tcataatggt atctgaattc gccttaaagt gtccaatgcg ctctttaaaa
      901 tctaaatctg aatttacatt atcaagcttc aacgctacca gatccggcgt tacctgtccg
      961 atggtaactg ctgacgtatc ggctttggca ttattagcga gatatttaaa ttgattggaa
     1021 gtgagtttta cttctcccca gttcatgatg ccatcgccac gcaagccttt tgatgttaca
     1081 attgctgttc ccttcaatgt ggcttgattc tcaaacatat taaatggtgc tgttccctgt
     1141 gaaacaatca tctgatcttc atatggtaac cacctggtgc tcacaccgct atttgttaca
     1201 gcaggaaatt gtattccact ttcagttttc ttgacaacaa aatcatcaac tttggcaatg
     1261 gttgaatccg ggaaaaacag gatgtcttta gattcagaag tagaagccat aaaatcaatc
     1321 gttcccgatc ctctgaaacc ggcgttactg agataaatat tgccattgta tttaccggtt
     1381 tttccataag taggatatcc acccggagga gtcaccgttt taaaacctaa tgacctgtcc
     1441 tcttgtagct ttaatccctc tctgaaatcg gggaatatac ctgcagagat catcgttcca
     1501 tcaaacttga cctttcgata atccagtgaa tcggaactct ctaaagtaaa tgggtctaat
     1561 ttgaaataga atgcttcttt gtcatacaca ttgttaaata cgtttggctt gtcgtaatct
     1621 gctacggaat aatcataagt ggtgaatatg gaatattcag gatagcgcac cttgctcgat
     1681 ttgttatcgg ttttatcaat ttgcagtcga cctgacgaaa cactgatatt ggtatttacc
     1741 ggcagataaa gtggattgcc attatcatcg atcgcagtgc tttcaacata aaggatcatg
     1801 gagtccacct tgttcatttt gatgctgaag gtgtcgtagc tgaaatagaa attgtttcct
     1861 ttgaaatcta tgttaccgga aacaatggtg ccatcaaaca gcatgtcgcg attttttttc
     1921 atggtgatct gatcattgct gggataaatt aacacgaact gagaatcgct gagataaaat
     1981 tgacggatac cattgatggt taaatcgtaa ttcaccaggt tgagctctga gttagaacct
     2041 ttggtaactg atggaatatt gataatgtcg taatcttttt ttcctgcaga agctaataca
     2101 taattgatgg ttttatcctt tacatataca agttcatgtt ccttgtcgta gtagataaat
     2161 ccttcttcca ctaattgcaa caataaactt tgaacgccat caacagtaag gctagggttg
     2221 agttctttcg ccagtgccaa tgtgggaatc acacgtgttt tataggtctc gcaatagcgt
     2281 tttattttca ccagcggttg atatgaggat acacctcgca cgcgatcaaa taatttcgca
     2341 ctgaataaat cctgactttc ataccagctc attttatttc ctgcgccggt gatgtttttc
     2401 attttgataa tggtatcgcc gagtgtccaa ctgagattat caacctttgc atcaacctta
     2461 tggtaggaac tatagtacat aaggctagaa aatccttcat cctgccttgt gatgtttaca
     2521 gtccccaaca tgccatcata gcgaatagag gcatcatggt gataaattga atcggttttc
     2581 gcaacacgta tcatcacttc tgcatctgtt gaataaatcg aagtttccgg ctcaaccaga
     2641 aatacctgcg atttagctac caacgcttcc ttccctttag gatctttgaa tgtcatcaca
     2701 gcaggaatgc tatcgcttcc ggatccaatg atttggcgac ccttgagtaa aaatccacct
     2761 ttgtaatcaa catttgtcat gatgtttttt acaaccagtt catgatcgta tgacctgaat
     2821 tgcggataat cgcttttgtc tacttcgtta ttcgttgtga ggtgatcttt gagttgacca
     2881 atcaatggct ttccgaaaag tccgccgaaa tataaggttg cagaatcggc gatatattca
     2941 ttagattctg tattcacaat atatttgcgc aattttccat aagcgataga gctatcgagt
     3001 ccggcgcgtg cccatgtaac ataaccatcg ctgccacgcc actctttttc gagcggataa
     3061 tacacacctt tggtggcact gatattcatg gtatcacctt tggtaaatgc atataatcct
     3121 gtttcaggaa aatgcacttc cggtacatcg ccattcaaaa tgaattgata ttcaaaagag
     3181 gtatagcacc atgattttgt cggcgttgcg ttcagtgcat ttttagagaa aaaatcagac
     3241 gagaattcca ggaagcgatt gtaggtaaca taacttcctt ttttcgattt atcgagaatg
     3301 gcattggtga tctccatcca ttcggcatag cgatcgaaca attgtccgga ttttgcaaag
     3361 gctacagtcg cgtctaatac ggcaacaaat tgcggagtgg gcttcatatt gcgctccttc
     3421 attttattgc acaaggtgat caccagatcg agttcttcgg cagtgatatt ggtggcttcc
     3481 aactctttgg caaagcgatt ataactgtct tttccgatct cattgttgtt tgccttcatg
     3541 aaatcgtcca gatctttcat gaattgcaca ggatcagtag aaaaggtggt gacctgcgaa
     3601 tagccggtac accaaagact gattgcgata aggagaagtc cacaatattt catgccgtta
     3661 atttaaagta cacagggaat acctgcggta ttcaaaacgt agataacccc tttacagtat
     3721 gggattaaca caaattaaat gcctgaatta tgggctaaag tgcctataca ttctgaaaaa
     3781 cggcgcaaac ccagtcgttg cgcacttgtg tggaaattaa ttgaaagaaa tacctggcag
     3841 cctctttttc cacatcttgt aagtcgcgga taaacatgcc gctcactatc agcagtcctc
     3901 ccggttgcaa acagcggaaa aacagttgga tatgctccaa aatgatgtgt ttattgatgt
     3961 ttgccaatat tatatcaaaa gtcttgccct ccatcaggtc taatccgccc aattcagcat
     4021 gaacactgtc tattacaccg ttgatcgcaa tattatcaat ggcattccgc caggcccatt
     4081 cctcattgtc aacggcaaat acatcggcgg ctcctttttt ggcagctaaa atggctaata
     4141 ttcctgttcc ggtgcccgca tccgcaacgc gcttaccttt catttgaagt cccagtatct
     4201 cctgcgacat taaaaatgtt gtttcatggt gcccggtgcc gaagctcatg cgtggctcga
     4261 tgatgatatc gtaggtgaca tccggatcag cttcgtggaa tggtgctctg atgagacaat
     4321 cctttccgac acgaataggc tcgaaatttt tttcccattc ctcattccag ttttttggtg
     4381 ctattgcctg aaatgaccag gtaaatacaa acctgtcctg aagatcattg agaaaatgtg
     4441 caatcgcact ttccagatgg tcttccttgc ggatataagc acttaattga acatcattgt
     4501 gatcgaaagt atcaaatccc ttgtcggcaa gtaatgctat taatatttca gcagtttgct
     4561 cgtccgacgt ggtgaaattg tattcgaggt attccatgat gcaatcaggc tgcgtaaata
     4621 atacctgtaa aatctgatgc aacaagactc gcaccaccta caagaccgcc gtctatatcg
     4681 ggctgtgaaa ataattcagc tgcattaccg ggttttacac tgccaccata aagtatatgc
     4741 atctgatcag ctacttttcc gtcaaataat tgtcgtaatg tttcgcgaat aagtgcatgc
     4801 atttcctggg cttgttccgg agaagcggta acgcctgtac caattgccca aaccggttca
     4861 tatgcgataa cgatatttga catttcatcg gcattaaatc cgctcaaggc ctcggtaact
     4921 tgtttgcgta cgatgtcaaa atgctgaccg gcatttcgtt ggtccaatgt ttcaccacaa
     4981 caatacacgg cgatcaattg attggataat gcagcgcgaa tttttttatg aatgagtgtg
     5041 tcattttcat gatgataggc tctgcgttcg ctgtgaccta aaatcacaaa ttgtgcttcc
     5101 acacttttaa tcatcgcagc acttatttct ccggtatatg cgccttcctt atgttcactg
     5161 caattttgtg caccaaaact gattccacga ttgtgataat tgtgaataat cataggtaat
     5221 atcactgaag gaggagctat ccaaacttct acattttctt ttggcgattg cactgctaaa
     5281 gcatgtgtca gtgcaccaat ggtttccatc gcttcaggaa gtgttttgta catcttccaa
     5341 ttccctgcaa caattttctt tctgctcata gttgtattat ttatgcagga atatcaattg
     5401 acctttcctg tatttttcac ttaattcata aatggtggtg aggattttaa tgtcctctga
     5461 tgataaattt aattcgcgtc gtgacatcac agtttttgtt gtggttagtg catccttcag
     5521 cttataggtg acgaatcctg ctgcaccgcc ccagctgaaa gaaggaatga aattgcgcgg
     5581 aaagccatcg ccgaaaatat tggcatgcac acctacaacg gttccggtat tgaacatggt
     5641 gttgatgccg cacttgctgt gatcgcccat gattaaaccg caaaattgta atcctgttga
     5701 ggcaaatttt tgaagcgaat aatcccataa tttcacttcg gcataggtat ttttcaggtt
     5761 ggaacaattg ctgtcggcac ctagattaca ccattcgcca ataacactgt ttcctacaaa
     5821 gccgtcatgc gctttattgg aatatgccat gaatacacta ttgttgatct cgccaccaac
     5881 cttacaccat ggacctatgg ttgtggcgcc ataaattttc gcacccattt ttactacgct
     5941 atgttcacct aatacgaatg gtcctctgat cattgaacct tccattattt cagcgccttt
     6001 tccaatgtaa acaggtcctg ttgtcgtgtt tatgattgat gcatgaatga cggccccttc
     6061 ctcaatgaaa atatgatcgc caataatggt attgcctgcc ggaatttctg cactcttttt
     6121 tcctgccgtt aagcgagcaa aatcagccct taatactgcg tcgttatgtt gaaatatgtg
     6181 atatggtctt gaaatacaaa tgcaacttcc gttgtaatgc attttattta atcctgcagc
     6241 gtgttcattc agcgacatac ccgaaaaata gcgctcaccg cttcgcaccg ctaatatgtc
     6301 ttcacctgaa attaatacgt cgttggtttt taatgatgtg atggctgcaa taatttgtgc
     6361 atcaggaatc agaccaccat aaatgtacag attgtcatcg ccataacgaa ccggaaattt
     6421 gccttgcaga taaggttccg ctaaaaatga atatgtacca tttacggagg aattccattt
     6481 ttcggcaata gtatctatgc cgatgcgaat accggctaca ggttttgtaa aagtgagtgg
     6541 taacaaatgc tgccaacgct tatcatcgaa gagtataatg ttcatgatgc gacaaattta
     6601 aatgaaaaac cccttctgca cacaaggtac ggaaggggtt taataggttt tagccgatta
     6661 ttatttcttg gcgtattttt tattgaattt ctcgatacga cctgctgtat caacaaactt
     6721 catcttaccg gtgaagaatg gatggctggc gttgctgatc tctaatttga tcagggggta
     6781 ttcattaccg tcttcccatt tgatggtttc tttggtgtta gcagttgaca tacctaacca
     6841 ggcaaagtct acgctgaagt ctttaaatac taccggacga taattttcgg gatgaatgcc
     6901 ttttttcata actactgtta attgaaggtg caaaggtact gctttttttt cattcccgga
     6961 aattatttcc cgcaatttat gcgtatactt gtagtatgac gggcacaacc atgcactgga
     7021 gaacttttaa cggcaggacc atcgaccggc cgatagagga ggaggtgcgt gaaactattg
     7081 agcgggaaac ggctgcaggc aataaactga aggtctgcat cggttccgac tcgcaggttt
     7141 atgggcctat catagagttc ggcaccgtta tcgtgtttct gcgcgaaggc agaggcgggt
     7201 ttatgtatat cgccaatgaa acctccaaac aacgtatgag cctgaaagag agactgttac
     7261 atgaggtttc aaggtcaatt caggtggctt acggactatg caatctactc gacgaatacc
     7321 acgttgatct ggaggtgcat gccgacatca atacgaatcc gcattttcaa tccaataccg
     7381 ccctccagga agctatgggt tatatcctca gcatgggctt tgttttcagg gcgaaacctg
     7441 attcttttgc ctccagcagt tgtgccaata aggtggtcta aagggttggc atcttcagta
     7501 taaagtccag ataatcaagc acttcgtccc ggccaatgcg ctttgttgaa gaggttatga
     7561 acatgggagg caggttttcc caggttttaa gcatttcctt gctatacgca ccgggggtat
     7621 cgccttcgcc tttgtggcgt ttgtctgtct ttgtgaaaat gattgcaaag ggtatgccat
     7681 tttccccaag ccagtttgtg aattctaagt ctatcttctg aggtgggatg ccgccatcaa
     7741 tgagctgaaa cacgcattgc agctgctccc gcttctgcaa ataatcgctg atcatgctct
     7801 cccaagtacc cctcaaagtc ctcgaaacgc gagcatagcc gtatccggga agatcaacca
     7861 gatgccattg ctcattgatg agaaaaaaat tgatggtctg ggtttttccg ggcttcacac
     7921 tcgttttggc gagctctttg cgaccggtaa gcatattgat gagcgatgat ttcccaacgt
     7981 tagagcggcc gataaaagcg aattcccgca gtccgccggc aggacattta ttcgtttcaa
     8041 caaaacttcc ttcgtatgta gctgttttaa taagcacgcg gcaaaggtac ggtttcaaac
     8101 acgaattgac atcctgatac ctgcatgccg atattttact actacctttg cacccgcatg
     8161 tcggaaccgg taactccata tcataccaaa gcctccaaaa aggaggaagt gcgcaatatg
     8221 ttcgacaata ttgccggtcg ctatgacttg ttgaacaggg tattgtcgct gcgtatcgac
     8281 gtttattgga ggaataaggc tgttgcattt ctcaaaccct ttaaacctgc tcatattctt
     8341 gatgtagcat gtggaacagg tgatttttct atcgcggctt tgaaaaccgg tgcacagaag
     8401 gttacgggcg ttgatatctc agaaaaaatg ctcgccgccg gaagggtgaa aatgaaagag
     8461 cgtggacttg atggcaagat tactcttgaa acgggagatt ctgagcaatt gcaatatgct
     8521 acagccacct tcgatgccgc aaccgttgcg tttggtgttc gaaattttga gcaactggat
     8581 aagggtcttg cagagatgca gcgcgtgctt aaacccggag ctccaattgt gatcctggaa
     8641 ttctccaaac cgcgtgtgtt tcccgttaaa caactctatc acttctattt cagcacaata
     8701 cttccgctaa taggtcgttt agttagcgga gacgcaaggg catacaccta tttgttcgaa
     8761 tcagtgtcgg cattccccga tgggaacgat tttctcgatc acatgaataa agcaggattt
     8821 acctcatgcg ctgcgaaaag gctaacgttc ggaatatgtt caatgtacac cggtttaaaa
     8881 aaggattgat cacattgctg atgctcgtgc cattcctgtc gaaagcacag atgaatgtca
     8941 ctgaatatga gaatcgcttc tccatcaggc cctatcattt tggtatttca ctgggttata
     9001 atacaactga cttcagaata gcttttgcag accagtttat tggtcatgat tcagtaatgc
     9061 aggtgctttc cactcccggt cccggcttta acctcggaat tttaacaaat ctgcgcatgg
     9121 gtaaatattt cgaccttcga tttattccga ccctgagctt cgctgaaaaa aatcttaact
     9181 acacttatcg caatgagtct acctctacgc aaacggtgga atcgctatat ctgtgcatgc
     9241 cttttgatgt gaaatttaaa agtgctgcat ataaggatat gaaagtgtat gtacttggag
     9301 gcatgcagta tacctacgat ttcagctcca atgccgaagc ccgaaacgcg gaaggacttg
     9361 ttaaaatcaa acccaatgat attgcggtca atgccggatt tggatttgag ttttacttcc
     9421 catatttcat attctgcccc gaaataaaac tcagtaatgg catcgtaaac atccatgccg
     9481 tagatccgct gctgcaggaa agctatgtga tggataaatt acttagcaga acgctcttgt
     9541 tctgcataca tatcgaaggc tgatggttgc tgcatagtat ctgaatgaca attctttacc
     9601 tttacagata aaataaacat cattatgaag agagtattcc tcatctgtct gttttttatt
     9661 ccggcaatct tattcgccca atctactatt tcggtatccg acctttgggt gaattatcga
     9721 ttttatccgg ccggtattga cgacctccgc agcatgcaag acggcgaaca ttataccgtg
     9781 aatgaaaaag gtgaagctat tgtgcgttat gcttatgctg atgggaagaa gagtggtgac
     9841 gtctttaacg taaaccagac aggaggtgct gttactcagt ttcaggatta tcattttaat
     9901 gcagatgaaa cctccatttt gctcactaca gcatcggagc gcagatatcg ctggtcgtcg
     9961 attgatgaga attatattta taataccaaa acacagcaaa cgataaaatt atctgacaag
    10021 ggtggacaga tgtatgccga tttttcgcca aagcaaaatc gcatagcgta tgtaattgat
    10081 aataatttat attataagga tctggataat aatcgcgaat acgccattac cactgatggc
    10141 aagtggaatg aaatcatcaa cggtggcagc gattgggtgt acgaagagga attcacgcag
    10201 gtgcgttcat ttgcatggag ccctgatggc aataaaatag cctattacaa attcgatgag
    10261 agcgaagtgc cggaatttga tatgatgacg tataaggggc aattgtatcc caaaaattat
    10321 gaatataaat atccgaaagt aggcgagaag aacagcaagg tggtgattta tatttatgat
    10381 atcgctacac agaaatctac tgtcgtaaat attgcaaaac cttatgaata cataccgcgc
    10441 atcaaatgga caaacgatgg ctctgctttg tgtgtattta cgatgaacag gttgcaaaat
    10501 gaccttgaat tatttcttgc tgatccggct acaggtaaca ccagaagcat gtttcatgaa
    10561 acagcaaaat attatttaga gataaatgac gaccttacct tcttgaaaga caataaatct
    10621 ttcatctggc gcagtgaaca ggatggttgg aatcatttat atcgctatga tatgaatggt
    10681 aagctgatta atcagatcac ttcaggaaaa tttgaagtaa cgcaagtgct gggtataaat
    10741 gaagaaaaag gtgtggtata ctatatgagt acagaagaat caccttatga acgtcatttg
    10801 tatgcaacgc agttagatgg taaaaagacg aaaaaaatca cctctcgcaa aggcaacaac
    10861 agcgtgaatt tcagtacagg gtttaaatac tttatcaata ccaattcaac ctttatgcag
    10921 cctgattata tcacgttgtg tgatggtgca ggcaaagaaa tccgcgtatt ggaagataat
    10981 gctgccttac aaaaagatgt tgatcaactt gatatagcgg caccgcaatt catgcaaatt
    11041 ccgggcgctg atggcaacgg attgaatgcc tatatgatta aacctaaaaa tttcgacgcg
    11101 tctaaaaaat atccggtact gatgacagtg tatggcggtc ccggttcgca ggaagtgatt
    11161 gatggttttg atggatttaa tatgatgtgg catcaaatgc ttgcacaaaa tggatatatt
    11221 attgtgtgtg tagacaacag aggaacaggt gcaagaggtc gcgatttcag gcaagttact
    11281 tacggtcagt taggaaaatt cgaaaccgac gatcagatag ccgcagcgca atggttaggt
    11341 aagcaaacat acattgatgc ttcacgcatt ggtatttggg gatggagcta tggcggttat
    11401 atggcaggat tatgtattac aagaggtgct gatgtgttta aaacggcaat ctcagttgca
    11461 ccggttacga actggaaata ctacgacaat atctacaccg aacgctatat gggaaccttg
    11521 gaaacaaatc cgaccggatt cgatgcaaac gcgccaatgg cttatgcaga tatgttgaaa
    11581 ggaaattatt tattggtgca cggaaccggt gatgacaacg tgcatttcca aaatagtgtg
    11641 gagtggatta atgcactcgt gcgcaacaat aagcaattcg acctgatggt gtatcctgat
    11701 cgaaatcacg gtatctacgg tggcaaaaca aggttgcact tgtatacctt gatgacggat
    11761 tatatattgg aaaacttgta aggaattaca accccaacgg gttttgcatg gccatgaaaa
    11821 tggctcttgt ttgagcagct tcttttacgt cgtgtacccg aagaatatgt gctccttttt
    11881 gcaataataa agcatgcaat gccgtgctgc catttaatgc gtgatcagga gaaatgtgta
    11941 ataatttaca gatcatactt tttcgggaaa cgcctgcaag aacagggcaa ccaagtgcta
    12001 ggaatttttc aaaagatgcc agtaattggt aattatgggt cagcgttttc ccaaatccaa
    12061 atcctggatc caatatcacc tgttgcaatc cgtgatgttt taatgaggtg gttttttctg
    12121 ataaaaaagt caaaacttct tccacaacat cctcatagta tggttctaat tgcatggttt
    12181 gtggatttcc ctgcatgtgc atgagaatat acggcgcctg atgatcaatc gcggcctgga
    12241 aaatatcact gtccatggta cctgccgaga catcattgat aatatcggca ccggctttta
    12301 gtgcttctcg ggctgttctg ccgtaaacag tatcgatgga tatgagcatt tcaggaaatt
    12361 ctacccttat ttcggcaata accgggagta ctctgtccat ttcctccctg ggagaaatga
    12421 tatcagcccc cggccttgtg ctcataccgc caatatcaat gatatcaaca ccggccttta
    12481 ccatacgctc agcttgttct acggcactta aaccttccac aaacctgccg ccgtcataaa
    12541 aggagtcggg cgtgacattc agaataccca tgattgcggg tttcgagaag tcaagcgtgt
    12601 gcctcgaggt gtgtaacgct aggttagggt agaaatcggt atttttgtcc attggtaatg
    12661 agtacgaaat taatgcaatc tgacgtgtct aaaacgaatt tgcaatatga tgacgttgtt
    12721 gagggctgcc gcaacatcta tctgaagaag atcaaggatt atggaacctc atggcgtatc
    12781 ctgcgtccga cctcgctgac agatcagatt ttcatcaaag cacagcgcat ccgaacattg
    12841 gaacaaacgg gtgtgagcag agtgggagag ggaatccacg atgagttcag aggtattatc
    12901 aattatgcca tcattgcgct tattcaactt gaaatggggg agaacggccc tgtgaacctt
    12961 agcgaagaaa aagcgttgga actatacaat gaaaaaatcg gtaaagtaaa aaaactgatg
    13021 ctcgataaaa atcacgatta tggtgaagcc tggcgtgata tgcgcatcag ctcatttacc
    13081 gacctgatct tgatgaaatt attaagggta aaacaaattg aagaaaacga cggcaaaaca
    13141 ctgatatccg aaggtgttga ttcgcactat agcgatatga tcaattattc ggtgtttgcc
    13201 ttgatcaaat tgagtgaaga tgaaaaataa actgttgacc atctgccgca ttttaaccgg
    13261 cggactattt attttttcgg gtttagtaaa ggccaacgac cccgtaggat tcggcatcaa
    13321 gctggaagaa tattacgatg tatttgcagg acgctggtcg tggacagcat ggctgtttca
    13381 tagcgacctg atgttaaata ccgtaaacct tcaggctgca tttcttacaa cattagaggt
    13441 ggcgcttgga attgccttac tcatgggatt gtggaaaaat ctggtgtcat ggttattatt
    13501 actgctgatt ttatttttca catggctcac aggttacagt gccataacag gcagtgtgac
    13561 cgattgtggt tgctttggcg attttataaa actcacgcca ttgcaatcct tctataaaga
    13621 ccttgttttg ttggcgttaa tattggtgat tttcttcttg cgcaaaggcc tgaaaccaat
    13681 aaagccactt ggaattagca taggtttgtt tttaacggtg accgtatttg ccatatgggt
    13741 gaatgtgcac gtattgcaac acgatgtatt cctcgactgg cgtccatatg ccgttggcga
    13801 taatattacc gagaatatga agttgccgga aaatgcacaa ccggatatta cggaaatgat
    13861 atatgcatac accaataaaa aatccggtga acaggtgaca ttgaaatatt taaataccga
    13921 gttgaaggaa aagaaaaatc tggatgaatt gctttcttac agtaataata aagatacctg
    13981 gaagctcgat acatcataca ccaaaataaa agtaaaaggt gatagggcta agattatcga
    14041 tttcgctatc agcgacgaac atgatgaatt tattaccgac caaattttgg gtaacccgga
    14101 tttcgccttg atggttgttt ctgcaggatt tgaacatacg aataaagagg gatgggaccg
    14161 catcagcgct gttcaaaagg atgctgagaa agaaggacta ttcacctttg cacttgttgg
    14221 cgaaggaaga acagagattg agaaattccg acacaatcag caaatggcat ttccgtttta
    14281 tactgccgat tataaggtat gtctcaccat cgctcgcgta aatccgaatg tagtactgat
    14341 gaaagaagga accatcattg caaaatgggc atggcgcgat cttccttcat tcgaggaaat
    14401 taaaaaaacg tatttccccg aaagaaagcc aaccgaatta atggtgattt caaatgagat
    14461 gtttgctccc ggtattcagg tgtcagcaaa attgcgggat gcagaagaac cctacaatga
    14521 attcttcctg caagatacgg ttggcaatga tctgactact gcactgatta acgatactac
    14581 aaatgctgtc atgtttatca tcaacgaact tagttccgat aaacttacca tggaaaattg
    14641 gaaatcaatt ttggctgtta tgcaaatgtc aaacacagcg caaaaaccat ttttcgttgc
    14701 cagttcagga tctcctgagg tattggagat gatgaaaaaa gcctctggat tggaatttgg
    14761 ttattatgtt tccgataaag atgtattggc aaaaatgatg gagacgaata ccggattaat
    14821 cgtaatcaaa aatggtaaag taacagccaa gtatgctgat ggtaaactac cttccgacag
    14881 caattttctg acccaataag aagtaatctt gattagctat atcatcaaac gacttggtta
    14941 cggcgtgctg gtgctgatcg gtgtggtgat agtggttttc tttctttttc agctgacacg
    15001 cattcacccg gagcgacaaa ttgcaggtga aaaagcagat aaagaaacaa tagaaaatat
    15061 caaaaaggaa ctaggacttg atctgccggt atcacaacaa ttattgttgt atttgaatga
    15121 tatttcgcct ttgagttttc atcaggttgc agatagtagt tcccgatatt ttttggatgg
    15181 cggtaagtat gattatttca gattgtttac tatcaatcat acagctacgg ttcttaagaa
    15241 accttatttg agcaggtctt acgtgagtaa gcgaagtgtg aatgaaattt tatcgcagcg
    15301 cattccgaat acattggttc tggcacttgc agccatgttg ctggctaccg tagtaggtat
    15361 tgccctcggt gtgattgccg ccgtaaaaca aggaaccgcg attgaccggt tatttactgc
    15421 cggaagtgtg ttgggtattt cttttccctc gtttttcgca ggcattatct tgcaattgat
    15481 atttgcttat ttgttggctt cgattacggg atttaatatg accggcaatc tgtacgaaac
    15541 agatccttat accggtgaac gttatctggc cttgcaaaat cttattctcc ctgccattgc
    15601 gctcggtatt cgtcctattg cggtaatcac ccagttaaca cgaagtgcca tgatagatgt
    15661 gttgagtaat gattatatcc gcaccgcaag agcaaaaggc ctttcagaaa gaaaaattgt
    15721 atttcgccac gcgctgaaaa atgccttaaa tcctgtagtt accagtattt caggttggct
    15781 ggcaggatta cttaccggcg catttttcat cgaggcgatt tttggataca atggcatcgg
    15841 ttatgaaaca gtgaatgcgc tgaaaacttt tgattaccct gttgccatgg gtgcaatttt
    15901 atatactgcc gtgatatttg ttgtcatcaa tattatcgtt gatattttat attctgtaat
    15961 tgaccctaga attcgcatcg catgagagtg tatctgattg gatttatggg ttgcggaaaa
    16021 acaactttcg gcaagaaatt ggctcggaaa atgaatgttg attttataga tctggataag
    16081 caaattgaag cgcatgaagg taaatctgta agtgaagtgt ttgccgaaaa aggggaggcc
    16141 tatttccgcg aattggaatc atcattatta cagcaaacta tatctcttaa tcatgccgtt
    16201 atctcttgtg gcggagggac accatgcttc catgataata tgcattggat gcgtgaacat
    16261 ggtattaccg tttatttgaa agttaccccg gaattccttt ttagcagatt gcatacacga
    16321 agagaaaagc gcccgcttat tgcaaaattg aatgatgagg aattacaagc atacatcacc
    16381 acaaaacttg aagagcgcga agtgcattat caacaagccc aacaagtcat tgatcctgtt
    16441 catgcaaaac ccggctttgt tgccgacctg ttgaatgcca gaaagggtaa ttgaaaatcc
    16501 ttacattaaa tatacacctg catgctgctt atcgctcagc acaactttga tgtgctcgtg
    16561 aatggtggtg ctcaatgata gtccgacata atcaggcgta acaggaaata atttgtgctt
    16621 gcgctctacc aatactacgc tgcggatttt tttcggtgaa taagataaca atggttttat
    16681 ggcgtacaac aatgtgcgac cggtattgga tacatcatcg gcaaggatga taaccttatt
    16741 gtctatctgc tcttcagtaa gatttaattc aacaacagaa tcacctggat tgtgtttatc
    16801 gagttcaaca tgcgttaata tgatatgtgc agatgatatt ttctttaatt cctgataaat
    16861 caattctgca aaagcataac cttcggtctc tttgcaaatg cctgccaata ctatttccgg
    16921 ttcatcgtaa ttatcttcag caatttcaaa ggcaatacgc tttatctttt gcaatacctt
    16981 ttccggagaa atgatctgag tgagtgtttg ttccattgat tatctggcta ctataaaggt
    17041 gtgtaatgtg taattgtcat gctcgttaat caagaggcga tacatgcctt cagcccaatc
    17101 gtgtatatca atttgattgg tttcggcatt tgttgttgtt tggttgtaaa tcacttgtcc
    17161 aagcatattt actatcacaa tgctgcttcc tgcatcaacg ccatcaacgt gcagtatatt
    17221 acttgccgga ttcggataca cattgatcag catttcggat gatatatctt cacgaagtgg
    17281 tggagtggta aatgtaatcg tggtgctgaa atcagagaat ccaaaacctg tgcagtaact
    17341 gcgcactttc gcattgtagg tagtatttgg cgttaaaccc gaaatagttt ttgtatggct
    17401 tggcgatttc aatttattcc atgagccacc gcttgattta taccaaatct gatatcctgc
    17461 agcatttggt ataatatccc aatctaacat ggctttagtt ggtgtggttg cgagtacctg
    17521 caaatcgaca ggcggttcgg tataatcatc tgcagcacaa ccccaatcgc cggtgagtgt
    17581 tctgaatgca tctgctttgc catatcccca taaattattc ggcaatgcag cgcctgtgaa
    17641 gtcatctttt cttgcttgag aaagaatggc atcttttatt tcagccgcgt tggcgtttgg
    17701 atttttttgc agatataatg cagcaattcc ggcaacagct ggtgaactga atgaagtgcc
    17761 attatataaa taatgctgag catcggcact tacataagtt gcggcatcta acaatgccaa
    17821 ccattctgta agtgtatcac ttgccgtgct taaaacgcgt gctccgggag cgcagatatc
    17881 aggtttaatg cgaccatcgc gtgtggggcc atgacttgaa gaataaaaca gggcacctac
    17941 tacatcgaaa aatggcgggt tctccaaata aaaattcgtc atgctatcgc gattcacata
    18001 actgcctacc gtgattacat gatcgctgca ttggaagctg ctcacaatag tttgatccgt
    18061 atccggtaaa cgataattga caatctccgg taaaacaaca gcagaaggta aaccggtggt
    18121 aacataattt gaaaaacctg ttgttgattc agccgaccaa atatcaaaat ggccgcttcc
    18181 tactgtactc aatcgccagt aatttccggg gactgaaggc gttgccacaa attccaggaa
    18241 atatttatcg ttatccaact gcgcatatat ggtgatattg ccgcctccgg gaatggaata
    18301 tgtaactgaa tcaatgatat cgccggtgaa atcaaaatcg ctcagcatat tgaaataggg
    18361 ggtacttccc aaagatgtaa atccggcagg ctcatcaaca cttaagcgaa aatctacctg
    18421 attaaaatca gtggtatctg cccacaactg aaaataaacc aggttcatgt aactcaattt
    18481 cttaaaccag gtgaattgct cagtggcagt aacatcgtac cccaggtgga aggcattatt
    18541 ccctgcattc cctgcagcag caacaagtgc gcgtcccgga gaggctgtta tcaaggcatc
    18601 tatggtctga ctcatcaggt cggaaccatc gtgagaaccg gcataagagc cgaaagaggt
    18661 attaataacg cagggtttcc ccatttcagc agctttggca tagatataag ctatggcatc
    18721 tgttgcgttg gtttcataat cattgttaaa atccattgaa accaagatca ggtcagcatt
    18781 tggagctact ccgcggtagt ggttcgatgc catgccgctg gatgccgcaa ccccggtaac
    18841 gccgcttccg tgcgaataat gtaaagggtc atcatccacg cttggacagg tgccatcagc
    18901 aatcatagcg ctgtcgcact caacaccata tccataaggg gtaggagggg ctccggttgt
    18961 gatattctgg tcccaaacgt atttaatgcg actattgccg tcggcatcgg taaagtcgcc
    19021 atggtcaagg tcaaaagggg catctatgat acctaccaca actcccgtgc catcgtaggc
    19081 ctgttccaga ggccaatagc cgtaaaaggc cgaatctacg ttgttgtgga gcaccatcac
    19141 atcattcagc aggttgcctt tattggcatt ggcctcaatc ctgactgcca ggccactttc
    19201 attgatagcc ctgatcgcag cgcggtctaa agtagccgaa atgatatctc cggcagcaaa
    19261 cttaaaggtg ccgccatact gtttgatgag tgctgcaaga gccggcacat cgcccttgat
    19321 gtagacaggg atttgttcac cgggagccgc atcattcatg gcatgatagg ctgaaagccc
    19381 gatacagctg ccgggggaag attgttgcgc atctgccgaa aaacatattg cggctaacaa
    19441 tactatgagt aaggttttag gcatgtgtat aatccctttt tacagtccta aaaatacgct
    19501 taatttagca accgtataaa caagtattgc atggagatag ttctgcaggt gcttttcgca
    19561 gtggtgtttg gtgtgtcctt ctatttggcg gggagaaaat tcgttgccat ccgcaggaat
    19621 atattattgg ggcgaaaaga atcgcttaca gatcagccgg ccagaagatg gaagcatatg
    19681 gtgcttttcg cacttggaca aaagaaaatg tttcgcaagc cgcttcctgc cgtattacac
    19741 ctatttatct atgtgagttt tgtaatcgtc aacctcgaat taattgagat tgcaatcgat
    19801 ggcgtaaccg gcaatcatag aactttcgca ccgtatctgg gtggatttta cacttttgtt
    19861 attagcctga tcgaaacatt atcggcattg gcattagtgg caacgcttgt ttttctatgg
    19921 agaagaaatg tgctcaaggt aaagcgattt acgcagcccg aattaaaagg ctggccgttt
    19981 aaagatgcca attttatttt gggaatggag ctgatattag ttacggcaat tctcaccatg
    20041 aatggagccg attaccgcct tcaacaatta ggtgcagcgc attatgtaga tacaggcacc
    20101 ttattggttg gtcaattcac cagccaactt tttatgcata tgggcactgc atcattagag
    20161 catgttgaac gtattgcatg gtgggtacat attctgggta ttttattctt cctgaattac
    20221 attccttatt cgaaacatct tcacattatt ttagcattcc ccaatgctta ttataccaga
    20281 tttgctgtgg cgggaaaaat ggaaaacatg cctgtgattg ccaacgaggt gaaatcgatg
    20341 ctagacccct ctgcagcgat aactgaaata cctgatccgc caaaaacctt tggtgccaaa
    20401 gatgtaaccg acctcacctg gaaacatttg ctcgatgctt atgcctgcac cgaatgtgga
    20461 agatgcactg ctgcctgtcc ggcaaatatt accggaaaac aattatctcc tcgtaagata
    20521 atgatggata cgcgcgacag gctggaagaa gtgggtcgta attttgacaa gcatggcgct
    20581 attaccgacg atggaaaatc attgtttggc gattatatca ccgcagaaga aattcgcgca
    20641 tgcaccacct gtaacgcctg tgtggaggaa tgtcctgtgg gtattcaacc attgagcatc
    20701 attctggagt tgcgccgtta tgccgttatg gaacaaagta atgcgcctaa tgagtggaat
    20761 atgatgttca ataatatgga aaataatcag gccccatggc aatttaatcc gggcgatagg
    20821 gtgaactgga aaaatgaaat ttaatctgta agcaagcttt acacagtaag ctaaatgaat
    20881 atgcaggaga atctgactaa tgtgccgata atggccgaat tatttgcaaa tggcgaatca
    20941 ccggaagtgc tgttttgggt tggatgtgcc ggtagtttcg atgatagagc gaaaaaaata
    21001 accggagcat ttgtaagaat tttaaacagc ataggcgttc gttttgccgt gctaggaaca
    21061 gaagagtcat gcaccggaga tcctgcccgc cgagcaggta atgaattttt gtttcagatg
    21121 ctggcgcaaa acaatattgc ggttatgaat ggctatggta ttaaaaaaat tgttaccacc
    21181 tgtccgcatt gcttcaatat ccttaaaaat gaataccctg cgcttggtgg tgaatatgaa
    21241 gttcttcatc atacggcatt cctgcaacaa atgatagatg aaggacgcct gaaagtggaa
    21301 ggaggtgcat ttgcaggtaa gcgaattact taccatgatt catgttatct cggaagagca
    21361 aatgatattt acgaagcgcc cagaaatgta ttaattcaac tcgacgcgca attggcagaa
    21421 atgaaaagct gtaaatcgcg aggtctttgc tgtggtgccg gtggtgcgca aatgtggaag
    21481 gaagcggaac atggcaataa agaaataaat atcgaacgta cggagcaggc tttggaagta
    21541 aatccggata tcattgcatc tgcttgccct ttctgcaata ccatgatgac agatggcgtt
    21601 aaaatgaaag aaaaagaaga aggggtcaaa gtttttgatg tagccgaatt agtagctatg
    21661 gcaaacggat tattcgcatg atagcaacag aaaacaatag catagaaaag cttatcgctg
    21721 attttgatcc tgcttcaaaa gtatgggtat atcaatcttc aagacccttc acatcggctg
    21781 aggagaatga aatctcacag gccttgcaat ccttcaccaa acagtgggcg gcgcataata
    21841 ttcaattgaa agcaacaggt tttatcgttg aatcacgcgt gatcgtttta atggttgatg
    21901 aaacgcatac cgacgccagc ggatgttcta ttgacacctc ggtgcacttt attcaaggtt
    21961 tggaaaatga gtatggtgtt gaattgtttg atagaatgat agtgcattat cgcgatgtgg
    22021 agggatggca tactaccaca ttatctacct tgcccgattt aatttcgcag catcttataa
    22081 gtaaatcaac aatcgtaata aatccattgg tagcaacaaa agaggaatat caacactcat
    22141 ttgttacaag gattgaagat tgctggatga tgcaattcgt ataatattta gcatacttag
    22201 tcttacactc tctacgttat tcatattatg aaaaccctcc tcacgcttgt attttgtaca
    22261 ttcctgtccc ttgcatcgtt tgcacaggca acagattcca cgaaaaccga ttctattaat
    22321 tcggcatact tgaatttata tctggatggc ggatacggct atcaggatta tatcaaaaca
    22381 gaaatacctt ttgtaaacta tgtgcgcgac agatttgatg gtgatgtgca tttgttgatt
    22441 accgcccaag gtaccggaag tggtggtgcc gattatactt tgtttttcct tggacagaaa
    22501 acatttgaag gaaaaaacga tacgctgcat tatatcgcga ataccaatgc caccagcgaa
    22561 gaaattcgtg ccggattagt gcaggttatt aagatggggt tgttgccatt tattgcaaaa
    22621 atgaatgccg cctttccggt gcaaattaca ttcacaggca gtacaaacag cgaagagcga
    22681 caagaaaatg ataagtggaa gggatgggtg tattctatat cgtcatacgc taatgccagc
    22741 cttacctcca actatgaatc ttattcaatt tcaggaaatg tgtcggccga caaggtgacg
    22801 gaggaatgga aactcggatt ctcatacgga gcgaattata ataccgatgt gtttgatttt
    22861 ggtgatggcg acatttatga aagttttaat aatagcacct atggaaacgc aaccattgtt
    22921 aatagtttaa atgatcattg gagtttaggt ggggaagggt tttatagcac gagcacctac
    22981 agcaattata aaacaaatat tgccattgct cccggaatag aatatgatgt ttttccttat
    23041 gacgagtcta ccaccaagtt gctaaccttt ttttacaaag tgggacctga gtatcatcat
    23101 tatatcgact caacaattta caataagact gaagaattat taatgcgcca gcgattggat
    23161 gtgtctttgt cgctcacaca aaagtggggt tctattactg tcggcatcaa tggctctaat
    23221 tatttccacg acctcaccaa gaatggcgca ggctgtttta catctataaa ctggaggata
    23281 gtggaaggct tgtcgttaaa cggatttttc acacttgatt tcatcaatga tcaactgaac
    23341 atccctaaag gcgatctcac ggatgaggaa atcttattgc aaataaccga gcgtcaaaca
    23401 aattatagct tctattcctt ctttgggcta tcatatactt ttggctccat ttacaacaat
    23461 gttgtaaacc cgcgttttag cggaggctcg tatagttttt atttcagtaa ttgatgagga
    23521 tgcttatatt ggatattgtt aaagcgtaat tgaatttgtt atgtatacac caagtttctt
    23581 gtatatacat aagcagcgtt cgatgcatga taactattat gtttttgaca ccattatggg
    23641 cgccaagtat cccttttggt atatggtgat attatttatt ttccaaatgg gcgcccttgt
    23701 gaaggataat tgggtgctga cggtagcatt tcttcccatg tttatcattt ttgccatgta
    23761 tgaaggtgtt gaagtaaata ttgcgaagat gcagtatcgt gattacacgc atttgtttca
    23821 atggtggtgg gggaggttta agccaattga agaccctgat tacttactcc tgtcgagaac
    23881 atcagacacc cgcaatggcg gcattcgcta tgatgtccac ttgattacaa aaggcagtcg
    23941 cgatttacta ttatttcgca ccaaaaacct tgaggaccat gaaaacgttg tcaaagtatt
    24001 agcgcagcgc accggttgga aggcatatag gaatactatt gatgggttgg aggggtttga
    24061 atgag
//
LOCUS       DAOQHY010000028        23858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_931_length_23858_cov_5.263244, whole genome shotgun sequence.
ACCESSION   DAOQHY010000028 DAOQHY010000000
VERSION     DAOQHY010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 23858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23858
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_931_length_23858_cov_5.263244"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..1324
                     /locus_tag="PLL28_03895"
     CDS             227..1324
                     /locus_tag="PLL28_03895"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNF68491.1"
                     /translation="MSNNTLQLTNGEVFTTMREMEHEQLVFCTDNNTGLKAIVAIHNT
                     TLGPALGGTRFWSYQQEDDAIIDVLRLSRGMTYKAAISGLNLGGGKAVIIGDSKALTN
                     REPLFRRYGKFVNGLAGRYITAEDVGTSTSDMEFINMETDHVAGKPEYLGGGGDPSPV
                     TAYGVYLGMKSATKYATGSDNLSGKRILVQGVGHVGYNLVKHLTKDGAKVMISDISEE
                     RLKAVAAEFGAEIIGTDKVFDTQMDIYAPCALGATINDDTINKLSCQIIAGAANNQLK
                     DEAVHGKRLIERGITYAPDYLINAGGLINCYVELEGYNRERALSKTEIIYDRAMEIFQ
                     VAAKNNITTQAAANQVAEERIRAIANINARL"
     gene            1372..2310
                     /gene="nusB"
                     /locus_tag="PLL28_03900"
     CDS             1372..2310
                     /gene="nusB"
                     /locus_tag="PLL28_03900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNF68492.1"
                     /translation="MLSRRGLRIKAMQTLYVAACSGQPEPKAALSHLSKSIRNSHLAY
                     LYILHFLTELAQQVDLEAHIKKNKYLPSAEDLAFPTTFFDNKVIQGLLKNNYFQSRLK
                     REKLTDLVDEEYSKIIYQQLKEYAPYNNYVGSSPDLVADKKMLKDIFEHFLLKNEYFD
                     EHMENSIATWADDEFIVKGLMDEFFNNNISEQTEKHLFDFQVGDEEMAFAESLVVFTM
                     ESDLQYDEEIKPKLQNWELDRVSLIDRILMKMALTEFLHLPTVPTKVTINEYLDISKL
                     YSTPRSREFINGILDKLMNEMKSSGRIVKTGRGLIE"
     gene            2326..2787
                     /locus_tag="PLL28_03905"
     CDS             2326..2787
                     /locus_tag="PLL28_03905"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNF68493.1"
                     /translation="MKKQVLFASFALIFGAGILLSCKEQSPVDVKAKSGSDEKLNADL
                     VKNQTSITFERDMHDFGEIIKGEKVSTEFKFMNTGKNDLLISDAHGSCGCTVPEWPKE
                     PIPPGGSGVIKVVFNSGNRSGEFNKTVTVIANTVTNPNKLIIKGTIITPTN"
     gene            2833..3147
                     /gene="yajC"
                     /locus_tag="PLL28_03910"
     CDS             2833..3147
                     /gene="yajC"
                     /locus_tag="PLL28_03910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNF68494.1"
                     /translation="MAPAADGQSSGSSWVQIAFFFLFFLILYFFMIRPQSKKAKEQKN
                     FLNEIKVGDKIVTIGGVHAKILKIDDDTYLIEVDTNTKLRIEKSVISLDFTRAMLARK
                     QG"
     gene            3154..4059
                     /locus_tag="PLL28_03915"
     CDS             3154..4059
                     /locus_tag="PLL28_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68495.1"
                     /translation="METMGDSTPAAQRSFAGEFALFFVCLFIAVIIWLFTALSENYEQ
                     EINVGLSYVLPESQTTTIPLPQEAHLLVSSSGWQLVKEGFRHRAMSLDVSDLDDKPVL
                     LTNNHLGLFTAGLPGNMHIVQVIPDTIDLHLEPLAERKVPIVLNADFMDRTDIVVDSI
                     YIHPDSITIRGPKSLITDITYWSTEAIAGNGDSIIDGITPLPFPAVGSIRLSAILVDW
                     HAEIEAAIEANAYLTINTPTNHAVDVRIQYSSAAEVSSLISAKDFNITFEKTADGTQY
                     HIVARAKRKGLHTIGVYPAFIPITR"
     gene            4061..4678
                     /gene="coaE"
                     /locus_tag="PLL28_03920"
     CDS             4061..4678
                     /gene="coaE"
                     /locus_tag="PLL28_03920"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793821.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNF68496.1"
                     /translation="MIKVGITGGIGSGKSTVCKIFEVLGVPVYYADERAKEILQTDAE
                     IIKRVKDLFGEDVYDSNHVLDRKRVAFVVFHEPEFLAQYNKIIHPAVLLDSEKWMQRH
                     LQYPYVIKEAALLFESGGNKQLDKVICVTAPEDLRIQRVMQRDNTTEEQVRSRMANQM
                     SEEEKLEKSDFILYNDGSAPLIRQVLNIHEKLLEIIRKQDQLEKR"
     gene            complement(4682..5533)
                     /locus_tag="PLL28_03925"
     CDS             complement(4682..5533)
                     /locus_tag="PLL28_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68497.1"
                     /translation="MFLTVLSCQAQIWTLEKNGVQASASYTWFHYDKAYQDNSTTIDL
                     PLTVFDKTLALDVQYGITDRITALARLPYKMLETTGYPGVYNSYPGQYIETGKLNNIG
                     NTEFGLVWKCYDDKPIVSASFIVEANTSDRNYITGLQTGFNSWAFRPGIGVGWGFDKS
                     WLQFYTGMSIRTNDYALAVVSNLEAGYHFADYWWGAISLDTRKPNVDGEDCDCSTSAT
                     MLYQNAQEYYALALKTGFTMYKDWGLNFGYHLGLTGNDVAATAVPVIGVMYKSRPMRH
                     TIITPRF"
     gene            5612..5827
                     /locus_tag="PLL28_03930"
     CDS             5612..5827
                     /locus_tag="PLL28_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68498.1"
                     /translation="MTASTFTLLIDILTLLFMIVGALWMWGVFKFKAEVQQRFEEIFA
                     RSRKRFKWMFLIGAILLAALFVRDYFL"
     gene            complement(5824..6930)
                     /locus_tag="PLL28_03935"
     CDS             complement(5824..6930)
                     /locus_tag="PLL28_03935"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNF68499.1"
                     /translation="MAFGINSRRIKKAIRRLKSLPRGANFFRYTSNKVYGAYLRMIHS
                     TKVAHPSSIMLEVTNHCNLKCITCPREYRYGEEMDKGFMDIAQLKKVIDQAYPYIDSV
                     GLTGLGETLLYKQLPEALAYIRSKNKGIITTISINAHLPNSVELVEKIADDLDTLQIS
                     MDGVGEVYEQVRLRGEFSLFYDNVKRIVELCKNRRADVMFNFVAIKENYFNMAEVVEV
                     ASELGVDYINVTPFNVAAVTAHDISYYDFFHSDAFKNELKRAKDKADEVKNVTLTIWD
                     IKTPNTYQKCHLPWSHFYISWDGYATPCCAKPFPKELNFGNIFDDGLMYCLNTPGYRE
                     FRRLWYENKTPQFCEKCHMVDLTPIDLPFELPVN"
     gene            complement(6966..8519)
                     /gene="hemC"
                     /locus_tag="PLL28_03940"
     CDS             complement(6966..8519)
                     /gene="hemC"
                     /locus_tag="PLL28_03940"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNF68500.1"
                     /translation="MKHKVIIGSRGSDLALWQANYLKKSLLEIGVDADILIIKTQGDQ
                     IQHVSLEKLEGKGFFTKEIEDALLENKIDIAVHSHKDLPTATTPGLKIAAVSYREDPS
                     ELLLVRKEIADKTQFWNLPLHAKVGTSSSRRAVQLHFYRPDIQLADIRGNVPTRVQKL
                     RNGQYDAILLAAAGVQRLQLLLDDLLAIKISPHHFIPAPAQGVLAYQCREQDIEVLEI
                     LEKLHHQSVYDTISVERKVMQMFNGGCHMPLGVYCQYMHGEFFVWATQSEDKLNTPRR
                     LFVRSKNNHNLAEVIYDKLRNRSDKKVLFTTEPDAAEQAIKIVEAAGHSVIAQSYIKI
                     VPKYFDFNIDCDWLFFTSKNGVNEFFKVVKQMPSSIKIAAFGAETAEKITTFGYDVAF
                     TGNGLVEDVAKQFYEHGKTMHVVFPCAVNARKELHEAIAPYAHVFALPVYENILISNE
                     IFEADIIACSSPMQAEGYLKQHTPDGKTFIAIGPTTARYLENIGISNVHVAPFPSLIS
                     MAELICGLV"
     gene            complement(8519..9745)
                     /gene="hemA"
                     /locus_tag="PLL28_03945"
     CDS             complement(8519..9745)
                     /gene="hemA"
                     /locus_tag="PLL28_03945"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNF68501.1"
                     /translation="MNQYKVLTVTHKQVPLGQLSNYIVPFDKQTTNYSQQLQSIKQEM
                     KLDELMYLNTCNRVTYFFTTTQPVDNQFTAGFFKTVNPEFEACNVMHSQLFEGVEAIE
                     HMCEVSASLDSLVVGEREIIRQLRVAYEESNEMALTGDDIRLAMKMLIPAAKKIYTET
                     HIAEKPVSVVSLAALRLRELEIPQHAAIILIGAGETITNMSAYLADMGFQNFSVFNRT
                     LANAEKLVSSIGGKAYQLDALKTFTGGFDVIISCTGSVEPVINEEIYQQLIGRDTQKK
                     VIIDLAIPQDIDTTILSRNQIDYIHIDDLRSIAQKNLSLRKAEVVKAKKLVHEFVKEF
                     QAAYIERLVEKAHSAIPQKLHEIRLRAVEEVYAKDVASLDPHAKEVMDKLLDYMEKKY
                     LALTMASSKSNLRHPH"
     gene            complement(9871..10323)
                     /locus_tag="PLL28_03950"
     CDS             complement(9871..10323)
                     /locus_tag="PLL28_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68502.1"
                     /translation="MRTSAYIIIACMLLSACKKDEEINPPISEVPEIELMSVSPGTVV
                     ALEDSIVFLIHYIDGDGDLGYNEPDSMSLYITDNRIPLTESFFVPWLAPEGSEITIEG
                     DLEVQLHNTILVDPDSDSETVTFTVWMRDRAGNLSNSVTTGPITVTRD"
     gene            complement(10326..11306)
                     /locus_tag="PLL28_03955"
     CDS             complement(10326..11306)
                     /locus_tag="PLL28_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68503.1"
                     /translation="MKQFPITKIAVMSMLCAFTLIACREIIRDEPDLPDNPYDDVDYG
                     NGGTDPVLIDSASFLGLHTFIFSTTCAVPGCHDGTFEPDFRTVQSAYNSLVYAPVIKN
                     DDDDTFTYRVVPGDTALSWLHERITTDDAVLGRMPLYDTLYPHEREMITQWILNGAPD
                     IFGNSPILPDYQPSTYGYICYENDTTGLRLDTNRADLLQPVILPQHSTVEYWFGIYDT
                     DESGVYQLPSWLEQYNIRISQDMLMNTGFTEYTFELEPDFDPYYGPAYYDPGIPTPYW
                     HHFTLNTDDFETGKLYFMRLYLKDTAHDFVTELPDDSQIYIAYYFTFRIE"
     gene            complement(11303..12631)
                     /locus_tag="PLL28_03960"
     CDS             complement(11303..12631)
                     /locus_tag="PLL28_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68504.1"
                     /translation="MNRRKFIRNSLLTTAGMIAAPYILPAGTLSRSSGMRLANHVVLV
                     VFGGGIRNQESVSQQYLLNQAAGPGGNIMPNMLSGAQPTYNLVYDQWAPALASPLATK
                     GTLFKHMRYENGPTGHFNGHTAVITGNYTPDSLNLSANPAMPTLFEYYRKHNDPLKSA
                     INAWWMSIDLGPYVYLNYSKDPLYGANYGANYMSPITTFGNIGVDYFSTLNTMHPDEI
                     TRMNAVRSFLDANFDRSVLDVPGIRNSEEDKQAIEDFMLGLLHGEIPIEWPLPAGVGF
                     DQMTGDLTNIAASWKVMQQFQPELMVINTTNLDICHTNFSSYISYLHRADYGIGWLWD
                     KIQSDPILANDTVMICVPEHGRNTVANTIPDANGLFAYDHTGDENSRDIFCLVVGPPS
                     IVNQGLVVGSEEEPVGQTIDVVPTIAHILGFKDNIPSGYLPGTVLEQAFV"
     gene            complement(12646..13170)
                     /locus_tag="PLL28_03965"
     CDS             complement(12646..13170)
                     /locus_tag="PLL28_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68505.1"
                     /translation="MQASKLFSTLGILGVVLLSNSCKKDVIQEVVYDNIIYGVDTVPV
                     YQSNEEKDRVKTPLQYISGLYSNLYFSSIPGNILDNLVIYRLSIGDKTLVNELILNAM
                     LQDPAVLANIPSDADMRGDVDQFVEDCYLRFYLRKPTAYEQYALSDMIELDADMTVVD
                     VYRAFLLSNEYMFY"
     gene            complement(13157..14044)
                     /locus_tag="PLL28_03970"
     CDS             complement(13157..14044)
                     /locus_tag="PLL28_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68506.1"
                     /translation="MRQGLILISSVYLLAVSCAKDEVIHENLIIDGNIAPPVTGVSEI
                     ALNNYINSLYIDLFGRTATADELSEHAETLQSEQYSIEAREGLVNSLLSDWEHINNIH
                     LTTGQKFLVDVDSFAIASQIAYWDYLIDLFTDMGMEGEAGYYIFENDRLIKLITAHID
                     LANGTIGADEFYRRYINNYFYDQVNMGSLNFVVSCFTNLMQRYPTDDEQESGINMVDG
                     VSDVLFLQDGSSKGDFIDIVTSTPEFFQGLVIESFNTYLARDPNSLEMDTYTQQMMLE
                     NNVNGVALIILTSTEYAGF"
     gene            complement(14398..15060)
                     /locus_tag="PLL28_03975"
     CDS             complement(14398..15060)
                     /locus_tag="PLL28_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNF68507.1"
                     /translation="MMKKIHLAFLAISIPIFAQAQFTEGDWLLGGSLLLSNTTSNSSG
                     TGYDDKGPSSSTIYAGPAVGYFIGDKWMIGLRAAFNNSITTIYHPDDPALDMHFTRSS
                     VGGGPVARYYPFQSQYVDLWLGAGAYVGFGKNSDESIVFDDNGVGSINEQTDNLINYS
                     LGIGPGASIIFNDHWRLELACGSLGFSGYQTKDKTTDAVFTQQSISLSLGSIGFGIAY
                     KF"
     gene            complement(15075..16043)
                     /locus_tag="PLL28_03980"
     CDS             complement(15075..16043)
                     /locus_tag="PLL28_03980"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HNF68508.1"
                     /translation="MLFRTNKSKIIGVISFGILLMSFYSCTTDITLDIPDPEPQIVVE
                     GSIENGVPPIISLTSTIPFYGEVNFNELENYYVHDAVVTVSDGVNTVELVEYCLSELP
                     DEILPLVAEYLGVELDTAGELAIDVCLYTVADIFTGSPSFVGEEGKTYDLHIEANGQT
                     ITSSTRLLPAVPLDTMYFEPHPDPENDSLMRLYVTLSEPEPQGDFYRYFTRRNSDPFY
                     SGYSSVFDDNLINGQTFDFTLDRGYDPTEDYSMGTYGYFWKGDTVTLKWCHIDFPTYD
                     FWRTLEFDSGTDGPFSSATIAQTNIVGGLGIWCAYGVSYTTQIIPE"
     gene            complement(16071..18437)
                     /locus_tag="PLL28_03985"
     CDS             complement(16071..18437)
                     /locus_tag="PLL28_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF68509.1"
                     /translation="MKTGLFGLLMCACSMLFAQGKYTLSGYVKDEGTGEDMIGANVYL
                     QNDPGTGASTNGYGFFSMTLPEGDYNVVISYVGYDNQIEKVSLHNDVRLNVQLSQGKL
                     LEEVVVKAERGDVNIQSTDMGKVELNIEQIKTIPVLFGEVDILKTIQLLPGVGGAGEG
                     NTGFYVRGGGPDQNLILLDEAVVYNTGHLFGFFSVFNSDAIKNVTLVKGGMPSQYGGR
                     LSSVLDVQMKEGNNQYYQAEGGIGLIASRLTIQGPIVKDKSSFIVSGRRTYIDALITP
                     FLNGTDFEGNGYYFYDLNTKVNYTFDDKNRIFASGYFGQDVFNYSAPDGSLNVSVPWG
                     NATGTVRWNHLFNNKLFMNVSAIYNSFNFSVQSDFQDFNFNLYSGIQDWNGKVDFDYY
                     PGSNHHIKFGANYVYHTFTPYSAQGSFDDVDFSTDTLSNKYAHETGIFIQDEFDVTDR
                     LKINIGLRGSYFAQVGPYNDIVYDENGATVIDTIYYAPGEMIAHYSGLEPRFNMRFTI
                     DPESSLKAGITYNNQYIHLVSSSTSTLPTDLWIPSSDIVKPQIGIQYALGYFRNFKDN
                     AYETSIEVYYKDLRNQIEFRDGYVPELGLDIEDNFVFGVGRSYGAEIFLKKAKGKFNG
                     WIGYTLSKTERKFEDLSTGSEWFPTKYDRVHDLEVVAVYNLSDRWDFSSTFVYATGQA
                     TTIVESFYVIGGQVQTDYGPRNGYRLPAYHRLDVSATLHSKPERKYESSWNFSIYNVY
                     SRKNPYFIYTTYEGDFAYGYLDVQAKQVSIFPIIPSVTWNFKFAQQHK"
     gene            complement(18548..20143)
                     /locus_tag="PLL28_03990"
     CDS             complement(18548..20143)
                     /locus_tag="PLL28_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNF68510.1"
                     /translation="MMHEVSFAQSQNIWVYFSDKNATPYTIDNPSAFLSEQAIDRRER
                     MLIPIRKSDLPVDPQYISAIKQTGALVRTESRWLNAVSVTVTGPEMIAEISALSFVKQ
                     ITPVIKRHLIDDVSVPQDQAFYRTADITDFVYGGAQNQNHMIEVDFLHSLGFRGQGIT
                     IAVLDGGFEGVNTGAGFESVRAKEQIKGTYNFPDDTDFVYFSSTHGSNVLSIMAVDEP
                     GVYVGSAPDADYYLIRTEVTDSEHVVEEDYWLEGAEYADIIGADVINSSLGYTTFDDT
                     TEDHTYLDMDGNTTVATIAADMAAAAGILVVNSAGNSGDEPWHFIGAPADGDSVFTIG
                     AVDLNGNYAGFSSVGPTVDGRIKPNVVGQGAGTAVMSAEGIVGYGNGTSYSSPLIAGA
                     CASFMSAFPDLSNMDVINIIQQTATNAGTPNNQIGYGIPQFGHAYMALKGITLNDDIL
                     HVYPNPVSDVLVVYIQGFEADQAKFDMFDMAGKMIMSTDLLIDAGKMSGIRIEDLDRL
                     ATGLYTIRLTGDIYSESKLIYIQ"
     gene            complement(20194..21891)
                     /locus_tag="PLL28_03995"
     CDS             complement(20194..21891)
                     /locus_tag="PLL28_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNF68511.1"
                     /translation="MQRFLLICLICCLSITSFAQQHTDRYWVYFTDKQFNTFSKQSPE
                     QFLSKASLNRREKQHIPISESDLPVSEYYIQSLENIGVQVYVRSKWLNAVSVIMSDDQ
                     MLQVSELPFVNKISRVGRYQILNEQGTPLNDQTFYRKADNTEITGGLGAASNQIEQIN
                     LDTLFDIGLSGDSMVIAVLDAGFFGVDTATLFQSFWDKDQILGYHNFPDNNDQVFNIY
                     SGYHGSWVMSVIAGEVTDLFSGSAPNADLYLYRTEIADSEYVVEEDHWLAAAERADSV
                     GADIINSSLGYTVFDDPTQNHTYEDLDGNTTVVTMAADMAASKGILVCNSAGNEGAGA
                     WHYVSMPSDGDSVLSVGGVDTSGIHVGFSGYGPTFDGRLKPNVVALALGSAVLDPNGY
                     INYLSGTSFSSPLLAGACATLWQAYPEATNMQILHAVEQSAHLYDTPNDSMGYGIPNF
                     GKAYALLDSLFPEIDTTVVDTTVDTTIDTTLSSIAIIPLLDGDIFTVILQSLTNREAT
                     FAVYNLQGALMHQENVYLNAEMPTTVEMGNKHLYASGIYFLTVRSGRYKQSVKVFIE"
     gene            complement(21898..22653)
                     /locus_tag="PLL28_04000"
     CDS             complement(21898..22653)
                     /locus_tag="PLL28_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68512.1"
                     /translation="MFSRSITGLILTAFIFAASMGCDKPLPEPNTPETPYAYSPLKIG
                     NYWEYAVDSIIYHETTGNDTTLWFVREELIDTFYDLTGDLNYVIDRYRRKTDVDAWQL
                     TNTWSVMQVDGKMIRNENNLRFVKLVSPPLNDVQWDGNVYLGGLDDLPYDEECNRLIY
                     YEGWDYIYSNVNENYSIPDFDFENTITVEQFGDSNLIWFDYAKEIYALDVGMVEKTFK
                     HYYTQDITCPDCPWEQRVQCGFSVYMRLTDYQL"
     gene            complement(22801..>23858)
                     /gene="mnmA"
                     /locus_tag="PLL28_04005"
     CDS             complement(22801..>23858)
                     /gene="mnmA"
                     /locus_tag="PLL28_04005"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNF68513.1"
                     /translation="VTAIMLHEQGYEVVGITMKTWDYASSGGSKKETGCCSLDSINDA
                     RMVAVDKGFHHFIVDIREEFGDYVIDNFVDEYLAGRTPNPCVLCNTHIKWEALLKRAD
                     QLGCEFIATGHYASVHQNPDNNRYFISKGKDENKDQSYVLWGLSQDCLCRTRFPLGQL
                     HKTEIRQMARDMGYTELANKAESYEICFVPDNDYRGFLKRRVDGLEEKVKGGKFVDKH
                     GKVLGTHQGYPFYTIGQRKGLGIALGEPYFVTEIIPHENVVVLGLEEELNKNGMFVHH
                     LNMLKYENINEPMRAVTKIRYKNAGAESTLFSEPGDLLRIAFDSNVSAITPGQSAVFY
                     EGNDVIGGGHIVSAFQQ"
BASE COUNT         7044 a         4908 c         4887 g         7019 t
ORIGIN      
        1 tataaagtgc ggagcatata cctgaaacag gtttgcgaga cctacaaaca aagttccggt
       61 gaggaacagc catttgtatt tgagcaggta ttggtgaacc gggcgaaggg cattcatgca
      121 ggtgcaaaac tatatattac ccaaaaaggt tttccactaa tagaacatct atttcttttt
      181 acaacttatc ctatctttgc agcgccaacc aaaaaccgtt cattatatgt caaacaatac
      241 cctgcagcta accaacggag aggtgtttac caccatgcgt gagatggaac acgaacaatt
      301 ggttttctgc acagacaata acacagggct gaaagccata gtcgctattc acaatactac
      361 attaggccct gctttgggtg gtacacgttt ctggagttat cagcaagaag atgatgcgat
      421 tattgatgtt ttacgccttt cgcgtgggat gacctataaa gcagcaatca gcgggttgaa
      481 cctcggaggt ggcaaagccg tgataattgg cgattcaaaa gcgcttacca atcgcgaacc
      541 tttgtttcgt cgctatggta agttcgtgaa tggacttgcg ggaagatata ttactgctga
      601 agatgtaggt acatcgacat cagacatgga gttcattaat atggaaacag accatgtagc
      661 aggaaaacct gaatatttgg gtggaggtgg cgatccgtcg cctgtgactg cttacggtgt
      721 ttatctggga atgaaatcag caaccaaata tgctacggga agtgacaatt tatccggaaa
      781 gcgcattttg gttcagggcg taggtcatgt gggatacaac cttgtgaaac acctgacaaa
      841 agacggtgcc aaggtaatga tcagtgatat cagcgaagag cgattaaaag ctgttgcggc
      901 tgaatttggt gcagaaatta ttggaacgga caaggtattt gatacccaaa tggatattta
      961 tgcaccttgt gctttgggag ccactattaa cgacgatacc atcaataaat tgagttgtca
     1021 gattattgcc ggtgcagcga ataaccaact taaggacgaa gctgttcatg gcaagcgatt
     1081 gattgaaaga ggtattacct atgcgccgga ttatttgatc aatgcaggcg gactcatcaa
     1141 ctgttatgtt gaattggaag gatacaaccg cgagcgagca ttgtctaaaa cagagatcat
     1201 ttacgatcgc gcgatggaga ttttccaggt tgctgcgaaa aataatatta ccactcaagc
     1261 tgccgccaac caggttgctg aggaacgcat tcgtgcaata gcgaacatca atgcacgtct
     1321 ataagcaggg acttgcagta ttacccctta aaaaacactg cgtaatttga catgttgagt
     1381 agaagaggat taaggataaa agcaatgcag acattatatg tagctgcatg ttccggtcag
     1441 ccggagccta aggctgcatt gtctcatttg agtaagagta tacgcaattc gcatcttgct
     1501 tatttatata ttttgcattt cctgactgag ttggcgcagc aagttgattt ggaggcacat
     1561 attaagaaga acaagtattt gccgagtgcc gaggatcttg cgtttccgac gacatttttt
     1621 gataataagg ttattcaggg cttgttgaaa aacaattatt ttcaatcgcg attaaagcgt
     1681 gagaaattaa ctgatttggt agatgaagag tatagtaaga tcatttatca gcaattaaaa
     1741 gaatacgcac cctataacaa ctatgttgga tcatcgcctg atttggttgc cgacaaaaag
     1801 atgctcaaag acatattcga acattttctt ttaaagaacg aatatttcga tgaacacatg
     1861 gaaaacagca ttgcgacctg ggcagatgat gaatttattg tgaagggatt gatggatgaa
     1921 ttcttcaata ataacatatc tgagcaaact gaaaaacacc tgtttgattt tcaggttggc
     1981 gatgaggaaa tggcatttgc agagtcgctg gtagtgttta ctatggagag cgatttgcaa
     2041 tatgatgagg aaatcaaacc taagcttcag aattgggaac tcgatcgtgt ttcattgatt
     2101 gaccggattc tgatgaaaat ggcattgaca gaatttttgc atttaccaac ggtgccgaca
     2161 aaagttacta ttaatgagta tctcgatatc agcaaattat atagcacacc aaggagtcgc
     2221 gagttcatca atggcatttt ggataagctg atgaatgaaa tgaaaagtag tggccgcatt
     2281 gtaaaaaccg gacgcggatt aatagaataa gcaatcatca accatatgaa aaaacaggta
     2341 ttatttgcaa gcttcgcttt aattttcggt gcaggcatat tattgtcctg caaggagcaa
     2401 tcgccggttg acgttaaggc caaatccggc agtgatgaaa aattaaatgc tgatttagta
     2461 aagaatcaga catcgatcac ctttgagcgc gacatgcatg actttggtga aattatcaag
     2521 ggtgagaaag tttcaacaga gtttaaattc atgaacacgg gtaaaaacga cttattgata
     2581 tccgatgcac atggcagttg cggatgcacg gtgcctgaat ggccgaaaga gcctatacct
     2641 cccggtggca gtggtgttat caaagttgtt ttcaatagcg gcaacagaag cggagaattc
     2701 aataaaacgg ttactgtaat tgcgaatacg gttacaaatc caaataagct tattatcaaa
     2761 ggaacaatta taacaccaac aaattaataa ttaaaagcaa ataaagatat gtcaatactc
     2821 aatgtacttc ttatggcgcc ggcagcagac gggcaatcat cgggctctag ctgggtgcag
     2881 atcgcatttt tctttctgtt cttcctgatt ctgtattttt tcatgatccg tccgcaatct
     2941 aaaaaagcaa aggagcaaaa gaacttttta aatgaaatca aagtaggtga taagattgtt
     3001 accataggtg gtgttcatgc aaagattctt aaaattgacg atgacaccta tttaatagag
     3061 gttgacacaa acaccaaatt gcgcattgaa aaatctgtga tctctttgga tttcacaaga
     3121 gcaatgttag cgcgtaagca aggttaatac tgcatggaaa ctatgggcga cagcaccccg
     3181 gctgcacaac gctcatttgc cggtgaattt gcattgtttt ttgtttgttt attcatagca
     3241 gtaattattt ggttatttac tgcgctcagt gaaaattatg aacaagaaat caatgttggt
     3301 ttatcctatg tattgcccga aagtcagact acaacaatac cattacctca agaagcccat
     3361 ttgctggtat cctcttcagg atggcaatta gtaaaagagg gatttcgtca tcgtgcgatg
     3421 tcattagatg tttcggatct tgatgacaaa ccggtattat tgacaaataa tcatcttgga
     3481 ttgtttacgg cggggcttcc ggggaatatg catattgtgc aggtgattcc tgatactata
     3541 gatttacatt tggagccctt ggcagagcga aaggttccga tagtattgaa tgctgatttc
     3601 atggatagaa cggatatcgt tgttgatagt atttatatac atcctgacag cattaccatt
     3661 cgtgggccga aaagtctgat tacggatatt acctactggt ctacagaggc aatagccggg
     3721 aatggtgatt caataataga tggcattaca cctctcccct ttcctgcggt tggcagcata
     3781 cgtttatcgg caatattggt tgattggcat gcagagatag aagcggcgat tgaggcaaat
     3841 gcgtatttga ctatcaatac acctactaat catgcagtag atgtacgtat tcaatatagc
     3901 agtgctgcag aagtttcgtc gttaataagt gctaaagatt ttaacattac cttcgaaaaa
     3961 actgctgatg gcacccaata tcatattgtg gcacgcgcta aaaggaaggg actgcatact
     4021 attggcgttt atccggcgtt tattccgata acaaggtgac atgataaagg ttggcattac
     4081 aggaggtata ggcagcggca aatcgacagt ttgcaaaata tttgaggtat taggtgtgcc
     4141 tgtttattat gccgacgagc gtgcaaagga gatacttcaa acggatgctg aaattattaa
     4201 gcgtgttaaa gatttatttg gtgaagacgt ttatgacagc aatcatgtac tcgacagaaa
     4261 gcgagtagca tttgtcgtat ttcacgaacc tgaattttta gcacagtaca ataaaataat
     4321 tcatcctgca gtattactag acagcgagaa atggatgcaa aggcatttgc aatatcctta
     4381 tgttataaaa gaagctgcgt tattatttga aagtggcgga aacaaacaac tagataaagt
     4441 gatttgtgtt acagcaccgg aagatttgcg cattcaacgt gtaatgcaac gcgataacac
     4501 cacagaagaa caagtgcgct cacgcatggc caatcagatg tcggaagaag agaaattaga
     4561 aaaaagtgat ttcatcctgt acaacgatgg ttcagcacca ttaattagac aagtattgaa
     4621 tattcatgaa aaattgctgg agatcatccg caagcaggat cagctggaaa agcgatgaag
     4681 gttaaaagcg aggagtaatg atagtatgtc gcattggtct tgacttatac attacgccta
     4741 tcacaggaac agcagtagcg gcgacatcat ttcctgtaag tccgagatga tagccgaaat
     4801 tcaaacccca gtctttatac attgtaaatc cggtttttaa tgccagtgcg taatattctt
     4861 gcgcattttg atacagcata gtagcagagg tagaacaatc gcaatcctca ccatctacgt
     4921 ttggtttgcg tgtatccagg ctaatggcac cccaccagta atcagcaaaa tgatatccgg
     4981 cttccaggtt tgaaactacc gcaagtgcat aatcatttgt tcttatagac ataccggtat
     5041 aaaactggag ccatgattta tcaaatcccc aaccgacgcc tatgccggga cgaaaagccc
     5101 agctattgaa tccggtttgc agtccggtga tataatttct gtcggaggta tttgcttcaa
     5161 caataaatga tgcggaaacg atgggtttat cgtcataaca tttccaaact agtccaaatt
     5221 ctgtattgcc gatgttattg agcttacctg tctcgatata ttgccccgga taactgttgt
     5281 atactccggg ataacctgtt gtttccaaca ttttatatgg caacctggct aaggcggtaa
     5341 ttctgtcggt gataccatat tgtacgtcca atgccaaagt tttatcaaat acggtcaagg
     5401 gcagatctat ggttgtagaa ttatcctgat aggccttatc atagtgaaac caggtataac
     5461 ttgctgaggc ctgaacacca tttttttcaa gtgtccaaat ctgggcctga caggaaagca
     5521 ctgtcagaaa caagaataat atagtggcag ataaacgcgt aactttgggt ttagaatttc
     5581 aaaaatacca ttttacttca aataacctag catgaccgct tcaaccttta cattgttgat
     5641 tgatatactc actttattgt tcatgatagt cggtgcattg tggatgtggg gtgtttttaa
     5701 attcaaagct gaggtacaac aacgatttga ggaaatcttt gcccgcagca ggaagcgatt
     5761 taaatggatg tttctaatag gtgcaatact attagcggca ttattcgtaa gggattattt
     5821 cctttaattc actggcaatt caaatggaag gtcgatgggg gtaagatcta ccatatggca
     5881 tttttcgcag aattgtggtg ttttgttttc ataccaaagc cttcggaatt cgcgatagcc
     5941 gggagtattg agacaataca tcaacccatc atcgaaaatg ttgccaaaat tcaactcctt
     6001 tggaaatggt tttgcgcaac aaggtgtagc atatccatcc catgaaatat agaagtgact
     6061 ccacgggaga tgacattttt ggtaggtatt aggtgttttt atatcccaaa tggtgagcgt
     6121 tacatttttc acttcgtcgg ccttatcctt tgctcgttta agttcatttt tgaaggcatc
     6181 ggaatgaaag aagtcgtaat aagaaatatc gtgagccgta actgctgcaa cattgaatgg
     6241 cgtaacattg atataatcaa cgccaagttc gcttgcgact tcaactactt cggccatgtt
     6301 gaaatagttt tctttgatgg caacgaaatt aaacatgaca tcagctctgc gatttttgca
     6361 tagttcaaca atacgtttaa cgttatcata aaacaaactg aactcgccac gcagccgcac
     6421 ttgttcatat acctctccta ctccatccat agagatttga agtgtatcca gatcatcggc
     6481 aattttttca accagctcaa cgctattggg caggtgcgca tttattgata tggtggtgat
     6541 gatgccttta ttttttgaac ggatatatgc caatgcttca ggcaactgct tataaagcag
     6601 tgtttcacct aagcctgtaa gcccgacaga atcgatatat ggataagcct gatcaattac
     6661 cttctttaat tgggcaatat ccataaatcc cttatccatt tcttcaccat agcggtactc
     6721 tcttggacag gtaatacatt tcaaattaca gtgattggta acctccagca tgatagacga
     6781 cggatgcgcc actttggtgc tgtggatcat gcgaaggtag gcgccatata ctttattgga
     6841 agtatagcgg aaaaaattgg ctcctcgcgg caaactttta agtctgcgaa ttgccttttt
     6901 tatcctcctg ctatttatgc caaacgccat ggcacaaatt tacaaataac tgttgagacc
     6961 gatatctata ctaaaccaca gatcagttca gccatgctga tcaatgacgg gaagggtgct
     7021 acatgaacat ttgaaatacc aatattttca agatatcgtg ctgttgttgg cccgatggct
     7081 ataaatgtct tgccatcagg agtatgctgt tttaaatacc cttcggcttg cattgggctt
     7141 gagcaggcga taatatcagc ttcaaagatt tcattgctga tcaaaatatt ttcataaacc
     7201 ggcaaggcaa aaacatgagc atagggggct attgcctcat gcagttcctt tcgggcattc
     7261 accgcacatg gaaataccac atgcattgtt tttccatgct catagaattg tttggcaaca
     7321 tcttctacta atccgtttcc ggtaaatgca acatcatatc caaatgttgt tattttttct
     7381 gctgtttctg caccaaaggc tgcaattttt atggatgatg gcatttgctt taccaccttg
     7441 aaaaattcat tcaccccatt tttgctggtg aaaaacagcc aatcgcaatc gatattaaaa
     7501 tcgaaatatt ttggcactat tttaatatat gattgcgcaa tcactgaatg tcctgccgct
     7561 tcaacaatct ttattgcctg ttctgctgca tccggttctg ttgtaaataa aacctttttg
     7621 tctgaccggt tgcgcagttt atcataaata acctcagcaa gattgtggtt gttcttgctg
     7681 cgcacaaata atcttcgagg cgtatttaat ttatcctcgg attgcgttgc ccaaacaaaa
     7741 aattcgccat gcatatattg gcaataaacg cccaagggca tatgacagcc gccattgaac
     7801 atttgcatta ctttgcgttc aacactgatg gtatcgtata cgctttggtg atgtaatttt
     7861 tcgagtattt ccaaaacttc gatatcctgt tctctgcact gatatgccaa cacaccttgt
     7921 gcgggagccg gaatgaaatg atgtggtgaa atttttatcg ccaataaatc atcgagtagc
     7981 aattgtaatc gctgtacacc tgctgccgca agcaatatag catcatactg tccgtttcgc
     8041 aatttctgca cacgagttgg cacattacct ctgatgtcag ccagttgaat atccggacga
     8101 tagaagtgca attgaacagc tcttcttgat gaagaagtgc cgacttttgc atgcagcggc
     8161 aaattccaaa attgtgtttt gtcggcaatt tccttgcgca ccaataataa ttccgaaggg
     8221 tcttcccggt aggaaacagc agctattttg agtcccggag tagttgctgt tggcaggtcc
     8281 ttatgagaat gaacggcaat atcaatttta ttttcaagaa gcgcgtcttc aatttccttg
     8341 gtaaaaaaac cttttccctc caatttctcg agactgacat gctgaatttg atcaccttgc
     8401 gttttgatga taaggatatc ggcatcaacg cctatttcaa gcaaagattt tttgagataa
     8461 tttgcctgcc ataatgcgag atcacttcct cgtgaaccga tgatcacttt atgtttcatc
     8521 agtgagggtg gcgcaaattg cttttagatg aagccatagt aagagccaga tactttttct
     8581 ccatataatc gagtaattta tccattactt cctttgcatg cggatcgagg gatgcaacat
     8641 cttttgcata cacttcttcc accgctctta accgaatttc atgtaatttt tgtggtattg
     8701 cgctgtgtgc tttttcaacc aggcgctcga tatatgcggc ttgaaattcc tttacaaact
     8761 catgcactaa ttttttagcc tttactactt ctgctttgcg caggctgagg tttttttgcg
     8821 caatgctgcg gagatcatcg atatggatgt aatctatttg attacgcgat aaaatagttg
     8881 tgtcgatatc ctgcgggata gcaagatcta taatcacttt cttttgtgta tcgcgaccaa
     8941 tcaattgttg atagatctcc tcattaatta caggctctac agagccggtg caactgatga
     9001 ttacatcaaa tccaccggta aatgttttca gggcatctaa ttgataagct ttaccaccga
     9061 ttgaagagac cagtttttct gcatttgcca atgtgcgatt aaacactgag aaattttgaa
     9121 aacccatatc agcaaggtag gccgacatat tagtaattgt ctcccctgcc ccaatcaaaa
     9181 taattgcggc atgttgaggt atttccaatt cccgcaaacg cagggcagca agtgaaacaa
     9241 cagaaacagg tttttcggca atatgtgttt ccgtatatat tttctttgca gcaggaatca
     9301 gcattttcat ggcaaggcga atatcatcac ctgtcagtgc catttcattc gactcttcgt
     9361 aagctacacg caattggcga ataatttcgc gttctccaac tacaagggaa tcaagtgagg
     9421 cactcacttc gcacatatgt tctattgctt cgacgccctc aaataactga gaatgcatca
     9481 cattacatgc ttcgaattcc gggtttactg ttttaaagaa gccggcagta aactgattgt
     9541 ctaccggttg agtagtagta aaaaaatatg ttactcgatt acaagtattg agatacatca
     9601 actcatccaa tttcatttcc tgcttaatgg attgcagttg ttgagaatag ttggttgttt
     9661 gcttatcgaa tggaactata taattgctga gttgccctag cgggacctgt ttatgtgtaa
     9721 cggttagtac tttatactgg ttcacggttt ctcctcttct tttcgctgct tgaaataact
     9781 atgcaaaggt cttatgacgc agccgtgaca ttgtcattga attgtcattt gccacctttt
     9841 acagggtgat tttactcacc gtttttgcac ttaatcgcgt gtgacagtga tgggaccggt
     9901 tgttaccgaa ttgctgagat ttcctgctct gtcgcgcatc cagacggtaa aagtgaccgt
     9961 ttcggaatcg ctgtcggggt ctacgaggat ggtattatgc aattgcacct ctaaatcgcc
    10021 ttctattgtt atttcggagc cttcaggagc cagccatggc acaaagaatg attcagtgag
    10081 cggaatgcgg ttgtcggtga tataaagcga catagaatca ggttcattat atcccaaatc
    10141 gccatcaccg tcaatataat ggatcagaaa tacgatggag tcctcgagtg ccaccactgt
    10201 gccgggagaa acggacatca attcaatttc ggggacttca gaaattggtg gatttatttc
    10261 ttcatctttc ttacatgccg acagaagcat gcaggcgata atgatataag ctgatgtccg
    10321 catggtcatt caattctgaa ggtgaaatag taggcgatat aaatttggga atcatcagga
    10381 agctcggtta caaaatcgtg agcggtatct ttcagatata atcgcatgaa atagagcttt
    10441 cctgtttcaa aatcatcggt atttaaggta aaatggtgcc agtatggggt tgggatcccg
    10501 gggtcgtaat atgcaggccc gtagtatgga tcgaaatcag gctccagttc aaaagtatat
    10561 tctgtaaatc cggtattcat gagcatgtcc tgagagatgc ggatattgta ttgttcgagc
    10621 catgatggca attgataaac tccactttcg tctgtatcat aaattccgaa ccaatattcc
    10681 acagtgctat gttgtgggag gataacaggt tgaaggaggt cagctcgatt tgtgtctaac
    10741 ctcaaccctg tggtgtcatt ttcatagcag atatagccgt atgttgaggg ttgataatca
    10801 ggtaatatgg gtgaattccc aaaaatatct ggcgcaccat ttaatatcca ttgcgtaatc
    10861 atttcacgtt catgcggata gagtgtatcg tataatggca ttcgtccgag cacggcatca
    10921 tcggtggtaa ttcgttcatg cagccatgaa agagcggtat caccgggtac aacacgataa
    10981 gtaaatgtat catcatcatc atttttaatt accggagcat agaccagtga attataggca
    11041 ctttgcaccg ttctaaaatc cggttcgaaa gtaccgtcgt ggcaacccgg cactgcgcag
    11101 gtggtgctga aaatgaaggt atgcaatccc aggaagctgg ctgaatcaat taaaaccggg
    11161 tcagtgcctc cgttgccata atcaacatca tcgtacggat tatcaggcag atccggttca
    11221 tcgcgaatga tctcacggca ggcgatcaat gtaaatgcac aaagcatcga catgacggcg
    11281 atcttggtta tcggaaattg tttcatacga atgcttgttc aagtactgtt ccgggtaaat
    11341 atcctgaagg tatattgtct ttaaatccga gaatatgcgc aatcgtcgga acaacatcta
    11401 ttgtttgccc gacaggttct tcttccgaac caacaacaag cccttgattt acgatagaag
    11461 gaggaccgac aacgaggcag aaaatatcgc gtgaattttc atcaccggta tgatcatagg
    11521 caaataatcc attggcatcc gggatggtgt ttgcgactgt atttctgcca tgttcaggca
    11581 cacaaatcat aacagtatca ttcgctaaaa tcggatcact ttgaatttta tcccataacc
    11641 atcctatgcc ataatccgcg cgatggagat aagatatata gctgctgaaa ttggtatggc
    11701 agatatcgag gttggtagta tttatcacca ttaattcagg ttgaaattgc tgcattacct
    11761 tccatgaagc ggcgatatta gtcagatcac cggtcatctg gtcgaaaccc acacctgcag
    11821 gtaaaggcca ttcaatagga atctcaccat gcagcaatcc gagcataaag tcttcaattg
    11881 cctgcttgtc ttcttcagaa tttctaatgc cgggtacatc aagtactgac ctgtcgaaat
    11941 tcgcatcaag gaaacttctt actgcattca ttctggttat ctcatcagga tgcatggtat
    12001 ttaaggtaga gaaataatct acaccaatat ttccgaaagt agtgatcggg ctcatgtaat
    12061 ttgcgccgta attcgcgccg tataatggat ccttgctata attaagataa acgtatggtc
    12121 ctaagtcaat tgacatccac catgcattga ttgcactttt cagagggtca ttgtgtttgc
    12181 gataatattc aaataatgtt ggcatggccg gatttgctga aagattgaga ctatcaggcg
    12241 tataattccc tgtgataaca gcggtatgcc cattaaaatg tccggtagga ccgttttcat
    12301 atcgcatatg tttaaacagg gtaccttttg tagcgagcgg agatgctaat gccggcgccc
    12361 attgatcata aaccagatta taggtaggtt gagcgcctga aagcatattc ggcatgatat
    12421 ttcctccggg gcccgctgcc tgattgagca gatattgctg actgacagat tcctgatttc
    12481 taattccacc gccaaataca actaacacta catgatttgc caatcgcata ccggaacttc
    12541 ttgacaaggt tccggcaggc aggatatatg gtgcagcaat catgcctgca gttgtcaata
    12601 aggagttgcg aatgaatttt ctacgattca tgatcagctg tatttttaat aaaacatata
    12661 ttcattacta agcaggaaag cacgatacac atcaactaca gtcatatcgg catccaattc
    12721 aatcatatcc gaaagcgcat attgttcata tgccgttggt ttgcgtaaat aaaaacgcag
    12781 atagcagtct tcaacaaact gatcaacatc accgcgcata tcagcatccg acgggatatt
    12841 tgccaataca gcagggtctt gcagcatcgc gtttaatatc aattcattta ctagagtttt
    12901 atcgccaatt gacaaacggt aaattacaag gttatcgagg atattgccgg ggatactcga
    12961 aaaatataaa ttactgtata atcctgaaat atattgcaat ggggttttta ctctatcctt
    13021 ttcttcattt gattgataaa cagggacagt atctactccg tagataatat tatcataaac
    13081 tacttcctga attacgtctt ttttacagga attagacaac agcactactc ctagtatgcc
    13141 taatgttgag aataatttag aagcctgcat attctgttga agttaagatg atcaatgcca
    13201 caccatttac attattttca agcatcattt gctgcgtgta agtatccatt tccagactat
    13261 tgggatccct tgcgaggtag gtattaaaag attcaattac caatccctgg aagaattcag
    13321 gtgtggaagt tacgatatca ataaaatcgc cctttgagct accatcctga agaaataata
    13381 catctgaaac accatcgacc atattgatcc cactttcttg ttcatcatca gtaggataac
    13441 gttgcataag atttgtaaag cagctgacca caaaattcag actacccata tttacctgat
    13501 cgtagaaata attattgata tatctacgat aaaattcatc ggcacctata gtcccatttg
    13561 caagatcgat atgtgcagtg attaatttaa taagtctatc gttttcaaaa atatagtatc
    13621 ctgcttcgcc ttccataccc atatcggtaa aaaggtcgat caggtaatcc cagtaagcta
    13681 tctgactggc aattgcaaaa ctgtcgacat ctaccagaaa tttttgtccg gtggtgaggt
    13741 ggatattatt gatatgctcc caatcggaaa gaagtgaatt taccagtcct tctcgagctt
    13801 caatactgta ttgttctgat tgaagcgtct ccgcatgttc acttagttca tctgcagtcg
    13861 ccgtcctacc aaagagatca atgtacaggc tgttgatata attatttagt gcaatttcag
    13921 acactcctgt aacaggtgga gcgatatttc catcgatgat caggttttca tggattacct
    13981 catccttagc gcaggaaaca gccagtaaat aaacagacga aataaggata agcccttgtc
    14041 gcatagtcgc cattttaagt gtgttagggt gtgtgcagac gttggggcga ctgtcactat
    14101 aacgtcatga aggtagtaaa tcgtatgtgt acacgcaaaa aaaaacgacc ccgatttcgc
    14161 ggggccgtct ccttttcata ctcgaactgt tttttccggg gctatggatt tatgtctttc
    14221 ttttcttgga atgcaataac gcaattgctt tgtgcgatat tgtatgagac aaaagtagat
    14281 cggtttggta cactgtacaa gagggagtat taacgaggtt gtacacattg tacaatatag
    14341 tacacggtgg tgttagagag tacgttttat taccttgaag gcaaggtaat cctttgatta
    14401 gaatttatat gcgatgccaa atccgatgct tcccagagag agcgaaatac tttgttgggt
    14461 aaataccgca tcagtagttt tatcctttgt ttgatagccg ctaaatccta gtgatccgca
    14521 tgctaattcc aagcgccagt ggtcattgaa aattatagaa gcccccggtc caataccgag
    14581 ggaataattt atcaagttat cggtttgctc attaatagat ccaaccccgt tatcgtcgaa
    14641 tactatggat tcatccgaat tctttccaaa gcctacataa gcgcctgctc caagccagag
    14701 gtcaacatac tgcgattgaa agggataata tcgggcaacg ggacctccac ctacactaga
    14761 gcgtgtaaaa tgcatatcca atgcaggatc atcaggatgg taaatagttg taatagaatt
    14821 gttgaatgct gctctcaacc ctatcatcca tttgtcgcca ataaaatatc caactgcagg
    14881 gcctgcatat attgttgacg atgatgggcc tttatcatca tatcctgttc cggatgagtt
    14941 tgaagtagtg tttgatagca acaagcttcc acccaataac caatcacctt cggtaaattg
    15001 agcttgagcg aatattggga tagatattgc cagaaaagct aagtggattt ttttcatcat
    15061 accaccaaat ataactattc aggaataatt tgggtggtat aagaaacacc gtatgcgcac
    15121 cagataccta atcccccaac aatattagtt tgtgcgatag tagcagaact aaatggacca
    15181 tcggtgccac tatcaaattc aagggttctc cagaaatcgt atgtagggaa atcgatatga
    15241 caccatttca gggtaacggt atcgcctttc caaaaatagc cataggttcc catgctgtaa
    15301 tcttctgtcg ggtcgtaacc tctgtcgaga gtaaaatcaa atgtttgacc attgatcagg
    15361 ttgtcatcaa aaacacttga gtatccggaa taaaaaggat cgctgtttct tctggtaaaa
    15421 taccgataaa aatcaccttg cggttccggt tcagaaagtg ttacatataa gcgcatcaag
    15481 gagtcgtttt ccggatcggg gtgcggttcg aaatacatcg tgtctaaagg gactgcaggc
    15541 aacaaacgcg ttgatgaagt aatagtttgt ccgttagcct cgatatgcag atcatatgtt
    15601 tttccttctt cacccacaaa tgatggtgaa cctgtaaaaa tatcagcgac cgtatacaaa
    15661 caaacgtcaa tagcaagttc accggctgta tccagctcaa cgccgagata ttcagcaaca
    15721 agaggtaaaa tctcatccgg taattcactt aaacaatatt caaccagttc aacagtatta
    15781 acaccatcac tcacggttac aacagcatca tgtacgtagt aattttccag ttcgttaaaa
    15841 tttacttcgc cataaaaagg tatcgttgaa gtcaatgaaa ttattggcgg cacaccattt
    15901 tcaatagagc cttctacaac tatttgcggc tcaggatcag gtatatcgag tgtaatatca
    15961 gttgtacatg aataaaaaga catcaataat atcccgaaag aaattacgcc gattattttt
    16021 gacttattgg ttcgaaacaa catgttagta aaaagtttaa gggatttata ttatttatgt
    16081 tgctgagcaa atttaaagtt ccatgttact gatggaatta tggggaaaat agacacctgt
    16141 ttcgcctgaa catccagata tccgtatgca aaatctcctt cgtaagtggt gtaaatgaaa
    16201 tacggatttt tacgcgaata cacattataa atactgaaat tccagctact ttcatatttc
    16261 ctttcaggtt tgctatgcaa tgtagcggat acatccagtc ggtgatatgc aggcagtcga
    16321 taaccatttc gcggaccata atcagtttgc acctgaccgc caattacgta aaaactttcc
    16381 actatggttg tagcctgtcc tgtggcataa acaaaggtgc ttgaaaagtc ccatctatcg
    16441 ctgagattgt aaacggcaac aacttcgaga tcatgtacgc gatcatattt ggttgggaac
    16501 cattccgagc ctgtgctgag gtcttcaaat ttacgttcgg ttttagagag tgtgtaacca
    16561 atccaaccat taaattttcc tttagctttt ttcaggaata tttctgcgcc ataagatcta
    16621 cctacgccaa acacgaaatt atcttctata tcaagtccca attcaggaac ataaccatct
    16681 ctgaattcga tttgattgcg caagtctttg tagtagactt ctatactcgt ttcataggca
    16741 ttatccttaa aattccggaa atagcctagg gcatattgaa taccaatttg aggttttacg
    16801 atgtcagagc ttggtatcca cagatcagta ggtaaagtac ttgttgaact gcttaccaaa
    16861 tgaatgtatt gattattgta tgtaatacct gctttcaacg acgactcggg atcgatggta
    16921 aagcgcatat taaaccgagg ctccagtccg gagtagtgtg ctatcatttc accgggagcg
    16981 taataaatgg tatcaataac tgttgctcca ttttcatcat aaactatatc attataaggg
    17041 cccacctgtg caaaatagct gcctcttaaa ccgatattta ttttcagtct atcagtaaca
    17101 tcaaattcat cttgaataaa tatgccggtt tcatgtgcat atttattact taacgtgtcg
    17161 gtagagaagt cgacatcatc aaaacttcct tgtgctgaat atggtgtaaa tgtatgatag
    17221 acataatttg ctccaaattt tatgtggtga tttgaaccgg ggtagtaatc gaaatccacc
    17281 ttcccattcc aatcctgaat gcctgaatag aggttgaaat taaaatcctg aaaatcgctt
    17341 tgcacggaga aattaaaact gttgtagata gcgctaacat tcataaacaa tttattattg
    17401 aacaggtggt tccatcgcac agtacctgtt gcatttcccc atggcacaga aacgtttaat
    17461 gacccatcgg gggcgctgta gttaaaaaca tcttggccga agtagccgct ggcaaaaatt
    17521 ctatttttat cgtcgaaggt atagttcact tttgtattca gatcgtagaa ataatagcca
    17581 ttcccttcaa aatccgttcc atttaaaaaa ggagttatca atgcatcgat atatgttcgg
    17641 cgaccgctaa caataaaaga gcttttatct tttacgattg gtccttgtat cgttaatcgt
    17701 gaagcaataa gtcctatgcc accctcagcc tggtagtatt gattattacc ttccttcatt
    17761 tgaacatcga gcacactgct gaggcgacca ccatattgtg aaggcatacc accttttacc
    17821 aatgtaacgt tttttatggc atcggaatta aacacgctaa agaaaccaaa taaatgtccg
    17881 gtattgtata ccacagcttc atccaataaa atcaggtttt gatcgggacc tccgccacgc
    17941 acataaaatc cggtattgcc ctctcctgcc ccaccaacgc ctggaaggag ttgaattgtt
    18001 ttaaggatat ctacttctcc aaacaacacg ggaattgttt tgatctgttc gatattcaat
    18061 tcaaccttcc ccatatcagt gctttggata ttcacatcac cgcgttctgc cttcacaact
    18121 acttcctcca ataatttacc ttgcgataat tgcacattta acctgacatc attatgcaat
    18181 gacacctttt ctatctgatt atcgtaacca acataactta ttactacatt gtaatcgccc
    18241 tccggtaggg tcatagagaa aaatccatat ccattagtag aagctcctgt gccggggtca
    18301 ttttgcagat acacgtttgc accaatcata tcctcaccgg taccttcatc cttcacatat
    18361 ccgctaagag tatattttcc ttgtgcaaac aacatgctgc atgcacacat aagaagcccg
    18421 aaaagtcctg ttttcatctg atactgccaa taacgccaca aagtaacgtt cagtttgctg
    18481 tacggcgtaa tttttttcta cgaacgcgga aaaaaggtcg acgaatgtca ttaaatctct
    18541 tgttcgatca ttgaatgtag atcaacttcg attcggagta gatgtcgccg gttagtcgaa
    18601 tggtatataa tccggtagcg agtctatcta agtcttcaat tctgattccg ctcatttttc
    18661 ccgcatcgat gagcaagtca gtgctcatga tcattttccc tgccatgtcg aacatatcga
    18721 atttagcctg atcagcttcg aatccttgga tataaacgac aagaacatcg ctgacaggat
    18781 taggataaac atggagtata tcatcattca aggtaatacc tttcagtgcc atataagcat
    18841 gcccgaattg aggaattcca tatcctatct ggttatttgg tgttccggca ttggtagcgg
    18901 tttgttgaat gatattgatg acatccatat ttgaaagatc gggaaatgcg gacatgaagc
    18961 tggcgcatgc ccctgcaatc aaaggactgg aataggatgt tccattgcca taaccgacaa
    19021 tgccttcagc cgacataacg gcagttccgg caccttgtcc aaccacgttc ggcttgatac
    19081 gaccatcaac cgttgggccg actgagctaa atccggcata atttccattc aaatcaactg
    19141 caccaatggt aaacacgcta tcaccatcag cgggggcgcc gataaaatgc cagggttcat
    19201 ctccactatt tcctgcacta ttgacaacta aaattcctgc tgcagcagcc atatctgctg
    19261 caattgttgc aacagtagtg ttgccatcca tatcgagata ggtatggtct tcggtagtgt
    19321 catcgaaagt agtgtaaccc aacgaggaat taatgacatc agcaccaatg atatctgcat
    19381 attctgctcc ttcgagccaa taatcttctt caacaacatg ctcactgtca gtcacctccg
    19441 tgcgaatgag gtaataatcg gcatcgggag cagagcctac atacacacct ggttcatcaa
    19501 cagccataat acttaacaca ttactgccgt gtgtagaact gaaatacaca aaatcggtat
    19561 cgtcgggaaa attataggtg ccttttatct gttctttggc gcgcacactt tcaaatccgg
    19621 cacctgtatt cacgccttca aagccaccat ctaaaacagc aatggtaata ccctgtccgc
    19681 ggaaacccag cgaatgcaaa aaatcgactt caatcatatg attttgattc tgcgctccgc
    19741 cataaacaaa atcagtgata tcggcggtgc gataaaatgc ctgatcttga ggtacgctaa
    19801 catcatctat aaggtgtcgt ttaataaccg gagtgatttg ctttacaaac gacaaagcgg
    19861 agatttccgc aatcatttca ggacctgtaa cagtgacact tactgcattg agccatctgc
    19921 tttctgtgcg tactaaagca ccggtttgtt taattgcgct aatatattgc ggatcgacgg
    19981 gaagatcaga ttttcgaatt ggaataagca ttcgctccct tctatcgata gcttgttcag
    20041 ataaaaatgc agatgggtta tcaatagtat aaggtgtggc atttttatcg ctaaaataca
    20101 cccatatatt ttgcgattga gcaaatgata cctcgtgcat cattatactg aaaacgaata
    20161 tgcctataat tcgaaaaaaa tacatgcaaa tctttattct ataaacacct tgacactttg
    20221 tttataccgc cccgacctga ccgtcagaaa ataaatacct gatgcataaa gatgcttatt
    20281 acccatttca acagttgttg gcatttctgc attgaggtac acattttcct gatgcatcag
    20341 tgcgccctgc aagttgtaaa cggcaaaagt agcttcacgg tttgttaatg attgcaatat
    20401 cacggtaaaa atatcaccat cgagcaatgg tattatggct atagatgata aagtggtgtc
    20461 aatggtggta tcaactgttg tatcaacaac ggtggtatca atttcgggga ataatgaatc
    20521 aagcaaagca tatgcttttc caaaattagg aatgccataa cccattgaat cgtttggtgt
    20581 atcgtacaag tgggcacttt gttccacagc atgcagtatt tgcatatttg tagcttcagg
    20641 gtatgcctgc cataaagttg cacaagcacc tgcgagtaat ggtgatgaaa atgatgtgcc
    20701 actgaggtaa ttgatatacc cattcggatc caacacagct gatccgagtg ccaatgctac
    20761 aacatttggc tttagtcgcc catcaaatgt aggaccatat ccactaaacc cgacatggat
    20821 accggaagtg tcaactccac caacactcag aacgctatcg ccatcagatg gcattgaaac
    20881 ataatgccat gcacctgctc cttcgttgcc ggcgctgttg caaaccaaaa ttccttttga
    20941 tgctgccata tcagcggcca ttgtaacaac agtggtattg ccatcgaggt cttcgtaagt
    21001 atgattttgt gttgggtcgt cgaatacggt atatcctaat gatgaattga taatatccgc
    21061 accaacacta tcagcacgtt cggcagcagc aagccagtga tcttcttcaa caacatattc
    21121 gctgtcggca atttctgtgc gatagaggta taagtcggca tttggtgcag atccgctgaa
    21181 aagatcagtc acttcaccgg cgatcacact catcacccag cttccatgat atcccgaata
    21241 aatattgaac acctgatcat tgttatccgg gaaattatga taccctaaga tttgatcttt
    21301 atcccaaaaa ctttgaaaca aggtagcagt atccacgcca aaaaaaccgg catcaagaac
    21361 ggcaatcacc attgaatcac ctgaaagtcc gatatcgaat aaggtatcga gattaatttg
    21421 ttcaatctga ttacttgcgg ctccgagtcc cccggtaatt tcagtattat ctgccttgcg
    21481 ataaaatgtt tgatcattaa gtggggtgcc ttgttcattg aggatttgat atctacctac
    21541 ccttgaaatt ttatttacga atggcaattc actcacctgc agcatttgat catcgctcat
    21601 gattacactt actgcattca accatttaga tcgcacatat acctggacac cgatattttc
    21661 gagtgattga atataatatt cacttacagg caggtcgctt tcagaaatag gaatatgttg
    21721 tttttcgcgg cgatttaaag atgcctttga tagaaattgc tccggtgatt gtttagaaaa
    21781 tgtattaaat tgtttatcgg taaagtatac ccaataacga tctgtatgtt gttgggcgaa
    21841 agaggtaatc gacaaacagc aaataaggca aataaggaga aatcgttgca tgaaaaatta
    21901 tagttgataa tcagttaatc gcatataaac actgaatccg cattgcacgc gttgttccca
    21961 tggacaatcc ggacaggtaa tatcttgcgt ataataatgt ttgaaagttt tttccaccat
    22021 accgacatcc aaggcataaa tctcttttgc ataatcaaac cagataaggt tagaatcgcc
    22081 gaattgttct acggtaattg tgttttcgaa atcgaaatcc ggaatggaat aattttcatt
    22141 tacatttgaa taaatataat cccatccttc ataataaatc aggcgattgc attcttcatc
    22201 ataaggcagg tcatctaatc ctccgagata tacatttcca tcccattgaa catcatttaa
    22261 tggaggagaa acaagtttta caaaacgcaa attattctca tttctgatca ttttgccatc
    22321 cacttgcatc acactccatg tattcgtcaa ttgccatgca tcgacatctg tttttctgcg
    22381 atatctgtca atgacataat taagatcgcc ggttaaatca taaaacgtat caatcaattc
    22441 ttctcggacg aaccagagag tggtgtcatt accggtagtt tcgtggtaga tgatagagtc
    22501 gacggcatac tcccaatagt ttcctatttt gagcgggcta tatgcgtatg gtgtttcagg
    22561 agtatttggt tcaggcaaag gtttatcaca acccattgag gcagcaaaaa tgaatgctgt
    22621 gagaatcaaa cccgttatgg atcgagaaaa cattaatcaa agatacaaga aagcttcaac
    22681 aggcggagaa tcaaggcatt tggtgcaatt ttcgaacaac cattagtaga ttcaattgta
    22741 ccaataaaaa gtaacaatca attacattta acggcggcaa tatttggcaa ggttgcatta
    22801 ctactgctga aaggcactca caatatgccc accaccaatc acatcgttgc cctcataaaa
    22861 cacggcgctt tggcccggtg taattgcgct cacattggag tcgaacgcta tgcgtagcag
    22921 atctcccggt tcgctgaaca gcgtactttc ggcacctgca tttttatatc gaattttcgt
    22981 caccgcacgc atcggctcgt tgatattttc atatttgagc atattgaggt gatgcacaaa
    23041 catgccattt ttattgagtt cttcttccag accaagcaca acaacatttt cgtgaggaat
    23101 gatctccgtc acaaaatatg gttcacctaa ggcaattccg aggccttttc gctgaccgat
    23161 agtataaaat ggatagcctt gatgagtgcc taacactttg ccgtgtttat cgacaaattt
    23221 tccgcctttt accttttcct ccaatccatc cacgcgacgt ttgaggaatc cgcggtaatc
    23281 attatcggga acaaaacaga tctcatagct ctcggccttg ttggcgagtt cggtgtaacc
    23341 catatctctt gccatttggc gtatttcagt cttatgaagt tgaccaagcg gaaatctggt
    23401 gcgacacagg caatcctgac tcaatcccca tagcacatag ctttggtcct tattttcatc
    23461 tttgccctta ctgataaagt agcgattgtt gtccggattt tggtgcacgc tggcgtagtg
    23521 accggtagca ataaattcac aacctaattg gtcggcacgt ttgagcagtg cttcccattt
    23581 gatatgggta ttacagagca cacaggggtt tggtgttctt ccagcgagat attcatctac
    23641 gaaattatcg attacatagt cgccaaattc ttcacgtata tcaactatga agtggtgaaa
    23701 ccctttgtcg acggcaacca tacgggcatc gttgattgaa tcgaggctgc agcaccctgt
    23761 ttcttttttt gagcctccgc ttgatgcata atcccatgtt ttcatggtaa taccgaccac
    23821 ctcataccct tgctcatgga gcatgatagc ggtaacgg
//
LOCUS       DAOQHY010000029        23612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_942_length_23612_cov_6.556565, whole genome shotgun sequence.
ACCESSION   DAOQHY010000029 DAOQHY010000000
VERSION     DAOQHY010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 23612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23612
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_942_length_23612_cov_6.556565"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PLL28_04010"
     CDS             <1..398
                     /locus_tag="PLL28_04010"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HNF68514.1"
                     /translation="ELFDRQQFIKYDADAPVTGADAGIMLCEALIARTGTILISSRQA
                     SGRTLPIFPEVNIVIASTKQLVLDIQDGFAAIRKHYDGNFPSMVNLNTGPSRTADIEK
                     TLVLGAHGPREVYVFLIDNWPMAGWDGKN"
     gene            385..1128
                     /locus_tag="PLL28_04015"
     CDS             385..1128
                     /locus_tag="PLL28_04015"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNF68515.1"
                     /translation="MERTKIYFASDFHLGIPNHSASLEREKKIVRWLDCIRHDAKQIF
                     LLGDTFDFWFEYDHVVPRGYIRLLGKLAELRDSGIDIQAFTGNHDLWMFGYFEEELNI
                     PVHHHPIFIESNGKKFYIGHGDGVGPGDRGFKLMKSVFTNRFAQFLYSLLHPYLAFKL
                     ARYFSHNSRMLNEEETFLGEEKEWQIIYAKEMLQKEHIDYFLFGHRHIVLELKLSDNS
                     TFINLGDWVRHYSFAEFDGQTCKIKYFEV"
     gene            1118..1903
                     /locus_tag="PLL28_04020"
     CDS             1118..1903
                     /locus_tag="PLL28_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68516.1"
                     /translation="MKFSILGIICMLQLQLGATDSLVCTIAVKPSYFTVDANGMLYYV
                     TASNEISRTDTCGNHTITYSNIANGTPTLIDVGNPLKILAFYKDQQKVVVLDKTMSEI
                     AVLSFNNINGKSYMPSLLCRATGGDHIWLFDEFTQSFVRLDESGNKIAASEPIYQIFE
                     KSEVPLWMVDIQDHLYVYTSGGLIYLFDAYGTPGTTFMLNDAPFSAYRNMFLIKGPTG
                     FSLVNIFTNEVIALPLPATGDVRLQNDLMYYYDGDHINVLQFR"
     gene            1978..3063
                     /gene="prfA"
                     /locus_tag="PLL28_04025"
     CDS             1978..3063
                     /gene="prfA"
                     /locus_tag="PLL28_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNF68517.1"
                     /translation="MHDQIDLTGKLQAIKERWLNIQEQLSDPDIIADMKRFKSLNKSY
                     RDMEPVVAAYERYQLVLSNLDDSRTILNTERDAELRDMAKEEIAAYETEKATLEEEIR
                     QLLIPKDPEDEKNAIIEIRAGTGGDEAALFARDLSEMYTRFCGKQGWRIEIMNENASD
                     QGGYKEIVLEVSGENVYGRLKYESGVHRVQRVPETESQGRVHTSAATVAVLPEAEEFD
                     VEINATDVKVDVFRASGAGGQHVNRTESAVRMTHIPTGIVAECQDERSQIKNREKAMK
                     VLRTRVYEKFHNEHLEKISKQRKTLVSTGDRSAKIRTYNFPQSRVTDHRINLTLYNLP
                     EVINGEIDEIINALQVAENAEKMKAEA"
     gene            3142..4443
                     /locus_tag="PLL28_04030"
     CDS             3142..4443
                     /locus_tag="PLL28_04030"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNF68518.1"
                     /translation="MKISVIGTGYVGLVTGTCFAETGNHVICVDIDAAKVQRMQQGEI
                     PIYEPGLETIFHRNIRQGNLQFTTDLKSAVEATDIIFLALPTPPNEDGSADLKYVLQV
                     AEDLGKIITSYKIIIDKSTVPVGTADMVRALIAKHCTTEFDVVSNPEFLREGMAVEDF
                     MKPERVVIGTESERAKEMLGRLYAPFVRQGNPVIFMDERSAELTKYAANAFLATKITF
                     MNEIANLCEILGADVDMVRKGIGSDSRIGKRFLFPGVGYGGSCFPKDVKALNQMAGVN
                     GYDFHILRSVMAVNEKQRVILADKVTSHFGNDLSGKHFAMWGLAFKPNTDDIREAPAL
                     YLIDALLERGATITAFDPEAMPNVEKQIGKRINYAVNQYEALSGADALIIVTEWSVFR
                     TPDIEAIKSALKSPVIFDGRNVFEVAEMKQAGIVYYSIGRV"
     gene            4584..4973
                     /locus_tag="PLL28_04035"
     CDS             4584..4973
                     /locus_tag="PLL28_04035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNF68519.1"
                     /translation="MGSFKSFEEMAVWLEARKVSQDIYLLTKKAEFSEDPKLRWQMRG
                     SSGSIMDNIAEGFSRGGTKEFIQFLRIAKGSSGELISQLHRCNDRNWISNNDFVTLCA
                     KTQKITGMLQSLIQYLQSTTRKGPTHQ"
     gene            5080..6066
                     /locus_tag="PLL28_04040"
     CDS             5080..6066
                     /locus_tag="PLL28_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNF68520.1"
                     /translation="MNSNHKPRVLITGAAGFLGSHLCDRFIAEGMHVMAMDNLITGDK
                     RNIEHLFELSNFEFYHHDVSQFVHVKGDLDYILHFASPASPIDYLKIPIQTLKVGSLG
                     THNLLGLAKAKQARILVASTSEVYGDPLVHPQSEDYWGNVNPVGPRGVYDEAKRFQEA
                     ITMAYHTYHGLETRIARIFNTYGPRMRLNDGRVLPAFIGQALRGEDLTVFGDGSQTRS
                     FCYVGDLIEGIYRLLMCDYAQPVNLGNPDEITINEFAEEIIKLTGTTQKIMHHPLPVD
                     DPKQRQPDISLAKKILGWEPKVHRSEGLQITYQYFQQLSKEDLYRDIEGRRF"
     gene            6223..7179
                     /locus_tag="PLL28_04045"
     CDS             6223..7179
                     /locus_tag="PLL28_04045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNF68521.1"
                     /translation="MSHLHIAKNVLLFESEQLRRTLDHLNDNFDAAVNAILQSKGKVV
                     VCGIGKSGAVAQKITATLCSTGTPAVFLHAAEAAHGDLGVYADGDPILMISKSGTTEE
                     MLRLIPFFLQSSSSVIAIVGNLNSPIARQSTIVLDGSVEKEADALNLAPTASTTVALA
                     LGDALAVALMHARGFTEKDFARFHPGGQLGKNLMLKVEDVMHEMPFIARVSEKNSFRE
                     LILAMTKFNLGAACVVNDAQELVGLVTDGDVRRSLLRDADLSSMSVSSIMTRNPVSVS
                     VHATLKEAVDLMENRSSQLSVLPVVEGKTCMGLIRIHDIYQG"
     gene            complement(7186..7707)
                     /locus_tag="PLL28_04050"
     CDS             complement(7186..7707)
                     /locus_tag="PLL28_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNF68522.1"
                     /translation="MLSLTPKSANIMYNVTVNGTQVYKVDGNTVNGNEILADVLKVDN
                     RFYHILTGGKSFNVEVVRLDKTAKTMTLQVNGTKYEVSLRDKFDALMEQMGFGSGAGQ
                     KAADLKAPMPGLVIEVRVQPGDQVKKGDPVIVLEAMKMENVLKAPGDGKVKSIEVQKG
                     VSVEKGLVLIKFE"
     gene            7754..10429
                     /locus_tag="PLL28_04055"
     CDS             7754..10429
                     /locus_tag="PLL28_04055"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNF68523.1"
                     /translation="MKLQPFIAVALAAALFYQCDPNKHIYKSEPYLGTIDTSGAAAYD
                     AYDYGYDSDAVGDYDYDYSYDYQNDTAYSPYSNAIYRGSSKREFDLLHTKLDVSFDWS
                     KAWMYGKATLTLKPYFHPMSTLTLDAKGFTVKEVSLVTATGRTPLKYVYNTDTIPDTL
                     QMTIDLGKTFKKDESFMIYIDYIAKPNDLPLGGSVAITGDKGLYFINNDGADPNKPMQ
                     IWTQGETEATSCWCPTIDKPNERCTTDFSITVADKYQTLSNGSLISSKKNTDGTRTDN
                     WKMDLPFAPYLFMMAVGEYAIVKDTWNKMQVNYYVEKDYEQDARAIFGNTPEMIDFYS
                     KKLGVNYPWPKYSQIVVRDYVSGAMENASATLHGEFLQQHKRELLDENYEDVISHELF
                     HHWFGDLVTCESWSNIPLNESFATYGEYLWREYKYGRDDADYIGYNDLQTYLNEASYK
                     MVDLIRYNYDTREDMFDSHSYAKGGRVLHMLRKYLGDDAFFAGLNLYLEKNKFQSVEI
                     HDLRKAMEEVSGEDLSWFFNEWFLDKGHAELSIYYEWFPEEDKLDITIYQNQDTSVAP
                     VYVIPMDVDFYYNGKVERKRITLDRSMQTFSFNFPVQPDLVNVDAEKMLLGNKYDNKD
                     IDSYIFQYQHAPLYRDRAESLEALAPYQAEDPRYRDLITSALDDKHWSLRQFAIDTLL
                     IDASTPQSVKEKIMQMAKSDPRSYVRSSALNKLKEITGIDAWSVYNAAMNDSSYNVVA
                     TAMTLIYNNDKKKGLELARTYKTEANDNLSYSIMGILSDGGEAIDNDYFLAKFKEASG
                     FEVYYVLMYYEPFILRMTDSYIIKKGVDELKKVASDQSSFWMSFTAVNTLSSMATTYQ
                     DAINNTTDLQMKQSLQSALDYVNAALTEVNGGGDY"
     gene            complement(10479..11720)
                     /locus_tag="PLL28_04060"
     CDS             complement(10479..11720)
                     /locus_tag="PLL28_04060"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNF68524.1"
                     /translation="MKFTLRIAWRYIFSRKSTNAINIISGISMSGIMIGSAALIIVLS
                     AFNGFENLVQRLYNTFYPDITVTPSYGKVFSPDSLMLQKIAHIDGVQDMSMTLEENAL
                     LAYNDQEHIATIKGVDTIYKKVTAVDDSVFYGQYVLEYEYLHTQIDCAVLGSGVASTL
                     NVALGLDNPSVQIFMPRRGTKGISNIQNTFIQRSIQPMGVFRIQQEFDSKYVITSLDF
                     IRNMLEYQNNEISAIEISVKGRAVDDVMDDIESIMGNEFTVKNRFMQNEFLYRVMRTE
                     KWAVYFILTFIFIIAAFNMIGSIAMLVIDKKKDIGILRSLGASEKSVRNIFFIQGILQ
                     TLVSIAVGFTLATLLCFLQMHFGLITIPGEGTFVVTAYPIALEWRDYVSVFLTVFIVG
                     CIASWFPAYMAARQRWLFKSE"
     gene            complement(11730..12092)
                     /gene="rbfA"
                     /locus_tag="PLL28_04065"
     CDS             complement(11730..12092)
                     /gene="rbfA"
                     /locus_tag="PLL28_04065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNF68525.1"
                     /translation="MDSRRQKKIGQLIQDEMGTFLQKEGANYYGRKFVTVTDAQVTPD
                     LQVCRIYVSVLDKNEGEKVVDSLNLHVRDIRKRFGQIMRQTLRIIPELEFYLDNTLEN
                     VFRLEEIFREQKKNEADQ"
     gene            12198..14015
                     /locus_tag="PLL28_04070"
     CDS             12198..14015
                     /locus_tag="PLL28_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF68526.1"
                     /translation="MKSYLRILKYVGRFKNYAILNFGFNLLYAFFNLFSILMVIPFLQ
                     IIFKMVEPPSQAPDFSWNIKQLMNYLNYYFAAYVSDHGAFAGLLIVCIAVVIMFLLKN
                     ICRYLALYYLAPLRTGIVRELRQGVYDKILQLPLSYFSEKRKGDIIARMSTDVKEVEN
                     SIMNTIESTFRDPVTMICFLIAMFVISFELTIFVLLLLPIAGLLIGRIGKSLKRKSQK
                     EQGQLGYLMSLFEESLSGLRIIKAFNAERYQKGKFLRENKQLEALATSAHRRWNVASP
                     LIEFLSVCVVAALLYYGGSIILRADSGLTADLFLGYILVFANMLGPARNVANAYFYIQ
                     RGIASLERIESIMNAEVQIKESLHATNIRTFDHAVEYQDVVFAYNETPVLRGINIKIE
                     KGKMIALVGPSGSGKSTFADLLARYHDVTSGSIRIDGNDIRDLRISSLRNQLGIVTQE
                     SILFNDTVFNNIAFGIEHASEEDVIKAAKVANAHDFIMRLENGYQTMIGDRGSKLSGG
                     ERQRITIARAIYKNPPILILDEATSSLDTENEKLVQDALFKLMKNRTSIVIAHRLSTI
                     QFADEIVVMQEGRIVERGNHNALIAKNGLYARLVEMQAF"
     gene            complement(14019..14786)
                     /locus_tag="PLL28_04075"
     CDS             complement(14019..14786)
                     /locus_tag="PLL28_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNF68527.1"
                     /translation="MDLSIFMHADAWIALLTLTILEIVLGIDNIIFISILSTKLPEAQ
                     QKPARRWGLMLAMGTRVLLLLSISWILGLEADLFNISGHGISGRDIVLLIGGFFLIYK
                     ATTEIHGKIEGEEHETSSKAKTPSFVSVVGQILLLDIVFSLDSVITAVGMVDADKVII
                     MIAAVIIAVIIMLFAADPISNFVQKHPTVKMLALSFLVMIGVSLIIEGWGGHVEKGYI
                     YTAMGFSVVVEMLNLRMKANAKKRHELRNMKENEFGN"
     gene            15008..15997
                     /locus_tag="PLL28_04080"
     CDS             15008..15997
                     /locus_tag="PLL28_04080"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNF68528.1"
                     /translation="MRQTPFFEIYLALFLLLGTLCFGVIGYMVLEGYNLVEALYMTVI
                     TLGTVGFTEVRPLDDAGRMFTIVLILAGLVIFAYFITQISRYFLDGEFILHYKSYKMK
                     KQIEGLKNHVIICGFGRNGREAARVLLKSNMHVVVIDQDESKLAEFAEEAHFYYIYGD
                     ATRDETLIDANITQASAILTTLPDDAQNVFVCLTAKELHQNIKIIARATHLSSVRKLK
                     NAGATNAIMPDKIGGAHMAMLVSNPDIEEFIDIMATHSQDDFSIQEVMAGKRVNLGDI
                     NCWQKTGATVIGIKSSGNQYILNPDANVSVNPGDGVIIMGSKIQINASKQLFI"
     gene            16001..16447
                     /locus_tag="PLL28_04085"
     CDS             16001..16447
                     /locus_tag="PLL28_04085"
                     /inference="COORDINATES: protein motif:HMM:NF019241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNF68529.1"
                     /translation="MTPQELLAIYRKNLESTIQEVTNFPDDTMLWETAPGVLNSAGNL
                     ALHIAGNLRYFLGNEIAGTGYVRDRDAEFNTKGLSRSEVCDALRLAIADVELTLANMP
                     KEMLGNNYPLDRFGGVQTTGFVLTYLLAHLNYHLGQVNYLRRILAK"
     gene            16465..17415
                     /locus_tag="PLL28_04090"
     CDS             16465..17415
                     /locus_tag="PLL28_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNF68530.1"
                     /translation="MQDVVLGIDIGGTGIKFGLVTRTGEILVTDKVKTKGYPNPEKLP
                     EDLLKWATKACSDHQVTLVGVGIGTPNGNYYKGTIDFAPNLPWKGVVTLKSFFESTFK
                     LPAALTNDAKAAAIGEMYFGGAKGMKDFLFITLGTGLGSGIVVNGQIAYGFDSLAGEV
                     GHTIVYRNGRECPCGRRGCLEQYVSAPGIVKTYYELLRSKNLDEFRTSIGNMIDSAEL
                     CKLAEDGDTLALEAFQKTGEILGFALSNSACYTRPEKIFLFGGLAQAGDLIFKPTIKS
                     FESYLLPIYQNKIPIVPSGLKESEAAILGSASLIWNELNK"
     gene            complement(17476..18735)
                     /locus_tag="PLL28_04095"
     CDS             complement(17476..18735)
                     /locus_tag="PLL28_04095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68531.1"
                     /translation="MLFKQATAILVCCASVLQISAQKDCSNDHLGRTPLIDLTDGYFN
                     GIRGGLYPEGSNIRPAEHLTECIEHVQAITPMDIYGNPNPNGKVVMLGIGASNPMTEF
                     QQFIDFSNDYEPVNDNLKIINACVGGQGIQKMFDITDNYWSGVVNGLEDQGLSAEQVQ
                     VVWIEQDNTRAYDTIFPSAPNALVNDYQLLLHVIHELFPNVQICYITARAYSGYADPV
                     DPEVSGGLLYPRDYFNGWAIKFLIEKVINHAFGFESEGPSAQIPLVTWGTYHWTDGST
                     PREDGLYLDCDLDVGPDGLHLSGVGEYKMGSLLFDYFKTDTTAKYWYLDEDFTGITTP
                     ETDVELRVWPNPVKNGTIHVDIPSTLIGADTQWMIYDMAGRAVLAGKEISGTELNIDV
                     TTLADGMYTFNCRDAQRQYHARMVIAH"
     gene            18918..21917
                     /locus_tag="PLL28_04100"
     CDS             18918..21917
                     /locus_tag="PLL28_04100"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HNF68532.1"
                     /translation="MKYRFITVAMLFSVGYAQVADQDLTKYVNPFIGTGGHGHTFPGA
                     TLPFGMVQLSPDTRPDGYNDWDGCGGYHYSDSIIYGFSHTHLSGTGVADYCDILLMPT
                     VGNIQLNNISGTDPLSGYASAFSHNTEHAEPGYYEVVLADDNIPVALTGTLRTGMHQY
                     SFPKTNQANVILDLRHRDKLLDGSYIEIVSPTKIQGLRRSSSWASDQWVYFAIEFSQP
                     FTVAGVVDSATTLKTAGTGFRPLILSGNQLHGYFRFNTDGAHPLMVKCALSPVSCAGA
                     WNNMATENPGWDFNAVRNNAKTAWNKELQKISIESPVDEARSLSDPDNKLVIFYTALY
                     HCMIAPNIYEDVDGKYRTRKNQIGITLNHTQYTVFSLWDTYRALHPLFTIIEPQRTND
                     FINTFLQQYQDGGDLPVWELSANETNCMIGYHAVSVIADAAVKGYSGFDKSLAFEAMV
                     HSANADRFGLKPYRLKGFIESTEEPESVSKTLEYAYDDWCIAQMSLLVSNKNINNDEY
                     NPTYDNFMNRGFGFIHLINENGFAQPRYNGGWYEPFDPFEVNFNYTEANSWQYSFAFP
                     QYLPFINTLEKKKQLEQQLDNLFSAKAKTSGREQADITGLIGQYAHGNEPSHHIAYLY
                     DYAGTPWKTQEKIHEICSKFYKNSPDGLIGNEDCGQMSAWYVMSALGIYQVCPGRPEY
                     ALTTPLFKKAVINNESPLIIQSNGDAEKNIYIHQVTLNGNNILVPIISHQQLMHGGTI
                     AYTISSATNIAWGTENMYTAALPKLPNKAPVLSPSKSTFENSTTISILCGANETIEYS
                     LNDGPLLTYTTPIKITENTVIKARAFNNTTNQSSPTVTGKYFLIPHNYNVTYQTAYNS
                     QYTGGGNTALIDGLRGNIDYRVGQWQGWWGDDMRVTIDLGKIQTIDTLSAEFLQDVRS
                     WIWMPQSLEVSISADGKHYTKLPTIQNTIPGDDYDSKITQVMQVAIPKELVRYIQVTA
                     TNHGPVPDWHPGKGGKSWIFCDEIWVK"
     gene            complement(21957..22811)
                     /locus_tag="PLL28_04105"
     CDS             complement(21957..22811)
                     /locus_tag="PLL28_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNF68533.1"
                     /translation="MSISLHMPLSIASLNSGSNANCYYVGNEDDAILVDAGLAYKETI
                     LRMEGLGLDPKRLKAVFISHEHTDHISGVTQLHRKLNLPVYITEKTRLNTALRFRDDL
                     IRPFIANAAIQIGSLHVLPFTKFHDAADPHSFMVYDNEVTIGILTDIGHACEEVINCF
                     KQCDAVFLESNYCEEMLANGSYPAQLKKRISGKSGHLSNRQALELFVKHKSERLQLVL
                     LAHLSQNNNTPEKALSEFRDHIGKIKLHVARRDGASELFEIHPIPAKVYNPPKPTAPK
                     PQQLSLFS"
     gene            complement(22914..>23612)
                     /locus_tag="PLL28_04110"
     CDS             complement(22914..>23612)
                     /locus_tag="PLL28_04110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68534.1"
                     /translation="VILQANTGFGCSYVWKKNGATIAGANTANYTATKSGNYTVMVTV
                     TGGCTATSATTTVTVNSKPNPVITALGDLNICALGSVTLKVTNKPGDTYQWYKNGVII
                     SGATANTYTATSIGSYHVKETTTAGCTKNATAVSVTSSCKLEAENITSLIVAPNPASD
                     VLYVMFNAGQTLQLFVSLTNTLGQQVYAKATTVESGEQTLTIELNNQLSDGVYYLSLQ
                     SENINFSKEIILAR"
BASE COUNT         7202 a         4749 c         4910 g         6751 t
ORIGIN      
        1 atgagctttt cgaccgtcag caattcataa aatacgatgc cgatgctccg gtaacaggtg
       61 ccgatgcggg gatcatgctt tgcgaagcac ttattgccag aacaggtact attttaatca
      121 gcagccgaca agcaagtgga cgcacactac cgatttttcc ggaagtgaat attgtgattg
      181 caagtaccaa acaacttgtg ttagatattc aggatggctt tgctgccata cgcaagcatt
      241 atgacggaaa cttcccaagt atggtgaatc tgaataccgg tccaagccgc actgccgata
      301 ttgaaaaaac acttgtgctt ggtgcacatg gtcccagaga agtatatgta tttctgattg
      361 ataactggcc aatggccgga tgggatggaa agaactaaga tatattttgc ttccgatttt
      421 catttaggca tccccaatca ttcagcaagt ctggaaaggg agaaaaagat cgtgcgctgg
      481 ctcgactgca ttcgccatga tgctaaacaa atatttcttt taggcgatac ttttgatttt
      541 tggtttgaat acgatcatgt tgttcctcgc ggttatattc gattgcttgg caagcttgca
      601 gaattgagag atagcggcat cgacatacag gcatttaccg gcaatcacga tttgtggatg
      661 tttggatatt ttgaagaaga actgaatatc cctgtgcacc atcatccgat tttcatagag
      721 tctaacggaa aaaaatttta catcggacac ggcgatggtg ttggccccgg tgatcgcggc
      781 tttaaattaa tgaaatcagt tttcacgaat cgctttgcac aatttttata cagtctgtta
      841 catccatact tagcattcaa acttgcgcgt tatttctcgc acaacagcag aatgttaaat
      901 gaagaggaaa catttcttgg cgaggaaaaa gaatggcaga taatttatgc aaaggagatg
      961 cttcaaaaag agcatatcga ctatttcctt tttggccatc ggcatatcgt tttagaatta
     1021 aaattgagtg acaacagtac atttattaac ttaggcgatt gggtgcgtca ttattctttt
     1081 gcagaatttg acggtcaaac ctgcaaaata aaatattttg aagtttagta ttttaggtat
     1141 catctgtatg ctgcaattgc agttgggtgc tactgattcg ctggtatgca ctattgccgt
     1201 aaaaccaagt tattttacgg tagatgcaaa tggcatgcta tattatgtca ctgcttcaaa
     1261 tgaaatcagc cgcaccgata cctgtggcaa ccataccatc acctacagca atattgccaa
     1321 tgggactcca actttgatag atgttggcaa cccgctgaag atactggcat tttacaaaga
     1381 tcagcaaaag gtggtagtgt tagataaaac catgtcggaa attgctgtgt tatcattcaa
     1441 caacatcaat ggtaaatcat atatgccctc attactttgc cgtgcaacag gaggtgatca
     1501 tatttggttg tttgatgaat ttacacaatc attcgtcaga ttggatgaga gtggaaacaa
     1561 aattgccgcc agcgaaccaa tatatcagat atttgaaaaa tcggaagtcc ccctttggat
     1621 ggttgacata caagaccatt tgtatgtgta tactagtggc ggattaatat atttgttcga
     1681 tgcatatggc actcccggta caacatttat gctcaacgat gcaccttttt cggcatatag
     1741 aaatatgttt ctcataaaag gaccaacagg attctctctt gtgaatatct tcaccaatga
     1801 agttattgcc ttaccactac ctgcaaccgg cgatgtcaga ttacaaaatg atttgatgta
     1861 ttattatgat ggcgatcata ttaatgtatt gcaattcagg tagccggatt aattgagtgg
     1921 atagcatcat taccattcat aaattattga caattaaata attaattttg catcatcatg
     1981 catgatcaaa tcgatctgac ggggaaatta caagcaatca aggagcgctg gctgaatatt
     2041 caggaacagc taagcgatcc ggatattatt gcagatatga agcgttttaa atcgctgaat
     2101 aaatcatatc gcgatatgga acctgttgtt gcagcgtatg agcgatatca actggtgttg
     2161 tcgaatttgg atgacagtcg cactatttta aatacagagc gcgatgcgga attgcgcgat
     2221 atggcgaagg aagaaattgc agcgtatgaa accgagaaag caacacttga agaagaaatc
     2281 agacaattat taatcccgaa agatcccgag gatgaaaaaa atgccatcat tgaaattcgc
     2341 gccggaacag gtggtgatga agcggcatta tttgcccgcg atctgagtga gatgtacaca
     2401 agattttgcg gcaaacaagg gtggcgaata gagatcatga atgaaaatgc gagtgatcag
     2461 ggtggatata aagaaattgt gttagaggta agtggcgaaa atgtttatgg cagattaaag
     2521 tatgaatcag gtgttcacag ggtacagcgt gttccggaaa cagaatcaca gggacgcgtg
     2581 cataccagtg cagctacagt tgctgtgcta cctgaggcag aagagtttga tgtagagatc
     2641 aatgctaccg acgtgaaagt agatgttttc agagcgagtg gcgccggtgg tcagcacgta
     2701 aacagaactg agagtgctgt gcgtatgaca catatcccaa cggggattgt tgccgagtgt
     2761 caggatgaac gctctcaaat taagaataga gaaaaagcca tgaaggtgtt gcgcacacgt
     2821 gtgtatgaaa aatttcataa cgagcacctt gagaaaatat cgaaacagcg taagaccttg
     2881 gtttctacag gcgaccgatc tgccaagatt cgcacctata attttcctca aagtcgcgtt
     2941 accgaccatc gcatcaattt aaccttatac aacctaccgg aagtgatcaa tggtgagata
     3001 gatgagatca tcaatgcgct tcaggtggct gaaaatgccg aaaaaatgaa ggcagaagcc
     3061 tgatacgaag caatttacct cggcatcttg cagcaatgta gtaaaatcgc tcaatacagg
     3121 ggttttcctt aattttgtgg tatgaaaatt tcagtgatcg gcaccggtta tgttggactg
     3181 gttacgggca cctgttttgc tgagacaggg aatcatgtga tttgcgttga tattgatgcg
     3241 gcaaaagtgc agcgtatgca acagggagag attccgattt acgagcccgg actggaaaca
     3301 atttttcaca gaaatatcag acaaggtaac ttacaattta ccaccgatct taaatctgcg
     3361 gttgaggcaa cagatattat ttttcttgca ttgccaaccc cgccaaatga agatggatct
     3421 gcagatctga aatatgtgtt gcaggtagcg gaagatttgg ggaaaattat cacttcgtat
     3481 aaaataatta ttgataaaag cacggttcct gttggcacag ccgatatggt aagggcattg
     3541 attgcgaaac attgcactac agaatttgat gttgtttcga atccggaatt tttgcgcgaa
     3601 ggaatggcgg tagaagattt catgaaacct gaacgcgtag tgataggcac agaaagcgaa
     3661 cgcgccaaag aaatgctcgg cagattatac gcaccatttg ttcgccaggg caatccggta
     3721 atttttatgg atgagcgcag cgccgagttg acaaaatatg cagccaatgc attccttgct
     3781 accaagatta ctttcatgaa tgagattgcg aatttgtgtg agatattagg tgccgatgta
     3841 gatatggtgc gcaaaggcat tggaagtgat agtcgcattg gcaaacgatt tttatttccc
     3901 ggtgtaggat atggtggaag ttgtttcccg aaagacgtga aagcattaaa tcaaatggcc
     3961 ggtgtgaatg gatatgattt tcatattctt cgttccgtta tggctgtaaa tgaaaaacaa
     4021 cgcgtgattt tagccgacaa ggttacatca cattttggca acgacctcag cggaaaacat
     4081 tttgccatgt ggggtctagc ctttaaacca aatactgacg acattcgcga agccccggca
     4141 ttatatttaa ttgatgcgct gcttgagcgg ggtgcaacca ttacagcttt tgatcccgaa
     4201 gctatgccga atgtagagaa gcaaattggc aaacgtatca attatgctgt taatcaatac
     4261 gaagcactct ccggagctga tgcacttata attgttaccg agtggagtgt gtttaggact
     4321 cctgatatcg aagcgataaa atcagcactt aaatctcctg tgatatttga tggtagaaat
     4381 gtttttgaag ttgctgaaat gaagcaagcg gggattgttt attatagtat tgggagggtt
     4441 tgaggttgga agttgagggt tgtaagttta aggttgtggg tttgagggtt gggagttggg
     4501 atttgatttg tgaggttatt tgtagttggt tgattattaa ttgaggggct gggtgattaa
     4561 tttcataatt aaattgttgc tgtttgggtt cgtttaagag ttttgaggag atggccgtgt
     4621 ggctggaggc tcggaaagta tctcaagata tttacctgtt gacaaaaaaa gctgaatttt
     4681 cagaagaccc aaaattgcga tggcaaatga gaggttcatc tggatctatc atggataata
     4741 ttgccgaagg attttcacgc ggtggcacaa aagaatttat ccaatttctg cgaattgcga
     4801 aaggaagttc aggagaatta atttctcaac ttcatcgttg caacgataga aattggatat
     4861 caaataatga ctttgtgacg ctctgcgcta aaactcaaaa aataactgga atgcttcaaa
     4921 gtcttattca atatctccaa tcaacaaccc gcaaaggccc aacccaccaa taacaactaa
     4981 ccaacaaccc tcaacttcca acctccaaca ttcaactttc aaccctcaac ttccaacatt
     5041 caactttcaa ctccatcata acctctatct ttaacatcaa tgaatagcaa ccacaaacct
     5101 cgtgtcctca tcaccggagc agccggattt ttaggctcgc atttatgtga tagatttatt
     5161 gcagaaggca tgcacgtgat ggcgatggat aatctcatca ccggcgacaa aagaaatatt
     5221 gaacatttat ttgaactttc caatttcgag ttctaccatc acgatgtatc gcagtttgtg
     5281 catgtaaagg gggatcttga ttacatatta catttcgcct caccggcatc gccgattgat
     5341 tatttaaaaa tcccgattca aacattaaaa gttggttcgc ttggaacgca taatttatta
     5401 ggactggcga aagcaaaaca agccagaata ttggtcgcct ctaccagtga agtttatggt
     5461 gatccactgg tgcatccgca gagtgaagat tattggggaa atgtgaatcc tgttggtccg
     5521 cgcggtgttt acgatgaagc gaagcgcttt caggaagcta ttaccatggc ctatcatacc
     5581 tatcatgggt tagaaacacg tatcgccaga atttttaata cctatggacc tcgtatgcga
     5641 ttaaatgatg gtcgcgtgct gccggcattc attggacaag cattgcgagg agaagacctt
     5701 actgttttcg gagatggcag ccaaacgcga tcattttgtt atgtaggtga tcttattgaa
     5761 gggatttatc gtcttttaat gtgtgattat gcgcaaccgg taaaccttgg caatcctgat
     5821 gaaattacca tcaatgagtt tgccgaagag atcattaaac tcacaggtac aacacaaaaa
     5881 atcatgcatc acccccttcc cgtcgacgat cctaagcaac gtcaacctga tatttcactt
     5941 gctaaaaaaa tactgggttg ggagcctaag gtacatcgca gcgaaggatt gcaaattacc
     6001 taccaatact ttcagcaatt atctaaagaa gatctatatc gcgatattga aggaagaagg
     6061 ttttaatttc ttgcacaatt gaatattctt aaatagtggc ccaaaattgc tttgtaaacc
     6121 atgacaatag tcactcaaat ctttgataaa cagcatgtca actatgaagc aaattcacgc
     6181 gttaattcgt aattttgtat aagtgctaaa tcagccaagt ttatgtcaca tttacacata
     6241 gccaaaaatg ttttgttgtt tgaatcggag cagttgcgcc gcacgctgga tcatttaaat
     6301 gacaattttg atgctgccgt aaatgccatt ttacaatcaa agggtaaagt tgttgtttgt
     6361 ggaataggaa aatccggagc agtggctcaa aagatcacgg caaccttgtg ttctaccggc
     6421 acacctgctg tatttctgca tgctgctgaa gcggcacatg gcgatctggg tgtatatgcc
     6481 gatggcgatc ctattctcat gatctctaaa agtggcacca ctgaagagat gcttcgatta
     6541 attccatttt tcctgcaatc atcctcatcg gttattgcta ttgtaggtaa tttaaattca
     6601 cctattgcgc gtcagtcgac catagtatta gatggttcag ttgaaaaaga agctgacgcc
     6661 ctcaatctgg cgccaaccgc atcaacaact gttgcacttg cattgggtga tgctttagct
     6721 gttgccttga tgcatgcaag gggatttacg gaaaaagatt tcgcgagatt tcatcccggt
     6781 gggcaattgg gaaaaaatct catgctcaag gttgaagatg tgatgcatga aatgcctttt
     6841 attgcacgtg tatcagaaaa aaattcattt cgtgaattaa ttcttgctat gacaaaattt
     6901 aatctgggcg ctgcatgcgt tgtaaatgat gcacaagaat tagttggcct tgttactgat
     6961 ggcgatgtaa gaagaagtct gctgcgcgat gccgatttaa gcagcatgtc ggtatccagt
     7021 atcatgacaa gaaatccggt aagtgtttct gtgcatgcta cattaaaaga agcagttgat
     7081 ttgatggaga atcgcagttc tcaattgtcg gtattgcctg tagtagaagg gaaaacctgt
     7141 atgggactta tccgcataca tgacatatat cagggatgat aatgatcatt caaatttaat
     7201 caacacaagt cctttttcaa cgcttacacc tttctgcact tcgatggatt taaccttacc
     7261 atcaccgggt gcttttagca cgttttccat tttcatggct tcgagcacaa tcaccgggtc
     7321 gccttttttt acctgatcgc cgggttgaac acgcacctca ataaccagtc ccggcattgg
     7381 tgcttttaga tccgcagctt tttgccccgc tccggaacca aaacccattt gttccatcaa
     7441 agcatcgaat ttatcgcgta acgatacttc gtatttggtg ccattcacct gcaaagtcat
     7501 ggttttagct gtcttatcaa gacgcactac ctccacatta aatgactttc ctcctgttaa
     7561 aatatggtaa aatcggttat ctacttttaa aacatcagcc agaatttcat ttccgttgac
     7621 agtattgcca tcaactttat aaacttgtgt tccgttcacg gttacgttgt acatgatatt
     7681 agcgcttttc ggcgttaaag atagcatgga atccgtaact ttggactcca ttcgatccaa
     7741 gtaatataca gatatgaaat tacaaccttt tatagccgtg gcactggcgg ctgctttatt
     7801 ctatcagtgc gatccaaaca aacatattta caaatccgaa ccctacctgg gaactatcga
     7861 tacatcagga gctgcagcgt atgatgccta tgattatggt tacgattccg atgcggttgg
     7921 tgattatgat tatgactata gttacgacta tcaaaatgat acggcatata gtccatatag
     7981 caatgccatc tatcgaggtt cgagcaaacg tgaattcgat ttgttacata caaaattaga
     8041 tgtatccttc gactggtcga aagcctggat gtatggcaaa gcaacactca cattaaagcc
     8101 atatttccat ccaatgtcaa cattaacctt ggatgccaaa ggatttaccg ttaaagaagt
     8161 ttcactggta accgcaaccg gaagaacacc gctgaaatat gtgtataata cagatacaat
     8221 tccagacacc ttacaaatga ccattgatct tggtaagaca ttcaaaaagg atgaaagctt
     8281 tatgatctat atagattata tcgccaaacc aaatgattta ccattgggag gtagtgttgc
     8341 cattacgggt gacaagggat tgtacttcat caataatgat ggagctgatc ctaataaacc
     8401 catgcaaatt tggactcaag gagaaacaga agccacttct tgctggtgcc cgaccatcga
     8461 taaaccaaac gaacgttgca ccacagattt ttccattaca gttgccgata aatatcaaac
     8521 actatcgaac ggatcgctga tttcttcgaa gaaaaataca gatggcacac gcaccgataa
     8581 ctggaaaatg gatttaccat tcgcaccata tttgttcatg atggccgttg gtgaatatgc
     8641 tattgttaaa gatacctgga acaaaatgca ggtaaactac tatgtagaaa aagattatga
     8701 acaagatgct cgcgccatat tcggcaatac acctgaaatg atcgacttct attcaaaaaa
     8761 attaggtgta aattatcctt ggccaaaata tagccagatt gtagtgcgtg attatgtgag
     8821 tggtgccatg gaaaatgcaa gcgcaacctt acatggcgaa ttccttcaac aacacaagcg
     8881 cgaactactt gacgaaaact atgaagatgt aatttctcat gaattatttc atcattggtt
     8941 tggcgattta gttacctgtg aaagctggag taatattcca ttgaatgaat ctttcgccac
     9001 atatggagaa tatctctggc gcgaatacaa atatggtcgc gatgatgctg attatatcgg
     9061 ctataatgac cttcaaacgt atttgaatga ggcatcatac aaaatggttg acctgattcg
     9121 ctataattat gatacacgcg aagacatgtt cgactctcac tcttatgcaa aaggtggaag
     9181 agtattacac atgctgcgta aatatcttgg cgatgatgct ttctttgcag gattaaattt
     9241 atatcttgaa aaaaataaat tccagtctgt tgaaatccac gatttgcgca aagcaatgga
     9301 agaagtgagt ggagaagacc tgagctggtt cttcaatgaa tggtttctcg acaagggtca
     9361 tgccgaatta agcatatatt atgaatggtt ccctgaagag gataaattag atatcaccat
     9421 ttatcaaaac caggacacat ccgttgcacc tgtctatgta ataccaatgg atgtagactt
     9481 ctattacaat ggaaaagttg agcgcaaacg catcacactt gatcgcagca tgcaaacatt
     9541 ttcattcaac tttcctgtgc aacccgattt ggtaaatgtc gatgctgaaa aaatgctact
     9601 tggaaataag tatgacaata aagatatcga cagttacata ttccagtatc aacatgcacc
     9661 attatatcgg gatagagccg aatcactcga agctttagca ccctaccagg cagaagatcc
     9721 gcgttaccgc gaccttataa catcggcgct tgatgataaa cattggtcgc ttcgtcaatt
     9781 tgccatagat acattgctta ttgatgccag cactccgcaa tctgtaaaag agaagatcat
     9841 gcaaatggct aaatctgatc caaggagtta cgtgcgttca tcagctttga ataaattaaa
     9901 agagatcacc ggtatagatg catggagcgt gtataatgca gctatgaacg acagctcata
     9961 caatgtagtt gctaccgcaa tgaccctaat ttataataac gataagaaaa aaggtcttga
    10021 attggctcgc acatataaaa ctgaagctaa tgataacttg agctatagta tcatgggcat
    10081 tctcagcgat gggggagaag caatcgacaa tgattatttc ctggcaaaat ttaaagaggc
    10141 cagtggcttt gaagtgtatt acgttttaat gtattatgag cctttcattt taagaatgac
    10201 agattcatac atcattaaaa agggtgtcga cgaattgaaa aaagttgctt ccgatcaaag
    10261 cagtttttgg atgtcgttta cagctgtgaa taccttaagc agcatggcaa ctacttatca
    10321 ggatgcgatc aataacacga ctgatttaca gatgaaacaa tcgttacaat cggcactgga
    10381 ttatgtaaat gccgctttaa cggaagtgaa tggtggtggc gattattgat aataccttat
    10441 cactatataa aagagcttcc aaccggaggc tctttttttt attcactttt gaacagccac
    10501 ctctgacgtg cagccatata agccggaaac caggaagcta tgcaaccaac aataaacacg
    10561 gtaagaaata cgctcacata atctcgccac tctaatgcaa tcggataagc cgtaacaaca
    10621 aacgttcctt cgccgggaat ggttattagt ccgaaatgca tttgtaaaaa acagagcagg
    10681 gtggccaaag taaatcccac agcaatgctc acaagtgttt gcaaaattcc ctgtatgaag
    10741 aaaatatttc ttaccgattt ttcagaagcg cccaacgatc tcaaaatacc aatatctttc
    10801 tttttatcga tcaccaacat ggctatggac ccaatcatat taaacgctgc gatgatgaaa
    10861 ataaaagtga ggataaaata aacagcccat ttctcggtgc gcattacacg atagagaaat
    10921 tcattctgca taaatctatt cttcacggta aattcgtttc ccataatgga ttcaatatca
    10981 tccattacat cgtcgacagc cctgcctttt acacttattt cgattgcact gatttcattg
    11041 ttttgatatt cgagcatgtt gcggataaag tcaagcgagg taatcacata tttactgtcg
    11101 aactcctgct gaatccgaaa cacacccata ggctgaatac tgcgctgtat aaaagtattc
    11161 tgaatattag aaataccctt agttcctctc cgaggcataa agatctgcac agaagggtta
    11221 tctaatccca aggctacatt taatgttgat gcaacaccgg agcccagcac agcacaatct
    11281 atttgcgtat gtaaatattc atattctaac acatactggc cataaaacac agaatcatct
    11341 acggcagtta ctttcttgta aatggtatca acacctttaa tagtagcaat atgttcctgg
    11401 tcattatatg ccaataatgc attttcttca agcgtcatgg acatatcttg cacaccatca
    11461 atatgtgcga ttttttgcag catcaatgag tcaggcgaaa acaccttccc ataacttggt
    11521 gtaacagtga tgtcaggata aaaagtattg tataatcgct gaaccaggtt ttcaaatcca
    11581 ttgaatgccg acaaaacaat aatgagtgcg gcactgccta tcataatccc cgacatggaa
    11641 atgccggaga tgatattaat ggcgtttgtc gattttcttg aaaaaatata ccgccaggct
    11701 atgcgaaggg taaacttcat gcaagagcgt tattgatcag cttcattttt cttctgctca
    11761 cggaatatct cttccagacg aaatacattt tcaagcgtat tatcgaggta aaattccaac
    11821 tccggtatga tacgcagtgt ttgtcgcatg atctgcccaa atcgcttgcg gatatcacgc
    11881 acatgcagat ttagtgaatc aacaaccttt tcgccttcat ttttatcgag aacgctcaca
    11941 tagatgcgac atacctgcaa gtcaggcgtc acttgagcat cggttacggt aacaaatttt
    12001 cgtccatagt aattggctcc ttctttctgc aaaaaggtgc ccatttcatc ctggatcaac
    12061 tgtccgatct tcttttgtct tctgctgtcc ataatctgct aagttacggt tttcggcaat
    12121 acaacgtcgt ttcgcgtctt ttagtttaga tgcaagggtg tatggaaccc ctcgtttttg
    12181 gaattttgtg acctatcatg aaaagttacc tgcggatatt gaagtacgtg gggcggttta
    12241 aaaactacgc catccttaat tttggattta acctgctcta tgccttcttc aacctgtttt
    12301 ctattttgat ggtgatacca tttctgcaga tcatcttcaa aatggtggaa cctccttccc
    12361 aagcgcccga cttctcatgg aacatcaagc aattgatgaa ttacctcaat tattatttcg
    12421 cagcttatgt atccgatcat ggggcgtttg ccggtctatt aattgtttgt attgcggtgg
    12481 tgattatgtt tcttctgaaa aacatctgcc gctatctcgc attatattat ctggctccat
    12541 tgcgaacagg aattgtgaga gaattacgcc aaggggtata tgacaaaatt ttacaattac
    12601 ccctctccta cttcagcgaa aaacgcaaag gcgacataat tgcgcgcatg agcaccgatg
    12661 taaaggaagt ggaaaacagc atcatgaata ccatcgaatc cacttttcgc gatccggtta
    12721 ccatgatatg ttttctaatt gccatgtttg tcatcagttt cgagctcaca atttttgtac
    12781 tattgttact gccgatagcg ggattgttaa ttggaaggat tggaaaatcg ctgaagcgta
    12841 aatcgcaaaa agaacaagga caattaggtt acttaatgtc cctgttcgag gaatcacttt
    12901 caggattgcg aatcattaaa gcctttaatg ccgaacgcta tcagaaagga aaattccttc
    12961 gcgagaataa acaactcgaa gcactggcaa ccagcgcaca tcgccgatgg aatgttgcaa
    13021 gtccgctgat tgagttttta agtgtttgcg ttgttgctgc cttactctat tatggcggaa
    13081 gtatcatttt gcgtgcagat tcaggactta ccgccgattt atttttagga tacattttag
    13141 tattcgccaa catgttaggt cctgcaagaa acgtggccaa tgcatatttc tacattcaac
    13201 gcggaattgc atcactggaa cgtatcgaaa gcattatgaa tgcagaagta caaattaaag
    13261 aaagtttaca cgctaccaat attcgcacat ttgatcatgc ggtagaatat caggatgtag
    13321 tgtttgcata taatgaaaca ccggtattac gcggtatcaa tattaaaatt gagaagggaa
    13381 aaatgattgc cttggtcggg ccttccggat caggtaaatc tacatttgca gatctgcttg
    13441 cccgctatca cgacgttact tccggatcta tacgcattga tggcaatgat attcgagatt
    13501 tacgcatttc aagtctgcgc aatcaattag gaatagtaac acaggagtcg atattgttca
    13561 acgataccgt gttcaataat atcgccttcg gtattgaaca tgcttctgaa gaagacgtaa
    13621 tcaaggcagc aaaagttgcc aatgcacacg atttcattat gcgtttggag aatggctatc
    13681 aaaccatgat cggcgacaga ggatctaagc tcagcggtgg agaacgtcag cgaattacca
    13741 ttgcaagagc gatatataag aatccgccca tccttatttt ggatgaagca acatcatctc
    13801 ttgatactga aaacgaaaaa cttgttcagg atgcattatt caaattaatg aagaaccgca
    13861 cctccatcgt aattgcacat cgactcagca ccattcaatt tgccgatgaa attgttgtaa
    13921 tgcaggaggg acgtattgta gaacgcggaa atcacaatgc attaattgcc aaaaacggct
    13981 tgtatgcaag gctcgttgaa atgcaggctt tttaattttc aattcccaaa ttcattctct
    14041 ttcatattgc gcagttcatg tcttttcttc gcatttgctt tcatgcggag attcagcatt
    14101 tcaactacca cagagaagcc cattgccgta tagatgtatc ctttttcaac atgaccgccc
    14161 catccttcaa taataaggct cacaccaatc atcaccaaaa atgacaaggc aagcattttt
    14221 actgttggat gtttttgcac aaaattgctg ataggatctg ctgcaaacaa cataataatc
    14281 accgcaataa ttacagcagc aatcattatg attactttat ctgcatcaac cataccaact
    14341 gccgtaataa cagagtcgag agaaaataca atatccagca aaagaatttg accaactacc
    14401 gatacaaatg aaggggtttt tgcctttgaa gaagtttcgt gttcctcacc ctcaattttg
    14461 ccatgaattt cggtcgtggc tttataaatg agaaaaaatc caccaatcaa taaaacaata
    14521 tctctacctg aaataccatg accgctgata ttaaataaat cagcttccaa tcctaaaatc
    14581 cagctgatcg acaacagtaa taacaccctg gttcccatgg ccagcattaa accccaacga
    14641 cgggcgggtt tttgttgcgc ttcaggtagc ttcgtagaaa gaattgatat gaaaatgata
    14701 ttgtcaatgc cgagtacaat ttcaagtatt gtaagcgtca ataaagcaat ccaggcgtcg
    14761 gcatgcatga atatcgataa gtccataggt tgggtttagc ggcaaaactg tgatgaaaag
    14821 ttcaaaatgc aaaaaaggaa atagttaata tgttcctgtt aataggtaat ttagacgctg
    14881 aaaagggcga aatatgctac tgaatcaatc gattttaccg taaaagccct caaaactcgc
    14941 gcttgaacgg ttttttcggt tttccttacg tgctatttca cctgtttacc ggaaatccag
    15001 caataccgtg cgtcagacac catttttcga aatttacctg gcattattcc tattacttgg
    15061 aaccttgtgt ttcggtgtaa tcggatatat ggttttagag ggatacaacc ttgtagaagc
    15121 cttatacatg accgtaatca cacttggaac tgttggcttt actgaagtgc ggccacttga
    15181 tgatgcaggc agaatgttta ccatcgtttt aattctggcc ggattagtca tttttgccta
    15241 tttcattaca cagatatccc gatattttct ggatggagaa tttattctcc actataaatc
    15301 ctataaaatg aaaaaacaaa tagaaggctt gaaaaaccat gtaatcattt gtggattcgg
    15361 tcgaaatgga agagaagctg cccgcgtatt gttgaaaagt aatatgcatg tggttgtgat
    15421 agaccaggac gagagcaaat tagccgaatt tgccgaggaa gctcattttt attacatcta
    15481 tggcgatgca accagggatg aaacgctgat tgatgccaat atcacgcaag ctagtgccat
    15541 cctgactact ttacccgatg atgcgcaaaa cgtatttgta tgccttacgg cgaaggagtt
    15601 acatcaaaac atcaagatca ttgcccgcgc aacgcacctg agttccgtaa gaaaattaaa
    15661 aaatgccggc gccacgaatg ccattatgcc cgataagata ggaggggcgc acatggccat
    15721 gcttgtaagc aatccggaca ttgaagaatt tattgatatc atggctactc actcgcagga
    15781 tgatttttct attcaggaag taatggcagg aaaacgcgtg aatctgggag atattaactg
    15841 ctggcaaaaa acaggggcta ccgttattgg aattaaatcg tcgggaaatc agtatatatt
    15901 aaatccggat gccaatgtaa gtgtaaatcc gggcgacggg gttatcatca tgggctctaa
    15961 aattcaaatt aacgcttcaa aacaactatt catttaagct atgacacctc aagaactact
    16021 ggccatctac cgcaaaaatc ttgaaagcac catccaggaa gtaaccaatt tcccagacga
    16081 caccatgcta tgggaaacag ccccgggagt gctcaactcg gcaggcaacc ttgcattgca
    16141 tattgccgga aacctccgat attttctggg caacgagatt gccggaacag gatatgtgcg
    16201 cgatcgcgat gccgaattca atacgaaagg cctttctcgt tcggaggttt gtgatgccct
    16261 gcgtctcgcc attgctgatg tagaacttac cctggcgaat atgcccaaag aaatgcttgg
    16321 caacaactat cctctcgatc gctttggtgg agtgcaaaca acaggatttg tgctgacata
    16381 tttactggca catttaaatt atcacctggg tcaggtgaat tacttgcgtc gcattttggc
    16441 gaaatgacct aattttatcg cgttatgcaa gacgtagttt tagggataga tatcggaggc
    16501 accggtatta aatttggttt ggtaacaaga accggtgaaa tactcgttac agataaagta
    16561 aaaaccaaag gatatccgaa ccccgaaaag ctacccgaag accttttgaa atgggctacc
    16621 aaagcctgct ccgatcatca agtaactctt gtcggtgttg gcataggcac gccaaatggc
    16681 aattattata aaggcaccat tgacttcgcc cctaacctgc catggaaggg cgtggtaacc
    16741 ttaaaatctt ttttcgaatc caccttcaaa ttacccgcag ccctcaccaa cgatgccaaa
    16801 gcagcagcta taggtgaaat gtattttggc ggtgcgaagg ggatgaaaga ttttttattt
    16861 atcaccctcg gaacaggttt gggtagcggc attgtggtaa atggacaaat tgcctatgga
    16921 ttcgacagcc ttgccggaga agttggtcat accatcgtat atcgcaatgg tcgcgaatgc
    16981 ccttgtggaa gaaggggttg tctagaacaa tatgtaagcg ctccgggaat tgtaaaaaca
    17041 tattacgaac tcctgcgaag caaaaatctt gatgaattca gaaccagcat cggcaacatg
    17101 atagatagcg ccgaattatg caagcttgca gaagatggcg acaccttagc attggaagca
    17161 tttcaaaaaa ccggagaaat tcttggcttt gctttgtcaa attcggcatg ctatacgcga
    17221 ccggaaaaaa tatttctttt cggtggctta gcccaagctg gtgatttaat tttcaaacct
    17281 accatcaaaa gttttgagtc atatttattg ccgatttacc aaaataaaat tccaattgta
    17341 ccttccggat taaaggaaag tgaggccgcc attttaggaa gcgcatcatt gatttggaat
    17401 gaattaaata aataaaaaat gccaccattt gcaggcggca ttctcttaac atatatataa
    17461 acgggtcacg aataattaat gagcgattac catgcgtgca tgatattgcc tttgtgcatc
    17521 cctgcaattg aatgtataca ttccatcggc caaagttgtt acatcaatat tcaattcagt
    17581 tccactaatt tcttttccgg ccaatactgc tcttccagcc atgtcataaa tcatccattg
    17641 cgtatcagca ccaatcagtg ttgatggtat atctacatga attgtgccat ttttaacagg
    17701 attaggccaa acgcgcaatt caacatcggt ttccggtgtc gtaataccgg taaaatcttc
    17761 atccagatac cagtatttag cagtggtatc tgtcttgaaa taatcaaaaa gcaacgatcc
    17821 cattttatac tctccaacac ctgaaagatg caatccgtca ggccctacat ccagatcgca
    17881 atcaagatac aacccgtctt cacgaggagt agatccatcc gtccaatgat atgttcccca
    17941 agtgactaaa gggatttgcg cagaggggcc ctccgactca aaaccgaatg catgattgat
    18001 aaccttttcg attaaaaatt ttatagccca accattaaaa taatctcttg gatataacaa
    18061 tccgcccgaa acttcagggt ctactggatc agcataacca ctataagcac gtgctgtgat
    18121 atagcatatt tgtacattgg gaaataattc atgtattaca tgtaataaca attgataatc
    18181 atttaccaat gcatttggtg ccgaaggaaa aatggtatca taggcacggg tattatcttg
    18241 ttctatccaa actacctgca cctgctctgc acttaaaccc tgatcctcaa gtccattcac
    18301 cacgccactc caataattat ctgtgatgtc gaacattttt tgaatgcctt gtccaccgac
    18361 gcaagcattg ataattttta aattgtcgtt taccggttca taatcatttg aaaagtcaat
    18421 aaactgttgg aattctgtca ttgggtttga agcgccaatg ccgagcatta caactttgcc
    18481 atttggattt ggatttccat aaatatccat tggtgtaatc gcttgcacat gctcaataca
    18541 ttctgttaaa tgttcagcag gacggatatt acttccctcc ggatataatc cgcctctgat
    18601 tccattaaag tacccgtcgg tgagatcaat taaaggagtt ctgccgagat gatcattcga
    18661 acaatctttt tgggcagaga tttgtaagac acttgcgcaa cacaccaata ttgctgtcgc
    18721 ttgtttaaat aacataggct tttggttgat gataatatag acatgcggtg tgttgtcatc
    18781 atcaatatta caaataacgt gctgaaactc aaaacactag tgccacaaag ggatgttaaa
    18841 tttttgtata acccatgtgg ggaatctttc cccaagctgt gcaaggcatc tacatcatta
    18901 ttctttatct tcatcgcatg aaataccggt tcattaccgt ggcaatgctc ttttctgttg
    18961 ggtatgccca agttgctgat caagacctta cgaaatatgt gaatccattt ataggtaccg
    19021 ggggccatgg gcatactttt ccgggagcaa ctttgccctt tggcatggtg cagttgagtc
    19081 ctgacacccg ccccgacggt tataacgatt gggatggctg tggcggatat cattacagcg
    19141 attctattat ttatggattt tcacatacac atctcagcgg taccggtgtt gccgattatt
    19201 gtgatatcct gctgatgcct acagtaggaa atattcagtt gaataatatt tctggcacag
    19261 atccgctttc gggatatgcc tccgcatttt cgcacaacac agaacatgct gaaccgggat
    19321 attatgaggt ggtgcttgcc gatgataata ttcctgttgc ccttaccgga accttgcgaa
    19381 caggtatgca tcaatatagc ttccctaaaa ccaatcaggc taatgttatt ctcgatcttc
    19441 gacatcgcga taaattgctc gatggctcct atatagaaat tgtatcacca acaaaaattc
    19501 aaggtctccg tcgatcatca tcatgggcaa gtgatcaatg ggtttatttt gccattgagt
    19561 tttctcaacc atttactgtt gccggagtgg ttgattccgc cactacgcta aaaaccgcag
    19621 gcacaggttt cagaccacta atcctgagtg gcaatcaact tcatggatat tttcgcttta
    19681 ataccgatgg cgcacatcca ttgatggtga aatgcgcact ctctcctgtt agttgtgccg
    19741 gagcgtggaa taatatggca acagaaaatc cgggttggga tttcaatgct gtacgcaaca
    19801 atgctaaaac cgcatggaat aaagaattac aaaaaatcag catagaaagt cctgttgatg
    19861 aggcaagatc actttcagat cctgataata agctggttat tttttatacg gcactgtatc
    19921 actgcatgat agcacctaat atttacgaag atgttgatgg caaatatcga acaaggaaaa
    19981 atcaaattgg cattacacta aatcacacac agtatactgt attttcatta tgggatactt
    20041 acagggcatt gcatccatta tttacgataa ttgaaccgca acgcaccaat gatttcatca
    20101 atactttttt acaacagtat caggatggtg gtgatcttcc ggtttgggaa ttgagtgcaa
    20161 atgaaacaaa ttgtatgata ggatatcatg ccgtaagtgt gattgccgat gctgcagtta
    20221 aagggtattc gggatttgat aaatctcttg ctttcgaggc gatggtccat tctgcaaatg
    20281 ccgatagatt tggccttaag ccctatcgct tgaaaggttt tattgaaagc accgaggagc
    20341 cggaatcagt atctaaaacg cttgaatatg cttacgacga ttggtgtatt gctcaaatgt
    20401 cattgcttgt ttcaaataaa aatatcaata atgatgaata taacccaacc tacgacaatt
    20461 tcatgaacag aggatttggt tttattcatc ttatcaatga aaatggcttc gcgcaacctc
    20521 gatacaatgg cggatggtat gaaccatttg atccttttga agtgaatttc aattatacgg
    20581 aagccaatag ctggcaatat tcatttgcgt ttcctcaata tttaccattt attaatacac
    20641 ttgaaaaaaa gaaacaatta gaacaacagc tagataattt atttagtgcg aaagccaaaa
    20701 cctccggacg cgagcaggct gatattacag gacttattgg tcagtatgca catggcaatg
    20761 aacccagtca ccacattgca tatttatatg attatgccgg cacgccgtgg aagactcaag
    20821 agaagattca cgagatatgc agcaaatttt acaaaaattc acctgatgga ttaatcggga
    20881 atgaagactg tggtcagatg agtgcctggt atgtaatgag tgccttaggc atttaccagg
    20941 tttgccccgg aaggccggaa tatgcactca ctactccttt atttaaaaag gccgtgataa
    21001 ataacgaaag tccacttatt atacaatcga acggtgatgc agaaaaaaat atttacatcc
    21061 atcaggtgac attgaatggc aataatatct tagtgccaat aatatcacat caacaactca
    21121 tgcatggtgg cacaatcgca tacaccatat catcagctac caatattgca tggggaacag
    21181 aaaacatgta caccgctgca ttgccaaagc ttccgaataa agctcctgta ctatcaccat
    21241 caaaaagtac ttttgaaaac agcactacca tttcaatctt gtgcggtgct aatgaaacaa
    21301 tagaatacag tttaaatgat gggcctttac tgacatatac aacgccaata aagattacag
    21361 aaaatacagt gataaaagcg cgtgcattta ataatactac aaatcaatca agccctacgg
    21421 taacaggaaa atattttttg attccacata attacaacgt gacatatcaa actgcatata
    21481 acagtcaata tacagggggt ggaaatacag ccctcataga tggcttacgg ggaaatatcg
    21541 attaccgtgt aggacagtgg caaggttggt ggggtgatga tatgcgtgtc accatcgatt
    21601 tgggaaagat ccaaacaata gatacattga gtgccgaatt tctgcaggat gtgcgcagct
    21661 ggatttggat gccccaatca ttggaagtaa gcatctctgc tgatggcaaa cactacacca
    21721 aattgcccac tatacaaaac acgattccgg gagatgatta cgatagcaaa ataacgcagg
    21781 ttatgcaggt tgccataccg aaagaattag tacggtatat acaagttact gcaacaaatc
    21841 acggtcccgt tcctgattgg catccgggga aaggaggaaa gagttggata ttctgcgatg
    21901 aaatttgggt gaagtaattt caatgcacac catcttgtaa taattcacca gttgcattaa
    21961 gaaaacaaac tcaattgctg tggcttcggc gctgtgggtt ttggcggatt atacacttta
    22021 gccggtatag gatgaatctc aaacaactca gaagcaccat cgcgtcgggc aacgtgtaat
    22081 ttaatttttc cgatatggtc tcgaaattca gataacgcct tttccggtgt attgttgttt
    22141 tgagaaaggt gtgcaagcaa aactaattgc aatcgctcgg atttatgttt tacaaataat
    22201 tccaatgctt gcctgttgct taagtgtccg gacttcccgc taattctctt tttcaattga
    22261 gccggataag atccgttagc aagcatttct tcacaataat tcgactcgag aaaaacagca
    22321 tcgcattgct tgaagcaatt aattacctcc tcgcaagcat ggccaatatc tgttagtatg
    22381 ccaatggtga cttcgttgtc atacaccata aaactatgtg gatctgccgc atcatgaaat
    22441 ttggtgaagg gaagtacatg cagcgagccg atttgaatag ccgcattagc tatgaatgga
    22501 cgaatcagat catctctaaa acggagggca gtattcaggc gtgttttttc tgttatataa
    22561 acagggagat tcagcttgcg gtgtaattgc gttacgccac tgatatgatc cgtatgttca
    22621 tgtgaaataa atactgcttt caaacgcttt ggatcaagtc cgagaccttc cattcgaagg
    22681 attgtctctt tgtaggctaa tcccgcatca accaaaatag catcatcttc attccccaca
    22741 taatagcaat tggcattaga gcctgaatta agtgatgcta tggaaagagg catatgtaaa
    22801 gatatactca aaaaataaaa ttgagtgaac caaaactgtt aaagcaacaa aaaaccgcaa
    22861 tgttgaaaaa aacaacactg cggcttacaa atcattttag cgatagtatc aactcatcgc
    22921 gccaggatga tttcctttga aaaatttatg ttttctgatt gcagcgacag gtaatataca
    22981 ccgtcggaca attgattatt aagttcgatg gtcagcgttt gttctcccga ctcgaccgta
    23041 gttgccttag cataaacttg ttgccctaag gtgttggtta atgaaacgaa caattgtaat
    23101 gtttgcccgg cattaaacat cacatataat acatcgcttg caggattagg tgcaacgatc
    23161 agcgaagtaa tattttcagc ttcgagttta catgaagagg taacactaac tgccgttgca
    23221 tttttcgtac aaccggcggt agtagtttct ttcacgtggt atgaaccgat agatgttgca
    23281 gtataggtat tagcggttgc accagaaatg attacaccat ttttatacca ttggtaagta
    23341 tcgccgggtt tattggttac tttaagtgtg actgaaccga gtgcgcatat attcaaatca
    23401 cccagcgctg taatgacagg atttggtttg ctgttaacgg taacggttgt tgtagctgaa
    23461 gtagcggtgc agccaccggt aacggtaacc atcactgtat aatttccgga tttggtggca
    23521 gtatagtttg cagtattagc acctgcaata gtggcgccat ttttcttcca cacgtatgaa
    23581 catccaaagc cggtatttgc ttgcaaaatc ac
//
LOCUS       DAOQHY010000030        23598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_943_length_23598_cov_5.588795, whole genome shotgun sequence.
ACCESSION   DAOQHY010000030 DAOQHY010000000
VERSION     DAOQHY010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 23598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23598
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_943_length_23598_cov_5.588795"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PLL28_04115"
     CDS             complement(<1..359)
                     /locus_tag="PLL28_04115"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNF68535.1"
                     /translation="MKKIAVMTSGGDSPGMNACIRAVVRTGLYHGLEVYGIRRGYEGM
                     IDGDFFKMESYHVSNIIQRGGTILKTARSERFMTAEGRKMAFDNLKLNGIEGCVAIGG
                     DGTFRGATVFEKEFGIS"
     gene            complement(450..1388)
                     /locus_tag="PLL28_04120"
     CDS             complement(450..1388)
                     /locus_tag="PLL28_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNF68536.1"
                     /translation="MPGETILIIGACGQIGAELTMFLRHQYGGSNIIAADIKAPGGEL
                     LESGPFEEINVLDQAGIAGIIDKYKVTQIYNMAAMLSATGERFPHKAWELNMEGLINT
                     LELAREKNIRRMYWPSSIAAFGPTTPKVDTPQITIMEPNTIYGISKQAGERWCEWYHQ
                     KFGLDVRSLRYPGIISYKTQPGGGTTDYAVDIYFKAKAQGAYTCFLAHDMMLPMMYMP
                     DAIRATVELMEASADKISVRSSYNLAAMSFNPAMVGESIRKYMPDFKLDYEIDFRQKI
                     AETWPGSIDDHQARQDWGWKHAYDLDAMTKDMLDHV"
     gene            1491..2582
                     /gene="wecB"
                     /locus_tag="PLL28_04125"
     CDS             1491..2582
                     /gene="wecB"
                     /locus_tag="PLL28_04125"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNF68537.1"
                     /translation="MKKILVVVGTRPNFIKVALFKSMAAMHGFDLRIVHTGQHYDAAM
                     ADVFFRQLALQPDYFLELPSLQPEQQVILIQAKLNRLMGSTFMPDLVMVVGDVNSTRA
                     AAEAAHILNIPIAHVESGLRSFDQSMPEEINRIVADNLASLFFITEQSGIDNLREEGK
                     PENKMHLVGNTMIDTLVHFAPLIDQQNILEQWQLEEKRYYLVTLHRPSNVDEKNGLQQ
                     AIECLNWLSEDLPVVFPIHPRTKKQFLQFGLDENISSNKNIIQLPPLDYFGFQKLIKS
                     ATAVITDSGGIQEETTFYGVPCLTMRPNTERPVTINLGSNELVENNIDSLRPFIQQIR
                     TGHYKNISIPPLWDGRATERIFQILAALR"
     gene            complement(2558..3871)
                     /locus_tag="PLL28_04130"
     CDS             complement(2558..3871)
                     /locus_tag="PLL28_04130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF68538.1"
                     /translation="MKRVLIITYYWPPAGGVAVQRWVKMARYLRQFGWEPVIYTAENG
                     EMPVIDESLAATLPAGLEVIKRPIWEPYSWYKRFLGLSKEEKINAAFLQEKKKTGLLQ
                     HLAIWVRGNLFIPDARRFWIKPSVRFLTDYVKSHPVDAIISTGPPHSMHLIAMGLKER
                     TGIPWIADFRDPWTRIDYYHELHLTSFADKRHHQLEKQVATNADALVTVSMQWANDFN
                     LLNNGNSTVITNGYDEDDFPAVHPDPEKGFVLHHVGMINKARNPETLWLAISNLCKTL
                     PGLSDDLRIVFTGKNDYSVHEFLEKYGLTGYAENRGQVTHAEAINAMCSATILLLLIN
                     DAQDILGRIPAKLFEYMAAKRPILAIGDPKGDAATILEETGAGILIDLKDVDGLQSTI
                     ASWYADYKSGKLSVKSGGIEKYARKSVTEQYARLLDSLHRKAARI"
     gene            3974..6472
                     /locus_tag="PLL28_04135"
     CDS             3974..6472
                     /locus_tag="PLL28_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68539.1"
                     /translation="MQKNNGSKKLLTYLAVFVFFLAFSALYFYPLLQGKILFQSDIMN
                     FVGVSKEAVDHYDTTGDAALWTNSMFGGMPTYQVSLPENGNIFERINIYLGAFLPRPA
                     NYMFIALVTFFLLMRSFRVNTWLSISGALCYALGTYLITFMEAGHNTKVNALALMPLV
                     FTGLQYLWQRKWLLGSALTLFAMTCEITANHPQINYYMFLIIAIWGISELVFAIREKT
                     VLQFVKITAVIIAVTLMAVAANTSKLWTTYEYSKESIRGGSQLTSQKTVQDDGLNRDY
                     AFGWSSGVSESFSIMYPNFAGGGSGFSFLQTRDGETIDGPMLPFIQQLYQKDPKKTQE
                     ILNYSGQAGKYWGDMPFTSGPIYVGSILCFLFILGALLADNRMRWWLIAATVLSLFLG
                     WGKNFEAFNYWMFDHFPLYNKFRTVSMAMTMLCFTIPVLAIYIVNKFLSDNNEITHEK
                     KYWALKWASLATGALSLILMMSGSLFDLLKPVEEEYLMQANSIEASTFFEMLKDARAQ
                     MIRTDVLTTTFIIALAAGALWLYLRKTITAKIAIAIICILPVLDLMIVDSKYLGKDNY
                     QEANFYDQRLRSSLPVIKDKDPYYRVWNTMADPDKDGTTSYLYKSIGGYHGAKLQRYQ
                     DIIDGYLHKGDVHVLSMLNTKYVIGQRQGKITFDRNPAALGNVWFVDSVRMVKTADEE
                     FNGLANFSPQNTVIVNEEFKNLIPATTIQRDSTASIKLKAYGMDEISYTSKSSVEMPA
                     VFSEIWYRGNQDWKAYIDGKYVDHFRANYLLRGLWVPAGEHEIVFKFEPASFAAGKKV
                     STASSGLILLLVAIGFFLAGRKAIQENKQTEEQA"
     gene            6474..7283
                     /locus_tag="PLL28_04140"
     CDS             6474..7283
                     /locus_tag="PLL28_04140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF68540.1"
                     /translation="MQYDPVKRSLGKVFNTTPFLRIVFYKMLDLLLLRAWHIHKALRQ
                     WSKGKKETEQHILDAGSGFGQYSYFLSGMSNKWKFLSVDVKEEQIGDCNNFFQKIGRK
                     NIRFQVKDLTTFTIPDHFDLVVCVDVMEHVLEDVQVFKNYHTSLKTGGMVLISTPSDQ
                     GGSDVHEETGSSFIEEHVRDGYNIQEIQDKLTSAGFTRTEAHYAYGTPGKISWRLSMK
                     YPLQLLNISKLFFIILPVYYIITFPFCLLLNACDVAMSHKTGTGLIVKAWK"
     gene            complement(7289..8566)
                     /locus_tag="PLL28_04145"
     CDS             complement(7289..8566)
                     /locus_tag="PLL28_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68541.1"
                     /translation="MRTITCRIESPLVSEYTWVMKVFAKYIGFNVQFVDGNEDMLIAE
                     HGMGDIQVSHFFRMTYQSGDCHFKSYFRKELLHYTASNKPDYLSTCFYLLSYLQEYTD
                     YHPDQYGRFPFSNSIQHHFDCIDKNLVAQYFTLLYEQTPKLKQLVSKKEHASVVFLSH
                     DIDSAYGALAENGKWLLKHGKVGALMQLIFNHYVRTPDHLLLDKIMQIEDAYDVKSVF
                     FWLARSGRGTRLINNADYNLSDSKVRSIQQHISEKGWVNGLHKSAGKSTMTADYNSVR
                     EFAEPVNRNHYLLTELPGTFNEIEAAGIQMDCTMGFAEAPGFRNSYGLPVRPYHFKEK
                     REYNFIEVPLHIMDTTYRYYRKESGKEAERLIIDFLEHNPQDAVISILWHNNYFFDRT
                     DTSWLDAYKAVLQYMHEKQIKSVLPKELLEEYL"
     gene            complement(8636..10189)
                     /locus_tag="PLL28_04150"
     CDS             complement(8636..10189)
                     /locus_tag="PLL28_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNF68542.1"
                     /translation="MDTMIQGNAIAAELKALGILSNNSGVSTGVNWLKGEGNGVESFS
                     PVDGSLISSCTSASREEYEQIMQTAAAAFKEWRMWPAPKRGEVVRQLGEKLREHKQDL
                     GKLVSYEMGKSLQEGLGEVQEMIDICDFAVGLSRQLYGLSMHSERPLHRMYEQWHPLG
                     IVGVISAFNFPVAVWSWNAALAFVCGDVCIWKPSSKVPLSAIATQKIIAEVFKANNVP
                     EGVSCLMVSHSSIGEEMAKDARVPLISVTGSTRVGKRIGGIVGERLGRVIQELGGNNA
                     IIISEHANMNIAIRAAIFGAVGTAGQRCTTTRRLIIHESVYADVLNLLKKSYAQLRIG
                     NPLDQNNHVGPLIDRKAVEDYEKAIAQAMEEGGKMIVEGGVCSGEGYESGCYVKPCII
                     EASNNFKIVQHETFAPILYVMKYSTIEEALDMQNGVPQGLSSSIMSTNIYETEYFLSQ
                     QGSDCGIANVNIGTSGAEIGGAFGGEKETGGGRESGSDAWKAYMRRQTNTINYSTQLP
                     LAQGIKFDI"
     assembly_gap    10369..10468
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10469..11482
                     /locus_tag="PLL28_04155"
     CDS             <10469..11482
                     /locus_tag="PLL28_04155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68543.1"
                     /translation="RLVRIYILLLTLTGVVSRTEAQVNVPGNGAIWTYTIDDVDLTHG
                     TCFYRFKYVLYADIDSTINGYNYTLLKLKQTLYYTFDPFNQFYCSIDTFDHDLGIFGG
                     IRSLDEKTYFYGLNNPLQIILGLDVPDTTDVLVYDFSKIPGDTLPRKDYYGYIMYDSI
                     KETSYVTLADGIIRKQQTVVYKSYTVGISGDTILANSDQNIYVEGIGDIENGLFFPIS
                     PQYSSYYVSLNCYKENEIYLLGDNSCENEDAQFETPTLEGKTIIAYPNPFKEYFRYEV
                     PYDHSTIRLYTLDGKLVLEQVTDLPNGYISTRHIPNGAYIVTCTASDGKVAYDIICKA
                     ATY"
     gene            11554..12165
                     /locus_tag="PLL28_04160"
     CDS             11554..12165
                     /locus_tag="PLL28_04160"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNF68544.1"
                     /translation="MGTRINAVDQYILKSQAFAQPILNHLRDLVHTVCPDVEEKIKWG
                     MPFFDYKNDMLCHMASFKAHAVFSFWKAQLMKDPILMENANSEQAMGHLGRITSLKDL
                     PSDKQLRAWIKEAMVLNDKGIKIVKPKSTGEKPTVEIPEMLIKALKKNKSAWAVWQKA
                     TPGYQKEYANWISDAKTEATQIKRTNTAVEWIAEGKIRNWKYQ"
     gene            complement(12421..15471)
                     /locus_tag="PLL28_04165"
     CDS             complement(12421..15471)
                     /locus_tag="PLL28_04165"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNF68545.1"
                     /translation="MKKLSLILSLLLTLKLQQSVYAQQASYWQQHVDYEINVVLDDVN
                     HMLNGSIAMRYTNNSPDVLNEIWIHLWPNAYKDNSTAFAKQKLESGDVKFHFADESER
                     GFIDNLNFKVNGEEVTLTYDEETPDIAKITLNKPLSPGQTINIKTPFRVKVPNCFSRM
                     GHDEQQYLITQWYPKPAVYDRKGWHAIPYLDQGEFYSEFGNFHVFITVPKNYVVGASG
                     DLLTASEVTFLDSIAAVTATKTFETKDLSFPPSSPTTKTLEYMLKDAHDFAWFADKRF
                     NVMKSSVTLPESERVVTTYAYFNDEHGQEWLKAAEYVDRAVAFYSDNVGEYPWNVAQA
                     VDGSLEVEGAGGMEYPTITVITGKFDAKMLDNVITHEVGHNWFYGILGSNEREHGWMD
                     EGMNTFYENRYMATYYEPANQLGIPNFALAALNVDTSDINETLWHINQTTQKQNRYQP
                     VDLHSKYYTSANYGLVMYMQTGYDFRYLEDVLGTDKYDQIMRKYYRQYQFKHVYPEDM
                     QAVFESETGDSLGWFFQQLLQSDRGPDYSIKHLLKSKTAMAVTVKNNSDIPAAFTIAL
                     MSGDSIIRTDWFRGFTGSQTVYLNYNENMHVTALRIDARKKMPETERENNTIKTSGLF
                     KRVEPLQVRLFGFGADNPNRTTISVMPLVGWNDHDRWMLGLGIWNSTMPTPKIEYVLA
                     PMYSIYSGEPVGQGSVGLNFYPDNGLFDRIRISESAARYTYDEFYINALFDQAQYKPQ
                     YLKLESKIEADLHPKSMRSKLTQRLSARSIFIQEDDLYFLYDINGDITGTASASRMEY
                     ELGYHLAYDRTFFPQKVDALLTAGEGFTKLSATYHTTIHYPGMKKGMNVRLFGGAFLQ
                     DGGDENQSFTLAGNNGYYDATYDDVFLGRNESSGFLSTQMTATDGFFKVPVYNYAFTS
                     RSYMGAANFELTIPKVPLVSLFADAGYFGLRESVTGISGFQYDAGLKIGLPLDIFSLY
                     LPFVMSSDLADQFADDASYGEKMSFQLSINALNVFEMMRKLAVE"
     gene            15604..17022
                     /locus_tag="PLL28_04170"
     CDS             15604..17022
                     /locus_tag="PLL28_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF68546.1"
                     /translation="MMDHDFNEQDPTAIAELIEQFERYLKDNEYAYFDEESLELILEY
                     YEMRNLNERAEAVIAFALEQNPFSSDFLVRKAEYLLNRKRYQEAMDTLDKAMLFDSRE
                     IDIYLLRSDIYAEQNKLTEAEAILLEALEIADEEDKDAVYAELSDIYEIQEQFDKAFD
                     CLAKALSLNPSSEEALYKLAHIVDMTDRYDDSVSIHTGIIDKDPYAWLAWYNLGRAYI
                     GLGLYEKAMEAFEYVMAIDEHFDLVYRDAADLYYRMEAFEKAISMFEIAHEKSGGYED
                     YSFRIGLCHERLNNFKSARFQYRKATRQDPFMHEAFFRIGETYRLEERLEPALVNYKK
                     ALQVDDTNEDYICTIISIYKMLDREDDVLEHLRILVNVRADILSYWLDFLIYLFDIGQ
                     FEKTIEIADEAIRRSGHFAEYYYIQAAALSALGDGKSALVLLEHALTIDYGRHTILYE
                     LNREIALLPAFQQVILQFAPRS"
     gene            17082..17840
                     /locus_tag="PLL28_04175"
     CDS             17082..17840
                     /locus_tag="PLL28_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HNF68547.1"
                     /translation="MNFNLTRLPERTSKPRKEGLCMVMDKGLSVREAEDLLSVSEPYI
                     DLIKLGFGTAFVTPNIENKIAVYKNAGIPTYFGGTLFEAFLVRGQFEDYLRLLEKYKM
                     SYAEVSDGSIELNHDEKCGYINRLSKHVTVLSEVGSKDENKMIAPYKWIELMKKEIEA
                     GSYKVIAEARESGTVGIYHSKGEVRSDLIDEILTQVPQDQIIWEAPLKGQQVYFIKLL
                     GANVNLGNIPPNEVIPLETLRLGLRGDTFHHFLT"
     gene            17851..18507
                     /locus_tag="PLL28_04180"
     CDS             17851..18507
                     /locus_tag="PLL28_04180"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNF68548.1"
                     /translation="MEFLHTFIDTFLHLDQYLESWIDTYQNWIYVILFAIIFIETGIV
                     IWPWLPGDSLLFAAGAFCAIGSLNIFIIIPLLFVAAVLGDTSNYFIGKYFGERAMRVK
                     WRGKPILKPEHLAKTHAFYEKYGAKTIVLARFVPIVRTIAPFVAGMGTMTYKTFFSYN
                     IIGAILWIPSITVLGYIFGQNEFVKHNFEIVVLAIIFISVLPIFYELFKARFKRSKNN
                     "
     gene            18519..19277
                     /locus_tag="PLL28_04185"
     CDS             18519..19277
                     /locus_tag="PLL28_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNF68549.1"
                     /translation="MKHFGLIGFPLSHSFSETYFREKFSREEIEDCIYELFPLENIED
                     VRLLFEVNKDLVGLNVTIPYKESVIEYLDDLDPVAQKIGAVNCIHIDEIQRTGYNTDW
                     MGFRDAIKPFLNKPHQHALVLGTGGASKAVVYALRELGLHVTLVSRSSGNKKIAYGDI
                     TSDIIAHHTVIVNCTPLGMYPNTNECPDIPYDLITDQHLLFDLIYNPGETLFLQKGKA
                     KGATISNGLEMLRLQAEYAWEIWNQQSEESEAEM"
     gene            complement(19348..20265)
                     /locus_tag="PLL28_04190"
     CDS             complement(19348..20265)
                     /locus_tag="PLL28_04190"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HNF68550.1"
                     /translation="MLKKEFIKFSTSGLVMLLLTTHLYAQPITLSREIGANLGFSSFL
                     GDLGGSDDVGRAFWWDIDPQITRPALGFVYRQEIIPRTAFRFNAYASILKGDDALTDN
                     EFRHYRNLSFRSPIVEASGSVELSAFRFTGVKKKKWTPYIYGGVGAFYFNPQAQYNGE
                     WVNLQPLGTEGQGLPEYPDRQKYSKIAVCFPVGGGFRYITDRDWVLGFEMAARFTNTD
                     YIDDVSGYYPNPDYYYLHYDLETALMAATLSDPSDHTIPEWTQPEYGRGDPTNNDSYI
                     FGGMLTFTYHMEFQRKVNTIKCYFNQDNK"
     gene            complement(20377..21501)
                     /gene="rffA"
                     /locus_tag="PLL28_04195"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(20377..21501)
                     /gene="rffA"
                     /locus_tag="PLL28_04195"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR02379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HNF68551.1"
                     /translation="MIPFNKPSTLGSEHTHMLAAVETGKLSGNGLYTHQCHHYFEQHF
                     GVHRALLTHSCTGALEMSALLCNIKPGDEIIMPTYTFVSTANAFALRGAVIKFVDSKP
                     DHPVMQVEDIEPLVNAKTKAIVVVHYGGYSVDMQPVLDLAIRKGLFVIEDAAQAIGSR
                     YHGVLCGTIGHFGAFSFHETKNVHCGEGGLLAVNDERYADRAEVIWEKGTNRSAFFRG
                     EASKYEWMDLGSSFLPSELQAAWLWGQLEHYSGIRSQRAVLWQAYREPLMEMQKAGYF
                     ELLEEQAGSTHNHHLVAVICKDSIERGKLIQFLEKQGVMAVSHYLCLHKSPYYSEKHD
                     GREMPNALRFERNLLRLPLFHDLSLSGVEKITEVLKSFYA"
     gene            complement(21504..22238)
                     /locus_tag="PLL28_04200"
     CDS             complement(21504..22238)
                     /locus_tag="PLL28_04200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNF68552.1"
                     /translation="MEQTPEISVISPVYNSEGNVPELVQRLHEVLGAITPLYEIILVD
                     DFSNDLSADVIAKMRDEDDRVKGIFHHQNIGQHPSILHGLMNSSGNVCIVMDCDLQDP
                     PEAITQMFMALGPTDDAVFALRKKTYNSAYQRYLSKLFYISLSLMSGTRLPGEIANYG
                     IYRRAVISKLFDARYRYFLFPLAVRKYARQSKTFEVEHARRFSGKSTYTIGKAGKLAI
                     TAILANSIFGFTFRKRTAQEIVGDNL"
     gene            complement(22255..22566)
                     /locus_tag="PLL28_04205"
     CDS             complement(22255..22566)
                     /locus_tag="PLL28_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HNF68553.1"
                     /translation="MSDAKEKNQLNIELSEEIAEGTYSNLAIITHSSAEIVIDFIRVM
                     PGVPKAKVKSRILMTPQHAKRLLRALSDNINKYESQYGDISDNEGFGGIPMNFGPTAK
                     A"
     gene            22645..>23598
                     /locus_tag="PLL28_04210"
     CDS             22645..>23598
                     /locus_tag="PLL28_04210"
                     /inference="COORDINATES: protein motif:HMM:NF040455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HNF68554.1"
                     /translation="MKIFPKLLVLTAMLATIGWLSSCRHEAGAEEEGTDLEDLSQLKP
                     VFYPLHIDSQSIAVFLQQYPLFDFHREEVFAFYKQRNDQSAWFNEYGIVDQGGYFMNL
                     LDHFSEEGLRDSVIYYSTLNRMYHTISDPAYSYNGADSLTTQFELMLTSTFFVYAQKV
                     WYGLSEKTTRSLDWYVTRKTVPSVSILDSILSGNASAFTAYEPLFPQYSLLKNALKKY
                     HAIENEPWDSIAIPADRKSILLGDDLPVLSEIKRRLFLLGDLNTSDSTTLYDSTTLAA
                     VMMFQERHGLFADGSMGPKFFTAINRTPAELIRRIEINMERC"
BASE COUNT         6946 a         5148 c         4517 g         6887 t
ORIGIN      
        1 caagaaatac caaattcctt ttcaaaaact gtcgctccac ggaaagtacc atcaccacca
       61 atggcaacac atccttcaat gccattgagc tttaaattat cgaaggccat ttttcgtcct
      121 tctgcagtca taaatctttc gcttcgcgcc gtttttagga tggttcctcc tcgttgaatg
      181 atattgctga catgataaga ttccatttta aagaaatctc catctatcat cccttcatat
      241 cctctgcgaa ttccatatac ttcaagtcca tgatataaac ctgtgcgcac tacagcgcga
      301 atacatgcat tcattcccgg tgagtcgcca ccggatgtca ttactgcaat ttttttcatg
      361 attagtttat tgcctgatca ggtacaaaaa taggatgtca atattaaact aacatctttt
      421 ttatcttgcg ggtttccaca aagtaagtat tatacatgat cgagcatatc ttttgtcatc
      481 gcatcaaggt cgtacgcgtg cttccagccc cagtcctgac gagcctgatg atcatcgata
      541 cttcccggcc aggtttcagc tattttttgc ctgaaatcga tttcgtaatc taatttgaaa
      601 tcaggcatat atttcctgat agactcccct accatggcag gattgaagct catagcggcg
      661 agattatatg acgaacgaac agaaattttg tctgcagatg cctccattaa ttcaactgtt
      721 gctctgatgg catcaggcat atacatcatt ggtaacatca tatcatgtgc cagaaaacag
      781 gtataggctc cttgtgcttt tgctttaaaa taaatgtcga ccgcataatc tgttgttcct
      841 ccgccgggtt gcgttttgta actgataatt ccggggtatc gcaaacttct tacatcgaga
      901 ccaaatttct gatgatacca ttcgcaccat ctttctcctg cctgcttgct gataccgtaa
      961 atggtatttg gctccatgat ggtgatctgc ggtgtatcca ctttcggtgt tgttggcccg
     1021 aaggcagcga tagaacttgg ccaatacatc ctgcgaatat ttttctctct ggcaagctca
     1081 agtgtattaa tcagcccttc catgttcaat tcccaagctt tatgtggaaa gcgctcgccc
     1141 gtggcagaaa gcatggctgc catattgtag atctgggtaa ctttatattt atcaataata
     1201 cctgcgatgc cggcttgatc caatacattg atctcttcaa atggtcccga ctctaacaat
     1261 tcacctccag gtgctttaat atccgctgct ataatatttg aaccaccata ctgatggcgc
     1321 agaaacattg tcaactcagc gccaatttgg ccacaggctc caattatcag tatcgtttct
     1381 cccggcatat cttgttgtta gggaaacaaa aatagcccta tggacaagaa ttgcaaattc
     1441 cgaaagcagg aagttataaa cgcttacctt tacggggtcg tagaatcttg atgaaaaaga
     1501 tattggtggt agtgggcaca cgcccgaatt ttattaaagt agccctgttt aaatccatgg
     1561 ctgccatgca tggttttgat ctcaggatcg tacataccgg tcaacattat gatgctgcca
     1621 tggctgatgt cttctttcga cagttggcat tgcaaccgga ttatttcctc gaacttcctt
     1681 ccttacagcc tgagcaacaa gtgattttga ttcaagccaa gttgaatcgc ctgatgggaa
     1741 gcacctttat gccagatctg gtaatggttg ttggcgatgt caattctacc agggcagcag
     1801 ctgaagcagc acacatttta aatattccta tcgcccatgt ggaaagtggt ttgcgcagct
     1861 tcgatcagtc catgcctgaa gagatcaatc gcattgttgc agataacctc gcatcactgt
     1921 tttttattac tgaacaaagt ggtatcgata accttcgcga agaaggtaaa cctgaaaata
     1981 aaatgcattt ggttgggaat accatgatcg acaccctggt tcattttgcg ccactgatcg
     2041 atcagcaaaa tattctggag caatggcaat tggaagaaaa aagatattac ctcgtaacct
     2101 tacatcggcc atctaatgtt gatgaaaaaa atggattaca acaggctata gaatgtttaa
     2161 attggctttc tgaagatcta cctgttgttt tcccaattca tccgcgtacg aaaaaacaat
     2221 ttctgcaatt tggattggat gaaaatattt cttccaacaa aaatatcatt caattaccac
     2281 cattggatta tttcggcttt caaaaattaa taaaatcagc aacagccgta attacagata
     2341 gtggcgggat tcaagaggag accacattct acggtgtgcc atgtttaacc atgcgcccaa
     2401 acacagagcg accagttact attaacctgg gaagcaatga attggtcgaa aataatattg
     2461 attcattaag gccatttatt cagcaaataa ggaccggtca ttataaaaat atctccattc
     2521 caccattatg ggatggacgt gctaccgaac gcatttttca gattcttgct gctttgcgat
     2581 gaagactgtc cagtaacctc gcgtattgtt ctgttaccga tttacgtgcg tatttttcta
     2641 tccctccact cttaacagaa agtttaccgg atttataatc ggcataccat gaagctatgg
     2701 tagattgcaa tccgtcaaca tcttttaaat caattaatat ccccgctccg gtttcttcta
     2761 gtattgttgc ggcatcaccc tttggatcac caattgccag tatcggacgt tttgctgcca
     2821 tgtattcgaa tagttttgca ggaatacgcc cgagaatatc ttgtgcatca ttaattaata
     2881 ataataaaat cgttgcacta cacatggcat ttatcgcttc tgcatgtgtt acttgcccgc
     2941 gattttctgc atatcctgtc aagccatatt tttccagaaa ctcatgtaca ctataatcat
     3001 tttttcctgt gaacacaatc cgcagatcat cacttaaacc cggcagcgtt ttgcagagat
     3061 tacttatcgc cagccataat gtttccggat ttcttgcttt gttgatcata cctacatgat
     3121 gcaatacaaa tcctttttcg ggatcaggat gtacagcagg aaaatcatcc tcatcatacc
     3181 catttgtgat gaccgtagaa ttgccattat tcaaaagatt aaaatcattt gcccactgca
     3241 tgcttacggt taccaaggca tctgcatttg tagctacttg tttttctaat tggtgatgac
     3301 gtttatcggc gaaagaggtt aaatgcaatt catgataata atcaatccgc gtccaagggt
     3361 ctctaaaatc ggcgatccat ggaatacctg ttcgctcctt taagcccata gctatcagat
     3421 gcatgctatg tggcggcccg gttgaaataa tggcatcgac aggatgcgat ttaacatagt
     3481 cagtcagaaa tcgaacagag ggttttatcc aaaagcgtcg ggcgtcagga atgaaaaggt
     3541 tacctctcac ccatattgcc agatgctgca aaagccctgt ttttttcttt tcctgcagga
     3601 aggctgcatt gatcttttct tcttttgaaa gccccaaaaa gcgcttgtac cacgaatatg
     3661 gctcccaaat tggtcgcttg atcacctcca gtcccgcagg aagtgtagcc gccaacgact
     3721 cgtcaataac aggcatttcg ccattttcag cggtataaat gacgggttcc caaccaaatt
     3781 gtcgcagata tcgtgccatt ttcacccagc gctgcacagc aacaccgcct gccggaggcc
     3841 aataataggt gataatgaga acgcgcttca aggtggttgg tttcagggct aaagttaaaa
     3901 caacttccac aaccctactt tccggccttt agctgtaact tcttaacttt aacgccgcaa
     3961 ataaaagcct atcatgcaaa aaaacaacgg ctctaagaaa ctgcttacct atctcgcagt
     4021 ttttgttttc ttcctcgcat tcagcgcatt gtatttttat ccgctattgc aaggtaaaat
     4081 tctgtttcag agtgatatca tgaactttgt cggcgtaagt aaagaagccg ttgatcatta
     4141 tgacactacc ggcgatgctg cactttggac caacagcatg tttggcggta tgcctactta
     4201 tcaggtgtca ttacctgaaa atggaaatat ttttgagaga ataaatattt atctcggcgc
     4261 atttcttcct cgccctgcca attatatgtt cattgcgctg gttacattct tcctgctgat
     4321 gcgcagtttt agagtgaaca catggttgag tatttccggt gcactctgct atgcgctggg
     4381 aacgtatctg attaccttca tggaggccgg ccataatact aaagtaaatg cactggcatt
     4441 gatgccgctg gtatttacag gattgcaata tctatggcaa cggaaatggt tattgggaag
     4501 cgcattgaca ttatttgcca tgacatgcga gatcactgca aaccaccctc aaattaatta
     4561 ttacatgttc ctcattattg ccatatgggg aatttcagaa ttagtatttg caatccgtga
     4621 aaaaacagtt ttacagtttg taaaaataac agcagtgatc attgctgtta cattaatggc
     4681 agttgctgcg aatacttcaa aattgtggac cacatacgag tattctaaag aatccatccg
     4741 cggaggtagt cagcttacca gtcagaaaac cgttcaggat gatggactta atcgcgatta
     4801 tgcttttgga tggagctctg gtgtttcgga atcattcagc attatgtatc cgaattttgc
     4861 cggtggcgga tccggtttct ccttcctcca aacccgcgat ggcgaaacta ttgacgggcc
     4921 aatgctgcca tttattcaac agctctatca aaaggatccg aagaaaactc aagaaatatt
     4981 aaactattcc ggacaagcag gaaaatattg gggcgacatg ccattcacga gcggaccaat
     5041 atatgtaggt tcaattttat gcttcttgtt tattcttggt gcattgcttg ccgataacag
     5101 aatgcgctgg tggttgattg cagctacggt attgtcgctg ttccttgggt ggggtaaaaa
     5161 tttcgaagca tttaattatt ggatgttcga tcatttcccg ctctacaata aattcagaac
     5221 cgtatcgatg gcaatgacca tgctctgctt tacgattcct gtgcttgcca tttatattgt
     5281 caataaattc ctgagtgata acaatgagat tacacatgag aaaaaatatt gggcgttgaa
     5341 atgggcttct cttgctacag gtgctttatc attgatattg atgatgtcgg gaagtttgtt
     5401 tgatttatta aagcctgttg aagaagaata tttaatgcaa gccaatagca tagaagcatc
     5461 cacattcttc gaaatgctga aggatgcacg cgctcagatg attcgcaccg atgttctgac
     5521 tacaacattc atcattgctc ttgcagcagg tgcactttgg ctttatctgc gcaaaaccat
     5581 tactgctaaa attgccatcg ccatcatttg cattttgccg gtgctagatt tgatgattgt
     5641 tgattctaaa tatctgggca aagacaatta tcaggaagct aatttttatg atcaacgact
     5701 gcgttcttcg ttgccggtga tcaaggataa agatccttat taccgcgtat ggaataccat
     5761 ggcagatcct gacaaagatg gcactacatc ttatctatat aaaagcattg gcggctacca
     5821 cggagcgaaa ttgcaacgct atcaagatat tattgatggc tatttgcata aaggcgatgt
     5881 gcatgtattg agtatgctca ataccaaata tgtgattggg cagcgtcaag gcaaaatcac
     5941 cttcgatcgc aatcctgccg ctttgggaaa tgtttggttt gtagattctg tgcgcatggt
     6001 aaaaactgct gatgaagaat ttaacggctt agccaacttc agtcctcaaa ataccgtaat
     6061 tgtaaatgaa gaatttaaaa atctgattcc cgcaaccacc atacaacgcg atagcaccgc
     6121 aagtattaag cttaaagctt atggtatgga tgagatcagc tatacatcga aaagtagtgt
     6181 agaaatgcct gcggttttct cggaaatatg gtatcgcggc aatcaggatt ggaaagccta
     6241 tatcgatgga aaatatgttg atcatttccg cgcaaattat ttgttgcgtg gattgtgggt
     6301 tcctgcaggt gaacatgaga ttgttttcaa atttgagccg gcttcatttg ccgcaggtaa
     6361 aaaagtttca acagcatctt cagggttgat attattatta gtggcgatag gttttttcct
     6421 cgccggaaga aaagcgattc aagaaaacaa acaaacagag gagcaagcct gacatgcagt
     6481 acgatcccgt taaacgcagt ctgggaaaag tatttaatac taccccattt ttgcgcatag
     6541 tattttataa aatgctcgac ctgcttttgt taagagcatg gcatatccat aaagctttgc
     6601 gtcaatggtc gaaaggaaaa aaggaaacag aacaacatat tcttgatgcc ggctcagggt
     6661 ttggtcaata cagctatttc ctcagcggca tgagcaacaa atggaaattc ctgtcagtcg
     6721 atgtcaaaga agaacaaatt ggcgattgca ataatttctt tcaaaaaatt ggcagaaaaa
     6781 atatacgttt tcaggtaaaa gatctgacaa cttttaccat tcccgatcat ttcgacctgg
     6841 tagtatgtgt agatgtaatg gaacacgtgt tagaagatgt gcaggtgttt aaaaattatc
     6901 atacttcatt aaaaacaggt ggcatggtgc tgatttctac tcctagcgat cagggcggct
     6961 cagatgtgca tgaagaaacc ggctcgtcat ttatcgaaga acatgttcgc gatgggtata
     7021 acattcagga aatacaagat aaactaacat ctgccggctt cactcgaact gaggctcact
     7081 acgcttacgg aactcccgga aaaatttcat ggcgactttc aatgaagtat cctttgcaat
     7141 tattaaatat ttcgaaactg ttttttatta ttctgcctgt ttattacatt attacttttc
     7201 cattctgttt attactgaat gcctgcgacg tggctatgtc gcataaaacg ggaacaggat
     7261 taattgtaaa ggcctggaaa taaactactt acaaatattc ttcgagcaac tccttcggaa
     7321 gtacagattt gatttgtttt tcatgcatat attgcagcac agccttgtat gcatcaagcc
     7381 atgaggtatc cgttctgtcg aaaaaataat tattgtgcca cagaatagag attactgcat
     7441 cttgcgggtt atgctctaaa aagtcgatta tcaaacgctc agcttcctta ccactttctt
     7501 ttcgatagta tcggtaagta gtatccataa tatggagtgg cacctcaata aaattatatt
     7561 cgcgcttttc tttaaaatga tatggcctga ccggcaatcc gtaactattt ctaaatccag
     7621 gtgcttctgc aaaccccatg gtgcaatcca tctgaattcc tgcagcctca atttcattga
     7681 atgttcccgg aagttcagtc aataaataat gattgcgatt tactggctct gcaaattccc
     7741 ttactgaatt gtaatcagca gtcattgtcg atttcccggc acttttatgt aacccgttta
     7801 cccatccctt ttccgatatg tgctgttgaa tggaccgaac tttactatca gacaaattat
     7861 aatccgcatt gttgatcaaa cgtgttccgc gacctgaacg cgccaaccaa aaaaacaccg
     7921 atttcacatc gtatgcatct tcaatttgca tgattttatc caaaagcaga tgatcaggcg
     7981 tgcgcacata atgattaaaa atcaattgca tcaatgcgcc tacctttcca tgttttaaaa
     8041 gccatttacc attttctgcc aaagcgccgt aagcactatc aatgtcatgc gacagaaaaa
     8101 ccacagatgc atgctctttc ttgctaacca attgcttcaa ttttggagtc tgctcgtata
     8161 ataatgtgaa atattgcgct accaggtttt tatcgataca atcaaaatga tgctgaatac
     8221 tattggaaaa cggaaaacga ccatattgat ccggatgata atcggtgtat tcctgtaagt
     8281 aggatagcaa gtaaaaacaa gtacttaaat aatccggttt gtttgatgcc gtgtagtgca
     8341 gtaattcctt gcgaaagtaa gatttaaaat gacaatcacc actttgatac gtcattctga
     8401 aaaaatgaga aacctgtatg tcacccatgc catgttcggc aattagcata tcttcattcc
     8461 catcaacaaa ttgcacatta aatccgatat acttagcaaa caccttcatc acccaagtgt
     8521 attccgacac cagaggcgac tctattctgc atgtaatggt tcgcatttgc ataaacaaaa
     8581 aaccgtcatc aaagatattc aaacctaaga tgacggtcta aattagttga ttttatcaga
     8641 tatcaaattt tattccctga gccaatggca actgcgtaga ataattgata gtattggttt
     8701 gacgacgcat atatgctttc cacgcatcac taccactttc gcgaccgcca ccggtttctt
     8761 tttcaccacc gaaggctcca ccgatctcag caccgcttgt gccgatattc acattcgcaa
     8821 taccgcaatc agagccttgt tgtgaaagga aatattctgt ctcataaata ttggtcgaca
     8881 taatagaaga tgaaagcccc tgaggaacac cattttgcat gtcaagcgct tcctcgatag
     8941 ttgaatattt catcacatat aagatcggag caaatgtttc gtgctgaaca attttgaaat
     9001 tgtttgaagc ttcgatgata catggcttca cataacatcc gctttcatac ccttcaccgg
     9061 aacaaacgcc gccttcaaca atcattttcc cgccctcttc catagcttga gcaattgctt
     9121 tttcataatc ttcaacagct tttctatcaa tcagcggtcc tacatgatta ttttgatcca
     9181 atggatttcc tattcgcaat tgcgcatatg atttcttcaa cagattcaac acatctgcat
     9241 aaacactttc gtgaatgata agtcgacgag tggtagtaca tcgttgacct gcagttccaa
     9301 cagcaccaaa gattgctgca cgaatggcaa tattcatatt tgcatgttca gaaatgataa
     9361 tggcattgtt accccccaat tcctgaatca cgcgaccaag acgttcgcca acaataccac
     9421 cgatgcgttt tccaacacgt gtagatcctg taacagaaat caatggaaca cgtgcgtctt
     9481 tagccatttc ttcgccaatt gaactatgcg ataccatgag gcaagaaacg ccttcaggaa
     9541 cattattggc cttaaatact tcagctataa ttttttgtgt ggcgatggcg ctcaacggca
     9601 ccttagaact tggcttccaa atgcaaacat ctccacaaac aaaagctaat gcagcattcc
     9661 agctccatac ggcaaccgga aagttgaatg cagaaataac gccaacaata cccagcggat
     9721 gccactgttc atacatacga tgcaaagggc gttcactgtg catagacaat ccatacagct
     9781 gacggcttaa tccaacagca aaatcacata tatctatcat ttcctgtacc tcgccaaggc
     9841 cttcctgcaa gcttttaccc atttcataac taactagctt tccgaggtcc tgcttatgct
     9901 cgcggagctt ctctcccaac tgacgcacaa cttcaccgcg ctttggagcc ggccacatgc
     9961 gccattcttt gaatgcagca gcagcagttt gcatgatctg ctcatactct tcgcgtgagg
    10021 ctgaagtaca cgacgaaatc agcgaaccat caaccggact gaagctttca acgccatttc
    10081 cctcgccttt caaccaattt acgccggtgc ttacaccgct attattcgat agaatgccta
    10141 aggctttcaa ttcggcagct atggcatttc cctgtatcat tgtatccatt tagctatatt
    10201 tagggtgcaa aattacgaaa tttcggggtg aatgaacccc gttgaactga cctagacagt
    10261 atcgtcataa ggcgtattca acagtgcttt accgattaaa ctaatattaa atccgtttag
    10321 ttcgaattta tattctatta cttacattaa ccggtgtggt atcacgtann nnnnnnnnnn
    10381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10441 nnnnnnnnnn nnnnnnnnnn nnnnnnnncg tttagttcga atttatattc tattacttac
    10501 attaaccggt gtggtatcac gtacggaagc acaagtaaat gtgccgggca atggtgcaat
    10561 atggacttat accattgatg acgtcgattt aactcacggc acttgtttct atcgctttaa
    10621 atatgtgctt tatgcagaca ttgacagcac tattaatgga tataattata cacttctaaa
    10681 attaaagcaa acgttatatt acacttttga tccatttaat caattctatt gctccataga
    10741 tacttttgat catgatttag gaatatttgg tggaatacgg tcacttgatg agaagacata
    10801 tttctatggg ttgaacaacc ctctacaaat tatcctcggt ctggatgtcc cggatactac
    10861 cgacgtattg gtttacgact tctctaagat accgggagac accttaccac gtaaagatta
    10921 ttacggatac attatgtatg actccattaa agaaactagc tatgtaacac ttgccgatgg
    10981 aatcataaga aaacagcaaa ctgtcgttta caagtcctat actgtcggaa tctcaggcga
    11041 tacaattctt gccaatagtg atcaaaacat ttatgttgaa ggcattggag atattgaaaa
    11101 cggattattt tttccaatct caccccaata ttcaagttat tacgtaagtt taaattgcta
    11161 taaggaaaat gaaatatatt tattgggtga caatagttgt gaaaatgaag acgcacaatt
    11221 tgaaactcca actttagagg gaaaaacaat aattgcatat cctaacccat tcaaggaata
    11281 ttttagatac gaagtgccgt atgatcattc taccatccgt ttgtatacat tagatggcaa
    11341 gcttgtatta gagcaggtga ctgatttgcc aaatggatat attagtactc gccatattcc
    11401 aaatggcgct tatatcgtaa cttgcacagc tagcgatgga aaagttgcat atgacattat
    11461 ttgtaaggcc gcaacatatt aaatacctcc aaaatttatt aaatacaatt aatatcaaca
    11521 gatcaattta ataaactctg aaaaatcaac caaatgggca cccgaatcaa cgccgtagat
    11581 caatacatcc taaaatcaca ggcattcgct caaccaatat tgaatcactt acgcgatctg
    11641 gtgcatacag tatgcccgga tgttgaagag aaaattaaat gggggatgcc atttttcgat
    11701 tacaagaatg atatgctgtg tcatatggca tccttcaaag ctcatgcagt ttttagtttc
    11761 tggaaggcgc aattgatgaa agatcccata ttgatggaaa atgccaattc ggagcaggcc
    11821 atgggccatt taggtcgcat aacctcgtta aaagaccttc cttccgacaa gcaactaaga
    11881 gcctggatca aagaggcaat ggtgctgaat gataagggca taaaaattgt aaaaccgaaa
    11941 tcaacaggcg aaaaacctac tgtcgaaatt ccggaaatgc tgatcaaagc acttaaaaag
    12001 aataaatcgg catgggctgt atggcagaag gctactcccg gatatcaaaa agaatatgcc
    12061 aactggatat cagatgcaaa aacggaagcc actcagatca aacgaaccaa tactgcagtt
    12121 gaatggatag cagaaggaaa gatccgcaac tggaaatatc aataaaggaa agaggagaga
    12181 ggagagagga gagaggagaa aggggtgagt aggtcagtgg gtgagtgggt gagtgggtga
    12241 gtttacctcg ccgagtaggc gggtgagtta gtaggcaaat aaaatgggct aagacatagt
    12301 cttagcccat tttatttgaa tcctccgtac cacactcacg attcacgagt cacgattcac
    12361 gattcacgat ctactaccgc ctatcggcga ggcaagcccg ccaaatgcga ggcaagctca
    12421 ctattctacc gccaacttgc gcatcatttc gaatacattc aatgcgttga tgctaagctg
    12481 gaacgacatt ttttctccat aggaggcatc atcggcaaac tgatcagcaa gatcgctgct
    12541 catgacaaat ggaagatata gactgaagat atctagaggc agtcctatct tcaaaccggc
    12601 atcatactga aatcctgaga tacctgtcac actttctctc aacccgaaat aacctgcatc
    12661 tgcaaataat gataccagag gtactttagg aattgtcaat tcaaaattcg ctgcacccat
    12721 ataactcctg gatgtgaatg cgtaattata tacaggcact ttaaagaatc catcagtagc
    12781 tgtcatttgc gtactaagga aaccagacga ttcatttcta cctagaaaaa catcatcata
    12841 agttgcatca taatatccgt tatttcctgc taaagtgaat gactggtttt catcaccacc
    12901 atcttgtaaa aatgctcctc cgaataaacg aacattcata ccttttttca ttccgggata
    12961 gtgaatggta gtgtgatatg ttgcagaaag cttagtaaag ccctcccctg ccgttaacaa
    13021 tgcgtcaact ttttgcggga aaaatgttct gtcgtaagct aagtggtatc ctaattcata
    13081 ttccattcgc gaagcagacg cagttccggt gatatcgccg ttgatatcat ataagaagta
    13141 caaatcgtct tcctgaataa aaatagatct ggcgcttaat cgttgagtaa gttttgatcg
    13201 catggattta ggatgcagat ctgcctctat tttgctttcc aattttaaat attgaggctt
    13261 gtattgcgcc tggtcaaaca aggcattgat ataaaattca tcataggtat atcgcgctgc
    13321 actttcagaa atgcgaatcc tatcgaacaa accgttatcc ggataaaaat tcaatccaac
    13381 gctcccctga cctacaggct caccggaata aatgctatac ataggcgcca gcacatattc
    13441 tattttaggc gttggcatgg tactgttcca tattcccaag cctagcatcc atctatcatg
    13501 atcgttccac cctactaatg gcattacact tattgtggtg cgattcggat tgtcggcacc
    13561 gaatccaaac agccttacct gcaaaggttc cactcgttta aacaagcccg atgtttttat
    13621 ggtattattt tcacgttccg tttcaggcat tttctttctt gcatctatgc gcaatgcagt
    13681 aacatgcata ttttcattgt aattcaaata taccgtctga ctacctgtga atccgcgaaa
    13741 ccaatcggtg cggataattg aatctcccga catcaaagca atcgtaaatg ccgcaggaat
    13801 atcactgttg tttttcacgg ttacagccat cgctgttttg ctctttaata agtgcttgat
    13861 actgtaatcc ggcccgcgat cactctgcaa caattgctgg aaaaaccatc ctaacgaatc
    13921 accggtttca gattcaaaca cggcttgcat atcttcagga tatacgtgtt taaattgata
    13981 ctggcgatag tatttgcgca tgatctgatc atatttatcc gtgccaagta catcttccaa
    14041 ataacggaaa tcataaccgg tttgcatata cattaccaaa ccataattcg cacttgtata
    14101 atatttcgaa tgcaaatcaa ccggttgata acgattttgt ttttgagtag tctggttaat
    14161 atgccataat gtttcattta tatctgatgt atcaacattt aatgccgcta atgcaaaatt
    14221 tggtatccca agctgatttg caggctcata ataggtagcc atatatcggt tctcataaaa
    14281 tgtattcatc ccttcatcca tccaaccatg ctcacgctca ttgcttccaa gaattccata
    14341 aaaccaatta tgcccaacct catgcgtaat tacattatcc agcattttcg catcaaattt
    14401 tcctgtgata acagtaatgg ttggatattc cattccaccg gcaccttcaa cttccagcga
    14461 gccatcaact gcctgcgcaa cattccatgg atattcaccc acattgtcag aataaaatgc
    14521 tactgctcta tcaacatact ctgcagcctt caaccattct tgtccatgct catcattaaa
    14581 atacgcataa gtggttacca ctcgctccga ctccggaagc gtaacactgc ttttcattac
    14641 attaaatcgc ttgtcggcaa accatgcaaa atcatgcgca tctttcaaca tgtattccaa
    14701 tgttttggtt gttggtgaag aaggcggaaa actcaaatct ttagtttcaa atgtttttgt
    14761 agccgttacc gcagcgatac tatctaaaaa tgtcacttca cttgcagtca gtaaatcacc
    14821 actcgcgcca acaacataat tttttggaac agtaataaat acgtggaaat ttccgaattc
    14881 actgtaaaat tctccctggt caagatatgg aatggcatgc cagcccttgc gatcgtatac
    14941 tgccggtttc ggataccatt gtgtgatcaa atattgctgt tcatcatgac ccatgcgcga
    15001 aaaacaattc ggcaccttaa cacgaaatgg cgttttgata ttaatggttt gtcccggcga
    15061 taatggttta ttcaaagtaa tttttgcaat atccggcgtc tcttcatcat aggtaagtgt
    15121 aacttcttca ccattgactt taaagttgag attatctatg aagccacgtt cagactcatc
    15181 ggcgaagtga aatttcacat cccccgactc tagcttttgt ttagcaaatg ccgtgctgtt
    15241 gtccttatac gcatttggcc aaaggtgtat ccaaatctcg tttaatacat cgggtgaatt
    15301 attcgtatac cgcatagcga tgctgccatt tagcatatga tttacatcgt cgagcactac
    15361 attgatctcg taatccacat gctgctgcca ataacttgct tgctgagcat atacagattg
    15421 ttgtaacttg agcgtcaata gtaaagagag aatcagggag agttttttca tccttgtaga
    15481 atttttgtaa agataaggtt cagttttctg cttcctaaat gaggcaaaag cccaaaatgt
    15541 tacaaaacac ctcttttttc gcgttcagga aactattcag gtcatttgta aatttgcaat
    15601 aatatgatgg accatgattt taacgaacaa gaccctacgg ccattgcaga gttgatcgaa
    15661 cagttcgaaa ggtacctcaa agacaatgaa tacgcctatt tcgacgagga gagcctggag
    15721 ctgatcctcg aatactatga aatgcgcaac ctcaacgagc gcgccgaggc tgtgattgcc
    15781 ttcgctttgg aacaaaatcc tttttcctcc gactttctgg tccgcaaggc ggagtacctc
    15841 ctcaacagaa aacgctatca ggaagccatg gataccctcg acaaagccat gctcttcgat
    15901 agtcgcgaga tcgatatcta ccttttgaga tcggatatct atgccgaaca aaataaactt
    15961 acagaggcag aagctatcct gctggaagcc cttgaaatag ccgatgaaga ggataaagac
    16021 gccgtatatg ccgaacttag cgatatctac gagattcaag agcagttcga taaggcattc
    16081 gactgtctgg caaaagcatt gtccctgaac ccgtcctctg aagaagcact ctataaactg
    16141 gcccatatcg tagatatgac cgacaggtat gatgattctg tttcgataca tacaggtatc
    16201 atcgacaaag acccatacgc ctggctggca tggtataacc ttggtagagc ctatatcgga
    16261 ctgggacttt acgagaaggc tatggaggct tttgaatatg taatggccat agacgagcat
    16321 ttcgaccttg tttaccgcga tgccgctgac ctgtattatc gcatggaagc ctttgaaaaa
    16381 gcaatttcta tgtttgagat tgcgcatgaa aagtccggag gatacgaaga ttacagcttc
    16441 cgaataggct tatgccacga gcgcttgaac aatttcaagt cggcccgctt ccaataccgc
    16501 aaggccacgc gccaggatcc tttcatgcac gaggctttct tccgtattgg agaaacctat
    16561 cgcctagaag aacgcttgga acccgccctg gtgaactaca aaaaggcact tcaggttgac
    16621 gataccaatg aagattacat ctgcaccatc atcagcatat acaaaatgct tgatagagag
    16681 gatgatgtgc ttgaacatct tcgtatcctg gtgaatgttc gtgctgatat cctcagttac
    16741 tggctcgatt ttctaattta tctgttcgat ataggtcaat tcgaaaaaac cattgaaata
    16801 gctgatgaag ccatacgaag aagcggtcat ttcgccgaat actactatat ccaggctgca
    16861 gcactatcgg cgcttggcga tggaaagagc gcattggttc tgttagaaca tgcacttacc
    16921 attgactatg gacgtcatac catactatat gagttgaatc gcgagattgc ccttctacct
    16981 gccttccagc aagtgatcct gcagttcgca ccacgctctt gatcagtaag atatgctgtc
    17041 tattgcgtat ttttgcagct gtaaccaata gttctaagta catgaatttt aatttaaccc
    17101 gacttccgga acgcacatcg aaaccgagga aggaaggctt gtgcatggta atggacaaag
    17161 gccttagcgt aagagaagcg gaagacctcc tttcagtttc tgaaccttat atcgatttaa
    17221 taaaattggg atttggcacc gccttcgtaa ctcccaatat cgaaaataaa attgccgttt
    17281 ataaaaatgc aggcattccg acttatttcg gtggtacatt gtttgaagca tttttagtgc
    17341 gcggacaatt tgaagattat ctgcgtctgc tcgaaaaata taaaatgtcg tacgcagaag
    17401 taagcgacgg ttcaatagaa ttgaatcacg atgaaaaatg cggctacatc aacaggcttt
    17461 ctaaacacgt taccgtatta agtgaggtag gatcaaaaga tgaaaataaa atgatcgcac
    17521 cttacaaatg gatcgagtta atgaagaaag aaatcgaagc aggaagctat aaagtaattg
    17581 cagaagcacg cgaaagtgga accgttggaa tttatcattc taaaggtgaa gtgcgcagcg
    17641 atttgattga tgaaattctt acacaagttc cgcaagatca gattatttgg gaagcacctt
    17701 taaaaggaca acaagtgtac ttcattaaac tgctcggcgc caacgtaaat cttggaaata
    17761 ttccacctaa cgaagttatt cctcttgaga cattgcgtct tggacttcgc ggtgatacct
    17821 tccaccattt cctgacgtaa tctatcctga atggaatttc tgcacacgtt tatcgatact
    17881 ttcctgcacc ttgatcaata cctggaaagc tggatcgata cctaccaaaa ctggatatat
    17941 gttatcctgt ttgctatcat ttttattgaa acaggtattg taatctggcc ctggttgccg
    18001 ggtgattcat tactttttgc agcaggtgca ttttgtgcaa tcggatcgct gaatattttc
    18061 atcatcatac cattgctatt tgttgccgca gtacttggcg ataccagcaa ttatttcata
    18121 ggaaaatatt ttggtgaaag agccatgcgt gtaaaatggc gcggtaagcc gattctgaaa
    18181 ccggaacacc tcgcaaaaac ccacgctttc tatgaaaaat atggcgctaa aactatcgtg
    18241 ctcgctcgct ttgttcccat tgtgcgcact atcgctccat ttgttgcagg tatgggaacc
    18301 atgacctata agacattctt ttcatacaac attatcggcg caatactttg gattccttct
    18361 atcacagtat taggatatat ttttggacaa aacgaattcg tgaaacacaa ctttgagata
    18421 gtagttttag ccattatctt catttctgtg ttgccaatat tttatgagct gttcaaagcg
    18481 cgatttaaaa ggtcgaaaaa taattaatca tttgacctat gaaacacttc gggctgatcg
    18541 gatttccttt atctcattct ttttctgaaa catatttcag agagaaattt tcacgggaag
    18601 aaatagagga ttgcatatac gaattatttc cactcgaaaa tattgaagat gtgcgtttac
    18661 tgtttgaagt aaacaaagat ttggtgggat tgaatgtcac tattccatac aaagaaagtg
    18721 ttattgaata tctcgatgat cttgatcccg ttgctcaaaa aatcggtgca gttaattgta
    18781 ttcatatcga tgaaattcaa cgcaccggat acaataccga ctggatggga tttcgagatg
    18841 caataaaacc tttcctgaac aaacctcatc aacatgcttt ggttttggga acaggtggtg
    18901 catctaaagc tgttgtttat gccctccgtg aattaggatt gcatgttaca ctcgtttccc
    18961 gctcttcggg gaataaaaaa attgcttatg gcgatataac ttctgatatt atcgcacatc
    19021 atacggtgat tgtaaattgc acaccacttg gcatgtaccc aaatacaaac gagtgccctg
    19081 atattcctta cgatttaatc actgatcaac acttgctgtt tgatttgatt tataatcccg
    19141 gggaaacatt atttcttcaa aaaggaaaag ctaaaggtgc aaccatcagc aatggacttg
    19201 aaatgttgcg actgcaagca gaatacgcct gggaaatatg gaatcagcaa agtgaagaat
    19261 cggaagccga aatgtaattg gcacgaatca acataatgga aattgctttc catcgcccaa
    19321 taattgaaag gaaaatctgc ttaaatttta tttgttatcc tgattgaaat aacatttgat
    19381 cgtattcacc ttacgctgaa attccatgtg gtaggtgaat gtgagcatgc ctccaaaaat
    19441 gtatgagtca ttattggtag gatcgccgcg accgtattcg ggttgagtcc actcaggaat
    19501 tgtatgatcg gatggatctg ataatgttgc cgccattaat gcggtttcaa gatcataatg
    19561 caaataataa taatccggat tgggataata accacttaca tcgtcgatat aatctgtatt
    19621 ggtaaaacga gcagccattt caaaacccaa aacccagtct ctatcagtga tatatctgaa
    19681 tcctcctcca acaggaaaac atactgctat tttcgaatat ttctgacgat caggatattc
    19741 aggtaatcct tgtccttcag tacctagtgg ctgaagattc acccattcgc cattgtattg
    19801 tgcctgcggg ttaaaataaa atgcaccaac acctccatag atatatggcg tccatttctt
    19861 tttcttcaca ccggtaaatc tgaaagcact taattctaca gaaccacttg cttcaacaat
    19921 tggactccgg aagcttagat ttctataatg acggaattca ttatccgtca atgcatcatc
    19981 cccttttaaa atggaggcat aagcattaaa acgaaaagca gttcttggaa tgatctcctg
    20041 acgatacaca aaaccaagtg ccggtctggt aatctgagga tcaatatccc accagaaggc
    20101 gcggccgaca tcgtcagaac ccccgagatc tccgagaaaa ctcgaaaaac caaggtttgc
    20161 tccaatctca cgagaaagcg tgatcggctg ggcatataag tgtgtggtca gcaagagcat
    20221 aacaagccct gacgtagaga attttatgaa ttcttttttt aacattcggg tctgttgctt
    20281 gctagggcga tacgaaactc agcatttttt taacaaattc aaaatattag atatgtgaca
    20341 tgaaaaaatt catgctgaaa tcatatttca cccagattat gcataaaacg attttaacac
    20401 ttcagttatt ttctccaccc cacttaggct taggtcatgg aacaacggca aacgcagtaa
    20461 atttcgctca aacctaagtg cattgggcat ttcacgacca tcatgcttct cggaatagta
    20521 cggtgactta tgcaagcaca aataatgtga aactgccata actccctgtt tttcaagaaa
    20581 ttgtatcaat ttcccccttt ctatagaatc cttacaaatt acagctacga ggtgatgatt
    20641 atgtgtgctt cccgcttgct cctccaataa ttcaaaatat ccggccttct gcatttccat
    20701 cagaggctcc cgatatgcct gccataacac tgctctttgt gacctgattc cggaataatg
    20761 ctccaattgc ccccaaagcc acgcagcttg taactcagag ggcaaaaaac tcgatcccag
    20821 atccatccac tcatatttgg aggcctcccc tctgaaaaat gccgacctgt ttgttccttt
    20881 ttcccaaatc acctccgccc tatctgcata acgctcgtcg ttcactgcta atagtcctcc
    20941 ttctccgcaa tgcacatttt ttgtttcatg aaaactaaaa gcaccaaaat gccctatcgt
    21001 tccacataac acaccatgat acctgctgcc aatagcctgc gcggcatcct caattacaaa
    21061 caaacctttt ctgattgcca aatcaagaac cggctgcata tcaaccgaat aaccaccata
    21121 atgcacaact actattgctt ttgtttttgc attcaccaag ggctctatat cctcaacctg
    21181 cattaccgga tgatcaggct tactatctac aaattttata accgcaccgc gaagcgcaaa
    21241 tgcgtttgct gtggaaacga atgtatacgt tggcattatt atttcatcac cgggcttgat
    21301 attacaaagc aaggccgaca tttctaatgc tcccgtgcag gaatgcgtca acaaagcacg
    21361 atgcacaccg aaatgctgct cgaaataatg atggcattga tgtgtatata aaccattgcc
    21421 cgacaacttg cctgtttcaa cagccgccaa catatgcgta tgttcactgc cgagtgtaga
    21481 tggtttgtta aatggaatca tgcctataaa ttatcgccca caatttcctg tgccgtgcgc
    21541 ttcctaaaag taaaaccaaa aatagaattt gccaatatcg ccgtaatggc caatttgcct
    21601 gccttaccaa tggtgtatgt cgatttacca ctaaatcgcc gtgcatgctc tacttcaaat
    21661 gtttttgatt gccgtgcata tttgcgtacc gccagcggga ataaaaaata gcgataacga
    21721 gcatcaaaca atttacttat aaccgcccga cggtaaattc cataatttgc tatttcaccc
    21781 ggaagccgag tacctgacat cagcgacaaa ctgatataaa ataatttaga taaatatcgc
    21841 tggtaggcag aattatatgt tttcttcctc aaggcaaaca cggcgtcatc agtgggaccc
    21901 aatgccataa acatttgtgt tattgcttcc ggaggatcct gcagatcgca atccataaca
    21961 atacaaacgt tcccgcttga gttcatcaat ccatgcaaaa tagaagggtg ctgtccgata
    22021 ttttgatggt gaaaaatacc ttttactcta tcgtcctcat cacgcatttt tgcaataaca
    22081 tcagcactca agtcattgct gaaatcatct actaaaatga tttcatacag cggtgttatt
    22141 gcacctagta cctcgtgtaa tcgctgcact aattccggaa cattgccttc actgttatat
    22201 acaggactga taacagaaat ctccggcgtc tgctccataa gaatcaattc acgatcaagc
    22261 ctttgcagtt ggcccgaagt tcatcggtat accaccaaaa ccttcattgt cggaaatatc
    22321 gccgtattgg ctttcatact tattgatatt atctgacaag gcacgcaata aacgctttgc
    22381 atgctgaggt gtcatcaaaa tgcggctttt cactttagcc tttggcacac cgggcattac
    22441 tctgatgaaa tctataacta tctcagcgct ggaatgcgta atgatggcaa ggtttgaata
    22501 ggtgccttca gcaatttcct cactcaattc gatgttgagt tggttttttt cttttgcgtc
    22561 gctcataatt tccttttgat gtaaaagtac ccgaaaatga gggcaatcag aaatatttaa
    22621 gaacttcgta aaaaaattac cgctatgaag atcttcccca aacttctggt tttgactgcc
    22681 atgctggcta ccataggatg gcttagctct tgccgacatg aggccggggc tgaagaagaa
    22741 ggcacagact tagaggacct ttcgcagctc aaaccggtat tttaccccct gcatatcgac
    22801 tctcaatcca tagcggtttt tttacaacaa taccctcttt ttgattttca tcgggaggaa
    22861 gtatttgcct tttataaaca aagaaatgac caatcggcat ggtttaatga gtatggcatt
    22921 gttgatcagg gcggttattt catgaacctg ctcgatcatt tcagcgagga aggattgcgc
    22981 gattccgtga tatattacag caccctcaat cgcatgtatc acaccatcag tgatccggct
    23041 tattcataca atggtgccga tagtcttacc acacagtttg aattgatgct aacatcaaca
    23101 ttttttgtct atgcgcagaa agtatggtac ggactcagtg aaaaaacaac gcgctcatta
    23161 gattggtatg ttaccagaaa aactgttcca tcagtttcta ttttagattc catactcagt
    23221 ggtaatgcca gtgcattcac tgcatatgaa ccactgttcc cacaatattc tttattgaaa
    23281 aacgcactta aaaaatatca cgcaatcgaa aatgaaccat gggattccat tgctattcct
    23341 gcagatagaa aatcaatttt gctcggcgat gatttacctg tgctgtcgga aataaaacgc
    23401 cgattgtttt tgcttggcga tttaaataca tctgattcaa caacacttta cgacagcact
    23461 acactagctg cagtaatgat gtttcaggaa agacacggcc tttttgccga cggttctatg
    23521 ggacctaaat tttttactgc aattaataga acaccggctg aattaatcag acgcattgaa
    23581 ataaatatgg aaagatgc
//
LOCUS       DAOQHY010000031        23595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_945_length_23595_cov_6.125319, whole genome shotgun sequence.
ACCESSION   DAOQHY010000031 DAOQHY010000000
VERSION     DAOQHY010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 23595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23595
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_945_length_23595_cov_6.125319"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="PLL28_04215"
     CDS             complement(<1..788)
                     /locus_tag="PLL28_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68555.1"
                     /translation="MKRTFKHVIFSLLMLCVAHASYAATITVLPGNGSTSGNGRAPQG
                     SRLYINTLYYISPSEMTTSGYGSDQVTTVGWTWQAGSPQNVTTTGSLKVYLQNSADLA
                     YSKGTTFSTVGMTKVIDGTITITNSGVQFSIDVPVGGTGTSVFNTVAGQGLYVAFEYQ
                     TTTALALPLGAPTVLCTNTVANSLGTYQSQTAYGTPMTLSGFRPETRLGNPLSDKANV
                     QAVYCLANTPIPYGNPSGLSAKIKNESTSAETFTVNFTVKEKVSG"
     gene            1053..2555
                     /gene="cysS"
                     /locus_tag="PLL28_04220"
     CDS             1053..2555
                     /gene="cysS"
                     /locus_tag="PLL28_04220"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNF68556.1"
                     /translation="METALFHQNKLMVYNTLSREKEHFIPLHAPFVGMYACGPTVYSE
                     PHLGNCRSFVMYDLMVRYLTHLGFKVRYLRNITDAGHVESTSGETGDRIGKQARLEQV
                     EPMEIVQKYTVKFHWIMDLLNCLPPSIEPTATGHIVEQIEMTKTILANGYAYETDEAI
                     YFDVEKFSKDYPYTQLSGRNLEELLNNTRELGGQSSKKGRLDFALMIKAKPEHIMRWP
                     SPWGDVYPGWHIECSAMSTKYLGKTFDIHCGGMDLIPTHHTNEIAQSIACNHTTPVKY
                     WLHPNMLTVNGQKMSKSLGNAFLPVELFEGTHPLLDKGYSPMTVRFSMLQAHYRSTLD
                     FSNDALKAAEKGYKRLMSAMNLLKGITVSDTVNADEEAAVNKILLGVYEGMNNDFNAA
                     QAIAELNEAATIINTWNDKQNKKASIQAFTLSRLQQTYRAFLIDIFGLQEETENDNSK
                     LSGVMDLVIDIRKDARGKKDFATSDKIRDALQSVGIQLKDSKDGTTWEGN"
     gene            2684..3484
                     /locus_tag="PLL28_04225"
     CDS             2684..3484
                     /locus_tag="PLL28_04225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF68557.1"
                     /translation="MSQTPFDLAAPEYDSRFTETPAGKAQRDMVWRFLHKQLPPSPAN
                     ILEINCGTGEDAVHLATAGFHVTATDASENMLAEAGKKFASVRMNVHSYVWDVRTPFI
                     GTEKFNVIFSNFGGLNCLSPSELRSLFKRMQSQLSENGKLIIVVMGRNCPWERLLFSL
                     KGNFKQAKRRKSKNAVIAKTGGENSLPVWYYSIEEIVEFAGDNFNLVFSRPIGLCIPP
                     VYGKKAFWHNTMLIALLRNIETAIGGLKLFRNSGDHLFITLQKNEADQ"
     gene            complement(3509..4483)
                     /locus_tag="PLL28_04230"
     CDS             complement(3509..4483)
                     /locus_tag="PLL28_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68558.1"
                     /translation="MSRLNISGSVSYLDTTPAKNATVRIIDQDDRSSDNVILTATTDS
                     RGKFVGRTIDWKDEPDGFWPWEHDFMNLTFEVTDSIGRRHTGPFIYVNAAISVPILLP
                     FTKPAIRSENRALLVLADLSQVLTGTLKNFRPLYQFSESNGVNLVNANLRGYYHEIKV
                     LGPGDVTLDRIVSNLSRMTGNSNIWEVDLIVFLHGGRDSLTLSNDTVHMTTIVNAIRD
                     ANLPASKLRMVYSTACYGASQADNWVSAGFNCASGSKACNANGAVELLPFLLKWREGK
                     TFGTCISESENLVLSAPFDAAARLMLPDQVVNSDKDVIGDGTITISSF"
     gene            complement(4759..5598)
                     /locus_tag="PLL28_04235"
     CDS             complement(4759..5598)
                     /locus_tag="PLL28_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68559.1"
                     /translation="MKTSKLFLAIFLLIPLWGSSQDALNYQAVIRNPSNELVINTEIK
                     FEFGILVGGSQVYAERHAVYTDMNGQLSLKIGNGLVISGVFDSIKWGSDYMEILIRCD
                     IDGDDIYDIITTEALNSVPYAHYAQTAVSAQTSKDNKWAGSGEMIYTISPETNVGIGT
                     TAPMTKLSVNGAISLYPEPDVIVITYDNYFLEVGDRSYLQLQSDDTLAANRTIILGDG
                     ITPGQIIILQGFMMSDTFLELSDGDASNINIPQTFIIGNDDVVTLIWSGISWVGVSIS
                     DNE"
     gene            complement(5605..6126)
                     /locus_tag="PLL28_04240"
     CDS             complement(5605..6126)
                     /locus_tag="PLL28_04240"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68560.1"
                     /translation="MKGIFYVIVCYCYLITNVDGQTISSFVIATDGQTFAAHGNISYT
                     IGETCIRTFDKYGYTLEEGFQRVTTIPEPALDTTIFEADNVTGLSPEIVISCYPNPAT
                     ALITIECDAMLISGAEGTIYNLMGAEVMQFKFEHEYSTQLDVTSLSAGQYLVAIQSPK
                     GFVTAKQIFTILK"
     gene            complement(6294..6941)
                     /locus_tag="PLL28_04245"
     CDS             complement(6294..6941)
                     /locus_tag="PLL28_04245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF68561.1"
                     /translation="MSIKVCVFDDHRRVRDAMSALLKGTPGFEWGGGFPNAENVVKDV
                     EKADPDVVLMDIEMPGLSGIDAVKMLHAKFPDVRIIMQTSFDEDRKIFYSICYGANGY
                     LLKNTPPHQVLEAIKEVYEGGAPMTPSIARRVVELFRSAAMEKPMAEKDYQLSDREFE
                     VLQLLAKGKSYKLIGEALFISAETVHSHVKKIYDKLQVASKTEAVAKALQENLIS"
     gene            complement(7006..8883)
                     /locus_tag="PLL28_04250"
     CDS             complement(7006..8883)
                     /locus_tag="PLL28_04250"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNF68562.1"
                     /translation="MRSGAFYLFLLLVSYSFLMNAGTGNDTARINALNKMSFDLIKKD
                     TTQALELAKSALNLSEQINYLHGLGDAYTRLGLLDKNAGRLNEAVSNYKKSLHYRSAD
                     HDSIGIAKCYTNLGNVYNEKEMIDSAIFYQLSALRINEKVAEKGVVVDNYNNLGLSYK
                     NNGDYALAEEYLLSGYQVAKKTNNIEEQRLARICNNLANVYYEQGANMAKAKPYLLET
                     ISLADSVSDDLLADASNTLGLILMEQNPLPKDSILFYFQFAYNIYKSTGSTELAAVTS
                     NLGDFYVQTGNDELAIRYLLESNKICTDQELKTQLITNYKLLATVYINQGRTADAYEA
                     VSQALALNEEVYTLEKTEKIAQLQLAYETEKKDRIIAEEKATSAMQAGVIARQRFVGS
                     LLAGGTLIIIGAILFYMRQRQLKRKLEYEKKLADDRLRISADLHDEIGSALSSIGLYS
                     EVLKNTLQASDYPAAINYGSEIANTSRELMDNMSDLVWAVNPRNDSFEQLTTRLSNFG
                     YKIFGAGNTNFKVEITGDISSKEVPMAVRHQIYMILKEALNNSAKYAQAKNVLLSIKY
                     KPDSINLSVTDDGRGFDIANAGNGNGLQNMRRRAENIDAQFDICSGNTGTQLTFNIPI
                     P"
     gene            complement(8932..9624)
                     /locus_tag="PLL28_04255"
     CDS             complement(8932..9624)
                     /locus_tag="PLL28_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68563.1"
                     /translation="MALTFPTNMLANKPYVYLDESGTPLKIEVYVKGPQTGAAYALQA
                     SGAPQNSNTYLLTLTLTTVTTGSQVVKTIIDCSTLNGFSSTNFSKIVVRVVYQNNMVG
                     RTTIKIGANDFDAVDYKLFSYLQFIRTGTLKYHGLLELGSDELSQVNISQPSNPVGNQ
                     SDFIMRLNVNVVQANIYTNAVTPHFTYEIGSADGYDPTTYETLELRVDGSNPKKTKGS
                     TTDSEADASGDD"
     gene            complement(9628..10980)
                     /locus_tag="PLL28_04260"
     CDS             complement(9628..10980)
                     /locus_tag="PLL28_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNF68564.1"
                     /translation="MAIKIVNDSNLKMHTRYTTEQGTGMVPLLKPINFLGPKPTGTKI
                     DVPVEVADLAVHNATKIFNVTGTGLDGEGSVLAVLDTGIYAEHPDFGARVILSMNYKG
                     EKLPTSDIHDLHGHGTHVAGLAAAGRSKALAKFSGIAPKAKIVSVKVTEGNEDTTTWK
                     RIADGLDQVLNYNADAGTKPYNKVSCVLICFNSFDNFNNANEFDTTNNRLMKLFKICC
                     ESNIVVVVSSGNHFIDFADVSSRTITDGFGKKHKTPQVLEHTGLAFPAVSQHIICAGA
                     TANDDGIYYVSRSDLGDTVSFQKDDMAIFTQRIRKETNDTSNLSLFVAVPGLQMVSLD
                     NRPIQHAVLPGQYPQGYTVMSGTSQAAGVLAGMVLLLQQKIRNKLHIHESCVPIQKIF
                     TALKSTGEERNFDGFGHTVQPPTSTTHAAIGGLTGAHSPFRNFRIFDLIKAINYIQNH
                     "
     gene            11113..12483
                     /locus_tag="PLL28_04265"
     CDS             11113..12483
                     /locus_tag="PLL28_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNF68565.1"
                     /translation="MQVILTHGYSIIDDVREQQIMKPYAPLGILYISGWLDAHGINNH
                     VIDTTFITQDEQWNQIKEAKPEFLGVYVNLMTRKNVVQLIKKIKQEINLKHTKIILGG
                     PEVRNNAQQWLQTGADVLVVGEGEITFETLVTSWKTHKDLYQIAGIYFLDESGNMHFT
                     GEREKIKDIDILPFPARQHISIQKYLEVWKSRHGKNAISVSTMRGCPYTCKWCSRAVY
                     GMSYRRRSPQKVVEELQHIQEVYNPDTIWFVDDVFTISHKWLEEFARCVKEANLTLPY
                     ECISRADRMNAEVIHLLKESGCFRVWIGAESGSQRIIDAMDRRVKVESVQEMIRLSNA
                     NGIETGTFIMLGYPGETELDIVQTVKHLKAALPDLFTITTAYPIRGTELFAEIQNDII
                     NEPDWANSSDRDVTFKRNYPESYYKHAVRYVVNETNYAKSKAPLLKLKSMLARMMMWY
                     TRKFAV"
     gene            12480..13517
                     /locus_tag="PLL28_04270"
     CDS             12480..13517
                     /locus_tag="PLL28_04270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNF68566.1"
                     /translation="MTKLILVSPGFPADENDSAAVPYLQDYCLHLLKKVPAENINIVS
                     TQYPYTPKPYLWNGIQVYPIGGKNRKGIYQLINHIKTKKRLKSIVGKEPAVIHAFWLS
                     DAAMSASSFAAKRKLPCLVTLMGQDAKAGNPYFKLVSKHVAFTSLCNFQANVFRNDYQ
                     TVVKAVIPLPLPSIDLRPVISRSIDLLFVGSYIQVKQPLQFIEIVAEIKKKHSGVRAV
                     MIGGGPLLAEINSRVVALHLQDHIVVLESINRDEVFEYMQRSKILVHTSSFEGQCNVY
                     GEALLCGMHVLSYNVGYIADSPKHIVCADKNEFVLKIMQLLLHPINFTPVRSIDDEQC
                     INAYLNLYGMS"
     gene            13514..15031
                     /locus_tag="PLL28_04275"
     CDS             13514..15031
                     /locus_tag="PLL28_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNF68567.1"
                     /translation="MTKEEAQFGILLFNPRAAKHKARIPNSILSIAAAIAGKYPYIIV
                     DGNREDDPLSIIMQHLQTGKFNVFACTVMPGPQLKQAIPITKQIKSLFPDIYIVWGGY
                     FASNQSGVVMQSGWVDSIVYGPGDRAFPMLIDALEQDGPLDMIPNLIWKNGGEVHKNH
                     KDELYDQDTLPALPYQALHDFYDMRGYLGKTILGNKTIAYHSSIGCPFTCAFCGIVPI
                     YNARWKGRSAELIYKDIQFLKNTYGGDAIEFHDNNFFVSEKRTADFSTLMLNENMQWW
                     GEGRIDTIHKYSDDTLQLMRDAGCKMIFFGAETGNDAVLQLMDKGGTQTADQIRAFAA
                     RLKKFDIIPEYSFVLGTPGKDEAETLQRIEEDIAFIREIKSINPQTEIIIYVYSPVPT
                     EGSELFSAVQAGGFHFPQQLEDWISPAWESFDLRKNPLTPWLKPYMIDKIRSFEVVLN
                     GAYPTVSDIKLKSFDKKLMRSLASWRYKTEFYKFPIEVKLMQKLLRYRQPEIEGF"
     gene            15028..15693
                     /locus_tag="PLL28_04280"
     CDS             15028..15693
                     /locus_tag="PLL28_04280"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNF68568.1"
                     /translation="MKRKLMKFFTAWFVQPYVRRKMQRSSFYKWEDVTLFLPPGVFHP
                     GYFYSTKYLLQFILKQHISGKQLLELGAGNGLISIASAKRGAKVSASDISKEALRSLR
                     DNARTNHVGLTIIESDLFEQIPDAPYDYIVINPPFYPLDPRNDLERAWYCGQDYAYFR
                     KMFEQLPVFMHPQTKVYMILSEDTGIGQIENLAGFGNLRLEMTEKKQIFYEWNYIYEI
                     VRA"
     gene            15690..16649
                     /locus_tag="PLL28_04285"
     CDS             15690..16649
                     /locus_tag="PLL28_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68569.1"
                     /translation="MNSNLHIVKEEQFENASTLERAILRALIYFDIFQYPLSKQEIIL
                     FCNIVPENLKSVERVLDRLCNRLLIFKFDDLYSIHNSIGLADRRRCGNKGAIEAMPKA
                     IKRAKLIQAFPFVRSVNISGSMSKNYFDADTDVDFFVITSKNRIWLTRLMLTFFKKVF
                     LLNQRKYFCVNYFIDTEQFLIPDKNLFAATEIVTLKNVTGAKYYHQFLQENAWVSDYF
                     PNFEMPILITQNSRMPVFKRSMEKLLSGKFGDRMDDICFSLMQFFLRRKYRYLNETQF
                     EVNMRGNKHASKHHPQGFQFKVLEAFNKGCHQFSQKHDFPLDA"
     gene            16646..18124
                     /locus_tag="PLL28_04290"
     CDS             16646..18124
                     /locus_tag="PLL28_04290"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNF68570.1"
                     /translation="MKPVVICNTYFLHLDPKQERRMEPYPPLGTLYAAAVLREKGYDI
                     HFFDTIFCSGPEAIFNTLSKFQAGIFIICDDGFNYLTKMCLVNMREACSAMIKKAKEE
                     GFTILVSSSDATDHWQDYLNVGADAIVIGEPEQTIAACVDALQHENPALNTIEGLGLR
                     TTTGFAKTPARALMQDLDNLPLPAWDLIDMDQYRSRWMAHSGYFSINMTTTRGCPFKC
                     NWCAKPIYGNRYHSRSAKAVADEINLLRNTYQVEHIWFCDDIFGLKPNWINSFAGLMQ
                     EQPIPFKIQCRADLLLDENNIQSLAGAGCDEVWIGAESGSQKILDAMDKGTTIAQITE
                     SSRLMYRSGIKPCFFLQFGYPGETLTDIKSTMQLLRNCKPYDIGISISYPLPGTVFYD
                     RVFAQLSNKQNWNDSDELLMMYHGTYSAAFYKYLHRYVHRRFRMLQGMKCIREIFSGK
                     RNTTSPPLKRLFALPIHSVLCLYYFLMVQTKKQPVSELQVAS"
     gene            complement(18142..20976)
                     /locus_tag="PLL28_04295"
     CDS             complement(18142..20976)
                     /locus_tag="PLL28_04295"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HNF68571.1"
                     /translation="MTRIVIRQLLLVMIFGVTALSVNAQITYTTGWTADSLAAYLAGA
                     GVEIDNATINCHNLAIGKFDCVDCNVGIDSGVVLTTGRVSNILGPNNSGSTGTNNGWP
                     GDPDLNAYPGVGTTHDACALEFDVYAPGDSLKFDYVFGSEEYPEYVGSINDAFVFFIS
                     GPGYPTLTNIALIPGTTLPVTIDNVNATSYPMYYIPNGTGGTGIYATSPYYIEYDGFT
                     TVLTAEAEVTPCEWYHLKLVIGDESDFVWDSGVFLKAKSLVTNVLADFTYPGVPFGGT
                     AEFCTTGTDPAPLYATGATAGTFTCTPSGLVFDAATGAIDVSASDPGSYTLTNTLLTG
                     ICYGDTATWDITVNIYEPDAAGFSYPGSPFCANESDPSPIPMIGASLGTFTATPAGLV
                     INAATGTIDLSASTPGTYTITNTVASGTVCPDGVATNTVTIYPTYAVSQSGNICLGDP
                     FILPDGATVYTGGTYVTTLPTIHGCDSVITTTLAAHPVYNYTLNPAICSGATYTLPDG
                     TVVSTTGTYINNFTSVWGCDSNYTINLTVSPVPVVNMNVHICSGETYILPDGTAVGVS
                     GVYPVTLTTVAGCDSTINTTLFVHPVYAITQNPVICDDVTFTLPDGSVVNTTGTYVTN
                     LLTTKGCDSIITTNLTVNPTYDIIMNPEICIGDTYTMPDGSVTGTGGTTVFPYASING
                     CDSIITVNLTVNPLPVITWPIDDIFCLEQNSIALEATPPGGIYSGPGTSGDHFLTNDA
                     GVGGPYTLTYEYTDANGCYSSMTVTTSVDDNFVTAWGDTTLYYGSSAILHSESGGDYT
                     WSPTNGVECPLCPESYVLPPYSIDYIMTSVDENGCVASDNVMITVLPLPPNDFFVPNT
                     FTPNGDNINDIFYIFGYNVLSITDFEIFDRWGKVVYRGENLDANDINLGWDGTINGDP
                     ANNGVYAYYITVQFDNGQQMKRAGNITLIR"
     gene            21067..21534
                     /locus_tag="PLL28_04300"
     CDS             21067..21534
                     /locus_tag="PLL28_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNF68572.1"
                     /translation="MPQTRTVVAFYAVIASMLTFASCSNSRSAMKNVIYTPNAPAPIG
                     PYSQATEAGGFVFISGQIAINPANGEMYTGGDIAVETRQVMENLKAIVEQSGSTMGNV
                     LKCTIFLRDMNDFAAVNAVYGEYFQEYPPARETVAVVGLPKNARVEISAMVSR"
     gene            complement(21908..>23595)
                     /locus_tag="PLL28_04305"
     CDS             complement(21908..>23595)
                     /locus_tag="PLL28_04305"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68573.1"
                     /translation="QNTIGGNNYDYLECVIQTADGGYFVGGKSFSNISGDKTENSNGS
                     QDYWVIKLNNLGNIEWQNTIGGSGTDVLQSVAQLPDGTYYVGGYSFSGASGDKSENNI
                     GDNDLWLLKLNSLGVVEWENTIGGNLDDKLRSVNVTSDGGCIIGAQSTSSISGDKTED
                     SIGYSDYWIIKLNSFGIIEWQNTIGGSEAENIASVIQLSDGQYFVAGYSQSNISGDKT
                     ENVYVAHDYDYWVLKINDIGAIQWQYNIGASYWDKLSCSIQTMDGGFFLGGYSQSNIS
                     GDKSENSFGGTWDYWVVKLAGPACTPAPEMCNLIDDDCDGLIDEGASPAISISAGGAT
                     TFCQGSNVILTATHTGTGLQWKKNGTTIPGATGVTYAATKTGIYTCESSNACGATLSA
                     SISVTANKNPPASITAGGPTSFCAGGSVVLTANAGGGLSYQWYKGATPIAGATNIAYT
                     ASTAGNYKCRVTKTATGCFKNSNAIMVSIVCRGGENETPSQILYAYPNPATTQLNITC
                     SANINTAQLYNGLGQLIMEISVSDGYAEADISTLTAGMYLIRAGGEVAQWVKQ"
BASE COUNT         7119 a         4791 c         4857 g         6828 t
ORIGIN      
        1 ccggatactt tttcttttac ggtaaagttt acggtgaaag tttcggcgga agttgattca
       61 tttttaattt ttgcagacaa acctgaaggg ttgccataag gtataggtgt gtttgcaagg
      121 caatatactg cttgcacatt tgccttatca gacaatggat ttcctaagcg agtttcaggg
      181 cggaaacctg acaatgtcat tggcgtgcca taagcagttt ggctctgata agtacctagt
      241 gaattagcta cagtattagt acataatacc gtaggcgcac ctaaaggcaa tgcaagtgca
      301 gtagtagttt gatattcaaa ggctacatat aaaccttgtc cggctactgt attaaataca
      361 gaagttccgg taccaccaac aggaacatct atactaaatt gaactccgga gttcgtaata
      421 gtgattgttc catcaataac tttagtcata cccacagtgg agaaggtagt tcctttgcta
      481 taggcaagat ctgccgagtt ttgcaaatac accttcaagc tacctgttgt agtaacattc
      541 tgaggcgaac cggcctgcca ggtccatcca acagttgtaa cctgatcaga gccataaccc
      601 gaagtagtca tttcggaagg cgagatataa tataatgtgt taatgtaaag gcgcgatcct
      661 tgaggtgcgc gaccattacc ggatgtagat ccgtttcccg gcaaaaccgt aattgtggca
      721 gcgtaagatg catgcgccac acatagcatt agcagggaaa agatgacgtg tttaaacgtt
      781 cttttcataa taattggatt ttgggtgaag gaaaaaattg ttgaaaagac caccgacagg
      841 tagcccatta gaatgtaaaa tgatgctcgt acgttgtgac atttgtgtgt gtttcaagta
      901 ccgggatcag ggcttgctgg gggcagagac atgatcaacc gaaatgaaaa ttaggagtaa
      961 aattcaaatt ttcaaatttt tcacaaatat tttttcagga ggtagtgata cctgcctagg
     1021 ctgattcggc aataacctgc tacctttggg gcatggaaac agccttattc catcagaaca
     1081 aactcatggt gtacaacact ttgagccgtg aaaaagaaca ttttattccg ctacatgcgc
     1141 catttgtagg catgtatgcc tgcgggccta ccgtttatag tgaaccgcat cttggaaatt
     1201 gccgaagctt tgtgatgtac gacctgatgg taaggtatct tacccatttg ggctttaaag
     1261 tgcgctattt gaggaatatt accgatgctg ggcatgttga aagcaccagt ggagaaaccg
     1321 gcgacaggat aggaaaacaa gctcgattgg aacaagtgga gccgatggag atcgtgcaga
     1381 aatacacggt gaaatttcat tggattatgg atctattgaa ttgtttacct cccagcatag
     1441 agccaactgc aacaggtcat attgttgaac aaattgaaat gacgaaaaca atcctggcaa
     1501 atggatatgc ttatgaaacg gatgaagcca tttattttga tgtcgaaaaa ttttcgaaag
     1561 attatccata tacacaactc agcggacgca accttgaaga actgctgaat aacacgcgtg
     1621 aactcggcgg acaatcaagt aaaaaaggac gattggattt tgcgcttatg atcaaagcta
     1681 aaccagaaca tattatgcgc tggccttcgc cgtggggtga tgtatatccc ggttggcata
     1741 ttgaatgttc tgccatgagt acgaaatatc tgggcaaaac atttgacatt cattgcggtg
     1801 gcatggattt aattcctaca catcatacca atgaaattgc gcagagcatt gcatgcaatc
     1861 ataccactcc tgtaaagtat tggctgcatc cgaatatgct cactgtcaac ggacaaaaaa
     1921 tgagtaagag tttgggcaat gcatttttgc cggtagaatt atttgaaggc acgcatccat
     1981 tgcttgataa aggatattca ccaatgacgg tgcgtttttc gatgttacag gcacattatc
     2041 gcagcacact tgattttagt aatgatgcat tgaaggctgc tgaaaaagga tacaagcgct
     2101 tgatgtcggc aatgaattta ttaaaaggca ttacagtatc cgatactgtc aatgccgatg
     2161 aagaagccgc cgtaaataaa atattgcttg gtgtttacga gggtatgaat aatgatttta
     2221 atgcggctca ggcaattgca gaattgaatg aagcggcaac tatcatcaac acctggaacg
     2281 ataaacaaaa taaaaaagca tcgatacagg catttacgtt aagcagactg caacaaacct
     2341 atcgcgcatt tcttattgac atttttggat tacaagagga gacagaaaat gacaatagca
     2401 aattaagtgg tgtaatggac cttgtaatcg acattcgcaa agatgcacgt ggaaagaaag
     2461 attttgctac cagcgacaaa attcgcgatg cgctgcaatc tgtgggaatt caactgaagg
     2521 atagtaagga tgggacaacg tgggagggga attaatttcc tctaatgatg atttcttcga
     2581 atattcacat taattaaaca cgttatttcc accatttaca taagctatct tccatttagt
     2641 cttgctattc aggtaaatgt ttgaatttat gtaggtttgc cttatgtcgc aaacaccttt
     2701 tgacttagca gctcccgaat acgacagccg tttcacagaa actcccgcag gcaaggctca
     2761 gcgagatatg gtgtggcgat ttttgcataa acagcttcca ccttctccgg ccaacattct
     2821 cgaaatcaat tgcggcacag gcgaagatgc cgtgcacctg gcaacagcgg gatttcatgt
     2881 tactgcaaca gatgcatcag aaaatatgct tgccgaagcc ggcaagaagt ttgcatcagt
     2941 acgaatgaac gttcattcat atgtatggga tgtcagaact ccatttattg gaactgaaaa
     3001 gtttaatgtg atattttcaa attttggcgg attgaattgt ttatctcctt ccgagttgcg
     3061 cagtttattt aaaaggatgc aatcgcaatt atctgaaaat gggaaattaa ttattgtcgt
     3121 gatgggtaga aattgcccat gggagcgatt gcttttttca ttgaaaggga atttcaaaca
     3181 agcaaagcgc agaaaatcaa aaaatgccgt aatagccaaa acaggaggtg aaaactcatt
     3241 acctgtatgg tattacagca tagaggagat agttgaattt gcgggcgata attttaatct
     3301 ggttttctcc agaccaattg gattatgtat cccgcctgta tatggcaaaa aagctttctg
     3361 gcataataca atgttgattg cgcttttgag aaatattgaa actgcaatcg gagggctaaa
     3421 actatttcgc aattcaggtg atcatttgtt cattacgcta caaaaaaatg aagccgatca
     3481 ataagaccgg cttctaaaga gaacacactt agaacgaaga gatggtaata gtgccatcgc
     3541 cgatgacatc tttatcggaa ttaacaactt ggtcgggtaa catcaatcta gctgcagcat
     3601 caaatggtgc acttaatacc aggttttccg attcactaat gcaggtgcca aatgtttttc
     3661 cttcacgcca tttcaataag aatggtagta attccacagc accgttggca ttgcaggctt
     3721 tggatccgct agcgcaatta aatcctgccg atacccaatt gtctgcttga gaagcaccat
     3781 aacatgcagt ggagtatacc atgcgaagtt tggatgccgg aagatttgca tcccgaatgg
     3841 catttacaat cgtcgtcatg tgaacagtat cattgctcaa agtcagcgag tcgcgaccac
     3901 catggaggaa tacgatcaaa tccacttccc atatattgct attacccgtc atacgcgaaa
     3961 gatttgatac aatgcgatca agtgttacat ctccgggtcc aagcacttta atttcatggt
     4021 aataaccacg caaattggca tttacaagat ttacaccatt tgactcagaa aactggtata
     4081 atggtctgaa atttttaagt gttccggtaa gcacctgcga aaggtcagcc aatacaagta
     4141 atgctctgtt ttcagatctg atggcgggtt ttgtaaaagg taataggata ggaacgctga
     4201 tagcagcatt tacatagatg aaaggacctg tatggcgtcg gccaattgaa tcggttacct
     4261 cgaaagtgag attcatgaaa tcgtgttccc atggccaaaa tccatccggt tcatctttcc
     4321 aatcaatggt tcttccaaca aattttccac gcgagtcggt ggtagcggtc agaattacat
     4381 tatctgacga gcgatcatcc tgatcgataa tacgaactgt agcatttttt gcgggtgttg
     4441 tatcaagata agatactgag ccgctaatgt ttaaacgtga catgttttta gttttattgg
     4501 tgataaaatt ttcagggaat agcagtagtg ttcggattac ttcaaactat tctattgaca
     4561 atgcaattat tatttgtcgg ccgagtcaat aatgaattgt aaaatcaaca atgtaaagca
     4621 atcaagaaat tacagccgct acaatagccc aaacgggtga aattaaaatc ccgggtcatt
     4681 aaaacccggg atacacttta tacgaagaga acaattcgat aagccgtata caatcaagct
     4741 gaatgcgata tcaatttatt attcattatc agagatagac acgcctaccc acgaaattcc
     4801 actccagatt aatgtaacca catcatcatt cccgataata aacgtttgcg gtatattgat
     4861 attcgacgca tcaccatccg ataattcaag gaaggtgtcc gacatcatga aaccttgtaa
     4921 aataattatc tgccccggtg tgataccatc acccaaaata attgttctgt tagcagctaa
     4981 cgtgtcatca gactgcaact gcaaatagct tctatcacct acttccaaaa aataattatc
     5041 ataggtgatt actattacat cgggttcagg gtatagggag atagccccat tcaccgaaag
     5101 ctttgtcatg ggagcagtag ttccaatacc cacattggtt tcaggggaaa tagtatatat
     5161 catttcgccg cttccggccc atttattatc cttgctggtt tgcgcagaaa cagcagtttg
     5221 agcataatgg gcataaggca cagaattcaa tgcttcggta gtgatgatat cataaatatc
     5281 atcgccatca atgtcgcagc ggatgagtat ctccatataa tcagatcccc atttgataga
     5341 atcaaataca ccggagatca ccaagccatt gccaatcttc agcgaaagtt gtccattcat
     5401 atctgtataa acagcatgac gttcagcata tacctgactg ccaccgacca agatcccgaa
     5461 ctcgaattta atttctgtat ttattacaag ttcgttggaa gggtttctga taactgcctg
     5521 ataatttaat gcatcttgcg agcttcccca taatgggatc aataaaaata ttgcaagaaa
     5581 tagctttgat gttttcatat ttatttattt taggatagtg aatatctgtt ttgccgttac
     5641 aaatcccttc ggactttgga tagctaccaa gtattgtccc gcagacaaag aagtaacatc
     5701 caattgggtg ctgtactcat gctcaaattt gaattgcatt acttcggcac ccattaaatt
     5761 ataaattgtt ccttccgcac ccgaaatgag catcgcatca cattcaattg taatcagcgc
     5821 agtagccgga ttggggtagc aggatataac tatttcagga gatagcccgg tcacattgtc
     5881 tgcttcaaaa attgttgtgt ccaaagctgg ttcgggaatg gtggtgacac gctgaaatcc
     5941 ctcttcaagc gtatatccat atttatcgaa agtgcgtata caagtctcgc caatggtata
     6001 tgaaatattg ccgtgagccg cgaaggtttg accatctgtt gcaattacaa atgaactaat
     6061 ggtttgccca tcaacatttg taatcaaata gcaatagcaa acaataacat aaaaaattcc
     6121 tttcatagca ttcggggatt aattgggtga atatgtaact ggtctgtaac aatgcaaaga
     6181 tgactagcca aagctgtttc ggcaatcacc tatatgggtg aaaatatttg accgataaaa
     6241 aaaccccatg accgaattgc catggggttt aatgatttat gacataaata aaactaactg
     6301 atcaaatttt cctgaagtgc ttttgccact gcttctgttt ttgatgccac ctgaagttta
     6361 tcatagatct tttttacgtg gctatgaaca gtttcggcag aaataaataa tgcttctcca
     6421 attaatttat aactcttacc ttttgcgagc agttgcaata cttcaaactc gcgatcgctg
     6481 agctgatagt ccttttcagc cattggtttt tccatagcag cagacctgaa taattcgaca
     6541 acacgtcttg ctatacttgg agtcataggt gcgccgcctt catacacttc ctttatggct
     6601 tccagcacct gatgcggagg ggtgtttttc aacagatagc cattggcacc atagcagatg
     6661 gaatagaata tttttctgtc ctcatcaaaa cttgtctgca taatgattct tacatccgga
     6721 aacttcgcat gcagcatttt cactgcatcg attcccgaca ggccaggcat ttcaatatcc
     6781 atcagcacaa catccgggtc agctttctct acgtctttga ccacattttc cgcatttgga
     6841 aatccgcctc cccattcgaa gccgggtgtt cctttcagca gggcactcat agcgtcacgc
     6901 actctgcgat ggtcatcaaa aacgcatact ttgatagaca tgtgatgtag tttttacaaa
     6961 gatgaatgaa tttgaatcaa tggtcaataa cccggtcggg ggatttcaag gtattggaat
     7021 attgaatgtc aattgagttc cggtattccc ggagcagatg tcgaactgcg cgtctatatt
     7081 ttctgctcgt cggcgcatat tttgaagtcc gttgccattt cctgcattgg caatatcaaa
     7141 tccgcgaccg tcatcagtga cactcaaatt gatactatca ggtttgtact tgatcgacaa
     7201 tagcacattc ttcgcctgag catattttgc actgttattt aaagcctcct tgaggatcat
     7261 gtaaatttga tgtcttacag ccataggcac ttctttggaa gaaatatcgc cggtaatctc
     7321 gactttaaaa ttggtgtttc ctgcgccaaa tattttatat ccgaaattcg acaagcgcgt
     7381 ggtaagttgt tcaaaactat cgttgcgcgg gtttacggcc cataccaggt cgctcatatt
     7441 atccatcaac tctcgtgaag tattagctat ttcgctgcca taattaattg ctgccggata
     7501 gtcagatgcc tgcagggtgt ttttcaatac ttcactatat aaaccaatac tactcaaagc
     7561 agaacctatt tcatcatgca aatcggcgga tatgcgcagc ctgtcgtctg ctaatttctt
     7621 ttcatactcc agctttcttt tgagttgccg ctgacgcata taaaacaata tggcaccaat
     7681 aattatcagc gtacctcctg ctaacaggct acctacaaag cgttgtcggg caataacacc
     7741 tgcctgcatg gcggatgttg ctttttcttc tgcaataatc cggtcctttt tctcagtttc
     7801 gtaggcaagt tgtaactgcg caattttttc tgttttttca agtgtataca cttcttcatt
     7861 caatgccaac gcctgagaaa cggcttcata ggcatctgca gttcttccct gattgatata
     7921 aaccgttgcc agtaatttat aatttgtaat caattgtgtt ttcaattcct gatcagtaca
     7981 aattttattg ctctccagca ggtatcttat cgctaattca tcattaccgg tttgcacata
     8041 aaaatcacca agattgctgg ttacggctgc gagttctgtg cttcctgtgg atttatagat
     8101 attgtaagca aattgaaaat aaaataaaat actatctttt ggaagggggt tttgctccat
     8161 caatatcagg ccgagtgtat tggatgcatc agccagcaaa tcgtcagaaa cactatcagc
     8221 caaagagatg gtttccagta aatatggttt ggcttttgcc atattcgcac cttgctcata
     8281 atatacatta gcaagattat tgcaaatacg tgccagtcgc tgttcttcta tgttatttgt
     8341 tttttttgca acttgatatc ctgacagcag atattcttca gcaagcgcat agtcaccatt
     8401 atttttgtaa gacaatccaa gattattata attatctacc actacaccct tttcagcaac
     8461 tttttcatta atacgcaaag cggaaagttg ataaaaaatt gctgaatcaa tcatttcctt
     8521 ttcgttgtat acattgccaa gattagtata gcacttagct ataccaattg aatcatgatc
     8581 ggcactcctg tagtgaagac tttttttata gttagataca gcttcattca acctacccgc
     8641 atttttatcc aacagtccca atcgcgtata ggcatcgcct aggccatgca ggtaattaat
     8701 ttgctctgac agattcaatg ctgatttagc tagctcgagg gcctgagtgg tatccttttt
     8761 aataagatcg aaggacattt tgttaagcgc attgatcctg gctgtgtcgt taccggtgcc
     8821 tgcattcatc aggaaggaat aactaactag cagcaagaac aaataaaaag cccctgatct
     8881 catgtaggtg tgtttactgc taaggtaagc aacaacagag acttatcggt tttagtcatc
     8941 accagaagca tctgcctcgc tatcggtggt tgagcccttt gtctttttag ggtttgaacc
     9001 atcaacacgc aactcaagtg tctcataagt tgttggatca tagccatcgg cacttccaat
     9061 ctcataagta aaatgcgggg taacggcatt tgtgtaaata ttagcctgaa caacatttac
     9121 atttagtcgc ataataaaat cagattgatt accaacggga tttgaaggtt gagagatatt
     9181 cacttgactc aattcatcag aaccaagttc caataaacca tggtatttaa gtgtaccagt
     9241 tctgataaac tgtaagtatg aaaacaattt atagtcgaca gcatcaaaat catttgcacc
     9301 aattttaata gttgttcttc caaccatatt gttctgataa accacacgca caacaatttt
     9361 actaaagttc gtactactaa atccattcaa agttgaacag tcaattattg ttttaacaac
     9421 ttgcgatcct gtagtaacag ttgtaagtgt gagggtaagc aaataagtat tggaattttg
     9481 tggagcaccg cttgcctgca atgcatatgc agcaccggtt tgaggacctt taacatacac
     9541 ctctattttc aaaggtgttc cgctctcatc taaataaacg tacggcttgt ttgcgagcat
     9601 gttagtggga aaggtaagtg ccattgatca atgattttga atgtaattaa ttgccttaat
     9661 aagatcgaaa atgcggaaat tcctaaacgg tgagtgagca cccgtgaggc ctccaattgc
     9721 tgcatgcgtt gtagatgttg gtggttgaac ggtatgtccg aagccatcga aatttctttc
     9781 ttcgccggtg cttttcaatg cggtaaatat tttttgaatg gggacacaag attcatgtat
     9841 atgcaactta tttctgattt tctgctgcaa tagcaatacc attcccgcta agacaccggc
     9901 agcctggctt gtacccgaca taacggtgta tccttgagga tattgacctg gtaggacagc
     9961 gtgctgaatc ggccgattgt ctaatgatac catttgcaat ccgggcactg caacaaataa
    10021 ggaaaggtta gatgtatcat ttgtttcttt gcggatacgt tgtgtaaaaa tagccatatc
    10081 gtctttttga aaagaaacgg tatcgcctaa atccgatctg cttacgtagt aaataccatc
    10141 atcattcgcg gttgcgcctg cacaaataat atgctgactg acggcaggaa aagcaagtcc
    10201 ggtatgctca agaacttgtg gtgttttatg ttttttaccg aagccatctg taattgttct
    10261 tgaagagaca tcagcaaaat caataaaatg gttgccactc gacaccacca cgacaatatt
    10321 actttcacaa cagattttaa aaagcttcat caacctgtta tttgttgtat caaattcatt
    10381 tgcgttgttg aaattatcaa agctattaaa gcaaatgagc acacaactaa ccttattata
    10441 aggtttagta ccggcatcag cattgtagtt caacacttga tcaaggccat cagctattcg
    10501 tttccaggtg gtggtgtctt catttccttc ggtaactttt acagatacaa tttttgcttt
    10561 tggtgcaatt ccggaaaatt tagcgagagc tttacttcta ccggcagctg ccaaaccggc
    10621 tacatgtgta ccatgcccgt gtaaatcatg aatatctgat gttggcaatt tctcgccctt
    10681 gtagttcatg gataaaataa ctctggctcc aaaatcagga tgttcggcat atattcctgt
    10741 atccaaaacc gccaacacac tgccttcacc atctaatccg gtgccggtta cattaaatat
    10801 tttggttgca ttatgcactg ccaggtccgc aacttctacg ggtacatcaa tttttgtacc
    10861 ggtaggtttt ggcccaagaa aatttatggg tttaagcaga ggcaccatac ctgttccttg
    10921 ttcggtagta taccgtgtgt gcatttttaa gttactgtca ttgacaatct tgatagccat
    10981 gtagtttcga tttacaagtc aaaagtagaa taatacctta aaatacacaa tcccccgacc
    11041 gggtgaacct tactgcattg ataaaccttg tcagccttgg gttgactgtg aataattgaa
    11101 tagctttgtt gcatgcaggt aatactcact catgggtatt caattattga tgatgtgcga
    11161 gagcagcaga tcatgaagcc ttatgcacca ttaggaattc tttacatttc cggttggctg
    11221 gatgcacatg gcattaacaa tcatgtgata gacaccacat tcattacaca agacgagcaa
    11281 tggaatcaaa taaaagaagc aaaacctgaa ttccttggtg tttatgtgaa tttgatgacc
    11341 aggaaaaatg ttgttcaact catcaaaaaa ataaagcaag agataaatct caagcacact
    11401 aaaattattc ttggcggtcc ggaagtgcga aataatgcac aacaatggtt gcaaacggga
    11461 gcagatgtat tagttgttgg cgaaggggag atcacttttg aaacattagt gacaagctgg
    11521 aaaacacata aagatcttta tcaaattgcc ggcatatatt ttttggatga gagcggaaat
    11581 atgcatttca ccggtgaacg tgaaaagata aaagatattg acattttacc tttccctgca
    11641 cgtcagcata tctccattca aaaatatctg gaggtatgga aatcgcgtca cggcaaaaac
    11701 gccatttcag tttctacaat gcgtggctgc ccatatactt gtaagtggtg tagtcgcgca
    11761 gtatatggca tgagttaccg ccggcgttca ccacaaaaag ttgtagagga gttgcaacac
    11821 attcaagaag tttataatcc cgatactatt tggtttgtcg atgatgtgtt tacgatcagt
    11881 cataaatggt tagaagaatt tgcacgttgc gttaaagaag caaatttgac actgccatac
    11941 gaatgtattt cccgggcaga tagaatgaat gctgaagtaa ttcatttact gaaagaaagc
    12001 ggatgtttca gagtatggat tggcgctgaa agtggctcgc agcgtatcat tgatgcgatg
    12061 gaccgaaggg tgaaagtgga aagtgtgcag gaaatgatca gactatccaa tgcaaatggt
    12121 atagaaacag gcacctttat tatgttgggc tatccgggcg aaacagaatt ggacattgtc
    12181 caaaccgtga aacatctgaa ggctgcatta ccggatttat ttaccattac aactgcatat
    12241 cccatcagag gaacagagtt atttgcagaa atacaaaacg acatcatcaa tgaaccggac
    12301 tgggctaatt caagcgacag ggatgtcact ttcaagcgca attatccgga atcctactat
    12361 aaacatgctg taagatacgt agtgaatgaa acaaactatg caaaaagcaa ggcaccattg
    12421 ttaaagttga aatccatgtt agcgcgaatg atgatgtggt ataccagaaa attcgctgta
    12481 tgacgaagct gatacttgta agcccgggat ttcctgccga tgaaaacgac agcgctgcgg
    12541 tgccatatct gcaagattat tgccttcact tattaaaaaa ggtgcccgca gaaaatatta
    12601 atatcgttag cacacaatat ccatatactc caaaaccata tttatggaat gggattcaag
    12661 tctaccccat aggcgggaaa aacagaaaag gcatttacca acttattaat catattaaaa
    12721 ccaaaaagcg tttaaaaagt attgttggca aggagcctgc cgtcatccat gcattttggt
    12781 tgagcgatgc agcaatgagt gcttcttcgt tcgcagctaa acgtaaatta ccatgccttg
    12841 taacattgat ggggcaagat gcaaaagcag gtaatcctta ttttaaatta gtatcaaaac
    12901 atgttgcatt tacatcttta tgcaattttc aggctaatgt ttttcgaaat gattatcaaa
    12961 ctgtagtaaa ggcagtaata ccgcttccat taccctcgat tgatttacgc cctgttattt
    13021 caaggtcaat agatttattg tttgtaggat catatattca agtaaaacag ccattacaat
    13081 ttattgaaat tgttgccgaa ataaagaaaa aacacagcgg tgtcagggct gtaatgattg
    13141 gaggaggtcc actcttagct gaaataaatt cacgcgttgt tgcattgcat ctgcaagatc
    13201 atattgttgt gctggaaagc ataaaccgag atgaggtatt cgaatacatg cagcgctcta
    13261 aaattttggt acatacatca agttttgaag gccaatgcaa tgtttacggg gaggcgctgc
    13321 tttgtggaat gcatgtgctc agctacaacg ttggttatat tgcagattca ccgaagcata
    13381 ttgtatgtgc ggataaaaac gaatttgtgt taaaaatcat gcaattactt ttgcatccta
    13441 ttaactttac tcccgttcgt tctattgatg atgaacaatg tattaatgca tatttgaacc
    13501 tgtacgggat gtcatgacca aggaagaagc acaattcggt atattattgt tcaatccgcg
    13561 ggctgcgaag cacaaggcca ggataccgaa ttctattcta tcaattgcgg ccgccattgc
    13621 cggaaaatat ccctatatca ttgtagatgg caatcgcgag gatgatcctc tttccataat
    13681 tatgcagcat ttgcaaaccg gaaaattcaa cgtatttgca tgcactgtaa tgcccgggcc
    13741 gcaactgaaa caagcaattc ccattacaaa acaaatcaag tcactttttc ccgacatata
    13801 tattgtatgg ggaggctatt ttgcaagcaa tcaatccggc gtagtgatgc agtcgggatg
    13861 ggtggatagt atagtgtatg gtccgggtga tcgtgcattt ccgatgctaa tagatgcgct
    13921 agaacaggat gggcctcttg acatgattcc aaatttgatt tggaaaaacg gtggtgaggt
    13981 tcataaaaat cacaaggatg aattatatga tcaagacacc ttaccggcat tgccgtatca
    14041 agcattacat gatttttacg acatgcgcgg ttatttggga aagacaattc ttggcaacaa
    14101 aacaatcgcc tatcatagca gcattggttg tcctttcacc tgtgctttct gtggtatagt
    14161 tcctatttac aatgcgcgtt ggaagggtag atcagcagaa ttaatttaca aggatattca
    14221 atttctgaaa aacacctacg gtggcgatgc tattgaattt cacgataata atttctttgt
    14281 atcagagaaa agaaccgctg atttctcaac actcatgctt aatgaaaaca tgcaatggtg
    14341 gggagagggt cgcattgata ccatacacaa atacagcgat gataccctac aattgatgcg
    14401 tgatgccggt tgcaaaatga tattctttgg cgcagagacc ggcaacgatg cagttttaca
    14461 attaatggac aaaggaggca cacaaacagc ggatcaaatc agagcctttg cagcgcgttt
    14521 gaagaaattt gatattattc cggaatattc atttgtgtta ggcaccccgg gcaaagacga
    14581 agcagaaacc ttgcagagga ttgaagaaga tatcgcattt attcgggaga taaaatctat
    14641 caatccgcaa actgagatta tcatatatgt gtattctccg gtaccgacag aaggcagtga
    14701 attattttca gccgtgcaag caggaggatt tcattttcca caacaactcg aagactggat
    14761 atcaccggca tgggaatcct tcgatttacg aaaaaatccg cttaccccgt ggcttaaacc
    14821 atatatgata gacaaaatca ggtcatttga agttgtgttg aatggcgcat atccaacggt
    14881 gagcgatatc aaattaaaat ccttcgacaa aaaactcatg cgttcacttg catcatggcg
    14941 atataaaaca gagttttaca aattcccgat agaagtaaag ctcatgcaaa aactactgcg
    15001 atatcgacaa cctgaaattg aaggattttg aagcgcaaat tgatgaaatt ttttacggcc
    15061 tggtttgttc aaccgtatgt gcggaggaaa atgcagcgtt cgtcatttta taaatgggag
    15121 gatgttacct tgtttttacc tccgggtgta tttcatcccg gttactttta tagtaccaaa
    15181 tatttacttc aattcatact taaacagcat atttcaggta aacaattact agagttaggt
    15241 gccggcaatg gcctcatcag cattgcatcg gcaaaaaggg gtgcaaaagt aagtgcaagt
    15301 gatattagta aagaagcctt gcggtcatta cgggataatg cacggacaaa tcatgttggg
    15361 ttaacaatca ttgaaagtga tttattcgaa cagattccgg atgctcccta tgattacatc
    15421 gtaatcaatc cgccatttta tccattagat ccacgtaacg acctggaacg cgcctggtat
    15481 tgcgggcaag actatgcata tttcagaaaa atgtttgagc aacttcctgt atttatgcac
    15541 ccacaaacaa aagtatatat gatattatct gaagataccg gtatcgggca gatagaaaat
    15601 ttagctggat ttggcaattt gcggctagaa atgacagaaa agaaacaaat attctacgaa
    15661 tggaactata tttatgaaat agttcgcgca tgaatagcaa cttgcatatc gtaaaggaag
    15721 aacaatttga aaacgcctca actcttgagc gtgctattct gcgtgccttg atctacttcg
    15781 atatatttca atacccatta tccaagcaag aaatcattct attctgcaac attgtccccg
    15841 agaatttaaa aagtgttgaa cgtgttctcg acagactatg caatcgattg ttaattttca
    15901 aatttgacga cctttattcc attcataatt caattggttt ggccgacaga aggcgatgtg
    15961 gaaacaaggg tgcgatagag gcaatgccca aagccatcaa acgagcaaag cttatacagg
    16021 cattcccgtt tgttcgcagc gtgaatatca gtggaagcat gtcgaaaaat tatttcgatg
    16081 ccgatactga cgtcgacttc tttgtaatca catctaaaaa caggatttgg ttaacaagac
    16141 tgatgctaac tttctttaag aaggtatttc tattgaatca acgcaaatat ttctgcgtta
    16201 actactttat tgacacagaa caatttctaa tacctgataa aaatttattt gccgctacag
    16261 aaatcgtaac attaaaaaat gtgacaggcg ctaaatacta ccatcaattt ttacaagaaa
    16321 acgcttgggt ttcagactac tttcctaatt tcgagatgcc gattttaatt acgcaaaatt
    16381 ctcgtatgcc tgtgtttaaa cgtagcatgg agaaactatt atcaggtaag ttcggtgacc
    16441 gcatggatga tatatgtttc agtttgatgc agttttttct gcgaagaaaa tatcgctatc
    16501 tgaacgaaac acaatttgag gtgaacatgc gtggaaacaa acacgcttca aagcaccacc
    16561 cccaaggatt tcaattcaaa gtattagagg ccttcaataa gggatgccat cagttttcac
    16621 aaaaacatga ttttccattg gatgcatgaa gcctgtagta atttgtaata cttacttttt
    16681 acacctggat ccgaaacagg agcggaggat ggagccatat ccgcctttag gcaccctata
    16741 tgctgctgcc gtgttgcgcg aaaaaggata tgatattcat ttctttgata ccattttttg
    16801 ttccggtccg gaggcaatct ttaatacatt gtccaaattt caagccggca tattcattat
    16861 ttgtgacgac ggatttaatt acctgacaaa aatgtgtctg gtcaacatgc gggaggcctg
    16921 ctcagcaatg atcaaaaagg ccaaagaaga ggggtttacc attttagttt cgagtagtga
    16981 cgccactgat cattggcaag attatttaaa tgttggagcc gatgccattg taatcggtga
    17041 acctgaacaa acaattgctg catgtgtaga cgccttacaa catgaaaatc cggctttaaa
    17101 taccattgag ggattaggat tgcgcacaac aaccggtttt gcaaaaacac ctgcacgcgc
    17161 attaatgcaa gacctggata atttgccgct tcctgcgtgg gatcttattg acatggatca
    17221 atatcgttca cgctggatgg cacattcagg atatttttcc attaatatga ccacaacacg
    17281 tggttgtccc tttaaatgta attggtgtgc caaaccgatt tacggcaatc gatatcattc
    17341 gcgaagcgct aaagctgttg cagatgaaat taatttactg cgaaatacct atcaggttga
    17401 acatatttgg ttctgcgatg acatttttgg tttaaaacca aattggataa acagctttgc
    17461 cggtttgatg caagaacaac cgataccatt taagattcaa tgcagagcag atctattgtt
    17521 ggatgaaaat aatatccaat cacttgccgg agcaggttgc gatgaagtgt ggataggtgc
    17581 cgaaagcggc tcgcaaaaaa ttctcgatgc catggataaa ggcacaacaa tagcacagat
    17641 aaccgaaagc agtcgcctta tgtatcgctc cggaataaag ccttgttttt ttcttcaatt
    17701 cggttatccg ggagaaacat tgactgatat aaagtcaacc atgcaacttt tgcgcaattg
    17761 taaaccctat gatattggta tttccatttc atatccatta ccgggcactg tattttacga
    17821 tcgcgtattt gcgcaattga gtaacaagca aaattggaat gacagcgatg aattattgat
    17881 gatgtatcat ggcacttatt ctgcagcttt ctataaatat ctgcatcgct atgtgcatcg
    17941 aagattccgg atgttacagg gaatgaaatg tatcagagaa attttttcag gcaaacgaaa
    18001 tactacatcg cccccgctaa aaagattatt tgcattacct attcacagtg tactatgttt
    18061 gtattatttc ctcatggtac aaacaaaaaa gcaacctgtg tctgagttac aggttgcttc
    18121 ctaaatattt tattcaatca attatcgaat cagggtgata ttaccggcgc gtttcatttg
    18181 ctgaccgtta tcgaattgca ccgtgatata ataagcatac acgccattat ttgcaggatc
    18241 gccgttaatg gtaccgtccc atcctaaatt aatatcattc gcatcaaggt tttctccgcg
    18301 atacactacc tttccccagc ggtcgaaaat ttcaaaatca gtaatggata aaacgttata
    18361 accaaaaatg tagaagatat cattgatatt atcaccattt ggcgtaaagg tatttgggac
    18421 aaagaaatca tttggtggca atggcaatac ggttatcatc acattatcgc tggcaacgca
    18481 tccattttca tcaaccgatg tcatgatgta atcgattgaa tatggaggca gcacatacga
    18541 ttcagggcaa agcggacact ctactccatt tgttggcgac catgtataat cgccaccgct
    18601 ttcgctatgc agtattgcac ttgatccgta atacaacgtg gtatcgcccc aggcagttac
    18661 gaagttatca tctacagatg tagtaactgt cattgaacta tagcaaccat ttgcatcggt
    18721 atattcatat gtgagggtat atggaccacc aacgccggca tcatttgtca ggaaatgatc
    18781 gccactagtt cccgggccgc tgtaaatacc accgggagga gtagcttcga gtgcaattga
    18841 attctgttcc aaacagaaaa tatcatcaat tggccaggta attacaggta atggattaac
    18901 ggtaaggtta acagtaataa ttgaatcgca accattaatt gatgcgtatg gaaatactgt
    18961 agttccacct gttccggtaa cagaaccatc aggcattgta taagtatcgc cgatacaaat
    19021 ttccggattc atgataatat cataagtcgg attcactgtc aggttagtag taattattga
    19081 atcgcaacct ttagttgtaa gtaagttggt aacataagta cctgtagtat ttacaacgga
    19141 accgtcagga agtgtaaatg ttacatcatc gcaaatcaca gggttttgtg tgattgcata
    19201 aacaggatga acaaacaaag tagtattaat tgttgaatcg caacctgcta cagtagttaa
    19261 cgttacagga taaacgcctg acacaccaac tgctgtgcca tcgggtaaaa tatatgtctc
    19321 gccggaacaa atgtgtacgt tcatattcac aacaggaacc ggtgatactg ttaagttgat
    19381 agtataatta gaatcgcaac cccaaacaga agtaaaattg ttgatataag tacctgttgt
    19441 tgacactact gtaccatcag gcaaggtata tgtagctccg gaacatattg ccggattcaa
    19501 tgtataatta taaacaggat gagcagccag ggttgtagtg attacagaat cgcaaccatg
    19561 aatggtaggt aaagttgtaa cataagttcc ccctgtatac acagttgcgc catcaggtaa
    19621 aataaatgga tcacctaagc agatattacc agattgcgat actgcataag ttggataaat
    19681 tgtaaccgta ttagtagcta cgccatcagg acaaacagta ccgctggcaa cggtattggt
    19741 gatagtatat gttcccggag ttgatgcgct gaggtcaatg gtaccggtag cagcgttaat
    19801 tacaagtccg gcaggtgttg cggtaaaggt acctaatgaa gcacctatca tgggaattgg
    19861 cgacggatca gattcattag cacagaatgg tgatcccggg taactaaatc ctgcagcatc
    19921 cggttcataa atatttactg tgatatccca agttgctgta tcaccgtaac aaattccggt
    19981 aagcaaagta tttgtgagag tataagagcc gggatcagaa gcactaacat cgatagcacc
    20041 tgttgctgca tcaaatacga gacctgaagg tgtacatgta aatgttcctg cggtagcacc
    20101 tgttgcataa agaggagcag ggtcagtacc tgtagtgcaa aattcagctg tgccgccgaa
    20161 tggcactccg ggataggtaa aatcggcaag cacatttgtg actaaacttt tggcctttag
    20221 aaatactcct gaatcccaaa caaaatcaga ttcatcgcca atcactagtt taaggtgata
    20281 ccactcacaa ggagttactt ctgcttctgc agttaacacg gttgtaaaac catcgtattc
    20341 gatatagtat ggcgatgtag cataaattcc cgtaccgccg gtgccattcg gaatatagta
    20401 catcggataa cttgtagcat taacgttatc gatagtaacc ggtaatgttg ttccgggaat
    20461 caaggcaata ttagtaagtg taggataacc cggaccggaa ataaaaaaca caaatgcatc
    20521 attgatggaa cccacatatt cgggatattc ctcagaacca aaaacgtaat cgaatttcaa
    20581 tgaatcgccc ggagcgtata catcaaattc aagcgcacag gcatcatgtg ttgttcctac
    20641 tccggggtaa gcattcagat cagggtcgcc gggccaaccg ttatttgtac cggtgcttcc
    20701 tgaattattt gggcctaaga tattggaaac gcgacctgtt gtcagcacta ctccactatc
    20761 aataccgacg ttacaatcca cgcaatcgaa ttttccgata gccaggttat ggcagttaat
    20821 ggtagcatta tcgatctcaa cgccggcacc tgctaggtat gctgccagtg aatctgctgt
    20881 ccaaccggtg gtataagtaa tctgtgcgtt tactgatagt gcggtaacgc caaagatcat
    20941 caccaagagg agttggcgaa taactattcg ggtcattagt ggtatgtgct tgggttctga
    21001 cacaatatag gcaaaaataa gggttaaatt tacagctctc ttttggaact tccaaacact
    21061 cattagatgc ctcaaacccg cacagtagtt gcattctatg ccgtaatcgc atcaatgctt
    21121 acttttgcct cttgttcaaa ttcaagatcg gcaatgaaaa acgtcattta tacccccaac
    21181 gcccctgctc cgataggtcc ttatagtcag gctaccgagg ccggtggatt tgtatttatc
    21241 agcggccaaa tagccatcaa tcctgcaaac ggcgagatgt ataccggtgg agatattgcg
    21301 gttgaaaccc gtcaagtaat ggaaaatctc aaagccattg ttgaacaatc gggttccacc
    21361 atgggtaatg tgctgaaatg caccattttt ctccgcgata tgaatgattt tgccgcagta
    21421 aatgctgttt atggcgaata ttttcaggaa tacccacctg caagggaaac ggttgcggtt
    21481 gttgggttac cgaagaatgc gagggtggag ataagtgcga tggtgagtag gtgagggggt
    21541 cagtgggtga gtgggtgagt ggatgagtgg atgagtagtg aattcgtgtt gtgtgttttg
    21601 aacatactct gcattgccga aggtagatgg agacacatct gcgaaactcc gctaactccg
    21661 cgattgaaac ccggctaatg ggtgggctga gacatatcta tgaaactccg ctaactccgc
    21721 aaactccgcg attgaacccc ggctaatggg tgggctgaaa catatctatg aaactcctct
    21781 aactccgcaa actccgcgat tgaaccccaa tgagtgaaga gcaaccgcaa cttccaggtc
    21841 tcaaccttca attagtaaga agtatcagca aaatcaaacg cgaataggcc aaatcaaatt
    21901 ccggaaatta ttgcttcacc cactgcgcca cttcaccgcc tgcgcggatg agatacatac
    21961 ctgccgtcaa tgtgctaatg tctgcttctg catatccgtc tgatacagaa atttccatga
    22021 tcagctgacc cagcccgtta tagagttgtg cggtattaat atttgcagag caggtgatat
    22081 ttaactgagt tgttgcaggg ttgggataag cataaaggat ttgagaagga gtttcatttt
    22141 ctccacccct gcatacaata ctcaccatga tcgcattgct gttcttgaag cagcctgtag
    22201 ctgtctttgt taccctacat ttataattac ctgctgttga agcagtatat gcaatattag
    22261 tggcacctgc gatcggtgtt gcccccttat accattgata actcaatcct ccgcctgcat
    22321 tagctgtcaa tacaacactg ccgcctgcgc aaaagctggt tgggccgccg gctgttatac
    22381 ttgcaggggg atttttattt gctgttacac tgatcgatgc agataacgtt gctccgcaag
    22441 cattacttga ttcacaagta taaatacctg tttttgttgc agcatatgta actccggttg
    22501 caccgggaat tgttgttcca tttttcttcc attgcaatcc ggtaccggtg tgcgttgcag
    22561 taagaatgac attactacct tggcagaaag ttgttgcacc tccggctgaa atagaaattg
    22621 ctggagatgc accttcgtca atgagaccgt cgcagtcgtc atcaattagg ttacacattt
    22681 ccggagcagg tgtacatgca gggccggcta gttttacaac ccaataatcc catgtaccac
    22741 caaaactatt ttccgattta tcgcccgaga tattagactg agaatacccc cctagaaaaa
    22801 acccgccatc catagtttgt attgaacacg acaatttatc ccagtagcta gctccaatgt
    22861 tatattgcca ctgaatcgca ccaatatcat taatttttag cacccaatag tcgtagtcgt
    22921 gagctacata tacattttct gtcttgtctc cagaaatatt tgattgcgag taaccagcaa
    22981 caaaatattg accatcactc aattgtataa cagaggctat gttttccgct tcggatccgc
    23041 caattgtatt ttgccattca ataattccaa atgaattgag cttaataatc caataatctg
    23101 aatacccaat actatcttca gttttatctc cggatatact tgaggtggat tgcgccccaa
    23161 taatacatcc accgtctgaa gtaacattca cacttcgaag cttgtcatct aggtttcctc
    23221 caattgtatt ttcccattca actactccca gcgaatttaa ttttagaagc cataggtcgt
    23281 tgtcgccaat attattttcg cttttatcac cagaagcgcc tgaaaaggaa tatcccccaa
    23341 cataatacgt tccatccggc aattgcgcaa cagactgtaa aacgtcagta ccggaacccc
    23401 ctattgtatt ttgccactcg atattgccta gattattaag ctttataacc caataatcct
    23461 gcgatccatt ggagttttca gttttatctc cgcttatgtt agaaaacgat ttacctccta
    23521 caaaatatcc accatccgct gtttgtatca cacattctaa ataatcatag ttgtttcctc
    23581 caattgtatt ttgcc
//
LOCUS       DAOQHY010000032        23585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_949_length_23585_cov_6.276413, whole genome shotgun sequence.
ACCESSION   DAOQHY010000032 DAOQHY010000000
VERSION     DAOQHY010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 23585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23585
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_949_length_23585_cov_6.276413"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..610)
                     /locus_tag="PLL28_04310"
     CDS             complement(74..610)
                     /locus_tag="PLL28_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68574.1"
                     /translation="MRNTQAQVLQADTLPQSEYLNFYISASSEDFYIKFTQVPNKLFG
                     ESLKVRINNESGVFAIPIINEENYSIDLPGRSSPYRSDTLYFYVEMYDQVYHSIAVVI
                     FPGFMRNISFGEGCYELDCTPVQDPYMPSVVVPFLIDPDAAYKIPGRNKIITGKKALS
                     RYTPCDLEKFEQVFFQDN"
     gene            complement(812..1276)
                     /locus_tag="PLL28_04315"
     CDS             complement(812..1276)
                     /locus_tag="PLL28_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68575.1"
                     /translation="MKSLLIIGVLTLSCMSKLHAQKINPDYDSTLAKQLNADDYGMKS
                     YILVILKTGSNNVKDKQLRDSLFAGHFANMSAMVEAGKLVVAGPIDKNENNYRGIFIL
                     NVTSFEDAQLLLQNDPTIHEKVLEAELYKWYGSAALPVYLDTHDKIQKFSIE"
     gene            complement(1400..2041)
                     /locus_tag="PLL28_04320"
     CDS             complement(1400..2041)
                     /locus_tag="PLL28_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68576.1"
                     /translation="MSYINLRYGKQEDVDAMMYIKHQLPLKTADGNPVKGGFLLGTTR
                     EQYLSFILNDHCLVAEDHGNVVGFGIMLHNASLRNSDIWQRRHQAAWEVDINDFEDLP
                     ICYFEQLAFLPGYNRAVLKLAYQLVMDAFRQGHTHLFATTVHKPVLNIAAVPFIKKAD
                     GGLVGRIDEYYPEVGNILSDIYLIDRIQFYDAVPKLAIYPWLIKSLAGDSKLV"
     gene            complement(2022..2951)
                     /locus_tag="PLL28_04325"
     CDS             complement(2022..2951)
                     /locus_tag="PLL28_04325"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNF68577.1"
                     /translation="MIPRRNGILLVETFTILILNGVLATYMHSHLPTTLFRCCYAPLL
                     LFQGLWYYRIYIVGHEAAHKKLIPAHLLLNEIAGSLVLLPLLTPLNIYRKIHYFHHGF
                     NRKDPHTSALDTFVVKGKYRTLKTAYYYVVWYLSVFCGGFFLHSLVSVILFLFCPPAL
                     ARKISPAFNGWNYKDQLKAILLFLAGILIHLWVFTFFGKTAYLLSLGLPMLVFAWLLS
                     MLVYVFHYDTTMGEHVRYNVRSVNRIPVLSWILMNFNEHATHHQYPNIPWYSLPENRS
                     ELPEEFGNKNQSTRNLFVAVIRQLKGPHVIYQP"
     gene            complement(3142..4020)
                     /locus_tag="PLL28_04330"
     CDS             complement(3142..4020)
                     /locus_tag="PLL28_04330"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68578.1"
                     /translation="MKTLAFFLAFSYLSSRLFSQVYIIPNAELQPSWTFPLCFEDASG
                     ARDTIYMAYDIDAIDYSIPSADTIFGEAFLPIDTSEFRAYWEFIYDGKAVKTLVMKNF
                     IPAQIKFNKAQLPVKFTWDAQLFYSENLPFPNNYPLPNAWGLVHCSSWKDFEGCQLDE
                     YSGIYLTNEPDFVYTDVATDSILYQSEYYYFFENGLDFRILQYGTGYNWATIVENESQ
                     SFQIYPNPSDDFIHILTKENTKATLIISDMQGKTIKQFADVSNATIPVYDLLPGMYMV
                     NYSSYESAYSSKFIKK"
     gene            4242..5711
                     /gene="guaB"
                     /locus_tag="PLL28_04335"
     CDS             4242..5711
                     /gene="guaB"
                     /locus_tag="PLL28_04335"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNF68579.1"
                     /translation="MEHLSTKFLGESLTFDDLLVVPAYSEVLPREVDISTRLTRNLRI
                     NVPIVSAAMDTVTESQMAIAMAREGGIGIIHKNMTIEKQADQVRRVKRSDSGLIIDPV
                     TLSKTATLRDALQMMREFKIGGIPVVDDNQKLVGILTNRDLRFETELSRPVSEVMTKE
                     NLITAPEGTTLAQAEDILRNYRIEKLPVVDASGTLKGLITYKDIKKLLNHPRACKDSF
                     GRLLVGAGVGVTHDTLQRVEALRSVGVDLVIIDTAHGHSRGVLDMVRLVKKTFPDLEI
                     VAGNVATGSGAKALADAGVDGVKVGVGPGSICTTRVVAGVGVAQVSAIYDAAQALKGT
                     GVPVIGDGGIRYTGDIVKAIVAGADTIMAGSLLAGTEESPGETIIYEGRKFKSYRGMG
                     SLEAMEKGSKDRYFQDVEDDLKKLVPEGIVGRVPFKGTVEEVMVQYIGGLRAGMGYAG
                     ARTIEDLKTAQFVKITASGIRESHPHDVVITKEAPNYSR"
     gene            5872..6255
                     /locus_tag="PLL28_04340"
     CDS             5872..6255
                     /locus_tag="PLL28_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNF68580.1"
                     /translation="MKKLIALTISLLMLVSMQAQQRNDIQVGGNQTQMKDGCGAPVGV
                     FVDEITLISATIHWNTNGAAYYKVQFYNVNNPREKGTLMTNLGSVMLDNLTPCATYRF
                     IITAKCADGLSKTQAMSFSTKGCSN"
     gene            complement(6398..7726)
                     /locus_tag="PLL28_04345"
     CDS             complement(6398..7726)
                     /locus_tag="PLL28_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNF68581.1"
                     /translation="MTRNFVAAVMLLMSTVALNAQVQAPEWSKDKVIYEVNIRQYTPE
                     GTFNAFTAQLPRLKQMGVGVLWLMPVQPIGELNRKGSLGSYYSISNYVTTNPEFGTLK
                     DFKKLVDEAHKLGMYVILDWVGNHTAWDHPWITDHPEYYTTDKNGKIQPPVADWTDVA
                     DLNYDNMQLRKAMIHDMAFWVKETNIDGFRCDVAMMIPDAFWQYAFNEMREIKPELFM
                     LCEAEGPQFVKDGFNMVYGWERHHAMNQIAKGKGTVDHLDSLVQKDIASYAPISYYMN
                     FTSNHDENSWNGTEFERMGDGARAFAVLSATLPGMLLIYSGQEVGLDRRLKFFDKDSI
                     NWVDDKQFTPFYTKLINIKTQNQALWNGNYGGSYRRVACENAGTYIFAREKDGNKVLV
                     VANLTAEISNIVFANEAGKYIDAMSGKKEKVKATEKMTLKPWEVKVLVKK"
     gene            complement(7754..10171)
                     /locus_tag="PLL28_04350"
     CDS             complement(7754..10171)
                     /locus_tag="PLL28_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNF68582.1"
                     /translation="MLSRLRFSFLQIGILFVLTSILLPAASQTLSFGLMQPITLSGDT
                     TMVITRDYILQPSMISNVTTPDFLQARLSDNKDTLLLIRNGREMQTLSTLHFSAGNEM
                     FDVLLKKPRKIPVSISFDPNGKKYTQVNIVGDLTNWNPNVLHMQMVNGKWVLPLQLNP
                     GRYQYQIVADGNWFLDPGNKDSIDNGIGGYNSLLNVGGSEGDAPDIYTVWEDSLTNTL
                     DVSGSAEHYVVFWDNHQLTYKEIYSQHEEDFSEGKDIVIAVPEQAKSVKRSQIRIYAY
                     RGNLISNDLLIPLEYGKVITDSKLLDRTDFQKTIMYFMMVDRFVNGDKSNDHPVDDAR
                     VMPQANYQGGDLAGVKQKIDDGYFEKLGVNTLWISPVFQNPMGAYQEFPEPHRWFSGY
                     HGYWPISLSKVDVRFGTNAEFKSLVDDAHGNDMNILLDYVANHIHAEHPLWTSHPEWF
                     SQLDLPDGRKNLRMWDENRLTTWFEPYMPSFDHSQPAVAEACADSAMYWLTTFGIDGF
                     RHDATKHIETEFWRLLTFKIKTEVAIPQQKPIYQIGETFGSRELIGSYIGSGLLDAQF
                     DFDLYFDARAVFASGDIPFSRLSSSLKESFDYFGWHHVMGNISGNHDMARFISYASGD
                     LKWNENAKEAGWNRHITITDTIGYARLKMLQAFNMTIPGIPIIYYGDEIGIPGGDDPD
                     NRRMMYFDSLNRFEKDVFNTVSKLTQLRSNHMALLFGDFEVLQEQSAGSNDLQFAYLR
                     SYFNDAVIVVFNRSNEQVTLSYPLPTRLLNAIPQAQMGTTINRHGYQIDILDIPANSF
                     EIITFDR"
     gene            complement(10281..12302)
                     /locus_tag="PLL28_04355"
     CDS             complement(10281..12302)
                     /locus_tag="PLL28_04355"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNF68583.1"
                     /translation="MSQSADLTGQFREQILNGIPSAIPAAKPYQQGINHAPKRKDILT
                     KEEKSLALRNALRYFPKHMHADLAPEFAEELKTYGRIYMYRFRPEYVIKARHYKDYPH
                     NSVQAACIMLMLQNNLDDAVAQHPHELITYGGNGAVFQNWAQYLLAMKYLATMTDEQT
                     LVLYSGHPMGLFPSHKNAPRVVVTNGMMIPNYSKPDDWEKFNALGVTQYGQMTAGSFM
                     YIGPQGIVHGTNITVLNAARKIDTHAQDCAGKVFVTAGLGGMSGAQPKATVIAKGVCV
                     VAEVNPEATTKRHAQGWVDEVFTDLDTLISRMQEARKNKEAVSLAYQGNVVDLWEKLA
                     NDNIQIEIGSDQTSLHNPFAGGYYPAGLSFEESKRMMAEEPELYKQKVYESLRRHVAA
                     INKLTAQGMYFFDYGNAFLLEASRAGADIMLPNGDFKYKSYVQDILGPMCFDYGFGPF
                     RWVCCSNDEKDLRLTDMIAAEVMETIYADAPAEIKLQMSDNINWIKSAMENKMVVGSQ
                     ARILYADAEGRIKIAQAFNKAVREGKLSAPVVLGRDHHDVSGTDSPYRETSNIYDGSR
                     FTADMAIHNVIGDSFRGATWVSIHNGGGVGWGEVINGGFGMVLDGSEAADRNLQMMLF
                     WDVNNGIARRSWARNKEAMFAIDREMHRTPELTVTMPQMVDMDLDKFWE"
     gene            12400..12828
                     /locus_tag="PLL28_04360"
     CDS             12400..12828
                     /locus_tag="PLL28_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68584.1"
                     /translation="MKKLLLATTVFAFGLVACDRNEIPDYDVRDEYVGMYDVTDACDA
                     MLNDYTLEVYKGSSYDEIVFGWPGLYEAGMDVTGIVTGMKVVIPIQQFTISTYPNIFY
                     EFSGSASLEDSILTVDYTVLTVQDGLIIDEHNCIATMERN"
     gene            complement(12825..14048)
                     /locus_tag="PLL28_04365"
     CDS             complement(12825..14048)
                     /locus_tag="PLL28_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNF68585.1"
                     /translation="MRVFVTMVLLIAGTIGFAQSGFTIYNQDNSDLPTNLVNAITIDD
                     HNNTWVGTEGGLVKIDPVGTWTTFTTANSGIPDNQIRSIFIDENDVVWVGTYLDGLAS
                     YDGTDWVAYDPTNSGLPDYHIKSIAKDANDTMWIGTSWGLAKWDGADYWYTYTELNSD
                     ILVPNINALYIDSNDVVYIGTLNGGLNVYDHGVFTIYRTANSTISDNTILSIDEDIYH
                     NKWLATSFGGLSVFTEAGDFLKFTPLTSDISDWSVDAVRLDNNNLGVIGMSATGLEIF
                     NNISWTHYTTANSDLPDDFINALAIDSANIIWIGTEEGGLVKFDRSAVAVNTIGQVEV
                     LTYPNPATDNALIIGLPENLSVQIYNNQGMLCGIAEYRNGMHIVNAAKLSAGTYWIYA
                     VDQHTTYTASLQVVH"
     gene            complement(14181..17606)
                     /locus_tag="PLL28_04370"
     CDS             complement(14181..17606)
                     /locus_tag="PLL28_04370"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNF68586.1"
                     /translation="MKDIFKEFKPSSWAIDNRTTIFVITVILTFMGISTYNALPKELF
                     PDITMPTVYVSTIYPGSSPSDMENLITKELEKDIAAISGVNKITSNSVQDFSSIQVEF
                     ISGTDINDANQKVKDAVDKAKPRLPSDLDPTFGPNVMKVEFSEIPIMQVNVSGDYDLG
                     SLKKYAESLQDKIEELPQISRVDIIGAPEREIQINVDMYKMQAAQLTMYDVYAAISNE
                     NRIISGGTMKVENMRRSITVSGEFDKIEQIQNLTINNANNYPIYLKDVAQIKDDFKEQ
                     ESFARLDNKKVITLNVIKKSGENLIEASDNINTLLEDVKAKDIPGDVNITITGDTSTT
                     TRTQVADLINTIIIGFLLVTIVLMFFMGATDAMFVGLSVPLSMFIAFVVLSLLGMSMN
                     VIVLFGFLLGLGIVVDDAIVVIENTHRIFANGKVPIKTAAKNAAGEVFLPVLAGTMTT
                     LAPFFPLLFWPGMIGSFMKGLPITLIITLTASLIVAYLINPVFAAAFMKPHHDHEKPN
                     RRRHLIWSAILIAVGLLFDASGKMLFGNIVISLVAVFWFYNLVLIHAVRWFQKKGWPA
                     FQHRYASILRWTLNHPGKIFLGILGTLILAIALIVVTKPGVEQFPSGDPNFIYTYVVM
                     PTGTDQQTTDSVTQIVEKRIYEVIGKDNPLVKSVISNVTIGASEDPFDQSAASNKGKV
                     GIAFIDPSERRHQKAEPYIEKIREAVKDIPGADITVGVEQNGPPAGKPINIELTGENF
                     DDLIKTSELVKRYLDSLNFAGIEELKTDLQANKPEIVIDLDRERMNREGISTLTVGGE
                     IRNAVYGWEVSKFKDGNDDFPIIVRYDESQRKNIDQLKDLKITYRDMTTGQIRQIPLS
                     AFATIDYASTYSAIKRIDQKRVVTVSSNLLTGYESQQPVIMNQIKAALEGFDTPEGVT
                     IGYTGASQDMEETFSFLGVAWLISLFLIVIILVAQFNSISKPIIIFAEVLFSIIGVLL
                     GYGLFGMDFSAVMTGVGIVALLGIVVRNGILLVEFTDILIAEGVPLREAVVEAARIRM
                     TPVVLTATATILGMIPLAIGFNIDFVGLFTHGKPDIWLGGENVVFWGPLAWTIVFGLG
                     FATFITLLVVPVMYLLNERFKAWVFPLFGFTYKHKVSDTHGKAKASMELLDLD"
     gene            complement(17696..18811)
                     /locus_tag="PLL28_04375"
     CDS             complement(17696..18811)
                     /locus_tag="PLL28_04375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNF68587.1"
                     /translation="MKKNFIYLAMALFIAACSPKESGDKKAELEALKKERTALDKQIK
                     ELEAEIAKTDTTKSKDPELVVVTEIQPQEFTHYIEIQGKIDADENVLVTPEMPGVIKA
                     VYVVAGQSVSKGQLLAELDNDAMRKNIETLESQLGFAKEMYNKQKALWDQKIGSEVQY
                     LSAKNNVETLEKSIAAAREQLNMSKLTSPISGLVDNVDLKAGQIGSPGFQGIRVVNMS
                     SLKAKGEVSESHSNVVSTGDNAKIIITDLNKEIDTKVSFAARVINPQSRTFSVEARIT
                     GESDLKPNMIAVIKIADYEAKEAMVVDLNMIQHSSDASFIYVAADKEGKKIAQRRQIT
                     VGKTYDGHAEIVSGIQKGDRVVTSGYQNLIDGQEIKF"
     gene            complement(18824..20116)
                     /locus_tag="PLL28_04380"
     CDS             complement(18824..20116)
                     /locus_tag="PLL28_04380"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNF68588.1"
                     /translation="MAVLLLPAILQAQTMRFSLQEAIDYGLKNNPQIKSAELDVQNAE
                     ITVDNRIASGLPQVDAKLDYQNFFVLPTSLIPGEFFGGEPGTFIPVQFGTEQNMTAGI
                     SASQLLFNGAWLVGVSAAKQYAALVQTQSKIALSDVHKNVESAYNSVLVAQEFEKLLQ
                     KNISNLDKVLFEVTELNKQGFVEGIDVDRLTISKTTLQGQLDNAKRQTALAAQYLKFS
                     MGLDMSTAIELTDTLAPIKEVNMAGSNYLARPEFAVMESLVQLNALNIKVNKAMYLPS
                     LVLYGSYSQNAQRNEFDFLSGDGQWFETGLVGLTLSVPVFSGMQRRSNVQMAQVAYEK
                     AKLDQFTATQGILLEVEKAKTDYMNAANDLEVQKKNIALAQKIYDTALIKYREGVGSS
                     LELSTAESTLYQAQGSYIGALFNVLNTQTSLNKALGNY"
     gene            complement(20133..20750)
                     /locus_tag="PLL28_04385"
     CDS             complement(20133..20750)
                     /locus_tag="PLL28_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNF68589.1"
                     /translation="MNEEVKKILNRCERLFMKYGIKSVTMDDVARELGVSKKTLYQYF
                     ENKEQLIQTVTENHFKCQNEMVQQVIHHSKTAIDEMYSIAAWMNAMSKNLNPALLYDL
                     RKYHPESWQVFIDHRNNEVLNSIRHNLERGINEGLYRADLDIEVIARIYITRVEMFID
                     NEIFPLDKFSPERTFNVFIDYHLRGIATPKGIKYLEQLKSQNHGI"
     gene            complement(20941..22104)
                     /locus_tag="PLL28_04390"
     CDS             complement(20941..22104)
                     /locus_tag="PLL28_04390"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNF68590.1"
                     /translation="MRFLSADVIYPSYGNPLREHVLIVHHDGRIDGIVAKADIDPLKI
                     EHFDGALCPGFVNAHCHLELSHLLGKFPMKTGLPAFLMEVTRQRETAMEDIISAMQEA
                     DSSMWQNGIVAVGDISNAPHSLDIKADSRIYYHTFVELLALDPQKATTAIEHGNAIVA
                     LAKERNLSASLAPHAPYTISTLLVEGITRACRDMGMPTSIHMLESNDENEFYVQATGG
                     YRKLYRELGIPITHFEPTGKTSLESILPFMDKDVKTLLVHNTIATVWDADWAENLHAN
                     LYWCFCPNANLFIEDRLPDIPELIQHIKHIAIGTDSLASNHGLSILDELKTIAHTFPS
                     LTTEELLQWATDGGAQFLGLSDRFGILKPGLSPGILHLTTSEIQPETLVNRLF"
     gene            complement(22207..22857)
                     /locus_tag="PLL28_04395"
     CDS             complement(22207..22857)
                     /locus_tag="PLL28_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68591.1"
                     /translation="MKKLIIACMVLCTITVKAQSLESGTLSFQVGYDAMIHGTASETT
                     FFGVVVDRDTSGAVTSMFNADAQYSFAPWFSAGVAFNIGSYLEDTADQSNDGNHVSII
                     AFDARLYPVNNDKFNWYLGGSAGLTNLEINKKDALTPGVNYQYKYKSPALGLFTGFNW
                     YFFDVAGVFLQFEYSQQNFDLKSFSIDGAEQDLSQTTQSLITKGGGIRLGVNFKLN"
     gene            complement(22971..>23585)
                     /locus_tag="PLL28_04400"
     CDS             complement(22971..>23585)
                     /locus_tag="PLL28_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68592.1"
                     /translation="DGTLYTSLYDTKRRLISLCFYHNYDRVHILSLNDELAKGEHWIA
                     METLFPKNEEFETLKSYITPANHITLLPIMLIVVILLLGMAAILSTQWFRQLHLAEKN
                     LQYLRISIAIFNALLAIYIWVIMNEPAVYYFPAPYFEAGRGWINLLSYMPVLTLIVMA
                     ATGWLVFRKQVVTTTGARVGFYVNAVVWLLCAIAFAYWGLIAII"
BASE COUNT         6989 a         5012 c         4597 g         6987 t
ORIGIN      
        1 gtggagaggc aggaatccgc tgagagatga tgaagtttga gttatcaccg gatgcggcga
       61 tgctcattga gcactaatta tcctgaaaaa acacttgctc aaatttttct aaatcgcagg
      121 gagtatacct gctgagtgcc tttttgccgg taatgatctt gttccttccg ggtatcttgt
      181 aggcagcatc ggggtcgatg aggaatggta cgacgacaga aggcatatag ggatcctgta
      241 ccggtgtgca atcgagttca tagcagccct caccaaagga gatattgcgc ataaaaccgg
      301 ggaatattac cacagcaata gaatgatata cctggtcgta catttcaaca tagaagtaga
      361 gtgtatcaga cctataggga cttgatctgc cgggtaaatc aatactgtaa ttttcttcat
      421 taatgatcgg aattgcaaaa acgcctgatt cattgtttat tcgcactttc aaactctcac
      481 caaagagctt gttaggcact tgtgtaaatt tgatgtagaa atcttcgctg ctcgcactga
      541 tataaaaatt caagtattca gattgcggga gtgtatcagc ctgcaggacc tgcgcttgcg
      601 tgtttctcac acaaatgaaa aaaatgaacg ataatattgc ttgaaaacat aaccgcaaga
      661 agctgagatc cattttaaga tattatatag attgaatttt aaagtaatac ccggaggatt
      721 tttggattta attcaacaac tcatgcaatc attcccaacg cagccccaat aattactcct
      781 attccccttt ctcctttctc ctctctcctt tctactctat tgaaaatttc tgtattttat
      841 catgtgtatc cagataaacc ggcaaagctg ctgatccata ccatttatac agttcagctt
      901 caagcacttt ttcatgaata gtagggtcat tttgaagtag taattgggca tcttcaaagg
      961 aggtaacgtt taggatgaaa attccgcgat aattattttc attcttatcg attggtcccg
     1021 ctacaaccag tttaccggcc tcgaccatag cagacatatt tgcaaaatgc ccggcgaaga
     1081 ggctatcgcg aagttgttta tcctttacat tattggaccc ggtttttaaa atcaccagta
     1141 tataggattt catgccatag tcatcggcat tcaattgttt agctaaggtc gaatcatagt
     1201 caggattaat tttctgagca tgcaatttgc tcatacaaga caatgtcagc actccaatta
     1261 taagtaatga tttcatatta aaattatcac accaaatata aagcacaggt atcataaaat
     1321 gtgaacgaca acataaatta acagtaagat tgcaattata tctatacaaa atgatcagta
     1381 atacaaagaa tgatagtgtt tacaccaatt tactatcacc cgcaagactc ttgatcaacc
     1441 atggataaat tgcgagtttg ggcactgcat cataaaactg aatcctatct atcagataaa
     1501 tgtcggaaag tatatttccc acttccggat aatattcatc tattctcccc accaggccgc
     1561 catcagcctt ttttataaaa ggcactgctg caatatttaa cacaggcttg tgtacggtgg
     1621 tagcaaataa gtgtgtgtgt ccctggcgaa atgcgtccat aaccaattga tatgctaatt
     1681 tcaaaacggc tctattataa cccggcagaa aagcgagttg ttcaaaatag catattggca
     1741 gatcttcaaa atcattaata tctacttccc atgcagcctg atgtcgcctt tgccaaatat
     1801 cggaattacg cagtgatgca ttatgtaaca tgataccaaa accaactaca tttccatgat
     1861 cttcagctac gagacaatga tcatttaaaa taaatgagag gtattgttcc cgggtcgtgc
     1921 caagtaaaaa cccaccttta acaggatttc catcggctgt ttttaaaggg agttgatgct
     1981 taatgtacat catagcatcc acatcctcct gcttgccgta tctaaggttg atatatgaca
     2041 tgaggtccct ttaattgacg aataacggct acaaataaat ttcgggtaga ttggttttta
     2101 tttccaaact cctcaggcaa ttcggagcga ttttcaggta aactgtacca tggtatattc
     2161 ggatattgat gatgtgtagc gtgttcattg aaattcatga gtatccagga taaaacaggt
     2221 atacgattca ccgagcgcac attatatcga acatgctctc ccattgttgt gtcgtagtga
     2281 aaaacataaa cgagcattga caacagccag gcaaagacaa gcataggtaa tccaagactc
     2341 agcaaataag cagttttacc aaagaatgta aatacccata aatgaattaa aattccggcc
     2401 agaaataaca atatagcttt taattgatct ttataattcc aaccattgaa tgccggagat
     2461 atttttcggg cgagtgcggg gggacagaac aaaaataaaa ttacagatac taatgaatgt
     2521 aaaaaaaagc cgccgcaaaa cacacttaaa taccaaacga cataataata tgcagttttc
     2581 aatgtgcgat attttccttt taccacgaat gtatctaagg cagaagtatg aggatctttg
     2641 cgattgaatc catggtggaa atagtgaatt ttgcgataaa tatttaaggg tgtcagtaat
     2701 ggcaggagca ctaatgatcc ggctatttca ttcaataaaa gatgtgcagg gattaatttt
     2761 ttatgagctg cttcatgtcc aacaatataa atgcgataat accacagtcc ctgaaataat
     2821 agtaagggtg cataacaaca tcggaacagg gtagttggaa gatgcgaatg catgtaggtt
     2881 gccaacaccc cattcaaaat caggattgta aatgtttcga cgagcaggat gccgttccgg
     2941 cgtggaatca tgttggataa atgatgaata atttcaattc aaattaacgg aaagttttgc
     3001 aaatcaaatg tagtccgggg tctaataaag tgtaaaactt tcttaataga aaatcttata
     3061 caataaatgc atgtagcaaa aacggttgta ttgcaaaagg attgatactt ttacaactaa
     3121 ttgaaaatta aagattcttg attacttctt aataaatttt gacgaataag cagattcata
     3181 actactatag tttaccatat acattccggg caacaaatca tagacaggaa tagtggcatt
     3241 agatacatcg gcaaactgct tgattgtttt tccctgcata tcactaataa ttaatgtggc
     3301 cttggtattt tcctttgtta aaatatgtat aaaatcgtca ctcggatttg gataaatttg
     3361 gaatgactgg gattcatttt caacaatagt tgcccaatta taacctgtac catattggag
     3421 tattcgaaag tctaacccat tctcaaaaaa ataataatat tctgattgat acaatatact
     3481 gtctgtagca acatctgtat acacaaaatc cggttcattg gtcagatata tgccggagta
     3541 ttcatccagc tgacaacctt caaaatcttt ccaagaactg caatggacca atccccatgc
     3601 atttggcaag gggtagttgt ttggaaatgg taggttttcg gaataaaaca attgagcatc
     3661 ccatgtaaat ttcacgggca actgtgcctt attaaattta atttgtgcag gaataaaatt
     3721 cttcattaca agcgtcttca ctgcttttcc atcgtaaata aactcccaat aggctctaaa
     3781 ttccgaagta tctattggta aaaacgcttc gccgaatatg gtgtcggctg aaggaatgct
     3841 atagtcaatt gcatcaatat cgtaggccat atatattgta tctctggcgc cggaggcgtc
     3901 ttcaaaacac aatgggaaag tccaagatgg ttgtaattcg gcattaggaa taatatagac
     3961 ctgcgagaaa agtcttgagg ataaataaga aaaggcgagg aaaaacgcta aggttttcat
     4021 gtattaaaaa tatagaaatt ttgggagaga gagtgggaga tatctaagcg gtttgattcg
     4081 gatataagcg cttcatcgct ctaaaccttt cgtgtttaag acctaacaga tcttaaacct
     4141 aacaggtctt agacctgtta ggtttaagat ctgggggctc attttaaggg tcaaaacacc
     4201 cccaatttgc atctcccgaa aaatatgccg tatctttgca catggaacat ctctcaacca
     4261 agtttctggg cgaaagcctt actttcgacg atcttcttgt tgtacctgcc tactctgagg
     4321 tccttcccag agaagtagat atttctacac gccttactcg aaatctgcgt atcaatgtgc
     4381 ccatcgtatc ggctgccatg gataccgtaa ccgaatcgca aatggctatt gcaatggctc
     4441 gtgagggagg catcggcatc attcacaaaa acatgaccat cgaaaaacaa gccgatcagg
     4501 tgcgtcgtgt aaaacgcagc gacagtggac tcataatcga tcccgtaacc ttgtcgaaaa
     4561 cggctacact aagagatgca ctccaaatga tgcgcgaatt caaaattggc ggcattcctg
     4621 tggttgacga taatcaaaaa ctggtgggga ttctcactaa tcgcgacctg cgatttgaaa
     4681 cggaactgag ccgaccggta tcggaggtga tgacaaaaga aaatcttatc accgctcccg
     4741 aaggcactac gctagctcaa gccgaagaca ttttacgcaa ttatcgcatc gaaaaactcc
     4801 cggttgtaga tgcttccggc acgctaaaag gtcttatcac ttataaggat attaaaaagc
     4861 tactgaacca cccaagagca tgcaaagaca gctttggccg actgctagtt ggtgccggag
     4921 ttggcgttac acacgatact ctccaacgcg tagaggcttt gcgcagcgtg ggtgtcgacc
     4981 tggtgatcat agatacggca catggccatt cacgtggcgt tttggatatg gtgagattgg
     5041 tgaaaaagac cttcccggac ctcgagatcg ttgccggaaa cgtggctacc ggctccggag
     5101 caaaagcact tgccgatgct ggtgttgatg gcgtaaaagt aggtgttggc cccggaagca
     5161 tctgtaccac aagggttgtg gcaggtgttg gtgtagctca ggttagtgca atttacgacg
     5221 ctgctcaggc gctgaaagga acaggcgttc cggttattgg cgatggcggt atccgctaca
     5281 ccggtgatat tgtaaaagct attgttgccg gcgctgatac cattatggca ggaagtctgc
     5341 ttgcaggaac agaagaaagt ccgggtgaaa ctatcatcta cgaaggccga aaattcaaat
     5401 cttatcgcgg aatgggtagt ttggaagcca tggaaaaagg gtcgaaagac aggtattttc
     5461 aagatgtaga agatgacctc aaaaaacttg ttcctgaagg aattgttggc cgcgtgccat
     5521 ttaaaggaac tgttgaagaa gtcatggtac aatatatcgg cggattacgc gcaggaatgg
     5581 gatatgcagg tgcacgaaca attgaagacc tgaaaacagc tcaatttgtg aagattaccg
     5641 cttccggtat tcgcgaatct catcctcatg atgtggtaat aacaaaagaa gcacctaatt
     5701 atagtagata ggagagagga gagaggaaag aggagagagg agaaaggaga gaggagaggg
     5761 ttgctttaga tctgaaaaat tttttgatga ggataatcac cgtttgatgg tatcggtggt
     5821 cattgatgtg cctatgtaat agcatgaact ttggtccata aacacaaagt catgaaaaag
     5881 ctcatagcac ttacaatttc gctgttgatg ctggtaagca tgcaagcaca acaacgcaat
     5941 gatatacaag ttggtggaaa ccaaacacaa atgaaagacg gttgcggcgc accggtaggt
     6001 gtatttgttg acgagattac gctcatttct gcgaccatac attggaatac aaacggagct
     6061 gcgtattata aagtgcagtt ttacaatgta aacaatccgc gagaaaaagg cacactgatg
     6121 accaatctcg gaagcgtcat gctcgacaat ctgacccctt gtgccaccta tcgattcatc
     6181 attactgcaa aatgcgctga tggattgagc aaaacacaag caatgtcatt cagcactaag
     6241 ggatgtagca attaggtaac cacctcgtaa tacttgaaca aaaaaagcgc ctctagtgaa
     6301 gcgctttttt tgtatgtatc attttattaa ttacaaaaaa atataaatta atttcgagaa
     6361 atgaatattg attgttattc aactaaatta ctaaaagtta ttttttgacc aacaccttca
     6421 cctcccaagg tttaagcgtc attttctctg tcgcttttac tttttccttt ttaccggaca
     6481 ttgcatcaat gtactttcct gcttcgttcg cgaaaacaat attagaaatt tcggcggtca
     6541 aattagcaac aaccaatact ttattgccat ctttttcacg ggcaaaaata taggtgcctg
     6601 cattttcaca tgcaacgcga cgataagatc cgccataatt accattccat agtgcctgat
     6661 tttgagtttt gatgttaatc agttttgtat aaaatggcgt gaattgctta tcatcaaccc
     6721 aattaatact gtccttgtca aaaaatttca atctacgatc aagtccaact tcctgaccac
     6781 tatatatcag caacattcca gggagtgtgg ccgacaatac ggcgaatgct ctcgcaccat
     6841 cacccatgcg ttcgaattct gttccattcc atgaattttc atcatggttg ctggtgaaat
     6901 tcatataata acttatgggt gcatagctgg caatatcttt ttgtacaaga ctatctaaat
     6961 gatcaaccgt gccttttcct tttgctatct gattcatggc atgatgacgt tcccagccat
     7021 aaaccatatt aaatccatct ttcacaaact gtggtccttc cgcttcacaa agcatgaata
     7081 gttccggttt aatctcacgc atttcattga aggcatattg ccagaatgca tccggtatca
     7141 tcatcgcaac atcgcatcta aatccatcaa tattcgtttc cttcacccaa aatgccatgt
     7201 catgtatcat tgccttacgc aattgcatat tatcgtaatt caaatcagcc acatctgtcc
     7261 agtcggcaac cggtggctga attttgccat tcttatcagt tgtataatat tcaggatgat
     7321 cagtaatcca tggatggtcc catgcagtgt gatttcccac ccaatcaagt attacataca
     7381 tgcctaattt atgcgcctcg tcaactagtt tcttaaaatc cttcaaggtt ccaaactccg
     7441 gattggttgt cacataatta gagatagagt aataactgcc taaagatcct ttcctgttaa
     7501 gttcaccaat aggttgaaca ggcattagcc aaagtacgcc aacacccatt tgtttcaaac
     7561 gcggcaactg agcagtaaaa gcattaaaag ttccttccgg tgtgtattga cgaatattaa
     7621 cttcgtaaat cactttatcc ttactccatt ccggagcctg cacctgtgca ttgagcgcaa
     7681 cagtcgacat aagcagcatc acagctgcca caaaatttct ggtcatgtaa ttaataaaaa
     7741 tttagaacgt gaattatcta tcaaaagtaa ttatttcaaa cgaatttgca ggaatatcca
     7801 atatatcgat ctggtagcca tgcctgttta tagttgtgcc catttgcgct tggggaattg
     7861 cgttcaacaa cctggtaggc aacggataag acagcgtcac ctgttcgttg cttctgttga
     7921 atacaacaat cacagcatca ttgaaataac tacggaggta agcaaattgc aaatcattgc
     7981 taccggctga ctgttcctgc agaacttcga aatcgccaaa cagcaatgcc atatgattgc
     8041 tacgcagttg agtgagcttc gaaacggtat tgaatacatc tttctcaaat cgatttagac
     8101 tatcgaagta catcatacgt ctgttgtcag gatcatctcc accgggtatc cctatttcat
     8161 cgccataata gatgatcgga attccgggaa ttgtcatgtt aaaagcctgc aacattttca
     8221 gtcttgcata acctatcgta tcagttattg taatatgacg gttccaaccg gcttcttttg
     8281 cattttcatt ccacttcaga tctccgctgg cataactgat aaatcgagcc atgtcatgat
     8341 tgccgctgat atttcccatc acatgatgcc aaccgaaata atcaaaactt tctttcaagg
     8401 atgatgacag gcgactaaat ggaatatcac cggaagcaaa tactgctctt gcatcaaaat
     8461 aaagatcgaa atcaaattgt gcatctaaaa gcccgctacc aatataactg ccaattaatt
     8521 ctctggaacc aaaagtttct ccgatctgat aaatcggttt ttgttgcgga atggcaactt
     8581 ctgtttttat tttaaatgtc aataatctcc agaattccgt ttcaatatgt ttagtggcat
     8641 catgtctgaa accatcaata ccaaatgtag ttaaccaata catcgcgcta tctgcacaag
     8701 cctccgccac tgcgggttgc gaatggtcga aacttggcat atatggctca aaccaggttg
     8761 tcaaccgatt ttcatcccac atccttaaat ttttcctgcc atccggaaga tccaattgcg
     8821 agaaccattc tggatgcgaa gtccacaagg gatgttctgc atgaatatga ttagccacat
     8881 aatcgagtaa gatattcatg tcgttgccgt gtgcatcgtc taccaaagat ttgaattcag
     8941 catttgtgcc gaagcgaaca tccactttac ttaatgaaat aggccaataa ccatgatacc
     9001 cgctaaacca tcgatgtggt tccggaaatt cctgataagc acccatcgga ttttgaaata
     9061 caggtgatat ccaaagtgta tttacgccca atttctcaaa atatccatca tcaatttttt
     9121 gcttaactcc cgccagatca cctccctgat agttcgcctg aggcattaca cgagcatcat
     9181 caacaggatg atcatttgat ttatcaccat ttacaaacct gtcgaccatc ataaaataca
     9241 taatggtttt ttggaaatca gtgcgatcca gcaatttcga atcagtaatc acttttccat
     9301 attccaatgg tatcaataaa tcattggaga tcaaattacc cctgtaggca taaattctga
     9361 tttgcgatcg ttttacagat ttcgcttgct ccggaacggc aatcacaata tccttacctt
     9421 cagaaaaatc ttcttcatgc tggctatata tttctttata ggtcagttga tggttatccc
     9481 aaaatacaac ataatgttct gcactgccag atacatccaa cgtgttcgtc aacgaatctt
     9541 cccacactgt atagatatca ggtgcatcac cttcgctgcc gccaacatta agcaatgaat
     9601 tatatccgcc aattccatta tcaatagaat ccttatttcc gggatctaaa aaccaattgc
     9661 catcagcaac aatctgatat tgatatcttc ccggatttag ttgcagcggt aatacccatt
     9721 tcccgtttac catttgcatg tgcaaaacat tcggattcca atttgtcaga tcacccacaa
     9781 tatttacctg cgtatatttt ttaccattgg ggtcgaagga aatcgatacc ggaatttttc
     9841 tcggcttttt taacaagaca tcaaacatct cattaccggc agagaaatgc aaggtactga
     9901 gtgtttgcat ttctcttccg ttgcgaatta acaacaatgt gtctttgtta tcagataatc
     9961 gtgcctgcaa aaaatcagga gttgtaacat tacttatcat cgacggctga agaatataat
    10021 cacgcgtgat gaccattgtt gtatcacctg aaagtgtaat cggctgcatc aaaccaaaac
    10081 tcagtgtttg tgatgctgcg ggcaacaata tgcttgttaa tacaaaaagt atacctatct
    10141 gtaaaaatga aaacctcaaa cgactgagca tgatgatatg atatcataca aaattagtgc
    10201 taaaatccat gagttagggc tgatcacaaa caaaatgcaa tcaaacaatt gatgaaaaca
    10261 gcctacaggc cagattgata ttactcccaa aatttatcaa ggtccatatc aaccatctgc
    10321 ggcatggtaa ctgttaattc cggtgttctg tgcatttcac ggtcgatggc aaacattgct
    10381 tctttgttgc gcgcccagct tcttctggcg atgccattat ttacatccca aaacaacatc
    10441 atttgcaaat tgcgatctgc tgcttcgcta ccatcaagca ccattccaaa gccaccgttt
    10501 attacttcac cccaaccaac accgccacca ttgtgaatac tcacccaggt tgcgccgcga
    10561 aagctatcgc cgatgacatt atgaatagcc atatccgcag taaacctgct gccatcataa
    10621 atattactcg tttctctgta tggcgaatca gtgccgctta catcgtgatg atcgcgacca
    10681 agaacaacag gtgccgataa ttttccttcg cgaacagctt tattaaatgc ctgtgcaatt
    10741 tttattcttc cttcagcatc agcatataaa atgcgagcct ggctgccgac aaccatttta
    10801 ttttccattg cagattttat ccaattgata ttatcgctca tctgcaattt aatttctgca
    10861 ggcgcatcgg catatatggt ttccatcacc tctgccgcta tcatgtcggt caatcgcaaa
    10921 tctttttcgt cgttgctgca gcatacccat ctgaaaggac caaagccata atcaaaacac
    10981 attggcccca gaatatcctg cacataactt ttatacttga aatcgccgtt aggcaacatg
    11041 atgtcggcac ctgctctcga tgcttccaat aaaaaggcat ttccataatc aaaaaaatac
    11101 atgccttgag cagttaattt atttatagcg gcaacatggc gacgtaaact ttcatatacc
    11161 ttttgtttat ataactcagg ctcttccgcc atcatgcgtt tgctttcctc aaaagataaa
    11221 cccgccggat aataaccgcc ggcaaatgga ttatgcaatg aagtctgatc gctgccaatt
    11281 tcgatttgaa tattatcatt ggcaagtttc tcccagagat ccacaacatt tccttgataa
    11341 gcaagcgaaa ctgcttcctt attttttctc gcctcctgca ttctggaaat aagtgtatcc
    11401 agatcagtaa atacttcatc cacccaacct tgtgcatgtc gttttgttgt tgcttcaggg
    11461 tttacttctg caacaacaca cactcctttt gcaattacag ttgcctttgg ttgtgcgccg
    11521 ctcattcctc ccaatcctgc ggtaacaaaa acttttcctg cacaatcctg cgcatgagta
    11581 tcgatttttc ttgccgcatt caacacggtg atattagtac catgcacaat tccctgaggt
    11641 ccgatataca taaatgaacc tgctgtcatc tgaccatatt gtgttacacc gagtgcatta
    11701 aatttttccc aatcatccgg tttagaataa tttggtatca tcataccatt tgtaaccaca
    11761 acgcgcggtg catttttatg tgaaggaaac aatcccatcg ggtgaccgct atataacacc
    11821 aatgtttgtt catcagtcat ggtggcgagg tatttcatgg caagcagata ttgcgcccaa
    11881 ttttgaaata ctgccccatt tccaccgtag gtaatcaatt cgtgcggatg ttgcgctact
    11941 gcatcatcga gattattttg caacatgagc atgatgcatg cagcctgaac actattatgc
    12001 ggatagtctt tataatgtct tgccttgatc acatactccg gcctgaagcg atacatatag
    12061 atgcgtccgt aggtcttcag ctcctcggca aactccgggg caaggtcggc atgcatatgt
    12121 ttgggaaaat atcgaagtgc gttgcgcagg gcgagcgatt tctcctcctt ggtcaggata
    12181 tccttgcgct tgggtgcatg gttaatgccc tgctgataag gctttgcggc cggtattgcg
    12241 gaaggaattc cattcaaaat ctgttccctg aactggcctg taagatctgc tgattggctc
    12301 atgaatgctg acttgtaagg gcaaaattac caattgccaa cccaaaaacc gacacttggc
    12361 acgatgttgg ttttaagggt agttgtaaat caaccgctta tgaaaaagtt attactcgct
    12421 acaacagtat ttgcattcgg attggtagcc tgcgacagaa acgagatacc tgattacgat
    12481 gtaagagatg aatatgttgg tatgtatgat gttaccgacg cctgcgatgc catgttaaac
    12541 gattatacat tggaggtata taagggttct tcctacgatg aaattgtttt cggttggccc
    12601 ggattgtatg aagcaggaat ggatgttacc ggaatagtaa ccggaatgaa agtggtaatt
    12661 cccattcaac aatttactat ttcaacctac ccgaatatat tttatgagtt cagtggtagt
    12721 gcttcattgg aagacagtat tctgactgtc gactatactg tattgactgt tcaggatgga
    12781 ctcattattg atgagcataa ctgtatagca actatggagc gcaattaatg caccacctgc
    12841 aaagatgctg tataagtagt atgttgatcg acagcatata tccaataagt acccgcagat
    12901 aattttgctg cattgacaat atgcattcca ttgcgatatt cagcaatacc gcaaagcatg
    12961 ccctggttat tgtaaatctg aaccgaaagg ttttcaggta atccaataat cagggcattg
    13021 tctgtggcag gatttggata tgtgagcacc tctacctgtc ctattgtatt tacggctaca
    13081 gcactgcgat cgaatttaac caatccacct tcttcagttc caatccatat aatatttgcc
    13141 gaatcaatag ctaaggcatt tataaaatca tcaggtaaat cgctgtttgc cgtggtatag
    13201 tgtgtccagc tgatattatt gaatatctcc aagccggtag cagacatacc tattacaccg
    13261 aggttattat tatccagacg cactgcatcc acactccaat cgctgatatc actggtaagt
    13321 ggtgtgaatt tgagaaaatc gcctgcttcc gtgaatacac tcaatccccc aaaagatgtt
    13381 gccagccatt tattatgata aatatcctca tcaatactca aaatggtatt gtcgctaata
    13441 gttgaattcg ccgtgcgata aattgtaaac acaccgtgat catacacatt taaaccgcca
    13501 tttaatgtgc cgatataaac cacatcattt gaatcgatgt atagggcatt gatattcggc
    13561 acaagtatat ctgaatttaa ttcagtgtag gtataccaat aatccgcgcc atcccatttt
    13621 gcaagtcccc atgaagttcc aatccacata gtatcatttg catcttttgc aattgactta
    13681 atatgataat ccggtaggcc tgaattggta gggtcataag ctacccaatc agtaccgtca
    13741 tatgacgcta atccatccag ataagtacca acccatacca catcattttc atcaataaat
    13801 attgatctta tctgattatc aggtataccg ctgttggctg tagtaaatgt tgtccatgtg
    13861 ccgaccggat caattttcac caagccgcct tcagtaccca cccaggtatt attgtgatca
    13921 tcaatggtaa tggcatttac caggtttgtt ggcagatctg aattatcctg attataaatt
    13981 gtgaatccgg attgcgcaaa gccgattgtt cctgcaatca acaaaaccat ggtgacgaaa
    14041 actctcatgt tacaaaatta gaggcttatt tgagatattc ttatttctag atggtgactt
    14101 tagtcaaacg aaactcattt taacacaaca aaaaaggctg tcacttaaca tgacagcctt
    14161 ttatatatta gcatttccga ttaatccaga tccaacagct ccattgaagc cttagcctta
    14221 ccatgtgtat cggaaacttt gtgtttgtaa gtaaatccaa acaatgggaa aacccatgct
    14281 ttaaagcgct cattcaataa atacattaca ggaacaacca ataatgtaat gaaggtggca
    14341 aagccgagac caaatacgat agtccaagct aaaggtcccc agaacacaac gttctcacca
    14401 ccgagccaga tatcaggttt accatgagta aataatccca cgaaatcgat attaaagcct
    14461 atcgccaatg gaatcatacc caatatggtg gcagtagcag tgagcactac cggtgtcata
    14521 cgaatacgcg ctgcttcaac aacagcctca cgcagtggca caccttcagc gatcaagata
    14581 tccgtaaatt caaccagcag gataccattc cgaacaacga taccaagcaa ggctacaatg
    14641 cccacaccgg tcattacggc agagaagtcc attccaaata aaccatatcc caaaagcaca
    14701 ccgatgatac taaataatac ctcagcgaag atgatgatag gtttagaaat ggagttgaat
    14761 tgcgctacaa gaataattac gataaggaat aatgaaatca accatgctac tccgaggaag
    14821 ctgaaagttt cttccatatc ctggctggca ccggtatagc cgattgttac accttccgga
    14881 gtatcaaaac cttccaacgc tgctttaatt tgattcatga taaccggttg ctgactttca
    14941 tatcctgtta aaagatttga agagacagtt accacacgtt tctgatcaat gcgtttgatg
    15001 gcagaataag tagatgcata atctatggta gcaaatgcac tcaatggaat ttggcgtatc
    15061 tgcccggtag tcatatcgcg ataagtgatc ttaagatctt tcaactgatc gatattctta
    15121 cgttgcgatt cgtcatagcg cacgatgatt ggaaaatcgt cgttaccatc tttaaatttc
    15181 gaaacttccc atccataaac ggcatttcta atttcaccac ctacggttag tgtagaaata
    15241 ccttctctgt tcatgcgttc gcgatcgagg tcgataacaa tttcaggttt atttgcctga
    15301 aggtctgttt tcaattcctc aatacctgca aagttcaaac tatcgagata gcgttttact
    15361 aattcactcg tttttatcag atcatcgaaa ttctcgccgg taagttcaat gttaattggt
    15421 ttacctgccg gaggaccatt ctgttcaaca ccaacagtaa tatcggcacc cggaatatct
    15481 tttacagctt cgcggatttt ttcgatatat ggttcggctt tttgatgtct gcgttctgaa
    15541 ggatcaataa acgcaatacc aacttttcct ttattggagg cagcgctctg atcaaatgga
    15601 tcttcacttg caccaatggt aacgttgctg atcactgatt tcaccaaagg attgtcttta
    15661 ccaatcactt cataaattct tttctcaaca atctgtgtaa ctgagtcagt agtttgctga
    15721 tcagtacctg ttggcattac cacatatgta taaataaagt ttggatcgcc tgaagggaat
    15781 tgctcaacac ccggttttgt aacaacaatt agtgcaattg caagaattaa tgttcccaat
    15841 attccaagga atatttttcc cggatgattt agtgtccaac gcaaaatact tgcatagcga
    15901 tgttggaatg caggccatcc ttttttctgg aaccaacgca cagcatgtat caatacaaga
    15961 ttatagaacc agaaaacagc aaccaatgaa atgacaatat ttccgaacaa cattttacct
    16021 gaagcatcga agagcaaacc aactgctatt aatatggcac tccaaattaa atgtctgcgg
    16081 cgatttggtt tttcatgatc atgatgcggt ttcatgaatg ctgcggcaaa cacagggttg
    16141 atcaaatacg ctacgatcaa tgaagctgta agtgtaatga tcaatgtgat tggcaaacct
    16201 ttcataaatg aaccgatcat acccggccag aacaacaatg ggaagaatgg agctaaggtt
    16261 gtcatggtac ctgccaatac cggaaggaat acttcacctg cggcattttt cgctgctgtt
    16321 ttgataggta ctttaccatt ggcgaatatc cggtgcgtat tttctatcac cacaatggcg
    16381 tcatccacca caattcccaa cccaagcagg aagccgaata atacgattac gttcattgac
    16441 attccgagta agctcagcac cacaaatgct atgaacattg acaacggaac agataaacca
    16501 acaaacatcg catcggttgc gcccatgaag aacatcaaca cgatggttac caacaggaag
    16561 ccgataataa tggtattgat aaggtcggca acctgtgttc ttgttgtagt agaggtatca
    16621 ccggtgatgg taatatttac atcgccggga atatcttttg cttttacatc ttctaataat
    16681 gtattgatat tatctgatgc ttcgatcagg ttctcaccac tctttttaat tacgttgagg
    16741 gtgataactt ttttattatc caaacgcgca aagctttcct gttctttgaa gtcgtcttta
    16801 atttgagcga catctttcag atagatagga taattatttg cattgttgat ggtgaggttt
    16861 tgaatctgtt caattttatc aaattcaccg ctcactgtaa tactgcgacg catgttttcc
    16921 actttcatgg taccgcctga gataatgcgg ttttcgttgc tgatagcggc atacacatca
    16981 tacatggtca attgtgcggc ttgcattttg tacatgtcga cattgatctg tatttcacgt
    17041 tcaggagcac cgatgatatc tacgcgagat atctgtggta actcttcaat tttatcttgc
    17101 agtgattcgg catatttttt caatgaacca agatcataat caccactcac gtttacctgc
    17161 atgatgggaa tttcgctgaa ttccactttc ataacgtttg gaccgaaagt tggatccagg
    17221 tcagaaggta aacgcggctt tgctttatca acggcgtctt ttactttttg attggcgtcg
    17281 ttgatatcgg ttcctgaaat aaattcaacc tgaatacttg aaaagtcctg tacggaatta
    17341 ctggtgattt tatttacacc actaattgca gcaatatctt tttcaagctc tttggtgatc
    17401 agattctcca tatcggatgg agaagatccc ggatagattg tcgacacgta aactgtcggc
    17461 atagtgatat ccggaaaaag ttccttcggc aatgcgttat aggtagagat acccatgaac
    17521 gtgaggatca ccgtaatcac aaatatggtg gttcggttat cgatcgccca gctcgaaggt
    17581 ttaaattcct tgaatatatc tttcatagaa gtatatggtt ttgagaattg gaattttatt
    17641 tattgatgca tatcattcat ttcgaattac atcatcgatt cctgttctct ctatgttaaa
    17701 atttaatttc ttgtccgtcg atcaggtttt ggtatcctga ggttaccacg cgatctcctt
    17761 tttggattcc ggaaacaatt tcagcatggc catcgtatgt ttttccaact gtgatctgac
    17821 ggcgctgtgc aattttcttt ccttctttat cggcagctac gtatatgaag ctcgcatcgc
    17881 ttgaatgctg aatcatattt aaatccacaa ccattgcttc cttcgcttca tagtcggcaa
    17941 tcttaataac tgctatcata ttcggcttaa gatcagactc acctgtaatg cgtgcttcaa
    18001 cgctgaaagt gcggctttgc ggattaataa cgcgtgctgc aaatgatact ttcgtatcga
    18061 tctctttgtt gaggtcggtg atgataattt tagcattatc tcctgttgac accacatttg
    18121 aatgtgattc agaaacttca cctttagcct tcaggctgct catattcacc acgcgaatac
    18181 cctgaaatcc gggagaaccg atctgacctg cttttaaatc aacattatct actaaaccgc
    18241 tgattggtga tgtaagcttg ctcatattta attgttctct agcagcggca atactttttt
    18301 ccaatgtttc aacattattt tttgctgaaa ggtattgcac ttcacttccg attttttgat
    18361 cccataatgc cttttgttta ttgtacatct ccttagcaaa tccgagttgc gattcgagtg
    18421 tttcaatatt tttgcgcatg gcatcattat ctaattcggc cagtaattgt cctttagaaa
    18481 cgctttgtcc tgctactaca taaactgctt taataacacc gggcatttca ggagtaacta
    18541 aaacgttttc atcagcatcg atcttacctt gaatttcgat atagtgcgtg aattcttgcg
    18601 gttgaatttc cgttactaca accagttcag gatcttttga ttttgtggta tccgtttttg
    18661 cgatttctgc ctccagctct ttaatttgtt tatccagtgc tgtacgttct tttttcaatg
    18721 cttccagctc agcttttttg tcgcccgact cttttggtga gcaggccgca ataaagagtg
    18781 ccattgcgag atatatgaag ttctttttca taaatattgg gttttaatag ttgcctaatg
    18841 ctttgttaag agatgtttgt gtattcaata cattgaatag tgcaccgata tagcttcctt
    18901 gtgcttggta cagtgtgctt tctgcggtac ttaattcgag gcttgatcca acaccttcgc
    18961 gatatttgat caaagccgta tcatatattt tttgtgccag tgcgatgttt ttcttctgca
    19021 cttcaaggtc atttgcggca ttcatataat ctgtttttgc cttttctact tcaagcaaaa
    19081 taccttgtgt agcagtaaac tgatcgagtt ttgctttttc atatgccacc tgtgccattt
    19141 gcacatttga gcggcgctgc atacccgaaa aaacaggaac tgaaagcgtg agtcctacaa
    19201 gaccggtttc aaaccactga ccatcgccac ttaaaaagtc gaattcattg cgttgtgcat
    19261 tttgtgaata tgaaccatac aacaccaatg agggtaaata catcgcttta ttcactttta
    19321 tatttaatgc atttaattgc accagacttt ccataacagc gaactcaggg cgagccaaat
    19381 aattgcttcc ggccatattc acttccttaa ttggcgccaa tgtatctgtg agttcaattg
    19441 cagtggacat atcaagtccc atgctgaatt tcaaatactg tgcggcgagt gcggtctgac
    19501 gttttgcatt gtcgagttga ccttgcaatg tggttttgct gatagtaaga cgatctacat
    19561 caatcccttc aacaaatccc tgcttgttca attcggtaac ttcgaataat accttatcga
    19621 ggttgctgat atttttttga agcagctttt cgaattcctg cgcaacgagg accgagttgt
    19681 aagcagattc aacattttta tggacatcag agagtgcaat ttttgattgt gtttgcacca
    19741 atgccgcata ttgttttgct gcgcttacgc caacaagcca tgcaccgtta aataataatt
    19801 gcgatgcgga gatacccgca gtcatattct gttctgttcc gaattgcaca ggtataaatg
    19861 ttccgggttc accgccaaaa aattcgcccg ggataagaga tgtggggagt acaaagaaat
    19921 tttggtaatc gagtttcgca tctacctgag gtaatcctga cgcaatacga ttgtcaacgg
    19981 tgatttctgc attctgcaca tccagctctg cagatttaat ctgcggattg ttcttcaggc
    20041 catagtcgat tgcttcctgc aacgaaaaac gcatagtttg cgcttgtaat attgccggaa
    20101 ggaggagtac ggccatgcat aacactttga atttatatgc catggttttg tgatttgagt
    20161 tgttctaaat attttatgcc ttttggcgtt gctattccgc gtaagtgata atcaatgaac
    20221 acgttaaatg ttcgttcagg tgaaaattta tctaaaggga aaatttcatt atcgataaac
    20281 atttcaacgc gtgtaatata aatacgagct atcacttcaa tatcgagatc tgcgcgatat
    20341 aaaccttcat ttatgcccct ctccagatta tgtcggatgc tgttgagcac ttcattattt
    20401 cggtgatcga taaatacctg ccagctttcc ggatgatatt ttctcaaatc atataacaag
    20461 gcaggattga ggtttttact catcgcattc atccaggctg caatcgaata catttcgtcg
    20521 atggcagttt tagagtgatg aatcacttgt tgcaccattt cattctggca tttaaagtga
    20581 ttctcagtca ccgtttggat gagttgttcc ttgttctcga aatactggta aagggtcttc
    20641 ttacttacac caagttcgcg cgccacgtca tccatggtca cgcttttgat gccgtacttc
    20701 atgaagagac gttcacagcg attgagtatt ttttttactt cttcgttcat tggcggcgca
    20761 aagatctgac aggaaaccga ggaaaccaag aattgtttga aagtttccat agtttttatt
    20821 tgattttaac atttcagccc ttttcggagg caaaattgac ctcgaaaacc gggaaaacgc
    20881 caggcgtttc gacgaaaccg ccaaaaattg agatgaatgg gtaaaatgcg gggatggagc
    20941 ttaaaacagc ctatttacaa gggtttctgg ctgtatttca cttgtggtca ggtgcagaat
    21001 gccgggggaa agtccgggtt tgaggatccc aaacctgtct gataagccaa gaaattgtgc
    21061 gccgccatcg gtagcccatt ggagcagctc ctccgtggta agggaaggaa aagtatgggc
    21121 aatggtcttc aattcgtcga ggatggacaa gccatggttg gaggcaaggc tgtcggtgcc
    21181 gatggcaata tgcttgatat gttgaatgag ttcagggata tcggggaggc gatcttcgat
    21241 gaacaaattc gcattgggac aaaaacacca atacaaattg gcatgcagat tttcggccca
    21301 atcggcatcc caaacggtag caatagtatt atgcaccagg agcgttttca cgtccttatc
    21361 cataaaaggc agtattgact ccaatgaagt tttacctgtt ggctcgaaat gtgtgatcgg
    21421 aattcccagt tcgcgatata attttctgta tccaccggtt gcctgaacat aaaactcatt
    21481 ctcgtcattg ctctctagca tgtgaatgct tgttggcata cccatatcac gacaggcacg
    21541 ggtgatgcct tccaccaaca aggtgctgat agtatagggc gcatgaggtg caagggaggc
    21601 acttaaattt ctttccttcg ccaaagcaac aattgcattt ccatgttcaa tagccgttgt
    21661 tgctttctgc ggatcaagtg ccaacaattc aacaaaggtg tgataatata tgcggctatc
    21721 agctttaatg tctaaactat gcggagcatt gctgatatcg ccaacagcta cgataccatt
    21781 ttgccacata ctactatcag cttcttgcat ggctgaaata atatcctcca ttgcagtttc
    21841 acgttgcctg gtcacttcca tcagaaatgc aggcaaaccc gttttcattg gaaattttcc
    21901 caacaaatgg gatagctcga ggtgacaatg cgcgttaaca aatccgggac aaagtgcacc
    21961 atcaaaatgc tcgatcttga gtggatcgat atcagcttta gcaacaatac catctatacg
    22021 accatcatga tgcacaatga gcacatgctc acgaagtgga tttccgtagg aaggataaat
    22081 gacatcggcc gacaaaaaac gcatgcgaca aatgtacagc gttattttgt gatgaaagga
    22141 agaaagtata tagaggaaga gtagtttaac ataaggctat ctactctccc tctaaagtaa
    22201 aatgtcctaa ttcaatttaa agttaacacc aagacggatg ccgccacctt tagttatcag
    22261 cgactgcgta gtttgcgaga gatcttgttc tgcgccatcg atactaaacg atttaaggtc
    22321 gaaattttgc tggctgtatt caaattgcag aaacacaccg gcaacatcga agaaatacca
    22381 attgaagccg gtaaataatc caagggccgg gcttttatat ttatattgat aatttacacc
    22441 tggagtgagc gcatcttttt tattgatttc aagatttgtc aatcctgccg aaccaccgag
    22501 ataccaatta aatttatcgt tattgacagg atacaatctc gcatcaaatg caataatgga
    22561 tacgtgattg ccatcattag attgatctgc cgtatcttcc agataagatc ctatattaaa
    22621 tgcaacaccg gcagaaaacc aaggggcaaa actatattgc gcatctgcat taaacatact
    22681 ggtgacggcg ccgctggtat ctctgtctac tacaacacca aaaaatgttg tttcactcgc
    22741 tgtaccgtgt atcatagcat catagcccac ttgaaaacta agcgttccac tttcaagtga
    22801 ttgtgctttt accgttatgg tgcataatac catgcacgca ataattaatt ttttcatgta
    22861 aggtgtttaa gatgtaaaat tatggaagca aatgccaccg gggcaaactg ctatacagaa
    22921 gtattccgca acaataatgc ctgatgatgc aaaaagagga cagttatttg ttaaataata
    22981 gcaatgagtc cccaataagc gaatgctatt gcacatagca accaaacaac agcattgaca
    23041 taaaacccaa ccctggcgcc ggttgtagtg accacttgct tgcgaaaaac cagccatcct
    23101 gtagctgcca tcactatcaa tgttaaaaca ggcatgtaag aaagcaaatt gatccaccca
    23161 cgaccggctt cgaaatatgg agcggggaaa taatacacag caggttcatt catgatcacc
    23221 caaatatata tcgccaacag tgcattaaaa atggcaatac taatgcgcaa atactgaaga
    23281 tttttttcag caagatgtaa ttgtctgaac cattgtgtcg ataaaattgc tgccatgccg
    23341 agcaataaaa taacaacaat gagcatgatt ggcaataggg tgatatgatt tgcgggcgtg
    23401 atgtatgatt ttaatgtttc aaattcctca ttcttgggga atagagtttc catggcaatc
    23461 cagtgttcac cttttgccaa ttcatcattc aatgagagaa tatgcacgcg atcataattg
    23521 tgataaaagc acaatgaaat taatcggcgt ttggtatcat acaatgaagt atacagggtg
    23581 ccatc
//
LOCUS       DAOQHY010000033        23435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_961_length_23435_cov_5.937682, whole genome shotgun sequence.
ACCESSION   DAOQHY010000033 DAOQHY010000000
VERSION     DAOQHY010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 23435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23435
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_961_length_23435_cov_5.937682"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3350
                     /locus_tag="PLL28_04405"
     CDS             <1..3350
                     /locus_tag="PLL28_04405"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNF68593.1"
                     /translation="IGDVNNDDLPDLFFTGNLVPSRLFINKGNLKFEDYTAKAGITSN
                     KDKWPYGASMVDFDGDNDLDIYVCMSRWDQPERRANEMWVNNGDGTFTEKAKDLGLTD
                     NSYSTHANFFDYDNDGDLDIYLVTHPVDFIDKNKTKYFQKIEQGKNLSNHFYINNGDG
                     TYTESHLKVGINNHGYGLSASVGDVNDDGYQDVFVSNDYAMYDYLYINNGDGTFRDES
                     LTAIKKTSINSMGTDISDFNNDGFLDMFVVDMDMEDNYTYKTFMLSSQVEVMRILINA
                     GYGYQNRSNSLQLNNGDGTFSEISRTANVSTTDWSWTTFFTDFDNDGWKDLFIANGFL
                     RDFHVDESETYHKLRRAVRIEDSTVYYEVRKELPNYVLDHPNYIYRNNGDLTFEDKRD
                     DWGIYYPSITYGGGYADLDRDGDIDMITSNVNQSPWVYRNNSEKMNTGNNYINFKVGG
                     YAKNTAGLGTKVRIFTGDNMQYIQYTASRGYITTIIDNIHFGVGKATKIDRVEVEWLD
                     GTKLMLTDVPVNQVVKLDHAQASKSVQFPVRVKGDKIFANNTESLGTDFEHKEADYDD
                     FLREFMLPHKMSTLGSGIAVGDVNGDGTDDFYIGGAAGQSGALYTQNENGKFTRSSWT
                     QGMNDGLASEDAGALFFDADGDGDNDLYVASGSNEFASNDKRYADRFYRNDGKGNFTY
                     DSSVLPAINSSSSSVSGCDFDKDGDIDLFIGGRQVPGRYLAPASSYILKNDGGKFSDA
                     TAQVAPMLKDIGMVTSGLWTDFNNDNNIDLILTGDWMSITFLKNSGGKFTDVTASTGL
                     NNATGWWQSITGADLDNDGDMDYVVGNFGTNRRYKNTVSSQGKPLPLEAFLYDFDKSG
                     QEDYIMCYYQHDKLFPVKTRERMIEQIPSFEEKFPTWNEYGIATVQDMFGKDLDNAIH
                     RTAYIFHSSVLMNDGNGKFTMKFLPNEAQISTMFGTIIDDFNEDGIQDILMHGNFYNT
                     EIEITRHDAGTGLLLIGKGDGTFTPNRSYINGFKSDGDAKALATLLDSKTKQPVILAG
                     NSQGKMLAFSLVKPTKTIELAAADAYAIVTRTDGKTTRVELYSGSGYLSQSAKSIRIT
                     PLVKQVDVYDYKGNMRTVYPGGNLASK"
     gene            complement(3429..4067)
                     /locus_tag="PLL28_04410"
     CDS             complement(3429..4067)
                     /locus_tag="PLL28_04410"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNF68594.1"
                     /translation="MQKFLPIFPLNLVAFPGEQLNLHIFEPRYKQLISECSKENKTFG
                     IPVVDNNQMLDHGTEMELIKIHKVYPNDEMDIQVRGLQVFRLLEVVREIPDKLYAGAI
                     ISLLPNVEDKHSRLALELEELTVELFSLLDVREQMEKPDFAFKSFRLGHYVGFDLKDE
                     YELLRHVRETERQKLIVEHIKRILPSVKQVSDMRERAKLNGQYRMVNPPDFL"
     gene            complement(4076..4699)
                     /locus_tag="PLL28_04415"
     CDS             complement(4076..4699)
                     /locus_tag="PLL28_04415"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HNF68595.1"
                     /translation="MGSRFYFSLMGCVLILFASSCRQKQTTLPDPHLSYAQVIQKQRN
                     DQQAYFAGEGSPLSDSARANFKGLQFYPIDSSYRVSGNFSPIANGPIFTMQATGDVAD
                     IYQIAGNIQFKLKDTICMLEVYRNVTYYQVEHKESYFVPFYDKTNGQETYEGGRYLDV
                     HKLQPGAMQLDFNLAYQPYCFYSHNYSCPIPPRVNSLPVYVRAGERK"
     gene            4752..5123
                     /locus_tag="PLL28_04420"
     CDS             4752..5123
                     /locus_tag="PLL28_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HNF68596.1"
                     /translation="MASLFDIEAFRDYCLSKKGVTEEFPFGPDTLVYKVMGKMFALSG
                     LDEIGFSMNLKYNTEHIDELRDKYPAVQPGYHMHKRHWNTVMVDGSIPVKELKSLIDH
                     SYSLVVDSLPKKLQAELNALK"
     gene            5124..5819
                     /locus_tag="PLL28_04425"
     CDS             5124..5819
                     /locus_tag="PLL28_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HNF68597.1"
                     /translation="MISEDQILYIDNHLIAINKKPGEISQSDVTGDVSLLEDVKLFIK
                     EKYNKPGNVYVGLVHRIDRPVSGVLLFARTSKAAERLSQMIRDRAFKKTYWAVVKQMP
                     KEPEGRLENYLLKNAQMNKSFVAKNEKGGAKKAVLHYRLIYSLERYHLLEVILETGRH
                     HQIRAQLSAIGCPIKGDLKYGFDRSNPDGSIHLHARSISFEHPVTHAAITIIAPPPQD
                     VIWNACLKVAEQQ"
     gene            complement(5867..6706)
                     /locus_tag="PLL28_04430"
     CDS             complement(5867..6706)
                     /locus_tag="PLL28_04430"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNF68598.1"
                     /translation="MKKATLLIIAFLGIAGVASAQQGLHFGFHAQMNSIWIINQNNYE
                     YSQMDYEYKYGKLGGMSLGYNWDDNFGWQVELNYSQMGQDYSDIIKDFGPITDSTKPD
                     LRTKVLTYRYVDLTYLQVPVLFKYMEGDSKDAIKYQMLGGLQFGYLLGADQQYTADLY
                     DLDPDDQKPVEQAGFHAPFDAVPDFSGNAGSDGTEFFTKLDVGALIGIGADIYVNDKL
                     YFTPSFRGYFGLFDINAKETRELRPAQGENRYLASHNAFVGFHLGISFMFPDARLGGG
                     SDE"
     gene            6965..7753
                     /locus_tag="PLL28_04435"
     CDS             6965..7753
                     /locus_tag="PLL28_04435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF68599.1"
                     /translation="MFNVHMAGQFLRYQVRALSRYELHSPFLFDLEEKVLHDKRHFYA
                     FDEIEYRRELLLAEDQTISVSDFGAGSHVHKSPERRVSDIAKTSLITPHFGQMLYRLV
                     QYLQPVTLLEMGTSLGISSLYMAKAVPGAQLITLEGSSAIAQIARKQHIALDAKNITI
                     MEGEFNATLPSALNALKKIDLAFIDGNHRFEPTVAYFESIYPYLHEHSILVFDDIHWS
                     TGMDKAWHVIKSDARVTCSIDLFYKGIIAFRPEFRQPQHIVLRY"
     gene            7836..9209
                     /locus_tag="PLL28_04440"
     CDS             7836..9209
                     /locus_tag="PLL28_04440"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNF68600.1"
                     /translation="MRKRMMISMCLLLLAGIMHAQSPLPSNFFLKTLPNGLQMLVIED
                     PTVPLATIEIVVKNGSYTEDSTFNGLSHMYEHMFFKANKSLPSQEAFLEKVQELGVVF
                     NGTTSNERVNYFITLSNAKLGEGLVFMNNAIRYPLFLEQEMKNENPVVDGEFQRNESN
                     PVFFLFDDLNRHMWKSNYYRKNAIGLHDVILTCTPEKMRIIQEKYYYPDNSLIAIAGD
                     VKHDDVFAQVEKIYGDWKPGGFDPFQKWPIPEFEPLKGVENFVTINENAQIPMFITGY
                     HGPDTRNDVKATYAADVFSYLLNLSDSKLRQELVETGLAYDVNVGYQTCKYTGPINIF
                     LVPNPAKVSEAWKTLQENIGQWASPNYFTDEQLETAKNQLAIQDAYDKESISNYVHTV
                     TYWWASASLDYNTNYIKNVQAVTREDIQRYVETYIIDKPHVNGLLLHPDMLQMVDVKA
                     LGFEVKQ"
     gene            9237..10565
                     /locus_tag="PLL28_04445"
     CDS             9237..10565
                     /locus_tag="PLL28_04445"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNF68601.1"
                     /translation="MKKIQITIVLLVTLLSANRLTAQDTKEYMVDGIKVIHKQVPKDV
                     ISVQLFIEGGTANYTKDKQGIENLTLNLMMDGGTTGMSKLEFKSAAEKIGTTFNAGSN
                     IDAGTLSMNCIKLFWDDSWKLFSDAILHPAFGEAEYNLLREQLIAGAQQSMADPDTYL
                     ALLSRQVAFENKDYAKAPEGTPETLTGITLDDVKTYYKKTIGKKRVFLVVVGNISQED
                     LTAKIHNTLAALPGGSPASSESRTLITQPKTYIEDRDIATNYIRGIMSAPFANDAEGI
                     AYRVAISIVGDRYFTELRTKRSLSYAPAAFYANAGITNPYGVIYITTIDPKQSMQVMV
                     DELNKIKKEGFTEKELRDKQQEFLTTYYLTLETTANQSNALGMAEMSGGWENLDKITA
                     AVNSLKLEDINAVFDKYSNAIVWTYLGKKSAISEADFPQTTPKEKKNKPY"
     gene            complement(10634..11149)
                     /locus_tag="PLL28_04450"
     CDS             complement(10634..11149)
                     /locus_tag="PLL28_04450"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNF68602.1"
                     /translation="MASKRVSESATTFTEIVMPNDTNPMHNLMGGNLLKWMDIACGIC
                     ASKHANRVAVTAAVDNVSFGRPIKIGDIVTISARMTRAFNSSMEIFVEVFKENFRTHE
                     KVKCNEAYLTFVALDDDGRPVSVPEIEPENDDEKRRFVSALRRRELRLILAGRMKPEE
                     AKELKQIFLNE"
     gene            11309..12172
                     /gene="ygiD"
                     /locus_tag="PLL28_04455"
     CDS             11309..12172
                     /gene="ygiD"
                     /locus_tag="PLL28_04455"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HNF68603.1"
                     /translation="MERRKFIKAGTFSLLAMQLKEFSNTFQQEQDIVMPAMFVGHGNP
                     MNAIEDNEFSRGWEAQGKLLPVPKAILCISAHWLTRGTRVAAMEKPETIHDFGGFPQT
                     LFDVQYPAPGSPEFAKETSTVITKTKVELDFEWGLDHGCWSVLNKMFPNADIPVYQLS
                     IDYYQTPAYHYALAEELKSLRKKGVLIMGSGNIVHNLGMVQWADTAYDWAIEFDTKIK
                     EFIDARNHQGVINYKDLGKSALLSVPTNDHFLPLIYTLGVTDTKEAISYFNEKNTMGA
                     VSMRSFVAGLS"
     gene            complement(12226..12537)
                     /locus_tag="PLL28_04460"
     CDS             complement(12226..12537)
                     /locus_tag="PLL28_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68604.1"
                     /translation="MKRCIIILFVIAAMPVFGQSARISPAAFTSLSVGFVQHTEPVPH
                     GGQGIAATTVNPNPNVGTSNIVVQEELLAGRKSNPGLREKITSRERNTNKQPLFKKKR
                     H"
     gene            12624..13934
                     /gene="sucC"
                     /locus_tag="PLL28_04465"
     CDS             12624..13934
                     /gene="sucC"
                     /locus_tag="PLL28_04465"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNF68605.1"
                     /translation="MLITELAVNSCDVLITNTLAYFNRNEAKRIVYICPQNAKYMNLH
                     EYQGKELVKRFGVKIQEGIPVDTPEQAVEAARQLQAATGANIWVVKAQIHAGGRGKAG
                     GVKVAKSLDEVYEKASKILGATLITPQTGANGKKVHKVLVAADVYYPGASDTKEFYMS
                     ILLDREKSRNVIIYSTEGGMDIEEVAEHHPDKLHKEWVDPTVGIQAFQARKIAFNLGL
                     SGAAFKDMVKFVTSLYNAYVGNDCSLLEINPVLKTSDDQIIAVDTKCVVDGNALYRHA
                     DIMGMRDITEEDPTEVEAGEAGLNFVKLDGNVGCMVNGAGLAMATMDIIKASGGNPAN
                     FLDVGGTADAKRVETAFKIILKDPNVKAILVNIFGGIVRCDRVAQGVIDAYKNIGNIP
                     VPIIVRLQGTNAEEAKKMIDDSGLQVRSAILLKEAAALVKEAVL"
     gene            complement(14303..14569)
                     /locus_tag="PLL28_04470"
     CDS             complement(14303..14569)
                     /locus_tag="PLL28_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005827755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNF68606.1"
                     /translation="MVITFEELRHIKDALPHGSMERIAEELHIEVDAVRNYFGAEHFE
                     APGKIADVHFEKGISGGVVRLEDTAILQSAMRILDEAKHVQTLN"
     gene            14814..20210
                     /locus_tag="PLL28_04475"
     CDS             14814..20210
                     /locus_tag="PLL28_04475"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HNF68607.1"
                     /translation="MRAKLIWPGLLALGLYACNADRNTIAIKTGDFPETIELRQNLEI
                     HLNQHVFNDSLFDKWVDADLLQIDPPVEGRFKIVSSDAFYFSPSKGFAESEAYTATLG
                     KDLFNYTDLKLALKSAPVNFHTTLLDLTAVNTYWFLEPTSGEVKLNAGLQFTASVDPQ
                     TLGDMLTVKVDGTNKTASITSTQSADNMAVTISGISPKAKDQDLNIDIRAGIGSPGSR
                     YKTPKPLHMKVPVAPVEDLEIRDITANHDGIVGTITVESNQQFSNTNLEELVKIEPSI
                     PVTIEKTLNGLVVTSEQFNASQTYVIILSDKLRGVAGGKMVDTYQQSVTFGELEPSIE
                     FTQQKARYLGGKGERNISVRIVNVPKVQVNVVKIYENNILAFLRDGFSWDWYESYDAE
                     EEYWDYHDYQNVNTSLYGNVVYTEEIETSKLEKYNSARVLHLDFTDKLERFDGIYVVT
                     VSAAEKVWLTDAQIVSISDIGLIAKATEDELVVFANSIQTAEPMSGIKVSFISSNNQK
                     LYTASTDKNGVARFTGLKQNMPDFNVNLITAKNGSDYNYLPFSETEVSTSRFDVGGKR
                     LNSSDIDAFLYPERDLYRPGETMHIAGILRTYDWQLLDRIPVKIKVISPSGKDFKTLR
                     KTIDDQGGFETGIDIPKNAVTGTYTVQLFSSNDILLASEYISIEEFMPDRIKVSGSLN
                     KQNYYPGESVQSTIVANNLYGTPAAERNYESEFNLQQHYFSSKKYPAYNFQISGGTTY
                     FPSQNAEGQTDENGQAVQSYTIDQTYSKVGLLDGRILNTVFDESGRPVYQVQNFTVIT
                     QSNLLGIGEFDYYVKVKSPLRIPMIAVDKDDKGFKTQDARVEVIKYDYETVIEKHRNY
                     YSYRSNRKEKILQSYDKKISGDQDGIWFTPQQSGEYQIRLYLKDAKAYVEKSFYAYGW
                     GSTQNNSFEVNTEGNINIISDKPKYDLGEEAEFLFTTPFNGKLLVTVEREKVIEYHYL
                     DTEKKAASLKIKMNQSHVPNVFVTATLFRPNDESGMPLTVAHGIINVEVKNPAKAIPL
                     TVTHEDHSRANTKQTINIKTAPNAQVTVAVVDEGILQIKNFTTPDPYNWFYMQHALGV
                     FSYDLYAYLYPEIYLKKMLSGGDAMMNEGRLNPMTANRVKLVSYWSGILKADASGKIA
                     YTIDIPQFSGDLRVMVAAYRNDQFGASESHMKVSDPLVMTTSLPRFLTPGDTIDVPIT
                     LANTTDKKASGKVTMSIEGPIKVVGSSTQQFNANANKEARSVFKIVAWGATGNAKVKV
                     QSSALGETFTDITEIPVRPPAGLQKYSGEGYLDGNKSITVNMQPNLISSSAKARLMIS
                     YNPLVQFADDLDYLVQYPHGCVEQMTSSAFPQLYYADMVKSFYSKERPDLDPAYNVQE
                     TIRILQSMQMYNGALTYWPGGGYESWWGSIYAAHFLLEAKKAGYDVDQKTLDKLLGYL
                     RTMLKQKKTYVYYYDKGLSVEMAAQEVFYSMYVLAVSGKAEMSMMNYYKANLSMVPQD
                     GKYLLAMSYMLAGDKRSADKLLPGGFTDADPKQQFNGSFNSPIRDRAIALNCMLEADP
                     NNQQIGRLSQQLSEMVKNARYMNTQERAFTFLAFGKLAKQSKEHNAKVVIKIGGKEAG
                     TFDGTDLVIEDTKMASSPVTLNSSGSGRVYYFWYEEGVSADGSVKLEDNFLRVRKSFF
                     NRWGQPIDLKNIHQNDLVVVKISLVSATGTYVENIAITDILPAGFEIENDRLRATNSM
                     PWIQNQSEAQHEDIRDDRILLYTDATAKEKYFYYTVRAVTTGTFKMGPIMADAMYNEE
                     YHSYNGGGWVTIKK"
     gene            complement(20481..22040)
                     /locus_tag="PLL28_04480"
     CDS             complement(20481..22040)
                     /locus_tag="PLL28_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68608.1"
                     /translation="MKRLMLLSIFSVFSSLFVKAQSGDVNDLPPIDELGTGTFMGRQG
                     GLYPNGSNSMPAAFYADAMAMAQSVQPLDKSGKPDPKGRIGVIALGASTVAMFSKGLE
                     HQIPEAAGVNKELTFVNCGIGGQDLSDIMNPAANYWSVIDQRVREAGMDLNQIQVIWF
                     QEDNLRNRENNFDLRIAQLTKEFTYMVRFCKEHYPNVKLFYVSGRHTTDFMPADAKDK
                     HREPKAYINGWVCKQLIENQINGSEELAFKGANAVSPLILWGPYFWTQGEKPRKDGYT
                     LTRDMISNDGVHPTEKGIEKVSKDLVDFWRTDPVSQQWFLENPGEVTVDTIGDETAAD
                     MQTMAFIINSKTILNIPYDKIAATYKLTVLKDSAVVFNNDAAQVGPTLELDIAGAGNY
                     KYLISDGTNNYAGKVVVDDALQVTVVKDTVVSGKGKHPIDPNSPAWVVNGANKMAKLQ
                     HVLRPNTNVKVILTDDKGKEVLVVEDFLNKYTDVNEALDRGEYKMTFYNEKGSVISVP
                     EEFNSSIRVKY"
     gene            22195..23280
                     /locus_tag="PLL28_04485"
     CDS             22195..23280
                     /locus_tag="PLL28_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68609.1"
                     /translation="MLGYTDMQGDYYVSDSGRIVHLEHLRVKSSQPAANSIAYISNTG
                     NLVYYSNHEQVKLDITNPTFYKNTDHYLYYSTGGSFSVYNGKERKYLGYIQQYPYAFA
                     DSIAAVHDYSGYFYTYVNDLFIELENQPVLKVIAGDNILAYVNHIGQFKAYYQGNKVD
                     LDDYAPITAQAAGSIIAYIDNYQYMKVYYQGSIHELFNLPEINCLSTTSSGDPLLASW
                     CNGPVVTDVQSELPVFQVGDDMVAYIDDMGRFNVFYKGNIVALESQPPKQYTVVDRVL
                     YYTDENDYLKVFCNGELSIVETYTPSRIEADGEVLAYMDLDKRLKAFYLNTSVDVSEN
                     IVLDFSLNNTLLMYSEIPNKYKFYSLE"
BASE COUNT         7183 a         4789 c         4807 g         6656 t
ORIGIN      
        1 gtatcggcga tgtaaataat gatgacctgc cggatctctt ttttacaggt aatcttgttc
       61 caagtcgctt gtttatcaat aaaggcaact tgaaatttga agattatact gcaaaagcag
      121 gtatcaccag caataaagat aaatggcctt atggtgcatc tatggtcgac ttcgatggcg
      181 acaatgacct cgacatttac gtgtgtatga gtcgctggga tcaacctgaa agacgcgcaa
      241 acgaaatgtg ggtgaataat ggcgatggca cttttaccga aaaagcaaaa gaccttggcc
      301 ttaccgataa ctcctattca acacacgcca atttcttcga ttatgacaac gacggcgatt
      361 tggatattta tctggtaaca catccggttg actttatcga caaaaacaaa accaaatact
      421 ttcagaaaat tgagcaaggt aaaaacctga gtaaccattt ttatatcaat aatggtgacg
      481 gtacatatac cgaatctcat ttgaaagtcg gtatcaataa ccatggatat ggattgagtg
      541 cttccgttgg tgatgtgaat gacgatggat atcaggacgt attcgtttct aacgactacg
      601 ccatgtatga ctatctctac ataaacaacg gcgatggtac tttccgcgat gagtcgctca
      661 ctgctatcaa aaagacttcc atcaactcaa tgggaactga tatcagcgac tttaataatg
      721 atggtttcct cgatatgttt gttgttgaca tggacatgga agataactat acctataaaa
      781 ctttcatgct ttcaagtcaa gtggaagtga tgcgcattct tatcaatgcc ggttatggtt
      841 atcaaaaccg ctctaactca ttacagttga ataacggcga tggcactttc tcggaaatat
      901 ctcgtactgc aaatgtttct actaccgact ggagttggac aacattcttt acagatttcg
      961 ataacgatgg ctggaaagat cttttcatag ctaacggatt tcttcgcgat ttccatgtgg
     1021 atgaatcaga aacttatcac aaattacgcc gtgcagtgcg catcgaagat agtacagttt
     1081 attacgaagt gagaaaggaa cttcctaatt atgtactcga tcaccctaac tacatctatc
     1141 gaaataatgg tgatcttacc ttcgaagata aacgcgatga ttggggtatc tactaccctt
     1201 ccattactta cggtggaggt tatgccgatc ttgaccgcga tggtgatatc gatatgatta
     1261 caagtaacgt gaatcaatca ccttgggtat atcgcaataa cagcgagaaa atgaataccg
     1321 gcaataacta catcaacttc aaagttggtg gatatgcaaa aaatactgcc ggtttaggta
     1381 ctaaagtgcg cattttcacc ggcgataata tgcagtacat tcaatacact gcaagtcgcg
     1441 gttatatcac taccattatc gacaacatcc acttcggcgt tggcaaagca acaaaaatcg
     1501 atcgcgtgga agttgaatgg ttggatggta cgaaattgat gcttactgat gttccggtta
     1561 atcaggttgt aaaacttgat catgcacagg catctaaatc agtgcaattc cctgtgcgtg
     1621 tgaaaggcga taaaattttt gctaataaca ccgaatcatt aggtacagat ttcgaacata
     1681 aggaagctga ttacgacgac ttccttcgcg aattcatgtt gcctcataaa atgagcacac
     1741 tcggttccgg aatagctgtt ggtgatgtta atggcgatgg cactgacgat ttctatatcg
     1801 gtggtgcagc cggtcaatca ggtgcattat atacgcaaaa cgaaaatgga aaattcaccc
     1861 gctcaagctg gactcaaggc atgaatgacg gtctagcatc tgaagatgcc ggtgccttat
     1921 tctttgatgc cgatggcgat ggcgataatg acttgtatgt agcttcaggt agcaacgaat
     1981 ttgcttccaa cgataaacgc tatgcagata gattctatcg caatgatggc aagggcaatt
     2041 tcacttacga tagtagtgta ctgcctgcca ttaatagtag cagttcttct gtttcaggtt
     2101 gcgactttga taaagatggt gatatcgatc tatttattgg tggtcgccag gttcccggaa
     2161 gataccttgc acctgcttca agctatatcc ttaaaaatga tggtggtaaa ttctctgacg
     2221 ctaccgcgca ggtggcacca atgttgaagg atattggtat ggtaactagt ggtttgtgga
     2281 cagacttcaa caacgataat aatatcgacc tcattcttac aggcgattgg atgtctatta
     2341 ccttcctgaa aaattctggc ggtaaattca ccgatgttac cgcttcaaca ggattgaaca
     2401 atgctacagg atggtggcaa agtattaccg gtgccgatct ggataatgac ggtgatatgg
     2461 attatgtggt aggcaatttc ggaactaacc gacgctataa aaacaccgtt tcttcacaag
     2521 gaaaaccatt gccactggaa gcattcttat acgatttcga taagagcggt caggaagatt
     2581 atatcatgtg ctattatcag catgataaac ttttccctgt taaaacgcgt gagcgtatga
     2641 ttgaacagat tccttcattt gaagagaaat tcccaacatg gaatgaatat ggtatcgcta
     2701 ccgttcaaga tatgtttggc aaagatctgg ataatgccat tcatcgcacc gcttacatct
     2761 tccattcttc agttttaatg aatgatggaa atggtaagtt cacgatgaaa ttcctgccaa
     2821 acgaagcaca gatctctacc atgtttggta ctattatcga tgatttcaat gaagatggca
     2881 tacaggatat cctgatgcat ggtaacttct acaatactga aatcgagatt acacgtcacg
     2941 atgccggaac cggcctgttg ttgatcggaa agggtgatgg cacttttaca cctaatcgct
     3001 catatatcaa tggattcaag agcgatggcg atgcgaaagc attagctacc ctactcgatt
     3061 ctaaaaccaa acaaccggta attcttgcag gaaacagcca aggtaaaatg cttgcattca
     3121 gccttgtaaa accaacaaaa actatcgagc tggcagcagc agatgcttat gcaattgtga
     3181 ctcgcaccga cggcaaaacc actcgcgttg aattatacag tggaagcggc tatcttagtc
     3241 aaagcgctaa atcaatccgc ataacaccac tcgtaaaaca agttgatgtt tatgattata
     3301 aaggcaatat gcgcaccgtt tatcccggtg gcaaccttgc ctcgaaataa tttatatgaa
     3361 tcaaataaaa agccggtaat tagttaatta ccggcttttt ttatgtctat attttagtca
     3421 atatcaaatt acaaaaaatc aggtggattc accatgcggt attgcccatt caacttcgct
     3481 ctttcacgca tatctgacac ctgttttaca cttggcaaaa tgcgtttgat atgttctacg
     3541 atgagctttt gccgctcagt ttctcgcacg tgtcgcagca attcgtactc atctttaaga
     3601 tcgaaaccaa cgtaatgccc aagtcgaaat gatttaaatg caaaatcagg tttctccatc
     3661 tgctcgcgaa catctaaaag cgagaataat tctacagtca gctcctctaa ttccaatgcc
     3721 aacctgctgt gcttgtcttc tacattcggc aataaggaga tgattgcacc ggcatataat
     3781 ttgtcgggaa tttcgcgcac tacctccaac aaccgaaata cttgcaatcc ccgcacctga
     3841 atatccattt catcattcgg atataccttg tggatcttga ttaattccat ttcagtgcca
     3901 tggtcaagca tttgattgtt gtcaaccaca ggaatgccaa aagttttgtt ttccttactg
     3961 cattctgata tcaattgctt ataacgaggt tcaaatatgt gcagattgag ctgctcgccc
     4021 ggaaaggcaa caaggtttag tggaaatatg ggaaggaatt tttgcatcgt atttcttatt
     4081 tcctctcacc ggctcttaca taaaccggta aactatttac tcgtggcggt ataggacaag
     4141 aataattatg actataaaaa caataaggtt gataggctaa attaaaatct aattgcattg
     4201 cgccgggttg aagcttatgc acatcaagat accttccccc ttcataagtt tcctgtccat
     4261 ttgtcttatc ataaaacgga acaaaataag attccttatg ttcaacctga tagtaggtga
     4321 catttcgata tacttccagc atgcaaatag tgtccttcag tttaaattgg atattgccgg
     4381 caatctgata gatatcagcc acatcgccgg tagcttgcat ggtaaatatg ggaccattgg
     4441 cgataggcga gaaattccct gataccctat agcttgaatc aatggggtaa aattgcaaac
     4501 ccttgaaatt agctctggcg ctgtcgctca acggactccc ttcgcctgca aaatatgcct
     4561 gctgatcatt tctttgcttt tgaatgacct gcgcataact caaatgagga tcgggtaatg
     4621 ttgtttgctt ttgcctgcag gaagatgcaa acaatatcaa cacgcacccc attaacgaga
     4681 agtaaaatct gctgcccatc gtaggtcaaa tgtagaaaaa atctttactt tatgcatcca
     4741 tttaaaccgc catggcatcg ctgttcgata ttgaagcttt ccgcgactac tgtttgtcga
     4801 aaaaaggtgt taccgaagaa tttccttttg gtccagatac acttgtctac aaagtaatgg
     4861 gtaaaatgtt cgcattgtcg gggctcgatg aaataggttt ttcgatgaac ttgaaatata
     4921 atacagaaca tattgatgaa cttcgcgata aataccctgc tgttcaaccc ggctatcata
     4981 tgcataagcg acattggaat acagtaatgg ttgacgggag cattcctgtt aaagaattaa
     5041 aatcgttgat cgatcattcc tattcgcttg ttgttgatag ccttccgaaa aaattgcagg
     5101 cagaactgaa tgccttgaag taaatgataa gtgaagacca aatactatat atcgacaatc
     5161 atctgattgc catcaataaa aagccgggag agatctcgca atctgatgtt acaggcgatg
     5221 taagtttgct ggaggatgta aagctgttta tcaaagaaaa atataacaag cccgggaatg
     5281 tttacgtcgg actagttcat cgcattgacc gaccggtaag tggggttttg ctgtttgcac
     5341 gcacgagtaa agctgcagag cgactttcgc agatgatcag agacagggca tttaaaaaaa
     5401 cttattgggc ggtagtgaag caaatgccca aggagcctga aggccgattg gaaaattatt
     5461 tgttaaaaaa tgcacagatg aataaatcgt ttgtggcaaa aaatgaaaaa ggtggtgcca
     5521 aaaaagcagt gctgcactat cgattaattt actcacttga aagataccat ctacttgaag
     5581 taattttaga aacaggtcgt catcatcaaa ttcgcgcaca attatcggca attggctgtc
     5641 cgataaaggg cgatttgaaa tatggatttg acaggagtaa tccggatggt tccatacatc
     5701 tgcatgcgcg ttcaataagt tttgagcatc ctgttacaca tgctgcaatc accatcatag
     5761 ctcccccacc gcaggatgtc atctggaatg cctgcttgaa agttgcggaa caacaataaa
     5821 aaaacccgcc aaaagcgggt tcaaatattt taaagaagtt tattaattat tcatcagaac
     5881 caccgcccaa tcgagcatca gggaacataa agctgattcc gaggtggaaa cctacaaatg
     5941 cattgtgcga agcaagatat ctgttctcgc cttgtgcagg acgtaactca cgagtttcct
     6001 tcgcattgat atcaaacaat ccgaaataac cgcggaacga tggcgtgaaa tagagcttat
     6061 cattcacata aatgtctgca cctattccaa tcaaagcgcc aacatcaagt tttgtaaaaa
     6121 attctgtccc gtctgaaccc gcatttccgc taaaatcagg aacagcatca aatggagcat
     6181 gaaatcctgc ctgctctacc ggtttttgat catcaggatc caaatcatat aaatcagccg
     6241 tatactgctg gtctgcacca agtaaatagc caaattgcaa accacctaac atctgatatt
     6301 tgatcgcatc cttggaatca ccctccatgt atttaaacaa tacaggcact tgcaagtaag
     6361 tcaaatcaac atatctgtaa gtaagcactt ttgtgcgcaa atcaggcttt gttgaatcgg
     6421 taataggacc gaagtcttta atgatatcgg aataatcttg ccccatttga ctgtaattca
     6481 gttctacctg ccagccgaaa ttatcatccc agttatatcc cagcgacata ccgccgagct
     6541 tgccatactt atactcatag tccatttgtg agtattcgta attattctga ttaattatcc
     6601 agatgctgtt catctgtgca tggaaaccaa aatgcagtcc ctgctgtgcg gaggctacac
     6661 ctgctattcc caggaaggca atgatgagta gtgtcgcttt tttcatacca attggttgga
     6721 tcgttcgcag cgaatataca acctttgttt atttcactat gaaggcaatc aacaatttag
     6781 cctttttatg tatataaaac agttcgtgga gggtagtact catttgtcac atatttcctt
     6841 attttataat tcctatatta ctgtatatca atattttaaa tatttttttg tggatatatt
     6901 ttattgttta ccatgtcact gaatgtcaaa cctagaaaca aaatttaccc cgtatcttcg
     6961 ctccatgttc aacgtgcata tggccggtca gtttttgcga tatcaagtgc gcgcattgag
     7021 tcgctacgag ttgcattcgc catttctgtt cgatttggaa gagaaagtac tgcacgacaa
     7081 gcgccatttc tacgcctttg atgagattga atatcgcagg gagttactgc ttgctgaaga
     7141 tcaaaccatt tctgtgagcg attttggcgc cggcagccat gtgcataaat ccccggaacg
     7201 acgggtttcc gatattgcca aaacaagcct gatcacccca catttcggac aaatgctcta
     7261 ccggttagta cagtatttac aaccggttac cctccttgaa atgggcacat cgctaggaat
     7321 tagctccttg tatatggcta aagctgtccc tggtgcacaa ctcatcacgc ttgaaggcag
     7381 cagtgcgatt gcacaaatag cccgaaaaca acatatagca ctggatgcaa agaatattac
     7441 aataatggaa ggtgaattca atgctaccct accctcagca ctgaatgcct taaaaaaaat
     7501 agacctggca tttatcgacg gcaaccaccg atttgaacct acggtcgcct attttgagtc
     7561 tatttatcca tatttacacg aacactctat attggtgttt gacgacatac actggagtac
     7621 cggaatggat aaggcatggc atgtcatcaa atccgatgcg cgggtaactt gcagcatcga
     7681 tcttttctac aaaggaataa ttgcctttcg cccggaattc cgtcagccgc agcatattgt
     7741 tttgcgctat taagaccaat taacacagtt taaccgcact gtaaggcaat ctgccatcct
     7801 tcttaattac ctttatgcct tataataata cctatatgag aaaacgaatg atgataagca
     7861 tgtgtttact cctgctggca ggaatcatgc atgcgcaatc tccactaccc tccaacttct
     7921 tcctcaaaac cttgcccaat ggcttgcaaa tgctggtgat tgaagaccct accgttccac
     7981 ttgctacgat tgaaattgtt gtaaagaatg gttcttatac tgaggacagc acattcaacg
     8041 gcctttcgca catgtatgaa cacatgtttt tcaaggcgaa taaatcgctg ccaagtcagg
     8101 aagctttcct cgaaaaagtg caagaactgg gtgtagtatt taatggcacc accagtaatg
     8161 aacgcgtaaa ttatttcatc acattaagca atgcgaaatt aggcgaaggt cttgtattta
     8221 tgaataatgc cattcgctac ccattatttc tggagcagga aatgaaaaat gaaaatcctg
     8281 ttgttgacgg cgaatttcaa cgcaacgaat ctaaccctgt attcttcctg ttcgacgatt
     8341 tgaatcgtca tatgtggaaa tcaaattact atcgcaaaaa tgctatcggt ttacatgatg
     8401 tgattcttac ctgtacacct gaaaaaatgc gtatcatcca ggagaaatat tattatcctg
     8461 acaatagtct tattgccatt gccggcgatg taaaacatga tgatgtattt gcacaagtag
     8521 aaaaaatcta tggcgattgg aaaccgggtg gcttcgaccc attccagaaa tggcctatac
     8581 ctgaattcga acctttgaaa ggtgttgaga attttgttac tattaacgaa aatgctcaga
     8641 tcccgatgtt tatcacaggc tatcatggcc cggatactcg aaatgatgta aaagctactt
     8701 atgctgccga tgtttttagt tacttattaa atctgtcgga cagcaaactg cgccaggagc
     8761 tagtggaaac aggtcttgct tatgatgtca atgttggtta tcaaacatgt aaatacaccg
     8821 gacctatcaa tattttcctt gtaccaaatc ctgcaaaagt gagcgaagca tggaaaacat
     8881 tacaggaaaa tatcgggcaa tgggctagtc cgaattactt caccgatgaa caattggaaa
     8941 cagctaaaaa tcagctagcc attcaggatg cttatgacaa agaaagcatc agcaattatg
     9001 tacatactgt tacctattgg tgggcatctg catcgctgga ctacaataca aattacatta
     9061 aaaatgttca ggcagtgact cgtgaagata ttcagcgtta tgttgaaact tatatcatcg
     9121 acaagccgca tgtaaacgga ttattgttac atccggatat gcttcagatg gttgatgtga
     9181 aagcacttgg atttgaagta aaacaataat aacatttgaa cctcgaaaaa tacgttatga
     9241 aaaaaataca aataaccatc gttttattgg tgacactgct ttcagcaaat cgcttgacag
     9301 cacaggatac caaagaatat atggtggatg gcatcaaggt cattcataaa caggtaccta
     9361 aagatgtaat cagcgtgcag ctatttattg aaggtggcac tgccaattac acgaaagaca
     9421 aacaaggtat tgagaatctc acattgaatt tgatgatgga tggcggcaca accggcatgt
     9481 cgaaactcga attcaaatca gccgcagaaa aaatcggcac tacgtttaat gccggcagca
     9541 atatagacgc aggcacactt agcatgaatt gcattaaact attttgggac gattcatgga
     9601 aacttttttc cgatgctata ctccatcctg catttggcga agctgaatac aatttgctcc
     9661 gcgaacaatt aattgcaggt gcacaacaaa gcatggcaga tcctgacacg tatctagcct
     9721 tattatcccg ccaggtagca tttgaaaata aggattatgc taaagcaccg gaaggaactc
     9781 cggaaacgct aaccggcatc acactcgatg atgttaaaac ctattacaag aaaacaatag
     9841 gcaaaaaacg cgtattcctt gtagtagttg gaaatatttc tcaagaagac ctgactgcca
     9901 aaattcacaa tacgcttgca gcattacccg gcggaagccc ggcatcatct gaaagtcgca
     9961 cattgatcac gcaacctaaa acatatattg aagatcgcga catcgcaacc aattacattc
    10021 gtggtatcat gagtgctcca tttgcgaacg atgcggaagg tattgcgtat cgcgtagcga
    10081 tttcaattgt tggtgacaga tatttcaccg aattgcgcac aaaacgcagt ctcagctatg
    10141 ctccggcagc tttctatgca aacgcaggta tcaccaatcc ttatggcgtt atttatatca
    10201 ctaccatcga tcctaaacaa agtatgcagg tgatggtgga tgaattaaat aaaatcaaaa
    10261 aagaaggttt tactgaaaaa gaattgcgcg ataaacaaca ggaattctta actacctatt
    10321 acctcacact cgaaacaact gctaatcaaa gcaatgcatt gggtatggcg gaaatgagcg
    10381 gtggatggga aaatcttgat aaaataaccg ctgctgtaaa cagcctgaaa cttgaagaca
    10441 ttaatgctgt tttcgacaaa tacagcaatg ccattgtttg gacatatttg ggtaaaaaat
    10501 ctgctatttc tgaagctgat ttccctcaaa caacaccaaa ggaaaagaaa aacaaaccgt
    10561 attgattgcg gtattcatgg tataaaaaaa cctccgtcaa ttgcggaggt ttttttattg
    10621 ctgtaattta tcgtcactcg ttcaaaaaaa tctgtttgag ctcttttgct tcttccggtt
    10681 tcatacgtcc ggctaaaatc aaacgcaatt ctcttcttcg caacgccgaa acaaagcggc
    10741 gcttctcatc gtcattctca ggttctattt ccggaacgga aaccggtctg ccgtcatcat
    10801 ccaacgcaac aaaagtgaga taagcctcat tgcattttac tttttcatgc gtgcggaaat
    10861 tttctttgaa aacttctacg aatatctcca tactcgaatt aaatgctctc gtcatacgcg
    10921 ccgaaatagt tacgatgtcc cctattttga tcggacgacc gaaactcaca ttatctaccg
    10981 cagcggtaac tgctactcta ttggcatgct tgctggcaca tataccacag gcaatgtcca
    11041 tccatttcaa tagatttcct cccattaagt tgtgcatagg attggtatcg ttcggcataa
    11101 cgatctccgt aaaggtggtt gccgattctg aaacacgctt cgaagccata gtgtaaatat
    11161 tgccgcaaag gtaaccatta ttgccctatc gccttcagca tgccgatttg tggcattttg
    11221 agtatcattg tatgacttat caagctgccg taactgaact atttatttta tttatatgta
    11281 tatacataca aatgcgtaat ttagccctat ggagcgcagg aaattcatta aagcaggaac
    11341 atttagctta ttagccatgc aactaaagga attcagcaat acctttcaac aggaacaaga
    11401 tatcgtaatg cctgcaatgt ttgttgggca tggaaatccg atgaacgcca ttgaagacaa
    11461 tgaattcagt cgcggatggg aggcccaggg aaagttatta cctgttccca aagcaatttt
    11521 atgtatttca gcccactggc tcacgcgagg aacaagagta gctgccatgg aaaaaccgga
    11581 aacaattcat gatttcggcg gattcccgca aaccttattt gatgtgcaat accctgctcc
    11641 cggatcaccg gaatttgcaa aagaaaccag cactgtcatc accaaaacaa aagtagaact
    11701 tgattttgag tggggccttg atcatggatg ctggagtgta ttaaataaaa tgttcccaaa
    11761 tgccgatatc ccggtatatc aattgagtat cgactattat caaacacctg cttatcacta
    11821 tgcattggct gaagaattga aatcactccg caaaaaaggg gtattgataa tgggcagtgg
    11881 caatattgtg cataatcttg gcatggtgca atgggcagat actgcgtatg attgggcgat
    11941 tgaatttgac acgaagatca aagaattcat tgacgcgcga aatcaccaag gtgtaatcaa
    12001 ttataaagat ttgggtaaat cggcattact gtcagtgcca accaatgatc actttttgcc
    12061 attgatatat acactcggcg ttaccgatac caaagaagcg atctcatact tcaatgaaaa
    12121 aaataccatg ggcgccgttt ccatgcgctc atttgtggca ggattatcgt aatatccttt
    12181 tcaggtattc aacaatggta atttagataa aacctgcagc tatcattaat gccttttctt
    12241 tttaaataaa ggctgcttgt tagtattgcg ttctcgggaa gtaattttct cgcgcaaacc
    12301 cgggtttgat ttccttccgg caagtaattc ttcctgcact acaatattcg aagtgcccac
    12361 atttggattt ggattaacag tggtagcagc tattccttgc ccaccatgtg gaacaggttc
    12421 tgtatgttga acaaatccga cagacaatga agtgaaggcc gccggagaaa tgcgtgccga
    12481 ctgtccgaac acaggcatgg ccgcaataac aaaaaggata atgatgcaac gcttcataac
    12541 agtaaaacga aggtgtattg caaaattata tcagaaatgc atcggtgctt caattttgct
    12601 tgaatttgac aatgctgtga aatatgctta tcacagaatt agcggttaat agttgtgatg
    12661 tactgattac caacacatta gcctatttta atcgaaatga agccaagcgc attgtgtata
    12721 tttgccctca aaatgcaaaa tacatgaacc tgcacgaata tcagggtaaa gagctcgtaa
    12781 aacgctttgg cgttaagatt caggaaggca ttccggtaga tactccggag caagcagttg
    12841 aagccgcacg tcagttacag gcagctacag gtgctaatat ttgggtggta aaggcgcaaa
    12901 tccatgccgg cggtcgaggt aaggccggag gtgtgaaagt ggcaaaaagc cttgatgaag
    12961 tgtatgaaaa ggcttcaaaa atattaggtg ctacactcat taccccacaa acaggtgcaa
    13021 acgggaaaaa agtccataaa gtgcttgttg cagctgatgt gtactatccc ggcgcctctg
    13081 ataccaaaga attttacatg agcatcctgc tcgaccgcga gaaaagccgc aatgtaatta
    13141 tctattctac ggaaggtggt atggacatag aagaggtggc tgaacaccat ccggacaaac
    13201 tccataagga atgggtagac cctactgtag gtatccaggc atttcaggct agaaaaattg
    13261 ctttcaacct cggtttgagc ggtgctgcct tcaaggatat ggtgaaattc gtgacgagtc
    13321 tttataatgc atacgtgggt aacgactgtt cactgctcga gataaacccg gttttgaaaa
    13381 cctctgatga ccagatcatt gcggttgata cgaaatgtgt tgtagatggc aacgcgctgt
    13441 atcgccatgc agatattatg ggcatgcgcg atatcactga agaggatccg acggaagtag
    13501 aagccggtga agcaggtctc aacttcgtta aattagatgg caacgtggga tgtatggtga
    13561 atggagccgg attggcgatg gcaaccatgg atatcattaa agcatcaggc ggtaatcccg
    13621 ctaacttttt ggatgtgggt ggaacggcag atgcaaaacg cgttgaaacg gcatttaaga
    13681 tcatattgaa agatccgaat gtaaaagcaa ttcttgtaaa tattttcggt ggcattgtgc
    13741 gttgcgatcg cgttgcacaa ggtgttatcg atgcatataa aaacatcggc aatattccgg
    13801 tgccaattat cgtgcgctta caaggtacta atgccgaaga agctaagaaa atgatcgacg
    13861 acagtggact tcaggtgcgt tctgcaattc ttctgaaaga agctgcagca ctggttaagg
    13921 aagctgtgct ataatgtgaa ttctaacttt ttaaaataga aaaggatggc aaccccatcc
    13981 ttttttttat atccctaaaa taaaaatacc ccaccaattt ttcaaccgga ggggtaagtt
    14041 gttcaagtat gaaaagaaat tgcttgcgat ttgaactcag ctcaaaaata gatttgaatt
    14101 agtattttca agtattcgca ctttagccgg ggcacttgaa gcagcccccg cctggattct
    14161 tgcggcgggc aggcttaccg aaggttgtaa aggccattca agtaacgaag aataaagggc
    14221 gaagacatag aaaagacacc aaaggtatta ttgagatgtg ttctacataa tcattctaat
    14281 tacgcaatct gtaaacgccg aattaattca gtgtttgcac atgtttagct tcatcaagta
    14341 tgcgcatggc actttgtaaa atagcagtat cttccaatcg cactacaccg ccactgattc
    14401 ccttttcaaa atggacgtcg gcaatttttc ccggagcttc aaaatgttca gctccgaaat
    14461 aatttctgac ggcatccact tcaatgtgca gttcctcggc gatgcgctcc atactgccgt
    14521 gtggcaaggc atctttgatg tgtcgcaact cttcgaatgt gataaccata attgttcgat
    14581 ttaggtgaga aaattgtatt gcaatatacc acgatactag cccgcttttt cgatatttcc
    14641 aagaaaaaag tgaggttttt ttcgtttaat ttcatcattt ctggcattgc ttttgtggcg
    14701 aataaaaaaa cggggtttct atacatattt ttgtatgaat ttcatacgaa acaggtaatt
    14761 ttcatgcgtt attctatgca tcttctcatg tgaaccctaa accctccact accatgcggg
    14821 caaaactcat ctggcctggg cttcttgcac taggtttgta cgcttgtaac gccgacagga
    14881 atacaatagc catcaaaacc ggcgatttcc ctgaaacaat agaactcagg cagaatctgg
    14941 aaatacatct caaccagcat gtatttaacg attcgctatt cgacaaatgg gttgatgccg
    15001 atttgttgca gatagatcca cctgtagaag ggcgatttaa aatcgtttcc agcgatgctt
    15061 tctatttctc gccttctaaa ggatttgcag aaagtgaagc ctatactgca acacttggta
    15121 aggatctttt caattatacc gatctgaagt tagcactgaa atcagctccg gtaaactttc
    15181 atactacttt gctcgacctg acggcagtga atacatattg gtttctggaa cccacttcag
    15241 gggaagtaaa attgaatgcc ggactgcaat ttacagcctc agtggatccg caaacacttg
    15301 gcgatatgtt aacagtgaaa gttgatggta ctaataaaac tgcaagtatt acctccactc
    15361 aatcagctga taatatggca gttaccatca gtggcatatc tccaaaagct aaagatcaag
    15421 acctgaacat agacattcgc gccggaattg gatcaccggg ttcacgttac aaaacgccga
    15481 aacctttgca tatgaaagtt cctgttgctc cggtggaaga tcttgagatc agagatatta
    15541 ctgcaaacca tgacggcata gtgggaacta ttacggtaga atcaaatcaa cagttcagca
    15601 atactaatct tgaagaactg gtgaaaattg aaccctccat tccagttacc attgaaaaaa
    15661 ctttgaacgg actagttgtt accagcgaac aatttaatgc ctcacaaacc tatgtgatta
    15721 tactttcaga taaattgaga ggtgtagccg gaggaaaaat ggtagatacc tatcagcaat
    15781 ctgtcacctt cggagaactg gagccatcca ttgaattcac acaacaaaaa gcacgctatc
    15841 tcggcggtaa gggagaacgt aatatttcag taagaattgt caatgttcct aaagtgcagg
    15901 tcaatgttgt gaaaatttat gaaaacaata tccttgcatt cttgcgcgac ggcttctcat
    15961 gggattggta tgaatcatat gatgcagaag aagaatattg ggactaccac gattatcaaa
    16021 atgtaaatac atcgttgtat ggcaacgttg tttatacgga agaaatagaa acttcgaaac
    16081 tcgaaaagta taattccgcc agagtattgc atctcgattt tacagataaa ttggaacgct
    16141 tcgatggtat atatgtagta accgttagcg ctgctgaaaa agtatggcta accgatgctc
    16201 aaatcgtttc aatttcagac atcggcctca tagcaaaagc aacggaggat gaactggtag
    16261 tatttgccaa ttccattcag accgctgaac cgatgagtgg tattaaagta tcatttatca
    16321 gttccaacaa tcagaaactg tatacggcaa gcaccgataa aaacggagtt gcaaggttta
    16381 cgggattgaa acaaaacatg cccgatttca atgtaaatct aatcacggca aaaaatggaa
    16441 gcgactataa ttaccttccc tttagcgaaa ctgaagtaag cacttcgaga tttgatgtag
    16501 gtggtaaaag attaaatagt tctgatatag atgctttcct gtatcccgag cgcgatttgt
    16561 atcgtcccgg cgaaaccatg catattgcag gcattttgcg tacatacgac tggcaattgc
    16621 ttgaccgtat ccccgtgaag atcaaagtaa tctcgccgag cggaaaggat ttcaaaacac
    16681 tccgaaaaac tatcgatgat caaggtggat ttgaaacagg aatcgatatc ccgaaaaatg
    16741 ccgttacggg aacatatacc gttcaattgt tttcatcgaa tgatatttta ttggcatcgg
    16801 aatatatcag cattgaagaa tttatgcctg atagaatcaa ggtttccggt tcactcaata
    16861 aacagaatta ctatcccggt gaaagtgttc agtcgaccat agttgccaat aatctatacg
    16921 gcactccggc agctgagcgc aattacgaga gtgaatttaa tttgcaacag cactatttct
    16981 cttcaaaaaa atatcctgct tataattttc agatttccgg aggtaccacc tatttccctt
    17041 cgcaaaatgc cgaaggccag acagatgaaa atggtcaggc tgttcaatcc tacaccatag
    17101 atcaaaccta cagcaaggtt ggtcttctcg acggtcggat tttaaatact gtttttgacg
    17161 aatcgggccg acctgtttac caagtgcaga atttcaccgt catcacacaa agcaatctat
    17221 taggtatagg cgaatttgat tattacgtta aagtgaaatc acctttgcgc atcccaatga
    17281 ttgccgtcga taaagatgat aaaggattta agactcagga tgcccgagta gaagttatta
    17341 aatatgatta cgaaactgtc attgagaaac atcgcaatta ttattcatat cgctcgaaca
    17401 gaaaagagaa gatccttcaa tcctatgata agaaaatcag tggcgatcag gatggcattt
    17461 ggttcacccc gcaacaatcc ggagaatatc aaatacgctt atatttaaaa gatgctaaag
    17521 catacgttga aaagtcattc tatgcttatg gatggggttc aacacaaaac aattcgtttg
    17581 aagtaaatac agaaggtaac atcaatatca tttccgataa accaaagtat gatcttggcg
    17641 aagaagctga attcttattc actaccccat tcaatggaaa actattggtt acagttgaac
    17701 gcgaaaaggt aattgaatat cattacctgg acactgaaaa gaaagctgct tcattaaaaa
    17761 taaaaatgaa tcagtcgcac gtgccgaatg tatttgtaac agccacttta ttccgaccaa
    17821 acgacgaaag tggtatgcct ttgacagttg ctcatggcat catcaatgta gaagtaaaaa
    17881 atcctgcgaa ggcgataccg ttgacagtga cgcatgaaga tcattcacgc gcaaatacga
    17941 aacaaactat caatataaag acggcaccaa atgcgcaggt aactgttgct gttgttgatg
    18001 aaggcatttt gcaaatcaag aatttcacca caccggatcc ttacaactgg ttttacatgc
    18061 aacatgcttt aggtgtgttc agttatgatc tctatgctta tttgtatcct gaaatttatc
    18121 ttaagaagat gttgagcggt ggcgatgcca tgatgaatga aggcagattg aatcccatga
    18181 ccgcaaacag agtaaagctt gtgagttatt ggagtggtat cctaaaggca gatgcatccg
    18241 gaaaaatagc atacaccatt gatattcctc aattctccgg agatctgcgt gtaatggttg
    18301 cagcatatcg caacgatcag tttggtgcca gtgaatcgca tatgaaagtg agcgatccat
    18361 tggtaatgac tacttcccta ccccgcttcc tcactcccgg agatacgata gatgtgccaa
    18421 ttacacttgc caatacaaca gataaaaaag caagcggtaa agtgaccatg tctattgaag
    18481 gaccgatcaa agtggttggc agcagtacac aacaattcaa tgcaaatgcg aacaaagaag
    18541 ctcgaagtgt atttaaaatt gttgcctggg gagctacagg aaatgcgaag gtgaaagtcc
    18601 aatcttcagc attaggagaa acatttaccg acattactga aattccggta agaccgcctg
    18661 caggattgca gaaatacagt ggggaaggtt accttgacgg caataaatcg ataacggtta
    18721 atatgcaacc aaaccttatc agttcatctg ccaaggctcg cctgatgatc agttataatc
    18781 ctcttgttca atttgccgat gatcttgatt atctggtgca atatccgcat ggttgcgttg
    18841 agcaaatgac ttcaagtgcg ttcccgcaac tgtattatgc cgatatggtg aaatcattct
    18901 acagtaagga gcgtccggat cttgaccctg catataatgt tcaggaaact atacgcattt
    18961 tgcaaagcat gcaaatgtat aatggcgcgt tgacatactg gcccggaggt ggatatgaaa
    19021 gttggtgggg aagtatttat gccgcacatt tcttgcttga agcgaaaaaa gccggttacg
    19081 atgtagacca aaaaacacta gataaactat taggttattt acgcacgatg ttgaagcaga
    19141 aaaaaaccta tgtgtattat tatgataaag gtctcagtgt tgaaatggct gcacaggaag
    19201 tattttattc tatgtatgtt ctggcggtat cgggaaaggc tgaaatgtcg atgatgaatt
    19261 attacaaagc gaatctcagc atggtaccac aggatggtaa atatcttttg gccatgagtt
    19321 atatgctggc aggtgataaa cgcagtgcag ataaattgtt gccgggtggc tttaccgatg
    19381 cagatcccaa acaacaattc aatggcagct tcaacagtcc gattcgcgac agggcaattg
    19441 ccttgaactg tatgttggag gcagatccta ataatcagca aataggacgc ttgtcacaac
    19501 aactatcaga gatggtaaaa aatgctcgct atatgaatac gcaggaacgc gcatttacct
    19561 tccttgcatt cggaaaatta gcgaaacagt cgaaagaaca taatgcgaaa gttgtaatta
    19621 aaatcggagg caaagaagca ggcactttcg atggcacgga tttagttatt gaagatacca
    19681 aaatggcttc ttcaccggta acacttaatt cctcaggtag tggtcgcgtt tattatttct
    19741 ggtatgagga aggtgtttct gctgacggct ccgtaaaact tgaagataat tttttgaggg
    19801 ttcgcaagtc attttttaat cgatggggtc aaccaataga tcttaaaaat atccatcaga
    19861 atgatttggt tgttgttaag atcagcttgg taagtgctac cggcacctac gttgaaaata
    19921 tcgcgattac ggatatactt cctgcaggat ttgaaattga aaacgaccga ctccgtgcca
    19981 ctaattctat gccatggatt caaaatcagt cggaagcaca acatgaagat attcgcgatg
    20041 acaggatatt attgtacacg gatgctacgg ctaaggaaaa atatttctat tatacagtca
    20101 gagcagttac taccggaaca tttaaaatgg gaccgataat ggctgatgcc atgtataatg
    20161 aggaatatca ttcttacaat ggcggaggat gggtgaccat taaaaaataa gaaatgaaaa
    20221 aggggtgtta aagccccttt ttttatacct tataaattca atttgttttt attgtttgtg
    20281 gtgtgggttt tttcgtacta agacgggaag gggcgcaagg cgcgaagcat gtggtgtgga
    20341 ttggtggtgt cggttttttc gcgcggaggg cgcgcttgag ttattgatgc gatgaagttt
    20401 atcgactatt aaaaaatctg cgggaaaaaa ttttatgaac tttttttaca gtgcgttagt
    20461 taaccggcgt caggcatttt ttaatactta actcgaatac tactattaaa ctcctccgga
    20521 acagaaatta cagatccttt ttcgttgtag aatgtcattt tatactctcc tctatccaag
    20581 gcttcattta catctgtata tttatttaga aaatcctcta ctaccagcac ttctttccct
    20641 ttatcatccg ttaaaatcac ttttacattt gtattcgggc gtagaacatg ctgcaatttt
    20701 gccattttat tggcaccatt tactacccat gcaggtgagt ttggatctat agggtgctta
    20761 ccttttccgg atactactgt gtcttttact acggtaactt gtaatgcatc atccaccaca
    20821 acttttcctg cataattatt tgttccgtca gaaatgagat atttataatt tccggctcct
    20881 gctatatcta attccagcgt tggcccaacc tgagcggcat cgttattgaa aacaacggct
    20941 gaatctttta aaaccgtcaa cttatatgtc gctgcaattt tatcatatgg gatgttgaga
    21001 atggttttgc tattgataat aaacgccata gtttgcatat cggcagcagt ctcatcacct
    21061 attgtatcaa ccgtaacctc tccgggattt tccaaaaacc attgttgcga aacggggtca
    21121 gttcgccaga aatcaaccag atctttcgat actttttcaa ttcccttttc agtaggatgg
    21181 actccatcat ttgaaatcat atctctggtt aaggtatagc catctttgcg tggtttctca
    21241 ccttgcgtcc agaaatacgg tccccaaagt atcaacggag aaacggcatt tgcacctttg
    21301 aaagctaatt cctcgctgcc attgatctga ttttctatta attgcttaca cacccatcca
    21361 ttgatatagg ctttcggttc gcgatgttta tcctttgcat cggcaggcat aaaatcagtt
    21421 gtatgtcggc cggatacata aaacaatttt acattcggat aatgctcctt gcaaaaccta
    21481 accatgtagg tgaactcctt tgtcagttgg gcaatgcgta aatcgaaatt attctcgcga
    21541 ttgcggagat tatcctcttg aaaccaaata acctgaatct gattaagatc cattcctgct
    21601 tccctaacgc gctgatcaat aacactccaa tagtttgctg caggattcat gatatcactc
    21661 aaatcctgtc cgccaatccc acaatttaca aacgttagct ccttattaac tcctgctgct
    21721 tccggaatct gatgttccaa gcccttgcta aacatggcaa cggtacttgc accaagggca
    21781 ataaccccta tcctaccctt aggatccggt ttcccggact tgtcgagtgg ttgaaccgac
    21841 tgagccatag ccattgcatc ggcatagaat gcagcaggca tggaattgct gccattggga
    21901 tataatccac cctggcgacc cataaaagtg cctgtgccga gttcatcaat tggaggcaaa
    21961 tcattcacat cgccgctttg tgccttaaca aaaagggaac taaaaactga aaatatcgat
    22021 aatagcatca aacgcttcat ttgaggggtt tttattgaat tttaacaaat atcatacctt
    22081 tattgaaaag ttaggcgttt tttttgctgc aaggttatga tgtctatttc tattctcaca
    22141 cgttatatat tgttgttact gagtttcctc cccttttccg ccttttccca ggctatgttg
    22201 ggttatactg atatgcaagg tgattattat gtaagcgaca gtggaaggat agtgcatttg
    22261 gaacatcttc gcgtaaaatc ttcacaaccg gctgcaaaca gcatcgctta tatttcaaac
    22321 acgggaaatc tggtatatta cagtaatcat gaacaggtaa agctggatat caccaacccc
    22381 actttttata aaaataccga tcactatttg tattacagca caggaggaag tttttccgtg
    22441 tacaatggta aagaacgcaa gtacctggga tacatacaac aatatccgta tgccttcgcc
    22501 gatagcattg cagctgtgca cgactattcc ggatatttct atacctatgt aaatgacttg
    22561 ttcatcgaat tggaaaatca accggtttta aaggtaattg caggtgacaa tattctcgca
    22621 tacgtaaatc atattggtca gtttaaagct tattatcaag gtaataaggt tgatcttgat
    22681 gattacgctc ccatcactgc acaggctgcc ggaagtatca tcgcttacat cgataattac
    22741 caatacatga aagtatatta tcaaggtagc attcatgaat tatttaattt accggaaata
    22801 aattgtctta gcactacaag ttcaggggac cctcttcttg cttcatggtg taatggtccg
    22861 gtagtcacag acgttcaaag cgaactacct gttttccaag tgggtgatga catggttgcc
    22921 tatatcgatg atatggggcg atttaatgta ttctacaaag gcaatattgt tgcattggaa
    22981 agtcagccgc caaaacaata taccgtggtc gacagagttt tatattatac agacgaaaat
    23041 gactatctga aagtattttg caatggtgaa ttatcgattg tagaaaccta taccccatcg
    23101 cgtattgaag ctgatggtga agtattggct tatatggatc tggacaaacg attgaaggca
    23161 ttttatttaa atacatctgt tgatgtttcc gaaaatatag tattagattt cagtttgaat
    23221 aacaccttac tgatgtatag tgagatcccg aataaataca aattctattc cctcgaataa
    23281 aaatgggatt actccctaca tttagaaact tcttttcacg tgcacgtatt ttgcggataa
    23341 ttcatcatgc gcttgccaca gtgctgatag ttttcacctt gtttctgctg ttgaacctgg
    23401 catttccgat gcatgttaag attgattatt cgcaa
//
LOCUS       DAOQHY010000034        23259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_969_length_23259_cov_4.819988, whole genome shotgun sequence.
ACCESSION   DAOQHY010000034 DAOQHY010000000
VERSION     DAOQHY010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 23259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23259
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_969_length_23259_cov_4.819988"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PLL28_04490"
     CDS             complement(<1..471)
                     /locus_tag="PLL28_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68610.1"
                     /translation="MQKFYSSIAIAFICLSLQSLNAQGVCNPAGNVIIYSNYDGGNMT
                     INIDENIPDLHIGICSYESINVTFTGAYVDNITEVLYAGYDNDGTTSITGVDAGITDI
                     LLYPPVTLFDADGYPYMICAYECDSTYVPGGCNTVDQAVDYFMTTFGGSMRYSYF"
     gene            complement(579..3092)
                     /locus_tag="PLL28_04495"
     CDS             complement(579..3092)
                     /locus_tag="PLL28_04495"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNF68611.1"
                     /translation="MKSLKLFTLLLFSISRLHAQQPNIIFIITDDMNDYVGVLGGHPQ
                     VHTPNIDSLAKRGVTFINAFSSAPGCAPSRTSMLSGKDCGYTKVYSNEGYQSHFRDNF
                     SDVLGNSVVYTIPEILKDSGEYFTYGLGKIFHSPTENDYDKTGPTCEKEQSWNRMYAI
                     KSAPGYDELTESYKYGGIFDFGKTPDSFEEMMDDRIAVDSAVAFINSYAAHTVNTCDR
                     PFFLALGIHKPHTERFVPEHYFPQYYADDLLSDSFAIIYNNPPGATPFNGLVMPPQPD
                     PMFDDFYQLPEGGIAQNFAANNDTYALLNDYVDDLPYLPEIGEGLTTEEKVAILEETM
                     RANYVSAYIAAVQFADSMVGLVMEALYAHPELEENTIVVFASDHGYSLGEKRHWTKWT
                     LWETDIRMPLIISHPDYPQNVVSKAVVSLLDLFPTFCDMTGVPYPTMPDGSPYLDGHS
                     LVPLLMQPSMNFSNAALSSYRKSSGFGNCFPQYSVRTERFHYIRYQTNNANISGAGAC
                     DYTTPHYVEELYDIGSDRQTDPNEWHNIADDPNYAPVIDYMEEFIPGGTMYNQRPFTV
                     NIYQAPMPCLLEFDDVLQMRGLLLNDNGELIDSTLGNYTFTWTNNLTEEIHIGYEYNF
                     SLSSIPSDVFDDAKEIFIYLTVTETITGRTIAFKTKQNQLHGDAIPHLSYVVNIADHT
                     IAVDDITLTGNYQTINWDFGDSYKSSGLIPGPHTFNTAGYHTIDVTIEYGNKCYLSDT
                     RTFYIDSVIFKSTELFSDIKIFPNPASDVVIVSSDDINGKIQLRVFNAMGELVMSVSY
                     KDALESIQLQVDHLPAGVYLLQMERNGHVKEEMIEVCRN"
     gene            3396..4823
                     /locus_tag="PLL28_04500"
     CDS             3396..4823
                     /locus_tag="PLL28_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68612.1"
                     /translation="MKYFAHALKIVLLLLLVCCFNIPEAEAHVKWFTDGSYADRPLRL
                     GEILEGRFFYTLFFVTLGTVAIGVWLDARISKANWYMRVDNWLDSRKSYATIVLRVAA
                     AMVLILSWQSNAMIVPTIPIPANYEWLGWFQFLMAFMLLMPKTVPIGGLGIVVIWVIG
                     NITLHPFHMLDYLMFAGVGVYLVMASIKNGKYKDSGLVVLYVTVGFSLCWVALEKFVY
                     PNWSLFLIKEHPNLAMGLNFDFFLKSVGFVEFGLGFLLLVCLLQRPLAIIITVTFFIT
                     SSVFGKVEIIGHTLIHGALIVFLLEGPGRFYLQLRQRFSKMTMRIGFTIVNFTLLFVV
                     LTLGYTTLANNKYEKKIEFLSKKPEFQRGQIELAGYPKSELPGVWMEITKDAKDGYNI
                     HFNTTNFQFAPDKVNTEHVMGEGSINLFINGDKVARVYGEWYHIDIAAPGTYEVVATL
                     NSNELSDYCIRGTPISAREKITVSR"
     gene            4882..5610
                     /locus_tag="PLL28_04505"
     CDS             4882..5610
                     /locus_tag="PLL28_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNF68613.1"
                     /translation="MTEVDLFIPCFIDQMFPETGFNMVKVLEKAGCRVHYNPNQTCCG
                     QPAFNAGYWKEMEDVGKKFLADFANSDRYVVGPSGSCVGFVRNFYGELYANTPVHNYH
                     KNLKKNLFEFSEFLVDVLKVEDFGGKFPAKVTYHDACGALRECKIKEAPRKLMSHIAG
                     LELVEMQFPETCCGFGGTFAVKFEPISTGMADSKVNDAIASGAEYIVSTDYSCLMHIQ
                     AYIEKQNRNIKVLHIADLLAMSIA"
     gene            5655..6320
                     /locus_tag="PLL28_04510"
     CDS             5655..6320
                     /locus_tag="PLL28_04510"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNF68614.1"
                     /translation="MDEINQIASDIRRYQENGMRLFTSSSFQTHSTVLLHIISRIDPT
                     IPVFFLNTGFHFPETIQYKNEISQQFGLNTIDLRAATPKLMQVDDHGRFLFTSDPDYC
                     CYLNKIQPLEPMLDTYDVWINGVRADQNANRATFKKEAPTAHRAVRYHPMLGWSGKMI
                     YAYIREHQIPHHPLDAKGYTSIGCEPCTRKLSPEIMADKRQARWYGMSKTECGLHTEL
                     IKK"
     gene            6317..7243
                     /locus_tag="PLL28_04515"
     CDS             6317..7243
                     /locus_tag="PLL28_04515"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase"
                     /protein_id="HNF68615.1"
                     /translation="MKILITGGAGYIGYALAQRLSENDKVTEIILYDNLSRHNEIIFF
                     GNAIGNGKLKLIKADILDARKLRNAMKGVDVVYHLAAKVLTPFANQDPHFFEQINHWG
                     TSELVEVIEKSEVKHFIYLSSASVYDRVSEPATEDTIPTPNTYYGISKLRGEDHIKRI
                     PASTKTHIIRGGNVYGYSPSMRFDAVINNFLFQANFEHKLSIHGTGAQTRAFIHIDNA
                     SDALAQIPFSELPSGVYNMVTKNLAVNDIAEVICTLYPQCDRIFINQHLQLGSIRVST
                     DSPFWKYIQVEQRSLMEELADFKAKFAFSGWD"
     gene            7252..9156
                     /locus_tag="PLL28_04520"
     CDS             7252..9156
                     /locus_tag="PLL28_04520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HNF68616.1"
                     /translation="MHDIHAILKKHWGYDQFRPAQEQIIRAALDGRDTLALLPTGGGK
                     SVCFQVPALAMDGICIVVSPLIALMKDQVHHLKQREISAAAIFSGMSFREVDMTLDKC
                     VAGDIKFLYVSPERLLTPIFRERFKQMKLNLLAIDEAHCISQWGYDFRPPYLQIAALR
                     EFFPKVPVLALTASATPEVCEDIMLRLQFKEKNLIKGSFLRNNLSLIVRQQENKEDKL
                     LEILRSVPGTAVIYVRNRRKTKEVAQFLMRHQINASWYHAGLDQETRSNRQDQWINGK
                     LRVMVCTNAFGMGIDKSDVRVVVHLDIPESLEAYYQEAGRAGRDGKRSYAGLLFDIAD
                     VKVLDENYQKQYPEIAFIRQVYHQLGNYCKLALGAGENATFDFDMVAFCRQFQLPAVE
                     TRNALRILEQHNVIYVSDSLFKPSTIFIPVDKETLYRFQIENKQFEPVIKLILRTAPG
                     VFDDHTAIQETSMAWHLTITQPKLQEILQYLHDQEVLIYSPVKTKPQITFIHERIQAS
                     TLQLDYALLEQLKNAALNRLHAIKAYVNNHTDCRSVKLLQYFGEETPSCNVCDICVEK
                     NKLDLTEKEFRRIFDWLKEKLAGNALPPDALFQDKLPARKEKILETIRFLADNKQILH
                     TKDNILIWQE"
     gene            9153..10238
                     /locus_tag="PLL28_04525"
     CDS             9153..10238
                     /locus_tag="PLL28_04525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF68617.1"
                     /translation="MKHIVCTVTNDLTYDQRMDRICTSLTKAGYFVTLIGFVKTDSID
                     TLQKPYAQVRLRLMFKKGKLFYLEYNFRLFWYLLFRRFDIYNGIDLDTLLPVFVVAKL
                     KRKPIVHDAHEYFTELPEIVDRPVIQRAWKWLAKRILPHVKNNYTVGEAIATALQDSY
                     KNVYHVIRNVPVLNGNAPIEKGDYILYQGALNKGRGLEALMQAMQMVDAPLYIAGEGD
                     LSDELRNMAATLPHANKIVFLGYIRPDELKTFTAKAKIGINFVEPLGLSYYYSLSNKF
                     FDYIHAGIPQVTMDFPEYKRLNDLFNIALLIDEITPEKIAAALNTLLNDAVVYNKLAA
                     NTQAAKRELNWQREEEKLVKLYRQFEK"
     gene            10228..11352
                     /locus_tag="PLL28_04530"
     CDS             10228..11352
                     /locus_tag="PLL28_04530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF68618.1"
                     /translation="MKSKHLHIISFNVPYPPDYGGVIDVFNKIKALHAEGIKIHLHCF
                     EYGRNEAPELNAFCEHVYYYPRLPMWRGFFSRKPFLIASRKSNALRNRLLADEHPILF
                     EGLHACYLLDDPAFQNRIKLVRMHNNEPVYYKTLANRENRMFKKTYFLTEASRLSRFE
                     SVLSHAQHILCISPYEADHYEIRFPQTTYISAFHGHEAIHSKPGKGSFVLYHGNLSVN
                     ENDEAVKFILKEIWKELNVPLVIAGSAPSEILVRLIQEYPQVKLVQDPDHNTLEELIR
                     NAHIHLLPAMQQTGIKLKLLNAIFNGRFCIANRNMIEHTGLEQYCLCFNHADEAKAAI
                     NAYMQQEFTAEDVIFRQPVVDTFSDSASAKKIISYLPEHP"
     gene            complement(11327..12022)
                     /locus_tag="PLL28_04535"
     CDS             complement(11327..12022)
                     /locus_tag="PLL28_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNF68619.1"
                     /translation="METTSGNDIHFSGVSIFQGKTLVLSDVTFSIPHGEFVYLIGKTG
                     TGKSSLLKTIYGDLSLRSGSAEVAGFDLAKITWKTVPFLRRKLGIIFQDFQLLMDRSV
                     DDNLEFVLRATGTTDRSLIKLKSDEALKRVGLQSKGFKMPHELSGGEQQRVVIARAII
                     NDPVAIVADEPTGNLDPETSDDIMQLLRFLNKEYNTTILMATHNYNILEKFPARILKT
                     ANGKLITDVPVNS"
     gene            12107..13141
                     /gene="hemE"
                     /locus_tag="PLL28_04540"
     CDS             12107..13141
                     /gene="hemE"
                     /locus_tag="PLL28_04540"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HNF68620.1"
                     /translation="MALSNDLLLRVANRQSVERPPVWLMRQAGRILPEYRALREKVQG
                     FKNLVENPELVCEVTLQPVRALGVDAAILFSDILVVPEAMGLPYQMVESKGPWFENTI
                     DSPSDLSNLQIVDPEASLGFVLDGVKLIKGELNDSIPLIGFAGAPWTIFVYMVEGRGS
                     KTFSKAKAFLYRHPEASHKLLSMITEATIAYLKAQIKAGVNLIQLFDSWAGILSPEQY
                     ALFSMPYISRICDAIDEVPKTVFPKGAFFARQALSEINCNIIGLDWNMDIAETKQIVN
                     GRKALQGNLDPCVLYAEASVVKSETLKMLRAFGKDHIANLGHGVYPDTPLDNVKCFVD
                     TVKEFAYTGE"
     gene            13281..14546
                     /locus_tag="PLL28_04545"
     CDS             13281..14546
                     /locus_tag="PLL28_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68621.1"
                     /translation="MTFLGLTVFAEGQGYVGIGTTTPAEKLDVNGAIIVRGDATAATP
                     VAGTVRWNSTDGMHDGRTSAGEWRRLENNFSLLSGTYYNVSCGATLTTTVGAGTSTSA
                     TTYDTPFGTQYADNKRDYLYFASELTAAGYCAGTITALGFEIVSLGTSTINNFQIGMQ
                     STALTAISGTTFAGGYTIVYGGPGTTISLTAGVNYFTLTTPFTWDGTSNILIQVCFNN
                     GGVGYSGTNSSVKCNTTGVTVKTRYYYATLPSDGCTMATGTSTAVSRPNLYVTGKTTG
                     PIGTSGNYLYSALPWVVGTPAIPAPYVHHGDGTVTAQSLYDDNTLLSDHVFDYYFDGK
                     VADQDLGLHSDYKMASLEEMIAFMEEQRHLPTIQGRSAWKQNGKFSVGQLSTELWETS
                     ETQSLYILELNKRIEALKAKSDSLEPTKK"
     gene            14552..15958
                     /locus_tag="PLL28_04550"
     CDS             14552..15958
                     /locus_tag="PLL28_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68622.1"
                     /translation="MTTHLRAFIFALLLLPAITVFGQGEVGFGTDTPAEKADINGAMI
                     IRGAAVAATPILGTIRWNSTDGMHDGRETPGVWTRLENDEFEYNGDYTAMVCGTTSTT
                     SAGTATGTSTTASETPFATAYSDKRSQYLYYGTEIALGGLCAGYITQIGFQVSVLGAP
                     AALNGLTVSMKMTTTTSLSGLTWDTGLTTYYNGNYTLGPGPNYISLTSGAYPSGFYWD
                     GYSNIELELCYDNVSSSFNTTVEISSGLAYTATRTHYASPGVGCALTASVTEAKHPVL
                     YLTGTGVGPTTGVGDYLQFNLPIMIGSPVLPAPYVHHGPGSITAEAIYDENVIISDYV
                     FDNYFDGEMSETDKVKHADYTMRSIDEMTEYMRIHRHLPTIRGRNDWEENGKFSTGEL
                     ASELWVTYETHALYLKELHERTLVAEQLLMTSDEILKDAYLAEIFRLNQDNTLTESVK
                     SERIALLNQKIEELNHAK"
     gene            15969..17387
                     /locus_tag="PLL28_04555"
     CDS             15969..17387
                     /locus_tag="PLL28_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68623.1"
                     /translation="MKGIYLAAFLSVASLSAFSQGYTGIGTNTPQERLDVNGAIIVRS
                     DAVSPTPVPGTIQYNTTDGYHEGYTSAGTWIKLENENTIGYGDYITVVETCTSPATVG
                     SNEYTTPSTGYFNPNYEENPFSTWWMDDRTQLLYKASQLTAAGICAGNINAIGFNVVT
                     PGAYAMTSLTIKMKSTNITTLTAYETALSTVYSAGSYTPVVGANDFTLTSPFYWNGTD
                     NIVIEVCFNNSAWAAGCTVTGDMNVGYQSAVGYYADIAAAGLCSDNTTYFTDDLPQLR
                     VIGDVPTPTAGTGSYIQFSDAVVIGNPTLFYGGDYNGPGTLTVLGAYDDNTLISDYVF
                     DEYFDGKISVEDIKKHDNYKRYSISEMSEFVEKYRHLPTIEGRDAWEEKGGFSLGELL
                     TQLWVTAENQAIYISELNTDLKLVEQDIINNQDNIRASYNQALNRINSDALLSEEVKA
                     MAVQSINDRLRLLELLINAHKP"
     gene            17396..19078
                     /locus_tag="PLL28_04560"
     CDS             17396..19078
                     /locus_tag="PLL28_04560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68624.1"
                     /translation="MKRHILISSMCLLTAINMHGQLYVLGNGTNKEVYVDGTLVGEPA
                     LYINGYLNNNGGEVYNDAGEIELTGNWENYPGVAGSYESNGIERFTGAVNQIVKGTMN
                     GTTGNINQFYNLRVDKTAAGQFVSLQTNANVNPSGTVNFESNGIIRTDISSHGNNGSL
                     YAYELYVRNTAVASMIGYATSGTNKYIEGRLRRQVSGSGDYYFPIGIQSTFLDGEEPF
                     HINFASAPAANILSYVQPGTIDLLGTVMFCDVGTDPDPALGTTILDNPGSPPDGILDR
                     LETNCQYSVEWVATASLAGPYDYTITVEPGSVLQAECPFYSATWLGELKFTAKDGIPN
                     NAALASPAPFFTPGYMTCPNLFTIAGLSSFSVFRVHGITDGALVVLPIELLDFTGQTE
                     SLGNRLFWTTENETNNDYFVVESSADAASFKQIGIVDGAGTSTNRLSYNFLDEHPVAN
                     TTYYRLRTVDFAGAIGYSNTISLTRMSNEQLSVSPVPSMSSVTVVFAADASQEAIFTI
                     TGMDGKVMLNKKWNLKPGQNEQSVDITSWMPGTYVVQVDGVNAPLRTKLIKQ"
     gene            19149..20435
                     /locus_tag="PLL28_04565"
     CDS             19149..20435
                     /locus_tag="PLL28_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNF68625.1"
                     /translation="MVSEILLILLLVLINGLLAMSEIAMVSVKKVRLESAAKRGDIAA
                     KRALQLHNEPAKFLSTVQIGITLISILTGIFSGATIKLALAEDLAKIPVIEPYADTTA
                     LVIVVIAITALSLIVGELVPKRIGMRFPEPIAKAVAGPMLLLSAITKPFVWLLTKASD
                     GIIRILRLKASSEDQVTEEEIKAIIQEGTQTGAIDEIEQDIVERVFHLGDRRIASLMT
                     HRNDIIWLDVSEPIERVYEDIQSEVHSIYPVCDGEFDKVVGIVYIKDLFIARTKKEIT
                     SLRDLIKPVLFLPENNTAYDVLAKFKESKIHYALVVDEYGSSQGLVTINDILEALVGE
                     FDELNLPDYSIIEREDGSYLVDAALPFYDFLKYFDMEEEFEMLDELEYNTLAGFILHQ
                     LEHIPKEGERLDWKQYEFEIVDMDATRIDKVMFTLK"
     gene            20441..21289
                     /locus_tag="PLL28_04570"
     CDS             20441..21289
                     /locus_tag="PLL28_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68626.1"
                     /translation="MILIADSGSSKTDWALLHPVNGDVLPLTTKGLNPIVLPVDALIS
                     IMSANEHLLECATDVSAVYFYGAGCTGDVSIGNMHAALASIFKSAKIAVFSDMTGAVH
                     AVADNKPCIVSILGTGANSCLFNGKSISGNDFSLGYVLGDEGSGTWFGKRLIRDHWYC
                     WMPESIAAEFEKQYALTREEILKQTYRSPAPNAWLASFAPFIVQHRQHEYMQEVIFEG
                     LTDFFDAYINRFENSNALPLHFIGSLSCALEPEIRNVAEENALSCGKFIQSPISGLIQ
                     FYQSSH"
     gene            21298..22947
                     /gene="pgi"
                     /locus_tag="PLL28_04575"
     CDS             21298..22947
                     /gene="pgi"
                     /locus_tag="PLL28_04575"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNF68627.1"
                     /translation="MIPSESFRDSIIYKALTDDAERLKQMHLRQLFQDEERFASMSIF
                     WDELLFDFSKNRIDSKAWKNLHRLAEEMQLTEAFEGMLRGDKINVTEGRPVLHTALRN
                     FSDSEVLVNGKDVMPDVREVLQKMKIFSDQIISGEKRGYTGKQFTDVVNIGIGGSDLG
                     PVMVCEALKHYHVPHLKVHFVSNVDGSHITETIKNLNPETTLFLIASKTFTTQETMAN
                     AHTAKQWFLHTAQHEQHVAAHFVALSTAEKEVTAFGIDRENMFVFWDWVGGRYSLWSA
                     IGLSICLAIGYELFEDLLYGAHQVDNLTKSVEPEHNIPLTMALLGVWYNEWFDAHTHA
                     VIPYDQYLHRFTAFLQQADMESNGKSVQRNGKRVDYATGPVVWGEPGTNGQHAFFQLM
                     HQGTQLIPADFIVAATPLHNIMEQHHMLLSNCLAQTEALMNGKTEEEVRTELRQKGMD
                     NETINKHLPYRVFEGNRPTNTIVIDQLTPYNLGRLIAIYEHKIFYQGILWNVFSFDQW
                     GVELGKKLAAEILPEWTSKETLRHHDSSTGGLIQYIRNLLG"
BASE COUNT         6836 a         4727 c         5015 g         6681 t
ORIGIN      
        1 aaaatatgaa taccgcatgc tgccgccaaa ggtggtcata aaatagtcta cagcttgatc
       61 aacagtatta caacctccgg gaacataggt tgagtcgcat tcataagcgc aaatcatgta
      121 tggataacca tcggcatcaa ataaagttac cggtggatac aataaaatgt cggtaattcc
      181 tgcatcaaca cccgtaatgc tcgttgttcc atcattgtcg taaccggcat acagcacttc
      241 ggtaatgtta tcgacatagg cacctgtaaa tgttacattg atagattcat atgaacaaat
      301 gccaatgtgc agatccggaa tattctcatc aatattgatg gtcatattcc caccatcata
      361 gttagaataa atgatcacat taccggcggg attgcaaacg ccctgagcat taagcgattg
      421 aagtgacagg caaatgaatg cgattgcgat tgaggagtag aacttttgca tgcgtttttt
      481 ttataaaaat aacaaaaacg ccataaccat cctaattatc atttcctttt aacattacat
      541 cccggtaaga caactctccc gggaatttat taatgtatct aattcctaca tacctctatc
      601 atttcttcct tcacatggcc attgcgttcc atttgtaaaa gataaacccc tgcaggtaaa
      661 tgatctacct gtaattgaat ggattctaat gcatctttat aagatacaga cattacaagt
      721 tcgcccatag cattgaatac tcgaagttgg attttgccat taatatcatc tgagctcacg
      781 attaccacat cagatgccgg atttgggaat attttgatat cggaaaacaa ctctgtcgat
      841 ttaaatatca cactgtcaat ataaaatgtc ctggtatcag agagatagca tttgttgcca
      901 tattcaatag tgacatcaat tgtatggtag ccggctgtat tgaaagtatg gggtccggga
      961 atcaaacctg atgatttata gctgtcgccg aaatcccaat taatggtttg atagtttcct
     1021 gtcaatgtga tatcatcaac cgcaattgta tgatctgcta tattaaccac ataacttaaa
     1081 tgcggaatgg catcgccatg taattgattt tgttttgttt taaatgcaat ggttctaccg
     1141 gttatagttt ctgtaaccgt aagataaata aatatctcct tggcatcatc aaatacatcg
     1201 gaaggaatac ttgacagact gaaattatac tcatagccga tatgaatttc ttcagtaaga
     1261 ttatttgtcc acgtaaatgt gtaattccct aatgtagaat caattaattc tccgttatca
     1321 ttgagtaata ggccccgcat ttgtagaaca tcatcaaatt caagcaagca aggcattgga
     1381 gcctgataaa tattcaccgt aaatggtcgt tggttgtaca tggtaccgcc gggtatgaac
     1441 tcttccatat aatcaataac cggcgcgtaa tttggatcgt cggcgatatt atgccattca
     1501 ttaggatccg tttgacggtc gctgccgata tcatataatt cttctacata atgaggtgta
     1561 gtgtaatcac aagctccggc accactgatg tttgcgttat tagtttgata tctgatataa
     1621 tgaaatcgct ctgttcgaac ggaatattgc gggaaacaat ttccaaaacc agaacttttg
     1681 cgataagaac tcaatgcggc attagaaaag ttcattgatg gttgcatcag caacggcacc
     1741 aggctatgtc catcaagata cggtgagcca tcaggcatgg taggataggg tacacctgtc
     1801 atatcgcaga aagtaggaaa caaatctaat aacgaaacca cggcttttga cacaacattt
     1861 tgcgggtaat cgggatgcga aataattaat ggcatacgta tatccgtttc ccataaagtc
     1921 cattttgtcc agtgacgttt ttcacccaat gaatatccat gatcacttgc aaaaacaaca
     1981 atggtgtttt cttccaattc gggatgcgcg tataaggcct ccatcaccaa accaaccatg
     2041 gaatctgcaa attgtactgc tgctatataa gcagatacat aatttgcacg catggtttct
     2101 tctaaaatag caactttttc ttcggtggtt aatccttcgc caatttcagg taaataggga
     2161 aggtcatcta cataatcatt tagcaatgca taagtatcat tattagctgc aaagttttgt
     2221 gcgatgcctc cttcaggcaa ttgatagaaa tcgtcaaaca tgggatccgg ttgcggcggc
     2281 ataaccaagc cattaaaagg agtagctccc ggaggattat tataaataat agcaaatgaa
     2341 tcactcaata aatcatcagc ataatattgc gggaaataat gctcaggtac aaatctttca
     2401 gtatgcggct tatgaatgcc taacgcaaga aaaaatggtc tgtcgcaagt atttacagta
     2461 tgagccgcat aactattgat aaatgccact gctgaatcaa cagcaattct gtcatccatc
     2521 atttcttcga atgaatcagg tgttttccca aaatcgaaaa taccgccata cttgtaactt
     2581 tcagttagtt catcataacc cggcgccgac tttatggcat acatgcggtt ccacgattgt
     2641 tccttttcgc atgttggacc tgttttgtcg taatcgtttt cagttggtga atgaaatatt
     2701 tttcccaaac cataagtgaa atattcacca ctgtctttta gaatttccgg aatggtatat
     2761 accaccgaat tgcccagcac atcggagaaa ttatctctga aatgactttg atatccttca
     2821 tttgaatata ccttagtata tccgcaatct tttccgctta acatacttgt tcgcgatggt
     2881 gcacaaccgg gagcactgct gaatgcatta ataaaggtta caccgcgttt agcaagactg
     2941 tcaatattag gcgtgtgaac ctgaggatgt ccaccaagaa ccccaacata atcattcata
     3001 tcgtcggtta tgatgaaaat gatattgggt tgttgtgcat gcaatcttga aatgctaaat
     3061 aatagcaggg taaaaagttt cagtgatttc atcatcggga attgtaaatc aaaactgcag
     3121 ccaactgccg gttcgtgcaa ccgattctta tcaagaccac gtcattttca tgtgaaacaa
     3181 ccttataact gtgtgaagtg gttttgttac ccaattaatt ggggggaatt gacttggaat
     3241 tcccatgcag gtaaaaacca tagattgtcc tcaccaagcg tcattacgcc gaaaattgtg
     3301 aagaacatat atggctgttt acatttcttt tacactgcct tggcattagg cgcaatagca
     3361 gtgtttagct ttgccaatac ccttgtcctg ttgttatgaa gtactttgca cacgcattaa
     3421 agatcgtgct gctgctattg ctggtatgtt gtttcaacat tcctgaagct gaagcgcacg
     3481 tgaagtggtt taccgatggt agttacgccg ataggccact cagactcggc gaaattcttg
     3541 aaggccggtt cttctatacc ttgttttttg tcaccctggg gactgttgca atcggtgtat
     3601 ggcttgatgc acgtatttcc aaggctaatt ggtatatgcg tgtcgacaat tggctcgact
     3661 cgcgcaaatc ctacgccact attgtactgc gtgttgctgc agctatggtg ttgatccttt
     3721 catggcaaag taatgctatg atagtgccaa ctattccaat tcccgcaaat tacgagtggc
     3781 tcggctggtt tcagtttctc atggctttca tgttgctgat gccaaagact gtgccgattg
     3841 gagggcttgg tatcgtggtg atctgggtga tcggtaatat cacgctgcat cccttccaca
     3901 tgctcgatta cctcatgttt gcaggtgttg gtgtgtacct ggtgatggca tccattaaaa
     3961 acgggaagta taaagactca ggactggtag tgttgtatgt aacggttggc ttctctctct
     4021 gttgggtggc actcgaaaaa ttcgtgtatc ctaattggag ccttttcctt atcaaagaac
     4081 atccgaatct ggcaatgggg ttgaatttcg acttcttcct caaatccgtt ggattcgtag
     4141 aattcgggct agggtttttg ttgttggttt gcttactcca acgtccattg gccattatca
     4201 tcactgtaac cttctttatt acatcctctg tattcgggaa ggtcgaaatt attgggcata
     4261 cactcattca cggcgctttg atcgtgttcc tgctcgaagg tccggggcgc ttttatttgc
     4321 aattgcgtca gcgttttagc aaaatgacca tgcgcattgg ttttaccatc gtaaacttta
     4381 ccttgctgtt tgttgtgctc acattgggtt ataccactct tgcaaataat aagtatgaaa
     4441 agaagattga atttctaagc aaaaagcctg aatttcagcg tggacagata gaacttgccg
     4501 gttatccaaa atcagaactg ccgggagttt ggatggaaat taccaaggat gcaaaagatg
     4561 gatataatat tcattttaat acaactaatt ttcaatttgc tccggacaag gtaaataccg
     4621 agcatgttat gggtgagggt agcatcaacc tcttcatcaa cggcgataag gttgccagag
     4681 tttatggaga gtggtatcat atcgacattg ccgccccggg aacttacgaa gtcgtggcta
     4741 cgttgaactc caacgaactg agcgattact gtatacgagg aactccaatt tcagcacgcg
     4801 aaaaaattac agtcagcaga taacagccat tgtgcaaaag tcctgcttac gcctttctat
     4861 cggttcatta catttgctgc catgacagaa gtggatttat tcataccttg ctttatcgat
     4921 cagatgttcc ctgaaacagg gtttaacatg gtcaaagtgc ttgaaaaagc aggctgccgg
     4981 gtgcattata atccgaatca gacatgttgc gggcaacccg ccttcaacgc cggctattgg
     5041 aaggaaatgg aggatgtagg gaagaaattt cttgcggatt tcgcaaattc tgatcgttat
     5101 gttgtaggtc catcaggctc ctgcgttggc tttgtgcgca atttttatgg cgaactttat
     5161 gccaacacgc cggtgcacaa ttatcacaaa aaccttaaga aaaacctctt cgagttctcc
     5221 gaatttttgg tcgacgtgct caaagtggaa gattttggag gcaagtttcc tgcaaaagtg
     5281 acttatcacg atgcttgtgg cgccctccgt gaatgtaaga taaaggaagc tcctcgtaaa
     5341 ctcatgagtc atatcgcagg actggaattg gttgaaatgc aattccctga aacgtgctgc
     5401 ggttttggag gcactttcgc cgttaaattt gaacccatca gcacaggtat ggccgacagt
     5461 aaggtaaatg acgctatcgc ttccggtgcc gaatatattg tgtctaccga ctactcctgc
     5521 ctcatgcaca ttcaggcata tatagaaaag caaaatcgca atatcaaagt gttgcatatt
     5581 gcagacctgc tggcaatgag cattgcctga cgacaaaata gcggctaaaa aatggccggt
     5641 atgatttgag cattatggac gaaataaatc aaatagcatc agacatcagg cgatatcagg
     5701 aaaatggcat gcgtttattt acaagctcct ctttccagac acattctact gtattgctgc
     5761 atatcatttc ccgcattgat cctactatcc ccgttttctt tttaaatacc ggatttcatt
     5821 ttcctgagac tattcagtac aaaaatgaaa tcagtcaaca gttcggatta aacaccatcg
     5881 accttcgcgc tgcaacgcct aaattaatgc aggttgatga ccacggtaga tttctgttta
     5941 cttcagaccc tgattattgc tgctatctga ataaaataca accactcgaa cccatgctcg
     6001 atacctatga tgtatggata aatggtgttc gtgccgatca aaatgcaaac agagcaacat
     6061 ttaaaaaaga agctcctacc gcacacagag ctgtcagata tcatcctatg ttgggatggt
     6121 cgggtaaaat gatatatgcc tatatcaggg aacatcaaat tccacatcat ccgcttgatg
     6181 ccaaaggata taccagcata ggttgtgagc catgcacaag aaaattaagt cccgaaatta
     6241 tggccgataa gcggcaagcc aggtggtatg gtatgtcgaa aacagaatgt gggttacaca
     6301 ctgaattaat aaagaaatga aaatcctgat caccggcgga gccggatata ttggttacgc
     6361 attggcacaa cgattatctg aaaatgataa ggtaacggaa attatattat atgataatct
     6421 gagtcgacac aatgaaatca ttttctttgg aaatgcaatc ggcaatggca aattaaaatt
     6481 gatcaaagct gacatccttg atgcgcgtaa attgcgcaat gcaatgaagg gtgttgatgt
     6541 tgtatatcac cttgctgcaa aagtacttac accttttgca aatcaggacc cgcatttttt
     6601 tgagcagatc aatcactggg ggacgagtga actcgtagaa gttattgaaa aatctgaggt
     6661 taagcatttt atttatttgt ccagtgcttc tgtttacgac cgtgtttctg aaccggctac
     6721 agaagatacc atccccacac ctaatactta ctacggaata agtaaacttc ggggagaaga
     6781 tcatattaaa cgcatccctg cctcaacgaa aacacatatt atcagaggtg gcaacgtata
     6841 cggttattcg ccctccatgc gttttgatgc cgtgatcaat aattttttgt ttcaggcaaa
     6901 tttcgagcat aaactttcca tacacggaac cggagcacaa acacgcgcat ttattcatat
     6961 cgataatgca tcagatgcgc tggctcaaat accgttcagt gaattgcctt ccggcgttta
     7021 taatatggtc acgaaaaatc ttgcggtgaa tgatattgct gaggtaattt gcacgcttta
     7081 tccgcaatgc gacaggatat tcattaatca acatttacaa ctcggaagta ttcgcgtaag
     7141 caccgattcg ccattttgga agtacattca ggttgaacaa cgaagcctga tggaggaact
     7201 ggcagatttt aaagctaaat ttgcctttag cggttgggac tgatttcatc tatgcacgat
     7261 attcatgcta tccttaaaaa acactggggt tacgatcagt ttcgcccggc tcaggagcaa
     7321 attatccgtg ctgctctcga tggtcgcgat acgcttgcat tattgccgac aggtggcgga
     7381 aaatcagtct gctttcaagt gcctgcattg gcaatggacg ggatatgtat tgtggtttct
     7441 ccattaattg cactgatgaa agaccaggtg catcacctaa aacaaagaga gatatctgca
     7501 gctgcaattt tttcaggaat gtcgttccgc gaagtggata tgaccttaga taaatgtgtt
     7561 gcaggcgata ttaaatttct ttacgtttct ccggaacgat tgcttacacc aatttttcgt
     7621 gaacgattta agcagatgaa attaaatctg ctcgccattg atgaggcaca ttgtatctcg
     7681 cagtggggat acgatttccg tccgccgtat ttgcaaatag cagcattacg ggaatttttc
     7741 cccaaagtgc cggtactcgc gcttaccgct tcggcaactc ctgaagtatg tgaggatatc
     7801 atgttgcgtt tgcaattcaa agaaaagaac ctcatcaaag gaagtttctt gcgtaacaat
     7861 ttaagcttga ttgtgcgcca acaggaaaac aaagaggata aactactcga gattttgcgt
     7921 tctgttccgg gcacggcagt gatttatgtc cgcaatcgtc gcaaaacaaa agaagtagcg
     7981 caatttctca tgcgccatca gattaatgct tcatggtatc atgcaggtct tgaccaggaa
     8041 acacgcagca atcggcaaga tcagtggata aatggaaaat tgcgcgtaat ggtttgcaca
     8101 aatgcctttg gtatgggcat agataagtct gatgtaaggg tagtggtgca cctcgatatt
     8161 cctgaatcat tagaggccta ttatcaggaa gcagggcgtg ccggtcgcga tggaaaacgt
     8221 tcttacgcag gattgttatt cgatattgca gatgtgaaag tgctggatga aaattatcaa
     8281 aagcaatatc cggaaatagc cttcatccga caggtgtacc atcagttggg aaattattgt
     8341 aaactcgcac ttggcgccgg agaaaatgca actttcgatt ttgatatggt cgcattttgc
     8401 cggcaatttc aacttcctgc cgttgaaaca agaaatgcat tgcgcatact cgaacagcac
     8461 aatgtgattt atgtaagcga ttctctattc aagccatcaa ccatatttat tccggtagat
     8521 aaggaaacat tatatcgctt tcaaatagaa aataaacaat ttgagccggt aattaaatta
     8581 atcctgcgca cagcacccgg cgtgtttgat gatcataccg ctattcagga aacatctatg
     8641 gcctggcatc taacgattac tcaaccgaag ctgcaggaaa tattacaata tttacatgat
     8701 caggaggttt tgatttatag tccggtaaaa acaaaaccac agataacatt tatccatgaa
     8761 cgcattcagg cttccacgct tcaattggat tacgccttgc tggagcaatt aaaaaatgct
     8821 gcgttaaata ggcttcatgc tattaaggca tatgtaaata atcataccga ttgtcgcagc
     8881 gtcaaactat tacaatattt tggtgaagaa acgccttctt gcaatgtttg cgatatttgt
     8941 gtagaaaaaa acaagctcga tctcacagaa aaagagttcc gtcgtatttt tgattggttg
     9001 aaagagaaac tggcaggtaa tgcgttgccg ccggatgcac tttttcagga taaattacct
     9061 gcaagaaaag agaaaatttt ggaaacgata cggttcctcg ctgataataa gcaaatactg
     9121 cacacaaaag acaatatcct catttggcaa gaatgaaaca tatcgtttgc acagttacca
     9181 acgatctgac ctacgatcag cggatggaca ggatatgtac ttcgctcacc aaagccggat
     9241 attttgtgac attgattggg tttgtgaaaa cagattctat tgacaccttg caaaaacctt
     9301 atgcgcaggt gcgattgcga ctaatgttta aaaaaggtaa actattttac ctggaataca
     9361 atttccgatt gttctggtat ctgctttttc gtagattcga catctataat ggtattgatc
     9421 tcgataccct gttacccgtt tttgttgttg caaaattgaa gcgtaaacct attgtgcatg
     9481 atgcgcatga atattttacc gaattacctg aaattgtaga tcgtcctgtt attcagcgcg
     9541 cttggaagtg gctggcgaaa agaattttac cgcatgtgaa aaataattac actgtgggtg
     9601 aggctattgc aacagcactg caagattcat acaaaaacgt atatcatgta atcagaaatg
     9661 tgccggtatt gaatggaaac gcgcctatag aaaaaggtga ttatatttta tatcagggtg
     9721 ctctcaacaa gggtagggga ctcgaggctt taatgcaagc tatgcaaatg gttgatgcgc
     9781 ctttatacat tgccggcgaa ggcgatttga gcgatgaatt gcgcaatatg gcagcaacat
     9841 tgcctcacgc caataaaatt gtatttctcg gctatatccg acctgatgaa ttgaaaacct
     9901 ttaccgctaa agcaaagatc ggtatcaact ttgttgagcc actgggattg agttattatt
     9961 attctttgtc gaataaattc ttcgattaca tccacgcagg tatccctcag gtaaccatgg
    10021 atttccctga atacaaacga ttgaatgact tatttaacat tgcactgcta attgatgaga
    10081 ttacccctga gaaaatagcg gcggctttga atacattact caatgatgca gttgtttata
    10141 ataaactggc agccaacacc caagcagcaa aacgcgagtt gaactggcag agggaagaag
    10201 aaaaattggt aaaattgtat cgtcaatttg aaaagtaaac atctacatat catttctttc
    10261 aatgtgccat atccaccgga ttatggtggt gtgatagatg tgtttaataa aatcaaagca
    10321 ttgcatgcag aaggaattaa aattcatctc cattgctttg aatatggtcg aaatgaagcg
    10381 cctgaattaa atgctttttg cgaacatgtt tattattacc cgcgtttgcc catgtggcga
    10441 ggattctttt cgcgcaagcc atttttaatc gcgtcacgca aatctaatgc actgagaaac
    10501 aggttactcg ccgatgagca tccaattctg tttgaaggat tgcatgcgtg ctatctgctg
    10561 gatgatcctg cttttcaaaa tagaataaaa cttgttcgta tgcacaacaa cgagccggtt
    10621 tattataaga cactcgcgaa cagagaaaac cgtatgttca aaaaaacata ttttctcacg
    10681 gaggcttcaa ggttgtccag atttgaatcc gtgcttagcc acgcgcaaca tatactttgt
    10741 atttcgcctt acgaagctga tcattatgaa attaggtttc cgcaaactac ttatatttct
    10801 gctttccatg gacatgaagc aatacattca aaacccggaa aaggaagctt tgtattgtat
    10861 catggtaacc tcagcgtaaa tgagaacgac gaagccgtga aatttatatt gaaagaaata
    10921 tggaaggaat tgaatgtgcc acttgtaatt gcaggctcag ctccttctga aattcttgtc
    10981 cggttaatac aggaatatcc gcaggtgaaa ttagtgcaag atcccgatca taataccttg
    11041 gaagaattga tccgaaacgc acatatccat ttgttgccgg caatgcaaca gaccggtatt
    11101 aaattgaagt tgttgaatgc gatatttaat ggaagattct gcatcgccaa cagaaatatg
    11161 atcgagcata ctggattgga acaatattgc ctttgtttta atcatgccga tgaagcaaaa
    11221 gcagcaataa atgcctatat gcagcaagaa tttactgctg aagatgttat tttcagacag
    11281 ccggtagtcg acaccttcag cgatagcgcc tctgctaaaa aaattatcag ctatttaccg
    11341 gaacatccgt aatcaatttt ccgttagccg tttttaaaat gcgagcaggg aatttttcca
    11401 gtatattgta attgtgtgtt gccatcagaa tcgtcgtgtt atattcctta ttcaggaaac
    11461 gcaataattg cataatatca tcacttgttt caggatccag atttcctgta ggttcatctg
    11521 ctacaatagc aaccggatcg ttaatgatgg ctctggctat taccacgcgt tgttgttcac
    11581 cacccgataa ttcatgcggc attttaaatc ccttcgactg caaccctacg cgctttaatg
    11641 cttcatcgga ttttaattta ataagcgatc tgtcagttgt gcctgttgcg cgcaatacaa
    11701 attccagatt atcatctact gacctatcca tcagcaattg aaaatcctga aaaatgattc
    11761 ctagtttcct gcgcaaaaat ggtacagtct tccatgtgat tttagcaaga tcaaaaccgg
    11821 caacttcggc actgccgcta cgcaaagaaa gatcgccgta aattgttttg agcagactgg
    11881 atttcccggt accggtttta cctatcaagt acacaaattc tccatgcggt atgctgaagg
    11941 tgacatcact caacaccaaa gttttaccct gaaaaatgct tacaccggaa aagtgaatat
    12001 cgttaccgct tgttgtttcc atgtggcgaa gttacctgct gtcatgccaa ggtaaatatc
    12061 cacttatgca caatagattt cggcctcgct cttgtaactt tgcaccatgg cattatcgaa
    12121 tgatttgctg ctgcgtgtcg ccaatcgtca atcagtagaa cgcccgccgg tatggttaat
    12181 gcggcaggca ggaagaatac tccccgaata ccgtgcactg agagaaaagg tacaagggtt
    12241 taaaaacctg gtagaaaacc ccgaattagt ttgcgaagta acccttcaac cggttcgcgc
    12301 ccttggtgta gatgccgcca tcctgttttc cgatattctt gtggtgcctg aggccatggg
    12361 acttccatac caaatggtgg aatccaaagg cccatggttt gaaaatacaa ttgattcacc
    12421 ctcagacctg tcaaaccttc aaatcgttga tcctgaggct tctttaggct tcgttttgga
    12481 tggagttaaa ttgatcaaag gggaattgaa cgacagcata ccccttattg gcttcgcagg
    12541 agccccgtgg acgatctttg tctacatggt agaggggaga ggctctaaaa cctttagtaa
    12601 ggccaaggct tttctttacc ggcatcctga ggcctcgcac aagcttctca gcatgatcac
    12661 cgaggctacc attgcatatt tgaaagctca gatcaaggcc ggcgtgaacc tgatacaact
    12721 cttcgactcc tgggcaggaa ttcttagtcc tgagcaatat gctctgttca gtatgccata
    12781 tatcagcaga atttgcgacg ccattgatga agtgccgaaa accgtattcc ccaaaggagc
    12841 tttcttcgcg cgtcaggcat tgtcggaaat caattgcaat ataatcggac ttgattggaa
    12901 tatggatatt gcagaaacaa agcaaattgt caatggcaga aaggctttgc aaggcaatct
    12961 ggacccctgt gtgctctatg ccgaggcatc ggtagtaaaa agtgagaccc tcaaaatgct
    13021 gcgtgcattc gggaaagatc atatcgccaa cttaggacat ggagtttatc ctgatacacc
    13081 attagacaat gtaaaatgct ttgtcgatac cgttaaagaa tttgcataca ccggcgaata
    13141 aattctatta ttttcatgta tacacatggt tgtgcaaaaa aaatgtaaat acgggcttgc
    13201 atagtcctat gcagaatggt atgtttgttt aggagaacat acatcaaatg aggacttttt
    13261 acccgaaatt cctttttatt gtgacatttc tgggcctgac ggttttcgcc gaagggcagg
    13321 ggtacgtagg cattggaact actactccgg ctgaaaaact agatgtgaac ggtgccataa
    13381 tagttagagg tgatgcgacc gccgctaccc ccgttgccgg cacagtcaga tggaacagca
    13441 ccgacggaat gcacgatgga aggacatcag ccggcgaatg gcgccgacta gaaaataact
    13501 tttccttact ttcaggcacc tattataacg tgagttgcgg agctacgctt acgactaccg
    13561 ttggtgccgg aaccagcaca agtgccacca cgtacgacac gccttttgga acgcaatacg
    13621 ccgataacaa gcgcgactac ctgtattttg cttcggaatt gactgctgca ggttactgcg
    13681 caggaaccat cactgctttg ggctttgaaa ttgtatctct gggaacaagc accatcaaca
    13741 acttccagat aggtatgcaa tctacagcat taactgctat ttcgggtact acatttgcag
    13801 gtggttatac aattgtttac ggaggacccg gaaccacaat ttcccttaca gccggtgtaa
    13861 actattttac ccttactacg ccctttactt gggatggaac aagcaatatt ttgattcagg
    13921 tttgctttaa taatggaggt gtcggatatt ctggaactaa ctcatcggta aaatgtaata
    13981 ccaccggagt aactgtaaaa acacggtact attatgctac tctaccatca gatgggtgta
    14041 caatggctac aggaacatct actgctgtat cacgtccaaa tttgtatgta accggtaaga
    14101 ccaccggacc tatcggcaca tcaggcaatt atttatattc agcattacca tgggttgttg
    14161 gaacgcctgc catacctgct ccttacgtgc atcatggcga tggcaccgtt accgcacaat
    14221 cgctctacga tgacaatact ttgttaagtg atcatgtatt cgattattat ttcgatggta
    14281 aggttgctga tcaggattta gggctgcatt ccgattataa aatggcatca cttgaagaga
    14341 tgatagcttt tatggaagag cagcgacatc tccccaccat tcaaggtcgc agtgcatgga
    14401 aacagaatgg caaattctcc gtcggccagc ttagcaccga actttgggaa acatctgaaa
    14461 cacagtcgct ctatattttg gaattaaaca agagaatcga agcattgaag gccaaatctg
    14521 attctctcga acccactaaa aagtaacagg catgacaaca cacttacgtg cttttatttt
    14581 tgctctgctt ttacttccgg caataactgt gttcggacaa ggagaggttg gctttggcac
    14641 agacacccct gctgaaaaag ccgatatcaa tggcgcgatg attattcgtg gcgctgcagt
    14701 tgctgcaaca cccattcttg gcacaatacg atggaacagt accgacggta tgcacgacgg
    14761 tcgcgaaact ccgggagtct ggacaagatt ggaaaatgat gaattcgaat ataacggcga
    14821 ctatactgcc atggtttgcg gaactacttc aaccacatca gcaggaaccg ctaccggaac
    14881 aagtactact gcatcagaaa caccatttgc tacggcttat agcgataaac gaagccaata
    14941 cttatattat ggaactgaga ttgcgctcgg cggactttgt gccggttata tcacacaaat
    15001 aggctttcag gtaagtgtgc ttggcgctcc ggcagctttg aacggtttaa ccgttagcat
    15061 gaaaatgaca actactacat cgctttcagg cttaacttgg gataccggac ttaccaccta
    15121 ttataatgga aattacacct taggccccgg acctaattat atttcactaa catcaggagc
    15181 atatccttcc ggattttatt gggatggtta ttctaatatc gaattggaat tatgttacga
    15241 taacgtttca agttcattta ataccactgt agaaatttct tccggattag catatacggc
    15301 aactcgtaca cattacgcca gtccgggtgt cggatgcgca cttacagcat cagtaactga
    15361 ggcaaaacac cccgtattat acctcacagg aacaggtgtt gggccaacta ccggagtggg
    15421 tgattatttg caatttaatt tgccaattat gattggctca cccgttctac ccgcacctta
    15481 tgtgcaccat ggtcccgggt caattacggc tgaagcaatt tatgatgaaa acgtaattat
    15541 cagcgattat gtgtttgata attatttcga cggtgaaatg tcggaaacag ataaagtaaa
    15601 acatgccgat tacacaatgc gcagtattga tgaaatgact gaatacatgc gcattcatcg
    15661 acacctgcca actatcagag gaagaaatga ttgggaagaa aatggaaaat tcagcaccgg
    15721 tgagcttgct tcagaattat gggtgactta tgaaacacat gccctttatc tcaaagaatt
    15781 acatgaacgc acacttgttg cagaacaatt attaatgacc tccgacgaga tattgaaaga
    15841 tgcgtatctc gcagaaatat tccgattgaa tcaagataac accttaacag aatcggtcaa
    15901 gtcagaaaga atcgcattgt tgaaccaaaa aattgaggaa ctgaaccatg cgaagtaata
    15961 gcaagataat gaaagggatt tatttagcag cgtttttatc ggttgcctct cttagtgcat
    16021 ttagtcaagg atacaccgga ataggtacta atactccgca agaacgtcta gatgttaatg
    16081 gggcaataat cgtcaggtct gatgctgttt cacctacacc tgttcccgga acaattcagt
    16141 ataatactac cgatggttat catgagggct atacaagtgc cggaacctgg ataaaattgg
    16201 aaaatgaaaa taccattggt tacggcgatt atatcactgt tgtagaaacg tgtacttctc
    16261 ctgccactgt tggaagcaat gaatatacaa cgccttcaac aggatatttt aatcccaact
    16321 atgaagaaaa tccgttttct acttggtgga tggatgacag aacacagtta ttatataagg
    16381 cttcgcaatt aaccgctgca ggcatctgtg cgggaaatat caatgccatc ggttttaacg
    16441 tcgtgactcc cggagcatat gccatgacaa gtcttactat taagatgaag tcgaccaata
    16501 ttactacttt gacagcctac gaaactgcat tgtcaactgt ttattccgcg ggctcttata
    16561 cacctgttgt tggcgcaaat gattttacac ttacctctcc attttattgg aatggaactg
    16621 acaatattgt aattgaagta tgttttaata actcagcatg ggcagcaggt tgtacagtta
    16681 ccggcgatat gaacgttgga tatcagtcgg ctgtcggtta ttacgccgat atcgcagctg
    16741 caggattgtg ttcagataat accacctatt ttacagatga tcttccgcaa ttgcgcgtca
    16801 ttggcgatgt gccaacacct actgccggaa caggttctta tattcaattc agtgatgccg
    16861 ttgtaattgg taatccaaca ttattttatg gtggtgatta taatggcccg ggaacattaa
    16921 cagtgctcgg tgcctatgat gataatacct tgatctctga ttatgtgttt gatgaatatt
    16981 tcgatggaaa aatatctgtc gaggatatta aaaaacatga taattataag cgatactcta
    17041 tttcggagat gagtgaattt gttgaaaaat atcgccatct ccctaccatt gaaggacgag
    17101 atgcatggga ggaaaaaggc ggtttctcac tcggagaatt gcttacacaa ctatgggtaa
    17161 cggcagaaaa tcaggctatt tatatttctg aattaaatac cgatttgaaa ttggttgaac
    17221 aggatatcat caataatcag gataatattc gcgcttcata caatcaagct ttaaatcgca
    17281 ttaattccga tgcattattg tcggaagaag taaaagcaat ggccgttcaa tccatcaacg
    17341 atagattgcg cttgctcgaa ttattaatta acgcacacaa accataacgc tcaccgtgaa
    17401 aagacacata ctcatatcat ctatgtgcct gttgacagcg atcaatatgc acgggcaatt
    17461 atacgtattg ggcaatggca caaacaaaga agtgtatgtc gacggcaccc tcgttggcga
    17521 accggctttg tatattaatg gatatctgaa caataatggt ggcgaagtat ataacgatgc
    17581 cggtgaaata gaacttaccg gcaactggga aaattatccc ggcgttgcag gctcctatga
    17641 atctaatggt atcgaacgct ttaccggcgc tgtgaatcag atcgtaaaag gaaccatgaa
    17701 cggtaccaca ggtaatatca accaatttta taacctacgt gtcgataaaa ctgctgcagg
    17761 acaatttgtt tccctgcaaa caaatgcaaa tgtcaatcct tcaggcactg taaactttga
    17821 atctaacgga ataattcgca cagatatcag ttcacatgga aataacggtt ctctgtatgc
    17881 ttatgaatta tatgtgcgca atacggcagt agcttccatg attggttatg ctacctcagg
    17941 aacaaataaa tatatcgaag gtcgcctgcg tcgtcaggtg agtggctcag gagattatta
    18001 tttcccgatt ggcatccagt cgacattttt agatggtgaa gaaccttttc atatcaactt
    18061 tgccagcgct ccggcagcaa atatcctgtc gtatgtgcag cccggtacga ttgatctgtt
    18121 aggaacagtc atgttttgcg acgtgggaac agaccctgat ccggctttgg gaactaccat
    18181 tctagataac cccggatcac ctccggatgg tatcctcgac cgactggaaa caaactgtca
    18241 gtattctgtt gaatgggtcg ctacggcaag cttagcgggt ccttatgatt atacgatcac
    18301 agttgaaccc ggttctgtgt tgcaggctga atgtccattc tattctgcta cctggttggg
    18361 tgaattgaaa tttacagcga aagacggaat tccaaataat gcagcattgg catcccctgc
    18421 accatttttc acaccgggct atatgacatg cccgaacttg tttacgattg cgggtttatc
    18481 cagcttttcc gtattccgtg tgcatggtat tactgatggt gctttggttg ttttacctat
    18541 cgaattattg gattttaccg gacagacaga atcgctcggc aatagattgt tctggactac
    18601 tgaaaatgaa actaacaacg attattttgt ggtagaaagt tctgccgatg cggctagttt
    18661 caaacaaatc ggaatcgttg atggagcagg aacttctact aatcggttgt cttacaattt
    18721 ccttgatgag catcctgtgg cgaataccac ttattatcgt ctgcgcaccg tagattttgc
    18781 cggtgcaata ggttatagta atacgatctc attaacacgc atgtcaaatg agcagttaag
    18841 tgtttcacct gtgccatcaa tgagttcagt taccgttgtt tttgcagctg atgcatcaca
    18901 agaggctata tttactatca ccggtatgga tggcaaggta atgctcaata agaagtggaa
    18961 tctcaaaccg ggccaaaatg agcagtctgt cgacatcact tcatggatgc cgggcaccta
    19021 tgttgttcag gttgatggcg tcaatgctcc attgcgcact aaattgatca aacaataagc
    19081 tgtcgaaggc gctttacagt atccttacaa gtcagtaatg gttacaatct atatctttac
    19141 caccggatat ggtaagtgag atattattga tattgctgtt agtcttgata aacggattac
    19201 tcgccatgag cgaaattgcc atggtgagtg ttaagaaagt tcgcctggaa tcggcggcta
    19261 aaagaggcga tattgccgct aaacgagcgc tacaactaca taatgaaccg gctaaatttc
    19321 tcagcaccgt tcagattggg ataaccctca tcagcatttt aacgggtatt ttttcaggcg
    19381 caacaatcaa attggcgttg gcagaggacc ttgccaaaat tcccgttatt gaaccatacg
    19441 ccgatactac ggcattggtt atagtagtaa ttgccattac ggcattatca ttgattgtgg
    19501 gggagctggt gccaaaacga ataggcatgc gattccccga accaattgca aaggcggttg
    19561 ccggaccgat gttactgtta tcggcaatta caaaaccttt tgtttggctg ctgacaaaag
    19621 ccagtgatgg aattatcaga attctgcgcc taaaggcttc gagtgaagat caggtgaccg
    19681 aggaagaaat taaagccatc attcaggaag gaacgcaaac aggggcaatc gacgaaatag
    19741 agcaggatat agtggagcgc gtttttcacc tgggcgacag gcgtattgcc agcctcatga
    19801 cccatcgcaa tgatattatc tggcttgatg taagtgaacc catcgaaagg gtttacgagg
    19861 atattcaaag tgaggtgcat agtatttatc cggtttgtga tggagagttc gataaggttg
    19921 taggtatcgt gtatattaaa gacttgttta ttgcaagaac caagaaagag attacaagcc
    19981 tgcgcgatct tattaaaccg gtattgtttc ttcctgaaaa taatacggct tatgatgtgc
    20041 ttgctaaatt caaggaatcg aaaattcatt atgcattggt ggttgacgaa tatggatcgt
    20101 cgcagggact cgttaccatc aatgatatcc tcgaagcact ggtaggtgaa tttgatgaat
    20161 tgaatcttcc tgattattca ataatcgaaa gagaagatgg ttcttatctt gttgatgcgg
    20221 ctcttccttt ttacgacttc ttgaaatatt tcgacatgga agaggaattc gaaatgctgg
    20281 atgaattaga gtataatacg ttggcaggat ttattcttca tcaattggaa catattccta
    20341 aagaaggcga acgcctcgat tggaagcaat atgaatttga aattgtagat atggatgcca
    20401 cacgcatcga taaggtaatg tttaccctca aataattttc atgattctga tagccgatag
    20461 cggatcttcc aaaacagatt gggccttgct gcatccggtg aacggcgacg tgcttccatt
    20521 gactacaaag ggattgaatc cgattgtgct tccggtcgac gcgttaataa gtatcatgtc
    20581 cgcaaatgag cacttattgg aatgtgctac ggatgtcagc gctgtttatt tttatggcgc
    20641 tggatgtaca ggtgatgtat caattggaaa tatgcatgca gcattggcaa gtatattcaa
    20701 atccgccaaa attgccgtgt tctccgatat gaccggtgcc gtgcatgctg ttgcagataa
    20761 taaaccttgt attgtttcaa ttttaggtac tggtgcaaat agttgtttgt ttaacggtaa
    20821 atctatttca ggtaatgatt tcagcctggg ttatgtgctg ggcgatgagg gttcaggcac
    20881 atggtttggc aagcgtttaa ttcgtgatca ctggtactgt tggatgccgg aaagcattgc
    20941 tgcggagttt gaaaaacaat atgcattaac aagagaagaa atattaaaac aaacatatcg
    21001 ctctccggca ccaaatgcat ggctggcttc gtttgcacca tttattgtgc aacacaggca
    21061 acatgaatat atgcaggaag tgattttcga agggttaacg gatttctttg atgcctacat
    21121 caacaggttt gaaaactcaa atgccttgcc gctacatttt atcggcagcc tgtcatgcgc
    21181 actggaacct gaaataagaa atgtggctga agaaaatgca ctttcttgcg gcaaattcat
    21241 ccagtcgccg atcagtggat taatacaatt ttaccaatca tctcattaat ctttgttatg
    21301 attccatcag aaagtttcag agatagtata atttataaag cgcttaccga tgatgccgaa
    21361 cgcttgaaac aaatgcacct caggcaattg tttcaagatg aagaacgctt cgcttccatg
    21421 tccatttttt gggatgagct gttatttgat ttttctaaaa acagaatcga cagtaaagca
    21481 tggaaaaatt tacatcgctt agccgaggaa atgcagctta ccgaagcttt tgaaggtatg
    21541 cttcgcggag ataaaataaa tgtaactgag ggtcgaccgg tattgcatac tgcattacgc
    21601 aatttttctg acagcgaagt attggtgaac ggaaaagacg ttatgcctga tgtgcgagag
    21661 gtgttgcaaa aaatgaaaat cttttcagat cagattattt cgggtgaaaa gaggggatat
    21721 accggtaaac aatttacaga tgtagtaaat attggtatcg gtggctcaga tcttggtccg
    21781 gtaatggttt gcgaagcact aaaacattat catgttccgc atcttaaagt gcatttcgtt
    21841 tcaaacgtcg atggatcaca tattacagag acaataaaaa atctgaatcc tgaaacaacc
    21901 ttgtttctta ttgcctccaa aacttttact acacaagaaa ccatggccaa tgcgcatacc
    21961 gcaaagcaat ggttcctgca tactgcacaa catgagcaac atgtggctgc acattttgtt
    22021 gccctgtcga ctgcggaaaa agaagttact gcattcggaa tcgacagaga aaatatgttt
    22081 gttttttggg attgggtagg agggaggtat tcactctggt cggctatcgg tttgtcgatt
    22141 tgtcttgcca tcggttatga gttgtttgaa gatttattgt atggtgctca tcaggtcgac
    22201 aatcttacga agtcagtaga accggagcat aatattccgt tgaccatggc cttattgggt
    22261 gtttggtata acgaatggtt tgatgcacat acccatgcgg tgattcctta cgatcaatac
    22321 ctgcatcgct tcacggcatt tttgcaacag gcggacatgg agagcaatgg caaatcggtt
    22381 caacgcaacg gtaagcgtgt ggattatgct acgggacctg tggtgtgggg tgaaccaggg
    22441 acgaatggcc aacatgcttt ttttcaactc atgcatcaag gcacacaatt aatcccggcc
    22501 gattttattg ttgctgcaac gcctttgcat aacatcatgg aacagcatca tatgttgctg
    22561 agtaattgcc tggcacaaac agaagcattg atgaatggta aaaccgaaga agaagtgcgt
    22621 actgagcttc gtcagaaagg gatggataat gaaaccatca ataagcattt accatatcgt
    22681 gtgtttgaag gcaacaggcc tactaataca attgtgattg atcaacttac gccatataat
    22741 ttaggtaggt tgattgcgat ttatgaacac aaaatatttt atcagggtat attgtggaat
    22801 gtgttcagtt tcgatcaatg gggagtggag cttggaaaaa aattagctgc tgaaatatta
    22861 cctgagtgga caagcaaaga aacattgcgc caccacgatt caagtaccgg cggacttatt
    22921 cagtatatca gaaatttgtt gggttaataa gtgtaaatgc ctgcagaatc aattcgccat
    22981 caaatcgtga atcgtgagta gtgaatcgtg agtcgtgaat cgtggatcgt gagtggtgag
    23041 tttttttaaa tacccgatac caaaaacaca tcacaagcat gcattagtct agctactaga
    23101 tacccgccag aggcgaggcg tgcctggctc tagatactaa tatcgtgaat cgtgattcgt
    23161 gattcgtgag tcgtgaatcg taagtcgtga atttttttac ctgatacctg atacctgata
    23221 cttgatactt gatacctgat acctgatact tgatacctg
//
LOCUS       DAOQHY010000035        22960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_993_length_22960_cov_6.170661, whole genome shotgun sequence.
ACCESSION   DAOQHY010000035 DAOQHY010000000
VERSION     DAOQHY010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 22960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22960
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_993_length_22960_cov_6.170661"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1490)
                     /locus_tag="PLL28_04580"
     CDS             complement(<1..1490)
                     /locus_tag="PLL28_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68628.1"
                     /translation="MKSARYYIWLLIMTLLSATGNRNVCAQQVKVPHAQPIKFIENKS
                     QWPEQVHFKGEFQGNSVYLEPTAFTFDLVDSRDLMEARHAHLSPNEPTPPVPEIHRHA
                     YRMQFIDANEQVQFATGNKSKEYYSYLLGNDPSKWGHQAYAYNDVTYQSLYQGIDIKV
                     YTSGENMKYDFIVKPGAKVEDIQIQYDGLIEVIAADGNLYLVTSIDTIVEMQPYAYQK
                     IGGRTIAIKCNFVVNGNQVHFEVEGNYNKNYELIIDPELVFASYTGSSADNWGYTATY
                     DEDGNLFGGGIAFGTGYPITVGAYMTSYQGGTVDISISKFTSDGTDLIYSTYLGGNSS
                     ELPHSLIATVTGELIIYGTTGSADFPITAGAYDNTFNGGAGVTVDGIVNFSSGIDIYV
                     AKLSADGTNLLASTYIGGTSNDGMNLATGLSTQYNYGDFARGEVIIDDANNVYVASST
                     ISSNFPTTPGAFQTTLGGDQEGIVFKFNPSLTTLAWSSFIGGNQEDG"
     gene            complement(1717..3555)
                     /gene="glmS"
                     /locus_tag="PLL28_04585"
     CDS             complement(1717..3555)
                     /gene="glmS"
                     /locus_tag="PLL28_04585"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNF68629.1"
                     /translation="MCGIVGYIGHREAYPVLIKGLHRLEYRGYDSAGVALINGDLKVY
                     KIAGKVSELEKVCEDQNTQGTLGIGHTRWATHGEPNTRNAHPHISESGNLAIIHNGII
                     ENYAPLKTELSKRGHKFLSDTDTEVLIHLIEDIQINEKLDLVEAVRVALNQVVGAYAI
                     VIVSKDEPDMLIAARKSSPMVVGVGDNETFVASDASPLIEYTRNVVYLNDEEIAIVRR
                     NKELEIITIHNENITPYMQELQMQLDMIEKGGYDHYMLKEIYEQPNSIQDSMRGRLIG
                     SRNAIVMGGIKEYEKKLVNAKRIIIIGCGTSWHAGLVGEYLFEDLARIPTEVEYASEF
                     RYRNPVITEDDIVVAISQSGETADTLAAIELAKSKGATIFGICNVVGSSIPRATHAGS
                     YTHAGPEIGVASTKAFTAQVTVLTLIALAIARKRGTITESKYQSLTVELSKIPEKVKQ
                     VLEANDHIKQIAEEYQNVHNFLYLGRGYNFPVALEGALKLKEISYIHAEGYPAAEMKH
                     GPIALIDENMPVVVMATNMSAHDKIVSNIQEIRARKGRIIAIISENDNVIETMAEHVI
                     RIPETEEALTPLLSVVPLQLLAYHIAVMLGRNVDQPRNLAKSVTVE"
     gene            complement(3559..4911)
                     /locus_tag="PLL28_04590"
     CDS             complement(3559..4911)
                     /locus_tag="PLL28_04590"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HNF68630.1"
                     /translation="MNRALTLLMCGCIVILTSCDKDNFIGKDLLPDEDFLNSEQIDTF
                     KIITYTDFDDSVVTSQNVFYALGSLDNQRYGKATASIYSQVLLPANNLFFGDAPEVDS
                     VVLTLDYSGYYGDTTLYHSLNVYRMSEPMQSGRNYHSDDKFRHIPVPIGRKSNFKVNM
                     LDSVMLADSSLWEPHLRIPMYNTFGTNIINLDSTILASDTSFLEYLQGIYIAPDTSAG
                     YSNAIMYFDMASDISGLRIYYHNSEADSLSVMLPLTGTKCNSFTNQLHPGTDIGDAIM
                     NADTTDGDAFTYVKGFAGARTIVKLPTLTNLQDVSINKAELVLTMTNDGSRYYPAPPK
                     VQLVQLDSTGRNYYYLALYTYELYSSIVDDNFGSTNIGGVAIKTTRDNTGRTVYEYQY
                     NITKHVQEIIEGSLDNNGFALICYPTNRVPNAVTLGGPMCPREDFKPRFTVTYTTINK
                     "
     gene            complement(4939..5748)
                     /locus_tag="PLL28_04595"
     CDS             complement(4939..5748)
                     /locus_tag="PLL28_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNF68631.1"
                     /translation="MEKKRVLIITQEMQPYHNGTDIGDIARELPQYVQDQGNEIRVLM
                     PRYGTINERRHRLHEVVRLSGMNIIINDDDYPLIIKVASVPGARMQVYFLDNDDLFKR
                     KHVFHDDNNKFFEDNAERMVFFCKAALETVKKFQWAPDIIHCHGWMTSLVPLYIKTTY
                     KNEPVFQNAKVVYSMYKGAFDEKLAKDKFLEKAPISTHIEADHLNEYKEYNHIGLSKG
                     GAKYADGVIIGSEDLEKELGKYAKSLGIPVLEYAKESYLEKYAAFYNELYQ"
     gene            5921..6817
                     /gene="panC"
                     /locus_tag="PLL28_04600"
     CDS             5921..6817
                     /gene="panC"
                     /locus_tag="PLL28_04600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNF68632.1"
                     /translation="MQVITTRSALTPLLLREKALGRKIGFVPTMGALHDGHLSLIAES
                     RKRADITVSSVFVNPTQFNDPKDLAKYPRQPEKDAAMLEKAGCDYLYLPAVEDVYPEG
                     PGKGPFFDLGGIDTRLEGASRPGHFAGVAEVVKVLVETLVPDFLFLGQKDFQQCMVVK
                     RMLDQWDKHHWDSALQEASLNWRPGDVELVICPIIREAHGLAMSSRNERLSGEMRTRA
                     SAIYASIQYAEELFPTFSADEISTICANALNSIPDFQVDYFQILDGETLSPANADTQK
                     FVIITAVIVAGVRLLDNKIIIN"
     gene            complement(6840..7604)
                     /locus_tag="PLL28_04605"
     CDS             complement(6840..7604)
                     /locus_tag="PLL28_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68633.1"
                     /translation="MEYPFEQRYKNFSIDELLEVIQFPDDYVPEAVMAAKAELSLRQL
                     TDEQLAEAQSVLDERKSQIRKKQELKQRQMEVVSDALDSIAREFIPGQTEPQRGDRLV
                     KIIGIICSIMVLAALYQNRYLIQMVSRDGLMLVDAFASLLPLVIWIIAIIFFFMRKKA
                     GWVMIGIIIAATAGLMIGQLILSPLAPEPFIQYTDSGQSNATLLLGTVLILALNYGLM
                     RMEVREIFNVSTSLKNKIIITFSALGFLLAGVFILG"
     gene            complement(7729..8091)
                     /locus_tag="PLL28_04610"
     CDS             complement(7729..8091)
                     /locus_tag="PLL28_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNF68634.1"
                     /translation="MRKLLAALSVCLILASCGGSGSKPSADAVKNLETAKRLEGKALL
                     TKNDCLTCHIEQEKLVGPAYKDVAAKYAGQEGAANMLVDKVINGGSGVWGDVAMAAHP
                     NLSRSDAEKMVNYILTVK"
     gene            complement(8199..9134)
                     /locus_tag="PLL28_04615"
     CDS             complement(8199..9134)
                     /locus_tag="PLL28_04615"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF68635.1"
                     /translation="MKTLCFLVAMGMLISLSAQTPLNKASECFDLYDAKQYQAALKCA
                     DGKIKNDSMLVEAYIAKGMALIELKKYQEAYDAYSLGIQRTGVKNELINHRGVFLLSL
                     QEFDAAIKDLSIAYEYAPDDTLKRFALVNRAAAYMSIRQFEMAYDDLLAAVKLDSSDV
                     SAYINLGVIADEIGKGELTLKYYEKALSLDSTQMMVYVNIGFKYQLDGQHDKAIKCFN
                     KVLEADPNDGVAYNNRGYSKMMTGDLKGAMEDVNKAIKLYPANSYAYRNRALIYLRMD
                     KQDKACDDITEALNKGFTTMYGDEMEKLYNKNCLK"
     gene            complement(9201..11408)
                     /locus_tag="PLL28_04620"
     CDS             complement(9201..11408)
                     /locus_tag="PLL28_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNF68636.1"
                     /translation="MRAFVFFLLIISNCSKAFTGVVHYDLHITDTTLYFNGKSVRAIA
                     VNGQVPMPTLVFTEGDTAEISVFNHLTESTSLHWHGVFLPNAEDGVPFLTQLPIEPGA
                     TYTYRFPVIQNGTHWYHSHSGLQEQIGMYGMLIFNKRSDDAQIRTGIDDIQNQEIILS
                     EWTTMHPNYIHHLLRGGSDWFTIKKHAAQSYAEAIGNHALGVKLSNEWKRMNAMDVSD
                     VFYDDILVNGQTSFQLLPADSLLRLRIANGSASTYYWLSYAGGKVMVIANDGNDVEPV
                     EVDRMLIAVSETYDIIVKIPTGHTYEFLVMSEDRSQSASIWLGSGDTIFAYKWPKLSY
                     FEGMKMMNGMMRMNGTMDAMGMRMGMQVMDMNEVMYPELNQQENVAIDHIHEVDEMQN
                     THTIGFPQTLNYSMLKATTNTAITGYDSIKTLHFTLTGNMQRYVWSINGKVVSEADKI
                     LIHKNELVRIVLTNNSMMRHPMHLHGHDFRILNGQGNYAPLKNVLDIMPMETDTIEFL
                     ANADGDWYFHCHLLYHMMSGMGRVFTIEDDTDTLRFIQNAYSRRKFYNEDKALHFMAD
                     GFLANNGSHGMAMIQNTRWQFSADWMVPFNPHPTYESEIHLGRFIGSNQWFMPYIGLM
                     YKSGRFGHDETMENIFGQNIIDRDPLLPAIGFTYLIPFNFTLQSDIMLDGSLRVQLMR
                     DDIPITPRLKLFVMGNTDMEYEVGLKYVIKPYLGLSVNYGNMMGAGAGLSFTY"
     gene            complement(11405..11887)
                     /locus_tag="PLL28_04625"
     CDS             complement(11405..11887)
                     /locus_tag="PLL28_04625"
                     /inference="COORDINATES: protein motif:HMM:NF023255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3347 domain-containing protein"
                     /protein_id="HNF68637.1"
                     /translation="MKSFILFSLIAIIHSISIAQGVSAGQILNRYMVVKEALSEGNYV
                     QANQAGALLKAALFVYDDSSETEKTAFLENVSEMYKYASKISSSSDLNEIRNAFAKLT
                     PAVWNFVSSSPAFNLNYNYLYCTMQKAYWIDYDSSVINPYVDKSMQECGVIRNLNVLQ
                     "
     gene            complement(11895..12131)
                     /locus_tag="PLL28_04630"
     CDS             complement(11895..12131)
                     /locus_tag="PLL28_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68638.1"
                     /translation="MENQIDKMVITNTFKTNINCSGCVARVKIELEKLTELSHWSVNT
                     MVPDKFLSVTAPEARMEEVISAVKRAGFNIEKIK"
     gene            complement(12206..14629)
                     /locus_tag="PLL28_04635"
     CDS             complement(12206..14629)
                     /locus_tag="PLL28_04635"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNF68639.1"
                     /translation="MANEIEEPIILPIEGMESEHCALLIDRELVKLPGIDQVQIELNN
                     KRALIKTTNAPDVIKAGVERIRGIGYDVVTITQSIPVLQMTCASCAISVESIVKAQPG
                     VIKSVVNFADASLLVEYIPTIINPQQIRAAVQSIGYDLLIVDANVEDTLEQIQQKNYL
                     RLFNRTAGAVVLSIPLILISMVFMDMPRANYLMWLLSTPVVLWFGRSFFVNAWKQAIH
                     RKANMDTLVALSTGISYIFSVFNTVNPAFWTSRGLDSHVYFETASVIIAFILLGKLLE
                     ERAKGKTASAIKKLIGLQESSVQVLQKDGSVRNEQINNVQHDEIIVIKPGAKIPLDGI
                     ITAGESFVDERALTGEPIPVFKSKDDKVFAGSLNGSAVLKVKVTHKGSETMLAQIISM
                     VRNAQGSKAPVQKLVDKIAAIFVPIVMLLALLSGLLWWVLGGENGLTQGLLAMTTVLV
                     IACPCALGLATPTAIMVGVGRGAANGILIKDAASLEQARDITAVVLDKTGTITRGEPK
                     VSEIIWFDDNPIYKQLLFTIEKYSSHPLANAIVQSMPESSILALETSTAVGGKGMIAK
                     YQEKNWFVGNSKLIQENKVSISDTLMVKANELVSQGSSIVWFTDENQVYALIAISDEI
                     KSSSKMAISQLKEMGIEVHMLSGDNQISAESIAHEVGITYVKAGVLPAEKATYIKKLQ
                     EAGKKVAMVGDGINDTAALAQSDVSIAMGAGSDIALDVAKITLMSSDLLKVPAAIKLS
                     VKTVRAIRQNLFWAFIYNIIGIPIAAGILIPITGFQLNPMLAAAAMALSSISVVGNSL
                     RLKWSHIGM"
     gene            complement(14710..15270)
                     /locus_tag="PLL28_04640"
     CDS             complement(14710..15270)
                     /locus_tag="PLL28_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNF68640.1"
                     /translation="MKIYIRNMVCARCKMVVSGVLTELGYKPVVVELGEVEISGDVDS
                     EGKDKINNRLKQFGFELIDDKRSKIIDRVKTLVVDIVHNKDGDLTTNLSDLLSNELHH
                     EYNYISNLFSSVEGTTIEKYFIAQKIERVKELIIYDELSLSEISFRMHYSSVAHLSSQ
                     FKKVTGLTPSEFKAINGPRRTNIDEV"
     gene            complement(15305..17017)
                     /locus_tag="PLL28_04645"
     CDS             complement(15305..17017)
                     /locus_tag="PLL28_04645"
                     /inference="COORDINATES: protein motif:HMM:NF019351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNF68641.1"
                     /translation="MKIKLICVCSLVMIKLSALTGQNALQIPPALYGPVYDLNVQNGV
                     VEFIPGITTPTYGINGNILGPTLFADKGDVVTLNVTNSLTGTGNSTTMHWHGFHLPAS
                     ADGGPHQVIDQGDTWSPSFTVMNDASTLWYHPHGDGKTDLQVARGLAGFFIIKDEVEN
                     ALTLPRTYGVDDIPLAIQTKAFDVLHQIAIATEMDTLPMVNATVDAVADLPAQVVRLR
                     LLNGSSMRTFHIGFDNNQLFYVIAGDGGLLSEPVALNRLILAPGERYEILVDLSGMES
                     EVLTMMSFGSTLPSGMYGAANPSGMGMGTITGYDANPLNGSDFSLLKIQVIDQTADPV
                     TEIPSSLTPPITTPAYTATRVFNLEPESMGMDNMVMGPFVINGASFNMDIVNEIVPIN
                     TIEKWRINNNTGIAHPFHIHEGQFIIDNINGAEPDPVDRGMQDVVYIPAGQYAEVIKT
                     FSDFADDQVPYMYHCHMLHHEDDGMMGSFTVVAQDEVINDHIVPPLIYPNPVASNSTF
                     SIVTEGEFERMEIYNSMGEIVYLDDFHQQAVNAYTIDGLPSGVFIIKLINGKNDNATT
                     FIVQ"
     gene            complement(17028..17810)
                     /locus_tag="PLL28_04650"
     CDS             complement(17028..17810)
                     /locus_tag="PLL28_04650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68642.1"
                     /translation="MKKSLAFCIMLMFAMHFNGIAQTAFQFTGEDCNGNAVDLYSDLD
                     AGKAAILLFYMPDCGACPPPASEIQEMANNVMNDFPDMIKGYVFPFLDIYDCPSVVSW
                     VDDSHVPFFTPMDSGEYQVAYYGGFGMPTVVLLGGTDHRVMFSTLSYLTSDTTIMKDS
                     ILRLFGVETAIEPVLDELKDLIVRPNPAYALISVSAAEMPIGEYVLTLVDINGAVMLN
                     QMGVSDGKLNATLDIAEISSGLYMLNITGANMHVSKSIMISH"
     gene            complement(17825..18694)
                     /locus_tag="PLL28_04655"
     CDS             complement(17825..18694)
                     /locus_tag="PLL28_04655"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68643.1"
                     /translation="MQFKNFITVIFSSLAIVSSAQIPNYSFEDWTTIDSYSMPVQWDC
                     LNALTETAAMFTCEQGTPGSPGSSFLKLTSKNIPGMGVMPGVAVSGVIDTITYAPISG
                     FAFTDRPESLTGKWQHMIYGGSQGYIDVVLTRWDLTSGERVTVASAHKQLTGMAMSWA
                     SFSIPLDYADDNFPDSCIITLRASGNSPSQNDFLYIDNLQFEGTVVSTGVINHNSTLQ
                     IFPNPAHDVLYISDASDLDFDDIVVVDAIGNICHVSTSLQGNNKIMLDCSGLTPGIYV
                     AHILSENIKFLVN"
     gene            complement(18761..19153)
                     /locus_tag="PLL28_04660"
     CDS             complement(18761..19153)
                     /locus_tag="PLL28_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68644.1"
                     /translation="MVTGKHLRLPIFLVLAFFAYSYVCSGYCQITAAISPSHDDEMAD
                     CCAKASPDQPTDEGNCQTKHIVFYNITGQFFVDHQVIIHPDINIQLEVSEIVPLPQAD
                     ISRLVEITNTGPPFSGAFTRIKVSSFQI"
     gene            complement(19301..20530)
                     /locus_tag="PLL28_04665"
     CDS             complement(19301..20530)
                     /locus_tag="PLL28_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68645.1"
                     /translation="MITSQAVIVVCILLLLAYIIDISAKHTKIPSVILLLALGFGVGR
                     LTQWLYIPVADLEPILPVLGTIGLILIVLEGALDLELNNSKFPAIRLALVNALLPILL
                     LAAIITTMFMFFWQLPLRVALVNAIPFCVISSAIAIPSVRGLSVFQREFVIFETSLSD
                     IIGVLFFNFILYNEVIDGHAFGHFGLQMLLIIVISFLAVLCLSFLMAKLSHHVTYTPI
                     LILIILIYYVSKIYHLPGLIFILVFGLFLGNLDEIKKFRWVEKFNPAKLDAEVHKFKS
                     ITLEATFIVRVLFFMVFGFLLKPEEIINLQTLPFAAGIVGLILLIRYVSLRISRLPIQ
                     PLLFIAPRGLITILLFISITGKLTSSHVTSAMIVQTVLLSVIIMMLGLMFYEPVEQNV
                     GTVTNKEARPNAHEAHE"
     gene            20823..21173
                     /locus_tag="PLL28_04670"
     CDS             20823..21173
                     /locus_tag="PLL28_04670"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNF68646.1"
                     /translation="MRVEVLKSKIHRVRVTESNLNYIGSITIDEDLLDAANMIEFEKV
                     QVLNINNGARLETYIIKGERGSGVICLNGPAAHHVTPGDIVIVCSYASMEWEEAKTWQ
                     PTLIFPDSQTNMLR"
     gene            21175..22206
                     /locus_tag="PLL28_04675"
     CDS             21175..22206
                     /locus_tag="PLL28_04675"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNF68647.1"
                     /translation="MSRKLIFNILKFAIFLGIGVTLIWYITKDLTADQKHELWASLSA
                     ANYWWVGFSVLIGIASHIARALRWKMMLEPLGTNPRLSTTFHAVMIGYLANMAVPRLG
                     EITRCGIVQRYEKVPFDKAVGTLVVERSIDLLCLIIATGILFITQFTLIFDFFDEKVI
                     FPISQKLQFSTAFIIILAAIIAAISFGIWFFIKKFSHTEAYLRIRILILNVRDGIYSI
                     RHMKNFKLFLFYTFFIWFCYFSSAWVALYALSQTSGFGPNEALTILVFGTVGIISTPG
                     GIGAYHLIVTETLVALYGLDRTYAISFSWLAWSCQTIMIIFVALISLLAISRLSKPQQ
                     DEQASGHTA"
     gene            22178..22672
                     /gene="rfaE2"
                     /locus_tag="PLL28_04680"
     CDS             22178..22672
                     /gene="rfaE2"
                     /locus_tag="PLL28_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HNF68648.1"
                     /translation="MSKPLAIQHKIMNLHTLVQQISVWRFSNRKIVFTNGCFDVLHKG
                     HAHVLNTASNLAYNAAVIVGVNSDASVKRLKGNDRPVNNEADRMLMLASMYAVDAVVL
                     FHEDTPYELINAIQPDFLVKGGDYAEDQIVGADIVKSRGGKVVVVPFLDNYSTTLLIE
                     KMKH"
BASE COUNT         6797 a         4950 c         4358 g         6855 t
ORIGIN      
        1 ccatcttcct ggtttccgcc aataaacgaa ctccatgcaa gggtggttag cgatggatta
       61 aatttaaata ctataccttc ctgatcgccg cctaatgttg tttgaaatgc gccgggcgtt
      121 gtagggaaat ttgaacttat tgtactactt gctacatata cattgttggc gtcgtctata
      181 attacttcac cgcgcgcaaa atctccataa ttatattgtg tgctcaaacc tgttgccaga
      241 ttcatgccat catttgatgt tccgccaatg tatgttgatg ccaataaatt tgttccatcg
      301 gcacttagct tggcaacata aatatcgata cctgaagaaa aattcacgat gccatcaact
      361 gttacacctg cgccgccatt aaatgtatta tcatatgctc cggcagtgat tgggaaatcg
      421 gcactacctg tagtaccata aataatcaat tcaccggtta cggttgcaat taaactgtgt
      481 ggcaactcgg aactatttcc tcctaaatat gtgctataaa taaggtcagt tccatcagat
      541 gtgaatttcg atatactaat atcaacagtt ccaccctgat aactggtcat atatgcgcct
      601 acggtgattg gataccctgt tccgaaagct atacctccac cgaataaatt tccatcctca
      661 tcataagtag cagtatatcc ccagttgtcg gcacttgatc ctgtgtaaga agcaaatact
      721 aattccgggt caataatcag ttcgtaattc ttattgtaat tcccttcaac ttcgaaatgc
      781 acctgattgc cattaacaac gaaattgcat ttaattgcaa tagtgcggcc accgattttt
      841 tgatatgcat aaggttgcat ttcaacaatg gtgtcaattg aagttaccag atacagattc
      901 ccatctgccg caattacctc tattaatcca tcatactgaa tttgaatatc ttcaactttc
      961 gcaccgggct tcacgatgaa atcatatttc atattttcac ctgaggtata gacctttatg
     1021 tctattccct gatacagcga ttgataggtt acatcgttat atgcataagc ctgatgtccc
     1081 catttcgaag gatcgtttcc taataaatac gagtaatatt cttttgattt atttcccgtt
     1141 gcaaactgta cttgctcatt cgcatctata aattgcatgc gataagcgtg tcgatgaatt
     1201 tcaggcacag gaggtgttgg ttcattagga ctcaaatggg catgacgtgc ttccatcagg
     1261 tcgcgcgagt caacaaggtc gaaagtgaat gctgttggct cgaggtaaac ggaatttccc
     1321 tgaaactcgc ctttaaagtg tacctgttca ggccattgag atttgttttc aataaatttt
     1381 atgggttgtg catgcggcac cttgacttgt tgggcacaca cattcctatt gcctgttgcc
     1441 gacagcaatg tcatgatgag gagccatatg tagtaacgtg ccgatttcac gattattcgg
     1501 tttgttcctt gagcaaaaaa acgccattgc aacagaggtt acaacgacag atttttgcga
     1561 ctttcgccac aataccactt taaaaatact aaaaagcgtc ggatattctt ccgaactcgt
     1621 ggctcaatcc tgcctaaaaa gcaccttttc agccataata gcacgaaaaa gcactaaaaa
     1681 tgagatgttt ctgaccgaat ttgggttgta tgagacttat tctaccgtta cagatttggc
     1741 aaggttacgc ggttggtcga cattgcggcc gagcattact gcgatatgat aagccaacaa
     1801 ttgcaaaggc acaacgctga gtaaaggggt aagtgcctct tccgtttcag gaatgcggat
     1861 cacgtgttct gccatcgttt cgattacgtt atcgttttca gaaatgatgg caatgatgcg
     1921 tccttttctt gcgcgtatct cttgaatatt acttacaatc ttatcatgtg cactcatatt
     1981 ggtagccatt accacaaccg gcatgttctc atcgatgagt gcgatgggac catgtttcat
     2041 ttccgcagcg ggataacctt cagcatgaat atatgatatc tctttaagtt tcaacgcacc
     2101 ttccagcgcc accgggaaat tatatccgcg accgagataa aggaaattat gtacgttttg
     2161 gtattcttca gctatctgtt tgatatggtc attcgcttca agcacttgtt ttaccttttc
     2221 aggtatttta ctcaattcaa cagtaagcga ttgatattta ctttcggtaa tagtgcctct
     2281 ttttctggca atggcaagtg ctattagtgt aagtacagta acctgcgcgg tgaatgcttt
     2341 ggtactggca acaccaattt ccggaccagc atgcgtatat gatcctgcat gtgttgcgcg
     2401 cggaatgctg gaacccacta cattacagat gccgaatatt gttgcacctt ttgattttgc
     2461 caattcaatt gcagcaagtg tgtcggcagt ttcaccactt tgggaaatag ccacaacaat
     2521 atcgtcttcg gtgataacag gattgcgata tcggaattca gaagcatatt ccacttctgt
     2581 tggtatacgc gcaagatctt caaataaata ttcgccaaca agtcctgcat gccagcttgt
     2641 tccacaacca atgatgatga tgcgtttggc attaacgagt ttcttttcat attctttaat
     2701 acctcccatc acaatagcat tgcgtgaacc tatcaaacga ccacgcatgg aatcttgaat
     2761 agaatttggt tgctcgtaaa tttctttcaa catataatga tcatatccac ccttttcaat
     2821 catatccagt tgcatttgca attcctgcat ataaggggtg atattttcat tatgtatggt
     2881 aataatttct aattctttat ttctgcgaac gatggcaatt tcttcatcat tcaaatacac
     2941 cacgttgcgt gtgtattcaa ttaagggact ggcatcagag gcaacgaatg tttcattatc
     3001 gcccacgccc accaccatag gagatgattt tcgtgctgcg ataagcatat caggttcatc
     3061 tttcgaaacg atgacaatgg catatgcacc aacaacctga tttagtgcta cacgtacagc
     3121 ttcaacaaga tctaatttct cgttgatttg aatatcttct atcaggtgga tgagtacttc
     3181 tgtatcggta tcggaaagaa atttgtgacc ccgcttactt aattctgttt tcagcggtgc
     3241 gtaattctct ataatgccat tgtggatgat ggccaggttt ccgctttcgc tgatatgcgg
     3301 gtgggcgttg cgcgtattag gttcgccgtg agttgcccaa cgtgtgtgac cgatgccaag
     3361 agtgccttgc gtattttgat cttcgcaaac tttctcaagc tccgatactt tccctgcaat
     3421 cttatatact ttcagatcgc cattgatcaa tgctactcct gcactgtcat agccgcgata
     3481 ttcgaggcga tgcaggcctt tgatcagaac cggatatgct tcacggtgtc cgatatagcc
     3541 aacgatacca cacatgtttt atttgtttat ggttgtatat gtaacggtaa aacgaggttt
     3601 aaaatcctca cgcggacaca ttggccctcc taaagttaca gcattcggca cacgattggt
     3661 gggataacaa attaatgcaa acccattgtt atcgagtgaa ccttcaataa tctcctgcac
     3721 gtgcttggtg atattgtatt gatattcata aactgttcgc ccggtattgt cgcgcgtagt
     3781 ttttattgca acgccgccaa tattcgttga accaaaatta tcatcaacga tagagctata
     3841 taattcgtag gtatagagag cgaggtaata ataattacga ccggtagaat ccagttgcac
     3901 caattgcact tttggcggtg caggataata acgcgatcca tcgttggtca tggtgagtac
     3961 caattccgct ttattaatag acacatcctg cagattggtg agagtcggta atttcacaat
     4021 tgttctggct cctgcaaaac cttttacata ggtaaatgca tcgccgtcag tggtatcggc
     4081 attcatgata gcatcgccga tatctgtgcc gggatgtagc tgattcgtga aactattgca
     4141 ttttgttccg gtaagtggca acatcacact taatgaatct gcttcagaat tatgataata
     4201 aatgcgcaat cctgaaatat cggaagccat atcaaaatac atgatagcat ttgaataacc
     4261 ggcagaagta tcaggtgcga tataaattcc ctgaaggtat tcgaggaatg aagtatctga
     4321 tgcaagaatt gtggaatcca gattaatgat attcgttccg aaggtgttat acatcggaat
     4381 gcgcaagtgc ggttcccata atgaactgtc ggccagcatt acactgtcga gcatattcac
     4441 cttgaaatta gatttacgcc caatcggcac agggatatgg cggaatttat cgtcgctgtg
     4501 ataatttctt cccgactgca tgggctccga catacgataa acattcagac tatggtagag
     4561 tgtagtatct ccataatatc cggaataatc gagtgtcaat actaccgaat ccacttcagg
     4621 tgcatcgccg aagaagaggt tattggcagg aagaaggact tgtgaataga tgcttgccgt
     4681 agcttttcca taacgctgat tgtcgagcga gcctaaagcg tagaatacat tctggctggt
     4741 caccaccgaa tcatcgaaat cggtataggt gataattttg aaggtatcta tctgttcgga
     4801 attgaggaaa tcctcatccg gcaacaggtc ctttccaata aaattgtctt tgtcgcaaga
     4861 tgtcaggatg acaatacatc cacacatcaa cagagtcagc gcacggttca tcctgcctat
     4921 aacggagtaa atcacaaatt attgatagag ttcattataa aatgccgcat atttctcgag
     4981 atatgattct ttagcatatt caagtaccgg aatgcccaat gatttcgcgt atttgccgag
     5041 ttctttttcc aggtcttcgc tacctatgat cacgccatcg gcatatttgg caccaccttt
     5101 actgagtccg atatggttgt attctttgta ctcattgagg tgatctgctt cgatatgggt
     5161 gctgataggc gctttttcaa ggaatttatc ctttgccagt ttttcatcaa aagcaccttt
     5221 atacatcgag taaaccactt ttgcgttctg gaatacaggt tcgtttttgt aggtagtttt
     5281 gatatacagc ggcacgaggc tggtcatcca accgtggcaa tggatgatat ccggagccca
     5341 ttggaatttt ttcacagttt cgagtgctgc cttacaaaag aacaccatgc gttcggcgtt
     5401 gtcttcgaaa aacttgttgt tatcgtcgtg gaacacatgt ttgcgtttaa acaggtcgtc
     5461 gttatcgaga aaatacacct gcatacgcgc tccgggaaca gatgctacct taatgatcag
     5521 cgggtaatcg tcgtcgttga tgatgatatt cattccgctt aatcgcacca cttcatgcag
     5581 gcgatgacga cgttcattaa tagttccgta gcggggcatt aatactctga tttcatttcc
     5641 ctggtcctgc acgtattgtg ggagttcgcg ggcaatgtcg ccaatatcgg taccattatg
     5701 ataaggctgc atttcctggg taatgatcag cacacgcttc ttttccatag gcaaagatct
     5761 tgataagtcc tgcggggatc gtaactttcc ccgttctcct cagaaggtta tttttgaggt
     5821 gcaaagttac aaaatagtat tcagtattat attatgcaac ctaaagtgag ggtattttaa
     5881 caagtcttca ctttcggctg aaacccttat cattgcaagc atgcaggtca ttaccacacg
     5941 ttcagccctt acccctctcc tattgcgtga aaaggcactg gggcgcaaaa taggcttcgt
     6001 tcctaccatg ggagccttgc acgacgggca tttgagcctg atagccgaaa gtcgcaaacg
     6061 agccgatatt accgtttcca gcgtgtttgt caaccccaca cagttcaacg accctaagga
     6121 cctggccaag taccctcgac aacccgagaa agacgctgca atgctcgaaa aagcgggttg
     6181 cgactatcta taccttcccg ccgtagagga tgtttacccc gaaggacccg gaaaagggcc
     6241 ttttttcgac cttggtggca ttgatacccg cttagagggt gcctcacgac cgggacattt
     6301 cgccggggtg gccgaagtag tgaaggtgct ggtggaaacc ctggtcccgg atttcctgtt
     6361 tctgggtcag aaagattttc aacaatgcat ggtagtgaag aggatgctcg atcaatggga
     6421 taaacaccat tgggacagcg cgctacaaga ggctagtctc aactggcgac ccggagatgt
     6481 tgaattggtg atatgtccga ttataagaga agcacatgga ctggcaatgt cgtcgcgtaa
     6541 tgaacgacta tccggtgaaa tgcgcaccag ggcttcagcc atatatgcga gtattcaata
     6601 cgcggaggaa ttattcccta cattttctgc tgatgaaatc agcactattt gtgctaacgc
     6661 cttaaattcc atacctgatt tccaagtcga ttattttcaa attctggacg gtgaaacatt
     6721 atcaccggca aatgcagaca cacaaaaatt tgtaattatt acagcagtaa ttgttgcagg
     6781 agtcaggttg ttggataata aaattattat aaattgattt taatagcagt gagctatttt
     6841 caacctaaaa taaagacacc cgccaacaga aaacccagtg ccgaaaaagt gattataatc
     6901 ttatttttca aggaagtaga aacattgaat atctcgcgaa cttccatacg cattaaccca
     6961 taattaagtg caagaatcaa tacggttcca agcagcaatg tggcatttga ttgccctgaa
     7021 tctgtatatt gaataaatgg ttccggtgca aggggggata aaatcaattg tccgatcata
     7081 aggcctgctg ttgcagcaat aataatacct atcattaccc aacctgcctt ttttcgcatg
     7141 aaaaagaata tgatagcaat aatccaaatc accaaaggaa gcaaagaggc aaacgcatct
     7201 accaacatca gaccatcccg gcttaccatt tgaatcaagt aacgattttg atacaaagcc
     7261 gcaagcacca ttattgaaca aatgattccg attattttca ccagacgatc acctcgctgt
     7321 ggctctgttt gacccggaat aaattcgcgc gctatactat caagtgcatc ggaaacaact
     7381 tccatttggc gttgtttcaa ttcttgtttt ttccggattt gactttttct ttcatccaat
     7441 acagattgcg cctcagctaa ctgttcatcg gttaattgcc ttaatgacaa ttctgctttt
     7501 gcagccataa ccgcttctgg cacatagtca tcaggaaatt gaattacttc cagtaactcg
     7561 tcgatagaaa aattcttgta gcgttgctcg aaagggtatt ccataattgc ttgttgctaa
     7621 attaagaaaa ttagcgacta gcagcaatta ccatcctgga atggaaattt acttcgttcg
     7681 atataacaaa gccgtcaata aattgacgtg taaaaaaagc cttggaaatt atttcactgt
     7741 aaggatataa ttgaccattt tctcggcatc agacctactc agatttggat gtgctgccat
     7801 tgccacatca ccccaaacgc ctgaacctcc gttgattacc ttgtcgacca acatattcgc
     7861 cgcaccttcc tgacctgcat attttgctgc cacatctttg tatgcaggac caaccaattt
     7921 ttcctgttca atatgacagg tgaggcaatc attttttgtc aggagtgcct ttccttccaa
     7981 tcgtttagcg gtttcgagat tttttacggc atcggcactt ggtttagaac cactgccacc
     8041 gcatgaagcg aggataaggc atactgagag ggctgcaaga agtttacgca tatcggtaat
     8101 ttttacaaac acaaatgacc gacatactgt ggttttcgca aatgacctat actacttccg
     8161 acagcctgac atatgtcaat tgaaaaatga catacacact atttcaaaca atttttgtta
     8221 taaagctttt ccatttcatc accatacata gtagtaaaac ctttattgag tgcttcagta
     8281 atgtcgtcac atgctttgtc ttgtttatcc atgcgcagat aaattaatgc tctatttctg
     8341 taggcataag aatttgcggg atataatttg atagctttat tgacatcctc catagcacct
     8401 tttaaatcgc cggtcatcat tttgctgtat ccgcgattat tatatgcaac accatcgttt
     8461 ggatcggctt caagcacttt attaaaacat ttgattgctt tatcgtgctg tccatcgagc
     8521 tgatatttga atccgatatt tacatatacc atcatttggg tgctatcgag cgagagtgct
     8581 ttttcataat atttcaaagt aagttcaccc ttacctattt catctgcaat cacgccaagg
     8641 ttgatgtatg ccgacacatc gcttgaatct aatttaacgg cagccagcaa atcgtcgtat
     8701 gccatttcaa actggcgtat cgacatatat gcagcagctc tattcaccag cgcgaaacgt
     8761 ttaagtgtgt cgtcaggagc atattcatac gcaatagaca aatctttaat tgccgcatcg
     8821 aattcctgaa gagacaaaag gaatacaccc ctgtgattaa taagttcgtt tttaacacct
     8881 gttcgctgta tgccaagtga ataggcgtca tatgcttcct gatatttttt taattcaatc
     8941 aacgccatgc cttttgcgat ataggcttca accaacattg agtcgttctt gattttaccg
     9001 tctgcacatt ttaatgctgc ctgatattgt ttggcatcat agaggtcgaa gcattcggag
     9061 gctttattta acggagtttg cgcgctgaga gaaatgagca ttcccattgc aaccaagaaa
     9121 catagtgttt tcatgtttga gattttatac gaaaatatga gattccggtt agaatcgcaa
     9181 gttcatcaaa ttcagccaaa ttaataagtg aaggaaagtc cggcaccggc acccatcata
     9241 ttgccataat tcacagatag tcctaaatat ggttttatta catattttaa gcctacctca
     9301 tactccatat cggtatttcc catcacaaac agttttaagc gaggtgtaat aggaatatca
     9361 tctcgcatca gttgaacgcg caaagaaccg tctaacatga tatctgactg aagagtgaaa
     9421 ttaaatggaa taagatatgt aaatccaatt gcaggcaaca aagggtcccg atcaattata
     9481 ttctggccga aaatattctc cattgtttcg tcatgtccga atctgccaga tttgtacatt
     9541 aaccctatgt aaggcataaa ccattgatta ctcccaataa atctacccag atgtatttct
     9601 gattcatatg tcggatgagg gttgaaaggc accatccaat cagcactaaa ctgccaccga
     9661 gtgttttgaa tcattgccat accgtgactg ccgttatttg ccaaaaatcc atcagccatg
     9721 aaatgcagtg ctttatcctc attataaaat tttcgcctgc tataggcatt ctgaataaat
     9781 cttaatgtat cggtatcgtc ttcgatagta aacacccttc ccataccact catcatatga
     9841 tacagcaaat ggcagtgaaa ataccaatca ccatcagcat ttgccaaaaa ttcaatggta
     9901 tcggtttcca taggcataat atccagtaca ttcttaagcg gtgcatagtt tccttggcca
     9961 ttcaatattc gaaaatcgtg accatgcaaa tgcattggat gtcgcatcat agaattattt
    10021 gtaagcacta ttcggaccaa ttcatttttg tgtataagaa ttttatctgc ttctgaaaca
    10081 actttgccat taatagacca tacatatcgt tgcatattac cggtgagtgt aaaatgcaat
    10141 gttttaattg aatcatatcc tgtgatcgca gtattggttg tggccttaag catactatag
    10201 tttaaggttt ggggaaaacc gattgtatgg gtattttgca tttcatcaac ttcatggata
    10261 tgatcaatag caacattttc ttgttggttc aattccgggt acataacttc attcatatcc
    10321 ataacctgca tacccattcg catgcccata gcatccatgg tgccattcat gcgcatcata
    10381 ccattcatca ttttcatgcc ttcgaaatag ctgagcttag gccatttata ggcaaatatt
    10441 gtatcgcctg aacccagcca tatactcgct gattgggatc gatcttccga catcacaaga
    10501 aattcgtatg tatgcccggt cggaatttta acaatgatgt catacgtttc agaaactgca
    10561 atcagcatcc gatctacttc caccggttca acatcatttc cgtcattagc aatcaccata
    10621 acctttccac ccgcgtacga taaccaataa tatgtcgaag cagagccatt tgcaatcctc
    10681 aatcgaagta aagagtctgc aggtaataac tgaaaggatg tttgtccatt cacgagtatg
    10741 tcatcgtaaa aaacatcgct cacatccatt gcgttcatgc gcttccattc attagacaac
    10801 tttactccga gtgcatgatt acctattgcc tccgcatagc tttgcgcagc atgtttttta
    10861 attgtaaacc agtcagaacc acctctcaat aagtggtgaa tgtagtttgg atgcatagtt
    10921 gtccattcac ttaaaattat ttcctgattt tgaatgtcat caatacctgt tcttatttgc
    10981 gcatcgtctg accgtttgtt gaaaatgagc atgccataca ttcctatttg ttcctggaga
    11041 cctgagtgac tgtgatacca atgtgttcca ttttgaatga ccggaaaacg ataagtatag
    11101 gttgctcctg gttcaatagg caattgcgtc aggaaaggca cgccatcttc agcatttggc
    11161 aaaaataccc catgccaatg caaggaagta ctttcagtta aatgattgaa aactgaaatt
    11221 tctgccgtat caccttcagt aaaaacgaga gtaggcattg gaacctgacc attaaccgca
    11281 atagcgcgaa cagactttcc attaaagtaa agcgtggtat cggtaatatg cagatcataa
    11341 tgaaccactc ctgtaaaggc cttggagcaa tttgaaataa tcaaaaggaa gaaaacaaaa
    11401 gctctcattg taaaacgttt aaatttctta ttacaccaca ttcttgcata cttttatcta
    11461 cataggggtt tatgactgaa ctatcataat ctatccaata tgccttttgc atggtacaat
    11521 aaaggtaatt gtaatttaag ttgaatgcag gagatgaaga aacaaaattc caaacagcag
    11581 gagttaattt agcgaaagca tttcttatct catttaaatc ggaagatgat gaaattttac
    11641 ttgcatactt atacatttca gacacatttt caagaaaagc cgttttttca gtctcgcttg
    11701 aatcatcata aacaaataag gctgctttca acaaggctcc tgcttgatta gcctgcacgt
    11761 aattcccttc agacaatgct tctttaacaa ccatatatct attgagtatt tgtccggcac
    11821 tgacgccttg agcaattgag atgctatgta ttatcgcaat cagagaaaac aggataaagc
    11881 ttttcatacg acaattattt aattttttca atattgaatc ctgctctttt aaccgcagaa
    11941 atcacctctt ccattcgtgc ttcgggagca gtaacagata gaaatttatc ggggaccatc
    12001 gtattaacac tccagtgcga caactcggtg agcttttcta attcaatctt aacacgagcc
    12061 acgcaacccg aacagttaat attagtttta aatgtgtttg tgataaccat tttatcaatt
    12121 tgattttcca taatatttgt ttttgcaaag ttgattcagc aattgctgac acctattata
    12181 caatttatta ttttaattac atgatttaca tgccgatgtg cgaccactta agtctgaggc
    12241 tattgccgac aacactaatg ctactcaaag ccatagcagc agctgctaac atgggattta
    12301 gctgaaatcc cgtgattggt attaaaatcc ctgcagctat tggtattcca ataatattat
    12361 atataaatgc ccaaaaaaga ttttgcctta tggctctgac tgttttgacc gacaatttaa
    12421 ttgcggcggg aaccttcaat aaatcagaag acatcaatgt aattttagcg acatcaagtg
    12481 cgatatcact tccggctccc atggcaatgc taacatctga ctgagcaagt gctgcggtat
    12541 catttattcc atcgccaacc atagcaactt ttttaccggc ctcctgcaat tttttgatat
    12601 aggttgcttt ttcagcagga agcactcctg cctttacata ggttatacct acttcatgtg
    12661 caattgattc cgcagatatt tgattgtctc ctgaaagcat atgcacttca atgcccattt
    12721 ccttcaactg acttatagcc attttggaac tgcttttgat ttcatctgat atagcaatta
    12781 atgcatacac ttgattttca tcagtaaacc aaactattga actaccttga ctaactaatt
    12841 cattcgcctt taccatcagc gtgtcagata tactgacctt attctcttga attaatttac
    12901 tattcccaac aaaccaattt ttctcttgat actttgctat cattcccttc ccgccgactg
    12961 cagtgcttgt ttccaatgca agaatagaac tttcgggcat tgattgaaca attgcattgg
    13021 caagcggatg tgaagaatat ttttcaattg taaatagtaa ttgtttgtaa ataggattgt
    13081 catcaaacca aatgatttca gagacctttg gttcaccccg agtaattgtt ccggttttat
    13141 caagaactac agctgttatg tctctagctt gttcgaggct cgcggcatct ttaattaaaa
    13201 taccatttgc ggcacctctt ccaactccaa ccattattgc tgtaggtgtt gcaaggccaa
    13261 gtgcacatgg acaagctatt actaaaactg ttgtcattgc aagcaagccc tgagtaagcc
    13321 cgttttcgcc tccaagaacc caccatagca agcctgaaag cagggctaag agcataacaa
    13381 ttggaacaaa aatggctgca attttatcta caagcttttg cacgggggct ttgcttccct
    13441 gagcattacg caccattgaa attatttgtg ccagcatggt ttcagaaccc ttatgcgtaa
    13501 ctttcacctt caataccgcg gacccattca atgatccggc aaacacctta tcatctttag
    13561 atttaaatac cggtattggc tcaccggtta atgcgcgctc atcaacaaat gattctcctg
    13621 cagtaataat gccatccaag ggtatttttg ctcctggctt tattacgata atttcatcat
    13681 gctgtacatt gttaatttgt tcattacgga cagatccatc tttctgtaaa acctgaacag
    13741 atgattcctg caaaccaata agttttttta tagcagatgc tgtctttcct ttggctcgtt
    13801 cttcaagcaa tttgcctaat aaaataaaag caataatcac tgaagctgtt tcaaaataaa
    13861 catgggaatc gagaccgcgg gaggtccaaa aagcgggatt aactgtattg aatacactga
    13921 aaatatacga tatacctgta ctgagcgcca ccaacgtatc catatttgcc tttcggtgga
    13981 tagcctgctt ccatgcattc acaaaaaaac tacgaccaaa ccataatact acaggcgtgc
    14041 ttagcaacca catcaaatag ttagcccgag gcatatccat aaacaccatg ctgattagta
    14101 tcagaggaat ggatagcact actgcgccgg cggtgcggtt aaaaagacgg aggtagttct
    14161 tttgctgtat ttgttccaga gtatcttcaa cattagcatc aacaatcaac aaatcatatc
    14221 ctatagattg aaccgccgcc cggatttgct gtggatttat aatagtaggt atatactcaa
    14281 ccaatagtga agcatcagca aaatttacaa ctgactttat tacccccggc tgagctttaa
    14341 caatagattc cacgcttatt gcacaagatg cgcatgtcat ttgtaatacc ggaatacttt
    14401 gcgttatggt tactacatca taaccaattc cgcggatgcg ctctacgccg gcttttatca
    14461 catccggtgc atttgttgtt ttgatcagcg cccttttatt gttaagttca atctgaacct
    14521 gatcaatgcc gggtaatttg acaagttcgc gatcaatcag cagtgcacaa tgttcacttt
    14581 ccatgccttc aataggcaaa atgatcggct cttcaatttc gtttgccata gttgtatcga
    14641 tttaggatac aaagctgagg cgaattccgg gtccaagtgt tacactattt ctataatgaa
    14701 ttacatcatt tacacttcat cgatatttgt tcttctgggc ccgtttatag ctttaaactc
    14761 acttggtgta agtccagtaa ctttcttgaa ttgactactt aaatgtgcaa cagatgagta
    14821 atgcattcgg aaggatatct cgcttaatga aagctcatca taaataatca attcttttac
    14881 ccgttcaatc ttttgtgcaa taaaatactt ttcaatggta gttccttcaa ctgatgaaaa
    14941 taaattactg atataattat attcatgatg aagttcatta ctcagcaaat ctgacaagtt
    15001 agtagttaag tctccatctt tattatgcac tatatctacc accaaagttt ttactctatc
    15061 gattattttt gagcgtttgt catctatcaa ttcaaatccg aactgcttca atcgattgtt
    15121 tattttatcc tttccttccg aatcaacatc acccgatatc tccacctccc ccaattcaac
    15181 aacaacaggt ttatacccca actcagtcaa tactccggaa accaccattt tacatctggc
    15241 acaaaccata ttccgaatat atattttcat tatggcgtcg tttataataa tctaatttat
    15301 tatatcattg cactataaaa gtagtggcat tatcattttt accattaatc aatttaatga
    15361 tgaacactcc cgaaggcaga ccatcgatag tataggcatt aacagcctgc tgatgaaaat
    15421 catctaaata gacaatttca cccatagaat tataaatttc catcctttca aattcacctt
    15481 ctgtaactat tgaaaacgtt gagtttgatg caactggatt aggataaatc aatggtggca
    15541 cgatatggtc attaatgact tcatcctgtg ctacaaccgt aaaacttccc atcattccgt
    15601 cgtcctcatg gtgcaacatg tgacagtgat acatatatgg cacttgatca tcggcaaaat
    15661 cgctgaaggt tttaattact tcagcatatt gtccggcggg tatataaaca acatcctgca
    15721 taccccggtc aacagggtct ggctcagcgc cattaatatt atcaataata aactgacctt
    15781 catgaatatg aaagggatgt gctattccgg tattattatt aatgcgccat ttttcaatag
    15841 tattgatagg aactatttca tttacaatat ccatattaaa tgatgctcca tttattacaa
    15901 aaggccccat caccatgtta tccatcccca ttgattcagg ttctaaatta aaaactcggg
    15961 ttgccgtata agcaggcgtt gtaatcggcg gagtcaatga cgatggaatt tcagtgaccg
    16021 gatctgcagt ttgatcaatg acctgaattt taagtagtga aaaatctgaa ccattcaacg
    16081 gattcgcatc ataaccggta atagttccca tacccatacc ggaggggttt gcagcgccgt
    16141 acatgcctga tggaagcgta gaaccaaacg acatcattgt caaaacttca gattccattc
    16201 cactgaggtc aaccaatatt tcatagcgct cgccgggagc aagaattaat ctatttagag
    16261 ccacaggttc cgataataat ccaccatcgc cggcaattac ataaaataac tggttattgt
    16321 caaacccaat atgaaaggtg cgcatgctgg atccattcaa taatcgcaat cgcactacct
    16381 gagcagggag gtctgccacg gcgtcaacag ttgcatttac cattggcagc gtatccattt
    16441 ctgttgcaat ggctatttgg tgtaatacat caaatgcttt cgtttgtatt gcaagcggaa
    16501 tgtcgtcaac accataggtt cgtggtagcg tcaatgcatt ctcgacttca tccttaataa
    16561 taaaaaatcc cgccaatccc cgtgccacct gcaaatcagt tttaccatca ccatgagggt
    16621 gataccacaa tgttgaagca tcattcatta ccgtaaatga cgggctccag gtatctccct
    16681 gatcaatgac ctgatgtggt ccaccatcgg ctgatgccgg caaatgaaaa ccatgccagt
    16741 gcattgtagt tgaatttccg gttccggtga gcgaatttgt cacatttaat gttaccacat
    16801 cgcctttatc tgcaaataac gttggaccta atatattacc attaatgcca taagtgggtg
    16861 tggtaatgcc aggaataaac tcaactacac cgttttgcac attgagatcg tacaccggac
    16921 catataatgc aggtggaatt tgcaatgcat tttgccctgt tagcgctgat aatttaatca
    16981 tcactaaact gcagacacaa atcaatttaa ttttcatgct attccgttta atgactgatc
    17041 ataatcgatt tactgacatg catatttgct cctgtaatat ttagcatata caaaccggag
    17101 gaaatttcag caatatccaa tgtagcattt agttttccgt cactcacgcc catttgattt
    17161 aacattacag ctccattgat atcaaccagc gtcaaaacat actctccaat tggcatttca
    17221 gcagcgctga cagaaatcaa ggcgtaggca ggatttggac gcactatcaa atcttttaat
    17281 tcatcaagta caggttcaat tgccgtttca actccaaata atcgtaaaat agaatctttc
    17341 attatggtgg tgtcgcttgt caggtagctt aaggtactaa acatcaccct gtgatcggtg
    17401 ccgccaagca aaacaactgt tggcatgccg aagcccccat aataagccac ctgatattcg
    17461 ccactatcca taggtgtgaa aaatggcaca tggctgtcat ctacccatga aacgactgaa
    17521 ggacaatcat aaatgtcaag gaagggaaac acataacctt ttatcatgtc aggaaaatca
    17581 ttcatcacat tattagccat ttcctgtatt tcagaagcgg gtggcgggca cgctccgcaa
    17641 tcaggcatat aaaaaagcag aattgctgct ttacctgcat ccagatcact gtaaaggtca
    17701 actgcattgc cattgcagtc ttctccggta aattgaaaag ctgtttgagc aataccattg
    17761 aaatgcatgg cgaacatcaa cattatacaa aaagcgagac tctttttcat aaaaaatcaa
    17821 ttaattaatt tactaagaat ttaatatttt cagatagaat atgtgcaaca taaattccgg
    17881 gagttaagcc tgaacaatca agcattatct tattattacc ttgtaatgaa gtgctcacat
    17941 ggcaaatatt tccaatggca tcaacaacta caatgtcatc aaagtccaga tccgacgcat
    18001 cggagatata caacacatca tgcgccggat ttgggaaaat ttgcagtgtt gaattgtgat
    18061 taattacccc tgttgataca acagttccct cgaattgaag gttgtcgata tataaaaagt
    18121 cattttgcga cggtgaattt ccactggcac gcaaggtgat gatacaagaa tcaggaaaat
    18181 tatcatcagc ataatcaagt ggtattgaaa atgaagccca actcatagcc atgccggtca
    18241 attgtttgtg tgcagaagcc actgtaactc gttcgcctga ggttaagtcc caacgagtaa
    18301 gaacgacgtc aatatatccc tggcttccac cataaatcat atgctgccat ttcccggtaa
    18361 ggctttcagg tctgtcagta aatgcaaatc ctgaaatggg cgcataagta attgtatcaa
    18421 taacaccgct aacggcaacg cccggcataa cgcccattcc gggaatattt ttactggtga
    18481 gttttaaaaa actgctaccc ggtgagccgg gtgttccttg ttcacaagtg aacattgcag
    18541 cagtttcagt taatgcattc agacaatccc attgtacagg catggaatag ctatcaatag
    18601 ttgtccaatc ttcaaatgag tagttgggta tttgtgccga tgacacaata gctaaagagg
    18661 agaaaattac ggtgataaaa tttttgaatt gcataataag tagtttacag atgaaaaaat
    18721 aaatacccct tcttatgaaa aggaattact catctgaaaa tcaaatttga aaactactta
    18781 cttttattct tgtaaaagct ccgctgaacg gcggacctgt atttgttatt tctactaagc
    18841 gtgaaatatc cgcctgaggt aagggtacta tttcagatac ctccaattga atgttgatat
    18901 caggatgaat gattacctga tggtcaacaa aaaactggcc ggtaatatta taaaatacaa
    18961 tatgtttagt ttggcaattg ccctcatccg taggttggtc aggtgaagct ttagcacagc
    19021 aatctgccat ttcatcgtca tgtgatggac tgattgcggc tgttatctgg caatatccgg
    19081 aacacacgta gctgtaggca aaaaatgcca agaccaagaa aatcggcagt ctaagatgtt
    19141 tcccggttac cataagctat gtcaaaatta gcgaattgaa agtttgctac caaatacaat
    19201 ttcccccggc caacaaatat tgacttttgg agccatccta tggtaaataa cgagaaatac
    19261 aactgtatgc aagccaaaaa cgatcagtat ctctaggttg ctattcatga gcttcatgag
    19321 cgttggggcg ggcctcttta ttcgttaccg tgcctacatt ttgttcaaca ggctcgtaaa
    19381 acatcagtcc taacatcatg ataatcactg acaacagcac tgtttgtaca atcatagccg
    19441 acgtaacatg cgacgatgtt agttttccgg tgatcgaaat aaacaatagt atggtaatca
    19501 gccctcttgg tgcaataaat aaaagaggct gaatcggtaa acgggaaatc ctcaagctga
    19561 catatctaat caggagtatt aatcccacta ttcccgcggc gaatggcaag gtttgcaggt
    19621 taattatttc ttcaggtttc aacagaaatc cgaataccat gaaaaataac acgcgcacaa
    19681 taaaggtagc ttctaatgta atggacttaa atttatgcac ttcggcatcc agttttgcag
    19741 gattaaattt ttcgacccaa cggaactttt taatttcatc gagattgcca agaaaaaggc
    19801 caaacacaag aataaaaatc aatcccggaa ggtgataaat ttttgaaaca taatagatta
    19861 aaattatcag aatcaaaata ggcgtatagg taacatgatg tgatagtttg gccattaaaa
    19921 acgacaaaca caaaactgcc aaaaatgaga tcacaatgat caacagcatc tgcagtccaa
    19981 agtgcccgaa tgcatgccca tcgatcactt cattatacaa aataaaatta aaaaacaata
    20041 caccgataat atctgataag ctcgtttcaa aaatcacaaa ttcacgttga aaaaccgaaa
    20101 gtccgcgcac gctgggaata gctatagcac tgcttatcac acaaaatgga atagcgttta
    20161 caagtgcaac cctcaatggg agttgccaaa aaaacatgaa cattgttgta ataatagcgg
    20221 ctagcaataa aatgggtaat aatgcattaa caagcgccaa cctgatggcg gggaatttcg
    20281 aattattcag ttctaaatcg agcgcacctt ccaaaacaat aagtatcaat ccgatggtcc
    20341 ccaaaaccgg cagtatgggt tcaagatcag caacggggat atacagccat tgagttaacc
    20401 tgccaacacc aaaacctaat gccagcaata aaataacaga aggaattttt gtatgctttg
    20461 cactgatatc aattatatac gccaataaca atagtataca tactacaata actgcctgag
    20521 atgttatcat ttcgaaaaga tacaaagaaa taattttaaa atagaaataa tatttttcct
    20581 tttttaggga ataaacacaa tatccaagga gaatcatcta atgatgaatc atcttattgc
    20641 acaagttatt taattaatct cacctcactt tttcgacttt taatttatgt tattttttag
    20701 caacatcctt taaaattttg gctatccaca catcaaaacc ttaatgcagg gttgacttgc
    20761 actttattat cggcaataac acaagggcac gaatggaagc aaaacgctaa ctttgccccg
    20821 ttatgcgcgt tgaagtgtta aagagcaaga tccacagggt tagggtaacc gagtcgaatt
    20881 tgaactacat tggtagtatc actattgatg aggaccttct ggatgctgct aatatgatag
    20941 aatttgagaa ggtacaagtg ctcaatatca ataatggcgc ccgtttggag acctatatta
    21001 tcaaaggtga gcgcggttca ggtgttatat gcctgaatgg ccctgccgca caccatgtaa
    21061 cacccggcga tattgttatt gtttgttcct atgcctcaat ggaatgggag gaagccaaaa
    21121 cctggcagcc taccctgatt ttccctgaca gtcaaaccaa tatgcttcgc tgatttgagc
    21181 cgtaagctga tattcaacat ccttaaattc gcgattttcc tgggaatcgg ggtaacactg
    21241 atatggtata tcacgaaaga cctcactgcc gaccagaaac atgagctttg ggcatcattg
    21301 agtgctgcaa actattggtg ggtgggcttt tcggtgctga taggcattgc cagccatatt
    21361 gccagagcct tgcgttggaa gatgatgctc gaaccactgg gcaccaatcc tcgcctgagc
    21421 actaccttcc atgccgttat gatcgggtat cttgccaata tggcagtgcc gcgactaggt
    21481 gaaataaccc gctgcggcat cgttcaacgg tatgaaaaag tgcctttcga caaggctgtt
    21541 ggaaccctgg tggtcgagag gtctatcgac ttgctatgtc ttatcattgc cacggggata
    21601 ctattcatta cgcaattcac cctgatattc gacttctttg atgagaaggt gatatttccg
    21661 atcagccaaa aactgcaatt cagcaccgcc tttataataa tattggcagc aataatagcc
    21721 gcgattagct ttggcatctg gttttttatt aagaaatttt cgcataccga agcatacctg
    21781 cggattcgca ttctgatact caatgtgcgc gatggtattt actctatcag gcacatgaaa
    21841 aactttaaac tcttcttgtt ttataccttt ttcatctggt tttgctactt ctcctctgcc
    21901 tgggtagcct tatatgcctt aagtcagacg agcggttttg gtccgaatga ggcactaacc
    21961 atcctcgttt tcggcaccgt gggtattatt agcacgccgg gaggcatcgg cgcttatcac
    22021 ctgattgtga ctgaaactct tgtagcctta tacgggttag accgcactta tgccatcagt
    22081 ttcagctggt tggcatggag ctgtcagacc ataatgatta tctttgttgc acttatctca
    22141 ttactcgcaa tttcgcgttt gagtaagcct caacaagatg agcaagcctc tggccataca
    22201 gcataaaata atgaatctgc acacccttgt gcaacagatc agcgtatggc gattttcaaa
    22261 cagaaaaatt gtttttacca acggatgttt cgatgtatta cacaaaggac atgcacatgt
    22321 gctcaatact gccagcaacc tggcttacaa tgcagcagtt attgttggtg taaacagtga
    22381 tgcttctgta aagcgattga aaggaaatga tcgaccggta aataatgaag ccgatcgcat
    22441 gttgatgctt gcatctatgt atgcggtgga tgcggtagta ttatttcatg aggatacgcc
    22501 ttacgaactt atcaatgcaa ttcaaccgga ttttctagta aaaggtggcg attatgcgga
    22561 agaccaaatt gttggtgccg acattgttaa atcgagagga ggtaaagttg tggttgttcc
    22621 ttttttggat aattattcca ccaccctatt gattgaaaaa atgaaacact aaccaccaca
    22681 attccaaaac ctataaaacc caacaccaaa accaaaaccc atacctgtta cctgatactt
    22741 gatacttgat acctgatact tgatacctga tacttgatac ctgataccga ataaccataa
    22801 cttaaaaaat aaaacatatg tatttccaat taactgaaga acaacaaatg atccgtcagg
    22861 ctgcccgcga tttcgcacag aatgtgttga agaaaggcgt aatcgaccgc gacagagata
    22921 tgatttatcc atatgaagaa gtgaagcaaa tggcagaact
//
LOCUS       DAOQHY010000036        22740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1009_length_22740_cov_5.712806, whole genome shotgun sequence.
ACCESSION   DAOQHY010000036 DAOQHY010000000
VERSION     DAOQHY010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 22740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22740
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1009_length_22740_cov_5.712806"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(274..1707)
                     /locus_tag="PLL28_04685"
     CDS             complement(274..1707)
                     /locus_tag="PLL28_04685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNF68649.1"
                     /translation="MMRYFIATQDDFLHSWDERYHALVGKNLAKHPFEPTLKDNPILP
                     CNNLDWQSGNVWVHKQPIFLFQIAASIKVFGNSEMAVRFPSIVMGALLSLLIFRIGSI
                     TSNSLSGYFASGMFIFSFFQLEQTAGVIGMDHNDIAFAFYVTLSIWGYLEYLESGNKR
                     FVYMIALATAFGTLTKWLPALAIYGVWVIDAVLLDKQKRIHEIFLLFKSFCVSILIWG
                     SWQIYILLRWPIETKFEYEFNRRHFSEELEGHGGNWDYHIKMFDSQYGAYAFILLFVG
                     LLIFLLDKSKKRYKTLFLLIPISTYLVFSIAKTKSFAYVFYISPIVFTIIGYGLYQII
                     TLITAQVLNKVLKYVFYCAILIFYCNYQLSAKNLYKCHVLGQSEYINNYRSVLQHNAN
                     IYKQLDIVASDCDIIFNCRNMQIVDAEFYSDKTVYSWFPDSTTIDSLIDADYKIGAFQ
                     GFSNQQLPDYIKNNNKIQIIDLIQATE"
     gene            1908..3266
                     /locus_tag="PLL28_04690"
     CDS             1908..3266
                     /locus_tag="PLL28_04690"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68650.1"
                     /translation="MKLYQRFKHLIYLPCLLMMLPLSGFSQQSAWDIIKADAAPVLTT
                     TFADQFSAPEMVPLADYGWEDGLQVSYDGLHLYALYAPADLLSWVAYFAANPGLPICS
                     TLGSGAFIRDYAGDYGMDMHTNFFACDTFMNLDILYATRPSIDVPFSNWILSDIARPG
                     EIEGGPFPLFSDTDPDAVDHFLFTGPGSIWMINNTTANPSNIASAVRLPSPINPVSDE
                     FTADNPMLTRLENGNLLLIYEKYTNPDHRDFMFTISDDEGASWTTPTVITTINNDAGH
                     IEHPMLYFDGANYWFYYSQNFDIYRARQGAAGDWDSWTDHEAVILKGNTVGIGEPSIT
                     STGDIYFVAVIQNPDNPDDLMDADPWYVKNLFPIGIITNSFTAIEIHPNPADAFCTIN
                     TQVNDDYVCRITNLFGQEILSTKFIGKQMQMDVSTIPPGTYMLTLTGKKGDTWCQQMV
                     VM"
     gene            complement(3565..5718)
                     /locus_tag="PLL28_04695"
     CDS             complement(3565..5718)
                     /locus_tag="PLL28_04695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HNF68651.1"
                     /translation="MKEKLFLLSLFTVFWGCLYAQAPDIQWTKCLGGSSWDEGSACVQ
                     TPDSGVVVAGYRASFDGDCIDCPDYGYAFVVKLDSSGEIIWEKCYGGTTGEDIKSIIN
                     TTDGGLIFAGYTLSNDEDVTGNHGLSDYWVVKTDALGNLEWQKCYGGSGSDIARSIVQ
                     TIDGGYIILGRVTSEDGDVSGHHGVDSNNDYWVVKISELGEIEWEKTLGGSDEDWGES
                     IIQSQDGGFICAGYSNSDDGDIVGFHLYSDYWILKLNNEGELLWQHDFGGSSSDNAYA
                     LLEDENGDIIVSGVSYSNDGDVVGSHGASDAWVLKLNSEGNLIWNICYGGSLGDDFPS
                     VDYVSESRKILGGYSTSSNGDLSENYGSADSWIASIDSMGVLVWQKSVGGDTLDESFS
                     ISSVFDGGLVLVGYTTSNDLDASGNHGGRDIWVVKLAKPCDHQPYYADLDADSYGNPA
                     HYIYSCFDTMGYVENNLDCNDLDSLVHPFVEDICNNLDDDCDALKDEDAIALTWYLDN
                     DLDGFGNVEVDSLACFELSGYVFDANDCNDANALVNPGSIESCNEIDDNCNSEVDEGL
                     TYLHYYEDSDEDTFGNLAFDSISCAQPIGYVPDSADCNDLNPNIYPGAPEILNGLDDD
                     CDGLSDEGLSIADNTFNDVTIYPNPTNGIVTIELASEQIINVEVRTIDGKLVNTDIKV
                     VDKYHIIDLSSVAPGVYLIYIESPFWIKIVELVKN"
     gene            5889..7313
                     /locus_tag="PLL28_04700"
     CDS             5889..7313
                     /locus_tag="PLL28_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68652.1"
                     /translation="MKNPICKSTFLSMRTFKHHVYYYLVGSLFSFLFFCSFHFNLYGR
                     GNLFIETKEIISNVLSPDSTFVDTIEFTAAFSEGDTIDMYVIGADTIYTIYATPLLDT
                     SALSFRYEASPMKIILSNQTFPVEQGLLGINVTDMFEPGHANEYVDLPKYTSGENPWD
                     YLSELCPTTLRVFSGQGSRFMELLGSARTAPNDPFNTSGTMMNGGYGFCIERIIPFFD
                     NTDNDIDAPPLFDDTPGAIPFSISDDMNDDGQLNTLTSWYLSDRYKNSFEDFYHKWEE
                     QPIYDPTDPAFDEYSEQPLYINQLIHLVNKIESENPGQSVSVMLCLNILSESATNCKN
                     IATYLKDDNGIDLAGIEIGNEVYFGWAEDLLGFRDNGFVPKFIHYWDYINGFNYDDFS
                     IGVDPDDGDPGDPTGDGMYFSDFDLSLVLPSDVLLDHDYLGVLKGDPSTANIPLGLPA
                     HNLPNCGEYVFITDESDTSNTLNM"
     gene            7392..8876
                     /locus_tag="PLL28_04705"
     CDS             7392..8876
                     /locus_tag="PLL28_04705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68653.1"
                     /translation="MTSTYKFDAVILHPYYTPTNVPTDAIELCNTNWLSIPLCLDGVT
                     YGTPWTYASYDTRLKCAFDGIMGFPVIDTDADGIWDTPAVGNFRSFVTSGLKEAMDFH
                     KAHLKFSTTDVGPEMKDIWYTEYNINSDERVTDAQVEYVGSILNTFAHAEGLWNWVLW
                     NVKSNAGISYRPNYLSSTTLQSFLSGTQIALMNTASQDEQAIMGLDEISAIAPCDGGT
                     VDCSSDGDCLDNSYYLRRLTYYAMQLLNKISMDDLVYLRSTALAYVGTPVNYNIPPTV
                     FYNGNPSDPKLIVFYTNVKDVDQTFGFNPGLVYQLFPGAVSVNLYGGTINFMNANVPY
                     TTSGKCSLYNFNNWYDCQAGGSGNMYSIETDENDILNGLASTDCPVGVSGAACVVVPK
                     YSLGYFTINIDVNYRLGENKDVFAIYPNPTSSNFYIQQINSEEMVATLLDVEVYNSYG
                     ANVLSTQVVEGQRIDISNLPVGSYNVLIKSNNVLRDSEVLVKMK"
     gene            complement(9334..9828)
                     /locus_tag="PLL28_04710"
     CDS             complement(9334..9828)
                     /locus_tag="PLL28_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68654.1"
                     /translation="MEINERYIKASCGLTNGAYFEELLVDSMLEEGIPAKYRILSQFE
                     CSYQSDDEIRKEWPNKLYFKKTNDHYVWRADTLDQIRLAAETGYGRDLFKVDTLTGDT
                     IAVNMGYFVFNEASYTSCPVPFKPKTWYFLSIMQPDLLGVYLYVDEHKHFHVYEQESG
                     VSPI"
     gene            10128..11759
                     /locus_tag="PLL28_04715"
     CDS             10128..11759
                     /locus_tag="PLL28_04715"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNF68655.1"
                     /translation="MKKEQARYIFVTGGVTSSLGKGIFSASLAKLLQARGFRVTIQKF
                     DPYINVDPGTLNPYEHGECYVTEDGAETDLDLGHYERFLGVETSQANNVTTGAVYLSV
                     ITREREGEFLGKTVQVIPHITDEIQRRMLVLGENGENDIVITEIGGTVGDIESLPYIE
                     AVRQLKWKLGDSRCIVIHLTLVPYLQSAHELKTKPTQHSVKMLLESGLQADILVCRTE
                     HPLPKELRRKIALFCNVNINSVLEAADVKTIYDVPLNLLEEGADDIVLAKLKLPKKHE
                     PDLKNWKAFLSRLKNPTASVRIGLVGKYVELQDAYKSILESFIHAGAENECKVQVISI
                     QSEHITPQNVAEKLSDLDGVLVAPGFGDRGIEGKITAIQYVREHKIPFFGICLGMQCA
                     VIEFARNVLKMKDAHSTEMNPKTKHPVIDLMEEQKHVTEKGGTMRLGAYDCRLYKKTK
                     AFDIYKSQNISERHRHRYEFNNEYLKDYEAKGMMATGVNPQQNLVEIVELRDHPYFIG
                     VQFHPELKSTVENPHPLFVSFVKAAMDFKEKGPKN"
     gene            11971..13872
                     /gene="yidC"
                     /locus_tag="PLL28_04720"
     CDS             11971..13872
                     /gene="yidC"
                     /locus_tag="PLL28_04720"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNF68656.1"
                     /translation="MDRNNVIGFVLLGALIIAFFFVQSRYADNARVAEAAKQQTEDSI
                     KRVQDSLAKLELPKETAKQETTISALPDSLKNKMKPEEAKRLEDSLAAVQRANVFGSF
                     TPVMQGKEEITTLENDKLILKLSNKGGRIVQAEIKDHKNYHGGLVDVIGGDNNRFNYN
                     FYYEDNKMMNTEEMYFTPSLSSDGKTLTYTAKLSNGASLEQIYSFTNTEYLVDYNVRF
                     NGFRNVIPGRDPVIILNWRNEMQEQEKNIKYERQYSKLYFGTEDGDVDYKNTNGDLKF
                     EDRIKWVSCQQQFFNATLIAKESFEKNGKIDIFADDTSRFVKRCNTELYLTFNGKDSY
                     EFPMQWYLAPNQYDELKALDLGIETIIPTGSGFIGWINKTAIMPLFHWLSGMISNYGL
                     IILLMTLILKLALSPLTYRSYLSMAKMRVLNPEVAELREKYGDDQAKLGAEQMKLFRK
                     AGVNPLGGCIPTLLSMPILIAMFRLFPGTIQLRQQSFLWADDMSTYDDIIHWGTNIPF
                     IGTHISIFCILMTISSILYMRMNMQNTSQLTGPMKTVQYITPVFFFFFLNSSPAALTY
                     YYFVSNLTTFGQQWAIRKFLINDEEIHRRIQENKSKPEKAKSGFAKRLEDAMKQSQQI
                     REQQKKGKK"
     gene            complement(13946..15283)
                     /locus_tag="PLL28_04725"
     CDS             complement(13946..15283)
                     /locus_tag="PLL28_04725"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNF68657.1"
                     /translation="MHPTIIKNARIVNEGEILEGDVLIHKGRIEKIGGIVQVSAASTE
                     IDATGKFLMPGIIDDQVHFREPGLTHKADLFSESRACVAGGVTSFMDMPNVNPPSLTQ
                     ELLAQRYALASTKSVANYSFYMGVSNDNADEVLRTDPATVCGIKIFMGSSTGNMLVDD
                     AATLERIFSQAPVLIATHCEDEATIIHNLEAAKATYGEDIPVHMHPIIRNEEACYRSS
                     SFAVSLAKKHNTRLHILHISTAEELALFTNEIPLEQKRITAEACVHHMYFDSNDYAQL
                     GARIKWNPAVKGPRHKPAIFDAVVNDVIDVIATDHAPHTLEEKANKYVKCPGGAPMVQ
                     HSLQVMLSFYFDGKISLEKIAKKMSHDVATCFNIKDRGFIREGYFADMFLLDTDKSIV
                     VSKNNIIAKCGWSPLEGRTMRGVITDTFVNGDHVFSNGMVNDGVRGMRLAFNR"
     gene            complement(15483..16586)
                     /locus_tag="PLL28_04730"
     CDS             complement(15483..16586)
                     /locus_tag="PLL28_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68658.1"
                     /translation="MKQLALFLTGIFMAALIWVGCSTGKAGSTAQVSVPNNDSLLRYP
                     AEKHLKNIHQLTYGGDNAEGYWSFNSEMLVFQRRNMNEGVPCDQIFYGKVPTDGSRFE
                     YSLLSTGKGRTTCSYFMPGNKSVIFASTHATVDSCIADPDKSHGYLWGVYNSYEIFEA
                     DLKGNIIRQLTNNNYYDAEAVVSPKGDKILFTSDRSGDLELWTMNIDGSNLKQITNEL
                     GYDGGAFFSPDGNSIIFRASRPKTEEEVKVYKDLLAQQFVKPTAMEIFTCNADGTGLR
                     QITQLGGANWAPFFHPSGKKIIFSSNHVTKRVPFNLFMINLDGSGLEQITYDTVFDAF
                     PMFSYDGKKLVFASNRNSGGTHDTNLFVADWVD"
     gene            16669..17733
                     /locus_tag="PLL28_04735"
     CDS             16669..17733
                     /locus_tag="PLL28_04735"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF68659.1"
                     /translation="MIKGRKWLMIGSATGLLLIAILVIVLKPTVPGFGKKPDQPVSDS
                     LAMIEQLTPEIGNLSTSIHEDPKNPGLYFARANAYFDYGNLKYALADFEKAYRIDSTN
                     PAHVLGFSDCLFFLNNAEGAIAVLEDYRLHDPNDLDVLVTLAVDYYKLPKPQMQKSID
                     LLNEALKLDIQHADAYFYKGMIYKESGDTAKAISNFQTTVEVDPDFYDAYMQLGLLYA
                     AKKNSLALQYLNNALALDSTSREAHYAIAKYYQDIGKMSTAINYYKSLITDNPQDADA
                     IYNLATIYYGIDSVKAAYRYYDLAIKQSPAKAMCYYGKGLCAEELKNIKEAISLYTQA
                     LNLDPDLVLAEEKLKQLNAE"
     gene            17737..19668
                     /gene="thrS"
                     /locus_tag="PLL28_04740"
     CDS             17737..19668
                     /gene="thrS"
                     /locus_tag="PLL28_04740"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNF68660.1"
                     /translation="MIKITFPDGNVREYAEGVSSYDIAKSISEGLARKVLSAEVNGEV
                     WDLSRPINTDATFKLFTWDDQKGKSTFWHSSAHLMAEALEVLYPGVKFGIGPAIDNGF
                     YYDIDLGGKVLTQEDLPRIEEKMRELSKKNSVYQRRDVSKAEALQYFTEKGDQYKLEL
                     INDLADGTITFYTQGNFVDLCRGPHIPDTGHIKAIKLLNIAGAYWRGDEKNKQLTRIY
                     GITFPKQGELDEYLKMLEEAKKRDHRKLGRELEIYTTDESVGQGLILWMPNGAAIIEQ
                     LEKLAKRKEDQGGYLRVKTPHIAKEDLYITSGHLPYYADSMYPPMELEGTKYYLKAMN
                     CPHHHKIFDAMPRSYRELPMRLAEYGTCYRYEESGALFGLMRVRGLTMNDAHIYCTEE
                     QFESEFKAVNAMYLHYFKVFGIEKYVMRFSTHEPKKLGQKYLDNPEMWKRTEDMVRRV
                     LIDSGIPYVEVPDEAAFYGPKIDIQIFSAIGREFTLATNQVDFGQPLRFNLTYVDRDN
                     QPKVPIVIHRAPLSTHERFIGFLLEHYAGNFPVWLAPVQVAVLPISDKFNEYAEAVVN
                     VLKKHEIRTTLDTRSEKIGRKIRDAEVKKVPCMLIIGEQEMMNGTVSLRKHGEGDKGS
                     MSPEDFAKSISEEVAEVFA"
     gene            19710..20252
                     /gene="infC"
                     /locus_tag="PLL28_04745"
     CDS             19710..20252
                     /gene="infC"
                     /locus_tag="PLL28_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNF68661.1"
                     /translation="MKRVDERNYYRIGREIRHPRVRVVGENITTGVYTIQEAQRMAEE
                     LGLELVEITPNADPPVCRIVDFQKFIYEKKKKDKELKAKSKKQEMKEIRFTPHTDDHD
                     FEFKSKHAENFLVDGNKVRAYVMFRGRSIVFKEQGEMLLLKFAQRLEEVGVPDQMPKM
                     EGKRMYITFVPKAPGTKKKE"
     gene            20361..20555
                     /gene="rpmI"
                     /locus_tag="PLL28_04750"
     CDS             20361..20555
                     /gene="rpmI"
                     /locus_tag="PLL28_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNF68662.1"
                     /translation="MPKMKTNSSAKKRFKVTGSGKIKRAKAFKRHILTKKSHTRKNRL
                     GNAGLVSDADSNNIKRLLAI"
     gene            20570..20914
                     /gene="rplT"
                     /locus_tag="PLL28_04755"
     CDS             20570..20914
                     /gene="rplT"
                     /locus_tag="PLL28_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNF68663.1"
                     /translation="MPRSVPKVASRNRRKKVLKLAKGYWGRRKNVLTVAKNAVDKALG
                     YAYVGRKDKKSAYRQIWIARINAAVRPHGLTYSTFMHKLKTKNIDINRKVLADLAMNN
                     PEAFEAIVKQVA"
     gene            complement(20966..21376)
                     /locus_tag="PLL28_04760"
     CDS             complement(20966..21376)
                     /locus_tag="PLL28_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNF68664.1"
                     /translation="MVQQFNIILPAFTRGFHLIDEHILHAVGELPESGLMHVFIQHTS
                     AGLCINENADPDVRVDLETGFNKLAPEGAAYYTHTMEGDDDMPAHIKSILTGTSVSIP
                     IQHGRLAFGRWQGIFLCEFRNRGGNRRLVVTITT"
     gene            21443..>22740
                     /locus_tag="PLL28_04765"
     CDS             21443..>22740
                     /locus_tag="PLL28_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNF68665.1"
                     /translation="MASDQPQQETTTAKPRITKKSLRTAFRILAFLKPYRLAFIFGMV
                     FLLLSSFTTMAFPRLMGILIDSANPSETASWMTNTNLIGLLLGGILLVQSIFSFFRVY
                     LFATVSEKAMADVRIHFFDKLLTLDLPFYEQHRVGELTSRSTNDVDQLQDMLSATLPE
                     FVRQIVTIVFGVGFIFFISPQLTLLMISSFPILVLMAVFYGKFIRKLSKQRQDALAAS
                     NVVVEETMQNVATVKTFTNENHESKRYRTFLHRVVETGIKGAINRGGFTTFLIMGLFG
                     GFVLVIWYGTTLLKSGDIQAGDLFSFVLYTGFIGGAVGGIGDLYGRLQKTVGASERIF
                     EIIDNEGELSLDNEPDTAQRLDGKIEFRNITFSYPTRKDVTVLKDISFTINPGDKIAL
                     AGHSGAGKSTIAQLILQLYLPDAGTLLFDEQAASTFERRQ"
BASE COUNT         6992 a         4750 c         4519 g         6479 t
ORIGIN      
        1 aataatactt atcggtgctt gctgaatcga agcttgttgc tccgatccat gctgcgcctt
       61 accacatgca aaaacaccta ctaaaccaat acaagcgatg attatggctt tgcaaagaag
      121 tggggatgtg tgggattccg cggtgagcct catgatggaa tgtgtaggga ggaaatgttt
      181 cgtctgatta tttgtatttt aacaagagat taataattta atagggcatt attcttagaa
      241 atagtttaaa ggctgaatca cataaaaatg caactactca gttgcttgaa ttaaatcaat
      301 aatttgtatt ttattattat ttttgatgta gtcaggcaat tgttgatttg aaaaaccttg
      361 gaatgcgcct attttataat cagcatcaat taaactatca atagtagttg aatcaggaaa
      421 ccacgaataa actgttttgt ccgaataaaa ttccgcgtct acaatctgca tattgcgaca
      481 attaaaaata atatcacaat cagaggcaac aatatccaat tgcttgtaaa tattcgcatt
      541 gtgctgcaaa acggatcgat agttattgat gtattcagac tgacctaaga catggcattt
      601 gtacaaattt tttgctgaaa gttgatagtt acaataaaat attaatatag cacaataaaa
      661 cacatatttt aagactttat ttaaaacttg tgctgtaatc aatgtaatta tctgatataa
      721 accatagccg attatagtga acactatagg tgaaatataa aaaacatatg caaaagattt
      781 ggtttttgca attgagaata ctaaataagt agaaatcggt ataagtaaaa acagagtttt
      841 atatcttttt tttgacttat ccagaaggaa aattaacaat cctacaaata aaagtataaa
      901 agcataagcc ccatactggc tgtcaaacat tttaatatgg tagtcccaat tacctccgtg
      961 tccttctaat tcttcagaaa aatgtcgtct gttgaattca tattcaaatt ttgtctcaat
     1021 tggccatcgc aataaaatat atatctgcca actaccccaa atcagaattg aaacgcaaaa
     1081 tgacttaaat aataaaaata tttcatgaat ccttttttgt ttatctaaaa ggactgcatc
     1141 aatgacccaa acaccataaa ttgctaaagc aggcagccat ttcgtcaaag tcccaaaagc
     1201 tgtagccaaa gcaatcatat atacaaacct tttgtttcca ctttcaagat attcaaggta
     1261 gccccagata gaaagcgtaa cataaaaggc aaatgcaata tcattgtgat ccattccgat
     1321 taccccagct gtttgctcaa gctgaaaaaa agaaaatata aacatacccg atgcaaaata
     1381 gcccgacaaa gaattgcttg taatactacc aattctaaat attagcaatg acaacaaagc
     1441 tcccataaca atgcttggaa atcgaactgc catttcagaa ttgccaaaca ccttaatact
     1501 tgccgcaatt tgaaatagaa aaatgggctg tttatgtacc caaacatttc cagactgcca
     1561 atcaaggtta ttgcatggta aaattggatt atcttttaat gtgggttcaa aaggatgctt
     1621 tgccaaattt tttcccacta gagcatgata tcgctcatcc caactgtgaa gaaaatcatc
     1681 ctgagttgct ataaaatagc gcatcataaa agacgaaata aataagcagg agtaggtaac
     1741 aattacttta ctcctaaaaa aaagagcagt aaatccgaga ccataaataa taaaacttat
     1801 gtagattaaa aattcaagtt tcattcaacg caaatataaa ccctctcaag caataatttt
     1861 atattgaagg cttcatacca tccaccaaat ttccctaact tagacacatg aaattatatc
     1921 aacgctttaa gcatttgatt tatttgccat gtttactgat gatgctacca ctttcgggtt
     1981 ttagtcaaca gtctgcctgg gatattataa aagccgatgc agcacctgtc cttactacca
     2041 cttttgccga tcaattcagc gcgccggaaa tggtgcctct cgctgattat ggatgggaag
     2101 atggcttaca ggtgtcatac gacggtttgc atttgtacgc cttgtatgca ccggcagacc
     2161 tcttatcatg ggtagcttac tttgccgcaa atcccggatt gccgatctgc agcacactgg
     2221 gaagcggcgc attcatacgt gattatgccg gtgattatgg catggatatg cacaccaatt
     2281 ttttcgcctg cgatacattt atgaatctcg atattctgta tgccacaagg ccaagcattg
     2341 atgtgccttt ttccaattgg atattatcag atatagccag accgggagaa attgaaggcg
     2401 gaccattccc tttattttca gacacagatc ctgatgccgt agaccatttt ttatttaccg
     2461 gaccgggctc gatttggatg atcaataata ctacggcaaa tccttccaat attgcctccg
     2521 ccgttcgatt gccatcaccc atcaatccgg tatcagatga atttacggca gataatccca
     2581 tgctcacaag attggaaaac ggcaatttat tattgatcta cgaaaaatac accaatcccg
     2641 accatcgcga ttttatgttt accatcagcg atgatgaggg tgcctcctgg acaacaccaa
     2701 ccgtgatcac tacgatcaat aatgacgccg gacatattga gcatccgatg ttatactttg
     2761 atggtgcaaa ctattggttc tattactcac aaaattttga tatctataga gcacgacaag
     2821 gagctgcagg cgattgggac agttggacag atcacgaagc tgtgatcctc aaaggcaaca
     2881 cggtaggcat tggcgaacca tcaattacgt caacaggtga tatttatttt gtcgctgtca
     2941 tccaaaaccc ggataatcct gatgatctga tggatgcaga tccgtggtat gtaaaaaatt
     3001 tatttcccat tgggattatt acaaattcgt ttacagctat tgaaatccat cctaatccgg
     3061 ctgatgcatt ttgtacaata aacacgcaag taaatgatga ttatgtttgc cgcatcacaa
     3121 acctgttcgg ccaagaaata ctatcaacaa aatttatcgg caaacagatg caaatggatg
     3181 tttcaaccat acctccgggc acctatatgt tgaccttgac cggtaaaaaa ggggatacat
     3241 ggtgtcagca gatggtggtg atgtaactgg gttaggtgtc tccaaccata ctaatgccat
     3301 gcatctttgg cgaaagttac cataacaacc attgtaagca cttaaatcac tatagtagct
     3361 ccctcaaaac acacgataaa cagttgtgca atgtcaaagg aaaaacaaat tttcgatagt
     3421 cttagggatg gagctcaaat tcaataaatt aaactaggat agatatgtaa catcaggtaa
     3481 tatcatgctt tgcaaaaaaa tgtatttgaa tcaacaaact taatgaatat agcctatcct
     3541 cgaaattcaa aacatttgtt tgccttagtt ttttaccagc tcaactattt taatccaaaa
     3601 aggtgattca atatatatta aataaactcc gggagctacg ctagacaaat caataatgtg
     3661 atatttatcc acaaccttta tatctgtatt caccagtttt ccatcaatgg ttcgcacttc
     3721 gacatttatt atttgttctg atgccaattc gatagtaacg ataccattgg ttgggtttgg
     3781 atagattgtt acatcattga aagtgttatc tgctattgac aacccttcat cacttaaccc
     3841 atcacaatca tcatccaaac cattcaggat ttcgggtgca ccagggtaga tgttggggtt
     3901 gaggtcattg caatcggcgg agtcaggaac gtaacctatc ggttgagcgc atgagataga
     3961 atcaaaggct aaattcccaa acgtatcctc atcgctatct tcatagtaat gcaagtaagt
     4021 taacccttca tcaacttcag aattgcaatt atcatcaatt tcgttgcaag attcaattga
     4081 gccgggattg actaacgcat tagcatcgtt acaatcattt gcatcaaata catacccgga
     4141 caattcaaaa caagccaagg agtctacctc tacattacca aatccatcta aatcattatc
     4201 aagataccaa gtcagtgcaa tggcatcttc atctttcagg gcatcgcaat catcatccaa
     4261 gttgttacat atatcttcaa caaatgggtg taccaagcta tcgagatcat tgcaatctaa
     4321 attgttttca acataaccca tggtgtcaaa acagctataa atgtaatgtg ccggattacc
     4381 gtatgaatcc gcatctaaat cagcgtaata tggttggtga tcacaaggtt ttgcaagctt
     4441 gacaacccaa atatcgcgtc ctccatgatt accagaggca tctaaatcat tagatgttgt
     4501 atagcctact aacactaatc caccatcaaa aactgaactt attgaaaatg actcatccaa
     4561 agtatctccc ccaactgatt tctgccatac caagaccccc atagaatcta ttgaagcaat
     4621 ccaagaatct gctgagccat aattttctga caaatcacca ttagatgaag ttgaatatcc
     4681 tccgagaatt ttccgacttt ccgaaacata atctacactt ggaaaatcgt cacctaatga
     4741 accaccataa catatattcc aaattaaatt tccttcacta ttcagtttca acacccaggc
     4801 gtctgatgct ccgtgtgacc caacaacatc tccatcattt gaataggaaa cacctgatac
     4861 tattatatct ccattttcat cctctaacaa tgcataagca ttatcagaac tagaccctcc
     4921 aaaatcgtgc tgccataata attcgccttc attgttcaat tttaggatcc aatagtcgct
     4981 atatagatga aaaccaacta tatctccatc gtcagaattt gaatatccag cgcaaataaa
     5041 tccaccatct tgagactgaa taattgattc accccaatcc tcatctgatc cacctaaagt
     5101 tttttcccat tcaatctcgc ctaattcact aatttttact acccagtaat cattattgga
     5161 atcaacacca tgatgccctg acacatctcc atcctcacta gttaccctcc ctaaaattat
     5221 ataccctcca tcaattgtct gaacaataga tctcgctatg tcgctaccac ttcctccata
     5281 acatttttgc cattctagat ttcctaaggc atccgttttt accacccaat aatcactcaa
     5341 accgtgattc ccagtcacat cttcatcatt agaaagagta taaccagcaa aaatcagtcc
     5401 cccatcagta gtattaataa ttgactttat atcttctcct gtggttccac catagcattt
     5461 ttcccaaatt atttcaccac ttgaatcgag ttttacaaca aaggcatacc cataatctgg
     5521 gcaatcaata cagtctccat cgaatgaggc cctatagccg gctacaacta ctccactatc
     5581 aggagtttgc acacatgccg aaccctcatc ccaagaagag ccaccaaggc acttagtcca
     5641 ttgaatatca ggggcttgtg catataaaca cccccaaaac accgtaaata atgataaaag
     5701 aaatagtttt tctttcattt attgaaatta tttcaccaaa atactaaaag attttaacaa
     5761 acagtaatat aatttattaa atttggataa cacaaatatt caatgggcat aatatctatt
     5821 ccctggatat agtataaaat tccgttcttt ctattgttta attcagttaa ttccataaaa
     5881 cacaaatcat gaaaaatccc atttgtaaat ctacctttct cagtatgcgg acattcaagc
     5941 atcatgttta ttattacctt gtcggttcgc ttttttcgtt cctgtttttt tgctcatttc
     6001 attttaattt atatggccga ggtaatttat ttatagaaac caaagagata atttctaatg
     6061 ttttatcacc ggattctact tttgttgata ctattgagtt cacggctgcg ttttctgagg
     6121 gcgataccat cgacatgtat gtaattggtg cggataccat atacacaatt tatgctacgc
     6181 cgctgttgga tacatctgcc ttatcattcc gctatgaagc ctctcctatg aaaataatac
     6241 tcagtaatca aacgttccct gtagaacaag gactacttgg aataaatgtt actgatatgt
     6301 ttgagcccgg tcacgcaaat gagtatgtcg acctgccaaa atacacttcc ggtgaaaatc
     6361 cttgggacta cttaagcgag ctgtgcccga caaccttgcg tgtttttagc gggcaaggaa
     6421 gtagattcat ggagttatta ggttcagcta gaaccgcgcc aaatgatcca ttcaacacat
     6481 ccggcactat gatgaatgga ggatatggat tttgtataga acgtataatt cctttttttg
     6541 ataacacgga taatgatata gatgctccgc ccctttttga tgatacacct ggggcaattc
     6601 catttagtat ctcagacgac atgaacgatg atggtcaact taatacgctt acatcatggt
     6661 atctttcaga ccgatataaa aattcctttg aagatttcta ccataagtgg gaagaacaac
     6721 cgatatacga tccaaccgat cccgcctttg atgaatattc ggaacaacca ttgtatatta
     6781 atcagttgat tcacttagtc aacaaaatag aatcagagaa ccctggccag agtgtgagcg
     6841 taatgctatg tcttaatatt ttaagtgaat cagctactaa ctgcaaaaat attgccacct
     6901 atctgaaaga cgataatggc atagacttgg ccggaattga gattggaaat gaagtgtatt
     6961 ttggatgggc tgaagacctt ttaggcttta gggataacgg atttgttccc aaattcattc
     7021 actattggga ttatataaat ggttttaact atgatgattt ttctatcgga gtggatcccg
     7081 atgatggcga tccgggcgac ccaaccggtg atggtatgta ctttagtgat ttcgatttat
     7141 cacttgtatt gccaagtgat gtattattgg accatgatta tttaggcgta ttgaaaggcg
     7201 acccttccac agctaatatc ccacttggat tgcctgctca taatttgcca aactgtgggg
     7261 aatatgtatt tattaccgat gaaagcgata cttcgaacac cctaaatatg tgaggtgcag
     7321 atccttgtgc tgactgcaca tatcccttgt ggaatgacca attagctgca aaatattcag
     7381 cacagattcc aatgacctct acctataaat ttgacgctgt gattctgcat ccatattaca
     7441 caccaacaaa tgtgcctaca gacgctattg aactgtgtaa taccaattgg ctcagcattc
     7501 ctttatgcct tgacggtgta acttatggca caccgtggac atacgcttca tatgacacca
     7561 ggcttaagtg cgcctttgat ggcataatgg gatttcctgt aatagacaca gatgctgatg
     7621 gcatatggga tacacctgca gttggaaatt ttagaagctt tgttacctcc ggcttaaaag
     7681 aggctatgga ttttcacaag gcacacctta aattcagcac tactgatgtt gggcctgaaa
     7741 tgaaggacat ttggtataca gagtacaata ttaatagtga tgaaagggtt actgatgcgc
     7801 aagttgaata tgttggatca attctaaata cctttgccca tgctgaaggc ttgtggaatt
     7861 gggttctatg gaatgttaaa tcaaatgctg gcattagcta tagaccaaat tacctttcaa
     7921 gcactacctt gcaatcattt ttgagcggca cgcaaattgc attaatgaac acagcaagtc
     7981 aagatgaaca agcaataatg ggtctagatg aaataagtgc catagctcct tgcgatggcg
     8041 gtactgttga ttgttccagt gatggagatt gccttgataa ttcatattat ttgaggcggt
     8101 tgacatacta tgcaatgcaa ttgttgaata agataagtat ggacgattta gtatatctga
     8161 gaagcacagc attagcttat gtaggcacgc ctgtcaatta caatatcccg ccaactgtct
     8221 tttataatgg aaatccatca gaccctaagc ttattgtttt ttatactaat gttaaggatg
     8281 ttgatcaaac atttggcttc aatccaggat tagtttatca gctattccca ggggctgtat
     8341 ctgtaaattt gtatggaggt acaattaatt ttatgaatgc aaatgtccca tatacaactt
     8401 cagggaaatg ttccctatac aatttcaata attggtacga ctgccaagct gggggtagtg
     8461 gcaatatgta ttcaattgaa acagatgaaa atgatatatt gaacgggctc gctagtaccg
     8521 attgtcctgt cggagtttcc ggtgcggctt gtgtcgtagt tccaaaatac tcactcggct
     8581 acttcacaat taacattgat gtaaactatc gactagggga aaacaaggat gttttcgcca
     8641 tttatcccaa ccccacctca agcaattttt acatccaaca aattaattca gaggaaatgg
     8701 tagcaactct attagacgtt gaggtatata acagttatgg tgctaatgta ttatctacgc
     8761 aagtggttga aggtcagcgt atagatattt ccaaccttcc tgttggcagc tacaatgttt
     8821 tgattaaatc aaataatgtt ttacgagata gcgaagttct agtgaaaatg aagtaactaa
     8881 aatcatttcc aaccacgcca taagaattaa tttaaatata gcttagtttt cacaggtatt
     8941 gccaagggct cgaatttcat tcggggcctt ggctttttcc aatctaatta tgtcattaca
     9001 aacaaaacct aatcaaaatt tactaacata aaatcagaga gaaaaccgac aacaaatagt
     9061 tcatggactt gacctacatg cgattaaagt gaggtgtaat attttaaata gaataggtta
     9121 gcgttgcaac ctcatattag cctttgagtt ggcattcact ttctttcaat atcaagctaa
     9181 ttcaatttgc aattaaatta cctagcgttg taaaattaca aacaagaata caactgccat
     9241 tttttttgaa tttttaaact acttggcata tattacatcg aagcaaaaga tccatcaggc
     9301 aactctgcaa tagaaaaatt agtcattttc tgatcatatt ggagaaacgc cactttcctg
     9361 ttcataaaca tggaaatgct tgtgctcatc aacatacaaa tagacgccta atagatcggg
     9421 ctgcatgatg cttagaaaat accatgtctt tggtttgaat ggaaccgggc atgatgtata
     9481 ggatgcttca ttgaaaacaa aatatcccat atttacggct atggtatcac ccgtcagggt
     9541 atcaacttta aacagatctc gaccataacc tgtttctgca gccagcctga tttgatccaa
     9601 tgtatcagca cgccaaacat agtgatcatt tgtttttttg aaatacaatt tattcggcca
     9661 ctctttccgt atttcatcat cagattgata ggagcattca aattgagaaa gtatcctata
     9721 ctttgccgga attccctctt ccagcatact gtcaacaagc aattcctcga aataggctcc
     9781 attagtgagt ccgcaggagg ctttgatata ccgttcgttg atctccacat tgcaaggtct
     9841 gaacacatat gatagcagca atccacaacc tgagaacaaa aaacctgcgc acacaggtat
     9901 ggcgatcgat cgggttattc ggaggaggct catccaatga aggtaaggat ccattccttt
     9961 cgttgggagg gatggcagaa tgttctcaac atcttaccct catcattttt ccgcacaggc
    10021 gtaggatttc agaggaaagc ggcctgtaaa acgccgttgc aatgtgttaa actgcaccct
    10081 cgaagagttt gaacattgtg attttcaccc tacctttaaa gcccgttatg aagaaagaac
    10141 aagctcgcta tatcttcgtt acgggaggcg ttacatcttc gctcggtaag gggatctttt
    10201 cagcatcatt agctaaactt ttacaagcac gcggttttcg tgtgaccatt caaaaattcg
    10261 atccatacat taacgtggat ccgggtacct tgaatccgta tgagcacggc gaatgttacg
    10321 ttaccgaaga tggtgccgaa actgaccttg accttggcca ttacgaacga ttcctgggag
    10381 tggaaacctc gcaggccaac aacgtgacta caggtgccgt ttatctgagt gtgatcaccc
    10441 gagagcgcga gggagaattt ttaggtaaga ctgtgcaggt tattccacat atcaccgacg
    10501 aaatacaacg ccgtatgctt gtgttgggtg aaaatggcga aaatgatatc gtgatcactg
    10561 agatcggcgg tactgtgggt gatattgagt ctttaccata tatcgaagca gttcgtcagt
    10621 tgaaatggaa actgggcgac agccgttgta tcgtaataca tctcacgctt gttccctatt
    10681 tgcaatcggc acatgaacta aagactaaac ccacgcagca ctctgtaaag atgttgctcg
    10741 aaagtggttt gcaggccgat attcttgtat gcagaaccga gcatccattg ccaaaagagt
    10801 tgcgcaggaa aatcgcactt ttttgtaatg taaatatcaa ttcagttctc gaagcagccg
    10861 atgtaaagac tatttatgat gtgccattga atttgctgga agaaggtgcc gatgatattg
    10921 tacttgcaaa attaaaattg ccgaaaaaac acgaaccgga cctgaagaat tggaaggcat
    10981 tcctatcgcg attgaagaat cctacagcat cggtgcgcat cggtcttgtt ggtaaatacg
    11041 ttgaattaca ggatgcctat aaatccattc tggaatcctt tattcatgca ggtgctgaaa
    11101 atgaatgtaa ggtacaggtg atcagtattc aaagcgagca tatcaccccg cagaatgtcg
    11161 ccgaaaaact cagcgacctt gatggtgtgc tggtggctcc gggcttcggc gatcgcggta
    11221 ttgaaggtaa gatcacggca atacaatatg tgcgtgagca taaaattcca ttcttcggca
    11281 tttgtctcgg catgcaatgt gcagtaattg aatttgcacg caatgtgttg aagatgaaag
    11341 atgcgcacag cacagaaatg aatcccaaaa caaaacatcc tgttatcgac ctgatggaag
    11401 aacagaaaca tgttaccgaa aaaggcggca ccatgcgttt gggagcatat gattgcaggt
    11461 tgtataaaaa gactaaagct tttgatatct ataaatcaca aaacatcagc gagcgacatc
    11521 gccatcgcta tgaattcaac aatgaatatt taaaagatta tgaagcaaaa ggtatgatgg
    11581 caacaggtgt gaatccgcaa caaaatcttg tagagattgt tgagttgcgc gaccatcctt
    11641 atttcatcgg tgttcagttc catccggaat taaaatcaac cgtagaaaat ccgcatccgt
    11701 tgtttgtttc atttgtgaag gcggcaatgg atttcaagga aaaaggccct aaaaactaag
    11761 tcattcgctg ataaccaggc ttccggcata acatcaggtc tcaaggtcat tacggaactt
    11821 ccagcttgaa aattgagctt ttaacaatgt ttttatgcgc tgatcgtcat cacttaaaac
    11881 caaggcagaa taggtatttc agggcttccg ggctttatat aggctacatc agcgatttga
    11941 tgtaactttg ccgctcaatt taacagtata atggatcgta ataatgtcat cgggtttgta
    12001 ttgctgggag cgctcattat agcgttcttc ttcgtacaaa gccgctatgc agataatgct
    12061 cgtgtagcag aagcagccaa gcaacaaacc gaagattcca tcaaacgggt gcaggactcg
    12121 ctggcgaaat tggaattacc caaggaaacc gccaaacagg aaacaaccat ttctgcactt
    12181 cccgatagcc tgaaaaacaa aatgaaaccc gaagaggcaa aacgtctcga ggattcattg
    12241 gcagcagtgc aacgcgcaaa tgtatttggc tcttttacac ctgtaatgca gggaaaagaa
    12301 gaaattacca cacttgaaaa tgataagctc atacttaaat tatctaacaa aggtggtcgc
    12361 attgttcagg ctgaaataaa ggatcataaa aattatcacg gcggattggt agatgtaatt
    12421 ggtggcgaca ataatcgctt taactataac ttttattatg aagacaataa gatgatgaat
    12481 acggaagaga tgtatttcac tccttccctt tcatcagatg gcaaaacact tacctatacc
    12541 gccaaactaa gcaatggcgc atctttagag cagatttaca gcttcacaaa taccgaatat
    12601 ctggttgatt acaatgtgcg tttcaacgga ttccgcaatg taattccagg tcgcgaccct
    12661 gttatcattc tcaattggag aaatgaaatg caggagcagg aaaaaaatat caaatacgag
    12721 cgtcagtatt ctaaattata tttcggaaca gaagatggtg atgttgacta caaaaacacc
    12781 aatggcgatc tcaaatttga agatcgcatt aaatgggtga gctgtcagca acaatttttt
    12841 aatgccactc ttattgcaaa agagagcttt gagaaaaatg gcaagatcga catttttgcc
    12901 gatgatactt ctcgatttgt gaagcgttgt aataccgaat tatacctcac atttaatggc
    12961 aaagattcct atgagtttcc catgcaatgg tatcttgccc caaatcaata cgacgagtta
    13021 aaagcacttg atctcggtat tgaaactatt attccaacgg gttcaggatt tatcggttgg
    13081 attaacaaaa cagctattat gcctttgttc cattggctga gcggtatgat cagcaactat
    13141 ggactgatca ttttgttgat gaccttgatt ctgaaactgg cattgtcgcc gcttacctac
    13201 cgctcttatt tatctatggc gaagatgcgt gtgctcaatc ctgaagttgc cgaattacgc
    13261 gaaaaatatg gtgatgatca ggctaaatta ggtgctgaac aaatgaaatt gttccgcaaa
    13321 gcaggtgtga atccattagg aggttgtatc cctaccctgc tgtcgatgcc aattctcatc
    13381 gcgatgttcc gcttattccc cggaacaata caattgcgtc aacaatcatt cttgtgggct
    13441 gatgacatgt cgacttacga tgatatcatt cattggggca ctaatattcc attcattgga
    13501 acacatatca gtattttctg tattctgatg actatcagca gcattctgta catgcgcatg
    13561 aatatgcaga atacttctca acttacaggc ccgatgaaaa cggtgcaata tatcacacca
    13621 gttttctttt tcttcttcct gaatagttca cctgcagcct taacatatta ttacttcgta
    13681 agtaacctta ccactttcgg acagcaatgg gcaatcagaa aattcctgat caatgatgaa
    13741 gaaattcatc gtcgcattca ggaaaacaaa tcaaaacctg aaaaagcaaa atccggattt
    13801 gcaaaacgtc tcgaagatgc catgaaacaa tcgcaacaaa ttcgtgagca acagaaaaaa
    13861 ggtaaaaagt aattactaca ttaacataaa aaatggcgat ttgaaattat caagtcgcca
    13921 ttttaatttt ggtttatcgt catcctcatc tattaaaagc caatcgcatg ccgcgaacac
    13981 cgtcgttcac cataccattg ctgaatacat gatcgccatt tacgaatgta tctgttatca
    14041 caccgcgcat agtacgtccc tccagcgggc tccagccgca tttggcaatg atattatttt
    14101 tcgatacaac aatgctttta tcagtatcaa gtaaaaacat atcagcaaaa tatccttcgc
    14161 gaataaatcc tctgtcctta atattaaaac aggtggcaac atcgtgactc attttcttcg
    14221 cgattttttc cagagaaatt ttcccatcga aataaaaact caacatcacc tgcaaactat
    14281 gttgcaccat gggcgctcct cccgggcatt tcacatactt atttgctttc tcttcaaggg
    14341 tgtggggtgc atggtctgtg gcaatcacat cgatgacatc attcactacg gcatcaaaaa
    14401 ttgcaggctt atgtcttgga ccttttaccg caggattcca cttgatgcgt gctcccaatt
    14461 gcgcataatc attgctgtcg aaatacatat gatgcacaca tgcttcagcg gtaattcttt
    14521 tctgttcaag agggatttcg ttggtaaata aggcaagttc ttctgcagta ctgatatgca
    14581 atatatgtaa tctcgtattg tgttttttag caagtgaaac agcaaatgaa gatgaacgat
    14641 aacaggcttc ttcattgcgg ataatcggat gcatatgcac cggaatatct tctccataag
    14701 tagctttggc agcttccaaa ttgtgtataa tggttgcttc gtcttcacaa tgtgttgcga
    14761 ttaacacagg agcctgagaa aaaatgcgct ctagtgtagc agcgtcatca accaacatgt
    14821 tacctgttga agaacccatg aagattttta ttccgcaaac agttgccgga tcggttctca
    14881 atacttcatc ggcattgtcg ttgctcacac ccatgtaaaa tgaataattg gcaacacttt
    14941 ttgtcgatgc caacgcgtag cgctgtgcaa gcaattcctg tgtcaaactc ggaggattca
    15001 cattgggcat atccataaat gaggttacac cacccgcaac acaagctctg ctttcggaaa
    15061 agagatcagc tttatgcgtg agtccgggct cgcgaaaatg cacttggtcg tcaataatac
    15121 cgggcatcag gaatttccct gttgcatcta tttcggttga tgcagcactg acttgtacaa
    15181 taccaccgat tttttctatt ctgcctttat gtatgagaac atcgccttca agaatttctc
    15241 cttcattgac gatgcgtgca ttttttatga ttgttggatg catatttgat tgcaacacgt
    15301 ttcatgctgt tgcctgttca caaatatacc atgtaatcag gtaattggtg gtacgtgttt
    15361 gtggtgtgtg tttgtttcac gcagaggcgc agagacgcag agtgagggtg cgggaaataa
    15421 taaagtaaag gcgccatcga aagaaatttt cgatgagttg gataaggctt atatgtttta
    15481 tatcaatcga cccaatcagc tacaaacaaa ttggtgtcat gtgtgccgcc gctgtttctg
    15541 ttgcttgcga atacaagttt ttttccatcg tagctaaaca tcgggaaggc atcaaacacg
    15601 gtatcgtaag tgatttgctc tagtccggag ccatcgaggt tgatcataaa taaattaaaa
    15661 ggcacgcgtt tggtaacatg gttgctgctg aaaataattt ttttgcctga tggatgaaag
    15721 aaaggtgccc agtttgctcc gccaagttgc gtaatctgac gcaagcctgt tccatcggca
    15781 ttgcatgtga atatttccat cgccgtaggc ttaacaaact gctgtgcaag caggtctttg
    15841 tatactttaa cttcttcttc agttttcggg cgacttgctc tgaaaataat gctattcccg
    15901 tctggtgaaa agaatgcgcc gccgtcgtat cccaattcat tcgttatttg tttgagattg
    15961 gagccatcaa tattcatggt ccataattca aggtcgccac tgcggtcgct ggtaaacaat
    16021 attttatcgc cttttggact tacaaccgct tcggcatcat aataattatt gttcgtgagc
    16081 tgacgaatga tattcccttt caggtcagct tcaaatattt cgtaactatt ataaacgccc
    16141 cagaggtagc cgtgactttt atccggatcg gcgatacagg aatctacagt ggcatgcgta
    16201 gatgcaaaga tcacgctttt gtttcccggc ataaaatagc tgcatgttgt gcggcctttt
    16261 cccgtgctga gcaaactgta ttcgaatcgt gaaccatcgg taggaacctt gccataaaag
    16321 atttgatcgc aaggaacacc ctcattcatg tttctgcgtt gaaacactaa catttcgctg
    16381 ttaaaactcc aatatccttc cgcgttatcg cctccgtatg taagctgatg gatgtttttt
    16441 aagtgctttt cagccggata acgaagtagt gaatcattgt taggcacgct tacctgtgcc
    16501 gtagatcctg ccttacctgt gctgcaacct acccaaatga gggcagccat gaaaattcct
    16561 gttaaaaata gagcgagttg tttcattatg cgctgaaaat tgcggcaaag ttacgatgta
    16621 gacatcattt cccctgatat aacaacgtta ataccgtaat tttgcgatat gattaaaggt
    16681 cgaaagtggt taatgattgg aagtgcgaca ggtttactcc tgatcgcaat tttggtcatt
    16741 gtcctaaaac caactgttcc cggttttgga aaaaagcctg atcagcctgt cagtgattct
    16801 ttggcaatga tcgagcaatt gacaccggag atcggaaatc tttcgaccag tattcacgaa
    16861 gaccctaaaa atccggggct gtatttcgct cgtgcaaatg cttactttga ttatggcaat
    16921 ttgaaatatg ctcttgccga ttttgaaaaa gcttatcgca tagattctac aaaccctgcg
    16981 catgtcctgg gcttcagcga ttgcctcttc tttttgaaca atgccgaagg agccattgcc
    17041 gtgctggaag attatcgcct ccacgacccg aatgatttag atgtactagt cacacttgca
    17101 gtcgattatt acaaacttcc aaagcctcaa atgcaaaaat caatcgattt gttgaacgag
    17161 gcgcttaagc ttgatattca gcatgccgat gcttattttt ataagggtat gatttacaaa
    17221 gaaagtggag ataccgctaa agcgatttcc aattttcaga ccactgttga agtagatccc
    17281 gatttttatg atgcctatat gcaactcgga ttgctatacg cagccaagaa aaacagcctg
    17341 gcgctgcaat acctgaacaa tgcgctggca ctggattcta ccagtcgaga agcacattat
    17401 gccatagcaa aatactatca ggacatcgga aaaatgtcta ctgccatcaa ttattataaa
    17461 tctttaataa ctgataatcc gcaagacgcc gatgccattt acaacctggc aaccatctac
    17521 tatggcatcg atagcgtgaa agctgcctat cgttattatg acctggccat aaagcaatca
    17581 ccggcaaaag ccatgtgtta ttatggcaaa gggttatgtg ccgaagaatt aaaaaatatc
    17641 aaggaagcta tttccttata tacgcaggct ttaaatttag acccggatct ggtattagcc
    17701 gaagagaaac tcaaacaatt aaatgcagaa taattcatga ttaagatcac ctttcccgat
    17761 ggtaatgtgc gggagtatgc agaaggggtg tcgagttacg acatcgccaa gtcgattagc
    17821 gaaggacttg cgcgcaaagt attgagcgcg gaagtgaatg gagaagtatg ggacctcagt
    17881 cgccccataa acacagatgc caccttcaag ctgttcacgt gggatgacca gaaagggaaa
    17941 tctacatttt ggcatagcag cgctcacctc atggctgagg cattggaagt gctatatccc
    18001 ggtgtgaagt ttggaattgg tcctgccatt gataatggat tttattacga tatcgatctg
    18061 ggcggaaaag tgcttacgca ggaagacttg cctcgcattg aagaaaagat gcgcgagtta
    18121 tcgaaaaaga attcggtgta ccagcgtcgc gatgtttcta aagccgaagc attgcaatat
    18181 ttcaccgaaa aaggcgatca atataaatta gagctgatca atgatctggc cgatggcact
    18241 attaccttct acacccaagg gaattttgta gacctgtgtc gcggaccgca tattccggat
    18301 accggccata tcaaagcaat caagttgttg aacattgccg gtgcttattg gcgcggcgat
    18361 gaaaagaata aacagcttac acgcatctat ggtatcactt ttcctaagca aggtgagctc
    18421 gatgagtatt taaaaatgct cgaagaagcc aaaaaacgcg accatcgaaa actcggccgt
    18481 gaattggaga tttatactac cgacgagtcc gtaggtcaag gccttatttt gtggatgccg
    18541 aacggtgctg ccattattga acaattggaa aaactggcaa agcgcaaaga agatcagggc
    18601 ggctatttaa gggtgaagac accgcatatt gcgaaagaag atttgtacat caccagtggt
    18661 cacttgccat attatgcaga tagcatgtat ccgccaatgg aattggaagg aacaaaatac
    18721 tatctcaagg caatgaattg tccgcatcac cataagatat ttgatgccat gccacgcagt
    18781 tatcgcgaat tgccgatgcg actggcagaa tacggcacct gttatcgcta tgaagaaagt
    18841 ggcgcacttt ttggattgat gcgcgtgcga ggactaacca tgaacgatgc gcatatctat
    18901 tgcacagaag agcagtttga aagcgagttc aaggcggtaa acgctatgta tttgcattac
    18961 ttcaaagtgt ttggcattga aaaatatgtg atgcgttttt cgacacatga acctaaaaaa
    19021 ctggggcaga aatacctcga caatcccgaa atgtggaaac gcaccgagga tatggttcgc
    19081 agggtattga tcgacagcgg tattccgtat gtggaagttc cggatgaagc agcgttttat
    19141 ggtccgaaaa ttgatattca aatattctcg gcaataggac gtgaatttac actggcaacc
    19201 aatcaggtag atttcggtca gccgttgcga tttaatttga cttatgttga ccgcgataat
    19261 cagccgaaag tgcctatcgt gattcatagg gcaccattga gcacccatga gcgattcata
    19321 ggatttttgc tggagcacta tgccggtaat ttcccggtat ggctggcgcc ggtgcaggtg
    19381 gctgtgttgc ccatcagcga caagttcaac gagtatgccg aggcagtggt aaatgtgtta
    19441 aaaaaacacg aaattcgcac caccctggac acaaggagcg aaaaaattgg tcgcaagatc
    19501 cgtgatgcgg aggtgaaaaa agtgccttgc atgctgatca tcggcgaaca ggaaatgatg
    19561 aatggcactg tatcgcttcg caagcatggc gaaggagata aaggcagcat gagtcctgaa
    19621 gatttcgcga agagtatcag tgaagaagta gcagaagttt ttgcataaat tattgttaac
    19681 caagtaatta tataaccaaa attaaaagtt tgaagcgagt agacgaacgc aattattatc
    19741 ggatcgggcg agagatccgt caccctagag tacgtgtagt gggtgaaaac attaccaccg
    19801 gtgtttatac cattcaggaa gctcagcgca tggccgagga acttggtttg gagctggtag
    19861 agatcactcc aaatgccgat cctccggtat gccgcattgt cgattttcag aaattcatct
    19921 atgaaaagaa gaaaaaagac aaagaattaa aggccaaaag caagaagcag gagatgaagg
    19981 agatccgctt caccccacat accgacgacc acgatttcga gttcaagagc aagcatgccg
    20041 agaactttct ggttgatggg aacaaggtaa gggcctatgt aatgttcagg ggccgttcca
    20101 tcgtattcaa ggagcaggga gaaatgcttt tgttaaagtt tgcccagcgc ctggaagagg
    20161 ttggtgtacc tgatcagatg cctaaaatgg agggtaaaag gatgtatatc acctttgtac
    20221 ctaaggctcc cggaaccaaa aagaaagaat aactaatctt ttcacggcaa agaagttgca
    20281 tttgggcttt gaagtttcag cccgaactcc tatctttgcg ctcccgaact ttcggggaag
    20341 taaacaaaac aagtgtcatt atgccgaaga tgaagaccaa cagcagcgcg aaaaagcgct
    20401 tcaaggtgac cggtagcgga aaaatcaagc gcgcgaaagc gttcaaacgc catatcctca
    20461 ccaaaaagag tcataccagg aaaaaccgcc tcggtaatgc cggtctcgta agtgatgcgg
    20521 attcaaacaa cattaaacgt ttactagcca tctaataaac aagtagatta tgccaaggtc
    20581 agtacccaaa gtcgcgtcgc gcaatcgtag aaaaaaagta ttaaagcttg ccaaaggcta
    20641 ttggggccgt cgcaagaacg tattaactgt tgccaaaaac gcagttgata aagccctcgg
    20701 ttatgcatat gtaggtcgta aagacaagaa atctgcttac cgccaaattt ggatcgcgcg
    20761 catcaatgct gctgttcgcc ctcacggact tacttacagc actttcatgc acaagctcaa
    20821 aacaaaaaat atcgatatca accgcaaggt tttagctgat ttagctatga acaatcctga
    20881 agcgtttgaa gcaatcgtaa aacaggtagc ataagcatcc tgctataaaa gataaagatc
    20941 cctccgcatg ccggggggat tttttttatg tagtaatagt aactaccaac cggcgattcc
    21001 ctcccctatt gcgaaattca cacaggaaaa tcccctgcca cctgccaaat gccaatcgcc
    21061 catgttgaat gggtatgctc acactcgtac ctgttaaaat gcttttaatg tgcgcaggca
    21121 tgtcatcgtc gccttccatc gtgtgggtat aataagcagc accctcagga gctagtttgt
    21181 tgaatccggt ctcgagatct acacgcacat cgggatcggc attttcattg atgcaaaggc
    21241 ccgctgaagt gtgttgaatg aatacatgca tcaaccctga ctcaggcaat tcgcctactg
    21301 catgcagaat atgctcgtcg atgaggtgga agccgcgtgt aaaagccggc agtatgatgt
    21361 taaattgctg caccatcaat caaaaataaa atgaaaacgg taactactcg tcaactatcg
    21421 gggaatatct ttacaccttc ttatggcatc tgatcaaccg caacaggaaa cgacaacagc
    21481 aaaacccagg atcaccaaaa aaagtctccg cacagccttc agaattcttg ctttcctgaa
    21541 gccttatcgc cttgccttta tttttggaat ggtgttttta ttgctctcga gttttaccac
    21601 catggcattt ccacgcttga tgggaatact catcgactca gcaaatccaa gcgagactgc
    21661 gtcctggatg acaaacacca atttaattgg cctgttgctg ggaggcatac tcctcgttca
    21721 atcgatattt tcattcttca gagtatacct tttcgccact gtttctgaaa aagcaatggc
    21781 cgatgtgcgc atacattttt tcgataaact gcttaccctt gatctccctt tttacgagca
    21841 acaccgggtt ggcgaactaa ccagcaggtc aacaaatgat gtggatcaat tgcaggatat
    21901 gcttagtgct accttacccg aatttgttcg acaaatagta acgatagtgt ttggtgtggg
    21961 atttatcttt ttcatctcac cgcaacttac actgctcatg atatcatcat ttccaatttt
    22021 ggttttgatg gcagtgttct acggaaaatt catccgcaaa ctttccaaac aaaggcagga
    22081 tgctttggca gcttcaaatg tggttgtgga agaaaccatg cagaatgttg ctacggtaaa
    22141 aaccttcacc aatgaaaatc atgaatcaaa acgctatcgc acatttttac atcgcgtagt
    22201 ggaaacaggt attaaaggcg ctattaatcg cggcggattt accactttcc tcatcatggg
    22261 attatttggc ggatttgtat tggtaatctg gtatggcaca acgctgttaa aatcgggtga
    22321 tattcaagcc ggcgacttat tttcatttgt gttatatacc ggtttcattg gaggtgctgt
    22381 tgggggcatt ggcgatctgt acggccgact gcaaaagact gttggcgcat ctgaacgcat
    22441 tttcgagatc attgataatg aaggtgaatt atctctcgat aatgaaccgg ataccgctca
    22501 acgtcttgat ggtaaaattg aattcagaaa tattacgttt tcatatccta cgcgaaaaga
    22561 tgtaaccgta ttaaaagata tttcattcac cattaatccc ggcgataaaa ttgcattggc
    22621 aggtcacagt ggcgccggaa aatctaccat cgcgcaacta atattacaac tatatcttcc
    22681 cgatgcaggc acgctgttat ttgatgaaca ggctgcatca acattcgagc gccgacaatt
//
LOCUS       DAOQHY010000037        22699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1012_length_22699_cov_4.886195, whole genome shotgun sequence.
ACCESSION   DAOQHY010000037 DAOQHY010000000
VERSION     DAOQHY010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 22699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22699
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1012_length_22699_cov_4.886195"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="PLL28_04770"
     CDS             <1..1085
                     /locus_tag="PLL28_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNF68666.1"
                     /translation="NWDGNLSREQVVRAMALHKPSFEFRDTYGYCNAGFVAAAEVLYA
                     VTDTTIDDFLKAHIFTPLQMTRTSTTHAAIAADKNACKPYTVFEGRLVQLEYDNVDNL
                     AAAASINSSAHDLANWLIMQLNNGKFNDVQVLPKQVVNNPRLPRTVVGSGNSSIYKSQ
                     HFNLYGLGWFIKDFEGRRVYSHDGGANGFVTGTTFIPEAGLGIVVLTNTDANAFYDAL
                     RYQIMEAYFNVPYRNISDIYLARVTPGNEAQDSVLHSYQAIAEKNLTPALPIASYAGK
                     YHNEVYGDMEIKESNGTLVMTFSHHPQLTGSLRASGGNNFICFFDPVSWGVKEFPFTV
                     VDGKVKSVTVTINDFIDFMPYEFVKQ"
     gene            1087..1662
                     /locus_tag="PLL28_04775"
     CDS             1087..1662
                     /locus_tag="PLL28_04775"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HNF68667.1"
                     /translation="MQEIYAAVIMPPEEIAETIRIFKDGLAGVVGKYSSSASDPVIHL
                     FSAVGDNERISQLEEKLEVLCRNQLPFNVHLDHFDEFHSHKTVFVDISETSKKAITSL
                     RKKLSSELKSSKYDEEVDFSVGKTPHITIARNLPDDQYRVARNYFTGRTYDAYFECSS
                     ITWRKLIRSNNNTYYETVREFPFAMKNLSLF"
     assembly_gap    2043..2142
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2271..2370
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2371..3881)
                     /locus_tag="PLL28_04780"
     CDS             complement(<2371..3881)
                     /locus_tag="PLL28_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNF68668.1"
                     /translation="MQEIIEVVISPEKVADDQAIFKTLEDVCGFAPAHWKIRKRSLDA
                     RGRSPVYRMQIIVSSAPIVELAYQRPAFKSIKDAAPVIIIGAGPAGLFAALTCIKQGI
                     KPVIFERGKDVRKRRHDLAALNRDGIVNPDSNYCFGEGGAGTYSDGKLYTRSDKRGNI
                     REVLETLVQFGADENILVDAHPHIGTNRLPKIIENIRNYILDAGGEIHFESKVSELMI
                     TDNVIKGIKLANGETHHAHAVILATGHSARDIFFMLHKQQIQIEAKPFALGVRIEHPQ
                     SLIDSMQYHCADRGPYLPPSPYSFVEQVNGRGVYSFCMCPGGIIAPCATAPGEVVTNG
                     WSPSKRNNPFANSGFVVGIELKDMREFEQYGALAGLYFQMAVEKAAYKAGGGKQKAPA
                     QRMVDFVDKKLSATLPDCSYHPGITSAPLEEVLPVFVANSLRQAFVQIRKKRPAYFTN
                     EAVCVATESRTSSPVRIPRDPQSLQHPQISGLYPCGEGAGYAGGIVSAAMDGIR"
     gene            complement(3915..>4424)
                     /locus_tag="PLL28_04785"
     CDS             complement(3915..>4424)
                     /locus_tag="PLL28_04785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68669.1"
                     /translation="SDIPGYVYNETDTGITGLQTEWRDAYIFIIFYMTASPNGYELPD
                     EDTVRLRIQLQTDSIDNKTAGIAIDEFSYKFHIWGNVQDYHATTSLHISPNPVSANCQ
                     LFDASLKNTIQRLDIYNMTGQLMQTFPNVVFDASGRAVLSLEALPSGTYICDIASDQS
                     NYRGVVVKL"
     assembly_gap    4425..4524
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4770..5900)
                     /locus_tag="PLL28_04790"
     CDS             complement(4770..5900)
                     /locus_tag="PLL28_04790"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HNF68670.1"
                     /translation="MEKVHERLQRELQTREEQHALRKLSVTDDLIDLASNDYLGFAKD
                     AQLRAMIDEEVKSLPFVGSGGSRLLTGNNAYVELVEKEICEFHQSDAALFFNSGYEAN
                     SGLISCVVHRHDIILYDELIHASLREGIKLSNAKAYAFLHNDIHDLKAKLEMHKGNVF
                     IITESVFSMDGDLCPLIEIIEIAEQFQAAIILDEAHATGVIGERGEGLAQYLGVQDSI
                     FARVHTFGKAIGANGAVILGSLILRDYLINFCKPFIYTTAPNYLQLAAVRMAYRFLRE
                     DTTPLQHLRANLELMKSLLEMSNFRKSVSTNSAIFSFVVPGNDRVKSLAAAMRSNGFD
                     MRPILSPTVPAGMERIRICVHAFNTHPEIEGAIRILASNMPN"
     gene            complement(5915..6889)
                     /gene="bioB"
                     /locus_tag="PLL28_04795"
     CDS             complement(5915..6889)
                     /gene="bioB"
                     /locus_tag="PLL28_04795"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HNF68671.1"
                     /translation="MGDIRNNWTKEEISEIYYLPMMELIYRASTMHRKYHKTGEVQVC
                     TLLSIKTGGCAEDCAYCPQAARYHTDVEVHKLLEYDEVITKALIAKEFGSTRFCMGAA
                     WRNVRDNKDFDHVLQMVKGVADMGMEVCCTLGMLTEEQAVKLKDAGLYAYNHNLDTSE
                     EYYEAIITTRTYDDRLETIENVNKAEISVCSGGIIGLGESEDDRIGMLHTLSTLPKHP
                     ESVPVNALVPVKGTPLEHNKRVSVWEMVRMIATARIVMPKAMVRLSAGRNEMSVEEQA
                     LCFMAGANSIFAGEKLLTTPNPDADEDKAMFQLLGLSPRPAYKEEPVS"
     gene            6937..7359
                     /locus_tag="PLL28_04800"
     CDS             6937..7359
                     /locus_tag="PLL28_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68672.1"
                     /translation="MAFMHQFNYKHLIGLLLAGAVVLGTGELKDKKADKAPSEEVQAL
                     DAKLLKDLQHQEIQIDVKTATLMECGDVTESELRKLFDINNVNYNKCESGNCHYTSYT
                     IEGKLDNGTEVLFKLDSGEDGNVLRDLTFQGETIPCAM"
     gene            complement(7329..8210)
                     /locus_tag="PLL28_04805"
     CDS             complement(7329..8210)
                     /locus_tag="PLL28_04805"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNF68673.1"
                     /translation="MRSVIHWILYSNLLIAVAAAAYTLNGSMQMDLSQNIDPVLTWFV
                     GSSTLFIYLLIRYAAVSRIQEYTESDRWNFFLKYLHVFKVILIISGILSFVLYLFLPR
                     STELILLFPGGISMLYGLPIRFGKKKIRLRDIGIIKIFLISFVWAFVSSILPAVYVDV
                     NWRSPEVLWIFLANYIYIFAITLPFDIKDMEIDAMHHVRTIPVLIGKTMTISLSMLLL
                     FVSAMIYDLYYTVWFIRDISLEVPHIISILITGMTILLANNIKRDTIYFGLLDGMIIL
                     QFLLVGYSYMAQGIVSP"
     gene            8255..9421
                     /locus_tag="PLL28_04810"
     CDS             8255..9421
                     /locus_tag="PLL28_04810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNF68674.1"
                     /translation="MEVILQRGKARRVEDGHPWVYGNEIDVITGEPQTGDIVDVFNFK
                     KDFIGRGYINTKSQITVRILTRQRSEPIDISFFRKRLFACREYREKLGFTLNYRLVFG
                     EADFLPALVIDKFDDVYVMQTLSAGMDKWKRVIADILLDDFGASGVYERNDVPVRTLE
                     GLEETTGFLSNEFDTRFVIDEDGVKFYVDLVNGQKTGFFLDQKENRLALQHIVKNAEV
                     LDCFTYTGSFALYAAKFGARHVTALDISAEAIRLMQENVALNNFTNVEGVCANAFDVL
                     PVWVKENRLYDVVILDPPAFTKNRKGIENAVKGYKEINLRGIRMVRPGGFLATFSCSH
                     FMDVNLFYDTVASAAKDAGRTVREVQFLNQSKDHPVVWGIQETHYLKGLILQII"
     gene            9439..10284
                     /locus_tag="PLL28_04815"
     CDS             9439..10284
                     /locus_tag="PLL28_04815"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNF68675.1"
                     /translation="MKNIYTFLALCILSSTAFAQTPSCCAAVGQFANFSHDIAFVKTH
                     EDPIPFQLTGQLGKMQTFACKDSSFATVYTVQAEQPSNKYLLVFHEWWGLNDYIKQTS
                     DKLYTDLGKNVHVIAVDLYDGKVAANKDSAASYMQTLTYDRAAMIINSLMDKFGDDVE
                     IATIGWCMGGGYSLQASLMAKERAVGCVMYYGFPETDKSKIANLSSDVLMIWPNQDKW
                     ITKEVVDKFKSDMQAAGKNLQVEEYTADHAFANPSNPKHDPVITADANAKALLFIKTH
                     LKIAD"
     gene            complement(10358..10888)
                     /locus_tag="PLL28_04820"
     CDS             complement(10358..10888)
                     /locus_tag="PLL28_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNF68676.1"
                     /translation="MEQIIIATGIGAFGGWAYWRWIGCSSGTCAITKNKYMSILYGAL
                     LGLMVVTSGGCKSSNDTDVSKTGTTIQTASIFENISPAAFADKLQEPGVMLIDVRTPE
                     EFAEGHLAGAINMNINDSGFESSLARLDKSKTYLIYCRSGARSGRAGNLMASQGFTTI
                     YNLDGGILAWKGEVVK"
     gene            complement(10891..11196)
                     /gene="trxA"
                     /locus_tag="PLL28_04825"
     CDS             complement(10891..11196)
                     /gene="trxA"
                     /locus_tag="PLL28_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNF68677.1"
                     /translation="MSTTSAFSELINGDQPVLVDFFAEWCGPCKTMAPILRDFAGEKS
                     GKVRVIKVDIDKNPGVAGQYQIQGVPTLILFKAGKPVWRQSGVVPLSYLHQAVDKVI"
     gene            complement(11251..11847)
                     /locus_tag="PLL28_04830"
     CDS             complement(11251..11847)
                     /locus_tag="PLL28_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNF68678.1"
                     /translation="MTEHLSKEYWEQRYADGTTGWDMGVVAPALKHYFDSIERKDAAI
                     LIPGCGNAWEAKYLVEQGFTNITVIDIAEPPVNHLREALGADAQQQCNVVLGDFFAHA
                     GKYDYIIEQTFFCAIDPALRPAYVKHMAQLLKPKGELAGLLFKTPFDKPGPPYGGTEA
                     EYREVFAGSLNIIEMIPCEHSHPARAGNELFFRAGLLS"
     gene            complement(11844..12488)
                     /locus_tag="PLL28_04835"
     CDS             complement(11844..12488)
                     /locus_tag="PLL28_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNF68679.1"
                     /translation="MDDIRALLDNHFPQFEDALVDRIASVATLRTFARDEVMMYSGRY
                     FKSTILIVGGRVKLYRESNDGKEYFMYYIEPGNACALSMICAARNQASEITAVAVEDT
                     QVIQVPLEIMDDLMREYKSWYYFVLETYRSRFEELLVVIDQIAFKHMDERLEHYLFNQ
                     FNEYNSRRLEITHQQIANDLSSTREVISRLLKKMEQNGKIKLHRNEIELINLFV"
     gene            complement(12517..12753)
                     /locus_tag="PLL28_04840"
     CDS             complement(12517..12753)
                     /locus_tag="PLL28_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68680.1"
                     /translation="MWAKRFAAGWQYYLMASLTLGLAPFTPEPHIWGKIRWIAGGAVG
                     MQAIDWFDVFLHGTPWLLLIAALLIKAFRTGKQE"
     gene            complement(12743..13057)
                     /locus_tag="PLL28_04845"
     CDS             complement(12743..13057)
                     /locus_tag="PLL28_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNF68681.1"
                     /translation="MIDLLKKMLGGGPKVDYAQLVKDGAVIIDVRTVGEFNGGHAKGA
                     INIPLDKLEGSIKKIKNKGPIIVCCASGMRSGSAKSILKSHGFTEVYNAGSWYNLRSH
                     VG"
     gene            13338..15005
                     /locus_tag="PLL28_04850"
     CDS             13338..15005
                     /locus_tag="PLL28_04850"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68682.1"
                     /translation="MKTKFSFETYTLLASAFLGINRSGAQVVIKDYDPDLRMVKWDDT
                     FNLHDTVKIDIDENGINDLSFIYDYSGLDVIIEIEIADMVTGFGAEHDFSYDLWALDV
                     YAFHEGDIVDESVHFKDDGQNVASAWVTFTESGYYWNVYYRGNFNSYGYYLPLELNID
                     DSIHYGWARLSIGPLGNDLYNDMWYTTDYSLVVYEVGYEASPNTPIVCQTESPIEDIN
                     PILKNETDGDDFSEFTYFYHSAYFTDYAELRIFLIDNYNDAKNFSVAQAKLIDPANYE
                     VISGDTVPSYTDIYKHFDADTRTINGDPFDPNKYYVAYFMKIPLDGDTTHLTLSTPSK
                     LIKASKNACTLSLESSITKVNTTGTAADFDVTFSKDADESDIQEYRVGLISDPTEVFD
                     LANIVLDNYTLSVPITGASEYTLNLDGINYELNGSLIFFDQYYHAVIAAVSDGYLCDI
                     SCYVVTPNNAKTLIPNTAQSPGVTLTNYANLLTIQIDQPLQSEQAHLVLSSIAGEKVI
                     DTELVAAITDFNLNELPTGIYFATIYCKGQPIASKKIIVSLTTKGSH"
     gene            15404..16627
                     /locus_tag="PLL28_04855"
     CDS             15404..16627
                     /locus_tag="PLL28_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68683.1"
                     /translation="MKRGSLLIAIPFLGISAYGQTIDSAKVQSVLDKLELDVYGEIFY
                     NAYQWETFPKKNNDIDLSRLVLYPEYHFNDQIGVEMEIEFEHGGTGATMEYDYIEEYG
                     EFEMEIEKGGEVSIEELELNYTPTPWMEIIAGRIGLPIGLIAGDDKPLNYFTTTYNNV
                     EATLIPTAWYEFGAGAEFNFRNWEAKLVVVNALNSSLFSSANWIKPGNAMRFETTAAD
                     AFAVCLGINYNLNENSHIGVSTYRGNSTPNRPKEDISVDGNVSIADAHAKLQFGRWKI
                     NGLLIYGHLQNADLISEANRNLSNNLNAKRTPVASETMGYLGEIAFNILPKTNEHYRA
                     LDIFAGYYYYDSMYGTTGDIFNNPRWEKTEWRGGFCFTWNDNIAIKSDVTYRTLNIPD
                     VNTEITYTTALAFQF"
     gene            16638..17702
                     /locus_tag="PLL28_04860"
     CDS             16638..17702
                     /locus_tag="PLL28_04860"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HNF68684.1"
                     /translation="MFNIQLKHLLTLPATAIIISSCNNNDDPSDIGTYDFSPILNHVS
                     ENVITATYGDLHSKSQSLITAVENFNADRSSTNLEAARQAWRDCRIPWEQSEGFLFGP
                     VSTEGIDPAIDSWPVNVIDLDAVLASGESLTKAYVDALEGTLKGFHTIEYLLWGTDGD
                     KSAAAFTDREFEYLSAVTQSLEGATQQLYDAWHSGGDNFQLNIAQAGTATSIYPSQKS
                     AMQELVNGMIGIADEVANGKINEPLTNMDVTLEESHFSSNSKADFMDNIRSIKNVYLG
                     SYLMDGEGISDYIKSLNSSVDDDVIAHIDAAILAIENIPGDFSDAIFMNTASVEAAQQ
                     SVRDLQSILESAVKPLIDNY"
     gene            17727..19094
                     /locus_tag="PLL28_04865"
     CDS             17727..19094
                     /locus_tag="PLL28_04865"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HNF68685.1"
                     /translation="MRRSLVVMSIITSGIIFLTIHACSIDPQVYGEWYNEEEYYAGGE
                     TTIFEATSGAFSTPAPNLSPLQLEKHLSGDMEFEATFVTAPSPVNAGLGPVFNQTSCV
                     GCHTLDGRGDKPTVFRISTPGTDAHGGPLGVAGFGGAIQDKAIFDVAPEAMVDISYVE
                     STYSFPDGETYSLRKPIYTIINNYAPLPSDVALSVRVAPPVFGLGLLEIIPEEAIIAL
                     ADEQDADKNGISGKANYVYDVRTGLMALGRFGWKANQPNLDQQNAAAFNEDMGITNPI
                     FQTESCFGQLQFDGITDEPEITMPVLEDVTFYVRTLAVPAPRNLEDANIQEGKMYFYK
                     AGCNTCHNPSFTTGIAINAPELSNQKIYPYTDMLLHDMGDAMGDGRQDYLATGNEWKT
                     RPLWGIGLTQLANGHTVFLHDGRARNLTEAIMWHGGEAEASTEYFASLTKEQRDKLLE
                     FLMAL"
     gene            complement(19995..20363)
                     /gene="ssrA"
                     /locus_tag="PLL28_04870"
     tmRNA           complement(19995..20363)
                     /gene="ssrA"
                     /locus_tag="PLL28_04870"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            20434..22128
                     /locus_tag="PLL28_04875"
     CDS             20434..22128
                     /locus_tag="PLL28_04875"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNF68686.1"
                     /translation="MASDNTNNATQRPYPRIIPDIDNWPIYTLSKDREQLIESVIDET
                     LENILKKNPTEKDIHDELAKAVYLEKIRVERPWKTDAPDEKTFWNGVKNKLVQLESHP
                     DNKIDEEKAILRTIIHRYVHEIVGNFNKGTYNFAERFVPFAFNRLLNASHSRAFTRLF
                     GNKRDLLKSIHLIGEIEQVRELAKDGIIVAVPTHSSNIDSITVGWGISAIGLPAMFYG
                     AGLNLFSVRFLAYFMNRLGAYRVDRRKKNAVYLEALKTYSTQVITKGGHSLFFPGGTR
                     SRSNVVESQLKLGLLGTALEAQRRLILETNGQSFKKVYVVPVTMNYGFVLEAKELIDE
                     HLKNVGKERYFVPSDKSSSSFKILKFMYRFFTADADFTIAFGKCMDIFGNPVDKQGNS
                     LDHNGNMIDIRDYFRSHGQLTNDTQRDSVYTKMLSEAIVVAFKKNNVALPEHVIAFAA
                     FKLFQKKHHTSDLYSVLRMAEEFRELTREELKQAVASIVKELSKLEHKEEIRVDPIVA
                     AEDLDLLIDYAIKKLGVYHQKRPLLKTRKGTIKCQDMKLLLFYHNRLEGYGLEKHV"
     gene            22121..>22699
                     /locus_tag="PLL28_04880"
     CDS             22121..>22699
                     /locus_tag="PLL28_04880"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNF68687.1"
                     /translation="MSDKTSVGVVGSGSFGTAVANLLAENSHVFLYSRRADNVVSMAN
                     ARISSGQQLHPNITPTDDIGLIAKECYVIFPIVSSDGFKEVIRTMAPFLRPDHILIHG
                     TKGLALKKKGLEKLKEKSRISNRNVLTMSQLIRRESVVVRVGSMAGPNLAREIAQEMP
                     AATVIASRFDEVIHEGHRLLTSPRFQVYGSYDI"
BASE COUNT         6692 a         4783 c         4619 g         6305 t
ORIGIN      
        1 taaactggga tggcaattta agtcgcgaac aagtggtgcg agcaatggca ttacacaaac
       61 cttcttttga atttcgcgat acttatggat attgcaatgc aggatttgtt gctgctgctg
      121 aagtattata tgccgttaca gatactacaa ttgacgattt cctgaaagca catattttca
      181 ctccgttgca gatgacgcga acaagcacaa cgcatgctgc tatcgctgct gataaaaatg
      241 catgcaaacc atataccgtt ttcgagggtc gacttgtaca attagaatac gacaacgtag
      301 acaatctcgc agcagcagct tccataaatt cgtctgctca tgatttggca aattggctga
      361 tcatgcaatt aaacaacgga aagtttaacg acgtgcaagt gctgcccaaa caggtagtga
      421 acaatccgcg cttgcctcga acagtagtag gttcaggaaa ttcaagtatt tataaatcac
      481 agcattttaa tttatacgga ctgggctggt ttatcaaaga ttttgagggg cgacgcgtgt
      541 atagccacga tggtggcgca aacgggtttg taaccggaac aacatttatt ccggaagccg
      601 gtttgggtat tgttgtgctt acaaataccg atgccaatgc cttttacgac gccttgcgtt
      661 atcagattat ggaagcatat ttcaatgttc cttatcgcaa tatttctgat atctatctgg
      721 caagggttac accgggaaat gaagcgcagg attcagtgtt acacagctat caggccattg
      781 cagaaaaaaa tctcacacct gcattaccaa tcgcatccta cgccggaaaa tatcacaacg
      841 aagtgtacgg cgatatggaa ataaaagaat caaatggaac actggtaatg acattcagtc
      901 accacccgca attaacaggt tctttgcgtg catcaggcgg aaataatttc atttgctttt
      961 tcgaccctgt gtcctggggc gtgaaagaat ttccttttac tgttgttgac gggaaggtaa
     1021 aatcggtgac ggttactatt aatgacttta ttgatttcat gccatatgaa tttgtaaaac
     1081 aataacatgc aggagatcta tgcggcagta atcatgccac ccgaagaaat tgctgaaacc
     1141 attcgcattt ttaaggacgg attggcgggt gtagtcggca aatacagcag cagtgcttcc
     1201 gatccggtga tacatttatt ttctgcagta ggcgataatg agcgtatcag ccaacttgaa
     1261 gaaaaattag aagtgctgtg ccgaaatcaa ttgccgttta atgtgcacct ggatcacttc
     1321 gacgagtttc attcgcataa aacggtgttt gttgatattt ctgaaacatc gaaaaaggca
     1381 atcacttcat tgagaaaaaa actttcttca gaactcaaat cttcaaagta tgatgaggaa
     1441 gttgatttca gcgtgggaaa aactccgcat atcactatcg cacgcaatct ccccgacgat
     1501 caatatcgcg tggcaagaaa ttattttaca ggcagaactt atgatgctta tttcgaatgc
     1561 agctccatca catggcggaa actaatacga agtaacaata atacctatta tgaaaccgtt
     1621 cgcgagtttc catttgcgat gaaaaatttg tctttatttt agttgtgtgc tttttcgcgc
     1681 aaaggcctgc cggccggcaa ggcgggcgca aaggagctaa ggcgcaaaag tttggtggta
     1741 aggtgcaagt ggcatttcac gattcacgat tctcccttgt ttaatcgcgg agtttgcgga
     1801 gttagcggag tcagagaatc actcacgagt cacggctcac gagtcaccct tgtttaatcg
     1861 cggagttagc ggagtcagag ggtcagtcac gagtcacgac tcacgattca cgattcaccc
     1921 ttgtttaatc gcggagtttg cggagttagc ggagtcagag ggtcagtcac gattcacgac
     1981 tcacgagtca cccttgttta atcgcggagt ttgcggagtt agcggagtca gagggtcagt
     2041 cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgattcac gacaatatat
     2161 ctatatccga agcaaaacca ccattcaatt tcaacccttc atccgctcta caatacgaat
     2221 accatccata gcagcgctca cgattccccc tgcataaccg gctccctctc nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgaataccat ccatagcagc gctcacgatt
     2401 ccccctgcat aaccggctcc ctctccgcat ggatataacc cactaatttg cggatgctgc
     2461 agggattgtg gatctcgggg aatgcgcaca ggtgatgagg tgcgtgattc tgtagctacg
     2521 caaacggctt cattggtaaa ataggccggt cgctttttcc gaatttgaac aaatgcttgt
     2581 cgcaggctat tggcaacaaa taccggcaac acctcttcaa gtggcgcaga agtaattccg
     2641 ggatgatagg aacaatcggg taaagtagca gacaactttt tatcgacaaa atctaccatg
     2701 cgttgagcgg gtgctttttg tttgccgcct cctgctttat atgctgcttt ttctactgcc
     2761 atctgaaaat ataatcctgc taaggcacca tattgctcaa attcgcgcat gtcctttaat
     2821 tctattccca caacaaatcc actattggca aagggattat tgcgtttgct cggactccag
     2881 ccattggtga ccacttcacc gggagcagtt gcacatggtg cgataatacc accggggcac
     2941 atacaaaaag aatatacacc tcgaccgttt acctgctcaa caaaactgta tggcgaaggc
     3001 gggagatatg gtccgcgatc agcacagtga tattgcatgc tatcgataag tgattgcgga
     3061 tgttcaatgc gcacacctaa tgcaaatggt tttgcctcaa tctgaatctg ttgcttatgc
     3121 aacataaaga aaatatcgcg cgccgaatgt cctgtggcaa gtatcacggc atgtgcatga
     3181 tgcgtttctc catttgcaag ttttataccc ttaatgacat tatcggtaat catcaattct
     3241 gacaccttgg attcaaaatg tatttctcct ccggcatcaa gaatataatt tctgatattt
     3301 tcaataatct ttggaagtct gttcgtgcca atatgcggat gtgcgtcgac aagaatattt
     3361 tcatctgcac caaattgcac taatgtttct aatacttctc tgatattccc gcgtttatcg
     3421 cttcgcgtgt ataatttgcc atcgctgtaa gtgccggcac caccttcacc gaagcaataa
     3481 ttactatcag gatttacaat gccatcgcga ttgagcgcag caagatcatg gcgacgtttg
     3541 cgcacatctt ttccgcgttc gaaaataacg ggtttgatgc cttgtttgat acaggtcaaa
     3601 gctgcgaaaa gtcccgcagg tccggcgccg atgatgatta ccggagcagc atccttaata
     3661 gatttaaatg cagggcgctg atatgctaat tctacaattg gcgcgctgct tacgataatt
     3721 tgcatacggt aaaccggtga tcgacctctg gcatcgagag atcgcttacg aattttccaa
     3781 tgtgcaggag caaacccgca gacatcttcc aaggttttaa aaatagcctg atcgtctgct
     3841 actttttcgg gggagataac aacttcaata atttcctgca tgaaatataa gtcccatcaa
     3901 ttgggactac aaatctataa cttcacaacg acacctctgt aattcgactg gtcagaagcg
     3961 atatcacata tatatgttcc cgatggcaag gcttccaaag aaagcaccgc tcttcccgat
     4021 gcatcaaata caacattggg gaatgtttgc attaattgcc cggtcatatt ataaatatca
     4081 agcctttgta tggtattctt gagagatgca tcaaatagtt ggcaattagc agaaacagga
     4141 tttggtgaaa tatgtaatga tgtggtagca tgataatctt gtacatttcc ccaaatatga
     4201 aatttataag aaaattcatc aattgctatt ccggccgtct tgttgtcaat tgagtcagtt
     4261 tgcaattgaa tgcgtaatcg cacggtatcc tcatcaggca actcataacc attgggcgac
     4321 gcagtcatat aaaatataat gaaaatatat gcgtctctcc attcagtttg aagacctgtt
     4381 atacccgtat cagtttcgtt atatacataa ccgggaatat ctgannnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnntatttc atccgttact aagcaattgg gagaattcca
     4561 acaattgttc aatgttttct tatccggatt accaatttcc caaatagagg tatcgttgat
     4621 aggttttaaa taaggacaag tttcttcgaa gtcacactcg tcttcccaaa ggaattgggc
     4681 cgacaaacaa tacgtctgaa tacaaatagt taatagagca agtaattttc tcatggcggg
     4741 gaatgctctt caaatatact aaaagatcat caatttggca tattcgatgc taatatcctg
     4801 attgcacctt caatttcggg atgcgtatta aaggcatgca cacaaattct tatcctttcc
     4861 atacctgctg gcacggtggg tgacaagatg ggacgcatat cgaagccatt ggagcgcatg
     4921 gctgctgcca gtgatttgac acggtcgttg ccgggaacca cgaaactgaa gatggcagaa
     4981 ttggttgaaa cagacttcct aaaattcgac atttccagca aacttttcat caattccaaa
     5041 tttgctctca aatgttgcaa tggcgttgta tcttccctca gaaagcgata tgccatgcgc
     5101 actgcagcca attgcaggta attcggcgca gtggtataga tgaatggttt acaaaaattg
     5161 atgaggtaat cacgcaaaat cagacttcct aaaataacgg ctccatttgc accaattgcc
     5221 ttaccaaaag tgtgtacacg agcaaagatg ctatcctgaa caccaagata ttgtgccagc
     5281 ccttcccctc tttcaccaat cacacctgta gcatgtgctt catccaatat gatggctgcc
     5341 tgaaactgct ctgctatttc aatgatttca atcagcggac ataaatcacc atccatgctg
     5401 aacacagact ctgtaatgat gaaaacattc cctttgtgca tttccagttt cgccttgagg
     5461 tcatgtatat cgttatgcag gaatgcatat gctttagcat tgctaagctt aattccttct
     5521 ctgagcgatg catgtatcag ctcatcataa agaatgatgt catggcggtg taccacacac
     5581 gaaattaatc cgctgtttgc ctcgtaacca ctgttgaaaa atagtgctgc atccgactga
     5641 tgaaattcgc agatttcttt ttcaaccaac tctacatagg cattatttcc ggtaagcaat
     5701 cgagatccgc cgctgccaac aaatggcaat gatttaactt cttcatcaat cattgctctt
     5761 aactgagcat ccttcgcaaa tccgagataa tcgttagatg ccaggtctat taaatcatct
     5821 gttacagata atttgcgcag tgcatgttgt tcttcacgcg tttgaagttc tcgctgtaaa
     5881 cgttcatgca ctttttccat cattttccaa ttaatcaact tacaggctct tccttatatg
     5941 caggtcgagg tgaaagaccc agcaattgaa acatcgcctt atcttcatca gcatcaggat
     6001 taggcgtagt cagtaatttc tcaccggcga aaatactatt cgctccggcc ataaaacata
     6061 atgcctgttc ttcaacgctc atttcatttc ttcctgcact cagtcgcacc attgctttag
     6121 gcattacaat acgagctgtt gcaatcatgc gcaccatttc ccaaacggaa acgcgtttat
     6181 tatgctccaa tggtgttcct ttcactggaa ccaaggcatt taccggaacc gattcaggat
     6241 gttttggcaa tgtagaaagt gtatgcaaca tacctatgcg atcatcctcc gattcaccaa
     6301 gaccgatgat gccgccgctg cacacagaga tttctgcttt gtttacattt tctatcgtct
     6361 ccaaacgatc atcgtaagta cgagtagtaa tgatcgcctc ataatattct tcactggtat
     6421 ccagattgtg attataagca tataatccgg catcttttaa tttcaccgct tgctcctctg
     6481 tgagcattcc caaagtacag caaacttcca ttcccatatc cgctacacct ttcaccattt
     6541 gcaatacgtg gtcgaaatct ttattgtcgc gaacattgcg ccaagccgcc cccatgcaaa
     6601 atctggtgga accaaattct tttgcaatta gcgccttggt aatcacctcg tcatattcca
     6661 acagtttatg tacctccaca tcggtgtgat agcgtgctgc ttgcggacag taagcgcaat
     6721 cctctgcaca tccacctgtt ttgatactca acaaagtaca aacctgcact tcaccggtct
     6781 tgtgatattt acgatgcatg gttgatgcac ggtatatcaa ttccatcatc ggcaaataat
     6841 atatttccga aatctcctct tttgtccagt tgttacggat atctcccatg agctgctttt
     6901 ggagcaaaat tcgtaatttt gaggcatatt tccgttatgg ctttcatgca tcaatttaat
     6961 tataagcacc tgataggact gttactggct ggtgctgtgg tattgggaac cggcgaattg
     7021 aaagataaaa aggccgacaa agccccatca gaagaggttc aggcgctcga tgccaaattg
     7081 ttaaaagacc ttcaacatca agaaatacag atagatgtta aaactgcaac attgatggaa
     7141 tgtggcgatg taacagaaag cgagttacgt aagctttttg acatcaataa cgtcaattac
     7201 aataaatgcg aatcaggaaa ttgtcattat acttcgtata cgattgaagg caaacttgac
     7261 aatgggactg aagtgctgtt caaactggat agtggtgagg atggcaatgt gctgcgcgat
     7321 ttaacatttc agggagaaac aattccctgt gccatgtaac tatatcctac cagcaaaaac
     7381 tgaaggatga tcataccatc caataatccg aaataaatcg tatcgcgttt aatattgttc
     7441 gccaataaaa tggtcatgcc ggtaattagg atactgatga tatgtggcac ttccaaagaa
     7501 atatcacgga tgaaccacac agtgtagtaa agatcatata tcatcgctga gacaaacaac
     7561 aataacatcg ataaactgat agtcattgtt tttccaatca acaccggaat ggtgcgcaca
     7621 tgatgcattg cgtcaatttc catgtccttt atatcgaaag gaagtgtgat tgcaaaaatg
     7681 tatatataat tagctaagaa tatccacaac acctcagggc tgcgccaatt tacatcaaca
     7741 tacacagcag gtaatattga agatacaaaa gcccaaacaa atgatatcag aaatattttg
     7801 atgatgccaa tatcgcgcaa acgtattttc tttttgccaa agcgaatggg caatccatat
     7861 aacatagata tgcctcccgg gaacaacaaa atgagctcag tcgatcttgg caagaacaaa
     7921 tacaatacaa aacttaatat ccccgatatt attaatatca ccttaaacac atggagatac
     7981 ttcaagaaga aattccatct gtcgctctca gtatattctt gaattctgct aacagctgca
     8041 tatctgatca gcaaatagat aaacaaggtt gaactgccga caaaccatgt taaaacaggg
     8101 tcaatatttt gcgacaaatc catttgcata ctgccgttca gcgtataagc cgccgcagca
     8161 acagcgatca gcaaattcga atacaatatc caatgaatga cggatctcat aagcagccgt
     8221 tgtaatttcg taatttcacc caaatttaca gaaagtggag gttattcttc agagaggaaa
     8281 agcacgtcgc gttgaagacg gtcacccttg ggtgtatggc aacgagatcg acgtgatcac
     8341 cggtgagcct caaaccggcg atattgttga tgtattcaat ttcaagaaag attttattgg
     8401 tcgcggatat attaacacga aatcgcaaat taccgtgcgc attttaacac gtcaacgcag
     8461 tgagccgatc gatatctcct ttttcagaaa acgcctcttc gcttgtcgcg aatacagaga
     8521 aaaattagga ttcacactga attatcgttt ggtattcgga gaagctgatt ttctgcccgc
     8581 attagtgata gataaattcg atgatgtata tgtcatgcag accttaagtg caggcatgga
     8641 taagtggaag cgtgtaatcg ccgatattct gcttgatgat tttggagcat caggtgttta
     8701 tgagcgaaat gatgttcctg taaggacact tgaaggattg gaagaaacaa caggatttct
     8761 ttcaaatgaa tttgatacgc gttttgtcat tgatgaagac ggtgtaaaat tttatgttga
     8821 tttagtgaat ggccaaaaga caggtttctt tttagaccag aaagaaaacc gtcttgcttt
     8881 gcaacacatc gtaaaaaatg ccgaagtgct cgattgtttt acctatacag gttcatttgc
     8941 actctatgca gcaaaattcg gtgccaggca tgtaactgcg cttgatattt ctgcggaagc
     9001 gattaggttg atgcaagaga atgttgcact gaataatttc acgaatgttg aaggtgtctg
     9061 tgccaatgca tttgatgtat tgccggtatg ggtgaaggag aacaggctat atgatgtagt
     9121 aattctcgat ccccctgcat ttaccaaaaa ccgaaaaggc atagaaaatg cggtaaaggg
     9181 atacaaggaa atcaatcttc gcggcattcg catggtacgt cctggtggat ttctggccac
     9241 cttcagttgc tcgcatttca tggatgtgaa tttattctat gatacagttg catcagccgc
     9301 taaagatgca ggccgaactg tgcgtgaagt acaatttttg aaccaaagta aagatcaccc
     9361 tgttgtatgg ggaatacagg aaacacacta tctcaaagga ttaattttac aaattatata
     9421 atatcaaatt tataaggcat gaaaaatatc tacacctttc ttgcattgtg catactctcg
     9481 tcaactgcat tcgcacaaac cccaagctgt tgtgccgccg ttggtcaatt tgcaaatttt
     9541 tcacatgata ttgcttttgt aaaaacccat gaggatccga ttccattcca actgaccggg
     9601 cagcttggca agatgcaaac atttgcttgc aaagacagca gtttcgcaac cgtttatacc
     9661 gttcaagctg aacagccatc aaataaatac ttactcgtat ttcatgagtg gtggggatta
     9721 aatgattaca tcaaacaaac ttcagacaaa ttatatactg atctgggaaa aaatgtgcac
     9781 gtaattgccg tggacttgta cgacggcaaa gtggctgcaa ataaagattc ggctgcaagt
     9841 tatatgcaaa cactcactta tgatcgcgct gctatgatca tcaattcgct gatggataaa
     9901 tttggtgatg acgttgaaat tgccactata ggatggtgta tgggcggcgg atatagtttg
     9961 caggcttcat taatggcaaa ggaacgcgct gtaggttgtg tcatgtatta tggatttcct
    10021 gaaacggata aatcaaaaat cgccaattta tcatccgatg tattgatgat atggccgaac
    10081 caggataaat ggattactaa agaagtggtc gataagttta aatcagatat gcaagcggca
    10141 ggaaaaaatt tgcaagtgga agaatatacc gccgatcatg cttttgccaa tccaagtaac
    10201 cccaaacacg atccggttat tactgctgat gcaaatgcga aagcactatt gtttatcaaa
    10261 acacatttaa aaattgccga ctaaatcata tacaaagtca aaggccgctc cttatcggat
    10321 gcggcctttt aactaccctg ttgtatatgc tctttaatta tttgactact tctcccttcc
    10381 aggcaagtat accgccatcg agattgtata tagtcgtaaa accttgtgat gccattaagt
    10441 ttcctgccct tccgctacgt gcgccgctcc tgcaataaat aaggtaagtt ttagatttat
    10501 ctaaacgcgc taatgatgat tcaaatccgg aatcattgat attcatattt attgctcctg
    10561 ccaaatgacc ttcagcaaac tcctcaggtg tgcgcacatc aattagcata actcccggct
    10621 cttgtaattt atcggcaaag gctgcaggag aaatattttc aaaaatggat gccgtttgga
    10681 tggttgttcc cgtcttagat acatccgtat cgtttgacga tttacatccc cctgatgtaa
    10741 ctaccatcaa acctaataaa gcgccataaa gtatgctcat atatttattt tttgtaatcg
    10801 cacaggttcc tgaactacat ccgatccacc gccagtaagc ccacccgcca aatgctccaa
    10861 ttccagttgc tatgatgatc tgttccatga ttaaataact ttatccaccg cctgatgcaa
    10921 atagctcaat ggtacaaccc ccgactgtcg ccataccggt ttacctgctt taaaaagtat
    10981 taaagttggc acgccttgaa tttgatattg accggctact cccggatttt tatctatatc
    11041 gaccttaatc acacgcacct taccagattt ttctcctgcg aaatcgcgca atatcggtgc
    11101 catcgtttta catggaccac accattcagc aaaaaaatct actaaaacag gctgatcacc
    11161 attaatcaat tcgctaaatg ccgaagttgt tgacatatta atacaatgtg caaaggaagg
    11221 agaaagttca acgattcacc gtgacttttg tcacgacagc aagcctgctc taaaaaacag
    11281 ctcgtttcct gctcgtgctg ggtgactatg ttcgcatgga atcatttcga taatgttgag
    11341 tgatcctgca aatacctccc gatattcggc ttctgtgccg ccatacggtg gaccgggttt
    11401 atcaaatggt gttttgaata ataatcctgc gagttcacct ttgggtttta acagttgtgc
    11461 catatgtttt acataggccg gtcgcaatgc aggatctatg gcgcaaaaga aggtctgttc
    11521 tataatgtaa tcgtattttc cggcgtgtgc gaagaaatca cctaatacta cattgcactg
    11581 ttgttgagca tcagcgccga gtgcttcgcg taaatgattt acaggaggtt cggcaatatc
    11641 gataacggtg atattggtaa atccttgttc tacaagatat tttgcttccc aggcattacc
    11701 gcatccggga attaatatgg ctgcatcttt tctttcaatg ctatcgaaat agtgttttaa
    11761 tgccggagca accacgccca tatcccaacc tgttgtgcca tcagcataac gctgctccca
    11821 atactctttt gataagtgct ctgtcataca aataaattaa tgagctctat ttcgttgcga
    11881 tgcaatttta tcttgccgtt ttgttccatt tttttcagca atcttgaaat cacctcacgc
    11941 gtggaactca ggtcgttcgc tatttgctga tgtgttattt caagacggcg agaattatat
    12001 tcattgaact gattgaataa ataatgttcc aatcgttcat ccatatgttt gaacgcaatc
    12061 tgatcaataa caaccagtaa ttcttcaaaa cgtgaacggt aagtctccaa tacaaagtag
    12121 taccacgatt tatattcacg catcaagtca tccatgatct ctagcggaac ctgaataacc
    12181 tgtgtatctt caacggctac agcagtaatc tcacttgcct gattgcgggc agcacatatc
    12241 atcgacaagg cgcaagcgtt tccgggttcg atataataca tgaaatactc cttaccatca
    12301 ttgctttcgc gatagagttt tacgcgtccc ccaacaatga gaatggtcga tttaaaatat
    12361 cgcccggaat acatcattac ctcgtcgcgg gcaaatgtgc gcaacgtagc taccgaggcg
    12421 atgcggtcaa caagggcatc ttcaaattgc ggaaaatgat tgtccagtag agctctgata
    12481 tcatccatac aacagcaaat ctacaacaag gaacaattat tcctgtttac cggttcggaa
    12541 tgcttttatg agtagagcag caatcaacaa aagccagggc gtaccgtgca gaaacacatc
    12601 aaaccagtca atcgcctgca taccaacagc acctcctgca atccaacgta ttttacccca
    12661 gatatgcggc tcgggagtga atggagcgag tcctaaggtt aaagatgcca tcaggtaata
    12721 ttgccaaccg gcagcaaaac gcttagccca catgcgaacg gagattatac caacttccgg
    12781 cattgtaaac ctcagtaaag ccatgcgatt tcagtatcga ttttgccgaa ccactgcgca
    12841 tgcctgaagc acaacataca ataatgggac ctttgttttt aatttttttg atgcttccct
    12901 ccagtttatc cagcgggata ttgatagcgc ctttcgcgtg accaccatta aattcaccaa
    12961 cagtgcgcac atcaataatc accgctccat ccttcaccag ttgtgcataa tccaccttcg
    13021 ggccaccacc taacattttt ttgagtaagt ctatcatcct gtaatttttg cccaaaggta
    13081 gagccgcaaa ttgccctcgt cagtgacaaa tattactcgt gaatcgtgaa tcgtgaatcg
    13141 tgagtggtca gtggtgggtc gtgagcttgc cgataggcgg agttgcccat tgaccaatgt
    13201 gaactgtgat tccgggtaag gtatcaatgg aataatgtta ttaatcaaac aatccttaac
    13261 tttatttaaa atatacgtga atttgaggca tcccaccctc gaatcaaccg tgaagctata
    13321 tcagacacgt taaacctatg aaaacaaaat tctccttcga aacttataca ctgttggcat
    13381 ctgcatttct tgggatcaat agatcgggtg ctcaagtggt catcaaagac tacgaccctg
    13441 atcttcgcat ggttaaatgg gatgacactt tcaatttaca tgatacagtt aaaattgata
    13501 tcgacgaaaa tggcattaac gacttgagtt ttatatatga ttacagtggc ttagatgtaa
    13561 taatagagat tgaaattgcc gacatggtta ccggttttgg agcggaacac gatttttctt
    13621 acgacctgtg ggcacttgat gtatatgcat ttcacgaagg cgatattgtt gatgaatctg
    13681 tccattttaa agatgacgga caaaacgtag catcagcatg ggtaacattt acagaatccg
    13741 gatattattg gaatgtgtat tatcgtggaa atttcaattc atatggatat tatttgccat
    13801 tagaattgaa tattgacgac tccatacatt acggatgggc gagattgtca atagggccat
    13861 taggaaatga tctgtataat gatatgtggt atactacaga ttattcactt gtagtgtatg
    13921 aggttggata tgaagcatcg ccaaatacac ctatcgtttg tcaaactgaa tctccaattg
    13981 aagacattaa cccgatttta aaaaatgaga ctgatggaga tgacttttca gagttcacat
    14041 atttttatca ctcagcctat tttacagatt atgccgaact cagaatattt ttaattgaca
    14101 attacaacga cgcaaaaaac ttttcagtcg cccaagccaa attgatcgat ccagcaaatt
    14161 atgaagtgat ttctggcgac accgtgcctt catataccga catatacaag cactttgatg
    14221 ccgatacaag aaccataaat ggtgacccat tcgacccgaa caaatactac gttgcctatt
    14281 tcatgaaaat tcctctggat ggagatacta cccatttaac actgtcaaca cctagtaagc
    14341 taatcaaagc ttctaagaat gcgtgcactt tgtcgcttga atccagtatc accaaagtga
    14401 atacaacagg cacagccgca gattttgatg ttactttctc gaaagatgct gatgagagcg
    14461 acatccaaga ataccgagta ggattgatca gcgaccctac cgaagttttt gatctcgcaa
    14521 atatcgtatt agataactat accttaagtg tgccgatcac cggtgcatca gaatacacgc
    14581 ttaatctgga tggaataaac tatgaactga atggaagttt gatcttcttt gatcagtatt
    14641 accatgctgt aatagctgct gttagtgatg gatatttatg tgatatttca tgctatgtgg
    14701 taacgccgaa taatgccaaa acgctcatac ccaacacagc tcaatcgcct ggcgtcactt
    14761 tgaccaatta cgcgaacttg ctaaccattc aaatcgatca accgttgcaa tcagaacaag
    14821 cacatttggt attatcgtca attgcaggag aaaaggtaat tgataccgaa ttggttgcag
    14881 ctataactga cttcaactta aacgaacttc caacgggaat ctattttgca actatatatt
    14941 gcaagggtca acccattgcg tcaaagaaaa taatcgtaag ccttacgact aaaggtagcc
    15001 attgaggccg tcatcatctc acttcgccca aatcaaaccc tgcgatcttc gcgtcctccg
    15061 cgcttaaccc gcaaattcat atagaatcat atatcccgat ttttgaggta gcccggaaaa
    15121 acagttactc ataattaaac ccggcgccgc aaaccaacat ttttcctgaa ttcagtgatt
    15181 tgtagaattc attatcaatt gaacatactg attgccaaat acttatgatt agagattttg
    15241 tgattaagcg ctgaggacac cgggggcgca tcgcatgttg ggtcaacttt ctggagttcc
    15301 agttaggtga taccacaata taggtattat ttagattttg tctaaataat attggaaact
    15361 gccccgccgg aggatatttt tgccgccaac aggcgaaaat tcaatgaaaa gaggctcatt
    15421 attgattgcg ataccctttc tgggcatttc tgcttacgga caaactattg attctgcaaa
    15481 agtccaatcg gtgctggata agctggaatt ggatgtgtat ggtgaaatat tctacaatgc
    15541 ttatcaatgg gaaaccttcc cgaaaaagaa caacgatata gacctgagca ggttggtgct
    15601 ttatccggaa tatcatttca atgatcaaat tggtgtggag atggaaatcg agttcgagca
    15661 cggaggaaca ggtgccacca tggaatatga ttacatagag gaatatggcg aatttgaaat
    15721 ggaaatagaa aaaggcggcg aagtatcgat agaagagttg gaattgaatt acacccctac
    15781 cccatggatg gaaattatag ccggaagaat aggtttgccg attggattaa ttgccggcga
    15841 cgataaacca ctgaattatt tcacaaccac ctacaacaat gttgaggcga ctttaatacc
    15901 aacggcctgg tatgaatttg gtgcaggggc agaatttaat ttccggaatt gggaagcaaa
    15961 gcttgttgtg gtgaatgccc tgaacagctc actgtttagt tctgccaact ggataaaacc
    16021 gggtaacgct atgcgcttcg agactactgc tgcagatgca tttgccgtat gtctgggaat
    16081 taattacaat ctcaatgaaa acagtcacat cggtgtaagt acttatcgtg gcaatagtac
    16141 acccaatcgt ccgaaggaag atatatcagt tgatggcaat gtttcgattg cagatgcaca
    16201 tgcgaagttg caattcggaa gatggaaaat aaatggtttg ctgatatacg gtcatcttca
    16261 aaatgcagat ctcatcagcg aagcaaacag gaatctgagc aacaacctca acgccaaacg
    16321 cacccctgtt gcaagtgaaa ccatgggtta ccttggcgaa attgccttca acatactgcc
    16381 aaaaacaaat gaacattata gagccctcga catttttgcc ggatattatt attatgactc
    16441 gatgtatggc actaccggcg acatattcaa taatccaaga tgggagaaaa cagaatggcg
    16501 cgggggattc tgttttacat ggaatgataa tatcgccatc aaatcagatg tcacctatcg
    16561 cacattgaat attcccgatg taaatacaga aatcacttac acaacagctc ttgcatttca
    16621 attttaaaat aaacactatg ttcaatatac aactaaaaca cctcctcaca cttccggcaa
    16681 ctgcaataat aatatcgtca tgcaacaata atgacgatcc ttcagatatt ggcacctatg
    16741 atttttcacc gatattaaat cacgtgtcgg aaaatgtaat taccgctact tatggcgact
    16801 tacacagcaa atcgcaatca ttgatcacag cagttgaaaa ttttaatgcc gacagatcgt
    16861 caaccaattt agaagcggca cgtcaggcat ggcgcgattg cagaattcca tgggaacaga
    16921 gtgaaggatt tttatttggc cctgtttcta cagaaggaat agatcctgct attgacagtt
    16981 ggcctgtaaa tgttattgat cttgatgcag tactggcgag tggcgaatca cttacaaagg
    17041 catatgtaga tgcacttgaa ggtacattaa aaggttttca caccattgaa tatttattgt
    17101 ggggtacaga cggcgataaa tcggcagcag catttaccga tagagaattt gaatatttaa
    17161 gtgccgtaac tcagagtctg gaaggcgcta cacaacaatt atacgatgcc tggcacagtg
    17221 gtggcgataa tttccaatta aacattgcgc aagcaggcac tgcaaccagt atttatccca
    17281 gtcaaaaatc tgctatgcaa gaattagtaa atggcatgat cggcatagca gatgaggttg
    17341 ccaacggaaa aatcaacgag ccgctcacga atatggatgt caccttggaa gaaagtcatt
    17401 tcagttctaa cagcaaggct gattttatgg ataatatccg cagtataaaa aatgtgtatc
    17461 tgggtagcta cctcatggat ggtgaaggca tcagcgatta tatcaaatca ttaaattcat
    17521 cggtagatga cgatgtgatt gcgcatatcg acgcagccat tcttgccata gaaaatattc
    17581 ccggcgattt ttcagatgcc atcttcatga atacagcatc agtagaagca gcgcaacaaa
    17641 gtgttcgtga tttgcaaagc atattggaat ctgccgttaa gccattgatc gataattatt
    17701 aatcaccgat gaaatccatt aacccgatgc gtcgatcact tgtggtgatg agtattatta
    17761 cctcaggcat tattttcctc actattcatg catgctccat cgacccacaa gtatatggcg
    17821 aatggtataa tgaagaagaa tattatgccg gtggtgagac aactatattc gaagctacca
    17881 gtggcgcttt ctctactcct gctccgaatt tgtcaccatt gcaactcgaa aaacatcttt
    17941 cgggcgatat ggaatttgag gcaacatttg tgacggcacc ttcaccggta aatgcgggac
    18001 ttggacctgt atttaatcag acatcttgcg taggatgtca tactttagat ggcagaggag
    18061 ataaacctac tgtttttaga ataagcactc cgggaacaga tgcacatggc ggaccacttg
    18121 gtgttgcagg atttggcgga gcgatacagg acaaagccat atttgacgtt gcaccggagg
    18181 caatggttga tatcagttat gttgaatcaa catatagttt tcccgatggc gaaacttaca
    18241 gtctgcgaaa accgatttat acaataatca ataattatgc tccgcttccg tctgatgttg
    18301 ctttatcggt gcgtgtggcg ccacctgtat tcggacttgg attgcttgaa ataattcctg
    18361 aagaggcaat tatcgctctt gccgatgaac aagatgccga taaaaatggc atttccggaa
    18421 aggctaatta tgtatatgat gtgcgcaccg gattgatggc tttggggcga tttggatgga
    18481 aagcaaatca gccaaattta gatcagcaaa atgcagcagc tttcaatgaa gatatgggaa
    18541 taaccaatcc aatttttcaa actgaatcct gttttggaca attacaattc gatggcataa
    18601 ccgatgaacc tgaaatcacg atgccggttt tggaggatgt tactttttat gtgcgcacac
    18661 ttgccgttcc tgcaccgaga aatctcgaag atgcaaatat tcaggaaggg aaaatgtatt
    18721 tctataaagc cggttgcaat acctgtcaca atccatcttt tacaacaggc attgctatta
    18781 atgcgcccga gctttccaat caaaaaatct atccgtatac cgatatgttg ttgcatgata
    18841 tgggcgatgc gatgggtgat ggccgacagg attatttagc cacaggaaat gaatggaaaa
    18901 cgcggccgct ttggggaata ggattaacac aacttgcaaa tggtcacacc gtttttcttc
    18961 acgacggacg tgcacgcaat ctcaccgaag ctattatgtg gcatggtggc gaagcggagg
    19021 catctacgga atatttcgca tcactgacta aggagcagcg tgataagttg ctggaatttt
    19081 tgatggcatt gtgatgcgtg aatcgtgagt cgtgagtcgt gaatcgtgag attgaatttt
    19141 ctttgcgact ccgcatcttt gcgactttgc gcgaaataac actgcaaatt ttatggtatt
    19201 aaaaatctac tttccataac tataattcac attcaaataa aaaagcctgc ttcatattta
    19261 tatgtctatt atcatgtgtg atgtatgcag aatgttttgg tttgatgatt tgaatattat
    19321 ttgaagatgg cgagaaggaa acaccgttga attacaaccc tctgtttaag cgcggaggac
    19381 gcggaggtgc gcaaaggaaa atgcagaatg cgttggtttg atgacttgaa tattatttga
    19441 agacggtgag aaagaaactc ttatgaattt caaccctctg tttaagcgcg gaggacgcgg
    19501 aggtgcgcaa aggaaaatgc agaatgcgtt ggtttaatga ttacttgacg atgttgagga
    19561 attacctctg atgaattacg cccatcaatt taaatgcaaa gcatttcaca gtaacgtaac
    19621 tacagtaatc aacgggcttg tctcaatact aactacccac tacccgccta tccgcaacgc
    19681 aagtatatca cacttgcaca cttgcacact tgcacacttg cacacttgca tacctgcaca
    19741 cttccacact tgcacacttg catacctgca cacttccaca cttgcacact tccacacttg
    19801 cacacttcca cactttttca gtggagttgg tgggattatt tgcatgatcc cttcgtgggg
    19861 aggctacaat aaaaaaacca aaaccgcttc gctataaatg caaatgccaa ttttcatcgc
    19921 ttgtaaaatt tacagactct gaaaatcggc atttgcattt acttttgctg aggcacaagg
    19981 ttttggtttt tttattgtgg agttggtggg attcgaaccc acgtccagac aaggcattag
    20041 caagctttct acatgcttag tgcgctcttg attgtcgggt tatgcaaggt gagaacactt
    20101 acctgagtat aaccttagct tcttgatctc atcctgcatc gaagcactgc aggaccagtc
    20161 cggtgagatg acgttcgtcg gaaccgtacc agacaaacgg ttaccgggaa cgaaggctac
    20221 tctaattctc tgaattaagc ggccaaggcg tagttagact cgccatgtaa aaaaagggtt
    20281 gtagcttgag attaaagagc caaattacaa tgctctgcat gcttacctgt caatgaacga
    20341 tgctgtcaat tccggtcaac cccgaatgat cattaaaccg caaaactaca tggaaaagtt
    20401 cattttgcct cccaatcggc tatatttgca gaaatggctt cagacaacac gaataatgcc
    20461 acccagcgtc catatccgag aatcattccg gatattgaca attggcctat atataccctc
    20521 agcaaggacc gtgaacagtt gatagaatcg gtgatcgacg aaacactcga aaatatcctc
    20581 aagaagaatc ctaccgagaa agacatacac gacgagctag ccaaagccgt ttacctcgaa
    20641 aaaatacgtg tagaacgccc ctggaaaacc gatgccccgg atgaaaaaac tttctggaat
    20701 ggggttaaga acaaattggt tcaactagaa agtcaccccg acaataaaat cgatgaggaa
    20761 aaggcaattt tgcgcaccat cattcatcgt tatgtgcacg agatagtcgg gaacttcaac
    20821 aaaggcactt acaattttgc cgagcgattt gtgcctttcg ccttcaaccg attactcaat
    20881 gcctcccata gcagagcttt taccagattg tttggcaaca aacgcgattt actgaaaagc
    20941 attcatctta tcggtgaaat tgaacaagtg cgcgagcttg ctaaagatgg aattattgtt
    21001 gccgttccta cacatagcag caatatcgat tcaataacag ttggatgggg aatttctgcc
    21061 atcggactgc cggccatgtt ttatggtgcg ggattaaatc tgttctcagt gcgatttctt
    21121 gcctatttta tgaatagact cggtgcttat cgcgtcgacc gcaggaagaa aaatgctgta
    21181 tatcttgaag ccctaaaaac ttattccaca caagtaataa ccaagggcgg acatagttta
    21241 tttttcccgg gtggaacgcg atcgcgcagc aatgttgttg aatcgcaatt aaaactcgga
    21301 ttgctgggaa cagcgctgga agcccaacgc cgcctcattc ttgaaacaaa tggccagtcc
    21361 tttaaaaagg tgtacgttgt acctgttacc atgaattatg gctttgtttt ggaagctaag
    21421 gaattgatag atgaacatct gaaaaatgtt ggcaaagaac gatatttcgt gccttcggat
    21481 aaatcgagtt cctctttcaa aattctaaaa ttcatgtatc gcttttttac tgccgatgct
    21541 gattttacca tcgccttcgg caagtgtatg gatatatttg gaaatcctgt cgataagcaa
    21601 ggtaatagtc tcgatcacaa tggcaacatg atagacattc gcgattattt ccgctcgcat
    21661 ggacaactta ccaatgatac acagcgcgat tctgtgtata cgaaaatgtt atccgaagca
    21721 attgttgttg cattcaaaaa gaataatgtt gccttgcctg aacatgtcat cgcatttgcg
    21781 gcatttaaat tatttcagaa aaaacatcat accagcgatc tctattccgt attgcgcatg
    21841 gctgaagaat tcagggaact tactcgcgaa gaattgaagc aagctgttgc atccattgtc
    21901 aaggaattaa gtaaactgga acacaaagaa gaaatacgtg tagatccgat tgttgctgca
    21961 gaagatctcg atttattgat tgattatgcc atcaaaaaac tgggtgtata tcatcagaaa
    22021 cgaccacttc tgaaaacacg caaaggcact atcaaatgcc aggatatgaa attgttgcta
    22081 ttttatcata atcgcctcga aggatacgga ttagaaaaac atgtctgaca aaaccagcgt
    22141 tggagttgta ggttccggaa gcttcggaac ggcggtagcc aatcttctgg cggaaaacag
    22201 tcatgtgttt ctgtattcac gtcgtgccga caatgtggta tccatggcaa atgcacgcat
    22261 atcgtcaggt caacaattac atcccaatat cacccccacc gacgatattg gtctgattgc
    22321 aaaagaatgc tatgtaatat ttcccattgt ttccagcgat ggttttaagg aagtgatcag
    22381 aaccatggct ccttttttgc gtcctgatca tattttaatt catggcacca agggtttagc
    22441 attaaagaaa aaaggacttg aaaaactgaa agaaaaatcg cgcatatcca atcgcaatgt
    22501 gcttaccatg agtcagctca ttcgcagaga aagtgttgtg gtgcgtgtcg gcagtatggc
    22561 tggtccaaat ctggcaagag agattgcaca agaaatgccc gcggctactg ttattgcaag
    22621 cagatttgat gaggttattc acgaaggaca tcgtttgctt accagtccga ggtttcaggt
    22681 ttatggttct tatgatatt
//
LOCUS       DAOQHY010000038        22619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1021_length_22619_cov_6.051498, whole genome shotgun sequence.
ACCESSION   DAOQHY010000038 DAOQHY010000000
VERSION     DAOQHY010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 22619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22619
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1021_length_22619_cov_6.051498"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(368..1180)
                     /locus_tag="PLL28_04885"
     CDS             complement(368..1180)
                     /locus_tag="PLL28_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68688.1"
                     /translation="MLKSSSKLVTIILIAISSCSPPENSEPTNDYEKFVDGLVNLQEQ
                     AEFNFDSTIVGGNCCDFDFDTYFKYFDQLSVDSNWVLESHYRISAFRGHPVVASFARN
                     DTIAAEIRRELSLSRKGLLKGKEDYDLSLRLFGYMDSVGFLSAIHINSDSGYFQYLIF
                     ALFGGQYCLFGDANYADWEIITSRQKLKYFTELENDTYYRFSKSVKKQALKIDPEIKM
                     ETFPDSITFRIVTLDPWTGFVENKYSITKSWPHKLTQYEHKVLVKYDCGVRF"
     gene            complement(1431..3206)
                     /locus_tag="PLL28_04890"
     CDS             complement(1431..3206)
                     /locus_tag="PLL28_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF68689.1"
                     /translation="MAEKKEKRSKLFSGEDYKIFRRLMSYAGPFRRQFIISTLLAIGL
                     SILTPLRPYLVGITVDDYIMTHDFHGLLILTIISLVVLFAESIGRYFFLYTTNWLGQH
                     VVKNLRVKVFEHLVGLRLSYFNRTPVGISTTRTINDLETINSVFTEGAIQIIADLLTI
                     IFVITFMFITSWQLTLISLISLPLILYATYVFKEGIKSTFNEVRTAVARLNSFLQERI
                     TGMKVVQIFNAEDQEYERFKQINADHRGAQIRSVWYYSIFFPVMEVIIALATAMMVWR
                     GAHAVFDGMATLGDINAFILYLNILFRPLRMLADKFNTLQMGVVSSDRIFKILDTQEH
                     IENKGTNAPATLKGRIDFDHVWFAYQEKDWVLRDVNFSVEPGSSLAIVGATGAGKSSV
                     INILNRFYEIQKGEIRIDNNPVQDFELSCLRRHIGMVLQDVFLFTGSIHDNITLRDPD
                     INREQVIAAAKMLGAHEFIMRLPGNYDYNVMERGATLSLGQRQLISFVRALIFNPDIL
                     ILDEATSSIDTETEKVIQHAIENLISNRTSIVIAHRLSTIRRADQIMVLDKGEVKEIG
                     THDTLIALDGYYAKLHAMQFEEEPG"
     gene            complement(3243..4433)
                     /locus_tag="PLL28_04895"
     CDS             complement(3243..4433)
                     /locus_tag="PLL28_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNF68690.1"
                     /translation="MQQLQEQIKNMASEYFEDIRTCRRYIHRHPELSFFEKETAAFIQ
                     QKLTEYHIPFEANIAGNGIVAIIAGKNPSKKTIALRADIDALPITEANDVVYASSNKG
                     VMHACGHDAHTASLLGAGRILNNLRDAFEGTVKLIFQPAEEKLPGGAKLMIEAGVLNN
                     PNVDAIIAQHVFTPFKVGTVAYCFGTAMASTDELYITIHGKGGHGAYPQDTIDPVMIS
                     AQLLVALQQVVSRTVSPFQPAVLSFGKVIADGATNVIPDKAYLEGTFRALDETVRADA
                     HKKIVEIATHIAEAFGARAEVRIEKGYPVLHNDEALTRRSYDRAVAYLGQENVFITTP
                     RMGAEDFAYYSREIPACFYRLGTGNPEKGIVSNIHTPTFDIDEDALKIGMGLMAWHAI
                     GELL"
     gene            complement(4435..4821)
                     /locus_tag="PLL28_04900"
     CDS             complement(4435..4821)
                     /locus_tag="PLL28_04900"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNF68691.1"
                     /translation="MKLRSLITILAILMCAMAKAQSGNIVINDEAGAAELIEKHIMFN
                     KDHGVLPGYRIQIYSGTSLTTAKEEKSDFLQVFDVPAVIVFEAPNYKVRVGNYLNRFE
                     ANTDLQTLNLDYPDALIVKDLITVNQ"
     gene            complement(4855..5523)
                     /gene="deoC"
                     /locus_tag="PLL28_04905"
     CDS             complement(4855..5523)
                     /gene="deoC"
                     /locus_tag="PLL28_04905"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNF68692.1"
                     /translation="MSTPKNLSAYIEHTILKPDTTHAAVEQVCHEALQHHFAGVCVPS
                     YFLEQAADLLQDSDAELITVVGFPYGYNSTMSKFEEMEDAMHNGALHIDVVVNIAAIK
                     NNDWDHVETELSTLTRLAHSEDRIIKVIAESGMMSEVELDLIIRIANEVGIDYLKTST
                     GVNGTGATAEAIEYMRKHLNPAIRIKASGGIRTREQALTFIGLGADRIGASASVAIVT
                     EGNN"
     gene            complement(5534..8860)
                     /gene="secA"
                     /locus_tag="PLL28_04910"
     CDS             complement(5534..8860)
                     /gene="secA"
                     /locus_tag="PLL28_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNF68693.1"
                     /translation="MSVLTNMISKLFGGNKYEKDIKSIMPLVDDINREFAKLSTLSND
                     QLRAKTDEFKGRIKAHLSEIDNRISSLKIQSDDPTLEDVDQKDEIYKQIDALRKDRDK
                     LIEEILLKLLPEAFAVMKETARRFTENTEIISTATELDKKISVAHDFVVIDGDKAIYK
                     NSWEPAGVPITWNMVHYDVQLIGGIVLHQGKISEMATGEGKTLVATLPAYLNALAGEG
                     VHIVTVNNYLARRDAEWNGPIFNFLGMRVDCIDLYQPNSPERRNAYLADITYGTNNEF
                     GFDYLRDNMVRSIEEKVQRKHHFAMVDEVDSVLIDDARTPLIISGPVPKGGDAEVYNV
                     LKPRINKLIDAQKRIFNTSLNEAKKLIEAGKAKEGGIHLLRAHRALPKNNALIKFLSE
                     EGIRRLLLETEGVYIAEHNKRMPEIDQELFFVIEEKNNSVDLTDKGIELITEGSEDPQ
                     FFIIPDVGIQIAEIERSLRPKEEQIEMKDNLMRDFTIKSERIHSVQQLLKAYTLFEKD
                     VEYVVMDGKVKIVDEQTGRILEGRRYSDGLHQAIEAKEAVKVEALTQTYATITLQNYF
                     RMFHKLCGMTGTAETEAGEFWEIYKLDVTAIPTNRSIVRNDMEDYVYKTRKEKYLAVI
                     DRIQALVKDGRPVLVGTTSVEVSELLSRMLQLKGVKHNVLNAKQHQREAEIVTDAGKA
                     GSVTIATNMAGRGTDIKLGPGVKEAGGLAIIGTERHESRRVDRQLRGRAGRQGDPGSS
                     EFYVSLEDNLMRLFGSERIAKVMDTMGYKEGEVIQHRMITKSIERAQKKVEENNFGIR
                     KRLLEYDDVMNNQRTLVYQKRNHALSGERLTIDLNNTIYDLCEELVSTHVESKDYEAF
                     TMDVIRYFSMEPEVSKHDFENEKPLVVTQKLYHSAREFYRRKSQQVVNKVLPLINDLY
                     TTRGETLENVIVPFSDGRKGINVMVNLKRAVDTQGVEILYSIERMISLIVIDDNWKDH
                     LRNMDDLKQSVQGAVYEQKDPLLIYKFEAFEMFKKMLSELNREIVSFLFKGSVPLRTP
                     DEVRTAAPQKTQTDMSQLKTGREEAGAGPGARPNAPQAQQERRAIEPVRVEKKVGRND
                     PCPCGSGKKYKNCHGKDEVN"
     gene            9038..11557
                     /locus_tag="PLL28_04915"
     CDS             9038..11557
                     /locus_tag="PLL28_04915"
                     /inference="COORDINATES: protein motif:HMM:NF008897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HNF68694.1"
                     /translation="MPATIQQISALLGQQGQIVSPQAPVIQVLTDSRTIFSGADSLFF
                     AISGAQHNGLQYVEQAYAAGVRSFVVEKLPSTLHGDANYLLVENAIDALQIIAAAHRL
                     QFQFPVIGITGSNGKTIVKEWLYHLLKQQLDIVRSPKSYNSQIGVPLSVWKMDERNEL
                     GIFEAGISQPGEMQKLQQVIRPTHGIITNISDPHREGFENDVQKLSEKLKLFKEASLL
                     VFCADHEQISDAIKIILPNTKTIAWSARSNNADVIISDIEQLPDSTMFTLKYDEHNLH
                     FKIPYSDSASLENSIHAIVMSLVIMNERKTLGAFTISNITIQAATLPVVSMRLELKRA
                     DHNSLLINDAYSADIASLEIALKFQAQHSTGLQRVVILSDFDQSGLERQLLVGEILKL
                     LSAYQVDRIIGIGEGWHANNEIDKSRVQTFADTETCIAYLQQQPISNAVILIKGARRF
                     TLERIGQLLELKTHGTALRIHLDHMVSNLQVYRSILKPGVKTMVMVKAFSYGSGQAEI
                     ARLLAHQRVDYLAVAYADEGVDLRRQGIRLPIMVMNPEPETFDQLLDAQLEPEIYSMR
                     ILNLWLQAVQNRKAHSENKQYVVHIKLDTGMHRLGFEASDIDVLIDTLQQHPHIHVAG
                     IFTHLAATDSADHHAFTKLQLQKFESMSTRITDALGYAVIRHALNSSGISNYPEAQFD
                     MVRLGIGLYGVDPSSTVQSRLKHVSTLVTNIAQIRNIQEGESVGYSCSFIAPKPMRIA
                     TINIGYADGFARSLSNGKGKVYALGNGHHANPYCPVVGRVCMDMTMIDITHLPHLREG
                     DAVEIFGEHITISQHAEMQGTIAYEVMTGISQRVKRVYWNE"
     gene            complement(11566..11907)
                     /locus_tag="PLL28_04920"
     CDS             complement(11566..11907)
                     /locus_tag="PLL28_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68695.1"
                     /translation="MQARKLMLPAMMLTLIIIVSSCSKSIDSCSGSVDCTGVNFSETI
                     QPLASTKCAISGCHGASFDSYSNMKSLADDGQLYSQVVDRQTMPDGGIDMSCEERAQF
                     NCWIDAGAPNN"
     gene            complement(12179..12790)
                     /locus_tag="PLL28_04925"
     CDS             complement(12179..12790)
                     /locus_tag="PLL28_04925"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNF68696.1"
                     /translation="MAFQLPALPYAFTALEPHIDARTMEIHHDKHHAAYTNNLNNAIA
                     GTEWESKTIEEILANVSKISMPVRNNGGGFYNHNLFWEIMGPNGGGAPTGDLMAAIET
                     HLGGWEKFKESFSNAATTRFGSGWAWLIVTPDKKLAVTSTPNQDNTLMDIADVKGTPI
                     LGLDVWEHAYYLNYQNRRPDYISAFFNVINWDVVTAKFNSAMK"
     gene            complement(12888..13331)
                     /locus_tag="PLL28_04930"
     CDS             complement(12888..13331)
                     /locus_tag="PLL28_04930"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNF68697.1"
                     /translation="MGIIQDPDAFFMQEALKLATKALEEDEVPVGAIIVSGGQIIAKA
                     HNLVERLHDPTAHAEMQAITAACNHFQSKYLNECTLYVTLEPCPMCAAAAQWAQVGRV
                     VFGASDPKMGYRRFGHMLHPRTEVLEGVESERATAMLKQFFSGKR"
     gene            13515..14855
                     /locus_tag="PLL28_04935"
     CDS             13515..14855
                     /locus_tag="PLL28_04935"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNF68698.1"
                     /translation="MNRFAILACFLTISGWVYGQRMLSLPDCVKLATEQNITLRQGAT
                     GVEFTSKAADQSRLLLLPNLNAGASYFWNFGYTIDPVTNLPLSNNFSANSYQLTAAMN
                     LFSGGSVANTIKKSKADLEVAGMNYQSTIENVQFQVIAAYLAVMFAEEQTKVAEQKIA
                     TTELQLSNSRKLVAAGSLPEGNILAIEAQLAQDELSLIQARNQVDKTYLDLKLLLQID
                     ADEKVSVIYPDVTRFNQLLTQDAPDAKMVADYAIANKASIKTYDYQLISDELARNIAA
                     AGAYPSLSLIGQVSTNYSSASYPQFGIEADPFKEQIDNNLSQVVGLSLTIPLFNNGQI
                     LLSKQNADLAIINTQLNRELAVNTMRQNVTQAVNDLKAAQASYLASMKNYEAAVSAFD
                     FAEKKFNAGTASSFDYTNALNTKAQSESFLIQSKYDLIFKAKIVDYYLDKPIDF"
     gene            14865..16160
                     /locus_tag="PLL28_04940"
     CDS             14865..16160
                     /locus_tag="PLL28_04940"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNF68699.1"
                     /translation="MRALKIVGIIVVVLLVVVLIGNNKGWFKQGGGTPVTADKVEKRT
                     VVETVTASGKIYPVSQVAIAAEISGEIVDLPVKEGDKVKEGDLLMRINPDLYESQVEQ
                     AQAALDNTKAQLSTARARVVQAKLQNDNAKIAFDRSSQLLKDKVISQAEYEQAQLAYQ
                     TAQAEYQIAQESVTAMEYTVKSSEAMLKEMRNNFKRTAIYAPASGTVVGLNKKKGEKV
                     LGTIQMTGDQLMTIADLTNMEIQVDVSENDVLRIDVGDTAEIEVDAYLNRTFKGVVFQ
                     IANSAGSSGSVLSAVTDQATNFKVKIYILPESYKDLMDANGDKYPFYPGMSATVEIKT
                     EVSRDVLAVPIQAVTAREDTSNTDKNKPVKEIVFLMDGDKVKEVTVETGLQDDAYIVI
                     KKGLSEGDRIVSGPYNTITNVLKDGDPVSVDKKKVEPKK"
     gene            16331..16828
                     /locus_tag="PLL28_04945"
     CDS             16331..16828
                     /locus_tag="PLL28_04945"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNF68700.1"
                     /translation="MAQMEIKGAEHGKRTRKQLFVDLTPMVDLAFLLISFFMLTTVLQ
                     ENKAIRLNMPETEGPDTKISMDRLITLIANDNNRVWYYYGDKPENGAYTDLTASGVRN
                     VLYQFHDKVISEQNREPICLIKFTGKADYNSMVGLLDEMDITDYNIYAIQDLTESELA
                     IQALK"
     gene            16888..17694
                     /locus_tag="PLL28_04950"
     CDS             16888..17694
                     /locus_tag="PLL28_04950"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNF68701.1"
                     /translation="MYNPETYLNATINDIVFEDRNKAYGAYQLRKNESRYVTYAMCVG
                     LLLTLMMTVFSLKANRSEEIVTGPIVIACPTDVDFPPLVKKIELPQPELPKPVAQRPT
                     VAYTTMVAKDDNAVTDDKVTTIDDLNGKDISTKNNDLPPTDENNPLIEKRTVVTEPVV
                     KKKEIIQFPEQAPEFNGGWTALQEYIAKKIRYPKGSSYIDAEGTVYVSFVVFDDGTIG
                     EVSTVRGIGMGYDEEAEKVIASMPKWFPGKQNGNPVNVRMTLPIKFQTLD"
     gene            complement(17767..18489)
                     /gene="rnc"
                     /locus_tag="PLL28_04955"
     CDS             complement(17767..18489)
                     /gene="rnc"
                     /locus_tag="PLL28_04955"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNF68702.1"
                     /translation="MLSGFRKIYNCYLGNEQVFCRKLKSITGITPINHAVYKLAFQHA
                     SIHNSVHANNERLELLGDAVIDLVIADFLFKKYPFKSEGFLTEMRSKIVSREQLNRVA
                     LKIGLGEMMQVRKGGTDLTKSNVLGNALEALVGAIYVDAGYAKAYHFFMHKILIPHYE
                     IDELESLVVNFKSKIIEWAQKNGHEIRFELIEEHEQKGKRMYKMGLMFNAELIAENVD
                     YSKKRAEQKASEIACRKLGIHE"
     gene            complement(18494..19747)
                     /gene="fabF"
                     /locus_tag="PLL28_04960"
     CDS             complement(18494..19747)
                     /gene="fabF"
                     /locus_tag="PLL28_04960"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNF68703.1"
                     /translation="MALRRVVVTGLGALTPVGNSVHEYWEGLVAGKSGADFITRFDAT
                     NFKTRFACEVKNFDPTSVIDKKEVRRMDPFLHYAMAAVEEAVKDARVDASAVDVDRVG
                     VIWASGIGGLKTFEEEVSYFAKGDGTPRFNPFFIPKMISDIAAGMISIRYGFRGVNFA
                     TVSACASSTHSLIIAADCIRLGRADMIVAGGSEAAVTAAGVGGFNAMKALSERNDSPQ
                     TASRPFDVDRDGFVLGEGAGALILEEYEHAVRRGATIYAEYAGGGMSADAYHMTAPHP
                     DGLGAQLVMRDAFRDANLKAEDIQYVNVHGTSTPIGDPQEIKAIQSIFGEHAYQINIS
                     STKSMTGHLLGAAGAIEAIASIMALNNGVIPPTINHFTDDPVFDTRLNLTFNKAQNRE
                     LNAVLSNTFGFGGHNASVIFKKLSN"
     gene            complement(19762..19998)
                     /locus_tag="PLL28_04965"
     CDS             complement(19762..19998)
                     /locus_tag="PLL28_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNF68704.1"
                     /translation="MSEIAEKVKKIIIDKLGVDESEVVPEASFTNDLGADSLDTVELI
                     MEFEKEFNISIPDEQAEGITTVGDAIAYLEEHVK"
     gene            20125..20595
                     /locus_tag="PLL28_04970"
     CDS             20125..20595
                     /locus_tag="PLL28_04970"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HNF68705.1"
                     /translation="MLPLSCELQLVARRKLKIDPDFDFLLAGLATPLQDYRLAWWINK
                     VLHKELSRVDDLVVTDAETRRQTSFARFDYPEELTRTVFHLLQNRVGSAYLMPELKEF
                     DYLLMIKGSYYRTKQAGILKKLREIEQMQASVFINPENLRSRNHLIIETKIAGW"
     gene            20624..22051
                     /gene="pyk"
                     /locus_tag="PLL28_04975"
     CDS             20624..22051
                     /gene="pyk"
                     /locus_tag="PLL28_04975"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNF68706.1"
                     /translation="MKRGHNKTKIIATLGPVSANPEMIRKLIHAGVDVFRLNFSHGTH
                     ELHKQTIALIKEANRELNTHVSILADLQGPKIRLGEVLPLTVLKDGDIFRFTTHPQIG
                     NKEVAYITYPNFPKDVKVGETVLIDDGKIELKVLETNNHDTVVTKVVFGGPISSKKGV
                     NLPDTQVSIPSLTEKDHADLMFALGEGANWIALSFVRKAEDIIHLKNIIRHHGYHSRV
                     IAKIEKPEALKNIDEIIDATDAIMVARGDLGVEMPVELVPFTQKDLCRKCLAKATPVI
                     IATQMMESMIEHPHPTRAEVTDVANAVLDGADAVMLSAETAAGKFPEKVIKTMVRIVE
                     QAEKESVVYDRRHIPDKRSKTYLSDAICFNACNIAAEVHAAAIMGMTKTGYTAFMVSS
                     CRPKSRIYIFTNNPEVVNILNLLWGVEAFFYDGMRGTNNTFREVQQHLVEQGKLKQGD
                     TVINLGTMPVKEMARTNTLKISQIL"
BASE COUNT         6585 a         4894 c         4710 g         6430 t
ORIGIN      
        1 atccgttgcc tttggtgctt tcatttgaat taattttttt aatggtgatt attgatgatg
       61 aggtatttta atacggtcac aatttaatta tgtaaaacat gtgatccata aataattaat
      121 ttctatgctt cacctaattt aattgatatt gagcgaaaat caaagcttta acattcaaat
      181 tcacggaaaa ccgttaattt gcaaatgtca caaataatgc catttcgaaa aacaactata
      241 tatgaattga aagtgcttca agcgacacag taacaaatac atgtgttttg tgctgtcaca
      301 tttgaacaag agaattgcag cagcaatttt caacaaaaga gtttgaaacg gtagaaaaaa
      361 tgaatttcta aaacctcacc ccacaatcat acttcaccaa aaccttatgc tcatattggg
      421 ttagtttatg cggccaggat ttggtgatgc tgtatttgtt ttctacaaaa ccggtccaag
      481 ggtccagggt gacaatccga aaagttatcg aatcaggaaa ggtttccatt ttaatttcgg
      541 ggtctatctt cagagcctgt ttcttaacgc ttttggagaa tctgtaatat gtatcattct
      601 ccaactctgt gaaatatttc aacttttgcc ttgaggtgat gatttcccaa tctgcataat
      661 ttgcatctcc aaataaacaa tattgacctc caaacaatgc aaatatcaga tattgaaaat
      721 agccggaatc tgaatttata tgaattgcgc tcaaaaaccc tacagaatcc atataaccaa
      781 agaggcgtag cgacaaatcg taatcttcct ttccttttag taagcctttt ctgctcaatg
      841 ataattcacg tcttatctct gccgcaatgg tgtcatttct tgcaaaagaa gctacaacag
      901 ggtgaccccg gaaggcagaa atccgataat gtgactccaa aacccaattc gaatcaactg
      961 acaactgatc aaaatatttg aaatacgtat caaagtcaaa atcgcaacaa ttacctccaa
     1021 caatggtact atcaaaatta aattcagcct gctcctgtaa attcaccaaa ccgtcaacaa
     1081 atttttcata gtcatttgtc ggctcagagt tttcaggggg cgaacaggag gagatggcaa
     1141 tgaggatgat ggtaacaagt ttagaagatg atttgagcat gggtgttttg gagtttttta
     1201 accgggaaga aatattgatt tatcaccagt tacgcacagc tcactcctta caaatttaca
     1261 ctgccggtag aatgattgac tattggaaaa ccgataatat gaggtatgtc acaaaatatg
     1321 aggcgaaagc tcctgtctat cttcataaat attcagtcct agaatgtaga agacaaataa
     1381 aaccggcatt taccatgtca catttgtcta aaattatgac atattatttc tcatcccggc
     1441 tcctcttcaa attgcatggc atgaagcttg gcatagtagc cgtccaatgc aatcagggtg
     1501 tcgtgggtgc caatttcttt gacttcacct ttgtcgagga ccatgatctg atcagcgcgg
     1561 cggatggtgc tgaggcgatg agcaatcact atggaggtgc gattgctgat gagattttct
     1621 atggcgtgct gtatcacctt ttctgtttct gtgtcgatgg atgatgtggc ctcgtcgagg
     1681 atgagtatgt caggattgaa aatcaaggcg cgaacaaaac tgattaattg ccgttgcccg
     1741 agagaaagcg ttgccccgcg ttccatcaca ttgtaatcgt aattgcccgg caatcgcata
     1801 atgaattcat gggctccaag cattttcgcc gctgcaataa cctgctctct gttaatatcg
     1861 ggatcgcgaa gtgtaatgtt atcatgtatg cttcccgtaa ataaaaatac atcctgcaac
     1921 accattccaa tatgccgacg taaacatgat aattcaaaat cctgcaccgg attattatca
     1981 atgcggattt ctcctttctg tatttcgtaa aatctgttga gaatgtttat caccgaactc
     2041 ttccccgctc ctgttgcacc aacaattgcc agcgatgatc cgggttctac actgaaatta
     2101 acatcgcgca gcacccaatc tttttcctga tatgcaaacc atacatgatc gaaatcgata
     2161 cgccccttta aggttgcagg tgcattcgtg cccttgtttt cgatatgctc ctgtgtatcc
     2221 agaattttaa atatgcgatc gctgctcacc acacccattt gcaaggtatt gaatttatct
     2281 gccagcatgc gcaatggacg gaacaggata ttcaaatata aaatgaatgc attaatatcg
     2341 cccagggttg ccatgccatc aaacacagcg tgcgctcctc gccataccat catggctgtt
     2401 gcgagggcaa taattacttc catcaccgga aagaaaatag agtaatacca taccgatctg
     2461 atctgtgctc cacgatgatc ggcattgatc tgcttaaagc gctcatattc ctgatcttcc
     2521 gcattaaata tctgcaccac cttcattccg gtaatgcgct cttgtaaaaa tgaatttaat
     2581 cgcgctaccg ccgtgcgcac ttcattaaat gtgctcttga tgccttcctt gaaaacatag
     2641 gttgcatata aaatcaatgg caggctaatc aacgaaatta atgtgagctg ccaactggtg
     2701 atgaacatga aggtaatcac aaatatgatc gtcagcagat cggcaatgat ttgaatggcg
     2761 ccttccgtaa atacagaatt gatggtttcc aaatcgttaa tggtgcgcgt ggtggaaatg
     2821 ccaacgggtg ttctgttgaa gtaactgagt cgcaatccaa ccaaatgttc gaatactttt
     2881 acgcgcagat tttttacgac atgctgacct agccagttag tagtgtataa aaagaaatat
     2941 ctccctatgg attcagcaaa taaaaccaca agggaaatga tggtcaatat caacaatcca
     3001 tgaaaatcgt gggtcatgat ataatcatca accgtaattc caactaaata aggtcgcagg
     3061 ggagtgagta tggaaagacc gatggcgagt aaagtggaga tgataaattg tcggcgaaag
     3121 ggaccggcat aagacatcaa ccggcgaaat atcttataat cttcaccgct gaataacttt
     3181 gatcgctttt cttttttttc cgccacaata aattaaatgg tgcaggcaca acaacgtacc
     3241 tgttacaaga gttcaccaat agcatgccac gccatcaatc ccatacctat tttcaatgcg
     3301 tcttcatcga tatcgaaagt tggggtatgt atgtttgaaa cgataccttt ttccggattt
     3361 cctgttccca accggtaaaa acaagccggt atttcccggg aataataggc aaaatcctca
     3421 gcacccattc gaggtgtggt gatgaatacg ttttcttgtc cgaggtaagc cactgccctg
     3481 tcataactgc gacgtgtcaa ggcctcatca ttatgtaaaa cgggataccc tttttctata
     3541 cgcacttcgg cacgcgctcc aaatgcttca gcaatatggg tggcgatctc aactattttt
     3601 ttgtgtgcgt cggctctaac tgtttcatct aatgcgcgaa aggtgccttc caaatatgct
     3661 ttatcaggta tcacattggt agcgccatcg gcaatcactt taccgaaact taatacggca
     3721 ggctgaaaag gtgaaacagt tctgctcacc acttgctgca atgcaacgag taattgcgca
     3781 ctgatcatca cagggtcaat agtatcttga ggataggcac cgtgccctcc tttaccgtga
     3841 atagtaatat aaagttcatc ggtgctcgcc atggcagtgc cgaaacagta tgctacagtt
     3901 ccaaccttaa atggtgtgaa tacatgttgt gctatgatgg catcgacatt tggattattc
     3961 agcacacccg cttctatcat caattttgcg ccgccgggta atttttcttc tgcgggttga
     4021 aagattaatt tcaccgtacc ttcaaatgca tcgcgcaaat tattgagtat tctgcccgcc
     4081 cccaaaagac ttgctgtatg ggcatcgtgg ccgcatgcgt gcattacgcc cttgtttgaa
     4141 gatgcgtata ctacatcatt ggcttcggta atcggcaagg catcgatatc ggcgcgcagt
     4201 gcaatggttt tctttgatgg gtttttcccg gcaataatgg caacaatacc attacctgca
     4261 atattcgctt caaagggaat atgatattca gttaatttct gctgaataaa tgctgccgtt
     4321 tctttctcaa aaaatgaaag ttcaggatgc cgatgtatat aacggcgaca agtgcgaatg
     4381 tcttcaaaat attcgctcgc catatttttt atttgttcct gcaattgctg catgttattg
     4441 attgacggtg atcagatctt tcactatcaa tgcatcggga taatctaaat tgagtgtttg
     4501 cagatcggta tttgcttcaa atcgattgag ataatttcca acgcgtactt tataattcgg
     4561 cgcttcaaaa acgattaccg caggaacatc aaatacctga agaaaatcag atttttcttc
     4621 tttcgcggta gttaaacttg tgcctgaata aatttgaatg cgataacccg gtaatacacc
     4681 atgatcttta ttgaacatga tatgtttttc gatcaactcg gcagcaccgg cttcatcgtt
     4741 gatgacgata ttacctgact gcgccttagc cattgcgcac atcaaaatag caagtatggt
     4801 aatgagcgat cgaagtttca tatcagctaa ggtagtaatt cgggcgtaaa ttgatcaatt
     4861 attgccttct gtaactatcg ccacactagc cgaagcaccg atccgatcgg cacccaggcc
     4921 tataaaggta agcgcctgct cgcgtgtgcg aatgcctccg gaagctttta tcctgatggc
     4981 agggttcaaa tgcttacgca tgtattcaat cgcctcggca gtggcacctg ttccgttcac
     5041 acctgttgaa gttttcaaat aatcaatacc tacttcatta gcaatacgaa taataaggtc
     5101 taattccacc tccgacatca tgcctgattc cgcaatcact tttataatgc gatcttcact
     5161 gtgtgcaagc ctggtgagtg tcgacaattc agtttctaca tgatcccaat cgttgttttt
     5221 tatagctgca atatttacaa ctacatcaat atgtaaggca ccattatgca tagcatcttc
     5281 catttcttca aacttgctca tggttgaatt ataaccataa ggaaaaccaa ctaccgttat
     5341 caattccgca tcactatcct gcaatagatc tgctgcctgc tctaaaaaat aggaaggaac
     5401 gcatacaccg gcaaaatgat gttgcaatgc ttcatgacaa acttgttcta cggcagcatg
     5461 cgtagtatca ggttttaaaa tggtatgctc aatatatgcg cttaaattct taggtgtcga
     5521 catgcaatct ggtttaattt acttcatcct taccgtgaca attcttgtat ttcttaccgc
     5581 ttccgcatgg acatggatcg ttgcgtccga cttttttctc cacgcgcacc ggttcaatgg
     5641 cgcggcgttc ttgttgagct tgcggagcat ttggtcttgc tcccggaccg gcacctgctt
     5701 cttcgcggcc ggttttcagc tgactcatat ccgtttgcgt tttttgcgga gctgctgtgc
     5761 gcacttcatc aggtgtgcgc agaggcactg aacctttgaa caagaaggaa acgatctcgc
     5821 ggtttaattc gctcaacatt ttcttgaaca tttcaaaggc ttcgaattta tagatcaaca
     5881 acggatcttt ctgctcgtaa actgctcctt gtaccgactg tttcaagtca tccatattgc
     5941 gcaaatgatc tttccagttg tcatcaatta cgatgaggct gatcatgcgt tcaatagaat
     6001 ataatatttc aacaccttgt gtatcaacag cgcgcttcag attcaccatc acattaattc
     6061 ccttcctgcc gtcgctgaaa ggcacgatca cgttttcgag tgtttcaccg cgggtagtat
     6121 ataaatcgtt gatcaaaggc aatactttgt ttactacctg ctgcgatttg cggcgataaa
     6181 attcacgtgc agagtgatat aatttttgtg ttacaacgag aggcttttca ttttcaaaat
     6241 catgcttact cacttcaggt tccatgctga aatagcggat cacatccatg gtaaaggctt
     6301 cgtaatcttt gctttcaaca tgggtgctca ctaattcttc acacagatcg taaatagtat
     6361 tatttaaatc tatggtcagg cgctcaccgg ataatgcgtg attacgtttt tgatacacaa
     6421 gggtacgctg attattcatt acatcgtcgt actccaacaa acgtttacgt ataccgaagt
     6481 tattttcctc cacttttttc tgtgcgcgtt ctatggattt cgtgatcatg cgatgttgaa
     6541 tcacttcacc ttctttgtaa cccatggtat ccatcacctt tgcaatacgt tcgcttccga
     6601 ataagcgcat gaggttgtct tcaagagaaa cataaaattc gctggaacca ggatctccct
     6661 gacgacctgc acgaccgcgc aactgacggt caacgcgacg agattcgtgg cgttcagtac
     6721 caatgatggc aagaccaccc gcttctttaa cgccggggcc gagcttgata tccgtaccgc
     6781 gacccgccat gttggttgcg atggttacgc ttcctgcttt acctgcatcg gtaacgattt
     6841 cagcttcacg ctggtgttgc ttcgcgttga ggacgttatg tttaacacct ttcaactgca
     6901 acatcctgct caataattca ctcacctcaa cactggtggt acccaccaat accgggcgac
     6961 cgtcttttac caatgcctga atgcgatcga taacagcgag atatttttct ttacgtgttt
     7021 tatatacata atcctccata tcgttcctga caatggaacg gttggtagga attgcggtaa
     7081 catccaattt ataaatttcc cagaactcac cggcttctgt ttctgcagta cctgtcatgc
     7141 cacacaattt gtggaacatg cggaaataat tttgcagtgt gatggttgca tacgtttgag
     7201 taagtgcttc cactttcaca gcttcctttg cttcaatagc ctgatgcaat ccatcgctgt
     7261 aacggcgacc ttcgaggata cgaccggttt gttcatcaac aattttcact ttgccatcca
     7321 tcaccacata ttccacatct ttttcaaaca atgtatatgc tttcagtaat tgttgcacag
     7381 agtggatgcg ttcactttta atggtgaaat cgcgcatcag gttatccttc atttcaatct
     7441 gctcctcttt aggacgcagt gatcgttcaa tttcagctat ttgaataccg acatcaggaa
     7501 tgataaagaa ttgcggatct tcacttcctt cggtaattag ctcgatacct ttatcggtga
     7561 ggtcgacact attatttttt tcttcgatca caaaaaacaa ttcctgatca atttcaggca
     7621 tgcgtttatt atgctcagcg atatagacac cttctgtttc taacaataac cggcgaatac
     7681 cttcctcact taaaaatttg atgagtgcat tattttttgg aagcgcacga tgggcacgca
     7741 ataaatgaat gccgccttct tttgcctttc ctgcttcaat taatttttta gcttcattta
     7801 agctggtatt aaaaatgcgt ttttgtgcat caatcaattt attgatacgc ggtttcaata
     7861 cattatatac ttcagcatca ccacccttag gtacgggacc actgatgatc aaaggtgtac
     7921 gggcatcatc gatcaataca gagtcaacct catccaccat agcgaaatga tgtttgcgtt
     7981 gcaccttttc ttcaattgag cgcaccatgt tatcacgcag gtaatcgaaa ccaaattcgt
     8041 tgttcgtacc ataggtgata tcagcaagat aggcatttct gcgttcagga ctattgggtt
     8101 gataaagatc gatacaatca acgcgcatac cgaggaaatt aaaaatcggt ccgttccatt
     8161 cagcatcgcg acgagcgaga taattattta cggttacgat atgcacacct tcaccggcaa
     8221 gagcatttaa gtaggcaggc aatgtagcta ccagcgtttt accttcgccc gttgccatct
     8281 cagaaatttt tccctgatgc aacacaatac caccgatcaa ctgtacatca taatgcacca
     8341 tgttccatgt gataggcaca cctgcaggct cccagctgtt tttgtatatc gccttatcac
     8401 catcgatcac aacaaaatca tgcgcaactg aaatcttttt atcgagttcg gtggccgtag
     8461 aaatgatttc ggtattttca gtaaatcgtc gagcagtttc tttcattacc gcaaatgctt
     8521 ccggcaataa tttgagaagt atttcttcaa tcaatttatc gcgatccttg cgcaaagcat
     8581 caatttgctt gtatatctca tccttttgat caacatcttc caatgtggga tcatcagatt
     8641 gaattttgag gctgctgatg cgattatcaa tttccgataa atgtgccttt atcctaccct
     8701 taaattcatc tgttttagca cgcagctggt cattcgacag ggtgctgagt ttggcaaatt
     8761 cgcggttgat gtcatccacc aatggcatga tggatttaat gtctttttca tacttgttac
     8821 ctccaaacaa tttggagatc atattggtga gtacactcat gctatactat tgaaggtata
     8881 tctgatgtca aaagttgttc cgaaatggcc tgcaaagata cggattaaca cccttttaag
     8941 actttttctt gccgatttgt atccgctact ccgggtattg gggtcgtgaa accacggttt
     9001 agtaaggatt gttatagctt tacagcacca aatccgcatg ccggcgacca ttcagcaaat
     9061 atcagcactc ttaggtcaac agggacaaat tgtcagtccg caagcgcctg ttatacaggt
     9121 attgacagat tccagaacaa ttttctcggg tgccgatagt ctgttttttg ccatcagcgg
     9181 ggctcagcac aatggtttac aatatgtaga acaggcatat gcggcaggtg taagaagctt
     9241 cgtggttgaa aaattaccct caacgctgca tggtgatgcc aattatttgt tggttgaaaa
     9301 cgccatcgat gcattacaaa tcatcgccgc agcacatcgc ttacagtttc aatttccggt
     9361 gatcgggatt accggcagca atggaaaaac aattgtaaaa gaatggcttt accatttgct
     9421 gaagcaacag ttggatattg tgcgcagtcc gaaaagctat aattcgcaga taggcgttcc
     9481 gctgagtgtg tggaaaatgg atgagcggaa tgagcttggc atttttgaag cgggtatttc
     9541 acaacccggt gaaatgcaga aattacagca agtgatcagg cctacgcatg gtattatcac
     9601 caatatttcg gatccgcatc gcgaaggttt tgaaaatgac gtacagaaat tatcggagaa
     9661 actaaaattg ttcaaggaag catcgttatt agtgttttgc gccgatcacg aacaaatttc
     9721 tgatgctatt aaaattatcc tgccaaatac aaaaacgatt gcatggagtg cacgtagtaa
     9781 taatgctgat gtaattatca gtgacatcga gcaactgccc gacagcacga tgtttacgtt
     9841 aaaatacgat gagcataatc tgcattttaa aattccatat agtgatagtg catcattaga
     9901 aaacagcatt catgcaatag taatgtcgct ggtgataatg aatgaaagaa aaacacttgg
     9961 tgcttttact atcagcaaca tcaccataca agcggcaacc ttgcctgttg taagcatgcg
    10021 attggaatta aaacgagccg accacaatag tctgctcatt aatgatgcat acagtgcaga
    10081 tattgcatcg ctggaaatag cattgaaatt tcaagcgcaa cacagcacag ggttgcagcg
    10141 cgtagtgata ttatcggatt ttgaccaaag cggattggaa cgccaattat tggttggtga
    10201 aatactaaaa ttactttctg cgtatcaagt cgaccgtatt attggcattg gggaaggatg
    10261 gcatgcaaat aatgaaattg acaaatcgcg cgtgcaaaca tttgcagata ccgaaacatg
    10321 catagcttat ctgcagcaac aaccgatatc aaatgccgtt attctgatta aaggtgcaag
    10381 gcgatttacg ttagagcgta tcggtcagct gttggaatta aaaacgcatg gtacagcatt
    10441 gcgcattcat ctcgatcaca tggtgagtaa tttgcaagtg tatcgctcaa tattgaagcc
    10501 cggtgtgaag acaatggtga tggtgaaagc atttagctat ggcagcggac aggcagagat
    10561 agcaaggctt ctggcgcatc agcgtgtcga ttatctggca gtagcctacg ccgatgaagg
    10621 tgttgatttg cgtcgacaag gcatacgctt gcccatcatg gtaatgaatc ctgaaccgga
    10681 aacttttgat caattattgg atgcacaact ggagccggaa atttatagta tgcgtattct
    10741 taatttatgg ttgcaggcag tacaaaacag aaaagcgcat tcagaaaaca agcaatatgt
    10801 tgtacatatc aaactcgaca ccggtatgca cagacttgga tttgaagcat ctgatattga
    10861 tgtattaatt gatacactgc aacaacatcc gcatatccat gttgcaggaa ttttcacaca
    10921 ccttgctgca acggatagtg ccgatcatca tgctttcacg aaattgcaat tgcagaaatt
    10981 tgaaagcatg tcgacaagaa ttactgatgc acttggttat gctgtaattc gtcacgcatt
    11041 aaattcttca ggcatcagta attatcctga agcgcagttt gatatggtac gattgggcat
    11101 cggactttat ggtgtcgatc catcgtcaac agtgcaatct cgattaaaac atgtgagtac
    11161 attggtaacc aatatagcac agattcgcaa tattcaggaa ggtgaaagtg ttggatacag
    11221 ttgttccttc atcgcaccaa aacccatgcg tatcgccacc attaatatcg gttatgccga
    11281 tggatttgca cgaagtttga gcaatggcaa gggaaaggtt tatgcccttg gcaacggaca
    11341 tcatgctaat ccatattgtc cggttgtagg tcgcgtttgt atggacatga ccatgatcga
    11401 catcacccat ttaccacatt tacgcgaagg cgatgcagta gaaattttcg gcgagcatat
    11461 cacaattagt caacacgcag aaatgcaggg caccattgct tacgaagtaa tgaccggcat
    11521 ttcacaacgt gttaaacgcg tttactggaa tgaataatac ggctcttaat tattcggagc
    11581 tcctgcatca atccagcagt tgaattgtgc gcgctcttcg caactcatgt caataccacc
    11641 atcaggcatg gtttgacgat caacaacctg cgaataaagc tgtccatcat cagctaaact
    11701 tttcatgttc gaatagctgt cgaacgaagc accatggcag ccgctgatag cacatttggt
    11761 agacgccagc ggttgtatag tttcactgaa atttacaccc gtgcagtcaa cagagccgct
    11821 acaagaatca atagatttag aacaagaaga aacaataata atgagtgtga gcatcatggc
    11881 ggggagcatt aatttcctcg cttgcatagt taataggtta cttgaaatca taaacgttga
    11941 gacttgaaaa aattgtatgt cgatacagtg atttattttc gacaatacaa agctgaagtt
    12001 ttcaagtatc tcaaccaatg acaaaaatta taagtttcca agaaaattga atccataaac
    12061 aacgcttctt atttagaata tgtataaaca tacaattgaa attgctattt tcttcaacta
    12121 tatgacataa aaaacccccg tatcgctacg agggtttccg gttatgttag cagatgaatt
    12181 atttcatcgc gctgttgaat tttgctgtca ccacgtccca gttgatcaca ttgaagaatg
    12241 ctgaaatata gtcgggacgg cggttttgat aattgaggta ataagcatgt tcccatacat
    12301 caagaccgag tattggtgtt cctttcacgt ctgcaatatc cattaaggta ttgtcctggt
    12361 tgggtgttga agtcactgcc agttttttat ccggagtaac aatcaaccat gcccatccac
    12421 ttccaaaacg agtagttgcc gcattgctga acgattcttt gaatttttcc caaccgccaa
    12481 gatgtgtttc aatagcagcc atcaaatcac ctgtaggagc gccgccgcca tttggtccca
    12541 taatttccca aaataaattg tggttataaa aaccaccgcc gttattgcgc acaggcatcg
    12601 atattttcga tacgtttgcc aatatttctt ctattgtttt gctttcccat tctgttcctg
    12661 caattgcgtt gttcaggttg ttggtataag cagcatgatg cttgtcgtgg tggatctcca
    12721 tcgtgcgtgc atcgatatgt ggttccaatg ctgtgaatgc atacgggaga gccggtaatt
    12781 gaaatgccat aagtatatct gttgttttgt gaataaatag ttgtttacaa agagatgtaa
    12841 agataaaaca ccagccatga aaagcagttc gctaaaagaa tattaaatca tcgttttccg
    12901 ctaaaaaact gcttcaacat agccgtagcg cgctccgatt ccacgccctc cagcacttca
    12961 gttcgagggt gcaacatatg gccaaaacgc ctgtaaccca ttttagggtc acttgctccg
    13021 aatactaccc ttcccacctg ggcccattgc gccgcagcag cacacatcgg acaaggttca
    13081 agggttacat acaaagtgca ttcgttgagg tatttagact ggaagtgatt acaagcagca
    13141 gttattgcct gcatctcggc atgcgcagtc gggtcgtgga gccgctctac caggttatgt
    13201 gctttagcaa taatctgtcc tccggaaaca ataattgcgc ccaccggcac ctcatcttcc
    13261 tccaaagcct tagtagccag tttcaaagcc tcctgcatga aaaatgcatc cggatcctgt
    13321 atgattccca tggcacaaaa ttaagcgcaa catattaatg agcccttaaa gtctctttat
    13381 aatgccaaaa aatgccttca aaagcaccta atacctgtaa ctttttgccc tgaggaacca
    13441 tttatcttta aaaagtccta atttgtcgaa tatccgcatt gcctccccaa aaagtaccct
    13501 aattttgacc tgtcatgaat agattcgcca ttttagcatg tttccttacc atttcaggtt
    13561 gggtttatgg ccagaggatg ctttcgcttc ccgattgcgt taaactggca acggagcaaa
    13621 atattacttt acgacaaggt gcaacgggtg tagaatttac atcaaaagct gctgatcaaa
    13681 gtcgtttatt gttgctgcca aatttgaatg ctggtgcctc ctatttctgg aatttcggtt
    13741 acactatcga tccggtaact aacttaccct tgtctaataa cttctcggca aacagttatc
    13801 agcttactgc agccatgaat ttattcagcg gtggcagcgt tgccaatacc attaaaaaaa
    13861 gcaaagctga tctggaagtt gccggcatga attatcagtc gaccattgaa aatgtgcagt
    13921 ttcaagtaat agccgcatat ctggcagtga tgtttgccga agaacaaacg aaggttgctg
    13981 aacaaaaaat tgctaccact gaattgcaat tatccaattc cagaaaatta gttgccgccg
    14041 gttcattgcc ggaaggaaat attctggcca ttgaagcaca gttagctcaa gatgaattat
    14101 cgcttattca ggcgcgcaat caggtagaca aaacatatct cgatctcaaa ttattgttgc
    14161 agatagatgc tgatgagaaa gtgtctgtta tatatccgga tgtaactcgt ttcaaccaat
    14221 tattaaccca agatgccccg gatgccaaaa tggttgccga ttatgctatc gcgaataaag
    14281 cttccataaa aacatatgat tatcagctta tcagcgatga gcttgccaga aatatagctg
    14341 cagcaggggc ctacccttca cttagtttga ttggtcaggt atcgacaaat tattcgagtg
    14401 cttcctatcc gcaatttgga attgaagctg atccttttaa agaacaaatc gacaacaact
    14461 tgagtcaggt agtaggattg tcgctcacca ttccgctttt caataacgga caaattttgt
    14521 tgagcaagca aaatgccgat ctcgctatta ttaatacgca gctcaaccga gagcttgccg
    14581 ttaataccat gcgtcagaat gttactcaag ctgtcaacga cctcaaggct gcacaagcat
    14641 cttaccttgc ttcaatgaaa aattatgagg cagcagtaag tgcattcgat tttgcagaaa
    14701 aaaaattcaa tgcaggcaca gcttcttctt ttgattacac caatgcatta aacaccaaag
    14761 ctcagtctga atcatttttg attcaatcga aatacgatct catttttaaa gccaagatcg
    14821 ttgattatta tttagataaa cctatagatt tttaattgaa tattatgcgc gcattaaaaa
    14881 tagttggcat cattgtagtt gtattactgg tagttgtatt aatcggaaac aacaaaggct
    14941 ggtttaaaca aggcggaggc acgccagtta cggcagacaa agttgaaaaa cgcactgtgg
    15001 ttgaaaccgt tactgccagt ggaaaaattt atcccgtaag tcaggttgcc atcgctgccg
    15061 agatctcagg agaaattgtt gacctcccgg taaaagaagg cgacaaagta aaagaaggtg
    15121 atttattgat gcgcatcaat cctgatcttt atgaaagtca ggtagaacag gcacaagctg
    15181 ctcttgataa tacaaaagca caattgtcaa ctgcacgggc tcgcgtagtg caagcgaaat
    15241 tgcaaaacga taatgcaaaa attgcttttg atcgttcatc acaattgttg aaggacaaag
    15301 taatttctca ggctgaatac gagcaagcac aattagcgta tcaaactgcg caagcggaat
    15361 accaaattgc acaagaaagt gtaacggcaa tggaatatac cgtaaaaagc agtgaagcca
    15421 tgcttaaaga aatgcgcaac aatttcaagc gcactgccat ttatgcacct gcttcaggaa
    15481 ctgttgtagg attgaataaa aagaaaggag aaaaagtatt aggaactatt caaatgaccg
    15541 gcgaccaatt gatgaccatt gccgatctta ccaatatgga aatacaggtc gatgtatcag
    15601 aaaatgatgt gttgcgtatt gatgttggtg atactgcaga aatagaagta gacgcttatt
    15661 taaatcgcac gtttaaaggc gtggtatttc aaattgccaa ttctgccggc agcagcggct
    15721 cggttttgtc ggccgtaact gatcaggcta ccaacttcaa agtgaaaatt tatatccttc
    15781 ctgaaagcta caaagacctg atggatgcaa acggcgataa atatccgttt tatccgggca
    15841 tgagcgccac cgttgaaatt aaaacggaag tttctcgcga tgtattggca gttccaatcc
    15901 aggcggtaac agcaagagaa gacacttcaa ataccgataa aaataaaccg gtaaaagaaa
    15961 tagttttctt gatggatggc gataaagtaa aagaagtaac ggttgaaaca ggtttgcagg
    16021 atgacgctta tatcgttatt aaaaaagggt tgtcggaagg cgaccggatt gtcagtggtc
    16081 catataatac tatcaccaat gtactcaaag acggcgatcc ggtgagcgta gataaaaaga
    16141 aagtagaacc gaagaaataa tccaataaat ttaattcaat gattttagcg acagttgcaa
    16201 tagtagctgt cgctattttt ttgtgtatgg catttgcagc ttgcaatttc ctattgtcat
    16261 attcctatca gatttcatat tttgatattg ccgtatatca gcctggcgtt ttattatcta
    16321 aacatgtcct atggcacaaa tggaaatcaa aggtgctgag catggtaaac gcacccgcaa
    16381 gcaattgttt gtagacctca cgcccatggt tgatctggca tttctgctga tctccttttt
    16441 catgcttaca accgtattac aggaaaacaa agccatcagg ttaaacatgc ctgaaaccga
    16501 aggtccggat acaaaaattt cgatggacag gcttattacg ctgattgcaa acgacaataa
    16561 tcgcgtttgg tactactatg gcgacaaacc ggaaaacggc gcatacaccg acctgacggc
    16621 atcgggcgta cgcaatgtgt tgtatcaatt tcacgacaaa gtgatatcgg agcaaaatcg
    16681 agaacccatt tgtctgataa aattcaccgg caaagcggat tacaacagta tggttggact
    16741 tctcgacgag atggatatta cagattataa tatttatgcc atccaggacc tcaccgaaag
    16801 tgaacttgct attcaggcat taaaataaat agacggcatt tatggaaaaa tgaatacgct
    16861 ttcatcatac cattaaatcg cacctatatg tataaccctg aaacctatct caacgccacg
    16921 atcaatgata tcgtgtttga ggacagaaac aaagcttatg gcgcctatca attgcgcaaa
    16981 aacgaatcgc gatatgttac ctacgccatg tgtgttggct tattgctaac cctgatgatg
    17041 acggttttca gtttgaaggc gaatcgctct gaagaaattg taacaggtcc tatcgttatc
    17101 gcttgtccaa cggatgtaga ttttccgccg ttggtaaaga aaattgaact ccctcaaccg
    17161 gaactaccta aacctgttgc gcaaagacca actgttgctt acacaactat ggtagcaaaa
    17221 gacgacaatg ctgttaccga cgataaggta acaaccatcg acgatctgaa cggcaaagac
    17281 atcagcacaa aaaacaatga tctgccacca acagacgaga acaatccgct gattgaaaaa
    17341 cgaacagtgg ttacagaacc tgttgtgaag aaaaaagaaa tcattcaatt ccctgaacag
    17401 gcacctgaat tcaatggcgg atggactgca ttacaggagt atattgcaaa aaaaatacgc
    17461 tatccaaaag gcagtagtta tatcgatgcc gaaggaacag tatatgtatc atttgtagtg
    17521 tttgatgacg gtactatcgg tgaggtaagc accgttcgcg gaatcggcat gggctatgat
    17581 gaagaagctg aaaaagttat cgcttccatg cctaaatggt ttcccggaaa acaaaacggc
    17641 aatccggtga atgtgcgcat gacactacct attaaatttc agacacttga ttaaatcgag
    17701 atcaatcgct gacatagaag cccgggaatt attccgggtt tctttttttg tggcagggcg
    17761 aaataattac tcgtggatgc caagtttacg gcaggcgatt tcggaagctt tttgctcggc
    17821 gcgtttctta gaatagtcga cattctctgc aatgagttct gcattgaaca tcagtcccat
    17881 tttatacatg cgttttcctt tttgttcatg ctcttcgata agctcgaagc gtatttcatg
    17941 accatttttc tgagcccatt cgatgatttt acttttgaag ttcaccacca aactttcaag
    18001 ttcatcaatt tcgtagtgcg ggatcaatat tttatgcata aagaagtggt aagccttagc
    18061 ataaccggca tccacatata tagcacctac caatgcctcg agcgcatttc ctaatacatt
    18121 acttttagtg agatcggtac cgcctttgcg cacctgcatc atttctccga gaccgatttt
    18181 taatgcaacg cgattgagtt gttcacggct tacgatcttg ctgcgcattt cggtaaggaa
    18241 accttcgctt ttgaatggat atttcttgaa cagaaaatct gctatcacca gatcgatcac
    18301 agcatcaccg agcaattcaa gtcgctcatt attggcatga accgaattat ggatagaagc
    18361 gtgttgaaag gcaagcttgt ataccgcatg gttaatggga gtgatgcccg taatgctttt
    18421 cagcttacga caaaacacct gttcatttcc caggtaacag ttgtagattt tacgaaatcc
    18481 cgatagcaag caatcagtta ctcaattttt taaatatcac agaggcatta tgaccgccga
    18541 agccgaacgt gttgctgaga acagcattta gttcgcggtt ttgtgcttta ttgaaggtta
    18601 aattgagtcg ggtatcgaaa acgggatcat ccgtaaagtg gttgatcgta ggagggatta
    18661 cgccattgtt cagcgccatg atactggcaa ttgcttcaat agcgcccgcg gcaccaagca
    18721 ggtggcccgt cattgatttt gttgagctga tattaatttg atatgcatgc tcaccaaaga
    18781 tgctttgtat ggctttgatt tcctgtggat caccgatagg cgtggaagta ccatgcacat
    18841 tgacatactg aatatcttca gctttcaaat tggcatcacg gaaagcatcg cgcattacca
    18901 attgagcgcc taatccatca gggtgaggag cagtcatatg gtatgcatcg gcactcatac
    18961 cgccaccggc atattcggca taaatggttg ccccgcggcg aacggcatgt tcatattctt
    19021 cgaggattaa cgctccggct ccttcaccca gcacaaagcc atcgcgatct acatcgaaag
    19081 ggcgtgatgc tgtttgcgga ctgtcgttgc gttcgctaag cgctttcatg gcattaaatc
    19141 caccgacacc tgcagcggta actgcagcct ccgatccgcc ggcaacaatc atgtcggcac
    19201 ggccaagtcg gatacagtcg gcagcaatga taagagaatg tgtactggaa gcacaagcag
    19261 ataccgtagc gaaattcacc cctctgaaac catagcgaat cgagatcatg cctgcggcga
    19321 tgtcactgat catctttgga ataaagaaag ggttgaaacg cggagtacca tcgcctttgg
    19381 caaagtagga aacctcttct tcaaaggttt tgagtcctcc gataccactg gcccagataa
    19441 cgcctacgcg gtctacatcc acggcagaag cgtcgacacg tgcgtctttc accgcttcct
    19501 ccactgccgc catggcataa tgcaggaatg gatccatacg acgtacctcc ttcttgtcga
    19561 ttacggatgt agggtcgaag ttttttactt cgcaggcaaa tcgggtcttg aagttagtgg
    19621 cgtcaaaacg ggtaataaaa tcagcaccgc ttttgccggc caccaacccc tcccaatact
    19681 catgtacaga gttgccaaca ggcgtcagag cccctaatcc ggtaactaca actcttctca
    19741 aagccatgag acggatctaa attacttaac gtgttcttcc aggtatgcaa tagcgtcacc
    19801 tacagtggtg ataccttcag cctgttcgtc aggaatagag atgttgaatt ctttttcgaa
    19861 ttccataatc aactcaactg tgtcgagtga gtcagcaccc aagtcattgg tgaagctggc
    19921 ttccggaact acttcgctct cgtcaacgcc cagtttgtca atgatgatct tctttacttt
    19981 ttctgcaatt tcagacattg tatttaggtt ttaatagcac tcccccttta aggcttttcg
    20041 gagtggttgt taaaaaataa agtgcaaaga taaggcaata cccgttgatt gtgcaagtca
    20101 gatgtatatc aataatgcat aggcttgtta cctttgagct gtgaattgca gcttgtggcc
    20161 cggcggaaac tgaaaataga ccctgacttt gacttcctgt tggccggttt ggccacgcca
    20221 ttgcaggatt atcgcctggc atggtggatc aataaggtgc tacataaaga gctgtcgagg
    20281 gtcgacgatt tggtggtgac agacgctgaa acccgccgcc agacgtcgtt tgcgcgcttc
    20341 gattatcccg aagagctcac ccgaacggtg tttcacttgc tgcaaaaccg cgtaggatca
    20401 gcttacctga tgccggaatt gaaggagttt gattacctcc tgatgatcaa aggcagctat
    20461 taccggacta aacaggcagg tatcctgaaa aaattacgcg aaatcgaaca aatgcaggct
    20521 tcggtgttta taaatcctga aaacctacgt tccaggaacc acctgattat cgaaacaaag
    20581 attgcaggat ggtaaatttt gataacaggc acgactatat aatatgaaga gaggacataa
    20641 caagacaaaa atcatcgcaa cactgggccc tgtgagcgca aacccggaaa tgatcaggaa
    20701 gctcatacat gcaggggttg atgtattcag gctcaatttt tcacatggca cccacgaact
    20761 ccacaagcaa acgatagcac tcattaagga ggctaaccgc gagctcaata cccatgtgag
    20821 tatacttgcc gacttacagg gaccaaaaat tcgtctcggc gaagttttac cattgacagt
    20881 attaaaagac ggcgatattt ttcgatttac tacacatccg caaatcggta ataaggaagt
    20941 agcctatatc acttatccaa attttcctaa agacgtgaaa gttggtgaaa cagtgctcat
    21001 cgacgacggg aaaattgaat taaaagtact ggaaacaaat aatcacgata ccgttgttac
    21061 taaggtggtg ttcggcggac cgatatcttc aaaaaaaggt gtcaatctac ctgatacaca
    21121 agtatccatt ccatcgctta cagaaaagga tcatgccgat ttaatgtttg cgttgggtga
    21181 aggcgccaat tggatagcat tgtcgtttgt gcgcaaggct gaagatatta ttcatctgaa
    21241 aaatattatt cgtcatcacg gttatcactc gcgcgtaatt gcaaaaattg aaaaaccgga
    21301 agccttaaaa aatatcgacg aaatcattga tgctactgat gccatcatgg tagcacgtgg
    21361 tgatcttggt gttgaaatgc ccgtagaatt ggtaccgttc acacaaaaag atctttgcag
    21421 aaaatgttta gcgaaagcga caccggtaat catcgccact caaatgatgg aaagcatgat
    21481 agaacatccg catccaacac gtgccgaggt gacagatgtt gccaacgccg tgctcgatgg
    21541 tgccgatgcc gtaatgctga gtgctgaaac tgctgctggg aaatttcccg agaaggtaat
    21601 taaaaccatg gtgcgaattg ttgagcaggc agagaaagaa agtgttgttt atgatcgacg
    21661 tcacattccg gataagcgct ccaaaacata tttgagcgat gcgatttgtt ttaatgcatg
    21721 caatattgct gccgaagtac atgccgcagc aattatgggt atgacaaaaa caggatatac
    21781 tgcatttatg gtttccagtt gcagacccaa atcacgtatt tatattttca ccaataatcc
    21841 ggaagttgta aatattttaa atctgctctg gggagtagaa gcatttttct acgatggcat
    21901 gcgtggaacg aataatactt tcagagaagt gcaacaacat ttagtagaac aaggcaagct
    21961 caaacaggga gataccgtta tcaaccttgg caccatgccg gtgaaggaaa tggcgcggac
    22021 aaatacattg aagatttcac agattttata ataatataat agcgggattt taaggcttgc
    22081 acatcacgct cggtttcgac cgcatgcgga ggaggagttt catgttatcg gaagttaatt
    22141 gcgaaactaa gttcttcatg ctttctattg atcgaattaa ttctgcaaac cacaactttg
    22201 ctccgcatca caaatgtctt ccaccttttg aagccaaaag gtggagccaa aagctcaggg
    22261 cttctgcagc tgcatgacac agccctaatg cttgcgtcat ggcccgcaag aggtcagatt
    22321 tttcaaagta cttgattatt tgatttcctg ttgtttgtac taggaatctc aatcgcagtt
    22381 attaacgact gctttcgggc ttacttggta gtcttcgatc gggattgttg accgacgcgg
    22441 cgcataaggg ctgttgtcag catctgcaaa tgccctgtcg tggcgagaga gatttttgtt
    22501 tttctgaaga atatgcgatg tgtgtttttg gcgtgtactt gatattgaag agtaaataaa
    22561 aaattccctc ggtcaacgaa aaaaccgggc atcatcaggc gtgcatctta gcccttagt
//
LOCUS       DAOQHY010000039        22608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1023_length_22608_cov_6.004212, whole genome shotgun sequence.
ACCESSION   DAOQHY010000039 DAOQHY010000000
VERSION     DAOQHY010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 22608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22608
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1023_length_22608_cov_6.004212"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="PLL28_04980"
     CDS             complement(<1..628)
                     /locus_tag="PLL28_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNF68707.1"
                     /translation="MTTRRAFIQKSVTGTALFGLGALSLESFASDHIQKLTILHSNDV
                     HSRIDPFPDDDKKYPGMGGMANKAALISKIRAQEDNVLLLDAGDIFQGTPYFNFYHGE
                     LEMKLMTAMGYDAGTMGNHDFDAGIDGFAAQLPHAGFPILVANYDFTDTVLQDKVLRY
                     TTFQKKEIKVGVFGLGIELAGLVPGSLYAQTKYLDPVIIAQEMANVLRY"
     gene            complement(618..1376)
                     /locus_tag="PLL28_04985"
     CDS             complement(618..1376)
                     /locus_tag="PLL28_04985"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HNF68708.1"
                     /translation="MHKRSSILRLLLLLAIASTLGACQHYYTDARYDMQQMRVDNLVP
                     DSATASIIAPYKNVLDAQMGAVVCTVGIDLNRAKPESLLGNAICDMMVSEVARITGEA
                     INFGVYNYGGIRQEFLNKGPLTTGKVYELLPFENFGAVVTMSGTEVAQLVQKIIDEGG
                     WPVSGGIKIIITDNKASQVFINGNLLDPNGTYRVAMNDYMANGGDNLSFIKKENVVVL
                     GTTIRDMFLHYAATLQSEGKVLESSIEGRIQYDN"
     gene            complement(1472..2002)
                     /locus_tag="PLL28_04990"
     CDS             complement(1472..2002)
                     /locus_tag="PLL28_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68709.1"
                     /translation="MELFWQILGYIIPALVVFGTTYMVIRAFLDREHKMRLLEFRQAH
                     LREALPVRLQAYERLTLFIERISLNNLLPRVRKQEMNVSEFRTALINNIRLEYEHNLS
                     QQVYVSVDAWNMIRAAKEETISVINRNAMSMLPELPGVELHKKIIAELAESEEEATSQ
                     KVLVQLKKEVMQLYGK"
     gene            complement(2192..5638)
                     /locus_tag="PLL28_04995"
     CDS             complement(2192..5638)
                     /locus_tag="PLL28_04995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HNF68710.1"
                     /translation="MRKFNYLLACLAMVLSFTAARAQLYNYTTDLGGAPASVAANTSG
                     TSLIRVNTAQAITGCPDGFNSNRFSESTTFLASRPAVEFSISPNSGYQLNITSLSVDA
                     RRNTKGPVTWRLAYSVDGGASWINNGSDYSVTVTSCGSANTLTWDIPDFSVPYTVYVR
                     VYAYGALSNLNGIATMKNIVLSGSTSFADVDADGYTSNVDCDDFNAAVNTGATEVCNE
                     IDDNCDGNIDEGVQTTFYADADGDGYGDAGNTTLACSVPAGYVTDATDCNDADAGVNP
                     GASEVCNGIDDNCDGNADEGLPFDTWYADADGDTYGDPGNSVSTCDGAPSGYVADASD
                     CDDNVASINPGASETCNGIDDDCNGMVDDGLTFDTWYADADGDGYGDAGTSVSTCDGA
                     PAGYVADATDCNDADAAINPSASEVCNGIDDNCDGNADEGLTFDTWYADADGDSYGDA
                     ASSVSTCDGAPAGYVADATDCNDADAAVNPGATEVCNEIDDNCDGNIDEGVQSTFYAD
                     ADGDGYGDAGATTMACSAPEGYVADATDCNDADAAVNPAATEVCNEIDDNCDGNIDEG
                     VQSTFYADADGDGYGDAGATTMACSAPEGYVADATDCNDADAAVNPGATEICNEIDDN
                     CDGNIDEGVQSTFYADADGDGYGDAGATTMACSAPAGYVADATDCNDANGSINPGASE
                     VCNGIDDNCDGNADEGLTFDTWYADADGDSYGDAGSSVSTCDGAPAGYVGDATDCNDA
                     DGAVNPGATEICNEIDDNCDGNIDEGVQSTFYADADSDGYGDAGSTTMACSAPAGYVA
                     DATDCNDADGAINPGATEVCNGIDDNCDGNTDEGVLITFYADADGDTYGDAAVSTDAC
                     SAPGGYVANSGDCNDASNTVYPGAAEICANGIDEDCDGVVDLPATIAAGGPTSICLGS
                     SVTLTCTTGGTGLSYQWQKNGNPIAGATGVSYVATLQGNYRCVVSKPSCSATSSPITV
                     TVLLNPSANITTPDGLDLCGKAYVRLKANTGAGLTYQWYKDGNAIGGATAVTYFATAT
                     GAYYVRVTNANGCSTNSATVNVFSSCRMAEGALAGDVNIMPNPSNGKFTINFTTAQAM
                     DASAQVSILNLAGQQVAGFEGQLIGGQMTAGVDIAPVAGAYVVVINVNGVIFTEKLMI
                     AE"
     gene            6021..6362
                     /locus_tag="PLL28_05000"
     CDS             6021..6362
                     /locus_tag="PLL28_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNF68711.1"
                     /translation="MEVLTSPITLTESAMREIRNIISEKQIPAEYALRVGVKGGGCSG
                     LSYILGFDLQKEQDELYQIDDIRLVMEKSHGMYLAGMEIDYVEGLNNRGFSFNNPNAK
                     KTCGCGTSFEA"
     gene            6463..6990
                     /locus_tag="PLL28_05005"
     CDS             6463..6990
                     /locus_tag="PLL28_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68712.1"
                     /translation="MNVRNVIISFLLVTGISAKAQPFADAVSYNDYVVNIQTTIAVAL
                     NEFNQSILVEGSTRETVEPFYNNLITVTNNSLSLIKAMPAYNGDSTLRDAASELFEFY
                     HSCFTNEYATVMTIVFDPNMTMDDYNRMNNILTDVSTRESVYDLSFQTAQYLFAIKNG
                     FTLEENEMQKTIDNN"
     gene            complement(7061..8920)
                     /locus_tag="PLL28_05010"
     CDS             complement(7061..8920)
                     /locus_tag="PLL28_05010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HNF68713.1"
                     /translation="MKRLHTLLLAVVVACSSTAVHAQIYSYTNDLSGTPSGIAANATG
                     TNLSRINGTLETTACPDGFNSSQWSKSTLLTPGKPAVEFSATPDAGFQLDVTSISADI
                     RINPKGPTIWRIAYSADGGATWITNPSDIIVESTTCDFNTNISFDVDDFSTTNTVMVR
                     LYGLGAHSSLNGKAVIRNAMIDGSVVDQDLDGDGYGFLTDCDDTNPDINPGATEICNG
                     MDDNCNGDIDEGLTFTTYYADMDGDGYGDAGMSATTCDGTPDGYVMDATDCNDADAAV
                     NPGATEICNDVDDNCDGNIDEGLAVVWYADMDGDGYGDATMMLEDCAMPEGYVMDATD
                     CNDADAAVNPGMTEVCGNGIDDNCDGNIDESDVAASFEVIGDNPTCEGTGTYFMSTAT
                     GMDITYQWYRNMMPIAGATGATYTPANAGYYQLEVSNGVCSDMTDTVHVIIKQAPEPE
                     LYMELGTDVCVNGFVKIKVIPNPGTGSTYVWYYNGDVLAGATTELVETTDAGTYYCVV
                     TGPNGCSGTSEVVTVTADCRLAGTENGTLAVYPNPAKGNFTMQLNNAPVNGEAVVHIT
                     DMTGRVVYAVNTTIVDGQSQMNVSLENNIASGMYMVTVQTQHQIWNAQISIVK"
     gene            complement(9439..11700)
                     /locus_tag="PLL28_05015"
     CDS             complement(9439..11700)
                     /locus_tag="PLL28_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HNF68714.1"
                     /translation="MATSVIGQDTLFQESFDYPALFPPMGWTNVKVAGTGAPGTWKRV
                     TTGGSPIQEPHSDPAEAEFNSYFYPSGTASDLATPVIDFTVSGTNLVSFWMYRDAGVG
                     TDKMEVYVNTTQASAGGTLIGTVNRNYLATPVEPAAGWYQYTFTVPSTYNTATNYVIF
                     KGVSGYGYNIFIDDIAILRDPVVTIPGCATSIFPANGSIDICPNLTVYWDVVPYATGY
                     KITMGNNPPNYNNVASNLDLGNALSFTAMLATGSNYKWKVKPYNVTGIAGGCINNSFT
                     TATTPCYCEADFIEPSCSSLDFIDDVYTTGAVTNFSNMNTGCSPNPGNYTYYSGTTVS
                     ANQGSTFTMNLQSGPDYPEGFAVWIDWNRDGDFNDADEYALSSAPTTSLVSNVINVPA
                     TAPLGTTRMRIRAFYNLTPPANMYCDLWNEGETEDYNINVTTCTFVTYYQDADADGYG
                     NAAVTTTSCTGAPAGYTADNTDCNDAAFSVHPGATELCNGIDENCNVTIDDNAAVAII
                     TPSGPTTVCKGASLILNANTGVGYTYQWNRNGANLAGATGSSYSVTKSGNYKVTVTVP
                     GGCTATSTVTTCTVNANPTSTISTPEGTNLCGLPDLDLVANAGAGFTYIWYKNGVIMP
                     GINTQINTVNTIGDYRVKVANAAGCSKTSAIKTVFTSCKEGASIAQGELYPNPNNGQM
                     TLSFFSPSILEGRLNVQILDLTGQVVFNEMKDLSNGSYAGTINASNLAAGLYLVKMSA
                     GDTQIQQQVIIAK"
     gene            complement(11885..14818)
                     /locus_tag="PLL28_05020"
     CDS             complement(11885..14818)
                     /locus_tag="PLL28_05020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HNF68715.1"
                     /translation="MITLLRRSLLLAIAIFALNTAQAQVNLLTQGFESLPFPPTGWSN
                     VRMTGPTLPGTWARYGNGLFPVQTPHTGSWQIRYNSHNYLAGTSGELRTAVLDFTTSG
                     TYTVSFWMYRDNWVAADKLEVYVNTTQTSVGGTLLGTINRDRNQSPVVGTNGWYQYTF
                     TIPGAFNTSTNYIVFKATSGFGNDIYVDDISVDRLAPAAPGCVTTFSPATGTTGACIN
                     QTISWGIVALASGYKLTMGSNAPNYNNVANNVDLGVALSYSTFLNPSTTYNWKITPYN
                     AFGDATGCGFNSFTTGSGTCYCTPVYLDGSCGSEDFIDDFSTSAGTTNISNNNSGCTT
                     NPSNYTYFSGMTVTATQGNSFNVSMQSGPTYAEGFAIWADWNQDGDFADAGEYAFNSG
                     VATLAVVNGSITVPYTATVGTTRMRVRCAYNYVPASDTYCTTFSEGETEDYNITVNPC
                     PATTYYADADGDGFGNAASTTLSCTGAPSGYVANNTDCNDSNASIKPGGTEVCNALDD
                     DCDASIDEGLTFTTYYQDLDGDGYGTNAATTSSCAGSAPSGYAAANTDCNDANASIKP
                     GATEICNGIDDDCDALIDDGVTFYTYYFDADGDGYGTAATTTSSCAVVPPSGYVVLGT
                     DCNDANAAIKPSALEICNAIDDDCDASIDEGLPVFTYYRDADLDTYGNNAITTSTCAA
                     APTGYVSNNLDCNDANAAIKPGATEICNSIDDDCDASIDEGVIVATITPSGPTSFCNG
                     STVTLSANTGAGYTYQWIRNGANVAGATSANYTVNKTGNYQVKITVPGGCFNTSATTN
                     VTVFAAPKATITAPGGLDLCGTGSVMLKANLGTGYTYVWKKNGVVIAGETTNVYIATT
                     TGNYRVTVTNASGCSKTSANTTVVQTCRNAVPIVGDETMEVFPNPASDIVHIHVSGIE
                     GIGEIAKIIVSDLTGQQMIVQDALIIDGDIDIDLKTNELPSGVYLVTVNGAEAQLSQQ
                     LVISR"
     gene            complement(14954..>17278)
                     /locus_tag="PLL28_05025"
     CDS             complement(14954..>17278)
                     /locus_tag="PLL28_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HNF68716.1"
                     /translation="VTYYADTDGDTYGDPFNSVTTCSGAPVGYVLDNTDCEDIDADIH
                     PGATEVCNGLDDDCNGLADDGLVFITYYSDSDGDGYGDPLSSSTGCSAPVGFVTDNTD
                     CNDANAAINPGATEICNAIDDDCDGTADDGLTFTTYYADVDGDGYGNPAVSVSTCDGA
                     PFGYVTDNTDCEDNIFEVNPATVWYLDNDADLYYTGTGVISCTSPGPGYVYAGLIGGG
                     DCNDGDTFINPGATEICNGIDDDCNGSIDDGLTFITYYADADGDTYGDAGSSVSTCDG
                     VPMGYVADNTDCNDSDAAINPAATEICNGIDDDCDGSIDEGLTFLNYYVDADGDGYGD
                     ASASPVNSCSPVAGYVTNNLDCNDSNNLISPAGTEICNGVDDNCDGTVDDGILYTIYY
                     ADADGDTYGDAGDAVSTCDGAPMGYVADNTDCNDANAAVNPGATEICNGIDDDCDGST
                     DEGLTLTTYYADVDGDTYGDAFNTTTTCDGIPSGYVVDNTDCDDTQNTVYPGAPEICD
                     GLDNDCDGEYDEGTVTATVTPSGTIAICKGDPTALNANTGVGYTYQWFKNGNVIVGAT
                     GATYFATKPGNYQVQVNIPEGCFALSAPTTVQVNPQPNANISAPNGTSLCTTVKLKAS
                     YDATYTWQWYKDGSPIVGATSYLYIATLPGSYNCQITNAYGCTRTTAALTVTACREGE
                     TVDAVVEETFELYPNPTESIFTLDMELNTTEQVASVYILNIVGEQVYAGTVGIDNGTI
                     NTTITLGNDIAAGMYVVKVIVAEKEYTKQLIIQK"
     assembly_gap    17279..17378
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17379..>22608)
                     /locus_tag="PLL28_05030"
     CDS             complement(<17379..>22608)
                     /locus_tag="PLL28_05030"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HNF68717.1"
                     /translation="YSASSNNNLFYAGTPSATNVIYYDGTTAQSTMTAYKALVSTRDA
                     ASVTENPTWQSTSGASANFLKYSVAVATQIESGGAAIVGFTDDFTGTTRNASTPDIGA
                     WELAGITADLVGPTIAYTAIPNSAVAGTQSFSSVTITDLSGVNGTSGTRPRVYYKLTT
                     NANTFNDNTNATDGWKYVEANGSTSPFDFTIDYSKLFGGAGIAIGNTVQYFVVAQDNA
                     GTPNIGINNGVFAATPTSVALTSAAFPLTGTINQFNIVSSISGTFTVCTSGCDYTSLT
                     NAGGAFADINAKLVSGNIILNINGDLTAETAANGLNEFASPYTITIQPNGGATRTISG
                     SVSSAALIKLNGADRVTINGLNTGGNALNITNTATTAPTVISLVSLGTGAGATSNTVT
                     NTTLSTGVATTIGYGISIGGSTPGSAGADNDNNTISNNTITAAPVGIYANGTASVTSG
                     GNDNLSISGNSISYNSTLSGVIGIQLANSLNATVSQNTLNITTSASTAPVGISLETGF
                     VSSSVSRNTITQILATNTGGYGGRGITVGTGTATSALTISNNMIYGVNGSNWSSFGNS
                     SSMGIAIGTIGGSSTLTTTTGGVNLYYNSVNMTGSMGSGSTTALTASLYVGSGASSID
                     IRNNVFVNTQVGTSTTQKNYAIYSVAANTAFSNINYNDYFVSNTFNTASAILGFLTSD
                     RTDLAGIIAGFGGNANSINKDPQFVSSTNLHVNTAVTSYLESAATTIAGITTDYDGDT
                     RNATTPDIGLDEGAFTAPVTNDLQATAFISPVALGSVVTGVSFTPQASFTNNGTATQT
                     SVTVRYRIVDATLTEVYNNTQVIASIASGVTTNISFASTSLAAAGTYTIYAKAELVGD
                     AVTGNDQISSTLTAEAPLCGTYAIGASQPVGYQNLTQAAAKINGLGVSCAVTFALQSD
                     YTSASETYPIVFNQYTGASAINTLTIKPATGVTATISGSLASNALIRLNGADYIIIDG
                     SNNGSTSRDLTINNTNTTAPSGIALVSLGTGLGATNNTIKNLTITTGVSTTLGYGIAA
                     GGATPGTAGDDNDNTIIQNNAITSPGTGIYVGAGATGLNNNLDINNNNITYSGSLSSV
                     IGIQVLQATGASVSQNTINITTSATTAPVGISIETGVVNSTISRNTLGTIISTNTGGY
                     AGRGITIGTGNASSNITVSNNMIYGVNGSNWSSFGNSSAMGIAIGVIGGSSTLTTTAG
                     GINLYYNSISMNGSMGSGSTSALTTALYIGSGASALDIRNNILANTQVGTSTTQKNYA
                     IYSAATSAAFTNINYNDYYVTNTFNAGSAILGNIGATDRTTIGAIQTGFGGNTNSIIT
                     NPNFVNAASNLHINTAALSPVDNTGTPVAVTTDFDGDSRSATTPDMGLDEMTSCLNIT
                     VYADADGDTYGNPAVTAIDCDGGAPPAGYVLNNSDCDDTNAAINPTTTWYLDADADLY
                     YTGTGVTSCTSPGVGYAYTGLTAGGDCNDANSAINPGATEICNGVDDDCDGSTDEGVT
                     LTFYADTDGDTYGDPASTTTACSAPIGYVANNTDCNDANAAINPGATEVCNLVDDDCD
                     GLTDEGVTTVFYADADGDGFGDAASTTNACTAPSGYVADNTDCNDALITYADLDGDTY
                     GAGAMVACGVANNTDCDDSDAAVNPGATEICNGIDDDCDGNIDEAGAVGESTWYADAD
                     GDTYGDAASSITACTAPVGYVGANDDCDDTNAAINPGATEICNGVDDECDGSTDEGLT
                     YYTYYADAEGDGFGDPF"
BASE COUNT         6490 a         4980 c         4531 g         6507 t
ORIGIN      
        1 catatctcaa cacatttgcc atctcctgtg caattatcac aggatccaaa tatttagttt
       61 gtgcataaag tgatccgggc accaatcctg ccagctcaat tcccaatccg aatacaccaa
      121 cttttatctc cttcttttga aaggtagtat aacgtaaaac cttgtcttgc aacacagtat
      181 ccgtaaaatc ataattcgcc actaaaattg gaaatcctgc atgtggcaat tgcgcggcaa
      241 aaccatcaat accggcatcg aaatcatgat tgcccatagt gcctgcatca taccccatag
      301 cagtcatcag tttcatctcc aattctccat ggtaaaaatt aaaatatggt gttccttgaa
      361 atatgtcgcc ggcatccagt aataatacat tgtcttcctg agctctgatc ttgcttatca
      421 atgccgcctt gtttgccata ccacccattc ccggatattt cttgtcgtca tcgggaaatg
      481 gatctatgcg actatgcaca tcattggaat gcaggatggt taacttttga atatgatcgg
      541 aggcaaacga ctcaagtgat agtgccccca gtccaaacaa tgctgtaccg gttaccgact
      601 tttgtatgaa ggctcttcta gttgtcatac tgtatcctcc cttcaattga tgattcgagc
      661 acttttcctt ctgattgcaa agttgctgcg taatgtaaaa acatatcgcg aatagttgta
      721 cctaatacaa caacattttc ctttttaata aaactcaggt tgtcacctcc attggccata
      781 taatcattca tcgccacgcg ataagtgcca ttcgggtcca gtaaatttcc attaataaat
      841 acctgcgatg ctttattatc agtaataata atttttattc ctcccgacac aggccatcct
      901 ccttcatcaa tgatcttctg aactaattga gcaacttcag ttccactcat tgtaactaca
      961 gcaccaaaat tttcgaatgg caataattca tatacttttc ctgtagttaa tggcccttta
     1021 tttaaaaact cctgcctgat tccaccataa ttgtatactc caaaattgat tgcctcaccg
     1081 gtaattcttg ccacttcact taccatcata tcgcatattg cattgcctag taaagattcg
     1141 ggtttggctc gatttaaatc tatccctaca gtgcaaacaa cagcacccat ttgagcgtcg
     1201 agcacatttt tgtacggggc gatgatactt gccgttgcag aatcaggcac caggttgtcg
     1261 acacgcattt gctgcatatc gtatcgtgca tcagtataat aatgctgaca tgctcccaaa
     1321 gtggaggcaa ttgccaacag cagcaataat cgcaatattg aactacgctt gtgcacgata
     1381 gtggtttgtt ttaccgccga agcagtattt cgtctttagg gcaacaaatt gggcgaggag
     1441 taaaatgcag attcagaaaa tgaattcacc atcattttcc atataactgc atcacttctt
     1501 ttttgagttg aaccaacact ttttgcgatg tagcttcctc ttctgattca gcgagttcgg
     1561 cgataatctt tttatgtagt tcaactccgg gtaattcagg taacatggac atcgcgtttc
     1621 tattgataac gctgatagtt tcttctttgg ctgcgcgaat catattccag gcatcaacag
     1681 aaacatatac ttgttgactg agattatgtt catattctaa cctgatatta ttaataagtg
     1741 ccgtgcggaa ttccgataca ttcatttcct gcttgcgcac acgtggcaat aaattgttca
     1801 gcgatatacg ctcaatgaat aatgtaagac gctcataagc ctgaagacga acaggcaatg
     1861 cctcacgcaa atgggcttgt ctgaactcca acagacgcat tttatgctct ctgtcgagaa
     1921 aggctcttat caccatatac gtagttccga ataccaccag ggccggaata atgtaaccta
     1981 atatctgcca gaataactcc atgatctttg ttcgggggtt gcaaatttaa aattaagtaa
     2041 tcactgtgca ataatgtgag aaatattttt ctttttcttt taccctctcc taaacgcata
     2101 tcggctcctt gctcgtatcc tgaaccagca ggagcaataa aaaagggtcg cccggaagag
     2161 cgacccttaa tatggttaac ctgtgaaccg cttattccgc gatcataagt ttttcagtga
     2221 aaataacacc gttaacattg ataactacta cataggcacc agccaccgga gcgatatcaa
     2281 cgcctgcagt catttgacca ccaatcaatt gaccttcgaa gccggcaact tgctgaccgg
     2341 caaggttcaa tatgctcacc tgagcacttg catccatagc ctgagcagtt gtaaagttga
     2401 tggtaaactt accgtttgaa gggttaggca taatgtttac atcacctgct aaagcgccct
     2461 cagccatacg acagctggag aatacgttta cagtagcaga gttagtgctg cagccatttg
     2521 cattggttac tcttacatag taagcaccgg ttgcagttgc aaaataggta actgcagtag
     2581 cgccgccgat agcattgcca tctttatacc actgataagt caaacctgca cctgtattcg
     2641 ctttcaggcg aacataagct tttccgcaaa ggtcaagacc atcaggagta gtgatattag
     2701 cagaaggatt gagcaataca gtaaccgtaa taggtgatga agtagcagaa catgatggtt
     2761 ttgaaaccac acaacggtag ttaccttgca atgtagctac ataagataca ccggtagcac
     2821 ccgcaattgg gttaccattt ttctgccatt ggtagctgag tcctgttcca cctgtagtac
     2881 aagtgagcgt tacgcttgaa ccaagacaaa ttgacgtagg accaccggct gcaattgtag
     2941 caggaaggtc aaccacgcca tcacaatctt catctatacc gttagcgcag atttcagctg
     3001 caccgggata cactgtatta gaagcatcgt tgcaatcacc gctgttggca acatatccgc
     3061 caggtgctga acatgcatca gttgaaactg cagcatctcc ataagtatcg ccgtcggcat
     3121 cagcatagaa agtgattaaa acaccttcgt cggtattgcc atcgcaatta tcatctatac
     3181 cgttacatac ttctgtagca ccgggattaa tggcgccatc tgcatcatta caatctgttg
     3241 catcagctac atatcctgcc ggagcagaac atgccattgt tgtagaacct gcatcgccat
     3301 agccatcgct gtcagcatct gcatagaaag ttgattgaac gccttcgtcg atgttaccat
     3361 cgcagttgtc gtcgatctcg ttacagattt cggtagcacc aggatttaca gcgccgtcgg
     3421 catcattaca atcagtagca tcgccaacat aacctgcagg agcgccgtcg catgttgaaa
     3481 ctgaagaacc tgcatcacca tagctatcgc catcggcatc ggcataccaa gtatcgaaag
     3541 taaggccttc atcagcatta ccatcgcaat tatcatctat accattacat acttctgaag
     3601 cacccggatt gattgaacca ttggcatcat tacaatctgt tgcatcagct acatatcctg
     3661 caggagcaga acatgccatt gttgttgcac cagcatcgcc gtagccatcg ccatcagcat
     3721 ctgcataaaa agttgattga acgccttcgt cgatattgcc atcgcagtta tcgtcgattt
     3781 cgttacatat ttctgtagca cccggattta cggctgcatc agcatcatta caatcagtag
     3841 catcagcaac ataaccttca ggtgcagagc atgccattgt agtagcacct gcatcgccat
     3901 agccatcgcc atcagcgtct gcgtagaatg tagattgaac gccttcgtcg atattgccat
     3961 cgcagttatc gtcgatttcg ttacatactt ctgtagcagc aggatttaca gctgcatctg
     4021 catcgttaca atcagttgca tcggcaacat aaccttcagg agcagagcat gccattgttg
     4081 tagcaccagc atcgccatag ccatcgccat cagcgtcagc atagaaggta gattgaacgc
     4141 cttcgtcgat attgccatcg cagttatcgt cgatttcgtt acatacttct gtagcgcccg
     4201 gattaacggc agcatctgca tcgttacaat ctgttgcatc agcaacgtat cctgcaggag
     4261 cgccatcgca tgtagaaact gaagaggctg catcaccgta gctatcgcca tcggcatcgg
     4321 cataccaggt atcaaaagtt aaaccttcgt ctgcattacc atcgcaatta tcgtcgatac
     4381 cgttacatac ttcagatgca ctcggattaa ttgcagcatc tgcatcgtta caatctgttg
     4441 catcagctac atagcctgca ggagcgccat cgcatgtaga aactgaagta ccggcatcac
     4501 cataaccatc gccatcagca tcggcatacc acgtatcgaa agttagacca tcgtctacca
     4561 ttccgttaca atcatcatca ataccgttgc atgtttcact tgcacccgga tttatcgaag
     4621 ctacgttatc atcgcaatca gatgcatcgg caacataacc tgaaggtgcg ccatcacatg
     4681 ttgaaactga gtttccggga tcaccataag tatcgccatc tgcatcagca taccaagtat
     4741 cgaagggtaa accttcatcg gcattgccat cacaattatc atctatacca ttacaaactt
     4801 cagaagctcc gggattaacg cctgcatcgg catcattaca atcagttgca tcagttacat
     4861 aacctgcggg aactgaacat gcaagcgttg tattacctgc atcgccataa ccgtcgccgt
     4921 cagcatctgc atagaaggtt gtttgcacac cctcatcgat attgccatcg cagttatcgt
     4981 ctatctcgtt acaaacttca gtagcgcctg tgttaacagc tgcattaaag tcatcgcaat
     5041 caacatttga tgtataaccg tcagcatcca catcggcaaa agaagtagaa ccacttaata
     5101 caatgttttt cattgtagcg ataccattca ggttactgag tgctccataa gcatatacac
     5161 gcacatacac tgtgtatgga acagagaaat caggaatatc ccatgtcaat gtgtttgcac
     5221 ttccgcaaga agttacggta acagagtaat cagaaccgtt gttgatccat gaagcaccgc
     5281 catccactga ataagcaaga cgccaggtaa caggcccttt tgtgtttctg cgtgcatcaa
     5341 cactcaatga agtgatgttg agctgataac cggagtttgg tgaaattgaa aattcaactg
     5401 ccgggcgact ggccaggaaa gttgttgact ctgaaaagcg atttgagtta aaaccatcag
     5461 gacatcctgt aatggcttgg gcggtgttga cgcggatcaa tgaagtaccc gacgtatttg
     5521 ccgccaccga ggcaggtgcg cctccaaggt cagttgtgta gttgtacaat tgcgctcttg
     5581 ctgccgtgaa gctgagcacc attgccaaac acgctaataa gtaattaaac ttcctcatct
     5641 ggtgtgggtt taggtgtgaa aaaattgctg tcaatcatgt ttccacaatt tgggggagtc
     5701 cgaagatgcg ttattaggta gtgttttccc aataatcgaa aggaatatgt aattagaggt
     5761 atgtcacaga attacatatt taaatttatt taaaatgatt ttatttacag atcattttaa
     5821 cactttttcg tttatttatt tttgaaaaga ggccattttg cctgttacag gagcatccat
     5881 tttgtaagca tttttaggca ttaaggtcaa aagtggccga ttcaaatgat gtcacaagtg
     5941 aactgaaatc tctgatttga aaccaaaaga gactcatcat cgtttagtaa ctttgtaatt
     6001 gatatcaaaa accaaatcac atggaagtat taacctcacc aataaccctg actgaaagcg
     6061 cgatgcgtga gatcaggaat attatatcag aaaagcaaat ccctgccgaa tatgcactga
     6121 gagtaggtgt aaagggaggc ggctgcagtg gattgagtta cattcttggt tttgacctgc
     6181 agaaagaaca agatgagcta tatcaaatcg acgacattcg cctggttatg gaaaagtcgc
     6241 atggcatgta tttagccggt atggagattg attatgttga aggtttgaat aaccgcggat
     6301 tttcgttcaa caacccgaat gctaagaaaa catgtggttg tggaaccagc tttgaggcat
     6361 aagctataga cttataccat tattattttg taaataaaaa atcaaaaaag atttattgcc
     6421 cggttaaatg ccgggtattt tttttaacca taactaaaac acatgaacgt tagaaatgta
     6481 attatttcgt ttttacttgt aaccggcata tctgcaaaag cgcaaccatt tgccgatgct
     6541 gtttcataca acgattatgt agtaaacatt caaactacta tagctgttgc tttgaatgaa
     6601 tttaatcaat cgatacttgt ggaaggttct acaagagaaa ccgttgaacc attttacaac
     6661 aatcttataa cagttaccaa taattcgctt tcacttataa aagcaatgcc agcatataat
     6721 ggcgacagca ctcttcgcga tgccgcttct gagttatttg aattttatca tagttgtttt
     6781 acgaatgaat acgcaactgt gatgacaatc gtattcgacc ccaatatgac catggatgat
     6841 tacaatcgca tgaacaatat tttaaccgat gtatctacaa gagaatctgt ttatgatctt
     6901 tcatttcaga cagcgcaata tttatttgcg ataaaaaatg gatttacatt ggaagaaaat
     6961 gaaatgcaaa aaacgattga taataattaa attatggaga aaataattca ataaaaaagg
     7021 tcgctcagtt gagcgacctt ttttatttat gctaatcaga ttattttaca atactgatct
     7081 gtgcattcca gatttgatgc tgtgtttgca ccgtaaccat gtacataccg ctcgcaatat
     7141 tattttcgag tgatacattc atttggcttt gaccatcaac aatcgttgta ttaactgcat
     7201 aaacaacacg gcctgtcatg tcagtaatat gcactactgc ttcaccatta acaggagcat
     7261 tatttaattg catagtaaag tttccttttg cagggtttgg ataaacagcg agtgttccgt
     7321 tttcagtgcc ggcaagacga cagtcggcag taacggtaac aacttcactg gtgcctgagc
     7381 aaccattagg gcctgttact acgcaataat atgtgccggc atcagtagtt tctacaagct
     7441 cagtagtagc acctgccaac acatcgccat tataatacca tacataagta gaacctgttc
     7501 ccggattagg aattacttta attttcacaa aaccatttac acaaacatca gtacctaact
     7561 ccatatacag ttcaggttcc ggtgcttgtt taataattac gtgaacggta tctgtcatgt
     7621 cagagcaaac gccatttgat acttctaatt ggtaataacc tgcattagcc ggtgtgtaag
     7681 ttgcaccggt tgcgccggca attggcatca tatttctata ccattggtaa gtaatatcca
     7741 tacctgtagc agtagacatg aaataagttc ctgttccttc gcaagtcgga ttatcgccga
     7801 ttacttcaaa tgatgcagca acgtcagatt cgtcgatatt gccatcgcag ttatcatcaa
     7861 ttccattgcc acacacttca gtcatacccg gattaacggc agcgtcagca tcattacaat
     7921 ctgtagcatc cattacataa ccttctggca tagcgcaatc ttctaacatc atagtggcat
     7981 cgccgtaacc atcgccatcc atatcggcat accatactac ggcaagacct tcatcaatat
     8041 tgccatcgca gttatcgtct acgtcgttac aaatttcagt agcacctgga tttactgctg
     8101 catcagcatc attacaatca gttgcatcca taacataacc gtcaggagta ccgtcgcaag
     8161 ttgttgcaga cattccggca tcaccataac catcgccatc catatcggca taataagttg
     8221 tgaatgtgag accttcatca atatcgccgt tacagttgtc atccattccg ttacaaattt
     8281 ctgtcgcacc gggattaata tcagggtttg tatcatcaca atcagtaagg aaaccataac
     8341 catcgccatc gagatcttgg tcaaccacgc taccatcaat catggcattg cgaattactg
     8401 ctttaccatt caacgaactg tgtgcaccaa gtccatataa gcgcaccatt acggtattgg
     8461 ttgtactgaa atcatctaca tcaaatgata tattggtatt aaaatcacaa gtggtagatt
     8521 caacaataat gtctgaagga tttgtaatcc aagttgcccc gccgtcggca gaataggcaa
     8581 ttctccaaat agttggacct tttgggttga tgcgaatatc cgcggaaatt gaggtaacgt
     8641 ctaattgaaa tcccgcatca ggagttgcag agaattcaac tgccggtttt ccgggagtca
     8701 acaaggttga ttttgaccat tggcttgaat tgaatccatc gggacaagcg gtggtttcga
     8761 gggtaccatt tattcgggaa aggttagtac ctgttgcatt tgctgctatg ccggaagggg
     8821 ttccggaaag gtcgtttgtg taggagtaaa tctgcgcatg gactgcagta ctcgaacacg
     8881 caaccacaac agccagtaaa agtgtgtgta aacgtttcat actttaaaat ttaaattgtt
     8941 agaatagtta tagaattaat agatgaatga gtgacaggtt ttcgttgcat gaatatattg
     9001 cataaaacag caaagtttaa ttgcaaacgg ataagtgcag ataattgttt actatttgta
     9061 tacaaaatca taataatcga tacatacgta ataaaacaaa atttggtttt tttaacgaga
     9121 ggagtccttg taaagatcag gctacaaaca acaaatctga tttgtatgca aaaggtgttg
     9181 tcgatttata agtgatcaat aagctcaatc acaaataagt caggttttca gcacatcacc
     9241 ttttttatct aatcacgctt tcatggggcg cctccgacat gcatgtttcc atgaatatca
     9301 atagccaagg tagaatgcgg gtattcaatt accaaatttt atttagaaaa aatattgtta
     9361 aatcttacga agtccaattt gtgatgtcac aaaaaaggct gccttgaaat aggcagcctt
     9421 taattatttc aaaccgcctt acttggcaat aatcacctgc tgttgaattt gcgtatcacc
     9481 cgccgacatt ttcaccagat acagtccggc agcaaggttt gacgcattga tagtgccggc
     9541 ataagaccca ttagacaggt ctttcatttc attgaaaacg acctgaccgg taagatctaa
     9601 tatttgaaca ttcaggcgac cttccaagat tgaagggctg aagaatgaca atgtcatttg
     9661 gccgttgttc gggttcggat agagttctcc ctgtgcaatt gaggcacctt ctttacaaga
     9721 tgtaaatacc gttttaattg ccgatgtttt agagcaacct gccgcatttg caaccttcac
     9781 tctataatca ccaattgtat ttactgtgtt gatttgggta tttatacccg gcatgataac
     9841 accattttta taccagatat aagtaaatcc tgctccggcg tttgctacaa gatccaagtc
     9901 aggtaagccg caaaggttgg tgccttccgg agtgctgatg gttgaagtag gatttgcatt
     9961 tacggtgcag gtagtaacgg tagaagtagc agtacatcca ccgggaacgg ttacggttac
    10021 tttataattt cctgactttg taacactata tgaagaacct gttgcgccgg caagatttgc
    10081 gccgtttcta ttccattgat aggtataacc aacaccggta ttggcattta atatcagact
    10141 tgcgcctttg caaactgttg ttgggcctga aggcgtaatt attgctacgg cggcattatc
    10201 atcgatggtt acgttgcagt tttcatcaat gccgttacaa agttcggtag cgccgggatg
    10261 tactgaaaaa gcagcatcat tacaatcagt attgtctgca gtatagccag caggtgcgcc
    10321 tgtgcagctt gttgtagtaa cagcggcatt gccataacca tcggcatctg catcctgata
    10381 gtatgtcaca aatgtgcagg ttgtgacatt aatattatag tcttccgttt caccttcatt
    10441 ccacaaatcg caatacatat ttgccggtgg ggtaaggtta taaaaggctc tgatgcgcat
    10501 gcgggttgtt cccaaaggtg cggtggcagg aacgtttatg acattgctaa ctaatgaagt
    10561 tgttggtgca gaactaagtg catattcatc tgcatcatta aaatcaccat ctctgttcca
    10621 gtcgatccaa acagcaaatc cttccggata atcaggaccc gattggaggt tcatggtaaa
    10681 tgttgaaccc tggtttgccg aaacagttgt tcctgaataa taagtataat tacccggatt
    10741 cggtgaacaa ccggtattca tattgctgaa attggttact gcacctgttg tataaacatc
    10801 atcgataaag tcgagtgaag aacaactagg ttcaatgaaa tccgcttcgc aataacatgg
    10861 tgtagttgct gttgtaaaac tattattgat acagccgcct gcaattccgg ttacattata
    10921 aggcttaact ttccatttat aattggagcc tgttgccaac atagcggtaa aactcaaggc
    10981 atttcccaaa tccagattgc tggcaacgtt attataatta ggtgggttat tacccattgt
    11041 tattttgtag ccggtggcgt atggcactac atcccagtaa actgtcaagt taggacaaat
    11101 atctatcgaa ccattcgccg ggaaaatgga ggtagcgcag ccaggaatgg taacaacagg
    11161 atcgcgaaga atggcgatgt catcaataaa aatattgtaa ccataaccac ttacaccttt
    11221 aaaaatcaca tagtttgtag ccgtattata tgttgatgga acggtaaaag tatattgata
    11281 ccaacccgct gccggttcaa ccggtgtagc aagataattt cgatttactg ttcctatcag
    11341 ggtgccgcct gctgatgcct gagtagtgtt tacatacacc tccattttgt cggtgccaac
    11401 acctgcatcg cgatacatcc aaaaagaaac gaggttagta cctgacactg taaaatctat
    11461 caccggtgta gcaaggtcgc tggctgtacc tgatggatag aaatacgaat taaattctgc
    11521 ttcagcggga tcagaatgtg gctcctggat tggcgaacca ccggtggtaa ctcgcttcca
    11581 agttccggga gcgcctgtac cggcaacctt cacatttgtc caacccatag gtggaaacaa
    11641 agcggggtaa tcaaatgatt cctgaaacag tgtgtcctga ccaattacac tagtggccaa
    11701 gaaaattccg aacacaaggg tgccgagttt tcggaggggg aaattacatg tgtgcataca
    11761 attattaaat atgaatgaat acctggatat agctgcaaag ttaaggtaca agtcgcataa
    11821 aagacctgtc ccacgcatct atatgacgca tgggacagta tgttcgttgc ctgtgttcga
    11881 agtcttacct gcttattacc aattgttgtg aaagttgcgc ctcagctcca ttcaccgtta
    11941 ccaggtaaac tcctgaaggt aactcattgg ttttcaagtc aatatcaata tcaccatcta
    12001 tgatcaatgc atcctgaacg atcatttgct gaccggtaag atcgcttact atgatttttg
    12061 cgatttcgcc aattccttcg attcctgata catgtatgtg cacgatatcg ctggcggggt
    12121 tagggaatac ttccatggtt tcatcgccaa caatcggaac tgcattgcga caggtttgca
    12181 caacggttgt attagctgaa gttttagaac agcctgatgc atttgtaact gttacccgat
    12241 aatttccggt agtggttgcg atatatacgt tagttgtctc accggcaatc accacgccat
    12301 tctttttcca tacataggta tagcccgtgc ctaagttggc tttaagcatt acgcttcctg
    12361 ttccgcaaag atcaaggcca ccgggtgcag tgatggttgc tttcggcgca gcaaaaacag
    12421 taacatttgt tgttgcagat gtattaaaac atccacccgg aacggtgatt ttcacctgat
    12481 aatttccggt tttgtttacc gtgtagttag cagatgttgc tccagcaacg ttagcaccat
    12541 ttcttatcca ttggtaggta tatcccgcac ctgtatttgc actcaatgtt actgtgcttc
    12601 cattacagaa tgatgtaggc ccggaaggag ttattgttgc aacaatcaca ccttcgtcaa
    12661 ttgatgcatc gcaatcatca tctatgctat tacaaatttc tgtagcgccg ggtttaattg
    12721 ctgcattcgc atcgttgcaa tctaaattat tagaaacata accggttgga gcagccgcgc
    12781 aagttgatgt agtgatggca ttatttccat aagtatctaa atcagcatcg cgataataag
    12841 taaacaccgg taagccttca tctatcgatg catcgcaatc atcatcaata gcattacaaa
    12901 tttcaagtgc acttggttta attgccgcat tggcatcatt acaatctgtt ccaagcacaa
    12961 catatcctga aggaggcact acagcacatg aactggtagt tgttgcggca gtaccatatc
    13021 cgtcgccatc ggcatcgaaa taatatgtgt agaatgttac gccatcatca atcagcgcgt
    13081 cacaatcatc atcaatgcca ttacagattt ctgttgctcc cggtttaatt gaggcattcg
    13141 catcattaca atcggtattt gcagcggcat aaccggaagg agctgaacct gcgcaagaag
    13201 aagtagttgc agcatttgtt ccgtaaccat cgccatcaag atcctgataa taagttgtga
    13261 atgtaagtcc ttcatctatt gaagcatcac aatcatcgtc aagtgcatta caaacttctg
    13321 ttccacccgg tttaattgag gcattcgagt cattgcaatc agtattattt gcaacataac
    13381 ctgaaggcgc tcctgtgcaa cttaatgttg ttgaagcagc atttccaaat ccatcgccat
    13441 cagcatcagc ataatatgtt gtagccgggc atggatttac cgtgatatta taatcttctg
    13501 tttctccttc actgaaagtg gtgcaatagg tatccgatgc gggcacataa ttgtaagcgc
    13561 atctgacacg catgcgtgtt gtgcctactg ttgcagtata gggaactgtg attgatccgt
    13621 ttacaacagc taatgttgct acgccggaat taaaagcata ttcacccgca tcagcaaaat
    13681 cgccatcctg attccaatcg gcccaaatgg caaagccctc tgcatatgtt ggtccgcttt
    13741 gcatactcac attaaatgaa tttccctgag tagcggttac ggtcatgccc gagaaataag
    13801 tgtaattact tggattagta gtacatccac tattattatt tgaaatattc gttgtacctg
    13861 ctgaagttga aaaatcatct ataaaatctt ccgagccgca tgatccatcg agataaacag
    13921 gcgtgcaata acaagttccg gaacctgttg tgaagctgtt aaaaccacaa cctgttgcat
    13981 caccaaatgc attgtatggt gtaattttcc aattatatgt tgttgatgga ttaaggaatg
    14041 tactataact caatgcaaca ccaagatcaa cattgttggc aacgttatta taattaggtg
    14101 cattgcttcc catggttaat ttatatcctg aagcaagtgc aactattccc cacgaaattg
    14161 tttgattaat acatgctccg gtagtaccag tagcaggtga aaatgtagta acacatccgg
    14221 gggcagccgg cgctaaacgg tcaactgaaa tatcatcaac atatatatca tttccgaaac
    14281 cggatgtggc tttaaataca atataatttg ttgaagtatt aaatgctccg ggaattgtaa
    14341 aagtatattg ataccaacca ttagttccca cgacaggcga ttgatttcta tcgcgattga
    14401 tagttcccaa aagtgtaccg cctacagaag tttgagtggt atttacatat acttccaatt
    14461 tatctgctgc tacccagtta tcgcgataca tccaaaaact aaccgtataa gttcctgaag
    14521 tagtaaagtc gagaacagct gtgcgcaatt caccggatgt tccagccagg taattgtgtg
    14581 agttatagcg tatttgccat gaacccgtat gcggtgtttg taccgggaat aagccatttc
    14641 catatcttgc ccatgttccg ggcaatgtag gaccggtcat tctcacattt gaccaacctg
    14701 ttggggggaa tggcagagat tcaaatcctt gtgtaagtaa gtttacctgt gcctgagcag
    14761 tgtttaacgc aaaaattgct attgccagca ataagctgcg tcttagtaat gtaatcatag
    14821 ccttgttgag tatgggttaa agatagtggg taggtttcgc ttccaattac taaaataagg
    14881 atattccaat ttcgcgacaa aaaaatcccg caggagttga cctgcgggat tgtaaatgat
    14941 tgtttgagaa tccttacttc tggattatca gttgtttagt gtattctttt tcagccacta
    15001 tcactttcac aacatacata ccggcagcga tatcgttgcc aagggtgatg gttgtgttga
    15061 tggtgccgtt gtctatacca acagttccgg catatacctg ctcaccaacg atgttcagga
    15121 tatatactga agctacttgt tcagtggtat tcagctccat atcgagtgtg aagatgcttt
    15181 cagttgggtt aggatacagt tcaaatgttt cctcaaccac agcgtcgact gtttcacctt
    15241 cgcggcaagc agttacagtt aatgctgctg ttgtacgagt acaaccatag gcatttgtaa
    15301 tctgacagtt atagcttccc ggtaatgtag cgatatacaa gtagcttgta gctccaacaa
    15361 ttgggctgcc atctttatac cattgccatg tgtaagtagc gtcgtagctg gctttcagtt
    15421 tcactgttgt gcaaaggctg gttccatttg gagccgagat gttggcattt ggttgtggat
    15481 tcacctgaac ggttgttgga gccgacagtg cgaaacatcc ttccggaata ttcacctgaa
    15541 cctgatagtt acccggtttc gttgcgaaat atgttgcacc ggttgctccg acaattacgt
    15601 taccattttt gaaccattgg taagtataac ctacaccggt atttgcattc agtgctgttg
    15661 gatctccctt acagatagca atggttcccg aaggtgtaac tgttgcagta acagtacctt
    15721 catcatattc accatcgcag tcgttatcta aaccatcgca aatttcaggt gcgccgggat
    15781 aaactgtatt ctgtgtatca tcacaatcag tgttatcaac tacataacct gaaggtattc
    15841 catcgcaagt tgttgttgtg ttgaatgcat cgccataagt gtcgccgtca acatcagcat
    15901 agtatgtagt taatgtgagt ccttcatcag ttgatccgtc acaatcatca tcgatgccgt
    15961 tacagatttc tgttgcaccc ggattaactg cggcatttgc atcgttacaa tctgtgttat
    16021 cagcaacata tcccataggc gcgccgtcgc atgttgaaac tgcatcacct gcatcaccat
    16081 aagtatcacc atcggcatct gcataataga ttgtatacaa gataccatca tctactgtac
    16141 catcgcaatt atcatcaaca ccgttacaga tttcagttcc tgcagggcta atgagattgt
    16201 tcgagtcgtt acaatcaaga ttatttgtta catatcctgc aacaggtgaa cagctattta
    16261 ccggtgaagc agatgcatca ccataaccgt cgccatcagc atcaacataa taattcagga
    16321 aagtaagtcc ttcatctata gaaccatcgc aatcgtcatc tatgccgtta caaatttctg
    16381 ttgctgcagg attaatggca gcatcggagt cattacaatc tgtattatct gcaacataac
    16441 ccattggaac gccgtcgcaa gttgatacgc ttgaaccggc atcaccataa gtatcaccat
    16501 ctgcatctgc gtagtaagta atgaaggtaa gaccatcatc aattgagcca ttacaatcat
    16561 catctatacc gttacaaatt tctgttgcac ccggattgat gaatgtatcg ccatcgttac
    16621 aatcaccacc tccgatcaga ccggcatata cataaccagg tcccggagaa gtacaagata
    16681 taactcctgt tccggtataa tagagatcgg cgtcgttatc gagataccaa acggtagcag
    16741 gatttacttc gaagatatta tcctcacaat ctgtgttgtc agttacatat ccgaatggtg
    16801 caccatcaca agttgaaaca gaaacggcag ggttaccata accatcgcca tctacatctg
    16861 cgtagtatgt agtaaacgtg agaccatcat ctgcagtgcc atcgcaatca tcatcgatag
    16921 cgttacaaat ttctgttgca cccggattga tagctgcatt ggcgtcgtta caatctgtat
    16981 tgtctgtaac aaatccgaca ggagctgaac aaccggtcga tgaagataat ggatcgccat
    17041 agccgtcgcc atctgaatct gaataataag taataaatac cagaccatca tctgccagtc
    17101 cattgcaatc atcatctaaa ccgttacaaa cttctgttgc acccggatgg atatctgcat
    17161 caatatcttc acaatcagta ttatctaaaa catatccgac aggggcgcct gaacatgtag
    17221 taactgaatt aaacggatcg ccataagtat caccatcggt atcagcataa taagtaacnn
    17281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg aaaggatcac caaatccgtc
    17401 gccttcagca tctgcataat aagtataata agtcagtcct tcatctgtag agccatcaca
    17461 ctcatcatca acaccgttac agatttccgt tgcaccggga ttgattgcgg catttgtatc
    17521 atcgcaatca tcgtttgcgc caacgtaacc taccggagca gtacaagccg taattgaaga
    17581 tgctgcatcg ccgtaggtgt cgccatcagc atcggcatac caagtagatt cgcctactgc
    17641 accggcctca tcgatattgc catcgcaatc gtcgtcgatg ccattacaaa tttcagtagc
    17701 accgggatta actgctgcat cgctgtcgtc gcaatcagta ttatttgcaa caccgcaagc
    17761 aaccattgca ccggcaccgt aagtatcacc gtcgaggtcg gcataagtaa ttaatgcgtc
    17821 gttacaatca gtattatctg caacatatcc ggatggagcg gtgcaggcat ttgtagttga
    17881 agctgcatca ccaaaaccgt cgccatcagc atcagcatag aatacggtag taacgccttc
    17941 atcagttaag ccatcgcaat catcatcaac gaggttacaa acttcagtag cacccggatt
    18001 aattgcggca tttgcatcat tacaatcagt attatttgca acataaccta taggagcaga
    18061 acacgctgtt gtagttgaag ccggatcacc ataggtatcg ccgtcggtat cagcgtagaa
    18121 agtgagcgta acaccttcat ctgttgatcc gtcgcaatca tcatccacgc cgttgcaaat
    18181 ttctgtagca cccggattga ttgctgagtt ggcgtcgttg caatcgccac ctgcagttaa
    18241 acctgtataa gcatagccaa cacccggaga agtacaagat gtaacacctg tacctgtata
    18301 atacaagtcg gcatcagcat ctaaatacca agttgttgtc ggattgattg ctgcgttagt
    18361 atcatcgcaa tctgaattat ttaatacata acctgcagga ggtgcaccgc catcgcagtc
    18421 gatagctgtt acggcagggt taccataagt atcaccatca gcgtcggcat aaacagtgat
    18481 attcaaacat gaagtcattt catcaagacc catgtctggg gttgttgctg aacgggagtc
    18541 accatcaaaa tctgttgtaa cagcaacagg agtacctgta ttatcaaccg gagataatgc
    18601 tgctgtgttt atatggagat ttgaagctgc gttaacaaaa tttggatttg tgataataga
    18661 atttgtattg ccaccaaaac cggtttgaat tgcaccaatt gttgtgcgat cagtggcgcc
    18721 gatatttcct aaaattgcag aacctgcatt aaaggtattt gttacataat agtcattgta
    18781 attgatattt gtaaatgccg cacttgttgc tgcagaataa attgcataat tcttttgcgt
    18841 tgtgctagtt ccaacttgtg tgtttgccag aatattgttt ctaatatcta aagcagatgc
    18901 accagaacct atatacaagg cagtagtaag cgctgaagtt gatccgctac ccatgctgcc
    18961 attcatactg atactattat aatacagatt aataccaccg gcagttgtag ttaatgttga
    19021 actaccaccg ataacaccaa tagcaatacc cattgcagat gagttaccga aactactcca
    19081 gttagaaccg ttcactccat atatcatatt attagaaaca gttatgttgc tagaggcatt
    19141 tcctgttcca atggttatac cgcgaccggc ataaccacct gtatttgtag aaataattgt
    19201 tcctaaagta ttgcgactga ttgttgaatt cacaacacca gtttcaatag aaataccgac
    19261 aggagcagtt gttgcagagg tcgtaatatt aattgtgttt tgacttacag aagcacctgt
    19321 tgcctgcaac acctgaatac caataacact agacagcgaa ccgctatagg ttatattatt
    19381 attattgata tcgagattat tgtttaaacc agttgcgcct gcacctacat agatcccggt
    19441 tccaggagag gtgatagcat tgttttgaat aatggtatta tcattatcgt cacctgcagt
    19501 tcccggagtg gcaccacctg ctgcgatgcc ataaccaagt gtagttgaaa cacctgtagt
    19561 aatagtcaga tttttaattg tgttatttgt tgcgccaagc ccggttccca aactcacaag
    19621 cgcgattccg cttggagcag ttgtgttggt attgttaata gtaagatctc ttgaagtgct
    19681 gccattgttt gaaccgtcaa taataatata gtcagcgcca ttcagacgga tcaatgcatt
    19741 ggaagcaagt gaaccactaa tagtagctgt cacgcctgtt gccggtttta tcgtaagtgt
    19801 atttattgca cttgcaccgg tatactgatt aaagacaatt ggataggttt ctgatgcact
    19861 agtatagtca ctttgcaatg caaaagttac ggcacaactt actcctaaac cattaatttt
    19921 tgctgccgcc tgagttaaat tctgatagcc aacaggctga gatgcaccaa tcgcataagt
    19981 tccgcacaaa ggcgcttcag ctgttaacgt cgaactgatc tgatcatttc cggtaaccgc
    20041 atcaccaacc aattcagctt tcgcatatat tgtatatgtg cctgctgcag caagagaagt
    20101 agatgcaaaa gatatattag ttgttactcc gctggcaatt gaagcaatta cctgtgtatt
    20161 attataaacc tctgttaaag tggcatcaac aattctgtaa cgaacagtta cacttgtttg
    20221 cgtagctgta ccattattag taaatgaagc ttgaggggta aaagacactc ctgtaactac
    20281 gcttccaagt gctaccggtg atataaatgc cgttgcctgt aaatcattag taacaggtgc
    20341 agtaaatgcg ccttcatcca aaccaatatc tggtgttgtt gcatttctgg tatctccatc
    20401 atagtcggtg gtaattccgg caattgttgt agcagctgat tcaaggtagc ttgttacagc
    20461 cgtattcaca tgcaagtttg ttgaagaaac aaattgcggg tctttattaa ttgaatttgc
    20521 gttgccacca aatcctgcaa tgattcctgc aagatccgtt ctatcagaag ttaggaaacc
    20581 gaggattgcc gaagcggtat taaatgtatt agaaacaaaa taatcgttat agtttatatt
    20641 actgaatgcc gtatttgcag caacagaata tatcgcataa ttcttttgtg ttgtgcttgt
    20701 tccaacttgt gtatttacaa acacgttatt tcgaatgtcg attgagcttg cgccggagcc
    20761 aacatataat gaggccgtaa gagcagtagt agaaccggat cccatactgc cggtcatatt
    20821 cactgagtta taatacaggt taacgccacc ggtagtagta gtcaatgtag agcttccacc
    20881 aattgttccg atagcaatac ccatggaaga cgagttacca aagctactcc agttagaacc
    20941 attcacacca tagatcatgt tgtttgatat ggtgagtgca ctagttgctg ttccagtacc
    21001 tactgtgata ccgcgaccgc cataaccacc agtgttagtt gccaaaatct gcgtaatcgt
    21061 attcctcgat acacttgaag aaacgaatcc tgtctcaaga gaaataccaa caggcgcagt
    21121 cgaagctgag gtggtaatat tcaacgtatt ctgactaaca gttgcgttta aactgtttgc
    21181 caattgaata ccaataacac ctgaaagtgt tgaattatat gaaatactat tcccgctaat
    21241 tgacaaattg tcattaccac cggaagtcac agatgcagtt ccatttgcat agatgcctac
    21301 cggagcagct gtaatagtat tattgctgat cgtattattg tcattatcag caccagcaga
    21361 tcctggtgtt gagccaccaa ttgaaatacc gtatcctatt gtagtagcga cacctgttga
    21421 gagggtggta ttagtaaccg tattactagt tgctccagca ccagttccaa ggcttaccag
    21481 cgagatcact gttggcgctg tagtagcagt gtttgtaata ttcaacgcat tgcctcctgt
    21541 gttcaatcca ttgattgtaa ctcgatcggc gccattcaat ttaatcaagg ctgcgctaga
    21601 taccgaacca gaaatcgttc tggttgcgcc tccattaggt tgaatggtaa tggtatatgg
    21661 gcttgcaaat tcattcaatc catttgcagc tgtttctgca gtaagatctc catttatatt
    21721 aaggatgatg tttcctgaca ccaatttcgc attgatatca gcaaatgccc caccggcatt
    21781 tgtaagtgaa gtgtagtcgc atccagaagt acaaactgta aatgtgcctg aaatggaact
    21841 aacaatatta aattgattga tggttcctgt aagtgggaaa gctgctgatg ttaaggcaac
    21901 actagttggt gttgcagcaa aaacaccatt attgattcca atatttggtg taccggcatt
    21961 atcctgagca acaacaaaat attgaacagt gtttccaatt gcaattccag cgcctccaaa
    22021 taattttgaa taatcaattg taaaatcaaa cggcgacgta ctaccgttag cctctacata
    22081 tttccatcca tccgttgcgt ttgtattatc attgaaagta ttagcattgg tcgtgagctt
    22141 gtaataaaca cgagggcgcg ttccggatgt accatttaca ccacttaaat ctgtaatagt
    22201 taccgaggag aatgattggg ttccagctac ggcactgttc ggaattgcgg tgtatgcgat
    22261 tgtaggtcca acaaggtcgg cagttatacc cgcaagctcc catgcaccta tatctggtgt
    22321 tgaggcattt cgtgttgttc cagtaaagtc gtcagtaaac ccaactattg cagcaccacc
    22381 actttcaatt tgggtagcaa cagctacact atacttcaag aagtttgcac tggcgcctga
    22441 tgttgattgc caagtaggat tttcagttac agacgccgca tctcttgtag aaacgagtgc
    22501 cttataagca gtcatcgttg attgagcagt agtgccatca taataaatta cattcgttgc
    22561 agatggtgtt cctgcataaa agaggttatt atttgaactt gcgctata
//
LOCUS       DAOQHY010000040        21417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1109_length_21417_cov_6.008098, whole genome shotgun sequence.
ACCESSION   DAOQHY010000040 DAOQHY010000000
VERSION     DAOQHY010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 21417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21417
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1109_length_21417_cov_6.008098"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2067)
                     /locus_tag="PLL28_05035"
     CDS             complement(<1..2067)
                     /locus_tag="PLL28_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF68718.1"
                     /translation="MAKKNEEKKRYKFKEMKVYGSLENFFGNQKNYRIVFDETEVCYV
                     NVELQLFNILFDEEEWSAKVKIKASEYYEAKEMCNIDKDITVTPDQNVIIVREGWGTP
                     NTGWWKKGTYKWEIFIDDVLVGTNYFYITNNGAVKPGFNPYFDINAVRLFNSPKGNTP
                     KAERKYFSAFNKDTAQYISVELDLNNKIYTEKYFPLELNLYFYNDLRQLKGACYWFEH
                     INDSRKNVVMDVGYGAENPGFWFEDKYTCEVVFMDTIVAVIPFEVGTTEVPLTGQQVW
                     LTSSDSSTDAFKEAEVMSDEANYDAAKKELDELIGLESVKKEINELTTYLKFLKLRQE
                     KGFEETNRINLNMVFTGNPGTGKTTVARMLGKIYKSLGLLKRADVTEVGRVDLVAEFI
                     GQTAPKTKAAIDRARGGILFIDEAYALSDRGDDNKDFGKEVIEVLIKEMSDGPGDVAF
                     IFAGYPREMRDFLNSNPGLSSRVSQVISFPDYNPDELMAIADYDSKRRSVMIAEDARN
                     LVYMKVVEAYRNRDNKFGNARFVNSIMEEAKQNMALRLMKSENPEALSKDDLSTIILP
                     DIEEIFGLGNSKKVELPIDEVLLQDALGELKALTGMANVKNEIEETVKLVRYYKEIGR
                     DVKRSFSMHTVFTGNPGTGKTTVARILVKIYKALGILERGQLIETDREGMVAGFVGQT
                     AIKADSM"
     gene            2194..2949
                     /locus_tag="PLL28_05040"
     CDS             2194..2949
                     /locus_tag="PLL28_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HNF68719.1"
                     /translation="MNRIKLSEILTQPPKGMDKSDHEDKTKALIEELGELQNVLFAEG
                     KHSLLVVLQGMDASGKDGVIKEVFSGVNPMGCRVKGFKKPSEEEMKHDFLWRVHKEVP
                     EKGMIQIFNRSHYEDVVIQRVHEWVTLPVIKERYALINAFEKLLQQNGTIILKFYLHV
                     SRDEQQERLKERLSDPRKMWKYNANDLEESKRWDEYMVAYEDAFNTCSPDIPWMVIPA
                     DKNWYKEYLIAKTIVEALKKLDMQYPRIDGQKG"
     gene            3107..4246
                     /locus_tag="PLL28_05045"
     CDS             3107..4246
                     /locus_tag="PLL28_05045"
                     /inference="COORDINATES: protein motif:HMM:NF012282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68720.1"
                     /translation="MIFLSLLLGIIEGKAQIVSDFTSNDESWKVYNEPSGGAPYLPIV
                     WSSTGGNPGGYISETDISIGAWGWLAPSEFKGDLSAYYNFYLSFDLREDSHVGLMDSY
                     YDVMICNASGDTMVFNTTYDPLPINTWVNYIIPLTTAAGWKYGSSIAGGAPAVTAAQM
                     TAILGDVSKMRIRAEFSGLTYETNGMDNVMFTCDLLLPVQLTSFSAEAINNKLSRLFW
                     TTESESNCHGFIIEKSVDFVTFDSIGFIEGNGTTSTPHDYEFYDNNFMTSAYYRLREV
                     NYEGRMFSSDIVYLKNAYSNILTTFVYPNPTDDIITIVADDVNRLFRYTVSDLSGRNM
                     MEVPVGDYNGYFTASISMAQYPSGVYIITMEKKDGVDIQKINLIK"
     gene            4581..5096
                     /locus_tag="PLL28_05050"
     CDS             4581..5096
                     /locus_tag="PLL28_05050"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNF68721.1"
                     /translation="MAYIRGLQSGDTEVLKTVYERYAPALLGVISRMVQDDYLAEDIL
                     QEAFVKIWHNASAYDASKGRLFTWMLNICRNLTIDKMRSRGYKAGKATVMDETRLYED
                     PGKIYDAIRPETIGLREMVDKLKPEWKELIDLIYFKGFTQQEVAEALNMPLGTVKTRL
                     RSAIQELRTIF"
     gene            5100..5894
                     /locus_tag="PLL28_05055"
     CDS             5100..5894
                     /locus_tag="PLL28_05055"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HNF68722.1"
                     /translation="MDTKDYISSGILELYVTGALTEAERLEVEKMARLYREVKQELES
                     IEMAMEDYAFAHEKTPSPRVLEGILNNIEKTENEQVKILPMSGGSRLYSRFRMLAFAA
                     SILLFLSMVTNIYYYSRYQRVSGELADLRDDNTFLTDQYKSLKANYNGMEKEMAMLHD
                     PAMIALTMKGTAMSPASTSIVYWNKGTGDLYLNANNLPKPADGMQYQLWALVEGKPVD
                     AGVFTMDEGIHQMKLIHEADAFAVTLEPMGGSVSPTLQQLYVMGGV"
     gene            6029..6409
                     /locus_tag="PLL28_05060"
     CDS             6029..6409
                     /locus_tag="PLL28_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNF68723.1"
                     /translation="MESQAISTQLTDYAAIFGQFIVAAGFVITAIYFAQKLGPKRGGK
                     IKMSSFECGLDSIGNARLPFSVKYFLVAILFVLFDVEVIFFYPWAVNFRELGESGFVK
                     MLVFMAPLLLGYFYIIKKGALKWE"
     gene            6427..6969
                     /locus_tag="PLL28_05065"
     CDS             6427..6969
                     /locus_tag="PLL28_05065"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HNF68724.1"
                     /translation="MALQKVNEIPEMYTGEGYALTKLEKVVGLARANSIWPLPFATSC
                     CGIEFMATMAATYDLGRFGAERLSFSPRQADLLMVMGTISKKMGPVLKQVYTQMAEPR
                     WVIAVGACASSGGIFDTYSVLQGIDKVIPVDVYVPGCPPPPEAILDGLIKIQELVKIE
                     GFRRRDSPEYKQLLAGYGIE"
     gene            6969..7478
                     /locus_tag="PLL28_05070"
     CDS             6969..7478
                     /locus_tag="PLL28_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNF68725.1"
                     /translation="MENKIENHLVESLLREQFGDAIKASEESYGMLDITVSRDKIVEI
                     LHWLRDHSILQFNFLTSLCGMHFPEVKNAELSVVYHLHSFINNVRIRLHIFFPANDAN
                     APSVIELFPTANWMEREAFDFFGIRFTGHPDLRRILNVEDMQYHPLLKQYPLEDGTRT
                     DKEDKYFGR"
     gene            7753..8970
                     /locus_tag="PLL28_05075"
     CDS             7753..8970
                     /locus_tag="PLL28_05075"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNF68726.1"
                     /translation="MAKNENIINGVVQTEEELKQYNYLNLGPTHPATHGIFQNILKMD
                     GEIIVDAQPTIGYIHRAFEKLAEHRPYYQVTPITDRLNYCSSPINNIGWHMTVEKLIK
                     AEIPKRVDYMRVIIMELARIADHIICNSVIAVDTGALTGFLYVFQWREKIYEIYELIC
                     GTRLTTNIGRIGGFERDFSDDVWRRIQEFVDEFPAALKEFEKMVMRNRIFMDRTIKVG
                     PISGERALAYGFTGPNLRAAGVDYDVRVMSPYSSYQDFDFIIPVGTNGDTYDRFCVRE
                     EEMWQSLHIIKQAMQKMPSGDFKGDVPEFYLPPKQDVYNTMEGLIWHFKIVMGEVHVP
                     KGQVYHSVEGGNGELGFYLVSDGGRQPYRLHFRRPCYIYYQAYPEMIKGAMLSDAIIT
                     MSSMNVIAGELDA"
     gene            9255..9761
                     /gene="nuoE"
                     /locus_tag="PLL28_05080"
     CDS             9255..9761
                     /gene="nuoE"
                     /locus_tag="PLL28_05080"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNF68727.1"
                     /translation="MTEAKHIELSPEAMMEVGRIISHYPADKQKSALLPVLHIAQKEF
                     GGWLSSESMDYVARLLHIHPIEVYEVATFYSMFNTKPVGKVLIEVCQTGPCCLNGAEK
                     IVSYLEKKLQIKVGETTRDGMFTLKTVECLGACGYAPMFQIGETFYEHLTEEKVDNIL
                     LEMRNKNS"
     gene            9797..11143
                     /gene="nuoF"
                     /locus_tag="PLL28_05085"
     CDS             9797..11143
                     /gene="nuoF"
                     /locus_tag="PLL28_05085"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNF68728.1"
                     /translation="MGRKLLLENLDKPEINTIDGYIKYGGYRALEKALKSMTPDEVTE
                     EVKKSGLRGRGGAGFPTGMKWSFLDKSTDKPRYLLCNADESEPGTFKDRLLMEKIPHL
                     LVEGMIVSSYALGCRTSYIYIRGEYMFILRILEKAIAEAYAKGYLGQNILGTGYSLDL
                     HVHPGAGAYICGEETALIESLEGKRGNPRIKPPFPAIAGLYNCPTIVNNVESIAAVVP
                     IVNNGGEDYAKIGIGKSTGTKLISASGNINKPGVYEIELGVSVEEFIYSDEYCGGIAN
                     GLDIKACVPGGSSVPILPKDLLFKQSDGTPRLMTYESLSEGGFATGSMLGSGGFIVFD
                     EKACIVRNTWNFARFYHHESCGQCSPCREGTGWMERVLHNIEYGHGKMEDIDLLWDIQ
                     RKIEGNTICPLGDAAAWPVAAAIRHFRHEFEYHVKYPHRVFDADHVYQPEMKMQTV"
     gene            11208..12158
                     /locus_tag="PLL28_05090"
     CDS             11208..12158
                     /locus_tag="PLL28_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNF68729.1"
                     /translation="MPKVTIDDITIEVPAGTTILEAARQIGVNVPPAMCYYSKLKGSG
                     GKCRVCLVKVTKGSDKDPRPMPKLVASCRTTVMDGMEVRNVTSPEVIEARRGVVEFLL
                     VNHPLDCPICDQAGECHLQDLSYDHGLSKSRYDFERRTFERIDIGDKIQLHMNRCILC
                     YRCTQVAEQLTPERKHGVLYRGEVSEISTFIATAVDNEFSGNMIDVCPVGALTDKTFR
                     FKSRVWFTKPMYAHRNCPTCSGKVALWYQGEEVLRVTSRKDKWGESEGFICNECRFDK
                     KNTSDWTIEGPAPVDRHSVISQNHYELPYELPVNQKKLSN"
     gene            12204..13238
                     /gene="nuoH"
                     /locus_tag="PLL28_05095"
     CDS             12204..13238
                     /gene="nuoH"
                     /locus_tag="PLL28_05095"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNF68730.1"
                     /translation="MDPILIFKLVLVFVLFAVSLGIAAYSTYAERKIAGFMQDRLGPN
                     RAGPFGLLQPLADGVKMFMKEDIIPETSNRFLFIAGPAITMITALMTSAVIPWGPEIT
                     INDVKVPLQVADINIGVLYVLGVVSLGVYGIMIGGWASNNKYSLLGAVRASSQMISYE
                     LAMGMSIIAIVMSSSSLSLGDIVAGQSGWGGMHWNIFIQPLGCLIFIICAFAECNRTP
                     FDLPESETELVGGYHTEYSSMKLGFYLFAEYVNMFISSAMISCLYFGGYNFPGMEAVQ
                     GNMGEIPFALICFVVLFAKILFFVFFFMWIRWTIPRFRFDQLMNLGWKVLFPLAILNV
                     LITGAVIIFF"
     gene            13273..13821
                     /locus_tag="PLL28_05100"
     CDS             13273..13821
                     /locus_tag="PLL28_05100"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HNF68731.1"
                     /translation="MQSLTNRSKVVSNKHLSLAERLYLPAIFGGLRITFKHMFKKKAT
                     ISYPEEQRDHGRLYRGQHVLKRDEQGRENCTACGLCAVACPAEAITMVAGERKKGEEH
                     LYREEKYAIEYNINMLRCIFCGLCEEACPKDAIYLTDRIAESNYQRGQFIFTKDKLVE
                     GIDKSDRIDVSDRRKAVRKGAM"
     gene            13855..14358
                     /locus_tag="PLL28_05105"
     CDS             13855..14358
                     /locus_tag="PLL28_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNF68732.1"
                     /translation="MTQVFFYGLSALAIASSLMMLFQRNAMYSVLYLLVCFFSVAGLY
                     IMLNAQFLAVVHIIVYAGAIMVLFLYVLMFLNLNTPAAFSKSNMLRMAAMLTGGSIFL
                     LLFSAFYRTQLIVMPDPMNENVGSVQTLGKVLFNDYLIPFELTSVLFLSAMVGVVIIN
                     KKDKVNG"
     gene            14351..14683
                     /gene="nuoK"
                     /locus_tag="PLL28_05110"
     CDS             14351..14683
                     /gene="nuoK"
                     /locus_tag="PLL28_05110"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNF68733.1"
                     /translation="MDNTLSQQLQVIPLENYVMLSIILFSIGVIGILMRRNLIVVFLS
                     IELMLNSINLMAASFSAYHNDPAGQAFVFFIMVVAAAEVAVGLAMLVMIYRNVRSIEI
                     DALSRLKW"
     gene            14720..16630
                     /gene="nuoL"
                     /locus_tag="PLL28_05115"
     CDS             14720..16630
                     /gene="nuoL"
                     /locus_tag="PLL28_05115"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNF68734.1"
                     /translation="MSQNALNLITLLIPALPLLGFVIHILFRDKISEKIAGYHATAWI
                     VISFVCSLILFNHVRSEGAIHVQLFEWFSAGDLSIPFAFQVDQLSVIMLLVITGIGSL
                     IHMYSIGYMHGDDGFNRFFSYLNLFIFFMLTLVMANNYVLMFVGWEGVGLCSYLLIGF
                     WFKNPEYNKAANKAFIMNRIGDLAMLLGLFLLYQYAGTLTFDGVSARFDPSSPDYFAP
                     DTHMVTAITMLLFIGACGKSAQIPLYTWLPDAMAGPTPVSALIHAATMVTAGIYMIVR
                     SNLMFSEAELTMQVITVVGIVTSIFAGAIALKQNDIKKVLAYSTVSQLGLMFFAIGVG
                     AYEAAMFHLVTHAFFKALLFLSAGSVIHGMGGEQDIRKMGGLRSYMKVTHWVFGFGVL
                     AIMAVPPFSGFFSKDTILAAAYVKSPVLWVIGLVSGLFTAYYMMRLYAVVFQGQPRMS
                     EHTKAHIHESPSVMTIPLMVLCALSIVGGLMNIPELFGGNKSMMHFLEGVVPIHEHHV
                     AVSTEWMLMAIASVAIFLVIFIAHRKYRINWVVPESDAEMSGAGKLLNHKFYVDELYE
                     MAIVRPMHMLSNFFYKIIDLKVIDGVVNGTAQFTTWCAGQLRLIQTGTVSFYLFMMAL
                     GMGAIVLFIYLM"
     gene            16634..18103
                     /locus_tag="PLL28_05120"
     CDS             16634..18103
                     /locus_tag="PLL28_05120"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNF68735.1"
                     /translation="MLTLLLILLPIISGLIVFVAPRSASKQIAMISALLSLALGIGVY
                     AQFDASIGTQFAIDVPWIDMLGMRFHIGIDGISMLMIMLSNILMPLIILSTSKDNYEN
                     SALLYGLMLIMQGAMNGVFVALDMFLYYIFWELALVPAYFLVLWWGHGNARKITFKFF
                     IYTLFGSLFMLVAIIWLSLQSNDPSTDISSIYNVVIPENVQGYIFFAFMLAYAIKIPV
                     FPFHTWQPDTYTSAPSPATMLLSGVMLKMGIYSIIRWVIPVMPAAVEEYQTTIIVLAS
                     IGVLYASAIAWVQKDLKTLFAYSSIAHVGLITAGILTASASGLQGGMIQMVAHGINAV
                     GLFFVCQILFRAYGNHTISNMGGIRNNAPLFAGLFLVVMLASVALPLTNAFPGEFLLL
                     NSLYQFNPWLCLLAGSGVILGAVYMFNAYKNVMLGEAEGRSKSFVEISGADKLTLVII
                     AGLIFALGLFPQTITGLTEHSVHDIIMQYQSKISGTILR"
     gene            18103..19482
                     /locus_tag="PLL28_05125"
     CDS             18103..19482
                     /locus_tag="PLL28_05125"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNF68736.1"
                     /translation="MTSVIILASAGIISMFVGIFNAKRLALPLVLLACLAALGIVMFR
                     YNGGYDGLLNNMLVFDDYSHGFNIVMILLTMGVLTISRYYYKDNIEHLSDIYALFIFS
                     LIGAILSVSFQNLIMLFIGIEILSIPLYILATSNRRNLFSNEAGLKYFLMGSFASCFL
                     LLGITFIYGTAYSFDMNAIGRFAADNATALPPMFVVGCVLILGAFIFKISAVPFHFWA
                     PDVYQGSPTVITSFMATVVKTAGFGALLRFLNLTIINNPGVWQEIVQVIAILTLVVGN
                     ILALYQQNLKRMLAYSGIANAGYVLVAVASAGEPTTGFVLYYLASYGVASLLAFAVYT
                     IVKDQTGTDTIEGLKGLFAQNKLLTITLALAMLSLSGIPPLAGFFGKYAIFANALSTG
                     MVWPVIIAVLGSLVGVYYYLRVMAVSFSGGEMNKVHVSPGYAFVLLLTAAILVALGVM
                     PDLFTELIA"
     gene            19582..20295
                     /locus_tag="PLL28_05130"
     CDS             19582..20295
                     /locus_tag="PLL28_05130"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HNF68737.1"
                     /translation="MFKRFFFVTVFMIAAGMLLQSFAGQTIRIEGSDVSISDFTTLNV
                     DVPFDVYFTQDAVVSAKVEGLDNIAQTIEFQQKGKELTLKLKEKGGHYGKVKVFLSAP
                     SLENVNVTKSGSFYTLNQIVNSKSFHAAIAGSGNVDIDANAPKVEIGIAGSGNAKLKG
                     SSKRVDVDIAGSGDCRGKDMLADEVSVDIAGSGNAYVFANDTVDVDIAGSGSVYYSGD
                     PEHVTTDIAGSGNVGPDKL"
     gene            complement(20366..>21417)
                     /locus_tag="PLL28_05135"
     CDS             complement(20366..>21417)
                     /locus_tag="PLL28_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNF68738.1"
                     /translation="RDRDMIYPYEEVKQMAELGFLGMMVSPEYGGGGMDTMSYVLAME
                     EISKVDSSCSVIMSVNNSLVCWGLEQFGTEEQKRKYLPDLASGRKIGAFCLSEPEAGS
                     DATSQRTTAEDKGDHYLLNGTKNWITNGGTASIYLVMAQTHVEKGHKGINCLIVEKGM
                     DGFIVGAKEDKLGIRASDTHTLLFNDVKVPKENRIGDDGFGFTFAMKTLSGGRIGIAS
                     QALGIASGAYELALEYAKSRKAFGKAIADHQAIQFKLADMATEIEAARLLIYKAAQLK
                     DAHENYDLASSMAKVFASEVAMKTTVEAVQIHGGYGYVKEYHVERLMRDAKITQIYEG
                     TSEVQRIVIGRSILK"
BASE COUNT         6062 a         4103 c         4727 g         6525 t
ORIGIN      
        1 catgctatct gctttgatgg ctgtttgtcc cacgaaaccg gcaaccattc cttcgcgatc
       61 ggtttcaatt aattgaccac gctcaagaat tcccagcgcc ttatagatct tcacaagaat
      121 acgggcaaca gttgttttac ctgttccggg atttcctgta aagactgtgt gcattgagaa
      181 actgcgcttc acatcgcgcc caatctcttt atagtatcga accagtttaa ctgtttcttc
      241 tatctcattt ttcacatttg ccataccggt cagcgctttt aattctccaa gcgcatcctg
      301 taacaatacc tcgtcgatcg gcagctcaac ttttttagaa ttgccgagtc cgaagatttc
      361 ttcaatatcc ggtaaaatga tggtgctcaa atcatctttg cttaatgcct cagggttttc
      421 tgatttcatc aatcgcaagg ccatattttg tttagcctcc tccatgatag aattgacaaa
      481 gcgagcattt ccgaatttgt tatcgcgatt gcgatacgct tcaacaacct tcatgtaaac
      541 aagattgcgc gcatcttctg caatcatcac tgatcgacgt tttgaatcat aatcggcaat
      601 tgccatcagc tcatcaggat tataatcagg aaaactgatt acctgtgaaa cgcgtgaact
      661 taatcccgga ttgctattta aaaaatcacg catttcacgt ggatatccgg caaaaataaa
      721 tgccacatcg ccggggccat cactcatttc tttaatcagt acttcgataa cttcttttcc
      781 gaaatcttta ttgtcatctc cgcgatcact caaagcataa gcttcatcaa taaataaaat
      841 tccaccacgt gcgcgatcga tagccgcttt tgtttttgga gctgtttggc cgatgaattc
      901 agcaaccaaa tctacgcgtc ctacttctgt cacatcagcg cgtttcaata aacctaatga
      961 tttataaatt tttcccaaca tgcgtgcaac agttgttttt cctgttccgg gattaccggt
     1021 aaataccata ttgagattta tgcgatttgt ttcttcaaat cctttttcct gacgcagctt
     1081 taaaaatttc aaataagtag taagctcatt gatttctttt tttaccgact caagaccaat
     1141 caattcatcc agctcttttt ttgcagcatc gtaatttgct tcgtccgaca ttacctcagc
     1201 ctctttaaat gcgtcggtgc tactgtcgct gcttgtaagc cacacctgct gcccggtcag
     1261 aggcacttct gttgtgccaa cttcaaatgg gattacggca actatggtat ccatgaatac
     1321 cacttcgcag gtgtatttat cttcaaacca aaaaccggga ttttcagcgc cgtagccaac
     1381 atccatgact acatttttgc gactatcatt gatgtgttcg aaccaataac aggcaccttt
     1441 taattgacgg aggtcattgt agaaatataa atttaattcc agggggaaat atttttccgt
     1501 atagatttta ttgttcaaat caagctcaac ggaaatatat tgtgccgtat ctttattgaa
     1561 ggcagaaaaa tattttcgtt cagctttcgg tgtgtttcct tttggcgaat taaataatct
     1621 aacggcatta atatcgaaat aaggattgaa accgggttta acagcaccat tgttagtgat
     1681 ataaaaataa ttagtgccaa caagtacatc gtcaataaat atctcccatt tgtaggttcc
     1741 ctttttccac caacccgtat tcggtgtacc ccatccttcg cgaacaataa ttacattctg
     1801 atcaggggta actgtaatat ccttatcgat attgcacatc tccttcgcct cataatattc
     1861 acttgctttg attttaacct tcgccgacca ttcttcttca tcgaataaaa tattgaataa
     1921 ctgcaattca acatttacat agcaaacttc tgtttcatca aatacaatgc gataattttt
     1981 ctggttaccg aaaaaatttt ccagcgaacc atacactttc atctctttga atttataccg
     2041 ttttttttct tcgtttttct tagccataat gcttttaaat ttggggtaat gggttgtaaa
     2101 aataaacatt ccctaaaagt ttggtcaaca aattttgttg tagttagtta agcccatttt
     2161 tcaatcataa ggtcaaatcc atacatttac gggatgaata ggattaagct gtcagaaatc
     2221 ctgacacaac cacccaaggg catggacaaa agtgaccatg aggataaaac aaaagccctg
     2281 atagaggaat taggtgaatt gcaaaatgta ttgttcgccg aaggcaagca cagcctctta
     2341 gttgtacttc agggtatgga cgccagcggc aaagacgggg tgatcaagga agtgttcagt
     2401 ggtgttaacc ctatgggatg ccgtgttaag ggctttaaga aacccagtga ggaggaaatg
     2461 aaacacgact ttttgtggcg agtgcataaa gaagtgcctg aaaaggggat gattcaaata
     2521 tttaaccgct ctcattacga ggatgttgta attcagcgcg tacatgaatg ggttactttg
     2581 cccgtcataa aggagcggta tgccttgatt aatgccttcg aaaagttgct gcaacaaaat
     2641 ggcaccatca ttctgaaatt ttacctgcat gttagtcgcg acgaacagca ggaacgcctg
     2701 aaggaaaggc tttcagaccc aaggaagatg tggaaataca atgccaatga cctggaagaa
     2761 agtaagcgat gggatgagta catggtagcc tatgaagacg ccttcaacac ctgctctccg
     2821 gacataccct ggatggtgat tccggctgac aaaaattggt ataaagagta tctgatagcc
     2881 aagacgatag tggaggcctt gaaaaaactc gacatgcagt atcctcgaat cgatggtcaa
     2941 aaagggtaga ctccttagat gccgttaggt ttgaggccgt tgcatgaatg aaaatttagt
     3001 gaaaaatttt tggatttaat gtaggagcga tgtattcttg tatgctattt taacgctaaa
     3061 actgctcttc aggttggcat acccgaaaaa acatataggt atttcaatga ttttcctgtc
     3121 ccttttattg gggattattg agggaaaagc gcagatagtc agcgatttta cctccaatga
     3181 cgaaagttgg aaggtataca acgaaccttc aggaggagcc ccttatctcc ctattgtctg
     3241 gtcatctacc ggaggaaatc ccggcggata tatcagtgaa actgacattt ctattggcgc
     3301 ctggggctgg ctcgcaccct ctgaatttaa aggcgatctc tctgcctatt acaattttta
     3361 tttgtcattt gacctacgcg aagactcgca tgttgggttg atggatagct attatgatgt
     3421 gatgatctgt aatgcttccg gagatacaat ggtcttcaat actacctacg atccacttcc
     3481 catcaataca tgggtcaatt acattattcc gttaactacc gctgccggat ggaaatacgg
     3541 ttcgtctatt gccggtggtg ctcctgctgt gacagccgca caaatgactg ctattctggg
     3601 tgatgtcagt aaaatgcgca tcagagctga attctcgggt cttacctatg agaccaacgg
     3661 catggataat gtcatgttta cctgcgacct actattgcct gttcaactga cttccttttc
     3721 tgccgaagcc attaataaca agctttccag gcttttctgg acaactgaaa gtgagtcgaa
     3781 ctgccatgga tttataattg aaaaaagcgt tgattttgta accttcgatt caataggatt
     3841 tattgaaggt aatggcacta cgtctacgcc tcacgactac gagttttacg ataacaactt
     3901 catgacctcg gcttattacc gattgagaga agtgaattat gaaggaagaa tgttctcttc
     3961 tgatattgtg tatcttaaaa atgcatacag taatattcta actacattcg tttaccctaa
     4021 tccaacggat gatatcataa ccattgtagc cgatgatgta aaccgattat tcaggtatac
     4081 cgtttcagat ctgtccggaa gaaatatgat ggaggttccg gttggtgact acaatggata
     4141 ttttaccgca agcatcagta tggcccaata tccttccggc gtgtatatca taaccatgga
     4201 aaagaaggat ggtgttgata ttcagaagat aaatctgata aaataactga ttaagggctc
     4261 ctttcattta ctgtaaattc gccttccggc tagtttgtat ccaattttag ggcggcagtc
     4321 atccgtcttc gtttcacatt gactaaatat aaattgtttg cactaaggca aattaaaagt
     4381 gaagaaattt ttacgtaatc tattgaatgt caatacatta tagtaaaata ttttatccct
     4441 taacccgggc taacctcaga aatttcaaaa attcctcaag aaaatttaaa gcaaataaat
     4501 ccaatggaac atttccctcg tagatttgcc aagttacaga catcagattg acaccgtctt
     4561 cgaacataca agcaaacgaa gtggcgtaca ttcgtggttt acaatcgggc gacaccgagg
     4621 tattgaaaac cgtttatgag cgctacgctc ccgcactgtt gggggttata agtcgcatgg
     4681 tgcaagatga ttatctggca gaggatattc tgcaggaggc ctttgtaaag atctggcaca
     4741 atgcatcagc ttatgatgct tctaaaggga ggttgtttac gtggatgttg aatatttgta
     4801 gaaacctcac aatcgacaaa atgcgaagca ggggctacaa agcaggaaag gcaacggtta
     4861 tggacgaaac ccgtttatat gaagatccgg gtaagatcta tgatgccatc aggccggaaa
     4921 caattgggtt gcgagaaatg gtagataagt tgaaaccgga gtggaaggaa ctcatcgacc
     4981 tgatttattt taaaggattt acccaacaag aggtggcgga agcgctgaat atgcccttgg
     5041 ggacggtaaa aaccagattg agaagtgcta tacaggaatt aagaacgata ttttaacaag
     5101 tggataccaa ggattacata tcatccggaa tactggagct ctacgtaaca ggcgcgctca
     5161 ccgaagctga aaggcttgag gttgagaaaa tggcccgctt gtatcgcgag gtcaaacaag
     5221 agcttgaatc cattgaaatg gcaatggagg actatgcctt tgcacatgaa aaaactcctt
     5281 cgccacgtgt gttggaaggc atcctcaaca acatagaaaa aacagagaac gagcaggtta
     5341 aaatccttcc aatgagcggg ggaagccgct tatatagccg tttccgcatg ctggcctttg
     5401 ccgccagtat attgttgttc ctgtctatgg tcacaaacat ctactactat agtcgctatc
     5461 agcgcgtttc gggtgaactg gctgatctgc gggatgataa taccttcctt accgatcaat
     5521 ataaatcgct gaaagccaat tataacggca tggaaaaaga aatggccatg ctgcacgatc
     5581 cggctatgat agcccttacc atgaagggaa cagccatgag tccggcgtcc acctccatcg
     5641 tatactggaa taagggcacg ggcgacctgt atctcaatgc taacaatttg ccaaagcctg
     5701 cagatggtat gcaatatcaa ctctgggcac ttgtggaagg taaacctgtt gatgcagggg
     5761 tatttaccat ggatgaaggc atacatcaaa tgaagctgat acacgaagca gacgcctttg
     5821 cagttaccct tgagcctatg ggaggcagcg tatctcccac actacagcaa ctctatgtta
     5881 tgggtggtgt ctgaacgttc tgttttctga acagacaatg tcacaaacac ccgaaatttg
     5941 tgctttttta atcatggtat ttccggtacc gttttcctac ctttgccgtg cctaaaacaa
     6001 gtgttttcgg ctgaatgaca ataagcaaat ggagtcacaa gctatttcta ctcaactcac
     6061 agactacgca gctattttcg gacagttcat tgttgctgca ggatttgtga tcactgctat
     6121 ttattttgcc caaaaactgg gtcccaaaag aggtgggaaa atcaaaatgt cgagtttcga
     6181 atgcggactc gattctattg gaaatgcgcg tttgccattt tctgttaaat atttcctcgt
     6241 agcgatccta ttcgtgttat tcgatgttga agtaatattc ttctacccat gggctgttaa
     6301 cttccgtgag ttaggagaat caggatttgt gaagatgttg gtgttcatgg caccgctgct
     6361 tctgggctat ttttatatca ttaaaaaagg tgcccttaaa tgggaataaa accaaccttg
     6421 taattcatgg cgctgcaaaa ggtaaatgag atccccgaaa tgtatacagg tgagggttat
     6481 gcgctcacca agcttgaaaa agtagttgga cttgcacgtg ccaattcaat ttggcctctg
     6541 ccgtttgcta ccagctgctg cggaattgaa ttcatggcaa caatggcggc aacatacgac
     6601 ctcggacgtt ttggtgccga acgtttgagt ttctcacctc gtcaagctga tctcctgatg
     6661 gtgatgggca ctatttcaaa aaaaatgggc cccgtgctga aacaagtata cacacaaatg
     6721 gctgaaccac gttgggtgat cgctgttggt gcttgtgctt ccagcggtgg aattttcgac
     6781 acttattctg ttttgcaagg aatagataaa gtaattcctg tagatgtgta tgtgccgggt
     6841 tgtccccctc ctccggaagc tatcctcgat ggattaataa aaattcaaga actcgtgaaa
     6901 atcgaaggct tccgccgtcg agattcacct gaatataaac aattattagc cggctacgga
     6961 atcgagtaat ggaaaataaa atagaaaatc acctcgtaga aagcctgctg cgcgaacagt
     7021 ttggcgatgc cataaaagca tctgaagaat catacggcat gctcgacatt acggtgtcac
     7081 gagataaaat tgttgaaata ctacactggt tgcgcgatca tagtattctg caatttaatt
     7141 tccttaccag tctttgcggt atgcattttc ctgaagtgaa aaatgctgaa ttatcggttg
     7201 tttatcacct gcacagcttt ataaataatg tgcgcattcg tttacacatt tttttccccg
     7261 caaacgatgc taatgcgcct tcggtaatcg aattatttcc aacagcaaac tggatggaac
     7321 gtgaagcatt cgatttcttt ggtatacgct ttacaggaca tcccgacttg cgtagaattt
     7381 tgaatgtgga agatatgcaa tatcatccct tgttgaaaca atatccgctg gaagatggaa
     7441 cgagaactga taaagaagat aagtactttg gaaggtgaga atggaagtgt gcaggtgtgg
     7501 aagtgtgcag gtgtgcaagt gtgcaagtgt gcaagtgtgg gaatgtggga atgtgggaat
     7561 gtgagaatgt gggagtgtga gaatgtggga gtgtgggaat cccgttgagg agggatgtcg
     7621 ctaagctcca tgtgggaatg tgaggagggg tgaaaatggg gaagggtgag ttaggttaca
     7681 agttaataaa ataaaataag tgagctcggg attaagtctc aatactcaat atccactact
     7741 cactaccata acatggcaaa aaacgaaaat attattaacg gtgtcgtgca gacagaggag
     7801 gaattaaaac agtataatta ccttaacctc ggtcctacac atcctgctac gcatggtatt
     7861 tttcagaata ttctgaaaat ggatggcgag atcattgttg atgcacagcc aaccatcggt
     7921 tacatccatc gcgcatttga aaaacttgcc gaacatcgtc catattatca ggtaacacca
     7981 attaccgacc gattgaatta ttgctcttcg cctatcaata atatcggatg gcatatgacg
     8041 gttgaaaaac ttataaaagc agaaattcca aaacgcgtgg attacatgcg tgtgattatc
     8101 atggaattag cacgcattgc cgatcatatt atttgtaatt cagttatcgc agtagataca
     8161 ggagcactca ccggattttt atatgtattt caatggcgtg aaaaaatcta tgaaatttat
     8221 gaattgattt gcggtacacg ccttaccaca aatatcggtc gcatcggtgg cttcgaacgc
     8281 gatttttctg atgacgtatg gcgaagaata caggagtttg ttgatgaatt ccctgcagca
     8341 cttaaagaat tcgaaaaaat ggtaatgcgc aaccgcattt tcatggatcg tactataaaa
     8401 gtaggaccca tcagcggtga acgagcattg gcatacggat ttacaggtcc aaacctccgc
     8461 gcagccggtg tagattatga tgtgcgtgtg atgtcgccat acagtagtta ccaggatttc
     8521 gattttatta ttcctgtcgg cactaatggc gatacttatg atcgattctg tgtgcgtgaa
     8581 gaagaaatgt ggcagagttt acatatcatc aaacaagcga tgcaaaaaat gccttccgga
     8641 gattttaaag gtgatgtgcc tgaattctac cttcctccga aacaagatgt ttacaatacc
     8701 atggaaggcc ttatctggca ttttaaaatt gtgatgggtg aagtgcatgt gccgaaagga
     8761 caagtgtatc atagtgtgga aggcggaaac ggtgaacttg gattttattt ggtaagcgat
     8821 ggtggtcgtc agccatatcg actccatttc cgtagaccat gttatatata ttatcaagct
     8881 tatcctgaaa tgattaaagg agcgatgttg agtgatgcta ttatcactat gagttcgatg
     8941 aatgtgattg caggggagtt ggatgcttga ttagaggaga gaggagaaag gaaaaaggag
     9001 agaggagaga gggggagatt tggtaggggt gggctgtcgc tgtgtttaat gttggaattt
     9061 gcaaaaaggg tttagaagcg tagggagatt ttagtttggt ttgcaagttt gagagttagc
     9121 aggttggcaa gttaggaggt gggataggtt tcgttaagca gcaaagagtg gagggaaaca
     9181 aacacacaag cacacgaaca catgagtatt gagagaattg attttagaaa gaagaaaaat
     9241 attaaacagt tataatgact gaagcaaaac atattgaatt atcacctgaa gccatgatgg
     9301 aagtgggtag gattattagt cactatcccg ccgacaagca gaagtctgca ttattgcctg
     9361 tattgcacat tgcgcagaag gaatttggtg gctggctgag ttcggagtct atggattatg
     9421 tggctcgtct gttacatata catcctatcg aagtgtatga ggtggcaacg ttttattcca
     9481 tgttcaatac aaagcccgtt ggcaaggtgc tgattgaagt ttgccaaaca ggaccttgtt
     9541 gtctcaatgg tgcagagaaa attgtatcct atttagaaaa aaaattacag ataaaagttg
     9601 gtgaaactac acgcgacggc atgttcactt taaaaacggt ggaatgttta ggcgcttgcg
     9661 gatatgcacc aatgtttcaa attggagaaa cattttatga gcatttgaca gaagaaaaag
     9721 tagataatat tcttttggaa atgcgcaaca aaaacagtta aacatagtat tagctcattc
     9781 gctaattaaa aatatagtgg gacgaaaatt attattggaa aatttggata agccggagat
     9841 caacacgatt gatggttata tcaaatatgg cggatatcgt gccttggaaa aggcattgaa
     9901 atcgatgacc cccgatgagg ttactgaaga agtaaaaaaa tcaggacttc gaggtcgcgg
     9961 cggcgcagga ttccctaccg gaatgaaatg gtcgttcctg gataaatcta ccgacaagcc
    10021 gcgctacctt ttgtgcaatg ccgatgaatc tgagccgggt actttcaaag atcgcttgct
    10081 catggaaaaa ataccacacc tgttggtgga aggtatgatt gtttcaagct acgctttggg
    10141 ttgtcgcact tcatatattt atatccgcgg agaatatatg ttcatcctcc gcattcttga
    10201 aaaggcaatt gcggaagcgt atgctaaagg atacctcgga caaaatattt taggaaccgg
    10261 ttattctctc gatttgcatg tgcatccggg agcaggcgcc tatatatgtg gtgaagaaac
    10321 tgcgttgatt gaatcattgg aaggaaagcg tggtaatccg cgcattaaac cgccattccc
    10381 tgccatcgcc ggattatata attgtccaac cattgtaaat aatgttgagt caattgcagc
    10441 agttgtgccc atcgtaaata atggtggtga agattatgct aaaattggaa ttggaaaatc
    10501 tacaggaaca aaattgattt ctgcatcagg taatattaat aaacccggtg tttatgagat
    10561 tgaacttggc gtatcagtag aagaatttat ttatagcgat gaatattgcg gaggaattgc
    10621 caacggtctc gacattaaag catgcgttcc cggaggttct tcagtgccta ttcttccaaa
    10681 agatttgtta tttaaacaga gcgatggcac tcctcgatta atgacttatg aaagtttaag
    10741 cgaaggtgga tttgcaacag gctcaatgtt aggttcaggc ggatttattg ttttcgatga
    10801 gaaagcgtgt atcgttcgca atacctggaa tttcgcgcgc ttttatcatc atgaatcttg
    10861 cggacaatgt tcaccatgcc gcgaaggaac aggatggatg gaacgcgtat tacataatat
    10921 tgaatacggt cacggaaaaa tggaagacat tgacttgctg tgggatatcc aacgcaagat
    10981 tgaaggaaat accatttgtc cgctcggtga tgctgctgca tggcctgtag ctgctgccat
    11041 cagacatttc cgtcacgagt tcgaatatca cgtgaaatat ccgcatcgtg tttttgatgc
    11101 tgatcatgtg tatcaaccgg aaatgaaaat gcaaacagta taatattgaa tgaaattaat
    11161 ttaagaaaat agttttgaga taatttgcag ataaccatat aataaaaatg ccaaaggtta
    11221 caatagacga tatcaccatt gaagttcctg ccggaacgac aatattggaa gcagcacgtc
    11281 agatcggtgt gaatgtgcca ccggcaatgt gctattattc caaattaaaa ggtagcggtg
    11341 gtaaatgccg agtttgtttg gtgaaggtta cgaaaggatc agataaagat cctcgtccta
    11401 tgccaaagct ggtagcaagc tgccgcacaa ccgtaatgga tggtatggaa gtgcgcaatg
    11461 ttacttctcc tgaagtgatt gaagctcgtc gcggagttgt tgaattcttg cttgtaaatc
    11521 atccactcga ttgtcctatt tgtgatcagg cgggtgaatg tcatttgcaa gacttaagct
    11581 acgatcacgg cttgtcaaaa agcagatatg attttgaacg ccgtacattc gaacgcatcg
    11641 acatcggcga taaaatacaa ttgcatatga acagatgcat cttgtgttat cgctgtacac
    11701 aagttgcaga acaacttacc ccggaacgta aacacggtgt attatatcgc ggtgaagtat
    11761 ccgaaatctc tacatttatt gcaactgcag tagacaatga attctcggga aatatgatag
    11821 atgtttgtcc tgttggtgcg cttaccgata agacattccg gtttaaatcg cgtgtatggt
    11881 tcactaaacc aatgtatgca catcgcaatt gcccgacatg cagcggtaaa gttgctttgt
    11941 ggtatcaggg agaagaagtg cttcgcgtaa ccagcagaaa agataaatgg ggagaaagtg
    12001 aaggcttcat ttgtaacgaa tgcagattcg ataaaaagaa tacttccgac tggacaatcg
    12061 aaggtcctgc accggtagat cgtcattcgg tgatttcaca aaatcattac gagctgcctt
    12121 atgaattacc tgttaatcaa aaaaaattga gtaactgata cctatatatt agaataagag
    12181 gaatcgaaaa atattaaaag aaaatggatc caattctcat atttaaatta gttcttgtgt
    12241 ttgtgctctt tgcagtatcg ctcggtattg cagcatatag tacatatgcc gaacgcaaga
    12301 tcgcgggttt tatgcaggat cgcctgggtc caaacagagc aggtccattt ggtttattgc
    12361 aaccattggc ggatggtgtg aaaatgttca tgaaagaaga tatcattcct gaaacatcaa
    12421 acagattttt atttattgcc ggtcctgcca tcaccatgat tactgcatta atgacgagcg
    12481 ccgttattcc atggggcccg gaaattacca ttaacgatgt gaaagtgcca ttgcaggttg
    12541 ccgatattaa tattggtgtg ttgtatgtat taggggtagt gagtcttggt gtttatggca
    12601 tcatgattgg cggatgggct tcgaataata aatattcttt actcggcgct gttcgcgcat
    12661 ctagccaaat gataagttat gaactggcaa tgggcatgag tattatagca attgtcatga
    12721 gcagcagttc tctcagcctt ggtgatatcg tggccggaca aagtggttgg ggtggtatgc
    12781 actggaatat ttttattcag ccattaggtt gtttaatatt tattatttgt gcatttgccg
    12841 aatgtaacag aacaccattc gaccttccgg aatctgaaac agaattggtt ggcggatatc
    12901 acacggaata tagttcaatg aaattgggat tttatctgtt cgcagaatat gtgaacatgt
    12961 ttatctcaag tgccatgatt tcttgtcttt atttcggcgg atataatttt cccggaatgg
    13021 aagcggtgca agggaatatg ggagaaatac catttgcatt aatttgtttt gttgtgctat
    13081 ttgccaagat cctctttttt gtattctttt ttatgtggat ccgctggacc atcccgagat
    13141 tccgttttga tcagttaatg aacctcggat ggaaagtatt attcccgttg gcaatattaa
    13201 atgtattgat caccggagct gtaattatat ttttttaatt gaatagtgta gaccgaaaag
    13261 cagaaaaata acatgcagag tttaaccaac agaagtaaag tcgtttcaaa caagcatctt
    13321 tcgcttgcag aacgcttata tctgccggca atattcggtg gactgcggat tacctttaaa
    13381 catatgttta agaagaaggc gactatcagt taccctgaag agcagcgtga tcatggtaga
    13441 ttatatcgcg gtcagcacgt tttgaaacgc gacgagcaag ggcgtgaaaa ctgtactgca
    13501 tgcggcttgt gtgctgttgc atgtccggcc gaagcgataa caatggttgc cggagaaagg
    13561 aaaaaaggtg aagaacatct gtatcgcgaa gaaaagtatg ccatagagta taatattaat
    13621 atgctgcgtt gcattttttg cggattatgt gaagaagctt gccctaaaga tgcaatctat
    13681 cttactgatc gtattgcaga aagtaattat cagcgtggac aatttatttt cactaaagac
    13741 aaattagtgg aaggaataga taaaagtgat cgcatcgatg taagcgaccg taggaaagcg
    13801 gtgcgcaaag gtgcgatgta aattattaga atcagtaaaa cggaacacat tttgatgacc
    13861 caggtatttt tttacggctt atcggcgctt gccattgcaa gtagtctgat gatgctgttt
    13921 cagcgcaacg ccatgtatag cgtgttgtat cttttggtct gctttttctc agttgcagga
    13981 ttatatatca tgctcaatgc gcagtttctg gcggtggtac atatcattgt ttatgccgga
    14041 gccatcatgg tattgttcct ttatgtgtta atgttcctta atctgaacac gcctgcggca
    14101 ttttcgaaga gtaatatgtt gcggatggca gcgatgctta caggtggatc aatattttta
    14161 ttgttgttct ctgcttttta tcgcacacaa ctgattgtaa tgcccgatcc aatgaatgaa
    14221 aatgtgggca gtgtacaaac gctcggtaag gtattattta acgattacct catcccattc
    14281 gaacttacga gtgtattatt cctctcggct atggtgggtg tagtgattat taacaaaaaa
    14341 gataaagtaa atggataata cactcagtca acaattgcag gtgatcccac tggagaacta
    14401 tgtgatgctg tcgatcatac tattcagcat tggtgtgatc ggtatcctga tgcgcagaaa
    14461 cctaattgta gtttttttga gcattgaact gatgttgaac tctattaacc tgatggctgc
    14521 ttcattttcg gcatatcata acgatcccgc cggacaggca tttgtatttt tcatcatggt
    14581 tgtggctgcg gcagaagttg ctgtaggact ggccatgttg gtgatgattt atcgaaatgt
    14641 acgcagtatc gaaatagacg cattgagccg cctgaaatgg taacgaaata aataatatag
    14701 taaacgacaa tattttcacg tgagtcagaa cgcattaaac ttaattaccc tccttatccc
    14761 cgcgcttcct ttgctgggat ttgtgataca tattttattc agagataaaa tttctgaaaa
    14821 aattgcaggc taccacgcta cagcatggat agtgatttca tttgtgtgct cacttatcct
    14881 gttcaaccat gttcgttctg aaggcgctat tcatgtgcag ctgtttgaat ggttttcggc
    14941 aggtgacctc agcataccat ttgcatttca ggtagatcag ttgagtgtta tcatgttact
    15001 ggtgatcaca ggtattggca gcctcatcca tatgtattcc attggatata tgcatggtga
    15061 tgatggattc aatcgctttt tcagctatct gaatctcttt atctttttta tgctcacgct
    15121 ggtaatggca aacaactatg tgttgatgtt tgtgggatgg gaaggtgtag gcttgtgttc
    15181 atatttactg attggtttct ggtttaaaaa tcctgaatat aataaagctg ccaacaaagc
    15241 attcattatg aatcgcatcg gcgatctggc aatgctatta ggtttatttt tattgtatca
    15301 gtatgccggc acactcactt ttgatggtgt aagtgctcgc ttcgatcctt cctcacccga
    15361 ttattttgct ccggataccc atatggttac ggcaattacc atgttgctgt ttatcggtgc
    15421 atgcggtaaa agtgcgcaga taccattata tacctggtta cctgatgcca tggcaggtcc
    15481 tacgcctgtt tcggcactca tccatgctgc aacaatggtt actgcaggta tttatatgat
    15541 tgtgcgcagt aatttaatgt tcagtgaagc agaacttacc atgcaggtga ttactgtagt
    15601 tggtattgtt acgtcgattt ttgcaggagc aattgcattg aaacaaaacg acattaaaaa
    15661 agtactcgct tattccacag tcagccaatt gggtttgatg ttttttgcga ttggtgtcgg
    15721 cgcatatgaa gcagctatgt ttcaccttgt aacacacgct ttctttaaag cattattatt
    15781 cttgtctgcc ggttcagtta ttcacggaat gggtggtgaa caggatatac gaaaaatggg
    15841 cggactgcgc agttacatga aggtaaccca ttgggtgttc ggtttcggtg ttttagcaat
    15901 tatggcagtg ccacccttta gtggattctt ttctaaggat accatacttg ctgcagccta
    15961 tgtgaagtca cctgtattat gggtgatagg tttagtttcc ggcttattta ctgcgtatta
    16021 tatgatgcgc ttgtatgcag tggtatttca aggccaaccg cgcatgtcgg agcatactaa
    16081 agcacatatc catgaatctc caagtgtgat gacaatcccg ttgatggtgc tttgcgcatt
    16141 gtcaattgtg ggtggtttaa tgaatattcc cgagttattt ggaggcaata aatcaatgat
    16201 gcatttcctt gaaggagtag tgccaataca tgaacatcat gttgctgttt ctacggaatg
    16261 gatgctgatg gctattgcca gcgttgccat cttcctggtg atatttattg cgcatcgcaa
    16321 gtatcgtatt aattgggttg taccggaaag tgatgcagaa atgagcggcg ccggaaaatt
    16381 actcaatcat aaattttatg ttgatgaatt gtatgagatg gccatagtaa gacctatgca
    16441 tatgctcagc aatttctttt ataagatcat agacctgaag gtaattgatg gcgttgtgaa
    16501 cggcactgca caatttacaa cctggtgcgc cggtcaattg cgattgatac aaacaggaac
    16561 ggtgagcttt tatttattca tgatggctct cggtatgggg gctatcgttt tattcattta
    16621 tttgatgtaa cggatgttga ccttattact catattactg ccaatcatct cgggactgat
    16681 tgtttttgtt gcacctagat ctgcatcaaa acaaatcgcc atgatttcgg ctcttttgtc
    16741 gctggctttg ggaatcggtg tttatgcgca atttgacgcc tccatcggca cacagttcgc
    16801 tattgatgta ccttggattg atatgctggg gatgcgtttc catataggaa tcgatggcat
    16861 cagcatgctt atgatcatgt tgtcaaatat tttaatgcct ctgatcatct tatcaacttc
    16921 gaaagataat tatgaaaata gcgcattatt atacggtcta atgctgatca tgcaaggagc
    16981 aatgaatggc gtttttgttg ctttggatat gttcttgtat tacatattct gggaacttgc
    17041 attggtgccc gcttattttt tggtgttatg gtgggggcat ggaaatgcaa gaaaaatcac
    17101 ttttaaattt ttcatttata ccttgtttgg aagtttgttt atgctggtgg cgattatctg
    17161 gcttagcctc caaagtaatg atccttcaac cgacatttca tcaatttata atgttgttat
    17221 acctgagaat gttcagggat atatcttttt tgctttcatg ctggcgtatg ccattaaaat
    17281 tccggtattc ccgttccata cctggcaacc ggatacttat acaagtgcac catcgcccgc
    17341 aacaatgttg ctttccggtg tgatgttaaa aatgggtatt tatagtatca tacgctgggt
    17401 gatacctgtt atgcctgcag cagtggaaga atatcaaact acaattattg ttcttgcatc
    17461 aataggtgta ttgtatgcaa gtgctattgc atgggtgcaa aaagatttga aaacattatt
    17521 tgcctattca tcaattgctc acgttggtct cattaccgcc ggaattctta ctgcaagcgc
    17581 atcaggtctt caaggaggta tgatacaaat ggtggcgcat ggtatcaatg ctgttggttt
    17641 attctttgtt tgtcagatat tattccgtgc ttatggcaac cataccatta gcaatatggg
    17701 cggaattaga aataatgcac cgctatttgc aggattgttt ttggtggtaa tgctggcatc
    17761 cgttgcattg ccgcttacaa atgcgttccc tggcgaattc ttattgctca attctttata
    17821 tcaattcaat ccctggctct gcctgttagc aggaagcggt gtgattttag gcgcagttta
    17881 catgttcaat gcatataaaa atgtgatgct tggagaagct gaaggtagaa gtaaatcatt
    17941 cgttgaaatt tccggtgccg ataaacttac tttagtgatc atcgccggat taatattcgc
    18001 actcggatta ttccctcaga caattacagg gcttaccgaa cattcagtgc atgatattat
    18061 catgcaatat caatctaaga tcagtggaac aattttaaga taatgacatc agtaatcatt
    18121 ttagcatctg caggtatcat ctccatgttt gtggggatat tcaacgcaaa acgtttggcg
    18181 cttccgcttg tattgcttgc atgtttggca gcattgggca tcgtgatgtt cagatataat
    18241 ggcggttatg acggactgct caacaacatg ctggtgttcg acgactattc gcatggattt
    18301 aatattgtta tgatattact taccatgggt gtgctaacta tcagcagata ttattataaa
    18361 gacaatatcg aacacctttc agatatctat gccttattta ttttttcact gatcggtgca
    18421 atactttcgg tttcatttca aaacctcatc atgctgttta tcggtattga gattttaagt
    18481 ataccattgt atattctcgc taccagcaat cgccgcaatt tatttagcaa tgaagcaggg
    18541 ttgaaatatt tccttatggg ttcctttgct tcttgtttct tattactggg aattactttt
    18601 atatatggaa ctgcctacag tttcgatatg aatgcgatag gcagatttgc tgccgataat
    18661 gctactgcat tgcctccaat gtttgtggta ggatgtgtgt tgattttagg cgctttcatt
    18721 ttcaaaatat cagcagtacc atttcatttt tgggcaccgg atgtatatca gggttctcca
    18781 actgtaatca cttcatttat ggcaactgtt gtgaaaactg cgggatttgg tgcgttgcta
    18841 agatttttga acctgactat cattaataat ccgggcgtgt ggcaggagat tgtgcaggta
    18901 attgccattt taaccttggt ggtaggaaat attcttgcat tgtatcaaca gaatctgaaa
    18961 cgtatgttgg cttattccgg aattgcaaat gccggatatg tgcttgttgc cgttgcctca
    19021 gccggtgagc cgacaacagg cttcgtgctt tattacctcg cttcttatgg cgttgccagt
    19081 ctgcttgcat ttgcggttta tactattgta aaagatcaaa ccggaactga tacgatagaa
    19141 ggattaaaag gcctcttcgc acaaaataaa ttattgacaa tcaccttggc attggcaatg
    19201 ttgtctctct caggtatccc tccactggcc ggattcttcg ggaaatatgc aatttttgca
    19261 aatgcacttt ctacaggcat ggtttggccg gtgatcatag ctgtgttggg ctctttggtc
    19321 ggcgtgtatt attacctgcg tgtgatggca gtatctttca gtggcggaga aatgaataag
    19381 gtgcatgtaa gccccggtta tgcgtttgtt ctattattga cagctgctat cctggtagcg
    19441 ctcggagtaa tgcctgacct ctttactgag ctgatagcct gattaaaacg cgttaaattt
    19501 ccttaaagtg tgggcctcta agtaacaaag gctttgcttc aacagttata gtagttactt
    19561 ttaatcaaca aaataactgc tatgttcaaa cgatttttct ttgtaactgt tttcatgatc
    19621 gcagccggta tgctgttgca atcatttgcc ggacaaacaa tccgcattga aggctctgat
    19681 gtttcaatca gtgatttcac taccctgaat gttgatgtgc cttttgatgt gtacttcacg
    19741 caggatgcag ttgtaagtgc caaagttgaa ggactcgaca atattgcaca aacgatagag
    19801 tttcagcaaa aagggaaaga gttgacactg aaattaaaag agaagggtgg tcactatgga
    19861 aaagtaaagg tttttctgtc ggcaccatca ctcgaaaatg tgaatgttac caagtccggt
    19921 agtttctaca ccttaaatca aatcgttaat tcgaaatcat ttcacgcagc tattgccggt
    19981 tcaggaaatg ttgatataga tgcaaatgca ccaaaagttg aaataggtat tgccggttcc
    20041 ggaaatgcaa aattgaaagg aagcagcaag cgtgttgatg ttgatattgc aggcagtggc
    20101 gattgtcgtg gaaaagatat gctggctgat gaagtttctg tcgacattgc aggctccgga
    20161 aatgcatatg tttttgcaaa tgatactgtt gatgttgata ttgcgggaag tggaagcgtt
    20221 tattattccg gagatcctga gcatgttaca actgatattg ccggaagcgg caatgtcgga
    20281 cctgataaat tataaccgaa aatctatcca ataaaaaaag ggaatcgcaa cacgattccc
    20341 tttttagttt ttatttattg aaatattatt ttaaaatact ccttccgata acaatacgtt
    20401 gcacttcact tgtgccttca tagatttgag tgattttagc atcgcgcatc aatcgttcta
    20461 cgtgatattc ttttacgtat ccgtaaccac cgtgaatttg aactgcttca accgtagttt
    20521 tcatggcaac ttctgatgca aatactttcg ccatgctgga tgctaaatcg taattttcat
    20581 gcgcatcttt taattgtgct gctttgtaaa taagtaatcg cgcagcttca atttctgttg
    20641 ccatgtcagc aagtttaaat tgaatggctt gatgatcggc aattgctttt ccaaatgctt
    20701 tgcgtgattt tgcgtattcc aatgcaagct catatgcacc tgatgcaatt cccaatgcct
    20761 gactggcaat accaatacga ccaccactca atgttttcat ggcaaatgta aatccgaatc
    20821 catcatcacc aatgcgattt tctttaggca cttttacatc gttgaacaat aaagtatgtg
    20881 tatcacttgc gcgaataccc aatttatctt ctttagcgcc aacaataaat ccatccatgc
    20941 ctttttctac gataagacag ttaataccct tatgtccttt ctcgacatgc gtttgcgcca
    21001 tcaccaaata aatacttgca gtgccaccat tggtgatcca gttttttgtt ccgttcaata
    21061 aataatgatc acctttatct tctgctgttg tgcgttgcga tgttgcatca ctgcctgctt
    21121 caggttcgct caaacaaaat gcaccgattt ttcttcccga tgccagatcc ggtaaatact
    21181 tgcgcttttg ttcttcggtg ccaaattgtt ccaatcccca acaaaccaat gaattgttta
    21241 cactcataat tacagagcac gaactgtcga ccttagaaat ttcttccata gccaacacat
    21301 aactcatggt gtccataccg ccgcctccgt actccggaga aaccatcatg cccaaaaatc
    21361 cgagttctgc catttgcttc acttcttcat atggataaat catatctctg tcgcggt
//
LOCUS       DAOQHY010000041        20837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1144_length_20837_cov_5.762006, whole genome shotgun sequence.
ACCESSION   DAOQHY010000041 DAOQHY010000000
VERSION     DAOQHY010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 20837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20837
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1144_length_20837_cov_5.762006"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..1769
                     /locus_tag="PLL28_05140"
     CDS             90..1769
                     /locus_tag="PLL28_05140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNF68739.1"
                     /translation="MRRRNFIRNLGMASPAVVFGPSFMEWHKWLPKEAGVGSVLIIGA
                     GAAGLYAARALQDAGVDVTILEASDTHGGRVRPLPGFADFPTEAGAEFVHGKGNVTGT
                     PPSFLWSSINDYNPDLLLEYGSHKEMYDIGGVIKTEPYWDADLETCWQFYLNMYAYTG
                     DEMLMSEYIDTEYGITPAHPYWHFYEAWIGSEFGTSVDRIGMKSIAISELLWLTGGKD
                     YLLDTSYRDLLDTLFFAPILDKVQYNQVVTGIDYFFPGVTVTVAGGATFSADKCIIAV
                     PLPILKNAEIIFNPPLPMQNQTAIDTLDMGAGMKIFLKFSTNFWGSDIGDITVDGYST
                     FLWAPGIDKTGATNNVLICFIMGENAEYMSALDAGAIDVALAELDALFDGAATANYVD
                     GFIQDWSKEPHIKGAYSFPAPNTYTTETDTTRLDLATPVDCKLFFAGEATNNNHPATV
                     HGALESGARAAAEILACGFSTVHTPETAFAQAYAHDNTAFFAITGNTSGTWSLLLTSI
                     DGKTVHTFMENSNIQGAQTFQFPLGNIASGNYMLTAMQNGKVRCSVMINKS"
     gene            complement(1770..3791)
                     /gene="uvrB"
                     /locus_tag="PLL28_05145"
     CDS             complement(1770..3791)
                     /gene="uvrB"
                     /locus_tag="PLL28_05145"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNF68740.1"
                     /translation="MPFELISQYSPTGDQPGAIKQLTEGLLAGEPSQVLLGVTGSGKT
                     FTIANVIQNLQKPTLILSHNKTLVAQLYGEFKQFFPNNAVEYFVSYYDYYQPEAYIAT
                     TDTYIEKDQSINAEVEKLRLKTTTSLQSGRRDVIVVATVSCIYGMGNPAEFKDSIVRA
                     NVGDKMSRNYFLHRLVDALYSRAAVDFKRGNFRVNGDVVDVFPAYADIGYRITFFGDE
                     VEEIETFNPENGKKLERIQNIAIFPANLYIAPKDILPSIITDIYAELDAQVKYFESIG
                     KYIEAKRLSERVNFDIEMIKELGYCTGIENYSRFFDRRTKGQRPFCLIDYFPDDYLLV
                     IDESHATVPQIGAMSGGDRARKMNLVEYGFRLPSALDNRPQSFDEFMTLLNQVIYVSA
                     TPGDFELNQTGGVVVDQVVRPTGLLDPPIEIRPTLNQVDDLLHEIDERIKKDERILVT
                     TLTKRMAEELSKYMLKMGVRCKYIHSEVETLERVEILRDLRIGRIDVVVGVNLLREGL
                     DLPEVSLVAIMDADKEGFLRNTRSLTQTAGRAARNANGMVIMYADKITDSMQRTINET
                     TRRRQRQMEYNAEHGITPKTVIKSTDEIFTIGKDNKKSVQAYIEPQDVSLSLVADPVV
                     HMMGKPQLEKAIADTKKKMLAAAKDMDFLEAARLRDEMYALEKLLDEKS"
     gene            3891..4337
                     /locus_tag="PLL28_05150"
     CDS             3891..4337
                     /locus_tag="PLL28_05150"
                     /inference="COORDINATES: protein motif:HMM:NF037329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HNF68741.1"
                     /translation="MRFREITAITGLGGLYKMESQKASGLIVTSLAEGWTKFISNREH
                     LFSPLENIAIYTSDDTVELLEVMLKAYEQKDSNPPVDAKADNGTLSTYFEKVLPEYDR
                     EKVHISDIKKFIKWFAILDEKGVLKTELEEKAKEAEAEKATDEKSE"
     gene            complement(4390..5229)
                     /locus_tag="PLL28_05155"
     CDS             complement(4390..5229)
                     /locus_tag="PLL28_05155"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68742.1"
                     /translation="MFSARQFPTKLISAAFGVLIIMGVISISFAKNDGAPSGNTGSPG
                     DDQTCAHTMCHSGTASEQIGLISTNVPASGYLAGETYLVTVTVTEADIVKFGFQASPQ
                     NLDGDRMGDMSVIDATETKLTGGGKYITHKSTGTAGSGSRTWTFNWTPDESTGEVGFY
                     VAVNASNNEDDASGDHIYYDAIFIPEDPDNVPVKVDDIATIDCTVNAISNDMIYVQYQ
                     ASGVHAVKAQIFDMMGRELLDEENDTQASSFTLDTSGLSAGCYLVVLTSGTARFVQQI
                     VLP"
     gene            complement(5284..5757)
                     /gene="bcp"
                     /locus_tag="PLL28_05160"
     CDS             complement(5284..5757)
                     /gene="bcp"
                     /locus_tag="PLL28_05160"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HNF68743.1"
                     /translation="MKEIITLQPGDKVPAFSASDQYGNKITAASMKGKWYVLYFYPND
                     DTETCTKEACNLRDNHAALLKAGITVIGVSHAPVESKLKFTKKYNLPFILLADTDLKI
                     CKSFGVYGDKLFMGKVITTIHRITFLINDKGRIEHVISNVKSGQAASQILDLMKK"
     gene            complement(5757..6464)
                     /locus_tag="PLL28_05165"
     CDS             complement(5757..6464)
                     /locus_tag="PLL28_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNF68744.1"
                     /translation="MAEKYRKKKLFKTKDWTEISAQSSWRMFKIMAEFVDGFETMDKI
                     GPCVSIYGSARTKPDHKFYKLSEQIAARLVGEGFGIITGGGPGIMEAGNKGAYKEGGK
                     SVGLNIELPYEQSHNPYIDRDKLINFKYFFVRKVMLVKYAQAFIFMPGGFGTLDELFE
                     ALTLIQTHKIERVPVIMVGKTYWKGLLAWMRETMLHKEHNISEEDLDLFFVSDEPEEI
                     VQHINAFYATSHLRPNF"
     gene            complement(6474..7943)
                     /locus_tag="PLL28_05170"
     CDS             complement(6474..7943)
                     /locus_tag="PLL28_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNF68745.1"
                     /translation="MAGWLFLCVLLAYFGLLFTISWFTGRKATDSAYYLGNKSSPWYA
                     VAFGLIGDSLSGVTFVSVPGSVATQQFGYMQMVLGYLVGYYVIAKVLLPLYYKMNLTS
                     IYTYLGERFNRNAQITGSLYFILSRLIGAAFRLYVSATVLQLFIFDSFGIPFEVTVTA
                     VIVLILLYTYKGGIKTLVWTDTLQSSFLLAGVLLSIVVIAGALHLDAGGIVQTVRDSN
                     YNNIFFWDPMKQNYFFKQFISGALIAIVMTGLDQNMMQKNLSMRTLPEAQKNIMWFSF
                     VLIFINLFFVSLGSLLYAYADSIQLTLPARTDHLFPMLALDHLGVFAAIVFILGISAA
                     TFSSADSVLTTLTTSFCIDVLKWDPNGNESGKHRRQRHLIHVLFAVLLLIVIIVFNAL
                     NNDAVINQVFVAAGYTYGPLLGLFAFGILTKRSLNAKAVLLICLLPPVACYYLNENSV
                     TWFNGYQIGNELLLINGILTFVGLYVISKAPQQNNIQTA"
     gene            complement(8063..8965)
                     /locus_tag="PLL28_05175"
     CDS             complement(8063..8965)
                     /locus_tag="PLL28_05175"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HNF68746.1"
                     /translation="MKVLQLLGLLTLSGSMAAQMSYFEVGVMGGVANYYGDMTHDYVV
                     ADESHFAYGGYIKYNLDHKMGLKFNVYSGTVSAADANSDRANLKARNLSFTSPVTEAA
                     FTFEYNFFGYRPVEFKQRYSPYVYAGVAAFHFNPQAYYEGEWYDLQPLGTEGQGSEYF
                     PYRDKYRLYEFAIPFGVGFKWALTERWNMGLEYGARWTFTDYLDDVSRTYVDRNLLIE
                     SNGIDAYNLSNRSGEYLGGEPFDYGNNDWRGDPTNTDWYMWLGITFSRNLIKGLDEGF
                     RTSAKDQLGCPGPRHINKVKKKKT"
     gene            complement(9092..10009)
                     /locus_tag="PLL28_05180"
     CDS             complement(9092..10009)
                     /locus_tag="PLL28_05180"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HNF68747.1"
                     /translation="MKRALYIVLLSFIPLGTQAQQYLEIGAFGGVSNYQGDLAPVAFV
                     PTETHLSYGAFYKFNLNYWFGLKGSLYSGFISGNDDNSTEEWKQERNLSFVSPVQEAS
                     AVFEVFLKRYNPKYKWNIMSPYIYFGIAIFHFNPMAQYQGVWYDLQPLGTEGQGNPAY
                     PDRKLYPLTQYSYPVGFGIKGSISPEWNVGVEIGYRKTTTDYLDDVSTTYIAKEILLA
                     DNGDIAWQLSNRTDEMNGGFEVLRDDKKYRGDDTNKDWYIFTGVTISRNYEVKGWESR
                     KYKASDNPKKPGLRKSRKKMDCPGVNLKL"
     gene            10185..10901
                     /locus_tag="PLL28_05185"
     CDS             10185..10901
                     /locus_tag="PLL28_05185"
                     /inference="COORDINATES: protein motif:HMM:NF024275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HNF68748.1"
                     /translation="MRSKKVTLTLALFGGSTGADRFYLGQNTAGWLTILLCWLVLPGA
                     VYGIMRYNLYPNWEPFLLARYALPILFHVFATGRYLIMSEESFMSQNVKKSNTFPMVL
                     LSFVITAILIVGSSRLLQTVQIVDIDKAEVQAVLSAETMSQEFRNDEEAYRKEYDNKT
                     LQIEGVVVETGNDFELGTYFALRGKDGDPFGIKCYFLGDHLKEAEQVVMGDKVTMKGI
                     ANGNKLENCTLLERNGEKVK"
     gene            10956..11564
                     /gene="recR"
                     /locus_tag="PLL28_05190"
     CDS             10956..11564
                     /gene="recR"
                     /locus_tag="PLL28_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNF68749.1"
                     /translation="MENAVNEFAKLPGIGKKTALRLVLHMMKQPQDQVAAFGAAMLKL
                     KTDIHYCPKCHNLSDHPGAECSICSGPRRDHHTICVVESIRDLIAIESTNQYHGEYHI
                     LGGVISPIEGMGPEQLFIDDLIAKVNSGTCNEVIMALNPTIEGDTTVYYITKKITKQN
                     VRITSIARGISFGGELEYTDELTLARSLATRRPYDNYMQQSE"
     gene            11561..13570
                     /locus_tag="PLL28_05195"
     CDS             11561..13570
                     /locus_tag="PLL28_05195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF68750.1"
                     /translation="MIKLSIIIVNYNVKYFLEQAVLSAIKACANIPAEIIIVDNNSSD
                     GSVSFIHQKFIDLQNSHTPVHLIANQENTGFSKANNQGIAIAKGEYILLLNPDTVVQE
                     DTFEKCLAYMDAHPKAGGMGVKMIDGKGAFLAESKRAFPSPEVAFYKAFGLANLFPDS
                     KVFGKYHLGYLSENENHEVDVLAGAYMLIRKRVLEEIGYLDETFFMYGEDIDLSYRIV
                     KKGYTNIYFADTSIIHYKGESTKKASFNYVKMFYTAMKIFAQKHFSGPRAGLFVLFLN
                     TAIYFRAGIAALMRMLGKMASPLFDGALLFSGLFLVKLYWEYYVKYIEGGTYPDAYLY
                     INVPAYMLIWLFTLFLSGGYDNNATPGRIVRGMVWGTFIIAAVYGFLPEHLRFSRGMI
                     IAGAALNIALLIGARGFLHFIKYGNIRFGEAPDKRILLVGGVKEITRAQQLLNTLQLG
                     NQLIGFAGNNKNIEHPLLLGNIDELPLIIRNFRINEVIFCADDVPATEIVRLMTLCGK
                     LTDFKIIPPGSDSIIGSNSKNTAGDIYTMEVQMNIHHPQKQRSKRLLDILIATFGIVL
                     SPLLIWFMDNKAGFLNNCFQVLWGRRSWVGYISSSSGISSNGQKLPTLLPGVLHPLSA
                     GTTVTIAPHIAERVNFMYARDYTPAADLMIIKGAWRRLGGMETVM"
     gene            13633..15648
                     /locus_tag="PLL28_05200"
     CDS             13633..15648
                     /locus_tag="PLL28_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNF68751.1"
                     /translation="MKKLSTLLCALVLGTVLLRAQAPTQPNFVLIVADDLNDYVQGFS
                     GHPQAKTPNINKIEKKGTTFTSTYCAGPKCAPSRTSMITGKDTYYTKVYNNDDIGCKN
                     FRKNFREEKGNEVVFTIPELLKDSGGYFTYTLSKVMHCYPGLPDYDTSNVAMCDKGLS
                     WNKAFSYGQGGDMTDVLDYGDEHDDGVTGFNFAVIPDTMEDNTVDYIITDSAISFIED
                     YASNPATYCNKPFFLAIGYKRPHGPFYVPEKYFLPYYNTDYYSVPYDKPYNDPVGATP
                     YNGVVMPPQPDPMWSDYTSLPDDLVGKAMNEEDYVHPDFLEFGDYMMGLIPPPEIAAG
                     LSDSEKTFILQESERANGIIAYLAAIKFFDAQVGRFYDEIKSHPEIFNNTVFIITSDH
                     GYSLGEKTHWRKGCLWETDVRIPMVIADMRTPVKKTCKRVVGNIDLMPTILEMAGVDA
                     PTFPDGSPYMDGVSLTPLLANPNTPWDKPVVSALQLHGYAEGFCFPQYSVRDEEYHYI
                     RYQTNGATAPACNEALSVFQEELYRIGKNKNIDPYEWNNLADNPAYDDVKAHLASFLP
                     GGVNYLQFDRTASADDAAMAVQAFGVFPNPTSEYANFRMEGFTPGAATLQITDLMGRI
                     IRNDEVTVDENGYLEVSYNVTTIQQGYYIGRVIQGNDSMQTEFIVAR"
     gene            complement(15820..17244)
                     /locus_tag="PLL28_05205"
     CDS             complement(15820..17244)
                     /locus_tag="PLL28_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68752.1"
                     /translation="MIQMLKHFSTKELTRFGEYLESPFFNKDEQLSVFYQYVKKYAPD
                     FESRQIEKDRILQKGIPGIDINEKKLGYLMSDIVEHIEGFIRYNQHMADDMEGYVHLL
                     NMYNSWEADKFFEQTLREARTTLDADPFRNAPYFYKAFLLQSELNTFFDRQKKRAYDA
                     SLQEAANYLDLFYLSTKLKYSCELINRQKLVASDYTLRLLKEISDHLRDQSYDDYPSI
                     GIYYQILMTFVEGENEVHFEKLKLLLNEHTEKFPHNEARDMYAYAQNYTIRKINAGDK
                     KYLREYFDLSRSALEKGLLTVDGDLSPWTYKNIVVAALRVGEYEWAEQFIKQFKSRLN
                     EKFRSNAYSYNMGALMFYKGQYGEALRLVNQVEFTDIFYALDTRTMQIKIYYQLDEWD
                     PMQSAIEAFKVYLRRNKTLSDNVKQLYNNFLKFIEKLSKLTKRDKPKLQELMERIDET
                     RQVADIGWLQAKVQEKIGGERAAS"
     gene            17465..18910
                     /locus_tag="PLL28_05210"
     CDS             17465..18910
                     /locus_tag="PLL28_05210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68753.1"
                     /translation="MESSFFTLSKKTYGMIRLENPLNSALRAMMALSLVIGLSAAVDA
                     QTTTIAVNDSIKICEGTDVMIDVLLNDIDPDIEILETDIIAGPTSPLIDYDDDALPEG
                     SYNIYIDPTFVGEDYILYEVCGEDDLCSMGVIVIIVAGTDACVWPGDANQDNICNAAD
                     ILPIGLYYGLMGPDRDDDDGSWENTFADEWTDVPGAYYPNPKYSDINGDGIVDGNDTT
                     YLVANYGFISDVYTPYEAVGGADDPAISLGIFSDTIETGTAVTIPIYFGTDLIPASGI
                     YGIAFELSYDATLIDPSSVHVSFYDSWLGTMGDDMFALQHNDEATGVVEVAVTRNNQI
                     ARTGAGYFGQVSFVMEDNIAGKTTGQITSTASFCISKPNAVDGLGTITPVQISCDSVV
                     VIDFSNSIDNTSSRGIQVFPNPASAELTVTLPESEATIHLINALGQEIMTMQANNTQI
                     TIPVESIPAGTYMIQISGAHINHTQQCIIQH"
     gene            18922..>20837
                     /locus_tag="PLL28_05215"
     CDS             18922..>20837
                     /locus_tag="PLL28_05215"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNF68754.1"
                     /translation="MRSWITLFTSLFIFSVAQASNPAADCVAAFEYEVSGATVYFIDG
                     STADPGPILEWFWDFGDGTTSTESNPVHTYAEPGEYDVCLTIHSDGGCFDDKCESNIP
                     VDGGGADCVVTASLVAIDGSSGHFIAEVTPAADIVTYTWNWGDGGPLYSETTTGAASD
                     PWHTYSDPGFYTVCVTITTGAGCTDEYCFEIEIPAAGDCYALYEYTLDGGLVHFYEAA
                     GGGDIVSYSWDFGDGSISTDPNPSHIWAAEGTYLVCLTIETATGCSDTYCHEITIGGG
                     GDCTSDFEFDLDGLTVHYFETAHAGGGGADIVSYYWDFGDGTSSDAPNPIHTYTEEGT
                     YLVCLTIVTNTGCTSTYCHEVHVELPTGDCLASYVVSSIELTPDGYVIHIENTSTSGG
                     DITTTTWYYGDGTIGDSYDAEHVYTESGTYIICLVIATADGCVDEYCTSVVVGEPGCS
                     SFFEFTTDGPAVHYFEGADGGGYDIVSYLWSFGDGTFSDGPNPIHIYTEAGTYEVCLT
                     ITTASGCVSTYCHIVIVEEGGEFCAAGFETDGIEETPDGWMAHFANTSIGSDLYHWTF
                     GDGGVSELPNPSHLFETAGIYAVCLTIGVGGGDCFDNYCEELFVGGDDDCVNAGIIDS
                     TMDCVEVYEPVCGC"
BASE COUNT         6060 a         4456 c         4258 g         6063 t
ORIGIN      
        1 ggcgaggcag gcattcccac atggagtgca gcgacatccc gccatagcgg gattctcaca
       61 ttctcacatt cccacattaa cttcgtctca tgcgcagaag aaattttatc agaaaccttg
      121 gaatggccag tccggcagta gtattcggac cctcatttat ggaatggcac aagtggttgc
      181 cgaaagaagc cggagtgggc agtgtgctga ttatcggggc aggagccgcc ggattatatg
      241 ctgcgcgcgc tttgcaggat gccggtgttg atgttaccat attggaagca tcagacacac
      301 atggcggcag ggtgcgtccc ttaccgggtt ttgccgattt ccctaccgaa gcaggtgccg
      361 aatttgtaca tggaaaagga aacgtgacag gcactccccc atccttttta tggagttcta
      421 tcaatgatta taaccctgat ctgctattag aatatggctc gcataaagag atgtatgata
      481 tcggtggtgt cattaaaact gaaccatatt gggatgcgga tctggaaaca tgttggcagt
      541 tttatctgaa catgtatgcc tataccggtg atgaaatgct gatgagcgaa tatattgata
      601 ctgaatatgg cattacacct gcgcatcctt attggcattt ttatgaagca tggataggct
      661 cagaatttgg aaccagcgta gatcgcatcg gtatgaaaag cattgccatc agtgaattat
      721 tatggctgac aggcggcaaa gattatttgc ttgacacctc ttatcgtgat cttcttgata
      781 ccttgttctt cgcacccata cttgataaag tgcaatacaa tcaggtggtt accggaattg
      841 attacttttt ccccggtgtt acagtaactg ttgcaggtgg cgctacattt tctgcagata
      901 aatgtatcat cgcagtacca ctccccattc ttaagaatgc cgaaataata tttaatcctc
      961 cactgccgat gcaaaatcaa actgccatcg atacattgga tatgggagca ggcatgaaaa
     1021 tatttttaaa attcagtacg aatttctggg gtagcgatat tggtgatatt acagtggatg
     1081 gatattctac ttttttgtgg gccccgggca tcgataaaac tggtgctacc aataatgtgc
     1141 tcatttgctt catcatgggc gaaaacgcag aatacatgag tgccttagat gcaggtgcta
     1201 ttgatgttgc gcttgcagaa ctcgatgcct tgttcgatgg cgccgccact gctaattatg
     1261 tggatggatt tattcaggat tggagcaaag aacctcatat taaaggcgct tattcctttc
     1321 ctgctcccaa cacgtacaca acagaaacgg ataccacacg tctcgacctt gccacacctg
     1381 ttgactgtaa attgttcttt gcaggcgagg caacgaataa taatcaccct gccactgtgc
     1441 atggtgcatt ggaaagcggt gcacgcgcag ctgctgagat attggcatgt ggatttagca
     1501 cagtgcatac gcctgaaacc gcatttgcac aagcgtatgc acacgacaat acagcattct
     1561 ttgccatcac cggaaataca tcaggaacct ggtcgttatt attgacctcc atcgatggaa
     1621 aaaccgtgca tacgttcatg gaaaatagca atattcaagg tgcacagaca tttcaattcc
     1681 cattgggaaa tattgcatca ggaaattaca tgctcaccgc catgcaaaat ggaaaagtgc
     1741 gctgtagtgt aatgatcaat aagtcctgat caggattttt cgtcgagcaa tttttcgagt
     1801 gcgtacattt catcgcgcag tcttgccgct tcaaggaaat ccatatcctt ggctgctgca
     1861 agcattttct tcttggtatc ggcaatagct ttttctaatt gcggcttgcc catcatatgt
     1921 accaccggat ctgcaacaag actcagtgat acatcctgtg gttcaatata tgcctgaact
     1981 gatttcttat tatccttacc tatggtaaat atctcatccg tagatttaat taccgtttta
     2041 ggtgtgatgc catgttcagc attatattcc atttggcgct gcctgcgacg agtagtttca
     2101 ttgatcgtgc gctgcatgct gtcagtaatt ttatcggcat acataatcac cataccattt
     2161 gcatttcttg ctgcgcgacc ggcagtttgt gtaaggcttc gtgtattacg caaaaaccct
     2221 tctttatcgg catccatgat ggcaaccagt gatacttcag gaaggtctaa accttcgcgc
     2281 aataaattca cacctaccac tacatcaatt ctcccaattc tcaaatcacg cagaatttca
     2341 acgcgctcga gtgtctctac ttcgctgtgt atgtatttac aacgcacccc cattttgagc
     2401 atatatttcg aaagctcttc ggccatgcgc ttggtaagtg tggttactaa tatgcgttca
     2461 tcctttttta tacgctcatc tatttcatgc aacaaatcat caacctgatt taatgtaggt
     2521 ctgatttcga ttggaggatc caataaacct gttggtcgca ctacctgatc aactactacc
     2581 ccacctgttt gatttaattc aaaatcgccg ggtgttgcgc taacgtagat cacctgattc
     2641 aataaggtca tgaattcatc aaaactctgc ggtctgttat ccaaagcact tggcaatctg
     2701 aatccatatt ccaccaggtt catttttcgt gcgcggtcgc cacccgacat tgcgcctatt
     2761 tgcgggactg tagcgtgact ttcatcaatt acaagcaagt aatcatccgg aaaataatcg
     2821 atcaggcaaa agggtcgctg acctttggtt ctcctgtcaa aaaatcgcga ataattttcg
     2881 ataccggtgc aatatccgag ttccttgatc atttcgatat caaaattcac gcgctcactc
     2941 aagcgttttg cttcgatgta ttttccgata ctttcaaaat atttcacttg agcatccagc
     3001 tctgcataaa tatcagtgat aatggaaggc aaaatatcct ttggagcgat atacaggtta
     3061 gcagggaata tggcgatatt ttgaatgcgt tccagttttt ttccattctc aggattgaat
     3121 gtttcaatct cttcaacctc atctccaaaa aaagtgatgc gataaccaat atcggcataa
     3181 gctggaaaca catccaccac atcgccattc acgcgaaaat ttcctctttt gaagtctacc
     3241 gccgcgcgac tatataatgc gtctaccagg cgatgcaaaa aataattgcg cgacatttta
     3301 tcgccaacat ttgcacgcac aatgctgtct ttaaattcgg caggattacc cattccataa
     3361 atacagctaa cggtagcaac aacaatcaca tcgcgtcttc cactctgcaa actggtagta
     3421 gttttcaatc gcaacttttc tacttccgca tttatcgatt gatctttttc tatataagta
     3481 tcggtagtgg caatatatgc ctccggctgg tagtagtcgt aataactgac aaaatactcc
     3541 acggcattat tcggaaagaa ctgcttgaat tcaccataca actgtgctac caatgttttg
     3601 ttatgcgata aaatcagcgt aggcttttgc agattctgaa tcacattggc aatggtaaag
     3661 gtcttaccac taccggtaac acccagcaac acctgcgatg gttcaccggc caacaatcct
     3721 tcggtaagtt gtttgatggc accgggttgg tcgcccgtag gggaatattg actgatcagt
     3781 tcaaatggca tatggaaaaa cccgaaaaat acggaaaatg aatggcacct ttattgcaat
     3841 aaacacctta taacaaaaaa cgtttcatta attttgcaca aaattacgat atgcgtttca
     3901 gagagattac cgcaataaca ggacttggcg gtttgtataa aatggagtca caaaaagcat
     3961 caggactcat tgttacttca cttgccgaag gatggacaaa atttatttcc aatcgcgaac
     4021 acctcttctc cccacttgaa aatattgcca tttacacgag tgatgatacc gttgaattac
     4081 tggaagtgat gctcaaagcg tatgagcaaa aagatagcaa tccacctgtc gatgcaaaag
     4141 ccgataatgg cacattgagt acttacttcg aaaaggtatt accggaatat gatcgcgaaa
     4201 aagtacatat cagcgatatt aaaaaattca tcaaatggtt tgccatactc gatgaaaaag
     4261 gtgtattaaa aaccgaatta gaagaaaaag ctaaagaagc agaggcagaa aaagctaccg
     4321 acgaaaaatc ggaataaata ataacttaac agaaaccttg aaaccctgct caaccggcag
     4381 ggttttcttt tatggtaaca cgatctgttg cacaaatctg gcagttccac ttgtcaatac
     4441 taccagatag catccggccg acaaaccgga agtatctaat gtaaagcttg atgcttgtgt
     4501 atcattttct tcatccaaca attctcttcc catcatgtca aatatctgtg ccttaacggc
     4561 atgaacacca cttgcctgat attgaacata aatcatatca ttcgagatgg cattaacagt
     4621 gcaatctata gtagcaatat catccacttt aaccggcaca ttatccgggt cttccggaat
     4681 aaaaatcgca tcataataaa tatgatctcc ggaagcatcg tcttcattat ttgaagcatt
     4741 taccgccaca taaaatccaa cttctccggt actttcatcg ggtgtccagt tgaaagtcca
     4801 ggttctggat cctgagcctg cagttcctgt agatttatgt gtgatgtatt ttcctccacc
     4861 ggtaagttta gtctccgtag catcaattac actcatgtca cccatcctgt ctccatcaag
     4921 gttttgcggt gaagcctgaa atccaaattt cacaatatcg gcttcggtaa cagttacggt
     4981 aaccaaataa gtttctcctg ccagatagcc gctcgcaggc acattggtgc ttatcaatcc
     5041 tatttgttct gaggcagttc ctgaatgaca catcgtatgg gcacaagtct gatcatcacc
     5101 cggagaaccg gtgttgccgg aaggtgcccc atcatttttt gcgaatgaaa tacttatcac
     5161 acccataatg atcaacacgc cgaaagcagc gctgatcaac ttagttggaa actgtcgcgc
     5221 agaaaacata tgcctgaatt atctgtttat acacgaaatt agaaaaacaa attttattga
     5281 gtgtcatttt ttcatcagat cgagaatctg actcgccgcc tgtccggatt taacatttga
     5341 tatcacgtgc tcaatgcgac ctttatcatt gatcagaaaa gtaattctat gaatagtggt
     5401 aatcaccttc cccataaata atttatcgcc ataaactcca aacgatttac atattttcag
     5461 atccgtgtct gcaagtaaaa taaagggcag gttatacttt ttggtgaact tcaatttgct
     5521 ttctaccggt gcatgcgata cacctatcac cgtgattccg gcttttagca aagcagcatg
     5581 attatcgcgc agattgcagg cttcctttgt gcaggtttcc gtgtcatcgt taggatagaa
     5641 atacaacaca taccatttcc ccttcatgga ggctgcggtg attttattgc cgtattgatc
     5701 ggaggcacta aatgcaggga ccttatctcc cggctgtaag gttattattt ccttcatcag
     5761 aaattcgggc gaaggtgtga tgtggcataa aatgcgttga tatgttgcac tatttcttcg
     5821 ggttcatcag aaacaaaaaa cagatcaagg tcctcttcac tgatattatg ttctttgtgc
     5881 aacattgttt cgcgcatcca tgccagcaat cccttccaat aagttttacc taccatgata
     5941 acagggacac gttcaatttt atgtgtttga ataagcgtta atgcttcaaa caattcatcc
     6001 agcgtaccaa atccaccggg catgaaaata aatgcctgag catatttcac aagcattacc
     6061 ttgcgcacaa agaagtattt gaaattgatc agtttatcgc gatcgatata cgggttgtga
     6121 ctctgttcat aaggcaactc gatatttaat cccacagatt taccgccttc cttataagcg
     6181 cctttgttgc ccgcttccat gataccgggt ccaccgccgg ttataatacc aaaaccctct
     6241 cctaccaatc gcgctgcaat ttgctccgag agtttataaa atttatgatc aggctttgtt
     6301 cttgcagagc cataaattga aacacaggga ccaattttat ccatcgtctc aaatccatct
     6361 acaaattcgg ccatgatttt gaacatgcgc cagctcgact gcgcactgat ttcagtccag
     6421 tcttttgttt taaacaattt tttcttgcga tacttttcag ccataaaaat gtattaagcc
     6481 gtttgaatat tattttgctg aggagccttc gaaattacat ataaacccac aaaggtcaaa
     6541 ataccattga tgagcaacaa ctcattacct atctgatatc cattaaacca cgttacagag
     6601 ttctcattga ggtaataaca ggcaacaggc ggcaacaagc atatcagtaa taccgccttc
     6661 gcattgagac tgcgttttgt taaaatgccg aatgcaaata atccaagcaa aggcccatag
     6721 gtatatcccg ctgctacaaa cacctgattt atcacagcat cattatttag tgcattaaat
     6781 acaattatca ctatcagcaa tagcacggca aataacacat gtattaaatg gcgttgacgt
     6841 ctatgcttac cactttcatt gccatttgga tcccatttca gcacatctat acaaaaactg
     6901 gtagttaagg tagtgagcac agaatcagcg ctcgaaaaag tagctgccga tatccccaga
     6961 ataaatacaa ttgccgcaaa tacgcccaga tgatctaagg caagcatggg aaataaatga
     7021 tctgttcttg ccggtaatgt caattggata ctgtcggcat atgcatacaa caatgaacca
     7081 aggcttacaa aaaacagatt aataaatatc agcacaaagc taaaccacat gatatttttt
     7141 tgagcctcag gtaaggtgcg catactgaga tttttctgca tcatattctg atcaagaccg
     7201 gtcattacaa tggcaatcaa tgcgccgctg ataaactgtt tgaagaaata attctgtttc
     7261 atcggatccc agaaaaatat attattataa ttactgtcgc gaacagtttg cacaattcct
     7321 cctgcatcta aatgcaatgc tcccgcaatt actacaattg ataacaacac acctgccagt
     7381 aaaaatgaac tttgcaaggt atctgtccac accaaagttt taatcccacc tttatacgtg
     7441 tataataata tcaatacaat aaccgcagta actgtcacct caaaaggtat accgaatgag
     7501 tcgaaaataa ataattgcaa aacggtagca gatacataca aacgaaatgc tgctccaatt
     7561 aatctcgaca gaataaaata caaggaacct gttatttgtg cattcctgtt aaatcgttca
     7621 cctagataag tgtaaatgga ggtgaggttc attttataat acaagggcaa caatactttt
     7681 gcaatcacat aatatcccac cagataaccc aacaccattt gcatgtaacc gaattgttgt
     7741 gttgctacgc ttccgggaac actaacgaaa gtaaccccac tcaatgaatc accaataaga
     7801 ccgaaagcaa ctgcatacca tggcgaagat ttattaccaa ggtaataggc gctatctgtg
     7861 gcttttctgc cggtaaacca ggatatagta aacaacaatc cgaaataggc taaaagcaca
     7921 caaagaaata accaacctgc cataatgtat tgtccttatc gcctgcaaaa tagcaggttt
     7981 tattgaatca cctttttagg atatgcacaa aaaaaggatg agtgtatgct catccctatt
     8041 tcgttatttc cattcaggaa atttatgtct tcttcttttt cactttgttg atatgacgtg
     8101 gtcccgggca accaagctgg tcttttgccg aagtgcggaa accttcatca agacctttaa
     8161 tcaggttacg gctgaaggta attcccaacc acatatacca atcggtattg gtaggatcgc
     8221 cgcgccagtc gttattgcca tagtcaaatg gttcgccacc aagatattct ccgcttctat
     8281 tgctgaggtt ataagcatcg ataccattag attctatcag gaggttgcgg tctacgtaag
     8341 ttcgactcac atcatctaaa taatctgtaa aggtccaacg agcgccatat tccaaaccca
     8401 tattccatct ttcggtgagt gcccatttaa agccaacacc aaatggaata gcaaattcat
     8461 acaggcggta tttatcgcgg taaggaaaat attcggaccc ctgaccttct gttccaagtg
     8521 gttgaagatc ataccattca ccttcataat atgcctgcgg attaaaatgg aaagcggcaa
     8581 ccccggcata tacatacggt gaatagcgct gcttaaattc tacagggcga tatccgaaaa
     8641 aattatattc aaaagtaaat gcagcttcag ttaccggaga agtaaaactc aaattacggg
     8701 ctttaagatt tgcacgatcg ctattagcat cagcagctga aacggttccg ctgtatacat
     8761 taaacttcaa acccatttta tgatcaaggt tatatttgat ataacctcca taggcaaaat
     8821 gcgattcatc ggcaaccaca taatcatgcg tcatgtcgcc ataataattg gctacaccac
     8881 ccataacacc tacttcaaaa taggacatct gcgcggccat ggagccggac agtgtaagta
     8941 aaccgaggag ttgtaatact ttcatccttt ttgcgtgtag caatctttta acaaaaatag
     9001 caatttagaa cggatataaa agaatcttag cgggtatata acgtttaaat gacacaaaaa
     9061 gtgtttagta tgtcgaaaaa aagcaacagt gtcagagttt gaggttcacc cccgggcaat
     9121 ccattttctt tctggacttg cgcaaaccgg gctttttcgg gttgtcactg gccttgtatt
     9181 ttctcgactc ccatcccttc acttcgtagt ttctcgagat agttacgccg gtaaagatgt
     9241 accagtcttt attggtatca tcgccacgat attttttatc atctcgaagc acctcaaagc
     9301 ctccattcat ttcatccgta cgattcgata gctgccaggc aatatcacca ttatcagcca
     9361 acagtatctc tttggcaatg taggttgtac taacatcatc cagataatcg gtagtggttt
     9421 tacgatatcc tatctcaacc cctacattcc attcgggcga aatagaaccc ttgattccaa
     9481 accctaccgg atatgagtat tgcgtaagcg gatataattt cctgtcggga taggccggat
     9541 tcccctgtcc ttctgtcccc aagggctgca ggtcatacca aaccccttga tactgtgcca
     9601 tagggttgaa atgaaatatc gcgataccaa aatagatgta tggcgacatg atgttccact
     9661 tgtacttagg gttataccgc ttcagaaaga cttcaaacac cgcagatgcc tcttgtaccg
     9721 ggctgacaaa gctgagattt cgctcttgtt tccattcttc ggtggagttg tcgtcattgc
     9781 ccgaaataaa gcctgaatac aaactcccct tcagtccgaa ccagtaatta agattgaatt
     9841 tgtaaaaagc cccgtatgaa agatgcgtct ccgtaggtac aaaagccacc ggtgccaggt
     9901 ctccctggta gttacttacc ccaccgaatg ctccaatctc taggtattgc tgagcttgtg
     9961 tgcctagtgg gataaatgat aacagaacaa tatacagtgc cctcttcatg aacttgaaca
    10021 tagccgcaga aaatgctgcc tacaaatttg cttaatcgcg taagcaaatc ctacttgaaa
    10081 ggtcaaaaat accgcttttt aactatacag cctgatgtca ctttttaaag tattccctgc
    10141 cattataaca attgtctcta tttttgttca gttcaagcaa catcatgaga tcaaaaaagg
    10201 taacacttac acttgcactt tttggcggca gcacgggcgc cgatcgcttc tatttaggtc
    10261 agaataccgc cggatggctt acaatactgc tttgctggct ggtgcttccc ggggctgttt
    10321 atggcatcat gcgctacaac ctatacccca actgggaacc ctttctgttg gccaggtacg
    10381 cccttcccat cctgtttcat gtgtttgcta caggtcggta tttgatcatg tctgaggaat
    10441 ctttcatgtc acaaaacgtc aaaaaaagta atacttttcc gatggttttg ctcagttttg
    10501 tcattacggc cattctcata gtaggcagct cccgactgct tcagacggtt cagatagtgg
    10561 atatcgacaa ggccgaagtg caagcagtgc tatcggcaga aacgatgtca caagagttca
    10621 gaaatgatga agaagcttat cgcaaggaat acgacaataa gaccttgcaa atagagggcg
    10681 tagtggttga aacgggcaac gacttcgaac tgggaaccta ttttgcactc cgcgggaagg
    10741 atggagatcc atttggcatc aagtgttact tccttggcga ccacctcaag gaggcagaac
    10801 aggttgttat gggcgacaag gttaccatga aaggcatcgc caatggcaat aaacttgaaa
    10861 actgcacttt attagaacgc aatggcgaga aagtaaagtg atccgtaact tcacgtcttt
    10921 ataattactg tatagtgaat ctaccctcac aatggatgga aaatgctgtt aatgagtttg
    10981 ccaagcttcc cggcataggc aaaaaaacag cattgcgtct ggtcttacat atgatgaaac
    11041 aacctcagga tcaggtggcc gcttttggtg ccgctatgct gaaactgaaa acagatattc
    11101 attattgtcc caagtgccat aacctcagcg atcatcccgg tgccgaatgc agtatatgct
    11161 ccggtccgcg acgcgatcat cataccattt gtgtggtcga atctatccgc gatctcattg
    11221 cgatcgaaag caccaatcaa tatcatggcg aatatcatat tttgggaggg gtgatatcac
    11281 ctatcgaagg catggggccg gagcaattat ttattgatga ccttattgca aaagtaaata
    11341 gcggcacatg taatgaagtg atcatggcat taaatcctac aatagaaggt gatactacag
    11401 tatactatat caccaaaaag atcacgaaac aaaatgtgcg cataacttcc atagcacgtg
    11461 gcatttcttt tggtggtgaa ttagaatata ccgatgaact tacgcttgcc cgctcattgg
    11521 ctacccgcag gccttatgat aattatatgc agcaaagcga atgatcaaac tcagcatcat
    11581 catagtaaat tataatgtca agtattttct tgaacaagct gtgttgagtg ctattaaagc
    11641 atgtgctaat attccggcag aaattatcat agttgacaat aattcttctg acggcagtgt
    11701 atcatttatt catcaaaaat ttattgacct tcaaaattct cacacccctg ttcaccttat
    11761 cgccaatcag gagaacaccg gtttttcaaa ggcaaacaac caaggtatag ccattgcgaa
    11821 aggcgaatat attttattac tcaatcccga taccgttgtg caggaagaca cttttgaaaa
    11881 atgtcttgca tatatggacg cacatccgaa agcgggtggc atgggggtta aaatgataga
    11941 tgggaaaggc gcatttctgg ctgaaagtaa acgggcattt ccaagtccgg aagtggcgtt
    12001 ctataaggct ttcggactgg caaatctctt tccggattca aaagttttcg gcaaatatca
    12061 cttggggtat ttatctgaaa acgaaaacca tgaagtggac gtgcttgcag gtgcgtatat
    12121 gctcattcgc aagcgtgtgc tggaagaaat cggatacctt gatgaaacat ttttcatgta
    12181 cggcgaggat atcgatctca gttatcgcat cgtgaaaaaa gggtatacga atatttattt
    12241 cgccgacact tccatcattc actataaagg tgagagcact aaaaaagcaa gctttaacta
    12301 tgttaaaatg ttttataccg cgatgaagat tttcgcacaa aaacatttta gcggacccag
    12361 ggcaggatta tttgttttat ttcttaatac cgccatttat ttccgcgcag gaattgcagc
    12421 cctgatgcgt atgcttggaa aaatggcttc cccacttttt gatggcgcat tattattcag
    12481 tggattattt ctggtgaaat tatattggga atattatgta aaatatattg aaggtggcac
    12541 ttatcccgat gcttatttat atatcaatgt accggcgtac atgttgatat ggctttttac
    12601 tttattttta agtggtggtt acgacaacaa tgccactcct ggccgcatcg tgcgcgggat
    12661 ggtatgggga acattcatta ttgctgctgt atatggtttc ctgcccgaac atttgcgctt
    12721 ttctcgcggt atgattatag caggcgctgc attaaacatt gcattgctga tcggcgcaag
    12781 aggttttttg cattttatca aatacggcaa catacgattt ggcgaggcgc ctgataaacg
    12841 catattgctt gttggcggcg tcaaagaaat tacacgtgcg cagcaattgt taaatacact
    12901 tcagctcggt aaccagttaa ttggatttgc aggaaacaat aaaaacattg agcaccctct
    12961 gttgctagga aatattgacg aattaccgct catcatcaga aatttcagaa tcaatgaagt
    13021 aatattttgt gccgatgacg tgcctgctac cgaaatcgta aggcttatga ccttgtgtgg
    13081 aaagcttact gattttaaaa taattcctcc cggaagcgac agtattatcg gcagtaattc
    13141 caaaaacact gcgggtgata tctataccat ggaagtgcag atgaatattc atcatcctca
    13201 gaaacaaaga agcaagcgac tccttgatat attgattgca accttcggga tagtactcag
    13261 tccgctactg atttggttta tggacaataa agccggattc cttaacaatt gctttcaggt
    13321 tttatgggga aggagatcgt gggtgggtta tatatctagc tcttcgggta tttcatcgaa
    13381 cgggcaaaag cttccgacct tgttgccggg cgtattgcac ccgctttctg ccggaactac
    13441 cgtaaccatt gccccgcata ttgccgaaag agtaaacttc atgtatgcca gagattacac
    13501 cccggcagca gacctcatga tcataaaagg agcctggcga cggttgggtg gaatggaaac
    13561 cgtaatgtaa atttgttgtc gcatacaact atttacagat aacctttctc ttactaacac
    13621 acgcatattg caatgaaaaa gctatcaacc ctactctgtg ctctcgtttt gggcaccgtt
    13681 ttactacggg ctcaggcgcc aacacaacca aatttcgtgt tgatcgttgc cgatgacctg
    13741 aatgactatg ttcagggatt cagtggtcac ccgcaggcaa aaacgccaaa catcaacaaa
    13801 attgaaaaaa agggcaccac cttcaccagt acttattgcg caggtccaaa atgcgcaccg
    13861 agccgcacaa gtatgatcac cggtaaagac acctactata ctaaggtgta caacaacgat
    13921 gacatcggct gtaaaaattt ccgaaaaaac ttcagggaag aaaaaggaaa tgaagtagtg
    13981 tttaccattc ctgaattatt aaaggacagc ggaggatatt tcacatatac cttaagtaaa
    14041 gtgatgcatt gttatcctgg tcttcctgat tatgatacat caaatgttgc catgtgcgat
    14101 aaaggcttgt cgtggaacaa agccttctca tacggacaag gtggcgatat gactgatgta
    14161 ttggattatg gcgatgaaca tgatgatggg gtaaccggat tcaactttgc ggtaattcct
    14221 gacaccatgg aagacaatac cgttgattat atcattacag attcagcgat ttctttcatt
    14281 gaagactatg catcaaatcc tgccacttat tgcaacaaac catttttcct ggctatagga
    14341 tataagcgcc cgcacggtcc tttttatgtg cctgaaaaat atttcttgcc ttattataac
    14401 acagattatt attcagttcc ttacgacaag ccatataatg atcctgtagg tgctacgcct
    14461 tacaatggag tggtaatgcc acctcagccc gatccgatgt ggagcgatta taccagctta
    14521 cctgacgatc ttgttggcaa ggccatgaat gaagaagatt acgtgcatcc cgactttttg
    14581 gagtttggcg attacatgat gggattgata cctcctccgg aaattgctgc cggattatct
    14641 gattcagaaa agacttttat tcttcaggaa tctgagcgcg caaatggtat cattgcatac
    14701 cttgctgcca ttaaattctt cgatgcacag gttggtcgtt tttatgatga gataaaatca
    14761 catcctgaaa tatttaataa tactgtattt atcatcacca gcgaccatgg ttattcatta
    14821 ggtgaaaaaa cacactggcg caaaggttgt ttgtgggaaa cggatgtacg cattcctatg
    14881 gtaattgcag atatgcgtac tccggttaaa aaaacatgta aacgtgtagt tggcaatatt
    14941 gatttgatgc ctactattct tgaaatggcc ggcgtagatg cacctacttt tcctgatggt
    15001 tcaccataca tggatggcgt aagcttaaca ccactccttg ccaatccaaa tacaccttgg
    15061 gataaaccgg ttgtatctgc attgcaatta catggttatg ccgaaggatt ttgcttccct
    15121 caatacagcg tgcgcgatga agaatatcac tacatccgtt atcaaacaaa tggtgctacc
    15181 gctcctgctt gtaacgaagc attaagtgtt tttcaggaag aattatatcg catcggaaaa
    15241 aataaaaaca tcgacccata cgaatggaat aacctggcag ataaccctgc ttacgatgat
    15301 gtgaaagcac atctggctag tttcctaccg ggtggagtaa attacttgca attcgatcgc
    15361 accgcttccg cagatgatgc tgctatggct gttcaggcat ttggcgtttt tccaaatcca
    15421 acaagtgaat atgccaattt ccgtatggaa ggatttacac ccggtgcagc aactttacag
    15481 atcactgatt tgatgggacg catcatccgt aacgatgagg ttactgttga tgaaaatggt
    15541 tacttggaag tatcatataa tgtaacaact atccaacaag gatattacat tggtagagtt
    15601 attcaaggta atgacagcat gcaaacagaa tttatcgttg cacgataatt tcagcaatta
    15661 aataaaacaa aagacccgga tcgagttttc atccgggtct ttttatttat agtaaattat
    15721 gaattgcggt tcggcaaaaa ttaaatagga taagaatcga ggggaatttt ttaattagtc
    15781 aataattcta acgattcaac ttcgtaaaga aagcagactt tatgatgcag cgcgttcacc
    15841 gccaattttt tcctgtactt tggcctgaag ccagccaata tcagccacct gtcttgtttc
    15901 atcaatacgc tccatcaatt cctgcagttt cggcttgtcg cgcttagtga gtttcgacag
    15961 cttttcaata aatttcagga agttgttata aagttgcttt acgttgtcgg agagtgtttt
    16021 gtttctacgg agatatactt taaatgcctc gatagcgctt tgcataggat cccattcatc
    16081 gagttgataa tatattttta tctgcatggt gcgtgtatcg agcgcataaa aaatatcggt
    16141 gaattcaacc tgatttacca ggcgcaaagc ttcaccatat tgtcccttgt aaaacatcaa
    16201 ggcacccata ttgtagctgt aggcgttaga gcggaatttt tcgttgaggc gcgatttgaa
    16261 ttgcttgata aattgctcgg cccattcata ttctcctaca cgcaaagcag ccactacaat
    16321 gtttttatac gtccatggtg aaagatcccc gtcaacagtc agcaatcctt tttcgagtgc
    16381 ggagcgactg agatcaaaat actcacgcaa atatttctta tcgcctgcat ttattttccg
    16441 tatggtgtag ttctgggcat aggcatacat atcgcgcgct tcgttgtgcg gaaatttttc
    16501 ggtatgctcg ttcagcaaca atttgagttt ttcgaaatgc acttcatttt caccttcaac
    16561 gaatgtcatc aatatctgat agtaaatacc aattgacgga taatcatcat aagactgatc
    16621 ccgcaggtga tctgaaattt cttttaacaa acgcaaggta taatccgacg ccactaattt
    16681 ctgacggttg atgagctcgc aggaatattt aagttttgtg gataaataaa acagatcgag
    16741 gtagtttgct gcttcctgaa gactggcgtc ataggctctt tttttctgcc tgtcgaaaaa
    16801 agtatttaat tcactttgta ataaaaatgc tttgtagaaa tacggtgcat ttcgaaacgg
    16861 atcagcatcc agggttgttc ttgcttctcg taacgtctgc tcaaaaaatt tatcagcttc
    16921 ccagctgtta tacatattca ataaatgcac atagccctcc atgtcgtctg ccatgtgctg
    16981 attatatctg atgaagcctt caatatgctc cacaatatcg ctcatcagat aacccagttt
    17041 cttctcattg atatcaatgc cgggaatacc cttctgtaag atgcggtctt tttctatttg
    17101 tcgcgattca aaatccgggg cgtacttctt tacatactga taaaacacac tcaattgctc
    17161 atccttattg aagaatggcg attccagata ctctccaaac ctggtcaact cctttgtgct
    17221 gaaatgtttc agcatctgta tcatcttgct cttgtacatc ctcaggcgct ctttacaagc
    17281 cccgaaagat acgagttttg tcggaaatgc gatatgtaag gtgcataacc aaatcaacaa
    17341 acaaattcac aatttattga ttttcaatta ttttcaattt tttcaataat aattgaataa
    17401 taaaatggca aaaaaatgct tcggaaaacg tggaaattgg agaaagatgt ccttttttta
    17461 gcctatggaa agtagtttct ttacattgtc gaaaaagacc tatggcatga ttagattgga
    17521 aaaccctttg aattcagctt tgcgagcaat gatggcttta tccctggtaa tagggctgtc
    17581 tgctgcagtg gatgctcaaa ccactacgat agctgttaat gacagcatta agatttgtga
    17641 gggaaccgac gtgatgatcg atgtgctgtt gaatgatatt gatccggata ttgagatcct
    17701 tgaaacagat attattgcgg gacctacctc gccgttgatc gactatgatg atgatgcact
    17761 tcctgaaggc agttataata tttacattga tcccactttt gttggtgaag attatattct
    17821 gtatgaggta tgcggtgaag atgacctttg cagcatgggt gtaatagtta tcatagtagc
    17881 aggaacagat gcatgtgttt ggcccggaga tgcaaatcag gataatattt gtaatgctgc
    17941 agatatatta ccaatcggtt tgtattacgg gttgatggga cctgaccgcg atgatgatga
    18001 tggttcatgg gaaaatacat ttgcagatga gtggacagat gtgccgggag cttattatcc
    18061 caatccaaaa tattcagata tcaatggtga tggtattgtt gatggcaatg acactactta
    18121 cctcgttgcc aattacggtt tcatcagcga tgtatatacg ccgtatgaag ctgtcggcgg
    18181 tgctgatgac ccggcaatat cacttggtat tttcagcgat acaatagaaa caggaacagc
    18241 agttaccatt ccgatatatt tcggaactga tttaattcct gcttccggca tttatggtat
    18301 cgcattcgaa ttatcttatg acgctacatt aatagatcca tcaagcgtgc atgtatcatt
    18361 ctatgacagt tggctgggaa caatgggtga tgacatgttt gcattgcaac ataacgatga
    18421 ggctacagga gttgtagaag tagccgtaac acgcaataat caaattgcca gaaccggggc
    18481 cggatatttt ggtcaggtaa gttttgtgat ggaagataat attgccggaa aaacaacagg
    18541 tcaaataaca tcaacagcaa gcttctgtat cagcaaacca aacgccgttg acggactcgg
    18601 aaccattaca ccggtgcaga tcagttgcga ctcggttgta gttattgatt tcagcaacag
    18661 cattgataat acaagttcgc gtggtattca agtttttcca aacccggcaa gtgctgaatt
    18721 aacggttaca cttcctgaaa gcgaggcaac catacattta atcaatgcac ttggtcagga
    18781 aattatgact atgcaagcta acaacacaca gattacaatt cctgtagaat caattcctgc
    18841 aggtacttat atgattcaaa tttcaggagc acatattaat cacacacaac aatgtatcat
    18901 tcaacattaa aaattaatac catgagaagt tggataacac tattcacaag tttatttatt
    18961 ttttcagttg cgcaagcatc aaatccggct gcagattgtg ttgcagcctt tgaatatgaa
    19021 gtaagcggcg ccactgttta ttttattgat ggctcaacag cagatcccgg tccgatctta
    19081 gaatggtttt gggattttgg cgatggcacc acatctacgg aatcaaaccc cgtgcacact
    19141 tatgccgaac ccggtgaata tgatgtttgt ttaaccattc actccgatgg aggttgcttt
    19201 gatgataaat gcgaaagcaa tattcctgtt gatggcggtg gcgctgattg tgttgtgacc
    19261 gcatcgttgg tcgcaattga tggttcatca ggacatttta ttgcagaagt aacgcctgca
    19321 gccgatatag ttacctacac ctggaactgg ggtgatggcg gaccactata tagcgaaaca
    19381 acaacaggtg cggcatcaga cccttggcat acctattcag atccgggctt ctatacagtt
    19441 tgcgttacca ttactaccgg tgccggatgt acagatgaat attgttttga aattgaaata
    19501 cctgccgccg gcgattgcta tgctctttat gaatataccc tagatggtgg tttagtgcat
    19561 ttttatgaag ctgcaggtgg tggcgatatt gtttcttatt cctgggattt cggtgatggc
    19621 agcataagca cagatccaaa cccatctcat atttgggcag ctgaaggaac ttaccttgtt
    19681 tgccttacca ttgaaacagc aaccggatgc agcgacacct attgccatga aattactatt
    19741 ggtggtggtg gtgattgcac ttccgatttt gaattcgacc tcgatggatt aacagttcat
    19801 tattttgaaa ctgcgcacgc aggcggtggt ggtgcagata tcgtttctta ttattgggat
    19861 tttggtgatg gcacttcatc agatgcacca aatccaattc acacatacac cgaagaagga
    19921 acttatttag tttgccttac cattgtaaca aacaccggtt gcacaagtac atattgtcat
    19981 gaagtacatg ttgaactacc taccggtgat tgtcttgcat cctacgttgt ttcatcaatt
    20041 gaacttacac ctgatggtta tgtaatacat atcgaaaata cttctacatc aggaggtgat
    20101 attacaacta caacctggta ttatggtgat ggcactattg gcgatagcta cgatgcagag
    20161 catgtgtata ccgaaagtgg tacgtatatt atttgcctgg tgatagcaac tgcagatgga
    20221 tgtgttgatg agtactgcac atctgtagtt gttggcgaac cgggatgcag cagcttcttt
    20281 gaatttacaa ctgacggacc tgccgtacat tactttgaag gtgccgatgg tggtggatat
    20341 gatattgtta gttatttatg gtcgtttggc gatggcactt ttagcgatgg accaaatccg
    20401 attcacattt ataccgaagc aggtacttat gaagtttgtt taaccatcac tacagcaagt
    20461 ggttgcgtaa gtacttattg ccatatcgta attgtagaag aaggtggtga attctgcgcg
    20521 gcaggattcg aaactgatgg cattgaagaa acaccggatg gatggatggc ccatttcgcc
    20581 aatacttcca ttggttcaga cctataccat tggacgtttg gcgatggcgg tgtttctgaa
    20641 ttgccaaatc ctagtcactt atttgaaact gcaggcatct acgctgtatg cttaacaatt
    20701 ggtgttggtg gtggcgattg cttcgataat tattgtgaag aattgtttgt tggcggcgat
    20761 gatgattgcg tgaatgcagg catcatagat tctactatgg attgcgttga agtttacgaa
    20821 ccggtttgcg gttgcga
//
LOCUS       DAOQHY010000042        20640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1160_length_20640_cov_5.020743, whole genome shotgun sequence.
ACCESSION   DAOQHY010000042 DAOQHY010000000
VERSION     DAOQHY010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 20640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20640
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1160_length_20640_cov_5.020743"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3489)
                     /locus_tag="PLL28_05220"
     CDS             complement(<1..3489)
                     /locus_tag="PLL28_05220"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNF68755.1"
                     /translation="MKLSCANLVVGLFILLVSFLTPTLHLNATSPSLPDGNDLRQLTS
                     ASFTDPANNFMVNIEGNAFNITHKENGWSLDARLTGMSRANATYPIEVMAGNVNNSKI
                     LTFHDAKIDIIYQLTSMGMRQDFVLKEKPAGEGDISLQLAFSSAQIEFSCNGSTLTGS
                     LNEIPVYAYTALKVWDANNAPLAASMQWHEGVLSLILNDDNAVYPITIDPVSITPNAI
                     LTGNMLSSQYGNDIDNAGDVNGDGYDDLIVGSIFYSNGQSSEGAAYIYLGSASGISTT
                     PQIILESNQNSANFGKAVAGAGDINNDGYDDIVIGAYNYDNGQTNEGRAYIYMGSASG
                     ISATPALTLEVNQAQCNFGSSVAAAGDVNNDGFADVIIGAYNYDNGQTNEGRAYVYHG
                     SATGLNATPVSIMESNSAQAYFGWSVGGDMDVNGDGFDDVIVGAYNQSNGQANEGLAY
                     VFHGSASGIITTPAATREMNQVNAFFGESVSNAGDVNNDGYDDVIIGAYLFDNGMSTE
                     GAAFIYHGSPTGISAAPITQLEANIMFAYFGYSVDAAGDINGDGFDDVIVGAYIFEYD
                     QQDEGRAFVFPGSVTGVSDVPDAVMERNEINANFGYAVCGGGDFNNDGFSDVAVSAPK
                     KDFAVTDAGGVFVYHGSNCTPHLLFEDFDGDGYGAFNYPIVSCDATAAGYAVNNDDCV
                     DDNAAQNPTTVWHLDADGDGYYIDDPAGITQCESPGTGYAFAGILGGDDCDDANPAAN
                     PGATEMVNGVDDNCDGIIEPWAWEGAVTLEGNQTSAYFGSAVSSAGDVNNDGYDDIIV
                     AANGWTDVQSDEGAIFIYHGSATGVSPTPNTSIQSNYAFAYFGYSVAAAGDVNGDGYD
                     DIIVGATNYELGQSNEGAAFIYHGSATGINTTPATILQADQADAFFGYDVAGNADLNN
                     DGYDDVIVSAYMFDNGQTNEGRVFIYYGSPSGVNSTPVLTLESDQPNAYFGRSIAING
                     DVNNDGYEDLVIGASHYDNILSGEGRVFVYLGTAAGIDPLQVSYHESNQTLAYLGRSV
                     AMSDVNGDGYDDVIAGAHYYDDTEINEGAIFVFNGSASGVSTTADDIIETNQGDAFLG
                     ISLASLDDINNDGYDDIITGATGFDGAEEDQGKAFQFNGSASGLSPSPEAGVFERAQY
                     KADYGVAVANAGDVNGD"
     gene            3571..4824
                     /locus_tag="PLL28_05225"
     CDS             3571..4824
                     /locus_tag="PLL28_05225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68756.1"
                     /translation="MKLKEYGLAAAAILASGDVSNQTAMYQDIDPDINIPAGDPFGWF
                     YSIANIDLNHDGVDDFRFRLEGFTTYTGWDAVGSFIQAAAIPLGSNQVQIASSQYIGW
                     CSIGWNAANFANVANYSNGQMIHANPTAGSFIEPINEEQSHQNLFIQRVKGYFASSWS
                     YFGDCDNPEDFYMFTGGFWFHADSRVIPFKVKDGGNLYTGWIRLSVDEGSLNIEDYAI
                     SMSPEANIVAGSLTGTVTAQAPNPGLVTVATTKANLTWSSVPGAIKYRFRYRPVGATV
                     WITKMTTTTSKLIKSLTCGTAYEWQVQAVYDNTPALNSEWSDVVNFSTSSCRIGDENT
                     YVEDVIVYPIPASNSITIETVSPAETIDAFVELYNLQGSLIKSYTTNSKEFTMDVSDV
                     APGTYMVVINNSNEEPIVQKVIIAR"
     gene            complement(4904..7060)
                     /locus_tag="PLL28_05230"
     CDS             complement(4904..7060)
                     /locus_tag="PLL28_05230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68757.1"
                     /translation="MIKISTLQILGICMLIMRISTMTAQVTYQWQQAHGGSVNDGAYS
                     INATSDGGYIVGGYSYSGISGDKTMASFGGEDYWVLKLDVDGNIQWQKAYGGSGLDRL
                     NKVVQITDGGYLVGGYSQSMISGNKTENSYAGTQDVWVLRLDSLGNILWQTCLGGSGI
                     DMLEDVNETADGGCIIGAYSTSGNSGDKTEGSKGVYDYWIIKLNSTGGIMWQNTIGGT
                     GSDYCRGAQQLPDGGYFISGYSGSPISFDKTEACVGPFNNNDAWIMKLDASGNIIWQN
                     VIGGSSNDNLTAAKLTPDNGAILALYSSSNISGDKSENTIAGVTTDADYWIVKVDAAG
                     NVMWENTIGGIDVDLCYDVTLMSDGGYALAGYSLSPMSGDKTTGVYASSFDYWIVGVD
                     ALGNVLWDRNYGTYLDDYCYGITTGADGMLVAAGDAESVTTGDKIVTGNGWKDFWLIK
                     LRNVCIPTDEVCNDIDDDCDGIIDEGVVENVYIVDPGITTVCQGTPVNMASVFTAGVP
                     QWYRNGVAIPGAIGIYYTATVSGNYTCVTSSDCGTDTSNVVHITVNKNPKASISAGGP
                     TTFCMGGSVTLTEMPSAGCTYQWMKGASLVAGATSNVYVATTTGNYKCLVTKTATGCF
                     KTSNTIPVNVICKLDGSDNISMSVFPNPTHDLIMLEIPENEIVDVWIYDQSGKLLTSL
                     NAFESGDISVENLPAGTYLVKATNDLHQWNAYFIKQ"
     gene            complement(7169..9367)
                     /locus_tag="PLL28_05235"
     CDS             complement(7169..9367)
                     /locus_tag="PLL28_05235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68758.1"
                     /translation="MITFKHMRPSLFFILSVAITQISFGQAPAIEWQNTIGGSSYEDL
                     RSLEQTSDGGYIIGGVSTSPISGDKTEAAWPGGPNGFDFWILKLHADGTIAWQNTIGG
                     TNDDYLYVVKETPDGGFICGGYSASGISGDKTEGSLGGKDYWVLKLDALGNIVWQNTI
                     GGSLDDILYDIALTTDGGYLLGGYSYSSISGDKTENNMGIASTDDYWVVKLNGDGTIA
                     WQNTIGGTSQDYLYKVLALADGGFLLGGTSYSGLNGDKTEVSMGGADYWIIKINSTGS
                     IVWQNGIGGSSNDNFRSFGLTTDGGFIVAGDSRSLVSGDKTETNFDNVTFYSDFWILK
                     LNSTGSIVWQNDIGSDRDDYPMDIMQTTDGGYFIAGNSVSALLGDKTEGVIGGGTIFY
                     DFWVMKLNATGSILWQNVIGGTNYDAPYDADITDDGGFIVGGHSYSGISGDKTEANMG
                     ASYGDYWVFKLESTCTPLPEVCNGMDDDCDGLIDDGITLSISIAAGGPTSFCQGSNVV
                     LTATHTGTSLQWKKNGANIAGATGTTYTALQTGLYTCTTTSLCGTTTSAGINVTMNKN
                     PKATITAGGTATFCAGGSVTLTEVPSAGCTYQWYKGAAPIAGATSTTYVATTAGSYKC
                     RVTKTATGCLKNSNAISVSVPCKEGEEIIAEELQIAVYPNPANDAVTLVLDQNLTTEI
                     FVTNVIGETIMKLQGDSELIEMDIHQLASGVYFVHVVQNGKMQTVSFCKN"
     gene            complement(9374..11557)
                     /locus_tag="PLL28_05240"
     CDS             complement(9374..11557)
                     /locus_tag="PLL28_05240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68759.1"
                     /translation="MKKLKFQHFLALAFLIGIQHSLIHAQIININWQQTAGGTATDGV
                     YKMAPTSDGGVITGGYTYSGVSGNKTDGTYGMGDYWVVKYDASGNLQWQKTYGGTGYD
                     KLEDIVQTSDGGYLLGGYSNSPVSGNKTEGLISGSIDIWVIKIDAAGTIVWQNDIGGN
                     QSDYLGSISETADGGCIVGAYSSSPISGDKTEGSKGLTDYWIIKLNSTGAISWQNTIG
                     GSASDYCFNAAQTADGGYLISGDSNSPVSFDKTESYTGVSANNDIWLLKLDAAGNIIW
                     QNVIGGTGTEACFEMCVTTDGGAILAAQSDSPISGDKTENTISGLVGDMDFWAVKVSS
                     LGAVQWDNTLGGTAADYCHGLTLMPDGGYALAGYTLSPAGGDITVGSFLGTSDYWVVR
                     LAADGTKLYDRTLGGSGTEYCYDLTTANDGKLIVGGTSSSSISGDKTSANYGVYDFWI
                     VKLSDACYPSIEICNAIDDDCDGLTDDGVVETISIAAGGATIVCQGTPVVLTATHTGT
                     SLQWKKNGVNIGGATGITYSATQTGNYSCTTTSACGTATSSSISVTVNKNPKATISAG
                     GPTSFCAGGSVTLTETPSAGCSYQWYQGATPIAGATSTSYVVTTAGNYKCRVTKIATG
                     CFKNSNGILVTVPCREGEELLMQIDNGFALYPNPASENVHIDLIDNINPGTELIITDA
                     MGNIVLKQQVIESTFDVSISLLPSGMYVARIINGQQLHSKTMIIQ"
     gene            complement(11783..13924)
                     /locus_tag="PLL28_05245"
     CDS             complement(11783..13924)
                     /locus_tag="PLL28_05245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68760.1"
                     /translation="MLNLLTKHATAALLLAIPFNLFAQAPAIEWQNTIGGTGYDFLNT
                     ISQTSDGGFIMGGYSASGIGGDKSEDNWGGDDYWIVKLNADGTIAWENTIGGGNFDRL
                     YSVEETADGGYILGGQSLSGGGWGDKSESNMGGYDYWVVKVNALGVVQWDNTIGGTGN
                     DQLYNAQPTSDGGYILAGTSDSGISGDKTENRVGNSDYWLVKLNSSGSIVWQNDIGGV
                     MFENLYSAYETTDGGFILGGTSTSGISGDKTAANIGGVDYWIVKVNASGTLVWEKTIG
                     STGSDYVYTCIATADGGSLAVGMSDGIANGNKTEAANGLFDYWLVKLDNSGNITWQNS
                     IGGTGNDYTFVDAGFQQNDGNYLIAGYSQSTISGDKTEANTGSWDYWILQLNSTGNIL
                     WQSVVGGSSGDYANSIGTTFDGGVIVGGYSYSNAGGEKTENSIGDADYWVVRYEGNCV
                     PTSEICNGLDDDCDGAIDESIAMFATAYATGPTTFCQGGSVVLNSSYSGDDVQWMKNG
                     VNIPGATSASYTATQKGSYSIITTTDCGAAVSAVVYVNVNKNPSASISASGPTSFCAG
                     GSVTLTEVPTAGCTYQWFKGAVPIAGATSTSYVATMAGNYKCRVTKTATGCLKNSNAI
                     AVTIVCKESVEIGLTLTVSPNPASDYIDIISTNSKHADITIVDAIGRVVLTEMLEEGF
                     ISVDVSKLPAGIYYVRCLAGSEISTVRFVKQ"
     gene            complement(13944..16034)
                     /locus_tag="PLL28_05250"
     CDS             complement(13944..16034)
                     /locus_tag="PLL28_05250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68761.1"
                     /translation="MANMHLKNTIQALLVAFPLAVFGQAPVIEWENTIGGNAWDGYPN
                     AKQTPDGGYIIGGNSMSNISVDKAENNFAGSNDFWVLKLNSSGGIEWQNTIGGNGNDF
                     PGQTIPTTDGGYMVGGHTLSGISGDKTSPGYGLYDLWLVKLNSTGGIMWQKTLGGTND
                     EWWSDLHQTSDGGYIVGGQSLSGLGGNKSEVSQGGFDYWVVKLNAAGGIQWQNTVGGS
                     ADDLLSSIIQTPDGGYLLGGTSSSGVSGDKTEGTMGVGYSDYWVVKLNAVGNIEWQNT
                     IGGNLNDVLQSVDKTLDGGYILSGYSNSGISGDKTETSFSVHSMWVIKLDAGGNIVWQ
                     QTMNDAYYSEIHPTLDGGYILGAMFPYQIIKLDASGEVVWSSFYGSSCNLNDIEQTSD
                     GGFIVSGHSYDGILVVKSEPNYGPPDFWIMKLAPDFCVPVDEVCNSTDDDCNGLIDDD
                     VIENITITAGGPVTFCNGGFVELTATYSGTSLQWRKDGLDIPGATNANYTVTSKGTYT
                     CQTTSVCGSAVSTGIYVQVYKNPNAFISADGPTLFCPGGSVTLTETPSGGCTYQWYKG
                     ASAIAGATSTTYIATTTGNYKCRVTKTATGCYKNSNAIAVSVTCREGELLDADNVQIN
                     VYPNPAQSTITIQSSSEIPSTIQIRDMVGQLIIPEMSLQGQLELDVTAWPAGIYFLQS
                     TTENDIIVTQFIKQ"
     gene            complement(16055..18217)
                     /locus_tag="PLL28_05255"
     CDS             complement(16055..18217)
                     /locus_tag="PLL28_05255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68762.1"
                     /translation="MFNILIKCSSVILLLAFPVMLNAQAPIINWEHLFCDGAYAAPEI
                     VLMDDGGAVIGTSTYSIISCEKTEAGHGGLDYWIFRVDANGNILWQVVYGGSGDDKLT
                     SIKRTSDGGFIIAGTSNSPISGNKSEPRQDLTAPFSDDLWLLKLDSTGNIIWQNTIGG
                     NSTESEPYAEQSGSGYIVSCSSYSPISGDKMEGVIGGMSYNDYWIIKLNASGNIVWQN
                     TIGGDLADRVSTVHKTLDGGYICVGVSSSNISGDKTQNTIGPWGYSDYWVVKLNSSGA
                     LDWQKTIGSVNYDEGVDVIVEADGNYTLGGTSYNDFGANYQIVRINNTGATLWTNTIN
                     AAESYLESIASTQDGGYVLGGRSMAMVGGDKTSPSKPMNSYGWEGWDMWFVKTNNLGD
                     VEWDLSLGSGDDDGSGKIYESQDGGFYVIGNAGNDEEGDITDKAILADGAWLVKLTTD
                     TCVAAVEICNTIDDDCDGMIDDDITESITVAAAGPLTFCQGESVTLSATSYTGPILQW
                     KKNGEDIPGANSASYVATQSGIYTCQTSSDCAFAISEWLTVTVNKNPKATISASGATT
                     FCAGGSVTLTETPSGGCTYQWYKGATPIAGATSTTYVATTTGNYKCRVTKTATGCYKN
                     SNAIAVSVTCREGELLDADNVQINVYPNPAQSTITIQSSSELPSTIQIRDMVGQLLIP
                     EMSLQGQLELDVAAWPAGVYFLQYATENKIVISQFVKQ"
     gene            18745..>20640
                     /locus_tag="PLL28_05260"
     CDS             18745..>20640
                     /locus_tag="PLL28_05260"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF68763.1"
                     /translation="MRTLAVFIVLIGFVCSAMGQTATLSGTITDADTKEPLFGVNIIL
                     TGTNLGASADFDGTYTITGIPPGEYNVQFSFIGYEKTLFTQQKISAGEKKKLDVALKS
                     TILTLDDAVVVVGAKPLVDVEEAKTGSTLTSKEIDLQPVRQIQSVVNMQTGVVQSADG
                     VHIRGGRSYETGFYIDDVSAKDPLSGTGFGIDIGTNSISKVDVTTGGAGVEYGNATSG
                     IVNTQTKSGDETFEFNAGYKRDNLGFNKNDAACWNQQVMEMGMGGPLAFKKKLTFYST
                     LKLNFSDTFIPNSPYGEGAAADHVTSSLYPDSMWSPYQDNRWAGMLKLDYKFDPKKRL
                     SFTYLKSITINQDYNMLRVTGNDVTFNPGYQFLFALQPDNANTYTHDTNLESLKWTHT
                     TSNTFSYSILASRLFVKLRADANGNQWRPDVVNTEFDPESITTYPATVFNPDDSIVFV
                     NPGPGLYNNGGIATLWHDHYVVEYTLRASGNLYSKDTRNKLFFGTELKPQEYQWIDIT
                     RPWIGAPIQLADGSYSQSYRLGDISDVWKVNPVSGAFFVSDKLKYLGLIAEAGMRLEY
                     WFPGKFLDDAVANPNTTIADAFRQAYYDDSYAMGDRRFKMRLLPKFSASFPIRENQVM
                     FFNYGHST"
BASE COUNT         5913 a         4939 c         3820 g         5968 t
ORIGIN      
        1 atctccattc acatcgcctg catttgcaac agcgacacca tagtcggctt tatattgtgc
       61 gcgctcaaat acacctgctt ccggtgaagg tgataatccg gatgcgctgc cattaaattg
      121 aaatgctttt ccctgatctt cttctgcacc atcaaaccct gtagcaccgg tgataatatc
      181 gtcataacca tcattattga tatcatcgag cgatgccaga ctgataccta aaaatgcgtc
      241 accctggttt gtttcgataa tgtcatcagc cgtggttgaa acacctgatg cagatccatt
      301 gaaaacaaaa atagcgcctt cattaatttc agtatcgtca taataatgtg ctccggcgat
      361 cacatcatca tagccgtcgc catttacatc actcatagca acagacctac cgaggtatgc
      421 caaagtttga ttgctttcat gataagaaac ttgcagtgga tcaataccgg ctgcagttcc
      481 gagatacaca aacaccctac cctctccgga caaaatatta tcataatgtg aagccccaat
      541 aaccagatct tcgtatccat cattattaac atcaccattt atggcgatag atctaccgaa
      601 ataagcattt ggttgatcag attccaatgt cagtaccggt gttgaattca cacctgaagg
      661 agaaccgtaa taaatgaata cccgaccttc atttgtttgt ccattatcaa acatatatgc
      721 acttactatt acatcatcat aaccatcatt attcaagtcg gcattaccgg caacatcata
      781 accaaaaaat gcatcggcct gatcagcttg caaaatggtg gcgggagtag tattaattcc
      841 tgttgcagag ccatggtaaa taaaggcagc cccttcattt gattgaccta attcataatt
      901 agtggcccca acaataatat catcatatcc atcgccattt acatcacccg cagctgccac
      961 agagtaccca aaataggcaa aggcataatt actttgaatt gaagtatttg gtgttggcga
     1021 cacccctgtt gcagagccat gataaataaa tatggcgcct tcatccgatt ggacatcagt
     1081 ccaaccattt gcagccacta tgatatcatc atatccatca ttattcacat caccggctga
     1141 ggaaacagcc gaaccaaaat aagcagatgt ttgatttccc tctaaggtaa cagcaccctc
     1201 ccatgcccaa ggctctataa tgccgtcgca attatcatcc acaccattta ccatttccgt
     1261 tgctcccgga tttgcggcag ggttagcatc atcacagtca tcgccaccaa gaataccggc
     1321 aaaagcatat cccgttccgg gagattcgca ttgtgtgata cctgcaggat catcaatata
     1381 atatccatca ccatcagcat caagatgcca tactgttgta ggattttgtg ctgcattgtc
     1441 atcgacacaa tcgtcattat tcacagcata tccggcagcg gtggcatcac aagaaacaat
     1501 aggataatta aaggctccat aaccatcgcc gtcaaaatct tcaaataata aatgtggtgt
     1561 acaattactg ccgtgataaa caaaaacgcc tcctgcatca gtaacagcaa aatctttttt
     1621 aggcgcacta acagcaacat cactaaatcc gtcattatta aagtcgccgc caccgcatac
     1681 agcataacca aagtttgcat ttatttcatt tcgctccata accgcatcgg gaacatcgct
     1741 gactcctgtt acagaacccg gaaaaacaaa tgcacgacct tcatcctgtt gatcatattc
     1801 aaaaatatag gcaccaacaa taacatcatc aaacccatca ccattaatat cacctgcagc
     1861 atcaacggaa tatccaaagt atgcaaacat aatattcgct tctaattgtg ttatgggagc
     1921 tgcactgatg cctgtcggag atccatgata aataaatgcg gcaccttccg tagacatacc
     1981 attatcaaat aaataggcgc caataatcac atcgtcatat ccatcattat ttacatcacc
     2041 tgcattactc acagattcgc cgaaaaatgc gtttacctga ttcatttcac gtgtagcggc
     2101 aggtgttgta ataattcctg aggcagatcc gtgaaataca taagcaaggc cttcatttgc
     2161 ctgaccatta gattggttat atgcacctac tattacatcg tcaaatccat caccatttac
     2221 atccatatca ccgccaacag accaaccgaa ataggcttgt gcgctattgc tttccataat
     2281 agaaacgggt gttgcattta gtcctgtagc agagccgtgg tatacatacg cgcggccttc
     2341 atttgtttgc ccgttgtcat aattatatgc gccgataatg acgtcagcaa atccatcatt
     2401 atttacatcg ccggcagcag caacagagga accaaaatta cattgcgcct gatttacttc
     2461 taatgtaagc gcaggcgtgg cactgatacc acttgctgag cccatataga tataagcacg
     2521 tccttcattt gtttgtccgt tatcgtaatt gtatgctcct atcacaatat cgtcataacc
     2581 atcattattg atatcgcccg caccggcaac agccttgcca aaattggcac tgttttgatt
     2641 cgactcaaga ataatttgtg gtgttgttga tatacccgaa gcagaaccaa gataaatgta
     2701 tgcggctcct tccgaagact gaccattgga ataaaaaatt gagcctacaa ttaaatcatc
     2761 ataaccatca ccattcacat cgccggcatt atcgatatca tttccatatt gtgatgagag
     2821 catgtttccg gttaaaatag cattcggtgt aatgctcacc gggtcgatag taatgggata
     2881 tacggcatta tcatcattca atatcaggga cagaacacct tcgtgccatt gcattgaagc
     2941 agccagcgga gcattatttg cgtcccaaac tttgagggct gtatatgcat aaacaggaat
     3001 ttcatttaat gaaccggtta atgtagaccc gttgcatgaa aattcaattt gagcggaaga
     3061 aaacgccagt tgtaaagaaa tatctccctc gcctgccggt ttctccttca gcacaaaatc
     3121 ctgacgcatt cccatggaag tgagctgata aataatatca attttagcat catgaaatgt
     3181 caatatttta ctattattta catttcccgc cataacctca atagggtaag tagcattcgc
     3241 tctggacatt ccggttaacc gagcatcaag cgaccatcca ttctctttat gggtaatatt
     3301 aaacgcattc ccttcaatat tcaccatgaa attattggca ggatccgtaa aagaggcact
     3361 tgtcaattga cgcaagtcgt taccgtccgg caatgacgga gatgtagcat taagatgtaa
     3421 agttggcgta agaaatgaaa caagtaaaat gaatagacca accacaagat tagcgcagct
     3481 aagtttcata aggataatat tttattttct aaaaataacg aaatattctt attttcacat
     3541 agtaaaatta ttaaccccaa aattttcctt atgaaactaa aagagtatgg tcttgctgcc
     3601 gcagccattt tggcctccgg tgatgtctcc aatcaaacag ccatgtatca ggacattgat
     3661 ccggatatca atattcctgc tggcgaccct tttggttggt tttactcgat tgctaatatt
     3721 gatttaaatc atgatggagt ggatgatttt cgtttcagac tggagggttt taccacttat
     3781 acagggtggg atgctgtagg cagtttcata caagccgcgg ccataccact aggttccaat
     3841 caggtacaga tagcatcatc acagtatatt ggatggtgta gtattggttg gaatgctgca
     3901 aattttgcca atgttgccaa ttacagcaat ggccaaatga ttcatgccaa tcctactgcc
     3961 ggttcattca ttgaacctat taacgaggaa caaagtcacc aaaacctctt tattcagcgt
     4021 gttaaaggtt atttcgcttc aagctggagc tattttgggg attgcgataa tcctgaagat
     4081 ttttacatgt ttaccggagg attttggttt catgccgatt ccagggttat tcccttcaaa
     4141 gtaaaagatg gcggtaattt atataccgga tggatacgtt tgagtgttga cgagggctca
     4201 cttaatatcg aggattatgc aataagcatg tcgcccgaag caaatattgt tgccggtagc
     4261 ctgaccggaa ctgttactgc tcaagctccg aatccgggtt tagttactgt tgccacaaca
     4321 aaagccaatc tgacatggag cagtgtgccg ggtgctataa aatatcggtt cagatatcgt
     4381 ccggttggtg ctactgtttg gattaccaaa atgaccacca caacgagcaa actgattaag
     4441 tcactaactt gtggaacggc atatgaatgg caggttcagg cagtttatga caacacacct
     4501 gctttaaatt ctgaatggag tgatgttgtg aatttcagca catccagttg taggataggt
     4561 gatgaaaaca cttatgttga agatgttata gtgtatccga tacccgcatc aaattcaatc
     4621 acaattgaaa ctgtttctcc tgcagaaact atagatgcat ttgttgagtt atataaccta
     4681 caaggatctt tgataaaaag ctacacaacc aattcgaagg aattcactat ggatgtgagt
     4741 gatgtagctc cgggtactta tatggtagta attaacaata gtaacgagga acctattgtg
     4801 cagaaagtga tcatagccag gtaaaaaaaa tcgaataaaa taaaaaggcc ataactacta
     4861 ttcatgatgg cctttttttt gacctttgct cgggattaaa atgttattgt tttataaaat
     4921 aggcattcca ttgatgcaaa tcattcgtgg cctttacaag ataagttcct gcaggaagat
     4981 tttctacaga aatatcgcca ctttcgaatg cattcagtga ggttaacagt ttacctgatt
     5041 gatcatatat ccatacatca acaatttcat tttcaggaat ttcgagcatg attaaatcat
     5101 gcgttggatt tggaaatacc gacattgaaa tattatcact gccatccaat ttgcaaataa
     5161 catttaccgg aatagtattt gatgttttga agcatcctgt tgcagtcttt gtaacgaggc
     5221 atttataatt tccggtagtg gtggcgacat acacattgga tgtagcacct gcaaccaacg
     5281 atgctccctt catccattga tatgtacagc cggcggatgg catttcagtg agagtaacac
     5341 ttccacccat acaaaatgtg gttggtcctc cagccgaaat acttgcttta ggatttttat
     5401 tgaccgttat atgcaccaca tttgaggtat ctgtgccgca atcgctcgaa gtaacacaag
     5461 tgtaattacc ggaaactgta gctgtgtaat agataccaat tgcccccggt atagcaacac
     5521 catttctata ccattgcggc accccggcgg taaatacaga tgccatattc acaggtgtac
     5581 cttgacaaac agttgtgatc cccgggtcga cgatatatac attctcaaca accccttcat
     5641 caataatacc atcacaatcg tcgtcgatat cattgcatac ttcatctgtc ggaatacata
     5701 cattgcgcag tttaatcaac caaaagtctt tccatccgtt tcctgtcaca attttatcac
     5761 ctgtagtcac tgattctgca tcccctgctg ctaccaacat accatcggcg cctgtggtaa
     5821 taccgtaaca ataatcatcc agataggtgc cgtaatttct atcccagagc acattaccca
     5881 gtgcgtctac acctactatc caataatcaa aggatgatgc ataaacacct gtagttttat
     5941 ctcctgacat tggcgataag gagtatcctg ccaaagcata tccgccatct gacataagtg
     6001 taacatcgta acatagatca acatctatac caccgattgt attttcccac attacattcc
     6061 cggctgcatc aacttttact atccaataat cggcatctgt ggttacgcct gctatggtat
     6121 tttcagattt atcgcctgaa atattcgatg aagaataaag agcaagtatt gcaccattat
     6181 caggtgttaa tttagctgcc gtcagattat catttgagga accgcctatt acattctgcc
     6241 atatgatatt tcccgaagca tcgagtttca tgatccaggc atcattgtta ttaaatggtc
     6301 ccacacatgc ttcagtttta tcgaagctta ttggcgaacc tgaatatccg ctgataaaat
     6361 atccgccatc aggcaattgt tgtgcgcctc gacaataatc agatcctgta ccaccgatag
     6421 tattctgcca catgatgccc ccggtgctat tcagtttaat aatccaataa tcatatacgc
     6481 ctttcgaacc ttcggttttg tctccgctat ttccgcttgt agaataggcg ccaataatac
     6541 aaccgccgtc agcagtttca tttacgtcct caagcatatc aatcccactg ccaccgaggc
     6601 atgtttgcca cagaatgttt cctaaggaat ccaatctcag cacccataca tcttgtgtgc
     6661 cggcataaga attttctgtt ttattaccgg aaatcattga ttgggaatat ccgccaacga
     6721 gatatcctcc atcagtaatt tgcactacct tgttcaacct gtcaagccca cttccaccat
     6781 acgctttttg ccattgaata tttccatcga catccagttt aagtacccaa taatcttcac
     6841 cgccaaatga ggccattgtt ttgtcgccgg aaattccgga atagctataa ccaccaacga
     6901 tatatccgcc atctgatgtt gcgttgatgg aataggcccc gtcattaact gagccaccgt
     6961 gcgcttgttg ccattgatag gttacttgag cagtcattgt gctgattcgc attatcagca
     7021 tgcagatacc taagatctgc aaagtagaaa ttttaatcat ggggggtaaa ttttacgata
     7081 aagctagcga atccatattg gatttgtcaa atttcaagcc caaaatagcg caaaatcacg
     7141 gtttcccgtg aaggaaaaag agcgaatttt agttcttaca gaaagacacc gtttgcatct
     7201 tcccgttctg caccacatgc acaaaataca ctccggatgc caactgatga atatccattt
     7261 ctatcaactc gctgtcgcct tgcaacttca taatggtttc gccgataaca ttggtaacga
     7321 aaatttcagt tgtcagattt tgatccaaaa ccagggtgac cgcatcgttg gcggggtttg
     7381 gataaacagc tatttgcaat tcttcagcaa tgatctcctc cccttcctta catggcaccg
     7441 aaaccgaaat agcattcgaa ttttttaaac acccggttgc cgttttggta acgcgacatt
     7501 tgtaacttcc tgcagttgtt gccacgtagg tagttgatgt tgctcctgct atgggtgcgg
     7561 ctcctttata ccattggtaa gtacatcctg ctgaaggcac ctctgtcaat gtaacacttc
     7621 caccggcgca aaaagtagct gtaccgcctg ctgtaatggt tgccttcgga tttttattca
     7681 tggttacatt aattcctgcg gatgtagttg ttccacacaa acttgttgtt gtgcaggtat
     7741 ataaccccgt ttgcaaagca gtataagttg ttcctgtggc gcctgcaata tttgcaccat
     7801 tctttttcca ctgtagtgaa gttcctgtat gcgttgcagt taacacaaca ttactacctt
     7861 gacaaaaaga agtaggccca cctgctgcaa ttgaaataga aagtgtaatg ccatcatcaa
     7921 ttagaccatc gcagtcatcg tccataccat tgcaaacctc gggtaatggc gtgcaggtgc
     7981 tttccaattt aaacacccaa taatcaccat aagaagcacc catatttgct tcagttttat
     8041 cacctgaaat accggaatag gaatgccccc caactataaa accaccatca tcagtaatat
     8101 ctgcgtcata aggtgcgtca taatttgttc cgccaattac attttgccat aatatactac
     8161 ctgtagcatt caacttcatc acccagaaat cataaaagat ggttccaccg ccaataacac
     8221 cttcggtttt atcacccaat aatgcagaaa cagaattgcc tgcaataaaa tacccaccat
     8281 ccgtagtttg cataatatcc atcggataat catcgcgatc actgccgata tcgttttgcc
     8341 atacgatact accggtacta tttagtttca atatccaaaa gtctgaatag aaagtcacgt
     8401 tgtcgaaatt ggtctctgtt ttatcgccac taaccagcga cctagaatcc cccgctacaa
     8461 taaatccgcc atctgttgtc aatccaaagg acctgaaatt atcattactg ctgccaccga
     8521 taccgttttg ccaaacaata gaaccggtgc tgttgatctt tatgatccaa taatctgcac
     8581 cacccatgga tacttccgtt ttatcgccat tcaatccgga ataggatgtt ccacctaata
     8641 aaaatccgcc atcagcaagt gccaacacct tatacaaata atcttgagat gttccaccaa
     8701 tagtgttctg ccatgcaata gtgccatcac cattcagttt aaccacccag taatcatcag
     8761 tggaggcaat gcccatatta ttttccgttt tatcgcccga aatggatgaa taggaatatc
     8821 ctccaagcag ataaccacca tcagttgtta aagcgatatc atataaaata tcatctaaag
     8881 acccacctat agtattctgc caaacaatat tgccaagtgc atccaatttg agcacccaat
     8941 aatcttttcc gcctaatgaa ccttcagttt tatcgcccga tatgccactt gcagaatatc
     9001 ctccgcagat aaagccacca tcaggtgttt ctttaactac atacaaataa tcgtcgtttg
     9061 ttcctccgat ggtattttgc caggcgatgg taccatccgc atgtaatttc aatatccaga
     9121 aatcaaatcc atttggacca ccgggccatg cagcttccgt tttatcacct gaaatgggcg
     9181 aggtagatac acctccaata atatagccgc catcagatgt ttgctccagt gaacgcaggt
     9241 cttcatatga acttccaccg atggtatttt gccattcgat agcaggtgct tgaccaaaag
     9301 aaatttgcgt gatcgctaca gaaagaataa aaaataacga aggtcgcatg tgtttgaatg
     9361 ttatcatgga aatttattga atgatcattg tttttgaatg taattgttga ccattgataa
     9421 ttctcgctac atacatgcct gacggcaaca agcttatcga aacatcaaaa gtgctttcaa
     9481 ttacctgctg ttttaacaca atatttccca tcgcatcagt aattatcaat tcagttcccg
     9541 ggtttatatt gtcaattaaa tcaatatgca cattttctga tgccgggtta ggatatagtg
     9601 caaatccatt gtcaatttgc atcaatagct cctccccttc cctgcaaggc acagtcacca
     9661 aaatcccatt cgaattttta aagcatcctg ttgcaatttt ggtaacgcga catttataat
     9721 ttcctgcagt ggttactaca tatgatgtag aggttgcgcc tgcaattgga gtggcgccct
     9781 gataccattg atagctgcat cctgcagatg gtgtttctgt caaggttaca ctgcctccgg
     9841 cacaaaacga ggtaggtccg ccggcagaaa ttgttgcttt aggattttta ttcactgtaa
     9901 cagaaattga ggaagatgtt gcagttccgc aggcacttgt ggtagtacag gaataatttc
     9961 cagtttgggt agcactatat gtgatgcctg ttgcacctcc gatattcaca ccattctttt
    10021 tccattgcag tgaagtgccg gtatgtgttg ctgtcagcac aacgggtgta ccctgacaaa
    10081 caattgtcgc accgcctgct gcgatagaaa ttgtttctac cacgccatca tctgttagtc
    10141 cgtcacaatc atcatctatt gcattacata tttcgataga tggataacaa gcatctgata
    10201 attttacaat ccaaaaatca tatacgccat aattagcaga agttttatca ccactgatag
    10261 atgatgatga agtaccgcca acaattaatt ttccatcatt ggctgttgtc aagtcataac
    10321 aatattcagt tccactgcct cctaaagttc tatcatacag tttagtgccg tcagcagcta
    10381 gtcttaccac ccaataatcg ctggttccta aaaatgaacc aacggtaata tctccaccgg
    10441 caggcgaaag agtatatccc gccaatgcat aaccaccatc aggcataagt gtaagtccgt
    10501 gacaataatc tgccgcagtt cctccaagtg tattatccca ttgcactgcg ccaagtgagg
    10561 aaacttttac ggcccaaaaa tccatatcac caacaagtcc cgaaatggta ttttcagttt
    10621 tatctccgga tatgggtgaa tccgattgtg ccgctagaat agcaccgcca tctgtggtaa
    10681 cacacatttc aaagcaggct tcagtaccgg ttccaccaat aacattttgc caaataatat
    10741 ttccggctgc atctaatttc aacaaccata tatcattatt tgcagataca cctgtatatg
    10801 attcggtttt atcaaaacta accggtgaat tagaatcacc tgagatcaaa taacccccat
    10861 ccgcagtttg tgcagcattg aaacaataat ccgaagcgga tccgccgatg gtgttttgcc
    10921 agcttatggc acctgtacta tttaatttaa ttatccagta atctgttaac cctttcgaac
    10981 cttctgtttt atcgccggaa atgggactcg atgaatatgc tcctacaatg catcctccgt
    11041 cggctgtttc acttatggag ccgaggtaat cactttgatt tcctccaata tcattttgcc
    11101 atacaatggt gccggctgca tcaattttta ttacccaaat atcaattgac cctgaaatta
    11161 atccttctgt tttatttccg gataccggtg aatttgaata tcctcccagg aggtaaccgc
    11221 catcacttgt ttgcactata tcctcgagtt tatcatatcc tgtgccgcca tacgtctttt
    11281 gccattgcaa attaccggaa gcatcatatt ttacgaccca gtaatcgccc attccatatg
    11341 tgccatcagt tttatttcct gatactccgg aataggtgta tccgccggta atcacgccgc
    11401 catcagatgt tggagccatt ttataaaccc catctgtagc tgttccgccg gcagtttgtt
    11461 gccagttaat attgataatc tgtgcatgga ttaaggaatg ttgaattcct atcaaaaatg
    11521 ccagtgcaag gaagtgttga aatttgagct ttttcattgg tgtgtatatt tagagatcag
    11581 tcgaccattt tgttatttca aacacaaata tgtctaaaca tatattgaat tttatttaat
    11641 atatcatggt cctcctgaag ttcaaattta cgcatctcaa tagcaatacc atgtcgcttg
    11701 gcgttaaaaa tgacaagtgc agccaatgtt gtataagtgt tcggcaggaa tgagtaacag
    11761 cgaagggaaa gcgcctgtct gtttactgtt taacgaacct tacggtagag atctcggaac
    11821 cggccaggca gcgaacataa tatattccgg cgggcaattt gcttacatct acggatataa
    11881 atccttcctc aagcatttct gtgagcacta ccctgccaat tgcatcaaca attgtaatat
    11941 cagcatgctt ggagttcgta ctgatgatat cgatgtagtc gctagccgga ttcggactta
    12001 cagtcagcgt taaaccaatt tccacggatt ccttacaaac gatggtaact gctatagcat
    12061 ttgaattctt taaacaacct gttgctgttt tagtaacgcg acatttataa tttccggcca
    12121 ttgtggcaac ataactggtt gatgttgctc ctgctattgg cactgcacct ttgaaccact
    12181 gatatgtaca tcctgcggta ggcacttctg ttaaagtgac actgcctccg gcacaaaatg
    12241 atgttggtcc tgaagcagaa attgaagcac ttggattttt attaacattg acatacacaa
    12301 cagcgctcac agccgcaccg cagtctgtag ttgttattat agagtagcta cctttttgcg
    12361 ttgccgtata actagcagaa gttgcaccag gaatattaac tccatttttc atccattgta
    12421 catcatcgcc gctgtatgat gaattcaaca ccacgcttcc tccttgacaa aatgtagttg
    12481 gccctgtagc ataagctgtt gcaaacatgg caatactctc atcaatggca ccatcacaat
    12541 catcatcaag gccattacat atttctgagg ttggtacgca attaccttcg taacgcacca
    12601 cccaataatc tgcatcaccg atactgttct ctgttttttc tccgccggca ttgctgtagg
    12661 aatatccacc tacaattaca ccaccatcga atgttgttcc gattgaattg gcatagtcgc
    12721 ctgaagaacc tccaacaact gattgccata gaatattacc tgtagaatta agttgtagta
    12781 tccaataatc ccatgatcct gtgttcgctt cggttttatc gccactaatt gtgctttgcg
    12841 aatatcctgc tattaaataa ttgccatcat tttgctggaa tccggcatca acaaatgtgt
    12901 aatcatttcc tgtaccgcca attgaatttt gccaggtaat atttccgctg ttatccaatt
    12961 tcaccaacca ataatcgaaa agtccgttcg cggcttcagt tttatttcca ttcgcaatgc
    13021 cgtctgacat gccaacagcc aatgaaccac catctgcagt tgcaatgcaa gtatatacat
    13081 aatcactacc ggtactgccg atagtttttt cccataccaa ggtgcctgat gcatttactt
    13141 ttacaatcca ataatcgaca ccaccaatat tcgcagctgt tttatcgccg ctgataccac
    13201 ttgtagatgt tccacctaaa ataaaaccac catcagttgt ttcgtatgcg gaatataaat
    13261 tttcgaacat cacaccgccg atatcatttt gccaaacgat ggaaccactg ctatttaatt
    13321 tcaccaacca ataatcagaa ttacctacgc gattttctgt tttgtcgccg ctgataccgc
    13381 tatctgaagt gcctgcaaga atatagcctc catcacttgt tggttgtgca ttatataatt
    13441 gatcattgcc tgttccacca atggtattat cccattgtac gacacccaat gcattcactt
    13501 tcaccaccca ataatcatat ccacccatat tactttccga cttatcgccc catccgccgc
    13561 cgctgagtga ttgtccgccc aaaatatatc caccatctgc agtttcttcc acagaataca
    13621 ggcgatcaaa atttcctcca ccgatggtat tttcccatgc aattgtacca tctgcattta
    13681 atttaactat ccaataatca tcaccacccc aattatcttc cgatttatcg ccaccgatac
    13741 cactggctga ataaccgccc atgataaatc cgccatctga cgtttgggaa atggtattca
    13801 gaaaatcgta gccggtgccg ccaatagtgt tttgccattc gatggccggg gcttgtgcga
    13861 acaggttaaa gggaatagcg agcaaaagcg ccgctgtcgc gtgtttcgtg aggaggttga
    13921 gcatgttaaa agtgtgtttt agcttattgc ttaataaatt gtgttacaat aatatcattt
    13981 tctgttgtgg attgcaaaaa gtaaataccc gcaggccatg ccgttacgtc caattccagt
    14041 tgaccttgca ggctcatttc cgggatgatg agttggccta ccatatccct tatttgaata
    14101 gttgatggta tttcgcttga agattgaatc gttattgttg attgtgcggg attgggatat
    14161 acattgatct gcacattatc tgcatcaaga agttctcctt ctctacaagt tactgatact
    14221 gcgatagcat ttgaattttt gtaacaaccg gttgctgttt tcgtaacgcg acatttgtag
    14281 ttgcctgtag ttgtggcaat ataggttgtt gatgtagctc ctgcaatggc agatgcacct
    14341 ttataccatt ggtatgtgca gccaccggat ggcgtttctg tgagtgtgac acttccgcct
    14401 gggcaaaaca aagtaggacc atctgcagaa ataaaagcgt taggattttt atatacctgc
    14461 acatatattc cagtagaaac tgcggatcca cacacactag ttgtctgaca ggtataagtg
    14521 ccttttgaag ttactgtgta attggcattg gttgcaccag ggatatctag gccatctttt
    14581 ctccattgaa gtgatgtgcc tgaataggta gctgttaatt caacaaagcc accattacaa
    14641 aatgttacgg gtcctcctgc cgtaatagtt atgttttcta ttacatcatc gtcaattaat
    14701 ccattgcaat catcatcagt cgaattacat acttcatcta caggaacaca aaaatcaggc
    14761 gcgagcttca taatccaaaa atcaggcggt ccataattgg gctcagattt aactactagt
    14821 attccatcat atgaatgccc actaacaata aatccgccat cagatgtttg ttcaatatca
    14881 ttcaggttgc aacttgagcc ataaaaactg ctccagacaa cttcacctga ggcgtcgagt
    14941 ttaatgattt gatagggaaa catggctcct aaaatatatc ctccatcaag tgtcggatgt
    15001 atttcgctgt agtatgcatc attcattgtt tgctgccaga caatgttgcc tccggcatcc
    15061 agtttaatta cccacatcga atgtacacta aaactggttt cagttttatc acctgagata
    15121 ccggaatttg aatatccact cagtatatag cctccatcca aagttttatc aaccgactgc
    15181 aatacatcat tcaaattccc accaatggta ttttgccatt caatattgcc aaccgcattt
    15241 aatttaacaa cccaatagtc gctatatcca acccccatgg ttccttctgt tttatcgccg
    15301 gaaactccgg aagaagaagt gccacccaac aagtaaccac catcaggtgt ttgaataatc
    15361 gaactcaaaa gatcatcagc acttccgcca acagtattct gccactggat accaccggcc
    15421 gcatttagtt ttacaaccca atagtcaaaa ccgccttggc ttacttctga tttattccct
    15481 cctaaacctg aaagtgattg cccgccgaca atataaccgc catcagaagt ttggtgcaaa
    15541 tccgaccacc attcatcatt tgttccacca agcgtttttt gccacataat tccgcctgta
    15601 gaatttagtt tgactagcca tagatcatag agtccataac caggagatgt tttatcaccc
    15661 gaaattccgg aaagcgtgtg tcctccaacc atatatcctc catcagtggt tggaatagtt
    15721 tgccctggaa aatcattacc gttcccgcca attgtatttt gccattctat acctccagat
    15781 gaattcaact taagcaccca gaaatcattt gatccggcaa agttattttc tgctttgtca
    15841 acagagatgt tggacattga atttccacca attatataac ctccatccgg tgtttgtttt
    15901 gcattcggat aaccgtccca tgcatttcct ccaatagtat tttcccattc gatgactgga
    15961 gcttgtccaa atacagcaag agggaatgca accaatagcg cctgtattgt attttttaaa
    16021 tgcatgttgg ccatgtgtgg taagtttatt taatttattg cttgacaaat tgtgaaataa
    16081 ctatcttatt ttctgttgca tattgcaaaa agtaaactcc cgccggccat gccgctacat
    16141 ctaattccag ttgtccttgc aggctcattt cagggataag gagttggcct accatatccc
    16201 ttatttggat agttgatggt aattcacttg aggattggat cgttattgtt gattgtgcgg
    16261 gattgggata tacattgatc tgcacattat ctgcatcaag aagttctcct tctctacaag
    16321 ttactgatac tgcgatagca tttgaatttt tgtaacaacc ggttgctgtt ttcgtaacgc
    16381 gacatttgta gttgcctgtt gttgtagcaa cataggtagt tgatgtagct cctgcaatcg
    16441 gtgttgcacc tttataccat tggtatgtgc aaccacctga tggcgtttct gttaatgtta
    16501 cactaccgcc tgcgcaaaat gtcgttgcac cggaagccga aatggttgcc tttggatttt
    16561 tattcaccgt cactgtcaac cactctgaaa tagcaaatgc gcagtcactg gatgtttgac
    16621 aagtataaat gcccgactgc gtagcaacgt atgaagctga atttgcaccc ggaatatctt
    16681 ccccgttctt tttccattgc aatataggtc ccgtgtaact ggtagctgaa agtgttactg
    16741 attcaccttg acaaaaagtg agtggtccag cggcagctac agtgatagat tcagttatat
    16801 cgtcatcgat catgccatca caatcatcat ctatcgtatt acaaatttca accgccgcaa
    16861 cacaagtatc agttgtcagt ttaaccaacc atgcgccgtc ggctaaaatc gctttatcag
    16921 taatatctcc ttcctcatca tttccggcat tccctattac ataaaaacca ccatcctgag
    16981 attcgtatat tttacccgaa ccatcgtcat caccgctacc tagtgaaagg tcccattcca
    17041 catcacccaa attatttgtt ttaacaaacc acatatccca tccttcccat ccgtagctat
    17101 tcataggttt tgaaggggaa gttttgtccc caccaaccat ggccattgac cgaccgccta
    17161 aaacataacc accatcttgt gttgatgcaa tactttcaag gtaagattca gctgcattaa
    17221 ttgtgttagt ccaaagtgtt gctcctgtat tatttattct aacaatctga tagtttgctc
    17281 caaaatcatt atagcttgtt ccgccaagtg tataatttcc atccgcttca acaataacat
    17341 caacaccttc atcataatta actgacccaa tagttttctg ccaatccaag gcaccactgc
    17401 tattcaattt aaccacccag taatcactat agccccaagg tccaatcgtg ttttgcgttt
    17461 tatctcccga aatatttgaa cttgataccc ctacgcaaat gtacccacca tctaatgttt
    17521 tatgcacagt tgacacgcga tcagcaagat cgcctccgat ggtgttttgc caaacaatgt
    17581 tgccggaagc gtttaattta attatccaat aatcattata agacatgcca cctatcacac
    17641 cctccatttt atcgcccgaa ataggagaat aggaagaaca agaaacaata tatccgctac
    17701 cgctctgttc agcatagggt tcggactctg tcgaattacc cccaatagta ttttgccaaa
    17761 tgatatttcc tgttgaatcc aattttagca accacaaatc atcagaaaac ggcgccgtaa
    17821 ggtcctgtct tggttctgac ttgtttcctg aaataggaga attagatgtg cctgctataa
    17881 tgaaaccacc gtcagaggtt cttttgatcg aggttaattt atcatcacct gaaccaccgt
    17941 aaacaacttg ccatagaata ttcccattgg catccaccct aaatatccaa taatccaaac
    18001 caccgtgacc ggcttcggtt ttttcacaag aaataattga atatgtggaa gtcccgatta
    18061 cagctcctcc gtcatccatc aacactattt cgggagcagc ataggcacca tcgcaaaaga
    18121 ggtgttccca atttatgatt ggggcttgtg cattaagcat tactggaaat gccagcagaa
    18181 gaattacaga agagcacttt ataaggatgt tgaacataaa atgtttttaa agcccaatca
    18241 tttgaataac ctaccaatat tacaaatttc agggcatttt aacaaatacc caagtcgagt
    18301 ggagatgggc aaatgcatgt acaaaataca ggagacagtg aagttgcatg gttttgaacc
    18361 tcacccccga cccctctcct ttccattcaa cctgcggttg aattaagggg aggggaggaa
    18421 tccattaata tcttattatt acctcaaaaa aattatatct ccccccccct ccccttagat
    18481 ttcgccatag gcgatatcgg aaaggagatg ggtcgggggt gaggttcaca cgaaatgcaa
    18541 tcccgttctc gattataagg cttataaaaa taatttaccg gacaacactg attagcagat
    18601 tagcaaatta gcaaatcagc acatcagcaa atcagcaaat tcccacatct cgcatatctc
    18661 gcacatctcc cacatcaatc cgccaattat tcaacactcc cccatttgca cacttcccca
    18721 cttccacact acctttatcc cctcatgcgc acactggcgg tttttatagt gctgatcggt
    18781 tttgtctgct ctgctatggg ccagaccgct accctatccg gcaccatcac cgatgctgat
    18841 accaaagaac ccttgtttgg ggtgaatatc atcctcaccg gaactaacct gggtgcttcg
    18901 gctgattttg atggcactta taccatcacc ggcatccctc ccggcgaata caatgtgcag
    18961 ttttcattca tcggctacga aaagaccctc tttacccaac aaaagatctc cgccggagag
    19021 aagaagaagc ttgatgttgc ccttaaatcg accatattga cgctagacga cgccgtggtg
    19081 gtggttgggg ctaaaccact ggtggatgtg gaagaggcta aaacgggctc tacactcact
    19141 tcaaaggaga tagatctgca gccggtgcgg caaattcaaa gcgtggtgaa catgcaaacc
    19201 ggtgttgttc aaagcgctga tggcgtgcat atcagaggtg gaaggtcata cgaaacagga
    19261 ttttatatcg acgatgtctc tgccaaagac ccactttccg gtacaggctt cggtatcgac
    19321 atcggcacaa atagtatttc taaagtagat gtcaccaccg gaggtgcagg tgtcgaatat
    19381 ggcaatgcca cctcaggaat cgtaaacaca cagactaaat cgggcgatga aacattcgaa
    19441 ttcaatgccg gttacaaacg cgataacctc gggttcaaca aaaacgacgc tgcctgctgg
    19501 aatcaacagg taatggagat gggtatgggc ggaccgctgg ccttcaaaaa gaagctcacc
    19561 ttctactcta ccctgaaact caatttctct gataccttca tccccaacag tccgtatggt
    19621 gaaggtgctg ctgctgatca tgtcacctct tcactgtatc ctgacagcat gtggagtccc
    19681 taccaggaca accgctgggc ggggatgcta aagctggatt ataagttcga ccctaagaaa
    19741 cgactgagct ttacctacct gaaaagtatc accatcaacc aggattacaa tatgcttcgg
    19801 gttaccggca acgacgtgac cttcaatccc ggctatcagt tcctgtttgc gctgcaaccc
    19861 gataatgcca atacgtacac ccacgatacc aacctggagt cgctgaaatg gacgcatacc
    19921 accagtaaca ccttcagtta cagcatttta gcttcgcgac tattcgtaaa actgcgtgcc
    19981 gatgccaatg gcaaccaatg gcggcccgat gtggtgaata cggagttcga tccggagagc
    20041 attaccactt atcccgccac cgttttcaac cctgacgact ctatcgtatt cgtgaatccg
    20101 ggaccgggat tgtacaacaa tggcggtatt gccactttat ggcacgatca ttacgtagta
    20161 gaatacacct tgcgtgcttc aggaaatctg tattcgaaag atacccgcaa taagctattc
    20221 ttcggcaccg aactaaagcc acaggaatat cagtggatag atatcaccag gccttggata
    20281 ggtgctccga ttcaactggc tgacggctcg tattcgcaga gctaccgact gggcgatatc
    20341 agcgatgttt ggaaggtaaa ccccgtttcc ggcgcgtttt ttgtatcgga taagctgaaa
    20401 tacctcggat tgatcgccga agccggcatg cgactggaat actggttccc gggcaaattc
    20461 ctagacgacg ccgttgcaaa tccaaatacc accatcgccg atgccttccg ccaggcgtat
    20521 tacgacgaca gctatgccat gggcgacaga cgcttcaaga tgcgcctgct gcctaagttc
    20581 tcggctagtt tccccatcag agagaatcag gtgatgttct tcaactacgg tcacagcacc
//
LOCUS       DAOQHY010000043        20412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1178_length_20412_cov_6.025102, whole genome shotgun sequence.
ACCESSION   DAOQHY010000043 DAOQHY010000000
VERSION     DAOQHY010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 20412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20412
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1178_length_20412_cov_6.025102"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..734)
                     /locus_tag="PLL28_05265"
     CDS             complement(51..734)
                     /locus_tag="PLL28_05265"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNF68764.1"
                     /translation="MSQIQFTRFNYLPPVIKNLLIINGLVYLATFTFPELKETLALHY
                     PSTPEFRPYQIVTHMFTHENFAHVFFNMFSLWMFGAVLENVWGPKRFLQYFLITGLGA
                     ALCFTAYHAYEVYQSVGTIFPSSSITDTPLRWNVNGLNYSAVGASGAIYGILVGFGLL
                     FPNTLIYIYFLLPIKAKYLVIILIAYELYTGFINDPNDNVAHFAHLGGALIGFIMIKY
                     WQKNSRRFY"
     gene            complement(743..2545)
                     /gene="mutL"
                     /locus_tag="PLL28_05270"
     CDS             complement(743..2545)
                     /gene="mutL"
                     /locus_tag="PLL28_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNF68765.1"
                     /translation="MGDIIHLLPDSIANQIAAGEVIQRPASAIKELMENAVDAGATEI
                     KVVVKEAGKMLIQVIDNGKGMSFTDARMCFERHATSKIRSADDIFSIRTKGFRGEALA
                     SIAAIAQVELKTRQQEHELGARLVIEGFEVKAHEQAQCPVGTNIQVKNLFYNVPARRN
                     FLKSNTVELKHIIDEFERVVLAHPDIFFSLQHNGVELFHLKAGNLRQRIIHLFGSNYN
                     EKLVPISEDTSVTNIRGFIGKPDAAKKTKGEQFFFVNNRFIRSPYLHHAVMSAYQELL
                     PEGSYPLYTIYIDIDPARIDVNVHPTKQEIKFEDERIIYTFIQAAVRHALAQYSVTPT
                     LDFEQDQTFANLPSFYKKPEEQPVSFKTVESRMNDIPQHQSSFKHDVASTNRMHTAFK
                     REVSGALHDDEVQSDMPTDGATPFQIHYQYVVTQIKSGFIVIDQQAAHERILYEKYVT
                     QLREKKHHSQQQLFPETLKLSGADFALMEMLLPDLQNLGFDIMPFGKDAFVVHGIPVE
                     FENGDINGVVEALLEQFKEDKSTLKLDRHEAIARSLARTNSIKRGQKLDERGMRHLAD
                     QLFACETPYTSPFGNLTFVTFTLEALARQFQKSN"
     gene            2772..5741
                     /locus_tag="PLL28_05275"
     CDS             2772..5741
                     /locus_tag="PLL28_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNF68766.1"
                     /translation="MLTARLVIISLLTAGMVSAAKRPAIPMPVDDTKVPYYEKDYPWV
                     DSLMQVMTLDQKIGQLFMAPAYSNKDEAHAKSIDNLVTKYQIGGLIFMQGGPVRQAKL
                     NNRYQKESKIPLLIGMDAEWGPAMRLDSVINFQRQLTWGAMSNDSIVYEVGTVIAQQL
                     KRLGVHVNFAPDIDINNNPNNPVIGDRSFGENKYNVALKGLMYMNALQDNHIVACGKH
                     FPGHGDVDADSHLTLPVVNHPYNHLDTVEFFPFKVLMNEGLGSIMLAHLYVPALDSTK
                     NQPSSLSPKVGRDLVRERLGFKGLIFSDALNMKGASAYYEDGELEVKAFLAGNDMLLF
                     SDNIPVAFEAIKKAVASGRITQAQIDESVKRILIAKAFAGLNVSKNKWVAEKNITDDI
                     NKPAATLIKRKITENSITLANAADTLIPFKKLDTLSIACISVSGGSKTTFQEYLAKYA
                     KVDLYQINKDADATAFVQSFEKLKNYDVVIVGVHDMKRARTSNYGITSNTENLVWKLS
                     RATKTVVCMFGTPYAIDKFSDAQYQIVSYDDDNYTQQATAMALFGAIPFHGRLPVGMG
                     KFKVNAGIETGSLQRIKYTIPEEVGIIPARLNRIDSLAKACIKVEAAPGCQVLVLKDG
                     NVIYDKAFGSYKYDNKDKVDVSSIYDLASVTKTSATTIMLMDLHDKGLLNLQKTVSDY
                     LPETEGTNIAGLKMQDILTHQAGLPAWVPFYKRTLNPDGTINPLYYSQTKIPGFTTQV
                     ADGIYMRDDYKDSVWNIIEHVDIKEKKYLYSDLSMYIGRRVAERIANVPLDVYLYQHF
                     YGPLGMQTTCFNPLQRFPKARIVPTENDNYFRYQTVQGYVHDMGAAMMGGVEGHAGLF
                     SDAEDLAILYQMLLNKGYYGGTQYFSPETVALWTKKQSSTSRRGLGFDKPEMNGPSPC
                     SGYASASAFGHQGFTGICVWVDPEYNLVYLFLSNRVQPKAEPNRLSTEGIRNKIMDVI
                     YEAILTGNPPKGSPASD"
     gene            5873..7012
                     /gene="bshA"
                     /locus_tag="PLL28_05280"
     CDS             5873..7012
                     /gene="bshA"
                     /locus_tag="PLL28_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HNF68767.1"
                     /translation="MKIGIVCYPTYGGSGVIATELGKALADKGYKVHFITYKQPFRLD
                     SFHENIFFHEVNFTNYPLFEYPPYEIALSSKIVDVAINEKLDLLHVHYAIPHASAAYL
                     AKQILKDKNYHLPFITTLHGTDITLVGKDETFVPVVTFSINQSDGITSVSDSLKLDTY
                     QNFDIEKEIAVIPNFIDFTRFKKLNKEHFRKVIAKNGEKIVVHTSNFRKVKRVEDVVK
                     VFCKIKEAVPSKLLLIGDGPERQNVEKLARQVCLEEDVYFLGKQEAIEELLAIADLFI
                     LPSETESFGLAALEAMACEVPVISSNTGGIPEINIQGVTGFLSNVGDVEDMAKNAITI
                     LSNEDTLKQFRANAIKQAQAFSLENILPQYEAYYRKVVEESKSAR"
     gene            complement(7009..7422)
                     /locus_tag="PLL28_05285"
     CDS             complement(7009..7422)
                     /locus_tag="PLL28_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68768.1"
                     /translation="MKYFLPFLILLSGCSILGSHKSVYTPLNGDWYPIDQKFAGLQFP
                     ESTYATQRLMLRDSSYILSSEVVDSGKMICSDSTMDIYGIEGPNAGNHFMAIYKLRND
                     TLTISYNLTGGSYPDTFVSDSTNYYFVSIFVRAKD"
     gene            7546..7998
                     /locus_tag="PLL28_05290"
     CDS             7546..7998
                     /locus_tag="PLL28_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNF68769.1"
                     /translation="MSLFDQVNEGIKDAMKSKQEGRLRALRGIKAALLLAKTEKGAED
                     TLTEAKEMQVLTKMMKQREESYATYTQQNRPDLAAAEKEEMDVIAAFLPKQMSAEELR
                     AEVAAIITQLGASGMKDMGRVMGTATKQLAGKTDGDKIAAVVKEILGA"
     gene            8040..8582
                     /locus_tag="PLL28_05295"
     CDS             8040..8582
                     /locus_tag="PLL28_05295"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNF68770.1"
                     /translation="MILDIIYAIILLLAMIHGYRKGIIHSIVSLLALIVGLLAAVRFS
                     ELAAVYIDKWFNISGKYLPLIAFIAVFIGLYLLFRLLEQALEGFFKLIKLNFVNQIIG
                     ALIWGLVWTMLFSTILFYLNNMGLFSDELKSDSVTYEKIAPIAPDTFELIGKIIPPVK
                     NTYNSLDEWFHKLHDNATSD"
     gene            8588..9157
                     /locus_tag="PLL28_05300"
     CDS             8588..9157
                     /locus_tag="PLL28_05300"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNF68771.1"
                     /translation="MKILLLDNYDSFTWNLYDYLTRCGANVHVYRNDAITPQVISDMS
                     FNGIVLSPGPGRPQDAGIMMELIEKFHQTHPILGICLGYQALGAYFGAEVIHAPLPMH
                     GKTSTISHDGKGLFEGVPNPTSVMRYHSLNIDRMPPGIRLTAQTADTGEPMALAHEYL
                     PLSGVQFHPESVLTPAGLTMIGNWLKSMH"
     gene            9203..9961
                     /locus_tag="PLL28_05305"
     CDS             9203..9961
                     /locus_tag="PLL28_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF68772.1"
                     /translation="MTHTVTEENGFKYVEEGEGEVLMLLHGLFGALSNFFDIIEHFKA
                     THKVVIPILPLYELPIEETTVGGYVDYVERFVAYKGYGKVIVLGNSLGGHIALIYTLQ
                     NMEKVKAMVLTGSSGLFESAMGDSYPKRGDYEYIKRKTEVTFYDTKHATKELVDELFD
                     IVNDRNKVIRVLATAKSAIRHNLAEEVSKITVPTLLIWGNNDTITPPFVGEEFHKLLP
                     NSQLYFIDKCGHAPMMEQPEEFNRYLDKFLKEIN"
     gene            9963..10628
                     /locus_tag="PLL28_05310"
     CDS             9963..10628
                     /locus_tag="PLL28_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNF68773.1"
                     /translation="MLAKDIISENIPPLKVKDTGTKAIEWMYEFKLSHLPLVDNKKYI
                     GLVSEEDILDFNNTNEQLGKFLRNLYKPFVRDTEHIYEVLRVAATLKSTVIPVIDAEE
                     TYLGLITLQSLLYHFAKMSSISEPGGVIILELNQKTDYVLSDIARMVESNDAHILSLY
                     FNVDPDTGKHAVTIKVDTTEVKHIVATFERYEYQVRAYFRESDLGDIMKDRYDSLMNY
                     LNI"
     gene            10630..11514
                     /locus_tag="PLL28_05315"
     CDS             10630..11514
                     /locus_tag="PLL28_05315"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HNF68774.1"
                     /translation="MHIAIYGRLIKKDALPYVQQMFNVLHERNIHVYIHESYFPHLVN
                     NIQFKSEPVLFNEKNMAVIARIDYIFSVGGDGTLLDTITIVRDTNIPIVGINIGRLGF
                     LANIGKDEIISLINALETDAVTRDVRTLVHLDSNRPDLFEGDAFALNEFTIHKKDSSA
                     MIVIHTYINGEFLMSYWADGLIVSTPTGSTGYSLSCGGPIIFPTSENFVITPVAPHNL
                     NIRPIVVSDSSVISFEIEGRSDQFLVTLDSRYESVDASVQLAVRKENFTVTLVRPHEQ
                     NFLDTLRNKLSLGLDQRN"
     gene            11587..12438
                     /locus_tag="PLL28_05320"
     CDS             11587..12438
                     /locus_tag="PLL28_05320"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HNF68775.1"
                     /translation="MKNTFALLVFIQITLSGFSQITNPDPYCSGTFDGGISGAMMKYI
                     SKVEIDEVSWQHVSGNANPSPGYTYFNNIEAPVVQQGNTYTFTVTHSGIAIHFVTVFI
                     DFNGNNTFDVAEGEAFFETANTPEFEFPTSPTTFSITIPNDALNGTTRMRVQVFEDDD
                     YTWNLFNNEPPPCTAYAAGQMLDYGETEDYDLVIGGETIAIQPGSIATDLIVSPNPAS
                     DYWSIQLASDAVYHIDLYSINVEKLISYTSSLPLSIPATDLPSGAYILHLSDDKGRFS
                     YVHLLKH"
     gene            12575..13432
                     /locus_tag="PLL28_05325"
     CDS             12575..13432
                     /locus_tag="PLL28_05325"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HNF68776.1"
                     /translation="MRSLFIYVSVLAVIVTVPNRSSAQAAEIGLWGGLTHSFGDINNS
                     TESFQFLEPGAGVFFRYNLNSRIGYYLGLSGGQTRGYDSISNNIYQLRRNLSYRTTIY
                     DVSGRVDFNFLPLEREKPKYWFTPYVFMGLSVYYFNPQAYYNDEWVDLQPLGTEGQQF
                     PELTGNDKYQRVQISVPMGGGFKFALNKNITAGIEWNWHWLFTDYLDDVSTVYVDPSI
                     LASGSDGALAVALADRSTAIDIIPLGEPGKQRGDSEHRDKYLYTGIFLSYTIVDLKCD
                     PPGKWGAKF"
     gene            13450..14193
                     /locus_tag="PLL28_05330"
     CDS             13450..14193
                     /locus_tag="PLL28_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNF68777.1"
                     /translation="MSAGQPDPNNIPRHIVIIMDGNGRWAKLRDKARIYGHHAGVEAV
                     RKVVEACTELGVEYLTLYAFSTENWKRPEEEVNALMELLVRTIRRETPELDKNNVRIG
                     MIGDGHSLPPACIAELEEAKKMTADNTGLNLILALSYSGRWEITHAVQQIAKELVNGK
                     IKPEEITEQTIADKLNTAAMPEPELLIRTSGEYRLSNFLLWQTAYTEYYFTETLWPDF
                     DKEALFNAIIDYQNRERRFGKTTEQIQVK"
     gene            14190..16874
                     /locus_tag="PLL28_05335"
     CDS             14190..16874
                     /locus_tag="PLL28_05335"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNF68778.1"
                     /translation="MKRTLLSLLLCGVMIATLSVTGWSQTPGLDSNSVDYGNPKKYTI
                     AATEIRGVTSPNLDLNSLIVRTGLTPGLPLTIPGEDFSVAIKSLWKMRLFSDVQIFVE
                     KTLGDQVWLTIQVTELPKVSKYAFTGTTKSEDEDLRPQLDIIKNVTPFADYTKTHIYN
                     TVREYYAEKGFLNAEIDVYGKPDTSFRNSVIVYIDVVKNAKVKIATIDIHGNTEMSDR
                     EVLKNMKETRVRTQIKPFYNDPENPISDDVLSVKGVVNVLSSLSVSNIMDFVYDRAQI
                     RIFNQSKYDEAKARTDAQAVITYYKSKGYRDAQMKLDSVYNVSDRSIGLSYTIDEGTK
                     YYFRNITWKGNTKFTDKQLDSILDISKGQVYNQSYLDQRISFDLTKPDITSKYMDDGY
                     LFFRINPVEIWAQDDSIDLEIRISEGPQAIIDKIIISGNTKTNEHVIRRELRTLPGQK
                     FNRSLVIRSQREIIALGLFDAEQIGINPIPHPESGTVDIEYTVVEKPSDQLELSAGYG
                     GQGYGVLASVGIVLNNWSTTQMFKKEGWKPVPMGDGQKLSFRVNTTGRLYQAYNIGFV
                     EPWFRGKKPNSLSFSLTRTQLGYNIPGSTYDSLEGKFIANGASLGYGIRLKWPDDYFT
                     LVSTVSFYNYSLTDYPYFIATNGDYNNFSIKETLGRNSLDDQQFPKSGSSISLSLQFT
                     PPYSALNSKDYGELGTEEKYKFIEYYKWRFNADWYTPVVGKFVFRASMKFGFLGYYNS
                     DVGIPPFERFQLGGDGLSGGYTNTFVGTDIISMRGYEVFPQTNSSSARTEAIFNKYTL
                     ELRYPIVKSQSANIYALAFAEGGNLYADIDNYNPFEIKRAAGLGVRAWLPMFGLLGVD
                     YGIRFDDLVPGDLEQASGFFDYITRNGKFTVVLGFEPE"
     gene            16890..17417
                     /locus_tag="PLL28_05340"
     CDS             16890..17417
                     /locus_tag="PLL28_05340"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNF68779.1"
                     /translation="MKKQLILATIVMAFAVTAQAQRFAYVNTDYILDNMPEYKKAQEQ
                     IDKLTQEWRGEADKKQKEIDELYRSFQNEQYLLTEDQKKAKISEIETKEKALKDYQKS
                     VFGYEGLLYQKRQELVKPIQDKVYEAIEKYARERGYDFIFDKSSSTTILWANPDNDKS
                     DEIIKKLGYTPTKTN"
     gene            17453..17953
                     /locus_tag="PLL28_05345"
     CDS             17453..17953
                     /locus_tag="PLL28_05345"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNF68780.1"
                     /translation="MKNKLMLLFAIAVMAISQADAQKIGYLNTNELLVQMPEVKKADS
                     VLNQYAEEMQKIYAAYVMEYQQKLADYQNNAASWSEVKRESVEKDLGNLQIRISDFEK
                     ESNDKLEAKKEELYGPILDNVKAKIKLVGDENKFTTILDGSAMLYVGIDAVDILPLVK
                     KKLGLQ"
     gene            18054..19136
                     /locus_tag="PLL28_05350"
     CDS             18054..19136
                     /locus_tag="PLL28_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68781.1"
                     /translation="MKRILTLVLVLTFSVHAYSQSKKDQALEKALTAIAMMDSGYITE
                     SIDLLNEAIKLDPDRYDYPYEVAYAYYLMKDYNKSIEVLEKLSKHKDVTDQLYQLLGN
                     SYDIIGDPDKAFDAYDRGLKKFPKSGILFLEKGNVYWGQKKYAEALPFYEDGIAADPM
                     FSSNYYRAARIYCATDNPFFGIMYGEIFLNLEPSTERSQEISALLFDTYKSNIHVTSD
                     TSASIEFCSNNLVLDKESMKRMSEGILPFCMTAETTMLIYVGFSKEITLESVCKLRSS
                     FIDAWYQMGNDKKYNIELFKFQKDLADKGYYDAYNHWLFMNGDGAAFDEWYKTHQDEF
                     TNFANYANQHRLQLNDKTKFLVADFR"
     gene            19182..19997
                     /gene="murI"
                     /locus_tag="PLL28_05355"
     CDS             19182..19997
                     /gene="murI"
                     /locus_tag="PLL28_05355"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNF68782.1"
                     /translation="MFPIGVFDSGYGGLTVLRSFKKALPEYDFIYLGDNARTPYGNRS
                     FETVYEYTLECVNHLFNMGCRLVILACNTASAKALRTIQQNDLPKIDSSRRVLGVIRP
                     TAEIIGKMTHTGTVGVFATAGTVQSESYIIEIAKAWPKITVVQEACPMWVPLIENNEY
                     NKPGADYFVQQHMLQLLQKAPELDTILLGCTHYPLLIEKIKQFAPQGTTIIAQGDIVA
                     ESLVDYLKRHPEMESQCSKNGTMQFYTTDDTANFNAHASAFFGSEVQSTFLHL"
     gene            complement(19986..>20412)
                     /locus_tag="PLL28_05360"
     CDS             complement(19986..>20412)
                     /locus_tag="PLL28_05360"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF68783.1"
                     /translation="TQGRLLPHQFILIQGISDEHRDEQLTDNIRVISHLPGKELYDLI
                     LRCDVIISRGGYSTLMDLALLNKKCIFVPTPGQTEQEYLVKELGEKGLVVAGDQSTFR
                     LEDALEKVQHTKGFYIPSNEQTFIPFLEAAIAEAVKSKV"
BASE COUNT         6199 a         4257 c         4253 g         5703 t
ORIGIN      
        1 ccattgatat tttatatcat cgaaaaatgc catctatgca ccaagggtga ttaatagaaa
       61 cgccttgaat ttttttgcca gtatttgatc attataaatc caatcaatgc gccacctaaa
      121 tgtgcaaagt gcgcaacatt gtcgttagga tcattgatga aaccggtata taattcgtag
      181 gctatcaaaa tgatgacaag atatttcgcc ttgatcggaa gaaggaaata aatataaatg
      241 agcgtattgg ggaataacaa gccaaaacct accaggatac catagatggc tccggatgcc
      301 cctaccgcac tgtagttcaa gccatttaca ttccagcgca aaggcgtatc agtaatggat
      361 gaagaaggga aaatggtgcc gactgattga tatacttcat aggcatgata cgcagtaaaa
      421 cataatgctg cacctaaacc ggtaattaaa aaatattgca aaaatcgttt tggaccccac
      481 acattttcta atactgcccc aaacatccac aaactgaaca tgttgaaaaa cacatgcgca
      541 aagttttcat gggtaaacat atgcgtcacg atttgatatg gcctgaattc cggtgtcgac
      601 ggatagtgca acgcaagcgt ttccttcaat tccggaaatg taaaagttgc aagatatacc
      661 aacccattaa tgatcaatag atttttgatc accggcggca gataattgaa ccttgtaaat
      721 tgtatttgcg acatgttatt gtttaattgc ttttttgaaa ttgtcgtgcc agtgcttcaa
      781 gggtaaaggt gacaaaagtg agattgccaa aaggagaagt gtatggcgtt tcacaggcaa
      841 ataattgatc tgctaaatga cgcatgcccc gctcgtccag tttttgtccg cgcttgatgc
      901 tgtttgtacg tgcaagtgat cttgcaatag cttcatggcg atcgagcttg agtgttgatt
      961 tatcctcttt aaactgctct aacaatgctt ccactacgcc attgatatcg ccgttttcaa
     1021 attcaaccgg tatgccatgc actacaaaag cgtctttgcc aaagggcatg atgtcgaagc
     1081 ccaggttttg cagatcagga agcaacattt ccatcaacgc aaaatcggca cccgataatt
     1141 ttaatgtttc aggaaataat tgctgttgcg agtgatgctt tttctctctc aattgcgtaa
     1201 catacttttc atataaaatg cgctcatgtg ccgcttgctg atcaattaca ataaatcccg
     1261 actttatttg agtcactaca tattgataat gtatttgaaa tggtgtagcg ccatcggttg
     1321 gcatatccga ttgcacctca tcatcatgca atgcacccga aacttctcgt ttaaatgctg
     1381 tatgcatgcg gttggttgat gccacatcat gtttaaatga cgattgatgc tgcggaatat
     1441 cgttcatcct gctctcaaca gttttgaaac tcaccggctg ctcctcaggc tttttgtaga
     1501 aacttggcag attcgcaaac gtttgatcct gctcgaaatc aagtgtcggc gttacgctat
     1561 attgtgccaa ggcatgtcgc accgccgctt gaatgaaagt atatataatg cgctcgtcct
     1621 caaattttat ttcctgtttg gtaggatgca cattcacgtc gatgcgcgcc ggatcgatat
     1681 caatgtaaat ggtatacaat ggataacttc cctcgggtaa caactcctgg tatgccgaca
     1741 ttacggcatg atgtaaatac gggcttcgaa taaatcggtt gttgacaaaa aagaattgtt
     1801 ctcccttggt tttttttgct gcatcgggtt tgccaatgaa accacggata ttagtaactg
     1861 aggtgtcttc tgaaattggc accagcttct cattgtaatt tgaaccaaac aaatgaatga
     1921 tgcgctgacg tagatttcct gccttcagat gaaataattc tactccatta tgctgcagac
     1981 tgaagaaaat atcaggatgt gctaaaacaa cacgctcaaa ctcatcaata atatgtttaa
     2041 gttcaacggt gttcgatttc aaaaagttgc gcctggcggg aacattataa aaaaggtttt
     2101 tcacctgaat atttgttcca accggacatt gagcttgctc atgtgcctta acctcaaaac
     2161 cttcaatcac taaccttgca cctaattcat gctcttgctg acgagttttt agttctacct
     2221 gggctatcgc cgcgatactg gcaagcgctt cgcctcgaaa tcccttcgtg cgaatagaaa
     2281 aaatgtcatc ggcactgcgg atcttactgg tggcatgcct ttcaaagcac atcctggcat
     2341 cggtaaaact catacccttg ccattgtcta tcacctgaat aagcattttt cctgcttcct
     2401 tcacaacaac cttgatctcc gttgctcctg cgtctacggc gttttccatc agctccttga
     2461 tagccgaagc agggcgttga attacctcac ctgcagcaat ctgattggca attgaatccg
     2521 gtaaaagatg aataatgtct cccatggcta attcactcca gacgcgcagc tcacaagtcc
     2581 actctcccgc ctgaattgca aaagtaatgt tttacgggtg gctaatgtct gaattagtat
     2641 gctatttctg cgcttgacca caacattagg gtaggaatta tcgttccagc ggttaccttg
     2701 accattattt ttgcaaggaa agtgaaaaca aggcatactt ttggtttcgt attgcccgaa
     2761 agcattcaag catgttaaca gcacgactcg tcataatatc gcttctcacc gccggaatgg
     2821 tgtctgccgc aaaacggccc gccataccca tgcctgttga tgataccaaa gtgccttatt
     2881 acgaaaaaga ctacccttgg gtcgattccc tgatgcaggt aatgaccttg gaccagaaaa
     2941 ttggtcagct ttttatggca cctgcctatt caaataagga tgaagcgcat gcaaaatcta
     3001 tcgacaacct ggtaacaaaa tatcagatcg gcggactcat ctttatgcag ggcgggccgg
     3061 tccgtcaggc aaaattgaat aacaggtatc agaaagagtc gaaaatacca cttctgatag
     3121 gtatggacgc cgaatgggga cctgccatgc gcctcgattc tgtcatcaac ttccagcgcc
     3181 agctcacctg gggcgccatg agcaacgata gtatcgtgta tgaagtggga accgttattg
     3241 cccaacaact aaaaagactt ggtgtgcacg tcaatttcgc accggatatc gatatcaata
     3301 ataatcctaa taaccctgta attggcgata ggtcgtttgg agaaaataaa tacaatgtgg
     3361 cgcttaaggg cctgatgtac atgaatgccc ttcaagacaa tcatattgtt gcctgcggaa
     3421 aacattttcc ggggcatggt gatgttgatg cggatagcca tcttacattg cctgttgtaa
     3481 atcatccata taaccatctg gataccgttg aatttttccc tttcaaagtc ttgatgaatg
     3541 aagggctcgg atctatcatg ttggcacatt tatatgttcc cgcactggat agtaccaaaa
     3601 atcaaccttc atccctctcg ccaaaagttg gtcgcgatct ggtgcgcgaa cgtctcgggt
     3661 tcaagggtct catattcagt gatgcgctta atatgaaagg tgccagtgct tattatgaag
     3721 atggcgaatt agaagtaaaa gcattccttg ccggcaacga catgttgttg ttcagcgata
     3781 atattcccgt tgcttttgag gcgataaaaa aagcagtggc atcgggaaga attactcagg
     3841 cacaaattga tgaaagcgta aaacgcatcc tcatcgcaaa agccttcgca ggattaaatg
     3901 tcagcaaaaa taaatgggtg gcggaaaaaa atatcaccga tgatattaat aaaccggctg
     3961 ctacattgat taaaagaaaa attactgaaa atagtattac acttgcaaat gctgcggata
     4021 cattaatacc atttaaaaaa cttgatacat tatctattgc atgtatcagt gtttccggtg
     4081 gaagtaaaac aacctttcag gaatatcttg ctaagtatgc gaaggtagat ttatatcaaa
     4141 taaataaaga tgcagatgca acagcatttg tgcagtcgtt cgagaagttg aaaaattatg
     4201 atgtagttat tgtgggtgtt catgacatga aaagagcgcg aacttctaac tacggcatta
     4261 cctcaaatac agaaaacctg gtatggaaat tatcgcgtgc tacaaaaaca gtagtatgta
     4321 tgtttggcac accatatgcc attgataaat tcagtgatgc acaataccaa attgtatcct
     4381 acgacgacga caactatacc cagcaagcta ctgctatggc gctttttggc gcaattcctt
     4441 tccatgggag attacctgtg ggcatgggaa aatttaaggt aaacgcagga atagaaacgg
     4501 gctcacttca acgtattaaa tataccatac ccgaagaagt tggtatcatt cctgccagat
     4561 taaacagaat cgatagcctt gcaaaagcat gtattaaagt tgaagcggct cccggatgtc
     4621 aggtgcttgt tttgaaagat ggcaatgtta tctacgataa agcattcggt agttataaat
     4681 atgataataa agataaagtt gatgtaagca gtatttatga cctcgccagt gttaccaaaa
     4741 catcagcaac aaccatcatg ctcatggatc tgcatgataa aggtttgttg aatttgcaga
     4801 aaacagtttc tgattattta cccgaaacag aaggaacaaa tattgccggt ttaaaaatgc
     4861 aggatatcct tacgcatcag gccggattac ctgcttgggt gccattttat aaacgcacac
     4921 ttaatcccga tggcactata aatccattgt attatagtca aaccaaaatc cccggattca
     4981 ccacacaagt tgcagatggt atttatatgc gcgacgatta taaagacagc gtttggaata
     5041 ttattgagca cgtagatatc aaagaaaaaa aatatttata cagcgacctg agtatgtata
     5101 ttggtcgacg ggtagctgaa cgtattgcga atgtaccatt agatgtttat ttgtatcagc
     5161 atttttatgg tcccttggga atgcaaacta cttgctttaa tcccttgcag cgtttcccca
     5221 aagcgcgcat tgttcctact gaaaatgata attatttccg ctaccagacc gtgcaaggat
     5281 atgtgcatga tatgggcgct gccatgatgg gaggggttga aggtcatgcc ggtttattca
     5341 gcgatgccga agacctggcc atattgtatc agatgctgct caacaaaggt tattatggcg
     5401 gcacccaata tttttctccc gaaacagttg ccttatggac taagaagcaa agtagcacgt
     5461 ctcgtcgcgg acttggcttc gataaaccgg aaatgaacgg accatcacca tgcagtgggt
     5521 atgcctctgc ttctgctttt ggccaccagg gatttaccgg aatttgtgtg tgggtggatc
     5581 ctgaatacaa tctcgtttat ctattccttt ccaatcgcgt tcaaccaaag gcagagccca
     5641 accgtctgag cactgagggt attcgaaata agatcatgga cgtgatctac gaggcaattc
     5701 tcacgggaaa tcctcccaaa ggaagcccgg cttctgattg aatttctgta gccacttgtg
     5761 tatgattgaa cactgatttc tccattgggt tgcttggcaa tcgtggcctt atttgtcccg
     5821 cgttgaactt ttccggctaa attttcattc tacttctcat taccttcaca acatgaaaat
     5881 tgggatagtt tgttacccca catatggggg aagtggtgtt atagcaacgg aattagggaa
     5941 agcactggca gacaaaggct ataaagtgca cttcattacc tataagcaac cctttcgttt
     6001 ggattccttt catgaaaata tttttttcca tgaagtgaat tttactaatt atcccttgtt
     6061 tgaataccca ccctatgaaa ttgcactaag tagcaaaatc gtggacgtgg ctatcaatga
     6121 aaaactcgac ctcttgcacg tgcattatgc tattcctcac gcatcagctg cttatttggc
     6181 aaagcaaatt ttaaaggata aaaattatca tctgccattt attactactt tgcatggtac
     6241 tgatatcaca cttgtaggaa aagatgaaac ctttgttcct gtagttactt tttccatcaa
     6301 ccaaagcgat ggcattacgt ccgtttctga tagtttgaaa ttagatactt atcaaaactt
     6361 tgatatcgaa aaagaaattg ctgtcattcc aaactttatt gattttacac gcttcaagaa
     6421 actcaataaa gagcatttta gaaaagtgat tgcaaaaaat ggtgagaaaa tagtggtaca
     6481 tacttctaac ttccgaaaag tgaaaagggt agaggatgta gtgaaagtat tttgtaaaat
     6541 aaaagaagca gttccttcaa aattactcct catcggcgat ggacctgagc gtcaaaatgt
     6601 agagaaactc gctcgtcagg tatgccttga agaggatgtg tactttttag gtaagcaaga
     6661 agccatagaa gaattattgg ccattgccga tttatttatt cttccatcag aaaccgaaag
     6721 tttcggtctg gctgccctcg aagcaatggc atgtgaagtg ccggtgatat ccagcaatac
     6781 aggtggtatt ccggaaatta atattcaagg tgttacaggt tttctcagca atgttggcga
     6841 tgtagaggat atggcgaaaa atgccattac catattgtca aacgaagaca ctttaaagca
     6901 atttcgtgcg aatgcaataa aacaggctca ggctttttca cttgaaaata ttctgccgca
     6961 atacgaagcc tattatcgca aggtggttga ggaaagtaaa tctgctcgct aatccttggc
     7021 gcgaacaaat atcgaaacga aataatagtt cgtcgaatct gaaacaaatg tatcaggata
     7081 cgaccctcct gtcaaattat agctgatggt caatgtatca ttgcggagtt tataaatcgc
     7141 cataaaatga ttacctgcat ttggtccttc aataccataa atgtccatgg tgctgtcgct
     7201 acagatcatt tttcccgaat caacaacttc cgacgataaa atataactcg aatcacgcaa
     7261 catcaatcgc tgcgtggcat aagttgattc cggaaattgt aatccggcaa atttttgatc
     7321 tatcggatac cagtcgccat tcaacggcgt ataaactgat ttatgacttc ctaaaatcga
     7381 acatccggat aacaaaatca gaaatggtaa aaaatatttc atatcactaa attttcgggg
     7441 aatattctat ttaaaacaat tttgttgatg tgggcacgaa ggtatactat tttctaaaat
     7501 tgagcttgtc actttttcat gtaactttac actaaaataa aaaatatgtc acttttcgat
     7561 caggtaaacg aaggcattaa agatgctatg aaaagtaaac aggaaggacg cctgagggca
     7621 cttcgcggaa ttaaagcggc gctcttattg gcaaaaactg aaaaaggtgc ggaagacacc
     7681 ctcacagagg ccaaagaaat gcaggtcctc actaaaatga tgaagcaacg cgaagaaagt
     7741 tatgccacct atacacaaca aaacagaccg gatcttgcag ctgcagaaaa ggaagagatg
     7801 gatgtgattg ctgctttcct gccaaaacaa atgtcggctg aagaattgcg tgcggaagtt
     7861 gcagccatca tcacacaatt gggcgcaagt ggcatgaaag atatgggtcg tgttatgggc
     7921 actgcaacca aacaacttgc aggcaaaacc gatggcgata aaattgccgc cgtagtgaaa
     7981 gaaatccttg gtgcctaagt gcaggcattt gtttttattt caaaaaataa gcgctcaatt
     8041 tgatactgga tattatttac gcaataatcc ttttgttggc catgattcat ggatacagaa
     8101 aaggtattat tcattctata gtatcgctgt tagcacttat tgtcggatta cttgccgccg
     8161 tccgatttag cgaactggca gcggtatata ttgataagtg gttcaatatt tccgggaaat
     8221 atctgccact gatcgctttt atcgccgttt ttatagggtt atatctatta ttcagattgc
     8281 tcgaacaagc acttgaaggc ttttttaagc ttatcaaact gaattttgta aaccagataa
     8341 ttggggcatt gatttgggga ttagtatgga ctatgctttt ctctaccata ttattttacc
     8401 tgaataacat gggactgttt tccgatgaat tgaaatcaga ttccgtcacc tacgaaaaaa
     8461 ttgctccgat tgctccggat acttttgagt tgatagggaa aattattccg ccggtaaaaa
     8521 atacctacaa cagtctcgac gagtggtttc ataaacttca tgataacgca acaagcgatt
     8581 aacatgcatg aaaatactgc tgctcgataa ctacgattcg tttacctgga atctttacga
     8641 ttacttgacc cgctgtggtg ccaatgtcca tgtatatcga aatgatgcca ttaccccaca
     8701 ggtcataagt gatatgtcat tcaacggcat agtgttgtct ccgggtcccg gaaggcctca
     8761 ggatgccgga attatgatgg aattgatcga aaaatttcac caaacccacc caattttagg
     8821 tatttgcctc ggttatcagg ccctgggagc ctattttggt gccgaggtaa tccacgcccc
     8881 tcttcccatg catggaaaaa cctcaaccat atcgcacgat ggaaaagggc tctttgaagg
     8941 cgtgccaaac cccacgtccg taatgcgcta tcactccctg aatatcgacc gaatgcctcc
     9001 ggggataagg cttacagctc aaactgccga taccggtgaa cccatggctt tggcacatga
     9061 gtacctgcct cttagcggtg ttcagttcca ccctgaatcg gtgttgacac ccgccggact
     9121 aaccatgatc ggcaactggc tgaaaagcat gcattagcaa acctgacaat aaatgctatt
     9181 tttgtaacgc actcaacacg gtatgacgca cactgtcaca gaagaaaacg ggtttaaata
     9241 tgtggaagaa ggcgaaggtg aagtactgat gcttctgcac ggcctattcg gcgccctcag
     9301 taattttttc gacatcatcg aacactttaa agccacgcat aaggtggtga tacccatatt
     9361 gccactatac gaattgccta tcgaagaaac tacagttggc ggttatgtcg actatgtaga
     9421 gcgcttcgtt gcctacaaag gttatggcaa agtgattgta cttggaaatt cactcggagg
     9481 ccatatcgcc ttgatctata cactccagaa tatggaaaag gtaaaagcta tggtgctcac
     9541 cggtagctcc gggctttttg aaagtgccat gggcgacagc tatccgaaac gcggtgatta
     9601 tgaatatatc aaacgcaaaa cagaggttac tttctacgat acaaaacacg ctaccaaaga
     9661 actggttgat gagctttttg atatcgtgaa cgaccgcaat aaagtaatcc gggtactggc
     9721 aacagcgaaa agcgccatcc gccacaacct ggctgaggaa gtatcaaaaa ttaccgtacc
     9781 taccttgctc atctggggta ataacgacac cattacacct cctttcgttg gtgaggagtt
     9841 ccataaactt ttaccgaatt cacaacttta tttcatagat aagtgcggac atgcaccaat
     9901 gatggaacaa ccggaggaat ttaatcgtta tctggataag ttccttaagg aaataaatta
     9961 agttgctcgc aaaagatatc atatcggaga atataccgcc cctgaaggta aaagacaccg
    10021 gcaccaaggc gatagaatgg atgtacgaat tcaaattgtc acacctcccg cttgtcgaca
    10081 ataaaaaata tatcggtctc gtaagcgaag aagatatcct cgattttaac aatacaaacg
    10141 agcaactcgg aaaattcctg cgcaatcttt acaaaccctt tgttagagat accgaacata
    10201 tttatgaagt gcttcgcgtt gcggcaacgt taaaatcaac agtaattcct gttatcgatg
    10261 ccgaagaaac ctatcttgga ttaatcactt tacaaagttt gttataccac ttcgcaaaaa
    10321 tgagttccat cagtgaaccc ggcggagtga tcatcctcga attaaatcaa aaaacagatt
    10381 atgtgctcag cgatatcgcc cgcatggtgg aaagcaacga cgcccatatt ctgagcttat
    10441 attttaatgt tgatccggat accggaaaac atgccgtaac cattaaggtt gataccactg
    10501 aagtgaaaca tatcgtagct actttcgaac gttacgaata tcaggtgcgt gcctatttca
    10561 gagaaagtga tctgggtgat atcatgaaag accgctacga ttcattaatg aattatctca
    10621 atatctgata tgcacatagc catctacggt cgcctgataa aaaaagatgc gctgccttat
    10681 gtgcaacaga tgtttaatgt gttgcatgaa cgcaatattc atgtatatat acatgagtcc
    10741 tattttccgc atctggtgaa taatatccaa ttcaaaagcg aaccggtttt attcaacgaa
    10801 aaaaatatgg ccgtcatcgc gcgcatcgat tacatcttta gcgttggtgg cgatggcaca
    10861 ctgctggata ctatcaccat cgttcgcgat acaaatattc ccattgtcgg aattaatatc
    10921 ggtcgattgg gatttcttgc caatatcggc aaagacgaaa ttatctcttt gatcaacgca
    10981 ctcgaaacag atgccgttac tcgcgatgtg agaacactcg tgcacctcga ctccaacaga
    11041 ccggatctct ttgaaggtga tgcattcgca ttgaatgaat ttaccattca taaaaaggat
    11101 agctctgcca tgattgtcat acatacttac atcaatggcg aattcctcat gagctattgg
    11161 gccgatggat tgatcgtttc aacaccaacc ggttctaccg gatattcatt gagctgcgga
    11221 ggcccgatta ttttcccgac atccgagaat tttgtgataa caccggttgc tccgcataac
    11281 ttaaatatac gtcccattgt agtaagcgac agctctgtta tcagcttcga aattgaaggt
    11341 cgcagcgatc aatttttagt caccctcgat agtcgctacg aatctgttga cgcttcagtg
    11401 caattggcgg tacgcaaaga aaactttacc gttaccctcg tgcgacctca tgaacagaac
    11461 ttcctcgaca cactgcgcaa taaactttcg cttggtttag atcagcgcaa ttgaattaga
    11521 tttttggtgt aagtaattat gcatttatcg cgatttatta ttgcgcattg tcaataacat
    11581 tgcgaaatga aaaatacttt cgcgctcctc gttttcatac aaataacact ttcgggtttt
    11641 agtcaaatta ccaaccccga tccatattgc agcggtacat ttgatggtgg tatcagcggc
    11701 gccatgatga aatatatttc caaggtggaa attgatgagg taagctggca acatgtttcc
    11761 ggtaatgcaa acccctctcc gggttatacc tattttaata atatcgaagc accggttgtg
    11821 cagcagggaa atacctatac ttttactgtc acccattccg gaatagccat tcactttgtg
    11881 acggtattta ttgattttaa tggaaataat acttttgatg ttgctgaagg tgaagcattt
    11941 tttgaaacag caaatacccc tgaatttgaa ttccctactt caccaactac attttccatc
    12001 accataccca atgatgccct taacgggaca acaagaatgc gtgttcaagt gtttgaagat
    12061 gatgactata cctggaattt attcaacaat gaacctccgc catgcacagc atatgccgcc
    12121 ggtcaaatgt tagattacgg tgaaactgag gattatgacc tcgtaattgg aggtgaaaca
    12181 attgccattc aaccgggatc cattgccacc gatttaattg tttcacctaa cccggcatct
    12241 gactattgga gtattcaatt ggcatcagat gccgtatacc atatcgacct ttactccata
    12301 aatgttgaga agctgatttc ctatacatca agcctaccac tttccattcc cgcaacagat
    12361 ttgccttccg gcgcttatat cctgcatctt agtgatgata agggacgatt tagttatgtg
    12421 cacctgctta agcactgagg gaagatttgc cgcagggctc tttgtatccc gcaactgacc
    12481 tattgagcgg acatttggct gccttcagca ggaagtacaa tatttcccgc ctgcaagcgt
    12541 taaaaccatt tgccctaact ttgtggttcc gttcatgcgg tcgttattca tatatgtaag
    12601 cgttctggca gttattgtta ctgttccaaa ccggtcttcg gcacaggctg ctgaaattgg
    12661 actatggggc ggacttacgc attcttttgg cgatatcaat aattctaccg aaagttttca
    12721 attcctggaa ccgggtgccg gcgtattctt cagatacaat ttgaactcca gaatcggcta
    12781 ttatctcggt ctcagcggcg gccaaacccg tggctacgac agcatttcaa acaatattta
    12841 tcagttgcga cgcaatctga gctaccgcac aaccatttat gacgtgtcgg gtagggtaga
    12901 ctttaacttt ctccccctgg agcgcgaaaa gcctaaatac tggtttacgc cctatgtttt
    12961 catgggactt tctgtgtatt acttcaatcc gcaagcctat tataacgacg aatgggttga
    13021 tcttcagcca cttggaacag aagggcaaca gtttccggag ctcacgggca acgacaaata
    13081 ccagcgtgtg cagatcagtg taccaatggg gggcggattt aaatttgccc tcaataaaaa
    13141 tattaccgcc ggaatcgaat ggaactggca ctggctgttt accgattatc tggacgatgt
    13201 ttctaccgtt tacgtagacc cttccattct ggcaagtggc tcagatggtg cactggcagt
    13261 ggctttggcc gatagaagca cggcaattga catcattccc ctcggcgaac ccgggaaaca
    13321 gcgcggcgat agcgaacatc gcgataaata cctctatacc ggcatttttc tgtcatatac
    13381 tatcgtagac ttaaaatgcg atccacccgg taaatggggt gccaaatttt gataactttg
    13441 cagcccgtta tgtccgcagg tcaaccagat cctaacaaca tcccacgtca tattgtcatt
    13501 atcatggatg gtaatggtcg ctgggcaaaa ctgcgcgata aggcgaggat ctatggtcac
    13561 cacgcaggcg ttgaagcagt gcgaaaagta gtagaagctt gcacagaact gggtgttgag
    13621 tatttgacgc tttatgcttt cagcaccgaa aactggaaac gccctgagga agaggtgaat
    13681 gcactgatgg aactgctcgt aagaaccata cgccgcgaaa cccccgaact cgataaaaat
    13741 aatgtgcgca tcggcatgat tggcgatgga cattctttac ctcctgcttg cattgccgag
    13801 ctcgaagaag caaaaaaaat gactgccgat aacacaggcc tgaatcttat actggctctg
    13861 agttattccg gcagatggga aattacccat gccgttcaac agatcgctaa agagcttgta
    13921 aacggtaaaa taaaacccga agaaattaca gaacaaacca ttgcagataa actcaatact
    13981 gctgccatgc ctgagcccga attgctcata cgcacaagtg gtgaataccg attgagtaac
    14041 tttttgctat ggcaaaccgc ctatacagag tactacttta ccgaaacgct gtggcctgat
    14101 tttgataaag aggcactctt taatgctatt atcgattatc aaaaccgcga acgcaggttc
    14161 ggaaaaacta cggaacaaat tcaagtgaaa tgaaaagaac gctgcttagc ctgttattgt
    14221 gtggagtgat gatcgctaca ttgtccgtta ccggatggtc gcaaacaccg ggactcgact
    14281 ctaatagtgt ggattatggt aatcctaaaa agtataccat cgccgcaacc gaaattcgcg
    14341 gggttacttc acccaatctc gatcttaata gtttaatcgt acgcacaggt cttactcccg
    14401 gattgccact tactattccc ggtgaagatt ttagtgtagc cattaaaagc ctttggaaaa
    14461 tgcgcctgtt ttccgatgtg cagatatttg tagaaaaaac tcttggcgat caggtgtggt
    14521 tgacgattca ggtaacagaa ttgccaaaag tgagtaaata tgcattcacc ggcaccacta
    14581 aatcggaaga tgaagacctt agaccgcaat tggatatcat taaaaatgtg acaccatttg
    14641 ccgattacac caaaacgcat atttacaata ccgttcggga atattatgcg gaaaaaggat
    14701 ttttaaatgc agagatcgat gtttacggaa aaccggatac atcttttaga aatagcgtga
    14761 tcgtgtatat cgatgtagtg aaaaatgcga aagtgaaaat cgcaactatt gatattcatg
    14821 gtaataccga aatgagcgat cgcgaagtgt tgaagaacat gaaggaaaca cgcgtgcgca
    14881 cgcagatcaa gccattctat aacgatcctg aaaatccaat ttccgatgat gtattatcag
    14941 ttaagggtgt agtgaatgtg ctgtcgtcgc tgagcgttag caatatcatg gattttgttt
    15001 acgaccgcgc acaaattcgc attttcaatc agtcgaaata tgatgaagca aaagcgcgta
    15061 ccgatgcaca agctgtaatc acttactata aatcaaaagg ctatcgcgat gctcaaatga
    15121 aactggattc agtgtataat gtaagcgata gatcaattgg attgtcttat accatagatg
    15181 aaggaactaa atattatttc cgcaatatca catggaaggg aaatacgaaa tttaccgata
    15241 aacagctcga ttcaattcta gatatcagca aaggacaagt atataatcag agttatcttg
    15301 atcaacgcat ttccttcgac cttaccaagc cggatatcac gtctaaatat atggatgatg
    15361 gctatttatt tttccgtatc aatcctgtgg aaatttgggc acaggacgat agtattgacc
    15421 ttgaaattcg tatatcagaa ggacctcagg ctatcatcga caaaattatt atcagcggaa
    15481 atacaaaaac caatgaacat gtgattcgtc gtgagttgcg tactttaccc ggacagaaat
    15541 ttaatcgttc attagttatt cgttcacaac gtgagatcat tgcacttgga ttatttgatg
    15601 cggaacaaat tggtatcaac ccgattcctc accctgaatc aggaacagta gatattgaat
    15661 ataccgttgt agaaaaacca tctgatcaat tagaattgtc ggcaggttat ggcggacagg
    15721 gatatggtgt tctcgcatca gttggtattg tattaaacaa ctggagtacc acacaaatgt
    15781 ttaaaaagga aggatggaag ccggtgccaa tgggcgatgg tcagaaattg tctttccgtg
    15841 taaatacaac aggtcgttta taccaagcat ataatatcgg atttgttgaa ccttggttca
    15901 gaggtaaaaa accaaactct ttgtcattca gtttaacacg tacgcagctt ggatataata
    15961 ttcccggttc tacttacgat tcactcgaag ggaaattcat cgcaaatggt gcatctctcg
    16021 gatatggtat tcgcttgaaa tggcctgatg attattttac gttagtaagt actgtgagct
    16081 tctacaatta cagccttact gactatcctt atttcattgc cacgaatggc gattacaata
    16141 acttcagcat taaagaaacg cttggtcgca actcattaga cgatcagcag tttccaaaat
    16201 caggaagcag catttcactc tcgttgcaat ttacaccacc gtattccgct ttaaattcaa
    16261 aagattatgg tgaattggga acggaggaga aatataaatt cattgaatac tacaaatggc
    16321 gtttcaatgc cgattggtat acacctgttg taggcaaatt tgtcttcaga gcatctatga
    16381 aatttggatt cctaggatac tataatagcg atgtgggtat tccgccattc gaaagattcc
    16441 aattgggtgg tgatggatta tcaggaggtt ataccaacac atttgtcggc acagacatta
    16501 tttccatgcg tggttacgaa gtgttcccgc aaaccaacag cagttctgcg cgcactgagg
    16561 ctatcttcaa caagtatacc cttgaattgc gctatcctat cgtaaaatcg caatctgcca
    16621 atatctatgc tctggctttt gccgaaggtg gaaaccttta tgccgatatc gataattata
    16681 atcctttcga gatcaaacgc gcggccggac tgggtgtcag agcttggttg ccgatgtttg
    16741 gactcctcgg tgtagattat ggcataaggt ttgacgatct ggtaccggga gaccttgaac
    16801 aagccagtgg attcttcgat tacatcaccc gtaatggtaa attcacagtt gttctcggat
    16861 ttgaaccgga ataaaaaaaa cgaaagaaca tgaaaaagca acttatcctc gctaccatcg
    16921 taatggcgtt tgccgttacc gctcaggcac agcgctttgc gtatgtgaat actgactaca
    16981 ttcttgataa tatgcctgaa tataaaaagg ctcaggagca aatcgataaa ctgacacagg
    17041 aatggcgcgg cgaggctgac aaaaagcaaa aagagatcga tgaattgtat cgctccttcc
    17101 aaaatgagca atacctgtta acggaagatc agaagaaagc aaaaatttct gagattgaaa
    17161 ccaaagaaaa agcactgaaa gattaccaga aatcggtttt cggctacgaa ggcttgcttt
    17221 atcaaaaacg tcaggaactg gttaagccaa ttcaggataa agtgtacgaa gccattgaaa
    17281 aatatgcccg tgaacgcggc tatgatttca ttttcgataa atccagcagc actaccatac
    17341 tttgggcaaa tcccgataat gataagagcg atgagattat caagaaattg ggatacacac
    17401 caacaaaaac gaattaacac atttataatc caacaatcag tacaaccata ttatgaagaa
    17461 caaattaatg ctcttattcg ccattgcagt tatggcgatt tcacaagccg acgcacaaaa
    17521 aatcggatac ctcaatacca atgaacttct ggtgcaaatg ccggaagtga aaaaggctga
    17581 ctcagtgctc aatcagtatg ccgaagaaat gcagaaaatc tatgctgctt atgtaatgga
    17641 atatcagcag aaattggccg actatcagaa taacgcagct tcatggagcg aagttaaacg
    17701 cgaatcagtg gaaaaagatt taggtaatct tcaaatacgc atcagcgatt ttgagaagga
    17761 atcaaatgat aaactggaag cgaagaaaga agaactctat ggtcctatcc ttgacaatgt
    17821 gaaagcgaaa atcaaattag taggtgatga aaataaattc accaccattc tcgatggctc
    17881 ggcaatgtta tatgtgggca tcgacgcagt ggatattctg ccattagtga aaaagaaact
    17941 aggattgcaa taatcgcatc cataaattat tgaaaacagg gctgtgtctg atgatgcagc
    18001 cctgtttgtt ttataggcat tgatgccgta aatttgaaat caaacaacca gccatgaaaa
    18061 ggatacttac gctcgtattg gtattaacct tcagtgttca tgcctattcc caatcgaaaa
    18121 aagatcaggc gctggaaaaa gcattgaccg ctattgccat gatggactcc gggtatatta
    18181 ccgagagtat cgatttgctg aacgaggcta tcaaactcga ccctgatcga tatgattatc
    18241 cttatgaagt agcctatgct tattacctga tgaaggatta taataagtct atagaagtac
    18301 ttgaaaaatt atctaaacac aaagatgtta ccgatcaatt atatcagctg ttgggcaata
    18361 gctatgatat catcggcgat cctgataaag catttgacgc atatgacaga ggattgaaaa
    18421 aatttcccaa atccggcata ctttttcttg aaaaagggaa tgtctattgg ggacaaaaga
    18481 aatatgccga agcactgcca ttctatgaag acggtattgc tgcagacccg atgttttcat
    18541 ctaactatta tcgcgcagcc agaatttatt gtgctaccga caatccgttt tttggcatta
    18601 tgtatggaga aatattcttg aaccttgagc cgagtacaga aagatcgcag gaaatcagcg
    18661 cgttgttatt cgatacctat aagtctaata ttcatgttac aagtgatact tccgcatcga
    18721 ttgaattttg tagtaataat ctggtattgg ataaagaatc aatgaaacgc atgagtgaag
    18781 gaatactgcc attctgtatg actgcagaaa ctaccatgct catatatgtc ggattttcca
    18841 aggagataac acttgagtct gtttgcaaat tgcgcagttc atttattgat gcatggtatc
    18901 aaatggggaa tgataaaaaa tataatatcg agctgttcaa attccaaaag gatctggctg
    18961 ataagggata ttatgatgct tacaatcact ggttgtttat gaatggcgat ggtgcggcat
    19021 tcgacgagtg gtataagaca catcaagatg aattcaccaa cttcgcgaat tacgctaatc
    19081 aacataggct gcaattaaat gataagacta aattcctggt tgcagatttt cgatgaacag
    19141 gtgattgcta ttgcaatatc aatttccact acctttgctg catgtttcca ataggcgttt
    19201 tcgattccgg ttatggtgga ctgacggtat tgcgctcatt taaaaaggca ttgccggaat
    19261 acgatttcat ctacctcggc gataatgccc gcacacccta tggaaacaga agtttcgaaa
    19321 cggtatatga atatacactc gaatgtgtca accacctgtt taacatgggc tgccgcctcg
    19381 tgattttggc atgtaatacc gcttctgcaa aagcattgcg cacgattcaa caaaacgatt
    19441 taccaaaaat tgattcctcc agaagagtgt tgggcgtcat caggcctact gccgaaataa
    19501 taggcaaaat gacacatacc ggtactgttg gtgttttcgc taccgccgga actgtgcaat
    19561 cagaaagtta tatcattgag atagctaaag cctggcctaa aattactgtt gtgcaagaag
    19621 cctgccccat gtgggtacct ttgattgaaa ataatgagta taataaaccg ggtgccgatt
    19681 attttgtgca acaacatatg ctgcaattat tgcaaaaggc tcctgagctg gataccatct
    19741 tattgggatg cacccattat cctttattaa ttgaaaaaat aaagcaattc gcaccacaag
    19801 gaactactat catcgcacaa ggtgatattg ttgctgaaag tctggttgat tatttaaaac
    19861 gtcatccgga aatggaaagt cagtgcagta aaaatggcac tatgcaattt tataccaccg
    19921 acgatacggc aaattttaat gcacatgctt ctgcattttt cggcagtgaa gtgcagtcga
    19981 cttttttaca cctttgattt tactgcttct gcaatcgcgg cttccagaaa tggaataaat
    20041 gtctgttcat tggaaggaat ataaaatccc ttggtatgtt gtactttttc aagtgcatcc
    20101 tccaaacgga atgtcgactg atctccggca acaaccaatc ctttttcgcc taattctttt
    20161 actaaatatt cttgttccgt ctgccccgga gtaggaacga aaatgcactt tttgttgagc
    20221 agggcaaggt ccatcaatgt actatatccg ccgcgggaaa taattacatc gcaacgcaaa
    20281 atgagatcat ataattcttt gcccggtaaa tgcgaaatca ccctgatgtt atctgttaat
    20341 tgctcatcgc gatgttcgtc ggagatgcct tgaatcaaaa taaattgatg tggcaataat
    20401 ctaccttgtg ta
//
LOCUS       DAOQHY010000044        20262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1187_length_20262_cov_6.438264, whole genome shotgun sequence.
ACCESSION   DAOQHY010000044 DAOQHY010000000
VERSION     DAOQHY010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 20262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20262
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1187_length_20262_cov_6.438264"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..1682
                     /locus_tag="PLL28_05365"
     CDS             129..1682
                     /locus_tag="PLL28_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68784.1"
                     /translation="MIKACLTFWMAIVSLITLGQTTFSKLYWNDYTISGLGIATDGEF
                     VYSTGLGLYNDDELYLPNVFFLKFDVLGNQIDKTYLIAGENAYYRSGYAGGFELLNDS
                     ILFASGHFSEYDTMFPPWNRKHLGQLYAFNTEGDTLFTRKYKGSGFIEFNRLHLFNDK
                     VICGGFTQDTNGVASNAYLTKVNYEGIQIWELMLGDTIHSEATGLLDTISNSTIIGGG
                     AVVNSISPDGIMRNGKIYKLDASGNVYFSKEIGTPYDDSEIEVKVSKNGQSYLVRQYM
                     DTVINEGDYEYVQYIGKTDTNVNFIWRTFLNDKYYKYIYTLRTFEDGSIVAVGTTIVD
                     ETYEPHGYIVKLDSNGAVLWERDYTNNPEGNHYLYDFQKMPDGGYICSGLGADTIDGV
                     IQSMCWLLRLDSMGCLIPGCDEVSIPEIAVNSETYLSIYPNPFGDAATVEVHVPQDFT
                     INGTQALRLDIYDMQGRLIDGYSNIYPSNPGETIRFKMYRNTMTAGNYLATLKYGDEV
                     LNTIQFVLK"
     gene            1693..1836
                     /locus_tag="PLL28_05370"
                     /pseudo
     CDS             1693..1836
                     /locus_tag="PLL28_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
     gene            complement(1894..2439)
                     /locus_tag="PLL28_05375"
     CDS             complement(1894..2439)
                     /locus_tag="PLL28_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68785.1"
                     /translation="MDSSGKLKMRGLGIEKALTITLEANPARFKELLKRNISEGWNKW
                     SLIPGRNKAPDYLCIMEGFMDNDQFEVKPFGGGMRKRNGRGAIRFKGKISESGNTVTL
                     HITESISPNYLAFIGPIIMVIFLYSQGSSLSIMGLMTVYAILVLLSSIFYYISGIMNI
                     ADFRHQFNKEVSYLARLSLKE"
     gene            2533..3567
                     /locus_tag="PLL28_05380"
     CDS             2533..3567
                     /locus_tag="PLL28_05380"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68786.1"
                     /translation="MKHILGILPILSVLSCTAQQPNATATDLGVMFYNQENLYDTIDD
                     PKVDDAIFLPDAERQWNTEKYNKKLSQMARVMAEGPGSLPALIGMCEVENASVLNDLI
                     KEPVLANAKYKYVHFDSPDERGIDVALLYNPEYFKPTTSTAIPVKLVHGDSTDHTRDI
                     LLVSGTIMQNGKQETLHVFVNHWPSRREGEEASAWKRATAAAVLKAAVDSLNRNYTDP
                     NIIIMGDFNDTPFDASIQKVLGAQQEAATYNDESLVDLMAAKQAGGLGSYNYKGNWQA
                     LDQIIVSETMLDGTGLQIDGDKTTFIRKDWMLFHHNTYGDSPNRTYSGTKWYGGFSDH
                     LPAYTALLIK"
     gene            complement(3574..4398)
                     /locus_tag="PLL28_05385"
     CDS             complement(3574..4398)
                     /locus_tag="PLL28_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68787.1"
                     /translation="MIEMQYPSKLMLFGEYAVIAGGEGLAIPWPDFFAEWVEQPETAS
                     AYRPHLERFAKWLYEQGFDSILDTDTFLQELEDGLDMHSNIPIGYGVGSSGAVVAAVY
                     DRYAKEKLPISDLIALRNTLASMENFFHATSSGLDPLVCYVQQPIHITPYAINVVSTP
                     ISFGNLQMRLLDCGFARATHPLVSAFKEKLSDPHFHTALQEYIQLSNACIEHVLSWHE
                     TKFEQALRKLSAWQFHNFDFAIPEHLRKEWQDAVLSGNCFYKLCGAGGGGFMLVFS"
     gene            complement(4398..5432)
                     /gene="mvaD"
                     /locus_tag="PLL28_05390"
     CDS             complement(4398..5432)
                     /gene="mvaD"
                     /locus_tag="PLL28_05390"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HNF68788.1"
                     /translation="MQINWKSPSNIALIKYWGKHGVQLPNNPSLSLTLREANTTTSLD
                     LKKKRSKKNIEIDFLFEGERNDKFAAKLETFLASVTQELPWLTKNRLVLRSENSFPHS
                     AGIASSASSMSALALCLMSTNRMLTKTPEDDATFLRMASRLARLGSGSAARSVYPGFT
                     VWGKTTSVKNSSDDHAIIAPANYHADFNELQDSILIVHAGEKSVSSRAGHALMNDHPM
                     AAQRYKNAHARLKQLLDALKHADWQLFCKVVETEALELHALMMTSDPSFILMHPNTLA
                     IIERIRFFRATTNIPVCFTLDAGPNVHILYPYFAKKEVRNFIQSELLQFCVKKKVIYD
                     QMGDGPSMLL"
     gene            complement(5460..6404)
                     /locus_tag="PLL28_05395"
     CDS             complement(5460..6404)
                     /locus_tag="PLL28_05395"
                     /inference="COORDINATES: protein motif:HMM:NF040656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYDIA family GHMP kinase"
                     /protein_id="HNF68789.1"
                     /translation="MQRDFYAHGKLLISGEYLVLKGALALAVPTVYGQHLSATIEQKN
                     ADVINWTSFDNNGNKWFNATLDAKSLQVKGPLTGDKNIADTLRNIIIAIRKQQPQYLL
                     QGDINISTKLEFPNDWGLGSSATLLTNLCKHANADAMIVNAEVFKGSGYDIATAQANA
                     PILFRIDDEGPVFDSVNFFPPQHEQFRFVHLGEKQNSRIAIEQFLDETKDKKLFNEIE
                     TISEISEALLFCDDFSDFMQLLDDHEEMMQYVLQEEKIKTARFNDFPGVVKSLGAWGG
                     DFVLAAADMDGADIDTYFKEKGLHTVISYADMVLSAHA"
     gene            complement(6454..7794)
                     /locus_tag="PLL28_05400"
     CDS             complement(6454..7794)
                     /locus_tag="PLL28_05400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HNF68790.1"
                     /translation="MQKIKGFSKLSKTDKIAWLAKHFLTSNPLDIAREFSSYMHSNAE
                     MQKLFDGISENTLTNFYLPYGVAPNFVINGRTYVVPMVIEESSVVAAAAAGAKFWSDR
                     GGFNAEVISTTKVGHVHFLWRGDSEKLFHFFDTELKEALLAEVKHLTANMESRGGGLR
                     TLELIDCTELEPEYFQLKATFETMDAMGANFINSVLEAFAECLTEEVKHYKDFSGAEK
                     NAEVVMSILSNYTPECLVKAWVECKVSDLGGFPEANLTAQQFARKFSTAIRIAKHDVY
                     RATTHNKGIFNGIDAVVLATGNDFRAIEACGHAYASKNGQYGSLSDCEIVGDTFRFSI
                     TLPLAVGTVGGLTKLHPLAKRSLEMLGNPSAKELMMIIATVGLAQNFSAVKALVTSGI
                     QKGHMKMHLTNILRHFGATDTEIQLATTYFADKTVSFSSVRDFLNNSRNLATEK"
     gene            complement(7843..8871)
                     /locus_tag="PLL28_05405"
     CDS             complement(7843..8871)
                     /locus_tag="PLL28_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68791.1"
                     /translation="MLTFTTVNDPNSASRKQDHIELAFKSQAAAAENDRRFYYEPVMG
                     FHRAEVSLKTSFLGKTLGMPLWISSMTGGTEQAGTINKRLAEACAEFGLGMGLGSTRR
                     LLDDNTWFEDFNLRPVIGPELPFFANLGIAQVESLLAERKTDKIHALIARLQADGLII
                     HINPLQEWFQPEGDRFKRSPLETIQEFIESADYQVIVKEVGQGMGPNSIKALLQLPID
                     ALDFGAFGGTNFSKLELLRNRSGDASLYEDLAYIGHTAAEMTGFVSEAVWELGEKVQC
                     KQVIVSGGIGNFLDGYWCMEKLPLTGIYAQGSAFLKFAQLDSRALKNYIVSQREGLIL
                     CKQFLRVR"
     gene            complement(9008..9463)
                     /locus_tag="PLL28_05410"
     CDS             complement(9008..9463)
                     /locus_tag="PLL28_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68792.1"
                     /translation="MPIAMTLFVLVLLGFIIGSFIDGSITPSIGIFAFMLFFIFLLIW
                     IVFGELRTKVIRVELKDDAVMVSSYLGLGKKRMYNLTQFEGFETAILPSNYDKYEYLY
                     LMTNGKKVVKLSQFYHKNYNELKTVIEGTVTNLGEKSFSMIEEFKEIFQ"
     gene            9837..11534
                     /locus_tag="PLL28_05415"
     CDS             9837..11534
                     /locus_tag="PLL28_05415"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNF68793.1"
                     /translation="MKFSSFPYHRPDMSALQAQFEVALGKFEHAASAENQLTAMQEIN
                     AVRSAFDTMYQICSIRHSIDTRDTFYTEEQAYFDEHGPEMEALISKYYQVLVKARFRE
                     AIEQKYGKQLFRIAEMQLRAFHPDIIPDLINENKLNTAYQELVASALIPFDGAERTLA
                     EMGPYMKSVDRNVRERAAAARWQFFEDNRAKFDTIFDDLVKTRHRIAKTLGFDNFIAV
                     GYLRMQRSDYNAEMVDVFREQVRKYIVPLATNLRNQQALRLGISTLKYYDEGLQFTSG
                     NATPKGSPEFIIGKGGEMYREMSPETAKFFHEMLDNELMDLVAKKGKAGGGYCTYISG
                     HRAPFIFSNFNGTSADIDVLTHEVGHAFQVFMSRGFDTPEYFWPTSDAAEIHSMSMEY
                     FAYPWMDQFFNGEADKYRYAHLVESLQFLPYGVAVDEFQHRMYAEPELTPLQRKNIWR
                     EIEKKYLPHRNYDGNIFLEEGGFWQTQAHIYQSPFYYIDYTLAQMCAFQFWMMAQEDT
                     TKAFNNYVELCKAGGSKSFLELVAYAKLKSPFDPQAFADTIGHIDNYLKGVDDLQLN"
     gene            complement(11620..13110)
                     /locus_tag="PLL28_05420"
     CDS             complement(11620..13110)
                     /locus_tag="PLL28_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68794.1"
                     /translation="MKKQDTSLFDLIKSMSMAEKRYFKLFATRHFPKDSTYHRLYEAI
                     DAQDKYREQTLTKQFATGKSASYFPVLKKQLYDALLEALHRFEAYDDEVHKLTLGVHY
                     CSLLLNRGLYAACAKQIKKYKAKGYAFEKFEEVIALIAIEKRLAARTQYTDYNHNGLA
                     ALHREELHCLEQIKITSEYWINSAAIYKMHIEKKLGPGRDNHALKSLAASPLFADASS
                     ATTFRARLDRMQVLALYAFAEQQTETAFDYNSAFLQLMDDQPYLRQQYADRYFATLNN
                     YLIDCLILQKHEALIAGIQTLRKLPEEDAFRHMQHLDAQVFRMSYLLELNYYVGLEDF
                     VHALSVATAVLAQITNVAPFIARPNLITLYYLCAYVLFCNKQFDQCLDALQTLMDMKD
                     AETVIDLFTDSRMMQILCHFELGNILLIDSLIVSFNRHLESRKLKASTYATVLRALKQ
                     HMRSPLKPLKQKLHQQLQALSQQPEEKRLYNNFNYFWWLERVSSLQ"
     gene            13257..15371
                     /locus_tag="PLL28_05425"
     CDS             13257..15371
                     /locus_tag="PLL28_05425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68795.1"
                     /translation="MKKMYTIIAAILLSTAVLPLGAQSWVNYSNLSYVTDAEPSGTDI
                     WITAKGGVQLFNTLTGTSMFFRKGDAGLPSSSVEQVAKSNATGVIWVGTYDAGVVEWD
                     GDSWLTYPFPSAMLLYRMKFDGFGNLWLQTDAGLFHFNTTDHVYTFVNSTGGAGWDFN
                     AWDFDITPDNKVIIFTGTNCQVIDAATLAAIDSFPNSDSPNVLACSPSNVRVYNVDEE
                     TYLINNGFAIEFEFKDNTFTPATDGLPEFAFVQNIIRGTDGALYAYVNNASVYRLDGL
                     TWTYVDNAAVYFPEFLVYTDGVEFYFSSNNYASTPGVIHMSETDYVKISSLEYAFTSN
                     AIKGLTEDADGNIFILSGSGQYQYNELTDDFSLFGNVPSIYGTIYDLRSVNGIFYCVN
                     YGDLLEYNNGTSWVNIPYADGYTSPYIFDYDVTSDGTIYFVNDDGAFKYKDGETTCLF
                     PTPSLYDWGLSVTYDATRNLLWIGRMDGILKYDFVTQELIDAMDVPAMSDGMAIQEIK
                     VAPNGDVWFGANNNKAYMYDGTEWTDYNVGTGMGFVTSFDFEGANIYFGITDGNGGVH
                     VWDTSNDNWSWLSSATDPTLVSDEVSYMTVTTGGDLWIAHVNAGVSRLDGTPSVGIDE
                     VTANNAIIYPNPVAQTLFVSGLEDGNYQYVISDLSGKQLWSSSQATHSINVSQFPSGI
                     YLLQVRAGDKSVFNGTFIVPTK"
     gene            complement(15474..16184)
                     /locus_tag="PLL28_05430"
     CDS             complement(15474..16184)
                     /locus_tag="PLL28_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF68796.1"
                     /translation="MEEIKTKILLVEDDPNFGAVLKDYLTMNGYEVRLATDGEKGEDL
                     FFKEEFNLCVLDVMMPKKDGFSLAAQIKRVNPDQPVIFLTAKTLQDDVLEGFKTGADD
                     YITKPFNSEELLYRIAAVLKRGGKARTNNQQKEFNVGKYHFNYKTRMLKNGDSEAKLS
                     PKEAELLRLFCMKVNDIVYREEALKQIWGRDDYFTARSMDVFVTKLRKYLKDDPTLEI
                     VNIHGNGFRLNAEETANI"
     gene            complement(16203..17777)
                     /locus_tag="PLL28_05435"
     CDS             complement(16203..17777)
                     /locus_tag="PLL28_05435"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNF68797.1"
                     /translation="MSTALLGIILVQVYWIQNTIEQKEQIFNYHVNEALNRVADKVET
                     NIAASVLSSQMNLFFSDSTYWMGTGDSLTTYENVLSDSTMAGMLESLRSNQAYYNDPE
                     YTTIEPFADALQGMPGSQKPTMILEPLELIGDNSSPNDALVSQILSDIDRQLQLNSGR
                     IKKAMEQMMIRMVQRGITPEQTIDTNFLKNSLYHELNNRGITTDFNFGVLMHDKFFIS
                     NAEDNFGLEKLAASSHKVSLFPDDIVFNNDVLLVSFPHQKSFILSSIWSLLLGSLLFT
                     TIIVAVFTYTIFILVKQKKMSEIKNDFINNMTHEFKTPLATISLAVDAVNNPMILQDQ
                     QKVKYYTGIIRDENKRMNAQVEKVLQMALLDKHQVSISNDDVDIHEIIYRAVENISLL
                     VEEKGGTVETELLADRFEITGDEVHLSNVISNLLDNANKYTPEHPHIVVRTRSDNKGI
                     YITVEDNGIGMSNDDMSMIFEKFYRVPTGNVHNIKGFGLGLTYVKTVVEAHKGSIEVK
                     SQLKKGSQFRIFLPHQ"
     gene            17980..19437
                     /locus_tag="PLL28_05440"
     CDS             17980..19437
                     /locus_tag="PLL28_05440"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNF68798.1"
                     /translation="MQRFLFSITTLIIPAMLFAQQPDNQIHLRIDANVDGQQVKIDTF
                     IESLGDLNLDEFLQGLGLENELNQLNIDINSGFGVMPSFDEEAMQEMLKSLSELEMPE
                     MPELPELPGIDMGEAMSFGYAGENRAFLGVYTNKVTEGAQISDIVEGSAAESAGLMEG
                     DIITHIDARSIESPANLMEVLALYYPGDEVTVTYIRAGKTDKVKLTLQENTNASSTWG
                     SQNYNFNFDFPDSIFMGQGNYLIETPAHGYLGVYLEDADGKVLVTGIEGGSAAEKAGL
                     KADDILLEIKGNKVQSYDEVLEIMQETKPDEKIHIIYERGGKRKETDATLKSAGGGAY
                     YFNFNNDEGYNNDIRIAPCLPTPPGSSYSYYSRDSSKTINICITGAYAGDYGDSTGGF
                     LPDQAHPLMDPNNLAVYANPSDGTFRIHVDLPEEGDAKVVITDMAGKEVYNELLPNFS
                     GTFDRTVTLSNASKGTYFVKVTQKGYSSTRTVVIQ"
     gene            complement(19513..19878)
                     /locus_tag="PLL28_05445"
     CDS             complement(19513..19878)
                     /locus_tag="PLL28_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNF68799.1"
                     /translation="MKMKKATSVEGYIADFPKDIQLKLMEIRALIKKTAPKAEEGISY
                     GMPGYKLNGPLVYFGGFKQHIGFFPTGEGVAEFAEELKDYKTSKGTIQFPYDKPLPKT
                     LITRIVKHRIKVNEAKTKK"
     gene            20009..>20262
                     /locus_tag="PLL28_05450"
     CDS             20009..>20262
                     /locus_tag="PLL28_05450"
                     /inference="COORDINATES: protein motif:HMM:NF024953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNF68800.1"
                     /translation="MKKKNIIYWVVTGIVAAYMVFSALPDALLQDKAVEFMQGLGYPD
                     YFTRFIGIAKLLGVAGILLPLPLRLKDWAYAGLVFDLVGA"
BASE COUNT         5760 a         4005 c         4406 g         6091 t
ORIGIN      
        1 actcgataat ttggtttcct acattaagtt agataattct atgctgaagc ctggtgtgta
       61 tattggttac gtatctggta atttaggaga gcaaataagt aagcaaatta ttgttataca
      121 gtaatcgtat gataaaagct tgtctgacat tttggatggc aattgttagt ttgattactt
      181 tagggcaaac gacattctcc aaattgtatt ggaacgacta tactattagt ggattaggga
      241 ttgcaacaga tggcgagttt gtgtattcaa cagggttggg actttacaat gacgatgaat
      301 tgtatcttcc aaatgttttt ttcttaaagt ttgatgtctt aggtaatcaa atcgataaaa
      361 cctatttaat tgcaggagag aacgcctatt atagaagtgg atatgcgggt ggattcgaat
      421 tgttaaatga tagtatctta tttgcttcag gccactttag tgagtatgat actatgtttc
      481 caccgtggaa caggaaacac ttgggacaac tatatgcatt taatacagaa ggtgatacgt
      541 tgtttactag aaaatataaa ggaagcggat tcattgaatt taatcggctg cacctgttta
      601 atgataaagt tatttgcggc ggattcacgc aggatacaaa tggtgttgca agcaatgcgt
      661 atttgactaa agtaaattac gaaggtattc aaatttggga actgatgcta ggtgatacta
      721 ttcattctga agctacaggt ctactagata ccatatcaaa cagcacaata atcggtggag
      781 gcgctgtcgt aaattctatc agcccagatg gaattatgcg caatggtaaa atctacaaat
      841 tggatgcatc gggaaatgtc tatttctcca aggaaatcgg taccccatat gatgattctg
      901 agatagaggt aaaagtgtcg aagaatggtc agtcgtattt agtgcgacaa tatatggaca
      961 cagtgattaa cgaaggagat tatgaatatg tgcagtatat aggcaaaacg gatacaaatg
     1021 taaacttcat ttggcgtact tttttaaatg ataaatatta caaatatatt tatacccttc
     1081 gcacatttga agatggaagc attgttgctg ttggaacaac tatagtagat gaaacttatg
     1141 agccacatgg ctatatagtt aaattagaca gtaatggcgc agttttatgg gagcgggatt
     1201 atacaaataa ccccgagggc aaccattatc tatatgattt tcaaaaaatg cctgatggag
     1261 gatatatatg ctctggtttg ggagcagata ctatagacgg tgtaatacaa tcgatgtgtt
     1321 ggcttttaag attggacagc atgggttgcc tcattccggg ttgtgatgag gtgtcgatac
     1381 ctgaaatagc tgttaattct gagacttatc ttagtatata tcctaacccg tttggagatg
     1441 cagcaacagt agaagtccat gtgcctcaag attttaccat aaacggcacc caggcactca
     1501 gacttgatat ttatgatatg caaggcagat tgatagatgg atacagtaat atttatccaa
     1561 gcaaccccgg agaaaccatc cgttttaaaa tgtatcgcaa tacaatgacg gctggaaatt
     1621 acctcgcaac gcttaagtat ggggatgaag ttttaaacac aattcaattt gtgctaaagt
     1681 gattgatttt ttttgtatct cgccctcagc tacgatcacc gcatcatcga cggcaaagaa
     1741 agtgtaagct tcttagttcg cgtaaaagag caacttgaaa agcctgagca actattgttt
     1801 ggaattgatc cggtggggtt gttgttggag atgtgaattc aaatacgatt tctgtttgta
     1861 aacgaatgac tatgcatttg ttgtaaaagc agattactcc ttcaaactca accttgccaa
     1921 ataagaaacc tccttattaa attgatgtct gaaatctgca atattcataa tacctgatat
     1981 gtaataaaag atagaggata ataaaacaag aattgcataa acagtcatta agcccataat
     2041 ggagagtgac gacccctgtg aatataaaaa tatgaccata atgatggggc ctatgaatgc
     2101 taagtaattt ggactaattg attcagtgat gtgcagggta actgtattac ctgattcgct
     2161 tattttccct ttaaaccgaa tcgcacctct gccatttctt tttcgcattc cgccaccgaa
     2221 aggtttaact tcaaattggt cgttgtccat gaagccttcc atgatgcata aataatccgg
     2281 tgccttgttc ctgccgggaa tcagcgacca tttgttccat ccttcgctga tatttcgttt
     2341 tagcagttct ttaaaacgtg caggattggc ctccagtgtt atggtgaggg ctttttctat
     2401 tcccaaacca cgcattttca acttgcctga ggaatccata agggtaaatt taactaaaag
     2461 cacattgcct tcaaattggc atagggtttg gatttatccg ctacctttgt cacctgtaaa
     2521 agacgaccgg caatgaaaca tattttaggg atactaccga ttttgtctgt gctctcctgt
     2581 acagcgcagc aaccgaatgc tactgctacc gaccttggtg tgatgtttta taatcaggag
     2641 aatctgtatg ataccatcga tgatccgaaa gtggatgacg ctattttttt acctgacgct
     2701 gaacgtcagt ggaataccga gaaatacaat aagaagcttt cgcagatggc tcgtgtaatg
     2761 gctgaaggcc ccggctcatt gccggcattg ataggtatgt gtgaggtgga aaatgcttct
     2821 gtattaaacg acttgattaa ggagcctgtg ttggcaaatg caaaatataa atatgtccat
     2881 ttcgattcgc ctgatgaacg cggaattgac gtggcattac tttataatcc ggaatatttc
     2941 aagcctacta catcaacagc aataccggta aaattggtac atggtgattc cacagatcat
     3001 acacgcgata tattacttgt ttcgggaaca attatgcaaa atgggaaaca ggaaacattg
     3061 catgtatttg taaatcattg gcctagtcgc cgcgaaggtg aagaagcgag tgcatggaag
     3121 cgcgctacag ccgcagcagt gctcaaagca gctgttgata gtctcaatcg caattatacc
     3181 gatccgaata ttattatcat gggcgatttt aatgacacgc catttgatgc ttccatacaa
     3241 aaagtgttag gtgctcaaca ggaggctgca acctataatg atgaatcgct tgtagacctg
     3301 atggctgcaa aacaggcagg tggattgggg agctataact acaaaggaaa ctggcaggca
     3361 ttagaccaga taatagtttc cgaaaccatg ctcgatggaa caggtttgca aattgatggc
     3421 gacaaaacaa catttatcag aaaagactgg atgttgtttc accataatac ctacggcgat
     3481 tctccgaaca gaacatacag cggaacaaaa tggtatggtg gtttttcaga ccacctgccg
     3541 gcgtatactg cactacttat aaagtgatgc gtgttatgaa aataccagca taaatccacc
     3601 tccgcctgct ccgcataatt tgtagaagca gtttccggat aatactgcat cctgccattc
     3661 cttgcgaagg tgctccggta ttgcaaaatc aaaattatga aattgccatg ccgataattt
     3721 gcgcaatgct tgttcaaatt ttgtttcatg ccaactcaat acatgctcaa tacatgcatt
     3781 tgataattgg atatattcct gcagtgcggt atgaaaatga ggatcgctca gtttctcttt
     3841 aaatgcagat accaacggat gtgtagcacg tgcaaatccg cagtcgagca atcgcatttg
     3901 taaattgcca aaagaaattg gtgtgcttac cacattaata gcataaggcg taatgtgaat
     3961 gggttgttgc acataacata ccaaaggatc aagaccgctg cttgttgcgt gaaaaaagtt
     4021 ctccattgat gcaagtgtat tgcgcagtgc aatcaaatca gatatcggta atttttcttt
     4081 tgcgtatcgg tcataaacag cagcaacaac tgcacctgaa ctgccaactc catagccgat
     4141 aggaatattg gaatgcatgt cgaggccgtc ttccaattct tgtaaaaaag tgtcggtatc
     4201 taatatgcta tcaaagcctt gttcataaag ccatttggca aatcgttcga gatgcggacg
     4261 atatgctgaa gctgtttcag gttgttctac ccattcggca aaaaaatcgg gccagggaat
     4321 agccaatcct tctccgcctg ctattacggc gtattcaccg aataacatca gcttggaagg
     4381 atattgcatt tctatcatca caataacatt gaagggccat cgcccatttg atcatagatc
     4441 acttttttct tcacacagaa ttgcagtaat tctgattgaa taaaattacg cacttctttt
     4501 ttagcgaaat atggatacag gatgtgtacg ttaggtcctg catcaagggt aaagcaaaca
     4561 ggtatattgg ttgttgccct gaaaaatctg atacgttcaa taatggccaa cgtatttgga
     4621 tgcatcaaaa taaatgatgg atcactcgtc atcatgagcg catgtaattc taaggcttcc
     4681 gtttcaacta ctttgcaaaa taattgccag tcggcatgct tcaatgcatc caataattgc
     4741 tttaatctag catgggcatt tttataacgt tgtgccgcca tcggatgatc gttcatgagt
     4801 gcatggccgg cgcgactgct caccgatttt tctccggcgt gcacaatgag tatactatct
     4861 tgtaattcat taaaatcggc atgatagttt gcaggagcga taatggcgtg atcgtcgctg
     4921 ctgtttttta ccgaagtggt ttttccccaa accgtaaatc cgggatacac actgcgcgct
     4981 gcagaaccac tacccaatct ggcgaggcga ctagccatgc gcaaaaaagt agcgtcgtct
     5041 tcaggtgttt ttgtgagcat gcgatttgtc gacatcaggc ataatgccaa tgcgctcata
     5101 gatgatgctg agcttgcgat acctgccgaa tgcggaaagc tattttcact gcgtaatacc
     5161 aatctgtttt ttgttagcca tggtaattcc tgtgttacag aggcaagaaa tgtttccagc
     5221 ttggctgcaa atttatcatt gcgttcacct tcgaataaga aatcaatttc aatatttttt
     5281 ttcgaccgct tttttttcaa atcaagcgac gtggtggtat ttgcctctcg aagagtgagt
     5341 gataagcttg gattattggg caactgtaca ccatgcttcc cccaatattt gatgagggca
     5401 atgtttgacg ggcttttcca gttaatctgc attgtgtaga attgtttagg aatcattatt
     5461 tatgcatgcg cagacagcac catatccgca taggaaataa cggtgtgcaa tcccttttcc
     5521 ttgaaatagg tatcgatgtc cgcaccatcc atatcggcgg cagccagcac aaaatcgcca
     5581 ccccaggcgc caagagattt tactacaccg gggaaatcat tgaatcgtgc tgttttaatt
     5641 ttttcttcct gcaacacata ttgcatcatc tcttcatgat catcgaggag ctgcatgaaa
     5701 tctgaaaaat catcacaaaa taaaagtgct tcactgattt ctgagatggt ttctatttca
     5761 ttgaacaatt ttttatcttt tgtttcatcc agaaattgtt caattgcaat acgtgaattt
     5821 tgtttttctc cgaggtgcac aaatcggaat tgctcatgtt gtggtgggaa gaaatttacc
     5881 gaatcgaaaa ccggaccttc atcatctatc cgaaataaaa tcggtgcatt agcttgtgcc
     5941 gtggcgatgt cataaccact gcctttgaac acttctgcgt tcacaatcat ggcatctgcg
     6001 ttggcatgct tgcatagatt agtgagcaaa gtggcgctgc tgcccaatcc ccaatcattt
     6061 ggaaattcga gtttcgtgct gatattaata tcgccctgca ataaatattg tggctgttgt
     6121 ttgcgaatgg caatgatgat gttgcgtaaa gtgtcggcaa tatttttgtc gccggtcaat
     6181 gggcctttta cttgtaatga tttagcatcc agtgttgcat tgaaccattt attaccattg
     6241 ttatcgaagc ttgtccaatt gataacatcg gcatttttct gttcgatggt tgcagataga
     6301 tgctgaccat aaacagtagg tacggcaagt gcgagagcgc cttttaatac taagtattcg
     6361 ccgctgatca ataatttacc atgagcgtaa aaatctcttt gcatgacggt agtaaagata
     6421 cgtgaaggaa tgcgctttac ttactgtgcg acattatttt tctgtagcca ggttgcgact
     6481 gttattcaga aaatcgcgca cactgctgaa ggatactgtt ttatcggcga aataggttgt
     6541 agccagctga atttcggtat ccgttgcgcc gaaatgacga agaatattcg tgaggtgcat
     6601 tttcatgtgc cctttctgaa tgccggaagt aacgagtgct tttacagcgg agaaattttg
     6661 tgcaagacca accgttgcga tgatcatcat taattccttg gcactaggat tgcccagcat
     6721 ttcgagtgag cgtttagcga gcggatgtaa ttttgtgagt ccgccaacag tgcccactgc
     6781 aagcggtagg gtgatggaaa aacggaaagt atcaccgacg atctcgcaat cagagagact
     6841 gccgtactga ccatttttag aggcataagc atgaccgcaa gcttcgatgg cacggaaatc
     6901 gtttccggta gcaagaacta ctgcatcaat gccattaaaa attcctttgt tatgtgttgt
     6961 agcgcgatat acatcgtgtt tggcgatgcg gatggcggta ctgaatttac gtgcaaattg
     7021 ctgtgctgtg aggttagctt ccgggaatcc gccgagatcg cttactttac attcaaccca
     7081 ggcttttacg agacattccg gagtgtagtt actgagaatg ctcatcacta cttctgcatt
     7141 tttttctgcg ccggaaaaat ctttataatg ctttacttct tcggtcagac attcggcaaa
     7201 tgcttcgaga acagaattga taaaattcgc acccatggca tccatggttt cgaaagttgc
     7261 tttcagctga aaatattcag gttccagctc cgtgcaatct attaattcga gcgtacgcaa
     7321 accaccaccg cgcgattcca tatttgcagt gaggtgtttc acttctgcca ataaagcttc
     7381 tttgagttct gtatcgaaaa agtggaatag tttttcacta tcgccgcgcc ataaaaaatg
     7441 cacgtggcct actttagtgg tggaaatgac ttcagcatta aatccaccgc gatcgctcca
     7501 gaattttgca ccggcggcgg ctgcggccac cacagaagat tcttcaatca ccatcggcac
     7561 cacatacgtg cgaccattga tcacgaaatt cggggcaacg ccataaggga gatagaaatt
     7621 ggtaagcgta ttttcagaaa taccgtcgaa cagtttctgc atctcggcat ttgagtgcat
     7681 atagctcgaa aattcgcgcg caatatccaa aggattgctg gtcagaaagt gtttcgctaa
     7741 ccacgcgatc ttatctgttt tactcagttt cgaaaatcct ttgatctttt gcattattcc
     7801 aaatttagaa cccctgcaac tctgcggctc tgcgtgaaaa aattatcgca cccgaaggaa
     7861 ttgcttacac aaaattaacc cttcccgctg actaacgata tagtttttca acgcccggct
     7921 gtccagctgt gcgaatttca ggaaagccga tccttgggca tagatgccgg tgaggggtaa
     7981 tttctccata caccagtatc cgtccaggaa attgccgata cctccactca cgatgacctg
     8041 cttacactgc accttttcgc ccagttccca tacagcctca cttacaaatc ccgtcatttc
     8101 agcggccgta tgacctatat acgccagatc ttcgtaaagg gatgcatccc ctgatctgtt
     8161 tcttaacaat tcaagcttag aaaagtttgt acccccaaaa gccccaaagt ccagtgcatc
     8221 gatagggagc tgcaggagtg ccttgatact attgggcccc attccctgtc ccacttcctt
     8281 cacaatcacc tgataatcag cactttcaat aaattcttgt atcgtttcaa gcggcgagcg
     8341 cttaaatctg tcgccctccg gttggaacca ctcctggagg ggattgatat gtataatcaa
     8401 gccatcagcc tgtaaacggg caattagggc atgtatctta tctgtttttc gctccgccag
     8461 aaggctttcc acttgtgcta tgcccagatt tgcgaaaaag ggcagttcag gacctatcac
     8521 cggcctcagg ttaaaatcct caaaccaagt gttgtcgtcg agaaggcgtc ttgtggagcc
     8581 gagtcccata ccgagtccga attccgcaca agcctcagcc aatcgcttgt tgatggttcc
     8641 tgcctgttca gtgcccccgg tcatactgct gatccacagt ggcattccca acgttttacc
     8701 caagaagctg gtcttcagcg atacctctgc ccgatgaaac cccataaccg gctcataata
     8761 aaacctgcgg tcgttctcag cggctgcagc ctgactttta aaagctagtt cgatatggtc
     8821 ttgcttgcgc gatgcagaat ttgggtcgtt cacggttgta aaggtaagca aagtaggaat
     8881 tagccgatta gccgattgag taatgtgaga aaaattgtgc aataaactct gcggctctgc
     8941 gcctctgcgt gaaattttgg ggattctaat gaaatgaata aacgtgattc acttatttga
     9001 attagtatta ttgaaaaatc tctttgaatt cttcaatcat actgaaactt ttttctccca
     9061 ggttggtaac cgtaccctcg ataacagttt tcagttcgtt gtaattttta tgatagaatt
     9121 gagaaagctt aaccactttc ttcccgttgg tcattagata taagtattca tatttatcat
     9181 aattcgaagg taaaatagca gtttcaaacc cttcgaattg tgtgaggttg tacattcttt
     9241 ttttgcctag tcccaaataa cttgaaacca ttacggcgtc atccttcaat tcaacgcgaa
     9301 ttactttcgt ccgcagttct ccaaaaacaa tccatatcaa aagaaaaata aaaaacagca
     9361 tgaaagcaaa aattccgatc gaaggggtaa ttgaaccatc tatgaaactt cctataataa
     9421 atcccaatag taccaataca aaaagcgtca ttgcgattgg cattgctatc cggaatttga
     9481 atttggagtg gaggtgcatt aagattggtt aatgagttta caagtttgcg tgcagcaggc
     9541 tttttccatt gatgtttatt cgaagataca aagtttcaaa aaccatcaga aaactgatct
     9601 ccaacacaga tagtcttcct gccgcgagat atgtgggacg gaggggaagt tttaagcgca
     9661 gaggtccaga gtgcggagtt gtaaacttca gcattccgtg agcgcttacc ccaattggct
     9721 aatcggcaca ttggctaatc ggctaattgg ctaattagct aattgatcgc ctcctgttat
     9781 agtaaagatc tcatgccata gcgtccatgc aatcccatgt accttatctt tgccccatga
     9841 aattctcctc ttttccatac catcgtcccg atatgtctgc gttgcaagcg cagtttgaag
     9901 tggcgttggg aaaatttgaa catgcggcaa gtgcggaaaa tcagttgact gctatgcagg
     9961 aaatcaatgc cgtgcgcagt gcatttgata caatgtatca gatctgttct attcgtcact
    10021 ctattgatac cagagataca ttttatacag aagaacaggc atatttcgat gagcatggac
    10081 cggagatgga agcgctcatc agtaaatatt atcaggtatt agtgaaagct cgttttcgtg
    10141 aagcaattga acagaaatat ggcaagcaat tatttcgtat cgcggaaatg cagttgcgcg
    10201 catttcatcc ggatattatc ccggatctga tcaacgaaaa taaattgaat acggcatatc
    10261 aggaattggt tgcatctgct ttgattccat ttgatggtgc tgaacgaacc ctggctgaaa
    10321 tggggccata catgaagagt gttgatcgca acgtacgtga acgtgctgcg gctgcacgct
    10381 ggcaattttt tgaggataac agagcgaaat ttgatactat tttcgacgac ctggtgaaaa
    10441 cgcgtcatcg cattgcaaaa acactgggct ttgataattt tattgctgtc ggctatctgc
    10501 gtatgcaacg cagtgattat aatgcagaga tggtagatgt atttcgcgag caagtgcgta
    10561 aatatattgt gccgctggct accaaccttc gcaatcagca agctttacgt cttggaatat
    10621 cgacattgaa atattacgac gaaggattgc agtttacatc aggcaatgca acaccaaaag
    10681 gatcaccgga atttattatt ggaaagggtg gcgaaatgta tcgtgaaatg tcgccggaaa
    10741 cggcaaagtt ctttcatgaa atgcttgaca atgaattaat ggacctggtg gcaaaaaaag
    10801 gaaaagccgg tggtggctat tgtacttata tcagcggaca tcgcgcgccg tttatttttt
    10861 cgaatttcaa tggtacgagt gccgatatag atgtgcttac acatgaggtg ggacatgcat
    10921 ttcaggtatt tatgagcagg ggttttgata cgccggaata tttttggcca acgagtgatg
    10981 ctgctgagat acatagtatg agcatggaat attttgctta tccgtggatg gatcagttct
    11041 ttaatggtga agcagataaa tatcgctatg cacatttggt tgagtcgctg cagttcttac
    11101 cgtatggcgt ggcagttgat gaatttcaac atcgcatgta tgcagagccg gagctgacgc
    11161 cattgcaacg taaaaatata tggcgcgaga ttgaaaagaa atatttgccg catcgcaatt
    11221 atgatggaaa tatattttta gaggaaggtg gcttctggca aacgcaggcg catatttacc
    11281 agtcgccgtt ttattatatc gactatacct tagcgcagat gtgtgctttt caattttgga
    11341 tgatggcaca ggaagataca acaaaagcat tcaacaatta tgtggaatta tgcaaggcag
    11401 gtggaagcaa atcattcctg gaattggttg cctatgcaaa actaaaatcg ccattcgatc
    11461 cacaagcctt cgcagatacc atcggtcata tcgacaacta cctgaaaggg gttgatgatt
    11521 tacagttgaa ttaacttttt tttcacgcag aggcgcagag cctaaactgc atttctccgc
    11581 gtctctgcgc ctctgcgtga accaaaccgt ctaaaatccc tattgcagac tactaaccct
    11641 ttccaaccac cagaagtaat tgaaattatt gtaaagtctt ttttcttcag gttgttgcga
    11701 caatgcttgt aattgttgat gaagtttttg cttgagtggt tttagcggtg agcgcatatg
    11761 ttgttttagt gcgcgcaaaa cagtggcata ggtagatgct tttaattttc tggattcaag
    11821 atgtcgattg aaactgacga ttagggaatc gatgagtagg atattcccta attcaaagtg
    11881 acaaagtatt tgcatcattc tgctatcggt gaacaagtcg attactgttt ctgcatcctt
    11941 catatccatt agcgtttgca gcgcatcaag gcattgatcg aattgtttat tgcagaatag
    12001 aacgtaagcg cataaataat acaaggtgat caaatttgga cgagcgataa atggtgcaac
    12061 atttgtaatt tgagcaagca cggcagttgc tacggataat gcgtgcacaa agtcttctaa
    12121 tccgacgtaa taattcaatt ctaataaata actcattctg aatacctggg catcaagatg
    12181 ttgcatgtgc cggaaggcat cttcttccgg tagtttacgc aaggtctgta tacctgcaat
    12241 cagcgcttca tgtttttgaa gaatgaggca gtcgatcaga taattattca atgtagcaaa
    12301 ataccgatcg gcatattgtt gtcgcaagta tggctgatca tccatcaatt gcaggaaggc
    12361 gctattatag tcgaatgccg tttccgtttg ttgttcggcg aaagcgtata atgccaaaac
    12421 ctgcatgcga tcgagacgcg cacggaaagt agtagctgag cttgcatctg caaacaatgg
    12481 tgatgctgcg agcgacttca atgcgtggtt atcgcgaccc ggaccgagtt ttttctcgat
    12541 atgcatttta tagatggcag cagagtttat ccaatattcc gaggtaatct ttatttgttc
    12601 aagacaatgc aattcttcgc gatgtaaagc tgcaaggccg ttgtgattat agtcggtgta
    12661 ttgtgtgcgt gcggctaacc gtttttctat cgcaataagc gcaatcacct cttcaaattt
    12721 ttcaaaggca tatccctttg ccttatattt ctttatttgc ttagcacaag cagcatatag
    12781 tccacgattt aataacaagg agcaataatg cacgccgagc gttagtttgt gcacttcatc
    12841 atcataggct tcaaaacgat gtaatgcctc cagaagggca tcgtaaagtt gttttttcag
    12901 caccggaaag tatgatgccg acttaccggt agcgaactgt tttgttaaag tttgttcccg
    12961 gtacttgtcc tgagcatcga tagcttcata aaggcgatgg taagtgctat ctttcgggaa
    13021 atgtcgggtg gcaaaaagct tgaaatagcg cttttcagcc atcgacatgg attttatcag
    13081 gtcaaaaagg gaagtatctt gttttttcac agacctctaa tgaaatttgg cttgaaatgc
    13141 ttgataatat tgatacaaat accaaaaatg tcctatcaga cctctaatat aagaaagata
    13201 tttgatttcc gtggaaacaa cggttggcgg agatttgtat ccgtaaacag aacgttatga
    13261 agaaaatgta taccatcatc gcggcgatac tcctctcgac cgcagttttg cctctgggtg
    13321 ctcaatcgtg ggtaaactat agcaatctca gctatgttac cgatgccgag ccgtctggca
    13381 cagatatctg gatcactgct aaaggaggtg tgcagttatt caatacccta accggcactt
    13441 caatgttttt cagaaagggt gatgcgggac taccatcctc atcagttgaa caggttgcta
    13501 aaagcaatgc caccggtgtg atctgggtgg gaacttatga tgccggagta gttgagtggg
    13561 atggcgattc gtggttgaca tatccatttc cttctgcaat gttgctctat cgcatgaaat
    13621 tcgatggatt cggaaatctg tggttgcaga ctgatgcagg cttatttcat ttcaatacca
    13681 ctgatcacgt ttacactttt gtgaactcaa caggtggtgc aggatgggat tttaatgcat
    13741 gggattttga tatcaccccc gataataaag taatcatatt tacaggcacc aattgtcagg
    13801 taatagatgc tgctactttg gcagcaatcg attcattccc caattcagat tcaccaaatg
    13861 tgctggcatg ctccccttca aatgtgcgcg tgtataatgt agatgaagaa acgtatttaa
    13921 ttaataacgg cttcgcgata gaatttgaat ttaaagacaa cactttcact cccgcaacag
    13981 acggtttgcc tgaattcgct ttcgtgcaaa atattattcg cggaaccgat ggagcattat
    14041 atgcttatgt aaataatgca tctgtttatc gcctcgatgg tctgacatgg acatatgttg
    14101 ataatgcagc tgtttatttc cctgaatttt tagtgtatac cgatggtgtg gaattttatt
    14161 tcagcagtaa taattatgca tctactcccg gagtaatcca tatgtcggaa acagattatg
    14221 tgaaaatatc ttcactggaa tatgcattta caagtaatgc cataaaaggt ttaactgaag
    14281 atgcagacgg caatatcttt atacttagtg gttcgggaca atatcaatac aacgagctaa
    14341 ccgacgattt ttcgctgttc ggaaatgtgc cttctattta tggtactatt tatgatctcc
    14401 gttctgtaaa tggtatattt tattgcgtga attatggcga cctgcttgaa tacaataacg
    14461 gtacttcatg ggtaaatatt ccttatgccg acggatatac atcgccatac atcttcgact
    14521 atgatgttac ttcagatggt actatttatt ttgtaaatga cgatggtgca ttcaaatata
    14581 aagacggaga aacaacctgt ttgttcccaa caccatcatt gtacgattgg ggattatctg
    14641 taacctatga tgccacacgc aatttattgt ggataggtcg catggatggt attttgaaat
    14701 acgacttcgt aacacaagaa cttatcgatg ccatggatgt gccggcaatg agtgatggta
    14761 tggccattca ggaaattaaa gtggcaccaa atggtgacgt atggttcgga gcaaataata
    14821 ataaagcata tatgtatgat ggcactgagt ggactgatta caatgttggc acgggtatgg
    14881 gatttgtgac ttcatttgat tttgaaggag ccaacattta tttcggtatc accgatggga
    14941 atggtggtgt gcatgtatgg gataccagca atgataattg gtcatggtta agttctgcaa
    15001 ccgatccaac tttagtatct gatgaagtca gctacatgac agtaactaca ggtggcgact
    15061 tatggatagc gcatgtgaac gcaggtgtaa gcagattgga cggcactcca tctgttggta
    15121 tagatgaggt tacagcaaat aatgcaatca tttatccaaa tcctgttgcg cagacattat
    15181 ttgtatcagg tttggaagat ggaaattatc agtatgtaat tagtgatctt tcaggaaaac
    15241 aattatggtc atctagccaa gcgactcatt ccattaatgt atcgcaattt ccttccggta
    15301 tttatttgtt gcaagtgcgt gccggagata aatcggtatt taacggaaca tttattgtgc
    15361 cgaccaaata gccacatcaa ctcctcaata aaaaagcccg atgtatttct gcatcgggct
    15421 tttgttttaa tattattaac ccctaaataa accgtgtgag gaggtttgag gaattaaatg
    15481 ttcgcggttt cttcggcatt cagtcgaaat ccgttgccat gaatatttac gatttcgagc
    15541 gttggatcat ctttcagata tttgcgcaat ttcgttacga aaacatccat gcttcgtgct
    15601 gtaaaatagt catcgcggcc ccagatttgt ttgagggctt cttcgcggta aacgatatca
    15661 tttactttca tgcaaaacag tcgcaataac tccgcctctt taggtgaaag tttggcctcg
    15721 ctatcaccat ttttgagcat gcgtgttttg tagttgaaat gatattttcc aacattgaat
    15781 tctttctgct gattgttggt tctggctttg cctccgcgtt ttaatacggc ggcaatgcga
    15841 tagagcagtt cttcgctgtt gaacggtttg gtgatgtaat catcggcgcc ggttttgaat
    15901 ccttccagta catcatcctg caatgttttc gcagtgagga aaattacagg ttgatccgga
    15961 ttaacgcgtt tgatttgtgc ggcgagtgaa aaaccgtctt ttttgggcat catcacgtcg
    16021 aggacgcaaa gattaaattc ttccttaaaa aacagatctt ctcctttttc gccatcggtg
    16081 gccagacgta cttcgtaacc attcatggtc aggtagtctt ttagtacggc cccgaaatta
    16141 gggtcgtctt ccaccaacag aattttggtt ttgatctctt ccatagtagt agtatgcttg
    16201 ttttattgat gcggtaaaaa aatcctgaat tgtgaacctt tctttaattg acttttcacc
    16261 tcaatggaac ctttgtgtgc ctcaacaaca gttttcacat aggttaatcc gaggccaaag
    16321 cctttgatat tatgtacgtt tccggtgggg actctgtaaa acttctcaaa aatcatgctc
    16381 atatcatcat tgctcatacc aatgccgtta tcctctacgg tgatataaat gcccttattg
    16441 tcgctgcgtg tgcgaacaac aatatgtgga tgttcaggcg tgtatttatt ggcattgtcg
    16501 agcaggtttg agattacatt actcaaatgt acttcatcgc cggtaatttc aaatctatcg
    16561 gcaagtaatt ctgtttccac agtgccgccc ttttcttcca caagcaggct gatattttcc
    16621 acagcacgat aaattatctc atgaatgtca acatcatcat tgctgatact tacctgatgt
    16681 ttatcaagca gtgccatttg caatactttt tcgacctgtg cattcatacg tttattttca
    16741 tcgcgaataa tgccggtata gtatttcacc ttttgctgat cttgcaatat catagggtta
    16801 tttacggcat ccacggctaa agagattgtt gccaacggag ttttaaactc gtgggtcata
    16861 ttattgatga aatcattttt gatttccgac atttttttct gttttacaag aataaatatc
    16921 gtgtaggtga atacggcaac aataatggta gtgaataata aggaacctaa tagcaaggac
    16981 cagatactgc tcaggataaa gcttttttga tgggggaagc ttaccagcag aacatcgttg
    17041 ttaaatacaa tatcgtcggg gaacagactt actttgtgcg atgatgctgc aagtttctcg
    17101 aggccgaaat tatcttcggc attgctgata aagaatttat catgcatgag tacaccaaaa
    17161 ttaaaatcgg tggtgatacc gcgattattc agctcgtgat ataaactgtt tttaagaaaa
    17221 ttcgtgtcga ttgtttgctc gggagtgata ccacgttgta ccatgcgtat catcatttgt
    17281 tccattgcct tttttatgcg accggaattt aattgcaatt gcctgtcgat gtcggacaat
    17341 atttgagata ccagtgcatc attgggtgat gaattatcgc cgattaattc cagcggctcc
    17401 agaatcatgg taggcttttg agaacccggc attccttgca aggcatcggc aaaaggctct
    17461 attgttgtat attccggatc attataatat gcctgattgg agcgaagcga ttccagcata
    17521 cctgccatgg tgctgtcgct caacacattt tcataagtag tcaaagaatc gcctgtgccc
    17581 atccagtagg tggagtcgga aaaaaacaaa ttcatctgac tgctgagcac cgaggctgca
    17641 atatttgtct ctaccttgtc ggcaacccga ttaagtgcct catttacatg gtaattgaag
    17701 atttgttcct tctgttcaat agtgttttga atccagtata cctgcaccag gatgatgccc
    17761 agcagcgccg tgctcatcga aattacaatc caccatatcc ggtttcttaa cattccttac
    17821 aaaactacat ttgttaaaat tgctgcggcg acgtttaacg atacattaac atcttttatc
    17881 aggctcttaa ccccaacggc acttgtttca gattacattt gtcatcgtat cgtccggaaa
    17941 tgaccccgga gcagtaatac aacacaaacc aatacaagta tgcaacggtt cctttttagt
    18001 atcactacgc tcataatacc ggccatgttg tttgcgcaac agcctgataa tcagatacat
    18061 ctgcgcattg atgccaatgt tgacggccag caggttaaaa ttgacacctt catagaatcg
    18121 cttggcgacc tcaatctgga cgagttctta cagggtttgg gactggagaa tgagctcaat
    18181 cagctgaaca ttgacatcaa cagtggtttc ggcgtcatgc cgagttttga tgaggaggct
    18241 atgcaagaga tgttgaagag cttgagtgag ttggaaatgc ctgagatgcc tgaattacct
    18301 gagttaccgg gaattgatat gggggaagcc atgtcgtttg ggtatgcggg agaaaataga
    18361 gcatttttgg gcgtatacac aaataaggta actgagggtg cgcagataag tgatattgtt
    18421 gaaggaagtg ctgcggaatc tgcaggattg atggaaggtg acatcataac gcatattgat
    18481 gcaagaagta tagaatcacc ggcaaacctt atggaagtat tagccttgta ttatcccggt
    18541 gatgaagtaa cggtaactta tatccgtgca ggtaaaacag ataaagtaaa acttacccta
    18601 caggaaaata caaatgcatc aagcacatgg ggctcgcaga actacaattt taattttgat
    18661 ttccctgatt caatttttat gggccagggt aattatttaa tagaaacacc ggcgcatggt
    18721 tatttaggcg tttatctgga agatgctgat ggcaaagttt tggttacagg aattgaaggc
    18781 ggatctgctg cagaaaaagc cggtttgaaa gctgatgata ttcttcttga aataaaaggc
    18841 aacaaagtgc aatcgtatga tgaagtgtta gagatcatgc aagaaacaaa acctgatgag
    18901 aaaattcaca ttatttatga acgcggtggt aaacgcaaag aaacagatgc aacattaaaa
    18961 tcggcaggcg gcggagcata ttatttcaat tttaataatg atgaaggata caataatgat
    19021 attcgcatag cgccatgttt gccaacgcca cccggtagtt catacagcta ttattcacgc
    19081 gatagctcaa aaacaataaa tatttgcatc accggagcgt atgcgggtga ttatggcgat
    19141 agcaccggag gatttttgcc ggatcaggca catccactta tggatcctaa caatctcgct
    19201 gtatatgcca atccgagcga tggcactttc cgcatccatg tagaccttcc tgaggaagga
    19261 gatgcaaaag ttgtcattac tgatatggca ggaaaagaag tttataatga attgctcccg
    19321 aatttctcag gcacttttga tcgcacagtc acattatcga atgcgtcaaa aggcacttat
    19381 tttgtgaagg tgacgcagaa aggttattca agtacaagaa ctgtagttat tcaataaaag
    19441 gtttaagggt ttaaaacaaa aagcgcatca aaataatggt gcgctttctt tttgtgatca
    19501 tttttccggt aatcattttt ttgttttcgc ctcattcact ttaatgcgat gttttactat
    19561 gcgcgtgatg agtgtttttg gaaggggttt atcataaggg aattgaatgg taccttttga
    19621 tgttttatag tcttttaatt cctctgcaaa ttctgcaaca ccttctcccg ttgggaaaaa
    19681 gccaatatgt tgtttgaaac ctccaaaata aacaagcggg ccattcaatt tatatccggg
    19741 catgccatag ctgatgcctt cctcggcttt aggtgctgtt ttttttatta gcgcacggat
    19801 ttccatgagt tttaattgga tgtccttagg aaaatcggca atgtagcctt caacagatgt
    19861 tgcttttttc attttcatga tagcagatta tatatacaaa tatacacgtt gtattaattt
    19921 tcctgtaagc atttattaaa gtccaatgag aatttttcag gtttaaagtc agaattattt
    19981 acttttgaac aggcaatcaa agaaaaacat gaagaaaaaa aatattattt attgggtagt
    20041 aacggggatc gttgcagctt atatggtatt cagcgcactt cctgatgcac ttttgcagga
    20101 caaggcggtt gaatttatgc aaggactggg ttaccccgat tattttaccc gcttcatcgg
    20161 catcgctaaa ttgttgggag ttgccggaat attattgccg cttccattac gactaaaaga
    20221 ttgggcttat gcaggtttgg ttttcgatct ggttggggcc tt
//
LOCUS       DAOQHY010000045        19903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1228_length_19903_cov_6.943218, whole genome shotgun sequence.
ACCESSION   DAOQHY010000045 DAOQHY010000000
VERSION     DAOQHY010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 19903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19903
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1228_length_19903_cov_6.943218"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PLL28_05455"
     CDS             <1..329
                     /locus_tag="PLL28_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68801.1"
                     /translation="NAFISPDGNILIYTKYNAPGGFGSGDLYIAKKDNGGNWQEPQNM
                     GTDLNSMYMEYCPFYHYPTATLYYTSRRCNLTPKTFNDVNELQQYISGNENGNSRLYY
                     RKMKIE"
     gene            complement(530..2650)
                     /locus_tag="PLL28_05460"
     CDS             complement(530..2650)
                     /locus_tag="PLL28_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNF68802.1"
                     /translation="MKKPQIWIALLFLALSSCSDHTEYTKVNTRFSTTDPFAKTSAPS
                     KFVEFSAEIDTVLAGENGLIIVIPKGALLNAKNEVVQGKITMELFQANGLNELVFSNL
                     TGNSSSGQLSGGSAFYINFTKDNEQLHIDQNNPITISTPKTAKEMLMYSGNRDSVGNM
                     DWVDPIAPEKYLDPVPLSTLNFAPEIFDNGLLDILPFEGKNKFVRSFSDSLYYSVWLV
                     HKKLSSFTPDQGEKFLLEPNGKKFDLKDIQKSHQSGLTDTSSDDEYSDCGIDPIKVKT
                     IKENAFENTFIATKEFETRMQAIHRTFDQRVLDLYVNNTDKQLWVVDSMASQLLGNAA
                     DREVFLKFSREKLTNVRDVEKNPSAKALARFYQKRSEGLTREMDTVENALLKKYKLLQ
                     KDYDKELAKYVEVLRKREAHRMLYFNYTLTSSGWKYFAIPPEFGRTFREKKLIGRLTD
                     SQKFDMVNVYVWMPENTSLMRLTETEKNVFISKSMRAVETTPVLDNTKLQFFAVAVIG
                     EQYFFDEVKLSSEEEREVTATFELKPTEKAKIDAVLGNQEGKADLNNSISRDLKFQTK
                     RLAFNKAADKYLEKRTKYDLLFSTTVNNCCRMSSKSIQVGNALFNVNCTRCHLAERGV
                     KLTGPAFDNLLDHSSGQYAYFSAFVRDSRALINSGDDRATTVFNEYRSVMPAFEDLKE
                     EEIMSIYNYLIATLSCSNQPTILH"
     gene            complement(2713..6000)
                     /locus_tag="PLL28_05465"
     CDS             complement(2713..6000)
                     /locus_tag="PLL28_05465"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNF68803.1"
                     /translation="MNRKFVAYWIVSALVAYSPTFAQTTLFSRLSASQTGVAMHLDLQ
                     ETATMNVLLYQYLYNGGGVAAGDVDGNGYCDLFFTGNLGQDKLYLNQGNMQFVDATRQ
                     ANILPPGGWSSGVTMADVNGDGFLDIYVCKSGQFSTRDRHNQLYINNGKGAFVESAKK
                     FGLDDSSFSTQAAFFDMDNDGDLDMFLLNHAVTQLHGFNIGDVRNRRDKYAGNKLFRN
                     DNNYFTDISAEAGIFGTPSNFGLGLMIGDVNNDAYLDIFVTNDYNEQDFLYINNHNGT
                     FSQVLQDAFGHTSNFSMGCDMADINNDGWLDLLVMDMLPEDPERQKKLKGPTKFDAYQ
                     MSADYGYFYQHMFNTLQINNGNNSFSEIAWSAGVAATDWSWAPLFADYDLDGIQDLYI
                     TNGFRHDFTNLDFLKYTYSEAEQKAFDAGEKLNTLDLVYQMPDVPLSNYCFKGSDSLQ
                     FKNVTVDWGVQIPSYSNGAAYADLDNDGDLDIVVSNIDDTVFIFKNNARELKQNHYLK
                     IKIIGEGKNTFGVGTMISVAIGNQSYKRQLMPTRGFQSAVEPVLYFGLGESKNVDRIQ
                     IIYPSGKEQILSNISADTTLIVYEKNGQYELWAADYLPEFIDVSNKVIPYSVSEEPAD
                     DFKKEPLIPYVYSDQGPALAVGDANNDGISDVFITGGKNKKSRLFLGSDEGFIPSFGG
                     VWERDSAYEDVDAAWIDVDNDGDSDLFVVSGGNEYPAGSRYYQSRLYLNMSNGRYVNA
                     NQLLPESLQAHSVVRTCDFDNDGDMDIFLGGGVVAGSYPLSSPSAIYENIGGKFMDIT
                     ARICPELNTIGLVQDAQWADIDADGKQDLVVVSHWSPLRVFINGKKKFIESTVPSGLS
                     DYSGWWNAINIVDVDLDGDMDIIAGNRGTNHPLKASKEFPAHMYVCDFDRNGKIDPII
                     TTFDKSGKEIPIASKDDMLEQIPALKNRYVYYADYAKASIFDLFPDVNWDTITHFTAT
                     DFNSSIFINNGKGSFTRQLLPEQIQWFPVYAIAVKDINRDNYPDLILAGNQFGTRPEL
                     TRLDAGYGACLFGDGKGGFTFKNAAETGLFIDGEVRSMCLTKLNREKYLLVARRNASV
                     MAYLLNK"
     gene            6226..9177
                     /locus_tag="PLL28_05470"
     CDS             6226..9177
                     /locus_tag="PLL28_05470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNF68804.1"
                     /translation="MMHVYALIRRAILPVVLLMPVLIWGQAPTLTGVISDQSGATLPG
                     VTVLISGTGKGTISDFDGSYVLAFDAAGTYTVNYTYIGYKAVTETITLTDGQNLKKDL
                     IMQEDALMLETAVVVGYGTIKSKDLTGSITNVGVKDFQGGNVTTPEQLITGKVAGVQI
                     TSNSGMPGSGSRIRIRGGTSLNASNDPLIVIDGVPVDNNGISGSGNALSLINPDDIEN
                     ITVLKDASAAAIYGSRAANGVIIITTKKGFSDNKLHIDVSSTSSLSQVTGYVDVLTAD
                     QLAAVLDTAGTATQQALLGSENTNWQKELYRMGFAEDLDLTFTGGIKNLPYRLNLEYM
                     NESGVLDRSQLGRMGASLGINPTLLNGKLKTEANGKYYHTDNFFADQGAIGSAITFDP
                     SQAIYDDASVYGGYWEWTNTSGGLIPLAPKNPVGLLNQRDDESNVNRFIGNIKLDYEI
                     FKDFHGVINGGIDMARSNGTVVVPAEAASQYPNGSNNQYEQSKDNKLLEAYGNYKKDI
                     KSIKSRIDFTAGYSYQNWLTQSPSFPSLSFAGDTITPVGIDFETENTLISFYGRLNYN
                     LKERYMVTATLREDGSSRFSPDNRWGLFPSVAAAWLVSDEAFMSNTKMYLKLRAGYGV
                     TGQQDIFYDYPYIANYDSSTSTAQYQFGDAFYYLLRPDGYDPNIKWEETTSFNIGFDF
                     GLKGDKISGSVDLYKKLTNDLLATIPIPAGTNFTNQILTNVGSMENTGVEINLSYIAL
                     DNKDMSLEFGINGTANRNEITNLTLVEDTSSAGILVGGIAGGIGNTIQIQSVGYASNT
                     FFAYQQQYDENGNPIEGSYVDQNGDSIINADDLIWGENPTPDFYLGFSAAFKYKKWSV
                     STVLRGEFGKYMYNNINSTRGIFQNVPSASYMQNLSTNYLETGFQSYQLLSDYYIENA
                     SFVRMDNFAVGYDVGRVFNDNASLTVGAVIQNVFVISPYSGLDPEIAGGIDNSIYPRP
                     RIYSLNINLKL"
     gene            9189..11000
                     /locus_tag="PLL28_05475"
     CDS             9189..11000
                     /locus_tag="PLL28_05475"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNF68805.1"
                     /translation="MKNKILIAVTALAISGFTLSSCFNDLDLAPPSGINAETVYNNPD
                     NYVHVLAKLYAGLSISGNQGPAGNPDIAGIDEGFSQYIRVLWNLQEATTDAAKCAWND
                     PGIPELNKMQWSSTNSFVNAMYYRIYFQIPLCNEFIRESADAKMSERGFSDAEQTTIR
                     GYRTEARFLRALSYYHALDLFGNVPFITEDDVVGAFYPERITRAELFNYVESELLAVA
                     TELPAPRANEYGRADRAAAWTLLARLYLNAEVYTGTARYTDCATYCQKVITEGGYSLE
                     PVYDHLFMSDNQGSNEVIFPVTFDGQFTQTYGGTTFLTHSTVGGDSLSQIDKAYYGIE
                     FGWGGNRSTPEYASFFPDANDARNMFKTFGQTLEFTEDAQLGQFVVGYPIAKWRNVLS
                     TSVAEFTATISGDVMTVTSVADGSVQVNQNLAGSGIAGENKIIEFITGTGGTGTYRLA
                     NADNVTSPTTIHAAIPGTNLDAGGNYTDIDFPMMRLADVYLMYAECAARGAGDAGLGV
                     EYVNMLRERAYGDVAHNVGSITPESVLEERARELQWEGYRRTDLIRYGLFTGGSYLWA
                     FKGGPFNGTSVPETLNLFPIPAADVVANPNLVQNPGY"
     gene            11128..>12185
                     /locus_tag="PLL28_05480"
     CDS             11128..>12185
                     /locus_tag="PLL28_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNF68806.1"
                     /translation="MKIKNLLLICFLACTSVISAQQIDQICPPNWFIGMRDSSVQLLI
                     HGTGIGNYDLKINYPGVRLVAVTRVENKNYLFADLVIDKTARPGAVDLYFSAGKKSFT
                     KQWALQARAPWTNTYGLDAGDLIYLLMPDRFANGDTTNDVVASMNETISDRKDPKGRH
                     GGDIAGIMQHLDYISKLGATAVWCTPLVENNEPKWSYHGYAITDHYKIDPRFGNNQQY
                     AEFVQSAHKHNLKVVVDLVHNHVGDQHWFIKDLPMPDWIHQWDTSFVRSNYRTSVKND
                     PYASAYDVKKMNEGWFDKHMPDLNQENPYLAKYIIQNNIWWIETYHVDGFRLDTYPYS
                     DLSFLQDWGVAIQKEYPG"
     assembly_gap    12186..12285
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12286..12600
                     /locus_tag="PLL28_05485"
     CDS             <12286..12600
                     /locus_tag="PLL28_05485"
                     /inference="COORDINATES: protein motif:HMM:NF021922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclomaltodextrinase C-terminal
                     domain-containing protein"
                     /protein_id="HNF68807.1"
                     /translation="GKEKEIFNYVSGLANWRKNSDAIKYGKLMQFIPENGVYVYFRYT
                     EKETIMIVLNSASTEYTLDTKRFAERIGNFTIAQDVVTKQNNTIAGLKVAAKSPGVYV
                     LK"
     gene            complement(12640..13797)
                     /locus_tag="PLL28_05490"
     CDS             complement(12640..13797)
                     /locus_tag="PLL28_05490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68808.1"
                     /translation="MINYRTLCLIIGINFSAFFAQSQNATFEVTLGGFDAEAEAAFNY
                     ATDQWSQYLISDVPIKVHANLVTLLPGQLGITFPNGEKNFVGAPFNDTWYASCLANAI
                     TGTELNPGYADMDIFINSTANWYFGTDGNTPAGQYDFVSTVMHEICHGLGFLSLSNKD
                     VTTGSFGLIYATDFFPLVTSFPWPELDTLPSAMDHYLSNGSGTMLLTFENPSDALGDE
                     FTSNNIFCISEKVLNAHGGEAGRIYAPSTFTLGSSMSHWNEGSYPVGNENEFMTPNAA
                     SGHSSQVPGPMTLALLEEIGWEIDYTIDINTTIQPDESPIIFPNPANENAVISNLPVN
                     ASWDVVSLDGRNVASGANNVIDCKDWARGIYIVRFISDRGAEIKSSKLVLN"
     gene            13921..16431
                     /locus_tag="PLL28_05495"
     CDS             13921..16431
                     /locus_tag="PLL28_05495"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HNF68809.1"
                     /translation="MNFGFQYARALVVMGFCLITSSLFAQNRTINGNVRDKKTNESVL
                     FAGIYLLHTETAAQTDEKGNFSLIAPSGYDTIIVTSIGYITDTIVLGRELTQQLNVAL
                     DQSLYELNTMVITAQEEPAKTIMRKVIENKKINDQQHLAAYKTRGYNKIEIDLGNLEM
                     LTDYDSIPVDTTKKEKKPNPYAVLLDHIDSTSDVTHFLPFFFTETVSDFYYRKNPKTR
                     KEIIIASRTSGINNASATQILGNYYQQFNLYDNYWFLLSKNFVPPITDAWNFFYRVDL
                     ADSAWIDNDWCYLIRFQPRQQQENTFHGFMWIADSSFAVKEISMQLDSAVNINYCKRA
                     QFYMLFDHYNDSAWVLKEDRLVVEFLPIKNSTSFIGRRTTYYDDYNFDPNVFEEVVKY
                     KDDIVYTDSVVNNDEAYWRSVRPDSLTYNETGVYELVDSLKNIKSFMTVVDVYNTLMY
                     GYWNLGYVRIGPFANMASSNDVEGFRVRIGGKTGDLISKRLTIGGYGAYGFQDRTFKY
                     GAEFHAVIAKKPWQEFDLKYYDDLDVSNNDNVTFGEDNLLSGFYRRRDTPQKIVDIEK
                     FQVSYKKDWIWGLSNTITLTSTHMHPLFDIYYYDNDSLRSNITNSEIKFGLRFAYREK
                     FMFDNYRRYSLGTKYPKLELYYRIGIPDLMESDFRYTAFEVNIWDFFTVGSIGGTSYT
                     LKAGKIFGTLPTLLLETPPGNETYFMNHANFNLMNEYEFVNDTYAELFLTHSFYGFFL
                     NKIPYVNKLKLREVATFKLAYGSLSDANVELNDYLKNSAPYYIGNTSMTNLPYMEAGI
                     GVENIFKLIRVDAIWRLTYRNNPLAPNFGVRLGVNLDI"
     gene            complement(16480..19269)
                     /locus_tag="PLL28_05500"
     CDS             complement(16480..19269)
                     /locus_tag="PLL28_05500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNF68810.1"
                     /translation="MNLRLTLCSIVTLMAFTLSAQLISTDPIFPTPDGPVTVIFDASQ
                     GNAALAGYTGDVYAHTGLITQDSDTPSDWQYVQGVWGTADPEVLMTSLGDDKYSITFS
                     SIMDFYGAAADDTIYQMAFVFRNAAGTIVGRDVDGSDIFVNVYAPGLNVAITVPDYNP
                     YLVNPGDNVHIEASSTYSDSLYLYINGVEIIAGEDSNITYDLTAVDPGLSDIVVKAVS
                     GADVMYDTTYFYVLGTVTTEAVPAGMHQGINYIDDNTVTLVLYAPYKNYVFAIGEHSD
                     WLLTDETFMKRDPDGATYWITLSGLEPGKEYPYQYFIDGNLTVADPLAEKVLDPWSDG
                     YINEATYPGLIDYPTGKASGIVSVFQTAQPDYVWAVPDFTSPPTEKLVIYELLIRDFI
                     AAHNYATLTDTISYLKTLGVNAIELMPIMEFEGNESWGYNPSFFIAPDKYYGTKEALK
                     IFIDSCHANGIAVILDIAMNHAFGQCPLVQMWWDATANAPALNSPYFNQVPTHDYNVG
                     YDFNHESDATEAFRNIVFTHWITEYHVDGYRFDLSKGYTQTNTLGNVAAWGAYDASRI
                     AIWENIGSVIWAVNPDAKLILEHFADNSEEETLSNAGFMLWGNMNYNYNQGTMGYGGS
                     DLNWASYQSRGWDDPHLVTYMESHDEERLMYKNLTYGNTTNPDYNVKNLNIALSREEL
                     AGAFLFTIPGPKMLWQFGELGYDYSIEYGCRVCNKPIRWDYYSNANRLRIYQVWSELI
                     KLKTTYPAFSTTNYNMSVAGFGKRINLNDPDMNVTVLGNFDVNLANISPNFQHAGWWY
                     EYFSGDSLNVSDVLASFTMAPGEYRLYTDVRLSTPDIIEGINHVDEMANNSIIAMPNP
                     TEGETEILTTLQTADDVVLEFYNNTGSMIERYDLGKQAEGIFSFTWNSNAPAGIYFCR
                     LVYGDNAGVVKISKL"
     gene            complement(19505..19813)
                     /locus_tag="PLL28_05505"
     CDS             complement(19505..19813)
                     /locus_tag="PLL28_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HNF68811.1"
                     /translation="MEKKWYASALGRFRVVAILEGISFLLLLGIAMPLKYAANIPEAV
                     EYAGWMHGLLFISYMFTLLQVKIEHEWTFGKAALAVLASILPFGPFILDKRILQRESA
                     "
BASE COUNT         5833 a         4254 c         3910 g         5806 t
ORIGIN      
        1 tcaatgcatt tatttcaccc gacggcaaca tcctgattta tacgaagtat aatgctccgg
       61 gcggatttgg cagtggcgat ttatacattg caaaaaaaga caatggcgga aattggcagg
      121 aaccacaaaa tatgggcacg gatctcaact ccatgtacat ggaatattgt cctttttatc
      181 actacccaac cgctacgcta tactatacaa gtcgccgatg taatctgaca ccaaaaacct
      241 ttaatgatgt caacgaattg cagcaatata tcagcggcaa tgaaaatggg aatagtcggt
      301 tatactatcg gaaaatgaaa atagaatagg gattaaacca tctcgtcaaa attaatttaa
      361 actcggcgga tcggagcact tcaatgatct ggttatataa ttataaatcg acattaattc
      421 ttcttcagtc agttcctgat atgccggcat taccgattgg tattcgttgt atacattatt
      481 ggcgcgctgg ttgccgctag ctatctgtgc ttgtgaattg cgaacaaact caatgaagta
      541 tagtcggctg attgctacag gataatgtgg ctataagata attataaatt gacatgattt
      601 cttcttcctt cagatcctca aatgcgggca tcacggatct atattcatta aatacagtag
      661 ttgcccgatc atcaccgcta ttgataagcg ctcgtgaatc acgaacaaag gcgctgaaat
      721 aagcgtattg tcccgatgaa tgatccaaaa ggttatcaaa tgccggtccg gttaacttaa
      781 cacctcgttc agccaagtgg cacctggtac aattcacatt aaaaagggcg ttaccaactt
      841 gtatagattt tgaagacatc ctgcaacaat tgttcaccgt tgtagaaaac aagagatcat
      901 acttggttct tttctccaga tacttatcag cggctttgtt gaatgcaaga cgcttggttt
      961 gaaatttaag atcacgagaa attgagttat tgaggtctgc cttaccttct tgattgccta
     1021 gcactgcgtc gatcttcgct ttttctgttg gtttgagttc gaaggtggcg gtgacctccc
     1081 tttcttcttc ggacgacaac tttacttcat caaaaaaata ctgctcgcca attacggcta
     1141 ccgcgaaaaa ctgcaatttg gtattatcca ataccggggt tgtttcaaca gctctcatgg
     1201 atttggaaat aaacacattc ttttctgttt ctgtaagacg cattaaggat gtgttttccg
     1261 gcatccatac atacacattc accatgtcaa atttttggga atcagttagc cggccaatta
     1321 attttttttc tcgaaaggtt ctgccgaatt cagggggaat agcaaagtat ttccatccgg
     1381 aacttgttaa tgtgtaattg aaatacaaca tgcgatgcgc ctctcgtttt cgtaatactt
     1441 caacgtactt tgcgagttct ttatcatagt ccttctgaag caatttatac tttttgagca
     1501 aagcgttttc taccgtatcc atttctctgg tcaacccttc cgatcgcttt tggtaaaatc
     1561 ttgccaaagc ctttgccgac gggtttttct caacatcgcg cacatttgtt agtttctctc
     1621 tgctaaattt caaaaacact tccctgtcgg ctgcattccc taacaattga gatgccatag
     1681 agtcaaccac ccatagttgc ttatcagtat tattcacata aaggtctaat acccgctgat
     1741 caaaggttcg gtgaatggcc tgcatgcggg tctcgaattc ttttgtagcg atgaatgtat
     1801 tttcgaatgc attctccttg atcgttttta cttttattgg gtctattccg caatccgaat
     1861 attcatcgtc agacgatgtg tcggttaagc ccgattggtg gcttttttga atatccttca
     1921 gatcaaattt tttgccatta ggctccaata aaaatttctc cccctgatca ggtgtgaatg
     1981 aagaaagttt tttatgtacc agccacaccg aataatacaa gctgtcggaa aacgatctga
     2041 caaatttgtt ttttccttca aagggtaaaa tatcaagcag gccgttatcg aatatttcag
     2101 gggcgaaatt caacgtgctc aaaggaaccg gatcaagata tttttccggc gcaatcggat
     2161 cgacccaatc catatttcca actgaatctc tgttaccgct gtacatcaac atctctttag
     2221 cagtctttgg agtgctaatg gtaatggggt tgttttgatc gatatgcagt tgttcgttat
     2281 cctttgtaaa attgatataa aaagcactgc caccgctaag ctgccccgat gaggagttgc
     2341 ctgtcaaatt gctgaaaacc aactcattaa gcccattggc ttgaaataat tccatggtga
     2401 tctttccctg caccacctca ttcttggcgt tcaagagtgc acccttaggg attacaatta
     2461 tgagtccatt ttctcccgct aacaccgtgt cgatctctgc agaaaattca acgaacttgc
     2521 taggtgccga tgtttttgca aatggatctg ttgtagaaaa acgcgtattg accttagtgt
     2581 attcagtgtg gtcagaacac gaggataaag caagaaacaa aagtgcgatc catatctgag
     2641 gttttttcat gcggagtggt atcaacaatc taagatagca ttcatttatg cgatctgtgc
     2701 gcaacttgcc aattatttat ttaacaagta ggccatcaca ctcgcattcc tccttgctac
     2761 caacaaatat ttctcacgat tgagttttgt gagacacatg cttcgcactt ctccatcaat
     2821 gaacaggccc gtttcggcgg cgtttttaaa tgtgaagccg cctttgccat caccaaacaa
     2881 acaggcgcca taacccgcat ccagtcttgt caattcaggt ctggtgccaa attgatttcc
     2941 cgccaggatg aggtcggggt aattatcgcg attaatatct tttactgcga tggcataaac
     3001 cggaaaccat tgaatttgct ccggcaacaa ttgtctggtg aacgagccct tgccattgtt
     3061 tataaaaata cttgaattga aatctgtagc cgtaaagtgc gtaatggtat cccaatttac
     3121 atcgggaaac aaatcgaaaa tggaagcttt cgcataatct gcataataaa catatctgtt
     3181 ttttagagcc ggaatctgtt caagcatatc atctttcgaa gctataggaa tttctttacc
     3241 acttttgtcg aacgttgtga taatgggatc aattttacca tttcgatcaa aatcacaaac
     3301 gtacatatgt gccggaaatt cttttgaggc ttttaatgga tgatttgttc ctctgtttcc
     3361 tgcaataatg tccatatctc catccaggtc gacatctaca atattaatgg cattccacca
     3421 accgctgtaa tctgacaatc cggatggcac tgttgattca ataaatttct ttttcccatt
     3481 gataaaaacg cgtaatggcg accagtgcga taccaccact aggtcttgtt ttccatcggc
     3541 atcaatatcg gcccactgcg catcctgtac taatccaatg gtatttaatt ccgggcaaat
     3601 acgcgctgtt atgtccataa attttccgcc aatattttca tatatggctg acggagaaga
     3661 caatggatag cttcccgcta caacaccgcc tccaagaaaa atatccatgt cgccatcgtt
     3721 gtcaaagtca caagtacgca ctacagaatg cgcctgtaaa ctttcgggca acaattgatt
     3781 cgcgttcaca tatctgccat tcgacatatt taaatataat cggctctgat aatatcgaga
     3841 acccgccgga tactcatttc cgccgcttac tacaaagagg tctgagtcgc catcattgtc
     3901 aacatcaatc caggcggcat caacatcttc gtatgcagaa tcacgctccc atacgccacc
     3961 aaatgacggt atgaagcctt catcacttcc caaaaacaat cgcgactttt tgtttttgcc
     4021 accggtgata aatacatcag aaatgccgtc attatttgcg tctccaacgg ctaaagccgg
     4081 tccctgatct gaatacacat aagggatcaa tggttctttt ttaaagtcat cagcaggttc
     4141 ttcgcttaca ctgtaaggaa tcaccttatt gctgacatca ataaactcag gcaaataatc
     4201 agccgcccat aattcatatt gtccattttt ctcatagaca attaatgttg tgtcggcaga
     4261 aatgtttgac aaaatttgtt ccttgcctga tgggtatatt atttgaattc tgtcgacatt
     4321 tttagattca cccaatccaa agtacagcac cggttctacg gcagattgaa agcctctggt
     4381 gggcataagt tggcgtttgt atgattgatt acctattgca acagaaatca tagtgccaac
     4441 tccaaatgta ttttttccct cgccgataat cttgattttt aaataatgat tttgcttcag
     4501 ctcacgtgca ttatttttaa aaataaatac agtatcatca atattgctta caacaatatc
     4561 aagatcgccg tcattatcta aatctgcata tgcagcaccg ttagaatatg atggaatctg
     4621 aacaccccaa tcaacggtta catttttaaa ttgcagcgaa tcgcttcctt tgaaacaata
     4681 atttgataaa gggacatcag gcatttgata taccaggtcc aacgtgttta acttttcacc
     4741 tgcgtcaaaa gctttttgct cagcttcact ataggtatat tttaaaaaat ccagattagt
     4801 aaaatcatgt cgaaacccat ttgtgatata aaggtcctga ataccatcca gatcgtagtc
     4861 ggcaaacagc ggcgcccaac tccagtctgt ggctgcaacg cctgcgctcc aggcaatttc
     4921 agaaaatgaa ttattgccat tatttatttg caacgtgtta aacatgtgct gataaaaata
     4981 tccgtaatcg gcagacattt gatacgcatc aaattttgtc ggacctttta attttttttg
     5041 tcgttccgga tcttcgggga gcatgtccat taccaggaga tccaaccagc catcattatt
     5101 aatatcggcc atatcgcatc ccatcgaaaa attagaggtg tggccgaatg catcttgcag
     5161 cacctgcgaa aatgtgccgt tgtgattgtt gatatataaa aaatcctgtt cgttgtaatc
     5221 gtttgtcaca aatatgtcga ggtatgcatc gttattgaca tcgccgatca ttaatcccaa
     5281 tccgaaatta gatggcgtgc cgaatattcc cgcctcagca ctgatatcgg tgaaataatt
     5341 attgtcgttt ctgaataatt tatttcctgc atatttatct cttctgtttc ttacatcgcc
     5401 gatattaaat ccatgtaatt gtgttacggc atgatttaat aaaaacatat ccagatcgcc
     5461 atcattgtcc atatcaaaaa aagcagcctg cgtgctgaac gatgaatcgt cgagtccgaa
     5521 tttttttgcc gattcaacaa atgcaccttt tccattattg atataaagtt ggttgtgtct
     5581 gtcgcgggta gaaaactgtc ccgacttaca cacataaata tcgagaaagc catcgccatt
     5641 cacatctgcc atggtcaccc ccgaactcca acccccggga ggcagaatat tagcttgtcg
     5701 ggtagcatcg acaaattgca tattaccttg gttgagatac agtttatcct gccctaaatt
     5761 gccggtaaaa aacaaatcgc aatagccatt cccgtccaca tcgcctgctg caacgccacc
     5821 gccgttgtag aggtattgat acagcagcac attcatggta gctgtttcct gtaaatcgag
     5881 gtgcatggca acaccggttt gggaggcgga aagtcttgag aatagggtgg tttgggcaaa
     5941 tgtaggtgag taagcaacaa gtgcggaaac aatccaatat gccacaaatt ttctattcat
     6001 gcagaaatac cctgttttag gtagtcaaaa ttatgtatta attaattgta agtgcggttt
     6061 gttgaatatc ttattaaact aaagtaatag cgctcgaaaa ccttacaaaa aaattctaat
     6121 tgcggtaatg attgagtaaa attgcattga ttttcgcggt actatcaccg ccttgatcaa
     6181 aatgacctat cagtattatt tcttaaacca aaaattttat tgttcatgat gcatgtttat
     6241 gctctcatca gacgcgccat tttgcccgtg gtgttgttga tgccggttct tatttgggga
     6301 caggcgccaa cattgacagg ggtgatctcg gatcagtcag gtgctacgct accgggggtt
     6361 acagtgctta ttagcggcac cggtaaaggc acaatttccg acttcgacgg atcttatgtg
     6421 cttgccttcg acgcagcggg aacctatact gtaaactata cctatatcgg gtataaagca
     6481 gtgaccgaaa ccattacact caccgacggg caaaacctga aaaaggacct cattatgcag
     6541 gaagacgccc ttatgctcga aacagcggtt gttgtgggtt atggtactat caaatcaaaa
     6601 gaccttacgg gttctatcac gaatgtaggg gtgaaagact ttcagggagg taacgtaacc
     6661 actccggagc agctgatcac aggaaaggtt gccggtgtgc aaattacctc taactcgggt
     6721 atgccgggtt cgggaagtcg catccgcatc cgcggcggta cctcgctcaa tgcaagtaac
     6781 gatccgctca tcgtaatcga tggtgtgccg gttgacaata acggtatcag cggctcagga
     6841 aatgcgctca gcctgatcaa cccggatgat attgaaaata tcaccgtgct taaagacgca
     6901 tctgcagccg ccatctacgg ttcacgcgcc gccaacggtg taattatcat cactaccaaa
     6961 aaaggattct ccgataataa attgcacatc gatgtaagca gcacatcatc actatcgcaa
     7021 gtgaccggtt atgttgatgt gcttactgca gatcagcttg ccgccgtgct cgatacggca
     7081 ggtactgcta cacaacaagc gcttttaggt tcagagaata ccaattggca aaaagaactt
     7141 taccgcatgg gttttgccga agatcttgac ctcactttca caggtggtat caaaaatctt
     7201 ccttatcgcc tcaacctcga atacatgaat gaaagcggtg tgctcgatcg ttcgcaactc
     7261 ggacgcatgg gcgcgtctct cggaattaat ccaacattgc tgaacggaaa attaaaaacc
     7321 gaagcaaatg gtaaatatta tcataccgat aacttcttcg ccgatcaggg tgcaataggt
     7381 tctgcaatta cattcgatcc gtcacaagct atttatgacg atgcttcggt ttacggcggt
     7441 tattgggaat ggacaaatac atcaggtgga ttaattccgc tcgcgccaaa aaatcctgtt
     7501 ggattattaa atcagcgcga tgatgaaagt aatgtaaatc gctttatcgg aaacattaaa
     7561 ctcgactatg aaatatttaa agattttcat ggagtaataa atggcggtat cgatatggcg
     7621 cgttctaacg gaacagttgt tgttcctgcc gaagcagctt cacaatatcc taacggttca
     7681 aataatcaat acgaacaaag caaagacaac aaattgctcg aagcgtatgg taactataaa
     7741 aaggatatta aatcgattaa aagccgcatc gattttacag caggttattc ttatcaaaac
     7801 tggcttacgc aatcaccgtc atttccaagt ttaagtttcg ccggcgatac tatcactcct
     7861 gttggtatcg acttcgaaac cgaaaataca ctgatctctt tctatggtcg tttaaattac
     7921 aatctgaaag agcgctacat ggttacggct acattgcgcg aagatggttc ttcacgcttt
     7981 agtcccgaca accgctgggg tttattccca tcagttgcag ctgcatggct cgtaagtgat
     8041 gaagcgttta tgtcgaatac aaaaatgtat ttgaaattac gcgcaggtta cggagttacc
     8101 ggtcagcagg atattttcta tgattatccg tatatcgcaa actacgatag ttcaacctca
     8161 acggctcaat accaatttgg tgatgctttc tattatttac ttcgtcccga tggttacgat
     8221 ccgaatatca agtgggaaga aactaccagc tttaatattg gatttgattt cggcttgaag
     8281 ggagataaaa tttcaggtag tgttgatttg tataaaaaac tcaccaacga tctccttgca
     8341 accattccga ttccggcagg caccaacttc accaaccaaa ttcttactaa cgtaggaagc
     8401 atggaaaata ccggtgtcga aatcaacctg agttatatcg ctctcgacaa caaagacatg
     8461 tcgttagaat tcggaattaa cggaaccgct aaccgcaatg aaattacaaa ccttactttg
     8521 gtagaagata cttcaagcgc cggcatttta gttggtggta tagctggtgg tattggtaat
     8581 actattcaga ttcaatctgt tggttatgca tcaaatactt tctttgccta tcaacaacaa
     8641 tatgatgaaa atggcaaccc tatcgaaggg tcatatgttg atcagaatgg cgatagcatc
     8701 atcaatgcag atgatctgat ttggggtgaa aatccaacac ctgactttta tctcggtttt
     8761 agcgctgcat tcaaatacaa aaaatggagt gtatcaactg tgttgcgcgg tgaattcgga
     8821 aaatacatgt ataataacat caattctaca cgcggtattt tccaaaacgt tccaagtgca
     8881 agttatatgc aaaacctgtc gaccaattat ctggaaacgg gtttccaatc atatcaattg
     8941 ctgagtgatt actatattga gaatgcatca tttgttcgta tggacaactt tgccgttgga
     9001 tatgacgttg gacgagtatt caatgacaat gcgtctctca cagtgggtgc agttattcaa
     9061 aatgtatttg taatttctcc atattcagga cttgatccgg agattgcagg tggtattgat
     9121 aattcaattt atcctcgtcc acgcatttat tctctcaaca ttaatcttaa actctaaacc
     9181 gaaaagcgat gaaaaataaa atattaattg cagtcaccgc actcgccatt tccggtttta
     9241 cgttgagctc atgtttcaac gaccttgacc tggcgccgcc aagtggcatc aatgccgaaa
     9301 cggtgtacaa caatccggat aattatgtcc atgtattggc taaattatac gcagggcttt
     9361 ccatttccgg caaccagggg cctgcgggta atcctgatat cgccggtatc gatgaaggtt
     9421 tctcgcaata cattcgcgta ttgtggaacc tgcaagaggc aacaactgat gccgcaaaat
     9481 gcgcgtggaa cgacccgggt attcctgaat taaataaaat gcagtggtca agtaccaact
     9541 catttgtaaa tgcgatgtat tatcgtattt atttccaaat tccgttgtgt aatgaattta
     9601 tccgcgaatc ggcagatgca aaaatgtctg agcgcggatt ttctgatgca gagcaaacta
     9661 ctattcgcgg ttatcgtacc gaagcgcgtt tcctccgtgc attgagttat tatcacgcat
     9721 tggacctttt tggaaatgtt ccattcatta ctgaagacga tgttgttggt gctttctatc
     9781 ccgaaaggat aacacgtgcc gagctattta attatgttga gtctgaatta ttagcagtcg
     9841 ctaccgaact tcctgcacca cgtgcaaatg aatatggtcg cgccgatcgc gctgctgctt
     9901 ggactttgct ggctcgtttg tatctcaatg ccgaggtata taccggcact gctcgctata
     9961 ccgattgtgc aacatattgt cagaaagtga taaccgaagg tggttattct cttgaacctg
    10021 tttatgatca tttgtttatg tcagataatc aaggttcaaa cgaggtaatt ttccctgtaa
    10081 ctttcgatgg tcagtttaca caaacttatg gtggaacaac attcctgaca cattcaactg
    10141 ttggtggtga ttctttgagc caaattgata aagcatatta tggtattgaa tttggttggg
    10201 gaggaaatcg ctcaacgcca gaatacgcat ctttcttccc tgatgcaaat gatgcgcgta
    10261 atatgttcaa aacatttgga caaacactcg agtttacaga agatgcacag cttggacaat
    10321 ttgttgttgg ctatccgata gcaaaatggc gcaatgtatt gtcaacttca gttgccgaat
    10381 tcactgctac tatttcgggc gatgtaatga ccgttacatc tgttgccgat ggctccgtgc
    10441 aggtgaatca aaacctggca ggaagtggta ttgccggtga aaataagatc atcgaattta
    10501 tcacaggaac aggcggaaca ggaacctatc gactggccaa cgctgataac gttacatctc
    10561 ccaccaccat tcacgcggca attccgggaa caaacctcga tgccggtggc aactacaccg
    10621 atattgattt tccaatgatg cgccttgccg atgtttatct gatgtacgcc gaatgtgccg
    10681 ctcgcggtgc aggtgatgcc ggattaggtg tagaatacgt aaatatgctt cgcgaacgcg
    10741 cttatggcga tgttgcacat aatgtaggct caattactcc cgagtctgtg ttggaagaac
    10801 gcgctcgtga gttgcaatgg gaaggatatc gcagaacaga ccttattcgt tacggactgt
    10861 tcaccggcgg atcttacctc tgggcgttta aaggcggtcc attcaacggc acttcagttc
    10921 ctgaaacact gaacttgttc ccgattcctg ctgcagatgt tgtggcaaat cctaacctcg
    10981 ttcaaaatcc gggatattga tatattaaat attctttcaa aagcctccgc attgtcgggg
    11041 gcttttttgt cctattgcaa ttgctcttaa ttacaaaaac ctttcctgag gggaagtgtt
    11101 atcctcatat tcaactattt ttactgcatg aagatcaaaa acctcctgct aatttgcttt
    11161 ctggcttgca caagtgtcat atcagcacag caaattgatc agatctgtcc gccaaactgg
    11221 ttcatcggta tgcgcgattc cagtgtgcaa ctactaattc atggaacagg aatcggaaat
    11281 tatgatctca aaatcaatta tccgggtgtt cgcctcgttg ctgtaacacg tgtagaaaat
    11341 aaaaattact tgtttgcaga tcttgttata gataaaaccg ctcgccccgg ggcagtagac
    11401 ctttattttt cagcaggaaa aaaatcattc accaaacaat gggcactgca agcaagggcc
    11461 ccttggacaa atacatatgg actcgatgcc ggtgatctta tttatttatt aatgcccgat
    11521 cgctttgcaa atggtgacac cacaaatgat gttgttgcaa gcatgaacga aacaataagc
    11581 gatcgcaagg atccaaaagg aagacatgga ggcgatattg ccggtatcat gcaacacctg
    11641 gattatatca gcaagctcgg agcaactgca gtttggtgca cgccgttggt tgaaaacaat
    11701 gaaccaaaat ggagttatca tggatacgcc attaccgatc attataaaat agatccgcga
    11761 tttggcaata atcaacaata cgctgaattc gttcaatcgg cacacaaaca caatctgaaa
    11821 gtagttgttg atctggtgca caatcatgta ggagatcaac attggtttat taaagatctc
    11881 cctatgccgg attggattca tcaatgggat acaagttttg ttagaagtaa ttatcgcaca
    11941 agtgtaaaaa acgatcctta tgccagcgcg tatgatgtaa aaaaaatgaa tgagggatgg
    12001 tttgataaac atatgcctga tttaaatcag gaaaatcctt atctggcaaa atatattatt
    12061 caaaataata tctggtggat agaaacatat catgttgatg gatttaggtt agacacctat
    12121 ccgtattccg atctttcttt cttacaggat tggggcgttg ccatacaaaa agaatatccg
    12181 ggattnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggaaa agaaaaggag
    12301 atatttaatt atgtaagcgg attagctaac tggcgcaaaa attctgacgc cataaaatat
    12361 ggcaaactca tgcagtttat tcccgaaaat ggcgtgtatg tgtatttccg atatactgaa
    12421 aaagaaacaa taatgattgt actcaatagt gcatctacag aatacacact cgacacaaaa
    12481 cggtttgcag aaagaatcgg caatttcacg attgcacagg atgttgtaac caaacaaaat
    12541 aatactatcg caggactaaa agtggcggct aaaagcccgg gggtttatgt gctgaaataa
    12601 aatattaagg tcgattctaa aatcactgta atttaagtct caattaagca cgagttttga
    12661 ggattttatt tctgcaccgc gatcagaaat aaatctgacg atatatattc cgcgcgccca
    12721 gtctttgcaa tcaattacat tattcgctcc ggaagctaca ttgcgaccgt caagcgacac
    12781 aacgtcccaa cttgcgttaa caggcaggtt tgaaatgact gcattttcat ttgcaggatt
    12841 aggaaatatt atcggtgatt catccggttg aatagtcgta tttatatcaa tggtataatc
    12901 aatttcccat ccaatttctt ccaataatgc cagtgtcata ggacccggaa cctggctgga
    12961 atgtcctgat gccgcattgg gtgtcatgaa ttcattttca ttgccaacag gatatgaacc
    13021 ttcgttccaa tgcgacatgc tgctgccaag cgtaaatgtt gaaggtgcat aaattcttcc
    13081 cgcttcacca ccatgcgcat tcaacacttt ttcacttata caaaaaatat tgttactagt
    13141 aaattcatca cccagggcat ctgatggatt ttcaaatgtg agcaacatcg tacctgaacc
    13201 atttgataaa taatgatcca ttgccgaagg aagcgtatct aattccggcc atggaaatga
    13261 tgtcaccaag ggaaagaagt ccgttgcata tataagtccg aatgaccctg tagttacatc
    13321 tttattgctc aaacttaaaa atcccaaacc atgacaaatt tcatgcatca ctgtcgacac
    13381 aaaatcatat tgccccgcgg gagtattgcc gtcggttcca aaataccaat ttgctgtact
    13441 gttgatgaaa atatccatat cggcatatcc cggatttaat tccgttcctg ttattgcatt
    13501 cgccaggcaa cttgcatacc aggtatcatt aaatggtgcg ccaacaaaat ttttctcacc
    13561 attaggaaaa gtaatgccca attgtccggg gagcagtgtc actaaattgg cgtgcacttt
    13621 tatcggcaca tcactaatca ggtattgact ccattggtct gttgcgtagt taaatgcggc
    13681 ttcggcttct gcatcaaatc cacctaaagt gacttcaaaa gtggcatttt gtgactgagc
    13741 aaaaaatgct gaaaaattta ttccgataat aaggcaaagg gtgcggtagt taatcatatc
    13801 aaggtgttgt gacctttcaa aactaatcag ataaacgcgt attttcctta catcgcatcg
    13861 aaatggtgac atccggcatc tgatttcacg ggggttttcg gtaattttat ggccgtaggc
    13921 atgaattttg gattccaata tgcgcgcgct ttggtggtga tgggtttttg ccttatcaca
    13981 agttctcttt ttgctcaaaa cagaaccatc aacgggaatg tgcgcgataa gaaaacaaat
    14041 gagtctgtgc tctttgccgg aatttatcta cttcacacag aaacagccgc acaaactgat
    14101 gaaaaaggca atttctcgct gatcgcgcct tccggatatg acactattat tgtaacaagc
    14161 ataggatata tcacagatac cattgtactt ggacgcgaat tgacgcagca attgaatgtg
    14221 gcgctcgacc aatcgcttta tgaattaaat accatggtga ttaccgcgca ggaagaacct
    14281 gcaaaaacaa ttatgcgcaa ggtgattgaa aacaaaaaaa taaacgatca gcaacatctg
    14341 gcagcatata aaacacgcgg gtataacaag atagaaattg acctcggcaa tctcgagatg
    14401 cttaccgatt atgattccat tccggttgac acaactaaaa aagaaaaaaa accaaatcca
    14461 tacgcggtcc tgctcgatca tatcgatagc acaagcgacg tgacgcattt cctgccattc
    14521 ttctttacgg aaactgtttc agatttctat tacaggaaaa atccgaaaac cagaaaagaa
    14581 ataattattg ccagtcgcac ttcgggtatc aataatgcca gcgccacaca gatcctggga
    14641 aattattatc agcagtttaa tctatatgat aattattggt tcttgctaag taagaatttt
    14701 gttccgccta tcaccgacgc atggaatttc ttctatcggg ttgatcttgc cgattctgcg
    14761 tggatagata acgactggtg ttatctgatt cgctttcaac ctcgtcaaca gcaggaaaat
    14821 acttttcatg gctttatgtg gattgccgac agttcttttg ccgtgaaaga aatttccatg
    14881 cagcttgaca gtgccgtaaa tatcaattat tgcaagcgtg cacagtttta tatgctattc
    14941 gatcattata atgattcggc atgggtgttg aaggaagaca gactggtggt agagtttctt
    15001 ccaataaaaa attcaacaag ttttatcggc cgacgaacaa cctattatga tgattacaat
    15061 ttcgatccaa atgtatttga ggaggttgtt aaatataaag acgatattgt atataccgat
    15121 agcgttgtaa ataatgatga agcttattgg cgtagtgttc gtcctgattc cctgacatac
    15181 aatgaaaccg gcgtatatga attggtagat agcctgaaga acattaaatc gtttatgacc
    15241 gttgttgatg tgtataatac tttaatgtac ggatattgga atctcggcta tgttagaatt
    15301 gggccatttg ccaacatggc gagcagtaat gatgtagagg gatttagagt gcgtattgga
    15361 ggaaagaccg gcgaccttat cagcaaaagg ctgactattg gcggttacgg tgcttatgga
    15421 tttcaggata gaacatttaa gtatggtgct gaatttcacg ccgttattgc caaaaagccc
    15481 tggcaggaat ttgatttaaa atattatgat gatctggatg taagcaacaa tgataatgta
    15541 accttcggcg aggataacct gctatccggt ttttatcgtc gtcgcgatac gccacaaaaa
    15601 atcgttgata ttgaaaaatt tcaggtgagt tataaaaaag actggatttg gggactctca
    15661 aatacaatta cactcaccag cacacacatg catccgttgt tcgacattta ttattatgat
    15721 aacgattcac ttcgaagcaa tatcacaaac tcagaaatta aatttggttt gcgatttgcc
    15781 tatcgtgaaa aatttatgtt cgataattat cgcaggtata gcctgggaac aaaatatccg
    15841 aaacttgagc tctattaccg cattggtata cccgatttaa tggaaagtga tttcagatat
    15901 actgcttttg aagtaaatat ctgggatttc tttacggttg gaagtatcgg cggaaccagc
    15961 tatacgctca aagcgggcaa aatattcggc accttgccaa cattattgtt ggaaacgcct
    16021 ccgggcaatg aaacatattt tatgaatcat gccaatttca acctgatgaa tgagtatgaa
    16081 tttgtaaacg atacgtatgc cgaattattc cttacgcata gtttctacgg tttcttcctc
    16141 aataaaatcc cttatgtaaa taaactaaaa ttgcgtgaag tggcaacttt taaactcgca
    16201 tacggttctc tgagcgatgc aaatgttgag ctaaatgatt atttaaaaaa tagtgcccca
    16261 tattatatag gcaacacttc aatgaccaat ctcccatata tggaagcggg cattggcgta
    16321 gagaatattt ttaaactcat tcgcgtagat gccatctggc gactcacata ccgcaataat
    16381 ccacttgcac cgaatttcgg cgtacgactt ggtgttaatc tggatatcta agaggccact
    16441 ataaaaaaca tgatactttt tgtctagcgg tttctatgtt taaagtttac tgattttcac
    16501 aacccctgca ttatcgccgt aaaccaacct gcagaaataa ataccggctg gtgcattgct
    16561 attccatgta aagctaaata ttccttcagc ctgtttacca aggtcatacc tttcgatcat
    16621 tgatccggtg ttgttataaa attcaagcac aacatcatcg gccgtttgca gtgtagtcaa
    16681 tatttcagtt tctccttcag ttggatttgg catagcaata attgaattgt tggccatttc
    16741 atcaacatga tttatgcctt caataatgtc aggcgtcgac aagcgcacat cggtatacaa
    16801 tcggtattct cccggcgcca tggtaaatga agcaagtaca tcgctaacgt ttaaggaatc
    16861 tccgctgaaa tattcatacc accatcctgc atgttgaaag ttgggtgaaa tatttgccag
    16921 gttcacatca aaattcccta acacggtaac attcatatcg ggatcattca aattaatgcg
    16981 ctttccgaaa cccgcaacac tcatattgta gtttgtagtg ctgaatgccg gatatgttgt
    17041 tttcaactta attaattcac tccacacctg gtaaatgcgc aggcgatttg cgttggaata
    17101 gtaatcccaa cgaataggtt tattacatac gcgacaacca tattcaatac tatagtcata
    17161 tcctaactca ccgaattgcc acaacatttt cggtccggga attgtaaata aaaatgcacc
    17221 ggctaattct tctcttgaaa gcgcaatatt taaatttttt acattgtagt ccggattagt
    17281 ggtattacca taagtaaggt ttttatacat caaccgctct tcgtcgtgac tttccatata
    17341 tgttaccaga tgcggatcat cccatccgcg cgattgatag cttgcccagt tcaaatcaga
    17401 tccgccataa cccatggtgc cttgattgta gttgtaattc atatttcccc acaacataaa
    17461 tcctgcgttg ctgagtgttt cttcttcgct gttatcggca aaatgttcca gaatcaattt
    17521 agcatccgga ttaacagccc aaattacgct gccaatgttt tcccaaatag caatgcgtga
    17581 tgcatcatat gctccccatg cggcaacgtt gcccaatgta tttgtttgcg tatatccttt
    17641 tgaaagatcg aagcgatagc catctacatg atattctgtt atccaatgtg tgaatacaat
    17701 atttctaaac gcctctgtgg catcactttc gtgattaaaa tcatatccaa cgttataatc
    17761 atgcgttggc acctgattaa aatatggaga atttaatgcc ggtgcgtttg ctgttgcgtc
    17821 ccaccacatt tgcactaacg gacattgtcc gaaagcatgg ttcatggcaa tatccaaaat
    17881 tacggcaata ccattggcat gacaactgtc aataaatatt ttaagtgcct cttttgtgcc
    17941 gtaatattta tcaggtgcaa tgaagaaact tggattatat ccccagctct cattgccttc
    18001 aaattccata atcggcatca attcaatagc atttacacca agtgttttta aatatgagat
    18061 cgtatctgtg agagttgcat aattatgtgc ggcaataaaa tcgcggatta acaattcata
    18121 aataaccaat ttttctgttg gcggtgaggt aaaatccgga accgcccaca catagtcggg
    18181 ttgtgctgtc tgaaataccg aaacgatgcc actggctttt cctgtcgggt aatcaataag
    18241 tccgggatat gttgcttcat tgatataacc atcgctccaa ggatccaata ctttttctgc
    18301 tagcggatca gcgacggtta aattgccatc aataaaatat tgataaggat attcttttcc
    18361 cggttcaagt ccgcttaatg tgatccaata agtggcacca tcgggatcgc gcttcataaa
    18421 tgtttcgtcg gtcagcaacc aatcgctatg ttctccgatg gcaaaaacat aatttttata
    18481 tggcgcatat aacactaaag taacggtatt gtcatcaata taatttattc cctgatgcat
    18541 acctgccgga accgcttctg tagttactgt tcctaaaaca taaaaataag tagtgtcgta
    18601 cataacatcg gcaccggaaa ccgccttcac cacaatatcg gataatcccg ggtcaaccgc
    18661 agttaaatcg tatgtaatat tgctgtcttc acccgcaatt atttcaacgc cattaatata
    18721 gagatataaa ctatcgctgt aagtgctgct tgcttcaata tgtacattgt cgcccggatt
    18781 taccagatat ggattataat caggcaccgt aatagcaaca ttcaaaccgg gtgcatatac
    18841 atttacaaaa atatcggagc catcaacatc tctgccgaca attgttcctg cagcatttcg
    18901 aaaaacaaat gccatttgat aaattgtatc gtctgcggcg gcgccataaa aatccatgat
    18961 gcttgaaaat gtgatggaat atttatcatc acccaaactc gtcatcaaca cttccggatc
    19021 tgcagttccc caaacacctt gaacgtattg ccagtcgctt ggcgtatcgc tgtcctgggt
    19081 gataagacct gtatgcgcat aaacatcacc ggtataaccg gcaagtgcgg cattaccttg
    19141 cgatgcgtcg aaaattacag ttaccggtcc atcaggagtt ggaaaaattg gatccgtgga
    19201 aatgagctgc gctgaaagcg tgaacgccat taacgtcaca atgctgcaaa gagtaagcct
    19261 taaattcata cagtaaagtt actgtgcaaa ggttataacc tcaaccgcag catgaaaatt
    19321 attgcctgca aaatgtagag acaaatgtta aacaaaccga cttatatata atcactttca
    19381 aaaatattcg ccgttttgcg actcgtcaat ttacaatcaa taacgaaatc aatataattt
    19441 gcgaaaaatg caaatgttcg ccacattcga agcgcatatt tgcgaaaaat gcaactaacg
    19501 acagctatgc agattcgcgc tgcaatatcc ttttgtccaa aataaatggt ccaaagggaa
    19561 gtatcgaagc cagaacggcg agcgcggctt taccaaaagt ccattcgtgt tctattttca
    19621 cctgaagcaa tgtgaacata tagctaatga acaacaagcc atgcatccaa ccggcatatt
    19681 ccaccgcttc gggtatgttg gcagcatatt ttaatggcat cgcaataccc agtaaaagca
    19741 ggaatgaaat cccctccaat atcgccacca ccctaaaccg ccccaacgcc gaagcatacc
    19801 atttcttctc catacaccta aattttgaac aaaactacca aaccccctcc tctttcctct
    19861 ctcctctctc ctctctcctc tctcctttct cctctctcct ttc
//
LOCUS       DAOQHY010000046        19518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1261_length_19518_cov_5.133895, whole genome shotgun sequence.
ACCESSION   DAOQHY010000046 DAOQHY010000000
VERSION     DAOQHY010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 19518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19518
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1261_length_19518_cov_5.133895"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2056
                     /locus_tag="PLL28_05510"
     CDS             <1..2056
                     /locus_tag="PLL28_05510"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNF68812.1"
                     /translation="ELWKKDTVMYANILAYTEGINAFIDPLLEEGYEAYPIEFKLLDY
                     KPEEWTPLNSALLLQYMSSTLTGFDDDLEYSNLRKLLTPEEFAALFPDRPEGIDPIIP
                     SEKVYNFTVNDADSIPQDARTGYFKKVLEIFESQHDHTQIGSNNWAVDSSRSKSGHPI
                     LANDPHLQLNLPSLWFEIQLQTPATNCYGVSLPGAPAIVIGFNENISWGVTNAGIDVR
                     DWYTIEYKDDTKSAYKYGKTWKKTEQRIETIDVRNADPIIDTVIYTHYGPIVYEDEEI
                     TAGTKQDPHKVQRIDLAMKWAALEPGEQIKTFFYLNRAKNYNDYRQAIAYFNCPAQNF
                     VFASHNGDIAITQQGKFAVKKKEQGRFVLNGSDPGDDYTDFIPFDRNPGILNPMRGFC
                     SSANQHPTDATYPYYYTGFDFEFYRNRIINRSLDAMHDATIESMEKLQQNNYNLHASE
                     ALPLMLDKLTYDVGTPDSTAYTVKTALSEWNFMNDADMLAPTYFQMWWDTLYAMMWDE
                     MEDSVIAYVRPNKYNTVKAMHAFPNDQRLFDMISTPDQKETLDELINLSYGIMVHMVD
                     SLAAEDKNLLQWYRHKRTNIMHISQIPAFSKMSVHNGGYQSIVNATSETHGPSWRMIV
                     EMSNPVKAVGVYPGGQSGNPGSKYYDNFVDDWAAGRYYELHLYQSEEEAKEQAQFTMR
                     FN"
     gene            2058..2402
                     /locus_tag="PLL28_05515"
     CDS             2058..2402
                     /locus_tag="PLL28_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68813.1"
                     /translation="MKYFVSITIGFTLCMAGQFFLPWWWIFAPLTFLISFIVNYHSAW
                     RAFFAGLLIVFISWMAAYIYKDLANDSIMSGKMANLFNFKNNYALFFIVAIAMGIIGG
                     LTSTAAYFTRNS"
     gene            2406..3308
                     /locus_tag="PLL28_05520"
     CDS             2406..3308
                     /locus_tag="PLL28_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68814.1"
                     /translation="MSDFDVLLITEDRYESPQHIDGYIANILQDDAILTAAFEEVGLR
                     TKRVSWSNPEIDWSASRCGLFRTTWDYFLRFNQFKNWMEQTAQKVIFINPIKVIRWNM
                     DKHYLADLKATGIRVVDTTFIEQNTSCDLVALLQASGADSGIVKPAVSGTARHTYVVD
                     NSSLESVQPIVNDLLKSEALLFQPFQKDILLNGELSLIIIDGKCTHAVRKTAKQGDFR
                     VQDDFGGTVHDHIPTPEEIQFAESAVAACPEKPLYARVDIVRNNAGDLAIMELELIEP
                     ELWFRKNPAAAKPLAAAIKNIYFS"
     gene            3305..5248
                     /locus_tag="PLL28_05525"
     CDS             3305..5248
                     /locus_tag="PLL28_05525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68815.1"
                     /translation="MNKLFITSCLILIANISMHAQVSSDYAVLLSAETSVAPPTITLN
                     WPAYADASQYEVFRKAADDVSWGTPIAILAGDATAYDDTDVIIDSMYEYKVKRTNISG
                     LEGEGYLLGAITLHAVDYRGTCLLVMDTTVTDALTNEITRLMDDISGDGWSVSQLHVA
                     RDMSPIAVREAIIAAAEADPEMQSVILIGRVPVLYSGNLYPDGHPDHQGAWPADGIYG
                     DINADYTDVTINTTTAGRPENWNTPGDGKYDQSIFKSKIELAVGRIDMYNMPSFALDE
                     TGLLKRYLDNDHKFRTGITHFEQRALIDDNFGAFAGEAFASSGWRSYAPLVGNDNLQE
                     LDYFSTMYDNSYICSYGCGGGWFQGAGGVGSTTDFTLDTVKTVFTFLFGSYFGDWDVN
                     DSFLRAPLAGGYALTNAWAGRPHWSLHYMGLGYPIGYASRQTQNNQSTYTSNIFPKWV
                     HIALMGDPTLRMQVIAPVSEMHCEISDIQNAIDIHFTTSADPEVIGYYVYRAATTYGK
                     YERISDEIVATSPYTDLTPIDGLNYYQVRPVMLTTTPSGTFYNTGIGLRDSISIDVTD
                     VNTLAPSIVHLYPSPADTYIVVVTSSDLVGSTFIIYDISGKAVLTVKLLSEDTSIKTT
                     TLPDGVYTLKFNNGITRQFVVSH"
     gene            complement(5295..6173)
                     /locus_tag="PLL28_05530"
     CDS             complement(5295..6173)
                     /locus_tag="PLL28_05530"
                     /inference="COORDINATES: protein motif:HMM:NF014708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNF68816.1"
                     /translation="MMKLRTVRALQYADMMDMGGMPVRQPLPSAAIDQVDPFLLLHHH
                     SSKIAAGAYPREVGVGPHPHRGFSPVTFIFKGDVHHRDSRGNSAIVHAGGVQWMDAGM
                     GIMHSERPSKELAEKGGSQEIVQLWVNTPAARKMDQPNYQALQENDIPSISIDDVGSN
                     IHVVAGTFEGVKGPAHSKLDLLMLNVTMKAGIKRQFEIPTDHQTLLYVVKGGLTMTGY
                     GPVPALHMVVLHQDGAGIELTADQETIFLILSGKPIDEPVIQYGPFVMNSQTEILEAM
                     RDYQMGKMGILIEEFD"
     gene            6295..7167
                     /locus_tag="PLL28_05535"
     CDS             6295..7167
                     /locus_tag="PLL28_05535"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNF68817.1"
                     /translation="MQAIGFYLLLPFLYFFSVLPMFILRQLSNVAFFIMYYITGYRRE
                     VVMRNLGHAFPEKSEQDLKKLARGFYLYLSDTLVETIKSFTISDAGLRKYCAINNYDV
                     VQAEIDKGRNVMFVLGHYGNWEWGGGALAKRAPIPLKAAYKPLSNPYFEKLMFHTRTR
                     LNIRLIAKRKILAHLKDHADEQTLLVMIADQSPNPKEHFWVKFLNQDTAVVTGMEKIA
                     RQYNYTVIYSTIASVGRNKYEIRFKKITDDPQSLAPEALTAMFMHELEQDIYKNPAPY
                     LWSHRRWKLKKPQA"
     gene            complement(7164..7784)
                     /locus_tag="PLL28_05540"
     CDS             complement(7164..7784)
                     /locus_tag="PLL28_05540"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNF68818.1"
                     /translation="MKRIMLILFCVAAVDLQAQEYELGVKGGVNYAQSVILNVVSLDG
                     VDVADVESQKGSALVFGGFARATFGRWIVQPEILFSENQSLVTLADANVNEMDLSDLF
                     SMSVDKCDVPILLGYQAFGSVKLMAGPVFTTVRSTMSDPLFSLKDMTVGYQTGINFDI
                     NKLTFDARYEGNLSKFKEYVETEKGMLEVDTRKSLFQFTVGYTLFD"
     gene            7889..8632
                     /locus_tag="PLL28_05545"
     CDS             7889..8632
                     /locus_tag="PLL28_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNF68819.1"
                     /translation="MSATPEWNIRLEPRNIKYLSGPRSRWRELWWAFSVFTEFIKGFR
                     ALHFIGPCITVFGSARFKPDHQYYKQAYDIGKKMSALGLTVLTGGGPGIMEAANKGAF
                     ENQGRSVGCNIVLPQEQKPNPYMHKWVTIKYFFVRKVLLVKYSYGFVVMPGGVGTMDE
                     FFETLTLIQTATIHNFPVVMIGKDYYKDIWEMLQKMTQAGTVSPEDLNLVLFTDNAQE
                     AVNHIDFYIKKHYSFKKVKPRKLLLERFV"
     gene            8651..8785
                     /locus_tag="PLL28_05550"
     CDS             8651..8785
                     /locus_tag="PLL28_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68820.1"
                     /translation="MRIPEKTMMWLKRFGWAGFFFFLIKGIVWLIAGAAIIEWFQKTF
                     "
     gene            8887..11268
                     /locus_tag="PLL28_05555"
     CDS             8887..11268
                     /locus_tag="PLL28_05555"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF68821.1"
                     /translation="MKQITLFLFCLTIGTFARAQQLTQTIRGQVTDNQSKATLPGVNI
                     MVLDSDPILGTTSDLDGYFAIEQVPIGRVSIQATFIGYEPFIVNNLELTSGKELMLNF
                     SMSEQVITMNEVVVRAKDDKMETNNEMTTVSARQFTIEESMRYAGARNDVSRMAQNFA
                     GVRGSNDAVNDIVIRGNSPVGVLWRLEGIDIPNPNHFGDLGSTGGPVSMLNNNILANS
                     DFLTGAFPAEYGNGISGVFDLKMRNGNYKKHEFLGQVGLNGFEFGAEGPLSKDSKSSY
                     LMNYRYSTLGLMSAIGINFGTGTAIPYYQDITFRVNMPTRKAGTFALFGLGGVSSIDL
                     ISSSTEDSVDNLYNNDLDIYDKSKIGMVGFTHQYLINDKSYTRFTLSTSSIENTDIID
                     SISSENFEPINYYNQNFTQHKASASLMYKIKLNSRQNLQAGASADMLFADLNDSIYRN
                     TLDRWITITSYNGNTMLLQGYVQYQIRLTENLTATAGVHDQFLALNGSNSVEPRAGMK
                     YTINEKNAISFGYGLHSMMAPISVYFKTVETTPGNYITPNDSIDFIKAHHFVLGYDRQ
                     LSSTMRFRSEIYYQHLYDVLIDRDSSSYSVLNTGSMTFGSPDYLVNKGTGRNYGAEVT
                     LEKFLDKGLYFLVTGSIYKSEYLGSDDIERETAFSGNYVANVVGGKEFAILAGRAGAK
                     TKKSVVFDTKLTMAGGQRYTPIDLTASIDEGKTVYDWDKAFEAQFDDYFRWDVRLAFK
                     MDGKHASQEWAIDVQNVTNEQNPLYQRYNPVSQTVNTIYQLGIFPVPQYRITF"
     gene            11265..12326
                     /locus_tag="PLL28_05560"
     CDS             11265..12326
                     /locus_tag="PLL28_05560"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNF68822.1"
                     /translation="MSKQSYIPFRKALAGTLVVSLVPLIYQILVYYIVGTPKCPELTF
                     WENAMWTAVFSLLVTFALFIVNIYINTKGHELLEEKVNDGIRIFVTVFVSLLASNIVI
                     FVMWKLFNDLILHLPEDGETARVFSNQILATVLVIIVDLIIEVDIYVKKLKISMETQA
                     RLEKEFIRAQLESLKTQISPHFLFNSFNALQSLIDQSPVQAKSFIQELAKVYRYVLDK
                     KDEMVVPVSDEVAFIRSFLYLNHIRFGDSLSADIDLSAQEMDGFIPPLTLQLLVENAI
                     KHNIISQARPLHISVFRDAGNIIIKNNLQLREDTPSASGIGQSNLVARYNLISDRTPS
                     FNIVDDAYIAKVPVLEQEL"
     gene            12331..13077
                     /locus_tag="PLL28_05565"
     CDS             12331..13077
                     /locus_tag="PLL28_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNF68823.1"
                     /translation="MHVLIIEDESLAANWLRTQIEQSTKDMTVVAVLDSVSASVDWLQ
                     KNQVDLIFSDIHLADGISFSIFERIAISTPIIFTTAYDHYAIRAFQVNSIDYLLKPID
                     TEALSASIEKYRSLHTNIQPDWKMLVSGLQPKNNYQERFLVSTGQKLKSLPIIDIAYF
                     FADNKLNFVCDTNGRTYILDQTLDKLEQVVDPGTFFRINRQFIVSFGAIQDMLHYSKS
                     RVKLTLHPSTEHDTIVSAERSAAFKAWLNR"
     gene            13126..15399
                     /locus_tag="PLL28_05570"
     CDS             13126..15399
                     /locus_tag="PLL28_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68824.1"
                     /translation="MKYWMLLSAIALASCSATLENEPITHTNWPTREELFQNFRNQDT
                     AIIVYGKEDYKISDLLVNGVAALAPKDDRFTVITRRDDKLTEFEAANYPLYIIGTRNN
                     LLIKRVSEAIPVKVFEGGFNFNGTDYMAPNDVIKLSFYPNVFNPKIPFTVITGNHEEA
                     IVQYVQQEMNGEWGYFFWDNWGYQVYHNGRRTILGNFSQDSASLWSIDKKQHWEFDFE
                     GKSLKTSGQVHYTGHNDPNVTNLDNWDAVAQDDLTRISEFCGKKISARLEVHLYPTPE
                     TKTLLMSDPDQSTIDYAAYAIHTVISGEYENHRDEKLNMLALRNMMGNAGCVAYEEGL
                     SVLYTTNWQNEDIRLLALKLRQANALPSLTLLLHNDAFEGESDYLMHIAAASYVDFII
                     NSQGKESFLAQYSQKPVITAELETAWYQYIDDFGKRIRIAPKPKPALPEHLKGFNFAH
                     EGYQIYNGYMGSEAEHSIAKLKEIGANTIAIIPYSGFQSMDKPYRFRYTNGPGAENDA
                     AVVHAGYVAHQQGMVTMLKPQLWSWLGWTGDIKMQNAADWDLFFEYYEEWIMHYALLA
                     ELYDYDILCIGNEFQAATLSEHARWDALFEKVRLVYHGNITYAANWGSEFEQAGFWDM
                     LDFISVNCYYPLSEKTSPTDAELLAAFEHNLDIIEKVQKKYNKPVIFTEIGFKSIASP
                     WIAPHKDADEQAYDETSQDRCYQVMFKAMADEPWIKGTYIWKWPSYMDFSKEYSKDFN
                     PCNKLAEKTISENYKKQ"
     gene            complement(15407..16033)
                     /locus_tag="PLL28_05575"
     CDS             complement(15407..16033)
                     /locus_tag="PLL28_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68825.1"
                     /translation="MIKRIKYAVLALVLTMSLTVKADSPITSTYIATFYYEYPIVGIA
                     ETTRGLNDEIAAYLLDPDNLIDVKAAVINAIGWNYDGTQNADLFKTYLAKAHNTTTQQ
                     LSMSSLSGDELFCLGYFMAMDNYFVVDDAINVLQMAADKNSTSFTTHLFLGMVQAQKA
                     MDYDFCQVWRITASVINDKSLTRDLKTGAIQSIVDYMILYKGACVADY"
     gene            complement(16026..16655)
                     /locus_tag="PLL28_05580"
     CDS             complement(16026..16655)
                     /locus_tag="PLL28_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68826.1"
                     /translation="MPDNTYVLVAARMTDEDLLEALAHHEKYVDDMLTAMLDVAEKRG
                     LTVLNAEALRAEIAGRIEPEEILQAIDEERPIPIELPELYSQTAILAFSIFFSPAFGG
                     ILFAMNMKRMQKKNGWLVVLLSFAVSAISGYVTWYYAKGTFLGILIPIACGLLLSEIL
                     WSRIFGKHQPYKHRRVIIPLVIALAIAIPLSYYMYEHPEQFGPQTTQHD"
     gene            16682..17551
                     /locus_tag="PLL28_05585"
     CDS             16682..17551
                     /locus_tag="PLL28_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68827.1"
                     /translation="MNKSVKLLLIICFFTALPAFAQTQLNTKGLFTFVRVLPIPSDFE
                     IGPGQINKDGSSFFVGLTDGDLENMEDLHSDVYRVNVTSDVNAIPITGLEIPNAIDSL
                     RFFQASASDNDAYIVFVVNAWAGWNDNELAIVKRQPSGAFGGMRMLTEVNKEHFSDAY
                     PWLSGDANRLYFSQDFKLMYSERSSPDSLFSSPKPVDFVGDVQLEIVSAWLPPDEKSI
                     FLLANNRIYKSTRKSTTEPFSFPSLWTDEFKDFYFVAGLSFAPDKKTMYIYHSDETSQ
                     HILQYQLSKGKAW"
     gene            17677..18087
                     /locus_tag="PLL28_05590"
     CDS             17677..18087
                     /locus_tag="PLL28_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68828.1"
                     /translation="MPPTLYPDKSISEIAQMAYTDSLHHSFLMVLRDVTPDLEEDSIF
                     ITHQRYHVYACAGIESAMLNADKDDTDSVMTGGNLVYTTPIEGEFGGHEVHYRVSTIE
                     TEYYFYQILTWHLADNDAMEKQLYDAMLSFRPFR"
     gene            18108..18851
                     /locus_tag="PLL28_05595"
     CDS             18108..18851
                     /locus_tag="PLL28_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68829.1"
                     /translation="MINFKNTGFAVIAILMTASFISCGDENQADEKTPAPLNRSMFYG
                     KITYTSTTSSPDSVFLATMSTFSPHAVDIYFGKSAFRMVEHGGLSNGNILVLPESGEA
                     WQLDSTKMIAYLGEYSDLGDPSDALKDLMPDHFAPTVKATGKTANILGIPCTEYYIVR
                     SGIIPKEDSATIMVANTINFPSSRYDVQTEINHAAVPAPLFLGYEDGAIMQMTVVNKR
                     YTRKFEITALQENYFPEGIFDIPANFQKK"
     gene            18848..>19518
                     /locus_tag="PLL28_05600"
     CDS             18848..>19518
                     /locus_tag="PLL28_05600"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HNF68830.1"
                     /translation="MNRLKLLTGSFFLAQTLMAQSVQVDSVTAPSSFRGMAVVSDAEI
                     WISGSKGYIGHSIDSGKTYTFHQIPKYENAEIRDIEFLNDGALVAMSSTQPAAILRST
                     DKGATWKEVFRRDTSAYFLDAIDFSEKQGTCLGDPINGHFLILHTSNYGKTWTETYTN
                     IATDSLAAFAASGSTLQYLSTNEIVFGTGGKEALLFYSKDAGKTWETTEMLMNSGKES
                     AGIFSV"
BASE COUNT         5863 a         3972 c         4043 g         5640 t
ORIGIN      
        1 tgaattatgg aaaaaagata ctgttatgta tgcgaatatt cttgcatata cagaaggtat
       61 caatgccttt atagatcctt tgcttgaaga gggatatgaa gcctacccta ttgaatttaa
      121 attgctggac tacaagcctg aagaatggac gccgctcaat tctgcacttc tgttgcaata
      181 tatgtcgagc acgctcacag gatttgatga cgacctcgaa tattctaact tgcgcaaact
      241 actcacacct gaagaatttg ctgcattgtt ccctgatcgc cctgaaggaa ttgatccgat
      301 aattccaagc gaaaaagttt acaatttcac tgttaatgat gctgattcta taccacaaga
      361 tgccagaaca ggctatttta aaaaggtgct ggaaattttt gagagtcagc atgatcatac
      421 acaaataggc agcaacaatt gggctgttga cagcagcaga tcgaaaagcg gtcaccccat
      481 ccttgccaat gatccgcatc tgcaattaaa tctgccttcc ttgtggtttg aaattcaatt
      541 gcagacacct gcaacaaact gctatggggt atcgctaccg ggagctcctg caattgtcat
      601 cggatttaat gaaaatatca gctggggcgt taccaatgct ggaatagatg tgcgcgactg
      661 gtataccatt gaatataaag acgataccaa atcagcatac aaatacggaa aaacctggaa
      721 aaaaactgag caacgtatcg aaacaattga tgtgcgcaat gcagatccaa tcattgacac
      781 tgtgatttac acgcattatg gtcctattgt gtatgaagat gaagaaataa ctgccggcac
      841 aaaacaagat ccacataaag tgcaacgcat agacctggca atgaaatggg ctgctcttga
      901 accgggagaa caaatcaaaa cattctttta tctcaataga gcaaaaaatt ataacgatta
      961 tcgtcaggct atcgcctatt tcaattgccc tgcacagaat tttgttttcg cctcacataa
     1021 tggtgatatt gccatcacac aacaaggtaa attcgccgtt aagaaaaaag aacaaggtcg
     1081 ctttgtttta aacggcagcg atcctggaga tgattatacc gatttcattc ctttcgatcg
     1141 caatcccggt attttaaatc cgatgcgcgg attttgttct tcagcaaatc aacatcctac
     1201 agatgcaacg tatccctatt attatacagg atttgatttt gagttttatc gcaatcgcat
     1261 catcaatcgc agccttgatg ccatgcatga tgccactatc gaaagcatgg agaaattgca
     1321 acagaacaat tacaatctgc atgcaagcga agctttacct ctcatgctag ataaattgac
     1381 ctacgatgtt ggcacaccgg attcaacagc atacactgta aagacagcat tgtcggaatg
     1441 gaatttcatg aacgatgctg acatgctcgc cccaacttat ttccagatgt ggtgggatac
     1501 attgtatgcg atgatgtggg atgaaatgga agactctgtg atagcatacg tgcggccgaa
     1561 taaatacaat accgtaaaag cgatgcatgc ctttccgaat gatcagcgct tgtttgatat
     1621 gatttctaca cctgatcaaa aagaaacact cgatgaactt atcaatttga gttatggtat
     1681 catggtgcat atggtagatt ccctcgccgc tgaagataaa aatctgttgc aatggtatcg
     1741 acataaacga actaatatta tgcatatcag tcagattccg gccttcagta aaatgtcggt
     1801 gcacaatggc ggttatcaaa gtatagtcaa tgccaccagc gaaacgcatg gtccaagttg
     1861 gagaatgata gtagaaatgt cgaatcctgt taaagcggtg ggtgtatatc cgggaggtca
     1921 atcaggcaat cccggaagta aatactacga caattttgtc gacgactggg cagcaggaag
     1981 atattatgaa ttgcatcttt accagagtga agaagaagca aaagaacaag cacaatttac
     2041 gatgcgattt aattgatatg aagtattttg taagtatcac cataggcttt accttgtgta
     2101 tggcaggaca gttcttcctg ccatggtggt ggatatttgc accgcttacc ttcctcattt
     2161 cctttattgt aaattatcat tcagcctggc gtgcattttt tgcaggcttg ttaattgtgt
     2221 ttatttcctg gatggctgct tatatttata aagatcttgc caacgatagt attatgtcag
     2281 gcaaaatggc caatctcttc aatttcaaaa ataattacgc gctctttttt atagtagcta
     2341 ttgccatggg aattattggt ggcctcactt ctaccgcagc ctatttcact cgaaattcgt
     2401 aaaagatgtc tgattttgat gtgttgctga tcacggaaga tcgttatgaa tcaccgcaac
     2461 atatcgatgg atacattgcc aatattttgc aagatgatgc cattctgact gcagcatttg
     2521 aagaggtagg attgcgcacg aaacgcgttt catggagtaa tccggagatc gattggtctg
     2581 cttcccgctg cggcttgttt agaactacct gggattattt tcttcgcttc aaccagttta
     2641 agaactggat ggagcaaact gcacaaaagg taatttttat caatccaata aaagtcattc
     2701 gttggaatat ggataaacat tatcttgctg atctaaaagc tacaggcatt cgcgtggttg
     2761 acacgacttt tattgagcaa aacacttcat gcgatcttgt tgcattgctg caggcatccg
     2821 gtgccgattc gggtattgtc aagcccgctg tatctggcac tgcgcgacat acctatgttg
     2881 tagataattc aagtctcgaa agcgtgcagc ccattgtaaa tgatttgttg aagagcgagg
     2941 cgctgttgtt tcaaccattt cagaaagaca ttttattaaa cggagaatta tcgctgatca
     3001 tcatcgacgg taaatgcacg catgcagtaa gaaagactgc aaagcagggc gattttcgtg
     3061 tgcaggatga ttttggtggc actgtgcatg atcatatacc aacaccggaa gaaattcaat
     3121 ttgctgaatc agcagttgcc gcctgtccgg agaaaccatt gtatgcacgc gtagatatcg
     3181 tgcgcaacaa tgccggtgac ctggccatta tggaactcga attaatagaa ccggaattat
     3241 ggtttagaaa aaatcctgct gctgcaaagc cattagctgc agcaataaaa aacatttatt
     3301 tctcatgaat aaattattca tcacatcttg tttaatcctc attgcaaata tttcaatgca
     3361 tgcacaagtt tcaagtgact atgccgtttt actaagcgct gaaacttctg ttgctcctcc
     3421 aacaattacc ttaaactggc cggcatatgc cgatgcttct cagtacgagg tatttcgcaa
     3481 agcagccgac gatgttagct ggggcacacc aattgcaata cttgccggcg atgcaactgc
     3541 ttatgatgac accgatgtta ttatcgattc gatgtatgaa tacaaagtaa aaagaacgaa
     3601 tatttccgga cttgagggtg agggttattt actcggtgcg attacacttc atgctgtcga
     3661 ttatcgcgga acttgtttgc tggtaatgga tactaccgtt acagatgcat tgacaaatga
     3721 aatcacaaga ttgatggatg atatcagtgg cgatggatgg agtgtttcac aattacatgt
     3781 ggcaagagat atgtcgccga tagctgttcg tgaagctatt attgctgctg cagaagcaga
     3841 tccggagatg caatcggtaa tattaattgg tcgcgttcct gttttgtatt ccggcaattt
     3901 atatccagat ggtcaccccg atcatcaagg tgcctggcct gcagatggta tttatggcga
     3961 tatcaatgca gattataccg atgtcaccat caataccacc acagcaggtc gacctgaaaa
     4021 ttggaataca cccggtgatg gaaaatatga tcaatcgata tttaaatcaa aaatagaatt
     4081 agcagtcggc agaattgaca tgtacaatat gccttcattt gcattagatg aaaccggttt
     4141 attaaaaagg tatctcgaca atgatcacaa attcagaaca ggtatcacac attttgaaca
     4201 gcgggcatta attgatgata atttcggggc ttttgcggga gaagcatttg caagcagtgg
     4261 atggaggagc tatgcacctc ttgtaggaaa tgataatcta caagagttag attatttttc
     4321 caccatgtac gacaacagtt acatctgcag ttatggctgc ggaggtggct ggtttcaggg
     4381 agcaggtggt gtcggatcta caaccgattt cacactcgat accgtgaaaa cagtatttac
     4441 atttttgttt ggaagttatt ttggcgactg ggatgtaaat gacagttttt taagggcacc
     4501 attagcggga ggatatgcgc tcactaatgc atgggcaggc agaccgcatt ggagtttaca
     4561 ttacatggga ctcggatatc ctataggata tgcatcgcga caaacacaaa ataatcaatc
     4621 gacttacaca tccaatattt tccccaaatg ggtgcacatc gcactcatgg gcgaccctac
     4681 ccttcgcatg caagtgatag caccggtttc ggaaatgcat tgcgaaattt cagatattca
     4741 aaatgccatc gatatacatt ttacaacatc tgccgatcct gaagttatcg ggtattatgt
     4801 gtatcgcgca gcaacaacct atggaaaata tgaaagaatt tctgatgaaa ttgtggccac
     4861 ttcaccctac accgacctca caccaattga cggtttgaat tattatcagg taagacctgt
     4921 aatgctaaca acaacaccta gcggaacgtt ttacaatacc ggcattggct tgcgcgatag
     4981 tatttcgatt gatgttactg atgtaaatac attggcacct tcgattgtac atctttatcc
     5041 atcacctgca gatacctata ttgtagtggt gacctcatcc gatctcgtag gatcaacttt
     5101 cataatatat gatatttcag gaaaagctgt gcttacggtt aaactattat ctgaagatac
     5161 aagtataaaa actactaccc tgcctgatgg cgtctatacg ctaaaattca acaacggtat
     5221 aaccaggcaa tttgtagtca gtcattaata atccatacaa cggaatatac ttctttacgc
     5281 gggttccatt tatgtcaatc gaattcctcg atcagtatac ccattttacc catttgataa
     5341 tcgcgcatag cctccagaat ttcggtttgc gaattcatta cgaatggtcc gtattgtatc
     5401 accggttcgt cgatcggttt gcccgataga attaagaaaa tagtttcctg atcggcagtt
     5461 aattcaattc cggctccatc ttggtgcaat acaaccatat gcaatgcagg cacaggtccg
     5521 tatccggtca tggttaatcc gcctttcacc acatacaata atgtttgatg atcggtcggg
     5581 atttcaaatt gacgctttat tcctgccttc attgttacat ttagcatcag caggtccagt
     5641 tttgaatgcg caggcccttt caccccttca aaagttccgg caacaacatg tatgttactt
     5701 ccaacatcat caatggaaat tgacggaata tcattttcct gcaaagcctg ataattgggt
     5761 tgatccattt ttctggctgc aggcgtattc acccaaagct gcactatttc ctgcgaacca
     5821 cctttttctg caagttcttt cgatgggcgt tcgctatgca taatacccat tccggcatcc
     5881 atccattgca caccaccggc atgcacaata gcagaatttc ccctgctgtc gcgatgatgc
     5941 acatcgcctt tgaaaataaa ggtaaccgga ctgaatccac gatgcggatg cggccccact
     6001 cctacctcgc gcggataagc tccggcagca attttcgagc tgtggtggtg caatagcaga
     6061 aagggatcca cctggtctat ggcagctgaa ggcaaaggtt gacgcacggg cattccaccc
     6121 atatccatca tatcggcata ttgcaaagca cgaactgttc tgagtttcat catgctaaca
     6181 taattgaaaa aaacttatga aagttcatta tatgtgtaca catgtttttt gaacatatta
     6241 aagcaattgc tatattttat catagattgt tgagattcct taattttgcg cccaatgcag
     6301 gcgataggtt tttatttatt attaccgttt ttatatttct tttccgtgct accaatgttc
     6361 atattgcgac aattgagcaa tgtggcattt tttatcatgt attacattac cggctaccgc
     6421 cgtgaagtgg tgatgcggaa tttgggacat gcattccctg agaaaagtga gcaggattta
     6481 aaaaagcttg cgcgaggttt ctatttgtat ttgagtgaca cgcttgtaga aacaataaaa
     6541 tcatttacta tctcagacgc cggtttgcgg aaatattgcg ccataaataa ttatgatgtt
     6601 gtacaggcag agattgacaa gggtcgcaat gttatgtttg ttttagggca ttatggcaat
     6661 tgggaatggg gaggcggagc attggcaaaa cgagcgccta ttccgctgaa agccgcctat
     6721 aaaccgcttt cgaatcccta tttcgaaaag ttgatgttcc acacgcgcac aaggcttaat
     6781 atccgattaa ttgccaagcg caagatattg gcacatttga aagatcatgc cgatgagcaa
     6841 accttattgg tgatgattgc cgatcaatcg ccaaatccta aggaacactt ttgggtgaaa
     6901 ttcctcaatc aggatactgc agtggttaca ggcatggaaa aaatcgcacg gcaatataac
     6961 tatacggtaa tttattctac catcgccagt gttggtcgca ataaatatga aattcggttc
     7021 aaaaaaatca ccgacgaccc gcaatcatta gcaccggaag cattaacagc tatgtttatg
     7081 cacgaattgg agcaggatat ttataagaac cctgctccgt atctttggtc gcatagacgt
     7141 tggaagctaa aaaaaccgca ggcttaatcg aataatgtat atccaacagt aaattgaaat
     7201 aagctcttgc gggtatctac ttccagcata cctttttctg tttccacata ttccttaaac
     7261 ttgctcaggt tgccttcata cctagcatcg aaggtgagtt tgttgatgtc gaagttgata
     7321 ccggtttgat atccgacagt catatctttc aaactgaaca agggatcgct catagtgctt
     7381 ctgaccgttg tgaataccgg tcctgccatc aatttcactg atccgaaggc ttgataccca
     7441 agtaatatcg gcacatcaca tttatctaca gacatgctga ataaatcgct caaatccatt
     7501 tcattcacat tggcgtcagc aagtgtcaca agagattgat tttcactgaa tagaatttcc
     7561 ggttgcacga tccacctgcc gaaagtagct cttgcaaatc cgccaaatac caatgccgat
     7621 cctttttgac tttctacatc agccacatca acaccatcga gactcaccac atttagaatt
     7681 acagattgtg catagttgac accacctttc actccgagtt catattcctg tgcctgcaga
     7741 tccactgcag caacacaaaa caggatcagc attattcttt tcataacctt cgatttacag
     7801 gtaggtaaac gaaaactgtg ccacaaagca catcgctcat gacgactact tgtaaatagg
     7861 ctatttttgg acattaaaat caaataatat gtctgcaact ccggaatgga acatacgact
     7921 ggaacccaga aatatcaagt atttgagtgg tccgcggtct agatggcgcg aattatggtg
     7981 ggcattttcg gtatttaccg aatttatcaa aggatttaga gccttgcact ttataggccc
     8041 ctgtattacc gtttttggat ctgccagatt taagcctgat catcaatatt acaagcaagc
     8101 ctatgatatc ggcaagaaaa tgagtgcgtt gggattaaca gtgctgacag gtggtggtcc
     8161 gggaattatg gaagcagcca acaaaggcgc atttgaaaat caggggcgtt ccgtaggctg
     8221 caatattgta ttgccgcagg aacaaaaacc taatccgtac atgcataaat gggtgactat
     8281 caagtacttt ttcgttagaa aggtcttatt ggttaaatac agctacggat ttgtggtaat
     8341 gccgggagga gtcggaacca tggatgaatt ttttgagaca ttgaccttga tacagacagc
     8401 tacgattcat aatttccctg tggtgatgat cgggaaggat tattacaaag acatttggga
     8461 aatgctgcaa aagatgacac aggcaggcac cgtatctccg gaggacttga atctggtgct
     8521 ttttaccgat aatgcgcaag aggctgtaaa ccacattgat ttttacatca aaaagcatta
     8581 ttcctttaaa aaagttaaac ctcgcaaact tcttttagag agatttgttt agaagtaact
     8641 ttgtttggca atgcgcattc ctgaaaagac aatgatgtgg cttaaacgct ttggttgggc
     8701 gggatttttc ttcttcctta tcaagggcat cgtttggctc attgcaggtg ctgccattat
     8761 tgaatggttt cagaaaacct tttaagcgct ctcctaccct gttcatcccg gttttactcc
     8821 cgttcatcac ccttcattaa attaatatat gcgtgcttcc gtgctttgtg gcagaaatca
     8881 aaacatatga aacaaataac gctattcttg ttttgcctga ccatagggac ttttgcccgg
     8941 gcacaacagc tcacacaaac gattcgcgga caggtgacgg ataatcaatc gaaagccacg
     9001 cttccgggag tgaatatcat ggttttagac agcgacccta tattaggtac tacatcagat
     9061 ctcgatggct atttcgcgat cgaacaagtg ccaattggtc gggtaagtat acaggccaca
     9121 tttattggtt atgaaccatt tattgtaaat aatcttgaac ttacgtcagg caaggagttg
     9181 atgctcaatt tcagcatgtc ggaacaagta atcactatga atgaagtagt tgttcgtgcg
     9241 aaagatgaca aaatggaaac gaataacgag atgaccactg tcagcgcgcg acaattcact
     9301 attgaagaaa gtatgcgcta tgccggagcg agaaatgatg tgagtcgcat ggcacagaat
     9361 ttcgccggtg tgcgcggatc taatgatgcc gtaaatgata ttgttattcg cggtaactca
     9421 ccggttggtg tattatggcg tcttgagggg attgatatcc caaatccaaa ccatttcggc
     9481 gacctcggtt ccacaggtgg tccggtttcg atgctgaata ataatattct ggctaacagc
     9541 gacttcttaa caggcgcttt tccggcagag tatggcaatg gtatcagcgg tgtttttgat
     9601 ttgaaaatgc gcaatggcaa ttacaaaaaa catgaatttt taggtcaggt tgggttgaat
     9661 ggatttgaat ttggtgctga aggcccttta agtaaggata gcaaatcttc ataccttatg
     9721 aattatcgct actctacact cggattgatg agtgctatcg gcataaattt cggaacagga
     9781 actgccattc cttattacca ggatattacc ttccgcgtga atatgcccac tagaaaagca
     9841 ggaacctttg ctttgtttgg gcttggcggc gtgagttcga ttgatttaat ttctagttca
     9901 acagaagatt ccgttgacaa tttatataat aatgatctcg acatttatga taaatcaaaa
     9961 attggcatgg ttggatttac gcatcaatat ctgatcaatg ataaatcata taccagattt
    10021 acactttcca cctcatccat tgaaaatact gatattattg attctatatc gagcgaaaat
    10081 tttgaaccga ttaattacta caatcaaaat ttcacccagc ataaagcatc agccagtctg
    10141 atgtataaaa ttaagctcaa cagcagacaa aatctgcagg caggagctag tgcagatatg
    10201 ttgtttgcag atttaaatga tagtatttat cgcaacacat tagatcgctg gatcaccatt
    10261 acttcctata acggcaatac catgttattg caaggttatg tgcaatatca gataaggctt
    10321 acagaaaacc tgactgcaac cgcaggtgtt catgaccaat ttcttgcact taatggcagt
    10381 aattccgttg aaccaagggc aggtatgaaa tacaccatta atgaaaaaaa tgccatcagc
    10441 ttcggttatg gattgcacag tatgatggcg cctattagtg tatatttcaa aacagttgaa
    10501 accactcccg gaaattacat cacaccaaac gacagcattg attttattaa agcacatcac
    10561 tttgttcttg gttacgacag acaattatca agcaccatgc gattcaggtc tgaaatatat
    10621 tatcaacatc tatatgatgt attgatcgat cgcgacagtt cttcttattc tgtattaaat
    10681 accggcagta tgacatttgg ttcaccagat tacctcgtaa ataaagggac aggtcgcaat
    10741 tatggtgcag aggttacgtt agaaaagttt ttggataagg gattatattt ccttgtcacc
    10801 ggttccatat ataaatcgga atatctaggc agcgatgata ttgaacgtga aactgcattc
    10861 tcgggaaatt atgttgccaa tgtggtaggt ggaaaagaat tcgccatact ggccggaaga
    10921 gcaggtgcta aaacgaaaaa atcggtggtg tttgacacca aactaaccat ggccggtggt
    10981 cagcgataca cgccaataga tcttactgct tctatagatg agggtaaaac cgtttacgat
    11041 tgggataagg catttgaagc acaattcgac gattatttcc gctgggatgt aaggctagca
    11101 tttaaaatgg atggcaaaca tgcatcgcag gaatgggcta ttgatgtgca gaatgttaca
    11161 aatgaacaaa atcctttata tcaaagatat aatccggttt cacaaacagt aaatactatt
    11221 tatcaattag gtatctttcc ggttccgcaa tatcgcatca ccttttgagc aaacaatctt
    11281 atataccatt cagaaaggcg ctggcaggca cattagttgt gtcgctggtg cctttaattt
    11341 atcagatact ggtttactac atcgtaggca cacccaaatg tcctgaatta accttttggg
    11401 aaaatgccat gtggaccgct gtgttctcct tgctcgtaac ctttgcgttg tttatagtaa
    11461 acatctacat caatacaaaa ggtcatgaat tactggagga aaaagtaaat gatggcatca
    11521 gaattttcgt gacggtattc gtttcgctac ttgcctcgaa tattgtcatt tttgtgatgt
    11581 ggaaattgtt taatgacctg atattacatc ttccagaaga tggagaaacg gcgcgtgttt
    11641 tctctaatca aatattagca acggtactgg ttatcattgt cgatttgatc attgaggtgg
    11701 atatttatgt caagaaactt aaaatcagta tggaaacaca agcgcgcctg gaaaaagaat
    11761 tcattcgcgc acaactggag agcttaaaga cgcaaattag tccgcacttt ctatttaaca
    11821 gcttcaatgc cttacaatca ttaatcgacc aatctcctgt acaagcaaag tcttttatcc
    11881 aggaactggc gaaggtttat cgatatgtgc tggataaaaa ggacgaaatg gtagtgcctg
    11941 tttccgatga agttgccttt atccgcagtt ttctttattt aaatcatatt cgctttggcg
    12001 acagcttatc cgctgatata gatttatcgg cacaggaaat ggacgggttt atcccgcctt
    12061 taacgctgca attacttgta gaaaatgcca ttaaacacaa tatcatatca caagcgcgac
    12121 cattgcatat cagtgttttt cgcgatgcgg gaaatatcat catcaaaaac aacctgcaac
    12181 tgcgagagga cactccttct gcctcaggca tcggccagtc gaatttggtg gcacgatata
    12241 atttgataag cgacagaaca ccttcattca acattgtaga cgatgcttat attgcaaagg
    12301 ttccggtatt agaacaagaa ttataaggtc atgcacgtat taattattga agacgaatcc
    12361 cttgctgcaa attggttgcg aacacaaatt gagcaatcta caaaagatat gactgtagta
    12421 gccgtactgg acagcgtgtc tgcatctgta gattggcttc aaaaaaacca ggtcgattta
    12481 attttttcag atattcattt agcagatggt ataagttttt ccatttttga acgcatagct
    12541 atttcaaccc ccatcatttt cactaccgct tatgaccatt atgccattcg cgcgtttcag
    12601 gtgaatagta tcgattattt gttaaaacct atagatacag aagccctttc ggcgtcaata
    12661 gaaaaatatc gcagcctgca taccaacatt caaccggatt ggaaaatgct ggtaagcggc
    12721 ctgcaaccaa aaaacaatta tcaggagcgt tttcttgtca gcaccggaca aaaattgaaa
    12781 tcacttccca ttatcgatat cgcctatttc tttgcagata acaagttgaa ttttgtttgt
    12841 gacacgaacg gtcgcaccta tattctggat caaacactgg acaaacttga gcaggttgta
    12901 gatcccggaa cattcttccg catcaacagg cagtttatag tgtcatttgg cgctattcaa
    12961 gatatgttgc actactcaaa gtcacgcgta aagctcactt tgcatccctc aactgagcac
    13021 gacactatag tttctgctga aaggtcagcc gcatttaagg cctggcttaa tcgttaagag
    13081 cctttgtttt agcatgtatg cgggcaatta cactaacttt gtttcatgaa atattggatg
    13141 ctcctttcgg ccattgccct agcctcatgt tcagctactt tagaaaatga acctatcaca
    13201 cacacaaatt ggcctacaag agaagaactg ttccagaatt ttcgaaacca ggacactgct
    13261 ataatagtgt atggaaagga agattataag atttctgatt tattggtgaa tggcgtagcg
    13321 gcgctggctc cgaaagatga tcgcttcacg gtgattacac gcagagacga taaactgaca
    13381 gaatttgagg ctgctaatta tccgttatat atcatcggca ccagaaacaa tcttttgata
    13441 aaacgcgtta gcgaggcaat tcctgtgaaa gtttttgagg gtggatttaa tttcaatgga
    13501 acggattata tggcgcccaa tgatgtgatc aaactttcat tttatcccaa tgtattcaac
    13561 ccgaaaattc catttaccgt tatcacaggc aatcacgaag aagccattgt gcaatatgtg
    13621 cagcaggaga tgaatggcga gtggggatat ttcttttggg ataattgggg ttatcaggtg
    13681 taccataatg gcagacgaac aatattgggt aatttcagtc aagacagtgc ttcgctatgg
    13741 agtattgata aaaagcaaca ttgggaattt gattttgaag gtaaaagttt aaaaacctcg
    13801 ggccaggttc attataccgg acacaatgat ccaaacgtta ccaacttaga caattgggac
    13861 gctgtagcac aagatgacct cactcgtata tcggaattct gcggaaaaaa gataagtgcc
    13921 agattagagg tgcacctcta ccccactccg gaaacaaaaa ctttgttgat gagtgatccg
    13981 gatcagtcga caattgatta tgccgcttat gccatacata ctgtaatttc cggtgaatat
    14041 gaaaaccacc gcgacgagaa attaaatatg cttgcactgc gcaacatgat ggggaatgcc
    14101 ggatgtgttg catatgaaga aggactatcc gttttatata ccactaactg gcaaaatgag
    14161 gatataaggt tgctggcatt aaaacttcgc caggcaaatg cacttccatc gttgacactt
    14221 ttattacata atgatgcatt tgaaggcgaa tcggattatc tgatgcatat tgccgctgcc
    14281 agttatgtcg attttatcat caatagtcag ggaaaggaaa gcttccttgc tcaatacagt
    14341 caaaagccgg taattactgc tgaattagaa actgcctggt atcaatatat agatgatttc
    14401 ggcaagcgta ttcgaattgc tccgaagcca aaacccgcac taccggagca tcttaagggc
    14461 tttaattttg ctcatgaagg atatcagata tacaatggct atatgggttc agaagctgaa
    14521 cattctatcg ccaaactaaa agaaattggt gcaaatacta ttgctattat tccatatagc
    14581 ggatttcagt cgatggataa accttatcgc tttcgctaca ctaatggacc cggcgcggaa
    14641 aatgatgcag cagttgtgca tgcaggctat gttgctcatc agcaaggaat ggttaccatg
    14701 ttgaaaccgc aattatggtc ttggttagga tggactggag atattaaaat gcagaatgct
    14761 gctgattggg atttgttttt cgaatattat gaagaatgga tcatgcatta tgctttgtta
    14821 gctgaactat atgattatga tattttgtgc ataggcaatg aatttcaagc cgcaacgctt
    14881 tctgagcatg caagatggga tgccttgttt gaaaaagttc gactagtata tcatggaaat
    14941 atcacctatg cagcaaactg gggttctgaa tttgaacaag ccggattttg ggatatgttg
    15001 gatttcattt cagtgaattg ctactatccg ctaagcgaaa aaacttcacc tactgatgcc
    15061 gaattgttag ctgcttttga gcacaattta gatattatag aaaaggtgca aaagaaatat
    15121 aataaaccgg taatcttcac tgaaatcgga tttaaaagta ttgcttcccc atggatagca
    15181 ccacacaaag atgccgacga acaagcatat gacgaaacat cacaagatcg atgctaccaa
    15241 gtaatgttta aagcaatggc agacgaaccc tggatcaaag gaacctatat ttggaaatgg
    15301 ccaagctata tggatttctc taaagaatac agcaaggatt tcaatccctg caataagctg
    15361 gcagaaaaaa caatctcaga aaattacaaa aagcaataat gtcgtattaa taatcagcaa
    15421 cgcacgcacc tttatacaag atcatgtaat ctacaataga ttgaatggct cctgtcttta
    15481 gatcgcgcgt taaactcttg tcatttatta cggaagccgt gatgcgccat acctgacaga
    15541 aatcgtaatc cattgctttt tgtgcctgca ccatacccag aaataaatgc gtggtgaaac
    15601 ttgtgctgtt tttatcagcc gccatctgaa gaacattgat tgcgtcatct accacaaaat
    15661 aattatccat tgccataaag tatccgaggc aaaacaattc atcaccgctt aatgatgaca
    15721 tggatagttg ctgcgtggta gtattatgtg cttttgcaag atatgtttta aataaatcgg
    15781 cattctgtgt gccatcgtaa ttccacccaa tggcattgat cacggcagct ttcacatcga
    15841 tcagattatc cggatcgagc aaatacgctg ctatctcatc gtttaatccg cgcgttgttt
    15901 cagcgattcc cactatggga tattcgtaat aaaatgttgc aatataggtt gaggtaatcg
    15961 gcgaatctgc ttttactgtt aatgacattg tcagcactaa agccaaaaca gcatacttaa
    16021 tccttttaat catgttgtgt agtttgaggt ccaaattgtt cgggatgttc atacatgtaa
    16081 taactcaatg gaatggcaat agccaatgca attaccaatg gaataattac ccttctgtgt
    16141 ttataaggtt gatgttttcc aaaaatgcgc gaccataaaa tttcagataa tagtaatccg
    16201 caggctatgg gaatcagtat gcctaaaaaa gtgcctttgg cataatacca ggtaacgtaa
    16261 ccgctgatgg cagaaacggc aaaactcagc aatacgacca gccagccatt tttcttttgc
    16321 atgcgcttca tgttcatggc aaaaagtata ccaccaaatg caggagaaaa gaatatcgaa
    16381 aaggcaagaa tcgcagtttg ggaataaagt tcgggtaact cgataggtat tggtcgctct
    16441 tcatcaatag cctggagtat ttcttccggt tcgatgcgac cggcaatttc agcgcgcaag
    16501 gcctcagcat ttaacacggt caggccacgt ttttcggcaa catcgagcat agcagtgagc
    16561 atatcatcta catacttctc atggtgtgcc agggcctcca gcaggtcttc atcggtcatt
    16621 cgggccgcaa cgagcacata tgtattatcc ggcataattt ttaataccta attttgtcat
    16681 tatgaacaag agtgtaaagt tattgctaat tatctgcttt ttcacagccc tgccggcatt
    16741 tgcccaaaca caactcaata ccaagggact gttcaccttt gtcagggtgc ttcccatccc
    16801 atcagatttt gagatcggac ccgggcaaat caataaagat ggcagttcat tctttgtggg
    16861 ccttaccgat ggcgacctgg aaaatatgga agacctgcat tcagatgtat atcgcgtgaa
    16921 tgttacttct gatgtaaatg caattccaat taccggttta gagatcccta atgccatcga
    16981 cagccttcgt ttttttcagg cttccgcttc agataacgat gcctatatcg tttttgttgt
    17041 aaatgcctgg gcaggatgga atgacaatga actggccatt gtgaaacgac aaccatcagg
    17101 agcttttggc ggaatgcgca tgcttaccga ggtaaataaa gaacattttt ctgatgctta
    17161 tccgtggctt tccggtgatg caaatcgtct gtatttttca caggatttta aattgatgta
    17221 cagcgaacgc agtagtcccg attcactatt ttcttccccc aaacctgtcg attttgttgg
    17281 tgatgtgcaa ttggaaattg taagtgcatg gcttccacct gatgaaaaat caatattctt
    17341 actcgcaaac aatcgcattt ataaatcaac gcgtaaatca actactgagc ctttctcatt
    17401 tccctccttg tggacggatg agtttaaaga tttttatttt gtagcaggat tgagttttgc
    17461 accggataaa aaaacgatgt atatttatca ttctgacgaa acatcacaac atattttaca
    17521 atatcagtta agcaaaggaa aggcctggta agactacaaa acatatgttg cctgttggtg
    17581 ttgccaataa tatggcaatc ctgctcgaaa acaatcgagc cgcccgatta cagcaatttc
    17641 attcacgttc aaaaaaaggg gtctttcgaa atttatttgc cccctactct atatcctgat
    17701 aaatctatca gcgaaattgc acaaatggca tataccgatt cgctgcatca tagtttttta
    17761 atggtgctgc gcgatgtcac tcccgatctt gaagaagata gtatttttat tacacatcaa
    17821 cgctatcatg tatatgcttg tgccggaatt gaaagtgcta tgctaaatgc tgataaagat
    17881 gatactgata gtgtcatgac cggtgggaac ttagtatata ctacgccgat tgaaggagaa
    17941 ttcggtgggc atgaagtgca ttatcgtgta tccactatag aaacagaata ttatttctac
    18001 caaatactca catggcatct cgcagacaat gacgcgatgg aaaagcaatt atatgatgct
    18061 atgttaagtt ttcgaccttt caggtgataa ggtcttatct ttgcctgatg atcaacttca
    18121 aaaacacagg attcgcagtg attgcaattt tgatgacggc gagtttcatt tcatgtggtg
    18181 atgaaaatca agctgatgaa aaaacacctg caccattgaa tcgcagcatg ttctatggaa
    18241 agattactta tacttccaca acttcatcac ctgattctgt tttccttgca accatgtcga
    18301 ctttcagtcc gcatgctgtt gacatctact tcgggaaatc tgcatttcgc atggtagaac
    18361 acggtggctt atcaaatgga aatattctgg tactacccga atccggcgaa gcatggcaac
    18421 tcgacagcac aaaaatgatc gcatatttag gtgaatattc agaccttgga gacccaagtg
    18481 atgcgcttaa agatctaatg cccgatcatt tcgctcctac tgtaaaagct acaggcaaaa
    18541 ccgccaatat tcttggcatt ccatgcacgg aatattatat cgttcgttca ggtatcattc
    18601 cgaaggaaga cagcgctaca atcatggtag ccaatactat taatttccct tcatcacgct
    18661 atgatgtgca aactgaaatc aatcatgctg ccgttcctgc accgcttttt cttggttatg
    18721 aagatggcgc tatcatgcaa atgacagttg taaacaagcg atatacgcgc aaatttgaaa
    18781 taacagcact acaggaaaat tatttccccg aaggtatatt tgatatcccg gcaaattttc
    18841 agaaaaaatg aacagactca aattgctgac aggtagtttt ttccttgcac aaaccttaat
    18901 ggcacagtct gttcaggtag attctgtaac tgcaccttca agcttccgtg gcatggcagt
    18961 tgtaagcgat gctgaaattt ggataagtgg tagtaaagga tatatcggac atagtatcga
    19021 cagtggtaaa acctatacgt ttcaccaaat tcccaaatac gaaaatgctg aaatccgcga
    19081 tatcgaattt ttaaatgatg gcgctttggt tgctatgagc agcactcagc cagccgctat
    19141 tctaaggtct acagataagg gagctacctg gaaggaagta tttcgtcgcg atacatcagc
    19201 atatttcctt gatgccatag acttcagcga aaaacaaggc acttgtcttg gcgatcctat
    19261 caacggtcat tttctcattc ttcataccag taattatggt aaaacctgga cagagactta
    19321 tacaaatatc gcaaccgata gcctcgcagc ttttgcagca agtggatcaa ctttgcaata
    19381 tttaagtacc aacgaaattg tttttggcac cggaggaaaa gaagccctgt tattttattc
    19441 aaaggatgcc ggaaaaacat gggaaacaac tgaaatgctg atgaattccg gaaaggaaag
    19501 cgcaggtatc ttttcggt
//
LOCUS       DAOQHY010000047        19307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1281_length_19307_cov_5.998701, whole genome shotgun sequence.
ACCESSION   DAOQHY010000047 DAOQHY010000000
VERSION     DAOQHY010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 19307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19307
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1281_length_19307_cov_5.998701"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PLL28_05605"
     CDS             complement(<1..526)
                     /locus_tag="PLL28_05605"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNF68831.1"
                     /translation="MPDNFFSVMQFELVATVIMFIVLLLRLGKKRITGDSLLLVINGL
                     LTINLLVGFFCNAHGSLFGDMFFSNPLICFQKSILNLGLLIISLQSWPWLKNHHHTAE
                     FYIILLASLIGMFFMISAQNMFMVYLSLELATIPLAALANFDLQLRRSGEAAMKYIIS
                     SAFSSGVLLFGISLV"
     gene            complement(535..2046)
                     /locus_tag="PLL28_05610"
     CDS             complement(535..2046)
                     /locus_tag="PLL28_05610"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNF68832.1"
                     /translation="MNLNLLILIPVATAIGIIAVKGLPRVRMVALAGALVQFILSFVL
                     FFSFRAERASGNQDPFVFTQYYKWFPSLNINYQIGVDGISIAMIMLTAFVVLAGVLIS
                     WRQEKWSKEYFLLLILLSTGAYGFFIALDLFTLFFFLEVAVIPKFLLIGIWGSGKKEY
                     AAMKLALMLMGGSALVFVGLAGTYFFGGQSFDLSSGAHITMDPDKQMFFFPFLFVGFG
                     IFTALFPFHTWVPDGHSSAPTAASMFLAGISMKLGGYGCLRAATWLMPEAAHYYQPLI
                     IGLSTIAIIYGAFATLMQTDLKYINAYSSVSHCGFVLLGIGMLNETAINGAVMQMVSH
                     GLMTALFFAAIGMVYDRTHTRMVKDIGGLLKITPFIGTVFILAGLCSLGLPGMSGFVA
                     EMTVFMGGWQNPETLYRVATILACASIVVTAVYILRAAGKTMMGPLVNQEHALFSDAK
                     WNERTAAFVLLTGILIMGLAPFIITDIIYPGTHVLMDKIASGASITNEIELLK"
     gene            complement(2119..4038)
                     /gene="nuoL"
                     /locus_tag="PLL28_05615"
     CDS             complement(2119..4038)
                     /gene="nuoL"
                     /locus_tag="PLL28_05615"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNF68833.1"
                     /translation="MSNYDYISLIPILPIAGFVLIGLAGKKYFPALSGWLATLLIGTS
                     AVLSLFTAYQYFIVDGAANGVYPQLLPVDMQWLQFTNTLSINMGILLDPISVMMLVVV
                     TFVSLMVHIYSTGYMKGESRYATFFAYLQLFTFSMLGLVMSSNLFQIYIFWELVGVSS
                     FLLIGFYFDKPSAVAASKKAFIVTRFADLFFLIGILITGVTAHSFNFTDIIANLTSPE
                     FLKGYSATFLGFSVVTWALVLIFIGGAGKSAMFPLHIWLPDAMEGPTPVSALIHAATM
                     VVAGVYLVARLFPVFAITGSGAIDVVAYIGIFSALFAALIACTQTDIKRVLAYSTMSQ
                     IGYMMFALGVSSQAHDAGFTASMFHLFTHAFFKSLLFLGAGLVIHAVHSNDMKDMGGL
                     RKYLPITHITFLIGCLAIAGVPPFAGFFSKEGILHAAYTTHPDIYYVALVTSGLTAFY
                     MFRLYFRIFWNKADIHLHHFHGEGAWSMKFPLLILATLSIAAGFVPFAHYVTATRTEG
                     HMHTDIMFSIAPVLIGLLGIAVAMMWYKNENDRADKAVAMMRGVYRTAYHKFYIDELY
                     IFITKKVIFNCIGRPAAWIDRNIVDGTMNFIALMTGEISDSIKGMQSGKVQGYAMYFF
                     AGVIGLTLICLYIWM"
     gene            complement(4041..4355)
                     /gene="nuoK"
                     /locus_tag="PLL28_05620"
     CDS             complement(4041..4355)
                     /gene="nuoK"
                     /locus_tag="PLL28_05620"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNF68834.1"
                     /translation="MNNIGIEQILFISTALFFIGVYGLFTRRNMITMLMAVELILNSV
                     NINFVSFNKYLYPERLDGVFFTIFIIAIAAAEAAVAIAIIINIYRNHQSIDVEDSEEM
                     KY"
     gene            complement(4352..4870)
                     /locus_tag="PLL28_05625"
     CDS             complement(4352..4870)
                     /locus_tag="PLL28_05625"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNF68835.1"
                     /translation="MNAYSIIFYLLAAFIIGNGVLAMTTRKIFRAAIFLLFSLIGVAG
                     LYFWMHVEFVAAVQVIVYVGGIVVLIIFSIFLTQQTGKDMPRPTNTRMIFSALAAILG
                     AALTYSLIRQYGFIGGSGDQFNVSPQRIGDQMLDYQDTGYALPFEVISILLLAAMIGC
                     IVIALKSKPQES"
     gene            complement(4923..5393)
                     /locus_tag="PLL28_05630"
     CDS             complement(4923..5393)
                     /locus_tag="PLL28_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNF68836.1"
                     /translation="MFLSKYISEVFGGVKSLLTGMRRTGYYFTHHKNIITQQYPNQKP
                     VLAERFRGEVVMTHDENNEHACTGCTACELACPNGTIKIVTKFDITPEGKKKKAIDTF
                     VYHLELCTMCNLCVDACPTGAITMSNSFEHSVFDRSELTKVLNKPGSKIREGVE"
     gene            complement(5512..6591)
                     /gene="nuoH"
                     /locus_tag="PLL28_05635"
     CDS             complement(5512..6591)
                     /gene="nuoH"
                     /locus_tag="PLL28_05635"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNF68837.1"
                     /translation="MEFSLASIADSIDQWLNAHCASWLALTIEFVIVGVLCIGLFAVL
                     GLVLIMMERKVSAYMQLRLGPNRVGPKGMFQTVADTLKLLVKEGLTPAGADKFLFNLA
                     PFVVMIASMLVLAPIAFAKDFVIWDVNIGVLYVSAVSSIGVIGILMAGWASNNKYSLL
                     GAMRSGAQIVSYELSAGLSILTIVIFSQDMSISSIINSQQDGWWIFKGHIPVIISFVI
                     FIIAVTAETNRAPFDLAEAESELTAGFHTEYSGMKFALFFLAEYVNIFIVCAIGATLY
                     LGGWMPLHIGNWEAFNHIMDYIPSSVWFLGKTFFLIFVIMWFRWTFPRLRIDQLLNLE
                     WKYLLPISMFNLLLATLVAIQGWYF"
     gene            complement(6694..7809)
                     /locus_tag="PLL28_05640"
     CDS             complement(6694..7809)
                     /locus_tag="PLL28_05640"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNF68838.1"
                     /translation="MLQEVAKTDTGDLVINVGPQHPSTHGVLHLVITLNGETIKKVEP
                     HLGYIHRSIEKMCESLSYRQFIYSTSRMDYLSAHINNHGCALAVEKGLQIEVPQRAQV
                     IRVLMDELTRLASHQLWWGALAMDVGAITPFFHAFRERELINDIMEETCGARLTMNYM
                     VPGGVMFDLHPDFQKKVKDFLTLFKSKLPEYDELVTGNIIFQNRMKNVGYISKEDAIS
                     YGCSGPTARASGVSCDIRKKYPYDIYAQLQFSEAIETAGDCWGRYLVRMEEMRQSISI
                     IEQLIDNIPEGDFVAKTKAVLKLPKGEFFTRVETARGELGIYIVSEGGLTPYRIKFRS
                     PGFSNLSALDHMAKGQKIGDLVAIMGTLDLVIPDIDR"
     assembly_gap    8028..8127
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8208..8762)
                     /gene="nuoB"
                     /locus_tag="PLL28_05645"
     CDS             complement(8208..8762)
                     /gene="nuoB"
                     /locus_tag="PLL28_05645"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HNF68839.1"
                     /translation="MDVKEPGYQQQPFPGDVHPTATGGLLVSRLDDVINWARSNSLWP
                     LVFGTSCCAIEMMSTASAKYDWSRFGFEVARATPRQADVIIIAGTIVNKMAPVLKRLY
                     DQMADPKYVIAMGACATSGGPFFYNTYSVVKGADHVIPVDVYVAGCPPRPEALLHALI
                     TLQTKIQAGGKREKIVIEKFYDNQ"
     gene            complement(8753..9103)
                     /locus_tag="PLL28_05650"
     CDS             complement(8753..9103)
                     /locus_tag="PLL28_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNF68840.1"
                     /translation="MGPASLLVLIMAAVAFSTAGILLSKVVLKASKNPVKGEPYECGV
                     PTIGQNWVQFNVGYYLFALIFLIFDVELIFLYPWAVSVQRLGMFAFTEAVIFLFILFM
                     GFLYAHKKGALKWM"
     gene            9640..13215
                     /gene="nifJ"
                     /locus_tag="PLL28_05655"
     CDS             9640..13215
                     /gene="nifJ"
                     /locus_tag="PLL28_05655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNF68841.1"
                     /translation="MSHTESQLISPDVKHGNFNFKRDIMDGNEAAATIAFKTNEVCAI
                     YPITPSSPMGEWADEWSSKGVINIFGQVPTVIEMQSEAGAAGAIHGALAAGALSTTFT
                     CSQGLLLMIPNMFKIAGELTPAVFHIAARAVATHALSIFGDHSDVMAARATGFSMLFG
                     CNSQEVMDMALISQSATLSARVPFMNIFDGFRTSHELSEVEIIPDEIIRAMIKMEDVM
                     AHRSRAMNPANPFIKGTAQNPDVFFQGREASNKYYMQVPEVVKAKMKAFASLTGRTYN
                     LFDYYGHPEAESIIIIMGSGAGPVQEIVDYLNANGGKTGVVIVRLYRPFAIDDFVHAI
                     PSTVTKIAVLDRCKEPGAIGEPLYMDVINALREKERDIRKVIGGRYGLGSKEFNASMA
                     KAVFDELNQPKPKNHFTIGIDDDVTNTSLHFDPDFNLEKQYYFRGLFYGLGADGTVSA
                     NKNSIKIIGDHTGDFVQGYFVYDSKKSGSLTVSHLRAGKNPIRSSYLVQSANFVACHH
                     FNYLLKYDVLRYAEHGAVFLLNTPYPHEEVWQHLPKKIQEEIIEKQLKFFVINASAVA
                     RQAELGTRINTILQTCFFAISNVMSSDEAIADIKQAIHDSYIKKGISVVEKNYAAVDK
                     ALANLFEVDYTKNATGNLEGDKIVPDDAPDFVQKVLARIMAGEGDELPVSAFPVDGRY
                     PAGTTQYEKRNIAEQVPVWDADLCSQCGKCFMICPHAAIRPKVYAKEALADAPPTFRH
                     VAPIGKEFNKEEEAYTLQVSVEDCTGCNLCVAYCPVESKLEKGHKAINMVDKLGLEES
                     EQANWDFFLSLPDIDREKVNKNTVKGTQFLQPLFEFSGACAGCGETPYLKLLTQLFGD
                     RMVVANATGCSSIFGGNLPTTPWTTNHEGQGPAWANSLFEDNAEFGLGIKLATDKKRE
                     IALYLLDQMQPMIDAGLYDAIKSHHGTAESDINIYKQQIAALRKQIRGIDNPNAHMLL
                     HFADYLIEKSIWIIGGDGWAYDIGFSGLDHVLSTGENVNIFVLDTEVYSNTGGQKSKA
                     SPLGASAKFSINGKRSGKKDLALQAIAHGKAYVAQIAMAANDMHTVRTIQEAEAFPGP
                     SLIIAYSHCIAHGYDMCHGSDQQKNAVNSGYWPLFRYNPLKARGERFTMDSKDPSIPL
                     SEFLYHENRFASIRAKDPALAETFLHMAEADLKAKYERLHLLESM"
     gene            13554..14660
                     /locus_tag="PLL28_05660"
     CDS             13554..14660
                     /locus_tag="PLL28_05660"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNF68842.1"
                     /translation="MSTLKKTFFALTLLFATQQFVQAQTTEQAFSPSIKVKALIHSRF
                     EASLTDSIDVAGKYFANPVTTNFRMRRIELRTDIKLNDKFSGVVRVQLPELKGSSVGK
                     VIELAYMDYKYNDMLNVRFGQFKSPFELDEQTSHEDLRMIDRGPTSVMFVNNSLASYQ
                     PGAMVFGTFMKSKTPLSYYLAFVNGSNRAVNNDDNSQKNIVGRLEFVPIKGVKIGANA
                     QQIYTKDTSGLSYGGDLSIIRDLNDKMKLIVEGEYITGYQVNDFNADTTGLEIGDYTM
                     GGYFGQVLLRSELNKSWCKTFEVGGKYEHTDPNTTVDDNAFNTITGGIGFIFTPDNMA
                     RLQLNIVHTDYEKTITPGGLMAANMFVTQFQLKF"
     gene            14779..16692
                     /locus_tag="PLL28_05665"
     CDS             14779..16692
                     /locus_tag="PLL28_05665"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNF68843.1"
                     /translation="MNLKYYIVLLIANSLQLYAFAQSMPDQMVISDDGTRLSVGGQEP
                     NDFYNIDSIRSINLIFDEPNYWSALTANYASKTDLAATMVYNGDTLAEQVGVRFKGQT
                     SYMMNMTDKKSFNITIDYIDSSQDIHGYNTLNLNCGYEDPSFMRESVYENNIRKYIPG
                     LAVNYVHLYLNGEDWGLYLNVQQLNKDYYKEWFMTNDGTSWRAEKEGFGGPGGGDPFG
                     AGYCSLNYLGTDSSDYTPYYQLKSTDLEDPWTDLINTTDVLNNTADASLEDAVNNVLD
                     LDRTLWFLALEIIFSDDDSYVNKGGMDYYVYWEKETDRIVPMEYDGNSCMDPMHLSWT
                     PFYHETDADYALLNVLLGNDNIRQRYLAHMRTILDELFNSDYMDAYIDAMDTRIDALV
                     NADPKKIYTYAQYNADVTELKTFVEEHYNDFINNTEIDRLDPEISETLMYSAAGDWIA
                     PLSMEAPVVTTNVYTESNINGVNLYYGTGLVGRFYPLQMFDDGAHNDGLFNDGTWGAN
                     LPAMPAGTDVRYYIEAIADDGYGTRSYDPVGAEHDVYYYQVRGNVAVEDFIAETINVY
                     PNPATDMVQIILPSSNETADIELFSLGGNSVLNQLNYKSGAPLNISKIAPGCYFLHTK
                     QNEVTAITKIIIQ"
     gene            complement(16746..17603)
                     /locus_tag="PLL28_05670"
     CDS             complement(16746..17603)
                     /locus_tag="PLL28_05670"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HNF68844.1"
                     /translation="MMNRTRINVLMLSVALLPFAFSSCDEVGPTIDLTDTEYETGVGT
                     PETPQNKVVLIEDFTGAACPNCPDAHIAISDAISAHPGRVTAIAEYNYFADPLYIEQN
                     LTTTESYQLDDYMGPVVGWPASFIDRTDFEADGYIPEVPANIASYVNDQLALSSPCNI
                     EITPTFDAATRNLHVLVKVAYTSDVTNVNHLSVALVESGIIAAQIDDNVGGEVEDYVH
                     NHVLRKFLSFYLGEALPEENVAGRWYTFEYDYTLPDTWNADNMAVIGFVHNFETENKT
                     VIQAAEADL"
     gene            complement(17614..19203)
                     /locus_tag="PLL28_05675"
     CDS             complement(17614..19203)
                     /locus_tag="PLL28_05675"
                     /inference="COORDINATES: protein motif:HMM:NF039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HNF68845.1"
                     /translation="MRKSFLVALMIASAMTVFGQGAISGDLQLNTEFYQRDSLIGATG
                     TPHYDNLLSGANSWLTTYYRNDAWGLEAGVRLDMFLNSNLHNPGIPYTDAGIGRWYIS
                     KKIDAFKITGGYIYDQFGTGLTFRAYEERPLGIDNALFGVELEYQINDDWKIKAIGGQ
                     MKDLFTRYEPVIKGATIEGLLPTKNSNITIAPGASFVNRTIDQDNMDIIVAQINSYDL
                     ADRFVPKYNMYAGSIFNTLSIKSVTWYAEYAMKSTDAIRTPSGILDDSKGNVLYSSLT
                     YSTSKIGDGFGITGQVRMVDNWVMRVSPNTTLLDGIMDYLPSLTKQNSLRLTARYQAV
                     AQELGEFAYQVNGTFTPKKGYTININYSNVTNDTTELFTEVFADFEMRKQKYKLLLGG
                     QLVHYNQAIYENEPGRPMVEAITPFAEFTYKFSKKKSMRMELQYQHCEQDFGSWVYGL
                     VEYNIAPKWSFAVSDMWNYDPLKTDDALHYYSVFAGYTQKSTRFTLNYVKQIAGIVCT
                     GGVCRYEPAFSGLKAGITTTF"
BASE COUNT         5938 a         4200 c         3735 g         5334 t
ORIGIN      
        1 aaacaagtga gatgccgaat aataaaacgc cagagctgaa tgctgaagaa ataatgtatt
       61 tcatggcagc ttcaccactc ctgcgcaatt gcagatcaaa gtttgccagt gcagccaatg
      121 gaatggttgc cagttccaaa ctcaaataca ccatgaacat attctgcgca gaaatcataa
      181 agaacatacc tatgagcgat gccagaagaa tgatgtagaa ttccgcggta tgatgatgat
      241 tctttaacca tggccacgac tgcaaagaga taatgagcaa tccgaggttt aaaatacttt
      301 tttggaaaca gatcagtggg ttgctgaaaa acatatcgcc gaataagctg ccatgcgcat
      361 tgcagaaaaa tcctacaaga agattaatcg taagcaaacc attgattaca agcaatagcg
      421 aatcacctgt aatgcgtttc tttcccagac gaagcaatag cactatgaac atgatgactg
      481 ttgccaccag ttcaaattgc attactgaaa aaaagttatc cggcatgaat gatattattt
      541 tagtaattca atttcgtttg taatagatgc acctgatgca attttatcca tcaatacatg
      601 agtgcccgga taaatgatat cggtgataat aaatggtgcc agtcccatga tcaggatgcc
      661 tgtgagcaac acgaatgctg cagtgcgttc gttccattta gcatctgaaa ataaggcgtg
      721 ctcctgattc acaagaggtc ccatcattgt ttttcctgct gcgcgaagga tataaaccgc
      781 tgtaaccaca atactggcac aagctaaaat ggtggctaca cgatataagg tttccggatt
      841 ttgccatcca cccataaata cagtcatttc ggcaacaaat ccactcatgc ctggcaagcc
      901 tagtgaacat aaaccggcca aaataaatac ggtcccgata aatggcgtga tcttcaataa
      961 accaccaatg tctttcacca tccttgtatg cgtacgatcg taaaccatgc cgatagcggc
     1021 gaagaaaagt gccgtcatca aaccatgaga taccatttgc atcactgcgc cgttaatggc
     1081 tgtttcattc agcataccga ttcccaataa cacaaatccg caatgcgata cggatgaata
     1141 ggcattgata tattttaaat cggtttgcat cagcgtggca aatgcaccgt aaataatggc
     1201 gatggtagat aatccgataa taaggggttg gtaataatgt gctgcttccg gcatcaacca
     1261 agtggcagcg cgtagacaac catatccgcc aagtttcatg ctgataccgg cgaggaacat
     1321 tgatgctgct gttggggcgc tgctatgtcc atccggcacc caggtgtgaa atggaaacag
     1381 tgctgtgaaa atgccgaagc cgacaaataa aaacggaaag aaaaacattt gtttgtccgg
     1441 atccatggtt atatgtgctc cggagctgag gtcgaaactt tgtccgccaa agaaataagt
     1501 tcctgccaat cccacaaata ccaatgcgct accacccatt aacatcaaag caagtttcat
     1561 ggctgcatat tctttcttcc cgctgcccca aatgcctatc aacaaaaatt ttgggataac
     1621 agctacttcc aggaagaaaa acaaggtaaa tagatcgagg gcaatgaaaa aaccatatgc
     1681 tccggtgcta agtagtatca gtaaaagaaa atactcttta gaccattttt cctgacgcca
     1741 gctgatcaac acacctgcca gcacaacaaa agctgtaagc atgatcatgg caatggaaat
     1801 accatcaaca ccaatttgat aattgatatt taatgaaggg aaccacttat aatattgtgt
     1861 gaaaacaaaa ggatcctgat tgcctgaagc acgctcggca cggaatgaga aaaataatac
     1921 gaagctcaat atgaattgca ccaacgctcc tgccaatgcc accatgcgaa cacgaggcaa
     1981 gcctttgacc gcgattatac caatggctgt ggcaaccggt attaatatca ataaattcag
     2041 attcatatgt tcatttcact taataataaa atatcgcgaa tgtatttttc tctcaccact
     2101 aaatactatc cggataactt acatccatat atacaggcat atcagggtaa gtccaataac
     2161 acctgcgaaa aaatacatgg cataaccctg tacttttcct gattgcatac ctttaatact
     2221 atcggaaatc tcacccgtca tcaacgctat gaaattcatg gtgccatcca cgatatttct
     2281 gtcgatccat gctgcggggc gaccgataca attaaagatt actttcttag tgataaaaat
     2341 atatagttcg tcgatataaa atttatgata agctgtgcga taaacgccac gcatcattgc
     2401 aactgctttg tctgctctgt cgttttcgtt tttataccac atcatcgcca ctgctattcc
     2461 caataatcca ataagaaccg gtgcaatgct gaacatgata tcagtatgca tatgaccttc
     2521 agtccgcgtt gcagttacat agtgtgcaaa tggtacgaag ccggcagcga tggataatgt
     2581 ggcaagaatc aataaaggaa acttcattga ccaggcacct tcaccatgga aatgatggag
     2641 atgaatatcc gccttattcc agaaaatgcg gaaatacaat ctgaacatat aaaatgcagt
     2701 caacccgctg gtaacaagtg ccacataata gatatcggga tgcgtggtat atgcggcatg
     2761 caaaatacct tccttgctga aaaatccggc aaatggaggc acgccggcaa tggcaagaca
     2821 accaatcagg aaggtgatat gtgtgatagg taaatatttt ctcaatccac ccatgtcttt
     2881 catgtcattg ctatgcacgg catggattac aagtccggca ccgaggaaca acaatgattt
     2941 gaaaaatgca tgtgtgaaca aatgaaacat actagccgta aatcctgcat catgcgcttg
     3001 tgaactcacg ccaagtgcaa acatcatgta gccgatctga ctcatggtgg aatatgccaa
     3061 cacacgtttg atatccgtct gtgtgcaagc gattaaagct gcaaacaggg cagagaaaat
     3121 tccgatatag gcaacaacat caattgctcc cgatccggta atggcgaaaa caggaaataa
     3181 acgcgctaca agatacacac ctgcaacaac catggtagct gcgtgtatca atgcagacac
     3241 cggtgtcgga ccttccatgg catccggcaa ccagatgtgt aatggaaaca tagcagactt
     3301 accggcacca ccaataaata tcagcaccag tgcccaggtt acaacggaaa atccgaggaa
     3361 tgttgctgaa tatcctttca gaaattcagg acttgtaaga ttggcaatga tatctgtaaa
     3421 attgaaagaa tgtgcggtta cacctgtgat gagaataccg attaaaaaga aaagatcggc
     3481 aaagcgcgta acgatgaatg cttttttact cgcagcaaca gcacttggtt tgtcgaaata
     3541 aaatccaatc aggaggaagg aggaaacacc tactaattcc cagaaaatat aaatctggaa
     3601 taagttgctc gacatcacca gaccgagcat agagaaggta aacaattgca aataggcaaa
     3661 aaatgtagca tatctgcttt cacccttcat gtaacctgtt gagtaaatat gtaccattag
     3721 tgaaacaaag gttacaacca cgagcatcat cacagaaata ggatctaata agatgcccat
     3781 attgatagat aaggtatttg tgaattgcaa ccactgcata tcgacaggca ggagctgagg
     3841 ataaacacca tttgctgcac catcgacaat aaaatattga taagcggtaa acaaggataa
     3901 taccgcagat gtgccgatca acaaagtagc gagccatcct gaaagggccg ggaaatattt
     3961 ttttcctgcc agaccgatta aaacaaaacc ggctattggc aggattggaa tgagactgat
     4021 ataatcgtaa ttgctcatgt ttaatacttc atttcttcgc tatcttcaac gtctattgat
     4081 tgatgattgc gataaatatt gataatgatg gcaatggcta cggcagcttc agcagcagca
     4141 attgcaataa tgaatatggt gaaaaaaaca ccgtctaacc tttcaggata gaggtatttg
     4201 ttaaatgata caaagttgat atttacgcta ttgaggataa gttcaacagc catcagcatg
     4261 gtgatcatat tacggcgtgt gaataagccg tatacaccga tgaaaaacaa tgcagtgctg
     4321 ataaataaaa tttgttctat tccaatgttg ttcatgattc ctgaggttta gattttaatg
     4381 caatcacaat acaacctatc atcgctgcca gcaataaaat actgatcact tcgaatggca
     4441 atgcataacc ggtatcctga taatccaaca tttgatcacc tatgcgctgt ggtgaaacat
     4501 taaactgatc gccactgcct ccgataaaac catattggcg aatcagtgaa taagtaagtg
     4561 ctgcaccaag aatcgctgcc agcgccgaaa atatcatccg cgtatttgtc ggacgaggca
     4621 tgtccttacc cgtttgctga gtcaggaata tagaaaagat gatgagcact acgataccgc
     4681 caacatatac gatcacctgc actgcagcca caaattctac atgcatccaa aaatacaacc
     4741 ctgccacacc gatcagcgag aacaataaaa atattgcagc cctgaagatc tttcttgtgg
     4801 tcattgccag cacgccattg ccaataataa atgcggccag taaataaaat atgatgctat
     4861 aagcgttcac tgataacaat tatgtttttt aattcaattg cttttgatag ttatgcatta
     4921 tatcactcaa cgccttccct gattttgctg cccggtttat tgagcacctt ggttagttcg
     4981 gaacgatcga aaacactgtg ctcgaatgaa ttcgacatgg tgatggcgcc ggttgggcaa
     5041 gcatcaacac acaaattaca catcgtgcac agttcaagat gatatacgaa tgtatctatc
     5101 gcttttttct ttttgccttc aggggtgata tcaaatttgg tgacgatttt aatggtgcca
     5161 ttcggacaag caagctcaca agcagtacaa cctgtgcagg catgctcatt gttttcatcg
     5221 tgcgtcatta ccacttcacc cctaaatcgt tcagctaata caggcttttg attaggatat
     5281 tgctgagtga tgatattttt gtgatgcgtg aaataataac ccgttctccg catacccgta
     5341 agcagcgatt tcaccccacc aaaaacctct gatatgtatt tacttaaaaa catctttata
     5401 attcatttca acccaataaa ctcacccacc cgcctgctcg gcgaggtaaa ctcacccact
     5461 gacccacttt ctcctctctc ctctctcctc tctcctttct cctctctcct ctcaaaaata
     5521 ccacccctga atcgccacca atgtcgccaa taacaaatta aacatcgata tcggcaacaa
     5581 atatttccac tccagattca acagctgatc aatgcgcaaa cgcgggaatg tccagcgaaa
     5641 ccacatgatc acgaaaatca ggaaaaaggt ttttccaagg aaccataccg acgaagggat
     5701 ataatccatt atatgattaa atgcctccca atttccgata tgcaatggca tccagcctcc
     5761 caaatacaag gtggcgccaa tggcacatac aataaatata tttacatatt cagcaaggaa
     5821 aaacaatgcg aatttcattc ctgaatattc tgtatgaaaa cctgccgtta attcagattc
     5881 agcttccgcc aggtcgaatg gcgctctgtt agtttctgca gttacggcaa taataaaaat
     5941 cacaaaagaa ataatcaccg gaatatgtcc cttaaaaatc caccagccat cctgttgcga
     6001 attaatgatg ctgctgatcg acatgtcttg cgagaatatt acgatggtaa gaattgataa
     6061 tcctgcgctt aattcatagc tcacaatttg tgctccgctg cgcatagccc caagcagtga
     6121 atacttgtta ttactcgccc atcccgccat caaaatacca ataacaccta ttgatgaaac
     6181 ggcactcaca tacagtacac cgatattaac atcccaaatc acaaagtctt tcgcaaaagc
     6241 aatcggcgcc aacaccaaca tggaggctat catcacaaca aatggtgcaa gattgaataa
     6301 aaacttatca gctccggcag gtgtcaaacc ttctttaacc aataatttca aggtgtcggc
     6361 taccgtttga aacatgccct tcggtccaac gcgattcggc cccagacgca gctgcatata
     6421 tgccgatact ttcctttcca tcatgatcaa caccaaacct aaaactgcaa acaggccgat
     6481 acacaaaaca ccaacaatca caaattcaat cgtaagtgcc agccaacttg cgcaatgggc
     6541 atttaaccac tgatctatac tgtctgcaat cgatgctaaa ctaaattcca cgttaataat
     6601 ttatactgtt cgtgtcgtgc actatttttt ccggatgttt attccggagg gtaatttaaa
     6661 atgtcattta gcctgaacct gcgctcaagc tgtttatctg tcaatatccg gtattaccag
     6721 atctaatgtt cccataattg ccaccagatc accaattttt tgccccttcg ccatatgatc
     6781 taatgccgaa agattcgaaa atcctggtga acggaatttt attctataag gtgttaaacc
     6841 tccttcgcta acgatgtata tacccaattc tccacgagct gtttccacgc gtgtgaaaaa
     6901 ttctcccttc ggtaatttta atacagcttt tgttttcgct acgaaatcgc cttcaggaat
     6961 attgtcaatt aattgttcga taatactgat cgactgcctc atttcctcca tcctgacaag
     7021 gtaccttccc cagcaatctc cggcagtttc tattgcttct gagaattgta attgtgcata
     7081 gatatcgtaa gggtattttt ttctgatatc gcatgaaaca ccactggcac gggcagttgg
     7141 gccgctacat ccataggaaa tggcgtcttc ttttgatata taacccacat ttttcatgcg
     7201 gttctggaag atgatattac cggtaaccag ttcatcatat tccggtagtt tggatttaaa
     7261 aagggtgaga aaatccttta cttttttctg gaaatcagga tgtaaatcaa acattacacc
     7321 tccgggaacc atataattca ttgtcaatcg tgcaccgcaa gtttcctcca tgatgtcgtt
     7381 gatcaattcg cgttctctga atgcatggaa aaatggtgtg atggcaccca catccattgc
     7441 cagtgctccc caccataatt gatgtgaggc aagcctggta agctcgtcca ttaaaactcg
     7501 tataacctgc gcacgctgtg gaacttcaat ctgtaagcct ttttcaactg ccaaggcaca
     7561 tccatggttg ttgatatgag ccgacaaata atccatcctt gatgtagaat aaataaactg
     7621 gcgataactc aggctctcgc acattttttc gatggagcga tggatatagc ccaaatgtgg
     7681 ttccaccttt ttaatggttt cgccattcaa tgtgatcacc aggtgcaata caccatgtgt
     7741 tgaagggtgt tgaggcccta catttatcac caggtcgccg gtatctgttt ttgctacttc
     7801 ttgtaacatt tacgctttat aatttcgtcc cttgcaggga tttcattcct tcccacaaca
     7861 atgaataatg ctcgttatta gagcttaatc atattgatag gatcttcata atctttgcgc
     7921 atcggccatc cttcccattc gtcagtaagc aatagccttc tgagatcagg gtgatgaata
     7981 aaccttacgc caaataaatc aaatacctcg cgctccaaca attctgcnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnntct tcgaaggtaa cctgtggctc caggttggtg
     8161 atggcggctt gtagttcttc gcgtgtcatg ctctgctgat tcgtttatta ttgattgtcg
     8221 taaaatttct ctatgacgat tttttcacgt ttgcctcccg cttgaatctt tgtctgcaag
     8281 gtaatcaagg catgcagaag ggcctccgga cgcggcggac aaccggcaac atacacatct
     8341 accggaatca cgtggtcggc acccttaaca acactatatg tattataaaa gaatggtcct
     8401 ccgctggttg cacaagctcc catggcaatc acatatttgg gatccgccat ctgatcgtat
     8461 aagcgcttaa gcacgggggc catcttattt acaatggttc ctgcaataat tattacatcg
     8521 gcttgtctgg gtgttgctct tgctacttca aacccaaaac gactccagtc gtacttggca
     8581 ctggcggtgc tcatcatttc aatggcacag caactggtgc cgaataccaa tggccaaaga
     8641 gagttacttc ttgcccagtt aatcacatcg tcgaggcgac tcacgagtaa acctcctgtg
     8701 gctgtagggt gtacatcgcc ggggaaaggc tgttgttgat atcctggctc ttttacatcc
     8761 atttcaaagc acctttttta tgggcatata aaaagcccat gaataatata aaaaggaata
     8821 ttaccgcttc ggtgaaagca aacatcccca accgctgaac agacactgcc cagggataaa
     8881 gaaatatgag ttccacatcg aaaatcaaaa agataagggc gaaaaggtaa tatcctacgt
     8941 tgaactgaac ccagttttgc cctatggtag gcacaccgca ttcgtaaggt tcgcctttta
     9001 cggggttttt gctcgctttg agcaccactt tggaaaggag gatacccgca gtagagaagg
     9061 ctaccgctgc catgatcaat actaataatg aggccggacc cataggtggg gctaaaatac
     9121 gccttgatag gtaaaacgac aaatgtggtt gtgcaccttc gtgggttttc aaccaaattc
     9181 ccgtaacgca agccttaaaa accttaaacg aaaaaaaatg tttagcggat caacaggtat
     9241 tacatttcaa gcacccctat tattgaggtt gacatttatg gtaactattt gccatttact
     9301 gctaaaacaa gttcgaccaa tctaaatgca ggaatggtgg taaacagcaa gtaaataaaa
     9361 taatattcag atgaataaaa gcaggttccc ataccataat caaactatat attgatatta
     9421 taaaaaaaat caaaaaataa acatcaaaac cacctttaac ttttttcaat ttcatgatat
     9481 aattccaaaa aaaatccaca ggaaatattt aacatttttt gaaagttgac ttaaatcacc
     9541 aatcttgctg agcaaaatca tgctgttccg tatgatataa tgagaagttt acgtctgatt
     9601 gcgggttcac ttattactcg tacttaaatt ttcagacata tgtcacatac agaatctcaa
     9661 ctgatctccc ccgatgtaaa acacggaaat tttaatttca aacgcgatat catggatggc
     9721 aacgaagcag ccgctaccat tgccttcaag accaatgaag tctgcgcaat ctatccgata
     9781 acaccgtctt cgccaatggg tgagtgggcc gatgaatggt cttcaaaagg tgtgatcaat
     9841 attttcggtc aagtgccaac agttatcgaa atgcaaagcg aagcaggggc agccggtgcg
     9901 attcacggag cgttggccgc cggagcactc agcaccacat ttacttgttc gcaaggatta
     9961 ctgctcatga tccctaacat gttcaaaatt gccggtgaac ttactccagc cgttttccat
    10021 atcgccgcaa gagcagtagc aacgcatgca ttatcgattt ttggcgacca tagcgatgtt
    10081 atggcggcca gagcaacggg tttttccatg ttgttcggtt gcaactcaca agaggttatg
    10141 gatatggcat tgatttcgca atctgcaaca ttatctgccc gcgtgccttt catgaatatt
    10201 ttcgacggct ttcgcacaag ccatgaattg agtgaagtag aaattattcc tgatgagatc
    10261 attcgtgcca tgattaaaat ggaagatgta atggctcatc gcagcagggc tatgaatccg
    10321 gcaaatcctt ttattaaagg aacggctcaa aatcctgatg tgtttttcca aggtcgagaa
    10381 gcttccaata aatattatat gcaggtgcct gaagtagtta aggcgaaaat gaaagccttt
    10441 gcatctctta caggtcgcac ttataacttg ttcgattact atggtcaccc tgaagctgaa
    10501 agcattatca ttattatggg ctccggagca ggtcctgtgc aggaaatcgt cgattatctc
    10561 aatgcaaacg gtggaaaaac cggcgttgtg atcgtgcgct tatatcgtcc atttgctata
    10621 gacgattttg tgcatgcaat cccttcaacc gtcacaaaaa ttgctgtcct cgatcgttgt
    10681 aaagagcccg gtgccattgg cgaaccattg tatatggatg taatcaatgc gttgcgcgaa
    10741 aaggaacgcg atattagaaa agtcattggc ggacgctatg gtctgggttc taaagaattc
    10801 aatgcctcta tggcgaaagc ggtttttgat gaattaaatc aaccaaaacc taaaaatcat
    10861 ttcactatag gtattgacga tgatgttacc aataccagct tgcacttcga tcctgatttc
    10921 aacctcgaaa aacaatatta cttccgcgga ttgttctatg gtcttggtgc cgatggtacc
    10981 gtgagtgcaa ataaaaattc catcaagatc attggtgatc ataccggcga ttttgttcag
    11041 ggatattttg tatatgattc caaaaaatca ggctcgctaa cagtatcaca tctgcgcgcc
    11101 ggaaaaaatc ctattcgctc gagctacctg gtgcaatctg caaattttgt ggcctgccat
    11161 cattttaatt atctgctgaa atatgatgtg ttacgctatg ccgaacatgg cgccgttttc
    11221 ctgttgaata caccataccc tcatgaggaa gtatggcaac atcttcccaa aaaaattcag
    11281 gaagaaatta ttgaaaaaca attgaaattc tttgtgataa atgcaagtgc agtcgcacga
    11341 caagcagaat tgggcacacg cattaatacc attctgcaaa cctgcttctt tgccatcagt
    11401 aatgtaatga gcagcgatga ggctatcgca gatattaaac aggcaattca cgattcctat
    11461 attaaaaaag gtatctcagt agtggagaaa aattatgctg cggtagataa ggcgctcgca
    11521 aatttgtttg aagtcgacta tacaaaaaat gcaaccggaa atctggaagg agataaaatt
    11581 gttcctgatg atgctcccga cttcgttcaa aaagttctcg ctcgaatcat ggctggtgaa
    11641 ggcgatgaat tacctgttag tgcttttcct gtagacggta gatatcccgc aggaacaaca
    11701 caatatgaaa aaagaaatat cgccgagcaa gttccggtat gggacgcaga cctctgctca
    11761 caatgcggaa aatgttttat gatttgtccg catgcagcca tcagaccaaa agtgtatgct
    11821 aaagaagcac ttgctgatgc accgccaact ttccgacatg tagcaccaat tggtaaagaa
    11881 tttaacaagg aggaagaagc atataccttg caagtaagtg ttgaagactg caccggatgt
    11941 aatttgtgcg ttgcctattg ccctgttgaa agcaaacttg aaaaaggaca caaggctatt
    12001 aatatggttg acaaacttgg tcttgaagaa agtgaacagg ccaattggga tttcttcctc
    12061 tcacttccag acatagatcg agaaaaagta aataaaaata cagtcaaagg cactcaattc
    12121 ttgcaaccat tattcgaatt ttcaggtgct tgtgctggtt gcggcgaaac accatatttg
    12181 aaattgctga ctcaattatt cggcgataga atggtagtgg caaatgcaac cggttgttct
    12241 tcaattttcg gcggtaactt gccaacaaca ccttggacta ccaatcatga aggtcaagga
    12301 ccggcttggg caaattcgtt gtttgaagac aatgccgaat tcggattggg tataaagttg
    12361 gctacagata aaaaacgtga aatagcctta tacctcctcg accaaatgca accaatgatt
    12421 gatgcaggat tatatgatgc cataaaatct catcacggca ctgcagaatc tgatatcaat
    12481 atatataaac aacaaatcgc agcgctaaga aaacaaattc gtggtatcga taatccaaat
    12541 gcacatatgt tgctgcattt cgcagattac ctgattgaaa aatctatttg gataattggt
    12601 ggcgacggct gggcttatga tattggtttt agtggattag atcatgtgtt gtctaccggc
    12661 gaaaacgtga atattttcgt gttagataca gaagtgtatt ccaacaccgg cggtcaaaaa
    12721 tctaaggcat caccgttagg tgcaagtgcg aaattttcta tcaatggtaa acgcagcggt
    12781 aagaaagacc tagcattgca agcgattgct cacggaaaag catatgttgc tcaaattgca
    12841 atggcggcaa acgatatgca taccgtgaga actattcagg aagcagaagc attcccgggt
    12901 cctagtttaa taattgcgta cagtcattgt atcgctcacg gctatgacat gtgccatggc
    12961 agcgatcaac aaaaaaacgc tgtaaattcc ggatattggc cattgttcag atataatccg
    13021 ctcaaagcaa gaggcgaacg atttacaatg gactccaaag acccaagtat cccactcagc
    13081 gaattcctct atcatgaaaa tagattcgcg tcaataagag cgaaagatcc cgccctcgca
    13141 gaaacattcc tgcacatggc agaagcggat ttgaaagcca aatatgaacg attgcatcta
    13201 ttggaatcga tgtgatcgat tgtcattatt aatggaatac cttgaaagga gggacgacag
    13261 ttttattcgt ccttcctttt ttaattacaa taagttctga tacctatccc gcacctcact
    13321 catacagttg ccgaattacc caattacagc tagactgact tctattaata agcagttatc
    13381 aaaactatta tttagtaaat aatttaagtc atattgataa attgttaaca taattgcgca
    13441 ttgttaatca tacatatagc aaatatgtcc ttaaactaaa taaaacaata cgttaacaat
    13501 gagttaacac tactctcggt aggtaactct acctttgctc aaaacctatt tgcatgtcaa
    13561 ctttaaaaaa gactttcttc gccttgacac tgctttttgc aacgcagcag ttcgtacagg
    13621 cacaaacaac cgaacaggct ttcagccctt ctatcaaggt gaaagcattg attcactctc
    13681 ggtttgaggc ttctcttacc gatagcatcg atgtagcagg taaatatttt gctaaccctg
    13741 taacaactaa ttttcgcatg cgtcgcattg agctgcgcac agacattaaa ctgaatgata
    13801 aattttcagg agttgtacgt gttcaattac ccgaattgaa aggctccagt gtgggtaaag
    13861 tgatcgagtt ggcatatatg gattataagt ataatgatat gctaaatgtg cgtttcggtc
    13921 aattcaaatc tcccttcgaa cttgatgaac aaaccagtca cgaagacctt cgcatgatcg
    13981 atcgcggacc aacaagtgtt atgttcgtaa ataatagtct cgcatcatac caaccgggcg
    14041 ccatggtatt tggaacattc atgaaatcca aaacaccgct ctcatactat ctcgcttttg
    14101 ttaatggaag taatcgcgca gtaaataatg atgataatag ccagaaaaat attgttggac
    14161 gacttgaatt cgtgccgatc aaaggagtta aaatcggtgc taatgctcag cagatctata
    14221 caaaggacac cagtggtctt tcatatggcg gagatcttag catcattcga gacctaaacg
    14281 ataaaatgaa attaattgtt gaaggtgaat atatcacagg atatcaggta aatgatttta
    14341 atgctgatac aaccggtctg gaaattggcg attatacaat gggtggttat ttcggacaag
    14401 tattattgcg ctctgaatta aataaatcat ggtgtaaaac tttcgaagtg ggtggtaaat
    14461 acgaacatac tgatccaaat accactgttg atgacaatgc atttaatacg ataacaggtg
    14521 gaattggatt cattttcact cctgataata tggctcgctt gcaattgaat atcgtacata
    14581 cagactacga aaaaacaatt actccgggtg gacttatggc tgcaaatatg tttgttacgc
    14641 agttccaact taagttctaa ggcaattaca ttattcaaaa aaagcctgtt tcttccttga
    14701 aacaggtttt tttcgttggt ggttaaacca atcgcacacc cgtctgtcta aatacacact
    14761 taattacctt taccatctat gaacttgaaa tattacatcg ttttgcttat cgcaaacagt
    14821 cttcaactat atgcctttgc acaatctatg cccgaccaaa tggttatttc tgatgatggc
    14881 accagactgt ctgtcggagg tcaggaacca aatgattttt acaatatcga ttcaatcagg
    14941 tcaattaacc tgatctttga cgaacctaat tactggagcg cattaactgc caattatgcc
    15001 tctaaaacag acctggcagc caccatggtt tacaatggtg ataccctggc tgaacaagtt
    15061 ggtgttcgtt tcaaaggtca aaccagttat atgatgaata tgactgataa aaaatcattt
    15121 aatataacca ttgactatat cgatagctcc caggatatcc atgggtataa taccttaaat
    15181 ctcaattgtg gatatgaaga tccttcattc atgcgcgaat cggtttatga aaataatatc
    15241 aggaagtata ttcccggcct tgctgtaaat tatgtgcatc tatatctcaa tggcgaagat
    15301 tggggactct acctcaatgt tcaacagctt aataaagatt attataaaga atggttcatg
    15361 accaacgatg gcacaagttg gcgcgctgaa aaagaaggat tcggcggacc gggtggcggt
    15421 gatcccttcg gcgcaggata ttgctctctc aactatcttg gaactgattc ttccgattat
    15481 acaccatact accaattaaa atcaacagat cttgaagatc cgtggacaga cctgatcaat
    15541 actaccgatg ttctcaacaa cactgctgat gcctctttgg aagacgctgt gaataatgtg
    15601 cttgaccttg accgcactct gtggttcctg gcactcgaaa ttatattcag cgatgatgat
    15661 agttatgtca ataaaggtgg gatggattac tatgtgtatt gggaaaaaga aacagatcgc
    15721 atagtgccaa tggaatatga tggcaatagc tgtatggatc ccatgcattt atcgtggaca
    15781 ccattttatc atgaaacaga tgctgattat gcattattga acgtgctcct cggtaacgat
    15841 aatattcgtc agcgatatct ggctcacatg cgtaccatcc tcgacgaatt attcaactcc
    15901 gattacatgg atgcctacat cgatgcaatg gatacacgca ttgacgcact cgtaaatgcc
    15961 gatcctaaaa aaatctatac ctatgcacaa tacaatgctg atgtcactga actgaaaaca
    16021 tttgtcgagg aacattataa tgatttcatc aataataccg aaatagatcg ccttgaccct
    16081 gaaatttcag aaacactgat gtattccgca gcaggagatt ggattgctcc cttatcaatg
    16141 gaagcacccg tagtaaccac aaatgtgtat actgaaagca atatcaatgg tgtaaatctc
    16201 tactacggca cagggctagt tggaagattt tacccgctgc aaatgtttga cgatggcgct
    16261 cataatgatg gactttttaa cgatggaacc tggggtgcaa atctccccgc tatgcctgca
    16321 gggactgatg ttcgatacta cattgaggca attgccgatg atggatacgg aacacgctcc
    16381 tatgaccctg taggcgctga acacgatgta tattattatc aagttcgagg caatgtggcc
    16441 gtagaagact ttattgcaga aactattaat gtttatccga accctgccac tgatatggtg
    16501 caaattattt tgccgtcatc aaatgaaaca gctgatatcg aattgttctc tcttggagga
    16561 aatagtgtgc tgaatcaatt aaattataag tcaggagcac ctctcaatat atctaaaatt
    16621 gctccgggtt gttatttcct gcatacaaaa caaaatgaag ttacagcaat aacaaaaatc
    16681 ataattcagt aaaaaagaaa aagccatccg aaactcgaat ggcttatttt ataagtgtta
    16741 tctgtttaca aatctgcttc agcggcttgg attaccgttt tgttttctgt ttcgaaatta
    16801 tgcacaaatc ctatcactgc catattatcg gcattccatg tgtcaggaag cgtgtagtcg
    16861 tattcgaatg tataccatct gccggcaaca ttttcttccg gcaaagcctc accaaggtaa
    16921 aaacttagga attttctcaa tacatgattg tgtacgtaat cctcaacctc accgccaaca
    16981 ttatcatcga tctgcgctgc aataatgcca ctttcaacta atgcaacact taaatgattt
    17041 acattcgtaa catcagaagt ataggctacc ttaaccagca catgtaagtt gcgtgttgcg
    17101 gcatcaaacg ttggggttat ttcaatatta catggagagc taagagctaa ttgatcattc
    17161 acatacgaag caatattcgc aggcacttca ggaatatatc catcggcttc aaaatctgtt
    17221 ctgtcaataa atgaggcagg ccatcctaca accggaccca tgtaatcatc aagttgatat
    17281 gattctgtag tggtcagatt ttgctcgata tatagtggat ctgcaaaata attatactct
    17341 gctattgctg tcacccttcc aggatgtgcg cttattgcat cactaatagc aatatgtgcg
    17401 tcggggcaat tcggacatgc agctcctgta aaatcttcta ttaacactac tttgttttga
    17461 ggcgtttcag gagtgccaac accggtttca tattctgtat ctgtaaggtc aattgtaggt
    17521 cccacctcgt cgcacgacga aaatgcaaaa ggcaataaag ctaccgaaag cattaatacg
    17581 tttatcctcg ttctgttcat catacaaata aatttagaat gttgttgtaa tacctgcttt
    17641 taatccgctg aaggctggct cgtaacgaca aacacctccg gtgcaaacaa tacccgcaat
    17701 ttgtttcacg taattcaacg tgaatcgggt tgatttttgt gtgtatcctg caaaaacaga
    17761 ataataatgt aatgcatcat ctgttttcaa tggatcgtaa ttccacatgt cgcttacggc
    17821 aaaactccat ttgggggcaa tattatattc aactaatcca tacacccagc taccaaagtc
    17881 ttgttcgcaa tgctgatatt gcaattccat gcgcatcgat tttttctttg agaatttata
    17941 agtaaattct gcaaatggtg taatcgcctc taccattgga cgcccgggct cgttctcata
    18001 aattgcctga ttgtagtgaa ctaattgtcc tcctaataat aacttgtatt tctgcttgcg
    18061 catttcgaaa tctgcaaata cctccgtaaa taattcggta gtatcgttgg taacattcga
    18121 ataattgatg ttgatggtat atcctttttt cggagtgaaa gtgccattca cctgataagc
    18181 aaattctccc aactcctgcg ccaccgcctg gtatcgtgcc gtaaggcgaa gtgaattctg
    18241 cttcgtcaat gaaggcaaat aatccattat cccatccagt agcgtagtgt tcggagatac
    18301 tcgcattacc caattatcaa ccatgcgcac ttgcccggta ataccaaaac cgtcaccgat
    18361 ttttgaagtc gagtaggtga gtgacgagta taacacattc cccttgctgt catcaagaat
    18421 cccacttggt gttctgatag catcagtcga tttcatcgca tattctgcat accaggtaac
    18481 actctttata gataatgtgt tgaaaatgct ccccgcatac atattgtatt tcggaacaaa
    18541 cctgtcggca agatcataac tgttgatttg cgcaacaatg atatccatat tatcctgatc
    18601 tatcgttcgg ttaacaaaac ttgctcccgg cgctatcgtt atattcgaat tttttgtggg
    18661 caaaagacct tcaatcgttg cacctttaat aacaggctca tatctggtaa acaaatcttt
    18721 catttgtccg ccaattgcct ttattttcca atcatcattt atctgatatt caagttcaac
    18781 gccaaaaagt gcattgtcta tgcctaatgg tctttcttcg taagcacgaa atgtcaatcc
    18841 ggttccaaat tgatcataaa tatatcctcc cgttatctta aatgcatcga tctttttact
    18901 gatataccac ctgccaatac cggcatctgt atatggaatc ccgggattat gaagattcga
    18961 attcaaaaac atatccaggc gaacgcctgc ctccaatccc caggcatcat tcctgtaata
    19021 ggtagtaagc cagctattgg caccactcaa taaattgtcg tagtggggtg taccggtagc
    19081 cccaattaga ctgtcgcgct gatagaattc cgtattcaat tgcaaatcgc cggatatcgc
    19141 tccctgtcca aatacagtca tcgcagaggc aatcatcaag gctactaaaa aactttttct
    19201 cataggctaa ggtttccgac gctaagttaa gcacctgtgc cgactttgat ctccctcctt
    19261 ttcaaattga caggctcctg tagtgtaaaa tgctggatac cggtaat
//
LOCUS       DAOQHY010000048        18871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1329_length_18871_cov_4.856027, whole genome shotgun sequence.
ACCESSION   DAOQHY010000048 DAOQHY010000000
VERSION     DAOQHY010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 18871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18871
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1329_length_18871_cov_4.856027"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            411..836
                     /gene="tsaE"
                     /locus_tag="PLL28_05680"
     CDS             411..836
                     /gene="tsaE"
                     /locus_tag="PLL28_05680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNF68846.1"
                     /translation="MKPRIFTLDETTQIAKMLNAEAKHSHIFLFHGEMGSGKTTLIKS
                     MCRQLGVNEHTSSPTFAIVNTYQGTQHIIYHFDLFRLRDVHELEAIGFSEYVDSGNML
                     FIEWPEQALPLLKGYEVVKIVLETIDTEHRRISMQIETL"
     gene            884..2113
                     /locus_tag="PLL28_05685"
     CDS             884..2113
                     /locus_tag="PLL28_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNF68847.1"
                     /translation="MSDPTQKSILTELAAAAGLQTLEKYADVDQKKQRLFIGIPRETG
                     FQENRVPLTPDSVETLVAQGHRIVIETKAGEGARFSDKDYADAGAMVMYDRKQVFEAD
                     IILKVTPPAMDEMELFRHGQVLISPLHLPTLRREIIHDLMKKQVTALAFEYVKDESGT
                     YAFVRCMSEIAGASAILLASELLADAIHGRGYLLGGLSGIPPTQVLILGAGVVGEFAT
                     RAALGLGATVKIYDNNVYKLSRLQNNIGERFYTSVINNRRLLNDIRTADVVVGAIHAE
                     SGRTPVIVTESMVMEMKPGSVIIDVSIDQGGCIETSEVTTHLQPTFIKHDVIHYCVPN
                     IASRVPRTASYAISNIASSTLQQFTEYGGLDPLIRNLEGIRHGVYIYKGHLTNYYLAQ
                     KMEIKYTDINLLLPSVL"
     gene            complement(2196..4286)
                     /locus_tag="PLL28_05690"
     CDS             complement(2196..4286)
                     /locus_tag="PLL28_05690"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HNF68848.1"
                     /translation="MTVKLIRLLFVCCTVLFARPMQAQQTTTCGTDDVRRQLIEQYPE
                     IIQRERAFNIYAQKFDLSQLKVEEGTYIIPVVFHVIHDYGVENISDEQIFDAMEKINE
                     DFNKENSDLAETVTEFLGIAADVQVEFRLAQRELSGACTNGIDRIPSMRTYTGGDAAK
                     LGGWQHGFYLNIWVVKFLAGSAAAYAYYPTSIFDALAPVDGVICRYDYVGSIGAAGEY
                     SSHTLSHEIGHYLNLQHTWGNTNNPGVECGDDQVDDTPVTKGWLSCNLAGHTCIEPLD
                     NVQNFMEYSYCSTMFTEGQKTRMYAALNSTDAQRNHLWSSDNLMLTGTNDGYVAGLCS
                     PEADFYTSQRFVCKDESVTFHDASWRGDVDSRMWTFEGGSPLTSTEMDPVVSFNTPGW
                     HSVTLQVTNASGTTTKTVEQYIYVSDDVASYNADYVGNFNNMDAALNDWVLYNRYPDN
                     YQWNWRGQNGYWNTPCIWLNSRFGPDQEKDEVISPSFDLSDGLTDNVFFKYSTTSNGF
                     DADDYTMKLKLYYSTNCAKTWISVGEITGDDLITSYGGSSNFFPVFADQWRVAAFSIP
                     SGAKAENVRFKIEFVYNNYVNNVFVDDFNFTDGTLGIEGFGGNGTLAVTPNITGVQDV
                     VSLHYTAETAGIVQVQVSDITGRVITTQQTAVTSTGPQTIQLNLADLHLSAGCYFVTL
                     HAHGANATARLVLQ"
     gene            complement(4338..4886)
                     /locus_tag="PLL28_05695"
     CDS             complement(4338..4886)
                     /locus_tag="PLL28_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68849.1"
                     /translation="MKKIVAYIGCITLMATLLINSNGFTLWLSAATYHAKQEVWNRIE
                     AGEVNDIATTLNIPLDHVEHALNGFSWRDDKHEFTYEGFLYDIIQMDMASDGYHIIAV
                     RDDKEKDLEEKIASQLQHQNNTDQKHQSLPKLQLDTQFDVVALTAYFVPDQTLIMQTN
                     VSKFIESSGYLNSQINPPDELI"
     gene            5232..6434
                     /gene="dinB"
                     /locus_tag="PLL28_05700"
     CDS             5232..6434
                     /gene="dinB"
                     /locus_tag="PLL28_05700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNF68850.1"
                     /translation="MRLSMDHSGRMIVHIDLDTFFVSVERLKDDRLKGKPVAVGGTGD
                     RGVVASCSYEARAFGVHSAMPMRQARILCPELVTVRGDYEHYSHYSNTITEIVKENVP
                     LYEKSSIDEFYIDMTGMDRFFGTYTYMQELRERIIRETGLPISFGLSENKTVSKVATG
                     QAKPNNHMKIDHGVEKSFLAPLPVKKIPMVGEVTGQLLRKMGIVQIKTIQEMPQQVME
                     NILGENGRVIWRKANGIDTSPVEPYTERKSISSEETFESDTIDVSRIRTILMAMTENL
                     AYQLRKEGKLTSVVAVKIRYSDFDTHTKQIRIPYTSTDHTLMRVVKELFDKLYERRVL
                     IRLVGVRLSGLVNGGYQINLLEDAEHTIRLYQEMDHVRKRFGDTVVQRASAMGYKMRR
                     FNPFKGER"
     gene            6787..9753
                     /gene="dnaE"
                     /locus_tag="PLL28_05705"
     CDS             6787..9753
                     /gene="dnaE"
                     /locus_tag="PLL28_05705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNF68851.1"
                     /translation="MFLNTHSYYSLRYGVLSPEGLLAEAQRCGVHAFAMTDINNCSGW
                     FELYAEAKKFNIHVVPGVEFKNGDDHLYIGIAKNEHGFAELNRYLSHYLNTKEPIPAI
                     APEFEHAIIIYPFVADKIYVLGDNTFVGIQKKDLSKLRVGKSGLPQEKLVAMLPVTFS
                     SKKDFSAHRILRAIDHNTIITKIPENATALPDQVFRPYDRLYEDFGDHGYLLENAREL
                     LENCTFDFTYHTNKNIRSFSGNEADDINQLLQLTYEGVKRRYGKLTKTIQDRINKELE
                     IIIQKGFVAYFLINWDIIRYAHSKSYFTIGRGSGANSLIAYCMGITDVDPIELDLYFE
                     RFINLFRETPPDFDLDFSWKDREDVTDYIFKRYGEEHTVLLATYTTFKSRSFIREVAK
                     TFGLPKYEIDAMIKNYGESIFGDSIQIPDKIARSIYNFGAYLQGNPNYLGIHAGGILI
                     SELPVYYYTATHLPPKGFPTTQFDMHVAEDIGLYKFDILSQRGLGHIKETVEIVKQNR
                     GDDIDITRVQDFKKDPVLNNNLVKGNTIGCFYIESPAMRSLLNKLLCDNYLTLVAASS
                     IIRPGVAQSGMMDEYIRRHRFPEERKRIHPVMGDIMPDTYGVMVYQEDVIKVAHYFAK
                     LTLSEADVLRRGMSGKSRSKDEFQRIKTKYFENCAQLGYDKALANDVWHQIESFAGYS
                     FAKGHSASYAVESYQSLHLKSYYPLEFMVAVINNFGGYYSTELYFHEARKSGATIEAP
                     CINNSRYNTCIYGTVIYVGFVHLSGLESALAEQMLAERAHNGPFTSFDNFLDRVAINL
                     EQLELLITINAFRFTGITKRELLIEAYTRMNKTKKTIPAATLFEKSAPPKYTFPPLHF
                     DAREDAWDEIKLLGFPLSSPFLLISDPIIENRNLKLVKDLPKFVGKTVEIIGYLITTK
                     RTSTKHGEPMYLGTFIDMEGNWLDTAHFPAVAKQYRFKGKACYLLKGKVQESFQAHTL
                     IVSEMYHLKMWVEGEN"
     gene            complement(10142..11185)
                     /locus_tag="PLL28_05710"
     CDS             complement(10142..11185)
                     /locus_tag="PLL28_05710"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNF68852.1"
                     /translation="MKSNVYIFNLNLDWELIKLISQIDRFDAAWTAIERREQQGLKEL
                     KSIATVRSVGASTRIEGSKLSDEEVDVLLRNIDISKLEDRDTQEVVGYFEALDIISEQ
                     FEEIKITESDIKNLHNILMKYSAKDEWHKGNYKQHSNAVEARYTDGTRQILFQTTEPG
                     VETETAMRLLVNWYNNEHAIHPLVKCAVFTYEFLSIHPFQDGNGRLSRLLSSLLLLKS
                     GYKWIQYISFEHEIESRKHAYYQVLRSCQTLRPNIDISTWVNFFFDALKNIQELLERK
                     LLIYETETDQLLPREKLVVSFIQNHPGCKSGQISKGLGFSRMIVIRLLSSLLEKKLIE
                     KHGTGSGTQYTIM"
     gene            11441..11971
                     /locus_tag="PLL28_05715"
     CDS             11441..11971
                     /locus_tag="PLL28_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68853.1"
                     /translation="MKPKLTLLLLIPLLAMSFLIKNDPQTNEINLNTLDQQLIGEWEY
                     KISFSDSSYFTVPFNRILPVQKLRFEHCHDSSELERFPKIVIAMRKHNSLENISCKAY
                     NENNEVIDTYYPVVLFGNDSLVNQIDVSQYGHILKSEYTIYTLTSDSLIIGDGKLFES
                     PDRKMVLVRHIYLRKN"
     gene            12155..12469
                     /locus_tag="PLL28_05720"
     CDS             12155..12469
                     /locus_tag="PLL28_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68854.1"
                     /translation="MKYDIERRANPSNIEVYDSIYLKNGNYCFWYAYNLGGIYGGIGD
                     YISIAPQRSQISDSTQVIVSEYIKGIRAISENEIVVILTEDEYDEIHTTFKGIKISAK
                     ID"
     gene            complement(12594..13406)
                     /locus_tag="PLL28_05725"
     CDS             complement(12594..13406)
                     /locus_tag="PLL28_05725"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNF68855.1"
                     /translation="MVKPQVNAASVTTKRKEIAIFKVPIFRAFLQLIDGVDYICDMCS
                     SYTTKSKMVKEVYEQSFGRPWAEEDLGIEEKYHDESGNNAIAFPGTPMPVVTIERPQI
                     IQAYQFGFIPHWTAPEKVNEQKATFNARIETITELATWRDAWKHGQRCLVCTNGFFEH
                     NKAEKRKMFIHLQDAENFFYAGLYNNYINKKTGEIIRTMAVITTTPNSLIASIHNRMP
                     VIIPIGTESPWLDPNADLKSLLANYTTPFSPAHMLMEYADKKPQKPVQGELF"
     gene            13399..14241
                     /locus_tag="PLL28_05730"
     CDS             13399..14241
                     /locus_tag="PLL28_05730"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNF68856.1"
                     /translation="MTIGGAGCTMDSDTTTQNIPQPEHVVIVMFENKDFAQVYDTSIA
                     PFLQTLIYDPHTAVFTNSLAMGHPSQPNYIWWFSGDDQGVTTNDRPATKFNTPNLAAE
                     LIQNGFSFVTYSENLPFVGFDGDSSENGLYVRRHNPVSNWIGLDVNQVSPDVSQPFSA
                     FPANFDHLPTVSFVVPNMINGMHNGTITEGDTWLQNSLSEYIEYCRNNPSLLVVTFDE
                     TTYNVTDNRILTLCFGSLVNGGEYDAAVSHVNFLRTFEDMFSLPHAGLSGYLEPISNC
                     WVQE"
     gene            14244..15149
                     /locus_tag="PLL28_05735"
     CDS             14244..15149
                     /locus_tag="PLL28_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF68857.1"
                     /translation="MRCKKLFIGLVTLTLSWFPSTVFSQDTIRAHIYFKSGSYETKPH
                     NTYLQRFADACAGFDSAHIFIDGFCDDEGSDAFNIQLSEQRAMAVEAFLKQYITIKAN
                     FTITGHGESNPVASNEFDDTKFQNRRVDMLVIGYQYGYTSSISDISQIVDAVPEFVEL
                     SDVSLRELLLALEKDDRLVLRDVQFPMSSDSILPSSFIVLDTLASILMDYPNLIVQLE
                     GHICCTSETGMAGHKDAYNATNGKYSLSSDRALNVFMYLVYKGVPSRQLKYVGLSGSQ
                     KLVWPEATAKDQQKNRRVEVRILEK"
     gene            15298..17796
                     /locus_tag="PLL28_05740"
     CDS             15298..17796
                     /locus_tag="PLL28_05740"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HNF68858.1"
                     /translation="MFKATLFSAISATIFSFGNELDAQDSIPKPSMPSTKVTIAPKID
                     GNLQDEVWQSVQPVTLDVTFLPEFGKTPSRKTEVRVVYDNNAIYVGAIMYDPDPATIN
                     RQLCERDGYSNADNFVFGLDTYNDDLNGYRFTVSAAGVQSDQRIGLNSNGDMSWDAVW
                     ESDVSINELGWVCEIKIPYSAVRFPSKPLQDWGMQFNRNVNSTGELILWSPVDPKVYG
                     IINQWGTYTGMENIIPPLRLSFSPYLTAGNQWTPDPENEGGTVSEGILNGGLDVKYGI
                     NESFTIDATLIPNFGEVQSDNVVLNLSPFEQQFNERRPFFTEGTEIFNQTFNFLSDRM
                     FYSRRIGGTPIFYYDVYNETGAHEIIKENPFETSLINATKFSGRTDKNVGVGVFNAIS
                     AQEFAIIEDTVTGETRTVLTNPMTNYNMLVFEKALKNNSKVSFTNTNTLREGIYRDAN
                     VSQALYDITNKKHTWNFWGFGNYSQVFEPYYSGEREVVEETKDGYNYIVGISQFTGKW
                     NFNFSHALISPEYDHTDMGIQYGYNFINNYIGFNYNNQEPKKGAFYNYSGWGGFTYNL
                     QAEPLEYQEVNYNLGFNGQFKNFWNIGGNLYSKPLWWYDFYEPRVEGRKYYHAPFTFI
                     NVWGGTDYRKDISFNWNISFGESPIPNDPYIGGGQWITWNISDKFSLTHGISLSKDHS
                     NFGFVDFDRGGNSIFGRRDITNIDHEIGMKYLFGPKMNIIFRAREYWGKVVYHQFYTL
                     LDDGNLGDTDFTGNYDLNFNIFNLDFVYSWEFAPGSYLNVIWKNNIFQADDIRYDDYF
                     DNLNKTFNAPQSNGLSLKVIYYLDYQYLHKNKQS"
     gene            17887..18282
                     /locus_tag="PLL28_05745"
     CDS             17887..18282
                     /locus_tag="PLL28_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68859.1"
                     /translation="MKKTLFLLSVAASVILVTSCESNTHWDKTFVNSSTHTLILYTTD
                     GGGGFTFADSIILAPGAEEVIYSFSDVVNDAAPACEQYINRLSLSAEEGFTITKDVSD
                     SDNWVDDSVEESHGFMHNCTFTISDSDII"
     gene            18323..>18871
                     /locus_tag="PLL28_05750"
     CDS             18323..>18871
                     /locus_tag="PLL28_05750"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNF68860.1"
                     /translation="METPEKPSFKEALTFWFKLGWISFGGPAGQIAIMHTYLVEQKRW
                     ISEKKFLHALNYCMLLPGPEAQQLATYTGWLLHGKRGGLAAGILFVLPSAFILLILSI
                     LYVQFGHIAEVNALFEGLKPAVVAIVALAMVKIGKRSLKYALHVAIAILSFVAIYFFN
                     FPFPAIIGLILLFGLLALRLKPS"
BASE COUNT         5675 a         3775 c         3967 g         5454 t
ORIGIN      
        1 aggtatcagg tatcaagtat caggtatcag gtatcaagta tcaggtatca ggtatgaagg
       61 attggagatt tgaaggtttg aaggtttgaa ggattaatcg actatttaat tatttagcct
      121 ggtaaccttg ctcgaggagt tcctgcaagc atcaaagtcc ggcaagcgtc ttaaacgaga
      181 cggaggcgcc ctaagcattg gatttgatca aactcacaaa acggaatata gaaaaagtga
      241 atggggatgc ctacctcaag gttcgatcac aaaagactaa ttgaagaaat gccgttacaa
      301 tttatgcttt aaaccaaagt aagaattatt atagttgtaa gatttacatg caaataaaaa
      361 taaaataaat tcacagagat tctagttatt aagcattttt gtatgaagtt atgaagccaa
      421 gaatttttac gctggacgaa acaacccaaa tagctaaaat gcttaatgca gaggcaaagc
      481 attctcacat ttttcttttc cacggcgaaa tgggttccgg aaaaacaaca ttgataaaat
      541 cgatgtgcag gcaacttggc gtaaatgagc atacctcctc ccccactttt gcgatcgtaa
      601 atacttatca gggaacgcag catatcatat atcacttcga ccttttcagg ctgcgcgatg
      661 tgcatgaatt ggaggccata ggattttcgg aatatgtaga ttcggggaat atgctgttta
      721 ttgaatggcc ggaacaagcc ttgccactgc tgaagggata tgaggtagtg aagatagtgc
      781 tggaaactat cgacactgag catcgcagga tttccatgca gatagaaaca ttgtgaactt
      841 tcaggcattt tgctacatga tgcccgttgc ttaattttat agcatgagcg acccgaccca
      901 gaaatctata cttaccgaat tggcagcggc tgccggatta cagacattgg agaagtatgc
      961 cgatgttgac caaaaaaaac agcgattgtt tatcggaatt cctcgtgaga caggctttca
     1021 ggaaaacagg gtgccgctga ctcctgatag tgtggagaca ttggttgcgc aaggtcatcg
     1081 cattgtgatt gaaacaaaag ccggtgaagg cgcgcggttc agcgataaag attatgccga
     1141 tgccggtgcg atggtgatgt acgatcgcaa gcaggttttt gaagcggata tcatattaaa
     1201 agttacgcct cctgccatgg atgaaatgga attgttccga catggtcagg tgctcatatc
     1261 gccattacat ctccctaccc tgcgtagaga aattattcac gacctgatga aaaaacaggt
     1321 tactgcactc gcattcgaat atgtaaaaga tgaaagcggc acgtacgcat ttgtcagatg
     1381 tatgagcgaa atcgccggtg caagcgctat tttactagca tctgaattat tagccgatgc
     1441 tattcatgga agaggatatt tattaggcgg actatcgggc attccaccta ctcaggtatt
     1501 aatacttggc gccggtgttg ttggcgaatt tgcaacacgc gccgccttgg gattgggtgc
     1561 tacggtgaaa atttatgaca acaacgtata taaattatcg cgactgcaaa acaatatcgg
     1621 cgaacgtttt tatacttctg taattaataa cagacgatta ttaaatgata tccgcaccgc
     1681 agatgtagtg gttggcgcca tacatgctga aagtggtcgc acacccgtta tcgtaactga
     1741 aagtatggta atggaaatga aacccggcag tgtgatcatc gatgtaagta tagatcaagg
     1801 tggatgtata gaaacgagtg aagtgactac acatcttcaa cctactttta ttaaacacga
     1861 tgtgatacat tattgtgtgc cgaatatcgc ttcacgtgtt ccacgaactg ccagttatgc
     1921 catcagcaat atcgccagca gcaccttaca gcaatttacc gagtatggcg gattagaccc
     1981 gctcatcaga aatctggaag gcatacgcca tggtgtttat atttataaag gacatctcac
     2041 aaattattat ctcgcacaaa aaatggaaat taaatatacc gatatcaatt tattattgcc
     2101 gagtgtatta taatttcttc ggttgacatc accataaaaa aaatcaggcc gataccatag
     2161 gcaccgacct gaacaaaacc aaacttaaaa atttattatt gtaacaccag tcgcgcagtt
     2221 gcgtttgcac catgtgcatg aagtgtaaca aagtagcaac cggccgataa atgcaaatca
     2281 gccaggttta attgaattgt ttgtggacct gttgaagtca ctgctgtttg ttgggttgtg
     2341 attacgcgtc cggtaatatc agaaacttgc acctgcacaa tacccgcagt ttctgcagta
     2401 taatgcaagc ttacaacatc ctgaacccct gtgatattag gtgttacagc caatgtgccg
     2461 ttaccgccaa atccttcgat accaagggtg ccatcagtga aattaaagtc atcaacaaat
     2521 acgttattta cataattatt gtaaacgaat tcaattttga aacgcacatt ttcagctttt
     2581 gcacctgaag gaatagagaa tgcagcaacg cgccattgat cggcaaacac agggaaaaaa
     2641 ttactgctgc cgccataaga agtgatcaga tcatcaccgg ttatttcgcc aacgcttatc
     2701 caggtttttg cacaattcgt gctgtaatac aatttgagtt tcatggtata atcatccgca
     2761 tcaaaaccat ttgaagtggt gctatatttg aaaaacacat tgtcggttaa cccgtcagat
     2821 aaatcaaagg aaggagaaat aacttcatct ttttcctggt caggaccgaa gcgactattc
     2881 aaccagatac aaggtgtatt ccaataacca ttttgtccgc gccagttcca ctggtaattg
     2941 tcgggatatc tgttatacaa tacccaatca tttaaagcag catccatatt attaaaattg
     3001 ccaacataat cggcattata cgatgctaca tcatctgata cataaatata ttgctcaaca
     3061 gtttttgttg ttgtaccact tgcattggta acctgcagtg taacactgtg ccaacccgga
     3121 gtattaaaag aaactacagg atccatttct gttgatgtca gtggagagcc tccctcaaaa
     3181 gtccacatac gagaatcaac atcaccacgc catgatgcat catgaaaagt aacgctttca
     3241 tccttacaaa caaatcgttg tgaggtgtaa aaatcagctt caggagaaca caatcctgca
     3301 acatatccat cgtttgttcc cgtaagcatg agattatctg aagaccacaa atgattgcgt
     3361 tgcgcatcgg tgctgtttaa cgcggcatac atacgcgttt tctgaccttc cgtaaacatg
     3421 gtagaacagt atgaatattc cataaaattc tgcacgttat cgagtggttc gatacaagta
     3481 tgtccggcaa gattacatga aagccaacct ttagtaaccg gcgtatcatc aacctgatca
     3541 tcgccacatt ctacacccgg attatttgta tttccccagg tgtgttgcag attgagataa
     3601 tgaccaattt catgcgataa agtatgtgaa gaatattcac ctgcggcacc aatacttcca
     3661 acataatcat agcgacaaat tacaccatct acaggtgcta aggcgtcgaa tatagaagtc
     3721 ggataatagg catacgctgc agcagaaccg gccaggaatt tcaccaccca gatattcaaa
     3781 taaaatccat gttgccatcc gccgagttta gcagcatcac cacctgtgta agtgcgcatg
     3841 gaaggaatgc gatcaatacc attggtacat gctccggaaa gttcgcgttg tgctagacgg
     3901 aattccacct gcacatcggc agcaataccc aaaaattccg taacggtttc agcaaggtcg
     3961 ctgttctctt tattgaagtc ctcatttatt ttttccatgg catcaaatat ctgttcgtca
     4021 ctgatatttt ctacaccata atcatggatc acatgaaata ccacaggtat gatataagtt
     4081 ccctcttcaa cctttaattg cgaaagatcg aatttctgtg catagatatt aaatgcacgt
     4141 tcgcgttgaa tgatttcggg atattgttca attaattgtc tgcgtacatc atctgtccca
     4201 caagtagtcg tctgttgcgc ttgcattggc cttgcaaaga ggactgtaca gcatacaaat
     4261 agtaaacgta taagtttaac tgtcataata aattgattta agaagaataa aactgaaaaa
     4321 aaaacaatgg tcaattgtca gatcagctca tccggaggat tgatttggct attgagatag
     4381 cctgaagatt ctataaactt tgatacattc gtttgcatga tcaatgtttg atcaggtacg
     4441 aaatatgcag ttaatgcaac cacatcaaat tgcgtatcga gttgcaactt gggcagcgac
     4501 tgatgttttt gatcagtgtt gttttgatgt tgcaattggc tcgctatttt ttcttccagg
     4561 tctttttcct tgtcatctct tacggcaatt atatggtagc cgtcagatgc catgtccatt
     4621 tgaatgatat cgtataaaaa accctcgtaa gtaaattcgt gtttatcatc gcgccaggag
     4681 aatccattta atgcgtgctc tacatgatca agggggatgt tgagcgttgt agcgatgtca
     4741 ttcacttcac cggcttctat gcgattccat acttcttgtt tcgcatgata tgttgcagcg
     4801 cttagccaca gcgtaaatcc attactattg atgagcaatg ttgccatcaa cgtaatgcat
     4861 ccgatatatg cgacaatttt tttcactgcc tgagatgggt aaaaatacac attcaagcga
     4921 taagcatcat caattccatg tcattttgaa aacgcttgct tactgattgt atacagtagc
     4981 gtacacgcag aaaaagaagc ccgttattca cgtcttcacc aatattcagg caatttatgt
     5041 gccaaatgtc attcattttg actttttgac attcctaatt caattatttc ggcagccggg
     5101 aattcgatgt attttaatac tgtattacat ttcattcatt ttgtgcgata agaacttccg
     5161 ggatgaggat attttttttg acaatattat ttgtttttgt cagcaaatag tcagtatatt
     5221 tgacactgat tatgcgatta tcgatggatc atagcgggcg gatgattgtg cacattgacc
     5281 tggacacctt ttttgtgtcg gtagagcggt tgaaggatga caggttgaag ggcaagcctg
     5341 tagctgtggg cggcacgggt gatcgcggtg tggtggcgtc gtgcagttat gaggcgcggg
     5401 ctttcggggt gcattcggcc atgcccatgc gtcaggcgcg gatattgtgt ccggagttgg
     5461 tgacagtgcg tggtgattat gaacattatt cgcattactc taatacgatc actgagattg
     5521 tgaaggagaa tgtgccatta tatgagaagt cgtcgatcga tgagttttat atcgacatga
     5581 cggggatgga tcgttttttc gggacctata cttacatgca ggaattgcgg gagcgcatta
     5641 tcagggaaac gggattgccg atatcatttg gcttatcgga gaacaaaacg gtatcgaagg
     5701 tagccacggg gcaggccaag cccaataatc acatgaagat tgatcatggc gtagagaaat
     5761 cttttttggc accgctgccg gtgaagaaaa ttccgatggt gggagaagta acgggacagt
     5821 tgttgcgcaa gatggggata gtgcagatca aaaccataca agagatgccg cagcaggtga
     5881 tggaaaatat tttgggcgaa aacggtcgcg tgatctggcg caaggcaaat ggtatcgata
     5941 ccagtccggt agaaccctac accgagcgca aatctatttc gagtgaagag acttttgaaa
     6001 gcgacacgat agatgtttcg cgcattcgca ccatattgat ggcgatgaca gaaaatctgg
     6061 cgtatcagtt acgaaaagaa ggaaagctca catcggtggt ggcagtaaaa atcaggtact
     6121 ccgatttcga tacgcatacc aaacaaatac ggataccata tacatcaaca gatcacactt
     6181 tgatgcgcgt ggtgaaggaa ttatttgata agctgtatga gcgacgtgta ttgatacgcc
     6241 tcgtgggtgt tcgtttatcg ggattggtga atggcggtta tcagatcaac ttactggaag
     6301 atgccgaaca taccattcgc ctctaccagg aaatggacca tgtccgcaaa cgcttcggcg
     6361 acacagtagt ccagcgcgca tcagcgatgg gatataaaat gagaagattt aatccgttta
     6421 aaggagagag atagagtgtg gaagtgtgga ggtgtgaaag tgtgcagcgg aatgcaggtt
     6481 tcatagtgtg actaaacatg aacacaaact agtgtgaaag tgtgcagcgg aatgcaggtt
     6541 tcatagtgtg actaaacatg aacacaaact agtgtggaag tgtgcagcgg aatgcaggtt
     6601 tttcagtgca cttcaacacg aacacatgag cacatgaaca caaactagtg tggaagtgtg
     6661 cagcggaatg caggtttttt agtgcacttc aacatgaaca catgaacaca tgaacacatg
     6721 aacacatgag cacatgaaca caatttacac ttccacactt gcacactttc acacttccac
     6781 actcaaatgt ttctcaacac ccactcatat tacagcttac gctacggggt tttgtcgccg
     6841 gagggattgt tggcggaagc gcagcgctgt ggggtgcatg cgtttgcgat gacggatatc
     6901 aataattgtt cgggatggtt tgaattgtat gcggaggcga agaaatttaa tatacatgtg
     6961 gtgccgggag ttgaatttaa aaatggtgat gatcatctgt atatcgggat tgcaaaaaat
     7021 gaacatggct ttgcagaact gaacagatat ttatcgcact atctgaatac taaagagcct
     7081 attcctgcca tagctcctga atttgaacat gccataatta tttatccatt cgttgccgat
     7141 aagatttatg ttttgggtga taacacattt gtcggcatac agaaaaaaga cctcagcaaa
     7201 ctgcgtgtcg gcaaatcggg gttgccacag gaaaaactgg tagcgatgct gcctgttacc
     7261 ttttcgtcga aaaaagattt tagtgcacat cgcatattgc gcgcgataga tcacaatacc
     7321 atcatcacca aaattccgga aaacgccact gccctacccg atcaggtgtt caggccgtac
     7381 gacagattgt atgaagattt tggcgatcat ggctatctgc tcgaaaatgc aagagagctg
     7441 ctggaaaatt gcacttttga tttcacctat cataccaata aaaatatccg gtcgttcagc
     7501 ggcaatgagg ctgatgatat caatcaattg ttgcaactca cctacgaagg agtgaaacgg
     7561 cgatacggca aactcaccaa gactatccaa gatcgcatca ataaagaact ggagatcatt
     7621 atacaaaaag gattcgttgc ctattttctt atcaattggg atattatccg ctacgcacat
     7681 agcaaatcgt attttacaat tggtcgcggc agtggtgcca acagtctgat tgcttattgc
     7741 atgggtatta ccgatgtaga tccgatagaa ctggatttgt atttcgaacg cttcatcaat
     7801 ttatttcgcg aaacaccccc tgatttcgac ctggattttt cgtggaaaga tcgtgaagat
     7861 gttacggatt atatcttcaa acgctatggc gaagaacata ccgttttgct ggcaacatat
     7921 accactttta aaagtcgctc gtttatccgg gaggtagcaa aaacattcgg actgccgaaa
     7981 tatgagatcg atgcgatgat taaaaattat ggtgaaagta tttttggcga cagcatacag
     8041 attcccgata aaattgcacg cagcatttat aatttcggtg cttatttgca agggaatcca
     8101 aactacctgg gtatccatgc cggtggtatc ttgatatcgg agttgccggt gtattattat
     8161 accgcaacac atcttccccc caaaggattt cctaccacac aattcgacat gcatgtggcg
     8221 gaagatatcg gcttatataa attcgatatt ttgagccagc gcggactggg acatatcaaa
     8281 gaaaccgtag aaattgtaaa acaaaatcgc ggtgatgaca tcgacattac acgcgtgcag
     8341 gatttcaaaa aagatccggt gctgaataat aatctggtga agggaaatac gataggatgc
     8401 ttttatatcg aatcgcctgc catgcgcagt ttactcaata aattgttgtg cgataattat
     8461 ctcaccttgg ttgcggcgag ttccattatt cgtccgggtg tggcgcaatc gggcatgatg
     8521 gatgaatata tccgcaggca tcgctttcct gaagaacgca agcgcataca tcctgttatg
     8581 ggcgatatta tgcccgatac atatggcgtg atggtgtatc aggaagatgt aatcaaagta
     8641 gcgcactatt ttgcgaaact cacattgagt gaagcagatg tactgcgtcg gggcatgagt
     8701 ggaaaaagca ggagtaaaga tgaatttcaa cgcatcaaaa caaaatattt cgagaactgt
     8761 gcgcaattag gttatgataa agcacttgcc aacgatgtat ggcatcaaat agagagtttt
     8821 gctggttatt catttgcaaa aggacactct gcatcttatg ccgtagaaag ctatcagagt
     8881 ttgcatttga aaagttatta tccgctagaa tttatggttg ccgtaatcaa taatttcggc
     8941 ggatattaca gcaccgaatt atattttcac gaagcgcgaa aaagtggcgc caccattgaa
     9001 gcaccgtgta tcaataacag ccggtataac acttgcattt acggcacggt gatctatgta
     9061 ggatttgtgc atttatccgg actagaatcg gcactcgctg agcaaatgct tgccgagcgt
     9121 gcgcacaatg ggccttttac ttccttcgac aattttctcg atcgcgtggc catcaatctc
     9181 gagcaactgg aattattaat caccatcaat gcttttcgct tcacgggtat caccaaacgc
     9241 gagttgctga tagaagcata tacgcgcatg aacaaaacaa aaaaaacaat tcctgccgct
     9301 accttgttcg aaaaaagtgc gccaccgaaa tacacctttc ccccactcca cttcgatgcc
     9361 cgtgaagatg cgtgggatga aatcaaatta ttgggatttc cactgtcatc gcccttcctt
     9421 ttaattagcg atccgattat tgaaaatcgc aatttaaaac tggtaaaaga tctgccgaaa
     9481 ttcgttggta aaacggtaga gatcatcggt tatctcatca cgacaaaacg cacttcaaca
     9541 aaacacggtg agccgatgta tcttggcacc ttcatcgata tggaaggcaa ctggttagat
     9601 accgcgcatt ttccggcggt agcaaaacaa tatcgcttta aaggaaaagc ttgctatctc
     9661 ctaaaaggaa aagtgcaaga aagttttcaa gcgcatacac tgattgtttc tgagatgtat
     9721 catttgaaga tgtgggtgga gggggagaat tagccgatta gccaattagc caattagcca
     9781 attagccgat tagctgatta gcagataaat gcggaggaat gtaagtgtgc aagtgtggaa
     9841 gtgtgcaagt gtggaagtgt gggagtgtgg aagtatgcta gtgtgcaagt atgcaagtaa
     9901 atggtaacgt ttaattagcc aattagccga tttgggttcg ctctgcgcct ctgcgcctct
     9961 gcgtgaaaaa ccctccacac cacaatcacc tgtcacgact cacccttgtt taatcgcgga
    10021 gtttgcagag ttagcggagt cacatcagtc accactcacc attcaccatt caccgaaagt
    10081 cccctagtcg gatatcacta cgcttcaatt tcaggagtaa ttaatagtga atcttccctt
    10141 cttacataat cgtatactgc gtgccggaac ccgtaccgtg tttttcaata agttttttct
    10201 caagcaggga ggaaagcaat ctaattacaa tcattctgga gaaacccaac ccttttgata
    10261 tttgtccgga tttgcaaccg ggatgatttt gaatgaaact tacgactaat ttttcgcgtg
    10321 ggagcaattg atctgtttca gtttcataaa tgagtagttt ccgctctaac aattcctgaa
    10381 tattttttaa tgcgtcaaaa aagaaattta cccaagtgct tatatctata ttcggacgca
    10441 aggtctgaca gcttcgcagc acttgatagt aagcatgttt tctgctttca atttcgtgtt
    10501 caaaactgat atattgaatc catttatatc ctgactttaa taagagtaaa gatgaaagta
    10561 atcgactcaa tcttccgttc ccatcctgaa atggatgtat actcaaaaat tcataagtaa
    10621 aaacagcaca tttaaccaga gggtgaatcg cgtgttcatt gttgtaccaa ttcactaaga
    10681 gcctcattgc agtttcagtt tcaacacccg gttcagttgt ttgaaaaaga atttgcctgg
    10741 ttccatcagt gtacctggcc tcaacagcat tgctatgttg cttgtagttg cctttatgcc
    10801 attcatcctt ggcactgtac ttcatcaaaa tattatgtaa atttttgatg tcgctttctg
    10861 taatttttat ttcttcaaat tgttcggaaa tgatatctaa agcttcaaaa taaccaacta
    10921 cctcctgggt gtctctgtct tcaagcttgg agatatcgat atttctcaat aaaacatcta
    10981 cctcttcatc gctcaattta gacccttcaa ttctggttga agcaccaaca cttctaactg
    11041 tagcaattga tttaagctct tttaaaccct gttgctctct cctttcaatg gccgtccagg
    11101 cagcatcaaa tctgtcgatc tgactaatca atttaatcag ctcccagtct aaattgagat
    11161 tgaatatgta tacgttactc ttcattattc aaattaaaat gatatgaaat gatacaatat
    11221 gatacaaagt tatacaatat gatacaaata attgcaaaaa gttacgattt cattcaaatt
    11281 caataaaaga tcaggataat cttagatata tgtcattgat taacaattaa ttagttgcga
    11341 taaacgggat ttatatagaa aacaaaatag tcctaatgaa tatagatgga acaggtgaaa
    11401 tgattatctt agaataatta acttcctatt taggccccca atgaaaccga aactcacact
    11461 attgcttttg attccacttt tggctatgtc gtttttaatt aaaaatgatc cacaaactaa
    11521 cgagatcaat ttgaacacat tagaccaaca actgattggt gaatgggaat acaagatatc
    11581 cttttcagat tcaagttatt ttactgtgcc atttaataga atcctaccgg tacaaaagtt
    11641 gagatttgag cattgccatg attcttctga attagaacgg tttcccaaaa ttgtgattgc
    11701 tatgaggaaa cacaattcct tggaaaacat ttcttgcaaa gcatacaacg aaaacaatga
    11761 ggtgattgac acctactacc ccgttgtatt gtttggtaac gattctctgg taaatcagat
    11821 tgatgtcagc caatacggac atatcctaaa gtctgaatat acaatttata cattaacttc
    11881 agatagccta attattgggg atgggaaatt gtttgaatct ccagatagga aaatggtact
    11941 tgtcagacat atttatttaa gaaaaaatta aatattatgt cgtggtttca ttttaaactg
    12001 taaaagaata ttatatttga aatatcaata atataaatgc aggatccatg gctttaatag
    12061 aagaagaaaa tcacaccctt aaatcatgag ccgactaatc ctctcgctaa ttgccactgt
    12121 cacttttatc ttattgactt caatgagtgc ttgcatgaaa tacgacatag aacgtagggc
    12181 aaatccttca aatattgaag tttatgattc aatctattta aagaacggga attattgctt
    12241 ttggtatgcc tataacctag gaggaattta tggaggaatc ggcgattaca tttctatagc
    12301 accccaaaga tctcaaatat ccgactctac tcaagtcatc gtatcagaat atatcaaagg
    12361 tattagggcc atcagtgaaa acgaaatagt tgtaatcttg acagaggacg aatatgatga
    12421 aatacatacc acatttaaag gaattaaaat aagtgcaaaa attgattaaa atataaacag
    12481 tttgcgtgta aacaaatgca tcaacttatg aaattgataa acaatcccat aacaaacctt
    12541 ttgcaatttc tcgaataaac taaaatcatt agcggattgt ttcaacttca cactcaaaac
    12601 aactccccct gcacaggctt ttgtggtttt ttatcggcgt attccatgag catatgggcg
    12661 ggactgaagg gtgttgtgta attagccagt agacttttta aatcggcatt cggatctaac
    12721 cagggcgatt cggttcctat gggaataata acaggcatgc ggttatggat actcgcaatc
    12781 aaactattcg gcgtggtggt gattactgcc atcgtgcgaa tgatctcgcc ggttttttta
    12841 ttgatgtagt tattatataa ccctgcataa aagaaatttt cagcatcctg taaatggatg
    12901 aacatcttgc gtttctctgc cttattatgt tcgaaaaacc cattggtgca tacgaggcaa
    12961 cgctgaccat gcttccatgc atcgcgccag gttgctaact cggtaatggt ttcaatacgc
    13021 gcattgaaag ttgccttttg ttcatttact ttttcaggtg ctgtccaatg gggaataaat
    13081 ccaaattgat aggcctgaat aatttgtggt cgctctatcg taaccacagg catcggcgtg
    13141 ccgggaaatg caatggcatt attaccactt tcatcgtgat atttttcctc tatgccaaga
    13201 tcctcttctg cccaaggtct gccgaagctt tgttcataaa cctctttaac cattttcgat
    13261 tttgtggtgt agctcgaaca catgtcgcaa atgtaatcaa caccatcgat cagttggaga
    13321 aatgcccgga atatcggaac tttgaagatg gctatttctt tgcgttttgt tgtaacacta
    13381 gcggcgttaa cctgcggttt gaccattggt ggagcaggat gcaccatgga ttctgatacc
    13441 actacccaaa acattccgca accggaacat gttgtcattg tgatgtttga aaacaaagac
    13501 tttgctcagg tgtacgatac cagcattgca ccctttcttc aaactttgat ttacgacccc
    13561 cacactgccg tatttacaaa ttctcttgcc atgggtcacc ccagccagcc caattacatc
    13621 tggtggtttt caggcgacga tcaaggtgtt accacaaatg atcgtcctgc aacaaaattc
    13681 aataccccaa acctggcagc agaattgatt caaaatggtt tctccttcgt cacctacagc
    13741 gaaaatttgc catttgttgg ttttgatggc gactcttctg aaaacggact gtatgttcga
    13801 cgacacaacc ctgtttccaa ttggataggc cttgatgtca atcaagtttc acctgatgta
    13861 agtcagccct tttcagcctt ccctgccaat ttcgatcacc tgcctaccgt tagttttgtt
    13921 gtgccgaaca tgatcaatgg aatgcacaac ggcactatca ccgaaggcga tacctggctg
    13981 caaaatagct tatcggaata cattgaatat tgtcgcaata atccttctct tttggttgtc
    14041 acttttgatg aaacaactta taatgttacc gacaatcgca tacttacctt gtgctttggt
    14101 tcgttagtta atggcggaga atatgatgcg gctgtttcgc acgtgaattt tctgcgcaca
    14161 tttgaagaca tgttttcatt accacacgcc ggattatccg gatacctgga accaatcagc
    14221 aattgctggg tgcaggaata attatgcgct gtaaaaaatt atttattggt ttagtaacgc
    14281 tgactttaag ctggtttccg tcgacagttt ttagtcagga taccatacgc gcccatattt
    14341 atttcaaatc gggatcatac gaaacgaaac cgcacaacac ttatctgcag cgatttgcag
    14401 acgcttgcgc aggatttgat tctgcacata tttttatcga cggcttttgc gatgacgaag
    14461 gaagtgatgc cttcaacata caactctcag aacaacgtgc tatggctgtg gaggcttttt
    14521 tgaaacaata tattaccatc aaagcaaatt ttaccatcac cggtcatggc gaatcaaatc
    14581 cggttgcaag taatgaattt gatgacacca aatttcaaaa tcgcagggtg gatatgcttg
    14641 tcattggtta tcagtacggt tatacttctt ccataagtga tatttcacaa attgtcgatg
    14701 ctgtgcccga gtttgttgaa ttatctgatg tatccctgcg tgagttattg cttgccttag
    14761 aaaaagatga tcggttggtg ctgcgcgatg tgcaattccc tatgtcgagt gattcgattt
    14821 taccatcgtc atttattgtt ctggatacac ttgcaagcat tttgatggat tatcccaatt
    14881 tgatagtaca attggaagga catatctgtt gcacctccga aaccggtatg gccgggcata
    14941 aggacgccta taatgctacg aatggcaaat acagtttatc ctcagacaga gcactgaacg
    15001 tattcatgta tctggtttac aagggagtac cttcccgaca attgaaatat gtaggacttt
    15061 cgggttctca aaaattggta tggcctgagg ctactgcaaa agaccagcag aaaaatcgac
    15121 gcgtagaagt gcgcatactc gaaaaataat gcccattttt taccacaacc cctgtgtata
    15181 aagcccttat gcctgttgat ttaacagcat ttaacccaac attaaccgat agaaatatca
    15241 cattaacctt ttttatcaaa tgttgcaagt ttctttgcaa ttattaaaac ccaatacatg
    15301 tttaaagcca cacttttcag tgccatctcc gcgacaattt tttcatttgg caacgaactg
    15361 gatgcacaag acagcatacc taaaccgagt atgccatcca caaaagttac catcgcacca
    15421 aagatcgatg gcaacctgca ggatgaagtg tggcagtctg ttcagccggt aactctggat
    15481 gttaccttcc taccggaatt cggaaaaaca ccgagcagaa aaactgaggt gagggtagtt
    15541 tatgacaata atgccattta tgttggtgca atcatgtatg atcctgatcc tgccactatc
    15601 aataggcaac tttgtgagcg tgatggatat tcaaatgccg ataattttgt ttttggttta
    15661 gatacttaca atgatgatct caacggctat cgcttcactg tcagtgctgc cggtgttcaa
    15721 agcgatcagc gcattggact gaacagcaat ggcgatatga gttgggatgc agtatgggaa
    15781 agtgatgtta gcatcaatga gctgggatgg gtatgtgaaa taaaaatccc ctactccgca
    15841 gtaaggtttc cgtcaaaacc gctgcaggac tggggaatgc aattcaaccg aaatgtcaac
    15901 tcaacaggag aattaatttt atggagtcct gttgacccta aagtatatgg tattattaat
    15961 caatggggta cctataccgg aatggaaaat attattccac cattgcgatt gtcgttttca
    16021 ccatatttaa ctgcaggaaa tcaatggaca cccgacccgg aaaatgaagg tggaacagta
    16081 agtgaaggca ttttaaacgg cggcttagat gtaaaatacg gtatcaacga aagttttacc
    16141 atcgatgcta cacttattcc gaatttcggc gaagtgcaga gcgataatgt agtattaaac
    16201 ttatcgccgt ttgaacaaca attcaatgag cgcagaccat tttttactga aggaacagaa
    16261 atttttaatc agacctttaa tttcctcagt gatcgcatgt tttattcgcg acgcattggc
    16321 ggcacaccca tattttatta cgatgtatat aatgaaacag gagcacatga aatcattaaa
    16381 gaaaatcctt tcgaaacaag tcttatcaat gctacaaaat tctccgggcg caccgataaa
    16441 aatgtaggtg tcggcgtatt taatgctatt tctgctcagg agtttgcaat tatagaagat
    16501 actgttaccg gagaaacaag aacagtgctg accaatccaa tgaccaacta taatatgctg
    16561 gtgtttgaaa aggcattaaa aaacaatagc aaggtcagct ttaccaatac caacacactt
    16621 cgcgaaggca tttatcgcga tgccaatgtt tcgcaagcgc tgtatgacat taccaacaaa
    16681 aaacatacct ggaatttttg gggatttgga aattacagtc aggtatttga gccatattac
    16741 agcggtgaaa gagaagtagt agaagaaacc aaggatggtt ataattacat tgtcggcatt
    16801 tctcaattca ccggaaaatg gaatttcaat ttttcacatg ccttaattag tcctgaatat
    16861 gatcatacgg atatggggat tcaatatggt tataatttca ttaataatta tatcggattt
    16921 aattacaata atcaggaacc gaaaaaaggt gctttttata attatagcgg ctggggcgga
    16981 tttacatata acttacaagc agaacccctg gaatatcagg aagtgaatta taatcttgga
    17041 ttcaacggtc agtttaaaaa tttctggaat attggcggaa acttatattc taaaccattg
    17101 tggtggtatg atttttacga accacgtgta gaaggaagaa aatattatca cgcacccttc
    17161 acctttatca atgtttgggg tggtaccgat tatcgcaagg atatttcttt taattggaat
    17221 atcagtttcg gagagtctcc tatacctaac gatccataca ttggcggtgg tcaatggatc
    17281 acatggaaca taagcgataa attctcgctg acgcatggaa taagcttgag taaagatcat
    17341 agcaatttcg gctttgttga ttttgatcgc ggaggtaaca gtattttcgg aagaagagat
    17401 atcacgaaca ttgatcatga aattggtatg aaatatttat tcggtccgaa gatgaatatc
    17461 attttcagag caagagaata ttggggaaaa gtggtgtatc atcaatttta tactttgctc
    17521 gatgatggta atcttggcga taccgatttc acaggaaact atgacttgaa tttcaacatc
    17581 ttcaacctcg acttcgtata ttcctgggag tttgctccgg gaagttatct gaatgtaatt
    17641 tggaagaata atattttcca ggccgacgat atcagatatg acgattattt tgacaatctg
    17701 aataaaacat ttaacgcacc gcaatcaaat ggtttatcac tgaaggtgat ctactatctc
    17761 gattatcaat acctgcataa aaacaaacaa tcttgatttc ggtcatggca gccgcacttt
    17821 agtgacacaa gtgttcacat atgtggtttt gtggtttcag gggcttggac tatctttccg
    17881 attgtcatga aaaagacctt attcctcctg tcggtagccg cctcagtgat cctggttacc
    17941 tcctgtgaat cgaatacaca ttgggacaaa acctttgtaa acagcagcac ccatacgctg
    18001 atactgtata ccacggatgg aggcggaggc tttacctttg ctgattctat cattctggct
    18061 cccggagctg aagaggtgat ttacagcttt agcgatgtag ttaatgatgc cgctcccgct
    18121 tgcgaacagt atattaaccg cttaagtcta agcgccgaag aggggtttac catcactaaa
    18181 gacgtttctg attccgacaa ctgggttgac gatagtgttg aggaaagcca cggattcatg
    18241 cataactgca catttacgat ttcagactcg gatatcatct gaaacctgcg gaaacgaagc
    18301 ttccaatggc attagaagaa acatggaaac accggaaaaa ccaagtttta aggaagcgct
    18361 taccttttgg tttaaattag gttggataag cttcggtggt cctgccggac agattgccat
    18421 catgcatacc tatctggtgg aacaaaagcg ttggattagc gagaaaaagt tcctccatgc
    18481 cctcaattac tgtatgcttc ttcccggacc ggaagcacaa caattggcaa cgtatacggg
    18541 ctggttgctg catggcaaaa gaggaggttt agcagccggc atactttttg tattgccatc
    18601 agcatttatc ttattgattc tcagcatttt atatgttcaa tttggccata ttgctgaggt
    18661 aaacgccctt tttgaaggac taaaaccggc agtggtggca atcgttgcac ttgcaatggt
    18721 gaagatcggg aaacggtcgc tcaaatatgc cttacacgtt gcaatagcaa ttttgagttt
    18781 tgtagctatt tactttttca actttccatt tccggccata atcggcctta ttttgctgtt
    18841 tggcttactg gctttgcggt tgaaaccatc g
//
LOCUS       DAOQHY010000049        18693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1355_length_18693_cov_6.383571, whole genome shotgun sequence.
ACCESSION   DAOQHY010000049 DAOQHY010000000
VERSION     DAOQHY010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 18693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18693
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1355_length_18693_cov_6.383571"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2030)
                     /locus_tag="PLL28_05755"
     CDS             complement(<1..2030)
                     /locus_tag="PLL28_05755"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNF68861.1"
                     /translation="MKIRILVLLLLAVATNVLSAQESLLWKVTSPNGKFTSYILCSTE
                     LPGVENYDIITPATPIISKVNTVAFFNVPDAAEIQNIPTFMKAPGDNTLKGYYKREDR
                     IRFELMVADKLKENIETYYAYKPLYTLQLMREKDHAAGLDYQQTILLDIALQQKKPTL
                     SLMTIRQIAGVMDLMDFNTQATVLSNYINTASLYIDADNEKFQAYAGQSMKDYLRVVN
                     SSEQDAYITTMIGSMNDMLMKKMDPLLQQQSALFILDADLIGGDLGIIKKLQAKGYTI
                     VAEPFDYKKYADGMKPDPNLTNNTINNATASNIPADDFPSFAIQPVTGGYVDPETIKV
                     INIKDNPGKKPGYLAYRDPFGDFFDMQSADTLFMESWYELRGTDANFHVKVPVKGDWE
                     ESSTPWLEGGEIKKFVYQTNHAKSDVFYSIGYTVYPPSFKADDRDAFFNQFIQSTQDQ
                     ISGQIISQRIVSNPNFTGREFTAVVGDSFFVRSQFLLQDNVLYQLLVGGPGDNSYSVY
                     AEAFLNSFQTASNVLVNWFFFEQPSFSCYLPMEPSKSTKTYTLPSGPLQVQTFSAPDL
                     KEAIEYQVVVSTYPAGHKFSNKKTFFDELIANAEKQYIGKALKVESVENNGIEGRYVE
                     MQLMNRKIYRMYIYFDGTSTYQFVAGGDSNVMVSNNVNRFIQSMKMSAAKKK"
     gene            2112..2630
                     /locus_tag="PLL28_05760"
     CDS             2112..2630
                     /locus_tag="PLL28_05760"
                     /inference="COORDINATES: protein motif:HMM:NF016154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HNF68862.1"
                     /translation="MDIFEISGEGAIEILIRVFIVYLVVVLGMRLVGKREIAQLSVVD
                     FVMMLLISNAVQNAMLGDNTSLSGGIIAALALFLINFVLRTLEYRNKRLKGLLEGEPV
                     TLVYQGRLLEGNLKKARITEAELMSAIREHGVNEIGQVDLGVLEINGSISILSNDFSK
                     RSSTEKNEDVKK"
     gene            2617..3555
                     /locus_tag="PLL28_05765"
     CDS             2617..3555
                     /locus_tag="PLL28_05765"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68863.1"
                     /translation="MSRSNNRNMQHLSFAKNTITGIIFFACVQFTNAQCVVGDCINGN
                     GTYKLENGDMYTGNWINGVREGYGRYDWASGAYYVGNFKNNMLDGEGAFYGVDGTKMI
                     GIFIKNNYAGTAADTANRKIYSPDDEIQKAYNDIKKRDSLAKATTLAKAERVEFCSLL
                     RRLTTDFPNQFSSFLGERQEIVLSNSKNWYATIMATGSLEAGVNSFSGKPERSYYNIL
                     FESVDSVSALAQYAQYVEQVKTCNNGCCTMVYDESTFHAKSQSGYSTIWATLAVKEGL
                     EPLLYNNLIIEIECLTKMAEPGWIVVLRMYDAQTLK"
     gene            3676..4596
                     /locus_tag="PLL28_05770"
     CDS             3676..4596
                     /locus_tag="PLL28_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68864.1"
                     /translation="MKQFFAAITLVFFVQLADAQGFVFDSVGVQHMTIGKVMLKSDTW
                     VIRVERGGGYDDFWPVNLPEKYAIAGQEVVFEGALGRIPMQARLVGTPVKLSMIRVLY
                     RTTPRDDDKSGESIAKEKPINPDSVGYIEKQTGRILMVNDMYLIETTTVDAKGNEEVK
                     RYVPDFLPDDFKMAGQQITFSAIILKHDPNVRMMGTPVKIKELMMEENTPFDATQLQE
                     SVAPYYPFDSAGRLVETKAMVKLIADVYILEVTIDNQNNITRYIPAILPESFKKEGTI
                     VMISGTIGKIPPNVRMMGTPLTLESIRLVE"
     assembly_gap    4709..4808
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4971..5741)
                     /locus_tag="PLL28_05775"
     CDS             complement(4971..5741)
                     /locus_tag="PLL28_05775"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNF68865.1"
                     /translation="MKQIIFAICCVALLSNCSEKKSETTEDIVPQKTDTVISTTTPEQ
                     IQSQTTTTDTMDDNKVYYVNISTTYGDMLVKLYNETPAHRDNFIKLVKEGYYNDLLFH
                     RVIKDFMIQGGDPDSRNAGPNVQLGAGGPNYKIPAEIKPGLYHKKGALAAARQADQVN
                     PSRSSSGSQFYIVQGKRSTEAELNNITRGYGVNFSPEQKKVYETIGGTPFLDGSYTVF
                     GEVVKGLEVIDKIAAVKTMYGDRPAEDVKMKITIAEGK"
     gene            complement(5738..6100)
                     /locus_tag="PLL28_05780"
     CDS             complement(5738..6100)
                     /locus_tag="PLL28_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68866.1"
                     /translation="MSLTHEEIPIDFDPRWIAVLQRLRDQFGKKPELETILFLIGMNE
                     LGKVEDKFSKEQKQDLMHIAVCRLLSADGYYRLAGWDDDQWPMWEATKELPAWSAAEQ
                     EIIIKQHIIRYFEENKLL"
     gene            complement(6206..6562)
                     /locus_tag="PLL28_05785"
     CDS             complement(6206..6562)
                     /locus_tag="PLL28_05785"
                     /inference="COORDINATES: protein motif:HMM:NF017470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HNF68867.1"
                     /translation="MFTAPFSFEGRINRAEYGKSFGLYIVVAIIMNAIIANMLSQGAL
                     FLLLLYIPLVWFFLAQSVKRAHDLGNSGWYVLIPFYTIYLLFPLGQHGSNKYGPNPDG
                     IGEDTYYSDNAPIDKV"
     gene            complement(6811..7533)
                     /locus_tag="PLL28_05790"
     CDS             complement(6811..7533)
                     /locus_tag="PLL28_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68868.1"
                     /translation="MRTFIVFILLVFVCNVLRAQSNAKFNMGVGYGLGTHITNDGLNF
                     VIGRYNDTRPYLSKPMDEVHFFRGWNVAVETYFPKFLMEFEFIGHTSNVSAESSSTSD
                     ARDFKYRMNTFNMGYAYQLTGQKDKVQGLYGGFDISTISVKNFTRTYTIGNDAGDFYE
                     INWDLIIGVSPYLQYAGGRFTFKGYYQWMIFKQNFWDVNVALNPATWSGDAYESNEGK
                     ANTIGMVLRVNLWENDRPKSYY"
     gene            complement(7596..8429)
                     /locus_tag="PLL28_05795"
     CDS             complement(7596..8429)
                     /locus_tag="PLL28_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68869.1"
                     /translation="MAFLFSGFRSAAQTTATTLRIDTLPKGTFVIIADVNEKDPHFKK
                     KKNFIGDTIYLLSEAIIDTKGWYAGTFNLDGAYEDYFYFSFLPVAGQQYEPQKQVAAP
                     ISEEFRQGPLSFDASAVNKFINELYQAYRDSFKLVTGTYVSFSEGYTTTLQFPGAVEN
                     NLSAIDPSLGYYAYLGDYANEQDGYAAYTALLEAIRQVKVYPDIYSDAYYFPSMGAAK
                     AFASGETLYSNVGFLMQDIGAVINVQGDESVIIEVTLMTNFKDGYFLTFDFYNKILAG
                     F"
     gene            8559..9656
                     /locus_tag="PLL28_05800"
     CDS             8559..9656
                     /locus_tag="PLL28_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68870.1"
                     /translation="MKNLQHIPNITPQLNAVRARLQHTQDLIQARAAAHVLHPNEYPL
                     PADGNSLEHHFVEVYQLLSDKGKKNFEENAKLIMGYMTADRRAELGDLANLNHKSPLS
                     TVEQVKQLGTNDTMRIKQEHVTANETLLRKHLPKSFPGIAFEGPLREVDVRGQNPRTL
                     SFFVDSIHCEKTTDLRKDEISLFGFTFDAFGQGTKVEQFEVGKFKKGETIALGSKAKL
                     RDFDLRAPGFFPQSFFTNLFLFERDWVNFEKTGKRLMMLGAALSATFLTVGFYLLTLS
                     PIPVLGTALGIASIACLLMTAISYGFALLIGAMCEDVSGGAGTAFTFNIPPVIPVGTA
                     FTETLDFTIEGGLFGVHKAKYSAQVRWERTA"
     gene            complement(9666..11003)
                     /locus_tag="PLL28_05805"
     CDS             complement(9666..11003)
                     /locus_tag="PLL28_05805"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF68871.1"
                     /translation="MKALPFLLLVTGTILTNVIFAQMTTVSSDKEWEKQEVFLKNTME
                     AEYIIRLGDVDNLGFGWPEGFDPFCGRMTDSHAYPWEPQANDLPGFDRILMSSKWHGM
                     QDFPCGAEGYSFADPSMQYKPVVWNLPMNEMKDATVQNAWLQLFIDDFQAPSFCSKYI
                     ILVNGKHFLEAEKMLNAIDQTGPVGKLISIPFPEDWYPLFANGGNCMISIDEVNGVGD
                     GFALDFVRLLVNRKRENSCKGNVRGVVMIKDTEIPIANATVFTSNQSFAKTNANGEFI
                     IADIPTGFDVVSASAEGYIDGSATADIGQGDDNDPIIIYLEKGKSAKFDNNNITVGQS
                     ITLNNILFDQGKADLRPESKTELEKIVAFMQTNPSAEIELSGHTSSEGDYAMNKSLSY
                     KRVKACKDYIVSKGISESRIMAVGFGPDKPVAPNDTESNRAKNRRVEMRVTKL"
     gene            11188..12573
                     /locus_tag="PLL28_05810"
     CDS             11188..12573
                     /locus_tag="PLL28_05810"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNF68872.1"
                     /translation="MKTGIILFLIMYSGLVNAQMTITTLPDDNTEHAGTWLQWPHHLT
                     YGTIYRDRMEPCFTAMTAALVPTEHVNIIAYNNTEKNRIISVLNANAVPLANITFYII
                     QTNDCWARDNGPMYVYTNAGQLKILDWGFDGWGNDAPYTKDNAVPKKISDQTAVPRIN
                     LSAMELEGGAVCVDGAGTFLATRSSIQGDGRNPALTETDINNYLIQYLGVSNIVWLDG
                     LYGGGLDITDTHIDGFATFLDSTTLVTLSNSDLSYWGITSADRTILNNMTNANGNVYT
                     KVILPLTNKNVKTTWGANVGFKGSYNNYYVCNSVVLVPTYSDVNDATALSIIQSLYPS
                     KTVVGIDCRNAYFYGGMVHCLTQQQPVNPTPRMEYCASTENNAHLTIYPNPASDNIIV
                     QINSNDGQTVRISILDMNGKTMLEMPAYALLEGNNQIDLQVSWLPAGLYMVRISGDVE
                     TQRNGVFQVVH"
     gene            complement(12703..13548)
                     /locus_tag="PLL28_05815"
     CDS             complement(12703..13548)
                     /locus_tag="PLL28_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68873.1"
                     /translation="MKEDLEAIKHLQNNTPIEAFEGLTPSEMHCLLYNVYDKESSLQM
                     VDTIDDAILDQIPIFRIAEVFLHIIQRENGIKLTLGGSLPVKVLTELYNYGYILEDFI
                     EKGYVKLRREQDCAAILSARLTVELTGLVKRTNGKLTLTKAGIKLLNKYDRNKFFHLF
                     MQAFTNKFAWSCNDGYGEDPVAQFGWAFSVILLDKYGDKPQEAIFYSEKFLNAFPNSV
                     LFFQPGLSTPEHFYASCYELRTFHRFFEWFGFVNVEGQHNLLTREFKAYTKSEIINKI
                     FRIVQ"
     gene            complement(13803..14003)
                     /locus_tag="PLL28_05820"
     CDS             complement(13803..14003)
                     /locus_tag="PLL28_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNF68874.1"
                     /translation="MKFNLAKREKELIKHCCSELTYKEIADKMSVSQRTVDGYRESVF
                     SKLDLKSRTGIVLFAVHQGWLG"
     gene            complement(14087..14482)
                     /locus_tag="PLL28_05825"
     CDS             complement(14087..14482)
                     /locus_tag="PLL28_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68875.1"
                     /translation="MKTKLKEVLDLIGQNDYVYYTRKAFVYFSEWLAYLFTLIFTFLG
                     FYLTGYLSSEFNIAEKLSKESENPDANNYATLIKYSVLIFCLLIALVYFIYARRMRAN
                     RHFRMRVKSAERKLKVLVDDFDVVMNEMN"
     gene            complement(14632..15579)
                     /locus_tag="PLL28_05830"
     CDS             complement(14632..15579)
                     /locus_tag="PLL28_05830"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HNF68876.1"
                     /translation="MMKNKCLYCYESLESENDFHEKCSMQFFGTSVPPEIPYSLDQMD
                     ELAKQVVERSVAVPGVQAKLSMSLIKKTKEEKAVRLTVVDALGGQYIFKPPSDRYPEM
                     PENEHATMRIAESFGIQVMQSSLIRLQSGELSYITKRADRTVAGTKIHMIDMFQITEA
                     FDKYRSSMEKVGKALNQYSSNPLLDCTYYLDLAIFSYLTGNNDMHLKNFSMIEGPSGW
                     VLSPAYDLLNVAIILPEDKEELALTLAGKKRKLMREHFEQLGASLGLTPKQINGSFAR
                     MLENNSKAIEWIDKSFLSPDMKTMYIELLVKRYGQLGLN"
     gene            complement(15566..15901)
                     /locus_tag="PLL28_05835"
     CDS             complement(15566..15901)
                     /locus_tag="PLL28_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HNF68877.1"
                     /translation="MRRGKVFYKDHLAGIITETNEGEFVFQYDEAYVIDHPDDFITFT
                     MPVRNQPYTEKRLFPFFEGLIPEGWLLNIAAENWRIQTNDRMGLLLACCKNCIGAVSV
                     EQIMVDDEK"
     gene            complement(15898..16107)
                     /locus_tag="PLL28_05840"
     CDS             complement(15898..16107)
                     /locus_tag="PLL28_05840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Y4mF family
                     antitoxin"
                     /protein_id="HNF68878.1"
                     /translation="MKQLSEFVKERRKEVNLTQEEFAERSGVALTVIRKIEQGKTNLN
                     MDKVNLVLRMFGHELAPVKIKDIDV"
     gene            16531..17061
                     /locus_tag="PLL28_05845"
     CDS             16531..17061
                     /locus_tag="PLL28_05845"
                     /inference="COORDINATES: protein motif:HMM:NF039671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6155 family protein"
                     /protein_id="HNF68879.1"
                     /translation="MPFATVKKKFQKLDKKQLVDLIGNLYKLNKSNQEYLDHFAEPDE
                     AALFHKYKEQIIAAFYPPGNRPIDIPNARKALMAFQKLHPSPEYAASLLLVYVETGIS
                     FCNDFGYVDDPILNSLSVKFEDALKFIEKENMIDTFKSKAFQITHDASDIYTPISDYF
                     ADLCQYYLGSNPHDPE"
     gene            17187..18293
                     /locus_tag="PLL28_05850"
     CDS             17187..18293
                     /locus_tag="PLL28_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68880.1"
                     /translation="MLKMLSLFWCLMTITLCFAQDCKLNKSLKVPTNRQVYMLGDFHL
                     DAKQYIHAEDSTIKNTIAAEDALRNYLVDKCDVKHFFMESSVSFEYFFNKYFETGDTG
                     WIFIFNQQKYEIAKLESLRSIYVKHPDISVNCIDVVQESYVPQMLMTLYALSFYEHFQ
                     EYYTPPLNNTPLFPPWNDINVGIRLLDSLPQCNDTITPFLGAIIQSLIAPDVNTNYNL
                     YYLFRLTLQSAQAQEALQKFYGDDYSYVMRIIRGYVFGYGKSLDDILFKYRQQREDVI
                     EETVKQLLVDNNSGAYLIQMGNAHLDADSTANMLRYRLMQNNITAPFCFYNVQSMAKD
                     FFEKTFHTTPLDFSFSSSYCFHRGPGDNVYVLVK"
     gene            complement(18435..>18693)
                     /locus_tag="PLL28_05855"
     CDS             complement(18435..>18693)
                     /locus_tag="PLL28_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68881.1"
                     /translation="DLCFSYNPGGLWTSEHQSSINGKFDNFLKEDLLAFAKIFGIKKP
                     KSILQEVIAAVSQWEKIARALEIPVERIEYINSHLRISNFD"
BASE COUNT         5534 a         3766 c         3660 g         5633 t
ORIGIN      
        1 tatttcttct tcgcggcaga cattttcatc gattggataa atcgattaac gttattgctt
       61 accatcacat tcgaatcgcc gccggcaaca aattgataag ttgaagtgcc atcaaaataa
      121 atatacatgc gatatatttt gcgattcatc aattgcattt caacatagcg accttcaatg
      181 ccattatttt caaccgattc aacttttaat gccttgccaa tatattgttt ctcggcattg
      241 gcaatcaatt catcgaagaa tgttttttta ttgctgaatt tatgccctgc gggatacgta
      301 cttactacta cttgatattc aattgcttcc tttaaatcag gagcgctgaa agtttgaacc
      361 tgaagtggtc cgcttggaag tgtataagtc tttgtcgatt ttgaaggctc catcggcaaa
      421 taacaactga atgaaggctg ttcaaaaaag aaccagttca ctaacacatt gcttgctgtt
      481 tggaatgaat ttagaaatgc ttctgcatat acagaataag aattatcgcc gggacctcca
      541 accaataatt gatataatac attatcctgc aataaaaatt gcgagcgcac aaaaaatgaa
      601 tcaccaacaa ctgcagtgaa ttcccttccc gtaaaattcg gattggaaac gatgcgttgt
      661 gaaattatct gaccgcttat ctgatcctgt gtgctttgaa taaattgatt aaagaatgca
      721 tcgcgatcat ctgctttaaa tgacggtgga tatactgtat atccaataga ataaaataca
      781 tcgctttttg cgtggttggt ctgataaaca aatttcttta tctcaccacc ttcaagccat
      841 ggagtagaag attcttccca gtcgcctttc acaggaactt ttacatgaaa gttcgcatcg
      901 gttccgcgca attcatacca gctctccata aataaagtat ccgccgattg catatcaaaa
      961 aaatcgccga agggatcgcg ataagcaaga taaccgggtt tttttccggg attgtccttg
     1021 atattgataa cctttattgt ttcaggatcg acatacccgc cggtcaccgg ttgaatggca
     1081 aatgacggga aatcatcggc aggaatattt gaagcggtgg cattgttgat ggtattattg
     1141 gtaagattcg gatccggttt cataccatcg gcatattttt tataatcaaa aggttcggcc
     1201 acaatggtgt aacctttagc ctgtaatttt ttgataattc caagatcgcc gccaatgaga
     1261 tcggcatcga gaatgaataa cgccgattgc tgttgcagca gtggatccat tttcttcatc
     1321 aacatgtcgt tcatgctgcc aatcattgtg gtgatatagg catcttgctc cgaagagttt
     1381 actacgcgga ggtagtcttt catcgattgc ccggcatagg cctgaaattt ttcattgtcg
     1441 gcgtctatat acaagctggc agtattgata tagttgctta atacggttgc ctgtgtattg
     1501 aaatccatca gatccataac acctgcaatc tggcgtatgg tcatgagcga taaagtaggt
     1561 ttcttctgtt gcagggctat gtcgagcagg atggtttgct gataatcaag accggctgca
     1621 tgatccttct cgcgcatcaa ttgcagggtg tacagtggtt tgtaggcgta ataagtttcg
     1681 atattctctt tcagtttatc ggcaaccatt agctcaaagc gaatcctgtc ctcacgtttg
     1741 taataccctt ttagcgtatt gtcgccggga gccttcatga acgtaggaat attctgaatc
     1801 tcagccgcat cgggcacatt aaagaatgct acggtattta ccttgctgat gataggtgtt
     1861 gcaggagtaa tgatatcgta gttctcgacg cccggaagct cggtagaaca aaggatgtac
     1921 gatgtaaact taccattagg gcttgtaacc ttccagagca gggactcctg cgcggaaagg
     1981 acattagtgg ccacagccaa cagtaacaga accaggatgc gaatcttcat gctattgctt
     2041 gattgggtaa atttaaggca aaaattgcgg tacctgctat aaatggatta cgtactgcag
     2101 catttcatga catggatatt tttgaaatta gtggcgaagg agccatagaa atcctgattc
     2161 gggtatttat tgtttacctc gtggtggtgc ttggcatgcg acttgtaggc aaacgggaga
     2221 ttgcgcagct gagtgtggtt gactttgtga tgatgctgtt gatcagcaat gccgtgcaga
     2281 atgccatgtt gggggataac acctctctct ccggcggaat cattgctgcc ctggccttgt
     2341 ttttgatcaa tttcgtgctg cggacactcg aatatcgcaa caaacgcctc aaagggttat
     2401 tggaaggcga acccgtaaca ttggtgtatc aggggcgatt gctggaagga aatcttaaaa
     2461 aagcacgtat aacggaggcc gaattaatgt cggctatacg tgaacacggg gtgaatgaga
     2521 tcggacaggt tgaccttggt gtgctggaaa taaacggaag catcagtatc ttgtcgaatg
     2581 atttttcgaa gcgcagcagt actgaaaaaa atgaagatgt caagaagtaa taacagaaat
     2641 atgcaacacc tttcttttgc taaaaatacc atcaccggca tcattttttt tgcatgtgta
     2701 caatttacaa atgcgcaatg cgtggttggc gattgtataa acggcaacgg cacctataaa
     2761 ttggaaaatg gtgatatgta taccggcaat tggataaatg gtgtgcgcga aggatatggc
     2821 agatatgatt gggcgagtgg cgcatattat gtcggtaatt ttaaaaataa tatgctcgat
     2881 ggcgaaggtg ctttttatgg tgtcgacgga acaaaaatga ttggcatttt tattaaaaac
     2941 aattatgcag gaactgctgc cgatacagca aaccggaaaa tttattcccc cgatgatgaa
     3001 atacaaaaag catataacga catcaaaaaa cgcgactcac tggcaaaggc aacaacgctt
     3061 gcaaaggccg agcgcgttga gttctgctcg ttgctgcgca ggctgacaac agattttcca
     3121 aatcaattca gcagctttct tggagaacgc caggaaatag tgttgagtaa tagtaaaaat
     3181 tggtatgcaa caattatggc taccggttca cttgaagccg gtgtgaatag tttttcagga
     3241 aaacctgagc gatcttatta taatatttta ttcgaaagtg ttgattccgt ttctgcgctt
     3301 gcacagtatg cacaatatgt tgaacaagtg aaaacttgca ataatggttg ttgtacgatg
     3361 gtgtatgatg aaagtacatt tcatgcaaaa tctcaatccg gttattcaac tatttgggcc
     3421 accttggcgg tgaaggaagg attggaaccg ctgttgtata ataatctgat aattgaaatt
     3481 gaatgcctca caaaaatggc tgaaccgggg tggatcgttg tgctcagaat gtacgatgcg
     3541 cagacgttga aatagggttt tgatggtggt tttctttttc tcactaagtc gcgaagtggg
     3601 taagacgcaa agttgctctc cttcgatttt ctgatttggt atacttttag ctctataaaa
     3661 gttaaaatat gtgacatgaa acagtttttc gctgctatta cattggtgtt ttttgtgcaa
     3721 ttggctgatg ctcagggatt tgtgtttgat tcggtgggtg tgcagcatat gaccattggc
     3781 aaggtgatgt taaaaagcga cacctgggtg atcagggttg aacgcggtgg cggttatgat
     3841 gatttctggc ctgtaaatct gccggaaaaa tatgccattg ccggtcagga agtggttttt
     3901 gaaggcgctt tgggaagaat tcccatgcag gcgcgacttg ttggcacacc ggtaaaactg
     3961 tcgatgatac gtgtattgta tcgcaccact ccgagagatg atgataaaag tggcgagtcc
     4021 attgcgaaag aaaaaccaat aaatcccgac tccgttggat atatcgaaaa gcaaacgggt
     4081 cgcattttga tggtgaatga tatgtacctg atagaaacta ccaccgttga tgcaaaagga
     4141 aatgaagaag tgaagcgcta cgtgccggat tttctgcctg acgatttcaa aatggcaggt
     4201 cagcaaatta cattcagcgc aataattctc aaacacgacc ctaatgtgcg tatgatggga
     4261 acacctgtga aaatcaaaga attgatgatg gaagaaaata cgcctttcga tgcaacacaa
     4321 ttgcaagaat cggttgcacc atattatcca ttcgattctg caggtcgact tgttgaaact
     4381 aaggcaatgg tgaaattaat tgcagatgta tatatacttg aagtgaccat cgacaatcaa
     4441 aataatatca cgcgatatat tccagccata cttcccgaat cgtttaaaaa agaaggaacc
     4501 attgtaatga tcagcggcac catcggaaaa ataccaccca atgtacgcat gatgggaacg
     4561 ccgttgacat tagaaagtat aaggttggtg gagtagttgg gttcatgcaa aatcgcaatg
     4621 ggcatgcgct tctcattatt cagtgtatta gcttctggaa ttcgtatcgg gtatcgggta
     4681 tcaggtatca ggtatcaggt atcaggtann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnntc tggaattcgt atcgggtatc gggtatcagg tatcaggtat caggtatcag
     4861 gtaaaaattc acgaatcacg attcacgatt ggtagtgaga ctaatgcaat tattcgctcg
     4921 cgttgcgagc gaatttgcat agtgaccact caccactcac cactcaccac tcacttcccc
     4981 tcagctattg tgattttcat tttcacatcc tcagcggggc gatcgccgta cattgttttt
     5041 actgcggcga ttttatcgat tacttcaagg ccttttacca cttcaccaaa aacggtgtaa
     5101 gagccgtcga ggaaaggtgt tccgccaatg gtttcatata ctttcttttg ctcaggcgaa
     5161 aagttcacac cataacctcg tgtgatatta ttcaattccg cttctgtcga gcgcttgccc
     5221 tgtacaatat aaaactgaga tcccgaactt gagcgcgatg gattcacctg gtcggcctga
     5281 cgagctgctg caagagcgcc ttttttatga tataatcccg gtttgatctc agcaggtatt
     5341 ttataatttg gtccgcctgc acctaattga acattgggtc ctgcattgcg ggaatccgga
     5401 tcgccacctt gaatcataaa atcttttatc acgcgatgaa ataacaggtc gttataataa
     5461 ccttctttta ccagcttgat aaaattatcg cggtgagccg gagtttcgtt atacaatttt
     5521 accagcatgt cgccataagt ggtgctgata tttacataat acactttgtt gtcgtccatt
     5581 gtatctgttg ttgttgtttg cgattgaatt tgttcgggag ttgtagttga gatcaccgta
     5641 tctgtttttt gcggaacgat gtcttctgtt gtttctgatt ttttttcact gcaattagaa
     5701 agtaaggcca cgcagcaaat ggcaaaaatg atctgtttca taataattta ttttcttcga
     5761 aatagcggat aatatgttgt ttgataatga tttcctgttc agcagccgac cacgccggta
     5821 attcttttgt cgcttcccac atgggccatt gatcatcgtc ccatcctgcc aggcgataat
     5881 agccgtcagc actcaacaat cggcatactg ctatatgcat gaggtcttgc ttttgttcct
     5941 tgctgaattt gtcttccacc ttacccaatt cgttcatccc aatcaagaat agaatagttt
     6001 ccagctcagg ttttttaccg aattggtcgc gcaaacgctg caataccgct atccaccggg
     6061 gatcaaaatc gataggtatc tcttcatgtg tcagcgacat gctgcaaaga tagggcatcc
     6121 tgatgtttgg aaggtatcag cggcgcacag gaatgcatat tcgagatgac agctatcttc
     6181 tcaaatcaca aagcaataag gaaactcaaa ccttgtctat aggagcattg tcgctatagt
     6241 aagtatcctc accaattcca tcagggtttg gaccatattt gtttgatccg tgttgtccta
     6301 aggggaagag gagataaatt gtataaaatg gaatgagcac ataccatccg ctgttaccca
     6361 gatcatgcgc gcgtttcaca ctctgtgcca gaaaaaacca aaccaagggt atatataaca
     6421 atagcagaaa caaggcgcct tgcgatagca tgttagcgat aatggcattc atgataattg
     6481 ctacaacaat atacaatccg aacgatttgc cgtattcggc tcggttgata cgtccttcaa
     6541 aagaaaatgg tgctgtgaac atgtggttgt tttttacaaa cataataaaa cattaccaaa
     6601 ttttaatcta aatcggaggt tcattttcaa aaagacgcaa acgtgcaaca gtcaatattg
     6661 catgatcaac aattttaata taacaatcag aaatatacgc gaaaacaaaa tatgtgtgaa
     6721 atccaaataa tattagtcgg aatcaaaatg tattaaactt caaaagtgtt atcaaaacaa
     6781 tagtttacag ttcaataaca gaagtgagtt ttaataataa cttttcgggc gatcgttttc
     6841 ccacagattt acacggagta ccatgccaat ggtattggcc tttccttcat tgctttcgta
     6901 tgcatcacct gaccatgttg ccgggttcag tgcaacattg acgtcccaaa aattctgttt
     6961 aaaaatcatc cactgataat atcctttgaa tgtaaatctg ccgccggcgt attgcagata
     7021 aggtgaaaca ccaataatga ggtcccaatt aatctcatag aaatcaccgg cgtcattgcc
     7081 tattgtatat gtgcgcgtga aattttttac agaaatagtg cttatatcaa acccgccata
     7141 taacccctgc accttatcct tctggccggt taattgataa gcatatccca tattaaacgt
     7201 attcatgcga tatttaaaat cgcgcgcatc ggaagttgaa gacgactcgg cagacacgtt
     7261 gctggtatgt cctataaatt caaattccat taaaaatttg gggaaatatg tttcaacggc
     7321 aacattccat ccgcgaaaaa aatgcacttc atccatgggc ttagatagat atgggcgagt
     7381 atcgttatat cggcctatta caaaattcag tccgtcgtta gtaatatggg tgccaagtcc
     7441 atatccaact cccatgttga atttcgcatt tgattgtgcg cgcaaaacat tgcatacaaa
     7501 taccaataaa ataaatacaa tgaaggttct catgttaatc aataaaatgt taagagtatc
     7561 ggtctacaac tcccgccgat ggtgtttgaa taatattaaa atccggctag aattttattg
     7621 taaaaatcga aggtcagaaa atacccatct ttaaaattgg tcataagcgt tacttcaatg
     7681 atcacagact catcaccctg aacattgatg actgcaccaa tgtcttgcat gagaaaccct
     7741 acatttgaat ataatgtttc tcctgaagca aaggccttgg ctgcgcccat agacgggaag
     7801 taataagcgt cggaatagat atcaggatac actttaacct gtctgattgc ttccaacaaa
     7861 gccgtatatg ccgcatagcc atcctgctca ttggcatagt cgcccaaata ggcataatac
     7921 cctaatgaag gatctatcgc acttaagtta ttttctactg cgcccgggaa ttgaagtgtt
     7981 gttgtgtagc cttctgaaaa gctcacatag gtgcccgtta ccaacttgaa actgtcacga
     8041 taagcctgat ataattcatt gataaattta tttacggcag acgcatcaaa agatagaggt
     8101 ccctgcctaa attcttctga tatcggagct gcaacctgtt tttgcggctc atattgttgt
     8161 cccgccacag gcaaaaaaga aaaataaaaa taatcctcat aggcgccatc cagattgaat
     8221 gttcctgcat accaaccttt agtatcaatg attgcctcgc tgaggagata gatagtgtct
     8281 ccaataaaat ttttcttttt cttaaagtgc gggtcttttt catttacatc tgcaatgatc
     8341 acaaatgttc cctttggaag tgtgtcgata cgcagtgtgg ttgcagtagt ctgggcagcg
     8401 ctgcgaaatc ctgaaaatag aaatgccatg actactaaaa gtgtggtggt gtgtttcaca
     8461 ataatgtaat atggatataa atctacttga aattcacaga ataccgtgaa caaggttcaa
     8521 aagagaattc tttacttcac atctaaattt caggcattat gaaaaacctt cagcacattc
     8581 ccaacattac gccacagcta aacgctgtaa gagcgcgact ccaacacacc caagatctca
     8641 tccaggcaag agctgcagcc cacgtattgc atccgaacga gtatcccttg ccggcagatg
     8701 gcaatagtct ggaacatcat tttgttgaag tctatcaact gctgtcagac aaaggcaaaa
     8761 aaaacttcga agaaaacgca aagctgatca tgggatatat gactgccgat cggagagcgg
     8821 aattgggtga cctagctaac ctgaatcaca aatcgccatt aagcactgtt gaacaagtga
     8881 aacaacttgg cactaatgat accatgcgta taaaacagga gcatgtaacg gcaaatgaaa
     8941 ccttactgcg taaacacctg ccaaaatcct ttccgggtat cgcttttgaa ggccctctaa
     9001 gagaagtaga tgtacgcggt cagaatcctc gcactttaag tttttttgtt gatagtattc
     9061 actgtgaaaa aacaacagac ttgcgaaagg atgagatatc attgtttggt tttacattcg
     9121 atgcatttgg acaaggaaca aaagttgaac aatttgaagt tggtaaattt aaaaaaggtg
     9181 aaaccattgc attaggcagc aaagctaaat tgagagattt cgatctgcgt gccccgggat
     9241 tttttccgca atcattcttc accaatttat ttttattcga aagggattgg gtgaattttg
     9301 aaaaaacagg taagcgcttg atgatgcttg gcgcggcatt gtcggcaaca ttcctgacgg
     9361 tgggattcta tttacttaca ctttcgccaa tcccggtatt gggaaccgca cttggtattg
     9421 caagtattgc ttgcttattg atgacagcta tttcgtatgg ttttgcgtta ttgattggcg
     9481 ccatgtgtga agacgtttcc ggtggggcag gtactgcatt tacttttaat atacctccgg
     9541 taataccggt aggaactgct ttcaccgaga cgctggattt tactattgaa ggcgggctat
     9601 tcggcgttca caaagcaaaa tattcagctc aggtgcgatg ggagcgcact gcttaaaaaa
     9661 aattcctaca acttcgttac tcgcatttcc actctgcgat ttttcgcacg gttggattcc
     9721 gtgtcattgg gagcaacggg tttatcggga ccaaaaccaa ccgccataat tcgcgattcg
     9781 ctgatgcctt tggaaacgat gtaatctttg cacgctttca cgcgcttata ggacaatgat
     9841 ttattcattg cataatcgcc ttcggaagaa gtgtgtccgc tcaattcaat ttcagctgat
     9901 ggatttgttt gcatgaaagc aacaattttt tccaactcgg ttttcgattc aggacgcaga
     9961 tctgcttttc cttgatcgaa taatatatta tttagcgtaa tactctgccc taccgtgata
    10021 ttattattat caaactttgc tgattttcct ttttccaaat aaataatgat aggatcattg
    10081 tcatcaccct gcccgatatc tgccgttgca cttccgtcga tataaccttc tgcggaggcg
    10141 ctcaccacat cgaatccggt aggaatatct gcaataataa attcgccgtt ggcatttgtt
    10201 ttggcaaagc tttgatttga agtaaatacc gttgcatttg caataggaat ctcagtatcc
    10261 tttatcatca ccacccctcg aacatttcct ttacaactgt tctcgcgctt tctatttaca
    10321 agcagtcgca cgaaatctaa tgcaaagcca tcgccaacac cattcacttc atcaatgctg
    10381 atcatacaat ttccgccatt agcaaaaagc ggataccaat cttcgggaaa aggaatagaa
    10441 attagttttc ccaccggccc tgtctgatca atggcgttca acattttttc tgcttcgaga
    10501 aaatgtttgc cgtttaccag aataatatac ttcgaacaaa atgaaggcgc ctgaaaatca
    10561 tcgatgaaca attgcagcca agcattttgc actgtagcat ctttcatttc attcatgggt
    10621 aaattccaca caacgggttt gtattgcatg gatgggtcag cgaagctata accttcagcg
    10681 ccgcagggaa agtcttgcat gccatgccat ttcgacgaca tcaatatcct gtcaaagccg
    10741 ggaagatcgt tggcttgggg ctcccaggga taggcatgtg aatccgtcat ccgtccgcaa
    10801 aaggggtcga aaccctccgg ccacccaaat ccgaggttat ctacatcgcc aagtcgaatg
    10861 atgtattccg cttccatggt gtttttgagg aaaacctctt gcttttccca ttctttatct
    10921 gaagaaacgg tggtcatttg ggcgaaaatg acattcgtca atattgttcc ggtaaccaac
    10981 agaagaaatg gcaacgcttt catttctaaa tgtatttgag atacaaggta agaaaaaaat
    11041 cacgccgcaa caatttcaat tctaatttta tggatactag gttttaacag actaaatcat
    11101 gtaaaaataa gatgattatt gaaagacaat ggtcaatagc attcacgtca atgataaaat
    11161 tcaagtaatt catgctatat ttaaatcatg aaaaccggaa ttatcttatt ccttatcatg
    11221 tacagtgggc tggtaaatgc acaaatgacc attaccacac tgcctgacga caatacagaa
    11281 cacgcaggta cctggttaca gtggccacat catttaacgt atggcactat ttatcgcgac
    11341 cgcatggagc cttgctttac ggcgatgacg gctgcacttg tgcctacgga gcatgttaac
    11401 ataatcgctt ataataacac agaaaaaaac aggatcatca gcgtgcttaa tgccaatgcg
    11461 gttccgctgg ccaatattac gttctacatc attcaaacaa atgattgctg ggcgagagat
    11521 aatggtccga tgtatgtgta tacaaatgcc gggcaattaa aaatattgga ctggggtttt
    11581 gatggttggg gaaacgatgc tccatataca aaagacaatg ctgttccgaa gaaaatctca
    11641 gatcaaaccg ctgttccgag aattaattta agcgcgatgg aacttgaagg aggagccgtg
    11701 tgtgtggatg gagccggtac ttttttagca acacgaagca gtattcaagg cgatggcaga
    11761 aatccggcgc ttactgaaac agatatcaat aattatttaa ttcaatatct cggtgtaagc
    11821 aatatcgttt ggcttgatgg attatatggc ggtggacttg atatcacaga tacgcatatt
    11881 gatggcttcg caacgtttct cgacagcacg acactggtta cattgagcaa ttcggatttg
    11941 agttattggg gaattacctc cgccgacaga accattttaa ataatatgac taatgcaaat
    12001 ggcaatgtgt atacaaaagt tattctgcca ctcaccaata aaaacgttaa aaccacctgg
    12061 ggtgcaaatg tcggtttcaa aggcagttat aataattatt acgtttgcaa cagcgtagtt
    12121 ctggtgccaa cttacagcga tgtaaatgac gccactgcat tgtccatcat ccagagtttg
    12181 tatccaagca aaacagttgt cggaattgat tgtcgcaatg catatttcta tggtggaatg
    12241 gtgcattgtt taacgcaaca acaacccgtt aatccaactc cacggatgga atattgcgca
    12301 tcaacagaaa ataatgcaca tttgacgata tatccaaacc cggcctctga taatattatt
    12361 gttcaaataa atagtaatga tggacagaca gtgcgaattt ctattttaga catgaatggc
    12421 aaaaccatgc ttgagatgcc ggcatacgca ttgcttgaag gcaacaatca aatcgatctt
    12481 caggtatcat ggcttccggc aggattatat atggtgcgaa tttccggtga tgttgaaacg
    12541 cagcggaatg gggtgtttca agttgtgcat taaagattta gcgcaatttc ttgtttaaat
    12601 agattatata aactttgcct aaatacaaaa caaaactgat tgaatattca atcatgtata
    12661 catcgtatta ttataaatta atcaagcagg aggaattgtt ttttattgca caatgcgaaa
    12721 tattttattg attatttcgg atttggtata agctttaaac tctcgcgtta acaaattgtg
    12781 ctgaccttct acattgacaa aaccaaacca ttcaaaaaaa cgatgaaatg ttcttagttc
    12841 gtagcaactt gcataaaaat gttcaggggt agaaaggcct ggttgaaaaa acaacacact
    12901 attaggaaaa gcgttcagaa atttctccga gtaaaaaatc gcctcttgcg gtttatcacc
    12961 atacttatcc aacagaatta ctgaaaaagc ccatccaaat tgtgccacag ggtcttctcc
    13021 atatccatca ttacaactcc aggcaaattt atttgtaaag gcttgcataa ataaatgaaa
    13081 gaacttattc cggtcatact tattcagcag ttttattcct gcttttgtga gggttaattt
    13141 tccatttgtt ctctttacca atccggtcag ctccacggtt agtctggcac tcaaaattgc
    13201 agcacaatct tgttctcgtc gcaactttac ataccctttt tcaatgaagt cttcaagaat
    13261 atacccataa ttatatagtt ctgtcaaaac ttttactggc agagaaccac caagtgttaa
    13321 ttttattcca ttttctcttt gaataatatg caaaaaaacc tccgcaatac ggaatatagg
    13381 tatttgatcc aatattgcat catctattgt gtcaaccatt tgcagagaag attctttgtc
    13441 atatacatta tataacaaac aatgcatttc actaggggtg agtccttcaa acgcctcaat
    13501 cggtgtatta ttctgcaaat gcttgattgc ttctaaatcc tctttcataa ccactcaatt
    13561 acgcctaatc aaaatacgtt taacaattta ggtttctaaa ttaagtatat tttttgattg
    13621 catttcttta cgaacaatta atatacgtgc cccgtttttc acgcggatcc cgatagctat
    13681 cgggagcgga ggagcggaga aatacaagtt tttcacgcag agagcggaga aatacaaaag
    13741 ttaattgttt ttattattcc gcattgttca ttccttattc ttcccctcac ttctcacgtt
    13801 cctcacccca accatccctg gtgcacggcg aataacacta ttcctgtgcg ggattttaaa
    13861 tcgagtttgg agaacaccga ttcgcggtag ccatcaacgg tgcgttggct gacggacatt
    13921 ttgtctgcta tttctttgta ggtgagttcg gagcagcagt gtttgatcaa ttctttttcg
    13981 cgctttgcta ggttgaactt gatattaaat ccctttgatt tattccaaac taattattca
    14041 taaatgtaag gtcaattatc atgcacgtgc ttgtatatac aatttcctaa ttcatttcat
    14101 tcatcaccac atcaaaatca tccaccaaca cctttagttt acgttctgcg gactttacgc
    14161 gcattctgaa gtggcggttc gcgcgcatac ggcgtgcata aataaagtat actaatgcaa
    14221 tgaggagaca aaatatcaat acagaatatt ttattaaagt agcatagtta tttgcatcag
    14281 gattttcact ttcttttgat aatttttcgg caatattaaa ttctgaactt aaatatccgg
    14341 ttaaataaaa tcccaaaaaa gtaaaaatca gtgtaaacaa ataagccaac cattctgaaa
    14401 agtaaacaaa tgcttttcga gtatagtata cgtagtcatt ttgtcctatg aggtctaata
    14461 cctccttgag tttggttttc atggcattta ttttattgtg tgaatttaat acttatttgc
    14521 tttttaatta acaaaatatt tccgcatatt gggtgcataa aacctattgg ataaagaaaa
    14581 accgcatatc cacgctcgta atgccgcatc gggttccgaa taaacagatt cttaatttaa
    14641 ccccaactgt ccataccttt tcaccaggag ttctatatac atggttttca tgtctggcga
    14701 caaaaacgat ttatcaatcc attcaatcgc cttcgagtta ttttccagca ttcgggcaaa
    14761 acttccgttg atctgctttg gtgttaatcc cagacttgct cccagttgtt caaaatgctc
    14821 tctcattaat tttcttttct tgccggccag ggttagggca agttcttcct tgtcctcagg
    14881 aagtataatc gcaacattta ataaatcata agccggcgac aacacccagc ctgaagggcc
    14941 ttctatcatg gagaaatttt tcaggtgcat gtcattgttg ccggttaaat aggaaaaaat
    15001 tgctagatcc aaataatatg tgcaatccag caagggatta cttgaatatt gattcaaggc
    15061 tttgccaact ttttccattg aacttcgata cttatcaaac gcttcagtaa tttgaaacat
    15121 atctatcatg tgtatcttcg ttcctgcgac agtgcggtcg gcacgctttg tgatatatga
    15181 gagctcccct gattgtaatc gaatcagcga tgattgcatt acctggatgc caaaagattc
    15241 agcaatacgc atcgttgcgt gttcattttc cggcatctca ggatagcggt cagatggtgg
    15301 tttgaaaata tattggccac ccaaagcatc aacaactgta agacgaacag ctttctcttc
    15361 ctttgttttt tttatcagcg acatggatag tttagcctga acacccggaa cggccacact
    15421 tctctctacc acctgttttg caagttcatc catttgatca agtgaatatg gtatttcagg
    15481 aggtacagat gttccgaaaa actgcatgga acacttctcg tgaaaatcat tttcactttc
    15541 aagagattcg taacaataca ggcatttatt tttcatcatc aaccataatt tgttctacac
    15601 ttactgcacc aatgcaattt ttgcaacatg ctaacaataa tcccatgcga tcatttgttt
    15661 gtattctcca attttctgca gcaatattca ataaccaacc ttcgggaatc aatccttcaa
    15721 aaaatggaaa tagtcgcttt tctgtatatg gctgattgcg cacaggcata gtaaatgtta
    15781 taaaatcatc aggatgatcg atcacataag cctcatcata ctgaaataca aattcacctt
    15841 catttgtttc ggtgataatg ccggctaaat gatctttata aaacaccttt cctcttctca
    15901 tacatcaata tctttaattt taaccggagc cagttcatgt ccgaacattc taagcaccag
    15961 atttacctta tccatattca ggttcgtttt tccctgctcg attttacgga taacggtgag
    16021 tgcaacaccc gagcgttcag caaattcttc ttgcgtcagg ttgacctcct ttctccgctc
    16081 ctttacaaac tctgataact gtttcattat atgcatttag atataatacc atacaaatgt
    16141 agcaaattat atgcaattaa atataatgaa taaactttta tcataattta tatgcattta
    16201 gatataaata gtgagcaaac aatctacttt atatgcattt acatataatt cattgtccaa
    16261 aatgcattaa aacgcctgtg ttgcttgcgc cggaggtaga aggtaggggg gttccaggcc
    16321 ttgttcctca ttgaagaacc agtgataggc gatatatata ttatgctact ctagcttgcg
    16381 tactacatca gaatgattgc caatcctgtt cttttggtac catattttgt tgctgttgaa
    16441 atctcaggct tatagaccta aggaaatgtc tcagccatat tattatctta gattcttgtt
    16501 catacatcaa caaaataaaa agacactcaa atgcctttcg caactgtaaa aaagaaattt
    16561 caaaaactag ataaaaaaca attagttgac ctgattggta atttgtataa actaaacaaa
    16621 tcaaatcagg agtacctcga tcactttgca gaaccagacg aagctgcgct ctttcataaa
    16681 tacaaagaac aaattattgc agctttttat ccgcctggta accggcctat cgacattcca
    16741 aatgcccgca aagcactcat ggcttttcaa aaactgcatc cctcgccgga atatgccgca
    16801 tcactcctgc ttgtctatgt tgaaactggt atttcttttt gtaacgactt tggatacgtc
    16861 gacgacccca tattgaatag tttaagtgta aaatttgaag atgccctgaa atttatcgaa
    16921 aaagaaaata tgattgatac tttcaaatcc aaagcgtttc aaattacaca tgatgcatca
    16981 gacatataca ccccgatcag cgactatttt gccgatcttt gtcaatatta tttaggctct
    17041 aatccccatg atccggaata attttgtttt gatgccgtct gtatgtccac ttactacgca
    17101 ttacgaatat tttcatttta gttcaagttt aatattgact gcttacacaa gaatccaatc
    17161 tatagaaaat taagtatctt tattttatgc tcaaaatgct ttctcttttt tggtgtttga
    17221 tgaccatcac gctttgcttt gcacaagatt gcaaacttaa taaatcactg aaagtgccca
    17281 caaacaggca agtttacatg cttggcgatt ttcatctcga cgccaaacaa tatattcatg
    17341 ccgaagattc taccattaaa aacaccattg ctgccgaaga tgctttacgc aattatctgg
    17401 tcgacaaatg tgatgtgaaa catttcttta tggaatccag tgtcagcttt gaatattttt
    17461 tcaataagta ttttgaaacc ggagacacag gatggatatt tattttcaac caacagaaat
    17521 atgaaattgc aaaattagag tcgcttcgaa gtatttatgt aaagcatccg gatatttcag
    17581 taaactgtat tgatgtagtg caggagtctt atgtaccgca gatgttgatg actttatatg
    17641 cattaagctt ctatgaacat tttcaagaat attacactcc accgctgaat aacacaccgc
    17701 tattcccgcc atggaatgat ataaacgtcg gcattcgact actcgacagc ctgcctcaat
    17761 gtaacgacac tataacgcca tttctaggtg caataattca atcattgatt gcaccggatg
    17821 tcaatacaaa ttacaatctc tattatttgt ttcggctcac gttacaaagt gctcaggcgc
    17881 aagaagcttt gcagaaattt tatggtgatg attacagcta tgtgatgcgt atcatacgcg
    17941 gatatgtttt tggttatggt aaatcactgg atgacattct cttcaaatat cggcagcagc
    18001 gagaagatgt tattgaagag accgtaaaac aattattggt cgataataat tccggagcgt
    18061 atttaattca aatgggcaat gcacatctcg acgccgactc cactgccaat atgttgcgct
    18121 accgacttat gcaaaacaat atcacagcgc cattctgttt ttataatgtg caatcaatgg
    18181 ctaaagattt ctttgaaaaa acttttcata cgacaccgct tgatttttcc ttttcgtcaa
    18241 gttattgctt tcatcgcgga ccgggggata atgtgtatgt gttagtgaaa taagcggatt
    18301 ggtagcgctc tgacacttcc ttattatagc cagggtaatg gatttaatat aaaatcaatt
    18361 attatggctg ctccgccgtt ttattttact atagttaaac cggcttgagc aattgtacta
    18421 tccgccacct atttttaatc gaaatttgat attcttaaat ggctattaat atattcaatc
    18481 ctttcaacgg gaatttctaa tgctcttgca attttttccc attgactaac tgcagctatg
    18541 acttcctgca aaatgctttt aggttttttt ataccaaata ttttagcaaa cgcaaggagg
    18601 tcttctttca aaaaattatc gaatttacca ttaatagagg attgatgttc gctcgtccat
    18661 aagccgcccg gattatagga aaaacaaagg tca
//
LOCUS       DAOQHY010000050        18411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1390_length_18411_cov_5.219983, whole genome shotgun sequence.
ACCESSION   DAOQHY010000050 DAOQHY010000000
VERSION     DAOQHY010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 18411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18411
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1390_length_18411_cov_5.219983"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..898
                     /locus_tag="PLL28_05860"
     CDS             74..898
                     /locus_tag="PLL28_05860"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNF68882.1"
                     /translation="MDSLLDRLNEAVHFIRQRTDFQPEFGIILGTGLGNLVSHIDIVA
                     EINYHLIPHFQVSTVESHSGKLIFGSIGDKKVVAMQGRFHYYEGYTMEEVVFPVRTMK
                     LLGIKRLFISNAAGSLNSNIEKGDLMILRDHINLQPSNPLIGPNEYDLGPRFPDLFRC
                     YDENLIETAMSIARDNNIRCHKGVYVSVPGPNLETPAEYNYLHIIGGDAVGMSTVPEV
                     LAAVHMGLPVFAISVITDKGYPMEEIEPVTVDDVIAVAKEAEPKMTLILQKLIAQL"
     gene            1086..2849
                     /locus_tag="PLL28_05865"
     CDS             1086..2849
                     /locus_tag="PLL28_05865"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68883.1"
                     /translation="MQTWVEPDTTLDNFAMFNPARQDLFENAWLGNLGSPYTPRWFDY
                     HRELGFDYGRHERDAYITTLDEVKYYRTNVPFTRLYYLAGQNEEQLFHVTHTRNFGPQ
                     GNVAIDFSKLVSTGYYQYSRNDYGNTTLSGWYTTKNNRYLIEGAAIRGKNNNEENGGI
                     NNDTLFNYPNPELAEPFRTQAFTEWSNWQGRITQSYQLGKNFSYQINDSTTGNYFDPK
                     FVITHTLTGIDHYYLFEDPATDRTYYGTLLYESDTLRDRTSVRGFENKLQFATSLTHR
                     VSVDSVIPAKLFAEIYALQQHHILSDQAGKQDYDHFIIGTHLLSPSVGKHGGRLGIDA
                     AYDLINKNYDTKLIIQFDSINPESHIFVRYGLLNPTVIESHYSGFRDEWNGSGMPTVF
                     SLGLNAESKPQHFTFHASFSMFKDYNYASTSTWFEEHYLHTNTLDFNIGQAYIRKKIT
                     LGNFVFDNVAGAQFAMDITSIPNFIYTGDWYVQGNLFKNALYSQAGIHFWGSSAYTPM
                     KYDMITGRFLEFASYGTNNSSTFPPVIDLFASFDIRTFRFFVRMDNIAQGLFNKGYYE
                     APNYPMQPRSFKLGINWDLFY"
     gene            complement(3027..4283)
                     /locus_tag="PLL28_05870"
     CDS             complement(3027..4283)
                     /locus_tag="PLL28_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68884.1"
                     /translation="MKRYLIISLFLISGLAAKASYILLPMDNTQKDHLKCYGIAYWLL
                     ENGGKVDWMLNYRGGSFCFQYLDVYEKECLIRGVSYEVIPDASYNKIVADIADPEVNM
                     DVMKLEKAPKMAVYSPKTKQPWDDAVTLVLTYAEIPYTVIYDQEVMDGELPKYDWLHL
                     HHEDFTGQYGKFWSAYHTQAWYQQQEQENEAIAKEYGFTKVSQLKLAVAQKIRDFVSG
                     GGFLFAMCSAADSYDIALAAEGVDICDVMFDGDPADPNAQQKLDFSKTFAFENFTISR
                     NPYEYSLSTIDVSHSRSVPKELDFFTLFDFSAKWDPVPTMLTQDHEKTIPGFYGQTTA
                     FKKDLIKSSVLIMGENKAANEARYIHGEYGYGTWTFYGGHDPEDYKHYVGDPPTELEL
                     HPNSPGYRLILNNVLFPAAKKQKQKT"
     gene            4598..5302
                     /locus_tag="PLL28_05875"
     CDS             4598..5302
                     /locus_tag="PLL28_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HNF68885.1"
                     /translation="MNWFSTFLTSSIGRKVVMAVTGLFLISFLIVHCTINAMIFFNDG
                     GESFNKAAHFMGTNVIIRTMEIVLFLGIILHIVQGFMLSAKNRAARPTGYAVTNPGAN
                     SKWYSRSMTLLGTLILLFLVMHISHFWTHSRLGGIGGISPLDETMIGDKEVLNLYALM
                     ISTFQSPVVLIVYILGVISLCWHLVHGFQSAFQTLGVNHKKYTPAIKGIGIVFSILIC
                     ALFAAMPLSIYMGWIA"
     gene            5383..7314
                     /locus_tag="PLL28_05880"
     CDS             5383..7314
                     /locus_tag="PLL28_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HNF68886.1"
                     /translation="MLDSKIPAGPLSEKWTTYKNTVKLVNPSNKRKLEIIIVGTGLAG
                     ASAAATLGELGYKVKVFCFQDSPRRAHSIAAQGGINAAKNYKNDGDSTYRLFYDTIKG
                     GDFRAREGNVYRLAEVSANIIDQCVAQGVPFAREYGGLLDNRSFGGAQVSRTFYARGQ
                     TGQQLLIGAYQGLERQVGLGNVQEFSRHEMLDIVTIDGKARGIIARNLVTGELERHFG
                     HAVLLCTGGYGNVFYLSTNAMGSNVTAAWKAYKRGAFFGNPCYTQIHPTCIPVSGDYQ
                     SKLTLMSESLRNDGRVWVPKSREDAKAIREGRKKPTDIAEEDRDYYLERKYPSYGNLA
                     PRDISSRAAKEACDDQRGVGESGLAVYLDFSAAINRLGKHVIEERYGNLFDMYAHITA
                     ENPYEVPMRIYPAVHYTMGGLWVDYELMTTVPGLYALGECNFSDHGANRLGASALMQG
                     LADGYFVIPYTIGSYLAGEIFTKAIPTDHPAFEETEKHVRESLDKLLSIQGTKSVDHY
                     HKELGKIMWDYCGMARTAEGLKQAREMVKKLRAEFWQNVRVTGKANEFNQDLEKAMRV
                     ADFIELGELMIVDAYERNESCGGHFRTEFQEEGEAKRNDDEYAYVAAWEFRGVDNDPA
                     MHKEELKYETVKLATRSYK"
     gene            7381..8139
                     /locus_tag="PLL28_05885"
     CDS             7381..8139
                     /locus_tag="PLL28_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HNF68887.1"
                     /translation="MNVKLRIWRQRNAQEQGKFVDYAVNDANPNMSFLEMIDVLNESL
                     VNKGEEPVAFDHDCREGICGMCSMYINGRAHGPQKLSTTCQLYMRNFSDGETITIEPF
                     RAKGFPVIRDLVVDRSAFDRIIAAGGFISASTGQAQDANAIPISKTNADLAMDAAACI
                     GCGACVAACKNASAMLFVSAKVSHLALLPQGDPERRKRALAMVAQMDAEGFGSCTNTY
                     ACEAECPKGISVANIARLNREYLNAAIVSEEMPK"
     gene            8319..8633
                     /locus_tag="PLL28_05890"
     CDS             8319..8633
                     /locus_tag="PLL28_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNF68888.1"
                     /translation="MRRDIFQAIADPTRRAIIALVALHAMTPNAIAENFDITRQSVSK
                     HLRILTECELLKQTPNGREIYYSLEVSKMKEVDLWLEQFRKLWEGQFAQLDQLLEQMK
                     KQ"
     gene            8635..9129
                     /locus_tag="PLL28_05895"
     CDS             8635..9129
                     /locus_tag="PLL28_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNF68889.1"
                     /translation="MADNLPLELTIDKASKTVTITRAFNASQDMVWDAFTRSEFLDQW
                     NAPKPFTARTKYMDFSVGGKRFYAMVSPDGQERWILQTYLVINPKTHFKYFNVFADAN
                     ENPDQFGSEWDISFSEQAGLTTVQISIFNESLERLERMLELGFKEGYAASMANLEALL
                     ENMQ"
     gene            9186..9974
                     /locus_tag="PLL28_05900"
     CDS             9186..9974
                     /locus_tag="PLL28_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNF68890.1"
                     /translation="MKQKIYQVDAFAEKLFSGNPAAVCPLDQWPEDSLMQQIAMENNL
                     AETAFYVNNGDYYALRWFTPTVEVDLCGHATLAAAFVLWNYEGHSSDEIKFISQRSGE
                     LKVKRSGAMMTLNFPTDTLSEIGVSDDIKACFNISPLSAFKGKTDYMLIFNNESEITN
                     IIPHLDQINKLIARGVIITAPGDVCDFVSRFFAPQAGVPEDPVTGSAHTSLTPYWSKQ
                     LGKTELSAIQLSARKGYLTCKYLGDRVEISGKAVLYLVGEINVK"
     gene            9988..10407
                     /locus_tag="PLL28_05905"
     CDS             9988..10407
                     /locus_tag="PLL28_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNF68891.1"
                     /translation="MSIETQIQQYISSQPEAKRNDLLALHKSILYIKPKAKLWFLDGK
                     NEAGKVVSNPNIGYGLLQLKYADGSAKDFYQVGLSANTSGISVYIMGMADKKYLADTY
                     AQAIGKATITGYCIKFKALKDINMDTLQHAIKDGFAA"
     gene            10490..11083
                     /locus_tag="PLL28_05910"
     CDS             10490..11083
                     /locus_tag="PLL28_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68892.1"
                     /translation="MTEETSTEVQQTDYQGISVEELLKKAMLQDTMKFDRWEPFISFV
                     SYRSQENKLYAFAITRHTDSTMLISRYALQDSTWKLISSLSGLRGPGVFYHLNVADYD
                     FDGVRDVFITSVTSNGIALSQGNLVITDDIDNILVYHPEADTLYSPIPDPNRKLVMSQ
                     KVINCAETGFRNVCDITNKWVDGRLVTTEQNCPCETE"
     gene            11369..14104
                     /locus_tag="PLL28_05915"
     CDS             11369..14104
                     /locus_tag="PLL28_05915"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNF68893.1"
                     /translation="MLKKRVCKALIFMLLVVVSAPKAWATHNRAGEITYTHVEDLTYE
                     FTITTYTDPASLADRDRLGIDWGDGTVDTIMRNSIELLTPDIQKNQYISTHTFAGPGI
                     FIISMVDPNRVESILNIDGSVNVPFCLVDTFIIFDPLTLGYNNSPLLLYPPIDYANIG
                     EFFIHNPGAYDIDGDSLAYKLVVPFQYPGIVVPGYLDPDDRTFCTDTDVFQINTFNGD
                     VTWEDPSCTEGIYNIAILITEYRNGIKMGTILRDMQIFVNKTSNHAPEIAELNDTCVY
                     AGEELNILVTATDPDVLQTITLTATGGPLYIDESPAEFFSTPNEGSVNGTFTWQTICN
                     HVRKEVYEVVFKAEDDFQIGFEEIPLVDLETWLITVVAPPPVILDAVPTANEITVTWQ
                     DPYICSGADNFLGFSVWRKIGCDSTMFDKCQRGLSGMGYDSLAFVEDAYSYVDLAVDY
                     GQEYAYRIVAEFGERSDIAPDFVYNQVPGAPSDAVCAELKRDIPIINHASVRTTSTTN
                     GSMYVGWYNPISPDLDTTIFTGPYKYELVRYEGFTPTGTETLVTTITAGEFFEITDTS
                     YIDTMLNTAATAYCYRLKFYFTDLGNFELLGVTDPASSVYLTASPGDNKLNLSWNFNV
                     PWLNFYYDVYKETTAGSGDFELLTSTTETSYIDDSLANGSEYCYYVKAYGKYTIESLP
                     DTLINLSQIQCGVPIDNEPPCPPILTVYNICEEDGSIVVDESDLKNDLTWTNPNHSCA
                     DDVVKYLIYYASQQGDPLTLLDQNADAFDTTYTHDGLLSLAGCYAVVAVDSFDNNSAF
                     SNIVCVDNCSDYNLPNTFTPNGDGFNDIFTPILPYRFIDHVDMKIFDRWGNVVFTTND
                     PMLNWDGIDTNTGKNASEGVYFYTCTVYEITVDGVRPFPQPKEGYIHLYRESKVE"
     gene            14178..14762
                     /locus_tag="PLL28_05920"
     CDS             14178..14762
                     /locus_tag="PLL28_05920"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNF68894.1"
                     /translation="MFTGIIESLGSVSGLQKDAGNLHITVQSEISRDLKVDQSVSHNG
                     VCLTVVHQAGDTHTVTAIDETLIRSNLGNLKIGDTVNLERCLKIDGRLDGHIVQGHVD
                     TTAQLIQVDEQGGSWLLTFRFDAQFDKYIVQKGSISINGISLTVFNVSEGTFQVAIIP
                     YTWEHTNLSQISVSDSVNIEFDIIGKYVEKMMAR"
     gene            complement(14811..16676)
                     /gene="hscA"
                     /locus_tag="PLL28_05925"
     CDS             complement(14811..16676)
                     /gene="hscA"
                     /locus_tag="PLL28_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HNF68895.1"
                     /translation="MAQISINIKEGTLKREEIIVGIDLGTTNSLIAIIDKETGTPSAL
                     RDADKFAIVPSIIHFADDGTITVGRSAREHLITDPEHTIFSVKRLMGKSYKDIAAYQH
                     TFGYKIIDDDAERLVKIKVREKYYSPIELSALILKELKLRAERLLQCAITKAVITVPA
                     YFNDAQRQATRDAGKLAGLDVLRIINEPTAASLAYGLGNQDKSSHTIAVYDLGGGTFD
                     ISILRITSSENSGDFFEVLSTNGDTFLGGDDIDRAIMDHWLQTQHVDGNLLSATVRQD
                     LRLTAETAKKHLGSHGEFTGNVDKIQCDLTIAELETLITPLLQKTIQCCERALFDANL
                     NVNDIDTVVMVGGSTRTPFIKKSVSAFFNKPVHDEVDPDEVVALGAAVQADILAGNQK
                     DMLLLDITPLSLGIETMGGLMDVIIPRNTKIPTAAGRQYTTSLDGQANLKISVFQGER
                     DLVRDNRKLAEFILSNIPAMPAGLPKIEIVFQINADGILQVRAKELRSGVEQKIEVTP
                     QYGLTEEEMAAMLLDSIKNAKTDMAERAIVEARVEAQNMIDTCERFIEKNNQYLNEQE
                     IAGTKSLIDNLRNTLNSDDKDVIHQQIEILNEFTRPFAERLMDMAISQAMKGKTI"
     gene            16892..17929
                     /locus_tag="PLL28_05930"
     CDS             16892..17929
                     /locus_tag="PLL28_05930"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNF68896.1"
                     /translation="MYKTLLKPLFFRFQPETAHHLALGLYKAALWIPGYGWWHDKHYV
                     IERPELEKEVFGIRFKNSIGLAAGFDKNAAFIPLMARLGFGHVEVGTVTPKAQPGNDK
                     PRLFRLPKDEALINRMGFNNEGVEMMVRNLGKSSVAHAREKYRVVIGGNIGKNKVTEN
                     EDAWKDYVICFNALFDHVDYFVINVSSPNTPGLRALQEKEPLKRIISSLQEINQGKKR
                     PKPLLLKIAPDLNDAQLNDIVEITRETGLSGIIATNTTLDRSMLKTPQAAVESIGAGG
                     LSGKPVLSRSNDVLWFLRRALGTDVPLIGVGGVTDKASMQSKFDRGADLVQIYTGMIY
                     EGQGMVRGVLV"
     gene            complement(18120..>18411)
                     /locus_tag="PLL28_05935"
     CDS             complement(18120..>18411)
                     /locus_tag="PLL28_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68897.1"
                     /translation="MHRFWNAGDDVLKCEGWISPPNNIIYYLENIYRLQNEGGGEPGG
                     FEAAYLIRKYRSEFDVAAIPGFVKNVIFPIVVFFGKLAGKYRKFADAPPAVR"
BASE COUNT         5291 a         3684 c         4110 g         5326 t
ORIGIN      
        1 gcacgccttc ccctacagaa ccaacagtat caaacgaata aatgaaccct tatctttgta
       61 aatattttca attatggact ctttattgga tagattgaat gaggcggtgc actttatccg
      121 ccagcgcaca gattttcaac cggaatttgg tatcatcctg ggaacaggat taggaaattt
      181 agtttcgcat atcgatatcg ttgctgaaat aaactatcac ctcatcccac attttcaggt
      241 atcgacggtt gaaagtcaca gcggtaaatt aatttttgga agtataggcg ataaaaaagt
      301 agtggccatg cagggacgct tccattatta tgaaggatat accatggaag aagtggtatt
      361 ccctgtgcgc acgatgaaat tactgggcat caaacgtttg tttatttcga atgcagcagg
      421 gagcctcaat tcaaatatcg aaaaaggtga tttaatgata ttgcgcgatc atatcaacct
      481 gcagccatca aatcctttga tcggtccgaa tgaatacgac ctcggtccga gattccctga
      541 tcttttccga tgctacgatg aaaatttaat cgaaacggcc atgtcaattg cgcgcgataa
      601 caatattcga tgtcataaag gcgtatatgt aagtgtgccc ggaccaaatt tggagacacc
      661 tgcggaatac aattatttgc acatcattgg cggtgatgcg gttggtatga gcaccgtgcc
      721 cgaagtgttg gcagcagtgc acatggggct gcccgtattt gccatttccg tgatcaccga
      781 caagggatat cctatggaag aaatagaacc tgtgactgtt gatgatgtga tagcggtggc
      841 aaaggaagca gagccgaaaa tgacattaat tctgcaaaaa ttgatagcgc aattataatt
      901 tggggaagaa atcgaaattc cattttctca gttcaatcgt caggcgtatc tccgcaacaa
      961 cgattctgct gttgtgtaca ttagcggcat tcgcccaaac tgattcgctt gcgggcgaca
     1021 gcacgtcgtt acccagttta tacaactatc acaacgcccg cgtccaattt cataccgtcg
     1081 cggaaatgca aacttgggta gaaccggata ctacactcga taatttcgca atgtttaatc
     1141 ctgccagaca agatttattt gaaaatgcct ggcttggaaa tttaggatca ccatatacgc
     1201 cgcggtggtt tgattatcat cgcgaattgg gatttgacta cggtcgccat gaacgcgatg
     1261 cctatatcac cacactcgat gaagtaaaat attatcgcac aaacgttcct ttcacacggt
     1321 tatattatct agccggccaa aatgaagagc aattatttca tgtaacacat acacgcaatt
     1381 ttggtccgca gggaaatgta gccatcgatt tttcgaaatt agtgtctaca ggatattatc
     1441 aatattcgcg caatgattat ggaaatacta ccttgtcggg atggtatacc accaagaata
     1501 accgatattt aatcgaaggc gcagcaatta gagggaaaaa taataatgag gagaatggtg
     1561 gtatcaataa tgatacctta ttcaactatc ccaacccgga acttgcagag ccatttcgca
     1621 cgcaggcatt tactgagtgg agtaattggc agggacgtat tacgcaatca tatcaactcg
     1681 ggaaaaattt cagctatcag atcaatgatt ccaccacagg aaattatttc gaccccaaat
     1741 ttgtcatcac acatacctta accggaatag atcattatta tttatttgaa gatcctgcaa
     1801 cggacaggac atactacggc acattattat atgagagcga caccttgcgc gacagaacat
     1861 cggtaagagg atttgaaaat aaattgcaat tcgcaacctc gcttactcat agagtaagtg
     1921 ttgatagtgt aattcccgca aaactgtttg cagaaattta tgcattgcaa cagcatcata
     1981 tcctgtccga tcaggccggc aaacaggatt acgaccattt cattattggc acacatttgc
     2041 tgtcgccctc agttggtaaa cacggcggac gacttggtat tgacgctgca tatgatctga
     2101 tcaataaaaa ttacgacact aaattgatca ttcaattcga ctccattaat cctgagtcgc
     2161 atatttttgt tcgctacgga ttactgaatc caacagtaat tgaatcacat tattccggat
     2221 tcagagatga atggaatggt tcaggtatgc caaccgtatt ttcgctagga ttaaatgccg
     2281 aatcaaaacc acagcatttc acctttcatg cctcgtttag catgtttaag gattacaatt
     2341 atgcatcaac gtctacatgg tttgaggagc attatttgca caccaatacg ctcgatttta
     2401 atatcggaca ggcatatatc cggaaaaaaa tcacattggg caattttgtt tttgataatg
     2461 ttgccggtgc gcaatttgca atggatatta cttccattcc gaattttatt tataccggcg
     2521 attggtatgt tcaaggcaac ttatttaaaa atgcattgta ttcacaagcg ggtattcatt
     2581 tctggggttc atcggcatat acaccaatga aatatgatat gatcacaggt cgcttcctgg
     2641 aatttgcttc ctatggcacc aataatagca gcacttttcc tcctgtcatc gacctcttcg
     2701 ccagcttcga catccgcacc ttccgcttct tcgtgcgcat ggacaacatc gcccaaggtc
     2761 tcttcaacaa aggctactac gaagccccga attaccccat gcaaccgaga tcgtttaaac
     2821 ttgggattaa ttgggatctt ttttattaaa tggtatcagg tagcaagtat caggtagcag
     2881 gtatttgtag tgagtagtga gtagtgggta gtgggacgga aaattagatt ttggaattta
     2941 ttgcattgag tgctcctaaa ttttaaatgc aaattaattt ctctgcgcct ctgcgtctct
     3001 gcgtgaaaaa aaacacgaca ttaccatcac gttttttgtt tttgtttttt tgcggcaggg
     3061 aaaaggacgt tatttaggat taatcgatag ccgggagaat ttggatgcaa ttctaattca
     3121 gttggaggat ctcctacata gtgcttgtag tcttcaggat cgtggccgcc gtagaaggtc
     3181 caggtgccgt agccatattc accgtggata tagcgcgctt cattggctgc tttattttca
     3241 cccatgatga gcacgctact tttaataaga tcttttttga atgcagtggt ctgaccgtag
     3301 aatcccggaa ttgttttttc atgatcttgt gtcagcatgg ttggaaccgg atcccatttt
     3361 gctgaaaaat caaataaggt gaaaaaatct aactctttgg gaacagaacg cgaatgagaa
     3421 acatcaatcg tgcttaaaga atattcgtaa gggttccggc tgatagtaaa attctcgaaa
     3481 gcgaaagttt ttgaaaaatc aagtttctgt tgcgcatttg gatcagcagg atcgccatcg
     3541 aacatcacat cgcaaatatc tacaccttca gcagcgagtg caatatcata actgtcggca
     3601 gccgaacaca tcgcaaataa aaatccacca ccgctgacaa aatctctgat cttttgagca
     3661 acagctaatt ttaactgcga cactttagta aacccatatt cttttgcaat ggcctcattt
     3721 tcctgttcct gttgctgata ccatgcctgt gtatgataag cgctccagaa tttaccatac
     3781 tgacctgtaa aatcttcatg gtgtaaatgc aaccaatcgt attttggtaa ttcaccatcc
     3841 atcacttctt gatcgtaaat aacggtataa ggaatttcgg cataggtaag cactaatgtc
     3901 accgcatcat cccatggttg ttttgttttg ggagagtaca cagccatttt aggtgctttc
     3961 tccagtttca tcacatccat attcacttcc ggatcagcaa tgtcggcaac gatcttgtta
     4021 tatgatgcat ccggtatcac ttcataactg acacctcgta tgagacattc cttctcatat
     4081 acatccagat actgaaaaca aaagcttcca ccacggtaat tcaacatcca atcaactttc
     4141 cctccattct ccaacagcca gtaagcaatg ccatagcatt taagatgatc tttctgggtg
     4201 ttgtccatcg gcagcaggat ataagaagcc ttggctgcca aaccggaaat caaaaacagc
     4261 gagatgatga ggtatctttt cataacaagt aacaaattta acgcaaaaat tgagccatgt
     4321 agggtcagtg ggtgagtggg tcagtgggtg agtggtgagt agtgaatggt gagcctgcct
     4381 cgccggtcag gcgggtggtg agcctgcatc gccggtcagg cgggtgggtg agtgaggggc
     4441 actctctgcg gctctgcgac tctgcgtgaa accaactctt ttacttagat aaattctaaa
     4501 taagcatact ccacagcagt tgtgaacaaa cactgaggca atgcattttt aattcagcat
     4561 tcctaatata catttgcaat cgagtaaaaa tctacaaatg aattggttta gcacgttctt
     4621 gacttcatca atcgggcgca aggtggtgat ggctgttacc ggccttttcc tcattagctt
     4681 tttgattgtc cattgtacca tcaatgcaat gatttttttc aatgatggcg gagaatcctt
     4741 caacaaggct gcgcatttca tgggcacgaa tgtgattatt cgcaccatgg agattgtgtt
     4801 gtttttgggg atcattctcc atatcgttca aggcttcatg ctatcggcaa agaatagagc
     4861 tgcgcgtcca accggatatg ctgttactaa tcccggtgct aatagcaaat ggtacagccg
     4921 tagcatgacc cttctcggca ctctgatctt gttgtttttg gtgatgcata tttcacattt
     4981 ctggacgcat agtcgcctcg gaggtattgg tggtatcagt ccgctcgacg aaacgatgat
     5041 cggcgataaa gaggtactca atttgtatgc cttgatgatc agcacttttc aaagtcctgt
     5101 ggtgcttatc gtgtatatac tcggtgtaat ttcattgtgt tggcatttgg tacacggatt
     5161 tcaaagcgca tttcagactt tgggagtaaa tcacaaaaaa tatacgcctg caattaaagg
     5221 tatcggcata gtattcagca ttctgatttg cgctttattc gctgcaatgc ctttatctat
     5281 ttatatgggc tggatagcat aattgtataa actaaacatc aataacccaa accacgaatt
     5341 tatccttttt cctttctcat ttttcatctc tcattttaca ttatgttaga ttctaagata
     5401 cctgcaggcc cattatccga aaaatggacc acctacaaaa acaccgtgaa gctggtgaac
     5461 ccttccaaca aacggaagtt ggagatcatc atagtaggaa ccggtttggc cggggcttct
     5521 gcggctgcta cacttggtga actgggatat aaagtgaagg tgttctgctt ccaggattct
     5581 ccacgtcgcg ctcacagtat tgccgcacaa ggaggtatca atgcagcaaa gaattataaa
     5641 aatgacggcg attctactta tcgcttgttt tacgatacca ttaaaggtgg cgatttccgc
     5701 gctcgcgaag gaaatgttta tcgcttagca gaagtaagtg ccaatatcat tgatcaatgc
     5761 gtggctcagg gtgtgccttt tgcccgtgaa tatggcggac tcttagacaa tcgctcattt
     5821 ggtggtgcgc aggtatcgag aactttctat gcacgcggtc aaaccggaca gcagttgctg
     5881 ataggcgcat accagggatt ggagcgtcag gttggactcg gcaatgtgca agaattttct
     5941 cgccacgaaa tgctcgacat cgtaaccatt gatggtaaag cgcgtggtat cattgctcgt
     6001 aacctcgtta caggtgaact cgaacgccat ttcggacacg cagtattgct ctgtaccggt
     6061 ggatatggca acgtattcta tttatcgacc aacgccatgg gatcgaatgt taccgcggca
     6121 tggaaggcat ataagcgcgg tgcattcttc ggaaaccctt gttatacgca gatccacccg
     6181 acatgtatcc ctgtttcagg tgattatcag tcgaaactca ctctcatgag cgagagcttg
     6241 cgcaatgatg gacgtgtgtg ggtaccgaag agtcgcgagg atgcgaaagc gattcgcgaa
     6301 ggccgcaaaa agcctacaga tattgcagaa gaagaccgcg attattatct cgaacgcaaa
     6361 tatccatcat acggaaacct cgctccgcgc gatatttcaa gtcgcgcagc aaaagaagcc
     6421 tgcgacgatc agcgtggagt aggagagagc ggattggcag tatatctcga tttcagcgca
     6481 gccatcaatc gtctgggtaa acatgttatt gaagagcgtt atggaaactt gtttgatatg
     6541 tatgcgcata tcacggcaga aaatccatat gaagtgccga tgcgcattta tccggctgtg
     6601 cattacacga tgggcggact ttgggtagat tatgaattga tgacaaccgt tcccggattg
     6661 tatgcgcttg gtgaatgcaa tttcagtgat catggtgcta accgcttagg agcttctgct
     6721 ttgatgcagg gacttgccga cggatatttt gttattcctt atacaatagg ttcatacctc
     6781 gccggagaaa ttttcaccaa agcaattcca actgatcatc ctgcatttga agaaacagaa
     6841 aaacatgttc gtgaaagtct cgataaatta ctgagcattc aaggcacaaa atctgtggat
     6901 cattaccata aagaattagg taagatcatg tgggattatt gcggaatggc tcgcacggcg
     6961 gaaggcttga agcaggcacg tgaaatggtg aagaaattgc gtgccgaatt ctggcaaaat
     7021 gtgcgcgtaa caggcaaggc taacgagttc aatcaggatc tcgaaaaggc catgcgcgtt
     7081 gccgatttca ttgaattagg agaacttatg atcgttgatg catatgagcg caacgagagc
     7141 tgcggcggtc acttccgcac agaattccag gaagaaggcg aagcaaaacg caacgacgat
     7201 gaatatgcct atgttgcagc gtgggaattc cgaggtgttg ataatgatcc tgcaatgcac
     7261 aaagaggaac ttaaatacga aacagtaaaa ctcgctacac gatcatataa ataacgcatg
     7321 attgttgttg cgaaaattgc aaatagtact attgaaataa acgttgaata aataaatatc
     7381 atgaacgtta aactcaggat ctggagacag cgcaatgcgc aagaacaagg caagtttgtc
     7441 gactatgccg ttaatgacgc aaatccgaac atgtcatttc tcgagatgat cgacgtgtta
     7501 aatgaaagcc tcgtcaacaa aggcgaagag cccgttgcat ttgatcatga ttgtcgcgaa
     7561 ggtatttgcg gcatgtgcag tatgtatatc aacgggcgtg ctcatggtcc acagaaactc
     7621 agcactacct gtcaattgta tatgcgcaat ttctccgatg gtgaaactat taccatcgag
     7681 cctttccgtg caaaaggatt tcctgtaatc cgcgacctcg tagtagatcg ttcagcattc
     7741 gaccgcatta tcgcagcagg tggatttatc agtgcatcaa caggtcaggc tcaggatgcg
     7801 aatgccattc ccataagcaa gaccaatgct gacttagcca tggacgctgc ggcatgtatc
     7861 ggatgtggtg catgtgtggc ggcatgtaaa aatgcttctg ctatgttatt tgtttcagca
     7921 aaagtgtcgc accttgcact cttgccacaa ggcgatcctg agcgcaggaa acgtgcattg
     7981 gcaatggttg cgcaaatgga tgctgaagga tttggctctt gtaccaatac ctacgcctgc
     8041 gaagctgaat gccctaaagg aatcagcgtt gccaatatcg cccgccttaa tcgcgaatac
     8101 ctgaatgcag cgattgtgag cgaggaaatg ccgaagtaat tagccaatta gccgattggt
     8161 ttttttgaag gcaatgggta actctgcttc tctgcgcctc tgcgcctctg cgtgaaaccc
     8221 tcaacggtat tctctctatc taaacgttaa aatacttgcg aagctgatca gttgcatata
     8281 tatttgcagc cgattggctt catattagct ttagaataat gagaagagac atctttcagg
     8341 caattgcaga ccctaccaga cgggcaatca tcgctttggt tgcgctgcat gcaatgacac
     8401 ccaatgccat tgctgagaat tttgacatca ccagacagtc ggtatctaaa catttgcgta
     8461 ttctgactga atgcgagcta ctcaagcaaa ctcccaacgg cagggagatt tactactccc
     8521 tggaagtatc taagatgaaa gaagttgacc tatggttgga acaattcaga aagttgtggg
     8581 aaggtcaatt tgctcaacta gatcaattac tcgaacaaat gaaaaaacaa taacatggct
     8641 gacaatttac cattagaact taccatcgac aaggcctcga aaacggtaac catcaccaga
     8701 gcatttaacg catcgcagga tatggtttgg gatgccttta ccagatccga atttttagat
     8761 cagtggaatg caccaaaacc attcacagca agaacaaaat acatggactt ctcagttggc
     8821 ggaaagcgtt tctatgccat ggtgagtccc gatggacaag aacgctggat attacaaaca
     8881 tatcttgtca tcaaccccaa aacgcatttc aaatatttca acgtatttgc cgatgcgaat
     8941 gaaaatcctg atcaatttgg atccgagtgg gatatttcat tttcagagca agccggttta
     9001 acaacagttc aaatctctat tttcaatgag tcgctcgagc gcttggaaag aatgcttgaa
     9061 ctcggtttca aagaaggata tgctgctagt atggcgaatt tggaggcgtt gttggaaaac
     9121 atgcaataat ttcaatgatc tgatttgtat tggtagtttt gttaccacac atatttggta
     9181 agatcatgaa acagaaaata tatcaggtag atgcatttgc agaaaagctt ttttccggaa
     9241 atcctgcagc agtttgtccg ctggatcaat ggccggaaga ttcgcttatg cagcagatcg
     9301 caatggagaa taatctggct gaaactgcct tttatgtaaa taatggcgat tactatgctt
     9361 tgcgctggtt cacgccgact gttgaagtgg atctatgcgg acatgcaaca ttggctgctg
     9421 cttttgtttt gtggaattat gaaggacatt catcagatga gattaaattc atttcccaaa
     9481 gaagtggtga actcaaggta aagagatcag gtgcaatgat gacattgaat tttccaacag
     9541 atacactttc agaaattggg gtttcggatg atatcaaagc ctgtttcaat atcagtccct
     9601 tgtctgcatt taaaggtaaa accgattata tgttgatctt caacaatgaa agcgagatca
     9661 cgaatattat tccacatctt gatcagataa acaagctcat cgctagaggt gtgatcatca
     9721 cagcacccgg tgatgtttgc gattttgttt ccagattctt tgctccgcag gcaggtgttc
     9781 ctgaagatcc ggttacaggc tcagcgcata catcattaac accttattgg tcgaagcaat
     9841 tagggaaaac ggaattgtcg gcgatacaac tatcagcgcg caaaggatat ttaacttgta
     9901 agtatctcgg cgacagggta gagatcagtg gaaaggcggt attatattta gttggagaaa
     9961 tcaatgtcaa ataataatag atcaagtatg tcgatagaaa cacaaattca gcaatatatc
    10021 agcagtcagc cggaagcaaa acggaatgat ctgcttgcat tacacaaatc gatactgtac
    10081 ataaaaccaa aagctaagtt gtggttcctc gatggcaaga atgaagcagg caaagtagtt
    10141 tccaatccga atattgggta tggactcctt caactcaaat atgcagatgg ttctgcaaaa
    10201 gatttttatc aggtgggact gagtgcgaat accagtggaa tctcagtgta tatcatgggg
    10261 atggccgata aaaaatatct ggcagatacc tatgctcaag caattggaaa ggcgactatc
    10321 accggatatt gcatcaagtt taaagcactg aaagatatca atatggatac tttacagcac
    10381 gctattaaag atggatttgc tgcttgatcc tttttcgaac ctgattacat aaaaaatgaa
    10441 attatttctg atcagtttgg tggtcatgtg tttttccgcc tgaaaacatt tgacagagga
    10501 gacttcaacc gaggtgcagc aaacagatta ccagggtatt tcagttgaag aattattgaa
    10561 aaaagcgatg cttcaggata ctatgaaatt tgacagatgg gaaccgttta tctcttttgt
    10621 atcatatcgt tcgcaggaga ataaactgta tgctttcgca attacacgtc atacagattc
    10681 aaccatgctg atctcgcgtt acgcattaca agattctacg tggaagttga tatcatctct
    10741 aagtggctta agggggccgg gtgtttttta tcatctcaat gttgctgact acgattttga
    10801 tggtgttcgt gatgttttca tcactagtgt aacttctaat ggtattgcat tatcgcaagg
    10861 aaacttagtg ataacagatg atattgataa catacttgta taccatccgg aagctgatac
    10921 attatattca ccaatacctg atccaaatag gaaattagtt atgtcgcaaa aggttataaa
    10981 ctgtgcagaa accggattcc gcaacgtttg cgatatcacc aataaatggg tcgatggccg
    11041 attggtaact actgaacaaa attgcccttg tgagacagaa tgataagcga ggcttataaa
    11101 taagactatc gccagttcaa aaatacgatt gatatttttc atttgcttga tctccggaat
    11161 aggcataatt agttgatatg tatatatttt aacaatcaga aaatcaatta gttatacttg
    11221 caaagtcact gcataggcat caatttttga acgggtctga tcagaaaagc tttgcatgat
    11281 ttttgataat aaaaattgtg gggttaacgg ataaatatta ccggaacatt gcaataatcc
    11341 acctccattc gtcgtaaatt agcaactgat gttgaagaaa cgcgtttgta aggctttgat
    11401 tttcatgctg ctggtggtgg tttcggcccc taaagcctgg gccacccata acagggcggg
    11461 ggagattact tatacccatg ttgaagatct tacttacgaa ttcaccatca caacctatac
    11521 agatccggca tctttggccg atcgcgacag gcttggaatc gattggggcg atggcacggt
    11581 tgataccatc atgcgcaaca gtattgagtt gctgacacct gatatccaga aaaaccaata
    11641 catcagtacg catactttcg ccggtcccgg tatcttcatc atcagtatgg tggaccccaa
    11701 cagggtggaa agtatcctta acattgatgg ttctgtaaat gtaccttttt gcctggttga
    11761 cacgtttatc attttcgacc ccctgacttt gggatacaac aattcgcctc ttttgctgta
    11821 cccacctatt gattatgcga atattggtga gtttttcatc cacaatcccg gtgcgtatga
    11881 tatcgacggc gactccctgg cgtataagct tgtggtgcca ttccaatatc cgggtattgt
    11941 agttcctggt tatctcgatc ctgatgatcg caccttttgc actgatacgg atgtttttca
    12001 aatcaatacg ttcaatggag atgtcacctg ggaggatccg tcgtgtaccg aagggatata
    12061 taatatcgcc attctcatta cagaatatcg caatggtatc aagatgggca cgatcctgcg
    12121 cgatatgcag atttttgtaa ataagacaag caatcatgcg cctgaaattg cagagcttaa
    12181 cgacacctgt gtttatgcag gagaggaatt aaatattctg gttacagcaa ctgatcctga
    12241 tgttttgcaa acaattacct tgactgctac aggtggacca ttatatattg atgaaagccc
    12301 tgcagaattt ttcagcacac caaatgaagg ttcggtgaac ggaacattta catggcaaac
    12361 tatctgcaac catgtaagaa aggaagtgta tgaagttgta tttaaagcag aagatgattt
    12421 tcaaatcggg tttgaagaaa ttccgcttgt cgacctggaa acatggttga taacagtagt
    12481 agctccacca cctgttattc ttgatgcagt gccgacagca aatgagataa ctgtgacctg
    12541 gcaggatcca tatatatgtt caggtgccga taattttctt ggatttagtg tttggcggaa
    12601 aattggttgt gattcaacaa tgtttgataa atgtcaacgc ggactttcag ggatgggata
    12661 cgactcttta gcatttgttg aagatgccta ttcctatgtt gatttggctg ttgactatgg
    12721 acaggaatat gcttatcgca tagttgccga gttcggtgaa cgcagtgata tcgcacccga
    12781 ttttgtttac aatcaagtac ccggagcgcc atctgatgct gtatgtgccg aattgaaacg
    12841 cgatattccg attattaatc atgccagtgt tcgcaccaca tcaacaacta atggaagtat
    12901 gtatgtgggt tggtataatc cgatttctcc tgatttagac actaccattt ttacaggacc
    12961 gtacaaatat gaattggtgc gttatgaagg ctttacacca acaggtaccg agacattagt
    13021 gacaacgatt acagcaggtg agttttttga aataaccgat acttcgtata tcgatactat
    13081 gttgaatacc gcagcaactg cttattgtta tcgattgaaa ttttatttca ccgatctcgg
    13141 aaattttgaa ttattaggtg ttactgatcc tgcctcatcg gtgtatttaa cagcatcacc
    13201 cggcgataat aagctgaatc tttcttggaa ttttaatgtg ccttggttga atttttatta
    13261 tgatgtttac aaggaaacca ctgcgggctc cggtgatttc gaattgttaa caagtactac
    13321 agagacaagt tatatcgatg atagtttggc gaatggttct gagtattgct actatgtaaa
    13381 agcctatggc aaatatacca ttgagtcgct tccggataca ttaattaatt tatcgcaaat
    13441 acaatgcggc gttccgatag acaatgagcc tccttgtcca ccgatattga cagtctataa
    13501 tatttgtgaa gaggatgggt cgatagttgt tgatgaatcg gatttgaaaa atgatttgac
    13561 ctggacaaat ccaaatcatt catgtgccga cgatgtggtg aaatatctga tttattacgc
    13621 ttcgcaacaa ggtgatcctt tgacattatt agatcagaat gccgatgcat ttgatactac
    13681 gtatacacat gatggattgc tttcacttgc cggttgttat gctgtagttg ctgttgactc
    13741 gtttgataat aatagtgctt ttagtaatat tgtttgcgtt gataattgca gtgattataa
    13801 tttgcctaac accttcactc cgaatggtga tgggtttaat gatatcttca caccaattct
    13861 tccatacaga ttcattgatc atgtggatat gaaaatcttc gatcgctggg ggaatgtagt
    13921 atttactaca aatgacccta tgctgaattg ggatggtatc gatacgaata caggaaagaa
    13981 tgcttctgaa ggtgtgtatt tctatacatg tacagtatat gaaatcactg tcgatggcgt
    14041 cagacctttc ccgcagccta aggaaggtta tattcacctg tatcgcgaga gcaaggtgga
    14101 ataacagtta gggtgaagtg ttacttccct tgttatgaat attgtgtttc aaaaagtgca
    14161 attcctatct ttgtataatg ttcaccggga ttattgaatc attgggaagt gtttcgggat
    14221 tgcaaaaaga tgcaggaaat ttgcatatta ctgtccaatc cgaaatcagt cgtgatttga
    14281 aagttgatca aagtgtaagt cataatggtg tgtgtttgac ggttgttcat caagccggcg
    14341 atacacatac agttactgca atcgatgaaa cattgattcg ctccaacctg ggtaatttaa
    14401 aaatcggaga tacggttaat ctcgagcgct gtttaaaaat agacggtcgc ctggatggcc
    14461 atatcgtaca aggtcatgtt gataccaccg cgcaattaat tcaggttgac gaacaaggag
    14521 gaagctggtt gctcacattt cgattcgatg cgcaatttga taaatacatc gttcaaaaag
    14581 gaagtatatc cattaatgga atcagcctga cagtttttaa tgtttctgag ggaacgtttc
    14641 aggtggcaat aattccttat acatgggaac ataccaattt gagccaaata tccgtaagtg
    14701 attcagtgaa tatcgaattt gacattatcg gtaaatatgt cgaaaaaatg atggcccgtt
    14761 gattgttttc cggttttgta ttataatctc acggaaaatt tatttccaac ttaaatcgtc
    14821 tttcctttca ttgcctggct gatagccata tccatgagtc gctctgcaaa tggtcgcgta
    14881 aactcattta gaatttcaat ttgctggtgg atgacatcct tgtcatcgga attaagtgta
    14941 ttacgtagat tgtcgattaa tgatttggtg cctgctattt cctgttcatt taaatactga
    15001 ttgtttttct caataaatcg ctcacacgtg tctatcatat tctgtgcttc aactctggct
    15061 tcaacaattg ctctttcagc catatctgtt ttggcgtttt taatagagtc gagcagcatg
    15121 gcggccattt cttcttctgt taatccgtat tgaggcgtaa cttcaatttt ttgttcaaca
    15181 ccactacgca attcttttgc acgcacttga agaatgccgt ccgcatttat ttgaaaaaca
    15241 atttcgattt tcggcaatcc ggccggcata gcaggaatat tggataaaat aaattcagcc
    15301 aattttctat tgtcgcgcac tagatctcgt tccccttgga atacagatat tttaagattg
    15361 gcctgaccgt cgagactagt ggtatattgc cttcctgcag cggttggaat ttttgtattg
    15421 cgtggtatga tgacgtccat gagtcccccc atcgtttcaa tgcctaagct gagtggagta
    15481 atatccagca gcaacatgtc tttttggttg cctgcaagaa tatcggcctg aacagcggcg
    15541 ccgagggcaa caacctcgtc agggtcaact tcatcgtgaa caggtttatt gaaaaatgcc
    15601 gaaacgcttt tcttgatgaa aggtgtgcgc gtgcttccac caaccataac aacagtgtca
    15661 atatcgttca cattgagatt tgcatcaaac aatgcgcgtt cgcaacactg aattgttttt
    15721 tgtagcagtg gtgtaattaa tgtttcaagt tctgcaatgg ttagatcgca ttgtatttta
    15781 tcaacgttgc cggtaaattc accatgagaa ccaaggtgtt tttttgcagt ttctgcggtg
    15841 agtcgcaaat cctgacgaac ggttgctgat aataagttgc catcaacatg ttgagtttgc
    15901 agccaatgat ccatgatggc acgatcaatg tcatcgcccc ctaaaaaagt atcgccattt
    15961 gtgcttaata cttcaaaaaa atcgcctgag ttttccgagc ttgtaatgcg tagaatggag
    16021 atatcaaagg tgccgcctcc aagatcatag acggctatgg tatgtgacga tttatcttga
    16081 tttcccaagc catatgcgag acttgcagcg gtaggttcat tgataatacg caatacatcc
    16141 agacccgcca attttccggc atctcgagta gcttgtcgct gagcgtcatt gaaatatgcg
    16201 ggaacggtga tgacagcttt tgttattgca cattgaagta aacgctcggc acgtaatttt
    16261 aattctttga gtatcagagc agaaagttcg ataggggaat aatatttttc gcgaacctta
    16321 attttcacca aacgttcggc gtcatcatca attattttat aaccgaaagt atgttgatat
    16381 gcggctatgt ccttatatga ttttcccatc agacgtttga cagaaaaaat ggtatgctca
    16441 ggatccgtga taagatgttc gcgggcgctt ctaccaacag taatagtgcc atcatcagca
    16501 aaatgaatga tagagggtac tatggcgaat ttatcggcat cgcgtaatgc agaaggggtg
    16561 cctgtttctt tatctattat ggcgataagt gagttggtgg tgcctaaatc tataccgaca
    16621 ataatttctt ccctttttaa tgttccttct ttgatattga tgctgatctg tgccatagtc
    16681 ttataacaat gcccttgaaa atgagttcgc cgcaaagata agaacatggg aggaggagag
    16741 aggagaaagg aggaaggagg aaggagaaag gaggaaggag gaagggagga agggaggaaa
    16801 gtggatggag gaggtgtgtt tgttttttta cttctggttt gagggtttga gggttaagct
    16861 tatcggcttt tctcatttac tttgtacttg gatgtataag accttattaa aaccgctgtt
    16921 ttttcgattt cagcctgaaa cggctcatca tttggcatta gggctctaca aggctgcttt
    16981 gtggattccg ggatatggat ggtggcacga taagcattat gtgattgagc ggccggaatt
    17041 agagaaagaa gttttcggaa tcaggtttaa gaattctatt gggctggcag cgggatttga
    17101 taagaatgcg gcctttattc ctctgatggc gaggttaggg tttggacatg ttgaagtagg
    17161 aacggttacg ccgaaggcac aaccgggtaa cgataaacca cggttgttta ggcttcctaa
    17221 agatgaagcg cttattaatc gcatgggatt taataatgaa ggtgttgaaa tgatggtgag
    17281 gaatcttgga aaatcatcgg tagcgcatgc cagagagaaa tatcgtgtgg tgattggcgg
    17341 taatattggc aagaacaaag tgacagaaaa tgaggatgca tggaaggatt atgtcatttg
    17401 cttcaacgcg ctattcgacc atgtagatta ttttgtgatc aatgtatcaa gtccgaacac
    17461 acccggtctg cgggccttac aagaaaaaga accgcttaag cgtataatct ctagtctgca
    17521 agaaataaat cagggaaaga aaaggccaaa gccattgttg ttgaagatcg cgccggacct
    17581 gaatgatgcg cagttaaatg atattgttga gatcacacga gaaacggggt tgagcggtat
    17641 catagcgact aatacaacct tggatcgcag tatgctgaaa acaccccagg ctgctgtgga
    17701 gtctataggt gccggagggt tgagtggaaa accggtattg tcaaggtcaa atgacgtact
    17761 ttggtttcta agaagggctt taggaactga tgttccattg ataggggtag gaggggttac
    17821 ggataaagcg tcgatgcaat caaaattcga caggggggcg gatctagttc agatttatac
    17881 ggggatgatt tatgaagggc aggggatggt gaggggggtg ttggtgtgag ggggatttga
    17941 gacctaacac atctaaaacc tgccagattt taaacctaac agatcttaaa cctaacaggt
    18001 ctaagacctg ttaggtgtgt ttttttgaga tgccctattt ccaacccata aacccgaata
    18061 accgcttcta atcgcttact cgcgttacta aaagcatgcc tctttcccga accggaacgt
    18121 cacctcaccg cgggcggcgc atctgcaaat ttcctatact tgcctgccaa tttcccaaag
    18181 aacactacta ttggaaatat cacattcttt acaaatcccg ggattgcagc tacatcaaac
    18241 tccgaacggt atttccgaat gaggtaggca gcctcaaaac cccccggctc cccacctccc
    18301 tcattctgca accgatatat attctccaaa taataaatga tgttgttcgg tggtgatatc
    18361 cacccttcgc atttcagcac atcatcaccc gcattccaaa aacgatgcat c
//
LOCUS       DAOQHY010000051        18374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1398_length_18374_cov_6.699383, whole genome shotgun sequence.
ACCESSION   DAOQHY010000051 DAOQHY010000000
VERSION     DAOQHY010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 18374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18374
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1398_length_18374_cov_6.699383"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="PLL28_05940"
     CDS             complement(<1..864)
                     /locus_tag="PLL28_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68898.1"
                     /translation="MRKYILFIVSLLLIPATYAQTNISGVINIYTVVDSVNNCSNQVY
                     TTSAAGFSVGDRVLLIQMKGAQVNLTNTASFGNLTAYNNAGNYELATISAISGSVISF
                     QNGILRNYTALSAIQLVRVPVYGDVNIVGTLSAADWNGSTGGILAFKADMVTFSADID
                     VSGKGFRGGNAGNYPSSCPTGTGTSLYYSDTLSGKGGAKGEGIVILTSGYLACRGKAF
                     NGGGGGNDHNAGGGGGANGGGGGVAGENDEAFFSCPGSPGFASLAPDQTMAADKLFLG
                     GGGGAGHGNNNN"
     gene            929..1966
                     /locus_tag="PLL28_05945"
     CDS             929..1966
                     /locus_tag="PLL28_05945"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNF68899.1"
                     /translation="MANKLGINVKMFIFFMLLQQGAFSQAIPADSMFSLIKQYNAYGA
                     GADWASIDKQFHQSLISAQTSDDTIQSLIHVFATLDDVHTTFQINGKTYGFYHGVTAE
                     ESAQLQPLMQRQREESGKIFTTKLPGDIAYIRIPTLNIWGDDIHAYTKTIQDSLLNIL
                     DKNTIGCIVDLRLNGGGNMYPMLAGLYPLLGNNTVMHITNPDSTVATTWFLKKGNLYG
                     ENIYGDKVPVTNVDYTSKENYDNLKVMVLIGPVTMSSGQATAVAFYKRKNTLFIGEPS
                     ATGYATAKNYYPLPNNCFMNMSSGFISDREGELYPQVVNPQLWVIHGDNFDKLSEDLK
                     VQMSTWRLTDK"
     gene            1998..3185
                     /gene="kbl"
                     /locus_tag="PLL28_05950"
     CDS             1998..3185
                     /gene="kbl"
                     /locus_tag="PLL28_05950"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HNF68900.1"
                     /translation="MYTIQDRLQHEIQDIKESGLYKNERIITSPQGADITLADGSKVV
                     NFCANNYLGLSSHPKVIEAAKKAVDSHGYGMSSVRFICGTQDIHKTLERKISEFLGTE
                     DTILYAAAFDANGGVFEPLFGEEDAIISDALNHASIIDGVRLCKAVRYRYNHNDMADL
                     EVQLQAAQAQRSRIIVTDGAFSMDGTIAALDQIVALAEKYNAMIMIDECHASGFLGKT
                     GRGTHEYRGVMGKIDIITGTLGKALGGASGGFTSGRKEVIELLRQRSRPYLFSNTLAP
                     SITGASIAVLDLLSETTELRDKLENNTMYFREKMTAAGFDIKPGVHPIVPIMLYDAVL
                     SQQMAAKLLEEGIYVIGFYYPVVPKGQARIRVQISAAHDQHHLDKAISAFVKVGKELG
                     VIK"
     gene            complement(3202..4296)
                     /locus_tag="PLL28_05955"
     CDS             complement(3202..4296)
                     /locus_tag="PLL28_05955"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNF68901.1"
                     /translation="MASMIEQPGNEQEKQINKQFSYTRIAAVIALSLGISAYLIYTSF
                     DYKAISHIQWAQDAFGWLAFGALMMVARHIGYMYRLRLITDNKLSWYQSFNVISLWEF
                     SSAITPSTVGGAAIAIYFMKKEKLSLGKSAATSMLTIFLDQVFLAAGGLILFVLLGGN
                     AMFATDAQCRSQSELPMMSAFHNMQTIYFLGYGLFAIIISLLAYGLFINAHAFKSLLS
                     SLFSLPILKKWRNDALQTGDDMILTSHELKHKSKGFWWKLFISTAITWIAFFMIPICI
                     VHAFFDPTLADLPVIFSRMFAIWMLMLLPISPGGAGIAELTFSAMMCDFTDPALVATL
                     ALLWRLLSFYPYLLLGVVILPRWVNRKYNK"
     gene            4487..4954
                     /locus_tag="PLL28_05960"
     CDS             4487..4954
                     /locus_tag="PLL28_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68902.1"
                     /translation="MSSGLVMYLRTTHARREVIVQYSRTENQPETTILKLSGNEFTAL
                     EKERISEKIFEIQWKGERYDIIDLQYQQNGDVYLTAHHDKKEAKAIKDFADAHQDSAN
                     AGNLIFFSFCDGIIHTDIIHFIPSEIFLHSEYNVILHRHSISPTSPPPDFLAA"
     gene            4983..7022
                     /locus_tag="PLL28_05965"
     CDS             4983..7022
                     /locus_tag="PLL28_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF68903.1"
                     /translation="MTLFRNGLLVLATMYLYASVCFAQTDTVTLDRIVVTSFKDTRLR
                     ESSLTITPITLSDIAQRGAFNISDALAKTPGLSQLGTGNAISKPVIRGLYGNRVLILL
                     SGMRFDNQQWQDEHGLGLSEVGIGSVEVIKGPFSLLHGSDAVGGVINIIEEDKSDPFH
                     IDQDVSVKFNTNTLGEMVQYGYKRNDGAHWIGIRLAAESSPDYSDGNGTRVLNSRYDG
                     YYLKGNYGFKKGNWISDNHYMGSFNRYGFIFSDIRDFFETPDARFSRSFPGPHHIVAL
                     NIGTSENTIILPKSVLKVNVGIQSNARLEDEGSGSISLFMHLVTGQYATRWTKSLNQR
                     NTLIIAHNASIENNTNYGKKVIVPDAWMAEGNLAAYVSHSSGKLIFETGIGGGDRYIK
                     TLYTAGVNSPEKDIDPFSRHWLFANVLAGLTFNPNNNWNIKSHVATGVRAPNLAELSS
                     NGLHEGVFTYEIGDPNLHTEKNLSLAADINRTGKYITASISGYYNFFADFIYLSPTDE
                     EWFGFPIYRYLQQSAQVYGAEAFISFRIPAFPYLTYSITTADVEGKLKDGAYLPFIPA
                     LKVTPEIKFKGLHDEHNWYAFVNTDIYSPQHQTATDEIATPGYTLLNAGVGGEWHIGK
                     YPLEINLVGNNLLNEAYYDHLSRFKSLGYLNMGTNIQVHAIMHFQSTFKYHNKTN"
     gene            7028..7459
                     /locus_tag="PLL28_05970"
     CDS             7028..7459
                     /locus_tag="PLL28_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68904.1"
                     /translation="MKHFMKLNTLIIAVAAITFALASCNKDEMEPPIISFKTGAGYTS
                     DDITIAAGSSVLIGIEASKAEAEGEEEDVLSHFNISLSVNGGASTSVYDADMTAAQEE
                     EYSYDYNAAASTTVGEVDTYTFTITNRDGLTGQVSLTVTSN"
     gene            complement(7531..8280)
                     /locus_tag="PLL28_05975"
     CDS             complement(7531..8280)
                     /locus_tag="PLL28_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNF68905.1"
                     /translation="MQNQQTFEIKLPQFEGPFDLLLFFIERDELDIHDIPISRITKDF
                     LDYLHSMTQMNIEIASEFILVAATLMRIKAKMLLPRKELDEFGNEIDPRKELVDRLLE
                     YKRYKEVLGHLSELEDDRQLRINRGNIPIEHDILAKKHSNEMELSSVNLFVLLKVFQK
                     VMDRYNNQPKVQEHVIVQYNYSIEEESFGVLSYVRGKKNVPFADVFNSCKDKYQAVFR
                     FLSILDLIATQKVTLLVGEGINNFWLTSDQD"
     gene            complement(8375..10576)
                     /gene="recQ"
                     /locus_tag="PLL28_05980"
     CDS             complement(8375..10576)
                     /gene="recQ"
                     /locus_tag="PLL28_05980"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HNF68906.1"
                     /translation="MDVSHLTLKDALQEYFGFDSFKGNQEEIITSLLNGQDTFVIMPT
                     GGGKSLCYQLPAMMLEGTAIIVSPLIALMKNQVDLVRSYSSNNDIAHFLNSSLNRQQI
                     KKVKADIVAGRTKMLYVAPETLRKEENIEFFRGIRVSFVAVDEAHCISEWGHDFRPEY
                     RQIHSMIEAINDDIPIIALTATATPKVQSDIKKNLDLEEPNVFISSFNRPNLYYEIRP
                     KRSREQAIKQIVKYIKEQGRKSAIVYCLNRKSTEDIANILNINGIKAGAYHAGLDAAL
                     RSEVQDKFLMEGYQVIVATIAFGMGIDKPDVRYVIHFDIPKSIENYYQETGRAGRDGL
                     EGKCIAFYSYKDIEKLEKFMRDKPLSEREMGAQLLAETAAYAETSVCRRKFLLFYFGE
                     EYKQENCGSCDNCLKPKEKIEGKEFVVNALKTIDVINENHHIKYVVDLLTGKRTTENQ
                     NYRHDKIELFGVGKEKDDQFWNSIIRQCLLSELVYKDIEQYGLIKLTDKGRNFIKKPH
                     SIMVSLNHDYDQEVADVDMLESNAPKSVLDPVMMDILVKLRDKVAKDAGLPPYVIFSE
                     NSLEEMATMYPTVMDEMVKISGVSRGKAEKYGRKFLEAIKKYVEENDVEKPSEFVMKQ
                     VVNKSAHKVKIIQFIDKKMPLPDIAKSIGLNFPDFIDEIETIVSSGTKLSLNYYLDDI
                     IESDLQEEVHDYFIEMSSLDLVSAYNDLKDEGLSFEEVRLLHIKFMSEMAN"
     gene            10736..11815
                     /locus_tag="PLL28_05985"
     CDS             10736..11815
                     /locus_tag="PLL28_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HNF68907.1"
                     /translation="MSNVYVVIMAGGIGSRFWPESRVKKPKQFLDILNTGETLIQTTY
                     TRFLKIADKDNIFIVTNEQYISLVNEQIPGLNPLNILAEPSRKNTAPCIAYACHVIAQ
                     RDPEATVVVAPSDHLMLNPDKFLLTVLEALEYADSHDVLLTLGIKPTRPDTGYGYIQY
                     EEASVAGNIHEVKTFTEKPDQELARTFIKSGDFLWNSGIFIWSVKSIMSAFDLHQQDI
                     HQAFWAARKAFGTKDEKWMVEKAYSICPNISIDYAIMEKADNVYVIPASFGWSDLGTW
                     ASLWDNYDKDYYGNAVKGKKVMIYDATNNMIMSDDDKLVVVQGLEDYIIVDTKDVLII
                     CRKDKEQEIKDITSDLKRKEMDDFL"
     gene            complement(11936..13024)
                     /locus_tag="PLL28_05990"
     CDS             complement(11936..13024)
                     /locus_tag="PLL28_05990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF68908.1"
                     /translation="MATICFFNSIRFWGGGEKLHLELAVGCKALGHHVVIACNKNGIL
                     WQRAQEAGLECVDVRVTKFSFLNTFHINKISNTLASIQANAIIFTASQDAKTGGAAAK
                     KAGINKIVYLRGLAARVKNSYFNRRLFTKTLTHIIANSEETKSLLLQKMQHVKGIPNI
                     PVVYHGINIVETQSRNKHPFIAQHGNGIILGNAGRLTAQKAQKDLIEVAIKLRDLQIN
                     FTLFIAGEGEERTSLENAIKLHQLQNQVFLMGFVDDIPAFMQSIDIFVLSSHWEGFGF
                     VIAEAMSHELPVVAYDISSNPEIIADNETGLLVPCGNTDAFALAIKQFAEDQDLCKKY
                     GIAGRKRVIEKFEFSTQLKQLLAVIEIN"
     gene            13126..14283
                     /locus_tag="PLL28_05995"
     CDS             13126..14283
                     /locus_tag="PLL28_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HNF68909.1"
                     /translation="MADHTVQIKSKLPNAGISIFARMTALANQHGAINLAQGFPDFMS
                     SEKLISLVHAYMQKGMNQYAPMPGLLALRERIAEKIKFIYHNTYDPASEVTITAGATQ
                     AIYTSIAATIRDGDEVIIFEPAYDSYAPAIRVNGGIPVPVELKAPDYKIDWNIVKKLV
                     SQRTRMIMINTPHNPTGTVLDQSDIKQLIRIVHGKDIIVLSDEVYEHIIFDGIRHESV
                     LHYPELRERSMVIFSFGKTYHNTGWKIGYVLAPEYLTKEFRAVHQFEVFSVNTPIQYA
                     LSDFMEDRSEYEKVSNFYAAKRNLFSDMLKGSKFVLKPSAGTYFQLLDYSAISDLGDQ
                     EFADLLTIEHKVAAIPLSPFYRNGSDAKMLRFCFAKQDSTLQKAADILCKI"
     gene            14271..15068
                     /locus_tag="PLL28_06000"
     CDS             14271..15068
                     /locus_tag="PLL28_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNF68910.1"
                     /translation="MQDLKVTLVQVDLAWENKQANLDYISTLLKDVRDTDLIILPEMF
                     STGFSMRSKDLAENMMGNSVRWMRDLASAKNAAVCGSLIIEDDEKFYNRLIWMEPDGT
                     SAHYDKRHLFSLAGEDKHYTGGKHKIFPELKEWKILPLICYDLRFPVWSRQSPPFTEL
                     GANPYHLLVYVANWPERRVYAWEQLLIARAIENQCYVVGVNRIGMDGNDVYHNGCSLV
                     VDPMGQKLYESKGEESVKTLTLSGLQLESIREKLPFLDDRDEFELRR"
     gene            15163..16017
                     /locus_tag="PLL28_06005"
     CDS             15163..16017
                     /locus_tag="PLL28_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNF68911.1"
                     /translation="MITDKIVKPSSGFFMFFVFLLMSIGIGYAMMNVHPAYSLAFIPL
                     FFVLIGFFIINPNDAKVLVLFGKYAGTVTENGFYWANPFYGKHRVSLKARTLDGDKIK
                     VNDKLGNPIIIGAVVVWQVDDAAKAKFEVENYEMYVKLQSDTAVRHLAGSFSYDHFSD
                     NDSEEITLRSGGEHINEMLVKELKDRLQLAGVKVIEARITHLAYAEEIAGAMLQRQQA
                     TAVVAARTKIVEGAVGMVQLALQKLSKEQIVELDEEKKAAMVSNLLVVLCSDKTANPV
                     VNTGTLYH"
     gene            16027..16530
                     /locus_tag="PLL28_06010"
     CDS             16027..16530
                     /locus_tag="PLL28_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68912.1"
                     /translation="MEFQEEQRISVMWMRILFLIIIIGSLAGVLLGGMNSGHEPQISD
                     YLGAVFCVAIVSLVYYMVFYTKLKTSVSMMGFAYEYFPFVWREKTITKEMIKSWQMQT
                     MKSGFQYGGYGIKQSYFPKKTAFMMGSRNAVEFKLHDGKTLIFTTNNPEQLQLALRKY
                     FSQTEIR"
     gene            16530..16718
                     /locus_tag="PLL28_06015"
     CDS             16530..16718
                     /locus_tag="PLL28_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HNF68913.1"
                     /translation="MAKEDNKSAKDRQAFILRVSPELLKAVEKWAADDFRSVNGQIEF
                     LLHEALKKSGRMKKRIEE"
     gene            complement(16722..16895)
                     /locus_tag="PLL28_06020"
     CDS             complement(16722..16895)
                     /locus_tag="PLL28_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68914.1"
                     /translation="MVDKFQQWRFTSYHLAISSEHNTIQSNVLPLFGGKAKCIDFHKA
                     FIQSREAERVFTE"
     gene            16949..17704
                     /locus_tag="PLL28_06025"
     CDS             16949..17704
                     /locus_tag="PLL28_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68915.1"
                     /translation="MGRSGDVVNFSGYIWDVKIAESYQVGPGPNYFSGYYEDVFVDEQ
                     GYLHMRIAEHDGKWFSSEVVSQMNMGYGKYSWVVQGDLENIPENITLGLFTWDNNTFF
                     DQANSEVDIEFSKWGVASEENTMLYSVQPVNFGPTYPERTHLIETEVGDLVGVTTHTF
                     IWTDTLITWNSYYGDTESEDKRIGTWYFNLDNPPRVKNENGNSSAAIVIPAPGETTNA
                     RINFWLLPWISSSPTDGLEREIVIRKFSYEAAE"
     gene            complement(17724..>18374)
                     /locus_tag="PLL28_06030"
     CDS             complement(17724..>18374)
                     /locus_tag="PLL28_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNF68916.1"
                     /translation="YPVLYMLDGQNLFDDQLSYAGEWGIDESMDSICHLGETTAIIVG
                     IDHAGDARLAEYTPWKVARFNESGKGDLFAQFVVEELKPHIDSLYRTLPDRIHTGIGG
                     SSVGGVMSQYLVLQYNDVFGNALVFSPSFWISKQNFQLAEAYTSDKPTAIYYLAGGRE
                     GDDDDMQENAIAMYRLLQKKQINNLTLRIVIEGNGIHTESFWRKGFSAAYLWLFEK"
BASE COUNT         5382 a         3520 c         4004 g         5468 t
ORIGIN      
        1 attattgtta ttgccatgac cggcaccgcc accgcctcca aggaaaagtt tgtcggccgc
       61 cattgtctgg tcaggagcca gggatgcgaa tccggggctt ccggggcaag agaaaaatgc
      121 ctcatcattt tctcctgcca cgccaccacc accgccattg gcaccgcctc cgccgcctgc
      181 attgtggtca tttccgccac ctccgccgtt gaacgctttg cccctgcacg ccaggtatcc
      241 acttgtgaga ataacgattc cttcgccctt ggcgcccccc tttccgctga gggtatcaga
      301 gtagtaaagg ctggttcctg tgcctgtagg gcagctggag gggtaatttc cggcattgcc
      361 acctcgaaat ccctttccgc tgacatcaat atcagccgaa aaggtcacca tatcggcttt
      421 gaaggccaga atgccaccgg tgctgccatt ccaatcggct gcactcaagg ttccaacaat
      481 attgacatca ccgtacaccg gaacgcgaac gagctggatt gctgacagtg cagtatagtt
      541 gcgaagtatg ccattttgaa aggaaataac agacccgctg atggcagaaa tagttgccaa
      601 ctcatagttt ccggcattat tatacgcggt gagattaccg aaagaggctg tattggtgag
      661 gttaacctgc gcacctttca tttgaatcaa caacacacgg tcacccacac tgaaaccggc
      721 agcggaagtt gtgtatacct gattgctgca attattcacg gagtcgacaa cagtgtatat
      781 atttatcact ccgctgatat tcgtttgagc gtatgtggcg ggaatgagta aaagtgaaac
      841 aatgaaaagt atatattttc gcataggttg atgattcaac gataaaatta atcaaatgct
      901 gtttttcgga acaaatctcc gcttttgtat ggctaataag cttggaatta atgtgaagat
      961 gtttattttc ttcatgttgt tgcaacaagg tgcattttct caggcaattc ctgctgatag
     1021 tatgtttagc ttgatcaagc aatacaatgc ctatggagca ggtgccgatt gggcttcgat
     1081 agataaacag tttcatcaaa gtctcatttc ggcacaaacc tcagacgaca cgatacaatc
     1141 gttgatacat gttttcgcta ccttggatga tgtgcatact acttttcaga taaatggtaa
     1201 aacgtatggt ttttatcatg gtgtaactgc tgaggagtct gcacaattac agcccttaat
     1261 gcaacgtcaa cgcgaagaat ccggcaagat ttttacgaca aaattgcccg gtgatattgc
     1321 ctatatccgc attccgactt taaatatttg gggggatgac attcatgcat atacaaaaac
     1381 cattcaggat agtcttttaa atattcttga taaaaataca ataggttgta ttgttgatct
     1441 gcgattaaat ggcggaggaa atatgtatcc gatgttggca ggattatatc cattattagg
     1501 aaataatacg gttatgcata tcaccaatcc ggatagtacc gttgctacaa catggttttt
     1561 gaaaaaagga aatttatatg gtgaaaatat ttatggcgat aaagttcccg taaccaatgt
     1621 tgactacact tctaaagaaa attatgacaa cttaaaagtt atggtattaa ttggtcccgt
     1681 taccatgagc agcggacagg caacagcagt tgcattttat aagaggaaaa ataccttatt
     1741 tataggtgag ccaagtgcca cgggatatgc cactgcaaaa aattattacc ccttgccaaa
     1801 caattgcttc atgaatatgt cgtcggggtt tataagcgat agggaaggag agctgtatcc
     1861 acaagtggta aatccgcagt tgtgggtaat acatggcgac aattttgata agctgtcgga
     1921 agatctgaaa gtacagatgt cgacatggcg attaacggat aaataaattt caattaattt
     1981 cacaccaaat taatcatatg tataccattc aggatcgttt gcaacacgaa attcaggaca
     2041 tcaaagagtc gggtttatat aaaaatgaac gcatcattac ttcacctcaa ggtgccgata
     2101 ttactttggc tgatggaagc aaggttgtga atttttgtgc aaacaattat ttgggattga
     2161 gttcacatcc aaaagtaatt gaggcagcaa agaaggctgt agatagtcat ggatatggaa
     2221 tgagcagtgt gcgttttatc tgcgggacac aggatataca taaaacatta gaacgcaaaa
     2281 tatcagagtt cttaggcacg gaggatacta ttttatatgc cgcagctttt gatgcaaatg
     2341 gtggtgtatt tgaaccattg tttggagaag aggacgccat tatttccgat gctttaaatc
     2401 atgcatctat tattgatggt gtgcgtttgt gcaaagctgt cagatatcga tacaatcata
     2461 atgatatggc tgaccttgaa gtgcaactac aggcagcaca ggcacaacgc agcaggatta
     2521 ttgttacgga tggtgcattt agtatggatg gcactattgc ggcattagat caaattgttg
     2581 cgttggctga aaaatataat gccatgatca tgatagatga atgtcatgca tcagggtttt
     2641 tgggcaagac aggtcgcggc acacatgagt atcgcggagt gatgggtaaa attgatatca
     2701 ttaccggcac gctgggtaaa gcacttggcg gcgcaagcgg tggatttaca tcaggaagaa
     2761 aagaagtgat tgaattattg cgtcaacgct ctcgtccgta tttattttca aatacgttgg
     2821 cgccaagtat tacaggtgct tcaattgctg tgcttgattt gttgagtgag accactgagc
     2881 tgcgtgataa gctggaaaat aataccatgt atttccgcga aaaaatgaca gcagccggat
     2941 ttgatattaa acccggtgtt catcctatag tgcctatcat gttatatgat gccgtattga
     3001 gtcaacagat ggctgcgaaa ttgttggaag agggaattta tgtgattgga ttttattatc
     3061 ctgtagttcc aaaaggccag gcacgcatca gggtgcagat ttctgctgct catgatcaac
     3121 atcatcttga caaagccatt tcagcatttg taaaagttgg caaagagtta ggggtaatta
     3181 aataattgct gcaagagagt atcacttatt atattttctg ttcacccatc ttggtaaaat
     3241 caccacacca agaagcagat aagggtaaaa tgaaagcaat cgccataata atgcgagggt
     3301 ggctactaat gcggggtctg taaagtcgca catcatggca ctaaaggtta attcggcaat
     3361 acctgcacca ccgggactta tgggcagcaa catgagcatc cagatggcaa acatcctgct
     3421 gaaaattacc ggcagatcgg caagtgttgg gtcgaaaaag gcatggacaa tgcaaatggg
     3481 aatcataaaa aatgctatcc atgtgattgc cgttgatata aataatttcc accaaaaccc
     3541 tttcgattta tgttttaatt cgtgcgatgt aagtatcatg tcgtcacctg tttgcagggc
     3601 atcattacgc cattttttga gtatgggtaa gctgaaaaga gatgacagca gacttttaaa
     3661 tgcatgtgca ttgatgaata agccgtaagc gagcaatgaa attatgattg caaataaacc
     3721 atagccaaga aaatagattg tttgcatgtt atgaaatgcc gacatcatgg gcagttcact
     3781 ctggctgcga cattgtgcat ctgtggcaaa catggcattt cctcctagca aaacaaataa
     3841 aataagtcct ccggcagcaa gaaaaacctg atcaaggaat atagtaagca tgctggtggc
     3901 ggcgcttttt cccaggctta atttttcctt tttcatgaaa taaatagcaa tagccgcacc
     3961 ccctacggtg gatggtgtga tggccgaaga aaactcccac aaagagatca cgttgaacga
     4021 ttgataccag ctcaatttgt tgtcggtaat gagtcgtaat cgatacatat atccaatatg
     4081 gcgcgctacc atcatcagcg caccgaaagc gagccatccg aaagcatcct gagcccattg
     4141 gatgtgggaa atggctttat aatcgaatga agtataaatg agatatgcgg agataccaag
     4201 actcagcgct atgactgcgg ctatgcgcgt gtaggaaaac tgtttgttga tttgtttttc
     4261 ctgctcgttg cccggttgct caatcattga agccatatgc ctttcgaagt tatagaaaaa
     4321 agttgaacat gaatcgttgg cttatttgcc caaggtaaaa caagctgcat atagcgctaa
     4381 ggttgtttta acactttttc ttttgtcaat tatacttcca ttaatacctt taagccttat
     4441 gcacaggttt tatgcatgca tattattact attggcaact tgttcgatga gcagcgggtt
     4501 ggtcatgtac ctgcgcacta cacatgctcg acgggaggtg attgtgcaat attcccgcac
     4561 agaaaaccaa ccggagacaa caattttaaa gctctccggc aacgaattca ctgcacttga
     4621 gaaggaaaga atttctgaaa agatatttga aattcaatgg aagggtgaaa ggtatgatat
     4681 tattgatttg caatatcagc aaaatggcga tgtttacctg accgcgcatc acgacaaaaa
     4741 ggaagcaaag gcaattaaag attttgcaga tgcgcatcaa gatagtgcaa atgccgggaa
     4801 cctcattttc ttctcttttt gcgacggcat tattcacact gatatcattc attttatccc
     4861 aagtgaaatt ttcttgcata gtgaatataa tgtgatcctg cataggcatt ctatttcacc
     4921 cacttctcca cctccggatt ttttagcggc ataaacaatt atgtaacgct aaaacataca
     4981 acatgaccct atttcgaaat gggttattgg tgttggcaac aatgtattta tatgcatctg
     5041 tgtgttttgc acaaacggac acagttacat tggatcgtat cgtagtaacc tcttttaagg
     5101 atacgcgttt acgtgaaagt agccttacca tcacacccat cactttatct gatatcgcac
     5161 aaagaggtgc atttaatatt tctgatgctc tggcaaaaac tccgggccta agtcaattag
     5221 gtacaggaaa tgctatttcc aaacctgtaa ttcgtggttt gtatggcaat cgtgtattga
     5281 tattgctttc gggtatgcga ttcgataatc agcaatggca agatgagcac ggactcggat
     5341 tgagtgaagt gggtatcggc agcgttgaag tgattaaagg acctttcagt ttattacatg
     5401 gaagtgatgc cgtaggcggt gttatcaata tcattgaaga ggataaatcc gatccgtttc
     5461 acattgatca ggatgtttct gtaaaattca atacaaacac acttggtgaa atggtccagt
     5521 atggctataa gcgcaatgat ggcgcacact ggataggcat acgcctggct gcagaatcaa
     5581 gcccggatta cagcgatggc aatggaacga gagtgcttaa cagtcgctat gatggttatt
     5641 atctaaaagg caattatggc tttaagaaag gtaattggat aagcgataat cattacatgg
     5701 gttcctttaa tcgatatgga tttattttca gcgatatccg cgatttcttt gaaacgcccg
     5761 acgcacgatt cagcagatct tttcccggtc cgcatcatat agttgctttg aatatcggca
     5821 cttcggagaa cactatcatt ttacctaaaa gtgttttgaa ggtgaatgtc ggtatacagt
     5881 cgaatgcgcg gctggaagat gaaggcagtg gttcgatcag tttatttatg catttagtta
     5941 cgggtcaata tgctactcga tggactaaat cattaaatca gagaaacact ttgataattg
     6001 cacacaatgc atcaattgaa aacaatacca attacggaaa aaaggttatt gtacctgatg
     6061 cgtggatggc ggaagggaat cttgcggcat atgtatcaca cagttccggt aaattaattt
     6121 ttgaaaccgg aattggcggc ggcgacagat acatcaaaac cttgtatacg gcaggtgtta
     6181 attcgcctga aaaggatatt gatccgtttt cccgtcattg gttgtttgca aatgtcctgg
     6241 cgggattaac ttttaatccc aacaataact ggaatattaa atcgcatgtt gccactggtg
     6301 taagggctcc gaatctagct gaattatctt caaatggttt gcatgaaggt gtatttacct
     6361 acgagattgg cgatccaaat ctgcatacag agaaaaacct cagccttgct gcggatatta
     6421 atcgaaccgg taaatatatt actgcttcga taagcggcta ttacaatttc tttgcagatt
     6481 ttatttattt atcgccaacc gatgaggagt ggtttggttt cccaatttat cgctacttgc
     6541 aacaatcagc tcaagtgtat ggtgccgaag catttatatc tttcagaatt cctgcatttc
     6601 cgtatttaac ctacagcata accactgcag atgttgaagg aaaattgaaa gatggggcat
     6661 acctgccatt tattccggca ctcaaggtaa cgcctgaaat taaattcaag ggattacatg
     6721 atgagcataa ttggtatgca tttgtcaaca ccgatatata ctcacctcaa catcaaacgg
     6781 cgacagatga aatagctaca ccgggctaca cattactgaa tgccggcgtg ggtggcgagt
     6841 ggcatattgg aaaataccca cttgaaatta atctggtggg aaataattta ttaaacgaag
     6901 cctattatga tcatttgagc agatttaaat cgctcggata tctgaatatg ggcacaaata
     6961 ttcaagtgca tgctatcatg cattttcaat caacttttaa atatcacaac aaaacaaatt
     7021 aaacattatg aaacatttta tgaaattgaa tactttgatc atcgccgtag cagcgatcac
     7081 atttgcatta gcatcgtgta ataaagatga aatggaacca ccaatcatta gttttaaaac
     7141 cggcgcagga tatacctcag atgatattac catagctgca ggttcttctg tattaattgg
     7201 gattgaagca tcaaaggctg aagccgaagg tgaggaagag gatgtgttat cacactttaa
     7261 tatttcttta tctgtcaacg gcggagcgtc aacatcagtg tatgatgctg atatgactgc
     7321 tgctcaagaa gaggagtatt catacgatta caatgctgct gcttcaacta ctgttggtga
     7381 agtggacact tacactttca cgattacaaa cagagatggt ttaacaggtc aggtaagttt
     7441 gactgtcact tcgaattaat agtcgcgctt aataaaaaaa aggctgcttt cgggcggcct
     7501 ttttttattt cagaaaattg attttaataa ttagtcctga tcagaagtaa gccagaaatt
     7561 attaattcct tcgccaacca ataatgttac tttttgtgtt gcaataagat caagaatact
     7621 caggaaacgg aacacagcct gatatttgtc tttacatgaa ttgaatacat cggcaaatgg
     7681 aacattcttt ttgccacgaa cgtaagacag cacaccaaaa ctttcttctt caatagaata
     7741 attgtattgc acaattacgt gttcctgtac tttcggctgg ttgttatagc gatccatcac
     7801 cttttggaat accttgagca gcacaaaaag gtttacgcta ctcaattcca tttcattgct
     7861 atgtttttta gcaagaatat cgtgttcgat tggaatattt ccacgattaa ttctcagctg
     7921 acgatcgtct tccagttctg agagatgtcc gagtacttct ttgtatcgtt tgtattccag
     7981 aagtcggtct acgagttctt tacgcgggtc gatctcatta ccaaattcat cgagttcttt
     8041 gcgcggcagc aacatttttg ctttgatacg catgagggtt gctgcaacaa gaatgaattc
     8101 actggcaatc tcgatattca tctgcgtcat agagtgcagg taatcgagaa agtctttggt
     8161 tatgcgtgaa atcggaatat cgtgaatatc caactcatcg cgttctataa agaataacag
     8221 cagatcgaac ggcccttcga actgtggtag tttgatttcg aatgtttgct ggttttgcat
     8281 atgtattggt tgtcaaaggt agtgtgtgtt gcgaattccg gcaagcgact ttctaaaagg
     8341 aaaaaaattg ccggtatcga tgcttgtcac actattaatt ggccatttcg ctcataaatt
     8401 tgatgtgtaa caatcgcact tcttcaaatg atagaccttc atccttcaag tcattgtaag
     8461 cggaaacgag gtcgaggctg ctcatttcta tgaaatagtc gtgcacttct tcctgcagat
     8521 cgctttctat gatatcatcg agataataat tcaagctcaa ttttgtgcct gaggatacta
     8581 ttgtttcgat ttcatcaata aaatcaggga aattcaagcc tattgatttc gcgatatccg
     8641 gcagcggcat tttcttatcg atgaattgga tgatcttcac tttgtgtgca gatttattaa
     8701 ccacctgctt catcacaaat tccgaaggct tttctacatc attttcctct acatacttct
     8761 taatagcttc aagaaacttc ctgccatatt tttctgcttt tcccctgctg acgcctgaaa
     8821 ttttcaccat ttcatccata acagtcggat acatggtagc catttcttca agagagtttt
     8881 cgctgaagat cacataaggt ggcagtccgg cgtctttcgc aactttatcg cgaagcttca
     8941 ccaaaatatc catcatcaca ggatcgagca cactttttgg agcattgctc tcaagcatat
     9001 ctacatcggc cacttcctga tcataatcat gatttaatga aaccatgata gaatgaggct
     9061 ttttgataaa gtttcttccc ttatcggtca attttatcaa gccatattgt tcaatgtctt
     9121 tatacaccaa ttcactcaac aaacattgac gaataatgga attccagaat tgatcgtctt
     9181 tttcttttcc tacgccaaat aattcaattt tatcgtgacg ataattttgg ttttctgttg
     9241 tgcgttttcc ggtgaggagg tcaactacat atttgatatg atgattttcg ttaatcacat
     9301 caattgtttt caaagcattc acaacaaatt ctttgccttc aattttctct tttggtttta
     9361 agcagttgtc gcagctacca caattctctt gtttgtattc ttcgccgaaa tagaagagta
     9421 aaaatttcct tcggcacaca ctcgtttctg cataagccgc agtttccgcc aatagctgtg
     9481 cacccatttc gcgttcggaa aggggtttgt cgcgcatgaa tttttcgagt ttctcgatat
     9541 ccttatagct atagaatgca atacattttc cttccagtcc gtcacgacct gcgcgtccgg
     9601 tttcctgata atagttttcg atagatttag gaatatcgaa gtggatcaca taacgaacgt
     9661 ccggtttatc gatacccatt ccgaatgcga tagtagcaac gatcacctga tatccttcca
     9721 tgaggaattt atcttgtact tcagagcgca gagcagcatc taatcccgcg tggtatgcac
     9781 cggctttaat accgttgatg tttaatatat tagcaatatc ttcggttgat ttgcgattta
     9841 agcaatatac gattgccgat tttctgcctt gctccttaat gtattttacg atctgtttga
     9901 tcgcctgttc acgagaacgt ttcggacgaa tttcataata gagattcggg cgattgaagc
     9961 tggaaataaa tacattcggt tcttccagat cgaggttttt cttgatatcg ctttgcactt
    10021 ttggtgtagc ggttgcagta agtgctatga taggaatatc atcattgata gcttctatca
    10081 tgctgtgtat ctgacgatat tcaggacgaa aatcgtgtcc ccattcagaa atacagtgtg
    10141 cttcatctac agcaacgaaa gaaacgcgta tgccgcggaa aaattcgata ttttcttctt
    10201 ttcgtaatgt ttcaggagca acgtagagca tttttgttct tccggcaacg atatcggcct
    10261 tcaccttttt aatttgctgg cggttgagag aagaattgag aaaatgcgca atgtcgttat
    10321 tgctgctata gctacgcacc aggtccacct gatttttcat caaggcgatg agtggcgata
    10381 caatgattgc ggtaccttcc agcatcatgg caggaagctg atagcacaat gattttccgc
    10441 ctcctgtagg catgatcaca aaagtgtcct ggccattgag gaggcttgta attatttctt
    10501 cctgatttcc tttgaacgaa tcaaatccaa aatactcttg taacgcgtct tttaatgtta
    10561 aatgagatac atccatctgc ttcacgtgtt tttattgact gacgattggg tatatttgcg
    10621 ggcgatttag acaaagcatg gaatatgctg cctaaaaacc ggcaacgaga atcgcctttt
    10681 aaatataaca aattttaatt actttttacc aagtagaatt atatcatccg cgtcaatgag
    10741 taatgtttat gtagtaatca tggccggtgg catcgggagc aggttttggc ctgaaagcag
    10801 ggtcaaaaaa ccgaagcagt tcctggacat cctcaacaca ggagaaaccc tgatccaaac
    10861 cacctatacc aggtttttaa aaatagctga caaagataac atttttatcg ttacgaatga
    10921 acagtatatt tcgctggtaa acgagcaaat tccgggccta aatccgctca atatccttgc
    10981 cgagcctagt cgcaagaaca cggctccctg catagcctat gcctgccatg tgatagccca
    11041 gcgcgatcct gaggcaacag tagtagtggc gcccagcgac cacctgatgc ttaaccctga
    11101 taaattcctg ctgacagtgc tggaagcctt ggagtacgcc gattcacacg atgttttgct
    11161 cacccttggg atcaagccta cccgccctga tacgggctat ggttatatcc agtatgaaga
    11221 ggcttcggta gccggaaaca tccacgaggt aaagactttc accgaaaaac cggatcagga
    11281 attggccaga acctttatta aaagtggcga tttcttatgg aattcgggga ttttcatctg
    11341 gagcgtgaag agcatcatga gtgctttcga cctccatcaa caggacatcc accaggcatt
    11401 ttgggccgcc cgtaaggctt tcggcactaa ggatgagaaa tggatggttg aaaaagctta
    11461 cagcatttgc ccgaatatct ctatcgacta cgccatcatg gaaaaggctg ataatgtgta
    11521 tgttattccg gcatctttcg gatggagcga cctcggaaca tgggcgagcc tgtgggataa
    11581 ctacgataaa gactactatg gcaatgccgt taaaggcaaa aaggtgatga tatacgatgc
    11641 taccaataat atgattatgt ctgatgatga caagttggtg gttgtgcagg gacttgagga
    11701 ttatattatt gttgacacca aagatgtact catcatctgc agaaaagata aagagcagga
    11761 gatcaaagac attacttctg atttgaagcg gaaggaaatg gatgattttc tttgagtgcg
    11821 aacacagctg tcagatctat aatatttaaa taatatcatt accttctcgg gaagcgaata
    11881 ttattttgag gcatgtgaag taacgcaaaa tcaggaaagt gttttgcagt cggcatcaat
    11941 taatttcaat aactgctaat aactgcttta attgtgtaga gaattcaaat ttttctatta
    12001 cacgtttgcg tcctgcaatc ccatattttt tgcataaatc ttgatcttct gcgaattgtt
    12061 ttattgctaa tgcaaaggca tcggtgttac cacatgggac aagcaaacct gtttcgttat
    12121 cagcaatgat ttcaggatta ctgctgatat cgtaagctac tacgggaagc tcatgcgaca
    12181 ttgcttctgc gatcacaaat ccaaaacctt cccaatgcga ggatagcaca aagatgtcga
    12241 tactttgcat gaaggcaggg atgtcatcaa caaaacccat cagaaaaact tgattctgta
    12301 attgatgcag cttgatggca ttttcaagtg atgtgcgttc ttctccttct cctgcgataa
    12361 agagcgtaaa gtttatctgt agatctctga gtttaatggc tacctcaatg agatcttttt
    12421 gcgctttttg tgcagttagt cggccggcgt tacccagaat tattccgttg ccatgttgcg
    12481 caataaatgg gtgtttattt cgcgattgag tttctacaat attaatacca tgataaacaa
    12541 caggtatgtt cggaattcct tttacatgtt gcattttttg aagaagcagc gattttgttt
    12601 cttcagagtt tgctataata tgcgttaatg tttttgtgaa taggcgtctg ttaaagtagc
    12661 tgtttttaac tcgtgcagct aatccgcgca gataaacaat tttgtttatt ccggctttct
    12721 ttgcagcagc gcctcctgtt ttagcatctt gcgaagcagt aaaaatgata gcatttgcct
    12781 gaatgcttgc aagtgtattt gagattttat ttatgtggaa tgtatttaaa aaggagaatt
    12841 tagttacacg tacatccacg cattccaacc cggcctcttg cgcgcgttgc cacaaaatac
    12901 catttttatt acaggcaatt acaacatgat gtccaagggc tttgcatcca acagcaagtt
    12961 cgagatgtaa cttttccccg ccaccccaaa agcgtattga attaaagaag caaattgtag
    13021 ccacgaggta aaaataatgg aattcaagga tttggtaccg tatgaattga atcaaggggt
    13081 ttggatgcta tttattgata catttatttc cattactttt atcccatggc cgatcataca
    13141 gtacagatca agagtaaatt gcccaatgcc ggaatttcca tttttgcccg gatgacagca
    13201 ttggcaaatc agcatggggc gatcaatctg gcgcagggct tccccgattt tatgtcgagt
    13261 gaaaaactta tttccctggt acatgcatat atgcagaagg gaatgaacca atatgcgcca
    13321 atgcccggtt tgcttgcatt gagggaaaga attgcggaga aaattaaatt tatatatcac
    13381 aatacttatg atccggcttc ggaagttacc atcacggcag gagcgacgca agcaatttat
    13441 acaagtatag cggcaaccat tcgcgacggt gatgaagtaa tcatttttga accggcttat
    13501 gatagttatg ctcctgcaat tcgtgtaaat ggcggaattc ccgttccggt ggaattaaaa
    13561 gcacccgatt ataaaattga ctggaatatt gtaaaaaaac ttgtctcgca acgcacgcga
    13621 atgatcatga ttaatacacc tcataatcca acgggtacag tcttggatca aagtgatatt
    13681 aagcagttaa ttcgtattgt gcacggaaaa gatattattg tattaagtga tgaggtatat
    13741 gagcatatta tttttgatgg catcaggcat gaaagtgttt tacattatcc ggagttgaga
    13801 gaacgaagta tggtcatatt tagtttcgga aaaacatatc acaataccgg ttggaaaata
    13861 ggatatgtat tagcgcctga atatttgact aaagaattca gggcagttca tcagtttgag
    13921 gtgttttcag tgaatacacc cattcaatat gcgctttccg actttatgga agatcgcagt
    13981 gagtatgaaa aagtcagtaa cttttatgct gcaaaacgca atttatttag cgatatgtta
    14041 aagggttcga aattcgtttt aaaaccgtca gcgggaacat attttcagtt gttagattat
    14101 agtgcaattt ctgatctggg tgatcaggaa tttgctgatc tgctgactat agaacataag
    14161 gttgctgcaa ttccactatc tcctttttat agaaatggga gtgatgcaaa aatgttgaga
    14221 ttttgtttcg ctaaacaaga cagcacactt caaaaagctg cagatatatt atgcaagatc
    14281 tgaaagtcac acttgttcaa gtagacctgg cctgggaaaa taaacaggca aaccttgatt
    14341 atatttctac attgctaaaa gatgttcggg acacagacct aatcatatta cctgaaatgt
    14401 tcagcacagg attttccatg cgatcgaaag atctggctga aaatatgatg ggtaattctg
    14461 tgaggtggat gagggatttg gcaagtgcga aaaatgctgc agtgtgcgga agtttgatta
    14521 ttgaggacga tgagaaattc tacaatcgtt tgatttggat ggagcccgat ggcacgagtg
    14581 cgcattatga caagcgacac ttgttttcat tggcaggtga agacaagcat tataccggtg
    14641 gaaagcataa gatattccct gaattgaaag aatggaagat attgccattg atatgttatg
    14701 atttaaggtt cccggtgtgg agcagacaat ctccaccgtt taccgagttg ggagccaatc
    14761 cttatcacct gctggtgtat gttgccaatt ggccggagcg aagggtttat gcgtgggagc
    14821 agttgctgat agcccgtgca attgaaaacc agtgttatgt tgtaggcgtc aaccggatcg
    14881 gtatggatgg caatgacgtt taccataacg gctgttccct ggtagtagac cctatggggc
    14941 agaaactata cgagtcgaaa ggcgaagaat ccgttaaaac acttacactg agcgggttac
    15001 agctcgaatc gatacgagaa aaactgccat ttcttgatga tcgggatgag tttgagctaa
    15061 ggcgttaatg ttaaattttc acaaaatcat acaatttgtt gggaattctg tcgtcatcac
    15121 tgatatcttt gtgatatcat tttgaaatct aaaactaaaa ctatgataac cgacaaaatt
    15181 gtgaaaccga gttccggctt ctttatgttt tttgtattcc tgttaatgag tataggcatt
    15241 gggtatgcga tgatgaatgt gcatccggct tatagtcttg cttttattcc tttgtttttc
    15301 gtgttgattg gctttttcat tatcaatccg aatgatgcca aggtgcttgt attatttgga
    15361 aagtatgcag gcacggtaac ggagaacgga ttttattggg cgaatccttt ttatggcaag
    15421 catcgtgtat cgcttaaggc gaggactttg gatggcgaca agatcaaagt aaacgacaag
    15481 ctaggtaatc ctatcattat cggagcagtt gttgtttggc aagttgatga tgctgcgaag
    15541 gcaaaatttg aagttgagaa ttatgaaatg tatgtaaagc tgcaaagtga taccgctgtt
    15601 cgtcacttgg cgggttcatt ttcttatgat cattttagtg ataatgatag tgaggagatt
    15661 accttgcgca gtggaggtga gcacatcaac gaaatgttgg tgaaggaatt aaaagatcgt
    15721 ttgcaattgg caggtgtgaa agtgattgaa gcgagaatca cgcatttggc ctatgccgaa
    15781 gaaattgccg gtgcgatgtt gcaacgccag caagcaactg cagttgttgc ggcaagaact
    15841 aaaattgtgg aaggagctgt aggcatggtt caattagcat tgcagaaatt atcgaaggag
    15901 cagattgttg agcttgacga agaaaagaaa gcggcaatgg tgagtaattt attggtggtg
    15961 ttgtgttctg ataaaacagc gaatcctgtt gtaaacaccg gtacattata tcattaattc
    16021 agtgttatgg aatttcagga agagcaacga atcagtgtaa tgtggatgcg catattattt
    16081 ctgattataa taatcggaag tctggccggc gttttacttg gcggcatgaa cagtggccat
    16141 gagccccaga tttccgatta cctgggagct gtattttgcg ttgcaatagt ttcgttagtg
    16201 tattacatgg ttttttatac taaactgaaa acatctgttt ctatgatggg ttttgcatat
    16261 gaatattttc catttgtatg gcgagagaaa actattacga aggaaatgat caagagttgg
    16321 caaatgcaga caatgaagag tggatttcag tatgggggat atggtatcaa gcagagttat
    16381 ttccctaaaa aaaccgcatt tatgatggga tctcgtaatg cagtagagtt taagttgcat
    16441 gatgggaaaa cattaatttt tactacgaat aatccggagc agttgcaatt ggcgttgcga
    16501 aaatattttt cacagacaga aattcgttga tggcaaaaga agacaataaa tcggcaaaag
    16561 atcgtcaggc ctttatattg cgggtgtcgc ctgaattact gaaggctgtc gagaaatggg
    16621 ctgccgatga tttcaggagt gtgaatggtc agattgaatt tttattgcat gaggcattaa
    16681 agaagtcggg acgcatgaag aagcgtattg aagaataaat tttattctgt gaatacgcgt
    16741 tctgcctcgc gagattgaat aaacgcctta tgaaagtcaa tgcatttggc tttgcctccg
    16801 aacagcggca gcacgttcga ttgaatagta ttatgttcgc tgctgatagc gaggtgatat
    16861 gaagtgaatc gccattgttg aaatttatct accatgccat gatgttgagc gttgatatgg
    16921 atgtaagcag tcagatcctg aagatgtatt gggaagaagt ggtgatgtcg taaatttctc
    16981 aggatatatc tgggatgtaa aaattgccga atcataccag gtagggcccg gtccaaatta
    17041 tttttcagga tattatgaag atgtttttgt cgatgagcaa ggatatttac atatgcgcat
    17101 tgccgaacac gatggcaaat ggttttccag tgaggtggta tcgcaaatga acatgggtta
    17161 tggaaaatat tcatgggttg ttcaaggcga tcttgaaaat attcctgaaa atatcacgct
    17221 gggtttattt acctgggata acaatacttt ttttgatcag gcgaacagcg aagtggatat
    17281 cgaattttca aaatggggcg tggcatctga agaaaatacc atgttatata gtgtgcaacc
    17341 ggtgaatttc ggaccaacct atcctgaacg cacacattta atagaaactg aagttggcga
    17401 tttggtgggt gttaccacac atacttttat ttggaccgat acacttatca cctggaatag
    17461 ttattatggc gatactgaat ctgaagataa gcgtatcggc acctggtatt ttaatttgga
    17521 taatccgcca agagtaaaaa atgaaaacgg aaacagctct gcggctatcg ttattcctgc
    17581 gcccggagaa acaaccaatg cgcgtatcaa tttctggtta ttgccatgga tttcatcgtc
    17641 gccaactgat ggactcgaac gtgaaatagt aatcagaaaa ttcagctacg aggctgctga
    17701 ataatgataa ttaatggcag cacttatttt tcgaacaacc ataaataagc tgctgaaaac
    17761 cccttcctcc aaaaggattc tgtatgtatg ccattgcctt ctatcactat gcgcaacgtc
    17821 aaattattaa tttgcttttt ttgtaatagc ctgtacatcg ctatcgcatt ttcttgcatg
    17881 tcatcatcat caccctccct gccacctgcc aggtaataaa tagctgttgg cttgtctgac
    17941 gtataagcct ccgctaattg gaaattttgc ttgctgatcc agaaggatgg tgagaaaacc
    18001 aatgcatttc caaatacatc attatactgc aacaccagat actgactcat aaccccgcca
    18061 acactgctac cacctattcc tgtatgtatt ctgtcgggga gcgtgcgata gaggctatcg
    18121 atatgcggtt tcaactcctc aacaacaaat tgtgcgaaca aatcaccctt tccactttca
    18181 ttaaatcgcg ctaccttcca aggtgtatac tctgcaagcc tcgcatctcc cgcgtgatcg
    18241 atgccgacaa taattgcagt tgtctcgccg agatgacaaa tagaatccat cgattcatca
    18301 atgccccatt ctcctgcata tgataattga tcatcaaata aattctgacc atccaacata
    18361 tacaatacgg gata
//
LOCUS       DAOQHY010000052        18361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1400_length_18361_cov_4.659893, whole genome shotgun sequence.
ACCESSION   DAOQHY010000052 DAOQHY010000000
VERSION     DAOQHY010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 18361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18361
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1400_length_18361_cov_4.659893"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PLL28_06035"
     CDS             complement(<1..453)
                     /locus_tag="PLL28_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNF68917.1"
                     /translation="MKRLRIPINIPEEQLIRQCLQGDRVSQKHLYDRHSSKMFGVCLR
                     YAPDYQAAEDILQEGFIKAFRNLERFRFEGSFEGWLRKIMVNTAIEVHRRKNSLYPIM
                     DVEHTDVEFFDENALDKLAAADLMQMIDTLSPGYKTVFSLYAIEGFSHK"
     gene            complement(503..1120)
                     /gene="rdgB"
                     /locus_tag="PLL28_06040"
     CDS             complement(503..1120)
                     /gene="rdgB"
                     /locus_tag="PLL28_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNF68918.1"
                     /translation="MGAITDPVRIEMSDLIFATANENKVKEVQLMMPSGFAIKGLRDI
                     GCLEELPETTGTIEGNSRQKAMYVHEKYGVDCFSEDTGLEVAALDGEPGVDTAHYSGS
                     RDPEANIRLLLMNLRNEGNRAAQFKTVFTLIIGKNVHQFTGIVTGKIAEIPTGNMGFG
                     YDPVFIPDGYSTTFAEMKTEEKAPISHRGKAFAAMTAFLTQNFWK"
     gene            complement(1084..1560)
                     /locus_tag="PLL28_06045"
     CDS             complement(1084..1560)
                     /locus_tag="PLL28_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68919.1"
                     /translation="MLEFGGAMVDEPVFLVSDATKLTISPTKANDEGLDLSNEVHSLF
                     LFKPSSGNRLSPDAMIALDKMIAACGLTKENAVALPFLGQGNLHFNRLASAGYVKKLI
                     LLGITPDEIHLFVDSQYYNIFNFNGIDMLISDNIEGISKEQKQQLWARLQTLFGLK"
     gene            1626..2705
                     /locus_tag="PLL28_06050"
     CDS             1626..2705
                     /locus_tag="PLL28_06050"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HNF68920.1"
                     /translation="MDTAINTTFPIEIHKSPVSHISTVDFDQLQFGKVFTDHMFEADY
                     IDGKWTNCTIRPLSTLQLHPATSALHYGQAVFEGMKAFKTTEGKVMLFRPDANFRRLN
                     ESCVRMAIPELPEELFMDALFQLVRIDADWIPNTDEGSMYIRPFVFATDDFVGIKPSD
                     TFKFIIFCCPVARYYSKPVRVYMQEQFIRAFPGGTGAVKAAGNYGATMMPLMEARKRG
                     FDQMLWIDGVTHNRLQEIGTMNVFVQIDDTIITIPTTEGTILKGVTRDSCIALLRDGG
                     YKVEERDVTIQELIDAHRSGRLHDAFGTGTAATVAPIGVIGYKDEEYELPPVESRKAS
                     AWLKHELWAIRKGIATDRFDWMWEV"
     gene            complement(2766..4766)
                     /locus_tag="PLL28_06055"
     CDS             complement(2766..4766)
                     /locus_tag="PLL28_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF68921.1"
                     /translation="MIIRSLCLLLFSVTCLSSFSQSADIIWAKTVVKCTSQKSDKLFA
                     SSQALGAPNRYPNPSYTTCAWSPEVSDSRDSEEIHVKFDTAVIAKQILIAESANAGHI
                     RSIWVYAPDGSQRKAFERTTPSGDMPGRMFVVNLKGNTKQVNAIRLVMDKPTLYNTYQ
                     IDAIGLSAAENAILPTLRLAETPFPGKAERLPDGINSAYDEVYPLVAPDGKTLYFDRK
                     LDPANIGEQHADDIWISTNTGTSWSTAINLGPPLNNKNHNFMCAISPDGNTAVVGNTY
                     GAEVTNTGISLTKRSSDTWTIPEPIVITGFENLNQYNEFSMSASGNILLMSIEKADGM
                     GLLDLYVSFRQADGSFGVPKPLGVDINTAGSEMTPFLAADESTLYFSSNGFPGYGNQD
                     IFVAKRLDDSWQRWTSPVNLGPEVNTPEWDVYFSIDADGDYGYFSSSKNAITNLDIYR
                     IQLPPELQPDNILWLKGNISDRITGQPLKAMINYESTGVRTGTGTSGEDGNYALVMPG
                     ATDYHVLVSAKGYLTADTIIDLKSLLADKQAIQDFELVAMQEGIVIKIPNILFKVNSA
                     VLEDTSFIELERIARLLINNPGISVEIRGHTNGMCDDTYCKSLSTKRAESVVKYLVSK
                     GISPERLTSKGYGKSMPVADNTTPEGRQANQRVEFMITKVGD"
     gene            complement(4772..6934)
                     /locus_tag="PLL28_06060"
     CDS             complement(4772..6934)
                     /locus_tag="PLL28_06060"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF68922.1"
                     /translation="MRKLHTLLFLFILAGYTCSAAEIMWASKVISYSSQLDLNSYSAK
                     QVLGPPSRLPNFGDCGCAWSPSMSENFFEEYIRVGFDKRIRVSQIVINESFNAGAIKA
                     IYLFDQYNIPHLVWERPAEGNKWELGRVFSINITPTDFASNDLKLVLDTESIDGFNQI
                     DAIGISEAPIEIPRGSINVTDKVTFKGKSQNLGPTINTYGSEITPLLTPDGKTMYFTR
                     KNHMGNTGTIMNDDIWVSTYDGTKWTEATNIGGPMNNENNNYVVGISMNGEMLTLANT
                     YSPSGESLIGIAQTWKTEYGRWVFPKNLITPGVITHGWFAEYFMNSDRDVLLLSLERA
                     DSHGMKDIYVSFSDNQIEWTEPINLGTDVNTASSEMAPFLGPDGKTLFFSSDGLPGYG
                     DQDVYVCVRLDSTWKHWTKPENLGSLVNSKGFEAYFTYPDTADYAYFSSTGDDKLNAD
                     IYRIPLKEVKEIEDSVLAEILSDVVLTSGDSTVADIITPDKPADPGEILFEEEVVVPE
                     KEALNITLTNEILLFGTVFDANTNVPINAKLSFVLTDYESDPIDMNTLNNTYRLKVTD
                     SVQYKVTVIREGYLPLEMTINIEDFRTQKVKRVDFKLTPLHSGEKIILDNLYFDANKS
                     IIKPESFEELNRLYDFMIANPGLIIEIGGHTNGLCSEAYCEKLSLNRANAVREYLQNK
                     GIDPVRISTVGYGSSQPIDTNSTPEGRKRNQRVEVTFK"
     gene            7060..7689
                     /gene="nth"
                     /locus_tag="PLL28_06065"
     CDS             7060..7689
                     /gene="nth"
                     /locus_tag="PLL28_06065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNF68923.1"
                     /translation="MTRSEKAQFILSKLNELFPETPVPLQHKDAYTLLVAVVLSAQCT
                     DERVNQITPKLFARADNAYDMVKVPVQEIHDIIKPCGLAPMKSKGIHGLSEMIVTKHH
                     GQVPSTFAELEAMPGVGHKTASVVMSQAFDFPAFPVDTHIHRLAWRWGLTNGKSVEQT
                     ERDLKRIFPENTWNKLHLQIIYFGRKYCPARGHDREKCPICSVVGRKLM"
     gene            complement(7739..13246)
                     /locus_tag="PLL28_06070"
     CDS             complement(7739..13246)
                     /locus_tag="PLL28_06070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNF68924.1"
                     /translation="MKKHLTLLLSCMLTLAFMAPTRDAIAGGDNNYYIYSNAYGGYEP
                     WYTTSNSDAMNAVFGVGGWLQDYFETCDPALVFSANTNFVFLEGSDGFADELESFLSV
                     NIDVIETWVEGGGRLLLNAAPNEGDGMSFGFGGVNLTYWGGSSNVHATDPTHPIFNGP
                     YTPTGTSWTGSSFSHASVSGPDLTPLIIDDFTPTTIALSEGRYGTDGIVFFGGMTTDN
                     FHYPSLESINLFQNILNYLAFYEIPDHDIASLSLASPSSGCGMGVETVSVNYKNYGTN
                     DEVNIPVSYQLDGGAVVSATIAGPVAPGETVTYSFAVPADITALGAHTFVISCDLATD
                     EIPANNTITVTINNIPVVDVFPYYQDFEAGDGGWIAGGGASSWELGSPAGFAIVGPPP
                     ATPGSVNSWTTSLTGYYNDGEKSYIESPCFDFSSLVFPYLELDLNWDTQMYSDGAKIQ
                     YSTDAGATWSDLGDVGTGENWYTSYNCYGMWPDFYVTDYRGWDGNSGGWQHGYQDLAF
                     LGGQPSVKLRMVMGADLYWSYYDGFAFDNVKISDLFPNDVGVSNITAPVSGPLLSATE
                     AVSVAVTNYGTLAQSGFPVSYRFEGGPVHTETFTGTVNPGETVNYDFATTEDLSVLGD
                     YTFEAWTSLATDEDMSNDSITEVISNLEPLTGDNAYYIISNTTGGEPWYTVSNSQSMD
                     AVFGVGEWTMGYFETVDPAVVFGTGTCFVFLEGSDGHALELKSFLDANHTAIENWVAS
                     GGHLLLNAAPNEGSGMDFYFDGVELSYPYFSNTVGTDVPTHPVFTGPFTPTSTVMYGS
                     SYGHATVSGDDLSVILYDYYNPSKIVLAEKSWGAGTVLFGGMTVTNYHSPLPDADNFR
                     RNAISYLATCTISDNDMATTAILSPESTCGLTGAETITINVKNVGFLSQTDVPVSYQV
                     DGGTIVTETIPGTIDVGEIVTYSFATTVNMAVPGDYNIVTWTDLATDTIYGNDTTYST
                     VYNVPTVASFPYYEDFEDGSKGWLVGGASTFELGTPAGYVINTPPTATPGSLNSWVTN
                     LEGYYDNNEKGYVTSPCMDFTSLVMPYMELDINYDIQLYSDGAKIQYTLDEGASWSDL
                     GNVGEGENWYDSYYCYSMWPTFYVSDYRGWQSTYTGWRTAHYDMSFLAGQPSVRFRII
                     FASDSYWNYNDGFAFDNVKIQDPFPNDVGVVDLISPESAVTLTGSEVVSVLLENFGTL
                     PQTGFNVAYKVGAGTVHTELFTGTVDPGSTAVMTFAATEDFSADGLYNVTAWTELASD
                     EDLTNDTLNAIVVNLTPVSGTDAFYIYSNVYGGYEPWYVTSNSDAMDAVFGDDGWFLE
                     YVETLDPYAVFDENTCFVYLEGSDAQASELENFLTTNSSIVENWVASGGHLLLNSAPN
                     EGDGMDFGFDGVTLNYSWYTSNVTAYDPSHPIFNGPWTPITTDYTGGSFGHASVSGGG
                     IDPVIIDTYDASRYVLAEKEWGDGHVIFGGMTPPYFHSPSPDAQNLLQNIYETIKLCA
                     PVDLGVVEIISPEGGCGMGVETITVTVENFGPSTVSTFPIKYQVDGGAIESDFATVTI
                     TPGGTATYTFDVSYDFSVPGTYDLCVWTDFAGDSDEGNDMICVTITSLATPSVDLGPN
                     MTVCDLVTLDAGNVGSIYSWSTGESTQTIDVTESGTYSVTVTNPTTGCSVTDEVTVTV
                     NYTPEASFTYSASGLTVTFTNTSTDGASYSWSFGDAGTSTTINPSHTYAVTGMYTVSL
                     TVTNPCGSDTYSMVVEVGNGVNDITLAEAVEITPNPTSDYATVQVSLPVSADIQFKLT
                     DYLGQEVWSANMTNSLSATYVIDMTKFAAGVYQLNIISDDAVVGKQIVLTK"
     gene            complement(13430..18358)
                     /locus_tag="PLL28_06075"
     CDS             complement(13430..18358)
                     /locus_tag="PLL28_06075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNF68925.1"
                     /translation="MPFTALPGTTRMRVMCVYFPALAIDPCGVVETYTFGEVEDYGIN
                     IPAAGPYDLGVTSISGLGTGCGLGVTPITVTVTNLGTEPSGAFSVAYQVDDPTLGALA
                     PVVQAYTGAAIPSLGSVAFTFTTPADFSNLGEYTLQAYTIYGVDMVAADDTSDETFTN
                     IPIVTTFPYYQDYEDGAGGWISGGTESTWELGDPAGFAIIGPPPATPTSLNSWTTELT
                     DYYNLNEKSYVISPCLDFSSLIIPYMEFDANWDIQLYDDGAKLQYTTDEGLTWNDLGG
                     IGTGDNWYNTNTCYAMWPTFYIDSYNGWSGNSGGWVHSKHDLSFLAGEPSVKFRFVFA
                     SSSYWNFNDGFAFDNMWIGDPLPNDIGVTNVISPETSPFLSATESVTVEVTNFGTNPQ
                     TGFPVSYQVDGGTIHTETFTGTLGVAEVATFTFSATSDLSADGDYDFVAWTGLASDED
                     VTNDTWSETISNIEPITGTNAYYIYSNSTGFEPWYTTSNSQHMDDVFGVGEWTTEYYE
                     TIDPAVLFSLDNCFIFMEGSDSHADAMETFINANMDAMQLWVAAGGHLLINAAPNVGD
                     GMDFGFDGVHLDYPWYASTVSASDPTHPVWDGPFVPTSTDMTGFNYAHASISGAYLSP
                     ILFDAATPSRIICAERNYGAGTVIFGSMTVTDFHTPLTEAGNFRKNLIAYLATCTISN
                     DDVGVYSIESPETGCGMTDEEIVTIKVKNYGFLPQTDVPVYYQADGGTIVSEVVPGTI
                     GVGDVVLYTFATPVDISLIGDHTLNAWTGLPADTINDNDTTYSTLANIPVITTYPYYE
                     NWELGDEDGWMAYGASSTWELGYPDGPVINEPPTATPGSQYSWLTSITGNYNDNENSY
                     LEGPCFDLSSLVIPYVEVDLWWETEDTWDGMQLEYSTNGGVTWSVLGDIGTGDNWYTN
                     TAFALDYETGWVGSGGGWVTSHHDISFLAGETDVKFRFKFSADGIINFDGIGLDNFKV
                     QDPWPNDVGVTDLITPVSGVDLTTTETVSVTVKNFGTLPQSGFPVNFQADAGTIHTET
                     FTGTLTAGASATFTFAATADLSAEGLHEICAWTSLATDDDVTNDEIPGCADVMHLAPV
                     SGTGAYYIYSSTVGFEPGWMNSNSTAMDEVFGDDAWTLDYYEDIDPYTVFSESNCFIF
                     LEASEGHWSEFESFFEDNSDLIQGWVASGGNLFINASPWEGDGGDVGFGGVSIVYPYY
                     TYQTAATDPTHPIYFGPFTPTTSDFTGFYVAYARTTGDVTPIMNDFYCDDCYILSQKD
                     WGDGRVFFGNMSAPEYYSPSPDNQNLRQNIIDYLKLCAPVDLGVTTLLSPDGGCGTTA
                     TETVSVIVSNFGPSTVTNIPMKFQVDGGAIVTEVADGPVPIGGTYTYTFTATANLATP
                     GTHTLATWTDFSGDEDETNDTLISSIVSLATPVLDLGPNTTVCDDVVLNALNVGSTYL
                     WSTGETTQTITVTTSGTYSVTVTNPTSGCSITDNITVTVNYTPVANFSYTVTGGTVTF
                     TNESTDGASYNWSFGDGGSSTTANPSHAYATAGMYTVTLTVTNGCGSDFYSVVIEVGT
                     AITDMELDQSVTVYPNPTDGRTNVNIALSEAQNITLELLNQIGQTVWTNNPGNILTES
                     INIDMTNMAAGVYNLKVTGDNGTATKQIILTK"
BASE COUNT         5257 a         3997 c         3957 g         5150 t
ORIGIN      
        1 tttatgtgaa aatccttcta ttgcataaag gctgaaaact gttttatatc cgggcgacag
       61 ggtgtctatc atttgcatta aatctgctgc agccagcttg tcgagggcat tttcatcgaa
      121 aaattcaaca tcagtatgct ccacatccat gatagggtag agtgaatttt tgcgacggtg
      181 aacctcaatt gccgtattga ccatgatttt gcgcagccaa ccttcgaaac ttccttcgaa
      241 acgaaaacgt tccagattgc ggaaggcttt aataaatccc tcctgcaaaa tgtcttccgc
      301 tgcctgataa tcaggcgcat aacgaagaca aacaccgaac attttggaag aatggcgatc
      361 atacagatgc ttctgtgata cgcgatctcc ctgcaaacat tgtcggatta gttgctcttc
      421 aggaatgtta atgggtattc gaagtcgctt catatttggt caatttggcg cagtaattca
      481 ggcgtaagtt attaaaaatt cgttatttcc aaaaattttg tgttaaaaat gctgtcatgg
      541 cagcaaatgc ttttcctctg tgactgatgg gcgctttttc ctctgtcttc atctccgcaa
      601 acgtagtgct gtagccatcc gggataaaaa ccgggtcgta accaaaaccc atatttccgg
      661 tgggaatttc ggctattttg cctgtcacaa ttccggtaaa ttggtgcacg ttttttccga
      721 taatcagtgt aaaaactgtc ttgaattgtg cagccctgtt gccttcgttt cttaaattca
      781 tcaaaagcag gcggatattg gcttccgggt cgcgggaacc tgaataatgc gcagtgtcta
      841 ctcccggctc tccatccaac gcagctacct cgagtccggt atcctccgaa aaacaatcaa
      901 caccatactt ttcgtgcaca tacatggcct tttgcctgga gttcccttca attgtcccgg
      961 ttgtttccgg caattcctcc aaacaaccaa tgtcgcgaag tcctttaatt gcaaatccgg
     1021 aaggcatcat gagttgcact tcctttactt tgttttcatt tgccgttgca aaaattaagt
     1081 cgctcatttc aatccgaaca gggtctgtaa tcgcgcccac aactgctgtt tttgttcctt
     1141 ggaaatacct tcaatattat ctgatataag catatctatt ccattgaaat tgaatatgtt
     1201 ataatactga ctatcgacga ataaatgtat ttcatccggc gtaatcccca gtaaaatcag
     1261 ctttttgaca tagcctgctg aggctaatct gttaaaatgc aaattacctt gcccgaggaa
     1321 gggtagcgct actgcgtttt cttttgtaag tccgcaggcg gctatcattt tatcaagtgc
     1381 tatcattgca tcaggactaa gccggttccc ggaactcggt ttgaagagga ataaactatg
     1441 cacctcattc gacaaatcca ggccttcatc attggctttt gtgggtgata ttgttagctt
     1501 tgtggcatca gagaccagaa atacaggctc gtcgaccatt gctcctccga attctagcat
     1561 acttgggcga ttgattttgt aaaattaagg cctttaacct attgtttaac caagttttca
     1621 acattatgga cactgctatc aatactactt ttccgatcga gattcataag tcgcccgtat
     1681 cacatatcag cactgtagat ttcgatcagc ttcaatttgg caaggtattt accgaccata
     1741 tgtttgaagc ggattatata gatggaaaat ggacaaattg cactattcgt ccgctttcta
     1801 cgctccagtt acatcctgca acatccgctt tgcactacgg acaagcagtg tttgaaggaa
     1861 tgaaagcttt caagacaaca gaaggaaagg ttatgttatt caggcctgac gccaatttcc
     1921 gcaggttgaa tgaatcttgt gtgcgtatgg cgattccgga attaccggag gaattattta
     1981 tggatgcctt gttccaattg gtgcgtattg atgcagattg gatcccaaat accgatgaag
     2041 gttccatgta tatccgtccg tttgtgtttg caacggatga ttttgtaggg ataaaacctt
     2101 cagatacatt caaattcatc attttctgct gtcctgtagc gcgatattat tcaaaaccgg
     2161 tgcgcgttta catgcaggag caatttatac gtgccttccc gggtggaact ggtgcagtaa
     2221 aggctgcagg caattatggc gctacgatga tgcctttaat ggaagccaga aaacgcggat
     2281 tcgaccagat gctttggatt gatggcgtaa cccacaacag attgcaggaa ataggcacta
     2341 tgaacgtgtt tgttcagatc gacgatacca ttattaccat accaactacc gaaggaacca
     2401 tcctgaaagg tgttacacgc gattcttgta ttgccttgct tcgcgatggc ggttacaagg
     2461 ttgaagaaag agatgtaact atccaggaat tgatagacgc tcatcgcagt ggtcgcttgc
     2521 acgatgcctt cggaacagga actgcagcta cagtagcgcc aattggtgtg atagggtata
     2581 aggatgaaga atacgaactc cctccggttg aaagccgtaa agcttctgcc tggttaaagc
     2641 atgagctctg ggcaatacgc aaaggaattg ctacagatcg ctttgattgg atgtgggaag
     2701 tgtaaatttc tgcgaatcca attcaacacc gtacctgatt ttcctgaaag gtgtcggaaa
     2761 gtgtgctagt cgcctacctt ggtaatcatg aattccaccc tctgattggc ttgtcgaccc
     2821 tcaggtgtcg tattgtcggc aacaggcatg gattttccgt atcctttgga tgtcaaacgc
     2881 tctggagata ttccttttga taccaggtat ttgactaccg attccgctct ttttgtgctc
     2941 aatgatttgc aataagtatc atcgcacata ccgtttgtgt gacctctgat ctctactgaa
     3001 atgccgggat tgttaatcag gagtcgtgca atgcgttcga gttcaatgaa tgaagtatct
     3061 tcaagaacgg cactatttac cttgaacagg atattcggta ttttgataac tataccttcc
     3121 tgcattgcca caagctcaaa atcctgaata gcctgcttat ccgctaagag cgattttaaa
     3181 tcaataatgg tgtctgctgt taaatagccc tttgcagaca ctaatacatg ataatcggta
     3241 gctccgggca taaccaaggc atagttccca tcttccccgg aggtcccggt accggttctc
     3301 actccggtcg actcataatt tatcattgcc ttcaacggct gccctgtaat gcgatcactg
     3361 atgttccctt tcagccataa aatgttgtcc ggctgcaact caggaggcaa ttgtatgcga
     3421 taaatgtcga gattggtgat ggcattttta ctgctgctga aatacccgta atcgccatca
     3481 gcatcaatcg agaaatatac gtcccattca ggtgtattta cttcaggacc gaggtttaca
     3541 gggcttgtcc aacgttgcca gctatcatct aatcgttttg caacaaaaat atcctggttg
     3601 ccatatcccg gaaaaccatt agaagagaag taaagagtcg actcatcagc tgccagaaaa
     3661 ggtgtcattt cactacctgc cgtattgatg tcgacgccga gcggtttagg cacaccaaat
     3721 gagccgtctg cctgcctaaa actcacatac aagtcgagca aacccattcc atcagccttt
     3781 tcaatcgaca tgagtaaaat atttccgctt gccgacatgc tgaactcgtt atattggttc
     3841 aggttttcaa atccggtgat tacaattggt tcgggtatag tccaagtgtc ggaacttcgt
     3901 ttggttaaac taataccggt atttgttact tcagcaccat aggtgtttcc caccacagcg
     3961 gtattgccat cgggggagat cgcgcacatg aaattgtgat tcttattgtt caaaggcggt
     4021 ccgaggttaa tggcggttga ccatgaggtg cctgtattag tgctgatcca tatatcatca
     4081 gcatgttgct caccaatatt cgctggatcg agttttctat caaagtacag tgttttacca
     4141 tcgggagcaa ccaaagggta tacttcgtca taggcagaat taatgccatc aggcagtcgt
     4201 tctgccttgc cggggaaagg tgtttcagct aatctgagcg ttggcaggat ggcgttttca
     4261 gcagcactga ggcctattgc atcaatctga taggtattgt agagagtagg cttatccatg
     4321 acaagccgga tagcatttac ctgcttggtg ttgcctttaa gattcactac aaacatcctg
     4381 ccgggcatgt cgcctgaggg tgtggttctt tcaaaggcct tgcgttgaga gccatcaggt
     4441 gcataaaccc aaattgacct gatatgaccg gcattggcgc tttcggcaat taatatctgc
     4501 ttggcaatga cggcggtatc aaattttacg tgaatttctt ccgaatcacg agaatcgctg
     4561 acctcgggcg accaggcgca ggtggtatat gaagggttag ggtagcgatt gggtgctccg
     4621 agggcctgag aagaagcgaa caatttatcg ctcttttgtg acgtgcattt cacgacagtt
     4681 tttgcccaaa tgatatcagc agattggcta aacgacgaca aacaggttac tgaaaacagc
     4741 aacaggcaca aggacctaat gatcataatc cctatttgaa cgtaacttcc acgcgttggt
     4801 tgcgtttacg gccttcaggt gtactatttg tatcgattgg ttgcgatgag ccataaccaa
     4861 ctgtactgat ccttaccggg tctatgcctt tattctgtaa atattcacga acagcgttgg
     4921 cacgatttaa actcaatttt tcacagtaag cttcgctaca taatccattt gtatgtccgc
     4981 cgatctcgat gattaaaccc ggattggcaa tcataaaatc atacaaccta tttaactcct
     5041 cgaaagattc aggttttatg atcgatttat tggcatcgaa atacaagtta tcgagaatga
     5101 ttttttcacc gctgtgtaat ggtgttaatt taaaatcgac acgttttact ttttgtgtgc
     5161 ggaagtcctc gatattaatt gtcatttcaa gcggtaaata accttctctg atcactgtca
     5221 ctttgtattg cacactatct gttactttca atcgatacgt attattcaac gtattcatgt
     5281 cgatagggtc gctctcataa tcagtcagaa cgaaggataa ttttgcattt atcggcacat
     5341 tggtattcgc atcgaaaaca gttccaaaca ataggatttc atttgtcagt gtaatattca
     5401 atgcttcttt ttcaggaact acaacttctt cttcgaaaag aatttccccc ggatctgcag
     5461 gtttatccgg tgtgatgata tcagcaactg ttgaatcacc gctagttaaa actacatcag
     5521 aaagtatttc agctaatact gagtcttcga tttccttgac ttcttttaaa ggaatccgat
     5581 aaatatcagc attgagttta tcatctcccg tagaagagaa atatgcgtaa tcagcagtat
     5641 cgggatatgt aaaataagct tcgaatcctt tagaatttac aagacttcca agattttcag
     5701 gttttgtcca atgtttccat gtactatcca ggcgtacaca aacataaaca tcctgatcgc
     5761 cataacccgg taaaccatct gaagaaaaga ataatgtttt tccgtcagga cctaagaatg
     5821 gcgccatttc gctggaagct gtgttgacat cggttcccag attaataggt tcagtccatt
     5881 caatttgatt atcgctgaag cttacataaa tatctttcat tccatggctg tcggcacgct
     5941 cgagtgacaa caataacaca tcgcgatcgg aattcatgaa atattcagca aaccagccat
     6001 gtgttattac acccggtgtg ataagatttt ttggaaacac ccaacggcca tattcagttt
     6061 tccatgtttg cgcaatacca attaaacttt caccggaagg agaataagta tttgcaagcg
     6121 taagcatttc cccattcatg gatatgccga caacataatt attgttttca ttattcatcg
     6181 gtccaccgat atttgttgct tcagtccatt tggtgccatc ataagtagaa acccaaatat
     6241 catcattcat gatggttcct gtatttccca tgtggttttt cctggtgaag tacatcgttt
     6301 ttccatcagg cgtgagcaag ggagtaatct ccgaaccata agtattaatt gttggtccaa
     6361 ggttttggct tttaccttta aaagtcactt tgtcagtaac attgatagag cctcttggaa
     6421 tttcaattgg tgcttctgaa atacctatag catcaatttg attaaatcca tctatacttt
     6481 cagtatcaag aacaagcttc aaatcattgg atgcaaaatc agtaggtgta atgttgatgg
     6541 aaaacacacg gcccaattcc catttatttc cttcggcggg acgttcccaa accaaatggg
     6601 gaatattata ttggtcgaag agatagatcg cttttattgc acctgcatta aagctttcat
     6661 ttatcacaat ttgtgacacg cgaattcttt tatcaaatcc gactctgata tattcctcaa
     6721 agaagttctc agacatcgag ggcgaccatg cgcaaccgca atcgccgaaa ttcggcaaac
     6781 gagatggagg tcccaatact tgcttggcag agtaggagtt caaatccaat tgcgacgaat
     6841 aggagatgac cttggaggcc cacatgattt cggcagcaga gcatgtataa ccggccagga
     6901 tgaatagaaa taataatgtg tgtagtttcc tcataatgtc acaattcctc aggtttgcgt
     6961 aaaattattt aaacctgacg ggctataaaa ttacaaatac ggttaatttg taacagtgta
     7021 aaagcaatta attgcaaatc tgtatagaaa tttgtccgca tgaccagatc agaaaaggca
     7081 caattcattc tttccaagct aaatgagttg ttccctgaaa ctcctgttcc cttgcagcat
     7141 aaagatgcgt atacgttgct tgtagcagtg gtattatcag ctcaatgcac cgatgagcgg
     7201 gtgaatcaaa taaccccaaa actctttgcc agagcggata atgcctatga tatggttaag
     7261 gttccggttc aagaaatcca cgatatcatc aaaccctgtg gattggcacc aatgaaatcg
     7321 aaagggatac atggcttgtc ggaaatgata gttaccaaac accacggaca agtgccttcc
     7381 acttttgcgg aattagaggc catgcccgga gtaggccata aaactgcaag tgtagtgatg
     7441 tcacaagcat tcgattttcc tgcatttcct gtcgatacac atattcaccg ccttgcgtgg
     7501 cgatgggggt tgacaaacgg caaatccgta gaacaaaccg agagagacct caagcgcatc
     7561 ttcccggaga atacctggaa taagctccac cttcagatca tttacttcgg acggaagtat
     7621 tgccctgccc gaggacatga tagggaaaaa tgtcctattt gctcagttgt aggaaggaaa
     7681 ttgatgtaaa aaaaagggct gcccgattgg acagcccttt gttatgccgg tcgttagttt
     7741 acttagtcaa tacgatttgt ttgcctacaa ctgcgtcatc actgataata ttcagttgat
     7801 atacgcctgc agcaaacttg gtcatatcaa ttacataagt agctgacaat gaattagtca
     7861 tgttagctga ccaaacctct tgaccgaggt aatcagtcaa tttgaattga atatcagccg
     7921 ataccggtaa agaaacttgc acggtagcat agtcgcttgt agggttaggt gtgatttcaa
     7981 ctgcttcagc cagtgtgata tcgttcactc cgtttccaac ttcaacaacc atactgtagg
     8041 tatccgaacc acaagggtta gttacggtca atgaaaccgt atacatacct gttacagcat
     8101 aagtatgtga agggttgatt gttgttgaag ttccggcatc accgaagctc cagctataag
     8161 aagcaccatc ggtagatgtg ttggtgaatg ttacggtgag tccgcttgca ctataggtga
     8221 agctggcttc aggagtataa ttcactgtta cggtaacttc atcagtcact gaacaacctg
     8281 ttgtagggtt ggtaactgtt acagagtagg tgcccgattc tgttacatcg atagtttgtg
     8341 tgctttcgcc ggtgctccag ctatagatgc tgccaacgtt acctgcatca agcgttacca
     8401 ggtcgcaaac cgtcatatta ggaccaaggt caactgatgg tgtagcgagt gaagtgatag
     8461 taacacagat catgtcatta ccttcatcag agtcgcctgc aaaatcagtc caaacgcaga
     8521 ggtcatatgt tcccggaact gagaaatcgt aagatacatc gaatgtatat gttgcagtac
     8581 ctcccggagt gatggtaact gttgcgaaat ctgattcaat agcgccacca tcaacttgat
     8641 atttgattgg gaaggtagat actgttgaag gaccgaagtt ttctacagta acggtaatgg
     8701 tttcaacgcc catgccgcaa ccgccttcag gtgagatgat ctcaactaca cctaagtcaa
     8761 ccggagcgca gagcttgatg gtttcataga tattctgaag caggttttgt gcatcagggc
     8821 taggagagtg gaaatacgga ggtgtcatgc cgccgaagat tacatgtcca tcgccccatt
     8881 ctttttcagc cagcacataa cgtgaagcat cataagtatc gataatcaca ggatcaatgc
     8941 ctccacctga tacagaggca tggccgaagc ttccgccggt ataatcagtg gtgattggtg
     9001 tccaaggacc gttgaagatt ggatgtgatg gatcgtaagc ggttacgttt gatgtatacc
     9061 aagagtagtt caaggtaacg ccatcaaatc cgaaatccat accatcgcct tcatttggcg
     9121 ctgagttaag gaggaggtga cctcctgatg caacccagtt ttctacgatg ctgctattgg
     9181 ttgtcaggaa gttttccagt tcactggctt gagcatcaga gccttcgagg taaacgaagc
     9241 aagtgttctc atcgaatacg gcataaggat cgagtgtttc cacgtattcg aggaaccaac
     9301 cgtcatcgcc aaacactgca tccatggcat ctgagttaga ggtaacatac caaggttcgt
     9361 agcctccgta aacattagaa taaatataga aggcatctgt gccgctaacc ggtgtaaggt
     9421 tgacaacgat agcattcaag gtatcgtttg tcagatcttc atcggaagca agttcagtcc
     9481 aggcggttac attgtaaaga ccatcagcag agaaatcttc agtagctgcg aaggtcatta
     9541 cggctgttga acccggatca actgtgcctg taaataattc agtatgcact gtaccggctc
     9601 caactttata ggctacgtta aagcctgttt gaggtaatgt tccgaagttt tccaataaca
     9661 cgcttacaac ctcagaaccg gttaaggtaa cagcagattc aggagaaata aggtcaacaa
     9721 caccaacatc gttcgggaat ggatcctgaa ttttcacgtt gtcgaatgca aagccgtcat
     9781 tatagttcca atagctatcg gatgcgaaaa tgattctgaa acgcacgcta ggctgaccgg
     9841 cgaggaatga catgtcataa tgcgctgttc tccagccggt ataagtactt tgccaaccac
     9901 gataatctga aacgtagaaa gtaggccaca ttgaatagca atagtagcta tcataccagt
     9961 tttcaccttc acctacgttt ccgaggtcgc tccatgaagc accttcatca agtgtatatt
    10021 ggattttggc accatcggaa tataactgaa tgtcgtagtt gatatccaat tccatgtatg
    10081 gcattaccag agaggtaaag tccatacaag gagaggtcac atatcctttt tcgttattgt
    10141 cataataacc ttcaagattg gtaacccagc tattgagtga gcctggagtt gccgtaggcg
    10201 gagtgttgat cacataacct gcaggagttc caagttcgaa ggtagatgca cctccaacaa
    10261 gccatccttt gctaccatct tcgaagtctt cgtagtaagg gaatgatgcc actgttggta
    10321 cgttgtatac tgttgaataa gtggtatcgt taccataaat ggtatctgtt gcaagatctg
    10381 tccatgtaac gatattgtaa tcgcccggaa ctgccatgtt tacggtagtt gcgaatgagt
    10441 aagtaacaat ttcgccaaca tcaattgtac cgggaatagt ctcggtcaca atagtaccgc
    10501 catcaacctg atagcttaca ggcacatcag tttgagagag gaaacctacg tttttcacat
    10561 tgattgtgat ggtttccgca cctgtcaaac cacaagtaga ttcaggtgaa agtatagctg
    10621 ttgttgccat atcattatct gaaatcgtac aggttgcaag gtaagagatt gcatttctgc
    10681 ggaaattatc tgcatcagga agcggtgagt ggtagttagt aactgtcata ccaccaaaca
    10741 gcacggttcc tgctccccat gatttttcag ccaatacaat tttgcttggg ttgtaataat
    10801 cataaagaat tacagaaagg tcatcaccac taactgttgc atggccataa gaagaaccat
    10861 acataacagt tgatgtaggt gtgaaaggac ctgtaaatac agggtgagtt ggaacgtcag
    10921 ttcctactgt atttgagaaa tatgggtagc taagttcaac accatcaaaa tagaaatcca
    10981 tgcctgaacc ttcatttggt gcagcgttga gcaggaggtg tccaccggaa gcaacccagt
    11041 tttcaattgc agtatggtta gcatcgagga atgatttgag ttccagtgca tgtccatcag
    11101 aaccttcgag gaacacaaaa caagttcctg tgccgaaaac cactgcaggg tcaacggttt
    11161 cgaagtagcc catggtccat tcaccaacac cgaaaacggc atccatggat tgtgagttgc
    11221 tcacagtata ccaaggttca ccgcctgtgg tgttagaaat gatataatag gcattatcac
    11281 cggtaagtgg ctcgaggttg gagatcactt cggtgattga atcgttagac atgtcttcat
    11341 cggtggctaa tgaagtccag gcttcgaagg tataatctcc gagaacgctg aggtcttcag
    11401 tagttgcgaa atcatagtta acagtttcac ccgggtttac agttcctgtg aaagtttctg
    11461 tatgaacagg gccgccttca aagcgataag aaacagggaa tcctgactga gcaagtgttc
    11521 catagttagt aacagctact gaaacagctt cggtagctga cagcagaggt ccagaaacag
    11581 gtgctgtgat gttagaaact ccaacatcgt tagggaagag gtctgatatt tttacgttat
    11641 caaatgcaaa accatcataa tagctccaat aaagatcggc gcccataacc atgcgcaatt
    11701 tcactgaggg ctgaccgccg aggaaagcaa gatcctggta gccatgttgc catccaccgc
    11761 tattgccatc ccagccgcgg taatcggtaa catagaaatc aggccacata ccatagcagt
    11821 tatagctggt ataccagttt tcaccggtac ctacatcgcc aagatcgctc caagtggcgc
    11881 ctgcatcagt tgaatactgg attttggcac catcggaata catttgagta tcccagttca
    11941 ggtcaagttc aaggtaagga aaaacaagtg atgaaaaatc gaaacatggt gattcgatat
    12001 atgatttttc gccatcatta tagtatcctg taaggcttgt tgtccagcta ttcacactac
    12061 ccggagtagc aggagggggg ccaacgattg cgaaacctgc agggcttccg agttcccatg
    12121 agctagcgcc tccaccggcg atccatccac cgtcgccggc ttcaaaatcc tgatagtatg
    12181 gaaatacgtc aacaacagga atattattaa tggtaaccgt aattgtatta tttgcaggta
    12241 tttcatctgt tgccaggtcg caggagatca cgaaagtatg tgctcctaag gcagtaatat
    12301 ctgcgggaac ggcaaaacta taggttacag tttcaccggg agcaaccgga ccggcaattg
    12361 tagctgaaac cactgcgccg ccatcaagct gataactaac cggaatattt acttcatcat
    12421 tcgtgccata atttttatag ttgacactca cggtttccac gcccatgccg catcctgaag
    12481 atggactggc aagagaaaga gaggcaatat catgatcagg tatctcatag aatgcgagat
    12541 agttgaggat attttgaaag aggttgatag attctaatga tggataatgg aagttatcgg
    12601 tagtcattcc gccaaagaac acaataccat cagtaccata gcgaccttca gaaagcgcaa
    12661 ttgtagttgg tgtaaagtcg tcgatgatca gcggtgtaag atccggaccg gaaacgctgg
    12721 catggctgaa ggaagaacct gtccatgacg ttccggtagg ggtgtacggt ccgttgaaaa
    12781 tcgggtgggt agggtcggta gcatgcacgt tgctcgaacc tccccagtag gtgaggttta
    12841 cccctccaaa tccgaacgac ataccgtcgc cctcatttgg tgcagcgttg agaagtagtc
    12901 gtccaccacc ttcaacccaa gtttcaatga catcaatatt cacagagagg aatgactcca
    12961 gctcatcggc gaagccatcg cttccttcca ggaatacgaa gtttgtgttt gcagaaaata
    13021 ccaatgccgg atcgcaggtt tcgaagtaat cttgtaacca gccgccaaca ccgaatactg
    13081 cgttcatagc atcggaattg gaagtagtgt accagggttc gtaaccaccg taggcattcg
    13141 agtagatgta gtagttgtta tcaccaccgg cgatagcatc acgcgtcgga gccataaagg
    13201 cgagcgttag catacaactg aggagaagcg taaggtgttt tttcataaaa gtctgttttg
    13261 gctgacaatc tttgcggact aagatacgcc ctagaaaagt gtacttcaaa gaaaaaagcg
    13321 taaaaaaatg tataataagt tagacgagcc cttatttatc aacaatttaa caaaaagtac
    13381 acaaaaaagg ccgaagtaaa actccggcct ttgatgaagg tttccctttt tatttggtca
    13441 atatgatttg tttagtggca gttccattat cgcctgtaac tttcaggtta tagactccgg
    13501 cagccatatt cgtcatatca atattgatac tttcagtcaa aatgttacca ggattatttg
    13561 tccaaacagt ctgaccgatt tggttcaaca gttccaaggt aatattctgc gcttcgctga
    13621 gagcaatatt tacattggtg cgaccatctg tcgggtttgg atataccgta acactttgat
    13681 caagttccat atcagttatg gcagttccaa cttcaataac aacgctatag aaatctgaac
    13741 cacaaccgtt agttacagta agggtaacgg tatacatacc tgcagtagca taagcatgag
    13801 atggatttgc agtagtgctg cttccgccat caccaaaact ccagttataa ctggcaccat
    13861 cggtagattc gttggtaaat gttacagttc caccggtaac agtatacgag aagtttgcaa
    13921 caggtgtata gtttacagta actgttatgt tatctgtaat tgaacatcca cttgtcgggt
    13981 ttgtaacagt tacggaatat gtacctgaag tagtaactgt gattgtttgt gttgtttcac
    14041 cggtgctcca caggtatgtg cttccaacgt ttaatgcatt taataccaca tcgtcgcaca
    14101 cagttgtatt tggtccgaga tccagaaccg gtgttgcgag tgaaacgatt gatgatatta
    14161 atgtatcgtt tgtttcatct tcatcaccac tgaaatctgt ccatgtagca agtgtatgcg
    14221 tacccggagt tgctagatta gctgttgcag taaatgtata cgtataagta ccaccgatag
    14281 gcacagggcc atcagcaact tcagtaacaa ttgctccgcc atcaacctgg aatttcattg
    14341 gaatatttgt aacggttgaa ggaccgaagt ttgatactat aacgctaact gtttctgttg
    14401 cagtagttcc gcaaccaccg tcaggagaaa gcaatgtagt aacacctaaa tctacaggtg
    14461 cacaaagctt cagatagtca ataatattct gacgtaaatt ctgattatca ggagaaggag
    14521 aataatattc cggagcactc atatttccaa agaacacgcg accatcgccc caatcttttt
    14581 gacttaggat atagcaatca tcacaataaa aatcgttcat tattggtgtt acatcgccgg
    14641 tagtgcgtgc ataagcaaca tagaatcctg taaagtcaga agtggtagga gtaaatggtc
    14701 cgaaataaat cggatgtgtt ggatccgttg cagctgtttg ataagtatag tatgggtaaa
    14761 caatacttac accaccaaat ccaacatcac caccatcacc ttcccaagga gatgcattga
    14821 taaataaatt acctccagat gctacccatc cttgaataag atcactattg tcttcgaaga
    14881 atgattcaaa ttcactccaa tgaccttcac tggcttccaa gaaaataaag caattgcttt
    14941 cactgaaaac ggtatatgga tctatatctt cataataatc cagtgtccag gcatcatcgc
    15001 caaacacttc atccattgct gttgaatttg aattcatcca tccgggttca aaaccaactg
    15061 tgctgctata gatataataa gcaccggtac ctgaaacagg agccaggtgc attacatcgg
    15121 cacatcccgg aatttcatca tttgttacat catcatcagt agcgagcgat gtccatgcgc
    15181 aaatttcgtg taatccttca gcacttaaat cagcagttgc tgcgaatgta aatgttgcac
    15241 ttgcaccggc agttaatgtt cccgtaaatg tttctgtatg aatagtaccg gcatcagctt
    15301 ggaaattaac agggaaacct gattgaggta atgttccaaa gtttttcaca gtgacactta
    15361 ccgtttcagt tgttgttaaa tcaactccgc taacaggagt aatcaaatca gtaacaccaa
    15421 catcatttgg ccaaggatct tgcactttga aattatctaa tcctatacca tcgaaattaa
    15481 ttattccgtc agcagagaat ttaaatctga atttaacatc agtttcaccg gcaaggaaag
    15541 aaatatcatg atgtgaagta acccatccac cgccgctgcc tacccagccg gtttcgtaat
    15601 cgagggcaaa ggcagtattg gtataccagt tatcgcctgt gccgatatca cctaatacag
    15661 accatgtaac accaccatta gtagaatatt ccaattgcat tccatcccaa gtgtcttcag
    15721 tttcccacca aagatctact tcaacatatg gaattactaa agatgaaaga tcaaaacatg
    15781 gtccttcgag ataactattt tcattgtcat tataattgcc cgtaatgctt gtcaaccatg
    15841 aatattgact tccgggagta gcagtaggag gttcattaat tacaggaccg tcgggatatc
    15901 ccaattccca tgtagatgaa gcgccatatg ccatccaacc atcttcatct cctaattccc
    15961 aattttcata atatggatat gtagttatta ccggaatatt agcaagtgta gaataagtag
    16021 tgtcgttatc attaattgta tcagcaggta atcctgtcca tgcattaagg gtatgatcac
    16081 cgatgagtga tatatcgacc ggagtagcga aagtatataa aactacatca ccaactccaa
    16141 ttgttcccgg cacgacctcg ctcacgatag ttccaccatc tgcttgataa tacacaggaa
    16201 catcagtttg cggcaggaag ccataatttt ttactttgat tgtaacaatc tcttcatctg
    16261 tcatgccaca acctgtttca ggtgattcaa tgctatacac accaacatca tcatttgaaa
    16321 tggtacatgt ggcgaggtaa gcgatcaaat tctttctaaa gtttcctgct tcagtcagcg
    16381 gtgtatgaaa atctgtaact gtcatgctac caaaaatcac agtacctgcg ccataatttc
    16441 tttctgcaca aataatgcgt gatggtgttg cagcatcaaa taatatgggt gataaatagg
    16501 cacccgaaat acttgcatgt gcgtaattga aaccggtcat atcggttgag gtaggaacaa
    16561 atggtccgtc ccaaaccgga tgagtaggat cacttgctga aacggttgat gcataccaag
    16621 gataatcaag atgtacgcca tcaaaaccga aatccattcc atcgccaaca ttcggtgctg
    16681 cattgattaa taaatgtcca ccggctgcaa cccataattg catggcatcc atatttgcat
    16741 tgatgaaagt ttccattgca tcagcgtgcg aatcagaacc ctccataaaa atgaagcaat
    16801 tgtcgaggct aaataaaact gccggatcaa ttgtttcata atattcagta gtccattcac
    16861 ccacaccaaa cacatcatcc atatgttgtg aatttgatgt ggtgtaccat ggttcgaaac
    16921 ctgttgaatt actatagata taatatgcgt tggtgcctgt gattggttcg atattgctga
    16981 tcgtttctga ccatgtatca ttagtaacat cttcatctga ggcgagacct gtccatgcaa
    17041 caaaatcata atcaccatcg gcacttaaat cgcttgtagc tgaaaatgtg aatgtagcaa
    17101 cttcagcaac accaagtgtt ccggtgaatg tttcggtatg aatagtacca ccatcaacct
    17161 gataactaac agggaaacct gtttgaggat ttgttccaaa atttgtcact tcaacggtta
    17221 ctgattcagt agctgaaagg aaaggacttg tttcaggaga aataacatta gttacaccaa
    17281 tatcgttagg cagcgggtcg ccgatccaca tattatcgaa tgcgaaaccg tcgttgaaat
    17341 tccaataaga gcttgaggcg aaaacaaatc tgaatttaac agatggttca cctgccagga
    17401 agctgaggtc atgttttgaa tgcacccagc caccactgtt tcccgaccag ccgttatagc
    17461 tatcgatgta gaacgttggc cacatggcat agcaggtatt ggtattatac caattgtcac
    17521 ctgtaccgat tccgccaagg tcattccatg tcaacccttc gtcagtagta tattgcagct
    17581 tggcgccatc atcatataac tgaatatccc agttggcgtc aaattccata taaggtatga
    17641 tgagtgaact aaagtccaaa cacggtgaga tcacatacga tttttcattg aggttataat
    17701 aatcggtcag ttctgtggtc caactgttga gacttgtagg agtagcagga ggggggccga
    17761 tgatggcaaa tcctgccggg tcgcctaatt cccaggtgct ttctgttcca cctgaaatcc
    17821 atccgccggc accatcctca tagtcttggt aataagggaa ggtggttact attggaatat
    17881 tggtaaacgt ttcatctgaa gtatcatcag ctgctaccat atcaacaccg taaatagtat
    17941 aggcttgaag cgtatactct ccgagattac tgaaatcggc aggtgtggtg aatgtaaagg
    18001 cgacagaccc caaagaaggg atagcagcac ctgtataggc ctgaacaacc ggtgccaaag
    18061 ctccgagtgt tggatcatct acctgataag ccacgctgaa tgctccggaa ggttctgtgc
    18121 caaggttagt gacagttaca gtaattgggg ttactcccaa tccgcagccg gttcccagcc
    18181 ctgagataga ggtgacgcca agatcatacg ggccggcggc cgggatattg ataccataat
    18241 cctctacttc gccgaaggta tatgtttcta caactccaca cggatcaatt gcaagggcag
    18301 gaaaatacac acacataacg cgcatacgtg ttgttcccgg aagagcggtg aaaggcactg
    18361 t
//
LOCUS       DAOQHY010000053        18125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1432_length_18125_cov_5.288268, whole genome shotgun sequence.
ACCESSION   DAOQHY010000053 DAOQHY010000000
VERSION     DAOQHY010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 18125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18125
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1432_length_18125_cov_5.288268"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1341)
                     /gene="dnaB"
                     /locus_tag="PLL28_06080"
     CDS             complement(<1..1341)
                     /gene="dnaB"
                     /locus_tag="PLL28_06080"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNF68926.1"
                     /translation="MAESDNSTSNFQKRNERRRPQADLSSVVYGKVPPQAGDLEEAVL
                     GAFMIDRDAVTNAIEILRADSFYDDRHRLIFTAIRRLFEKTQPIDLLTVTEELRRMGQ
                     LEEAGGAFYITSLTNRVASAAHIEYHARIVAQKFIQRELIRISSEIVHDAYEDTTDVF
                     ELLDKAEQNLFNIADKNLRTSFSDINNLLSMTIKEIEEARKHEDMLTGCPSGFSALDR
                     VTGGWQKSDLIIVAARPAMGKTAFTLSLARNAAVEFKKGVAVFSLEMSSQQIVKRLIS
                     AETEIPAEKIIKGNLAEHEMVQLVKLSGRLSEAPIFIDDTPAINIFELRAKCRRLKMQ
                     HDIQLVVIDYLQLMTAGDSSKGGNREQEISQISRSLKSIAKELNIPIIALSQLSRKVE
                     DRGGTKRPMLSDLRESGAIEQDADLVCFLYRPEYYGLDVDENGNPTKGIAEVIIA"
     gene            complement(1544..2125)
                     /locus_tag="PLL28_06085"
     CDS             complement(1544..2125)
                     /locus_tag="PLL28_06085"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HNF68927.1"
                     /translation="MRQIIVIALLLLTLSSRAQVMVDIPTMMQEEQLARDVYQLANEK
                     YQMHVFECKINAETNHWTMLRSYSETPNLEMPAYGSLSDSIFKAAFSDYQTMVTTSAT
                     EALKAGAMIEETDIFVLEVNRIKAEDEQLKALHDNLITASEQHLSSFLTALKADGVTY
                     TPSLLSEERIQRLMNEGNCKPGAACKGNSSCSH"
     gene            complement(2227..3192)
                     /locus_tag="PLL28_06090"
     CDS             complement(2227..3192)
                     /locus_tag="PLL28_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNF68928.1"
                     /translation="MHPFLAKLLADKAKKEKKSEVLSNYILAREFAKFRLRVRRIVAA
                     VALLAFGVSAAAFGLLGFLLPNHFVDGGAVGVAMLSAQVSGLDFSVLLVVISIPFIIL
                     GYRTIGLQFAIRASIGIVALALAVEFIDIPIITEDKLLVAVFGGFFLGLGTGMTMRAG
                     GVIDGTEIMALQLSKKLGVTVGDVILIINIIIFSIAAWLLSLEIALYSTLTYLAAGRT
                     VDFILEGIEEYTGVTIISPKSEDIRLMITEKLRRGVTIYKGKRGFGSHGHRQAEIDIV
                     YTVITRLEINRLSAEIEQIDPEAFIVMQSVKDTRGGMIKKRAHKH"
     gene            complement(3293..4471)
                     /locus_tag="PLL28_06095"
     CDS             complement(3293..4471)
                     /locus_tag="PLL28_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNF68929.1"
                     /translation="MPNDLFEGVDYYQLDDLLTTEHKLIRDTVREYVKKEVSPIIEEY
                     AQRAETPKHLIKGLAEIGAFGPTIPEEYGGGGLDNISYGLIMQEIERGDSGLRSLVSV
                     QGSLVMYPIYTFGSEEQRRKYLPKLGSGEMIGCFGLTEPDHGSNPGGMITNIKDMGDH
                     YLLNGAKMWISNAPFADIAVVWAKDETGKIRGLVVERGMEGFTTPETHGKWSLRASAT
                     GELVFDNVRVPKENIFPEIKGLKGPLMCLSTARYGISWGVIGAAMDCYDTALRYSKQR
                     VQFGKPIGGFQLTQKKLAEMVTEITKAQLLAWRLGVLKNEGKATPEQISMAKRNNVKM
                     AIDIAREARQILGGMGITGEYSIMRHMMNLESVITYEGTHDIHLLITGAHVTGLDAFK
                     "
     gene            4561..4908
                     /gene="folB"
                     /locus_tag="PLL28_06100"
     CDS             4561..4908
                     /gene="folB"
                     /locus_tag="PLL28_06100"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNF68930.1"
                     /translation="MASIKLEGMRFRSFIGVYADEQAHGNDFLVDITITSEKITSTHD
                     QLEDTLDYVRVYRIAEGVMAERYLLLETAVQEIIKRIRELNFDINVVRVRVAKLNPPV
                     GGDMHSVSVEMEG"
     gene            4962..5032
                     /locus_tag="PLL28_06105"
     tRNA            4962..5032
                     /locus_tag="PLL28_06105"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4994..4996,aa:Cys,seq:gca)
     gene            complement(5158..6618)
                     /locus_tag="PLL28_06110"
     CDS             complement(5158..6618)
                     /locus_tag="PLL28_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68931.1"
                     /translation="MKKMTGFLLGFLVWSLGVFAQTSGGLFSYGPDMNSTRIFPYVSQ
                     KPDGDVMVFGGRSNGFISSAYADLYSIETNTFTNLNMLYTHDNTVMVKLNDGRVLLAG
                     GGFDYGVPAHQYAEIYNPNDNSFTAAGTMAYARMQGSGALLTDGKVLIVGGWYSPEAA
                     ANAELYDPVLMTSSYAGGLLNPRSNALVLPCNDGGALVIGGYPTYGGTTFNAVEYYNR
                     ETSSFELFSDEIISGETGWTTTLDPFYRKTTDMYILENGKYLLMLYRSVPTLEYTLAL
                     FDPENKSFEKVNTDYPLMGAYSDGGIIDYALNKEESLVYMLGVDNESGSTGYCIVTTD
                     LSTGKTYQPDESYTMPSGEYLIFTTLSYIPETGMLLAPGVSTSNTDYFNATAKSFLIE
                     PELKVDIPIQEDLIVLQVYPNPVKDMLMLILPSNIGGQAIVDICDMRGQILYRTNAFG
                     GEKLVIPVNYLPTGNYFLRVRNGNCNLSASFIRAGN"
     gene            complement(6745..7278)
                     /locus_tag="PLL28_06115"
     CDS             complement(6745..7278)
                     /locus_tag="PLL28_06115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HNF68932.1"
                     /translation="MKADSIFEFKDSRDGKTYSAFTINGVAWMSENLRYNSKGSWCYE
                     SDGKSCKEFGRLYNWNDAILVCPNGWHLPNRAEWDSLIVWTGGELKAGHNLAYNDSLP
                     FNIVFGYPPNVNGRYSGGGIQASFWSADEYNSSTAWVYYFLKEKLPLTFSNYFSKNYG
                     MMCRCVKTIADSSSETN"
     gene            7441..8124
                     /gene="mqnB"
                     /locus_tag="PLL28_06120"
     CDS             7441..8124
                     /gene="mqnB"
                     /locus_tag="PLL28_06120"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HNF68933.1"
                     /translation="MSILLTAATQFELKPLIDKGSTTSTGSNSFKLVVPGFPDIEILI
                     AGVGTAATVFHLTEMLMKKKFDLAIQVGICGAFDPSLSKGEVVLVQSDCFAQLGAEDG
                     DDFIDVFDLGLTAHNEKPFVDGKLWCTDKLSGNFQTLRKVSGSTSETGGGNEQNIEKI
                     RNRLHPQTESMEGAGFYYVCGKLNAPAIQIRSVSNYVERRNKANWNIPLAVEQLNTFL
                     IQSLPSIHT"
     gene            8121..8954
                     /locus_tag="PLL28_06125"
     CDS             8121..8954
                     /locus_tag="PLL28_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HNF68934.1"
                     /translation="MKLSLAYSPCPNDTFMFDAIAHGRIDTEGLTFDIHLADVESLNQ
                     QALEEKHHVSKLSFHAFAYVSHQYKLLTSGAALGRGCGPLLISKDEIPKSKIEFCLIG
                     IPGRLTTANFLCTLAFPEAATKKEMVFSDIEQSLLMGGIDIGLIIHENRFTYQQKGLR
                     KIVDLGEWWESKYHMPIPLGGIAVSRELPIEIQEKVNRVIQRSVAYALAHPEMSKDYV
                     CAHAQEMSPEVMQQHIALYVNDFSVDLGVEGKSAVEQLYHVALEKKIIGSLRYPLFVG
                     E"
     gene            complement(8930..9541)
                     /locus_tag="PLL28_06130"
     CDS             complement(8930..9541)
                     /locus_tag="PLL28_06130"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNF68935.1"
                     /translation="MTHDGYIKYEQHWEIAKPLTDIEPLVLMRNQLAALQWIGVYPDG
                     VGYGNISLRLEGADQFLISGTQTGNMLIADASHFCMVEKADINKNILWCKGPIKASSE
                     ALTHAAFYQSDRNICAVIHIHDAAAWKRLLFVNPTVSKEIEYGTPEMAFAMQALIHSQ
                     QNKLCGVIVTAGHADGIFAYGDSLNSAFSVLESAMHHSPTNNG"
     gene            9638..9940
                     /locus_tag="PLL28_06135"
     CDS             9638..9940
                     /locus_tag="PLL28_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68936.1"
                     /translation="MTRYLIGLIIATALFSSCETDPPANALQLDTGYLRNYTGLDGCG
                     WVIINADNQAMEPINLGDFGLELVDYAPISFTYTEVNSVSICMVGPIIELVTVKME"
     gene            complement(9941..11053)
                     /gene="mtnA"
                     /locus_tag="PLL28_06140"
     CDS             complement(9941..11053)
                     /gene="mtnA"
                     /locus_tag="PLL28_06140"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNF68937.1"
                     /translation="MLIKGTQFRTIWLHPDDENCVQLIDQRWLPHEVRIADIRTVDEM
                     CTAIADMWVRGAPLIGAAAAWGMYLAACEVPASCARDFFFEEAYGKLLKTRPTAVNLK
                     WALDEMLTAITGNDSIIESKWYARNRAKWIADDDVKVNTAIGEYGLSLLSAIAEKKSG
                     EPVQILTHCNAGWLATVDRGTITAPIYLAAEKNIPIHVYVSETRPRNQGAQLTAWELG
                     ENNIPHTLIADNAAGYLMQQGKIDIVLVGTDRTSARGDVINKIGTYLKALAAHEHQVP
                     FFVAAPSSSIDFTMKDAFTEAKIEERNADEVTYVQGRADGKIISVRISPESTTAINPA
                     FDITPAKYVTGLITEKGICSASFEGLKKLFPEKFSF"
     gene            11056..12012
                     /locus_tag="PLL28_06145"
     CDS             11056..12012
                     /locus_tag="PLL28_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68938.1"
                     /translation="MRNIISGICTILICFNSISFAQSTDIYLFIDSNTIIGKISDNKL
                     FTGPSEVAYTLSGNTVYVGDSTSKEQILFLVNAKDMLHRKSGVILQADGSTVQYIAMQ
                     NSFYLGDHPIDKDNEELLHFEVINDSLTEVYSGISDLLIGVIEGKLTSQSQVTAVAHQ
                     YIRHFSLDDSVRARMAQFSVDIVTDGKSFIHPKLDMGPYYEWVWDGQTLKPLWGYRPE
                     DEWVFDGKYLKPAWSADPQTEWVWDGKVLKPFWESGVDKQWLWDDAMLKPYWDSNPDL
                     QWTFDEGVWRPMWRFNDAEEWVVEGYVPVPVAALVVLGMADR"
     gene            12079..12585
                     /locus_tag="PLL28_06150"
     CDS             12079..12585
                     /locus_tag="PLL28_06150"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNF68939.1"
                     /translation="MKQIKWMLVVAVMAYSSIASAQQVKIAYINSQELLSSMPEAKAA
                     NDKLVAYAREFDSLYQDYVQEYQMLVNDIQGNPNLSEVAREAKIQDAVMLEDRITSYE
                     KETQQKLDAKKQELFEPIIKNTTEIIKTVAKENGYTHVIDNSLSVVIMAPEGDDILPL
                     VKKKMNIQ"
     gene            complement(12624..14036)
                     /locus_tag="PLL28_06155"
     CDS             complement(12624..14036)
                     /locus_tag="PLL28_06155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68940.1"
                     /translation="MLRHLLSLAVCLLGFGIINAQTPAIDVHFGDIHSINRGSSSVLD
                     DAGGVWFAGTISNGAEGSDDIAIYRIKQSGEILWWHAYGDARNEYVNTIAAGGDGILY
                     ACGDVRDPETEDLNAFIMALDTAGNVIWNQEYGEAATNEEFYQLAIAPDATILLTGFH
                     SALTGTGNDVLLARYAPDGALLEDTVFGTDRNEVGMSIVALPGGNIFVSGDRQTIDGY
                     YNPIFIKLSSDFNVINDIDLTSPHNSGCKSMHLNFQGDLYISGETASFATPEFDVLFI
                     VVDTLGNLVTQTGIPSAGPDAGYDIVQLPDSTYAVTGFAYNPAKGENDMLLMHIDSSG
                     NEIDRLFFGETGADMGYDIKVDAIGNCRLSGFHTNADTTFFSLVIAGFPAQTETENIA
                     SPERFLSINPNPITESICVQTELEVDRVIVYDNLGRRVGDLPKTSGQCFEWNTLLPKG
                     IYYIQMYTSVGNFTKKIIAG"
     gene            complement(14051..16507)
                     /locus_tag="PLL28_06160"
     CDS             complement(14051..16507)
                     /locus_tag="PLL28_06160"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HNF68941.1"
                     /translation="MRIFVSLFISGLLVTSVNAQTIIKREHSAVPINENIHIDGVLNE
                     ASWNQAPIADNFTQFQFKYNMASAYKTEVRVLYDNTAIYIGATMYDPAPDSIQVGMTQ
                     RDEFATADFFGIILDPYLDGNNALEFFVSAAGVQGDAKAYAGAGNFGEDFNWDAVWAS
                     DVKISEIGWVAEMRIPYSAIRFPNVDVQTWGVNYIREVKRNRDKSSWNNFDPQIDGFV
                     KQCGLLKGIEHIEPPIRLSISPYFSSYYDLYSDKINDISSTAWSFNGGADLKYGLNDA
                     FTLDMTLIPDFGQVQSDNQVLNLTPFETYYVEQRQFFTEGTELFNKGDLFYTRRIGGT
                     PYGYYDVYYGLDTSESVIDNPIQAHLLNAAKVSGRTSKGLGIGVFNAVEGETYATIEN
                     SEGEKRTELTGPRANYNVLVFDQQLKNNSYVSLINTNTLRDGNYQDANVTGMEFLVNN
                     KSNIWGVEGGAAYSSIFDADAEPTTGIHHRIRGGKTGGKRRFILGYDYIGSTYNINDL
                     GYLPINNQLNFSFNTSYNSYEPLWHLNQIHMVFETNYLRLVDPEAFQNFSIYGETYGS
                     FRNYMYAGVFFYTEPVTTYDYFEPRVPGRFYTYPVNYNFGGWLSTDYGKRWAIDINSN
                     YRVFDDEGRYRWNVSMSPRWRATDRILIILDAGRYYWPGDVGFVNLMGDSIIMGRRKN
                     VTMEGVLSVNYAPLVSMNFNMRLRHYWSFAEYSDFYNLGENGDLLATTYNSFLPDGTS
                     SDDVNFNAFNVDLVYTWYFAPGSELNVVWKNAIYQFGTLLPENYVDDLNQVFDSPTNN
                     NFSIKVLYYLDYLYLQKRKA"
     gene            complement(16620..17579)
                     /locus_tag="PLL28_06165"
     CDS             complement(16620..17579)
                     /locus_tag="PLL28_06165"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF68942.1"
                     /translation="MNKIALSLAVISLLLGACKSKLEKKDQSLDEMQEDAGKQENYVP
                     VLESADATPVYKKIGDSVEMHLNIYYPKKHDKQAATPVIIYFFGGAFIHGSPGQYEDH
                     CKYFASRGIVAITADYRVISRNKGNAINCVVDAKSAVRYVREHAKELNIDPNKVIVSG
                     GSAGAFLALTCNMNDPKWQDSTDNAQVSCAPNAMILLNPVVNSMEHKFRIQKFKDDAK
                     QPDSESHAAEINPLTSIRAGMPPTIIFHGTKDKISGYQFVKQFADDYEKAGNRCELVT
                     YEGQKHGFTAIKFEDGKYYRDALKKSDEFLISLGYLSGKPTVE"
     gene            17903..>18125
                     /locus_tag="PLL28_06170"
     CDS             17903..>18125
                     /locus_tag="PLL28_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68943.1"
                     /translation="MKKHLAQFSLLLLLTVLSSCAKIYYSPDAYSLAQSHQMIAVLPP
                     TVSIAASKKIDAESLKEQQKTESLNFQKEM"
BASE COUNT         5364 a         3913 c         3536 g         5312 t
ORIGIN      
        1 agcaataatc acctctgcaa tacctttggt aggattgcca ttttcatcca catcaagtcc
       61 gtaatattca ggacgataca agaaacatac caaatccgca tcctgctcaa tggcaccgga
      121 ttcgcgcaag tcggacaaca tagggcgctt agtgccgcca cggtcttcaa ctttacgact
      181 taactgtgaa agggcgatga tgggaatatt caattctttg gcaatcgatt tcagtgatcg
      241 tgatatctga ctgatttcct gttcacggtt accacctttt gaagaatctc ctgccgtcat
      301 gagttgtaag taatcgatga caaccaactg aatgtcatgt tgcattttca aacgacggca
      361 ttttgcgcgg agctcgaata tattgatggc aggtgtatcg tcgatgaaaa tcggcgcttc
      421 gctgagtcgc cctgacaatt tcaccaactg caccatttca tgttcggcaa gatttccttt
      481 aataattttc tcggcgggaa tttctgtttc agcggagatc aaacgtttca cgatctgctg
      541 tgaactcatt tccaatgaga aaactgcaac acctttttta aattccaccg ctgcatttct
      601 ggcaagcgat aaagtaaatg cagtcttacc catcgccggg cgagctgcca caatgatcag
      661 atctgatttt tgccatccgc cggtaacacg atcgagcgca ctaaatccgg aagggcatcc
      721 ggtgagcata tcttcatgct tcctggcctc ttcgatctct ttgatggtca tactcaagag
      781 attattgata tcgctaaagc tggtgcgtaa gtttttatct gctatgttga acaggttttg
      841 ctctgcttta tccaataact cgaagacatc agtagtatct tcatatgcat catgcacaat
      901 ttcggatgag atacgaatca actcacgctg aatgaatttc tgagcaacaa tacgtgcatg
      961 atattcaata tgtgcagccg aggcaacgcg gtttgtgagc gaagtgatat agaaagcacc
     1021 acctgcttcc tccaactgcc ccatacgacg cagttcctct gttaccgtaa gcaaatcgat
     1081 gggttgcgtt ttttcaaaca gacgacggat agcagtaaaa atgaggcgat gcctgtcatc
     1141 ataaaagcta tccgcgcgca aaatttcaat agcgttcgta acagcatctc tgtctatcat
     1201 gaatgctccc aataccgctt cctccaggtc gccggcttgc ggaggcactt tgccatacac
     1261 taccgaagaa agatcggcct gtgggcgacg gcgctcattg cgtttctgaa aattagaggt
     1321 gctgttatca gattctgcca tagttcacgt tcgctgagtg gttggtgtcg gtttgttgct
     1381 gtcagcgggg tgccaaaagt agtcacccta ccgcttgtaa cacaacaatt ttcaggaaaa
     1441 ataaatgcac atccaaacgt tatttaacaa cgtatgaaaa gcctgatttc attgtaattt
     1501 acaacaataa aaagaatttt cccaccaatt atccacattg cgattagtgc gaacaggagg
     1561 aattgccttt gcaggcagct cccggcttac aattgccctc gttcatcagt cgctgaatgc
     1621 gttcttccga tagcaggctt ggtgtataag tgacgccatc ggccttcagt gctgtaagaa
     1681 aactgctcag atgctgctct gaagcagtga taaggttgtc gtgcaaagct ttcaactgtt
     1741 cgtcttcggc tttaattcta ttcacttcca gaacgaaaat atcagtttcc tctatcatag
     1801 cacctgcctt taatgcctct gtggcacttg tggtaaccat agtctgataa tctgaaaagg
     1861 cagctttgaa gattgagtca ctcaagctac cgtaagccgg catttccaga tttggagttt
     1921 cagaataact ccgcagcatg gtccaatggt ttgtttcagc atttatcttg cattcgaaca
     1981 catgcatttg gtatttttca ttcgctaatt gataaacatc tctggcgagt tgctcctctt
     2041 gcatcattgt tgggatatca accatgacct gcgcacgact gctcagcgta agcagtagca
     2101 atgcgattac tataatttgt cgcatacatc atcttttaat acaagacaaa ggtattgccg
     2161 ggagaaacgg tgatttgtgc ctgaaattac catgattggt gacaagtatc aatcagtgat
     2221 caggtactaa tgtttgtgcg cccgtttctt gatcatgcca ccgcgggtat ccttcacact
     2281 ttgcattaca ataaatgcct ccggatcaat ttgctcgatt tcagcactta atctgtttat
     2341 ttccaaccga gtaatcacag tatatacaat atcaatttct gcttgtcgat gcccatgcga
     2401 tccgaaacca cgttttcctt tatagatagt aaccccgcga cgtaattttt ctgtgatcat
     2461 caatctgatg tcttccgatt ttggcgaaat aattgtcact cccgtatatt cttctatacc
     2521 ttcgagtata aaatcaaccg tacgtccggc tgcgagatag gtaagagttg aatacaatgc
     2581 tatttcgagt gaaagtaacc aggctgcaat agaaaaaata ataatattaa taatcaggat
     2641 tacatcgcca acagttacgc ccaatttctt gctgagttga agtgccataa tttcagtgcc
     2701 atcgataaca cctcctgccc gcatggtcat tcccgtacct aggccgagaa aaaatccacc
     2761 gaaaacagct acgagcagtt tatcttcggt aataataggt atgtcaataa attcaactgc
     2821 taatgccaat gccacgattc cgatggaggc acggatagca aactgcaatc caatagttcg
     2881 ataacctaat atgataaatg gaatgcttat caccaccaac aatactgaaa aatcaagtcc
     2941 tgaaacctgg gcacttaaca tcgctacgcc aacagctccg ccatctacaa aatgatttgg
     3001 taataaaaat ccgagtaacc cgaaagctgc cgcactcacc ccgaaagcaa gcaatgctac
     3061 tgcagcgacg attcgacgta cgcgcaatct gaatttcgca aattcacgtg caagaatata
     3121 attggataat acttcacttt tcttttcttt cttggctttg tcagcaagca atttcgctaa
     3181 gaatggatgc atatcggtga atatcgacaa aaaagcgcac aaatcataat gataagtgtg
     3241 ctttgcaaga ttttaactaa atgaaatacg caggtgcgaa atattaattg ttttatttga
     3301 atgcatcgag accggtaaca tgtgcgccgg taatgagcag gtgaatatca tgtgttcctt
     3361 catatgtaat tacagactca agattcatca tgtgacgcat aattgaatat tcgccggtga
     3421 tacccattcc tcctaatatt tgtcgtgctt cacgcgcgat atcgatagcc attttcacat
     3481 tattgcgctt agccatagat atttgttcag gtgtagcttt tccttcattt ttgagcacac
     3541 ctaatcgcca tgcaagtaat tgtgctttag tgatctcggt aaccatttca gccaatttct
     3601 tttgtgtcaa ttgaaatcca ccgataggtt tgccgaactg cacgcgttgt ttgctgtagc
     3661 gcaatgctgt atcatagcaa tccatagccg caccaattac accccagctg atgccatagc
     3721 gggcagtaga caaacacatc aatggaccct tcaatccttt aatttcaggg aaaatatttt
     3781 cttttggtac acgcacgtta tcgaacacca attcgcctgt agcagatgca cgcaaactcc
     3841 attttccgtg agtttccggc gttgtaaatc cttccatgcc tcgttcaacc acaagtcctc
     3901 tgatcttacc tgtttcatcc ttagcccaaa caacggcgat atcagcaaac ggcgcatttg
     3961 aaatccacat tttagcgcca ttgagcagat aatggtcacc catatccttg atattggtga
     4021 tcatgccgcc ggggttagat ccatgatccg gctctgtaag tccaaaacat cctatcattt
     4081 caccgctacc caatttaggg aggtatttgc gcctttgctc ttcagatccg aaggtgtaga
     4141 taggatacat caccaggctt ccctgaacgc ttactaagga gcgcagaccg ctgtcgccac
     4201 gctctatctc ttgcatgatc aatccgtagg aaatgttatc gagaccgccg ccgccgtatt
     4261 cctcaggaat tgtgggacca aatgcgccta tctcggcaag gcctttgatc agatgcttag
     4321 gtgtttcggc acgttgagcg tattcttcaa tgatgggtga tacttctttt tttacatatt
     4381 cccgaacagt atcgcgaatg agtttatgtt cggtagtcag caaatcatcg agttggtaat
     4441 aatccactcc ttcaaaaaga tcattgggca taaagtgaaa atttccgcaa agttacggat
     4501 ttggctgcat acacaatatt catgggggct tgaaagattg caaatgacta cttttacagg
     4561 atggcaagca ttaaattgga gggtatgcgg tttcgctcat ttattggcgt ttatgccgat
     4621 gagcaggcgc acgggaatga ttttttagta gatattacca ttaccagtga aaagataacc
     4681 agcacgcatg atcaattgga ggatacactg gattacgtgc gcgtttaccg cattgctgag
     4741 ggtgtgatgg ccgagcgcta tttattgttg gaaacggcag tgcaggaaat tataaagcgc
     4801 atcagggaac tgaattttga tattaatgtt gttcgtgtgc gggttgccaa actcaatcct
     4861 cctgtaggtg gcgacatgca tagtgtttct gttgaaatgg agggttgaaa ggggttcaat
     4921 cattatggaa tctcaatttt ctgtgtaatt ttgccgctca tggtcggatg gccgagtggc
     4981 taggcaaggc tctgcaaaag cttttacagc ggttcgaatc cgcttccgac ctcaaaaaaa
     5041 accggcgttt tcaataacgt cggttttttt gtttgttgac ttctcagtca aatgaagaac
     5101 gcaattctcc tcgctgtgtg cagctagcga ataaaagtgc ctctatacat caaaacatta
     5161 atttcccgcc cgtataaaac ttgccgatag gttacaattt ccatttctta ctcgcaggaa
     5221 ataatttccg gttggcaggt aattaacagg tattactaat ttttctcctc cgaaggcatt
     5281 tgttctgtat aatatttgtc cacgcatatc acagatatcc actattgcct gacctccgat
     5341 atttgaagga agtatgagca tcagcatgtc tttaaccgga tttggatata cttgtaaaac
     5401 aataagatct tcttgtatag gaatatcaac ttttaattca ggctcaataa gaaaactctt
     5461 agctgtagca ttaaaataat cggtatttga agttgaaaca cccggagcaa gtaacattcc
     5521 ggtttcaggg atatatgaaa gtgtggtgaa tattaaatat tcgccggaag gcatggtata
     5581 ggattcatca ggttgataag tttttcctgt tgataaatca gttgtcacta tgcaatatcc
     5641 ggtactgcct gattcattgt ccacacccag catatatacc aatgactctt ctttattcaa
     5701 cgcataatca ataattccac catcgctgta cgcacccatt agtgggtaat ctgtattcac
     5761 tttctcaaaa gatttatttt cagggtcaaa cagtgccaaa gtgtattcca atgttggaac
     5821 acttctatag agcatcaaca aatactttcc attttccaag atatacatat ctgttgtttt
     5881 cctgtagaaa ggatcaagcg tagtagtcca gccggtttct ccggaaataa tttcatcaga
     5941 aaataattcg aatgaacttg tctcccgatt ataatattct acggcattaa atgttgtgcc
     6001 gccataagtt ggataccctc caattacaag cgctccacca tcattgcaag gcaaaaccaa
     6061 tgcattggat cgagggttaa gtaacccacc ggcatatgat gaggtcatta atacagggtc
     6121 atacaactct gcgttggcag cagcctccgg actataccaa ccacctacga tcaacacttt
     6181 gccatcagta agcaatgctc ctgaaccttg cattcgtgca taagccatag ttccagctgc
     6241 agtaaaagaa ttatcattag gattgtaaat ttctgcatac tgatgcgcag gcaccccata
     6301 atcaaaaccg ccacctgcca gcaatacacg accatcattc aatttcacca tgaccgtatt
     6361 atcatgcgta tataacatat taagattagt gaatgtattg gtttctatgc tatataaatc
     6421 ggcatatgca gaacttataa atccgtttga cctgccgcca aaaaccatga catctccatc
     6481 aggcttttgt gaaacgtaag ggaaaatgcg tgtagaattc atgtcaggtc cgtaggaaaa
     6541 caaaccacca cttgtttgag caaatactcc taaactccat accagaaatc cgaggagaaa
     6601 tcctgtcatc tttttcataa ctcatagttt agctaatcaa aagtcaccta aactttgcgt
     6661 aagtagtatg acctctgtca atcacatagt aaacacatat cactattgtg atatctgata
     6721 tttgtcaaca gataaaaaca ataattaatt agtttctgaa gaagaatccg ctatggtttt
     6781 aacgcatcga cacatcatgc catagttttt agagaagtaa ttgctgaagg taagcggcaa
     6841 tttttccttc aaaaaataat acacccatgc agtggaggag ttatattcgt cggccgacca
     6901 gaaactcgcc tgtatgccgc caccggaata tcgtccgttt acattcggcg gatagccaaa
     6961 aacgatattg aagggaagac tgtcgttata tgctaaatta tgaccggctt ttaactctcc
     7021 acctgtccat acgataagag aatcccattc agccctgttt ggcaagtgcc atccgttggg
     7081 acaaacaagt attgcatcat tccaattgta taagcgtcca aattccttac aagatttgcc
     7141 gtcagattcg tagcaccaac tgcctttcga attgtaacgc agattttcgc tcatccaggc
     7201 aacaccattg atggtgaagg ctgaataagt tttgccatca cgactatcct taaattcgaa
     7261 aattgaatcc gctttcatct gcccttgtgc tttaagggaa acaaatgaaa aagatatgca
     7321 gataagtata atgtaatatt tcatggtaat atgtgctaat aacccggcaa agttcgcaat
     7381 ttccattgat ttcagagcct tgaaaaccac gaaaacagga aatcagctta ccttcgcagt
     7441 atgagcatct tgcttacagc agccactcaa tttgagctaa aacctttgat tgacaagggt
     7501 tcaacgactt ccacagggtc aaattccttc aaattggtgg tgccgggatt cccggatatc
     7561 gaaattttaa ttgcaggtgt cggaactgct gctaccgtgt ttcacctgac cgaaatgctt
     7621 atgaagaaaa agttcgacct cgccattcaa gtaggcattt gcggcgcatt tgaccctagt
     7681 ctcagcaaag gagaggtggt gctcgttcag agtgattgct tcgctcagtt gggagctgag
     7741 gatggtgatg acttcattga tgtatttgac cttggactaa cagcacacaa tgaaaaaccc
     7801 tttgttgacg gtaaattgtg gtgcactgat aaactttcag gtaactttca gacactgcgc
     7861 aaagtttcag ggagcacttc agaaaccggg ggtggaaatg agcaaaacat cgagaaaata
     7921 agaaatcgac ttcatccaca aactgaaagc atggaaggag caggatttta ttatgtctgc
     7981 ggaaaattga atgcaccggc tatacagatc agaagtgtct caaattatgt tgaaagacga
     8041 aataaagcga actggaatat tccgcttgct gttgaacaac tcaacacttt tttaatacaa
     8101 tcattgcctt caatacatac atgaaattat cgcttgcata ttccccatgt cccaacgaca
     8161 cctttatgtt cgatgctatt gcacatgggc gaatagacac cgaaggactc acatttgaca
     8221 tccaccttgc tgatgttgaa tcactcaatc aacaagcact ggaagaaaaa catcacgtaa
     8281 gtaaattgag ctttcatgcc tttgcctatg tgagtcacca atataagttg cttacatcag
     8341 gagcagcact aggaagaggt tgcggcccat tattgatttc gaaagatgaa attccaaagt
     8401 caaaaattga attttgtttg ataggaattc cgggtcgact tacaactgcc aacttcctat
     8461 gtacacttgc atttccggaa gcagcaacga agaaggagat ggtattttca gacatagaac
     8521 aatccttgtt gatgggcggc atcgatattg gtttgatcat acatgaaaac agatttacct
     8581 atcaacaaaa aggacttcga aaaatagttg atttagggga atggtgggaa tcaaaatatc
     8641 atatgcctat tccattaggt ggaattgccg taagtcgtga gcttccaata gaaattcagg
     8701 aaaaggttaa tcgcgtcata caaagaagcg ttgcctatgc gttggcacat cccgaaatgt
     8761 cgaaggatta tgtttgtgcc catgcacagg aaatgtcgcc tgaagtgatg caacaacata
     8821 ttgcattata tgtcaacgat ttcagtgtcg acctgggagt tgaaggcaaa tctgctgttg
     8881 aacaactata tcatgttgcg ctggagaaaa aaattattgg cagtttgcgt tatccattat
     8941 ttgttggtga gtgatgcatg gcggattcta ataccgaaaa tgcgctattc agcgaatcac
     9001 cataggcaaa aatgccatct gcatgtccgg cggttacaat cactccacac aatttatttt
     9061 gttgcgaatg aatgagcgct tgcatagcaa aagccatttc cggagttcca tattcaattt
     9121 ctttacttac tgtcggattt acaaacaata atcttttcca tgcggctgca tcatgtatat
     9181 gtataactgc acaaatgttg cgatcgctct gataaaacgc cgcatgtgtc agcgcctctg
     9241 atgaagcttt tatcggtcct ttgcaccaca agatattttt gttgatatca gccttttcaa
     9301 ccatacaaaa atgcgatgca tctgcaatta gcatgtttcc ggtctgcgtt ccgctaatta
     9361 agaattggtc ggcgccctca agtctcaaac tgatatttcc atacccgaca ccatcaggat
     9421 acaccccaat ccattgcaat gcagccaatt gattacgcat caaaaccagc ggttcgatat
     9481 ctgtcaaagg cttagctatt tcccaatgct gctcatattt tatataaccg tcatgcgtca
     9541 tgatctcgtc aaaagtaatg gaatgaagct ttaattcgaa ataaaatact tgtgaaacac
     9601 cctatttgag actaactttg agtatcaaat tttcacaatg acaagatatc tgataggctt
     9661 gattatagcg actgccctat ttagttcctg cgaaacggat ccacctgcaa atgcgttgca
     9721 attggataca ggatatctca gaaactatac cgggttagat ggttgtggat gggtaattat
     9781 caatgccgac aatcaggcaa tggaaccaat aaatctcgga gactttggtt tagaactggt
     9841 ggattatgca cccatatcat ttacttatac tgaagtaaac agtgtgtcta tttgtatggt
     9901 tgggccaata attgagttgg ttactgtaaa aatggaatga tcaaaaacta aatttctcag
     9961 gaaataattt tttgagccct tcaaatgagg cgctacaaat tcctttttca gtgatcaagc
    10021 ctgtgacata tttggcaggt gtaatatcaa atgcaggatt tatggcagtt gtagattcag
    10081 gtgatatgcg cactgaaata attttgccat ctgctcttcc ttgaacatag gtaacttcat
    10141 cggcgttacg ctcttcgatt tttgcttctg taaaagcatc tttcatggta aaatcaatac
    10201 ttgatgaagg tgctgcaaca aagaatggaa cctgatgttc atgcgcggcc aatgctttca
    10261 aatatgtacc tattttattg atcacatcac cgcgtgcaga tgttctgtcg gtaccaacta
    10321 atacgatatc aattttacct tgctgcatta gatacccggc ggcattatct gcaatcaaag
    10381 tatgcgggat attattttca cccaattccc atgctgtcag ttgcgcgccc tgattacgcg
    10441 gacgagtttc acttacatat acgtgaatgg gaatattttt ttccgcagcc aaataaatcg
    10501 gagcagttat cgttcctctg tcaactgttg caagccagcc ggcattacaa tgtgtaagaa
    10561 tttgtaccgg ttcaccggat tttttttctg caatagccga taacaatgat aaaccatatt
    10621 caccaatagc agtgttcact tttacatcat catctgcaat ccatttagcg cgatttcgtg
    10681 cataccattt tgattcgatt attgaatcat taccggtaat cgcagtcagc atttcatcca
    10741 aagcccattt taaatttact gctgtaggcc tcgtttttaa taatttgcca tatgcctctt
    10801 caaaaaagaa atctctggca caactggcgg gcacttcgca tgcagcaaga tacatgcccc
    10861 aggcagcagc ggcacctatc aaaggagcac cacgcaccca catgtcggca atggcagtgc
    10921 acatttcatc aactgtgcga atatcagcaa tacgaacctc atgcggaagc catcgttggt
    10981 cgatcaattg cacacagttc tcatcatccg gatgcagcca tatcgttcga aattgcgtac
    11041 ctttaatcag catttatgcg taacattatt tccggaatat gcactatcct catatgcttc
    11101 aacagcatat cgtttgcaca atcaacggat atttatctgt ttattgattc caatacaata
    11161 atcggaaaaa tttccgacaa taaacttttt accggccctt cggaagtggc gtatacactc
    11221 agtggaaata ctgtttatgt tggtgatagc acttcgaagg aacagatatt atttctggtg
    11281 aatgcaaagg acatgttaca tcgcaaatcc ggtgtcattt tacaagccga tggcagcacg
    11341 gttcaatata tcgccatgca aaatagtttc tacctcggcg atcatcccat cgacaaagac
    11401 aatgaagaac tgttgcattt tgaagtaatt aacgattcgc tcacagaagt atacagtgga
    11461 ataagcgatc tcttgattgg tgtaattgag gggaaattga cttcgcaatc gcaagtcact
    11521 gctgttgcac atcaatatat tcgtcatttt tcgctcgacg acagtgtcag ggcaagaatg
    11581 gcacagtttt cggtagatat tgtcacagac gggaaaagct tcattcatcc taaattggat
    11641 atgggaccct attatgagtg ggtttgggat ggccagacgt taaaaccatt gtggggctat
    11701 cggccggaag atgaatgggt ctttgacgga aagtacctta aacccgcatg gagcgctgat
    11761 ccgcagacgg agtgggtttg ggatggaaag gtgctgaaac cgttttggga atcgggtgtc
    11821 gacaagcaat ggttgtggga tgatgctatg ttgaagcctt actgggacag caatcctgat
    11881 cttcagtgga cctttgatga aggcgtttgg cgaccgatgt ggaggttcaa tgatgcggaa
    11941 gaatgggtgg ttgaagggta tgttcctgtt ccggttgcag ctttagtggt gctaggtatg
    12001 gccgacagat gattgcaggg gcagaaccgt ttctgtatct ttggcgctca atttttataa
    12061 ataaactgtt attatatcat gaaacaaatc aaatggatgc tggtagtagc tgtaatggct
    12121 tatagctcaa tagcttcagc tcagcaggta aaaatcgcat acatcaactc acaggaatta
    12181 ctttcgtcaa tgcctgaggc aaaagcggct aacgacaagc ttgttgcata tgctcgtgaa
    12241 ttcgattctc tatatcagga ttatgtgcag gaatatcaaa tgttggtgaa tgatatacag
    12301 gggaacccaa acctgagtga agttgctcgt gaagcaaaaa tccaggatgc cgtaatgctt
    12361 gaagaccgca ttacttcata cgagaaagaa acacagcaaa agcttgatgc taaaaagcag
    12421 gaactttttg aacctatcat caaaaacact actgaaatca ttaaaactgt tgccaaagaa
    12481 aacggctaca cacatgtgat agataattct ttgtcggtag tcattatggc acctgaaggc
    12541 gatgatattc ttccactggt gaagaaaaaa atgaatattc aataactaac tgaatataga
    12601 ttttgatagc cgggtgatac tgatcatccg gctattattt ttttagtgaa attacctaca
    12661 gatgtataca tctgtatata ataaatgcct tttggcaata aagtattcca ttcaaaacat
    12721 tgccctgatg ttttaggcaa atcccctact cttctcccta agttgtcata aacaattaca
    12781 cggtcaactt ccaactcagt ttgcacacaa atagattcgg ttattggatt tggatttatt
    12841 gacaagaacc tttcgggcga ggctatattt tcagtctccg tttgcgcagg aaaacctgca
    12901 atcaccaatg agaaaaatgt cgtatcagca ttggtatgaa aaccggatag ccggcaatta
    12961 ccaatagcat caactttgat atcatatccc atatctgcac cggtttcacc aaaaaaaaga
    13021 cggtctattt cattcccgga agaatcgata tgcattaata acatatcatt ttcacctttc
    13081 gccggattgt aagcaaatcc tgtaaccgca tatgttgaat caggcagctg cacgatgtcg
    13141 tatcctgcat cggggccagc cgaaggaatg ccggtttgag ttaccagatt tccaagcgta
    13201 tcaaccacaa taaataacac atcgaattca ggtgtggcga aagatgctgt ttctcctgaa
    13261 atatataaat caccctgaaa attaaggtgc atagatttac aaccgctatt atgtggcgaa
    13321 gtcaaatcaa tatcattaat gacattaaag tctgacgata atttaatgaa gatcggatta
    13381 taatatccat caattgtttg cctgtcaccg gagacaaaaa tatttcctcc gggaagcgca
    13441 acaatactca tacccacttc atttctatcg gtgccgaata cagtatcctc caacaaggcg
    13501 ccatcaggag catatctcgc gagcaataca tcatttcctg tgcctgtgag agcggaatga
    13561 aatcctgtta acaaaatcgt tgcgtcaggg gcaatagcta attgatagaa ttcctcattc
    13621 gtggctgctt ctccatattc ctgattccaa attacatttc ctgccgtgtc taaagccatg
    13681 atgaatgcat ttaaatcttc cgtttcagga tcgcggacat caccgcatgc atacaaaatt
    13741 ccatcacctc cggcagcgat cgtatttaca tattcgtttc tggcatcgcc ataagcatgc
    13801 caccaaagaa tttcgccgga ttgtttgata cgataaatgg ctatgtcatc tgatccctct
    13861 gcaccattcg aaatggttcc tgcaaaccaa acgccgcctg catcatccaa tacagaggaa
    13921 gagcctcgat tgattgaatg tatatcgccg aaatgcacat caattgccgg tgtttgggca
    13981 ttaataatac caaaacctaa taagcataca gcaagcgata ataaatgcct aagcatagca
    14041 caaagttagc ttaagctttt cgcttttgca gatagaggta atcgaggtaa tacagcacct
    14101 taattgagaa gttattattg gtaggcgaat cgaacacctg attaaggtca tctacatagt
    14161 tttcaggtaa taatgttcca aattgataaa tagcattttt ccacaccaca tttaattcac
    14221 ttcccggtgc gaaataccag gtatatacca aatcgacatt aaaggcatta aaattgacgt
    14281 catcggaact tgtgccatca ggaagaaatg aattataagt tgtagccaat aaatctccat
    14341 tttcaccgag attgtaaaaa tcactgtatt cggcaaaact ccagtaatgg cgcaagcgca
    14401 tattaaaatt catgctgact aatggtgcgt aatttacact caatacgcct tccatagtga
    14461 cattttttct tcttcccatg ataatactgt cacccattaa attcacaaaa cccacatcac
    14521 cgggccagta atatcgtccc gcatccaata tgattaaaat tctgtctgta gcacgccatc
    14581 tgggcgacat gctcacattc cagcgatatc gaccttcatc atcaaataca cgatagttgc
    14641 tgttgatgtc tatcgcccaa cgcttgccat aatcagtaga aagccatcct ccgaaattat
    14701 aattcacagg ataagtatag aatctaccgg gcacgcgagg ttcaaagtag tcgtatgttg
    14761 tgacaggctc ggtgtaaaag aaaactccgg cgtacatgta atttctaaag cttccatagg
    14821 tctctccgta aatgctgaaa ttttgaaatg cttccggatc taccagacgc agataatttg
    14881 tttcaaacac catatgtatc tgattgagat gccatagcgg ttcgtagcta ttatagcttg
    14941 tattaaatga aaaattcaat tgattgttga taggaagata accgagatca tttatattgt
    15001 atgttgatcc gatataatcg tacccgagaa taaatctgcg cttaccaccg gttttaccgc
    15061 cacggattct atgatgaata cctgtggtag gttctgcatc agcatcaaaa atactgctat
    15121 atgcagcgcc tccttcaaca ccccaaatat tagacttatt atttaccaga aattccatgc
    15181 cggtaacatt ggcatcctga taattgccat cacgcaaggt attggtattg atgaggctta
    15241 cgtaagagtt attttttaat tgctgatcga aaaccaatac attgtaattt gcacgcgggc
    15301 ctgtaagttc agtgcgtttt tcaccttcgc tattttcaat cgttgcgtac gtttcacctt
    15361 caacggcatt aaatacacct attcccaaac ctttgcttgt tctaccggaa acttttgccg
    15421 catttaataa gtgagcttga atgggattat cgattacaga ttccgaggta tcaagtccgt
    15481 agtacacatc ataatatcca tatggagtac ctccaattct tcttgtataa aacagatcgc
    15541 ctttattaaa caactcagtt ccctcggtaa aaaactgtcg ctgttctaca tagtatgttt
    15601 cgaatggtgt gaggttaagc acctggttat cactttgcac ttgaccgaaa tcagggatca
    15661 aggtcatatc aagtgtaaat gcgtcattca atccatactt caaatctgcc ccgccattaa
    15721 acgaccatgc tgttgagctg atgtcattta ttttgtcgct gtacaaatca tagtatgacg
    15781 aaaaataagg tgaaattgac aagcgaattg gaggttcaat atgttcgatg cctttcaaca
    15841 acccgcattg tttcacaaag ccatcaatct gaggatcgaa attattccaa gaactcttat
    15901 cgcgatttcg tttcacttcc cggatataat taacacccca ggtttgtaca tccacatttg
    15961 gaaatcttat cgcagaatat ggaatacgca tttcagcaac ccatcctatt tcagaaatct
    16021 taacatcact ggcccaaacg gcatcccaat taaaatcctc accaaaattt cctgcacctg
    16081 cataagcttt ggcatcgcct tgcacgcctg cggcagaaac aaaaaattca agggcattat
    16141 taccatcaag gtaggggtca agaattatcc caaaaaaatc ggccgtcgcg aattcatcgc
    16201 gctgtgtcat acccacttga atgctatccg gcgcaggatc atacatggtt gcgccgatgt
    16261 aaattgcggt gttatcgtac aatacgcgca cctcagtttt ataggcagag gccatattgt
    16321 atttaaattg aaattgagtg aaattatcgg caattggtgc ctggttccat gatgcctcat
    16381 tcaacacacc atcgatatgt atattttcat tgataggtac agcgctgtgt tcacgcttaa
    16441 taatggtttg tgcatttaca cttgtaacaa gtaaaccaga aataaacagg ctaacaaaaa
    16501 ttcgcatccg gtgggttttc atacaataat acacgttgcg aagcttaaga tcgcaattgc
    16561 aaaagttaat tcatgttaat gctaaaaaaa gccggcattg agcgccggct tttcaagttt
    16621 tattcaaccg tgggtttacc gctcaaatag cccagtgaaa tgagaaactc atccgatttt
    16681 tttagcgcat cgcgataata ttttccgtct tcaaatttaa tggcagtaaa tccatgtttt
    16741 tgtccttcat aagtaaccaa ctcacagcga ttaccggctt tttcatagtc atcggcaaac
    16801 tgttttacaa actgatatcc tgaaatttta tcttttgttc catgaaaaat tatggtaggt
    16861 ggcattcctg ctcttatcga tgtcaatgga ttgatttcgg ctgcatgcga ttcgctatca
    16921 ggttgcttag catcatcctt aaatttttga atacgaaatt tatgctccat agaattcacc
    16981 acaggattga gcagtatcat cgcatttggt gcacatgaca cttgagcgtt gtctgttgaa
    17041 tcttgccatt tcggatcatt catattacaa gtcaaggcta aaaatgctcc ggcagaacca
    17101 ccggagacaa ttactttatt cggatctatg ttcaattcct tcgcatgttc acgcacatac
    17161 cgaactgcgc tctttgcatc aacaacacaa ttaatggcat tgcctttgtt tctggaaatt
    17221 acacggtagt cagcagttat ggcgacaatt ccgcggcttg caaaatattt acaatggtct
    17281 tcgtattgtc ccgggctacc atgaatgaat gcgccgccaa agaagtaaat aattacaggt
    17341 gttgcagctt gtttgtcatg ttttttagga tagtaaatat tcagatgcat ttcaacagaa
    17401 tctccgatct tcttgtaaac aggtgttgca tcggcactct caagaaccgg aacatagttt
    17461 tcctgcttac ctgcatcttc ctgcatctcg tctaatgatt ggtctttctt ctccaatttt
    17521 gacttacagg cacctaatag aagacttatc actgccaggg ataatgctat cttattcatt
    17581 tgtggttgaa atttgctgca aaaataccat aattaaatat acttttcatc taattgataa
    17641 tcaaatactt acactttccc aaaaaaaatt aaaaattgga ttattagatt tactttcaat
    17701 acataatatt caatatccca aaaaaataga ggcatttaca atcctacttg gtcaaaatca
    17761 catatcattt ttttataatc ttatgtaggc aattattaga ataacgaata aatttgtaat
    17821 atagaaataa attcattaat tttgaattca actttattgt taatttatat tcaatttata
    17881 aatttctaaa cttactactc ttatgaaaaa acacttggca caatttagct tattgctact
    17941 acttaccgtt ttgtcatcat gcgctaaaat ttattacagc ccagacgcat actcgcttgc
    18001 gcaaagccac cagatgatag ctgtcttgcc tccaactgtt tcaatagctg caagtaaaaa
    18061 aatagatgcg gaatcattaa aggaacaaca gaaaactgag tcacttaatt ttcaaaagga
    18121 aatgc
//
LOCUS       DAOQHY010000054        18067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1438_length_18067_cov_5.290751, whole genome shotgun sequence.
ACCESSION   DAOQHY010000054 DAOQHY010000000
VERSION     DAOQHY010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 18067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18067
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1438_length_18067_cov_5.290751"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="PLL28_06175"
     CDS             <1..619
                     /locus_tag="PLL28_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNF68944.1"
                     /translation="AGIGFAEQFKGTGNVCLCYFGDGAVRQGSVHETFNMAMLWKIPV
                     IFICENNGYAMGTSLERTTNQLDMYKIGLAYDMPSYPVDGMQPETVHAAIYEAAEKAR
                     NGEGPTFLEIRTYRYRGHSMSDPGKYRTKEEVEEYKLRDPLETTAQKILDKKYATQKE
                     LEDIQARIEEEIDACVKFADESPWPDDAAVYEDIYVQQDYPFLKD"
     gene            629..1321
                     /locus_tag="PLL28_06180"
     CDS             629..1321
                     /locus_tag="PLL28_06180"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF68945.1"
                     /translation="MSEVNKSATDTTLDKGTYEHVENFFLKNRNNILIALGVIVLAIG
                     GYFAYKQLYMSPRIATAQNNIAGAQNYFAKDSFDLALNGDGAVMGFLAIAENYGNTPS
                     GNMAHYYAGVCYMRKAELDNAIKHLEDFNPTTNEIAGEKYVLLGHAYADKKDYKKAID
                     MYKKAGDEAENNLQSPAFYKFAGDLMSEQNDLKGALEMYRKIKQLYPLSQEGQNIDLD
                     ITYAENKLGIKE"
     gene            1321..1812
                     /gene="ribH"
                     /locus_tag="PLL28_06185"
     CDS             1321..1812
                     /gene="ribH"
                     /locus_tag="PLL28_06185"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNF68946.1"
                     /translation="MATGKQHLTPQQEAAIPSGDQFRIGIVVSEWNTAITEKLYQGCY
                     HTLTHYKTPSEQIITVYVPGSFELPQAAQLLLESQELDAVICLGCIIKGETSHDQHIA
                     QAVAHGVIQVGLDYSTPVIFGVLTTNDEQQAIDRAGGKHGNKGEEAAITALRMAALRE
                     KLI"
     gene            1823..2587
                     /locus_tag="PLL28_06190"
     CDS             1823..2587
                     /locus_tag="PLL28_06190"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNF68947.1"
                     /translation="MKQLFWKWFIAVDRRLKFPSLKAGETGVQIGFDMSAPVTSDLFL
                     MVENVGPAGNVIGIDPDPRNIAAAHLIVARQRYPVTLIHRAVFNHEGKMGLLLGEKAS
                     WNQLTNVPIDDTVSFNGEEVVVEMDTLDNIFHQFNIDTHTIGHINITINGAEYFALLG
                     MHQILQKKDLALTVVAGRYDDSGMIDGEPDHEKIMALLKSYGFKVTFRRIHQMFWWGF
                     VINTLMKRRWIYGKDNYGIVMAAKGSKRIPWFQSFS"
     gene            2628..3485
                     /locus_tag="PLL28_06195"
     CDS             2628..3485
                     /locus_tag="PLL28_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNF68948.1"
                     /translation="MQIYSVHAGLFKLDGGAMFGVVPKKIWNKLVPADDNNLCTWAMR
                     CMVVVGGGHTVLIDCGMGDKQDEKFFGHYEPHGPYSLEASLKTHGITPGDITDVFLTH
                     LHFDHCGGAVKKNAKGKLIPAFENARYWSNEKHWAWAMQPNAREKASFLKENFQPLQQ
                     HGVLHFIEENEELFPGFNTMWTYGHTEAMMLPLIDFKGSKLLYCADLLPSHAHIPLPY
                     VMGYDVRPLVTIKEKEAIIKEAHDKKWWLFFEHDAEHACCNLITTEKGIRPDTFSTPE
                     DIPVLVKKR"
     gene            3572..3648
                     /locus_tag="PLL28_06200"
     tRNA            3572..3648
                     /locus_tag="PLL28_06200"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3606..3608,aa:Met,seq:cat)
     gene            3725..5200
                     /gene="rmuC"
                     /locus_tag="PLL28_06205"
     CDS             3725..5200
                     /gene="rmuC"
                     /locus_tag="PLL28_06205"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNF68949.1"
                     /translation="MELIFLIIGIVIGAVAAWLIRKYMSERDMVSVKELDAKQSALLQ
                     AENTIKYLQEKQNDLQASVAQAMQELRSAQTEKEQVQIQLATSNTSLQQLSQQLTDTK
                     NQAEQFANEINELKNFMIKVRGENEVLKEKNATIKKEIEELGEKSTMHFRNLANEILE
                     DKSKRFTEENQKNIKLILDPLNQDIRDFKKKVEDTYEKENKQRFSLEEKIRDLVSLNQ
                     RISDEANNLTKALKGQRKTQGDWGEMILENILQQSGLTEGREYAKQTTLRDESGNTLI
                     NEDGRRMQPDFIIYYPDKREVIIDSKVSLSAYERFSSADDDEAQKKALDEHVAAVYHH
                     IDELSKRSYQDFAQSLDFVMMFVPIEPAYLVAMQHDRDLWNYAYKKRILLISPTNLIA
                     AVKLVSEIWKKDEQNKNAIKIAERGAALYDKFVGFVENLQTIGDHIARSQRSYDDAMK
                     QLKDGSGNLIGQAEKLRKLGVSAKKTLPGTIIDESDVDDDQ"
     gene            complement(5201..6172)
                     /locus_tag="PLL28_06210"
     CDS             complement(5201..6172)
                     /locus_tag="PLL28_06210"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HNF68950.1"
                     /translation="MSDMKWRTEVMPDPLQTKIAVNAKCIFAGSCFAENLGAFLQQYK
                     FNVLVNPTGIIFNPIAIVNTLKTGASNTIPSQDRLFNHVGLWRHPDVHSNLAHPNRES
                     AWNLIKDAYRVLHQHFTQSSHIAVTFGTAWVYKELSTGKIVANNHKLPAAHFTKQLLS
                     VNEIVNTWQPFIAQFPEKQWIFTVSPVRHTRDGMTQNSRSKAILIESVNQLAENNNNV
                     YYFPAYEIMMDDLRDYRFYEQDLIHPNQQALQYIRSLFVQTCLDDHAAKWMAAFEPII
                     AARNHRPLFPDSQEHAAFKKSILLKLESLKKEYPIMHIAEELDYFAS"
     gene            6257..6676
                     /locus_tag="PLL28_06215"
     CDS             6257..6676
                     /locus_tag="PLL28_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68951.1"
                     /translation="MQFLHDRPHFVIVCEPGKGIAEMLTGYLHYYAGSSAPVSWYPAQ
                     SSIPTLVEQVLHEDGIHNWHPFNDENSGEPVVICINRVELPVTVKTILQRYQSEQITL
                     HLPTETSINIHQLRALREEIRQCAIMIMGKFSTTVMQ"
     gene            complement(6729..8591)
                     /locus_tag="PLL28_06220"
     CDS             complement(6729..8591)
                     /locus_tag="PLL28_06220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNF68952.1"
                     /translation="MKKLLFTLLVSLGCIGAANAQLPTATLTGEISGITRNLVNDTVY
                     ILNGFVYVEDGATLNIEAGTIIRGDKDTKGSLIVTKGSKIYANGTADQPIVFTSNQPE
                     GTRNYGDWGGLIILGNAPINVPGGTAVIEGGVDTPEGDGVYGGLDPLDNSGIIKYVRI
                     EYPGIAFVPGNEINGLTCGGVGAGTVIDHVMVSRSGDDAFEFFGGTVTAKYLIAHNAL
                     DDDLDTDFGFQGKIQFAVVKRDPNFADVSGSNGFESDNDATGSTNAPQTNPTFSNITV
                     VGPIQNAGDVINVNYKRGAHLRRNTSTDIFNSVIIGYPSGIMIDGALCEASATDNNLK
                     VQNTIIAGHNNDFEVAAGSTFDANAWFTTAAYNNTIYDLSSDVMLTDAYNATSPNFLP
                     MAGSPLLSGSSFAAPELAGFTNTTYRGAFGDLDWTSCWANWDPQNTNYGEVAGTIAPA
                     IASFSYANSDLTVSFSNTSSNAVSYIWDFGDETTTDDVSTEANPSYTYPALGVYTVTL
                     TATGTCADQNTTTNNLDLSVAITDASTLQSIMLYPNPASDVATLVINATEDMQGVVTI
                     LDMAGNMVMPAAEQHILSGKTTIQLATADLASGMYMVNILAQGAQQTLPLLIAK"
     gene            complement(8619..11468)
                     /locus_tag="PLL28_06225"
     CDS             complement(8619..11468)
                     /locus_tag="PLL28_06225"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNF68953.1"
                     /translation="MQRLIFLAFLLITATIAGKAQNGMISGVIIDGKTAETLIGANVL
                     IKEKAGTGSSTDIDGRYKIDDLEPGTYTVEISYISYDTKTVPGVEVKPGQVTSLDVTL
                     TSGLMELTVVEVKARPRQESLNTVLAFQQKNIAPSEVISRDIITRTPDKNTGEVLKRM
                     SGTTITDGKFVVIRGLSDRYNLALVNGNLLPSTEPDRKTFAFDLFPSAMLDNLIVIKA
                     AQPNLPGDFAGGIIILNTRDIPDKPFISISAGAGYNTISTFKEYYTYTGGKTDWLGID
                     DGTRQLPAGFPVSQDLFDDLTTTEKANFAKQMAPWGAYAKASSPLDQSYQLSGGWVKK
                     IGKVGEFGAIGALTYNNSNVSSFIKRNDFDNGTDSLYAYQDDQFKNNILWGGLLNLSW
                     KLNDNTRFSFKNSYNINSSDITTMRSGVNFSRAQYVQNEYYEFNSNELFSTQISGDHY
                     LTGSQVKLKWSLGLNTMKRDQPNYRSVNYAKNITPSFDGDTLFTISPSPFASPENLGI
                     FYSYMDEQTYTAGLDITKPFKILGTKQSLGFGGSYLTKARSFDARIMGVATANDIFFN
                     PDYFNIVSLPIGELLQPANFSDSLFYLDEITNPSDAYTATQENKAVYLMIDNQIANKL
                     RVVWGARAEFFNQTLQSFAYGFSSTPDTVEVDTKETDSVGLKFDFLPSINITYGVTAK
                     TNIRVSAYKTVARPELRELAPFGYYDIETNSSIVGNPSLKATNIYNADVKVEHFFGAG
                     QVISGGVFYKKFYDPIGQRFFFGTVRELKPINDSLATVVGAEFELRKNLGFISPKTKW
                     LEQFTFNTNLAVLNSTTVLHIADTAIAGTAERDMQGQSDYVINAGFTYIQPEKGYSCT
                     LLFNQIGRRISEYGNAQYDDIYENPRPLVDAQVSVPFNKGKGNVKLNMSDILNKDAVF
                     YQDVDGDGNYVAEKDNAIRVIDNGAKISLSVNYTF"
     gene            complement(11611..13995)
                     /locus_tag="PLL28_06230"
     CDS             complement(11611..13995)
                     /locus_tag="PLL28_06230"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNF68954.1"
                     /translation="MKKLLLGCLAFTGVLTAKSQVTPITVSGEITTNTTWTKNNIYLL
                     NGFVYVEDGATLTIEPGTLIKGDKDSKGTLIITRTGMINAAGTACEPIVFTSNQPAGS
                     RTYGDWGGVIILGEAPINVPGGTAVIEGGVDTPEGDGVYGGALPADNSGTFTYVRIEF
                     PGIPFLPGNEINGLTMGGVGSGTTINHIQVSYSGDDSFEWFGGRVNSKYLIAYRGWDD
                     DFDTDYGYRGKGQFFYALRDPNIADVSGSNGQEQDNDATGSANTPTSRPLFSNITIAG
                     PKVNTGDVVNVNYKRGAHDRRNTMASTYNSIIMGYPECGLKIEGVNTSLNAQNDSLQW
                     KKNILSGHGDDYICTTCDVGFNIDTWASTNGNATYATNALVGLVDPFNLSNPDPRPTA
                     GSAPTTNGTYFSGLLADPFFTATSYAGAFSTTNNWTDESWVNFDCQNTPYNTPGISNN
                     PVVSAVVTSMQCANTGAIDVSVSGGIGSYTYLWNDGATTQDRTGLNAGSYTVTVTSGT
                     CTTTATYTVSDITITKPTSLTTTQATHCSIKLNWAAVPGAGSYEVRYRITGGTYSSPV
                     NVGAVTSYTFTGLAASTSYDLQVRAKCPTGAEKSAYAKKTSSTVACGAPTSQSAGSIT
                     ANSAVISWSIPCSVTNYNLQYRKNPTKAWTTVSGIATTSYTLTGLLPSSTYEYRIASN
                     CGGTLSAYTGFSNFVTLPFRLEGEVEMPAIDADMEIYPNPAVDEVNMVINAQGVVNVI
                     ITNMLGQVVYNLENVEVNGTETVSLSLEGFDTGIYHVTIQNDVIKLTDQLVVSK"
     gene            14323..>18067
                     /locus_tag="PLL28_06235"
     CDS             14323..>18067
                     /locus_tag="PLL28_06235"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNF68955.1"
                     /translation="MKTFTLTTALAIITNLTFAQLPDQDYILDFIDPSITRSASGDIN
                     NDGIIDYVGGYYQIHWFEGNGTYEFGTIHNTGAKLNEYLTDLKVVDIDHDGWKDIWYQ
                     KGTVPFEMGWLKNPGATDQEWTTYSVAGSYRVCLGIFDMNGDGNEDFVFEDGAYLLGY
                     RELGDGNFSVYHAIPDSWSWVESFSAEDLDGDNDVDLAVYCYDSGVKYWRNNSNGTFT
                     STNLKNSESSYDVYSGDVNGDNHPDILYNNGSFYVSTFDTITHTFSPGVYNGNMDDFD
                     FRKFLNIDLDLDGDKDFVIVNDNYFAGESTYEISWLESDGSFLNLNVENFESIIAGLP
                     YYNSCMSFNDMNNDGTFDFIQCESGDYGTTAIVSPNIGGFFGAYDSSFLEVSPEVADL
                     IVYDVDGDPDNDLLLAFNNGLLSWFEFNHLVDSFTIMHNINYTGSRPTPENIKKKDID
                     SDGDFDIISSFSYNGALARTFYHLDLGDGNFETHTLCDTAFSNVWLIDGDEDGDFDLA
                     GLKAGDLNRLLYFTNTGNPDHLFSYTTELTAYGYYNSFVQLDIDDDGDDDLVGIRNDA
                     NGIKKIINLDGGLLFEDISNLFVSACDDVKTLFTSDFDNDGDKDFAYVCGDGTMHMAL
                     DNGTGYTDSAIGTFDPATLSDGSHTAMFDMNMDGLEDFVFNKTSGGSGFKLATGPGTF
                     QEDYDLFSTDYGRFANVDPGTLPDFVGISSNGLYIQYNILMLPPSFEVTPPTQTYISE
                     GAEEDSIIITFNTIPQEPLEINVLPYGILNAGAGESIGYTFIVDADSTALEPKVIHLS
                     TNEDAIAEDITIGGVTITADEVSWGAFASLLDEHYDFTITDNDLAIFTTNDALEINEG
                     IWSDTVFAHLNVIPQNDVLIQAIADPNYISAFGNDMTETYTIPSGIVGIAPFKMTLLY
                     PEDATYFGDIASSLTIELLSDDAAIDAFTSELLPVTLIENDVVDVQVVFGTTELSEGI
                     ESTTMTISLTSAPTQDVTITYTPDIYLDLGLGTGVPVSITFSGGDVIPSAQTLTVTAV
                     DNFFAEGTHDAMIIESIVSADIYYADITPASYIFSISDNDLPQGITVTLPPAATYMEG
                     TTDILMSMSLWTNPNALVTIIGHPDANIDLGLGAGIDNSISFDPLDDATIPRIFNITA
                     VNNTIIGPEITLANISFEIITTDPVLDDEIIEPAEISIQDEDMLQGIQIAAPPAIAYT
                     EGTADLTMSLMLNVEPNSAVTINCIPDAQLDFGAGAGVAVSVLL"
BASE COUNT         5457 a         3538 c         3816 g         5256 t
ORIGIN      
        1 agcagggata ggttttgcag aacaattcaa aggaacgggc aatgtttgtc tgtgttactt
       61 tggcgatggt gctgtgcgtc aggggtctgt tcacgagact ttcaatatgg cgatgttgtg
      121 gaagataccg gtgattttca tttgcgaaaa caacggttat gccatgggaa cgtcgctcga
      181 gcgcactacc aaccaattgg atatgtataa aataggtctt gcctatgata tgccaagtta
      241 tcctgttgat ggcatgcagc ctgaaaccgt tcatgcagcg atatacgaag cggcagaaaa
      301 agcgcgcaat ggtgagggtc ctacattttt ggaaatccgc acttatcgtt atcgcggaca
      361 ttctatgagt gacccgggaa aataccgcac aaaagaagaa gtagaagaat acaagctgcg
      421 cgatccgctg gaaacgacag cacaaaaaat attagacaaa aaatacgcta cgcagaaaga
      481 gcttgaagat attcaggcac gcattgaaga agagattgac gcatgcgtga aattcgccga
      541 tgaatcgcca tggccggatg atgctgcagt gtatgaagat atttacgttc aacaagatta
      601 ccccttttta aaagattaat atttcgatat gtcagaagtt aacaaatccg caaccgacac
      661 cacactcgat aaaggtactt atgagcacgt ggaaaatttc ttcctcaaaa atcgcaacaa
      721 catcctgatt gctttgggtg taattgtgtt ggctattggc ggttattttg cttacaagca
      781 attatatatg tcgccacgca ttgcaacagc acaaaataac attgcaggtg ctcagaatta
      841 tttcgcaaaa gattcatttg atcttgcatt gaatggcgat ggtgctgtga tgggatttct
      901 tgccattgca gaaaattacg gtaatacacc aagcggcaat atggcacatt attatgccgg
      961 cgtttgttat atgcgcaagg ctgaattgga caatgccatt aaacatctgg aagatttcaa
     1021 cccgacaacc aacgagattg ccggtgaaaa atatgtattg ttaggtcatg cgtatgccga
     1081 taaaaaagac tacaaaaagg ctatcgacat gtataaaaaa gcaggtgatg aagcagaaaa
     1141 taatcttcag agtcctgcat tctacaaatt cgcaggcgac ctgatgtcgg agcagaacga
     1201 tttgaaaggt gcattggaaa tgtatcgcaa gatcaaacaa ttatatccgc tttctcagga
     1261 aggtcagaat attgatctcg acattactta tgccgaaaac aaactcggta ttaaagaata
     1321 atggccacag gcaagcagca tcttacacca cagcaggaag cagctatccc ttcgggtgat
     1381 caatttcgaa tcggtattgt cgtttcagaa tggaataccg ctatcacaga gaaattatat
     1441 cagggttgct atcatacgct gacgcattat aaaacgcctt cggagcaaat aatcactgtt
     1501 tatgttccgg gtagttttga attacctcag gctgcacaat tattgcttga atcgcaggaa
     1561 ttggatgcag ttatttgctt gggatgtatc attaaaggag aaacttcaca cgatcaacat
     1621 attgcacagg cagtagcaca tggagttatt caggttggac ttgattattc aacgcctgta
     1681 atatttggtg tattgactac caatgatgaa cagcaggcaa tagatcgcgc cggcggaaag
     1741 cacggcaata agggcgaaga agcagcaata actgcattgc gcatggcagc gcttcgcgag
     1801 aaactcatct gaaaagattc gcatgaaaca attgttttgg aaatggttta tcgcagttga
     1861 caggcgattg aaatttccat cccttaaagc aggcgaaact ggcgttcaga ttggctttga
     1921 catgagtgca cccgtaacat cagatttatt cttgatggta gaaaatgttg gccctgcagg
     1981 aaatgttatt ggcatagatc ccgatcctcg caatattgca gctgcacatt taattgttgc
     2041 gcgtcagcga tatcctgtta cacttattca tcgagcagtt tttaatcacg agggcaagat
     2101 gggattatta ttgggtgaaa aggcaagttg gaatcaatta acaaatgtgc ctatcgatga
     2161 caccgtttct tttaatggcg aagaagtggt agtggaaatg gatacacttg ataatatatt
     2221 tcatcaattt aatattgaca cccacactat cgggcatatc aatatcacga tcaatggcgc
     2281 cgaatatttt gccttactgg gcatgcacca gattttacag aagaaggatt tagcattaac
     2341 cgtggtggcg ggaagatatg atgattcagg aatgattgac ggggaacctg atcatgagaa
     2401 aatcatggca ttgttgaagt cgtatggatt taaagtaaca ttccgtcgca tccaccaaat
     2461 gttctggtgg ggatttgtga ttaataccct gatgaagcgc aggtggatct acggcaaaga
     2521 caactacggc attgttatgg ctgcaaaagg aagcaaacgc ataccctggt tccagtcttt
     2581 tagctgaatg cgtagcatat taatctgata atcactactt tagccttatg caaatctatt
     2641 ctgttcatgc cggcttgttc aagctcgatg gtggcgccat gtttggggtt gttccaaaga
     2701 aaatatggaa taaattggtc cctgcagacg acaataatct ctgcacctgg gccatgcgct
     2761 gtatggtggt ggttggcggt ggacataccg tgctgataga ctgtggcatg ggcgataagc
     2821 aggatgagaa gttttttggt cattacgagc cgcatggtcc ttattcattg gaggcatcat
     2881 taaaaaccca tggtatcacg cccggcgata ttacggatgt atttctcacg catcttcatt
     2941 tcgatcattg tggtggagcg gtgaaaaaga atgcgaaagg aaaattgata cctgcatttg
     3001 aaaatgcgcg atattggtca aacgaaaagc actgggcatg ggcgatgcag cctaatgcaa
     3061 gagagaaagc cagttttctg aaagagaatt ttcagccatt gcagcaacat ggcgttttac
     3121 attttattga agaaaatgag gagttatttc cgggcttcaa tacaatgtgg acttatggtc
     3181 atactgaggc gatgatgttg ccattgattg actttaaagg cagcaagttg ttgtattgtg
     3241 ccgatttact tccaagtcat gcacatattc ccctacccta cgtaatgggt tatgatgtaa
     3301 ggccgttggt aacgataaaa gaaaaagaag ccattattaa agaagcgcat gacaagaaat
     3361 ggtggttgtt cttcgagcat gatgccgaac atgcctgttg caatctgata actacggaaa
     3421 agggcatcag acctgatact ttcagcacgc cggaggacat tccggtgctg gttaaaaagc
     3481 gctgatacgg tttttaaaac atcgataaat atgctacctt tgcacccgca acgcatcaat
     3541 ttatgctgtt tcaggcgtaa agcgggcacg cggcgtggta gctcagttgg ctagagcgca
     3601 gcactcataa tgctgaggtc gggagttcga gcctccccca cgctaccata gcaaccttcg
     3661 ggttgctttt tttattgcct caggcgaagt aattgtttag taacttcaag cccttgttta
     3721 gtgtatggaa ttgatatttc ttattatcgg aatagttatt ggcgccgttg ctgcctggct
     3781 gattagaaaa tacatgtcgg agcgcgatat ggtatctgtg aaggagttgg atgcgaaaca
     3841 aagtgcgctg ttgcaggcag aaaataccat aaaatatttg caggagaaac aaaatgatct
     3901 gcaagcttcc gttgcacagg ctatgcagga attgcgcagc gctcaaacgg aaaaggaaca
     3961 ggtgcaaatt caattagcaa catcgaatac ttctttacag caacttagtc agcaattaac
     4021 tgacacaaaa aatcaggctg aacaatttgc gaatgagatt aatgaattga aaaatttcat
     4081 gatcaaggtg cgtggggaaa atgaagtgtt gaaggaaaag aatgccacca ttaaaaagga
     4141 gattgaagag ctgggagaaa aatcaaccat gcatttcagg aatcttgcaa atgagatttt
     4201 ggaagataaa agcaagcgat ttaccgagga aaaccagaaa aacatcaaac tcattcttga
     4261 tcctttaaat caggatatcc gtgattttaa gaagaaggtg gaggacactt atgagaaaga
     4321 gaacaaacaa cgattttctt tggaagaaaa aatccgcgac ctcgtcagct taaatcagcg
     4381 catcagcgat gaggcgaata atcttactaa ggcgctaaaa ggtcaacgaa aaacacaagg
     4441 cgactggggt gagatgatcc ttgaaaatat tctgcaacaa agcggattaa ctgaaggacg
     4501 agaatatgcc aaacagacaa ccttgcgcga tgaaagcggc aatacgctga tcaacgaaga
     4561 tggtcgccgc atgcaaccgg atttcatcat ttattacccc gacaaacgcg aggtgatcat
     4621 cgacagcaaa gtttctttga gtgcttatga gcgattcagt tctgcagatg atgatgaggc
     4681 acagaaaaaa gcgcttgatg aacatgttgc tgcagtttat catcatatcg atgaattaag
     4741 taaacgcagt tatcaggatt ttgcgcaaag cctggatttt gtgatgatgt ttgtgccaat
     4801 agaacccgcc tacctggttg ccatgcagca tgatcgcgat ttgtggaatt atgcctataa
     4861 aaaacggatt ttactcatct cccctactaa tttgattgcg gctgttaagt tggtgagtga
     4921 aatctggaaa aaggacgagc aaaacaaaaa tgctataaaa attgccgagc gaggcgctgc
     4981 cttgtatgat aaatttgttg gctttgtcga aaatctgcaa accattggcg accatatcgc
     5041 cagatcgcaa cgcagttatg atgatgcgat gaaacagctg aaagacggat caggtaacct
     5101 tatcgggcag gcggagaaac tgcgcaaact gggtgttagc gcgaagaaaa cactaccggg
     5161 aactattatt gatgaaagtg acgtggacga cgatcaataa tcaggaagca aaataatcaa
     5221 gctcttcggc aatatgcatt attggatatt ctttcttaag cgattccaat ttcagcaata
     5281 tacttttttt aaatgctgca tgctcttgag aatccggaaa caaagggcga tgattgcgcg
     5341 ctgcgataat tggttcaaat gcagccatcc atttagcggc atgatcatct aaacaagtct
     5401 gaacaaataa ggagcgaata tattgcaatg cctgttggtt agggtgaata aggtcttgct
     5461 cataaaaccg ataatcgcgc aaatcatcca tcatgatttc gtatgccggg aaataataga
     5521 cattattgtt attctctgca agttggttaa cgctttctat taatattgct ttactacgag
     5581 aattttgcgt cataccatca cgtgtatgtc gaaccgggct gactgtaaat atccattgct
     5641 tctcagggaa ctgtgcgatg aatggctgcc aagtatttac aatctcattc accgataata
     5701 attgcttagt aaaatgtgca gcgggtagtt tatgattatt tgccacgatt ttccctgtgc
     5761 tgagttcttt ataaacccaa gctgttccaa aggtgacggc aatatgggag gattgcgtaa
     5821 agtgctgatg taaaacgcga taagcatcct ttatcaaatt ccatgccgac tcacggttcg
     5881 ggtgcgcgag attggagtgc acatccggat gtcgccacaa acctacatga ttaaacaatc
     5941 tatcctgcga aggaatagta ttcgaggcgc ctgtttttag tgtatttact attgcaatgg
     6001 gattaaaaat aatgcctgtt ggatttacca gcacattaaa tttgtactgt tgaagaaatg
     6061 cacctaagtt ttcagcaaaa caagaacctg caaaaataca tttggcattc actgcaattt
     6121 tcgtttgcag aggatcaggc atcacttctg ttctccattt catatcagac attgcgataa
     6181 ataatttgca atttgattac actaatttat atttactaca gatatcggca ttcaaagtta
     6241 attcataatt aagaaaatgc aatttttaca tgacagacca cattttgtga tcgtttgtga
     6301 acccggtaaa ggcattgctg agatgcttac gggatatttg cattattatg cgggttcatc
     6361 tgcacctgta tcctggtatc ctgcgcaatc gagcattcca acattggtag aacaggtttt
     6421 acatgaggat ggcattcata actggcatcc gtttaatgat gaaaattcag gcgaacctgt
     6481 agttatttgc atcaacagag ttgaattacc tgttactgtt aaaacaatat tacagcgata
     6541 ccaatcggaa cagataacgc ttcatttacc tactgaaact tccatcaaca tacatcaatt
     6601 gagagcatta cgtgaagaaa tccggcaatg cgcaattatg atcatgggaa aattttcgac
     6661 aacagtaatg caataaaaaa agaggcccgg tttccctgca gcctcttacc taattgcaac
     6721 tttcttattt atttagctat gagtaatggc aatgtttgtt gtgctccctg agcaagtatg
     6781 ttcaccatat acatgcctga tgcgagatca gcagtagcaa gttgaatagt tgttttacct
     6841 gatagaatat gttgctcagc tgcaggcatc accatatttc cggccatgtc caaaatggtt
     6901 acaacacctt gcatatcctc agttgcatta atcactaacg ttgcaacatc agatgccgga
     6961 tttggatata acatgatcga ctgcaaagta gaagcatctg taatggcaac tgaaagatct
     7021 aaattatttg ttgtggtatt ttgatctgca caagtaccgg tagctgttaa cgtaacggtg
     7081 tatacaccaa gtgcaggata ggtataagat gggtttgctt ctgtgctaac atcatcagta
     7141 gttgtttcat cgccaaaatc ccagatatag cttacagcgt tgctggaagt attgctgaat
     7201 gatacagtca gatcgctgtt cgcatacgag aaagatgcga tagcaggagc aatggtgccg
     7261 gcaacttcgc cataattcgt attttgcgga tcccagtttg cccagcatga agtccagtcg
     7321 agatcgccaa aagcaccacg ataggttgta tttgtgaaac ctgctaattc aggcgcagca
     7381 aatgatgagc cactcaacaa aggcgaaccg gccataggaa ggaaattcgg agatgtagca
     7441 ttataagcat ctgtaagcat tacatcgctg ctcaagtcgt aaatagtatt gttgtatgct
     7501 gcagtagtaa accatgcatt tgcatcgaat gtacttccgg cagctacttc aaaatcatta
     7561 ttatgacctg cgataatggt attttgcact ttcaggttat tatctgtagc actagcttcg
     7621 cacaaggcac catcaatcat tatacctgaa ggataaccta tgattactga gttaaaaatg
     7681 tcagtagaag tatttctgcg taaatgagca cctcgcttat agttcacatt aataacatca
     7741 ccggcgtttt ggattggtcc aacaacggta atattgctga atgtaggatt tgtttgcggt
     7801 gcattggttg atccggtagc atcattatcg ctttcgaatc cgttagaacc tgaaacatcg
     7861 gcaaagtttg gatcgcgttt tactaccgca aattgaattt taccctggaa tccaaagtca
     7921 gtatctaaat catcatccaa ggcattatgc gcaatcaaat acttagctgt tacggtacct
     7981 ccaaaaaatt caaatgcatc atcacctgat ctgctcacca ttacgtgatc aataacagtt
     8041 ccggcaccca caccaccaca ggtaagacca ttgatctcat taccgggaac aaaggcaata
     8101 cccggatatt cgatgcgcac atatttaata atacctgaat tatctaatgg atcgagtccg
     8161 ccataaacgc catccccttc aggcgtatct acaccacctt cgataacggc agtaccaccg
     8221 ggaacattga tgggtgcatt tccgagaata atcaaaccac cccagtcgcc gtaattacga
     8281 gtgccttcgg gttgattact ggtgaaaacg attggttgat cagcagttcc attagcataa
     8341 attttagaac ctttagtaac gatcagcgac cccttagtgt ctttatcgcc acggataatg
     8401 gttccggctt ctatattcag agtggcgcca tcttccacgt atacaaagcc atttaatata
     8461 tacaccgtat cattgacaag attacgtgta ataccggaaa tttcgccggt taaagttgca
     8521 gttggcaatt gggcatttgc agcaccaata catccaaggc taaccaataa tgtaaataac
     8581 agttttttca taacattgat ttgtttgtag ttcttgtatt aaaaagtata attgacagat
     8641 aatgaaattt ttgctccgtt gtcgatcaca cggatagcgt tatccttctc cgcaacataa
     8701 ttgccatcgc catcaacatc ctgatagaaa acagcatcct tgttgaggat atcgctcata
     8761 ttgagtttta catttccttt tcctttattg aaaggaactg aaacctgtgc atctactaag
     8821 ggtcttgggt tttcataaat gtcgtcatac tgtgcatttc catattcgga aattcgtctt
     8881 cctatttgat tgaataataa ggtgcatgag taaccttttt caggttggat ataagtaaat
     8941 ccggcgttga ttacatagtc agattgtcct tgcatatcgc gttctgctgt accggcaata
     9001 gctgtatccg caatgtgcaa cactgtagtt gaattcaata ctgccagatt ggtattaaat
     9061 gtaaattgct ctaaccattt agttttaggt gagataaatc caaggttttt acgaagttcg
     9121 aactcagcgc cgacaactgt agcgagtgaa tcatttattg gtttcaattc gcgaacagtt
     9181 ccgaagaaga atcgctgtcc gattggatca taaaatttct tatagaatac acctccggaa
     9241 atcacttgtc ctgccccgaa aaagtgttcc accttaacat cggcattata aatgttagtt
     9301 gctttcaacg atgggtttcc aacgatgctt gagtttgttt caatatcgta ataaccgaat
     9361 ggcgcgagtt cacgcagttc aggacgagca actgttttat aggcacttac cctgatattg
     9421 gtttttgcag ttacgccgta tgtaatattt atggatggca ggaaatcaaa tttcaaacct
     9481 acgctgtcag tttctttagt atcaacttct accgtatcag gagtggatga aaaaccatat
     9541 gcaaaggatt gcaaggtctg attaaagaac tcagctcttg ctccccatac cacacgcaat
     9601 ttatttgcta tttgattatc gatcatcagg taaacagcct tattttcctg cgttgcggta
     9661 taggcatctg aaggattggt aatttcatcg agataaaaca ggctatcaga aaaatttgca
     9721 ggttgcaaca attcgccaat aggcaatgac acgatattga aataatcagg attaaaaaag
     9781 atatcgttag ccgtagccac tcccattata cgggcatcga aagaacgagc tttagttaaa
     9841 tagctgccac caaaacctaa agactgtttc gtgccaagaa ttttaaaagg tttagtgata
     9901 tccagtcctg cagtataagt ttgctcatcc atgtaggaat aaaatattcc aagattttca
     9961 ggcgatgcaa atggggaagg tgaaatggtg aataaggtat caccatcgaa gcttggtgta
    10021 atattttttg cataattcac cgaacggtaa ttaggctgat cgcgtttcat ggtattcaaa
    10081 cccaaagacc atttcaattt aacttgtgag ccggtcaaat agtgatcacc acttatttgc
    10141 gtactgaata attcgttaga gttgaattcg taatattcgt tttgaacgta ttgcgcgcga
    10201 ctgaaattca caccactgcg catggtagtg atatcgctag aattgatatt ataggagttt
    10261 ttgaatgaga atcgcgtgtt gtcatttaat ttccaactaa ggttcagtaa tccaccccat
    10321 aaaatattgt ttttaaactg atcatcctga taagcataaa ggctatcggt tccgttatca
    10381 aagtcattgc gtttgataaa acttgagaca ttactattgt tataggtcag tgcgccaata
    10441 gccccaaatt cacctacctt accaatcttc ttcacccatc cgcctgacag ttgataagac
    10501 tgatctaatg gagatgaagc tttggcatat gcaccccaag gtgccatttg tttagcaaaa
    10561 tttgcctttt cagtagttgt caaatcgtca aataaatcct gacttaccgg aaaccctgca
    10621 ggtaactgac gcgtgccatc atctatgccc agccagtcag ttttaccacc ggtataggta
    10681 taatattctt tgaaggttga aatggtgtta tagcctgcac cggctgaaat agaaataaat
    10741 ggtttatccg gaatatcgcg cgtgtttagt atgatgattc cacctgcaaa atcgcccggc
    10801 aaattcggtt gtgctgcttt aataacaatt agattatcca acatagcaga tggaaacaaa
    10861 tcaaatgcaa atgtttttcg atcgggctca gtggaaggaa gaagatttcc attcacaagt
    10921 gcaagattat atctatcgct aagacctctt attaccacaa acttgccatc agtaatagtg
    10981 gttccactca tgcgttttaa tacttcaccg gtatttttat caggagttct tgtaatgata
    11041 tcgcgggata ttacttccga aggagcgata tttttttgct gaaatgccaa aacagtattg
    11101 agcgattctt gtcgcgggcg agctttcact tcaacaactg tcagctccat caaacctgat
    11161 gttaatgtta catctaagct tgtaacttgt cccggtttta cttctacacc cggaactgtt
    11221 ttggtatcat aagagatgta gcttatttca acggtatagg tgcccggttc aaggtcatca
    11281 attttatatc ttccatcaat atcagtgcta ctgcctgttc cggctttttc ttttatcaat
    11341 acgtttgcgc caatgagcgt ttcagcagtt ttgccatcga taatgacccc tgaaatcatt
    11401 ccgttttgtg ctttccccgc aatagttgca gttatcagga gaaaagcaag aaaaattaaa
    11461 cgttgcatac aattaggatt ttgatgcaaa gttcagggtg tcatcttacg taattattag
    11521 gttaacatta gacttgtgtc aaacccatgt aaacgaaaaa tggcccctgc gttaacagga
    11581 ggccatttac tttaaactac aattaacatt ttatttactt actactaatt gatcggtgag
    11641 tttgattaca tcattctgaa tagtaacgtg gtagatacct gtatcaaatc cttcaagaga
    11701 aaggcttaca gtttctgtgc catttacttc aacattttca agattgtaaa caacctggcc
    11761 taacatattg gtgataatca cattcacaac gccttgcgca ttgattacca tgttaacttc
    11821 atcaactgca ggattcggat agatttccat atctgcatca atggcaggca tttcaacttc
    11881 accttccaaa cggaatggca atgttacgaa gttggagaaa cctgtataag ccgataatgt
    11941 tccaccacaa tttgaagcaa tgcggtattc ataggtcgag ctaggtaaca aaccggttaa
    12001 tgtataagaa gtagttgcaa tacccgatac tgttgtccaa gctttagttg gatttttacg
    12061 gtattggaga ttgtaatttg ttacggagca aggaattgac caactaataa cagcagagtt
    12121 tgcagtaata gaacctgcag actgcgatgt aggtgcacca catgctactg ttgaacttgt
    12181 tttctttgca taagcagatt tttcagctcc ggtaggacat ttcgcacgta cttgcaaatc
    12241 ataagaagta gaagctgcta atcctgtaaa tgtataagat gtaacagcgc caacgtttac
    12301 aggtgatgaa taagtaccac cggtaatacg gtaacgcact tcatatgaac ctgctccggg
    12361 cactgcagcc caatttaatt taattgagca atgagtagcc tgtgtagtgg taaggcttgt
    12421 tggtttagtg attgtaatat cagatactgt gtaggtagca gttgtagtac atgtgccgct
    12481 ggttacagtt acagtatatg aacctgcatt caatccggta cgatcttggg ttgttgcacc
    12541 atcgttccac aaataggtat atgaacctat tccgccgcta acactcacat caatagcacc
    12601 tgtgtttgca cattgcatgc ttgtaactac tgctgataca acaggattgt tgctaatacc
    12661 aggcgtatta taaggagtat tttgacaatc gaaattcacc catgactcat ctgtccagtt
    12721 atttgttgta gagaaggctc ctgcatatga tgtcgcagta aaaaatggat ctgccaacaa
    12781 acctgaaaaa taagttccat tagttgttgg tgcagaaccc gctgttggac gtgggtctgg
    12841 attgcttaaa ttgaaaggat caacaagacc tacaagtgca tttgttgcat atgttgcatt
    12901 tccatttgtt gatgcccaag tatcgatatt aaaaccaaca tcacaagtag tacagatata
    12961 atcatcacca tgacctgaaa ggatgttctt tttccattgc aaagaatcat tttgggcatt
    13021 gagtgaagta ttcacacctt caattttcaa tccacattca gggtaaccca tgataataga
    13081 attataggtt gaagccatag tattgcgacg atcgtgagca ccgcgtttgt agtttacatt
    13141 cactacatca cctgtattta cttttggacc tgcaatagtg atgtttgaga ataatggacg
    13201 gctggtaggt gtgttagcag aaccggtagc atcattatct tgttcctggc cgtttgatcc
    13261 ggaaacatcg gcaatattcg gatcgcgtaa agcgtagaaa aactgtcctt taccacgata
    13321 accgtaatcg gtatcaaaat catcatccca tccgcgatat gcaattagat attttgagtt
    13381 tacacgtcca ccgaaccatt caaatgaatc atcgccggaa taagaaacct ggatgtggtt
    13441 aatagttgta cctgaaccaa caccacccat ggtaagtccg tttatttcat ttcccggcaa
    13501 aaatggaatt ccggggaatt cgatacgcac ataggtgaag gtacctgagt tatcagcagg
    13561 aagcgcacca ccataaacgc catcgccttc aggcgtatca acaccacctt caataacagc
    13621 tgttccaccg ggaacgttaa ttggcgcttc accaagaata atcaccccac cccagtcgcc
    13681 ataagtacgc gaacccgcag gttgatttga cgtaaatacg attggctcac aagcagttcc
    13741 tgcggcattg atcataccgg tacgggtaat aattaaggta ccttttgaat ctttgtcgcc
    13801 tttgatcaat gtacccggtt cgatggtcaa ggtagcacca tcttccacgt acacaaatcc
    13861 gtttaagaga taaatattgt ttttagtcca ggtggtattt gtcgtgatct cgccactgac
    13921 agtgataggt gtcacctgcg acttggctgt caatacacct gtaaaggcga gacatccgag
    13981 taatagtttt ttcattgata agttctttta gaagtgatga aaagatgggg gcaaaagtag
    14041 atggggcgta ttagcggaat attgaggtag ttttagatgt ttcttagtat tttattacgg
    14101 taataaagcg atttcttaat tgaatgtcaa cagcgtagcc tcattgttaa tgctaggttg
    14161 atgtaggcta ataaaatgtt aatttggggt aaagtgcacc aaacccacaa tagtcactct
    14221 ctaagtcaga actaggtttt acaggcaaaa caggtcattt cactcaaagc gtcttgtgag
    14281 gacaagcagt agccaccata tttacattgt caattcaaac aaatgaaaac ctttacacta
    14341 accactgctc ttgcaatcat tactaaccta acatttgccc aacttcctga tcaggactac
    14401 atattggatt ttattgatcc gtccatcact cgttcggcct caggtgatat caataatgat
    14461 ggtatcatcg actatgtagg cggatactac caaattcatt ggtttgaagg caatggcacc
    14521 tacgaatttg gcacgataca caatacagga gccaagctga atgaatacct aacggattta
    14581 aaggtggtag acatagatca tgatggatgg aaagacattt ggtaccaaaa aggcaccgtt
    14641 ccttttgaaa tgggatggct caaaaatccg ggagcaactg atcaggaatg gacaacatat
    14701 tcagtggcag gttcctatcg agtttgttta gggatctttg atatgaatgg cgatggcaat
    14761 gaggattttg tatttgaaga cggcgcttac ctgcttggtt atcgcgaact gggcgatgga
    14821 aatttttcag tttatcatgc gattccagat tcttggtcat gggtagaaag tttttctgcg
    14881 gaggaccttg atggcgataa tgatgttgat ttagcagttt attgctatga tagtggcgta
    14941 aaatattgga gaaacaatag caatggcaca tttacatcaa ccaatttaaa aaactccgaa
    15001 tcaagctatg atgtgtattc cggagatgta aatggcgata accatcctga tattttatat
    15061 aataacggaa gtttctacgt aagcacattt gataccatca cccatacctt cagtcccgga
    15121 gtatacaatg ggaatatgga tgactttgat tttcgcaaat ttttaaatat cgacttagac
    15181 cttgatggag acaaagattt tgtgattgta aatgacaatt attttgcagg agaaagtact
    15241 tatgaaatct cctggctaga atctgatggt tcatttttga atctgaatgt cgaaaatttc
    15301 gaatccatca ttgcaggatt accatattac aatagctgta tgagtttcaa cgatatgaat
    15361 aacgatggaa cctttgattt tatacaatgc gaatcgggag attatggcac aacagccatt
    15421 gtaagtccaa atataggagg tttcttcggt gcttatgatt cttcattttt agaagtaagt
    15481 cctgaggtag ccgaccttat cgtatatgat gttgacggag atcctgataa cgacctttta
    15541 cttgctttca acaatggatt attgtcatgg tttgaattta accatttggt ggattcgttt
    15601 accattatgc ataatatcaa ttacacagga agccgcccaa cacctgaaaa cataaagaag
    15661 aaagatatag atagcgacgg cgatttcgat ataatctcca gtttttccta taatggcgca
    15721 ttagcccgaa ccttttatca tctcgattta ggcgatggca acttcgaaac tcacaccctt
    15781 tgcgataccg cattttcaaa tgtatggtta attgacggag atgaggatgg cgactttgac
    15841 cttgcaggtt taaaggcagg tgatcttaac agattattgt attttacaaa taccggaaat
    15901 cctgatcatt tatttagcta cacaacagaa ctgactgcgt atggttatta caactctttt
    15961 gttcaattag atattgacga cgatggtgat gacgatctag ttggaatccg caatgatgca
    16021 aatggtatta agaaaattat taatctggat ggaggattgc tgtttgagga tatctccaat
    16081 ctctttgtat cggcttgtga tgatgtcaaa acgctattta ccagcgattt tgataacgat
    16141 ggggataaag attttgccta tgtatgtggt gatggtacaa tgcatatggc acttgacaat
    16201 ggtaccggat atacagattc tgcgataggc acttttgacc cggccacact ttctgatggt
    16261 tcacatactg caatgtttga tatgaacatg gacggacttg aagattttgt attcaataaa
    16321 acttcaggtg gatcaggatt taaattagct acaggccccg gaacatttca ggaagactat
    16381 gaccttttct caacagacta tggcaggttc gcaaatgtag atcccggaac gcttcctgac
    16441 ttcgtaggta tctcctcaaa tggattgtat attcaatata acattttgat gttacctcca
    16501 tcatttgaag ttacaccacc aactcaaaca tatatttctg aaggtgccga agaagattct
    16561 atcataataa catttaatac tatccctcaa gaaccactgg aaataaatgt gctcccatac
    16621 gggatattga atgccggtgc cggagaaagt ataggataca ccttcattgt tgatgccgac
    16681 tcaaccgcgc ttgagccaaa ggtaattcac ttatctacaa atgaggatgc tattgcagaa
    16741 gatattacta ttggcggagt taccattaca gcagatgagg tttcatgggg agcttttgcc
    16801 tccttacttg atgagcacta tgacttcaca attacagaca atgacttagc tattttcacg
    16861 accaacgatg ctttggaaat aaatgaaggt atttggtcag atactgtatt tgcacattta
    16921 aatgtaatcc cacaaaacga cgtgcttatt caagcaatcg ccgacccgaa ttatatttca
    16981 gcatttggaa atgatatgac ggagacatat actatccctt caggaattgt tggtattgca
    17041 ccatttaaga tgactttgtt atatcctgaa gacgcaacct attttggaga tatcgcttca
    17101 tcattgacaa ttgaactttt atcagatgat gctgcaattg atgcttttac gagcgaatta
    17161 ttgccggtca cacttataga aaatgatgta gtagatgtgc aggtagtttt cggaacaaca
    17221 gaattgtcag aaggtataga atcaaccaca atgactattt cgttaacatc cgcacctacc
    17281 caagatgtaa caatcaccta tacacctgat atttatcttg atttaggctt gggtacaggt
    17341 gttcctgttt caattacttt ttcgggaggc gatgttattc cttcagcaca aacacttact
    17401 gttactgcag tagacaactt ctttgccgag ggcactcatg atgcgatgat aattgaatcc
    17461 attgtatcag ccgatattta ttatgccgat ataacaccag cttcctatat tttctctata
    17521 agcgataatg accttcctca gggaattaca gttacacttc cacctgcagc tacttacatg
    17581 gaaggaacaa ccgacatttt gatgagtatg tctttatgga caaatccgaa tgcattagtt
    17641 accatcatag gtcacccgga tgctaatata gatcttggtc ttggtgcagg aattgacaat
    17701 agtatttctt tcgatccttt ggatgatgct actattccca gaatttttaa tatcacagcc
    17761 gtaaataata cgattatagg tcctgaaatc acgctggcga atatatcttt cgaaataatt
    17821 acaacagatc ctgtattgga cgatgagata attgagcctg cagaaatttc aattcaggat
    17881 gaagatatgc ttcaaggcat acaaatagcc gctccaccgg cgatagcata cactgaaggc
    17941 acagcagatc taaccatgag ccttatgcta aatgttgaac caaattctgc agtaactatt
    18001 aattgtattc cggatgcaca attagatttt ggcgcaggtg ctggagttgc cgtatcagta
    18061 ttattga
//
LOCUS       DAOQHY010000055        18011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1443_length_18011_cov_5.450769, whole genome shotgun sequence.
ACCESSION   DAOQHY010000055 DAOQHY010000000
VERSION     DAOQHY010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 18011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18011
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1443_length_18011_cov_5.450769"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PLL28_06240"
     CDS             complement(<1..231)
                     /locus_tag="PLL28_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68956.1"
                     /translation="MLRYMKTALCTIFLMMILVSAVKADPGDTIKVQTFTFGSPQDAW
                     FDLPGLDVPVEKILMKYTLKCNPAQSPACGEWD"
     gene            311..967
                     /locus_tag="PLL28_06245"
     CDS             311..967
                     /locus_tag="PLL28_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF68957.1"
                     /translation="MVTATGIYKSFGPLQVLKGVDLQVNPAEVVSIVGASGAGKSTLL
                     HIIGTLDKPDKGDVILNDVSVKSLSEKQLSAFRNKLIGFVFQFHHLLPEFTALENVCI
                     PGYIAGRKESEVEARAKELLEYLRVSDRMQHKPNQMSGGEQQRVAVARALINQPAVIL
                     ADEPSGNLDTQTAKELHELFFQLRDTFKQTFIIVTHNTELADMADRKLVMQDGQMVLG
                     "
     gene            complement(980..2161)
                     /locus_tag="PLL28_06250"
     CDS             complement(980..2161)
                     /locus_tag="PLL28_06250"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNF68958.1"
                     /translation="MQAAGINRSSIQFKGKRVPVYTGSFWTSKQREGHNLHEIAYRAC
                     FKPSLPKYFIEKYTNEGDVVYDPFSGRGTTVIEAALRGRRIIANDVNPISAILSGGRL
                     CIPELQALDQQLSHILSGTPVKEQHTLSMFYHKHTLNEILLLREWLKKRKLSGTEDAL
                     DRWIRMVATNRLTGHSPGFFSVYTLPPNQAVSRERQLLINKKRNQKPEYRDVHALIMK
                     KSRNLIKDLDISTKKYLSQAATSALLINSDAAATRQIKNNSVTLVVTSPPFLDVVQYA
                     KDNWLRCWFNDIDVNEIAKRITMSKTVDAWMQKMQQVFFELYRITAKGGHVAFETGEI
                     HYGKTRLETIVLEMGLHAGFEVDRLMINEQQFTKTANIWGIRNNTHGTNSNRIVLFRK
                     K"
     gene            2276..2815
                     /locus_tag="PLL28_06255"
     CDS             2276..2815
                     /locus_tag="PLL28_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68959.1"
                     /translation="MQNTLPFGNVGDITFTPSGQDLKIYVSGQLLGTFNSSSPATVFI
                     TQNGSNKAITFKQISQSAYMQGNTGTPGVDTIYFNPLTGNSKIEACSGGAGCFKLTGI
                     GWEDDDYGQTCTGGSKGFTNLFNISAEGQLKAKFEQVSINGSMQIRLVARYSGNVMCK
                     QTPATAICCNDNYCYYCNE"
     gene            complement(2825..4345)
                     /locus_tag="PLL28_06260"
     CDS             complement(2825..4345)
                     /locus_tag="PLL28_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68960.1"
                     /translation="MHSPAEDLYHLIHALSANEKRYFRLFAQRHGNEKDKLYMKVFDL
                     IMAQEDGYDEEIVKKKLKTAAAIKQLPQTKNYLYDLIMQSMRLYRSDKNVATEVFNLI
                     QDELFYTEKGLTQMRSKALKKAKELAYEYDLIYCLVTILQRERIYALKFSGGDPNETI
                     RKLHAEEEWVFDRLNNESKLGEVFFTLWVQFIKDPLMRSNEDLEQYTSSRDAVLNQKF
                     EDLHTFTEKFFWLRAKGLICKYNHDYQGTFQLTKQLVDLFSDYPQHRLNVMGNYIDAL
                     SNYLSAAHVVGNYDEFEEVIAKLEELPREAVKDDNSISQVVINNKILYAMNRGMYSRS
                     GMLAEEFENYCRKFENIITESFLVVTPYNLSLMFFINGEYDRSLDYCQRVMQFKTDVK
                     IELQHGASMLYLIILIEQEQLVFFESAYRSVVRSMKNRGKFDDFERLFTTQLKKIANT
                     PERQRKPLYGKLYEDLLVLKKESGNKRFVFMTETLSWCASKTGDKKMAELVLQPFN"
     gene            4671..4931
                     /locus_tag="PLL28_06265"
     CDS             4671..4931
                     /locus_tag="PLL28_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNF68961.1"
                     /translation="MHAPILFTKKRLLDFSKRERQILQLIAKGMTTREIALELSISEN
                     TVESHRKRMIRRVEARNIFGVFHYTISNGILQPSVFSDPYLL"
     gene            5052..5714
                     /locus_tag="PLL28_06270"
     CDS             5052..5714
                     /locus_tag="PLL28_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68962.1"
                     /translation="MEIFAYIFYIGVINIVFTSGWKLLATMVGSLLKDVGVNKDNTFL
                     VFKTVGYYILVSLAALVTAQHMETTYKLAAMAYAFTGTFVIYATIASNLERNRWRAVM
                     NFERKRIQVMRYDGYLLMACIVLFIATILQPQIADLAPHRWLLETIDSIYNTIFLKWV
                     IGFFAFFYMLNIIFRGIKFSDEVFAMLFNRKRPGSDNNDNSGTEYADYEEIIDDDSRL
                     PK"
     gene            5748..7952
                     /locus_tag="PLL28_06275"
     CDS             5748..7952
                     /locus_tag="PLL28_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF68963.1"
                     /translation="MRQILTIFLLCLSQLLFAQDVQWASEVVKFSTEYSRTQFSAKQA
                     LGAPNKLPASGESTVAWAPSTPDNPAGEFIQVRFAQPQYVQQIGIGESNAPGAVKEVV
                     LISTEGKKYTVYTNDTIKPAYNTGSRVFRIFIPRTDYLVKEVKVSLNTKVIAGMNQID
                     CIGISDSREPIKAKINELAYAEKLPPPENLGTKVNSDADDMFPFISPDGKTLYFTRKN
                     YFENTGPDLRDDIYVSKLDNDKWTTAINIGPPLNNEQHNFVSWISPDGSSLLLANNYQ
                     KIKQDISISTKTGTGWAFPKTLKINDFYNNNEFSCYHMNTEGNVILMAIERPDTHGDM
                     DIYVSFLQANKVWTEPKNIGGTINTAATEGSIFIAADNRTIYFASNGHSGYGGYDMFM
                     SKRLDDTWTNWSEPLNLGSQINSTGNDYYFTIPASGDYVYFSSTNNSYGKADLYRIPL
                     PKPLQPDPVTLLKGTIIDAETKKPIPADINFGGLVSEEPDGISTSGDGGYQVVVPESN
                     YLISIIKPGYLPYSATIDPELHYDAIDYNPEDSIATIKHEITTTLKAEIKTNPITPEA
                     LEEEVIKLLPDEIADTSSTVRDSIIRDITDAFETKDSTYKEVTADIEMIPIREGTVIR
                     LNNIYFEANKSDLRPESSSALDELVTFLKTNPNIYVEIGGHTNGLPSDGFCQKLSNDR
                     ANKVVEYLIANGIPADHLTWKGYGKTQPIADNATVTGRRQNQRVELKVMKVE"
     gene            7992..9704
                     /locus_tag="PLL28_06280"
     CDS             7992..9704
                     /locus_tag="PLL28_06280"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68964.1"
                     /translation="MSSLSFHVFMDNPHTHTFTIKMHIPAMGKDVLSLALPVWTPGSY
                     LVREYSKNINRLYCEDKNVGINKTAKNTWEITTNNQAIDIIYELYAFEESVRTCWLDS
                     ERASIIPAGMFLFIEDTPLPVNISFHPHHAFKDIATSLPMVNNDPWVRFAADADTLYD
                     SPIEIGNHITQHFNAAGVEHTLVISGTGNYHIPSMITDMQAVIETEVNIFKHHPCKSY
                     LIILNTSNTLRGGLEHLHSTSLIFKRLMFADRSNYQEFISLFAHEYFHLWNVKRLRPK
                     ALGPFNYIAENYTTSLWIAEGFTSYYDDLIVYRTGLYAEEEYLTIVEKNINLAENAPG
                     KKIMSVAEASFDAWIKYYRQNENSENSQVNYYVRGGVLATLLDILIIHHSDGKHNLDN
                     VMRTAYERFYVNRDSGYTEDEFKAVIEEFAGISMNDFYRQYVFGTSQIAFNDILCHVG
                     LQLENTLENTIESDLGITINNNNIITAVRRNSAGEQAGIYVSDEIIAINGYRYSNGLL
                     ASLTADAKTGDKLEIILSKSGRMHTVSATIQITDKVNYRLKAMDDANDSQRAHYAKWL
                     LKNK"
     gene            9710..10225
                     /locus_tag="PLL28_06285"
     CDS             9710..10225
                     /locus_tag="PLL28_06285"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF68965.1"
                     /translation="METLHTERLTLSPCTIDDAAFLLALLNSPGWLKYIGDRNVHTLE
                     EARSYMQNRIMKAYEKDDYGMWLVRDTTTGAAMGLCGIVVRDTLPHPDLGFAFLPEFQ
                     GKGFAFEAAQAVMEFAIGYTDIDTMLAIATPDNTHSIKLLTKLGMSFESEFTDPHTKD
                     LLVRYSINLNI"
     gene            complement(10448..11203)
                     /locus_tag="PLL28_06290"
     CDS             complement(10448..11203)
                     /locus_tag="PLL28_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNF68966.1"
                     /translation="MAIVMITGASSGFGAATAEIFAQNGYRLILNARRIERLETLKRM
                     LSETYSADVLLLPFDVRDKDAVATAMDTIPASWKQIDVLINNAGLAAGRDLFQDGLVD
                     DWEAMIDTNVKGLLYVTKHAVSGMKERKSGHIINVSSLAGQEVYPMGNVYCATKHAVT
                     ALSKGLRMDLLPYHIKVTNISPGLAETEFSMVRFKGDEAKAKAVYEGFDPLQAKDIAD
                     VIYFAASRPQHMNLEEITILPTAQSDSRTVVRG"
     gene            11371..13551
                     /locus_tag="PLL28_06295"
     CDS             11371..13551
                     /locus_tag="PLL28_06295"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF68967.1"
                     /translation="MKQAILLFIGISSITALTAQTTISGTVTDNKKNLLPGANIFLKD
                     TYDGTTSNVDGTYSFITTETGKQILSVSFVGYESFEDTLQLNGGTLSVNIVLKEAFNE
                     LNAVVITAGAFEASDEKKNTILKPLDIVTTAGAEGDIYGALETLPGASKVGNDDGLYV
                     RGGSDYETKTIIDGMVVNNPYYSTVPDVPSRGRFQPFMFKGTVFSTGGYSAEYGQALS
                     SAVILNTEDMPEYSASGLSVAPIFLGGFHTQKFKNEKTALGGGINYTNLGLFDNVFKP
                     TTFDWINSVESVDGSLFFRQKTSKTGILKLYAQKESSNMALRAPDIDSLPATDDVYLG
                     NNYNYINASWREILTKKWGVQAGVSYSKNNDDILVDGFNVGRSDQNAQGKVTFNNEIT
                     EKIKLRYGGEFQHITYDQFAGDLEFDATDNFTAAFTESDIFITNDLAGRIGVRAEYSE
                     ILDDVNVAPRISLAYKTGKNAQVSLAYGDFYQTPQPNYLYTQALYPLQYEKATHYIAN
                     YQYITNDRTFRIEGYYKTYDQLVTLTTDSLNPFVPITANNGSGYARGIDLFYRDKKTI
                     DYADFWISYSFLDTKRKFLDYPEEVTPTFAAQHILSLVYKHYFPGINTQVSGTYRYIS
                     GWPYYNPNNPEFLGDVSPDLHNVALSASVLQSLWGNFTVFFISWDNTFGFDQIYGYNY
                     SADGSQFLVNQAPVKSTVFFGIFMSILYGKDKNVDDIDIDKSNK"
     gene            13596..14273
                     /locus_tag="PLL28_06300"
     CDS             13596..14273
                     /locus_tag="PLL28_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68968.1"
                     /translation="MKKILLVTLVALTSIGAQAQTAKFHDGMKKALTLMDSAKTGDDF
                     MNVANSFERIAMAEKNQWLPYYYSALARTTALFIYNDVTRNDAVLDVAEKHAMKADSL
                     SPNNSEIFVLRSMIFGGRIMVDPMSRGQMYGMQSMMLLQQAMTADPNNPRSYYIMGQS
                     LFYTPPQFGGGKEPGCKMFETALQKYSIFVPQSDLHPNWGLDQVNQMLSQCSATTDPN
                     TGKSGGN"
     gene            14890..16353
                     /locus_tag="PLL28_06305"
     CDS             14890..16353
                     /locus_tag="PLL28_06305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68969.1"
                     /translation="MKKLFLSLSLAVVAILGFNTSAKAQLEDGSVAPDWTLTDINGTT
                     WNLYTLLDEGYDVFLDFSAVWCGPCWAYHTGGSLEGLYDAYGPDGTNEVMVFYIEGDG
                     ASTLDELNGIGAGTQGDWVTGTPYPIVLTHAGDPSYSAVQDYDIAYFPTIYRVCQNRI
                     VDEVGQLSTAALYATISDCAAASVNVDPSILTYNGPVASCGDVPVSVTIQNMGFNELT
                     ACTITAYADGIEVASVDWTGSLNTYEIATVDVGTFTPADADVDLSVEITSADENTVNN
                     VIATTVTYEDNITQIIHLELKTDSYPTETKWEVIDESTGDVVAEGGPYTNAQKNEIVF
                     DEDIVLPSLGCFTFTCYDAYGDGITGSGYYKLYDNNGDLIQQGMENIGFEKSAALKVT
                     TAVAIEENSAINNVSLYPNPANDNINVDINLTATADVDVTIVNMYGAVVGTATSGTLT
                     AGAYSFTVNTADLANGMYFVQITGENVNNTVKFTVFH"
     gene            16545..17999
                     /locus_tag="PLL28_06310"
     CDS             16545..17999
                     /locus_tag="PLL28_06310"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF68970.1"
                     /translation="MKKITYPCILLIISLMMVAPAKAQLEDGSIAEDWTLTDINGTQW
                     HLYDILNQGKSVFLDFSAVWCGPCWTYHNSGNLEALYDTYGPDGTNEVMVFYIEGDGA
                     STLDELNGIGAGTQGDWVDGTPYPIILTHYGDPSYTVVSDYEIAYFPTIYRICPNRIV
                     DEVGQVSTAVLYASTDDCAVAFTNNDASIMDYTGPTVGCAEVELSITLQNMGFDPLTA
                     CTITANQDGVELLSYNWTGNLDIYEMEEVTIGSVVLDDESSNIDINITSADDNDLNNT
                     VYTTIFFHNNVNMVIHLEVKTDNYPTQTRWEIIDEESGDEVHSGGPYPTSDKNTVVVD
                     EDVTLPGNGCYTFHMFDSGGDGITGTGYYKIYDNGEVIADGDEDIAYKKSEALKVTGL
                     TEITAATVNQNLMISPNPANQSTSITVQHTGGDLFIELVDMLGQHIMTVNTSNCAAGE
                     QHYTINTSMLPEGNYIIRVQAIGNTQGSILTVTH"
BASE COUNT         5458 a         3852 c         3523 g         5178 t
ORIGIN      
        1 atcccattcg ccgcatgcag gactttgtgc aggattgcat ttgagcgtat atttcatgag
       61 tatcttttct acgggcacat ccaaacccgg cagatcaaac caggcatcct gaggagatcc
      121 aaaggtgaag gtttgaactt tgatggtatc tcccggatca gcctttacag ctgaaacaag
      181 tatcatcatc agaaaaattg tgcatagcgc cgttttcatg tatcgcaaca tggttgtaag
      241 tttggcacaa atataggaaa gcattgacgc attagtcggc gctgaattgt cataactcaa
      301 tcatacaaac ttggtaacag ccaccggtat ttataaatct tttggccctt tacaggtcct
      361 gaagggtgta gatttgcagg taaaccctgc agaagtagtt agtattgtag gtgcttcggg
      421 agcgggtaaa agcacgctgt tacacattat tggcactctc gacaaaccgg ataaaggcga
      481 tgtaatattg aacgatgtct cagtcaagtc actctccgaa aagcaacttt cagcattcag
      541 aaataagctc ataggctttg tatttcaatt tcaccacctg cttcctgaat ttaccgcctt
      601 ggaaaatgtg tgtatccccg gatatattgc aggcagaaag gaatcagaag ttgaagccag
      661 agcgaaggaa ctgctcgaat acttgcgcgt tagcgacagg atgcaacaca aacccaatca
      721 aatgtcgggc ggtgaacaac aacgtgtagc cgttgcaaga gccttgatca atcaacctgc
      781 tgtaatactt gctgatgagc cttcaggtaa tttagacaca caaacagcaa aagaattaca
      841 cgaattgttt ttccagttgc gcgatacctt caaacaaaca tttatcatcg taacacataa
      901 taccgagttg gcagatatgg ccgacaggaa actggtgatg caagatggac aaatggtatt
      961 gggctgaggt aagctgatct catttttttc tgaaaagtac tatcctgttc gaattcgtac
     1021 catgcgtatt attgcgaatt ccccaaatat tggctgtttt ggtaaattgt tgctcattaa
     1081 tcatcaatct atcaacttca aaaccggcat gtaatcccat ttcaagcaca atggtttcca
     1141 gacgtgtttt cccgtaatga atttcacctg tttcgaaagc cacgtgtcct cctttggcag
     1201 taatgcgata taattcgaag aatacttgtt gcattttctg catccatgca tcaactgttt
     1261 tcgacatggt gatgcgtttg gcaatttcat ttacatcgat atcgttaaac caacagcgca
     1321 gccaattgtc tttggcatat tgcactacat cgagaaatgg cggtgaggta acaacaagtg
     1381 taacggaatt gttttttatt tgtcgtgttg ctgcagcatc actattgatt aaaagagctg
     1441 atgttgctgc ctgagaaagg tatttttttg ttgaaatatc caggtctttt atcaggttgc
     1501 gcgacttttt catgattagt gcatgcacat cgcgatattc cggcttttgg ttgcgttttt
     1561 tattgatgag caactgcctt tcgcgcgata ctgcctgatt tggaggcaat gtataaacag
     1621 aaaaaaatcc gggcgaatga cctgtaagtc tgtttgtggc aaccatgcga atccatcggt
     1681 cgagagcgtc ttcagtgccc gaaagtttgc gcttttttaa ccactcccgc aatagcagaa
     1741 tttcatttaa tgtatgctta tgataaaaca tactaagtgt atgttgttct tttacaggag
     1801 ttcccgataa aatatgactt aactgctggt ccagagcctg taattccgga atacataatc
     1861 tcccacctga taatatggca ctaatgggat ttacatcatt ggcaataatt cgccttccac
     1921 gcagggcagc ttcaattacc gttgtacctc ttccgctgaa tggatcataa accacatcac
     1981 cttcattggt atatttttca ataaaatatt taggcaacga tggcttaaag caggctctgt
     2041 aggctatttc atgcaggtta tgaccctccc tttgtttaga agtccaaaaa gagccggtat
     2101 atacaggaac ccgtttccct ttgaattgaa tggatgatct gtttatacct gcagcttgca
     2161 tgagccgtaa aattcaccaa aaacagtgaa tatacatgaa catatacact gtggactgtc
     2221 ctgtatttgg ttgtaataaa aaaaactgcc agttttgcgt catcaaccat tgaccatgca
     2281 aaatacactt cctttcggta atgtcggtga cattacattt acaccttcgg gacaagacct
     2341 taaaatttat gtcagcggac agttgctagg aacttttaat tctagcagtc ctgcaaccgt
     2401 gtttattaca caaaatggca gcaacaaggc aatcaccttc aaacagattt cgcagagtgc
     2461 ttatatgcag ggtaacaccg gcactccggg agtagatacc atttatttca acccattaac
     2521 aggaaattca aaaatagaag cctgctcagg tggtgcggga tgttttaagc tgacgggaat
     2581 cggatgggag gatgacgact acggacaaac atgtacagga ggcagcaaag gcttcacaaa
     2641 cctgtttaac atttcagcgg aaggtcaact caaagctaag ttcgagcagg tatccataaa
     2701 cggaagcatg caaattcgat tggtagccag atacagcggt aacgttatgt gcaaacaaac
     2761 acctgctaca gccatatgct gtaatgataa ctactgttat tattgtaatg agtaaatgga
     2821 aatattaatt aaatggttga agcacaagtt cagccatttt tttgtcaccg gttttagaag
     2881 cacaccacga caaagtttct gtcatgaaaa caaagcgctt gtttccggat tcctttttca
     2941 gcactaacaa atcttcgtaa agcttaccat atagcggctt gcgttgtctt tccggagtat
     3001 tggcgatttt tttcagctga gtagtaaata gtcgctcgaa gtcatcgaac tttccgcgat
     3061 ttttcatgct gcggacaaca cttctgtaag ctgattcaaa gaacacaagc tgctcttgtt
     3121 caatcaatat gatcagataa agcatggaag cgccatgttg taattcaatt ttcacatcgg
     3181 ttttaaattg catgacgcgc tgacagtaat ccagggatct gtcgtactcg ccattgataa
     3241 aaaacatcaa cgaaaggtta tatggcgtaa caacaaggaa ggattctgta ataatatttt
     3301 caaacttgcg gcaataattc tcaaattctt ctgccagcat accacttcgg ctgtacatac
     3361 cgcgattcat ggcatagaga atcttattat tgataaccac ctgcgaaatg gaattatcat
     3421 ctttcactgc ttcacgaggc aattcttcaa gctttgctat cacctcttca aattcatcat
     3481 aattacctac aacatgcgcg gccgacaaat aattagaaag cgcgtcaata taatttccca
     3541 ttacattgag cctgtgttgc ggatagtcgc tgaatagatc tactaattgc tttgtcagct
     3601 gaaaagtgcc ttgatagtcg tggttatatt tacaaataag ccccttcgcc ctcagccaaa
     3661 agaatttttc tgtaaacgta tgcaggtctt caaatttttg attcaatact gcatcacgtg
     3721 atgaagtata ttgttcaagg tcttcattac tgcgcatcaa tggatctttg atgaactgaa
     3781 cccataaagt gaagaacact tcaccaagct tagattcatt atttaggcga tcaaataccc
     3841 attcctcttc agcatgtaat tttcggatcg tttcattcgg atcgccaccg gaaaatttca
     3901 aggcgtagat gcgttcgcgt tgcaaaatgg taaccaggca atagatcaaa tcatattcat
     3961 aggcgagttc tttggctttt tttaaagctt ttgatcgcat ttgtgtcaat cccttctctg
     4021 tataaaaaag ctcatcctga atgagattaa acacctctgt ggctacattt ttatcactgc
     4081 gatacaaccg catactctgc atgatcaggt catacaagta attttttgtt tgtggaagtt
     4141 gctttatcgc ggcagcagtc ttcaatttct ttttcacgat ttcttcatca tatccatcct
     4201 cttgagccat gataagatcg aataccttca tgtacagttt gtccttttca ttgccatgac
     4261 gttgcgcaaa aagtcggaaa taacgctttt catttgctga aagggcatga atcaggtggt
     4321 ataggtcttc tgccggacta tgcatgggct tgcttcaggg gtaaatatag gttgaagaac
     4381 tccagtttga aaactaactg ctcaaagtca atgccaaaaa ccgcttatta agcctgaaat
     4441 gatagcaata atcggttaat ttaccatttt tcatatccaa aaacggataa ttcacggttt
     4501 tccgttaacg gaactgataa ccgctaaaac gctatcccca attgcattac aggcgttata
     4561 catattaatt attttcatat ttgtctgtca gacgttgaag aatcggtcaa aatttggctt
     4621 gagcaggctc aggggtggaa atacctttgg cttatcaaac aacaaacact atgcacgcgc
     4681 cgatcttatt taccaaaaaa cgtcttctcg acttcagcaa acgcgaacgt cagatcttac
     4741 aattaattgc taaaggaatg acaaccagag agatagcact ggaattgagc atctccgaaa
     4801 atacagttga aagtcaccga aagcgcatga tccgtcgcgt ggaagcccgc aacatattcg
     4861 gtgtatttca ttacaccatc agcaatggca ttttacagcc ctcagttttc agtgaccctt
     4921 atttattgta aacacaagca caactctgct atcccaagtt tctccctctt caacataggc
     4981 tgttgaatcg ctgaccgtcc ccacccccgg gggcggtttt tttatttact tacaggtatc
     5041 tttgtccgac gatggaaata tttgcctaca tattctatat cggtgtgatc aatatcgttt
     5101 ttaccagcgg ttggaaactc ctggccacca tggtaggatc cctgcttaaa gatgtaggtg
     5161 tcaataagga taatactttc ctggtattta aaactgtagg ttattacata ttggtaagcc
     5221 ttgcagcact ggtaactgct cagcacatgg aaacaaccta caaacttgct gccatggcat
     5281 atgcatttac aggcactttt gtcatttatg caaccattgc cagtaatctg gagcgcaaca
     5341 gatggcgagc cgtcatgaat tttgaacgca aacgtattca agtaatgcgc tatgatggtt
     5401 atttgctgat ggcatgtatc gtgttattta tagcgactat attacaaccg caaatcgccg
     5461 accttgctcc acaccgctgg ctactggaaa ctattgatag tatatataat actattttct
     5521 taaaatgggt gataggtttc tttgcctttt tctacatgct gaatatcatt ttcagaggca
     5581 taaaattctc tgacgaagtg tttgccatgc ttttcaacag aaaacgcccg ggatccgata
     5641 ataatgataa ttccggcaca gaatatgccg attatgaaga gatcattgac gacgacagcc
     5701 gtttgccaaa ataatgccac ttcagaattc gtttatctga atattatatg cgccaaatcc
     5761 tgaccatttt tctattgtgc ctttcacaat tgctttttgc gcaggacgtt cagtgggctt
     5821 cggaagtagt gaaattttca actgaataca gccgaacaca attttctgca aaacaagccc
     5881 ttggcgctcc aaataaactt cctgcctccg gcgaatctac tgttgcctgg gctccatcta
     5941 ctcctgataa tccggcagga gaatttattc aagtgcgctt tgctcagcct caatatgtgc
     6001 aacaaattgg tataggcgaa agcaatgccc ccggcgctgt taaagaggta gtgcttattt
     6061 ctaccgaagg gaaaaaatat accgtctaca ccaacgatac catcaagccg gcttacaata
     6121 caggaagtcg cgttttcaga atctttatcc cacgaactga ttacctggtg aaagaagtaa
     6181 aagtatcact caatacaaaa gtgatagcag gtatgaatca aattgattgt attggtattt
     6241 ccgatagccg cgaacctatc aaagccaaaa taaatgaact cgcttatgcc gagaaactgc
     6301 ctccaccgga aaaccttggc actaaggtaa attccgatgc ggatgatatg ttcccattta
     6361 tttctcccga tggcaaaacg ctctatttta cacgcaaaaa ttattttgaa aatacaggcc
     6421 ctgacctgcg cgacgatatc tatgtatcca aattagataa tgacaaatgg acaaccgcca
     6481 taaatatcgg ccctccatta aataatgaac aacacaattt cgtatcgtgg atttcacctg
     6541 atggaagctc attgttgctt gccaataact accaaaagat aaaacaagat atttccatat
     6601 ctaccaaaac cggcaccgga tgggcatttc caaagacatt aaagatcaac gatttttaca
     6661 ataataacga gttctcatgc taccacatga ataccgaagg caatgtgatt ctcatggcaa
     6721 ttgaacgccc cgatacacac ggcgatatgg atatttatgt aagctttttg caagcgaata
     6781 aagtgtggac cgaacctaaa aatattggtg gaactattaa tactgccgct acagagggaa
     6841 gtatttttat tgccgcagat aatagaacta tctattttgc ttccaatggt cattccggct
     6901 acggaggata tgacatgttt atgagcaagc gcctcgacga tacatggaca aactggagcg
     6961 aaccattaaa cctcgggtca caaataaact ctacgggcaa cgattattat ttcactattc
     7021 ctgccagtgg cgattatgta tatttttcgt caaccaacaa cagttatgga aaagctgatt
     7081 tatatcgcat cccccttcca aaaccattgc agcccgaccc tgttacattg ttgaaaggaa
     7141 ccatcatcga tgccgagacc aaaaaaccta ttcctgcgga tattaatttc ggaggattag
     7201 taagtgaaga accggatggt atttcaacta gtggcgatgg cggctatcaa gtggtagttc
     7261 ctgaaagcaa ttatctcatc agtatcatca aaccgggcta tctcccctac tctgcaacaa
     7321 ttgaccccga gttgcattac gatgccatag attataatcc ggaagatagc atcgctacca
     7381 ttaaacacga aatcactacc acactaaaag cagaaattaa aaccaatccc ataacccctg
     7441 aagcccttga ggaagaagta ataaaattgc tccccgatga aattgcagat acttcatcta
     7501 cagtccgcga ttctattatt cgcgatatta cagatgcttt tgaaacgaaa gacagcacct
     7561 ataaggaagt gacagctgac attgaaatga ttcctatccg ggaaggaaca gtaattcgac
     7621 tgaataacat ttatttcgaa gcaaacaaaa gcgatttgag accggaatct tcttcagcat
     7681 tagatgaact ggttaccttc ctgaaaacaa atccaaacat ttacgtcgaa attggcggac
     7741 ataccaatgg attaccaagt gatggtttct gtcaaaaatt atctaacgac cgagcaaata
     7801 aagtggttga atacctcatt gcaaatggca ttcctgccga tcacctaaca tggaagggtt
     7861 atggcaaaac acaaccaatt gccgacaatg ccacagttac cggacgccgt caaaatcaac
     7921 gtgttgagct gaaagtgatg aaagtggaat agtgtcggtt ttctttgcca aatttgtggg
     7981 aaatttagca catgtcgtca ttgagtttcc atgtattcat ggacaatccg catacccaca
     8041 cgtttaccat taagatgcac atacctgcaa tgggaaagga tgtactttcg cttgcattac
     8101 ctgtttggac gccgggctcc tatttggttc gcgaatattc caaaaacatc aatagactat
     8161 attgtgaaga taaaaacgta ggcattaaca agactgcgaa aaacacatgg gagatcacta
     8221 ccaataatca ggcgatagat attatttatg aattatacgc cttcgaagaa agtgtgcgca
     8281 cctgttggtt agatagtgaa cgtgcttcca ttattcccgc gggtatgttt ttattcattg
     8341 aagacacccc gcttccggtc aacataagtt ttcatccaca tcatgcattt aaagatatag
     8401 ctacttcatt acctatggta aacaatgatc catgggtgag atttgctgcc gatgctgata
     8461 cattatatga tagtcccatt gaaatcggaa accatataac acaacatttt aatgctgccg
     8521 gagttgagca tacattggtg atttccggca ccgggaatta tcatattcct tccatgataa
     8581 ccgatatgca ggctgttatt gaaaccgaag taaatatatt taaacatcat ccttgcaaat
     8641 cgtatctcat catcctcaat accagcaaca cccttcgtgg aggacttgaa catttacaca
     8701 gcaccagttt aatttttaag cgattgatgt ttgccgatcg aagtaattat caggaattca
     8761 tttcattatt tgcacatgaa tatttccacc tctggaatgt aaagcgcctt cgcccaaagg
     8821 cattagggcc ctttaattat attgccgaaa actacaccac ttcgctttgg attgccgaag
     8881 ggttcacaag ctattatgat gatttaatag tatatcgaac cgggttgtat gcagaagagg
     8941 aatatcttac aatagttgaa aaaaatatca atctggcaga aaatgccccc ggtaaaaaaa
     9001 tcatgtcggt tgcagaagca agttttgacg catggataaa atattatcgc caaaatgaaa
     9061 atagtgaaaa cagtcaggta aattattatg tgcgaggagg tgtattggca acattactgg
     9121 atatactcat catccaccac tcagacggca agcacaatct cgacaatgta atgcgcactg
     9181 cctatgagcg tttttatgta aatcgcgatt ccggatatac agaagatgaa ttcaaagctg
     9241 tgatagaaga attcgccggt atatcaatga atgactttta tcgtcaatat gtattcggca
     9301 cttcacaaat tgcattcaac gatatattat gtcatgtagg acttcaatta gaaaacacac
     9361 tggaaaatac tattgaatca gatcttggca ttaccataaa taacaataac atcatcaccg
     9421 ccgtgcgtcg caattcagca ggtgaacaag ccggaattta cgtgtcagat gaaatcattg
     9481 ccataaatgg ttatcgctat agcaatggat tattggctag tttaactgca gatgcaaaaa
     9541 ccggtgataa gctggagata atattgtcta agtccggacg catgcacaca gtttcggcga
     9601 ccatacaaat aacagacaag gtgaattatc gcttgaaagc aatggatgat gcaaatgatt
     9661 cacagcgtgc acactatgcg aaatggttgt taaagaataa ataaaaaaca tggaaactct
     9721 gcataccgaa cgattaacat tatcaccctg caccattgat gatgcagcat ttttattagc
     9781 attattaaat tcaccgggat ggttgaaata tattggcgat agaaatgtgc ataccctcga
     9841 agaagcgcgt tcctatatgc aaaaccgcat catgaaagcg tatgaaaagg atgattatgg
     9901 catgtggtta gtgcgcgata cgacaacagg cgctgctatg ggattgtgtg gaattgtggt
     9961 ccgcgacacc ctgccgcatc cggatctcgg atttgcattc ctacctgaat ttcagggaaa
    10021 aggttttgcc tttgaagcag ctcaggcagt aatggaattt gcaataggtt acaccgatat
    10081 tgataccatg cttgccatcg ccacccccga caatacgcat tccataaaat tattgacaaa
    10141 actcggcatg tcctttgaat ccgaattcac tgatccgcat accaaagatc tattggttcg
    10201 ttattctata aaccttaaca tttaactttc aatctacaac cctcaacctc ttggtggaat
    10261 cgagtatcta gaatcgagta tatagaacca agtatctagt atctagtatc tagtatctag
    10321 aaccaagtat ctagtatcta gtaatcccta tccttcaaat cttcgaatct tcaaaccttc
    10381 gaatcttcaa atcttcaatc cttcaaacct tcaaatcttc gaatcttcga accttcaccc
    10441 cctaccatca cccacgcact accgttctac tgtcgctttg tgcagttgga agtatagtaa
    10501 tctcctcaag attcatgtgc tgaggtcttg atgccgcaaa atagattaca tcagcaatat
    10561 cttttgcctg taaaggatca aatccttcat aaacggcttt agccttcgcc tcatcacctt
    10621 taaatcgcac catgctgaat tctgtttctg ccagtcccgg agagatattc gttaccttga
    10681 tatgatatgg caataagtcc atgcgcaagc ccttgctcaa tgccgtaact gcatgtttag
    10741 tagcacaata gacattcccc ataggataca cctcctgtcc tgcgaggctg gacacattga
    10801 taatatgccc gctttttcgc tctttcatgc cgcttactgc gtgtttggtg acatataata
    10861 atcccttcac attggtatct atcattgcct cccaatcgtc aactaaacca tcctgaaata
    10921 aatctcttcc cgcagcaaga ccggcgttat taatcaaaac atcaatttgc ttccatgagg
    10981 caggaatggt atccatggca gttgctacgg catccttatc acgtacatcg aaaggtaaca
    11041 acaatacatc ggcgctataa gtctccgaaa gcatgcgttt gagtgtttcc aatctttcta
    11101 tccgtcgcgc attcaaaatc aggcgatatc cgttttgagc aaaaatttct gctgtagcag
    11161 ccccaaaacc ggagcttgca cctgttatca tcactattgc catagcttta cttttttgca
    11221 agattaacaa aaaagccgtc actattcgtc gctcttttgt cgccactagt cgatacctga
    11281 accctgatcg tccaaacaat tttgccaaag ggcatgttat cccgttactt tgcataaaat
    11341 aacttagtat acctctccga aatcagatca atgaaacaag ctatactcct atttattggt
    11401 atttcttcaa taacggcatt aacggcacag accaccattt caggaactgt taccgataac
    11461 aaaaagaatc tgttgccggg agccaacatc tttctgaaag acacctacga cggaacaaca
    11521 agcaatgtgg atggcacata tagcttcatc accacagaaa caggcaaaca aatactctcg
    11581 gtaagctttg taggctatga aagttttgag gacacactcc aattaaacgg aggcacctta
    11641 tcggtgaata ttgtgctcaa ggaagcattt aatgaattga atgccgttgt gatcactgca
    11701 ggtgcatttg aagcaagcga tgaaaaaaag aataccattc tcaaaccgct cgatattgta
    11761 accaccgccg gtgccgaagg cgatatttac ggtgccctgg aaacattacc cggagccagt
    11821 aaggtgggca acgacgatgg tctgtacgta cgcggtggta gcgattacga aacaaaaacg
    11881 atcattgatg gcatggtggt gaataatcca tattacagca ctgtgccgga tgttcccagc
    11941 agaggtcgat ttcaaccctt catgttcaag ggcaccgttt tcagcacagg gggatattca
    12001 gcggaatacg gacaagcact ttcatctgct gtaattttaa atacagaaga catgccggaa
    12061 tacagtgcat caggattaag tgtcgcacca atatttttag gcggatttca cacacagaaa
    12121 tttaaaaatg aaaaaacagc attaggtggc ggtattaatt acaccaacct cggattattt
    12181 gataatgtgt tcaaaccaac tactttcgat tggataaata gtgttgaatc tgttgatggc
    12241 agcttattct ttcgtcagaa aacatctaaa acaggtatac tcaaactata tgcccaaaag
    12301 gaatcttcaa acatggcatt gcgtgcgccc gatatcgaca gtctgccggc taccgatgat
    12361 gtttatttag gaaataatta caattatatc aacgccagct ggcgagaaat cctcacaaaa
    12421 aaatggggtg tgcaggcagg tgtgagctac agtaaaaaca atgatgatat ccttgtagat
    12481 ggtttcaatg ttggacgctc cgatcaaaat gcgcagggaa aagtaacctt caataatgag
    12541 attaccgaaa aaattaaatt gcgctacggc ggcgaatttc aacatatcac ctacgatcaa
    12601 tttgccggtg atctggaatt tgatgctacc gacaatttca cggccgcatt caccgaaagc
    12661 gacattttta tcaccaacga ccttgcagga cgcattggtg ttcgagctga atattcggaa
    12721 atactggatg atgtaaatgt tgctccgcgt atatcattag catacaaaac aggaaaaaat
    12781 gcgcaggttt cacttgccta cggcgatttc tatcaaacgc cgcaacccaa ttacctgtat
    12841 acgcaggcgc tctatccatt gcaatatgaa aaagcaacac attatatcgc caattatcaa
    12901 tacatcacca atgatcgcac gttcagaata gaaggatatt ataaaaccta tgatcagttg
    12961 gttacactga caacggattc tctaaatcct ttcgtaccga ttaccgcaaa caacggatcg
    13021 ggatatgctc gtggcattga tttattctac agagataaaa aaactattga ttatgccgac
    13081 ttttggattt cctacagctt ccttgacact aagcgcaaat tcttggatta tccggaagaa
    13141 gtaacaccaa ctttcgctgc gcaacatatt cttagcttgg tgtacaaaca ttattttccg
    13201 ggcatcaata cacaagtgag cggcacctat cgctatattt ccggatggcc atattacaat
    13261 ccaaacaatc ccgaattttt gggcgatgta tctccggatc tgcataatgt tgcacttagc
    13321 gccagtgtat tgcagagcct ttggggtaat ttcactgtct tctttatttc gtgggataac
    13381 acctttggat ttgatcagat ctatggatat aactactccg ccgatggctc acaattcctt
    13441 gtaaatcagg ctcctgtgaa gagtacagtc tttttcggca tctttatgag cattttatac
    13501 ggaaaagata aaaacgtaga cgatatcgac atcgataagt cgaacaaatg ataacattca
    13561 aattcggaac actttttgta aataagcacc ttactatgaa aaaaatatta cttgtaacac
    13621 ttgtcgccct aacttcaata ggcgctcaag cacaaactgc taagtttcac gacggtatga
    13681 aaaaagcgct gacgcttatg gatagcgcca aaaccggaga tgattttatg aacgttgcca
    13741 atagctttga acgcattgcc atggcagaaa aaaatcaatg gctgccctat tactattcag
    13801 ccttggcacg aacaactgca ttattcattt ataacgatgt aacgcgtaac gatgctgtgc
    13861 tggatgttgc tgaaaaacat gccatgaaag cagattcttt atcaccaaac aactcagaaa
    13921 tatttgtcct tcgcagcatg atttttggtg gtagaataat ggttgatccg atgtcgcgcg
    13981 gacaaatgta tggcatgcag agcatgatgt tattgcaaca agccatgact gccgatccaa
    14041 ataatccaag atcatattac atcatgggac aaagcctgtt ttacacccca ccacaatttg
    14101 gcggcggtaa ggaacccggc tgtaaaatgt ttgaaacagc gttacagaaa tacagcatat
    14161 ttgttcccca aagcgatctt catccaaatt ggggattaga tcaggttaat caaatgctat
    14221 cacaatgttc tgctaccacc gatccaaata ctggtaagtc gggcggaaat tgatgatttg
    14281 aacttatatg taaaccgcta cactccggag cggtttattt ttactcattc ccagccgatt
    14341 ttaagccgaa attccatcag aaataaggct caaacactat tatgcattag catattgtgc
    14401 acaagtctta aatcttgtta ccatgtttta gggaaaaagt atgatcttga gatttatggt
    14461 ttggtcatgg aacattgaat ctgataacct tatgcaaccc ctaaccactg ctttcaatag
    14521 aattaaacct atcctattgt agtttctatc gaaggactag gtgaaataac atcgacatat
    14581 atgttttgat caatttttaa tcttatttta ttgattttct tataattaca atgattatat
    14641 aatataataa tttagataat tcatcataac atatttacat ttttataagt tttatttaaa
    14701 gatcaggtat agattccctc caaaatataa ctctgattca ttgtcattta tacaggcatt
    14761 tatagtctta gagtggatta ctgaaggttt acggtcacgg gattatcata aaatagatgt
    14821 ccacacagtt atttgggatt ttacgcaact tctggtatct ttggtaagta atattctaat
    14881 cagcgaaaaa tgaaaaaact tttcctctct ctttctctag ctgttgtggc aatcctcgga
    14941 ttcaacactt cagcaaaagc ccagttggaa gacggctctg tagcccctga ctggacactg
    15001 accgatatta acggtaccac ctggaacctc tatactctcc tggatgaggg ttacgatgta
    15061 ttccttgatt tctcggctgt ttggtgcgga ccatgttggg cataccacac cggtggaagt
    15121 cttgaaggct tatacgatgc ctacggtcct gatggcacta acgaggtaat ggtattttac
    15181 atcgaaggtg atggcgcttc tacattggat gaattgaatg gtatcggtgc cggaactcag
    15241 ggcgactggg ttacaggtac tccttatcct atcgttctga cacatgccgg agatccaagc
    15301 tattcagctg ttcaggatta tgatatcgct tacttcccaa ctatctaccg cgtttgccaa
    15361 aaccgtattg ttgatgaagt tggacagctt tcaactgcag cattgtatgc taccatttct
    15421 gattgtgctg ctgcttcagt taacgttgac ccaagcatcc tgacctacaa tggccctgtg
    15481 gcttcttgcg gcgatgttcc tgtttctgtt actatccaga acatgggctt caacgaactt
    15541 actgcttgca ccattactgc ttatgctgat ggcattgaag tagcttctgt tgattggaca
    15601 ggttcattga acacttatga aattgcaact gttgatgttg gtactttcac accagctgat
    15661 gctgatgttg acctttcagt tgaaattaca tctgctgatg aaaataccgt aaacaacgtt
    15721 atcgctacaa ccgttactta cgaagacaat atcactcaga tcattcacct cgaattgaaa
    15781 accgatagct accctactga aacaaaatgg gaagttatcg acgaatctac aggtgatgta
    15841 gttgctgaag gtggtcctta taccaatgca caaaaaaatg agatcgtgtt cgatgaagac
    15901 atcgtattac cttctcttgg ctgctttaca tttacatgct atgatgctta tggtgatggt
    15961 atcacaggtt caggatacta caaattgtat gataataacg gtgaccttat ccagcaaggc
    16021 atggaaaaca ttggctttga aaaatctgct gctttgaaag taactactgc agttgctatt
    16081 gaagaaaaca gcgctataaa caatgtttcg ctttatccta atcctgctaa cgacaatatc
    16141 aatgtagaca tcaacctcac agctactgct gatgtagatg ttactattgt taacatgtat
    16201 ggtgctgttg taggaactgc tacttcaggc acacttactg caggtgctta tagctttact
    16261 gttaacaccg ctgatcttgc aaacggaatg tatttcgttc aaatcactgg tgaaaacgta
    16321 aacaatactg tgaaatttac tgtgttccat taattaggaa ttccctagat attgagctcc
    16381 cgtaccttcc gtacgggagc ttttttattt aaacgaagct gtctttcgtg aatcgtgaat
    16441 cgtgaatcgt gattcgtgac tcacccactc accactcacc actcaccact caccatcatc
    16501 cgtttccgat aaatttccta agtttgatat ccaaaaccct gatcatgaaa aaaattacct
    16561 acccctgtat attgcttatc atcagcctga tgatggttgc tccggccaaa gcacagctgg
    16621 aagacggatc tatagctgaa gactggacgc tcaccgatat caatggcacg caatggcatt
    16681 tatacgatat cctgaaccaa ggcaaaagcg ttttcctcga tttctctgca gtttggtgcg
    16741 gaccttgctg gacataccac aactcgggta acctggaagc cttatacgac acatacggac
    16801 ctgatggcac caatgaggta atggttttct atatcgaagg tgatggagcc tcaactctcg
    16861 atgagttgaa tggcatcggc gccggaacac aaggcgattg ggttgatggc acgccctacc
    16921 ctattatttt aacgcattat ggtgatccaa gttataccgt ggtaagcgat tatgaaatag
    16981 cctattttcc aacaatatat cgcatttgtc ctaatcgcat agttgatgag gtaggccagg
    17041 tatcaacggc tgttctatat gcaagcactg atgattgtgc cgttgcattc accaacaacg
    17101 atgcaagcat tatggattat accggtccta cggtaggttg tgcggaagtt gaattgagta
    17161 ttactttaca aaatatggga tttgacccac ttactgcatg cacaattact gccaatcaag
    17221 acggcgttga attgctgagt tataactgga caggcaattt agatatttat gaaatggagg
    17281 aagtgaccat tggctctgtt gtattggatg atgaatcatc aaacatagac atcaacataa
    17341 cttctgccga tgacaatgac ttaaataata ccgtatacac cactatcttt ttccataata
    17401 atgtgaatat ggtgatacat cttgaagtga aaacagataa ttatccgaca caaacacgct
    17461 gggaaattat tgatgaagaa tccggcgatg aagtgcattc aggcggacct tatcctacca
    17521 gcgataaaaa cacagtggtt gtagacgaag atgtcacctt gcctggcaac ggttgctata
    17581 ctttccatat gttcgattca ggtggtgatg gtatcacagg aaccggttat tacaaaatat
    17641 atgacaatgg tgaagtaata gccgatggcg atgaagatat tgcatataaa aaatctgaag
    17701 cgcttaaagt gaccggactg acggaaataa ctgctgcaac cgttaaccaa aatttgatga
    17761 tatcgcctaa tcctgcgaat caatctactt caattaccgt tcaacacacc ggaggcgatt
    17821 tatttataga gttagttgat atgttagggc aacatattat gaccgtgaat acatctaatt
    17881 gtgcagccgg tgagcagcat tatactatca atacctcaat gttgcctgaa ggaaattata
    17941 ttattcgtgt tcaggcaatc ggaaatacgc aaggaagtat acttaccgta acgcattgac
    18001 caaacaattg t
//
LOCUS       DAOQHY010000056        18009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1445_length_18009_cov_5.061045, whole genome shotgun sequence.
ACCESSION   DAOQHY010000056 DAOQHY010000000
VERSION     DAOQHY010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 18009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18009
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1445_length_18009_cov_5.061045"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PLL28_06315"
     CDS             <1..409
                     /locus_tag="PLL28_06315"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNF68971.1"
                     /translation="ERDLLYKVLFDMKQDLNDLKQFVLHMAEANHLKTDMSISAEMHD
                     QPTFNELSTRQPQLQTEFITKQPVVINPANSAAHAHVHEEVEESLSLIDKEKELINKA
                     LSKHRGKRKDAAMDLGISERTLYRKIKEYNLDE"
     gene            402..914
                     /locus_tag="PLL28_06320"
     CDS             402..914
                     /locus_tag="PLL28_06320"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HNF68972.1"
                     /translation="MNKRRITYSLVCVAFLLSIAATCKVSFNGAQIPVDVKTLTINYF
                     PNQATLVSPSLSQTFTESLKDKFLRESSLTLTEQGGDWELSGAIVKYESTPIAPTGQE
                     TTALNRLSITVKVDFTSRKSEDADWSQNFMRYEDYESAKTLSQVEGDLLPKLSEQLAD
                     DIYQKLTQNW"
     gene            914..2017
                     /locus_tag="PLL28_06325"
     CDS             914..2017
                     /locus_tag="PLL28_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68973.1"
                     /translation="MFTEEFNSIITNPHSLQDVPLADLKVLAERYPWCQPVQVLYARK
                     LFLVESPQFEHQLHRTAILSYDREVLFKYINAEIPEVISVAPEPEVQPDQPVIVEEHQ
                     EIQPVVEISEPELDQISVEEFDPATEELIQEMIESIPEVHQEDEIAPIEMMSEETMEL
                     QEEEIEAESMLQEEMDLENLDHAVINDDMEAVASNPLPDERIEPDEAFIFEMPAYDIE
                     TELGTLDEADKFTLPNIPPEVAAEKNTEVFTDSFTGWLNRLGGSPTGKVVEQKASNAP
                     VKVYLRKQPTPGSDPAGSAHERLMNEQYAAELARKSIQSDDGLISETYARILVMQGKY
                     QRASEMYRKLSLLKPQKSDYFAALIDQINKKIK"
     gene            2019..2372
                     /gene="secG"
                     /locus_tag="PLL28_06330"
     CDS             2019..2372
                     /gene="secG"
                     /locus_tag="PLL28_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNF68974.1"
                     /translation="MLFTVIIIIAIIAAVLLGLVVLIQNPKGGGLSGTFGGAANQIFG
                     YKRTTDDIEKITWILVAVVFVLCLSASALKPVETVTQQGGGLGTEAKMPTNVAAPAPD
                     LMDEQSADQPGDTAN"
     gene            complement(2454..3008)
                     /locus_tag="PLL28_06335"
     CDS             complement(2454..3008)
                     /locus_tag="PLL28_06335"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HNF68975.1"
                     /translation="MRNSILLAIALIIQTAVHAQIGSPFKGNEEADTIYFVELDSFTI
                     TARPLKNYNYNKYEAIVKRVYPYADTAVMTLKALQSQSFSGHHDEKQYKKELEDQLRK
                     TFEAQLKKLTRTQGEVLIDIIERNTGSSMYNILKETKGATSAFWWNNLGKMYGYDLKD
                     SYKPTNNPTLEEIIVQYEKKYKGK"
     gene            complement(3044..4192)
                     /locus_tag="PLL28_06340"
     CDS             complement(3044..4192)
                     /locus_tag="PLL28_06340"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNF68976.1"
                     /translation="MFSSLIGALLTNGLALSMGCSNGESSIPVKPVESPDTLVTFTIA
                     AVGDLMCHSTQFNYAKTGVDTFDFTPSFSLVAPILQQADLAIGNLETTLAGKARPYSG
                     YPMFNTPDQYVRAVKQAGFDFLVTANNHSNDSGDKGIVRTISILDSIGLGHTGSFASA
                     NDRDSIRILDIKGVKIGIISYTFSTNGLPLADGKPWLVNMCDSALIKKDMAAGRAKGA
                     ELMIVFYHFGEEYQRLPNAYQKQFVQHAIDCGADVVLGSHPHVIQPMEVVAGRRSNVD
                     SVFVAYSMGNFISNQKDQYTDEGVIMQLTFEKHLPSGKIRLAAHNNYVPTWVYRGVAD
                     QLKMHIIVPVSAAPGNDTKAIYLNDAYQSSISEAKKHTDAILHTVVPK"
     gene            complement(4220..4417)
                     /locus_tag="PLL28_06345"
     CDS             complement(4220..4417)
                     /locus_tag="PLL28_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68977.1"
                     /translation="MLLKQTFRPLRYTTLLFLLYVANISLKSQSKGNGSMTQFYIVTI
                     VFTVLILAAVISIFISKKKKK"
     gene            complement(4571..6103)
                     /locus_tag="PLL28_06350"
     CDS             complement(4571..6103)
                     /locus_tag="PLL28_06350"
                     /inference="COORDINATES: protein motif:HMM:NF019351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HNF68978.1"
                     /translation="MNRRSFLRNTLAGTTAIAVTPAAFLASCSDGNQDILDDVLFGTV
                     GGTTVPTPPFVSPFNTLDTQVANGSTITANRNFYTFAGISGTFCYGYSESVWGPTLTA
                     NTGDNISLTLESNLSSFGTNLHFHGLTLDASQEDGVENGVASGASKSYSFTVNNRAGM
                     YWYHAQYQKLGGDQLGLGLGGLFIVSDSEEDALNLPNGNLRSTTISLQDKRFNEDNQM
                     LYGPNKAEYLTGYFGGTILVNGRIGVFKNVSSRFHRLRIINGSTARIYNLSISTTDGT
                     ELDFNIIGNDGGLLKSLGGGIKQVLLAPGERLDSIIDFSTLSVGTEVLLSSNQFNAAG
                     DYQGVTGFDIMKFVIDTADDETWSLPDDLSDITTLTEGDVTGETRNFDIANANISKDE
                     ATNNTDNMHTINGDAYDAGTVNFTVNAGTTEKWVLDNTGGSEPRVMHIYGVQFQVLDR
                     TGGRGSVKQWEKGWKDTILLLPDEVVTLLVPFTTAPGVYYLASTNQEHADTGLINRFE
                     IV"
     gene            complement(6260..7186)
                     /locus_tag="PLL28_06355"
     CDS             complement(6260..7186)
                     /locus_tag="PLL28_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68979.1"
                     /translation="MPSKKILIPVIAVLPWISACNKDVTIFADVHFSFAFDSTQTRLD
                     NLGEPSEVPAGHGSQSPETQLMSIHYIELTTGSNVQLGDGAVLYKAPETLAGGAQAIL
                     FDSSILASEGSDFLQIKLERLAPGTYTYLRASVAYQQFGISYDLHNVPIYGDVLDQDG
                     VLASFLGYNTYINDLHIQNLTTLVNANKEQGFWGFETLFSGDLSAYNAIYTGQAPEGA
                     TTVVNPINATSPIPAGSCVVTGMFAEPLVITGDEVQDLYITFSFSTNNSFEWIDSDTD
                     GKWDIDVLDAGATESVVDMGIRGMIPSWEWRD"
     gene            complement(7292..9568)
                     /locus_tag="PLL28_06360"
     CDS             complement(7292..9568)
                     /locus_tag="PLL28_06360"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNF68980.1"
                     /translation="MVSNSKLWKWVAGLKPVYVIAVFSLLVLLAVFFTLQLRFSYDYE
                     DFFPENDPDLAFYDAFRQQFEPDDNFLLVGMQPENGLFDAKFLTSLDSATNAVGRIEG
                     AERAYSVTNFRYFVKLPFGFIDYPAVHPDDSSRFESDSLRLAADVRVVGKLVSEDFST
                     VVILIKTNDSLSQQKAKSFVADVRAAMHDAGIKDYHLLGKANFEVELVEIQKKEFLIY
                     AMLSIVLVTIVTFLMFRKIWCVVLSVLTVAISLILFTGILGGLRIEQNVMSTLYPIVI
                     IIIGISDAVHFIGKYITELRKNNNRKVALFRTLSDIGFATWLTAVTSAIGFLTMLTSN
                     VPPIKDFGLYSALGVLVVYAVVLFFISPVLTLFRLDQLDAKTDGEDKKWTAFINAIYT
                     LGIRRKRRILIIMSAAMLIFIYGVTQISTNIHLEAGMPKGAQVTTDFHFFEEHFNGFR
                     PLEIAAEAKGKHLITEPAIIRDVEKVETYASSFDLVNGLQGMTMPYKSMHRAYNGDIA
                     SYYRLPENDSILYGYQKLIDKYATREMHALISSDKKAGRISGFLSDVGTDSIRMFQTD
                     LKKFIEEKTDTSAVKFTITGTGVIFDKNTQYLRKNIISGVLLAFLCIGIVMAYLFRDW
                     RMVIISIIPNILPLIVCAGIMGLLGIELDAPTSIIFGISYGIAVDDTIHFLSKFRIER
                     QKGYPLEIALQNTFQETGKAVFTMSAILFFGFMILMLSSTSVTFNIGLLTGVTLFTAI
                     WPDVFLIPILLRMMKTKL"
     gene            9753..10142
                     /locus_tag="PLL28_06365"
     CDS             9753..10142
                     /locus_tag="PLL28_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68981.1"
                     /translation="MKQLLQKLTFSLLLVSAVTFMASAQTPDRGNATIDANMVIHLDE
                     TQPLVADYSFSFSAIAFKNEAAATRFFGLCRDNIVSYNLDWAAKTATVHIALEYMEPR
                     GWDIVKYNDYFSKLAERYRTTLSVVNE"
     gene            10175..12361
                     /locus_tag="PLL28_06370"
     CDS             10175..12361
                     /locus_tag="PLL28_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNF68982.1"
                     /translation="MQSRIIAVVAMVVFSLFTINSATAQIVGANVFLKGNYVEVGINT
                     CGAYGTPSAPPAGYHPTEGGLGFVADWESDGWGSGTPVYCGDYFVPGSPVEGWQLQIG
                     PTWWTNTDQSCGGSDIPGDVSDYTYAGGVYTGVWTGNLTTGTTDLDITQITTLPEDAL
                     YFVTRILLCNNGADPLNNVYYKRNVDPDNDQPWSGDFTTDNVIVYQPPTDEEALVTSE
                     GLTYGCFLGIGARDENARVSYGNFGTDDGSPKAVWDATGGYVGTGSEVGDIANSISFY
                     IPVINPGECKCVAFAYILNVDDLEAALEATVTYNLTADDITVASGDTVRTCNPGDSVE
                     LSILGADDYFWTWTPTTGLSTDTGISVIATPDVTTTYTITGVGGFCGDATLQVTVEID
                     THEFADAGDDVEICLGDVTTLTGDGGPYENIYAWTPAADLGCADCATTTATPTTDGVF
                     TYTLTTYDIYNCPATDEVEVTVHPLPDVNAGPDKDLCPEGAVDLEATGAIEYTWSPSD
                     GLSCDDCPDPTCTVDENTTYTVTGTDANGCVATDVINVNVFPNLEGIMTVDPTEITID
                     VYQNETAQLNAYGAEIYQWSPPMGLNNVNISNPIAQPADTTVYIVTGVDANGCTDVDT
                     VTVNVIGSLEIGVPTAFSPNGDGTNDFWFPMISGSGCVESWTVFNRYGEVVFEGNGFC
                     PGEVTQGWDGTYNGELQGIGTYVVIIKAATTLLEDRFVQGNFTLVR"
     gene            12467..14383
                     /locus_tag="PLL28_06375"
     CDS             12467..14383
                     /locus_tag="PLL28_06375"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNF68983.1"
                     /translation="MKHLHRLLPLLILLTCTQMQAQIVGANAFLKGNFVEVGINTCGA
                     YGSNGAPPAGYHPNVFSGLGFVADSDMDGWTTGTPDYCGDYFVPGSPVEGWQVQIGGT
                     VYTNTDQYCWTSEIPGDITSYDYTAGIYTGIWEGTIAAEDVTITQTTTLPENARYFVT
                     RILICNDGPTDLTDVYYMRNVDPDQDQPFGFSFMTTNSVIFQPPVDPDALVESVGDVY
                     GCYLGMGARDENARVTYGCFSTTDGTPAQAWSGASVCAWSSGYSLSGTYYCDCATQVT
                     FKIPLIAAGECQCVAFAYILDADDLDEALEATSSIGVAADGVDISTSGITLICPGDSV
                     ELNIIGGEDYDWTWTPTTGLSSGTGDTVVASPDITTTYLATGVGICGTIEREVTVEVP
                     PPPIANAGPDMSLCPSDTIHLAGSGGVHYQWTPPVYLDDDTLSNPAVMDPLTDMYYQL
                     VIWDANGCTDTDDVMVTLYDLPIVDAGDDQYMAEGGFAQLTASGAATYVWTPEDFLTN
                     ALIYNPIAQPEDTTMYYVVGTDANGCENIDSMIVYVLDQTHIVVPNAFTPNGDGLNDT
                     WKPVLIGIGEITYFSVYNRWGDLLFESSDPTIGWDGTYQADEQEIGNYIVMVKGLDGF
                     GQPLTKSAMVLLMR"
     gene            complement(14535..15425)
                     /locus_tag="PLL28_06380"
     CDS             complement(14535..15425)
                     /locus_tag="PLL28_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68984.1"
                     /translation="MRIRPVLMIIFVSLCVVHSYAQPLQIINSTTKPLLTNYYDAVER
                     ADTLKALHALNGLINEDPDFPYYYIARASIHIALGNIPQDSILIDYERAIERAPDDIT
                     VLSQQAVYTGQFHSDNMRIIAMNDRLSLVQLDSLNYNHYEACNNYVLSKDEALIAAFY
                     QQATRAMFQMVTMYPDSSDTWYHLANVISWNYAFNGNSEMLLKALSYYDKAIQLNPAN
                     TVFYIARATLFAKSKDWENAIEDYKQAMSLSSRADWFLELGLLYEHIGEKHKAKNAFN
                     TGLQFYPDNYLLRRAKKQLH"
     gene            15554..17614
                     /locus_tag="PLL28_06385"
     CDS             15554..17614
                     /locus_tag="PLL28_06385"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HNF68985.1"
                     /translation="MLLSCLSTSMLHAQSLLTCGTDIERSKLIADHPEILDREAIFDA
                     LAAKKFAEVARIEGDVLVIPVVFHVVHDYGPENISDAQIFDAIEHINADFSATNTDFA
                     ATIPEFAGIAANCQIEFRLAQRELSGACTNGITRHASTRTYEGSDAAKLGGWQSGKYL
                     NIWVVKDLTNGAAAYAYYPTSIAGLMYTVDGVICRYDYVGTIGAAGSYAAHVLSHEIG
                     HYLNLQHVWGNSNAPGVACGDDQVPDTPMTEGWTTCNIYGNTCTAPIDNVQNHMEYSY
                     CSTMFSEGQKSRMYAALESDDAMRNNLPTEENLLLTGTNDGYVASTCAPIADFYTSQR
                     YICAGESVTYHDVSYNGEVTSRVWTFEGGSPASSTDIAPVVTYATEGWHQVILNVSNG
                     VGTDTKTWNNYLHVAPAVSIYDENYFEDFNNQTEVENDWILYNKYEDPYEWKWRGNNG
                     YYNTGCIWLNSRFGPDLESDVAISPMYDLSSGLTDNLFFKYSTTSYGDNEDDYTMSLK
                     IYYSINCGDSWIYMNKVTGADLITSYGGSSNFFPQYPDQWAAAAFNLPEACKTDHVQF
                     KIEFLYNPYVNNVFIDDFNFLSGVLNTTEQQASVALKVYPNPVHADGSSTIHYSLTSS
                     NMVTISISDLAGRTIQVIGNAQEQEGSHFITISPKELGLPSGCYFVQIKAGGQLVSDK
                     LIVI"
     gene            complement(17611..>18009)
                     /locus_tag="PLL28_06390"
     CDS             complement(17611..>18009)
                     /locus_tag="PLL28_06390"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF012306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HNF68986.1"
                     /translation="IPPKGIHIFIHGKIQRKTVRFLLDTGASKSVIDQDFANRMFPSA
                     RRIETEHLTTGLGANIPNAVFLKLRGIKAGKYHLKPITFAVIDLSVVNQAYTSAKLEP
                     VVAILGGDILNKYRAVIDYRQRLLQLLVED"
BASE COUNT         5249 a         3706 c         3802 g         5252 t
ORIGIN      
        1 tgagcgtgat ctgctgtata aggtgttgtt tgatatgaaa caagatctca acgatctcaa
       61 gcagtttgtg cttcatatgg cagaggcaaa tcacctgaaa acagatatgt ccatttcagc
      121 agaaatgcac gatcagccta catttaacga gctttcaacc cgccaaccgc agttgcaaac
      181 tgaattcatt accaagcaac ctgtggttat taatccggcc aactcggctg cacatgctca
      241 cgtacatgaa gaagtggaag agagtttgtc gctgatagac aaagaaaaag aactgatcaa
      301 taaggcattg tcaaagcata gaggcaaacg aaaggatgct gctatggatc ttggaatttc
      361 tgaacgcaca ctttatcgca aaatcaaaga atacaatctc gatgaataag cgcagaatca
      421 cgtattcctt agtttgtgtg gcatttctgt tgtcaattgc cgctacgtgt aaagtgtcgt
      481 ttaacggggc tcagattcct gtcgatgtta aaacgcttac catcaattat ttcccaaacc
      541 aggcaaccct cgtttcccca tctctcagcc agacatttac agaatcattg aaagataaat
      601 ttttaagaga atcctcatta actttaaccg aacagggtgg cgattgggaa ttaagcgggg
      661 caatagtgaa atatgaaagc accccgattg cgcctacagg acaggagaca accgcgctta
      721 atagactgag tatcacggtg aaagtagact ttacctcacg taaaagtgaa gatgccgact
      781 ggagccagaa ttttatgcga tacgaagatt atgaaagcgc aaaaacattg agtcaggtag
      841 aaggcgatct gcttcccaag ctttcggagc agcttgccga cgatatctat caaaaattaa
      901 cgcagaactg gtaatgttta ccgaggaatt caatagcatc atcaccaacc cacattcact
      961 tcaggatgtg cccctcgccg atcttaaagt attggcagag cgctatccct ggtgccagcc
     1021 ggtgcaggtg ctgtatgcca gaaagctgtt tttggtagaa tctcctcaat ttgaacatca
     1081 gttacatcga actgcgatat tatcttacga cagggaagta ttattcaagt acatcaacgc
     1141 cgaaatacct gaagtcatca gtgtagcgcc tgagcctgaa gttcaaccgg atcagcctgt
     1201 catagttgag gaacatcagg agattcagcc tgttgttgag atttctgaac cggaacttga
     1261 tcagattagt gtagaagagt ttgatcctgc caccgaagaa ctcattcagg aaatgattga
     1321 aagcattcct gaagtgcatc aggaagacga gatagcacca attgaaatga tgagtgagga
     1381 aacgatggaa ttacaggaag aagaaattga agcagaaagc atgttgcagg aagaaatgga
     1441 tctcgaaaac ctggatcatg ctgtgatcaa tgatgatatg gaagctgtcg cctcaaaccc
     1501 tcttcccgac gagcgtatcg agccggatga ggcctttata tttgagatgc cggcttatga
     1561 tatcgagaca gaattgggca ctctggatga ggccgataag tttacacttc caaacattcc
     1621 accggaagta gccgcagaaa agaatacaga agtgttcacc gattcattca ccggctggct
     1681 taatcgtcta ggtggaagcc ctaccggcaa agtagttgag caaaaggctt ctaatgcgcc
     1741 tgtaaaagta tacctgcgca aacagccaac gccgggatcc gatcctgccg gcagcgccca
     1801 tgaacgcctg atgaatgaac aatatgcagc agaactggct cgtaaaagca ttcaaagcga
     1861 cgatggactt atcagcgaaa cctatgcccg tatccttgta atgcaaggca aatatcagcg
     1921 tgcttcagag atgtatcgca agctaagttt gctaaaaccc caaaaaagcg attactttgc
     1981 agccttaatt gatcagatca acaaaaagat aaaataatat gttgtttacc gtaattataa
     2041 ttattgccat tatcgcagcc gtattgcttg gtttggtagt attgattcaa aacccaaagg
     2101 gtggtggctt atcaggaaca tttggcggtg ccgctaacca aatatttggt tacaaacgca
     2161 caacagatga tatagaaaaa atcacatgga ttctcgtagc tgttgttttt gttctctgcc
     2221 tcagtgcttc tgcattgaaa ccggtagaaa cagttacaca acaaggtgga ggacttggaa
     2281 cagaagctaa aatgccaacc aatgttgctg caccggctcc ggatttaatg gatgaacaat
     2341 ctgcagatca acccggagat actgcgaatt aattgatcaa caatttgaag agataacaaa
     2401 aagcacttcc ttggaggtgc tttttttatg ccattcagtg gaaataatgc cttttatttc
     2461 cctttgtatt tcttttcgta ctgcactatt atctcctcta aagtggggtt gtttgttggt
     2521 ttataactgt cttttaaatc atatccatac attttaccca ggttattcca ccaaaatgca
     2581 gaggtagcgc ccttagtctc ttttaaaata ttatacatcg acgaaccggt attgcgctca
     2641 ataatatcta tcaatacttc tccttgagtt ctggttaatt ttttgagctg tgcctcaaat
     2701 gttttgcgca attgatcctc aagctctttt ttatattgct tttcgtcgtg atgcccgcta
     2761 aatgattgtg attgcaatgc tttgagcgtc attactgctg tatcggcata cggatacacg
     2821 cgttttacaa tagcttcata tttattgtaa ttgtaattct ttagcggtct tgctgttatc
     2881 gtaaatgaat caagttcaac aaaatatatg gtatcagcct cttcatttcc tttaaaagga
     2941 cttccgatct gcgcgtgaac ggcagtttgt ataatcaagg ctatggctaa taaaatggaa
     3001 ttacgcatga taattgagtt acaaattgcg tgctaggttt aatttatttc ggtacaacag
     3061 tatgtaatat cgcatcggta tgttttttgg cctctgaaat acttgattga taggcatcat
     3121 tcaggtaaat ggctttggta tcattgcccg gagctgctga aaccggaact ataatgtgca
     3181 tttttaattg atcggcaact ccacggtata cccaggtagg aacataattg ttatgcgcag
     3241 caagcctgat ttttcctgat ggaagatgtt tttcaaaagt caactgcatg atcacccctt
     3301 catctgtgta ttgatctttt tggttgctga taaaattacc catggaatag gctacaaaga
     3361 cactgtcaac attggacctg cgccctgcaa ctacttccat aggttgtatg acgtgcggat
     3421 gactaccgag cactacatcg gctccgcaat caatcgcatg ctgtacaaat tgtttttgat
     3481 atgcattcgg caaccgctgg tactcttccc caaagtgata aaacacaatc atgagttctg
     3541 cccctttcgc tctgccggcc gccatgtctt ttttgatcaa tgctgaatca cacatattta
     3601 ccagccatgg ttttccatct gcgagcggga gtccgtttgt tgaaaaagta taggaaatga
     3661 taccaatttt aacgccttta atatcgagta tgcgtatgct atctctgtcg ttggctgaag
     3721 caaatgatcc cgtatgtccc aatccgatag aatctaatat ggaaatggtt ctaactattc
     3781 ctttatcgcc ggaatcattg ctatggttat tcgcagtcac caggaaatcg aatcctgctt
     3841 gtttcacagc acgcacatat tggtctgggg tattaaacat tggatatccg ctatagggcc
     3901 gtgcttttcc tgccagagtg gtctccagat ttcctatcgc aagatctgct tgctgcagaa
     3961 tcggtgcaac aagcgaaaaa cttggggtga aatcaaaggt atccacacct gtttttgcat
     4021 agttgaattg ggtgctatga cacatcagat ctccaactgc tgctatggtg aaagtaacca
     4081 atgtatcagg actctcaacc ggttttacag ggatggaaga ttcgccgtta ctacagccca
     4141 tactaagagc taaaccgttg gtcaacagtg ctccgatcaa acttgaaaac atgctttgat
     4201 gaattgccaa acggcgattt tattttttct ttttcttgga gatgaaaatg gagataacgg
     4261 cagccaggat aagcaccgta aatacgatgg tcacaatata aaactgggtc atactaccat
     4321 tgccttttga ctgtgacttc agactaatat tagccacata caaaaggaac aataaggtgg
     4381 tatacctcag tgggcggaag gtttgtttga gcagcataca cgacggtttg ttctttctaa
     4441 ggtaattttt attattcaga aaacgaaatc ggtgaatgtt agtaatataa acgaaaggtc
     4501 atttttttga taaaaatgaa aaaaccctcc gaagagggtt ttctgagata catataacca
     4561 tgacagaata ttacacgatc tcaaatctgt ttatcagacc tgtatcagca tgttcctggt
     4621 tggtagatgc taagtagtat acgcccggag ctgttgtaaa tggaaccaat agggtaacaa
     4681 cctcatcagg aagcagaagg atagtgtcct tccagccttt ttcccattgt tttacagatc
     4741 cgcgtccacc agtgcgatct aacacttgga attgaacacc atagatatgc attacgcgag
     4801 gttcagaacc gcctgtatta tccagcaccc acttttcagt agtgcctgca ttcacagtga
     4861 aattaactgt tccggcatca taagcatcgc cattaatggt gtgcatattg tctgtattat
     4921 ttgttgcttc gtctttcgat atgtttgcat ttgcaatatc aaagttgcgt gtttcacccg
     4981 ttacatcacc ttctgtaagc gttgtaatgt ctgagagatc atcaggcaat gaccatgttt
     5041 catcatctgc agtatcgatt acaaacttca tgatatcgaa acctgtaacg ccctgataat
     5101 cgccggcagc attaaactga ttgcttgaaa gcaaaacctc tgtgccaact gataatgtgc
     5161 tgaaatcgat tattgaatct aacctttctc caggtgccag taatacctgc ttaataccac
     5221 ctccaaggga tttcaaaagg ccaccatcat ttccaatgat attgaagtca agttcagtgc
     5281 catcagtggt agaaatagag agattatata tacgagcagt tgaaccattg ataattcgca
     5341 ggcgatggaa acgtgaactt acatttttga atacgccaat gcgaccattc accaagatag
     5401 tgccgccaaa atacccggtg aggtattcgg ctttatttgg tccgtataac atttggttat
     5461 cttcattgaa acgcttgtct tgcaaagaga tggttgtgct gcgtaagttt ccatttggca
     5521 gattcaatgc atcttcctca gaatcactta caatgaacaa accgccaagt ccaagaccta
     5581 attgatcgcc gccaagcttt tgatattgtg catgatacca atacatacct gcacgattgt
     5641 taaccgtgaa gctgtaagat ttcgaagcac cggaagcaac gccattttca acgccgtctt
     5701 cctgagatgc atccaaggtc aaaccatgaa aatgtaagtt agtgccgaag ctgcttaagt
     5761 tagattctaa tgtcaatgaa atattgtctc cggtgtttgc agtgagtgta ggtccccaaa
     5821 ctgattcaga atatccatag cagaaggtcc ccgatatacc tgcgaaagta tagaagttac
     5881 gatttgcggt aatagttgaa ccattggcaa cttgtgtatc caatgtgttg aacgggctta
     5941 cgaacggcgg ggtaggaact gtagtaccgc caactgtgcc gaacaataca tcatccaaaa
     6001 tgtcctggtt tccatctgaa cagctggcaa gaaatgcagc tggtgttacg gcgatggctg
     6061 tggtgccggc aagtgtgttg cgtaaaaatg atcttctgtt cattttttgt tttttaatgt
     6121 ttgctcaaat gttgtcgtgt gttttgatct tcagagtgcg cgattcggaa actgacccgt
     6181 acgctatcct atcaatcagg gttctcgggg ctaagataat agtttccatg aaagttaaaa
     6241 gttttatatg ttgatttttt taatcacgcc attcccaact ggggatcatt ccccttattc
     6301 ccatgtccac aaccgattca gtagcccccg catcaagcac atcaatatcc catttcccgt
     6361 ctgtatcgga atctatccac tcgaaactat tgttagtgga aaacgaaaaa gtgatgtata
     6421 aatcctgcac ctcatcgccg gttattacca acggttcggc gaacatacct gttactacgc
     6481 aagacccggc aggaataggg gaggttgcat tgataggatt tacaacagtg gtagccccct
     6541 caggagcctg accggtatat atcgcgttgt aggcacttag gtcgccggaa aataaagttt
     6601 caaatcccca gaaaccttgt tctttattcg cgtttactaa agtagtaagg ttttgtatgt
     6661 gcaggtcatt gatgtaagtg ttgtatccca gaaaagacgc taatacaccg tcctgatcaa
     6721 gcacatcacc ataaattggg acattatgca ggtcataaga aattccgaat tgctgatatg
     6781 caacagatgc acgaaggtat gtatacgttc ccggcgctaa tcgctccaat ttgatttgca
     6841 aaaaatcact gccttctgat gctaaaatgg atgaatcaaa aagtattgct tgtgcgcctc
     6901 ccgccagtgt ttccggcgct ttatacaaca ccgctccatc gcctaactgc acgttggaac
     6961 cggttgttaa ttcaatgtaa tgtatactca tcaactgcgt ctccggcgat tgcgagccat
     7021 gtcctgcagg cacttcggat ggttcaccta aattgtcaag acgtgtctgt gtagaatcaa
     7081 atgcaaatga aaaatgaaca tcagcgaata tggtaacatc tttattacaa gctgaaatcc
     7141 atggcaatac tgctataacc ggtatcagaa ttttttttga gggcatttaa atcaattgac
     7201 gtgtattaaa actgaatggc tgcatttgtt gaacaaataa atgaaaaatc ctgcttaacc
     7261 gacttttgct gtcggctaaa agaaatggat cttataattt ggttttcatc atacgtaata
     7321 gaatcggaat gagaaataca tcaggccata ttgccgtaaa tagcgtaaca cccgtcagca
     7381 gaccaatatt aaatgtgaca gatgtagatg aaagcatgag tatcataaaa ccgaaaaaca
     7441 atattgccga catggtgaaa acagcttttc cggtttcctg aaaggtattt tgtaaggcaa
     7501 tttcaagcgg gtagcccttt tgccgttcga ttcgaaactt cgacagaaaa tgtatcgtat
     7561 catccaccgc aatgccgtat gaaattccga aaatgataga agtgggcgca tctaattcta
     7621 tacctaataa acccatgatg cccgcacata cgatcaacgg taaaatattt ggtataatac
     7681 tgatgatcac cattcgccaa tcgcgaaata aatacgccat cacaatacca atacataaaa
     7741 atgcaagcaa gaccccgcta ataatatttt ttctcaaata ttgtgtgttt ttatcgaaaa
     7801 taacaccggt gcctgttatc gtaaatttaa ccgctgaagt atctgttttt tcttctatga
     7861 atttctttaa atcggtttga aacatgcgaa tgctatcggt gccgacatca ctcaaaaaac
     7921 cactaattct tcctgctttt ttatcgctcg agataagtgc atgcatttcg cgtgttgcgt
     7981 atttgtctat caacttttga taaccataca atatgctatc attttcgggt aaacgataat
     8041 aagaagcaat gtcgccatta tatgcacgat gcatgctctt gtatggcatt gtcatcccct
     8101 gcaatccatt tacaagatca aatgatgatg catatgtttc aactttttct acgtccctta
     8161 ttattgccgg ttcagtaatc aaatgcttgc ctttcgcttc tgctgcaatt tcaagcggac
     8221 ggaagccgtt aaagtgctct tcaaaaaaat gaaaatcggt tgtaacctgc gccccttttg
     8281 gcattccggc ctccaggtga atatttgtgc taatctgagt aacgccataa ataaatatca
     8341 acattgcggc actcataatg attaaaatgc gacgctttct tcgaattcct aaggtataaa
     8401 tagcattaat aaaagcagtc cattttttgt cctcaccatc agttttcgca tcgagttgat
     8461 caaggcgaaa caaggtaagc accggactga taaaaaataa aacaactgca tatacaacca
     8521 aaacacccaa cgcagaatat aatccaaaat cttttattgg tggaacattg gaagtgagca
     8581 tggttaaaaa tccaattgcc gaagtaacgg cagttaacca tgtggcaaaa cctatgtctg
     8641 acaaagtgcg aaataatgca acttttctat tgttgttttt tcgtaattca gtgatgtatt
     8701 ttcctataaa gtgaactgcg tcggaaatac caatgatgat gatgacaata gggtagagtg
     8761 tactcatcac attttgttcg attctcaatc cacctaaaat tcctgtaaat aaaataagtg
     8821 aaattgcaac agtgagaaca gataagacta cacaccatat ttttctgaac atgaggaagg
     8881 taactattgt aaccaacaca atgctaagca tggcataaat taaaaattcc tttttctgga
     8941 tttcaaccag ctcgacctcg aaattggctt tccctaaaag atgataatcc ttaattccgg
     9001 catcatgcat cgcagctctg acatctgcta caaatgactt tgccttttgt tgactaagtg
     9061 agtcatttgt tttgatgaga ataacaaccg tagaaaaatc ttcagaaact agtttgccaa
     9121 ctacacgaac atcggcagcc agtcgcaagc tgtctgattc aaatcgactg ctatcgtccg
     9181 gatgaactgc cggatagtcg atgaacccga aaggtaattt aacaaaatat ctgaagtttg
     9241 tcacagaata ggccctttcg gcaccctcaa ttctgccaac cgcatttgta gccgaatcca
     9301 gtgaggtcag gaacttggca tcaaagagcc cattctcggg ctgcattccc accagcaaaa
     9361 agttgtcgtc cggttcaaat tgctgtcgga aagcatcata aaaggccaga tccgggtcat
     9421 tttccggaaa aaaatcttcg taatcgtagg aaaaccgtaa ttgcagcgta aaaaacactg
     9481 ccagaagcac caacaggctg aaaaccgcaa tcacatatac cggtttcaaa ccggcaaccc
     9541 atttccataa tttactgttc gataccacct cagcgcaaat ttacgatgga gaatccggat
     9601 tggtttgtaa gaccttgtgt ctcagaaaaa tgtcaggaaa aacctatttg ttcataacta
     9661 ggtcacaaaa gtttgttttt acatgctaaa actgttacct ttagtcgtga ataggtctga
     9721 tttctaacca ttaaataaac cgaaacatgc ttatgaagca attgttacaa aagctaacat
     9781 tctctctcct ccttgtttca gctgtcactt ttatggcttc tgcccaaaca ccggacagag
     9841 gtaatgcaac cattgatgcg aacatggtaa tccatttgga tgaaacccaa ccgctggttg
     9901 ccgattattc attttccttt agtgcgatag ctttcaagaa tgaggccgct gctactcgat
     9961 ttttcgggct ttgccgcgac aatattgtgt cctacaacct tgactgggct gcaaaaacag
    10021 caacagtgca cattgcattg gaatacatgg aaccaagggg ctgggacatt gtgaaataca
    10081 atgactattt cagtaagctc gccgaacgct atcgtacaac ccttagcgtg gtgaatgaat
    10141 aatatttttt acaactgatc aaatttttta ttacatgcaa agcagaataa ttgctgttgt
    10201 cgcaatggtg gtattttcac tcttcaccat caatagcgca accgctcaaa tcgttggtgc
    10261 taacgtattt ttgaaaggga attatgtgga agtaggtatc aatacttgcg gagcttacgg
    10321 aacaccttct gccccgcctg caggctatca tccaacagaa ggcggacttg gattcgttgc
    10381 cgattgggaa tctgacggat ggggttcagg aactccggta tattgtggcg attacttcgt
    10441 accgggttct ccggttgaag gatggcagct tcaaattgga cctacctggt ggacgaatac
    10501 cgaccaaagt tgtggcggtt ctgatattcc cggtgatgtt tcagattata catatgcagg
    10561 cggtgtttat acaggtgttt ggacaggtaa cctgactacc ggaactaccg atctggatat
    10621 tactcagata actacactcc ctgaggatgc attgtatttt gttacccgaa tcctattgtg
    10681 taataacggc gcagaccctt tgaataatgt ttattacaaa agaaatgttg acccggataa
    10741 tgatcaacct tggagtggtg attttactac cgataacgtt atcgtttatc aaccaccaac
    10801 cgacgaagag gctttggtga cttcagaagg ccttacttat ggttgtttcc tcggaattgg
    10861 tgcacgcgat gaaaatgccc gtgtatctta cggaaacttt ggaaccgacg acggctctcc
    10921 aaaagcagtt tgggatgcaa ccggcggata tgttggaaca ggatcagaag ttggcgatat
    10981 tgctaactca atttcgtttt acattcctgt tatcaatccc ggcgaatgta aatgcgtggc
    11041 ctttgcttat atcctcaatg ttgatgacct cgaggcagca ttggaagcaa cagtaactta
    11101 caaccttacg gcagatgata tcaccgttgc atcaggcgac acagttcgca cttgtaatcc
    11161 gggagattca gttgaactga gtattttggg tgctgatgat tatttctgga catggacacc
    11221 gacaacaggt cttagtaccg atacaggtat ctctgtaata gctactcctg atgttacaac
    11281 tacctatacc attaccggtg ttggcggatt ctgcggtgat gcaacattgc aggtaacagt
    11341 agaaattgat acgcatgaat ttgcagatgc cggtgatgat gtagaaatat gcctgggcga
    11401 tgtcaccacc cttacaggcg acggcggtcc atacgagaat atttatgctt ggacgcctgc
    11461 cgctgatctt ggctgtgctg attgtgctac aactacagca acacctacaa ccgacggtgt
    11521 atttacttac actttaacaa cctacgacat ttataactgc cctgctactg acgaagtaga
    11581 agtaacagtt caccctctcc ctgatgtaaa cgcaggtcct gataaggatc tttgccctga
    11641 aggtgcagtt gacctggaag ctacaggtgc aattgaatat acctggagtc cgtctgacgg
    11701 attaagctgc gatgattgcc cggatccgac ttgtactgtt gatgaaaata ctacttatac
    11761 cgttacagga acagatgcaa atggttgcgt ggctacggat gtaatcaacg taaacgtatt
    11821 ccctaatttg gaaggtatca tgactgtaga tcctacagaa ataaccattg atgtatatca
    11881 aaatgaaacg gctcaattga atgcttatgg tgccgaaatc tatcaatggt ctcctccaat
    11941 gggattgaat aatgtcaata tctctaaccc gattgcgcaa cctgccgata ctacagttta
    12001 tattgtaaca ggagttgatg ccaatggatg taccgatgta gataccgtaa ctgtaaacgt
    12061 aatcggttct ctggaaatag gcgttcctac agcattctca ccaaacggag atggcaccaa
    12121 cgacttctgg ttcccaatga tatccggttc cggttgtgtt gaaagctgga cagtgtttaa
    12181 tcgttatggt gaagttgttt tcgagggcaa tggattctgt ccaggcgagg ttacgcaagg
    12241 ttgggatggc acttataatg gagaattgca aggaattggt acctatgtcg tgatcattaa
    12301 agctgctacg acattgcttg aagatcgatt tgtgcagggc aatttcacat tagtacgtta
    12361 atattgaatg gaacccgata ttcatgaaca caaacaaata ttgctaaatt tataaacgga
    12421 atgatgttaa gggcatcatt ccgttacaaa ccaataaacc ttactaatga aacacttaca
    12481 cagattactt cccctcctga tcttgcttac atgcacacaa atgcaagctc agatagtagg
    12541 tgcaaacgcc tttctgaaag gtaactttgt tgaggtgggt atcaatactt gtggggcata
    12601 cggctctaat ggagctcctc ctgcaggtta tcacccaaac gtttttagcg gcctcggatt
    12661 tgttgccgat tcagatatgg atggctggac aaccggtaca ccggattatt gcggcgatta
    12721 ctttgtaccg ggttcaccgg ttgagggatg gcaagtacaa ataggtggta ctgtatatac
    12781 gaacaccgac caatactgtt ggacttcaga aattcccggt gatataacat cgtatgatta
    12841 taccgcagga atttataccg gtatttggga aggcaccatt gctgcagaag atgtgacaat
    12901 tacacaaaca actacattgc ctgagaatgc ccgatacttt gttacccgta ttctcatttg
    12961 taacgatgga ccaactgacc ttacagacgt atactatatg cgaaatgttg accccgatca
    13021 ggatcagcca ttcggattta gctttatgac taccaatagc gttattttcc aacctcctgt
    13081 tgaccctgat gcattggttg aatctgttgg tgatgtatat ggttgctacc taggtatggg
    13141 tgcacgtgat gaaaatgccc gtgtaactta tggttgcttc agcactacag acggaacccc
    13201 tgctcaagcc tggtcaggcg cttcagtttg tgcatggagt tcaggatatt cactttcggg
    13261 aacctattat tgtgattgtg ctactcaggt aacttttaaa attccattga tcgctgctgg
    13321 tgaatgtcaa tgtgtcgctt ttgcttacat tctcgatgcc gacgaccttg atgaagcgct
    13381 agaagcaact tcctcaattg gtgttgccgc agatggtgta gacatctcaa cttctggtat
    13441 tactttgatt tgccccggtg attctgttga actgaatatt atcggcggtg aagactatga
    13501 ctggacctgg actccaacaa ccggtttgag ctcaggtaca ggcgatacag tggttgcttc
    13561 accagatatc accactactt atctcgctac cggtgtaggt atttgtggaa caatagaacg
    13621 cgaagttaca gttgaagtgc caccaccacc aattgcaaat gccggacctg atatgtcttt
    13681 atgtccttcc gatacaattc acctggcagg aagtggtggc gttcattacc aatggactcc
    13741 tcctgtttat cttgatgacg acacgctttc aaatcctgcc gttatggatc cgctgacgga
    13801 tatgtattat cagttagtta tttgggatgc aaatggttgt accgatacgg atgatgtaat
    13861 ggtaacttta tatgatttac ctatcgtaga tgccggtgat gatcaatata tggctgaagg
    13921 cggatttgct caacttaccg catcaggtgc cgctacttat gtatggacac ctgaagattt
    13981 ccttactaac gcactgatct acaatccaat cgctcaaccc gaagatacta ccatgtatta
    14041 tgttgttggt actgatgcta atggttgtga aaatattgat agtatgatcg tttatgtgtt
    14101 agaccaaaca catatcgtag taccaaacgc attcacacca aatggtgacg gattaaatga
    14161 tacttggaaa cctgttttga taggaattgg tgaaatcacc tatttctctg tgtataacag
    14221 atggggagat ttgctgtttg aatcatctga tccaacaatc ggatgggatg gaacctatca
    14281 agccgatgaa caagaaattg gtaactatat cgtaatggtg aaaggtcttg acggatttgg
    14341 tcagccactt accaagtcag caatggtgtt gttgatgcga taaaaattgc atgcaaaata
    14401 tgtaatcccg gtattgcaaa atgccgggat ttttttttgg aaaacgtttt gatatttgtt
    14461 gactgccatt aaaaggcaaa taagaactat tcacagatat tgttacttat acattgcaat
    14521 atccgcacgt ttcatcagtg cagttgcttt ttagctctcc taagtaagta attatcagga
    14581 tagaattgca atccggtatt aaatgcattc ttagctttat gtttttctcc aatatgttca
    14641 tacaataatc ctaattccag aaaccagtcg gcacgtgagc tcagcgacat cgcttgcttg
    14701 taatcttcga tggcattttc ccaatcctta ctcttcgcga ataatgtagc tctggcaata
    14761 taaaatacgg tgtttgcagg atttaactga atggccttgt cgtagtatga taaagctttt
    14821 agtaacattt cagaatttcc gttgaacgca taattccaag aaatcacatt tgcaaggtga
    14881 taccaggtat cggaagaatc cggatacatt gttaccattt gaaacattgc tcttgttgcc
    14941 tgttggtaaa aagcagctat aagtgcttca tctttagata aaacataatt attgcaagct
    15001 tcataatgat tgtaattcaa actatcgagt tgcaccaggc tcaatctatc attcatggca
    15061 atgatccgca tgttatccga gtgaaattgc cctgtataaa cagcctgctg agataaaacc
    15121 gtaatgtcgt ccggtgcccg ctcaattgct cgctcgtaat cgattaatat actgtcttgc
    15181 gggatgttac ctaatgcaat atgaatagag gctctggcaa tataataata tgggaaatca
    15241 ggatcttcat ttattaagcc atttagtgca tgaagggcct ttaacgtatc cgcccgctct
    15301 accgcatcat aataatttgt gagcaagggt ttggtggtgc tattaataat ctgtagcggt
    15361 tgcgcatagc tatgtaccac gcacagcgaa acaaaaatta tcatcaatac cggacggatg
    15421 cgcattattg cttacttgat aggtcaaatc tactgcaatt gttggttgtg aaagtaaggc
    15481 ttaacataga ttcgttattt ttatggtcta gttactcaaa gtgatgaccc agatacgcct
    15541 atccttgtta tgcatgttat tatcatgcct ttcaacatcc atgcttcatg cccagtcact
    15601 gttaacatgc ggcactgaca tcgaacgcag taagttaatt gccgaccatc cggaaattct
    15661 ggatagagaa gcaatatttg atgctttagc agcgaagaaa tttgctgaag tggcacgtat
    15721 cgagggtgat gttttggtga taccggttgt gtttcacgtt gtacatgatt acggtcctga
    15781 gaatatcagt gatgcacaaa tatttgatgc cattgagcat atcaacgctg attttagcgc
    15841 aacgaatacc gattttgccg ctacgatacc tgaatttgca ggaatagcag caaactgtca
    15901 gatagaattt cgcttggctc agcgtgaatt aagtggtgct tgtacaaatg gtattacgcg
    15961 acatgcttct acgcgaacat atgaaggttc agacgctgcc aaacttggcg ggtggcaatc
    16021 gggaaaatat ttaaacatat gggtagtgaa agaccttaca aatggcgctg cagcttatgc
    16081 atattatcct accagcatcg caggacttat gtatactgtt gatggcgtta tatgccgata
    16141 tgattatgta ggtacaatcg gtgctgccgg atcctatgcg gctcacgttt tatcgcatga
    16201 gatcggccat tatttgaatt tgcaacacgt ttggggaaat agtaatgcgc ccggagtggc
    16261 ttgcggtgat gatcaagtgc ctgatacacc tatgacagag ggatggacca cctgcaacat
    16321 ctatggcaat acctgcacgg cacccataga caatgtgcaa aatcatatgg agtattctta
    16381 ttgtagtacc atgttttcag aagggcaaaa aagtcggatg tatgccgcat tggaaagtga
    16441 tgatgcgatg cgtaataatt tacctactga ggagaattta ttgttgacgg gaaccaatga
    16501 tggatatgta gcatcaacat gcgctccgat agcagatttt tatacttcac agcgatacat
    16561 ttgtgccggc gaaagtgtca cctatcatga tgtaagttat aatggtgaag taacatcacg
    16621 cgtctggact tttgaaggag gatcgccggc atcatcaaca gatattgcgc ctgttgttac
    16681 ttatgcaaca gagggttggc atcaggtaat actgaatgtg tcaaatggag tgggaaccga
    16741 tacaaaaacc tggaataact atttacatgt tgctcctgca gtttcaattt acgatgagaa
    16801 ttattttgag gattttaata atcaaaccga agttgaaaat gattggattt tatacaataa
    16861 gtatgaagac ccctatgaat ggaaatggcg cggtaataat ggctattata acacaggttg
    16921 tatttggctg aacagcagat ttggtccgga tttagaatca gatgtggcaa tttcaccaat
    16981 gtatgatctt tccagtggat tgacggataa tttattcttt aaatattcta caacttcata
    17041 cggcgataat gaagatgact acacaatgtc gttgaaaatt tactattcta taaattgtgg
    17101 tgacagttgg atatatatga ataaagtaac cggcgccgat ttgattacaa gctatggcgg
    17161 aagcagtaat ttctttccgc aatatcctga tcaatgggct gctgctgcgt ttaatttgcc
    17221 tgaagcttgt aaaactgacc acgtgcaatt caaaattgaa tttttataca acccttatgt
    17281 aaacaacgta tttattgatg attttaattt cctttcaggc gtattgaata caaccgaaca
    17341 acaagcttca gtggcattaa aagtatatcc aaatcctgtg catgctgatg gtagttctac
    17401 cattcattac agtttgactt catcaaatat ggtaactatt tccatcagtg atcttgccgg
    17461 taggactatt caggtgattg ggaatgcaca agagcaggag ggaagtcatt ttataactat
    17521 ttcaccaaaa gaacttggcc ttccttcggg atgttatttt gtgcagatca aagcaggagg
    17581 tcagttggta agtgataagc ttattgtaat ttaatcttcg acaagcaatt gcaataatcg
    17641 ctgccggtag tcgatcacag ctcgatattt gtttaaaata tcgcctccta atatggctac
    17701 aacgggttcg agctttgcag aggtgtaagc ttgattcact acgctaagat ctattacagc
    17761 aaatgtgatt ggttttaaat gatatttccc ggcttttatg ccgcgtaatt ttagaaatac
    17821 tgcatttgga atattagctc ccagacctgt tgtaagatgt tctgtttcga tacgtcgtgc
    17881 agaaggaaac atgcgatttg caaaatcctg gtctatcaca cttttactcg ctccggtgtc
    17941 cagcaaaaag cgaacagttt ttcgttgaat ttttccgtga ataaatatgt gaatcccttt
    18001 gggaggtat
//
LOCUS       DAOQHY010000057        18003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1447_length_18003_cov_5.161076, whole genome shotgun sequence.
ACCESSION   DAOQHY010000057 DAOQHY010000000
VERSION     DAOQHY010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 18003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18003
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1447_length_18003_cov_5.161076"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..1486
                     /locus_tag="PLL28_06395"
     CDS             260..1486
                     /locus_tag="PLL28_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNF68987.1"
                     /translation="MNKRKIINDPVHGFITIPHEIIFDLIEHPWFQRLRRIQQLGLSH
                     LVYPGAIHTRFNHALGAMHLMGKAIHVLRQKGQQITEEESEAAAIAILLHDIGHGPFS
                     HALEKTILSNVHHEEVSIRFMERLNEQFQARLSMALEIFRDTYPKRFLHQLVSSQLDV
                     DRLDYLTRDSYFTGVHEGVIGYDRLIEMMDVENDELVIEHKGIYSVEKFIIARRVMYW
                     QVYLHKTVICAEQMLIKTLNRAKYLAKSGTQLFATPSLHFFLYRDVATRDFDDDADIL
                     EQYALLDDVDVWASIKEWQHCDDRVLSFLSKSLINRDLFRIELGNEEISASRLADIRA
                     KVQSAYKITDPFDLQYFVFNDVTTNSAYNLEANNINIKFRSGRILDVASASDHLNLAA
                     LADTVVKHYICYPKET"
     gene            1491..2543
                     /gene="lpxD"
                     /locus_tag="PLL28_06400"
     CDS             1491..2543
                     /gene="lpxD"
                     /locus_tag="PLL28_06400"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNF68988.1"
                     /translation="MEFTARELCDFLQGTLEGDPSIKVRKPARIEEGQPGDVCFLANP
                     KYLSFAYTTAASVLIVNADQVFEQPVKSTLIRVKDAYTAIAKVLDQYQNMQRESAAAN
                     HKVESNAIVSPSAKIGAKVHVGSFSTISEDVVVGDNSIIHAQVYLGKRVKIGANTCIF
                     PGVIVLDECVIGDNCIIGAGTVIGSDGFGFAPQADGTYKKVAQTGNVVIEDMVEIGAN
                     TTIDRATMGSTIIRKGAKLDNLIQIAHNVEIGENTVIAAQSGISGSTKIGKNCVIGGQ
                     VGIVGHITIADGTKINAQSGVSKSVKEPDQALTGSPAFEYSAGMRSQVLFRKLPELMD
                     RIKLLEEKISQLENKQ"
     gene            2563..3969
                     /locus_tag="PLL28_06405"
     CDS             2563..3969
                     /locus_tag="PLL28_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HNF68989.1"
                     /translation="MNKEYQHTIKQSVTVSGVGLHTGKEVDMTFHPAAVNHGYKFRRI
                     DLPGSPVIEADADLVTDTARGTTIAKNGASVSTIEHTLAALVGMGIDNVMIDLNGPET
                     PIMDGSSKEFIEAIHSVGAVEQEAEREYINIAENITFKDANRKTEITVMPADDYQVTV
                     MIDFDSKVLGKQHATLDHISEFSGNIGNSRTFCFLHELEMLYENNLIKGGDLNNAIVI
                     VDKEVTNDEMEKLKKMFNKESVSVKKEGILNNLELHWSNEPARHKLLDVVGDLALAGK
                     PIKGKVIASRPGHPSNVAFAKKLKAYYKLRRTQMVGPHYDPNAEPILDINSISKMLPH
                     RYPFLLVDKIIEMGENHIVGLKNITFNEAFFQGHFPNNPVLPGVLQIEALAQTGGIFV
                     LSKVPDPENYDTYFLKIDKVKFKRKVLPGDSLLLKMELMAPIRRGICEMKATAYVGNN
                     IVTEGELTAQVVKRNPTA"
     gene            3966..4754
                     /gene="lpxA"
                     /locus_tag="PLL28_06410"
     CDS             3966..4754
                     /gene="lpxA"
                     /locus_tag="PLL28_06410"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNF68990.1"
                     /translation="MNQPLAYVHPQAQIADNVVIEPFASISKNVVIGEGTWIGPNVNI
                     MPGARIGKNCRIFPGAVISAVPQDLKYRGEETEVVIGDNTTIRECVTINRGTKYAFRT
                     VVGSNCLLMAYSHVAHDCVIGNNVILANSVNLAGHIEIGDWVILEGLVAVQQFIKIGA
                     HSFIAGGSLVRKDVPPFVKAGREPLSFVGVNSVGLRRRGFAAETIEHIQNIYRLLFVR
                     GYNVTKALQYIEAELPASAERDMIVTFIRNSKEGVMKGFRHINE"
     gene            4751..5368
                     /locus_tag="PLL28_06415"
     CDS             4751..5368
                     /locus_tag="PLL28_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF68991.1"
                     /translation="MIISLENISKRFNYEWIFRDVNFAFDENDRCAIIGPNGSGKSTL
                     LKVIAGQLTPSSGNIIYSDTQEIPQENVYQMVSYAAPYLELIEEFTLSEMVQFHFSFK
                     KWKSGLNLQAIISLSGLEKHKHKQLRTFSSGMRQRVKLVLALCAETPLVILDEPTTNL
                     DESGVEWYLHLIRDFGVGRIILVGSNQEREYTFCSKSLNILDFKG"
     gene            5794..10596
                     /locus_tag="PLL28_06420"
     CDS             5794..10596
                     /locus_tag="PLL28_06420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HNF68992.1"
                     /translation="MYTTFGIQANGFISMGAVPSSSYTAISSGSSNNIISAFNADLEG
                     DAVAAPGAKLMYQLSGAAGSQVMTVEWKNWGFWSAGLNECSFQIKMYETSNNVEIVYG
                     SAPGTTSKTLQVGLRGASSADYNNRTTTTNWSATTAGASNSASCTFSSTVKPSSGLTF
                     QWTPPTPCTGTPTPGTIPATAAVCSGAATGVTLTATGYTTGVSGIAFQWEESDDNGVT
                     DTWANAVGGTGATTASYTTPALASTRYYRLKVTCTPSTLFDYTGSCMVSVTNCTFDVT
                     YTTGTLFTSIMPAAGGTGSAMPGWLGTSGDDNTTTTVDLTGTSFTYQGAPVSGFRACT
                     NGWMSFNTATTTTTFSNSLSSTSQSKVLAPFWDDLVLTGNNYANRDNCMRYKIDGTLG
                     SGTAVITMEWAGMERFSNPGPNWNFQVKLYEAGNYIEYIYGTFEGFDGTNYTSHTYSI
                     GYNGSNPAGTGAADRFALQTVLTNHFSSASDPASHAVNPYCFSKYTLTPGTYTGPVTA
                     PAIPVPTNNESAGAYTLAVGTAEVTSPNFCSTYYTSRGGTNSGMGAAPCATAGNEDDD
                     VWFKFVTTGVTDYNIRVRPSPGYDAVVQLYDASFTSIGCANAVGAATSTTAGTETIAA
                     TGLTAGGTTYYVRVYANSIGSGTSGEFSINVYQSTPIPANDDCAGAFTLAVGTSCSYT
                     SGTSLTATSSAGEVAACTGTADDDVWYKFVATATNPVITVQSGAAFNAVLSVYSGTCG
                     ALANIYCINATSTAGIETVNTTGLVIGQTYYIRIYHSAAGAGSGSFQVCVYNPVPSCT
                     TYISPADAAVGIVGAGTTLQWNAATYATSYDVYFGTVTPPPFATNTASTSWSTGALAP
                     LTTYYWSIVPKNDLGDPGSCSIYSFTSVPECTGTPVGGAAASTAASVCGTGTSFTLSL
                     TGASTDAGIAYQWQADAGAGYADIIGATSSTYSTTEAGTTSYQCVVTCTPSGFSATST
                     PVSVTFNPAPTGDVLADPIIIASLPYTATGNNLSSNCWTNQIGQSSPDVYYRVIPECS
                     GTLNLNLCSGGTISDTYIHIADATGTILASNDDNGPSCAGLLSSINYAVSGGVTYYII
                     VEGFSANEGTYTLNVSNSATYSVWYADADSDLFGDAFSTTTTCDGFAPSGYVGNNLDC
                     NDADAAVNPSAIEVCNGYDDECDGLTDDADPDVIGFSVWFADADGDTYGNAAIIAEAC
                     AAPVGYTADPSDCDDTNSAINPGATEICNGGIDDNCDGLADDADPGVVGAPSWYVDAD
                     GDTWGDAATAVSACIAPGGYIADGSDCDDTQILYTDADGDGHGTATPAPCGATNTDDC
                     DDSQSTVYPGAPELCDGLDNDCDATIDEGTVTATVTPAVGAVTCKTYPYTFSANTGVG
                     YTYQWFKNGNLVVGATGPTLATDKPGYYQVQVNVPGGCFAMSDASLLTVNPLPNATIS
                     APNGTSLCAVVKLKVSENATWTYQWYLEGTPIAGATNFLYYPSVAGNYYAIVTSATGC
                     FRQSATLTVTSCRDAEVSGNETMNVYPNPTSSMFNIDLMMADGYNGTADVYVTNVLGD
                     IVLNKQLTVNNGAVSAELNLGVDAAAGMYLVKVVAGGKQFNQQLTVVK"
     gene            10669..11814
                     /gene="nhaA"
                     /locus_tag="PLL28_06425"
     CDS             10669..11814
                     /gene="nhaA"
                     /locus_tag="PLL28_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HNF68993.1"
                     /translation="MKLTKLFTEFFESERTGGIILICCTFVSLLIANSALGTGYNAFW
                     HHEIAGHSIVHWINDLLMTFFFLLVGLEIEREIYIGELKEFRKASLPIFGAIGGMAIP
                     ALVYIGINISSDNMNGFGIPMATDIAFAIGILSLLGNRVPASLKIFLTALAIIDDLGA
                     IIVIAIFYSQSVSLLYLGAALTLFMIMIIMNRMKVMRLYLYLIPGILLWYCMLQSGIH
                     ATIAGVLIAFAIPFREGDNHSPSYKLQHFLHKPVAFFVLPIFALANTAIVLNTQLFSG
                     LQSTHALGIAFGLIFGKPIGILLMCYLAVKLKIAGLPEGVNWKQLAGVGLLAGIGFTM
                     SIFISLLAFEDDQMINISKLAILISSIIAAVTGLWYLSRQMDKQISA"
     gene            complement(11967..12395)
                     /locus_tag="PLL28_06430"
     CDS             complement(11967..12395)
                     /locus_tag="PLL28_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNF68994.1"
                     /translation="MIYKRNASDFMTGNVIVAGVNNTFDQVMEFFTRHKIQHLPVAEG
                     TRLIGIISIKDMLTFMQDYLAGSGDVSKAALNAAFSIEKVMTANPTTVSPDTPQKDIL
                     EILAAGKFQAVPVVKSGDLVGIITNKDITRLYHYDATHIL"
     gene            complement(12535..14598)
                     /locus_tag="PLL28_06435"
     CDS             complement(12535..14598)
                     /locus_tag="PLL28_06435"
                     /inference="COORDINATES: protein motif:HMM:NF015530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68995.1"
                     /translation="MNKSIMTLLFGGVICFTAQAQGLQQVTGTSQQYSVNSADEPRMG
                     DSFVWNTEQFADLGILRYTIPDFDKLTLNQKLLVYYLTEAGYAGRDIIWDQNYKYNLT
                     IRKALEHIVENYTGDKQSKEWQDFMIYTKRVWFSNGIHHHYSTDKIMPGCSKAYFISL
                     VKTTGAQLDTKIIDIMFDPVVDNKKVYLQPDKDLLLNSASNFYADDVSEKEVEAYYAA
                     QPGMNDEKAISHGLNSRMIKTDKGLVEVVYKADGLYGDAIKQIIANLKLAMPHCENDM
                     QANALRLLIKYYETGDLKVWDEYNIAWLQDTHGDIDYINSFIEVYNDPMGYRGSFESI
                     VEIKDFEASERMQKLAEHAQWFEDYSPIMKSHKKDNVVGVSYRVVNVAGEAGDASPST
                     PIGVNLPNSNWIRADYGSKSVSLGNIVYAYEQAGNSSLLSEFCYTEEERQRAVNYGSL
                     AAKMHTAMHEVIGHASGKINPGVGTPKETLKNYANTLEEARADLVALYYTMDPQLVEW
                     GLIPSLEVGKAEYDSYIRNGLMLQLRRIEPGKTIEESHMRNRQMIAAWVFEKGKSKNV
                     IERKYENDKTYFVINDYEALRQLFGELLREVQRIKSEGDYEAGMKLVETFGVQVNEEI
                     HAEVLARTANIKTPPYWGFINPQLSIVKNDQGEVTDVTVSYPEDFTQQMLYYGKHYSY
                     LKGGQ"
     gene            14712..15011
                     /locus_tag="PLL28_06440"
     CDS             14712..15011
                     /locus_tag="PLL28_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68996.1"
                     /translation="MKKKLLLSILVVMAGTIAAVYAVNHRSEAPTQIAAPTEQVSAEN
                     PAHCDPSNCSPEEAAKCPYANKAAQATTESADNVCPATKECPPSKCNSASKTATL"
     gene            complement(15342..15938)
                     /locus_tag="PLL28_06445"
     CDS             complement(15342..15938)
                     /locus_tag="PLL28_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HNF68997.1"
                     /translation="MEYVEHLSKDPVMAAMIKKHGAFTLKKQKDICTYLCYSIMSQQL
                     STKVADVFHARFDKLYDGKPTAEKIMATSIETFRGMGLSQAKANYVINVATFAQTQGM
                     EVKQLQAMDDMEIIKYLTQIKGVGRWTVEMLLMFALGRKDVFATDDLGIQQAMIGAYG
                     LRPRDKKKFRLRMEKIAEQWRPYRTYACMHLWRWKDNG"
     gene            15951..16355
                     /locus_tag="PLL28_06450"
     CDS             15951..16355
                     /locus_tag="PLL28_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF68998.1"
                     /translation="MKIELFSSPDYFHTMSATESNTISSKMWFWSHFALVVIAWIGPF
                     IFDWYLMVTGYALVVIQFAVFNRCLMNDKHDLAEEGDMTFYAQLLESIGFNFSRAKVK
                     RIVRPWIYVFLAVFVIFLQVIFSYVPVLHIPG"
     gene            16408..>18003
                     /locus_tag="PLL28_06455"
     CDS             16408..>18003
                     /locus_tag="PLL28_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNF68999.1"
                     /translation="MKKIFILGACGLFLSIAGAQGVMTPELLWSLGRVGAETVTPDGQ
                     HVIFGVTYYDIATGVGERNLFSIGLTDHKPIQLTTTKGGENSVMTLPGGKMGYIYQGQ
                     IWQSEWDGSNAKQLTNYPDGLSNVRFSPDGKHILFSKDVKLIDVLASEKYKDLPNSSV
                     RIYESLNYRHWDTWEDGAYSHIMLATWDNGIIGPEKDIMDGLPFDSPQMPFGGVEDFM
                     FHNDNDHIIYVTKALHGTDYTQSTNTDIYLYTMSTGDTKNITAGMEGYDTNPNFSPDG
                     NRLAWLSMARNGYEADKNRLYINDIKNKVVIDASESFDESIEALRWSNNGNFIYFLSP
                     INGTLQLFELDVTNVFTKVDGRKRNPIRQVTSGDYDVASIVGETNGMLVVTRTDMNHA
                     AELFLVNPSTGVMTAITEINKPNYDKIGMCKTERKMVKTTDGLDMLVWFIYPPNFDPN
                     KKYPTLLYCQGGPQSALTQFYSFRWNFQLMASQGYIVVAPNRRGMPGHGTKWNEQISK
                     DWGGQAINDYYSAIDYAKTLPYVD"
BASE COUNT         5088 a         3798 c         3825 g         5292 t
ORIGIN      
        1 aatatcagat ccaattccgg actgacacat ttgtctacag catccagtcc attgctcacg
       61 gctacaacat catatccctt ctcttcgagg aacatgatct gtggttttag catatctatc
      121 tcatcatcag cccataaaat ggtgattccg ttcttcataa atccctaatt ttgcgttgtt
      181 gttattggta gcgtaaagat acttcgataa atacttaggc gttggcgcag cctctcacat
      241 cacgataaaa actgcagtat tgaataaaag aaagatcatt aacgatccgg ttcatggatt
      301 tattaccatc cctcatgaga tcattttcga cctcatagaa catccatggt tccagcgttt
      361 gcgcagaatt cagcagctgg gactcagtca tttggtatac cccggtgcta tccacacacg
      421 cttcaaccac gcattgggcg ccatgcacct gatgggtaag gcaattcatg tgttgcgtca
      481 gaaaggtcag caaattacag aagaagagtc ggaagccgcc gcaattgcca tcttgttgca
      541 cgatatcggt catggtccct tttcgcatgc tcttgagaaa actatattaa gtaatgtgca
      601 tcacgaagag gtgtccattc gcttcatgga acgcctgaat gagcaatttc aggcgcgatt
      661 gtcaatggca ctcgagatat tcagagatac ttaccctaag cgctttttac accagctggt
      721 gagcagtcag ctcgatgtcg acaggctcga ttatctcacc cgcgacagct attttaccgg
      781 tgtgcatgaa ggcgtaatcg gttacgacag actcatcgaa atgatggatg ttgaaaatga
      841 tgaactggtg atcgagcata agggcattta ctctgtagaa aagttcatta ttgccagaag
      901 agtgatgtat tggcaggtat atctgcataa aacggtgatc tgtgccgaac aaatgctcat
      961 caaaaccctg aacagggcga aatatcttgc aaagagcgga acgcaactgt ttgcaacgcc
     1021 atccctgcat tttttcctgt atcgcgatgt ggcaaccaga gattttgacg atgatgccga
     1081 tatcctcgaa caatatgcct tactggatga tgtggatgta tgggcctcaa taaaagaatg
     1141 gcagcactgc gatgaccggg ttttatcctt cttgagcaaa agtctcatca accgcgattt
     1201 attcaggata gaattgggaa atgaggaaat ttccgcttca agattggcag atatcagggc
     1261 gaaggtgcaa tcggcataca agatcaccga tcccttcgat ttgcagtatt ttgtgttcaa
     1321 tgatgttacc accaatagtg cctataatct agaggcaaat aatatcaaca tcaagttcag
     1381 gtcaggcagg atactggatg tagccagtgc aagtgatcat ttgaaccttg ccgctttggc
     1441 tgataccgtg gtaaagcact atatttgcta cccgaaagaa acctgaccgg atggagttca
     1501 ctgcgcgcga attgtgtgat tttctacaag gcacattgga aggagacccc tccattaaag
     1561 tgcgcaaacc ggccagaata gaggagggac aacccggtga cgtgtgtttt ctcgcaaatc
     1621 ccaagtattt atcctttgct tatacaacgg ctgcttcggt gctcattgtc aatgccgatc
     1681 aggtgttcga acagcctgta aagtcaacac tgataagggt taaagatgca tataccgcta
     1741 ttgccaaagt gctcgaccaa tatcaaaata tgcaacgtga aagcgctgct gctaaccaca
     1801 aggttgagtc gaatgcaata gtttctcctt cagctaaaat tggcgccaaa gtgcatgttg
     1861 gcagtttcag tactatttct gaagatgtcg tggtaggtga taacagtatc attcacgccc
     1921 aggtttacct gggaaaacgt gtcaaaatcg gtgcgaatac ctgtattttc ccgggggtga
     1981 ttgttctgga tgaatgtgtg attggcgaca actgtatcat cggcgcggga acggtaatag
     2041 ggtcagatgg atttggcttc gccccacaag ctgatggaac ttataagaag gtagcgcaaa
     2101 caggaaatgt ggtgatagaa gatatggtgg agattggcgc aaatactact atcgacaggg
     2161 cgacaatggg ttctaccatt attcgtaaag gggctaaatt ggataacctc atccagatag
     2221 cccataacgt agagataggg gagaatacgg tgattgcagc tcaatccggc atctcaggta
     2281 gcaccaagat cggcaagaat tgcgtgattg gtggtcaggt gggcattgtt ggacatatta
     2341 ccattgcaga tggcaccaag attaatgcac aatccggtgt ttcaaaatct gttaaggagc
     2401 ccgatcaggc gcttacaggc tctcctgcat tcgaatacag tgcaggtatg cgcagtcagg
     2461 tcctgttccg caaattacct gaactgatgg atcgcatcaa acttctcgaa gaaaaaataa
     2521 gtcaattaga aaacaagcag taacgttata ctgcacgaca acatgaataa agaatatcaa
     2581 cataccatca aacagtccgt aacggtttcc ggtgttggat tacatacggg taaagaggtg
     2641 gatatgacct ttcatcctgc ggctgtaaac cacggatata aatttcgtcg catcgacctc
     2701 cccggttctc cggtaattga ggcagatgcc gacttagtaa cggacacggc tcgtggaacc
     2761 accattgcca aaaacggcgc cagtgtgagt actatcgaac acacacttgc tgccctggtt
     2821 gggatgggta tcgataatgt gatgatcgac ctcaatggtc cagaaacacc tatcatggat
     2881 ggttcatcga aagaatttat tgaagctatt cattcagtag gtgcagttga gcaggaggca
     2941 gaacgcgaat acatcaatat tgccgaaaat atcaccttca aagacgccaa tcgaaaaaca
     3001 gagatcacgg tgatgcctgc cgatgactat caggttacgg tgatgataga tttcgattca
     3061 aaagtcttag gtaaacaaca cgccacactt gaccacattt cagaattttc aggaaatatt
     3121 ggaaatagcc ggactttctg ttttctgcat gagttagaga tgttgtatga aaacaatctc
     3181 atcaaaggtg gcgacctgaa taatgccatt gttatcgttg ataaagaagt taccaatgat
     3241 gaaatggaga agctgaaaaa gatgtttaat aaagaatccg tttctgttaa aaaggaaggt
     3301 attctcaata accttgaatt acattggtcg aatgaacctg cacgtcacaa attactcgac
     3361 gttgttggcg atcttgcatt ggcaggaaaa ccaattaaag ggaaagtaat tgcaagtcgc
     3421 cccggccacc cgtcaaatgt tgcttttgca aaaaaactaa aagcctatta taaattgcga
     3481 cgcacacaaa tggttggtcc gcattatgat ccgaatgcag aaccgatact ggatattaat
     3541 tcaatatcga agatgttgcc acatcgctat cctttcttgc tggtagataa gattatcgag
     3601 atgggagaga atcatattgt cggattaaaa aacattacat tcaacgaggc attttttcaa
     3661 ggacattttc caaacaatcc tgttttaccc ggtgtattac aaattgaagc acttgcgcaa
     3721 acgggaggaa tatttgtatt gagtaaagtg ccggatcctg aaaattatga tacttatttt
     3781 ttgaagattg ataaagtaaa atttaagaga aaagtattac ccggtgatag tttgttgttg
     3841 aaaatggaat tgatggcacc tatcagaaga ggtatttgcg aaatgaaagc caccgcttac
     3901 gtaggaaata atatcgtaac cgaaggtgaa ttgacagcac aagtagtaaa gcgtaatcct
     3961 acagcatgaa tcagccatta gcctacgtac atccgcaagc acagatcgcc gacaatgtgg
     4021 tgatagaacc atttgcatcc atttcaaaaa atgtggtgat aggggaggga acctggatag
     4081 gtccgaacgt aaatattatg cccggtgcac gcatcggaaa aaactgtcgc atatttcccg
     4141 gagcagtaat ttctgcagtg ccacaagatt taaaatatcg cggagaagaa acagaagttg
     4201 tgattggtga taacactact attcgcgaat gtgtgaccat caacagagga accaagtatg
     4261 cattccgcac tgttgttggc agcaattgtt tgttaatggc atattcgcat gtggcgcatg
     4321 attgtgtgat cggaaataat gtgatactcg caaactcagt aaaccttgcc ggtcatattg
     4381 aaataggcga ttgggtaatt ttagaaggtc ttgtagcagt acagcaattt ataaaaatag
     4441 gggcacattc atttattgcc ggtggttcgt tggtgcggaa agatgttccg ccatttgtaa
     4501 aagcaggtcg cgagccgctg agctttgttg gtgtgaattc tgttggactg cgcagaagag
     4561 gatttgcagc tgaaacaatt gaacatatcc agaatattta tcgcttgcta tttgtgcgtg
     4621 gttacaacgt aacaaaggca ttacaatata tagaagcaga attgcctgcg tccgccgaac
     4681 gcgatatgat agttacattt atccgcaact ctaaggaagg tgtgatgaaa gggttccgcc
     4741 atattaatga atgattattt ccctcgaaaa tatttcaaag cgatttaatt acgagtggat
     4801 ttttcgtgat gtgaattttg cttttgatga aaatgatcgc tgcgccatta tcggaccaaa
     4861 cggatcagga aaaagtacat tattgaaagt aattgccgga caattaacgc caagcagcgg
     4921 caacataatt tatagcgata cacaagaaat tcctcaagaa aatgtttatc aaatggtcag
     4981 ctatgcagca ccatatttag agctcataga agaatttaca ctgtctgaaa tggtgcaatt
     5041 tcatttctct tttaaaaaat ggaagagcgg attgaatttg caggccatca tttcactcag
     5101 cggtcttgag aaacataagc acaaacaatt aagaacattt tcttcaggca tgcgtcagcg
     5161 ggtgaagctc gttttggcat tgtgcgccga aactccttta gtaattttag atgaacctac
     5221 caccaacctc gatgaatcgg gtgttgaatg gtatttgcat ttgattcggg attttggtgt
     5281 tggcagaatt atcctagttg gttccaatca ggaaagagag tataccttct gttcgaaatc
     5341 attaaatatt ctggatttca aaggttgaat ccaactttcg gattttacca ttttttaaca
     5401 attccttact tttctcatca ttttgtcatt tttcgtggtg taaatcttgc atggtatcaa
     5461 agccaattcc ctacatttat caccgaggag catcctgcat gtgtgggcgg cgaactcatt
     5521 gagaactaat tttttaatta aacacacttt atgcgaaaca ttgacacaac aaaagtgtcg
     5581 tggctgagga gcctgcgaca cttaatgttg gtcgtgtgta tgttagcagt agtgagtgta
     5641 aatgcacagg taagcgctta tggatttagc tcatttacag gcacattcac atagatcgac
     5701 ggcggtgccg gaacagtaac cgttgccact gcaaccgatg attcgaactt cggtacgtat
     5761 cccatcggat tcaatttcac atttaatgga acggtgtata ccacttttgg tattcaggca
     5821 aacggtttca ttagtatggg ggctgttcct tcaagcagtt atactgctat ttcttcaggt
     5881 tcatccaaca acattatctc tgcctttaat gccgaccttg aaggggatgc agttgcagct
     5941 ccgggagcaa agctgatgta tcagcttagt ggtgcggcag gaagtcaggt gatgactgtt
     6001 gaatggaaaa actggggatt ctggagtgca ggtttgaatg aatgttcatt ccaaattaaa
     6061 atgtatgaaa catccaacaa tgttgaaatt gtgtatggtt cagctccggg aacaaccagt
     6121 aaaaccttac aagtaggttt gcgtggtgca tcttctgctg attataacaa cagaacaacc
     6181 acaacaaact ggtcggctac aactgcagga gctagcaata gcgcctcttg tacttttagc
     6241 tctacagtga aaccctcttc aggtttaacg tttcaatgga caccgccgac accatgtaca
     6301 ggcactccaa ctccgggtac cattccggcg actgcagctg tatgtagtgg tgcagccacc
     6361 ggcgtaacac tcactgctac aggctataca acaggtgtat ccggtattgc ttttcaatgg
     6421 gaagaatctg atgacaatgg tgttacggat acttgggcaa atgcagttgg tggaacagga
     6481 gcaactacgg cttcatatac cacaccggca ttggcatcta ctcgttatta tcgtttaaaa
     6541 gtaacttgta caccaagtac cttatttgat tacaccggtt cttgcatggt atctgtaacc
     6601 aactgtacat tcgatgtaac ttatacaacc ggaacattat ttacttctat tatgcctgca
     6661 gcaggtggca caggttctgc aatgcccggc tggttaggaa cttcaggtga tgataataca
     6721 accactacag ttgaccttac aggaacttca ttcacctacc aaggagcacc tgtatccgga
     6781 ttccgtgctt gtactaacgg ttggatgtca ttcaacacag ctactacaac cacaaccttc
     6841 tcaaattcat tgtcttcaac ttcacagtca aaggtattgg ctcctttctg ggatgacctt
     6901 gtattgacag gaaataatta tgctaaccgc gacaactgta tgcgttataa aattgacggt
     6961 acgttgggaa gcggcacggc tgtaattaca atggagtggg ccggtatgga gcgtttcagc
     7021 aaccccggac caaactggaa tttccaggtg aaattgtatg aagcaggtaa ctatattgaa
     7081 tatatctatg gaacctttga aggatttgat ggtaccaact atacttcaca tacttattca
     7141 attggttata atggttctaa ccctgccggt accggagcag cagatcgctt tgcattgcaa
     7201 accgttctta ctaaccattt ctccagcgcc tctgatcccg catctcatgc agtaaaccct
     7261 tactgtttct ctaaatacac tttaactccg ggaacataca caggtccggt aacggcacct
     7321 gctattcctg ttccaactaa caacgaatct gccggtgcat atacactcgc agtgggaaca
     7381 gccgaagtaa catcaccaaa cttctgcagt acttattata cttcacgtgg tggtacaaac
     7441 tcaggaatgg gtgccgctcc atgtgctact gcaggtaatg aagatgatga tgtttggttt
     7501 aaattcgtaa ctacaggagt aaccgattat aatattcgtg ttcgcccttc accgggatat
     7561 gatgcagttg ttcaattata tgatgcttca tttacatcaa ttggttgtgc aaatgctgta
     7621 ggcgcagcaa cgtctacaac tgcaggaact gaaactatcg ctgccacagg acttactgcg
     7681 ggtggaacta cttattatgt gcgcgtttac gctaactcaa taggttccgg aacaagcggt
     7741 gaattttcaa taaacgttta ccaatctaca ccaattccgg ctaacgacga ttgcgcaggt
     7801 gcattcacat tggcagttgg aacatcttgt agctatactt caggcacttc tttaactgct
     7861 acatcttctg caggtgaagt tgctgcttgt accggcacag ctgatgatga tgtttggtat
     7921 aaatttgttg caacggctac taatcctgtt atcactgttc aatcaggtgc ggctttcaat
     7981 gctgtattat ctgtttacag cggaacttgt ggtgcacttg ctaatattta ttgtatcaac
     8041 gccacttcaa ctgcaggtat tgaaactgta aatacaacag gacttgtaat tggacagaca
     8101 tactatattc gtatttatca tagcgcagcc ggtgctggtt caggatcatt ccaggtttgt
     8161 gtttacaatc ctgttccttc ttgtactact tatatcagcc ctgctgatgc agcagttggt
     8221 attgttggtg cgggaactac attacaatgg aatgcggcaa catatgctac aagctatgat
     8281 gtgtatttcg gcactgtaac gccacctcct tttgctacaa atacagcatc aacatcttgg
     8341 tctacaggtg cacttgcacc attgactact tattactggt ctatcgttcc taaaaacgat
     8401 ctcggtgatc cgggttcatg ctcaatttat agcttcacat ctgttcctga atgcacaggc
     8461 actcctgtag gaggcgctgc agcttcaacc gctgcttctg tgtgcggaac cggaacttct
     8521 tttacgttat cactaaccgg tgcatcaact gatgcaggta ttgcctacca atggcaagct
     8581 gatgcaggtg caggatatgc tgatataatc ggtgctacaa gttcaacata ttcaactact
     8641 gaagcaggta ctaccagcta tcagtgtgtg gttacttgta ctccatcagg cttctctgca
     8701 acttcaactc cggtttcagt tacatttaat ccggctccaa ccggcgatgt gcttgctgat
     8761 ccaattatca tagcttcatt gccgtatact gcaacaggaa ataacctgtc atctaactgt
     8821 tggactaacc aaatcggtca gtcttcacct gatgtgtatt atcgtgtaat tcctgaatgt
     8881 agcggaactt tgaacttgaa cctttgttca ggtggtacga tctctgatac ctatattcat
     8941 atcgcagatg caacaggtac tatcctcgca tctaacgatg ataacggtcc atcatgtgca
     9001 ggtttattat catcaattaa ctatgctgtt tcaggtggtg taacgtatta cattattgta
     9061 gaaggtttta gtgcaaatga aggtacttat acattaaacg tatctaattc tgcgacatat
     9121 tctgtttggt atgcagatgc agatagcgat ttgtttggtg atgccttctc aacaacaaca
     9181 acttgcgatg gttttgcacc atcaggttat gttggaaata acctcgattg taacgatgct
     9241 gatgcagcag taaatccttc tgcaattgaa gtatgtaacg gttatgacga tgagtgcgac
     9301 ggcttgacag atgacgctga tcctgatgtg atcggattct ctgtatggtt tgctgatgca
     9361 gatggcgata cttatggtaa cgctgcaatc attgcagaag catgtgccgc tcctgttggt
     9421 tataccgctg atccttcaga ttgcgatgat actaacagtg caatcaatcc gggtgctacc
     9481 gaaatttgta acggtggtat cgatgataac tgcgatggtt tggcagatga tgctgatcca
     9541 ggagtagttg gcgctccttc atggtatgta gatgccgacg gtgatacttg gggtgatgca
     9601 gctacagctg tatctgcatg tattgctccg ggaggttata tagccgatgg atctgattgc
     9661 gacgatactc aaatattgta tactgatgcc gatggtgatg gtcacggaac tgctacacct
     9721 gctccatgcg gtgcaacaaa taccgacgat tgcgatgata gccaaagcac agtatatccg
     9781 ggtgcaccag aattgtgcga cggtcttgat aacgattgcg atgctactat cgacgaagga
     9841 acagttactg caaccgtgac acctgctgtt ggtgctgtaa catgtaaaac atatccttat
     9901 acattcagcg caaataccgg tgttggctat acttatcagt ggtttaaaaa tggtaacctt
     9961 gtagttggtg ctacaggtcc aaccttagct actgataaac cgggttacta tcaagtacaa
    10021 gtaaacgttc ccggcggatg ttttgcaatg tctgacgcca gcttgctgac cgttaaccca
    10081 ttgccgaacg ctactatcag cgcgccaaat ggcactagcc tttgtgcagt tgtgaaattg
    10141 aaagtaagcg aaaatgcaac atggacttac caatggtatt tagaaggtac tccgatagcc
    10201 ggcgctacca acttcttata ctatccatct gttgcaggta actactatgc aattgtaaca
    10261 agtgctacag gttgtttcag acaatcagct actttaacag tgacttcatg tcgcgatgct
    10321 gaagtttccg gaaatgaaac aatgaatgta tatccaaatc caacttcatc tatgtttaac
    10381 attgatttga tgatggctga tggatataat ggtactgctg atgtttacgt tacaaacgtg
    10441 cttggcgata tcgttctcaa caaacaactc actgtaaaca atggtgccgt ttctgccgaa
    10501 ctcaaccttg gtgttgatgc agctgcaggt atgtatcttg ttaaggtggt tgcaggtgga
    10561 aaacaattca accaacaact cacagtagtg aaataattca ctcttaccct tcaataaaaa
    10621 acgaccattg ggaaaccgat ggtcgttttt tttgttatta ttgccaatat gaagctgaca
    10681 aaattgttta ccgaattctt tgaaagtgag cgtacaggcg gtatcattct gatttgctgc
    10741 acatttgttt cattgctcat agctaattct gcactcggaa ccggctataa tgctttttgg
    10801 catcatgaaa ttgcgggcca ttctattgta cattggataa atgacctgct tatgacattt
    10861 ttctttctac tggtgggatt ggaaattgaa cgtgaaatat atatcggcga actcaaagag
    10921 tttcgaaagg cttcacttcc aatatttggc gcaatagggg ggatggcaat accggcgctg
    10981 gtgtatatcg gtattaatat ttcttctgat aatatgaatg gattcgggat cccgatggct
    11041 actgatattg catttgccat tggaatttta tcattgctcg gaaatcgggt gccggcatcc
    11101 ttaaaaatat tccttaccgc cctggcaatt atcgatgatc tcggagcaat tattgttatt
    11161 gctatttttt actctcaatc cgtgagtctg ctttatttag gagctgcact taccctattt
    11221 atgataatga tcattatgaa caggatgaaa gtaatgcgcc tgtatttata tctaataccg
    11281 ggaatattgt tatggtactg tatgctccag agtggcattc acgcaactat tgcaggtgtg
    11341 ttgatcgctt ttgctattcc cttcagagaa ggcgataatc attctccaag ctataaactg
    11401 caacattttt tgcacaagcc ggtagcgttt tttgtgcttc cgatttttgc acttgcaaac
    11461 acagcgatag tattaaatac acaattgttt tccggccttc aaagtacaca tgcattgggt
    11521 attgctttcg gtttaatttt tggaaaacct attggcatat tgttgatgtg ctatttagca
    11581 gtcaaactaa aaattgcagg attacccgaa ggtgtgaatt ggaaacaatt agcaggtgtt
    11641 ggtttactcg caggtattgg atttacaatg agtatattta tcagcctcct ggcatttgaa
    11701 gatgatcaaa tgatcaatat atcgaaactg gcgattttaa tatcttctat tattgccgca
    11761 gttacaggat tatggtattt gagcaggcaa atggataaac aaatttctgc ataaaaaaaa
    11821 cagcaaccat ccctttcgag acgcttgctg cttgtttatc ccttaaaaac aggtcaaata
    11881 tacatcgacc ggtcaaattt tgcaacttct aaagcaatat ttttaagcgc ttagaagcga
    11941 tttaacattg aagtttttcc tctcaattaa aggatatgcg ttgcatcgta atggtataag
    12001 cgggtaatat ccttgttggt tataatgcct accaaatcgc cacttttaac tacaggaact
    12061 gcttgaaatt tgccggctgc caatatttcc agaatgtctt tttgtggggt atcgggggat
    12121 acagtggtag ggttggcagt catgaccttt tcgatggaga atgcggcatt taaagcggct
    12181 ttagaaacat caccggaacc ggcaagataa tcttgcatga aagtaagcat atcctttatg
    12241 ctgatgatgc caatcaatcg tgtcccttca gcaacaggta gatgctggat cttgtgccgg
    12301 gtgaaaaatt ccattacctg atcgaatgtg ttattcacac cggctacgat cacattgccg
    12361 gtcataaagt ctgaagcgtt gcgtttatag atcataagat aaaaatttac tctgcaaacc
    12421 tactaatgtg tcatatggct aaaaatgatt ttcgtcaccg tttaaggtgc cttacgtcaa
    12481 aaaaaaagcg gcatctcaaa tgaaatgccg cttaatttgt tgcttgatga tatcttactg
    12541 cccgccttta agatagctat agtgctttcc gtaatacagc atttgttgcg tgaagtcttc
    12601 aggatagctt accgttacat cagttacctc tccctgatcg tttttcacta tcgacaactg
    12661 cggattgata aagccccaat agggtggtgt tttaatatta gcagtgcgtg ccaatacttc
    12721 ggcatgtatt tcttcattca cctgaacacc gaaagtttct accaatttca ttcctgcttc
    12781 gtaatcgcct tcgcttttaa tgcgttgcac ctctctcaat aattctccga ataattggcg
    12841 taatgcttcg taatcattga tcacaaaata ggttttatca ttttcatatt tgcgttcaat
    12901 cacatttttc gacttccctt tttcaaaaac ccaggctgca atcatctgac gattgcgcat
    12961 atgcgattct tcaattgttt tgccgggctc aatacggcga agttgtaaca tcaaaccatt
    13021 gcggatatag ctgtcgtatt ctgctttccc tacttccagt gaaggaatca atccccattc
    13081 aactaattga ggatccatgg tgtaatacaa tgcaacaagg tccgctctcg cttcctctaa
    13141 agtgttggca taattcttca gtgtttcttt aggtgttcca accccgggat tgatttttcc
    13201 gctcgcatga ccgataacct catgcatcgc tgtatgcatt tttgctgcca atgaaccgta
    13261 atttaccgcg cgttgtcttt cctcttcggt ataacaaaat tcactgagta aacttgaatt
    13321 tcccgcttgc tcatatgcat aaacaatatt tcccaagctc actgatttgc tgccgtaatc
    13381 tgcgcgtatc caatttgaat tcggcaagtt cacaccaata ggcgttgatg gcgatgcatc
    13441 tcctgcttca ccggcaacat tgacaactcg gtaggaaacg cctactacgt tgtctttttt
    13501 atgcgatttc attatcggtg aataatcctc aaaccattga gcatgctcag ctaatttttg
    13561 catgcgttct gaagcttcga aatccttaat ctctacaatg ctctcaaatg agccgcgata
    13621 ccccatcggg tcattgtata cctcaatgaa actgttgatg taatcaatgt ctccgtgcgt
    13681 atcctgcaac caggcaatgt tatattcatc ccaaactttt aaatcgccgg tttcataata
    13741 tttgatcagt aatcgcaatg catttgcctg catgtcattt tcacaatgtg gcatggcgag
    13801 ttttaagttt gcgatgatct gcttaatggc atcaccatac aatccatctg ctttatacac
    13861 cacttcaacc aatcctttat ctgtcttgat catgcgactg ttcaatccat gagaaatcgc
    13921 cttttcatcg ttcatgccgg gctgcgcagc ataatacgcc tctacttctt tttctgatac
    13981 atcatctgca taaaagttcg aagctgaatt caatagtaaa tccttatcag gttgcagata
    14041 tacctttttg ttatctacaa caggatcaaa catgatgtcg attattttag tatctaattg
    14101 agcacctgtt gtttttacaa gcgaaataaa atatgccttt gaacatccgg gcataatctt
    14161 gtcggtagaa taatgatgat gaatgccatt gctgaaccaa acacgtttcg tgtaaatcat
    14221 aaaatcctgc cattctttcg attgcttatc acctgtataa ttctctacaa tatgctctaa
    14281 tgctttgcga atggtaaggt tgtatttata gttttgatcc cagataatat cccttcccgc
    14341 ataacctgct tcggtcaaat agtagactaa cagcttttgg ttaagtgtta atttatcaaa
    14401 atcaggaatt gtatatctta gtatgcccaa atcagcaaat tgttctgtat tccaaacaaa
    14461 gctgtcaccc attctaggtt catcagcgct attcacggaa tattgctgac tcgttcctgt
    14521 aacttgttgt aatccttgtg cctgtgcagt gaagcatata acacctccaa acagcaaggt
    14581 catgattgat ttattcattt gattagtgtt gagaatacaa aattgcataa atcgggccga
    14641 atttcatata tccacagccc gatacttgca tgttaatatg tggcatagta tatttacata
    14701 aaattacgcg tatgaagaag aaacttttgc tttcgatttt agtagtaatg gcaggaacta
    14761 tcgctgctgt ttatgctgta aatcatcgtt ctgaagctcc aacacagatt gctgctccaa
    14821 cagaacaagt aagtgctgaa aatcctgcac attgcgatcc ttctaactgc tctcctgaag
    14881 aagcagcaaa atgtccatat gctaacaaag cagcacaggc aactacagaa tctgctgata
    14941 atgtatgccc tgctactaaa gaatgcccac cgtcgaaatg taattccgcc tcaaaaacgg
    15001 ctaccttata atctcttaaa aacagatttc caatggcaaa gaaaccacgg cgaccaccgt
    15061 ggttttttgt ttgtacatat ctgcactaac ctttatcacc cgttttggga atgttaatca
    15121 ttctttaaac ggtaattccg atatctattt gtatcgtaaa tcacgatgtc atgcacaacg
    15181 aacatgataa cgtgacatga ataaaatgtg ataatccggt ggtcgatatg tttgtatgat
    15241 cacctcaaag atggttacag atgttaaatg ccatctgttt gcctgttata tgtttcatgg
    15301 gttttgaatg aacgccgata gtaatgtcgg cgtttatttt ttcatccatt atccttccat
    15361 cgccacaaat gcatacacgc ataagtgcga tatggacgcc actgctcggc aattttttcc
    15421 atgcgcaatc gaaatttctt tttgtctctg ggccgcaatc cataagcacc aatcattgct
    15481 tgttgaatgc caaggtcatc cgttgcaaat acatcttttc ttcccaaggc aaacatcaat
    15541 aacatttcta cagtccatct gccaacacct tttatttgag tgagatattt gatgatctcc
    15601 atatcatcca tcgcctgtaa ttgcttaacc tccatgcctt gcgtttgtgc aaaggtggct
    15661 acgttgatca catagttagc ctttgcctga cttaaaccca ttccccgaaa tgtttcaatg
    15721 gacgttgcca tgattttttc tgccgtaggt ttgccgtcat ataacttatc gaaacgcgca
    15781 tgaaatacat cggctacctt ggtggatagt tgctgactca tgatagagta acacaaatag
    15841 gtgcagatat ctttttgctt cttcaaagta aaggctccat gctttttaat catcgccgcc
    15901 attaccgggt ccttcgataa atgttctaca tattccatgc ggtaaatatc atgaaaattg
    15961 aattattcag cagccctgac tattttcaca ccatgtctgc cacagagagt aataccatca
    16021 gttctaaaat gtggttctgg tcgcattttg cccttgtagt aattgcctgg atcggacctt
    16081 ttattttcga ttggtatctg atggtaacag gatatgcgct ggtggtaatt caatttgccg
    16141 tgttcaaccg ctgcctgatg aatgacaaac acgacctggc ggaggaaggc gacatgacgt
    16201 tttatgccca actcctcgaa tctataggat ttaacttctc aagagcaaaa gttaagcgta
    16261 ttgtccgtcc gtggatatat gtttttctgg ctgttttcgt catcttcctc caagtgattt
    16321 tcagctatgt ccccgtttta catattccgg gataaatccg catcacaaca ccataatctc
    16381 tcttatcttc gcagcaaata aactgttatg aagaagatct tcattttggg agcatgcgga
    16441 ttgttccttt caatagccgg tgcccaaggc gtcatgacac ccgaattatt gtggagcctg
    16501 ggaagggtag gagcggaaac tgttactccc gatggtcaac atgtaatatt cggcgtgaca
    16561 tattacgaca tcgccacagg ggtaggggag cgcaacctgt ttagtatagg ccttaccgac
    16621 cataaaccca ttcaactgac cactacaaaa ggcggtgaaa atagtgtgat gacacttccg
    16681 ggaggtaaaa tgggctatat ctatcagggg caaatatggc agtcggaatg ggatggctct
    16741 aatgccaagc aacttactaa ttatcctgat ggcttgtcaa atgtgcggtt ttctcctgat
    16801 ggaaagcata tcttgttttc aaaggatgta aaactgatcg atgtactggc cagcgaaaaa
    16861 tataaggatc tgccaaacag ttcagttaga atatatgaat cgctcaacta tcgtcactgg
    16921 gatacatggg aggatggcgc ttacagtcac atcatgcttg ccacctggga taatggcatc
    16981 ataggtcctg aaaaagatat catggatgga ttgccgttcg atagcccgca aatgcctttt
    17041 ggcggtgtgg aagacttcat gtttcacaac gataacgatc atatcatcta tgtgacgaaa
    17101 gcattacacg gcaccgatta cacccagagc accaatacgg atatctacct ctataccatg
    17161 tctaccggcg ataccaagaa tattacagcc ggcatggaag gctatgatac aaatccaaat
    17221 ttttcacccg atggcaatcg cctggcttgg ctgagtatgg ctcgaaatgg ttacgaggct
    17281 gacaaaaacc gactttatat caacgatatt aaaaataagg tggtgatcga tgcttcagaa
    17341 tcatttgatg aatctattga agctcttcgc tggagcaata atggtaattt tatctacttc
    17401 ctcagcccta tcaacggaac tttacagttg tttgagctcg atgtaacaaa cgttttcact
    17461 aaggttgatg gtcgcaaacg caatcccatc cgccaggtaa cttccggcga ttatgacgtg
    17521 gccagcatcg ttggcgaaac taatggcatg ctcgtggtaa cgagaaccga catgaatcat
    17581 gcggccgaat tgttccttgt gaacccctcc accggcgtta tgaccgcaat cacggagatc
    17641 aacaaaccga attacgataa aatcggtatg tgcaaaaccg agcgcaaaat ggtaaaaacc
    17701 accgacggac tcgatatgct tgtttggttc atttatcctc cgaatttcga tcctaataag
    17761 aagtatccaa ccttattata ctgtcaaggc ggaccacaaa gtgcactcac gcaattttac
    17821 agtttcagat ggaactttca gttgatggct tctcagggat atattgtggt ggcaccaaat
    17881 cgccgcggta tgcccggaca tggcactaaa tggaatgaac aaatcagtaa agattggggc
    17941 ggacaggcaa ttaatgacta ttacagcgca atagattacg ctaaaacgct tccttatgtg
    18001 gat
//
LOCUS       DAOQHY010000058        17912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1458_length_17912_cov_5.784790, whole genome shotgun sequence.
ACCESSION   DAOQHY010000058 DAOQHY010000000
VERSION     DAOQHY010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 17912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17912
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1458_length_17912_cov_5.784790"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1706
                     /locus_tag="PLL28_06460"
     CDS             <1..1706
                     /locus_tag="PLL28_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNF69000.1"
                     /translation="IQLACKAEDTTDMKNSFIHLHDALSTFENHYGPFRWDRVGYAVV
                     PFSGGAMEHAMNIAYPLFAVNGTTLWENLYAHELSHHWWGDLTTCSTPQEMWMNEGWA
                     VFSEHLFQESLYGEQAYMDAVKANQLDVLHNAAPRDGNNYFAISGVPESYTYGATTYN
                     KGASVIHTLRGYMGDDAFFSCITSFMETYQFQPVSAANLRDHLTACSGMDMTDFFDDW
                     VYQPGSLAFDVLSTGGNKGTITCLHIQQYLSHATHYGNNVPLTAYFFDYNFNLFDSVD
                     IVMSGDRQLTTIEVPNPGSIVYTILDYRNKLNDAVTSDEVHFTEPGTYSLDNAMMDIE
                     ITSVTYPGFIYAQHFWAAPNNYIAAHPGLHVSTDRFWRITTNIPVTGDATAKFLYNGQ
                     ATIGSGYLDNDFITNSEDSLVLLYRNGPQGDWIIYPDYTLNTWGSANDKRGAFELSAV
                     TSGDFCIGIYDASIPDQPLYIPETDCAFNGAIENLSDNSLTVYPIPANQLIVATVDQQ
                     LMGMQLSLYDTSMRKIWTGRIDNLSMSIPTQNLPAGTYQLCVENARSERIAAEKVLIM
                     H"
     gene            1730..2617
                     /locus_tag="PLL28_06465"
     CDS             1730..2617
                     /locus_tag="PLL28_06465"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HNF69001.1"
                     /translation="MKNVTVIGAGTMGNGIAHVFAMKGHKVTLVDVSEPALQKAVATI
                     TNNLDRMVAKEKITADEKAATLSNITTYANFDHSTNADLVVEAATERVDLKLKIFTEL
                     DQICAAHTILASNTSSISITKIAAATKRADRVIGMHFMNPVPVMKLVEVIRGYATSDE
                     TAKTIMELSAALDKVPVEVNDYPGFVANRILMPMINEAIYTLYEGVAGVYEIDTVMKL
                     GMAHPMGPLQLADFIGLDVCLSILNVLHDGFGNPKYAPCPLLVNMVTAGYLGAKSGEG
                     FYAYTAGNKDLVVSPRFNK"
     gene            2690..3619
                     /locus_tag="PLL28_06470"
     CDS             2690..3619
                     /locus_tag="PLL28_06470"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HNF69002.1"
                     /translation="MTPVFAKAYSPSEVHFQIEDCDNRNEPRKVMMCTPDYYNVVDVK
                     NVYMQHQKGNINKERANIQWDNIRNSYLALKNQKFLDDVVVINGVEGMEDMVFCANQS
                     FPWVMWNGEKIAIMSRMRHENRQNEIPYIEDFYQDHGYKIVELQSKYHFEGMGDLIAH
                     PGKRVLYGAYGFRSEKAVYDEISRIVDAPIITLELINEHHYHLDTCFLPLNHTEVLIA
                     PDAFTPESLAIIRRMFEGVYEIPEDEAIDGFACNAHIIYGTNKNSSAAVIQRGNPVSL
                     NIIQQSANPVIETDTTEFMKSGGSVFCLKMMFY"
     gene            3892..4524
                     /gene="bioD"
                     /locus_tag="PLL28_06475"
     CDS             3892..4524
                     /gene="bioD"
                     /locus_tag="PLL28_06475"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HNF69003.1"
                     /translation="MKKIFITGIGTDIGKTVVSAVVVEALFADYWKPVQSGGLDYTDT
                     QRVQNLISNPKSQFHKEAFLLKQPMSPHAAAALDGVEIDLDNIVVPETSNTLVIEGAG
                     GLMVPINNSTLMLDMIAKWEASVIVVSKNYLGSINHTLLTCAVLRAKQIPVAGIIFNG
                     MENVSSEQFITQYSDIPVIGRVAHTDVLDKPFIKKHAEFIRKRMQQLKIS"
     gene            4526..5821
                     /locus_tag="PLL28_06480"
     CDS             4526..5821
                     /locus_tag="PLL28_06480"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HNF69004.1"
                     /translation="MRDDGPIWYPFTQMQLAPEPVQIVSGKGALLFTADGNTIIDAVS
                     SWWTNIHGHAHPYIRQKVHEQFGQLEHVIFAGFTHAPAMHLASRVTAMCGSDYTKMFF
                     SDNGSTAVEVALKMALQYHQNLGAKKHRIIALEGSYHGDTFGSMSVSARGAFTAAFHD
                     KLFDVTYITPPLPGNEDICMQELEAALSEGDVAAFIYEPLIMGAGGMLMYTGMALDTM
                     IYTAKQHGVLCIADEVMTGFYRTGKFLAGHYANHQPDIICLSKGITGGSMALGVTICT
                     QAIFMAFYSEDKMKALYHGHSYTANPLACAAANASVDLCLQPGFDHALTTIASRHQHF
                     TAALQVYSHSDIPVVKNIRQCGTVLAMDFVTSNDTGYFNSIRDMLYHFFMKRGVLLRP
                     LGNTIYIMPPYCITNEALDEVYNAILDALALLAEGKTLQ"
     gene            complement(5822..6604)
                     /locus_tag="PLL28_06485"
     CDS             complement(5822..6604)
                     /locus_tag="PLL28_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNF69005.1"
                     /translation="MTYSTILYTIADKVGTITLNRPDKRNAFNADVVAELTEAFTDAQ
                     ENESVKVIVLAASGEVFSAGADLGYLQSLQQNSFEENVADSQKLMALYKLMYTLSKPI
                     IAKIEGHAIAGGCGLASVCDFSFAVPEAQFGYTEVKIGFIPAIVMVFLLRKINERHAK
                     ELLLTGKLITAETAEKYGLINAVIPKNKIDEAVSGLCVELIAQTSRVAVATTKSMIAA
                     VQEMGLDEALQYAAEMNAKARATDDCQYGIRSFLEKKKPEWK"
     gene            complement(6608..9004)
                     /locus_tag="PLL28_06490"
     CDS             complement(6608..9004)
                     /locus_tag="PLL28_06490"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF69006.1"
                     /translation="MRNFLLICLLLSTISLTAQSPGQTVRGTITDAASKYPLIGATVL
                     LYADSTMLGGATTDEQGDYRITNAPVGRVTLRFTYVGYEDITMSNVIVTGGKEVIINV
                     ELEEGAEALDAVVVTAKSDKGEVNNEMAINSVRTFSVEETDRYAGSRGDPARMASNFA
                     GVQGADDSRNDIVVRGNSPIGVLWRLEGIDIPNPSHFAIAGTTGGPVSIINNKYLSNS
                     DFFTGAFPAEYGNAIAGVFDLNMRRGNDEQHEFSGQLGFLGTELFAEGPINKSKGSSY
                     LASYRYSTLQLFQFMHIPIGTTAVPNYMDGAFKLSFPKGNAMWNVFAIGGKSKIDILV
                     SEYTKEETELYGDSDRDQYFGTAMSVAGVSYARQYNPSAYGKWVAAYSVQENHANHAF
                     VFRDVNFDIDSIVPNMNYSFLDQKASLIYSLNKKVNSRHSFKTGASFESIWYNHIDSN
                     RNVYTWTWDNRYDFEGNNYLAQAYFQWKYKYNERLLFTGGLHAQIFTINSNSKSLEPR
                     AGMQWRVDDRSSLSLGLGLHSQMLPSYQYTYMHYQADGSALLINEDVGFIRSAHMILG
                     YDRKLSDNLRVKSEVYYQYLYDVPVEVAPSAFSLANEGSGFSRFFPDSLQNTGTGKNY
                     GAELTIEKFFSNHFFFLITGSLYDSRYVGSDGIERKTDFNGTYAGNMLGGYEFTISDR
                     NVLTVGIKTTYAGGKLTTPVDTAATIAAKELIYIDSLTNTIQLKDYFRFDVKLAYRIN
                     SGKRSVTHEIALDLVNVLNIQNVLGLTYAPNPADPTASPVKEEYQLGFLPLFYYKIDF
                     "
     gene            complement(9213..9764)
                     /locus_tag="PLL28_06495"
     CDS             complement(9213..9764)
                     /locus_tag="PLL28_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69007.1"
                     /translation="MKKGFLVILAIASMATLNSCGKKKGPSAETKQAMATFESEWKAL
                     GDQIAAWETTMNTAMDGMTQMMTATRSMDITKLSGESKSMAEMNLMMCSKIDEQMVAM
                     KTGFAKAKTDFAAATDEYGAWKKKGQEEHLDDATITSELANWTTKLTEWKTTVEGLNQ
                     QLSGMQDACKQSCDAITAMVPAK"
     gene            complement(9811..12420)
                     /locus_tag="PLL28_06500"
     CDS             complement(9811..12420)
                     /locus_tag="PLL28_06500"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNF69008.1"
                     /translation="MEISKTYDPASIEKKWYAHWLANRYFHSKPDNREPFTIVIPPPN
                     VTGVLHMGHVLNNTIQDVLIRRARMQGKNACWVPGTDHASIATEAKVVKMLREQGIKK
                     SSLSREDFLKHAWEWKEKYGGIILQQLKELGCSCDWDRTRFTMDDLYYDDVIDTFIDL
                     YNKGYIYRGLRMVNWDPEARTALSNEEVIYQDVTSRLHYVKYQIKGDNVYLTVATTRP
                     ETILGDTAICVHPDDERYKEFIGKTVLVPLIKREIPVIADTYVDMEFGTGALKITPAH
                     DPNDYEIGLRYNLPVIDIMNEDGTLSEKAEILIGEDRMVARKKMVAMLEEEGYLVKIE
                     ELKNSVGFSERTNAMIEPRLSMQWFISMKEISKPALEHVMNGDINLHPEKFINTYRHW
                     MENVKDWCISRQLWWGQRIPAWYNASGEFVVAKTEAEARAIFTSKGMSLDGFRQDEDV
                     LDTWASSWLWPISVFNGMKDPKSADIEYYYPTQVLVTAPEILFFWVARMIIAGYEYRN
                     EKPFSDVYLTGIVRDKLRRKMSKSLGNSPDPLDLIAQYGADAIRMGILLCSPAGNDII
                     YDDKLIEQGRNFNNKLWNVLRLIKGWEVTETSQTKNEAAIQWFDSKLNDTINTINDHF
                     EQFRISDALMCLYNFIWDDFCSWYLEFIKPEFGQPIDSITYEKTIDFFEGCLKLLHPY
                     MPFITEEIWHLLRDRKAGDDIVITQLNDAGNINNNILASGNEAKELISRIRDIRAKNN
                     VSPKVAASLYAEGDITSMQAFRAVIMKMANLEVFESVTGDVKNAVTFLVGTQKFFVVV
                     QMEINIEDERKKLEEQIAYQKGFLLSIDKKLSNEKFVGSAPAAVVDLERRKKADAEEK
                     IRILEESLRNLGN"
     gene            12441..13733
                     /locus_tag="PLL28_06505"
     CDS             12441..13733
                     /locus_tag="PLL28_06505"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNF69009.1"
                     /translation="MEGGISPRFRIFVHVKKAYLLVLVAIASVFQAEAQMQAHDTCRF
                     MTYNVLNFDYTGNFKENYLRTIVSEIHPDIMVVQELIDDSGAARFRDSVMLQVDTNYA
                     MAEFINSYDTDNGLYYRKDKYAFLSNTVVTTELRDINQFEMRHIASGIDFYVYSLHLK
                     ASSGGTNAAQRQREVDSLRKATLALPDTAHILVCGDFNIYGSYEEAYLHLLDPTDPGY
                     VIDIWQDSLTSTWNNIANTDYLTQSTRIAAFGGGSTGGMDDRFDMILMSPSLVDTAGM
                     GYIPGSMYPVGNDGNRFDNEINDPVNTSVSEEMADALYFTSDHIPMFAEFSFSYIIPE
                     EPEDTTDTLIQQIAETDLLLQLYPNPAGDVITATSNFNAEGYIIYGADGKTVYKMTEK
                     SIPAGISEKIAIADLAPGLYVFVSFNGIKRVQSTFLKN"
     gene            13776..14987
                     /locus_tag="PLL28_06510"
     CDS             13776..14987
                     /locus_tag="PLL28_06510"
                     /inference="COORDINATES: protein motif:HMM:NF017072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNF69010.1"
                     /translation="MQQFKVGIIREAKFPPDARVPLIPAQVQQLRQQFPNIEIVVQPS
                     KGRCYADYEYTDLDVTLQEDLHDCDILFGVKEVPYDLLIPGKTYFFFSHTIKKQPHNK
                     KLLQVLMQKNVRMIDYECLKNTEGNRVIAFGRWAGIIGAHNAIWTYAQRLGEQHLKRA
                     RDCRDYYELKYQYRGLTMPVIKMIVTGTGRVGKGAIEFLRKIGVREVTKEALLNETFS
                     EAVFCVLDSDDLYSRKSDGKYDRNEFHSHPELYHCTFSPYTKVCDVLINCIFWNPAAP
                     SLFHKNEMDATDWKIKVIADVSCDVKGGIPATIRSTTINEPVFGYDVATDMEIAPYIP
                     ESIDIMAIDNLPNELPRSASEEFGQQLISTVWNELFQDNSRMLDEATICKNGNLNKHF
                     EYLRDYVTGES"
     gene            complement(15142..15744)
                     /locus_tag="PLL28_06515"
     CDS             complement(15142..15744)
                     /locus_tag="PLL28_06515"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNF69011.1"
                     /translation="MGLNDNLSDRAKEDLALVESARRGDQRAYGELMSRYKDTIHYMV
                     LKMVHNRDDAEDLTIEAFGKAFLHLERYTPDFAFSTWLFKIAVNNAIDFIRKKRLHTL
                     SLDDEQDEFTRSAFTNIRTNNPDPEEKFIKEQRANIMRTILDELNPKYKALVEMRYFD
                     ELSYEEISETLNIPLGTVKAQLFRARNLLAEILADRRAKM"
     gene            complement(15735..16862)
                     /locus_tag="PLL28_06520"
     CDS             complement(15735..16862)
                     /locus_tag="PLL28_06520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF69012.1"
                     /translation="MGDSIILDSIFYLFCGAAAVQLFYILFFYLRLARYQQKSVMQDH
                     ALPPVSIIICARNEDSNLKKNLPRILSQDYPEFEVIVVDDNSEDSTTEYLFFLSQKEP
                     RLKRTSTRNSNRMMAGKKFPLTLGLKAASHEIVLLTDADCYPASDKWLREMMNGYGSG
                     AEIVLGYGAYEKQTGRLNRRIRYETVSSAINYLSFALAGLPYMGVGRNLSYKKHLFFN
                     SNGFFDHRHLPSGDDDLFINMVARKKNTAIIINNDAFTYSEPKETWDDWREQKTRHLS
                     TARYYRFKHKFWLGLQPITHILFWLSGIASLSLLYPWWYIVAGTIILRWLLLRYTLGK
                     SMQKLDEKDLKAYIEWYDILQIFYYFRFAKAALVKTKYRWD"
     gene            16933..>17912
                     /gene="tgt"
                     /locus_tag="PLL28_06525"
     CDS             16933..>17912
                     /gene="tgt"
                     /locus_tag="PLL28_06525"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNF69013.1"
                     /translation="MHKLSTITYFCPVFFKLTAKDLHSSARAGELQTDHGSIPTPIFM
                     PVGTIGTVKGVHFQELEDDIQAAIILGNTYHLYLRPGIDTIEKAGGLHAFNSWKKPIL
                     TDSGGFQVHSLAQIRKIKEEGVTFQSHIDGSKHFFSPENVVDIQRSIGADIIMALDEC
                     TPYPCERSYAQKSMGLTHRWLDRGIAHFEATAPKYQHKQFFFPIVQGSVYPDLRKISA
                     EYIASKGAEGNAIGGLSVGEPHEDMYAMTDLVCNILPQDKPRYLMGVGTPANILECIA
                     LGIDMFDCVMPTRNGRNGMLFTADGIINIRNKKWEQDFSPIDINSTSSVS"
BASE COUNT         5163 a         3844 c         3672 g         5233 t
ORIGIN      
        1 ctattcagct ggcatgcaag gctgaggata ccaccgacat gaaaaattca tttattcatt
       61 tgcatgatgc acttagcact ttcgaaaatc attatggtcc ttttcgttgg gatcgcgttg
      121 ggtatgcggt tgtgccattt agcggaggcg cgatggaaca tgctatgaat attgcttatc
      181 ccctctttgc agtcaacggc actacgctct gggaaaattt atatgcacat gaattaagtc
      241 accattggtg gggcgatctt accacctgtt caactccgca agaaatgtgg atgaacgaag
      301 gatgggccgt attttcagaa catttatttc aggaatcatt gtatggtgag caagcctata
      361 tggatgccgt aaaagccaat caactcgatg tattacataa tgccgctccg cgcgatggca
      421 ataattattt cgcgatatcc ggtgttcctg aatcatatac ctatggcgca accacctaca
      481 acaaaggcgc cagcgttatt catacactta gaggctatat gggtgatgac gcatttttca
      541 gttgcattac aagctttatg gaaacatatc aattccagcc tgtaagtgct gccaacctgc
      601 gcgatcattt aacagcatgc agtggcatgg atatgaccga tttttttgat gactgggtgt
      661 atcaacccgg ctcacttgcc tttgatgtgt tgagtacagg tggcaataaa ggaacgatta
      721 cttgtttaca tattcagcag tatttgtctc atgcaacaca ttatggtaat aatgttccgc
      781 tcactgccta ttttttcgat tataatttta atctgttcga ctctgttgat atagtaatgt
      841 cgggcgacag acaattaact accattgaag taccaaatcc aggctcaatc gtatatacaa
      901 tacttgatta caggaataaa ctaaatgatg cggtaacatc cgatgaagtg cattttaccg
      961 aacccggcac gtattcgctt gataatgcca tgatggatat tgaaattaca tccgtaacat
     1021 atccgggttt catttatgcg caacattttt gggctgcacc gaataattat attgccgcac
     1081 atccgggatt acatgtaagc actgatcgct tttggagaat aacaactaat attcccgtta
     1141 ccggtgatgc cacggctaaa tttctataca acggacaagc taccataggc agcggttatt
     1201 tagataatga tttcattaca aattctgaag atagccttgt actactatac agaaacggcc
     1261 cgcaaggtga ctggataatc tatccagatt acacattaaa tacttgggga agtgccaatg
     1321 acaagcgcgg tgctttcgaa ttatctgccg taacatcagg agatttttgc attggaatat
     1381 atgatgcatc cataccggat caaccattat acattccgga aactgattgt gcatttaatg
     1441 gggcaattga gaatttaagc gataactcgc ttacagtata tcctataccc gccaatcaac
     1501 taattgttgc aactgttgat cagcaattga tgggtatgca gttatcgctt tacgacactt
     1561 cgatgcgtaa aatatggaca ggaaggatag ataatctttc catgagcatc cccacacaaa
     1621 acctccccgc cggaacttat caactttgtg ttgaaaacgc ccgttctgag cggattgcgg
     1681 ccgaaaaggt gttgattatg cactaatatt gcgcaaattc ttttgcgcta tgaaaaatgt
     1741 aactgtgatt ggtgccggca caatgggaaa cggcatcgca cacgtatttg ccatgaaagg
     1801 ccacaaggtc acacttgttg atgtttctga acctgccttg caaaaagcgg ttgctaccat
     1861 caccaataac ctcgatcgca tggtggccaa ggaaaaaatc actgcagatg aaaaagcagc
     1921 aacactttcc aatattacca cttatgcaaa ttttgatcac agcactaatg ccgacctggt
     1981 tgttgaggct gctacagaac gcgttgatct taaattaaaa atatttactg aactggatca
     2041 aatatgtgca gcacatacca tacttgccag caatacctca tcaatttcca tcactaaaat
     2101 tgcagcagca acaaaacgtg ctgatcgagt tatcggcatg cactttatga atccggttcc
     2161 ggtgatgaaa ctggtggagg taatccgcgg ctatgcaacc tccgatgaaa cagccaaaac
     2221 tataatggaa ttatctgcag cattggataa agtgcctgta gaagtaaatg attatcccgg
     2281 atttgttgcc aaccgcatat taatgccaat gataaacgaa gccatttata ctttatatga
     2341 aggcgtggcg ggcgtatatg aaatcgatac cgttatgaaa ctcggcatgg ctcatcctat
     2401 gggaccgctg caactggctg attttatcgg acttgacgtt tgccttagca tcctcaatgt
     2461 gttacacgac ggttttggca atccgaaata tgcaccatgt ccgctactgg taaatatggt
     2521 tactgcaggt taccttggtg ctaaatcagg tgaaggattt tatgcttata cggcaggaaa
     2581 taaagacctc gttgtgtcac cacgatttaa taagtaattt aaaaaaaaca atttaagcat
     2641 ctacacaaat cttgatgccc attcattaat agctatcaat aatacataca tgacaccggt
     2701 ttttgccaaa gcatattccc cttcagaagt acatttccag attgaagatt gcgacaatcg
     2761 caatgaacca cgcaaggtaa tgatgtgcac gcccgactac tataatgttg ttgatgtgaa
     2821 aaatgtatac atgcaacatc aaaaaggcaa tatcaacaag gagcgtgcaa atattcaatg
     2881 ggataatatc cgcaattctt atctcgcatt gaaaaaccag aaattccttg atgatgttgt
     2941 tgtcatcaat ggtgtggaag gcatggagga tatggttttt tgtgcaaacc agagttttcc
     3001 ttgggtgatg tggaatggcg agaaaattgc catcatgagc cgcatgcgcc atgaaaaccg
     3061 acaaaatgaa attccatata tcgaagattt ttatcaggac cacggatata aaattgtgga
     3121 gctgcaaagc aaatatcatt ttgaaggcat gggcgatttg attgctcatc ccggaaaacg
     3181 tgttttatat ggagcatatg ggtttcgcag tgaaaaagca gtttacgatg aaatttcccg
     3241 cattgttgat gcgcctatta tcactctcga acttatcaat gagcatcact accatttaga
     3301 tacttgcttt ctcccattaa atcacaccga agtgctaatt gcccctgatg cattcacacc
     3361 ggaatcactg gccattattc gccgcatgtt tgaaggtgtg tatgaaatac cggaagatga
     3421 agccatcgac ggatttgctt gcaatgcaca tattatttat ggcaccaata aaaattcatc
     3481 ggcagctgta attcaacgag gcaatcccgt ttctttgaac atcatccaac aaagtgcaaa
     3541 ccccgtaatc gaaaccgata ccaccgaatt tatgaaaagc ggaggatcag tgttctgttt
     3601 gaaaatgatg ttctattgaa ggagagagga gaaaggagag aggagaaagg agagaggaga
     3661 aaggagagag gaaacgggag agaggatttt ttgaggcttg gaagaatgta ctttgaagtt
     3721 gagtggatcg gttgcgattt tgctcatgga gcgcagcgac attccgccaa agctggactt
     3781 gaacaccata gttaaatatc catgataatt ggaagttgca cattaattta gcaattaaaa
     3841 tcatcctaat attttcaata ccggatacca catgctaatc accccaacca aatgaaaaaa
     3901 atatttataa caggaatagg gaccgatata gggaagaccg ttgtttcggc agtggtagtg
     3961 gaggcattat ttgccgacta ttggaaaccg gtgcaaagtg gtggtttgga ttatacggat
     4021 acacaacgcg tgcagaatct tatttccaat ccaaaaagtc aatttcataa agaggcattc
     4081 ttattgaagc aacccatgtc gccacatgct gccgctgctt tagatggtgt ggaaatagat
     4141 ttagataata ttgtcgttcc tgaaacatca aacacattag tgatagaagg cgccggcgga
     4201 ttaatggtgc cgatcaacaa ctccaccttg atgttggata tgatcgcaaa atgggaagcg
     4261 tctgtgattg tcgtttcaaa gaactatctc ggaagcatca accacacact gttaacctgc
     4321 gctgtgttgc gagcaaaaca aataccggtt gcaggaatta ttttcaatgg tatggaaaat
     4381 gtttccagcg aacaattcat cactcaatat tccgacatcc cggttattgg ccgcgtagca
     4441 catactgacg ttctggacaa accatttata aaaaagcatg ccgaattcat tcgcaagcgc
     4501 atgcagcaat taaaaatatc ctgatatgcg tgatgatggt cctatatggt atccattcac
     4561 ccaaatgcaa ttagcccccg aacctgtaca aatcgtttca gggaaaggtg cactgttatt
     4621 tactgctgat ggcaatacta ttattgatgc cgtttccagt tggtggacga atattcacgg
     4681 tcatgcacat ccctatatcc gacaaaaagt tcatgagcag ttcggacaac tggaacacgt
     4741 aatttttgca gggtttacac acgctccggc aatgcatctt gcttctcgtg taacagcaat
     4801 gtgcggcagc gattatacta aaatgttttt ctccgataac ggaagtaccg ccgttgaagt
     4861 ggcacttaaa atggctttgc aatatcacca aaatcttgga gcaaaaaaac atcgcattat
     4921 tgcattggag ggcagctacc atggcgatac atttggcagt atgagtgtca gtgcgcgtgg
     4981 tgcttttact gccgcttttc acgataaatt atttgacgtc acctatatca cccctcccct
     5041 tccgggaaat gaagacattt gtatgcaaga attagaagca gcgttgtcgg aaggagatgt
     5101 ggccgcattt atttacgaac cacttatcat gggagccgga ggaatgctca tgtataccgg
     5161 tatggcttta gatacaatga tatacaccgc caaacaacat ggagtactat gtattgccga
     5221 tgaagtaatg acaggatttt accgaaccgg aaaattcctt gcgggacatt atgccaatca
     5281 tcagccggat attatatgct taagtaaagg tatcaccggt ggatcaatgg cactcggcgt
     5341 tactatatgc acgcaagcaa ttttcatggc attctatagt gaagataaaa tgaaggccct
     5401 ttatcacggg catagctata cggcaaatcc tttggcttgt gctgccgcaa atgcaagtgt
     5461 cgatttatgt ttgcagcccg gttttgatca tgcactcaca accatagctt cacgtcatca
     5521 acattttaca gccgcattac aagtgtacag tcacagtgat atacctgtgg taaaaaatat
     5581 cagacaatgc ggaacagtgt tagcaatgga ttttgttact tccaacgata ccggatattt
     5641 taacagcata cgcgacatgc tctatcattt tttcatgaaa cgaggtgtgc ttctgcgacc
     5701 attaggaaac accatctata tcatgccgcc atattgtatc accaacgaag cattggatga
     5761 agtgtataat gccattttag atgcattggc attgttagcc gaaggaaaga cattacaata
     5821 attatttcca ttcaggtttt ttcttctcca ggaaggagcg aataccatat tgacaatcat
     5881 cagttgccct ggcctttgca ttcatttctg ccgcatattg caatgcttca tccaatccca
     5941 tttcctgcac cgccgctatc atcgattttg tagtggccac tgctacccta ctcgtctgag
     6001 ctatcaactc aacacataaa ccggatacag cttcgtcaat tttatttttg ggaatcactg
     6061 cattgatgag accatatttt tcagcagttt cagcagttat taatttgccg gtgagcaata
     6121 attcttttgc atgtcgctca ttaattttac gcagcagaaa taccatcaca atagcaggaa
     6181 taaatccaat cttcacttct gtatatccaa actgtgcttc cggaacagca aaactgaaat
     6241 cacatacact cgccaatccg caaccacctg caatggcatg accttcaatt tttgcgatga
     6301 tgggtttcga taaggtatac atcaatttat acaaggccat taatttttga gaatcggcta
     6361 cgttttcttc aaaagaattc tgttgcagcg attgcagata tcccagatcc gcacctgcac
     6421 taaatacctc ccctgaggca gcaagcacaa tcacttttac agattcattt tcctgagcat
     6481 cggtaaatgc ctcagttaat tcagctacca catccgcatt gaatgcattt cgcttatccg
     6541 gacgattaag ggtgatggtg cctactttat cggctatagt ataaaggatg gttgagtatg
     6601 tcatgcctca aaaatcaatt ttgtaataga acaaaggtaa aaagcctaac tgatactctt
     6661 ctttcaccgg actggccgtt ggatctgcag ggtttggcgc ataagttaat cccaacacgt
     6721 tttgaatatt caatacattc acaagatcca gagcgatttc gtgcgtgaca ctacgcttac
     6781 cggaatttat tcgataagca agcttcacat caaaacgaaa ataatctttg agttgtatcg
     6841 tatttgtcag agaatcaata tagatcagtt cctttgctgc aatagtagct gctgtgtcga
     6901 cgggcgtagt gagtttacct cccgcatagg ttgttttaat gccaacggta agcacattgc
     6961 ggtcgctgat ggtgaattca taaccgccga gcatattacc ggcataggtg ccattaaaat
     7021 cggttttgcg ttcaatgcca tctgacccaa catatcggga atcgtataat gatccggtta
     7081 tcaggaaaaa gaaatgattg ctgaaaaatt tctctatggt gagttcggca ccatagtttt
     7141 tacctgttcc tgtattctgt aatgagtcag ggaaaaatct tgagaaccca ctcccttcgt
     7201 ttgccaatga aaatgcgctt ggtgcaactt caaccggcac atcatagaga tattgataat
     7261 atacttccga tttaactcgc agattatcgg agagctttct atcataaccg agtatcatat
     7321 gtgcactacg gataaatccg acatcttcat tgatgagcaa tgcgcttcca tctgcctgat
     7381 aatgcatata ggtatactga tagcttggca acatctggct gtgcaatccc aaacccaggc
     7441 ttaacgaaga cctgtcatcc acacgccatt gcataccggc tctgggttcc agggattttg
     7501 aattgctgtt gatggtaaat atctgggcat gcaagccacc ggtaaacaac aggcgttcat
     7561 tgtatttata tttccattga aagtaagctt gtgccaaata attattccct tcaaaatcat
     7621 agcggttatc ccaagtccag gtatacacat ttctattaga atcgatatga ttataccata
     7681 ttgattcaaa gcttgctcct gttttaaaac tatggcgact gttgactttt ttattcaatg
     7741 aatatatcag cgatgctttt tgatcgagga aagaataatt catattcggc actatggaat
     7801 ctatatcgaa attcacatct ctgaatacga aagcatgatt agcatgattt tcctgtacac
     7861 tatatgcagc aacccattta ccatatgctg atggattgta ttgcctggca tatgaaacac
     7921 ctgcaaccga catagctgta ccaaagtatt gatctctgtc gctatctccg tataattcag
     7981 tttcctcctt agtgtattca cttaccaaaa tatctatctt actttttccg ccgatggcaa
     8041 atacattcca catggcatta ccttttggaa aagaaagttt aaatgctccg tccatataat
     8101 ttggcacagc ggtagttcct atcggaatgt gcatgaattg aaataattgt aaagttgaat
     8161 atcgatagct cgccaaataa gatgaacctt tcgatttatt gataggccct tcggcaaaca
     8221 attctgttcc aagaaatcca agctgtccac tgaactcatg ttgttcatca ttgcctcttc
     8281 gcatattcaa atcgaaaaca ccggcgatag cattgccata ttctgcagga aaagcacctg
     8341 taaaaaagtc ggagttactg aggtatttat tattgataat tgataccgga ccgccggtag
     8401 taccggcaat tgcaaaatga cttggatttg gaatgtcgat accctccaat cgccacaaca
     8461 cgccgattgg tgaatttccg cgcacaacaa tatcattacg tgaatcatca gcaccttgca
     8521 caccggcaaa atttgatgcc atgcgagcag ggtctccgcg actgcctgca tagcgatctg
     8581 tttcttcaac actgaaggtg cgcacgctat tgattgccat ttcattattt acctcaccct
     8641 tgtccgattt ggccgtaaca acaactgcat caagtgcttc ggcaccttct tccaattcaa
     8701 cattgataat cacttccttc ccgccggtaa ctattacatt gctcatggtg atgtcctcat
     8761 aaccaacata tgtaaatcgc agggtaacac gccctaccgg agcattggta atgcgataat
     8821 caccttgttc atcggtggta gcaccaccaa gcatggttga gtctgcatac aaaaggacag
     8881 tagcgccgat aagcggatac ttcgatgctg cgtcggtaat agtgcctcgg acggtttgac
     8941 ccggcgattg tgcggttaga cttatcgttg ataaaagcaa gcaaataagc agaaaattgc
     9001 gcatgaatat gggtttaaca ctcaaatata accctatgcc tttgagccaa caggcaaaat
     9061 atgcacattc cgacaaagtg attattgatt ttcgcaaatg cttacaatga ataccctagg
     9121 gcctctgaag ctactaacaa caggttttgg aacattcttg gcacaaaaaa ggccgccaaa
     9181 aaaggcagcc tttcaataaa atcaattaaa aattactttg ccggcaccat ggcagtgatg
     9241 gcatcacaac tttgcttaca agcatcttgc attccgctga gttgttgatt caagccttca
     9301 actgtagttt tccactcagt tagttttgtc gtccagtttg cgagttctga ggtaatagtt
     9361 gcatcatcaa ggtgctcttc ctgtcccttt ttcttccatg caccatactc gtcagttgct
     9421 gcagcgaaat cggtttttgc ttttgcaaat ccggttttca tagcgaccat ttgctcatca
     9481 attttgctgc acatcatcag gttcatttct gccatggatt ttgattcacc ggacaattta
     9541 gtaatgtcca tgcttctggt ggcagtcatc atttgtgtca tgccatccat tgctgtgttc
     9601 atagtagttt cccaagcagc gatttgatcg ccgagggctt tccattcaga ttcgaatgtt
     9661 gccattgcct gttttgtttc tgcgcttggg cctttctttt tgccgcaaga atttaaagtt
     9721 gccattgagg cgattgccag aatgacgagg aatccttttt tcattttgtt atggttgagg
     9781 tttaaattaa agatattgtt attggtaaaa tcaatttccg aggttacgta agctttcttc
     9841 caatatgcgg attttttctt cagcatcagc ttttttgcga cgctccaaat ctacaacagc
     9901 agcaggtgcg ctgccaacaa atttctcatt gctgagtttt ttgtcaatgg aaaggaggaa
     9961 tcccttttga taggcaattt gttcttccag ttttttgcgc tcatcttcga tattaatttc
    10021 catctgcaca acaacaaaaa acttttgcgt tcccacaagg aaagtgactg cattttttac
    10081 atcgccggtc acagattcaa acacttcgag gtttgccatt ttcatgatga cagcgcggaa
    10141 ggcttgcatg ctggtgatat cgccttcagc atacaaactt gcagcaacct ttggactcac
    10201 attattctta gcgcggatat ctcgtatcct gctgattaat tccttcgctt cgttgcctga
    10261 tgcaagaata ttgttattga tattacccgc gtcattcaat tgtgtgatta caatatcatc
    10321 tccggctttt ctatcacgca gcaaatgcca aatttcttca gtgataaagg gcatgtaagg
    10381 atgcaacaac ttcaggcaac cttcaaagaa atctattgtt ttttcatagg taatactatc
    10441 aataggctga ccaaattccg gtttaataaa ttcgaggtac catgaacaga aatcgtccca
    10501 aatgaaattg tataaacaca tcaaggcatc gctgatccga aactgttcaa aatgatcgtt
    10561 gatggtgttt atagtatcat ttagctttga atcaaaccat tgaatagcgg cttcattttt
    10621 cgtttgcgac gtttcggtca cttcccatcc tttgatgagt cgcagtacgt tccacaattt
    10681 attattgaaa tttcgacctt gctctattaa tttatcatcg tagatgatat catttccggc
    10741 aggagagcac aataaaattc ccatgcggat agcgtctgca ccatattgag caatgaggtc
    10801 gagcggatca ggcgaattgc caaggctctt gctcatttta cggcgcaatt tatcacgaac
    10861 aatacctgtc agatatacat cgctgaaagg tttctcgttg cgatattcat aaccggcaat
    10921 gatcatacgc gccacccaaa agaataaaat ctccggtgca gtaacaagca cttgtgtcgg
    10981 ataataatat tcaatatcag cactcttcgg atccttcatg ccattgaata ccgaaatcgg
    11041 ccacaaccag cttgatgccc aggtatccaa tacatcttca tcctgacgga aaccatcgag
    11101 cgacataccc ttcgaagtaa atatagccct tgcttctgct tctgttttcg caactacaaa
    11161 ttcaccactt gcattatacc atgcaggaat gcgctgtccc caccacaatt gacgactgat
    11221 acaccaatct ttcacatttt ccatccagtg tcgataggtg ttgatgaatt tctcgggatg
    11281 taaattaata tcgccattca tcacatgttc aagggcaggt ttgctgatct ctttcatgct
    11341 gatgaaccac tgcatactta atctaggttc gatcatggca tttgtgcgct ctgagaaacc
    11401 tacactattt ttcagctctt caattttcac aagatagcct tcctcttcaa gcatcgctac
    11461 cattttcttg cgcgctacca tgcgatcttc accgatcagt atttctgctt tttcactgag
    11521 cgtgccatct tcattcatga tgtcgataac cggcagatta taacgcaagc ctatttcata
    11581 atcgttagga tcgtgagcag gtgtaatttt tagtgcaccc gttccaaatt ccatatcaac
    11641 ataggtatct gcgataaccg gaatttctct tttgatgagc ggcactaata cagttttgcc
    11701 aataaattct ttatatcgtt catcatcagg atgcacacaa atggcagtat ctcctaaaat
    11761 ggtttcaggg cgtgttgttg caaccgtcag ataaacatta tcgcccttga tttgatattt
    11821 cacataatgc aaacgcgaag taacatcctg atagattacc tcttcattac tcaaagcggt
    11881 gcgtgcttcc ggatcccagt taaccattct gaggccgcga tagatatatc ctttattata
    11941 aagatcgatg aatgtatcaa tcacatcgtc ataataaaga tcatccattg tgaatcgggt
    12001 acgatcccaa tcgcagctgc aacccagttc ttttaattgt tgaaggatga tgcctccata
    12061 tttctctttc cattcccatg catgcttcaa aaaatcttca cgagataatg acgacttttt
    12121 aattccctgt tcacgcagca tcttaaccac ctttgcctca gtagcaatgg aagcatgatc
    12181 ggttcctggc acccagcagg cattttttcc ttgcatacga gcacggcgga tgaggacatc
    12241 ctgaatcgtg ttattcagca cgtgacccat atgcagcacg cctgttacat tcggcggtgg
    12301 aatgacgata gtaaaaggct cacggttatc gggttttgaa tgaaaatacc tgtttgctaa
    12361 ccagtgtgca taccatttct tctcgatcga cgcgggatca tacgttttgg aaatctccat
    12421 agcgctgcaa aattacgaaa atggaaggag gcatttcgcc ccgatttcgc atctttgtcc
    12481 acgtgaagaa ggcatatttg ttggtattgg tggctattgc ctccgttttt caggcagaag
    12541 cgcaaatgca ggcacatgat acctgtaggt ttatgacgta taatgtgttg aatttcgatt
    12601 atacaggcaa tttcaaggag aattatctgc gcactattgt atccgaaatc catcctgata
    12661 ttatggttgt gcaggaactg atagacgaca gcggggctgc acgattcaga gattcggtca
    12721 tgttgcaggt agataccaac tacgccatgg cggaattcat caatagttat gataccgata
    12781 atggtttgta ttaccgcaag gataaatatg cctttttatc gaataccgtt gtgactacag
    12841 aattgcgcga tatcaatcaa ttcgaaatgc ggcatattgc aagcggcatc gacttttatg
    12901 tttactctct tcatctgaag gcgagcagcg gcggcacaaa tgcggcacaa cggcagcgcg
    12961 aggtagatag ccttcgcaag gcaactttgg ctcttccaga cacggcacat atcttggttt
    13021 gcggcgattt taatatttat ggcagttatg aggaagctta cctgcacctg ctggatccta
    13081 ccgatccggg ctatgtgata gatatctggc aggatagttt gacgagcacc tggaataata
    13141 ttgccaacac cgattacctt acacaaagca caagaattgc tgcttttggg ggtggatcga
    13201 ccggtggtat ggacgatcgt tttgacatga tattgatgtc gccgtcgtta gtagatacag
    13261 ccggaatggg ctatatcccg ggaagtatgt atcctgttgg caacgacgga aacaggttcg
    13321 ataatgagat caacgatcct gtgaatacct cagtttctga agaaatggct gatgctttgt
    13381 atttcacgag tgaccatatt ccaatgtttg ccgaattctc gttcagctat atcattcctg
    13441 aggaacctga ggatactacg gatacactga ttcaacaaat agcagaaaca gacttgttgt
    13501 tgcaactcta cccgaatccg gcgggtgatg taatcactgc aacgtcaaat tttaacgctg
    13561 aaggatatat catctatggt gcagacggca aaactgtata taaaatgacc gaaaagtcaa
    13621 taccggcagg catttcagag aaaattgcca tagcagacct ggcacccgga ttatatgttt
    13681 ttgtgtcgtt taatggaatt aaacgggtgc aatctacctt cctgaaaaat taaaatcatc
    13741 ggctttggcg atttggtaag ctatctttgc ggcttatgca acagttcaaa gtaggcatta
    13801 tccgtgaggc taaatttccc cctgatgcgc gtgtaccgct tatccctgct caggtacaac
    13861 aacttcgcca acagtttccc aatattgaaa ttgtagttca gcccagcaaa ggccggtgct
    13921 atgctgatta tgaatacaca gatctggatg tgacattaca ggaagatttg cacgactgtg
    13981 atattctctt tggtgtgaag gaggtgcctt atgacctttt aattccggga aaaacatatt
    14041 ttttcttcag tcacaccatc aaaaaacagc ctcacaataa aaagttatta caagtgctga
    14101 tgcaaaaaaa cgtgcgcatg atcgattacg agtgccttaa aaatactgaa ggaaatcgtg
    14161 tgatcgcttt tggcaggtgg gccggaatta tcggtgcaca taatgccatt tggacctatg
    14221 cgcaacgatt aggtgaacaa cacttaaaac gagccagaga ttgcagggat tattatgaat
    14281 taaaatatca ataccgcgga ctgacgatgc ctgttattaa aatgatcgtt acaggtacag
    14341 gaagagttgg caaaggcgcg attgaatttc tgagaaagat tggtgtcaga gaggttacca
    14401 aagaggctct gctcaacgaa acattttccg aagcagtgtt ttgtgtactc gacagcgatg
    14461 atttgtattc ccgcaagtcc gatggcaaat atgaccgcaa tgaatttcac agccatccgg
    14521 aattatatca ttgcactttt tccccataca caaaggtttg tgacgtattg atcaattgca
    14581 ttttctggaa tcctgcggca ccttcattat ttcacaaaaa tgaaatggat gccactgatt
    14641 ggaaaataaa ggtgattgct gatgtcagct gcgatgtgaa aggcggtatt cctgctacta
    14701 tcaggtctac aaccataaat gagcctgtat ttggctatga tgtagctaca gacatggaga
    14761 ttgcccccta cattccggaa agcatcgata ttatggcaat tgataattta ccaaatgaat
    14821 taccgcgaag tgcaagtgaa gaatttggac agcaattaat ttcaacggtt tggaatgaat
    14881 tatttcaaga taactcccgt atgcttgatg aagctaccat ctgcaaaaat ggcaatctga
    14941 ataagcattt cgaatattta cgagattatg ttaccggtga atcgtgaatc gtgagtcgtg
    15001 agtcgtgaat cgtgaatcgt gaaattggtg cattgggaat tgtatttcac ccgcctgctc
    15061 ggcgaggtaa actcacccac tcacccactc accactcacc actcaccctt ctcctctctc
    15121 ctctctcctc tctcctcccc atcacatttt agcccttcta tccgccaaaa tttctgcgag
    15181 caggtttctt gcacggaaga gctgagcttt tacggtcccg agtggtatgt ttagtgtttc
    15241 tgaaatttcc tcataagata gttcgtcgaa atagcgcatt tcaaccagtg ctttatattt
    15301 tggatttaat tcatcgagga tggtgcgcat gatattagcg cgttgttcct taataaactt
    15361 ctcttccgga tcgggattgt tggttctgat attagtaaat gccgatcgtg tgaattcatc
    15421 ttgttcatca tcgaggctga gtgtatgcaa tcgttttttg cgaatgaaat cgatagcatt
    15481 atttacggcg attttaaaca accaggtgct gaaagcgaaa tcaggcgtat agcgctcgag
    15541 gtgcaagaag gctttgccaa aagcttcaat ggtaaggtcc tcggcatcgt cgcgattgtg
    15601 caccattttg agtaccatat aatgaatggt atccttgtag cgcgacatta attcaccgta
    15661 cgcgcgttga tcacctctgc gtgcactttc tacaagtgcg agatcttcct ttgcgcggtc
    15721 tgataaattg tcgtttaatc ccatctatat tttgttttca caagggcagc tttagcaaag
    15781 cggaaataat agaatatctg caagatatca taccattcga tataggcttt caggtctttt
    15841 tcatccagct tttgcatcga cttacccaaa gtataacgca ataacaacca gcggagtatg
    15901 attgttcccg caactatata ccaccatggg tataagagtg acaatgaagc aataccggaa
    15961 agccaaaata agatatgtgt tataggttgc aatccgagcc aaaatttatg cttaaaccta
    16021 taataccgtg ctgttgaaag gtgccttgtt ttttgttctc gccagtcatc ccaagtctcc
    16081 ttaggttctg aataggtaaa agcatcatta ttaataatga tagcagtatt tttcttacgt
    16141 gccaccatat ttatgaacaa gtcatcatcg ccactaggca aatgacgatg atcgaaaaat
    16201 ccattcgaat taaagaagag gtgtttttta taagatagat ttcttccaac acccatgtac
    16261 ggtaaacctg ctaatgcgaa gctcaggtaa ttgattgctg aagaaaccgt ttcatagcga
    16321 atacgtcggt taagcctgcc ggtttgtttt tcatatgcgc cataacccag cacgatctct
    16381 gctcccgaac cataaccatt catcatttcg cgcagccatt tatctgatgc aggatagcaa
    16441 tcggcatctg tcaacaaaac tatttcatgc gacgcagctt tcaatcccaa tgtgagcggg
    16501 aatttttttc ctgccatcat tctattactg ttgcgtgtac tggtgcgctt cagtcttggt
    16561 tctttttgag aaagaaaaaa taagtattct gtagtactat cctctgaatt atcatccaca
    16621 acaattacct caaattcagg ataatcctgt gataaaatgc gtggaagatt tttctttaga
    16681 ttagaatctt cattccttgc gcaaataatg atgcttacgg gtggcaatgc atgatcctgc
    16741 atgacagatt tctgctgata tcttgccaaa cgcaaataaa aaaacaatat gtagaataac
    16801 tgcaccgccg cagcaccaca aaacaagtaa aatatactgt cgaggataat tgagtcgccc
    16861 ataattgtgc tgccgcaaag ttagagtttc aaggcattgc aaggcgtttg tgatgttatt
    16921 agcacttcac atatgcacaa gctttccaca attacctatt tttgccctgt gtttttcaaa
    16981 ttgacagcca aagacctgca ttcatctgcc cgcgcaggtg agttacaaac cgatcatgga
    17041 agcatcccca cacctatctt catgccggtt ggtacgattg gcacggtaaa aggtgtgcat
    17101 ttccaggaat tggaggatga tattcaggct gccatcattc tgggtaatac ttatcattta
    17161 tatctaaggc cgggaatcga cacaattgaa aaagccggag gattgcatgc atttaattcc
    17221 tggaagaaac caatattgac cgatagcggt ggctttcagg tgcattcatt ggcacagatc
    17281 agaaagatta aagaagaagg cgtaacattt caatcgcata ttgatggctc taaacatttt
    17341 ttctcgccgg aaaatgttgt agatatacaa cgtagtattg gtgctgacat tattatggct
    17401 ctggatgagt gcactcccta cccctgcgaa agatcttatg cacaaaaaag tatgggattg
    17461 acacatcgtt ggcttgacag gggtatagcg cactttgaag ctacagcacc aaaatatcaa
    17521 cataagcaat ttttctttcc tatagttcag ggcagcgtat atccggattt aagaaaaata
    17581 tctgcagaat atattgcttc aaaaggtgcc gaaggaaatg ccatcggcgg attaagtgtt
    17641 ggcgaaccgc atgaagatat gtatgccatg acggatttag tttgcaatat acttccgcaa
    17701 gataaaccgc gttatttaat gggtgtgggc acgcctgcaa atattcttga atgcattgct
    17761 ttgggaattg atatgtttga ttgtgtgatg ccaacgcgca atggcaggaa tggtatgtta
    17821 ttcaccgctg atggcatcat caatattcgg aataaaaagt gggaacagga ttttagtcca
    17881 attgacatca atagcacgtc ttctgtgagt ag
//
LOCUS       DAOQHY010000059        17625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1490_length_17625_cov_7.141605, whole genome shotgun sequence.
ACCESSION   DAOQHY010000059 DAOQHY010000000
VERSION     DAOQHY010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 17625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17625
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1490_length_17625_cov_7.141605"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..1254
                     /locus_tag="PLL28_06530"
     CDS             118..1254
                     /locus_tag="PLL28_06530"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNF69014.1"
                     /translation="MKRQTFIILTILFSLTACSQKDKQTISTPQTLDDGWKISEPDNA
                     GFDKQKLEKLIRGISSENPKLNSLVIARHGKLIADEYFNGYTPDSLQKIWSVTKSITG
                     TIIGIAVDKGLLSEKDSIYKYLTDYIPNANSSEGAITIEHLMTMTSGFEWVELGGPKS
                     AGFQLAYSTDWIAFTLNQPHTNVPGTIFNYSTGNAMLLAPIVKKATGQQANEFAKDNL
                     FLPLGITNYEWDTQSEFWTKTQSGELPGAKQPTDIDYKKPFSDFTNTGSGLRMRSRDL
                     CKIGQLYLNRGKWNENQIVSEKWINKSTQSHFGNSDYGYLWRLMNFQNNSCYYATGFG
                     LQRIFVIPTLDLVVVMTQQHYETMPQGEKWINQFLTELLETIKE"
     gene            1251..2471
                     /locus_tag="PLL28_06535"
     CDS             1251..2471
                     /locus_tag="PLL28_06535"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNF69015.1"
                     /translation="MKKLQQAAKIFKGQMIKRQSTISTLFLLLTIIYSSCNGQVNTYP
                     TKDKQLKIITNNFLPELAEEYKYVQCGLQDTKGNMWFGTAGNGIYVYDGDSFVNFTHK
                     SFINFSLKEDLNHNDILCCKEDIHGNIWFGTRRGLIRYTPSKEKPEAKDFTLILIPAN
                     TINNSSPNRLPYTLQSGDNFVWSIMQDKSGKIWFGTSRGIYVHNPSTDNDSIGPLFKR
                     FLDQDSLTNNQHLQLLNVTSMQEDRNGNIWFVSPNWTANKGEGIVRYDGKSLVTFTPD
                     STYSFRSIIGRNNGDLLFLSPFNGVYSYDGKTFSNLTENIGIKNDTLMSIMEDKNGNL
                     WLGHISENVKNGGEGGLWYYDGKSLKLFTINDGLPHNHVMCIVSDKEGNIWFGTRNTG
                     LCRYDGKTITNYSD"
     gene            2614..3000
                     /locus_tag="PLL28_06540"
     CDS             2614..3000
                     /locus_tag="PLL28_06540"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNF69016.1"
                     /translation="MKKVKIKSHIELYELLPENERIIVDVLRQIVKATLPSNCKEKIS
                     FSVPYFYGNKGICIIWPATIPRGGIKKGVLFGLWYGNRLKDPDNYLTHGTNKQIFYKI
                     YNSPEEIDFKKVVKLLKEAVVLDTQW"
     gene            3330..3548
                     /locus_tag="PLL28_06545"
     CDS             3330..3548
                     /locus_tag="PLL28_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69017.1"
                     /translation="MENIIRHFDKYLPLGDNEKEALTSRLIERKLKRKQYLLQEGEIC
                     RYFSYVVEGCINLQITLTTSFLDVTLRV"
     gene            3597..4604
                     /locus_tag="PLL28_06550"
     CDS             3597..4604
                     /locus_tag="PLL28_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69018.1"
                     /translation="MKTLSINRLILAIGIPALIIMVLFALTKTELLIEKPTFGAFAIS
                     FDLLITVPCIYYIIIRNSKISPLTLFPMVIATMVIATLILPRENQYYLDLFKRYAIPL
                     IELGFILLVIRGIRRSVRSHQMAGQIKYDFYSVLQLVCYDLLPKRIAGIFITELAVIY
                     YGLIQWKQRPIQSNEFTYHKSSGTPAMMGMILFILAVETFATHLLIAKWNGTAAWILT
                     GLSGYVIIQLLGFIRSLSKRPMFIMNNILFIRYGILAEVSINIDDINSVEFSNRKFKD
                     NHHTRKLSAFGNLESHNVIIKLKNPYTLSRIYGIKKQFTELVLLVDDKQGFKHAIEAA
                     K"
     gene            4768..5253
                     /locus_tag="PLL28_06555"
     CDS             4768..5253
                     /locus_tag="PLL28_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNF69019.1"
                     /translation="MRNLNIIAILMVMSLLFASCGGKNTPAANGEAPKSMLGPKTENE
                     KMIADGRGIGEVKSVTLNTPLEQERVKRGQDIYDMKCSACHKLTDERFVGPGWKGVTS
                     RRKPEWIMNMVTNVDVMLERDAEAQKLLELCLTRMPNQNVSIGDARDILEFMRSNDGE
                     K"
     gene            5292..7268
                     /gene="nosZ"
                     /locus_tag="PLL28_06560"
     CDS             5292..7268
                     /gene="nosZ"
                     /locus_tag="PLL28_06560"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HNF69020.1"
                     /translation="MKNLTLMLLVTMLTIAGCKPKTGTSVASGDAASKVYVAPGTYDE
                     FYNFVSGGFSGQLAVYGIPSGRLFREIPVFSEDPETGWGYTEETKPMLMTSHGYVPWD
                     DFHHPALSTTNGVHDGRWAFGNANNTPRIARIDLKTFRTAEIIEIPNSSGNHSSPFIT
                     ENTEYTIAGTRFSLPPDYEGANTDVSISSYKDNFKGYLSFISVGKETGNMDVAFQIQI
                     PPLNTDLARGGKGPSHDWFFFSCYNSEKAFTLLEVNASQKDKDFILAVNWKKAEEYAK
                     AGKGQHQKVKYTHNTYDEKTHSATSEIKTDVLVLDPKELKDVLYLIPCPKSPHGCDVD
                     PTGQYIVGSGKLAALIPVFSFEKIQKAIANNDFEAEYDGIPVLKYESCLHGEVQKPGL
                     GPLHTEFDDQGNAYTSFFVSSEIVKWNVKSLQVLDRVPTYYSIGHLCVPGGPTAKPWG
                     KYVIAYNKITKDRYLPTGPELTQSAQLYSIEGDKMKLLLDFPTTGEPHYAEAIPASLV
                     QPNSTKIFKIEENNHPYAAKGEKEAKVFREGNIVHVVMTSIRSHFVPDNIEGVKMGDV
                     VYFHVTNLEQDWDVPHGFVVKGANNAEMLIMPGETQTLKWEPDRVGVFPFYCTDFCSA
                     LHQEMQGYIRVSPAGSSVPLTFSTGKTAAEPPAK"
     gene            7306..7866
                     /locus_tag="PLL28_06565"
     CDS             7306..7866
                     /locus_tag="PLL28_06565"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HNF69021.1"
                     /translation="MKKLLYILPLCLALQFGCNNAESTEASGNNDTMNAQAGGQEAVQ
                     DDVSQKDVVKIAVGSPDHTTLVAALKQTEYVNDLSNAGPFTVFAPTNAAFDKLPAGTV
                     DDLMKDENQAALRNILEYHVALGTYRKEYLNNGQTIGMANGDNVTIGINGDDVTVNGN
                     KVLAIVPASNGIIYIVDGVLLPPAKK"
     gene            7960..8556
                     /locus_tag="PLL28_06570"
     CDS             7960..8556
                     /locus_tag="PLL28_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69022.1"
                     /translation="MKKIHNYSAIAVAIAALCFLATFKFPFWVIYLTAPQYPEGLTLY
                     IWLHKVSGDVEIINGLNHYIGMAIIDETQFPEFDILPYAIGFMVGAGLLIAFLRKKKL
                     LFFYTIFLVLFSILAIYDFWHWEYQYGHNLDPDAAIQVPGMSYQPPLIGFKQLLNFGA
                     YSFPHGGGFFYISGVVICCIASILEWRRGKKQLTPKIR"
     gene            8558..8986
                     /locus_tag="PLL28_06575"
     CDS             8558..8986
                     /locus_tag="PLL28_06575"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HNF69023.1"
                     /translation="MKSLSYLSFICIMLLSACSVKPHPINEGEDACAYCKMTVAEKKY
                     ATEIVSNTGKIYVFDDMHCMKSFIKENIVPSGDIKLMMVSDFYNPENLIPADNALLFT
                     SESLHTPMNGKTVGLADSGALKRIQQEMQGNSLTIQQLLQ"
     gene            9003..10238
                     /gene="nosD"
                     /locus_tag="PLL28_06580"
     CDS             9003..10238
                     /gene="nosD"
                     /locus_tag="PLL28_06580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HNF69024.1"
                     /translation="MLRFIWIWILIHCSLGAQSKTIHVNNHKGLAGVQDAINIAQLYD
                     EILLEEGVYTGEGLYINKPIILRGNNNVILDGGEKVTILTIEGTAALVEGLTFRNTGY
                     SSVEERCAIKLLSARGCIITNNKIEHCCFGIMLMKSGHSIITDNNINATNIHEQRSGN
                     GIHGWQSDSLTIRNNNIQGHRDGIYFEFVTCTGVYNNKSINNLRYGIHFMFSNNDEYI
                     GNTFSDNGSGVAVMFSHHVNIRNNAFDNCMGGASYGILLKEINDSQIINNKFRHNTNG
                     IFMEGTNRITLQRNLFLSNGWACRVQASCSSIYLNENNFIGNSFDIATNGSLSINNFN
                     GNYWDKYEGYDLDKNHIGDIPFSPVSMYGVIVERMPYTILLYRSLMVYLLDRSEKVLP
                     GITSEQIKDKQPLMHAVNL"
     gene            10235..10960
                     /locus_tag="PLL28_06585"
     CDS             10235..10960
                     /locus_tag="PLL28_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF69025.1"
                     /translation="MIQIDHLSKSYKSVHALNDLSLTFTAGQSIALTGPNGSGKSTLM
                     KCLLGLVFPDKGNITVNNLRVDKGSEYRRFIGYMPQIVQFPENMRVRQLFKMMRDIRQ
                     HDGNYDEDLYTAFDIQGMADKYLGSLSGGMKQKVNAGLALLFNPKIIIMDEPTAGLDP
                     LSAELLKDKLRKEKEKGKLIIISSHILSDLEEITNHVLFLMDGKVICNKSLPELIAES
                     GTENLSRAIAKLMDPKIAGNETR"
     gene            10947..11711
                     /locus_tag="PLL28_06590"
     CDS             10947..11711
                     /locus_tag="PLL28_06590"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNF69026.1"
                     /translation="MKLVKYVFWDIARNKILIGYAMFLFAVSFGLFSFSDDPIKATSS
                     LLNIVLIVLPLFSVIFATSYYYNAYEFTTLVLGQPINRLHLFFCQFAGVCIAMTLAFI
                     MGCGIPIIMYMPNIIGIMLLLSGISLTLVFISLAFMAGVLARDKARGIGIALLLWFYF
                     AFLYDALVLMLLFAFSDYPMENTMMTLAFLNPVDLIRIIMLMQLDMAAIMGYTGAVFN
                     KLLGSSSGTILCFAAILVWIALPLTISALRFRRKDI"
     gene            11883..12140
                     /locus_tag="PLL28_06595"
     CDS             11883..12140
                     /locus_tag="PLL28_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69027.1"
                     /translation="MTLRGSWDDFGNSNLYGNIISPTEDILIDGLAFQKPSLTLNYTT
                     SSIFPGTTSADDTYIPLDTIAAIRVEDEKVYLRISTLSFLK"
     gene            12137..12502
                     /locus_tag="PLL28_06600"
     CDS             12137..12502
                     /locus_tag="PLL28_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69028.1"
                     /translation="MTITDYAPQYGGLPLDTTFILYDFGLQVNYTVFYPIDHTPVMNE
                     SMIDSVLLNDGIYHQRYHFYETGAIEQVWLEGIGSENGIFGAFLPIAAYMNGSSWLEC
                     FKRGDTYVYGTDSCDFNLH"
     gene            12795..13721
                     /locus_tag="PLL28_06605"
     CDS             12795..13721
                     /locus_tag="PLL28_06605"
                     /inference="COORDINATES: protein motif:HMM:NF039745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69029.1"
                     /translation="MKGKFLIEQYACLAAGFLLLNKQSDSQVVYTDVEPDWIFQDDGD
                     FSYLDMNDDGINDFGFLKSNHIISTLSSPGLNTTWYYRIALWVGPAMVGPDIAGISAH
                     ASSAAGTYCFPYPLEKSNLINNDLSFQNCGFQLLASKTAEVYYGYSYTFLGIGDWHIG
                     IGEFNNLNLDSTRYIGVRFEDEDDCLHYGWIRCAVMDSIGKLVVMDYAYEMQCNKTIA
                     AGNTVSYVDINSETTDSGISIYAFDGKIYFHGNSAGKMVITVSDITGQIVAHQCISGN
                     DKTINMNFEPKGIYVVNVVCNGKYFNKKVVID"
     gene            complement(13886..14896)
                     /locus_tag="PLL28_06610"
     CDS             complement(13886..14896)
                     /locus_tag="PLL28_06610"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNF69030.1"
                     /translation="MRNVRIWLWTGVVMILIQVMLGGITRLTESGLSIVEWDVVMGSM
                     PPTSQSAWEEEFAKYQQTAQFEKVNTDFTVSDFKRIYWWEFIHRLWARTLGFVFLIPF
                     IWFWIKKQLNASWKKKLIFVFVLGGLQGFVGWVMVASGLDGDPWVDPSKLTIHLLLAL
                     TLYMYLIWLAQDTYCKIAHKPIAKGNMNWITALIFIQIGLGGIMAGTDAGRLFNTWPL
                     MNGAFLPDGAFSNLSQAEKLTQLTTEINFIHRTFALIVATAVIIYWYRNRHTGSQKLK
                     QLNLTMVLLVVLQCVLGVLTLLNGSLQIPVFLGVAHQITACILLATAVLIMHRVKHDG
                     AS"
     gene            14987..16114
                     /locus_tag="PLL28_06615"
     CDS             14987..16114
                     /locus_tag="PLL28_06615"
                     /inference="COORDINATES: protein motif:HMM:NF027711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5005 domain-containing protein"
                     /protein_id="HNF69031.1"
                     /translation="MKKSIASQLLLSLLTVMLFATCGEIVPDKEALTQSPDDESMMMT
                     GVTTKPANKFNNMFNRYGNGWTGGDATYSVKLPDGRTVWMFGDSFLDTVYADNTRPLT
                     SPLIRNCFMVQNGTNMTTLCGNNIDDPEALVNTADPANEWYWPGDGTVIGDTLYVFMM
                     YFVRTGGGGFDFAYQRTDLVTFSLPTITEVNRTTVFTNEDIMWGADILEDGSYIYVYG
                     PESFTFTKYPHVMRFPAGNIRGTKEFWNGTTWVTTEPATTVGRMKRTNGLNIDVSAQF
                     AVFYSGGKYRMVTQDVLFGPNIYSWEASNPNGPWKIKQTVYTTPETGGDIWTYNAWVH
                     PQFTTADGYILLSYNKNSLNFLDLFGDATIYRPQFVYFKYL"
     gene            complement(16287..>17625)
                     /gene="creD"
                     /locus_tag="PLL28_06620"
     CDS             complement(16287..>17625)
                     /gene="creD"
                     /locus_tag="PLL28_06620"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HNF69032.1"
                     /translation="TMQENPSPGAFEQLNNWFTRSITVKLIIIVFLALILLIPASMVQ
                     GIINERESKQSYAAESISNEWASEQNVAGPVLTVPVAVINPDGSKQWTNYVHLLPDDL
                     VIDGNVAPQSLHRGIYDAVVYTTALDIKATFDLSKLNSEIQDIKGTPDWQKAYFAVGI
                     SDLRGVKDNILVAVNNNSITAKAGVAKESPIHHGVSVPFKDVKIDSNGKFTVQYKLAL
                     QGSQMLEFQPLGNTTNIEIRSKWTNPSFTGAFLPDERVINDQGFAANWRVLELNRNFP
                     QTWKNDEYNIGLDESAFGITLMTGPDDYQKSMRSVKYAILTIGLTFLLFFLFEIMNKR
                     RMHPFQYILVGLALCLFYILLISLSEQMAFNLAYIISSMTIILMITLYSRTVFPKWRS
                     SGILAIILIALYTYLFVTIQISDYALLLGSLGLTLMLAVTMYATRKIKWYGNE"
BASE COUNT         5559 a         3594 c         3331 g         5141 t
ORIGIN      
        1 ttttgcttcc cgaatttagt agttttaatt gatactgttt tcgggggagc cttcatatga
       61 actatatcgt ttagttgccg ccacttcgca aagcttctaa acgttacctg taattttatg
      121 aaacgacaaa cattcatcat cctaactatt ttattttcct tgacagcatg tagtcaaaag
      181 gacaaacaaa ccatttctac acctcaaact ttggatgatg gctggaaaat atccgaacct
      241 gacaatgcgg gttttgacaa acagaaactt gaaaaattaa tcaggggcat ctcaagcgaa
      301 aatccaaaac tgaatagttt ggtaattgcc aggcacggta aattaattgc tgacgaatac
      361 ttcaacggtt acacgcccga cagtcttcaa aaaatttggt cagttacaaa gtccattaca
      421 ggaactatta ttggaattgc cgtagacaaa ggacttcttt cagaaaaaga cagcatatac
      481 aaatacctta ctgactatat tcccaatgca aattcctccg aaggagcaat cacgattgaa
      541 catctaatga caatgacaag cgggtttgaa tgggttgaat tgggcggacc aaaatcagcg
      601 ggatttcaac ttgcatattc taccgattgg atagcgttta ccttaaatca accgcatacc
      661 aatgttcccg gaacgatatt taattattcg acaggaaatg ctatgctttt agcaccaata
      721 gtcaaaaagg caacgggaca acaagctaat gaatttgcca aagacaattt gtttcttcca
      781 ctaggtatta caaattatga gtgggacaca caaagtgagt tttggactaa aactcaaagt
      841 ggagaattac cgggagcgaa acaaccaacc gacattgatt ataaaaaacc attttcagac
      901 tttactaata cgggttcagg tttacgaatg agatcaagag acttatgcaa aattggacaa
      961 ctgtatctta acagaggaaa atggaatgaa aatcaaattg tcagcgaaaa atggattaat
     1021 aaatctacac aatctcattt tggtaattca gactatggat acctttggcg acttatgaat
     1081 tttcaaaata attcttgcta ttatgcaaca ggatttgggc ttcaaaggat ttttgttatc
     1141 ccaacacttg acctggtagt ggtaatgaca cagcaacatt atgaaacaat gcctcaagga
     1201 gaaaaatgga taaatcagtt tttgaccgaa ctgttggaaa cgataaaaga atgaaaaaac
     1261 tgcagcaggc agcaaaaatt tttaaaggac agatgataaa acgacaatca actatatcga
     1321 cactatttct cttgttgacg attatctatt cgtcctgcaa tggacaagta aatacatacc
     1381 ccaccaaaga caaacagcta aaaatcatta cgaataactt cttgcccgaa cttgctgaag
     1441 aatataagta tgttcaatgc ggcctgcagg atacgaaggg aaatatgtgg tttggcactg
     1501 ccggcaatgg catctatgtc tatgacggag actcctttgt aaatttcaca cataaatcct
     1561 ttattaactt ttccctcaaa gaagatttga atcataatga tattctttgt tgcaaggaag
     1621 atatacatgg caatatctgg tttggaacaa gaagaggatt aattcgatat acgccaagta
     1681 aggagaaacc agaagccaaa gattttactc ttattctaat tccggcaaat actatcaaca
     1741 attcttcccc caaccgcctg ccatatacgc tacagtccgg ggataatttt gtatggagca
     1801 taatgcaaga caagtcagga aaaatctggt tcggtacaag cagaggtatt tatgttcaca
     1861 atccttccac agataatgat agtattggac cgcttttcaa acgcttttta gatcaagaca
     1921 gcctcaccaa caaccagcat ctgcaattac tcaacgtaac gagtatgcag gaggacagaa
     1981 acgggaacat atggttcgtg tcacctaatt ggacggcaaa caaaggcgaa ggcattgtac
     2041 gttatgatgg aaagtcactg gttaccttta ctcctgacag tacatattcc ttccgatcaa
     2101 ttattggaag aaacaacgga gacttgttgt ttctaagtcc atttaatggt gtttattcgt
     2161 atgatgggaa aacattttct aatctcaccg aaaatattgg aattaaaaac gacacactta
     2221 tgtcaataat ggaggataaa aacggaaacc tgtggcttgg acacatcagc gaaaatgtta
     2281 aaaatggtgg agaaggtgga ttatggtatt atgatggtaa atcattaaaa ttatttacaa
     2341 ttaatgatgg actgccccac aatcatgtta tgtgtattgt atctgacaaa gaaggaaaca
     2401 tatggtttgg gacaagaaat acaggcctgt gtcgttatga cggaaaaaca attacgaact
     2461 actcagacta acaggacata aaactgttgc aaacacgagt ttagcttcag gcgagcccaa
     2521 atacaaactt gaaactttgt cctcctaatt ccgctcgagc gcaaaacccg acaacgtcat
     2581 gtgcaacttt taaccaaagc caaaatctct ttagtgaaaa aggtaaagat aaaatcgcat
     2641 atagaacttt acgaattatt gccagagaat gagagaatca ttgttgatgt tcttaggcaa
     2701 atagtgaaag cgactcttcc ttcaaactgc aaagagaaaa tctcatttag cgttccttat
     2761 ttttacggca acaaaggaat atgcattatc tggccggcaa ccattcctcg aggaggtatt
     2821 aaaaagggtg tcttatttgg cctctggtat ggaaatagac taaaagatcc ggacaattac
     2881 ttaacccacg gcacaaacaa gcagattttc tataaaatct acaactcgcc ggaagaaatt
     2941 gacttcaaaa aagtagtaaa gcttttaaaa gaggctgtag ttctcgatac tcaatggtag
     3001 tatcataaaa actgcccata tcaaaagcat tgttgcaagc agagtttgac caatcatttt
     3061 cagaagttgt gcgatattca tcctttgctt caggctgacg tgcacacaat aataattttg
     3121 tctaacttca tacctggcaa caattttgag atggccggcc gggcgggatc agctataaat
     3181 tctcgtcctg tttacaatgc ctgcacgttt gtatccattt tctgttcgaa tatcaatcca
     3241 ataaactaat tttaatcaaa ttcctgttca gatgcaacat gcttaataac catacgctac
     3301 caaaccatac gaacgggaac ctaaaataaa tggaaaatat tataagacat tttgacaagt
     3361 accttccatt aggcgacaac gaaaaagaag ccttaacaag tcgtttgata gaacgcaaac
     3421 taaaacgtaa acaatacttg ttgcaagaag gtgaaatttg caggtatttt agttatgttg
     3481 tagaaggttg tattaattta caaattacac taaccaccag ttttctcgat gttacacttc
     3541 gggtataatg gcgcattcag ttaatgctta actttgatca tgcaaaggtt acttctatga
     3601 aaaccctatc catcaacagg ttaatacttg caatcggcat accggcttta atcattatgg
     3661 tattattcgc gcttaccaag acggagttgc ttattgaaaa gccaacattt ggtgccttcg
     3721 ctatctcctt cgatctgttg atcacagttc catgtatata ttatatcatc atcagaaata
     3781 gtaagatctc tccacttacg ctatttccta tggtcatcgc aaccatggtg attgccacat
     3841 tgatacttcc gcgcgaaaac caatattatc tggacctgtt caaacgatat gccattccat
     3901 tgatcgaact tggttttatc ttactggtta tcagagggat ccgcagatct gtcagatctc
     3961 atcagatggc tggccaaatt aaatacgatt tctactccgt tttacaacta gtttgttacg
     4021 atttactgcc taaaagaatt gccggcattt tcattaccga attggccgtg atctattacg
     4081 ggttgattca atggaagcaa cgacctattc aatcaaatga atttacttat cacaaatcga
     4141 gcggaacacc tgcaatgatg ggaatgatcc tgttcatact tgctgttgaa acatttgcta
     4201 cccatttgtt gatcgcaaaa tggaacggaa ctgctgcatg gattttgacc ggattaagtg
     4261 gatatgtgat tattcaactt cttggattta tcaggtcatt atctaaaaga cccatgttca
     4321 tcatgaataa catcttgttc attcggtatg gcatactagc agaggtttct atcaatatag
     4381 atgacattaa tagtgttgag ttttcaaatc gcaaattcaa agacaaccat cacaccagaa
     4441 agctttctgc tttcggcaac cttgaatcgc acaatgtgat catcaagcta aaaaatccat
     4501 acacgctttc gcgcatctac ggaatcaaaa agcagtttac cgaactggta ctgttagttg
     4561 acgataaaca aggcttcaaa cacgccatcg aagccgctaa gtaacctcac acaccctctc
     4621 ccccctcctc tctcctttct cctctctcct ttctcctctc tcctttctcc tctctcctac
     4681 caatgacttt tgtcaccatt caaggtaacc ctcatcatta taaaaagtat ttaattactt
     4741 ttattttgca ttactaaaca ttctactatg agaaatctta acattattgc catattgatg
     4801 gtaatgtctc tattgttcgc ctcctgtggc ggcaagaata cacccgctgc aaacggcgaa
     4861 gccccgaaaa gcatgcttgg tcccaagact gaaaatgaga aaatgattgc cgatggtcgc
     4921 ggaatcggcg aagtgaaatc tgttaccctg aatacccccc ttgagcaaga aagggtaaaa
     4981 cgtggtcagg atatatatga catgaaatgc tctgcctgcc acaaactcac cgatgagcgc
     5041 tttgtaggcc cgggttggaa aggagttact tcacgtcgca aaccggagtg gatcatgaat
     5101 atggtcacta atgtcgacgt catgcttgaa cgcgatgccg aagcccaaaa actgctcgag
     5161 ctatgtctca cccgcatgcc aaatcaaaat gtttcgatcg gcgacgcccg cgatattctt
     5221 gagtttatgc gcagcaacga tggagaaaaa taaattgtat ttgatcattc tacattaaac
     5281 cataacttac tatgaaaaac ctaaccctta tgctattggt aaccatgctt accatagcgg
     5341 gatgtaaacc caaaacgggt accagtgttg catcaggcga tgctgcttca aaagtatatg
     5401 tagcacccgg tacatatgac gaattttaca atttcgtatc cggcggtttt agcggccagc
     5461 ttgccgttta tggcattccg tcaggacgac ttttccgcga aattcccgtg ttctctgaag
     5521 atcctgaaac tggatgggga tacaccgaag aaacaaaacc catgctcatg acttcacatg
     5581 gttatgtacc ttgggatgac ttccatcacc ctgcgctctc tactaccaat ggcgtgcacg
     5641 acggcagatg ggcatttgga aatgccaata atacaccgcg tattgctcgt atagatttga
     5701 aaacattccg cactgctgag attatcgaaa ttccaaatag ttccggtaat cacagctctc
     5761 cctttattac tgaaaataca gaatacacta tcgcaggtac tcgctttagc ttgcctcctg
     5821 attatgaagg tgcaaacacc gatgtatcca tttcttctta taaggataat ttcaaaggtt
     5881 atctgagttt tatcagcgtt ggtaaagaaa caggaaatat ggatgtagcc ttccagattc
     5941 agataccacc attaaatacc gaccttgcaa gaggaggaaa aggcccatca cacgattggt
     6001 tcttcttcag ctgctacaat tctgaaaaag ccttcacgtt acttgaagta aatgctagtc
     6061 agaaagataa agattttatt cttgccgtta actggaaaaa agctgaagaa tatgctaaag
     6121 caggaaaagg ccagcatcaa aaagtaaaat atacccacaa cacatatgat gaaaaaacgc
     6181 attctgccac atcagaaatt aaaacagatg tgctggtttt agatccaaaa gaattaaaag
     6241 acgttttata tcttattcct tgtccaaaat caccacatgg ttgtgacgtt gatcctacag
     6301 gtcaatacat agttggatca ggtaaacttg ctgctttgat tcctgtattt tcattcgaaa
     6361 aaattcaaaa ggctattgct aataatgatt ttgaagcaga atatgatggt attccggtat
     6421 taaaatatga aagttgttta catggtgaag tacaaaaacc gggtttaggt ccattgcata
     6481 cagaatttga cgatcaagga aatgcctata catcattctt tgtttcgagt gagatcgtta
     6541 aatggaatgt aaaatcactt caagtacttg atcgcgttcc tacctattat tcaattggtc
     6601 acctttgtgt tcccggcgga ccaacagcca aaccttgggg taaatatgtg atcgcgtata
     6661 acaaaatcac aaaagatcgt tatctgccta ctggacctga attaactcaa tcagctcagt
     6721 tatatagcat tgagggtgat aagatgaaat tattgctcga cttcccgaca acaggtgagc
     6781 cacactatgc cgaagcaatt cctgcatcat tggtacaacc taattcaacc aaaattttca
     6841 agattgagga aaacaatcac ccatatgcag caaaaggcga aaaagaagca aaagttttcc
     6901 gtgagggaaa tattgttcac gtggtaatga catcaatacg ctctcacttt gtgcctgata
     6961 atattgaagg tgttaaaatg ggtgatgtag tatacttcca tgtcaccaac cttgagcaag
     7021 actgggatgt accacatgga tttgtagtga aaggagctaa caacgccgaa atgctgatca
     7081 tgcccggcga aacccaaaca ttgaagtggg aacctgatcg cgttggcgta ttcccattct
     7141 attgcaccga tttctgctca gcactgcatc aggaaatgca aggatatatt cgcgtatcgc
     7201 ctgccggttc atctgtacca cttactttca gtacaggcaa aacagcagca gaaccaccgg
     7261 caaaataaat tgagcaagaa ttaccatacc gattaaacac atattatgaa aaagctatta
     7321 tatattttac cattatgcct ggcattgcaa ttcggttgca ataatgcaga gagcacagaa
     7381 gcaagtggta acaacgatac aatgaatgca caagccggcg gacaggaagc tgttcaagac
     7441 gacgtttcac aaaaggatgt tgtaaaaata gcagttggct caccggatca taccactttg
     7501 gtagcagccc ttaaacaaac agaatatgtc aacgatttga gcaatgccgg tccatttact
     7561 gtattcgcac caacaaatgc cgcatttgat aaattacctg cgggaactgt tgatgacctg
     7621 atgaaagatg aaaaccaggc tgccctgcgc aatattctgg aataccatgt tgctcttggt
     7681 acctatcgca aagaatacct gaacaatggg caaacaattg gcatggcaaa tggtgacaat
     7741 gttaccattg gtattaatgg cgatgatgtt accgttaacg gcaacaaggt gctcgctatt
     7801 gttcccgcct caaatggaat tatttatatt gtggatggcg tattactgcc tcctgctaaa
     7861 aaataatagg ttgcaatgtg caaccggtct taatggttga gtgtttggaa aatcacaacc
     7921 gggatgcaat accgtatccc ggttgttaaa ccacggcata tgaaaaagat tcacaattat
     7981 tccgcaatag ctgtagcaat agccgcactt tgttttctgg caacctttaa atttcctttt
     8041 tgggtaattt atctcaccgc accacaatat ccggaggggc ttacactcta tatttggtta
     8101 cacaaggtaa gtggtgatgt agaaatcata aatggattga atcactatat tgggatggca
     8161 attattgatg aaactcaatt ccctgaattc gatattctgc cctacgcaat aggatttatg
     8221 gttggagcgg gattgctaat agccttttta aggaaaaaga aattgctgtt cttctatacc
     8281 attttcctgg tgttattttc tatccttgct atttacgatt tctggcactg ggaatatcaa
     8341 tatggtcaca accttgatcc ggacgctgca attcaggtac cgggcatgag ctatcagcca
     8401 ccacttatcg gcttcaaaca attattaaac tttggtgcat actcattccc tcacgggggt
     8461 ggattctttt atatctccgg tgtagtgatt tgttgcatcg catccattct tgagtggagg
     8521 cgtggaaaaa aacagttgac tcctaaaata agataacatg aaatcattat catatttaag
     8581 ctttatctgc ataatgctat tgagtgcttg ctctgtcaaa cctcacccaa tcaatgaagg
     8641 ggaggatgcc tgcgcatatt gcaaaatgac agtagccgaa aaaaaatacg ctacagaaat
     8701 agtaagcaat accggtaaaa tatatgtttt tgacgatatg cattgcatga aatcattcat
     8761 taaagaaaac atagtacctt caggcgatat taaactcatg atggtaagtg atttctataa
     8821 tccagaaaat cttatccctg ctgataatgc actgctattt acatcagaat cattgcatac
     8881 accaatgaac ggcaaaacag taggccttgc cgatagcggc gccctaaaac gtattcaaca
     8941 ggaaatgcaa ggcaattcgc ttaccattca acaattgctg caataattaa ttatatttgg
     9001 ttatgctgcg ttttatatgg atttggatac tcattcattg ctcccttggc gcacagagca
     9061 aaaccataca tgtaaacaac cataaaggct tagcaggtgt acaagatgcc attaatatcg
     9121 ctcaactcta tgatgaaatt ctacttgaag aaggtgttta taccggagaa ggtctatata
     9181 tcaataaacc aataatactc cgcggtaata ataatgttat attggatgga ggcgaaaagg
     9241 ttaccattct tacaatcgag ggcaccgctg cccttgtgga aggactaacc ttccgaaata
     9301 ccggttatag cagtgttgaa gagcgttgtg cgattaaact tctctcagca agaggctgca
     9361 tcatcaccaa taacaaaata gaacattgct gcttcggcat catgctgatg aaatcaggtc
     9421 atagcattat aacagataac aacatcaatg ccacaaacat ccacgaacaa agaagcggca
     9481 acggcatcca tggatggcaa agtgatagtc tcacaatcag aaataataac attcaaggtc
     9541 atcgcgatgg tatatatttc gaatttgtta catgcaccgg tgtttacaat aataagagca
     9601 ttaataattt gcgatacggc atacacttta tgttctcgaa taatgatgag tacatcggaa
     9661 ataccttttc agacaatggt tcaggagtgg ctgtgatgtt ttcacatcat gtaaatatca
     9721 gaaacaatgc cttcgataat tgcatgggtg gcgcttccta cggaatcctg ctaaaagaga
     9781 taaatgattc acaaatcatc aataataaat ttcgacacaa tacaaatggt atctttatgg
     9841 aaggtacaaa tcgcattacc ctccaacgaa atttattttt aagcaacgga tgggcttgca
     9901 gagtacaggc cagttgttct tcaatatatt tgaacgaaaa taatttcatt ggaaactcct
     9961 tcgatatagc aactaatgga tcgcttagca tcaacaattt caatggcaac tattgggata
    10021 aatacgaagg ttacgatctg gacaaaaacc atattggcga tatacccttt tctcctgtta
    10081 gtatgtatgg tgtaatagtt gaacgcatgc cctataccat tttactctat cgcagtctta
    10141 tggtgtattt actcgaccga tctgaaaaag tgcttcccgg aatcaccagt gaacaaatta
    10201 aagacaagca gccgctcatg catgcggtaa acttatgata caaattgatc atctgagtaa
    10261 atcctataaa agtgttcatg cactcaacga tctgtcactg accttcactg ccgggcagtc
    10321 aatcgccctg accggtccaa acggttcggg aaagtcgaca ttaatgaaat gtctcttagg
    10381 attggtgttt cccgataaag gaaatattac tgttaataac ctgcgggtag ataaaggttc
    10441 tgagtatcga agatttattg gctatatgcc acagattgtt cagtttccag aaaacatgcg
    10501 cgttcggcag ttatttaaaa tgatgcgaga catcaggcag catgatggaa attatgacga
    10561 agatctctat actgccttcg acatacaagg catggccgat aaatatctgg gttcactttc
    10621 aggaggtatg aaacaaaagg taaatgcagg tttagccctt ctcttcaacc ccaaaatcat
    10681 tataatggat gaacccactg caggtttaga tccattgtcg gcagaattac taaaagacaa
    10741 actacgcaaa gaaaaagaaa aagggaaatt aataattatt tcctcacaca tcctctcaga
    10801 ccttgaagaa atcaccaacc atgtattatt tttgatggat ggtaaagtaa tttgtaataa
    10861 gtcattaccg gagctcattg ctgaaagtgg cacagaaaat cttagtcgcg ctattgcaaa
    10921 attaatggat ccaaaaatcg ccggcaatga aactcgttaa atatgtattc tgggatatcg
    10981 cacgaaataa aatcctaatc ggatatgcca tgtttttatt tgctgttagc tttgggttat
    11041 tctcattcag tgacgatccg attaaagcaa caagcagtct tcttaatatt gtattaatcg
    11101 tcttgccatt attcagtgtt atattcgcaa ccagctatta ttacaatgct tatgaattta
    11161 caacactggt gcttggtcaa ccaataaatc gattgcattt attcttttgt cagtttgcag
    11221 gcgtttgtat tgcaatgaca ttggcattta tcatgggttg cggtatcccg attataatgt
    11281 atatgccaaa tataataggc atcatgcttt tgttaagtgg catttcgctg acattggtat
    11341 ttatttcact cgcctttatg gcaggagttt tggcaagaga caaggccaga ggtataggta
    11401 ttgcacttct tctctggttt tactttgcat ttctttacga cgcgcttgtc ctgatgctcc
    11461 tttttgcatt cagcgattat cccatggaaa ataccatgat gacactggcg tttttgaatc
    11521 ccgttgacct gataagaatc attatgctca tgcaactcga catggccgca attatgggtt
    11581 ataccggtgc cgtattcaat aaattgttgg gatcaagctc aggtacaata ttgtgctttg
    11641 ctgctattct tgtgtggatt gcactgccac ttacaatcag cgcgcttagg tttcgaagga
    11701 aagacattta gggcttctac ggtaatcttc ttaccttttc gcaaccaagt aggacaattc
    11761 cctatcttta tactaaacca cctactatga aaacgatata cattgtttta cttgcgatta
    11821 ttccctttac tcatattcag gcataggatc ccatttcatt tccaaataat aatgcaacct
    11881 ggatgacctt gcgcggctca tgggatgatt tcggaaactc taatttatac ggtaatatta
    11941 tttcaccaac cgaagatatc ctgattgatg gtcttgcctt ccaaaagcca tcgctcacac
    12001 ttaattatac cacttcatcc attttccccg gcaccacttc agcagatgac acttatatac
    12061 cactggacac aattgcagct attcgcgtag aggatgaaaa ggtttatttg cgtatttcga
    12121 ccctatcgtt tctgaaatga ccatcaccga ttatgcacca caatacggag gtcttccatt
    12181 agacacaaca tttatattat acgattttgg attgcaggta aattataccg ttttctatcc
    12241 aattgatcat acccctgtta tgaatgaatc catgattgat tctgttctat taaatgatgg
    12301 catttaccac caacgatatc atttttacga aacaggtgcc atagagcagg tatggctgga
    12361 aggcataggc agtgaaaatg gcatatttgg cgctttcctt ccgattgctg catacatgaa
    12421 cggttccagc tggcttgaat gtttcaaacg tggtgacact tatgtgtatg ggaccgacag
    12481 ttgtgatttc aatctgcatt aaacaacggc aattcatgaa tctactcccg aaacgataag
    12541 ctgctacgta tgggaaaatc agatatatat taaaaatcta ccggcaacta tgcaggcgcg
    12601 aattcactta ttcaatgtac aaggacaatg cgtatttgat agtgaaatac cggattcaaa
    12661 aattatttcc attcccaatt tgataacagg catatatacc gcagtcattc aggttgaatc
    12721 agggccgact ttttcgcaac agttgaagct ttaataagta attcaataaa atctgctgct
    12781 tgatataatt tccaatgaaa ggaaaattct taatagagca gtatgcatgt cttgcagcag
    12841 gcttcttgct cttaaataaa caaagtgaca gccaggtggt ttatacagat gttgagccgg
    12901 attggatatt tcaagatgat ggcgacttct catatctgga tatgaatgat gacggtataa
    12961 atgatttcgg ttttctgaaa tcaaatcaca tcatttcaac tctttcttcg ccgggattaa
    13021 atacaacatg gtactataga attgcactat gggttggtcc agccatggta ggtcctgata
    13081 ttgccggcat ttcagcccac gccagttcag ctgcaggcac ctattgtttc ccctatcctc
    13141 tggaaaaaag caatctcatc aataacgatc taagttttca gaattgcgga tttcaacttt
    13201 tagcatctaa aaccgcagaa gtgtattatg gatatagcta tacatttttg ggaataggag
    13261 actggcatat tggaatagga gaattcaata atttaaattt agattcaacc cgctatatag
    13321 gggtccggtt tgaagatgag gatgactgtc tgcattatgg ttggattcga tgcgctgtta
    13381 tggattctat cgggaaactc gtggttatgg attatgcata tgaaatgcaa tgcaataaaa
    13441 caattgcggc aggaaatact gtttcatatg tagatattaa ttcagaaaca actgattcag
    13501 ggatatctat ttacgctttt gatggtaaaa tttattttca tggaaattcc gcgggtaaaa
    13561 tggtaattac cgtctcagac attaccggac aaattgtagc tcatcagtgc atttccggaa
    13621 acgataaaac aattaatatg aattttgaac ctaaaggcat ctatgtggta aatgttgtct
    13681 gcaatggaaa atattttaac aaaaaagtag tcatagattg atatttaata aaaaaaaata
    13741 tgaagaggag atgaagaata acggcatgag gataagttaa atactgcgaa atatttatcc
    13801 cttgaaaaat aacaatatcc actactgctt ctttgtaaat ctgaagcagg tgacaaaata
    13861 tagtgcgacc gatttcatgt tatgtttatg atgccccatc atgcttcact ctatgcatga
    13921 tcaacactgc tgttgctaat aatatgcatg ctgtaatttg atgcgctaca cccagaaaaa
    13981 cgggaatttg taagctgcca ttcagcaatg tgagcacacc taatacacac tgtagtacaa
    14041 caagcagaac catcgtaaga ttcagctgct tcaatttttg agaaccggta tgtcgattgc
    14101 gataccaata aataattacg gcagttgcca caattaatgc aaatgtgcga tgtatgaaat
    14161 ttatttccgt tgtcagctgt gtcagttttt ctgcttgcga taaattagaa aatgcgccat
    14221 caggcaaaaa tgcaccattc atcaagggcc atgtattaaa taatctgcct gcatcggtgc
    14281 ctgccataat tccacccaat ccgatttgaa taaatatcaa tgctgtgatc caattcatgt
    14341 ttcctttagc tattggctta tgcgcaattt tacaatacgt atcttgtgcc aaccaaatta
    14401 aatacatata taatgtcaat gccagtaata aatgtatcgt gagtttactt ggatcaaccc
    14461 aagggtctcc atctaatcca cttgctacca tcacccagcc tacaaatccc tgcaatcccc
    14521 ctaaaacaaa aacgaatatg agttttttct tccacgaagc atttaattgt tttttaatcc
    14581 aaaaccagat gaaaggaatt aaaaatacaa agccaagcgt gcgagcccac aagcgatgaa
    14641 taaattccca ccagtaaata cgcttgaaat cacttacggt aaaatcggtg ttaacttttt
    14701 caaattgcgc cgtttgctga tatttggcaa attcctcctc ccatgccgat tgcgatgttg
    14761 gcggcatact gcccattacc acatcccatt ctactatcga caagcccgat tccgttaatc
    14821 gggtgatacc acccaacata acctgaatca atatcatcac cacacccgtc caaagccaga
    14881 ttcgaacatt gcgcatatcg gatttttttg caaaagtagc ttacatcagt tcaattaaca
    14941 catatttatt tcctttgttt aactttgata gataagaatc acaccaatga aaaaatcaat
    15001 tgcctcccaa ttactcttaa gcttgctgac tgtgatgctt ttcgccactt gcggtgagat
    15061 agttcccgat aaggaagcgc tcactcagag ccccgacgat gagtctatga tgatgaccgg
    15121 cgttactacc aaaccggcaa acaaatttaa caatatgttc aaccgctacg gtaatgggtg
    15181 gacaggcggc gatgctactt attcggttaa attgcctgat ggcagaactg tttggatgtt
    15241 tggcgattca tttctcgaca ccgtttatgc cgataatacg cgtccattaa caagcccttt
    15301 gattcgcaac tgcttcatgg tacaaaacgg aaccaacatg accacgctgt gcggcaacaa
    15361 tatcgacgat cccgaagcct tggtgaatac tgccgaccct gcaaatgaat ggtattggcc
    15421 cggtgatggc actgttattg gcgatacatt gtatgttttc atgatgtatt ttgttcgcac
    15481 cggaggcggt ggttttgatt ttgcatatca aagaacagat ctggttacat tttcacttcc
    15541 gacaatcaca gaagttaatc gcactaccgt atttacaaat gaggatatta tgtggggtgc
    15601 agatattctt gaagatggca gctacattta tgtatacgga cctgaatcat ttacgttcac
    15661 aaaatatccg catgtgatgc gctttccggc aggaaatatt cgaggaacaa aggaattctg
    15721 gaatggcact acttgggtaa caacggagcc tgctacaaca gtaggcagaa tgaaacgcac
    15781 taatggatta aatattgatg tttctgcaca atttgctgta ttttattcag gaggtaaata
    15841 tcgaatggtt acgcaggatg tattgtttgg cccgaatatt tattcctggg aagcatcaaa
    15901 tcccaatgga ccatggaaaa taaaacaaac tgtttataca acgcccgaaa caggtggcga
    15961 tatatggact tacaacgcat gggtgcatcc acaatttact actgccgatg gttatatatt
    16021 attgtcgtat aataaaaaca gtctcaattt tcttgatctg tttggcgatg ccaccattta
    16081 ccgaccgcag tttgtttatt tcaaatacct ctaaccaaaa ccattaaaaa aaagggatct
    16141 acaatatgca gatccctttt tttatggggt ttatgtaata cggagggttg gtttcacgca
    16201 gaggcgcaga gacgcagagc tttttccttt ttcctttttc ctttctcctt tttcctttct
    16261 cctttttcct ctctcctttt tcctctctac tcattcccgt accatttaat tttcctggta
    16321 gcatacatcg ttactgccag catcaaggtc agtccgagac taccgagcaa taaagcataa
    16381 tcgctaatct gtatggtaac aaaaaggtag gtatacaatg ctataagtat gatcgccaat
    16441 attcccgatg atcgccattt tggaaacacc gttcttgaat ataaggtgat cattaatatt
    16501 atggtcatag aagaaataat atatgccaaa ttaaatgcca tctgttccga taatgatatc
    16561 aacaaaatat aaaataaaca tagtgccaat ccaactaaaa tatattggaa aggatgcatg
    16621 cgacgtttat tcataatttc gaacaggaag aaaagtaaga atgtgagacc gattgttaaa
    16681 atggcatatt ttacacttcg catagatttc tgataatcat caggtccggt cattaatgta
    16741 attccaaagg ccgattcatc caaaccaata ttatattcat catttttcca tgtctgcgga
    16801 aaatttctgt tgagttccaa aactctccaa tttgctgcaa acccctggtc atttatcacg
    16861 cgttcatctg gcaaaaatgc accggtaaaa cttggattgg tccatttact tctgatttca
    16921 atattggtag tatttcccaa aggttggaat tctaacatct ggctaccttg taatgctagt
    16981 ttatactgca cggtaaattt tccgttggaa tcaattttca catccttaaa tggcactgaa
    17041 acgccatgat gaataggcga ttctttcgca acacctgctt ttgcggtgat actattatta
    17101 ttaacagcta ccagtatatt atccttcaca ccacgcagat cagaaatacc tactgcaaaa
    17161 tatgcttttt gccaatccgg tgttcctttg atatcttgta tttcgctatt gagttttgat
    17221 aaatcgaatg ttgcttttat gtccaatgcg gtagtataaa ccacggcatc atatattccg
    17281 cgatgcagac tttgaggtgc aacatttcca tctatcacca gatcatcagg caataaatgc
    17341 acataattag tccattgctt actgccatcg ggattgatca ccgcaaccgg aaccgtcaac
    17401 accgggcccg caacattttg ctccgatgcc cattcgttac taatggattc ggctgcatac
    17461 gattgcttcg actctcgttc attaataata ccttgcacca tcgatgccgg aatcaacaga
    17521 ataagcgcca aaaaaacgat aataataagc ttaaccgtta tcgagcgtgt aaaccaattg
    17581 tttaactgct caaatgcgcc gggagaagga ttttcttgca tagtt
//
LOCUS       DAOQHY010000060        17453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1515_length_17453_cov_5.938959, whole genome shotgun sequence.
ACCESSION   DAOQHY010000060 DAOQHY010000000
VERSION     DAOQHY010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 17453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17453
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1515_length_17453_cov_5.938959"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..665
                     /gene="purQ"
                     /locus_tag="PLL28_06625"
     CDS             3..665
                     /gene="purQ"
                     /locus_tag="PLL28_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNF69033.1"
                     /translation="MHILRNVMGQEVIELFHKEHDLRGLTTADCVMLPGGFSYGDALR
                     TGAIARFSPIMQAVIDHCNKGGYAWGICNGFQILCEAGLLPGVLLENNNQQFICKNVF
                     LSADNNASRINATLPLQHALKIPIAHGEGRYYAEARELEALEANNQVIFRYCDDHGHI
                     TERANPNGSLNNIAGVCNAGRNVFGMMPHPERAAEERLGNTDGRLIFESFLALTEATA
                     AR"
     gene            766..3183
                     /locus_tag="PLL28_06630"
     CDS             766..3183
                     /locus_tag="PLL28_06630"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNF69034.1"
                     /translation="MKRLLIALCSLFLFTNVKAQILEPVKWEVSSTQTGVNEYVLIFK
                     ATVQEGWKIYSHSVPENGPIPTSLIFENLPAGVQLVGEMEELGRMEEQQEPLFDNNII
                     KFYHKRLTLRQAVKISGNAKITGYIDYMSCNDQTCTALSYDFSFDLEGQSGFVQVPGS
                     DYANVKYPVTWKIGSEKLNDNEYYITYSATIVPTWKLYAQTSPEGAARPMKFIFENDG
                     QGYTRIGGVEEIGHMEEKKEQLFENKTVRFYHDEVMMRQRIRIDSANANIIGYIDYQT
                     CDATQCKFMQKDFNLALSATGLIETGSTTPIDTGGMKFGAFAVDLKYAENAMCEGEVN
                     AQITNATGKGLFSTFILGFIGGLIGLLMPCTFPMIPFTISFFTKHAGNRRKGVMNAAF
                     YGLCIVLVYFIASLPFLLFGAGGDQLNEFSTNNIVNLVFFFVFILFALSLFGLFEIKL
                     PSKWANKADSASDVGGLLGIFFMALTLCIVSFSCTGPILGSLLANSIKDGGVALTMGM
                     LGFGTAFALAFTLLALFPQLLGNLPKSGGWLNEVKIVFAFIELAMAVKFLSNVDLAYH
                     WGYVKREIFLGIWTLIALLIVLYISGVIRFPHDDKKVKWSLGRVSFLITFLFFTIYFG
                     QGLRKYGEVGLLSGFLPPQWYSIYEYGTECPLGLNCFHDLDEGIAFAKEHNKPILVDF
                     TGYACVNCRKMEENVWPEEEVFNIINNDYVLVSLYVDDKEALRLEDQYISAATGQKVR
                     TIGNKWSDFQSSQFNTNSQPYYVLLAPDGKMLNQPRAYTPNVSEYRGFLECGIETFKN
                     MDKIAAK"
     gene            3392..3697
                     /locus_tag="PLL28_06635"
     CDS             3392..3697
                     /locus_tag="PLL28_06635"
                     /inference="COORDINATES: protein motif:HMM:NF024759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNF69035.1"
                     /translation="MPESAYEAALSHKLIIAGLNVRRQVSLPLVYDGVKMDIGYRIDL
                     LIEDLVIIEIKSAENLTKVHHKQLLTYLKLSGHKLGILVNFNCSDITKNIFRKVNYL"
     gene            complement(3988..5814)
                     /locus_tag="PLL28_06640"
     CDS             complement(3988..5814)
                     /locus_tag="PLL28_06640"
                     /inference="COORDINATES: protein motif:HMM:NF013560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HNF69036.1"
                     /translation="MRKLLTLSLLIPIALISCKPKEKAEDNSAIQKEVQTYLDGYNAE
                     YQKLVTASSEGQWILQTHIVEGDTMASHNAAIADEAYAKFTGSKTNIETAQKYLAMKD
                     KLTAIQVRQLEYIRYLAGNNPETAGDVVKNRITATNAQVEKLFGFKYTLNGKPVTTNQ
                     LDEILRSSNNMNERLAAWTASKEVGVGLKDGLVELQKLRNESVTPLGYSDFFAYLASE
                     YNMTSEEMLELTRSFITDVWPLYRELHTWARYELAEKYNQPVPDYIPAQWLPNRWGQD
                     WTAMVNVEGLDIDPELKKHDAEWVMKQGEAFWMSLGFDALPQSFWEKSSLYPVSADAG
                     YAKNNHASAWHIDLDKDVRSLMSVEANTEWWSTVLHELGHIYYYMSYSNPDVPPVLRT
                     GANRGYHEAFGTMIGLASLQKPLLENQGLIKPGVVTNDTLKLLSEALDYIVHIPWGSG
                     VMTEFEYALYAKNLSPDQYNAKWWELVKQYQGIVPPSPRDEHYCDAATKTHINDDPAQ
                     YYDYSISNVLLFQFHDYIAKNILHQDPHATNYWGSKETGDFLRKVMTPGATVDWREHL
                     KASIGSDMSAKPMLDYFMPLMDYLKKQNAGRTYTLPEKPTFN"
     gene            6017..7030
                     /gene="recA"
                     /locus_tag="PLL28_06645"
     CDS             6017..7030
                     /gene="recA"
                     /locus_tag="PLL28_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNF69037.1"
                     /translation="MAEATNDKVKALGLAVDSLNKLYGKGTVMKLGESAVTAEEVIPT
                     GSLGVDMALGIGGIPKGRVVEIYGPESSGKTTLAIHVIAEAQKKGGICAIIDAEHAFD
                     KVYAEKLGVDVENLLISQPDYGEQALEIADHLIRSGALDVVVIDSVAALVPKGELEGE
                     MGDSKMGLQARLMSQALRKLTGTINKTGCCCIFINQLREKIGVMFGNPETTTGGNALK
                     FYASVRIDIRRIGQLKDKDDVIGNRVKVKIVKNKVAPPFRQAEFDIIYGEGISKIGEI
                     IDLGAESGVIQKSGSWYSYEGTKLGQGREGVKDILRDNPELALEIEKKIKAKLSGVPT
                     KED"
     assembly_gap    7138..7237
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7500..8096
                     /locus_tag="PLL28_06650"
     CDS             7500..8096
                     /locus_tag="PLL28_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69038.1"
                     /translation="MTTFKTRDEQDVIWFITFTCYRWLPLIETTDLYDDIYKWFDILK
                     SEGHSILGYVIMPNHMHMLMYFSKNKRTINTCMAEGKKFRAWEIIKRLKKQRASHYLI
                     QMEWVPEDHKNPNKQKFMAFEPSFDCKVCKSEERVLTKLNYIHKNPVNKGYHLAPSMT
                     AYKHSSAKYYHTGIHSAYPVEHYKRFIRPFNCNIDAPQ"
     gene            8195..9688
                     /locus_tag="PLL28_06655"
     CDS             8195..9688
                     /locus_tag="PLL28_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69039.1"
                     /translation="MNIKANCYTLLIALLTRIPAFGQLEAVDTISYPVDSEHSTYVFQ
                     NLGNDGYILMYKSNSDRVELSKYDTLNNETNHNSFEVVAHLRYAVYPDKSYSDYFDIS
                     ENNEVVNHRIDFKTLDVVTTKCSVDIKLKYKYQVSNNDCILLVFENYFKQEIYKVDIK
                     TGQVNRIISDLPEKKKKSFAYQQITVVPSTGEFFLFTNKQKFFKTYNLSAHLNNEGEQ
                     IGAYVLNPKPSKFYQNYTVVATPDNEYQIWCEMKYIKKLSDYGNPKISGLCQIVIDDE
                     SVVSRKSIPFRSFKNFYAFQKDNKSKNIDKNSLKYRMGHPTKVNMHTIQIKLVDDAVY
                     YAGEVYWPVYKTVVEQTVGSTAKPTKKMVLDYVIYSHAVIAKFDLEGNMIWNNSVELD
                     VDDTEAVLQINKYQINKNGVGVLLINEGAITLTKFDADGRIVSKYNTPCSAPICVDEE
                     KRKFNFFSLQYWYNDYMVYPEYTIDDDPSTERTEGYLNLYKYELIQD"
     gene            complement(9863..11077)
                     /locus_tag="PLL28_06660"
     CDS             complement(9863..11077)
                     /locus_tag="PLL28_06660"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNF69040.1"
                     /translation="MKSQEKQPWEKKKIQEKPWEKRTPQEKPWEKKTGPRKFSDDQRE
                     ERPRRERESKSFRDTFDSRETKRGGKKSFLDQVMGPDAAKKTRAKQSDNTKRNPNRKT
                     ERLQRAIHYAKQRTVGKDETREIGDERTFKKSDEQTFEKSLKRNPKKKIETTPVIKSN
                     TGTPARNDNTVRLNKFLANAGVAARRKCDEIIAEGLVKVNGVIVTEMGYRLKPDDKVT
                     YKGKAVKPVNYVYVLLNKPKDYITTVDDDRGRHTVMELVAKATTERIYPVGRLDRNTT
                     GLLLLTNDGELAQKLMHPKYGAQKLYEVELDKQLSQTDMQKIADGVELEDGVAYVDGI
                     DYAHVAKKNVIGISIHIGKNRIVRRIFEHLGYEVVRLDRTLYAGLTKRDLPRGRWRHL
                     TEREVVKLKYLK"
     gene            complement(10968..11834)
                     /gene="scpB"
                     /locus_tag="PLL28_06665"
     CDS             complement(10968..11834)
                     /gene="scpB"
                     /locus_tag="PLL28_06665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNF69041.1"
                     /translation="MNDLQKLIEALIFSSEQPISAAELQTVLFTYSGEEVSLEAIQEH
                     VEGLIGKYESDDFVFEVVKTGGGFQFLSKPYYHKPITVLLQHRSKRKLSVAALECLSI
                     IAYSQPVTKVDIEQIRGVNCDHTIQKLMERDLIRISGRAETAGRPLLYGTTQYFMDYF
                     GINTLEELPKLKEFEQKEVSIGDPSDIMEGMTDEEVYNKVQLNNERRDDAQTDNNEEN
                     QQSDDQQEVAAEAEAGAGDEPLIYEENEQETMGGDEISGETTMGEEENSGETMGKEDA
                     SGETMGEEDRAS"
     gene            11937..13103
                     /locus_tag="PLL28_06670"
     CDS             11937..13103
                     /locus_tag="PLL28_06670"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNF69042.1"
                     /translation="MQRIITFFAALIISFTALNAQGIDFQPMSLEDALISSAKSQKPV
                     FFMAYQSTCGHCEKMMKEVFPDTAVSNFYNANFICLKVDMLDQNEAKKYIRRFYISSF
                     PTFVILNAQGEVLSQYVGEFKAQEFLNQGQIALKPEQQLPPLRAAFEANPADSIACYK
                     YLLALSRGRLSTQPIANTYFRANGNTLAFTTNNWKIISMSVSDIESPIFKYTLANRDS
                     FALASSVKKVDRKLYLAAAYNMQTDISLGDTSAYFHHRDIAKTFALHTIDSLIFVNDI
                     NTYEKYKLWDRYVSTARTGAENFVWDDANMLRRIADNMLQYGNEKSAYIKGANFAVRS
                     AELKPDYFSNLSAAKLFQKAGYSEQAKKYAQAAVDEGNKKNMNVSEATMILSQN"
     gene            complement(13565..14452)
                     /gene="atpG"
                     /locus_tag="PLL28_06675"
     CDS             complement(13565..14452)
                     /gene="atpG"
                     /locus_tag="PLL28_06675"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNF69043.1"
                     /translation="MSGKLKEVRTRISSVMSTQQITKAMKMVSAAKLRRAQDAILQMR
                     PYALKMGELLSNIAEAAGDDVKIDLAQKREVKNVLLVAITSDKGLCGAFNSNVVKALK
                     RTIEERYAHLSNSNIHVMFIGKKAHDQMKRMPYQLHTEYINLFHQLSFEQVAAATDYI
                     KQGFLKGEYDEVIVAYNKFKNAATQILETEQFLPIQKSAPVAGEKKSRTDYIYDPDKE
                     QMILELVPRILNTTFFRYVLDSNASEHGARMTAMDKASDNANELLKELKISYNRARQA
                     AITTELTEIVSGAAALQGS"
     gene            complement(14463..16046)
                     /gene="atpA"
                     /locus_tag="PLL28_06680"
     CDS             complement(14463..16046)
                     /gene="atpA"
                     /locus_tag="PLL28_06680"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNF69044.1"
                     /translation="MVDVKPDEISAILRQQLSNFKTSSELEEVGTVLQVGDGIARIYG
                     LNNAKSGELVMFENGIRAIVLNLEEDNVGVVLMGASDAIKEGDTVRRTGRIASIKVGE
                     SMVGRVVNTLGEPIDGKGPLQGTMYEMPLERKAPGVIYRQPVNEPLQTGIKAIDAMIP
                     IGRGQRELIIGDRQTGKTAIAIDTIINQREFYEAGKPVYCIYVACGQKNSTVAQVAKT
                     LTDHGAMDYTIIVAASASDPAPLQFYAPFAGAAIGEFFRDSGRPALIIYDDLSKQAVS
                     YREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKIINNDSIAAQMNDLPESLKPIVKG
                     GGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLENNLFNSGVRPAINVGISVSRVG
                     GNAQIKSMKKVAGTLKLDQAQYRELEAFAKFGSDLDAATMAVLNKGKRNVEVLKQKQY
                     SPLTVEKQIAIIYLGTKGLLGDIPVEQVKAFEDAYLTYLETHHKSTLDLLKAGQFNDE
                     ITSVLEKVGIDMSKQYKVA"
     gene            complement(16087..16647)
                     /gene="atpH"
                     /locus_tag="PLL28_06685"
     CDS             complement(16087..16647)
                     /gene="atpH"
                     /locus_tag="PLL28_06685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNF69045.1"
                     /translation="MSATKLAARYAKSVLDLAKEQGKLELVLSDMRLIQSAIDSNREF
                     YLMLKSPIVHGDKKMEVVLGVFHNKVNEITESFIGILMRKNRESYLPEIIQAFIEQYN
                     TLHHITPVTVITAQPISEELHNTIINRLKTEAGLMKVELTQEVDPALIGGYVLRWDNK
                     LIDNSIARGLTLLKDEFDNNDYIRKF"
     gene            complement(16687..17196)
                     /gene="atpF"
                     /locus_tag="PLL28_06690"
     CDS             complement(16687..17196)
                     /gene="atpF"
                     /locus_tag="PLL28_06690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNF69046.1"
                     /translation="MVLLGSFDIITPDPGLFIWTVLVFLILVWILNKYAFGPIKGALK
                     KREESISEALAQAQKAREEMASMKSDNEKILNEAREERSRMLREAKEARDLLINEARE
                     KANAEYNRIVEEAKGAINNQKMAAMIEIKNSVGNMVLEVSEKVLRRELSNKNDQEQYV
                     KQLVDEIKL"
     gene            complement(17289..17441)
                     /gene="atpE"
                     /locus_tag="PLL28_06695"
     CDS             complement(17289..17441)
                     /gene="atpE"
                     /locus_tag="PLL28_06695"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNF69047.1"
                     /translation="MGVGRIGGSALESMARQPEVLGEIRTNMIIAAALVEGVALFAVV
                     VALLQG"
BASE COUNT         4927 a         3679 c         3570 g         5177 t
ORIGIN      
        1 tcattcacat attacgcaat gtaatgggtc aggaggtgat agagcttttc cataaagagc
       61 atgatctgcg tggacttacc accgccgact gcgtgatgct tcccggaggg ttttcctatg
      121 gcgatgcgct cagaaccggc gccatagcac gattttcgcc tatcatgcaa gccgttattg
      181 accattgtaa caagggcgga tatgcctggg gaatatgcaa tgggtttcag attctttgcg
      241 aagccggatt gttgccgggc gtgcttttgg agaataataa tcagcagttt atctgtaaga
      301 atgtcttttt aagtgccgat aataatgcca gcaggatcaa tgcaacgctg ccactgcaac
      361 atgcactcaa gataccaatt gcccatggcg aaggccgtta ctatgctgaa gctcgtgaac
      421 ttgaggcact ggaagccaac aatcaggtaa ttttcaggta ttgcgacgac catggtcata
      481 taaccgaaag ggctaatccg aatggctcgc tgaataatat agccggtgtt tgcaatgcgg
      541 ggcgcaatgt atttggcatg atgcctcacc cggaacgcgc tgctgaagag cgtttgggca
      601 ataccgacgg ccgcctaatc ttcgaatcat tccttgcatt aaccgaggcc acagcagcaa
      661 gataacagca cctaagccgg tttctcgtaa tatttttgtc aatgcctacc caacagaacc
      721 tgtaggggca gcttttcgtt aaatttgcat cactaaagcc gaaagatgaa gaggttactt
      781 attgcattat gctccctgtt tctgtttacg aacgttaaag cccaaattct ggaacccgtt
      841 aaatgggagg tctccagcac gcaaacagga gtaaacgagt atgtgctgat cttcaaagcg
      901 acagttcagg aaggatggaa aatttatagt cattctgttc cggaaaacgg accaatacct
      961 acaagtctta ttttcgaaaa tcttcctgca ggtgttcaac ttgttggcga aatggaggaa
     1021 ctggggcgta tggaagaaca gcaggagcca cttttcgata ataatatcat taaattctat
     1081 cacaaacgtt tgaccttgcg tcaggcagtg aaaatctcag gtaatgcaaa aattaccgga
     1141 tacatcgatt atatgtcgtg caacgatcag acatgcacag cgctttctta tgattttagc
     1201 ttcgacctgg aaggacaaag tggttttgtt caagtgccgg gatctgacta cgccaatgta
     1261 aaatatccgg taacatggaa aattggcagc gaaaaattaa acgataacga atattatatt
     1321 acctattctg caaccatagt tcctacttgg aaattgtatg cacaaacttc tccggaagga
     1381 gcagcacgac caatgaaatt catttttgaa aatgatggac agggatacac cagaattggt
     1441 ggtgttgaag agatcggtca tatggaggaa aagaaggagc aattatttga gaacaaaacc
     1501 gttcgctttt atcacgatga agtaatgatg cgtcaacgta tcagaataga ttcggcaaat
     1561 gcaaatatta ttgggtatat cgattatcaa acatgcgatg ccacacaatg taaattcatg
     1621 caaaaggatt tcaatctggc attatcggca accgggttaa tcgaaacagg ctccactact
     1681 ccgatagata cgggaggaat gaaatttggc gcctttgcag tagatctaaa gtatgctgaa
     1741 aatgcaatgt gcgaaggcga agtaaatgca cagatcacca atgcaaccgg caaaggattg
     1801 ttcagcacat ttatactcgg tttcattggt ggattaattg gccttttaat gccttgtact
     1861 ttcccgatga tcccatttac aatttcattt ttcaccaaac acgcaggcaa tcgcaggaag
     1921 ggtgtgatga atgcagcatt ttatggattg tgtatcgtgc tggtatattt cattgcctca
     1981 ctgccatttc ttttattcgg cgccggtggc gaccagttga atgaattttc gacaaacaat
     2041 attgtcaacc ttgttttctt ctttgttttt attctatttg ccttatcact ttttggttta
     2101 tttgaaataa agcttccaag caaatgggct aacaaggctg atagtgcctc agatgtcgga
     2161 ggcttactcg gtatattctt tatggcactt acactgtgca ttgtgtcatt cagctgcaca
     2221 ggtccgatct taggatcatt gcttgctaac agcattaaag atggtggtgt tgcattaaca
     2281 atgggtatgc ttggttttgg aacagcattt gcattggcat ttacattatt ggcattattc
     2341 ccacaattat taggtaatct tccgaaaagt ggcggctggt taaatgaagt gaaaatcgtg
     2401 ttcgcattta ttgaattagc aatggctgtg aaatttctaa gtaatgtgga ccttgcttat
     2461 cactggggat atgtaaaacg tgaaattttc ctcggcattt ggactttaat tgccctgttg
     2521 attgtattat atatcagcgg agtgatcaga tttccacacg atgataaaaa agttaaatgg
     2581 tcgctgggaa gagtttcatt tttgataaca tttttatttt ttaccatata ctttggacaa
     2641 ggacttcgca aatacggtga ggttggtttg ttgagtggat ttcttccacc gcaatggtat
     2701 agtatttatg aatatggcac tgaatgccct cttggattaa actgctttca tgatcttgat
     2761 gaagggatag cttttgcaaa ggagcataat aaacccattt tagtagactt caccggttat
     2821 gcatgcgtaa attgcagaaa gatggaagag aatgtttggc ctgaagaaga agtgtttaac
     2881 attatcaata atgactatgt attagtgagc ttatatgttg atgacaaaga agcattgcga
     2941 ttagaagatc aatatatatc tgccgctaca ggtcaaaagg tacgcaccat tggtaacaag
     3001 tggagtgatt ttcaaagcag tcagttcaat actaactcac agccatacta tgtattgttg
     3061 gcacctgacg gaaaaatgct gaatcagcct cgtgcatata ctccgaatgt ctcagaatat
     3121 cgtggatttt tagagtgtgg tattgagaca ttcaagaaca tggataaaat tgctgcgaag
     3181 taattaagcc cttagcatca cgggcctcta tactcaaaat caaattaata tttttttatt
     3241 gcgaattgtc gttcagataa ttcgcaattt ttttttcgcg cacgctaaat agagaaatca
     3301 tataacattg gggttatcta tgtatgagaa tgatatctcc tataaagtga ggggtgcgat
     3361 attcacggta tttgacgcct aggggcaggg gttgccggaa tctgcatatg aggcagcatt
     3421 atctcacaaa ctgatcatag ccggattgaa tgttagacgt caggtttcat tgccattggt
     3481 atatgatggt gtcaaaatgg atatcggata tcgtatcgat ctgcttattg aagatcttgt
     3541 tattatagag ataaagtctg cagagaatct cactaaggtc catcacaaac aattacttac
     3601 atatctcaag ttatccggcc acaagcttgg catactagtg aatttcaatt gcagcgatat
     3661 caccaaaaac atttttcgaa aggtcaacta tttataaacc atattgagcg aaccattgct
     3721 tgtcaattaa tcaaaactac atccaactaa cattcgaaca cttttctgtg ccaatctgcg
     3781 agatctgcag gacattttca atatctccat ccaaactaat cgctttaaac atagtatgaa
     3841 atgaataaat aagtattgtg aagtttatgg tgcgtgtttg tttaccgctg attacgcaga
     3901 ttttcgcaga gtggtgtggt gaagattttg gttgaaataa aaaaaggaga ctgtgatgca
     3961 gtctcctttt ttttactgat gtaatgatca attaaatgtt ggtttttcag gcagcgtata
     4021 tgttctgccg gcgttttgtt ttttcaggta atccatcaac ggcatgaaat aatccaacat
     4081 tggttttgca ctcatgtcgg agcctataga agccttgagg tgttcgcgcc aatcaacggt
     4141 tgcaccaggc gtcattacct tgcgaaggaa atcgcctgtt tctttcgaac cccaataatt
     4201 ggtagcatgc ggatcttgat gcaaaatatt ttttgcgata taatcatgga actggaacaa
     4261 caatacattg ctgatactgt aatcataata ttgagcaggg tcatcattga tatgtgtttt
     4321 tgttgcagca tcacagtagt gctcatcgcg cggtgacggc ggaacaatac cttgatattg
     4381 cttcactaat tcccaccatt ttgcgttgta ttgatcagga gaaaggtttt tcgcatataa
     4441 tgcatattcg aattctgtca taacaccact tccccaaggg atatgaacaa tatagtcaag
     4501 tgcttcactt aataatttca atgtgtcatt tgtaacaaca cccggtttga tcaaaccttg
     4561 attttcaagc aagggttttt gcaaactggc gagaccaatc atagttccaa acgcttcatg
     4621 atatccgcga ttggcacctg tgcgcaaaac cggaggaaca tcaggattag aatagctcat
     4681 ataatagtag atatgtccaa gctcatgcaa tacggtactc caccattcgg tattagcttc
     4741 cacgctcata agtgaacgca catccttgtc gagatcgata tgccaggcgc tggcgtgatt
     4801 attttttgcg tatccggcat cagcactcac aggatataat gatgattttt cccaaaatga
     4861 ttgcggcaat gcgtcaaatc caaggctcat ccaaaatgct tcaccttgtt tcatcaccca
     4921 ttcggcatcg tgctttttta attccggatc tatatctaat ccttctacat taaccattgc
     4981 agtccagtct tgtccccagc ggtttggcaa ccattgtgcc gggatataat caggaacagg
     5041 ttgattatat ttttctgcca gctcatagcg tgcccaagta tgcaattcgc gatatagcgg
     5101 ccaaacatct gtgataaagc tgcgtgtaag ttcaagcatc tcttcgctgg tcatattata
     5161 ttcactggcg agataagcga agaaatcgga ataaccgaga ggtgttacac tttcgttgcg
     5221 caatttttgt aattcaacaa gaccatcttt taggcctacg ccaacttctt tactggctgt
     5281 ccatgcggca agacgttcat tcatattatt tgaactgcgc aaaatctcat ccagttgatt
     5341 ggtagttaca ggcttaccat taagggtata tttaaagcca aatagttttt ctacctgagc
     5401 atttgttgct gtgattcgat ttttcacaac atcgcccgct gtttccggat tattacctgc
     5461 caggtagcgg atatattcca attgacgaac ctgaatagct gtaagcttat ctttcatcgc
     5521 caaatatttc tgcgctgttt caatatttgt tttgcttcca gtgaatttgg cgtatgcttc
     5581 atcggcgatt gcagcattgt gacttgccat tgtatcgcct tcaacgatat gtgtctgtaa
     5641 tatccactgt ccttcagaag aagcggttac aagtttttga tactcggcgt tatatccgtc
     5701 gagataagtt tgcacctctt tctgaattgc gctgttatct tctgcctttt cttttggttt
     5761 gcaggaaatc aatgctattg gaatgaggag actcagggtg agtagttttc tcatattggt
     5821 tggtatttta ctgctaaggt aatgaagcta ttgtggcttt tacaggctct taacacacct
     5881 taattatctc gttttttttg cacaatatgt tcacaaccta aagatttttt ctacggaagg
     5941 gcaggaaatc tttagcttcg catacgcttg cgcaatgtgc gtagcaattc acttaatcct
     6001 taaaaacaag aacaaaatgg cagaagcaac caacgacaaa gtaaaagccc tagggcttgc
     6061 agtagacagt ttgaataagc tgtatggaaa aggcacggta atgaaattag gcgagtcagc
     6121 cgtaacagca gaagaggtta ttcctacggg atctcttgga gtggacatgg cattgggcat
     6181 aggcggtatt cctaaaggcc gtgtagtaga aatctatggc cctgaatctt cgggtaaaac
     6241 aacattggcg attcatgtga tcgctgaggc acagaaaaaa ggtggaattt gcgcgatcat
     6301 cgatgccgag catgcctttg ataaagtgta tgccgaaaaa ttgggtgtgg atgtagagaa
     6361 cctgcttatc tcacaacctg attacggtga acaagcactc gagattgctg atcatttgat
     6421 ccgctccggc gcattagatg tagttgtaat cgactcagta gctgcattgg ttccaaaagg
     6481 cgaattggaa ggcgagatgg gcgacagcaa aatgggactt caggcacgac tgatgagtca
     6541 ggctttacgt aaactcaccg gtacgatcaa caaaaccggc tgttgctgta ttttcatcaa
     6601 ccagctgcgc gaaaagatcg gtgtgatgtt cggtaatcct gaaactacaa caggtggtaa
     6661 tgcattgaaa ttttatgcat cagtacgtat cgatattcgt cgcattggtc agttgaaaga
     6721 caaagacgat gtgatcggta accgtgtgaa agtgaagatc gtaaaaaaca aagtagctcc
     6781 acctttccgt caggctgaat tcgatattat ttatggtgaa ggtatcagca agatcggtga
     6841 gatcattgac cttggtgcag aaagcggtgt gattcaaaaa tcaggcagct ggtatagcta
     6901 tgaaggcacc aagctcggac aaggacgcga aggtgttaaa gatatccttc gcgataatcc
     6961 cgaactcgcc ctcgagatcg agaaaaagat caaagcaaaa ttgtcgggtg tgccgactaa
     7021 ggaagattaa ctacacaaaa gaggcggttg caaacaaccg cctctttttt ttggggcctc
     7081 gccccaaaag tcgcagggtt cgcccgcgga aaaggatttt cattggataa aaaatttnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnagg gttgcctttt tcttacgaag
     7261 agagattttc ttgcatgtaa gatttttttc cgaagcggaa tttttatgcg cgaaccctgt
     7321 tgggtgtgtg ttgcggggga ggggggtatg gtggttgctt cgcaaccacg aaaaattgtg
     7381 agatttttct aagcgcttaa agcgctcgtg ataatatgca tagccgcgcg aagcgcgtat
     7441 aagcatgtat gttttatgaa atggtgtgaa ttcattatag gtgtatgtat ttttatttca
     7501 tgactacatt taaaactcgt gatgaacagg atgtcatttg gtttatcaca tttacttgct
     7561 acaggtggtt accattgatt gaaacgaccg atttatatga tgatatctac aaatggttcg
     7621 atatattgaa aagcgaaggg cattccattt tgggatatgt catcatgccc aatcacatgc
     7681 acatgctcat gtatttttct aaaaataaaa gaacaataaa tacatgcatg gctgaaggta
     7741 aaaaattcag ggcctgggaa attattaaaa gattgaaaaa acaaagggca tcacattatt
     7801 taattcagat ggaatgggtg cctgaagacc ataaaaaccc taacaaacag aagttcatgg
     7861 cctttgaacc ttcctttgat tgcaaggttt gtaaaagcga agaacgcgtg ctgacaaaac
     7921 ttaactatat ccataaaaat ccggtaaaca aaggttatca cctggcccct tcaatgactg
     7981 catacaaaca ttcaagtgca aaatactatc acacaggcat ccactctgcc tatcctgtcg
     8041 aacattataa gcgatttatc aggccattta actgcaatat agatgcccct caataaaaat
     8101 tataaaattt taattcaatc actattggtt cgttcatctg aaacttcaaa aaactcagat
     8161 attttgaatt tttgtcatat taaattccca tgttatgaat atcaaagcta attgctacac
     8221 tctcctgata gccttactta cgagaattcc tgcatttggt caactcgaag cagtagacac
     8281 aataagctac ccggttgatt ctgagcattc gacctatgtc tttcaaaatc tagggaatga
     8341 cggatacatt ctgatgtaca aaagcaacag cgatagggta gaattatcga agtatgatac
     8401 tctgaataac gaaacaaatc acaattcctt tgaggttgtc gcacatctaa gatatgctgt
     8461 atatccagat aaatcctata gtgattactt tgatatctct gaaaacaacg aggttgtcaa
     8521 tcatagaatt gacttcaaga cactagatgt tgtgacaaca aagtgttctg ttgacataaa
     8581 actcaaatac aaataccagg tttccaataa cgactgtata ctccttgtat ttgaaaatta
     8641 tttcaagcag gaaatatata aagtagatat caaaacaggt caggtcaata gaataatatc
     8701 tgatttgccg gaaaaaaaga aaaagtcatt tgcctatcaa caaattacag ttgtaccatc
     8761 gaccggtgaa ttctttttat ttaccaacaa acaaaaattc ttcaagacat acaacctctc
     8821 cgcacacctt aataatgaag gtgagcaaat aggagcttac gtgttgaacc ccaaaccgtc
     8881 gaaattttat caaaattaca cagtggtcgc aacgcctgat aatgaatacc aaatttggtg
     8941 tgaaatgaag tatattaaaa aattatccga ttatggtaat cccaaaattt cgggcttatg
     9001 tcaaattgta attgatgacg aatcagttgt aagccggaaa tcaattccat ttcgctcctt
     9061 caagaacttt tatgcatttc aaaaggataa taaatcgaaa aacatagata aaaactccct
     9121 taaatatcgc atgggccacc ctaccaaggt aaatatgcat acaattcaaa taaaacttgt
     9181 tgatgatgct gtatactacg ccggagaagt atactggccg gtatataaaa cagttgttga
     9241 gcaaaccgtg ggttcaacag ccaaacctac caaaaaaatg gtattagatt atgttattta
     9301 ctctcatgca gttatcgcaa aatttgacct agaaggtaat atgatctgga ataattcagt
     9361 agaattggac gtagatgata ctgaagccgt tttgcaaatc aataagtatc aaataaataa
     9421 aaatggagtg ggagtccttc tgatcaatga aggagcgatc acattgacaa aatttgacgc
     9481 tgacggtaga attgtctcca aatacaacac cccatgtagc gcacctattt gcgttgacga
     9541 agaaaaaagg aaatttaatt tctttagtct ccaatattgg tacaatgatt acatggtgta
     9601 ccctgagtat acgatcgatg atgacccctc tacagagcga accgaaggct acttaaattt
     9661 gtacaaatac gaattgatac aggattaagt ccgcagacag gcatcaatca agttcccata
     9721 aaacaagaaa ggtcgcgccc tatcggggtt cgaaaccatc tacttaaaat tcatgatgta
     9781 tcatcgaatg atcaattaaa tctataagca cgaagaatca attgatctta agataagccg
     9841 caattggtaa atttacaatc tatcacttca aatacttcaa cttaacaacc tcccgctcag
     9901 taagatgcct ccaccttcct cgcggcagat cgcgcttagt gagaccggca taaagcgtgc
     9961 gatcgagtcg aacaacttca taacccaagt gctcgaatat tcgtcgcacg attctatttt
    10021 tcccgatgtg gattgaaata ccgattacat ttttcttagc cacatgagcg tagtcaatac
    10081 catcaacata agcaacaccg tcttccaact cgacaccatc tgctattttt tgcatatccg
    10141 tttgtgacaa ctgcttatct aattcaactt cataaagttt ttgcgcacca tatttcggat
    10201 gcattaactt ttgtgcaagt tcaccgtcgt tggtgagtaa caataaccct gttgtatttc
    10261 tatcgagtcg accaaccgga tatatgcgtt cagtagtggc tttcgcaaca agctccatca
    10321 ctgtgtgtct gccgcgatca tcatccaccg ttgtaatgta atcttttggc ttattcagga
    10381 gcacataaac ataatttaca ggctttaccg cttttccttt gtaagtaacc ttatcatcag
    10441 gtttcaatcg ataacccatt tcagtaacta taacaccatt tactttcact aatccttctg
    10501 caattatttc atcacatttc cttcgcgcag caacaccggc atttgcaaga aatttattta
    10561 aacggacagt attatcgttg cgggcaggag ttcctgtatt tgatttaatt acaggtgtag
    10621 tctctatttt ctttttggga ttgcgcttta aacttttttc aaaagtttgt tcatcacttt
    10681 ttttgaatgt gcgctcatcg cctatttctc gcgtttcgtc ttttcctact gtgcgttgct
    10741 tggcataatg tattgcacgt tgcaaacgtt cagtcttacg attcggattg cgcttagtat
    10801 tatcactctg tttcgcgcgg gtcttttttg cagcatcagg acccattacc tgatccagga
    10861 atgatttttt ccctccgcgt tttgtttcac gggagtcaaa tgtatctctg aaagattttg
    10921 attcacgctc tctgcgtgga cgttcttcgc gttggtcgtc agaaaattta cgaggccctg
    10981 tcttcttctc ccatggtttc tcctgaggcg tcctcttttc ccatggtttc tcctgaattt
    11041 tcttcttctc ccatggttgt ttctcctgag atttcatctc ctcccatggt ttcctgctcg
    11101 ttttcttcgt agataagcgg ctcatcccct gctccggctt ctgcttctgc ggcgacctct
    11161 tgttggtcat ccgattgttg gttctcttca ttgttatcgg tttgggcgtc atcacgacgc
    11221 tcgttgttta attgaacctt gttgtacacc tcctcatcgg tcatgccttc catgatatcc
    11281 gacggatcgc cgatgctcac ttccttctgt tcgaactctt tcaatttcgg caattcctcg
    11341 agggtattga taccgaaata atccatgaaa tactgcgtag tgccgtatag caatggtctt
    11401 ccagcagttt ctgccctgcc cgagatgcgg atgaggtcgc gctccatcag cttctggatg
    11461 gtatgatcgc agttcacacc gcgaatctgc tctatgtcga ccttggttac aggctgacta
    11521 taagctatga tagacaggca ttccagcgct gctacagaca atttccgctt agagcggtgc
    11581 tgtaaaagga cagtgatggg tttgtgataa tatggtttcg acaggaactg aaagcccccg
    11641 ccggttttca ctacttcgaa cacaaaatcg tcgctctcgt acttaccgat caggccttca
    11701 acatgctcct ggatagcctc caggctcacc tcttcgccgg aataggtaaa taaaacggtt
    11761 tgcaactctg ctgctgagat aggttgctcg ctgctgaata tcagtgcctc tatgagtttc
    11821 tgcaagtcgt tcatcgggtt gcaaatgtag taaattggga gggtacgacc gcagaatttg
    11881 gaaggatttt tgctgtaatc tcatcctatt acctcacaag tgataccttt gaatctatgc
    11941 agcgcattat taccttcttc gcagccctta taataagctt taccgctctc aatgcccagg
    12001 gaatcgactt tcagcccatg agccttgaag acgccctcat ctcctctgca aaatcgcaga
    12061 aacccgtttt ctttatggcg taccaaagca cctgtggcca ctgcgaaaag atgatgaaag
    12121 aggtgtttcc cgataccgct gtcagcaact tctacaatgc caattttatt tgcctcaaag
    12181 tggatatgct cgatcaaaat gaagcaaaaa aatatatccg aagattttat atcagctcct
    12241 tccccacttt tgttattctg aatgctcagg gtgaagtatt gtcgcagtat gtcggcgaat
    12301 ttaaagcaca ggaattcctt aatcagggac aaatagcttt aaaaccagag caacaacttc
    12361 cacctttgcg tgcagcattt gaagccaatc ctgctgattc aatagcctgt tacaaatatc
    12421 tgctcgcatt atcccgcgga cgattgtcga cacaacccat cgcaaacaca tatttcagag
    12481 cgaatggcaa tacacttgca tttactacca ataattggaa aatcatcagc atgagcgtga
    12541 gcgatataga atcgccgata tttaaatata cacttgccaa tcgcgatagt ttcgcacttg
    12601 cttcaagtgt aaaaaaggtc gacagaaaat tatatctcgc agcagcatat aacatgcaaa
    12661 cagatattag tcttggcgac acttctgcct attttcatca tcgcgatatc gctaaaacat
    12721 ttgcattgca cacaattgat tcactgattt ttgtgaatga tattaatact tacgagaaat
    12781 ataaattgtg ggacagatat gtaagcaccg cccgtacagg agcagaaaat tttgtttggg
    12841 atgatgccaa tatgctcagg cgcattgcag ataacatgtt gcaatatggc aatgaaaaat
    12901 ccgcgtacat taaaggagca aattttgctg tgcgcagcgc ggaattaaaa cctgattatt
    12961 tctccaatct ttcagcagct aaactattcc aaaaagccgg ctactctgaa caggcaaaaa
    13021 aatatgcgca ggctgcagtt gacgaaggaa acaaaaagaa tatgaatgtt tctgaagcga
    13081 cgatgatact gagccaaaat taattagcaa attagctgat tagcaaatta gccgattagc
    13141 caattagcca attagccaat tagccgatta gcaaattagc cgattagccg atgtggtgtg
    13201 cttgcctcga tgtggtgata ttgggtgaat gcttcgggag tttgagtgtg tgggatgggg
    13261 tttttagtcc gccctgaccc cggccctctc ccagagggag agagggtagt atttgctaat
    13321 aaaataatta tagttcaatg ttgagtgact agctataatc tttaaaaaaa taacctccct
    13381 ctccctttgg gagagggtcg gggtgaggac atagacaaat ccgcatgcac caagataacc
    13441 tcaccacccg cctaacggcg aggcaagctc accactcacc acccgcctac cggcgaggca
    13501 ggctcaccaa aaaaaagacc catcaaaacg atgagtcttc aaaaaaaata tatcttcaat
    13561 gaaatcaact tccctgcaaa gcagcagcac cggacacgat ctcggtgagc tcggtggtga
    13621 tggcagcctg acgtgcgcgg ttatagctga tcttgagttc ttttaataac tcattcgcat
    13681 tatcactcgc tttgtccata gccgtcatac gtgcaccgtg ttctgatgca ttgctgtcga
    13741 gcacataacg gaagaaggta gtatttaata ttctaggcac taattcaaga atcatttgct
    13801 ccttgtcagg atcatagata taatcggtgc gcgatttttt ctcgcctgct acaggtgcgg
    13861 atttttgaat agggagaaac tgttctgtct ccaatatctg cgttgctgca tttttgaatt
    13921 tattgtaagc aacaatcact tcgtcatatt cacccttcaa aaatccttgc ttaatataat
    13981 cggttgcagc agctacttgt tcgaacgata attgatgaaa cagattgata tattcagtat
    14041 gcaattgata aggcatacgc ttcatctgat catgtgcttt tttaccgatg aacatcacat
    14101 gaatattgct attcgacaaa tgcgcatagc gttcttcaat agtgcgcttt aatgccttca
    14161 ctacgtttga attaaaggca ccgcacaatc ccttgtcgga agtaatagct accaacaaca
    14221 catttttcac ttcgcgtttt tgtgcgagat cgatttttac atcgtcgcct gcagcctccg
    14281 ctatgttact cagcaattca cccattttca gagcgtaagg gcgcatctgt aaaatggcat
    14341 cctgtgcgcg acgcaatttc gccgcagata ccatcttcat cgccttggtg atttgctgtg
    14401 tgctcatcac cgaggaaatg cgcgtgcgta cttcttttaa tttaccggac atgtatgttt
    14461 aattacgcaa ctttatactg tttactcata tcgataccaa ctttctcgag caccgaagtg
    14521 atctcgtcgt tgaactgacc tgccttcaaa agatcgaggg ttgatttatg atgtgtttcc
    14581 aaataagtga gatatgcatc ttcaaatgct ttcacttgtt caacaggaat atcaccaagc
    14641 aaaccttttg ttcccagata aataatagca atttgtttct caacggtaag tggtgaatat
    14701 tgtttttgct tcaacacttc cacgttgcgt ttacctttat tcaacacggc cattgttgca
    14761 gcatcgaggt cggaaccgaa tttagcaaac gcttccaatt cgcgatactg tgcctgatcg
    14821 agtttcaggg tacccgctac ttttttcatc gatttgatct gtgcgttacc acccacgcgg
    14881 cttacagaga tacccacgtt gatcgcagga cgaacacctg agttgaacag gttattttcc
    14941 aagaatatct gaccatcagt aatcgagatt acgttggtag gaatatacgc agaaacgtca
    15001 cccgcttgtg tttcgatgat aggcaatgca gtaagtgaac caccaccttt tacgataggc
    15061 ttcaatgatt caggaagatc gttcatctgt gcagcaatgc tatcgttgtt gatgatcttc
    15121 gcagcacgct ctaataaacg tgagtgcaag tagaatacat ctcccggata agcttcacgt
    15181 cccggtgggc gacgaagcaa cagtgacacc tcgcgataag aaaccgcttg tttagaaaga
    15241 tcatcgtaaa tgatcaatgc cggacgtcct gaatcgcgga agaattcgcc gatagcagca
    15301 cctgcaaatg gtgcgtagaa ttgcaatgga gccggatcag atgcagaagc tgcaacaatg
    15361 atcgtgtaat ccatcgcacc gtgatcggtc aatgtttttg caacctgcgc aacggtagag
    15421 tttttctgtc cgcaagctac atatatacaa tataccggtt taccggcttc ataaaattcg
    15481 cgttgattaa tgatggtatc gatagcgata gccgttttac ctgtctgacg gtcgccgatg
    15541 atcaactcgc gctgaccacg accgattgga atcatcgcgt cgatcgcttt gatacctgtt
    15601 tgcagtggtt cgttaaccgg ctgacgataa attaccccag gagctttgcg ctcgagtggc
    15661 atttcataca tggtgccttg cagtggtcct ttgccatcga taggctcgcc gagtgtattt
    15721 accacgcgac cgaccatact ttcgccaact ttgatagatg cgatacggcc cgtacgacga
    15781 acggtatcac cttctttaat cgcatcggat gcacccatca ataccacacc cacgttgtct
    15841 tcttcgaggt taagtacgat ggcacggatg ccgttttcga acatcacgag ctcgcccgat
    15901 ttggcgttgt tcaatccata aatgcgggca ataccatcgc ccacctgcaa tacggttccc
    15961 acttcttcca actctgagct ggttttgaag ttgcttaatt gctggcgaag tatcgcgctt
    16021 atttcatcag gttttacgtc taccatctga ctgaattata tattgtgaat gaatactttt
    16081 aattaattaa aacttacgga tataatcgtt gttatcaaat tcatctttca acagcgttaa
    16141 tccgcgtgct attgaattat ctatcaattt attatcccaa cgcaatacgt aaccacctat
    16201 taatgcagga tctacttctt gcgttaattc taccttcatc aaaccagcct ctgttttcaa
    16261 acgattgatg atagtgttgt gtaactcttc agaaattggc tgcgcagtga tcactgtaac
    16321 gggtgtgata tggtgcagcg tattatattg ctctataaaa gcctgaatga tctccggtaa
    16381 atatgattcg cggtttttac gcatcaaaat accaatgaag ctttctgtga tttcattcac
    16441 cttattgtga aagacaccca gtaccacttc catcttttta tcgccatgca cgattgggct
    16501 tttcagcatc agataaaatt ctctgttgct gtcgatagcc gattgtatca gtcgcatatc
    16561 cgacaatacc aattccagct tgccttgttc tttagcaaga tcgagaactg atttggcgta
    16621 acgcgctgct aatttggttg ctgacatctg ttataattta cccgtcgatt aatttgaaac
    16681 ggatgatcac aattttattt catctaccag ttgcttcaca tattgctcct gatcgttttt
    16741 attcgacaac tcgcggcgca atactttttc gctcacttcc aacaccatat tacctacgct
    16801 gtttttgatt tctatcatag cagccatttt ctggttgttg atggcaccct tcgcttcttc
    16861 aacaatacga ttgtattcgg catttgcttt ttcacgtgct tcgttgatga gcaggtcgcg
    16921 tgcttctttt gcttcgcgca acattctgct gcgttcttca cgtgcttcgt tcagaatttt
    16981 ttcattatcc gacttcatgc ttgccatttc ttcgcgcgct ttttgtgctt gagcaagtgc
    17041 ttctgaaatg ctttcttcac gttttttcaa agcgcctttg ataggaccaa aagcatattt
    17101 gttcagtatc cacaccaaaa tgaggaacac caataccgtc cagataaata accccgggtc
    17161 cggagtaata atgtcgaatg aaccgagcaa taccatgttt attcttttta ttgtttacgg
    17221 caataccgtc ttaatgtcaa tgaatgaaga tcagcagcaa ccaaccgttg ccactgatct
    17281 tgtacttttt acccttgcag aagggcaaca accactgcga agagggcaac accttccacg
    17341 agagcggctg caatgatcat gttggttctg atctcgccga gaacttcggg ttgacgagcc
    17401 attgattcga gcgcgctacc accgatacgg cctacaccga tacctgcacc gat
//
LOCUS       DAOQHY010000061        16985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1572_length_16985_cov_5.623331, whole genome shotgun sequence.
ACCESSION   DAOQHY010000061 DAOQHY010000000
VERSION     DAOQHY010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16985
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1572_length_16985_cov_5.623331"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="PLL28_06700"
     CDS             <1..1065
                     /locus_tag="PLL28_06700"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HNF69048.1"
                     /translation="DIAKYDPNAIAQPADLVAVAKKLAANPNIANKRWITTQYDSMVG
                     VRNTSTNLKSDASLIRIIGKNKGIAMTTDCNARYVYSDPYVGTMIAVSEAARNIVCSG
                     AKPAAVTNCLNFGNPYNPEVYWQFSNAIRGMGDACRQFDTPVTGGNVSFYNQSEEGPV
                     YPTPTIGMVGVLEDFKYQMTMDFINAGNVILLVGNATDDMACSEYLKDIHGITQSPAP
                     RFDLKEEARLQDTIYNLIQKRLIDSAHDVSEGGLFTTLLESAMVNQLGFEIETDHGIR
                     SDAYLFGEGQSRVVVSTTSDKVFEIEAICKSNNIPVNVLGTVTQGSIIIDGVRYGGIS
                     EYREIYDNAIGRYMQQEVTV"
     gene            1165..2118
                     /locus_tag="PLL28_06705"
     CDS             1165..2118
                     /locus_tag="PLL28_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69049.1"
                     /translation="MKNLWVYLGLISLSALGFSSCQQSANGSTNEPSGMQKSVEAFSP
                     GTEWNNYWYSGKAELTSYTLQQSRYGEIHAGTAVNIFVTEDFSKSKHVKLDDPSKAGA
                     DKLPVLKMNQSIKFNTGIYPYSIMLSSFQPVDVNQFPHAVKISGTVQEWCGMAYYQLD
                     QRDEKYQVEVRSYFESEGDKELSLPATVQEDALWNMIRIAPANLPTGEQMILPGATYL
                     RLSHKPVEAVKSQLTLQIIDGVQVYTIKMPSLNRELKIRFESAFPYRILGWEDTYPGF
                     DGVSLTTTAVRNKEMIIDYWRTHNNADRVLREQLGLPKDTQ"
     gene            complement(2128..2604)
                     /locus_tag="PLL28_06710"
     CDS             complement(2128..2604)
                     /locus_tag="PLL28_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69050.1"
                     /translation="MRSLVLFGLVLALCTSCNKRKYAAATEYNDKVIRFVEQSDRAMK
                     VWNSNNFMQEYMIKKHNTVMRLLNMQDSLANTEPLTGDDTLRQAAIIMVENYIESFVV
                     FDTIYAILSDSTYYPEDSIRIHELLSSNRNMLQQQGNNFVETQKRFAIRHDLPFVE"
     gene            2639..3637
                     /gene="rfaD"
                     /locus_tag="PLL28_06715"
     CDS             2639..3637
                     /gene="rfaD"
                     /locus_tag="PLL28_06715"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HNF69051.1"
                     /translation="MQAGSDNHLKTVVVTGAMGFIGSCLAAALHRTGAWRVVISDDFN
                     RPDKMQNLIALQDVLKVERDMIAAWVDSQPAGTISWILHIGARTDTTEFDHAIFDRLN
                     RNATKALWEVCCKQQIPLIYASSAATYGSGVHGYVDNHALIPELQPLNPYGWSKQDID
                     MWVLKQQTHPPFWYGLKFFNVYGPNEYHKGRMASVIFHAYNQMQATGTIKLFRSHNDA
                     YRDGYQLRDFVYVLDVVDVIQWLMHNKPASGIYNLGTGKAEPFYQLAASTAEALNIPL
                     QIDWIDIPEDIRDKYQYFTEADMSKLRKAGYTRAFSSLEAGVADYVKNYLVRGNYY"
     gene            complement(3641..6037)
                     /locus_tag="PLL28_06720"
     CDS             complement(3641..6037)
                     /locus_tag="PLL28_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF69052.1"
                     /translation="MFNEIMRSITFLLLISISIHAFPQNGKIAGTITNSINNEPVPFA
                     TVVLAGTSYGAVSDDLGNYSIEGIEPGLYNIECTFIGFKKVAIPEVQVTNAKPVFINI
                     QMQEDLNVLDAIEVKAPVSDKTEESPLSLRTIGANEIQRNPGGGRDISKVIQTLPGVT
                     YTPSFRNDIIIRGGAPNENRFYLDDVEVPNINHFATQGSSGGPVGLINVDFIREVEFY
                     AGAFPVNYGNALSSVMSFQQRDGRSDKWGGTFTVGSSDLGITMEGPIGEKSTVLLSAR
                     RSYLQFLFGAIGLPFLPTYNDFQYKQKIKLDKQNEITIIGLGAIDQFDLNLEDDTSAF
                     QQYVLGNLPVQTQWNYTLGVVYKHYREKGYSAVVASRNMLNNEAYKYENNDDSNPENL
                     IYNYGSQESENKFRYEETANIGRFRIKGGLNYELARYTNSTYQLLPLGTDITTIDYTS
                     KLFVNKYGAFGQASITLLDYRLTLSLGLRLDGNDYSSSMANPLQQLSPRLSASYGLTD
                     KWSLNFNSGIYYQMPAYTVLGYAEGENNFVNKENDLRYIQSSHLVAGLEYRPESYLRF
                     TLEGFYKNYARYPFSLRDSISIANQGADFGVVGDEPVTSTSNGRSYGVEFLAQQRLNK
                     NYYYIISLTYVVSEFENGDNVYTASSWDNRTIISMTAGKKFKKNWELGLKWRYSGGLP
                     YTPYDQEYSSLTYVWDIYRQGVPDFSKLNAERLDPYHQLDMRVDKKWFFDKWNLDVYV
                     DVQNVYNFQTTFQPYIDVVKDADGNPVINPDDPTRYLTYDLENETGTVLPSIGIIVEW
                     "
     gene            complement(6321..7451)
                     /locus_tag="PLL28_06725"
     CDS             complement(6321..7451)
                     /locus_tag="PLL28_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNF69053.1"
                     /translation="MKQKWTIHSLIVGMSILAVSCSKLNSADSGPQQVSETNTTDTEV
                     VTVDTLHQPADGPPGMVWIPGGTYMMGTDTDPQRRSDETPAHPVKVDGFWMDITEVTN
                     AQFAEFVKATGYLTTAERPIDWEELKKQVPPGTPKPADDLLQPASMVFTSTDHPVDLN
                     NFLNWWKFVPGASWRKPQGDTSTIKGKNNHPVVQVSWDDAVAYCKWAGKRLPTEAEWE
                     FAARGGTTDQIYAWGNDKQLAKHTNSWTGDFPYNNTHEDGYVTTAPVKSYEPNGYGLY
                     DVAGNVWEWVSDYYHSEYYAACKLEGTLVNPQGPKHWYDPNQPFNEVHTKRGGSFLCN
                     EVYCSSYRLTARMATSYDTGQDHSGFRCVMTQEQWEERRNEK"
     gene            complement(7505..10996)
                     /locus_tag="PLL28_06730"
     CDS             complement(7505..10996)
                     /locus_tag="PLL28_06730"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69054.1"
                     /translation="MYNLRYLTLSLLIIPMFLYGQLDQPKPALTADAPQWVQLMYTET
                     PNAYAIQDAFDTYYKTHEFEENNYTRYFQRWAILARPYVLENGDVHFPTHMQLMREIN
                     SRTRSQVIDEGSRSTTWSFAGPDIHFTTKYDAGATQIPVSDHMNTYSFDRSKSNPNIA
                     YCGGETGGIYKTTDQAMTWTHMTHGYYINTVRALAVDPFNPDIVIASSEGELWRTTDG
                     GITWNIMGDAAFQAIYITAYDIVFNPENSNIVYAGCEQGFFRSTDGGSNWTEILDHFC
                     MSVEVKPDNAAIIYTLQYDPTTQIPYFKKSFDYGATFTTYSDGWFTVPAADMGKIESL
                     GGRIGVTEANPDKVYVLLVGESDADAGLQLRGTIGVYMSNDSGESWSFPHGLIGMPYN
                     VDTHPNLMDFDGHSSDYDQIYYNTTIAVSQLDENKLLIGGLNLWKSEDGAVSYDPVGG
                     YLGYLPYYHPDNQETKIYKTSPTTEEIWMATDGGVNYTTNWVESHESRSNGLRGSNFW
                     GFDQGWQEDMMVGGRYHNGNGAYHENYPEGEFLALGGGEAASGYVKQSDENICMFSDI
                     GGIKLPDEIEGIAEHLAFSNAPNESYWYNSSSRIQFDWDYFDHAYMGRDNIIWKSTDG
                     GGGFTPLHEFGTNANNKVLWVEQSRVNTNIIYCTQNIGSTNCYLWKSTDRGLTWTNLT
                     LPQAKRDLIFTLSGDNADELWIAYKYGPNGSKVYHSINGGDSWVNITTATLNDMHPSA
                     IVHQFGTDGGVYVFMREAKVYYRTNTFIDWLQHGNDLPLVIDPLKAIPFYRDDKIRLG
                     SWNIGIWEAPLYEPSTLIADFSAEKPNFYCAGDQIKYVDHSTAGAGATYAWEFPGGIP
                     ATSSEQNPVVTYPTEGVFDATLTITDGAEVQSITKSAIVSSLAPSTLPLSENFETAQF
                     PEGWHGGTSGGWGIAEDASAYDVGTYSMFFNNYWTDLQGDHVDEITAQYDIDAAITAQ
                     LTFDVAYRPYGGIYMDTLAVYVSNDCGATLDLIYYKGGNTLATGPAYTADVFYPAADE
                     WRTETIDLTPYIDDVSLMLVFRNIGYWGQAMYADNINIAVEKEGITSHEENISVQIYP
                     NPNSGKCYAIINCDEAVNAELQLLNTVGQVVYQSAANLIPGSNTIYIDARDLPAGVYT
                     MSVKTSTLNISKSIIIE"
     gene            complement(11011..11727)
                     /locus_tag="PLL28_06735"
     CDS             complement(11011..11727)
                     /locus_tag="PLL28_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HNF69055.1"
                     /translation="MTTKPVFLTAKWQNLLMANYVVDPEILDPWLPYGTAHDTFDGHC
                     FLSLVAFEFNNTRVKKIAFPWHKNFVEINLRMYVKRIENNQLKRGVVFIKEIVPRHLI
                     SFFANTLYNENYVTMPTGGGLFRTNEHDFVGYTWGKKYSMEAKLAPEKKLLIPGDISE
                     FITEHYWGYASINNNSTYEYAVEHPRWETRDIIKHTIQVDFATLYGDTFSFLNVATPH
                     SVLLACGSDILVRHPNKIKP"
     gene            complement(11810..12661)
                     /locus_tag="PLL28_06740"
     CDS             complement(11810..12661)
                     /locus_tag="PLL28_06740"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HNF69056.1"
                     /translation="MKKILTIGLLLITAATQKLSAQELELPQPSPWAQVSQKFGLAQA
                     TVTYSRPSMKGRTIFGSLVPYGEMWRTGANKATELKLEGNVAINGQILPAGAYSLFTI
                     PGEKEWTVIINKNTELWGTGGYKQEEDAMRFQVKVQSHTSTESFTIGFSNIKDNSAVL
                     EIYWETTMISFELTNDFLEQGKTNITEAIAAAENTMSLYNDAAEFYLDNNLDAKQALE
                     WAKKSVAQRERYWNLFTLAKAYAKNGMNKEAIETAEKALTLSNEAKNTGFSQGIEKEL
                     NKWKSNK"
     gene            12734..13693
                     /locus_tag="PLL28_06745"
     CDS             12734..13693
                     /locus_tag="PLL28_06745"
                     /inference="COORDINATES: protein motif:HMM:NF040769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelL-related redox protein"
                     /protein_id="HNF69057.1"
                     /translation="MELAILKNRLNATIAISAIYFLVWSVVVILFPNLLSSVVIGQGN
                     TPIVFWDFMSIITGVLGISLFIAAFNPHKHWLIILMASLFHLAMLAGFIVGYQHGIFS
                     SLYLPFLFFNHIIWLIPNSIVLFSVYRRSLDTDDMLLDAFNSKDYPLELFDTTDGRNI
                     GDMAAESPLLIVFLRHFGCPFCKESLLQLADYRKELEARGMTIVLVYMVNEHIAKEYL
                     SSYGLDDLPQVSDPEEIFYKRFQLRRGSFSQLFGLKVWSRWIELGFKKKLFNTKPEGD
                     VAQMPGIFLLNQGRVVQQFIHQSVADTPDYSVFLSHNAIRTND"
     gene            13697..15142
                     /locus_tag="PLL28_06750"
     CDS             13697..15142
                     /locus_tag="PLL28_06750"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNF69058.1"
                     /translation="MLIFVQIRVTAQRKYDLRFLFRRYVLIVVDVLLILTALFLYIRF
                     FHPSALQSYHTFYDNLLWVVMIVTLWFIYSYIFNLYKLSNVDRARLTIRNTVLTAALT
                     GLTYLFIPYLSPTLPYSRTPAFVLIGAMVVLVLLWRLCYAVFLKHPILNKKTLVVGAG
                     YTGREIVRTLLQDPAIYHKTAYKIYGFIDDDPAKTGKEYDGLKVLGKSDMLLKYAKRL
                     DVNEIILAVPEREDISAELYATLINLEHSGIQLMQATDIYEEQTGKVMIKKKGETYHI
                     TNPFSIVHKDNFYALLNRVINIICALLAGVVFILVLPFVWLGNLISSRGPLFYSQDRV
                     GENNKPFSIIKFRTMIVDAEKHTGPKFADRNDPRITRIGNILRKTRLDELPQCWNILR
                     GDINLIGPRPERDFFVQELSKQIPFFSLRSAVKPGLTGWAQVNYHYANNFDDTLVKLQ
                     YDMYYIKHRSLYLDMVIILKTIGVMIKFKGT"
     gene            15142..16380
                     /locus_tag="PLL28_06755"
     CDS             15142..16380
                     /locus_tag="PLL28_06755"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNF69059.1"
                     /translation="MEPEAQQVKSSIGHRIYFVLICGIALLIPFHNILPSPMLILTFL
                     SILITGEYATKWKRITSNKRFLLFAAIYIPAVYGYFLSSNTTIAGHDLQVRLYALLIP
                     IFFTLAGPLSKKELRRLLSCYVISSVLFALTAIVVAIWRYPDIGAQAFYYKELVSFTF
                     IHPSYAAMFLVFAVMLVSAELVTRWEEITSGNRIIYILLMLLLSLIVLLLTAKLAIAC
                     LFIVISLAVFVWGKKNYNTTKAILILISLNIAMFIAMMMLPYTRERMQMLLHYKETSY
                     SNSVDSREQIWHSAIQVASDHLIVGIGTGDAEDMLVSQYAANQFETGVQERYNCHNEY
                     LQVLVDTGLPGLIFFLLVLTGCFRLAFIDKNYLWMAFLLLFAINIITENMLRTQSGIV
                     FFSFFNALLGLHSGRKQAGV"
     gene            16490..16834
                     /locus_tag="PLL28_06760"
     CDS             16490..16834
                     /locus_tag="PLL28_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69060.1"
                     /translation="MKTLATLIILFSTTLVFAGNDKNISDREKATVINNVTQIVTNQD
                     VVSALGVSGQADVTLAVDEQGVIHVAEVQANNFLLEYHIRQSIEGAKMIVEDSLVGKT
                     INFFMNVVQPNE"
BASE COUNT         4754 a         3443 c         3513 g         5275 t
ORIGIN      
        1 gatattgcga aatatgatcc gaatgccata gcacaacctg ccgatctagt tgctgttgcg
       61 aaaaaactag ctgcaaatcc gaatatcgca aataaacgtt ggattactac gcaatacgac
      121 tccatggttg gtgtgcgcaa tacctcaaca aatttaaaaa gcgatgccag tttaattcgc
      181 atcatcggaa aaaacaaagg cattgccatg actacagatt gtaatgcgcg ttatgtgtat
      241 tccgatccgt atgttggtac gatgatagca gtgagtgaag ctgcaagaaa tattgtttgc
      301 agtggtgcga aacctgcagc cgtgacgaat tgtttgaatt ttggaaatcc atataatcct
      361 gaagtatact ggcagttcag caatgccatt cgcggtatgg gtgatgcatg tcgacagttt
      421 gataccccgg ttacaggcgg aaatgtgtcg ttctataatc agagtgaaga aggtcctgtg
      481 tatccaacgc ctaccattgg aatggttggt gtattggaag atttcaaata tcaaatgacg
      541 atggatttca tcaatgcggg aaatgtgatt ttattggttg gtaatgcaac tgatgatatg
      601 gcatgcagcg aatatttaaa agatattcat ggcatcacac aatcgcctgc tccgcgattt
      661 gatttgaagg aagaagcgag attgcaggat accatttaca atcttattca aaagcgacta
      721 atcgactcgg cacatgacgt gagtgaaggt ggattattta ccacgttgct ggaaagtgcg
      781 atggtgaatc agctgggatt tgaaatagaa acagatcacg gtattcgcag cgacgcttat
      841 ctttttggtg aaggtcaaag cagggtagtg gtgtcaacaa ccagcgataa ggtgtttgaa
      901 attgaagcga tatgtaaatc aaataatatt cctgtgaatg tgcttggaac ggtaacacag
      961 ggaagcatta ttattgatgg tgttcgttac ggtggcatat cagaatatcg cgaaatctac
     1021 gataatgcca taggaagata tatgcagcaa gaggttacgg tttaatgcca ttacggtatt
     1081 atcggaattg aatgtatata catatatttg tttgatgaaa cttttcggcg tgcccgattg
     1141 tttacagtat taatattcaa tgtcatgaaa aatctatggg tatacttagg gctgatatcc
     1201 ttatctgcat taggattcag cagttgtcag caatctgcta acggttccac caacgaacct
     1261 tcgggtatgc aaaaaagcgt tgaggccttt tcaccgggca cggaatggaa taactactgg
     1321 tatagcggaa aagcagagtt aaccagttat accctgcaac aaagtcgcta tggcgagata
     1381 catgccggta cagcggtgaa catatttgtt accgaagatt tttcaaaaag caagcatgtg
     1441 aaactcgacg atccatcgaa ggcaggtgca gataaattgc ctgtgttgaa gatgaatcag
     1501 tcgataaaat tcaatacagg aatatatcca tattctataa tgttaagcag ttttcagcct
     1561 gttgatgtaa atcagtttcc acatgcagtt aagatttccg gcacggttca ggaatggtgt
     1621 ggtatggcat attatcaatt agatcagcgc gatgaaaaat atcaggtaga ggtaagaagt
     1681 tattttgaat cggaaggtga taaagaattg tcgttgccgg caactgttca ggaagatgca
     1741 ctttggaaca tgataagaat tgctccagca aatttgccaa caggtgaaca aatgatattg
     1801 cccggtgcta cctatttgcg tttatcacat aagcctgtag aggccgttaa atcgcaattg
     1861 acacttcaaa taattgatgg tgtgcaggtg tataccatta aaatgccgtc actgaacaga
     1921 gaattaaaaa tcagatttga atctgcattc ccatatcgca ttttagggtg ggaagatact
     1981 tatcccggat ttgatggagt ttcattgact accactgcag ttcgcaataa agaaatgatc
     2041 atcgattatt ggcgtacgca caacaatgca gaccgtgttt tgcgagagca attaggtttg
     2101 cctaaggata cgcaataatt gttgagttta ttctacaaac ggaaggtcat gccggatggc
     2161 aaagcgcttt tgtgtttcaa caaaattatt cccttgttgc tgcaacatgt ttctattgga
     2221 gcttagaagt tcatgaattc tgatggagtc ttcaggataa taagtgctat cgcttaaaat
     2281 ggcataaata gtatcaaaaa ccacaaatga ttcgatataa ttttcaacca ttatgatagc
     2341 agcctgacgc aaggtatcgt cgccggtcaa tggttccgta ttggctaatg aatcttgcat
     2401 attcaataaa cgcattacgg tattatgttt cttgatcatg tactcttgca tgaaattatt
     2461 tgaattccac actttcattg ctctatcgct ttgttcgaca aatcgaatca ccttatcgtt
     2521 gtattcagta gcagcggcat atttgcgttt attgcaagat gtgcaaagag ccaaaaccaa
     2581 tccgaagagc accaggcttc tcataaattg acgtaatttt gttacaaatc taatcaaaat
     2641 gcaagccggt tccgataatc atctcaaaac tgttgtggta acaggcgcca tggggtttat
     2701 cggcagttgc ctggcggcgg cattacaccg aacgggcgca tggagggtgg tgatcagcga
     2761 tgattttaac agacctgata aaatgcaaaa tctgattgca ttgcaggatg ttttgaaggt
     2821 agaaagggat atgatagcag cgtgggttga tagtcagcct gccggcacca tatcctggat
     2881 attacatatt ggtgccagaa cagatactac cgaatttgat catgcaattt ttgatcgctt
     2941 aaacagaaat gcaacaaagg cattgtggga agtttgttgc aaacaacaaa taccgctcat
     3001 ttatgcaagt tctgcggcaa cttatggttc cggcgtacat ggttatgttg acaatcatgc
     3061 attaattccg gaattacaac ctttaaatcc atatgggtgg tcaaagcagg atatagatat
     3121 gtgggtgttg aaacagcaaa cgcaccctcc tttttggtat ggactgaaat tttttaatgt
     3181 gtacggtccg aatgaatatc acaaaggccg catggcatct gtaatatttc atgcatacaa
     3241 tcaaatgcag gcaaccggta ccatcaaatt attcagaagt cataacgatg cctatcgcga
     3301 cgggtatcaa ttacgcgatt ttgtttatgt gttggatgtt gttgatgtca ttcaatggtt
     3361 aatgcataat aaacctgcaa gtggaattta taacctcggc accggtaaag cagaaccatt
     3421 ctatcaattg gctgcatcta ctgcagaggc attaaatatt ccattgcaaa tcgattggat
     3481 agatattccg gaagatattc gcgataaata ccaatatttc accgaagccg atatgtcgaa
     3541 attgcgcaaa gctgggtata cacgcgcttt cagcagtctt gaagcaggtg ttgccgatta
     3601 tgtaaaaaat tacctcgtta gaggaaatta ctattgagta ttaccattct acaataatgc
     3661 cgatacttgg taatacagtt ccggtttcat tttccaaatc ataagtcaga tatctggtcg
     3721 gatcatcagg attgataacc ggattgccgt ctgcatcttt tactacatca atatacggtt
     3781 gaaaggtagt ttggaaatta tatacattct gcacatctac atacacatca agattccatt
     3841 tatcgaagaa ccatttttta tcaacgcgca tatccaattg atgatatgga tcaagtctct
     3901 ctgcattcaa tttactgaaa tcaggaacgc cttgacgata aatatcccaa acataggtga
     3961 gtgatgagta ttcctgatca taaggcgtat atggcaagcc gcctgagtat cgccatttaa
     4021 gaccgagttc ccagtttttc ttaaattttt tgccggctgt cattgaaatt attgtgcgat
     4081 tatcccatga tgaggcagta tatacattat cgccattttc aaattcactc accacatagg
     4141 tcagcgatat gatatagtag taatttttat ttaatcgctg ctgtgcaaga aattccacgc
     4201 catagctgcg accattgctt gtggatgtta ccggttcatc gccaactaca ccaaaatcgg
     4261 caccttgatt ggcaattgaa atagaatcgc ggagactgaa tggatatcga gcatagtttt
     4321 tataaaatcc ttcaagagta aatcgcagat aagattccgg tctgtattcc aatcccgcaa
     4381 cgagatgtga tgactggatg taacggagat cgttttcttt atttacaaaa ttattttcac
     4441 cttccgcata tcccaatacc gtgtaggccg gcatttgata ataaatacca ctgttgaagt
     4501 ttaaactcca tttgtcagta agtccataac ttgccgataa tcgcggagac agctgctgta
     4561 aagggtttgc catggaactg ctgtagtcat tgccatccag gcgaagtccc aatgataagg
     4621 taagtcggta atcgagaagg gtaatgcttg cttgtccgaa agcaccatac ttattgacaa
     4681 ataatttcga tgtgtaatct attgttgtga tgtcggttcc cagaggtaat aattgatagg
     4741 tggaattggt atatctggct aattcatagt ttaatccgcc tttgattctg aatcgaccaa
     4801 tattcgctgt ttcctcgtag cgaaatttat tttctgattc ctgactgcca taattatata
     4861 tcaggttttc cggattggaa tcgtcgttat tctcatattt ataagcttcg ttattcagca
     4921 tatttctgct ggcgacaaca gcagaatatc ctttttcgcg ataatgtttg taaacaacgc
     4981 ctaatgtata gttccattgc gtttgaaccg gaaggttacc taatacatat tgttgaaacg
     5041 cacttgtatc gtcttcgaga tttaaatcga actgatcaat ggcgccgaga ccgataatgg
     5101 tgatttcatt ttgcttatcg agttttattt tctgcttgta ttgaaagtcg ttgtaggtgg
     5161 gaaggaatgg taatccgata gcgccaaaaa ggaattgcag ataacttctg cgtgccgata
     5221 acaatacagt tgatttttca ccgattggac cttccatggt gataccgaga tcgcttgagc
     5281 cgactgtaaa tgttcctccc catttatctg atctgccatc gcgttgttgg aatgacatta
     5341 cactgctgag tgcattgcca taattcaccg gaaatgcacc ggcataaaat tctacttcac
     5401 gaataaaatc tacatttatc aatcccaccg gtccgccact ggaaccttgc gttgcgaagt
     5461 gattgatatt tggaacttca acatcatcta agtaaaatct gttttcattt ggagcccctc
     5521 cgcgtatgat gatatcattt ctaaaagatg gcgtataagt aacacccgga agtgtctgta
     5581 tcactttcga aatatcgcga ccgccaccgg ggtttcgttg aatttcattg gcgccaattg
     5641 tccggagtga caggggagac tcttcggttt tgtcgctaac gggtgctttc acttcaatgg
     5701 catccaatac attgaggtct tcctgcattt ggatgttgat gaatacaggc tttgcattgg
     5761 taacttgaac ttcagggatg gcaacttttt taaaaccgat gaaagtgcat tcgatgttat
     5821 ataatcctgg ttctatacct tctatggaat aattccctag gtcgtcagaa acagcgccgt
     5881 atgaagttcc tgctaaaaca actgttgcga aaggtacagg ttcgttgttg atgctattgg
     5941 ttattgtgcc ggcgattttg ccattttggg ggaaggcgtg gatgctgata gaaatcagca
     6001 acaagaaggt aatggagcgc ataatttcgt taaacaaagg tacgggggtt ttgtttaaaa
     6061 aattatggat atatagggga aaatataaat tgtgtttgtg ggtgtttgtg gtttgtgttt
     6121 ttgtggtttg tgttttttca cgcggaggcg cggagacgca gaggggtggt gtgtggtgtg
     6181 tgtttgcttc gcgctaagac gcaaagttgc aaagccgcaa aacatgcatt gcaaccccaa
     6241 aactcaccca cccgcctact cggcgaggta aactcaccca ctcacccact cacccactca
     6301 cctttctcct ttctcatttc tcatttctca tttctcctct cctcccactg ttcttgtgtc
     6361 atcacgcatc tgaaacctga gtgatcttga ccggtatcgt agcttgtagc cattcgtgcg
     6421 gtaaggcggt agcttgagca gtaaacttca ttacataaaa atgagccacc tcgtttggtg
     6481 tgcacttcat taaaaggttg attgggatcg taccaatgtt ttggtccttg aggatttacc
     6541 aaagtgcctt ctaatttaca ggcagcataa tattctgaat gataataatc gcttacccat
     6601 tcccaaacat ttccagccac atcatataat ccataaccat ttggctcgta agattttaca
     6661 ggagctgttg ttacataacc atcttcatgc gtattgttgt agggaaagtc gcctgtccat
     6721 gaattggtat gtttcgccaa ttgtttatca tttccccagg catatatttg atcggtagta
     6781 ccgccgcggg cagcaaattc ccattccgct tcggtaggta atctttttcc ggcccattta
     6841 cagtacgcaa ctgcatcatc ccaactaacc tgaacaacag gatgattgtt ttttccttta
     6901 atagtacttg tgtcaccctg tggttttcgc cagcttgcac cgggaacaaa tttccaccaa
     6961 ttgagaaaat tattcagatc aacagggtga tcggtggagg taaaaaccat gctggctggt
     7021 tgcaataaat catcagcagg tttgggtgtt ccgggaggaa cttgtttttt caactcttcc
     7081 cagtcgattg gccgttcagc agttgtaaga taaccggtag ctttaacgaa ttcggcaaac
     7141 tgtgcatttg tcacttcggt aatatccatc caaaagccat caacttttac aggatgtgca
     7201 ggtgtctcat cagagcggcg ttgcggatcg gtgtcagtgc ccatcatgta tgttccgccc
     7261 ggtatccata ccatccccgg aggtccgtca gcaggttgat gtagggtatc gacagtaaca
     7321 acttctgtgt cggtggtatt tgtctccgat acttgctgcg gccctgaatc cgcagaattt
     7381 agtttactgc aggaaacagc aaggatgctc attccgacga tcaagctgtg gatggtccat
     7441 ttctgtttca tattacaaat gtaagacgtg aattactttg ttttggtttc ataggggaag
     7501 ctaattattc gataataatt gatttactga tgttcaatgt tgaagttttc accgacatgg
     7561 tatatacgcc ggccggtaaa tcacgtgcat caatataaat cgtgttagaa cccggtatca
     7621 gattagcggc agactgataa acaacctgac caacagtatt caataattgt aattctgcat
     7681 ttaccgcttc atcgcaattg atgatggcat aacattttcc actattcgga tttggatata
     7741 tttgcacgga aatattctct tcatgcgaag tgataccttc tttttctaca gcgatattaa
     7801 tattatcagc atacattgct tgtccccaat aaccgatatt tctaaatacc aacattaatg
     7861 aaacatcatc tatgtatggc gttaaatcaa ttgtttctgt tcgccattca tctgcggcag
     7921 gataaaatac atcagcagta tatgcaggtc cggttgccaa tgtattgcca cctttgtaat
     7981 aaattagatc taatgtagca ccgcaatcat tcgacacata aacggcaagt gtatccatat
     8041 aaattcctcc gtagggacga taagcaacat cgaatgtcaa ctgtgccgta attgcagcat
     8101 ctatatcata ttgtgcggtg atttcatcaa catgatctcc ttgcaagtct gtccagtaat
     8161 tattaaaaaa catgctgtaa gtgccaacat catatgcact tgcatcttca gctatccccc
     8221 agccaccgct ggttccgcca tgccatcctt caggaaattg tgcggtctcg aaattttctg
     8281 acaaaggtaa tgttgacgga gccaatgaac tcacaattgc cgattttgtg atcgattgca
     8341 cttcggcacc atctgtaatg gttaacgttg catcgaatac gccttcggta ggataggtga
     8401 caacaggatt ttgttctgat gaagtagccg gaatacctcc cggaaattcc catgcataag
     8461 ttgctcctgc acctgccgtt gaatgatcta catatttaat ttgatctcct gcgcaataaa
     8521 aattcggttt ctctgcagaa aaatctgcta tcaatgttga tggctcatat aaaggagctt
     8581 cccatatacc gatattccaa cttcctaatc tgattttgtc gtcgcgataa aatggaatag
     8641 ccttgagcgg atctatcacc aatggcaggt cattaccatg ctgcagccaa tcgataaatg
     8701 tatttgtgcg ataatacact ttcgcttcgc gcatgaatac gtaaacaccg ccatcagtac
     8761 caaactgatg cactattgcc gaaggatgca tatcatttaa cgtagccgtt gtgatattaa
     8821 cccatgaatc accaccattt atagagtggt ataccttgct tccgttagga ccgtatttat
     8881 aggcaatcca taattcatcg gcattatcac cgcttaatgt aaatatgagg tcgcgtttag
     8941 cttgcggaag tgttaagttg gtccatgtta atcctctatc cgtacttttc cagaggtaac
     9001 aatttgttga accgatattt tgtgtgcaat aaataatgtt tgtatttact ctgctttgtt
     9061 caacccacaa cactttatta ttcgcattag tgccaaattc atgcagcggg gtaaatccac
     9121 cgccaccgtc tgtgcttttc catataatat tatcacgacc catatatgcg tgatcaaaat
     9181 aatcccagtc aaattgtatg cgtgaacttg aattatacca atagctttca tttggtgcat
     9241 tcgaaaatgc gaggtgttct gcaatacctt ctatctcatc cggtaatttg ataccaccaa
     9301 tatcgctgaa catacagata ttctcatcac tttgctttac atatccggat gcagcctcgc
     9361 caccaccgag tgccaagaat tcgccttcag gataattttc gtgataggcg ccgttcccat
     9421 tgtgatatct tcctcccacc atcatatctt cctgccatcc ctgatcaaat ccccagaaat
     9481 tacttccgcg caagccatta cttctgcttt catgcgattc tacccagttg gttgtataat
     9541 ttacaccacc atctgttgcc atccatatct cttctgttgt aggtgatgtt ttataaattt
     9601 ttgtctcctg attatccgga tgataataag gcagatatcc taaatatcca ccaaccggat
     9661 cataagagac tgctccatct tcgctcttcc acaaattcaa tccgcctatg agcaatttat
     9721 tttcatccag ttgtgaaaca gcaattgttg tgttgtaata aatctgatca taatcgctag
     9781 aatgtccatc gaaatccatg aggtttggat gtgtgtcaac attatacggc attccaatta
     9841 aaccgtgcgg aaaactccaa ctttcaccac tgtcattact catataaaca cctatggtgc
     9901 cgcgtaattg taatcctgcg tctgcatcgc tttcaccaac cagtaataca tatactttat
     9961 cgggattggc ctcggtaaca cctattctgc ctccaaggct ctcaattttt cccatatcgg
    10021 cagcaggaac cgtaaaccag ccatcgctgt aagtggtgaa tgttgcaccg taatcaaatg
    10081 attttttgaa atagggaatt tgtgttgtcg ggtcgtattg taatgtgtaa attattgctg
    10141 cattatcagg ttttacttcc accgacatac aaaaatgatc taatatttct gtccagttac
    10201 tgccgccatc agtacttctg aaaaatcctt gctcacatcc tgcataaaca atattgctgt
    10261 tttccggatt aaacacaata tcataagcgg tgatgtaaat cgcctggaaa gcagcatcac
    10321 ccataatatt ccaggtaata ccaccatctg ttgtgcgcca taattcacct tcagatgaag
    10381 cgattacaat atcaggatta aatggatcaa ccgccagtgc tcttaccgta ttgatataat
    10441 atccatgcgt catatgcgtc caggtcattg cctgatctgt ggttttatat ataccaccgg
    10501 tttcacctcc gcaataggca atatttggat tacttttgct gcgatcaaaa ctataggtat
    10561 tcatgtgatc tgacaccgga atttgagttg cgccggcatc atattttgta gtaaaatgta
    10621 tgtcgggtcc tgcgaatgac catgttgtac ttctgcttcc ttcatctatg acttgcgagc
    10681 gtgttctgct atttatttcg cgcatcaatt gcatgtgcgt tgggaaatgc acatcgccat
    10741 tttccaatac atatggtctt gctaatatcg cccagcgttg aaagtatcgg gtgtaattat
    10801 tttcctcaaa ttcatgcgtt ttataatagg tgtcgaatgc atcttgaatt gcataagcat
    10861 tcggtgtttc agtatacatg agttgcaccc attgcggcgc atcagctgtt aatgcaggtt
    10921 ttggctgatc aagctgaccg tataggaaca ttggaattat cagcaacgag agtgtcagat
    10981 atcgcaggtt atacattaaa aatattttaa ttatggtttg atcttatttg gatggcgtac
    11041 taatatatca ctgccgcatg ctaataatac tgagtgcggt gttgcaacat tcagaaatga
    11101 aaatgtatca ccatataaag tggcaaaatc tacttgtatg gtatgcttga tgatatctct
    11161 ggtttcccat cgtggatgtt caacagcata ttcataggtg gagttattat tgatggaagc
    11221 atacccccaa taatgctctg taataaattc ggatatgtct ccgggtatca ataatttctt
    11281 ttcaggtgcc agcttagctt ccatgctata tttcttgccc caagtataac cgacaaaatc
    11341 atgctcattg gtgcgaaaca atccaccgcc tgttggcatt gttacataat tttcgttgta
    11401 taaggtattt gcaaaaaatg aaattaaatg tcgcggaaca atttctttaa tgaatacgac
    11461 acctcgcttc aattgattat tttctattct tttcacatac atgcgaagat ttatctccac
    11521 aaaattttta tgccagggga aagcaatttt tttgacgcgc gtattattaa attcaaacgc
    11581 caccaaactt aaaaagcagt gaccatcaaa agtatcatgt gcagttccat atggaagcca
    11641 agggtcaaga atctccgggt ctaccacata atttgccatg agtagatttt gccatttggc
    11701 ggtgagaaaa acaggctttg tggtcattat acaaagatac caatgtgatt ggactgtatg
    11761 ggcaaaaaaa agccaccccg aaggatggct ttgtgtcagt agaatatcat tatttatttg
    11821 atttccattt attcagttcc ttctcaatac cttgagagaa accggtgttt ttggcttcat
    11881 ttgacaatgt gagggccttt tcagcggtct caatagcttc tttattcatg ccgtttttag
    11941 cataagcttt tgccagtgta aacaagttcc agtagcgttc gcgctgagct actgattttt
    12001 tagcccactc aagtgcttgt ttggcgtcca ggttattatc gagataaaat tcagctgcat
    12061 cattatacaa gctcatcgta ttttctgctg cagcgattgc ctcagtaata ttggttttgc
    12121 cttgttccag gaaatcgtta gtcagttcaa aactgatcat ggttgtttcc cagtaaattt
    12181 caagaacggc gctgttatcc ttaatattcg aaaaacctat tgtaaacgat tctgttgatg
    12241 tgtgcgactg cactttcacc tgaaaacgca ttgcatcttc ttcttgttta tagccgcctg
    12301 ttccccataa ttctgtgttt ttattgatga ttacggtcca ctctttctct ccgggaattg
    12361 taaaaagtga atatgcaccg gcaggtaaaa tttgaccatt gatagccaca tttccttcca
    12421 atttcaactc agtagcctta ttggcacctg ttctccacat ttcgccgtaa ggaacaagag
    12481 agccaaaaat ggtgcgaccc ttcatagatg ggcgtgaata ggttaccgta gcctgtgcca
    12541 gaccaaattt ttggcttacc tgagcccagg gactaggttg aggaagctct aactcctgag
    12601 cggaaagctt ctgtgtggct gccgtgataa gtaacagacc gatagtaaga atttttttca
    12661 ttgagtatct atttttgttg atgcaaaggt aaacaaatca aacccctgct tgtttactcc
    12721 ctgatatcca aatatggaac tagctattct caaaaatagg ctcaatgcca ctattgccat
    12781 cagtgcgatc tattttctgg tgtggagtgt agtggttatt ctattcccaa acctgctctc
    12841 cagcgtggtt atagggcaag gtaatacccc aattgttttt tgggatttca tgagtattat
    12901 caccggcgtc ttaggtatta gcctgtttat tgcagccttc aacccccata aacactggtt
    12961 gatcattcta atggcaagcc ttttccatct ggcaatgttg gccggattca ttgtcggata
    13021 ccaacacgga atcttcagca gcctttacct tccttttttg ttttttaatc atatcatttg
    13081 gctgattcct aattccatag tgctcttcag tgtttaccgc agaagcctcg ataccgacga
    13141 tatgctcctg gatgcgttta acagcaagga ttatccgctt gagttgtttg acaccacaga
    13201 tggacgaaat attggcgata tggcggcgga atcaccactt ttaattgttt ttcttcgcca
    13261 ttttgggtgt cccttctgca aagaaagcct cctccagctg gctgattacc gcaaggagct
    13321 tgaagcccgg ggtatgacaa ttgtgctcgt ttatatggtg aatgagcata tcgcaaagga
    13381 atatttgagc agctatggcc ttgatgatct gcctcaggta agcgatccgg aagagatatt
    13441 ttataagcgc tttcagttgc gcagaggcag cttttcgcag ttgtttgggt tgaaagtgtg
    13501 gtcgcggtgg atagagcttg gcttcaaaaa gaagctcttt aataccaaac cagaaggaga
    13561 tgtagcccaa atgcccggaa tttttctgct caatcaaggt cgggtggtgc agcaatttat
    13621 tcatcaatcc gtagccgata cccctgatta ttcagtcttc ttgtcgcata acgccattcg
    13681 caccaacgat tgacaaatgc ttatttttgt gcagattcgc gtgactgcac aaagaaaata
    13741 tgacctccgc tttctgttca gaaggtatgt cctgatcgtt gtggatgtat tgctgattct
    13801 taccgcatta tttctgtata ttcggttttt tcatccttca gcactccagt cgtatcatac
    13861 gttttatgac aatctcctct gggtagtaat gatcgttacc ctttggttta tttattccta
    13921 cattttcaat ctctataaat taagtaatgt cgatcgcgcc aggctgacca tcagaaatac
    13981 tgtgctcaca gctgcgctaa ccggattgac ttatttattc attccttatt taagtcctac
    14041 gctgccatat tcaagaaccc ctgcatttgt attaatcggt gccatggtgg ttttggtttt
    14101 attatggcga ttgtgctatg ctgtatttct gaagcatccg attctaaata aaaaaacgct
    14161 ggttgtagga gcagggtata ccggtcgtga gatcgtgcga acacttttgc aggatcctgc
    14221 aatctatcac aagacagcat acaaaattta tggatttatt gatgacgacc ctgcaaaaac
    14281 agggaaggaa tatgatggat tgaaagtatt aggtaaatcg gatatgttgc tcaagtacgc
    14341 aaagcgattg gatgtgaatg aaatcattct cgctgtgcct gagcgggaag atatcagtgc
    14401 cgaattatac gccactctta ttaatctcga acactccggt attcaactga tgcaagccac
    14461 tgatatctac gaggagcaaa cagggaaggt gatgattaaa aagaagggag aaacttacca
    14521 catcaccaat ccattcagca tagtacacaa agacaatttt tatgcattgc tcaatcgcgt
    14581 gatcaatatt atttgtgcct tactggcagg tgtagtattt atcttggtgt tgcctttcgt
    14641 ttggcttggc aacttgatta gcagtcgcgg ccctttattt tattctcagg atagagtagg
    14701 ggagaataat aaaccattta gcatcattaa gttccgcacc atgattgtgg atgctgaaaa
    14761 acatacgggt cccaaatttg ccgatcgcaa tgatcctcgt attacacgaa ttggcaatat
    14821 tctgagaaaa acgcgtttgg atgaattgcc gcaatgctgg aatattttgc gcggcgacat
    14881 caacctgata ggcccaagac cggaacgcga tttttttgtg caggaattga gtaagcagat
    14941 tccatttttc tcgctgcgat cggcggtaaa acccggactt accggatggg cacaggtgaa
    15001 ctatcattat gcaaataatt tcgatgatac actcgtgaaa ttgcaatatg atatgtacta
    15061 tatcaaacat cgcagcctat atctggatat ggtgatcatt ctgaaaacta ttggcgtgat
    15121 gatcaaattc aaaggtacct gatggagcct gaagcgcaac aagtaaaatc ctctatcggt
    15181 catcgcattt attttgtgct gatatgcggt attgcattat taattccatt tcataatatc
    15241 ctgccatcgc caatgttgat attgacgttt ctgtcgattt tgatcaccgg tgagtatgcc
    15301 actaagtgga aaaggattac aagcaataaa cgtttcttgt tattcgcggc aatttatatt
    15361 cctgctgttt atggatattt tctttcttca aatacaacaa tcgccgggca tgacctgcaa
    15421 gtgcgtttgt atgcattact gatacctata tttttcaccc tcgccggtcc gctttcgaaa
    15481 aaagaattgc gaagattatt atcatgttat gttatttcaa gtgtattgtt tgcattaaca
    15541 gctatagtag ttgctatatg gcgatatccg gatatcggcg cacaggcatt ttattataaa
    15601 gaacttgtat cgttcacatt tattcatccg tcatatgcag caatgtttct tgtttttgca
    15661 gtgatgttgg tgtcggcgga attggttact aggtgggaag aaattacctc aggcaatcgc
    15721 ataatttata tactattaat gttattgctt tccctgattg tattgctgct tactgcaaaa
    15781 ctggcgatag catgcttatt tatagtaatt tctctggcag tttttgtttg ggggaagaaa
    15841 aattataata cgacaaaggc aatattgatt ttaataagtc tgaacattgc gatgtttatt
    15901 gctatgatga tgttgcctta cacgcgagaa cgcatgcaga tgctattaca ttacaaagag
    15961 accagctata gtaatagtgt cgattcccgt gaacaaattt ggcactccgc tattcaggtt
    16021 gcatccgatc atttgatcgt aggcataggt acaggcgatg ctgaagacat gttagtatca
    16081 cagtatgctg cgaatcaatt tgaaaccggt gttcaagagc gatataattg tcataatgaa
    16141 tatctgcagg tactcgtaga taccggtttg ccggggctga tcttcttttt gcttgtatta
    16201 acaggctgct ttcgattggc ttttatcgat aaaaactacc tctggatggc ctttcttttg
    16261 ttgtttgcca tcaatatcat caccgaaaac atgctccgaa ctcagagtgg catcgttttt
    16321 tttagtttct tcaatgcctt gctgggactg cattccgggc gaaaacaggc gggcgtttag
    16381 cactttttaa caattttttt tgcggcttct gtaacttttt atgggggcag ttcgccatat
    16441 gttcgtattt atcaaaacaa catcaaacaa actatacaaa cagaatatta tgaaaacctt
    16501 agctacactc atcatcctct tcagcacaac acttgtattc gcaggaaacg ataaaaatat
    16561 cagcgatcgc gaaaaagcaa ctgtaattaa taatgtaacg caaattgtta cgaatcaaga
    16621 cgtggtaagc gcacttggtg taagcggtca ggcagatgta acacttgctg ttgacgaaca
    16681 aggtgtgatt cacgttgctg aagtgcaggc aaataacttt cttttggaat accatatccg
    16741 ccaaagtatt gaaggtgcaa aaatgattgt agaagactcg ctggtaggta aaactatcaa
    16801 cttcttcatg aatgtagtgc aacctaacga atgatcatga cgatggataa tatacggtta
    16861 aagtaacgtc atccactttg accgtcaaga ccctctacgg agggtttttt ttattatatt
    16921 ggtatcaggt atcaagtatc aagtatcagg tatcaagtat caggtatcaa gtatcaggta
    16981 aaaaa
//
LOCUS       DAOQHY010000062        16759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1599_length_16759_cov_4.814535, whole genome shotgun sequence.
ACCESSION   DAOQHY010000062 DAOQHY010000000
VERSION     DAOQHY010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16759
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1599_length_16759_cov_4.814535"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="PLL28_06765"
     CDS             <1..673
                     /locus_tag="PLL28_06765"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HNF69061.1"
                     /translation="VNGEKAYVAPEQQKSYDVTFKAGLGVDAAAGILLKMKLNSMDFE
                     DTVNVTPNKIMITANMSIADSKLLAKNKQVESVILNHFDRMVAGYIGLFPNNPNQPLN
                     SVDDFSPIYIPKKGATVKIDANSYYNYKRIIEAYEHNTVTLDKQGRPYIDGKLLTEYT
                     FKQNYYWMMGDNRHRSYDSRYWGYVPEDHIVGKPVLLWFSWDKTLPLSERIANIRWDR
                     MMHFK"
     gene            678..1253
                     /locus_tag="PLL28_06770"
     CDS             678..1253
                     /locus_tag="PLL28_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69062.1"
                     /translation="MQIKIGALLAISILMLHACKESADNEDTAAIENVGEKGGDNSLQ
                     ELIDKEATTPDVVKQASGDQVLYNERFDYTITIPGFYTIKEKSNNGDGYFISSGDKDV
                     DIRVYGENIADNQVMAEMELNTCESKTSFQFSNGYPGILCMQSDDRYYYYDTPKTRVT
                     VYVHAPTRWFDRNNKLLDIMVKSLVVGKGGF"
     gene            1374..1835
                     /locus_tag="PLL28_06775"
     CDS             1374..1835
                     /locus_tag="PLL28_06775"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNF69063.1"
                     /translation="MQIIFISVGKTSEKWVSDACSTYVKRLGKYVRFHWHETTDIKSL
                     PPEEMKLREFAAIKKYIEQGDHVVLLDERGAAYSSVGFSEFITRKQLENRKRLVFVLG
                     GAHGFHEDAYSMANETLRLSDMTFPHQMVRIIFLEQLYRAFTIINREPYHH"
     gene            1839..2477
                     /locus_tag="PLL28_06780"
     CDS             1839..2477
                     /locus_tag="PLL28_06780"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNF69064.1"
                     /translation="MENVTVTAFIILLTAISSILAFNNSVMRGSALFYPHAMHQRGEW
                     YRFITSGFIHADYIHLIVNMFVLWSFGSILEERLLPLVFQGYGRIVYVGIYLLGMIVS
                     DVPSYFKHRTDATYRGLGASGAVAAVVFASILISPFDGEIGFFLIPGLHVKPFVFGII
                     YLIYSAYMGRRGGDNVNHDAHFYGALFGLIFPGLIKPELFISLYHQILQQWT"
     gene            2468..3493
                     /locus_tag="PLL28_06785"
     CDS             2468..3493
                     /locus_tag="PLL28_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HNF69065.1"
                     /translation="MDIIDLRSDTVTKPSVEMRAAMASAQVGDDVFMEDPSILELEQK
                     ICAMFGTEMALLTPTATMSNQIALKILTHPLDEIICDRTAHIYSYEVGGYAFHSGCSI
                     RYVEGDRGVFSATDVIKQINPDDVHKPITRVVSIENTVNRGGGRIYPIETIAEIAAVC
                     REKGLSFHLDGSRLFNALVASGADAKQFGELFDTITLCFSKGLGCPAGSILTGKAIHL
                     QKARRVRKVMGGGMRQAGVLAAAGLYALEHNISRLAEDHQRAAETGNALNKLSYVESV
                     LPVETNIVIFRLRENISDEKFIQYLNHLGIKAFAIGDNWVRFVFHLDIISGMEEKIAS
                     ALINFNA"
     gene            complement(3490..3978)
                     /locus_tag="PLL28_06790"
     CDS             complement(3490..3978)
                     /locus_tag="PLL28_06790"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HNF69066.1"
                     /translation="MIAQLLNLAEDHLHGISHNIPELNDLDSNQVTSVTSETVVNTIV
                     QQARKGNVDALREMLSGSETAKDHEVIRQLENPVINQLQSKLNISDQTAKQLAVIALP
                     VVMNLLNGKVKHAQTGGFDINDAIRGLNGNSGMLKGIFNLFGGNKSSTKTINSILQNL
                     IG"
     gene            complement(4041..5705)
                     /locus_tag="PLL28_06795"
     CDS             complement(4041..5705)
                     /locus_tag="PLL28_06795"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNF69067.1"
                     /translation="MKSTITAIFLIIFGLQSQAQLFDPEHAYTRADTLRGSLRAERTC
                     YDVLTYDLAVKVDIANKAIEGSNTINFKAVSDFQRLQIDLFEELSITKIIFEGKDIQF
                     KREFAAVWVDFPKVIKGGTQGEFTVYYSGHPRIAKMAPWDGGISWNTDEKGNPWVGVS
                     CQGLGASVWWPNKDHLSDEPESMRIAITAPDTLMAVANGNLISTSIPEKGWKTWHWAV
                     TYPINNYNVTMNIGKYVHFEDTYTNVDGSTYSLDYYVLEYNVDKAKKQFEQVKPMLKA
                     YEQYLGAYPFPRDGYALVETPYVGMEHQSAIAYGNGYQTGYSGFDYSGIGLDFDYIII
                     HETAHEWWGNNVSMKDLADMWIHEGFGTYSEAIYVEALYGNATAMNYINAMKQKVRND
                     EPIIGDYNVNNEGSGDMYHKGAIFLNSLRTLTNNDQLWWSIIKGIQKDFAQQTVTTEQ
                     IERYISRKAGKDFSKEFDQYLRHKDLPKFEYKLTPEAGAVKLEYRWSADVPEFDMPLR
                     YMGADGAWKWLKPTTDWQETSLDLPNIKAFKLDTTHFYFEERMVKK"
     gene            5915..6322
                     /locus_tag="PLL28_06800"
     CDS             5915..6322
                     /locus_tag="PLL28_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69068.1"
                     /translation="MFNIRNSSLALLLPIAIGFLASGCTSLPGDGGTSTIHGKIYVER
                     YNEFGTLYQDYYGPDERVYIIYGDNIGYDDEVKTSFDGTYKFPFLRKGTYTIYAYSDC
                     LTCDGNTEARSVQVVITENNQDIEVEDIVIEAR"
     gene            6377..7075
                     /locus_tag="PLL28_06805"
     CDS             6377..7075
                     /locus_tag="PLL28_06805"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HNF69069.1"
                     /translation="MDKVELMNRKDTKYFFDRKKLIEILDLLQHDYRVLEIGGHRIFN
                     YRNIYFDTPEFECYHMHHNKRVNRYKIRIRQYVESNLNFLEIKFKINTGRTIKSRIKI
                     AEFSPILSEEAKRFISEKTNFDPEQLRPTLENDFRRFTLVSNSMRERATIDTDLRFKT
                     PNGQMQLSNVCIAELKLDSSSSGSPFKLAMRDNFCPEARISKYSVGAAFMYSDLKQNN
                     FKFKKLQINRIENV"
     gene            7068..7748
                     /locus_tag="PLL28_06810"
     CDS             7068..7748
                     /locus_tag="PLL28_06810"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HNF69070.1"
                     /translation="MFSTLIQAAEEQTKFLDLKFFDDDVYDLILRSFFNFVIVFILVK
                     FTYRSDRKNKNYAFSFYIFSMLVFFLCYLLSGIKLEMGFAFGLFAIFSILRYRTISIP
                     MKEMTYLFLVIAVAVINALTTKKVSFVELLFTNVSLIAATYFLERLWYREGLSEQIVE
                     YEKIENIKPERRAEMLEDLRIRTGLDIRSFEILNTDFLRDMAKVRIFFKPTPHFGEED
                     MEHDDDDD"
     gene            7771..8373
                     /locus_tag="PLL28_06815"
     CDS             7771..8373
                     /locus_tag="PLL28_06815"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HNF69071.1"
                     /translation="MLTLSVCAKAQYTDGGVWLYSGINHEVVKDLKLEFGEELRYNIS
                     TDNPYQYNTHFSAAYKLNKHWKIGADYRYSVREGLNTNRYGAAITYKQSIHDLDISLR
                     SRFLYSVVLDGAEGTAWRNKLGCDYKINKDFQVGVSGELFYAMSNELDQFDNYRLQAG
                     LTYSPNKYHSLDVGYIYDHEFNVNNPGSMHVANIGYVFSF"
     gene            8665..9576
                     /gene="hemF"
                     /locus_tag="PLL28_06820"
     CDS             8665..9576
                     /gene="hemF"
                     /locus_tag="PLL28_06820"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF003727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HNF69072.1"
                     /translation="MRNVDRFAISDWFKGLQDHICDEIASTDGKGVFEQELWQREGGG
                     GGRTRIIQNANILEKGGVNYSEVHGVLPSRIADAMQVSGHDFFATGVSIVMHPFSPMV
                     PIIHMNVRYFEVSSGEKWFGGGIDLTPIYIDKTQAAVFHRKLKEVCDRHHENYYAEFK
                     QWADDYFYIKHRKETRGIGGIFFDRLQATEYISLSDRFEFVKQVGMLFSEIYTRLMQE
                     NKDIPYGKPEKLFQALRRGRYVEFNLVYDKGTKFGLDTDGRAESILMSLPPEAGWPYN
                     YKPIAGSREEQTIDLLRKGVEWASADI"
     gene            complement(9573..9989)
                     /locus_tag="PLL28_06825"
     CDS             complement(9573..9989)
                     /locus_tag="PLL28_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69073.1"
                     /translation="MFAMLLAACVKPPVYPIEPYIEVVSINQDNFDELDEDSLSITLY
                     FEDGDGDIGSEDEVNMFWEDSRAPGFQIPFKIPYVQSQGNTKAISGTITTYYPISFCI
                     DDSDNIDTFYYKIFIVDRAGHISNTDSTQLIFLHCL"
     gene            10062..11045
                     /locus_tag="PLL28_06830"
     CDS             10062..11045
                     /locus_tag="PLL28_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HNF69074.1"
                     /translation="MQDIARFTESIFEVTADTFAGKALAIFNYQAKENAVYRDFLQYL
                     DISPSSVKSISDIPFLPISAFKHHKVVCGGLETDTYFESSGTTDSQRSRHYIVDRMTY
                     ENSYLSSFRQVYGDPINYRILALLPSYLERSNASLVDMCRGLIERSGHPESGFYLHDL
                     QSLSQVLSNDTGIKTILIGVSFALLDLAEQFPQSLTNTIIMETGGMKGRRSEITREAL
                     HDVLRNAFNVDVVHSEYGMTELFSQAYSTGYGIFQCPPWMRIIIRDSEDPFQLLETGK
                     TGGVNIIDLANLHSCSFIATDDLGSIQQDNRFSIAGRFDNSDIRGCNLMTL"
     gene            complement(11059..11604)
                     /locus_tag="PLL28_06835"
     CDS             complement(11059..11604)
                     /locus_tag="PLL28_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNF69075.1"
                     /translation="MQKTPNAALSRLSVEQYKNADKHPFVVVLDNVRSQNNVGSVFRT
                     ADAFLLRGICLCGITATPPHRDIEKTALGATESVYWEYFDHTVDAIKKLKELGYGIVA
                     VEQTSGSVALQDFDPSSFEKIAFVFGHEVMGVSEDALALCDASIEIPQFGTKHSLNIA
                     VSAGIVLWDIVMKHKGQSASK"
     gene            complement(11655..12167)
                     /locus_tag="PLL28_06840"
     CDS             complement(11655..12167)
                     /locus_tag="PLL28_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF69076.1"
                     /translation="MKDKQFKVFLVDDDTKHLIMLKNHLEKRSDYDLKVNIFSNGENC
                     LDKMHEQPHVVILDYYLDGIKTDAANGLEILKKIRLQYPDTYVIMMSGQDDLKVAVAT
                     IDNGAYDYIIKGESAYVRAQMIIDHIIENINTEEWRKNVKVRENILVTGFILLGLVLI
                     SIALYDMGVF"
     gene            complement(12157..14175)
                     /locus_tag="PLL28_06845"
     CDS             complement(12157..14175)
                     /locus_tag="PLL28_06845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF69077.1"
                     /translation="MSEQQFPELLRELLSKYYLHSKSIERELYKSELKFRSLLEQLTD
                     AIYFTSVDGKFIEFNRATVDLLGYSSDELRELNVEATYVNPEDRDKFKAIVTANGEVI
                     DYELKLKSKSGDIIDCLVTSTVRYDSKGVILGYQGIIRDITLRKKAESLQRAKDLAEQ
                     ANKFKAEFLANMSHEIRTPINAIIGMVHLLHQTPLSSKQQEYLQAIDSSADNLLQLIN
                     DILDFSKIEAGKLQLENKSFSIRKVIEQLVNTMRFKADEKAIELKLNIDENVPESVAG
                     DPLRLNQIMLNLISNAIKFTHAGNITVTVRLIDTINDKARMYFNVRDTGIGIAPDKLN
                     HIFESFTQANMDTTRIYGGTGLGLSIVKKLIDMLNGAIMVKSKPGNGSEFIFEIDFTI
                     TRDAIELDQASSNDSGIPEIGACRMLLADDYPINQLVTTELLKHTWREMIIDVANNGK
                     EVLELLSKNDYELILMDVQMPEMNGLDATREIRKLQGPKAQIPIVAFTAYATTGEVDK
                     CLEAGMDDYISKPVDPAMLCRKIVEQLVKRNYQFPDPSAPKLAIEGVSPDLSYLDQMT
                     GEDSELKFKMINIMLEETPTELELLDSYCKAGNWDGVRAVAHKMKSSLQYMGLKETLE
                     LAKSIELAAREKVDVDQLPERINNVILTCKAALTSLSAELEKMQENEG"
     gene            14259..15230
                     /locus_tag="PLL28_06850"
     CDS             14259..15230
                     /locus_tag="PLL28_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNF69078.1"
                     /translation="MHSIAELSTLIEEGIFNAGFPQQPTGLYDPARYILSVGGKRIRP
                     LLALMGAQVFADDVKPHLPVALAMEVFHNFTLVHDDIMDNAPLRRGKQTVHEKWDVHT
                     AILSGDAMMIQSYVLLAGGPSNALMPLIDMFNKTALAVCEGQQDDMDFEKSEEVEIDA
                     YLEMIAKKTSVLLGCSLYCGAISAGANEDQAALLYEAGLKLGISFQIQDDILDTYGDS
                     SFGKQTGGDIARNKKTILFLMARDLANETDCALLLRLYHDSASSESEKIAAVRNIFDK
                     YAVKEHAESLMARYYEESRAALSSLHSTKNGVDPLLKLFDQVFTRTT"
     gene            complement(15232..15861)
                     /locus_tag="PLL28_06855"
     CDS             complement(15232..15861)
                     /locus_tag="PLL28_06855"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNF69079.1"
                     /translation="MIREFAQKLQKSLEFGLPGAPAHSIMAPLTRNVEPVEIGKLPNA
                     KKAAVLILFTPLDDKPAITLIERPAYEGVHGGQMSFPGGRIEESDVDVRYTALRETNE
                     ELGIDTSKIVTIGKLSDVYIPPSNYYVYPYLGYLPYAPEYFPDAREVKSVVTMPVSIL
                     FDPLSKGKMLIERPGISFEAPCYVYQQFRIWGATAIMLSELEWLLTRQV"
     gene            15935..>16759
                     /locus_tag="PLL28_06860"
     CDS             15935..>16759
                     /locus_tag="PLL28_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69080.1"
                     /translation="MKRRHTLLLLLLSPFAAFGQHHAKTGDLQFIENVGQWESPVEYV
                     AGVYGGELFLEKNAFTYHFSNAAELGHFKHDPNAGDPSSFVIKNHAFKVHFLHSDPLV
                     HLQGESIFDNYYNYFLGNDQSKWKGKVSAFGAVRYANLYPGIDMQVYSQSQSMKYDYI
                     IQPGISPAAIQIGYEGLDAIQLNDGNLELKTSINTVVEVKPLAYQIIQDKKVIVPCSF
                     VLNGNVVSYSFPEGYNTEYSLIIDPATLIFASYTGSTADNWGYTATYDNDGNLYGGG"
BASE COUNT         4826 a         3330 c         3488 g         5115 t
ORIGIN      
        1 tgtgaatggc gagaaagcat atgtagcacc tgagcagcaa aaatcatatg atgtcacatt
       61 taaagcaggt ttgggtgttg atgccgcagc cggtatttta ttgaagatga aactcaattc
      121 aatggatttc gaagataccg ttaacgtgac acctaataag attatgatca ctgcgaatat
      181 gagtattgcg gattcgaaac ttcttgcgaa aaataaacaa gtagaaagtg tgattttaaa
      241 tcacttcgac aggatggtag caggatatat tgggttattc ccgaataatc caaaccaacc
      301 gttaaattcc gttgatgatt tcagtccgat ttacataccc aaaaaaggag ctactgtgaa
      361 aattgatgcg aacagttatt ataattataa gcgcatcatt gaagcatatg agcacaatac
      421 agttacgctt gataaacaag gcagacctta catcgatggt aaattgctaa cagaatatac
      481 ctttaaacaa aattattact ggatgatggg tgataaccga catcgcagtt atgattcacg
      541 ttattgggga tatgttcctg aagaccatat tgttggcaag ccggttttac tttggttcag
      601 ctgggataag acattgccat tgagtgagcg gatcgccaat atccgctggg atagaatgat
      661 gcattttaaa tgatgctatg caaataaaaa taggagcgct attagccatt tcaatcctca
      721 tgctgcacgc atgtaaggag tctgccgaca atgaagacac agcggcaata gaaaatgttg
      781 gcgaaaaggg aggcgataat tcactacagg aattaataga taaagaagct actacacctg
      841 atgttgtgaa acaagcaagt ggcgatcagg tattgtataa cgaacgattc gattatacca
      901 ttaccattcc cgggttttac actattaaag aaaaaagcaa taatggtgat ggctatttta
      961 tttcctccgg cgacaaggat gtagatattc gcgtttacgg tgaaaacata gccgataatc
     1021 aggtgatggc agaaatggag ttaaatactt gtgaatccaa gacctcattt caattttcga
     1081 atggttatcc gggtattttg tgtatgcagt cagacgacag atattattac tacgacacac
     1141 caaaaacaag ggtcaccgtt tatgtgcatg cccctacccg atggttcgac aggaacaata
     1201 agctgctaga catcatggtt aagagtttgg tggttggaaa aggtgggttc tgatctatac
     1261 tatatcgcct atttgtcctt ctcaacacaa aggatttttg tcgtaaatcc aaagataatt
     1321 tatcagcacc cacgcttagc actttgcttc aataaccgta tttttatatc cgcatgcaga
     1381 ttatttttat cagtgttgga aagacttctg aaaagtgggt ttcagatgca tgttcaacct
     1441 atgtaaagcg attgggtaaa tatgtgagat ttcattggca tgaaacaaca gacattaaat
     1501 cacttccacc tgaggagatg aagctaagag agtttgcggc tattaaaaaa tatatagaac
     1561 aaggtgacca tgtagtgttg ttggatgaaa gaggtgctgc atacagttct gttggtttca
     1621 gtgaatttat tacacgcaaa cagctggaaa atcgcaaacg cttagtgttt gtgcttggcg
     1681 gcgcgcatgg ctttcatgaa gatgcctatt caatggcaaa tgaaacactt cgcttatcag
     1741 atatgacatt tcctcatcaa atggtacgca ttatttttct tgagcaatta tatcgtgcat
     1801 ttaccatcat caacagagaa ccctatcatc attaaaaaat ggaaaatgta acggtaacgg
     1861 ctttcattat cttattaact gcaataagca gtattctggc atttaacaat tctgtcatgc
     1921 gcggctcggc gttgttttat ccgcatgcta tgcatcaacg aggagaatgg tataggttta
     1981 tcaccagtgg ttttattcat gccgattata ttcatctgat tgtaaatatg tttgtgttat
     2041 ggtctttcgg tagtattctg gaagagcgtt tattaccgct agtttttcaa ggatatggac
     2101 gaatagttta cgttggtata tatttattgg ggatgatagt atctgatgtg ccttcctatt
     2161 tcaaacatcg caccgatgca acatatcgcg gacttggggc ttcaggcgct gtggcggcag
     2221 ttgtttttgc aagtattttg atcagtcctt ttgatggtga gatcggcttt tttctaattc
     2281 ccgggttgca tgttaaacct tttgtttttg gaattatata cttgatctac tctgcgtaca
     2341 tgggtcgacg tggtggtgat aatgtaaatc atgatgcgca tttttatggt gccttatttg
     2401 gattgatatt ccctggtcta ataaaaccgg aactttttat ttctttatat catcaaatct
     2461 tacaacaatg gacataattg atttacgcag cgacacagta acgaaaccaa gtgttgaaat
     2521 gcgcgctgct atggcatctg cacaagttgg cgatgatgta tttatggaag atccaagtat
     2581 tctggagctg gaacaaaaga tctgtgcgat gttcggaaca gaaatggcgt tgctcacacc
     2641 aacagctact atgtcgaatc aaatagcttt aaaaatatta actcatccac tggatgaaat
     2701 tatttgtgat agaacagcac atatatatag ctatgaagtt ggcggctatg cttttcattc
     2761 aggatgttcc attcgctatg tagaaggcga ccgcggtgtt tttagtgcaa cagatgtcat
     2821 caaacaaatt aatcctgatg atgtgcataa gccgataacg cgagtggtga gtattgaaaa
     2881 tacagtaaat cgcggcggtg gtcgcatcta ccctattgaa acaatcgctg aaattgctgc
     2941 ggtatgtcga gaaaagggat tgtcatttca tttagatggt tcgcgattat ttaatgcgct
     3001 tgttgcaagt ggtgctgatg caaagcaatt tggagaatta ttcgatacca ttacattgtg
     3061 tttcagcaaa gggttgggat gtcctgcagg gtctatttta acgggcaagg caatacatct
     3121 gcagaaagca cgcagggtga gaaaagtgat gggaggcggg atgcgtcagg ccggagtttt
     3181 agctgctgcc ggattgtatg cgttagagca caatatcagt cgacttgcag aagatcatca
     3241 acgtgcggcc gaaacaggaa atgcattaaa caaactgagt tatgtggaat cggttttacc
     3301 ggtagaaacg aatattgtta tcttcagact acgtgaaaat atatcagatg aaaaattcat
     3361 ccaatactta aatcatctag gaattaaagc atttgccata ggagacaatt gggtgcgttt
     3421 tgttttccac cttgatataa tatcaggcat ggaagaaaaa atagcatcag ccttaataaa
     3481 tttcaatgct taaccaatca ggttttgcag aatactgtta atggtttttg tgctgctttt
     3541 gtttccacca aacaaattaa aaataccttt caacatacca ctattgccat ttaatccgcg
     3601 tatggcatcg ttaatgtcaa atccaccggt ttgtgcatgc ttaactttac cattgagcaa
     3661 gttcataacc acaggtaagg cgattacggc taattgtttt gcagtttgat cagaaatatt
     3721 caacttactc tgcaattggt taatcacagg attctccagt tggcgaataa cttcatgatc
     3781 ttttgcagtt tccgaacctg acagcatttc tcttagtgca tcaacatttc cttttctggc
     3841 ttgctgcacg atggtattta caacggtttc ggaggtaaca cttgttacct gattactatc
     3901 caggtcgttc aattccggaa tgttgtggga gatcccatgc aggtgatctt cggctaggtt
     3961 cagtaattgg gcgatcatga tgttcgggtt taaggtgtat gtaaatctaa aaaaacgcct
     4021 tggttattcc aaattttaca ttatttcttc accattcgtt cctcaaagta aaaatgtgtg
     4081 gtatcgagct taaatgcctt aatatttggc aggtcaagcg aggtttcctg ccagtcggtg
     4141 gtaggtttca gccatttcca tgctccatcg gcacccatat atcgcaacgg catatcgaat
     4201 tcaggcacgt cggccgacca gcggtattcc aatttcaccg caccggcctc gggcgtcagt
     4261 ttgtattcaa atttcggcag gtctttatgt cttaaatatt ggtcaaattc ttttgagaaa
     4321 tccttccctg cttttctcga aatatacctt tcaatttgct cggtagtaac agtttgttgg
     4381 gcaaaatcct tttggatacc cttgatgata ctccaccaca gttggtcgtt gttcgtaagc
     4441 gtacgcaacg agttcaggaa tatggcacct ttgtgataca tgtcgccact gccttcgtta
     4501 ttgacattgt aatccccgat gatcggttca tcatttctta ctttttgctt catggcattg
     4561 atataattca tggcggtagc atttccatat aatgcctcca catagattgc ctcggaatac
     4621 gtgccgaagc cttcatgtat ccacatatca gccagatctt tcatgctcac attgttcccc
     4681 caccattcat gcgcagtttc atgaataatg atgtaatcaa aatccaggcc tatccctgag
     4741 taatcgaagc ctgaatagcc tgtttgatat ccgtttccat acgcgatggc actttggtgt
     4801 tccataccta catatggcgt ttcaacaagg gcatacccgt ctcgaggaaa aggataggcg
     4861 cccagatatt gctcatatgc tttcagcatt ggctttactt gctcaaattg cttttttgcc
     4921 ttgtcaacat tgtattccag cacataataa tcaagcgaat atgtagatcc atcaacattg
     4981 gtgtatgtat cttcaaaatg aacatacttg ccgatattca tggtaacatt ataattattg
     5041 attggatagg taaccgccca atgccatgtt ttccatcctt tttccggtat tgaagttgaa
     5101 atcaggttac cattagcaac tgccatcagt gtatcaggtg cagtgattgc aatgcgcata
     5161 ctttcaggtt catcactcaa atgatcttta ttcggccacc agacactggc tccgagccct
     5221 tgacatgaaa caccaaccca tggattcccc ttttcgtcgg tattccagct tattccacca
     5281 tcccatggag ccatttttgc aattctgggg tgaccgctgt aataaacagt gaattctcct
     5341 tgagtacctc ccttgattac ctttggaaag tcgacccaaa cagctgcaaa ttcacgttta
     5401 aactgaatat ccttaccctc aaagatgatt ttagtgatgc tgagttcctc aaacaagtcg
     5461 atttgcagtc gctgaaagtc gctcactgcc ttaaagttga tggtatttga gccttcaata
     5521 gccttattag caatgtccac ttttacggcc agatcgtagg taagcacatc gtagcaagtt
     5581 ctttcggcac gcaggcttcc acgcaaggta tcagccctgg tataagcgtg ttcaggatcg
     5641 aatagctggg cttgggattg gagtccaaaa atgatcagaa atattgctgt aatggtagat
     5701 ttcatgtgct gtacttgttt cgaatcacaa taataacagg tttttaaggt caaatctcgt
     5761 gcccttaacg cgaattaaca gacatgcaat atgaaactta gtcaaaaagg cgctcaggca
     5821 ccatctaaat aaggtggtta caaacagata acaatagatt tacaatttcc tgttaccttt
     5881 accttttaac gttgtcttta ccaattgcta acacgtgttt aacattagaa actcctcctt
     5941 agcgctctta ttgcctattg cgataggttt tctggcatca ggctgcactt cgctcccggg
     6001 tgatggtggc acttcaacca tacatggaaa gatctatgta gagcgttaca atgaatttgg
     6061 taccttgtac caagattatt acggacctga cgaacgtgtc tacatcattt atggagataa
     6121 tatcggctat gatgatgaag taaaaaccag ctttgatggc acttataaat ttccgttttt
     6181 aaggaagggc acgtatacca tctatgccta tagcgattgc ctcacatgcg atgggaatac
     6241 agaagcgcgc tctgtgcaag tagtgatcac agaaaacaac caggatattg aagttgaaga
     6301 tattgtaatt gaagcccgtt aattgcaccg catagattcg atattacatc aaattcaccc
     6361 catcacactt ggtgagatgg acaaggtgga gttaatgaat cgaaaggata ctaagtattt
     6421 tttcgacagg aagaagctca tagagatatt agatctgctg cagcacgatt atcgtgtatt
     6481 agaaattggc ggacatcgca ttttcaatta tcgcaatatt tatttcgaca ctcctgaatt
     6541 cgagtgctat cacatgcatc ataacaagcg tgtgaacaga tataaaattc gcattcgtca
     6601 atatgttgaa agcaatctga atttcctgga gatcaaattc aagattaata ccggtcggac
     6661 gattaaatcg cgcatcaaga tcgctgaatt ttcgccaata ctgagtgagg aagctaaacg
     6721 atttatttca gagaagacaa atttcgatcc ggagcaattg cgcccgacct tagaaaatga
     6781 ttttcgtcgt ttcacactgg taagtaattc aatgcgtgaa agggccacta tcgataccga
     6841 ccttcggttc aaaacgccaa acggtcaaat gcaattgagc aatgtgtgta tagctgaatt
     6901 aaagctcgat tccagttcat caggttcgcc atttaaatta gccatgcgcg ataatttttg
     6961 tccggaggcc agaatcagca aatacagtgt aggcgctgct tttatgtata gcgacctgaa
     7021 gcagaataat ttcaaattca agaaactcca aattaaccgt atagaaaatg tttagcactt
     7081 taatccaggc agccgaggaa caaaccaagt tcctagacct gaaattcttt gatgatgatg
     7141 tttacgatct gatactaagg tcgttcttca attttgtgat tgtttttatt ttagtaaaat
     7201 tcacctatcg cagcgatcgt aagaataaga actatgcgtt ttcattctac atcttcagca
     7261 tgttggtttt tttcctatgt tatctgttgt cgggcattaa acttgaaatg ggttttgcat
     7321 ttggattatt tgcaattttc tcaatcctga ggtaccgcac catcagcatt ccgatgaagg
     7381 aaatgactta cctctttttg gtaattgctg ttgctgttat taatgcacta acaactaaga
     7441 aggtatcctt cgtggaatta ttattcacca acgtttcgct gattgcagct acttatttcc
     7501 tcgaacgctt gtggtatcgt gaaggcttga gtgaacagat tgttgaatac gaaaagatcg
     7561 aaaatatcaa gcctgaacgc agagcagaaa tgcttgaaga cctgcgcatc agaaccggtc
     7621 tggatatacg ttcattcgaa atattaaata cagacttttt gcgcgacatg gcaaaggttc
     7681 gcatattctt taagcctaca cctcatttcg gcgaagaaga catggagcat gatgatgatg
     7741 acgattaata gagtcatatt aagcatttgc ttgctaacac tttcggtatg tgcaaaggct
     7801 caatataccg atggtggcgt ttggctttat agtggcatca atcacgaagt ggtaaaagat
     7861 ttaaaattag aattcggtga agagctgcga tataatatca gcactgacaa cccataccaa
     7921 tacaatactc acttttcggc tgcttataaa ctaaataaac attggaaaat tggtgccgac
     7981 tatcgctatt cagtccgtga aggattaaac accaatcgat atggcgctgc aataacctac
     8041 aagcagagta tacatgattt ggatatcagt ttgcgcagcc ggtttttgta tagtgtagtg
     8101 cttgatgggg ctgaaggtac agcctggcgt aataaacttg gatgtgatta taagattaat
     8161 aaagattttc aggttggcgt ttccggggag ttattttacg ctatgagcaa tgaattggat
     8221 caatttgata attatcgatt acaagccggc ctcacctact ctccgaacaa atatcatagt
     8281 ttggatgttg gatatattta tgatcatgaa ttcaatgtta acaatccggg gtcaatgcat
     8341 gtcgcgaata ttgggtacgt gtttagtttt tagcatttgt ctgtgtttat cgaaaaacac
     8401 taatgcacag attacaagct gcgatagcct gtcaactgat acactcagtg tgattgaaac
     8461 gcctaattca ttgatgatca acagcactat tttgtttcaa aagcttgccg ttccggaatt
     8521 atcactgcct gcttacaccc aaggtgtctt ttgcaatttt gaagacagga tcaacaaaaa
     8581 gacaccttta agaattgatt tcagcgtaaa gtgaaggtgc tgaaaatcac caccgatgta
     8641 tatcaattat aataaattta ttccatgcga aatgtagatc gatttgcaat aagtgattgg
     8701 ttcaaaggct tgcaagatca tatttgcgat gaaattgcca gcaccgatgg caagggtgta
     8761 ttcgagcagg agctatggca gcgcgaaggc ggcggtggtg gtagaacccg gatcatacaa
     8821 aatgcaaata ttcttgagaa aggcggcgta aattattctg aagtgcatgg tgtcttacct
     8881 tcccgtatcg ctgatgctat gcaggtttca ggtcatgatt ttttcgcaac aggcgtatcc
     8941 atcgtaatgc atccttttag tcccatggtg cctatcattc acatgaatgt gcggtatttt
     9001 gaagtgagca gtggtgagaa atggttcgga ggaggtattg atcttacacc tatttatatt
     9061 gacaagaccc aagctgcggt ttttcatcgc aaattgaaag aggtatgcga tcgtcatcat
     9121 gaaaattatt atgcggaatt taaacagtgg gctgatgatt atttctatat caaacataga
     9181 aaagaaacac gtgggatagg tggtattttc tttgatagat tacaggcaac agaatacatt
     9241 tcactttcgg acagatttga atttgtgaaa caagtcggga tgttgttttc agaaatatat
     9301 acgcgtctga tgcaggaaaa taaagatatt ccgtatggca aaccggaaaa gctttttcaa
     9361 gctttgcgac gcggcagata tgtggaattc aatcttgttt atgacaaggg cactaaattc
     9421 ggacttgaca ctgatggcag agcagaatct atattaatga gtcttcctcc tgaagccggt
     9481 tggccatata actataaacc aattgccggt agtcgggaag aacaaaccat agatctgctt
     9541 cgcaagggtg tggaatgggc ttcggcggat atttagaggc agtgtagaaa aattaattgt
     9601 gttgaatcag tgttactgat atgccctgcc ctatccacaa tgaatatttt gtaataaaag
     9661 gtatctatat tatctgaatc gtcgatacaa aacgaaattg gataatatgt ggtaattgtg
     9721 cctgaaattg ctttcgtatt accctggctt tgcacataag gtattttgaa cggtatttga
     9781 aagccgggag ccctgctgtc ttcccaaaac atatttacct cgtcttcact gccaatatca
     9841 ccgtctccat cttcaaaata tagtgtaatg ctcagactgt cctcatccag ctcatcgaaa
     9901 ttatcctgat taatacttac cacttcgatg taaggttcaa taggataaac gggaggcttt
     9961 acacatgctg cgaggagcat tgcgaacatt ataattagga agctgtattt cttcaccata
    10021 attttaccat tcaaaggtat atcctttata actcagaaac aatgcaagat attgcgcggt
    10081 tcacagaaag cattttcgaa gttacggctg atacatttgc cggcaaagcg cttgccattt
    10141 tcaactatca ggcgaaggaa aatgctgtgt accgtgattt tttacaatat ctcgacattt
    10201 ctccttcctc agtgaagtcg atttccgaca tccctttttt acctatttct gccttcaaac
    10261 accataaagt agtttgcgga ggccttgaaa cggataccta tttcgaaagt agtggaacca
    10321 ctgatagtca gcgcagcagg cattatatcg ttgacaggat gacgtatgaa aacagttatt
    10381 tgagcagttt caggcaagta tatggtgacc cgattaacta ccgcattttg gcactgctcc
    10441 cctcctacct ggagcgatca aatgcctcgt tggtggatat gtgtcggggg ttgatcgaac
    10501 ggtcgggcca tcctgagagt ggcttttatt tacatgatct gcaaagctta tcacaggttt
    10561 tgagcaatga tacaggaata aagaccatac tgattggtgt ttcctttgcc ttgcttgatc
    10621 tggcggagca atttcctcaa tccctcacaa ataccattat tatggaaacc ggaggcatga
    10681 aaggaagaag aagtgagatt acacgagagg ccttacatga tgttttgaga aatgctttta
    10741 atgtcgatgt ggtccattct gaatacggca tgacagaatt attttcacaa gcctattcaa
    10801 caggatacgg aattttccaa tgtccgccct ggatgcgcat aatcatacgc gacagtgagg
    10861 atccgtttca actgttggaa accggaaaaa caggtggcgt gaatatcatc gacctggcaa
    10921 atcttcattc ctgcagtttt attgccaccg atgacctcgg gtccatacaa caagataata
    10981 gattctctat tgccgggaga tttgacaaca gcgatattcg gggttgtaat ttaatgacct
    11041 tataagacta aggttacatt attttgaggc cgactgccct ttgtgtttca ttacgatatc
    11101 ccataagaca atacctgcac ttacagcaat attaagtgaa tgtttagttc cgaattgagg
    11161 aatctcgatg gatgcatcac acaatgccaa tgcatcttca cttacgccca tcacttcatg
    11221 tccaaaaacg aatgcaattt tttcgaatga ggaaggatca aaatcctgta aggctacact
    11281 gccggaagtc tgttctacag ctacgatgcc atatcccagt tccttcagtt ttttgatagc
    11341 atcaaccgtg tgatcaaaat attcccaata aacgctttca gtagcaccca aggctgtttt
    11401 ttcaatatcg cgatgcggag gagtggcagt aataccgcac aggcaaattc ccctaagcaa
    11461 aaatgcgtct gccgtgcgaa atactgatcc cacattattt tgacttcgga cattgtccaa
    11521 aaccacaaca aaggggtgtt tatcggcatt tttatattgt tctaccgata atctggataa
    11581 agctgcgttt ggcgtctttt gcatacgagg gctgcaaagt aacggaattg ggctaactta
    11641 tggtatttga attgtcagaa tacgcccata tcatacaaag cgatgctgat caacaccaat
    11701 ccgagcagga tgaaaccggt aacgaggatg ttttcacgca cttttacatt tttgcgccac
    11761 tcctcggtat taatattttc gataatatga tctatgatca tttgagcacg cacataggca
    11821 ctttcgcctt tgataatata atcatatgca ccattgtcaa ttgttgcaac tgctactttc
    11881 aaatcgtcct gcccgctcat catgattaca taagtatcgg gatattgcag tcggattttt
    11941 ttcagaattt ccagaccatt ggcagcatcc gtttttatgc catcgagata ataatccaga
    12001 ataaccacat gcggttgttc atgcatttta tcgaggcagt tttcaccatt tgaaaatata
    12061 ttcaccttca ggtcataatc agaccttttt tcgagatggt ttttcaacat aatcaggtgc
    12121 ttggtatcgt catctaccag aaacacctta aattgcttat ccttcatttt cttgcatttt
    12181 ttcgagttca gccgaaagac tggtcaatgc ggctttacat gttaaaatta cattattaat
    12241 tcgttccggc aactgatcta catcaacctt ttcgcgggcg gcaagttcga tggatttggc
    12301 cagctccagg gtttccttga gtcccatata ttggagcgaa gacttcattt tatgcgctac
    12361 agccctgaca ccatcccagt ttccggcttt acaataagaa tccagcagct cgagttctgt
    12421 gggtgtctct tctagcatga tgttgatcat cttgaattta agttcggagt cctcccctgt
    12481 catttgatcc aggtaactca aatcaggcga aacaccttca atggccagtt taggtgctga
    12541 tggatcggga aattgataat tgcgttttac caattgttcc acaatctttc tgcaaagcat
    12601 ggccggatcc acaggtttcg aaatataatc atccattcct gcctccaggc atttatcaac
    12661 ttcccctgtt gtagcatagg cagtaaatgc cacaattgga atttgtgcct ttggtccctg
    12721 taattttcgt atttctcgtg tagcgtcgag gccattcatt tcaggcatct gcacatccat
    12781 caaaatgagt tcatagtcat ttttcgaaag caattccaat acttctttgc cattgttcgc
    12841 cacatcgatg atcatttcac gccaggtatg cttcaataat tctgttgtaa ccaattgatt
    12901 gattggataa tcatctgcta atagcattcg gcaggcacca atttcaggaa tgccactatc
    12961 gtttgaagat gcttgatcta attcaattgc atcgcgtgta atggtgaagt caatttcaaa
    13021 aataaattca ctgccattgc cgggtttgct tttaaccatg atagcgccat taagcatatc
    13081 gatgagtttc tttacgatag ataatcccaa tccggtgcct ccataaatgc gggtagtatc
    13141 catgttggcc tgtgtaaaag attcgaagat atgattcagc ttgtcagggg caattccaat
    13201 tcccgtatca cgcacattaa aatacatgcg agctttatca ttgattgtat caattaatcg
    13261 aacggtcaca gtgatatttc cagcatgggt aaatttaatg gcattgctga tgagattgag
    13321 catgatctga tttaaacgca aagggtcgcc ggcaacagat tcaggaacgt tttcatctat
    13381 atttaatttc agttcgatag ccttttcatc agccttgaat cgcatggtgt ttactaattg
    13441 ttcgataacc ttgcgaatgc tgaatgattt attctcaagc tgtaatttcc ctgcttcaat
    13501 tttagagaaa tctaaaatat cattaatgag ttgcaataag ttatctgctg aagaatcaat
    13561 tgcctgcaaa tattcttgtt gttttgaaga aagcggcgtt tgatgcaata aatgtaccat
    13621 accgatgatt gcgttgatag gcgttcgaat ctcatggctc atgtttgcca agaattccgc
    13681 cttgaattta tttgcctgtt ccgctaggtc tttggcacgt tgcagtgatt ccgctttttt
    13741 tcgcaatgta atatcgcgga taataccctg atagcctaaa ataacccctt tactatcata
    13801 gcgtacggta gatgttacca gacaatcgat gatatcgcca cttttacttt ttaacttcaa
    13861 ttcataatct atcacttcgc cattggcggt aactatcgct ttgaatttgt cgcgatcttc
    13921 agggttaaca taggttgctt caacatttaa ttcacgcaat tcatctgaac tgtatccgag
    13981 caagtcgacc gttgcccggt tgaactcaat aaatttccca tcaacgctcg taaaatatat
    14041 ggcatccgtt aattgctcga ggagagatct gaatttgagt tcacttttgt acaattccct
    14101 ttctatcgat ttactatgta aatagtattt cgatagaagt tcacgtaaga gttcgggaaa
    14161 ttgttgctca gacatccgct acaaattaac gcaattagct gcactttaaa gcaattttgg
    14221 aggtagctga gccgctttga ttgccttatc tttgcggcat gcacagcatt gctgaattgt
    14281 ctacgctaat tgaagagggt atttttaatg ccggattccc tcaacagccc accggtttgt
    14341 atgatcctgc aagatatata ttgtctgtag gcggcaagcg tatcaggccg cttctggcac
    14401 taatgggggc acaggttttc gcagatgatg taaaaccgca tcttccggtg gcattggcaa
    14461 tggaagtatt ccacaatttc accctagtgc acgacgatat tatggacaat gcacctcttc
    14521 gtcggggaaa gcaaacagtg catgagaaat gggatgtcca tactgctatc ttatcaggtg
    14581 atgccatgat gatacaatca tacgtattgt tggcaggtgg cccgtcaaat gcgctaatgc
    14641 cattgatcga tatgtttaac aaaactgcac tggcggtttg tgaagggcag caagatgata
    14701 tggattttga aaaaagtgaa gaggtagaaa tcgacgccta tcttgaaatg atagcaaaaa
    14761 agacttctgt attattgggt tgcagccttt attgcggtgc catttctgcc ggcgcaaatg
    14821 aggatcaggc tgcattgctt tatgaagcag ggttaaagct gggcatcagt tttcagattc
    14881 aggatgatat tttagacact tatggcgaca gtagttttgg caaacaaaca ggtggcgata
    14941 ttgcccgcaa taagaaaacc attttgtttt taatggcgcg tgatctggcc aatgaaactg
    15001 actgcgcatt gctgttgcgt ttatatcatg attctgctag ttcggaatcc gaaaaaatag
    15061 ctgctgttcg caacattttc gataagtatg ccgtgaaaga acatgccgaa tccttgatgg
    15121 caaggtatta tgaggaatcg agggctgcat tgtcaagcct tcattcgaca aagaatggag
    15181 ttgatcctct tttgaagtta tttgatcagg ttttcacgcg caccacctga attaaacctg
    15241 tcttgtcagc aaccattcca attcactgag catgatagct gttgcgcccc aaatgcgaaa
    15301 ttgctgatac acataacatg gtgcttcaaa ggaaatacct ggacgttcga taagcatttt
    15361 cccttttgat agcgggtcaa acaatatcga aaccggcatg gttactactg atttcacctc
    15421 tctggcatca ggaaagtatt cgggagcata aggcagataa cccaggtagg gataaacata
    15481 gtaattactg ggaggtatat aaacatcact gagtttacct atagtaacaa ttttgcttgt
    15541 atcgataccc aattcttcat tcgtttccct caaagcagta taacgaacat ccacgtcgct
    15601 ttcttcaatt cgacctccag gaaagctcat ttgaccgcca tgaacgcctt catacgccgg
    15661 ccgttcgatc aatgtgatgg cgggcttgtc atctaagggg gtaaaaagta ttaaaacggc
    15721 tgcttttttc gcattcggta atttgcctat ctctacaggt tcgacattcc tggtaagcgg
    15781 cgccatgata ctgtgtgccg gggcgccggg caggccgaat tcaagactct tttgaagttt
    15841 ttgcgcgaat tcccgtatca tatttaggtc atttgacatg caacctcaca aagatgattt
    15901 atttaccgta aattagtagc gtgaaatatt gtgcatgaaa agaagacata ctctcctcct
    15961 actactgcta tcgccatttg cggcttttgg tcaacatcat gcaaagaccg gcgatttgca
    16021 atttatagag aacgtcggcc aatgggaatc gcctgttgaa tacgttgccg gtgtgtatgg
    16081 cggtgaactt tttcttgaaa aaaacgcctt tacttaccat ttttcaaacg ctgctgaatt
    16141 aggacatttt aaacacgacc caaatgcagg cgacccttca tcatttgtta ttaaaaatca
    16201 tgctttcaaa gtacactttt tacattccga tcctttagtg cacttgcagg gcgaatccat
    16261 tttcgataat tattataatt attttcttgg caatgaccaa tcaaaatgga aaggaaaagt
    16321 atctgcattt ggagcagtga gatatgcaaa tttatatccg ggaatcgata tgcaggtata
    16381 tagtcaaagc cagtccatga aatatgatta tatcatacag ccgggcatct cacctgctgc
    16441 aattcaaatt ggttacgaag gattagatgc tattcaatta aatgatggca atcttgaatt
    16501 aaaaacaagc attaataccg ttgtagaagt aaaaccgctt gcctatcaaa tcattcaaga
    16561 taaaaaagtt attgtgcctt gctcatttgt attaaatggt aacgttgtca gctactcatt
    16621 ccctgaagga tataatactg aatattcttt aatcatagat cccgctacct tgatttttgc
    16681 ttcatataca ggaagcactg ccgataactg gggatataca gccacatatg ataacgatgg
    16741 aaacctgtat ggcggagga
//
LOCUS       DAOQHY010000063        16632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1626_length_16632_cov_5.911806, whole genome shotgun sequence.
ACCESSION   DAOQHY010000063 DAOQHY010000000
VERSION     DAOQHY010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16632
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1626_length_16632_cov_5.911806"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1956)
                     /locus_tag="PLL28_06865"
     CDS             complement(<1..1956)
                     /locus_tag="PLL28_06865"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HNF69081.1"
                     /translation="MANEEKKLYLIDAFALIFRAYFAFARNPLINSKGQNVSAISGFV
                     NTLYDLLNNTNSTHIAVCFDSPEPSQREEMYADYKANREEAPEDIKFAVPYIKEIVKA
                     FHIPIIEKGGYEADDIIGTLAKKAKQAGYEVYMVTMDKDYGQLMEEGIFMYRPSYTGK
                     GFDTLTVDDILQKWEIERPEQVIDILGLMGDAVDNIPGVPGVGEKTAKKLIQEFGTVE
                     GLYENTDKLKGKLQENVVNNKEQALMSKVLATIMLDVPIEFEEDKLIIEEPDKEKLAA
                     IFQELEFRQLGKRIIGDSYSANVEKKTEQLDLFGNPTTTTSSQNAIRESREAVTAGKD
                     ISNVQHTYILADTPEKRKHLIQQLEKQTIFCFDTETTGIDANVAELVGLAFSWQQHQG
                     YYVPVPADKKEADKIIAEFKPVLESAKHTKVAQNLKYDYLALKWHGVDVVGPFEDSMI
                     AHYLLEPELRHNMDYLAENYLGYTPVSIETLIGKKGKNQLSMRDAPIDKVAEYSAEDA
                     DITLQLHQVFAPMLKKEELDTLYRTVEMPLIPVLADMEYEGISLDVDFLKEYSTLLEK
                     DMLELEKKIYEHAGVRFNIDSPKQLGDVLFIKMKIPYEGQKTKTGQYSTGEEVLAKLA
                     HAHPLAGLILDYRELGKLKSTYVDSLPL"
     gene            complement(2335..2679)
                     /locus_tag="PLL28_06870"
     CDS             complement(2335..2679)
                     /locus_tag="PLL28_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNF69082.1"
                     /translation="MSDQVTAFNEYRSKMNEVILSKQNKVINRLFNLDTNTYAEGALD
                     VKTKEMLGLVASMVLRCDDCIKYHLGKCHEQGITTDQMYEIFAVANIVGGTIVIPHTR
                     RAAEYWEELLKV"
     gene            2810..3436
                     /locus_tag="PLL28_06875"
     CDS             2810..3436
                     /locus_tag="PLL28_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNF69083.1"
                     /translation="MTTTTPAAAARNPKMGRYIGIGIVVVLILYLISLYNGIMNADET
                     VNQKWGDVESNYKRRYDLIDNLVNTVKGAADFEKSTLTELVEARAKATSITIDPATAT
                     PEQIKAFMAAQSGVSTSLGRLLAVVENYPQLTATQAYRDLMVSLEGTENRINVARMDY
                     NEAVKNYNVRIRKFPGNLIAGIAGLDRRNTFDAPEGVEDVPKVDFGTK"
     gene            3442..3885
                     /locus_tag="PLL28_06880"
     CDS             3442..3885
                     /locus_tag="PLL28_06880"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNF69084.1"
                     /translation="MPKARDFFTAEQQQQIVHAIKQAELKTSGEIRVHLEEKSGGDAY
                     GRAVKLFSSLKMDKIELHNGVLIYLAVVDRQFAIIGDSGIHAKVRQEFWDSMKEAMVS
                     HFRNEDFTGGLIAAITSAGEQLQQYFPRSSDDKNEMTDEISFKDA"
     gene            3878..4642
                     /locus_tag="PLL28_06885"
     CDS             3878..4642
                     /locus_tag="PLL28_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNF69085.1"
                     /translation="MRKFLILYVSLLLAIPVFAQDDEDLPARPVPAHLVNDLGNVFAA
                     DQEMMLEQKLLAYNDSTSTQITVVTIASVQPYEIGDYAYKLGRKWGVGQADKDNGIVI
                     LVAVSDHKVFIATGYGIEGYLPDIATSHIINDNFLPAFRKNDYYGGINAGLDEMYKYL
                     TGAYEGNPYGSGSPSDIPWGTVIIIVIVILFIISSRNKRNGGTTFTGGGRRTWGYGGG
                     YFGGGGFGGGFGGGGGGGFGGFGGGGFGGGGAGGSW"
     gene            complement(4772..5143)
                     /locus_tag="PLL28_06890"
     CDS             complement(4772..5143)
                     /locus_tag="PLL28_06890"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNF69086.1"
                     /translation="MATDQAPFFDLVWQVVRLIPKGRVTSYGAIARYLGTGRSARMVG
                     WAMNASHHAKPKVPAHRVVNRNGLLSGKAHFDPPEKMEQLLEKEGIKVKNNQIIDFKK
                     HFWDPNTELEEPDLMARSGKD"
     gene            5179..5724
                     /locus_tag="PLL28_06895"
     CDS             5179..5724
                     /locus_tag="PLL28_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69087.1"
                     /translation="MLQVPVKAGSITHLTDARFFAAYDVPYIGFNFDPLSPNYISPTQ
                     AMAIKGWLTGPQFVAEFDNQDEENIRNIIAFFQPDIVQFTASIIRENPQLLQITELPL
                     IIDLNDACIPADSNNILFYIVDKLNRSHAIPAEKCMINYRDEIPPPNPQTPIHLTGTP
                     EQLTGIKVYDAIGDWMEGVSG"
     assembly_gap    5949..6048
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6079..6768)
                     /gene="trmB"
                     /locus_tag="PLL28_06900"
     CDS             complement(6079..6768)
                     /gene="trmB"
                     /locus_tag="PLL28_06900"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNF69088.1"
                     /translation="MRKSKLARFAELDTFTNVLQNFNILEPVVVNNKKEVVDPKGKWH
                     DMIFRNQNPIVLELACGKGEYTCNMALADKSKNFIGVDIKGNRMHRGAKRALLEHIDN
                     AFFLRTDINIIETFIGPNEISEIWITFPDPHLKTGKFLKRLTSPRFLEKYKNILQPDG
                     IIHLKTDDPTLYEYTLEVIKEEGHRLIYADDDIYTKPLVNPFLEIKTYYEGMHLAEGR
                     KIKYVAFRLKA"
     gene            6850..8520
                     /locus_tag="PLL28_06905"
     CDS             6850..8520
                     /locus_tag="PLL28_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNF69089.1"
                     /translation="MKKLFRTKSISSVMANSDVADDIDHGSGLHRALTVRDLTFFGIA
                     AIIGAGSFSSMGSACAAGGPAVILLFIICGIACGFTAMCYAEFASRIPVAGSAYTYAY
                     TSFGEIIAWIIGWALLMEYSIGNIYVAFAWSDYFENLLGVIGIHMPGWLTTNYRSAHA
                     EMGDIWNNAPTLGSLHIIFDLPALLINFLITWLVFVGVKESRNVSNAMVIVKLAIIFL
                     VICVGAFYVNPDNWTPINDAGVRSFMPEGFTGVMSGVAAVFFAYIGFDAVSTMAEEAK
                     NPQRDLPRGMMYSLIICTIIYIILSLVLTGMAYYKDLKVGDPLAAVFQMNGVGWMEAI
                     ISIAAVAAMTSVILVFQMGQPRIWMSMSRDGLLPKKFQEIHPKYKTPGFSTIVTGLVV
                     GIPILFTDETFVLDFTSIATLFAFVLVCGGVLLIPRREKEKGRFHMPYVDGRYIYPIV
                     IFAAILFLNLIPATRGYFKDLFQIDFTADTATYKISSLIFWSLCVVLSVFAYKRRWSL
                     IPLLGLTTCCYLLTGMTAKNWVWFFIWFGLGLIVYFAYSRKHSKLNVA"
     gene            8606..10309
                     /locus_tag="PLL28_06910"
     CDS             8606..10309
                     /locus_tag="PLL28_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HNF69090.1"
                     /translation="MSQPAKGHPKGLYVLFFTEMWERFSYYGMRAIFLLYLTDALVYA
                     IDDASNIYGSYTGLVYLTPLLGGYISDKYWGNRRSIFTGGILMAIGQFLMFTSGSMLA
                     NPDTAKAIMLVALTFLIVGNGFFKPNISTMVGQLYPKGDSRVDSAFTIFYMGINLGAF
                     FSPLVCGGLGDTGNPGDFKWGFLAAGIGMIVSVIAFEFLKNKYLVTPDGRAVGMPPKY
                     YESAQPIATNASTNTLDNVDMENAASPKETPNFDGKMVGIAGVVAVGLFAVFYFLLGI
                     DLIGSCIFSVAIAIPYLIITDKSLNSHEKSRIWVIFILAFFVIFFWSAFEQAGASLTY
                     FADKQTDRNILGWEMPASWFQSINPLGIIILAPLFSMLWVALAKRKREPASPLKMAIG
                     LFLLCLGYIVIAFGVKGVDPSIKISMWFLVMLYLLHTMGELCLSPIGLSMVSKLAPLR
                     FASLLMGTWFLANAAANKLAGTLSALIPPGEEQFQKAQAAGIDLNSILNGTTAATADQ
                     AKVMTEQAIPTAWPSLMGMEITNLYQFFMVFIVMSGVAAFILFFLSGKLKTMMHTEDN
                     N"
     gene            10509..12218
                     /locus_tag="PLL28_06915"
     CDS             10509..12218
                     /locus_tag="PLL28_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HNF69091.1"
                     /translation="MNKGSKAKHPKGLYVLFFTEMWERFGYYLMIGIFALYMKDAWES
                     GGMGFSAEKKSDVYGTYIGLVYLTPFIGGLLADRLLGYRRSIFIGGSLMAAGYFGLSI
                     HDPNAFYISLLLIILGNGFFKPNISTLVGNLYNDDRYRANKDEGYNIFYMGINIGAFV
                     CNFVAAYLRINLGWGYAFIAAGIGMIIGLIIFASGTKHVKEADVLKPAKPGDMGTGVI
                     LASVLIPMFAFGVLGYIIPDNIFGTDTNDAFLFGCIPVILFFIYILFKSDKEDRKPVA
                     ALLSVYGCIVIFWAVFHQNGDALTVWAERYSDRELNGGAKSVAETMKIAQTVENHPSA
                     FYDDEKFKSYLDSLDARRIAFSKAGNMDSVKAISETITIRTGSHTYFANLPAEDLPQP
                     DSNLKLASTELFQSINPFWVVVLTPIVVGFFGFLRRRGKEPSTPTKIALGLLITGLSA
                     FIMVAAVKSANIESEKVSSMWLFATYGVITLGELFLSPMGLSLVSKLSPPRLTALMMG
                     GFFLSTSVGNKLAGILSSNFERAEDKSTFFLTNVFLALAAAAMLFALLRWLNAIMKEK
                     GLK"
     gene            12250..13836
                     /locus_tag="PLL28_06920"
     CDS             12250..13836
                     /locus_tag="PLL28_06920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HNF69092.1"
                     /translation="MSELKSTLDFTEEKDFNTSEEHKALDAIRSFKGNYPRQLWYLFF
                     SEMWERFSFYGMRGMLTFFMINQLFMDEKVANLQYGATQAFVYAFTFIGGMFADKILG
                     FRKSLFWGGILMITGSLILAINPQEFFFLGISFNIIGTGFFKPNISTMVGLLYKDGDS
                     RRDAGFSLFYSGINIGALLGGYACIAIGKGTMLGSVIPENLHWNVAFGLVAVVMTISL
                     LTFVRTRHSLGPIGLSPVNLRAQKGGLELSRRWPEYLVYVGALVCIPLIIVMVSTPRY
                     VDLFMNIIGPASLLYLGYEMTKHNKQENLKLLAALCFILFSVIFWAIYEQAGGSLSIF
                     ADNNLNNDFLGGLIQLDANGVNNAANALFVIIMAPLVGLLWVSMSKRKIEPNTVVKFG
                     ISFLFLGGAYFIYYSLRFFADDNGISSLDMFTLAYFVVTLGELCLSPIGLSIMTKLSP
                     TRLQGVMMGMWFLASAYGQYAAGLFGASIVADSPDATNTDKLISYTSGYYDFAIYAII
                     AGVVLLAISPLVRKLMGNVK"
     gene            13962..14765
                     /locus_tag="PLL28_06925"
     CDS             13962..14765
                     /locus_tag="PLL28_06925"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNF69093.1"
                     /translation="MEIIGYLGALLVGLTLGMFGAGGSILTLPVLVYLFGIDTSLATA
                     YSLFIVGATALAGAIPNMRRGTISYRTAIIFSIPSLTAVYLTRAYLMPAIPETLFSVN
                     DTVITKDIALLIFFALIMIFAAISMIRHRKIPDHSHDPDSAFNYLMILAEGSIVGVVT
                     GLVGAGGGFLIIPALVLFARLPMKMAVGTSLLIIAIKSLLGFVGDIQSGQPIDWPFML
                     IFTGVTIVGIFIGSALGRVFDAQKQKGAFGWFVFIMGVIILIEELILGL"
     gene            14794..15318
                     /locus_tag="PLL28_06930"
     CDS             14794..15318
                     /locus_tag="PLL28_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNF69094.1"
                     /translation="MTTRSIIIAAVLLLLIGFISVKRIRAQGHVDSTAYDVMLSTLLK
                     HNVPEININIAAADTNAIFLDAREHKEYAVSHIPEAIWIGYDDFSLSRVDSIDHSQPI
                     IIYCSVGYRSEVITGKLKKAGFTNVQNLYGGIFEWVNEGHQVVDDQGTETTRVHAYSK
                     KWGVWLNKGEKVYD"
     gene            15396..16274
                     /locus_tag="PLL28_06935"
     CDS             15396..16274
                     /locus_tag="PLL28_06935"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNF69095.1"
                     /translation="MSHYLDIFIDAYRGFAHYFWQSILHPSWNNFFYWLIGISIVVWA
                     LEVIFPWRKNQALIRQDFWLDAFYMFFNIFLFSLIGYYAISNVFVELFNDFLSLFGIK
                     NGIAIEINALPFWLKITFAFLLKDFIQWNVHRLLHSNKYLWQFHKVHHSVKQMGFAAH
                     LRYHWMENVIYKSIQYIPLAMLGFGISDYLIAGLITLVIGHLNHANINLRLGPLKYIL
                     NNPQMHIWHHAKHIPEQYPNGINFGISLSIWDYLFGTNYMPYSGRDIELGFAGDEHFP
                     HDFGHQAIYPAGKKQD"
     gene            16387..>16632
                     /gene="pyrH"
                     /locus_tag="PLL28_06940"
     CDS             16387..>16632
                     /gene="pyrH"
                     /locus_tag="PLL28_06940"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788558.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNF69096.1"
                     /translation="MNPPYKRILLKLSGEALMGGQQYGIDPVRLQQYAMEVKKAADAG
                     VQIAIVIGGGNIFRGMSGVGSGIDRAQGDYMGMLATVI"
BASE COUNT         4395 a         3267 c         3663 g         5207 t
ORIGIN      
        1 taatggcaat gaatccacgt atgtagattt caatttacct aattcacgat agtcgagaat
       61 gagtcctgca agcgggtgcg catgtgccag tttcgcaagc acttcttcgc cggtgctgta
      121 ttgtcctgtt tttgtttttt gtccctcgta aggaattttc attttaatga acaacacgtc
      181 accaagttgc tttggagagt caatattaaa gcgcacacct gcatgctcat agattttttt
      241 ctccagttcg agcatgtctt tttcaagcag ggtgctgtat tctttcagga agtcgacatc
      301 cagactgata ccttcatatt ccatatcggc taataccgga atgagcggca tttccacggt
      361 ccggtatagt gtatccagtt cttctttttt cagcataggc gcaaacactt gatgcagctg
      421 caaagtgata tcagcatctt cggctgaata ttcagctact ttatcaattg gtgcatcgcg
      481 catgctcagt tgattcttgc cttttttacc gatgagtgtt tcaatgctca ccggggtata
      541 tcccagataa ttctcggcga gataatccat attatgtcgc aactccggtt ctaataaata
      601 atgtgcgatc atactgtctt caaacggacc taccacatca acaccatgcc attttaaagc
      661 gaggtaatca tatttcaaat tttgtgcaac ttttgtatgc tttgcacttt ctaaaacagg
      721 tttaaattct gcaatgatct tatcagcttc ttttttgtcg gcaggaacag gcacataata
      781 tccttgatgt tgctgccatg aaaatgcaag cccgactaat tcggcaacat ttgcatcaat
      841 accggtagtt tcggtgtcga agcagaaaat ggtttgcttt tcgagttgtt gaataagatg
      901 cttacgcttc tccggcgtat cggcaagaat gtaagtatgt tgaacattcg aaatatcttt
      961 tccggccgta acagcttctc tgctttcgcg aatggcattt tgagaagatg ttgtagtagt
     1021 agggtttcca aagagatcaa gttgctcagt tttcttttca acgttggctg agtaactgtc
     1081 gccaataatg cgtttgccga gctgacggaa ttctaattct tgaaaaatag ctgcaagttt
     1141 ttctttatcc ggttcttcaa taatgagctt atcttcttca aattcgattg gcacatcgag
     1201 catgatggta gctaatactt tactcattaa tgcttgctct ttattattta ctacattttc
     1261 ctgcagtttg cctttcaatt tgtcggtatt ttcatataat ccttcaactg ttccgaattc
     1321 ctgaattaat tttttcgccg tcttttctcc aacaccggga acgccgggaa tattatccac
     1381 tgcatcgccc atcagtccga ggatatctat tacctgttcc gggcgttcaa tttcccattt
     1441 ctgtaaaata tcatcaacag taagcgtatc aaatcctttt ccggtataac tgggacgata
     1501 cataaaaatg ccttcttcca tcagctgacc ataatcttta tccatggtca ccatatatac
     1561 ttcgtatccg gcttgttttg ctttttttgc gagcgtgccg atgatatcat cagcttcata
     1621 tcctcctttt tctatgatgg gaatatgaaa tgcttttaca atttccttga tatatggcac
     1681 agcgaatttg atatcctccg gggcttcttc gcggtttgcc ttgtaatcgg catacatttc
     1741 ttcgcgttga gaaggctccg gtgaatcgaa gcatactgca atatgtgttg aattggtatt
     1801 attcagcaga tcgtataagg tatttacaaa tcctgagatt gcagaaacat tctggccttt
     1861 cgaattgatc agcggattgc gcgcaaacgc aaaataagcc ctgaatatca acgcaaatgc
     1921 gtcaatgaga tataattttt tttcttcgtt agccatgata ttaaaataag ggtttgcaag
     1981 atagggaaag gtggagaatt agccaattaa ctagtggaat gtcaggaggt ggtgtctttc
     2041 gagagtgtgc aagtgtgcaa gtctgcaaat gtgcaagtga aatgcaattg aggtgtgcgg
     2101 gtccagcttt ggctgggatg tcgctgcgct ccatgtggga atgggcaaac tgttgtgttc
     2161 gcgcgaaggt gctaaggggc aaagacgcga agtagtgata gtccgtttga ttctcagaag
     2221 ttaaatcaca gagtttgcgg gagttagcga aggagagtat gaacagagcc acaaacctaa
     2281 acactttcac accttcacat ggagcgcagc gacatcccgc cctcagcggg actttcacac
     2341 tttcagcagt tcttcccaat actccgctgc tctccttgtg tgcggtatca cgattgttcc
     2401 gcctacaata ttggcaacag cgaatatctc atacatctga tctgtggtga taccttgttc
     2461 gtggcatttg ccgaggtggt atttgataca gtcgtcgcaa cgcagcacca tactggctac
     2521 aaggccgagc atttctttgg ttttgacatc cagggcgcct tctgcatagg tattggtatc
     2581 gaggttgaag agtcggttga tcactttatt ttgcttggaa aggatcacct cgttcatttt
     2641 tgaacggtat tcgttgaaag cagttacttg atcgctcata gcagggtttt ggccaaagtt
     2701 cagtcacggg gcaggaaact ccaaacttta caatattttg gtcgtgattc cgtctttatg
     2761 gtctgattgg gtggtatacc ctatctttac ctgacaaaac aacctgaaaa tgacaacaac
     2821 aacacctgct gctgctgctc gtaacccgaa aatgggtcgc tatatcggta ttggtatagt
     2881 ggtagtgctc atcctttatc tgataagcct ctacaacggc atcatgaacg cagatgaaac
     2941 tgttaaccaa aaatggggtg atgtggaatc taactacaaa cgccgttatg acctcatcga
     3001 taacctggta aacaccgtaa aaggtgcggc agattttgaa aaatcaaccc ttaccgagtt
     3061 ggttgaggcg agagcaaaag ctacaagcat aaccatagat cctgcaacgg caacacctga
     3121 acagatcaaa gcatttatgg ctgcacaaag tggcgtatct acttccttgg gtcgcttact
     3181 cgctgtagta gaaaattatc ctcaacttac cgctactcag gcatatcgcg atctgatggt
     3241 gtcattggaa ggcacagaaa accgtatcaa tgtggcccgc atggattaca acgaagcggt
     3301 gaaaaattac aatgtgcgta ttcgtaaatt ccccggtaac ctaattgccg gtattgcagg
     3361 tctcgatcgt cgcaatacat ttgatgcacc ggaaggtgtt gaagatgtgc ctaaggttga
     3421 cttcggtaca aaataatcga catgccaaaa gcgcgtgatt ttttcactgc tgaacaacag
     3481 cagcagattg tgcatgctat caagcaggct gagttaaaaa cctcaggtga aatacgtgtg
     3541 catctggaag aaaaatcggg tggggatgct tatggtcgcg ctgtgaaatt gttttcttcg
     3601 ctgaaaatgg ataaaataga actccacaat ggcgtactga tttatcttgc tgtggtggat
     3661 agacaatttg ccatcatcgg agattcgggt atacatgcaa aagtgcgtca ggaattttgg
     3721 gatagcatga aggaggcgat ggtgtcgcat tttcgcaatg aagattttac aggtggtttg
     3781 attgctgcta tcacttcagc aggagaacaa ttacaacaat attttccacg atcatcggac
     3841 gataagaacg aaatgaccga tgagatatct ttcaaggatg cgtaaatttc tgatcttata
     3901 tgtaagcctg ttgcttgcaa ttcctgtgtt tgcgcaggac gatgaggatc tgcccgcgcg
     3961 tccggttccg gcgcatcttg tgaatgacct cggcaatgtt ttcgctgcag atcaggagat
     4021 gatgctggag caaaaattat tggcatataa cgattctact tcaacgcaga ttaccgttgt
     4081 cacaatagct tctgtgcaac catatgaaat tggtgattat gcttataaat taggaagaaa
     4141 atggggcgtt gggcaggctg ataaagataa tggtattgta attctagttg cggttagcga
     4201 tcataaagta ttcattgcaa caggttatgg tatcgaaggt tatttacctg atattgcaac
     4261 atcacatatc atcaacgata attttttacc tgcatttcgt aagaacgatt attacggcgg
     4321 aataaatgcc gggcttgatg aaatgtataa atacctgaca ggtgcctatg aagggaatcc
     4381 gtatggcagt ggatcgccat ctgatattcc ctggggaaca gtaatcatta ttgtcatcgt
     4441 aattcttttt attatttcca gtcgcaataa aagaaatggc ggaactacat tcaccggtgg
     4501 cggtcgcaga acatggggtt atggtggcgg atattttggc ggaggtggat ttggcggtgg
     4561 cttcggagga ggcggtggcg gcggctttgg cggcttcggt ggtggcggct tcggcggcgg
     4621 cggagcaggg ggatcgtggt aataagggta atcgtagttg tttcacgcag agacgcagag
     4681 tcgcagagtt ttaatgcatt tactgtaaca aagttacagt aatcaatagg ctagtctcac
     4741 tacccactac tcactaccta aagctcagat cttaatcctt accacttctt gccatcaaat
     4801 ccggctcctc aagctcggtg ttcggatccc agaagtgttt tttgaaatcg atgatctgat
     4861 tatttttcac cttgatacct tctttctcta ataactgttc cattttttca ggtggatcga
     4921 aatgggcttt gccgctcaat aatccattcc tatttaccac acgatgcgca ggaacctttg
     4981 gctttgcatg atgtgatgca ttcatggccc aaccaaccat acgggcgctg cgaccggttc
     5041 ctaggtagcg tgcaatggcg ccgtaactgg taactcttcc cttgggaatc aatcggacca
     5101 cttgccagac aaggtcgaaa aagggtgctt gatcagttgc cataaaaaat atgggttgaa
     5161 agtcggaatt ttgtggcaat gttacaagtt ccggttaaag caggcagtat cacacatctg
     5221 accgacgcgc gattttttgc ggcatatgac gtgccataca taggatttaa tttcgatcct
     5281 ttatcgccaa attatatttc tcccactcag gcaatggcaa taaaaggttg gctaaccggt
     5341 ccgcagtttg ttgctgaatt cgataatcag gatgaagaaa atattcgcaa tatcatcgca
     5401 ttttttcagc ccgatattgt tcaatttaca gcatcaataa ttcgtgaaaa tccgcaatta
     5461 ttgcagataa cagaattgcc attaattatc gatttgaacg atgcatgtat tcctgccgat
     5521 agtaataata ttctgtttta tatcgtcgat aaacttaatc gctctcatgc gattcctgca
     5581 gaaaaatgca tgatcaatta tcgcgatgaa attcctcccc caaatcccca aacccccatc
     5641 caccttaccg gaacccctga gcaacttacg ggcataaaag tatatgacgc aattggcgac
     5701 tggatggaag gggtgagtgg gtgagtatcg tgagtcgtga agcgtgaatc gtgagttttt
     5761 ttttcacgca gagtcgcaga gtcgcagagt tttaatgcat ttacggtaac gacgttacag
     5821 gcagtattgg gctagtacca ctactcacta ccaatcgtga atcgtgattc gtgactcgtg
     5881 aattgggaac ttgcctggtc gtgacacctc cgccaactcc gcgattaaaa tagggtgagt
     5941 gggtgagtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg atacctgata
     6061 ccggctccct gataccaatt aagctttcaa cctaaacgcc acatacttga tcttcctccc
     6121 ttctgcgagg tgcatgcctt catagtaggt ttttatttcg aggaatggat ttaccagtgg
     6181 cttagtataa atatcatcat cggcgtagat gaggcgatgt ccttcttcct taattacttc
     6241 aagcgtgtat tcgtataaag taggatcatc agttttgagg tggatgatgc cgtcgggttg
     6301 aaggatgttt ttatattttt caaggaaacg cggactggtc aggcgcttca gaaattttcc
     6361 tgttttcaga tgaggatcgg gaaaagtgat ccagatctcg ctgatttcgt ttggaccgat
     6421 gaaggtttca ataatattga tatctgttct cagaaaaaag gcattgtcga tatgctccaa
     6481 cagggctctt ttggctcctc tgtgcatgcg gttgccctta atatcgacac caatgaaatt
     6541 tttggattta tcggcaagtg ccatgttgca ggtatattca ccctttccgc acgccaactc
     6601 tagaactatc ggattttggt tgcgaaatat catgtcgtgc cacttccctt tagggtctac
     6661 aacctctttc ttattgttta ccacaacggg ttccaggata ttgaaattct gcaacacatt
     6721 ggtaaaagtg tccagttcgg caaacctggc aagcttactt tttctcacgc cgcaaaggta
     6781 agcaataggc ttctgccttg tttattcggc aaaatccctt tacttttcga cctcaagaac
     6841 cgctattgaa tgaaaaaact ctttcgaacc aagagcatca gcagtgtcat ggccaattcc
     6901 gatgtggctg atgacatcga tcatggctcg ggcttacaca gggcgctgac cgttcgcgat
     6961 ctcacatttt ttggtatcgc ggccatcatt ggagccggta gtttcagcag tatgggctcg
     7021 gcttgtgcgg caggaggacc ggctgttatc ctgcttttca ttatttgcgg gatagcctgc
     7081 gggtttactg ccatgtgcta tgcggaattt gccagtagaa ttccggtagc aggaagtgct
     7141 tatacctatg cctatacctc ctttggcgaa atcatcgcct ggatcatcgg ttgggctttg
     7201 ttgatggaat attccattgg taatatatat gtagcttttg catggagcga ctatttcgaa
     7261 aatttacttg gagtaatcgg tatccatatg cccggatggc tcactaccaa ttatcgctct
     7321 gcgcatgccg agatggggga tatctggaat aatgcaccta cccttgggtc tttacatatc
     7381 attttcgacc tccctgcgct gctcatcaac ttcctgatca cctggctggt gtttgtgggt
     7441 gttaaagaaa gtcgcaatgt cagcaatgcc atggttatcg ttaagctggc gattatattc
     7501 ctggtgattt gtgtcggtgc tttttatgtg aatccggata actggactcc cattaatgat
     7561 gccggtgtga gaagttttat gcctgaagga tttacaggag tgatgagtgg agtggcagca
     7621 gtattttttg cctatatcgg tttcgatgcg gtgagtacga tggcagaaga agccaaaaat
     7681 cctcaacgtg atctgccgcg tggaatgatg tattcattga ttatttgcac cattatttat
     7741 atcattctat cacttgtgct tacaggtatg gcttattata aagatcttaa agtaggcgat
     7801 ccgttggcag cagtatttca aatgaatggt gttggatgga tggaagcaat catttcaatc
     7861 gctgctgttg ctgccatgac tagtgttatt ctggtatttc aaatgggaca gccacgtatc
     7921 tggatgagca tgagtcgcga tggattattg ccaaagaaat ttcaagagat tcatcccaaa
     7981 tataaaacac ccggattctc tacaatcgta acaggacttg ttgttggaat tcctatctta
     8041 ttcaccgacg aaaccttcgt acttgatttt acaagtattg ccaccttgtt tgcctttgta
     8101 cttgtttgcg gtggtgtgct tctcatcccc cgaagggaaa aagaaaaagg cagattccac
     8161 atgccttatg ttgatggcag atatatttat ccaattgtga tatttgctgc tattttattt
     8221 ttgaatttaa ttcctgccac aagaggatat ttcaaagatc ttttccaaat cgattttacg
     8281 gcagatacgg ctacctataa aatcagctcc ttaatattct ggagtttgtg tgtagtgctc
     8341 tctgtatttg catacaaacg cagatggtcg ctgataccat tactcggact caccacatgt
     8401 tgttacctgc ttacaggtat gacagcaaaa aactgggtgt ggttctttat atggttcggc
     8461 ctgggattga ttgtatattt tgcatacagt cgcaagcata gtaaattgaa tgtagcataa
     8521 cgaaatcgaa ttaaacacat taaaagtatt tacttacttc tattcatttt taacatcata
     8581 aatattcatt acaaaacaac accgtatgtc acaacctgct aaaggccatc cgaagggact
     8641 atatgtactc ttcttcacgg agatgtggga gcgtttcagt tattatggaa tgcgcgcgat
     8701 cttcctgtta tatttaaccg atgcattggt ttatgcaatc gatgatgctt ccaatattta
     8761 tggtagctac accggtttag tttacctgac tccgcttttg gggggatata tttctgacaa
     8821 gtattggggt aatcgccgct cgatttttac cggtggtatc cttatggcta tcggtcaatt
     8881 tttaatgttt acaagcggaa gtatgcttgc caatcctgat accgccaagg ctatcatgtt
     8941 ggtggcactc accttcctca ttgttggaaa cggattcttc aaaccaaaca tttctacaat
     9001 ggttggtcag ttgtatccaa aaggtgattc acgtgtcgac agtgcattta caattttcta
     9061 catgggcatc aatctcggtg catttttctc accactggtt tgtggtggtt tgggggatac
     9121 cggaaacccg ggagatttta aatggggatt cctcgcagca ggtattggaa tgatagtatc
     9181 tgtaattgct ttcgaatttt tgaaaaataa atacctcgtt acacctgatg gaagagcagt
     9241 aggtatgccg ccaaaatatt atgaaagtgc acaaccgata gctactaatg catctaccaa
     9301 tacacttgat aatgtggata tggaaaatgc tgcatcgcca aaagaaacac caaatttcga
     9361 tggaaaaatg gttggtattg cgggtgtagt tgcagtagga ttattcgcag tattttattt
     9421 cttacttggc atcgacctga taggttcgtg tatattctca gttgccattg cgattccata
     9481 cttaatcatc accgataaat cattgaacag ccacgaaaaa tcacgtattt gggtaatatt
     9541 tattttggca ttctttgtga ttttcttttg gagtgcattt gaacaagccg gggcatcgct
     9601 tacctatttt gccgataagc aaaccgacag aaatatttta ggttgggaaa tgcctgcaag
     9661 ctggtttcag tcaataaacc cacttggtat tatcattctt gcccctcttt tcagcatgtt
     9721 gtgggtagca ctcgctaaac gcaaacgcga gcctgcatca ccattaaaaa tggcaattgg
     9781 tttattctta ttatgtcttg gatatattgt gattgcattt ggtgtgaaag gtgttgatcc
     9841 ttccattaaa atcagtatgt ggttcttggt aatgctttat ttattgcata ctatgggtga
     9901 attatgttta tcgcctattg gtttatcgat ggtgagtaaa ctggctccat tgcgctttgc
     9961 atctttattg atgggaacat ggtttcttgc aaacgcagca gcaaataaac ttgccggaac
    10021 attgagtgcg cttattcctc cgggagaaga acaattccaa aaagcacaag ctgccggtat
    10081 cgacctgaac agtatattaa acggaacaac tgccgctact gccgatcagg cgaaagtaat
    10141 gacagaacaa gctattccta ctgcatggcc aagtcttatg ggaatggaga tcaccaattt
    10201 ataccagttt ttcatggtct tcattgtaat gagtggcgtg gcagcattta tacttttctt
    10261 cctcagcggc aaattgaaaa caatgatgca tacagaggat aataactaat cctttttcat
    10321 attcttgtaa aaaaaagcag ttgcctgatt cggtagctgc ttttttattt catgtataat
    10381 cctgatatct gctacttcaa attattaagc tttgctaaat cggcacagtt ttggctttcc
    10441 ggaacttgag aggcgtttgg ctctatggaa atcatgttta tctttagaca cccaaaaaca
    10501 ggcaattaat gaacaaaggc agcaaagcaa agcacccgaa aggcctatat gtgctctttt
    10561 tcacagaaat gtgggagcga tttggctatt atctgatgat tggcatcttc gccctttaca
    10621 tgaaagatgc atgggaaagt ggaggaatgg gattcagcgc agaaaagaaa tctgatgtct
    10681 atggaaccta tatcggtctg gtgtatttaa caccgtttat cgggggctta cttgccgaca
    10741 gattactcgg ctaccgacga tcaattttta tcggaggttc attgatggca gcgggatatt
    10801 tcggattatc gattcacgat ccgaatgctt tctatatttc tttattgttg atcattttgg
    10861 gcaatggctt tttcaaacct aatatttcaa cgcttgtagg taatttatat aacgatgatc
    10921 gttatcgtgc caacaaagat gaaggttata atatttttta catgggcatc aatatcggtg
    10981 ccttcgtttg caatttcgtt gcagcctatt tgcgcataaa tctcggttgg ggatatgcat
    11041 ttattgctgc aggaatcggg atgatcatcg gtttgatcat attcgcttcc ggcactaaac
    11101 atgtaaaaga agccgatgtt ttgaaaccgg ctaaaccggg agatatggga acaggcgtca
    11161 ttcttgcctc cgttcttata cccatgttcg cattcggagt gctgggttac attattcctg
    11221 acaatatttt cggaaccgat accaatgatg cgtttttatt tggttgtata ccggtgattt
    11281 tattttttat ttatatcctg tttaaatcag ataaagaaga tcgcaaaccc gttgcagcgc
    11341 tgttgagtgt ttatggatgt attgtaattt tctgggctgt atttcatcaa aatggcgatg
    11401 cgctcactgt ttgggctgaa agatattcgg atcgcgaatt aaatggcggt gcgaagtcgg
    11461 ttgcagaaac catgaagatt gcacaaacgg tcgaaaatca tccttctgca ttttatgatg
    11521 acgagaaatt caaatcatat ctcgatagtc ttgacgccag gcgtattgca ttcagcaaag
    11581 caggaaatat ggattcggta aaagcaattt cggaaactat aactatcaga accggatcac
    11641 atacctattt tgcaaatctt cccgctgaag atctccctca acctgatagc aatttgaaac
    11701 ttgccagcac cgaattattt cagtcaatca atccattctg ggtagttgtg cttacgccga
    11761 ttgtcgttgg attttttgga ttccttcgac gcagagggaa agaaccttca acgcctacca
    11821 agatcgccct cggactcctg atcacaggct taagtgcatt tatcatggta gccgcggtaa
    11881 aatcggcaaa catagaatca gaaaaggtaa gcagtatgtg gttattcgct acttatgggg
    11941 taatcacctt gggagaatta tttttatcac caatgggact ttcgcttgtt tcaaaattat
    12001 caccgcctcg attaacggca ttgatgatgg ggggattttt cttgtcgaca tcagtaggaa
    12061 ataaactcgc cggaatattg tcgagcaact tcgaacgagc agaagataaa tccaccttct
    12121 tcctgacaaa cgtattttta gcattagcgg cagcagctat gttattcgca ttattgcgat
    12181 ggttaaatgc cattatgaaa gaaaagggtt tgaagtaacc acaattacaa tttgcattaa
    12241 aacagatata tgtcagaact taaatctaca ttagacttta ctgaggaaaa agattttaat
    12301 acctcagaag aacacaaggc cttagacgca atccgatcgt tcaaaggcaa ttaccctcgc
    12361 caactttggt atctgttctt cagtgaaatg tgggagcgct ttagtttcta tggtatgcgc
    12421 ggcatgctta cattcttcat gattaatcaa ctgttcatgg atgaaaaggt agccaactta
    12481 caatatggcg ctacgcaggc ttttgtttac gcttttacat ttatcggcgg tatgttcgca
    12541 gataaaattc ttggattcag aaagtcatta ttctggggcg gtattttgat gattacaggg
    12601 agtttgattc ttgccataaa tccgcaagag ttcttctttc tgggtatcag ttttaatata
    12661 ataggcaccg gattctttaa acctaatata tccaccatgg taggtttatt atacaaggac
    12721 ggtgatagta gaagagatgc aggcttctcc ttattttact caggaattaa tatcggtgcc
    12781 ttgcttggtg gctatgcctg tattgccatc ggaaagggaa ctatgctcgg cagtgtaatt
    12841 ccggaaaatc tgcattggaa tgtagctttt ggacttgttg cggttgtaat gactatcagc
    12901 ttgcttacat ttgtaagaac aagacattca cttggtccta tcggcctttc accggtgaat
    12961 cttcgtgcgc aaaaaggtgg cttggaatta tctcgtcgct ggcctgaata tttggtatat
    13021 gttggtgcat tggtttgtat tccactgatc attgtaatgg tgagtactcc tcgctatgta
    13081 gacctgttta tgaatatcat cggccctgcc agcttgcttt atctgggata tgaaatgaca
    13141 aaacataata agcaggagaa tttaaaatta ttggctgcat tgtgttttat tttattcagt
    13201 gtgatcttct gggctattta tgaacaggca ggtggttcgt tgagtatttt cgccgataat
    13261 aatttaaata atgattttct tggtggtctt attcagctgg atgcaaatgg tgtaaataat
    13321 gcagcaaatg cactgtttgt gattatcatg gctccgctag tcggattgct atgggtgtcc
    13381 atgtcaaaac gaaaaattga gcccaatacc gttgtgaaat tcgggatatc atttttattc
    13441 cttggaggcg cttattttat ttattattct ttacgcttct ttgcagatga caatggcatt
    13501 tcttcattgg atatgttcac ccttgcatat tttgttgtaa cacttggaga attatgtttg
    13561 tcgccaatag gtttatctat catgacaaaa ctttcaccaa cgcgattgca aggtgttatg
    13621 atgggtatgt ggtttttggc aagtgcctat ggtcaatatg ctgccggttt attcggagcg
    13681 agtatcgtgg cggattctcc ggacgcaaca aatacagata aactgatatc gtacacatca
    13741 ggatactatg attttgcaat ttatgcgatc attgccggag tggtgcttct agccatttca
    13801 ccattggtgc gcaagctgat gggaaatgtt aaataattga agatatattg cttttagcaa
    13861 agggctcagt tccgttcagg gattgagccc tttgttttcc accatcattt gtgctaacag
    13921 ttacattata aacttctgaa ttcgcctacc tttgctccgc aatggaaata ataggctatc
    13981 tcggagcatt gcttgtaggc ttaacactgg gtatgtttgg agccggagga agtattttaa
    14041 cacttcctgt tttagtttat ttatttggta ttgatacgtc tctcgctact gcatattcac
    14101 tgtttattgt gggtgctact gcattagccg gtgctattcc aaatatgcga cgaggaacaa
    14161 tatcctaccg cacggcaata atcttttcca ttccaagtct caccgcagtt tatctaacaa
    14221 gagcttattt aatgccggcg attccggaaa ctttattttc ggtaaatgat acggtcatca
    14281 ccaaagacat agccttactg atatttttcg cactgattat gatttttgct gctatcagta
    14341 tgatcagaca tcgcaaaata ccggatcatt ctcatgaccc tgattcagca tttaattatc
    14401 tgatgatttt ggccgaaggt tccattgtag gtgtggttac cggactggtc ggagcgggag
    14461 gcggatttct tatcattcct gcacttgtgt tgtttgcacg tcttccaatg aaaatggctg
    14521 taggtacttc attgcttatc attgcaatta agtcgctctt aggatttgta ggtgatattc
    14581 agagcggaca gcctatagat tggccattca tgctcatttt taccggtgtt accatcgttg
    14641 gaatattcat tggatctgca cttggcaggg tatttgacgc tcaaaagcaa aaaggggcct
    14701 tcggatggtt tgtgtttatc atgggtgtga ttattctgat agaagaatta attttaggtc
    14761 tctgatgtta ttattgtgtt tcgaaaacga cgtatgacaa cacgttcaat aatcattgct
    14821 gcagtactct tgctgctgat tggatttatt tccgtcaaac gtatacgtgc acaaggtcat
    14881 gttgatagca ctgcttatga tgtcatgcta tcgacactac tgaaacataa tgtccctgaa
    14941 atcaatatca atatcgcggc cgctgatacg aacgcaatat ttttagatgc tcgcgaacat
    15001 aaagaatacg ctgtaagtca cattcctgaa gccatttgga ttggctatga tgatttttct
    15061 ctcagtcggg tagatagcat agatcactcc caacccatta tcatttattg ctcagtagga
    15121 tataggagtg aggttatcac cggaaaattg aaaaaagcag gcttcacaaa cgttcaaaac
    15181 ctctacgggg gaattttcga atgggtgaac gaggggcatc aggttgttga cgatcaagga
    15241 acagaaacaa ccagagtaca cgcctattca aaaaaatggg gcgtatggct gaataaaggc
    15301 gaaaaggtat atgactgatt gatgtgatga ttgtcacccg gcattgcttg ccttgccatt
    15361 atctttggcg tgtttagtaa agaaatcagt cggacatgtc acattatttg gatatattta
    15421 ttgacgctta tcgaggattt gcacattatt tttggcaatc gatattacat ccttcgtgga
    15481 ataatttttt ttactggctt attggcataa gtatcgtcgt ttgggcatta gaggtcatat
    15541 tcccgtggcg aaaaaatcag gcattgattc gtcaagattt ttggctcgat gccttttata
    15601 tgttttttaa tatttttctg ttttcgctga tagggtatta tgccatatcc aatgtgtttg
    15661 tagaattatt caatgatttt ttatccctat ttggtattaa gaatggaatc gccattgaaa
    15721 taaatgcact tccgttttgg ttgaagatca ccttcgcatt tttattgaaa gattttattc
    15781 aatggaatgt gcataggctt ctgcacagca ataaatattt atggcaattt cataaagtgc
    15841 accacagcgt taagcaaatg ggattcgctg ctcatttgcg ttatcactgg atggaaaatg
    15901 tgatttacaa aagcatacaa tatattccgc tggctatgtt gggttttggc atttcagatt
    15961 atttgattgc aggattgatc acgcttgtga taggtcattt gaaccatgct aatataaact
    16021 tgaggctggg tccgcttaaa tacattttaa acaatcctca aatgcatatt tggcatcatg
    16081 caaagcatat tcctgagcaa tatcctaatg gaatcaattt cggcatcagc ctgagtatat
    16141 gggattattt attcggcacc aactatatgc catattccgg cagggacatc gaattaggat
    16201 tcgcgggcga tgaacatttt ccacatgatt ttggccatca ggctatctat cctgcaggga
    16261 aaaagcagga ttgacgaagc attattctag cgagtgtgcc attgaggtca ttttcccgga
    16321 tgagtattgc ttttaaatcg gctatttttt cgaacctctg cccatgaagt tttatctttg
    16381 tgcttgatga acccacctta caaacggata ttgcttaaat tatccggaga agccctgatg
    16441 ggcggtcagc agtatggtat cgaccctgtc cgccttcaac aatatgccat ggaagtgaaa
    16501 aaagccgcag atgccggtgt gcagatcgcc attgttattg gcggaggcaa cattttcagg
    16561 ggtatgagtg gggtaggatc gggtatcgac agagcacaag gcgattatat gggaatgctg
    16621 gcaactgtca tc
//
LOCUS       DAOQHY010000064        16518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1644_length_16518_cov_6.409403, whole genome shotgun sequence.
ACCESSION   DAOQHY010000064 DAOQHY010000000
VERSION     DAOQHY010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16518
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1644_length_16518_cov_6.409403"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLL28_06945"
     CDS             complement(<1..336)
                     /locus_tag="PLL28_06945"
                     /inference="COORDINATES: protein motif:HMM:NF015546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HNF69097.1"
                     /translation="MPIWQLDHRNIFPDPFDADPDGLLAIGGDLSSDRLLLAYRSGIF
                     PWFTLGHEPYWFAPDPRCILDFQNLHISGSMKQLLKKKAFDVTLDKNFESVIKSCAQI
                     ERKNDESTWI"
     gene            complement(342..620)
                     /locus_tag="PLL28_06950"
     CDS             complement(342..620)
                     /locus_tag="PLL28_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNF69098.1"
                     /translation="MSAVFTQEDVLTEQLIDDLIGARLIVHNDDVNTFEWVIQSLVEI
                     CRHSQEQAEQCAYIIHFKGKYAVQQGAKRTLSPMREQLVDRGINATIE"
     gene            704..1201
                     /locus_tag="PLL28_06955"
     CDS             704..1201
                     /locus_tag="PLL28_06955"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HNF69099.1"
                     /translation="MDPRESARSRFKRQVTGLWTFRLFLLSKLPIALITGIRIRALDD
                     NSCTVSVPFKWLSQNPFRSVYFASQSMAAEMSTGVLSMMAIQGCEPPVSMLVTKVHAV
                     FTKKAAARVFFTCNDGEAIRKAVDQTMASGEGVTIVCSSIGKLKDGTIVSSFEVEWSF
                     KARSK"
     gene            1260..1709
                     /locus_tag="PLL28_06960"
     CDS             1260..1709
                     /locus_tag="PLL28_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69100.1"
                     /translation="MHQPKTLLILVPFLFASVFVSCNRTTGKEENNTDEATESTSTSR
                     TKDPNIITVDDPFKNQPNVFCTVIRNLDISYYAPEKTVDDVAAYPKKCCLLDVCLDQP
                     GSSGMVVNLGDDTEIVTADFELIKVFDSADEARAYAEKHQIQDAIFE"
     gene            complement(1790..3076)
                     /locus_tag="PLL28_06965"
     CDS             complement(1790..3076)
                     /locus_tag="PLL28_06965"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNF69101.1"
                     /translation="MSETAHIKLGEQEIDLPVFTGSEGEQSIDINKLRDLTGYVTLDS
                     GFKNTGATKSSITFLDGELGILRYRGYPIEQLAEKSNFMEVTYLLLYGELPTNEQYIE
                     FTDRITRHTLVHEDIKQFFDGFPSNAHPMGQLCSLMCTLSSFYPDALNPHMSANEVDM
                     TITRILAKMPTLVSWIHKKSVGHPIMYPQNKFDYVTNFLYMVFGMRTEEYMPDPVVVD
                     ALDKLLILHADHEQNCSTSTVRMVGSSQANAYASVAAGVAALWGPLHGGANQQVIEML
                     EVIKRDGGNVAKYVEKAKDKNDNFRLMGFGHRVYKNFDPRAKIIKEACDNVLNKLGIH
                     DPVLDIAKQLEEVALRDNYFVERKLYPNVDFYSGIIYRAIGFPTEMFTVLFALGRLPG
                     WLSQWKEMRESGEPIGRPRQIYTGPTQRDYVNIAIR"
     gene            complement(3226..3549)
                     /locus_tag="PLL28_06970"
     CDS             complement(3226..3549)
                     /locus_tag="PLL28_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69102.1"
                     /translation="MEPLSNQQDQQQQHNPNLFWEGFFYLSFFVFFDIVVIPYAPKSI
                     TFDQYYPLAMVIVGGAGLFVGMLAFRIMKNWPNFIKAGLFVVMYGIILWYLIENINKF
                     TVPWM"
     gene            3604..4224
                     /locus_tag="PLL28_06975"
     CDS             3604..4224
                     /locus_tag="PLL28_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNF69103.1"
                     /translation="MKTCPHCHNENPVDFSYCRYCGNALNGVPENKPKSIWKRLPAWV
                     WIILIVGGIAALILTLIGSFIAIATMEGVASIVILICGIVGFGVLPLRRPDRAGSFAR
                     AIGLSFFALMGATVDQTGNFLYNKPIEMCFCHDGTSLTRDIDVTNPIPGQTNITQDYT
                     CYDSTGKAAKTINIFAVLGIRFVEYVILGYLLIALRQVRWRIKNNR"
     gene            4246..4842
                     /locus_tag="PLL28_06980"
     CDS             4246..4842
                     /locus_tag="PLL28_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69104.1"
                     /translation="MKIRNLLLLCSIVVTFDCSAQFISLRATNASQFISDGLYDPDIS
                     KAIGAELIYSKPVNFIKWPINYNIGIDYRNVDGAHAAYVATGLTWVTRTPSGFMFAPN
                     AESVTYVTSNWLHYADVNMYNGVLIGDSTYTYSMGWSLGYNIGYVFGKQFFVHYGFAG
                     RYNTTPQYHNDAIEGASSWDFMLKAGILWKFKRKFYKA"
     gene            complement(4839..5858)
                     /locus_tag="PLL28_06985"
     CDS             complement(4839..5858)
                     /locus_tag="PLL28_06985"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNF69105.1"
                     /translation="MSFAQTFAVDLENPISTSFSFLSSTPNAGVNAMGEAGTAVINNG
                     ALYTNAAAISALPDSFGLALDYKRGAGNLLCLSVYNQYTSSALAFGLRYNSLGGSTLI
                     TPTGNINQGASNYELAFDVAGSHSFSPHFMAAITGKVFYSTISKDMIINGVVIKPSSG
                     IAADISCLYTTTVNGTDKINIGATISNIGSPQEYGYPGYDYFIPTTLSIGFAYDVLFD
                     DNNQLVFALDVNKLIVPTIYNRSESAAYGMLHSFNDAPGGVQEEMHEITLGAGIDYSY
                     QEKVHLRAGYFYEHPSKGGRTYFSLGGGIAIGNGNLDVAYRFYDNTQAIVVANALSLT
                     LSVNL"
     gene            complement(6034..7866)
                     /locus_tag="PLL28_06990"
     CDS             complement(6034..7866)
                     /locus_tag="PLL28_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69106.1"
                     /translation="MHTKLSGFFTWIVFTIVPFIGFAETEPGPNNNAVTYNLPVIDKS
                     HIESDLNHATETLQRISLLGELAAYYVTWEADAHKADSLLSVGIDMAELSYNNEFLLE
                     AYSNYLIYIDDYTFTDKVSRIVPLLESMSGQLTNKKEVWKCKYALAYGCKLIFQTDRA
                     KDYAHQALTEAIRLKDNNLTAESHLLLGSLQHDMNNNVEAIRNYLDALTIAENGSDLE
                     LRLKCYDELSTFYNLIKAYDKSINYKLKELNIAEHAKKPDSMRIMTIKFDMEVIAFNN
                     RTLNENQLYKIIDYSNRNGVSRLKRFALVVFRNHLIKQSNFAELYHLYNEEYPEELEY
                     IRTNDTTTYFRLEAMFNEYEGDVVTAKEYFDKAAQGINRSKDKLRQSSFYLRYGDFLQ
                     RNNFLEEARDCFRRSYTIAASINYYEFMVEATGKLEKIYSLQNNFEEAYAYSLLNKST
                     SESLDSLMQKEQLQILELENEEVLRDQQLQQERDETRRRNNIQYTAITILIATFFLML
                     FILGGIRVSPAIIRFLGFVSFIFFFEFLILLFDTWIHHLTHGEPWKILAIKILLMCGL
                     VPLHHMIEKRVISYIISHKMNLIPKRSPKPVLVEADDVREEEKA"
     gene            7963..8418
                     /locus_tag="PLL28_06995"
     CDS             7963..8418
                     /locus_tag="PLL28_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69107.1"
                     /translation="MQTNQQLSWYKKATIILAITTIIPTTIVLTSQAAEPLPAQQVVT
                     SQPISTEDAARFVANYADQAEKVKAVVKGVVIEAGQLESLNTVAAAHPNANKFRLYFG
                     KDDNGGDVTIAVAMDGQGNDQTDTMYSSARTGTNLCPPVCDANGTIGGN"
     gene            8611..9249
                     /locus_tag="PLL28_07000"
     CDS             8611..9249
                     /locus_tag="PLL28_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF69108.1"
                     /translation="MDIRVVLFDDNKHIRESLQMLISATPGITCIGTYPDCRDMLIYI
                     ESDVPDVVLMDIEMPGMNGIEAVKLIKQHFPGVQILMQTMFDDDDYIFEAICSGASGY
                     ILKTTTPLGYTEAIKNVHAGGSPMSPSIARKVFELFKTNLASQVDVNYNLTDKEKEIL
                     QNLVKGKSYKMIAEEMSISTETVKTHMKNIYTKLHVNSNTEAVVKAIKDKII"
     gene            9369..11174
                     /locus_tag="PLL28_07005"
     CDS             9369..11174
                     /locus_tag="PLL28_07005"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF69109.1"
                     /translation="MIKNSKDEETLADTYNDLAYQFAYSKPDSAIFYGNMALDLSEKH
                     NLQEQIAPAYNAIGLAYQSKGMYQISIDHLTKQLPYLKSDSSALSRTYHNIGLSYKFL
                     GNLNTALEYEIKSAKAIEKSSDPVALGAIYLSIGNIYRDLQDYDLAYSYLQKSLKTYE
                     SGTNFGISSKNSLIAMTYSGIGSLFLVKYEIDSAIYYHSKAIDLLEKEPVSYNLAILI
                     ENLGDDYLVIDEREKAIKQFQRAKQIMEALQSGADVGYEYYKIANVYRIAKQYNKALS
                     YNDSALVLFTKYDAVNFKLASYDLNFRIYDSMGRPAEALENYKLYIALKDSLNSESQQ
                     SEVLRLKEEFETTQKEQRIELLETSNALQLRENERQVMFRNIAIGLIIMLLGFGLLIW
                     NRYRIKQQVKQLQMRNDIAIDLHDEVGSSLSSIKMLSEIAALQKDDKHQLDDLLGRIQ
                     VNASETAEKMHDIIWMINPKHDQLSGILSRMENFMFDMCSPGEISFNLQKNIDPNIKL
                     NMLQRKDLMLIFKEAVNNAVKYAAATNIQAHISYVDHHVQLIVTDNGKGFDLQTIRRG
                     NGLDSMQMRAQELAGTLEINSTPNTGTTIRVRFPI"
     gene            11261..13324
                     /locus_tag="PLL28_07010"
     CDS             11261..13324
                     /locus_tag="PLL28_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69110.1"
                     /translation="MKKQITLFAVCFIAGLLTVNAQTNTFPATGSVGIGTITPNASAL
                     LEMSSTSKGLLAPRMTKAQRDAIASPATGLLIFQTNSSPGFYYYTGASWTALASKGAN
                     TTLSNLTTTSISQPLLPNADNTVDLGSSTLRWNELYVNNIKFLDGTTQTTAGGGGATY
                     TAGTGISIAGSVISNTGDVNAADDLTILTNHGGDVTGVYNNLQISTGAVGSAEIADGS
                     VTSTDILNGTIAAADLSSMGATSGQVMQWNGIAWIATTPAAGAETDPQVGTISTNAIP
                     RWDGTALTTGGIVDDGDNLSFGWNNGISSNTTASFYNNESVITDEDIAVKGVDQTVAV
                     GGTATVHSTGYLGYNTVGVVFGDTPVSHVGVWGNASTTTNSAAVYANNSSTGSDNYGV
                     VAKSVGAGTTNIGLYARSAGATNNYGVIVPTDGGKSGFNWSSPSALVGIKGNDTDNAL
                     RIIGTDFNTDLIVDEVGRVGVHGDPDAFSANFNVYGSGYVQDRLNIGVSGAFLYSMLA
                     VNGTDETVTIMGTNPYIQMKNAGSNVGYLRANGSDLMLATNSGVTGKVILRANGINSM
                     YVDQNGNIVMGSTTVAPKSGYKLSVDGKVVCEELLVQLSPWPDYVFADDYKLSSLSEV
                     ENFINENNRLPGIPSAAEVESEGLNVGQMQKLMMEKIEELTLYMIELQKQNADLQAKV
                     DALSN"
     gene            13350..15041
                     /locus_tag="PLL28_07015"
     CDS             13350..15041
                     /locus_tag="PLL28_07015"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNF69111.1"
                     /translation="MKNQITKSIIAICLMLCTQWLPAQTIEISRPEKIIGCEEVPASL
                     LAPNGKLAHANNPMYYNSTTIGNKMDLTPKLDWPLKMSDEYKDLSGVYSYFYISNYLD
                     LDPDTTVKEDWYCFTNDYARNYEGHNGADICPYPYGWQMMDDESVDVIAAADGVIGAI
                     FDGNTFDRNCSKPHTFIINDTLDNTGYLGNYVRIDHAGGLTTLYGHLKHGTLTDLNVG
                     DSVKSGDYLGKIGSSGNSTGPHLHFQVMWYGSLVEPWKGNEDVGEPNCNFTYYQSLWN
                     NQIPYYNTQLIRVATHFGAPTETSCTNYEAGSNETETLVNHFSSFQIAKIGVSIRNIF
                     AGMPINVDIFTSTGVLKNSMSYTPLVFDEIATYYFDQSLLSYTTGTYRVRVTFDGKTY
                     DHYFTVNCSGDLIYSAAQSGHKGFISGGSITSTSTISGTSANEILYEAETFVKLNVGF
                     QAAMNCEFKARLDDCTLGGVKEEPEPIKSSDSRLTVYPNPTFGEFSLLYEGTSENDAL
                     VNIFDLTGRLITQYTFSSNQGNVNHTFDLSEQAKGLYFIEVRQGDNVSTSQLIIQ"
     gene            complement(15098..15838)
                     /locus_tag="PLL28_07020"
     CDS             complement(15098..15838)
                     /locus_tag="PLL28_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69112.1"
                     /translation="MQTTTHTTSRSNQGLVILGLVMSVSLFFSCAAKSKEEPVLDGRV
                     FTVLTIPDGNSAEAAMERISFTNGKFDNDNCHLWGFGDGAYAAKEAGDSIAFTATTTS
                     AKEGTMTWTGVIKGTTLMGTMVWSKEGQSDLNYHFTSANIEEVDLNGISFAVQFNDGD
                     TAQAETITFNNGMFESPGCYTYGFSASPYQAYMLDGKQHFQCLYTSAKEGKMLFYGVI
                     DGTSISGTQFWTKEGQDDVYYKMSGQKL"
BASE COUNT         5077 a         3258 c         3344 g         4839 t
ORIGIN      
        1 gatccaagta gactcatcat ttttccgttc gatctgcgcg caggacttaa taaccgattc
       61 aaaattttta tctaaagtga catcaaacgc tttctttttt aaaagttgtt tcatgcttcc
      121 ggagatatgc agattttgga aatccaatat gcatctcgga tccggcgcaa accaatacgg
      181 ttcatgtccc aaggtgaacc atggaaaaat tccggaccgg tatgccaaca acaatctatc
      241 agaagataaa tcgccgccga tagcgagcag accatccggg tcggcatcaa acgggtcggg
      301 aaaaatattg cgatgatcga gttgccagat tggcatttcg cttattcgat ggtggcgtta
      361 attccgcgat caacgagttg ttcgcgcata ggtgacaacg ttcttttagc gccttgttgc
      421 acggcatatt tccctttgaa atggatgata tatgcgcatt gttcggcttg ttcctgggag
      481 tggcggcaga tttcaaccag gctttgaata acccattcga aggtattcac atcgtcgtta
      541 tgtacaataa gacgtgcgcc gatcaggtcg tcgatcaact gttcggttaa tacatcttct
      601 tgcgtaaata ctgcgctcat gccttgaact ttaggataca aaattaacaa acgaacagtt
      661 attcatggct cctggttcaa aaagttggct aatttcgagc cttatggacc cacgtgaatc
      721 cgccagatcc cgcttcaaac ggcaggttac aggcttatgg acctttcggc tttttctgtt
      781 gtcgaaactg cctatagccc tgatcaccgg catcagaatc cgcgccctgg atgacaatag
      841 ctgcactgtt tcggtgccgt tcaaatggct gtcgcagaac cctttcagat cggtatactt
      901 cgcttcgcag tcaatggcag cagagatgtc gaccggcgtg ttaagtatga tggccattca
      961 gggatgtgag cctcctgttt cgatgttggt gacgaaagtg catgctgtct tcacgaaaaa
     1021 ggctgctgca cgggtatttt ttacgtgtaa tgatggggaa gccattcgta aggctgtaga
     1081 tcaaacaatg gcgagcggag agggcgtaac tattgtttgc agtagtattg gaaaactgaa
     1141 agacgggact attgtcagca gttttgaagt tgaatggtcg tttaaagcaa gaagcaaata
     1201 atatttatgg ttactattta cgtttattaa taattacact tcaagaaaat catcgcaata
     1261 tgcatcaacc caagactctt ttaatccttg ttccatttct gtttgcctcg gttttcgtat
     1321 cctgtaatcg cacaacaggc aaagaagaaa acaacactga tgaggctaca gaaagcacat
     1381 caacatcacg caccaaggat ccaaatatta taacagttga tgatccattt aaaaatcagc
     1441 caaatgtgtt ttgcacagtg atcaggaatc tcgacatcag ttattatgca cctgaaaaaa
     1501 cggttgatga tgttgctgcc tatcctaaaa aatgttgcct gctggatgtt tgtctcgatc
     1561 agccgggctc ttcaggaatg gttgtaaatt tgggagacga cactgaaatt gtaacagctg
     1621 attttgagct tataaaagtt ttcgattctg ctgatgaagc gcgtgcatac gccgaaaagc
     1681 atcaaattca ggatgcgatt tttgagtagt taaaattgta tttcagcata taaaaaaagg
     1741 agacttgatg aagcaagtct cctttattat ttggttaagg ctgtttatct caacgaatag
     1801 cgatattcac ataatcgcgt tgtgttggtc ctgtatagat ttgacgcggg cgaccgatag
     1861 gctcaccgga ttcacgcatt tctttccatt gtgacaacca tcccggtaag cgaccgagtg
     1921 caaacaacac ggtgaacatt tcagttggga atcctattgc acgatagatg ataccggaat
     1981 agaaatcgac attcggatat aattttcttt ccacaaaata gttatcgcgc aaagctactt
     2041 cttcaagttg tttagcgata tcgagaacag gatcatggat accaagttta ttcaacacgt
     2101 tatcgcatgc ttctttgatg attttagcac gtgggtcgaa atttttatat acacggtgac
     2161 caaatcccat caaacggaaa ttatcatttt tgtcttttgc tttttctaca tatttcgcaa
     2221 cgtttccgcc atcgcgtttg atcacttcca acatttcaat tacttgttgg ttggcaccac
     2281 cgtgaagtgg cccccaaagt gcggcaacac ctgctgctac gctggcataa gcattagcct
     2341 ggcttgaacc aaccatgcgc acagttgatg tagagcagtt ttgttcgtga tcggcgtgga
     2401 ggataagcaa tttatcaagt gcatctacta ctacaggatc cggcatatat tcttcggtac
     2461 gcataccaaa caccatatac aggaagttgg taacatagtc gaatttattt tgcggataca
     2521 taattggatg accaacagat tttttatgaa tccagctaac gagtgttggc atttttgcga
     2581 ggatacgggt aatagtcata tccacctcat ttgcactcat atgaggattc agggcatccg
     2641 gataaaaaga gctgagcgta cacatcaacg aacacaactg acccattgga tgggcattag
     2701 acgggaagcc atcgaaaaat tgtttgatgt cttcatgtac aagtgtatgg cgcgtgatac
     2761 gatcagtgaa ttcgatatat tgctcattgg taggcaattc gccataaagc aacagatatg
     2821 ttacttccat gaagttcgac ttctcggcga gttgttcgat cggataacca cgataacgca
     2881 gaatacccaa ttcaccatct aaaaaagtga tgctgctttt agtagcacct gtgtttttga
     2941 atccgctatc gagcgtaacg taaccggtaa ggtcgcgcaa tttgttgata tcgatcgatt
     3001 gttctccttc ggaaccggta aacaccggca agtctatctc ttgttctcca agtttgatat
     3061 gcgctgtctc agacataatc ttcgtgcacg tttggtttgg tagtgtaaaa atttgccata
     3121 caaaggtata aaatgtatgc acagatatgc ctgttgggct acactttttt taaagtgcaa
     3181 ggatgataag ggtcgaaatt tatctaaaat gtaaagaaag tggcattaca tccacggaac
     3241 cgtgaatttg ttgatgtttt cgataagata ccacaggatg ataccataca tcaccacaaa
     3301 gaggccagct ttaataaaat tgggccagtt tttcatgatt ctaaacgcca gcattccaac
     3361 aaaaagccct gcgccgccaa caattaccat agcaaggggg taatattgat cgaaggttat
     3421 agatttaggg gcatatggga ttacaacaat atcgaaaaac acgaagaagc tcaagtaaaa
     3481 aaagccttcc caaaagaggt tcgggttgtg ttgttgttgc tgatcttgtt gatttgacag
     3541 gggttccatc gcctgcaaag gtaaggaaga atgcattaat ggagatatcc tatttttgct
     3601 tgcatgaaaa cctgcccaca ttgtcataac gagaacccgg tggacttcag ctattgcaga
     3661 tactgtggta acgctctgaa cggagttccg gaaaacaagc ctaaaagcat atggaaacgg
     3721 ctacctgcct gggtttggat cattctgata gttgggggta tagcagcctt gattttaacg
     3781 ctcatcggtt ctttcatagc aattgcaaca atggagggag ttgccagtat tgtgattttg
     3841 atttgtggaa tcgttggatt tggcgtttta ccccttcggc gacctgacag agccggaagt
     3901 tttgccagag ctatcggact gagctttttt gcactcatgg gagccacagt agaccaaaca
     3961 ggcaatttct tatacaataa accgatagaa atgtgttttt gtcatgatgg aaccagcctt
     4021 acaagagata tagatgttac caatccaatc ccgggacaaa cgaatattac acaggactat
     4081 acttgttatg atagcaccgg gaaagcagcc aagacgatca atatttttgc cgtattggga
     4141 ataagatttg tggaatatgt gatactgggt tatttgctca ttgcattacg acaggtcagg
     4201 tggcgaatta aaaataaccg gtaatctttt aacgttttgt attgcatgaa gattagaaac
     4261 ctccttctat tgtgcagcat tgtagtaaca tttgactgca gcgcccaatt catttcactt
     4321 cgcgctacaa atgcatcaca atttattagc gatggattat atgatccgga tatttcaaag
     4381 gccattggcg cagaattaat ttatagcaag ccggtgaatt ttattaaatg gccaataaat
     4441 tataatatcg gaattgatta tcgcaatgtt gatggtgcac atgctgcata tgtggcaacc
     4501 ggattgacat gggttaccag aacaccttca gggtttatgt ttgcaccaaa tgcggagagt
     4561 gtaacatatg taacaagtaa ttggctgcat tatgctgatg taaacatgta taatggtgta
     4621 ttgataggtg attctacata cacctattct atgggctggt cacttggata taatatcggc
     4681 tatgtattcg ggaagcaatt ttttgtgcac tacggatttg caggtagata taatacgact
     4741 ccgcaatatc ataatgatgc cattgaggga gcatcttcct gggatttcat gctgaaggcg
     4801 ggaatccttt ggaaattcaa acgcaaattt tataaagctt agaggtttac cgacagtgtg
     4861 agcgaaagtg catttgctac aacaattgcc tgtgtattgt cataaaaacg ataagctaca
     4921 tccagatttc cgttgcctat tgctatgcca ccgcccaggg aaaaataggt gcgtccgcct
     4981 ttagagggat gttcataaaa gtatcctgcc cgaaggtgta ctttttcctg ataactataa
     5041 tctattcccg ctcccaaagt aatttcgtgc atttcctctt gcactccacc cggggcatca
     5101 ttaaaggagt gtaacatgcc atatgcggca gattctgatc tattgtatat tgtaggaaca
     5161 atcagtttat ttacatctaa tgcaaataca agttgattat tgtcatcaaa aagtacatca
     5221 tatgcaaagc caatactaag tgtagtcggg ataaaataat catagcccgg atatccatat
     5281 tcttgtgggc taccgatatt actaatggtg gcaccaatat tgattttatc agttccgtta
     5341 actgttgttg tatataagca ggaaatatct gcagctattc ctgatgaagg tttgatcaca
     5401 acaccattaa taatcatgtc tttagaaatg gttgaataaa atactttccc tgtaatagca
     5461 gccataaaat gtggcgaaaa tgaatgcgat ccggcgacat caaatgccaa ttcataattc
     5521 gatgcaccct ggttgatatt tcctgttggt gttatcaatg ttgaacctcc cagggaatta
     5581 tatcgcaatc caaatgcaag agcgcttgaa gtgtattgat tgtataccga caaacacaac
     5641 aagttaccgg cgccgcgttt ataatcgaga gcgagaccaa aggaatcggg caatgcactg
     5701 attgctgcag catttgtgta caaagctccg ttatttatca ctgcagtgcc ggcttcaccc
     5761 attgcattaa ctcctgcatt tggtgtactt gataaaaaag agaatgaagt agagatcgga
     5821 ttttcaagat caacagcaaa agtttgtgca aatgacattt caatactaca acacatgagt
     5881 accaccgaaa aaataccaca agaaattagt ttagaaatgc ttatcatacc ttgttaaaat
     5941 aaatgatatc aaaattacaa gatatttcca ttgtgaaaaa taatttgaac acctcacaag
     6001 ccaaattcta tgtatttaat aagtgcgtcg gcctcaagct ttttcctctt ccctcacatc
     6061 atcggcttca accaatacag gttttggcga gcgttttggt atgagattca ttttatggct
     6121 gataatatag gaaatcaccc ttttttcaat catatggtgc aggggaacca gtccgcacat
     6181 cagtaaaatt tttatcgcca aaattttcca tggctcaccg tgcgttaaat gatgtatcca
     6241 tgtgtcgaac agcaagatta aaaattcaaa gaagaagata aaggaaacaa atccgagaaa
     6301 cctgatgatt gcaggagaaa ccctgatccc tccaagtatg aaaagcatca gaaaaaaggt
     6361 agctataagg atagtaatag cggtgtattg aatattgtta cgtcttctgg tctcatcgcg
     6421 ttcttgttgc agttgttgat cccgcagcac ttcttcattc tccagttcca gaatttgcaa
     6481 ctgttctttt tgcattaagc tatcaaggct ttctgaggtg cttttattca gcaaactata
     6541 tgcgtaagcc tcctcgaaat tattttgaag tgaataaatt ttctcgagct tacctgttgc
     6601 ttcaaccata aattcataat aatttataga tgctgcgata gtatatgacc ttcggaaaca
     6661 atctctggct tcttccagaa aattattgcg ttggagaaaa tcgccatatc tcaaataaaa
     6721 gcttgactga cgcaatttat cctttgacct gttaatccct tgtgctgctt tatcaaaata
     6781 ttcctttgca gttacaacat ccccttcata ttcattgaac attgcctcga gcctgaaata
     6841 agttgttgtg tcgttagtcc tgatatattc cagttcctcc ggatattcct cattgtacaa
     6901 atgatataat tccgcaaaat tactttgttt aataagatgg tttcggaaca caaccagggc
     6961 aaatcgtttc aggcgactga cgccatttcg attgctgtaa tcaatgattt tatataactg
     7021 attttcgttt aaggttctat tattaaatgc aataacttcc atatcaaatt taatggtcat
     7081 tatacgcatt gaatccggct tttttgcatg ttcagcaata tttaattctt tgagtttata
     7141 gttaatcgac ttgtcataag cttttatcaa attataaaat gtagatagtt catcatagca
     7201 tttcagacgc aactccagat cgctaccatt ttccgcaatg gtcaaggcat cgagataatt
     7261 acgaattgcc tcaacgttgt tattcatatc atgttgcaat gagcccagca aaagatggct
     7321 ttcggcggta aggttgttat cttttaagcg tattgcttct gtcaatgcct ggtgtgcata
     7381 atcctttgca cgatctgttt gaaaaattag tttacaccca taggcaagag catacttaca
     7441 tttccatacc tcttttttat tggtaagctg acctgacatg ctttccaata aagggactat
     7501 gcgactcact ttatctgtga aggtataatc atcaatataa atcagataat tcgaatacgc
     7561 ttcaagtaag aattcattgt tatacgacag ttcagccatg tcaattccca cactgagtag
     7621 gctatcggcc ttatgtgcat cagcttccca tgtaacataa taggccgcaa gttcaccaag
     7681 cagcgatata cgttgcaacg tttctgttgc gtgatttagg tcgctttcta tgtgagattt
     7741 atcaataaca ggcagattgt aggttacggc attgttatta ggcccgggct ctgtttcggc
     7801 gaatccaata aagggtacaa tagtgaaaac gatccaggtg aaaaacccgg acagcttggt
     7861 gtgcatggtc atgtttgaag taaggagtct tgtgattagg tcaaatgtgt atgccggtat
     7921 agtttgtatc tttaaccaaa catacaacca aaaactcaca ttatgcaaac taaccaacaa
     7981 ctctcatggt ataagaaagc taccattatc ctcgcaatta caaccatcat ccctacaact
     8041 atcgtgctta cttctcaagc tgctgaacca ctaccggcac aacaggtagt taccagtcaa
     8101 ccgatctcaa ctgaggatgc tgcaagattt gtagctaatt atgcagatca ggctgaaaaa
     8161 gtaaaagctg ttgtcaaggg agtcgttatt gaggctggcc aattggaatc cttaaataca
     8221 gttgcggcag cccaccccaa tgcaaataaa ttccgcttgt attttggaaa agacgataac
     8281 ggaggcgacg tgacgatagc agtggcaatg gatggtcagg gtaatgacca aacagatact
     8341 atgtattctt ctgcaagaac aggcaccaat ttatgtccac ctgtttgcga tgccaatggt
     8401 actattggtg gcaattaatc acatttcttc tcataaacgg cgatcattgt ttaatacttt
     8461 ggtcgccgtt ttgtttttgg gaaccggctt tgcgatagtg taaatcggct aattccggat
     8521 aaaatacaac tgttcaaagg aaacgaatat ccttatatca cccaaattcg gtatgtttag
     8581 gtcattaatc agactatact tttgcagccg atggacatcc gtgtagtttt atttgacgac
     8641 aacaagcata tcagggagag cttacaaatg cttatttccg caactccggg tattacctgt
     8701 ataggaacct atcctgattg cagagacatg cttatttata tagaatcaga tgttccggat
     8761 gtggttttga tggatattga aatgcccggc atgaatggca tagaggccgt aaaacttatc
     8821 aaacagcact ttcccggtgt tcagatactg atgcaaacca tgttcgatga cgatgactat
     8881 atctttgaag cgatatgcag cggtgcatcg gggtatatat taaaaaccac cacgccatta
     8941 ggttacactg aagccatcaa aaatgtacat gccggaggtt cacctatgag tccatcgatt
     9001 gctcgaaagg tgtttgaatt attcaagacc aatctagctt cacaggtgga tgtgaactac
     9061 aatctgacag acaaagaaaa agagatactt cagaacttgg taaaaggcaa gagctacaaa
     9121 atgattgctg aagaaatgtc aatctctact gaaactgtta aaacccatat gaagaatatt
     9181 tacaccaaac tgcatgtaaa ttcaaataca gaagcggtgg tgaaggcgat aaaggataag
     9241 atcatctaac aggcttgaat caaaagtatc gtatgacaag atggacagcg cacatttaca
     9301 tttttttgtt atcgatttgt tggataaata atatatctgc acagcaggat attgattcac
     9361 tacggaaggt gattaaaaac tccaaggatg aagaaaccct tgcagacacc tataatgatc
     9421 tggcatatca gtttgcatat tcaaaaccgg atagtgccat attttacgga aacatggcac
     9481 tggacttatc tgaaaagcat aaccttcaag aacagatagc tccggcttat aacgccattg
     9541 ggttggctta tcagagcaaa gggatgtatc agatttctat tgatcatctt acaaaacaat
     9601 taccatattt aaaaagcgat agttcagcat tatcaagaac atatcataat atcggattgt
     9661 cttataagtt tttaggcaat ctaaatactg ctctggaata tgaaattaaa tcagcaaaag
     9721 caatagaaaa aagttcagac cctgtagcat tgggagctat ttatttatcg attggaaata
     9781 tttatcgcga ccttcaagac tatgatcttg cctattctta tcttcaaaaa tctcttaaaa
     9841 catatgaatc agggacaaac tttggcatta gcagcaaaaa ttcgctgatt gcaatgacct
     9901 attcaggaat tggtagttta tttctggtaa aatatgaaat agacagcgca atttactatc
     9961 actcaaaggc tatcgattta ttggaaaagg aacctgtttc ttacaattta gcaatactca
    10021 tcgagaatct gggtgatgat tatttagtta ttgatgaaag ggaaaaggca atcaaacaat
    10081 ttcagcgtgc aaagcagatc atggaagcat tgcaatcagg tgccgatgta ggttacgaat
    10141 actataaaat tgccaacgta tatcgaattg ccaagcaata taataaggca ttgagctaca
    10201 acgattcggc acttgtatta tttaccaagt atgatgccgt aaatttcaaa ttggcgtctt
    10261 atgatttaaa ttttcgtatt tacgattcca tggggcggcc ggcggaagct ttagaaaatt
    10321 ataagcttta catagctttg aaagacagct tgaattcaga atcacaacaa tcagaagtac
    10381 tgagattaaa agaagaattt gaaacaaccc aaaaagaaca gcgcattgag cttttagaaa
    10441 ccagcaacgc cttgcaattg cgcgaaaatg agcgtcaggt gatgttccgc aatatagcga
    10501 ttggcctgat tatcatgttg ctcggttttg gtttattaat atggaacaga taccggatca
    10561 agcagcaggt aaagcaacta cagatgcgca atgatatagc gatagacctg cacgacgaag
    10621 ttggaagttc gttgagttcc ataaaaatgc tcagtgagat tgcggcatta caaaaagatg
    10681 ataaacatca acttgatgat cttcttggcc gcatccaggt caatgcatca gaaaccgctg
    10741 aaaaaatgca tgatatcata tggatgatca atcctaagca tgaccagtta tccggcattt
    10801 tatcgcggat ggaaaatttc atgtttgaca tgtgttctcc tggcgaaatt tcattcaacc
    10861 tgcaaaagaa catagatccg aatatcaagc tcaatatgct tcagcgcaaa gacctgatgc
    10921 tgatatttaa ggaagccgta aataatgcgg tgaaatatgc tgctgcaaca aatatccaag
    10981 cacatatctc atatgtcgat catcatgtgc aattaatcgt taccgataat ggtaaaggat
    11041 tcgatttgca aacgatcaga cgcggtaatg ggcttgatag catgcaaatg agagcacagg
    11101 aattagctgg cactttggag attaactcca cgccaaatac aggcactact attcgtgtgc
    11161 gttttcctat ttgacccact cccccattct cggtatttta caggtgcatt catggaaata
    11221 gctttgctga tacaatttaa cctatcacaa aaactattct atgaaaaagc aaattacgct
    11281 attcgccgtg tgttttatag caggactact cactgtaaac gcacaaacca atacatttcc
    11341 cgcgacagga agtgttggca ttggcaccat aaccccaaat gcatcagcat tgctggaaat
    11401 gtcttcaacc agcaaagggt tattggcgcc acgtatgaca aaagcacaaa gagatgctat
    11461 tgcatcacct gcaacaggac tgctcatttt tcaaactaac tccagtcctg gattttacta
    11521 ttatacggga gcatcatgga ctgcccttgc ttcaaaaggt gcgaacacaa cgttgagcaa
    11581 tctcaccact acatctattt cgcaaccatt attgcctaat gcagacaata cagtagattt
    11641 aggaagtagc actttgcgct ggaatgaatt atatgtaaat aatattaagt ttcttgatgg
    11701 cactacacaa actactgccg gtggtggtgg tgctacctat acagcaggaa caggcatttc
    11761 aatcgcaggc tctgtaatct caaatacagg cgatgtaaat gctgctgatg atttaacaat
    11821 tctgactaat catggaggag atgtaaccgg tgtttataac aaccttcaaa tttcaacagg
    11881 agcagttgga agtgccgaaa ttgctgatgg atcggttact tctacagata tcttaaatgg
    11941 tacaattgct gcggctgact taagttctat gggtgcaaca agtggccagg tgatgcaatg
    12001 gaatggaata gcatggattg ccactacccc tgcagcaggc gcagaaactg atccacaggt
    12061 aggaacaatt tcaacaaatg ccattcctcg ttgggacgga accgcgttga caaccggcgg
    12121 aattgtagat gatggcgaca atctttcttt tggctggaac aatggtatat cctcaaacac
    12181 aacagcatca ttttataata atgaaagtgt tataaccgac gaggatatcg ccgttaaagg
    12241 cgttgatcaa actgtggccg ttggtggcac agcaacggta cattctaccg gttatttggg
    12301 atataacact gttggtgtag tatttggcga tacacctgtt tcacatgtcg gtgtatgggg
    12361 caatgcttct acaacaacaa attcagctgc cgtatatgcg aataacagtt caacaggatc
    12421 agacaattat ggtgttgttg caaaaagtgt tggcgccgga acaacaaaca tcggtttata
    12481 tgcaagaagt gctggtgcaa caaataacta tggtgtgata gttcctaccg atggcggtaa
    12541 atcaggtttt aactggtcat caccttcagc gctcgtgggc attaaaggca atgatactga
    12601 taatgcatta cgtatcatcg gaacagattt taatactgac ttaattgttg atgaagttgg
    12661 ccgtgttggc gtccatggcg atccggatgc atttagcgct aatttcaatg tttatgggtc
    12721 aggatatgtg caagatcgtc taaatattgg tgtcagcggg gctttccttt attctatgct
    12781 cgcagtaaat ggcacagatg aaactgtgac aattatggga acgaatccat atattcaaat
    12841 gaaaaatgcc gggtcgaatg taggatacct tcgtgcaaat ggttctgatt taatgctggc
    12901 aactaattca ggcgtaacag ggaaagttat cctacgtgca aatggtataa acagtatgta
    12961 tgttgatcaa aatggtaaca tcgtgatggg cagtacaaca gttgctccta aatccggata
    13021 taaattaagt gtagacggga aagtagtatg cgaagaatta ctggttcaac tttcaccatg
    13081 gcctgattac gttttcgcag atgattataa attatcttca ttgagcgaag ttgaaaattt
    13141 cataaatgaa aacaatcgcc tccccggcat tccatcagct gctgaagtag aatccgaagg
    13201 attaaatgtt ggtcaaatgc agaaactaat gatggaaaag attgaagagt tgacattgta
    13261 tatgatcgaa cttcagaagc agaatgccga cttgcaagct aaagttgatg ctttgagtaa
    13321 ttaattattt caatcaacaa ctgatcatta tgaaaaatca aatcaccaaa agcattattg
    13381 caatctgcct gatgctttgc acgcaatggt tgcccgcgca gactattgaa ataagcaggc
    13441 cggaaaaaat aataggttgc gaggaagttc cggcttcatt gcttgctcca aatggcaaat
    13501 tagcacatgc caacaatcca atgtattata acagtacaac aatcggaaat aaaatggatt
    13561 taacaccaaa actcgattgg cctttaaaaa tgtcagatga gtataaagac ctgtccggtg
    13621 tatattcata tttctacatt tcaaattatc tcgatcttga tcctgatacc actgttaaag
    13681 aagactggta ttgcttcaca aatgattatg ccagaaatta tgaaggccat aacggtgccg
    13741 atatttgccc ttacccttat ggatggcaaa tgatggatga tgaaagtgtg gatgtcattg
    13801 cagctgctga tggcgttatc ggcgcgatat tcgatggaaa tacctttgat cgaaattgca
    13861 gtaagccaca tacttttatt attaatgata cgcttgacaa taccggatat cttggcaatt
    13921 atgtacgcat tgatcacgca ggagggctta cgacactcta tggtcactta aagcatggta
    13981 cactaacaga tttaaatgta ggggactctg taaaatcagg cgactatctg ggtaaaattg
    14041 gcagctcggg aaattcaaca ggacctcact tacactttca agtaatgtgg tatggctcgc
    14101 ttgttgaacc atggaaagga aatgaagacg ttggagaacc caattgcaac ttcacttact
    14161 atcaatctct ctggaataat caaattcctt actataatac acaactcatt agggtagcta
    14221 cacattttgg agcaccaaca gaaacgagtt gcacaaatta tgaagcggga agtaatgaga
    14281 ccgaaacatt ggtaaatcac ttctcttcat ttcaaattgc taaaattggc gtttcgatac
    14341 gcaatatctt tgccggaatg ccaatcaatg tcgacatttt cacaagcacc ggagtgctta
    14401 agaacagcat gtcttatacc cctttggtat ttgatgaaat tgccacttat tacttcgatc
    14461 aaagcctctt aagctatacc acaggcactt atagagttcg tgttacattt gatggcaaaa
    14521 cctatgatca ttactttact gtaaattgca gcggtgatct catttattcc gcagctcaaa
    14581 gtggtcataa aggatttatc agtggcggaa gcataacaag cacgtctaca atttcaggca
    14641 ccagtgcgaa tgaaatcctg tatgaagcag aaacctttgt aaaattaaac gtaggatttc
    14701 aagccgcaat gaattgtgag ttcaaggccc gattagacga ttgcacactt ggaggcgtta
    14761 aggaagaacc cgaaccaata aaatcaagtg attccaggtt gacggtttat ccaaatccaa
    14821 ctttcggaga attctcttta ttgtatgaag gaacctctga aaatgatgct ttggtaaaca
    14881 ttttcgatct taccggaagg ctcattactc aatatacctt ttcgtcaaat caaggcaatg
    14941 tcaatcacac ctttgatctc agtgaacagg caaagggctt gtattttatc gaagtccgac
    15001 agggtgacaa tgtaagtaca tcacaattga ttattcaata aaaaaatatc aggcaggtat
    15061 taaaccatat tcctgcctga ctttttttct accataatta aagcttctgc cccgacattt
    15121 tgtagtaaac gtcatcctga ccttccttcg tccaaaattg tgttcctgaa atcgaagtgc
    15181 catctattac tccataaaat aacattttcc cttctttcgc actagtatat aaacattgaa
    15241 aatgttgctt gccatccaac atatatgcct ggtagggtga agcactaaaa ccataggtgt
    15301 aacaacccgg actttcaaac atgccattat taaatgtgat tgtctcagct tgagccgtgt
    15361 ctccatcgtt aaattgtaca gcaaaactta ttccattgag atcaacttcc tcaatatttg
    15421 cactcgtgaa atggtagttt aaatcagact gcccttcttt cgaccaaacc atggtgccca
    15481 ttaatgttgt tccttttatg actccggtcc atgtcattgt gccttcttta gcgcttgtgg
    15541 tagtagcagt aaatgcaatg ctgtcgccgg cttcttttgc agcataagcg ccatcgccaa
    15601 agccccataa atggcaatta tcgttgtcaa attttccgtt tgtaaaagaa attcgctcca
    15661 tcgctgcctc tgcgctattg ccatcaggaa tggtcagcac ggtgaaaact ctgccatcga
    15721 gtacgggctc ttccttagat tttgcagcac aggagaagaa aagtgagaca gacattacta
    15781 atccaaggat caccaatcct tgatttgatc tactggttgt gtgtgttgtt gtttgcataa
    15841 taaatgagtt atgaggtaaa ctgattaaaa tatttggaaa ttatactgaa acctaatcag
    15901 gcgaacataa attgatgccc ctcttcgtac attaaattta taaaacagtc catcttcccc
    15961 aaagtcagca attgtaaaca agatactgaa acctcatgag agtttccggc taaagaacac
    16021 atttattaaa gttaaaattg caaaccatcc ataaatacct ttcgcattta attatttcta
    16081 atatatgttt tctattcaat aatgcatata tatttggttc gaccttttaa tttttttcat
    16141 atataaaaaa gctataccct cttaacaaca attagattaa tttttcgggc ttgaatagca
    16201 tcctatcatt atcgaattta gaaataccac ttttcagtga ttttttacaa ttacagtggc
    16261 agaatagccg aaatcagaag attacagggt agcagccagg cgtttcttaa cattttcaac
    16321 gaggggttta aaatccaaat acctatctta taaatcgata gccatgttat tagtttatct
    16381 ttagccttca ttcgagataa agcaattaac tatgaaaaaa ttactcctca ttctttgtct
    16441 gctattattg gcaggagatc ttttcacaca aacaaacact ttccctgcta caggaaatgt
    16501 tggcattggc accacaac
//
LOCUS       DAOQHY010000065        16339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1666_length_16339_cov_5.718865, whole genome shotgun sequence.
ACCESSION   DAOQHY010000065 DAOQHY010000000
VERSION     DAOQHY010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16339
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1666_length_16339_cov_5.718865"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /gene="aat"
                     /locus_tag="PLL28_07025"
     CDS             <1..425
                     /gene="aat"
                     /locus_tag="PLL28_07025"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HNF69113.1"
                     /translation="LDKDFESVIKSCAQIERKNDESTWIDNYFIDAYCKLHHQGHAHS
                     VEVWQNEKLVGGLYGIIQGGVFFGESMFSRVSNASKYGFISLVAHLQKHQFDFIDCQI
                     YNDHLASLGAHNISREEYMLRLHNGLYKNATPVVPETL"
     gene            complement(427..1071)
                     /gene="pdxH"
                     /locus_tag="PLL28_07030"
     CDS             complement(427..1071)
                     /gene="pdxH"
                     /locus_tag="PLL28_07030"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HNF69114.1"
                     /translation="MEQNEGKLHDMRTDYTTGVFDEQNAYPQPMQQFGLWFEEAIKAG
                     ISEANALVLSTVSNEGKPSSRVVLLKEYVSAGFIFFTNYNSRKGKELAANPYACMNFW
                     WGAMERQVRINGRMEKIPATESDAYFYSRPVGSQAGAMISPQSEVIEGREWLEQQFIA
                     QQLKGELKRPEHWGGYILIPEEFEFWQGRSNRLHDRLQYLKHSDGSWKIHRLAP"
     gene            1146..1739
                     /locus_tag="PLL28_07035"
     CDS             1146..1739
                     /locus_tag="PLL28_07035"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNF69115.1"
                     /translation="MRSWLMPFTRISAILLVLVCSLKHGEAQQYAQLSGIITDNSTGY
                     AVPYASVTVPDDIRGVSASEIGFFTIVVKPLDTLQFSALGYKPKLYVVPDTMHDDLVS
                     IAVSLQRDTIELDSFVVYPWPSREDFREAFLAYQETQPYVMQPIPGIRGKELIDTVPK
                     PPSPIMNPISFFYDEVVKPIQWRKPKRHMVDELPEWK"
     gene            1957..3216
                     /locus_tag="PLL28_07040"
     CDS             1957..3216
                     /locus_tag="PLL28_07040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HNF69116.1"
                     /translation="MTSRNIIILGAGSAGTMMANHLRGKLDRNEWNITIVDEVEIHYY
                     QPGFLFLPFGIYDEHEIQKPIKKFIPHDVQLLHTTIDRVEPETNLVHMTDGSALHYDI
                     LIIASGCNIAPEEVEGMKGELWYQDIFDFYSFKGAKALHEKLANWEGGKLVIHITEMP
                     IKCPVAPLEFAFLADDFFRHKGMRNEVEITYVTPLSGAFTKPFASKKLGHLIEDKGIK
                     LVTDFCVERIDNKTKTLHDYGGQEVNFDLLVTVPLNKGAEYVGRSGFGDDLNYVPTDK
                     FTLQTTIAPNIFAIGDATSLPASKAGSVAHFESEVLTQNILDFINDDPLSAKFDGHAN
                     CFVETGNHKALLIDFNYDHEPVDGTFPFNGVGPMQLMEESLMNHMGKLGFKWIYWNVL
                     MKGRTIPFIHPTMDTSGKHLEHETLNK"
     gene            3236..3547
                     /locus_tag="PLL28_07045"
     CDS             3236..3547
                     /locus_tag="PLL28_07045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HNF69117.1"
                     /translation="MTTLVNEKNVQFNEEGFMTDFSQWDKEVAEMIAAKEGIPVLTPK
                     HWEVINWLQEQHKSKNPLTIRKVGGSGITDIKEFYALFPNGPLKKAAKISGIPKPVSC
                     I"
     gene            3570..4091
                     /locus_tag="PLL28_07050"
     CDS             3570..4091
                     /locus_tag="PLL28_07050"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HNF69118.1"
                     /translation="MEDLLLNDAPVINEVDQKEPVRKMNFILSKAGLDSVYACFIMAN
                     GARMEGIEAEIFFTFFGLDAVNKKRMEHLHVATVGNPGMHMPTVLGAIPGMETFATNM
                     MKKQMEDLDLPTVPEFLEILEASGVEMYGCKLAADMFKLKKEDLFEGIKDIITVGDFY
                     AKASGKGSHIVFI"
     gene            complement(4204..5064)
                     /locus_tag="PLL28_07055"
     CDS             complement(4204..5064)
                     /locus_tag="PLL28_07055"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNF69119.1"
                     /translation="MRIDPSELTIPKLHQYILGSVSPRPISFASTVDLEGRPNLAPFS
                     FFTAVGSNPPMVVFSPARSGRDNTTKHTLDNVEATKEVVINIVNYPIVHQMSLASSPY
                     PKGVNEFIKSGLTPIPSEKVKPFRVKESIVSMECIVRDVIHTGDKGGAGNIVMCEIVL
                     MHIDDNILDADGRMDPFKMDLVARMGGEYYARIIPESIFVLPQPKTEVGIGVDSLPEF
                     ARNSHVLSGNDLGKLGTLQELPNEEALASAKTYLQAGMTGEDACVVAKQLISEGKTTE
                     ALALLVSFSE"
     gene            complement(5103..6383)
                     /gene="fahA"
                     /locus_tag="PLL28_07060"
     CDS             complement(5103..6383)
                     /gene="fahA"
                     /locus_tag="PLL28_07060"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HNF69120.1"
                     /translation="MKIAANDPKRKSWVPIPDGSDFPIQNLPFGIFVNDGKPAHVGVA
                     IGEYVMDLHALFRAGFLEEFDLTESMLENRFLNDLLHIGKKKMRALRDRISDLLLDSN
                     ESLQQHQEVYGSILIPMDQVEMLLPIQPGDYTDFYSSIVHATNVGSMFRPDNPLLPNW
                     KHIPIGYHGRSSSMIVSGTSFHRPLGQTIVKDGDPPIFGPTKQLDFELEMGFITFDGK
                     PLGQRISIEEADDYIFGMVIFNDWSARDIQRWEYVPLGPFLSKNFASSLSPWVVTLDA
                     LEPFRIAGEKQEPAVLPYLQYHGEKNLDINLQVFIKPDGSEAQLVSASNYKHMYWTME
                     QQLAHQTVNGCNVRAGDLYASGTISAPEATGFGSMLELAWRGTKPINMHDGTTRSFIL
                     DGDTVTIKAWSEKNGLRIGFGEVTAKVLPAIPFE"
     gene            6444..6926
                     /locus_tag="PLL28_07065"
     CDS             6444..6926
                     /locus_tag="PLL28_07065"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HNF69121.1"
                     /translation="MDIALLIIACVLMLGGIAGSFLPILPGPPLCFAGFLVVQFSIYA
                     SFSTEFLIIWAVVVVILGILEYYIPTWGTKKFGGTKSGQKGATVGTILGIFIPPQPLW
                     LILGPFVGAYLGELIHDARDNKKALRSAWGSFIGFLAGTFIKVVTVIVMSVFFIREVT
                     "
     gene            complement(6930..7283)
                     /locus_tag="PLL28_07070"
     CDS             complement(6930..7283)
                     /locus_tag="PLL28_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69122.1"
                     /translation="MAESNNWINTTLQEVVSLGELEVSESRLTVKNEDDARTLLIEMI
                     AFLIDHNFEKLLFILYRIDIDEQKVKLLLSKHLPDEAPVVIADLIIERQKQKEAFKAS
                     FEHTPPEAMDEDLLL"
     gene            complement(7270..8127)
                     /locus_tag="PLL28_07075"
     CDS             complement(7270..8127)
                     /locus_tag="PLL28_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HNF69123.1"
                     /translation="MQKHLTRRTSRTKSQQSLADHLDGLVVKYNNPDFIKGDPISLPH
                     KFTKLQDREIIGFWTAMLSWGQRKTIINKAEELLLLMHNSPYDFILHHKEDDLKRFAD
                     FKHRTFNYTDTLWFIYFLRSFYAEHNSLEEAFLCEGFREEVHVEKMLIHFHNLFFSDR
                     DAPQRTKKHIPTPASKSACKRLNMFLRWMVRKDNAGVDFGCWQQIQPSQLICPLDMHV
                     ERVARKLGLLTRKQTDWDAAVELTENLRMFDHSDPVKYDYALFGSGVMEKHVLDSEKK
                     AIINIHGRK"
     gene            complement(8090..8860)
                     /locus_tag="PLL28_07080"
     CDS             complement(8090..8860)
                     /locus_tag="PLL28_07080"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNF69124.1"
                     /translation="MDFQTLALLALAVGPAVAIMFFFYSKDKNDREPFGVLLVSFLTG
                     CFSVLPAIILEKALPHVVPGAQGESLISIAIFTFIIVAGSEEFSKYLFLRYYAYRKPS
                     FNEPFDGIVYAIMVGMGFATIENLLYVFGADTLGASWGTAGIRAVTAIPAHATFAAIM
                     GYFIGLAKFNRSHERRLMFYGFIGAVILHGFYDFFLFQNYKEGLVIGALISLIAGIIF
                     SIKAMRLHRANPFAIKPAAAQTKQHSDAETFDSPNKPN"
     gene            complement(8963..10102)
                     /locus_tag="PLL28_07085"
     CDS             complement(8963..10102)
                     /locus_tag="PLL28_07085"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HNF69125.1"
                     /translation="MKFNTKVIHAGVEPDPSTGAIMTPIFQTSTYVQNAPGDHKGYEY
                     ARTQNPTRHVLQQNLAALENGNFGICFSSGLAATDGIIKMFSEGDHIIANNDLYGGTY
                     RAFTKVYARFGMEFTFADLSDASQLEPLIKSSTKLIWVETPTNPMLRIVDIAAICAIA
                     KKHNILVCVDNTFASPYLQNPLDHGADIVIHSATKYLGGHSDVIHGAVIVKEQSLADQ
                     LYFIQNATGAVPGPQDCFLVLRGIKTLHLRVQRSCENAAVIAHWLKAHPKVDKVYWPG
                     FEDHANHAVAKKQMRMFGAMVSFTLKGDDFEEAKRILSSTHLFSLAESLGGVESLIGH
                     PASMTHASIPKEERLKNGLVDSLIRLSVGVEDVEDLVADLQKALG"
     gene            complement(10327..10689)
                     /locus_tag="PLL28_07090"
     CDS             complement(10327..10689)
                     /locus_tag="PLL28_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNF69126.1"
                     /translation="MNLPHSANKSIAVKLTFEFSLDIMHLCSQLDQGYKYSYSKQLFR
                     SGTGIGANVIEAQNAESKKDFLHKIKIAAKEAEETEYWLLLGNQSEEYPDMKDYLDKL
                     YAIKRVLNSIISTTKANL"
     gene            complement(11034..12275)
                     /locus_tag="PLL28_07095"
     CDS             complement(11034..12275)
                     /locus_tag="PLL28_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HNF69127.1"
                     /translation="MAIIRPFQGWRPQAAQAEQVASRPYDVLNSEEARVEAAGNDLSF
                     LHVCKPEIDLPEEVDHYDDAVYAKAVENWKRFQNNGTFVQDATRNMYAYRQIMNGHAQ
                     VGLVANSAISDYFDDIIKKHEFTRPEKENDRIRHMYDLQCHPEPVFLTYPDVADIDAI
                     MHKVVAGTPIYDFTADDGIQHTFWVISDAQDVAGLTQLFSSKVPFTYIADGHHRSASS
                     AKVGQRMKDANPNHNGEEEYNYFLSVIFPSSQLMIMDYNRVVKDSNGLSEDQLLDRIG
                     EKFSIEKHEGIYKPEGLHHFGLYINNHWYKLTAKQGTYNDNDPIDVLDVTILSNHLLD
                     PILGIKDQRTDKRIDFVGGIRGLGELEKRVNSGEMALAISLYPVSIQQLINIADSGNV
                     MPPKSTWFEPKLRSGLVVHTF"
     gene            12391..13257
                     /locus_tag="PLL28_07100"
     CDS             12391..13257
                     /locus_tag="PLL28_07100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69128.1"
                     /translation="MTKNYKLVTAQKLAAYSAMAAAITASMSDANAEVIYTDIDDVTI
                     GVGDIYQLDIDADGTADFMFGGGSTMSSGGSTWSFVSAFGYVTSYTVGNADNQIIGYT
                     GSFYNYGSALEDGALIGPDGPWLNYPSLSNSAVLASNFYGSTYGQFPGLGERFMGIKF
                     SSKGNLHYGWIRVVADIDPVYATIMDYAYETVNDVPIEAGSTISLVGVEELSAGTVNV
                     YAFNSSVRVVVNQDLTSPQIRITDLHGATVFTAPLNNRQATIDLKNFSTGNYVVSVLS
                     AEGTSNKQVFIN"
     gene            complement(13338..14258)
                     /locus_tag="PLL28_07105"
     CDS             complement(13338..14258)
                     /locus_tag="PLL28_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNF69129.1"
                     /translation="MGTILLATEKPFATEAVEKIRTVVTGGGHQLALLENYTEKQQLL
                     NAVADADALIVRSDIVDADVINAGNHLKIVVRAGAGYDNLDLKSASANNVVCMNTPGQ
                     NANAVAECAFGMMLFMARKQFSGSSGTELRGKNLGIVGYGNIGRYMAQIGRGFGMDVY
                     AYKRNMDRLAMEIDGVTPASSFEDVFEHCQYISLHLPSTAETKGVINYHLLRLMPKGA
                     MLVNTARVDVVNEDDLLKLMEERSDIQYVSDFIPKQESVFKEKFAKRIFYPAKKMGAQ
                     TEEANVNAGIAAAKQILDFLDKGIDTFRVN"
     gene            complement(14351..15418)
                     /gene="serC"
                     /locus_tag="PLL28_07110"
     CDS             complement(14351..15418)
                     /gene="serC"
                     /locus_tag="PLL28_07110"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNF69130.1"
                     /translation="MKVHNFNAGPAILPAPVISQAAQAVTEFNGLGMSLLEISHRSKD
                     FQAVVDEATALVKEIAGLDDSYKVLFLQGGASTQFGMVPMNLLDASETAGYCNTGTWA
                     KKAQKEAKQFGNVLELATSEPDNFTFIPKGYQIPDNLKYFHITSNNTIYGTQYHAYPK
                     SPVPYIIDMSSDIFSRNCDFSQFDLIYAGAQKNIGSSGVTVVILKESLLGKVNRTIPT
                     MMDYRTHIKENSLFNTPPVFSIYVTMLTLRWIKEQGLNIIESVNRSKAELLYNAIDSN
                     PLFKGTVAKEDRSWMNVNFIMENRDLEPEFLAFAKANNCVGLAGHRSVGGMRASLYNA
                     LGLDSVQHLVNLMNEFAAKKG"
     gene            15802..>16339
                     /locus_tag="PLL28_07115"
     rRNA            15802..>16339
                     /locus_tag="PLL28_07115"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         4818 a         3597 c         3095 g         4829 t
ORIGIN      
        1 ctttagataa agattttgaa tccgttatta agtcctgcgc gcagatcgaa cggaaaaatg
       61 atgagtctac ttggatcgat aattatttta tcgatgcata ttgtaaactc catcatcaag
      121 gacatgcaca ctctgtagaa gtttggcaga acgaaaaact cgttggaggt ttatatggca
      181 ttattcaagg cggcgttttt ttcggggaaa gcatgttcag tcgcgtaagt aatgcgagta
      241 aatatggctt tatttcactc gtagcgcatt tgcaaaaaca tcaattcgat tttatcgatt
      301 gtcagattta taacgatcac ctggcaagcc tgggagcaca taacatttcc agagaagaat
      361 atatgttgag attgcataat ggtttataca agaacgcaac ccctgttgta ccggaaacac
      421 tgtaatttac ggagccagtc gatgaatttt ccagctgcca tcgctgtgtt tgaggtattg
      481 taacctgtcg tgtaatcgat tacttcttcc ttgccagaat tcgaattctt caggtattaa
      541 aatataacct ccccaatgtt cgggacgctt taattcaccc tttaattgct gcgcgatgaa
      601 ttgctgttcc aaccattcac gaccttcaat tacctcactc tgtggcgata tcatggcacc
      661 cgcctgactg ccaacaggtc gtgaataaaa atatgcatca ctttctgtgg cgggaatttt
      721 ttccatcctc ccatttatgc gaacttgacg ttccatcgcc ccccaccaaa aattcataca
      781 tgcatatgga ttagctgcta attctttccc ttttctgcta ttataatttg tgaaaaaaat
      841 aaatcctgca gacacatatt cctttaacaa aacaacgcgc gaacttggtt taccctcatt
      901 acttaccgtc gataacacaa gggcgtttgc ttcgcttatt ccggctttta ttgcttcctc
      961 aaaccacaat ccaaattgct gcatcggttg tggataggca ttttgctcat cgaaaacacc
     1021 tgttgtgtaa tctgtgcgca tatcatgcaa ttttccttcg ttctgttcca ttgatatttg
     1081 aattaacctt gtttaacatg caaatcttac aaattatttc ataatccatc ataacttgcg
     1141 tcaccatgcg ttcttggctt atgcccttta cccgaatttc ggctattcta ttagtcctcg
     1201 tgtgctcact gaagcatggt gaggctcaac aatatgcgca attgagtggt atcattaccg
     1261 ataattccac cggatatgca gtgccatacg catctgtaac tgttcctgat gatatcagag
     1321 gggtgtctgc ctccgaaatt ggttttttta cgattgttgt caaacctttg gataccctgc
     1381 aattcagtgc attggggtat aaaccaaaac tatatgtcgt tccggatacc atgcacgatg
     1441 atttggtgtc catagcggtt tccctacagc gcgataccat agaactcgat tctttcgtcg
     1501 tttacccctg gccttcaagg gaagactttc gcgaagcgtt tttagcatat caggagacac
     1561 aaccctatgt gatgcagcca attccgggta tcaggggtaa agaattgatc gataccgtcc
     1621 ccaaaccccc ttcacccatc atgaacccaa tttctttctt ctacgatgaa gtggtgaaac
     1681 ccatccaatg gcgcaaaccc aaacgacaca tggtcgatga actccccgag tggaaataag
     1741 cactcaccac tcaccactca cgactcacga ctcacgactc acgactcacc cactcaccca
     1801 ctcacaactc accactcacc cactcacaac tcaccactca cccactctcc cactcgccac
     1861 tcaccaaaac ggtgacataa atcaatttcc gctgtatgaa acatcacctg ttatcgacct
     1921 ccgtcgggaa taattttgtg tcataaaata aacacaatga caagcaggaa cataatcatc
     1981 ttaggtgccg gatcagccgg aactatgatg gcgaaccatt tgcggggaaa actggataga
     2041 aatgagtgga atatcaccat cgttgatgaa gttgaaatcc actattatca acccggattc
     2101 ttattcctcc catttggtat ttacgatgaa catgagattc aaaaaccaat taaaaaattc
     2161 atccctcacg atgtgcaatt gctacacact accatcgatc gcgtagaacc tgaaactaat
     2221 ttagtacata tgacagatgg cagcgcgttg cactacgata ttctcattat cgcttcaggt
     2281 tgcaacatcg ctcccgaaga agttgaagga atgaaaggtg aactgtggta tcaagatatt
     2341 tttgatttct acagctttaa aggtgccaaa gcattgcatg aaaaattggc aaattgggaa
     2401 ggtggcaaat tagtgataca catcactgaa atgccgatta aatgccccgt agcaccactc
     2461 gaatttgcgt ttcttgccga tgatttcttt cgccataaag gaatgcgaaa tgaagttgaa
     2521 atcacttatg tgacccctct cagcggcgct ttcacaaaac catttgcttc aaaaaaactt
     2581 ggtcacctga ttgaagataa aggcattaaa ctggttaccg acttctgtgt tgagcgcatc
     2641 gacaataaaa caaaaacttt gcacgattac ggcggacaag aagtgaattt cgatttattg
     2701 gtaaccgtgc cattgaataa aggtgctgaa tatgtaggtc gaagtggttt tggcgatgat
     2761 ttgaattatg tgccaacaga taaattcaca ctccagacta ctatcgcacc aaacattttt
     2821 gccatcggcg atgctaccag tctaccggcc agtaaagccg gctcagttgc acactttgag
     2881 tctgaagtgc ttactcaaaa catcctcgat ttcattaatg atgatccatt gagtgcaaaa
     2941 tttgatggtc acgcaaattg cttcgttgaa accggtaatc ataaagcact attgatagat
     3001 ttcaattacg accacgaacc ggttgacgga acattcccat tcaatggtgt tggcccgatg
     3061 caattgatgg aagagtcact tatgaaccac atgggtaaac ttggtttcaa atggatttat
     3121 tggaatgtgc tcatgaaagg acgcactatt ccattcattc atccaaccat ggataccagc
     3181 ggaaaacacc tcgaacacga aactttaaat aaataacaac attaaacata ccattatgac
     3241 aacgcttgta aatgaaaaaa atgttcagtt caatgaagaa ggtttcatga ctgattttag
     3301 ccaatgggat aaagaagttg ccgaaatgat tgccgcaaaa gaaggcatcc cggttctcac
     3361 tcctaaacat tgggaagtaa ttaactggtt gcaggaacaa cataaaagca aaaatccatt
     3421 gaccattcgt aaagttggtg gaagtggcat aactgatatc aaagaatttt atgctttatt
     3481 cccaaatgga ccactgaaaa aagcagctaa aatttccggt ataccaaaac ctgtaagctg
     3541 tatttaataa ccgcatttaa aacattacta tggaagactt actgttaaat gatgctcctg
     3601 tgatcaacga ggttgatcaa aaggaacccg ttcgtaaaat gaacttcatc ctgtcaaaag
     3661 caggtttaga tagcgtttac gcttgtttca tcatggctaa cggcgctcgt atggaaggaa
     3721 ttgaagccga aattttcttc actttctttg gattggatgc cgtgaataaa aaaagaatgg
     3781 aacacttaca tgttgctact gtcggaaatc cgggaatgca catgcctaca gtactaggcg
     3841 ctattcccgg aatggaaacc ttcgcaacta atatgatgaa aaaacaaatg gaagatcttg
     3901 atctgccaac tgtacctgaa ttcctcgaaa tattagaagc ttccggagtt gaaatgtacg
     3961 gctgtaaact tgctgccgat atgtttaaac ttaaaaaaga agacctgttc gaaggcatca
     4021 aagatatcat taccgttggc gatttctacg caaaagccag cggcaagggc tcgcacattg
     4081 tgtttattta aaatagtatg tagctccgac cgaaaggtcg gggctctttt ttttaattat
     4141 gtgaaacttg tacgataggt agacacttgg taaatcggta gcgtaagtaa tttgctacaa
     4201 atctcattcc gaaaacgata ctaataatgc aagtgcctct gttgtctttc cttccgaaat
     4261 taattgttta gcaacaacac aggcatcttc tcccgtcatg cctgcttgca aatatgtttt
     4321 tgctgaggct aatgcttctt cgtttggtaa ttcctgtaat gttcccagct tacctaaatc
     4381 gttgcctgat aatacatgac tgtttcgtgc aaattcagga aggctatcaa cgccaatgcc
     4441 tacctctgtt ttaggttgtg gtaacacaaa tattgattcc ggaattattc ttgcataata
     4501 ttcacctccc atgcgggcta caaggtccat tttaaaagga tccatcctgc cgtcagcatc
     4561 cagaatattg tcgtcaatgt gcatcaacac aatttcacac ataacaatat ttcccgcgcc
     4621 acccttatcg cccgtatgaa tcacatctct aacaatacat tccatcgaaa caatgctttc
     4681 cttaaccctg aaaggtttta ctttttctga tggtattggc gttaatcccg atttaataaa
     4741 ttcattcact ccctttggat aaggagaact tgcaagactc atttgatgaa ctatcggata
     4801 atttacgata ttgataacaa cttccttagt ggcttcaaca ttatctagtg tgtgtttggt
     4861 ggtgttgtct ctgccgcttc ttgcagggga aaaaacaacc ataggcggat tgctccccac
     4921 agcggtaaaa aagctgaatg gtgcaagatt tggccttcct tccagatcga cagtgcttgc
     4981 aaaagaaatt ggccttggcg aaacagagcc taaaatatat tgatgcagtt tcggaatagt
     5041 caattccgat gggtcaattc tcatgaataa tgattattat taaatgatgc tatttattgt
     5101 tgttattcaa atggtattgc cggaagcact ttagcagtaa cttcaccaaa tccaatgcgt
     5161 aaaccatttt tttctgacca tgctttaatg gtaacagtgt cgccatctaa aataaaacta
     5221 cgagttgtcc catcatgcat gtttattggc tttgtgcctc tccatgccaa ttccagcata
     5281 ctgccaaatc ccgtagcctc gggagcactg atggtgccac tggcgtaaag gtcacctgca
     5341 cgcacattgc atccatttac cgtttgatgc gctaattgct gctccatggt ccaatacatg
     5401 tgcttataat tggatgcact cactaattga gcttcactgc catcaggctt aatgaaaact
     5461 tgtaagttga tatcaaggtt tttttcacca tgatattgca gatatggaag tacagcaggt
     5521 tcttgttttt ctcccgcaat gcgaaatggc tctaatgcat caagtgtaac aacccaaggc
     5581 gacaatgagc tggcaaaatt cttactcaga aacggtccca gtggaacata ttcccagcgt
     5641 tgaatatctc ttgcactcca atcattaaat atcaccatac caaaaatgta atcatccgcc
     5701 tcttcaatag aaatccgctg acctaatggt ttaccatcaa aggtgataaa ccccatttcc
     5761 aattcgaaat ctaactgctt tgtcggaccg aaaataggtg ggtctccgtc tttgacaatt
     5821 gtctgaccaa gcggtcggtg aaatgaagtg ccggaaacaa tcatggatga acttctgcca
     5881 tgatatccaa ttggaatatg tttccagttt ggcaataatg gattatcagg cctgaacatg
     5941 ctgccaacat ttgttgcatg cacaatgctc gagtaaaaat cagtgtaatc tcccggttga
     6001 atcggtagta acatctccac ctgatccatg ggaattaaaa tagaaccgta tacttcctga
     6061 tgttgctgca gcgattcatt gctatctaat aacaaatctg aaatgcgatc gcgcaaggca
     6121 cgcatttttt tcttgcctat atgcagaaga tcatttaaga atcgattttc caacatggat
     6181 tcggtaaggt caaattcttc aagaaatcct gcacgaaata atgcatgaag atccattaca
     6241 tactcgccaa tggcaacacc aacatgggca ggtttgccat cattcacaaa aatgccaaaa
     6301 ggcaaattct gtatgggaaa gtcgctgcca tcaggtatcg gaacccatga ttttcttttt
     6361 ggatcgtttg ctgcaatctt catattcatt aattaatttt acaaagttac aacaatgggg
     6421 tcgcatactt gttgatatag atcatggaca tcgcacttct tattattgcc tgtgtattga
     6481 tgctcggtgg tattgccgga agctttttac ccatattgcc cggtcctcca ttatgctttg
     6541 ccggattctt agtagtccaa ttcagcatct acgcctcgtt ttcaactgaa tttctcatca
     6601 tttgggctgt agtagtggtt atacttggta tactcgaata ttatattcca acctggggaa
     6661 cgaaaaaatt tggcggaaca aaatcaggtc aaaaaggtgc taccgttggc actatccttg
     6721 gcattttcat tccacctcaa ccattatggt taattctagg tccttttgta ggggcatacc
     6781 tgggtgaatt gatacatgat gcacgagata acaaaaaagc actccgaagc gcttggggtt
     6841 catttatcgg atttctggca ggtaccttca ttaaagtggt aaccgttatt gtaatgtcgg
     6901 tattttttat tcgtgaagtt acctgatcct tataataaca aatcctcatc cattgcctcg
     6961 ggaggagtat gctcaaacga agccttaaat gcctcttttt gcttttgtcg ctcaatgatc
     7021 aaatctgcaa ttacaacagg cgcttcatca ggcaggtgtt tagataatag aagtttaacc
     7081 ttctgctcat ctatgtcaat cctataaagg ataaataata atttttcaaa attatgatca
     7141 atcaaaaatg cgatcatttc gataagtaaa gtgcgcgcat catcctcgtt tttcacggtg
     7201 agcctgctct cagaaacttc caattcccct agacttacaa cttcttgcaa tgtggtattt
     7261 atccagttat tactttctgc catgtatgtt gatgattgct tttttctctg aatccaaaac
     7321 atgcttttcc atcacacctg acccaaataa ggcgtaatcg tatttcaccg gatctgaatg
     7381 atcaaacatg cgcaaatttt ctgttaactc aaccgccgca tcccaatcgg tttgcttgcg
     7441 ggtaagtaat cccaatttac gtgccacacg ttccacatgc atatcgagcg gacaaatcaa
     7501 ttggcttggc tgaatttgtt gccaacagcc gaaatccaca cccgcattgt ctttgcgaac
     7561 catccatcgc aaaaacatat tgagcctttt gcatgcactt ttggatgcag gggtaggaat
     7621 atgtttttta gtgcgctgag gtgcatcccg gtcagaaaag aacaaattgt ggaaatgaat
     7681 gagcattttt tctacatgaa cttcttcacg gaaaccttca cacaaaaacg cctcctccag
     7741 tgaattatgc tcagcataaa aggaccgtag gaaatatata aaccacagag tatccgtata
     7801 gttgaaagtg cgatgcttaa aatcggcaaa tctctttaaa tcgtcttctt tatgatgtaa
     7861 aataaaatca tagggactat tatgcattaa tagtagcagc tcttccgcct tattgataat
     7921 tgttttacgc tgaccccagc tgagcatggc agtccagaaa cctatgatct cccggtcttg
     7981 taatttggtg aatttgtgtg gaagcgaaat aggatcaccc ttgataaaat caggattgtt
     8041 atattttacg accaaaccat ctaaatgatc agcaagcgac tgctgcgatt tagttcggct
     8101 tgttcggcga gtcaaatgtt tctgcatctg aatgctgttt agtttgcgca gcagccggct
     8161 ttatggcgaa aggatttgca cggtgtaaac gcatagcttt aatgctgaaa attattccgg
     8221 caatgagcga tatcaaggcc ccgattacca gaccttcttt ataattctgg aataagaaaa
     8281 aatcataaaa tccgtgcaat atgaccgccc ctataaaacc ataaaacatc aatcggcgtt
     8341 catgtgagcg attgaatttt gccaatccta taaaataacc cataatcgca gcaaaagtag
     8401 catgcgcagg aatcgcagtt acagctctta tgcctgcagt tccccatgaa gcacctagtg
     8461 tatcggcacc aaaaacataa agtaaatttt caattgtcgc aaagcccatt cctaccataa
     8521 tagcataaac tattccatca aaaggctcat taaatgaagg cttgcgataa gcataatatc
     8581 gaagaaataa atatttactg aactcttcac ttccggcaac aataatgaag gtaaatatag
     8641 caattgaaat caatgattcg ccttgcgctc ccggtactac atgtggtaat gccttttcta
     8701 aaataatggc cggcaataca ctgaaacatc cggttaaaaa actcaccaac aacacaccaa
     8761 aaggctcccg gtcatttttg tcttttgaat aaaagaaaaa catgatcgca acagcgggac
     8821 caaccgctag tgccagtaat gcaagtgttt gaaaatccat ttattgtgtt tgatgtttgc
     8881 tgaaataaat atactgctcc cggactgaat gtaaagagca ataattccta ttaattcaat
     8941 acttattgta aatcaattgt attcaaccta atgctttttg caaatcggca acaagatcct
     9001 caacatcttc aacaccaaca ctcaacctga tcaatgaatc taccaatcca tttttcaatc
     9061 gctcctcctt tggaatggaa gcatgcgtca tacttgctgg atgtccgatt aagctttcaa
     9121 cacctcccaa gctttctgcc aaactgaaaa gatgcgttga cgaaagtatg cgtttcgcct
     9181 cctcaaaatc atcacctttg agcgtgaatg aaaccatagc accaaacatg cgcatttgtt
     9241 ttttggctac tgcgtgatta gcatgatctt caaatccggg ccaatatact ttatctactt
     9301 taggatgtgc tttcaaccaa tgggcaatta ctgcagcatt ttcacaactc cgctgaacgc
     9361 gcaagtgcaa tgttttgatt ccgcgtaata ctaagaaaca atcctgcgga ccaggaaccg
     9421 ctcctgttgc attctgaata aaatataatt gatcggcaag cgattgttcc tttacgatta
     9481 ctgcaccatg tattacatca ctatgtccgc ccaaatattt ggttgcggaa tgaattacta
     9541 tatcagcacc atgatctaga ggattttgca aatatggcga agcaaatgta ttatcaacgc
     9601 aaacaagtat attatgtttt tttgcaattg cacaaatggc agcaatatct acaatgcgca
     9661 acataggatt tgttggcgtt tctacccata tcaatttcgt gcttgattta atcagaggtt
     9721 cgagttgcga agcatcactg agatcggcaa aagtaaattc catgccaaag cgtgcatata
     9781 cttttgtaaa agctcggtat gtgccgccat acaaatcatt gttggcaata atatgatcac
     9841 cttcgctaaa cattttaatt attccatcag tggcagctaa tccactggaa aaacaaatgc
     9901 cgaaattgcc attttccaac gcggcaagat tttgttgcaa cacatgacgt gttggattct
     9961 gtgtccgcgc atattcatac cccttatgat ctcccggcgc attttgaaca tacgtagaag
    10021 tttggaaaat aggcgtcatg atcgcccccg tactcggatc cggctccaca ccggcatgaa
    10081 taacctttgt attaaatttc atattaatta gcaaatttga gaattagcaa gttagcaagt
    10141 ttgagagtta gcaagtttga gaattagcta gtttgagggt tagttggttt gaaagtttat
    10201 tttgaaactt ttgatttttc agtctgaatt tcatttattt gcttttgaaa tcagctagtt
    10261 tgctaattct caaaatgtaa ttaattgata cttggtgtgc acaaagatag ccattagaga
    10321 atttgattac aaattagctt tagtagtgga aatgatgcta tttagtaccc gtttgatggc
    10381 ataaagttta tcaagataat ccttcatatc agggtattcc tctgattggt tgccgagtaa
    10441 cagccaatat tcggtttctt cagcctcttt cgctgcaatt tttattttgt gcagaaaatc
    10501 ttttttgctt tcagcatttt gggcttcaat gacatttgca ccaatacctg tgcctgatcg
    10561 aaataattgt ttgctataag agtatttata cccctgatcc agttgagagc ataggtgcat
    10621 tatgtcaagt gaaaattcga acgttaattt gacagcgata cttttattag cagaatgtgg
    10681 aaggttcatt tgtttttaaa agtaaaccta tttcaatatt ctctgctcaa atttttcaaa
    10741 atccaccgta agcgcttacg tctctaaaat ttgtaaagat tggatgttca taaaaaattt
    10801 cagtgacatc ccccccctcg cactactctt aaattcccat actcctacac tcctacatca
    10861 cattcccaca cacccacaca cccacactct cacacaccca cacccacact ctcaatcacc
    10921 gacactctca cactccctca ctcccacgca ccttcaaact tgctaacttg ctaactctca
    10981 aacttgcaaa ctagaactag agttccaagg taagtatatc ccaaataggc taattaaaac
    11041 gtgtgcacaa ccagcccgct tctcaacttc ggttcaaacc aggttgattt cggtggcata
    11101 acgtttccac tatccgcaat attgattaat tgctgaatac ttacagggta taacgaaata
    11161 gcaagcgcca tttcgccact gttcacacgt ttttctaatt cacccaaacc gcgaatgccc
    11221 ccaacaaaat caatccgctt gtctgtgcgc tgatctttta ttccaagaat tggatcgaga
    11281 agatgattag ataaaatggt tacatctaaa acatcaattg ggtcattgtc attatatgtg
    11341 ccctgtttcg ctgtgagttt ataccagtga ttattgatgt ataatccgaa atgatgtaat
    11401 ccttcaggtt tatatatgcc ttcatgtttt tcaatagaaa acttttcacc tatgcgatca
    11461 agtaattgat cttcacttaa tccattgctg tcttttacaa cgcgattata atccatgatc
    11521 ataagctgac tgcttggaaa aatcacactc aaaaaataat tgtattcttc ttctccgttg
    11581 tgatttggat ttgcatcctt catgcgttga ccaacttttg cactggatgc acttctatgg
    11641 tgaccgtcgg caatgtatgt aaatggaact ttcgaagaaa ataattgtgt caaccccgct
    11701 acatcttgtg catcactaat tacccaaaaa gtatgttgaa tgccgtcatc agcagtaaaa
    11761 tcatagatcg gtgtacctgc cacaacttta tgcatgatcg catcgatatc tgcaacatca
    11821 ggataagtca gaaatacggg ctcagggtga cattgtaaat catacatgtg acgaatacgg
    11881 tcattttctt tttccggacg ggtgaattca tgcttcttaa taatgtcgtc aaagtaatca
    11941 gagatagcag aattggcaac caatcctacc tgcgcatgac cattcataat ctggcgatag
    12001 gcatacatgt tgcgggtggc atcctgaaca aaagtgccat tattttgaaa tcgcttccag
    12061 ttttctacag cctttgcata aacagcatcg tcataatgat ctacctcttc cggcaaatca
    12121 atctccggct tgcaaacatg caagaaactc aaatcatttc ctgcagcctc cacgcgggct
    12181 tcttcactgt tcaatacatc gtaggggcga ctagccacct gttcggcttg agctgcttgt
    12241 gggcgccagc cttggaaggg tcttatgatt gccatgtgca aattttgccg caaagatagg
    12301 gtatatggca attactattc cccttcccaa ttcggctccc aatttgcaca cttaaaaaaa
    12361 aactatattt ttaaggtata ataataacgt atgacaaaga actacaaatt agttacagct
    12421 caaaaattgg cagcgtattc tgcaatggct gccgcaataa cagcatcaat gagcgatgca
    12481 aatgctgagg taatctatac agatatcgat gacgtaacta tcggtgtcgg cgatatttac
    12541 caactggata tcgatgcaga tggcaccgct gatttcatgt ttggtggcgg ctctacaatg
    12601 agctccggag gctccacatg gtcgtttgtg tctgcatttg gctatgttac cagctacact
    12661 gttggcaatg ccgacaatca aattatcggc tacaccggaa gcttctataa ctatggaagt
    12721 gccctcgaag atggtgctct catcggccct gatggaccat ggctgaatta cccaagcctt
    12781 tctaatagtg ctgtgctggc atccaatttc tatggctcaa catacggaca gttcccgggt
    12841 ctcggagagc gattcatggg tatcaaattt tcatcgaaag gaaacctgca ctacggttgg
    12901 attcgtgtag tagctgatat cgatcccgtt tatgcaacca ttatggatta tgcatatgaa
    12961 acagtaaacg atgtgccaat tgaagccggt tctaccatta gccttgttgg ggtggaagaa
    13021 ttaagtgcag gcacggtaaa tgtgtatgct tttaattcat cagtaagggt agttgtcaac
    13081 caggatctta ctagtcctca gattcgcata accgacctcc acggtgctac cgtattcact
    13141 gcaccactta acaatcgtca ggctaccatc gacctgaaaa atttctctac cggaaattat
    13201 gtggtttctg tgctgtcggc agaaggaact tcaaacaaac aagtatttat caattgataa
    13261 aataaaacaa taaaaaagcg gagcctgata atcagtgctc cgctttttat tttgttggca
    13321 ataggaagta accctcatta atttactctg aaggtgtcaa ttcctttatc cagaaaatca
    13381 agaatttgtt tagcagctgc aatacctgca tttacatttg cttcctcagt ttgcgcaccc
    13441 atcttttttg caggatagaa aatccgtttc gcaaattttt ctttgaaaac agattcctgt
    13501 ttaggaataa aatcacttac gtattgaata tcgctgcgct cttccatcaa ctttaacaga
    13561 tcatcttcat ttacaacatc aacacgagca gtgttcacta acatggcacc tttaggcatc
    13621 aatcgcaaca ggtgataatt aattacaccc tttgtctccg cagttgacgg gagatgtaaa
    13681 ctgatgtact ggcaatgttc aaacacatct tcaaagctgc tcgcaggtgt tacaccgtca
    13741 atttccatcg ccaatcgatc catgttgcgt ttgtaggcat aaacatccat gccaaatccg
    13801 cgtccaatct gcgccatgta gcgaccaata tttccataac ccacaatacc caggttcttt
    13861 cctcgtaatt cagtacccga gctaccgcta aattgtttgc gcgccataaa taacatcatc
    13921 ccaaatgcgc attcagcaac agcatttgca ttttgtcccg gcgtattcat gcaaacaaca
    13981 ttatttgccg atgcagattt cagatcaaga ttatcatatc cggcccccgc gcgaacaaca
    14041 attttcagat gattgcctgc atttatgaca tcggcatcaa caatatcgct gcgcacaatc
    14101 aacgcatcag catctgcaac cgcatttagt aattgttgct tttcagtata gttctccaat
    14161 aaagcaagtt gatgaccgcc acctgttacc accgttctta ttttctcaac agcttctgtg
    14221 gcaaatggct tttcagtagc taataaaata gtacccatgt atgtttattt aattcaatga
    14281 tttactttca gaaattgagt tatgtcacct ggcaactttg atgaagaagt ttagttttag
    14341 caataaagca tcaacctttt ttcgctgcaa attcattcat caggtttacc agatgctgaa
    14401 cagaatcaag accaagagca ttatataatg aagcacgcat accacctacc gagcggtgac
    14461 ctgctaatcc aacgcaatta tttgctttag caaaagctag gaattcaggc tcaaggtcgc
    14521 gattttccat aataaagttc acgttcatcc atgagcggtc ttcttttgct accgtaccct
    14581 taaataatgg gttgctgtcg attgcattat ataataactc agctttcgag cggttaacag
    14641 attcaataat atttagtccc tgctctttta tccatcgcaa agtcagcatg gtaacataaa
    14701 tagaaaaaac cggtggtgta ttaaataacg agttctcctt aatatgcgtg cgataatcca
    14761 tcatagttgg aatcgtgcga ttcaccttac caagaagact ttctttcaaa ataaccacgg
    14821 ttacacctga agaaccgata tttttttgag cgccggcgta aatgaggtca aattggctga
    14881 aatcacaatt tctactgaag atatcagagc tcatatcaat tatatatggc accgggctct
    14941 ttggatatgc gtggtattgc gttccatata tggtattatt tgaggtaata tggaaatatt
    15001 taagattgtc cggaatctga taaccctttg gaataaaagt gaaattatct ggttcagaag
    15061 tagccagttc caacacattt ccaaactgtt tagcttcttt ctgagccttt ttcgcccatg
    15121 tacctgtatt acaatagcct gcagtttcag atgcatccag caagttcata ggcaccatac
    15181 cgaattgagt agaagcacca ccttgaagga atagtacttt ataagagtcg tccaaaccgg
    15241 caatttcctt caccaaagca gtagcttcat ccactacggc ttgaaaatct ttagatcgat
    15301 gagaaatttc gaggagcgac attccgaggc cgttaaactc agtcacagct tgagctgcct
    15361 ggcttattac cggtgccggg agtatggccg ggccggcatt aaaattgtgg actttcatat
    15421 tttttttatg gtttacttgt ttttgttgtt ttttccgcta cctttgcact cccatttcgt
    15481 tctttataca gggaaacaga gttcaaaacg cggctcaaaa atacgatttc cgccctttta
    15541 aaaaagtgat gaaagtcagt gaagggattt aggctgaaac gtaggaactg cgggggtttt
    15601 taaaaatctt taattttttt tgcccaaaaa tttgacaatg caataaaggt tgcattacct
    15661 ttgtgtccca tttcgaaaaa aatgtggccc cgaaagttgg ggtatacata taagttcttt
    15721 gaagtattgg agaaacaaca agtagcaagt tcaggtaaaa attaataatt actgaagtca
    15781 atttaatact tacatttttt aacaatggag agtttgatac tggctcagga tgaacgctag
    15841 cggcaggctt aatacatgca agtcgagggg taacaggtcc ttcgggatgc tgacgaccgg
    15901 caaacgggtg agtaacacgt atgcaatcta cctctaactg gggcacaact ttgggaaact
    15961 gaagctaatt ccccgtaaca atatgaaatc gcatggtttt gtatttataa ttccgatggt
    16021 tagagatgag cgtgcgtccg attagatagt tggcggggta acggcccacc aagtcaacga
    16081 tcggtagctg atgtgagagc atgatcagcc acacgggcac tgagacacgg gcccgactcc
    16141 tacgggaggc agcagtaggg aatattgggc aatggaggaa actctgaccc agccatgccg
    16201 cgtgaaggat gaatgtccta tggattgtaa acttcttttg ttagggacga aacccctgga
    16261 ttcatccggg gctgacggta cctaaagaat aagcaccggc taactccgtg ccagcagccg
    16321 cggtaatacg gagggtgca
//
LOCUS       DAOQHY010000066        16257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1681_length_16257_cov_4.745587, whole genome shotgun sequence.
ACCESSION   DAOQHY010000066 DAOQHY010000000
VERSION     DAOQHY010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16257
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1681_length_16257_cov_4.745587"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            772..847
                     /locus_tag="PLL28_07120"
     tRNA            772..847
                     /locus_tag="PLL28_07120"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:807..809,aa:His,seq:gtg)
     gene            complement(1048..1818)
                     /locus_tag="PLL28_07125"
     CDS             complement(1048..1818)
                     /locus_tag="PLL28_07125"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013352894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNF69131.1"
                     /translation="MENSSNNSRRSFLIKGTLAALSVSFLNSFRFPAAHAASAPSLAE
                     FNPEGGTFELPKLDYGYDALEPYIDARTMEIHYSKHHQAYVTKLNEALAKAPELKGRA
                     LPDLIRNINELPEAVRNAIRNHGGGHFNHSMFWKLMSPTAKDSKASAELEAAINAKWT
                     SMDNFKTEFSKSASTVFGSGWAWLIVDKENNLAITTTPNQDNPIMDVAALRGRPILGI
                     DVWEHAYYLKHQNKRVDYIADFLNVIDWNQVSKWYGER"
     gene            1924..4029
                     /locus_tag="PLL28_07130"
     CDS             1924..4029
                     /locus_tag="PLL28_07130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNF69132.1"
                     /translation="MQKSGENISLQVEGMTCSNCALGISRLIEQKGAHDIYVNFATGD
                     VRFTLTPEQKLSVFVDGINGLGYKVISGNAEHNERKKKLSDIEIKFLITLPFTFLLLL
                     SMVPGLSALHQPLVQFLLCLPVFAIGFYHFGKSALGSIRSGIMNMDVLIFTGSTAAFV
                     YSLYGWYSGNFMSMMFFETSASIITLVLLGNMMEHRSVQQTTTAIRALQKLQPETAKR
                     VHFDLMAGGDHIDEIPADQLQKNDVILVNTGDRIPTDGMVMHGSAWVDESMLTGESAP
                     VYKQKGDVVTGGTISVDGSIKAKVTALPANSVLASIIEMVRRAQVDKPKLQRLADRIS
                     AIFVPVVLSIALLTLLISHFAFHMSFENSLMHSIAVLVIACPCAMGLATPTAVMVGLG
                     RSAKNGILIKGAATAETFAQTQVIIFDKTGTLTEGDLKVSGMQLFDCDAEFLGSLIQG
                     LEVHSSHPIAQSLLRHFAGSSKMNFDNVTEIKGKGISGKDAIGIEYQIIAATADQQPF
                     DIVVLRNDKLIAGLQLEDSIKQDAAEAIAYFNERGIETILLSGDNAKKCAQVAEATGI
                     STVYAAKSPAEKLELVKQISSTRTVTMVGDGINDSPALESAHVGISMSNATQIAIQSA
                     QIILLNGHLQKLVDAHKLSRATLKTIRQNLFWAFFYNILAIPVAAIGLLSPIIAALSM
                     AFSDVFVIGNAILLRSKKI"
     gene            4035..5321
                     /locus_tag="PLL28_07135"
     CDS             4035..5321
                     /locus_tag="PLL28_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNF69133.1"
                     /translation="MRSPNSTQAIQIFQVARFGALLLASICLSKSGMSVHDIGQYETC
                     ILLAGTLSFFWINGTTHTYLSQARRGRSSSLNSSLFWSVVCTSILLIGGMLLFQKPLL
                     QLFKLEIDTRFFWLLIIFFVVNNLAFIVDYYLLAADNAQGLLRLSAFHLIVQTSLIAI
                     PAFIYHDFQSAFTGAIVFVSIKAAITIFLTSRNGGLALDLAVVRKFLSDASPLILSFF
                     LGGMAIYVDGIIVTQFYDKGTFATYQYGAREFPITLLLANAFSAAMVAHISNAPSDTE
                     KVLQGTSQLMRRIFPIVIILMALSYWLYPLVYNPQFKNSYIYFNIYLLLTISRLCFPN
                     AILLGHGNSKIILRAGMIEFLLNIIVSITLLKLIGIQGVAYGTVIALMANKSILIYHL
                     HKQGVPLRTYFPVRLASIYGSVLVAVFIFFTYFIHL"
     gene            5318..6865
                     /locus_tag="PLL28_07140"
     CDS             5318..6865
                     /locus_tag="PLL28_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69134.1"
                     /translation="MRSTQRQFILWGSLLALCAAMVVVWGYTFGHENSIQLIPFALHA
                     HDPSLYDQDFFVSHAAARFPNERSFFMLLLMPFGNNLEWPSFIIYVVCSLLLFRALYG
                     IAIHLLEDPWYAFGALMLLIFPLNFHTLGLNELFGRELSTSLLSDTACAWAIWHFLQH
                     KKWSPWLLLIFATLMHPLSGLQVFLLISATLFTQSVLSHRVSQFFRMYGLQIALYMFT
                     GGAFIIALQLGLDDTQWDDATFFQTFFVFRNAHHYIPTAFPHKDWVLLGPLYLITPFL
                     LYRQSKALFIFSLWIIGGCIAYSFCVLQLHSVTVATAQWFKSTMWLEWFMIIAIMLTG
                     KTLITNIKGRSYGPAIYALTVLVSVAWLLIVFPQYRFWKINTAYELPFYEVTTPEIDI
                     SRQVKERTAKSDFLIQPAGMDELKYYSERSSFVDYKALTHTKGFIKEWSRRFELVYDI
                     DPATSKVISFDAVRLADEHFRALTPEKLRELHQTYAISHIVTFADVQLPFALLYKNEK
                     YALYQLY"
     gene            6875..7651
                     /locus_tag="PLL28_07145"
     CDS             6875..7651
                     /locus_tag="PLL28_07145"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNF69135.1"
                     /translation="MHYGIANQSLIPVRKSADHASEQTTQLLFGELYQVHEVRDTWIR
                     IVTHFDQYPGWIHIKQHTELTTAESDKLLKAPVSVAAEVVQIISNNARSFPVLIGSTL
                     YDFDGMNFRVGKEKFVYAGQAVSADSGLIQGEHIRRFALKFLHAPYQWGGRSPFGIDC
                     SGFTQLVFKLYGISLPRDAYQQADSGKTLHFIHEAREGDLAFFDNDEGKITHVGILLG
                     NDQIIHASGCVRIDLIDHYGIFNRDEKKYSHKLRILKRIV"
     gene            7648..9114
                     /locus_tag="PLL28_07150"
     CDS             7648..9114
                     /locus_tag="PLL28_07150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNF69136.1"
                     /translation="MKILSEAQLHEWDAYTILEQDISSDMLMERAAIAFTNSLLPHLG
                     FNEKIGIVCGPGNNGGDGLCIARLLHRKGFHVHVVLYGANTSSDNQLNYHRAVKAGVR
                     IFLGENDIIPEQIQSSSLIIDALFGSGLTRPLSAINASVVEQLNALHARKFSVDIPSG
                     MMADIPSSGVVFRADHTFSFETPKLAFFFPENASVLGNWKILPIGLSESFLQKAETPF
                     HIIDHNTISSLLRQRDTFSHKGSNGHALIIAGSASTPGAAALCSFAALEAGAGLVTLC
                     SDIDYPEDPEVMITQRKNASRKIADPKINVIALGPGMGIDEEALAICKLVLQSAKQTL
                     VLDADALNILASNKELINMLPAGSILTPHPGEYARLFGAFQQWDELIIQLQQIAKSTG
                     CILLYKRAFTIVVLPDGNIWFNSTGNAGMATAGSGDVLTGMITALLAQGYNAEDAALL
                     GVYLHGLSGDIAMQYHHGQNLAAGDILDHIQDAYAFMM"
     gene            9202..9888
                     /locus_tag="PLL28_07155"
     CDS             9202..9888
                     /locus_tag="PLL28_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNF69137.1"
                     /translation="MKIVALTGAGISAESGIPTFRGAGGLWEGHRIEDVASPDGWYRD
                     RSLVLRFYNERRKGIKLAKPNRGHVALAELEKQHQLHIITQNIDDLHERAGASNILHL
                     HGEIFKARSERNAHITVKQEDDINLGDLATDGAQLRPHIVWFGEEVPMMEPAIQIVQT
                     ADILLIIGTSMVVYPAASLMHYTKTGIPVFVIDPNIPAITKQSNIHFIQEPAGTGVDT
                     FIREFLPIYL"
     gene            9888..10448
                     /locus_tag="PLL28_07160"
     CDS             9888..10448
                     /locus_tag="PLL28_07160"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNF69138.1"
                     /translation="MSQSENPSNPWTTLEKKVIYDNPWIHVEEHQVINAAGNPGIYGV
                     VSFKNYAIGIIPVDNEGNTWLIGQHRYPFDLYTWEIPEGGGSIKESPLENAKRELAEE
                     AGLIADKWELIQEMQVSNSVTNEYAFIFLAQDLHPTPPNPDEDEALVIKKLHITEAYT
                     MVDKGEILDSLTVVALLRARVLGIGM"
     gene            10538..11086
                     /locus_tag="PLL28_07165"
     CDS             10538..11086
                     /locus_tag="PLL28_07165"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNF69139.1"
                     /translation="MDENILDQHEVEQSSYQLAGFWDRFLASLIDGLIFIPVTGLAFY
                     NMMTLKILPLALILGLLFIIYKVYMEAARGATIGKRAMGIRVVTMDYQPINSNASLAR
                     NSLFALNAVVGLVNTVMLFNAINFENTTGFMDYITFQNANSLQLANLIGLVVFISGLW
                     IAFDKNKQGLHDKIGKTLVIKD"
     gene            complement(11087..12433)
                     /locus_tag="PLL28_07170"
     CDS             complement(11087..12433)
                     /locus_tag="PLL28_07170"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNF69140.1"
                     /translation="MQHILVLGAGKSATVLIDYLLDNAAEYQWTVTIGDLDEDLAKQK
                     IKAHPQGNAVYFNADDAALCQKLIGESDIVVSLLPAFVHPVVAKICVALGKHFVSASY
                     VSDAMRALHNEAVSKGLILMNEMGLDPGIDHMSAMKIIDHVRNMGAEITGFRSYTGGL
                     VAPESDNNPWNYKFTWNPRNVVLAGQGTAKFLSNHRDKYIPYHRLFASYDIMDVPGYG
                     KFEGYANRDSLSYRSVYGLEHTGTIIRGTLRNQGFCDAWNVFVQLGMTDDTYHLEDAH
                     KHNWSDFTDAFIPSGAGSVRERLQQYLAIDNEQIMHKLDWLGLFDNNPIFTQIPERTF
                     TPAQLLQMLLESKLSLDAGDKDMCVMLHVMDYSLHGKAYSLQSSMVAIGQDEVYTAMA
                     KTVGLPAAIAVKHILSGTWQHTGVVTPVHSDIYTPVLQELSDTFNIHFVETLQEFA"
     gene            12480..12959
                     /gene="cdd"
                     /locus_tag="PLL28_07175"
     CDS             12480..12959
                     /gene="cdd"
                     /locus_tag="PLL28_07175"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNF69141.1"
                     /translation="MDTISIQLQLTRHSGTDNFSVEEQMLIKAAHEALSSAYAPYSQF
                     QVGAALLLEDGSIVKGSNQENAAYPSGLCAERVAFFSAGSQHPGKKIMAAAVTTSYPM
                     EHPISPCGACRQVMAEYELKQQTPIKLLLCSHDGSVIVVNNSRDLLPLYFSGEWLKK"
     gene            complement(13039..>16257)
                     /locus_tag="PLL28_07180"
     CDS             complement(13039..>16257)
                     /locus_tag="PLL28_07180"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HNF69142.1"
                     /translation="AQSPACGEWDYLTYTYLYEHTGVLDSTLLSHPWFLANGNAPDTF
                     NYSSTPTFISVPSWEYYIVYDDTTSLTTAVTGISTTSTATPFDAVHPISRTQIIWTAA
                     ELTAAGLTAGDITGMDFDFSSASSSLNDLTIRIATTALTSFGANTFEGGLTTVYSGDF
                     LPGTGWSGVDFDTPFNWDGISNLIIDISYQNESTGSAATINAESTVDPMVLSYAGADK
                     YIEFHTPDQADVYGNPLSGLNDQVTISFWSMGDAPFQPQNGTTFEAITADGARVLNSH
                     TPWSDTNVYWDAGNDGGYDRINKAATAAEYEGNWVYWTFTKNTSTGSMKIYKNGVLWH
                     SGTGKIKPMTGIDHLRLGKGNWGGSESYAGFMDEFAVWNVEMPAADIAAYMYKDLDAT
                     HPYAANLQLYYQFNDGDGISEPDQSGMYDLGLIGATTHLAKATEIARNAQTSVTRPIV
                     RFEQGVFTSHIDSVLIVDAVANDPITLVYYDPADPTTATDTTLVWPTNYYVYNYNVDG
                     TVSDSTFIAATNTIYNTDLPYYSAPFEVINQYELARYITPYGIGLDLGEGFTWTYDVS
                     DYRPILHDSVHLAAGNWQELLDMELWFIEGTPPREPKKVQNLWYGFYSYGLTPSYDEQ
                     TPPKTVTISADAANSRVKIRVTGHGFGGTSNCSEFCAREHYLYVDGEQVWSKEVWRDD
                     CDLNPVYPQGGTWVYDRANWCPGAEVWTYDYELTPEVSAGNTYTFDYNAEAYTWNGAG
                     SVPTYVTSVQLVTYGEPNFTHDAALDAIIAPSDDDMQSRKNPVCSDPIVRITNTGSAT
                     LTSLDITYGIKDYDQDTYTWTGNLAFMESEEVTLPNFGWSPTGTAFIATVSNPNGVAD
                     EYVYNNSITTPLTMPPVYPSDIILDLKTNLASHENDLFLYDETDSIIFQRTTFTDNTT
                     YKDTLHLDDGCYRLYLYDYGEDGLSWWANSDGSGWFRTRNIDGSYIKNFGTDFGGLIY
                     QQFTVGNYTPVEEQFTQPSDMQVYPNPADAEVFIRVALPHLSDVQLKVYDITGNLIYA
                     ETQKRTQTEMFHLDVSTWSAGAYIVTAQSEDKVFNTKFVVEH"
BASE COUNT         4587 a         3516 c         3351 g         4803 t
ORIGIN      
        1 ttgggcataa aatccgtgta atcctttaat cctaataatc cgtgattcag acaattattg
       61 tgttcatgtg tttggacatg aaatccgtgt aatcctttaa tcctaataat ccgtgattca
      121 gacaattatt gtgttcatgt gtttggacat gaaatccgtg taatccttta atcctaataa
      181 tccgtgattc agacaattat tgtgttcatg tgtttggaca tgaaatccgt gtaatccttt
      241 aatcctaata atccgtgatt cagacaatta ttgtgtttat gtgttcgggc atgaaatccg
      301 tgtaatcctt taatcctaat aatccgtgat tcagacaata attgtgttcg gggtttgcat
      361 tttgttgcgc acttgcacat ggagcgcagc gacatcccct ctcagctgaa ttcccacccg
      421 cctgcggcga ggctggcatt ctcacattct cacattccca cccgcctgcg gcgaggctgg
      481 cattctcaca ttcccacatg gagcacagcg acatccgcct cagcaggact tacaatctta
      541 cacacttaca cacttacaca cttgcacacc tgtacacttg catacttgca cacttgcatt
      601 cttacacact tacacacttg cacacttgca cacttacaca cttgcacact tacacacttg
      661 cacacttaca cacttgcaca cttgcacact tacacacctg cacacttgca cactttaata
      721 cagcgcattt gtcatttttc caccaattca atatctttgc acctccaaaa cggtgattgt
      781 agctcagctg gttagagcat cggattgtgg ttccgagggt cgtgggttcg agtcccatca
      841 ttcaccctaa gaaggttcga cttcaccagt cgaacctttt gtttttttag atatcctaaa
      901 ttttcgccac tcacccccac gccaaaggca agccaaactc accctctgac cacccgccta
      961 ctcggcgagg taaactcacc cacccgccaa aggcgaggca agctcaccaa tctcctctct
     1021 cctctctcct ctctcctctc tcctttctca tctttcccca taccacttac tcacctgatt
     1081 ccaatcaatc acattgagga aatcggcaat ataatcgact cgtttgttct ggtgcttgag
     1141 gtagtaagca tgttcccaaa catcgattcc aagtatgggt cgaccgcgca gagcagcaac
     1201 atccataata ggattgtctt gattaggagt agtggtaata gccaggttat tttccttatc
     1261 aacaatcagc caagcccacc cggagccaaa cactgttgag gccgatttgc tgaattcagt
     1321 cttgaaattg tccattgaag tccatttggc gttgatcgcc gcttctaact cagcagaagc
     1381 ttttgaatct ttagcggttg gcgacatcaa tttccagaac atcgaatggt tgaaatgtcc
     1441 gcccccatgg ttgcgaatgg cattgcgcac ggcttcaggc agttcgttga tattcctgat
     1501 cagatcaggt agcgcgcgtc ctttgagctc aggagctttt gccagtgcct cgttgagttt
     1561 ggtaacataa gcttggtggt gctttgaata atgaatctcc atagtacgcg catcgatata
     1621 cggttccagt gcatcatagc cataatcgag tttcggaagc tcaaaagtgc cgccttccgg
     1681 gttgaactcc gctaatgacg gcgcggaagc cgcatgtgcc gctggaaagc ggaaactgtt
     1741 aagaaagcta acactcagtg ccgccagggt gccttttatt aaaaaggacc ggcgtgaatt
     1801 gtttgatgaa ttttccatat agtcttgtcg ttattgtcaa tgataagtaa aactaaacaa
     1861 ttggctgtga atacctgctc aagaagtact gttcacaaac cttaacttta caaacagatt
     1921 tacatgcaaa aatcgggcga gaatatatca ttacaggtag aagggatgac ctgctccaat
     1981 tgtgcgctag gcataagtcg gctaattgaa caaaaaggtg cacacgatat ctatgtcaat
     2041 tttgctacgg gtgatgttcg ctttaccctt actcctgagc aaaaactcag cgtttttgtt
     2101 gatggtatca atggattggg ctacaaagta atttccggga atgcagaaca caacgagcgg
     2161 aaaaaaaagt tgtcggatat agaaattaaa tttctcatca cccttccatt tacatttctt
     2221 ttattgttga gcatggttcc gggcttgtcg gcactccacc aacctctcgt gcaatttcta
     2281 ttgtgcctgc cggtttttgc catcggcttt tatcattttg ggaaaagtgc acttggctca
     2341 atacgttcag gtatcatgaa catggatgtg ctcattttta cgggaagcac cgctgctttt
     2401 gtttatagct tgtatggttg gtacagcggg aatttcatga gcatgatgtt ttttgaaacg
     2461 tcggcaagca taatcaccct ggtattattg ggaaatatga tggaacaccg cagtgtgcaa
     2521 cagacaacta ctgctattcg cgccctacaa aaattacaac ctgaaactgc taagcgtgtg
     2581 catttcgacc tgatggcagg tggagatcat atagatgaaa ttccggcgga tcaattgcaa
     2641 aaaaatgatg ttatactcgt caatacaggc gatcgcatcc ctactgatgg catggtcatg
     2701 catggcagtg catgggtgga tgaaagtatg ctcaccggag aaagcgcacc tgtttacaaa
     2761 caaaaaggtg atgtggtcac aggcggaaca atttccgtcg acggaagtat taaagcaaaa
     2821 gtaactgctc tgcctgcaaa ttcagtttta gcgtccatca tcgagatggt tcgtcgcgcg
     2881 caagttgata aaccaaaatt acaacgcctc gccgatcgta tctctgccat ttttgtgcct
     2941 gttgtattaa gtattgcctt gcttacgcta ttaatatcgc attttgcctt tcacatgagt
     3001 tttgaaaatt cacttatgca tagcatcgca gtgctggtga tagcttgtcc ttgcgcaatg
     3061 ggtcttgcca caccaaccgc tgttatggtt ggacttggac gcagtgcaaa aaatggaatt
     3121 ctcattaaag gtgccgctac ggcagagact tttgctcaaa cacaagtgat tatttttgac
     3181 aaaacaggaa cgctcactga aggcgaccta aaggtaagcg ggatgcagct tttcgactgt
     3241 gatgcagaat ttttaggaag tctgattcag ggtctggaag tgcattcatc tcacccaatt
     3301 gcacaatcac tgcttcgaca ttttgcaggc tcttcgaaaa tgaatttcga taatgtaaca
     3361 gaaatcaaag gcaaaggtat cagcggaaag gatgctatag gcatcgaata tcagatcatc
     3421 gcagcaactg cagatcaaca accgtttgat atagtggtat tgcgcaacga taaattaatt
     3481 gcaggattgc aattggaaga cagtattaaa caagatgctg ctgaagcaat tgcatatttt
     3541 aatgaacgag gtattgaaac aattttattg agtggtgata atgcaaaaaa atgcgctcag
     3601 gttgctgaag ctacaggaat ttccaccgta tatgcagcaa aatctcctgc agaaaaacta
     3661 gagctggtaa aacaaatcag ttcaaccaga acagtgacca tggtgggtga tggcatcaac
     3721 gactctcccg ctttggaatc tgcacatgtt ggaatttcca tgagtaatgc cacacagatt
     3781 gccattcaat cagcacagat tattctatta aatggtcatt tgcaaaaatt agttgatgca
     3841 cataaactta gtcgcgcaac attaaaaacg atacgccaaa atctattttg ggctttcttt
     3901 tataatatac ttgcaattcc ggtagcagcc atcggattat tgagccccat cattgccgca
     3961 ttgagcatgg cattcagtga tgtattcgtt attggaaatg caatactgct tcgctcaaaa
     4021 aagatatagt tcgaatgcgt tccccaaatt ccacacaagc catacagatc tttcaggtag
     4081 cacgtttcgg agcattgctg ctggcatcca tctgcttgtc gaaaagcgga atgagcgtgc
     4141 atgatatcgg tcaatatgaa acctgtatac tactggcagg aacgctaagt tttttttgga
     4201 tcaacggcac tacacatacc tacctttcac aggcacgcag aggtagatct tcatcattga
     4261 acagctcgtt gttctggtcg gtggtgtgca cttccatact attaattgga ggcatgctct
     4321 tatttcaaaa accgcttctg cagcttttca aactagaaat tgatacccgc ttcttttggt
     4381 tgttgatcat tttctttgtt gtaaataatc tggcatttat tgttgattac tatttgctgg
     4441 cggcggataa tgctcaggga ttactacgct tgagtgcgtt tcatttgata gtacaaacat
     4501 ctctgatcgc tataccggca tttatttatc atgactttca atcagcattt acaggtgcca
     4561 tcgtttttgt gtcaattaag gctgctatta caatttttct gacttcgcga aatggcggac
     4621 ttgctttaga tcttgcagtg gttcgcaaat ttctgagtga tgcatcacca ctcattctat
     4681 cctttttctt aggaggcatg gctatttacg ttgatggtat cattgtaaca cagttttatg
     4741 ataagggtac atttgctaca tatcaatacg gggccaggga atttccgatt acattattac
     4801 ttgccaatgc ttttagcgcc gccatggtag cacatatcag caatgccccc tccgatacgg
     4861 agaaagtatt acaaggcacc agccagctca tgcgcagaat atttcccatt gtaatcatac
     4921 tgatggcatt gagttattgg ttgtatcctt tggtttacaa tccgcaattt aaaaacagct
     4981 atatctattt caatatatat ttattactta ccatcagcag gctgtgtttt ccaaatgcta
     5041 tcttgttggg tcatggtaat tcaaagataa ttttgcgtgc cggtatgatt gaatttctgc
     5101 tgaatatcat agtaagtatt accttattaa aactgatcgg cattcaaggc gtggcttatg
     5161 gcaccgtgat cgccttgatg gcaaataaat caatacttat ttaccacctc cacaaacaag
     5221 gagtaccgtt gcgaacttat ttcccggtgc gtttggcgag catttatggt tcagtattag
     5281 tagcagtatt tattttcttc acctatttca ttcacctgtg agatcaacac aacgccaatt
     5341 tattctctgg ggcagtctgc ttgctctttg tgcagccatg gtagtcgttt ggggctatac
     5401 gtttggacat gaaaacagta tacaactgat accgtttgcc ttgcatgcac atgatccttc
     5461 tctatatgat caagattttt ttgtgagcca tgctgcagcc agatttccta atgagcgttc
     5521 tttctttatg ctgctgttaa tgccatttgg caataatttg gaatggcctt catttatcat
     5581 ttatgtagtc tgttcactgc tcctgttccg cgcactttac ggcattgcta ttcacttgct
     5641 ggaagacccg tggtatgcat ttggcgcact gatgttgttg attttcccat tgaattttca
     5701 tacactaggt ctaaatgaat tatttggaag ggagttgagc acctcattgc tgtctgatac
     5761 tgcttgcgcg tgggctattt ggcacttcct gcaacataaa aaatggtcgc catggctgtt
     5821 gttgatattt gccacactga tgcatccttt aagtggattg caggttttcc tgttgatcag
     5881 cgcaacatta tttacgcagt cggtattatc acacagagtt tcgcagttct ttcgaatgta
     5941 tggtcttcaa attgcgctgt atatgtttac cggaggtgca tttattatcg ctctgcagtt
     6001 gggattagac gatacacaat gggatgatgc tacgtttttt cagacatttt ttgtttttcg
     6061 caacgcacat cattatatac caacagcatt tccgcataag gattgggtgc tacttggacc
     6121 attgtatctc attactccct tcttattata ccgtcaatca aaagcactct ttatattttc
     6181 actttggatc atcggcgggt gcatagctta tagcttctgc gttttgcaat tacatagtgt
     6241 taccgttgca actgctcaat ggttcaaatc gaccatgtgg ttagaatggt ttatgatcat
     6301 agccatcatg cttacaggta aaaccctgat cacaaatatc aaaggtcgaa gctacggccc
     6361 ggcaatttac gcactgactg ttctggtaag tgttgcctgg ttattgatcg tatttccgca
     6421 gtatcgtttt tggaaaataa atactgccta cgaattaccc ttttatgaag taactacacc
     6481 ggaaatcgat atcagcagac aagtaaaaga gcgcacggca aagtctgatt ttctgataca
     6541 gcctgccggc atggacgaat taaaatatta tagtgaacgc agcagtttcg tagactacaa
     6601 agccctcaca cataccaaag gtttcatcaa agaatggtcg cgacgctttg aactggtgta
     6661 tgatattgat cccgccacca gcaaggtaat cagcttcgat gccgtaagac ttgccgatga
     6721 gcatttcagg gctctgactc ctgaaaagct gcgtgaactg catcagacct atgccatttc
     6781 acatatcgtt acctttgcag atgtgcaatt gccattcgca ctgttatata aaaatgaaaa
     6841 atacgcactg tatcaattat attaataccc tattatgcat tacggtatag cgaaccaaag
     6901 tctaattcct gtcagaaaat cggcagacca cgcatcggaa caaaccacac aattattgtt
     6961 tggcgaattg tatcaggtgc atgaagtccg cgatacctgg attcgcattg ttacacattt
     7021 cgatcaatat ccgggttgga ttcacatcaa acaacatacc gaacttacaa ctgcagaaag
     7081 tgataaatta ctgaaagcac cggtatccgt tgccgcagaa gttgttcaaa tcatttcaaa
     7141 caatgcacgt agttttccgg ttttaattgg cagcacatta tatgatttcg atggtatgaa
     7201 ttttcgtgta ggcaaagaaa aatttgttta tgccggtcaa gccgtcagtg ccgatagtgg
     7261 attgattcaa ggagaacata tccgacgatt tgccctcaag tttttacatg ctccctatca
     7321 atggggcgga agaagtcctt ttggaatcga ttgctccgga tttacacaac tcgtatttaa
     7381 attatatggc atttctttgc ctcgcgatgc ataccaacaa gccgatagcg gtaaaacctt
     7441 acactttatt catgaagcaa gagaaggcga tctcgcattt tttgataatg atgaaggtaa
     7501 gatcacgcat gtaggaattt tactcggcaa tgatcaaatt attcatgcat caggttgcgt
     7561 gcgcatagat ctcatcgatc attatggcat tttcaatcgc gacgaaaaga aatattcgca
     7621 caagcttcgc atcctcaagc gcattgtatg aaaatactat cggaagcaca attacacgaa
     7681 tgggatgctt acaccattct tgaacaagat atcagtagtg atatgttgat ggaacgcgct
     7741 gcaattgcat ttacgaattc cctgcttcca catcttggtt tcaacgaaaa aataggtatt
     7801 gtatgtggac ccggcaataa tggcggcgat ggtctttgca ttgcgcgatt gctgcataga
     7861 aaaggatttc atgtacatgt ggttttgtat ggcgcgaaca caagctccga taatcaactc
     7921 aattatcatc gcgcagttaa agcaggtgtg cggatttttt tgggggaaaa tgatattatt
     7981 cctgaacaga tacaatcctc atcgctcata atagatgctc ttttcggatc agggttaacg
     8041 aggcctctct ccgctataaa cgcttctgtc gttgaacaac tcaatgcact gcatgcgcga
     8101 aaattttcag tggatattcc ttcgggaatg atggctgata taccttcatc gggagtagtt
     8161 tttcgtgccg atcatacatt cagcttcgag actccaaaac ttgcattttt cttccctgaa
     8221 aacgcatcag tactcggcaa ttggaaaata cttcctatcg gtttatctga atcattttta
     8281 caaaaggcag aaactccatt tcatattatt gatcacaaca cgatcagttc actattacgt
     8341 cagcgcgata cattttcgca taaaggaagt aatggacatg cacttataat tgcaggaagt
     8401 gcctcaaccc cgggagccgc agccttgtgc agtttcgcag cgttggaagc cggtgccgga
     8461 ttggttaccc tatgcagcga tatcgattat ccggaagatc ctgaagtgat gatcacgcag
     8521 cgcaaaaacg ctagccggaa aattgctgac cctaaaataa atgttattgc gcttggtccg
     8581 gggatgggta tcgatgagga ggcattagct atttgcaaac tggttttaca atcggcaaaa
     8641 caaacacttg ttcttgacgc tgatgcatta aatattttag catcgaataa agaattaata
     8701 aacatgctgc ctgccggaag catattaaca ccacatcccg gagaatatgc cagattgttt
     8761 ggcgcatttc aacaatggga tgaattgatc attcaattgc agcaaatagc caaatcaacc
     8821 ggttgcatct tgttatataa acgcgcattt accattgtgg ttttacctga tggcaatata
     8881 tggttcaaca gtaccggaaa tgcaggaatg gcaactgccg gcagcggaga tgtacttaca
     8941 ggaatgataa cagctctgct tgctcaggga tacaacgctg aagatgctgc cttgctggga
     9001 gtttatctgc atggcctcag cggcgatata gctatgcaat atcaccatgg acaaaacctt
     9061 gctgccggag atatcttaga tcatattcag gatgcctatg catttatgat gtaaatgtgg
     9121 cagattattt gatctgaacc tgttccttca gaataaaata tatacatttc tcattttttt
     9181 gcttttattt atttttactc aatgaaaatt gtcgccttaa ccggagcagg tatcagtgcc
     9241 gagagtggta ttccaacatt caggggtgcc ggaggcttgt gggagggaca tcgaatagaa
     9301 gatgtagctt ctccagatgg atggtatcgc gacaggtcgc ttgttttgcg tttttataat
     9361 gaacgtcgca aaggaatcaa gcttgcaaaa cccaatcgcg gacatgtagc tttagcagag
     9421 ttggaaaaac aacatcagtt gcatatcatc actcaaaaca ttgacgactt gcatgagcgc
     9481 gcaggagcga gtaatatttt gcatttgcat ggagaaatat tcaaagcacg cagtgaacgc
     9541 aatgcgcata ttaccgtaaa acaggaagac gatatcaatc tcggtgatct tgccactgat
     9601 ggtgcgcaat tgcgaccaca tattgtatgg tttggtgaag aagttcctat gatggaacct
     9661 gctattcaaa tcgttcaaac tgccgatata ttattgatca tcggcacaag tatggttgtt
     9721 tatcctgccg catcactcat gcattatact aaaacaggaa taccggtttt tgttatcgat
     9781 ccgaatattc cggcaataac aaaacaatca aatattcatt tcattcaaga acctgccgga
     9841 acaggcgttg atacctttat tcgcgagttt ctgccaatat atttatgatg tcgcaatctg
     9901 aaaacccttc caatccttgg acaacactgg agaaaaaagt catctacgat aatccctgga
     9961 tacatgttga agagcatcaa gtgatcaatg ctgcaggaaa tcccggcatt tatggtgttg
    10021 tttcgtttaa gaattacgct attggtataa tacctgtaga taatgaaggc aatacctggc
    10081 tcatcgggca acatcgatat ccttttgatt tgtatacctg ggaaattccg gaaggtggcg
    10141 gttcgataaa agaatcaccg ctcgaaaatg ccaaacgtga actcgccgag gaagcaggcc
    10201 tgattgccga caaatgggaa ttgatacagg aaatgcaagt gagcaattct gtaacaaatg
    10261 aatatgcatt tatttttctt gcacaagacc tccatcccac tccaccaaat ccggatgaag
    10321 acgaagccct ggtaatcaaa aaactacata ttacagaagc ttacaccatg gtcgacaaag
    10381 gtgaaatcct cgattccctt accgttgtag ccttgttgcg ggcaagggtg ttgggtatag
    10441 ggatgtagat tttcttgtta aaaaatgttc atccacaaga cctcatttgc atggtccgca
    10501 atatttcatt attattgaga aacaaaacac aattaccatg gacgaaaata tacttgacca
    10561 acacgaagta gaacaatcct cttaccaatt agcaggtttc tgggatagat ttctggcatc
    10621 attgattgat ggattgattt tcatacctgt aacaggtttg gctttctata atatgatgac
    10681 gctaaaaata ttgccattgg cattaattct tggattgctt ttcatcatat ataaggtgta
    10741 catggaagct gcacgaggtg caacaattgg caaacgcgcc atgggtattc gcgtagtaac
    10801 aatggattat cagcctatta attctaatgc ttcgcttgcc agaaattctt tatttgccct
    10861 gaatgccgta gtaggtttgg ttaatacggt tatgttgttc aatgccatca attttgaaaa
    10921 cacaacaggc tttatggatt acattacttt ccaaaatgcc aacagccttc agcttgcaaa
    10981 tttgattgga ttggtagtat ttatttccgg gttatggatc gccttcgata aaaacaaaca
    11041 aggcttacac gacaaaatcg gtaagacgct tgtaattaaa gattaatcag gcgaattcct
    11101 gtagcgtttc tacgaagtgg atattaaatg tatcagacaa ttcttgtaac acaggagtat
    11161 aaatatcgct gtgtaccgga gtcaccacac cagtatgttg ccatgttccc gagagaatat
    11221 gttttacggc aatcgcagcc ggtagtccta cagttttagc cattgccgta tatacttcat
    11281 cctgacctat tgccaccata cttgattgca gtgagtaggc cttaccatgc aaggaataat
    11341 ccataacatg tagcatcaca cacatatctt tatcaccggc gtccagcgat aatttgcttt
    11401 cgagtaacat ttgcaaaagt tgcgcaggag tgaatgtgcg ttcaggaatt tgagtgaaaa
    11461 ttgggttgtt atcaaataat cccagccaat ccagtttgtg catgatttgc tcattatcga
    11521 tagcaagata ttgctgcaaa cgttcgcgaa ctgatccggc tccggaagga ataaatgcat
    11581 cagtaaaatc agaccagttg tgtttatgag cgtcttcgag atgataggta tcatcagtca
    11641 ttcccaattg gacgaataca ttccatgcat cacaaaatcc ttgattgcgc aaagttcctc
    11701 gaataatggt tccggtatgc tctaagccat aaacgcttcg ataagataga ctatcgcgat
    11761 tcgcataacc ttcaaatttt ccatagccgg gcacatccat aatgtcgtat gaagcaaata
    11821 atcggtgata tggaatatac ttatcgcgat gattgctcag aaattttgcc gtcccctgcc
    11881 ctgccagcac cacatttctt ggattccagg taaatttata attccaggga ttattatcac
    11941 tttcaggtgc aacgagacca ccggtatatg agcgaaatcc tgtaatctca gcacccatat
    12001 tccgcacatg atcaattatt ttcatggcac tcatgtgatc gatgccggga tctaaaccca
    12061 tttcattcat taatatcaat cccttagaaa cagcctcatt atgcaacgca cgcatagcat
    12121 cgctgacata ggatgcagat acaaagtgtt ttcctaaggc gacacagatt ttagcaacaa
    12181 caggatgcac aaatgccggc aataatgaga ctacgatatc agactcacca atgagttttt
    12241 gacataatgc ggcatcatct gcattgaaat acacagcatt tccctgcgga tgtgctttga
    12301 ttttctgctt tgccaaatcc tcatcaagat cgccaattgt cactgtccat tgatattcgg
    12361 ctgcattgtc gagcagataa tcgatgagca ctgttgccga cttcccggct cctaaaacaa
    12421 gtatatgctg cataattgag ccgtaaaatt actactttca cccatagaat caggtgacca
    12481 tggataccat cagcatacaa ttacagctta ccagacatag tggaaccgac aatttcagtg
    12541 tcgaggaaca aatgctcatt aaggctgcac atgaggcttt gagttcagct tatgcaccct
    12601 attcgcaatt tcaggtaggt gccgcattgt tattggagga tggcagtata gtgaaaggaa
    12661 gcaatcagga aaatgcagca tatccatcag gattatgtgc tgagcgtgtt gctttcttca
    12721 gtgccggaag ccaacatccg gggaaaaaaa taatggccgc tgcagtaact acctcttacc
    12781 cgatggaaca tccgatttct ccttgtggcg cttgccgaca ggtaatggcg gaatacgaat
    12841 tgaagcaaca aaccccaata aaactcttgt tatgcagtca tgatggttct gtgatagtag
    12901 taaacaactc acgcgatttg cttccactct atttttcagg cgagtggttg aagaaataaa
    12961 tcggcatatt gcaaaacaaa aaatggcagg aattattaac tcctaccatt ttattataag
    13021 atttatttaa tctatttctt agtgttcaac aacaaacttg gtgttaaaca ctttgtcttc
    13081 cgattgtgct gtaactatgt atgctcctgc cgaccatgtg ctcacatcca ggtggaacat
    13141 ttcagtttgc gtgcgttttt gtgtttcagc atatatcagg tttcctgtga tatcatacac
    13201 tttcaattgc acgtcggaaa gatgtggtag tgccacgcga ataaacactt ctgcatccgc
    13261 aggatttgga tatacctgca tgtcggatgg ttgagtgaat tgctcttcaa ccggagtata
    13321 attgcctacc gtaaattgct gatatatcaa tccaccgaaa tcagtgccga aattttttat
    13381 atatgagccg tcgatattgc gtgtgcggaa ccatccgctg ccatcactgt ttgcccacca
    13441 gcttaaacca tcttcaccat aatcatataa atataaccgg taacaaccat catctaaatg
    13501 caaggtatcc ttgtatgtag tgttatcggt gaaggttgta cgttggaaaa ttatgctgtc
    13561 ggtctcgtca taaaggaaca aatcgttttc atgagaagca agatttgttt ttaaatccaa
    13621 aataatatca ctgggataaa caggtggcat tgtcaatggt gtagtgatag agttgttata
    13681 tacatattca tcggcaacac catttggatt actaacggtt gcaataaatg ctgttcctgt
    13741 tggcgaccat ccgaaatttg gcaaggtcac ttcttcactt tccataaagg ccaggttgcc
    13801 ggtccaggta taggtatcct ggtcgtaatc tttaattcca taagtgatat caagtgatgt
    13861 cagcgttgca gatcccgtat tggtaatgcg cacaatcgga tcactgcaca ccggattttt
    13921 tcgtgattgc atgtcatcat cagatggtgc gataatggca tcgagcgctg catcatgtgt
    13981 gaagtttggt tcgccatagg tgacaagttg aactgaagtt acatatgtag gaacacttcc
    14041 cgccccattc caggtatatg cttccgcatt ataatcaaaa gtataggtat ttccggctga
    14101 aacttcaggt gtcaattcat aatcataagt ccacacttca gctcccgggc accagtttgc
    14161 gcgatcgtaa acccatgttc caccctgagg ataaaccgga ttcaaatcac aatcgtcgcg
    14221 ccatacttct ttgctccata cctgttctcc atcaacatat aaataatgtt cacgcgcaca
    14281 gaattcagaa caattagaag tacctccaaa gccatgtcct gtaacgcgga tctttacccg
    14341 tgaattcgca gcatctgcgg aaatggttac tgtttttgga ggtgtttgtt catcgtatga
    14401 tggcgtaagt ccataactgt aaaatccata ccataaattt tgaacttttt taggctcacg
    14461 aggtggcgtt ccttcaataa accacaattc catatctaat aattcctgcc agttacctgc
    14521 agcaagatgt acagaatcat gtaatatcgg gcggtaatcg ctgacatcat aagtccaggt
    14581 aaatccttca ccaaggtcaa gtcctattcc ataaggagta atatatcgcg caagctcata
    14641 ttgattaatt acttcaaacg gagcactgta ataaggtaaa tcagtattgt agatagtatt
    14701 tgttgcagcg ataaatgttg aatcacttac ggttccgtca acattataat tgtaaacata
    14761 ataatttgtc ggccatacta atgtagtatc tgttgcagta gttggatcag caggatcgta
    14821 atacacaaga gtgatgggat cattggcaac agcatcaaca attaacaccg agtcaatatg
    14881 agaagtaaat actccttgct cgaaacgcac tattggtcgg gttacggagg tctgcgcatt
    14941 tcgtgcaatt tccgttgctt ttgcaagatg cgtggttgca cctatcaatc cgagatcgta
    15001 catacctgat tgatcgggct cggaaattcc atcgccatca ttaaactgat aatacaattg
    15061 aaggttagct gcatatggat gtgtggcatc aagatcttta tacatgtaag ctgcgatatc
    15121 tgctgcaggc atttcaacat tccaaacggc aaattcatcc ataaatccgg cataagactc
    15181 agatccaccc caatttcctt ttcccaaacg caaatgatcg attcctgtca tcggtttaat
    15241 tttccctgta cctgaatgcc agagcacgcc atttttatag attttcatgc ttccggttga
    15301 ggtgtttttg gtaaatgtcc aatataccca attgccttca tactcagccg ctgtagcagc
    15361 tttattgata cgatcataac ctccgtcgtt tccggcatcc caatatacat tggtatcgct
    15421 ccaaggggtg tgactattta atacacgcgc gccatcagct gttatagctt cgaaggtagt
    15481 accgttctgt ggctgaaatg gagcatcgcc catactccaa aatgagatgg taacctgatc
    15541 atttaatccg gacaatggat ttccataaac atcggcctga tcgggtgtat gaaattctat
    15601 gtatttatcg gcaccggcat agctcagcac cattgggtct acagtgcttt cagcgtttat
    15661 tgttgcagcg cttcccgttg actcattttg ataagaaata tcgatgatca gatttgatat
    15721 accatcccaa ttaaagggcg tatcaaaatc aacgcctgac cagccggttc cgggtaaaaa
    15781 gtctcctgaa taaactgtag tcaatccacc ctcgaaagta ttcgccccaa atgatgttaa
    15841 agctgttgtc gcgatgcgaa tggtaaggtc attcaaagat gaagaagcag agctgaaatc
    15901 aaaatccatc cctgtgatat ctccggctgt aagtcctgca gcagtaagtt cagctgccgt
    15961 ccatataatt tgtgttctgg aaatcgggtg cactgcatca aatggcgttg ccgtagatgt
    16021 agtcgatatt ccggttaccg ccgttgtaag cgaggtggta tcgtcataca ctatataata
    16081 ttcccacgat ggaacagaaa tgaatgttgg cgttgaagaa taattgaaag tatctggagc
    16141 attgccattc gcaagaaacc acggatgcga tagcagcgtg ctgtcgagta ctcctgtgtg
    16201 ttcatataag taagtataag taagataatc ccattcgcca catgcaggac tttgtgc
//
LOCUS       DAOQHY010000067        16227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1686_length_16227_cov_6.144076, whole genome shotgun sequence.
ACCESSION   DAOQHY010000067 DAOQHY010000000
VERSION     DAOQHY010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16227
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1686_length_16227_cov_6.144076"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..2140
                     /locus_tag="PLL28_07185"
     CDS             71..2140
                     /locus_tag="PLL28_07185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69143.1"
                     /translation="MKKLLLLTLAALPIVGWSQCTTTNATGCHCLDGSTECDLLPDIT
                     ASYDLLAETDETVETPGELRLSVGTPNIGHGPLRVVATDYYVCGGDTIYSPGGLTTCP
                     DGSSPKQIINQRIYHKSGSDMSYWERAAGTMTYHPDHGHFHTDNWGNYTLRKPVDGVD
                     DPTEWPIVGYGTKMGFCLMDLANCATPSNYGYCREDDGTVITNDIENYGLGGGGYNCV
                     VTNQGISVGYLDIYDYYLDGMSITIPEGVCNGDYYIVVEIDPNLNYWEESDNNNVIAV
                     PITLTEQPETIDLVHIAHSAGTELTTGTLTVCATESVELSVSPIGTDYLWSNGATTST
                     ITITEPGTYSCFITRECGDVYSDTINVEIVTSVEPVIDPSPVVCIGDATTLTATGDGI
                     INWYNAPIGGSMLGTGTTLITAALTENTTFYAENVNEILSAIDAFVGQTNHEGSNYST
                     GSPYNGYEVFDAIEDVTINSVKVYTDYPGDRLIELRNAAGTVMASSLVNIPSGTTVID
                     LDFDVPAGSNYRLGTNDAQNTTTFGDIAPRLKRSTSGTSYPYTVDGLISITNSSFDVT
                     RWYYFYDWHVTGNAVFSCPSERAAMAVEVKECTAIGELSSLNSFSVYPNPANGVFTVD
                     LNTQSVKDLAVSVCNATGQEVYGTVMSYVNGAITIPVDIRNAASGMYLVKVTSEGKSV
                     SKTIIVE"
     gene            complement(2359..3168)
                     /locus_tag="PLL28_07190"
     CDS             complement(2359..3168)
                     /locus_tag="PLL28_07190"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNF69144.1"
                     /translation="MKKILTLMCFSAVIGTVSAQQGLHFGAQGGFNSVWIMNQNNYGF
                     QELDYTRTFGYLAGASFGYNWDQTIGFQVDLNYVKVGQHYFDVSKDFCNIDANGDNKP
                     EKVDTYRYVDLNYIQLPIMFKYTSPRSKKQVVSVHILAGPTLGILAGADMYYEADTLA
                     LYNDEVQLIPGDSIDYMVPEFAQTAATEAASDYFSKIDIGFQAEIGADIYVNKNLYIS
                     PGLRVYGGLTDINSKPTRERRPDLETVSYEGASRNIYGGLTLGIHYMLPSK"
     gene            complement(3274..4221)
                     /locus_tag="PLL28_07195"
     CDS             complement(3274..4221)
                     /locus_tag="PLL28_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HNF69145.1"
                     /translation="MNTIPTVNFADFISDDADRKARFVQELGKAYQEVGFVAVQNHGI
                     DQQLVDGFYNAVESFFAMPDDIKSKYEIAGLAGQRGFTSFGKEHAKQSTAGDLKEFWQ
                     IGQTVEDGAALSEHYPENVRITEIAEFNELGYQLYRNFENTGKYLLRSLALFLGLEET
                     YFDKPIHNGNSILRAIYYPPITSEPKSAIRAEQHEDINLITLLVGASADGLEVMDTAG
                     KWIPVKTAPTEIVVNVGDMLQRLTNNVLRSTTHRVVNPPREKWHTARYSIPFFLHPVS
                     EMPLDCLENCVTAHNPLNYSPITAGEYLDERLREIGLKK"
     gene            complement(4586..6121)
                     /locus_tag="PLL28_07200"
     CDS             complement(4586..6121)
                     /locus_tag="PLL28_07200"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNF69146.1"
                     /translation="MAIEQFQYIRSLQAYEGREVTIKGWVSNKRDSKGLVFLIMRDGS
                     GFAQCVVDAAKVSEEQFEAAKRLTMESSVCLTGTVVKDERQVGGYEIQVSAVEIIQIA
                     QEYPIAKKEHGVEFLVENRHLWLRSRKQWAMMRVRNRVIFAIHSFFQQNDFLQMDAPI
                     FTGNACEGTSTLFETDFYGRPAYLSQSGQLYGEALAMAHGKIYTFGPTFRAEKSKTRR
                     HLSEFWMIEPEMAFYNLDMNMDLIEQFLKYVVDDVITHCASELETLERNTAFLRNAVT
                     QKFPRIHYDDAVAMIRGEKEVDGKNSIKLLEADLEGIKLRIAEIAKEIAEREVKIEGG
                     SLKQGEINFNKNKIDTLKNESKELEERASNIPQWLESARNFQHGNDFGGSDETVLTRL
                     FSCPIMVYNWPAAVKAFYMKRDPNDPRYVKGVDVLAPEGYGEIVGGAEREDNYEMLLE
                     RIQHEQLPVEAFDWYLDLRKYGTVPHAGFGLGLERMVMWISGGQHIREAIPFPRFYGR
                     LEP"
     gene            6284..7768
                     /gene="rpoN"
                     /locus_tag="PLL28_07205"
     CDS             6284..7768
                     /gene="rpoN"
                     /locus_tag="PLL28_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNF69147.1"
                     /translation="MLNQRLSQKLLQKLSPQQIQLMKLLQVPTDQLEQRIKEELEANP
                     ALEEGDREEQDASSLEEVFREEEKGEEGTGEGEENRENEELDLDDYINEDDVADYKTR
                     DENYGDDDEDNKTTPYAVTDTFHENLSKQLGMCELDERQEKIAQQILGSIDDDGYLRR
                     EISAIVDDLAFSQNVMTEENEIIDLLDLIQGFDPPGVGARDLRECLLLQLERKTQTKA
                     IKLAIHIIENYFEEFTKKHYEKLARHMRVDEDDLKPVITEITRLNPKPGGSAKEDGGS
                     MYQYIIPDFTIMNNNGELELKLNSRNAPELRVSRDFMDMMVEYKRGQQKDKKQKEAIL
                     FIKQKIDAAKWFIDAIKQRQHTLWSTMYEIMVHQYDFFLTGDETQLNPMILKDIAEKT
                     GLDISTVSRVANSKYVQTEFGTYALKYFFSESLSTESGEEVSTREVKKILSELIEGED
                     KKNPLSDQELMEDLRKRGYNIARRTVAKYREQLDISVARLRKEL"
     gene            7765..8382
                     /locus_tag="PLL28_07210"
     CDS             7765..8382
                     /locus_tag="PLL28_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69148.1"
                     /translation="MIKKIFGHFFSILFLPLLLPLFGALFVIYSDPYGFPDQGANILM
                     LIRVGLLTFLFPAITIFLLVRLKFVQNVTVYDRQERTIPYIACGFFYIWAFYVFYKEG
                     FNSHITFILLGATIAVFIDFLINILVVKVSMHTTGAGGLVAFMLVLFPYAQINVLPVL
                     LASIVIAGCVGAARLALKAHTEREVYLGYLTGFFSFMVASNFIHL"
     gene            complement(8369..9175)
                     /locus_tag="PLL28_07215"
     CDS             complement(8369..9175)
                     /locus_tag="PLL28_07215"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF69149.1"
                     /translation="MNKVFAIVILLASSLCLKANPDSTSTEGINNIDTTTFVYANVMY
                     EKGQYATSIDIYNRLLEQEGPSATLYYNIGNCYYKTGQLGEAVLNYERALFLDPGNDD
                     ATYNLSLANARIRDRMEPRTTSIFIIWWHAVSRIMGAQAWGILAIVAMWLALMGWAAY
                     LLPQFRAYQRIGFFTAIIAFSISILCTIGHFGRKNYDAGQTFAIVMSPSAVIKSEPSE
                     TSTNLALLHEGFKLKLIEADSSWSKIEMPDGVIGWMHRKDYAAINPFTGE"
     gene            complement(9172..11037)
                     /locus_tag="PLL28_07220"
     CDS             complement(9172..11037)
                     /locus_tag="PLL28_07220"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNF69150.1"
                     /translation="MRRCNVLIFMCLVTMACILPESLHAQVSFTATVNKNKVSVNERF
                     QFIITLNNGSNPENFRPPSFTDFTVLGGPNQSTSVQMFNNTVQQSMSFSYILQPKKTG
                     KFQIGAAYIKVKDKTYSSQPIVIEVTDAPPATAGAQNNNSGSNNGSSGSASGDIEQYL
                     RENLLIKTEISDQDVYEGESFTVVLKLCIPDDGTIAGLNGYRITKTPAYDGFYVEDAD
                     LRNEQFKAENINGKNYKTVIIKKTTLTPQQSGNLVIDPTSLDAGVVVRVKKQKKNSGD
                     PFQDMLNDFFSDPFSSGSREFTASLSSKTTGIKIKPLPANAPADFNGAVGKFTMSSQI
                     NSTSTKTDEPLTYRITINGTGNLQLFNAPELDLPAGWETYDPKVSESSGTKIFEYLLI
                     PRSPGDFTIPAFTWSYFDPSVKKYQTLQTDAYPVKVTAGPGYNPSANNYAANKEDVEL
                     LGKDIRYIDKDSPKFSSGTKDMKSGFLLALILTPAIGGAGLFVFTRRQKAKMSDVGAM
                     RAVKANATAKKRLAAAGKFLQQNNSRGFYDETVRALWGYLSDKLHIPQNTLSKENIEQ
                     VLLNKHVSASTIDQFIQLLDTCEFSLYAPNIQHSDLELTFNSASSLISTLENELR"
     gene            complement(11037..11732)
                     /locus_tag="PLL28_07225"
     CDS             complement(11037..11732)
                     /locus_tag="PLL28_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF69151.1"
                     /translation="MMKKNNIKHLLAIAFIFAVRTLTAQNANELIKEGNAKYDKQDYD
                     GARENYQEAVKASPESVEGNFNLGNSLYMQQQYDAAAEQFSKAAEGTKDPEIRSQAYH
                     NLGNTLLEKKDYSGSIDAYKKSLRDKPEDADTKYNLSYAQQMLKKQQQQQQQKQDQQK
                     QQQQQQQKQKQQQQDNNPQNNNQDKQPEPNTPDKKDLDRILKSLNEDDKAVQDKVNKQ
                     KARPAGGDPEKDW"
     gene            complement(11716..12765)
                     /locus_tag="PLL28_07230"
     CDS             complement(11716..12765)
                     /locus_tag="PLL28_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNF69152.1"
                     /translation="MSFERPDILYLLILLPALLLMYWWYLRWRTARIRKIGDSELIRD
                     LMFRDSDKRRWIKYSLQLLGIAFLILGIANLRQGSKEQKVSGESAEVIICFDVSNSML
                     AEDVKPNRLTQAKFTASELIEKLAANKVGLVVFAGNSYVQMPLTRDARAAMMYLNTIN
                     TTSVPTQGTAIGSAIETAMLSFENGGEKDDKKGRAIIIITDGESHDDNAEEMAKKAAS
                     EGIKIITLGVGTSTGGPIPLRKNNNIEGFKKDRSGNIVLTKLNEQMLNDLATDADGVY
                     MNLAEGKRVIQEVYKTIDELDKTVDGDFEFTEYVNHFQLFLGIGLFLLILEFLMTDKK
                     PKWVEKIDLFDDEKK"
     gene            complement(12765..13763)
                     /locus_tag="PLL28_07235"
     CDS             complement(12765..13763)
                     /locus_tag="PLL28_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNF69153.1"
                     /translation="MSDIQFADPWFLLLLAGIPVYVYFRSKIERRKFVAMRMSTTSGI
                     PKAPQGKAKFMSVLLILRLLAFALIVIAMARPQTSYSNSRMYSNGIDIMLALDVSPSM
                     YAIDFKPNRMEAAKTAAKEFIDSRPNDRIGLVVFAGEAFTQCPSTLDHNLLKEQVDNA
                     DNYYLKDGTAIGDGLFMAVNRLADSTNISQKVIILLTDGVRVGGKFSPLDAANGAKEL
                     NMRVYTIGIGTNTNSPIPVFDKNGRHIFDLDPRISFDAPMLKNVASITGGSYFEADSK
                     EKLTDIYQQIDKIEKTKFSMDISRRYDEHFYWFAVGGLICILLEVILSKTIFRTLT"
     gene            complement(13769..14701)
                     /locus_tag="PLL28_07240"
     CDS             complement(13769..14701)
                     /locus_tag="PLL28_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69154.1"
                     /translation="MMKKLIVILLIIITCNQLQGQIISAHANTDKPSYSIGDYISVDI
                     TISGPASFKYYFPDETTISTYDIISSMPIDTQRSDDRVILTKHIIYSIYEGGDVYFPQ
                     VAINYQSTSDTAQHQILTDSIPLHIEAIAVDTTLAIKPIKSVLDVTLRNHKWILWVGI
                     ITGLIIIGGLIWYFFLRRKNIGPIAVPVPLKSLHERMTERLQQLQSKQLWQKGQLKTY
                     YSELTDILRDYLEERYHMHAHESTSDEIVEQLERHPDTRAHASNIRYLLNLADMAKFA
                     KSQPLADENTRAFDHVLNFIEQTREIITETPKQE"
     gene            complement(14702..15577)
                     /locus_tag="PLL28_07245"
     CDS             complement(14702..15577)
                     /locus_tag="PLL28_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNF69155.1"
                     /translation="MDKATLLKKVRLIEIKTKGISQNIFSGEYHSAFKGRGMSFSEVR
                     EYAYGDDIRNIEWNVTARMNHPYIKIFEEERELTVMLLIDISASSLFGTQKQTKKALI
                     TEIAAVLSFSAINNNDKVGVILFSDRIEKFIPPKKGKSHILRIISDLLEVKAEGSGTD
                     IALALEYFNNVVKKKSIAFMFSDFFDMHYEKPLMVAARKHDVIGVHIHDKREEDLPDA
                     GIIRMFDPESRNIQWVDSSNKTVRASYKKYFNTNLQYFKNTFLRSGADTISIHSELSY
                     INALHAFFKKRISMR"
     gene            complement(15611..>16227)
                     /locus_tag="PLL28_07250"
     CDS             complement(15611..>16227)
                     /locus_tag="PLL28_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNF69156.1"
                     /translation="VQSALLEAMQERQVTIGEHTYKLEEPFLVLATQNPIEQEGTYPL
                     PEAQVDRFMLKVVIHYPEKEEEKMIIRQNVSGAIQQIKQVVKPEDILKARDLVREVYM
                     DEKIENYILDIVFATRDPEAYKLGKLKHLIAYGGSPRASINLALASKAYAFIKRRGYV
                     IPEDVRAVCRDVMRHRIGLTYEAEAENITAEQIISELLNVVEVP"
BASE COUNT         4462 a         3448 c         3265 g         5052 t
ORIGIN      
        1 cccccacatt tcaaaagata tttttatctt taatatccct tttggcataa aggggcttag
       61 acaacttatt atgaaaaaat tactacttct caccttggcc gctctgccta tcgtcggctg
      121 gagccagtgt accacaacaa atgcaacagg ttgccactgc cttgatggtt caaccgaatg
      181 cgacctttta ccggacatta ctgcatctta cgatctgctt gcagagactg acgaaacagt
      241 tgaaaccccc ggagaattgc gcctttcagt tggaactcca aacatcggac atggtccttt
      301 gcgtgtggtt gccaccgatt attatgtttg tggtggtgat accatttact caccgggggg
      361 gctaaccact tgccctgatg gatcctctcc aaaacagatc atcaatcagc gtatttacca
      421 taaatcagga tctgatatgt catactggga gcgcgctgcc ggtactatga cctatcaccc
      481 tgaccacggg cactttcata ccgataactg gggtaattat acattgcgta aacctgtcga
      541 cggtgtagat gatcccacag aatggccaat tgttggttat ggtaccaaaa tgggattctg
      601 tctgatggat ctcgccaact gtgctactcc atcaaactat ggctattgtc gcgaagatga
      661 tggcacggtt attacaaatg atattgaaaa ttatggcctc ggtggaggtg gatataactg
      721 cgtagttacc aatcaaggca tctctgtagg ctatctcgat atttatgatt attacctcga
      781 tggtatgagc attaccattc ccgaaggtgt ttgtaatggc gattattata ttgttgttga
      841 aattgatccc aacctcaatt attgggaaga aagtgataac aataacgtaa ttgctgtgcc
      901 tattacatta actgaacaac ctgaaacaat tgaccttgtg catatcgcac attctgccgg
      961 aactgaactt actaccggca ctttaactgt atgcgctact gaatcagttg agttatctgt
     1021 ttcaccaatc ggtactgatt acttatggtc aaatggtgct actacaagca caattactat
     1081 cactgaaccc ggaacatatt cttgcttcat tacacgtgaa tgtggtgatg tttactccga
     1141 tactattaat gttgaaattg taacatcagt tgaacctgtt attgatccat cacctgttgt
     1201 atgtatcggt gatgcaacaa ctttaactgc aaccggagat ggtatcatta attggtataa
     1261 tgcacctatt ggcggttcta tgcttggcac cggaaccaca ttgatcactg ctgcattgac
     1321 agaaaatact accttctatg ctgaaaatgt aaatgagata ttatctgcaa tagatgcttt
     1381 tgtgggacaa acaaatcatg aaggttctaa ttatagcaca ggttcaccat ataatggtta
     1441 tgaagttttc gatgctattg aagatgtaac tataaactcc gttaaagtat ataccgatta
     1501 tcccggagat cgcttaatcg aattgcgcaa tgcagcagga actgtaatgg catcaagtct
     1561 tgtaaatatt ccttcaggaa caacagttat tgatttagat tttgatgtac ctgccggaag
     1621 taattatcgc ttaggtacca acgacgccca aaatacaaca acctttggcg atatcgctcc
     1681 taggttgaag cgcagtactt caggaacttc atatccatat actgttgatg gtttgatatc
     1741 gatcacaaac agtagcttcg atgtaacacg ctggtattat ttctacgatt ggcatgtaac
     1801 aggaaatgca gtattcagtt gcccaagtga gcgtgctgca atggctgttg aagtgaaaga
     1861 gtgcacagct atcggcgagt tgtcatctct caatagcttt agcgtttatc caaatccggc
     1921 aaatggcgta tttacggttg atttaaatac tcaatctgta aaagaccttg ccgtatctgt
     1981 ttgtaatgca acaggtcaag aagtttacgg tacagttatg tcgtacgtaa atggcgccat
     2041 cactattcct gttgatatca ggaatgctgc ttcaggaatg tatcttgtaa aagttacttc
     2101 tgaaggtaag agcgtgagca aaacaattat tgttgaataa tatcaatatc aaataatgaa
     2161 agccgcttca taacgaggcg gcttttttat ttgtcgcatg atgcctttgg tatttttatg
     2221 aagcaagata ttatagttgc ttactttgat ttattattga aagattactg tttgttaaat
     2281 cgctttttga aattaattat agcacaaaaa aaatcccgtc actaaacgca acgggatcat
     2341 taaatatgat tatttaaatt atttacttgg caacatatag tgaataccta gtgttaatcc
     2401 accataaata ttgcgagaag caccttcata gctaaccgtt tccaaatcag ggcgacgttc
     2461 gcgtgtaggt tttgaattga tgtcggtaag tccgccataa acgcgtaaac ccggactgat
     2521 atataaattt ttatttacat aaatatcagc gccaatttct gcctgaaagc cgatatcgat
     2581 tttagaaaag taatcactgg ctgcttcggt tgctgctgtt tgagcaaatt cgggtaccat
     2641 gtaatcaatt gaatcacctg gtattaattg cacttcatca ttatacaaag caagtgtatc
     2701 tgcttcgtaa tacatatcag cgccggctag tatacctagt gtaggtcctg caagaatatg
     2761 cacagacaca acctgctttt ttgatcttgg cgatgtgtat ttgaacatta tcggcaattg
     2821 aatataattt aagtcaacat atcgataagt gtcaactttt tcaggtttat tatcaccatt
     2881 tgcatcgata ttgcaaaagt ccttagacac gtcaaaatag tgctgtccaa ctttaacata
     2941 gtttaggtct acttgaaaac cgatagtttg atcccaatta taaccgaatg acgcaccggc
     3001 aaggtaaccg aaagttcggg tgtaatcgag ttcctggaat ccgtagttgt tctgattcat
     3061 gatccataca ctgttgaaac cgccctgcgc accaaagtgc aatccttgtt gggcagatac
     3121 ggtgccaata acagcgctga aacacattag ggttaaaatc tttttcataa caggttttat
     3181 ttggatatta gggtatagac aacaaaggta acagaaatcc gcaccataaa acaggtgtta
     3241 ctggcaatct tggaatgata tatcccgtga aacctatttt ttaagcccga tttcacgaag
     3301 tcgctcatcc agatattctc ctgccgtgat tgggctataa ttgagcggat tgtgcgcagt
     3361 aacgcagttt tccaggcagt cgaggggcat ttcggacacc ggatgtaaaa agaaaggaat
     3421 ggaataacgg gcagtatgcc atttttcgcg aggaggattc acgacgcggt gtgtagtgga
     3481 tcttaggaca ttattagtga gcctttgcag catatcgcca acattcacca caatctctgt
     3541 aggagccgtt tttacaggaa tccattttcc tgcggtatcc atcacctcaa gtccatcggc
     3601 actggcgcca actaataatg ttatcaggtt aatgtcttca tgctgctcag cacgtatggc
     3661 agattttggc tcagaagtaa tgggcggata atatattgca cgaagtatcg agttgccatt
     3721 gtgtattggc ttgtcaaaat aagtttcttc caaccctaaa aacaacgcca atgaccgcaa
     3781 taggtattta ccggtatttt cgaaattgcg atataactga tatcccagct cattaaattc
     3841 agcaatttca gtaattcgta cattttcagg gtaatgttca ctcaatgcag ccccgtcttc
     3901 aaccgtttgg ccaatctgcc agaattcttt caaatcgccg gctgtgctct gtttggcatg
     3961 ttctttacca aatgacgtga atccgcgttg tcccgctaat cccgcaattt catacttgct
     4021 ttttatgtcg tcaggcatag cgaaaaagga ctctactgca ttgtaaaatc catcaactaa
     4081 ttgttgatct ataccatgat tttgtacagc gacaaagcca acttcctgat acgcttttcc
     4141 aagctcctgc acaaatcgcg ctttgcgatc ggcatcatct gaaataaaat ctgcaaagtt
     4201 tacggtgggt atggtgttca tgctgccggt atttataatt tcgaagatac gatgttggga
     4261 ggagaaagga aaaaggagag aggagaaagg agaaaggaga gggtgagttt acctcgactt
     4321 tgcgccttag cctcttagcc gtcattgcgc gaaaccctat gaatgtcaag accccttcta
     4381 cctcaaatcc tcaattcttc aaaccttcga atcttcaatt cttcaaacct tcgaatcttc
     4441 aattcttcaa accttcaaat cttcaattct tcaaaccttc gaatcttcaa ttcttcaaac
     4501 cttcaaatct tcaattcttc aaaccttcga atcttcaaat ctttaaacct tcgaatcttc
     4561 aattcttcac acctcaaccc cttcatcacg gctccaaacg tccgtagaaa cgtgggaatg
     4621 gaatagcttc gcgaatatgc tgaccacctg agatccacat caccatacgc tcaagtccta
     4681 atccgaaacc tgcatgcggc acagtgccat atttacggag atctaaatac caatcgaatg
     4741 cttctacagg aagttgctca tgttgaatgc gttccaataa catctcgtaa ttatcttcgc
     4801 gctcggcacc accaacaatt tcaccatatc cttccggtgc cagcacgtcg acacctttta
     4861 cataacgcgg atcgttaggg tcgcgcttca tatagaaagc ttttaccgca gcaggccagt
     4921 tgtaaaccat gataggacaa gagaacaggc gcgtgagtac agtttcatca cttccaccaa
     4981 aatcattgcc gtgctgaaaa ttgcgtgccg attccaacca ttgaggaata ttacttgcgc
     5041 gttcttcaag ctccttactt tcgtttttga gcgtatctat tttgtttttg ttgaagttga
     5101 tctcgccctg ttttaatgag ccaccttcaa ttttcacttc gcgctctgca atctcttttg
     5161 caatttctgc aatgcgcaat ttaattcctt caagatcagc ctcaagtaat ttaatactgt
     5221 tttttccatc aacttctttt tcacctctga tcatggcaac ggcgtcatcg taatgtatgc
     5281 gcgggaattt ttgtgttacg gcattgcgca ggaatgctgt attgcgttct aatgtctcca
     5341 attcagatgc acaatgtgta atcacatcgt caacaacata tttcagaaac tgctcgatca
     5401 aatccatatt catatccaga ttgtagaatg ccatctccgg ttctatcatc cagaattctg
     5461 ataagtgtcg acgcgttttt gatttttcag ctctgaaagt agggccgaac gtataaatct
     5521 taccatgggc catcgccagg gcttcgccat ataattgtcc gctctgactc aaatatgcag
     5581 ggcgaccata aaaatctgtt tcaaacaagg ttgaagtgcc ttcgcaggca tttcctgtaa
     5641 aaatgggggc atccatctgc aggaagtcat tttgctggaa gaagctatga atagcgaaga
     5701 tcacgcggtt gcgcacgcgc atcatggccc attgcttgcg actgcgaagc cagaggtgac
     5761 ggttttctac taaaaactcc acaccatgct cttttttagc gatgggatac tcctgtgcta
     5821 tctgaattat ctctacagcg cttacctgaa tctcatagcc accaacctga cgctcgtctt
     5881 ttacaacagt gcctgtcaga caaacggaac tctccatagt taggcgtttt gcggcttcaa
     5941 actgctcttc actcactttt gctgcgtcta caacacactg cgcaaaacca ctgccatcgc
     6001 gcatgataag gaataccaaa cctttgctgt cgcgcttgtt gctcacccat cccttgatgg
     6061 ttacctcacg gccttcatat gcctgcagcg atcgtatata ttgaaattgt tctattgcca
     6121 tatctgttta ctttgagggc acaaaattac gatgaatttt ccacgcatac agtctttgac
     6181 ccttgtactc gttaataaat aaaagccata catacaatgg gaaaaatgtt gtcgccacaa
     6241 gtaaaatcgg cgtaaatttg acccgaaata catcaatcag cgtatgttga accaaaggtt
     6301 aagtcaaaag ctcctgcaga aactctctcc gcagcaaatt cagctcatga agctgttgca
     6361 ggtgcctacc gaccagttgg agcaacgcat caaggaagaa ctggaagcca atccggcatt
     6421 ggaagaggga gaccgcgagg aacaagacgc ctccagcctt gaagaggtat ttcgcgagga
     6481 ggaaaaaggt gaagaaggca ccggtgaggg tgaagaaaat cgcgaaaatg aggagctcga
     6541 cctcgacgac tatattaatg aagacgatgt agcggattat aaaacgcgtg atgagaatta
     6601 tggcgacgat gatgaggaca acaagactac accatacgcc gttaccgata ccttccatga
     6661 aaatttaagt aaacaattag gcatgtgcga gcttgatgaa cgtcaggaga agatcgcgca
     6721 gcaaattttg ggcagcatag atgatgatgg ttatttgcgt cgcgagattt cggcaattgt
     6781 cgatgatctt gctttttcgc aaaacgtaat gacggaggaa aatgagatta ttgacctctt
     6841 ggatcttatc cagggatttg atcctcccgg tgtaggtgca cgtgatttga gagaatgtct
     6901 gttgctgcaa ttggaaagaa aaactcaaac caaagccatt aaactggcca ttcatatcat
     6961 cgagaattat ttcgaggaat tcaccaagaa acactacgaa aaattggcgc gacacatgcg
     7021 tgttgatgaa gatgatttga agcctgttat taccgagatc accagattaa atcccaaacc
     7081 gggtggcagt gcgaaggaag atggtggaag catgtaccaa tacattattc ccgattttac
     7141 catcatgaac aataacggcg aacttgaatt aaaactcaat agccgcaatg ctcctgaact
     7201 gcgcgtaagc cgcgatttta tggatatgat ggtggagtat aagcgcggtc agcaaaaaga
     7261 taaaaaacaa aaagaagcca tcctcttcat caagcaaaaa attgatgcgg cgaaatggtt
     7321 tattgatgct atcaagcaac gacaacatac actgtggagc accatgtacg aaatcatggt
     7381 acatcaatat gatttcttcc ttaccggaga tgaaacgcaa ttgaatccga tgatcctgaa
     7441 agatattgcc gagaaaacag gattggatat ttcaacagta agccgtgttg caaatagcaa
     7501 atatgttcaa acagaattcg gaacctacgc tttgaaatat ttcttcagtg aatctttgtc
     7561 gaccgaaagt ggcgaagaag tgagtacacg cgaggttaaa aagatattgt ctgaattgat
     7621 cgaaggtgag gataaaaaga atccactttc agatcaggaa ttgatggaag acctccgcaa
     7681 acgcggctat aatattgccc gacgcacggt agccaagtat cgcgaacaat tagacatttc
     7741 cgttgcccgt ttaagaaagg aattatgata aaaaaaatat tcgggcattt cttttccatt
     7801 ttattcctgc cgctattgct gcctttgttc ggagccttgt ttgttattta ttcagatcct
     7861 tacggttttc cggatcaagg cgccaatatt ttaatgctta tcagggtggg attgctcacg
     7921 tttttgttcc cggcaataac tattttctta cttgtcagac tgaaattcgt tcagaatgta
     7981 acagtctatg accgacaaga gcgcacgata ccatatatcg cctgcggatt tttctatata
     8041 tgggcttttt atgtttttta caaggaaggg tttaattcac atatcacctt tattttgttg
     8101 ggcgcaacca ttgccgtatt tattgatttt ctgatcaata ttctggttgt gaaagtgagt
     8161 atgcatacaa caggcgccgg cggtctagtg gcatttatgc tggtgttatt tccttatgct
     8221 caaataaatg tattgccggt attactggct tccattgtaa ttgcgggatg tgttggtgcg
     8281 gccaggttgg cactgaaagc acacactgag cgtgaagttt atcttggtta tcttacagga
     8341 ttcttcagtt ttatggtagc ttcaaatttt attcacctgt aaacggattt atcgctgcat
     8401 aatctttgcg gtgcatccat cctatcacac catccggcat ttctattttc gaccagctac
     8461 tgtcggcctc aattaatttt aatttaaaac cttcgtgaag taaagccagg tttgtgctag
     8521 tttcggaagg ttcgcttttt attacggcac ttggcgacat tacaattgcg aatgtttggc
     8581 cggcgtcata attttttctt ccaaaatgtc ctatagtgca taaaatgcta atcgaaaatg
     8641 caatgatagc tgtaaagaac ccgatgcgct gatatgcgcg gaattgaggt aacagatatg
     8701 cagcccatcc cattaatgct aaccacatcg caactatggc tagtattccc catgcttgtg
     8761 cgcccattat tcgggaaaca gcgtgccacc aaataataaa tatcgatgta gtgcgtggct
     8821 ccatcctgtc gcgaatgcga gcattggcaa gcgaaagatt ataagtagcg tcatcatttc
     8881 caggatcgag aaaaagtgct cgctcataat ttaatacagc ctcgccaagt tgaccggttt
     8941 tataatagca gttgccaata ttgtaatata atgttgcact cggaccttcc tgttctaata
     9001 atcggttata aatgtcgata ctggttgcat attgaccttt ttcatacatc acatttgcgt
     9061 aaacaaaagt ggtggtgtca atattattta ttccttcagt agaagtgctg tcagggtttg
     9121 cctttaaaca caaacttgaa gcgagtagta tgacgatagc aaatactttg ttcatcgcaa
     9181 ttcgttttca agtgtactga tcaatgacga agcactattg aatgttagct ccaaatcgct
     9241 atgttgaatg tttggcgcat acagtgaaaa ttcacaagta tcaagaagct gaataaattg
     9301 atctattgtt gaagcggaaa catgtttgtt caataagacc tgttcaatat tttcttttga
     9361 aagggtattt tgtggaatat gcaatttatc gctcaaataa ccccacaatg cgcgaacagt
     9421 ttcgtcataa aatccgcgac tattattctg ttgcaaaaat ttgccggctg ctgcaaggcg
     9481 ttttttagct gtggcattcg cttttactgc tcgcatggcg ccaacatcgc tcattttcgc
     9541 tttttgccgc cttgtgaaaa caaataatcc cgctcctccg atggcaggtg tgagaatcaa
     9601 tgccagcaga aatccgcttt tcatatcctt ggttccggag gaaaattttg gactatcctt
     9661 atcgatgtac cgaatgtctt tccccaacaa ttctacgtct tccttattcg ctgcgtaatt
     9721 atttgctgat ggattataac ccgggccggc agttaccttt acaggatatg catctgtttg
     9781 caaagtctga tattttttta ctgaaggatc gaagtacgac catgtaaacg ccggaatggt
     9841 aaaatctcct ggagaacggg gaatcaataa atattcgaat attttggtgc ctgaagattc
     9901 acttactttc gggtcgtatg tttcccatcc tgcaggaagg tctaattctg gagcattgaa
     9961 taattgcaaa ttacctgtcc cgttaattgt aatgcggtag gtgagtggtt catcagtttt
    10021 tgtagaggtg ctattgattt gcgaactcat ggtgaacttg cctacagccc cattaaaatc
    10081 tgcaggtgca tttgccggaa gaggcttgat tttaatacct gttgttttag agcttaatga
    10141 agctgtgaac tctctgctgc cactggaaaa agggtcgctg aagaaatcat tcagcatatc
    10201 ctgaaatgga tcacccgaat ttttcttttg tttttttacc cgaacaacaa cacctgcatc
    10261 taatgaagtc ggatcaatca ctaaattccc cgattgttgt ggagtaagtg ttgttttttt
    10321 aatgataaca gttttatagt tcttaccatt gatgttctct gccttaaatt gttcgtttct
    10381 taaatcggca tcctctacat aaaaaccatc gtaagcaggc gttttggtaa tgcgatatcc
    10441 atttaaccct gctattgtac cgtcatcagg tatgcaaagt ttcagcacca ctgtaaagct
    10501 ttctccttcg taaacatctt gatcgctgat ctctgtttta atgaggagat tttcacgcaa
    10561 atattgctca atgtcgccgg aagctgatcc ggatgatcca ttattgctcc ctgaattatt
    10621 attctgtgca cccgcagttg ccggcggcgc atctgttact tcgattacta tgggttgcga
    10681 tgaataggtt ttatccttca ctttaatata agcagcgccg atttgaaatt ttccggtttt
    10741 cttgggctgc agaatataag agaaactcat cgactgttgc accgtattat taaacatctg
    10801 aacactggtc gattgatttg gccctccaag tacagtaaaa tcggtgaaag aaggcgggcg
    10861 gaaattttca ggattgctgc cattgttcag tgtgataata aactgaaaac gctcattcac
    10921 actcaccttg tttttattta ccgtggcagt gaatgacacc tgtgcatgca aagattcagg
    10981 caatatacat gccatagtga ctaaacacat gaatatcagc acattacaac gtctcattac
    11041 cagtcttttt cagggtcccc tcccgcgggg cgggcttttt gtttgttcac tttatcttgc
    11101 acagctttat cgtcttcatt cagcgatttt aatatcctgt cgagatcttt tttgtcaggc
    11161 gtattaggct caggttgttt atcctgattg ttgttttgcg gattgttatc ctgttgctgc
    11221 tgcttttgct tctgctgttg ttgctgttgt tgtttttgtt gatcttgttt ctgctgttgt
    11281 tgctgttgtt gttttttcag catttgttgt gcatacgaca ggttgtattt tgtatcggca
    11341 tcttcaggct tatcgcgcaa cgatttttta taggcatcga tacttcccga gtaatctttt
    11401 ttttcaagta aggtatttcc aaggttatga tatgcctgtg aacgaatttc agggtcttta
    11461 gttccttcgg cggctttact gaactgttcg gctgctgcat catattgttg ctgcatatac
    11521 aagctgttgc caagattgaa gtttccttct accgattccg gtgaggcttt tacggcttcc
    11581 tgataatttt cacgggcacc atcgtaatct tgcttatcgt atttggcatt gccttccttg
    11641 ataagttcat tggcattttg tgcagtaagc gtccgcacgg caaaaatgaa tgctattgcc
    11701 aacaggtgtt tgatattatt ttttttcatc atcaaacaaa tctatttttt ctacccactt
    11761 cggtttttta tctgtcatga ggaattccaa aattaatagg aacaacccaa ttcccagaaa
    11821 taattggaag tggttgacgt attcagtaaa ttcaaaatcg ccatcaacag tcttatcgag
    11881 ctcatcaatg gttttatata cttcttgtat aactcttttt ccttcggcaa ggttcatgta
    11941 aacgccatct gcatctgtag ccagatcgtt taacatttgt tcattgagtt tggtaagtac
    12001 aatattgcct gagcgatctt tcttaaaccc ttcaatatta ttattcttac gtagcggaat
    12061 cggaccacct gtggatgtgc ctacacccag cgtaatgatt ttaattcctt ccgaagcggc
    12121 tttttttgcc atctcttctg cattgtcgtc atggctctcg ccgtcggtaa tgatgatgat
    12181 ggcgcgaccc tttttatcat ctttctcccc accattttcg aacgagagca tggcggtttc
    12241 aattgcactt ccgatagcag tgccttgcgt aggaacactt gttgtgttga tggtgttcaa
    12301 atacatcatg gcagcacgtg catctctggt taaaggcatt tgcacataac tgttacctgc
    12361 aaacactacc agaccaactt tatttgcggc aagtttttca atgagttccg aagcggtgaa
    12421 ttttgcctgt gtaaggcggt ttggtttcac atcctcagcc agcatgctgt tcgaaacatc
    12481 aaaacaaata attacctcgg cgctttcacc acttaccttt tgttctttag atccctgacg
    12541 aagattggca atgcctaaaa tgagaaaagc aatacccaat aattgaagac tatattttat
    12601 ccatctcctt ttatcggaat cgcggaacat taggtcgcga atcaattcac tatcaccgat
    12661 ctttctgatc cttgccgtac gccacctcaa ataccaccaa tacatcagca gcaatgcagg
    12721 aagcaatatg agtagatata atatgtcggg acgttcgaat gacattatgt tagtgtgcgg
    12781 aatattgttt ttgaaagtat cacctctagt aaaatacaaa ttaacccgcc tactgcaaac
    12841 caataaaaat gttcatcgta acggcgcgag atatccattg aaaatttggt tttttctatt
    12901 ttatcgatct gttgatagat atccgttaat ttttcttttg aatcagcttc gaaataggag
    12961 ccacctgtaa tcgacgccac attttttagc attggcgcat caaaggaaat tcgcggatca
    13021 agatcaaaaa tatgtctacc gtttttatcg aacaccggta tcggactgtt agtatttgtg
    13081 ccgatgccaa tggtatacac gcgcatgttc aattcctttg caccattggc agcatcaagt
    13141 ggcgaaaatt tacctcctac acgcacacca tcagtaagta agatgatcac tttctgagaa
    13201 atattggttg aatccgccag gcgatttact gccatgaata atccgtcgcc aattgccgtg
    13261 ccgtctttca aataataatt gtcggcattg tcaacttgct ccttcaataa attgtgatcg
    13321 agtgttgaag gacattgtgt aaacgcctcg cctgcaaata ccactaaacc gattctgtcg
    13381 tttggcctgc tgtcgataaa ttcttttgct gctgtttttg cagcctccat tctgtttggc
    13441 ttgaaatcaa tggcatacat acttggcgat acatccaatg ccagcataat atcaattccg
    13501 tttgaataca ttctgctgtt actgtaactt gtttgtggac gagccatggc aatcacgata
    13561 agcgcaaagg caagcaaacg caatattaaa agcacagaca taaattttgc ttttccttgc
    13621 ggagcttttg gtatgccgct tgtggtagac atacgcatgg caacaaattt cctgcgctct
    13681 atttttgacc tgaaatatac atataccgga atacctgcca gtaacagaag aaaccaaggg
    13741 tcggcaaatt gtatgtcgct caaaaagctc attcctgttt cggtgtttca gtaattattt
    13801 cgcgtgtttg ttcaatgaag ttgagcacat gatcgaatgc tcgtgtattt tcatcagcaa
    13861 gcggctgtga cttcgcaaat tttgccatat ctgcaagatt aagcagatac ctgatattag
    13921 aggcatgtgc ccttgtgtcg ggatgtcttt ccaattgctc aactatttca tcactggtgc
    13981 tttcatgtgc atgcatgtga tagcgctctt ctaaataatc gcgcaagata tcagtcagtt
    14041 ctgaataata ggttttcaac tgacccttct gccatagttg tttgctttgc aattgttgaa
    14101 ggcgttccgt catacgctca tgcaatgatt ttagtggaac gggtacagca atgggtccga
    14161 tatttttcct gcgcaggaaa aaataccaaa tcaatccacc tattataatt aatcctgtaa
    14221 ttattcctac ccaaagtatc catttatgat tgcgtagcgt aacatctaac accgatttta
    14281 ttggtttgat ggcaagtgta gtgtcaacgg caattgcttc aatatgcaag ggaatagaat
    14341 cagtgagtat ttgatgttga gcggtatcac ttgtagactg gtaattaatt gctacctgcg
    14401 gaaaatatac atcgcctcct tcgtagatac tgtaaatgat atgctttgtc aggatgacac
    14461 ggtcgtcgct gcgctgtgta tctatcggca tgctgctgat gatgtcgtag gtgctgattg
    14521 tggtttcatc aggaaaataa tatttaaagg atgccggccc gcttatggta atatctacag
    14581 aaatataatc gccaatgcta tatgaaggtt tgtcggtatt agcatgagca gatattatct
    14641 gaccctgaag ttgattacag gtaattatta tcaataatat gacaattaat ttcttcatca
    14701 tttatcgcat gctgattctt ttcttaaaaa aggcgtgtag tgcattgata tacgataatt
    14761 cactgtgtat agaaatggta tcagccccac tgcgcaaaaa ggtgtttttg aaatactgca
    14821 gattggtgtt gaagtatttc ttataggaag cgcgaacagt tttatttgaa ctgtctaccc
    14881 attgaatatt gcgtgattcc gggtcaaaca tgcgaatgat gccggcgtcc ggaagatctt
    14941 cttcgcgctt gtcgtgaata tgtacaccta ttacatcatg ttttctcgcc gccaccatca
    15001 atggcttctc ataatgcata tcgaaaaagt cgctgaacat aaatgcgata ctctttttct
    15061 tcaccacatt attgaaatat tccaatgcca gtgcaatatc cgttcctgag ccttcagctt
    15121 ttacttctaa caggtcgctg atgatacgaa gtatatgtga ctttcctttt tttggcggaa
    15181 tgaatttttc tattctgtct gaaaataata tcaccccaac cttatcattg ttattgatgg
    15241 cagaaaagct gagtaccgca gcaatctcgg tgatcagcgc tttttttgtt tgcttctgtg
    15301 tgccgaataa agaagatgca ctgatatcaa tcagcaacat caccgttaat tcgcgttcct
    15361 cttcaaatat tttaatgtaa ggatggttca tgcgagcggt aacattccat tcaatattgc
    15421 gaatgtcgtc gccataagca tattcgcgca cttcactgaa actcatacct ctacccttga
    15481 aggcagaatg atattcgccg ctaaagatat tctgcgagat cccttttgtt ttgatctcga
    15541 tgagtcttac cttttttaat aaagttgctt tatccatccg ctttagatca acgtttcttt
    15601 gtggtgatca ttaaggcacc tccactacat tgagcaattc agatatgatt tgttctgccg
    15661 ttatattttc agcctcggct tcatatgtca atccgatgcg gtgacgcatt acatcgcgac
    15721 aaacagcacg cacatcttca ggaattacat atccgcgtcg cttgataaat gcataagcct
    15781 tgcttgcgag agccagattg attgacgcgc gtggtgaccc accatatgca atcaaatgtt
    15841 tcagcttacc aagtttatat gcttccggat cgcgcgttgc aaatacaata tcgagaatat
    15901 agttttctat tttctcatcc atatatactt cgcgcaccag gtctcttgct ttcagaatgt
    15961 cttcaggttt tacaacttgt ttaatttgtt gtatggcacc gctcacgttc tgacgaatga
    16021 tcattttttc ttcctctttt tcaggatagt gtatcactac tttcagcata aaacggtcta
    16081 cctgtgcttc aggaagcgga taggttcctt cctgttctat cggattctgt gtagccagta
    16141 ccaaaaatgg ttcttccagt ttgtacgtat gctcgccaat ggtaacctga cgttcctgca
    16201 tcgcctcgag taatgcactc tgcactt
//
LOCUS       DAOQHY010000068        16086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1707_length_16086_cov_5.803069, whole genome shotgun sequence.
ACCESSION   DAOQHY010000068 DAOQHY010000000
VERSION     DAOQHY010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 16086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16086
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1707_length_16086_cov_5.803069"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..1203)
                     /locus_tag="PLL28_07255"
     CDS             complement(169..1203)
                     /locus_tag="PLL28_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNF69157.1"
                     /translation="MSSPVIHFAIIGHGHIGKRHAAVIRETPGAIVTAICDVDAQKLA
                     GIDEGVAVFTSITEMLNSNLPIDVVNICSPNGLHAEHALSCLQHNKHVVIEKPMALQV
                     ADCDAVIKLAKDKNKEVFCVMQNRFSPPSVWIKSMLDTHALGKICMVQVNCYWNRDER
                     YYTGNSWHGTRDFDGGTLFTQFSHFIDTMLWLFGDISDIHGIFDDYKHKHLTDFEDSG
                     MVHFRFINGAIGSLQYSTAVYDKNFESSIRIIGEKGTVCLGGQYMNEVTYCHVQDYVM
                     PELAPTAAPNNYGPYIGSAANHHFIIQNVLDVLHREAPIATTAEEGRMVVDVISRIYA
                     LRAQHITQKN"
     gene            complement(1211..1795)
                     /locus_tag="PLL28_07260"
     CDS             complement(1211..1795)
                     /locus_tag="PLL28_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HNF69158.1"
                     /translation="MSKQSHFIHSSAVVDDGAIIGKGTKIWHFTHIMPEAIVGEDCVI
                     GQNVFIASRVEIGNRVKIQNNVSLYEGVMCEDDVFLGPSMVFTNVINPRSAVERKNEF
                     QLTRVLKGASIGANATIICGITIGKYAFIGAGAVVTKSVPDYALVTGNPARQKGWMSA
                     HGGKLIFDSKGHAVCDISGINYKLSGGRVQQLNN"
     gene            1847..3325
                     /locus_tag="PLL28_07265"
     CDS             1847..3325
                     /locus_tag="PLL28_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69159.1"
                     /translation="MRINSQFALMIMGLGALLTGSCSRDFLGEEALNLAPETYLVTDT
                     IIRFGDDRLESEVTLQWWGDDEDGFVTGYEFTFDEVITDATIWQYTESQDSTFILAPP
                     PGEDSADFVFHIRAIDNHNLADPTPAALTVPVKNSPPAIIIIPGLNTPKKSFPALRYY
                     WESSDPDGAQNLSHVELFFNDTTGTPYILPSTVTSAIFQAQDPTALTLNCEVFANGGT
                     IAEDILMNGLMANAWNKLYVRAVDQSNAKSAFAIADSVFVKQVHSTVLLVNGYSTATN
                     EAFYMSHLSANGIAVVDTMQIFETVGGVYTQQSADNLTQGRIFAMFDAIIWFSNNADN
                     SFSLAQKTTESFFNNGGKMLMSVYISSSFDPLSNFLDFTPIASLVNPVDTTLILDLGA
                     SLSAEVADWPDLQGTSIVGVVKPINLQIGATPLYTATLTAKDNVALTFTPWTGVSTVM
                     ASKTDGAGQTNFVISTLELNKLDGLLNMDSFFEKVMIDEFGF"
     gene            3309..3920
                     /locus_tag="PLL28_07270"
     CDS             3309..3920
                     /locus_tag="PLL28_07270"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNF69160.1"
                     /translation="MNSDFEMHVAGGDHRIAIFASGAGTNAENCIRYFRTHASIYVAL
                     IVTNKPTAGVIEVAERYGIPYMIQETWDDEGTELLLSRLAQHRISFILLAGFLSLVPN
                     ELIRKFTGRIINIHPALLPAFGGKGMYGKRVHAAVYAAGVGESGITIHEVDEEYDHGK
                     ILFQAKVAIDGQDTPASIEEKVRALEYAHLPAVVEKLLLTGRV"
     gene            3999..5540
                     /gene="purH"
                     /locus_tag="PLL28_07275"
     CDS             3999..5540
                     /gene="purH"
                     /locus_tag="PLL28_07275"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNF69161.1"
                     /translation="MQIEHHPVKIKSALISVYYKDKLEPIIHALHRLGVVLYSTGGTR
                     SFIEDLGVPVQAVEDLTLYPSILGGRVKTLHPKVFGGILARRASNEDQQQLTQYHIPE
                     IDLVIVDLYPFEETIRHTTDHSEIIEKIDIGGVSLIRAAAKNYHDVLIIASKDDYSAL
                     QDILDAGGETKLGQRMYFAKKAFNITSSYDSAIFGWMDHGEHLDLRYAPKIGMRYGEN
                     PHQQAAFYGHLSEVVEQIQGKEISYNNLVDIESCIYLMEEFEASKEAGKATFAIIKHT
                     NACGLATADSVESAWDKALAADPVSAFGGVLICNQAIDKAAAGKINELFFEVIIAPDF
                     DAEALALLQSKKNRIILRQRMFKFPLKQFKTVLNGVLMQDRNVKATRADELMKVTDIH
                     PTEAQIQDLLFAEVVVKHLKSNGIALVRNGQLIGCGTGQTSRVDALKQALAKAALFGF
                     DVRGSVMASEAFFPFPDCVQIAHEAGISAVIQPGGSVRDKDSIDYCNTHGLPMVLSGT
                     RHFKH"
     gene            5717..6742
                     /locus_tag="PLL28_07280"
     CDS             5717..6742
                     /locus_tag="PLL28_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNF69162.1"
                     /translation="MGIFNFLTQEIAIDLGTANTLIIAHDKVMVDEPSIVAIDRKTEK
                     VIAVGKKAMQMHEKTHENIKTIRPLRDGVIADFQAAESMIRGMISMIFPKNRLFKPSL
                     VMVICIPSGITEVEKRAVKDSAEQAGAREAYLIHEPMAAALGIGLDVQEPVGNMIIDI
                     GGGTTEIAVVALSGIVCDQSIRVAGDEFTADIVDYMRRQHNLLIGERTAESIKINVGA
                     ALTELDDAPEDYQVSGRDLMTGIPKQLVVTYSEIAHSLDKSISKIEEAILKALETTPP
                     ELASDIQRNGIYLTGGGALLRGLDKRISQKTKLVVHVADDPLKAVVRGTGIALKNREQ
                     YAALLMS"
     gene            6759..7592
                     /gene="mreC"
                     /locus_tag="PLL28_07285"
     CDS             6759..7592
                     /gene="mreC"
                     /locus_tag="PLL28_07285"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNF69163.1"
                     /translation="MRSFFLFIIRHYAIFMFLFLELLSFYFIVTHNSYQQTYFINTAN
                     TLSGNVLNSYGNFQEYFSLREVNDSLMHENAMLREQLLLSQIADTVNHTVSDSTGKPL
                     YSYIPAEVIGNTFTEANNYITLNKGSSDGIQVNMGVITSGGIVGRVVKVSPNFSVVMS
                     VLHTQFVSRVAVKRSNAQGRLTWDGKRPTHIDVIQVSEPGTIKKGDSIITTKVSPMFP
                     PDIMVGTLESIGKDPGSNYYTLDVKLSTNFSSLKYVYVVNEIMSKERNDLENQIQDAG
                     N"
     gene            7579..8103
                     /gene="mreD"
                     /locus_tag="PLL28_07290"
     CDS             7579..8103
                     /gene="mreD"
                     /locus_tag="PLL28_07290"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNF69164.1"
                     /translation="MQVINWILRVVGLVFLIMFQVLVLNRLNVSEYVHPYVYPMFIIL
                     LPFDTPRWMLLVVAYLIGFTIDMFNNTPGMHAAACVFIAYFRPQLIKFYTPITGYESV
                     TSPSISELGVIWFTMFTATTILLHHAVYFLIQLFSINDLGFLMIRIGLSTVVSTLLIV
                     ILAFLFAKRKVRAK"
     gene            8228..10135
                     /gene="mrdA"
                     /locus_tag="PLL28_07295"
     CDS             8228..10135
                     /gene="mrdA"
                     /locus_tag="PLL28_07295"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNF69165.1"
                     /translation="MAVIENLKPRGYVIQAVIAAIFVVFIVRLFYLQVIDDTYKQIAD
                     DRGIRKEQVDPDRGQIFDRNGKLIVYNEPSYKLMVVPSQVKDIDTIRFCEIIGVDKEY
                     FEKKMKEAKNFSRVRESVFLSQISAEDFGRIEEYLYEFKGFYTAITPVRRYTYPAAGH
                     LLGYIAEVDSNDIKKSDGYYRNADMIGKTGVEKSYEKLLRGKKGYHNIVIDRFGRAVG
                     PLNNGSEDVEPVAGKNLTLTIDIELQYYAEQLMVGKRGALVAIEPSTGEILALVSSPT
                     YDPNMLSGSARNKNFAKLALNKENPLNNRALSGYYPPGSTFKPVMAALGMQQGSLTST
                     TGYSCPHGYVMSGHTVDCHNHPYPANVELGIGHSCNAYFCYAFRYFIEDHSNPAEGMR
                     DFKAKLALWGIGTRTGIDLPNERNGNVPSPEDYDKIYGKGRWKASNCVTLGIGQDRLI
                     LTPLQSANSMATIANGGYFYSPHVLKFADGEDSLMNAFTIRKETGISDSIFKYVRHGM
                     AGAVNFGTAKVAHIDSIQVCGKTGTAENPHGKSHSWFSCFAPEVNPKIAVAIIVENGG
                     WGATYAAPIASLVVEKYMNDSISAKRKPLEERMMKSVLILDREVVPPIQNNSNQDTTK
                     QVQAALLPKEQ"
     gene            10132..11559
                     /gene="rodA"
                     /locus_tag="PLL28_07300"
     CDS             10132..11559
                     /gene="rodA"
                     /locus_tag="PLL28_07300"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNF69166.1"
                     /translation="MREKKSIMENVDQPLLWLYLSLMVIGLLNVFAVSFSDDVKFFDL
                     SLPHGKQFLWMGISLVVGFSILTFESSFFTKFAMVLYGVTIAVLIVTFVVAVDINGAR
                     SWLQVGSFQFQPAEFGKTTTALMLAKFLSIINGQPRSFKNKLLSYLIIGVPMGIIVLQ
                     SDVGSALVYASLVLVVYREGFPVYEVLFVLAIGLCFVFALIFGVDPVAVVLTLIIWLY
                     IVFSSFKLVRKEKIKGIVFLVIFSAGVFFFLIGGEVLGMDTHIAAAVMLIAMIAALIN
                     VRKVRFFQMNIPVVLFAILMVFVMFGVETITTDILEDYQVQRIETLLGMSDDADAEYN
                     VTQSKMTIGSGGVFGKGYLQGTLTQSEQVPEQSTDFIFCTIGEEWGFFGTFIFLVIYL
                     LFILRVIFIAERQRSPFSRVYAYCVASFFFLQIMINVGMTIGLIPVIGIPLPFISYGG
                     SSVLAFSIMIFIMLRLDADRLFILR"
     gene            11646..13127
                     /gene="hutH"
                     /locus_tag="PLL28_07305"
     CDS             11646..13127
                     /gene="hutH"
                     /locus_tag="PLL28_07305"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HNF69167.1"
                     /translation="METFHIGIQGLTLQQISDIIKNKIRIALSDEAREKIQKCRTYLD
                     NKIASQDKAIYGVNTGFGSLCKVKISDSDLEQLQYNLIRSHACGMGAVIPDEICRILL
                     LLKIQNLAFGHSGVAVETVWRLIDFYNMDLLPVIYEQGSLGASGDLCPLAHMSLPLIG
                     EGELKVNGKIRPTAEVLKEAKLSPLKLQSKEGLALLNGTQFMAAHAVYTLIKADNIIR
                     QSTYIAGVSLEAYDCKSEPFTDQIHQIRGQHGQIAIAKAFMHIMQGSELFFAEKQQVQ
                     DPYSFRCIPQVHGACYDTIQYCKTIVEREINGVTDNPNVFVEEDMILSGGNFHGEPLA
                     LTLDFLAMALSEMGNISERRVYRLLSGARGLPEFLTPDPGLHSGLMIPQYAAASIVSQ
                     NKQLCTPASVDSISSCNEQEDHVSMGANAATKCLRVAINVERILAIELLTAAQAFEFR
                     RPAKTSPALENYIASYRQHVTFNTEDRFLHKDMLTTEKYLQMV"
     gene            13280..13660
                     /locus_tag="PLL28_07310"
     CDS             13280..13660
                     /locus_tag="PLL28_07310"
                     /inference="COORDINATES: protein motif:HMM:NF024759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNF69168.1"
                     /translation="MHINQLSGVIVDILIHMHKELGPGLFESVYEEVLASELDKREIG
                     YRRQQVIPIVYDDVVLVENAFKADIIVDNRIIIELKSIDQLSPVHYKQLLTYLRLTGI
                     KLGLLVNFNEVLIKDGIHRVVNNL"
     gene            complement(13692..15461)
                     /gene="aspS"
                     /locus_tag="PLL28_07315"
     CDS             complement(13692..15461)
                     /gene="aspS"
                     /locus_tag="PLL28_07315"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNF69169.1"
                     /translation="MHSNTFYRTHTCGELRMQSVGTTVTLSGWMHRSRDLGGMTFIDL
                     RDRHGITQLVFNMESNAALCEMARKLGREFVICASGVVTERSSKNAQLPTGDIEIIVD
                     KLEILNAAELPPFTMDDETDGGEELRMRYRYLDLRRPQMQQRLMLRANMLKAVREYLD
                     AEGFVEVETPNLIKSTPEGARDFLVPSRMQPGLFYALPQSPQILKQLLMVSGMDRYYQ
                     IAKCFRDEDLRGDRQPEFTQIDCEMSFVRQEDVWNMFEGMTKHVFKKVKNIDLPDFIR
                     LPYRDAMKYYGSDKPDLRFDCKIQDLNAVVGGSEFGVFNNVLASNGLVAGIVAKGCSE
                     YSRKQIDELTEFVKAPQRGVGGLIYIKFSQDGSVKSSMDKFFTPEQLIAIGESVGAGK
                     GDLLLIVADKEKKTRKVLGDLRLEMAKRENWIDKNAWSVFWVVDMPLFEPDEETGEPI
                     FAHHPFCSPHPDDFQYMDSDPLRVRAQSYDMVMNGNEILSGSIRIHNKELQEKIFKIL
                     GFSEEEKERRFGFMVNAFKYGAPPHGGCAFGFDRWVMLMAGGDTIRDVIPFPKNNNGR
                     DMMMDAPGEVDNDQLRIVGVKLL"
     gene            15569..>16086
                     /locus_tag="PLL28_07320"
     CDS             15569..>16086
                     /locus_tag="PLL28_07320"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF69170.1"
                     /translation="MHCVSCGHTHFSATPYYYQWNSKEFVLTTCKNCGLITMDPKPTD
                     EELGLLYADEYFETGQHGLNRIGMTYEQGQDKNGIDHRINAVKQNMLAFKPDMQSVFE
                     IGFAMGHMLAAARDLGLEIGGIEFSPMAVSRAKEKFNIDALCGNFENIDLSAHLHKWD
                     CVYGGDVFEHFI"
BASE COUNT         4522 a         3258 c         3615 g         4691 t
ORIGIN      
        1 tatatccgct gaacatggga atggaaacga tcttttttct gtcactccat tggcgagcag
       61 attttatcaa aggcaagtag caagtaatgc catttttttc aaggcgttct gcaacttttt
      121 tttcactgcg tgagcgcaca tatgccacat accaatgcgc cgccatattt aatttttttg
      181 agtgatatgt tgcgcgcgca gtgcataaat gcgtgaaatc acatcaacaa ccatacgtcc
      241 ttcttcagct gttgtggcta ttggtgcttc gcgatgtaaa acatctaata cgttctgaat
      301 aataaaatgg tgattggcag cagaaccaat gtatgggcca taattatttg gcgcagcggt
      361 aggcgctaat tcaggcatta cataatcttg cacatgacaa taggttactt cattcatgta
      421 ttgcccacct aagcatactg tccctttttc acctataata cgaatgctgc tttcaaaatt
      481 tttatcatac acggcagtac tgtattgcaa gcttcctatg gcgccattta taaagcgaaa
      541 atgcaccatc ccgctgtctt cgaaatcggt aaggtgttta tgcttatagt cgtcgaaaat
      601 gccgtgaata tctgagatat cgccaaacaa ccaaagcatg gtatcaatga agtgtgagaa
      661 ttgagtaaac aatgttccgc cgtcgaaatc tcgagtgcca tgccatgaat tgccggtata
      721 atatctttca tcgcgattcc agtagcaatt cacctgcacc atgcaaattt ttccaagtgc
      781 atgagtgtct aacattgatt ttatccagac cgatggcgga gaaaatctgt tctgcattac
      841 acagaatact tccttgtttt tatcttttgc cagtttaata acagcatcac aatctgcaac
      901 ctgcaaggcc atgggttttt ctatcaccac atgtttattg tgttgaagac atgacagtgc
      961 atgttcggca tgtaatccat ttggcgaaca aatatttacc acatcaatag gtaaattgct
     1021 attcagcatc tccgtgatgg aagtaaatac tgcaacacct tcatcaattc ctgctaactt
     1081 ttgagcatca acatcgcaaa ttgcagttac aatcgcaccc ggtgtttcac gaataactgc
     1141 ggcatgtctt tttccgatat gaccgtgacc aatgattgcg aaatgtatta caggtgacga
     1201 cacgatgatc ttaattattt aattgttgta ctctgccgcc ggaaagttta taattaatcc
     1261 ctgaaatatc gcaaacggca tgacctttag aatcgaagat taatttaccg ccatgggcac
     1321 tcatccatcc tttttgacga gcaggatttc ctgttaccag tgcataatcg ggaacagatt
     1381 ttgtaactac agcacctgca ccgatgaaag catatttccc aatagtgata ccgcaaataa
     1441 ttgttgcatt tgctccaatg ctggcacctt ttaatacacg tgtaagttga aattcatttt
     1501 tacgttcaac agcacttcgc ggattgatga cattggtaaa taccatgctt ggaccaagaa
     1561 agacatcatc ctcacacatc acaccttcat aaagcgatac attgttttga atttttactc
     1621 ggtttccgat ctccaccctg ctggcgatga atacattttg accaatcacg cagtcctctc
     1681 ccacaatagc ctcaggcata atatgcgtaa aatgccaaat ttttgtgcct ttacctataa
     1741 tggcaccatc atcaacaacg gcggacgaat gtatgaaatg cgattgcttc gacatgatta
     1801 tgggcaaaga taagtgtttg acgagaagca ttatcttcgc gctgtgatgc gcataaacag
     1861 tcaatttgct ttgatgatca tgggtttggg agccttgctg acagggtcct gctctcgcga
     1921 tttccttggc gaggaggctc tgaatttggc acctgagact tacctcgtta cagataccat
     1981 tatccgtttc ggcgacgaca ggctggaaag tgaggttacc cttcagtggt ggggcgatga
     2041 cgaagatggc tttgtaacag gctatgagtt tacattcgat gaggtgatta cagacgctac
     2101 catctggcag tatacagagt cgcaggacag tactttcatt ctggcccctc ctccgggaga
     2161 ggattctgcc gatttcgtat tccatatccg ggcgatagac aatcataatc tagctgaccc
     2221 gacacctgca gctttaacag tacctgttaa aaacagccct ccggcgataa ttatcattcc
     2281 cggcctcaat acccctaaaa aatcattccc tgctctgcgc tattactggg aatcaagtga
     2341 ccccgatggg gctcagaacc tgtcgcatgt agaattattt ttcaatgaca ccaccggcac
     2401 gccatatata ctgccgtcga ccgtaacgag tgccattttt caggcacagg atcctacagc
     2461 attgacactt aattgtgagg tgtttgcaaa cggcggcacg attgcggagg acattttgat
     2521 gaatggattg atggcaaatg cctggaataa attatatgtg cgtgcagtag atcaaagcaa
     2581 tgcaaaaagt gctttcgcca ttgcagattc tgtattcgtg aaacaggtgc acagtacggt
     2641 attgctggtg aatggataca gcactgctac aaatgaagcg ttttatatgt cgcatctcag
     2701 tgccaacgga attgcagttg tcgacacgat gcagattttt gaaactgtgg gaggtgtgta
     2761 tacacagcaa tcggcagaca acctcactca aggaagaata tttgccatgt ttgatgctat
     2821 tatctggttt agcaataatg ccgataattc attttcactc gcgcaaaaaa caactgaatc
     2881 tttttttaat aatggcggta aaatgctgat gagtgtttat atctcttcgt catttgatcc
     2941 attgagtaat ttcctcgatt tcacaccaat tgcttcattg gttaatccgg tagataccac
     3001 attaattctc gatctgggcg catcgctttc ggctgaagtt gccgactggc ctgatcttca
     3061 ggggacttct attgtggggg tggtgaagcc aataaacttg caaataggtg ctacaccttt
     3121 atatactgct acacttactg caaaagataa tgttgcgttg acctttacgc catggacagg
     3181 tgtgtcgaca gtaatggcta gtaaaactga tggtgcaggg caaacgaatt ttgtgatttc
     3241 tacactcgaa ttaaataaac tggatgggct gttgaacatg gacagctttt ttgaaaaagt
     3301 gatgatagat gaattcggat tttgagatgc atgttgcagg tggtgatcat cgcatagcca
     3361 tttttgcatc aggtgccgga actaatgcgg aaaattgtat tcgttatttc agaacacatg
     3421 cttctatata tgtagcatta attgttacca ataaacctac agccggagtt atcgaagtgg
     3481 cagagcgata tggtatacca tatatgattc aggaaacctg ggatgatgaa ggtaccgaat
     3541 tattattatc gcgattggca caacatcgca tttcatttat tttattggcc ggttttttga
     3601 gtttagtgcc gaatgaatta atcaggaaat ttaccggtcg tattatcaat atccatcctg
     3661 cattgttgcc cgcatttggt ggtaagggca tgtatggaaa acgcgtgcat gcagcagtgt
     3721 atgcggcagg agttggtgaa agtggcatca ccatacacga agttgatgaa gaatatgacc
     3781 atggtaaaat tttatttcag gcgaaggtgg caattgatgg ccaggatact ccggcctcaa
     3841 tagaagaaaa agttcgagca cttgaatacg ctcatctccc cgctgtggtg gaaaaattgt
     3901 tgctaacagg cagagtttga cctcataccg taattgacac gtatgaatac actgcttttc
     3961 tgctgtagag gcggtaattt tgcactttct ttcacattat gcaaatcgaa caccatcctg
     4021 ttaagatcaa gtcggccttg atctctgttt attacaaaga caagctagag ccaattattc
     4081 atgcactgca ccgacttggg gtagtattat attcgaccgg aggaactagg agttttattg
     4141 aagatctcgg agttcctgtg caggctgtgg aagacctgac gctgtatcca agcattttgg
     4201 gtggtcgagt gaaaactttg catccaaagg tttttggcgg aatcttagcc agacgcgcgt
     4261 cgaatgaaga tcagcagcaa cttacccaat atcatattcc ggagatcgac cttgtgattg
     4321 ttgatttgta tccgtttgaa gaaacaattc gacataccac cgaccatagt gagatcattg
     4381 aaaagattga catcggtggc gtatcactga tacgagcagc ggcaaagaat tatcatgacg
     4441 tgctcattat tgcttcgaaa gatgactata gtgcgttgca ggatatactt gatgccggag
     4501 gtgaaacgaa attgggacag cgtatgtatt tcgccaagaa ggcatttaat atcacgtcgt
     4561 cttatgacag tgccattttc gggtggatgg accatgggga gcatcttgac ctgcgttatg
     4621 cgcctaaaat cggcatgcgt tatggtgaaa atccgcatca gcaagcggcc ttttacggtc
     4681 atttgagtga ggttgttgag caaattcaag gaaaggaaat atcctataac aacctggtag
     4741 atattgaaag ctgtatttac ctgatggagg agtttgaagc atcaaaggag gcagggaaag
     4801 caacttttgc cattatcaag cataccaatg catgcggact ggcaactgca gacagcgtag
     4861 aaagtgcctg ggacaaggct ttggcggcag atccggtgtc ggcattcgga ggggttttga
     4921 tctgtaacca agctatcgat aaagccgctg caggcaagat caatgagtta tttttcgagg
     4981 tcatcatagc gccagatttt gatgctgaag cattggcact tttacaatcg aaaaagaaca
     5041 ggatcatatt gcgtcagcgc atgttcaaat tccctctgaa acagttcaaa actgtattga
     5101 acggcgtatt gatgcaagat cgcaatgtga aggcaacaag agctgatgaa ttgatgaagg
     5161 ttaccgatat acatccaacc gaagcacaaa tacaagatct gttgtttgca gaggtagttg
     5221 tgaagcattt aaaatcgaat ggcatagcct tagttcgcaa tggtcaatta ataggatgcg
     5281 gcacaggaca aacaagtagg gtagatgcat taaaacaagc tttggctaaa gcagcattat
     5341 ttggttttga tgtgcgtggt tcagtgatgg caagtgaggc cttttttcca ttccctgatt
     5401 gcgtccagat tgcccatgag gcgggcattt cagctgttat tcaaccgggt ggaagtgtgc
     5461 gtgataaaga cagtatcgac tactgcaata cacatggttt acctatggtg ttgagtggca
     5521 cacgacactt caagcattga tattttttat gcttcatttt aatgcttact tttgattgat
     5581 aagcagagat aatttttgcg aataattttc gttcgtaaaa aaagttagtt ttattttgat
     5641 aatttatgtg aaattccttc gcggatacct aacataagtt aattttgcac acttatatct
     5701 ttacgagcag agcatcatgg gcatattcaa ctttctcact caggaaatag ctatcgacct
     5761 gggcaccgcc aataccctca tcattgcaca cgataaggtg atggtggatg aaccatctat
     5821 cgtggctatc gaccgcaaaa ctgaaaaagt gattgctgtt ggaaagaaag ccatgcagat
     5881 gcatgaaaag acccatgaga acattaaaac cattcgaccc cttcgcgatg gtgtgattgc
     5941 cgactttcag gccgctgaat ctatgatcag aggcatgata agcatgattt ttcccaaaaa
     6001 caggctgttt aagccctctt tggtgatggt tatctgtatt ccatcaggta tcaccgaagt
     6061 tgaaaaacgt gccgtaaaag atagtgccga acaagccgga gcccgcgagg catacctgat
     6121 tcacgaacct atggcagcag ccctgggtat cggtttggat gtgcaggagc ccgttggaaa
     6181 tatgatcatc gatatcggtg gtggtactac cgaaattgct gtagtcgcct tatcgggtat
     6241 cgtttgcgac cagtcaatcc gcgttgccgg cgatgagttt acggcagata ttgtggatta
     6301 catgcgccgt cagcacaacc tcctcatcgg agagcgcacc gctgaatcta tcaaaatcaa
     6361 tgtcggcgca gcacttaccg aactcgacga tgctcctgaa gattatcagg taagtggtcg
     6421 cgacttaatg accggtatcc ctaaacaatt ggtagtaacc tattctgaaa tagcccattc
     6481 gctcgataaa tctatcagca agatcgaaga ggccatcctc aaggccctgg agaccacacc
     6541 ccctgaatta gccagtgata ttcaacgcaa cggtatctat cttaccggcg gtggagcatt
     6601 attgcgcggc ctcgacaaac gtatcagcca gaagacaaag ctcgttgtgc atgtagccga
     6661 tgatccgctg aaggctgtag tgcgcggtac agggatagcc ctgaagaacc gggagcagta
     6721 tgcggcttta ctgatgagct aaaccgccac cctgcataat gcgtagcttc ttcttattta
     6781 tcatccgtca ttatgccatc ttcatgttcc tgtttttgga attgttgtcg ttctatttta
     6841 tagtgacaca taattcctat caacaaacat attttattaa taccgccaat accttgtcgg
     6901 gaaatgtgct gaacagctac ggcaattttc aagaatattt ttcactgcgc gaagtaaatg
     6961 atagtctgat gcatgaaaat gccatgctca gagaacaatt attattgagt cagattgctg
     7021 ataccgttaa ccataccgta agcgacagca ccggaaaacc gctctacagc tacattccgg
     7081 cagaagtgat agggaataca tttactgaag caaataatta tatcaccctc aacaaaggtt
     7141 cctccgacgg gattcaagtg aacatgggtg tgatcacctc cggcggaata gttggcagag
     7201 tggttaaagt atctcccaat ttcagcgtgg tgatgagtgt gctgcatacg cagtttgtaa
     7261 gtcgcgtagc cgtaaaaaga agcaatgctc aaggccgact aacatgggat ggcaaacgcc
     7321 cgacacatat tgatgtaatt caagtatcag agccgggaac aattaaaaag ggcgacagca
     7381 ttatcacaac caaagtatcg ccaatgtttc ctccggatat tatggttgga acattggaga
     7441 gcatagggaa ggatcccgga agcaattatt atacacttga tgtaaaactg agcaccaatt
     7501 tcagctcatt aaaatatgta tatgtggtga acgagatcat gtcgaaggaa cggaatgatc
     7561 ttgaaaacca aattcaggat gcaggtaatt aactggatat tgcgtgttgt tggactggtc
     7621 tttctgatca tgtttcaggt attggtgctt aacaggctca atgtgagtga atatgtgcat
     7681 ccatacgtat atccaatgtt tatcattctg cttccattcg acacaccaag gtggatgttg
     7741 ctggtggtag cctacttgat tggttttacc atcgacatgt tcaacaatac ccccggcatg
     7801 catgcagcag cctgtgtttt cattgcctat ttcagacctc aattgatcaa gttttatacc
     7861 ccgattaccg gttatgaaag tgtaacatcg ccatccatat cagagctcgg tgtgatatgg
     7921 tttaccatgt ttacggctac aaccatttta ttgcatcatg ctgtttattt tctgattcaa
     7981 ttattctcca tcaacgacct tgggttttta atgataagga taggtttaag tacggtagtt
     8041 tcaaccttgt tgatcgttat tctggcattc ctctttgcta agcgtaaagt acgtgcaaaa
     8101 taaggatgta aatttttgct ttgctctcga aaacatttct gaattttgta aaaggaattg
     8161 ttgttacagg cgttattgaa ggaacaacat cattatttcc ttccaacacg atttccaaca
     8221 ccaatcaatg gccgtaatag aaaatctcaa acctcgcgga tatgtcatac aagcggtgat
     8281 agcggcaatt tttgtcgtgt tcattgttcg tcttttctat ttgcaagtga tcgatgatac
     8341 ctataagcag atagccgacg accgcggtat tcgcaaagag caggtagatc ccgacagagg
     8401 tcagattttc gatcgcaatg gtaagttgat cgtttacaat gaaccttcgt acaagttgat
     8461 ggtggtgcca tcgcaagtga aggatattga taccattcga ttttgtgaga tcattggtgt
     8521 cgataaggag tatttcgaga aaaagatgaa agaggcgaaa aacttttctc gtgtacgtga
     8581 aagtgtattc ttatctcaga tttcagcaga ggattttgga agaattgaag agtatctgta
     8641 tgaattcaaa ggcttttata ccgccatcac acctgttcgc cggtatacat atcctgcagc
     8701 cggtcacttg ttgggatata ttgccgaagt agactccaac gacatcaaaa agtctgatgg
     8761 ttactatcgc aatgccgata tgataggaaa aaccggcgtt gaaaagagtt atgagaagct
     8821 gttaaggggg aagaagggat atcataacat tgttatcgat cgcttcggtc gcgctgtggg
     8881 ccctttgaat aatgggagtg aagatgtgga accggtggca ggaaagaatc tcacgctcac
     8941 tatagacata gaattacaat attatgccga gcagctgatg gtaggaaaga gaggagcatt
     9001 ggtggccata gaaccttcaa caggtgaaat actggccctg gtaagctcac ctacttatga
     9061 tccgaatatg ctgtcgggaa gtgcccgcaa taaaaatttc gccaaactgg cactcaacaa
     9121 ggaaaatccc ttgaacaaca gggcattaag cggttattat cctcctggtt caacattcaa
     9181 accggtgatg gcagctcttg gcatgcaaca aggttcactc acgtcaacta cgggatatag
     9241 ctgtccgcat ggttatgtaa tgagtgggca tactgtcgac tgccacaatc acccttatcc
     9301 tgccaatgtg gaactgggaa tcggccattc atgtaatgcc tatttctgtt atgccttccg
     9361 ctattttatt gaagatcatt caaacccggc agagggtatg cgcgatttta aagcaaaact
     9421 cgccttatgg ggtattggta cccgaacagg tattgacctg ccaaatgaaa gaaatggaaa
     9481 cgtgccaagt cctgaagact acgataaaat atacggtaaa ggaagatgga aggcttcgaa
     9541 ttgtgtaaca ctcggtatag gacaagacag acttatttta acgccgcttc aatcagctaa
     9601 ttcaatggcg acgattgcca atggtggtta tttttattca ccacatgtat taaaatttgc
     9661 tgatggggaa gattcgttaa tgaatgcttt taccatacgt aaagagacag gtatttcgga
     9721 ttccatattc aaatatgtgc gtcatggtat ggcaggagct gtaaattttg gtacagcaaa
     9781 agttgcacat atcgattcca ttcaggtttg cggtaaaaca ggtacagcag aaaatccgca
     9841 tggaaaaagt cacagctggt tcagttgttt tgcaccggaa gtaaatccga agattgccgt
     9901 tgccattatt gttgaaaatg gcggatgggg agcaacctat gctgcgccaa ttgcaagttt
     9961 ggttgtagag aaatatatga acgacagcat cagtgcaaag cgaaaaccac tggaagagcg
    10021 tatgatgaaa tcagttttga tactggatag agaagttgtt ccaccaattc aaaataatag
    10081 caatcaggac actacaaaac aggttcaggc tgcactttta cctaaagagc aatgagagaa
    10141 aaaaaatcca tcatggaaaa tgtagatcaa cctttgttat ggttgtatct gtccttgatg
    10201 gtgattggtc tactcaatgt atttgccgtt agctttagtg atgatgtgaa attttttgac
    10261 ctctccttac cacatggaaa acaatttttg tggatgggaa tttcattggt tgttggattt
    10321 agcattctca cctttgaaag cagttttttt acaaagttcg ccatggtgtt atatggtgtt
    10381 actatagccg tattaattgt cacatttgta gtagccgtag acattaacgg cgcacggtca
    10441 tggttgcagg tgggttcgtt tcagtttcag cccgcggaat tcgggaagac aactacggca
    10501 ctgatgctcg cgaaattttt gagtatcata aatggtcagc cgcgatcctt taaaaataaa
    10561 ctactgagct atctcatcat tggagtgccg atggggatta tcgttctgca aagcgatgtg
    10621 gggtctgcat tggtttatgc cagtttagta ttggttgttt atcgcgaagg atttccggtg
    10681 tatgaggtgt tgtttgtgct tgccattgga ttgtgtttcg tgttcgcact aatttttggc
    10741 gtagatccgg tggcggttgt gcttacatta atcatttggc tatacattgt atttagttca
    10801 ttcaaactgg tgcgaaaaga aaaaattaag ggaattgttt ttttagtgat tttttctgca
    10861 ggcgtattct tctttttgat aggtggcgaa gtattgggca tggatacaca tattgcagca
    10921 gcggttatgc tcattgccat gatagcggcg ctgattaatg tgagaaaggt tcgttttttt
    10981 caaatgaata ttccggtagt attatttgcc attttgatgg tatttgtgat gtttggtgta
    11041 gaaactatca caacagatat tcttgaagat tatcaggtgc aacgtatcga aacattattg
    11101 ggaatgtctg atgatgccga tgcggaatat aatgttactc aaagtaaaat gacaatcggt
    11161 tcgggaggcg tttttggtaa aggatacctt caaggcactc tcacccaatc tgaacaagtg
    11221 ccggagcaaa gcaccgattt tattttctgc actataggtg aagagtgggg tttctttggg
    11281 acattcattt ttcttgtgat ctatttgcta tttattctcc gcgtaatttt tatcgccgag
    11341 cgacaacgat cgcctttttc gagggtgtat gcttattgcg tggcaagttt tttcttcctg
    11401 caaatcatga ttaatgtggg aatgaccatt gggttaattc cggtaatcgg tatcccgctg
    11461 ccctttatct cctacggcgg atcttcggtg ctggctttca gtatcatgat attcatcatg
    11521 ctccggttgg atgccgatag gctattcata ttgcgttaag taccgaaata aggcttctga
    11581 ttgtcatacc gggctgcaag cacagttgcc gtgaattgca atatctttgc acaaaattca
    11641 catcaatgga aaccttccat atcggcatac aaggactcac gcttcaacaa ataagcgata
    11701 tcatcaaaaa taaaatacgc attgcattaa gcgatgaggc gcgtgagaaa attcagaaat
    11761 gtcgtactta tctcgataat aaaattgcaa gtcaggataa ggcaatttat ggtgttaata
    11821 ccggcttcgg aagtttgtgt aaagtgaaaa tttctgatag cgatctggag caactacaat
    11881 ataatttaat tcgcagccac gcttgtggaa tgggtgccgt tattccggat gaaatttgta
    11941 ggattttatt gctccttaaa atccaaaacc tggctttcgg ccattcgggt gttgccgttg
    12001 aaacggtatg gcgactgatt gatttttata acatggatct cctgcctgtt atctatgaac
    12061 aaggttcatt aggtgcatcg ggcgatcttt gtccgcttgc acatatgagt ttgccattga
    12121 ttggtgaagg agaattgaaa gtaaacggta aaatcagacc tactgcagag gtattgaaag
    12181 aagcgaaatt gtcgccattg aaattgcaat caaaggaagg tcttgcacta ttaaacggaa
    12241 cacagtttat ggctgcgcat gccgtgtata cattgattaa agctgataat attattcgtc
    12301 agtcgacata cattgccgga gtttctctcg aagcatacga ttgcaaatcg gagccgttta
    12361 ccgatcagat tcatcaaatt cgcgggcagc atggacagat tgctattgcg aaggctttta
    12421 tgcatataat gcagggttct gaattgtttt tcgctgagaa acagcaagtg caggatccgt
    12481 attctttcag gtgtattccg caggtgcatg gtgcttgtta tgataccata caatattgta
    12541 aaactattgt tgaacgcgag attaatggtg taacggataa tccgaatgta tttgttgagg
    12601 aggatatgat tttgagtggc ggaaattttc acggagagcc gctcgcttta actttagatt
    12661 ttctggctat ggcattgagt gagatgggca atatttctga acgaagggtt tatagattat
    12721 taagtggagc gcgtggactg cctgaatttt tgacgcctga tccgggattg cattcgggat
    12781 tgatgattcc gcaatatgct gcggcgagta ttgtttctca aaataaacag ttatgtactc
    12841 cggcgagtgt tgattctatt agcagttgta atgaacagga ggatcatgtg agcatgggtg
    12901 ccaatgcggc aacaaaatgt ttgcgtgtgg caattaatgt tgaacgtatt ctggctattg
    12961 aattgttgac tgcagcgcaa gctttcgaat tccgtcgccc tgctaaaaca tctccggcac
    13021 tggaaaatta cattgcttca taccgccaac acgtaacctt caacaccgaa gaccgcttcc
    13081 tccacaaaga catgctcact acagagaagt acttacaaat ggtgtgataa tttcacgcag
    13141 aggcgcagag acgcagagtt tttttgaaca tgttgtattg tggagcgaca atagcgtaag
    13201 cgcttagggt ggataaagtt ttattaaatt gctttttaat tggcaaaatt tggtgattgt
    13261 ttcaccctta aattttgtta tgcatattaa tcagctttcc ggtgttatcg tggatatttt
    13321 aatccacatg cacaaagaac ttggccccgg tttattcgaa tcagtttatg aagaggtgct
    13381 ggcatctgaa cttgataaac gtgagattgg gtataggcgg cagcaggtaa ttcctattgt
    13441 ttatgatgat gtagtgttag tagaaaatgc cttcaaggca gatattattg ttgataatag
    13501 gattataatt gaacttaaat ctatagatca attatcaccg gttcattata agcagttgtt
    13561 gacatattta cggctcacag gaataaagct cggcctgctt gttaatttca atgaagtcct
    13621 aataaaagat ggcattcaca gagtagtgaa taatctctaa actctgcgtc tctgcgcctc
    13681 tgcgtgaaat cttacagtag cttaacccca acaatacgga gctgatcgtt gtcgacttcg
    13741 ccgggggcgt ccatcatcat atcgcggccg ttgttgtttt ttgggaaagg aattacatcg
    13801 cggatagtat ctccacccgc catgagcatt acccatctat cgaagccaaa cgcacaacca
    13861 ccatgcggcg gtgcaccata tttaaaagca ttcaccataa atccgaagcg acgttctttt
    13921 tcttcctcag agaagccaag aatcttgaat attttttcct gaagttcttt gttgtgaata
    13981 cgaatactgc cactcaaaat ctcattgcca ttcatcacca tatcataact ctgagcacgc
    14041 acgcgcaaag gatcgctatc catatattgg aaatcatcgg gatgcggcga gcagaatgga
    14101 tgatgcgcaa aaatcggttc acccgtttct tcatcaggtt cgaataaagg catatctaca
    14161 acccaaaata cactccacgc atttttatcg atccaatttt cacgcttcgc catttccaat
    14221 cgcagatcgc cgagtacttt gcgtgttttc ttttctttat cggcaactat cagtaataaa
    14281 tctcctttgc ctgcaccaac tgattcgccg atagctatta attgttcagg tgtaaagaat
    14341 ttatccatgg atgattttac ggagccatcc tgactgaatt taatatatat caaaccaccg
    14401 actccacgct gcggcgcttt aacaaattcc gtgagttcgt cgatttgttt gcgtgaatat
    14461 tcactgcatc cttttgcaac aatacccgca accaatccat tggaagccag cacattatta
    14521 aatacaccaa attcacttcc gcctacaacg gcatttaagt cttgaatttt acaatcgaag
    14581 cgcaaatccg gtttatcgct gccgtaatat ttcatcgcat cacggtaagg caagcgaata
    14641 aaatccggaa ggtcaatatt ttttactttc ttaaatacat gcttcgtcat tccttcaaac
    14701 atattccaca catcctcctg acgcacaaag ctcatttcac agtcgatctg cgtaaattcc
    14761 ggctgacggt cgccacgcaa atcttcatcg cgaaaacatt ttgcaatttg gtagtagcga
    14821 tccattccgc ttaccataag caattgtttt aatatttgcg gcgattgcgg caatgcataa
    14881 aacaatcccg gctgcatgcg cgaaggcact aaaaagtcgc gcgcaccttc gggagtggat
    14941 tttatcaggt ttggtgtttc tacttcaaca aacccttcag catctagata ctcgcgaacg
    15001 gctttgagca tatttgcacg aagcatcaat cgctgttgca tctgcgggcg acgcaaatcg
    15061 agataacgat agcgcatacg caattcttca ccgccatccg tttcatcatc catagtaaat
    15121 ggaggaagct cagctgcatt gagaatttcc agtttgtcga caataatctc gatatcacct
    15181 gttggcaatt gcgcattttt gctggaacgt tcagttacaa caccgctggc gcagatcaca
    15241 aattctcttc ccagcttgcg cgccatttcg cataaggcag catttgattc catattgaac
    15301 accaattgcg tgattccgtg acggtcgcgg aggtcgatga aggtcatgcc tccaaggtcg
    15361 cggctacggt gcatccatcc cgaaagggta actgtagtgc caacggattg cattctgagc
    15421 tctccgcagg tatgggtgcg ataaaaggtg ttactatgca tattcgaaat ttgctgcaaa
    15481 aatacggaaa ggtggggtgg gataaaaacg ctgactcttg taaggacgcg gatttgcgta
    15541 tttttgagcg acatagtcac tacgtatcat gcattgtgtt tcctgcggac atactcattt
    15601 cagtgccacc ccttattatt atcaatggaa tagtaaggaa tttgtactca ctacatgcaa
    15661 gaattgcggt ctcatcacca tggatcccaa acccaccgat gaggagctgg gattgttgta
    15721 tgccgatgaa tattttgaaa ccggtcagca tggattaaac cgcattggca tgacctatga
    15781 gcaaggtcag gataaaaacg ggatagatca ccgcatcaat gcagttaagc aaaatatgct
    15841 cgcgtttaaa cctgacatgc aaagcgtttt cgaaatcgga tttgctatgg gacatatgct
    15901 ggcagccgca cgtgatcttg gacttgaaat tggtggaata gaattttcgc ctatggcagt
    15961 gagtcgcgcg aaggaaaaat ttaatattga tgctttgtgt ggcaattttg agaacatcga
    16021 cctgtcggca catctacata aatgggattg tgtatacggt ggcgatgtat tcgaacattt
    16081 tattca
//
LOCUS       DAOQHY010000069        15944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1732_length_15944_cov_5.323494, whole genome shotgun sequence.
ACCESSION   DAOQHY010000069 DAOQHY010000000
VERSION     DAOQHY010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15944
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1732_length_15944_cov_5.323494"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PLL28_07325"
     CDS             <1..345
                     /locus_tag="PLL28_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNF69171.1"
                     /translation="CFPEIASVGMTEAAAKAAGYELKIGKFPFTASGKAKAGGTPEGF
                     VKVIFDAKYGEFLGAHLIGANVTEMIAEVVAARKLETTGHEIIKTIHPHPTMSEAIME
                     AAAAAYGEVIHM"
     gene            complement(626..1654)
                     /locus_tag="PLL28_07330"
     CDS             complement(626..1654)
                     /locus_tag="PLL28_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HNF69172.1"
                     /translation="MALQVYSADDIISRTKRRHGETKIGERIQTITNADKWQDDLATS
                     TASFVVMGIPEDIGVRANHGRGGAYAAWKPSLDFLINMQSNHFLDGSEMLVLGHVIVD
                     DLMQQADKLDYKREEDIQAARRLVEQVDNRVEHIIHSIVAAGKIPIVVGGGHNNAYPL
                     IKGTSKACGNPIHVINCDPHSDMRPLQGRHSGNGFHYAWHEQLMSKYAVFGLHESYNI
                     YSALELFAQNPEYLTFETYDDIFVRESVNFFDALKRCMDFVDGAPCGIEIDLDSIQNV
                     PVSARTPSGMLPVDVRKFVFHCGENLPAKYLHIAEGAPVLAHKQVDNKTGKLVAYLIT
                     DFVKGKVN"
     gene            1749..2288
                     /locus_tag="PLL28_07335"
     CDS             1749..2288
                     /locus_tag="PLL28_07335"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNF69173.1"
                     /translation="MNQKAHITKTALSLFAAQGYGNTSVAQIAKQAGVSQGLMYNFFS
                     SKDALLEAILAEGMSDIANSMLEYQNGAAPKDALRAHIKNTFRIVSENNAYWRLFHSI
                     RMQEPVRTLLGERYAAIQKEILHTLTATFRKLSYEHPKQEARIFFALIDGLVGHYLLD
                     PSQFQLSLIQKTLFKKYQL"
     gene            2285..3145
                     /locus_tag="PLL28_07340"
     CDS             2285..3145
                     /locus_tag="PLL28_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNF69174.1"
                     /translation="MTPEWLNSNQYPFAPKYLSLPNAQMHYIDEGSGEVILFVHGTPS
                     WSYEFRDAIRILSRNYRCIAMDHIGFGLSDKPADYSYTLDKHKENLMSLIAHLQLSQF
                     NLLVHDFGGPIGLSVATSMPEKINKIIMANTWCFSVADEPEFKRMKAILGSPLLPFLY
                     RNFNFSARYIIPAAFGERTRLTPEIHAQYMKPFAKRSSRNGTIGFAKSLLKDQAWFEN
                     VYRQIGVLVNKPALLLWGLKDNFITEKYLHRMQEIFPNAEVVQYADAGHFVFEEKGSV
                     AAVAIERFLA"
     gene            3388..3921
                     /locus_tag="PLL28_07345"
     CDS             3388..3921
                     /locus_tag="PLL28_07345"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNF69175.1"
                     /translation="MIEHQHSNCFFITFNCRNHFHLLKSDVAKQMVIDSLLRYSEIYQ
                     VSLLGYVIMPNHVHMIILFSGRVRLSDFMRDIKKYSSVLIRDFLLLNHPGETFKINFI
                     QREQLHKIWQDGYHRKVVFTARFLKQKLNYIHRNPLQEHWKLVEAPELYKYSSANYYY
                     ANKQESEIVTHFGDFFR"
     gene            complement(3980..5890)
                     /gene="ftsZ"
                     /locus_tag="PLL28_07350"
     CDS             complement(3980..5890)
                     /gene="ftsZ"
                     /locus_tag="PLL28_07350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNF69176.1"
                     /translation="MYFDIPKEQASIIKVIGVGGGGSNAVNHMFLQGIKDVNFVICNT
                     DQQALEMSPVPNKVQLGPSLTKGRGAGSHPTVGQQATMESLQEIKSILEKNTEMVFIT
                     AGMGGGTGTGGAPVIAKLAKDMGILTIGIVTVPFGFEGKRRKSQAFEGLETLKHYVDA
                     ILVISNDKLREMFGNLAWNEAFSQADDVLATAAKGIAEIITVPGYVNVDFEDVKTVLR
                     GSGLAIMGSGKAAGQDRALEAVRVALESPLLNDNDIRGARSILLNITSGTTPVLMDEI
                     AEITEFVQEAAGNDCDIIWGNCNDESLGESIMVTLIATGFETVEQRNQRIKQTTVKVT
                     HVNTHELLQKKVTPIEEPIAEVKEPEAEPEAVITLKHEPKEDKKAKKEEVKETKEVKE
                     QNPSQFSFDFGLFANTIQEKPVINEVPPVVEPPVAKIEEVSFEEKEELVIELKKQDPI
                     TEIISEQEEETAEEEVMSPVEMHLRPSVIIEPVNEFEEPVAEEEALDMPMMNEVEEET
                     IFEIKTNIRHEAIEPEAEIEEEQIMIRKEEQRVSQKQEGRSFITNDDKINVVNSTGND
                     RINRLKSMSMKLSGNTLDEVEKVPAFMRRNIELEDTPNAREINVSKYNIVEGEDGPEL
                     KKNNSFLHDNVD"
     gene            complement(5954..7360)
                     /gene="ftsA"
                     /locus_tag="PLL28_07355"
     CDS             complement(5954..7360)
                     /gene="ftsA"
                     /locus_tag="PLL28_07355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNF69177.1"
                     /translation="MNNSNRIITGLDIGTTKICAIVGKITDHGKIEILGMGKADSFGV
                     MRGVVANIDKTVEAIRRAVAEAQEKSGVEINEVYVGIAGQHIKSLQHRDILTRNDAES
                     EIGMDDIDRMIENMHRLVLPPGDRIIHVLPQEFIVDGEQGIKDPIGMSGVRLEANFHI
                     ITGQISAAKNIFKCVEKAGLKVIDLILEPLASSASVLSEEEKEAGVALVDIGGGTTDI
                     AIFQDGIIRHTAVIPLGGNIITEDIKEGCMIMRNQAEILKVKFGSALATETEENEIIS
                     ISGLKGREPKEIKVENLARIIQARVEEILEHVYYEIKLSGFHKKLIGGIVITGGGAQL
                     KHLVQLVSYITGMDARVGYPTEYLQKAKAEDIRHPMYATAVGLILKGHDDRNYREREI
                     EMPAPQQQQEIVNPVVDKMPTSNEQLNQVAEKEMNDATMEMEQELAHDKHAKPSWLKN
                     MLSSTRKWFEEDDVSDFK"
     gene            complement(7347..8123)
                     /locus_tag="PLL28_07360"
     CDS             complement(7347..8123)
                     /locus_tag="PLL28_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69178.1"
                     /translation="MRVFGILLLVACGTGLLLLTAFNADRQDASACIDVQIHVDEERG
                     IYFLDANDIRTILATEYGDSVKGDALGSIRVGRIETLLERNPYVDDAETWVDATGKLH
                     IEIVQKQPIARVINKYGVNYYINNIGNKIPISDKFTARVPVVTGSIEEGIHNADMATT
                     SALQNILKITRFINADPFWSAQIEQIAVANDGTFTLIPKLGDHKILFGGIAHMEEKFR
                     KMQVFYTEGLNHTGWNTYSAFQLDFEGQIVCEKNKNYEQQ"
     gene            complement(8141..9487)
                     /gene="murC"
                     /locus_tag="PLL28_07365"
     CDS             complement(8141..9487)
                     /gene="murC"
                     /locus_tag="PLL28_07365"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNF69179.1"
                     /translation="MDIAQINRVYFIGIGGIGMSALARYFMRQKCVVSGYDRTETEIT
                     DALQHEGAQIHFSEDLTQIDTQADLVIYTPAVPPTHAELIYYRDNGFTVLKRSQVLGL
                     ITKDKFVIAVAGSHGKTTVSSMIAWILKNSGYDCTAILGGISTNFNSNYVMGFNDVFV
                     VEADEYDRSFLQLSPNIAVITATDSDHLEVYGTQEAIEATFLEFANRIKENGLLISKP
                     RLGVFQKYQRDKRGYDLTDSKADLYAVTYAYEGDRCHVTLNDGYEFYLGYPGVHNIEN
                     AIAAISVALHLNIHPDLISRAITAFTGVHRRFELVNGVFGKIPYYDDYAHHPEEIKMF
                     LTSIKQLFPNKKITAVFQPHLFTRTRDLADGFATSLDIADEVILLPIYPAREEPIPGV
                     TANNILDRMKNPHRRILEKADLIKYVSKADIEVFCTIGAGDIDKLVQPIAEILKGR"
     gene            complement(9491..10594)
                     /gene="murG"
                     /locus_tag="PLL28_07370"
     CDS             complement(9491..10594)
                     /gene="murG"
                     /locus_tag="PLL28_07370"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNF69180.1"
                     /translation="MASSINILISGGGTGGHIFPAIAIANEIKRRRPDANILFVGAQD
                     KMEMEKVPKSGYPIEGLWISGFHRGLDWRNLMFPIKLISSYFKSGSIVKKFEPDIAIG
                     TGGFASGPALNAALRHQVPIIIQEQNSYPGVTNKILSKKAKAVCVAYDGMEQFFPAGK
                     IVKTGNPVRQNIVLCNVKPEKARADFDIDIDSKVLFVVGGSLGSRTLNNCMIGGLQEL
                     ADAGIQVIWQTGTAMYEMCKHAAKGYDNVRVLDFIANIDHAYAAADIIVSRAGAIAIS
                     ELCLVGKPVILVPFPAAAEDHQTKNAMALVKENAAMHIADKSAPVELIPNAIQLMQDH
                     DKRSLMAQRIKNFAIADAAERIVDEVFKYVEAK"
     gene            complement(10612..11823)
                     /locus_tag="PLL28_07375"
     CDS             complement(10612..11823)
                     /locus_tag="PLL28_07375"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HNF69181.1"
                     /translation="MSNSIQYLFSRVRGDRYIWLVVFVLSVFSILAVYSSTETLAYRS
                     AGGNTEYYLVKHSVILLFGIVLMYLSHLIPYKYYSRIAQILLWLSVPALLYTMFLAPE
                     VNDASRWITLPVINLTFQTSDLARFALIMYTARVLSKKQDKVDSFKEAFIPVILPIAI
                     ICALILPENLSTASVLFFTCIVLMFVGRVKFKYILLTIGAGIVLIGLIIALAYAFPHV
                     GRMGTWKARVDSFVDGGTSDDTYQVDQAKIAIAKGGVFGVGPGNSTQKDFLPYPYADF
                     IFATILEEYGLMGGIFVIFLYLVFLYRCIKIVVKAPTSFGALLAVGLGLCLCIQAFIN
                     MGVAVHLLPTTGLTLPLVSMGGTSLWFTSLSIGIILSVSREIEKADEVEEEDEQTETE
                     EQSEEEPELAK"
     gene            complement(11863..13215)
                     /gene="murD"
                     /locus_tag="PLL28_07380"
     CDS             complement(11863..13215)
                     /gene="murD"
                     /locus_tag="PLL28_07380"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNF69182.1"
                     /translation="MAKKLVILGSGESGTGAAILAQQQGFEVFVSDKGKIRDSYKHEL
                     SSRGIAFEEGKHSMDIILAADEIIKSPGIQEKYDVMKQIRAANKIVVGEIEFAWRYCK
                     GKVVAITGSNGKSTCTSLTYHMMKKAGCDVAIGGNIGKSFAELVAHTNHEYYVLEVSN
                     FQLDDAVTFKPMISVLLNITPDHLDSYNYEFQKYIDSKFRITMNQTDGDHFIYCADDP
                     VTSEEMLKRHSPFLSNPANLLTFTLDDRQHEGAWVADNSIHIQINKNHFTMTINELAL
                     QGKHNQYNSMAAGIVGKVLGLKNESIRESFTDFSGLEHRLEFVAKVHGISFINDSKAT
                     NINSTWYALESQADPVIWIVGGIDKGNDYSLIADLVKKKVKAIVCLGKDNTRIHEAFG
                     KMVDILVNTETMEDCVQMAYRLGKNGDIVLLSPACASFDLFENFEDRGNQFKKQVRLL
                     "
     gene            complement(13215..14519)
                     /gene="mraY"
                     /locus_tag="PLL28_07385"
     CDS             complement(13215..14519)
                     /gene="mraY"
                     /locus_tag="PLL28_07385"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNF69183.1"
                     /translation="MLYYLFNWLDQQYDVPGAGMFQYLSFRAGLAVLLSLIFSIVIGK
                     RLIAYLQKKQIGESVRDLGLEGQLSKKGTPTMGGIIIILSLLIPVLLLADLKNIYVIL
                     MLIVTVWMGLIGFLDDYIKVFRKNKEGLRAKFKLMGQVGLGAIVGLVLYFSDDIKLRD
                     YDMVYVYAVENGQPVGRPIDAYPANDTRLESDGILNSDSTHFLSHDTRSTKSTIPLVK
                     NNEFDYEWLVSWASPETQKWLFPIVFVAIIIIIITAVSNGANLTDGLDGLAAGTSTII
                     VLTLLIFAYIAGNSILSDYLNIMYIPDSGELVVFSAALIGACIGFLWYNAFPAQVFMG
                     DTGSLTLGGIIASLAIVIRKELLIPVLCGIFLVENLSVMIQVSYFKYTRKKYGEGRRI
                     FLMSPLHHHYQKKGMFETKIVTRFWIITILLAIVSIVTLKVR"
     gene            complement(14519..>15944)
                     /locus_tag="PLL28_07390"
     CDS             complement(14519..>15944)
                     /locus_tag="PLL28_07390"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNF69184.1"
                     /translation="QHGVSLTSVQIIGITADSRRAAEGMLFVAVKGTTVDGYDFIPTA
                     IAQGAVAIVAERKIEVPDNVCLIIVDNAAAALGIMAHAFYDYPSTKLKLVGITGTNGK
                     TTTATLCYHVMRDLGFNTGLISTVENKINEEVIPSTHTTPDPVQLNALLAKMVDAGCA
                     YAFMEVSSHAAHQHRIAGLQFVGGVFTNITHDHLDYHKTFKDYIDAKKMFFDGLTSDA
                     FALVNSDDRNGKIMLQNCKAKHATFSVLGTGDFTARIKENVISGLVLQLDGVEFHSLL
                     LGEFNAWNILTVYAIFRLLGFEKQQVLTVLSAQKPVEGRFDVLYTGDRSITGVIDYAH
                     TPDAVEKLLSTVRSMLKKDQHLLTVVGCGGDRDRGKRPVMAATAAKISDKTILTSDNP
                     RSEEPDAIIKEMESGLNEETRKHSLSITDRKEAIRTAVMLAKKGDVICVVGKGHEKYQ
                     EIKGVKYPFDDKEVLRETFKTLAH"
BASE COUNT         4390 a         3606 c         3102 g         4846 t
ORIGIN      
        1 tgcttccctg aaattgcaag tgtgggcatg actgaggctg ctgcaaaggc tgcgggatat
       61 gaattgaaga tcggaaaatt cccattcaca gcttccggta aagcaaaagc aggtggtacg
      121 cctgaaggat ttgtgaaggt aattttcgat gctaaatacg gcgaattcct tggcgcacat
      181 ctcattggcg ccaatgtaac ggaaatgatc gcagaagttg ttgctgcccg taaactagaa
      241 actacaggtc acgagatcat taaaacaatt cacccgcacc caaccatgag cgaagctatt
      301 atggaagcag cggcggcggc gtatggtgag gtgatacaca tgtgatgagt gggtgagtgc
      361 gtgagtggtg agcctgcctc gccgataggc gggtggtgag tctgccttat tgatgacagc
      421 cggatgagtt tattttgttt gacgtgctta gattaataat ccatttggtt gatgaatagt
      481 aatcaggatg ccctccgatg ccttttctgt tgttctcaaa cttacacaat ccaataattg
      541 cacactcgca catggagcgc agcgacatcc cgcctcagcg ggatcatcac atttccatcg
      601 gctaatcggc taattggcta atcggctaat taaccttccc tttcacaaaa tccgtaatga
      661 ggtaagctac tagttttcct gttttattat ctacctgttt atgggcgaga accggtgccc
      721 cttcagcgat atggagatat tttgccggga gattttctcc acagtgaaat acaaattttc
      781 ttacgtcaac aggaagcatc ccacttggag ttcgtgcgct aacaggaaca ttttgtattg
      841 aatccaaatc gatctcaata ccgcatggag cgccatccac aaaatccatg cagcgcttta
      901 atgcatcgaa aaaatttaca gactcacgta cgaatatgtc gtcatatgtt tcaaaggtga
      961 gatactcggg attttgagca aataattcca gtgccgaata aatattatac gattcgtgta
     1021 atccgaatac ggcgtatttc gacattaatt gttcatgcca ggcataatgg aatccatttc
     1081 ctgaatgtcg cccttgcaat ggacgcatat cactatgcgg atcgcaattt ataacgtgaa
     1141 tgggattgcc gcatgctttg gaagttcctt tgatgagcgg ataggcattg ttgtgacctc
     1201 caccaacaac tattggtatt tttccggctg ctacaattga atgaataata tgttcaacgc
     1261 gattatccac ttgttctaca aggcgacgcg ctgcttgaat atcttcttct cgcttataat
     1321 caagcttatc tgcctgctgc ataaggtcat ctactatcac gtgaccgagc acgagcattt
     1381 cactgccatc taaaaagtga ttgctttgca tgtttatcaa aaagtcgaga ctcggcttcc
     1441 atgccgcata tgcccctccc ctgccatgat tagctcgaac gccgatatcc tccggtattc
     1501 ccatcaccac aaaactagct gttgatgtcg ccaggtcgtc ctgccactta tctgcattag
     1561 tgatggtctg aatgcgttcg ccaatttttg tttcgccatg tcggcgcttg gtgcgggaaa
     1621 tgatatcgtc cgccgagtaa acctgcaatg ccatgataca aaggtaggtc tttatgcaaa
     1681 acagatgatg acacccggtt ttgaaatttg gaaatgtacc ttacctttgt gaatgaacgt
     1741 tcattcatat gaaccaaaaa gcacatatca ccaaaacagc cttatcgctc tttgccgcac
     1801 aaggttatgg aaatacctcg gtggcacaga tcgccaaaca ggcgggggtg tcgcagggac
     1861 tgatgtataa ttttttttca tcaaaagatg cccttctcga agcgatactg gccgaaggaa
     1921 tgtcagacat cgccaattca atgttggaat accaaaacgg agcggcaccc aaagatgcct
     1981 tgcgggcaca tattaaaaat acctttcgca tagtatcaga aaataatgct tactggcggt
     2041 tgtttcactc catcaggatg caggaaccgg tgcgcacatt gcttggtgaa cgatatgctg
     2101 ccatacaaaa agagatatta cataccctca ccgccacctt ccgcaaatta tcatacgaac
     2161 atccgaaaca ggaagcaaga atattcttcg cactgataga tggcttagtt gggcattacc
     2221 tactcgatcc ttctcaattt cagttatcac ttattcaaaa aacgcttttt aaaaaatatc
     2281 aattatgact cccgaatggc ttaattcaaa ccaatatccc ttcgcaccaa aatatttatc
     2341 gctgccaaat gctcaaatgc attatatcga tgaaggctcc ggagaggtaa tcttatttgt
     2401 gcacggcacg ccttcatgga gttatgagtt tcgcgatgcc atcaggatac tttccagaaa
     2461 ttatcgctgc attgcaatgg atcatatcgg ctttggtttg tccgataaac ctgccgatta
     2521 cagctataca ctcgataagc acaaagaaaa tttaatgtca ctcattgcac atttacagct
     2581 cagccaattt aatttactgg tgcacgactt tggtggtccg atagggcttt ctgttgctac
     2641 aagtatgcct gaaaagatca acaaaatcat catggccaat acatggtgct tcagtgttgc
     2701 cgatgagccc gaatttaaac gcatgaaggc aattcttggc agtccgctgt tgccgttttt
     2761 atatcgcaat ttcaatttca gcgcgcgtta tattatacct gcagcatttg gcgaacgaac
     2821 cagactgacg ccggaaattc atgcacagta tatgaaacca tttgctaaac gttcatcgcg
     2881 aaatggcact atcggttttg ctaaatcgct gttgaaagat caggcgtggt tcgaaaatgt
     2941 atatcgacaa attggtgtac ttgtcaataa gcctgcttta ctattatggg gattgaaaga
     3001 caatttcatt accgagaaat atttacatcg catgcaagaa attttcccga atgcagaggt
     3061 agtgcaatat gccgatgcag gacactttgt tttcgaagaa aagggctcgg tggctgcagt
     3121 tgcaattgag cgtttcctag cctagggtcg gatgctagca tccgacccta ggctaggaaa
     3181 tccccatgta tgtaaaatct caaaaccaaa tgcgattttt ccacattcta aactgagtaa
     3241 ttttcatccc caccatttct cactaaccgc ttattaagtt gcctgccgac atcaattgat
     3301 aaatgtattt ttcagagtct catcggtgat cggattattc acaattcagc ctacaatacg
     3361 gaattattca tgtgctcata ttagcatatg attgaacatc aacattccaa ttgttttttt
     3421 atcaccttca actgccgaaa ccactttcat ttgcttaaaa gcgatgtagc taagcaaatg
     3481 gtgattgata gtttattgcg ctacagcgaa atatatcaag tcagtctctt agggtatgta
     3541 atcatgccaa accatgtgca tatgataatc ctattttcag gcagagtgcg gctatcagat
     3601 tttatgcggg atattaaaaa atattcaagt gtactgatcc gcgattttct gttattgaat
     3661 caccccggtg aaacctttaa gattaatttt attcaaaggg agcagctgca taaaatatgg
     3721 caggatggtt atcacagaaa agtagtattc acggccagat ttctgaaaca gaaattgaac
     3781 tacatccata ggaatccatt acaggaacat tggaaacttg ttgaagcgcc ggagctatac
     3841 aaatattcca gtgcaaatta ttattatgcc aacaaacaag aaagcgagat agtgacacac
     3901 tttggcgatt tctttcgata aacggagcgg gcaaaaaaaa acaggccatg tggcctgctt
     3961 tttttgttat gacgtataat caatctacat tgtcgtgcaa gaaggagtta ttctttttta
     4021 actccgggcc atcttcgcct tcaacgatat tgtatttcga cacattgatt tcacgagcgt
     4081 ttggcgtgtc ttccagttcg atatttctgc gcataaaggc aggaactttt tctacctcat
     4141 caagtgtatt tcccgataat ttcatgctca tacttttcaa gcggttgatg cgatcattgc
     4201 cggtgctgtt tactacattg attttatcat cgttggtaat gaaactgcga ccctcttgtt
     4261 tttgagaaac gcgttgttcc tcttttcgaa tcatgatctg ctcttcctcg atctccgctt
     4321 caggttcaat tgcctcgtga cgaatatttg ttttaatctc aaatatcgtc tcttcttcta
     4381 cttcattcat catcggcata tccaatgctt cttcttcggc aaccggttct tcaaactcgt
     4441 tgacaggttc tatgatcact gatggacgaa gatgcatttc tacaggcgac atcacttcct
     4501 cttcagcagt ttcctcttcc tgttcagaaa taatctcagt gatcgggtcc tgcttcttca
     4561 actcgatcac taattcctcc ttttcttcaa atgaaacttc ttcaattttt gctacaggag
     4621 gttcaaccac aggcggcact tcattgatca ccggtttttc ctgaatagta ttggcaaaca
     4681 aaccgaagtc gaagctgaat tgcgaagggt tttgctcctt cacctccttg gtttctttta
     4741 cttcttcttt ctttgctttt ttatcttctt taggctcgtg ttttaatgtg atcacagcct
     4801 ccggttcagc ttcaggttcc tttacttccg ctattggttc ttctatcggc gttacttttt
     4861 tttggagtaa ttcatgtgta ttcacatgcg taaccttcac tgtggtttgt ttaatgcgtt
     4921 gattgcgctg ttcaacagtc tcgaatcctg tagcaatcaa tgtcaccata atagattcac
     4981 ccaatgattc atcattacag ttaccccaaa taatatcgca atcatttcct gcggcttctt
     5041 gcacaaattc agtgatctct gctatttcat ccatcaacac aggcgtagtt cccgaggtga
     5101 tatttaacaa aatgctgcgt gcaccgcgaa tatcattatc gttcaacagt ggtgattcaa
     5161 gtgcaactcg tacagcttca agtgcgcgat cctgacctgc tgcttttccg ctacccataa
     5221 tggcaagacc acttccacgc aatactgttt ttacgtcttc aaaatccacg ttcacataac
     5281 cgggaacagt aataatttcg gcaatacctt ttgcagctgt tgccaataca tcatcagcct
     5341 gactaaatgc ttcgttccaa gccaggttac cgaacatttc gcgaagctta tcattcgaga
     5401 tcaccaaaat ggcatctaca taatgtttga gcgtttcaag gccttcgaat gcttgcgatt
     5461 ttctgcgctt tccttcaaaa ccaaatggca ctgttacaat accgatggta aggatcccca
     5521 tatctttagc aagtttcgct ataacgggag caccacctgt tccggtaccg ccacccatac
     5581 cggcagtaat aaaaaccatc tcggtatttt tttcgagaat agattttatt tcctgcaagc
     5641 tttccatggt agcttgttgg ccgacagtag gatgtgaacc tgcaccacga cctttggtaa
     5701 ggcttggtcc aagctgcact ttattgggaa cagggctcat ttcaagtgcc tgttggtcgg
     5761 tattgcagat tacaaagttc acatctttga tgccctgcaa aaacatgtgg ttcacggcat
     5821 tgctaccgcc accgcctact ccgatcactt taatgataga ggcttgttct tttggaatat
     5881 caaaatacat aggttcgttt tcgttttatt gtgtgttgtg tttcaaggta ttgcccgcgt
     5941 tcacgttcgg gtattattta aaatcagata cgtcgtcttc ttcaaaccac ttacgtgtgc
     6001 tcgacaacat gtttttcaac caactcggtt tagcatgttt atcgtgcgca agctcttgtt
     6061 ccatttccat ggttgcatca ttcatttcct tctctgcaac ttgattgagc tgctcattgc
     6121 ttgtcggcat tttgtcaaca acaggattca cgatttcctg ttgctgctga ggtgccggca
     6181 tttcaatttc gcgctcacga taattgcgat cgtcatgacc ttttaagatc aagccgacag
     6241 ccgttgcata cattgggtga cgaatatctt cagcttttgc tttttgcaag tattctgtcg
     6301 gatatccaac gcgcgcatcc atccctgtga tataggatac cagttgcacg aggtgtttga
     6361 gttgagcacc accaccggtg attacaatac caccaatcag ctttttgtgg aagcctgata
     6421 atttaatttc atagtaaacg tgttcgagaa tttcttctac acgcgcttga atgatgcgtg
     6481 caagattttc aactttgatc tcttttggtt cgcgaccttt caatccgctg attgagatga
     6541 tctcattctc ttcagtttct gttgccagtg ccgatccgaa tttcactttc aggatttctg
     6601 cctgattgcg catgatcatg caaccttctt tgatatcttc agtgatgata ttaccaccca
     6661 gcgggatcac ggcagtatgg cgaatgatac catcctggaa aatggcgata tctgttgttc
     6721 caccgccaat atcaaccaag gcaacaccgg cttccttttc ttcttcgcta agtacggaag
     6781 cagaagatgc caatggttca aggatgaggt ctattacttt gagaccggct ttctctacac
     6841 atttgaaaat atttttcgct gcagaaatct gaccggtgat aatgtgaaaa tttgcttcaa
     6901 gacgcacacc actcataccg ataggatctt tgataccttg ttcgccatcg acaataaatt
     6961 cttgcggcaa cacatggatg atgcgatctc cgggaggaag cacaaggcga tgcatgtttt
     7021 caatcatgcg atcgatatcg tccattccga tttcactttc tgcatcgttg cgcgtgagga
     7081 tgtcgcgatg ttgcagactt ttgatatgct gaccggcgat accaacgtaa acctcattga
     7141 tttcgacgcc acttttctct tgagcttcgg caacggctct gcggatagct tctaccgttt
     7201 tatcgatatt ggcaaccacg ccgcgcataa cgccgaatga atccgctttt cccatgccga
     7261 ggatctcgat ttttccatga tcggtgattt tacctacgat agcgcagatc ttcgttgttc
     7321 cgatgtcgag tccggtgatg atacggttac tgttgttcat agttcttgtt tttttcacaa
     7381 acgatctgcc cttcaaaatc caattgaaaa gcactgtatg tattccaacc ggtatgattc
     7441 aggccttcgg tataaaaaac ctgcattttc cggaattttt cttccatgtg tgcgatacct
     7501 ccaaacagta tcttgtggtc accaagctta gggatgaggg tgaaagtgcc gtcattggcg
     7561 acagcaattt gttcaatctg agcgctccag aatggatctg cgttaataaa acgcgtgatt
     7621 ttgagtatgt tttgtagcgc cgaggttgtt gccatgtctg cgttatgaat gccctcttct
     7681 atgctgccag taacgacagg tacacgggct gtaaatttat cgctgattgg tatcttatta
     7741 cctatattat taatataata attaacacca tacttgttaa taacgcgagc aataggttgt
     7801 ttttggacaa tttcgatatg caattttcct gttgcatcaa cccaggtttc agcatcatcg
     7861 acgtatggat tgcgttcgag taaagtttcg atgcgaccaa cacgtattga tccgagtgca
     7921 tcgcctttca ccgaatcgcc gtattctgtt gccaggatag tgcgtatatc attggcatcg
     7981 agaaaataaa taccgcgctc ttcatccaca tgaatttgca catcgataca ggcgcttgca
     8041 tcctgtctat cggcattgaa agccgttaac aacagcaatc ctgttccgca tgctaccagc
     8101 agcaggatac cgaaaacgcg cattatcttt tgttttccgg tcatcggcct tttaagatct
     8161 ctgcaatggg ttgaacaagt ttatcgatat caccggcacc gatagtacaa aacacttcga
     8221 tgtcggcctt ggaaacatat ttaatcaaat cagccttctc gagtatgcgg cgatgcgggt
     8281 tcttcatgcg gtcgagaatg ttattggcgg taacaccggg aattggttct tcacgtgccg
     8341 gataaatggg taataaaatc acctcatcag caatatctaa acttgtggca aatccatcag
     8401 caagatctcg tgttctggta aagagatgcg gctgaaatac tgccgtaatc tttttattcg
     8461 gaaataattg cttaatactg gtgagaaaca tttttatctc ctcagggtga tgcgcataat
     8521 cgtcgtaata tggaattttc ccaaaaactc cgttcactaa ttcaaaacga cgatgcacac
     8581 cggtaaaggc tgtaatagcg cgtgatatca agtccgggtg aatattcaaa tgtagtgcaa
     8641 cagatattgc ggcgatggca ttttcgatat tatgcactcc cggatatccc aagtaaaatt
     8701 catagccgtc attcaatgtg acatgacaac gatccccttc atatgcgtag gttacggcat
     8761 acaaatcggc tttactatct gtcaggtcat atccacgctt atcgcgctga tatttttgga
     8821 atacacccaa tcgaggtttc gaaatcagca atccattttc ttttatcctg ttggcaaatt
     8881 caagaaatgt tgcttctatt gcttcctggg taccatacac ttcaaggtga tcggagtctg
     8941 tagcagtgat cacagcaata tttggcgata attgcaaaaa ggatctgtca tattcatcgg
     9001 cttcaacaac aaaaacatcg ttaaaaccca tgacatagtt cgaattaaaa tttgttgaga
     9061 tacctccaag aatagcagtg caatcgtaac cactattttt caaaatccag gcaatcatac
     9121 tcgatacagt agttttacca tgcgagccgg ctactgcaat cacaaacttg tctttagtta
     9181 tcaaacccag aacctgactg cgtttcagca cggtaaatcc attgtcgcga tagtatatta
     9241 attcggcatg cgttggcgga actgcaggcg tataaattac gagatctgct tgggtgtcta
     9301 tttgtgtaag atcttcacta aaatgaattt gagcaccttc gtgttgcaat gcatcggtaa
     9361 tttctgtttc tgtgcgatca tatccagaca ctacacattt ttgtcgcata aagtaacgcg
     9421 caagtgcact cataccgatg ccaccgatac caataaaata aaccctattt atttgcgcga
     9481 tatccattat ttattttgct tcgacatatt taaatacttc atcaacaata cgctctgctg
     9541 catcagcaat tgcaaaattc ttaatgcgtt gtgccattaa cgaccgttta tcatgatctt
     9601 gcatcaattg aatggcgttt ggtattaatt ctacaggtgc cgatttatct gcaatatgca
     9661 tggcggcatt ttctttcact aacgccattg cattttttgt ttgatgatct tctgcagcag
     9721 caggaaatgg caccagaatt acaggtttcc ctacaaggca taattccgaa atggcaatag
     9781 cccctgctct tgaaactatg atatctgcgg cggcatatgc gtgatcgata ttggcaataa
     9841 aatccaatac ccggacatta tcatatcctt tcgcagcatg tttacacatt tcatacatgg
     9901 ccgttcctgt ctgccagata acttgaatgc cggcatctgc aagttcttgc aaaccgccta
     9961 tcatgcaatt attaagcgtg cgcgatccca aacttccacc aacaacaaat aatactttgc
    10021 tatcgatatc gatatcaaaa tcggcacgcg ccttttcagg tttcacattg caaagcacta
    10081 tattttgtct taccggattt cctgttttca caatttttcc tgcgggaaag aattgctcca
    10141 taccatcata ggccacgcat actgcttttg ccttcttcga cagtatttta ttggtaactc
    10201 cgggatatga attttgttcc tggattatga tcggcacttg atgacgaagt gctgcattca
    10261 gagcaggtcc acttgcaaat ccaccggttc cgatggcaat atccggttcg aattttttta
    10321 cgatgctgcc ggatttgaaa taactggaaa tcaattttat cggaaacatt aaattgcgcc
    10381 aatccaaacc tcggtgaaag ccactgatcc acaagccttc aatcggatat cctgattttg
    10441 gcaccttctc catttccatt ttatcctgcg caccaacgaa taaaatattg gcatcaggcc
    10501 tacgtcgttt tatttcgttt gcgatggcaa tggcaggaaa aatatggcct cctgttccgc
    10561 cgccacttat taatatgttg attgatgatg ccaatgcttc ttatttaata tctattttgc
    10621 taattccggt tcttcttcag attgttcttc agtttccgtt tgttcatctt cctcctcaac
    10681 ttcatcagct ttttcaattt ctctacttac acttaaaata atacctatgc taagactggt
    10741 aaaccacaaa gaggttccac ccatgctgac taatggcaat gtcaaaccgg tggtaggcag
    10801 taagtgaacg gcaactccca tatttataaa tgcctgaata cacaaacaca gtcccaatcc
    10861 tacagccagc aatgcaccaa aactagtagg tgcctttact acgattttga tacatcgata
    10921 taagaaaacc aagtacagaa atatcacaaa tatgcctccc attaatccat attcttcgag
    10981 aatggttgca aaaatgaaat cggcataagg atacggaagg aaatctttct gtgtagaatt
    11041 tcccggtcca acaccgaaca ctcctccttt ggcgatggcg attttcgcct gatctacctg
    11101 ataggtatca tcagaagttc caccgtcaac aaagctatct acacgcgctt tccaggtacc
    11161 catacgtccc acgtgcggaa atgcatatgc caatgcaatg atcaatccta tcaacacaat
    11221 acctgcgccg atggtgagta atatatattt gaatttcact ctacccacaa acatcaacac
    11281 gatgcatgta aaaaataaca cgctcgcagt agataaattt tccgggagaa ttaatgcaca
    11341 aataattgct atcggcagaa ttaccggaat aaaagcttct ttaaacgaat cgaccttatc
    11401 ttgttttttc gaaagcaccc gcgcggtata catgatgagt gcaaaacgcg caaggtcaga
    11461 tgtttgaaac gtaagattga taaccggcaa agtgatccat cgcgatgcat cgtttacttc
    11521 cggtgcaaga aacatggtgt agagtaatgc cggaacgctc agccataata atatctgggc
    11581 aattctgctg taatatttat atggtatgag gtgactgaga tacatcagca cgataccaaa
    11641 caacaagatg accgagtgtt tgaccagata atactctgta tttccaccgg ctgaacgata
    11701 tgcaagtgtt tctgttgaac tatacactgc aagaatggaa aatacagaca atacaaaaac
    11761 caccaaccag atgtaccggt cgcctctcac tctgctgaac agatattgta ttgagttaga
    11821 cataaacgct attggtgatg gttgaattaa atgaccacag gattacaaca agcgcacctg
    11881 ttttttaaat tggttgccgc gatcttcaaa attttcgaat aaatcaaaag atgcacatgc
    11941 aggcgacagt aaaacgatat cgccattttt gccaaggcga taagccattt gcacgcaatc
    12001 ttccattgtt tcggtgttta caagaatatc caccattttt ccgaatgctt cgtgaatacg
    12061 cgtattgtct tttcccagac aaacaatggc ttttactttc tttttcacga gatctgcaat
    12121 aagagaataa tcattccctt tatcgatgcc accaactatc cagatgacag ggtctgcctg
    12181 actttcaagt gcataccatg tgctgttgat attggttgct ttcgaatcgt taataaatga
    12241 aatgccgtgc acttttgcaa caaattccag tcggtgttcg agtccgctga aatctgtaaa
    12301 gctctcacgg atgctttcat ttttcaatcc cagtactttg cctacgattc ccgcggccat
    12361 gctgttgtac tggttgtgtt taccttgtag cgctaattca ttaatggtca ttgtgaagtg
    12421 gtttttgttg atttgaatat gaatggaatt atcagccacc catgcacctt catgctgtcg
    12481 gtcatccagc gtgaatgtga gtaagttggc cgggttactc agaaatggag aatgacgttt
    12541 tagcatttct tcacttgtta ccgggtcatc ggcacagtaa atgaaatgat cgccatccgt
    12601 ttgattcatg gtgattcgga attttgaatc gatatatttt tgaaattcat aattatagct
    12661 atcgagatga tcgggggtaa tattgagtaa tacactgatc atcggtttga atgtaacagc
    12721 atcatccaat tgaaaattgc ttacttccaa tacataatat tcatgattgg tatgcgcaac
    12781 caattcagca aagctcttac cgatattccc tccgattgcc acatcgcaac ctgctttttt
    12841 catcatgtgg taggtaagcg aagtacaggt agattttcca ttgcttcccg tgatagcaac
    12901 tacctttcct ttgcaataac gccatgcaaa ttcaatctcg ccaacaacaa ttttattagc
    12961 tgcgcgaatt tgcttcatca catcatattt ctcttgtatg cccggacttt taatgatctc
    13021 atcggcagca agaattatat ccatagaatg cttaccttct tcaaatgcaa taccgcgcga
    13081 agaaagttca tgcttatagc tgtcgcgtat tttccccttg tcggaaacaa atacctcaaa
    13141 cccctgctgt tgtgcaagga ttgcagcacc tgttccgctt tcaccggagc ctaatatgac
    13201 gagctttttt gccattaacg cactttaagt gttacgatgg atacaatagc taataatatg
    13261 gtgatgatcc aaaaacgtgt tacaatctta gtttcaaaca tgcccttttt ctgatagtga
    13321 tgatgaagtg gactcatcag aaaaatgcgt cttccttcac catatttttt tctggtatac
    13381 ttgaaataac tcacctgtat cattacagat aaattctcta ccaggaatat tccgcataaa
    13441 acaggaatta ataattcctt gcggatgaca attgccaatg aagcgatgat accgcctaat
    13501 gtcaaacttc ctgtatcgcc cataaatacc tgtgccggaa aggcgttata ccatagaaat
    13561 ccgatgcagg ctccgatcaa tgctgcgctg aatactacca attctccgga gtcaggtatg
    13621 tacatgatat tcagataatc actaagaatg gagttacccg cgatataggc gaaaattaat
    13681 aatgtaagta cgataatagt tgatgtgcct gccgccaatc catctagccc atcggtgaga
    13741 tttgcaccat tggatacagc agtgataatt attataatta tagcaacaaa aactatcggg
    13801 aatagccatt tctgtgtttc cggcgatgcc catgacacca accactcata atcaaattca
    13861 ttatttttta cgagtggaat tgtagacttt gttgatctgg tatcgtgact taaaaaatgc
    13921 gtgctgtcag aattcagaat accatcactt tccaatcgtg tgtcatttgc aggataggca
    13981 tctattggcc tgccgacagg ctgtccattt tcaacagcat atacatatac catgtcataa
    14041 tcacggagtt tgatgtcatc gctgaaatac aaaacaagtc caacaatagc tcccagtcca
    14101 acctgaccca tcagtttgaa ttttgcacgg aggccttctt tattttttct gaatacttta
    14161 atatagtcat ccaaaaatcc tatcaatccc atccaaacag ttacgatgag catgaggatg
    14221 acataaatat ttttcagatc ggcaagtaat aaaaccggaa taagcaatga aagaataatg
    14281 attataccac ccattgtagg tgtacctttt ttacttaact ggccttccag tccaagatcg
    14341 cgaacacttt cgccaatctg ttttttctgc agataagcga tgagtcgttt gccgataacg
    14401 atggagaaga tcaacgataa taatacggcc aaacctgcgc ggaacgagag gtattgaaac
    14461 atccccgcac cgggaacatc gtattgttga tcaagccaat taaataaata gtataacatt
    14521 agtgggccaa ggttttaaat gtttcgcgca atacttcttt atcgtcgaat ggatatttta
    14581 ctccttttat ctcctgatat ttttcatgtc ctttgccaac aacacaaatc acatcacctt
    14641 ttttcgccag cataacagca gtgcgtatcg cttcttttct atcagtgatc gacaagctgt
    14701 gtttccttgt ttcttcattc aaaccggatt ccatttcttt gatgatggca tctggttctt
    14761 cgctgcgggg attatcggag gtgagaattg ttttatcgct gatttttgcc gccgtagcag
    14821 ccatcacagg tcgttttcca cgatcgcgat cgccaccgca accaacaaca gttaagaggt
    14881 gttggtcttt ttttagcatt gatcgcacgg ttgacaataa tttttctacg gcatcaggtg
    14941 tatgtgcata atctatcaca ccggtaattg atctgtcgcc ggtataaagc acatcaaatc
    15001 ttccttcaac aggtttttga gcactcaata cggtaagcac ttgttgtttt tcaaatccga
    15061 gtaatctgaa tatggcgtat accgtaagaa tattccatgc attgaattca cctaatagca
    15121 atgagtgaaa ttccactcca tccaactgca aaacgagtcc gctgatcaca ttctctttaa
    15181 tacgagcggt aaaatcacct gtacctaaaa cgctgaaagt agcatgcttc gctttacagt
    15241 tttgcaacat gatctttccg ttgcgatcat cggagtttac aagtgcaaat gcatctgaag
    15301 tcaggccatc gaagaacatt ttcttcgcgt cgatataatc tttgaatgtt ttgtgataat
    15361 cgaggtgatc atgtgtgata ttagtaaata cacctccaac aaactgcagt cccgctatgc
    15421 gatgctgatg tgctgcatgc gaacttactt ccatgaatgc ataagcgcaa ccggcgtcaa
    15481 ccattttagc cagcagtgca tttaattgca ccggatcagg tgtggtatgt gttgaaggta
    15541 ttacctcttc gttaatttta ttttcaaccg tggaaatcaa acccgtatta aaacctaaat
    15601 cacgcattac atgatagcac aatgttgctg tggtagtttt tccatttgta cctgtgatac
    15661 caacgagttt taattttgtt gacgggtaat cataaaatgc atgcgccatt attcctaaag
    15721 cagctgcagc attgtctaca ataatcaagc atacattatc cggcacctct atttttcttt
    15781 cagcaacaat agcaacagct ccctgagcga tggcggttgg aataaaatca tatccatcta
    15841 cagtagttcc tttcacggct acaaacaaca taccttctgc agcgcggcga ctatcagctg
    15901 taataccaat aatctgaacc gacgtgaggc ttacaccgtg ttgc
//
LOCUS       DAOQHY010000070        15918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1741_length_15918_cov_5.840194, whole genome shotgun sequence.
ACCESSION   DAOQHY010000070 DAOQHY010000000
VERSION     DAOQHY010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15918
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1741_length_15918_cov_5.840194"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..1471)
                     /gene="icd"
                     /locus_tag="PLL28_07395"
     CDS             complement(170..1471)
                     /gene="icd"
                     /locus_tag="PLL28_07395"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HNF69185.1"
                     /translation="MSGQKITIQNGVLNVPDEPIIPFIEGDGTGADIWAASVRVFDAA
                     VAKAYGGKRKIHWMEVLAGEKSFNQTGNWLPDETLDAFREYLVGIKGPLTTPVGGGIR
                     SLNVALRQILDLYVCLRPVRYFEGVPSPVKSPEKVDMVIFRENTEDIYAGIDWAAGSD
                     LVGKFLNWLQENSPKDFEKIRFGNKAKTDAWEEALRSVGVPNRDVDVQVGIGIKPVSY
                     SGSERLIYSALDYAVQNNRKSLTIVHKGNIMKFTEGAFRDWGYQVARDFFGAVPLDGG
                     PWHVIPEGKPGAGIVIKDAIADITLQQVLTRPDEFDVIATLNLNGDYLSDALAAQVGG
                     IGIAPGANINYVSGHAIFEATHGTAPKYANQDKVNPGSVILSGCMMLEYMGWKEANDL
                     IIQGMEKAIAKKRVTYDFHRLMEGATMLKCSEFGDEIIANM"
     gene            1594..3048
                     /locus_tag="PLL28_07400"
     CDS             1594..3048
                     /locus_tag="PLL28_07400"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNF69186.1"
                     /translation="MNNKPQLVRSLGLSTAILLVISSMIGSGIFKKIAPMSEQLPDSN
                     LILLAWLLAGVVSMLGVFTYAGLSSMTEEGGGQFEYFRIIYGKFFGYLFGWTCFAVIQ
                     SASIASIAYVFSQSANNLLPMGDPLAAWADISIAGKIFPFSNSGVKIFTIITIAVLTL
                     INYYGVKKGGLLNDVFSAAKIAGIVFLIIAGLALHGTVTEDASINVPELPQGLSLLST
                     MFAAMLAAFWAYDGWINISYIGGEVKNPKKNIPIAIIGGTAVVMLLYTLVNYVYLHVL
                     PVSSFIAIKNSDNQIAAAEVAQHLLPGFGFTLISILIMICTFGATNASLMSSPRIYHQ
                     MAKKNLFFKSFGETHPKYHTPHISLFYQMIWSSVLVISGTFDQLTDMLIFASFIAYGS
                     GAAGLLYMKYVGKKIQMPDGTVQHQRITAKVIGFPVIPIVFLLFCIALVGNTLFTYTS
                     ESLTGLAFIAIGIPLYFVFQKSKNRSIDPIDTLE"
     gene            complement(3148..4044)
                     /locus_tag="PLL28_07405"
     CDS             complement(3148..4044)
                     /locus_tag="PLL28_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69187.1"
                     /translation="MKKYLIVFAFAPLMIACNQDKLDALEQENADLKNKSAEFKSGWD
                     AAVNEISSYMSMMNEIDSNLIEIKKTEGMIDNSLHSEGRSKEQTREEIMANIATLNDL
                     IQKNKNLVKDLDRKNKGNNLKIKELDERIALLNATLEQKEQDLAALRAELEAAKYQIT
                     NLNTDLATVTSAKQLLEEQNTQQAKVISDQTDVLNTAYYISGTFKELKELGIVDKEGG
                     FIGIGANKELTNNFDASAFTKVDIRKFKELALNSKSAKVITTHTSDSYTLVEGDKTIE
                     ELVIENPDEFWKASKFLVVLTD"
     gene            4235..5266
                     /gene="ruvB"
                     /locus_tag="PLL28_07410"
     CDS             4235..5266
                     /gene="ruvB"
                     /locus_tag="PLL28_07410"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNF69188.1"
                     /translation="MKNPNLEPTDDFMSGSDKELEKVLRPQGFYEFTGQERIIENLKV
                     FIAAAKQRGEALDHVLLHGPPGLGKTTLSFIIANELGVNMKITSGPVLEKPGDLAGLL
                     TSLEERDVLFIDEIHRLSTVVEEYLYQAMEDFKIDIMIESGPNARSIQINLNPFTLVG
                     ATTRSGMLSAPLRSRFGISSRIEYYSAQTLQQIVTRSSGILQTKITTDGAYEIARRSR
                     GTPRIANNLLKRIRDFAQVKGGGTIDVEIARFGLKALNVDEHGLDEMDNRILNDIIEK
                     FAGGPVGLTTIATAVGEDAGTIEEVYEPFLIQEGYIKRTPRGREATEKAYRHIGKVPP
                     GGAYRLFDE"
     gene            5370..6698
                     /locus_tag="PLL28_07415"
     CDS             5370..6698
                     /locus_tag="PLL28_07415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69189.1"
                     /translation="MKKLISTGLAASLLFAGTLHAQRNCDAPNSGLTPFLDLQTGTYM
                     GYQAGLYPGGTNTLTGPHLKSGKTIAKGIKPLDGDGNVNFGDGVVLVAGFGPSVPGHI
                     YNKFVEHVRTPSEKYDMNPCMDAINLCVGGKDISYPTSDSLYVAYWEGLVQNVYDVGY
                     TPQQVQIGWMYFNAKGLTEPPAFPGKSLEMEELYIQFINKAKEYFPNLKIMYMSSRHW
                     GGFADTTITEYYSLAEPASYQNSWSVKWTVEAQINGTEPRLQYKGAAPKAPYILWGPN
                     FWCDGELKRMWDDKKYICELSFDEDDGYHLSDQQDSKDALDILDVMYFGEVGKQFTKN
                     SATWEFCVPWLDTLAELHGYREDPNKTLGIFKVSPNPGVNEFYISLPTEKYAAQITIV
                     NELGQVVDAFLHEENFSTEVLIDMTGRPSGMYFAKVMMNGEVHTLRFMKQ"
     gene            complement(6963..7583)
                     /locus_tag="PLL28_07420"
     CDS             complement(6963..7583)
                     /locus_tag="PLL28_07420"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNF69190.1"
                     /translation="MRIILFTTILFTFMVADAQINAGDPKDNEFHLFDAGFSVGANFS
                     QVDGDYYAGFNKLGITAGPIIHLNFNPNWSASLELLYSQKGAKSKPDPNNVNTYLFVL
                     DYAEVPVLINYNDKNRLLFQAGLAYGRNIKAFEEVNGIDTNGGEVIKSDELSYVLGGT
                     FLIGEMKHWGANFRYEGSITKVGPSPNPKVVGLVNRLIAVRGIYYF"
     gene            7663..7935
                     /locus_tag="PLL28_07425"
     CDS             7663..7935
                     /locus_tag="PLL28_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69191.1"
                     /translation="MFKYTTNTLKKIEDLLKEAGYIVRYERGNFMAGYCILESKKVVV
                     INRYYETESRINSLLEIMGKINLEGEELSESSRDFLHEIQLQTVKV"
     gene            7932..8696
                     /locus_tag="PLL28_07430"
     CDS             7932..8696
                     /locus_tag="PLL28_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNF69192.1"
                     /translation="MIVTILGSGTSGGIPMIGCPCPVCRSTDSRDKRLRTSIHISING
                     KSFVIDAGPDFRQQMLRESISRLDAIIFTHQHRDHTAGLDDIRAYNYFQAKPMDIYLS
                     SEVEASLKKEYHYVFEGGGYPGLPEMRLFRIENRAFYIDEVKITPIEVMHYKLPVFGF
                     RVNNFTYITDANAISEQEIEKIKGSEVLIINALRKERHISHFSLEEALDMIRKINPQK
                     AYLIHMSHQMGAHAEVSMELPENVELAYDGLRIECA"
     gene            8850..11801
                     /locus_tag="PLL28_07435"
     CDS             8850..11801
                     /locus_tag="PLL28_07435"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69193.1"
                     /translation="MKKLFTLLAAFSCLLGMKAQDAHQKDPFAKTFIQNTQRVPNQQL
                     QATFRDAKAWENFRAAHGNWWVEFKEETGTPHRAYGQPIDVAGATPEDKAINFFETYC
                     KDYIPTTQGLVLTSSNTTDKYHYVSFIQQYNGLDVLWSEATVRINMQGEVAMFGLDFY
                     KGIGISTTPAIDAASIGAFATTGIDVPVLGTEVAPALKVLPIPGATGMEYKLVYEVTV
                     HTANVNDIPGAYYTLVDANNGFVYYRANRVHACGEYLMGANIQINADITDNPLLATVN
                     RGLPYLRVKVDGVNYYTDANGFLSLNTITAPTTARIYLRGLYARVSQGTGATNIDSAD
                     ITVFPGDNVIQFDDEAGTVPSEVSAYYWQNIVHDHMKSYFPSFTDLDVDQLIRVERND
                     GSCNAFYDGTSTNFYQNGGGCPSTGLFNDVVMHEYGHGVNYDLYAALGDPGGMGNGAM
                     QEGYADLWGITVTDNPVMGQGFLGGAGTFVRRYDAEPKVYPQDLVGEVHADGEIIAGA
                     WWDVSENLGGDIESMTALWMETHNATVDGPSGSEGTIYTDVLIEALFADDDNGDLTDG
                     TPNYNAIVEAFCEHGITMIGQVSTDHIGTFDFINSDEPISINVTIDFDYPTYVSDVKM
                     YYKVNNDPSWTESVMTSVDAVNYVGEIAPQIAGSVISYYIQFSDINNCGGTVLPAEAD
                     LVDPNLPYFALVGAYQWHVEDFDNVFGSWEVDPFGTDDASTGEWDINTPIPSTDDVGY
                     TVQTGEDHTVGDGNLCAFTGNAGEFDGVGTNDVDGGKTTLRSPFIDLTIYNDPIIVYY
                     RKYSNDSPTSANPGNDVWQVYITDNGGDSWMKLERTYTSDNTWRRNAFRVKDYVDVTE
                     DFGMIFIAQDSIDPSDPSGFLGGSLVEAAIDDMEIWDTRMPEVHVNDIETNSITVYPQ
                     PTNSYLNIVSDILISQVCLIDISGKIVSAESFDNLHLVSLEVGQIPKGIYSLKLNFCN
                     STTGYKTVIIN"
     gene            complement(12508..14199)
                     /locus_tag="PLL28_07440"
     CDS             complement(12508..14199)
                     /locus_tag="PLL28_07440"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNF69194.1"
                     /translation="MKKLLLSTFVLISLLTSVQTAFSQATEKYYLRRTPPEPWTWAPI
                     LNTNITEMDNVFGIGGWNSGYYSTVDPDVVFSDNTCFVFMEGGDDHAIDMKNFIDANI
                     TLIESWVTNGGNLFLNAAPNEGVDINCGFGGIVINYPDYASNVNAVDPTHPIFVGPHV
                     PVGTSWSGTYFGHATILGPGLTTLIEDTPSGKAVCSELSWGGGKVIFGGMTVTSWDSP
                     SPQCINVRMNIFEYLSSYVFLDFTYPDSIYCQFEDDPLPVFAVGADTGIFVATPTGLV
                     IDSITGEIDLSASLPGVYEVSNFMDGLSCASGSSTFTVTIAGTPVADAGPDVSLCRGD
                     SVQLDGSGGVTYLWTPPVYLDDPTLEDPTVQAPPTNMFYQLIAYNEFGCPDTDDVMVT
                     LYPDPVIDAGEDQVMVLGGFAALDASGGATYVWTPAEWLDNALISNPTAFPEDTTIFV
                     VIGTDINGCVDTDSVTIFVVEESDILSPTAFTPNGDGLNDTYKPSFVGIGSITEFSIY
                     NRWGSLLYYTADPTLGWDGNFKDNEQEVGTYVVVIKGLTQQGDPIVKTSTLALLR"
     gene            complement(14377..>15918)
                     /locus_tag="PLL28_07445"
     CDS             complement(14377..>15918)
                     /locus_tag="PLL28_07445"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type H(+)-translocating pyrophosphatase"
                     /protein_id="HNF69195.1"
                     /translation="GGIYTKAADVGADLVGKVEAGIPEDDPRNPATIADNVGDNVGDV
                     AGMGADLFGSYVATVLATMVLGREIQSDDTVGGMAPMLFPMLVAAIGLLASIVGTWFV
                     RVKEDGNVQRALNMGNWIAIAITAVAAWFLAGWILPAQMTMIKTGEILTPGGVYASIV
                     TGLVVGTLISFFTEYYTSMGKRPVTAIVEQSSTGHATNIIGGIYIGMQSTMLPTIFLA
                     VGIVLAFIFAGIYGVAIAAAGMMATTAMQLAIDAFGPIADNAGGIAEMSQLPKEVRHR
                     TDILDAVGNTTAATGKGFAIASAALTSLALFAAFMGTAGLQTIDISKAPVLAGLFIGG
                     MIPFLFSSMAIKAVGKAAKSMVEEVRRQFREIPGIMEGTGKPDYDRCVAISTEAAIRQ
                     MILPGALALLTPVLVGFGLKGVFDNATSAEILGGLLAGVTVSGVLLAIFQSNAGGAWD
                     NAKKSFEKGVEIDGETHFKGSDAHKASVTGDTVGDPFKDTSGPSMNILIKLMSIVSLI
                     IAAYL"
BASE COUNT         4701 a         3386 c         3245 g         4586 t
ORIGIN      
        1 ttaccaggct cacaacaatc atggtgaggt cgaatctttt gagttggggt tgaatggaca
       61 tttgtcaaat gtaaagaact gtaaccatat taaaaacaaa gagcctcccg aaggaagctc
      121 tttgttttta gattgtaatg attatcctaa actattggaa ctatttcaat cacatattcg
      181 caataatctc atcgccgaat tcggagcatt tcaacatcgt tgctccttcc atcaggcgat
      241 ggaaatcgta agtaacacgt tttttggcga tggctttttc cattccttga atgataagat
      301 catttgcttc tttccatccc atgtattcaa gcatcataca tccacttaaa attactgatc
      361 ccggatttac tttatcctga ttagcatatt ttggtgctgt accatgcgta gcttcaaaaa
      421 tggcgtgtcc gcttacatag ttaatgttcg cacctggagc aataccgata ccaccaactt
      481 gagcggcgag tgcatcgctg agataatcgc cattgaggtt taatgtggct attacatcaa
      541 actcatcagg acgagtaagt acttgttgca aagtaatatc tgcaatagca tctttaatta
      601 caatacctgc accgggtttt ccttcaggga ttacatgcca tggaccacca tccagtggaa
      661 cagcgccaaa gaaatcgcgt gcaacctgat aaccccaatc gcggaaggca ccctccgtga
      721 atttcatgat attgcctttg tgcacgatag taaggctctt gcgattgttt tgaacagcat
      781 aatcaagagc agaataaatc aaacgctctg aacctgaata gctcacaggt ttaataccaa
      841 tgcccacctg aacatcaacg tcgcggtttg gaacacctac ggagcggagt gcctcttccc
      901 aggcatcagt cttggcttta tttccaaaac gaatcttttc gaagtcttta ggtgaatttt
      961 cttgaagcca attgaggaat ttacctacca aatcacttcc tgctgcccaa tcgataccgg
     1021 cataaatatc ttcagtattc tctctgaaaa taaccatgtc aactttttca gggctcttca
     1081 caggtgaagg cacaccttcg aaataacgaa caggacgaag acatacatac aagtccagga
     1141 tctgacgcag tgcaacgttc aatgagcgga taccaccacc taccggcgta gtcaagggtc
     1201 cttttatgcc taccagatat tcacggaagg catccaatgt ttcatcagga agccagttac
     1261 cggtttggtt aaaagatttc tcacctgcaa gcacttccat ccaatgaatc ttgcgcttgc
     1321 caccatatgc tttggcaaca gctgcatcga aaacgcgcac tgaagcagcc cagatatcgg
     1381 caccggtgcc atcgccttca ataaatggaa taatgggttc atcgggcaca ttgagcaccc
     1441 cgttttgtat cgtaattttt tgtccgctca tgataatatg ttatttgaaa tgttgcgcaa
     1501 agatacgatt ctgcgggcag agctgacagg aatatcctca ggttgttcac tttgtcggtg
     1561 gtccgtttgg tgttaattcc ctaatcttac ggtatgaata acaaaccgca gcttgtaaga
     1621 tcactgggac tgagcacagc cattttgctt gttatcagct ctatgatagg cagcggaatt
     1681 tttaaaaaga ttgctccaat gagtgagcaa ctgcccgaca gcaaccttat tttgcttgcc
     1741 tggttgcttg ccggtgtcgt ctcgatgctg ggtgtattca cctatgccgg attgagctcc
     1801 atgacagaag agggtggggg gcagtttgaa tattttcgaa taatttatgg gaaatttttt
     1861 ggttatctgt tcggatggac atgctttgct gttatacaat ccgcatccat tgcttctatt
     1921 gcctacgtat ttagtcaatc ggcaaataat ttattgccaa tgggcgatcc gcttgccgcc
     1981 tgggccgata tatcaatagc cggaaagatt tttccattta gtaacagcgg agtgaaaata
     2041 tttaccatca ttacaattgc tgtgcttacc ttgatcaatt attatggagt aaaaaaaggc
     2101 ggactattaa atgatgtatt ttcagctgca aaaattgccg gtattgtatt tttgattatt
     2161 gccggacttg cattgcatgg cactgttact gaagatgcat ccattaatgt tccggagctg
     2221 cctcagggat tatcgctttt gtcgaccatg ttcgcagcca tgcttgcggc attttgggct
     2281 tatgatggat ggataaatat ctcttacatc ggtggcgaag taaaaaatcc caaaaaaaat
     2341 attcctattg ccatcatagg tggaacagca gtagtgatgt tgttgtatac cttggtgaat
     2401 tatgtatatc tgcatgtatt accggtatcg tcatttattg cgataaaaaa ttcagataat
     2461 caaatagcag ccgcagaagt tgcacagcat ttacttccgg gttttggatt tacgttgatc
     2521 tcaatattaa ttatgatttg caccttcgga gcaacaaatg caagtctgat gtcatcgcca
     2581 cgtatctatc atcaaatggc gaagaaaaat ttatttttca aaagcttcgg cgaaacacat
     2641 ccaaaatacc atacaccgca tatttccctt ttttatcaaa tgatatggag cagcgtatta
     2701 gttatatcgg gcacttttga tcagcttacc gatatgctca tatttgcttc atttattgcc
     2761 tatggaagtg gcgcagcagg cttgttgtat atgaagtatg ttggcaaaaa aattcaaatg
     2821 cctgatggta ctgtgcagca tcagcgcata acggctaagg tgattggatt tcctgtcatt
     2881 ccaattgtat ttctgctttt ctgtattgcg ctggtgggaa acactttatt tacttataca
     2941 tcggagagcc tcacagggct ggcattcatc gccattggta taccactgta ttttgttttc
     3001 caaaaaagta aaaaccggtc aatagaccca atcgatacgt tggaataaca atttacatta
     3061 caattttaaa ttgcataaat aaagaaaggg gcaccgaggt accccttttc tttagatgtt
     3121 aacttccatt acagccatat ggaagtgtta atctgtcagc acaaccagaa attttgaagc
     3181 tttccagaat tcatccggat tctcaattac gagttcttca atagttttat cgccttcaac
     3241 aagtgtatat gaatctgaag tgtgtgtagt aatcactttc gctgatttag aattcaatgc
     3301 taattctttg aatttgcgaa tgtccacttt ggtgaatgca cttgcatcaa agttattagt
     3361 cagttctttg ttagcaccta ttcctataaa gccaccttct ttgtcaacaa taccgagttc
     3421 ttttaattct ttaaaagttc cactgatata gtatgcagtg tttaaaacat cagtttgatc
     3481 tgaaatcact ttcgcttgtt gcgtattttg ttcttcaagc aattgttttg cagaagtaac
     3541 agttgcaagg tcagtattca aattagtgat ttggtatttc gctgcctcta attcagcacg
     3601 caaagcggca agatcctgct ctttttgctc gagtgttgca tttaacagtg caatgcgctc
     3661 gtcaagttct ttaatcttga gattattgcc tttgtttttg cgatcgaggt cttttacaag
     3721 atttttgttt ttctgaatca gatcattcaa tgtagcaata tttgccatga tctcttcacg
     3781 agtttgttct ttagagcgac cttcagaatg caaactatta tctatcatgc cttctgtttt
     3841 cttgatctct atcaagttcg agtcgatctc gttcatcatg ctcatgtaag aactaatctc
     3901 attcactgca gcatcccatc cggatttaaa ttcggcactc ttatttttta gatctgcatt
     3961 ttcctgctcc agcgcatcca acttatcctg gttacatgcg atcatcaaag gagcgaacgc
     4021 gaatactatc aaatactttt tcatcgtgtt gtgatttggt tgatatatag aaaatatttt
     4081 gccaagttat tcaaaatcgt caaaaccctt gaaaaacaag ggttttcatt tattaacgac
     4141 aaaattaatt ccttttatat tgaggaattg ctccccaata tgtggaaata aggcagtatg
     4201 ttttcaataa cctacacctt atctttgaac ttccgtgaag aatcccaacc tcgaacctac
     4261 cgatgatttt atgagcggtt ccgataagga actcgaaaaa gtattgcgcc ctcagggatt
     4321 ttatgaattt acaggtcagg agcgaatcat cgaaaacctc aaagtattta ttgccgccgc
     4381 aaagcaacgc ggggaggcat tagaccatgt gctgcttcac ggccctcctg gattaggaaa
     4441 aacaacgctt tcttttatca tagcaaatga attgggtgta aatatgaaga tcaccagtgg
     4501 tccggttttg gaaaaacccg gcgatctggc aggattactt accagtctgg aagaacgcga
     4561 tgtactgttt attgatgaaa tacacaggct gtcaacggta gttgaggaat acctctatca
     4621 ggcgatggag gattttaaga ttgatattat gattgaatcc ggcccgaatg ccaggtcgat
     4681 acaaatcaat ttgaatccat tcacgcttgt aggcgctacc acaagatccg gcatgcttag
     4741 cgctcctttg cgttcaagat tcggtatctc ttcacgcata gaatactatt cggcacaaac
     4801 actgcaacaa atagttacac gctcatcagg catactccaa accaagatta ccaccgatgg
     4861 agcctatgaa atagcccgca gaagtcgcgg tacccctagg atagccaata acctccttaa
     4921 acgtatccgt gacttcgccc aggtaaaagg cggtggaacc atagatgttg aaatagctcg
     4981 gttcggactc aaagccctta atgtggatga acatggcctc gacgaaatgg ataaccgtat
     5041 cctgaacgat attattgaga aatttgccgg tggaccggtt ggccttacca ccattgctac
     5101 tgccgttgga gaagatgccg gcaccattga agaggtgtat gaaccctttt tgattcaaga
     5161 aggatacatt aaaagaactc ctcggggaag ggaggcaaca gaaaaggcct atagacacat
     5221 aggtaaggtt cctccgggtg gcgcctacag attgttcgac gaatagccaa ccttttacaa
     5281 gtaagtatcg tcaacattac atccggtttt aacacccgac atttgttatc tttgcacatc
     5341 aatttaacgg caacttaaac tattaagcct tgaaaaaatt aatctccacc ggtttggcag
     5401 cctccctgct gttcgccgga acattacacg cacagcgcaa ttgcgatgcc cctaactcgg
     5461 ggttgactcc ctttcttgat ttacaaacag gcacctacat gggctaccag gcaggattat
     5521 atccgggtgg taccaatacc cttacagggc ctcacctgaa aagtggcaaa acaatcgcca
     5581 aaggcataaa accacttgat ggcgacggca atgttaactt cggagacgga gtagttttgg
     5641 ttgcaggttt cggcccatca gttccgggac atatttacaa caaatttgta gagcacgttc
     5701 gtactccttc tgaaaagtat gatatgaatc cttgtatgga tgctatcaac ctttgtgttg
     5761 gaggaaaaga tatcagctac ccgacatcag atagccttta tgttgcttat tgggaaggac
     5821 tcgtacaaaa cgtttatgat gttggatata caccacaaca agtacagata ggctggatgt
     5881 atttcaatgc gaaaggtctt acagaaccac cggcatttcc gggcaaatct ttggaaatgg
     5941 aagaacttta tattcagttc atcaataagg caaaagaata ttttccaaac ctgaagatca
     6001 tgtatatgag ttcacgccac tggggtggat ttgctgatac tactatcact gaatattatt
     6061 cacttgcgga gccggcatcg tatcaaaaca gctggtcggt aaaatggact gttgaagcac
     6121 aaatcaatgg aacagaacca cgccttcaat ataaaggcgc agcgccaaaa gcaccataca
     6181 ttttatgggg accaaacttc tggtgcgatg gcgaattgaa gcgcatgtgg gatgataaaa
     6241 aatacatctg cgaattatca ttcgacgagg atgatggata tcacctcagc gatcaacaag
     6301 actccaaaga tgcattggat atcctcgatg tgatgtattt tggcgaagtt ggaaaacaat
     6361 tcacaaaaaa tagtgcaaca tgggaattct gcgtgccttg gttagatact ctggctgaat
     6421 tacatggtta ccgcgaagac ccgaataaaa cactgggtat tttcaaagta tctcctaacc
     6481 ccggagtaaa tgaattttat atcagtctgc ctacagaaaa atatgccgct cagatcacca
     6541 tcgtgaacga actcggacaa gtagttgatg ccttcctcca cgaagagaat ttctcaaccg
     6601 aagtgttgat tgatatgaca ggacgccctt caggtatgta tttcgccaaa gtaatgatga
     6661 atggtgaagt gcatacattg cgatttatga agcaataatt caaaatccct tatcatatca
     6721 aaacgccgca tggatatttt ccatgcggcg ttttgctttt catctcaatt tgaatctgcg
     6781 attgtttgga ttactcgtat tagaatctag aattggtatc aagtatcagg tatcaggtat
     6841 caggtatcaa gtatcaggta tcaagtatca ggtatcaggt atcaggtatc aagtattatt
     6901 caccactcac tactcaccac tcaccattca gcactcacca ctcaccactc accactcacg
     6961 tcctagaaat aataaatccc cctcaccgct atcaacctat tcaccaaacc aaccaccttt
     7021 ggattgggtg atggacctac tttggtaata gatccttcat atctgaaatt agcaccccag
     7081 tgtttcattt caccaattaa aaaggtgcca ccgagaacat aactcaattc atcagattta
     7141 atgacttcgc ccccatttgt atcaatgcca ttcacctctt caaaggcctt gatatttcgt
     7201 ccataggcta agcctgcctg aaataacaat ctgttcttat cgttataatt aatcaataca
     7261 ggaacttctg cataatcgag aacgaatagg taagtattaa cgttatttgg atcaggtttt
     7321 gacttagcac ctttttgtga ataaagcagt tcgaggctcg cactccaatt aggattgaaa
     7381 ttgaggtgaa tgattggtcc ggctgtaata ccaagtttat tgaagccggc ataatagtcg
     7441 ccgtcgactt gtgaaaaatt agctccgaca ctaaaaccgg cgtcgaacaa gtggaactca
     7501 ttgtccttcg ggtcaccggc attgatctgt gcatcggcaa ccatgaatgt gaataggatg
     7561 gtggtaaaaa gaattatcct catttgatgt gcattacttc gttttcaata cagcggcact
     7621 gccattattt ttggcagcac aaagtaaata acgacattcg tgatgttcaa atatacaacc
     7681 aacaccctaa aaaagatcga agacctgtta aaggaagccg gatacatcgt tcgctacgaa
     7741 cgcggcaact ttatggccgg ctattgtatc ctcgaaagca agaaagtggt ggtaatcaac
     7801 aggtattatg agaccgaatc ccgcatcaac agcctgctag agatcatggg taagatcaat
     7861 ctggaagggg aggagctcag cgaatcgtcg cgcgattttc ttcatgagat acagctgcaa
     7921 acagtaaaag tgtgatcgta accatactcg gctcgggaac ttccggtggt ataccgatga
     7981 taggatgccc ttgtccggta tgtcgcagca ccgattctag agataaacgc cttcgcactt
     8041 ccatccatat cagtatcaac ggaaaatcgt tcgtcatcga cgcaggtccc gatttccgcc
     8101 agcaaatgct ccgggagtcc atatcgcgcc tcgatgcaat catttttacc catcagcacc
     8161 gcgatcatac cgcaggactc gatgatatcc gcgcctataa ttacttccag gcaaaaccaa
     8221 tggatatcta cctcagcagt gaggtggagg cttcgttgaa aaaagaatat cactatgttt
     8281 ttgagggagg cggatacccg ggtttacctg aaatgcgcct tttcaggatt gaaaatcgag
     8341 ctttttacat cgacgaagtc aaaatcacac ccatagaggt catgcattac aaattgccgg
     8401 tttttggctt tcgggtgaat aatttcactt atatcactga cgcaaatgcc atttccgagc
     8461 aggaaattga gaaaattaaa ggctctgaag tattgattat caatgcttta cgaaaagaaa
     8521 gacatatttc ccatttctcc ttggaagaag ccttggacat gatccgtaag ataaatccac
     8581 aaaaagcata cctcattcac atgagccatc aaatgggcgc gcatgccgaa gtaagcatgg
     8641 aactgccgga aaacgttgaa cttgcttacg acggcctgag aattgaatgt gcataacaga
     8701 ttgccacctg cgcttatgac cttactctga caaatgcccg gttgtgtagc taaaaacgct
     8761 ttattatctt tgatatccgt ttgatcctgg aacaaccaca actcaatcag gcattaacat
     8821 caaacagtaa tcaaatcttc acaccaatta tgaaaaaatt gtttacgctc ctcgcagcat
     8881 tttcttgctt gttggggatg aaggcacagg atgctcatca aaaagatcct ttcgccaaaa
     8941 cattcatcca aaacacgcaa agagttccaa atcagcaact acaagccact ttccgcgatg
     9001 ctaaagcatg ggaaaatttc cgtgccgcgc atggcaattg gtgggttgaa ttcaaagaag
     9061 aaaccggtac tccacaccgt gcttatggac aaccaattga tgttgcaggt gcaactcctg
     9121 aagacaaggc aattaatttc ttcgagactt attgcaaaga ttacatccct actactcagg
     9181 gtttagtact tacttcaagc aacacaactg acaagtacca ctatgtaagc ttcattcaac
     9241 aatacaacgg acttgatgta ttgtggtctg aagcaaccgt gcgtatcaat atgcaaggtg
     9301 aggttgctat gtttggtctt gatttttata aaggcattgg catctctaca actcctgcaa
     9361 ttgatgcagc gagtattggt gcatttgcta caacaggtat cgatgttcct gttcttggaa
     9421 cagaagtagc acctgcattg aaggtgttac caattcccgg tgctacaggc atggaataca
     9481 aacttgttta cgaagtaaca gtgcatactg caaacgtaaa tgacattccc ggagcatatt
     9541 acacattggt agatgcaaat aacggtttcg tttactatcg tgcgaatcgt gtacatgcat
     9601 gcggtgaata tttaatgggt gctaatattc aaatcaatgc tgatatcact gataatccac
     9661 tgttagctac agttaaccgc ggattgccat accttagagt aaaggttgat ggtgttaatt
     9721 attatacgga tgccaacggg tttctctcac taaacacaat tacagcacct actactgcta
     9781 gaatttattt gcgtggttta tatgctcggg tcagtcaagg aaccggtgca actaatattg
     9841 atagtgccga tatcactgtg tttccgggcg ataatgttat tcaatttgat gatgaagccg
     9901 ggacagtgcc atcggaagtt tccgcatact actggcaaaa tattgtgcac gatcatatga
     9961 agtcttattt cccatcattt actgatcttg atgttgatca gttgatacgt gtcgaacgca
    10021 acgatggatc ctgtaatgcc ttttatgatg gaacatcaac taacttctat caaaatggtg
    10081 gcggctgccc ttcaacaggt ttatttaatg acgttgtgat gcacgaatat ggccatggcg
    10141 ttaattatga tttatatgct gcacttggcg acccgggtgg tatgggcaac ggcgctatgc
    10201 aggaaggtta tgccgatttg tggggaataa ctgttacgga taaccctgta atgggacaag
    10261 gtttcctcgg tggtgcagga acatttgttc gtcgttatga tgctgaaccc aaagtttatc
    10321 cgcaagacct cgtgggcgag gtgcatgccg atggcgaaat tattgcaggt gcgtggtggg
    10381 atgtttctga aaacttagga ggggatatag aatcaatgac tgcactatgg atggaaacac
    10441 ataatgctac agtagatggg ccaagtggtt ctgaaggtac aatatataca gatgttttga
    10501 ttgaggcatt atttgcagat gatgataacg gtgatttgac agatggaacc cctaattata
    10561 atgcaatagt tgaagcgttt tgtgagcatg gaataacgat gattggacaa gtttcgacag
    10621 accatattgg aacatttgat ttcataaatt cagatgaacc aatttcaata aatgttacca
    10681 ttgattttga ttatccaacc tatgtcagcg atgtcaagat gtattataaa gtcaacaatg
    10741 atccttcttg gactgaatct gttatgactt cggttgatgc tgtaaattac gttggcgaga
    10801 ttgcacctca aatagcaggt tcagttattt catattacat tcaattttct gatattaata
    10861 attgtggtgg tacagttttg cctgcagaag cagatttggt tgatcctaat ttaccttatt
    10921 ttgcacttgt gggcgcatat caatggcatg tagaagattt tgataatgtt tttggaagct
    10981 gggaagtaga cccatttgga acagatgatg ctagcacagg tgaatgggat ataaataccc
    11041 caattccttc taccgacgat gtgggttata cagttcaaac aggtgaagat catacggtgg
    11101 gtgacggaaa cctttgtgct tttactggca atgcaggtga gtttgatggt gtgggtacaa
    11161 atgatgtaga tggcggcaag acaacattga gaagcccatt tattgatttg actatttaca
    11221 atgacccaat tatagtttac tatcgaaaat attctaatga ttcgcctaca agtgccaatc
    11281 ccggtaatga tgtttggcaa gtatatataa ctgataacgg aggtgattca tggatgaaac
    11341 ttgagagaac ttatacttca gataatacgt ggagaagaaa tgcttttaga gtgaaggatt
    11401 atgtagatgt aactgaagat tttggaatga ttttcattgc acaggacagt atagatccat
    11461 cagatcctag tggcttttta ggcggaagct tagtggaagc tgcaattgat gacatggaaa
    11521 tctgggacac acgaatgcca gaagtacatg taaatgatat cgaaacaaac tcaatcactg
    11581 tttatccaca accgacaaat tcatatttga acatagtttc ggacattctc atttctcagg
    11641 tttgtttaat tgatattagt ggcaaaattg tttctgctga aagttttgat aatttacatc
    11701 tcgttagtct agaagtggga caaattccta aggggattta ctcattaaaa ttgaattttt
    11761 gtaactctac gactggttac aaaacagtaa ttataaatta atttcaataa taaaaacgac
    11821 aggcaagacc tgtcgttttt attatttgtt gaaaaccgaa attgagaaca ataaatttaa
    11881 ggattttgtc cctatttttg aattgttatt agtcttgaag attgaaattg ttcaccagtt
    11941 aaatttatta aatactcacc actagataga ttcgataaat taaaatttat ttgagtattt
    12001 tctagttcaa tgtttgtgaa actttctaca agaattccgg atattgagta tacatcaata
    12061 ttgactgaac taatcgaatc atcatacaac actgatgatc taataaaata attacaaaac
    12121 gccgttcaga ttctggacgg cgtttttgtc tgatgtctct aattcagaaa tacttttctg
    12181 tttctataaa agctaatcga gttatattag taattgtatg tagctaagaa ttaagttaag
    12241 ttgaatatca ataatggatt tttataatgg aattaatgaa tagattttac caatcatagt
    12301 catcctcaac ttcaaattga atcccttttt tcgcgcaaag tcgcaaaggg gcaaagtcgc
    12361 aaagcctcat ccgccaaagg attggcaagc tcacaacgta cgactcacga ttcacgcttc
    12421 acgactcacg agtcacgcct cacgcctcac gaaaaaagcc gatatcaaat cgacatcggc
    12481 tttttagaat atgataaaca ttgatcgcta tctcaacagc gccaacgtac tcgtcttaac
    12541 tatcggatca ccttgctgcg tcagaccttt aatcactact acataagtac caacttcttg
    12601 ttcattatcc ttgaaattgc catcccaacc taatgtagga tcagcagtat aatacaacaa
    12661 gcttccccag cgattgtaga tactgaattc tgtaatagat ccgataccaa caaaagatgg
    12721 cttgtaggta tcatttaatc catcgccgtt aggtgtgaat gcagttggtg aaagaatatc
    12781 tgattcttca accacaaaaa tggtaactga atcggtatca acacaaccat taatatcagt
    12841 tccgataact acaaaaattg ttgtatcctc agggaatgcg gttggattag atatcaatgc
    12901 attgtctaac cattctgcag gcgtccaaac atatgttgca ccacctgacg catccaatgc
    12961 tgcaaatcca cctaacacca ttacctgatc ttcacccgca tcaataaccg gatccggata
    13021 caacgttacc atcacatcat ctgtgtcagg gcaaccgaat tcattatagg caatgagttg
    13081 ataaaacata ttggtaggtg gtgcctgcac cgttggatct tccagggtcg gatcatcgag
    13141 gtaaaccgga ggtgtccaca aataagtaac gccaccgctg ccatctaatt gaaccgagtc
    13201 gccacgacat aatgaaacat caggtcctgc atcagcaact ggcgttcctg ctatggttac
    13261 cgtaaatgta gatgaaccac tcgcacaaga taaaccatcc atgaagtttg atacttcata
    13321 aactccgggt aatgaagcac tcagatctat ttcacctgta atgctgtcaa tcacaagtcc
    13381 ggttggcgtg gcaacaaaaa ttcctgtatc agcaccaact gcgaaaaccg gcagtggatc
    13441 atcctcaaac tgacaataga tggaatcagg gtatgtaaaa tcaaggaaaa cgtatgagct
    13501 gagatattca aaaatattca tgcgcacatt aatacattgc ggtgatggtg aatcccatga
    13561 tgtaacagtc ataccaccga aaataacctt accaccgccc caagataatt cagaacaaac
    13621 ggctttacct gaaggtgtat cttctatcaa tgttgttaat cccggaccta aaatggtagc
    13681 gtgaccaaaa tacgttcctg accaactagt tccaacaggt acgtgaggac ctacaaatat
    13741 cgggtgcgtg gggtcaacgg catttacgtt actggcgtaa tcgggataat taattacaat
    13801 gcctccaaag ccgcagttaa tatctacacc ttcgttaggt gccgcattca gaaacaggtt
    13861 tccgccatta gtaacccagc tttcaatcag cgttatgttg gcatcaataa aattcttcat
    13921 atcaatggcg tggtcatcgc caccttccat aaatacaaag caggtattgt cagagaacac
    13981 cacatctgga tccactgtag agtagtagcc cgaattccac ccaccaatac caaacacgtt
    14041 gtccatttcg gtaatgttgg tattcaggat tggtgcccag gtccacggtt cagggggagt
    14101 tctgcgcaga taatattttt ccgtagcctg actaaaagct gtttgaacag aagtcagcag
    14161 gctaatcaaa acgaatgttg agaggagtag ttttttcata gttgccaatt gcatcggtat
    14221 gttataacat agtattccac tctaaagtta atgtgaattt cggttttttg gcactttgaa
    14281 tgttaaaaac gctttaattc ataaaaaaat gagccttttc agacgaaaaa aaggcatcca
    14341 cattgggatg cctttcaaaa tatgattcga ctgtaattat aaatatgcgg ctatgatcag
    14401 gctgacaata ctcatcagtt tgatgagaat gttcatactt ggacctgaag tatctttaaa
    14461 tggatctcca actgtatcgc ctgtaacaga cgccttgtgt gcatcagaac ctttaaagtg
    14521 tgtctctccg tcaatttcaa cacctttttc aaatgatttc tttgcattat cccatgcgcc
    14581 accggcatta ctttggaaga ttgccaatag cacaccgctc acggttacac ctgcaagcaa
    14641 accaccaaga atttcagcgg aggtggcatt gtcaaataca cctttaagcc cgaaacctac
    14701 gagcaccggt gtcaataatg ctaatgcacc cggtaaaatc atctggcgaa tagcagcctc
    14761 agtagaaatg gccacacaac gatcgtaatc aggcttgcct gttccttcca tgattcccgg
    14821 aatttcgcgg aactgacgac gcacctcttc aaccatgctt tttgcagctt tacccacggc
    14881 tttgatggcc atagagctga ataagaatgg aatcatgcca cctataaaga gacctgccaa
    14941 cacaggtgct tttgaaatat ctattgtttg taagcctgca gttcccatga atgcggcaaa
    15001 cagtgctaat gaagtaagtg ctgctgatgc gatggcaaag ccttttcctg tagctgcggt
    15061 ggtatttcca acggcatcga ggatatcggt gcggtggcga acttcttttg gtagctgact
    15121 catttcggca ataccacctg cattatcggc aataggtccg aaggcatcaa tggccaactg
    15181 cattgctgtt gttgccatca tacctgcagc tgcaatcgcc acaccataaa ttccggcaaa
    15241 tataaaagca agtacaatac ctactgcgag gaaaatggta ggcaacatag tagattgcat
    15301 accgatatag attccaccaa taatgtttgt agcgtgccct gtactgctct gttcaacgat
    15361 ggcggttaca ggacgtttac ccattgatgt ataatattct gtgaaaaagc tgatgagcgt
    15421 tcctaccacc aaaccggtta cgatacttgc gtaaacaccg ccaggggtaa ggatttcacc
    15481 tgttttgatc atggtcattt gggcaggaag tatccagccg gcaagaaacc atgcggcaac
    15541 agctgtaatg gcaatggcga tccagttacc catgttcaga gcgcgctgaa cattgccatc
    15601 ttctttaact cttacaaacc aggttccgac tatagatgcc agcaaaccga tagcagcgac
    15661 cagcataggg aataacatcg gagccatgcc accaacggta tcatcagatt gaatttcacg
    15721 acctaacacc atagttgcca atacagttgc cacataactt ccaaacaagt cggcacccat
    15781 acctgccacg tcgcctacgt tatcacctac gttatcggcg atagttgcag ggttgcgtgg
    15841 gtcatcttca ggaattcctg cttccacttt tccaaccaag tcggcaccaa cgtctgcggc
    15901 ttttgtatat ataccacc
//
LOCUS       DAOQHY010000071        15906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1742_length_15906_cov_6.647909, whole genome shotgun sequence.
ACCESSION   DAOQHY010000071 DAOQHY010000000
VERSION     DAOQHY010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15906
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1742_length_15906_cov_6.647909"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1450
                     /locus_tag="PLL28_07450"
     CDS             <1..1450
                     /locus_tag="PLL28_07450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69196.1"
                     /translation="SFDQDRNFLIMKISAGGTPVWTRSYNGTSTKQSTDDALSVAVDA
                     SNNIYAAGFLKNSGTGYDLLVMKLSTAGDSLWSYIYNDAASNETDKALSIQMSPSGNL
                     VVCGRSDNDLTAVSDDDILVITLNTSGGVVWTARYQGTGGGDDIPRQMVVAPSGNIYV
                     TGKTTSATDIDALLIKYNTSGVQQWVRKTDIGVEDEFTAIQIDGEENVFVAGNTITAT
                     GNFDVITASFDASGNELFTTTYDHGENGNDEAKGLAIFDDGSFAVSCTLAPDTDAITL
                     NGDIAAVYFDTNGNWQSTTLFDGGANDDASESAYASNTGFVICGQTDNGEAGNQDYDY
                     ITIYLDETGTITNTELYNGAAGGNDVANTLMLYDNGTYISGGSEGGQRDIVTILYGSN
                     PVSISNNVASKNVQVYPNPTVATCTVYLDEINLSSSATLKLYGGNGNCVMQKAISSND
                     NIQLTLSTMPAGMYLLVITDGDQVYSTNLIKQ"
     gene            1477..2526
                     /locus_tag="PLL28_07455"
     CDS             1477..2526
                     /locus_tag="PLL28_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69197.1"
                     /translation="MQARIKKSIICKEITSYQIDSTRNNTYTPIDGDVALFEVLEIGK
                     HQSIQSETKRIVSIFEGDVIMAAFANRYATSQFEGYVPDRPTEILDILGAGGAIGLVA
                     SKNAALEDIEPTKLRLIGFATTTEGQIINTKFLHTARHTFNGYVPNNAKIILSVGSSM
                     DSGKTTSAAFTARGLKTTGKKVAFIKLTGTSFTKDKDFVFDCGADVTVDFTDCGYPST
                     YMCSKEEILDVYQSLLMQLEPHAPDYIVMEIADGLLERETDFLLRDKAFMQTIHSMIF
                     SCGDSLGVFYGLQLLHSFGIVPAVICGRFTMSPLLIQEVKAKTNIPTLDIDQLANGEY
                     IHVFDKSGMQIVHAA"
     gene            2532..4199
                     /locus_tag="PLL28_07460"
     CDS             2532..4199
                     /locus_tag="PLL28_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF69198.1"
                     /translation="MNIIKTYYSRHKGLLISVIGLGLFSNALKLAIPLCVGKFYQLAL
                     DGGSARSGIFDALFGNVQSINIFFSIFFGILLLRFIVQFAEQYLIGMTGETFSAWLRE
                     TLFTKQLHTVYSVFTQKDPGKYLLRYSGDMSALRLFITKGQIGAITDFAFLIMTIGLF
                     GWLNITLTYLLLIAIPAIFLISILINRFMRPSTEKRRDINATNLNFVSERLHAMATIK
                     VMNRSTPEINKFQRRSTKLLEAGKVYQFWNALNKALYPFLLYLLLAFVMLMIYKLLSN
                     NTITADGGTLLIFVMLIIQIIPVVKRLLRSGAIRESGKLSLKKCKLLLEAPEDSREGH
                     AMPVKKGDIRINNLSIKFGGKTIFENASFHFPPKGITLISGTGGSGKSTLLKYISGLT
                     IPESGEIMIDDASTKSYSIFQVRKKITFISQELPLIGKHVYDAISYNRDPDKKHDAMQ
                     ILSAIGFCDKFQDEVLELPLEKGGANFSTSQQQLLRIARGLITGKKIILLDEPFEGLD
                     DRSARQVAYHLTHISSSHTIILVSKSIPETLFIQHRISLDKLKQPNV"
     gene            4192..4596
                     /locus_tag="PLL28_07465"
     CDS             4192..4596
                     /locus_tag="PLL28_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69199.1"
                     /translation="MYKLTILVIGIILISSCTKDPGIGGEATITGKVHALHYNSTFTV
                     LLSEYDLADTYVYLVFGDDINYGTRIKTNYDGEYAFEYLYPGDYTIYTYSIDSAATVN
                     GIPTPPDSAVIRQITISDKNALVETEDLVVFK"
     gene            4603..5151
                     /locus_tag="PLL28_07470"
     CDS             4603..5151
                     /locus_tag="PLL28_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69200.1"
                     /translation="MKIIIAGCIILSSLQLFGQNTRGAKTMYQATREIEAGKQVTVKH
                     VFQSFDNKGRTTEEWTLKGDSTFASRDQWTYNRKGLETKHIIFKDSLNIESIISTSYD
                     RFEVKKLSITTDGKGSIIEQTAYHYNTFGKKIEELTSDSGGNVTKKITFSYDSHGALA
                     ERIIYDGKNNIVSSKQYNYTYR"
     gene            5156..5821
                     /locus_tag="PLL28_07475"
     CDS             5156..5821
                     /locus_tag="PLL28_07475"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HNF69201.1"
                     /translation="MKKILLYLIVMPLCLNVHAQQSDAVFTSGISVEKKITRTWSAST
                     FIQSGFNENYSELGYLLIDGGLGLRLNREWSLEGHYRYGSVRNVANSYDTRQYIYGDV
                     QYVKGIGDFTLIARSRYMVKTYGLYISDENQYRDNKHFLRNRIQLKYDLGPYSDIFLA
                     TEQVYRLDDHNESEQFRFSGGYNYTFNVHHRIQLQYTVAQQVNKKAPDTDYITGVTYY
                     FKF"
     gene            5831..6901
                     /locus_tag="PLL28_07480"
     CDS             5831..6901
                     /locus_tag="PLL28_07480"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HNF69202.1"
                     /translation="MKHYITAICLLAGSLLFTSCDKSSNGGKSDTSTPKFSIAIHGGA
                     GALAKGSYTAEQEAAFKAKLEEATKLGYEMLSKGDSAADVVVAVIKILEDSPLFNAGK
                     GSVITYNGKIKMDAAIMNGRTLDCGTVNNVQRIKNPITAARMVMDSSKYIMFSSAGAE
                     SFAAKNGIEMIDKSYFYTQHQIDRWRKTKDSTDASYIHYVDSMAALQSPIISALPEEG
                     EFGTVGCVVMDKYGNLAAGTSTGGLINKKYNRVGDSPIIGAGTYANNKTCAISCTGTG
                     EDFIKTVAAKTVSDLIEFKGMTLDAATYDMIHVRFKEITGDGGMIAVDKYGNISWQYN
                     SDGMFRARTDQNGKIETYIYAE"
     gene            6941..7873
                     /locus_tag="PLL28_07485"
     CDS             6941..7873
                     /locus_tag="PLL28_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69203.1"
                     /translation="MRIRRYVPANQLIYSSLGLAFILCIFASCSDSKDPVIAYTKSMY
                     VLNKDEEELRSVTDYDSIDRIIAEYIIGRKGRDTVEKSTYVYGDDGLIRNTTFSNDKI
                     THQQFYVYAGSMLMQMIETDGHDTIGITDYVYYPTGKIKRTVLQLVYDKSSPTIIVTN
                     YNRHGDKETMYKQIYEDSSRLVLSRYEMDKFISTYDSSEKMIRNIDQFFLGYYEEADT
                     TLDVSYQYDDAGRLIVERNAKRIDTSVPDSIFYFYNAAGLLQEKLVFTSSAHHREKVV
                     TTDRVQYDYDKNGRLIKEYSFYYKTGRRYSYRAK"
     gene            complement(7922..11065)
                     /locus_tag="PLL28_07490"
     CDS             complement(7922..11065)
                     /locus_tag="PLL28_07490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNF69204.1"
                     /translation="MTKKRPTGRNTLLLALTFASAALSGQSALWTNRFNGTGDNSDRY
                     NQAIADGLGNFYCAGYTVNPGKGKDFYTVKLNSAGDTVWTRTYNYTSNLDDEANYIAL
                     TPAGEIVVCGYSDGGSASTKTDVLTLKYSATGTLLWTARFNEVTTNEDELPAGLAIDN
                     AGNIFIGGRSDHDALNVDDFITLKYTSAGVLSWSARFDNGFTDRAAGIVADQSGGCIV
                     TGRSNNGANDDMLTISYNSLGTVSWSALFAGPGGDDRGQSIARDAAGNVYVAGLRANA
                     SDDDYLTVKYNSSGIEQWTKVFNGGDNDRVSLLKLDGAGNVYVTGQSDIDPSGGNSDY
                     NFRTIKYDPTGVVVWNALSGNPVLQEDIPTDMFIDASGNIYVTGKSDAIGGANVNFEF
                     MTIKYNSAGAQQWAAYFDGTTSNAEDIPATVLTDASSNVWVFGAANFTATQKDCTALK
                     YTAAGVLSATKTINGKGDFNDKVNALTTDASGNSIVVGYTMAAGQQRNILIQKISPAG
                     TTNWTRTFDGTNENDEALAVTTDAAGNTYLAGYSNGSGTYDDYIVIKYSTLGVALWTV
                     IFDHTAHQLDKAVSIVVSDAGDVYVTGYSDGDASALVTNYDITTIKINALGLLQWTAR
                     YNGVGNAADKPAEILFDGSNVLVAGTTFNGANNDIVLIKYDILGTQLAAVTYNGASNG
                     DDAAEDMLIDGSDVYVTGSSFVNTNMDDYVTIKYNSAFVKQWEKNYNGPGDGVDVANG
                     ITVAGAEVYVTGSSVGTSGAADLALIKYNKTTGAQNWAKRYTGAGAFADEGVSVTSDG
                     VDNIYTTGPSGNATSVSDFITLDYNPAGAKLLTLKYNGPGNGEDVAKEVKLDNNGYLF
                     VAGHGTGTGKNNFDFTTIKYCTPVPAASITALGATTICSGSSVTLSANTGTGLTYQWK
                     KGNANIAGATASTYAATTAGGYKVVITNANGCSKTSNVINVSVIICREEQIEIAPVSV
                     NVSPNPFTDYAVIDCSNITMQTEIVVLDITGRTVSTVIAEPGTGSVEIGQDLPQGNYL
                     LVVNHGSQMQTFRIVKM"
     gene            complement(11382..11708)
                     /locus_tag="PLL28_07495"
     CDS             complement(11382..11708)
                     /locus_tag="PLL28_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNF69205.1"
                     /translation="MIFISEPAKHKLESLKSEAGIGSDYFIRVSVVGGGCSGLSYKMD
                     FDNEEKPGDQIFEDRGEKLVTDLKSFLYLCDTTLDFSDGLNGKGFHFVNPNASRTCGC
                     GESFAV"
     gene            complement(11764..12183)
                     /gene="iscU"
                     /locus_tag="PLL28_07500"
     CDS             complement(11764..12183)
                     /gene="iscU"
                     /locus_tag="PLL28_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HNF69206.1"
                     /translation="MAYSKKVLEHYENPQNVGTLDANKQEVGTGLVGAPECGDVMRLQ
                     IEVDPETKVIRDARFKTFGCGSAIASSSVATEWLKGKTIDEALTIDNMDIVEELALPP
                     VKIHCSVLAEDAIKAAINDYRKKNGMPELQLDEKIVH"
     gene            complement(12223..13440)
                     /locus_tag="PLL28_07505"
     CDS             complement(12223..13440)
                     /locus_tag="PLL28_07505"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HNF69207.1"
                     /translation="MLKLPIYLDNSATTPVDPRVVDTMVPYFYEHFGNAASRSHPFGW
                     QAEDAVDQARERVAKLVNASDKEIILTSGATESDNLAIKGVFDMYGKKGNHIVTVTTE
                     HKAVLDTCKHLEKLGAQITYLKTDNEGNIDLNELESVVTDKTILVSVMYANNEIGTIA
                     PIRQIADIAHKKGSLLFTDATQAAGKISIDVERDGIDLMAFSAHKMYGPKGVGALYVR
                     RKNPRVKVTAQMDGGGHERGMRSGTLNVPGIVGFGKAAELAMLEMDADAARLSVMRDR
                     LENALMQMEEAYVNGNRKSRLPHITNISFKYVEGEGLMMAFNQNIAVSSGSACTSASL
                     EPSYVLKALGLGDDLAHSSIRFSLGRFNTDEQVDYTIEAVTKAVNKLREMSPLWEMFK
                     QGIDLSTIEWAEH"
     gene            13728..14129
                     /gene="mce"
                     /locus_tag="PLL28_07510"
     CDS             13728..14129
                     /gene="mce"
                     /locus_tag="PLL28_07510"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HNF69208.1"
                     /translation="MKKIEHIGIAIKALESSNALFAKLFGKDHYKVESVEREGVSTSF
                     FMMGETKIELLEATNPESTIAKFIEKRGEGIHHIAFAVDDIYAEMHRLESEGFQLLHK
                     EPLPGADNKLIAFLHPKSTNGVLVEICMERK"
     gene            complement(14138..15796)
                     /locus_tag="PLL28_07515"
     CDS             complement(14138..15796)
                     /locus_tag="PLL28_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNF69209.1"
                     /translation="MSAVERVIHSPLSTFYKWEATQPDAMYLRQPVNGTWHTYSWREC
                     GEQVRRMAAYLRSLNYAPGSKIAILSKNCVHWMLSDYAIWMAGYVSVPMYPNMAPTTV
                     RQILDHSESKLLFVGKLDDWEYLKPGVPETLPCIAYPFYSVPGFPTWDDIVSKTDPIT
                     DNPDFPMDNLCTIMYTSGTTGVPKGVMHKFYNFAYAAEMAFPALNIDNTAIFFSYLPM
                     CHIAERLLIEMGSLYTGGQVSFAESLDTFPKNLQDTAPTIFLAVPRIWAKFQEKILEK
                     MPQKKLDRMLKIPIIKGVVKTKIKKALGLHNADQVFSGAAPISVDLINWFWKLDIRIQ
                     QAYAMTEDCCYSHTNYKDRNRIGTVGLRFPGVELKFSEQGEMLVKHPAMMVGYYKEDA
                     LTKEVFDEEGFFKTGDKAEVDADGFLKITGRVKELFKTSKGKYVAPMPIEMKFANNTD
                     VGQVLVVGYALPQPIALVTLSENGKKKSKQEVSDDLVKTLKAINATLDSFEKLEKVVV
                     LNEEWTIANNLLTPSLKVKRNEVEKKFQDNYEKWFEMKDIAIWQ"
BASE COUNT         4820 a         3312 c         3138 g         4636 t
ORIGIN      
        1 aagttttgat caggacagaa atttcctgat catgaaaatc agcgcaggag gcactcctgt
       61 ctggacacgc agttacaatg gcacaagtac taagcaaagc actgatgatg cattgtcggt
      121 ggcagtagat gcaagtaata acatttatgc tgcagggttt ctaaaaaaca gtggcacagg
      181 atacgatctt ctggttatga aattatctac agcaggtgat agcttgtggt catacattta
      241 taacgatgca gcctcgaacg aaactgataa agcgctgagt attcaaatga gcccaagcgg
      301 aaatctagtt gtttgcggta gatcagataa tgatctcaca gcggtatcgg atgatgatat
      361 tctggtgatc acattaaata catcaggagg tgtggtatgg acagcacgtt atcagggaac
      421 cggtggaggc gacgacattc cgcgacaaat ggtcgttgcg ccatccggca atatatatgt
      481 tacaggtaaa acaacatcgg ctaccgatat agatgctttg ctgattaaat ataacaccag
      541 cggagttcaa cagtgggtaa gaaaaacgga tatcggggtt gaggatgaat ttacggctat
      601 acaaattgat ggagaggaga atgtattcgt tgccggaaat acaattactg ccacaggtaa
      661 cttcgatgta attacggctt catttgacgc ttccgggaat gagcttttca ccacaactta
      721 tgatcatggt gaaaacggga atgacgaagc gaaaggctta gcgattttcg atgatggatc
      781 atttgcagtt tcatgcacat tggcgcctga tactgatgcg atcacattaa atggagacat
      841 tgctgctgta tattttgata caaatgggaa ctggcaatct acaaccttat ttgatggcgg
      901 tgcaaatgac gacgcatctg aatcagcata cgcatcaaat accggatttg tgatttgcgg
      961 acaaaccgat aacggtgaag caggaaacca agattatgat tatatcacta tttatcttga
     1021 tgaaaccggc accattacga atacagaatt atacaatggt gcggcaggag gtaatgatgt
     1081 agccaatacc ctgatgttat atgacaatgg aacctatata agtggcggaa gtgaaggtgg
     1141 tcagcgagat atagttacta tattatacgg tagcaatcct gtttcgatct ccaacaacgt
     1201 tgcatccaaa aatgtgcagg tttatccaaa tccaaccgtt gcaacatgca ctgtttacct
     1261 cgatgaaatc aatctatcat cttcagctac tttaaaatta tatggaggaa atggcaactg
     1321 tgttatgcaa aaagcaattt cttcaaatga caatatacag ctaacactaa gcaccatgcc
     1381 ggcagggatg tatctgttag tgatcactga cggcgatcag gtctattcta ccaatcttat
     1441 taaacaatga tacttcacct tttaaaaaca gactttatgc aagcgagaat taaaaaatcc
     1501 attatctgca aggaaatcac ttcttatcag atcgacagta cacgtaacaa cacctacact
     1561 ccaattgatg gcgatgttgc tttatttgag gtactggaaa ttggcaaaca ccaatccatt
     1621 caatccgaaa ccaagcgcat agtatccatt tttgaaggtg atgtgatcat ggcagcattt
     1681 gcgaacaggt atgctacatc acaatttgaa ggatatgttc ctgatcgacc tacagaaatt
     1741 cttgacattt tgggtgccgg tggtgctatc ggattggtgg caagcaaaaa tgctgctctt
     1801 gaagatattg agccgacaaa attgcgtttg attggattcg ccactactac agaaggtcaa
     1861 atcatcaata ccaagttttt acatacagca agacacacat ttaatggata tgttcccaac
     1921 aatgccaaaa taatccttag tgtaggctct tccatggatt caggcaaaac gacatccgca
     1981 gcatttactg caaggggctt aaaaacaaca ggcaaaaaag tagcattcat taaattgaca
     2041 ggcaccagtt ttaccaaaga caaagatttt gtatttgatt gtggcgcaga tgtcactgta
     2101 gatttcaccg attgcggata tccatcaaca tatatgtgta gtaaggagga aatacttgat
     2161 gtatatcaat cactgttgat gcaacttgag ccacacgctc ctgattatat tgtaatggaa
     2221 attgcagatg gattactcga acgcgaaact gatttcttgt tacgcgataa agccttcatg
     2281 caaaccatac acagtatgat attcagttgt ggcgacagcc ttggcgtatt ctatggattg
     2341 caattattgc acagttttgg cattgtgccg gctgtaattt gtggccgttt tacgatgagt
     2401 ccgctgctga ttcaagaggt gaaagcaaaa acaaatattc caactttaga tatcgatcaa
     2461 cttgccaatg gtgagtacat tcatgtattc gacaaatcag gcatgcagat tgttcatgct
     2521 gcctaaggaa agtgaatatt attaaaacat attattcccg ccataaaggg ttgctgatca
     2581 gcgtcattgg tttagggttg ttcagtaatg cgttgaaatt ggcaatccct ttatgcgtgg
     2641 gaaaatttta tcagcttgca ttggatggcg gcagtgccag gagtggaata tttgacgcac
     2701 tgtttggcaa tgtgcaatca atcaacattt tcttctctat attttttggc atactgctat
     2761 taagatttat tgttcaattt gcagaacaat atttaatagg catgaccggc gaaaccttca
     2821 gtgcctggtt acgcgaaacg ctcttcacaa aacaattaca taccgtttat tcagtattta
     2881 cgcaaaaaga tccgggaaaa tatctgcttc gctacagcgg tgacatgtcg gctctcagac
     2941 ttttcattac aaaaggacag ataggtgcca taacagattt tgcattcctg ataatgacca
     3001 taggtttatt cggttggttg aatattactt taacatattt acttctgata gccattccgg
     3061 caattttcct gataagcata ctaattaaca gattcatgcg tccttctaca gagaagcgtc
     3121 gtgatatcaa tgcaacgaat ttaaatttcg tgagcgagcg gctgcatgcc atggcaacaa
     3181 taaaggtgat gaatcgcagc acgcccgaaa tcaacaagtt tcaaaggaga tcaacaaaac
     3241 ttttagaagc aggaaaagtt tatcagtttt ggaatgcatt aaacaaggca ctgtatccat
     3301 ttctactata tttattgctg gcgtttgtca tgttgatgat ctataaactg ctaagcaata
     3361 atactattac tgctgacggc ggaacgctac tgatctttgt catgctcatc atccagatca
     3421 ttcctgttgt aaagagactt ttacgttcag gtgctatacg cgaatcagga aaactgagtt
     3481 tgaaaaaatg caagctttta cttgaagcac ctgaagacag cagggaaggt catgcaatgc
     3541 cggttaaaaa aggagatatt cgtataaaca acctaagcat taagtttggt ggtaaaacca
     3601 tttttgaaaa tgcaagtttc catttcccac cgaaaggaat aacattaatt tcaggaacag
     3661 gtggaagcgg aaaatcaacg ctgctgaaat acattagtgg ccttactatt cctgagtcag
     3721 gagaaattat gattgatgac gcatctacta agtcttatag catctttcaa gtcaggaaga
     3781 aaatcacgtt tatatctcaa gagcttccct taataggtaa acatgtatat gatgcaattt
     3841 cctataatcg ggatcctgat aaaaagcatg atgctatgca aatattatca gcaatagggt
     3901 tttgcgacaa atttcaggat gaggttttgg aactgccgtt ggaaaaaggc ggcgccaatt
     3961 tttcaacgag tcagcaacaa ttattgcgca tcgcacgcgg actgatcaca ggaaaaaaaa
     4021 taattctact ggatgaaccc ttcgaaggat tagatgatcg cagtgcacgt caggtagcat
     4081 atcatttaac acatatctca tcctcgcaca ccatcattct tgtttcaaaa agcatacctg
     4141 aaacactttt catacaacac cggatttcac ttgacaagtt aaaacaaccc aatgtataaa
     4201 ttaacaattt tagtaatcgg gatcatactg ataagttcct gtacaaagga tcccggaatt
     4261 ggaggagaag ccaccattac cgggaaagtt catgccttgc attacaacag cacctttacg
     4321 gtattgcttt ctgaatatga tcttgcagac acgtatgtat atcttgtatt cggcgatgat
     4381 attaattatg gcacacgcat caaaacaaac tatgatggtg aatatgcctt tgaatattta
     4441 tatcccggag attacaccat ctacacctat tctattgatt cggcagcaac agtaaatgga
     4501 ataccaacgc cgcctgattc tgcagttatt cgacaaatca ccatttcaga taagaacgca
     4561 ttagttgaaa cagaagatct tgttgtattc aaataaatat ctatgaaaat aatcattgcc
     4621 ggatgtatta tattaagtag cctccaacta ttcggccaaa atacccgcgg agcaaaaacc
     4681 atgtatcagg ctacacgaga aattgaagcc ggaaagcagg ttacagtgaa acatgtattt
     4741 caatcatttg ataataaagg tcgcactact gaggaatgga cattgaaagg tgatagcact
     4801 tttgcaagca gagatcaatg gacctacaat cggaaaggcc tggagacgaa acatataata
     4861 tttaaggact cccttaatat tgaatctatc atctctacat catacgaccg ctttgaagtg
     4921 aaaaaactgt caattaccac agatggcaaa ggttcaataa tcgagcaaac ggcctatcat
     4981 tataatacct tcggaaaaaa aatcgaagag ttaacatcag attccggggg aaatgtcacc
     5041 aaaaaaataa cattttccta cgattcacat ggcgcacttg ctgagagaat aatatacgac
     5101 gggaaaaata atattgtaag cagtaaacaa tacaactaca cataccgata aaagtatgaa
     5161 aaaaatattg ctttacctta ttgttatgcc gctatgctta aatgttcatg cacagcaaag
     5221 cgatgcagta tttacttccg gcatttctgt tgaaaaaaag attacaagaa catggtcggc
     5281 gagtaccttt attcaatccg gctttaatga aaattacagt gaactcggat atttgttgat
     5341 cgatggcgga ctaggattac gactgaatcg tgaatggagc cttgaaggtc attatcgcta
     5401 cgggagtgtt cgcaatgtag cgaatagtta tgatacacga caatatattt atggcgatgt
     5461 tcaatatgta aaaggcattg gcgattttac attgatagcc agatcaaggt acatggtgaa
     5521 aacttatggc ttgtatattt cagatgaaaa tcaatatcgc gacaataagc acttcctaag
     5581 aaacaggatc caattaaagt atgatcttgg tccttacagt gatattttcc ttgcaacaga
     5641 gcaagtatac agacttgacg atcacaatga gtccgagcaa tttcgatttt ccgggggata
     5701 taattacacc ttcaatgtgc atcatcgaat ccagctgcaa tatactgttg cgcagcaggt
     5761 gaacaagaaa gctcctgaca cggactacat taccggagta acttattatt ttaaattcta
     5821 aatttgcttc atgaagcatt acatcactgc aatctgcctg ttggcaggta gtttgctctt
     5881 tacctcttgc gataaatctt ccaacggagg aaagagtgat acatctacac ctaaattttc
     5941 catcgccata catggaggcg caggtgcttt ggctaaaggt tcttatacag ctgaacagga
     6001 agctgcattt aaagctaaac ttgaagaagc aaccaagctg ggatatgaaa tgttgtcgaa
     6061 aggcgatagt gctgccgatg tagtggtagc cgttatcaag attttggaag attcaccttt
     6121 atttaatgcg ggaaaaggct cagtaattac ctataatggt aaaataaaaa tggatgccgc
     6181 tatcatgaat ggacgcactc ttgactgcgg caccgtaaat aatgttcaac gcataaagaa
     6241 cccgatcacc gctgctagaa tggtgatgga ttcgagtaaa tatattatgt tcagcagtgc
     6301 cggagcagaa agttttgccg ctaagaatgg cattgaaatg attgataagt cctatttcta
     6361 tacgcaacat cagattgatc gctggcgcaa gacaaaagac tccactgatg cgtcgtatat
     6421 acattatgta gattccatgg cagcactgca atcgccaatt atttctgctc tgcccgagga
     6481 gggggaattt ggaactgttg gttgtgtggt gatggacaaa tatggcaatc tcgctgcagg
     6541 aacgtcgacc ggtggactta tcaataaaaa atataatcgc gttggtgaca gccctataat
     6601 tggagcagga acctatgcca ataataagac ctgtgctatc agttgcaccg gaacaggtga
     6661 agattttatc aagaccgtag ccgcaaaaac agtgtctgac ctaattgaat ttaaaggtat
     6721 gacattagat gctgcaacat acgatatgat acatgttcgg tttaaagaga ttaccggaga
     6781 tggaggaatg atagcggtag acaaatatgg aaatatctca tggcaatata actctgatgg
     6841 tatgtttcgg gcccgcacag atcagaacgg gaaaatcgaa acctacattt acgctgaata
     6901 gaaggcaatc aatagttact atgtatcttt gtaaatgcta atgcgtatac ggagatatgt
     6961 gcctgcaaat caactgatct acagttctct gggccttgcc tttattctat gcattttcgc
     7021 ctcctgtagt gacagcaaag accctgtaat tgcatataca aaatcaatgt atgtgctcaa
     7081 caaggacgaa gaggaacttc gctcggtgac cgattatgat tctatagata gaattatagc
     7141 ggaatatatt attggtcgca aaggaagaga caccgttgaa aaatcaacct atgtatatgg
     7201 cgatgatgga ttaatcagga atacaacctt ttcgaatgat aaaataacac atcagcaatt
     7261 ttacgtgtat gccggaagca tgctcatgca aatgattgaa acagacgggc atgatacaat
     7321 tgggataaca gattatgtgt attaccctac cggaaaaatc aaacgaacgg tgcttcaact
     7381 agtgtatgat aaatcttctc ccacaattat tgtaacaaat tacaatcgac atggcgataa
     7441 agaaaccatg tataagcaga tttacgaaga cagctctcgt ctggtgcttt cgaggtacga
     7501 gatggataag tttataagca cctatgacag ttccgaaaaa atgatcagaa atatagatca
     7561 attctttttg ggctactatg aagaagcaga tacaacactt gatgtttcct accaatacga
     7621 cgatgccggt cggttaattg tggaacgcaa tgcgaaacga atagatactt ccgttcccga
     7681 cagtatattt tatttctata atgccgccgg tttacttcag gagaaactcg tatttacaag
     7741 tagtgcacat catcgtgaga aagtagtaac aacagacagg gttcaatatg actacgataa
     7801 aaatggtcgc cttatcaaag aatatagttt ctattataaa accggaaggc gctattcata
     7861 tcgggcaaaa taaaaaaagc cggatgcgtg tgcacatccg gcttacagtc caagcaattg
     7921 cttacatttt tacaatccta aatgtttgca tttgcgaacc atgattcacc accaacaaat
     7981 aatttccttg cggcaagtcc tgaccaattt ccacactacc ggttcccggc tcagctatca
     8041 cagtgcttac tgtacgaccg gtaatatcca aaacaacaat ctccgtttgc attgtgatat
     8101 tactgcaatc aatcaccgca taatcagtaa atggattggg agaaacattc acacttaccg
     8161 gtgcaatttc aatttgttcc tcgcgacaaa taatcacact aacattaatc acattggaag
     8221 tttttgaaca gccatttgca ttcgtaataa ctactttata accacctgct gtagttgctg
     8281 cataagtaga tgccgtagca cctgcaatat tcgcgttgcc ttttttccat tgataagtta
     8341 atcctgttcc agtatttgca ctcaaggtta cacttgatcc actgcaaata gtggttgctc
     8401 ccaaggcagt aatagaagcg gccggcacag gtgtgcaata tttaattgta gtaaaatcaa
     8461 aattattttt ccctgttccg gtgccatgac cagccacaaa aaggtatcca ttattatcca
     8521 atttaacctc ctttgcaacg tcttcaccat ttcccggacc attatatttt aatgtaagca
     8581 gttttgcccc agcgggatta taatctaaag tgataaaatc agatacagag gtggcatttc
     8641 ctgaagggcc tgtagtataa atattatcta cgccgtcaga agttacgctt acaccttcat
     8701 cggcaaatgc accggctcca gtatatcgtt ttgcccagtt ttgagcaccg gttgtcttgt
     8761 tgtatttaat caaagctaaa tccgctgctc cggaagttcc aacactgctt cctgttacat
     8821 atacttcagc tcctgcaact gtaataccat ttgccacatc aacaccatca ccggggccat
     8881 tataattctt ttcccattgc ttaacaaagg cactattata ttttatcgtc acataatcat
     8941 ccatatttgt atttacaaat gaagatcctg tcacataaac atcgctgccg tcgatcagca
     9001 tgtcttcagc tgcatcatca ccattagaag cgccattgta ggtaacggct gccaattgag
     9061 tgccgagtat atcatattta attagcacga tatcattgtt tgcaccatta aatgtggtgc
     9121 ctgctactaa tacatttgag ccatcaaaca atatttctgc cggtttatca gcagcattgc
     9181 caacaccatt gtagcgagca gtccattgca ataatccaag tgcattgatt tttatagttg
     9241 taatatcata atttgttaca agtgcacttg catcgccatc tgaataacct gttacataaa
     9301 cgtctccggc atcagaaaca acaatggaaa cagccttgtc aagttgatgc gctgtatgat
     9361 cgaaaataac tgtccacaat gctacaccca gtgtgctata tttaattaca atataatcat
     9421 cataggttcc acttccattg ctataaccgg ccagataagt atttccggcc gcatctgtag
     9481 ttactgcgag cgcttcatca ttttcatttg ttccatcaaa tgttctagtc cagtttgtag
     9541 ttcctgcagg gctaattttt tggataagta tgttgcgttg ctgtccggct gccatagtat
     9601 aaccaacaac gatactattt ccggatgcat cggtggtaag agcattcact ttatcattaa
     9661 aatcgccctt gccatttatt gtttttgttg cgcttaaaac tccggctgct gtatatttta
     9721 atgctgtgca atctttttgt gtagcagtaa aatttgctgc tccaaacacc cagacattag
     9781 aagatgcatc ggttagtact gtggcaggaa tatcttcggc attagaagtt gtgccatcaa
     9841 aatatgctgc ccattgctga gcgcctgcac tattatattt tatagtcatg aattcaaagt
     9901 tcacatttgc accacctatc gcatcgcttt tacctgtcac ataaatattt cctgatgcat
     9961 caataaacat gtctgttggt atatcttcct gcaatacagg atttcctgaa agtgcattcc
    10021 aaacaacaac gcctgttgga tcatacttaa tggttctaaa attataatct gaatttccac
    10081 cggaaggatc aatatcactt tgaccagtta cgtaaacatt tcctgctcca tccaatttta
    10141 ataaactcac tcgatcatta tcaccgccat taaatacttt cgtccattgt tcaatacctg
    10201 aactgttata ttttacagtt aaatagtcgt catctgatgc gtttgcacgt aatccggcca
    10261 cataaacatt tcctgcagca tcacgtgcta tagactgacc tctgtcgtca ccccccggac
    10321 cggcaaaaag tgccgaccat gaaacggtac ctaatgagtt atagcttata gtaagcatat
    10381 cgtcatttgc cccattatta cttcttccgg taacaataca accacctgat tggtcagcaa
    10441 ctatacctgc ggctctgtct gtaaatccgt tatcgaatct tgccgaccat gataaaacac
    10501 ctgcagacgt atatttaagt gtaataaaat catcaacatt gagtgcatca tgatcactcc
    10561 tgccaccaat aaaaatattg ccggcgttat caattgcaag tcctgccggt agttcatctt
    10621 cattggtagt tacctcatta aaccttgctg tccacaacaa ggtacctgtt gcactatatt
    10681 ttagtgttag cacatcggtt ttcgtcgatg cagaaccgcc atcggaataa ccacaaacta
    10741 caatttcacc tgcaggagtt aaggcaatat agtttgcttc gtcatccagg ttggaggtat
    10801 aattataggt gcgtgtccaa accgtatcac cggcactatt cagctttacg gtatagaagt
    10861 cctttccctt gcccggattc acggtatatc cggcacaata aaagttccca agcccgtctg
    10921 caatggcctg gttatatcgg tcggaattat cgccggtgcc attgaacctg tttgtccaaa
    10981 gagccgattg accggaaagg gcggctgaag cgaaggttag tgccagcagg agcgtattcc
    11041 ggccggttgg tctttttttt gtcatttggt agaatttttt cggtaatgaa tcaaaaattg
    11101 gcgcccaaat caagcttttt ggcctgtttg ggttctgcaa ggtcaaaatt gatgtctata
    11161 cacagaaccc ccaacgacag aaaaccaagg cttccattga aggtgtagaa ttacagagca
    11221 gaaaagagcc aaagaaaaca caaaaccctt gtgacagtag ggctgtagat gaaaaaaggc
    11281 aacgacagaa attgatgcct tccgaaaaat cagggcaaaa aaaacctccc ggtcttacga
    11341 tcgggaggtt ttaagttcaa gtatgaaaag aaaagagaat actaaactgc aaaactttct
    11401 ccacatccgc aagttctgga tgcatttggg ttaacaaaat gaaagccttt gccgttcagc
    11461 ccatcagaga aatcgagggt tgtatcgcac aggtagagga agcttttgag gtcagtgacc
    11521 agtttttcac cgcgatcttc aaaaatttga tcgccgggtt tttcttcgtt gtcgaagtcc
    11581 attttatagc tcaatccgga gcaaccgccc ccaacaaccg acacgcgaat gaagtaatcg
    11641 ctgccaattc ctgcctcaga tttgagggat tctaacttgt gtttagccgg ttctgaaata
    11701 aaaatcatag gttttgcatt taccagttac ttaataaacg ccttgatgat agcgctggtt
    11761 cctttagtga acgatctttt catcaagttg caattcgggc atgccatttt tcttgcgata
    11821 atcattaata gccgctttaa ttgcatcttc tgccaaaacg gagcagtgaa tttttaccgg
    11881 aggcaatgct agctcttcaa caatatccat attgtcgatc gtcagtgctt catcaattgt
    11941 ttttcctttc aaccattctg tcgcaacaga gcttgaggcg atagcacttc cgcatccaaa
    12001 tgttttaaat cgggcatcgc gaatcacctt tgtttcagga tcaacttcaa tttgcaaacg
    12061 catcacatca ccacattcag gagctcctac caaaccggtt cctacttcct gtttattggc
    12121 atcgagtgtt cccacgtttt gcgggttctc gtaatgttct aacactttct ttgagtatgc
    12181 cattttatta ttttttaagt atgcctaaat tcaatattat aattaatgtt ctgcccattc
    12241 gatggtgctt aaatcaattc cttgcttaaa catttcccac agaggactca tttcgcgaag
    12301 cttattaaca gcttttgtaa ctgcttcaat cgtataatct acttgttcat cggtgttaaa
    12361 ccttccgagt gaaaaacgta tgctgctgtg tgccaggtca tcacctaaac ccaatgcttt
    12421 cagcacatag ctaggttcca gagatgcgct ggtacaggca cttccggagc taaccgcaat
    12481 attctgattg aatgccatca tcaacccttc accttctaca tatttaaatg aaatattggt
    12541 gatatgcggt aaacgactct tgcgattgcc atttacataa gcctcttcca tctgcattaa
    12601 tgcattctcc aaacgatcac gcattacgct caatcttgca gcatctgcat ccatttcaag
    12661 catggcaagt tcagccgctt ttccgaatcc cacaatgccg ggaacgttta acgtaccact
    12721 gcgcattccg cgttcgtgtc cgccgccatc catttgagcc gtaactttca cccttggatt
    12781 ttttctacgc acatataaag caccaacacc cttcggacca tacattttat gtgccgaaaa
    12841 agccatcaga tcgataccat cgcgttcaac atctattgaa atttttccgg ctgcttgtgt
    12901 agcatctgta aataacaatg atcctttttt atgtgcgata tcagcaatct gacgaatagg
    12961 tgctatcgtt ccaatttcat tgttcgcata catcaccgat accagaattg ttttatcggt
    13021 aactacactt tccaattcat tgagatcaat atttccttcg ttgtcagttt tcagataagt
    13081 aatttgtgca cctaattttt cgaggtgctt acaagtatct aaaactgctt tatgctcggt
    13141 ggttacagtt acgatatggt ttcctttttt accatacatg tcaaatacac ctttgattgc
    13201 aaggttatct gattcagtag ctccggaagt caggataatt tctttatccg atgcatttac
    13261 cagtttggca acacgttccc ttgcctgatc aacagcatct tccgcctgcc agccaaaagg
    13321 gtgactgcgg ctggctgcat tgccaaaatg ttcgtagaag tatggcacca tggtatccac
    13381 cactcggggg tctaccgggg ttgttgcgct attgtcgagg tatatcggga gcttaagcat
    13441 aatttcacta tttagactga ttctaaaaca caaaggtata taaaaggttc gattcagcgc
    13501 actggttttg gaggatttta ggagtgttta tgcccttttt tgcccatttt aaggcgcggg
    13561 gtggcgaaat gggggcgttt tggctgtttt tacctctgaa aacagactcg ttgtggcatc
    13621 cgtcattgct taatatccgt ttaggcttat acctttatcg gacggacgcc ttaggaagca
    13681 attaagcctt ctgagtaatc aacccaatcc gtgctaaaca tatcatcatg aaaaaaatcg
    13741 aacatattgg cattgcgata aaggcactgg aatcatcaaa tgcattattt gcaaaattat
    13801 tcgggaagga tcactacaaa gttgaatctg tagagcgcga aggcgtgagc acttcgtttt
    13861 ttatgatggg agaaaccaaa attgaattac tggaagccac aaatccggaa tctaccattg
    13921 caaaatttat tgaaaaacgc ggagagggca ttcaccatat tgcctttgcc gtagacgata
    13981 tttatgcaga aatgcataga cttgaatcag aaggttttca attattgcat aaagaaccac
    14041 tgcccggggc cgacaataaa ttaattgcat ttttgcatcc aaaatctact aatggtgtgt
    14101 tggtagaaat ttgtatggag cgaaaataga tgcattatta ttgccagata gcaatatcct
    14161 tcatctcaaa ccatttttca tagttatcct gaaatttctt ttcaacttca ttgcgtttaa
    14221 ccttcaatga aggcgtaagc aagttatttg cgattgtcca ttcttcgttg agcacaacaa
    14281 ccttctctaa tttttcgaaa gaatcgaggg tggcgttaat cgcctttaat gttttcacca
    14341 aatcatccga cacttcctgc tttgattttt tctttccgtt ttcagaaagt gtaaccaatg
    14401 cgataggttg cggcaatgca taacctacaa ccaatacctg accgacatca gtgttattgg
    14461 caaatttcat ttcgataggc attggtgcta cgtatttgcc cttgctggtt ttgaaaagtt
    14521 cttttacacg accggtaatc ttcaaaaatc catcggcgtc aacctcagct ttatcgccgg
    14581 ttttaaagaa tccttcttcg tcgaaaacct ctttagtgag cgcatcttcc ttataatatc
    14641 cgaccatcat cgcgggatgt tttaccaaca tttcaccttg ctcgctaaat ttcaattcaa
    14701 caccggggaa acgcaatcca actgtgccaa ttctattcct gtctttataa tttgtgtgac
    14761 tgtagcaaca atcttccgtc atggcataag cctgctgaat gcgaatgtcg agtttccaaa
    14821 accaatttat caagtcaaca gaaattggtg ctgctcctga aaatacctga tcggcattgt
    14881 gcaaccccaa agcttttttt attttcgttt ttacgacacc cttgatgata ggaattttca
    14941 acattctatc aagttttttc tgtggcatct tttccaaaat cttctcttga aatttcgccc
    15001 aaatacgtgg cactgcgaga aaaattgttg gtgcagtatc ctgcagattt ttcggaaacg
    15061 tatctaaact ctctgcaaat gaaacttgtc cgcccgtata taaacttccc atttcaatca
    15121 acaatcgttc ggcgatatga cacatgggga ggtaagaaaa aaatatggct gtattatcga
    15181 tattcaatgc aggaaatgcc atttctgcgg catatgcgaa attgtaaaat ttatgcatca
    15241 cacctttagg cacacctgta gttccgctgg tgtacatgat ggtacataaa ttatccatcg
    15301 ggaaatctgg attatcagta ataggatccg tttttgaaac gatatcatcc catgtaggaa
    15361 aaccgggaac tgaataaaat ggataagcta tacagggtaa cgtttccggg actcccggct
    15421 taagatattc ccaatcatcc agcttgccta caaacaatag cttgctttcg ctatgatcga
    15481 gaatctgcct tacggtagtt ggagccatat tggggtacat gggtacggat acataacctg
    15541 ccatccaaat tgcataatcg cttaacatcc agtgaacgca gtttttcgat aggatggcaa
    15601 ttttacttcc cggggcatag ttcaatgaac ggaggtaggc agccattctg cgcacctgtt
    15661 cgccacattc acgccaggaa taggtatgcc atgtgccgtt gacaggttgt cgcagataca
    15721 tggcatcagg ttgggtagct tcccatttat agaatgtaga cagaggagaa tggatcacac
    15781 gttcaacagc agacatatta tttagtattg tattggtcac taagataata atgacgacca
    15841 taaatgttaa atctttcagc aactttgcac tatgaacgag caatcaggac gtacggagat
    15901 atcttc
//
LOCUS       DAOQHY010000072        15854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1751_length_15854_cov_6.447876, whole genome shotgun sequence.
ACCESSION   DAOQHY010000072 DAOQHY010000000
VERSION     DAOQHY010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15854
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1751_length_15854_cov_6.447876"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PLL28_07520"
     CDS             <1..215
                     /locus_tag="PLL28_07520"
                     /inference="COORDINATES: protein motif:HMM:NF040058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69210.1"
                     /translation="RHEVFNDKNSARYLTYEDIFEMRLFNSVVVKEDNVYDRNIESYA
                     TGIDQVLESERIKQQIFEFEHTLWEF"
     gene            complement(279..2093)
                     /gene="uvrC"
                     /locus_tag="PLL28_07525"
     CDS             complement(279..2093)
                     /gene="uvrC"
                     /locus_tag="PLL28_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNF69211.1"
                     /translation="MDLDAFKQIEINIPGNPGVYRYYDKEGTLLYVGKAKNLKKRVSS
                     YFQKTDHTARIKLMVKKIAKIEFTIVNNEADAFLLENTLIKKHQPRYNVNLKDGKTYP
                     YIVVKNERFPRIFLTRQKRQDGATYLGPYTSVGKVKTVFDFIKSVFPIRTCNFHLSEQ
                     NIQAKKFKVCLEYHLGNCKGPCEGLMQEADYNENVQQIMHILKGNVGSVKVAFKKQMQ
                     QYVEQLQFEQAELMRKKLESVEAFQGRSTVVNTSISNVDVFGYTDAESFAVIGYMKIM
                     QGMVTQATTLVLTRKLDETPEELLQIAVMELRLQFESDTPEIIVPFQIEWIDEKLVQT
                     VPQAGDKKKLLELAFKNAMYVREEKEAGKQKREDRNQNTRVLEAIKADFKLTQLPYHI
                     ECFDNSNIQGTNPVASMVCFKNAKPSKKDYRHFNIKTVEGPNDFASMHEIVHRRYKRL
                     IEEAIPLPQLIVIDGGKGQLGAAVDALKELGIYGQVAICSIAKRLEEIYFPEDPLPLY
                     INKKSESLRVIQQLRDEAHRFAITFHRQKRSKSAIKSELDDIKGIGRATASNLLKAFK
                     SVKNIRNASIEALSEVVGQAKAQIIIDHFHTTGESSEA"
     gene            complement(2195..4492)
                     /locus_tag="PLL28_07530"
     CDS             complement(2195..4492)
                     /locus_tag="PLL28_07530"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNF69212.1"
                     /translation="MSSTTIKEQFSTYKRFIRWFWIIFGSGSLLVIIFILLLSAGVFG
                     ALPSFEELENPQSNLATQILTSDGELLGRFYRENRTNATYDEIPPFLIDALVSTEDRR
                     FYEHSGIDAKSVMRVIFKTILLHQEESGGGSTLTQQLAKNLYGRPVASTKFELGLIKF
                     KEWITAAKLERRYTKEEIIVMYLNTIPYIYDASGVKTAAKTFFNKSIQDISVEEAAVL
                     VGMVQNPSFYNPVRFPDRCRDRRNIVLHEMHEANKLSDEDYQKAKESPLVLNFKRQDH
                     KAGSGTYFREYLRQWLADWVEENPKPDGGKWDIYKDGLKIYTTIDSRMQAYAEQASQE
                     WLTKLQTQFDNHWKGNKYEPWAYPYSGRTNPELINKAMKQTDRYRGLKKAGKTDAEID
                     KIFHTPDSMTVFSWKSKNYDLDTIMSPYDSIKYVKKMLQPGFMAMDPHTGGIKAWVGG
                     IDMRYFQLDHVKPSHGRQVGSTFKPFVYTAAIADKGFSPCYQIPNLPVSFEKGEKWGL
                     LSDWTPKNSDGHYGGMLTLSEGLGKSVNTVTAFLLREVGIQPVIDLSKNMGIESEIPY
                     QPSICLGTPEVTVYEMTAAYSAFANKGVAVRPVFVTRIEDKNGNIIAEFPAKRVEVMR
                     EEVASVMLNMMEYVVNSGTGQRIRRPEYGGITSEVAGKTGTTQENTDGWFMGMVPQLV
                     CGTWVGADDPVVHFRSTALGQGANMALPIYGLFMKRVYADKTLGIDPNAHFPAYEGTR
                     TIELDCGKYRDNQRNDYQDLMEGYN"
     gene            complement(4601..7117)
                     /locus_tag="PLL28_07535"
     CDS             complement(4601..7117)
                     /locus_tag="PLL28_07535"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF69213.1"
                     /translation="MTRSVDDLIARDNGYFNARLIMRESEKNLWEEQQDNYEEILPIF
                     KYGTQEQATAIQPGMDEVNTKCSWVIQKHKKSKWVDDCYFLLGKAYFYERNYDEALTS
                     FQYIVAEYSNLIEKQSKNKRISEDEDGELSFFEKLKHQPVSSEAGIWVARSLVEKKKF
                     SEAHTVISVLKSKENFPKWLLGELYAVEADGYMKQGQVENAVEPLSEAVANTEDKVLK
                     ARYLFILAQLYAQQHDTKNAMKAYQEVLQLKPSYDMDFYTRLNMAKLSLENSSLSGRE
                     SKEDLLALLKDEKYKDYYGLIYYTLADIAFADKDQDMGMQYLNLCIRSTNDGKQRGLA
                     YMRLGDIYFSEPEYKTAYAYYDSTVNTLPSEHERFKEIKSLRDNLKLLVDELTIIETE
                     RKLQYWAGLSDKDLMKELEKLVPEVEEKDSLEANVTDPNNVTPLTPGITDGDFYFYNT
                     TLRSRGFSEFKKTWGTRKLEDNWRRSEKGSFGEEGETAVEGTGEEQSIDLTGKNITID
                     KIKQSLPTTPEMIAQSNARIAGALYRAGMIYKDNFRDKAKAIGSFEDNVNNFEGNEYE
                     IHCLYQLYRLTSAPAQNNYKQKLLDKYPESDYARIITDPGFYERKEAEKHALEDYYAV
                     TYDLLQKRNFAGVKSRVVAADSLFKVNPLKPKFDMLNALADADSLDLFKNSLQKVALK
                     YPEHEVGVRAQEILDYLRRGSALEDAGKGIVTANYTHDATEEHYFIYVMSGTGKNATT
                     MKNNVATFNSINFSSDNLKISSLLLGKENSIILVKSFNNQDKAMIYYYAIKDNPGVYK
                     DLAKDGFLPVVISKTNYVQFFKVKDVEGYEAYFQENYLDK"
     gene            7365..8177
                     /locus_tag="PLL28_07540"
     CDS             7365..8177
                     /locus_tag="PLL28_07540"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNF69214.1"
                     /translation="MHDKTFEVQASLRLSPLNVIILSSTLFVVIGLIVYMAIVFTPLK
                     NLVVGYSEVTTSRQIAENKRVTDSMLTTLQAYDMYFNNLQKRLRGETDSIIPIDSLKK
                     TDYSKIVVDEAGETDLAMREQIEREDLFNLSISDPRETTGAQNLESLHFFPPIKGTVT
                     SNFQPSNKHFGIDVVAPENTPVKACLDGVVIDSYWNFESGNVVVIQHDHGLISFYKHN
                     SQILKRSGNKVRAGDVIAIIGNTGELSSGPHLHFELWHDKTPLDPKEFINFN"
     gene            8192..8608
                     /locus_tag="PLL28_07545"
     CDS             8192..8608
                     /locus_tag="PLL28_07545"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNF69215.1"
                     /translation="MFANKTNTPQSTPGSYISNRINHGTVMEGKLDSDGDIRVEGIIR
                     GSIRTSAKVAVGSTGLIEGDIHCKSADIEGRIIGDVEVTEVLTLKSTAIVEGNIYTAK
                     IVIENGAHFDGICNMGTKEKERRITHAQPELEEAAI"
     gene            8616..8801
                     /locus_tag="PLL28_07550"
     CDS             8616..8801
                     /locus_tag="PLL28_07550"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNF69216.1"
                     /translation="MKYSGMAFQLFAGIFLGVWGGIKLDAWLNTKPWFTVILSLFGIA
                     AGMYSVLKDFIDSGKKK"
     gene            8831..9211
                     /locus_tag="PLL28_07555"
     CDS             8831..9211
                     /locus_tag="PLL28_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69217.1"
                     /translation="MIIFAVVLAIVLLILGMVSEFKPAMTFAWMCFGMFFALTLITYF
                     FSAKTMERKFSSFMNVFFVGIFSKLILSAIMVLIFKVKNETTGLNYIVPFAIIYFTFL
                     FFETVELVKLSRSIGNPPPQKPTK"
     gene            9213..11153
                     /locus_tag="PLL28_07560"
     CDS             9213..11153
                     /locus_tag="PLL28_07560"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNF69218.1"
                     /translation="MRIPGFLLLLCSPFFIKAQTPDLHAFEKSMVFAEQQAAAGKISV
                     QSTAPTNNYDLIYQKCAWTIDPDTLFIDGTITSQLVLTEESNAIYFDISDSLKVDAVT
                     VNGVEGDFLTANNAVQINLDDTYDIGAEFEIAVTYHGTPSTNGFGSFKKTSQDAGNVI
                     WTLSEPYGASDWWPCKQSLTDKLDSVDVWITMPDNCKSGGPGLLDTITEAGIGWHTYR
                     WKHRYPIATYLIGISVTDFDEFSFYAPHHEDSVLFYNLVYPSSFDEAVTQINGIVPAF
                     EIFSDIYGDYPFADEKYGHMQFGWGGGMEHQTMSSVSGYSHELLVHEMAHQWFGDKIT
                     CASWHDIWLNEGFATYSTWLSYDLGDDPNNYYEAWLKTTRDKVVSLPDGSVYVDDTTL
                     VSRIFDGRLTYYKGGWVLHMLRWELGDDAFFAGIKNYITDPAFSFKFVTTQDLISHLE
                     EAADTSLTEYFDDWFYGQGFPEFQIIWQQDTLGHFIMSVSQTPTHESVDFFNMHVPLR
                     LYGNGDSLDLRLNHTVNGELFEMDLPFMVDSIVLDPELRILHANDAIVHLPITEGILQ
                     AVIYPNPVSDILHMQILEPSASTYNLTIWNAAAEKVVSAEFNTSGSSASYTIDSTSWP
                     SGLYMVLIKSGEKEVVQSVVVQ"
     gene            complement(11154..12422)
                     /locus_tag="PLL28_07565"
     CDS             complement(11154..12422)
                     /locus_tag="PLL28_07565"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNF69219.1"
                     /translation="MNSLKLVLILLLACLHGLIQAQQNFQPAIIFSKTGDTIPGYIDY
                     RQWDKSPETIHFRQNEESPTINMNPLTIAGFSVSGECYISSVIGLDGTPYTYRELPRT
                     ITQKIIIDTVFLKTLVLSDGGMDLYSLKDSRGKQHYFIHDSGSMTELIDIKYYSESEQ
                     SGIVMHVYKDQLYEWMEECPPAAKASLDADLKTDDLIEAITNFDSCLAVPIDYISPIL
                     PLKKVFGLAIGYGINRVTYPISMVSTGYGTADFNWGPTYYGALFYDIDMASNRESFWL
                     CFDGGIAQNKSSGTYFGEIGNSHESGSFTFSALETLVSTQFKWQSNAPHFKPYLKAGY
                     LATFEIQQSNSMHTEKYNIYVPDVVTVTDEENSINYRKFANHIVGQTGLSINNKYFIE
                     LTYSTGLTKGADDFVSINRDRLTLGLKYGI"
     gene            12485..13648
                     /gene="purT"
                     /locus_tag="PLL28_07570"
     CDS             12485..13648
                     /gene="purT"
                     /locus_tag="PLL28_07570"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF006766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HNF69220.1"
                     /translation="MKKIMLLGSGELGKEFVIACKRLGHYVIAVDSYNDAPAQQVADT
                     REVISMLDGDALDAIVKKHEPDIIVPEIEAIRTERFYDYEKWGIQVVPSAKAANFTMN
                     RKAIRDLASKELGLRTAKYAYALSFEELKTAVAEIGIPCVVKPLMSSSGKGQSVIKTE
                     ADIQTSWDYVQAGKRGDIAEVIVEEFIRFDSEITLLTVTQKNGPTLFCPPIGHRQERG
                     DYQESWQPALIKPEHLLEAQHVAKQVTEALTGAGIWGVEFFLAETGVYFSELSPRPHD
                     TGMVTLAGTQNLSEFELHARAVLGYPVPEITLERAGASAVILAEKESNSFSIVGVEKA
                     MQEVNTDVRIFGKPTTRKYRRMGVALACGKPDTNTDALRKKAMINASKVEIKY"
     gene            complement(13693..15462)
                     /locus_tag="PLL28_07575"
     CDS             complement(13693..15462)
                     /locus_tag="PLL28_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF69221.1"
                     /translation="MKQFAFYLLILPLLLHTPLDAQNNFLKRSIYFDSNKYNLDREDS
                     ITLSLIADSIQNYASYRLLIRGNTDADADSAYNIKLSQNRCNTVQQFLLLNGAKISNL
                     ELDWYGENKPIAENETESGKQQNRRVDIIVYKIIRKPKNPITDSVYVPTDNIEDLFAL
                     LKQEPQQYCINPTRDTVLVGEQGTILYFKANSIAVPKGIDTCVVICLTEMFRKSDMIL
                     NNVTTLANGKILESQGMIKVEAKVGGASLDLNKGADYIVMIPTDTIVPGLSVFNGDEA
                     HDMLNWLVNTNADLRNFTPEMLIGCGDWLCGYLQGCSRCKYMFNKPANFFCRCKRVDE
                     GFRGIFSNAQHQRNRSFRVCQRILRKTERQNRKIDRQIKKGNFNPPAFTGIDTSSPTI
                     DYSFDRCQRIIDLFNKYGVDNIAALVDAINQPMYEKYGVTNINDLQEALRKEEQEKKE
                     ARLRDMEFKYNDKNISFEDLNYYIYNQNALGWSNIDKIIGNNNQVEFKVNLKASEDTN
                     VKLVFKNVRSVVPAFIKNGKYVFEKIPGGMEATLIIIQYKNDKVYMAFQDITTSNDKI
                     EVEFKEYTLEELKAKLKELDMLN"
     gene            complement(15510..>15854)
                     /locus_tag="PLL28_07580"
     CDS             complement(15510..>15854)
                     /locus_tag="PLL28_07580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69222.1"
                     /translation="QIDDEELLILYRTERNIAYPTADLSPECPIITDCKELNVSFDGL
                     SKHLSIHIAPVGMAPTGVNYSIYNMLGQLVYNSLGNELHEVDCHSFAKGSYIVNATLD
                     GEIFCNQQFVVY"
BASE COUNT         4427 a         3188 c         3193 g         5046 t
ORIGIN      
        1 tacgtcatga agtatttaat gacaaaaaca gcgctcgtta cctgacctat gaagatatct
       61 tcgaaatgag attgttcaac agcgtagtcg ttaaagaaga taacgtttat gatcgcaata
      121 tcgaaagcta tgctaccggt atcgatcagg tgttggaaag cgaaagaatc aaacaacaga
      181 tcttcgaatt cgaacacacg ctctgggaat tttaattacc ttgcttttct attcatactt
      241 gaaccgaagg ccgatctaat aagtcggcct tcactttttt atgcctccga tgactcaccg
      301 gtggtatgaa aatgatcaat gatgatctgt gccttcgcct gacctacaac ttcgcttagc
      361 gcttcaatgc ttgcattgcg aatattcttt accgacttaa aggccttcaa taaattactc
      421 gccgtagccc ggccaattcc tttaatgtcg tcaagctctg atttaatagc cgacttactt
      481 cgtttttgtc tgtgaaaagt gatggcaaaa cgatgggctt catcgcgaag ctgttgtatt
      541 acccgcaaac tctcactctt tttattgata tacaagggta ggggatcctc cgggaaatat
      601 atttcttcaa gtcgttttgc aatactgcaa attgctacct gtccataaat gccaagttct
      661 tttaaggcgt ctactgcggc gcctaattgt cctttaccac catcaataac aataagttgt
      721 ggcaatggga tggcctcctc tatcagacgc ttgtatcgac gatgaacgat ctcatgcatg
      781 ctggcaaaat cgttgggtcc ttcaactgtt ttgatattga aatgacggta atctttttta
      841 ctgggcttgg catttttaaa acataccata ctcgcaaccg gattggttcc ctggatattt
      901 gagttgtcaa aacattcgat atgatatggt aattgagtga gtttgaaatc ggctttgata
      961 gcttcaagta ctcgtgtatt ctggttgcga tcttcgcgct tttgctttcc cgcttccttt
     1021 tcctcgcgca catacatcgc atttttgaat gcaagctcta ataatttctt tttatcgccg
     1081 gcttgaggaa cggtttgcac taatttttca tctatccatt caatttgaaa cggaacaatg
     1141 atttcaggtg tgtcactttc aaattgtaaa cgcaattcca taactgcaat ttgaagtaat
     1201 tcctccggcg tttcatctag ttttctggtg agcactaagg ttgttgcctg tgttaccatg
     1261 ccctgcatga tcttcatata tccgattacg gcgaaagatt cggcatctgt atagccgaac
     1321 acatctacat tgctaatgct tgtattcaca acggtgctgc gcccctggaa ggcttctaca
     1381 ctctccagtt tttttcgcat taattcagct tgctcaaatt gcaattgttc tacatattgc
     1441 tgcatctgtt ttttaaatgc aactttaaca gatccgacat ttcctttgag gatgtgcatg
     1501 atctgttgca cattttcatt gtaatcagct tcctgcatca acccttcaca tggaccctta
     1561 caatttccca ggtgatactc gagacatact ttaaattttt tcgcctgaat attttgttca
     1621 ctcaaatgaa agttgcaagt gcgaatcgga aatacagatt ttatgaagtc aaaaactgtt
     1681 ttcaccttgc caacagaggt ataaggtcct aaataagtag cgccatcttg tcgcttttgg
     1741 cgtgtaagga aaatgcgcgg gaatcgctcg ttttttacaa cgatatatgg atacgttttt
     1801 ccgtccttta aattgacatt gtatcttggt tgatgttttt taatcagggt gttttccaat
     1861 agaaaggcat cggcctcatt attcacaata gtgaattcga tctttgcgat ctttttcacc
     1921 atgagtttta tccttgccgt atgatcggtc ttttgaaaat acgacgaaac gcgcttcttg
     1981 agattttttg ccttccccac atataacaag gtgccttcct tgtcgtaata acgatatacg
     2041 ccgggatttc ccgggatatt tatttctatt tgtttgaaag catcaagatc catagctggt
     2101 cagtcggctg cataaagtta atcgtaaacg gcaaaaaaaa tcgccgacag caaaagtaaa
     2161 ctgtcggcga tgagtttatt gcttcgctat tatgctaatt atatccttcc attaggtcct
     2221 ggtaatcatt gcgttgattg tcgcgatatt taccacaatc caattcaatg gttcgtgtgc
     2281 cttcgtatgc agggaaatgt gcattggggt ctatacctaa tgttttatcg gcatagacgc
     2341 gtttcataaa taaaccatat atcggcaatg ccatattcgc gccttgcccc aatgccgtgc
     2401 ttctgaaatg gacaaccggg tcatcagcac ctacccaggt tccgcaaacg agttgtggta
     2461 ccattcccat aaaccatcca tcggtatttt cttgtgtagt tcctgttttc cctgcaacct
     2521 cgctggtaat gcctccatat tccggacggc gaatgcgctg tcctgtaccg ctatttacta
     2581 catactccat catattgagc ataacggatg ccacttcttc gcgcatcact tctacgcgtt
     2641 tggcaggaaa ttctgcaatg atattgccgt ttttatcttc aatacgtgtt acaaaaacag
     2701 gtcgcacagc aacgccttta tttgcaaaag cactataagc ggcagtcatt tcataaacag
     2761 ttacttccgg tgtgcctaaa caaatggaag gctgatatgg aatttccgat tctatgccca
     2821 tgtttttaga aagatcaata accggttgaa ttcccacttc tcgcaataaa aatgcagtaa
     2881 cggtattcac cgattttcct aatccttcgc taagcgtaag catgcctcca taatgaccgt
     2941 cagagttttt tggcgtccag tcgctgagga gtccccattt ttcgcccttc tcaaatgata
     3001 cagggagatt aggaatttga tagcagggag aaaatccttt atctgcaata gccgccgtat
     3061 atacgaaagg tttaaatgtt gaacctacct ggcgaccgtg agaaggtttt acgtgatcga
     3121 gttggaaata gcgcatatca ataccaccta cccatgcttt gatgccacct gtatgaggat
     3181 ccattgccat aaatccgggt tgaagcattt tctttacata cttgatggaa tcgtacggcg
     3241 acattatcgt atcgaggtca taattcttgc tcttccagga aaatactgtc atcgaatccg
     3301 gagtgtgaaa aatcttatcg atttcagcat ccgttttccc tgcttttttt aatccgcgat
     3361 agcgatcggt ttgtttcatc gccttgttaa tcaattccgg attagttctg ccgctgtaag
     3421 gataagccca aggctcatat ttatttccct tccagtgatt gtcgaattgc gtttgcaatt
     3481 tggtgagcca ctcctgcgat gcctgctccg catatgcttg cattcggcta tcaatggtgg
     3541 tgtaaatttt taatccgtcc ttataaatat cccattttcc accatcaggt tttgggtttt
     3601 cttccaccca atctgcaagc cattgacgta aatattcgcg gaaataagtt ccacttcctg
     3661 ctttatgatc ctgacgtttg aaatttaata ccagtggcga ctcttttgcc ttttgataat
     3721 cttcatccga taatttattg gcttcatgca tttcatgcaa tacaatattt cttcggtcgc
     3781 gacaacgatc aggaaagcgc acaggattgt agaatgaagg attctgcacc atccctacaa
     3841 gtacggcggc ctcttctaca gaaatatctt gaatagattt gttgaagaac gttttggcag
     3901 cggtttttac accactcgca tcgtagatat acggaatggt atttagatac attacaataa
     3961 tctcttcttt cgtataccga cgttctaatt ttgctgccgt gatccattct ttgaatttga
     4021 taagccctaa ttcaaatttt gtagatgcaa caggtctccc atataagttt tttgccagct
     4081 gctgagtaag tgtactacct ccaccggatt cttcctggtg taacagtatt gttttaaata
     4141 tcacacgcat cacactcttt gcatctatac cgctatgttc atagaacctg cgatcttctg
     4201 tagatacaag tgcatcaatc aggaatggcg gtatctcatc ataggtggca ttggttcggt
     4261 tctcgcgata gaatcgtcct agcagttcac cgtcgctcgt gagaatttgc gtggcaagat
     4321 tactctgcgg attttccaat tcctcaaagg aaggcaatgc accaaaaact cctgccgaga
     4381 gtaataaaat aaaaataatg accaataatg accctgagcc aaaaatgatc cagaaccaac
     4441 gaataaatcg tttgtaggtc gaaaattgct ccttgatggt agttgaggac atgcctgtaa
     4501 gttaaattgt gattggaggg acaaactcta aattacaaaa gtatcgctgt tgccatactg
     4561 aaacgcggta tgcttccaca aattaaatgg ggaattccaa ttatttgtcc agatagtttt
     4621 cctggaagta agcctcatag ccttctacat ctttaacctt aaagaattga acataattag
     4681 tcttagagat cacgacagga aggaatccgt ctttagccag atctttatat acacccggat
     4741 tgtctttgat ggcataataa tagatcattg ctttatcctg attgttgaag gattttacaa
     4801 gtatgatact attttccttg cccaataaaa ggctactgat ttttaaatta tcagagctga
     4861 aattaatact gttgaaggtg gcaacgttat tcttcatggt ggtagcattt ttacctgttc
     4921 cactcatgac ataaatgaaa taatgttcct ctgtagcgtc gtgggtgtaa ttggctgtaa
     4981 caattccctt tccggcatcc tccaaagcgc tgccgcgacg aaggtagtcc agtatttcct
     5041 gtgcacgaac gcctacttcg tgttcaggat attttaaagc aactttttga aggctatttt
     5101 taaataaatc caagctatct gcatcagcta atgcattgag catatcaaat ttaggcttga
     5161 gaggatttac tttgaataaa ctatctgcag caactacgcg tgattttaca cctgcgaaat
     5221 tacgtttttg taaaagatca tatgttactg cgtagtagtc ttctaaggca tgtttttctg
     5281 cttcctttcg ctcatagaaa cccggatcgg tgataatgcg tgcatagtct gattcgggat
     5341 atttatctaa aagtttttgc ttgtaattgt tttgtgcagg agccgacgtt aatcgataaa
     5401 gttgatatag acaatgtatt tcatattcgt tgccctcgaa attattgaca ttatcctcaa
     5461 atgatcctat cgctttagcc ttatcacgga aattatcctt gtaaatcatg ccggcacgat
     5521 aaagtgcacc tgcaatccga gcattacttt gagcgatcat ttcgggagtg gttggtaaac
     5581 tctgcttgat cttatcaatt gtaatatttt ttcctgtcag gtcgattgat tgttcttctc
     5641 ctgttccttc aactgctgtt tcaccttctt caccaaagct gcctttttcg cttcttcgcc
     5701 agttgtcttc caattttctt gtgccccagg tttttttgaa ttctgaaaat cctcgggagc
     5761 gcagggtagt gttataaaaa taaaaatcac catccgtgat gcccggtgta agaggtgtta
     5821 cattattagg atctgtaaca tttgcttcca ggctgtcttt ttcttcaacc tccggaacta
     5881 acttctccaa ttctttcatt aaatctttat cggaaagtcc ggcccagtat tgaagtttgc
     5941 gctcagtttc aataatagtt aactcgtcta ccaataactt taaattgtcg cgaagtgatt
     6001 ttatttcttt aaagcgttca tgttcactcg gcaaagtatt tacggtgctg tcgtaatagg
     6061 cgtatgccgt tttgtattcc ggttcgctga aataaatatc gcctaaacgc atataggcta
     6121 atccgcgctg ctttccatca ttggtactgc gaatacatag attcaaatat tgcataccca
     6181 tatcctgatc cttatcggca aaagcaatat cagctagagt ataatatatt agtccataat
     6241 aatctttata tttttcatct tttagcaatg cgagtaaatc ttcttttgat tcacgaccgc
     6301 ttaaagagct attttcaaga ctcaactttg ccatattgag gcgagtataa aaatccatat
     6361 cataggaagg ctttaattgc aaaacttcct gataggcttt catggcattt ttggtatcgt
     6421 gttgttgagc gtacagttgt gcaaggataa acaagtagcg cgccttcaat actttgtctt
     6481 cggtgttggc aacagcttct gataaaggtt caacggcatt ttccacttgt ccttgtttca
     6541 tgtagccatc agcttcaacg gcgtataatt ctcccaataa ccatttgggg aaattctcct
     6601 tgcttttcaa tacgctgata acggtatgtg cttcactgaa tttctttttc tcaactagtg
     6661 agcgggcaac ccatattccg gcttcgctgg atacgggttg gtgtttgagt ttttcaaaaa
     6721 aacttaattc gccatcctca tcctcagaaa tacgcttgtt ttttgattgc ttttcaatga
     6781 ggttggaata ttcggcaacg atatattgaa atgaagtaag cgcctcatca taattgcgtt
     6841 cgtagaagta ggccttgccc agcagaaagt aacaatcatc tacccacttg ctttttttat
     6901 gtttttgaat gacccacgaa cacttagtat tcacctcatc catgcccggt tgtatggcag
     6961 tagcctgctc ctgggtacca tacttgaaaa tggggagtat ctcttcgtag ttatcctgct
     7021 gttcctccca taggttcttc tcagactcgc gcatgatcag gcgggcgttg aaatacccat
     7081 tgtcgcgagc tatgaggtca tcaacactcc gcgtcacgat atttcggtcg gcatttttac
     7141 tcgaactgca agccgtaacg aaaaaaagaa gtaaaattgt gactggaatt aggactgagg
     7201 atcgtttcaa ggttactagt tttataccgg aataattaac tctattacgg caaaattatt
     7261 ttaattaaag cgaaaattac agcatttcag cgtcaatggc caaagggggg gtgaaaacta
     7321 acaaattatg ggaccggata cggacgaaat accgactggt tttgatgcac gacaagacct
     7381 ttgaagtgca ggcctcgctg cgtttgagcc ccttgaacgt gatcattctt tccagcacac
     7441 tttttgtagt gattggcctt attgtttaca tggcaatagt cttcaccccg ctgaagaacc
     7501 tcgttgtggg ctatagtgag gtcacaactt cgagacaaat agctgagaat aagcgagtta
     7561 cggatagtat gctcactact ttgcaggctt atgacatgta ttttaacaac ctccagaagc
     7621 gtctgagagg tgaaacggat agcataatac ctatcgatag cctcaaaaaa acagattata
     7681 gcaagattgt ggtagatgag gccggtgaaa cggatttggc tatgcgcgag caaattgaaa
     7741 gggaagacct tttcaatctc agcatttcgg accctcgtga gactacaggt gctcaaaatt
     7801 tggaatcgct gcactttttc cctcccatca aaggaaccgt tacctcaaac ttccagccct
     7861 caaacaagca ttttggtatt gatgtggtgg ctcctgaaaa cacccctgta aaggcatgtt
     7921 tagatggggt ggtaatagac agctattgga attttgagag cggtaatgta gtggtgatac
     7981 agcatgatca tgggctcatt tcattttata aacacaacag tcagatactc aagcgctcag
     8041 gtaataaggt acgtgccgga gatgtaatcg ccatcattgg aaacacgggt gaattgagca
     8101 gcggccctca tttgcacttt gaactttggc acgacaaaac tcctcttgat ccgaaagaat
     8161 tcattaactt caactaaaat ttcatagcct tatgtttgct aacaaaacga acacccccca
     8221 gtccacacca gggagctata tttcaaacag gatcaaccat ggtaccgtga tggaagggaa
     8281 gctagacagt gacggtgata ttcgtgtgga aggaatcata cgcggatcaa taagaacatc
     8341 ggcaaaggtt gcagtgggtt ctaccggact tatcgaaggc gatattcact gtaaaagcgc
     8401 tgatatcgaa gggcgcatca tcggtgatgt ggaggttacg gaagtgctta cactgaaatc
     8461 aactgcgatt gtagaaggga atatttatac tgcaaaaatt gtgattgaaa acggcgctca
     8521 ttttgacggc atatgtaata tgggcacgaa agaaaaggaa cgcagaataa cacatgccca
     8581 acccgagctc gaagaagccg caatttgata gctacatgaa atattccgga atggcatttc
     8641 agctgttcgc aggaattttt ttgggcgttt ggggaggaat aaaactcgat gcgtggctga
     8701 acacaaaacc ttggtttact gttatacttt cattgttcgg aatcgcagcc gggatgtatt
     8761 ctgtattgaa agattttatt gattccggaa aaaagaagta gtagttcata tgttgaaatt
     8821 tgtagtccgc ttgataatct ttgccgttgt tttggcaatc gtgttgttga ttttaggaat
     8881 ggtatccgaa tttaaacctg caatgacctt tgcatggatg tgtttcggaa tgttttttgc
     8941 actcactctg atcacttatt ttttttctgc aaaaacgatg gagcgaaaat tcagcagttt
     9001 tatgaatgtc ttctttgtag gcattttttc aaaacttatt ctttctgcca tcatggtact
     9061 catttttaaa gtgaagaatg aaactacagg attgaattat atcgttcctt tcgcaattat
     9121 ctactttacg ttcttatttt ttgaaaccgt agagttagtg aaattgtcga gaagtattgg
     9181 taatccgcca cctcaaaaac caacgaaata atatgcgaat tccggggttc ttgctgttgc
     9241 tctgttcccc cttttttatc aaagctcaga ctcctgatct gcatgctttc gaaaagtcaa
     9301 tggtttttgc agaacaacaa gctgctgccg gcaaaatatc agttcaaagc acagcgccta
     9361 caaataatta tgatttaatt tatcaaaaat gtgcctggac catagatcct gatactttgt
     9421 ttattgatgg cactattact tcacaacttg ttttaaccga agaaagtaat gcgatttatt
     9481 ttgacatttc tgactctttg aaggtagatg cagtaactgt aaatggggtg gaaggagatt
     9541 ttctgactgc caataatgct gttcaaatta acttagatga tacgtacgat ataggtgctg
     9601 aatttgaaat tgcagttact tatcatggca caccatcaac aaatgggttt ggctccttta
     9661 agaaaacatc acaagatgcc ggcaatgtaa tatggacact ttcggagcct tatggcgcaa
     9721 gtgattggtg gccctgtaaa caatcactta cagataaatt ggattcagtt gatgtatgga
     9781 ttaccatgcc cgataattgt aaatcaggtg gtcccggatt gctcgatacc attacagaag
     9841 ctggaattgg atggcatacc taccggtgga aacaccgata tccgattgct acttatctga
     9901 ttggaatatc tgttactgat tttgatgaat ttagtttcta tgctccgcat catgaagact
     9961 cagtgctgtt ttataatctt gtgtatccgt caagttttga tgaagcagtt acacaaatta
    10021 atggcatagt gcctgctttt gaaatttttt cagacatcta tggtgattac ccatttgccg
    10081 atgaaaaata tggtcacatg caattcggat ggggtggggg aatggaacac caaacgatga
    10141 gttctgtttc ggggtatagt catgaattat tggtgcatga aatggcacat caatggtttg
    10201 gtgataaaat aacctgcgca agctggcatg atatatggct gaatgaagga tttgctactt
    10261 atagcacttg gttgagttat gatcttggcg acgacccgaa taactattac gaagcatggt
    10321 tgaagactac acgcgataaa gtagtatcat tacctgatgg cagcgtttat gttgatgata
    10381 ccacgttggt tagccgtatt tttgatggca gattaaccta ttacaaaggt ggttgggtgc
    10441 tccatatgtt gcgttgggaa ttgggtgatg atgctttttt tgcaggcata aaaaattata
    10501 taaccgatcc tgcattttca tttaagtttg ttactacgca agatcttatc agtcatctcg
    10561 aagaagctgc tgacaccagc ctgaccgaat actttgatga ttggttttat ggacaaggat
    10621 ttccggagtt tcaaataata tggcagcaag atactttagg acatttcatt atgtctgtat
    10681 ctcaaacgcc aacacatgaa tcagttgatt ttttcaatat gcatgtgcct ttgcgattgt
    10741 atggaaatgg tgattcactt gatttgcgac tcaaccatac cgtaaatggt gaattatttg
    10801 aaatggatct tccgttcatg gttgatagta ttgttttaga ccctgaattg cggatattac
    10861 atgccaatga cgccattgtt cacttgccaa taacagaggg tatattgcag gcagttattt
    10921 atcccaatcc tgtatctgat atcttgcaca tgcaaatttt agagccgtcc gcttcaactt
    10981 ataaccttac aatttggaat gctgctgctg aaaaggttgt gtcagcagaa tttaatacta
    11041 gcggatcttc ggcttcctac acgattgatt caacatcttg gccatccgga ttatacatgg
    11101 tactgatcaa atccggtgaa aaggaggtag tgcagtctgt tgtcgtacaa taattaaata
    11161 ccatacttta atccgagcgt cagcctatct ctatttatag atacaaagtc atccgctcct
    11221 ttggtcaatc cggttgaata ggtgagctca ataaaatatt tattattaat cgaaaggccg
    11281 gtctgtccga cgatatgatt ggcaaatttg cgataattaa tactgttttc ttcgtctgtt
    11341 accgttacaa catcaggtac gtatatattg tatttttcgg tatgcatact gtttgactgt
    11401 tgaatttcaa atgttgctag atatccggct ttaagatatg gtttgaaatg aggtgcatta
    11461 ctttgccatt taaattgtgt tgacaccaat gtctccagtg ctgaaaaagt aaatgatcct
    11521 gattcatgac tattccctat ttcaccgaaa tatgtgccag atgatttatt ttgtgcaatt
    11581 ccaccatcaa aacatagcca aaaggattcg cgattactgg ccatatcaat gtcatagaat
    11641 aatgcaccat aatatgtcgg gccccaatta aaatcggcag tgccatatcc ggttgaaacc
    11701 atggatattg gatacgttac cctgttaatg ccatacccta ttgcaagtcc gaatactttc
    11761 tttaatggca gtattggtga aatatagtca ataggcactg ccagacaaga atcaaaattt
    11821 gtaatggctt cgataaggtc gtcggtttta agatctgcat cgagagaggc tttggctgct
    11881 gggggacatt cctccatcca ttcataaagc tgatccttgt aaacatgcat gacaatgcct
    11941 gattgttcac tttcactata atattttatg tcaatcaatt cagtcattga accactatca
    12001 tgtataaaat aatgttgttt ccctcttgaa tctttcaatg agtacaagtc cattcctcca
    12061 tctgaaagca caagtgtttt taaaaagacc gtatcgataa tgattttctg agtaatcgtg
    12121 cgaggcaatt cgcgataggt atatggagtc ccatcgagcc caattacaga gctaatatag
    12181 cattctcctg aaactgaaaa tccagcaata gttagcgggt tcatattaat cgtcggactt
    12241 tcctcattct ggcgaaaatg aatagtttca ggcgatttat cccattgtcg ataatcaata
    12301 taacctggaa ttgtatcccc cgtttttgaa aatattattg ccggttgaaa attttgttgc
    12361 gcctgaatga gaccatgtag acatgctagc aaaaggatta aaacgagttt caagctgttc
    12421 atgaggcatt aataattgtg tatattcagt aaaaatccga aattgcctcc aaataattca
    12481 atatatgaaa aaaatcatgc tccttggtag cggtgaactg ggaaaggaat tcgttattgc
    12541 ctgcaagcga ttaggccatt atgtaattgc tgtagatagt tataacgatg ctcctgcaca
    12601 acaggttgct gatacgcgtg aagtcatttc aatgcttgat ggtgatgcgc tggatgcgat
    12661 tgtgaaaaag catgagcctg atatcatagt tcccgagatc gaagccattc gcaccgagcg
    12721 attttatgat tatgaaaagt ggggtattca ggtggtgccc agtgctaaag cagcaaattt
    12781 tactatgaac cgtaaggcca tccgtgatct tgcttcaaaa gagttgggat tgcgtacagc
    12841 gaaatatgcc tatgcattga gtttcgagga attaaaaact gctgttgcag aaattggaat
    12901 tccatgtgtt gtaaagccac tcatgagttc cagtgggaaa ggtcagagtg taattaaaac
    12961 agaagcagat atccaaacct cttgggatta tgtccaagcc ggaaaacgtg gtgatattgc
    13021 agaagtgatc gtcgaagaat tcattcgttt tgattctgag atcacattac ttacagtaac
    13081 tcaaaaaaac ggccctacct tattttgccc gccgataggt caccgacaag aacgcggcga
    13141 ttatcaggag agctggcagc ctgcactaat aaaacctgag catctcctgg aagcccaaca
    13201 tgtcgctaaa caggtaaccg aagccctcac cggcgccgga atatggggtg tggaattctt
    13261 cctcgcagaa acaggcgtat atttttcaga attaagtccc aggccgcatg ataccggaat
    13321 ggtgacatta gcaggaacgc aaaatctttc ggaatttgaa ttgcatgcac gtgctgttct
    13381 cggttatccg gttccggaaa tcactttgga aagggcagga gcaagtgcag taattctggc
    13441 tgaaaaagaa agtaatagtt tcagcattgt tggtgtagaa aaagccatgc aggaagtgaa
    13501 tacagatgtc agaatattcg gtaaaccaac cacacgcaaa taccggcgaa tgggtgtggc
    13561 gctggcttgc ggcaaaccgg atacaaatac agatgcattg cgaaaaaagg ctatgatcaa
    13621 tgcatcaaag gttgagatta aatactgaca ttacagataa aaagggagcg tgaaagcagc
    13681 atctacctca tttcaattaa gcatatccaa ttccttcaat ttagccttta attcttcaag
    13741 cgtatattct ttaaattcaa cttctatttt gtcattgctt gttgtaatat cctgaaacgc
    13801 catgtatact ttatcgttct tatattgaat aattataaga gttgcttcca ttccgccggg
    13861 aattttttca aagacatatt tcccattttt aataaaagcg ggaacaacac ttctcacgtt
    13921 tttaaagaca agtttcacat tggtatcttc acttgctttg agatttactt taaattcaac
    13981 ttgattatta tttccaataa ttttatcaat attagaccag ccgagagcat tttggttgta
    14041 tatgtaataa ttcagatcct caaatgaaat atttttatca ttatatttga attccatgtc
    14101 tcgtagacgt gcttcctttt tctcttgttc ttctttgcgc agcgcctctt ggaggtcatt
    14161 tatgtttgta acgccatatt tttcatacat cggttgatta attgcatcaa ctaaagcggc
    14221 aatattgtcg acgccatatt tattaaataa atctataatt ctttgacacc gatcaaagga
    14281 gtaatcaatt gtaggactgc ttgtatcaat acctgtaaac gcaggtgggt tgaaattgcc
    14341 ttttttaatt tgcctgtcta ttttgcggtt ttgtctttct gttttccgta aaattctttg
    14401 acaaacgcgg aaactcctgt tacgctgatg ttgcgcattg ctgaatattc ctctaaaacc
    14461 ttcatcaact cgcttgcagc ggcaaaagaa attcgccggt ttattaaaca tgtacttgca
    14521 tcgcgaacat ccttgaaggt aaccacatag ccaatcgccg caacctataa gcatttcagg
    14581 tgtaaaattt cgtaaatctg catttgtatt gacgagccaa ttcagcatat catgtgcttc
    14641 gtcgccattg aaaacagata atcccgggac tatcgtatca gtaggaatca tgacaatata
    14701 atctgctcct ttattcaggt ctaaggaggc tcctcctact tttgcctcaa ctttaatcat
    14761 accttgtgat tccagaattt ttccattggc aagcgttgta acattattga gtatcatgtc
    14821 cgactttctg aacatttctg tcagacagat aactacacat gtatcaattc ccttgggaac
    14881 ggcaattgaa ttcgctttga aataaagtat tgtgccttgt tcacccacga gaacggtatc
    14941 tctggttggg ttaatgcaat attgttgagg ttcctgtttc agtagggcaa acaagtcttc
    15001 aatattgtct gtaggaacat atactgagtc tgtaatagga tttttaggtt ttctgataat
    15061 cttgtagacg ataatatcca cgcgacgatt ttgttgtttg ccgctttctg tttcattttc
    15121 ggcaataggc ttattctctc cataccaatc caattctaaa ttggagattt tcgcgccatt
    15181 caatagcaga aactgttgaa ctgtattgca tctgttttgc gataacttga tattataggc
    15241 actgtcggca tcggcatctg tattgccgcg gattaacagc ctgtaactag cgtagttctg
    15301 aatagaatca gcaatcaatg acagggtgat gctatcttct ctgtcgagat tatatttgtt
    15361 gctgtcaaaa taaattgacc gttttagaaa attattctgg gcgtcgagcg gtgtgtgtaa
    15421 taatagcggt aaaatgagga ggtaaaaggc aaattgtttc atagtggagg gagttggtct
    15481 gctaaattta ataaattgtt agtagagcgc tagtaaacaa caaattgctg attgcaaaag
    15541 atttcaccat caagtgttgc atttacaata tagctgccct tcgcgaagga atggcaatct
    15601 acttcatgca attcattacc gagagaattg taaactaatt gccccaacat gttgtaaatg
    15661 gaataattaa ctcctgtggg agccattcct accggtgcaa tatggatgct aagatgcttg
    15721 ctaagcccgt caaaagatac gtttagttct ttgcaatctg ttattattgg acattcaggt
    15781 gagagatcag cggttggata ggcaatattt ctttctgtgc ggtataaaat tagcaactcc
    15841 tcatcgtcga tttg
//
LOCUS       DAOQHY010000073        15849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1752_length_15849_cov_5.346334, whole genome shotgun sequence.
ACCESSION   DAOQHY010000073 DAOQHY010000000
VERSION     DAOQHY010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15849
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1752_length_15849_cov_5.346334"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2242
                     /gene="pbpC"
                     /locus_tag="PLL28_07585"
     CDS             <1..2242
                     /gene="pbpC"
                     /locus_tag="PLL28_07585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HNF69223.1"
                     /translation="FLLLNLAFPMHVKIDYSQTVTAADGTVLHAFLTTDDKWRMMTEL
                     SEITPEMQKAIIFKEDKYFELHPGVNPLAICRALFNNIRTGHRTSGASTITMQVARML
                     EPKDRTYGNKVVEMFRALQLDMLYSKEEILQMYLNLVPYGGNIEGVKAASILYLQKMP
                     AVLSLSELTALSIIPNRPTSLVPGQNNAQIIVERNKWLEKFRNADLFSDDIIDDALAE
                     PFNAKRTEIPQLARHFSTRMRMEFPADPIIKTTLQPQLQSQCEEVMSNYVGQLWALDI
                     NNGAAIVIDNKSHKVVAYVGSANFFNERDGGQVDGVQAVRSPGSTLKPLLYAMAFDKG
                     IYTPQSIVSDVPINYTGYRPENYDEDYHGNVSITYALANSLNVPAVKTLHEIGMEDFL
                     NMLQRAGFSSLTNQRKDLGLSVALGGCGITLEELTRAYSAFPNNGKSPHIRYLQSDND
                     TTATPVFTEAAAFMTTEILSELTRPDLPLFYQNAKKVPRVAWKTGTSYGRRDAWSIGY
                     NEQYTVGVWVGNFSGRGVPELSGANVATPLLFNIFQVLGSDVDADWNRMPESVQFRYV
                     CEVSGEIPTANCSHVVMDYFIPGISKNDSCTHLIDVMVDPKETISYCNTCKPETGYKV
                     KQYQNYSPQIISWMQEKHLPFESIPPHNPKCERMFSDQMPSITSPADQMEYLIEKDDP
                     QRIMLSANVASDVKTIFWYINDKLLTSVNAGEPVFFDPAEGKTKISCSDDKGRNVDIE
                     IMVEFF"
     gene            complement(2498..3886)
                     /locus_tag="PLL28_07590"
     CDS             complement(2498..3886)
                     /locus_tag="PLL28_07590"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HNF69224.1"
                     /translation="MKKIIMILALPAIIMAQGCSGWVDEVSTSPNSPTEVTPDLLLSV
                     SEVAMQTLYTGQLTRTASILTQQCAGNDFQMIDIRDYKITELSNTNEWKTVYADALIN
                     QQTLIDIAGDENPYYRGIARVLKCMTIGIATDFWGDVPYSQALQGLDGTDAALNPEYD
                     AQADIIAAMQSELDAAIADLSMPSTSNAFLPASDDFIHNGDVSMWIKAAHMMKARYAN
                     RLSKRDATGSANEALMHIAESGVTSNADNTNAIFGTNGNELNPWFSFNTTRANYIKMG
                     AFFIDLLSSTNDPRLPLYATTDDGGGYSGVATNSVDPAASNTGELYSSDASPLPLLTY
                     YEMKFIEAEANLRLGNNDAAAAALNDAVKANILATVGAPDPVYEAAHASETGASITLD
                     KIMTQKYIAMFTQPESWADWRRTNIPALTPNPDAIYDEIPRRFPTCIDERLYNSNVQV
                     VGDITLPVWWDE"
     gene            complement(3905..7078)
                     /locus_tag="PLL28_07595"
     CDS             complement(3905..7078)
                     /locus_tag="PLL28_07595"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNF69225.1"
                     /translation="MRKLAASVLLLMFGMVSVAFGQGKTITGTVTSATDNQPLPGATV
                     MVKGTTVGTVTDLDGKYVLVVPQDKDVLVFTFLGFKTLEESIGSKNVVNIAMDEDVLG
                     LETVVVTALGIPKEKLSLGVSTQEVGGDRLASSGEENLVEALSAKAAGIQVVGSAGTP
                     GASAKIIIRGPSTFTNENQPLIVIDGVPIDNSTTSTVAGDYPFNPTLNGVSNSNRAID
                     INPDDVESINILKGPAAAALYGSRAGSGAIIITTKRGNKAGDKAVHINFSSSLEVSQV
                     NKIPAQQQLYAQGSGGGFTDADGNVDPEGNYITSDPGPDFLWGTDDDGSAGTANSWGP
                     LMSNIGITPTDNPGEFFQNGMTYNNNLSVSGGTDRGSLRLSIGNTQDKGIVPNTQFDR
                     TSVRVNSDMRIYDKLKVAVDANYITSGGTRAQNGSNLSGVMLSLLRTPTSYDLGAGYE
                     YPSGDNRNYFSAYDNPYWTVNNNPYTDNVNRLLSNVSATYTPWTWLNVTYRVGADNYT
                     DLRKQIFSIGSNDPANAPGGQIEENTIRYRQIYSDLIATATHQVNEDLGITLTVGNNL
                     TDIDINNQYSRGRDLTIPDFYNLSNAADLYSSQYTTKQRNAAFFGDLEFDYRELLFLT
                     LTGRDEFSSTYGPNQSSAFFPSASVAFVFSDILTTNNIWSFGKLRFAYAQAGIEPQPY
                     SADTYFIPPLYTDGFTDGYSFPYLGQSGFGYSQLNTLGNPDLSPERLTGTEFGLELKF
                     WKGRIDLDAVYYIQESSDILLTKPIASTSGFSYVYDNAGAMTNKGIELTLNADIIKKK
                     NLTWNVGGNFSKNENEVTQLADGVDEIEIEAGFGDPGAYVIVGQPYGLLYGSQWAYNE
                     DGDLLIDPSSGLPIFDTVSGVIGDPYPDWLMNISTALTYKGFTVSGLLDIRKGGDIWC
                     GTIARMNRIGISEASGDRTETYVIDGVIENADGTYSQNDIAISPADYWQSYQGDFGAS
                     SQAVYDGGWVRLREVKLAYEFNLDKKNVIKGLTVAATGRNLFLKTDYPGVDPETSLTG
                     AGSNISGFDWFNNPGTKSYLFTVSAAF"
     gene            complement(7256..7981)
                     /locus_tag="PLL28_07600"
     CDS             complement(7256..7981)
                     /locus_tag="PLL28_07600"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HNF69226.1"
                     /translation="MFRNFCLAAITMYLFTGATCDPQVMEDVLNTVMSTTITDQEASN
                     GLIEALVQGASNGSATLAKVDGYLKNPSVKIPFPPEAQKIETTLRDLGLNKMCDDVIN
                     SVNHAAEDAAAEAKPILVNSIRSMTFSDAMNILMGADDAATEYLKKTTTAQLTAKFKP
                     VIDKSLSEVGATKYWSDAVNYYNKIPLVEDMNPNLSDYVTQKALDGLFYMIEQEELKI
                     RQDPAARASEIVQKVFGYYDTHK"
     gene            8116..9372
                     /locus_tag="PLL28_07605"
     CDS             8116..9372
                     /locus_tag="PLL28_07605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HNF69227.1"
                     /translation="MRAALISIGDEILNGTTINTNASWISTVLQPLGVDIHEVLAISD
                     HADHILGTLHAYVGEMEIIIITGGLGPTKDDITKKTLCRYFDSTLEFHQDIYDRLKAA
                     FAKRNIPFTENNRDQAMYPHNCEVLHNALGTAQGMLFKRNGSVIVSLPGVPFEMKGLM
                     TDQVLPRLREELQFPTLLNQYFMTSGISESFLARQLEPLEAMLPGHIGLAYLPSPGVV
                     KLRLTARGSDAATLKAELEMLSNEMRRILGSGIYAEEPISIEEHLGKLLVEGKATLGC
                     AESCTGGKIAHKITAIPGSSNWFKGGVIAYDNQLKINILQVNESIISQFGAVSEETVS
                     AMLDGALTVLGTDFAIATSGIAGPTGGTADKPVGTVWVGVAGGGKKVVHKYFFNKNRE
                     INIEYACMFALHEMRLLLTEILVKPS"
     gene            9459..10760
                     /locus_tag="PLL28_07610"
     CDS             9459..10760
                     /locus_tag="PLL28_07610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNF69228.1"
                     /translation="MAQKELIMPKMGESIMEATILKWLKKEGDTVSVDEAVLEIATDK
                     VDSEVPSPFAGTLTKILFKEGDVVQIGKAIAILDGDGSVTTKTAAPEPAVKTEVVVET
                     PKVVAPVVQTEVPVVAGDRFYSPLVLNIAREEGISMAELERIPGTGKDNRVTKGDILN
                     YVQNRGAGTTQAPVEQQAPVTQTKTEEPVIVKTASVSGNVEIREMDRMRKLIADHMVM
                     SKRVSPHVTSYVEVDMTPIVEWRNRIKGKFEQREGQKITYTPIFIEAIAKAIKDFPGI
                     NISVDGNNIIYKKDINIGMAAALPSGNLIVPVIKNADRMNMVGLTMVVNDLANRARAN
                     KLKPEEIQDGTFTLTNVGSFGSLMGTPIINQPQVAIMAVGAIKKRPVVLETTQGDVIA
                     IRHMMFMSMSYDHRVVDGMLGGSFLKRVADYLEGFDTEREL"
     gene            10794..12185
                     /locus_tag="PLL28_07615"
     CDS             10794..12185
                     /locus_tag="PLL28_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNF69229.1"
                     /translation="MYIEQLYTNCLAEAAYYIESNGEAAIIDPLRDTAPYIEKAASRG
                     AKIKYVFETHFHADFVSGHIDLARQTGAQIVYGPEANTSYDVVNAADGEIFHVGSLQI
                     RALHTPGHTLESTCYLLIDANGKDHCIFTGDTLFVGDVGRPDLLDGIMTKEELASKMY
                     DSLNNKIKPLADDVVVYPAHGPGSACGKSMGKETFSTIGIQKQTNYALQPMTRDEFIA
                     AVTDGLSQPPKYFFEDARINKQGYEDVEMVMAKNAKPLSITAFETAVESGALILDTRI
                     ADIFEKAFIPNALNIGLNGMFAVWVGTLVDIDQPLVLVTEPGKESESVLRLARVGYEN
                     VLGFLEGGIDAWIQSGRTIETVTTIDPGSIKPYMGNDDYIVLDVRKPSEFENEHISGA
                     INIQLSEIGARLHELQPNKQYIMHCAAGYRSMMAASILKANGLNNFVNVRNGWTGIKK
                     TDLPTESSVPELN"
     gene            12205..12603
                     /locus_tag="PLL28_07620"
     CDS             12205..12603
                     /locus_tag="PLL28_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69230.1"
                     /translation="MRKLILIIAVIISFTSCDNFKESEAIEHITRTWTVYKYEVDGYD
                     QTPLFVATYGTYTLVCEKDQDYTETYTVGATPVTITGIYIFSDGDNILTLSDEFQSRV
                     FNVLTLTNNEMTLEDRNADTNQVYYLEAVI"
     gene            complement(12655..13296)
                     /locus_tag="PLL28_07625"
     CDS             complement(12655..13296)
                     /locus_tag="PLL28_07625"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNF69231.1"
                     /translation="MKKTSVLLLLAVLMHSAVFGQSDRKKYKHLPDGLYAEVQTTKGN
                     ILLFLEFQKAPLTVANFVGLAEGKIENTAKPLGTPYYDGLTFHRVIADFMIQGGDPSG
                     NGSGGPGYAFKDEFHSDLRHTGPGILSMANAGKFTNGSQFFITHKETPWLDNKHAVFG
                     HVVEGQDVVNAIQQGDKINHIKIIRKGKAVKKYDPAAVFKSFEDKGDVKRQGK"
     gene            complement(13312..14361)
                     /locus_tag="PLL28_07630"
     CDS             complement(13312..14361)
                     /locus_tag="PLL28_07630"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNF69232.1"
                     /translation="MVTLVPELTMELTNLFNWLKQPRRITLLTHVRPDGDAIGSALAM
                     KMYLEKKGHTANVIAPDDFPDFLDWMPGSKQVYIHQRNRKTCFAILTQSDAIFFLDFN
                     AIKRIEALGEDVMKLTRPYVSVMIDHHREPDNFTDYRLWNDHASSTAELVYTFMEMAG
                     DIHLLDQDMAACIYAGIALDTGIFQYSNTTAHVHAIAGKLMEYGIKVDRIHNDLYNQY
                     GENRIRFIGYLLSQKLQIIPAYRAAYMSITMAEAEQYKLSIGDKEGIVNLPLAMKDID
                     IAVLFTEDKGKVKMSLRSKGDVNVEILARNFFNGGGHMNASGGSSKQSLEDTIKTFEE
                     VLPKFYAMPEANTNQ"
     gene            14425..14844
                     /locus_tag="PLL28_07635"
     CDS             14425..14844
                     /locus_tag="PLL28_07635"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNF69233.1"
                     /translation="MAGNITLTMIKPDAVKKNYIGPILKMINEAGFRIVAMKYTKLSK
                     EKAGEFYAVHKERSFYGELVDFMSSGPIVAAILEKENAVEDFRKLIGATDPSKADEGT
                     IRKLYAASIGENAVHGSDSDENAAIEGDFHFSKLERF"
     gene            complement(15046..>15849)
                     /locus_tag="PLL28_07640"
     CDS             complement(15046..>15849)
                     /locus_tag="PLL28_07640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69234.1"
                     /translation="VRFDNAFVTSNNADAATGGAFGEWRVNLSNTPEVGMRCDDYSYE
                     LPFEFGTDSLAMGQSLSYIQGLMYYSFSNWKLLPRDKNDIDGYHTTYPNSIVLFNFTT
                     PVASGAIDQAAGTITLTVPVGTDVTALVPTIDYTGQYVDPVGGVAQNFTSPVNYTCFS
                     PVDYTPKTYVVTVNFGVSATALAMAEMTVSPNPATDEMTVEIDMPSASDITFDLLDLE
                     GRKVLTTSELCTVGKNIFRFDLRGLSNGLYLLQLSSSTETTIRKIEVSR"
BASE COUNT         4645 a         3236 c         3424 g         4544 t
ORIGIN      
        1 gtttctgctg ttgaacctgg catttccgat gcatgttaag attgattatt cgcagactgt
       61 aactgcagct gatggaacag tactgcacgc atttctgacc actgacgata aatggcgcat
      121 gatgaccgag ttgtcggaga tcacgccgga aatgcaaaaa gcaataattt ttaaggagga
      181 taaatatttc gaattacatc ccggagtaaa tcctcttgcg atatgcagag cgctgttcaa
      241 taatattcgc accgggcacc gaacatcagg ggccagtacc atcaccatgc aagtagctcg
      301 tatgctggaa cccaaggatc gcacttacgg aaataaagtt gttgagatgt tccgcgcctt
      361 gcaattggac atgctgtatt ctaaagaaga gatattgcaa atgtatttga atctggttcc
      421 ttatggcggt aatattgaag gcgtaaaagc tgcatccata ctttatctgc aaaaaatgcc
      481 ggcagtatta tcactttcag aattaacggc attgagcatt attcctaatc ggccaacctc
      541 actggtaccc ggtcaaaaca atgcacagat cattgttgag cgaaataaat ggttggaaaa
      601 attcagaaat gcagatttgt tttcagatga catcatagat gacgcgcttg cagaaccgtt
      661 taacgcaaag cgaactgaaa ttccgcaact ggcaagacat ttcagcacac gtatgcggat
      721 ggaatttcct gccgatccaa ttattaaaac tacactccaa ccacaactgc aatcgcaatg
      781 tgaggaagtc atgagtaatt atgttggtca attatgggcg ctagatatca ataatggggc
      841 tgcaatagtc attgataata aaagtcacaa agtggtggct tatgttggtt ctgcaaattt
      901 ctttaatgag cgtgatggcg gtcaagtaga cggtgtgcaa gcagtccggt ctccgggaag
      961 tacgttaaag ccgctattat atgcgatggc ttttgataaa gggatataca cacctcaatc
     1021 tattgtaagt gatgtcccga ttaattacac cggttaccga ccggaaaatt atgatgaaga
     1081 ctatcatgga aatgtttcta taacttatgc actcgcaaat tctttgaatg ttccggctgt
     1141 aaaaaccttg catgaaattg ggatggaaga ttttctgaac atgttacagc gcgcaggatt
     1201 ttcatcactc accaatcaaa gaaaagatct tggattatct gtagcattag gaggttgtgg
     1261 tataacgctg gaggaattga cacgtgccta ttccgcattt ccaaataacg ggaaatcgcc
     1321 gcatatcagg tatttacaat ccgacaatga tacaaccgca acgcctgtat ttacggaagc
     1381 cgccgcattc atgactacag aaattctgag cgaattaacc cgacctgatc ttccattatt
     1441 ttaccagaat gctaagaaag ttcctcgtgt tgcctggaaa accgggacat cctacggtcg
     1501 tcgggatgcc tggagtatcg gctataatga acaatatacg gttggcgtat gggttggcaa
     1561 tttcagtgga cgcggtgttc cagaattgag tggtgcaaat gtggctacac cattgttatt
     1621 taatattttt caggttttag ggtcagatgt tgatgcggac tggaatcgca tgccggaatc
     1681 tgttcaattc agatatgtat gtgaagttag tggtgaaatt cctactgcaa attgttcgca
     1741 tgtagtaatg gattatttca tacccggcat ttcaaaaaat gatagttgca cacatttaat
     1801 agatgtaatg gttgatccga aagaaactat aagctattgc aatacttgta aacctgaaac
     1861 gggttataaa gtcaaacaat atcaaaatta ttctcctcag atcatcagct ggatgcagga
     1921 aaaacatttg ccatttgaaa gtattcctcc acacaacccg aaatgtgaac gcatgttcag
     1981 cgatcagatg ccttcaatta catctcctgc tgatcaaatg gaatatttga ttgaaaaaga
     2041 tgatcctcag cgcatcatgt tgtcagccaa tgttgcttca gatgtcaaaa ccatattctg
     2101 gtatatcaac gataaattac tcacttcagt taatgcagga gaacctgtat ttttcgatcc
     2161 cgctgaagga aaaacaaaga tcagttgcag tgatgataaa ggcaggaatg tcgatatcga
     2221 gatcatggtc gagttctttt gagggaaatc gtgagtgggt cagtgggtca gtgggtcagt
     2281 ggtgagtggt gagttgacag gtatcaggta aaaattcacg actcaccact caccactcac
     2341 cactcaccac tcaccactca ccactcacga ttcacgactc acgagtcacg attcacgatt
     2401 ggtagcaagt agtgtgcagc ttgatacatt taataatcaa aaagccccac tcaatagagc
     2461 agggcttttg tctttttatt aaagtgttat gatttaatta ttcatcccac caaacaggta
     2521 aagtgatatc acctacaact tgcacattgc tattgtagag acgctcatca atacaagttg
     2581 ggaagcgacg aggaatttca tcatagatcg catccggatt tggtgtcaat gcaggaatat
     2641 ttgtgcgacg ccagtcggcc caagattcag gctgagtgaa catagcgata tatttctgcg
     2701 tcatgatctt atcaagtgtg attgaagcgc cggtttcact tgcgtgagct gcttcataaa
     2761 caggatcagg agcacctacc gtggcgagaa tattcgcttt cactgcatcg tttaatgcgg
     2821 cagcggcagc atcattattg ccaagacgga ggttagcttc agcttcgatg aatttcattt
     2881 catagtaagt gagcaatggc aatggtgatg catcagatga atacaattca ccagtatttg
     2941 atgcagcagg atccacactg tttgtagcta cacctgagta tccgccacca tcatccgtag
     3001 ttgcatacaa aggcagacgt gggtcgttgg ttgaagataa taagtcgata aagaatgcac
     3061 ccattttgat gtaattagcg cgagtggtat tgaaagagaa ccaagggttc aattcatttc
     3121 catttgtacc aaagattgca ttcgtgttat ctgcattaga ggtaacacct gattcagcga
     3181 tatgcatcaa tgcttcatta gcagaacctg ttgcatcgcg tttgctcaag cgattggcat
     3241 aacgagcctt catcatatga gcagctttga tccacatgct tacatcacca ttatggatga
     3301 aatcatccga agcaggcaag aaagcatttg aggttgatgg cattgataaa tcggctatag
     3361 ctgcatctag ttctgattgc atagcagcaa tgatatctgc ttgtgcatca tattcaggat
     3421 tcaaagccgc atcggtacca tcgagtcctt gaagcgcttg agagtatggc acatcgcccc
     3481 agaaatctgt agcgattccg atagtcatac atttcaatac gcgtgcaatt ccgcgataat
     3541 atggattttc atcaccggcg atatcaatca atgtttgttg attgattaaa gcatcggcgt
     3601 atacagtttt ccattcgttg gtattactta attcagtgat tttataatca cggatatcaa
     3661 tcatttggaa atcgttaccg gcgcactgct gtgtcagaat agaagcggtg cgggtaagtt
     3721 gcccggtata aagtgtctgc attgcaacct cagaaactga gagcaacaga tcgggcgtta
     3781 cttctgtcgg agagtttggc gaggtactta cctcatcaac ccagccgctg caaccctgtg
     3841 ccataatgat ggcagggaga gcaagtatca ttataatctt tttcataatt cagattttaa
     3901 tggtttagaa agcagcgctt actgtgaata aatatgattt agttccaggg ttattaaacc
     3961 agtcgaaacc tgagatgttt gaacctgcac ctgtaaggct tgtttcagga tctacacctg
     4021 ggtaatcggt tttgaggaag aggttgcgac ctgttgcagc cactgtaagg cctttgatca
     4081 cattcttctt atcgaggttg aattcgtaag cgagttttac ttcgcgcaaa cgcacccatc
     4141 cgccatcata aacagcttgt gaagatgcgc cgaaatcgcc ctggtagctt tgccaataat
     4201 cagcaggtga aatagcgatg tcattttgtg aatatgtgcc atcagcattt tcaattacac
     4261 catcgattac ataagtttca gtacgatcgc ctgaagcttc gctgataccg atacggttca
     4321 tacgagcaat agttccgcac cagatgtcac cacctttgcg aatatcaagc aatccactta
     4381 cagtgaatcc tttgtaagta agtgcagtgc tgatgttcat caaccaatcc ggatatggat
     4441 cgccgataac accactcact gtatcgaaaa ttggtaagcc tgatgatgga tcgatcaata
     4501 aatcaccatc ttcattatat gcccactgtg aaccgtaaag taaaccataa ggctgaccaa
     4561 cgatcacata agcacccgga tcaccgaaac ctgcttcaat ctcaatttca tcaacaccat
     4621 cagcaagttg tgttacttcg ttttcgtttt tgctgaagtt accaccaaca ttccatgtca
     4681 gatttttctt cttaatgata tctgcattca atgtcaattc aataccctta tttgtcatcg
     4741 cacctgcatt gtcatataca taagagaaac ctgatgtaga tgcaatcggt ttggtgagaa
     4801 ggatatcaga tgattcctgg atataataaa cggcatcgag gtcgatacgt cctttccaga
     4861 atttcaattc gagaccaaat tcagttcctg tcaaacgctc agggctaaga tcaggattac
     4921 ccaatgtatt caattgtgag taaccgaaac ctgattgtcc gaggtaaggg aaactataac
     4981 catctgtgaa accatcggta tacagtggtg gtatgaagta agtgtcagca ctgtaaggtt
     5041 gaggttcgat acctgcttgt gcataagcaa aacgcaattt accgaatgac cagatattgt
     5101 ttgtagtcag gatatcgctg aatacaaatg caacgctggc tgatgggaag aatgctgaac
     5161 tttgattagg tccgtaagta gatgaaaatt catcacgacc tgtcaaagtc aagaataata
     5221 attcgcgata atcaaactcg agatcgccaa agaaggctgc gttacgttgt ttagttgtat
     5281 actgtgaaga atacaagtcg gcagcattcg acaagttata aaaatcagga atggtaaggt
     5341 cgcgaccgcg tgaatattga ttgttgatat caatatcagt gaggttatta cctacagtta
     5401 atgtaattcc taagtcttca tttacttgat gtgtagctgt tgcaataagg tcactgtata
     5461 tctgacgata gcgaatagta ttttcttcga tttgtccacc cggcgcattt gcagggtcgt
     5521 tagaaccgat agagaaaatc tgtttacgca aatcagtata gttatcagct ccaacgcgat
     5581 aagtaacatt taaccaggtc caaggagtgt aagttgctga aacattgctc aacaaacgat
     5641 ttacattatc agtgtacggg ttattattta cagtccagta tggattatca tatgctgaga
     5701 aatagttgcg gttgtcgcct gaaggatatt cataaccggc tccaagatcg tagcttgttg
     5761 gtgttctaag taatgataac attacaccag aaaggttact accattttga gcgcgagtac
     5821 caccactggt gatatagttg gcatcaacgg caactttcag tttatcataa attcgcatgt
     5881 ctgaattaac gcgaactgaa gttctgtcga attgtgtatt tggaacaata cctttatctt
     5941 gtgtattacc aatagaaaga cgcagagatc cgcgatcagt tccaccggaa acactcaagt
     6001 tattattata ggtcatacca ttttggaaga attctccggg gttgtcggtt ggcgtgatgc
     6061 caatatttga catcagtggt ccccatgaat tggcggtacc tgcagaacca tcgtcatctg
     6121 ttccccaaag gaagtcagga cccggatcag aagtaatata attaccttca ggatcaacgt
     6181 ttccatcagc atctgtaaaa cctccacctg aaccttgagc atacaattgt tgctgagcag
     6241 gtattttatt tacctggctc acttcaagtg aagatgagaa attaatgtgg actgctttat
     6301 cacctgcttt attaccacgt tttgttgtga taatgatagc acccgaacct gcgcgtgaac
     6361 cataaagcgc agcggctgca ggtcctttta ggatgttgat gctttcaaca tcatcagggt
     6421 tgatgtcaat ggcacggttt gagttactta caccattcaa tgttgggttg aaaggatagt
     6481 cgcccgcaac agtactagta gtagagttgt cgattggcac cccgtcaatt acaataagtg
     6541 gttgattttc gttggtaaaa gtactcggac cgcgaatgat gatcttagca gatgcaccgg
     6601 gagttcctgc agaaccaacc acctggatac ctgcagcttt cgctgaaagt gcttccacaa
     6661 ggttttcctc accggaactt gcgaggcggt cgccacccac ttcctgtgta gataccccta
     6721 acgaaagttt ttctttcggg atacccaggg cggttacaac caccgtttcc agacctaaca
     6781 cgtcttcgtc catcgcaatg ttcaccacat ttttactgcc gatggattcc tcgagcgttt
     6841 taaagccaag aaatgtgaat actaaaacat ctttgtcttg cggaacaaca agcacgtact
     6901 taccatccag gtctgtaact gtgcccaccg ttgtgccttt taccatgacg gttgcgcctg
     6961 gcagaggctg attgtcggtt gccgacgtta ctgttccggt aatggttttg ccctgcccga
     7021 aggccacaga taccatacca aacattaaca gcagcaccga ggcagctagt tttctcatag
     7081 atttggtttt gaatgaaata ataggtttaa tcttttaact gaccctgtaa agtctaatgt
     7141 taaaatactt taaacaacaa acttaaacgt ctttctgatt ttttagcaat tttcaggtaa
     7201 aaaaaagccc cgaaagtgaa tacaccgggg acttattaac aaattttcct ctttgttact
     7261 tgtgggtatc gtagtaaccg aacacttttt gaacgatttc tgaggctctg gcggccggat
     7321 cctggcgtat tttaagctcc tcctgttcta tcatatagaa tagcccatcc aacgcttttt
     7381 gagtcacata atctgataaa ttgggattca tgtcctctac caatggtatt ttattataat
     7441 aattaacggc atcactccaa tattttgttg cacctacttc actgagcgat ttgtcaataa
     7501 cgggtttgaa ttttgctgtt aattgtgctg ttgttgtttt tttcaaatac tcagtggcag
     7561 catcgtcggc acccattaaa atattcatgg catcgctaaa tgtcatagat cgaatactgt
     7621 taaccaaaat cggcttagct tcagcagcag catcctcagc ggcgtgattt acagaattga
     7681 tcacatcatc acacatttta ttcaatccaa gatcgcgcag cgtggtttct attttctgcg
     7741 cttccggagg aaatggaatt ttcacagatg gatttttcag gtaaccatcc acttttgcca
     7801 gtgtagctga accattggaa gccccttgca ccaaggcttc tatcaatcca ttagaggctt
     7861 cctgatcggt aatcgtggtg ctcataactg tatttaaaac atcctccatt acctgaggat
     7921 cgcaagtggc ccctgtaaat aaatacatgg tgattgcggc taagcagaaa tttctaaaca
     7981 tgacaattgc attttgatgc caaattaaat ctttgtttga aatgttgggc atgcatcgca
     8041 caattatcag tataatttgt tgttatggtc agtaattccc tttgacaact tataccttta
     8101 ctacaaaata caaatatgcg ggctgcactc atttccattg gcgacgaaat tcttaatggc
     8161 acgacaataa ataccaatgc aagttggatc tcaacagtac tgcaaccgtt gggtgtagat
     8221 attcatgagg tgctggctat ttctgatcat gcagatcata tactcggcac gctgcacgcc
     8281 tatgttggcg aaatggaaat tatcatcatt acaggaggcc tgggcccaac caaagatgac
     8341 atcacgaaaa aaacgctctg ccgctatttc gatagcaccc tcgaattcca tcaagacatt
     8401 tacgacaggt taaaagctgc cttcgccaag cgaaacattc catttacgga aaacaatcgc
     8461 gatcaggcca tgtatccgca caactgcgag gtgctgcata atgctctagg aaccgctcag
     8521 ggcatgcttt tcaagcgaaa tggcagcgtt attgtaagtt tacccggggt cccttttgag
     8581 atgaaaggtc tcatgaccga ccaggtatta cctcgtttaa gggaagaatt gcaattccct
     8641 accctgctta accaatattt tatgacaagt ggtatcagcg aaagttttct ggctcgtcaa
     8701 ctcgagccac tggaggctat gttgcccggt catattggat tagcctacct cccatcgccc
     8761 ggagttgtaa agttgcgatt gacagcaaga ggctccgatg ctgcaacact caaggcagaa
     8821 ttagagatgt taagcaatga aatgcgacga atactgggat ccggtattta tgctgaggaa
     8881 cctatctcga tagaggaaca tttaggaaaa ttgcttgtcg agggcaaggc aactctagga
     8941 tgcgctgaaa gttgcacagg aggcaagatt gcgcataaga tcactgctat tccggggagt
     9001 tccaattggt ttaaaggcgg ggtgattgca tacgataatc aattgaaaat caatattttg
     9061 caggttaatg aatccatcat ttcccaattt ggtgccgtaa gtgaagaaac ggtatctgct
     9121 atgctggatg gcgctttaac cgttttgggg accgatttcg ctattgcaac gagtggaatt
     9181 gccggcccga caggcggaac tgccgataaa ccggttggta ccgtatgggt tggagtagcc
     9241 ggaggaggca aaaaagtggt ccacaaatat tttttcaaca agaatcgaga gatcaatatt
     9301 gaatatgctt gcatgtttgc gctgcatgag atgcgcttac tgttgactga aatccttgtt
     9361 aaaccatctt aaagggctta tttttgcgaa tactgttaac ataggcatgg tgtgtctgtg
     9421 ggttacgact agtgtactat ttacaattcg aattaaacat ggcgcagaaa gaactgatca
     9481 tgcccaagat gggcgagagt attatggaag caaccattct gaagtggttg aagaaagaag
     9541 gcgataccgt tagcgttgat gaagcggttt tggaaattgc tacagacaaa gtagacagcg
     9601 aggttccttc accctttgca ggtactttga ccaagatatt atttaaggaa ggggatgttg
     9661 ttcagatagg taaggcgatt gcaatacttg atggcgacgg atcagttaca acaaaaactg
     9721 ctgcaccgga gccggcagta aagactgaag tagttgtaga aacacctaag gtagttgcgc
     9781 cggtagtgca aactgaagtt cctgtagttg ccggtgacag gttttatagt ccactggtat
     9841 taaatattgc acgcgaggaa ggcatttcaa tggccgaatt agagcgtatt ccgggaacag
     9901 gaaaagataa tcgcgttact aaaggcgata ttctcaatta cgtgcagaat agaggtgccg
     9961 gcacaacaca ggcaccggta gaacaacaag cacctgttac acaaactaaa actgaagaac
    10021 cggtaattgt aaaaactgct tcagtaagtg gtaatgtaga gatccgcgaa atggaccgta
    10081 tgcgcaaact tattgccgat catatggtta tgagtaaacg cgtttcgcca cacgttacat
    10141 cgtatgttga agtagatatg acgccaattg ttgaatggcg taatcgtatc aaaggaaaat
    10201 ttgaacaacg cgaaggacag aaaataacct atacgccaat atttattgag gcaattgcaa
    10261 aagcaattaa agattttccg ggtattaata tttctgttga cggcaataat attatttaca
    10321 aaaaagacat caatatcgga atggcagctg cattgccaag tggtaatctt atcgttccgg
    10381 taattaaaaa tgccgatcgc atgaatatgg ttggattaac catggtcgtg aacgatcttg
    10441 caaacagagc gcgtgccaat aaattaaagc cggaagaaat tcaggatggc acattcacgc
    10501 ttaccaacgt tgggtcattt ggcagcctga tgggaacacc catcatcaat caaccacaag
    10561 tagctattat ggcagttggt gctatcaaaa aacgtcctgt agtgttggaa accacacaag
    10621 gtgatgtaat tgccatcaga catatgatgt ttatgagtat gagttacgat caccgagttg
    10681 ttgacggtat gttgggcgga tcattcctga aacgcgtagc tgattatctg gaaggatttg
    10741 atactgaacg cgaattataa tttattggtt tttcatatca ataaaccaca cacatgtaca
    10801 tagaacaact atatacgaat tgtttagctg aagcggccta ttacattgaa agtaatggcg
    10861 aggcagctat cattgatcca cttcgcgata cggctccata tattgaaaaa gctgcttctc
    10921 gcggggcaaa aatcaaatat gtttttgaaa cacattttca cgcagatttt gtttcaggcc
    10981 atattgatct agctcgtcaa accggagctc aaattgttta cggaccagag gcaaatacat
    11041 cctatgatgt agtaaatgct gcagatggtg aaatatttca tgtaggctca cttcaaattc
    11101 gcgcattaca tacgccgggt cacacattgg aatctacttg ttatttgttg atagatgcca
    11161 atggaaaaga ccactgcatt tttaccggtg atacattatt tgttggcgat gttggtcgtc
    11221 cggatctttt ggatggtatt atgaccaaag aagaactggc atccaaaatg tatgactcgc
    11281 tgaataataa aataaagccg ttagcagatg atgttgtagt atatcctgct catggccctg
    11341 gaagtgcctg tggtaaaagc atggggaaag aaacattttc caccattggc attcagaagc
    11401 aaactaatta cgcactgcag ccaatgacgc gtgatgaatt tatcgccgcc gttaccgatg
    11461 gtttatcgca accaccaaaa tattttttcg aagatgcacg cattaacaag cagggctatg
    11521 aagatgtaga aatggtaatg gcaaaaaatg caaaaccact ttcaataaca gcatttgaaa
    11581 ccgcggtgga atcaggtgct ttaatattag atacccgaat tgccgatatt tttgaaaaag
    11641 catttattcc taatgcatta aatatcggcc tcaacggaat gtttgctgtt tgggtaggca
    11701 ctttggttga tatcgatcaa ccgcttgttt tggttactga accaggaaaa gaatcggagt
    11761 cagtgttgcg acttgcacgc gttggatatg aaaatgtatt aggttttctt gaaggtggaa
    11821 ttgatgcctg gatacaatca ggcagaacta ttgaaacggt aaccaccatc gaccctggca
    11881 gcattaaacc atatatgggt aatgacgatt atattgtgct ggatgtgcgc aaaccatctg
    11941 aattcgaaaa cgaacatatc agtggtgcca ttaatataca attaagtgag attggtgctc
    12001 gtttacatga attgcaacct aacaaacaat atattatgca ttgcgcggca ggttacagaa
    12061 gtatgatggc agcatcaatt ttaaaagcga atggtttaaa caattttgta aatgtgcgaa
    12121 acggatggac aggaatcaag aaaactgatt taccaactga gtcatccgtg cctgaactaa
    12181 attaatgttg ctgcgaaaag caaaatgcga aaactgatat tgatcatagc tgttattatt
    12241 tcgttcacct cctgcgataa tttcaaggag agtgaagcta ttgaacatat cacacgcaca
    12301 tggacggttt acaaatatga agttgacgga tacgatcaaa caccattatt tgtagccaca
    12361 tatggcacct acactttagt ttgtgagaaa gatcaggatt atactgaaac atataccgta
    12421 ggtgcaacac ctgttactat taccggtatt tacattttca gcgatggcga taatattctg
    12481 acattgtcag atgaatttca atcgcgtgtg tttaatgttt taacgcttac gaataatgaa
    12541 atgactttag aagatcgcaa tgcagatact aatcaggttt attaccttga agcagtgatc
    12601 tgacaaaata caattaacaa aaaaggcgat gatcactcat cgcctttttt gttgttattt
    12661 accctggcgt tttacatcgc ctttatcttc aaatgatttg aaaactgcag cagggtcata
    12721 ttttttaact gcctttcctt tgcgtatgat tttgatatga ttgatcttat cgccttgttg
    12781 aatagcgttc accacatcct ggccttcaac cacatgtccg aaaacagcat gtttattgtc
    12841 gagccatggc gtttctttgt gcgtaataaa gaactgactt ccgttggtga atttgccggc
    12901 atttgccatt gataaaattc ccggacctgt atgacgaagg tcgctatgga attcatcttt
    12961 gaaagcataa cccggtccgc cggagccatt gcctgaagga tcaccaccct gtatcatgaa
    13021 atcagcaatg acacgatgga aggttagtcc atcgtagtaa ggtgtgccca gtggtttagc
    13081 ggtattttca atttttcctt ctgccagtcc cacaaagttg gcaactgtga gaggcgcttt
    13141 ctggaattcc agaaatagca ggatattgcc ttttgttgtt tgcacttcgg catacaatcc
    13201 atcgggaaga tgcttgtatt ttttacgatc actttgaccg aatactgccg aatgcatcag
    13261 cacggctagt aataacaata cacttgtttt tttcatatca cgattattga tttattgatt
    13321 tgtatttgct tcaggcatgg cataaaattt tggcaacacc tcttcaaagg tttttatggt
    13381 atcctcaagc gactgctttg atgagccgcc ggaagcattc atatgtccac caccattaaa
    13441 gaaatttcgt gccaaaatct caacattcac atcgccttta gacctcagcg acattttaac
    13501 ttttcctttg tcttcggtaa aaagcaccgc tatatctata tccttcatcg ccaaaggaag
    13561 attaacaatt ccctccttat cacctatgct caatttatat tgttcagctt ctgccatggt
    13621 tatactcatg tatgctgctc tatatgcagg tatgatctgc aatttttgcg ataagagata
    13681 tccgataaag cgtattctgt tttctccata ttgattatag aggtcgttat gaatacgatc
    13741 aactttaatg ccatattcca tcaacttccc tgcaatagca tggacgtgtg cggttgtgtt
    13801 gctatactga aatataccgg tatcgagtgc aattcctgca taaatgcacg cagccatatc
    13861 ctgatccaac agatggatat cacctgccat ctccataaat gtatatacca attcagcagt
    13921 tgaactggca tggtcgttcc acaatctgta atccgtgaaa ttatccggct ccctgtgatg
    13981 gtctatcatc acgcttacat atggtcttgt cagtttcatt acatcttccc ccaaagcttc
    14041 aatacgtttg atggcattaa agtcgagaaa gaatatagca tctgactgtg ttagaatagc
    14101 aaagcaggtc ttgcgattgc gctgatggat gtatacttgt ttactacccg gcatccaatc
    14161 gaggaagtcg ggaaaatcgt caggtgcgat tacattggcg gtatgacctt tcttctcgag
    14221 gtacattttc atggcaagtg ccgagcctat tgcatcgcca tcgggacgca catgagtaag
    14281 caatgtaatc cttcttggtt gcttgagcca gttaaagaga ttggtcagtt ccatagttaa
    14341 ttcgggtact aaggtcacca ttttttattt tttattaaaa tggacgttac gaatacgtat
    14401 ctttgcgccc gaaaataata tgttatggca ggaaatatca cattaaccat gatcaaaccc
    14461 gatgctgtta aaaagaacta catcggtccc atcttaaaaa tgatcaacga agcgggcttc
    14521 cgcattgttg caatgaaata cactaagctg agcaaagaga aagccggtga attctatgct
    14581 gttcataaag aacgttcgtt ctatggtgaa ctggtagatt tcatgagctc tggtccgatc
    14641 gttgctgcaa ttcttgaaaa agaaaatgca gttgaagatt tccgcaaact gattggtgca
    14701 actgatcctt caaaagctga tgaaggcacc attcgtaaat tatatgctgc cagcattggt
    14761 gaaaatgcag tgcatggttc cgacagtgat gagaatgcag ctatcgaagg cgattttcat
    14821 tttagcaaat tggaacgctt ctgaaaataa atcacgaaaa gaaaagaaga tccggccatg
    14881 ttgccggatt tttttttgct gcaacgaatt caaattttac cgtgcaataa atacttcact
    14941 ttttgattgt atgatttatt gatgatgttt tacaatcaaa aaaaaacccg ttacggattg
    15001 tgtccggaac gggttaacca aaacaggttt ccaatggcaa aaatgttatc tggaaacctc
    15061 tatctttcgt atcgtagttt ctgttgaaga acttaactga agcaaatata atccgtttga
    15121 aagtcccctc aaatcaaatc tgaaaatatt ttttcccaca gtacataact cgctggttgt
    15181 cagcacttta cgtccttcta aatccagaag atcgaaggta atgtctgatg ctgacggcat
    15241 gtcaatttca acggtcattt cgtcagtcgc agggttaggg gaaacggtca tttcagccat
    15301 ggcaagtgca gttgccgaaa ccccgaaatt caccgtaacc acgtaggttt taggcgtata
    15361 atctaccggt gaaaagcatg tataattgac tggagaagtg aagttttgag ctacccctcc
    15421 taccgggtca acatactgtc ctgtgtagtc tatagtgggc actaaggctg ttacatcagt
    15481 tcctaccggt acggtcaggg tgatagtacc tgctgcctga tcgatagctc cgctagctac
    15541 cggagtggtg aaattgaaaa ggacgatact gtttggatag gtggtgtggt atccatcgat
    15601 atcattttta tcgcgaggaa ggagtttcca gttactaaaa ctgtaataca tcaagccctg
    15661 gatatagctg agcgactgcc ccatagcaag gctgtctgtg ccaaattcga aaggcaactc
    15721 ataagagtag tcatcgcagc gcatgccaac ttcaggggta ttgctgaggt tcaccctcca
    15781 ctcgccaaaa gcaccgccgg tagcggcatc tgcgttgttg gaagtaacaa aagcattgtc
    15841 gaaacgcac
//
LOCUS       DAOQHY010000074        15527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1816_length_15527_cov_5.754524, whole genome shotgun sequence.
ACCESSION   DAOQHY010000074 DAOQHY010000000
VERSION     DAOQHY010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15527
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1816_length_15527_cov_5.754524"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PLL28_07645"
     CDS             complement(<1..391)
                     /locus_tag="PLL28_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69235.1"
                     /translation="MAVAKFFPQIILGTAVSVTVMSGILRNDPQMVADHAASDTYFFS
                     PAAPIDTPPSLHYPFPDDDGSNPFQNDENNFDLEPTQQPTIIYDPETNEYLIQNEIGG
                     DFIENPQYITFEDYVNAQEDQAIDDYWK"
     gene            complement(402..989)
                     /gene="ruvA"
                     /locus_tag="PLL28_07650"
     CDS             complement(402..989)
                     /gene="ruvA"
                     /locus_tag="PLL28_07650"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNF69236.1"
                     /translation="MIAYLSGQFTVKNPAYMVVECNGVGYQVHISLATYSKIQGMEKG
                     RIHTYMVVREDAQLLFGFADETEKNMFVNLLSVQGVGPNTARMILSSMNVDELEQAIL
                     SENDPLIQKIKGVGPKTAKRIVIDLKDKVVKGSTGTENSFGASHNTIKEEALIALAML
                     GFSRQQGEKAIAAALKNSPNPVDVENLIKLALKNL"
     gene            complement(1255..3594)
                     /locus_tag="PLL28_07655"
     CDS             complement(1255..3594)
                     /locus_tag="PLL28_07655"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNF69237.1"
                     /translation="MIYTCSVITTFVGSTAFSMPEKLRKKDALDYHSSGRPGKIEVIP
                     TKSTKTQRDLSLAYSPGVADPCLEIAANQEDVYKYTAKGNLVGVISNGTAVLGLGDIG
                     PEASKPVMEGKGLLFKIYADIDAFDIELNCRDVDEFVRIVKAMEPTFGGVNLEDIKAP
                     ECFEIEKRLKAEMRIPVMHDDQHGTAIIAGAALLNAIEILGKDLSTIKVVYSGAGASA
                     ISCARLFVQLGVTRENITMLDSQGVINAQRDGLDEYKSQFISNTTATTLAEAINGADV
                     FVGLSKGNILTPEMLLTMAKDPVVFALANPDPEIAYDLAVATREDVIVATGRSDHPNQ
                     VNNVLGFPYIFRGALDVQATEINEAMKVAAIRALAALAKEPVPEFVNMAYGEKNLMFG
                     RGYIIPKPVDPRLITRVAPAVAKAAIKSGVARRTITDWEEYEHELSNRLGLEQQLIKA
                     ITSKAKQNPKRVVFAEADNYKILKAAQIVREEGIAKPILLGPVEKIKRIIEENQLDLR
                     NVPIIDTRSTDQEETRARYGEIFFNKRMRRGVNLYEARKMMRDRTYFGAAMVETGDAD
                     ALISGLTRQYPETIKPALQIIGREDGSKKVAGMHIVMTKHGPVFFADTTATFNPTAEE
                     LVEITALTAKAVRQFNIQPKIALVSYSNFGSVENDETLVLRNAVALLKERFPGMIVEG
                     EMQVNIAFENELMKENYPFSALADKSVNTLIFPNLHAANIGYQLMQSLGQSEVIGPIL
                     LGMKRSVHILQLGSSIREIVNMVAIAVTDAQVKSKRKAL"
     gene            4152..5087
                     /locus_tag="PLL28_07660"
     CDS             4152..5087
                     /locus_tag="PLL28_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69238.1"
                     /translation="MATVTFKKIVYLHSGTGVRHHHYSSSEGTISGPVTSNYPGTTDT
                     TSGTYSADGAAQEIYNGQLVNFAFMAVKGSADGNQLFTSAGDQSIRVGSNNVTVLVVY
                     APPGGIGVNGGPGVWVDAFNVDTCAFSDDLHFIQVFTPPTPPDNLDAAKTDFANMEGE
                     ISTVTAEHMRASTTVDGAIPFLEWKQVTSNDSPVGSRDVDLAQGQSGQIWFAFYKTPP
                     APPAPSIPHFNDRLGWIYLSPGVLVGGGGFGIGPDGKPHPIDPDGPLMQQLLSTFAMI
                     SVSANMHEKLQMEARRIASDNLRMIADSVQNKRLK"
     gene            complement(5150..7222)
                     /gene="scpA"
                     /locus_tag="PLL28_07665"
     CDS             complement(5150..7222)
                     /gene="scpA"
                     /locus_tag="PLL28_07665"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF006944.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HNF69239.1"
                     /translation="MSKRSLRNIPYHHYQLPSVQVQPDAGFDVVKKSLPGSAPFLRGP
                     YSTMYVMQPWTIRQYAGFSTAEASNAFYRKNLAAGQKGLSVAFDLATHRGYDSDNPRV
                     FGDVGMAGVAIDTVEDMKILFDQIPLDQMSVSMTMNGAVIPIMAFYIIAAEEQGVTPQ
                     QLAGTIQNDILKEFMVRNTYIYPPVSSMRIVADIFDYCAKNMPKFNSISISGYHMQEA
                     GATPEIELAYTLADGVEYVRTGIAAGMQVDDFAPRLSFFFGIGMDHFMEVAKLRAARV
                     LWCRLMEQFAPKNNKSLMLRTHCQTSGWSLTEQQPFNNVARTCIEALAAALGGTQSLH
                     TNSLDEAIALPTDFSARIARNTQIYLQKETGICDTVDPWGGSNIVEALTDKLIHDAWK
                     LIQEVENLGGMQKAIEAGIPKMRIEEAAAKKQGRIDSGTDVIVGLNAFKTEEENNIDI
                     LEVDNSAVRNAQIERLHEVRNSRDNNAVKQALAKIEDAAKTGNGNLLALAVDAARSRA
                     TLGEISDAMEAAFGRYTAETRTIAGVYQKEVMQNETFKQARNLSDAFAGKAGRRPRIL
                     IAKLGQDGHDRGAKVIATAFADIGFDVDISPLFLTPEEAVKQAIENDVHIIGISSLAA
                     GHKTLVPRVIDLLKEQGREDMLVVVGGVIPQQDYAFLEEAGVAGIFGPGTVIADAAIT
                     LLQRLMTE"
     gene            complement(7225..8427)
                     /locus_tag="PLL28_07670"
     CDS             complement(7225..8427)
                     /locus_tag="PLL28_07670"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNF69240.1"
                     /translation="MDELINNDIEKAPWLQQVTKDLKGADFREKLVHIKEGIEIMPLY
                     TASDLPEFEPDANADFLADMDDDFAAEVGNQHQQHLTWNITEDWDVSASTNMHELVQQ
                     ARQRGVIDLRLHAEVDSDYDAIFDGIRKMGSPANLHFDATGPINEHTAGSWRERIGFL
                     GDRDRLIQSIQFDPISYWEKNGYPASTNAQFDRLADAILRINGHLQDCRLIKVDTTDL
                     ADAGASSIDQLVKALHRTAVYFEEMEKRNLSLFEITQLFTFRFGVGTNYFFEMAKLRA
                     FKILWNNLVKAYDPELDFIPTPYIHAVVSTHSFTQEDKHSNLLRSTTAAMSALLGGVD
                     ALSIPTFDLQQEAQSVRLAINIQNLLRYESYMDRYRDAANGSYYIESLTRQFGEKAWS
                     IFIETHNA"
     gene            8523..9446
                     /locus_tag="PLL28_07675"
     CDS             8523..9446
                     /locus_tag="PLL28_07675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69241.1"
                     /translation="MKTVYRKLEAYSAAAIALMAVKTAEAQVHYADVDPDIVLDSPDA
                     FALIDLNGDGLADIKFANQSFYLLLWTFYSEEKPYLFQYINASAYAELFAYNENSVAY
                     TGSEGGLSGGVWHVAKAIEEGEVVNAELDFEEWGVPMAEKIYSCTLTAGFGATSCVFS
                     DSVGKWQPELANRFIGLHFTNITDIGYFGWIRCSIANDGRKLTIHDYAYNATPEQAII
                     AGDTLAVGISEATLQGTQMYTDGNNVLHAKTTSSGNTRLQLFDISGREIVFLQNNIGE
                     FNYDLTALPAGPYIVRLTQGASSSTIKIIRK"
     gene            9443..12211
                     /locus_tag="PLL28_07680"
     CDS             9443..12211
                     /locus_tag="PLL28_07680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69242.1"
                     /translation="MNAWIKPAILTMCFICSNKLQAQWSTVDSTFSDDGIAIISAGAG
                     NDRCYTSAVQSDGKLIIVGNSIYESGGYFISISRLFPDGTPDPAFGTGGIAIQYVNGD
                     GYGSPLMGSVEKVLIQPDEKIVLVGQVVNTAVDEFNYDYFIMRLMPDGSRDATFGTDG
                     FSMYGYFDEWGYFQRDYARSAALLPDGKIVIVGDTGPYGLSYASIMRILPNGLVDPTF
                     GTDGSYFINVSDDSYGRGIAVLGDGSMVMSGYFFDGQYVFFAKWNADGLMDETFGIAG
                     IQIDSTIAGFNLKRMAITPDEKFILMGEKNSTVMINRYTSEGIADTTFAYFELPHSGT
                     IYYAEDLFIDEENHTYFCYHASNPKDYFLGKLTDVGGLDYSFNASGTAQFDLTTGISE
                     CQSVVMSGETLYASGYADFQNDYDIATIAVTTEGEIDNGFATDGVWRANVGRSADYGH
                     AIALTPDNKILIAGNNGFVLVARVNETGVLDSTFGNNGVASHALYYGISYPTVTSVLS
                     LPNGRIVVLAYDDDLVVSRLYPDGSLDPTFNGGYTNLTVGDEELYYTYDLAYQEDEKV
                     LFSGIYYGYGFAYEEAVFLIGRLNEDGTFDSTFNGDGLNYDANFSESLNIARAIAVMP
                     DDRIVAGGYYVNDNEKQYPRLVRYMPDGSRDNSFSGDGAFGATINGEEGRILDLKVDI
                     ENRIVGAGYVGAVENDTVQIMVMRVTADGELDTTFADGGYFIYDTPLEASARSIAVDD
                     SNRIVVSGYAYIEIGNAAFFTLRLLSDGTPDPTFGINGMLFTDLTPEDDIAYSMQIQD
                     DGKIILGGTSSENLNTNFALVRLLPENGFTEPIDSDTTYGSFLVFPNPIHGAFVLQYQ
                     LLDAQQVSIGLFDAAGNNVAQLMTNEARDAGDHQEALQLPETLARGYYFLRISCSDLV
                     TMVQIVVM"
     gene            12285..13073
                     /locus_tag="PLL28_07685"
     CDS             12285..13073
                     /locus_tag="PLL28_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNF69243.1"
                     /translation="MQLENLAIDIRENILYITINRPKNLNALNIKTIVELDEVFEEYM
                     YNDDVKGVIITGSGEKSFVAGADISEFADFTPEQGRTMSENGHDTFDKIEMYNKPVIA
                     AVNGYALGGGCELAMACHIRVASENAIFGQPEVNLGIIPGYGGTQRLVELIGKGKALE
                     LLMTGDSINAALAKELGLVNYVVSPEELMPKCEEIMKKIIGKGPVAVSKVVQCVNAHF
                     RGGGYVGDSYQTEITQFGQCMETEDFVEGTTAFLNKRKPEFKGK"
     gene            13219..13797
                     /locus_tag="PLL28_07690"
     CDS             13219..13797
                     /locus_tag="PLL28_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69244.1"
                     /translation="MRNYDWPQVYALVQAMTRNEKGYFRKLHEGFGGDSQRQYMQLYA
                     LLEKQKTLDLGAIKKAFAGQGINLTSTRNHLMQQLLRSLRNYSVGKSDLMQLRELLDY
                     AALLEKKGLHQACMELTKEGIDLAQKNNHPWYVILLLNIQRSIIDLMPENERDALAAA
                     IYQKTVEANKSLQTYRWDVGDKHRHKGKGNQN"
     gene            13901..14476
                     /locus_tag="PLL28_07695"
     CDS             13901..14476
                     /locus_tag="PLL28_07695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HNF69245.1"
                     /translation="MKKRISLFLLTAILGFSHFSNAQVNAVKPFKTVIIGNQEWMSVN
                     WDYKTPKSFFYNNDSTIDSKYGRLYFYSNAVAAAPPGWHLPTLDEWWELMNYLGGINK
                     AAPLMMASDTIGLHLVSGGYKSANISADNSDLYGFIDQYAFYWTATPDAEQTAYAIRI
                     TPSTGEIEVNSFRRANGFSVRYVKDKEVKED"
     gene            complement(14497..15132)
                     /locus_tag="PLL28_07700"
     CDS             complement(14497..15132)
                     /locus_tag="PLL28_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69246.1"
                     /translation="MKKILTVLFLFTLSNYAIGQDQIDTYIKDAQTYIAAKDYKNAQL
                     SLQDAINELNNLIGQQIAESLPAEINGLKADGDATTNSAAMGMMGGGIMISKSYSNPT
                     NKNNDAEINIVANSPMIATMGMYMNNPAMLGEGYKSVKVGTQRAILKSEMEDRFVEDG
                     KPEIQIRSTEIQIPLGQTLITLQLSGFATEADELAFASKFDLAKLKTALGE"
     gene            complement(15235..>15527)
                     /locus_tag="PLL28_07705"
     CDS             complement(15235..>15527)
                     /locus_tag="PLL28_07705"
                     /inference="COORDINATES: protein motif:HMM:NF025846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNF69247.1"
                     /translation="ETLEIFQTGKSIGEIAAIRELSPNTIEGHLTECLAEGLISVEKF
                     ISPDKMKRIIQVRQENPDAKLTILKEALGDAYSYFEIRVTLTAMKRKEKESS"
BASE COUNT         4234 a         3208 c         3413 g         4672 t
ORIGIN      
        1 ctttccagta atcatcaatt gcctgatctt cctgagcatt tacgtaatct tcgaaggtga
       61 tatactgtgg gttttcgata aagtcgccac caatttcatt ctgaatcagg tattcgttgg
      121 tttcaggatc gtaaattatg gtaggttgct gagtcggctc gaggtcgaaa ttgttctcgt
      181 cattttggaa tggattagac ccgtcgtcgt cagggaaagg gtagtgcagc gatggaggag
      241 tatcgattgg tgctgccgga ctgaaaaaat acgtatcaga ggctgcgtga tccgcaacca
      301 tttgaggatc atttcgcagt atacccgaca tcacggttac actaactgct gtacctaaaa
      361 ttatctgggg aaaaaatttc gcaactgcca aaacctcttg tttacaagtt cttcaaagct
      421 aatttaatca ggttctccac atcaaccggg ttcggactgt tttttagggc tgcggcgatg
      481 gctttttcac cctgctgcct actaaatccg agcatcgcca aggcgattaa cgcttcttct
      541 ttgatggtat tgtgtgatgc accaaaagaa ttttcagttc cggtcgatcc cttcaccacc
      601 ttatctttca ggtcaataac aatgcgtttt gcggttttag gtcctacgcc tttgattttc
      661 tggatcagcg ggtcgttttc cgacaatatt gcctgctcca gttcatctac attcatgctt
      721 gaaagtatca tcctggctgt gtttgggcct acgccttgca cagaaaggag gtttacaaac
      781 atgttttttt cggtttcatc tgcaaagcca aagagcagtt gggcatcttc acgcacaacc
      841 atataggtgt ggatccgtcc cttttccatg ccctgtatct tcgaataagt agctaaactg
      901 atatgcacct gataacccac tccgttgcat tccacaacca tgtaggcagg gtttttaacg
      961 gtgaattgtc cggataaata tgcaatcatg tcgcagtgga tgagttttca gtatggatat
     1021 ttctgcacca caactacggc aaatgtttga aacatagcca tttagtacat gcagaggggg
     1081 caaatttcgt ggaaattata ggaatttgaa agccttatgg aaaataaggt gcaacctaat
     1141 ttatggtttg acttttatat gtttcaaacg ccttcaagac aaacgcataa ttccgtaaaa
     1201 ccaattgccg gaattgatcc gaaatgctca gtgtaaattg cctgaaaccc ttgattacag
     1261 tgcctttcgc ttcgatttca cttgcgcatc ggtaacggca atggccacca tattcacaat
     1321 ttccctgata gagcttccca attgcaggat atgtactgat cgcttcatac ctaacaaaat
     1381 aggaccgatt acctccgatt gtccaagaga ttgcatcaat tggtagccga tattagcggc
     1441 atggaggttc gggaaaataa gggtgttcac gcttttgtcc gcaagggcac tgaaaggata
     1501 attttccttc atcagctcgt tctcgaaagc gatgttcacc tgcatttcac cttctactat
     1561 catacccgga aaacgctcct tcagtaaggc tacggcattg cgcagcacta aggtttcatc
     1621 gttttcaaca gatccgaagt ttgaatagga aaccagcgct atcttaggct gaatgttgaa
     1681 ttggcgaaca gcttttgcag tcagagcggt tatctcgaca agttcctcgg ccgttgggtt
     1741 gaaggtggca gttgtatcgg caaaaaatac cggaccatgt ttcgtcatca cgatatgcat
     1801 cccggcaact tttttcgagc catcttctct tccaataatc tgaagtgcag gtttaattgt
     1861 ttcaggatac tgacgcgtca atccggagat caaggcatct gcatctcctg tttctaccat
     1921 tgcggcaccg aaatacgtgc gatcgcgcat cattttacgc gcttcgtaaa ggtttacacc
     1981 gcggcgcatg cgcttgttga agaagatttc accatagcgg gcgcgcgttt cttcctgatc
     2041 ggtgcttcgg gtatcaatga taggcacatt gcgcaaatcc agctgatttt cttcgataat
     2101 gcgcttgatt ttttcgacag gaccgagcaa aattggcttc gcgatgccct cttcgcgcac
     2161 gatttgggct gctttcagaa ttttataatt gtcggcttcc gcaaatacta cgcgttttgg
     2221 gttttgtttt gccttcgagg taatggcttt aatgagctgt tgttccaatc ccaatctatt
     2281 cgacaattca tgctcatatt cttcccaatc ggtgatggtt ctgcgggcaa cacctgattt
     2341 tatggcggcc ttagcaacag caggtgcaac acgtgtaatc aatcgcggat ctacaggttt
     2401 tggaatgata taacctctgc cgaacatcag atttttttcg ccataagcca tgttcacaaa
     2461 ttccggaacc ggctccttgg cgagtgctgc caatgccctt atagcggcca ctttcatcgc
     2521 ttcattgatc tcagtcgctt gtacgtcaag tgctcctcga aagatatatg ggaatcccaa
     2581 aacattgttc acctgattag gatgatcgga acgtcctgtt gcaacaataa catcctcgcg
     2641 ggtggctaca gcaagatcat atgctatttc cggatcgggg tttgcaagtg caaatactac
     2701 cggatctttc gccatggtta acagcatttc aggggtaagt atattccctt tcgacaaacc
     2761 aacaaaaaca tcggcaccgt tgatggcttc tgccaatgtg gtggcagttg tattggaaat
     2821 aaactgactc ttatactcat cgagtccatc gcgttgtgca ttgatgacac cctggctgtc
     2881 gagcatggtg atattttcac gcgtaacacc gagttgaaca aataaacgcg cgcaactaat
     2941 tgctgaagcg cctgcgccgg aatacacaac tttgatggtg ctgaggtctt ttccaagtat
     3001 ttctatggca ttcagcaatg cagctccggc gataatagca gttccgtgct gatcgtcgtg
     3061 catcaccgga atgcgcattt ctgccttcaa ccgtttctct atttcgaagc attccggagc
     3121 cttgatatct tccaggttaa caccgccgaa tgttggctcc atagctttga ctatgcgcac
     3181 aaattcatct acatcgcggc aattcaactc aatatcaaag gcgtcgatat cggcataaat
     3241 cttgaacagc aggccttttc cctccattac aggcttagat gcttcaggtc cgatatcgcc
     3301 cagaccaaga acggcagtgc cattgctgat gacaccaacc aggtttccct tggcggtata
     3361 tttatataca tcctcctgat ttgcggcgat ctctaaacaa ggatcagcta cccccggaga
     3421 gtatgccaag gacaggtcgc gctgtgtttt agttgatttg gtggggatga cttcaatctt
     3481 accgggacgg cctgaggagt gataatcgag ggcgtccttc ttgcgtaatt tttctggcat
     3541 tgaaaaggct gttgaaccaa caaaggtagt aataacagaa caagtgtata tcatagacct
     3601 gaaattgcta actcctatgc caatttttag ttgtggcgcg aaagtgtaaa gtcgatgagt
     3661 taataaaaat ttaaaattga aaaagttgct gctgtcaaag tgatatttgt gattaaatgg
     3721 aagtatgtat gcatgctatc atactatagg tctgcaacaa agaatttcac cggataaaaa
     3781 tttcattcac cgaaaaccgt gaataaaaaa gtgctgaaaa aaaatatttt gtgacagcat
     3841 ttattctcag tcttaacatt tgaatttttg caatcaagta taagtaggta tttgaacggt
     3901 tttgcgtgtc aaaacctgtg acatcagtgc attcacggta attggtgaat ttgtatataa
     3961 ggtattgcat gtaattgtaa tgcgaaataa ttttgttttg tcagcagttg tttttttcaa
     4021 aagaagatca ttctcctgca caaataattt cttttgtaat cccataaatt aaacacactg
     4081 attgctttaa gaaaagaagg aaaacgccga aaatcctaaa tagagtaggc atcacttaaa
     4141 aacctttaaa aatggcaaca gtaacattca aaaaaattgt ttatctccac agtggaacag
     4201 gtgtacgtca ccatcattat tcatcttccg agggtaccat ttccgggcct gtcaccagta
     4261 attatccggg aactactgat acaacctccg gaacttacag tgccgatggt gctgcgcagg
     4321 aaatttacaa tggtcaactg gtgaatttcg cttttatggc tgtgaaaggg tctgccgatg
     4381 gcaatcagtt atttaccagt gcaggggatc aaagcattcg cgtaggcagc aacaatgtca
     4441 ctgttctggt agtgtatgcg cctccgggtg gaattggtgt aaatggtggt cccggtgtat
     4501 gggtagatgc atttaatgtt gatacctgtg catttagcga cgatctgcat ttcattcagg
     4561 ttttcacacc tccaacacca ccggataatc tcgacgcggc aaaaacagat tttgccaaca
     4621 tggaaggcga aatttctacc gtaacagcag aacatatgcg cgcatccact actgttgatg
     4681 gtgcaattcc attccttgaa tggaaacaag tgacatcaaa tgattcacct gttggttcga
     4741 gagatgttga tttggcacag ggacaatcgg gacagatttg gtttgctttc tataaaacac
     4801 cacctgctcc tccggcacct tcaattccac actttaatga tcgtttaggt tggatctatt
     4861 tgtcgccggg agtacttgtt ggcggtggtg gatttggaat cggacctgat ggtaaaccgc
     4921 atccgattga tcctgatggc ccattgatgc aacaattact cagcacattt gctatgatct
     4981 cggtgtcggc aaatatgcat gaaaaattgc aaatggaggc cagaagaatc gcttccgata
     5041 atttaagaat gattgcagat tcagtacaga ataaaagact gaaatagtaa ttatcaaccc
     5101 aaaaccccgg agtgaaaact tcggggtttt atttttttgc gcattgaaat tattctgtca
     5161 ttagtctttg taacaaggta atagcagcat cagctatcac cgttcccgga ccgaaaattc
     5221 cggcaacacc ggcttcttcc agaaaggcgt agtcttgttg tggaataaca cctccaacaa
     5281 ctacaagcat atcttcgcgt ccttgttctt ttaaaagatc aataacacgt ggtactaatg
     5341 ttttatgtcc tgcagctaat gatgaaatac ctatgatatg cacatcattt tctatggcct
     5401 gtttaacggc ctcttcgggt gttaaaaata atggtgatat atctacatca aatccaatat
     5461 cagcaaatgc agtagcaatt actttagcac ctctgtcgtg accatcttgt ccgagttttg
     5521 caatcagtat acgtggacgt ctaccggctt ttccggcaaa tgcatcgctt agattgcgcg
     5581 cttgtttaaa ggtttcgttc tgcataactt ctttttgata cacgccggca atggtgcggg
     5641 tttcggcagt atagcgtccg aaggctgctt ccatcgcatc acttatttca cctaatgtag
     5701 cgcggcttct tgcagcatct actgccagtg caagtaaatt gccgttgcct gtttttgcgg
     5761 catcttcaat ttttgcaagt gcttgtttca ccgcattgtt atcgcgcgag ttgcgcactt
     5821 catgtaatct ttcaatttgc gcatttctta ccgccgaatt atctacttca agaatatcga
     5881 tattgttttc ttcttctgtt ttaaatgcat ttaatccaac aatcacatct gttccgctat
     5941 caatgcgtcc ttgttttttg gcagcagctt cttcaatgcg catttttgga attcctgctt
     6001 caatggcttt ctgcatgccg ccgaggtttt caacttcctg aatcagcttc catgcatcgt
     6061 gaatcaattt atctgtgagt gcttcaacaa tattactccc accccaggga tcaacagtat
     6121 cacaaatacc tgtttccttc tgcaaataaa tttgtgtatt tcttgcaatg cgggcagaaa
     6181 aatcggtggg taatgcaatg gcctcatcca aactattggt atgcaaactt tgtgtacctc
     6241 ccaaggctgc agctaacgct tcaatgcatg ttcgtgctac attattaaat ggttgttgtt
     6301 cggtaaggct ccatcctgat gtttgacaat gtgtacgcag catcagtgat ttattgtttt
     6361 tcggtgcaaa ttgctccatc agcctacacc atagtacacg cgctgctctc agcttcgcaa
     6421 cttccataaa atggtccatg ccgatgccaa agaaaaaaga aagtcgcggt gcgaaatcat
     6481 caacttgcat acctgctgcg attcctgtgc gcacatattc aacaccatct gcaagggtat
     6541 atgccaattc aatttccggc gtggcacctg cttcctgcat gtgataaccg gaaatggaaa
     6601 tggaattaaa tttcggcata ttttttgcgc aataatcaaa aatatctgcc actatgcgca
     6661 tgcttgaaac aggagggtag atgtaagtat tgcgcaccat gaattctttc agaatatcat
     6721 tttgtatagt gcctgccaat tgttgtggtg ttacaccttg ctcttcagcc gcaataatat
     6781 aaaatgccat gataggaatt acagctccgt tcatggtcat gcttacactc atttgatcaa
     6841 gtggtatttg atcaaataaa attttcatat cctcaaccgt atcaatagca acacctgcca
     6901 tgccgacatc tccaaaaaca cgtggattat cagaatcata tccacgatgt gtggcaagat
     6961 caaatgctac actcaatccc ttttgtcctg cagcaagatt tttacgataa aaagcattgg
     7021 atgcttcggc ggttgaaaat cctgcatatt gcctgatggt ccacggctgc attacataca
     7081 tggtgctgta tgggcctcgc aaaaatggtg cagatccggg aagagatttt tttacgacat
     7141 caaaacctgc atccggttga acttgtaccg atggcaattg ataatgatga taaggaatat
     7201 tgcgcaatga tcgtttactc atgttcaggc attgtgtgtt tcaataaaaa ttgaccatgc
     7261 cttttctcca aattggcgag tcaatgattc gatatagtag ctgccgtttg ctgcatcgcg
     7321 atagcgatcc atataacttt cgtaccgtaa taaattctga atgtttatag ccaggcgaac
     7381 actttgtgct tcctgttgta aatcgaaagt aggaatcgat agtgcgtcaa cacctccgag
     7441 caatgcactc atggctgctg tggtgctgcg caataaattg ctgtgtttgt cttcttgggt
     7501 aaatgaatgg gttgaaacaa cggcatgtat gtatggtgtg ggaataaaat ccaattcggg
     7561 atcgtaagct ttcaccagat tattccataa gattttgaat gcacgcaatt tcgccatttc
     7621 gaagaaataa tttgtgccaa caccaaatcg gaaggtgaac agttgtgtta tttcaaataa
     7681 tgaaagattg cgtttctcca tttcttcaaa ataaacagcg gtgcgatgta atgctttcac
     7741 taattgatca atcgatgaag caccggcatc tgcaaggtct gtggtatcta ctttaattaa
     7801 tcggcagtct tgcaggtggc cgttaattct caaaattgca tcagcaagcc tgtcgaattg
     7861 cgcattggta gaagcgggat atccattctt ttcccaatat gaaatcggat cgaattgtat
     7921 cgattgaatc agtctgtcgc gatcaccaag aaaaccgatg cgttcgcgcc agcttccggc
     7981 agtgtgttcg ttgatcggac ctgttgcatc aaaatgtaaa ttcgcaggtg atcccatttt
     8041 tctgatgccg tcaaaaatag catcgtagtc gctatcaact tcagcatgta atcgcagatc
     8101 aataacacca cgctgacggg cttgctgcac aagctcatgc atatttgtcg aagcggagac
     8161 gtcccaatcc tccgtaatat tccaggtgag atgttgttga tgttgattgc cgacttctgc
     8221 ggcaaagtcg tcatccatat ccgcaaggaa atcagcattg gcatcaggct caaattcggg
     8281 aaggtctgat gctgtataaa gaggcataat ctcgatacct tcctttatat gaaccagctt
     8341 ttcccgaaag tcggcacctt tcaggtcttt tgtcacctgt tgcagccatg gagccttttc
     8401 aatatcattg tttataagct cgtccatctt gatgtattgg gttgcaaaga tacggactat
     8461 tgcacttgtg tatcatgtca ttatctgatc gaaagtccta tctttataga tacaaacccc
     8521 cgatgaaaac tgtttatcgt aaactcgaag cctattctgc tgctgcaatt gcactaatgg
     8581 ctgttaaaac ggcagaagca caagtgcatt atgctgatgt agatcctgat atcgtgcttg
     8641 attcacccga tgcattcgct ttgattgacc tcaatggcga tggtcttgct gatattaaat
     8701 tcgctaatca gtctttttat ttgttgctgt ggactttcta tagtgaagaa aagccatacc
     8761 tgtttcaata tatcaatgcc tcggcttatg ctgaactatt tgcctataat gaaaactcgg
     8821 tagcttatac cggtagtgaa ggtggacttt caggtggtgt atggcatgta gcaaaggcta
     8881 tagaggaagg cgaggtggtt aatgctgaat tagattttga ggaatggggc gtgcccatgg
     8941 ctgaaaaaat ttatagttgt acgcttaccg caggatttgg agctacttcc tgcgtgttct
     9001 ccgattctgt tggaaagtgg caacctgaat tggcaaatcg ttttattgga ttgcatttca
     9061 ctaatattac cgatatagga tatttcggtt ggatacgatg ttctatcgcc aacgacggac
     9121 gaaaacttac cattcacgat tacgcctaca atgccactcc cgaacaggca ataattgcag
     9181 gcgatacact tgctgttggt atttctgagg caactttaca gggaactcaa atgtatactg
     9241 acggcaataa tgttttgcat gcaaaaacga cgtcatcggg aaacacccgt ttgcagcttt
     9301 ttgatatcag cggtcgtgag attgtattct tgcaaaataa tattggcgaa tttaattatg
     9361 atctcactgc tttacctgcg ggaccatata ttgttcgttt aacacaaggt gcatcctcct
     9421 caacgattaa aattattcgt aaatgaacgc atggattaaa ccggcaatat taacaatgtg
     9481 tttcatttgc agcaataaat tgcaggcgca gtggtcaacg gttgactcaa catttagtga
     9541 tgacgggatt gcaattattt cagcaggtgc cgggaatgac agatgctata cttccgcggt
     9601 gcaaagtgat ggcaaactca ttattgttgg aaatagcatc tatgaaagcg gcggttattt
     9661 tatttccata tcgagattat ttcccgacgg cacccctgac cctgcatttg gtaccggcgg
     9721 cattgcaatt caatatgtaa acggcgatgg ttatggctca ccgttaatgg gcagtgtaga
     9781 aaaagtattg atacaacctg atgagaaaat tgtgttggtg gggcaggttg tgaatacggc
     9841 tgttgatgag tttaattatg actatttcat catgcgacta atgcctgatg gctcacgaga
     9901 tgctacattc ggcacagacg gattttctat gtatggttat tttgatgaat ggggttattt
     9961 tcaacgagat tacgcacggt ctgccgcttt gctgcctgat ggaaaaattg tgattgttgg
    10021 tgatacggga ccatacgggc tcagctacgc atctattatg cggatacttc cgaatggact
    10081 ggttgatcca acttttggca ccgatggcag ttattttatt aatgtaagcg atgattcata
    10141 tggccgcggt attgctgtac ttggtgatgg cagtatggtc atgtccggat atttttttga
    10201 cggtcaatat gtgttcttcg caaaatggaa tgctgatggc ttgatggatg aaactttcgg
    10261 tatagccggc atccaaatag attctaccat agcgggcttc aaccttaaaa gaatggcaat
    10321 tactcccgat gaaaaattta ttttaatggg agaaaagaat tccacagtaa tgataaacag
    10381 atatacatca gagggaattg cagatactac tttcgcctat tttgaactgc ctcattcggg
    10441 tactatttat tatgccgaag atttgtttat tgatgaagaa aaccacacat atttttgtta
    10501 tcatgcatca aatccaaagg attattttct tggcaagctg acagatgtcg gcggtttgga
    10561 ttattccttc aatgcttcag gtactgccca attcgatctc acaaccggta taagcgaatg
    10621 tcaaagcgta gtgatgtcgg gtgaaacttt gtatgcttca ggatatgcag attttcaaaa
    10681 tgattatgat attgccacaa ttgctgtaac tactgaaggt gaaattgata atggcttcgc
    10741 aacagatggt gtgtggcgcg caaatgtcgg gcgttctgct gattatggac acgccattgc
    10801 attgacaccg gataataaaa ttttaattgc cggaaataac gggtttgtgc ttgtggcaag
    10861 agtaaatgaa acgggtgttt tggattctac ctttggaaat aacggtgttg cttcacatgc
    10921 attgtattac ggaattagct atcccacagt tacaagtgta ctgagcctgc caaatggcag
    10981 aatagtcgtt ctggcctatg acgatgatct ggtagtttca agattatatc ctgatggtag
    11041 tttagatccg actttcaatg gtggctatac caatttgacg gtaggggatg aagaattata
    11101 ttacacttac gacctcgcat atcaggaaga tgaaaaagtg cttttttccg gaatttatta
    11161 tggttacggt ttcgcttatg aagaagctgt ttttttaatt ggaagattaa atgaagacgg
    11221 cactttcgat tcaacgttta atggagatgg tttaaattat gatgcaaatt tttctgagag
    11281 tttaaatatt gctcgtgcaa ttgccgtaat gccggacgat agaattgttg ccggaggata
    11341 ttatgtcaac gacaatgaaa agcaatatcc tcgcttggtg cgctatatgc ctgacggatc
    11401 gcgggataat tcatttagtg gggatggtgc atttggtgct acaatcaatg gtgaggaagg
    11461 gagaatttta gacttgaaag ttgatattga aaatcgcatc gtcggagccg gttatgtagg
    11521 agcagttgag aatgacactg tccaaatcat ggtgatgcgt gtaactgccg acggcgaatt
    11581 ggatactact tttgccgatg gcggctattt tatttatgat acgccactcg aagcttcggc
    11641 gcgatcaata gcagttgacg acagcaacag aatagttgtt agtggatatg cttatatcga
    11701 aattggcaat gccgcatttt tcacgcttcg attattaagt gacggcacac ctgatccgac
    11761 atttggcatc aatggcatgc tgtttactga tttaacaccc gaagacgaca tcgcctatag
    11821 catgcaaata caagatgatg gtaaaattat tttgggcgga acatccagtg aaaatctgaa
    11881 caccaatttt gcattagtac gtctattgcc tgaaaatgga tttactgaac ccattgatag
    11941 cgataccact tatggttcat ttttagtttt tccaaatcct attcacggtg ctttcgtatt
    12001 gcaatatcaa ttgcttgatg cgcagcaggt ttcaatagga ttatttgatg ccgcgggaaa
    12061 taatgttgca caacttatga ccaatgaagc acgtgatgcc ggcgaccatc aggaagcatt
    12121 gcaattgcct gaaacactgg caagaggata ctattttctt cgcatttcgt gcagcgacct
    12181 tgtaacaatg gtgcaaattg tggtgatgta gttaacaccc aatgtgcatc acgcaccatg
    12241 gaaagtagaa ttgcttctta tctttgccac aaacactgct gataatgcaa ctcgaaaatc
    12301 ttgccatcga cataagggaa aacattcttt acatcactat taacaggccc aagaacctga
    12361 atgcactcaa tattaaaact attgttgagt tagatgaggt attcgaggaa tacatgtata
    12421 acgacgatgt aaaaggtgtg attataacag gttccggcga gaagtcattt gttgcaggtg
    12481 ccgatatcag cgagtttgcc gatttcaccc cggagcaggg aagaaccatg agcgaaaatg
    12541 gtcatgatac tttcgataag atcgagatgt ataacaaacc tgtcattgct gcagtgaatg
    12601 gttatgccct cggcggtggc tgtgaattgg ctatggcatg tcatatccga gtggcttctg
    12661 agaatgccat cttcggtcag ccggaagtta accttggtat cattccgggt tatggcggaa
    12721 ctcagcgttt ggttgagctt atcggaaaag gtaaggcact ggaactcctc atgaccggcg
    12781 attctattaa cgctgctttg gcgaaagagc tcggattggt taactacgtg gtttcaccgg
    12841 aagagttgat gcccaaatgt gaggagatca tgaaaaagat cataggcaaa ggtccggttg
    12901 cggtgagcaa ggttgttcaa tgcgtaaacg ctcatttccg cggcggcggc tatgttggcg
    12961 atagctatca gacggagatc acacagtttg gacaatgcat ggaaacggaa gattttgttg
    13021 aaggtactac cgctttcctc aataagcgca aacccgagtt taagggtaaa taagcgttat
    13081 cattttggct ttaacaatgt ctgtgaccta atcacgggca gataaccata tatcttaagt
    13141 aatagatttc gtatcttggc tattcaattt cattagcttg tatttcaaca agttacaatt
    13201 gaagttgtta tatttcgaat gcgcaattat gattggccac aggtatatgc actcgttcag
    13261 gcgatgacgc ggaacgaaaa gggatatttt cgaaaattac atgaggggtt cggaggcgac
    13321 agtcaacgtc agtatatgca gttgtatgca ttgctcgaaa aacaaaagac tcttgatttg
    13381 ggtgcaatca aaaaagcatt tgcaggtcag ggaataaacc tcacgtcgac acgcaaccac
    13441 ctcatgcagc aattgttgcg aagtttgcgc aattattccg tggggaaaag tgacttgatg
    13501 cagcttcgcg aattattaga ttatgcagca ttgctggaaa aaaagggatt gcatcaagct
    13561 tgtatggaat tgacaaagga gggtatcgac ctagcccaaa aaaataacca tccatggtac
    13621 gtgattttat tgctcaacat acagcgttca ataatagatt taatgcctga aaatgaacgc
    13681 gatgctctgg cagctgccat ttatcaaaaa acggttgaag caaataagtc actacaaacc
    13741 tatcggtggg atgtgggaga taagcatcgg cataagggaa aaggaaatca gaactaacag
    13801 gttacggcaa ggggctactg tatggtgccc aaccggtttg ccgattaaca ttggatggag
    13861 taatcagaaa aaaggcgcga tatttgtatg aattaatgct atgaaaaagc gaatcagtct
    13921 gtttttattg accgcaattc ttggattttc ccacttttcg aatgcccaag taaatgccgt
    13981 aaagcctttt aaaaccgtaa taatcggtaa ccaggaatgg atgtcggtga actgggatta
    14041 caaaacacct aaaagctttt tctataataa tgattctacc atagacagta aatacggccg
    14101 actgtatttt tatagcaatg cggtagctgc tgcgccaccg ggatggcatt tgccaacttt
    14161 ggatgaatgg tgggaattaa tgaactacct cggaggcatc aataaggctg caccgctcat
    14221 gatggcttct gataccatag gtctgcattt ggtttccggg ggttacaaaa gcgctaatat
    14281 atcagcagat aatagcgatc tctatggctt tattgaccaa tatgcttttt attggaccgc
    14341 aacacctgat gcggaacaaa cagcttatgc tattcgtatt accccttcaa ccggcgaaat
    14401 tgaggtaaat tccttccgtc gcgcaaatgg tttttctgtg cggtatgtga aagacaaaga
    14461 ggtgaaagag gattgatatc ccaacgcctt ttatccttat tctcccaatg ccgttttcag
    14521 ttttgcaaga tcgaattttg atgcaaatgc taactcatct gcctcagtag caaatcccga
    14581 tagctgcagt gtaatcaatg tttgtcccaa cggaatttga atttcggtag aacgaatctg
    14641 tatttcaggt ttgccgtctt caacaaagcg atcttccatt tctgatttta aaatcgcgcg
    14701 ctgtgtgcca actttcacac ttttatatcc ttcgccaagc atagcaggat tgttcatgta
    14761 catgcccatg gtggctatca tcggtgagtt agccacgata ttgatttcgg cgtcattatt
    14821 tttattggta ggattggaat atgatttact gatcataatg ccaccaccca tcatacccat
    14881 ggccgcggag tttgttgttg catcaccatc agcttttaat ccattgatct cggcaggtaa
    14941 tgattctgca atttgttgtc cgatcaaatt attcaactca ttgatggcat cctgcaaact
    15001 gagctgtgca tttttgtaat ctttggctgc aatatatgtt tgtgcatctt tgatataggt
    15061 gtctatctgg tcttgtccaa ttgcatagtt gcttaatgta aacaggaata gcaccgtcaa
    15121 tatttttttc atatacgtgg attttatgta tcgtaaaggt acagtacaat cctattccct
    15181 gagcaattaa tattatgata ttaatcactc tgcaacatgg gcattagctt attatcagga
    15241 cgattccttc tccttgcgct tcattgctgt cagggtaacc cgtatctcaa aatagctgta
    15301 ggcatcaccc agcgcttctt ttaatatggt gagcttggca tcggggtttt cctgtcgcac
    15361 ttggatgatc cttttcattt tgtcaggact gatgaacttt tcaactgata tcaaaccctc
    15421 tgctaagcac tcggtaagat gtccttctat ggtattgggc gataattcgc gaatagccgc
    15481 aatttcgcct atgctttttc ccgtttgaaa gatttctagg gtctcct
//
LOCUS       DAOQHY010000075        15484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1819_length_15484_cov_3.802061, whole genome shotgun sequence.
ACCESSION   DAOQHY010000075 DAOQHY010000000
VERSION     DAOQHY010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15484
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1819_length_15484_cov_3.802061"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(327..698)
                     /locus_tag="PLL28_07710"
     CDS             complement(327..698)
                     /locus_tag="PLL28_07710"
                     /inference="COORDINATES: protein motif:HMM:NF021237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module-associated protein
                     Cmr5"
                     /protein_id="HNF69248.1"
                     /translation="MNYRNLNHSRTEFAINFITGDCVDPSEMSALIQAMPSLIRTNGL
                     GNAMIFLKQVKGDRRKGNGMQLYINVFSEWLRHLQILGPNADLINTIQIADQNWYIEA
                     KAESYKMANILRFVYSQMFDK"
     gene            complement(679..1590)
                     /gene="cmr4"
                     /locus_tag="PLL28_07715"
     CDS             complement(679..1590)
                     /gene="cmr4"
                     /locus_tag="PLL28_07715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr4"
                     /protein_id="HNF69249.1"
                     /translation="MTTIGKTIILRCEQAFHPGTGNGTGFIDKPVQRSVTTELPIFWD
                     SGLKGSMKEANRPNTAFTPDEHKLLFGSDDNGSQPSAGLFSEAKVLLFPVRTAKGLFT
                     WVTCPYVLNKFIAAQHIRSKYATKIKTSYPDPLNIFTAYCCNTEGVEGMSIAKQVAGT
                     YKAGLEEFIVEVRNDENYKGLSEVLAGEVYPEEGLRYWHDHLKNNLLLISNELFQHLT
                     YFDTEVRTRNKIKNGVAASTGLFVTEFLPEMTVLYAEMSVFQNADTVLTITTKLDNLK
                     STLFAGGSITIGKGLLNLHIIHELQES"
     gene            complement(1587..2738)
                     /locus_tag="PLL28_07720"
     CDS             complement(1587..2738)
                     /locus_tag="PLL28_07720"
                     /inference="COORDINATES: protein motif:HMM:NF021236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module-associated Cmr3 family
                     protein"
                     /protein_id="HNF69250.1"
                     /translation="MHYTLSIVPEDLVFFRGSASFDAGSDNYATSILPPAPSVIYGAL
                     QSISYNDSGINTGNIWPDPSMHISTITLYVQKCNQQIFLFPLPADLIAGRIDNEIKVV
                     NHLKIDEFSNYGVISENGPILIPDKDPLEFDAYRKVNRTELYIDQDGMRQYLVGLYPE
                     QVYNISDLSAKETRTGLGREIGKRIAEEGKLYRIELIRTKTGGIFNAESSLAFHIRMN
                     SSHPSYKPLVNHGEINIRLGGERKTATVRNLSFGAIHTINDRLEKGDFIKAVLTTPFV
                     PKSDPFIEGYWNSIGCTLMTMCSDKELTISGWDMINMRPRQSYKAVSGGAIYFLKIDN
                     INTFKSMTAYHKTGNAQIEAAYMPDTNHANHYLQGFGQYFFAKNILSKA"
     gene            complement(2740..4665)
                     /locus_tag="PLL28_07725"
     CDS             complement(2740..4665)
                     /locus_tag="PLL28_07725"
                     /inference="COORDINATES: protein motif:HMM:NF023885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR-associated protein Cas10/Cmr2"
                     /protein_id="HNF69251.1"
                     /translation="MHIKYLLHISIGPVQSFIEQARKTQDLFAGSALITLLRIEAMKA
                     IALYLKEKSELDKLEFITPFVANNNELVTDDTLGIDFFDKAKKPAQHLNKLECLISLQ
                     DIDVDELRTKTTGAILIFWNTHVDKVCEKWGIDKSTIQDQIATFPTITFLCNRYEDNE
                     VSYAQAFAQQQTVLAGIKRTNSFTQIADGSESGKKCHVDNTFDIRIYRPRRVEYVSVS
                     KGSLDLSDSEKDAIRRKLFAGESLNIITNEERVKIWHLAIGEGLSGISAMKRMVLNEP
                     HSYQSTARIALWRTLKKMQDLRLSEYDAYEELLEKLPHSNDELYFKENLTVQYFNKYE
                     ILGKEVVERDVEPFVSAHDALLTKARSIGLSFTKYYAVFKFDGDNSNKWLSGYWLNKP
                     EFTLQEFHTAYSKAQYDFVRELTEKFKSMCASVVYAGEDFLVFFNIEHFAKVLETIYT
                     AYQTQIVLQLYKFKNPLFTEAQFSFSGGITVAHYKEPLQFVLDKTRAMEKLAKQNGRN
                     MISVAALNASGGENNITIPWITRTNKSLYELIEFVLFHLENKTVSSAFLRELARQDRI
                     YRGAMPMNLYSFFYERTVKRAFNEKNSSGVVLTEPERSKLWDHFREIADYLNWCQQSI
                     LRFFYLLDFIDRKTHEQ"
     gene            complement(4667..5815)
                     /gene="cmr1"
                     /locus_tag="PLL28_07730"
     CDS             complement(4667..5815)
                     /gene="cmr1"
                     /locus_tag="PLL28_07730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr1"
                     /protein_id="HNF69252.1"
                     /translation="MAIIKLEKYMYTLKIPCKVITPMFMEGAIKNSFEFRPASVIGVL
                     RYWMRAILCDKSQQDAFTEIAKYFGKGGENNIAANLRLEVENDDNSSRDFIRKNYFRN
                     NITVVYGENNKPLRTYEGGSYNQRLQYGLSYLFFTKNLGINKNCEYADPGFEFNIVLS
                     SDEIDMLKFGFRALCAASIFGGLGSRTRRGAGCFAIEYSKLLNPIGLPAEMQEVHAWI
                     RTHDKTVDTKPSVYLSRMVKKIWYDDDNCAQMPTFFKEMQMFFHKDRANQPEINSNYK
                     IVLESVGRKFADYRLSIKRNLPKAISYGFPYRDYKSKYKKIERHASPLVITVFKFKDS
                     IITLLYSINEQSLPREFKFNKQHVNDFYSDNNEIINMLNNNFGKYFEK"
     gene            complement(5977..7293)
                     /locus_tag="PLL28_07735"
     CDS             complement(5977..7293)
                     /locus_tag="PLL28_07735"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNF69253.1"
                     /translation="MKTTRFLFQTEFPDSGELLLYNTLNGALIAFEKDRYNQITEILY
                     GGEFNALNNEVANVLYEEGFLVDDNLNEIEIVKKRNALGIADSNRLDVIIMPNMNCNF
                     SCVYCYETHHKSGMDDVTEERLIAWLQKMIPNYKAVLISWFGGEPLLSYPVILRVQEK
                     VQALCKAHNVILHGHITTNGSLLDITKAKNLLALGVHSYQITLDGPAETHNQMRPLKG
                     GGASFEAVHENICALAALEPTPHIKLRVNFDERNVARIRELLHTIPVGLRPSMTLVCE
                     KIFTESFGVVQETMERTAEIEDIQAYAVEIGFRPDHEAMEPNRFTFCYADRANQFTIN
                     YNGDIFKCTVQKFDTKHRLARLGDNGNIEWEAGRLERWMALPAFEEKCEACVFMPLCM
                     GGCRKLRAIFGSVGDDCLAPFKGFDRKVQHYYASTIQESVNQIQIS"
     gene            complement(7460..8524)
                     /locus_tag="PLL28_07740"
     CDS             complement(7460..8524)
                     /locus_tag="PLL28_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69254.1"
                     /translation="MKTFLSFWLTIIMCLGAAQLLGQTAVQYTNGDVFYTRDLQLKPD
                     KIYFKPIGEEPGEYVIVPLTEIANIKFTDGSLMEFNEVVIPATIISNKAELEHSNDSL
                     FRIVEMSLKEVSVQLQSTVQTQNDSLMKVLMMQSRAQKDYYDATLLLMQAQINQLIAQ
                     SFILDSILNTSSSAAELPEIQNGEINWQMPKSSLGIYMFRKPNYDMSNNKPTSIETQV
                     VPNLGCVLSANFNNAARRFRFQIEVGYQVDMSNNGSSSKQAISEVLLGMAFLGQKQYG
                     QTNIYSGLRLEYAGSFHGDETQYMGSGFVTGVEYAPARHLTIGAEATQFVLFNLENSP
                     GNQNTLYGVQPRLHVNWYFE"
     gene            complement(8543..8974)
                     /locus_tag="PLL28_07745"
     CDS             complement(8543..8974)
                     /locus_tag="PLL28_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69255.1"
                     /translation="MRQLLKAWTITTLYMLMSNNIIAQTDLDDKTATVNIIRQTGYSG
                     SATGLKVFIDSAMLCKINTGKYLSIEVLPGVHTLSAQFYGSKPRKSGVPFQFDAEAGS
                     TYYFMITQSVHVASTEVFISRLDENIGKQKMLEKSEDPDCR"
     gene            complement(8985..9581)
                     /locus_tag="PLL28_07750"
     CDS             complement(8985..9581)
                     /locus_tag="PLL28_07750"
                     /inference="COORDINATES: protein motif:HMM:NF025198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HNF69256.1"
                     /translation="MKTTLRTLALATLFITGMSSCARFYGFKQPLAERDPFVPKKSNQ
                     TEQQKIQSEPEIVFNSDEKSIQEATVTPEFLAGADLPRAEVLQLSTTKLSPDITTQTI
                     RNEIQPSIAEKESILAAGEGDDRKMDGFAVAGFVVSLVGLLIFGYVFGAIAIIFSAIS
                     LARISKRKEELKGKGLAVAGLVIGIADIVLLAILMAAL"
     gene            complement(9595..9939)
                     /locus_tag="PLL28_07755"
     CDS             complement(9595..9939)
                     /locus_tag="PLL28_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69257.1"
                     /translation="MATVALKLKGKENVELIAFAREILLGLAGNALISGTKPQLDELA
                     EAINAFESSCLIQGRPEPYQKKTTRLRRAVLELQLTRIASHVERIADGDPKVILDSGL
                     PIRTRQNQIKKS"
     gene            10120..11739
                     /locus_tag="PLL28_07760"
     CDS             10120..11739
                     /locus_tag="PLL28_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69258.1"
                     /translation="MHLFLHNTFEHILEILPTLYRQIHAFSVEEKKTFRKHLTTGRGR
                     KGNSDKVGLFELFADKKMTPEVITLAVFRARCGNQKTKDDFRSSQKELLEELLDFIIR
                     KDPYAQNQLFEREIARADKLVQLREFDLAYFQLEYINKQLAFHDHPYISGLVLRKMQM
                     LIQFVKSKSWKEDFTSLINAEMMLANDAKTRTKAYHLQLIVGMRMSRSWFIKTNEDQT
                     EFLHEISEYEVGSVDSDISFNTFIYISKAHSRVFKMLNDYESAFQLQIKLVSRLEEQS
                     SLLLKQKPVLLFTEYLDAADLCYRTGRTNDVETYLIKAEHASNSCKDDSAMRQILLTH
                     TRCLVYYKYGTNDQLSAAATRLHKALEFRISEASEIQLPVLLSTTMKCHLKLGDKRVI
                     DWCYGVANGLSGEKRRDFIQIIQVIRLCSLYAICKPRDNMVYVDTYPEFQNDVLFVKT
                     QLRGAANEIPLERAVLQCFLQMCQSNRIDQHLKLLSTLMKSLTNMAAKGFIYQEQFYQ
                     VFDVRNWIVEQTERLKCPPNTQKNGHPADNQ"
     gene            11912..12526
                     /locus_tag="PLL28_07765"
     CDS             11912..12526
                     /locus_tag="PLL28_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69259.1"
                     /translation="MNGKIIFRRRSHLTEDEVIAGFLENDSKVVEVVKKEVVKIANYQ
                     VRKMLPKANRDLVNEFAQDALSKLSEVIITKTPSYHKNYLGFCTLCIRNTIFNIKRDP
                     KTIDTEFLEQIAREPRVDDDGDIKHFEMLYSTRLAAMYYCVAALEEPCNNLINLTFLN
                     TDSEKVSARNLSEILDISENNARAKKKRCLDLVRRCMKQKGFYN"
     gene            12537..13319
                     /locus_tag="PLL28_07770"
     CDS             12537..13319
                     /locus_tag="PLL28_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69260.1"
                     /translation="MKNDKEDIQELIDAYLMGDMSTEEATLFTSRINSDPELANQVEA
                     TRHFISLTQQITSGRVTKEHLELQARNEETIRRLFSEQHQTGKSVVMQRALITAAAVI
                     LICIAGYLLMVKPFRNQRIYSHYFSPFEKSMSLSSTDSLFVSHFLELYQNKNYKGFIE
                     SFDPSAMLDTTTTAEGRELITDLSLYLGISYMETGDHERAAEVLTSIDTGSQYYYDAQ
                     WYYCLCLVKSNQTDSILPILNMLVIDSTGYDSVARSLGNEIK"
     gene            13523..>15484
                     /locus_tag="PLL28_07775"
     CDS             13523..>15484
                     /locus_tag="PLL28_07775"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNF69261.1"
                     /translation="MNSENRTNWPTSGEHQDVLSDKSVLVNDDIRTFLNYDRKFIVVA
                     SKGMGKTLLLRYKRKLLELGNKQGQTIVPSDKQLDYVALPPNLSQDFIKVFEDYRFWE
                     DLWEISIAISAIANVILANRESLNSTTLIDEIASIIEDIGLMKPERLKTIAAIKETNF
                     FQPHPSDIITFILNSSYRNYDKFIKKHFARIKMLYTQHIQQAVYIFIDSFDQVLTRTS
                     PHNYIVWNEGQKGLMLAAWNINRQNNHIKVYASVRQEAYAALLDQNRASMFDSVLLLR
                     YSYQDLEQIFIKAIRQYEGKSGIRDFLGIDTIENERALTTESIFHYIYRHSNYTPRSI
                     MAIGNALSSQKINSANSNKARNIKTIVNEVSTELVVKDYLNSEMKPFLSELNNENKID
                     AFLKLINTNILSYDNLKYIKRRYVQNIYGEKYALESDHHPFCDLYNIGLLGKPIFDGV
                     SNKIQQIFKQPYQFDWKMNHILNEGTYYLLHPALNHYISKLNPNFKSIEKLLVGNLCD
                     WTTENENTLSAREYKIFFSHSYYDLDVVNKISESFASQLNLRGIYFSSFIDTIDSKPG
                     VNILHELNKRIDDCNMLIHIVSSNSLKSKNVKKEWLRKYNNEIKTQKLQIISLIIDDT
                     PRSKLPKKLRGKNIKIIDQNDGDFLFNLNK"
BASE COUNT         4767 a         3140 c         2706 g         4871 t
ORIGIN      
        1 tgtgcttttt actattacta atgtgtttgg cttggtgtca caacgagagt gaaaattaga
       61 tgctagaaat ccatcccttg atccttgata caaaagcgtc aaatttcggc cattcagttc
      121 tttctttttc aataactctt taacttttcg aatcaaacac ccgttaataa ttctggagaa
      181 cagaaaatac cttatatcaa tttataatat taattttttt tccaaaaacg gctttgtccg
      241 gcttgacaac ctcaaacatt tcgtcatcta tcaaacgaaa ttctgaatta aggcctgctt
      301 tattaatgca atacactatc atgtgactat ttatcaaaca tctgggagta aacaaatcgt
      361 aatatattgg ccattttata ggactccgct tttgcttcga tataccagtt ttgatctgcg
      421 atttgaatag tgttgattaa atccgcattg gggcctaaaa tttgtagatg ccttaaccat
      481 tccgaaaaca cattgatata caattgcatt ccattacctt ttctccgatc acctttcact
      541 tgctttagaa atatcatcgc gttaccaagt ccatttgtcc ttatcaacga cggcatcgcc
      601 tgtatcaaag cagacatttc gcttggatca acacaatccc cggtaataaa gttgatcgca
      661 aattcagtcc tgctatgatt aagattcctg taattcatga ataatatgta ggtttaataa
      721 tcctttacca attgtgatgc tgccgccggc aaatagagtt gattttaggt tatccaattt
      781 tgtagtaata gttagtacgg tgtctgcatt ctgaaaaaca ctcatttcgg catacaaaac
      841 agtcatctcg ggtaaaaatt cagtgacaaa caatccggtg cttgcggcaa caccattctt
      901 gattttattc cgagtacgca cttccgtatc aaaataagtt aaatgctgaa acaattcatt
      961 tgaaatgagt aataagttgt ttttaagatg gtcgtgccaa tatcttaagc cctcctccgg
     1021 gtacacttca ccagcaagaa cctctgataa acctttataa ttctcgtcat tgcggacttc
     1081 aacaataaat tcttcaaggc ccgctttata ggttcccgca acttgctttg caattgacat
     1141 cccttctact ccttccgtgt tgcagcaata tgcagtaaat atatttagcg ggtcgggata
     1201 acttgtctta attttagttg catacttact tcggatatgt tgagctgcta taaatttatt
     1261 taaaacatag ggacaggtta cccaggtaaa caaccctttt gctgttctaa caggaaacaa
     1321 aagcactttt gcctcagaga aaagtccggc tgaaggttgg ctgccgttgt catccgaccc
     1381 gaagaggagc ttgtgttcat ctggagtgaa tgcagtattt ggtctgttcg cctctttcat
     1441 gctccctttt aacccactgt cccaaaaaat ggggagttcc gttgtaactg atcgctgtac
     1501 tggtttatca ataaaccctg tgccattccc ggtacctggg tggaaggcct gctcacaacg
     1561 cagaattatt gtttttccta ttgttgtcat gcttttgaaa gtatattttt tgcaaagaaa
     1621 tactgcccaa atccctgtaa atagtggttg gcgtggttcg tatcaggcat atatgcggct
     1681 tcaatttgag cattgcccgt cttgtgatat gcagtcatgc ttttaaaggt gtttatatta
     1741 tcaattttca gaaaatatat ggcaccgccc gataccgcct tgtatgattg gcggggcctc
     1801 atatttatca tatcccatcc acttattgtt aactccttat cagaacacat tgtcataagt
     1861 gtgcaaccaa tagaattcca atatccctca ataaacgggt ctgattttgg aacaaacggc
     1921 gtggtgagta cggctttgat aaaatcaccc ttttctaatc tatcattaat tgtatgtatt
     1981 gcaccaaacg acaaattacg cacagttgca gttttccgct ctcctccaag tcgtatgtta
     2041 atctcgccgt gatttacaag aggcttatat gaggggtgac tagaattcat tctaatgtga
     2101 aacgccaatg atgattctgc attaaatatg ccaccggtct ttgttcgaat taattctatt
     2161 ctataaagtt ttccttcctc agcaatccgc ttgccaattt cacgtcctaa ccctgtgcgc
     2221 gtctcctttg ccgataagtc tgaaatatta tacacttgct caggatacaa cccaactaga
     2281 tattggcgca ttccatcctg atcaatatat aattctgtgc gattaacctt cctatatgca
     2341 tcaaattcaa gcggatcctt gtcaggaata agaataggcc cgttttccga tataacccca
     2401 taatttgaga actcgtcaat cttcaaatga tttacaactt ttatctcatt atcaattcga
     2461 cctgcgatta aatctgcagg tagcggaaat aagaatattt gttgattaca cttttgaacg
     2521 tagagcgtaa tcgtgctaat atgcatactt ggatcgggcc atatattacc cgtatttatg
     2581 cctgaatcat tataagagat tgattgtagc gcaccataaa taaccgatgg cgccggcggt
     2641 aaaattgaag tagcatagtt gtctgaacct gcatcaaacg aagcacttcc tcgaaaaaat
     2701 acaaggtctt ccggaacaat tgaaagtgta taatgcatac tattgttcat gagtttttcg
     2761 gtcgataaaa tctaataaat aaaagaacct taaaatgctt tgttgacacc aattaaggta
     2821 atcagcaatt tctcgaaaat gatcccaaag tttagacctc tcgggctctg tgagtacaac
     2881 cccgctgcta ttcttttcat taaaggcacg ctttactgtt cgttcataaa aaaaactgta
     2941 taaattcatt ggcatggctc ccctatatat acgatcctgc ctagcgagtt cacgcaagaa
     3001 ggcagagcta actgtcttat tctctaaatg aaaaagaaca aattcaataa gttcgtacag
     3061 gctcttgttc gttcgagtta tccatgggat cgtaatgttg ttttctcctc cactggcatt
     3121 taacgccgcc accgaaatca tgttgcgacc attctgtttc gccaacttct ccatagccct
     3181 tgttttatcc agcacaaatt gcagaggctc cttatagtgg gcgacagtaa taccgcctga
     3241 aaaactaaac tgtgcctcag taaataatgg gttcttaaat ttatacaatt gtaaaacaat
     3301 ttgtgtttga tatgcggtgt atatagtctc cagcacctta gcaaaatgct ctatattaaa
     3361 gaacactaaa aaatcttctc cggcatacac tactgatgcg cacatacttt tgaatttctc
     3421 tgtcagctcc cgcacaaaat catattgcgc ctttgaatac gcggtatgaa attcttgcaa
     3481 tgtgaattct ggtttgttta accaataccc acttaaccac ttgttactat tatcaccatc
     3541 aaatttgaat accgcgtaat attttgtaaa tgataatcca atggatcttg ccttagtcaa
     3601 tagcgcatca tgggcactga caaagggttc tacatctcgt tcaacaacct cctttcccaa
     3661 gatttcatat ttgttaaagt actgaactgt taagttctcc ttaaaataca attcatcatt
     3721 gctatgcggt aatttttcaa gtaactcctc atatgcgtca tactcgctta atctcaaatc
     3781 ctgcatcttt tttaatgtgc gccacaaagc aatcctcgcg gtactctgat agctatgagg
     3841 ctcattaagc accattcttt tcattgcact aataccactt aagccttcac cgatcgccaa
     3901 atgccaaatt tttacccgtt cctcatttgt gataatattc aaggactcgc cggcaaatag
     3961 ctttcgccta atggcatcct tttcgctatc cgataaatct aaacttcctt tagaaaccga
     4021 tacatattcg actcgtctag gacgatatat tcgtatgtca aaagtattgt caacgtgaca
     4081 ctttttcccg ctttctgatc cgtctgctat ctgagtaaat gagttagttc gtttgattcc
     4141 cgctaacacc gtttgttgct gcgcaaaggc ctgtgcataa gacacttcat tgtcctcata
     4201 ccgattacaa aggaatgtaa tcgtcggaaa tgttgcaatt tgatcttgaa ttgtgctttt
     4261 atctatcccc cacttctcgc acactttgtc aacatgagtg ttccaaaata tcaaaattgc
     4321 ccctgttgtt ttggttctca gttcatctac atcaatatct tgcaaagaaa ttagacactc
     4381 caacttgttc aaatgctgcg ctggtttctt tgctttatcg aaaaaatcta tccctaaagt
     4441 atcatcggta acaagctcgt tgttattggc tacaaacggc gtaataaatt ctaatttatc
     4501 gagctcgctt ttttctttta aatacaatgc tatagccttc attgcctcaa ttcgcaataa
     4561 agtaattaat gcgcttcctg caaataagtc ctgtgttttt cgtgcctgtt ctatgaaact
     4621 ttgaacagga ccgatagaga tgtggagtaa atattttatg tgcatgctat ttttcaaaat
     4681 attttccaaa attattattc aacatgttaa ttatttcatt attatcggag tagaaatcat
     4741 taacatgttg tttattaaac ttaaactcgc gaggcaaact ctgttcgttt atactataaa
     4801 gcaatgttat aatactgtct ttgaatttaa ataccgttat tactaagggc gaagcatgtc
     4861 tttctatttt tttgtacttt gatttataat ctcgataagg aaatccatat gaaatggcct
     4921 ttggtaaatt ccgtttaata gacaatcggt aatcagcaaa ttttcgccca acactttcca
     4981 atacaatttt gtagttggag ttaatttctg gttgattcgc tctgtcttta tgaaaaaaca
     5041 tctgcatctc tttgaagaac gttggcattt gggcgcagtt gtcatcgtca taccaaattt
     5101 tttttaccat ccttgataag taaacactag gcttagtgtc tacggtctta tcatgggttc
     5161 gaatccatgc atggacttcc tgcatttcag ctggaaggcc aattggatta agaagtttgc
     5221 tatattctat agcaaaacag cccgcccctc gcctggttct gctgcccagt ccaccaaaaa
     5281 tagatgccgc acaaagtgcg cgaaacccaa acttcagcat atcgatttca tctgaggaca
     5341 atacaatatt aaactcaaat ccagggtcag catattcaca gtttttattt atgccgaggt
     5401 tttttgtgaa aaacaaatag gacagtccat actgaagcct ttgattgtat gaccctccct
     5461 cataagttct aagtggttta ttgttttcac cataaactac cgtaatgttg tttctaaagt
     5521 agttttttct aataaaatcc ctgctactgt tgtcgtcgtt ttccacctct aaccgaaggt
     5581 ttgctgctat attattctct ccgcctttgc caaaatactt tgcgatctca gtaaaagcat
     5641 cctgctggct tttatcacat aaaatcgcac gcatccaata cctcaataca ccgattactg
     5701 aggccggcct gaattcaaat gaatttttga tagcgccctc catgaacatg ggtgtgatta
     5761 ccttgcacgg gatttttaat gtatacatat acttttctaa tttaatgatt gccatgaggc
     5821 aaatttcatc tgcctcatgg cttttttttg tacatcactt acacccacac aaatggtcaa
     5881 tacaaaagca tgtaaaatgc ttcaattcat ctgcagtcac ctccggtttt acgggtgcgg
     5941 tgatttgggt ttcggttttt ttagatctca cttttttcat gatatctgga tttggttaac
     6001 tgattcttgt attgtagaag cataataatg ctgcaccttg cggtcaaaac ctttgaacgg
     6061 cgcgaggcaa tcatcaccaa cactgccgaa aatggctcgc agtttcctac agccccccat
     6121 gcaaagcggc ataaaaacac aagcttcaca tttttcttca aacgcaggca aggccatcca
     6181 cctttctaaa cgccctgctt cccattcgat atttccgttg tcaccaagcc tcgccaacct
     6241 gtgctttgtg tcaaacttct ggacagtgca tttaaatatg tcaccattat aattaatggt
     6301 gaattgattc gcgcgatctg cataacaaaa cgtaaagcga ttcggctcca tggcttcgtg
     6361 gtcgggcctg aatccaattt ctaccgcata tgcctgaatg tcttctatct ctgctgtacg
     6421 ttccatcgtt tcctgcacta cgccgaagga ttctgtaaaa atcttttcac acaccaaagt
     6481 catcgaaggc ctaaggccta ccggaatggt atgcagtaat tcccttatcc ttgcaacatt
     6541 tctttcatca aaatttactc gtaatttgat gtgcggtgtc ggttccaaag ccgccaaggc
     6601 acaaatattt tcatgcaccg cttcaaaaga cgctccacca ccttttaaag ggcgcatctg
     6661 attatgcgtt tccgcagggc catccaatgt aatctgataa ctgtgtacac ccaatgcaag
     6721 caaatttttt gcctttgtaa tgtccaatag tgatccattg gtggtgatat gtccgtgcaa
     6781 aatcacgtta tgcgctttac atagtgcctg cactttttcc tgcacgcgca gtatcaccgg
     6841 ataactaagc agcggctcgc ccccaaacca actgatcaat acagccttgt aatttggaat
     6901 cattttttgc aaccacgcaa taagacgttc ttcggtcaca tcatccatgc ccgatttatg
     6961 atgagtttcg tagcaataga cacatgaaaa attacaattc atgttaggca tgatgatcac
     7021 atccaggcga ttgctatcag ctatacctaa cgcatttctt ttctttacaa tttcaatttc
     7081 attgaggttg tcatcaacaa gaaatccctc ttcatataat acatttgcta cttcattatt
     7141 caaagcattg aattcgccac cataaagtat ttcagtaatc tgattataac gatctttttc
     7201 aaatgcaatt aatgcaccat taagcgtgtt gtagagcaag agctcgcccg aatcaggaaa
     7261 ttcagtttga aaaaggaatc ttgtggtttt catgcgataa acaaatatct attatctaaa
     7321 atgataaaca aatgatctat aattcatcgc tgtgtttttg cacatgatgt taacagttgg
     7381 ctgcgccgca ctcgcgtttt ccgggtagaa gcaaacttca acttcgttaa agcattgcca
     7441 acaatcaatt catactgttt cattcaaaat accaattcac atgaaggcgt ggttgaaccc
     7501 cataaagagt gttttggttg ccggggctat tttccaaatt gaaaagaaca aattgcgttg
     7561 cttctgctcc aatcgtaaga tgcctcgccg gcgcatattc gactccagtt acaaaccctg
     7621 atcccatata ctgtgtttca tcaccatgaa aactgcccgc atattccaat cttagcccac
     7681 tatagatatt tgtctgacca tattgttttt gtcccaaaaa tgccatacca agtaaaactt
     7741 cagaaatagc ttgttttgaa ctcgatccat tgtttgacat atcaacctgg tatccaactt
     7801 caatttgaaa cctaaatctc ctagctgcat tgttgaaatt agcagacaac acacaaccca
     7861 aatttggaac tacctgtgtt tcaatggagg ttggcttatt gtttgacata tcatagttgg
     7921 gctttctaaa catatatatg ccaaggctgc tttttggcat ttgccaattt atctctccat
     7981 tttgaatctc aggcaattca gcggcactag atgatgtatt tagaatagaa tctaatataa
     8041 aagattgtgc aataagttga ttaatttgcg cttgcattag tagcaatgtg gcatcataat
     8101 agtctttctg tgctcgagat tgcatcatca agaccttcat taatgaatcg ttttgggttt
     8161 gaacggtgct ctgcaattgc acactcacct ctttcaagct catttcaaca atccggaaca
     8221 aactatcatt tgaatgttct aattcagctt tatttgatat aatagtagcg ggaataacga
     8281 cttcattaaa ctccattaat gatccatccg taaatttaat attagcaatt tccgtaagag
     8341 gtacaattac atactctccc ggctcttctc cgataggctt gaaataaatt ttatccggtt
     8401 ttaattgcag atccctagta taaaaaacat cgccattggt gtattgcaca gctgtttgtc
     8461 ctaataattg tgcggcgccc aagcacataa ttattgtcag ccagaacgat aagaacgttt
     8521 tcattttatt ctattttcta tattatctac aatccggatc ttcgcttttt tcaagcattt
     8581 tttgcttacc aatgttctca tcaagtctac taataaacac ctctgtagag gcgacatgca
     8641 cactttgagt aatcataaag taatacgtgc taccggcctc cgcatcaaat tggaatggca
     8701 caccgctttt ccttggcttt gagccataaa actgggctga aagtgtgtgt acgccaggca
     8761 aaacttcaat acttaagtat ttccctgtgt tgattttgca caacatggca ctgtcaataa
     8821 aaaccttcaa tccagtggca cttcccgaat agcctgtttg gcgtataata tttactgtcg
     8881 cagttttgtc atccaaatcg gtttgagcta tgatgttatt ggacattaac atgtataacg
     8941 ttgttatagt ccaagctttt agtagttgtc tcatttcaaa gcatttaaag cgcagccatt
     9001 aatatggcca acaatacaat atcagcaata ccaattacta atccggctac ggccaaaccc
     9061 tttcctttca attcttcttt acgtttactt atacgcgcta gacttatcgc gctaaaaatg
     9121 attgcaatcg cgccaaaaac ataaccgaaa ataagcagtc caactagact aacaacaaaa
     9181 ccagctacgg caaagccatc cattttacga tcatcacctt cacctgcggc caaaatgctt
     9241 tccttttcag caatgcttgg ctgtatttca tttctaattg tctgggtggt aatatcgggg
     9301 gatagtttgg ttgtcgacaa ttgcaacacc tcggctctag gcaagtctgc tccggcaaga
     9361 aattcaggtg tcacagtggc ctcctgaata gatttttcat cagaattaaa tacaatttca
     9421 ggctctgatt ggattttttg ttgctctgtt tgatttgatt ttttggggac aaaggggtct
     9481 cgttccgcca atggttgttt gaacccataa aatcgcgcgc aggagctcat tcctgtgatg
     9541 aaaagtgtgg cgagagcaag tgttcgtaat gtggttttca tttgagttaa ttttttatga
     9601 ttttttaatt tgattttgac gagttctaat tggaagtcca ctatccaata tcactttggg
     9661 atctccatcg gcaatacgtt caacgtgtga ggcgattctt gttaattgca attctagaac
     9721 tgcacggcgc agtcttgttg tctttttctg gtatggctcg ggtctacctt ggattaggca
     9781 ggaagactca aatgcattta ttgcctctgc caattcatct aattgtggct ttgtgccact
     9841 tattaaagcg ttgcctgcaa gtccaagcaa aatttctcgc gcaaacgcaa tcaactctac
     9901 attttctttt cccttcaatt tgagcgcaac cgttgccatc tttctttttc agcaaagaaa
     9961 aacctgtaag agatgtgtaa aatatatttc ttaccgcgtt tccaacaaca aacaaaggcc
    10021 agactgtttg caatttattg gttttcaatt aattacatta tcaattagca ggcacatcaa
    10081 tgacatgtct aagcaaaata ttattgatat gttaattaaa tgcatttatt tttacataac
    10141 acatttgaac acatcttgga aatcctcccc acactatatc gccaaattca tgccttttct
    10201 gttgaggaaa aaaagacttt tagaaagcat ctgacgacag gccggggaag aaaaggtaat
    10261 tcagataagg tgggtctatt tgaattgttt gccgataaaa aaatgacgcc agaggtaatt
    10321 acgcttgctg tttttcgggc aagatgcgga aatcaaaaaa ctaaagatga tttccggtca
    10381 tcacaaaaag aattgttaga agaactttta gattttatta ttcgcaaaga cccctatgcg
    10441 caaaaccaat tatttgagcg cgagatcgct agagcagata agcttgtgca gctaagagaa
    10501 tttgatttgg cgtattttca attagaatac ataaataaac aacttgcttt tcacgatcat
    10561 ccttacatat ccggattggt tcttcgaaaa atgcaaatgc tcatacaatt cgtaaaatca
    10621 aagtcatgga aggaggattt tacctccctt attaacgccg aaatgatgtt ggctaacgat
    10681 gccaaaacta ggacaaaagc ttatcatttg cagttgatag tcggcatgcg aatgagcaga
    10741 agttggttta ttaaaacaaa tgaagatcaa actgagtttt tgcatgaaat ttccgaatat
    10801 gaagttggaa gtgttgattc cgatattagt tttaatactt ttatttatat ttcaaaggct
    10861 catagtcgtg tatttaaaat gctaaacgac tatgagtctg cgtttcaatt gcaaataaaa
    10921 cttgtcagtc gacttgagga acaatcatca cttcttttaa aacaaaaacc tgttcttctt
    10981 tttactgaat acctagatgc cgctgattta tgttatagaa ccggacggac taatgacgtt
    11041 gaaacatatt tgataaaagc tgaacatgct tcaaatagct gcaaggatga tagcgcaatg
    11101 cggcagattt tattaaccca cacccgctgt ctggtttatt ataagtatgg aacgaatgat
    11161 cagctgagcg ccgctgcgac acggcttcat aaagcattag agtttagaat tagtgaagcc
    11221 tcagaaattc aattaccggt tttattatcc acgacaatga agtgtcattt aaaactaggc
    11281 gataaacgcg tgatagattg gtgttatggg gtcgccaatg gtttgagtgg tgaaaaacgt
    11341 cgcgatttca tccaaattat tcaagtgatt agattgtgtt ctttatatgc aatttgtaaa
    11401 cctcgcgaca atatggttta tgttgatact tatcccgagt tccaaaatga cgtgttgttt
    11461 gtaaaaacac agttgcgcgg tgcggcaaat gagatcccat tagagcgagc tgtacttcag
    11521 tgttttttac agatgtgtca aagcaatagg atcgaccaac acctgaagct tttaagtacc
    11581 cttatgaaat ctttaaccaa catggctgca aaaggtttta tttaccaaga acagttttat
    11641 caggttttcg atgtgcgaaa ttggattgta gaacaaacag aacgattaaa atgcccgccg
    11701 aacacccaaa agaatgggca tccggcggac aatcaataaa ctcatggttt ccctaaaccc
    11761 aaaagaccta aaatgatctc ccagatgtcg gcaccactgg tttgcattgc accttgtcca
    11821 accaggactg aagtgcgatt cacggctgca ttttgcatag ataaacagat ttggttcacc
    11881 tatacagtca aataatagca tttcgttaca tatgaatgga aaaataattt ttagaaggcg
    11941 aagccaccta accgaggatg aggtaatagc cggttttctt gaaaacgatt cgaaagtagt
    12001 agaggtcgta aaaaaggaag ttgttaaaat tgccaattat caggtacgaa agatgttacc
    12061 taaagctaat agagatcttg ttaatgaatt tgcacaagac gcattatcta agctttcaga
    12121 ggtgataatt acgaaaactc cgtcatacca taagaattat ttgggatttt gcactttatg
    12181 cattcgaaat accattttca atataaaacg ggatccaaag actattgata cagaatttct
    12241 tgagcaaatt gcaagggaac ctagagtgga tgatgatggt gatattaaac acttcgaaat
    12301 gctatatagc actagacttg ccgcgatgta ttattgcgta gcagctttag aagagccttg
    12361 taataatctt attaatctca cctttctaaa cacagacagc gaaaaggtct cggcaaggaa
    12421 tttatctgaa attttggata tttctgaaaa taatgcccgg gctaagaaaa aaaggtgcct
    12481 agatttagtt cgtcgctgta tgaaacaaaa gggattctac aattaaattg aaagttatga
    12541 aaaacgataa ggaagatata caagaattaa tagatgccta tcttatgggc gacatgtcga
    12601 ctgaagaggc aacattgttt acctcgcgca taaattctga tccggaatta gccaatcagg
    12661 tagaggctac tcgccatttc attagtctaa cacagcagat tacatcaggt agggtaacta
    12721 aggagcattt agaattacaa gctcgtaatg aggaaactat aaggcggtta ttctcagaac
    12781 aacaccaaac gggaaagtca gttgttatgc aacgtgcttt gattaccgca gctgcagtaa
    12841 tattaatctg cattgcgggt tatttattga tggttaaacc ctttcgcaac cagcgaattt
    12901 atagccacta cttcagccca tttgaaaaat caatgtcact atctagtacc gactccctgt
    12961 tcgtgtcaca ctttcttgaa ttgtatcaaa acaaaaacta taagggcttt attgaatcct
    13021 ttgacccttc agcgatgctt gacaccacta ctacggccga gggtcgagag ttgattacgg
    13081 atctttcgtt atatctaggt atttcatata tggaaaccgg agatcacgaa cgagcagcag
    13141 aagtgcttac ctcaatcgat acaggaagcc aatattatta tgatgcacaa tggtattatt
    13201 gtttgtgtct ggtaaaatca aatcaaacgg attcaatatt accaatacta aatatgctag
    13261 ttattgatag cacaggttat gattcggttg cgaggagttt ggggaatgaa ataaagtaac
    13321 ctaataataa cacatccaga acatcgaaca tttatgaaat atgcatatgt aatatatgat
    13381 tgaatcagtc aatatcaaac caatttcaaa ctagcattcc ctaatactaa taaccaactc
    13441 cactgcgact aaattttatt ctaaatgcac ctgctccaat taaacttata atgcgaccct
    13501 ttaacaatta aatgtgtaac tcatgaattc tgaaaataga acaaattggc ccacatctgg
    13561 agaacaccaa gatgtcttat ctgacaaatc cgtgttagtt aacgatgaca taagaacctt
    13621 tctaaactat gatagaaaat ttatagttgt tgcttcaaag ggaatgggca agacattact
    13681 gctaaggtac aaacgcaagc tgctagagct aggtaataaa caaggtcaaa ccattgtccc
    13741 ttcagacaaa caacttgatt atgttgcact tccaccaaat ttatcgcaag actttataaa
    13801 agtatttgaa gactataggt tctgggaaga tttatgggaa atatctattg caatttctgc
    13861 aattgcaaat gtcatattgg caaatcgaga gagcttaaac tcaaccacat taattgatga
    13921 aattgcaagc ataatagagg atattgggct tatgaaaccc gaaagactaa agacaattgc
    13981 tgcaattaaa gaaaccaatt tctttcaacc ccacccctcc gatatcatta ccttcattct
    14041 aaatagcagc tacagaaatt atgataaatt tattaaaaag cattttgccc ggattaaaat
    14101 gctttataca caacatatac aacaagctgt atatatattc attgattctt ttgatcaagt
    14161 attgaccaga actagcccgc acaattatat agtatggaac gaaggacaga aaggattaat
    14221 gttagccgcc tggaacatta ataggcaaaa taatcatata aaggtgtacg catcagttcg
    14281 tcaagaagca tatgctgcgc ttttagatca aaacagggcc tctatgttcg attcggtatt
    14341 gctgctcagg tatagctatc aagatcttga acaaatattt attaaggcaa ttagacaata
    14401 tgaaggaaag tccggaattc gcgatttcct tggtatcgat acaattgaaa atgaacgagc
    14461 tttaaccact gaaagtatct ttcactatat atatcgtcac tcaaattata caccaagatc
    14521 aataatggca atcggaaatg cgttatcctc ccaaaagata aattcagcaa attcaaataa
    14581 agctagaaat ataaaaacca tagttaacga agtgtcaaca gaacttgttg taaaagacta
    14641 tttaaatagt gaaatgaagc cctttctttc ggaattaaat aatgaaaata aaattgacgc
    14701 atttttaaaa ctaataaaca ctaacatatt gtcatatgat aatttaaaat atattaagcg
    14761 cagatatgtt caaaatattt atggggaaaa atatgctctt gagagcgacc accatccatt
    14821 ttgtgattta tataacattg gcttattggg taagcctatt ttcgacggtg tgtcaaataa
    14881 aattcaacaa atatttaagc agccatatca atttgattgg aaaatgaatc atatactcaa
    14941 tgagggcaca tattatctgc tgcatcccgc tttaaatcat tacataagta agctcaatcc
    15001 aaattttaaa tcaattgaaa agcttttggt cggtaactta tgtgattgga caacagaaaa
    15061 cgaaaacaca ttgtctgcaa gagaatataa aatatttttt tctcattcat attatgattt
    15121 ggatgtagtt aacaagatat cagaatcatt cgcgtcgcaa ttgaatttaa ggggtatata
    15181 ttttagttca tttattgata ctatagattc caagccaggg gtcaatatac tgcacgaact
    15241 gaacaaacga attgatgatt gtaacatgct tattcatatt gtaagctcta atagtttgaa
    15301 atctaaaaac gtaaaaaaag aatggcttcg taagtacaat aatgaaatta aaacacagaa
    15361 actacaaatc atcagtctaa taattgacga tactccaaga agcaaactcc ctaaaaagtt
    15421 gcgagggaaa aatataaaaa taatagatca aaacgatggg gattttctat tcaatttaaa
    15481 taaa
//
LOCUS       DAOQHY010000076        15202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1867_length_15202_cov_4.942629, whole genome shotgun sequence.
ACCESSION   DAOQHY010000076 DAOQHY010000000
VERSION     DAOQHY010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15202
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1867_length_15202_cov_4.942629"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..472)
                     /locus_tag="PLL28_07780"
     CDS             complement(89..472)
                     /locus_tag="PLL28_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69262.1"
                     /translation="MSKRKYVKYGIGFLFIAAAMVTLMGYATMYLWNWLIPEIFGGTT
                     VTFWQAIGLIALGKLLTGFMSWGPKSWGKHHMGYKGQHWRAKMEAKLANMTPEEREKF
                     KKYYYDRCGWKTPQDVTENPTNQTA"
     gene            complement(484..1158)
                     /locus_tag="PLL28_07785"
     CDS             complement(484..1158)
                     /locus_tag="PLL28_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNF69263.1"
                     /translation="MPHYLAGSAVYIYNIDDNAFVIWESMDESLHLGRYMTQQQQVII
                     EEAVKRERKKLLQFIRTRVANEEDARDILQDVFYQLASNHTMVETIENMASWLYRVTR
                     NRIIDWYRKRKTDSMDVMAAYDDEGEDGYFTGLATLSTDNSDNPDTLYERQLVWETMY
                     NALAELPEEQREVFILHELENKSFNEIAEITGAPLNTLLSRKRYAVLHLRERLQGLYQ
                     NFVQQD"
     gene            complement(1195..2004)
                     /locus_tag="PLL28_07790"
     CDS             complement(1195..2004)
                     /locus_tag="PLL28_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF69264.1"
                     /translation="MKKTISIIAAAAITGAFVFSACKDGKKGNDEDITIPTLQAAQVA
                     GMQVDTVTFNASDGLEVTGVVYCADTNAPVIVLCHQARFNNYEYAETAPRLVALGFTC
                     LAIDQRSGGDMESHINATNKRAVEKNLPTGYTNSLPDISAAVNYASERFHKQVILWGS
                     SYSAALTLKVAMENPNVRAAVGFSPILKFDSGESAADIFKNYKDKPLFVTSTEKEGAP
                     IAKALSHLGDDVLYQYYPETKGTHGSKALWSSDPSSTYYWEEITKWLNAHK"
     gene            2136..3593
                     /locus_tag="PLL28_07795"
     CDS             2136..3593
                     /locus_tag="PLL28_07795"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNF69265.1"
                     /translation="MKLKSLRITFIFSLALALVVSGCRREIINTDTTAKLRFSEDTVH
                     FDTVFSTVGSVTLSLILFNDFNTTLEVSNITLAGGAFSQFRMNVDGAPGDVFDNIQIP
                     PNDSLYVFVEVTVDPNEEALPYVIEDSILFTTNGNMQTVRLIAYGQNAHFHYGQILGL
                     GPSDDTTWTDDLPHVIIGSVLVDSLFTLHITEGTNIYLHGGSYFYVLGTLDVQGTKDS
                     VVTFQGDRLEDFYKDVPGQWEGIYFLRGSTNNVIKYAEIKNALEGISLGFSTVPDLSS
                     FLDNQVDLTLENTMIYDIQNNGITSLNSVVKATNCLVYNIGASNVALFLGGDYQFRHC
                     TLANYGSTYLSHQSPILGFTDYFEFNIDNILQNDLTKADFYNCIIYGSIAEGDEIVID
                     TLLTPTVFNFNFDHCIMRTTIDESLLNDTGCFYNQDPIFANIEERNYCPTTGSPALNA
                     GIDLPWDPVLIDIRGEVRPFAGTLPDIGAYETTAE"
     gene            3667..4290
                     /locus_tag="PLL28_07800"
     CDS             3667..4290
                     /locus_tag="PLL28_07800"
                     /inference="COORDINATES: protein motif:HMM:NF025691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HNF69266.1"
                     /translation="MLTFNVKHQESYSRGELLLRSFFGWLYILIPHGIAIMFYAIWIY
                     IATILAWWIILFTGKTPEFYYNAMLGFNRWGLRLSARIWNLSDGYPAIGPNGSDDKTE
                     LHYPMIHIGRGQLLLRSIFGFVYIIIPHVICLWFRMIATYFLVFLAWWTVLFTGKYPE
                     NWHKFNVGTLRWQMRLGLYYGWLLDKYPPFSGAPDQMEEAEKPIDQI"
     gene            4408..4836
                     /locus_tag="PLL28_07805"
     CDS             4408..4836
                     /locus_tag="PLL28_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNF69267.1"
                     /translation="MRRYCAYQERCHEEVRTKILSMKVYGDTLERVMDALIQEDFLNE
                     ERFAIAYAGGKFRMKKWGRLKILRELQLRKISTYCINKAMKEITDEAYEKTLKQLIRQ
                     ARKSYSTYNTFEQNHKVSKYLIAKGYEPALVWQYLGKDDH"
     gene            complement(4833..6698)
                     /locus_tag="PLL28_07810"
     CDS             complement(4833..6698)
                     /locus_tag="PLL28_07810"
                     /inference="COORDINATES: protein motif:HMM:NF023459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HNF69268.1"
                     /translation="MKATTLLGLFLLFSKGLFAQILLTGTVTDNQGIPVGFTTITTLQ
                     PVTQYTFANENGLYTLTFPDSMLNKRIQLVFNMIGYFPDTINVELNNKKIVQNKILTE
                     QILLESVVITSDNISGYAYGTTKIGKKGRKSKSPANGYTNTQSWSQVPITTAEEYHKS
                     PENNFKFVNENPVTTMSIDVDNASYSNIRRYLNLGELPPTDAVRVEEMINYFSYQYPK
                     PEEGQPYVVYTEEAPCPWNPEHRLLHVGFKGKTIEAENLPQTNFVFLIDVSGSMGDAN
                     KLQLVKSSMKLLVRNLRDEDRVAIVTYSSNVQVALQSTKCTHKDQILEVIDGLVASGS
                     TAGAAGIQKAYDIAEKNFINSGNNRVILCTDGDFNVGVSSPEELEKLIMINRDKGIFL
                     TCLGFGMGNLKDNKIELLADKGNGNYGYIDNIQEANKLLVETFGATMYTVAKDMKVQV
                     EFNPAYAAAYRLIGYENRVLKNEDFEDDAVDAGELGAGQTVTTLYEIIPAGSKDTLLK
                     NAPKLKYQKTDLTTEALNGEMATVKVRYKEPNGDTSIEAAYPVKISGVAFENTSTDYQ
                     FSAAVALYGMLLEQSQYCGKGDVSQVIKIAEQAKGEDIDGYRAEFVRLVKATPFN"
     gene            complement(6774..7235)
                     /locus_tag="PLL28_07815"
     CDS             complement(6774..7235)
                     /locus_tag="PLL28_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69269.1"
                     /translation="MKNITRNAEQVTVAVVILGLILRLLEIELADTILMIGLLTLSFV
                     YLMFGMLAINNNLQYLPIAQTGDTLTTVAKMISGLCGLAMSIAAIGIMFRILHWPSWQ
                     FMLMTGVVACAVCLLLCYLVVRPQHEKLASFLNYRMVMMLGMSLLLMYSSL"
     gene            complement(7235..7603)
                     /locus_tag="PLL28_07820"
     CDS             complement(7235..7603)
                     /locus_tag="PLL28_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNF69270.1"
                     /translation="MKSLYTIIFLIISNIFMTLAWYGHLQFKKLGWFENSGLFMIILL
                     SWGLAFFEYCFQVPANRIGSSTYGGPYNLFQLKILQEVITLVVFTVMALYVFKTETFR
                     WNYLAGFGCMILAVYFIFKK"
     gene            complement(7768..8319)
                     /locus_tag="PLL28_07825"
     CDS             complement(7768..8319)
                     /locus_tag="PLL28_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69271.1"
                     /translation="MSIVKILRTAILFLILEINAARGVAQSSDNFYVSPAFTIGYTFA
                     GGFAIGYDVDFGIWRDYMSADREFNTGLSYHSLWTQAYYDRWHHIRSINFMMETDYYD
                     AKVGLGYVVDKHGYNHIVHCWAPGFYTDISFTNNEVYFPWIGMQGFWYNWSYFEGFEY
                     PYVTPYVKYKYDFLQQSGIKNFN"
     gene            complement(8561..9523)
                     /locus_tag="PLL28_07830"
     CDS             complement(8561..9523)
                     /locus_tag="PLL28_07830"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HNF69272.1"
                     /translation="MKTLVIALLCISSFALHAQGVTTTTYYNLRYEQVPTKPDEGFIA
                     IITTPDSEKEHWADIKVTTADGILLQQYSYLNLDKGLRRDSIVYNRPDGTKVRRSYFD
                     ASGTLIREYAYDSLEQMTSSYEYEVNYLYSYEDGALESKTHVVNDKLTDSIYYYYPTG
                     KLRLIETFSSFGDYKSKISYHENGEVIYQSTFDENGNETVLIGEKPAEEAPAAKEGIQ
                     EFTEKMPEFPGGVSGLYAYISGHIQYPRAARDNGVEGTVYMKFVVTEDGAVIKAESLR
                     PIFPPMEFEALRVIMNSPKWTPGMMDGKNVAVWYRLPVKFTLED"
     gene            complement(9612..13151)
                     /gene="smc"
                     /locus_tag="PLL28_07835"
     CDS             complement(9612..13151)
                     /gene="smc"
                     /locus_tag="PLL28_07835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNF69273.1"
                     /translation="MKLSTLEIKGFKSFADKTVLHFNENITGVVGPNGCGKSNVVDAI
                     RWVLGEQKPTALRTERMENLIFNGTKNRKSSGMAEVSLTFENTRNLLATEFSTVTVSR
                     HYFKTGESEYRINGVTCRLKDIQSLFMDTGISTDSYSIIELGMLDDILHDKDNSRRKL
                     FEQAAGISKYKSRKKETLNKLGATQLDLDRVEDLLFEIENNLKELEKQAKRAQRYQKL
                     KGEYKLNSIDLAVLTLQSHKDIYKQLSNQQTFESDAKLSIEAEIDALEASIADKKTAL
                     IDKEQSLSKLQKDLNAVVNEIHQKENDTNLSKERTKFLEEKQVSLQKSIEDAEQYIAS
                     TQGDIASLEQRVAEENEKLVGIKQSLQHFLESLEKVKQDHDQVREKLESTQSVFKQAE
                     RELYDIDKKIAINKVRLENYNRELGNIESDRGTQGDKLDPLRQELVAHDENRAAAQQT
                     VDALMKEDQDLKQTIAALQREIEKSRNEVGNARRKLDAKKNEFTLTKNMFESFEGFPE
                     SIKYLKKNVASMKESPLLSDVVSSQDAYKTAIESFLDPWLNYYIVQDMAHAMEAVNEL
                     ATKEKGRANFFLLSEFKSKQQPAAISGVVPALDVVTINEKYAALASHLLGNVYIIAAD
                     EDFAAVSARIASAHPDVHLLHQSGRVARSGKAISGGSVGLFKGKRIGREKTLETLEAE
                     IKTLQEEETLQSNALKEQQTKLSELQQSAVDRQIQQAREELNKVVQQQVSTKARIESI
                     EQLLQQMEDKKVAFMRQIQETDEESIKLNESHDKHMELKEKRQAELLDMEVLYREVAE
                     KLTFHSNQYNQENIKYHQQFNRLQSVQQDLDYKRTRRDEAMITAESSKKQLQDVTEEI
                     AENIEKVAVMQEALLKLYDEKTTMEQMVSREEENYYGSRGEVAETEDKIKALQKKKDS
                     SEHALGSIKDRLNELKVQLAGIKERLWVEFQVELESILDNEPPTDVTLEELQVKVDAA
                     KKRIENYGEVNPMAIQAYEEMNTRYDFIKTQREDLLQAKTSLLDTIKEIDETARNKFD
                     EAFMQIRENFKMVFRSLFNEEDDCDLLLKDDYDPLEAQIEIIAKPKGKKPQVIDQLSG
                     GEKTLTGMSLLFALYLIKPAPFCILDEVDAPLDDNNIMKFNNTIRKFADKSQFIIVTH
                     NKRTMSEVDIIYGVTMPEMGISKVVPVDFRGLN"
     gene            complement(13331..14578)
                     /locus_tag="PLL28_07840"
     CDS             complement(13331..14578)
                     /locus_tag="PLL28_07840"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNF69274.1"
                     /translation="MKVFKFGGASVKDAAGLRNVGTIISSFQDKDIIIVVSAIGKTTN
                     ALEKIAVSAYEGGDYQEALQSLIAQHTGIAAELGLQNGFDFFGDMLVTLAGQNSHRSY
                     PQFYDQIVSIGELMSSSLLSQYLLSAGIANTWVDAREIIKTDNSWREGQVNWEETLKA
                     VQNTLPAALKSGHVVTQGFIGGAPDGFTTTLGREGSDYTAAILAHCTNAEGQWIWKDV
                     PGVLSADPKKYPDAVLLPELTYYEAIEMTYYGASVIHPKTIYPLQQKQIPLYVRSFLH
                     PEESGTVIATDNKFIPYPAVIMHKSGQTLISITPSLTTFVGDMQMANIYAIFSRHKLR
                     INTIQIAAQSVSIVVDYDAYLLESLMADLKASYSKVGVRENTDLQLLTVRHYNEDLLK
                     KLMGDKKVYLEQRSRSTVQFLYE"
BASE COUNT         4089 a         3238 c         3175 g         4700 t
ORIGIN      
        1 aaaaatactt gatacttgat acttgatact tgatacttga taccactcac cactcaccac
       61 ccgcctatcg gcgaggcaag ctcaccactc acgccgtctg attggtgggg ttttcggtta
      121 cgtcttgtgg tgttttccag ccgcagcggt cgtaatagta tttcttgaat ttttcgcgtt
      181 cttcgggcgt catgttagct agcttggctt ccattttggc tctccaatgc tgacctttat
      241 agcccatatg atgtttcccc cagcttttag gaccccagga cataaagccg gtgagcagtt
      301 ttccgagggc aatcagaccg atagcctgcc agaaagtgac ggttgtgccg ccaaaaattt
      361 cgggaattaa ccagttccat aaatacatgg tggcatatcc catgagcgtt accatggcag
      421 cggcgatgaa taaaaagccg ataccgtatt ttacatattt tcgttttgac ataatgttta
      481 gagttaatct tgttgcacaa aattttgata taaaccctgc agacgttcgc gcaggtgcag
      541 cacagcgtag cgtttacgag ataacaaggt attcaaaggt gcaccggtaa tctcggcgat
      601 ctcgttgaag ctcttgttct ccaattcgtg caaaatgaat acctctcgtt gctcctcagg
      661 tagctcggcc aatgcattat acatcgtttc ccaaactaac tgacgttcat acaaagtatc
      721 cggattgtca ctgttgtcgg tagataaggt ggccaaaccg gtaaaataac cgtcttcacc
      781 ctcatcatca taagcagcca tcacatccat ggaatcggtc ttccttttgc ggtaccagtc
      841 gatgatcctg ttgcgggtca cgcggtataa ccatgaagcc atgttttcta tggtctccac
      901 catggtgtga tttgaggcaa gttgataaaa cacgtcctga aggatgtcgc gtgcatcctc
      961 ctcattcgca acccgggtgc gaatgaactg gagcaacttt ttacgttcgc gctttactgc
     1021 ctcctcaata ataacctgtt gttgctgcgt catgtatcgt cccaaatgta aactctcgtc
     1081 catactttcc caaatgacga atgcattatc gtcaatattg taaatgtaaa cagcgctacc
     1141 tgccaaatag tgcggcaaat agcgctgtta aatatctaaa taattgacta tcagctattt
     1201 atgtgcatta agccattttg tgatctcctc ccaataataa gtacttgaag ggtcgctcga
     1261 ccaaagggcc ttagagccat gagtgccttt ggtttcgggg taatattgat ataaaacatc
     1321 atcacctaag tgcgacagtg ctttggcaat tggtgcacct tctttttcgg tagatgtgac
     1381 aaacagtggt ttgtctttat aatttttgaa gatatctgca gctgattcac ctgaatcaaa
     1441 cttcaaaata gggctgaaac caacggcagc gcgcacattt ggattttcca ttgccacttt
     1501 aagggtcaat gccgccgaat agctgcttcc ccaaaggata acttgtttat ggaaacgctc
     1561 tgaagcgtaa ttaaccgcag cggaaatgtc aggcagtgag ttggtatatc ctgtaggaag
     1621 atttttttcc actgcacgct tgtttgtggc attgatatga gattccatat cgccacctga
     1681 acgttggtcg attgccaggc atgtgaaacc aagcgcaacg agtcttggag cagtttctgc
     1741 gtattcgtag ttattaaagc gcgcctggtg acaaagaaca atcacaggag cattggtatc
     1801 ggcacaatat acaactccgg ttacttccaa tccatcggat gcattgaagg tcacggtatc
     1861 tacttgcata ccggcaactt gagcagcctg caaggtgggg atagtaatat cctcatcatt
     1921 gccttttttg ccgtctttgc acgctgaaaa cacaaaagca ccggtgattg cggcagcagc
     1981 gataattgaa attgtttttt tcatagtttc ttttgagtgg aggcaaaatt aagtgaatgc
     2041 aacttgcctc cgaagtaagt gcatgaggcg cgtgtaaaag atttcctaat tttgaggcac
     2101 gaatacgcag aataaatgcg ttaaggtcac cggttatgaa gttgaagtca ctgcgtatta
     2161 ctttcatttt ttcactggca ctcgccttgg ttgtttccgg ttgtcgccgt gagatcatta
     2221 ataccgatac aactgccaaa cttcgcttta gtgaggatac cgtgcatttt gatacggttt
     2281 tttctacagt aggcagcgta acgctcagtt tgattttatt caatgatttt aataccacac
     2341 ttgaagtaag caatattacg cttgcaggtg gtgcattttc gcagttcaga atgaatgtgg
     2401 atggagcacc cggtgatgtt ttcgacaata ttcagattcc gccaaatgat agtctgtacg
     2461 tttttgtaga ggtaactgtc gatccgaatg aagaagcttt gccttatgtt atcgaggata
     2521 gcattctatt tactacaaac ggtaatatgc aaacagtgcg tttgattgct tatggtcaaa
     2581 atgcgcattt tcattatggt cagatactcg gtttaggacc ttcagatgat actacctgga
     2641 ctgatgattt gccacatgta attattggat ctgttttggt ggatagttta tttaccttgc
     2701 atatcaccga aggcacaaat atttatctcc atggaggcag ttatttttac gtgttaggca
     2761 cattggatgt tcaaggaacg aaagatagcg tggtaacatt tcagggcgac aggcttgaag
     2821 atttttataa agatgtcccc ggtcaatggg agggaattta tttcctgaga ggcagcacaa
     2881 ataatgtcat taagtatgct gaaataaaaa acgctctgga agggatatca cttggatttt
     2941 ctacagttcc ggatttatca tcattcctcg ataatcaggt agaccttact ttggaaaaca
     3001 ccatgattta tgatattcag aataatggta tcacttcttt aaatagtgtg gtgaaggcaa
     3061 ctaattgtct tgtttataat attggtgctt caaatgtagc attgttcctt ggtggcgatt
     3121 atcaatttcg tcattgtacg cttgcgaatt atggttctac ctatttgtca catcagtcgc
     3181 cgatacttgg tttcaccgat tatttcgaat tcaacatcga caatatttta caaaacgatc
     3241 taacgaaagc agatttttac aattgtatca tatatggttc gatagcagaa ggcgacgaaa
     3301 ttgtgataga tacattattg acaccaacgg ttttcaattt caactttgac cattgtatca
     3361 tgcgcaccac catcgatgag tcgttgttga atgatacagg ttgtttttac aatcaagatc
     3421 ccatctttgc caatattgaa gagcgcaact attgtcccac aaccggttcg cctgcgttga
     3481 atgccggtat tgatcttccc tgggatccgg tgctaatcga catcagaggc gaagtcaggc
     3541 catttgccgg aacgcttccc gacataggcg cctacgaaac cacggcagag taggggaata
     3601 actccattag gaattgtcgt tccgtttggg tagatttgat tattgttcat actcaaatac
     3661 cctgttatgc taacctttaa tgtaaaacac caggagtcat attcacgagg tgaattattg
     3721 ctccgatcat tttttggatg gttgtacatc cttattccgc atggcattgc catcatgttc
     3781 tatgccatat ggatttatat tgccactatt ctagcttggt ggattattct tttcacaggt
     3841 aaaacaccgg aattttatta caatgccatg ctcggcttta atcgttgggg attaagatta
     3901 tcggcacgca tctggaatct ttcagatggt tatccggcaa taggtccgaa tggtagcgat
     3961 gacaagaccg aattacatta cccgatgata catatcggtc gcggacagtt attgctgcgt
     4021 tctatattcg gttttgttta tattattatt ccacatgtga tctgtttgtg gtttcgcatg
     4081 atcgccactt atttccttgt gtttttggca tggtggaccg tgcttttcac cggaaaatat
     4141 cctgagaact ggcataagtt taatgtaggt accctgcgtt ggcagatgcg tcttggacta
     4201 tattatggtt ggttgctcga taaatatcct ccattttcag gagctcccga tcaaatggaa
     4261 gaggccgaaa aacctattga tcaaatttga ttttagcatc taaaaaagat aaaccggagt
     4321 agttatgctc cggttttttt tgtgtcttta tatttgtagg ataattgttt tcgaattagt
     4381 gtcatgtacg aagaagtgct aaataaaatg cgaaggtatt gcgcctatca ggagcgatgc
     4441 catgaggagg tgcggacaaa aatcttgtcg atgaaagtgt atggcgatac attggaacgc
     4501 gtgatggacg ctttgataca ggaagatttt ttgaatgaag agcgctttgc cattgcttat
     4561 gcgggaggaa aattccgcat gaaaaaatgg ggccgactca aaatacttcg agagttgcag
     4621 ttgcgtaaaa tctccactta ttgtatcaat aaagctatga aggaaattac ggatgaagcg
     4681 tatgagaaaa cattaaagca gcttatacgc caggctagaa aatcttattc aacatacaat
     4741 accttcgaac agaaccataa agtttcaaaa taccttattg caaagggata tgaacctgcg
     4801 ctggtatggc aatatcttgg caaggatgat cattaattaa atggcgtagc cttaaccaaa
     4861 cgaacgaact ccgcccggta accatcaata tcttcacctt tcgcttgttc agctattttt
     4921 attacttgac tcacatctcc ttttccgcaa tattgtgatt gttccaatag catgccatac
     4981 aatgccactg ctgccgaaaa ttggtaatca gtacttgtat tctcaaaggc aacacctgat
     5041 atttttacag gatatgcagc ttcaatactt gtatcgccat tcggttcttt atatcgcact
     5101 tttacagtgg ccatttcacc gttgagggct tcggttgtta ggtctgtttt ttgatatttt
     5161 agtttaggtg cgtttttcaa taaagtgtct ttagaacctg ccggaattat ttcatataaa
     5221 gtggtcaccg tttgccctgc tcctagttcc cctgcatcta ctgcatcatc ttcaaaatcc
     5281 tcatttttaa gcaccctgtt ttcataacca attaaccgat aagcggcggc atacgcagga
     5341 ttgaattcca cttgcacttt catgtctttc gccacggtat acatggtagc gccaaaagtt
     5401 tcaactaaga gcttgtttgc ttcttgtatg ttgtcgatat aaccataatt accatttcct
     5461 ttatcggcta gcaactcaat tttgttgtcc tttaagttgc ccatgccaaa accaagacag
     5521 gtaagaaaaa ttcctttatc gcgattaatc attatcaatt tctctaattc ctcagggctt
     5581 gaaacgccta cattaaaatc gccatcagta cacaatataa cgcgattgtt tccactatta
     5641 ataaaattct tttctgcgat atcataggct ttttgaatgc ctgcggcacc tgctgttgat
     5701 ccagaagcta caagaccgtc aatcacctcg agtatttgat ctttatgtgt gcattttgta
     5761 ctctgcaatg caacttgaac attagatgag taggtaacga tggccactcg atcttcatcg
     5821 cgcaaatttc ttacgagtaa tttcattgac gattttacca attgtaattt attggcatca
     5881 cccatacttc cggaaacatc aatcagaaat acaaaatttg tttgaggtaa attttccgct
     5941 tctatggttt tgcctttaaa tccaacatgc agcaagcgat gttcgggatt ccacggacaa
     6001 ggggcttctt cagtatatac cacataaggc tgaccttctt caggctttgg atattggtaa
     6061 gagaaataat ttatcatttc ctccacgcgc actgcgtctg taggtggtaa ttctcctaaa
     6121 ttcaaatacc gacgaatatt actataagat gcattgtcga catcaataga catcgtagtt
     6181 acaggatttt catttacaaa tttgaaattg ttttctggac tcttatgata ttcttcggct
     6241 gttgtaatgg ggacttgaga ccaggattgg gtattggtat atccatttgc cggagattta
     6301 ctttttctcc cctttttgcc aattttggta gttccatatg cataaccaga tatattatca
     6361 gaagtgatta caacactttc caaaagaatt tgctcagtca gaatcttgtt ttgaacaatt
     6421 tttttgttgt tcaattcaac attgatggtg tccggaaaat aaccgatcat attgaatacc
     6481 agttgtatac gcttgttaag cattgaatcc ggaaaggtca gggtgtataa gccattttca
     6541 ttggcaaaag tgtattgagt gaccggttgt aaagtcgtga tagtagtgaa accaacgggg
     6601 attccctggt tgtcggttac ggtacctgta agtagaatct gtgcaaacag gcctttgctg
     6661 aaaagcagga aaagaccaag tagggtagtg gctttcatga tgtaggttta tgtattagga
     6721 acgaaacttt tgataacatg tttcattcct tttgttatga aattgtcatt tcgctataaa
     6781 ctactgtaca tcagcagcaa actcattccc agcatcatca ccattctgta gttaagaaaa
     6841 gaagctagtt tttcatgttg cggtcgaact actaaataac ataatagtaa acaaacggca
     6901 catgcaacta cccccgtcat caacataaat tgccagcttg gccaatgtag tatgcgaaac
     6961 ataataccaa ttgctgcaat cgacattgca agtccgcaaa gaccggatat cattttcgca
     7021 actgttgtaa gtgtgtcacc ggtttgggca attggcagat actgcaagtt attattgatg
     7081 gcaagcatgc cgaacattaa ataaacgaaa ctcaaagtta ataggccgat catcaaaatc
     7141 gtatcagcaa gttcaatttc cagtagtctt aaaatcagtc ccaaaatgac cactgcaaca
     7201 gtgacctgct ccgcatttcg tgtaatgttc ttcattattt cttaaagata aaataaactg
     7261 ccaatatcat gcatccgaat cccgctaaat aattccagcg aaaggtttca gtcttgaata
     7321 catataatgc catcaccgta aacacaacca gtgtgatcac ctcctgtaat attttgagtt
     7381 gaaataaatt gtatggtcct ccataagtgc tgctgccaat cctgttcgca ggaacctgaa
     7441 aacaatattc gaagaatgcc agtccccatg acagtaaaat aatcataaac aacccgctgt
     7501 tttcaaacca tcccagtttc ttgaattgta aatgaccata ccatgccagc gtcataaaaa
     7561 tattggagat gatgaggaag ataatagtgt atagtgattt caagttgttt gattttctga
     7621 atagctaaga tagggtgata gggtgagtgg gtgagtgggt cagtgggtga gtgttgtgtg
     7681 ggtgagcgga tttttggggc tggtattgat atttcatttc aggaactcaa aacatcgtta
     7741 gtttcaggcg tttgaagcga agatagatca attgaaattc ttgatgccgg attgttggag
     7801 gaagtcgtat ttatacttga cataaggcgt tacataggga tattcgaaac cttcgaaata
     7861 actccaatta taccagaatc cctgcatgcc tatccaggga aaataaactt cattatttgt
     7921 aaagctgata tcggtataaa atccgggtgc ccagcaatga acgatgtgat tgtacccatg
     7981 tttatcaacc acatacccca atccaacttt agcatcgtag taatcagtct ccatcatgaa
     8041 gttgatcgat ctgatatgat gccagcgatc ataatacgct tgcgtccata gcgaatgata
     8101 agacaacccg gtattgaatt cccggtcagc gctcatataa tctcgccata tcccaaaatc
     8161 aacatcatag ccgatcgcaa atccgccggc aaaggtatag ccgatggtga atgccggact
     8221 gacatagaaa ttatcacttg attgcgcaac accacgggca gcattgattt cgaggatcag
     8281 aaacaaaatt gcagttcgca aaattttgac aatggacata ggggagattt ggattgagtg
     8341 ctttaaaata tgcagatgct ttgatcacca aaattccata ataggagagg agagaggaaa
     8401 aaggagaaag gagaaaggtg aaagtagaaa ggtaaaagga gaaaggtgaa agaagaaagt
     8461 agaaagctga aaggaatggg atttattgtc tgtggggggt gatgaatagg tgctaacttt
     8521 gcttttcccc gcgtgacttc cacatctcat cgcgcaggca tcaatcctcc aatgtgaatt
     8581 taacgggcag gcgataccaa accgcaacat tttttccatc catcataccc ggtgtccatt
     8641 taggactgtt catgatcact cttaatgctt caaattccat tggcgggaat atcggtcgca
     8701 atgattctgc tttgataaca gcgccatctt ctgtaactac aaatttcatg tatactgtcc
     8761 cttcaacacc attatccctc gcggcgcgtg ggtattgaat atgaccactg atatatgcat
     8821 acaatcctga aacgcctccc ggaaactccg gcattttttc tgtaaattcc tgaatccctt
     8881 cctttgccgc aggtgcctcc tctgcgggct tctcaccgat cagaaccgtt tcattaccat
     8941 tttcatcaaa agttgattga tatataactt ctccattttc atgatacgaa atcttggatt
     9001 tatagtcgcc aaaagatgaa aatgtttcga tcaatctcag tttacctgtt ggataataat
     9061 aatagattga atcagtcagc ttatcattca caacatgtgt tttggactcc agtgcaccgt
     9121 cttcgtaact atatagatag ttcacttcat attcatacga tgaggtcatt tgctccaaag
     9181 agtcgtaagc gtattcacgg atcaatgtac cactggcatc aaaatatgat cttctgacct
     9241 tagtgccatc cggacgattg taaacgatag agtctcttcg taatccttta tccaggttga
     9301 ggtaactata ttgctgaagc aggatgccgt cggcagtagt aactttgata tcagcccaat
     9361 gctccttttc tgaatcaggg gtcgtgatta tagcgataaa tccttcatcc ggtttagttg
     9421 gcacttgttc gtaacgcaag ttgtagtaag ttgttgttgt aacgccttgt gcgtgtaagg
     9481 cgaacgacga aatgcagagc aatgcgatta ctaaggtctt cattggagat gatatttgat
     9541 ttgctaaaat aataaaatgt aatgaatgca ggttgcacgc aaaggcgcta agttgcaaag
     9601 gtttacacta attaattcaa cccacgaaaa tcaacaggca ctactttcga aatacccatt
     9661 tccggcatgg ttacaccata gataatatcc acttcagaca tggtacgctt gttgtgcgta
     9721 acgatgatga actgcgattt atcggcaaat ttgcggatgg tattgttgaa tttcatgatg
     9781 ttgttatcat ccaacggtgc atcaacctca tcgagaatac agaatggtgc aggtttaatc
     9841 agatacagtg caaaaagcaa actcatacct gtaagcgtct tctctccacc cgacaactga
     9901 tctatcacct gtggtttttt acccttaggt ttcgcgataa tctcaatttg cgcttctagc
     9961 ggatcgtaat catctttcaa taacaagtcg caatcatctt cttcattaaa taaactgcgg
    10021 aataccatct tgaagttttc acgaatttgc atgaaggctt cgtcaaattt attgcgcgca
    10081 gtctcatcaa tttctttaat agtatctaac agcgatgttt tcgcctgaag taaatcttct
    10141 cgctgtgttt taatgaaatc gtaacgcgta ttcatttcct cataggcctg aatcgccatc
    10201 ggattcacct cgccgtaatt ttcgatgcgc tttttcgcgg catctacttt tacttgtaat
    10261 tcttcaagcg taacatccgt aggtggttca ttatccaaaa tactttccaa ctcaacctgg
    10321 aattcaaccc ataatctttc tttgataccg gcaagttgca ctttcaattc gttcagcctg
    10381 tctttgattg aaccgagtgc atgttcactg ctatcttttt tcttctgcag tgctttgatc
    10441 ttgtcttcag tctcagctac ttcaccgcga ctgccataat aattttcctc ctcgcgactc
    10501 accatttgtt ccatggtagt tttttcgtcg taaagtttga ggagtgcttc ctgcatcacc
    10561 gcaactttct ctatgttctc ggcaatttct tctgtaacat cttgtaattg ctttttgctg
    10621 ctttcagccg tgatcatcgc ttcatcgcga cgagtacgct tgtaatcaag gtcctgctga
    10681 acactctgca atctgttgaa ctgctggtga tatttaatat tttcctggtt gtattgattg
    10741 ctgtggaaag tgagtttttc tgcaacctcg cgatacaata cctccatgtc gagcaactct
    10801 gcctggcgct tttctttcag ctccatgtgc ttgtcgtgcg actcatttaa tttgatgctc
    10861 tcttcatcag tttcttgtat ctgacgcatg aaagcaactt tcttatcctc catctgttgc
    10921 aataactgct cgatagattc aatgcgtgct tttgtggaaa cttgttgttg aacaacttta
    10981 tttaattctt cacgagcctg ttgtatctga cgatcaactg ccgattgttg taattctgat
    11041 aatttggttt gttgttcctt cagcgcatta ctttgtaatg tttcttcttc ctgtaaggtt
    11101 ttaatttccg cttccagcgt ttcgagtgtt ttctcgcgac caatacgctt gcctttaaat
    11161 aaaccaacac tacctccgga aatagcttta cctgaacgcg ctacgcgacc actctgatgc
    11221 aataagtgca catcaggatg agcagaggcg atgcgtgcac ttacggcagc aaaatcttca
    11281 tccgcagcaa taatgtatac attccccaat aaatgcgatg ccagtgcagc gtatttttca
    11341 tttatggtaa caacatccaa agcaggtacc acgccactga tggctgcagg ttgttgctta
    11401 gatttgaatt cacttaacag gaagaaattc gcacgtcctt tttcttttgt cgcgagttca
    11461 tttactgctt ccatggcatg agccatgtcc tgaacgatgt aataattcaa ccatggatcg
    11521 agaaaacttt caatggctgt tttgtatgca tcctgactgc tcaccacatc gctcagcaat
    11581 ggcgattcct tcatgctggc aacattcttc ttgaggtatt tgatcgattc agggaatcct
    11641 tcaaaactct cgaacatatt tttggtgagc gtaaattcat ttttcttcgc atccagtttg
    11701 cgacgtgcat tacctacctc attgcgcgat ttttctatct cgcgttgcaa tgctgcaatg
    11761 gtttgtttta aatcctgatc ttccttcatc aaggcatcaa cggtctgttg agcagcagca
    11821 cgattttcat catgcgcaac caattcctga cgcaatggat ccaatttatc cccttgtgtg
    11881 ccgcgatcac tttcaatatt gcctaactcg cgattgtaat tttcaagacg cactttgttg
    11941 atggcaatct ttttatcgat atcgtacaat tcgcgttcag cttgcttaaa tacggattga
    12001 gttgactcca gtttttcacg cacctgatcg tgatcttgtt tcactttttc aagcgactca
    12061 aggaaatgtt gaagcgattg tttgatgccg acgagtttct cgttttcttc ggcaacacgt
    12121 tgttccaatg aggcaatatc gccttgtgtt gatgcgatat attgctcagc atcttcaata
    12181 ctcttttgca ggcttacttg tttctcttca agaaatttcg tgcgctcctt gctgaggttg
    12241 gtatcgtttt ctttttgatg gatttcattc acaacagcat tgagatcctt ctgcaattta
    12301 ctcagcgatt gctctttatc gatgagggca gtttttttat ccgcaatgct tgcttccaac
    12361 gcatcgatct ctgcttcaat agaaagtttt gcatcacttt caaatgtctg ctgattactc
    12421 agttgcttat aaatatcctt atgcgattgc agggtgagca cagcaagatc gatgctgttt
    12481 aatttatatt caccctttaa tttctgatat cgttgggcgc gttttgcttg tttttcgagt
    12541 tctttgagat tgttttcgat ttcgaataac aggtcttcca cgcgatcaag atccagttgc
    12601 gtggcaccga gtttattcag tgtttctttt ttgcgcgatt tatattttga gataccggca
    12661 gcctgttcaa ataatttgcg acgtgaatta tccttatcgt gcaggatgtc atccaacata
    12721 cccaattcaa taatagaata agagtcggtg ctgattccgg tgtccatgaa gaggctttga
    12781 atatccttca ggcgacaagt aacaccgttg atgcgatact cgctctcacc ggttttaaag
    12841 tagtggcgtg atacggttac cgtgctgaat tcggttgcca gcaggttgcg tgtattctcg
    12901 aaggtgaggc tcacctcagc catacctgac gatttcctgt tcttcgtgcc gttgaagatc
    12961 aggttctcca tgcgctccgt tctcagtgca gtcggcttct gctcgccaag cacccagcgg
    13021 attgcatcca ccacattcga tttaccacag ccgtttggcc ctaccacccc ggtgatgttc
    13081 tcgttgaagt gcagaacagt cttgtctgca aaactcttga accctttgat ctctaacgtt
    13141 gaaagtttca tagtctttaa gcgggtcaaa aataaatgaa cgcagcaccc acgtcaaaaa
    13201 tgtgccgata tttgtattaa caattgtgga taaaatggat ttgataaagg tgtatgcctt
    13261 tcaaaacctg tgcaaatgca tccccaaaac agtattgaaa attggaaata atacggtctg
    13321 ctaaacacaa ttattcatac aagaattgca ctgtggaacg agaccgttgt tcgagataga
    13381 cttttttgtc gcccatcaat ttcttcaaca ggtcttcatt atagtgcctt accgtaagta
    13441 actgaagatc agtgttttcg cgcacgccaa ccttgctata cgaagccttc aggtcggcca
    13501 tgagtgattc caacaggtac gcatcgtaat ccaccacaat tgatacgctc tgtgcggcaa
    13561 tctgtatggt attgatgcgg agtttatgcc tggagaaaat ggcatagata tttgccatct
    13621 gcatatcgcc gacaaatgtg gtgagcgaag gggtgatgga gatgagcgtt tgtccgcttt
    13681 tatgcatgat cactgccggg taggggatga acttgttatc agtggcgatg accgtgcccg
    13741 attcctccgg atgcaggaac gaccgcacat aaagcggtat ctgcttctgt tggagtgggt
    13801 aaatggtctt tgggtggatg acactggcac cgtagtaggt catctctatt gcctcataat
    13861 aggtcagttc cggcaacagg accgcgtcgg gatacttttt cgggtctgca ctcagcactc
    13921 cgggaacgtc tttccatatc cattggcctt cggcatttgt acaatgggcg aggatagctg
    13981 ctgtgtagtc agagccttct ctgcccaaag tggtagtaaa accgtccgga gcgcctccaa
    14041 tgaagccctg ggtcactaca tgaccgcttt ttagcgccgc cgggagggta ttttgcactg
    14101 ctttaagcgt ttcctcccag ttcacctgcc cttcgcgcca ggaattgtcg gttttgatga
    14161 tttcgcgtgc atcaacccag gtattggcga tgccggccga caagaggtat tgactcaaaa
    14221 ggctgctcga catcagctcg ccgatactca caatctggtc gtagaactgc gggtaggagc
    14281 ggtgactgtt ttgccccgct agcgtaacca gcatatcacc aaagaagtcg aatccgttct
    14341 gcagtcccag ttctgctgca atgccggtat gctgcgcgat gaggctttga agtgcttcct
    14401 ggtagtcgcc accctcgtaa gcgcttacag ctatcttttc cagcgcattg gtcgtttttc
    14461 cgatcgccga caccacgata atgatatcct tatcctgaaa actgctgata atggttccca
    14521 cattccgcaa ccctgcagca tccttcaccg aggccccacc gaatttgaac actttcatgg
    14581 tgtaaaggta aaaagcaaaa gtgatagggg gttgaagagt agaacagccg tgtataggtt
    14641 ggggggaaat tagtatttca gaggaataag cttaattgtg acataacaaa ccacctcgaa
    14701 ttgcatcata aacggccgga ggagaggtgg aatggggggg tgtggttttg attggggatg
    14761 tttttgtgac atggagggat taacggtttt ccgtgaataa aaaaacatgt gggaagtgta
    14821 aatttgtaaa aggtaatccg cataaaaatt aacatttgtt tcgctttagc atctgttttg
    14881 gggtggttgg cgaaatgggg ttgcggctat aagtaagtta caagcaagcc tgagaaaaaa
    14941 gacagcgaca tgaaaattga caagacgaca tacaaagaca aagaagcaat tcaaatcgga
    15001 ctgactgccg accacaccag tcgacccgat aaagttttat tttttgccga cccgcatttt
    15061 ttgtttttta agagccgacc cacattgcac acattggcaa gtcgcacgag ccgacacaca
    15121 gaccaaaact tgccaaagag tgcaattttg ccgaccctcg catcgaaatt tgttagaata
    15181 attattaatt tgcactaaaa ta
//
LOCUS       DAOQHY010000077        15133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1877_length_15133_cov_6.170049, whole genome shotgun sequence.
ACCESSION   DAOQHY010000077 DAOQHY010000000
VERSION     DAOQHY010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 15133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15133
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1877_length_15133_cov_6.170049"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            401..2893
                     /locus_tag="PLL28_07845"
     CDS             401..2893
                     /locus_tag="PLL28_07845"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNF69275.1"
                     /translation="MGWRTGSAQLDQPNFIFIVVDDLNDYVQGITDQPQVLTPNIQEL
                     ANRGMLFSNAYANSPGCAPSRTSFLSGKDIQYTGVYNNEDYASKFRENFSVANGNEIV
                     YSLPQILKDSAGYFTYGINKIFHNPNNNDFDKTIATPACDKTLSWNRMDFIEDSDSLL
                     EALSVYGFGNTFDWGMIPDSLEPLLEDYIGTNLAINFIDSVATGTAETCGAPFFMALG
                     YYKPHIERFIPEKYFPDYYLNDVYAEPFVLPYNNPVDAYPYNGIVMPVQPDLLYGDYY
                     NLPEGGIARAMADNGGVYDQIETYVTGLDPLPVIDDLLTDEDRMNILRTATAANYEIN
                     YIAAIQYIDAQLGRLMDALEAYPAIYDNTIVVLLSDNGYSLGEKRHWTKWTLWEPDLR
                     VPMIIADPSREGGEVVEQTVSLLDLFPTVCDFAHVNYPTFPDGSKYLDGHSIAMLLDE
                     PNLKYEYPSLSSYKKNGGIGSCYPHHSVRNERWHYIRYRENNDGTFATGFCDSINHSY
                     QQELYEIGIDRETDPYEWNNLAEDPDYAPVINFLEQWMPDSVYYLKKAFRVTMQHDAI
                     DCLLDPEGSVHLSASVFDTAGILFDLPDTMQLRWYTNKSPEYTYGQVVDIDLTTIPDG
                     AFDIDAELIIYVELIGADSNIIQGFDLQYFYADPIHAPEATYDVAYIDALTIAVTGYT
                     LTGTYYTSWWDFGDGTTSPATDPITHVYPDEGPYTLTNFVSYGNDTCVAAFSKEISFT
                     TDTSDPEIQLFCYPNPVDDLLRIYLPVQSAYTAVDVFDLAGRPVLSQRFITANTLLFI
                     SLDCSKLNNGMYIVCLNTDQGRYSAPVVVLHP"
     gene            2980..4008
                     /locus_tag="PLL28_07850"
     CDS             2980..4008
                     /locus_tag="PLL28_07850"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNF69276.1"
                     /translation="MKRILATLLLAGSVLVASAQDIHFSQYSQSPLTLNPALTGLTPC
                     TYRGVLNYRNQWASALGPASYQTFAGSFDAGLWRDKFDGNIVGVGAMMYNDVSGDGSL
                     TNMTVMGSFAYHQMIGDENNYLSIGAQLGLVQKRVDYSKLIFESQIGVNGVDPNLPSG
                     EYGDDNFSYLDVNAGINWRGRFSDKFAVQIGGAYFHLSEPIETFYNYNVNKLNAHYVG
                     YGSFKIGMGKSTVLIPSFLYMQQTEGSNVQINSGVDVGFQLEKGVSMAYAGLHYRSVD
                     NIDVADAAIINLGLDYNNINFGLSYDVNISGLNAVTNYRGGIELSFVYWGCLSVTPKS
                     KPIDCPRF"
     gene            complement(4009..4749)
                     /locus_tag="PLL28_07855"
     CDS             complement(4009..4749)
                     /locus_tag="PLL28_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNF69277.1"
                     /translation="MDASATVFDNIVQSRRSMRIYDAEAYFDSEAVARSIRRAMLAPN
                     SSNMQMWEFYRVTDPQKIKDIAVFCMNQNAAKTARELLIIAVPKNKYRARAQANYAFH
                     HARYGNKPIEELDKRSARKLKYFSRLMPMYYFQDWLGLWGAVRKLIVSIASIWRPVVW
                     QVSKQDLRVVCHKSAALAAQTFMLSMLAEGYATCPMEGFDSKRLRKYLKLPRSTEFNM
                     IISCGPGTTQGIYHERFRVPENEVVFMV"
     gene            complement(4797..6773)
                     /locus_tag="PLL28_07860"
     CDS             complement(4797..6773)
                     /locus_tag="PLL28_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNF69278.1"
                     /translation="MLLILFQNHTHAQVNSSFELYRSNIAAAQANMQLHQFTEARNWL
                     NAAPEKYRAWEWNYLYYTLDQSIGKIELGYIANDMAISPDGKSIAIAGADHNIHIYDV
                     LSKSETSTIATHQGKVYSIAFDPSSQIIYSSGQDAKVIATKITDKSKVWEKSIPAGIN
                     KLSVSRDGKLICVSTTLFNGYGYTGNTIVLQAQTGSDIWKSGERKEKPLASAFSNDGS
                     MFAYGEENGVISVIRTDSFTVVQNYIFDDNNSIQQIKDITFSEDGSFLMAANGNGFAQ
                     VWNMFTRTTQQKLYSAQQNITTVGYTPNGFGMVTGASDGSIALWSTESATIKQELFGH
                     IGEVKKLLTTQNGDVLVSLSADGTLRFWDLAKAGTYINPMGFSGLTYAFEVSADSKYL
                     FTQGEFGLITQWNNATGYIEKQFPSLPEIMNDITIDHDNHLIAACDNEGHVRAWETAT
                     GKRYDDYFGVEGAVYDLDYNPDGTLLAAASADSNITIWNAVSGGIFQQIHFAGQVMDL
                     SFSYNGKMIAGVSSLGIIGVWNMETKEFVFNTTISKNHLQKVLFSPNGQNIIATDDNG
                     TIYILAADSGKLLKQLNAHTGIVYDVSFSVDGKRLASGGGDGTIKIWDTETWESVLSF
                     NDMHVWIHNVQFSSDGNHLYINAPTKDFKRYSIK"
     gene            complement(6870..9338)
                     /locus_tag="PLL28_07865"
     CDS             complement(6870..9338)
                     /locus_tag="PLL28_07865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNF69279.1"
                     /translation="MKKVILLLLFCQGVMLYAQQMQPKQNDYVSITNGKTKIPSKHIT
                     QELINNTPEAFQSHPDFGIPPVNAPKGAIELVQKRTLDSRYYIKEGTNGQSFFKQQAY
                     GAINYTDAQGYLRAIDYLLLPTINEGVFAAASQPTPTSIDFINNFTSIHIRDLDFRMN
                     HDMEMYFEKGNTTTAKQRYNTSNYTVGRDGTWITDIFSGVDGEIAFRKAGIKTNFIIK
                     SKEVVDPDAEWLVIDDHIALPPGYQFVYEDGDGYFLSNGDWKGNVVVKNPFGLNLLRI
                     ERPVILDQNKSKYHKQDQVDAIAYQLIPEDGGYILRMRVNVKWLINSERKYPVVIDPT
                     LIGEATYTAGDIGFEYDALCWNEADYCSYSLDITVPGKTTLTAAYFDGTYYSQNFGCF
                     FVTDCLMSEAAFRILGICDDSPGPASFWTCLPPVGDSAGTCYGIDLPMFNTIACIPPQ
                     CADYNFTFEMRTYHCSCTQPPCSILCHFMPTGSWVITIEGKTVEENPIQSSTVPDFVI
                     CEGDSIDLFASGVWGVPPYTYEWLPGGVVEPIHWVAPTTTTTYTSVIHDACDMTDTVS
                     QTVTVNPAPIIEVGPFEGCYLTTMTAPGGYDSYVWTDANDSVLATGTNTLTVDSTGMY
                     FVTVTDGNGCMGTSEPIEAFVYEAPVINAFPDTVFVNDGALALLQVETITGDDVTYTW
                     TPADAVNCLNCATTLAYSVGESNTFYVTGEEHGCVSAPDSIVVIMEESELIIPNAFTP
                     NDDGLNDEFNILNPIFYPIFSFEIYNRWGQQVFATSDVTRGWDGNYDGKPQEIGMYVW
                     MVSYEKANQPGEVFVLRGTVTLLR"
     gene            complement(9369..12290)
                     /locus_tag="PLL28_07870"
     CDS             complement(9369..12290)
                     /locus_tag="PLL28_07870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNF69280.1"
                     /translation="MTKLYTLIVGLIMSATLAGQTFQLFTEDFNVPTGSFILNTPGPG
                     GAAVGTNKWVINNSYTGGFGYPNTTTQDLTESGTIGGAPTSTYLHIYDEEGAPAITCA
                     SYDPTSTSDNFAEMSGGFCTLGLIDIEFTFFWLCEGGAGDYGQVYYSADGGPWTPVGM
                     ALYSGQTLWKYEVITDPAFEEVANLRFAFRWVNDNGGGPSTMSFAVDDIIAVGTYDEA
                     IHPVDINIDFISPDPVCKLSTLIIGWSLTDPLCDGIYEIELSNAAGIFAAPTSLGVFT
                     IFAEDTIGAIAAVIPGGVPDGSCYKVRLSRTSPLPEITGEASECFTIQSCPNIITTLQ
                     PVVTYDSNAVCVNSVIDVPFFSTGVYFPGNVYTAQLSDSTGSFDTYSVIGTFASNATY
                     DPALGSPPGSVSGIVPMVPPGCNYYIRVVSSTPASIGSAYGTVCIQECDIETNDIEDV
                     YACITEDSGVTITITYDVNVFDDIETYCDTNTFCVEVLNSMFFTQANLCGLGFTIDTE
                     SGTIDLEIPGYFDLVAMGLDAGMWYMRVIANCGTPQENTLGTLVHLTIGAPADNAPVL
                     IPSDTLLCEGAVGNATIVPYNMNSDYQFQFGAGTPFIWPFNPIFIDFTGATGDVTLRV
                     REINYNCPGPWSEYVTFHIIDAPIVTISGPPKACTGDTTWYSVPYFLTTYYDWSLTGG
                     TIVDTSNNVVGIIWDEAGTYTLSIFALNECGSGNGTKNINVIETVPVEQSEDKTICEG
                     SSATFSVLTVGVPYYEWSDAETGEVIGDDFIITVTPDSTSTYVVTAEDEEGCNSYDTV
                     TVFVEYPSTESDTASYCIGGSVIIDAGHEGATYLWNTGETTKTIEVNAAGTYIVTINA
                     PDLACDITKSIDVSEVIDNCAPIVNIPNAFSPNGDGVNDFLTIYGAAVIDINIHIYNR
                     WGELVYTTNDLGVLNSGSLGWDGKANGQDQEMGTYVYVVSATGGNGNTITLQGNVTLV
                     R"
     gene            12417..13904
                     /locus_tag="PLL28_07875"
     CDS             12417..13904
                     /locus_tag="PLL28_07875"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HNF69281.1"
                     /translation="MRALNRLATQTAIYGMSTILVRLLNYVLAPLHTRVFTNQADYGI
                     ISEMYAYVTFLNVVCMFGLETAFFRFASNNNQNSLDKRSVFGSSQISLIVSTALITAA
                     LLFSSNDIASMLGYPGKGIYVTFFALIIAFDTLVNIPFARLRLEGRPWRYFAIKLTNI
                     IINVSFNFFFLLPALRNNYDMFSGIGYTYDPDYGVAYVFLANLIASAITFAIFIPGML
                     SLKFDVQVWKKLMAYGLPLIIIGLAGMINETLDRVLIKYWGSGTIEENLKQVGIYSAV
                     YKLSIFMTLAVQGFRMGAEPFFFSQSAEKNAPSLYALIMRYFVIACCLIFLGVGMFPD
                     LFKIIIGSEYHSGLHIVPILLLAQLFLGIYYNQSVWYKLTDKTSFATIIPVAGALITL
                     GMNLILIPKFGYTGAAWSTFACYLSMVVMSHIIGQKHYHVPYNLRKIITYIVVSVILC
                     YSGLLLMSATENIVVTIIVRSCLFGLFVAFAWWLDLSKLLRRNKS"
     gene            13904..14341
                     /gene="dut"
                     /locus_tag="PLL28_07880"
     CDS             13904..14341
                     /gene="dut"
                     /locus_tag="PLL28_07880"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNF69282.1"
                     /translation="MLFMLIKIVNTSNNPDPTYATAHAAGMDIRAFVDAPVTILPMQR
                     TLIPTGLFIELPEGYEAQIRPRSGLAINSGITMLNTPGTIDADYRGEIKVIVINLGDA
                     PFIVNSGDRIAQMVIAKYESVTWVNTDKLSESERGAGGFGHTG"
     gene            14414..>15133
                     /locus_tag="PLL28_07885"
     CDS             14414..>15133
                     /locus_tag="PLL28_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNF69283.1"
                     /translation="MNIIIPMAGMGKRMRPHTLTVPKPLIPVAGKPIVQRLVEDIAHT
                     TDEKIDNIAFVVGRFGKEVEDMLVGIAAKLGAKGHICYQDEPLGTAHAIMCAKDYVTG
                     PTIIAFADTLFKTNLKIDTTIDGIIWVNKVENWKMFGVVEMNADGYITNFVEKPDHYV
                     SDLAIIGVYYVKDGDLLRNEMQYLLDNNIREKGEYQLTNALESMKQKGYRFSPGKVDE
                     WLDCGNKDATVYTNQRVLELKK"
BASE COUNT         4297 a         3196 c         3074 g         4566 t
ORIGIN      
        1 agtagtgatg ataacccaga ttgtcgaaaa tgctcaaatc caactgtccg ggctcggctt
       61 tcagttcctg gaggcaaatg atgtcaaatc cctccttctg cacccattcg gcccatccct
      121 ttgtcagcgc cgctctgata ccattcacgt tgtaggtaat gattttcatg atcatagtta
      181 tcgtccaaag gtaatattgc caattgattg ataataactg caagcgctcg agtttgcagc
      241 ttttcgtaac tttaacttcc gtttgctttg caaaataact tgttaaaata ttatttcaac
      301 gcccatttta tgggaaactt atcggaagct cccctaaaag ttcaaaatac ccatgcataa
      361 aacgttaaaa tcttttttca catgttttct ctttttgctc ttgggttggc gaacgggatc
      421 tgcacaactc gatcagccaa attttatttt tatagttgtt gatgatttga atgattatgt
      481 gcaaggtatt accgatcagc cacaggtgct cacgccaaat attcaggagc ttgcgaatag
      541 aggcatgctt ttttcaaatg catatgctaa ttcgccggga tgcgctcctt cgcgaacaag
      601 ttttttaagc gggaaggata tccaatatac aggtgtatat aataatgagg attatgcctc
      661 taaattcaga gagaattttt ctgttgccaa tggaaatgaa atagtttatt cgctgcctca
      721 gatattaaaa gatagtgcag gatattttac ttatggaatc aataaaattt tccataatcc
      781 gaataataac gattttgata aaactatagc aacgccggca tgtgataaga ccttgtcgtg
      841 gaatcgcatg gattttattg aagattccga ttccttgctg gaagcattat ctgtttatgg
      901 ctttggcaat acatttgatt ggggtatgat accagatagc cttgaacctt tactcgaaga
      961 ctatatcgga acaaatcttg ctattaactt cattgattcc gtagcaaccg gaactgcaga
     1021 aacctgcggc gctccttttt ttatggcttt gggatattat aagccacaca tcgagcggtt
     1081 tatcccggaa aaatattttc ctgattatta tttaaatgat gtgtatgccg agccctttgt
     1141 tttaccttat aataatcctg tagatgccta tccatacaac ggaattgtaa tgccggttca
     1201 acccgatttg ttatacggcg attactataa tttaccggaa ggtggaattg cacgtgcaat
     1261 ggctgataat ggcggagtct atgatcagat agaaacctat gttacaggtc tggatcctct
     1321 ccctgttatc gatgacctgc tgacagatga ggatagaatg aatatcctgc gcactgcaac
     1381 agctgccaat tatgaaataa attatatcgc tgcaattcaa tatatcgatg cacaacttgg
     1441 cagattgatg gatgcattgg aggcttatcc tgcaatatat gataatacta tcgtggtatt
     1501 attaagcgat aatggctatt ctctcggcga aaaacgccat tggacaaaat ggacattatg
     1561 ggaaccggat cttcgtgtgc caatgatcat tgccgaccct agtcgcgaag gtggcgaagt
     1621 ggttgaacag acggtttcat tattagactt atttcctaca gtatgcgatt ttgcgcatgt
     1681 gaactaccct acttttcctg atggttctaa atatctcgat gggcatagta ttgctatgtt
     1741 gctcgatgaa ccaaatctta aatatgagta tccaagttta tcgtcctata aaaagaacgg
     1801 cggtatcggt tcatgctatc cgcatcattc tgtaagaaat gaacgctggc attatatccg
     1861 atatcgtgaa aataatgatg gcacttttgc caccggattc tgtgattcga taaatcacag
     1921 ctatcaacag gaattatatg aaataggtat cgacagagaa acagatccct atgaatggaa
     1981 taatctggca gaagatcctg attatgcacc ggtaatcaat tttctggagc agtggatgcc
     2041 ggatagtgtt tattatttaa aaaaggcttt cagagtaacc atgcagcatg atgctatcga
     2101 ctgtcttctc gaccctgaag gctcagtgca tctttctgct tctgtgtttg atacggcagg
     2161 aattttattt gatcttcccg atactatgca gttgagatgg tataccaata aatcgcctga
     2221 atatacttac ggtcaggtag tggatattga tttaactaca attccggatg gtgcttttga
     2281 tatcgatgcc gagctcatca tttatgttga actgataggt gccgatagta atatcattca
     2341 aggtttcgac ctgcaatatt tctatgccga tcctatacat gctcctgagg caacttatga
     2401 tgttgcctat attgatgctt tgacaattgc cgtaacaggc tataccctta caggaacata
     2461 ttatacttct tggtgggatt ttggcgatgg cactacttcg ccggcaacgg accctatcac
     2521 ccacgtttac cccgatgaag ggccgtatac cctcaccaat ttcgtgagtt atggcaatga
     2581 cacctgtgtt gctgcattca gcaaggagat atccttcaca acagatactt cagaccctga
     2641 aattcaacta ttctgctatc caaatccggt tgatgatttg ctaaggatct accttccggt
     2701 gcaatctgca tacacggctg tagacgtatt tgacctggcc gggcgacctg tgctttcgca
     2761 acggtttatt acagctaata ccttgttatt catatcgttg gattgcagta agctcaacaa
     2821 tgggatgtac attgtatgcc tgaataccga tcagggaagg tactccgccc ctgtagttgt
     2881 attgcatcca tgacagaata gttacataga tgtattgtta atgttgtgtg gaatactaaa
     2941 gtttgctact tttgggttaa gttagccgaa atccaacgta tgaaaagaat cctcgcaacc
     3001 ctcctcctcg ccggttccgt actggttgcc tcagcgcagg atatccattt ttcgcagtat
     3061 tctcagtcac ctttgacctt gaaccctgcg ttaaccggac ttactccctg tacttataga
     3121 ggtgtgctca attatcgtaa ccagtgggct tctgcattgg gacctgcaag ttatcagacc
     3181 tttgccggat cttttgatgc cggtttgtgg cgcgataagt tcgatggaaa tattgttggg
     3241 gttggtgcaa tgatgtacaa tgatgtgtca ggagatggtt cactgactaa catgaccgtt
     3301 atgggttcat ttgcatatca tcaaatgatt ggagatgaaa acaattatct gtctatcggt
     3361 gcgcaacttg gattagtgca aaaacgtgta gactactcaa aactcatttt cgaatcgcaa
     3421 attggagtta atggtgttga tccgaatctt ccaagtggcg aatatggtga tgataatttc
     3481 tcatatcttg atgtaaatgc gggtattaac tggagaggtc gtttttcaga taaattcgct
     3541 gttcaaatcg gaggtgccta tttccactta agtgaaccta ttgaaacatt ctacaactac
     3601 aatgtaaata agctgaatgc gcattacgtg ggatatggat ctttcaaaat cggtatggga
     3661 aaaagtacag tattgattcc ttcattcctt tatatgcaac agactgaagg gtcaaacgtt
     3721 caaataaact caggtgttga tgtcggcttc caactcgaaa aaggtgtttc tatggcttat
     3781 gccggattgc actatcgttc agtggataat atcgatgttg ctgatgcggc cattattaac
     3841 ctcggattag attataataa catcaatttc ggtttaagct acgatgttaa tatttccgga
     3901 ttaaatgccg taaccaatta tcgcggtggt atcgagttgt cgttcgtgta ttggggttgc
     3961 ctcagcgtaa caccaaaatc gaaacctatc gattgtccac gattctgatc aaaccataaa
     4021 tactacttca ttttccggta cacgaaatct ttcgtggtaa ataccttgtg ttgtaccggg
     4081 tccgcaactg atgatcatat tgaactcagt gcttcttggc agtttaaggt atttgcgcaa
     4141 gcgctttgaa tcaaaacctt ccatcggaca tgtggcatat ccttctgcta acatgctaag
     4201 cataaatgtt tgtgcagcaa gtgcagctga tttgtgacat actactctta aatcctgctt
     4261 gcttacctgc caaactaccg gacgccaaat ggaggcaatt gatactatta atttgcgaac
     4321 tgcaccccat aaaccgagcc aatcctggaa ataatacatc ggcatcaacc gcgaaaagta
     4381 cttcagcttt cttgctgaac gcttatccaa ctcttcgatt ggtttattac catatcgtgc
     4441 gtgatgaaat gcataattcg cttgagcacg cgctctgtat ttatttttcg gcacagcaat
     4501 aatcaataat tctcgagcag tttttgccgc attctgattc atgcaaaata ctgcaatgtc
     4561 ttttattttt tgcggatcgg taacgcgata aaattcccac atctgcatgt tgctgctatt
     4621 aggcgcaagc attgctctgc gtatacttcg tgcaaccgcc tcactatcaa agtaagcctc
     4681 ggcatcataa atgcgcatac tgcgacgaga ttgcactata ttatcaaaaa ctgttgcaga
     4741 tgcatccatt gtaattcgtt aattgtagaa gaaaattatt atttaatgtc gagatattac
     4801 ttaatgctat agcgcttgaa atctttggtc ggggcattaa tgtataaatg atttccatcg
     4861 gaactgaatt gaacattgtg aatccagaca tgcatgtcat taaaagataa aacagattcc
     4921 catgtttcag tgtcccagat tttaatagtg ccatcgccac cacctgaagc gaggcgcttt
     4981 ccatcaaccg aaaacgaaac gtcgtaaaca atgcctgtat gcgcgttcag ctgtttcaaa
     5041 agtttacctg aatcggcagc taaaatatat atcgttccat tgtcatctgt ggcaataata
     5101 ttttgaccat ttggtgaaaa aagtactttt tgtaaatgat ttttagaaat cgttgtgttg
     5161 aacacaaatt cttttgtttc catattccat acaccaatta ttcccaagga tgaaacccct
     5221 gcaatcattt ttccattgta gctgaaactt agatccatca cctgtccggc gaaatgaatt
     5281 tgctgaaaaa tgccgccgct gacagcattc caaatagtga tattactatc ggcactcgct
     5341 gcagctaata aagttccatc cggattataa tcaaggtcgt aaacagctcc ttctacgcca
     5401 aaataatcat catatcgttt acccgttgct gtttcccagg cgcgaacatg tccttcgttg
     5461 tcgcatgctg caattaaatg attgtcgtga tctattgtga tatcattcat tatttccggc
     5521 agcgatggga attgcttttc tatataacct gttgcattat tccattgcgt aatgagtccg
     5581 aattctccct gtgtaaataa atatttactg tcggcagaaa cttcaaaggc ataggtcaat
     5641 ccgctgaaac ccattgggtt aatataagtg cctgctttcg caagatccca aaaccttaat
     5701 gtgccatcgg cagacagtga aaccaataca tcaccgtttt gtgtagtgag cagttttttt
     5761 acttcgccga tatggccaaa taattcttgc ttgatagtgg cgctttctgt actccacaac
     5821 gcaatactac catccgaagc accggttacc atgccgaatc catttggtgt gtaacctacc
     5881 gtggtaatat tttgttgagc actatataat ttttgctgtg ttgttcgggt gaacatattc
     5941 caaacttgcg caaatccgtt tccattagcc gccattaaaa aggagccatc ttcactaaag
     6001 gtgatatctt taatttgttg aatggaatta ttatcatcga aaatgtaatt ctgcacaaca
     6061 gtaaaactat cagtgcgaat tacactgatt acgccgtttt cttctccata agcaaacatt
     6121 gagccatcat tgctaaatgc agaggcaaga ggtttttctt ttctttcgcc acttttccag
     6181 atatcacttc cggtttgtgc ctgtaacact atggtgtttc ctgtatagcc atatccattg
     6241 aatagggtag tgctgacaca aatcaattta ccatcccgac taacagataa tttatttatt
     6301 cctgccggaa ttgatttttc ccagaccttc gacttgtctg ttatttttgt tgctatcacc
     6361 tttgcatcct ggccggagga ataaattatt tgcgatgaag gatcaaaagc tatgctatat
     6421 accttgcctt gatgtgtagc aattgtagat gtttcagatt tactaaggac atcatatata
     6481 tgaatattat gatcagctcc ggcgatagca atagacttgc catcgggtga aattgccata
     6541 tcattagcaa tgtagccaag ttcaattttt cctatactct ggtcgagcgt gtagtaaaga
     6601 taattccatt cccaggctct gtatttttca ggcgctgcat tcaaccaatt tcgcgcttca
     6661 gtaaactgat gtaattgcat attcgcttgt gcagcggcaa tattggaacg atataattcg
     6721 aatgaactat taacctgtgc atgtgtatga ttctgaaaaa gtatcagaag caaaatgcag
     6781 taaataggag caattgcatg aattgaaatt cgcctcatca taatgatcct gcaaatttaa
     6841 tgcaaggaat taaggacatc tattgaaatt tagcgcagta aggtcacagt gccgcgcagc
     6901 acaaagactt caccgggttg attggctttt tcgtaactca ccatccacac atacatgcca
     6961 atttcctgag gtttgccgtc gtaatttcca tcccagccac gggtaacatc gctggtagca
     7021 aatacctgct gaccccagcg attgtagatt tcgaaagaga aaatcggata aaaaatggga
     7081 ttcaggatgt taaattcgtc gtttagtcca tcgtcattag gagtaaacgc attgggtatg
     7141 atcagttcac tttcttccat gattacgact atgctatccg gggctgaaac acagccatgt
     7201 tcttcgccgg ttacataaaa agtatttgac tccccaacac tgtaagcaag tgtggtggca
     7261 cagtttaaac aattaacagc atcggccggt gtccaggtat aagtgacgtc atcacctgta
     7321 atagtttcaa cctgaagaag agcaagtgcg ccatcattta caaaaacggt atcaggaaat
     7381 gcatttatca caggcgcctc ataaacaaat gcctcaatcg gttccgaggt acccatacat
     7441 ccatttccat cggttactgt aacaaaatac atgccggttg aatcaacagt gagcgtattc
     7501 gtacctgttg ctaatacact gtcatttgca tctgtccata cataactgtc atatcctccg
     7561 ggtgctgtca ttgttgtgag gtagcaacct tcaaatggtc caacttcaat aattggagcg
     7621 ggatttacgg ttactgtttg cgaaacggta tcggtcatat cgcatgcatc atggatcact
     7681 gatgtgtaag tggtggttgt agttggcgct acccaatgta ttggttctac aacacctccc
     7741 ggcaaccatt cgtaggtata tggtggaaca ccccagacgc cggatgcaaa taaatctata
     7801 gaatcgcctt cgcaaatcac aaaatcggga actgtagatg actgaatagg attttcttcc
     7861 actgtttttc cttcaatggt aatcacccag cttccggtag gcatgaagtg acataaaata
     7921 ctgcacggtg gttgtgtaca tgaacagtga taagtacgca tctcgaatgt gaaattataa
     7981 tcggcacatt gcggtggaat acaggctata gtattaaaca tgggcagatc aattccataa
     8041 caagttccgg cactatcgcc caccggcgga agacaagtcc agaatgaggc tggtcccggg
     8101 gagtcatcac atattcctaa aatgcgaaaa gcagcttcac tcattaaaca gtcggtgacg
     8161 aaaaaacatc cgaagttttg tgagtaataa gtgccatcaa aatatgctgc agtcaatgta
     8221 gtttttccgg gaacagtaat gtccagtgaa tagctgcaat agtcagcttc attccagcaa
     8281 agtgcatcat attcaaagcc aatatcaccg gcggtataag tagcctcgcc aattaatgtt
     8341 ggatcaataa caaccgggta ttttcgttct gaatttatta gccattttac attaacgcgc
     8401 atacgtaaaa tatatccgcc atcctccgga attaattggt atgctatagc atcaacctga
     8461 tcttgcttat gatatttact tttgttttga tcgagtatta caggacgttc gatgcgcagc
     8521 aaattcaatc cgaaaggatt tttcacaaca acatttcctt tccagtcgcc attggataaa
     8581 aaatatccgt caccatcttc gtaaacgaat tgataccccg ggggaagtgc aatatggtcg
     8641 tcaataacca gccactcagc atccgggtcg acgacctctt ttgatttgat gatgaaattt
     8701 gtttttattc cggcctttct gaaagcgatc tcaccatcaa ccccggagaa tatatcagtg
     8761 atccatgtgc catcgcgccc taccgtgtaa ttacttgtat tgtaacgttg ctttgcagta
     8821 gttgtatttc ctttttcaaa atacatttcc atatcatgat tcatacgaaa atcaagatct
     8881 cgaatatgta tagaggtgaa attgttgatg aaatcaattg aagtaggagt tggttgtgag
     8941 gcagcagcaa atacgccttc gttaattgta ggtagcaata aataatctat agcacgcaaa
     9001 tatccctgag catcggtgta attaattgcg ccgtatgcct gttgtttaaa aaatgattgt
     9061 ccgttggtgc cttcctttat ataataccgt gaatccagtg tgcgcttttg tacaagctct
     9121 atggctccct ttggtgcatt aacaggtggt atgccaaagt cgggatgcga ttgaaatgct
     9181 tcgggcgtgt tgttaatcag ctcctgcgta atatgttttg acggaatttt tgttttgcca
     9241 ttcgtgatcg acacatagtc attctgctta ggctgcattt gctgagcata cagcattacg
     9301 ccctgacaaa agaggagtaa tagaatgact tttttcatac ctgtatcaaa ctacactgca
     9361 aggcaatatc aacgcactaa cgtgacattc ccctgcaatg tgatcgtatt tccattgccg
     9421 ccggtagcgc ttaccacata aacataagtg cccatttcct gatcctgacc atttgcttta
     9481 ccatcccatc ccaatgaacc actgttgagc acgccaagat catttgttgt atatactaat
     9541 tcaccccagc gattataaat atgtatattg atatcaataa ctgctgcgcc gtagattgtc
     9601 agaaaatcat tcacaccatc accatttggc gagaaggcat tcggaatatt tacaataggg
     9661 gcacaattat caatcacttc acttacatca attgattttg taatgtcaca tgcaagatca
     9721 ggtgcattga ttgttacaat ataagttcct gcggcattaa cctctattgt ttttgtggtt
     9781 tcgcctgtat tccacaaata agtagcacct tcatgaccgg catcaatgat caccgaacca
     9841 ccaatacaat atgatgcagt atctgattct gttgatggat attctacaaa aacggttacg
     9901 gtatcataac tattacaacc ttcttcatct tcggcagtga ccacgtaagt acttgtggaa
     9961 tctggtgtta cagtgataat aaaatcatca ccgattactt caccggtttc tgcatcagac
    10021 cattcgtaat acggcacgcc aaccgtgagc acactaaagg tggcactgct tccttcacaa
    10081 atagttttgt cttccgattg ctctacagga acagtttcaa ttacattaat attttttgtt
    10141 ccattgccac tgccacattc gttcagtgca aaaatgctta atgtatacgt acccgcttca
    10201 tcccaaataa ttccaactac gttgttcgaa gtatcaacta ttgttcctcc ggttaatgac
    10261 cagtcgtaat atgttgtcag gaaatacgga acggaatacc aggttgtatc accggtacac
    10321 gctttcggag gacctgaaat ggtaactatt ggggcatcta taatatggaa agtaacgtat
    10381 tcactccatg gtcccggaca gttgtaattt atttcgcgaa cacgaagcgt aacatcacct
    10441 gttgcacctg tgaagtcaat aaatattggg ttgaaaggcc agataaatgg tgttcctgct
    10501 ccgaactgga attgataatc ggaattcata ttatagggaa cgatcgttgc attgccaaca
    10561 gcgccttcac acaataaggt atcggaagga attaaaaccg gagcattatc tgcaggtgca
    10621 ccaattgtta aatgaaccag tgtacctaat gtattttcct gcggtgttcc gcagtttgca
    10681 attacacgca tataccacat accggcgtcg agccccatcg ctacgagatc aaaatatccc
    10741 ggtatttcca aatcaattgt tcccgattct gtatcaatgg taaatcctaa tccacacaaa
    10801 ttagcctgag tgaaaaacat ggaatttaat acttccacgc agaaggtatt tgtatcgcaa
    10861 tacgtttcta tatcatcaaa cacattgaca tcataggtta tagttatagt aactccggaa
    10921 tcttctgtga tacatgcgta aacatcttca atatcatttg tttcgatatc acactcctga
    10981 atacagaccg ttccgtatgc cgaccctatg gatgctggtg tactcgagac gacacggatg
    11041 taatagttac atccaggcgg caccattggt acgatacctg acacagatcc cggtggtgag
    11101 ccgagggcag ggtcatacgt tgcgttgctt gcaaaggtgc ctatcacaga ataagtatcg
    11161 aagctgccgg ttgaatcact caactgcgca gtataaacat tccccgggaa gtatactccc
    11221 gttgagaaaa atggcacatc gatcacactg ttgacacaca ccgcattcga gtcgtatgtg
    11281 acgacaggtt gtaaggtggt gattatattc ggacaacttt gaatcgtgaa acattcactc
    11341 gcctcacctg taatttccgg taagggcgac gttcggctga gccttacctt atagcaggat
    11401 ccatcgggaa cacctccggg aatcacggct gcaatggcac caatcgtgtc ttcagcaaaa
    11461 atggtaaata cgcccaaact tgttggcgca gcaaaaatcc cggctgcatt tgacagttct
    11521 atctcataaa taccatcgca taatgggtca gtcagcgacc aaccaataat gagtgtgctc
    11581 aacttacata ccggatcagg tgaaataaag tcgatattga tatctactgg atgtatagcc
    11641 tcatcataag tacctactgc aatgatatca tctacggcaa atgacatcgt gcttggacca
    11701 ccgccattgt cgtttaccca tctgaatgca aaccggagat tggcaacttc ctcaaatgca
    11761 ggatcggtta taacttcata tttccagagg gtttgaccac tgtataacgc catccccacc
    11821 ggagtccaag ggccgccatc agcactataa tacacctgac catagtcgcc ggcaccaccc
    11881 tcgcatagcc agaaaaaagt gaattcgata tcgatgagac ccagtgtaca aaagcctcct
    11941 gacatttcag caaagttgtc agaggtactg gttgggtcat aactggcaca ggtaattgcc
    12001 ggagcaccct cctcatcata aatgtgcaaa taggtacttg taggtgcgcc acctatggtt
    12061 cctgattctg taaggtcttg agtggtagtg ttcggatacc caaatccacc ggtgtaactg
    12121 ttgttaatga cccatttatt ggttccaaca gccgcgccac ctggtccggg tgtgtttaaa
    12181 ataaaggaac cggtaggaac gttaaagtct tcagtaaata gctgaaaggt ctgtcccgca
    12241 agggttgcag acatgataag ccctactatg agtgtgtata atttggtcat aggcgaaatc
    12301 gaggactact aagataaacc ttattataga aaaacccaat tgaaccgcac ggtatcattg
    12361 agatcgtgta attatttgag actcaatcac tctttcaaac accttatctt cgccctgtgc
    12421 gcgcattgaa tcgtcttgcc acccaaaccg ccatctatgg catgagtacc attctggtgc
    12481 gattgctcaa ttatgtgctg gctcccttgc acacgcgcgt atttaccaat caggctgatt
    12541 acggtatcat ctctgaaatg tatgcatatg tcacattttt aaatgtggtt tgcatgtttg
    12601 gactcgaaac ggcttttttt cgtttcgcct caaataataa tcaaaattcg ctcgacaaaa
    12661 gaagtgtttt tggctcatcg caaatatcat tgatcgtttc tacagcatta ataacagctg
    12721 cactcttatt ttcaagtaat gatattgcct ccatgcttgg atatccggga aagggaattt
    12781 atgttacatt ttttgcactc attatagcat tcgatacatt agtgaatatt ccttttgccc
    12841 gtttgcgact ggaaggaagg ccctggcgat attttgcaat taaattgacg aatatcatta
    12901 tcaatgtttc attcaatttc tttttccttt taccggcact gagaaataat tatgacatgt
    12961 tttccggaat aggatacacc tatgatcctg attatggcgt tgcttatgta tttctagcca
    13021 atctaattgc tagtgctatc actttcgcca tatttattcc tggcatgttg tcattgaaat
    13081 tcgatgtaca agtatggaaa aaattgatgg cgtatggatt gcctttgatc atcatcggtc
    13141 ttgcgggaat gatcaatgaa actctagata gagtgctcat taaatattgg ggttcaggca
    13201 caatagaaga gaacctgaaa caagtgggta tatacagcgc cgtgtataag ttatcgatct
    13261 ttatgacgct tgcggtgcaa gggtttcgga tgggagcaga gccctttttc tttagccagt
    13321 cggcagaaaa aaatgcgcct tctttatatg cgttgatcat gcgctatttt gttattgcat
    13381 gctgcctgat atttttgggt gtaggaatgt ttcccgactt atttaaaatt atcatcggct
    13441 ccgaatatca ctccggatta cacatcgttc cgatattatt attggcacaa ctttttttgg
    13501 gaatttatta caatcaaagt gtatggtata agcttaccga taaaacgtct tttgcaacaa
    13561 taatccccgt agccggtgca ctcattacac tgggcatgaa tttaattctg atacccaaat
    13621 tcggatatac cggtgcagct tggtcaactt ttgcgtgtta tttaagtatg gtggtgatga
    13681 gtcatatcat tggacagaaa cattatcacg tgccctataa tttgcgaaaa ataattacct
    13741 acattgtagt ttctgttata ctgtgctact ctggattatt acttatgtcg gcaacagaaa
    13801 atattgtcgt caccattatt gtaagaagct gtttattcgg actatttgtc gcctttgcat
    13861 ggtggcttga tctgagtaaa cttttgagaa gaaataaatc ttaatattat tcatgttgat
    13921 taaaatcgtc aatacatcaa ataaccccga tccgacttat gccactgcgc acgctgccgg
    13981 tatggacata cgggcttttg tagatgcacc ggttaccata ctgcctatgc agcgcaccct
    14041 gattccaacc ggattattta tagagctgcc tgaaggatat gaggctcaaa tacgccccag
    14101 gagcggattg gctataaaca gtggcatcac tatgctcaat acccccggca ctattgatgc
    14161 tgattaccgt ggtgaaataa aggtgattgt aattaatctg ggagatgccc cttttatcgt
    14221 taattcgggc gataggatag ctcaaatggt cattgcgaaa tacgaatctg ttacctgggt
    14281 gaatacagac aagctttctg agtcggaacg cggtgcaggc gggtttggcc acaccggata
    14341 agtaaactcc ttgttgtcaa ttcagcatcc acatgattca tcattatctt taccactcaa
    14401 ttttaatagt acaatgaaca tcatcattcc aatggccggc atgggaaaac gcatgcgacc
    14461 acatacctta actgttccta aaccattgat tcctgtcgcc ggaaagccaa ttgtgcagcg
    14521 acttgtagaa gatatagccc acacaaccga cgaaaaaatc gacaacatag cctttgttgt
    14581 gggtcgattt ggcaaggaag ttgaggatat gctggtaggc atagctgcga aattgggtgc
    14641 aaagggtcat atctgttatc aggatgagcc tcttggaacg gcacatgcca ttatgtgtgc
    14701 taaagattat gtgacaggac ctaccatcat tgcatttgcc gataccttgt ttaaaacaaa
    14761 cctgaaaata gatactacca ttgacggtat catttgggtg aataaagtag aaaactggaa
    14821 aatgtttgga gtggttgaaa tgaatgccga tggttatatc accaattttg ttgaaaaacc
    14881 ggatcattac gtttccgatc tggcaatcat tggcgtgtat tatgtgaaag atggcgattt
    14941 gctgaggaat gaaatgcagt atctgctcga taataatatc cgtgaaaaag gagaatatca
    15001 gcttaccaat gctttggagt cgatgaaaca aaagggatat cgcttcagcc ccggaaaagt
    15061 agacgaatgg ctcgattgcg gaaataaaga tgctaccgtt tacaccaatc agcgtgtgct
    15121 ggagttgaaa aag
//
LOCUS       DAOQHY010000078        14879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1917_length_14879_cov_5.042094, whole genome shotgun sequence.
ACCESSION   DAOQHY010000078 DAOQHY010000000
VERSION     DAOQHY010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14879
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1917_length_14879_cov_5.042094"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /locus_tag="PLL28_07890"
     CDS             <1..1398
                     /locus_tag="PLL28_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNF69284.1"
                     /translation="YVTFIPDNTIFRNFHFIPEYLDNQLWNYVFLNSGLTINFNGQKY
                     YSQNGLHDLLVRNSNAETNRYPIIHLKGDDIEIALTHGNEYGEDYYSFVNGQNTTQGG
                     THLAAFREGLVKTVREFYKKDFDASDIRQAIVGAIAIRVQEPVFESQTKTKLGSIYTS
                     PDAQVTVKSFVVDFVKKELDNYLHKNPATADALLKRILQSERERKDIAGIKKLANERA
                     KKANLHNRKLRDCRYHLGEDKHEKTLRSTLFITEGDSASGSITKARDVESQAVFSLKG
                     KPLNVFGLTKKIVYENEEFNLLQHALNIEDGIDGLRYNQIVVATDADVDGMHIRLLLL
                     TFFLQFFPEVVKNGHLYILQTPLFRVRNKKETIYCYNEEEKQVAVNKLGNKPEITRFK
                     GLGEISPDEFSGFIGENIRLEPVIIGKEQSIQSTLEYYMGKNTPDRQTFIIRNLRVEK
                     DVIDEPAIVEAESEV"
     gene            complement(1395..3002)
                     /locus_tag="PLL28_07895"
     CDS             complement(1395..3002)
                     /locus_tag="PLL28_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69285.1"
                     /translation="MAHKKPQKPEDHPIDKLFRDSLENRDFPYDEKYWTEAEKTLADF
                     NAKAGKRRGGKWLFGGIAITIVATLITIFIVKSNKQQEETSQITKNTIQQQDLSKNID
                     DQTIQKEQNDKSNEVSKSVQQIKHKDAPVLRDEMRKKLREKRDHKLKEAKQEAKNNSD
                     NSRNKSVNQSNNPVNTQAGNIGNSSINPQNNANKQNVQPDNNDSHASTGLNQQDKSNT
                     EASGKAGGEEKTTVTDNSNQTTTNDQQEQHKTGEKGGNTEVNPDLNQPEQTTNGANEL
                     NNKVETPVNIQDSVNQNNISNGQNNPNKPSQFKTRKSTLGLQWHIGLQGGVYHTSPQF
                     KDVWNYSNVHVYPDPNTLFPAGEVSFGMMLPKVPFTLSSGIGLITMQDKSTYQINILN
                     IDTTYDYITDTSGLVTDSIPLLDSSYASYYNETPNKLTYVQIPLLIGYEWTRQSWHIG
                     IATGPVIGLKLGDSGIYETLVPTTYYDRVTWYWKAEPQVSYSFNEHWRLALQGNLQWQ
                     LSNTYLDSQIDLKFGLQGLQIGVQYDF"
     gene            complement(3009..3623)
                     /locus_tag="PLL28_07900"
     CDS             complement(3009..3623)
                     /locus_tag="PLL28_07900"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNF69286.1"
                     /translation="MLQHSLHTVPDFISTKGTRKTMVIEPSLLVLSAKGDKKAQHALY
                     KQAFSLLMGVCSRYATDRDEARSLLNQGFLKMLMHADQRKQNVPVEAWMRRVMINTII
                     DVYRQQKSYREHTKLVDYSDHSTVHAHEGSDDHRYMEKINADALFHMLTKLPEMTAKV
                     FNLFAIDGYSHKEIAEMLRMSEGTSKWHVNHARTLLKNMLNELD"
     gene            4060..6702
                     /locus_tag="PLL28_07905"
     CDS             4060..6702
                     /locus_tag="PLL28_07905"
                     /inference="COORDINATES: protein motif:HMM:NF009397.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HNF69287.1"
                     /translation="MAKKKQETPKEQAQPIQPIPVKQGTTHVSHMYKDWFLDYASYVI
                     LERAVPDIRDGYKPVQRRILHAMREMDDGRFHKVANVIGQTMQYHPHGDAAIGEALVN
                     MGQKDLLIETQGNWGDVRTGDDAAAPRYIEARLSKFALEVVFNKETTDWQLSYDGRKS
                     EPIYLPVKFPMVLAMGVEGIAVGLSTKILPHNFCELILASIEVLKGNETNILPDFATG
                     GMIDVSEYNHGERGGRIRCRAKIEVADKKTIVVKEIPFSTTTTSLIDSIIKANDKGKI
                     KIKKVTDNTAKDVEVAIELAPGISPDVTVDALYAFTDCEISISPSTCVIEDDKPLFLS
                     VDEILRRSTKNTRDLLKLELELKLQELNEKWHFSSLEKIFIENRIYRDIEECETWPDV
                     IKAIDKGLDPFKSKLMREVTEEDIVRLTEIKIKRISKFDAFKADEYIRDLEKEMKEVK
                     HNLRHLTDYAIKYFEELLRKYGKGRERKTEIRKFDTIEAKAVVANNAKLYVNREEGFV
                     GFGLKKDEFLCDCSDIDDIIAFTEDGKMKVVRIADKVFIGKKILYANVWKKEDERMTY
                     HMIYTDGKSGKTFAKRFNVTAITRDKEYDLTQGNAKSKVLYFSANPNSESEVVSIQLH
                     PSARAINKLFDFDFGTLAVKGRSSMGNTLSKYPMRKIIQKEKGTSTLGGRKLWVDETV
                     GRLNVDGRGRYLGEFDTGDHILVIYRDGNYELTNFELSNRFEMREILLLEKYNEQKVV
                     SAAYWDNEQKNMFVKRFYIESVVPDKKYLFISEAPGSKLYVASTAKKPRIEIIVVKGR
                     KQEEVPEIIEVESFIDVKGWKSLGKRLSSYEIRKVTGLPSDEPVEETPPPAKEGSVKV
                     GSQLEWDLDNDDKSGKDGKQTKLF"
     gene            6840..7421
                     /locus_tag="PLL28_07910"
     CDS             6840..7421
                     /locus_tag="PLL28_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69288.1"
                     /translation="MPAVKNDIFSKFMVPFMQWYLTMIAVTVVIDYILHRVGWVHMGI
                     YLGYLGTALVISSFIYSLRKRKIIQSGTPKKLLQWHEYGAWIGTIFILVHAGIHFNAI
                     LPWLAVLMMLLTVASGLVGKYILKKATESFRIKKQELLDAGKTPAEIDQVLLFDAVTV
                     EAMKKWRVVHMPITYFLAIFSIIHIITAIMYGA"
     gene            7418..8305
                     /locus_tag="PLL28_07915"
     CDS             7418..8305
                     /locus_tag="PLL28_07915"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNF69289.1"
                     /translation="MNKIVILMTALFVVVLVIKFPHRMINPGPLQEAHAALDNDCKNC
                     HQPFWGIPNANCISCHNPADIGRDTTGVDTGKILFHEALTNRSCIGCHDDHKGRIPLV
                     AINSFDHKMPGTAKGLNCVQCHTAPEDKLHPIVSKNCASCHDTKNWSSISAFDHAMIN
                     KVELQQCGTCHQAPQDQMHIAVTSTSCSNCHQTGAWSPATFNHDMYFVLDGNHNVRCN
                     TCHTKQDFKTYTCYGCHEHTERKMVAEHSEEGINNINDCARCHGSANEHDVKRSVGDG
                     NSGEHRKRKEGEHEEDDDD"
     gene            8352..8654
                     /locus_tag="PLL28_07920"
     CDS             8352..8654
                     /locus_tag="PLL28_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69290.1"
                     /translation="MKLFIATMLVIGTITGEKKSASFVLRNESASSIPLVIPGVMNPN
                     LSPMSNSKVTLEEGQEIFFLEKKKRYLLLEVSPELNGDTLIVNDLIKKRKQELDLN"
     gene            8658..9350
                     /locus_tag="PLL28_07925"
     CDS             8658..9350
                     /locus_tag="PLL28_07925"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNF69291.1"
                     /translation="MAHYLIIGASSGIGREVVNQLISSGHQVTATYNNHNADLDSAVT
                     WHHFNAIDAEPIINWMPDHLDGVVYCPGSIQLKPFHRIHAQQFADDYQLQVSGMIRIL
                     QAAYPALKKSSHAAVVMYSTVAVQRGFTFHTLVSASKGAIEGFARALAAEWAPSIRVN
                     VIAPSITDTPLAEGLLNTAEKIDANAQRHPLKRIGTAQDIAKMTTFLLSPDSAWITGE
                     ILHVDGGMHSIK"
     gene            9356..9817
                     /locus_tag="PLL28_07930"
     CDS             9356..9817
                     /locus_tag="PLL28_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNF69292.1"
                     /translation="MGVYTIYEEQFLPTSLEDAWKFVSDPHNLKVITPDYMRFNVLTK
                     DLPDSVYSGLIIQYYVSPVFRIPMKWVTEITFVEPLKYFVDEQRVGPYRIWHHEHHLK
                     AVDGGVLMIDRVTYQPPLGFLGSIANALFIAKQLKGIFVYRRKKLEQLFPQ"
     gene            complement(9820..12771)
                     /locus_tag="PLL28_07935"
     CDS             complement(9820..12771)
                     /locus_tag="PLL28_07935"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNF69293.1"
                     /translation="MQKVKYPISTLTLHTLALLILLVPQCTDMMAQVVEPGSKYASPV
                     HFIPNNGQLTDFNGDFVPNVYAYLQSGDVQVFVTSKGLTYMQYGEVKPDLSEGKKKVR
                     DWERIDMQLDGAKINAGNILFDEIGSEKMQYYVSGYDDPIVVQGCNRILIQEIYPGID
                     WELLVNEAGFKYNFIVRAHADLSQIRLHYITPLPISSPDGDIRIHAKQGDIIERAPYS
                     FLHATNEAVSSIILSEGKSEMGEIFRFQTGDYDHNEELIIDPSLWWCTYYGGVFNEIF
                     NCVDADLSGNIIAVGWTESANFPTYNAGTYYQGTKSVNEDVVIVKFSDIGERLWATFY
                     GGNAEDFGYGVSTDASGNIFVTGETVSSNFPVYNAGTYFQAGLNGDEDVFIIKFNAAG
                     TRQWATYYGGDNKEGGYSIATDNTGNIFVTGYTKSSTFPKLNAGTYYQASMAGVQDAF
                     MLKFSNTGTRLWATFYGGNAFDEGNALATDSDGDVFMTGTTKSTTLPVYDAGTYFQGT
                     MAGDTDAYVIKFSNTGSRLWASYFGGTDAEYGMSVVCDQNDNVIFGGYTQSSVLLTHV
                     PDLSWYWQIMNAGGTDMYIFETDNAGNSIWTTFFGGSGNEKMDTKDALDISGCNDLYI
                     SGTSFSYDLPLYDAGCSSYYDGTNAGEGDIWLTRFTDAGQITWSTLFGFENEDIASCL
                     ATSPLDGSSLFMTGVYHEYDPGEAVPLVNTGGTTYYDGSHNGDDECFVSKFIAVPLNL
                     DLSYSNLCYCLDSAIVTPSCGVAPYTYLWADGQTDSIATGLCPGLWDVEVTDADCNVE
                     TVTFLIDCILPLELINFTGYQDKGRNQLQWEITNDPVSEFFNLYRHFNGGDNLITSIP
                     VTNITDYHFSDDTFSEGIMYYTLEGISSTGEVIFSKTIAVEETSEKADLIITYPEYER
                     AKLILDAVDNESTYVDIVDMRGVVMMRFPWKLSPGRNYTYFEHPDLPKGYYLVTVSGG
                     TTDQYVGFYHQ"
     gene            complement(12921..13499)
                     /locus_tag="PLL28_07940"
     CDS             complement(12921..13499)
                     /locus_tag="PLL28_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNF69294.1"
                     /translation="MNPNVDFYFDKAGKWENEIRALRKTILSCQLQEELKWGTPCYTL
                     DGRNIVLIHIFKEYCAVLFFKGVALQDAQKLLIQQTENVQTARQMRFTSLSEIKKTTT
                     ALKAYIFEAIEVEKSGVKMPLKKTKEFSMAEEFKSALNKSTKLKKAFEALTPGRQRAY
                     LLHFSSAKQSATRIARIEKYVPKILAGKGIDD"
     gene            complement(13503..13889)
                     /locus_tag="PLL28_07945"
     CDS             complement(13503..13889)
                     /locus_tag="PLL28_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNF69295.1"
                     /translation="MNKRNKIIYWVATLWLSLGMTSTAIVQLSHKQSEVDFIHELGYP
                     TYLLTIIGIWKIAGVVIVLKPKFTLVKEWAYAGFFFCMSGAIFSHIAVSHTFTEMAPA
                     MLLLVLTIISWYMRPVDRKFTSLQKA"
     gene            complement(13891..14361)
                     /locus_tag="PLL28_07950"
     CDS             complement(13891..14361)
                     /locus_tag="PLL28_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNF69296.1"
                     /translation="MTDQKTIVTAPEHRQEIVITRTFDLPVDLLFMAYEDAEIVAQWM
                     NTKVLKLESKQHGSYRFETTDPMGNVHGFNGTIHSFEKNASIVRTFEMENGEFPVQLE
                     YLTFEKLGDDKSKLTMHIVFQSIEARDRLLKLPFAMGINMAHNRIQEIQSTSKQ"
     gene            complement(14358..14687)
                     /locus_tag="PLL28_07955"
     CDS             complement(14358..14687)
                     /locus_tag="PLL28_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNF69297.1"
                     /translation="MNLRRDVFQALADPTRRTILLLLTTQAMTAGAIASNFETARPTV
                     SKHLHILTECELLQAEQNGREILYHLNLDKMKEIADYLEPFRRMWDERFNKLENIMQH
                     YHHKPKP"
     gene            complement(14783..>14879)
                     /locus_tag="PLL28_07960"
     CDS             complement(14783..>14879)
                     /locus_tag="PLL28_07960"
                     /inference="COORDINATES: protein motif:HMM:NF025432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HNF69298.1"
                     /translation="FGDFRYATVFVYHNGAESYYAARGFRGMLLV"
BASE COUNT         4361 a         2984 c         3074 g         4460 t
ORIGIN      
        1 tatgtgacct ttataccgga taacaccatc ttccgcaatt ttcattttat tcccgaatac
       61 ctggataatc aattgtggaa ttatgtattc ctgaattccg gactcaccat caatttcaat
      121 ggtcagaaat actattcaca gaatggtttg catgatctgt tagtgcgcaa tagcaatgca
      181 gaaacaaatc gctaccccat tatacatctt aagggcgatg atattgaaat tgcacttaca
      241 cacggcaatg aatatggaga agattattat tcattcgtaa acggacaaaa taccacacag
      301 ggtggcacgc atttggcggc gtttcgcgaa ggtttggtga agaccgtgcg cgaattctat
      361 aagaaagatt ttgatgcatc cgacatccgt caggctattg tgggcgctat tgccatcagg
      421 gtgcaagagc cggtgtttga atcacagaca aagaccaaac tcggttcgat atatacctct
      481 cctgatgcac aggtaactgt gaaatcattt gttgtggatt ttgtcaagaa agagcttgat
      541 aattatctcc ataaaaatcc tgctactgcc gatgccttgt taaaacgcat tcttcaaagc
      601 gaacgtgaac gcaaagatat tgcaggtatc aagaaactag caaatgagcg cgcgaagaag
      661 gcaaacttgc ataacaggaa attgagagat tgccgatatc atcttggaga agataaacat
      721 gagaaaacgc tacgcagtac tttatttatc actgagggtg atagtgccag tggaagtatc
      781 accaaagcgc gcgatgttga atcgcaagca gtattcagct tgaaaggtaa gccactgaat
      841 gttttcggac tgacgaaaaa aattgtttat gaaaatgagg agtttaatct ccttcagcat
      901 gcattaaata ttgaggatgg gattgacggt ttgcgctata atcaaatcgt agttgcaacc
      961 gatgccgatg tggatggcat gcatattcga ttgctattac tgactttctt tttgcagttt
     1021 ttccctgaag tagtgaaaaa tggtcatctc tatattttgc aaacgccatt attccgtgtg
     1081 cgaaataaaa aagagacaat ttattgctat aatgaagaag agaaacaggt tgcagtcaat
     1141 aaattgggaa ataaacctga gatcacacga ttcaaaggac ttggagaaat atctccggat
     1201 gaattttcag gcttcattgg tgaaaatatt cgccttgaac cggtaattat cggtaaagaa
     1261 caatcgatcc agtctacact tgaatattac atgggaaaaa acacccctga tcgccaaaca
     1321 tttatcattc gcaatttacg cgttgagaag gatgtgatcg atgagcctgc gatcgttgag
     1381 gctgaatcag aagtttaaaa atcgtactgc acaccaattt gtaatccttg cagaccgaat
     1441 ttgagatcaa tttgggaatc caaataagta ttggaaagtt gccattgtaa atttccttgc
     1501 aatgccaaac gccaatgttc attaaaactg tatgacactt gcggctcggc cttccaatac
     1561 caggtaacac gatcataata tgttgttggt accaatgttt catatattcc actatctccc
     1621 agttttaatc ctattacagg acccgtggca atgccaatat gccaactttg cctcgtccat
     1681 tcatatccaa taagcagcgg aatctgcaca taggttaact tatttggcgt ttcattataa
     1741 taagatgcat aactgctatc taacaacgga atgctatcgg tcaccaaccc ggaagtatct
     1801 gtaatataat cgtatgtggt gtctatgttt agaatattaa tttgatatgt tgatttatct
     1861 tgcattgtaa ttaaaccaat acctgagctc aaggtaaatg gcaccttagg caacatcata
     1921 ccaaatgata cttcaccggc agggaatagg gtgtttggat cagggtagac atgcacattg
     1981 ctgtaattcc aaacatcttt aaattgaggg cttgtatggt aaactccgcc ctgcaatcca
     2041 atatgccatt gtaatccaag ggttgatttt cttgttttga attgagaagg cttattagga
     2101 ttattctgac cattagaaat attgttttga tttacggaat cctgaatgtt aacaggcgtt
     2161 tcgactttat tattcaattc atttgcgcca tttgtagttt gttccggttg attcaaatca
     2221 gggttaactt cagtattacc acctttttca cctgttttgt gctgctcttg ttggtcgttg
     2281 gttgttgttt gattcgaatt gtctgtgaca gtagtttttt cttcgccacc tgccttgcct
     2341 gatgcttcgg tgttgctttt atcttgctga ttcaatccgg ttgatgcatg tgagtcattg
     2401 ttgtcaggtt gcacattttg tttatttgca ttgttctgtg gattgatact gctattaccg
     2461 atattcccgg cttgggtatt aacgggattg tttgattgat tgacggattt atttcttgaa
     2521 ttatcgctat tatttttcgc ttcttgcttc gcttctttta atttatggtc gcgtttttca
     2581 cgaagttttt tccgcatctc atctcgtaat accggtgcat ccttgtgctt gatttgttga
     2641 acacttttgc ttacttcgtt gcttttgtcg ttttgttctt tttggatagt ctgatcgtca
     2701 atatttttag ataggtcttg ctgttggatt gtatttttcg tgatttgtga agtttcttcc
     2761 tgttgcttgt ttgatttcac aatgaaaatc gtaatcaagg ttgccacaat tgttatggca
     2821 atgccaccaa ataaccattt cccgccgcga cgctttcctg ctttggcatt gaaatcagca
     2881 agtgttttct ctgcctctgt ccaatatttt tcatcatacg ggaagtcgcg attctccagg
     2941 ctgtcgcgaa acagcttgtc gatcgggtga tcttccggtt tttgtggttt tttgtgtgcc
     3001 ataatgcttt aatcgagttc gttaagcata ttttttaata atgtacgtgc atggttgaca
     3061 tgccatttcg atgtgccttc gctcatcctt agcatctctg caatttcttt gtgactatat
     3121 ccgtcaatcg caaacaggtt gaatactttc gcagtcattt caggcagttt tgtaagcata
     3181 tggaataatg catcagcatt gattttttcc atataccgat ggtcatcact tccttcatgt
     3241 gcatgaaccg ttgaatggtc ggagtaatca accagtttcg tgtgttcacg atatgacttt
     3301 tgctgcctgt agacatcgat aattgtatta atcatgactc tccgcatcca ggcttcaacc
     3361 ggaacatttt gctttcgctg atctgcatgc ataagcatct ttagaaaacc ttggttaagt
     3421 aagctcctcg cctcatcacg atcggttgca tacctgctgc aaacacccat aagcaagctg
     3481 aatgcctgct tatataaagc gtgctgggct tttttatcgc cctttgcaga taataccaat
     3541 agcgatggtt ctatgaccat tgttttcctt gttccttttg tgctaataaa atcaggtaca
     3601 gtatgtaaac tgtgttgaag cataagcgat tacatcatac tatcaacctt tcatgctccc
     3661 atgcaaaatt agaaaaacca ccatcccgaa cgcatccggg cggtggtatt ttgactagca
     3721 ctaaggaaca cagtgaacga aaactgctct tgcccaagca taacaaagcc acatctgaca
     3781 gaactttcag ggtattggaa gatgtagttt gcctcatgtt cgggtacgga ttttaccccg
     3841 ttatggctta tcacgcattc ggctttgaaa aggttgggtt gggcgtaagc ttttttttac
     3901 gacttttcgc aacacgaaaa gcaacatttg cgatttttgc aactccccga agtcatttgc
     3961 acaataattt tgtatttgtc ctaatgccca tcaaatcgtg gaaaactcgc ttcataagcc
     4021 ataggcattg cagtattttt gaccctcacc gcggcagtta tggctaagaa gaagcaagag
     4081 acacctaagg aacaggctca acctattcaa ccgattccgg taaaacaggg gactacccat
     4141 gtttcacata tgtataagga ttggtttctc gactatgcca gctacgtgat cctggagcgc
     4201 gcagtacctg atatccgaga tggatataaa ccggtgcaac gtcgcatttt acatgccatg
     4261 cgtgaaatgg acgacggtcg tttccataaa gttgccaatg taattggtca aaccatgcaa
     4321 tatcaccctc atggtgatgc tgctatcggc gaagccttgg tgaacatggg acaaaaagac
     4381 ctcctcattg aaacgcaagg aaactggggc gatgtgagaa ccggcgacga tgccgccgct
     4441 ccacgatata ttgaagcacg actttcaaag ttcgctctgg aggtggtatt taataaagaa
     4501 actaccgatt ggcagctgag ttacgatggt cgtaaatctg aacctattta tctgccggtt
     4561 aagtttccta tggtacttgc catgggcgtt gaaggtatcg ccgttggctt gtcgacaaaa
     4621 attttaccgc ataatttctg tgagttaatt ctagccagca tagaagtgtt aaaaggaaac
     4681 gagactaata ttcttcctga ttttgcaaca ggaggaatga ttgatgtttc ggaatacaac
     4741 catggcgagc gtggtggaag gattcgatgc agggcgaaaa ttgaagttgc tgataaaaag
     4801 acaattgttg tgaaagaaat tcctttcagc acaacaacta cttcgttgat tgattccatc
     4861 atcaaagcca atgataaagg caagataaaa atcaaaaagg ttaccgataa tactgcaaaa
     4921 gatgtagaag tagcaatcga acttgcaccg ggaatttcac ctgatgtgac ggtggatgct
     4981 ttatatgctt ttaccgattg cgagatttca atttcaccat caacttgtgt gatcgaagat
     5041 gataagcctt tgtttttgtc ggttgatgaa atactgcgca gaagtaccaa aaatacccgc
     5101 gacctgctga aactggagct ggaattaaag ctgcaggaac tcaatgagaa gtggcatttc
     5161 agctcgcttg agaaaatatt catagaaaat cgcatctatc gcgacattga agaatgtgaa
     5221 acctggcctg atgtaattaa agctatagat aaaggcctcg atccattcaa atctaaactg
     5281 atgcgcgagg ttacggaaga ggatattgtt cgccttacag agatcaaaat caaacgcatc
     5341 agtaagttcg atgcatttaa ggctgatgaa tacatccgcg atcttgaaaa agaaatgaag
     5401 gaggtgaagc ataatttacg tcacctgaca gattatgcca tcaaatattt tgaagaactg
     5461 cttcgtaaat atggcaaagg ccgtgaacgg aaaacagaaa ttcgcaaatt cgataccatt
     5521 gaggcaaaag cagttgttgc aaataatgca aaattgtatg taaacagaga ggaaggtttc
     5581 gttggattcg gattgaagaa agacgaattc ctttgcgatt gctccgatat tgacgatatc
     5641 attgcattta ccgaagatgg aaagatgaag gttgtgcgta ttgccgacaa agtattcatt
     5701 ggtaaaaaaa tcctctatgc caatgtttgg aaaaaggaag acgaacgcat gacctatcat
     5761 atgatttata cggatgggaa gtcgggcaaa acttttgcca agcggtttaa tgtaacggct
     5821 attacgaggg ataaagaata tgatctcaca caaggtaatg caaaaagcaa agtgctttat
     5881 ttttcagcaa atccaaatag cgaaagtgag gtggtgagca tacaactgca tcctagtgca
     5941 cgtgctatca ataaattatt cgacttcgat ttcggaactc tcgctgttaa aggaaggagc
     6001 agtatgggaa atacgctttc gaagtatccg atgcgtaaga taatacagaa agaaaaagga
     6061 acatcaacac ttggtggaag aaagctctgg gtggacgaaa ctgttggcag attaaatgtc
     6121 gatggtcgtg gtcgatacct cggcgaattc gatacgggcg atcacatttt agtgatttat
     6181 cgcgatggta attacgaact caccaatttt gagctcagca atcgcttcga aatgcgtgag
     6241 atattattgc tagagaaata taatgaacaa aaagtagtat ccgcagcata ttgggataat
     6301 gagcaaaaga atatgttcgt gaagcgattc tatatagaat ctgtcgttcc cgataaaaaa
     6361 tacctcttca tctccgaagc tcccggttca aaactgtatg ttgcctctac tgctaagaaa
     6421 ccacgtatag aaattattgt tgtaaaaggc cgtaagcaag aagaagttcc tgagatcatt
     6481 gaagtggaaa gcttcatcga tgttaaggga tggaaaagtt taggcaagcg attgagttcc
     6541 tacgagatac gaaaagttac aggtcttcca tcagatgaac ctgttgagga aactccacca
     6601 cctgctaagg aaggctctgt aaaagtgggt agccaactcg agtgggatct cgacaacgat
     6661 gacaaatccg gtaaagacgg caaacaaacg aaattgtttt gagccggcaa acgtagaatt
     6721 tgtctaagta atttattttt caaaataagt gtaaactgac gaatatcacc gctcggtagg
     6781 atatcatgat gcaattttgg aattgccatt atcgatgaat tgcgataatg tgcaatttca
     6841 tgccggcagt taaaaatgac attttttcga agtttatggt gccatttatg cagtggtacc
     6901 tcacgatgat tgccgttaca gtggtgattg attatattct gcatcgtgtt ggttgggtgc
     6961 acatgggtat ttatctcgga tacctcggaa cggcactagt aattagttcg tttatttata
     7021 gtcttcgcaa gcggaaaatt attcaatccg gaacacccaa aaagttgctg caatggcatg
     7081 aatatggtgc atggattgga accatattca ttctggttca tgcaggtata cattttaatg
     7141 cgatacttcc atggctggct gttttgatga tgctactcac tgtggcaagc ggtttggtag
     7201 gtaaatatat tctcaaaaaa gcaaccgagt cattcagaat taaaaaacag gaattgctcg
     7261 atgccggaaa aaccccggct gagatcgatc aggtattatt gtttgatgca gtaactgttg
     7321 aagcaatgaa aaagtggcgt gtggtacata tgcctatcac ctattttttg gccatatttt
     7381 ctatcatcca tatcatcact gcaataatgt acggcgcatg aacaagatcg tgattttgat
     7441 gactgccctt ttcgtggttg tactggtaat aaaattccct catcgcatga tcaaccccgg
     7501 tccgcttcaa gaggcacatg cagcattaga taacgattgc aaaaactgtc accaaccatt
     7561 ttgggggatt ccaaatgcaa attgtataag ctgtcataat cctgcagata tcggtcgtga
     7621 tactacaggt gttgataccg ggaaaatatt atttcacgaa gcgttgacaa atagatcatg
     7681 tatagggtgt catgatgatc acaagggaag gattccgctg gtcgctatca attcattcga
     7741 tcataagatg ccgggaactg cgaaaggttt gaattgtgtc cagtgtcata cagctcctga
     7801 agataagctt catcctattg tttctaaaaa ttgcgctagt tgtcatgata caaaaaattg
     7861 gtcatcaata agcgcatttg accatgcaat gattaataag gtagagctgc agcagtgtgg
     7921 tacatgccat caggcgcccc aagaccaaat gcatatcgca gtcacttcaa catcatgttc
     7981 aaattgccat caaaccggag catggtcacc ggcaacattt aaccatgata tgtattttgt
     8041 gcttgatggt aatcataatg tacgatgcaa cacctgtcac acaaagcaag atttcaagac
     8101 gtatacctgt tatggatgtc atgagcatac ggaaagaaaa atggtggctg agcattccga
     8161 ggaaggcata aataatatca atgattgcgc acgatgccat ggcagtgcca acgagcatga
     8221 tgtaaaacga agtgtcggag atggcaattc gggcgaacat cgcaaacgga aggagggaga
     8281 acatgaggaa gatgatgatg attgaactcg ctgagctttg aacaatttca ttgtacatcc
     8341 gtttgccatg catgaaacta ttcatcgcta caatgctagt gatcggtacg atcacaggag
     8401 aaaagaaaag tgcaagcttt gtgctgcgca atgaatctgc ctcctccatt ccactcgtta
     8461 tacccggagt gatgaatcca aatctatccc caatgtcgaa cagcaaagtt accttggagg
     8521 aagggcaaga aatatttttt ctggagaaga aaaaacgtta cctactcttg gaagtaagcc
     8581 cggagctaaa tggtgatacc cttattgtca atgatttgat taaaaagcgc aagcaggaat
     8641 tggatttaaa ttaaattatg gcacactatc tcatcattgg cgcatcatca ggcattggca
     8701 gggaagtggt aaatcaatta atcagttccg gacatcaggt aacagcaacc tataacaacc
     8761 ataatgctga tttagatagt gcggtaacct ggcatcattt taatgccatc gatgctgaac
     8821 ctatcatcaa ttggatgcca gatcatcttg atggcgtggt ttattgcccc gggagcatac
     8881 aactcaaacc atttcatcgc attcatgcgc agcaatttgc cgacgattat caattgcagg
     8941 taagtggtat gattcgcata ttacaggctg catatcctgc attgaaaaag tcatcacatg
     9001 cggcagtagt aatgtattca accgttgcag ttcagcgtgg attcactttt cataccttgg
     9061 tatctgcatc taaaggagcc attgaagggt ttgccagagc gcttgctgcc gaatgggctc
     9121 cctcaattag agtaaatgtt attgcgcctt ctatcacaga tacaccgctg gcagaaggat
     9181 tactcaatac ggctgaaaaa attgatgcga atgcgcaacg tcatcccctc aaacgcattg
     9241 gcactgctca ggatatcgca aaaatgacta cttttctgct ctcgccggat agcgcatgga
     9301 taaccggaga aatattgcat gttgacggag gcatgcatag cattaaataa ttattatggg
     9361 agtttataca atatacgaag aacaattttt gcctactagt ctcgaagatg cctggaagtt
     9421 tgtttcagat ccgcataatc ttaaagtgat tactcctgat tacatgcgtt ttaatgtgct
     9481 taccaaggac cttccggata gtgtttattc cggattgatc attcaatatt atgtttcacc
     9541 ggtatttcgg ataccaatga aatgggttac cgaaataacg tttgtcgagc cattgaaata
     9601 ttttgtagac gaacagcgcg ttggcccata ccggatctgg catcacgaac atcatttaaa
     9661 agctgttgac ggtggtgtgt tgatgataga tcgcgtaacc tatcaaccac cacttggttt
     9721 tctcggttct attgcaaatg cgctgtttat tgctaaacaa ttgaaaggta ttttcgtcta
     9781 ccgcaggaaa aaattagagc agttgttccc gcaataactt tattgatgat agaaccctac
     9841 atactgatcg gtagttccac cgcttacagt cactaagtaa tatcctttcg gcaaatcagg
     9901 atgttcaaag taggtgtaat ttctgcccgg acttaatttc cacggaaaac gcatcataac
     9961 aacgccccgc atgtcaacaa tatctacata agtagattca ttgtctactg catcgagaat
    10021 tagttttgcg cgttcgtatt cagggtaagt gataatcaaa tctgctttct ctgaggtttc
    10081 ttctacagca atagttttgg agaatattac ttcgccggtg gaggatatgc cttccagtgt
    10141 gtaatacata ataccttcgc tgaaggtatc gtcgctgaaa tggtaatctg taatattagt
    10201 gacaggaatg cttgttatta agttatcacc gccattaaaa tgacgataca gattgaaaaa
    10261 ttcactgaca ggatcatttg taatttccca ttgaagctga tttctgcctt tatcctgata
    10321 acctgtgaaa tttattaatt caagaggtag tatacagtca attaaaaacg ttactgtttc
    10381 cacattacaa tcggcatcag taacttctac atcccataat cccggacata aaccggtagc
    10441 tatggaatct gtttgcccgt cagcccaaag gtaggtgtat ggagccacac cgcaagaagg
    10501 tgtaacaatt gcggaatcga ggcaatagca taagtttgaa taggagagat caagattaag
    10561 tggaacagca ataaattttg atacaaaaca ttcatcgtcg ccattatgtg atccatcgta
    10621 ataggttgtg ccgcccgtat tgacaagagg aactgcttca cccggatcat attcatgata
    10681 tactccggtc ataaacaatg agctgccatc aagtggtgat gtagcaagac atgaagcaat
    10741 atcttcgttt tcaaatccaa aaagtgtcga ccaggtgatt tgcccggcat cagtaaatct
    10801 ggtaagccag atatctccct ctcctgcatt ggtgccatcg taataactgc tgcacccggc
    10861 atcatataat ggcaggtcgt atgaaaagct ggttcctgaa atatacaggt cattacatcc
    10921 actgatatct aatgcatctt tagtatccat tttctcatta cctgagccac caaaaaatgt
    10981 tgtccagata ctattgccgg cattatctgt ttcaaaaata tacatatctg ttccgcccgc
    11041 attcatgatt tgccaatacc atgagagatc aggaacatgt gtaagcaaaa cagaagattg
    11101 ggtatatccg ccaaaaatca cattatcgtt ttgatcacaa actacactca ttccatattc
    11161 tgcatcagta cctccgaaat aactcgccca taatcgactt ccggtattac tgaattttat
    11221 tacataagca tcggtatctc cggccatcgt tccttgaaaa tatgttcctg catcataaac
    11281 cggcagggta gtggatttcg ttgttcctgt cataaataca tcgccatcgc tgtcggtagc
    11341 caatgcattc ccttcgtcaa atgcgtttcc gccgtaaaat gttgcccata atctggtgcc
    11401 tgtgttagag aatttcaaca tgaaggcatc ctgcacacct gccatactcg cctggtaata
    11461 ggtgccggca tttaattttg gaaaggttga cgatttggta tatcctgtaa cgaaaatatt
    11521 tccggtattg tctgttgcaa tgctatagcc accttcttta ttatcgccac cataataggt
    11581 tgcccactgt ctggttcctg cagcattaaa tttgatgata aagacatcct catcaccatt
    11641 taatccggct tgaaaatagg tgcctgcgtt gtatacaggg aaattacttg aaaccgtttc
    11701 gcccgttaca aaaatatttc cggatgcatc agtggacacg ccataaccga aatcttctgc
    11761 gttgccacca taaaacgttg cccataagcg ctctccgata tcagaaaatt ttacaatcac
    11821 tacgtcttca ttaactgatt ttgtaccctg gtaataagta cctgcattat atgtcggaaa
    11881 gtttgcagat tctgtccatc ctacggcaat gatatttcct gaaagatcag catcaacaca
    11941 attaaaaatt tcattaaaaa caccgccata ataagtgcac caccataaag aagggtcaat
    12001 aattaattcc tcattgtgat cataatctcc ggtttgaaaa cggaatattt cacccatctc
    12061 agattttcct tcacttaaaa ttatgcttga tactgcttcg tttgtcgcat gcagaaaaga
    12121 gtagggggct cgctctatga tatcaccctg tttggcgtga atgcgaatat caccatcggg
    12181 tgaagaaatg ggtaggggcg taatgtaatg caagcgtatc tgtgataagt ctgcatgagc
    12241 acgcacaata aagttatatt taaacccggc ctcgtttacc agcaattccc aatctatgcc
    12301 gggataaatt tcctgaatta atattctatt acatccttga actacaatcg gatcatcgta
    12361 tcctgaaacg taatactgca tcttttcact tccgatttca tcgaagagaa tattgccggc
    12421 atttattttg gcaccatcca attgcatatc aatccgctcc caatcgcgaa cttttttctt
    12481 tccttctgaa agatcaggct ttacctcacc atactgcatg taggtaagcc ctttcgatgt
    12541 gacaaatacc tgcacatcgc cggattgtaa ataagcgtaa acattaggca caaaatcgcc
    12601 attaaaatca gtaagctgac cattgtttgg aatgaaatga acaggtgaag catatttgct
    12661 cccgggttca acaacttgtg ccatcatgtc ggtgcactgc ggtacaagca gaattaacaa
    12721 tgccagtgtg tgcagagtta gggtcgaaat tgggtatttc actttctgca tgatacaaag
    12781 gtgatgcata cccatgaccc gtaataggac aaatatcacc aacttccacg aaaaccggat
    12841 tttctcaaaa gttgaatttt cagctgaaac actgactggt atttattttc agacttaatt
    12901 agaattaatc caaagaatta ttaatcatca atgcccttgc ccgctaatat tttcggaaca
    12961 tatttttcaa ttcttgcaat gcgtgttgcc gattgttttg ctgatgaaaa gtgcagtaaa
    13021 tatgcgcgct gccgtccggg tgttaatgcc tcaaatgctt ttttgagttt tgtactttta
    13081 ttgagtgccg atttaaactc ttctgccatt gaaaattctt ttgtcttttt taaaggcatt
    13141 ttaacgccgg atttctcaac ttcaattgct tcgaaaatat aggctttgag tgctgtggtt
    13201 gttttcttta tttcagaaag cgaagtaaag cgcatttgtc ttgccgtctg cacattttca
    13261 gtttgctgaa tcaggagttt ttgagcgtct tgtaatgcaa ctcctttgaa gaataagact
    13321 gcgcagtatt ctttgaaaat atgtatcaaa acaatattgc gaccatcaag cgtataacat
    13381 ggcgttcccc atttcaactc ttcctgcaat tggcagctta aaattgtctt ccgtaatgca
    13441 cggatctcat tttcccattt gccggcttta tcgaaataaa aatctacatt cggattcatt
    13501 gatcaggctt tttgaagtga agtgaatttt ctgtcaacag gtcgcatgta ccatgaaata
    13561 atggttaaaa caagcaataa catcgccggc gccatctctg taaaggtatg cgatacggct
    13621 atatgcgaga aaatggctcc cgacatgcag aagaaaaatc ctgcataagc ccattctttc
    13681 actaaagtaa atttgggctt caaaactatt acgaccccgg ctattttcca tatgccgata
    13741 attgtcaata agtaagtagg ataaccaagt tcatgaatga aatctacttc tgattgcttg
    13801 tgactcagtt gcacgatagc tgttgatgtc atccccagcg acagccataa agtagctacc
    13861 caataaataa ttttattacg cttgttcata ttattgtttt gaagttgact gaatttcctg
    13921 aatgcggtta tgcgccatat tgatacccat ggcaaatggt aatttcaata agcgatctct
    13981 ggcttcaatc gattgaaaaa caatgtgcat ggttaattta cttttatcat ctcccaattt
    14041 ttcgaatgtg agatattcca attgcaccgg gaattcgcca ttttccattt caaatgtgcg
    14101 cactatggaa gcattttttt cgaaactgtg aatggttccg ttaaacccat ggacatttcc
    14161 cattggatct gttgtttcaa atcgataact gccatgttgt tttgattcca gttttagcac
    14221 cttggtattc atccactgcg ctacaatttc ggcatcctca taagccatga ataataagtc
    14281 gacaggtaaa tcaaatgttc tggtgatcac tatctcttgt ctatgctcag gtgctgtaac
    14341 tattgttttc tgatctgtca tggttttggt ttatgatgat aatgttgcat gatgttttcc
    14401 aatttattga agcgttcatc ccacattctg cggaatggct ccagataatc agctatttct
    14461 ttcattttgt caaggttgag gtgatacagt atttccctgc cattctgttc tgcttgcaaa
    14521 agctcgcatt cagtgagaat gtgcaaatgc tttgaaacag ttggcctggc agtctcgaaa
    14581 ttcgaggcga tggcaccggc agtcatggct tgtgtagtca gcaataacaa aatggtgcgt
    14641 cgggttggat cggctaaggc ttgaaataca tctctcctga gattcattat gtagctattt
    14701 ggctacaaat atatatgtag ctaaatgact acgcaaaata attggtcaac tttaacattt
    14761 gagtcgaact gaaacggcgg tttcaaacga gcagcatgcc tctaaaaccc ctcgcagcgt
    14821 aataagattc tgcgccgttg tggtaaacaa aaacagtagc atacctgaaa tcaccaaaa
//
LOCUS       DAOQHY010000079        14644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_1969_length_14644_cov_5.311399, whole genome shotgun sequence.
ACCESSION   DAOQHY010000079 DAOQHY010000000
VERSION     DAOQHY010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14644
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1969_length_14644_cov_5.311399"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..978)
                     /locus_tag="PLL28_07965"
     CDS             complement(178..978)
                     /locus_tag="PLL28_07965"
                     /inference="COORDINATES: protein motif:HMM:NF018770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HNF69299.1"
                     /translation="MTPSTLYTCLVMHNRLSPKKHRFNYKVFMFFWDIDNSAEIAKSH
                     RLLSYNKGGVFSFRDRDHFKYPEGDNRNELTVRAKLNAWLKENGVVDMPEKVMLLTHI
                     RMFGYVFNPVSFYFCYNASGDCLYVVTEISNTFGEMKIFLIDQKQQQSFEQEDVKYFY
                     VSPFTDMDTSFRFKYQLPSDKLNLRIDVQDRSGDRFFISTLTGARRTLSDLRLLWYVV
                     RFPFITLQVIGAIHWHAFLLWLKKVPWHRKTEQASLQRDILNKSGQPD"
     gene            complement(1015..2274)
                     /locus_tag="PLL28_07970"
     CDS             complement(1015..2274)
                     /locus_tag="PLL28_07970"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNF69300.1"
                     /translation="MARIAIIGTGIAGMGAAWYLHRDHEITVFEADNRIGGHTNTVFV
                     EEPGQDIPIDTGFIVFNNHTYPRLTALFDALQVKVKKTDMSFAAQICDEHLEYCGSSL
                     NKLFAQRRRIFSPRYIRFLKQLNTFNKTCIEVFHNETLSALSIKDYLRMKKYSDDLLN
                     WYILPMSSALWSTPPDTTAQFPVLSLVRFFSNHGFLGLNTQFQWYTVSGGSEQYKQKL
                     IAPFKERIFVNQGIRSIRRTSDGVELTDVHGSVFQFDKVIVAAHGDQALKMWSDASQE
                     ERALLSPFSYQKNVATLHTDGSIMPKKKMVWSSWNYRTQTRGNNLVSSCTYWMNSLQD
                     VSDFTDYFVTINDPGIIDGNKVIKEIVYEHPVFTVEAMKAQAKLPELNRNGSLYFCGS
                     YFRYGFHEDALMSAANVCEQILGRKVI"
     gene            complement(2276..3310)
                     /locus_tag="PLL28_07975"
     CDS             complement(2276..3310)
                     /locus_tag="PLL28_07975"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HNF69301.1"
                     /translation="MINTLLERDRIPDFLIRARIRSLLRRRLKDESFSTAEQQQLHLM
                     ELIGQLKASPIAIETGAANEQHYEVPTAFFSMVMGKHMKYSCCYWDDNTPDLSSAEAK
                     ALEITCQRAQLREGMNILELGCGWGSLTMFMAQKFPSAHVTGVSNSSTQKLHIESQCK
                     MRGITNVRIITADMNVFDINETFDRVVSVEMFEHMRNYQLLLEKISRFLKPDGKLFVH
                     IFTHKEFTYFFDVVDDTDWMSKYFFTGGVMPSDDLLFYFNDHLTIQDHWHWDGTHYGK
                     TSEAWLSNMDAHKNEIMPILAETYGAEQSVKWWVYWRIFFMACAELWNFNNGREWIVS
                     HYLFTKKATV"
     gene            3600..4028
                     /locus_tag="PLL28_07980"
     CDS             3600..4028
                     /locus_tag="PLL28_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69302.1"
                     /translation="MVKQFGLPNYVIWLFGGIAFVAVGTISVFHWTYGLATFILTTGV
                     AFAGYWFFFRPAFVALDIARKGEPAMAIILQVWETGRTVNDNPRIKLLLEIRHSTMVP
                     YEVEVRQNIAKDDMRLYQPGRTLQVKVDSRNPRKVAILAA"
     gene            4078..4488
                     /locus_tag="PLL28_07985"
     CDS             4078..4488
                     /locus_tag="PLL28_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNF69303.1"
                     /translation="MVYLTRRETFNAAHRVYNNAWSEEKNKEMFGKCANKNWHGHNYQ
                     LFVTIKGKPDPDTGFIMDAKKLSDVIKREIVERFDHMNLNMDTPYFTDQQPSTENFCV
                     VIWALIEPFITGGVLHCIRLQETENIYAEYYGEQ"
     gene            4562..5131
                     /gene="folE"
                     /locus_tag="PLL28_07990"
     CDS             4562..5131
                     /gene="folE"
                     /locus_tag="PLL28_07990"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNF69304.1"
                     /translation="METLDNLIHHYQGIITSLGEDAEREGLVKTPERIAKAMQFLTQG
                     YNQDAEAVIRSAMFNEECSEMVIVKDIELYSMCEHHMLPFFGRAHVAYIPNGKIVGLS
                     KIARVVDIFSRRLQVQERLTHQILEALQNTLNPHGVAVVIEASHLCMMMRGVQKQNSV
                     TTTSAFIGQFQEVQTRNEFINLIAARLSK"
     gene            5194..5679
                     /locus_tag="PLL28_07995"
     CDS             5194..5679
                     /locus_tag="PLL28_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69305.1"
                     /translation="MAILHFVVTFTAKLNAMPNLRSTILMLFICSGLIAQAQPKQVKL
                     KGHKCPSIAISDPGEFYSTYNFSDNDLHALDSAGLKSTVIENIIAHHTETSWPGKLAS
                     LDERIANPDILKSYVVYKVAEINNMVVVLVPAKYNSDKPEGWALTEDVYFILSKAGVN
                     L"
     gene            5692..6567
                     /gene="fabD"
                     /locus_tag="PLL28_08000"
     CDS             5692..6567
                     /gene="fabD"
                     /locus_tag="PLL28_08000"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNF69306.1"
                     /translation="MKKAYVFPGQGSQFPGMGKDLYENNEAARALFERANEILGFRIT
                     DIMFNGTEEELKQTKVTQPAIFLHSVILASTLSDFNPDMVAGHSLGEFSALVANGALS
                     FEDALRLVSARAQAMQKACELQASTMAAVLGLADEIVEKICAETPGIVVAANYNCPGQ
                     LVISGEVEAVNKACEALKAAGAKRALLLPVGGAFHSPLMEPARIELSAAIEATPFNTP
                     RCPVYQNVTASAIIDPAEIKNNLLIQLTAPVKWTQSVTQMTADGATHYIEVGPGKVLQ
                     GLVKKISPTAEVASA"
     gene            complement(6643..7848)
                     /locus_tag="PLL28_08005"
     CDS             complement(6643..7848)
                     /locus_tag="PLL28_08005"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNF69307.1"
                     /translation="MAKKKQAKGFGSICVQPMATERKILSHNEPIFATTAYGFESLDH
                     ANMLFDDPSKGYVYSRWGNPTIEMAEKKIALLETSGSDIDATAQLFSSGMAAIAAAMT
                     SCATSGDTILTQHQLYGTTDELMQKQLAAWNINTMRMDLNDFDAVEKALKKNKKITLV
                     YIETPSNPMMSVTDIQTVAEIAHRYGRMVAVDNTFASPYCQKPLLLGADLVIHSATKY
                     LNGHGNGLSGVVVSRDRNFMRERIWMKVKLLGANSNAFDSWLLLQGMKTLEIRMQRHC
                     DNAAVVAAFLSDHHAVKKVHYCGHKDHPQRNIINRQMSDYSGMLSFELKGGLKAGKKL
                     MQSVKLCNVITTLGTLDTLIQHPASMTHVNVPKERRLAAGISDGLVRMSVGIENAADI
                     LNDLEQGMK"
     gene            7969..9234
                     /locus_tag="PLL28_08010"
     CDS             7969..9234
                     /locus_tag="PLL28_08010"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNF69308.1"
                     /translation="MTYRYSDNSLPMVLIIGAGFAGLELIKQFNNKPFRVLLLDKNNY
                     HTFQPLLYQIASAGLTAESIAYPVRRKIGAYPNIAFRVAEVSSVDTSGQLVHTDIGEF
                     HYDYLIIASGASTNFYGNNSLASTTFPLKSVQDALNMRTTILQQFEKAVNASKTNGSK
                     LLNFIIVGGGPTGVELAGALAEIKKNVVPSDYREIDPEMMEVHLIEAAPRVLGTMSEK
                     SSRLAKKYLEQLGVKVWLNAVVNSYENDILTLSNDTKITAHHVIWTAGVKGVDIKGLE
                     GNIRTRGNRIITDRYHRISGFENIYAIGDIASIQGDEQYPQGHPGVAQVAIQQAHNLA
                     SNFIRNAKGKSIQAFNYHDKGSMATIGRHKAVVDLPFISFGGYLAWYIWMFIHLMALV
                     GFRNRFMVFMNWMWNYITYDRALRLILKK"
     gene            complement(9291..9944)
                     /locus_tag="PLL28_08015"
     CDS             complement(9291..9944)
                     /locus_tag="PLL28_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69309.1"
                     /translation="MRKLIFAICATLTGLFAFQSASAQLWLMTDGGVFFTKNLVGEGI
                     NAKAAWHFGDYDHNMVTFGGGYNLLGSSKVDIIVVDTAADVTDTIESTLTSNVITIDG
                     DYRRYFLGSDADDYFAFYGLGGLSLWMVNTKVKMGSYVDTVYMVEEGTKLVSSNMSVR
                     IPVGFGVDWTVRGRLWWYVEAKLELPITQVNNDFIGNDFGVSYHINTGARILLWDLF"
     gene            10098..10802
                     /locus_tag="PLL28_08020"
     CDS             10098..10802
                     /locus_tag="PLL28_08020"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNF69310.1"
                     /translation="MAKDTIIITDADGGLGSSVTSALLKAGYMLRASAMHEEAVERLK
                     SSYPEHYGNALTAVIADLGTEEGVKALLEGIPQPSGLVHLAGGYKPGNAVSDYSNDDF
                     SFLMNLNARPTFLLLKHLMPSLKALGKGSIVTIGAKPALHPVAANAVYTASKSAVIAL
                     TMAAAEEGRPFNVRANCIIPAALQTPGNLSWASKEQFETFTPLQDIADTILFLMSDEG
                     RGITGTTIPMYHKIAT"
     gene            10830..11546
                     /locus_tag="PLL28_08025"
     CDS             10830..11546
                     /locus_tag="PLL28_08025"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNF69311.1"
                     /translation="MLKDLHPEQLFFFDIETVPGAADYNELAPDLQQLFERKVGRQRN
                     EDEDPAEFYFSRAGILAEFGKVICISCGYLQKKENEYHLKIKTFYGDDEKSLLEEFTQ
                     TLLNISSKGYQLCGHNIREFDVPWLCRRMLINGLILPPVLDIYGKKPWEVNHVDTLDL
                     WKFGDYKHYTSLNLLATILGVPTPKDDIDGSMVAEVYYKQHDLNRIAVYCSKDVVAVA
                     QVIMKLKGLPLIKSENISGI"
     gene            complement(11616..12047)
                     /locus_tag="PLL28_08030"
     CDS             complement(11616..12047)
                     /locus_tag="PLL28_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNF69312.1"
                     /translation="MTLVKKGWSSPFSNKFLSDFFNADKLFEDDFFNVERMPAVNIVE
                     SADHFEVEVAVPGMKKEDFKVEVKDGMLHISAEREENKEEVKKNYTRKEFSVASFSRM
                     FTLPENVKDVDIGGEYKNGVLHIVLPKKEPVKIDAKKIEIK"
     gene            12224..13213
                     /locus_tag="PLL28_08035"
     CDS             12224..13213
                     /locus_tag="PLL28_08035"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HNF69313.1"
                     /translation="MRSFLAMTFMLFLACPVFAGSDSLHAHIALPLPDKGIDDPIPQG
                     DFEVLVIPLKRVQNLYMVEAKVDSVVGNFILDTGAPHLVLNKTYFNTGKSAPGSTQYG
                     ITGGGNSVMNTTIDTLKVSELFYTRVDADIVNLGHLENARGVKILGLLGTSLFSRIEM
                     EIDMKNDLLYLYKTDEKGERLIPNNHTTPPDMQMPLDIVNDVLFIEGRIANKKLRFCL
                     DTGAERNVLSNTVSNKIISRFTLTKSSNLAGSGNSGQVVLNGVIDSVVTEGVSFTDMP
                     FILTNLSYLELVYGTTVNGVLGYDYLSKGYAVINMQKKLFSMYFYKPEEDDEP"
     gene            13200..14642
                     /locus_tag="PLL28_08040"
     CDS             13200..14642
                     /locus_tag="PLL28_08040"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HNF69314.1"
                     /translation="MMNHNLSRILIFLMLLFPTVSNAQFAGSTVQVKGDMIIIRIDQK
                     NNDYNNVMLYFGLNEDSLFEYGNIGPLAAQGWELLDLTKRTAKIGRLITSSSSDIYWG
                     NQPFVINFPDVSNTMPGYPGDVVYGANSFIHKPSVFEDHSGNTVFYLPQHLNASTVYL
                     SGNFNDWSTGGTPMTKTDSGWMYSMKLEPGKYFYKFIIDGNWIHDVNNALRESDGHDG
                     FNSAYYVNNTVIRLKGFTDKKDIIFTGSFNNWNEHELHMIKTAGGWELPMFLKEGTYT
                     YKFIADGEWMLDPSNEIARPDGNGNVNSVMSVGDTTFFVLNGFEDAKIVILTGSFNNW
                     NTGELIMQKTSGGWQIPYVLAAGNYTYKYIVDGTWITDPSNPITSGQGDEINSVRTIK
                     PNHVFKLADYPTATNVYLSGSFINWAEPGFKMTKVDGVWKIPVYLPPGKCTYKFIVDG
                     NWITDPANPQTEQNEYGTGNSVIWKEVNAQ"
BASE COUNT         4391 a         3104 c         2985 g         4164 t
ORIGIN      
        1 ccgcatgtaa atggtgtccc acatcaccaa atgtcaacac ttcaccctcg ggtaacatga
       61 gttgcatata ccctttttgc atgggtttca ggtttcgtaa tatggttgat ttataaaaac
      121 tgttcatata tagttctcgc ttcgggtaaa caataatgaa aatatacgca tttattttta
      181 atcaggttgt ccactcttgt ttaaaatatc gcgttgtagg cttgcctgct cggttttccg
      241 atgccatgga acctttttca gccataacaa aaacgcatgc caatgaatag ccccgatcac
      301 ctgaagggta atgaagggaa acctgaccac ataccataac aatcggaggt ctgataatgt
      361 tcgacgggca ccggtgagcg tgctgatgaa gaagcgatcg ccacttctgt cttgtacatc
      421 gatgcggaga ttcagcttat cagagggcag ttgatattta aaccggaagg aggtatccat
      481 atcggtgaag ggagaaacgt aaaaatattt cacatcttcc tgctcaaatg attgttgttg
      541 cttttgatcg ataagaaata ttttcatctc cccaaaagta ttgctgatct ccgttacaac
      601 atacaagcaa tcgcctgatg cattgtaaca aaaataaaaa ctcaccggat tgaatacata
      661 gccaaacata cgaatatgtg tgagtaacat caccttttcc ggcatatcaa caacaccatt
      721 ttctttcaac caggcattca gttttgctct gaccgtcaac tcattccggt tatccccttc
      781 aggatacttg aagtgatcgc gatctcggaa tgaaaataca cctcctttat tataggataa
      841 taagcgatgc gactttgcaa tttctgcact gttatctata tcccaaaaaa acataaatac
      901 cttatagtta aacctatgct tcttcggaga aagccgatta tgcatcacta agcaggtata
      961 taatgttgaa ggtgtcatta tttgataaat ttacgaaagc agtttgtatt ataattaaat
     1021 cactttcctg cccaatattt gttcacatac atttgccgca ctcatcaagg catcttcatg
     1081 aaatccatag cgaaaataac ttccacagaa ataaagactg ccattccggt ttaattcagg
     1141 gagtttcgcc tgtgctttca tagcctcgac agtgaataca ggatgctcat aaacgatctc
     1201 tttgataacc ttatttccat ctatgatgcc agggtcattt atcgtgacga aataatcagt
     1261 aaaatcggag acatcctgca gactattcat ccaataagtg caagaagaga ctaagttatt
     1321 tcccctcgtt tgagttctgt aattccatga gctccacacc atttttttct ttggcatgat
     1381 actgccatct gtatgtagtg ttgcgacatt tttctgataa ctgaatggcg ataacaatgc
     1441 cctttcctct tgtgatgcat cgctccacat tttcaatgcc tgatcgccat gggcagcaac
     1501 gattacttta tcgaactgaa agacacttcc gtgcacatct gtcaattcga caccatcact
     1561 tgttctacgt atcgagcgga taccctgatt cacaaaaata cgctccttaa atggcgctat
     1621 taatttttgc ttatactgct cactacctcc tgaaaccgtg taccattgga actgcgtatt
     1681 caatcccaaa aatccatggt tagaaaaaaa tcgcaccagg cttagtacag gaaattgtgc
     1741 agtagtatcc ggaggagtgc tccacaaggc cgaactcata ggtaagatat accaattcag
     1801 cagatcatct gaatattttt tcattcgcag ataatccttt atcgataaag ctgagagcgt
     1861 ttcattatga aaaacttcta tacaagtttt attgaaagtg tttaactgtt tgagaaagcg
     1921 aatatatcga ggcgaaaaaa ttcttcgtcg ctgtgcaaac aatttattga gactactgcc
     1981 gcaatactcc aaatgctcat cgcaaatctg cgcagcaaag ctcatatccg tttttttcac
     2041 ttttacctgc aatgcatcga acaaggcagt aagtcgcgga taagtatgat tattaaacac
     2101 gataaaaccg gtatcaatcg gaatgtcctg acccggttcc tccacaaaaa cggtattggt
     2161 atgaccacca attctgttat cagcttcaaa tacagtgatt tcgtgatcgc gatgcaagta
     2221 ccaagcggcg cccattccgg caataccggt tcctattatt gctatgcgcg ccatatcaaa
     2281 cagtcgcttt tttggtaaaa agataatggc tcactatcca ttcgcggcca ttattgaaat
     2341 tccaaagttc ggcgcaggcc ataaaaaata tgcgccaata gacccaccat ttcaccgatt
     2401 gttccgcacc atatgtttct gcaagaatgg gcattatttc atttttatgt gcatccatat
     2461 tcgacaacca cgcttcggaa gtttttccgt aatgtgtgcc atcccaatgc cagtgatctt
     2521 gaatggtgag atgatcgtta aaataaaata aaagatcgtc ggaaggcata acaccgcccg
     2581 taaagaaata tttactcatc cagtcggtat catcaaccac atcgaagaaa taagtaaatt
     2641 ctttgtgtgt gaaaatatgt acaaataatt ttccatcagg cttaagaaat cgcgaaattt
     2701 tttcaagcag caattgataa ttccgcatgt gttcaaacat ctccactgaa accacacgat
     2761 caaaagtttc attgatgtcg aacacattca tatcggcagt aataatgcgc acattcgtaa
     2821 ttccgcgcat tttacattga gattctatat gtaatttttg ggtagacgaa ttggaaacac
     2881 ctgttacatg tgccgaagga aatttctgtg ccataaacat ggttagactt ccccatccac
     2941 aacccagttc aagtatgttc atcccttcgc gcagctgagc tcgctgacaa gttatttcaa
     3001 gcgcctttgc ttcagctgat gacaggtccg gtgtattatc atcccaatag cagcacgaat
     3061 atttcatatg ctttcccatc accatactga aaaaggcagt tggcacttca taatgctgtt
     3121 cgttggcagc tccggtttcg atggcgatgg gcgaggcctt caattggcca atcaattcca
     3181 tcagatgcaa ttgttgttgt tcagcggtag aaaatgattc atccttcagt cgccggcgaa
     3241 gcaaggaacg aatccgtgca cgaatcaaga aatcaggaat cctgtcgcgt tcaagtaaag
     3301 tattgatcac agttttgggt ttcttatgcg ttaagaatga ttcaaatata cgggaacaag
     3361 aagttaacag atgtttcttt cccggtgatt gaaaacagaa aagaactact atggttcagc
     3421 aaattcggct attcaaaggg atttcgcgca ggaattggta tataaattgc acgttaaaac
     3481 atacaaacca caagcaatta tgaacgccaa tgtgcatttc gtggttgtag aggtgaacat
     3541 ttcgtaacaa ttttacgtat ataataccat aaccttttaa cccaatatcc tcagccatta
     3601 tggtaaaaca attcggctta cccaattatg tgatctggct cttcggcggc attgcctttg
     3661 ttgccgttgg cactatttca gtttttcatt ggacctatgg gcttgcaaca tttattctga
     3721 caacgggagt tgccttcgcc ggatattggt tctttttccg tcccgccttt gtggcattgg
     3781 acattgcacg caagggagaa cctgccatgg ctattattct gcaagtatgg gaaacaggtc
     3841 gcaccgttaa cgacaatccg cgaatcaaac ttttattgga gatacgtcac agcactatgg
     3901 tgccttatga agttgaagtg cgccaaaata ttgctaagga cgacatgcgc ctttatcagc
     3961 ccggtcgcac gctacaagtg aaggttgaca gtcgcaaccc gcgaaaagtg gctattcttg
     4021 ctgcctgagg ccggtcaaat tcactgattt tattgtattt acccgtatct ttgcgccatg
     4081 gtttacctta cacgtagaga aacctttaat gccgcgcacc gtgtgtacaa caatgcatgg
     4141 agtgaggaga agaacaaaga gatgttcggc aaatgcgcga ataaaaactg gcatggacat
     4201 aactatcagt tatttgttac cataaagggt aaaccagatc ctgacaccgg ctttattatg
     4261 gatgcaaaga agctcagcga tgttatcaaa agagagatcg tagagcgttt cgaccatatg
     4321 aacctgaata tggatactcc ctatttcacg gatcagcaac ccagcaccga gaacttttgt
     4381 gtggtgatat gggcgcttat tgaacctttc atcacaggtg gtgttttaca ctgcatcaga
     4441 ttacaggaaa ctgaaaatat ctatgcagaa tactacggtg aacaataaca agcgcatctc
     4501 agatgccgac gatgagttca acatttcaga cagacactat accaaaatcg attcttacgg
     4561 catggaaacg ctcgacaatc taatccatca ttaccaaggt attatcactt cattaggtga
     4621 agacgccgaa cgcgagggac tggtgaaaac ccctgaacgc attgcaaagg ccatgcaatt
     4681 tcttacacag ggttacaatc aggatgcaga agcggtgatt agaagtgcca tgtttaacga
     4741 agagtgttct gaaatggtaa tcgtaaaaga tatcgagctg tattccatgt gcgaacacca
     4801 tatgctgcca tttttcggtc gtgctcatgt ggcatatatt ccaaatggaa aaattgtagg
     4861 actcagtaaa attgctcgcg tagtagatat tttttcccgt cgtctgcagg ttcaggaacg
     4921 tcttacacat caaattctgg aagcactgca aaataccctc aatccacatg gtgtagctgt
     4981 tgttattgaa gccagccatt tgtgtatgat gatgcgtggc gtgcaaaagc aaaattctgt
     5041 aacaaccact tcagcattta taggccaatt tcaggaagtc caaacccgca acgaattcat
     5101 caatttgata gctgcaaggt taagtaaata aggaattcac ggcgtttccc attgatatag
     5161 gtctttcttg tttctttacc ataggtccca actatggcaa tcctgcattt tgttgttact
     5221 tttactgcga aattgaatgc tatgcctaat ctccgttcaa caatactgat gctgtttatc
     5281 tgcagcggtt taattgccca agcacagcca aaacaggtta aactgaaagg tcataaatgt
     5341 ccatctatag ccataagcga ccccggtgaa ttttatagca catacaattt ctctgataat
     5401 gatttgcatg cgttagattc ggcaggatta aaaagtacgg tgatcgaaaa tattattgcc
     5461 catcataccg aaacaagctg gcccggaaaa ttagcaagcc tcgatgagcg tattgcaaat
     5521 ccggacatac tgaaatcata tgtagtgtat aaggttgcag aaataaataa tatggttgtg
     5581 gtactcgtcc ccgctaaata taatagcgac aaaccggaag gctgggcatt gactgaagat
     5641 gtatatttta ttttgagtaa agcaggtgta aatctctaat caacaattga tatgaaaaaa
     5701 gcatacgtat ttcccggaca aggttctcaa tttccgggca tggggaaaga tctttatgaa
     5761 aacaatgaag ctgcccgtgc attattcgag cgcgctaatg agattctcgg gttccgaata
     5821 acagatatca tgttcaatgg aaccgaagaa gaactcaaac aaacaaaggt aactcaacct
     5881 gcaatattcc ttcactctgt gattttggca tccacattat ctgattttaa tcctgatatg
     5941 gttgccgggc attcacttgg tgaattcagc gcattagttg ccaatggtgc tttatcattt
     6001 gaagatgcct tgcgattagt atcggctcgc gcacaggcca tgcaaaaagc ttgcgaatta
     6061 caagcatcaa ccatggcagc agtattggga ctagcagatg aaattgtcga gaaaatatgt
     6121 gcagaaacac cgggaattgt tgttgcagcg aattataact gtcccggaca attagtgatt
     6181 tcaggagaag tagaagcagt aaataaagct tgtgaagcat taaaagctgc cggagcgaaa
     6241 cgcgcattat tgttacctgt aggtggagct ttccattcac ctcttatgga acctgctcga
     6301 attgaattga gtgctgctat tgaggcaaca ccatttaata cacctcgctg tcctgtatat
     6361 caaaatgtaa ctgcaagcgc aattattgac cctgcagaaa taaaaaataa tttgctgatt
     6421 caattaactg ctcctgttaa atggacacag agtgttacac aaatgactgc agatggcgct
     6481 acacattata tcgaggttgg tccaggaaaa gtattgcaag gtcttgtaaa gaaaatcagc
     6541 cctaccgcag aagttgccag cgcctaattg atgcaaatga tgctgcttcc ggcaataaaa
     6601 aatcagactg ttgtttttga taacagtcca aatcccattg catcatttca tcccttgttc
     6661 caggtcattg agaatgtctg ccgcgttttc aattcccaca ctcattctga caagaccatc
     6721 agaaatccct gccgccagtc ttctttcttt gggaacattg acgtgcgtca tacttgcagg
     6781 atgctgaatc aatgtatcca gtgtgccgag tgttgttatc acgttacata atttcaccga
     6841 ttgcataagt ttttttccgg cctttaatcc tcctttcaat tcgaaactca acatcccgct
     6901 gtaatcagac atctgcctat tgataatatt gcgctgagga tgatctttat gtccgcaata
     6961 atgcaccttt ttcactgcat ggtgatccga taaaaaagcg gctaccacag cggcattatc
     7021 gcaatgtcgt tgcatgcgga tttccaaggt tttcattcct tgcaacaata accatgaatc
     7081 aaatgcatta ctgtttgcgc caagcaattt cactttcatc caaatgcgct cccgcataaa
     7141 atttctatcc ctacttacta ccacaccact aagtccatta ccatgaccat tgagatactt
     7201 tgttgctgaa tgaattacaa gatcagcgcc gagtaataaa ggcttttggc aatacggaga
     7261 agcaaatgta ttatcaacag ccaccatacg accataacga tgtgcaattt cagcaacagt
     7321 ttgaatatct gttaccgaca tcatcgggtt ggagggtgtt tcaatataaa caagtgtgat
     7381 ttttttattt ttcttcaagg ctttttcaac agcatcaaaa tcattcaaat ccatgcgcat
     7441 ggtattgata ttccaggctg caagttgctt ttgcatgagt tcatcggtag taccatataa
     7501 ctgatgttgt gttaatatag tatctcctga agttgcgcag gaagtcattg cagcagcaat
     7561 tgcagccatg ccgcttgaaa acaattgagc ggtagcatca atatcgctgc ctgatgtttc
     7621 cagcaaggca attttttttt ctgccatttc tatggtcgga tttccccatc tggaatacac
     7681 atagcctttt gatgggtcat cgaaaagcat attggcatga tcaagtgatt caaatccata
     7741 ggcggttgta gcgaatatag gctcgttatg cgagagaatt ttcctctcag tagccattgg
     7801 ctgcacacaa atggagccga aaccttttgc ctgttttttc tttgccatac ggattgaaaa
     7861 tatgccgtta aaattaggtc attatttact gacgaaacat aaaaatatac acattacatg
     7921 aaccaacgcc cggcgagtac ggttatcttt gcagacaaat tcaagtatat gacctatcgc
     7981 tacagcgata acagtcttcc gatggtgctc atcattggcg ccggtttcgc cggtctcgag
     8041 ttgattaagc aatttaacaa caagccattt cgcgttctgc tgctcgataa aaataattat
     8101 cacacctttc aaccactgtt gtatcaaatt gcatcggccg gcttgacggc agaaagcatc
     8161 gcatatcccg ttcgccggaa aattggggca tatcctaaca tcgcttttcg tgttgcagag
     8221 gtatcgtcgg tagatacttc ggggcaattg gtgcataccg acatcggcga atttcattac
     8281 gattatctca tcattgcttc aggcgcaagc acaaattttt atggtaataa cagtttagca
     8341 tcaacaacat tcccattgaa atcggtgcag gatgctttga acatgcgtac gacaatttta
     8401 caacagtttg aaaaagccgt taatgcctca aaaaccaacg gcagcaaatt attgaatttt
     8461 attatcgttg gtggcggacc gacaggagtt gagcttgcag gcgcacttgc cgaaatcaag
     8521 aaaaatgtag tgccatcaga ttaccgtgaa attgatcctg aaatgatgga agtgcatttg
     8581 atagaagctg cgccgcgagt gctaggtacc atgtctgaaa aatcgtcgcg actagcgaaa
     8641 aaatatcttg agcaattggg tgtgaaagta tggcttaatg cagtagtgaa ctcatacgaa
     8701 aatgacattt taaccttgtc taacgacaca aagattaccg cgcatcatgt aatatggaca
     8761 gcaggtgtta aaggagttga tatcaaaggg cttgaaggaa atattcgcac tagaggcaat
     8821 cgtataataa ccgataggta tcatcgaatt tccggatttg aaaacattta tgccattggt
     8881 gatattgcga gcatccaggg tgatgaacaa tatccccaag gacacccggg ggtggcgcag
     8941 gtggcaatac agcaagcaca taatcttgcc tcgaatttta ttcgaaatgc aaaaggaaaa
     9001 tctatacagg catttaacta tcacgataaa ggcagtatgg caacgatagg ccggcacaaa
     9061 gcagtagttg atttgccatt tatttctttc ggaggatatt tagcgtggta tatctggatg
     9121 ttcatccacc tgatggcatt ggtaggcttc agaaaccgat ttatggtgtt tatgaattgg
     9181 atgtggaatt acattacgta cgacagagca ttgcgactta tcctaaagaa atagcaatct
     9241 ctgtcgcgtt gcgattagtt gttacccaat aaatcaagct gatccgggaa tcagaacaag
     9301 tcccaaagaa ggatacgtgc gccggtattg atgtgatagc ttacgccaaa atcgttgccg
     9361 ataaaatcat tgttcacctg agtgatcggc aattccaatt ttgcttcaac ataccaccaa
     9421 aggcgacctc taacggtcca gtcaacacca aaacctacag gtatgcgaac actcatattt
     9481 gaagacacta atttagttcc ttcttcaacc atatatactg tgtctacata tgaacccatt
     9541 ttcactttgg tattcaccat ccataaactt aatccaccga gaccatagaa tgcaaaatag
     9601 tcgtcggcat cagaaccgag gaaataacgg cggtaatcac catcaatggt aattacattt
     9661 gaagtgagtg tagattcgat ggtatcagta acatcggctg ctgtatccac aacaataata
     9721 tctactttag aagaacccaa taagttataa ccaccaccaa aggttaccat gttatgatcg
     9781 taatcgccga agtgccaggc agcttttgca ttaatacctt cacctaccag gtttttagtg
     9841 aaaaatacgc ctccatcggt catcagccat aactgggcag acgcagattg gaatgcaaat
     9901 aatcctgtca gagtagcgca aatggcaaag attaattttc tcatatgggt gttgtttgac
     9961 attccccgcc catgggagac ggcagaataa gcacaaaata aaggtttttt accctatttg
    10021 cgaaattccc cacccacaat aagggacact aatgtccaat catgcacaca atcaggcgaa
    10081 tatgtaaatt tgagcctatg gcaaaagata ccattatcat tacggatgcc gatggcggac
    10141 tgggaagcag cgtcacttct gccctgctta aagccggcta catgctgagg gcaagtgcca
    10201 tgcatgagga ggctgttgag cgactgaaaa gcagctatcc ggagcattat ggcaatgcgt
    10261 taacagcggt gattgctgat cttgggactg aagagggtgt taaagcgctg cttgagggta
    10321 ttccccaacc gtcgggactg gtgcaccttg caggagggta caagcccggg aacgccgttt
    10381 cagactattc aaacgacgat ttcagttttt taatgaattt gaatgcaagg ccaacatttt
    10441 tgttgctcaa gcatctcatg ccaagtttaa aagctttggg aaaaggttct attgtaacta
    10501 ttggtgccaa gcccgcacta catcctgttg cggctaatgc tgtgtatact gccagcaaaa
    10561 gtgccgtaat tgcattaaca atggcggctg cggaagaagg aagacctttt aatgtgcgtg
    10621 ccaattgcat cattccggct gctttgcaaa cgcccggaaa tttaagttgg gcctctaaag
    10681 aacagtttga aacattcacc ccattgcaag atattgcaga tactatttta ttcctgatgt
    10741 cggatgaggg acgcggaatt accggcacaa ccataccgat gtatcataaa attgctacat
    10801 aattttttca tcattgttta ttccttttta tgcttaaaga cttacaccct gaacaactgt
    10861 tttttttcga cattgaaaca gttcccggtg cagccgatta taatgagctg gctccggact
    10921 tacaacaatt atttgaacgc aaggttggcc gacaaagaaa tgaagatgag gaccccgcgg
    10981 agttctattt ttcacgcgcc ggaatacttg ccgaatttgg aaaggtaatc tgcatctcat
    11041 gcggatatct tcagaaaaaa gaaaatgaat atcacctcaa aataaaaacg ttttatggtg
    11101 atgacgaaaa gtcactgctg gaagaattta cgcaaacatt gctgaatatt tcttccaaag
    11161 gctatcaatt atgcggacat aatattcgcg aatttgacgt gccatggctt tgcagaagaa
    11221 tgcttatcaa tggtttaata ttgcctcctg tgcttgacat ttatggcaag aaaccatggg
    11281 aggtcaacca tgtggataca ctggatctgt ggaagtttgg cgattataaa cattacacct
    11341 cactcaattt gctggcgaca atactcggtg taccaactcc aaaagatgat atcgacggaa
    11401 gcatggttgc cgaagtatat tataagcaac acgatctgaa tcgcatagct gtttattgca
    11461 gtaaagatgt tgttgccgtt gcccaggtaa ttatgaaact caaaggttta ccactcatca
    11521 aaagcgaaaa tatcagcggc atttaaaaaa tacggggcga tgtgccatca agtacatcgc
    11581 cccgttacat acctcagaac agactacgcc gacgcctatt tgatctcaat ctttttggcg
    11641 tcgattttaa caggttcttt ttttggaaga acaatatgga ggacaccgtt tttgtactcg
    11701 ccgccgatgt cgacatcttt aacattttca ggaagtgtga acatgcggct gaaggatgct
    11761 acgctgaatt ctttgcgcgt ataatttttc ttcacctctt ctttgttttc ttccctttcg
    11821 gcagagatgt gcaacatgcc atcttttacc tctactttaa aatcttcttt tttcattccc
    11881 ggaacagcca cttctacttc gaagtgatct gcactctcca caatatttac agcaggcatt
    11941 ctttcaacat tgaaaaaatc atcctcaaat aatttatcgg cattgaaaaa atcgctgaga
    12001 aatttattgc tgaatggaga gctccagcct ttttttacca gtgtcataac tcagttttta
    12061 agtggtgaac tgaatattgt tgacattgca attataccca cataaacgaa gcaaaaacag
    12121 tgatgttggt taccgaaaat tgaaattgta atcaattagt cgcccggtaa tccccgtcac
    12181 cctcggcaaa gagcatctta aaccgtattt ttgcccatag ggtatgcgca gttttcttgc
    12241 catgacattc atgcttttcc ttgcctgtcc ggtgtttgcc ggctcagatt cattgcatgc
    12301 gcatattgct ctcccattac ccgacaaggg cattgatgac cctattccac agggtgattt
    12361 tgaggtattg gttattccat taaaacgcgt tcaaaatctt tatatggtag aggcaaaggt
    12421 ggatagcgtt gtaggcaatt tcattctcga taccggagcc ccgcatttag tgctgaacaa
    12481 aacatatttc aataccggaa aatcagcacc gggttctaca caatacggaa ttacaggagg
    12541 tggaaattca gtgatgaata ctactatcga caccctaaag gtcagtgagc tgttttacac
    12601 gcgtgttgat gcggatatcg ttaacctcgg acatcttgaa aatgcccgtg gggtgaagat
    12661 tctaggtcta ttaggcactt ccttattcag ccggatagaa atggagattg atatgaaaaa
    12721 cgacctgctc tatctgtaca aaaccgatga aaaaggtgaa cgcctcatcc ccaataatca
    12781 cactacgcca cccgacatgc aaatgccact tgatatcgtc aatgatgtgt tatttattga
    12841 aggtcgcatt gccaataaaa aattgcgatt ttgcctggat accggtgcgg aacgaaatgt
    12901 gctcagcaat acagtaagta acaagataat tagccgattt acccttacca aaagcagcaa
    12961 cctggcaggc tcgggcaaca gcggacaggt agtgttgaac ggtgtgattg attctgttgt
    13021 tacagaaggg gtgtctttta cggatatgcc attcattcta accaacctta gctaccttga
    13081 actcgtatat ggtactacgg ttaatggagt actgggttac gactacctgt cgaaagggta
    13141 tgccgttatc aatatgcaga aaaaactttt ttccatgtat ttttataaac cggaggaaga
    13201 tgatgaacca taatttgtcg aggatattaa tctttctgat gctgcttttt ccaacagtat
    13261 caaatgcaca atttgcaggc agtactgtgc aggtaaaagg cgatatgatc attattcgca
    13321 ttgatcagaa aaacaatgat tataacaatg tgatgctgta tttcggttta aatgaagata
    13381 gtttattcga atatggcaat attggacctc ttgcagctca aggatgggaa ttattggact
    13441 tgactaaaag aacagcaaaa ataggtcgat tgattacctc atcaagcagt gatatttatt
    13501 ggggcaatca accctttgtt atcaattttc cggatgtgag taatacaatg cccggctatc
    13561 cgggtgatgt ggtgtatggc gctaattcct ttatccataa accaagtgtg tttgaagacc
    13621 attcaggcaa tactgtattt tatcttcctc aacatctaaa tgcttcaacg gtatatctca
    13681 gtggcaattt caacgactgg agcaccggtg gaacaccaat gacgaaaaca gattccggtt
    13741 ggatgtattc catgaaattg gaacccggaa aatatttcta caagtttatt attgatggaa
    13801 actggataca tgatgtaaac aatgcactcc gcgaaagcga tggtcacgat ggatttaata
    13861 gtgcgtatta tgtaaacaat accgtgatca gattaaaagg atttacagat aaaaaagata
    13921 tcatattcac aggttctttc aataactgga atgagcatga actccatatg atcaaaactg
    13981 ccggcggatg ggagctgccg atgttcctga aagaaggaac ttacacatac aaattcattg
    14041 ccgatggcga atggatgctc gatccctcaa acgaaatcgc cagacctgat ggcaacggca
    14101 atgtgaattc cgttatgtcg gttggcgaca ctacattttt tgtgttaaat ggttttgagg
    14161 atgcaaaaat tgtaattctt accggcagct ttaataattg gaataccggt gaactcatca
    14221 tgcaaaaaac ttccggtggt tggcaaatac catatgtgct tgctgcggga aattatacct
    14281 ataaatatat tgtcgatgga acatggataa cagatccgtc aaatccaatt acttccggtc
    14341 agggcgatga aataaactcc gtgcgcacaa tcaaacccaa tcatgtattt aaacttgccg
    14401 attatcccac agcaactaat gtatacctat ccggaagctt tatcaattgg gcagaaccgg
    14461 gcttcaaaat gaccaaggtt gatggtgtgt ggaaaatccc tgtttacctg ccacccggaa
    14521 aatgcactta taaatttatt gtcgatggca actggatcac cgatccggca aaccctcaaa
    14581 cagaacaaaa cgaatacgga acaggaaatt cggtgatatg gaaggaagtc aatgcccaat
    14641 gatt
//
LOCUS       DAOQHY010000080        14345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2021_length_14345_cov_6.013296, whole genome shotgun sequence.
ACCESSION   DAOQHY010000080 DAOQHY010000000
VERSION     DAOQHY010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14345
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2021_length_14345_cov_6.013296"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(472..2097)
                     /locus_tag="PLL28_08045"
     CDS             complement(472..2097)
                     /locus_tag="PLL28_08045"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69315.1"
                     /translation="MQDIRINTRALRLTILMMLLFFTRFVFAQDGELDDSFGEGGLVK
                     TNLPVTDELCADAVVDADGFITIAGSAIQGGVRKALIARLNPDATPDVLFNETGCEVL
                     SDDGEIEESQVNAIALQSDGKIVAGGYVTFLGDTYGVLYRCNQNGGLDTTFGNSGIAK
                     FFSSYDQNYAQVKDIAVQEDGKIVFCGIEHSENTGFIDQFVTGRCLPDGTPDSSFGGA
                     GLVYCYFDWISGSAATIQVQTDGKILVGGRTNSDFALIRYLPNGVPDNTFGEWGEVTT
                     DIGYDDFITDLGILSDGKIIAVGYSKLPSSGNCYYKTRTSIACYNVDGSLYDGFSNDG
                     KLEMFFNYCRDEAYSVLIQPDDKIIIGGGTGKQNNSDSYNYYFLRLHKDGTRDYDFNS
                     DGYLESDMYENNYYDSYAKALLFQPSGKAVLVGYTDHWSISDSFISAIRVTTGVDSVA
                     LAQMNVYNQSSSDSTFSVWFSANLLYVKNVSTESIRLTICNISGSKLYTFQAESGMSN
                     YSLRNLPKGMYFVTGEDGKNVIAQKFIVSYAGY"
     gene            complement(2084..4105)
                     /locus_tag="PLL28_08050"
     CDS             complement(2084..4105)
                     /locus_tag="PLL28_08050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69316.1"
                     /translation="MNEKLKKLIQYSSHAGAFLALPAGLHGQVIYHNIDPDSVFSNDG
                     HVDIDFNADGMNDLTITEYYYYDLTFWTDYTMAWWGDFSVQISNFSGVISNVSYPSSY
                     GAMNLIEGFSINSAANWNTLDLIKLIHFSSYDSFIYAFNYDNWKGLNKYIGVKFLIGS
                     DTHYGWVRLGISSNGSVEGVKSYSLAVQDFAYEATADVPLLISNPTASVAEHVFISDV
                     YENENASDFRVQFDNAAYESTVSAYRIFLCPYTYAHPTPPSLAFLETLPPERYMEVEA
                     IGTPTYDVVLPATMTDINGAPVLGNYPHYYRALIVSMADGVMVSQNNVSLPSNTIYTK
                     LTAVPSPYGLSIDKPGSNCDITDFQVSFHKAGDESYISEYRVYILNDDHDIIAENDPS
                     YYVSVVPDGSLTYTVTPDVNTLIFEDTVPILFQNYYTEIMTVGDSIHASVASFESSRY
                     TSTYYGSTIGIEVELYCEQYDHTPVISLVDFTKTPADIRVQFNGPTKMSGVGQYRIYI
                     VPEEDIPNFTPEIAEHVPWISYTKIGDPEPYLDTVLSAELLDINGHTIALDKRYAVMI
                     GLFESSAPQKIAISLPSETFSFLSDESNIPLPYSYIYDNELYVISDINLPYMLNLYNM
                     AGEKVFTYEITSPITIIDLSDLPKGLYIIKGTTADEMPATKIFVGHAGY"
     gene            4380..4610
                     /locus_tag="PLL28_08055"
     CDS             4380..4610
                     /locus_tag="PLL28_08055"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNF69317.1"
                     /translation="MWQEENNQLHKTFMFHNFSEAFAFMTRVAMLAEQHNHHPYWTNV
                     WNKVEIFLNTHDAGNIVTEKDHALAKAIDALL"
     gene            4633..6747
                     /gene="ligA"
                     /locus_tag="PLL28_08060"
     CDS             4633..6747
                     /gene="ligA"
                     /locus_tag="PLL28_08060"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNF69318.1"
                     /translation="MKYDQAAQHRLIALTEQLVKNLSDKNTNADTARDTIDDLKQVLH
                     FHDWKYYVDASPVIRDIDYDLLFKKLQSLEKSYPELLTDDSPTQRVARTLSDDFPTVS
                     HSVPMLSLENSYDENDLNTFDKRVRELTSTDNIIYCVEPKFDGSSIAVIYEGDILVRA
                     ATRGDGIQGDEITINAKRMRSLPLRADFSKYGIHKVEIRGEVVIGKETFARINEKRES
                     EGQQILQNPRNSAAGALRVKDSEEIERRGLEAFMYHIAYAVDKDGNDLLGDQLRYHFD
                     NIEMLGKLGFKIPGKEKKKCHSVEEVHAFVQQWEQDRDDYTYEIDGMVIKVNDIAQQK
                     ICGATSHHPRWAIAFKFKAREQETELLSVEYQVGRTGAITPVAKVKTVYIGGVNVSSI
                     SLHNEDIIKEKDLHLHDIVVVQRAGDVIPYIDRVVLAKRDNSKIKTIDFIKECPSCNQ
                     AIYKPEEEAVYRCINPECPAQMEERLIHFVSKDAMDIDGCGRETISELYQAGKITTIP
                     DIFDLKTSDLAGREGWKEKSIAKLIDGIEASKKQPLWRLVNGLGIRHLGTQTAKDLVK
                     NISDLTELFDWDLERYQAIEGIGPKVAASIHQYFSNAFNQQMILKLKEDGLNMANEKT
                     AGGSGKLSGKTFLFTGSLTRFTRDEAKELVEANGGKLIGSVSKNLNYLVAGESAGSKL
                     DKAKALGTVEIIDEEAFLAMIA"
     gene            6972..7223
                     /gene="rpsT"
                     /locus_tag="PLL28_08065"
     CDS             6972..7223
                     /gene="rpsT"
                     /locus_tag="PLL28_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNF69319.1"
                     /translation="MAHHKAAKKSIRQSEKRNEANRYYGKTTRNAIRKLRATTDSKEA
                     DKMLPEVVSMIDKLAKRNVIHKNKAANLKSSLMKKAVAK"
     gene            7315..8010
                     /gene="radC"
                     /locus_tag="PLL28_08070"
     CDS             7315..8010
                     /gene="radC"
                     /locus_tag="PLL28_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNF69320.1"
                     /translation="METSTNYTLKHLAEEDRPREKMLAFGRQSLTVAELIAILIGSGN
                     TGETAVELSTRILAHYGNDLNAIGKLQVSDLTQFNGIGEAKAVSIIAAMELGRRHALA
                     VAELKTQVRSSNDIFENIHPHLSDLAHEEFWVIYLNKANRIISKEKVSSGGVAGTVVD
                     VKIILRLALQKLASGIILAHNHPSGNLQPSDADISVTKKLRDAAKLMEIAVLDHLIIG
                     DRDYYSFADNGIL"
     gene            8020..8931
                     /locus_tag="PLL28_08075"
     CDS             8020..8931
                     /locus_tag="PLL28_08075"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNF69321.1"
                     /translation="MDPAYRKAYWAYHLCVFLWGFTAVFGRIIDLNETILVWYRMGIT
                     SAILLCIPSLWKTIRQVDKKTLLRLIGIGVLVTIHWISFYGSIKSSNISVALTCLATT
                     SFFVSVFEPLLFGKKFVWHELLLALLVIPAMYCIFHFSGNYKTGIILGLISTVFATLF
                     SLLNKKMTKVTAPLNITFIEIFTGFILLSAIMPLYLHWQPEADLQPSTMDGINLILFA
                     GICTVVPFTLSLYSMRHISVFTASITLNLEPVYGIIMAIVFFHENDHLEPGFYIGTLL
                     ILLVVFINPFIQRKFGGNVQAPLTIGE"
     gene            8979..11213
                     /locus_tag="PLL28_08080"
     CDS             8979..11213
                     /locus_tag="PLL28_08080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNF69322.1"
                     /translation="MPKQQTHNTKAVRFPTDFELFFRISADVMMVLDIDGKVIDFNNA
                     YERISGYTREELFALNGGWDLILQDDLPKLYDLLSTFSKDRSEVLNFTLRKRMKNGTI
                     KTISYDAILNKITNHIYAIGRDITNIMQQEQRMEDTQSRLQFFFDHALDGMSIIVDGK
                     YQMVNNAFMRIFGCSSPEDVVGRNFLDFVDSEFKEKVGRIVETNSDELYTSRIIRIDS
                     GEARNVEVTGKTIQHHNQKVRISVVRDLDDRRNLQALVKAGEERFKTVFNNAALGIVL
                     TDFAGNIVESNYTFMQRHGYTWDEIRNTQFCELFHEEDKPGVVRYMEALRNGQIENAF
                     VEKRLITKNGVVAWSKMTCSTMVGAHGETLLLSLLENIDSQKRSEQALIESEVKFRAI
                     YENSPMGIVITAAPGVIIDANPAFTKMMGYSDTELFNLPIIDLVHPADAISSGNWLQK
                     IYKNEIPHYSIEKRFDRKDGTSFWAREVMSNMFSIGSISLSVAIIENIETQKRTEEAL
                     EQKNIELTNTNQELEHFAYVASHDLQEPLRTITSFIQILDKRYRNSLDEDAGQFIGFI
                     VDGAKRMQTLIHDLLEYSRVNRFNTQFEKVDLNDVFHGVSSALKDKIENNDALVMAEQ
                     LPIVTGNRIQLTQVFQNLIDNAIKFKAKRRKPEVVVSVIEHANNWELIFADNGIGISQ
                     EYFQRIFIIFQRLHTLEEYTGTGIGLAICKKIIERHGGEIWVNSKPGKGTSFHFTLSK
                     HVVH"
     gene            complement(11226..12227)
                     /locus_tag="PLL28_08085"
     CDS             complement(11226..12227)
                     /locus_tag="PLL28_08085"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNF69323.1"
                     /translation="MKRVVAFWICCLCASVTYAQDIHFSQFFAAPILVNPANTGNFIG
                     SARIGLNYRDQWGSVTIPYRTFSAYADAGIQPKKAVNRFGIGLAALTDVAGDGSLTTN
                     KLYLSSAYHIGYTEHDAVRFAVGISGGMVQKTIDISKLYFDTQWDGFEFDTSTISNET
                     FSTTTINYPDINIGAMLTILPYEGRRIFVGASASHINKPNESFFGETNELGIKFTATA
                     GGSFATAGVANFQPQVYVSTQKSALEVIAGSNISIPMDVEGNINNAIFIGAWYRYGDA
                     AWLCGGFIADNFTASVSYDFNLSKLRTASSTMGGLEIAAAYVFGNTGKKDPLKCPAY"
     gene            complement(12224..>14345)
                     /locus_tag="PLL28_08090"
     CDS             complement(12224..>14345)
                     /locus_tag="PLL28_08090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNF69324.1"
                     /translation="IVFKFNPSLTTLAWSSFIGGNQEDGAYSMKINSLGQPVVCGGTA
                     SSDFPVTAGSWDNTYSGGVTDGWVAKIAADGSAILDATFAGTNNYDQVFFVETDEADN
                     IYITGQTKGTWTVTPGVYTEAGGKQFITKLESDLSAVIYSMVFGSGGSAINISPSAFL
                     VDDCENVYVSGWGGSVNQGYNFATGTTSGMTITPDAIQSTTDGDDFYFFIVSKNAIDL
                     LFASYFGGPSSPEHVDGGTSRFDKSGAIYQAVCAGCWGLDDFPTTAGAWSETNGSFLC
                     NLGVARIDLNVGGIYASAAADPSFIGCAPFDVSFINTSSDAIDYIWDFGDGSPVSNAF
                     EPSHTYLDPGTYDVMLIVIDSNSCNLADTAYLEVQVLADSLSAEFEYTSVENCDSLIA
                     TFTTIGFLLPTTTFLWDFGDGTTSTLINPSHTYFDPGSYTVTLIVTDPFSCNGVDTFS
                     LTFNYLFEFNTGFISEALGCLPVDATFTASFTGSDQYLWILGDGTIDSGEVVTHTYTV
                     AGTYEVSLIMLNCGIPDTVTQIVVIDGWPVAYFDDDPYYIIKNTTVTFTNLSTNAVTY
                     EWDFGDGGTSTEVNGKHIFTELGTYQVCLTATNSNGCSDVYCREVVAEAQGAIGVPNA
                     FSPNNDGVNDVLFVMGFGIADMQFLVFNRWGELVFSTDDYQVGWDGTYRNAQQEMEVY
                     VYVLSGTFADGNPFNLQGNITLIR"
BASE COUNT         4358 a         3030 c         2960 g         3997 t
ORIGIN      
        1 cagctgcatg acacagccct aatgcttgcg tcatggcccg gcctcacccc agccccacac
       61 ctcctttcgt cggagcggca agctctccac tccttcgtcg tagagaggga gcagcttttg
      121 tatttaatgg aaattattcc taaacattca cttccaaaat gaatgcgtaa ttagggctgt
      181 tgtcagcatc tgcaaatgcc ctgtaatggc gagagagatt ttagtttttc tgagggttat
      241 gtggaatgtg tttgatgtat tttagcatgt gctttttatc tgaccatatg atcatacacc
      301 cctaaacttt tggtgctacc tttttgtcaa gaaaaaggta gcggagagtg ttttacgaag
      361 aatgatcttg aagattactt tttacctttt taaatgcggc gaacttgaaa ttcaattttc
      421 agatgatgga cactttagta acatgaccac ttttagaaaa attgttaaac ttcaatagcc
      481 cgcataactc acaatgaatt tttgagcaat cacattcttg ccatcttcac ctgtaacaaa
      541 gtacattcct ttaggtagat ttctcaaaga gtaatttgac atccctgatt cggcttggaa
      601 ggtatataat ttggagccgg atatattgca tatggtcaag cgtattgatt ctgttgatac
      661 atttttaaca tatagcaaat tggcactgaa ccatacagaa aaagtgctat ctgaactcga
      721 ttgattatac acattcattt gtgccaatgc aacagagtct accccggttg taacgcgtat
      781 cgcagatata aatgagtcgc tgatgctcca gtgatcagtg tagcctacaa gcaccgcttt
      841 ccctgagggt tgaaataata aggctttggc ataactatca taataattat tttcatacat
      901 atctgattct agatagccat cagagttaaa gtcatagtcc ctggtgccat ccttatggag
      961 tctcaagaag taataattat atgaatcact attgttctgt ttaccggtgc cacccccaat
     1021 gattatctta tcatccggtt gtatgagcac agaatatgct tcgtcgcgac aataattaaa
     1081 aaacatttca agttttccat cattgctaaa accatcatat aagctgccgt cgacattata
     1141 acatgctatg ctagttcgtg ttttataata acagttaccg gagcttggaa gtttggaata
     1201 accaacagct atgattttac catcgctgag aatgccaaga tcggtgatga aatcatcata
     1261 gccgatatcg gttgttacct caccccattc accaaatgta ttatccggta caccattcgg
     1321 taggtatcga atcaaagcaa aatcactatt cgttctgcca cccacaagaa ttttaccgtc
     1381 agtctgcact tgaattgtcg cggcgcttcc tgagatccaa tcgaaatagc aatagacaag
     1441 cccggctcct ccaaaggaac tatccggtgt gccatcaggc agacatcttc cagtgacaaa
     1501 ttgatctata aatccggtat tttccgaatg ttcaattcca caaaatacaa ttttgccatc
     1561 ctcctgaact gcaatatctt ttacctgggc atagttctga tcgtatgaag agaaaaattt
     1621 cgcaatgcca ctgttgccga aagtggtatc aagtccacca ttttgattac atctgtagag
     1681 aacgccataa gtatctccta aaaaagttac atatccacct gcaacgatct ttccatcaga
     1741 ttgaagagcg attgcattta cttgtgattc ctcaatttca ccatcatcgg aaagcacctc
     1801 gcagcctgtt tcattaaata acacatcagg tgttgcgtct ggattcaggc gcgcaattaa
     1861 tgctttccga actcctcctt gaatcgcact gccggcaatg gttataaaac catctgcatc
     1921 tacaacagcg tcggcgcata attcgtctgt tactgggagg ttagttttaa cgagccctcc
     1981 ttcgccaaag ctatcgtcta attcaccgtc ttgagcaaac acaaaacggg taaaaaaaag
     2041 taacatcatt aatatcgtga gccgcaatgc cctggtatta attctaatat cctgcatgac
     2101 ctacaaaaat tttagttgcc ggcatttcat cagctgttgt gcctttaata atatataagc
     2161 cttttggtaa atcactaaga tctataatcg taatgggaga tgtgatttca taggtgaaaa
     2221 ccttttcccc ggccatatta tacagattca acatataggg aagatttatg tcgctgatta
     2281 catataattc attatcatag atatacgaat aaggtaaagg aatattactt tcatcactaa
     2341 gaaaactaaa tgtttcagat ggcaaggaga ttgctatttt ctgtggggca ctcgactcga
     2401 aaagtccaat catcactgca tatcgcttat ctaatgcgat tgtgtgtcca tttatgtcaa
     2461 gtagttctgc tgataacaca gtatccaaat aaggctcagg atcaccaatt tttgtatagc
     2521 tgatccatgg aacgtgctct gctatctcag gtgtgaaatt tggaatatcc tcctctggga
     2581 caatatatat tctgtattgc ccgactccac tcattttagt tgggccattg aattggacac
     2641 gtatatctgc aggtgtcttt gtgaaatcta cgagtgagat cacaggggta tgatcgtact
     2701 gctcgcagta tagttctact tcaattccta tggtactgcc gtaataagtt gatgtatatc
     2761 ttgatgactc aaaactagca actgaagcgt gaatactatc acctaccgtc attatctcag
     2821 tataataatt ctgaaataat attggcaccg tatcttcaaa aatcaatgtg ttcacatccg
     2881 gcgtaacagt atatgtcagg ctgccatcag ggactacgct tacataatac gaagggtcat
     2941 tttctgcaat gatatcatga tcatcattca agatatatac gcgatattct gatatatatg
     3001 attcgtcacc tgccttatga aagctgacct gaaaatcggt gatatcgcaa ttactaccgg
     3061 gtttatcaat cgacaaacca tatggagaag gcacggcagt tagcttggtg tagatagtat
     3121 tacttggcag tgaaacatta ttttgcgaca ccatcacccc atcggccata gatacaataa
     3181 gtgctcggta ataatgcgga taatttccaa gcaccggcgc cccgttgatg tctgtcattg
     3241 tggccgggag cacaacatca tatgtcgggg ttccaatagc ttccacttcc atatatcgct
     3301 caggaggcag tgtttctaaa aatgcaagcg aaggaggtgt aggatgtgca tacgtatacg
     3361 gacataaaaa aatcctataa gcactcactg ttgattcata tgcagcatta tcaaactgta
     3421 cgcgaaaatc ggaagcgttt tcattctcat atacatcgga aataaaaaca tgctcagcaa
     3481 ccgatgcggt agggttggaa attagcaatg gaacatctgc cgtagcttcg tatgcaaaat
     3541 cctgcaccgc caaactataa ctcttaacac cctccacact tccattacta gaaataccca
     3601 atctcaccca accgtaatgc gtatcactcc caatcaaaaa ttttacaccg atgtatttat
     3661 ttaagccttt ccaattatca tagttaaatg catagataaa agaatcataa cttgaaaagt
     3721 gaataagttt aatcaagtcc agcgtattcc aattagcagc actgttgata gaaaaccctt
     3781 ctatcaagtt catggctcca taagatgaag gataggatac attggaaatc actccgctga
     3841 aatttgatat ttgaacagaa aaatcacccc accatgccat cgtataatct gtccaaaaag
     3901 taagatcata atagtaatac tcggtgatgg tcaagtcgtt cataccatcg gcattgaaat
     3961 cgatatctac atgtccatca tttgagaata cagaatcggg gtcaatgttg tggtatatca
     4021 cctgcccatg cagtccggca ggcaatgcaa gaaaggctcc agcgtgagag gagtattgta
     4081 taagcttttt aagtttttca ttcatggggt gaatgtttta acaaatgtac caaatttcta
     4141 ccaattcaac cacagcaaaa ccgattgaac catcggtatg cagctaggta attgtgagga
     4201 tttgctgatg agttgatgtg ggccattaga ataaaagtta cccaccaaaa taccccccaa
     4261 aacaaccacg atcatctcta cacctccaca cttccacatg gagcgcagcg acatccagcc
     4321 tacgcgagac ttccatgcct ccccaccctt ttccttacct ttgaacaaac aacaatatca
     4381 tgtggcagga agaaaataat caattgcata agactttcat gtttcacaat ttttcggaag
     4441 cctttgcttt tatgacgcgt gtggccatgc tggcagagca acacaatcac cacccctatt
     4501 ggacaaatgt gtggaacaag gtagagatat tccttaacac gcatgatgca ggtaatatcg
     4561 ttacggaaaa agatcatgct ctggcgaagg ctatcgatgc tttgttgtaa ctttttctgt
     4621 taatttgcaa ctatgaaata tgatcaggcc gcgcaacatc gattgatcgc gttaacagaa
     4681 caattggtaa aaaacctctc cgacaaaaat acaaatgcgg atactgcgcg cgataccatt
     4741 gatgatctca aacaagtatt gcattttcat gactggaagt attatgtaga tgcatctccg
     4801 gtaatcagag atatcgatta tgacctatta ttcaagaaat tacaatcgct cgaaaaatca
     4861 tacccggaat tacttactga tgactcccct acccagcgcg ttgccagaac attgagtgat
     4921 gatttcccca cagtatcaca ttctgttcct atgctgagtt tggaaaacag ctatgatgag
     4981 aatgacctga atacgtttga taaacgcgtt cgcgaactga cgagtactga caatatcatt
     5041 tactgcgttg aaccaaaatt cgacggctcc tccattgcag tcatatacga aggtgatata
     5101 cttgtacgcg ctgcaacacg cggtgatggt attcagggtg atgagatcac catcaacgca
     5161 aaacgtatgc gctcattgcc gttgcgcgct gatttctcta aatacgggat tcataaagta
     5221 gagatacgcg gtgaggttgt aataggaaaa gaaacctttg cacgcatcaa tgaaaaacgc
     5281 gaaagcgaag gccagcaaat cctccaaaac ccacgaaact ctgccgcagg agcactgcgt
     5341 gtgaaagatt cggaagaaat agaacgccgc ggactggaag catttatgta ccatattgcg
     5401 tatgctgtcg acaaggatgg caacgatttg cttggcgatc aattgagata tcacttcgac
     5461 aatatcgaga tgctcggaaa actcggattt aaaattccgg ggaaggaaaa aaagaaatgc
     5521 cattcagttg aagaagtgca tgctttcgta caacaatggg aacaagatcg cgacgactat
     5581 acttacgaga tcgatggcat ggtgataaaa gtaaatgaca tcgcgcagca aaaaatatgc
     5641 ggtgccactt cgcatcatcc ccgctgggca attgcattta aattcaaagc acgcgaacag
     5701 gaaacagaat tattatctgt tgaatatcag gtaggaagaa caggcgcgat tacacctgtt
     5761 gctaaagtga aaactgttta tatcggtggt gtgaatgttt cttcaatttc attgcacaat
     5821 gaagacatca tcaaagaaaa agatttgcat ctgcatgata tcgttgtagt gcaaagggca
     5881 ggcgatgtaa ttccttatat cgatcgggtt gtgcttgcaa aacgagataa tagtaaaatc
     5941 aaaactattg atttcatcaa agaatgtcca tcgtgcaacc aagctattta caaacctgaa
     6001 gaagaagctg tttaccggtg tattaaccct gagtgtccgg cacaaatgga agaacgcctc
     6061 atccatttcg tgagcaagga tgccatggat atcgatggct gcggtcgaga aaccatcagc
     6121 gaattatatc aggcaggaaa gattaccacc atacccgata tttttgattt gaagacatcc
     6181 gatcttgcag gtcgcgaagg gtggaaagaa aaaagtatcg ccaaactcat cgatggtatt
     6241 gaggcttcga aaaagcaacc gttgtggcga ttggtgaacg ggcttggcat tcgtcacctc
     6301 ggcacacaaa cggcaaaaga cctggtaaag aatatcagcg atctgacaga gttgtttgac
     6361 tgggacctgg aacgatacca ggcaattgaa ggcattggtc caaaggtggc ggcgagcatt
     6421 catcaatatt tttcgaatgc cttcaaccaa cagatgattt tgaagctcaa agaagacggg
     6481 ttgaacatgg cgaacgagaa aaccgcagga ggcagtggca agctttcagg caagaccttc
     6541 ctgttcaccg gctccctgac ccgctttacc cgcgacgagg ccaaagaact ggtagaagcc
     6601 aatggcggca aactgatagg ctctgtctcc aagaacctga actacctggt agcaggcgaa
     6661 agcgcagggt ctaagctgga caaggctaag gctttaggga ctgtagagat cattgatgaa
     6721 gaggcttttt tggctatgat agcctgacag aagtcccggg tggggagaat tttggtcctg
     6781 caattgccat ttaaggccgt ttagaggctc tcagccgtgt ttggaggtca tttcgacaag
     6841 taaggagggc tcgaattggc gttatggcgt taaaaatccg tttgtggtaa gtaaaaccca
     6901 acattctatt tgtaattatc cacaatgctc cctatatttg cactccattt taaaaactaa
     6961 gaggaaaaac aatggcacat cataaggccg ctaagaagtc aatccgccag tcggaaaagc
     7021 gcaatgaagc gaatcgttac tatggcaaaa caacacgtaa tgccatccgt aaactccgtg
     7081 caactacaga ttctaaggaa gctgacaaaa tgttgcctga agtggtgtct atgattgata
     7141 agctggcgaa acgcaatgtg attcacaaga ataaagctgc caacctgaaa tcttcattaa
     7201 tgaagaaggc agttgcgaaa taattaattc aagcactatt tttttttgcc cggtttcatc
     7261 catgaagccg ggttttttta tttttggagc gagggatgtg atgtatgaac tgtgatggaa
     7321 acttcaacga attataccct taagcatctg gctgaggagg atcgtcctcg cgaaaagatg
     7381 ctcgctttcg gtcgccaaag ccttaccgtt gccgaattga ttgctattct tatcggttcc
     7441 gggaataccg gagaaacggc tgttgagcta tcgacacgca ttttagctca ttatggcaac
     7501 gatctgaatg ccatcggcaa attgcaggta agcgacttga cgcaattcaa cggaattggc
     7561 gaagcgaaag ccgtaagcat aattgccgca atggagctcg gtcgacgcca tgcacttgct
     7621 gttgccgagc taaaaacgca ggttcgatcg agcaacgata ttttcgaaaa cattcatcct
     7681 catctgagcg acctggctca tgaagaattt tgggtgatct atctgaacaa ggcaaaccgc
     7741 atcatcagta aggaaaaagt aagctccgga ggtgttgcgg gaactgtggt ggatgtgaaa
     7801 attattttgc ggctggcatt gcagaaactg gcgtcaggca tcatcttggc gcataaccac
     7861 ccgagtggaa atcttcaacc cagcgatgcc gatatttctg ttaccaaaaa gctacgcgat
     7921 gccgcaaaac tgatggagat tgccgttctc gaccatttga tcatcggcga ccgtgattat
     7981 tacagttttg ccgacaatgg aattctctaa ctttgtgcta tggatccggc ataccgcaag
     8041 gcctattggg cctatcatct ctgtgttttc ttgtggggtt ttacggcggt gttcggtcgc
     8101 attattgatt taaatgaaac aatattggtg tggtaccgaa tgggtatcac ttcagccata
     8161 ttactttgta ttccttcact ttggaaaact attcggcaag tagataaaaa aacgctgctg
     8221 cgattaattg gtatcggcgt gttagttacc attcactgga tatcatttta cggatctatt
     8281 aaatcgtcga atatttctgt cgcactgact tgtttggcaa ccacatcctt ttttgtatct
     8341 gttttcgaac cgctgttatt cggcaaaaaa tttgtgtggc atgaattatt gcttgcattg
     8401 ctggtgatac cggccatgta ttgtattttt catttcagcg gaaattacaa aacggggatt
     8461 attctggggt taataagcac tgtatttgca accttgtttt ccttgctgaa caagaaaatg
     8521 acgaaggtta ctgcaccatt aaacattacg ttcatcgaaa tattcacagg tttcattttg
     8581 ctgagcgcca ttatgccgct ttacctgcat tggcaaccgg aagcagattt acaaccatcg
     8641 accatggatg gcatcaatct gattttattt gccggtattt gcacggtagt gccatttact
     8701 ctttccctat acagcatgcg tcatatttca gtatttacgg cgtctatcac cttaaatctg
     8761 gaacctgttt acggtattat tatggcgata gttttctttc atgaaaatga tcacctcgaa
     8821 cccggatttt acatcggcac cctcctgatc ctgcttgttg tatttatcaa tccatttatt
     8881 caaaggaagt tcgggggtaa tgtacaagcg cctttgacca ttggcgagta agcattcggc
     8941 gaatttcatg tatttttagg gagcaatttt atcctgcatt gccaaaacag caaacacata
     9001 acaccaaagc tgttcgattt cctaccgact tcgaattatt tttccgcata tccgctgatg
     9061 tgatgatggt attggatatt gacgggaaag taattgattt caataatgct tacgagcgca
     9121 tttcaggata taccagagag gaattgtttg cactcaatgg cggctgggat cttatactcc
     9181 aagatgatct tccgaaattg tatgatttgt tgagtacatt ttcaaaggat agaagtgagg
     9241 tgctaaattt cactttgcgc aagcgtatga aaaatggcac tattaaaact atttcgtatg
     9301 atgccatttt aaataaaatc accaatcata tttatgctat tggtcgcgac atcaccaata
     9361 tcatgcaaca ggagcaaaga atggaggata cgcaatcgcg ccttcagttt ttctttgatc
     9421 atgcgttgga tggcatgagt attattgttg acggcaaata tcagatggtg aataatgctt
     9481 ttatgcgcat tttcggatgc agctcgccgg aggatgttgt tggcagaaat tttcttgatt
     9541 ttgttgattc cgagtttaaa gaaaaggttg gacgcattgt agaaaccaac tccgacgaat
     9601 tatatacttc ccgcatcatt cgcatagata gcggtgaagc aagaaacgtt gaggtgaccg
     9661 gaaaaacaat tcaacatcac aatcagaaag tacgcatttc agtagtgcgt gatcttgacg
     9721 accgtcggaa tttacaagca ttggtgaaag ccggagagga gcgatttaaa actgtattta
     9781 acaacgccgc tttagggata gtgctcactg attttgcagg aaatattgtt gaatcgaatt
     9841 acaccttcat gcaaaggcat ggttatacat gggatgagat cagaaataca caattctgtg
     9901 aattatttca tgaagaggat aagccgggag tagtgcggta tatggaagca ttgcgcaatg
     9961 gtcagattga aaatgcattc gttgaaaagc gattaattac aaaaaacggc gttgtagctt
    10021 ggtcgaaaat gacctgcagt acaatggtag gtgcacatgg cgaaacacta ttattatcat
    10081 tactggaaaa tatcgatagt cagaaacgca gtgagcaggc attgatagaa agcgaggtga
    10141 aattccgcgc catatatgag aactccccaa tgggtattgt aattacggca gcacccggtg
    10201 ttattataga tgccaatccg gcatttacca aaatgatggg ttatagtgat acggaattat
    10261 ttaatcttcc aattatcgac ttagtgcatc ctgcagatgc cattagctca ggtaactggc
    10321 tccaaaaaat atataaaaat gaaatcccgc attactccat agaaaagcgc tttgaccgca
    10381 aggacggcac ttcattctgg gcacgggagg tgatgtcgaa tatgttcagc ataggcagca
    10441 ttagtttatc tgttgctatc atagaaaaca ttgaaactca aaagcgcact gaagaagcgc
    10501 tcgagcaaaa gaatattgaa cttaccaata ccaatcagga attagagcat tttgcttatg
    10561 tagcgtctca cgacttacaa gagcctttgc gtaccattac cagtttcatt caaattttag
    10621 acaaacgtta tcgcaatagt ttggatgaag atgccggtca atttatcgga tttattgttg
    10681 atggtgccaa gcgcatgcaa acactcatac atgacctgtt agaatacagc agggtaaatc
    10741 gcttcaacac acagtttgaa aaagtggatc tcaacgatgt ttttcatggt gtcagttcag
    10801 cattgaaaga caaaattgaa aataacgacg cattagtgat ggcagaacag ttgccaatag
    10861 taaccggaaa tcggattcag cttacacagg tatttcaaaa cctgatcgac aatgccatca
    10921 aattcaaagc caagcggcgc aaaccggaag ttgttgtaag cgtaatcgaa catgccaata
    10981 attgggaatt aatttttgcc gacaatggga ttggcatcag tcaggaatat tttcagcgca
    11041 tcttcatcat cttccagcgt ttgcatacat tagaagaata taccggaaca ggaataggtc
    11101 ttgccatctg caaaaaaatt atcgagcgtc atggcggaga gatttgggta aattcaaaac
    11161 cgggaaaggg aacaagcttt cactttacac tttcgaaaca tgtggtgcat taataaaaat
    11221 gttgcttaat aagcaggaca tttcaatgga tctttttttc cggtattgcc gaacacataa
    11281 gctgctgcga tttccaaccc acccatagtg ctgctggcag tgcgcagttt cgacaagtta
    11341 aaatcgtagc ttaccgatgc cgtaaaattg tcggcaataa atccgccgca tagccatgct
    11401 gcatcgccat agcggtacca cgcaccaatg aatatggcgt tattaatatt gccttcaaca
    11461 tccataggta tactgatatt acttcccgca atgacttcaa gtgcactctt ttgggtgctc
    11521 acatatacct gtggttgaaa attggctaca ccagcagtag cgaatgaacc acctgccgta
    11581 gccgtgaact tgatacccaa ttcattggtt tcgccaaaaa aagattcatt tggtttatta
    11641 atatgcgatg cagaggcgcc aacaaaaatg cgtcgacctt catatggtaa aattgtcagc
    11701 atcgcaccga tattaatatc cgggtaattt atagtagttg tagaaaatgt ttcattggaa
    11761 attgtactgg tatcaaattc aaagccatcc cactgcgtgt caaaatacaa tttcgatata
    11821 tcaatagttt tttgcaccat gcctcctgaa attccgacag caaagcgaac agcgtcgtgt
    11881 tcggtgtagc cgatatgata tgctgaagac agatataatt tatttgtagt aagtgatcca
    11941 tcgccggcta catcggtaag tgcagccaag ccgattccaa agcgattaac tgccttttta
    12001 ggttgaatac ctgcatctgc atatgcggaa aaagtgcggt atggaatggt aacactaccc
    12061 cactgatcgc gataatttaa tccgatacgt gcagatccga taaaatttcc tgtgtttgca
    12121 ggatttacaa gaattggtgc ggcaaaaaat tgagaaaaat gtatatcctg cgcataggtg
    12181 acacttgcac acaaacaaca gatccaaaat gccactacac gtttcatctg atcaatgtta
    12241 tatttccttg tagattaaat ggatttccat ctgcaaatgt gccgctaagc acatacacgt
    12301 atacttccat ttcttgttgc gcatttcgat atgtgccgtc ccatccgacc tgataatcgt
    12361 ctgttgaaaa tactaattca ccccatctgt taaatactaa aaattgcata tcggcaattc
    12421 cgaaccccat aacaaacaat acatcattga cgccatcgtt attcggtgaa aatgcgtttg
    12481 gaacacctat ggcgccttgt gcttcagcga caacttctct gcaataaaca tcagaacatc
    12541 catttgaatt agttgctgtg aggcatacct gataagtacc caactcggta aaaatatgct
    12601 tgccgttcac ttcggttgaa gttcctccat ctccaaaatc ccattcgtaa gttacggcgt
    12661 tggtgctgag attagtgaat gtgacagttg tgttttttat aatgtaatac gggtcgtcat
    12721 cgaaatatgc aacgggccaa ccatcaatta caactatttg tgttacggta tccggaatgc
    12781 cgcaattaag catgatcaga gaaacttcat aagtaccggc aacggtataa gtatgtgtaa
    12841 caacctcccc tgaatcgatg gtgccatcac ccaatatcca taaatattga tcgctgccgg
    12901 taaagcttgc cgtaaatgtt gcatcaacgg ggaggcaacc taatgcttcg cttataaatc
    12961 cggtattaaa ttcaaataaa taattaaacg tgagactaaa tgtatctaca ccattacaac
    13021 taaaaggatc tgtaacaatc aaggtgacag tataagaacc cgggtcgaaa taggtatgcg
    13081 atggattgat gagtgttgat gtggttccat caccaaaatc ccacaggaaa gtagttgtag
    13141 gcaacaaaaa tcctattgtt gtaaaagttg caatcaggct atcgcaattt tcaacggaag
    13201 tatattcaaa ttccgccgac aatgaatcgg ccagcacttg tacttcgaga tatgcggtat
    13261 ctgcgagatt acacgaattc gaatcgataa cgatcaacat tacatcgtat gtaccggggt
    13321 cgaggtaagt gtgacttggc tcgaaagcat ttgacaccgg tgaaccgtcg ccgaaatccc
    13381 agatataatc tatagcatca gaactcgtat tgatgaatga aacatcaaaa ggagcacaac
    13441 caataaaaga tgggtcagca gctgcgctcg catatatacc tccaacattt aaatcaattc
    13501 tggcaacacc caggttacat aaaaagcttc cgttcgtttc gctccatgca cctgcagttg
    13561 ttggaaagtc atctaaaccc cagcaaccgg cgcaaactgc ctgataaatt gcaccggatt
    13621 tatcgaatct gcttgtgcca ccatctacgt gctcagggct agatggtccg ccaaaatagg
    13681 atgcaaataa tagatcgatg gcattttttg aaacgataaa gaaataaaaa tcatcgccat
    13741 ctgttgttga ctgaatagca tcaggggtta ttgtcattcc tgacgtggta cctgttgcaa
    13801 aattataccc ttgattcaca gaaccacccc aacctgaaac atatacattc tcacaatcat
    13861 caaccaaaaa tgcggaagga gaaatattaa ttgctgagcc tcctgaacca aaaaccatag
    13921 aataaattac cgcacttaaa tctgactcca gtttggtgat gaattgtttt ccacctgctt
    13981 cggtatacac gcccggcgtt acagtccagg taccttttgt ttgaccggtg atataaatat
    14041 tatccgcttc atctgtttca acaaaaaaca cctgatcata attattggtg ccggcaaatg
    14101 tagcatcgag aatggcgctg ccatctgcag caattttcgc aacccaacca tctgtaacac
    14161 cacctgagta tgtgttatcc cacgaacctg cagtaaccgg gaaatcacta cttgcggttc
    14221 cgccgcatac caccggttga ccaagactat taattttcat tgaataagcc ccatcttcct
    14281 ggtttccgcc gataaacgaa ctccatgcaa gggtggttag cgatggatta aatttaaata
    14341 ctata
//
LOCUS       DAOQHY010000081        14321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2029_length_14321_cov_4.613487, whole genome shotgun sequence.
ACCESSION   DAOQHY010000081 DAOQHY010000000
VERSION     DAOQHY010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14321
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2029_length_14321_cov_4.613487"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PLL28_08095"
     CDS             complement(<1..280)
                     /locus_tag="PLL28_08095"
                     /inference="COORDINATES: protein motif:HMM:NF037744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69325.1"
                     /translation="MYQVTQHIVKLLYEHNCVIVPGLGGFVTNYQPAKVHPTSFIFNS
                     PGKSVAFNVSLSTNDGLLADTIARQEGISHRDADKLIVDFVSQIKTALS"
     gene            complement(351..1961)
                     /locus_tag="PLL28_08100"
     CDS             complement(351..1961)
                     /locus_tag="PLL28_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69326.1"
                     /translation="MKKFAIYLAFTGLISSQAIAQIVGDEDVDVVSGYNPVLADAVKE
                     TFEASLPKQDIKPDPQTYNIPIEFFQIPYQPVKVKPIRMPDVKPEELDNVFVKAGFGT
                     QLTPLAEAYLNSNRSKKYNYGLYGKYISSNSKIDFQNYSDTRVGGTAKFFFDDKYVLP
                     VNAYFSTNSLNYYGFDHEVDTTYTDADLKQRFTNYGFDLGFANTGDNPLNMDFGLKAG
                     LKGISDINKYKEINPFLNAWGEKKLENNSIAGASASYEYYQYTGFGDYYNSLVGIKPY
                     YKMITDQYSLTVGLDSRMDKDQNAYILPDAVFSYDLIGDKFVFVAGWQGRTQVNSFAN
                     IVTENPFVADSLIFKISEIHEKFAGLRGSTSGNFSFSVKGYQKSAQDLPFYVNDTTDM
                     KRFLVKYSDATIWGGALEMSYFDSDRFRVTGSVNAFKFSEVADLDQPWHRPTLEWTLS
                     GMYRFNKRMSMTADVFGVNKTLALLPGDITEEVKGCVDMNISATYVYSKYFNIFVNLN
                     NLANYKYQRYYNYPSYGIQAMAGLSFAF"
     gene            complement(1982..5053)
                     /locus_tag="PLL28_08105"
     CDS             complement(1982..5053)
                     /locus_tag="PLL28_08105"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF69327.1"
                     /translation="MCIVLCGTTGALKAQQSLIYTDLYANYKKAMELYENKMYATAQE
                     YFEQVGSVTPEGTDGDIFTYVSNSRYYAAICAIELNNTDAEKMMLDFVHNNPGNVMLG
                     SAYNQLGKLYYNKKEYTNAATWFEKVDVKDLSLEERDTYKFQYGYSLFNKKRFTEAKV
                     MFASLKDKPQNQYYNAANYYYGFIQQEDGNYENALTCFNIVKDVPPYNAVVPYYITNI
                     YFQQGKYEKVISYAEPLMADTKLVYFAEINSLIGQAYFYRNNYRKALPYLNYYIEKAK
                     NIRDEDYYQLAFAQYSTGDSKAALTTLKETSDDNDTLYQHIQYLYGNCYIALDKKEEA
                     RNAFMEASRIGEDKTIHEQALFNYGKLSYESGYHTAAVTAMQDYLKAYPKGSNKTEAQ
                     ELLANALLGTNDYLAAMDVIENIPQKTPKLKEAYQKVSYYSGVQLFNQKNFDEADKMF
                     MQSLENPIDVNLQAACYFWIGEIRFAENKYALTITNHVKFQDLAKVAKDLPSETRVPY
                     SDYTIGYAYFKQSNYSNAVNYFSKVTTALKTSKTDMVQNNVYIDASLRLGDCYFMTKD
                     YKKAETNYNKIIDNNAKGVDYAMYQKAMLQGLQNNSNGKIATLQSLTNKYPNSLYVDD
                     AMYEIANTYFITKKNTESIAAFKELVSEKPNSSYTIKAYLKLGLIYFNEKDYKNSEKY
                     YRLAYESSPNSEEGQQAKEGLADIVKETGNTGAADGVVEVTKIDEEKYNYAKNKYDLN
                     EFSGAAIAFSDYLREFPKGGYKSEAEFYRGESYYQLKQYAKALPDFESIIKGNKTKFL
                     ESALVRAGWIEYYQNEDYQTAYNYYERLYEVADFKENTFVAMVGLLQTAYFLNDFDAV
                     IVNANRILASDLVTNKERIDAHYYSGQAHLANNNIDKAYDAFSKAAALTTNEIGVESR
                     FHMADILFQKGKLEESKTKCNEIINNMPSYDEWIIRSYILLADIAAAKGDYAQAKATL
                     NSIISGYKGDPELVELAKQKLEQIEEMEKSGRRKSPASDTNDESEINFDNN"
     gene            complement(5258..5728)
                     /locus_tag="PLL28_08110"
     CDS             complement(5258..5728)
                     /locus_tag="PLL28_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69328.1"
                     /translation="MRLRLIFPILLLAIVSCNEPERGTLDPETMSKKLFDAMKSNSPE
                     KIALLLPDKGTFRKIEEENGHILVNLEAGYNGFEKQSSACLDTVRNKVSNWSSATFTR
                     GNYTESKLGKLPKARVTTKFDADGSPRKVEFTAVKFNNRWYYYGDMTWIDKIVE"
     gene            5833..6306
                     /gene="rnhA"
                     /locus_tag="PLL28_08115"
     CDS             5833..6306
                     /gene="rnhA"
                     /locus_tag="PLL28_08115"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HNF69329.1"
                     /translation="MVQIYTDGSARGNPGPGGFGTILRYGKHEKELSGGYRRTTNNRM
                     ELMAVIAGLEALTKEGLVITIYTDSKYVCEAIEKGWIYGWMKKGFAGKKNADLWTRLM
                     RAYARHKVKLIWIKGHAGHPENERCDVLATTAADGHKLAIDEVYEMENQTKLGFD"
     gene            complement(6350..7363)
                     /gene="fbp"
                     /locus_tag="PLL28_08120"
     CDS             complement(6350..7363)
                     /gene="fbp"
                     /locus_tag="PLL28_08120"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HNF69330.1"
                     /translation="MKVKTLIEFIVERQQDFPFATGELTRLLSDISIAGKIVNREVNK
                     AGLVDILGAEGSENMSGETQQKLDVYANNQFMNALMAGGEVCGFASEEEDNFISFDGA
                     ISKSGKYVVLIDPLDGSGNIDVNVSIGTIFSIYRRLSLSGGATLEDFLQKGTEQVAAG
                     YIIYGSSTMMVYTTGFGVNGFTLDPSIGEFCLSHPDIRIPNRGIIYSINEGNYVHFPE
                     GVKRYIKYTQEEDLKSQRPYTSRYIGSLVADFHRNMLKGGVYLYPETAKSPKGKLRLL
                     YECNPLAFIAEQAGGVATNGRKRILDIEPKELHQKTPFYIGSSAMVEKIVEFLDRFTE
                     AKV"
     gene            7444..7896
                     /locus_tag="PLL28_08125"
     CDS             7444..7896
                     /locus_tag="PLL28_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF69331.1"
                     /translation="MEYHIRPADGADIAMIHRLVSQLAEYERAADQVITTTADYQRDF
                     EAGCFQALVAVDLQSGEIVGMALYYFAYSTWKGRYLWLEDFIVTEQCRGKGVGRLLFD
                     AVGAIAAKENTFMKWQVLDWNTPAINFYNKYPMEVQDEWLTCRMPVRS"
     gene            complement(7924..8286)
                     /locus_tag="PLL28_08130"
     CDS             complement(7924..8286)
                     /locus_tag="PLL28_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69332.1"
                     /translation="MRYHSIVQLACWMMCHLWFIVFYTRPNIHPDWWEIMIFGSIVQI
                     MGIIIYAINKEFRFAWMILLAMPVIWIAGMYTDNADLDLSDLGEVFILWMFRIIIIHV
                     LYFLTIIPILRDVLYETE"
     gene            complement(8620..9009)
                     /locus_tag="PLL28_08135"
     CDS             complement(8620..9009)
                     /locus_tag="PLL28_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69333.1"
                     /translation="MRHFFSNNASRAAIVFLPVILFLIACDPGSCITFKIANESNGVV
                     DVTYFEYDSNKVTQIRIPKRTEQILFQQCFLGYVDRFENQDSVDLSYHVRIWKDSVLL
                     DTNLNDKQSWDYIRMNNSEANLTLRIK"
     gene            complement(9032..9397)
                     /locus_tag="PLL28_08140"
     CDS             complement(9032..9397)
                     /locus_tag="PLL28_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HNF69334.1"
                     /translation="MATTKSHEARIAAMTFASVYPLYLAKIEKKGRTKDELHQVIEWL
                     TGYNLVTLQKLIEKRVNFADFFDKAKLNPNAKLITGMICGYRIEEIQDPLTRKVRYLD
                     KLVDELAKGRAMEKILRKA"
     gene            complement(10036..10758)
                     /locus_tag="PLL28_08145"
     CDS             complement(10036..10758)
                     /locus_tag="PLL28_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69335.1"
                     /translation="MPHFKPTPNSFPQVRKNLLLKTIPVSLIAGVGGFLIGFYKADWD
                     EIAGVYLPLILLFVIGVNLLVTYYVANKRRAIFESYVLTINEEDITREQLNTPSITIP
                     RIEVRDIIKNLSGDIIIRGRTKLDVIHVPAQLEDMANLEVLLAEVHPIAVGFNASRIQ
                     LYRRGLSLLSIGLMAVVYLSENRLFVAISGIALMTLMGYSYHQYYRSKNVDHASKNLV
                     WWALVAMVSIGFIVCMKVLEIK"
     gene            complement(10811..11038)
                     /locus_tag="PLL28_08150"
     CDS             complement(10811..11038)
                     /locus_tag="PLL28_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69336.1"
                     /translation="MKTIEHKFIHELIKLEKEQTFSGRSIIGRNKLLVVGTFNPEDNM
                     VCKQNEAEWFYGRNVNNLWKYIPEAIVVASS"
     gene            complement(11185..12111)
                     /locus_tag="PLL28_08155"
     CDS             complement(11185..12111)
                     /locus_tag="PLL28_08155"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69337.1"
                     /translation="MKGKFLLEQYAGFAAGFLLLHQQAQTQVVYTDIEPDLVFQDDGD
                     FQYIDMNDDGIYEFGFLKSTNIISTLSSPGLNTTWYYRVAIWVGPAVVGPQIAGESVH
                     NSSAGGTYYFPYALNQGALINNDLEFQAGGFQLMASKTAEIYYGYSFTILGVGDWHLQ
                     PANWNNLLLDSIRYLGVRFEDQENCLHYGWIRCVVMDSVDKLIVKDFAYETQCNQPIL
                     TGDTISYVDIASTNSSPDFSIYTFEDNIYIHKISNDNFDVIIINIAGQAVFHETIIEN
                     TNAIDMRNQPKGVYLVTIKNNERSYSKKVVIE"
     gene            complement(12114..>14321)
                     /locus_tag="PLL28_08160"
     CDS             complement(12114..>14321)
                     /locus_tag="PLL28_08160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69338.1"
                     /translation="ITNETDDPDDIIDNIQADEPAYLSSIKTDLTSDGDCDYCHEWMD
                     AVKYQKDLEGYYGKWATQDEIPFPVTDPRHEYINQFIDLVNEIETAHGYKIDVILDLN
                     ILSESAAQCKAIVKYMRDHTKNGVTDVNVVGVEIGNECYLNWGADLMGFDVFGDYWNY
                     INGGNSALTADERNYIFGDLPVKTSHNYINQFKGDIDFNCKVGIPADNPQNTETITYM
                     FREGAEKGYPITTGWNSSLANPVIYGSTITAGTETRYKFDAVILHTYYDATHNYKQIP
                     LDNLCLFYPSDGFPSCNLAEGCPTGSNWQYGTYDSRLVAAYSGILGTTLAPPFGNFKT
                     ALKTRYLEYYDKQKAVLKFSPSNPYPKELWNTEWNLKDKVNSNEISDEEQDLISIYNN
                     SFPHGLITQELVLKNIKLNYTLGYIPSFFTYSTFHNFAGGGWTDMLCIADCADCKNHI
                     NDDGSPNPLNPALYTGDVNYYMRRTEYYVMELLSEITKNNLQYLPANFSMYFTNPNLQ
                     PTVFIDPEHENLYVYFTNMKAETQSYAVNPNHLMDLYPTAVSLGFEDATIYTIQANQP
                     YSNSGLSTLFKIHTCYSCHNLFDGLDNPHPFEIQRISDPIPNDPECSDGLPAGAMCIT
                     VPKYSIGYFKIPIHVNYDPQQKTGVEDLSQFVTLYPNPTSGVFQIRSEISGLFSDAQL
                     RVTVYSVTDKVVFETKAAENQPIDLSHLPSGLYLVAIKDETDLTISKQLIKIE"
BASE COUNT         4109 a         2900 c         2854 g         4458 t
ORIGIN      
        1 ctgaaagcgc tgtttttatc tgtgatacga aatcaactat cagtttatcg gcatcgcgat
       61 gtgaaattcc ttcctgacgg gcaatagtat cggctagcag tccgtcgttg gttgagaggc
      121 tcacattgaa agcaacagat ttgcccggac tattgaaaat aaatgaggta ggatgaactt
      181 tcgcaggttg gtagttggta acaaagccgc caagaccggg cacaatcacg caattgtgtt
      241 cgtacagtag tttgacgata tgttgtgtta cctgatacac tatccccgtg tttcaggcaa
      301 atttcggctg ctaacacaca attaacaccc taaagtaatc caccgactgt tcaaaacgca
      361 aagctcaacc ctgccatggc ctgaatgcca taggaaggat agttgtaata gcgctgatac
      421 ttgtaattgg cgagattgtt caggttgacg aatatgttga aatacttaga atagacgtaa
      481 gtagctgaaa tattcatatc aacacagccc ttaacttctt cggttatgtc gccgggaagc
      541 aaagccagcg ttttattcac cccaaataca tcggcagtca tagacatgcg tttgttaaag
      601 cggtacattc ccgaaagtgt ccattccaac gttgggcgat gccaaggctg atcgaggtct
      661 gcaacttcag aaaacttaaa ggcattcact gaacctgtca cacggaaacg atctgaatca
      721 aagtagctca tttcgagggc accaccccaa attgttgcgt cgctgtattt cacgaggaaa
      781 cgcttcatat ctgtagtatc atttacatag aatgggaggt cttgggcaga tttctgatat
      841 cctttaaccg agaaactgaa atttccgctg gttgaacccc tcagcccggc aaatttttca
      901 tggatttcag agattttgaa aatgagtgaa tccgcaacaa atggattttc tgtaacaata
      961 ttagcaaagc tgttcacctg agtccttccc tgccagcctg ctacgaaaac gaacttatcg
     1021 ccaattaagt cataactaaa taccgcatcc ggtaaaatgt aggcattttg atctttatcc
     1081 attcgggaat caagtccaac ggtaagtgaa tattgatcgg taatcatttt gtaataaggt
     1141 ttgataccta ccagtgaatt ataataatca ccaaaaccgg tgtattgata atattcatac
     1201 gatgcggaag caccggcaat actattattc tccaatttct tttcacccca tgcattcagg
     1261 aaggggttaa tttctttgta tttattgata tcactgatgc ctttaagtcc ggctttgagt
     1321 ccgaaatcca tattcagggg attatcgcct gtattagcga aacctaaatc aaaaccataa
     1381 ttggtaaatc tttgtttcaa atcagcatcg gtataggtag tgtccacctc atgatcgaaa
     1441 ccataataat tcaaagaatt tgttgagaaa taggcattga caggcagcac atatttatcg
     1501 tcgaagaaga attttgctgt acctcctacc ctggtatctg aataattttg aaaatcgatc
     1561 ttactattcg acgaaatata ttttccatac aacccgtaat tatacttctt gctgcggttt
     1621 gaatttaaat aggcttcggc caaaggtgta agttgcgtac cgaatccggc tttaacaaat
     1681 acattgtcca attcttccgg tttcacatcg ggcatacgga taggtttaac cttcaccggc
     1741 tgataaggta tctggaaaaa ttcaatcggg atattgtacg tttgcggatc cggtttaata
     1801 tcctgcttag gcaagcttgc ttcaaatgtt tctttgacag catcagcaag tacaggattg
     1861 taaccactca ctacatctac atcttcatct ccaacgatct gagctattgc ctgcgagcta
     1921 attagcccgg taaaggccag atatattgcg aatttcttca tagtctttgt catgttaatc
     1981 atcaattatt gtcgaaattg atttctgatt catcattggt atctgaagcc ggacttttac
     2041 gtcgacctga tttttccatt tcttcgattt gctcgagttt ttgtttagca agctcaacca
     2101 attccggatc acctttatag ccactgataa tactattcaa agtagcctta gcctgcgcat
     2161 aatcgccttt tgcagctgcg atgtctgcta ataaaatata gctcctgatt atccactcat
     2221 cgtacgaagg catattgttg atgatctcat tacatttagt tttagattcc tcaagttttc
     2281 ctttttggaa taagatatca gccatatgaa agcgtgattc aacaccaatt tcattcgtag
     2341 tcaatgcagc agccttgctg aaagcatcat acgctttgtc gatattatta ttagccagat
     2401 gcgcttgtcc cgaatagtag tgcgcatcta ttcgttcttt atttgtaacc agatcgctgg
     2461 caaggatacg atttgcattt acaatcaccg catcaaaatc attgaggaaa taagcagtct
     2521 gcaataaccc taccatggct acaaaggtgt tttctttgaa atcagccact tcatacaatc
     2581 gctcataata gttgtatgct gtttgataat cttcattctg ataatattca atccaccctg
     2641 ctctcacgag tgctgattcc aggaatttag ttttatttcc tttaattata gattcaaaat
     2701 ccggcaatgc tttagcatat tgctttaatt gataatatga ttctccgcga tagaattcag
     2761 cttcagattt atatccacct ttagggaatt cgcgcaaata atcagagaag gctatagccg
     2821 ctcccgaaaa ctcgttcagg tcgtatttat ttttagcgta gttatatttt tcttcatcaa
     2881 tcttagtcac ttcaaccacg ccatcggcag cgccagtatt accggtttct tttacgatat
     2941 cggcaagtcc ttctttagct tgttgtcctt cttcagagtt tggagaactt tcgtaggcga
     3001 ggcgataata tttctcagag tttttgtaat ctttctcatt gaaataaatc aaaccgagct
     3061 tgagataggc cttaatggtg taactgctat taggtttttc acttactagt tccttgaacg
     3121 cagcaataga ctctgtgttc tttttagtga tgaagtatgt attggcaatt tcatacatag
     3181 catcatccac atacaatgaa ttcgggtatt tgttggttaa tgattgcagt gttgcaattt
     3241 taccattgct gttattttgt aacccctgca acatcgcttt ctgatacatc gcataatcaa
     3301 cccctttggc attgttgtcg attattttat tgtagtttgt ttctgccttc ttataatctt
     3361 tcgtcataaa atagcagtca ccaagacgaa gagatgcatc gatgtaaaca ttattttgaa
     3421 ccatatcggt tttactggtc ttcaaagcag tagttacttt gctgaaatag ttcaccgcat
     3481 ttgaataatt gctttgtttg aagtaagcat aaccgatagt ataatcgcta taaggaacac
     3541 gtgtttctga cggaaggtct ttggcaactt tagcgagatc ttgaaattta acgtgatttg
     3601 ttatggtgag tgcatattta ttctcagcaa acctgatctc gccaatccag aaataacatg
     3661 ctgcttgcaa attcacatct attggatttt ccagtgattg catgaacatt ttatcagctt
     3721 catcaaagtt tttttgatta aacaattgca caccggaata atacgacacc ttttgatagg
     3781 cttccttcaa ttttggtgtt ttttgaggga tgttttcaat tacatccatc gcagccaggt
     3841 aatcgtttgt accgagtaat gcattcgcca ataattcttg tgcttcggtt ttgttgcttc
     3901 ctttaggata tgccttcaaa taatcttgca ttgcagttac agcagcggta tgatatccgg
     3961 attcatatga aagcttgcca taattaaata atgcttgctc gtgaatggtt ttgtcttcac
     4021 caattcgcga agcttccata aatgcattac gtgcttcttc ttttttatcg agagcgatat
     4081 aacaattgcc atataaatat tgaatatgct gatacaaggt atcattatca tcgctagtct
     4141 cctttaatgt tgtcagagca gctttactgt cacctgtaga atactgtgca aatgccagtt
     4201 gataataatc ctcatcgcga atattctttg ccttttcaat gtaatagttc agatatggta
     4261 aggctttgcg gtaattattc cgatagaagt atgcctgacc tattaaactg ttaatttcgg
     4321 caaaatacac gagtttggta tcagccatca gcggttctgc atacgaaatc accttttcat
     4381 attttccttg ttgaaaataa atattggtga tgtaatatgg cacaactgca ttatatggcg
     4441 gcacatcttt tacgatgtta aaacaggtta acgcattttc atagttgcca tcttcctgct
     4501 gaataaatcc gtaataataa ttagcagcgt tataatattg attttgcggt ttgtctttta
     4561 atgatgcgaa catcactttt gcctcggtaa aacgtttttt gttaaacagg ctgtagccgt
     4621 attgaaattt gtaggtgtcg cgttcttcaa gcgaaagatc tttaacatcc accttttcaa
     4681 accaggttgc ggcattggta tactcttttt tattgtagta taatttaccg agttggttgt
     4741 aggccgaacc aagcattacg ttgccgggat tgttatgtac gaaatcgagc atcatttttt
     4801 cagcatcggt attgtttaac tcgatagcgc atatagcagc ataatagcgg ctattcgaca
     4861 cgtaggtgaa gatatcgccg tcagttccct cgggcgtaac cgagcctact tgttcaaagt
     4921 attcttgtgc ggtggcatac atcttattct catagagctc catcgccttt ttataattgg
     4981 catacagatc ggtataaatc agcgattgtt gtgccttcag tgctcctgta gtgccgcata
     5041 acacgatgca catacaagct attattagtt tgtttctgat cataagttgt tccccaaaag
     5101 ggtataatta tggctaatct aaaatcgcgc cgaaattacg gctattaaga gtagaagcgt
     5161 tcaatccatt gataaatgaa taaacaggtg tttgttgata atgaacgcct tagggagaaa
     5221 aaatagtatg tataggctat aacaaacagc agaattgcta ttctacaatt ttatctatcc
     5281 aggtcatatc gccataatag taccaccggt tgttaaattt cacagcggtg aattctacct
     5341 tacggggaga accatcagcg tcaaacttag tagtaacgcg tgcctttggc aatttgccga
     5401 gcttagattc agtgtaatta cctcgggtga aagttgcgga tgaccagtta ctcaccttat
     5461 tcctgactgt atccaaacac gctgaggatt gcttttcgaa tccgttgtat ccggcttcaa
     5521 ggttgactaa gatatgtcca ttttcctctt ctatctttct gaaagttcct ttatcaggta
     5581 atagcaatgc tattttttcc ggcgaattag atttcatagc gtcaaatagc tttttcgaca
     5641 ttgtttcggg gtccaatgtg ccgcgttccg gttcgttgca ggaaacgatt gccagtaaga
     5701 ggatcgggaa aataagtctt aatctcatgt gcgtggttgc gggttaaaag cggatgtaaa
     5761 atttatgtat tttctttacg tggacaaata cttggactta cggtaagtta aaaaagatta
     5821 aacgagtaag gaatggttca aatttataca gatggttctg caaggggaaa tccgggtccg
     5881 ggtggattcg ggactatttt gcggtacggg aagcatgaaa aggagttgtc gggaggttat
     5941 cgcagaacta ctaacaaccg tatggagctg atggctgtca ttgcaggatt agaggcgctg
     6001 acaaaggagg gacttgtcat caccatttac actgattcca aatacgtgtg cgaagccatt
     6061 gaaaagggat ggatttatgg ttggatgaag aagggtttcg ccggaaaaaa gaatgccgat
     6121 ctgtggactc gactgatgcg cgcttatgca cgtcataaag tgaagttgat ctggatcaag
     6181 ggtcatgccg gtcacccgga aaatgagcgc tgcgacgtat tggcaactac ggctgccgat
     6241 gggcacaagc tggctattga tgaggtgtac gagatggaaa accaaacgaa actaggattt
     6301 gattagtcta aactgatggt ttaacagtct aaactaacac cactctagtt tagactttag
     6361 cttcagtaaa cctatcgagg aactctacga ttttctccac catggccgaa gaaccaatgt
     6421 agaatggtgt tttctggtgc aactccttcg gttcgatgtc gaggatacgc tttcttccgt
     6481 ttgtagctac tcccccggcc tgttcggcga taaatgcgag cggattgcat tcgtagagca
     6541 aacgcagttt accttttggc gatttagccg tttcgggata gaggtatacc ccacccttca
     6601 gcatattgcg gtggaaatcg gcaaccagag acccgatata gcgactggta tacgggcgtt
     6661 gcgatttaag gtcttcctcc tgagtatatt tgatatagcg tttcacacct tcggggaagt
     6721 gaacatagtt tccttcgttg atggaataga tgataccgcg gttggggata cgaatatcgg
     6781 ggtgggaaag gcagaattca cctattgatg ggtcgagggt aaagccattc acgccaaaac
     6841 cggtggtgta caccatcata gttgatgacc catagatgat atagccggct gcaacctgtt
     6901 ctgtgccttt ttgcaggaaa tcctccaaag tagcgccacc gctgagactc agcctgcgat
     6961 agatgctgaa aatggtgcca atggacacat tcacgtcaat atttcctgag ccatccaatg
     7021 gatcgatgag cacgacgtac ttacctgatt ttgaaattgc accatcgaaa ctgatgaagt
     7081 tgtcttcctc ctcgcttgca aatccacata cttcacctcc ggccatcagg gcgttcatga
     7141 attggttgtt ggcatataca tccagctttt gctgggtttc accactcata ttttcgctgc
     7201 cttctgcgcc gagtatatca accaaccccg ccttattcac ctcacggttc acgatcttac
     7261 ctgctatgct gatatcggaa aggagtcggg tgagttctcc ggtggcaaaa gggaagtcct
     7321 gttggcgttc tacaatgaat tctatgagcg tttttacctt cataaagcgt ggttagggct
     7381 gcaaagttaa cacaaaatgt ccctgccccg atgattttac tcacctaaac gtagatttgt
     7441 gacatggaat atcacattcg ccctgccgat ggtgctgaca tcgccatgat acacaggtta
     7501 gtttcacaac tcgctgaata tgaacgtgct gcggatcagg tgatcactac tactgcagat
     7561 tatcagcgcg atttcgaggc cggttgtttt caggcattag ttgcggtgga cctgcaatcc
     7621 ggtgaaattg tggggatggc attgtattac tttgcatact cgacatggaa aggccgatat
     7681 ctgtggcttg aagattttat agtcactgag caatgcagag gtaagggagt tggtcgattg
     7741 ctttttgatg ctgtgggagc tattgcagcg aaggaaaaca catttatgaa gtggcaagtg
     7801 ctagattgga acacgccggc gattaatttt tacaataaat atccgatgga agtgcaggat
     7861 gaatggttaa cctgcagaat gccggtgcgt tcatgattaa taccggaaac ggtggcggat
     7921 tgtttactcc gtttcataca acacatcccg aagtatgggg atgatggtga gaaaatagag
     7981 cacatgaatg atgattattc tgaacatcca caaaatgaat acttcaccga gatcagataa
     8041 gtccaaatcg gcgttatcgg tatacattcc ggcaatccaa attacgggca ttgcgagtaa
     8101 aatcatccat gcgaagcgga attctttgtt tatggcgtaa atgatgatgc ccattatttg
     8161 gacaattgat ccgaaaatca tgatttccca ccaatcggga tggatgtttg gccttgtata
     8221 gaaaacaatg aaccatagat gacacatcat ccagcaagcc aattgcacga tactgtggta
     8281 tcgcatgaat gctgttggcc tgggttgatt tttggaaagt tgcttgcgga ggttcatggg
     8341 gaaattgggt ggtggtgggg ataaaattct gaaaaaaagt tggcaatcag tcgatgctcc
     8401 aaacctggcg cgttttaaac ctaaaaggtc ttaaacctaa caggttttaa acctaaaagg
     8461 tctaagacct aacaggtctt aaacctgtta ggtctgattt tgaagaactc cctcctacca
     8521 tgcttattac ccgaataatc gtttataagc gagtattcgc gataatttac aggcaagaaa
     8581 tacatgaatt cagaaatttc gaaataggct gcggtaaggt cacttgattc gcaaggtcaa
     8641 gttggcttca ctattattca tcctgatata atcccatgat tgtttatcat tcagattcgt
     8701 gtccaataaa actgaatcct tccaaattct tacatggtat gagaggtcaa ctgaatcctg
     8761 gttttcaaat cgatccacat agcctaaaaa acactgctga aataaaatct gctcggtacg
     8821 cttaggaatg cgtatttgtg taactttatt actgtcatat tcaaaatatg taacgtcaac
     8881 taccccattg ctttcattgg caattttaaa agtgatacaa gaaccggggt cgcaagcaat
     8941 cagaaataaa attaccggaa gaaacacgat cgctgcccga ctagcattat ttgaaaaaaa
     9001 gtgtcgcata ttttttcaag tatcaattaa atcatgcctt cctcagtatt ttctccatag
     9061 cccttccttt ggccaactca tcaaccaatt tatcgagata ccgaaccttc ctggttaatg
     9121 ggtcctgtat ttcctcaatt cgatatccgc aaatcatacc tgtaatgagc tttgcattag
     9181 gattgagttt cgccttatca aaaaaatcag caaaattgac acgcttttca attagctttt
     9241 gtaaagttac aaggttatac cccgtaagcc attcaatcac ctgatgcaat tcatccttag
     9301 tcctcccctt cttttcgatc ttagcgagat ataatggata tactgaagcg aaggtcatcg
     9361 ccgcgatacg tgcttcatgt gatttagttg ttgccatact cgggggttta tttattaaag
     9421 ataatgctta gcaataaatc tacaatgatg taaattatgt ccaacagtca taaagctatg
     9481 ttcatattaa aactacaaat aggcttaata ctttgaaaca gtcaacatac tattaatcaa
     9541 ataaacacca gcattgcctt acgtgcatct tgatatctat tgagggtttt ctgcaaagtg
     9601 cagaacattt tatacctcct aggctcacta aaatcacctt tcatgatgtg attgtcgtag
     9661 tcataccctg aagtagaaaa aatgctccgc ttcgctgcac attttgtcac ttcagggcag
     9721 gcactgagag gggaatattg tgctctgccg aaggcagagc gttctcctct tccaccacaa
     9781 gcctgccccg aagtgtaaat tttcgtagcg aagcggaaaa aaatttctac ctccggggtt
     9841 tcagcttaca gatttctctt ttgagaatta attagctcaa ttgcgtagct cattgaattg
     9901 agaactagtg cgcctgggta tatatcggga aaggaattag ggaaagaagc acatttcggt
     9961 taaggatcag gttactgtct tcttaatcaa attattgttt caaatcccat acttaaaatt
    10021 tcaaagcatg aacattcact taatttcaag aaccttcatg caaacaatga atcctatcga
    10081 aaccatggca accaaagccc accaaaccaa attcttagag gcatgatcca catttttact
    10141 tctgtaatat tggtggtacg aatatcccat caacgtcatt aaggcaatgc cggaaatggc
    10201 aacaaataac ctattttctg aaaggtacac cacagccatc aatccaattg atagcaaaga
    10261 aagcccacga cggtagagtt ggatacgact tgcgttgaaa ccaacagcaa taggatgcac
    10321 ctccgctagc agcacctcca aattggccat atcctccagt tgagcaggaa catgtatcac
    10381 atccaatttg gtgcggcccc gaatgataat atctcccgac aaatttttaa taatatccct
    10441 tacctcaatc cgaggtatcg tgattgaagg cgtgttgagc tgctctcttg taatatcctc
    10501 ctcattgatt gtaagcacat agctttcaaa tatcgctctt cgtttattgg cgacataata
    10561 ggtcactaac aaattgacac caataacaaa caatagaatc aacggcaaat agacgccggc
    10621 tatctcatcc caatcagcct tataaaaccc gatcagaaac cctcccacac cggctatgag
    10681 cgaaacaggg atggttttca gaagcaggtt ctttcttacc tgggggaaag agttgggggt
    10741 gggtttgaag tggggcatgg aaataaattc aaaatttact ttaatacaat tgatagtatt
    10801 acgattacct tcagctactc gcaactacaa ttgcttctgg aatgtatttc cataaattat
    10861 tgacattcct gccataaaac cattccgcct cattctgctt acaaaccata ttgtcctctg
    10921 ggttaaatgt tccaaccaca agaagcttat ttcttccaat aattgagcga cccgaaaagg
    10981 tttgttcctt ttcaagtttt attaactcat gaataaattt gtgttctatt gttttcataa
    11041 acttacctgt aacgttccga aggtaggcgc agtggccggt gagaaacgtt atagcgaagt
    11101 gatagctaaa ttacaaaaag gagtggaatt attctttcga ctggaaaccg gccattgtgc
    11161 ttaccttctg ttatagcacg tggttcattc gatgaccact tttttagagt aacttctttc
    11221 gttattcttt attgtgacca aataaacacc tttaggctga ttgcgcatat caatcgcatt
    11281 tgtgttttcg ataattgttt catgaaatac cgcttgccca gcaatattaa taattattac
    11341 atcgaaattg tcatttgata ttttatggat gtaaatattg tcttcaaatg tataaattga
    11401 aaaatccgga gatgaattgg ttgaggcaat atctacatat gaaatggtgt ctccggtaag
    11461 aattggctga ttgcattgtg tttcgtatgc aaagtcttta acaattaact tgtcaacaga
    11521 atccataact acacatcgaa tccagccata gtgaagacag ttttcctggt cttcaaagcg
    11581 aacgcctaaa tatctgatgg aatctaataa cagattattc caatttgcag gctgtaaatg
    11641 ccaatctccg acacctaaaa ttgtaaatga atagccataa taaatttcag cggttttaga
    11701 ggccattagt tggaatcctc cggcttgaaa ttcaaggtca ttgttaatga gtgcaccttg
    11761 attgagtgca tatggaaaat agtaggtgcc tcctgctgag ctattgtgca cagattctcc
    11821 ggcaatttga ggcccgacaa ctgcaggtcc gacccaaata gcaaccctgt aataccaagt
    11881 tgtatttaga cctggcgagg atagcgttga aataatattg gttgacttta ggaaaccgaa
    11941 ttcgtaaatt ccatcatcat tcatatctat atattggaag tcaccatcat cctgaaatac
    12001 caaatccggt tctatatccg tatatacaac ctgcgtttgt gcttgttggt gtaagagcag
    12061 gaagccggct gcaaaaccgg catactgctc tagtaagaat tttcctttca tgattactca
    12121 attttaatca attgctttga aattgtcaaa tctgtttcat cctttattgc caccaagtat
    12181 aaaccggaag gcaagtgaga aagatcaatt ggctgattct cagctgcttt tgtttcgaat
    12241 actactttat ctgttacgga gtaaactgtt accctaagct gcgcgtcaga gaataatcca
    12301 ctgatttctg atcgaatttg aaaaacacca gatgttggat ttggatataa ggttacaaat
    12361 tgggataagt cctcaacacc tgttttttgt tgcgggtcat aattaacatg gattggaatt
    12421 ttgaaatagc ctattgagta ttttggaacc gttatacaca tcgctcctgc aggtaatcca
    12481 tctgaacatt ccgggtcatt tggaattggg tctgaaattc tttgaatctc aaacgggtgc
    12541 gggttatcaa gaccatcaaa caagttatga caactgtagc atgtatgtat tttaaacaaa
    12601 gtacttaacc ctgaattaga ataaggctga ttggcttgaa ttgtataaat agttgcatct
    12661 tcaaacccca aggaaacagc ggtagggtat aggtccatta aatgattagg atttacagcg
    12721 taactctgtg tttcagcttt catattagtg aaatacacat acaaattttc atgttctggg
    12781 tcaatgaaca cagtgggttg taaattcggg ttcgtaaaat acattgaaaa attagccggc
    12841 aaatattgca agttgttctt ggttatctca ctaagcagtt ccatgacata atattcagtc
    12901 cgacgcatat aatagtttac gtcgccagta tacagagcag gatttagcgg attaggagag
    12961 ccatcatcat tgatatgatt tttgcagtca gcgcaatctg caatgcataa catatctgtc
    13021 cacccacctc cggcaaaatt atgaaatgta gaataggtga aaaatgatgg tatataccct
    13081 aaagtatagt tcaacttaat gttttttaat actaattcct gtgttataag tccatgcgga
    13141 aagctattgt tgtagatgct gattaaatcc tgttcctcgt ctgatatttc attactattt
    13201 accttgtcct ttaggttcca ttcagtgttc cacaactcct taggatatgg atttgatggc
    13261 gaaaatttta agactgcttt ttgcttgtca taatattcca aataccttgt ttttaatgcc
    13321 gttttaaaat ttccaaatgg tggggctaat gtagttccca atattccgga ataggcggcc
    13381 accaagcgag aatcataggt accatattgc caattggagc cagtcggaca accttccgca
    13441 agattacatg atggaaaacc gtcgctaggg taaaacagac aaagattgtc taatggtatc
    13501 tgtttgtaat tatgggttgc gtcgtaatag gtatgtagta tcacagcatc gaacttatag
    13561 cgggtttcag ttccggcagt aatagtagag ccatagatta caggattggc caaagaactg
    13621 ttccaacctg tagtgattgg ataccccttt tcagccccct ccctgaacat atacgttatg
    13681 gtttctgtgt tttgtggatt atccgcaggt attcccactt tgcaattaaa gtctatgtct
    13741 cctttaaact gattgatgta attatgactg gttttcacag gcaaatcacc aaatatataa
    13801 tttctttcat cagcagtaag tgctgagttg cctccattga tatagttcca gtaatcacca
    13861 aatacgtcaa atcccatcag atcagcgccc caattcaaat aacactcatt gccaatttct
    13921 acaccaacaa cattcacatc tgttactccg ttttttgtgt gatcgcgcat atatttgaca
    13981 attgccttgc attgagccgc tgattcacta agtatgttaa gatctagaat cacatcaatt
    14041 ttatagccgt gagcggtttc gatttcattt actaaatcta tgaattgatt tatgtattca
    14101 tgccttgggt cggttacagg aaaaggaatt tcatcttgtg tcgcccattt cccataataa
    14161 ccttcaaggt ctttctggta tttaacagca tccatccact catgacagta atcacaatct
    14221 ccatcgcttg ttagatctgt tttaattgag cttaaatatg ctggttcatc cgcttgaata
    14281 ttatcaatga tatcatctgg gtcatcggtc tcatttgtaa t
//
LOCUS       DAOQHY010000082        14245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2040_length_14245_cov_5.708386, whole genome shotgun sequence.
ACCESSION   DAOQHY010000082 DAOQHY010000000
VERSION     DAOQHY010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14245
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2040_length_14245_cov_5.708386"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="PLL28_08165"
     CDS             complement(<1..1106)
                     /locus_tag="PLL28_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF69339.1"
                     /translation="MQRLPALLLLIACTHTVVIHAQFCNDTRFTEAEYFSDSEIETNY
                     NVSYGLASQWFMDLPQPSLNTLDIAFPDQTVDPMEKRPLIVLVHGGGFWGGEKESFSY
                     HLQELAKNGYVVAAINYRKGWMGSPLDCDGDPNSLSLAIYRGMQDTHAAIRFLVANAD
                     VYGIDTANIFAGGESAGVYAVMNAYYLTQDDWESYNPGYGYLYGDINASTNEYTTTFK
                     LKGILNMWGGMVDTAFVSVNEAVPSIAFYGMSDGVIPPGHGNIQYCENFAEIFGALGL
                     SEFYNNNNICNVLHQNPTEGHEAYPADYVCPNIACFIKGVLCNTCSTELMHYEISDCS
                     ADVVIDTTTDTTETLNIEIAQIETLSLFPNPADQR"
     gene            complement(1361..2281)
                     /locus_tag="PLL28_08170"
     CDS             complement(1361..2281)
                     /locus_tag="PLL28_08170"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNF69340.1"
                     /translation="MRILLFSLLCSMMLAGSVQAQDTLKQGLIQVNDTHLFVKIMGKG
                     DTILVIHGGPGLNHGYLEPHLLPLAKTHTLIFYDQRSSGESQLSVKSHMNFATFADDI
                     EAIRKHFNINHLNLLAHSWGCLVAATYMLNYPEHVQSVVFSNPVPFDHTMAEMSAQTA
                     ADRETPADSIHKAQLIASDAFRNGDVEPVEELMKTSFKLLFCDTTNLSLLDPSLPSSY
                     MVASLSLYGFMDDMKNYNFFPKMKDIPIPALIIHGNCDISPLDADLRLQKCFTNADVT
                     IFENSGHFPFIEENKKFNKTVSKFFLDHAK"
     gene            complement(2336..3724)
                     /locus_tag="PLL28_08175"
     CDS             complement(2336..3724)
                     /locus_tag="PLL28_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNF69341.1"
                     /translation="MYIQQLYTNCLAQAAYYIESEGEAVIVDPLRDVQTYIDLAASRN
                     AKIRFSLQTHFHADFVSGHLELARKTGAVIVYGPGAQPDYAAAVLKDYDVLDIGKVKI
                     MILHTPGHTIESTCFLLFDEENNVHSIFSGDTLFIGDVGRPDLLSGNLDAETLGGMLF
                     DSLQNKIKVLPDDCIVYPGHGAGSACGKNLGKETTSTIGIQKATNYALQFTDKQAFID
                     AVIAEQPAAPAYFFKDARINIHGYESLDKVMDAAMKGIDAATVQLETAEGALILDTRP
                     AAVFAKGFIPGSLNIGLNGEFAVWAGTLVDISTPLILISEEGKEKEAVTRLARIGYEN
                     ILGYLEGGFASWKSEKLPTSTIKNVSVDDFANLMDSGKYALLDVRKPGEVEKNKVFEA
                     HHISLNKLAAEMDTLDPKNKYIVYCGGGYRSMMACTMMLRAGIKHVVNVAGGINAILK
                     EKPDLVQYFELV"
     gene            complement(3842..4972)
                     /gene="hppD"
                     /locus_tag="PLL28_08180"
     CDS             complement(3842..4972)
                     /gene="hppD"
                     /locus_tag="PLL28_08180"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HNF69342.1"
                     /translation="MDTIVLDQKKTNVQEDFLPLNGTDFIELYVGNAKQSAHYYKTAF
                     GFQSLAYSGLETGNRECASYVLRQGKITLVLTTPYHSDSPVAEHVRRHGDGVKVIAFW
                     VDDAEATFEKTIARGAEPFMYPEVRTDDTGEVKIAGIKTYGEVIHLFVERSNYNGVFM
                     PGFVKWESEYNPTPIGLEYVDHCVGNVGLGRMNVWAKFYEEVLGFKNLITFDDKDIST
                     EYTALMSKVMVNGNMRIKFPINEPAKGKKVSQVQEYLDFYEGEGCQHIAVATDDIIHT
                     ITEFRKRGVEFLYVPGTYYDTVLDRVGKIKEDLEVLKSLGIMVDRDEEGYLLQIFTKP
                     VEDRPTLFFEIIQRVGAKSFGKGNFQALFESIEEEQRKRGTL"
     gene            complement(5028..6191)
                     /locus_tag="PLL28_08185"
     CDS             complement(5028..6191)
                     /locus_tag="PLL28_08185"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HNF69343.1"
                     /translation="MYHQLGKIPNKRHVVYRNSEGQMYNEELVGTQGFSSLSTLAYHI
                     YPPTRVIGTGTPFDMRPRIAIENNMRMLSFSGFDIPAEEDYLQSRKVLFVNSDLHIGL
                     AAPTQSPEYFFKNADADELIFIHKGSGTLSTMYGEVEFEQHDYLVIPRGTIYKIDFDT
                     DDNRFLFIESFSPIYTPRRYRNEFGQLLEHSPFCERDFKKPANLITHDEEGEFEILIK
                     KRGQVFPYTYANHPFDVVGWDGYLYPYAFNIHNFEPITGRIHMPPPIHQNFEGNNFVV
                     CSFVPRLYDYHPQAIPAPYHHSNIDSDEILYYVEGEFMSRNNIKQGQLTLHPAGLPHG
                     PHPGAIERSIGKKETHELAVMIDPFKPVSITETAVELEVEDYYLSWISKQLTH"
     gene            6537..6923
                     /locus_tag="PLL28_08190"
     CDS             6537..6923
                     /locus_tag="PLL28_08190"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HNF69344.1"
                     /translation="MSFSAKRARELRRRQTQAEAIMWKELRKYRKIKFPFRRQHPVTL
                     VEISNSTILYVLDFYCMPIKLAIEIDGPVHLQSEVIHYDKHRQSLLEEMGIMVVRFTN
                     DEVYHNLEYVIKTIEDCIATRIKGLS"
     gene            complement(7204..8577)
                     /locus_tag="PLL28_08195"
     CDS             complement(7204..8577)
                     /locus_tag="PLL28_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNF69345.1"
                     /translation="MAFKTIIEPFRIKTVEPIKMSSVAERKKYLKAAHFNPFLLHSSQ
                     VIIDMLTDSGTSAMSQNQWAAIMQADESYAGAQSWERFYAAVSDLTGCEFVLPTHQGR
                     AGERILYGYLGGKGKVFISNTHFDTTRANIEFSGAEAFDIPCKEADDFDSNHPFKGNM
                     DVEKLEKLLKQHKKNVAAVIMTVTNNSGGGQPVSMANMESVSALCKKYKVLLILDCCR
                     VAENAWFIRHKEPGFENMSYRDIAKKMFDMADGSVMSAKKDALVNMGGWLTLRDKKIA
                     DACMGLLIITEGFSTYGGLSGRDMEAVAVGIKEVFDDDYLNYRIKSTAYLGEHLRNNN
                     IPIMYPTGGHAVYVDAAKLYPHIPVHQYPGQALACALYTIGGVRSVEIGSVMFGKYDN
                     KKKLIPARHELVRLAIPRRVYTQSHIDYVIEVFREVEKNKLRAKGLKIIYEQAFLRHF
                     TARFAEV"
     gene            complement(8773..11304)
                     /locus_tag="PLL28_08200"
     CDS             complement(8773..11304)
                     /locus_tag="PLL28_08200"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNF69346.1"
                     /translation="MMKAHRLLLAFLSIVVCSLSMHAQTPEKPNIVFIILDDMNDFTG
                     TLGGQPQIKTPVMDSLFGLGTNFMNAYCNAPVCGPSRTSMLSGKDMKYTQVYDNNEYL
                     DEFRDNFTTELGNEEVITIPEYLKNSGQYYTCGINKIFHDPFNKDYDVDTEDPCLKTL
                     SWSKVISFSNWDSTTDKLEATNVGVDGYLWGVVDSAVEYELKDYRAVDSAIEFINDVA
                     SGEIALCDSVFFLALGFAMPHLDLYVPEGYFPADYLNDIYEEPFNYPYNFPENTYPFN
                     GIVMPPQPDPKWNDYYALGPLGHAISIGQKDVETSFSDYVDSLPMLPVIEPDLTDEDR
                     KEILMEAKRANAIMAYMAGVQFVDAQIGRLMDSIQLYPEIYNNTVFIIVGDNGFSFGE
                     KHHWLKRSLWETDIRVPFAIFGPSFPQHQVSYNAVSLLDLFPTICDIAGLPYPSFANG
                     EPYLDGKSIFPILNNASIAFAHPALISFEAENNKECSCFPQIAVRDDRFAYIRYQSDG
                     GDPVHDCILDSSVVEEELYEVGVHRETDPNEWNNLIDNPDYAPVVNYLQQFLPDSNLY
                     LKTAYKVVITNNSLACLLEHNDTLFLAFDWFDENGIAIAPDPGYVYKWTNNLTDDILI
                     GTSVNFMCNWISDDDFNAGNRLMIYLQVYDTSGALIAFDTKYFYVNPDFAPTCSFEMF
                     GDGELTVSFTDLEITGNYTCYWWEINGDSMFYNSMPGPFTFEASGAYAITLHVQYGNT
                     PCIKSFTRTFISDEIQTNNIDNFLVYPNPANTILRIEFPNGAYDGHLELSDINGKIVR
                     SVELNADSILYYDIDVANLASGLYVLHFYGNATEFGTPVVIMHNK"
     gene            complement(11308..13212)
                     /locus_tag="PLL28_08205"
     CDS             complement(11308..13212)
                     /locus_tag="PLL28_08205"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNF69347.1"
                     /translation="MSKSLKCFVCMLFVIQATNGFAQTLPYQLNYFEGTHHMTVGGNP
                     PSDLYDINVIREINLLFYVDDWYDQLLDTHGTELAIPARLVYNGDTLPADVGVYFRGH
                     SSFFANDSIKKSVGITLDYIDSLQNIDGSKTLNLNCSFRDTTFMHEAIYENLIQKYIP
                     ALSVNYVHLYLNDLDFGLYTNVQQLDKIYLKDWFITNDGSLWRAERTDGATDADPGGA
                     NGTGFSTLNYLGMDTSVYKNYYTLKETSISHPWEDIIDVCDPLENMPADVLEDTINKY
                     LDLDRTLWYMAAEILFTDEDSYVHKGGMDYFLYWEPETDRLVPLEYDGNSVMDSMRYK
                     WKIFYKGTDTNYALSYHFFAIPSIRQRYLAHFRTMFNEIYTEENLYGIIDDYFELIDP
                     YVATDPVKLVSYDQFLYGIDQLKETIANRRAYILDDNEFDNPSLNIENVKWISNGYNW
                     GVVNSIQPVTISAQISGAPDVKQITLYYSTEIFGPFTKLIMYDDGLHGDQLAGDLIFA
                     SAIPNFPVGTKVRFYVEATADDDVFTKSYNPEGAEHDVYYYEVKTDYIEVNNFTNNYF
                     TLLPNPTSSLLYVHCGNPSNENLEIYSITGAKVFEAKLTVDMEINVATWSPGLYVAKT
                     TEFAYQIIVQ"
     gene            complement(13361..13612)
                     /locus_tag="PLL28_08210"
     CDS             complement(13361..13612)
                     /locus_tag="PLL28_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNF69348.1"
                     /translation="MCCCYILHSITLDRYYIGHTCEDVQERLRRHLSDHEGFTGKAKD
                     WKIVYTEGFEDKSAAYHREQEVKGWKSRKRIAQLVQSSS"
     gene            complement(13702..13956)
                     /locus_tag="PLL28_08215"
     CDS             complement(13702..13956)
                     /locus_tag="PLL28_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNF69349.1"
                     /translation="MCCCYILYSITLDRYYIGHTCEDVQERLRKHLSDHEGFTGKAKD
                     WKIVYTEGFEDKSAAYRREREIKGWKSKKRIAQLVRSIPL"
     gene            complement(14014..14088)
                     /locus_tag="PLL28_08220"
     tRNA            complement(14014..14088)
                     /locus_tag="PLL28_08220"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14051..14053),aa:Val,seq:tac)
BASE COUNT         4319 a         2985 c         2747 g         4194 t
ORIGIN      
        1 cgctgatcgg caggatttgg gaacagtgac agggtttcta tctgcgctat ttcaatattc
       61 aaggtttcgg tagtgtcagt tgtagtatca atgacaacat cggcggaaca atcagaaatt
      121 tcgtagtgca ttaattccgt actgcatgtg ttgcaaagca ctccttttat gaagcaggca
      181 atatttggac acacataatc tgcagggtaa gcttcatgtc cttctgttgg attttgatgc
      241 agtacattac aaatattatt gttattataa aattctgaca gtccgagtgc accaaatatt
      301 tcggcaaaat tttcacagta ttggatattg ccatggcccg gaggaataac gccatcagac
      361 ataccataaa atgcaatgga tggcactgct tcattaacag aaacaaaagc tgtatcaacc
      421 attccgcccc acatattgag gatgcctttc aatttaaatg tagtggtata ttcatttgtt
      481 gacgcattga tatcgccata taaataacca taaccgggat tatagctttc ccaatcgtcc
      541 tgcgtcaaat aataagcgtt cattactgcg taaactccgg cactttcacc tcccgcaaaa
      601 atatttgcag tatcgatgcc atacacatca gcattagcaa caagaaaacg tatggcggcg
      661 tgggtatctt gcataccgcg ataaatggcg agcgacaatg agttaggatc tccatcgcaa
      721 tcaagcggtg aacccatcca accttttctg taatttattg ctgcaacaac atagccattc
      781 tttgccagtt cttgaagatg ataggaaaaa gattcttttt caccacccca aaagccacca
      841 ccgtgcacca acacaataag cggacgtttt tccatcgggt caacagtctg atcgggaaat
      901 gcaatatcta atgtgttgag acttggttga ggcaaatcca tgaaccactg gctcgcaaga
      961 ccgtacgaaa cattataatt tgtttcgatt tcggagtcgg agaagtattc ggcttccgta
     1021 aaacgggtgt cgttacaaaa ttgggcgtgt attactactg tgtgtgtgca tgctatgagg
     1081 aggaggagtg cgggtaatct ctgcatacaa atgttaaaat gttaaaccat agtcgtgtgt
     1141 gtgtatggtt ttataagaac gaataacaaa aatacggaag acagtaagaa caattgctac
     1201 tgaagtaagg ttttcatgac atggtaactg ttacaacaaa tttgccgtcg aacagttcaa
     1261 ttttgatgag gtaccgaaag ctaaacttgg caataattta aactaaatcg tatacatgct
     1321 gcgagactag ggagtcagat aacacagatg aggaaattcc tcatttcgcg tgatctaaaa
     1381 agaacttcga cactgttttg ttgaactttt tgttctcttc gatgaacgga aaatgtcctg
     1441 aattctcgaa aatagtgaca tcggcattgg taaaacattt ttgcagacgg agatcagcat
     1501 ccagagggct gatatcgcaa ttaccatgga tgattaatgc cggtatcggg atgtccttca
     1561 ttttgggaaa gaagttatag tttttcatgt cgtccatgaa accataaagc gaaagtgagg
     1621 ccaccatgta ggaggaaggc aacgaaggat ccagcaagct caggttggtg gtatcgcaaa
     1681 aaaggagttt aaaagaggtt ttcattaatt cttcaaccgg ttcaacatcg ccatttcgga
     1741 aggcatcact tgcaattaat tgagccttat ggatactatc ggccggcgtt tcccgatcag
     1801 cagcggtctg tgccgacatt tctgccatgg tatgatcgaa aggcacggga ttgctgaaaa
     1861 caaccgattg cacgtgttca ggataattca acatataggt tgccgccaca agacatcccc
     1921 aggaatgcgc cagaagattg agatggttga tattaaagtg ctttctgata gcctcaatat
     1981 catcggcaaa agtggcaaaa ttcatatgcg atttaacact caattgcgat tctcctgaag
     2041 agcgctgatc atagaaaatg agggtatgcg tttttgctaa tggcagcaaa tgcggttcaa
     2101 gatatccatg gttcaagccc ggacctccat gaataaccag tatggtatcc ccttttccca
     2161 ttattttgac aaacaggtga gtgtcattga cttgaataag accttgtttg agggtatcct
     2221 gagcttgaac agaccctgct agcatcatgg agcagagcag cgaaaagagg agtatgcgca
     2281 taaaaaatcc ctgccgggtt atgggcaggg atgtaaagtt atacgataag tgttatcaca
     2341 ccaactcgaa atattgcacc agatcaggtt tttccttcaa aatggcattg ataccgccgg
     2401 ctacgttgac aacatgctta attccggcac gcagcatcat ggtgcacgcc atcatacttc
     2461 tgtacccgcc gccacaataa acgatatatt tatttttagg atccaaggta tccatttcgg
     2521 ctgcgagctt attcaaagaa atatggtgtg cttcaaacac tttatttttt tcaacttctc
     2581 cgggtttgcg cacatccagc aatgcatatt ttccgctatc catcaaattg gcgaaatcat
     2641 ctacgcttac atttttaatg gttgatgtcg gcaatttttc agatttccat gatgcgaatc
     2701 cgccctccag ataccccaga atattttcat agccaatgcg cgccaatctg gttactgctt
     2761 ctttttcttt tccttcttcg gaaattaaaa tcagtggggt agaaatatcc accagtgttc
     2821 ctgcccatac tgcaaactcg ccattgaggc caatattcag tgaacccgga ataaatcctt
     2881 ttgcaaaaac agcagcaggt cttgtatcaa gaatcaaagc tccttcagca gtttctaact
     2941 gtacagttgc cgcatcaatt cctttcattg cagcatccat tactttatcc aaagattcat
     3001 atccatgaat atttattctg gcatctttga agaaatatgc aggtgctgca ggttgttcag
     3061 caataacggc atcaataaat gcttgtttgt cggtaaattg caatgcatag tttgttgctt
     3121 tttgtatgcc gatggtactg gtagtttctt tacctaaatt cttgccgcag gcacttcccg
     3181 caccatgacc gggatataca atacaatcgt cgggcagtac tttgatttta ttctgcaaag
     3241 aatcgaacaa catgccacct aaagtttcag catcgagatt accgctaagc aggtcaggac
     3301 gaccaacatc accaataaat aaagtgtcgc cgctgaaaat gctatggaca ttattctcct
     3361 catcaaacaa caaaaaacat gttgattcga tggtgtgccc gggcgtatgc aggatcataa
     3421 ttttcacttt cccgatatcc agcacatcat aatctttcaa cactgctgca gcataatcag
     3481 gttgtgcacc cggaccatac acaattacag ctcctgtttt tcgtgcaagt tccaaatgac
     3541 ctgaaacaaa atcggcatgg aaatgtgttt gcagactgaa gcgaattttt gcatttcttg
     3601 atgcagcaag atcgatataa gtctgcacgt cgcgcaatgg gtcaacaatt acagcttcgc
     3661 cttcgctttc tatataatac gctgcctgtg caaggcagtt tgtataaagt tgttgaatgt
     3721 acatgtgaaa gaattttaat gatgatcgaa tatttgcatc tgcaatctta gatgacttta
     3781 tcaaaaatga gatacgtcct aaaaggcatc aggcagccac taaggactgc ctgatgttgt
     3841 atcacaatgt acctcgtttt ctttgttctt cctctatgct ttcaaataaa gcctggaagt
     3901 ttcctttacc aaatgatttt gctccaacgc gttgaatgat ttcaaaaaat aatgtaggtc
     3961 gatcttcaac cggttttgtg aaaatctgca gaagatatcc ttcttcatct cggtcgacca
     4021 taattcccaa gctctttaaa acctccaggt cttccttgat ctttcctacg cggtctaaaa
     4081 ccgtatcata ataagtaccg ggcacataca agaattcaac accgcgcttg cgaaattctg
     4141 ttatagtatg aatgatatca tcagttgcta ctgcaatgtg ctgacaacct tctccctcat
     4201 aaaaatcgag atattcctgg acctgactaa ccttttttcc ttttgcaggt tcgttgatag
     4261 ggaattttat gcgcatattg ccattcacca tcaccttgct cattaaggca gtgtattcgg
     4321 tggaaatatc tttatcatcg aaggtgatca ggtttttaaa acccagtact tcttcataaa
     4381 atttagccca tacattcatg cgaccgaggc caacattacc tacgcaatgg tctacatatt
     4441 ccaaaccgat aggcgttgga ttatattcgg actcccattt cacgaatccg ggcatgaata
     4501 cgccattata attactgcgc tcaacaaata agtgaattac ttcaccatat gttttgatgc
     4561 ccgcaatttt tacttcaccg gtgtcatcgg tgcgcacttc aggatacatg aatggttccg
     4621 ctccgcgtgc aatagttttt tcaaaagttg cttctgcatc atccacccaa aaagcgatta
     4681 ctttcacgcc atcgccatgt cgacgaacat gctccgctac agggctgtcg ctatgatatg
     4741 gcgttgtaag cacaagagtg attttacctt gacgaagcac ataggatgca cattcgcggt
     4801 tgccggtttc aagtccggaa taagcaagac tctgaaaccc aaaagctgtt ttgtagtagt
     4861 gagccgattg tttggcatta cctacataca attcgataaa atcggttcca ttcaatggca
     4921 gaaaatcttc ctgcacattt gtttttttct gatctaatac gatagtatcc atagttgttt
     4981 atttcaggtt tatcccttac agaaaataat ttactgctca ggaattttta gtgggttagt
     5041 tgttttgaaa tccatgataa atagtaatct tccacttcaa gttctacagc agtttctgta
     5101 attgaaacag gcttgaaagg atcaatcatg acagcgagtt catgtgtttc ttttttaccg
     5161 atacttcttt cgattgctcc cggatgcggg ccgtgtggca atccggcagg gtgtagtgtt
     5221 aactgtccct gtttgatatt attgcggctc ataaattcac cttccacata atataatatt
     5281 tcatcgctgt cgatgttgct atgatggtaa ggtgcaggaa ttgcctgtgg atgataatca
     5341 tacaagcgag gaacaaatga gcataccacg aaattattgc cttcaaaatt ctgatgtatt
     5401 ggcggaggca tgtgaatgcg acctgttatt ggctcaaaat tgtgaatgtt aaacgcatat
     5461 ggatataaat atccatccca acctacaaca tcgaatgggt gattggcata agtatatgga
     5521 aatacttgtc cacgtttttt aatcagtatt tcaaattcgc cttcttcatc atgtgtgatg
     5581 aggttagcag gttttttgaa atcgcgttcg cagaatggtg aatgttcaag caactgacca
     5641 aattcattgc ggtagcgacg aggtgtatat attggactga acgactcgat aaacagaaat
     5701 ctgttatcat cagtatcgaa gtctatttta taaatggtac cgcgagggat aaccaaataa
     5761 tcatgttgtt cgaattccac ttcgccatac atggtactga gcgtaccgga gcctttgtga
     5821 atgaatatca gctcatcggc atctgcattt ttaaagaaat attcaggcga ttgcgttgga
     5881 gctgcaagtc cgatgtggag atcgctgttt acgaacaaca cttttctgct ttgcagataa
     5941 tcctcttcgg caggaatatc gaaaccgctg aaacttaaca tgcgcatatt attctcaata
     6001 gcgatacgag gacgcatatc aaatggtgtt ccggtgccaa tcacccttgt tggaggatag
     6061 atatgatagg caagtgtaga aagactgctg aatccttgtg tgccgaccaa ttcttcattg
     6121 tacatctgac cttcagaatt gcggtaaaca acatgtcgct tattcgggat tttgcccagt
     6181 tgatggtaca ttggcatagg tatgactatt tatagtacaa atttccctgt atacccaccc
     6241 ttgcgaggtg acttatgtca atgttggcgg tgatgtttga acgagaaacg ctacgggtga
     6301 aagtcaccga ttggagtaaa agtgttgtgt gcttggttcg cgctaagacg ctaagttgcg
     6361 aaaacgcaaa gcatgcattg ggcctgacga tgtctgatat caatactaat aatatccggg
     6421 cggttaaccg atgatcaatc cacaggctga tttaagtata gaaagtctaa taagcggtta
     6481 gtgtggatat tttcggtcag aagtaccaac attagtaata aatatacacc aaaaaaatga
     6541 gttttagcgc aaaaagagcc cgagaattaa gaagaagaca aactcaggca gaggctatta
     6601 tgtggaagga attaagaaaa tatagaaaaa tcaaattccc attcagacgt cagcaccctg
     6661 tcaccctagt agaaatatca aactcaacta ttttgtatgt gcttgatttc tattgtatgc
     6721 caattaaatt agctattgag attgatgggc ccgtccactt acaaagtgaa gttattcatt
     6781 acgacaagca tagacaatcc ttacttgagg aaatgggaat aatggtagtt aggtttacaa
     6841 atgatgaagt ctatcacaat ctagaatatg taataaagac aattgaagat tgtattgcaa
     6901 caagaattaa gggactatca tagtatcagg gaatgcgagg gagtagtggg acagttaatt
     6961 atcattttct gatggaggat tgtggtagta gtagagaatt accgccgccc tcaccccgac
     7021 cctctcccag ggggagaggg agtaaccctt gattctaagc taaatttatc atggatacga
     7081 caaatacccc ctctccccct gggagagggg cggggtgagg ggcgacaagt tacctagcaa
     7141 ggtaaaacat aatctgtttg aatatcccta atgcatcaca aaactcacac aacacaatat
     7201 ctctcacacc tccgcaaacc gcgctgtaaa atgcctcaga aaagcctgct catagatgat
     7261 tttcaggcct tttgctctga gtttattttt ttctacttcg cgaaatactt cgatcacata
     7321 atcaatatga ctttgggtgt acacgcgtcg cggaatggcg agacgaacga gttcgtgacg
     7381 tgcggggata agtttctttt tattatcgta tttgccgaac atcacagagc caatttccac
     7441 gctgcgcacc ccaccgattg tgtacaaagc acatgcaagc gcttgtccgg ggtattgatg
     7501 tacaggaata tggggatata atttagccgc atcaacatac accgcatgcc cccctgtcgg
     7561 atacatgata ggaatattat tattgcgcag atgttcgccg agataggcgg tgcttttaat
     7621 tctataattc agataatcat catcaaaaac ctccttgata ccaacagcaa ctgcttccat
     7681 atcgcggcca ctgagtccgc cataagttga aaaaccttcg gtaataataa ggaggcccat
     7741 acaggcgtcg gcaatttttt tatcgcgtag tgtcagccaa ccacccatat ttacgagtgc
     7801 atctttttta gcactcatca cagaaccatc ggccatatcg aacatttttt tggcaatatc
     7861 gcgatacgac atattttcaa aaccgggctc tttatgtctg atgaaccacg cattttcggc
     7921 aacgcgacaa caatcaagta tcagcaatac cttatatttt ttgcagagcg ccgacacgct
     7981 ttccatgttt gccattgaaa ccggctgacc accaccgcta ttattggtta cggtcataat
     8041 tactgctgca acatttttct tgtgttgctt caacaatttc tctaatttct caacatccat
     8101 attccctttg aaagggtggt tgctgtcgaa atcatcagcc tctttgcaag gtatatcgaa
     8161 agcctcggcg ccgctgaatt cgatatttgc tcttgtggta tcgaaatgtg tattgctgat
     8221 aaatactttt cctttcccac ccaaataacc gtataaaata cgttcccctg ccctaccctg
     8281 atgcgttggt aatacaaact cgcaaccggt aaggtcgctc acggcagcat agaatcgttc
     8341 ccagctttgc gctcctgcat aagattcatc agcctgcata atggccgccc actgattctg
     8401 actcatagcc gaagtgcccg agtccgtaag catatcaatg atcacctggc tggaatggag
     8461 caggaaagga ttgaaatgag cggctttcag gtattttttc ctttctgcaa cagagctcat
     8521 tttaataggc tctacagtct ttattctaaa aggctcgatg atagttttga atgccataat
     8581 acaaatttgt agcgcaaaga tgaggcgaat gcagtttagc cggggtgact tatgtcaatg
     8641 caaagtaatt tgaaagcgaa gcagtgccta tcatgtaatc atgcaaaacg attgctatct
     8701 acacttgatt tcacaatgtt tcgaagaatt ttaattgatt tgagttacgc agagtattcg
     8761 aaaaagcaaa tcctatttat tgtgcataat cacgacagga gtaccaaatt ctgttgcatt
     8821 tccataaaaa tgaaggacgt ataatcctga agccaggttt gcaacatcga tatcataata
     8881 tagaatggaa tcagcattca attcaacaga ccgaacaatt tttccgttta tatcagataa
     8941 ttctaaatgt ccgtcatatg caccatttgg aaattctatg cgaagaattg tatttgccgg
     9001 attaggatac accagaaaat tatcaatgtt atttgtctga atttcgtcgc taataaaagt
     9061 ccgtgtaaag gatttgatac atggtgtgtt gccatattgc acatgcaagg taattgcata
     9121 agcaccggag gcttcaaaag taaaagggcc tggcatcgaa ttgtaaaaca tgctatcgcc
     9181 atttatttcc caccagtagc aagtataatt tccggtaatt tctaaatctg tgaacgagac
     9241 agtcaattcc ccatcaccaa acatttcaaa agaacaggtt ggagcaaaat ccggatttac
     9301 ataaaagtat tttgtatcaa atgcgatcaa ggctcctgaa gtgtcataaa cctgcagata
     9361 tatcatgagt ctgtttccgg cattaaaatc atcatctgaa atccagttgc acataaaatt
     9421 cactgacgtg cctatgagta tatcatcagt taaattattc gtccatttat atacatatcc
     9481 ggggtcaggt gcgattgcaa tgccattttc atcaaaccaa tcaaacgcca aaaaaagtgt
     9541 atcattatgc tcgagtaggc aagcgaggct attatttgtg ataacaactt tatatgctgt
     9601 cttcaggtaa agattactat cgggcaaaaa ttgttgcaag tagtttacta caggcgcata
     9661 atcgggatta tcgattaaat tattccattc attaggatca gtttctctat gcacacctac
     9721 ttcatatagt tcctcttcaa ctacacttga gtctaaaatg caatcatgca ccggatcacc
     9781 gccatcagac tgataacgga tatatgcaaa tcgatcatct cgcactgcaa tttgaggaaa
     9841 gcaggagcac tctttattat tttccgcctc aaagctaata agagcaggat gtgcaaatgc
     9901 aattgaagca ttatttaaaa tagggaaaat actcttacca tccaggtaag gttcaccatt
     9961 tgcaaatgat ggatacggaa gacctgcgat atcacaaatt gttgggaata aatcaagcaa
    10021 actcacagca ttgtagctaa cctgatgttg aggaaaagag ggtccgaata ttgcaaaagg
    10081 gacacgaata tctgtttccc ataaactgcg ttttagccaa tggtgttttt ccccaaagga
    10141 aaagccattg tcgccaacaa ttatgaaaac agtgttatta taaatttccg gatataattg
    10201 aattgaatcc attaatcggc ctatctgtgc atccacaaac tgaacgccgg ccatgtaagc
    10261 cataattgca tttgcgcgtt ttgcttccat taaaatttcc tttctgtcct catccgtcaa
    10321 atcaggctct ataaccggta acatgggcaa tgaatcgaca taatcagaaa aagaagtttc
    10381 aacatccttt tgaccaatgg aaatggcatg tccgagtgga cccaacgcat aataatcatt
    10441 ccacttagga tcaggctgag gcggcattac aattccatta aaaggatatg tattctcagg
    10501 aaaattatat ggataattaa atggttcttc ataaatgtca ttcagataat ctgctgggaa
    10561 ataaccttca ggtacatata gatcgagatg tggcattgcg aaacccaatg cgagaaaaaa
    10621 tactgaatcg cacaatgcaa tttcacctga ggctacatca ttaataaatt ctattgcaga
    10681 gtcaacagca cgataatcct ttaattcata ttctactgcg ctgtcaacaa caccccacag
    10741 gtaaccatct acgccaacat ttgtagcttc cagtttatcg gttgtactat cccaattgga
    10801 aaacgagatc accttgctcc atgataatgt ttttaaacat ggatcttctg tatccacatc
    10861 ataatcttta ttgaatggat catgaaatat tttattaata ccgcaggtat aatattgacc
    10921 cgaatttttt aaatattcag ggatagtgat aacttcttca ttacccagct ctgtagtgaa
    10981 attatctcta aattcatcga ggtattcgtt attatcatat acctgtgtat atttcatatc
    11041 cttaccactc aacatgcttg tccgagaggg accacataca ggagcattac aataggcatt
    11101 catgaaatta gttcccaggc caaacaagga atccataaca ggcgttttaa tctgaggctg
    11161 ccctccaaga gtgcctgtaa aatcattcat atcatctaaa atgataaaaa ctatattggg
    11221 tttttcaggc gtctgtgcat gcatgctcaa ggaacataca acaatagaaa gaaatgccaa
    11281 taataaacgg tgtgccttca tcataggtca ttgtacaatt atctgataag caaattctgt
    11341 agtcttagct acatataatc ccggcgacca ggttgctaca ttaatttcca tatccaccgt
    11401 caatttcgct tcaaatactt ttgcgcctgt aattgagtaa atttcaagat tctcattact
    11461 tggatttccg caatgcacat acaataagct tgaagtcgga tttggtaaaa gggtgaaata
    11521 attgtttgta aaattattca cttcaatata gtctgttttc acctcataat aataaacatc
    11581 atgctctgca ccttccgggt tgtaactttt ggtaaagaca tcgtcatcgg ctgtcgcttc
    11641 cacataaaaa cgtacttttg ttccaacagg aaagttggga atagcagatg caaaaatcaa
    11701 atcccccgct agttggtcgc catggagtcc atcatcatac ataatcaatt tggtaaatgg
    11761 tccgaaaatc tctgttgaat aatataaagt tatttgtttc acatcaggcg caccggatat
    11821 ttgagctgat attgtgacgg gttgaatact atttacaacg ccccaattat agccattgct
    11881 gatccacttt acattctcaa tatttaatga tggattgtca aattcattat cgtctaagat
    11941 ataggctctt cgatttgcaa tagtttcttt caattggtca ataccatata aaaattgatc
    12001 atagctaacc aatttgaccg ggtcagtcgc cacgtacgga tcaattaatt caaaatagtc
    12061 atcaataatt ccatacaaat tttcttcagt ataaatttca ttgaacatag ttctaaaatg
    12121 ggcaagatac cgttgtctga ttgaaggaat ggcaaagaaa tgatagctta aggcataatt
    12181 agtatctgtt cctttataaa atattttcca tttgtagcgc atagaatcca ttacactgtt
    12241 gccgtcatat tcaagcggca cgagacgatc tgtttcaggc tcccaatata aaaaatagtc
    12301 catgccccct ttatgcacat aactatcttc atctgtaaat agtatttccg ctgccatata
    12361 ccaaagtgtt cggtcaaggt cgagatattt gtttatagtg tcttccaaaa catcagcagg
    12421 catattttcg agtggatcgc aaacatcaat aatatcctcc catggatggg atatactcgt
    12481 ttctttcagg gtataataat ttttatagac acttgtatcc attccaaggt aattcaaagt
    12541 cgaaaatcct gttccattag cacctccggg gtctgcatct gtagctccat ctgtgcgttc
    12601 agccctccag agtgagccat cattggtgat aaaccaatct tttaaataaa ttttatcaag
    12661 ttgctgaacg ttagtgtaaa gtccaaaatc caaatcattt aaataaagat ggacataatt
    12721 taccgataaa gcaggtatgt atttttgtat caggttctca taaatagcct cgtgcatgaa
    12781 agtagtatct ctgaaactac aattcaaatt aagtgtttta gaaccatcaa tattctgaag
    12841 tgaatctata taatctaatg ttataccaac tgatttttta atggaatcat ttgcaaaaaa
    12901 gctcgaatga ccgcggaaat aaactccaac atcagcaggt aaggtatctc cgttataaac
    12961 caatctggca ggaatagcca gttctgtgcc gtgggtgtct aataattgat cataccagtc
    13021 atcaacataa aaaagaagat taatctcacg tatcacatta atatcatata aatctgaagg
    13081 aggattacca ccaacggtca tatgatgggt tccttcaaaa taatttaatt gatacggtaa
    13141 ggtttgtgcg aagccgttgg tagcttgaat aacaaacaac atacaaacaa aacatttgag
    13201 ggatttcgac atgggaatga gctttttcaa aaagtgtaaa tattgaatgg taataataac
    13261 ttaatgtaaa attatcattc aattttaaat tctcaaccgg aattgagcga agcgacctgt
    13321 agagcttgtg aactggatgg gtaattgagt gaatgggtta ttaggaagag ctctgaacca
    13381 actgagctat ccgtttacga ctcttccagc ctttaacctc ttgttcacga tgataagcag
    13441 cggacttatc ttcaaatccc tccgtataca ctattttcca atcctttgcc ttaccggtga
    13501 acccttcatg gtcagacaag tgcctacgca agcgttcttg aacatcttca caagtatgtc
    13561 caatgtaata acgatctaat gtaatagaat gtaaaatgta gcagcaacac ataatcaaca
    13621 acactttatc tcaaaaaaaa aggtcgcaat gcgacctctt gttgtgggaa cacttgggct
    13681 cgaaccaagg accctcccgc cttaaagcgg gatgctccga accaactgag ctatccgttt
    13741 cttacttttc caacctttaa tttctcgttc tcgccgataa gcagcagact tatcttcaaa
    13801 tccctccgta tacactattt tccaatcctt tgccttaccg gtgaaccctt catggtcaga
    13861 caagtgctta cgcaagcgtt cttgaacatc ttcacaagta tgtccaatgt aataacggtc
    13921 taatgtaata gagtataaaa tgtagcagca acacataatc aacaacacca tttcctgtaa
    13981 aaagaaaaag accgcgattg cggtcttttg aagtgggaac acttgggctc gaaccaagga
    14041 ccctctgctt gtaaggcaga tgctctgaac caactgagct atgctcccaa tattttaaat
    14101 aactttctca cctggaccct cccgcattaa agcgggatgc tctgaaccaa ctgagctatg
    14161 ctcccaatat tttaaataac tttctcaatc ggaccctccc gcctttagcg ggatgctctg
    14221 aaccaactga gctatgctcc caata
//
LOCUS       DAOQHY010000083        14168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2053_length_14168_cov_5.534117, whole genome shotgun sequence.
ACCESSION   DAOQHY010000083 DAOQHY010000000
VERSION     DAOQHY010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 14168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14168
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2053_length_14168_cov_5.534117"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2985)
                     /gene="rpoB"
                     /locus_tag="PLL28_08225"
     CDS             complement(<1..2985)
                     /gene="rpoB"
                     /locus_tag="PLL28_08225"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNF69350.1"
                     /translation="MPKSTATIRRPERINFGKIKRADTYPDLLDIQVKSFKDFFQLET
                     TPENRRAEGLYRVFNENFPITDARNIFVLEFLDYFVDPPRYSMEECMERGLTYSVPLK
                     AKLRLSCNDEEHIDFQTIVQDVFLGNIPYMTPKGTFIINGAERIVVSQLHRSPGVFFG
                     QSIHPNGTKIYSARVIPFKGAWMEFATDINNVMYAYIDRKKKFPVTTLLRAIGYDTDK
                     DILELFGLAEEVKADDKTLRKHIGRKLAARVLKTWREDFVDEDTGEVVSIERNEVILE
                     RDTVLTDDNISLIIETGAAKVILTRDDVTVDYGIILNTLQKDVSNSELEAVQHIYRQL
                     RGAEPPDDETARGIIEKLFFSDKRYDLGEVGRYKINQKLNHHHIALDTKVLTKDDIIA
                     IIRYLVELSNNKAEIDDIDHLSNRRVRTVGEQLYAQFGVGLARMARTIRERMNVRDNE
                     VFTPIELINARTLSSVINSFFGTSQLSQFLDQVNPLAEITHKRRISALGPGGLSRERA
                     GFEVRDVHYSHYGRLCTIETPEGPNIGLISTLCVHAKINRMGFIETPYRKVDNGKVDM
                     KGDILYLNADEEDRFVIAQSNVPVDDKGKFAISKIKGRQQGEFMEFEPEQVEYMDVAP
                     NQIVGVSASLIPFLEHDDANRALMGSNMQRQAVPLLRPQSPVVGTGLEEKVASDSKML
                     INAEGNGVVEYVDANEVVIRYDRNEDDQMVSFEDSRKVYKLRKFKKTNQSTCINLKPI
                     VVKGQRVAVGQVLCEGYGTQGGELALGTNLKVAFMPWKGYNFEDAIVISERVVQEDIF
                     TSIQIEEFELEVRDTKLGEEELTSDIPNVSEEATKDLDANGIIRLGAHVKEGDILIGK
                     ITPKGETDPTPEEKLLRAIFGDKAGDVKDASLKVPPSIEGTVIDKKLFARAKKDKNAK
                     AREKIALEKLEKEHARALDELRTILIDKLSIILKDKKTPGLKSLYKEEMVPAKTKFNN
                     KVLEGINYANVDPYNWTGEADTD"
     gene            complement(3102..3488)
                     /gene="rplL"
                     /locus_tag="PLL28_08230"
     CDS             complement(3102..3488)
                     /gene="rplL"
                     /locus_tag="PLL28_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNF69351.1"
                     /translation="MAELKEFAEKLVSLSVKEVNELAKILKEEYGIEPAASAPVMMAA
                     AGGGAGAGADAAAEKTSFDVILKNAGAAKLSVVKVVKDLTGLGLKEAKDLVDGAPKSV
                     KEGVDKATAEDIAKQLQEAGAEVEIK"
     gene            complement(3545..4075)
                     /gene="rplJ"
                     /locus_tag="PLL28_08235"
     CDS             complement(3545..4075)
                     /gene="rplJ"
                     /locus_tag="PLL28_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNF69352.1"
                     /translation="MNKEQKTAVIEELKETVSKYDNFYLTDASAMTVEEVNKLRRMCF
                     ENGIRYEVVKNTLLRKAFEAHGVKYEGLFNLLHGETSVMFSESSNQPAKLIKKFRKEN
                     DKEKPLLKGAYVGADVFLGDNSLDALISLKSKEELIGEIIGLLQSPAKNVISALLSSE
                     QKLAGIVKTLSEKEEK"
     gene            complement(4100..4792)
                     /gene="rplA"
                     /locus_tag="PLL28_08240"
     CDS             complement(4100..4792)
                     /gene="rplA"
                     /locus_tag="PLL28_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNF69353.1"
                     /translation="MTKNRKKVQALVDSNKLYTLREAADLVKKSTTCKFNASVDLHVR
                     LGIDPRKADQAIRGTTALPHGTGRDKKVLVLCTPEKEDEARKAGADYVGLDEFIDKIV
                     NGWIDMDVVIATPSVMPKIAKLGRVLGPRNLMPNPKVGTVTNEVGDAITEVKRGKVAF
                     KVDKYGIIHSSIGRISFNTDQIAENSEELIRALAKLKPSSAKGAYFKSITMASTMSPG
                     IAIDPKSLADIN"
     gene            complement(4825..5271)
                     /gene="rplK"
                     /locus_tag="PLL28_08245"
     CDS             complement(4825..5271)
                     /gene="rplK"
                     /locus_tag="PLL28_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNF69354.1"
                     /translation="MAKEIQTYVKLQVKGGQANPAPPIGPALGSKGINIMEFCKAFNA
                     RTQDKMGKVLPVVITVYKDKSFDFIIKTPPAAILVLEAAKKQKGSAEPNRNKVGSITW
                     DQVKTIAQEKMPDLNCFTIESAMKMVAGTARSLGITVTGQAPWMNN"
     gene            complement(5273..5821)
                     /gene="nusG"
                     /locus_tag="PLL28_08250"
     CDS             complement(5273..5821)
                     /gene="nusG"
                     /locus_tag="PLL28_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNF69355.1"
                     /translation="MADDKKWYVLRVISGKERKLREYVEQHVKRLGWEHIISQVLVPT
                     EKVYKIKNGKKVIHEKNSLPGYILIEAVEGKMTPEMMQVITGITNVIDFLGKENPVAL
                     RRNEVNRILGKMDEMSEEGVSLNEPFIIGETVKIIDGPFNEFLGNVDEVHEDKKKVKV
                     IVKIFGRKTPVELNFAQVEKVN"
     gene            complement(5841..6032)
                     /gene="secE"
                     /locus_tag="PLL28_08255"
     CDS             complement(5841..6032)
                     /gene="secE"
                     /locus_tag="PLL28_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNF69356.1"
                     /translation="MNKITSFFSEAYQELRYKVSWPTWAELQSSTQLVLVAFLLISVV
                     VFVMDLASKELLDLFYGRF"
     gene            complement(6048..6120)
                     /locus_tag="PLL28_08260"
     tRNA            complement(6048..6120)
                     /locus_tag="PLL28_08260"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6085..6087),aa:Trp,seq:cca)
     gene            complement(6167..7354)
                     /gene="tuf"
                     /locus_tag="PLL28_08265"
     CDS             complement(6167..7354)
                     /gene="tuf"
                     /locus_tag="PLL28_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNF69357.1"
                     /translation="MAKETFVRSKPHVNIGTIGHVDHGKTTLTSAITYVLSEQGLAQK
                     KDYDSIDAAPEEKERGITINTAHVEYETTNRHYAHVDCPGHADYVKNMVTGAAQMDGA
                     ILVVAATDGPMPQTREHILLARQVGVPAIVVFMNKVDLVDDPELLDLVEMEVRDLLSF
                     YSFPGDTISIIKGSALKALEGDATGKQAIMDLMAAVDNDIPIPPRLVDQPFLMPVEDV
                     FSITGRGTVATGRIERGVINTGDPVDIIGLTAESLKSTVTGVEMFRKLLERGEAGDNA
                     GLLLRGVEKEQIKRGMVICKPGSITPHTEFKCEIYVLKKEEGGRHTPFFNKYRPQFYF
                     RTTDVTGEITLPDGVEMVMPGDNIAMTVKLIYPIAMEKNLRFAIREGGRTVGAGQVTE
                     ILK"
     gene            complement(7466..7538)
                     /locus_tag="PLL28_08270"
     tRNA            complement(7466..7538)
                     /locus_tag="PLL28_08270"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7503..7505),aa:Thr,seq:ggt)
     gene            complement(7549..7624)
                     /locus_tag="PLL28_08275"
     tRNA            complement(7549..7624)
                     /locus_tag="PLL28_08275"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7589..7591),aa:Gly,seq:tcc)
     gene            complement(7740..7822)
                     /locus_tag="PLL28_08280"
     tRNA            complement(7740..7822)
                     /locus_tag="PLL28_08280"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7786..7788),aa:Tyr,seq:gta)
     gene            complement(7832..7904)
                     /locus_tag="PLL28_08285"
     tRNA            complement(7832..7904)
                     /locus_tag="PLL28_08285"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7869..7871),aa:Thr,seq:tgt)
     gene            complement(8146..8445)
                     /locus_tag="PLL28_08290"
     CDS             complement(8146..8445)
                     /locus_tag="PLL28_08290"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HNF69358.1"
                     /translation="MEIKIQSIRFDADKQLLEQIRTKVEKLQKFDASLAEAQVYLKLD
                     AELGGFHEKVVEIKLTKPGNTYFSKAERGTFEDGLDEVLEALEVQVKKQRDKEKS"
     gene            complement(8478..>8773)
                     /locus_tag="PLL28_08295"
     CDS             complement(8478..>8773)
                     /locus_tag="PLL28_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNF69359.1"
                     /translation="RLYGKGEHFLITGAGKPAYPKMVYNIVHNVLQQVTTLTRTSPHV
                     LRHTFATHLSNNGAELNAVKELLGHASLASTQVYTHNTIDQLKKIHKRAHPKG"
     assembly_gap    8774..8873
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8874..9157)
                     /locus_tag="PLL28_08300"
     CDS             complement(<8874..9157)
                     /locus_tag="PLL28_08300"
                     /inference="COORDINATES: protein motif:HMM:NF014898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNF69360.1"
                     /translation="MHLEGFYTYLQYERRYSKHTLAAYRNDISQFHDFLVKEYGSEDP
                     DMLDVHHRQVRAWIIALMEDKTAARSVNRKISALKTYYRYCQQQKAINHNP"
     gene            complement(9210..9407)
                     /gene="rpsU"
                     /locus_tag="PLL28_08305"
     CDS             complement(9210..9407)
                     /gene="rpsU"
                     /locus_tag="PLL28_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNF69361.1"
                     /translation="MLIIDAREAESIDKALKTYKKKFEKAGIVKELRRRQSFTKPSIE
                     RRQEIIKARYKQTFVTEANQS"
     gene            complement(9465..10604)
                     /locus_tag="PLL28_08310"
     CDS             complement(9465..10604)
                     /locus_tag="PLL28_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNF69362.1"
                     /translation="MSHTISHDISIITDTAKEFAEKYVRPHVMDWDESQHFPIEIFHK
                     AGELGLMGVLVPEQYGGSGLGYIEYMRIIEEIAAVCGSIGLSVAAHNSLCTGHILQFG
                     NESQKSKYLPLLASGQWIGAWGLTEPNTGSDAGNMQCVATREGDEWVLNGTKCWITHG
                     KSSSVMVVIARTGAPRTPNNCTAFVVERGTPGFSAGKKENKLGMRASETTEVIFDGCR
                     ISDAQRLGEVGEGFKQAMKVLDGGRISIAALSLGIAKGAYRAALKYAKERHQFDQPIA
                     NFQAISFKLADMATRIEAADLLTLKAADLKNKGEKVTEISAKAKYYASEVSVAVATDA
                     VQIFGGYGYTKDFPVEKYYRDSKLCTIGEGTSEVQKLVIAREILR"
     gene            complement(10640..11170)
                     /locus_tag="PLL28_08315"
     CDS             complement(10640..11170)
                     /locus_tag="PLL28_08315"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002634.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNF69363.1"
                     /translation="MSIEQELKQAIRDVADFPKEGIVFKDITPLLSDPVLSDKIGDAL
                     LDRVKDLKPDAIVAIESRGFLYGFLLANKLQIPFIPVRKKGKLPGDTLEYTYDLEYGS
                     ATVEIHTMDVKPGWRVLVHDDLLATGGTAQAAAELILMQGAHVAGFAFVVELSFLGGV
                     KKLNHYTDNIVSLATY"
     gene            complement(11167..12033)
                     /locus_tag="PLL28_08320"
     CDS             complement(11167..12033)
                     /locus_tag="PLL28_08320"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNF69364.1"
                     /translation="MLLILPDILHALIKKPDTKDNYALWQSLKMRTDTTSIYQSDHSF
                     QDSNSYAENYNYPFQRYKTEQQNVELSFITPSKPFNPNMISAEEWQSFGVPKYVAERI
                     IKYRQKSGTFKSSTDVKKIYGFPDDVFATIEPFMIFQDSSAVTVADHSGGDLQIVLTS
                     TNLNTCTKEDLLSLGFSPNDATRILRFREQAGGIYDSQQLFGVYGIDADKLQELIKWL
                     EVDKNSVIKINLNTIDSTTLANHIYISDILAGSIIRYREATGKFYSVNELLKVKGMYP
                     ALFEKLKPYLQL"
     gene            complement(12187..12330)
                     /locus_tag="PLL28_08325"
     CDS             complement(12187..12330)
                     /locus_tag="PLL28_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69365.1"
                     /translation="MSSSALIMMIATVSVVTLVTAYFFYLVLKTPPREEPDSFSENDD
                     VKR"
     gene            complement(12327..14009)
                     /locus_tag="PLL28_08330"
     CDS             complement(12327..14009)
                     /locus_tag="PLL28_08330"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HNF69366.1"
                     /translation="MTQKTESWGSRVGLILAMAGNAVGLGNFLRFPVQAIQNGGGAFI
                     IPYLVCFLLMGIPLLWIEWATGRFGGSKGHHSTPFILDSMDKRKFWKYIGVFGIFTNL
                     AVAAYYCYLESWTLNYVWNSISGTFRGESAESVSAYFNHYVNAGDAHQHWTSWIADNP
                     APIIFWIICLLLNTWILSKGLSGGVEKVAKIGMPLLIVFGVFLAIMAVSLRAGQHGAI
                     NGGEVGLNFLWTPNFDTIWNPKVWLAAAGQIFFTLSVGMGSIQCYASYVRKKDDIALN
                     AMSAGWMNEFVEVVLGAAIIIPISVGYLGIDKVVEMTQSGGLGLGFRTLPYLFEQWGP
                     TLAVICGVFWFGLLFFAGITSSLAMGTPWMGFMTDEFGWSRQKGAWSFGALVLILGLP
                     TVLFFNQGVFDEYDYWAGTVSLVVFALGETILFAWVFGMTKGWQEINSGGDIRVPSVY
                     KFVIKWVTPLLLLFVFLGALITPQGNDWVNAFNNGWVLDNSSIIKQITNAGLKEQMHA
                     ATTAGDTATMDALHDKLLYINIARILLVSVFLGVAWLVYTAYRKRIKEGRFI"
BASE COUNT         3677 a         3253 c         2878 g         4260 t
ORIGIN      
        1 atcagtatct gcttcaccgg tccaattgta aggatctacg ttggcatagt taataccttc
       61 gagaactttg ttgttgaatt tggtttttgc aggtaccatt tcttccttat aaagcgactt
      121 cagacccgga gtctttttgt ctttcaaaat gatagacaat ttgtcgataa ggatagtacg
      181 caattcatct aaagcacgtg catgttcttt ctcaagtttc tcgagagcga ttttttcgcg
      241 cgcttttgca tttttatctt tcttggcacg tgcaaacaat ttcttgtcga tcacggtacc
      301 ctcgattgat ggtggtactt tcaatgaagc atctttcaca tcaccggctt tatcgccgaa
      361 gatggcacga agaagttttt cttcaggagt aggatctgtt tcacccttag gtgtaatctt
      421 accgatgaga atatctcctt cttttacatg tgctccgagg cgaatgatac cgtttgcatc
      481 gaggtctttt gtagcctctt cagaaacgtt tggaatatca gaggtcaatt cctcttcacc
      541 caatttcgta tcacgcactt caagttcgaa ttcttctatt tggatggaag tgaagatatc
      601 ttcctgcaca acgcgctcgc tgatcacgat cgcatcttca aagttgtaac ctttccaagg
      661 catgaatgcc actttcaagt tagttccgag tgcaagctca ccgccttgtg taccataacc
      721 ttcgcataat acctgaccaa ctgctacgcg ctgacctttc accacgattg gtttaaggtt
      781 aatacatgtg ctttggttgg tctttttgaa tttgcggagt ttatatactt tgcggctgtc
      841 ttcaaagctc accatctgat catcttcatt gcgatcgtat cggatcacaa cttcatttgc
      901 atcaacatat tcaacaacac cattaccttc tgcgttgatg agcatttttg agtcagatgc
      961 tactttttct tccaaaccgg ttccaactac aggcgactgc ggacgaagca gcggaactgc
     1021 ctgacgttgc atgtttgaac ccatgagcgc gcggttcgca tcgtcgtgct cgaggaaagg
     1081 aatcaatgat gcagatacac caacaatttg gtttggtgcc acatccatat attctacctg
     1141 ttcaggttca aattccatga attcaccctg ctgacgacct ttgattttgc tgatggcaaa
     1201 ttttccttta tcatcaacag gcacgttaga ctgagcgatc acaaaacggt cttcttcgtc
     1261 ggcattcaaa tacagaatat cgcctttcat atctacttta ccattatcta ctttgcgata
     1321 aggtgtttcg ataaagccca ttctgttaat cttggcatgc acacaaagtg tagagataag
     1381 accgatgttt ggtccttccg gtgtttcgat agtacacaaa cggccatagt gactgtagtg
     1441 tacgtcgcgc acctcgaaac ctgcacgttc gcggctcaat ccgccgggac cgagagcgga
     1501 gatacgacgt ttatgcgtaa tctctgcaag agggtttacc tggtcgagga actggctgag
     1561 ctgagaggtt ccgaagaacg agttgatcac tgaagaaagt gttcttgcgt tgatcaattc
     1621 aatcggtgta aatacctcat tatcgcgcac gttcatacgc tcgcggattg ttcttgccat
     1681 acgagcaaga ccaacaccga actgcgcata caactgttca cccacggtac gcacgcgacg
     1741 gttgctcaag tgatcgatat catcaatctc ggccttgtta tttgataatt caacaaggta
     1801 tcggatgatg gcaatgatat catctttagt gagcactttg gtgtcaagtg caatatggtg
     1861 gtgattcagt ttttggttga tcttataacg accaacttct ccgaggtcgt aacgcttgtc
     1921 ggagaagaac aatttctcga taataccacg tgctgtttca tcatcaggtg gttctgcacc
     1981 acgaagctga cgatagatat gttgcaccgc ttccagttca gagttggaaa cgtctttttg
     2041 aagggtatta aggatgatac cgtagtcgac agttacgtcg tcgcgggtaa ggatcacttt
     2101 agcggcacct gtttcaatga tgaggctgat attatcatca gtgagaacgg tatcgcgctc
     2161 aaggattact tcattacgtt cgatagacac cacttcaccg gtatcttcat ccacaaaatc
     2221 ctcgcgccat gttttgagaa cgcgggcagc gagttttcgt ccgatgtgtt tacggagtgt
     2281 cttgtcatcg gctttcactt cttcagcaag tccgaagagt tccaggatat ccttatcggt
     2341 atcatatccg atggcgcgta ataatgttgt taccgggaat ttcttcttac ggtcgatgta
     2401 ggcgtacatc acgttattaa tatcggtagc gaattccatc cacgcgcctt taaaaggaat
     2461 tacgcgggcg gaatagatct tagttccatt cgggtgaata gactgaccga agaatacacc
     2521 gggagagcgg tgtaactgtg aaaccacgat acgttcggca ccattaataa tgaaggtacc
     2581 tttcggggtc atatacggga tattacccaa gaatacgtct tgaacgatgg tttggaaatc
     2641 gatatgttcc tcatcgttac atgacaacct gagtttagcc ttcagtggaa cggagtacgt
     2701 aagtccgcgc tccatacact cttccatcga ataacgtgga ggatccacga aataatcgag
     2761 aaattccaat acaaagatgt ttcgcgcatc agtgatggga aagttctcgt tgaacacgcg
     2821 atacaggcct tcagccctcc tgttttcagg cgtggtctcg agttggaaaa agtctttgaa
     2881 ggatttaacc tgtatgtcta ataggtctgg ataagtatct gctcttttaa tctttccgaa
     2941 gttgattctt tctggtctcc tgatggttgc agtagatttt ggcatacggt atcaaagctt
     3001 tattgcatta aaataataaa caggctaacc gccgtacggc ggattagcct tgtattttaa
     3061 ccacgccgta cggcgcgagg aagttaattg ccaaggagag attacttgat ctctacttcg
     3121 gcacctgctt cctgaagttg cttagcaata tcttcagcag ttgctttatc tactccttct
     3181 ttcacagatt taggagcgcc gtcaacgagg tctttcgctt ctttaagtcc gaggcctgtg
     3241 aggtctttta ctactttcac aactgaaagt ttagcagcac cagcgttttt caggatcaca
     3301 tcaaaagatg ttttctctgc agcagcgtca gcaccggcac cggcaccacc gcctgcagca
     3361 gccatcatta cgggagcaga agcagctggt tcgataccat actcttcttt caggattttt
     3421 gccagttcat taacctcttt tacagataag ctaaccaact tctctgcaaa ttcttttaat
     3481 tcagccattg ctttgagttt ttattgattt agaaaattga tttaaataat agttgttgtg
     3541 ttaattattt ttcttccttt tcggaaagtg tttttacgat tccggccaat ttctgttcgc
     3601 ttgacaacaa agcagaaata acatttttgg caggtgactg gagcaagccg atgatctcgc
     3661 caatgagttc ttctttgctc ttcaggctga tgagtgcatc gaggctgtta tcgccaagga
     3721 atacatctgc accaacataa gcgcccttca acaatggttt ttctttgtcg ttctctttgc
     3781 ggaatttctt gatcagtttt gcaggctgat tagaagattc gctgaacatt accgatgtct
     3841 caccatgcag taagttaaag agaccttcat attttacacc atgtgcttcg aaggctttgc
     3901 gcaggagtgt gttttttact acttcataac ggataccgtt ttcgaagcac attctgcgca
     3961 gtttatttac ctcttcaacg gtcattgctg aagcgtcggt caggtagaag ttatcatact
     4021 tcgataccgt ttccttcagt tcttcaatga cggctgtttt ttgttcctta ttcatctttt
     4081 tctgatttcg atgattacat taattaatgt cagctaaact tttagggtcg atagcgattc
     4141 ccgggctcat tgtgcttgcc atggtgatgc ttttaaagta agcacctttt gctgaagatg
     4201 gtttcagttt ggcgagcgca cgaatcagtt cttcgctatt ttctgcgatt tgatcggtat
     4261 tgaagctgat gcgaccgata gatgaatgga tgataccata tttatctacc ttaaatgcca
     4321 ctttacctct tttcacttct gtgatagcat cacccacttc gttggttaca gtaccaactt
     4381 tagggtttgg catcaagttg cgtggaccga gtacgcgacc cagttttgca attttaggca
     4441 tcacgcttgg agtggcgata accacatcca tgtcgatcca gccatttacg atcttatcga
     4501 tgaattcatc gagacctaca tagtctgcac ctgctttacg agcttcatct tccttttcag
     4561 gagtacagag cacgagcact tttttatctc ttcccgtacc gtgtgggagg gctgtagttc
     4621 cgcggattgc ctgatcggct ttacgtggat cgatacccaa gcgcacatgg aggtcaacag
     4681 atgcattgaa cttgcaagtt gtagattttt taacaaggtc agcagcttcc ctcaacgtat
     4741 atagtttgtt ggagtcaact aatgcctgaa ccttttttct gttctttgtc agtttcacgt
     4801 gtaaacggtt ttttagttga ctaattaatt attcatccag ggtgcctgac cggtaacggt
     4861 gatacctaaa ctgcgggcag ttccggctac cattttcata gcactttcga tggtgaaaca
     4921 attcaggtcg ggcatttttt cctgcgcgat agtttttacc tgatcccagg taatgctacc
     4981 aaccttatta cggtttggtt cagcagagcc cttttgtttt tttgctgctt ccagcacaag
     5041 gatggccgca ggtggtgttt taatgataaa gtcgaacgac ttgtctttat acaccgtgat
     5101 cactacagga agaacttttc ccattttatc ctgagttctt gcgttgaacg ctttacagaa
     5161 ctccatgata ttgatccctt tggaacccag tgcaggaccg attggaggtg ccgggtttgc
     5221 ctggccgcct tttacctgca gctttacgta cgtttgaatt tcttttgcca tatcagttta
     5281 ctttttccac ctgggcgaag tttagctcca ccggggtttt tctaccgaat atttttacaa
     5341 tcactttaac tttcttcttg tcttcgtgca cctcatcaac atttccaagg aattcgttga
     5401 aaggtccgtc gatgattttc acagtttctc cgataatgaa tggttcgttc aggcttacac
     5461 cttcttcgct catttcatcc attttaccca agatcctgtt cacctcatta cggcggagtg
     5521 ctaccgggtt ttctttacca aggaaatcga tcacattggt aataccggtg atcacttgca
     5581 tcatttcagg tgtcattttc ccttcgacag cttcgatcag gatataaccg ggaaggctgt
     5641 tcttttcatg aatcaccttc ttgccattct tgatcttata cactttttcc gtcggtacca
     5701 gcacctgaga aataatatgc tcccagccca gtcgctttac gtgttgctca acgtattcac
     5761 gcaatttgcg ctctttaccg ctgatcacgc gcaatacata ccacttttta tcgtctgcca
     5821 ttggtgctga ttaggttcga ttaaaatctt ccatagaaca ggtcgagcaa ctccttagag
     5881 gccagatcca ttacaaaaac cacaacagat atgagcagga atgctacgag taccagctgt
     5941 gtactggatt gtaactcagc ccaagtaggc caactcacct tgtagcgcag ttcctgatac
     6001 gcttcgctga aaaatgatgt aatcttgttc atattctttc cttgatgcac gggtggagag
     6061 gctcgaactc ccagcctgcg gttttggaga ccgccactct accaattgag ctacacccgt
     6121 ttgggaaaaa aggacccacc ggagcggatc cttttttgtt cgtttattat ttcaaaattt
     6181 cagttacctg acctgcacct acggtacgac caccttcgcg gatagcgaaa cggaggtttt
     6241 tctccatagc gatagggtaa atcagtttaa cagtcattgc aatgttatca ccgggcataa
     6301 ccatttcaac accatccggt agggtgattt cacctgttac gtcagttgtg cggaaataga
     6361 attgcggacg atatttattg aagaatggtg tgtgacgacc gccttcttct tttttcaata
     6421 cgtagatctc gcatttgaat tctgtatgcg gagtaattga acccggctta cagataacca
     6481 taccacgttt gatttgttct ttctcaacac cacggaggag gagacctgcg ttatcgccgg
     6541 cttcaccacg ctcgaggagt ttgcggaaca tctcaacacc tgttacggta gatttcaatg
     6601 actcagcagt caaaccgatg atatctacag gatcacctgt attgataaca ccacgctcga
     6661 tacggcctgt agcaacggta ccacgaccgg tgatagagaa cacgtcttca accggcataa
     6721 ggaacggttg atcaaccagc cttggaggaa ttggaatgtc attgtcaact gcagccatca
     6781 aatccatgat cgcttgtttt cctgttgcat ctccttcgag agctttcaat gcagaacctt
     6841 tgatgataga gatagtatcg cctgggaagc tgtagaagct taacagatcg cgaacttcca
     6901 tctcaacaag atccaacaat tcaggatcgt caacgaggtc aactttgttc atgaacacaa
     6961 cgatcgcagg tacacctacc tgacgagcaa gaaggatgtg ctcacgagtt tgaggcatag
     7021 gtccatcagt tgcagcaacc acgagaatcg cgccgtccat ttgggcagca ccagtaacca
     7081 tgttctttac gtagtcggcg tgtcccggac agtccacgtg cgcatagtga cggttagtag
     7141 tttcgtattc aacgtgggca gtgttgatgg tgatacccct ttctttttct tcaggagcag
     7201 cgtcgattga atcgtagtct tttttctgag caagaccttg ttcagaaaga acatacgtga
     7261 tcgctgaagt aagagtggtc ttaccgtgat ccacgtgacc gatagtaccg atgttcacgt
     7321 gtggtttgga acgtacaaat gtttcttttg ccatttttat tcgatttaga acttgttgta
     7381 tagtaattgt tattaaacct ctactttctt ttgccctctc tgcagtattg ctacttcaga
     7441 taacaccggg caggtgcttt ttgtgagcca atggccagga tcgaactggc gacctcatcc
     7501 ttaccatgga tgtgctctac caactgagct acattggctg ataaccggtg gagcgggaga
     7561 cgagactcga acccgcgaca ttcagcttgg aaggctgacg ctctaccaac tgagctactc
     7621 ccgcttttaa tacattctta ctgagaaaac gacgaagaca ccgatgcacg agagcagaac
     7681 gcccccgaaa catttttgtt ttcggcgtgg tttctcagtt tcaaagaact gatggatagt
     7741 gggaagaggt ggattcgaac caccgaagtc gtaagacaac agatttacag tctgccccat
     7801 ttggccactc tggtatcttc cctccgccaa gagccaatgg acggactcga accgccgacc
     7861 ggctgattac aaatcagcag ctctaccaac tgagctacat tggcttgaca aaatctcttt
     7921 tctgctctgt tgtggtgccg aagtaatgcc gcaacccgcc aatgtcattt ctgacaccca
     7981 aaaccattgg tttcagaccg ggcctcactg acctattacc tatgttatta ttatcaaaga
     8041 aacgttagat ttttcctcac tttttggaaa aacgcccgca aatatacgaa ctttttgttg
     8101 atatgtgcaa aagctgcaag aatttggaac gattttttct tgcaatcagc ttttttcttt
     8161 atcccgctgc tttttaacct gcacctccaa tgcctccagc acttcatcaa gcccatcttc
     8221 aaaggttcct cgttcggctt ttgaaaaata ggtattaccc ggctttgtaa gtttgatctc
     8281 aactactttt tcatggaatc cgcctaattc tgcgtccagt ttcaagtaca cctgagcctc
     8341 agctaaagaa gcatcaaact tttgcagttt ctccactttt gtcctgatct gttccaacaa
     8401 ttgtttgtcg gcgtcaaatc ttatcgattg tatcttgatc tccattgctt tatgtttggg
     8461 gtggcgaaaa tatgcactta tcccttagga tgagcacgtt tatgtatttt ttttaactga
     8521 tctatagtat tatgggtata cacttgcgtg ctggcaagac ttgcgtggcc caataactcc
     8581 ttcactgcat tcaactcggc gccattattg cttaaatggg tggcgaaagt gtgacgcagc
     8641 acatgcggac tggttcttgt cagggttgtt acctgctgca atacgttatg tacgatatta
     8701 tataccatct taggatatgc cggttttcct gctccggtta tcagaaaatg ttccccttta
     8761 ccatatagac gatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggattgt
     8881 gattgatggc cttttgctgc tggcaatagc ggtaataagt tttaagtgct gaaattttgc
     8941 ggttgacact gcgggcagcc gttttgtctt ccatcaaagc aatgatccat gccctcacct
     9001 gtcgatgatg cacatcaagc atatcaggat cttcagaccc gtattcctta actagaaaat
     9061 cgtggaattg cgaaatatcg ttgcgatagg cagccagcgt atgcttgctg tatcgtcgtt
     9121 cgtattgtaa gtaagtataa aagccttcca ggtgcatatg ttcatgcagc gctaatgact
     9181 aagcaccgct atcggcgcac cgaaagggat cagctctgat tagcctcagt tacaaatgtt
     9241 tgtttgtaac gagccttgat gatctcttgt ctgcgttcga tagacggttt ggtaaaagat
     9301 tgacgtctgc gcaattcttt tactataccg gccttttcaa acttcttttt gtaggttttg
     9361 agggccttgt ctattgattc tgcttctctt gcgtcgataa tgagcattct gatattattt
     9421 gggagtgcaa aagtaataaa aattaatttc tcaaacaagt atcatcaacg aagtatctcg
     9481 cgggcgatta ccaatttctg aacctctgag gtgccttcgc caatggtaca aagctttgag
     9541 tcgcggtaat atttttctac cggaaagtct ttggtatagc catatccgcc aaaaatctgc
     9601 actgcatcag tagcaacagc aacacttact tcagatgcgt agtattttgc ttttgcagaa
     9661 atttcggtca ctttttcgcc tttgttttta aggtcggcag ccttcaatgt taacaaatcg
     9721 gctgcttcaa tccgtgtagc catatctgcc aatttgaagc tgatagcctg aaagtttgca
     9781 atcggctggt cgaactggtg acgttcttta gcatatttca atgctgcccg ataagctcct
     9841 ttcgcaatac cgaggctaag tgccgcaatg gagattcttc cgccgtcaag cactttcatc
     9901 gcctgtttaa agccctctcc tacttcacct aatcgctgtg catcgctgat acggcagccg
     9961 tcaaagatca cttctgtggt ctcgctagcg cgcatgccga gtttattttc cttttttcct
    10021 gcagaaaatc cgggggttcc tcgctctaca acgaaagccg tacaattatt aggtgttcta
    10081 ggagcaccgg tacgagcaat cactaccatt acgctgcttg atttgccatg cgtaatccag
    10141 cattttgtgc cgttcaacac ccattcatct ccctcccggg ttgccacaca ttgcatattt
    10201 ccggcatcac taccggtgtt aggctctgta agtccccatg caccaatcca ctgccctgaa
    10261 gcgagcaatg gcaaatattt cgatttttga gattcgttgc cgaattgtaa aatatgtcct
    10321 gtacaaagag aattatgcgc agccacgctc aagccgattg aaccacatac tgcggcaatc
    10381 tcttcgatga tacgcatata ttcaatatat cccaatcctg atccaccgta ttgttcagga
    10441 actaatacac ccatcaaacc aagttcaccg gctttatgaa aaatttcgat ggggaaatgc
    10501 tgactttcgt cccaatccat aacatgtgga cggacatatt tttcagcaaa ttctttcgct
    10561 gtatcagtta taatggaaat atcgtgagag atcgtatgag acatagcctt ttcgtgtttt
    10621 taaaacgacg acaaaggtat taatatgtcg ctaacgatac aatattatct gtatagtgat
    10681 tcaatttctt aacaccaccg agaaatgaca attcaacaac aaatgcaaat cctgcaacat
    10741 gtgcaccttg cattaaaatg agttctgctg cagcttgtgc ggtgccgccg gtggctaata
    10801 aatcatcatg cactaataca cgccatccgg gtttaacatc catggtgtga atttcaacgg
    10861 tggcgcttcc gtattccaaa tcgtaggtat attctaaggt atctccggga agtttacctt
    10921 tttttcgcac aggaatgaat ggaatttgca atttatttgc cagtaaaaat ccataaagaa
    10981 aacctcgact ttcaatggct acaattgcgt cgggcttgag atcttttaca cgatcgagta
    11041 aggcatcgcc aattttatcg gataacaccg gatctgacag aagtggcgta atatctttaa
    11101 acacaattcc ttctttcgga aaatcggcga catctctgat ggcttgtttt aattcttgct
    11161 cgatactcat agctggaggt atggttttaa tttttcaaac agcgcaggat acatgccttt
    11221 tactttcaag agctcgttta cagaataaaa cttgcccgtt gcttccctgt atcggataat
    11281 gcttcctgct aagatgtctg aaatatagat atggtttgcg agagttgtac tatcaattgt
    11341 gttgagattg atttttatca ccgaattttt atccacctcc agccatttta tcaattcctg
    11401 caatttgtcg gcgtcgatac catatacacc aaataattgt tgcgaatcgt aaatgcctcc
    11461 tgcttgctcg cgaaagcgca ggatacgtgt tgcatcgttg ggactaaagc ccagggatag
    11521 caaatcttct ttcgtgcaag tgtttaaatt cgtggatgtt aaaactattt gcaaatcacc
    11581 tccagaatga tcagcaaccg ttactgcaga tgaatcctga aatatcataa acggctctat
    11641 ggttgcaaag acatcatccg ggaatccata aatttttttc acatctgtac tacttttaaa
    11701 tgtgccgctc ttctgcctgt atttaattat tctttcagca acatatttag gcactccgaa
    11761 cgattgccat tcttctgcag aaatcatatt cggattaaag ggtttggatg gagttataaa
    11821 gcttaattca acattttgtt gttcagtttt atacctctga aatggataat tataattttc
    11881 tgcataacta ttactatcct gaaaggaatg atcactttgg taaatcgatg tagtatctgt
    11941 tcgcattttc agcgactgcc acaatgcata gttatccttt gtatcaggtt tcttaattaa
    12001 tgcatgtaag atatcaggta atatcaatag catgagcaca aggcaaagca atagccatgc
    12061 tgcgcgcctt tctcttctag agaaaataaa ataattctgt aattgttttc gcatagtaaa
    12121 cctccacaac acctatccct acagattgga aaaccatgca taaaacaaac aatgagagcg
    12181 aaaatattat cgcttcacat cgtcgttttc agaaaacgaa tccggttctt cacgcggtgg
    12241 cgttttcagc accagataaa agaaatatgc tgttacaagt gtcactaccg aaacggtagc
    12301 aatcatcata attaatgctg agctactcat atgaatctac cttcttttat gcgtttacga
    12361 taagcagtat aaaccaacca ggctacacct aaaaacacgg atacaagtaa aatacgtgct
    12421 atattaatat acaacaattt atcatgcaat gcatccatag tagctgtatc ccctgcagtt
    12481 gttgccgcat gcatttgctc ttttaatcct gcattggtaa tttgttttat aatcgagcta
    12541 ttatccagca cccaaccatt gttaaatgca tttacccaat cgtttccctg aggcgttatc
    12601 aaagcaccca gaaaaacaaa taataataat aacggcgtaa cccactttat tacaaacttg
    12661 taaacagaag gcactctgat atcgccacca ctattaattt cctgccaacc ttttgtcatg
    12721 ccgaaaaccc atgcaaataa tattgtttcg ccaagcgcaa atactactaa gctcaccgta
    12781 cctgcccaat agtcgtattc atcaaaaaca ccttgattga aaaacaatac tgttggcaat
    12841 cccagaatga gtaccagagc accgaagctc catgcgcctt tttgtcggct ccatccaaat
    12901 tcatctgtca taaaacccat ccaaggagtt cccattgcca atgatgaggt gataccggca
    12961 aagaataata atccaaacca aaatacacca caaattactg caagcgtagg tccccattgt
    13021 tcgaacaaat aaggcaaggt gcggaaaccc agaccaagac cgccggattg cgtcatctca
    13081 actactttat caatacccag gtagccaact gaaatcggaa taatgatagc agcaccgagc
    13141 actacttcaa caaattcgtt catccatcct gcactcattg cattaagtgc gatgtcatct
    13201 ttctttctta cataagatgc atagcattgt atcgacccca ttcccacaga tagggtaaag
    13261 aatatttgtc cggcagctgc caaccagact ttaggattcc atatcgtgtc aaaatttggt
    13321 gtccacaaga aattaagccc tacttcacct ccattaattg ccccatgctg acctgctctt
    13381 aaagaaacag ccatgatggc caggaataca ccgaacacga tgagtagcgg cattccaatt
    13441 tttgctactt tttccacacc accgcttaat cctttcgaaa ggatccaagt attcaacagc
    13501 agacaaatta tccaaaaaat aatcggagcc gggttatcag ctatccagga ggtccaatgt
    13561 tgatgggcat caccggcatt tacataatgg ttaaaatacg cactgacact ttctgcagac
    13621 tcccctctga aggttcctga aattgaattc caaacataat tgagcgtcca ggactcaagg
    13681 taacaataat atgctgctac tgccaggttg gtgaaaattc cgaaaacgcc gatatacttc
    13741 caaaatttgc gtttgtccat tgaatccaaa ataaatgggg ttgaatgatg gcctttgctg
    13801 ccaccaaaac gacctgttgc ccactctatc cataacaatg gaatacccat taacagaaaa
    13861 cataccaggt atggaatgat aaatgcacca ccaccattct ggatggcttg tacggggaag
    13921 cgcaggaaat tacctaagcc aacagcatta ccagccatag cgaggatcag acctactcgg
    13981 gagccccagg attcagtttt ttgcgtcata ctttttcttt gaacagagtt gaatgagagc
    14041 gcaaaataca aatttttact tttcaaccaa attgggtgag tgggtgaatt ggtgagtggt
    14101 gagtggtgag tggtgagttt acctcgccgg gcaggcgggt ggtgagttta cctcgccggg
    14161 caggcggg
//
LOCUS       DAOQHY010000084        13819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2142_length_13819_cov_5.480166, whole genome shotgun sequence.
ACCESSION   DAOQHY010000084 DAOQHY010000000
VERSION     DAOQHY010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13819
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2142_length_13819_cov_5.480166"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..2590
                     /locus_tag="PLL28_08335"
     CDS             152..2590
                     /locus_tag="PLL28_08335"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNF69367.1"
                     /translation="MKKPVFSLFSFVAMAICSTLSINAQNTTFNCYLPDQGGSIREHN
                     VNFISLDLDVKFAPEDGLVSGIARYAFTALEPTVDSVFLDGPGIRVQEISLDNNATKF
                     KMDSAGITVYFNPPLIREAVHNMAIIYDANPKKGIYFNGWNVASTDNPNDPTIIRKQI
                     WTQGQGVDNRFWIPSYDGVNDKLLTMMHITFDSTYTVVSNGNLKNVSANADGTKTWRY
                     AMPHPHALYLVMLAIGKYDVMEFTSKNGITTRQYYYPGTKEYAENTYRYTAELMDWMQ
                     QATGLKYPWSTYANVPVQEFLYGAMENTTATIFSDFFYQRPASNPDKQYIEINAHELT
                     HQWFGDYVTAWSGSSHWLQESFATYFSKHFAQHINGDDYYDWKRREEMLTALDADSRD
                     NIPIAHSEAGSQRVYQKGSFVIDMLRYVVGDQAFNVAIQEFLERYPYANVSTQDFQMQ
                     FMRSLGMNMDWFFDEWLYRAGFPVYTVKYDTTENATVVHVSQTQQTNETVHLFNMPIH
                     IQIHYMDGSFDDQLVVIKNAAQDITIQNPEHKQVLFVLFDPNHMVLSKVVFDKSFNEL
                     TYQAFNAPNMIDRYEAISAMTETPIEVKRVTLLALLKKEKFNNIRIEIIRQLSSDNDK
                     ATVAALKNAIYDTDPLVRRSVVVNWNKDNDKLRKDIETLLKDDNYVNIELALRKLCDY
                     YPKQTSEYLQKTAGQFGATNNIRVAWLELQNKDNNNGTMSELIKFTSPQYEFRTRVAA
                     FNAISNRNYCDPELVANLFNALVSTNHRLSSPARNLLMAYKSNPEYATMIKAYYNSRL
                     WTEWEDTRIGKVKE"
     gene            complement(2594..4744)
                     /locus_tag="PLL28_08340"
     CDS             complement(2594..4744)
                     /locus_tag="PLL28_08340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69368.1"
                     /translation="MKAVSFFVLAICLTVKFLYSQAPGILWQNTLQGSKSDLPYSVVN
                     AADGAYFISGISNSESSPDKSENNKGEFDYYDYWVVKMSNSGYVLWENTIGGKRDETN
                     SVVCSTPDGGCIVGGTSQSTATGDKTETSIGGEGDVDFWVLKLDASGNISWQNTIGGT
                     GSDQMRNILATEDGGFLLIGESNSNSGFDKSENGFGGYDFWVVKIDAMGTVVWNKTYG
                     GNLDDLPLSIFQTTDDNYIIAGSSTSGVSGNKTTPNKGNSDFWVIKIDNVGNVLWQRS
                     YGTSKLDHFSDAEQTIDGGIILVGYTNAGITGDKTVATFGMNDYWVIKTNAIGDIQWQ
                     LGAGGNKDDWADAVSISNSGNIYIGGSSMSGVSGNKTVASLGGTFDSWILKLDASGNI
                     VWQKDIQGNDSDYFGDMVVNDENELVAAVYSFSSNSNDKFEPWYGLYDYWIFKLSDTC
                     NAEFCNGMDDDCDGLIDDATMDTIYIAANEGTSCCAGGNVKLQAYHSSGTIQWLRNGN
                     PIPGATGDIYFATKKGNYSCKLNIACELVTSNIIEVKVYKCPVGNIVADGPTTFCDGD
                     SVVLSCTNLPEEDYTYQWYNGMAFIPGALGSTLTVFEPGTYKCLVIRNVGGCGDFSNI
                     IDVHVTCKTSSELSELQIAPNPSNGKFTCSVDPEFGQIQQCKIVDVAGQVVYNVLLNG
                     GADFTIDTSLPTGIYQVLIFNDYGEMQSKPLVIE"
     gene            complement(5044..6030)
                     /locus_tag="PLL28_08345"
     CDS             complement(5044..6030)
                     /locus_tag="PLL28_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69369.1"
                     /translation="MNEEKKSGTRGRVLYLLLILLLLGMNGWLFFNAAQNKDDREKSA
                     RLLEEAKIVNAQLEQQYQTAKKQLDDQKGDFTQKDSLIAVLEAELTSRRNEINALLKT
                     CDFFKGNVKTNEKKIDEVKEEVKTMEVDCSSYKAQLDELNARYLALQEDYEELQFLYE
                     QEVERSEQLQFDKDSILDLAGFILSKDINITGVRKKGNGNDSEGQNAKHTEMLKVCFD
                     VLPNRLSAGKNQTFLLRIISPTGKVLYDQTSGSGEFVNKEKPEDNTYTTSLTVKYDGG
                     EVESHCVYWEQSDEFKPGTYTVKIYHNGYLSSRSSFTLKKPVMEDLMDKVKG"
     gene            complement(6569..7717)
                     /locus_tag="PLL28_08350"
     CDS             complement(6569..7717)
                     /locus_tag="PLL28_08350"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNF69370.1"
                     /translation="MQQRIIVKTFQGFESILASELFALGATDVEVLNRAVQFKGDKAL
                     LYAANIHLRTGLKVMIPLLEGRARNEQQLYDLVKQFDWGDVMRVDDTFSISQAIYSRF
                     FPHGNYAALKIKDAIADHFRDAFGMRPSVDRDNAVIQLHVHIAEDQIQISLDSSGDSL
                     HLRGYRTGTHAAPMSEVQAAGLILLSGWDQKTTFYDPMCGSGTMPIEAALIAANVAPG
                     LIRKHFAFQRWHDYDTALYRQLILDAAAKVKRNKLHVLGSDINGRNVDLAQAHAIAAL
                     PDMKIPFFKKDFQLADPPPEKGVVIMNPPYGERLQHADMIAFYQMIGNVLKQKYAGWT
                     AWVFTGNLEAAKFIGLKPSEKYKLKNGPLDALFLRFDIREGKFWKGQG"
     gene            complement(7788..8324)
                     /locus_tag="PLL28_08355"
     CDS             complement(7788..8324)
                     /locus_tag="PLL28_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69371.1"
                     /translation="MKRILNICLMVAFIGLAAVKSNGQVVMKDFLTEDHVGKVDKAAN
                     MNGGALYYKFEYVSTEGARINYKLHLYKDKGMSTPWMSFDVLMRNLNWTYYVDITMSK
                     DGADKVAALIFKKDLRWGRVKFSPHEGCGRTNPPVWERYTIGEQKGTPEELYAGLLNN
                     YLMQLDKNVDFNCYAGSK"
     gene            complement(8348..9742)
                     /locus_tag="PLL28_08360"
     CDS             complement(8348..9742)
                     /locus_tag="PLL28_08360"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HNF69372.1"
                     /translation="MKLKHLPVVVLTGAVLFATGCGKKSGGGENPLYNDASKPSSEES
                     AAFLLDWWNAMFQFVASERLSPPDAARMYAYISVGLYEAQIAGTPDYLTLEGQLTDLK
                     GLPRPEKGQVYDWQTCVTETMYLVQDGMLARYFPAGVSTLNKLYDKQMESRKAAGVTD
                     EVIERSKAYGEELADAILEWSENDGYVQTRYKQYKAPSREGHPELWEPTDFNQTALEP
                     FWGEHRPFFIKSASQCDIDKAPAYHGVGDTTGELYKQALKVWQVDKTLTEEQRTIAQF
                     WADDPSETSTPPGHWMEILGNFVKSEDMKLDRAAELYALTATAMNDACIAVWYTKYRV
                     NLVRPKTVIHENIEPTWEPYVETPPFAGYTSGHSGFSGAAATIMSGLIGDNLAFTDSS
                     HVNIGLLPRNFASFQAAADEAAYSRMYGGIHFDCDILDGVEQGRCVGNYYLENIKTHP
                     ARKTAAKGEAKKEE"
     gene            9902..13504
                     /locus_tag="PLL28_08365"
     CDS             9902..13504
                     /locus_tag="PLL28_08365"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNF69373.1"
                     /translation="MKASQSVILGLSVLTILSSCGSNKGSKEYLNDWKFTENIDKAKI
                     VQTDGESGKMFDLLSSKTTGINFNNELIETFDLNYYRYGYSYNGAGVGVGDFNNDGLQ
                     DLYFAGNVVPDRMFLNKGGMQFEDVTAKSGINKKPGWSTGVTVVDINEDGWKDIYVCR
                     ARFEDPEKRRNLLYVNNGDGTFTEKAKEYGIDDDSYSTHANFFDADNDGDLDLYVVTH
                     PTDFKDKNKQKNFQKIEEGTNMSNRFYRNEGNGKFTECHKEVGINNHGFGLSVTTGDI
                     NDDGWMDVFVGNDYIMHDYTYLNQGNGTFKESSRDVLMKTAYYGMGTDLADYNNDMLL
                     DMFTVDMDIEGNYGTKTFMQSNKQTFLRTLVNGGYLQQYGRNALQLNNGVGKFSEVAN
                     FAGVSTTGWSWSPLFADYDNDGYKDLYVSNGFLKDSHMDIMEVYVKLTRANRLSDSTE
                     YYELRKQIPESSVLEWPNAMFHNNGDLTFTDVREDWGLYFPSTSYGAAYADFDNDGDV
                     DIVVSNANMEAFIARNNSSKLNTNKYLRVDLKGEKGNPEGLGAKVYVTTSTGTQMVQM
                     ETVKGYLSCSEPVAHFGIGSAEKVDQLVVRWLDGKENVLTNVAANQTVVVDHSTAMKP
                     IKSAVPKPDALFVNATEDLKVDFVHKENEFDDFDREFLIPKELSNLGPGVAVGDINGD
                     GMDDFFVGGAKNQSGAIYTQTANGTFAKTSFAEGTEDIQSEDMGAIFFDADGDNDNDL
                     YVVSGGSDFPKDDVHLQDRLYINDGNGKFSWAKDALPKMISSGSVVAAQDFDKDGDLD
                     LFVGTRIIPGNYPYPTKSYILQNNKGKFTDITSSVAPDLEKIGLVSAAIWTDVNNDGD
                     YDLMLTGEWMPITIFENNGGKFKNITNAAGLSESTGWWNSLTAGDFDNDGDMDYVAGN
                     FGTNLKYRPKEKPIELFYDDYDNNGTHDIIMGYWQHDKLYPEKTRERMIEQIKDVETI
                     FPDWDSYGRAEVWDFYGKKRMENSQLHYKANSFYTSYIENQGNNKFAVKRLPNDIQIS
                     STFGIVPMDVNQDGNLDIVAHGNFYETEIETNTQDAGIGNVLLGNGDGTFTPLHARYS
                     GFYSAMNAKSLAMIKVGAAKTPVLLTTNSNNKMEAFKLAKNTAVGVNANDAYAVITFS
                     DGKQRKQEFYNGAGYLSQSARAVVVNDAVSQVVVYDNKGTARTIFGQQVSMNKK"
     gene            13532..>13819
                     /locus_tag="PLL28_08370"
     CDS             13532..>13819
                     /locus_tag="PLL28_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69374.1"
                     /translation="MMKIHNVSVLIAAFAMFTVASCKLFSGGDVDTRGTAIIDPKREV
                     KLDGNNGKLFDILTDKSTGITFVNDLTENYSVNWWRYSYIYNGGAVCIGDVN"
BASE COUNT         4183 a         2998 c         2698 g         3940 t
ORIGIN      
        1 aaaaggaagc cttctggcac cttattatgc acatattctg ttggatgata ttctgcataa
       61 cacaggaatt ctcgcaccag ccgatgtgca gcgcactttc cgttaacaaa ccttatttgt
      121 cggggcgggg gtgaagaatt atttttacct gatgaagaag ccggtatttt ctttgttttc
      181 attcgttgcc atggcaatat gcagcacctt aagcataaac gctcagaata ctacatttaa
      241 ttgctaccta cctgaccaag gtggaagcat tcgcgaacac aatgtaaatt ttatttccct
      301 tgacctagat gtgaagtttg caccggaaga cggattggta agcggcattg ctcgatacgc
      361 tttcactgcg ctcgaaccaa ctgttgattc agtatttctt gatggaccag gcatcagagt
      421 gcaggaaatt tctcttgaca acaatgctac taaattcaaa atggatagcg ccggtatcac
      481 tgtttacttc aacccaccac taatacgaga agcagtacat aatatggcca tcatctacga
      541 tgcgaaccct aaaaaaggta tctatttcaa tggttggaat gttgcttcaa ctgataatcc
      601 gaatgatcct actatcatta gaaaacaaat ttggacacaa ggtcaaggcg tagataacag
      661 attttggatt ccttcttacg acggagtgaa tgacaagctc ctcaccatga tgcatataac
      721 gtttgactcc acatatacag ttgtatcaaa tggcaatcta aaaaatgtaa gcgcgaatgc
      781 tgatggcact aaaacatggc gatatgctat gccacatcct catgcactat atcttgtcat
      841 gttggctata ggcaaatacg atgtgatgga attcacttct aaaaatggta tcactacacg
      901 ccaatattat tatcccggaa cgaaagaata tgctgaaaac acttatcggt atactgccga
      961 attaatggat tggatgcaac aggccaccgg attaaaatat ccatggtcta cttatgcaaa
     1021 tgttcccgta caggaatttt tatatggcgc catggaaaat accaccgcta ctattttcag
     1081 cgatttcttc tatcaacgcc ctgctagcaa tcctgataaa caatatatcg aaatcaatgc
     1141 acacgaatta acacatcaat ggtttggcga ttacgtaact gcatggagcg gatccagtca
     1201 ctggttgcag gagagtttcg caacatattt ctcgaagcat tttgcccaac atattaacgg
     1261 cgatgattat tatgactgga aaagacgcga agagatgcta actgctttag atgcagacag
     1321 cagagataat attccaattg cgcattctga agcaggctca cagcgcgtat atcaaaaagg
     1381 cagttttgta attgatatgc tgagatatgt tgttggtgac caggcattta atgtggccat
     1441 tcaggaattc ctagaacgct atccttatgc gaatgtaagt acacaagatt ttcaaatgca
     1501 atttatgcgc tcattgggaa tgaatatgga ttggtttttt gatgaatggt tatatagagc
     1561 cggattcccg gtttataccg tgaaatatga tacaaccgaa aatgctaccg tagtgcatgt
     1621 ttcacaaacc cagcaaacaa atgaaacggt gcacttgttc aatatgccga ttcatattca
     1681 gattcactat atggacggaa gttttgatga tcagttggtg gtaatcaaaa atgctgcaca
     1741 ggatatcacc attcagaatc cggaacacaa gcaagtgctt tttgtacttt tcgatccgaa
     1801 tcatatggta ttgtcgaaag tagtttttga taaatccttc aacgagctca cctatcaggc
     1861 ttttaatgcg ccaaatatga tcgacagata tgaggcgata tccgcaatga ccgaaacgcc
     1921 gattgaagta aagagagtaa cattgctggc actattgaaa aaagagaaat ttaataatat
     1981 caggatagaa atcattcgac aattaagcag cgacaatgat aaggcaactg tagcagcctt
     2041 aaaaaatgca atttatgata cagaccctct tgtaagaaga tcggtggtag taaattggaa
     2101 caaggataat gacaagctga gaaaggatat cgaaacactt ttaaaggacg ataattatgt
     2161 gaatattgaa ttagcacttc gtaagctatg tgattattac ccaaaacaaa cttcagaata
     2221 tttacaaaaa acagccggac aatttggagc aacaaataac attcgcgtgg cctggctgga
     2281 attgcaaaat aaggataaca ataatggcac gatgagcgaa ttgattaaat tcacgagccc
     2341 tcaatatgaa ttcagaaccc gtgtggcagc attcaatgcc atcagcaatc gcaattattg
     2401 cgatcctgag ttagttgcga atttattcaa tgcacttgtg agcaccaatc accgattatc
     2461 atctccggca agaaacctgc tgatggcata caagagcaat cctgaatatg ccactatgat
     2521 aaaagcatat tataattcca gactctggac tgaatgggaa gacaccagga tcggaaaggt
     2581 gaaggaataa actctactca atcacgagtg gtttagactg catttcgcca tagtcattga
     2641 atatcaatac ctggtatatt ccggttggaa gactcgtgtc aattgtaaaa tcggctcccc
     2701 catttaatag gacgttgtat acaacttgac ctgcaacatc aactatcttg cactgttgaa
     2761 tttgtccaaa ttcaggatct accgagcagg taaattttcc atttgacgga ttgggagcaa
     2821 tttgcaattc tgaaagttcg gaagaagttt tacaagtaac atgcacatct ataatattag
     2881 aaaaatcgcc acaccctcct acattacgaa ttacgagaca tttatacgtt cccggctcga
     2941 aaactgtcaa tgtgctgcct aaagcacccg gaataaatgc cattccatta taccattgat
     3001 aagtataatc ttcttcaggc aagttcgtac atgaaagaac tacactatca ccatcacaaa
     3061 aagttgtcgg accatcggca acaatgtttc ctacagggca tttataaact ttgacctcaa
     3121 taatgttaga tgttaccaat tcgcatgcaa tatttaattt acaggaataa tttccctttt
     3181 ttgtggcaaa atagatatcg ccggttgctc ccgggatagg attaccgttt cttagccact
     3241 gaatagtacc ggaactatga tatgcttgca atttaacatt accgcctgcg caacaggaag
     3301 tgccttcatt agctgcaata taaatagtgt ccattgttgc atcatctatc aacccatcac
     3361 aatcatcgtc cataccattg caaaattcgg cgttacaggt atctgataat ttgaatatcc
     3421 agtagtcgta aagtccatac caaggctcaa atttgtcatt agaatttgac gagaatgaat
     3481 atacggcggc aaccagctca ttttcatcat ttaccaccat atctccaaaa tagtcgctgt
     3541 catttccttg gatgtccttc tgccaaacta tatttcctga tgcatctagt tttaatatcc
     3601 aactatcaaa tgttcctcca agcgatgcaa cagttttatt tccggataca ccggacattg
     3661 agctgccacc aatataaatg ttacctgaat tactaataga aacggcatct gcccagtcat
     3721 ccttgttacc acctgcgccg agctgccatt gaatatcgcc tattgcattc gtcttaatta
     3781 cccaataatc gttcattcca aatgtcgcca ccgttttatc tccggttata cctgcgtttg
     3841 tatatccgac caatatgatg cctccatcaa tcgtttgttc cgcatctgaa aaatggtcca
     3901 attttgaagt gccataagaa cgttgccaca acacattgcc aacattatca atcttaatca
     3961 cccaaaaatc actgtttcct ttattgggcg tggttttatt tccggataca ccggatgtgg
     4021 aggatccggc aatgatataa ttgtcatcgg tcgtttgaaa aatacttaat ggtagatcat
     4081 ccaaattgcc gccataggtt ttattccata ctactgttcc catagcatca attttgacaa
     4141 cccaaaaatc atatccaccg aaaccattct cagacttgtc aaatcctgaa ttagaatttg
     4201 attcaccaat caaaagaaat ccaccatctt cagtagctaa tatattacgc atctgatcag
     4261 atcctgttcc tcctattgta ttttgccagg aaatattacc ggatgcgtca agttttaaca
     4321 cccaaaaatc tacatcacct tctcccccta tactcgtctc tgttttatca ccggttgctg
     4381 tagattgcga agtacctcca acaatacaac cgccatcagg tgtgctgcaa acaactgaat
     4441 ttgtttcatc gcgtttacct ccaattgtgt tctcccataa cacataaccg gaattgctca
     4501 ttttcacaac ccaataatcg taataatcaa attcaccctt attattttca cttttatcag
     4561 gtgaagattc cgaattcgaa ataccggata tgaaatacgc cccatcggca gcattgacaa
     4621 cagaatatgg caaatctgat ttactcccct gaagcgtgtt ttgccataat atacccgggg
     4681 cctgactata taaaaattta actgtcaggc aaatggctaa aacaaaaaag gaaactgctt
     4741 tcataaacat tacaatttat tacttttaat aaaattgatt cgtcaaagct acatggacga
     4801 ttgcaatagc accaaagaag aattcacgat atttgggtat aatttcaaat tatttgctaa
     4861 atccgggata ttaacaattc aaaaattaac cagaaaaaag caatatattt tctaatgcag
     4921 ctgccgacaa ttaatttctg ataaaattaa atattcacgc ataacaaaag gctcatcaaa
     4981 atcatcctat aaaaaaaccc cggtgttacc cgaggtttct cctttcatac ttatagcaaa
     5041 aagctaccct ttaactttat ccatcaagtc ttccattacc ggttttttca aggtaaatga
     5101 actgcgactt gaaaggtatc cattgtgata aatttttacg gtataagttc ccggtttaaa
     5161 ttcatctgat tgttcccaat atacacagtg actctcaact tcacctccgt catattttac
     5221 tgtaaggctg gtggtataag tattgtcttc aggtttttcc ttattcacaa actctcctga
     5281 gccacttgtc tgatcgtata acactttacc tgtcgggctt atgatacgca gtaaaaatgt
     5341 ttgattctta cccgccgata atctattcgg caacacatca aaacatactt tcaacatttc
     5401 agtatgcttc gcattttgtc cttctgaatc attgccattt ccttttttgc gaacaccggt
     5461 aatattgatg tctttcgaca aaatgaaacc ggcaagatca agaatcgaat ctttgtcaaa
     5521 ttgcaactgt tcactgcgct caacttcctg ctcatacaag aattgcagtt cttcataatc
     5581 ttcctgcaat gccagataac gcgcgtttag ttcgtcgagc tgagccttgt aagaactaca
     5641 atctacttcc atcgtcttca cttcctcttt cacctcatca attttcttct cattggtttt
     5701 tacattgcct ttgaagaaat cacaagtctt caacagggca ttgatctcat tccttcgaga
     5761 agtcaattca gcctccaata ccgcaataag actgtctttc tgggtgaaat cacctttctg
     5821 atcgtctaac tgcttctttg cagtctgata ctgttgctcc agttgtgcgt ttacaatctt
     5881 ggcttcttcc aacaaacggg cactcttctc gcggtcgtct ttgttttgag ccgcattgaa
     5941 gaacaaccat ccgttcatcc ccagaagaag taggatgagt aacaagtaca gcacccttcc
     6001 acgggtaccg cttttcttct cttcattcat acaagacagg caggattttt cgccattgcg
     6061 gttgaaccgg accgcagata gcccccttgc cttattcaaa attgaatgaa accactatct
     6121 caacgaaata atctaatgca cacagtactt tggtacagag gcatttatgt ggtacaacta
     6181 caatatccac acacatgtac ctaccccttt cgaaatttaa accccttgtc agtacagggt
     6241 ttcaggtaca acagacgcct tgcggtacat gggattcagg agttgtgtac acaattttcg
     6301 gcatatgtgg ataacctgtt gaaaaaggag gagagaggag aaaggagaaa ggagagtggg
     6361 tgagtgggtg agttttatta aaggcaaaaa aaatcccgac cgcaggtcgg ggaaataact
     6421 caccacccgc cttctcggcg aggtaaactc acccactgac ccactcaccc actcacgatt
     6481 cacgattcac gattcacgag tcacgattcg gtagtgggta ttgagtagtg agacaaatgc
     6541 acccactcac cactcaccac tcacccactc acccctgccc cttccagaac ttaccttctc
     6601 taatatcgaa tctcaaaaac agggcatcca atggtccgtt tttgagtttg tatttttcgg
     6661 atggtttgag gccgataaac tttgctgctt ccagattacc ggtgaaaacc caggctgtcc
     6721 aaccggcata tttttgtttg agcacgttgc caatcatctg ataaaatgct atcatatcgg
     6781 cgtgctgtaa tcgctcacca tagggcggat tcataatcac aacacccttt tctggaggcg
     6841 gatctgcaag ctgaaagtct tttttgaaga atggtatctt catgtcagga agcgcggcaa
     6901 tagcatgcgc ctgtgcaaga tccacgttcc tgccattgat atctgatcct aacacatgta
     6961 atttatttcg cttcacctta gctgcagcat ccaaaataag ctgtcggtag agagcggtgt
     7021 catagtcgtg ccaacgctga aatgcaaagt gttttctgat gagtccggga gcaacattcg
     7081 cagcaataag cgctgcttca ataggcatag tgccgctgcc acacataggg tcataaaatg
     7141 tagttttctg atcccaacca ctcagcaaga tcaagccggc tgcctgcact tcactcatcg
     7201 gtgcagcatg agttccggtg cggtatcctc tcaagtggag actgtcgccc gagctatcaa
     7261 gtgaaatttg aatctggtct tcagcaatat gcacgtgtaa ttgaattact gcattatccc
     7321 tgtccacact tggtcgcatt ccgaaagcat cacggaaatg gtctgcaatg gcatctttga
     7381 ttttcaatgc agcataattt ccatgcggaa aaaacctcga ataaatggct tgagaaatac
     7441 taaaagtatc atcaacacgc attacatcac cccaatcgaa ttgtttcacc aggtcgtaca
     7501 attgttgttc attgcgggct ctgccttcta aaagcggtat catcactttt aatcccgtac
     7561 gcaggtgaat atttgcggca tataacaggg ctttatcacc cttaaattgc acagccctgt
     7621 ttaacacctc aacatccgta gctccaagcg caaacaattc tgacgccagt atgctttcaa
     7681 atccctgaaa tgtttttact ataatgcgtt gttgcatatg tgggtgtaaa aataagaaag
     7741 gcgttgttgt gcacaacgcc tttcatgaat gtattagata cggcagatta tttacttcct
     7801 gcgtagcaat taaaatcaac gtttttgtcg agctgcatca ggtaattatt caaaagaccg
     7861 gcatatagct cttcaggtgt tcctttttgt tcgccgatgg tgtaacgctc ccatacaggt
     7921 gggtttgttc ggccacaacc ttcatgtggc gagaatttca cacgacccca acgaaggtct
     7981 tttttgaaaa tcaatgcggc taccttgtct gcaccgtcct tagacatcgt aatatcgaca
     8041 taataagtcc agttaagatt gcgcatcaac acatcaaagc tcatccatgg cgttgacatg
     8101 cctttgtctt tatacaaatg caatttgtaa ttgatacgtg ctccttcagt acttacatac
     8161 tcgaatttgt aatatagagc accaccgttc atattcgctg ccttatcaac tttacctaca
     8221 tggtcctctg taagaaagtc tttcatcacc acctgtccgt tagacttaac agcggcgagg
     8281 cctataaagg caaccatcag gcagatattt aatatccttt tcattgtttc tgtggtttaa
     8341 tggtttatta ttcttctttt ttggcttcac cttttgcagc agttttgcgt gcagggtgtg
     8401 ttttaatatt ttcgagatag tagttaccta cacaacggcc ttgctcaaca ccatcaagaa
     8461 tgtcgcagtc gaagtgaata ccaccataca tacgactgta agcagcttca tcagcagcag
     8521 cctggaatga tgcaaaattg cgtggtaata aaccgatgtt tacatgtgaa ctatctgtaa
     8581 atgcaaggtt gtctccaatc aatccggaca tgatggtagc cgcagcacca ctgaatcctg
     8641 agtgacctga agtatatcct gcaaatggag gtgtctcaac atatggttcc caagttggtt
     8701 caatattttc atgaataacc gtttttggtc gaaccagatt tacacggtat ttagtatacc
     8761 ataccgcaat acaggcatcg ttcatcgctg tagctgttaa cgcatacaac tcagctgcac
     8821 ggtcgagttt catgtcctca gacttaacga aattaccaag gatttccatc cagtgtccgg
     8881 gaggcgtaga cgtttcactt ggatcatcag cccagaattg agcgatggtg cgttgttctt
     8941 cagtcaatgt tttatccacc tgccatactt tcagtgcttg tttgtaaagc tctcctgtag
     9001 tgtcaccaac tccgtggtaa gcaggtgcct tatcgatatc acactgtgaa gcagatttta
     9061 taaagaaagg acgatgttca ccccagaatg gttccaacgc tgtttggttg aaatctgttg
     9121 gttcccacaa ttccggatgt ccttcgcgtg aaggcgcttt atattgcttg tagcgagttt
     9181 gcacgtatcc gtcgttttcg ctccattcaa gaatcgcgtc tgcaagttct tcaccataag
     9241 ctttagaacg ctcaattact tcatcggtaa caccggcagc tttgcggctt tccatttgct
     9301 tatcgtataa tttattcaac gtacttaccc ctgcagggaa gtaacgtgcc aacataccat
     9361 cctgaaccaa atacatggtt tcggttacgc atgtttgcca gtcatatact tgaccttttt
     9421 caggtcttgg aagacctttg agatctgtaa gctgaccttc aagagttaaa taatcgggcg
     9481 taccggctat ctgtgcttca tataggccta cagaaatgta tgcatacata cgcgcagcat
     9541 ctggcggaga caaacgttcg ctggctacaa attggaacat cgcattccac caatcaagaa
     9601 ggaaagcagc gctttcttca ctggaaggtt tggaagcatc gttgtaaagt gggttctctc
     9661 ctccaccgga tttttttccg catcctgtgg caaaaagcac ggcacctgta agtacaacga
     9721 caggtaagtg ttttaatttc atgacttcta agattttagt ttagcagttg agtgattgaa
     9781 ataatcgctg caaaattaag caaatttagc catcggcacc ccattaacaa aacatttaca
     9841 gaggtcgtac ttacccccct tttaagcagg ttacctttgc agttcaaacc gtaaacaagg
     9901 catgaaagct tcacaatccg taatccttgg cctaagtgtg cttaccattt taagcagctg
     9961 tggttctaac aagggcagta aagagtatct caacgactgg aaattcactg agaacatcga
    10021 caaagccaaa atcgtacaaa ccgatggcga gtcaggaaag atgtttgatc tgctttccag
    10081 caaaacgaca gggatcaact tcaataatga acttattgaa acctttgacc tcaactatta
    10141 ccgctatggc tattcctata atggcgccgg tgtaggtgtg ggcgatttca ataatgatgg
    10201 tcttcaagac ctgtattttg caggaaacgt agttccggat cgcatgttcc tcaacaaagg
    10261 tggcatgcaa ttcgaagatg ttactgcaaa aagtggtatc aacaaaaaac cgggctggag
    10321 taccggtgtt accgttgttg acatcaatga agatggatgg aaagacatct atgtttgtcg
    10381 cgcacgattc gaagatcctg aaaaacgccg caatctgtta tatgtaaata acggcgatgg
    10441 cacttttact gaaaaagcaa aagaatacgg tatcgacgat gatagctatt caacgcatgc
    10501 caacttcttc gatgccgata acgacggaga tcttgatttg tatgtagtaa cccaccctac
    10561 cgatttcaaa gacaaaaaca aacaaaagaa cttccagaaa attgaagaag gtaccaacat
    10621 gagtaaccgc ttctatcgca atgaaggaaa tggcaaattc acagaatgcc ataaagaagt
    10681 aggtatcaat aaccacggat tcggattatc tgtaactaca ggtgatatca acgacgatgg
    10741 ttggatggat gtgttcgtag gaaacgacta catcatgcat gactatactt acctgaacca
    10801 aggtaacgga acctttaaag aatctagccg cgatgtgttg atgaaaacag catattatgg
    10861 tatgggtacc gatctggccg actataataa tgacatgctc ctcgatatgt tcactgttga
    10921 tatggacatt gaaggaaatt acggcacgaa aacatttatg caatctaata agcaaacatt
    10981 cctccgcaca ttggtgaacg gtggttattt gcaacaatat ggtcgcaatg cattgcaatt
    11041 aaataacggt gtcggtaaat tctccgaggt tgcaaacttt gcaggtgtat ctactaccgg
    11101 ctggagctgg agcccattgt tcgccgatta tgataatgat ggttataaag acctttatgt
    11161 atcaaacggc ttcctgaaag atagccatat ggatatcatg gaagtttacg tgaaattgac
    11221 tcgcgcaaac cgcttatctg attctacaga atattatgag ttgcgtaaac aaatacctga
    11281 aagctcagta ctcgaatggc ctaacgctat gttccataac aatggtgatt taacgttcac
    11341 tgatgttcgt gaagactggg gattgtattt cccatctact tcttatggcg ctgcctatgc
    11401 cgattttgat aatgatggcg acgtagatat cgtagttagt aatgcaaata tggaagcatt
    11461 tattgcccgc aataacagca gtaaattaaa taccaacaaa tatttacgtg tcgacctcaa
    11521 aggcgaaaaa ggaaatcctg aaggccttgg agcaaaagtg tatgtaacaa cttctacagg
    11581 cacacaaatg gtgcaaatgg aaactgtgaa aggttacctt tcatgttcag aaccggttgc
    11641 ccatttcgga attggctcag ctgaaaaagt tgatcaatta gttgtacgtt ggctagatgg
    11701 aaaagaaaat gtgctcacaa atgttgctgc aaatcaaaca gttgttgttg atcacagcac
    11761 tgcaatgaaa cctataaaat ctgctgttcc gaaaccggat gcattatttg taaatgcaac
    11821 tgaagatctg aaagttgatt tcgtacataa agaaaatgaa ttcgatgatt tcgatcgcga
    11881 attcctcatt cctaaagaac tcagcaatct cggtcccggt gttgctgttg gagacatcaa
    11941 tggtgacggc atggacgatt tctttgttgg aggtgctaaa aatcaaagcg gtgccatcta
    12001 cacacaaact gcaaatggca catttgcgaa aacttccttt gctgaaggaa ctgaagatat
    12061 ccaaagtgaa gacatgggtg ccattttctt cgatgctgat ggtgataatg ataacgacct
    12121 gtatgttgta agtggcggtt cagatttccc taaagatgat gtgcatttgc aagatcgttt
    12181 gtatatcaat gatggaaatg gaaaattctc atgggctaaa gatgcattgc ctaaaatgat
    12241 ctccagcggt tcagttgtag ctgctcagga tttcgataaa gatggcgacc tcgacttgtt
    12301 tgttggaaca cgcatcattc cgggtaatta tccttatcct accaagagct atattctcca
    12361 aaataacaaa ggtaaattca cagacattac gtcgtctgtt gcacctgatc tcgaaaaaat
    12421 cggacttgta tcagcagcta tttggactga tgtaaataat gacggtgatt atgacctcat
    12481 gcttaccggt gaatggatgc cgattactat tttcgaaaac aatggcggta aatttaaaaa
    12541 tatcactaac gcagcaggcc tttcagaatc taccggctgg tggaatagtt taactgccgg
    12601 tgatttcgat aacgatggcg atatggacta tgttgccgga aattttggta ctaacctgaa
    12661 atatcgtccg aaagaaaaac caatcgaatt gttctatgac gattacgata ataacggcac
    12721 acacgatatc atcatgggtt actggcagca cgacaaacta tatccggaaa aaacacgtga
    12781 acgtatgatt gaacagatca aggatgttga aactattttc cctgactggg atagctacgg
    12841 acgtgctgaa gtttgggatt tctatggtaa aaaacgcatg gagaattctc aattgcacta
    12901 caaagcaaat tcattctata ccagctatat agaaaaccaa ggcaacaaca aatttgcagt
    12961 gaaacgctta ccaaatgata tccagatatc atcgacattc ggtatcgtgc caatggatgt
    13021 aaatcaggat ggcaatctgg atatcgttgc gcatggtaac ttctatgaaa cagaaattga
    13081 aactaacact caagacgccg gtatcggtaa tgtgttgtta ggaaatggcg atggcacatt
    13141 tacaccatta cacgcacgtt acagcggatt ctattctgcc atgaatgcaa aatcgcttgc
    13201 catgataaaa gttggtgcag ctaaaactcc ggttttactt acaaccaatt caaacaacaa
    13261 aatggaagcc ttcaaactgg caaaaaatac cgcagtaggt gtgaatgcca acgatgcata
    13321 tgctgttatc actttcagtg atggcaaaca acgcaaacag gaattctaca atggagcagg
    13381 ttacctcagc caaagtgcgc gcgccgttgt tgtaaatgat gctgtatcgc aagttgtggt
    13441 atatgataat aaaggcactg ctcgcacaat ttttggccag caagtttcaa tgaataaaaa
    13501 ataaacccct aatcaaccgc tcaatacaac aatgatgaag attcacaatg tatcggttct
    13561 gattgcagca tttgcgatgt tcacagtggc atcgtgcaag ctgttttcag gtggcgatgt
    13621 cgatacgcgg ggtaccgcga tcatcgaccc aaaacgcgag gtgaaactcg acggtaataa
    13681 cggaaaattg tttgatatcc ttacagacaa atcaaccggc attactttcg taaacgacct
    13741 cactgaaaat tacagtgtaa actggtggcg atattcatat atttataatg gcggtgcagt
    13801 ttgtatcggc gatgtaaat
//
LOCUS       DAOQHY010000085        13678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2168_length_13678_cov_5.086325, whole genome shotgun sequence.
ACCESSION   DAOQHY010000085 DAOQHY010000000
VERSION     DAOQHY010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13678
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2168_length_13678_cov_5.086325"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1695
                     /locus_tag="PLL28_08375"
     CDS             <1..1695
                     /locus_tag="PLL28_08375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNF69375.1"
                     /translation="SEYGNEGTWEKITIIDFPFDNYTGTVQTDVDGDLITDTIGTTDG
                     SHFMTITDDGTVHVFSPYYRIYSDLGAFFWTVNWNTMGLWHWSSGMPAAEVIDLEMDW
                     INADCSNDPYVGIGASTFNYRNAAYVTDPGAAYDPISGRMFLLYTMKIEYTDVFGDPT
                     NPSAQSFHDIFGTYSDDGGATWAQPVNLTNTAETGEENFFMYVYDRVVDGKVYAVWQQ
                     DGEPGHFNEGDPITVNNIKFRAWDIESFNPTLPTAAYLFVTDIGEVTFTNISEDATGC
                     NFWDFGDGTTSNESDPVHIYTVAGEYTVCLTAENPYGTDTYCGTIYISLPPDAAFSYT
                     GDPIVTFTDLTLNDPTSWSWNFGDATTSTLQNPVHTFTSEGTFTVCLTATNALGFEVY
                     CLPVTIDSTELLAPAADYSFTVAGGTTVNFTDMSTNAPTSWAWDFGDGGSSTEQNPSH
                     AFPSSSSYEVCLIAANAFGSDTVCKTVLGSEVIDLSDMITLQPNPAHDMVTIHTGTFH
                     PDMIHVYDITGREVMVEVQLSANDTYVMQVSSLPQGAYIVHMQHENESGYARLIVQ"
     gene            complement(1732..3810)
                     /locus_tag="PLL28_08380"
     CDS             complement(1732..3810)
                     /locus_tag="PLL28_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNF69376.1"
                     /translation="MNQKETLEMIDRKVLLEAYRKMYTSKVMSDIFNENRQVCVFVHA
                     NSRGHEAIQLAMGMQLKKSDWASLYYRDDAFLMGMGWQPAQIMLQLMAKRDDPFSGGR
                     NYYCHPSYKGPDYPQMIHQSSATGMQAIPTTGVAQGIQYLEQQNLLATSNDHPLVVCS
                     IGDGAMTEGEVSEALQFSVLKGLPIIYLVQDNDWGISVSREEARTMDAYEYAGGFKGL
                     RRVRVDGADFAESWQCVKDAIGYVRVHRKPVLIHAKVPLLGHHTSGVRMEMYRSEEDL
                     KEHASRDPLPRLRQVLVDAGISLQEITTIETEADQQTRADFQTVLASADPDPATVSDF
                     EFAPSPYTEEQGTREPQGADVVPMVDAALFAMDEILTKHPEALFYGQDVGRRLGGVFR
                     EAATLAAKHGDHRVFNTAIQEAYLVGSTAGMSAVGAKPVVEIQFADYIWTGVNQLVSE
                     LSKSYYLTNGKWNVQSIIRVPIGAYGSGGPYHSGSIESSITAIKGIKVVYPSNAADMK
                     GLLKGAFYDPNPVVMFEHKGLYWSKVPGTAAAKNIQPADDYIIPLGKARIFQHADDKH
                     IHNGESMVVITYGMGVHWALNASKQFPGQVEILDLRTLYPYDWEAITASVKRHNKCFV
                     LTEEPLINSYAQAMSGRIGSELFRYLDAPVRTLGAKDLPAVPLNSGLERAMLPNVEQV
                     KTVMQELLSF"
     gene            complement(3830..4438)
                     /locus_tag="PLL28_08385"
     CDS             complement(3830..4438)
                     /locus_tag="PLL28_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNF69377.1"
                     /translation="MKIFCVGRNYGEHAKELNNAIPEAPVIFMKPPTAILKGNDFYMP
                     DFSKDMHFECELVFRICKNGKHIAPQFASKYVDGITVGIDFTARDVQAEQKKKGLPWE
                     IAKAFDQSAVVGTMQPLEDMSLLSRASFELKKNGVAVQTGNAGEMLFPVPEVISYLSN
                     YFTLQQGDLLFTGTPAGVGPIAIGDVLEGSLNGNVLFTTHIK"
     gene            4509..5432
                     /locus_tag="PLL28_08390"
     CDS             4509..5432
                     /locus_tag="PLL28_08390"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF69378.1"
                     /translation="MRFSIKHLILSSFCLLMMLPSLQAQRQLGSKPMGNGQPGSAGKS
                     FDHIEGTYAVVNGINMYYEQYGEGDPILLIHGNGGSTESFDGQIAFLSRKYKVIVADS
                     RGQGRSTNNADSLTYELMAEDFYLFLEQLELDSVNIVGWSDGGIIGLILARDYPEKVK
                     KLVAVGANLRPDTTAVSEALLDWTRMKMEQAQDSVAAGKKSFATEEMLMRLMLHHPNI
                     DVADLHDIKAPVMVVAGDHDLIKVTHTVEIYENLPHAQLAIYPGATHNLMLESPQLFN
                     NAVLRFFSRPYVAKDPMSVMLDTPSKNNDEE"
     gene            complement(5449..6072)
                     /gene="pth"
                     /locus_tag="PLL28_08395"
     CDS             complement(5449..6072)
                     /gene="pth"
                     /locus_tag="PLL28_08395"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNF69379.1"
                     /translation="MKLLRFLFSKKQPTPSTTVEPDMKFLIACLGNIGADYDFTRHNI
                     GFDVADHLAAKHNVSFTSGRYADVAEIRIKGRQLTVIKPTTYMNLSGKAVKYWMDKEK
                     IAIEHLIVVTDDINLPLGTLRIRKKGSDGGHNGLKNITEILGSDQYPRLRFGAGNNFP
                     KGAQVHFVLGKWESEEEADVRTNIERAGNAVISMVLEGIDRAMNTYN"
     gene            complement(6120..6731)
                     /locus_tag="PLL28_08400"
     CDS             complement(6120..6731)
                     /locus_tag="PLL28_08400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNF69380.1"
                     /translation="MQTFQLEGKVRDHVGRRSAMDARAESMVPCVLYGDGENIHFEVA
                     NSAIKNLVYTPNVYKVHVTVNGATHECLMREIQFHKVTDEILHIDFLKLNPAKKVTCV
                     VPVKLEGQSIGVKNGGKLVQRLKKLSIKALPKDLVDKVTLDITDLDINKTARIGDINI
                     ANVEVLGSKSIPIATIASPRALKTAATDDKGAAAAAPAAEKKA"
     gene            complement(6749..7690)
                     /locus_tag="PLL28_08405"
     CDS             complement(6749..7690)
                     /locus_tag="PLL28_08405"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNF69381.1"
                     /translation="MVTNDVKLFSGTTSRYMAEKVADYYGQPLGKMTIDKFSDGEFQP
                     NILESVRGSYVFLIQSTMFPSDNLMELLLMIDAAKRASASYITAVIPYFGLARQDRKD
                     KPRVSIGSKLVAELLTAAGANRIVTMDLHADQIQGFFDIPVDHLSSTAIYVPYIEENL
                     DLTNVIFASPDVGSTKRNRRYAMHFNTELVICDKYRKKANEIAEMTVIGDVSGKDVIM
                     IDDIVDTGNTLANAANLMKQKGAKTVRAFCTHPVLSGKAYETIENSSLEEIVVTDTIP
                     LKRESSKIRVLSSAKLFARAIRNTHEHRSISSLFINN"
     gene            complement(7750..8541)
                     /locus_tag="PLL28_08410"
     CDS             complement(7750..8541)
                     /locus_tag="PLL28_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HNF69382.1"
                     /translation="MIISDIDAFIASGNFPPKRIISLVPSLTELLVDIGLEDALVGVT
                     KFCVHPVGINKRVQVIGGTKNLRVDLIRSLNPDLIIANKEENEQNQVEALASDVPVYL
                     SEITCVADAYEMMRIVGRLTQTTTTVQQRIFELERAEAGLRQQSSISPPKKVLYLIWR
                     DPWMSAGGDTFIDDMLNCCGWENVVAERRRYPSISDTEIRALEPELVLLSSEPYPFKD
                     KHRAEIEALLPNAKVQLADGEAFSWYGTRMHHSFDYLRNLVAKMA"
     gene            complement(8538..9902)
                     /locus_tag="PLL28_08415"
     CDS             complement(8538..9902)
                     /locus_tag="PLL28_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNF69383.1"
                     /translation="MWHKNILDTIGNTPLVQLNNIVKGIDATVLAKVETFNPGNSIKD
                     RMALKMIEDAEKDGRLQTGGTIIEGTSGNTGMGLAIAAIIKGYKCVFTTTDKQSIEKV
                     NALKAFGAEVIVCPTDVDPEDPRSYYSVSSRLVREIPNGWKPNQYDNPSNAKAHYLST
                     GPEIWQQTEGKITHFVAGVGTGGTICGTGRYLKEQNPDIQVFGIDTYGSVFKKYKETG
                     IFDKNEIYPYVTEGIGEDFLPENVDFSVIDRFEKVTDKEAAIMTARITREEGIWVGNS
                     SGSALQGLLQLKDQFKKGDVVVVIFVDHGTRYLGKMFNPSWMMKMGYVDKSGLTARDL
                     ISSNQAGNLISVDAHTTVSQSLQIMMENDFSQLPVTDGGRIVGAINESTLYQQVVKDP
                     ELRMKTVGEVMSAAFPFVDISAPVDALSAMLTPENPALLVRDFKVDKTFIITRYDVMH
                     ALLK"
     gene            10207..>11090
                     /locus_tag="PLL28_08420"
     CDS             10207..>11090
                     /locus_tag="PLL28_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNF69384.1"
                     /translation="MKKFFKWLGILILGMLVIGVIAVVIMINKFSKQSKVEYAVNFTE
                     VALPTDSASLARGAVIAQSTCTSCHGGDFAGTPFFEDEKLGSIPAPNITKGGRTKNYT
                     VKDWQRTLRYGVKPDGHGAFIMPSTEFNHLSDSDLGSLIAYMQTVPASDKQWPDPSMT
                     TLAKILAGAGMFGVMYPASVIDMSDTKPRTNPPPGPTVEYGAYTVAFHGCKTCHGEQL
                     NGYISPDPLSPPGANITPAGNIGKWSLDQFKNTLRTGETPEGKKLDTKFMPWAAMGLM
                     TDMEAEAVFNYLKSMPAL"
     assembly_gap    11091..11190
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11382..11963
                     /locus_tag="PLL28_08425"
     CDS             11382..11963
                     /locus_tag="PLL28_08425"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNF69385.1"
                     /translation="MKLAKFIMIIGILVAGIRLQAQNQYTLGSDHKITINGSSNVHDW
                     EETAQTATGDASVVWNTDGSFVIKSFNLKVSVKSIKSDKGSVMDNKTHDALKADEHPY
                     ITFKMLSVKSMTKSGNGWAVKVNGDLTIAGFTKNIDISGMVYVKENGKLYIDVSRALK
                     MTDFKIDPPTALMGTMQVADDITIRFKLNYNMK"
     gene            11999..13393
                     /locus_tag="PLL28_08430"
     CDS             11999..13393
                     /locus_tag="PLL28_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69386.1"
                     /translation="MRNSSIIRSGLLMAFVLLTGSMFAQYGEINYYRPYDKGGLNVFE
                     TSKESTVPYDGFKLHISGGFTQQYQNLSHSNTATAVLKDNTLTPTTGDSVNINALYPL
                     VGGFNLATANLIIGAQLADGIYVNLENYMSARHHNEFWVKGGYIQIDKLPMFGNPQWF
                     TDNFTVRVGDMEVNYGDAHFYRTDNANGIYNPFVGENIMDAFATEVAGEFYYKPSNGI
                     IAMLGLSGGLLNSDVKVFVDPNTGDTISRKPSIYGKLGYDKKLNDDLRVRLTASLYMN
                     GGTSRNTLYGGDRAGSRYYYAGETSYYMSGSTLTAATATNKAFSGRIDPGFTSKVTSI
                     MINPFVKFHGLEVFATYEMSSGNASLSNTLDSTERSFSQLAASVVYRFLKDEQLYVGA
                     RYNTVSGRLYGYDDTAGDITVNRMQIAAGWFPTENLLLKLEYVNEDYLDFKTDDSRNG
                     LNFKGVMVEASVGF"
BASE COUNT         3846 a         3036 c         2734 g         3962 t
ORIGIN      
        1 tctgaatatg gaaatgaagg cacctgggag aaaattacaa tcatagattt tccttttgat
       61 aattatacag gtaccgttca aacagatgtt gacggtgatt taattactga tactataggc
      121 actactgatg gctcccattt tatgaccata actgatgatg gtaccgtgca tgtattctca
      181 ccctactatc gtatttattc tgatctcgga gcttttttct ggactgtaaa ttggaatacc
      241 atgggattat ggcactggtc ttcaggtatg cctgctgcag aagtgattga tctcgaaatg
      301 gattggatta atgccgattg cagcaacgat ccatatgttg gaattggtgc cagcactttt
      361 aattatcgca atgcagcata cgtcaccgat ccgggtgctg catatgatcc tatcagcggt
      421 cgcatgtttt tattatacac catgaaaatt gaatacacag atgtattcgg tgatccaaca
      481 aacccttccg cacaatcttt tcacgatatt ttcggaacct atagcgatga cggcggtgca
      541 acatgggcgc aacctgttaa tctgaccaat actgcagaaa caggtgaaga aaacttcttt
      601 atgtatgtgt atgatagagt tgttgatgga aaagtatacg ccgtttggca gcaggatggt
      661 gaaccggggc attttaatga aggcgatccg ataacagtaa ataatattaa attccgtgca
      721 tgggatattg aaagtttcaa tccgacattg ccaactgcag cgtatttatt tgtaactgat
      781 attggcgaag taacttttac aaatatttca gaagatgcaa caggttgtaa tttctgggat
      841 tttggtgatg gcactaccag taatgaatct gatcccgtgc atatctatac agtagccggt
      901 gaatatacgg tttgcctcac cgcagaaaat ccatacggca cggatactta ttgtggaact
      961 atttatatca gccttcctcc tgatgcagca ttttcatata ccggcgatcc tatcgtaacc
     1021 ttcaccgatc ttaccttaaa tgaccctact tcatggtcat ggaattttgg tgatgctact
     1081 accagcacat tgcaaaatcc ggtgcatact ttcacttctg aaggaacatt tactgtttgc
     1141 cttacagcaa caaatgcact cggattcgaa gtatattgct tgcctgttac gatagattct
     1201 accgaattac ttgcgccggc agctgattat agttttacag ttgcaggtgg cactacagtg
     1261 aatttcaccg acatgtctac aaatgctcca acgtcatggg catgggattt tggtgatggt
     1321 ggttcttcta ccgaacaaaa tccttctcat gcattccctt catcttcatc ttatgaagtt
     1381 tgtttgattg cagcaaacgc atttggaagt gataccgttt gtaaaacagt attaggcagc
     1441 gaagtgatag acctcagcga tatgattact ttgcaaccga atcctgcaca tgatatggtt
     1501 accatacata ccggaacttt ccatccggat atgatacatg tgtatgatat cacaggtcgc
     1561 gaagtaatgg ttgaagtgca attgtcagct aatgatactt atgtaatgca agtatcatca
     1621 ttgcctcaag gcgcttacat tgtgcacatg caacatgaaa atgaaagtgg ttacgccaga
     1681 ttgatagtgc agtaaccgga aagaatattt tttgtaatgc aggaattacc ttcagaagga
     1741 gagtaattcc tgcattactg tttttacctg ttctacattc ggcaacatcg cacgctccaa
     1801 tcccgaattc aatggaactg caggaaggtc tttagcgccc aatgttctta ccggtgcatc
     1861 aagatagcgg aataattcag aacctattcg tcccgacatt gcctgtgcat atgaattgat
     1921 caatggctct tctgttaaca caaaacattt attatgtcgc ttaaccgatg cggtaattgc
     1981 ttcccagtcg tatggatata atgtgcgcaa atcgagtatt tctacctgac ctggaaattg
     2041 tttcgatgca ttcaacgccc agtgcacgcc catgccgtaa gtgataacca ccatgctttc
     2101 gccattatga atatgcttgt cgtcggcatg ttggaaaatt cttgctttgc caagtggtat
     2161 gatataatca tcggccggtt gaatattttt tgcagctgca gttcccggta ctttactcca
     2221 atacaatccc ttatgttcaa acatgacaac aggattagga tcataaaatg cacctttcaa
     2281 taatcccttc atgtctgcgg cattgctcgg atatacaacc ttaattcctt taatggcagt
     2341 gatagatgat tcaatagaac cgctgtgata aggtccgccc gaaccatacg caccaatagg
     2401 cacgcgaatg atagattgca cattccattt gccattggtg agataatatg atttggataa
     2461 ttcgcttacc aattgattca cgcccgtcca tatataatca gcgaattgaa tttcaacaac
     2521 tggctttgca ccaacagcac tcataccggc agttgaacct actaaataag cttcctgaat
     2581 ggcagtatta aatacgcgat gatcgccatg ttttgcagcc agtgttgccg cttcgcgaaa
     2641 tacacctcca agtcggcgac ctacatcctg accataaaat aatgcttccg gatgtttagt
     2701 aagaatttca tccatggcaa ataatgcagc atcaaccatc ggcactacat cagcaccttg
     2761 tggttcgcgc gtgccttgtt cttcagtgta tggcgaaggt gcaaattcga aatctgatac
     2821 tgttgcagga tcaggatctg ctgatgctaa aactgtttga aaatcagctc tcgtttgttg
     2881 atcggcttca gtttctattg ttgttatttc ttgtaatgaa atgcccgcat caaccaacac
     2941 ctgtcgcagt cgcggcagcg gatcacgtga agcatgttct ttcaaatctt cttcactgcg
     3001 atacatctcc atccgcacac cacttgtatg atgtccgagt aatggcactt ttgcatgaat
     3061 gagtacaggt ttgcgatgca cacgcacata accgatggcg tctttcacac attgccagct
     3121 ttcagcaaaa tcagcaccat caacgcgtac tctgcgcaat cctttaaatc cacctgcata
     3181 ttcataagca tccattgtcc gcgcttcttc tcgcgataca ctgatacccc aatcattatc
     3241 ctgtaccaaa taaataatcg gtaatccttt taataccgaa aattgtaatg cttcagaaac
     3301 ttcaccctct gtcatcgcac catcgccaat agaacaaaca actaaaggat gatcattcga
     3361 agttgccaac aaattttgtt gctctaaata ttgaatgccc tgcgcaacac ctgttgttgg
     3421 aatagcctgc atacctgttg ccgacgactg atgtatcatc tgaggataat cagggccttt
     3481 ataactgggg tgacaataat aatttcttcc tccgctaaat ggatcatcgc gcttggccat
     3541 caattgcaac atgatttgcg ccggctgcca tcccatgccc attaaaaatg catcatcgcg
     3601 ataatataaa cttgcccagt cgctcttttt taattgcatg cccatagcga gttgtatcgc
     3661 ctcgtgacca cgtgaattgg catgcacaaa aacacatacc tgacggtttt cattaaatat
     3721 atcactcatc accttgctgg tgtacatctt gcgatatgcc tccagcaata ccttgcggtc
     3781 gatcatttcc aatgtctctt tttgattcat tatcctattg acgaaatact tatttaatat
     3841 gcgttgtaaa taatacgttg ccgttcaatg atccttcaag cacatcacca atggcaatag
     3901 gtcctacacc tgccggtgtc ccggtgaata ataaatcgcc ctgctgtaaa gtgaaatagt
     3961 tgcttaaata actgatcacc tccggcacgg gaaataacat ttcaccggca ttccctgttt
     4021 gcactgcaac gccatttttc ttcaattcaa aacttgcacg tgaaagcagc gacatatcct
     4081 ccagtggctg catggttccc actacggctg attgatcaaa tgctttcgca atctcccatg
     4141 gcaatccttt cttcttttgc tctgcctgta catctcgtgc tgtaaaatca atgccaacag
     4201 taatgccatc aacatacttg ctcgcaaatt gtggtgcaat atgcttaccg tttttacaga
     4261 tacggaacac taattcgcac tcaaagtgca tatcttttga aaaatcaggc atgtaaaaat
     4321 cgttgccctt cagaatggct gtgggtggtt tcataaagat gaccggagcc tcaggaatcg
     4381 cattattcaa ttccttggca tgctcgccat aattcctgcc aacgcaaaag atcttcataa
     4441 gcggcacaaa attacaaaag cgataccgca ccaacagcag cagtacccca tttcctgtat
     4501 ctttgtatat gcgattttca ataaaacacc tgattttaag cagtttttgc ctcctgatga
     4561 tgctcccctc attgcaggca cagcgccaat tgggctcaaa accaatgggc aacggacaac
     4621 cgggctctgc aggcaaatca ttcgatcata ttgaaggtac ttatgctgtg gttaacggca
     4681 ttaatatgta ttacgaacaa tacggcgaag gggatcccat cctgctgatt cacggcaacg
     4741 gcggctcaac cgaatccttc gacggtcaaa ttgcattcct ctcgagaaaa tataaagtta
     4801 ttgttgctga ctcaagaggg caggggagat caactaataa tgccgatagc ctgacgtatg
     4861 aactgatggc ggaagatttt tatctgttcc tggagcaatt ggaactcgat agtgtaaata
     4921 ttgtgggttg gagcgatggt ggtatcatag gactgatcct tgcccgcgat tatcctgaaa
     4981 aagtaaaaaa acttgttgcc gtaggtgcaa atttgcgtcc cgataccaca gcggtgagtg
     5041 aagcattact cgactggaca cgcatgaaaa tggaacaggc acaggattct gttgcggcag
     5101 gaaaaaaatc attcgccaca gaagaaatgc tcatgcgttt gatgttgcat catcctaata
     5161 ttgatgttgc cgatctgcat gatattaaag cacccgtaat ggtagtcgcc ggcgatcacg
     5221 atctgataaa agttacgcat acagttgaga tctacgaaaa tcttccgcat gctcaattag
     5281 cgatatatcc gggtgcaaca cataatctca tgctggaatc accgcaatta tttaacaacg
     5341 ccgtattgcg atttttctcc agaccttatg ttgctaaaga cccgatgtcg gtgatgttag
     5401 ataccccttc gaaaaataat gatgaagaat aataattaac cttcacggtt aattgtaagt
     5461 attcattgcg cggtctattc cctcaagcac catgctgatt acggcatttc ctgcgcgttc
     5521 aatattggtg cgcacatctg cttcttcctc gctttcccat tttcctaaaa caaaatgtac
     5581 ttgtgcgcct ttggggaaat tatttcctgc accaaatcgc agtcgcggat attgatcgga
     5641 acccaaaatt tctgtgatat tttttaatcc gttatgacct ccatcactgc ctttttttct
     5701 gatgcgcaga gtgcctaacg gcagattaat atcgtcggta acaacaatga gatgttcaat
     5761 agcaatcttc tctttatcca tccagtattt caccgccttg ccactgagat tcatataagt
     5821 ggttggcttt attacggtta gctgtcttcc tttaatgcgt atctccgcaa catcggcata
     5881 tcttcctgat gtaaaggata cattgtgttt cgcagcgaga tgatctgcaa catcgaaacc
     5941 gatattatga cgtgtgaaat cataatcggc accaatattt ccgaggcatg cgataaggaa
     6001 tttcatgtca ggctcaacag tagttgaagg agtgggttgc tttttggaaa ataaaaaacg
     6061 aagcaatttc atgtactgct aagttaacag aaattgcttc gttaagtatg ctgaacggat
     6121 caggctttct tctctgctgc aggagctgct gctgcggcac ctttgtcatc agttgcagct
     6181 gttttcagcg cgcgtggtga tgctatggtg gcaataggaa tgctcttaga accgagaact
     6241 tctacgttgg caatattgat atcgccgata cgcgccgttt tgttgatgtc gagatctgta
     6301 atgtcgagtg taaccttgtc tacgagatct tttggcaatg ctttaatgct cagttttttc
     6361 aaacgttgaa caagcttacc accattttta acaccaatag actgaccttc caatttaaca
     6421 ggtaccacac aggtaacttt tttggcagga tttaatttca gaaaatcgat gtgcaagatc
     6481 tcatcagtca ctttgtgaaa ctgaatttcg cgcatcaagc attcgtgtgt tgcaccgtta
     6541 acggttacgt gaactttgta aacgttaggc gtatacacca aatttttaat cgctgaattg
     6601 gctacttcga aatggatgtt ttcgccatca ccatagagaa cacaaggcac catgctttcc
     6661 gcgcgtgcat ccattgctga acggcgccct acatgatctc tcaccttgcc ttctaattga
     6721 aatgtttgca ttgtgattga gttattgttt agttattaat aaaaagcgaa ctgatacttc
     6781 tgtgctcatg cgtgttgcgt atcgcgcgtg caaataattt tgccgatgac aacaccctga
     6841 ttttcgacga ctctcgtttc aacggaatgg tatccgttac cacaatctct tcaagcgacg
     6901 aattttcaat tgtttcatac gccttgcccg ataataccgg atgcgtacaa aaagcacgta
     6961 ctgtttttgc gcctttctgc ttcatcagat tggcagcatt ggcaagtgtg ttacccgtat
     7021 caacaatatc gtcgatcata ataacatctt tgcccgaaac atcaccgata accgtcatct
     7081 cagcaatctc attggccttc ttgcgatact tgtcgcaaat aaccaattca gtattgaaat
     7141 gcatggcata gcgacgattg cgtttcgtgc tgcctacatc cggtgatgca aaaattacat
     7201 tggtcaaatc gaggttctct tcaatatatg gcacatatat cgccgttgaa ctcaggtgat
     7261 ctaccggtat atcgaaaaaa ccttgaatct gatcggcatg taaatccatc gttacaattc
     7321 tattcgcacc cgcagcagtc aataattccg ccaccaactt agaaccgatg gaaacacgtg
     7381 gcttgtcttt tctatcctga cgcgccaatc cgaaatacgg aattacggca gtgatgtacg
     7441 aagcagaagc gcgttttgca gcatctatca tcaacaataa ttccatcaga ttgtccgaag
     7501 gaaacatagt gctctgaata agaaacacat aacttccacg cacactctcc aaaatattcg
     7561 gctgaaactc accatcactg aacttgtcaa tggtcatctt tcccagcggc tggccatagt
     7621 aatcggcaac tttttccgcc atgtagcggc tggtagtacc ggagaacagc tttacatcat
     7681 tggtaaccat cggactattc gttcgtttga gcccgcaaag ataggaaaaa gccgcgtatg
     7741 aagaaagtgt taagccatct ttgcgactaa atttcgcaga tagtcaaaac tgtgatgcat
     7801 gcgcgttcca taccagctaa atgcttcacc gtcagcaagt tgtactttcg catttggcaa
     7861 taacgcttca atttcggcac gatgtttgtc cttgaaaggg taaggttcgc tcgacaatag
     7921 caccagctcc ggctccagtg cccttatttc cgtgtcgcta atcgaagggt atcgacgcct
     7981 ttcggcaacc acattttccc acccgcagca gttcaacata tcgtctataa acgtgtcgcc
     8041 gcctgccgac atccaagggt cgcgccagat gaggtaaagc acttttttgg ggggagaaat
     8101 tgagctttgt tgtcgcaacc cggcctctgc tctctcaagc tcaaagatcc tttgttgtac
     8161 agttgtggtg gtttgtgtaa gccgaccaac aatccgcatc atctcgtagg catcggcaac
     8221 gcatgtaatt tctgagaggt atacaggcac atctgaagcc agcgcttcaa cctggttttg
     8281 ctcgttctct tccttgttgg ctatgatcaa atccgggttt agggatctga tgaggtctac
     8341 acgcagattt tttgtgccac cgataacctg cacacgttta ttgatgccta caggatgcac
     8401 acaaaatttg gtaacaccaa ccagtgcatc ctccaaaccg atatccacca gcaattccgt
     8461 cagcgaaggc accaaggaaa taattcgctt cggaggaaaa ttgccggaag caataaatgc
     8521 atctatatcg ctgatgatca tttcaacaag gcatgcatca catcatatcg cgtgatgata
     8581 aatgttttgt cgaccttaaa atcgcgcact aataatgccg gattttcggg cgtcaacata
     8641 gcacttaatg catcaacagg tgcagatata tctacaaatg gaaatgctgc actcattacc
     8701 tcgccaactg ttttcatacg caactccgga tcttttacga cctgctgata taaagttgat
     8761 tcattaatag caccaacaat tcttccgcca tccgttacag gtaattgtga aaaatcattt
     8821 tccatcataa tctgcagcga ttgcgaaacg gtagtatgcg catcaacaga aattaaattt
     8881 cctgcctgat tgctgctgat caaatcgcgc gcggtaagac ctgatttatc tacataaccc
     8941 attttcatca tccacgacgg gttaaacatt tttccaagat agcgcgtgcc atggtcaaca
     9001 aatatcacta ctacaacatc gccttttttg aattgatctt ttaattgcag caatccctgt
     9061 aatgcagagc cactcgagtt tccaacccat atgccttctt cgcgtgtaat gcgcgcagtc
     9121 atgatggcgg cctctttatc ggtcactttt tcaaacctgt ctatcacact gaaatctaca
     9181 ttctcaggta aaaaatcttc accaatgcct tctgttacat aaggataaat ttcgttctta
     9241 tcaaaaatac ctgtctcctt atatttttta aataccgagc cataagtatc aataccaaaa
     9301 acttgaatat ctggattttg ctctttcaaa tacctgccgg ttccgcaaat ggtaccaccg
     9361 gtcccaactc ccgcaacaaa atgcgtgatc ttgccttcgg tttgttgcca tatttccgga
     9421 cccgtactta aataatgcgc ttttgcatta ctcggattat catattgatt tggtttccat
     9481 ccattaggaa tttcacgcac caatcgagaa gaaacagaat aataacttct tggatcttca
     9541 ggatcaacat ccgttggaca aacaattact tcagcaccga atgctttaag ggcatttacc
     9601 ttttcaatac tctgcttgtc ggtggtcgta aatacacatt tatatccttt gataattgcc
     9661 gcaatggcaa gtcccattcc agtatttcct gaagtgcctt caataatggt acctcccgtt
     9721 tgcaatctgc cgtccttttc agcatcctca atcatcttca aagccatgcg gtctttgatg
     9781 gaatttccag ggttaaaagt ttcaactttt gccaataccg ttgcatcaat gccctttacg
     9841 atattgttta actgcaccaa tggggtgttt ccgatggtat ctaagatatt tttatgccac
     9901 ataagcgcaa aggtaggaaa aggtgagctt gcctcgccac ggcgggttgt gaatggtgag
     9961 tgtttaaatc gtgatacgtg attcgtgact cgtgactcgt gagtgagatt cctccgcaaa
    10021 ctccgccaac tccgcgattg aaatagggtg agcggtgaat ttctttgcgc ctttgctact
    10081 tagcgtcttt gcgcgcaact cacccttatt tgcaaaatca cccgaaaaaa agtaattttc
    10141 catatcattt gacaaccctc tgccgcggaa taaaggcccg tggtggatac aagcacacca
    10201 ttacacatga aaaaattctt caaatggctg ggtattctta tacttggcat gctggtcatc
    10261 ggcgtgattg ctgttgtgat catgattaac aaattctcta aacagtcgaa ggttgagtat
    10321 gccgtcaact ttaccgaagt tgccttacca actgatagtg cgtcattagc tcgtggtgcg
    10381 gttatcgctc aatcaacctg cacgagttgc cacggcggtg attttgcagg gacaccattt
    10441 ttcgaggatg aaaagttagg aagtatcccg gcaccgaata tcaccaaagg cggacgtact
    10501 aaaaattaca ctgtaaaaga ttggcaacgc acactgcgtt acggcgttaa acctgatgga
    10561 catggtgctt ttatcatgcc aagtacggaa ttcaatcacc tgagtgatag tgatcttggt
    10621 tcattgattg catatatgca gactgttccg gcatctgaca agcaatggcc cgatccttcg
    10681 atgactacct tagcaaagat ccttgccgga gccggaatgt ttggcgtaat gtatcctgca
    10741 tccgttatcg atatgtcaga cactaaacca cgcacaaacc cacctcccgg accaacagtt
    10801 gaatacggag catatacggt ggcgtttcat ggctgcaaaa catgtcacgg cgagcaattg
    10861 aatggataca tttcacctga cccgctttct cccccggggg caaacattac tccggcagga
    10921 aatattggta aatggtcact tgatcaattt aaaaatacac tgcgcacagg tgaaacgcct
    10981 gaagggaaga aactcgatac caaatttatg ccttgggcag cgatgggtct gatgaccgat
    11041 atggaagcag aagcagtatt taattattta aaaagcatgc ctgcacttga nnnnnnnnnn
    11101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tagtaggttt tgacacgtta tatttttaac
    11221 aatatgacca atttgttaga taaaaccttg cacaaaggtt gcgcagatac catcgtcaaa
    11281 agatgacata agtccctttc ggcgaacact attataatca gttgaggttc cgccggtaac
    11341 ttattttgca ttgtcaatta aatgcatcaa aaccttaaga tatgaaactg gcaaaattca
    11401 tcatgatcat cgggatactg gttgccggta taagattaca ggcgcaaaac caatataccc
    11461 tcggttctga tcataagata actattaatg gctcaagcaa tgtacatgac tgggaagaga
    11521 cagcacaaac tgctaccggc gatgcttcag ttgtttggaa taccgatggt tcatttgtga
    11581 tcaaatcttt caatttaaaa gtatcggtta aaagcatcaa aagtgataaa ggttcagtaa
    11641 tggacaataa aacacatgat gcactgaaag ccgatgaaca tccttacatc acctttaaaa
    11701 tgctctcagt aaaatccatg acaaaaagtg gaaatggctg ggcagtaaaa gtgaatggtg
    11761 atttaaccat tgcagggttc accaaaaata ttgatatcag cggaatggtt tacgttaaag
    11821 aaaacggtaa gctgtatatc gatgtgagca gagctttgaa aatgaccgat tttaaaatcg
    11881 atcctccaac agcactgatg ggtaccatgc aagtggcaga cgacatcacc atacgattca
    11941 aattaaatta caacatgaaa taatcatttt tataacctat ctataaaacc tatatattat
    12001 gagaaactca agcatcatta gatccggttt gttaatggcc tttgtcctgt taaccggcag
    12061 tatgttcgcc caatatggcg agatcaatta ttatagacca tatgataaag gtggtcttaa
    12121 cgtatttgaa acaagcaagg aatcaacagt tccttacgat ggattcaaac tgcatatcag
    12181 tggtggattc acacaacaat atcaaaatct ttctcacagc aatacagcaa ctgccgttct
    12241 gaaagataat acccttactc ctacaacagg cgattcagtg aacatcaatg cactgtatcc
    12301 actggttggt ggtttcaacc tggcaactgc aaaccttatc atcggtgcgc aattggcaga
    12361 tggtatttat gtaaaccttg aaaactatat gtctgctcgt caccacaacg aattctgggt
    12421 aaaaggcgga tatatccaaa ttgataaatt gccaatgttc ggaaatccac aatggtttac
    12481 cgacaacttc acagttagag ttggtgatat ggaagtaaac tatggcgatg cacacttcta
    12541 tcgcacagac aatgctaacg gtatttataa tccatttgtt ggcgaaaata taatggatgc
    12601 atttgcaact gaagtggcag gtgaatttta ttacaaacca tcgaatggta ttattgccat
    12661 gttaggtttg tcaggtggat tactgaattc agatgtgaaa gtatttgttg atccaaatac
    12721 cggtgatact atcagccgta aaccatctat ttatggtaaa ttgggttatg acaaaaaatt
    12781 gaatgatgat cttcgcgtac gtcttactgc ttcattatat atgaacggtg gaacttcacg
    12841 caatacatta tatggcggcg accgcgcagg ttctcgctac tattatgcag gtgaaacttc
    12901 atactatatg tcaggatcta cattgactgc agcaactgca accaataaag cattctccgg
    12961 ccgtattgat ccgggattta ctagcaaagt aacaagcatt atgatcaatc catttgtgaa
    13021 attccatgga ttggaagtat tcgctaccta cgaaatgtca agtggtaatg catcattgag
    13081 caatacttta gattcaaccg aacgcagctt cagtcaactt gctgcctcag ttgtttaccg
    13141 cttcctgaaa gatgaacaac tttatgttgg tgctcgttac aatactgttt ccggtcgttt
    13201 gtatggttac gacgacactg caggtgatat tactgttaac cgcatgcaaa ttgctgcagg
    13261 ctggttccca acagaaaatc tgttattgaa gcttgaatat gtaaatgaag attaccttga
    13321 tttcaaaacc gacgattctc gtaatggttt gaatttcaaa ggtgtgatgg ttgaagcttc
    13381 tgtaggattc tgatcaaggg ttttaattgg gttaacccgc attgtttcga cagtgcgggt
    13441 ttttttaatt agcaaatttg ggaattagca gattagcaaa tcgggagtgt gcttgcatgg
    13501 agactctcgt cttagtgagc aagtttgagg gttagcaagt tagcaagtgt ggttaattag
    13561 ccaattagct gattagccga ttagccaatg tggagtgctt ttaacgctgg cgcgttaata
    13621 gggaatgcaa ttatattgct ttgcaatatt ttaattccga agcggaatca gctccctc
//
LOCUS       DAOQHY010000086        13608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2181_length_13608_cov_5.878846, whole genome shotgun sequence.
ACCESSION   DAOQHY010000086 DAOQHY010000000
VERSION     DAOQHY010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13608
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2181_length_13608_cov_5.878846"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1372)
                     /locus_tag="PLL28_08435"
     CDS             complement(<1..1372)
                     /locus_tag="PLL28_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69387.1"
                     /translation="MRIPIFIVLSCSLRMLIGQNWQPFTTSETFSYQSDTAAAIDIGY
                     RVDSFSVAGTDTFYYLNTVIEPLGGDLYLKYGPQFWQKAMRNAGDGLFYFTDPASMAL
                     YTRGAVGDTWEFNFDDHTYATIIAADTQTVLGELDSIKIIETTDGYFITLSKAHGLIR
                     FPVAPGIAPAYCSLSGIRERNLGNFFLGFKDYFDVHVGDVFEYHYSYFFDVWTAKHRN
                     IQYTITAADVSDTLAVFSMEGKQLIAEGIVDPISTLELIDTTIEIRPADHSLTTKLPR
                     AYANMRLTFPFYWYLTESIYDIFQNWGIDSLGNQITGDAYCDIFSPTPYYLFDVDAET
                     AQLYTDYFVSNNFCINYTAGLGLTRVYHYGMESGGSLELTAYVKDGDTVGTLTPDSLY
                     AIAIHESSISKLKLYPNPASEFILISIPQQFETDMRCEVISVAGNRIPITTSRYADKI
                     MADLRSL"
     gene            complement(1789..3690)
                     /gene="dnaK"
                     /locus_tag="PLL28_08440"
     CDS             complement(1789..3690)
                     /gene="dnaK"
                     /locus_tag="PLL28_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNF69388.1"
                     /translation="MGKIIGIDLGTTNSCVSVMEGNEPVVIPNDEGRRTTPSIVAFLE
                     NGERKVGDPAKRQAITNPRKTIQSIKRFMGHRYSEVSNELSMISYEVTRGDNDTARVK
                     IGDRLYTPQEISAMVLQKMKKTAEDYLGTEVTEAVITVPAYFNDSQRQATKEAGEIAG
                     LKVKRIINEPTAAALAYGLDKRHKDMKIAVFDLGGGTFDISILELGEGVFEVKSTNGD
                     THLGGDDFDKKIMDWLADEFKKDEPTVDLRKDPMALQRLKEAAEKAKIELSSSQETEI
                     NLPYITAIDGVPKHLVKKISRSKFEQLSDDLIQRTLRPCEAALRDAGYNKSDIDEVIL
                     VGGSTRIPKIQEVVEQFFGKKPNRGVNPDEVVAVGAAIQGGVLTGEVKDVLLLDVTPL
                     SLGIETLGGVFTRLIESNTTIPSKKSEVFSTAADNQNQVEIHILQGERSMAKDNRTIG
                     RFILDGIPPARRGTPQIEVIFDIDANGILHVTAKDKGTGKEQSIRIEASTGLSDDEIK
                     RMRDEAKANEDADKQSRERIEKLNRADGMIFETEKQLNEYGSKLSDATKAPIEAALNK
                     LREAHKAQDPEGCEVATNELNAAWQNASQEIYAAQQNAGANPDPSAGAADNADNVNKT
                     DDVEFEEVK"
     gene            4003..5400
                     /locus_tag="PLL28_08445"
     CDS             4003..5400
                     /locus_tag="PLL28_08445"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HNF69389.1"
                     /translation="MRKVLFLSLAIVAITTNAAAQSWLTAGNTLTGTEKFGSTNNKSL
                     DIVTNNTVRMKINNKGNIGMGITTLGVNDRLSVKITAPVPTATYTMTRGIFAEVTGNA
                     TATFNSPTVAIYGKSNNNNDWGYGGYFEGDLYGAYGKGKYGFFGYGTGASFTGDDLAV
                     GVKGLAEGDDIINYGVYGTAPSDASAYNYGVAAYAGGSFFGNYALLAVDELGGPGAAG
                     FFDGDIEYTGSIFDVSDRQFKTNIKDLDNALSRINQLQPKSYDMKADEFGGLTFGRND
                     EMGFIAQEVQEVFPELVKSCVSPVDPYTMGGQPSKTEPFEFLAVNYVGLIPVLTKGIQ
                     EQQAMINEQANVIKTQEAVIADLVARVSKLETKQNSNIEAVIDGNNARLEQNTPNPFE
                     GTTAIGYFIPEYTSSAKLIISDMTGKEIKSIDINTIGDGTIQLTAGALSAGNYTYTLV
                     ADGVVVGTKFLSVAK"
     gene            5516..6592
                     /locus_tag="PLL28_08450"
     CDS             5516..6592
                     /locus_tag="PLL28_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69390.1"
                     /translation="MQKWMVAGGLALAALTNSCSTDFDLNADFIETPVIYGLLDASSD
                     TQFIRINRAYLSDEIDGLTMAADPEAIYYGDELEARIEEYSGGTLLHTYILERVDGDT
                     LGLPKADGIFANHPNILYRFIADLDYTHTYKLFAINNETTKSVEATTQLVDSFAIIRP
                     DDESVFPQAISLAPQTVYQLRWRSSQDAKIYDLTMRFHYREGIYYEDGDSVHYTFSGY
                     KDWKMETNYTAESTDGGLILTYDVPGETFYNFIKDSFEPVADNNFIRIADSVQFFIDG
                     GGEELFNYFEYNNATLGITEGQITPAYTNVFGGLGILSSRYHKEGLIYPFAIQTRDSI
                     ACSSVTSGLNFAPDGSTFGFPYCQ"
     gene            complement(6649..7707)
                     /locus_tag="PLL28_08455"
     CDS             complement(6649..7707)
                     /locus_tag="PLL28_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69391.1"
                     /translation="MRRLLGIIAFCILPFFQWRCDNTLQINGNYEEKVVVYGLLNYGQ
                     DTNYIRIQKTFLGENTSALNLASQAGENYYSAGELEVKLEQWKFGALVNTTFCDYVDG
                     DTLGIEKDSGVFASSPNILYRICMPLDSSSTYKLHILRLEDSSIFTAETNLVQSFYLF
                     SPTKPNAYISFADTGNITYTCKQAVNGMIYDLVMTFRYAEVRHINGDSTIHSIDWVIF
                     DNKEGTGTIGISNLNYTLSRHAFYSFIASSLEVDTAVTRYAININYAWYAGGIELYDQ
                     YLNLLANLGLNEDYISPEYTNVNGGLGIFSSRYIEAANNLILDDISLDSLSCGAITRQ
                     LRFVGSASNHDYPACWEQ"
     gene            complement(7826..10210)
                     /locus_tag="PLL28_08460"
     CDS             complement(7826..10210)
                     /locus_tag="PLL28_08460"
                     /inference="COORDINATES: protein motif:HMM:NF019229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HNF69392.1"
                     /translation="MSKKTKRILLITVGVLVLLAGASYAMKGKIKNMVKGKDNKRVAA
                     QQSADIKEVAWDDSKTGNEAMIEYLSKVYDQVNKPGLRFMNTAILNSLLAQPIPQDPV
                     GMYTWYFDVCNQMLNAGKVNEAVEKTKEFEAKPQFAKLSPKELGGWYYTKGLVALRFG
                     EVENCIGLHNSESCIWPLSQAAQSAKKDGPNMAVEAFTKCLQYDKENLSAMWLMNVAY
                     MQLGTYPDAVPSQWLIKPESIKSEYDVPKFKNIAIDLGVDNPNMCGGVILDDFNNDDL
                     IDIFTCGWGLKERSFLLLNKGDGTFDDISTKAGLDRYPGGLMIQQTDYNNDGNLDVWV
                     SRGAWYSNFGILPSSLLRNNGDSTFTDVTREVGIWTTRPSQAATWADFNNDGWLDLFI
                     GNEASRKGDKINDCQLWINEKGKFHNIAKEAGVDVNSFVKGVSSGDYDNDGDPDLYIS
                     ANGFKNYLFRNDTKKGSAKMKFTNVTKEAGVVGPESSFPCWFFDYNNDGWLDIMNFSY
                     SAGISDNDIPAEYMGKPTESDKTALYRNNGDGTFTNVNEETGLAKRTFLVMGSSYGDF
                     DMDGWIDFYVGTGKPDMRSLTPNRMFRNAEGKYFQDVSTSAGIGVLQKGHEISFADLN
                     NDGYPEIFAQMGGAYEASGFYDCLFENPASFGNNWISIDLTGTTTNKCARGARIKITT
                     IENGKERNIYEWVTQGSSFGANSLRQEIGIGKATAIKSIEISWPTSGKKTILKDVPMN
                     QFIAVTEGSEGFTKINLTPFVFQSTPEKSFTHKMMETMNMGNDRYMNMQHDMGH"
     gene            complement(10356..12638)
                     /locus_tag="PLL28_08465"
     CDS             complement(10356..12638)
                     /locus_tag="PLL28_08465"
                     /inference="COORDINATES: protein motif:HMM:NF019229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HNF69393.1"
                     /translation="MRSTSLVLFSSLSLVILFSAGCGKDKKQREFAQNTVVYDANLPS
                     NEAMAKYLAECNEKYFKNTHNRFASTTRALDFESKPAPKEAKKFVEWKKAIGDEWLFA
                     GDTKKSLAAYEEAMKKVQDEGMKGKLVDDIRYMQAVAYMRLGEQLNCIANHVSESCIL
                     PIAQPAWYVEKDPTIKAVAIWEEILKQHPDDLSAKFLLNIAYMNLGKYPDGMPTEYRI
                     DPNEFKSEYPLQRFPNIAANLGVDFITKSGGVVVEDFNNDGLLDIAASGWFLDEQLKI
                     MINNGDGTFTDNTEVSKLKGITGGLDLKCADYNNDGFMDILIPRGAWWNDFGKLPASL
                     LKNNGDGTFTDVTYETGLMKHLRPTQSAVFADFNNDGWLDIYFGNESRRDSEPYYNEL
                     FLSDGKGKFTEVGKEAGVDVICFSKGVVSGDYNNDGWMDIAISSQGTENYLFRNDTGK
                     NGGKVKFTDVTKEAGVSKPIKSFPLAFLDYNNDGWEDLFVCTYDADNCDYDNAAEYFN
                     GTPKGEYSILYKNNGNGTFTNVTKDMNLQVAMTVMGLNYGDLDNDGWLDIYCGTGTPN
                     YTSLVPKRMFRNNGGVGFQDVTTAGGFGHVQKGHGIGFGDVDNDGDQDVYEDMGGGYE
                     GDGFQGAFYENPGNTGNNWITLQLVGTKANQCAIGSKVKITVEEGGKTRDIYQVVSSG
                     ASFGVNSIRLETGLGKATAIKQIEVTWQGPNTKQVFTNVPMNNFVKLTEGVDAFELQT
                     LQPFKFSLDNHSTMDHSKMM"
     gene            12780..13607
                     /gene="panB"
                     /locus_tag="PLL28_08470"
     CDS             12780..13607
                     /gene="panB"
                     /locus_tag="PLL28_08470"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNF69394.1"
                     /translation="MSSAQPDFNKVKRVTVHTLQEMKEHGVKISMLTAYDFSMARILD
                     DAGVDVLLVGDSASNVMAGHETTLPITLDQMIYHAASVVRAIDRCLVVVDLPFGSYQG
                     NSKVALDSTIRIMKESGAHAVKLEGGREVKESIERILSAGIPVMGHLGLTPQSIYKFG
                     TYTVRATEEMEAQKLYEDAQLLEQLGCFSLVLEKIPASLGEKVAAKLHIPVIGIGAGG
                     GVDGQVLVMHDMLGITKEFKPRFLRRYLNLYDDIKKGVSQYIADVKSNDFPNDMEEY"
BASE COUNT         3848 a         2925 c         2699 g         4136 t
ORIGIN      
        1 ggagtgaacg caggtctgcc ataattttat cggcataccg tgaggtggtg atgggaattc
       61 ggtttcctgc gacagaaatc acttcgcaac gcatatcggt ttcaaattgc tgcggtattg
      121 aaatcaaaat gaattcactt gccggattcg ggtaaagttt aagttttgaa atgctgcttt
      181 catgtatggc aattgcatac agactatcgg gagttaatgt tcctaccgta tcaccatcct
      241 tcacgtaggc tgtcaattcc agcgatccgc cactttccat gccatagtga taaacacggg
      301 tgagtcctaa ccctgcagta taattaatgc aaaaattatt tgatacaaaa taatctgtat
      361 acaactgtgc agtttcggca tccacatcaa acaagtaata tggcgttggc gaaaagatat
      421 cgcagtatgc gtcacccgta atttgattgc ctagagaatc tatgccccag ttttgaaaaa
      481 tgtcgtaaat cgattccgtc aaataccagt aaaacggaaa ggtaagccgc atgtttgcat
      541 atgcacgagg aagttttgta gtaagtgaat gatctgccgg tctgatttcg attgttgtgt
      601 ctatcaattc caatgtagaa attggatcaa caatcccttc tgctatcaat tgtttcccct
      661 ccattgaaaa gacagcaagc gtatcagaca catccgcagc tgtgatcgta tattggatat
      721 tccgatgttt tgcagtccag acatcaaaaa aataggaata atgatattca aatacatcgc
      781 cgacatgaac atcaaagtaa tccttaaatc ctaaaaagaa gttccccaag tttctttctc
      841 ttatacccga taatgagcaa taggctggtg caataccggg agcaacagga aatcgaatta
      901 gtccatgtgc ctttgataag gttataaagt atccgtctgt ggtttcgatt attttgattg
      961 aatctaattc acccaaaacc gtttgcgtat cggctgcaat aattgtcgcg taggtatgat
     1021 catcaaaatt aaattcccat gtatcgccaa cagctccccg ggtatataat gccatagatg
     1081 caggatcagt aaaataaaaa agcccgtcac cggcattgcg catagctttc tgccagaatt
     1141 gaggtccgta tttcagatac aaatctccgc caagaggttc gattaccgta tttaaatagt
     1201 aaaaagtatc agttcctgca acgctgaatg aatcgacgcg gtaaccgatg tcaatcgcag
     1261 cagcagtatc gctttggtac gaaaatgttt ctgaggttgt aaagggttgc cagttttgcc
     1321 ctatcaacat ccgcaacgaa caactcaata cgatgaatat ggggatacgc ataattcaaa
     1381 tataacgaaa atttatgagt ttggtcgtta attggtagtg ggtattgagt atcaagtatc
     1441 aggtatcagg tatcaggtat caggtatcaa gtatcagtat caatgcatgt tcgcatcatg
     1501 tacccgattc tctagtacgc acctcactca cgactcacga ttcacgactc acggttggta
     1561 gtgggtattg attatcaagt atcaggtatc agatatcaat gcatgttcgc atcgtgtacc
     1621 cgattctcta gtacgcacct cactcacgac tcacgattca cgactcacga tcggtagtgg
     1681 gacggggcgc cacccgccta tcggcgaggc aagttcacga aaagcatttc ataaaaaaag
     1741 cccatctacc tttcggcaaa tgggcttatg tatactttat gcgaacaatt acttcacttc
     1801 ttcaaattca acatcgtcgg ttttgttgac attgtcggca ttatctgctg ctcctgcaga
     1861 aggatcaggg tttgcgccgg cgttttgttg agcagcgtaa atttcctggc tggcattttg
     1921 ccatgcagca ttcaattcgt tagttgctac ttcacaacct tccggatctt gtgctttatg
     1981 tgcttctcgc agtttattga gagcggcttc gataggtgct ttggtggcat cgcttaattt
     2041 gctgccgtac tcattcaact gcttttcagt ttcgaagatc ataccatcgg cacgattcag
     2101 tttttcgata cgctcgcgtg attgtttatc ggcatcttca ttagcttttg cttcatcgcg
     2161 catgcgtttg atttcatcat cgctcaaacc ggttgatgct tcgatgcgaa tactttgttc
     2221 tttaccggtg cctttatctt tagcagttac atgcaaaata ccattggcat cgatatcgaa
     2281 aatcacttcg atttgtggcg ttccgcgacg tgcaggtggt ataccatcga gaataaatct
     2341 tccgatagta cgattatctt tcgccataga acgttcaccc tgaaggatgt gtatttctac
     2401 ttgattttgg ttgtcagcag ctgtagagaa cacttcgctt ttcttagaag gaatggtagt
     2461 atttgattca atcaatcgtg taaatacacc accaagcgtt tcaattccga gcgacaatgg
     2521 agtaacgtcg agcaacagca catccttcac ttcaccggtc aacacaccac cttgaattgc
     2581 agctccaacg gcaaccactt catcggggtt cacaccgcga ttcggttttt ttccaaagaa
     2641 ttgttctact acttcctgaa tttttggaat acgagtagaa ccacccacga ggatcacttc
     2701 atcgatatca cttttattgt atccggcatc gcgcaatgca gcttcacaag gacgcagtgt
     2761 gcgttgaatc aaatcatcgc tcaactgttc aaatttcgaa cggctgattt ttttcacaag
     2821 gtgtttaggc acgccgtcaa tcgctgtgat atatggtaag ttgatttcag tttcttgtga
     2881 agaagataat tcgatctttg ctttttcagc tgcctctttc aagcgttgta aagccatagg
     2941 gtctttgcgg agatctacag taggctcatc ttttttgaat tcatcagcaa gccagtccat
     3001 gatcttttta tcgaagtcgt cgccaccaag gtgtgtatca ccattggtag atttcacttc
     3061 aaacacgcct tcacctaatt caagaataga gatatcaaaa gtaccaccac caagatcgaa
     3121 tacggcgatc ttcatgtctt tatgacgctt gtctaaaccg taagccaacg ctgcggcagt
     3181 aggttcattg ataatacgtt ttactttcaa accggcgatt tcacctgctt ctttggtagc
     3241 ctgacgctga gagtcgttga agtatgccgg aactgtgata acagcttcag ttacttcagt
     3301 gcccaaataa tcttcagcgg tctttttcat tttctgcaac accatggcag aaatttcctg
     3361 cggagtataa agcctgtcgc caatttttac tcgtgcggta tcattatcac cacgcgttac
     3421 ttcgtagctg atcatgctga gttcattgct cacttcgcta tagcggtgac ccatgaatcg
     3481 cttgatagat tgaattgttt tacgaggatt tgtgatggcc tgacgctttg caggatctcc
     3541 tactttgcgt tcgccattct caagaaatgc aacgatggat ggcgttgttc tgcggccttc
     3601 atcgttcggg ataacaacag gctcgtttcc ttccattaca gatacgcagc tgttagttgt
     3661 tcctaagtct atacctatga tctttcccat gtttatcggt tattttcagt ttattctaag
     3721 cgttcaggtc aatttttata ccatcttgaa ataactgaca gcatgacaga tttggcattg
     3781 aattctaata cattttgtcc aaaacaaggc aaatttactg acgaatttga tttactagaa
     3841 tttacacata ttattgatta tcaatatttt aagtgtatat ttaatgctct gatatagaat
     3901 attcaactaa atgttgtctt gccggacgca taggttgcaa atagctttgt aacttcaatt
     3961 caagcaaacc caaactttaa cccaaaaaca acaaacaaaa ccatgagaaa agtactattc
     4021 ttatcgctgg cgatagtagc catcaccaca aacgctgcag ctcaatcctg gcttacggcc
     4081 ggaaacacgc ttaccgggac tgagaaattc ggctctacca acaacaagtc gctcgacatc
     4141 gttaccaata ataccgtaag gatgaagatc aacaataagg gcaacatcgg aatgggcatc
     4201 actacactgg gtgtgaatga cagattatca gtaaaaatca cagcaccggt tcctaccgca
     4261 acctacacta tgactagggg catttttgct gaggtaacag gtaatgctac tgctacattt
     4321 aatagtccaa ccgttgctat ttatggaaaa tcgaataata ataacgactg gggatatggc
     4381 ggctattttg aaggtgatct gtatggagcc tatggcaagg ggaaatatgg tttttttgga
     4441 tatggtacgg gagcttcttt tacaggcgat gatttagctg taggtgtaaa agggcttgcg
     4501 gaaggtgatg atatcattaa ctatggcgtt tatggcacag ccccaagtga tgcctctgca
     4561 tataattatg gcgttgcagc ttatgccgga ggatcattct ttggtaatta cgccctctta
     4621 gctgttgatg aacttggtgg tccgggtgcg gcgggatttt ttgatggaga tattgaatac
     4681 accggttcta tttttgatgt gagtgacagg caattcaaaa ctaatattaa agatttagat
     4741 aatgccctga gcagaatcaa ccaattgcag ccgaaatctt atgatatgaa ggccgacgaa
     4801 tttggaggtt tgacatttgg tagaaatgat gaaatgggat ttatagctca agaagtgcag
     4861 gaggtatttc ctgaattggt aaaatcatgc gtatctccgg ttgatccata cactatggga
     4921 ggacaaccca gtaaaactga gccatttgag ttcctggcgg tgaattacgt tggcttgata
     4981 cctgtgctta caaaaggtat tcaagagcaa caggcgatga taaacgaaca ggctaatgta
     5041 atcaaaacgc aagaagctgt aatcgccgac ctcgttgcac gtgttagcaa gcttgaaacg
     5101 aagcaaaaca gcaatattga ggcggtgatt gatggcaata atgcccgttt agagcaaaat
     5161 actcctaacc catttgaagg cacaactgcg atcggttact ttattcctga gtacacatca
     5221 tctgccaaac tgatcatttc tgatatgaca ggcaaggaga tcaaatcgat tgatatcaat
     5281 actattggcg acggtaccat acaattgact gccggtgcgc tttctgcagg taactacacc
     5341 tatacgctgg tggctgacgg cgtagtagtt ggcactaagt ttttgagtgt tgccaaataa
     5401 tggcgcctgg tatataggat aaaaggctct gcatggctgc agggcctttt ttaatagcat
     5461 gattcctgac agagggctta caatcccttc tccaaatcag ctatctttgc gttctatgca
     5521 aaagtggatg gtagcgggag gcttggcatt agccgccctt acaaacagtt gttcaacaga
     5581 ttttgacctt aatgcagatt ttattgaaac gcctgtgatt tatggtttat tggatgcgtc
     5641 ttccgatact caatttattc gcatcaaccg cgcctatttg agcgatgaga tcgacggtct
     5701 tacaatggct gctgatcctg aggctatcta ttatggcgat gaattagaag caagaatcga
     5761 ggaatactcg ggcggaacat tattgcatac ttacattctt gaacgtgtag atggcgatac
     5821 cttaggcttg ccgaaggcag atggcatttt cgccaatcat ccgaatattt tgtatcgttt
     5881 tattgcagat ttggattata cccataccta caaattattt gcaatcaata atgaaacaac
     5941 aaaatctgta gaagctacta cacagttggt cgacagcttt gcaatcattc gtccggacga
     6001 tgaaagtgtt tttccacaag caatttcatt ggcacctcaa accgtatacc agttgcgctg
     6061 gagatcatca caagatgcaa aaatctatga ccttaccatg cgctttcatt atcgtgaagg
     6121 tatttactac gaggacggtg atagcgttca ttatacattc tcagggtata aggattggaa
     6181 aatggaaacg aattacaccg ctgaatctac tgatggcgga ttaattctta cttatgatgt
     6241 tccgggagaa acattttaca atttcattaa agacagtttc gaacctgtag ccgacaataa
     6301 ttttattcgc attgcagatt ctgtgcaatt tttcattgac ggcggtggcg aagagttgtt
     6361 caattacttc gaatataaca atgctacact tggcattact gaaggtcaaa taacaccggc
     6421 gtatacaaat gtattcggtg gtcttggaat attgagcagc cgctatcata aagaaggact
     6481 aatttatcct tttgcaattc aaacaagaga ttcaattgcc tgtagtagtg ttacaagcgg
     6541 actcaatttt gcaccggacg gcagcacttt cgggtttcct tattgccagt gattgggttg
     6601 attggctaat tgccataaat gctgaaaacc aattcgcgct tcacaagatc actgttccca
     6661 acaggccgga taatcatgat ttgaggcact tcctacaaat ctcaattgac gtgtaatcgc
     6721 tccgcacgac agtgaatcca gcgatatatc atctaatatg aggttgttag ccgcttcaat
     6781 atatcgcgaa gaaaaaatac caagaccacc attgacattg gtgtattcag gtgagatata
     6841 atcttcgttc aaaccaaggt ttgccaatag attcaaatac tggtcatata attcaattcc
     6901 tcctgcatac caggcataat tgatatttat agcatatcgg gtgacggcgg tatcaacttc
     6961 taaagaactc gcaatgaacg aataaaaagc atgtcgactc aatgtataat ttaaattact
     7021 aatacctatg gttcccgttc cttccttgtt atcaaaaatc acccagtcaa tggaatgaat
     7081 tgtagaatca ccatttatgt gacgcacttc tgcatatctg aaagtcatca ccaggtcata
     7141 gatcatgccg ttaacagctt gcttacaggt gtacgtgata ttgcctgtat cagcaaaact
     7201 gatgtatgca ttcggctttg ttggagagaa taaataaaaa ctttgaacga gattggtttc
     7261 ggcagtgaaa attgagctgt cctctagtcg caaaatgtgc aatttgtaag tagaagagct
     7321 atcgagaggc atgcaaatcc ggtataatat atttggcgaa gaagcgaata ctccggagtc
     7381 cttttcaatg cctaaagtat ctccatcaac atagtcgcaa aatgttgtat ttaccagtgc
     7441 accaaatttc cattgttcca gttttacttc taattcaccg gcactgtaat aattctcccc
     7501 tgcctgagat gcaaggttaa gagcacttgt attttcacca agaaacgttt tttgtatgcg
     7561 gatataattg gtatcctgac cataattcaa taaaccgtaa acaacaactt tctcttcata
     7621 atttccattg atctgcagtg tgttatcgca acgccattgg aagaatggca ggatacaaaa
     7681 ggctatgatc ccaagcaggc gtcgcatatt caaaagtaat attaattgta attgattgcc
     7741 accttcggcg aaaaaattac gcgaataaaa caaaaacgcc cgtcaattta gacaggcgtt
     7801 ttcaatatgc taaactgtat agaaatcaat gtcccatgtc gtgctgcata ttcatgtagc
     7861 ggtcgttacc catgttcata gtctccatca ttttgtgtgt aaatgatttt tcaggagtgc
     7921 tttggaatac aaaaggtgtg agattgattt tcgtaaatcc ttctgaacct tcagtaacag
     7981 caataaattg attcattggt acatctttca aaatcgtttt tttaccgctg gttggccacg
     8041 agatctctat agatttgatg gctgtagcct taccgatacc tatttcctga cgaaggctgt
     8101 ttgcgccaaa gctggagcct tgcgttaccc attcgtagat attgcgttct ttgccatttt
     8161 caatagttgt gattttaatt cgtgcaccgc gagcgcattt atttgttgtt gttccggtca
     8221 aatcaattga aatccagtta ttgccaaacg atgccgggtt ttcaaataag caatcataga
     8281 aacctgatgc ttcataagcg ccgcccattt gtgcaaaaat ctccggataa ccgtcattgt
     8341 tcaaatcggc aaaagatatc tcatgtccct tttgcaaaac accaattcct gcagatgtag
     8401 atacatcctg gaagtatttt ccttcagcat tgcggaacat acgatttggt gtaagcgaac
     8461 gcatatccgg tttaccggta ccaacataga aatcgatcca tccgtccata tcaaaatcac
     8521 catagcttga tcccattacc aggaaggtgc gttttgcaag gcctgtttct tcattcacgt
     8581 tagtaaacgt gccatcacca ttattgcggt agagggcagt tttatcactt tcagttggtt
     8641 tgcccatata ttcagccgga atatcattat ctgaaatgcc tgcgctatag ctaaagttca
     8701 taatatccaa ccaaccatca ttgttataat caaaaaacca acatgggaag cttgattcag
     8761 gacctacaac accggcttct tttgtaacat tggtaaattt catttttgca ctaccctttt
     8821 tagtgtcgtt gcggaaaagg tagtttttaa agccatttgc tgatatatac aaatcaggat
     8881 cgccatcatt atcataatca ccgctgctca ctccttttac aaatgaattt acatctacac
     8941 ctgcttcttt ggcgatgttg tggaatttgc ctttttcgtt gatccataat tggcagtcgt
     9001 taattttatc tccttttcgc gatgcctcat ttccgataaa taaatccaac caaccatcat
     9061 tattaaaatc agcccatgtt gctgcttgag aaggacgtgt tgtccaaata ccaacttcac
     9121 gggttacatc agtaaatgta gagtctccat tattacgtaa taatgaactt ggcaagatac
     9181 caaaattgct gtaccaggcg ccgcgtgaaa cccaaacatc gaggttacca tcattgttat
     9241 aatcggtttg ttggatcatc aatcctcccg gatatctatc caaacctgct tttgtagaaa
     9301 tatcatcaaa agtgccatca cctttattca ataaaaggaa gcttctttct ttcagacccc
     9361 atccacaagt gaatatatct ataagatcgt cgttattgaa atcgtcgaga ataacgccac
     9421 cgcacatatt tggattatcc acgccgaggt cgatagcaat gtttttaaat ttaggaacgt
     9481 catattctga tttaattgac tccggtttta tcaaccattg agatggcact gcatccggat
     9541 aagtacccaa ttgcatataa gcaacattca tcagccacat tgcagacaaa ttctccttat
     9601 catattgcag acattttgtg aaggcttcaa ctgccatatt cggaccatcc ttttttgcac
     9661 tttgggcagc ttgtgaaagt ggccaaatac aagattcaga attatgcaag cctatacaat
     9721 tttctacttc accaaatcgt aaagcaacaa gtccttttgt ataataccaa ccacccaact
     9781 cttttggtga aagtttagcg aattgtggtt ttgcttcaaa ttcttttgtt ttttcaacag
     9841 cctcattcac ttttccggca ttcagcatct ggttacaaac atcgaaatac caggtgtaca
     9901 tacctaccgg atcttgagga ataggctgag ccaataaact gtttagaatg gctgtattca
     9961 taaagcgcaa acccggctta tttacctgat cataaacttt tgacaggtat tcaatcatgg
    10021 cttcgttacc tgttttagaa tcatcccatg caacttcctt aatgtcggca gattgttggg
    10081 cagcaacgcg tttgttgtct tttcctttaa ccatattctt gatcttacct ttcatggcgt
    10141 atgatgctcc tgcaagtagt accaaaacac caacagtgat caatagaata cgttttgtct
    10201 ttttgctcat aaaaattgaa atattgaggt taagtgtgat ggtgcaaagt tctgatttta
    10261 gcgggcacca atcgcttatt agccgccggt ttgctcaaaa attttgtaaa cggtattaaa
    10321 atatttcttt aacagtttaa ctcaatttca gggatttaca tcattttcga gtgatccatt
    10381 gtagagtgat tatccaagct gaacttgaag ggctgtaagg tttgcaactc aaaagcatcc
    10441 actccttcgg tcagttttac gaagttattc ataggtacat tggtaaatac ctgttttgta
    10501 ttaggtcctt gccaggtcac ctcgatctgc ttaattgccg ttgccttgcc taaaccggtt
    10561 tccaaacgta tggagtttac gccaaaactt gcgccggaag atactacctg atatatatcc
    10621 ctggttttgc ctccttcttc aacggtaatc ttaacttttg agcctatggc acattgattg
    10681 gcttttgtcc ccactagctg taatgttatc cagttattcc ctgtattgcc cgggttctca
    10741 taaaatgctc cctggaaacc atcaccttca taaccaccgc ccatgtcttc ataaacatcc
    10801 tgatcgccgt cattatctac atcgccgaaa ccgataccat gtcctttttg tacgtgacca
    10861 aaacctccag cagttgttac atcctgaaat ccaaccccac cattatttcg aaacatccgt
    10921 ttaggtacaa gtgaagtgta atttggagtt ccggtgccac aatatatatc caaccaacca
    10981 tcattatcta aatcgccgta attcaatccc attactgtca tggctacttg caaattcata
    11041 tcctttgtga catttgtaaa tgtgccattg ccattatttt tgtaaaggat agaatattcg
    11101 cctttaggtg tgccgttgaa atattctgcc gcgttgtcgt aatcgcaatt atctgcatca
    11161 taagtgcaca caaataaatc ttcccagcca tcattattat aatctaaaaa tgcgagcggg
    11221 aagcttttaa taggtttact cacaccggct tctttagtta catcagtaaa tttaacctta
    11281 ccgccatttt tgccggtatc gtttctaaat aaataatttt ctgtaccctg acttgaaatg
    11341 gcaatatcca tccagccatc attattgtaa tcgccggaca ctactccttt ggaaaaacaa
    11401 attacatcaa ctcctgcttc ttttccgact tctgtaaatt tacccttccc gtcactaagg
    11461 aataattcat tgtaatacgg ttctgaatct cgtcgggatt cgttgccgaa ataaatatcc
    11521 agccaaccgt cattattgaa atcggcaaac actgcagact gcgtaggacg aagatgtttc
    11581 atcaatcctg tttcatatgt cacatccgtg aaagtgccgt cgccattgtt tttcagcaga
    11641 gaagcgggta attttccgaa atcgttccac caagcgccac gaggaatcag gatgtccatg
    11701 aatccatcat tgttataatc cgcacactta agatcaagtc cgccggtaat gccttttagt
    11761 ttacttactt cagtattatc tgtaaaagtg ccatcaccgt tattgatcat tattttaagt
    11821 tgctcgtcaa ggaaccaacc tgaagcagca atatctaata aaccgtcatt attaaaatct
    11881 tcaacaacaa caccacctga tttggtaata aaatctactc cgaggtttgc agcaatattc
    11941 gggaatcgct gcaatggata ttccgattta aattcattcg ggtcgatgcg atattctgtt
    12001 ggcatgccat cgggatattt tcccaaattc atataggcga tattcaacag aaattttgca
    12061 gataaatcat cagggtgttg tttaagaatt tcttcccaaa tggcaaccgc tttaatggta
    12121 ggatcttttt caacatacca agccggctgt gcgataggta aaatacaaga ttcagatacg
    12181 tgattggcaa tgcaatttaa ttgctctcca agacgcatat aagcaactgc ttgcatataa
    12241 cgaatatcat ctacaagctt acctttcatg ccttcatcct gcactttttt catagcctct
    12301 tcataagcgg caagcgattt ttttgtgtcg cctgcaaaca accattcatc accaattgct
    12361 tttttccatt caacaaattt tttcgcttct ttcggcgcag gtttgctttc aaaatccaat
    12421 gcgcgtgttg tgcttgcaaa gcgattatgt gtattcttaa aatacttctc gttgcattca
    12481 gccaggtatt tggccattgc ctcatttgaa gggagatttg catcataaac aaccgtattc
    12541 tgagcaaatt ctcgttgctt tttgtcttta ccgcatccgg cagaaaatag tatgactagt
    12601 gacaggctgc taaacaatac taaggaggtg ctgcgcataa ggggtgaaat tttgtgcaaa
    12661 ggtactgtgt tttaaccctg gaagggcatg tttaagaaaa gttttaatgt gcgaaacccc
    12721 ctgatacaca aaggttcatg caaaatatgg tgtatctttc ccccgaaaaa tctagtgtca
    12781 tgtcgtctgc ccagcctgat ttcaacaaag taaagcgtgt aaccgtccac acccttcagg
    12841 aaatgaagga gcacggggtg aagatttcta tgcttacagc ctacgacttc tccatggctc
    12901 gcatccttga tgatgccggg gttgacgtgc ttttagtggg cgacagtgct tctaacgtta
    12961 tggccggtca cgagactacc ctgcccatca ccttggacca gatgatttac catgctgcca
    13021 gcgtagtgcg tgccattgac aggtgtctgg tggttgtaga cctgccattc ggctcctatc
    13081 aagggaattc aaaagtggcg cttgattcaa ccatccgcat tatgaaagaa tcaggggcgc
    13141 atgcagtgaa actggaaggt ggacgtgagg tgaaagagtc tatcgaaagg atactctctg
    13201 ccggtattcc tgtaatgggt caccttggac ttacaccgca atccatctac aaattcggga
    13261 catatacggt tagggctact gaagaaatgg aagctcaaaa actgtatgaa gatgcgcagt
    13321 tactggagca attgggctgt ttctctcttg tattagaaaa aatccctgcc tcccttggtg
    13381 aaaaagttgc agccaaactt catatcccgg ttataggaat tggcgctggc ggtggtgttg
    13441 acggtcaggt tttagtaatg cacgatatgc tcggtatcac caaagaattc aaaccccgat
    13501 ttttacgcag atacctcaat ctatatgacg atatcaaaaa aggtgtcagc caatatatcg
    13561 ccgatgtgaa gtcgaatgat tttcctaatg atatggagga gtattgag
//
LOCUS       DAOQHY010000087        13529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2202_length_13529_cov_5.655262, whole genome shotgun sequence.
ACCESSION   DAOQHY010000087 DAOQHY010000000
VERSION     DAOQHY010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13529
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2202_length_13529_cov_5.655262"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..4749)
                     /locus_tag="PLL28_08475"
     CDS             complement(136..4749)
                     /locus_tag="PLL28_08475"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNF69395.1"
                     /translation="MTTVLLLFLTIFLLLRIPAVQNWVAEKTTSWLSEKLDTEVSVKE
                     VSIDLLDHLILEGLYIEDKQDDTLLYAGKLEVNIGTINPLVPYVKIKSIGLYDTRVYL
                     DRTLPDSIYNFDFISEAFSSNDNKDTSTSSSGGGRSVQLKLNRLDLKNVHFVLHDEVA
                     ASDMQMHVGDLELLINTLDLEDHIIALEKLQIDDTRFALTGLTDTIPSNTPESWDTVH
                     IAMGDWVMEASQLKLHNCAFAFLDVNDTTPNRGINFSDMDITGIDLEMTDIIYHGDTI
                     MNRIDHLALTEKSGFRVDTLHANVLFSPYEISLTDLLIKTPNSKIQKSFAMTFTTLND
                     FDRFESDVRMDGNFDKTIVGNKDLAYFAPVLDKYDAHLLLSGHIYGALDNLKGRDFDA
                     MLNESAGLRGTLNVKGLPNIDETFIDMELEPLYVNTYRLENIIGAGAIPDNLLALGTI
                     NYTGRVTGFAYDLVTLGNLTSDQGNMYTDVHFQYDPDLGKSSFRGDLNTSAFNVGIIA
                     GEPDLLGRISMHATIDGSIARDNEMEMDMQSEISMMEFNDYTYSNMVVDGNLENDYFS
                     GNFTIDDPNVKLTFNGIINMQDSIPLYDFKANIARANLKQLNFYPEPIVFSATASADA
                     GGTSLDNIEGNLHFGDLLLIRDKYIYRLDTLNFTAAQIGDKKSIKANSTLIDLQLEGN
                     FLPSKLDESIKSLVDYYTNGRIDSTLEVQKANYTVSIKNADRLMAIFYPDIQEVRNLT
                     LSGDFNTATRSFNNRLRIDQFNYQNITLDTLLFEARTANDALEYFTRINATHIGSAVV
                     LPVLRSEGNFAQNTLNYNFKAGKDTDSNRVNLNGAVAFIDSSIMFNILPSEIFFEAEK
                     WDILPNNSLKYDNKNLYAQNFTLNSGNKVISLSSSADATYGTVLKLGIRNIPIGELAE
                     RFVLPGEDISGTLDATYTIGNALKDPSFLGGAEIKDLTLNHVLLGNLKVNTSLIQPNN
                     VLKFNSTLSGENGFVTDGYYIMGRGEDEDSLQLKADFRHTKLFIVEPFLRGILSDMSG
                     DIYGTINIDGPVSTPQMQGSLQVKNGGMTVDYMGTHYAFNQIDINVEKNKITIPETTI
                     TDKLNNTASLKGEVTYSHFDNWNFKELHVISDNLVLMETNAKQNPDFYGYAIGEVDCN
                     ITGKLDALLITVDATPHSGTVVNLPTYGSGNVKKHDFIRFVNKQIINTIETADELNLA
                     IVDVALKLNATPDAQIKLLINSEGTEFLSGRGFGALTINANSLGKVDMVGTYRITEGL
                     YDFSFQGFVPRPFTVIPGSTIEFSGSPYKAQLDLTAEYTAENVSINTLTGTDTKETAN
                     VNVLINIDGVLESPEISFDLEVADGNAKNNADFERRINEIKSDKNELNKQVFGLLIME
                     GFLPQDLTAFNAVGTTASSTMNDFFSSQLTTYFQNVLDDFIKDTEIDFGIDNIQSGNY
                     NVTTDLGNKLQLDIKREVTDNLIVKVGTTYYDFTGNSSGNANNLAGDFEVEYIISPDG
                     RVRVKAFRISEYDAIVAKNDVKTGVGIYYSKDFNKLKELFKKQ"
     gene            4861..5874
                     /gene="tsaD"
                     /locus_tag="PLL28_08480"
     CDS             4861..5874
                     /gene="tsaD"
                     /locus_tag="PLL28_08480"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNF69396.1"
                     /translation="MPVTILGIESSCDETAAAVLINGQIMSNVIAGQSVHEQYGGVVP
                     ELASRAHQANIVPVVTVALSRAGISLADLDAVAFTQGPGLLGALMVGTAYAKALAISL
                     DIPLITVHHMQAHILAHLIEDPKPAFPFLCLTVSGGHTQIVRVNSAMDMAILGQTIDD
                     AAGEAFDKCAKMLGLPYPGGPLIDKYAAQGNAQAFDFGEPKIQGLDFSFSGFKTSVLY
                     FLQEQTKKNPDFVKNNLNDLCASVQRGIVEILLRKVKRAAKETGISRIAISGGVSANT
                     ALRKNLTDMGVALGWEVFIPKFEYCTDNAAMIAITGYQKFLAGAFARYDVVPMARIPG
                     IYI"
     gene            5891..6310
                     /locus_tag="PLL28_08485"
     CDS             5891..6310
                     /locus_tag="PLL28_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNF69397.1"
                     /translation="MARIQLTFPEKTLFTTVLDVRITDLNYGGHLGNDSVLSLLHEAR
                     VRFLQYLGFRELDVAGTGIIMSDAAIQFKGEAFYGEQLTIAMAVADFSRVSFDLYYLV
                     TNNDKTIALAKTGIVFFDYTQRKMVPVPEAFLLAVNR"
     gene            6490..10245
                     /locus_tag="PLL28_08490"
     CDS             6490..10245
                     /locus_tag="PLL28_08490"
                     /inference="COORDINATES: protein motif:HMM:NF021665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNF69398.1"
                     /translation="MKLFYSVALCLLGLFSFPITISAQMMDDCAAHPDDDVMHTEHCA
                     VFALVPEDAATHVSIANGSWFSPATWNTGTVPGNEARVLVDSGFTVTYDAVATAAIEW
                     IRVNGTLNFSTTVNTQLRVTDFIIDPLGHLNIGTVAAPIPNGVTAKILFSDLGPIDYM
                     DDPYRFGKGLISHGFLNVNGYYKKTFCAVTKNLNAGTNNIKLAETPTGWQIGDQLVVP
                     GTYGTAYGDFDANTKFHDEVVTITSIAGKTIYFTNNATGTSILQYEHKLPAGYGLKMY
                     VANLTRNVVFESENYNVIPDDQRGHVMLMHNIGASVMNAQFNGLGRTNKDILATDPVV
                     DEFGNLISGGENVRGRYALHVHRAGTNNIAAIPVQIKGCSVVAATSWGVVNHESNVNA
                     DDNVVFDYFGGAFVTEDGNELGTFNRNIAIKGRKATTVTDLTTRTLNIDFAFEGNGFW
                     IQSSNVGYENNIVTSSAGDAYKVFSDDASMPAEYRVKIPKENILNPIIAGADDSIYTA
                     VVPLRKFSGNIAYNCNSAMMFWTHMLNNDNVGDFSTIAYDPYTHTIMSVIDGCKFWNM
                     LGAGISVKYSGQVHFKNTILLGDLTNQYQTSDWIAGNPYGGYAFITSTVTGQIIYEGL
                     TVKGWKRGVVAGRTDDLQSGDTKEYDYRTSKIIGGTYAANLYNIVPEEGTDVYGASEY
                     YKFPDYFEISGSPSFTAITPNVLPTPDFAYLSAGGNAVQFNGALSLDTDPGVITPGQG
                     NGIASYVWNFGDGSSGYGKDPVHTYNLPGTYSVTLTVYDSQGKTGVLTRNVYVSTTQY
                     SNAVANSGFETGGLTTASYINSTRAFVDNGWIHKSNWQLLAGKATILLSDKWNRPLVQ
                     VIKNDKVLRGNVSFSFQAKNLGIGAIGNDLYAEIVGVNGEFKDMNFTSLGAVTKWNLN
                     DVDVVPTVLYSENLGLAEYNWQTFTRTVNFGSGYDYIIVKFYSQGVKTGPGESQGIDN
                     VCLPCVCAVPGGLFADALTSSHATLIWDNVGSTQYQVQYKTTAGATWTTVTVENTYLD
                     LSSLSANTSYTWKVKAWCSGAWTAFSGDNIFITPSAGTTCTSPSELSTELITPNKATL
                     VWNAIPGALQYQVSYKSLLGLTWTDVTTTNTTLSLTGLLPLTAYNWKVKTQCAAGWKD
                     YTTLLTFTTLPLREGEEIGENDLNIQAWPNPVSYELQLTFSEEISGTGSLRLIDISGQ
                     IVKAIDIQIDGTNIAIPVNVSDLPTGIYVLQVALINGQMFNTKIIKE"
     gene            10339..10824
                     /locus_tag="PLL28_08495"
     CDS             10339..10824
                     /locus_tag="PLL28_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69399.1"
                     /translation="MHTRIFTFMILCAMATAVKAQNNELHAVGIRFGGGDGVGAEISF
                     QQYIGGPNRLEFDLGAFEDGYNDGFKLTVLYQWVHSIDNSFYWYAGLGGGLGARDHDN
                     GHPFEGDDDDGVFVDFDFDLGMEYNFDFPLQLSLDIRPEIGLINDDFDMGWALGVRYR
                     F"
     gene            complement(10957..12477)
                     /locus_tag="PLL28_08500"
     CDS             complement(10957..12477)
                     /locus_tag="PLL28_08500"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNF69400.1"
                     /translation="MHIRTTLLALGFASVAMAQNPGDNIFNTNQVLTIDLQFSQPGFW
                     DSLVANYATETYMMADLTVTDNTGTYTFDSVGVRLKGNSSYGHPGNKKSFKIDFNRYV
                     AGQEYDGLRKLNFNNSFKDPSFMREKIYYDICKADSIHAPRANYADVYMNDELWGFYS
                     VVEQVDDEFLKTHFDENNENLFKAGDAFGGGPGGGVEGDLKYYGTVQDDYYDRYTLEN
                     NTDENDWTDLINLTDFINNSSDADFAGQLSHHFDVEQLMKCMVMYNMFSNLDSYLNSA
                     RNYYIYDYDSTGIWEWINWDCNESFGLYTGGPGTGDLQELDIYYYNLNRPLFERVLTI
                     EDTKTQYETYYCELFNAYFNNSYISDLIDELYTLIQPHVYADPNKMYTNDQFETNIES
                     DIAGGMGGTTYGLKSFVTNRSAYIASALDCSTQTDISTNTAFDIHIFPNPTSDMIYIN
                     GDFTSPCKLAVYGLDGRVLYQLDQVQLPTSISLANQPSGMYLVVVGEQQTIITKQQ"
     gene            complement(12540..>13529)
                     /locus_tag="PLL28_08505"
     CDS             complement(12540..>13529)
                     /locus_tag="PLL28_08505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HNF69401.1"
                     /translation="HHFQPADTIQWFEDRGVELHTEVDGRMFPVTNSSQTIIDCFLRE
                     ATKYHVHIHFGVQVESITSVDNAWKIDCKNQTFHAIAVMMATGSSQQAWSLLKQLGHT
                     IIPPVPSLFTFHIRDKRIDGLMGVSIPHVQASLMNTDLITEGPLLITHWGLSGPAILK
                     LSAWGADVLAKKQYRASIQINFCPTYDSASCIDYLRGMRNDHPKKKVVNDHFPTIPAR
                     LWKQLCAFGNITENHNWADLSNIMIESFAEILTSSTFSINGKSTFKEEFVTCGGIALN
                     EVNMRNMESRLHPNLYFGGEMLNIDAVTGGFNFQAAWTTGWLAARSIAGNQTS"
BASE COUNT         3792 a         2612 c         2954 g         4171 t
ORIGIN      
        1 cacaattaga gtgtgaaagt cccgcttagg cgggatgtgg ctgcgctcca tgtggtaatg
       61 tgctagtgag caaagtcagg cgagttaaac gctcaaggtg cctttcaatt tacataatca
      121 gagctgtgtg agagcttatt gctttttaaa cagctctttt agtttgttga agtctttaga
      181 ataataaata cccacacctg ttttaacgtc gtttttagca acgatggcat cgtattcaga
      241 aatacggaat gcttttacac gaacgcgacc atcgggcgaa ataatatatt ctacttcaaa
      301 gtcaccggca aggttattgg cattacctga agagtttcct gtaaaatcat aataggttgt
      361 tcctactttt acaatgaggt tatcagttac ctcgcgttta atatccaatt gtaatttatt
      421 accaaggtcg gtggttacgt tataattacc actctgaata ttatcgatac caaaatctat
      481 ctctgtgtct ttgatgaaat catccagcac attctggaaa taggtagtaa gctgactgga
      541 gaagaagtcg ttcatggtac tgctggcagt agtaccaact gcgttgaagg ctgtcagatc
      601 ttgtggcaga aatccctcca tgataagtag accgaacacc tgtttattta attcattctt
      661 atcgcttttg atttcattga ttctgcgttc gaaatcggca ttattttttg cattgccgtc
      721 ggcgacttcc agatcaaatg aaatttcagg tgattccagt acgccatcga tattgatgag
      781 cacattgacg tttgcggttt ctttggtatc tgttccggtg agggtgttaa tgctcacgtt
      841 ttcggcggta tattcagcag tgagatctag ttgcgctttg taagggctgc cactgaattc
      901 tattgtgctt cccggaatca cagtaaatgg tcgcggaaca aatccctgga agctgaaatc
      961 atataaacct tctgtaatac gatatgtgcc taccatatct acttttccta aagaattggc
     1021 gttgatggta agtgcaccaa aacctcttcc agacaaaaat tccgttcctt cactattgat
     1081 gagtaattta atttgtgcat caggagtggc attcaatttt aatgcaacat ccacaatggc
     1141 tagattgagt tcatcggcag tttcaatagt atttatgatc tgtttgttta caaagcgtat
     1201 gaagtcatgt tttttcacat tacctgaacc ataagttggc aggttcacta ccgttccgga
     1261 gtgtggtgtg gcatctacag tgatcaacaa ggcatcaagt tttcctgtga tattgcaatc
     1321 tacttcacca atagcatatc cataaaaatc aggattttgc ttggcattgg tttccatcaa
     1381 taccagattg tcggaaatga catgtaactc tttgaaattc cagttatcga aatgactgta
     1441 ggtgacttca cccttcagcg atgcagtatt atttaattta tcggtgatgg tagtttccgg
     1501 tatggtgatc ttgttctttt ctacattgat atcaatctga ttaaatgcat aatgcgtgcc
     1561 catgtagtca actgtcatgc ctccattttt tacttggagt gacccttgca tttgaggtgt
     1621 gcttacaggg ccatcgatat ttattgtgcc atatatatct cctgacatat cggaaagtat
     1681 gccacgcaga aatggctcaa caataaataa tttggtgtga cggaaatcag cttttaattg
     1741 caagctgtct tcatcttcac cgcgacccat gatataatat ccatcggtaa caaatccatt
     1801 ttcaccacta agcgttgaat taaatttcaa tacattattg ggttgaatca atgatgtatt
     1861 taccttgaga ttaccgagca gtacatgatt taatgtaaga tcttttattt cagcaccacc
     1921 aagaaagctt ggatctttca acgcattgcc aatagtgtat gttgcatcaa gtgttccgct
     1981 gatatcttct cccggcaata caaagcgctc agccaattcg ccaataggaa tattgcggat
     2041 gccaagtttt agcacagtgc cgtaagtggc atctgcactg ctgctcaggc tgattacttt
     2101 attgccgcta ttaagggtga aattctgtgc atacagattt ttattatcgt atttcaagga
     2161 gttgttggga aggatgtccc atttttctgc ttcaaagaaa atttcagacg gaaggatatt
     2221 gaacataatg ctcgaatcta taaaggcaac ggcaccattt agattcacgc gatttgaatc
     2281 ggtatctttc ccggctttaa aattgtaatt taaggtattt tgagcgaagt tgccttcaga
     2341 cctcaggaca ggtaatacta cagctgaccc gatatgcgta gcattaatgc gtgtgaaata
     2401 ttccagagcg tcattagcag tgcgtgcttc gaacagcaag gtgtcaagcg taatattctg
     2461 ataattgaat tgatcgatgc gcagcctgtt gttgaatgag cgggtggcag tattgaaatc
     2521 gcctgacagg gttaaatttc gaacctcttg tatgtcgggg taaaatatgg ccattaatct
     2581 gtccgcattt tttatagaaa ctgtataatt cgccttttgt acttcgaggg tggaatcgat
     2641 gcgaccattt gtataataat ctactaggct tttaattgat tcatcgagtt tgcttggaag
     2701 gaaattcccc tctaattgta aatcgatgag agtagaatta gccttgatgc tttttttatc
     2761 gccaatttga gctgcagtaa aatttaatgt gtctaagcga taaatatatt tatcgcgaat
     2821 aagtaacaga tctccgaagt gcaaattgcc ttcaatattg tcgagtgaag ttccaccggc
     2881 atctgcagaa gctgttgcgc tgaaaactat tggttcggga tagaaattta attgcttcaa
     2941 gtttgcacgg gcaatatttg ctttaaaatc atagagtgga atgctgtcct gcatattgat
     3001 gataccattg aatgttagtt ttacattcgg atcatcaata gtgaaatttc cgctgaaata
     3061 atcattttca agattgccat caaccaccat attgctatag gtgtaatcat taaattccat
     3121 catactgatt tctgattgca tgtccatttc catttcatta tcacgagcaa tggatccgtc
     3181 gatggtggca tgcatgctga ttcttcccaa caaatcgggt tcgccggcaa taatgcctac
     3241 attaaatgca gaggtattca gatcgccgcg gaatgatgac tttccgagat cgggatcata
     3301 ttgaaaatgt acatcggtgt acatatttcc ctgatcgctg gtgaggtttc ccagcgtcac
     3361 aaggtcatag gcaaatccgg taacgcgacc ggtgtaattg atggtgccaa gcgcaagcaa
     3421 gttatccggg atagcacctg ctccgattat attttcaagt cgataggtat ttacatatag
     3481 tggttccaat tccatatcga taaatgtttc atcgatattc ggcaggcctt ttacatttaa
     3541 tgtgccacgc aatccggcag attcgtttaa catggcatcg aaatctcgac ccttaagatt
     3601 gtcaagtgct ccgtagatat gtccgctcag caataaatgt gcatcgtatt tatctaaaac
     3661 gggtgcgaaa tatgctaaat ctttattccc gacaattgtt ttatcgaaat tgccatccat
     3721 gcgcacatca gattcgaagc gatcaaaatc gttcagggta gtgaacgtca tcgcgaagct
     3781 tttctgaatt ttgctgttcg gtgttttaat taatagatct gtaagtgaaa tttcgtatgg
     3841 cgagaacaac acattcgcat gtaaagtgtc aacacggaat cctgattttt cggtaagtgc
     3901 aagatgatcg atgcgattca tgatagtgtc gccatgataa atgatatccg tcatttcaag
     3961 gtcgatcccg gtgatgtcca tgtcgctgaa attaattccg cgatttggcg ttgtgtcatt
     4021 cacatctaga aatgcaaatg cgcaattatg caatttcagt tgagaggctt ccatcaccca
     4081 gtcgcccatg gcaatatgca cggtatccca gctttccggt gtattgcttg gaatggtatc
     4141 tgttagtcct gtcaatgcaa accgcgtatc atcaatctgc aatttttcca aagcaataat
     4201 gtgatcttcg aggtcaaggg tgttgatgag cagttctaaa tcgccaacat gcatttgcat
     4261 atctgaagca gcaacttcat catgcaaaac aaaatgcacg tttttaagat ccagtctgtt
     4321 taatttcagt tgaacagatc ttcctccacc ggatgatgat gtgctcgtat ccttattgtc
     4381 attgctcgaa aatgcctctg aaatgaaatc gaaattataa attgagtcgg gcaaggttct
     4441 atcgagataa acacgtgtat cgtataatcc gatacttttg attttcacat aaggtacaag
     4501 tgggttgatg gtaccgatat tcacttccag tttcccggca tagagtaggg tgtcgtcttg
     4561 tttatcttct atatataatc cttccagaat aagatgatcg agcagatcaa tactcacttc
     4621 tttcacactc acttcggtat cgagtttttc actcagccat gaagtggttt tttccgctac
     4681 ccaattttga acggcaggga tgcgaagcaa caggaaaatg gtaagaaaca gcaacagtac
     4741 cgtggtcacg aagatgaccg tgatacgccg aaatatcgaa taaaccctcc aatatgtgaa
     4801 cagttgctgc actaaagcct tccgggtgtt atttttacgc tgttaagtta cgaatatcct
     4861 atgcctgtga caattttagg gatcgagtcg agttgcgatg aaactgctgc tgctgtgctt
     4921 atcaacggac agatcatgtc caatgtgata gcagggcaat cggttcatga gcaatacggt
     4981 ggtgttgtgc cggagctcgc ctcccgtgct catcaagcaa atatcgttcc tgttgtgact
     5041 gtggcgctta gtcgcgcggg catttcactc gccgaccttg atgccgtggc ttttacacag
     5101 ggacccggtt tgttgggtgc actcatggta ggcacggcat atgccaaagc actggcgatt
     5161 tcattggaca taccgttgat aacggttcac catatgcagg cacatatttt ggcccatttg
     5221 atagaagatc ccaagccggc atttcctttt ctttgtctga cggtaagtgg cgggcatacg
     5281 cagatcgtta gggtgaacag cgccatggat atggcaatcc ttggacaaac gatagacgat
     5341 gctgccggcg aggcttttga taaatgtgct aaaatgttgg gactgccgta tccgggtgga
     5401 ccactgatcg acaagtatgc cgctcaagga aatgcgcaag cttttgattt tggagagcct
     5461 aaaattcaag gattggattt ttcattcagc ggctttaaga catcggtttt gtatttttta
     5521 caagaacaga ctaaaaagaa tcccgacttt gtaaaaaata atctcaacga tctctgtgca
     5581 tccgtacaac ggggaattgt ggaaatttta ctcagaaagg tgaaacgtgc cgcaaaagaa
     5641 actggaattt cacgcattgc catatcagga ggtgtgagtg caaacaccgc tttacgcaaa
     5701 aaccttaccg atatgggagt ggcattgggc tgggaagtat tcattcctaa gttcgaatat
     5761 tgcaccgaca acgcagctat gattgccatt accggttatc agaaatttct tgcaggcgct
     5821 tttgctaggt acgacgttgt tcccatggca cgtattccgg gtatatacat ataattttga
     5881 aggcattttt atggcaagaa tccaattgac atttcctgaa aaaacacttt ttacaacggt
     5941 gcttgatgtg cgtattacgg atttaaatta cggcggtcat cttggaaacg atagtgtgct
     6001 ttcgctgttg catgaagcta gggtacgttt tttgcagtat ctaggtttcc gagagctgga
     6061 tgttgcagga actggtatta ttatgtctga tgctgccatc caattcaaag gtgaagcgtt
     6121 ctatggagag cagttgacca ttgcaatggc agttgccgat ttttcacgcg tgtcgttcga
     6181 tctttattat ctggtcacga ataatgataa aacaattgcg ctggcgaaaa caggcattgt
     6241 ttttttcgac tatacacaac gtaaaatggt tcctgtgccc gaagcatttt tacttgctgt
     6301 gaacagataa ttctccatca ttttagacaa tacacaaatt ttcgtgcagc atgatgttgt
     6361 tgccatggaa attttaatat tgatttgtat ttcgtttttc cgcttcgtta gcacacacct
     6421 ctccggcaaa tacacacaag aggcccgaaa cccacacgta tttcttaacc tctaattgtt
     6481 tgtgtgcgta tgaagttgtt ttactccgtg gcgttgtgcc tgcttggctt attttctttt
     6541 cctattacta tttctgctca gatgatggac gattgcgccg cccatcccga tgatgatgtg
     6601 atgcataccg agcactgtgc tgtatttgca ttggtgccgg aagatgctgc tacacatgtt
     6661 tcgattgcca atggatcatg gttctcacct gctacatgga atacaggaac ggttccggga
     6721 aacgaagcaa gagtgcttgt agatagcggt tttacagtta cttacgatgc cgttgccaca
     6781 gctgctatcg aatggatccg tgtaaatggt acattgaact tttcaactac agtaaatacc
     6841 caactgcgtg ttactgattt tattatagac ccgctggggc atttaaatat tggaacagtt
     6901 gcagcgccaa ttccgaatgg tgttacagcg aaaattcttt tcagcgacct tgggccaata
     6961 gattatatgg atgaccctta tcggtttgga aaaggattga taagtcatgg ttttttgaat
     7021 gtaaatggat attataaaaa gacattctgc gctgttacga aaaatctgaa tgccggcacc
     7081 aataacatta agcttgcaga aacgccaaca ggttggcaga ttggcgatca gttagttgtt
     7141 cccggcactt acggaactgc ttatggcgat ttcgatgcga acacgaaatt ccatgatgaa
     7201 gtggtaacaa taacttctat tgcaggtaaa acaatttatt ttacaaataa tgccacagga
     7261 acaagcatat tgcaatatga gcacaagctt cctgcagggt atggactgaa aatgtatgta
     7321 gccaacctaa caaggaacgt ggtgtttgaa tcagaaaatt acaatgtaat ccccgacgat
     7381 cagcgtggac atgtcatgtt gatgcataat atcggcgcat cggtgatgaa tgcgcaattc
     7441 aatggcttag gtaggacgaa taaagatatt ctggcaacag atcctgtagt agatgaattt
     7501 ggcaatttga tcagtggagg ggaaaatgtg cgcggcagat atgcattaca cgtgcacagg
     7561 gcaggaacaa ataatatcgc agctattcct gttcagatta aaggttgttc tgtagtggcg
     7621 gctaccagct ggggtgtcgt gaatcatgag agcaatgtca atgcagatga taatgtggtg
     7681 tttgattatt tcggtggtgc ttttgttaca gaagatggaa atgaactggg tacatttaat
     7741 cgaaacattg ccataaaagg tagaaaggca acaacggtaa ctgatctgac cacacggaca
     7801 cttaatatag actttgcatt tgaagggaat ggattctgga tacagagttc gaatgttggt
     7861 tacgaaaata atatcgttac cagctcagca ggtgatgcct ataaagtatt tagtgatgat
     7921 gcatctatgc ctgctgaata tcgtgtgaaa attcctaagg aaaatatatt aaatccgatt
     7981 attgcgggtg cagatgattc gatttatact gctgttgtgc cattgaggaa atttagtggg
     8041 aatattgctt acaactgcaa cagcgcaatg atgttttgga cacatatgtt gaataacgat
     8101 aatgtgggcg atttctcgac aattgcctat gatccatata cccatactat tatgagtgta
     8161 attgatggat gtaaattctg gaatatgctg ggagccggta tttctgtaaa atattccgga
     8221 caagtacatt ttaaaaatac tattttgctc ggcgatttaa ccaatcagta tcaaacaagc
     8281 gactggatag caggaaatcc ctatggcgga tatgctttta ttaccagtac tgttaccgga
     8341 caaattattt acgaaggtct tacggttaaa ggttggaaac gtggtgttgt tgcaggaaga
     8401 actgatgatt tgcaaagtgg tgataccaaa gagtacgact atagaacttc aaaaataata
     8461 ggtggcactt acgccgctaa tttatataat attgtgcctg aagaaggaac agatgtatac
     8521 ggtgcttctg agtattataa attccctgat tattttgaga tcagtggtag tccttcattt
     8581 acggcaataa caccaaatgt attgccgaca ccggattttg cctatttatc tgccggtgga
     8641 aatgccgtgc aatttaatgg cgctttatcg cttgatactg atccgggggt tataactccc
     8701 ggacagggca atggcattgc atcttatgtt tggaattttg gtgatggcag cagcggttat
     8761 ggaaaagatc ctgtgcatac ttataattta cccggcactt acagtgtaac attgactgtt
     8821 tatgatagcc aaggaaaaac tggtgtactt accagaaatg tatatgtttc tactacacaa
     8881 tattccaacg cggtagctaa ttccggcttt gagacaggtg gattaacaac ggcaagttat
     8941 atcaatagca cgcgtgcatt tgtcgataac ggatggattc acaaaagcaa ttggcagtta
     9001 ttggcaggga aagccaccat tttattgagc gataaatgga acagaccttt ggtgcaggta
     9061 attaaaaatg acaaggtatt gcgtggaaat gtgtctttta gttttcaggc aaaaaatttg
     9121 ggcatcggcg ccattggcaa tgacttatac gctgagattg ttggcgtcaa tggcgaattc
     9181 aaggatatga atttcacctc gttgggtgct gtgactaaat ggaatttgaa tgatgtggat
     9241 gtagttccta ctgtgcttta cagtgaaaac ctcggtcttg ccgaatacaa ctggcaaaca
     9301 tttaccagga ccgtgaattt tggaagcgga tatgattata tcattgtgaa attttattcg
     9361 caaggtgtaa aaaccggacc cggtgaatca caaggcattg ataatgtttg tttaccgtgt
     9421 gtttgtgctg tcccgggagg attgtttgcc gatgcattga cctcttctca tgcaacactg
     9481 atttgggata atgtgggtag cacgcaatat caggtgcaat ataaaacaac agcaggagct
     9541 acgtggacaa cagtaacggt agaaaatact tatctcgatc tttcttcact ttcggctaat
     9601 acatcataca catggaaggt gaaagcttgg tgcagtggtg catggacagc attttcggga
     9661 gataatattt ttattacacc ttcggctgga acaacttgta catcgcctag cgaattatct
     9721 acggagttga tcacacccaa taaagcaacg ttagtttgga atgccattcc gggagcattg
     9781 caatatcagg tttcatataa atcattgctt ggattgacat ggacagatgt tacaacaaca
     9841 aatactactt tatcattaac cggtctttta ccattaactg catataattg gaaggtaaaa
     9901 acgcaatgcg ctgccggatg gaaagattat actacattgt tgacattcac tacactaccg
     9961 ttgcgtgaag gcgaagaaat tggggaaaat gacctcaata ttcaggcatg gccaaatccg
    10021 gtatcttatg aattgcaatt gacatttagc gaggaaatca gtggtacagg cagtttacga
    10081 ctaattgaca tttccggaca aattgttaaa gccattgata ttcagataga tggcacaaac
    10141 attgcgatac cggtaaatgt atctgattta ccgaccggaa tttatgtgtt gcaagttgcc
    10201 ttgataaacg ggcaaatgtt taacacgaag atcattaaag aataaaaggt ggttctttta
    10261 tgaatagcgg cccggtatca gtgcgatatc gggcttttta ttttctgtta tttatcattc
    10321 atgattaaaa ccaacaacat gcatactcga attttcacat ttatgatcct ttgtgcgatg
    10381 gccactgctg tcaaggcaca aaacaatgaa ctccatgcag taggtatccg tttcggtggt
    10441 ggcgatggtg taggtgccga aatttctttt cagcaataca tcggaggtcc caataggttg
    10501 gaatttgacc ttggcgcttt tgaagatggg tataacgatg gatttaaatt aactgttctc
    10561 tatcaatggg tgcattccat cgacaattca ttttattggt atgccggtct tggaggcggg
    10621 ctcggcgctc gggatcatga caatggccat ccgtttgaag gcgatgacga cgatggtgtt
    10681 tttgttgatt ttgatttcga cctcggaatg gaatacaatt ttgatttccc attgcaatta
    10741 agcctcgata ttcgccctga gatcggattg atcaacgatg attttgatat gggctgggca
    10801 ctcggtgtgc gataccggtt ctaatggtgg acagcaaggt ggaatggtta atgttcatca
    10861 ttgctccgag gtatttatac aattataaac gcgatagtat gcacagtgcg tgagttacta
    10921 tcgcgttttc ttttacttaa tgaaattagt atttgatcat tgctgctttg ttatgattgt
    10981 ttgctgttct cccacaacaa ccagatacat tcctgacggt tggttagcga gtgaaatgct
    11041 ggtgggtaat tgtacttgat caagctgata taaaacacgt ccgtcaagtc cataaacagc
    11101 cagtttgcat ggtgatgtga aatctccatt gatatatatc atatcgctgg ttggattcgg
    11161 aaaaatgtgt atatcgaatg cagtatttgt tgatatatca gtttgtgtac tgcaatccag
    11221 tgcgcttgca atatatgcgc tgcgattggt gacgaaggat tttaatccat acgtggtgcc
    11281 acccattccg ccagcaatat ctgattcaat attcgtttca aactgatcat tagtatacat
    11341 cttattcgga tcggcataaa catgcggttg aatgagtgta tatagctcgt cgatcaaatc
    11401 gctgatataa ctattgttga aataggcatt gaacaactcg caataatacg tttcgtactg
    11461 tgttttcgta tcttctattg taagcacgcg ctcgaataaa ggtcggttca gattatagta
    11521 atatatatct aattcctgca gatcaccggt tccggggccg ccggtgtaaa gaccgaatga
    11581 ctcattgcag tcccagttga tccattccca aatgcctgtg ctgtcgtaat catagatata
    11641 ataattgcgt gcagaattga gatagctatc cagattgctg aacatattat acatcaccat
    11701 gcatttcatc aattgttcta catcgaaatg atgtgatagc tgtccggcaa aatcagcatc
    11761 ggaagaatta ttgatgaaat cagtgaggtt gatcagatct gtccagtcgt tttcatcggt
    11821 attgttctct aacgtatatc tgtcgtaata atcatcctgc acagtaccat aatatttcaa
    11881 atctccttca acaccgccac cgggtccgcc accaaaggca tcacctgctt taaaaagatt
    11941 ttcattgttc tcgtcaaaat gtgttttcaa aaattcatca tctacttgtt caacgacgga
    12001 ataaaatccc cataattcat cgttcatata tacatcagca taattagctc gcggtgcatg
    12061 aatgctgtcg gccttacaaa tgtcataata gatcttctca cgcataaagc ttgggtcttt
    12121 aaagctgttg ttgaaattta atttcctcaa gccgtcatat tcttgccctg ctacataacg
    12181 attgaaatct attttgaatg attttttatt tcccgggtga ccataacttg aatttccctt
    12241 caatcgcaca ccaacactgt cgaaggtata ggtgcctgta ttatcggtaa cagtgagatc
    12301 agccatcata tatgtttctg ttgcatagtt ggcgaccaga ctatcccaga atccgggctg
    12361 agagaattgc agatctatgg tcagtacttg attggtgtta aagatattgt cgcctggatt
    12421 ttgtgccata gctacagagg caaaacctaa tgccagcagg gtggttctaa tgtgcatgct
    12481 gtgaaatttg tggtatgacg gtctttggga aaaaaggttt aattgtaaaa ccgggttttt
    12541 taggaagtct gatttccggc tattgacctg gccgccagcc accctgttgt ccaagctgcc
    12601 tggaagttga atcctccggt aacagcatct atattcaaca tttcgccacc gaaatatagg
    12661 ttgggatgta gtcggctttc catgttgcgc atattgactt cgttgagcgc aatacctcca
    12721 caggtaacaa attcttcctt gaaagtgctt ttgccgttaa tgctgaaggt ggatgaggtt
    12781 aatatttcag caaagctttc tatcattatg tttgacaaat ctgcccaatt gtgattttcg
    12841 gttatgttgc caaatgcaca aagctgcttc cataatctgg caggaatagt tgggaaatga
    12901 tcattcacta ctttcttttt cggatgatcg tttcgcattc cacgcaaata atcgatgcat
    12961 gatgcgctat catatgttgg gcaaaaattg atttgtatgc ttgcgcgata ttgttttttc
    13021 gccaaaacat cagctcccca tgcagaaagt tttaatattg ccggaccact caatccccaa
    13081 tgtgtaatca gtaatggtcc ttcggtgata aggtctgtat tcataagcga tgcctgcaca
    13141 tgaggaatgc taactcccat taatccatcg atgcgtttat cgcgaatatg aaaagtgaat
    13201 aaacttggaa caggtggaat aatggtatga ccaagttgct ttaataaact ccacgcttgc
    13261 tgactgctgc cggttgccat cataaccgca atggcatgaa atgtttgatt tttgcaatca
    13321 attttccatg cattatcaac tgaggtaatg gattcaactt gcacaccaaa atgaatatgc
    13381 acgtgatatt tagttgcttc acgaagaaag caatcgataa ttgtttggga agaattggtc
    13441 acaggaaaca tgcgaccatc aacttcagta tgcaattcta cacctcgatc ttcaaaccat
    13501 tgaatcgtat cagcaggttg aaaatgatg
//
LOCUS       DAOQHY010000088        13529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2203_length_13529_cov_5.546831, whole genome shotgun sequence.
ACCESSION   DAOQHY010000088 DAOQHY010000000
VERSION     DAOQHY010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13529
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2203_length_13529_cov_5.546831"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..327
                     /gene="purS"
                     /locus_tag="PLL28_08510"
     CDS             76..327
                     /gene="purS"
                     /locus_tag="PLL28_08510"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HNF69402.1"
                     /translation="MAYKAEINVMPLKELLDPKGKAVAGSMNNLSLTAIKDVRIGKRI
                     QLHIEASSASEAKRIAEEACKKLLANPIMESYEISISEG"
     gene            345..1019
                     /gene="rsmI"
                     /locus_tag="PLL28_08515"
     CDS             345..1019
                     /gene="rsmI"
                     /locus_tag="PLL28_08515"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNF69403.1"
                     /translation="MKGKLYIVPTPIGNLEDITFRAIRILKEADLILAEDTRTSSVLL
                     RHYGITTKAISHHKFNEHAQVQQLAQEIAEGKQIALISDAGTPTISDPGYLIVRTCLD
                     HALPVETLPGATAFVPALVNAGLPMHEFVFIGFLPQKKGRETKLRSLAAEQRTMILYE
                     APQRVVKLLEQLLQHLGDRRASVSRELSKKFEETQRGLLSELIKHFSEKEPKGEFVIV
                     VEGLQD"
     gene            complement(1030..2430)
                     /locus_tag="PLL28_08520"
     CDS             complement(1030..2430)
                     /locus_tag="PLL28_08520"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HNF69404.1"
                     /translation="MRRGKDILITILCISCMCRYMHAQEFNGLRTDNYAGSYGMLLNP
                     AMPITGKIPWDVNFVAFGVNEDNNYIKISDPVFKYVTTSDSILDVEYYNPTQIRGHGN
                     VLLQLPSAFIKVDDYAFGMFTNARSAGYFLSDKEPGGVIGLNDLPLYTDLNMPAFNAG
                     ILVWSEIGLNAEMTLESNSLFSLHIGANIKYLMGLEALAFENNELFTFVKDTVNFTMT
                     NFNTDFDYTRNLGSNLLYDPSNYALNGSGFGIDLGAYYVIHKKSKFKYSKKLDYTWKF
                     GAALLDFGAIKFQSNSGSYHLEEDQLFSVATLVLDSIDDIDEFNRTGSKTLYDNVDAS
                     KDASKFAMYLPASFLLTVDKPLRNDFYVQAMIMRRIPRFSTNLIARSNVFGLTPRYER
                     RKFGFFLPMQLYEDREFRIGTAVRWHFVTVGSDNLRTWFTPKEYSSIDFYVGVKLTPY
                     WLVTQSKKKYLECLKN"
     gene            complement(2597..3997)
                     /locus_tag="PLL28_08525"
     CDS             complement(2597..3997)
                     /locus_tag="PLL28_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNF69405.1"
                     /translation="MFTKLNAAHIARLTEIVGAAYAFLGNDQLLEYGSDETEDLSFPP
                     EIVLKPRTPEQISEILKFANDHMIPVTPMGARTGLSGGALPVFGGIALSMERFNEILH
                     IDERNLQVTTEPGVITEVLQNAVRDKGLFYPPDPASRGSCFIGGNIAENSGGPKAVKY
                     GVVKDYVLNLEVVLPTGEIIWTGANVLKNATGYNLTQLLVGSEGTLGVITKIVLRLIP
                     HPQRDMLLLIPFPSAVKACAAVAEIFKAGITPSAMEFMEKDAILYVLKHNPDLHLDLP
                     EGIEAHLLVEVDGNNPDLLMSEIEAISGIVEQFECGEVLFAESEREKQDLWRIRRKVG
                     EAVKQHSVYKEEDTVVPRAELPQLLAGVKEIGAKYGFQSVCYGHAGDGNLHVNILKGN
                     MTDASWDNTLSIAITEIFQLVKQLGGTISGEHGIGLVQKPYMNIVFDEVHLNLFRKLR
                     KVFDPNGILNPGKVVD"
     gene            4162..6213
                     /locus_tag="PLL28_08530"
     CDS             4162..6213
                     /locus_tag="PLL28_08530"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HNF69406.1"
                     /translation="MKRFFYSLPVVALLFVTATAFGQAKKYPLFEHFTQASCGPCAAQ
                     NPYFQAVYYANESNLHHVAYHTSWPGIDPMYDFNSAESDAMVDYYGVTGVPDMIQNGS
                     DIGSPAGVTQDIVNNLLSETSPIRILVTESTTGTTRNVHIEVVTVGTVPAGTYKLKAA
                     VVEHIVDYVTAPGTNGETEFPNVFRESITGTSGVAITPAAIGSSVSFDYSYELDPIYV
                     DENIYALAWVQNSSTKEVLNSGSASDPDVEVINTSAATFVQGTVGGATPFTGNILNLN
                     DAEGMNMHFEFAADQPGDWSASYTYDGTTYTGATDAYIAGAGDKDVTFNINVGASAAI
                     GEYYLTVTFPDNPEISAQVLSYYVISGVTDLVVNNEVSFGDGSAYGTYDWESLYVDGL
                     TAAGVSTVAATSHFTMKKGFNSGALDGIDNIYYNVGWTFPGLVDDAEKIAQLMDFLDN
                     GGNLFISGQDIGWEVNEYAAYYPEAVDFYENYLMASFVNDAASGATVFSPVLTDEWYG
                     AVAESDISKPYGTTYYYPEQIEPNGVDATSVFTYNAGTKVGGVRAETSDFKTVYFGIG
                     IEMIADDAVRNAVMAATYAYFRGDLEGIDYDALISGLMGGAQPNPAVNSTVIPMTNIN
                     SDYQLTVTDLTGKVILTDIVKAGTSSYTLNTADLSAGMYLYYMSSGNSRTQTEKLNVV
                     K"
     gene            6342..7076
                     /locus_tag="PLL28_08535"
     CDS             6342..7076
                     /locus_tag="PLL28_08535"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69407.1"
                     /translation="MKLRLLLTVFSLGAFAAFGQITLTPSTAELDGDPSVIQEIHIDF
                     HNASGAKQTMTWLRTMNDIPDEWTTSVCDFNLCWAPTADEPGYYFDAPIDTTGTVYVK
                     FDARNYHDGAFNPIPGCGTVEVHFFSVLDSADNNAVALFHARLGVSEEDCGVDVVSSV
                     LDNSFAIYPNPAVNNINVIASFSANIAKVDIVNIVGRTVRTYDWNTGSGKMSFDISDL
                     PQGVYFARLIDEAGKVVYAEKVSVLK"
     gene            7234..9891
                     /locus_tag="PLL28_08540"
     CDS             7234..9891
                     /locus_tag="PLL28_08540"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HNF69408.1"
                     /translation="MKRIILLLLTFWVLGTQAQNIAINTDGSTADNSAMLEIKSSTKG
                     FLVPRMTAAQKTAIPTPATGLLIYQTDGSTGFWYYNGAAWTQISGSAGWSLTGNAGTV
                     AGTNFIGTTDDVALQFKIDNTVAGGINKNGNTSLGYKSLQNTTTGTSNIAIGKMALNA
                     NTTASGIVAIGDSSLSKNGTGAAFLWEGANNTSVGNRSSKNNTTGYDNTAIGSYSLQN
                     NTTGANNTALGSSALYYTTTGNNNLAAGSYSLLFNTTGAYNVATGQSGMVSNTTGSYN
                     TASGFASLFNNTTANYNVANGTYSGYYNTTGNRNTFVGYGAGYFNTTGYLNTCIGSQA
                     GYSTTTAFSNVFVGEQAGYSNTTGYSGVYIGQDAGYFNTTGVENTFIGTDAGEANTTA
                     SYNTFVGTNSGLLNTTGYQNTFVGQDAGRANTTGMWNTFLGEDAGYANTTGNFNTLVG
                     VNAGQANTTGSKNIFIGVGAGHDNTTASNNIVIGTDAALSGTTGSNNVFIGTESAYTN
                     TTGFDNTYVGNYGGRSNTTGYYNVGVGKNVLYANSTGIWNTASGAFSMMDNTTGSYNT
                     AFGINALRYQTTANYNVALGATSMQQTTTGNQNLSAGYAALLTNTTGSSNVALGYQSL
                     YFNTTASWNTATGYMTMYNNTTGSVNVASGYSALYSNTTGTNNVALGSSALIGNTTGA
                     RNVGVGSGAGDLNDNNTNCTFLGYDADQAVTTDFDNSTALGNGSRITASNQVRVGNAS
                     VTSIGGYVGWTNISDGRVKKDVTENVPGLAFIMALKPVTYHLDIDAINDHLHVADSVQ
                     NLQSIAAKNKVLYSGFIAQDVAAAAKQIGYDFSGVDAPKSEDDLYGLRYADFVVPMVE
                     AMQEQQKLIEKQQEQIDALTKLVQSMQNH"
     gene            9893..11143
                     /locus_tag="PLL28_08545"
     CDS             9893..11143
                     /locus_tag="PLL28_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69409.1"
                     /translation="MRITLLLFGLLSCIAASAQSRLTMNDNPYIVMNGSVQMVIDNNN
                     ANAITTTGSGGNIISESETNIISWHINTATGSYIVPFTTSDGVKIPLTVQITSAGAGA
                     GVIDFSTYGGLTWNNDTYKPGEVTNMTNMGVTNNSTEIIDRFWIIDAHGYTTKPSGNI
                     QFNYDDAEHLAAGNTINEPDLKAERYEPGTNNWEVFPVGGIINTGSNYVTGVPFNSGD
                     FMRTWTLIDQTTHLLPIVLTNFSATCVAGGTNLQWTTASEINSDHFIIAGSTDGIYFE
                     DLADIQAAGNSNTPTSYSYTADDNMFRYYKLSLLNTDATIEELGILSTSCNQQITPEF
                     FAYSPASFQITAQTQQLTPGNYVISIFTLQGQLITGSNLQLGTEVATMTYYDQSLSDG
                     IYIVALRSTDNPKITMTSKVALFH"
     gene            complement(11152..12936)
                     /locus_tag="PLL28_08550"
     CDS             complement(11152..12936)
                     /locus_tag="PLL28_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNF69410.1"
                     /translation="MSSNNLILKGGSFLLHQSTPSDIFIPEELNEEQLMIKQMTTDFV
                     QKEVLPNLDRIDKHEEGLMAALLTKAGEIGILGLPIPEAYGGFAKDFNTISAVTEVTG
                     TAHAFSVAYGAQSGIGTLPILYFGTEEQKKKYLPGLAAGTTHASYCLTEPGSGSDALA
                     AKTKAVLSEDGKHYILNGQKMWITNAGFADIFTVFAKIDGDKFTAFIVERGAPGFTIG
                     VEEEKMGIKGSSTCQIFFENTPVPVENVLGEIGKGHLIAFNILNIGRYKLCVGVLGGA
                     KALIDTSVKYANERMQFNVPIASFGAIKYKLSEQAIRTFAALSATYRTSDYIDKKEKE
                     LLSKGIGHEKALLGAAEEYAIECAMLKVIGSEVLDFVVDECVQIHGGNGYSEEYPAAR
                     AYRDARINRIFEGTNEINRLLTVDMLLKRVMKGQLDMLGPAMAVQKELMGIPELGETG
                     EGLFYEEEKAIENMKKAFFMVAGAAVQKLMMALKDEQEILMCAADMMNEIFTAESMLL
                     RIKKLTLQNGEDATSIHRDMLAVYFTDSIDRLNKQGRTAIASFATGDELRMMLLGMKR
                     FTKYRTVNTTAARRRIADKMIAENGYCF"
     gene            complement(13014..13376)
                     /locus_tag="PLL28_08555"
     CDS             complement(13014..13376)
                     /locus_tag="PLL28_08555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNF69411.1"
                     /translation="MHNYKSLIVWQNAMELVKEVYLTTKTFPKEEIYGLVQQIRRSAI
                     SIPSNITEGCGRKSDKDFDNFLSIAHASSFELETQLMIAESLSLIKNEESQLLLSKTS
                     LVQKKLFKLKESLKLKNN"
BASE COUNT         4016 a         2796 c         2783 g         3934 t
ORIGIN      
        1 atattctttt tgcagtgatc ccgatgatca tatttgcata cgttatactt tccatcacta
       61 aaaatattac agaaaatggc atataaagca gagatcaacg taatgccttt gaaagaatta
      121 cttgatccaa aaggcaaagc tgttgcgggc agcatgaata atttatcatt aacggcaatt
      181 aaagatgtgc gcatcggcaa acgtattcaa ttgcatatag aggcatcttc tgcttcagaa
      241 gcgaaacgca ttgctgaaga agcctgcaaa aagttgttag cgaatcctat catggagagc
      301 tatgaaattt cgatatctga agggtgatat gatatatatc tgcaatgaaa ggtaagctat
      361 atattgtgcc gactcccatt gggaatttgg aagacattac ctttcgcgca attcgtatac
      421 ttaaagaagc ggatctgata ttagcagagg acacgcgcac atcatctgta ttacttcgtc
      481 attacggaat aacgacaaaa gccatttcgc atcacaagtt caatgagcat gcgcaggtgc
      541 aacaattggc gcaagagatc gccgaaggaa agcaaattgc gctaatttct gatgcgggca
      601 caccgacaat ttcggatccg ggatatttaa ttgtacgcac ctgcttagat catgcattac
      661 cggttgaaac tttacccgga gcgaccgcgt ttgttccggc tttggtgaat gcaggattac
      721 cgatgcatga atttgtattt atcggattcc tgccacagaa aaaaggtcgg gaaacaaaat
      781 tgcgttcctt agcagcagag cagcgcacca tgattttata tgaggcgccg cagcgtgttg
      841 taaaattgct ggaacaacta ttacaacatc ttggcgacag gcgtgcttct gtaagcagag
      901 aactttcaaa aaaattcgaa gaaacacaac gcggtttgct cagcgaatta ataaaacatt
      961 tttcagagaa agagcctaaa ggtgaatttg taattgttgt ggaaggatta caagactaat
     1021 caggcgattt caattcttca agcactcgag atatttcttt ttactttgcg taaccagcca
     1081 atacggagtg agttttacgc caacataaaa atcgatggag ctgtattctt ttggtgtaaa
     1141 ccaagtgcgc agattatcag atccaacagt gacaaagtgc catcgcactg cggttcctat
     1201 tctaaattcg cgatcttcat ataattgcat gggcaggaag aatccgaatt ttcgtctttc
     1261 atatcttggt gtcaatccga atacattcga tctggcaatc aggtttgtgc tgaatctggg
     1321 aatacggcgc atgatcatgg cctgcacata aaaatcgttg cgcagtggtt tatcaactgt
     1381 aagtaaaaat gatgccggca gatacatagc aaatttgctt gcatctttag aagcatccac
     1441 attatcatat aatgttttcg aaccggtgcg attaaactca tctatgtcat caatagaatc
     1501 gagcaccagt gttgcaactg agaatagttg atcttcttcg aggtgataac tacctgaatt
     1561 tgattgaaat ttaatcgccc cgaaatcgag caaagcagct ccgaatttcc aggtataatc
     1621 aagtttttta gagtatttaa atttcgattt tttatgaatg acataatatg ctccgaggtc
     1681 aattccgaat ccgctgccat ttaatgcata atttgaagga tcgtataaaa gattggagcc
     1741 taaatttctg gtataatcaa aatcagtatt aaaatttgtc atggtgaaat taacggtatc
     1801 tttcacaaag gtgaagagtt cgttattttc aaatgccagc gcttcaagcc ccatgaggta
     1861 tttaatattg gcgcctatat gcaaagagaa tagcgaattc gattccaaag tcatttcagc
     1921 gttcagacca atctcactcc agaccaaaat tcctgcatta aatgcgggca tattcaggtc
     1981 agtatacagt gggaggtcat ttaagccaat aaccccgccg ggttctttat cgctcagaaa
     2041 atagcctgca cttcgagcgt tggtaaacat cccaaaggca taatcatcca cctttataaa
     2101 tgcagaaggc aattgcaata aaacatttcc atggccgcga atttgggtcg gattataata
     2161 ttcaacatct aaaatagaat cagaggtagt gacatattta aaaaccgggt cactgatttt
     2221 gatataatta ttgtcttcgt tgacaccaaa tgcaacaaaa tttacatccc atggaatttt
     2281 gccggtaatc ggcatcgcgg ggtttaaaag cataccgtaa gacccggcat aattatcagt
     2341 gcgcagacca ttaaattcct gtgcgtgcat atacctacac atgcacgata tgcataaaat
     2401 agtaatcagg atgtcttttc cccttctcat gcagtaaaca gattagtcgc tgtgtgtgcc
     2461 ctcactagct ctgcttgtgt gtgctgcatg caatccgctt ttcacatgaa acggattaac
     2521 aggtataaag ttgtgtaaaa attatgacaa aaaacagact ttttcgcaga aatttcaata
     2581 attatttgac gacttgtcaa tcaacaacct taccagggtt taaaatgcca ttaggatcga
     2641 acacctttct gagcttgcgg aacaggttga ggtgaacttc atcgaataca atgttcatat
     2701 aaggtttttg aaccaggccg attccatgtt cgccgcttat agtgccgcca agttgcttta
     2761 ctaattgaaa tatttctgtg attgcaatac tcaaagtgtt gtcccacgag gcgtccgtca
     2821 tattgccctt taaaatattc acgtgaagat ttccatcgcc ggcatgacca tagcaaacag
     2881 attggaaccc atattttgca ccaatttcct tgaccccggc cagcaattga ggtagttcag
     2941 cgcgaggcac cacggtatcc tcttctttat aaacagaatg ttgcttcacg gcctcaccca
     3001 cctttcttct gatacgccag aggtcctgct tttcgcgttc agattcggca aacagcactt
     3061 caccacactc aaattgctct acgatgccgc ttattgcttc gatttcgctc attaacaggt
     3121 ccgggttatt gccatccacc tcaaccagca aatgtgcctc aatgccttca ggaaggtcga
     3181 gatgcaggtc gggattgtgt ttcagcacat aaagaatggc atccttctcc atgaattcca
     3241 ttgcgctagg ggtaattcca gccttaaaaa tttctgcaac agcggcacaa gcctttactg
     3301 cgctggggaa tgggatcagc agtaacatat cgcgctgagg gtgaggtatc agccggagaa
     3361 cgattttggt gatcacccca agtgttcctt cgctgcctac aagcaattgg gtaagattat
     3421 atcctgtagc atttttcagc acattagcac cagtccaaat gatctctccg gttggcaata
     3481 cgacctcgag gttgagcaca taatccttca ccacgccgta tttcacggct ttggggcctc
     3541 ctgaattttc ggcaatgttt ccacctatga agcagcttcc tctgcttgca gggtcagggg
     3601 gatagaatag gcctttatcc cgcacagcgt tttgcaaaac ttctgttatt actccaggct
     3661 ccgtagttac ctgcaaattc ctttcatcaa tatggaggat ttcattgaaa cgctccatac
     3721 tcagggctat acctccaaaa accggcaagg caccaccgct tagccctgtg cgtgcgccca
     3781 tgggggtgac aggtatcata tggtcattgg caaacttcag gatttcagaa atctgctccg
     3841 gagtgcgagg ttttaagaca atctccggag ggaatgagag gtcctctgtt tcatcacttc
     3901 catattccaa caactgatca tttcccaaga aggcataagc agcaccaacg atttcggtca
     3961 gtcgggcgat atgggctgca ttcagcttgg tgaacatgag atcaaaggta taaacctcat
     4021 tagaattgtc atggagaatt gccaaccacc ctgcgttagc atttactgat gcttgacatc
     4081 tgtcaaatta ggttcattcc tcccaaatgt tttgctgatt taggaggaag gcgtatcttt
     4141 aatattcaaa ctacatcaga aatgaagaga tttttttact ccttaccggt agtagcgcta
     4201 ttatttgtga cagctaccgc ttttgggcag gcgaagaagt acccattatt tgagcacttc
     4261 acccaagcat cgtgtggacc ttgtgctgca cagaaccctt attttcaggc agtatattat
     4321 gccaatgaat ctaacttaca ccatgttgcc tatcatacca gctggccggg catagaccca
     4381 atgtatgatt tcaactcagc tgaaagcgat gccatggttg attattatgg cgttaccggc
     4441 gttcctgata tgattcaaaa cggatctgat atcggaagtc ctgcaggtgt aacacaagat
     4501 attgtgaaca accttttatc tgaaacaagt ccgattcgca tattggtaac tgaatcgaca
     4561 acaggcacca cccgcaatgt gcatatagag gtggtaacag ttggtactgt tcctgccggc
     4621 acatacaaat tgaaagctgc cgttgttgag catattgttg attatgttac tgctcccgga
     4681 acaaatggtg aaactgaatt tccaaatgtt ttccgtgagt caattacagg aacaagtggt
     4741 gttgcaataa cacctgcagc tataggatca agtgtttcat ttgattacag ctacgaacta
     4801 gatccaattt atgtagacga aaatatttat gcattagcat gggttcaaaa ttcatcgaca
     4861 aaagaggttt taaattcagg ttctgcaagt gatcctgatg ttgaagtgat taatacttct
     4921 gctgcaacat ttgttcaagg aactgttggt ggtgcgacac catttacagg aaatatcctg
     4981 aatctgaatg atgctgaagg aatgaatatg cattttgaat ttgctgccga tcaacccggc
     5041 gattggtctg catcatatac ctatgatgga acaacctata ccggtgctac tgacgcttac
     5101 attgccggag caggtgataa agatgtaacg tttaatatca acgtaggagc atcggctgct
     5161 atcggagagt attatttgac agttaccttc cctgacaatc cggaaatcag tgcacaggta
     5221 ttgagttatt atgtgatcag tggtgttact gatctagtag taaacaatga agtaagtttc
     5281 ggcgatggct ctgcttacgg cacttacgac tgggaatcgt tgtatgtaga tggtcttacc
     5341 gccgcaggtg tttccacagt tgctgcaact agtcacttca caatgaaaaa aggattcaac
     5401 agcggtgctt tggatggtat agataacatt tattacaatg ttggatggac attccccgga
     5461 cttgttgatg atgccgaaaa aattgcacaa ttgatggatt tcctcgataa tggaggtaat
     5521 ttatttattt ccggtcagga tataggatgg gaagtaaatg aatatgctgc atactatcct
     5581 gaggccgtag atttttatga aaattacctg atggcaagtt ttgtaaatga tgccgcttca
     5641 ggtgctactg tattcagtcc ggtattaact gatgaatggt atggtgcggt tgctgaaagt
     5701 gatatcagca agccttatgg aacaacctat tattatcctg agcaaatcga accaaatggt
     5761 gtagacgcaa caagtgtatt tacttacaat gcaggaacaa aagttggtgg tgtgcgtgcg
     5821 gaaacttcag atttcaaaac tgtatacttc ggtattggta ttgaaatgat agctgatgat
     5881 gcagttcgca atgcagtaat ggctgcaact tatgcttatt tccgtggcga tttggaaggc
     5941 attgattatg atgcgttgat tagtggttta atgggtggcg cgcagccaaa tcctgcagta
     6001 aacagcacag ttattccaat gaccaatatt aacagcgatt atcaacttac agtaacagat
     6061 ctgaccggca aagtgatttt aaccgatatc gttaaagcag gcaccagcag ctatacattg
     6121 aatactgctg acctcagcgc aggtatgtat ctttactaca tgagttccgg taacagccgt
     6181 actcaaactg aaaaattgaa cgttgtaaag taatcttaat acgtcataaa agagtcaggt
     6241 acatcaacct ggctcttttt tttgcctatt ctgcactatt tttgtagtat aaaccaacgg
     6301 agcaccacaa taaaccgtta acaatattat caactcttat catgaaactt agattactcc
     6361 ttacagtatt ttcgctgggc gcatttgctg cttttggcca aattacgctc actccttcta
     6421 ctgcagaact ggatggtgac ccttcagtaa tacaggagat tcacatcgac tttcacaatg
     6481 caagtggtgc taaacaaacg atgacctggc ttcgtactat gaatgatatt cctgacgaat
     6541 ggaccacttc tgtttgtgat tttaacttgt gctgggcgcc gacagctgat gagcccggtt
     6601 attatttcga tgcgccaatt gatacaaccg gaacggtata tgtgaagttt gatgccagaa
     6661 attatcatga tggcgccttt aatccgattc ccggatgcgg aaccgttgaa gttcattttt
     6721 tcagcgtttt agacagtgct gacaataatg cggttgccct tttccatgcg cgtttgggtg
     6781 tgagcgaaga ggactgtgga gttgatgtag tttcatcagt acttgacaac agttttgcca
     6841 tttatcccaa tcctgcggtt aacaatatca atgtgatagc ttccttcagt gcaaatatcg
     6901 ctaaggttga tattgtcaat atcgttggtc ggacagtcag aacatacgat tggaataccg
     6961 gaagcggaaa gatgagtttc gatatcagtg accttcctca gggcgtgtat tttgctcgtt
     7021 taatcgatga agccggcaaa gttgtgtatg ctgaaaaggt atcagttctt aaataaacag
     7081 acttagcaaa ctctttatag aaggctccat ttggggcctt tttttatgcc tatagggttc
     7141 atttgggtgg aatagtctgc tacaatttca aaaatttcta ctggtttcaa ggctttagaa
     7201 taagtcactt tgcggtattc taaatgctgt tgtatgaaac gtatcatctt actcttattg
     7261 accttttggg tattgggaac ccaggcccag aacatagcta tcaataccga tggctctaca
     7321 gccgacaatt cagccatgct ggaaatcaaa tccagtacta aaggattttt ggttccaaga
     7381 atgaccgctg ctcaaaaaac ggctattcct accccggcta ccggcctatt gatctatcag
     7441 accgatggct caaccggttt ttggtattac aatggagcgg catggacaca gatttcagga
     7501 tctgccggat ggagtttaac cggaaatgcg ggaactgttg ccggaaccaa ttttatcgga
     7561 accacagatg atgttgcctt gcaatttaaa atagacaata ctgtggcagg aggaatcaat
     7621 aagaatggaa acacgtctct aggatacaaa tcactgcaaa acacaaccac aggcaccagt
     7681 aacattgcta taggcaagat ggcattaaat gcaaatacta ccgcttcagg aattgtagca
     7741 attggcgact cttcactatc gaaaaacggt accggtgcag catttttatg ggagggagct
     7801 aacaatacca gtgtaggtaa caggtcttcg aaaaacaaca caaccggtta tgataatacg
     7861 gccattggaa gttattcgct tcagaataat acaactggag caaacaacac tgctttaggc
     7921 tcaagcgcct tatactacac aacaacaggt aataataatc ttgctgcagg ttcatatagc
     7981 ttgcttttta atactaccgg cgcatataat gttgcaacag gtcaaagcgg tatggtttcg
     8041 aataccactg gttcatataa taccgcctcc ggatttgcat cattatttaa taatacaaca
     8101 gcgaattata atgtagctaa cggcacatat tcaggatatt acaataccac cggcaacaga
     8161 aacacttttg ttggttatgg cgcaggttat tttaatacta caggatatct aaatacttgt
     8221 attggatcac aagcaggcta ttcaacaaca actgcatttt caaatgtatt tgtaggtgag
     8281 caagccggtt attcaaatac tacaggttat agcggggttt atataggaca ggatgcagga
     8341 tattttaata ctacaggcgt agaaaataca tttatcggaa ccgatgccgg cgaagcaaat
     8401 acaacagcaa gttacaacac cttcgtcggc acgaattccg gtttgctcaa cacaacaggt
     8461 tatcagaata catttgttgg tcaggatgca ggaagggcga atacaacagg tatgtggaat
     8521 acatttcttg gtgaagatgc cggatatgca aacactacag gaaatttcaa cacactggtt
     8581 ggtgtaaatg ccggacaagc aaatactacc ggttcgaaaa acatttttat cggcgttgga
     8641 gcaggacacg acaacactac tgcaagcaat aatattgtta taggaactga tgctgcatta
     8701 tcaggcacaa ccggaagcaa caatgtattt attggaactg agtcggcgta tacgaataca
     8761 accggttttg ataataccta tgtaggaaat tatggtggta gaagcaatac aaccggttat
     8821 tataatgtag gagttggaaa aaatgtgctg tatgccaact ctaccggaat ttggaatacg
     8881 gcatcaggtg cattttcaat gatggataat actacgggaa gttataatac tgcttttggt
     8941 attaacgcac tcagatatca aacaacggca aattataatg ttgcgttagg tgctacatca
     9001 atgcaacaaa caacaacagg aaatcaaaac ctatcggcag gttatgctgc tttattgaca
     9061 aatacaacag gaagttcaaa tgtggcctta ggttaccagt cattatattt caatactaca
     9121 gcatcatgga atacagccac gggttatatg accatgtata ataatacaac aggcagtgtg
     9181 aatgttgctt ccggatatag tgcattatat agtaatacta caggaacaaa caacgtagcc
     9241 ttgggctcaa gcgcgctgat aggtaacaca acaggagcgc gaaacgttgg tgttggctca
     9301 ggagcaggag atctcaatga caataataca aactgcacct ttttagggta tgatgcggat
     9361 caggctgtaa caacagattt tgataattca actgcactcg gcaatggctc aaggattact
     9421 gcaagcaatc aggtgcgtgt aggcaacgcc tcggtgactt caattggcgg ttatgttggt
     9481 tggacaaata tttctgatgg tcgagtgaaa aaagatgtca cagaaaatgt accggggtta
     9541 gcattcatca tggcacttaa acctgttacc tatcatctgg atattgatgc cattaacgac
     9601 catttacatg ttgcagattc tgttcaaaat cttcaatcta tcgccgcaaa aaacaaggtt
     9661 ttgtattcag gatttattgc acaggatgtt gctgctgctg ctaagcaaat aggttatgat
     9721 ttcagtggag ttgatgctcc taaatcggag gatgatcttt atggtttgcg ctatgccgac
     9781 ttcgttgtgc cgatggtaga ggcaatgcag gagcagcaaa agctcatcga gaagcaacag
     9841 gagcaaattg atgcattaac gaaattggtt caatccatgc aaaatcactg atatgcgaat
     9901 caccttatta ttatttggtt tgctatcatg tatagcagcg tcggcgcaat cgcggttgac
     9961 catgaatgac aatccatata ttgtaatgaa tggcagcgtg caaatggtaa ttgataataa
    10021 caatgccaac gcgattacaa caacagggag tggcgggaat attattagtg aatcagaaac
    10081 aaatattatc agctggcata ttaatacggc aaccggcagt tatatagtac cattcacaac
    10141 aagcgatggt gtaaaaattc cattaacagt gcaaattaca agtgcaggtg caggagcagg
    10201 agtaattgat ttttcaactt atggcggcct gacatggaat aatgacacct ataaaccggg
    10261 agaagtaacc aatatgacca atatgggtgt aaccaacaac tcgacagaga ttatagatcg
    10321 attttggatc atagatgcac atggatacac tacaaagcct tcaggaaata ttcaattcaa
    10381 ttatgatgat gcagaacatc ttgctgctgg aaatactatc aatgaaccgg atttaaaagc
    10441 tgagcgatat gaacccggta caaataactg ggaagtattt cctgttggag gaataataaa
    10501 cacaggaagc aattatgtta caggtgttcc atttaattcc ggcgatttta tgcgcacatg
    10561 gacgctaatt gatcaaacaa cacatttact tccaattgtg cttacaaatt tctcggcaac
    10621 ctgtgtagca ggaggtacaa atttacaatg gactacagct tctgaaataa actcagatca
    10681 ttttataata gcgggaagca ctgatggcat ttactttgaa gaccttgccg acatacaagc
    10741 tgcgggaaac agtaatacac caacatccta tagttacact gccgacgaca atatgttccg
    10801 gtattataaa ctttctttgc tgaatacgga tgcaacaatt gaagagctag gcattcttag
    10861 cactagctgc aaccagcaaa ttacaccgga attttttgcc tactcaccag cttctttcca
    10921 gattactgca caaactcagc agttgacacc cggaaattat gtaatatcga tatttacatt
    10981 gcaagggcaa ttaataaccg gcagcaattt acagttaggt acagaagtcg ccaccatgac
    11041 atattacgac caaagtttat ctgatggtat ttatattgta gcactcaggt caaccgacaa
    11101 tccaaaaatc acaatgactt caaaagtggc cttgtttcat taaattttcg attagaaaca
    11161 atatccattt tcggcaatca ttttatctgc aattcttctg cgtgctgcag tggtgttcac
    11221 tgtgcgatat ttcgtaaagc gtttcatccc gagtaacatc atgcgcagtt catcgccggt
    11281 agcaaaagat gcaatggcgg ttctgccttg tttattgaga cgatctatgc tatccgtaaa
    11341 atatacagca agcatatcgc ggtgtattga cgtggcatcc tcaccattct gaagggtgag
    11401 tttcttaatg cgcaacaaca tagattcagc agtaaaaatc tcattcatca tatcggcagc
    11461 acacattaat atttcctgtt catctttcaa cgccatcatc aatttttgca ctgctgcacc
    11521 tgcaaccatg aagaatgctt ttttcatatt ttctatagct ttctcttctt catagaacag
    11581 accttcacct gtttctccca attccggaat acccatcaat tctttttgca ctgccattgc
    11641 gggaccaagc atatccaatt gccctttcat gacgcgtttg agcaacatgt cgaccgttaa
    11701 tagtctattg atttcattcg tcccttcgaa tattctgtta atccttgcat cgcgatatgc
    11761 gcgtgcagcg ggatattctt cgctatagcc attcccacca tggatttgca cacactcatc
    11821 tactacaaaa tcgagcactt cactacctat taccttcaac atggcgcatt caatagcata
    11881 ctcttctgct gcacctagta gtgctttttc gtggccgata cctttcgaca ataattcctt
    11941 ttcttttttg tcgatgtaat cgctggtgcg ataggtagca cttaatgcag cgaaggtgcg
    12001 aatagcctgc tcactcaatt tatattttat tgctccaaac gaagcgatag gcacattaaa
    12061 ttgcatacgt tcatttgcat atttaactga tgtatcaatg agcgccttcg caccaccaag
    12121 tacgccaaca cataatttgt atctgcctat gttgaggata ttaaatgcaa tgagatgtcc
    12181 cttcccaatt tcccccaaca cattttcaac gggcacaggc gtattctcaa agaaaatctg
    12241 acatgttgaa gaacccttaa tacccatttt ttcttcttct acaccaatgg taaatccggg
    12301 ggcaccgcgc tcaacaatga aggcagtgaa tttatcacca tcaatttttg cgaacaccgt
    12361 aaatatatcc gcgaatcctg cattggtgat ccacatcttt tgtccgttga ggatataatg
    12421 tttgccgtct tcactcagca ctgcctttgt ttttgcagca agcgcatcac taccacttcc
    12481 aggttctgtg agacagtagc ttgcatgcgt agttccggca gccagtcccg gcaggtattt
    12541 tttcttctgt tcctctgtac cgaaatacaa gatcggcaaa gtaccgatgc cactttgtgc
    12601 cccgtaagca actgagaaag catgtgcagt acctgttact tccgtcacag cagagatagt
    12661 attaaaatct tttgcaaatc caccatacgc ctccggaatt ggcaaaccca gtattccaat
    12721 ctcaccggct ttcgtaagca atgcggccat taagccttcc tcatgcttgt cgatgcgatc
    12781 caaatttggc agcacctctt tctgcacaaa gtcggtggtc atctgcttga tcataagctg
    12841 ctcttcattc aattcctcag gaataaaaat gtctgacggg gtggattggt ggaggaggaa
    12901 tgagcctcct tttagtatga gattatttga tgacatataa ttaatttttt ggtatcaagt
    12961 atcaggtatc aagtatcagg tattggtatt tgccaaagat gattcgagat tatttagtta
    13021 tttttaagtt tgagtgattc tttgagttta aacaattttt tttggactaa tgatgtcttt
    13081 gataatagta attgagattc ctcattttta ataaggctta aactttcagc gatcattaat
    13141 tgcgtttcta gttcaaatga ggacgcatgt gcaattgaca agaaattatc aaagtcttta
    13201 tctgattttc ttccacaccc ttctgtaata tttgaaggaa tagaaatggc cgaccttctt
    13261 atttgttgca ccagtccata tatttcctcc ttagggaatg ttttagttgt taaataaact
    13321 tcctttacaa gctccatggc attctgccac acaattagtg atttgtagtt atgcataaat
    13381 aattattaag ggttaaacaa aatattttaa ttaatctctt ctaaactcta atatatacat
    13441 ataaatcccc tctttaaatt taccatccca ttattacctg atacctgata cctgatacct
    13501 gatacctgat acctgatact tgataccca
//
LOCUS       DAOQHY010000089        13339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2250_length_13339_cov_6.296823, whole genome shotgun sequence.
ACCESSION   DAOQHY010000089 DAOQHY010000000
VERSION     DAOQHY010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13339
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2250_length_13339_cov_6.296823"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="PLL28_08560"
     CDS             <1..1060
                     /locus_tag="PLL28_08560"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNF69412.1"
                     /translation="ISGSYTLINEAMNLNFWPRIAVRQPSDTKGQIYIPSVNIMLWIG
                     CVLVVLHFKTSSHMEAAYGLAITITMMVTTYLLSYYLAFKLRWPKLLIGLLVTLFASI
                     EISFFIANIRKFPEGGYITIMVSSIFILIMYTIYKGRKISNRFTKFVDLGKYAQQIKR
                     LSEDDEVPRFSTHLVYLTKANYRHEIEEKIIKSIFSKKPKRADVYWFLHIHRTEAPYT
                     LSYNVSELIEDKVIKVNIHVGFRIQPRTELYFKLIVQALIDNKELNLHLRDDGSTKYN
                     YEPDFKFVVIEKFLSVENEFSLNEGLLLNGYFFLKQLGQSDEKAFGLDKSDVVIEDVP
                     LVYQPVNQVQLTRLDNNA"
     gene            1071..1361
                     /locus_tag="PLL28_08565"
     CDS             1071..1361
                     /locus_tag="PLL28_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69413.1"
                     /translation="MSDTSLAFMHALAENHGRDVSISLLDGTVHSGEIVGYFKGDGVI
                     GTSYIYKWHLLPKGVDNPTGLDFWGVPAGEYILHKHIEKVVFLSDGFTLIMK"
     gene            1416..2756
                     /gene="xseA"
                     /locus_tag="PLL28_08570"
     CDS             1416..2756
                     /gene="xseA"
                     /locus_tag="PLL28_08570"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNF69414.1"
                     /translation="MSEQSGTKKIFSLSEVARSIEKTLSERYSSAFWVTAEMNKLNLY
                     KHSGHCYPELVEKKDGKIVAQFKSTLWRADYLRINAVFQDVLKEPLKDGIKILFLARI
                     TFDAAHGLSLSIIDIDPNYTLGDLEREKLETIAKLKAEDLYAKNKQIALPQLPQRIAI
                     ISVETSKGYVDFLKVIEQNPWGYKFFHMLFPSLLQGDKAIDSIIQQLMLIEKVKHHFD
                     IIAIIRGGGGDVGLSCYNNFRLASAIANSSLPVLTGIGHATNETVCEMIAHYNAITPT
                     KLGEFILQQFHNFSVPVNDAKKFIADKALRILMEERTHLKSEVKLFRSMTENLLLRHN
                     NDVQSNIKLIRQVMNFRLRSEKNNIDYLKQQIHMRSAAFVLERNRELVGIERNVINMS
                     PALVLKRGYTITLHKGAVIKSFEELQQGDAIETITSEGVINSAITSIKKNNNAG"
     gene            2746..2949
                     /gene="xseB"
                     /locus_tag="PLL28_08575"
     CDS             2746..2949
                     /gene="xseB"
                     /locus_tag="PLL28_08575"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNF69415.1"
                     /translation="MQDKIGYSEAFDELQKIVAEIELGEISVDELSAKVKRASELIRI
                     CKAKLTSTEEEVDKILKELLDGE"
     gene            3012..3935
                     /locus_tag="PLL28_08580"
     CDS             3012..3935
                     /locus_tag="PLL28_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNF69416.1"
                     /translation="MQTIIGSGGAIGVPLAKELSVYTKDIRLVSRNPKKVNETDQLYP
                     CDLKDTGAIEGAVAGSEIVYVTVGFEYNIKVWQAVWPPFMRAVVDACIKHKAKLVFFD
                     NIYSYDPKAVGMLTEDAPLNPSSKKGRVRKSLVDMINKEVAAGTLTAMIVRAADFYGP
                     AVNTSVLIEMAAKPMKAGKTANAMGNIDKIHTYTYTPDAGKATAVLGNTPDAYNQVWH
                     LPTTKEKLTTRQWMEMAAKEIGVQPKIRVAPMWMLKLMGWFVPVMREFPEMMYQFDND
                     YIFDSTKFEKRFGITATKPETGIKEMVAAMN"
     gene            complement(3974..5197)
                     /locus_tag="PLL28_08585"
     CDS             complement(3974..5197)
                     /locus_tag="PLL28_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69417.1"
                     /translation="MRRINIIILSAVIFTGLNSCDFLFGTREDAVVDEIFEEGAIDPE
                     LVQDEVGYVPVLPFWTDFIEPHDIFCGYDEMIYVVDAEGLKILDQTGTVFQTIAIPGA
                     TDVTQDRRLHTYVAGRVDYDVDGDGVMENLAAVYHLTGTSYGNVEIIDTLIHPFCDAS
                     RNITAFRGADDEAVEFTGLATLADNTLYVTRKGPRNDVSGIARADNAMLIYDENGNNT
                     SYANGLSPVTGNLKSVWDISGIATFAAPPQSLSGISGSAEFMICLDQPSAPYKGLWIT
                     QTVDPEAGIIYGENALLTVFDYTKADRFLYEPDRFIQPTDIYIAPDFTGYIFITDAGT
                     DSLYQFTRKGYEGVNPPAGSPNTKQIIASFGGVGSGPFNLIDPSGVAYLRKVVYVADK
                     GNDRICRYKLSSDLE"
     gene            complement(5199..6230)
                     /locus_tag="PLL28_08590"
     CDS             complement(5199..6230)
                     /locus_tag="PLL28_08590"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNF69418.1"
                     /translation="MRKLLLMVSLLWGLQPVGAQILPSYGDSRTATTGWQFLKIAPDG
                     RSAGMSEAFLAVVDDVSSLYWNAAGLTQLDSNRLHFGVQYTDYAAGTSMNFGGVSYRI
                     DDNTLIGVSMQYFDAGEMDVTTEFLPFGNGQTFRATDLGLGISLARELTDMFSLGITA
                     KYVREDLASVHNQNIVFDFGFQYDIGVQNTRFAVAITNFGFNTQPGGEIAVVTLSDSI
                     QATDFTEIAVPTVFRLGFAWDAIKNEKHILTTAFQLNHPTDNNETYSLGLEYSWKQLF
                     YARTGYTFATDRSALPAFGFGTYLHRNYGTIKFDYGFNYDAVLGMINKIGIAYTLPNG
                     IHKKSSATH"
     gene            complement(6383..7786)
                     /locus_tag="PLL28_08595"
     CDS             complement(6383..7786)
                     /locus_tag="PLL28_08595"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNF69419.1"
                     /translation="MKNLTSIIGICGVALITLLSSCKDEFLDKVPYDAIPFDDALLTE
                     DDMQAALNGVYSNMLGSGLYGRTVPIMGDLLADNVYISSANTGRYIPFNTYAIFDDNG
                     DVDGVWSSAYYAILNANQVISADLPANANVNQMRGEAYAARGMMYFDLVRIFAKPYNA
                     DNINSAGVPIVLTYQPEIKPARNTVGEVYAQAVSDLTTAISLMTEEKNSEYISKYVAV
                     GLLAKVYQYMGDWENAKTQALDLINNGGYSLVDSADFVSFWADPGPSELSETLFELRM
                     DIAVNNGFNSLAAMYDDAVYGDALVDSNFYNSYSDADVRKQLMFEAELSGEPIVRVNK
                     YPNSSNTSDYDDIKIIRLAEVYLILAEAYYHLGDEGNALTYLNAIAQQREPSFEGYTS
                     SGETLLNDITNERRKELAFEGNRVFDLNRLGLDINRGPQFPSDALFIPISDYRRLMPI
                     PLYEINANPAMVQNPGY"
     gene            complement(7813..10842)
                     /locus_tag="PLL28_08600"
     CDS             complement(7813..10842)
                     /locus_tag="PLL28_08600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HNF69420.1"
                     /translation="MYRHTILRLCICSIVLFHFSIAFGQVRTITGTTVDQQSGQPLPA
                     VSILIKNTQSGTVSDEYGKFSIEAKSGDTLTFNFIGYQPVLLAVSGTTNWTVSMQPEA
                     KMLSEVVVMAYGESSQRNQSGSVSKVSSKKLKEIPMASVDQILQGQVAGLQVNTYSGQ
                     PGGEINIHLRGVGSITAGSTPLFVVDGIPINSGVVGFGYNITWTSNGLAGINPNDIES
                     VSVLKDAAATAIYGSRAANGVIIITTKKGKAGKTKFELNSDYGFNTLAFNSNVRPLTA
                     DEFIDLTIIGMNNAGFGEVKQENILEDLHAYDGVNTNWLDEVTQNGMQNSTNFSVRGG
                     DEKTKFYTSFGYFNQEGNTIDSKFNRLSGSLNMEHAASSRLKFKTNILLSRSHQNTPY
                     GSGYFRAPIISGYLLVPTYSPYDTSGGFNLNNNELATFNPLAIASMDTNYYNNKKIIG
                     GFGADYEIIKGLTLSTQLSLDQNYTDEFQYANPFYGDGVALDGWASSAYTDITNWVWT
                     NLAKYNRMLMSNRLAMDITLGHEAQSSKQNTIYAFGQSLPPNLDIMVLSAAATPGDVF
                     SNIYEYTFESFFANTQFKYNDKYLLSVSVRRDGSSRFGINNRYGIFYSVGGSWNIDQE
                     DFFKGGGMVSALRLRASYGVNGNSDIGNYDAQANYLYTGSYNGVPSSAPVSTGNYDLT
                     WEKNKPFNVGIDASFFKDRFDLKADYYTRTTTDLLLNVPVSQTSGFSTQLQNLGAMIN
                     KGIEIELGADILRHRSVSWHIEANFSTNKNEITELYNHQQFISGYNNVYTAALYLYRE
                     GESYGTFYIPLYAGVDAQTGDAMWYTDSTMTETTTDVAEAKNAIAGNAQPKFFGGLNN
                     SVSWKGITLSAQINYVTGNQIYDIWGGFFQSDGANPQYNQYNTLLDSWQQPGDETDVP
                     KYVYYNKSGSNSTSTRFLYDGDYVRLRDVTLSYDIPANLATKLRMNSIRVYVRGTNLY
                     TWTKDKDLPFDPDVSYGYGDLNLPVMKSMIGGITLGF"
     gene            10982..11623
                     /locus_tag="PLL28_08605"
     CDS             10982..11623
                     /locus_tag="PLL28_08605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HNF69421.1"
                     /translation="MPEILPEALERYVAEHSTPESDVLSALNRQTYLKVMMPQMLSGH
                     VQGRVLSMLSHMIKPKRILEIGTFTGYSALCLAEGLQEGGKLTTIDINEELTPMVKQA
                     IENAGMQNKIDVVVGNALQIIPTLNETFDIVFIDADKQNYQKYFDLVINKVPVGGWIL
                     ADNVLWSGRVIEQKHDKDTAAIHAFNQYVSTHPRVDQVIFAVRDGISVCRRVG"
     gene            complement(12004..12165)
                     /locus_tag="PLL28_08610"
     CDS             complement(12004..12165)
                     /locus_tag="PLL28_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69422.1"
                     /translation="MHVQLGRDVDALYVGMVNALILDAKNIRDLLKFNRGVGGVGRVG
                     GVGGIIHVS"
     gene            12343..>13339
                     /locus_tag="PLL28_08615"
     CDS             12343..>13339
                     /locus_tag="PLL28_08615"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNF69423.1"
                     /translation="MFKSIIIAILLLPTISFAQFSFFGDTEPTGEPEMHYYKFYDGIQ
                     MIEFDYLTAYHKRVNYLEQAKPRALTWEMVLGPPHPNVYLFRNENGDIIKQYGSVDVS
                     KLLTVEESKKIAESHNSLFAKLGSVVGFSQISTRYFPYYLIGSVSNNSESALGLIDSF
                     GNVVLQPEYMVIWQHGDMFITRTDSINELRDKDLNIRFISNMYDLQPSYNHYGFADIR
                     LNDRWGLMDATGKIVVPCKYNMLVDAFNEYGLAQVSNVNGIGYVDSTGEEVIKCKYQS
                     VGEFREGLLDARYNDKWGYIDTKGNTIIPHKYDIGIWFEDGLARVAKREGSQYYFG"
BASE COUNT         3993 a         2755 c         2762 g         3829 t
ORIGIN      
        1 catcagcggt agttatacct tgataaacga ggctatgaac cttaacttct ggccacgaat
       61 cgctgtaagg caaccttcgg atactaaagg tcagatttat attcccagcg taaatatcat
      121 gctttggata ggatgcgtgc ttgtggtatt gcatttcaaa acatcatcgc atatggaagc
      181 agcatacgga cttgctatta ccattaccat gatggttaca acttatctgt tgtcttatta
      241 cctggcattt aaattgagat ggccgaaatt gctcatcgga ttgctggtta ctttatttgc
      301 ctctattgaa atttcattct ttatcgcgaa cattcgcaaa ttccctgagg gaggttatat
      361 cactattatg gtgagttcga tattcattct catcatgtac accatttata aaggaaggaa
      421 aatcagtaac agattcacca agtttgtcga cttgggtaaa tatgctcagc aaatcaaacg
      481 cttgagtgaa gatgatgaag tgccacgttt ctctacacat ttggtttatc ttacaaaagc
      541 aaattatcgt cacgagattg aagagaaaat tatcaagtct atcttttcca aaaaacctaa
      601 acgagccgat gtctattggt ttctgcacat tcaccgcact gaggcgcctt acaccttatc
      661 atacaatgta tcggagttga tagaagacaa agtgataaag gtaaatatac atgtaggatt
      721 caggattcaa cctcgaactg aattatattt taaactcatt gttcaggcgc tcatagacaa
      781 taaagaactc aatcttcatc tgcgtgatga tggttcgaca aagtataatt atgaaccgga
      841 tttcaagttc gtggtgatag aaaaattctt gtctgtagaa aatgaatttt cgctgaacga
      901 aggcctcctg ttgaatgggt atttcttcct caaacaactt ggtcaaagtg atgaaaaagc
      961 atttggcctc gataagagtg atgtcgtaat tgaagatgtt cccttggtgt atcaacctgt
     1021 gaatcaagtg cagttgacac gccttgataa taacgcttaa agtttcatat atgtctgata
     1081 cttcattggc atttatgcat gcactggctg aaaatcatgg tcgagatgtg tcaatttcgt
     1141 tgctcgacgg caccgtacat agtggagaaa ttgttgggta tttcaagggc gacggcgtta
     1201 ttggcacttc ttacatttat aaatggcatt tgcttcctaa aggggttgat aatcccacag
     1261 gcttggattt ctggggcgtg cctgcaggtg aatatattct acataaacat attgaaaagg
     1321 tggtatttct gtcagacggt tttacgctga taatgaaata acaatgtctg ccttacttgt
     1381 agggacaacg gcaaaattgt attttagtcg cccgcatgtc tgaacaatcc ggcactaaaa
     1441 aaatattctc tctttcggaa gtagcgcgca gtattgaaaa aacactgagc gaacgctatt
     1501 cttctgcttt ctgggtgacg gcggaaatga ataagctcaa tctttataag cactcaggtc
     1561 attgttatcc tgaactggta gaaaaaaaag acgggaaaat tgtagcacag tttaaatcga
     1621 ctttatggag ggctgattat ctgcgtatta atgcagtatt tcaggatgtg ctgaaagagc
     1681 ctttaaaaga tggcattaaa attttattcc tcgcacgtat caccttcgat gctgctcacg
     1741 gattgtcgct gtcgattatt gatatcgacc ccaactatac tttgggcgat ctggaacgtg
     1801 aaaagttgga aaccattgcc aaattaaaag cggaagatct ttatgcgaag aacaaacaaa
     1861 tagcgcttcc gcaactgcct caacgcattg ccatcatcag cgttgaaacg agtaaaggtt
     1921 atgtcgattt tctgaaagtg attgaacaaa atccatgggg atataaattc tttcacatgt
     1981 tgtttccctc tttattgcag ggcgacaagg ccatagacag cattattcag cagttaatgc
     2041 tcatagaaaa ggttaaacat cattttgata tcatcgccat tatcagaggt ggtggaggtg
     2101 atgtggggct ttcctgttat aataacttcc gacttgcatc tgcaattgct aatagttcat
     2161 tgccggtact cacgggaatt gggcacgcaa caaatgaaac tgtttgtgaa atgattgccc
     2221 attataatgc aataacaccg actaaacttg gagaatttat tttacagcag ttccacaatt
     2281 tttctgttcc tgtaaatgat gcgaagaaat ttattgcaga caaggcactg cgcatcttga
     2341 tggaagagcg cacgcatttg aaatcggaag tgaaattatt tcggtctatg acagagaacc
     2401 tcttacttcg acacaataat gatgtgcaaa gtaatattaa acttatacgg caagtcatga
     2461 attttcgact gcggtctgaa aaaaataata tcgactatct gaaacaacaa atacacatgc
     2521 gatcggcggc gtttgtgctg gagagaaatc gcgaattagt gggcattgaa cgaaatgtta
     2581 tcaatatgag cccggccctg gttttgaaac gcggatacac aataactttg cataaaggag
     2641 cggtgatcaa aagctttgaa gaacttcaac agggagatgc aattgaaact attacttcag
     2701 aaggtgtgat caatagtgca ataacttcaa taaaaaagaa taacaatgca ggataaaata
     2761 ggatatagcg aggcatttga tgaactgcaa aagattgtgg ctgagattga gctgggagaa
     2821 atttcggttg atgagctttc tgccaaggta aagcgcgcat ctgagttgat tcgaatttgc
     2881 aaagcgaaac tcacttccac agaagaggaa gtagataaaa ttctcaaaga attactcgat
     2941 ggcgaatagt aagtgtataa atagtcaatt tttgatacaa tattttcccg gttaatacgt
     3001 ctacagcaat tatgcaaacg ataataggtt caggaggcgc tattggtgtg cctttggcga
     3061 aggagctttc agtgtatacc aaagacattc gcctggtaag cagaaatcct aaaaaagtta
     3121 atgaaactga tcagctatat ccctgcgatc tgaaagatac aggtgccatt gaaggtgctg
     3181 ttgcaggaag tgaaattgtt tatgtaaccg tcggatttga gtataatata aaagtttggc
     3241 aagcggtgtg gccaccgttc atgcgcgcgg ttgttgatgc ttgtattaaa cataaagcca
     3301 agctggtttt tttcgataat atctacagtt atgaccccaa agcggttggc atgcttacag
     3361 aggatgcgcc tttaaatcct tcgagtaaaa aaggccgtgt aaggaaatcg cttgtagata
     3421 tgattaataa ggaggtggca gccggaacat taacagctat gatagttcgc gcagcagatt
     3481 tttatgggcc tgccgttaac accagcgtgc tcattgaaat ggctgcaaaa cccatgaagg
     3541 cgggcaaaac ggcaaatgca atgggcaata ttgataagat tcacacctat acttataccc
     3601 ctgatgccgg aaaagccact gctgttttag gcaatacgcc ggatgcatat aatcaggtgt
     3661 ggcatttgcc tacaacaaag gagaaactca ctacgcgtca atggatggaa atggcggcaa
     3721 aggaaattgg tgtacaacca aaaatcagag ttgcgccaat gtggatgctg aaattaatgg
     3781 gatggtttgt accggtaatg cgggagtttc ctgagatgat gtatcagttt gacaacgatt
     3841 acattttcga cagcacgaaa ttcgaaaagc gcttcggtat cactgcaaca aaacccgaaa
     3901 ctggcattaa agaaatggtg gcagcgatga attaattatt tgtcacaacg aaagtagtgt
     3961 taatacataa gcatcactcc aaatcactgc tcaacttgta gcgacaaatg cgatcattgc
     4021 ccttgtcggc aacataaact actttgcgga gataggctac tccactgggg tcaataagat
     4081 tgaaaggacc actgccaaca ccaccgaaac tggcaataat ctgcttcgta tttggtgagc
     4141 ctgcaggtgg atttacacct tcataccctt ttctcgtaaa ttgatataaa gaatctgttc
     4201 ccgcatcggt aatgaatata tagcccgtaa aatcaggtgc gatataaata tcggtgggct
     4261 gaataaaacg atcgggttcg tataaaaatc tgtcggcttt agtataatca aatactgtca
     4321 acaatgcatt ttcgccataa ataatgcctg cctcagggtc aacggtttgt gtaatccaaa
     4381 gtcctttata gggtgctgac ggctgatcaa gacaaatcat gaattcagcg gaacctgaaa
     4441 ttcctgaaag gctttgcggt ggtgctgcga aagtcgcaat tcctgaaata tcccatacgc
     4501 tttttaaatt acctgttacc ggtgataatc catttgcata actggtatta ttgccatttt
     4561 catcgtaaat gagcatagca ttatctgcac gtgcaatacc actcacatcg ttgcgaggtc
     4621 ctttgcgtgt tacatataaa gtattgtcgg ccaatgtggc taaacctgta aattccacag
     4681 cctcgtcgtc tgcacccctg aatgccgtga tgtttctcga tgcatcacaa aatggatgta
     4741 tcagggtatc aatgatttca acatttccat atgacgttcc tgttaaatga taaacagctg
     4801 caaggttttc catcacacca tcgccatcaa catcataatc tacgcgaccg gcaacataag
     4861 tatgtaaacg cctgtcttgt gtaacatcag ttgcgcccgg tatggcaatt gtttgaaata
     4921 cggttccggt ttgatcgagt atttttaatc cttcggcatc tactacataa atcatttcat
     4981 catacccgca aaaaatatca tgaggctcaa taaaatctgt ccagaagggt aataccggaa
     5041 catagccaac ttcatcttgt actaattcag gatcaatagc tccttcctca aaaatttcat
     5101 ctaccacagc atcttctcgt gtgccgaata aaaagtcgca actgtttaag ccggtaaaaa
     5161 ttactgctga taatatgata atattaatgc gacgcatatt aatgtgtggc tgaagatttt
     5221 ttatggatac cgttaggtaa tgtataagca attcctattt tattaatcat gcccaatacg
     5281 gcatcataat taaatccata atcgaattta atagtgccat aattcctatg caaatatgtt
     5341 ccgaatccaa atgcgggcaa cgcgctgcga tctgtggcga atgtgtatcc ggtgcgtgca
     5401 taaaacaatt gcttccagct atattccaaa cctaaactat aagtttcgtt attgtcggtt
     5461 gggtgattta gctgaaaggc agtagtgagt atatgctttt catttttaat tgcgtcccat
     5521 gcaaatccca atctgaaaac tgtcggcacg gcaatctcgg tgaaatctgt tgcttgtata
     5581 ctatcactga gtgtaacgac agcgatttca cctcccggct gtgtattaaa tccgaaatta
     5641 gtaatggcta cggcaaagcg tgtattttgt acaccgatat catattgaaa tccaaaatca
     5701 aaaacaatat tctgattgtg cacggaggca aggtcttcac gcacatactt tgctgttata
     5761 cctaagctga acatatcggt tagctctctt gcaagtgata tgccaagccc taagtcagtt
     5821 gcgcgaaatg tctgtccgtt gccaaagggc aaaaattcgg tggtaacatc catttcaccg
     5881 gcatcgaaat actgcatact cacacctatt aaggtattgt catcaatcct atagcttacg
     5941 cctccaaaat tcatgcttgt tcccgctgca taatcggtat actgaacgcc aaaatgcaat
     6001 cggttgctat ccagttgggt gagtccggcg gcattccagt acagggatga aacatcatct
     6061 acaacagcca gaaaagcttc gctcatgccc gccgaacgtc cgtccggagc tattttgagg
     6121 aattgccagc cggtagtagc cgtgcgcgaa tcgccgtagg atggcaatat ctgtgccccg
     6181 accggttgga ggccccaaag cagtgatacc atcaacaata gcttgcgcat agcggggcaa
     6241 agataataaa aggcttaggg agaccgatat agcctaaaac aggtttgttt tggagcccgg
     6301 aggtggtcaa ttgtcagaag ggataaaaaa agcgaaacac attacgcgtt tcgcttttac
     6361 acaaaatgga aagagctaca tactaatatc cggggttttg caccattgcc ggatttgcat
     6421 tgatttcata taatggaatc ggcattaatc tcctataatc tgaaatcgga ataaataatg
     6481 catctgatgg aaactgcggt ccccgattga tatccaaacc caggcgattg agatcaaaaa
     6541 cgcgattgcc ttcaaaagcg agttctttcc tcctttcatt ggtaatatca ttcaacagcg
     6601 tttcacctga cgatgtatat ccctcaaatg aaggttctcg ttgttgggct atagcattca
     6661 aatatgttag cgcattgcct tcatcaccta aatgataata agcctcggca agaattaaat
     6721 atacctcagc gagacgaata atttttatgt cgtcataatc agaagtattg gaagaattgg
     6781 gatatttatt tacacgaaca atgggttcac cgcttaattc agcttcaaac attaattgtt
     6841 tgcgtacatc ggcatcagag taactattgt agaaattcga atcaacaagc gcatcgccat
     6901 aaacagcatc atcatacata gcggcaagtg agttgaatcc attgttgaca gcaatatcca
     6961 tgcgcaactc gaaaagtgtt tccgataatt ctgatggtcc tggatctgcc cagaacgaaa
     7021 caaagtcagc agaatcgacc agtgaataac cgccattatt aatcaaatca agtgcctgcg
     7081 tctttgcatt ttcccagtct cccatgtatt gataaacttt tgccaacaaa ccaacagcaa
     7141 catatttact gatgtattca gaattttttt cttctgtcat taaactgatg gccgtagtga
     7201 gatcacttac tgcttgtgca tatacttcgc caaccgtatt tctcgcaggc ttaatttccg
     7261 gttgataggt aagcacgatc ggcacgccgg cactatttat attatcggca ttgtacggct
     7321 ttgcaaaaat gcgtaccaga tcaaaataca tcatacctcg cgcagcataa gcctcgccac
     7381 gcatttggtt aacatttgca ttggcgggta gatcagcact aattacttga ttggcattta
     7441 gaattgcata gtatgccgat gaccagacgc cgtctacatc gccattgtca tcaaaaatgg
     7501 cataagtatt aaacggaata tacctgcccg tattagcaga gctgatatac acattatcgg
     7561 caagcagatc gcccattatc ggcacggttc tgccataaag ccctgatcca agcatattgc
     7621 tgtaaacgcc attcagcgca gcctgcatat catcttctgt aagcagggcg tcatcaaacg
     7681 gaatggcatc gtatggcact ttgtctaaaa attcatcttt acatgacgat agcaaagtga
     7741 tcagtgctac gccacaaatt cctatgatag atgttagatt tttcattgta gtaaattttt
     7801 tctcgttgac aattagaatc caagtgttat tcctccgatc atcgatttca taacaggcaa
     7861 attaagatcg ccgtatccat aactcacatc cggatcaaat ggaagatctt tgtcttttgt
     7921 ccacgtatat aaattagtgc ctctaacata aacacgaata ctgttcatgc gcaatttggt
     7981 ggcgaggttt gccggaatat cataggataa agtaacatcg cgaagcctca cataatcgcc
     8041 atcatataag aatcgtgttg atgtggaatt ggagcccgat ttattgtaat aaacatactt
     8101 cggcacatct gtctcgtcgc cgggttgctg ccagctgtca agcagtgtgt tgtattggtt
     8161 atactgcgga ttggcgccat cactctgaaa aaatccaccc cagatatcat atatctgatt
     8221 gcctgtgaca taatttattt gtgccgaaag ggtgatgcct ttccatgaaa cactgttatt
     8281 caatccaccg aaaaattttg gttgcgcatt tcctgctatt gcatttttag cttcagcaac
     8341 atcggtagtt gtctcagtca ttgttgaatc tgtataccac atcgcatcgc cggtttgcgc
     8401 atccactccg gcatacagcg gaatatagaa tgtgccgtaa ctttctcctt cgcggtagag
     8461 atataatgca gcagtatata cattattgta accggaaata aattgctgat gattatacaa
     8521 ttccgtgatt tcatttttat tggttgagaa attagcttca atatgccagc ttacagaacg
     8581 atgcctcaga atatctgcac ccaattctat ttctatccct ttattaatca tagcgccaag
     8641 attctgcagc tgtgtagaga atcctgatgt ttgtgataca ggtacattca ataataaatc
     8701 ggtagttgtt cgggtgtaat aatcggcttt taaatcaaag cgatctttaa agaacgatgc
     8761 atcaatacca acattgaaag gcttgttctt ttcccatgtt aagtcatagt ttcctgtgct
     8821 taccggtgcg cttgaaggaa caccattata gctaccggta tataaataat ttgcctgtgc
     8881 atcataattg ccgatatcgg aattgccatt aacaccatag gaagcacgca aacgcaaagc
     8941 gctcaccatt cctccgcctt taaagaaatc ttcctggtcg atattccatg aaccgccaac
     9001 agaatagaaa atgccataac gattattaat accaaaacgc gatgagccat ctctgcgaac
     9061 acttacactc agcaaatatt tatcattgta tttgaattgc gtattcgcaa aaaatgattc
     9121 aaaggtgtat tcataaatat tgctgaaaac atctcccgga gttgctgccg cagaaagcac
     9181 cataatgtcg aggtttggag gcagactctg accaaatgca tatatggtat tttgtttcga
     9241 cgattgcgct tcgtggccaa gcgtgatatc catagccagc ctgtttgaca tcagcatgcg
     9301 attgtatttt gcaaggttag tccataccca atttgtaata tcggtatatg cagatgaagc
     9361 ccatccgtca agcgcaacac catcgccata aaagggattt gcatattgaa attcatccgt
     9421 ataattctga tcaagactga gttgggttga tagggttaat ccttttatta tttcataatc
     9481 agccccgaaa ccaccaatga tctttttatt attgtaataa ttcgtatcca ttgatgcgat
     9541 agccaaggga ttaaatgtgg caagttcatt attattaaga ttgaaaccac ctgaagtatc
     9601 atacggacta taagtgggca ccagtaaata tccgctgata ataggcgctc tgaaataccc
     9661 gctgccataa ggtgtattct gatgtgaacg cgacaaaaga atattggttt taaatttcaa
     9721 acgcgatgaa gctgcgtgtt ccatatttaa tgaacccgac aatcgattga atttagaatc
     9781 aatggtattg ccttcctgat tgaaataacc gaatgaggta tagaattttg ttttttcatc
     9841 gccaccgcgc acagagaagt tggtggaatt ctgcatacca ttttgtgtca cttcatcgag
     9901 ccagttggta tttacgccat cataggcatg caagtcttca agaatgtttt cctgtttcac
     9961 ttcaccaaat ccggcattgt tcattccgat aatagtgaga tcaataaatt catctgcagt
    10021 tagtggtcga acgttgctat taaaggcaag tgtattaaat ccgtaatcgc tatttaattc
    10081 gaatttcgtt ttaccggctt ttcctttttt tgtagtgatg atgattacgc cattcgcagc
    10141 gcgtgatccg taaatcgcgg tagctgcggc atcttttaat acgcttacac tttctatgtc
    10201 attcggattt attcccgcta atccgtttga tgtccatgta atattatagc cgaagccaac
    10261 aacgccgctg ttaatgggaa tgccatccac cacaaataat ggtgtcgatc ctgcggtgat
    10321 ggagcctaca cctcgcaagt gaatatttat ttcacctccg ggctgaccgc tataagtatt
    10381 tacttgaagt ccggcaactt gaccttgcaa aatctgatca acagaagcca tgggtatctc
    10441 tttcaatttt tttgacgaca ctttgctcac agatcctgat tgattgcgct gagatgattc
    10501 gccataggcc attacaacaa cctcactgag catcttcgcc tccggttgca tggataccgt
    10561 ccaattcgtt gttcccgaaa cagctagtaa aacaggttga tatccgatga agttaaatgt
    10621 cagcgtgtcg cccgatttag cttcaatgga aaactttccg tattcatctg aaacggttcc
    10681 gctttgagtg tttttaatga ggatggaaac agcgggaagt ggctgtcccg attgctgatc
    10741 aacagtagtt cctgtaatcg tgcgcacctg accgaaggct atcgaaaaat gaaatagcac
    10801 aatgctgcag atgcacaaac gcaaaatggt gtgtctgtac atagtaatta ggtttagtct
    10861 taaatgccgg tgagaggagt caccgatact aagatactta taatgccaaa tacattgtca
    10921 catatatata atacttgcaa tgtgtgatga aaatctgaca tttgaactat tactttgtac
    10981 tatgccagaa atacttccgg aagctttaga acgctacgta gcagagcaca gcacacctga
    11041 atctgatgtg ctttctgcat tgaaccgaca aacttaccta aaggtcatga tgcctcaaat
    11101 gttgagtggt catgtacagg gtagggtgtt gagtatgctc agtcacatga taaaaccaaa
    11161 gcgcattctt gagatcggca catttaccgg atatagtgca ctgtgtcttg cggagggatt
    11221 gcaggaaggt ggtaagctga caaccatcga tatcaatgaa gaacttacac ccatggtaaa
    11281 gcaagcaata gaaaatgccg gcatgcaaaa taaaatcgat gtggtggtag gcaatgcatt
    11341 gcagataatc cctacattaa atgaaacatt tgatatagtg tttattgatg ccgataaaca
    11401 gaattaccaa aaatattttg atctcgtaat aaataaagtt cccgttggcg gatggatact
    11461 tgccgataat gtgttgtgga gtggaagggt aattgaacag aagcacgata aagataccgc
    11521 ggctattcat gcattcaacc aatatgtaag cacacatccc cgggtagacc aggtgatttt
    11581 cgcggtgagg gatgggatta gtgtttgcag gcgagtgggt tgatggggat tttcatgtgg
    11641 atggggttcg tcatcgtgaa tcgtgagtcg tgaaccagcc tcgccggcta ggcgggtcgt
    11701 gagtgaggtg cggaggttgt atcagcggat gaaattttct acattcacca ctcaccactc
    11761 accactcgcc actcaccact cacccactgc cagtgccgca tgatttcatc gtcatgacac
    11821 gtaaatcatc cctccaactc caaaaactcc gctaactccg ccaactccgc gattaaattt
    11881 cagtaggtca tgatacatga attatccctc caactccgcg attaaatttc agtaagtcat
    11941 gacacatgaa ttatccctcc aactctgcca actccgccaa ctccgcgatt aaatttcagt
    12001 agatcatgat acatgaatta tccctccaac tcctccaact ctgccaactc cgccaactcc
    12061 gcgattaaat ttcagtaagt cgcgaatgtt tttcgcatct aatatcaacg cattaaccat
    12121 tcccacatag agcgcatcca catcccgccc cagctgtaca tgcacacttg catactttca
    12181 cactttcaca cttgcacact tgcacacttg aaaaaccagc tccctgatac taaccatcaa
    12241 cctttatgaa gaaagatttt ttacgatttt taacggcaaa ataattagat ttggtaggat
    12301 tattgctgct aagttgctta aataaatccc acttcatcgc ccatgttcaa atcaatcata
    12361 atcgcaattc tcctcctccc aaccatttca tttgctcagt tctctttctt cggcgatacc
    12421 gagccaaccg gtgagccgga gatgcattat tacaagttct atgatgggat ccaaatgata
    12481 gaatttgatt acctaactgc ttaccacaag cgtgtaaact atctggagca agcgaagcca
    12541 agggctttga catgggagat ggtgctaggg ccgccgcatc cgaatgtata tctgtttaga
    12601 aatgagaacg gtgatatcat aaagcaatat ggtagcgtgg atgtatctaa gctgcttacc
    12661 gttgaagaat caaagaaaat agccgaatcg cacaattcct tatttgcgaa gctgggatct
    12721 gtggttgggt tttcacagat cagtacacgt tatttcccct actatttaat tggcagcgtt
    12781 tccaataact ccgaaagtgc cttgggattg atagatagtt tcggcaatgt tgttttacaa
    12841 ccggaatata tggttatttg gcagcatggc gacatgttta taaccagaac cgattcgatc
    12901 aatgaattga gagacaagga tctcaacatt agattcatca gcaacatgta tgatctgcag
    12961 ccatcttata atcattacgg atttgctgat atcaggttga acgatagatg ggggttaatg
    13021 gatgctaccg ggaaaatagt agttccctgc aaatacaaca tgttagttga tgcatttaac
    13081 gaatatggtc tggcacaggt aagcaatgtc aatggtattg gttatgtaga ctcaaccgga
    13141 gaggaggtaa tcaagtgtaa atatcaaagc gtcggcgaat tcagagaagg cttattggac
    13201 gcacgctaca acgataaatg gggttatatc gatacaaagg gaaatacaat catcccacat
    13261 aaatacgaca tcggtatttg gttcgaggat ggcctggcgc gtgtagcaaa gcgagaagga
    13321 agtcaatact atttcggtt
//
LOCUS       DAOQHY010000090        13337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2252_length_13337_cov_5.066406, whole genome shotgun sequence.
ACCESSION   DAOQHY010000090 DAOQHY010000000
VERSION     DAOQHY010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13337
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2252_length_13337_cov_5.066406"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..636
                     /locus_tag="PLL28_08620"
     CDS             142..636
                     /locus_tag="PLL28_08620"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNF69424.1"
                     /translation="MKKFVLLSATLFAAITMVNAQDKLPSLELSDVNGNKINVADYSN
                     DSTIQVFSFWATWCVPCKEELNNIAEIYEDWQKDYKVEIIAVSIDDAKTKANAKSYAE
                     GQGWTYTVLLDTNKDLQRLLGGQSVPFTVITDKSGNIVDKHTGYIEGDEYELEDKIAK
                     LAKE"
     gene            complement(667..1815)
                     /gene="hemW"
                     /locus_tag="PLL28_08625"
     CDS             complement(667..1815)
                     /gene="hemW"
                     /locus_tag="PLL28_08625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNF69425.1"
                     /translation="MYINDQEAGIYLHIPFCRKACHYCNFHFSTSLQSKEAYLLAMHS
                     EIDARVGYTEGKIIRSIYFGGGTPSVLTADEISSLLAHIKQLHTVRADAEVTLEANPD
                     DLSKPYIESIHAAGINRLSIGVQSFIDHDLQWMNRTHTGSQAKQVITDAMLSGFEHMS
                     ADLIYGIPGSSHEEWRSNIQTLIDLKVPHISSYALTVEPKTALAHFIQTGKSEMPDEQ
                     LAAEQFEILRHMLIDAGYDHYEISNFALPGHTAIHNSAYWAGYYYLGIGAAAHSYNGV
                     SRQWNINNNALYIRYDKSGNEYYTIENLDMHQRMNERIMTALRTTRGLDLERFLSEFG
                     EVYHNQLTNEIRRLESAEKMIIENNIARISPSHLFIADSVISDLFFVD"
     gene            1886..3646
                     /locus_tag="PLL28_08630"
     CDS             1886..3646
                     /locus_tag="PLL28_08630"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNF69426.1"
                     /translation="MNFFLWHHAHAQDSIYIVTNYNNQLNEYLSDFNTTFATIQEAEH
                     ALNDLVHTMQIDGYLTAAWRWEPNEMHRNPSVKVVEINLGKKVLWANLDASGVEPWLL
                     RAAGFDPEDWINTTYNQNKAASFLQELVSYANNNGYPFASSHLDSITYIDSTLSAKVI
                     FEKNILIFFDTIKVIGSLQVTSNFITQYTQIISGQPYDQSLIDALPSRLKELNFLQQT
                     KAPQIEFMGNKATVLIFADDKRSGSFDFLLGVLPNNEITGRLIITGDMRLHLQNVFHS
                     GEEIHFQYTKLESASKSMDAAFTYPYLPHIPLGPDASFHLYLKDSTFLERKASAGVVW
                     QIAGNHSLKGFTSFYNSSVLRPDTTFVLLNYALPSMLDVKENAYGLTWQYQHLDYTFN
                     PRKGYAIALTGTAGTRTILENVSITELFNPLTPDFDFSTLYDSIENKSLSLKYQYDLQ
                     YFIPLYNRMAILLQVRGASILHDNIVENELYRIGGNQLLRGFDEQSLPVSDYHIGTFE
                     YRYLLSQNAYTSVFVDAGVTKNNALDNTALVYPYGFGAGVRFETKAGIFGLSYALGGS
                     NMNPVQVKNTKVHFGYINYF"
     gene            3646..4536
                     /locus_tag="PLL28_08635"
     CDS             3646..4536
                     /locus_tag="PLL28_08635"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HNF69427.1"
                     /translation="MKRITVAACLMVSLSVTAGAQDTILQNPAPDTLIHYAYDTAALM
                     KRMHFAPVDYDSLLASPNTNRKTAPFSSFHVEKRDLLLPGLCLLMLIYVTWLRYTYGK
                     ELRENFTVILNTNLGQQIYRDREFSANIFKFLTFINFALGGGMLVYLTTTYYHVNLPF
                     NQTGLNVALMVMAVFALYLVKGFVYRFVGAVFNITASLRFFRFNALVIYHLLGIGLLP
                     FILLAAFAEPPVNTWALFAALILVAIAIVLRVFKGLTVALSSGKLHILYFLLYICALE
                     VAPLLIAIRLFDLWAGSQPK"
     gene            4544..5326
                     /locus_tag="PLL28_08640"
     CDS             4544..5326
                     /locus_tag="PLL28_08640"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNF69428.1"
                     /translation="MNKPQPTSLKPVKSILVSQPKPPEVPKSPYQEIAEKYKIKVEFR
                     QFIQVRGLSSKDFRKQRINILDYTAVIFTSRNAIDHFFRICEELRIRMPEMTKYFCVT
                     EAIAVYLQKYIQYRKRKVFFGTGKDTDMFDLFKKHISEKFLFPCTVNHKDIYTEYLKS
                     INADFSEAFMYETVPADLSDLKGKIDYDMIILYTPTGVASLFQNFPGFEQGDLRIGCM
                     GASTLKAMEEQNLRVDLKAPSHDSPSITMALDKYLAISNKKK"
     gene            5573..7627
                     /locus_tag="PLL28_08645"
     CDS             5573..7627
                     /locus_tag="PLL28_08645"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNF69429.1"
                     /translation="MPTNKLINETSPYLLQHAHNPVDWYPWGEEALEKARTEDKPILV
                     SIGYAACHWCHVMEHESFEDDTTAELMNKYFINIKVDREERPDLDNIYMNACQILTGA
                     GGWPLNVFLTPELKPFNAGTYFPPKPGYGKPSWKELLMYMYQVYADDRDKVEHQANLI
                     MEHIAKMDGAFLEKNFVLESENIFSKQQLDAAINTIQSNFDRVDGGFGSAPKFPATMT
                     LRFLLRYAWFTNNKPILDHVILSLNKMHQGGIFDQIGGGFARYATDNKWMIPHFEKML
                     YDNALLCSLYAEAYRATGDNNYLQVVKDIIQYIDRECSDELGGLYAAYDADSEGVEGL
                     YYTFTYQDLENALGDDAVWAAKVFNATSAGNWEHTNILHRDASDETTARQLDMTHEAF
                     SAKLKLVKGKLLQYREKRIKPALDNKILLSWNALMCSAFVDAYKASGEEQFRQKAIQL
                     VQHLTSAFKRKNTENAYWHVITNNIAKQTAFLDDYATLIQALFDVFEITGDEAWFKQA
                     DGCMEYVIANFSATDGMFFFTEKSQTDLPSRNAEYYDNATPSGNSIMLSNFQRRSIML
                     GNTKDVERVRTMLGAMKNSLIKYPTSFGNWWLSALKQIYPSVEIAVTGPDHQVMRDRI
                     FTHYHPHILVQNGNNAVSEFPLLQHRASDVETRIFVCHNFTCKKPVTTLQDFVSEVQT
                     LG"
     gene            7692..8690
                     /locus_tag="PLL28_08650"
     CDS             7692..8690
                     /locus_tag="PLL28_08650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HNF69430.1"
                     /translation="MKQIYARITCGLVFSVAVFNAQAQLISYGQQDPQFTQYMLNTSG
                     FNPAAIGVEDAWVSTLGVRSQWVSLPGAPVSQSLTSHAPIYKINSAVGVTVINDIMGQ
                     QRTTGASLDYAYHKRFGPSTLSIGASGGIFQETINGAKLVTPTGNYEGGVNHNDPNLP
                     VTTISKMIPDAAFGMYFFGRNLTAGISANHLLLPITAGNDANSGTVQYNPNGYVYVAY
                     GFDAGNRFRIMPNVLYKTDLNEGVVDLNTLVTYNNNIQAGISCRANTALAFDAVALMA
                     GMRVADRWLLSYSYDVTLSALNTVSSGSHEIVVRYSIPVARPRAGKMINNPRFLYH"
     gene            8838..10121
                     /locus_tag="PLL28_08655"
     CDS             8838..10121
                     /locus_tag="PLL28_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNF69431.1"
                     /translation="MKRNLQLFTLAFVAIIVGSGCSHSSWDDYRGQLLGMQDRPEWEP
                     VNPYGMVYIPSGTLHIGPSDQDVNNSLWARAKSISIQGFYMDDAEITNNEYRQFVYWV
                     RDSIMHTLLDHYVEEEEGMESEVPLIDWSQEIDWDDEETRSRLEELYLPASETYYGIK
                     AIDTRQLQYNYRYVKWKEAASKSNRDKNIAEFIVEEPIEIYPDTLVWVHDYTYSYNEP
                     MTRQYFSHPAFDDYPVVGVSWKQAGAFATWRSDFWNDWRRAHEEVQVDDFRLPTEHEW
                     EYAARGGRDMAPYPWGGPYVRNTKGCYLANFKPGRGNYPEDGGYYTVKVYSYAPNDFG
                     LYNMAGNVSEWTSSAFQENTYSFLHDENPDYRYDAEDSDPPALKRKVLRGGSWKDISY
                     YIQTGSRHWEFQDTAKCYIGFRCCMTFLGRSMSDF"
     gene            10206..11030
                     /gene="gldL"
                     /locus_tag="PLL28_08660"
     CDS             10206..11030
                     /gene="gldL"
                     /locus_tag="PLL28_08660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HNF69432.1"
                     /translation="MSAFFESDRFKRIKNLIIGIGASVVLVGALFKILHWPGADQMLM
                     VGMLTEAFLFLMLGIIPPHKDYYWEKIYPDLDIAPEVEEAKSGKKVHKMTEPKESVTR
                     QLDKMLEDSKVEPVMIERLGENLKKLGENIEKLSDVQDAAVNTNEYSNKIKDASTAVA
                     EMRDAYANAARAMEQITASSVDTSKYHEQVQMVTKNLSQLNAIYELELQDTNNHLKAM
                     NKFYGSLNSAMDNLQESLTDTSKYREEIAQLSNNLAALNRVYGNMLSAMSMGAARS"
     gene            11079..12683
                     /gene="gldM"
                     /locus_tag="PLL28_08665"
     CDS             11079..12683
                     /gene="gldM"
                     /locus_tag="PLL28_08665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HNF69433.1"
                     /translation="MSIPKEPRQLMINLMYLVLTALLALNVSAEILNAFNTVNNGISS
                     SNELLTSKNELMMTMISKAAAKDARPETQEILRQATEAKKMSDEFVKYVEGLKEEVKA
                     RAGGPNPDHPEKLKTEGEMEKTSRLFLNEGKGKELEAKINDAREKFLNVLGKESGIDV
                     PLRIVEVPEGEHATNWEEYNFDRVPAIAVMTILTKFQQDAKSSESTILDYLRRKINAE
                     DIQVDKMAAMVVAPSSYVRKGSEYTADIFIGATSKNIKPEVYVGTFTSAVKKDPADPT
                     GTAFLEVEGKDIPLNGARPIDMVNGIGKIKEGANGQRNFQGVIKIPHPSKEGEFRFYP
                     FEAGYETFEVGKAVISPTAMNVLYIGVDNPIKISVPGYASEKVSASGCGVTKVKGEEY
                     VARPSKVGEEEIVVSVQGNKGVEKSSEKFRIRRVPDPYVYILNTKGGPMKIGEFKAAD
                     GIIVKNPDFVFQVPYTVTGFEMLYAPKGSGGVSDMSNSSKFSSTMKDIQKKCKPGDTI
                     VLNDVAVKMPDGRTVKISASFKLIGG"
     gene            12704..>13337
                     /gene="gldN"
                     /locus_tag="PLL28_08670"
     CDS             12704..>13337
                     /gene="gldN"
                     /locus_tag="PLL28_08670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HNF69434.1"
                     /translation="MNRTLLILGLVLVLGTAVHAQPTSPAKLDGIYKRELLGTREIIP
                     YDDIREADIYWEKRIWRNIDFREKINLPFTWPVDPFVQVVYDAVMSGELTAYSPLYDD
                     FREGTAIPTDELKARFNKVDTTWILDETTYEEVPVIVVNEFDYKTVQKIQIKEDWVFD
                     EETSTMVVRIIGLSMIRDRIDPTTGEVITTETMFWLYYPDLRNVLIRHEVF"
BASE COUNT         3950 a         2818 c         2829 g         3740 t
ORIGIN      
        1 gctggatacc ggtaatgttc agacttcgtc gttttaatgt catttcctcg gtcattgccc
       61 gcaaggaggc taaaacctaa ggaaaattgg cacatatctt gctctgcaaa tgctatcttt
      121 gaaacaaaat atacccttgc gatgaaaaaa tttgttctgc tttctgccac cttgtttgct
      181 gctattacta tggttaatgc tcaggataaa ctcccaagcc ttgaactttc tgatgttaat
      241 ggcaataaaa tcaatgtggc cgattatagt aatgacagca ctattcaggt gttcagcttc
      301 tgggctacct ggtgtgtgcc ttgtaaggag gagttgaata atattgctga aatttatgaa
      361 gactggcaaa aagattacaa ggttgaaatc atcgcagttt ctatcgacga tgccaaaacc
      421 aaggcaaatg caaaatctta tgccgaagga caaggttgga cttatacagt gcttctcgat
      481 accaataaag accttcagcg tttattgggc ggtcaatccg tgccatttac cgtgatcacc
      541 gataaaagcg ggaatatcgt tgataaacat accggctata tcgagggtga tgaatatgaa
      601 ttggaagata aaatcgccaa actcgccaaa gaataaggca gcttctttag caccattcaa
      661 ttatttttag tcaacaaaaa acaaatcgct gatcacactg tcggctatga ataagtgtga
      721 tggagaaata cgtgcaatat tattttcaat aatcattttc tctgcacttt ccaatcggcg
      781 aatttcattt gtcagttgat tatgatatac ttcaccaaat tcgctcaaaa accgctccag
      841 gtctaagcca cgtgttgttc gcagggctgt catgatccgc tcgttcatgc gctgatgcat
      901 gtctaaattt tctatggtgt agtattcgtt tcccgattta tcgtatcgta tatataaggc
      961 attgttgttg atattccatt gtcgcgaaac accattatac gaatgtgctg cagctccaat
     1021 tcctaaataa taatatcctg cccaataggc gctattgtgt atggcagtat gaccgggtaa
     1081 tgcaaagttg gaaatttcat aatgatcata tcccgcatca atcaacatgt ggcgaagaat
     1141 ctcaaattgc tctgcagcca attgctcatc aggcatttcg gattttccgg tttgaatgaa
     1201 atgggcaaga gcagtctttg gttcaacggt caacgcatag ctcgaaatat gtggcacctt
     1261 caagtctatc aatgtttgaa tgttggaccg ccattcctca tgcgatgatc cggggatacc
     1321 ataaatgagg tctgccgaca tatgctcgaa cccacttaac atggcatcgg taataacctg
     1381 ctttgcctgt gaccccgtat gagtgcgatt catccattga aggtcgtggt caataaatga
     1441 ttgtacgcca atgcttaatc gattaatacc ggctgcatga atggactcaa tatagggttt
     1501 agaaagatca tcgggattcg cctccagtgt aacctccgca tcagcgcgaa cggtatggag
     1561 ctgcttgata tgtgcaagca gggaactgat ttcatcagcc gtcaacacag agggtgtgcc
     1621 tcctccaaag tagatcgatc gaatgatctt tccttcggta taccctacac gcgcatctat
     1681 ctcactgtgc atggcaagga ggtatgcctc ttttgactgc agggaggttg agaaatgaaa
     1741 attgcaataa tgacaggcct ttctgcaaaa agggatatgc aaataaatac cggcctcctg
     1801 atcgttaatg tacatgcgct tactgcaaaa gtatgggtaa atttgcataa ccattaccat
     1861 gaggcgattc ggtatactcc tctgcgtgaa tttttttctt tggcatcatg cacatgctca
     1921 ggatagtata tatattgtca caaactacaa caatcaactt aacgaatatc tgtccgattt
     1981 caataccact tttgctacta tacaggaagc agaacatgca ttgaatgatt tggtgcacac
     2041 gatgcaaata gacggctacc tcactgctgc atggcgatgg gaaccaaatg aaatgcaccg
     2101 taacccttct gtaaaagtcg tagaaataaa ccttggcaaa aaagtgctgt gggctaatct
     2161 ggatgcttca ggtgtagagc cttggctttt gcgcgctgca ggttttgatc ccgaagattg
     2221 gataaatact acttataatc aaaataaggc agcatcattt ttacaggaat tggtatccta
     2281 tgcaaacaac aatggttatc cgtttgcatc atcgcatctc gacagtataa catacatcga
     2341 ctcaacatta tcggcaaagg tgatatttga gaaaaatatt ctcatctttt ttgataccat
     2401 taaggtaata ggatcattac aagtaacatc aaactttatc acacaatata ctcaaataat
     2461 ttccggtcaa ccttatgatc aatcactgat cgatgcactg ccttcgcgtt tgaaagaact
     2521 gaattttctt cagcaaacaa aagcccccca aattgagttc atgggaaata aagccactgt
     2581 tctcatattc gctgatgaca agcgttccgg cagttttgac tttcttcttg gtgtgctgcc
     2641 gaataatgaa attacaggac gattgatcat tacgggtgat atgcgtttac atcttcaaaa
     2701 cgtgtttcat tcaggcgagg agattcattt ccaatataca aaactcgaaa gtgcttcaaa
     2761 gtcaatggac gcggcattta cctatccata tttaccgcat ataccgcttg gccctgatgc
     2821 ctcgtttcat ctgtatttaa aggattcgac attcctcgaa agaaaagctt ctgcaggcgt
     2881 tgtttggcag atagcaggaa atcattcatt aaaaggtttt acttcgtttt ataattcttc
     2941 cgtactcaga ccggatacca cttttgtatt gttgaattat gcattgccat ctatgctcga
     3001 cgtgaaagag aatgcttatg gcctcacctg gcaatatcaa catctcgatt atacatttaa
     3061 tccgcgaaag ggatacgcca ttgcattgac aggcaccgcc ggaacgcgga ctatcctcga
     3121 aaatgtaagc attacagaat tgtttaatcc cttaactcct gatttcgatt tcagtacatt
     3181 atatgatagc attgaaaata aatccctgtc gctgaaatat caatatgatc tgcaatattt
     3241 tattcccttg tataatcgta tggcgatttt attacaggtg cgcggagcat cgatcttaca
     3301 cgataatatt gttgagaatg aattatatag aatcggcgga aatcaattgc taagaggttt
     3361 tgatgaacaa agcttgccgg tctccgacta tcatatcggc accttcgaat atcgatacct
     3421 gctgtctcaa aatgcctata catcggtttt tgtcgatgcc ggcgttacca aaaataatgc
     3481 actcgataat actgctttgg tctatcccta cggtttcggc gccggcgttc gatttgaaac
     3541 aaaagcgggt atctttggcc tttcatatgc actcggaggc tcaaatatga atcctgtgca
     3601 ggtgaaaaat acaaaagtgc atttcggata catcaattat ttctagtgaa gcgaattacg
     3661 gtagctgcat gcttaatggt ctccctcagt gttacagccg gggcacagga caccattttg
     3721 caaaatcccg ctcccgatac cctcattcac tacgcttatg atactgctgc tttgatgaaa
     3781 cgcatgcatt ttgcaccggt tgattacgat agcctcctcg catcaccaaa caccaatcgc
     3841 aaaacagcac ctttcagcag ctttcatgtc gaaaaacgcg atcttctgct tcccggattg
     3901 tgcctgctga tgctaattta tgttacctgg ctaagatata cctatggcaa ggaattgcgt
     3961 gagaatttta ctgtcatatt aaataccaac ctcggacaac agatttaccg cgatagagag
     4021 ttttcggcca acatttttaa gtttctcacc ttcatcaatt ttgccctggg aggaggtatg
     4081 ctggtctact tgaccacaac atattatcat gtgaaccttc cctttaacca gacagggtta
     4141 aatgtcgcac ttatggttat ggctgtattt gcattatatc tggtgaaggg ctttgtttat
     4201 aggtttgttg gtgctgtatt caatatcacg gcatccctta gatttttcag attcaatgct
     4261 ttggtgattt accacctgct tggaatagga ctgcttccct tcatccttct ggctgccttt
     4321 gcagaacctc ctgtcaatac ctgggcactg tttgcagctt tgatattggt tgccattgcc
     4381 attgttttga gggtgtttaa aggccttacg gttgccctga gttcggggaa attgcatatc
     4441 ctctactttt tgctgtatat ttgcgccctc gaagtggccc cactactcat agccataagg
     4501 ctgtttgacc tttgggccgg ttcacagcct aaataagtaa ccgatgaaca aaccgcagcc
     4561 aaccagtttg aaaccggtaa aatctattct cgtttcccag ccaaaaccgc ctgaggtgcc
     4621 taaatcacca tatcaggaga ttgctgaaaa atataagatt aaagttgagt tccgtcagtt
     4681 tattcaggtg cgcggactgt cgtcgaaaga cttcagaaag caacgtatca acatactcga
     4741 ttacactgca gtcattttca ccagccgcaa tgctatcgat catttcttcc gcatttgtga
     4801 agaactgaga atacgcatgc cggaaatgac aaaatatttt tgcgttaccg aggctattgc
     4861 cgtttacctt cagaaatata tacaatatcg taaacgcaag gtattcttcg gcacaggaaa
     4921 agacacggat atgttcgatc tgttcaaaaa acatatcagc gaaaagttcc tctttccttg
     4981 tacagtcaac cacaaagaca tctataccga atatctcaaa tctattaatg ccgatttctc
     5041 agaagcattc atgtatgaaa cggttcccgc cgatctttct gatttgaaag ggaaaataga
     5101 ttatgatatg atcatcctct atactccaac cggtgttgca agcctctttc aaaacttccc
     5161 cggattcgag caaggcgatt tgcgcattgg ttgtatgggc gcatccacac tcaaggctat
     5221 ggaagaacaa aatcttcgcg tagatctcaa agcgcctagt catgattctc cttccattac
     5281 tatggcattg gataaatatc tcgccatcag taataaaaag aagtaagtaa tttattccaa
     5341 cactccttgc ctttacatgc gaacctattc gaataggtca gcttgcttat tttttatggc
     5401 cgatgtaact ttaatttctt aggaattaac ggcatacaaa ctttgtaaat acctttattg
     5461 ttaatccgat tgattgtaat tgccattcat gtgtaaggaa ggcgaaaagt ttttattcct
     5521 ccaacctgag atgcaatcgg aaaacctagt aaatacctaa attaatattc gtatgccaac
     5581 aaataaactc atcaatgaaa cttcgcccta tctgctgcaa catgcacaca atcctgttga
     5641 ttggtatcca tggggtgaag aagcattaga aaaagcaaga acagaagata aacccattct
     5701 tgtttctatc ggttacgcgg cctgccattg gtgtcatgtg atggaacacg aaagttttga
     5761 agatgatacc accgctgaat tgatgaataa atactttatc aatattaaag tagacagaga
     5821 agaacgcccc gatctggata atatttacat gaatgcctgc cagatactca ccggtgccgg
     5881 aggatggccg ctgaacgttt tccttacgcc tgaattaaaa ccatttaacg ccggaaccta
     5941 tttcccccct aagccgggct atggcaaacc ttcttggaaa gaattgttga tgtatatgta
     6001 tcaggtttat gctgacgatc gcgataaagt agagcatcag gcaaatctga tcatggaaca
     6061 tatcgctaaa atggatggcg cttttctaga gaaaaatttt gttttagaat ctgaaaatat
     6121 tttttcgaaa caacaattgg atgctgctat aaacaccatt cagtcaaact tcgatagggt
     6181 agacggtggc tttggaagtg cacctaaatt ccccgcaacc atgactctgc gatttttgtt
     6241 gcgatacgca tggttcacaa ataataaacc aattctcgat catgtaattc tttcactcaa
     6301 taaaatgcac cagggcggaa tcttcgatca gattggtggc ggattcgcaa gatatgctac
     6361 cgataataaa tggatgatcc cgcactttga aaaaatgctt tacgataatg cactcctatg
     6421 cagtctttat gccgaagcgt atagggctac cggagataat aattatttgc aggtagtgaa
     6481 agacattatt caatatatcg atcgagaatg tagcgatgaa cttggcggtc tttatgctgc
     6541 atatgatgcc gatagcgaag gagttgaagg actatattat acattcactt accaagacct
     6601 tgaaaatgct ttgggtgatg atgccgtatg ggcagcgaaa gtgtttaatg ctacctcagc
     6661 ggggaattgg gaacatacga atatacttca ccgcgatgcc tcagatgaaa caactgcgcg
     6721 acaattggat atgacgcacg aagcattctc ggcaaagctt aagcttgtaa aaggtaaatt
     6781 attgcaatat cgtgaaaagc gaatcaagcc cgcacttgac aataaaattc ttcttagctg
     6841 gaatgcattg atgtgtagcg catttgtaga cgcatataag gcttccggcg aagagcaatt
     6901 cagacaaaaa gcgatacaac ttgttcaaca tttaacaagt gctttcaaaa gaaagaatac
     6961 tgaaaatgca tactggcatg tcatcaccaa caacattgca aaacaaacag cttttcttga
     7021 cgattatgct acattaattc aagcattatt tgatgtgttt gaaattaccg gcgatgaagc
     7081 atggtttaaa caagctgatg gctgtatgga atatgtgatt gcaaatttta gtgccaccga
     7141 tggaatgttt ttcttcacgg agaaatcgca aacagatttg ccttcgagaa atgctgagta
     7201 ttatgataat gctacaccat ccggtaatag tattatgctt tcaaattttc agagaagatc
     7261 aattatgtta ggaaatacta aagatgtaga aagggtccgg acaatgctgg gtgctatgaa
     7321 aaattcatta ataaaatacc ctacttcatt tggtaattgg tggttgtcgg cattgaaaca
     7381 gatttaccct tcagttgaaa ttgcagtaac gggacccgac catcaagtga tgcgcgatag
     7441 aatttttaca cattatcatc cgcatatttt ggtacagaac ggcaacaatg cagtatccga
     7501 attccccctg ctccaacaca gagcaagtga tgtcgagacc agaatatttg tttgtcacaa
     7561 tttcacctgt aaaaagccgg tcaccacatt gcaagatttt gtttctgagg tgcagacact
     7621 tggataagcg tacagtgtgt agtaccttta tcagcgcata cggaaatatc atattttacg
     7681 ttatagttgc aatgaagcag atctacgcaa gaattacatg tggcttggtt ttcagtgttg
     7741 ctgtgtttaa cgcacaggcg cagttgatca gctacggtca gcaagatccg caattcaccc
     7801 aatacatgct taatacctcc ggattcaacc cggctgccat tggtgtagag gacgcttggg
     7861 tttcaaccct tggtgtgcgt agtcagtggg taagtttacc cggagcgccc gtgtcacaat
     7921 ctttaacttc tcacgcacca atttataaaa tcaatagtgc ggttggcgtg acagtgatta
     7981 atgacattat gggacagcaa cgtacaacgg gggcatcact ggattatgcg tatcacaagc
     8041 gttttgggcc gtcgacattg agtattgggg ctagtggcgg tatttttcag gagacaataa
     8101 atggtgcaaa attggtaacg ccaacaggca attatgaggg tggtgtgaac cataatgacc
     8161 ctaatttgcc tgtcacaact atttcaaaaa tgatacctga tgccgccttc ggtatgtatt
     8221 tctttggccg gaatttgact gcggggatat cagcaaacca tttactgctg ccgattacag
     8281 cgggaaacga tgcaaattcg ggcacagtac aatataatcc caacggatac gtttatgtcg
     8341 catatggttt tgatgcgggt aaccggttcc gtattatgcc aaatgttctt tataaaaccg
     8401 atctcaatga aggtgttgtc gacctcaaca cccttgtcac atacaacaac aacatccaag
     8461 caggaatatc ctgcagagcc aatacagcac tggctttcga tgcggttgca ctgatggcgg
     8521 ggatgagggt tgctgatcgc tggttactca gctacagcta tgatgtcacc ttgtcggctt
     8581 tgaacacagt tagttcaggg tcgcatgaga tagtagtaag atacagcatt ccggtggcca
     8641 gaccgcgtgc agggaagatg atcaacaacc ccagatttct gtatcattaa caaccaaaaa
     8701 tttgtgattt ggtaaaaagt ggctaatttc ggcctacttt tacccaatct gtgtgtataa
     8761 acagtggtaa cacaactttg tattacgtta aaaatgctat ttttacacgg aataatcagc
     8821 acaaattgat ctcaagtatg aaaagaaatc tgcaattgtt cacccttgca ttcgtggcca
     8881 tcatcgttgg ctcaggctgt agtcattcga gttgggatga ttaccgcggt caactcctcg
     8941 gcatgcagga ccgtccggaa tgggagcccg ttaatccata cgggatggtg tacattccgt
     9001 cgggaaccct tcacatcggt ccgagcgatc aggatgtgaa caactcacta tgggcacgag
     9061 cgaagtccat ctctatccag ggcttctaca tggatgatgc cgaaatcact aacaacgagt
     9121 accgtcagtt cgtttactgg gtgcgcgact ctatcatgca cacactcctc gaccactatg
     9181 tggaagaaga agaaggcatg gaatcagagg tgcctctgat cgactggagt caggaaatcg
     9241 actgggacga tgaggaaaca cgctcacgcc tggaagaact ttacctgcct gccagcgaaa
     9301 cttactatgg tattaaggca atcgacactc gtcagttgca gtataactat cgttatgtga
     9361 agtggaaaga ggctgcaagc aaatcaaacc gcgataaaaa catcgctgag ttcattgttg
     9421 aagagccgat agaaatctat ccggatacat tggtatgggt gcatgattac acctattcat
     9481 ataatgagcc gatgactcgt cagtatttct ctcaccctgc attcgatgat tatcctgtag
     9541 ttggggtgtc atggaaacaa gcaggtgcct tcgctacatg gcgttcagat ttctggaacg
     9601 actggcgcag agcacatgaa gaagtgcaag tcgacgattt ccgtttgcct acagaacacg
     9661 aatgggaata cgctgcccgc ggtggtcgcg atatggcacc atacccttgg ggtggtcctt
     9721 atgtgcgcaa taccaaaggt tgctacctcg ccaactttaa acccggtcgc ggtaactatc
     9781 ctgaagacgg tggctactat accgtgaaag tatattcata tgctccgaac gatttcggtt
     9841 tatataatat ggcaggtaac gtttctgaat ggacaagcag cgccttccag gaaaatactt
     9901 attcattcct ccacgatgag aaccctgact atcgttatga tgcagaggac agcgatcctc
     9961 ctgctttgaa acgcaaggta ctccgcggtg gctcatggaa agatatcagc tattatatac
    10021 agaccggctc tcgtcactgg gaattccagg atactgcaaa atgttatatc ggcttccgtt
    10081 gctgtatgac attcctcggt cgttcaatga gtgattttta atctgattac ataattcccg
    10141 ataagcctaa cttttttatt tagtacttca atcatttctt aaaaccgtta aaaaacaaaa
    10201 gcaaaatgtc agcatttttt gaatccgatc gttttaaacg aatcaaaaac ctgatcatcg
    10261 gtattggagc atccgtagta ttggtgggtg cattattcaa gatccttcac tggccgggtg
    10321 ccgaccagat gttgatggtt ggtatgctta ccgaggcatt cctcttcctc atgctgggta
    10381 ttatcccacc acacaaagac tattattggg aaaaaatcta tccggacctg gatatcgctc
    10441 ctgaagttga agaagctaaa tccggaaaga aagtacacaa aatgactgag ccgaaagaaa
    10501 gcgtaactcg tcagctcgac aaaatgttgg aagacagcaa agttgagccg gtgatgatcg
    10561 aacgcctcgg tgaaaacctg aaaaaactcg gtgaaaacat cgagaaactt tcagatgttc
    10621 aggatgctgc cgtaaatacg aatgagtatt ctaacaaaat taaagatgct tcaacagctg
    10681 ttgctgaaat gcgcgatgct tatgcaaatg cagctcgtgc tatggaacag attactgcat
    10741 cttcagttga tacatctaaa tatcatgagc aagtgcaaat ggttactaaa aaccttagcc
    10801 agctcaatgc tatttatgaa ctcgaacttc aggatacaaa taaccacctg aaagcaatga
    10861 acaaattcta tggcagcctt aactctgcaa tggataacct gcaggaatca cttaccgata
    10921 cttctaaata tcgtgaagag atcgctcagc tttctaataa cctcgcagcg ctcaaccgcg
    10981 tgtatggtaa tatgttaagc gcaatgagca tgggtgctgc ccgttcataa aaacaattaa
    11041 tgatcaactt ttacttaact aataaaattt aataaacaat gtcaatacct aaggaaccac
    11101 gccagctcat gatcaacctc atgtacctgg tgcttacagc ccttttggca ttgaacgttt
    11161 ctgcggaaat tctgaatgct tttaatactg tgaacaacgg tattagctcc agtaacgaac
    11221 tcctcaccag caaaaatgag ttgatgatga ctatgatatc taaggctgct gccaaagatg
    11281 ctcgtcctga aactcaagaa atccttcgcc aggcaactga agcgaagaaa atgagcgatg
    11341 agtttgtaaa gtatgtagaa ggtttgaaag aagaggttaa agctcgcgcc ggcggtccaa
    11401 atcctgatca tcctgaaaaa ttaaaaactg aaggtgaaat ggagaaaaca agccgcctgt
    11461 tcctcaatga aggaaaaggt aaagagcttg aggctaaaat caatgacgca cgtgagaaat
    11521 tcttgaatgt gcttggtaaa gaaagtggta tcgatgtgcc actgcgtatc gtagaagtac
    11581 ctgaagggga acatgctacg aactgggaag aatataattt cgatcgcgtt cctgctatcg
    11641 ctgttatgac catccttacc aagtttcaac aagatgcgaa atcatctgaa tcaaccatcc
    11701 tcgactacct ccgccgtaaa atcaatgcag aagacattca agttgataaa atggctgcca
    11761 tggttgtagc tcctagctct tatgttcgca agggctccga atatactgct gacattttta
    11821 tcggagcaac ttctaagaac attaaaccgg aagtgtacgt gggaactttc acaagtgcag
    11881 tgaaaaaaga cccagccgac cctacaggaa cagcgtttct tgaagtggaa ggaaaagaca
    11941 ttccactgaa tggcgctcgt ccaatcgata tggttaatgg tatcggtaaa atcaaagaag
    12001 gcgctaacgg acaacgcaat ttccaaggcg ttatcaagat tcctcacccg tctaaagaag
    12061 gtgaattccg cttctatcct tttgaagccg gttacgaaac tttcgaagtg ggtaaggccg
    12121 ttatctctcc tacagcaatg aacgtattat atattggtgt agataatcct atcaaaattt
    12181 ccgtaccggg ttatgcttct gaaaaagtaa gcgcatcagg ttgcggtgtt accaaagtga
    12241 aaggcgaaga atatgttgct cgcccttcaa aagttggtga agaagaaatc gtagtaagcg
    12301 ttcaaggaaa caaaggcgtt gaaaaatctt ctgagaaatt cagaattcgc cgtgtgcctg
    12361 atccatatgt atacatcctg aatactaaag gtggtccaat gaagatcggt gaatttaaag
    12421 ccgctgatgg tatcattgtg aaaaaccctg acttcgtatt ccaggtgcct tatacagtta
    12481 ccggtttcga aatgctctac gctccgaaag gtagtggcgg tgtttctgat atgtctaatt
    12541 cgtctaaatt ctctagcaca atgaaagaca ttcagaaaaa atgcaaaccc ggcgatacta
    12601 tcgtgttgaa cgatgttgcc gtgaaaatgc ctgacggtcg tacagtaaaa attagcgcct
    12661 catttaaatt gattggcggt taatcttaag ttgaataaaa ggtatgaata gaacattgtt
    12721 aattctcggt ttggtactgg tacttggtac tgccgtacat gctcagccaa ccagccctgc
    12781 aaagctggat ggcatctata agcgtgaact gctcggtaca cgtgagatca ttccttacga
    12841 tgatatccgc gaagctgata tctattggga gaaaaggatc tggcgtaata tcgacttccg
    12901 tgaaaagatc aatttgccat ttacctggcc cgtagatcct ttcgtacaag ttgtgtatga
    12961 tgcggttatg agtggtgaac tcactgcgta tagcccattg tatgacgact ttcgcgaagg
    13021 aactgccatc cctacggatg aactcaaagc gcgcttcaat aaagtggata ccacatggat
    13081 cctcgatgaa acaacttatg aggaggtgcc ggtaatcgta gtgaacgagt tcgattataa
    13141 gaccgttcag aaaatacaga ttaaagaaga ctgggtattc gatgaagaaa cttccacaat
    13201 ggtggtacgc atcatcggac tctccatgat tcgcgatcgt attgacccta caacaggtga
    13261 agtgatcact accgaaacaa tgttctggtt atactatccg gatctgcgta acgtgctgat
    13321 acgtcatgaa gtattta
//
LOCUS       DAOQHY010000091        13192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2293_length_13192_cov_5.296034, whole genome shotgun sequence.
ACCESSION   DAOQHY010000091 DAOQHY010000000
VERSION     DAOQHY010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13192
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2293_length_13192_cov_5.296034"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2492)
                     /locus_tag="PLL28_08675"
     CDS             complement(<1..2492)
                     /locus_tag="PLL28_08675"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69435.1"
                     /translation="MKPVLFLLSLTASALLVQHVSSKKSAAPIRADQSSTADEWLYQQ
                     KAFPYDRPDRVAQRQAVADRLQQRNQMLMQKTEDVDDWHFIGPITIGGRILDIVCPVG
                     DDNTIYVGTASGGVLKSTNGGDSWANIFDAQSTLSIGDLAIDPNESNVIYVGTGEPGN
                     GVGSVTYDGNGIYRSNDAGITWTNIGLTNGGNTGRIAINPQNTDIIFTAMLGDLFENT
                     PDRGIYRTEDGGTTWEKVLYVNDSTGGIDVCINPENPDIVYAATWERVRRYNRRDYAG
                     QGSRVYRSEDGGDTWEMLTNGLPAGTQELSKISIAVAPSNPDYVYATVVGSNDKLKDI
                     YKSIDGGDTWIALNCTAEVTTTSQDNWFGGVRINPNDENEIYWVGFYVSRSTDGGLNW
                     TTITEDAHVDCHALYISPTDDNFRIMGSDGGLFFTYNDFISYTFEQNIPVTQLYTLDV
                     YPGDTSRLIGGAQDNGSFTRDAGGDWAFVNWGDGVSTRFVPVDESSFYGNYQYGGYYG
                     IVGGNYMDLFGFDFDERFNWRSPMELNPLNPQTIYFGGNTIYRTTNYGESVSQISGDL
                     TDGSQGIGLTFGSIFTIHNAPADTNYIYVGTDDANVWRSDDYGDTWTSITDGLPYRYC
                     MSIETHPDNAQQAFVAFSGFRWAEDIAHIYMSENAGDTWTPIDGDLPDIPVNDIQVFT
                     IGDTTGLAIATDVGCYYSYDLGEHWFALGAAMPIVSVYEIYYDAETYTLFAGTYGRGA
                     WKIEMPRAEPIVQSATQYTDVTLTLWPNPASDKANLHFNRKVADVDISILTMSGEVVF
                     QTTYSGVRDVSIPVADLPQGTYMVVAEWDGKR"
     gene            complement(2622..3302)
                     /locus_tag="PLL28_08680"
     CDS             complement(2622..3302)
                     /locus_tag="PLL28_08680"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNF69436.1"
                     /translation="MKRLLIICLGILSAFPMMAQNEGEINGKVIDGETGNSVPYAHCI
                     LLSSGAKVMEVEADDAGLFYFKPLKPGTYDLNVLFVGYDTAKLESIELASKGMVVLNV
                     EMSVGTILPPVYVGAPLVDKQVPEVVKTYKAKDIGHMAVDDAQDIIRLAPAVYEDEKT
                     GGMSIGGSREDATLYVVDGVKVIGPLYVPINSIAEVRIITGGIPANYGDVTGGVVEIT
                     TKGYSGVY"
     gene            complement(3842..5617)
                     /gene="sppA"
                     /locus_tag="PLL28_08685"
     CDS             complement(3842..5617)
                     /gene="sppA"
                     /locus_tag="PLL28_08685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNF69437.1"
                     /translation="MKPFFNSFFASLLALLVFTILGFFIIAGIAGAISQSFEKEKTSV
                     EGNSVLKLNLTYSIVEQTQSGMPGGLALLGLEADRGLGLNDILASIKHAETDENIKGI
                     YVQMGISPNGYATLQEIRDALADFKMESGKFVIAYGEVIGQNAYYLGSVADKVYMNPA
                     GAIDFKGLSAQLTFYKGALDKLGVTTQVFYDGKFKSATEPYRMDKMSPENRLQLEVFL
                     NGLFDANVSEIAADRKMQPMQCREIADSLLAWYPTEAVRLGLLDGLKYYDEVLDEMKS
                     RMGIANDQELKIVAMSDYRNSFTIPKVSDSKGTVAIVYADGTIIDGKAGNGFLGSADF
                     VEMMQQVREDENVKSVVIRVNSPGGSAVASDVMWREIEITKKKKPVVVSMGDYAASGG
                     YMISCNANKIYAQPNTLTGSIGVFLIVPEISDLMEDKLGITFDTANTGMYSDFPSITR
                     TFMPREQQILQSGVDSTYLHFKQMVAAGRNLSVEQVEEIAQGRIWIGEEAKKIGLVDE
                     IGGIDEAIAAAAKLGEIETYDIKEFPIQKESILDQVLVNLTEETKMQMQAEELGFMYP
                     HYRNLKELLERPVMQARMPYILEIK"
     gene            5714..6220
                     /gene="folK"
                     /locus_tag="PLL28_08690"
     CDS             5714..6220
                     /gene="folK"
                     /locus_tag="PLL28_08690"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNF69438.1"
                     /translation="MMTEHTAYLLLGTNLGNRSENLSKATAAIRMFLGRVESQSHIYE
                     TEPWGKPHQPNFYNQAIKVVTPCSPLETLHLMKQVEFLLGRDNSEKWAPRVIDIDILF
                     FDELAIESPVLTVPHAHIAQRRFTLDPMSEIAPDLVHPVLNKTIKELADACTDTLAVQ
                     MVDVVYQF"
     gene            6285..6917
                     /locus_tag="PLL28_08695"
     CDS             6285..6917
                     /locus_tag="PLL28_08695"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNF69439.1"
                     /translation="MAHQIITVEGNIGSGKTSFATKLAKRFEAKLVLETFADNPFLQK
                     FFIKQQDYALGMEMFFLAERYEQLNNEMMDAELFQNRYVIDYLFNKSLLYARVNLDDA
                     EFALFQKIFNILNPKLRKSDVIVYLHSDLDRLVANIYKRGREFELTVKKDYLKKIETI
                     YLEYFRQAPDQKVLILDVTKADFVHNEADFDTMYDIVMQEHAIGVQRFEI"
     gene            6981..7397
                     /locus_tag="PLL28_08700"
     CDS             6981..7397
                     /locus_tag="PLL28_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69440.1"
                     /translation="MKSTKIAIIALLAYVGFGFTTIPGGEQQVKVSVGWEFKNLVEGF
                     DHDCKTELYIDGTLVATSSVTKESKANSVSATTSRGKHEIKVINYAFYEGNWEEHTVA
                     NNYSIDATYTDNINCKKKNTKIHLIFDIDKGTSVKK"
     gene            7529..9211
                     /locus_tag="PLL28_08705"
     CDS             7529..9211
                     /locus_tag="PLL28_08705"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNF69441.1"
                     /translation="MSQAQIPSLYLENKTPTYDETIAYFKHIDSMCDYMQMRSYGTTD
                     AGFPLDVLVIDGDKQFTPEAARAAGKAILLINNGIHPGEPDGIDACIQLITQYAGAGD
                     LKSALEGLVICIIPVYNIDGALNRNSTTRVNQEGPESYGFRGNGRNYDLNRDFVKCDT
                     RNSKSFAEIFHLWDPDIFVDTHVSNGADYQYVMTLIPTQHNKLTPPLGALLKNDMLPS
                     LYKAMEGKGFPMCPYVNEVDRIPDNGIAGFLDHGRYSTGFTALFNTIGFMPETHMLKP
                     YPQRVASTLAFIEVVIDYIQENRSAIIEARAVAKQTVIQADTFNLQWKLDTEQQESFM
                     FNGYAAKFKKSDVSGLDRLWYDRNEPFTKPIPFFDTYTPTLSIVAPEAYIVPQAWLPV
                     IERLQWNGVRMERLEKDTAISVESYYITGFDSVDRPWEGHYFHSNVKVRKVTETLNFM
                     KGDYIIPLNQESNLYIVQVLEPQAPDAFFAWGMMDAVLQQKEWFSDYVFEDLAAEILQ
                     QDPQLRNMLNEAMIADPSLKNDAEGQLAFVFEHSKYKEPTHNRYPIARIVKK"
     gene            9264..10073
                     /gene="cdaA"
                     /locus_tag="PLL28_08710"
     CDS             9264..10073
                     /gene="cdaA"
                     /locus_tag="PLL28_08710"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNF69442.1"
                     /translation="MQAAFKIGFLEITPVDVVDVLLVAFILFQLYRLVRGSLAINIFI
                     GLLMVYVLSLIAAALELTLMSDILGAFIDVGVIAVIIVFQPEIRRFLLYVGRSSDFRK
                     VRFWKALSLRKIRTGEYNQKEVNALTEAIMALSESKTGALIVIPQTSQLGFYQNTGIH
                     LNALITKDLIESIFYKNSPLHDGAMIISEHRIAAVKCILPLSDNPDLPGKLGMRHRSG
                     VGITEQSDATCIIISEENGEISYAREGKLYQQMQKEEIRKLLSRALFHSVN"
     gene            complement(10053..10616)
                     /locus_tag="PLL28_08715"
     CDS             complement(10053..10616)
                     /locus_tag="PLL28_08715"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF69443.1"
                     /translation="MNRAYGLMLMIVACTFASCDQRGKEIKEIDAMAVKMDSVAKANP
                     VFTVAHKVEAEALITEYLEFVRQYPKDTLCAQYLMSAARLYEKMPAVKEELQLLDRLI
                     EEYPASPLVPQALITAANSCELVLRDYNRAIAYYRTLQEKYPDSPYSKNIDLQIEFIG
                     DPDGLFTAIMERAGKPVDTLESVNAVK"
     gene            10643..11959
                     /locus_tag="PLL28_08720"
     CDS             10643..11959
                     /locus_tag="PLL28_08720"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HNF69444.1"
                     /translation="MYYNQTLVKLFALVVTAIFASSCNRIVVRYAGESDLIQASLPAP
                     GKVCKESENYIIDTNHLNATPIRYVRVNFHIMQESDGSGSWNEAEGRKFVHDIVEQSN
                     NRWNDNQPMTLPLGNSTPDLPIPIRIVLQRDPITGEEGIYFHRDDTLCYWNRSATKGP
                     NSLSDRTVISKYQVGIDSIINIFLIEHDPDSIGSPTYGEPKMSGISFINSIKLFSMYY
                     QGHIVKYTNAGVPFTHDAFYFSKLLNHELGHCFNLNHTWNWDDSCDDTPKNSGCWSVT
                     GVPPCDTPNSNNMMDYNISQQAVTPCQIGRMEYNFTKETGGARQFAIPYWCEYHAFER
                     VVVYRNENLEWNGAKDLWGDIEIRDGATLTIRCTVSLPEQAKVIIKPGGTLIIDGGTL
                     TNRCGDKFEGVEIWENKKTDQIGKVMISNGGKLENMVNYTTITTTE"
     gene            11969..12544
                     /locus_tag="PLL28_08725"
     CDS             11969..12544
                     /locus_tag="PLL28_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69445.1"
                     /translation="MTFTSEQYRSFKLLSPAGCIIALLGFFLTFTEINCNGTTLDTIT
                     GVELATGYSPDIDLTESNDEPETKQEKYDPNVFALNAWIAAMLGLALFLIPKMRDQHA
                     LHLIIALIGFGCMIGLMFNLKSALNDAKSSGGVVNVDLPITFDMQPGYWIVTASFFLT
                     LVADIVLWMHQRKVIADLHASRQPDNESNLS"
     gene            complement(12605..>13192)
                     /locus_tag="PLL28_08730"
     CDS             complement(12605..>13192)
                     /locus_tag="PLL28_08730"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNF69446.1"
                     /translation="NTYLNADYKTQLEDKYKQMEDLHGWGSFDVNLHVWRSRVNMAKG
                     FIDFNYGMSLEWHHFRYDQNFSILPNMDSLTLATETLDYDKNKFNTTHLAIPLLLGFE
                     TKPWDPENSFRMQFGYSPGLMLRGKTKHKLDGKKDIEFDDFNLAQFRHELNYVIGYGD
                     FNVYASYDLNSMFAEGEGPELHPFSVGLIIRRGFE"
BASE COUNT         3894 a         2958 c         2608 g         3732 t
ORIGIN      
        1 atgcgcttcc catcccattc tgcaaccacc atataagtgc cctgaggcag atcagctacc
       61 ggaatggaaa catctctgac tccggaatac gtcgtttgaa acaccacctc tccactcatc
      121 gtcaaaatgc tgatatccac atccgcaacc tttctgttga agtgcagatt cgccttatct
      181 gacgcaggat ttggccaaag tgtgagtgtt acatccgtat attgtgttgc cgactgcacg
      241 ataggctccg cgcgaggcat ttcaattttc catgcgccac ggccataggt gccggcaaaa
      301 agtgtatatg tttctgcatc ataatatatc tcataaactg aaacaatcgg cattgcagcg
      361 ccaagtgcaa accaatgctc accgaggtca taggaataat agcatccaac atcagtagca
      421 attgctaagc ctgtagtatc tccaatggtg aaaacttgaa tatcattaac cggaatatcc
      481 ggaagatcgc catcaatagg tgtccaggta tcacctgcat tttccgacat atagatatgt
      541 gcgatatctt ctgcccatcg aaaaccactg aaagccacaa atgcctgctg cgcattgtcg
      601 ggatgcgtct ctatactcat gcaatagcga tatggcagtc cgtctgtaat cgatgtccag
      661 gtatcgccat aatcatcact tcgccaaaca tttgcatcat ctgttcctac gtaaatataa
      721 ttagtgtcgg caggcgcatt gtgaatggta aatatcgacc cgaatgtcag accgattccc
      781 tgactgccat ctgttagatc accacttatc tgagaaacag attccccgta gttagttgtg
      841 cgatatattg tattcccgcc aaaatatatt gtttgcggat taagcggatt taattccatc
      901 ggactacgcc aattaaatcg ctcatcaaaa tcaaatccaa ataaatccat ataattcccg
      961 ccaacaatac cataatatcc gccgtattga taattgccat agaaggaact ttcatcaacc
     1021 ggaacaaatc gcgtgctcac accatcaccc caattcacaa aagcccaatc tcctcctgca
     1081 tcgcgagtga aggagccatt atcttgtgca ccgccaatca aacgggatgt gtctccggga
     1141 tacacgtcga gcgtgtataa ttgtgtaaca ggaatattct gctcgaatgt gtacgaaata
     1201 aagtcattat aagtaaaaaa taatccgccg tcacttccca ttattctgaa attatcatca
     1261 gttggtgaaa tgtataatgc atgacaatca acgtgtgcat cttcagttat tgttgtccag
     1321 tttaatccac cgtcagtgct tcgcgataca taaaatccta cccaataaat ttcattttca
     1381 tcattcggat ttatgcgcac tccaccaaac cagttatcct gcgatgtggt agtcacctcc
     1441 gcagtacagt ttaatgctat ccacgtatca ccaccgtcta ttgatttata aatgtctttc
     1501 aatttatcat tgcttcctac aactgttgca tatacataat caggattaga aggtgcaacg
     1561 gcaattgata ttttactcaa ttcttgcgtg ccggcaggta atccgtttgt aagcatttcc
     1621 caagtatcac caccatcttc actcctatac acgcgactac cctgtcctgc ataatcacgt
     1681 ctgttatatc tgcgcacacg ttcccaagtt gctgcgtaaa cgatatcagg attttcagga
     1741 ttgatacata catctattcc acctgtagag tcgttgacat acaatacttt ttcccatgta
     1801 gtgccaccat cttcagtgcg ataaattccg cggtcgggag tattttcaaa caaatcgccc
     1861 aacatcgctg taaaaatgat atccgtattt tgtgggttga tggcaattct tcctgtatta
     1921 ccaccatttg tcaacccgat atttgtccac gtgattccgg catcgttgga acgataaata
     1981 ccattgccgt cataagtcac agaaccaacg ccatttccgg gttcgcctgt gcctacataa
     2041 atcacattac tttcatttgg atcgatagcc agatcgccaa tggataatgt tgattgcgca
     2101 tcaaaaatat tggcccagga atctcctcca ttcgtagatt tcaatacacc tcccgaggca
     2161 gttccaacat aaatggtatt atcatcacca accggacaaa caatatctag aattctgcca
     2221 ccgatagtaa ttggtccgat aaaatgccaa tcgtcgacat cttctgtttt ctgcatcaac
     2281 atctgattgc gttgttgcaa gcgatctgcc acggcctgac gctgggccac acgatcaggt
     2341 ctgtcgtacg gaaaggcctt ttgttgatat agccattcat cggctgttga ggattgatcc
     2401 gcgcgaatgg gtgcagcaga ttttttactg ctgacatgtt gaacaagcaa agccgatgct
     2461 gtcaacgata gtaggaataa tactggcttc atataaaaga atgtataaat atacgatact
     2521 ctccctgcag gcacaaaaaa accgccttct gagcatggaa ggcggtttca agcaaacaat
     2581 acagctgctt gcgcaggact caagtattaa aatttatggt atcagtaaac tccggaatat
     2641 ccttttgtgg tgatctcaac aaccccaccg gttacatcac cgtaattggc cggaatacca
     2701 cccgtaataa tgcgaacttc agcaatcgaa ttaattggta catacaaggg gccaatcact
     2761 ttcacaccat ccacaacata taatgttgca tcctctctcg aaccaccaat cgacatccca
     2821 cctgtttttt catcttcata aactgccgga gccagacgaa taatatcttg cgcatcgtca
     2881 actgccatgt gaccaatatc ttttgcttta taggtcttaa ctacttcagg aacctgttta
     2941 tcaactagcg gtgcaccaac ataaacagga ggaagtatag tgccaaccga catttctaca
     3001 ttcaacacta ccataccttt cgacgccaac tcgatagatt caagttttgc tgtgtcgtaa
     3061 cccacaaaca atacatttaa atcgtatgtg ccgggtttta gaggtttgaa ataaaataaa
     3121 ccggcgtcgt ccgcttcaac ttccatcacc tttgcaccgc ttgacaataa aatgcaatgt
     3181 gcataaggta ctgaattgcc ggtttcacca tcaataactt ttccgttaat ctcgccttca
     3241 ttctgtgcca tcatggggaa agcagaaaga attcctaaac aaataataag tagtcgcttc
     3301 ataatgtgga tttttatcaa aagtatctta tgggttttgt tcaattgtca cggaaatgac
     3361 agatgacaga aataggagaa aggagagagg agagaggaga gaggagagag gagagaggag
     3421 agaggagaga ggagaaatgt gggaatcccg ctgaggcggg atgtcgctgt gctccatgtg
     3481 ggaatgccag cctcgccgca ggcgggtgag aatgtgggaa tcaatagcgt ttgcaactcc
     3541 tgtaaaattg caattttcat aacctttaat ttaaacctta agaataattc atcatcaaat
     3601 acaattaatc ccccctctct gcgacgccag gagtggagag ggggtcaggg ggtgaggtgg
     3661 ggttcgcgca aagtcgcaaa gttggcactc acgattcacg agtaacgagt cacgattcac
     3721 cactcaccca ctgacccact cacccactca cctactcaca acgattgctg aaaataaaat
     3781 cggctccgaa gcattgaaat tcggagccga ttttgtgagg tgaattaatt tttttgatta
     3841 cttatttaat ctccagaata taaggcattc ttgcttgcat cacggggcgt tctaataatt
     3901 ctttgagatt gcgataatgc ggatacataa accctaattc ttctgcctgc atttgcattt
     3961 ttgtttcttc cgtcaggttt accagtactt gatcgagaat gctctctttt tgaatcggaa
     4021 attctttgat atcgtaagtt tcaatttcac ccaatttagc tgctgctgca atggcttcgt
     4081 cgataccgcc gatctcatcc accaatccga tttttttagc ctcctctcct atccatattc
     4141 tcccttgtgc aatttcttcc acctgttcta ccgacaaatt tcttcctgct gcaaccattt
     4201 gtttaaaatg cagataagta ctatctacac ccgactgtaa aatttgttgc tcacgcggca
     4261 taaatgttct ggtgatagaa gggaaatctg aatacatacc tgtatttgcc gtatcaaatg
     4321 tgataccaag tttatcttcc attaaatccg aaatttcagg tacaattagg aataccccaa
     4381 tgcttccggt caaggtattt ggttgagcat aaattttgtt ggcattgcat gaaatcatgt
     4441 acccgccact ggcagcataa tcgcccatag aaacaacaac aggtttcttt tttttggtga
     4501 tttcaatttc acgccacatc acatcactcg caacggcaga accgccgggt gaattaacgc
     4561 ggatcaccac agattttacg ttttcatcct ctcgcacttg ttgcatcatt tccacaaaat
     4621 cagcagaacc taaaaatcca tttcctgcct taccatctat gatggtacca tcagcataaa
     4681 caatagcaac agttcccttg ctatcagata cttttggaat agtaaatgaa ttgcgataat
     4741 cgctcattgc tacaattttc aattcttgat cattggcaat gcccatgcgc gatttcattt
     4801 catccaacac ttcatcataa tatttcagtc catcgagcaa acctaatcgc acagcctcgg
     4861 ttggatacca tgctaaaagg ctgtcggcaa tttctctgca ttgcatcggt tgcatttttc
     4921 tatctgcggc gatctcactc acattggcat cgaaaagtcc gttcaaaaac acttccagtt
     4981 gcaagcgatt ttccggactc attttatcca tgcgataagg ctcagtggca ctcttaaatt
     5041 tgccatcata aaatacttgc gtagtcacac ctaatttatc caacgctcct ttataaaatg
     5101 tgagttgcgc actcaatcct ttaaaatcta tagctcctgc cggattcata taaactttat
     5161 cggcaacgct tccaagatag taagcattct gccctattac ctcaccgtaa gcaatgacga
     5221 atttcccact ctccatttta aaatctgcca gcgcatcacg aatttcctgc aatgtggcat
     5281 atccatttgg actaataccc atttgcacat aaatcccttt gatgttttca tcagtctcag
     5341 cgtgcttgat ggaagccaga atatcattca atcccaaccc gcgatcagct tcaagtccga
     5401 gcaatgccaa tccgcccggc attccacttt gcgtttgttc tacaatggaa tatgttaaat
     5461 tcagttttaa aacagaattt ccctccacag aagtcttttc cttctcaaag ctctgtgaga
     5521 tagcaccggc aatgccagct attataaaaa atcctaatat ggtaaatacc agaagggcca
     5581 gaagactggc gaaaaaggaa ttaaaaaacg gtttcataaa ttttcttact ctttattacc
     5641 tacttattga tgaatgcact aattttcgca ggattacgtt attcccctaa aggaaataac
     5701 tcctcgacag ccaatgatga cggaacacac cgcctattta ttgttaggga caaatttagg
     5761 taaccggtcg gagaatctct ctaaggccac ggcagccatt cgtatgttcc ttggacgtgt
     5821 agaaagccag tcgcatatct atgagactga gccttgggga aagccacatc aacccaattt
     5881 ctacaatcag gcaatcaaag ttgtaacacc ttgctcaccg cttgaaacgc ttcacctcat
     5941 gaaacaggtt gagttcctgc tgggacgcga taattcggag aaatgggctc caagggttat
     6001 tgatattgat atcctgtttt ttgatgaact agccatcgaa tcacctgtgc ttacagttcc
     6061 acatgctcac attgcccagc gtaggttcac actcgatcca atgagtgaga ttgctcccga
     6121 tttggtgcat cctgtgttaa ataaaaccat caaagagctt gcagatgcct gcacagacac
     6181 cctcgccgtg caaatggttg atgtcgttta tcagttctga caggtgaact caacattttc
     6241 aattttattc ttcgttttcc tgtatttttg acgccctttc aggtatggcg catcaaatca
     6301 ttacagtaga aggtaatatc ggttccggca aaacatcgtt tgccaccaaa ctggctaaac
     6361 gcttcgaagc gaagctggta ctcgaaacct ttgccgacaa ccctttcctg caaaaattct
     6421 ttatcaaaca acaagactat gccttaggca tggaaatgtt cttccttgct gaaaggtatg
     6481 aacagctcaa taatgaaatg atggatgccg agctgtttca aaatcgttat gtaatagact
     6541 atctgttcaa taaatcactg ctctacgcgc gtgttaacct tgatgatgcc gaatttgcgc
     6601 tgtttcagaa gatcttcaat atcctcaacc ccaaactgcg caaaagcgat gtgattgttt
     6661 acctgcactc tgacctcgac aggcttgttg ccaatattta taaacgcggc cgcgaatttg
     6721 agctaacggt aaagaaagac tatctcaaga aaattgaaac gatctacctc gaatacttcc
     6781 gccaggcacc cgaccagaaa gtattgattt tagacgtcac caaggccgat ttcgtgcaca
     6841 atgaagccga ttttgatacg atgtacgaca tcgtaatgca agaacatgca attggcgtgc
     6901 aacgatttga aatctaatat agtctagtga ttgtcagata tatgtcagaa atatatatat
     6961 ttgtgacaca aattcaagtt atgaaaagca ctaaaattgc aattattgcc ctcctggcct
     7021 atgtcggatt cggattcacc acaattcccg gtggtgaaca gcaagtaaaa gtttctgttg
     7081 gatgggaatt caaaaacctg gttgaaggat tcgaccacga ttgcaaaact gaattgtata
     7141 tcgatggcac actggttgca acttcaagcg taacaaagga aagcaaagcc aattcagtct
     7201 ctgctacaac tagccgcgga aaacacgaaa tcaaagtgat caattacgcc ttttatgaag
     7261 gcaactggga agagcatact gtagccaaca attatagtat cgacgctacc tatacagata
     7321 atatcaattg taaaaagaaa aacaccaaga tccatctcat ttttgatatt gataagggca
     7381 cttcggtgaa aaaataattc cattcaaaat gcacacaatg aaacctttcg gcacaactga
     7441 aaggtttttt tatttttatc ccttccatca ggcactccag gctatgcgaa ctattatttc
     7501 cgttttatcc atacttactc taactactat gtcacaagca caaatccctt cactatacct
     7561 cgaaaacaag acccctacct atgatgaaac cattgcctat ttcaagcata tagacagcat
     7621 gtgcgattac atgcagatgc ggtcctatgg caccactgat gcaggatttc cgcttgatgt
     7681 tttggtgatc gacggcgata aacaatttac acctgaggca gccagagcag caggaaaagc
     7741 aatactcctg atcaacaatg gcatacatcc cggcgaaccg gatgggatcg acgcttgtat
     7801 acaattgata acgcaatatg caggtgcagg cgatttgaaa tcagcgctcg aaggactggt
     7861 tatttgcatt attccggttt acaatatcga tggtgcctta aatcggaaca gcactacacg
     7921 cgtaaaccag gagggacctg agtcttatgg cttcagaggc aatggcagaa actacgacct
     7981 caaccgcgat ttcgtgaaat gtgatactcg taacagcaaa tcgtttgcag aaatctttca
     8041 cctgtgggat ccggatattt ttgtagacac ccacgtgagc aatggtgctg attatcaata
     8101 tgttatgaca ttaattccca ctcaacataa caaattgaca ccacccttgg gagccttact
     8161 gaagaacgat atgcttcctt ccttatacaa ggcgatggaa ggaaaagggt tccccatgtg
     8221 cccttatgtg aacgaagtag accgcatccc tgataacggt attgccggat ttctggacca
     8281 cggtcggtat tcaaccgggt ttacggcgct tttcaatacc ataggcttta tgccggaaac
     8341 gcacatgttg aaaccatacc cgcagcgagt ggcctcaacg ttggcattta tagaagtggt
     8401 tatcgattac atacaagaga accgtagcgc aataattgag gctcgggctg ttgcaaaaca
     8461 gactgtcatt caggcagata cgttcaattt acaatggaaa ttggatactg agcagcaaga
     8521 atcgtttatg ttcaacgggt atgccgccaa gttcaagaaa agtgatgttt caggtctcga
     8581 caggctctgg tacgaccgca atgagccctt cacgaagccg ataccctttt tcgacaccta
     8641 tacgcctacc ctatcaatcg ttgctcctga ggcttatata gtgccccagg catggttgcc
     8701 ggtgatagag cgattgcaat ggaatggtgt caggatggag cgactggaaa aggataccgc
     8761 catttcagtt gaatcttatt acatcaccgg ttttgactcg gttgacaggc cttgggaagg
     8821 gcattatttt cacagcaatg ttaaggttcg caaggttacg gaaacgctga actttatgaa
     8881 gggcgattac attattccgc tgaatcagga gtcgaacctc tacattgtgc aggtgcttga
     8941 acctcaggct ccggatgctt tttttgcctg ggggatgatg gatgccgtgt tgcaacaaaa
     9001 agaatggttt agcgactatg tgtttgaaga cctggcagcg gaaatattgc aacaggatcc
     9061 gcaactgaga aacatgctga atgaagcaat gattgcagac ccttcattga agaacgatgc
     9121 cgaaggccaa cttgcttttg tattcgaaca cagtaaatac aaagagccga cacataatcg
     9181 ttatccgata gcccgcatcg taaaaaaata gtaaagggtt catgaacaaa gatcaggcat
     9241 tcgaaattaa gtagttttac cgcatgcagg cagcattcaa aataggattt ttggaaatca
     9301 ccccggtgga tgtggttgat gtgttgctgg ttgcattcat tttatttcag ttataccgac
     9361 ttgtaagagg cagtctcgcc atcaacatat ttatcggctt attgatggtt tatgtgttga
     9421 gtttgattgc cgcagcatta gagctcacat taatgagcga tattctgggt gcatttatcg
     9481 atgtcggagt aattgccgtt attattgttt tccagcctga gatccgacgc tttttattgt
     9541 atgtaggaag aagcagtgat ttcagaaagg tgcgcttttg gaaggctttg agtcttcgaa
     9601 aaattcgaac cggagagtat aatcagaagg aagtaaatgc attaacagag gctattatgg
     9661 cattgtctga atcaaagaca ggcgcattaa tagtaattcc tcaaacatcg caactgggat
     9721 tttatcaaaa cacgggcata catctgaatg ccttgataac aaaggatctc atagaaagta
     9781 ttttctataa aaacagccca ttacacgatg gtgcgatgat catttctgaa caccggatag
     9841 cagctgtgaa atgtatcttg ccattatcag acaatccgga tcttccgggt aaactaggca
     9901 tgcgtcatcg aagtggtgta ggcattaccg aacagagtga tgctacctgc attattatta
     9961 gtgaagagaa tggcgaaata agttatgcgc gagaaggaaa attgtatcag caaatgcaaa
    10021 aggaagagat tcgcaaattg ctgagcaggg ctttatttca cagcgttaac tgattcaagc
    10081 gtatcaaccg gttttcctgc gcgttccata atagcagtaa ataatccatc agggtcgccg
    10141 ataaattcaa tttgaaggtc gatatttttc gagtaaggac tatcaggata tttctcttgc
    10201 aacgtgcgat aataggcaat tgcacgattg taatcgcgca gcaccaactc acaactattt
    10261 gctgccgtaa tcagtgcttg tggcactaat ggtgatgcgg gatattcttc aatcaaccta
    10321 tccagtaatt gcaattcttc ctttacagca ggcatttttt catataaacg cgctgcactc
    10381 atgaggtatt gagcacaaag cgtgtcttta ggatattggc gaacaaattc aaggtattcg
    10441 gtaatcagag cttcagcttc tactttatgt gcaactgtaa aaacaggatt agccttagcc
    10501 acgctatcca tttttacagc cattgcatct atctccttga tttctttacc ccgctgatca
    10561 caagaagcga atgtgcacgc aacaatcatt aacattaagc cataagctct attcatgata
    10621 tatctttatt ttagagccac aaatgtacta caatcaaacc ttagtcaagc tttttgccct
    10681 ggtggtgacc gccatttttg cgtcatcatg caatcggatc gtagttagat atgccggcga
    10741 aagtgacctt atacaagcct ctttacctgc acccggaaaa gtttgcaagg aatccgaaaa
    10801 ttatatcatc gataccaatc acctcaacgc aacacccatt cgctatgtgc gcgtcaattt
    10861 tcatatcatg caggaaagtg atggcagtgg aagttggaat gaggctgaag gtagaaaatt
    10921 tgttcacgac attgttgagc aaagcaataa ccgatggaac gataatcagc caatgacact
    10981 ccctctgggt aacagcacgc ctgatttacc aatccccatt cgcattgtct tgcaacgcga
    11041 tcccattaca ggtgaggaag gaatttattt tcaccgcgat gatactttat gttattggaa
    11101 tcgcagtgca acaaaaggtc ccaattcatt aagcgacaga acagtaatca gcaaatatca
    11161 agtaggcata gattcaatta ttaatatatt tctgattgaa cacgatcccg atagtattgg
    11221 cagtccgacc tacggagagc ccaaaatgtc aggaataagt tttattaaca gcatcaaatt
    11281 gttcagtatg tattatcagg gtcacatcgt gaaatacacc aatgccggtg tgccctttac
    11341 gcatgatgcc ttttattttt ctaaattgtt gaaccacgaa ttaggacatt gctttaattt
    11401 aaatcataca tggaattggg atgatagttg tgatgacacc ccaaaaaatt caggctgctg
    11461 gtctgttaca ggagtacccc cttgcgatac ccctaattca aataatatga tggattacaa
    11521 tatcagtcag caagctgtta ctccatgtca gattggtcgc atggaatata attttacaaa
    11581 agaaaccggt ggcgcaagac aatttgccat tccttattgg tgtgagtatc atgcatttga
    11641 acgcgtggta gtttatcgca atgaaaatct tgaatggaat ggtgcaaaag atctatgggg
    11701 cgatatagaa attcgtgacg gcgcaacact cactattcga tgcacggttt cacttcctga
    11761 gcaggctaaa gtaattatta aaccgggagg cacattgatc attgacggcg gcacattaac
    11821 aaatcgctgc ggtgataaat ttgaaggtgt tgaaatctgg gaaaataaaa aaacggatca
    11881 gatcggaaaa gttatgatca gtaatggcgg gaaattggaa aacatggtca attatacgac
    11941 tatcactaca acagaataaa caagcaatat gacgtttact tcagaacaat atcgatcatt
    12001 taaattattg tcacctgcag gatgcattat tgcattacta ggtttctttc ttacgtttac
    12061 tgaaatcaac tgcaatggca ccactttaga taccattaca ggcgttgaat tagctacagg
    12121 ttacagcccg gatatcgatc tgactgaatc taacgatgaa cctgaaacaa agcaggaaaa
    12181 atacgatccg aatgtgtttg cactcaatgc atggattgct gcaatgctgg gtttagcctt
    12241 atttcttatt ccaaaaatgc gcgatcagca tgccctccat ctcatcattg cattaattgg
    12301 attcggatgt atgatcgggc tcatgttcaa tttaaaaagt gcactcaacg atgctaaatc
    12361 ttcaggtggc gttgtgaatg tagaccttcc aattaccttc gatatgcagc cgggatattg
    12421 gattgttact gcatcttttt ttctgacatt ggttgctgac attgtgcttt ggatgcatca
    12481 acgaaaagta attgctgatt tgcatgcatc gaggcaaccc gataatgaat ccaatctatc
    12541 ataaaaaaaa gaggcagggt cctcacccta cctcttattg accaaaccta tacagaaatt
    12601 ttaattattc aaatccacga cgaatgatga gtcccactga gaaaggatgc aactcaggac
    12661 cttcaccttc ggcgaacatg ctattcagat cataagacgc atacacattg aaatcgccgt
    12721 aaccaatcac ataattcaat tcatgtctga actgtgcgag gttgaaatcg tcaaattcta
    12781 tatctttctt accatccagt ttatgttttg ttttaccgcg aagcattaag ccggggctat
    12841 agccgaattg catgcggaag ctgttttcag gatcccaagg ttttgtttca aacccgagga
    12901 gtaagggaat tgcaaggtgg gtggtattga atttattttt atcgtaatcc aatgtttctg
    12961 ttgccaatgt caatgaatcc atgtttggca ggatactgaa attctgatca tatctgaaat
    13021 gatgccattc gaggctcata ccataattga aatcgataaa tcctttagcc atatttaccc
    13081 ttgaacgcca cacgtgcaga tttacatcaa aacttcccca accatggagg tcttccattt
    13141 gtttgtattt atcttcgagt tgagttttat aatcagcatt taaataggta tt
//
LOCUS       DAOQHY010000092        13042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2328_length_13042_cov_5.650805, whole genome shotgun sequence.
ACCESSION   DAOQHY010000092 DAOQHY010000000
VERSION     DAOQHY010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 13042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13042
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2328_length_13042_cov_5.650805"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /locus_tag="PLL28_08735"
     CDS             complement(<1..1281)
                     /locus_tag="PLL28_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69447.1"
                     /translation="MKKIIYSLLCILLVQWHSRAQVVVFSDDFEGGLANWTYTGLWNT
                     TTAYAYSPTTCFTDSPDGNYPDMFSSEAAMNVSADLSTALDADVQFMALVDLETAFDY
                     VYVDVSDDAGASWINVYVLNGEGMFTWTNYTIPIGAFVGSPDVRVRFRFESDAAYNVD
                     GLYIDDFKITKYNIDVSPPLILHTPTNLYEGNLEETSLTAEIIDASGISSATLYYNVD
                     GGAFTAVPGDLIGGDTYSFIIPTEAPGSWIDYYIEAIDDFGVPNTATTETYQIIAGNY
                     IGYDDATIDFVADIGTLSLTGYVSAAVKVTLAGSTDVVTAIIQNYTDYMRPNNDIRFH
                     VWADDGGFPGADLVTPFYVTPEPTLDEPNKGTRIDLRGVPELEGIFGDVFIGYDVPGG
                     VAWISYTGTGIINRSYVQTAFGWSEFFGDFHFRVV"
     gene            1383..2198
                     /locus_tag="PLL28_08740"
     CDS             1383..2198
                     /locus_tag="PLL28_08740"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF69448.1"
                     /translation="MLKHPLFFAFLLTLYMPLHAQTADQWFATGYEKYEQQDYAGAIS
                     DYTECIMLAPDFPEAYYNRGLCNYFMGYYDAAIFDFNQVILQKPGEARAYYWKGYTQF
                     ALGHYEEAENAYSKALYYDPTYVAAFFERAFTRYTLGDFADAIEDFNKVLSIDHSNSD
                     AWFNRGLCFIAIADYTSALQDFSRVVESNAADPDAWYYIGFIYNKQLKFEEAVSALTK
                     VITLESDAVDALYQRGFAYYNLLQDEAACTDWSEGALLGNELCKELYEKECMK"
     gene            complement(2223..2822)
                     /locus_tag="PLL28_08745"
     CDS             complement(2223..2822)
                     /locus_tag="PLL28_08745"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF69449.1"
                     /translation="MRFVVLTLFVLQTAIVIGQNTEPITVSSDNDSVYFTMNGERSSI
                     ANTDMAAFYLEDGLVAANAGDFISAENKFRVGLLYDPENEQLWYNLGLAQYYQGAYED
                     AIYSFDAAVEVDPSNPENYNQRGLSKAMLGRYTDAETDFLILFKYAPDHPMGNFNYGI
                     LKLQMGDQATACTYLHKADSLGYENAPTVISTYCAMEGE"
     gene            complement(2956..4443)
                     /gene="accC"
                     /locus_tag="PLL28_08750"
     CDS             complement(2956..4443)
                     /gene="accC"
                     /locus_tag="PLL28_08750"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNF69450.1"
                     /translation="MNKILVANRGEIALRVMRTAREMGIKTVAVYSEADRNAIHTRFA
                     DEAYFIGPPQSNQSYLLMDKILEVAKMSGADAIHPGYGFLSENAVFAEKVAAAGIKFI
                     GPSAYSINMMGDKISAKQAAKNYHIPMVPGTDHAITDIAEAKAVAKKAGYPILIKASA
                     GGGGKGMRVVNNEEELEEQMHRAMSEALSAFGNDAVFIEKFVTSPRHIEIQVLADDHG
                     NVVYLFERECSIQRRHQKLVEEAPSAVVSPEMRKAMGESAVMVAKACNYSGAGTVEFL
                     VDDQLNYYFLEMNTRLQVEHPVTEFITGLDLVREQILIAEGKALSFTQDDLRIHGHAI
                     ELRVTAEDPTNNFLPDIGKLITYRRPQGHGIRVDDGYEEGMDIPIYYDPLLAKLIVHA
                     QTRELACKKMIRAIQDYQISGVATTLPFGTFVMQHEAFLSGNFDTKFIERYFTPEALQ
                     LHDEEASAIAAFLAARLMESEKSAETQSAIATQTTKSKWLERKRM"
     gene            4854..5516
                     /locus_tag="PLL28_08755"
     CDS             4854..5516
                     /locus_tag="PLL28_08755"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HNF69451.1"
                     /translation="MQFWPSVWGMLKKDLILEMRQKYAISGILLYMLCIVFIIFMTFG
                     QGVRGPVWVVLFWLIMLFTSVNAVAKSFLQESTGRLLYYYTIASPQAIILAKMLYNIG
                     LMILMSALALLMHSITSGYPIVDSPSFLLANVLGAIAFATTFSMISAIASKASNSGTM
                     MAILSFPILIPILIILIRISLNAIQQNNLAVNLQDLMYLAALDVMILAMALILFPYLW
                     RD"
     gene            5667..6359
                     /gene="ccsA"
                     /locus_tag="PLL28_08760"
     CDS             5667..6359
                     /gene="ccsA"
                     /locus_tag="PLL28_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNF69452.1"
                     /translation="MLKQLHNRWWKILCVAFLVYTFTAGLLIHTPDVPLLHETVRNLF
                     FHVPCWFSMLLLMLISLIYSIKFLRSGRMIHDVIAVSLLKVAMVFGVLGYLTGMLWGN
                     FIWGDLLSWITNDTKILGAAIGLLIYFAYFILRGSIDDEEKRARVAAVYSIFAFILLN
                     VSINVVPRLTSSLHPGSGGNATFTVYDLDNNLRMVFYPAVFGYTLLGLWISSIIIRMT
                     ILHYKKNDIPLV"
     gene            6356..6610
                     /locus_tag="PLL28_08765"
     CDS             6356..6610
                     /locus_tag="PLL28_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HNF69453.1"
                     /translation="MKKLTSLSRFIATFLFLLANITFAGAQNQGYATTADSNENLLRS
                     NGLIYVVVGVIVIILSGLILYLVSIDRKLGKMEKEYGDKH"
     gene            6666..8075
                     /locus_tag="PLL28_08770"
     CDS             6666..8075
                     /locus_tag="PLL28_08770"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNF69454.1"
                     /translation="MSNTSFFESVEKNFEKAAQITGHPRGLLDQIRVCNSVYHMKFPV
                     KIDGEFQVIEAWRVQHSHHRVPTKGGIRYDAMVNEDEVMALASLMTYKCAIVDVPFGG
                     AKGGIRINPRQFSSDQLERITRRYAAELCKKNMLGPGLDVPAPDYGTGEREMSWIVDT
                     YMALNPGSIDGYGCVTGKPVTQHGINGRREATGLGVFFGVREACSFADDMAELGLKPG
                     LEGKKVIVQGLGNVGYYSAKFFQDGGATIVGIGEYEGAIYNVNGLDVDAVFNHRKSTG
                     SILNFPGATNMANTAEILEQPCDILIPAALENVIHEGNAKKIKAKIIGEAANGPVTTE
                     ADDILNGMGVMIIPDMYLNAGGVTVSYFEWLKNLSHVRFGRLTKRFEQGSNMRIVDTI
                     EELTGKKLSQKEVDNIIKGPDEIDLVRSGLEETMINSYHAIRNEWKSNAASNNLRTAA
                     FVVAINKIASDYLAMGIFP"
     gene            8160..9023
                     /locus_tag="PLL28_08775"
     CDS             8160..9023
                     /locus_tag="PLL28_08775"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNF69455.1"
                     /translation="MRLRIMKYVGVMTLLLTISMSVGAQTITGTVHDIETGETLPFVT
                     IGIPGHGLGTVAQADGKFSLRTDGAGDNDTIRISMIGYATEKTTVGAWRKKSQVDVFL
                     SPIATQLDQVTVRPRDYKKVRLGNDYHTMTVQVGFNGGGEDTVEVNPPGAELGTIMKV
                     KDGRKYFLDSCGMNLTAFTPDSAVLRINFYQLQNDEPDMLLNKEPIYVTIYKNQSEVR
                     LDLRKYDIVAEDDFVMAAEWLVDLKGKEEKILFSGGFIGAGIRYRTNSASKWKKVPVG
                     IAMFVDAVYEK"
     gene            9073..10113
                     /locus_tag="PLL28_08780"
     CDS             9073..10113
                     /locus_tag="PLL28_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HNF69456.1"
                     /translation="MQSKQEKIASFDANGVGVANNQFFGLPFTYEESQLVLFSVPWEV
                     TVSYRAGTAKGPDAMLKASTQIDLYDNDNPNGWHNGIFTIKSSPTLKRRNTELRKIAG
                     TYIKALEQGKDMSLSGIPEKINEACATMVDQVYQQSKQILADGKIPALVGGDHSTPLG
                     LMKALAEHHGGFGILQIDAHADLRDAYEGFTYSHASIMFNALAIDNMKKLVQVGIRDL
                     CDAELQLIDNSDRRVVTYFNHEIKRAAYHGMTWGDICSRIVEELPKKVYVSFDIDGLL
                     QTFCPNTGTPVPGGLEFEQAVYLLNAIVESGRTIIGFDLVEVAPGEDEWDAIVGARML
                     YKMCNIALKGNA"
     gene            10106..10690
                     /locus_tag="PLL28_08785"
     CDS             10106..10690
                     /locus_tag="PLL28_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNF69457.1"
                     /translation="MLNLRNIIVLIAICISIGCNQDHGISQIDLYDRNDKAQQISFDN
                     YDATVITFLGPECPLSENYTKTINDLDSTFKSNVHFINIFPGTFYSKEAIDSFMHTYN
                     VMQNCLLDADFDLVRELHATITPESFVFDRNGKLVYSGAIDNWAVDLGQKRQVITEFY
                     VRDVLNAMLQHKEIPYTKTTAVGCFIETEAKQHH"
     gene            10693..12087
                     /locus_tag="PLL28_08790"
     CDS             10693..12087
                     /locus_tag="PLL28_08790"
                     /inference="COORDINATES: protein motif:HMM:NF015657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69458.1"
                     /translation="MRNLILFAFLALLMTSCADKSGSNKKQADEEKVILPDVVTFNEH
                     IAPIIFENCTPCHRPHSAGPFPFTTYDEIKAKAKTISKVTQSRFMPPWPADPTYTNFK
                     GERILTVTEIAMIKKWVEDDCPVGDVAKIPAVPHYPEGSQIGTPDLVINFPDFTLEGN
                     NKDHFFLVKVPFEIPEDKYVKTIEFIPGNKKLAHHMNANLIEYQPGKKKTVFNGKKII
                     PTNLAESADVVQTEMDNKNDDGTYPVLVPLVCNYLPGVSPAMYPEGIGGFRMTKQGTF
                     FINDFHFGPTPKDAIDTSSHFNIFFDDKPPVRPTYEILLGSLGVAPVTPALVIPPNEV
                     KTFTIDYQVQETMSLLTVNPHMHLLGKSFLAYALTPQMDTIPLIRINKWDFRWQYFYT
                     FKSVVVIPGGSRIHVEAVFDNTVNNVNNPYYPPQVIIDRGDRFDSMKTTNEMLQLILT
                     YMEYQSGDEYLSLE"
     gene            12089..>13042
                     /locus_tag="PLL28_08795"
     CDS             12089..>13042
                     /locus_tag="PLL28_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF69459.1"
                     /translation="MRKLFPYITRPILVLSLVSLFTDMASEMLYPVMPAFLESIGFSI
                     LLIGLLEGLAEMIAGLSKGYFGKRSDIQQKRLPFVQLGYALSAISKPMMALLSYPLWI
                     FFARTTDRVGKGLRSAPRDAMLNANASKETKASVFGFHRSMDTIGAILGPLLALIYLQ
                     CYPQDYKNLFLLAFIPGLIAVIFTWSLKEEKKAARGERVKFFSFLSYLPNAKKPYRQL
                     VIGLLAFALVNSSDVLLLLRMKQIGIDDVTIISMYIGYNIIYALLAFPIGILADKLSI
                     KYIFIAGLLIFAIVYTGFAYAESVTTYYILFACYGIYAAATE"
BASE COUNT         3722 a         2880 c         2735 g         3705 t
ORIGIN      
        1 aactacacgg aaatggaagt cgccaaagaa ctccgaccat ccgaaagctg tctgtacata
       61 acttctgttg atgatacctg ttcctgtgta cgaaatccat gccacacctc cgggtacatc
      121 gtatccgata aatacatcgc caaaaatccc ttcaagctca ggcacaccgc gcagatcaat
      181 gcgtgttcct ttattcggtt catccaatgt aggttcagga gttacataaa atggtgtcac
      241 aagatcagca cccggaaaac caccgtcatc agcccatacg tggaatcgga tatcattatt
      301 cggacgcata taatcggtat aattttgaat gatggcagtt accacatcag tagaacctgc
      361 taatgtcact tttactgccg ccgaaacata acccgtaaga ctgagtgtcc cgatgtcggc
      421 aacaaaatcg attgtggcat catcgtaacc aatataattt cctgcaatga tctgataggt
      481 ttcggttgtt gccgtattag gcacaccgaa atcgtcaatt gcctcaatat aataatctat
      541 ccatgaaccg ggcgcctcgg taggtatgat gaaagaatag gtatctccgc cgatgaggtc
      601 accgggaact gcagtgaagg cacctccatc aacattataa tataaagttg cagatgaaat
      661 tcctgaagca tcaatgatct cggcagtgag tgatgtttcc tctaaattcc cttcatacaa
      721 attagtaggt gtatgcaaaa tgagcggtgg cgaaacgtcg atattatatt tcgtgatttt
      781 aaaatcatca atataaagac cgtcgacatt ataggcggca tcactctcga atcgaaacct
      841 tacgcgcaca tcgggactac ccacaaaggc acctatcggt atggtgtaat ttgtccatgt
      901 aaacatgcct tctccattca gcacataaac attgatccag gaagcgcctg catcatctga
      961 tacatcgaca tatacataat caaatgccgt ttccagatca acgagcgcca taaattgcac
     1021 gtctgcatcc aaggccgtgc tgaggtcggc actcacattc atggctgctt ctgagctgaa
     1081 catgtcggga taatttccat ccggactgtc tgtaaaacag gtcgttggac tgtaggcata
     1141 ggccgtggta gtgttccata atcctgtgta ggtccagttg gcgagaccac cctcaaaatc
     1201 atcggaaaaa acgacaacct gagcgcgtga atgccactgt acgaggagga tacaaaggag
     1261 cgagtagata atttttttca tttgtttagt tgttggatcc atcactaaat tactcaaaaa
     1321 ttataagttg gcaagctatt cgctgtatag ctgttacctt tgcaccgtta acaatcagcc
     1381 aaatgttaaa acatcctctt tttttcgcct ttctgctcac tctgtatatg ccattgcatg
     1441 cgcaaactgc cgatcagtgg tttgctaccg gatacgaaaa gtatgaacaa caggattatg
     1501 caggtgccat cagcgattat accgaatgca ttatgttggc accggatttt ccggaagcat
     1561 attacaatcg cgggctctgc aattatttta tgggctatta cgacgcggcg atcttcgatt
     1621 ttaatcaggt gatattacaa aaaccgggtg aagcacgcgc ctattattgg aaaggttata
     1681 cgcaatttgc tctcggtcat tatgaagaag cagagaatgc atattcaaag gcattgtatt
     1741 acgatcctac ttatgtagca gcattttttg aacgcgcatt taccagatac acacttggcg
     1801 attttgctga cgcgatagaa gattttaata aagtattatc catcgatcac agcaacagcg
     1861 atgcatggtt taatcgcgga ttgtgcttta ttgctatcgc tgattacact tctgcattgc
     1921 aagatttttc acgcgtagtg gaaagcaatg ccgctgatcc ggatgcttgg tattacatag
     1981 gatttatcta caataaacaa ttaaaattcg aggaagctgt aagcgcactt acaaaggtga
     2041 taacactcga atctgatgcc gttgatgctt tatatcagcg cggatttgca tactacaatt
     2101 tattgcagga tgaagctgct tgcaccgact ggagtgaggg cgctttgttg ggaaatgaac
     2161 tctgtaaaga attgtatgaa aaagaatgta tgaaataatt gtggtgtaaa ttttctacct
     2221 ctctattctc cttccatagc acaatacgtt gaaataacgg tcggcgcatt ttcataaccc
     2281 aggctatcgg ctttatgcaa ataagtacac gcagttgcct gatcgcccat ttgcaatttt
     2341 aaaatgccat agttgaaatt tcccatcgga tgatcgggag catatttaaa taaaatcagg
     2401 aagtctgttt cagcatcggt gtatcgccct aacatagcct ttgacaatcc acgttgattg
     2461 taattttcag gattggaagg atctacctcc acagctgcat cgaaactata aatggcatcc
     2521 tcataagcac cctggtaata ttgcgccaat ccgagattat accataattg ttcattctca
     2581 ggatcgtaca ataatcccac gcgaaattta ttttcagcac tgataaaatc tccggcattc
     2641 gcagcaacca gtccgtcttc gagataaaag gctgccatgt cggtattggc aattgacgat
     2701 cgctctccgt tcatggtgaa atacacggag tcgttatccg acgataccgt aattggctca
     2761 gtattctgcc ctatgacaat tgctgtttgc agcacaaaaa gtgttaatac gacgaatctc
     2821 ataaccataa aacgcatttt ttactcagta atgggtacaa caggagcaca aaatctcctc
     2881 aaaattgtgt gcgtaatccg gtaggtcgtc tatcatgcaa tttaacatat cgcatgaaaa
     2941 ataaatcatc agcaattaca tgcgtttgcg ttccaaccac ttgcttttag tggtttgggt
     3001 ggcgatggca ctttgggttt ctgctgattt ttcggattcc atcaatcgcg cagctaaaaa
     3061 tgccgcaatc gccgatgctt cctcatcgtg aagctgcaat gcttcgggtg taaaatatcg
     3121 ttcaataaat ttagtatcga aattcccact caaaaatgcc tcatgttgca tcacgaaagt
     3181 accaaaaggt aaggtagttg caacgcctga tatctgataa tcctgaatgg cgcgaatcat
     3241 ttttttacat gcgagttcac gcgtttgtgc atgcacgatt agtttcgcca gcaacgggtc
     3301 ataataaata ggaatgtcca taccctcttc atatccgtcg tcaactcgaa tgccatgtcc
     3361 ctgaggtcgg cgataagtga tgagtttacc gatatcagga aggaaattat ttgtaggatc
     3421 ttctgccgtt acgcgcaatt caatggcatg tccatgaatg cgcagatcat cttgtgtgaa
     3481 tgaaagtgct ttgccttcgg cgatgagaat ttgttcgcgc acaagatcaa gaccggtgat
     3541 aaattctgtt accggatgct ctacctgcaa gcgtgtgttc atttccagaa aataataatt
     3601 caactgatcg tcgacaagaa attctactgt tcctgcacct gaataattac atgcttttgc
     3661 aaccatcaca gcactttctc ccattgcttt ccgcatctcg ggagaaacaa ctgcgcttgg
     3721 cgcttcttcc actaatttct ggtggcgacg ttgaatagaa cattcgcgtt caaataaata
     3781 caccacatta ccatgatcat cagccaacac ttgaatttcg atatgtcgcg gtgaggtgac
     3841 aaatttttcg atgaatactg catcattccc aaaagcagag agtgcttcac tcatagcgcg
     3901 atgcatttgt tcttcaagct cctcttcatt atttacaaca cgcattcctt ttccaccacc
     3961 gccggcactc gccttaatta atatcggata acctgctttt ttagcaactg cttttgcttc
     4021 cgcaatatcg gtaatcgcat gatcggttcc cggaaccata ggaatatgat agtttttcgc
     4081 tgcctgctta gcagagattt tatcacccat catatttatg ctgtatgcac tcggaccaat
     4141 aaatttaatt cccgctgccg caactttttc tgcgaatacc gcattctcac tcaaaaatcc
     4201 ataccccgga tgtatcgcat ctgctccgct catttttgca acttcaagaa tcttatccat
     4261 caatagataa ctctgattcg attgcggcgg accgataaaa tacgcttcat cggcaaatcg
     4321 ggtatgtatc gcattcctat ctgcctctga ataaacagct actgttttga tgcccatctc
     4381 tctcgccgta cgcataactc gcaaagcaat ctctcccctg ttagccacta atatcttgtt
     4441 gatcttcata gcaacaaagg tggtgaagga gttttgtttt tgcaagaagg atggaatgtg
     4501 ggaatttggg aatcccgctt gggcgggatg tcgctgcgct ccatgtggga attcattgct
     4561 acttcaattt ggctgcacaa ttgcatatgg agtacagtaa cagcccgttc agcgatactt
     4621 gcacacttgc acacccgcac acctgcacac ttccacacta ttaatgggaa tgtgattggt
     4681 tcgcgcaaag tggcaaagct gcaaagtcgc aaatgataaa tctcaccaac acactcacca
     4741 ctcacccact gacccactga cccactcacc cactcaccac tcacccactc acccactcac
     4801 ccactcctct ctcctctctc ctttctcctc tctcctttat ctatctttgc cccatgcagt
     4861 tctggccctc cgtttgggga atgttgaaaa aagatctcat ccttgagatg cgtcagaagt
     4921 atgccatcag cggaattctg ctgtatatgc tctgcatcgt tttcatcatt ttcatgacct
     4981 tcggacaggg cgttcgcggt cccgtttggg tggtactgtt ctggctgatc atgctgttta
     5041 catcagtaaa tgccgtcgcc aaaagctttt tacaggaaag caccgggcgt ttgctctatt
     5101 actacactat tgcctcaccc caagccatca tcctggccaa aatgctgtac aacatcggtt
     5161 tgatgatatt gatgtcggca ttggctctct tgatgcacag cattacgtcc ggttatccga
     5221 ttgttgatag tccctcgttt ttgcttgcca atgtgctagg tgctattgct tttgccacca
     5281 ccttcagcat gatttccgcc atcgcctcta aagcatcgaa cagtggcacc atgatggcga
     5341 ttttaagctt cccgatactg attccaatac tgatcatcct cattcgcatt tcgctgaacg
     5401 ccatacaaca aaacaatctg gcagttaacc ttcaggacct gatgtacttg gctgccttag
     5461 acgtgatgat cctggctatg gccttgatcc tgtttcctta tctgtggcgc gattgacatc
     5521 cgcaaagcgc ttatagattt tcagaatact acattttttg accttgtctg acttggttaa
     5581 aatggcaata cccttgttaa ttattactta tctttgcacc caattcgcag gctaccccac
     5641 ctgagggtgg aaatgaggta atcaagatgc tcaaacaatt gcacaaccgc tggtggaaga
     5701 tactttgtgt cgcctttctg gtttacactt ttaccgcagg acttctcatt catacacccg
     5761 acgtgccttt attgcatgaa actgtccgca acctgttttt ccatgtgcca tgctggtttt
     5821 caatgctgtt attaatgctg atatcactga tttacagcat taaatttttg cgttccggac
     5881 gcatgataca tgatgtgata gcggtttcac tgcttaaagt agcaatggtg tttggcgttt
     5941 taggatatct gaccggtatg ctttggggca attttatttg gggtgatttg ctaagctgga
     6001 tcactaacga cactaagatt ttaggcgctg cgatagggct tttgatatat tttgcctatt
     6061 tcatcctcag aggatctatt gatgatgaag aaaaacgtgc cagagtagcc gccgtataca
     6121 gcattttcgc atttatcctg ctcaacgttt ccatcaatgt ggtacctcgc ctgaccagca
     6181 gcctgcaccc cggatcggga ggaaatgcca cctttacggt ttacgacctc gacaataact
     6241 tgcgcatggt attctaccct gctgtgtttg gatatacctt gctgggattg tggatcagca
     6301 gtatcatcat ccgcatgacc attctgcatt acaagaaaaa cgacattccg ctcgtatgaa
     6361 aaaactgacc tctcttagcc gttttatagc cacatttctt tttttgctgg caaacattac
     6421 ctttgcgggt gctcaaaacc aaggttatgc gaccacagcc gatagcaacg aaaatctgct
     6481 gcgcagcaat ggtctcattt acgtggttgt aggcgttatc gttatcattc tttcaggctt
     6541 gattctttac ctggtgagca ttgaccgcaa actgggcaag atggaaaaag aatacggcga
     6601 taaacattga acatttttat aaccaagtat tcattattgt aattaaactt acaaactcaa
     6661 taagtatgtc aaataccagt ttctttgaaa gcgtagaaaa aaactttgaa aaagctgcgc
     6721 agatcacagg tcaccctcgc ggcctcctcg accaaatcag ggtgtgtaac agcgtttacc
     6781 acatgaaatt ccccgtgaaa atcgacggcg aattccaggt aattgaagca tggcgcgtgc
     6841 agcatagtca ccaccgcgtt cctaccaaag gtggtatccg ctacgatgcc atggtgaatg
     6901 aggatgaagt aatggcattg gcgagtctga tgacttacaa atgtgccatc gtagatgtac
     6961 cattcggtgg tgcaaaaggt ggtatccgca tcaatccacg acagttctca tctgatcaat
     7021 tggagcgcat cacacgtcgc tatgcagctg aactttgcaa aaagaatatg ttaggtcccg
     7081 gcctcgatgt tcctgctccc gattacggaa caggtgaacg cgaaatgagc tggatcgtag
     7141 atacctatat ggctttgaat ccgggtagta ttgacggtta cggttgcgtt accggaaaac
     7201 ctgttacaca acacggtatt aacggtcgcc gcgaagctac aggcctcggc gttttcttcg
     7261 gcgtgcgcga agcttgttca tttgccgatg atatggcaga actcggtttg aaaccgggcc
     7321 tcgaaggaaa aaaagtaatt gtgcaaggtc ttggtaacgt aggttactat tctgcgaaat
     7381 tcttccagga tggtggcgct actatcgtag gtattggcga atatgaaggt gctatttata
     7441 atgtaaatgg cctcgatgta gatgcagtgt tcaatcaccg caaatctaca ggcagcatcc
     7501 tcaatttccc gggtgctaca aacatggcta ataccgccga aattcttgag caaccttgcg
     7561 atatcttaat tcctgctgca ttggaaaatg taatccacga aggcaatgcg aaaaagataa
     7621 aagcaaaaat tatcggtgaa gctgctaacg gccctgttac cactgaagct gacgatattc
     7681 tcaatggcat gggtgtgatg attatacctg atatgtatct caacgccggt ggtgttaccg
     7741 tgagctactt cgaatggttg aaaaacttat cgcacgtgcg tttcggacga ttgacaaaac
     7801 gtttcgaaca aggcagcaac atgcgtattg tagatactat tgaagaattg accggaaaga
     7861 aattatcaca aaaagaagta gataatatca tcaaaggacc ggatgagatc gacctcgttc
     7921 gctcaggtct ggaagaaaca atgatcaatt cttatcacgc catccgcaac gaatggaaat
     7981 caaatgctgc aagcaacaat ttgcgcacag ctgccttcgt tgtagcaatc aataaaattg
     8041 caagcgacta tctggcaatg ggtatcttcc catgacggag accgcataga aaagaagact
     8101 cctgctcaca aagcaggagt tttttatttt cgaccatatc tgtaacattt tatgctctca
     8161 tgcgccttag gattatgaag tatgttggag tgatgacact tttgctgact atcagtatgt
     8221 cagtcggcgc acaaacaatt accggcacgg tccacgatat agaaaccgga gaaacattgc
     8281 cttttgttac tattggtatt cccggacatg gcttgggaac agtagcacaa gccgatggga
     8341 aattttcttt gcgcacagat ggtgcaggcg ataatgatac cattcgcatc agtatgattg
     8401 gctatgcaac ggaaaaaact actgtaggtg cctggagaaa aaaatcgcag gtagacgttt
     8461 ttttgtcgcc gatagccact caacttgatc aggtaactgt gcgaccacgc gattataaaa
     8521 aagtacgact tggcaatgat tatcacacca tgaccgtgca agttggattt aatggcggcg
     8581 gagaggatac cgtggaagta aaccctcccg gagctgagtt aggaaccatc atgaaagtaa
     8641 aagacggcag gaaatatttc ctcgattctt gcggcatgaa tctgaccgca tttacaccgg
     8701 atagtgctgt tttgcgcatc aatttttatc aattgcagaa tgacgaaccg gatatgttac
     8761 tcaataaaga accaatttat gtaacgatat acaaaaatca atctgaagtg cgtcttgatc
     8821 ttaggaagta cgatatcgtt gcagaagatg attttgtgat ggctgctgaa tggcttgtcg
     8881 accttaaagg caaggaggaa aaaatattgt tcagcggtgg atttattggc gccggaatac
     8941 gctatcgcac aaacagtgca tcgaaatgga aaaaagtgcc ggtcggcatt gccatgtttg
     9001 ttgatgccgt ttacgagaaa tgatgtggcg cacaggttgc agtggtaagc agcggcttgc
     9061 tatctttgca acatgcaatc aaaacaggaa aagatagcat cctttgatgc caatggtgtt
     9121 ggtgtagcca acaatcaatt tttcggactt ccatttactt acgaagaaag ccaattggtg
     9181 ttattctcag tgccatggga agtgacagtc tcttatcgcg ccggcaccgc caaaggacct
     9241 gatgccatgc tcaaggctag cacacagatc gatctctatg acaacgataa tccaaacggc
     9301 tggcataatg ggattttcac catcaaatca tcgccaacac taaaacgtcg caacacagaa
     9361 ttgcgaaaaa ttgcaggcac ttatatcaag gctttagaac agggaaaaga tatgtcgctt
     9421 tcaggtattc ctgaaaagat aaatgaagcc tgtgcaacaa tggttgatca ggtttatcag
     9481 cagtcgaaac aaattcttgc cgacggaaaa attcctgcgc ttgtgggagg tgatcacagc
     9541 acaccactcg gactcatgaa agcactcgca gaacatcacg gcggattcgg cattctgcaa
     9601 attgatgcac acgccgatct tagagatgct tatgaaggat ttacctattc acatgccagc
     9661 atcatgttca atgcgctcgc tatcgataat atgaaaaaac tcgtgcaagt gggtattcgc
     9721 gatctgtgtg atgccgaatt acaacttatc gataattctg atcgtcgcgt tgtaacctat
     9781 tttaatcatg aaataaaacg cgcagcatat cacggaatga catggggcga tatttgctcc
     9841 cgcattgttg aagagcttcc gaaaaaagta tatgtcagct tcgatatcga tggcttactg
     9901 caaacatttt gtccgaatac aggcacacct gttcccggtg gacttgaatt tgaacaggct
     9961 gtttatttat taaatgcaat tgtcgagtct ggccggacaa tcatcggatt tgacctcgtg
    10021 gaagttgccc ccggagaaga tgaatgggat gccattgttg gtgcacgtat gctgtataaa
    10081 atgtgcaata ttgcgttgaa aggaaatgct taatctgcga aatatcatcg ttcttattgc
    10141 aatttgtatc tcgataggat gcaatcagga ccatggtata tcacaaatag acctttacga
    10201 tcgcaacgat aaggcacaac aaatttcttt tgacaattat gatgcaactg tcattacatt
    10261 tctgggacct gaatgtccat tgagtgaaaa ttacacgaag acaatcaatg acctggatag
    10321 tacttttaaa agtaatgtgc atttcatcaa tatttttccg ggtacatttt atagcaagga
    10381 agctatcgac agttttatgc atacttacaa cgtaatgcag aattgcttat tagatgccga
    10441 ttttgatctc gttcgtgaat tgcacgcaac cattacacct gaatcatttg tgttcgacag
    10501 gaatggaaaa ctagtgtata gcggagcgat agataattgg gctgttgacc tgggacaaaa
    10561 gcggcaggta atcactgagt tttatgttcg cgatgtattg aatgccatgc tgcaacataa
    10621 agaaattccg tataccaaaa ctacagctgt aggatgtttt attgaaacag aagcaaaaca
    10681 acatcattga acatgcgcaa tctcatacta tttgcttttc ttgcattgtt gatgacgtca
    10741 tgcgccgata aaagtggttc aaacaaaaaa caggcggatg aagaaaaagt aatattgccg
    10801 gatgtagtta ctttcaatga acacattgca ccaatcatat tcgaaaactg caccccgtgt
    10861 catcgcccac atagtgcagg tccatttcca tttactacgt atgatgaaat aaaagcaaag
    10921 gcaaaaacaa tttcaaaagt aacccaatcg cgcttcatgc cgccttggcc tgcggatcct
    10981 acttacacta atttcaaggg tgaacgcatc ttaacagtta cagaaattgc gatgataaaa
    11041 aaatgggtgg aagatgattg ccctgtgggt gatgttgcga aaattcctgc tgtcccacat
    11101 tatcctgaag gttcacaaat aggcacacct gatctggtga tcaattttcc ggatttcaca
    11161 ctggaaggaa ataataaaga ccatttcttt ttggtgaagg tgcctttcga aatacctgaa
    11221 gacaaatatg tgaaaaccat tgagttcatc ccgggaaaca agaaacttgc acatcacatg
    11281 aatgcaaacc tgattgaata tcaaccggga aaaaagaaaa cagtattcaa tggtaaaaaa
    11341 attattccga caaatttagc tgagagtgcc gatgtagtgc aaactgaaat ggataataaa
    11401 aatgatgatg gcacctatcc ggtattagtt cctttggtat gcaattatct ccccggtgtt
    11461 tctccggcaa tgtatccgga aggcataggc ggtttcagga tgacaaagca aggaacattt
    11521 tttatcaacg acttccattt cggacctact ccgaaagacg caatagacac ttcatcgcat
    11581 tttaatattt tcttcgacga caaaccacct gtgcgaccaa cctatgaaat attattaggt
    11641 tcactcggtg tggctccggt aacccctgca ttggtaattc cgccaaatga agtaaaaact
    11701 tttactatcg attatcaagt acaggaaacc atgagtttgc tcaccgtaaa tccgcatatg
    11761 catcttttgg ggaaatcatt tttagcatat gcgcttaccc ctcagatgga taccattcca
    11821 ttgatacgta tcaataaatg ggattttcgc tggcagtatt tttatacatt taaaagcgtg
    11881 gtggtgatac ccggcggctc tcgtattcat gtagaagctg tatttgataa taccgtgaat
    11941 aatgtcaaca atccttatta tcctccacag gtgattattg acagaggcga tagatttgat
    12001 tcgatgaaaa ctaccaacga aatgttgcaa ttgatcctca catacatgga gtatcaatcg
    12061 ggcgacgaat acttgagcct cgaataacat gcgaaaatta tttccatata tcacacgacc
    12121 aattctggta ctgagccttg ttagtctctt taccgatatg gcgagtgaaa tgctttatcc
    12181 tgtcatgcct gcatttcttg aaagcatagg attcagcata ctgctgatag gattattgga
    12241 aggccttgct gaaatgatag cgggacttag taaaggatat tttggcaaac gctcggatat
    12301 tcaacaaaaa agattgccct ttgtccaatt aggttatgca ttaagtgcca tttccaaacc
    12361 catgatggca ttactcagct atccgctttg gatatttttt gcgagaacga ccgaccgcgt
    12421 tggaaaaggt ttgcgttcgg caccgcgcga tgcgatgttg aatgcaaatg cgtcgaagga
    12481 aacaaaagcc agcgtattcg gattccatcg ctccatggat accattggtg ctattctcgg
    12541 acctttgctc gcattgatat atctgcaatg ctatcctcaa gattataaga atttatttct
    12601 cctggcattt attcccggat tgatagcggt aatatttact tggtcactca aggaagaaaa
    12661 aaaagcggca agaggtgaac gcgtaaaatt tttttctttt ctctcctatt taccgaatgc
    12721 aaaaaaacca tatcgtcagt tggtaatcgg tctattggca tttgccttgg taaatagttc
    12781 cgatgtgcta ttactcttgc gcatgaaaca aatcggtatt gatgacgtga ccattatatc
    12841 aatgtatatc ggatataata tcatttatgc attattggca tttcctattg gcatattggc
    12901 tgataagttg agcatcaaat acattttcat cgccggttta ttgattttcg caattgtata
    12961 caccggattt gcatacgccg aatctgtaac cacatactat atactatttg cttgctatgg
    13021 catatatgcc gctgcaacag ag
//
LOCUS       DAOQHY010000093        12999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2345_length_12999_cov_5.752704, whole genome shotgun sequence.
ACCESSION   DAOQHY010000093 DAOQHY010000000
VERSION     DAOQHY010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12999
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2345_length_12999_cov_5.752704"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLL28_08800"
     CDS             complement(<1..283)
                     /locus_tag="PLL28_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69460.1"
                     /translation="MTHNYQTASPVKRISAFAIDTILVGVVSAAFLFVTMGKTPAKLQ
                     ASGDEGRPSIADKYQFFAYAEPIVNDPNRAIYIENFLKKNKMQAMVGLFL"
     gene            421..2211
                     /gene="lepA"
                     /locus_tag="PLL28_08805"
     CDS             421..2211
                     /gene="lepA"
                     /locus_tag="PLL28_08805"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNF69461.1"
                     /translation="MEHIRNFCIIAHIDHGKSTLADRLLEVTKTVSEREAQAQVLDDM
                     DIERERGITIKSHAIQMDFRYEGKQYVLNLIDTPGHVDFSYEVSRSIAACEGALLLVD
                     ATQGIQAQTISNLYLAIEHDLEIIPVVNKIDMDGAMVDEVKDQIVDLIGCKREEILEA
                     SGKTGVGIDSIFDAIIHRIPSPKGNVDAPLQALIFDSVFNSYRGVVAYYRIFQGTIKK
                     GDKIKFVNTQNNYEANEVGILRLQYEPRSEVKAGDVGYVITGIKEAKEIKVGDTITLR
                     DNPCSNEVAGFKDVKPMVFAGIYPIDNDDFADLRDSLEKLQLNDASLVFEPESSVALG
                     FGFRCGFLGLLHLEIVQERLEREFDQNVIITVPNVSFDCYTTAGEKIVVHNPTDMPDP
                     GSIDKIEEPYIRSQIITAPDYIGAIMKLCMDKRGILKNQVYLTTNRVELTWEMPLAEI
                     VFDFYDKLKSISRGYASFDYELIDYREGDLIKLDIKLNGEQVDAFSALIHRDKAYDFG
                     SRLCGKLKDLLPKQQFVIAIQAAIGAKIIARETISAMRKDVTAKCYGGDISRKRKLLE
                     KQKKGKKRMRQIGTVEVPQEAFLAVLKLNE"
     gene            2313..3200
                     /locus_tag="PLL28_08810"
     CDS             2313..3200
                     /locus_tag="PLL28_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HNF69462.1"
                     /translation="MAQPIILDGKKLSTEIQDEIRKKIDIRRTAGLKIPHLAAVLVGN
                     DGASTTYVNSKIRLCKSVGMQSTLIHLDASTSEEELLRVVHDLNTNPDIDGFIVQLPL
                     PKHIQEMHIVEAIDPSKDVDGFHPVNLGRMQLGLPAFISATPAGIMTMLSRYHIDTAG
                     KHCVVIGRSNNVGTPMSLLLSRNTNPGNCTVTLCHSRTKNLTEICRSADIIIAALGKP
                     KFVTGDMVKEGAVVIDIGISRVDADNEKGYLILGDVDFPNVAPKCSAISPVPGGVGLM
                     TVVSLIMNTMQAAESRDKL"
     gene            3234..3830
                     /locus_tag="PLL28_08815"
     CDS             3234..3830
                     /locus_tag="PLL28_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNF69463.1"
                     /translation="MELPVMEQFYTIQGEGFHQGRAAYFIRLGGCDVGCVWCDVKESW
                     DVDAHPKLSVSSLVNNAAAFASKIVVITGGEPLMHNLSALTNELHEKGLNTHLETSGA
                     HPLSGIWDWITFSPKKFKSPLASVAAQANELKVVINHKSDLVFAEMHAATVSESCLLY
                     LQPEWEKRDSMMPLITEYVRTNPQWRISLQTHKYLQIP"
     gene            complement(3882..5936)
                     /locus_tag="PLL28_08820"
     CDS             complement(3882..5936)
                     /locus_tag="PLL28_08820"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HNF69464.1"
                     /translation="MKNMLKHLRTGLVLAAVIGSSIAAKAQYCTPTYTTGTAEGDYIN
                     GVTVGTIANTFSGAAITGVGYSDYTFLSTDLNIGDAYTMTCNNNPSWSQTYTAWIDYD
                     HDNTFETSEVLGNLSLSASATGSFSFTVPVTAMSGTTRMRVRCIYPSGLVTPLDPCAL
                     ATYGETEDYTVNLLGGAMNDVGVIDITNPTTGPGLGLEDVIVTVRNYGSEPASGFTVS
                     YQVDGGLVTAGFYGGTIAPFSTGSWTAPEGWDFSAEGCYNITAWTTLAGDEDPGNDSY
                     TEEVCHTGAVTGTGAIYMLSNTTGGEPWFTTNNSTAMTTVFGAEGTGWNKNYYETAPA
                     ATVFTPDNCFVFMEGSDGHANELETYLTANMAVIESWVSSGGKLLLNAAPNEGDGMSF
                     GFGGTSLVYSYFTSTANGVGGHPIFSGPYTPAGTTYTGTSFGHARITGTGLTNVMVDA
                     AAPSNVVLAEKTWGGGIVMFGGMTTDNWHDPDPNAANLRANIFSYLSCEVVTDCPAPV
                     GLGADAITGTSAHLFWDPSLNSGGYHLSVYKTDETLVLKKKVLGGATDWTVTGLTPGT
                     DYAFHVRSICTGLGMASDISYHYYFTTPARLGDENTNVVLFPNPNTGTFDIALNGYEG
                     QQVNIQIVSALGQTVYNNTVNVNATGFTQNVNLNNAAAGMYYVNILTDKGVMNYQVVI
                     TD"
     gene            complement(6219..8276)
                     /locus_tag="PLL28_08825"
     CDS             complement(6219..8276)
                     /locus_tag="PLL28_08825"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HNF69465.1"
                     /translation="MMNMLSYAKTWLTLGVVVVTTGLASAQYCTPVYTTGTADGDYIN
                     GVTVGAIANTGSGAAITGVGYSDYTALSSDLTVGSTYTMTLDNNPSWSQTYTAWIDYD
                     QDEVFETTEVLGGVGLSAGASGTVTFTVPVGTPGGSTRMRVRCIYPSGLAIPQDPCLS
                     ATYGEAEDYTINIVGGAANDVGVIDITNPISAVDLGFENVTITIRNYGSEPASGFNVY
                     FQVDGGLVTGATYGGTIDAYSTASYTFGDTWDFTADGCYDITAWTDYELDEIADNDDY
                     TETVCNLGPITGTGAWYIYSNSTGGEPWFTTTNSTAMTTVFGAEGTGWNRAFFETLDP
                     LSIFNSDNCFVFIDGSDAQANELETFLTANMSTIENWVSSGGKLLLNSAPNEGDGMSF
                     GFGGTTLTYSYYTSNATAVDALHPIFNGPFTPAGTDYTGTSFGHAIVSGVDLSNVMVD
                     LFNPSAVVLAEKTWGDGRVMFGGMTTNNFHSPLTNAANLRANIIAYMSCMEFVICLPP
                     GGLYADGITTDDAVLHWDVVDGALNYNVILQNTATGLLKKKVFATNMAVIENKLSPLT
                     NYAFRVKTGCGVDPDIVSMASEWYYFTTLGREGVNEAAVSLYPNPNNGTFTINVAGYE
                     GNSFNLNVVSATGQIVYTKTVDVTGANYAEQIALDNIPAGMYQVNLSNAQYQINYAIV
                     VTK"
     gene            complement(8539..10407)
                     /locus_tag="PLL28_08830"
     CDS             complement(8539..10407)
                     /locus_tag="PLL28_08830"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69466.1"
                     /translation="MTIRYALTSLGKPLMAALAVAGGLTVSAQSYIQSTCDYAPLTTE
                     GTVICLGDDAVSSAIPLGFSFNFYDVPYTDCYVSSNGYLSFAAGLGSACCTGQILPNA
                     TYPASIFFGQEDLDPNSCIDGDISYYTTGAPGSQIFVLSFVDVPHYPGPEGTFPVTVQ
                     VQLYEGTNEVRIVTTQWNSDGGAATMGLNENGSNADPVSGRNSEVWSAFDECISFVPN
                     TEPTGGCMDAAAINYDPGADFDDGTCFYGYIASTCDYSPMATEGTIVCLGDDQVSTAI
                     DMGFDFPFYGTPHNQAYISSNGFVTFQPGMGSGCCTGQILPNATYQNTIFIGQEDLDP
                     NTCVDGEINYYTTGAPGSQVFVVNFIGVPHYPGPAGTFNITVQLQCYEATGEIRIVTT
                     EWNSDGGAATMGLNYNGTYADWVPGRNSSVWDAFDECYSWMPLTEPLPVCDAPGGLFA
                     DGITTDDAVLHWDAVDGAMNYNVILQNTATGLLKKKVFTTNMAVIENKLSPLTNYAFR
                     VKTGCGVDPENMSDPSPWYYFTTLGRTGEEQATVTLFPNPNNGVFTLNVAGYADNSFN
                     LNIYNTMGAIVYTKTVDITSDNQAVVVSLENVPGGMYQINLSNDQYQINYPVIVTK"
     gene            complement(10506..12038)
                     /locus_tag="PLL28_08835"
     CDS             complement(10506..12038)
                     /locus_tag="PLL28_08835"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNF69467.1"
                     /translation="MSRLLLHNAHVHTQDIQHPYATSILVDGERIIAVGDETLLLEAG
                     DIQKIDIGGRHIFPGLHDAHIHIWKVGNLLTNMLDLRGVGSIDEMQQRLSDYARSHPE
                     LTWIQARGFNEVIFPDKRIPNKLDIDAVISDRPVVITRTCAHQVVANSKALLLAGLDK
                     NMQPVPGGEIKYLASGELSGHFTETAIGLILKAVPKYTAAELRNMVLTAQDAFLKLGI
                     TAACDPAVDRDLLEVYKQMDADGELRMRINAIPIRVPDGSQKIYPNPQHYYSSHLVVN
                     TVKFFADGGLSGKTAALRLPYLNDTSKGVMRLEKEQFYQLALESQEAGFKIATHAIGD
                     AAVDLVLQVYDNLPLSNIEHRLEHVGFVDDNHLDIMYRRKVSAVMQPIFIRELGKNFI
                     VSLDKKRLDSIYPIHTIAAHGINVALSTDAPVVRSFDPRTNMLAAQTRETEEGIVIGA
                     SEILDHKQSTAMYTHGSSVAVGNVANIGTIAKGRYCDLVIHDFPDLSGSAEVWLSGSK
                     IR"
     gene            complement(12043..>12999)
                     /locus_tag="PLL28_08840"
     CDS             complement(12043..>12999)
                     /locus_tag="PLL28_08840"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNF69468.1"
                     /translation="NIARYATKRSAVICFEGGYHGRTLLTLSLTSKYALFKKGFGPFV
                     SDVVRLPAPNMYRKPQQFSEAEYIQFCIQQFDNAMIAQIDGDSVAAIIIEPVQGEAGF
                     IPMPKEFLQHLRNVADKHGIVLIFDEIQCGASRTGKFFACEHAGVIPDMICMAKSIGA
                     GMPISAVTGRASIMDAPHLGGVGGTYGGNPIACVAAIEAVKVLSSTEFLNRVQEVGNL
                     ISAQLQNWKEKYALIGDVRGIGAMQLVEFVSNRNERTPDPDVTLEIIRDAVAHGLLMI
                     RAGLYSNCIRLLPPVVMTDAQLQEGLAVLEAAIARAHNKRGL"
BASE COUNT         3915 a         2692 c         2769 g         3623 t
ORIGIN      
        1 gtaaaaacag acctaccatt gcctgcatct tattcttttt caaaaaattc tcgatataaa
       61 tggccctgtt tgggtcattg acgataggct ctgcataagc aaagaattgg tacttatcgg
      121 caatacttgg acgcccttcg tcacccgaag cctgcaattt ggcaggggtt ttgcccatag
      181 taacaaacag aaaggcagca gaaacaacac caacaagtat ggtatcgata gcgaatgccg
      241 aaatacgttt aacaggggat gcagtttgat agttgtgtgt cattgtaggt cgctttttgc
      301 gctgcaaaga taacatataa tcgccaagcc gttaacactt atcaacgtgt ctaacttcag
      361 gtttgctacg tgttggagcg gcaatttcgt aactttgcac ctccctgaag gatttgaagg
      421 atggaacata tcagaaactt ttgcatcatt gcccatatcg accacggtaa aagcaccctg
      481 gctgaccgct tattggaggt aactaagacc gtttcagagc gggaagcaca ggctcaggtg
      541 ctcgatgaca tggatattga gcgggagcgc ggaattacca tcaaaagcca cgccatccag
      601 atggatttcc gatatgaggg gaaacaatac gttctcaatc tgatcgacac tcccggacac
      661 gtagatttca gctatgaagt atcgcgaagc attgcggcat gtgaaggcgc cttgttgctt
      721 gttgatgcta ctcaaggtat ccaagcgcaa accatttcaa acctttacct ggcaattgaa
      781 cacgatctgg aaattattcc ggttgtgaat aagatcgata tggatggtgc catggtggat
      841 gaagtaaaag atcagattgt cgaccttatc ggttgtaaac gcgaagaaat tcttgaagcc
      901 agcggcaaaa ccggcgtagg tatcgatagc atttttgatg ccatcataca tcgcattcct
      961 tcgccaaaag gcaatgtgga tgctccatta caagcgctga ttttcgatag cgtgtttaat
     1021 agttatcggg gtgttgttgc ctactatcgg atttttcagg gaacaattaa aaagggcgat
     1081 aaaatcaaat tcgtcaacac ccaaaataat tacgaagcaa atgaagtagg aattctccga
     1141 ttacaatatg aaccacgttc cgaagttaag gcgggcgatg ttggctatgt gattacggga
     1201 attaaggagg cgaaagaaat taaggtaggt gatactatca cacttcgtga taacccttgc
     1261 agcaatgaag tggcaggatt taaagatgtg aaaccgatgg tgtttgcagg tatctaccct
     1321 atcgacaacg acgattttgc cgatttgcgc gactcattgg aaaaattaca actgaatgat
     1381 gcatcgcttg tttttgaacc cgaaagttca gtagcattgg gatttggatt ccgttgcgga
     1441 tttttaggtc tgttacatct tgaaattgtc caggaacgat tagagcgcga gtttgaccag
     1501 aatgtaatta ttaccgttcc aaacgtttct ttcgattgtt atacaactgc gggcgaaaag
     1561 atcgtggtac acaacccgac cgacatgcct gatccgggaa gtattgataa aattgaagag
     1621 ccttatattc gttcacagat cattacagcg cctgattata tcggtgctat tatgaaattg
     1681 tgtatggaca agcgcggtat attgaaaaat caggtatatc tgactaccaa tcgcgttgag
     1741 ctaacatggg agatgccttt agcggaaatt gtatttgatt tttacgataa attaaaatcc
     1801 atttcgcgcg gttatgccag ttttgactac gaattgattg attatcgtga gggcgattta
     1861 attaaacttg atattaaatt aaatggcgag caggtagatg ctttctcggc actcatccat
     1921 cgcgataagg cttatgattt tggcagcagg ctgtgtggta aattgaaaga tctcttgcct
     1981 aagcaacaat tcgtaattgc cattcaagct gccatcggtg cgaagatcat agcacgtgaa
     2041 accattagtg ccatgcgaaa agatgttacc gctaaatgtt atggtggtga tatttcacgt
     2101 aagcgcaagt tgttggagaa acagaagaaa ggtaaaaaac gcatgcgtca gataggcacg
     2161 gtagaagtac cgcaggaagc tttccttgca gtattgaaac tgaatgagtg atattcttgt
     2221 ttaatcgtca ctgcgatcgc atacaattaa attacagata gtgcattggt gcatttctgt
     2281 attcaaataa acaataaatt ctgcaatacc ggatggcaca gccaattatt ttagacggaa
     2341 agaaattatc gactgagatt caggatgaga tcagaaaaaa gatcgatata cgccgtactg
     2401 caggattaaa aattccacat ctcgcagccg tgcttgttgg taatgatggg gcgagtacta
     2461 cctatgtaaa cagcaaaatc cgattgtgta aatcggtggg tatgcaaagc acgcttattc
     2521 atttagatgc ttctacttct gaagaagaat tactacgtgt tgtacatgat ttgaatacaa
     2581 atcccgatat agatgggttt atcgtacaat tgccattgcc aaaacatatt caggagatgc
     2641 atattgttga agccattgac ccatcgaaag atgttgatgg tttccatccg gtgaatctcg
     2701 ggagaatgca attgggtttg cctgcattta tttcggcaac accggcaggc attatgacca
     2761 tgttaagcag atatcatatc gatactgcag gcaaacattg cgttgtgata ggtagaagca
     2821 ataatgttgg cacacctatg agcctattat tatcgcgaaa taccaatccg ggaaattgca
     2881 cggtaacctt atgtcacagt cgcacaaaaa atcttacgga aatttgtcgc agtgccgata
     2941 tcattattgc tgccctcggc aaaccgaagt ttgttacagg cgatatggta aaagaaggtg
     3001 ctgttgtaat tgacattggt atcagtcgcg tggatgccga caatgaaaaa ggatatctca
     3061 ttcttggtga tgttgatttt ccgaatgtag cgccaaaatg ttctgcgata tcgcctgttc
     3121 ccggaggtgt tggtttaatg acggtggtat cattaatcat gaataccatg caggcagcag
     3181 aatcgcgtga taaactataa ttatttttgt ttcatcaata atttcctcag cacatggaat
     3241 taccggtaat ggagcagttt tatacaattc agggtgaagg atttcatcag ggacgagctg
     3301 cctatttcat tcgtttggga ggttgtgatg taggttgtgt ctggtgtgat gttaaagaaa
     3361 gttgggatgt tgatgctcat ccaaaactga gtgtttcatc gctggtaaat aatgccgcag
     3421 cattcgcatc taaaattgtt gtaatcaccg gaggtgaacc cttgatgcat aatttatcag
     3481 cattgacaaa tgagttgcat gaaaagggtt taaataccca tttagaaaca agtggtgccc
     3541 atcccttatc aggcatttgg gattggatta cattttctcc gaagaaattt aaatcgccct
     3601 tagcatcagt agctgcacaa gccaatgaac tcaaggttgt gatcaatcat aaaagcgatc
     3661 ttgtatttgc cgagatgcat gcggctaccg taagtgaatc ttgtctatta tatcttcaac
     3721 cggaatggga aaagcgagat agtatgatgc cattgatcac tgagtatgtg cgcacaaatc
     3781 ctcaatggcg tatttctttg cagacgcata agtatttaca aataccataa aaaaaggctg
     3841 cccaaacgga cagcctttct tatgaagaac taatgaggtg attagtcagt aatcactacc
     3901 tgataattca ttacgccttt atcagttaag atatttacgt aatacattcc ggcagcggca
     3961 ttattgaggt ttacattttg agtaaatcct gtagcattca cattaaccgt attgttatat
     4021 actgtttgac cgagagcgct aacaatttga atatttactt gttgaccttc ataaccgttc
     4081 aatgcaatat caaaagttcc ggtattaggg ttagggaata acactacatt agtattttca
     4141 tctccaagac gagcaggagt agtgaaatag tagtgataag aaatatcaga agccatacct
     4201 aaaccggtac aaattgaacg cacgtggaat gcataatcag ttcccggagt aagacctgtt
     4261 acagtccaat ctgtagcacc accgagtact tttttcttca acaccaatgt ttcatcagtt
     4321 ttatacactg acaaatgata accaccggag ttcagtgatg gatcccagaa caagtgtgca
     4381 gaagtacctg taattgcatc agcaccaaga cccactggag caggacaatc agttaccact
     4441 tcgcaagaca ggtagctgaa gatattagcg cgaagatttg ctgcattagg atcaggatcg
     4501 tgccagttgt cggtagtcat accaccaaac attacaatac caccgcccca agttttttca
     4561 gcgagaacaa catttgaagg agcagcagca tcaaccataa cattggtaag acctgttcct
     4621 gtaatgcgtg catgtccgaa tgaagttccg gtataggtag ttcctgcagg tgtatatgga
     4681 ccagagaaaa tcgggtgacc gccaacaccg tttgcagtgc ttgtgaaata agaataaacc
     4741 aatgaagttc caccaaagcc gaagctcatg ccatcgcctt catttggagc agcgtttaat
     4801 aataatttac cgcctgagct aacccaagat tcaataactg ccatatttgc agtgagatat
     4861 gtttcaagtt cattggcatg accatcagaa ccttccataa atacaaagca attatcaggg
     4921 gtgaacacag tagctgcagg agcagtttca taataatttt tattccatcc ggtaccttca
     4981 gcgccaaaaa cggtggtcat tgcagtacta ttattggtag taaaccaagg ttcaccgccg
     5041 gtggtgtttg aaagcatata gatcgctcct gttccggtaa cggcaccggt atgacaaact
     5101 tcttcggtat agctatcgtt gcccggatct tcatcacctg ctaatgtagt ccatgcagtg
     5161 atattataac atccttctgc gctgaaatcc cagccttcag gtgcagtcca tgaaccggta
     5221 gaaaaaggtg caatggtacc accataaaat ccggctgtta ccaaaccgcc gtcaacttga
     5281 taagatacgg tgaatcctga agcaggttct gaaccataat tgcgaacggt aacgataaca
     5341 tcttccaatc ctaaaccggg accggttgtt gggttggtga tatcaattac accaacatca
     5401 ttcatagcgc cacccaaaag gttaacagta taatcttctg tttcaccgta tgtagctaag
     5461 gcacaaggat caagaggtgt aactaaacct gaaggataaa tgcatcttac gcgcattctt
     5521 gtagttccgg acattgctgt tacaggtacc gtaaatgaaa atgatcctgt agctgatgca
     5581 ctcaaactca ggttacccaa aacttcagat gtttcaaacg tgttgtcatg atcatagtcg
     5641 atccaggcag tgtaagtctg cgaccatgaa gggttgttat tacatgtcat ggtgtaagca
     5701 tcgccgatat tcaggtctgt agacaggaaa gtatagtcgg aataaccaac acctgttatt
     5761 gcagcacctg aaaatgtgtt ggcgatagtg ccaactgtta cgccattgat gtaatctcct
     5821 tcggctgttc ctgtagtgta agtcggcgta caatactgcg ctttagcagc gattgaagag
     5881 cctattactg ctgccagtac taaacctgtt cgtaaatgtt taagcatgtt tttcatatga
     5941 gtcgtttaag tgaaatgaat cattgaaaat aatgattaaa aatcaggcct ttgcatactt
     6001 agatcatgtt gcaaattttg cttttgctca tttcattgga aatgattaca aactatcaca
     6061 taattaactg attttcaatt atttacatat catattcact ttacttatat aaaagttcaa
     6121 tatgataatt gtattgaaaa aataaataac tgtttggtca tttatggcat aaaaaaaggc
     6181 ggtcgaatcg accgcctttt caacttcgga aacccgaatt atttagttac tacgatagcg
     6241 tagttgattt ggtattgagc gttgctcaga ttcacctgat acatacctgc agggatgtta
     6301 tcaagagcga tctgttctgc atagttagca cctgttacgt caacagtttt agtgtaaacg
     6361 atttgaccgg tagcgctaac aacgttgagg ttaaagctat tgccttcata acctgctaca
     6421 ttgatagtga acgtaccgtt gttagggttc ggatacaaag aaactgcggc ttcattcaca
     6481 ccttcgcgac cgagggtagt gaaataatac cattcagaag ccattgaaac gatatcagga
     6541 tctacaccgc aaccagtttt cacgcggaaa gcatagttag tgagtggaga aagtttattt
     6601 tctatcacag ccatattggt tgcgaacact ttctttttca acaaaccagt agcggtattt
     6661 tgaaggataa cgttataatt gagggcacca tcaacaacat cccaatgcaa tactgcatcg
     6721 tcagtagtaa tgccatcagc atataaacct cccggaggca agcagataac gaattccata
     6781 cagctcatat aagcgataat gtttgcgcgc aggtttgcag cgtttgtgag tggtgaatgg
     6841 aagttgttag tagtcatacc accaaacatc acgcgaccat cgccccaagt tttttctgcc
     6901 aatacaactg cagatggatt gaagagatca accattacgt tgctaagatc aacaccgctc
     6961 acgattgcgt gaccgaatga agtacctgta tagtcagttc ctgcaggagt gaaaggaccg
     7021 ttgaagattg gatgcaatgc atcaacagca gttgcatttg aagtgtagta tgaataagta
     7081 agtgtagttc caccaaagcc aaagctcatg ccatcacctt cattaggagc agagttgagc
     7141 aacagtttac cacctgaaga cacccagttt tcgatagtag acatgttagc agtgaggaaa
     7201 gtttcaagtt cattagcttg agcatcagaa ccatcgataa atacgaagca gttgtcagag
     7261 ttgaagattg acagtggatc gagtgtttcg aagaaagcgc gattccatcc tgttccttca
     7321 gcgccgaata ctgtagtcat agcagttgag ttggttgtag taaaccaagg ctcaccgcct
     7381 gtagagttag agtagatgta ccaagcacct gttcctgtaa taggaccgag gttacaaact
     7441 gtttcggtat agtcatcatt atctgcaatt tcatcgagtt cataatcggt ccatgcagtg
     7501 atgtcgtagc aaccatcagc agtaaaatcc caagtatcac caaaagtgta agatgcagtt
     7561 gagtaggcat cgattgttcc gccgtaagta gcgcctgtaa ccagaccgcc atctacctgg
     7621 aagtaaacgt taaatcctga agcaggttca gaaccgtagt tacggatagt gatggtaacg
     7681 ttttcaaagc caagatcaac agctgaaatt gggttagtga tgtcaattac accaacgtcg
     7741 ttagcagcac cgccaacaat gttaatggtg taatcttcag cttcgccata ggttgcagat
     7801 aaacaaggat cctgaggaat tgcaagaccg cttgggtaga tacaacgaac gcgcatgcga
     7861 gttgaaccac ccggagtgcc aacaggtaca gtaaatgtaa ctgttccgga agcaccggct
     7921 gaaagtccta caccacccaa tacctctgtg gtttcaaata cttcatcctg atcataatcg
     7981 atccaggctg tataggtttg agaccatgaa gggttgttat caagggtcat tgtgtaggtt
     8041 gaaccaacgg taagatcaga agataatgcc gtgtagtctg aataacccac tccggtaatt
     8101 gcggcaccgg aacccgtgtt ggcaatcgcg ccaactgtta ctccattgat atagtcgcca
     8161 tcagcagttc cggtagtata gacaggtgtg caatattgtg cactggctaa tccggtagta
     8221 acgacaacca cacctagagt aagccatgtt tttgcgtaag aaagcatgtt catcataagt
     8281 tgtgatttag gttaaaaaag aatgactgca atatacgaaa gaatttgaaa atgacaagag
     8341 attatcatgt acaaattgct gatgacctct atgcaatgac agataattaa taaatgtcat
     8401 taattgttga aattattaca atctctctcg cagttgcaag ttcaattatt tgatgtacta
     8461 catgtaaacc acgttgcatt tcaaataaaa aagggtgccg aaatcgacac ccttttacaa
     8521 aataataagt taaatcaatt atttagtaac aatcaccgga tagttgatct ggtattgatc
     8581 attgctcagg ttgatttgat acataccacc aggaacattt tccaatgata caaccactgc
     8641 ttgattatca gaagtgatat caacagtttt agtataaaca attgcaccca ttgtattata
     8701 gatattcaaa ttgaaactat tgtcggcata accggcaaca ttcaaagtga aaacaccatt
     8761 attcgggtta gggaacaaag taacggtagc ttgttcttcg cctgttcttc cgagagtagt
     8821 gaaataatac caaggtgaag gatctgacat attttcagga tctacaccgc aaccagtttt
     8881 cacgcggaaa gcatagttag tgagtggaga aagtttattt tcaatcacag ccatattggt
     8941 agtgaatact ttctttttca ataatcctgt tgcagtattt tggagaatta cattatagtt
     9001 cattgcacca tcaactgcgt cccaatgtaa cacagcatcg tctgtagtaa ttccgtcggc
     9061 aaaaagacct cccggagcat cacaaacagg cagtggttct gtcaaaggca tccaagaata
     9121 acattcgtcg aatgcatccc aaacagagga gttgcgacca ggtacccaat cggcataagt
     9181 gccattgtag ttaagaccca tggtagcagc gccaccatcg ctattccatt ctgtagttac
     9241 aatgcgaatc tcgcctgtag cttcatagca ctgcaattga acagtgatat tgaaagtacc
     9301 tgcaggtccg ggatagtgag gaacgccgat gaagttcaca acgaatactt gtgaacccgg
     9361 agcacctgta gtataatagt tgatctcgcc atctacacaa gtattaggat cgaggtcttc
     9421 ctgaccaata aagatggtat tttggtaagt agcatttggc aatatttggc ctgtgcaaca
     9481 tccgcttccc ataccgggct ggaatgtaac aaaaccatta gagctgatat atgcttgatt
     9541 atgtggtgtg ccatagaatg ggaaatcgaa gcccatatca attgctgtgc tcacttgatc
     9601 atcgccaaga catacgatag ttccttcagt tgccattggt gagtaatcgc aagtagaagc
     9661 gatataacca tagaaacaag taccatcatc gaaatcggca cccggatcgt agttgatagc
     9721 agcggcatcc atacatccac ctgtaggttc ggtattaggt acgaaagaaa tacactcatc
     9781 gaatgcagac catacttcac tgtttcttcc tgaaaccgga tcagcatttg aaccattctc
     9841 atttaaaccc atagtagcag caccaccatc ggagttccac tgagtggtta cgatgcgtac
     9901 ttcatttgta ccttcataca attgtacttg tacagttaca gggaatgttc cttccggacc
     9961 gggatagtgc ggaacatcta caaagctcaa cacaaaaatt tgtgaaccgg gagcacctgt
    10021 agtgtaatat gaaatatctc catcgataca agagtttgga tccaagtctt cctgtccgaa
    10081 gaaaattgat gccggataag ttgcatttgg aaggatttgt cctgtacaac atgcgcttcc
    10141 gagacctgct gcaaatgaaa ggtatccatt tgagctcaca tagcaatcag tgtatggaac
    10201 atcatagaaa ttgaaggaga atcccagagg gatggcagag ctaactgcgt catcacccag
    10261 acagatcaca gttccttcag ttgtcaaagg ggcgtaatca cacgtactct gtatgtacga
    10321 ttgcgcagat acggtcaaac cacctgcaac tgccaaagca gccatcagag gctttccgag
    10381 agaagttaat gcgtaacgaa tagtcatatt tagagattta agctacaagt atagcgtttt
    10441 ttttgggtaa atggggctat taagtgttaa aaatcaccct catctgcatg ataaacttta
    10501 aaaattcatc taatcttgga accactgagc catacttctg ctgatcctga aagatccgga
    10561 aagtcgtgaa taaccaaatc gcaataccga cctttagcaa tagtgccgat gtttgcaaca
    10621 ttgcctaccg caacagatga gccatgtgta tacatagctg tagattgttt gtggtcaagg
    10681 atttcagagg ctcctattac tattccttct tccgtttctc gcgtctgagc tgcaagcata
    10741 tttgttctcg gatcaaagga tcgaacaaca ggcgcatcag tagataatgc cacattgatg
    10801 ccatgtgctg ctatggtatg aatagggtaa atgctatcca accttttttt atcgagtgat
    10861 actatgaaat tcttacccag ctcgcgaatg aagattggtt gcatcacagc cgataccttc
    10921 cggcggtaca taatgtccag atgattatca tccacaaatc caacatgttc aagcctgtgt
    10981 tcaatatttg acaaaggcaa attatcatac acttgaagca ccaaatcaac agcagcatcg
    11041 ccaatagcat gagttgcaat tttaaatcct gcttcctgcg attcaagtgc taattgatag
    11101 aattgttctt tttccaatcg cattacacct ttcgaagtat cattcaagta tggtaggcgc
    11161 agtgccgctg ttttcccgct cagtccgcca tcagcaaaaa actttacggt atttacaact
    11221 aaatgtgatg aataataatg ttgagggttc ggatatattt tttgagaacc atccggtacg
    11281 cgaatgggaa tggcatttat tctcatgcgc aattcgccat cggcatccat ttgtttatat
    11341 acctctaaca aatcgcggtc gactgcagga tcacaggcag cggtaattcc caacttcaga
    11401 aaagcatcct gagctgttaa taccatgttg cgcaattcag cagcagtata ttttggaaca
    11461 gcctttaaaa tcaagccgat agccgtttcg gtaaaatgac cgcttaattc acctgaagca
    11521 agatatttta tttcgcctcc gggaacaggt tgcatatttt tatcaagacc tgccagcaaa
    11581 agcgctttag agtttgcaac aacctgatgc gcgcaggtgc gtgtgatcac taccggtcgg
    11641 tcggaaatca ccgcatcaat atcaagttta ttcggaatgc gtttatcggg aaatattact
    11701 tcattaaagc cgcgtgcttg aatccatgta agttccggat gtgagcgagc gtaatcactg
    11761 agtcgctgtt gcatttcatc aatactgcca acgccgcgca aatcgagcat gttagtcagc
    11821 agattgccca ctttccatat atgaatatgg gcatcgtgta atccgggaaa aatatgtcga
    11881 ccaccaatat caattttttg aatatcgcct gcttccagca ataaagtctc atcgccaacg
    11941 gcaataattc tttcaccatc tacgagaatg gaagttgcat aaggatgttg aatatcctgt
    12001 gtatgcacat gcgcattatg taacaggagg cgactcatat atttacaatc cgcgtttgtt
    12061 atgtgcacgt gcgatggctg cttcgagtac ggcaagtcct tcttgcaatt gtgcatcagt
    12121 cataactaca ggaggaagca atcgaataca atttgaatat aatccggcgc gaatcattaa
    12181 taatccatgt gcaacagcgt cgcgaataat ttctaaggtt acatcaggat caggggtgcg
    12241 ttcatttcta ttgctcacaa attcgacaag ttgcatagca ccgattccgc ggacatcacc
    12301 tatcaatgca tacttttctt tccaattttg aagctgtgct gaaattaaat taccaacttc
    12361 ctgcacgcga tttaaaaatt cagttgatga taacactttc actgcttcga tagcagcgac
    12421 acatgcaatg ggatttcctc cataggtacc tcctactcca ccaagatgcg gtgcgtccat
    12481 gatcgaggct cttcctgtaa ctgcgctgat cggcataccg gcacctattg atttcgccat
    12541 acatatcata tccggaatta cacctgcatg ttcacatgca aagaatttac cggttcgcga
    12601 agcaccacat tgtatttcat caaatattaa tacaatgcca tgtttatcgg caacatttct
    12661 gagatgttga agaaattctt tcggcattgg gataaaccct gcttcaccct gaaccggctc
    12721 aataataatt gctgcaacgc tgtcgccatc aatttgagca atcattgcat tatcaaattg
    12781 ttgaatacaa aattgaatat actccgcttc cgaaaattgt tgtggcttgc gatacatatt
    12841 tggcgcagga agacgcacga catcacttac aaacggacca aagccttttt taaataaagc
    12901 atatttactg gtgagcgata atgtcaacaa agtgcgacca tgatatcccc cctcaaaaca
    12961 aattacggcg cttctttttg tggcatagcg cgcaatatt
//
LOCUS       DAOQHY010000094        12975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2353_length_12975_cov_5.748994, whole genome shotgun sequence.
ACCESSION   DAOQHY010000094 DAOQHY010000000
VERSION     DAOQHY010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12975
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2353_length_12975_cov_5.748994"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1839
                     /locus_tag="PLL28_08845"
     CDS             <1..1839
                     /locus_tag="PLL28_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNF69469.1"
                     /translation="KVSRVLFDKRIIMLDLAALVAGTKYRGQFEERMKAIMNELEKNR
                     DVILFIDEIHTIVGAGGATGSLDASNIFKPALARGELQCIGASTLDEYRQYIEKDGAL
                     DRRFQKVLVDPPTAEETMEILGNIKSKYEEFHNVNYSDAAIKACVTLSTRYITDRYLP
                     DKAIDVLDEVGARVHLKNIHVPDEIVKLEGEIEAIKDEKNKVVKSQRYEEAAKLRDTE
                     KRLLEDLDRAKTKWEEHVKSERYPVTEDDIAEVVAMMTGIPTKRIAQSEGTKLVGMAD
                     ELKRGVIGQDDAIVKITRAIQRNRVGLKDPKKPIGSFIFLGPTGVGKTELAKVLSEYL
                     FDSKDALIRIDMSEYMEKFSVSRLIGAPPGYVGYEEGGQLTEKVRRKPYSVILLDEIE
                     KAHPDIFNILLQVLDDGILTDGLGRKVDFKNTLIIMTSNIGARQLKDFGTGVGFSTAA
                     KQLAQEENTKSVIETALKRTFSPEFLNRIDDVVIFNSLSREDIFRIIDISLRDVTKRL
                     ETLNYKLQLEESAKEFLSERGYDPQFGARPLHRAIQKYLEDPLAESILGGKFKEGDTL
                     LVEFDKDLGGLVINPAGEKEAEKEAEKEKKKGKGKDKKEEREEEKA"
     gene            1987..3534
                     /locus_tag="PLL28_08850"
     CDS             1987..3534
                     /locus_tag="PLL28_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69470.1"
                     /translation="MKFAILYALMLMSTIASAQADFEWYASYNNVTFSSFGYPKDIVL
                     DNEGNVVACGYTAINDTSQSLMTIIKLSPAGDQLWNFNMTLSDEYPALVTALDVNSNN
                     EILFTGETGDTMIVGKLNPDGLLQWIEKYAINKYWNHGRDIHVTATDEILVSGDCSND
                     TVVLLKYTNDGDLLWERFRQRQFYFGFVYTAFDSESNTYQCYEVPGTDEYTDFEVCKY
                     NAEGDLMWAHAYSNFSLTAFARDFAISDDGCAYVLNRAYGNSQLYELVKIDSVGDLAW
                     SAYLDNFPDQNYFTYATDIAVDKDKNVMVIGEQYDSDLLVDYKNIVKFNDDGAYVWQK
                     GVDYIANTVSYGSDIFTDNYGNIYSMFNYGDTIPWSSTYMMINKRGPSGDLKWSATFS
                     ENCYPEQLLVNEDEVYALITGPGDNLYDAIGVIKYSQVVTMQSQAPQTIAMQAYPNPA
                     IDYIQFMANANVELMHAETYNIAGELVDVNIKLNTPCDVGMLPAGYYTSRCITSLGTA
                     TIAWVKQ"
     gene            complement(3563..4099)
                     /locus_tag="PLL28_08855"
     CDS             complement(3563..4099)
                     /locus_tag="PLL28_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69471.1"
                     /translation="MNRKLLFLLLSSILVFSASAQQTQKLRKHYYILIDDTTFVNAMP
                     EQGATLKAYFKNDTIYKIETWFGFNFGEVTRDYYYWDDTLSLVNETQKLYNAEATPKI
                     NPDTIKTSYAGRYIFQHNKLTGISQKGSYSISDTPQTKEDTQAALLMLSDKYRVLAYE
                     KRKAKKNRVKIKSNELVK"
     gene            4154..4765
                     /locus_tag="PLL28_08860"
     CDS             4154..4765
                     /locus_tag="PLL28_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNF69472.1"
                     /translation="MAGLREKLLAPDNRVRRNPMVIVVNMLLASIVVLFLALTYGYAL
                     RMGSNWLEFRLPKVFWLSTICIMLVSFFLRRTIKYYDTDRTARLRRAMALATIMATAF
                     VVCQLIGWGELGKAGILMDTSTSASYLYLLSGLHLLHVGVGIIILLVGCVRLFTNTST
                     PVKALLYYADPVRRDRLKLLNHYWHTIDFLWLFLFLVILYNQT"
     gene            complement(4839..5264)
                     /locus_tag="PLL28_08865"
     CDS             complement(4839..5264)
                     /locus_tag="PLL28_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69473.1"
                     /translation="MKKLLMTLSFVLAVTAGQVFAQSAPSGDANKEAKKTEVKKVAPE
                     KRQAITPAQAKKKAAMNGKMNAKPAKARVKAHQDEIQAHDASKAEVKAHAQDAKANKT
                     EADKKEAVKAHKDEIKAHADSKEEAKAKLQRKHDKKKNE"
     gene            5437..5513
                     /locus_tag="PLL28_08870"
     tRNA            5437..5513
                     /locus_tag="PLL28_08870"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5471..5473,aa:Pro,seq:ggg)
     gene            5719..7752
                     /locus_tag="PLL28_08875"
     CDS             5719..7752
                     /locus_tag="PLL28_08875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HNF69474.1"
                     /translation="MQFVYPAFLFALATLAIPVVIHLFNFRRFKTIYFSNVRFLRNVQ
                     EETATKNKLKHLLVLLTRCLALAALVFAFAQPFIPAKKSGNNSSDRAVSIYIDNSFSM
                     EAEVNDERLIDIAKRKAEEIVNGFTIDDRFQLITNDLEAKHQRLVSKDEMIRFIAEVQ
                     PSPVVRPLQEISDRQRDALHREGDKAEKISYLLSDFQKNAGDIDNDTSIIVNFVPLLQ
                     RAQKNVAIDSMWFTTPVPVLNQPISICIRLHNYGDGDVENVPVTLRLNEQIKAIADIN
                     INAKGDVVDTMTFTLNSGGWYSGEISIKDYPVTFDDMLYFSFKPVEQIPVLAINGGSE
                     NIFLKGFFGNNNLFQLKNNPASQIAFSELPTYDLIILNEVPSISGGLADALQQHLARG
                     GNIMVWPSLTMDVASYQAFLLANNAAVYGALINRPRPVSEVDTKNPVFADVFEKIPRN
                     LAMPTAMKSYALQTSTGTREEMLLRFTDRQAMMARYASGDGYIYLSAVPLDRTVTDLP
                     VQAGLFAPIMYKVAVSNARRLPAYANIGEMGWINLPGIQLPGDATIKVESKGNEFIPE
                     IRRTGNSVAVNLSSYVQQAGWYTIVPDKQMVNSDQLLALNYNRSESDMSFADASALKE
                     LYAASNQEIISDIDRNFTGMVTQMATGTPLWKYCIIFVLVFLAAEIAIIRLMP"
     gene            7765..9030
                     /locus_tag="PLL28_08880"
     CDS             7765..9030
                     /locus_tag="PLL28_08880"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNF69475.1"
                     /translation="MHCILQGATIIDPASQHHRSTVSIRIQNGVITEIASSIADTGDA
                     QLIDLKGNFVSPGWMDMLAAFGDPGHEYRETIQTGAAAAAKGGFTALGLMPNTQPALH
                     GKAEIEYIKNHAAGQLTHIFPYGAVTHNREGKDLTEIYDMHAAGAMAFTDADSAIRDA
                     GILLRSLQYVKPFNGVIINIPNDHKIVGNANVNEGAMSVQLGMYGIPDILEELMVIRD
                     IKLAEYTQSHLHIGIVSSAKVLPHIRAAKEKGLSVTAGVAAYQLYFDETELHDYNAHY
                     KVNPPLRTAADKQALREAVADGTIDVVCSYHLPHDNDSKDVEFEYAAFGMASLEVVFG
                     AMRSALPNISPEQIVNILAAKPRNILNIAQPGIAVGNTADITMFDAETTWTLQQSDLR
                     SKASNNPFVGKTLKGKVLGVINKGKISIA"
     gene            9053..9580
                     /locus_tag="PLL28_08885"
     CDS             9053..9580
                     /locus_tag="PLL28_08885"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HNF69476.1"
                     /translation="MDVQPIERPSRKGLILRNGLIFVALQIAIMLVTHFRRDSISDQN
                     MRIAVGMYLLLLAAIVVVQLDARMRVYSGRMDFGQAFSTGMIFVLFVTALFTIFILVF
                     YLTIGKELLAQMITIAENSLRANGTAEADIPERMEFSRKIFTAPGMSIITLIGYLFFG
                     TIMSLIGALFTQRTN"
     gene            9580..10536
                     /locus_tag="PLL28_08890"
     CDS             9580..10536
                     /locus_tag="PLL28_08890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNF69477.1"
                     /translation="MQISVIIPLLNEEESLPELSQWISKVMQENNFSWEVIFVDDGSR
                     DKSWQVVQQLHADNASFKGIKFRRNYGKSAALNEGFAMAQGDVVITMDADLQDSPDEI
                     PGLYKMIADEGFDMVSGWKKKRHDPIGKTIPSKFFNWTTARITGIKLHDFNCGLKAYK
                     RDVVKSIEVYGEMHRYIPQIAKTAGFYKIGEKVVEHRARKYGYSKFGWERFMKGFLDL
                     TSIMFVSKFGKKPMHLFGTFGFLSFFTGGIITIWLIIDKIIKMNTGEKFRQVTDQPLF
                     YIALVAMIVGVQLFVGGFLGELISRNSPDRNQYQIDQRIGGV"
     gene            10533..11651
                     /locus_tag="PLL28_08895"
     CDS             10533..11651
                     /locus_tag="PLL28_08895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF69478.1"
                     /translation="MKILLIGTAWPYRGGLASFNERLIQEFITEGDDASIITFTLQYP
                     GFLFPGKTQYSSSPPPGIPITRKVNAVNPFNWWRIGKAIAKEKPDIVIIKYWMPFMAP
                     CFGAIARHIKKNKHSSIICIADNIIPHERKFYDKPFTKYFISAMDGFVLMSKSVMEDL
                     AQFDRKKIRALNPHPLFDNFGDGVSKTEALARLHLELNRRYILFFGFIRDYKGLDILL
                     EAMHAIPEDVHLIIAGEYYTDSKPYDALINQYNLSDRVHAFNRFIPDEEVKYFFCAAD
                     LVVQPYKHATQSGVTQIAYHFEVPMVVTNTGGLAEMVLDGQVGYVTPAEPLAIAQAVS
                     KFYVEGKSEEFKRNLREEKSRFSWSRMTSTIRRLYQKG"
     gene            complement(11662..>12975)
                     /locus_tag="PLL28_08900"
     CDS             complement(11662..>12975)
                     /locus_tag="PLL28_08900"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNF69479.1"
                     /translation="IQPQLKRFDLTMIVVSLVIGVGIFRTPAMVAQSAGKEWIFYAAW
                     ITGGIISIFGALTFAEIGARLPSAGGYYRIFSTAYHPIYAFMLNWSQVIINAGSSAGV
                     AIIGAEYLTPVLFGNTAEDYVVIDTAAIVIILILFGINYAGIKMGARAQNVLSLLKIV
                     MIITCCFAIFSGQANADIPVVIDEDWNFINAFGIALISIFFTYGGYQQTVNFGADVKD
                     PQKNIPLAIFFGVGIVIVLYLLINLAYVNVLGFEQMKNTPLIAKDLAEALFGNTGAVI
                     TSIILFTSVLGFLNTSMMSNPRVYYAMAEDKVLPQIFSKVNHKTQVQEFGLSFFVAVM
                     IASVFLLGNFENIVTYVLFIDSISLASAAGALFIFRHRSPKDYSGFKMKGYPYIPAVF
                     MLVLLFVTYSTFMDDATSAQYGFLIFLMGLPLFFLITFFSNKKQQ"
BASE COUNT         3883 a         2543 c         2811 g         3738 t
ORIGIN      
        1 aaagtgtcaa gggttctgtt cgacaagcgt atcattatgc tcgacctcgc tgcgttggtg
       61 gcaggaacaa aataccgcgg acaattcgag gagcgcatga aggcgatcat gaatgaactc
      121 gaaaaaaatc gcgacgtaat tctcttcatc gacgagattc atactatcgt tggtgcaggc
      181 ggtgcaacag ggtctcttga tgcatccaat atctttaaac cggccctcgc cagaggtgaa
      241 ctccaatgta ttggtgcttc taccctcgac gagtatcgtc aatacataga aaaagatggc
      301 gccctcgatc gccgcttcca aaaagtattg gttgatccgc caactgctga agaaacaatg
      361 gaaattcttg gcaatatcaa atcaaaatat gaagaattcc ataatgtgaa ttattcagat
      421 gctgccatta aagcctgtgt aacactcagt acacgttata tcactgacag atatctaccg
      481 gacaaagcta tcgatgtact ggatgaagtt ggtgcacgtg ttcacctcaa aaatattcat
      541 gttcccgatg agatcgtgaa actggaaggt gaaattgaag caataaaaga tgaaaaaaat
      601 aaggttgtta aaagtcagcg ttatgaggaa gcagcaaaat tgcgcgacac cgaaaaacgc
      661 ttactagaag acctcgatcg ggcaaaaacg aaatgggaag agcacgtaaa aagtgaacgc
      721 tatcctgtta ctgaagacga tatcgctgaa gtggttgcca tgatgaccgg aattcctacc
      781 aaacgcatcg cgcaaagcga aggcactaaa ctggttggta tggctgatga gctgaaacgc
      841 ggtgttatcg gacaagatga tgcaatcgta aaaatcacac gcgctattca acgcaataga
      901 gtgggactga aagatcctaa aaaaccaatt ggttcattca ttttcctcgg acctactggt
      961 gttggtaaaa cagaattagc gaaagtgctt agtgaatatt tattcgactc taaagatgca
     1021 ctcatccgca tcgatatgag tgaatacatg gagaaattca gcgtaagccg attaataggc
     1081 gcgcctccgg gatatgttgg atatgaagaa ggtggtcagc ttactgaaaa agttcgtcgc
     1141 aaaccatatt ctgtaatctt gctcgatgaa atagaaaaag cgcatccgga tatttttaat
     1201 attttattgc aagtactaga tgatggtatt cttaccgatg gattgggaag aaaagtggat
     1261 ttcaaaaata cactcatcat catgacttcg aatatcgggg cacgtcagct aaaagatttt
     1321 ggtacaggtg ttggattttc tactgcagca aaacaattgg cgcaggaaga aaataccaag
     1381 agtgtaattg aaacggcctt aaagcgcaca ttctctcctg agtttctaaa caggattgat
     1441 gatgtggtga tattcaattc actcagccgt gaagacatct tccgaattat tgatatcagc
     1501 ttgcgcgatg taactaaacg tcttgaaacg ctgaattata aattgcaatt ggaagaaagt
     1561 gcaaaagaat tcttgagcga acgcggatat gaccctcagt ttggcgcaag acctttgcat
     1621 cgcgcaattc agaaatatct cgaagaccca ctggcagaaa gtatccttgg cggaaaattc
     1681 aaggaaggcg ataccctgct cgtggaattt gataaagatt tgggcggatt agtgatcaac
     1741 cctgccggcg aaaaggaagc tgaaaaagaa gctgagaagg aaaagaaaaa aggcaaaggc
     1801 aaggataaaa aagaggagag ggaagaggaa aaagcctaat cttcaacata cttaatcaaa
     1861 ggccgtatcg agagatgcgg tctttttttt gtcaattcct tgtggcctta ttttacatgg
     1921 ttgtgataga aatattgtga taaaactgag caataacgta tatttgaata tatgtattcc
     1981 ccgcaaatga aattcgcaat actgtatgct ttaatgttga tgtcaactat tgcctcagca
     2041 caggcagact ttgaatggta tgcttcttat aataatgtta ccttttcttc tttcgggtat
     2101 ccaaaggata ttgtattaga caatgaaggc aatgttgttg cctgtgggta tactgctatc
     2161 aatgatactt cacaaagttt gatgactatt attaagttat cgcctgcagg tgatcaatta
     2221 tggaatttca atatgacctt gagtgacgaa taccccgcac tggtaacagc actcgatgtc
     2281 aatagcaata atgaaattct gtttaccggc gaaaccggag ataccatgat cgtcgggaaa
     2341 cttaaccctg atggcttatt gcaatggatt gaaaagtatg ccattaacaa atactggaat
     2401 catggtagag atattcatgt aactgcaaca gacgaaattc ttgttagcgg cgattgctct
     2461 aacgacacgg ttgtgctgct gaaatataca aatgacggcg atctgctatg ggaacgtttc
     2521 aggcaaaggc aattctattt tggattcgta tatacggcat ttgacagcga gtcaaatact
     2581 taccagtgct acgaagtgcc cggaactgat gaatatacag atttcgaagt ctgtaaatat
     2641 aatgcggaag gcgatttaat gtgggcgcat gcctattcta atttctcgct taccgcattt
     2701 gccagagatt ttgccatcag tgacgacggg tgtgcttatg tgctcaatag agcctatgga
     2761 aattcgcagt tatatgagtt agtaaaaatt gattcggttg gcgatctcgc ttggtcagca
     2821 tatctcgata atttcccgga tcaaaattac tttacctatg caactgatat tgctgtagac
     2881 aaggataaaa atgtaatggt aatcggcgag caatatgaca gtgatttgtt agtggattat
     2941 aaaaatatcg taaaattcaa cgatgacggc gcttatgttt ggcaaaaggg ggttgattat
     3001 atcgcaaata cagttagtta tggtagcgat attttcacag ataactacgg aaacatttat
     3061 tcaatgttta attatggtga taccatccca tggtcgtcaa cctatatgat gataaacaaa
     3121 cgcggtcctt ccggagatct gaaatggagt gctacattca gtgaaaactg ttatcctgag
     3181 caactgttgg tgaatgagga tgaagtgtat gccctcatca ccggtcccgg cgacaattta
     3241 tacgatgcta ttggtgtgat taaatattcg caagtggtaa ccatgcaatc gcaagcgccg
     3301 caaacaattg ccatgcaggc atacccaaat cctgctattg attacattca atttatggca
     3361 aatgcgaatg ttgaattaat gcatgctgaa acctacaata tagcagggga gcttgttgat
     3421 gtaaatatta aattgaatac gccttgcgat gtgggaatgt tgcctgccgg atattatact
     3481 tcaagatgta ttacatcact cggcacagcg accatagcct gggttaaaca ataagcagat
     3541 gtaaagtagt ttcgcgaatg gcttatttca ccaattcatt ggatttaatc ttcaccctgt
     3601 tttttttcgc cttgcgtttt tcataagcca acaccctgta tttatccgac aacattaata
     3661 aagcagcttg ggtatcttcc ttggtttgcg gtgtgtcgga aatactgtaa ctgccttttt
     3721 gcgatatacc ggtgagttta ttgtgttgaa aaatataacg acctgcgtat gaagttttaa
     3781 tggtatccgg atttatttta ggtgttgcct cggcgttgta taatttttgt gtttcattca
     3841 ccagcgaaag agtatcatcc caataataat aatctctggt aacttcaccg aaattaaatc
     3901 caaaccaagt ttcaattttg taaatggtgt cgtttttgaa ataggccttt aacgttgcgc
     3961 cttgctccgg catcgcattt acaaaggtgg tatcgtcgat caatatataa taatgcttgc
     4021 gcagcttttg agtttgttga gcggaagcag aaaataccag gatgcttgac agcagtaaaa
     4081 agaggagttt tcggttcata atagtgcaaa caaagttaaa tcaataattg ttacgaagat
     4141 gtaaatttgt ccgatggctg gcctcagaga gaaacttctg gctcccgaca atagggtgcg
     4201 gcgcaatcct atggtcatag tagtgaatat gcttttggcc agtattgttg tgctgtttct
     4261 ggcacttacc tacggctatg cccttagaat gggtagtaat tggctggaat tccgacttcc
     4321 caaagtgttt tggcttagca caatttgcat tatgttggta agcttttttc ttagacggac
     4381 aatcaaatat tacgacaccg atcgcacggc ccgcctgcgc agagcaatgg cattggcaac
     4441 cattatggca acagcttttg tggtatgtca actcataggt tggggtgaac tgggaaaagc
     4501 cggtattctg atggatactt ccacctctgc ttcttatttg tatttgctct ccggtttgca
     4561 tcttttgcat gttggcgtgg ggataataat cctgttggtt ggttgtgtgc gcttattcac
     4621 aaatacatct acgcctgtaa aagcccttct ctattatgca gatccagtgc gacgcgatag
     4681 attgaaatta ttaaatcatt attggcatac catcgatttc ctttggttat tcttgttttt
     4741 ggtgatactt tataatcaaa cataaacgcc ttgaaataaa aagccccctt accctgagag
     4801 gatagaaggg ggcttgtttc actccttaac caactatatt actcgttctt tttcttgtcg
     4861 tgcttgcgtt gcaatttggc ttttgcttcc tctttgctgt cagcatgcgc cttgatctcg
     4921 tctttatgcg cttttacagc ttcttttttg tcagcctctg ttttgtttgc ttttgcatct
     4981 tgagcatgcg cttttacttc agcttttgaa gcatcatgtg cctgaatctc atcctgatgt
     5041 gcctttacac gagcttttgc aggttttgca ttcattttgc cattcatggc agctttcttt
     5101 ttagcttgag caggtgtgat agcctgacgc ttttcaggag caactttctt cacctcagtt
     5161 ttcttagcct ccttattggc atctccgctc ggagcactct gagcaaacac ttgtccggca
     5221 gttacagcca gtacaaaaga taaggtcatc aatagctttt tcatatgcat tagtttttag
     5281 tttaattgct aatatgtgat acattcccaa aaccgtgcca acaccttctt aatgatgtta
     5341 aggcttgtta tgaattcccc ggaaagcctg aaaataaggt ttttacggca ctttgacatt
     5401 ctcaatcgcc atccctacct ttgcccacgg tcgatgcggg atgtagctca gcccggtaga
     5461 gcacacgtct ggggggcgtg gggtcgcaag ttcgaatctt gtcatcccga ccattggaaa
     5521 gctgcctgat ggtggctttt tacattttac atagcatcag ctcaatcgga tttttaacac
     5581 tttttatcat tgttgaattt tacctctgtt tatgtggtat tcttctacgc aaaaggtcat
     5641 gctttattaa tcgtgcaatt attcatcgcg ttatactaat tttacccccg gcagagttta
     5701 tcatcaatat cgccattcat gcaattcgtt taccccgcat ttctgtttgc actcgcaaca
     5761 ctggcaatac cggtggtgat acacctgttt aattttcgtc gatttaaaac aatttatttt
     5821 tccaatgttc gattcctaag aaatgtacag gaagaaacag caacgaaaaa taaactcaaa
     5881 cacctactgg ttctcttaac gcgttgtctc gctttagcag ctcttgtatt tgcatttgct
     5941 caacctttta ttcccgcaaa aaaatccggt aataattcat ctgatcgcgc agtaagtatc
     6001 tatatcgata actcatttag catggaagca gaagttaacg atgagcgcct gattgacatc
     6061 gctaaacgaa aagcagaaga aattgtgaac ggatttacta tcgatgatag atttcaactc
     6121 attaccaacg accttgaagc aaaacatcaa cgattggtca gcaaggatga aatgattcgt
     6181 tttatcgcag aagtgcaacc atctcctgtt gtaagaccac ttcaagaaat aagtgataga
     6241 caacgcgatg cattacaccg ggaaggagac aaagctgaaa aaatatcata cctgctaagc
     6301 gactttcaaa aaaatgcagg cgacattgat aacgatacta gcatcatcgt gaattttgtt
     6361 ccactgttgc agcgtgcaca aaaaaatgta gctatcgatt ccatgtggtt caccactccg
     6421 gtgcctgttt taaatcaacc gataagcatt tgcattcgcc tgcataatta tggtgatggc
     6481 gatgttgaaa atgttccggt taccctgcgt ttgaatgaac aaattaaagc tattgcagat
     6541 ataaatatca acgctaaagg cgatgttgtt gatacaatga ccttcacact taatagtggg
     6601 ggatggtaca gcggagagat cagtatcaaa gattatccgg tcacctttga tgatatgttg
     6661 tatttcagct tcaaacctgt tgaacaaatt ccggtgctgg ccattaatgg tggaagcgaa
     6721 aatattttcc tcaaaggttt ttttgggaat aataatttgt tccaattaaa aaataatccg
     6781 gcaagccaaa ttgcattcag cgaactgccc acttatgatt tgattatatt aaatgaagtg
     6841 ccttccatct ctggcggtct ggcagatgcc ttgcagcagc atttggctcg tggtggaaat
     6901 attatggtat ggccttcctt gacaatggat gtagcttctt atcaagcatt tttattggca
     6961 aataatgccg ctgtgtatgg ggcattaata aatagacctc gccctgtaag tgaagtggat
     7021 accaaaaatc cggtgtttgc agatgtgttt gaaaaaattc cacgcaacct ggcaatgccc
     7081 acggcaatga aaagctatgc actgcagaca agcaccggaa caagagaaga aatgttactt
     7141 cggttcaccg accgtcaggc aatgatggcg cgctatgcct caggtgatgg ctatatttat
     7201 ttatctgcag ttccgctcga tcgcacagtt actgatctgc cggtgcaggc aggattattt
     7261 gcaccgatta tgtataaagt tgctgtcagc aatgcccgcc gtctcccggc ttatgccaat
     7321 atcggtgaaa tgggatggat aaaccttccc ggaattcaat tacccggcga tgctaccatt
     7381 aaagttgaaa gcaaaggaaa cgaattcatc ccggagattc gcagaacagg caatagcgta
     7441 gcggtgaatc tcagcagcta tgtgcaacag gccggatggt atactatcgt accggataag
     7501 cagatggtaa acagcgatca attgttggcg ttaaattata atcgaagcga atctgacatg
     7561 tcatttgctg atgcatcggc gctgaaggag ttgtatgcag cctcaaatca ggagattatt
     7621 tcagatatcg accgcaattt tacgggaatg gtaacgcaaa tggccaccgg aacgcctctc
     7681 tggaaatatt gtattatatt tgtcctggtt ttcctcgcgg cagagattgc aattattcgc
     7741 ctgatgccgt agtaaacatc cgttatgcac tgcattcttc agggagccac catcatcgac
     7801 cccgcttcac agcatcatcg cagcactgtt tccattcgca tacagaatgg cgtaattacc
     7861 gaaattgcct catcaattgc tgataccgga gacgcgcagt tgatcgatct caaagggaat
     7921 tttgtttccc cgggttggat ggatatgctc gcagcttttg gcgatccggg acatgaatat
     7981 cgggaaacaa tacaaacagg tgccgctgcc gctgcaaaag ggggatttac tgccctcgga
     8041 cttatgccga atacacaacc tgccttacat ggtaaagcag agatcgaata cataaaaaat
     8101 catgcagccg gtcagctcac acatatattt ccctatggtg ccgtaacgca taatagagaa
     8161 gggaaagacc ttacagaaat atatgacatg catgctgcag gtgctatggc atttaccgat
     8221 gcagattcag caattcgtga tgcgggaatt ttattgcgca gcttgcaata tgtaaagccg
     8281 ttcaacggag taattatcaa tatcccaaac gaccataaaa ttgttggcaa cgcaaatgtg
     8341 aatgaaggtg caatgagtgt tcaattaggg atgtatggta ttcctgatat cctcgaagaa
     8401 cttatggtta ttcgcgatat taaacttgca gaatatactc aatcgcattt gcatatcggt
     8461 atagtttcat cagcaaaagt gttaccacat attcgtgcag cgaaagaaaa aggtctttcg
     8521 gtaacggcag gagttgctgc ttatcagttg tattttgatg aaacggaatt gcacgattat
     8581 aatgcgcatt ataaagtaaa tcctccgttg cgtacagctg ccgataaaca agccttgcgc
     8641 gaagctgttg cagatggcac tatcgatgtg gtgtgcagct accatttacc tcatgataat
     8701 gattcgaaag atgttgaatt cgaatatgca gcatttggaa tggcttcatt agaagttgta
     8761 ttcggcgcca tgaggtcagc acttccaaat ataagtccgg aacaaatcgt aaatattctg
     8821 gccgctaagc ctagaaatat tctcaatatt gcacaaccgg gaattgcagt tggaaatact
     8881 gcagacatca ccatgtttga tgcggaaact acctggacac ttcaacaaag tgacttgcga
     8941 tcaaaagctt caaataatcc ttttgttggt aaaacattga aaggaaaagt tttaggtgtc
     9001 attaacaaag ggaaaatctc aattgcatag gatggaaggg caggataaaa taatggacgt
     9061 gcaaccaatt gaacgcccaa gtcgcaaggg tcttatattg cgaaacgggt taatctttgt
     9121 tgcattacaa attgcaatta tgttggtaac ccattttcgc cgtgatagca ttagtgatca
     9181 aaacatgcgt attgcggtgg gaatgtacct gttattgtta gcagctattg ttgtggtgca
     9241 acttgatgca cgtatgaggg tttatagcgg tagaatggat tttggacagg cattttctac
     9301 aggaatgata ttcgttttgt ttgtaacagc attatttacg atatttattc ttgtgtttta
     9361 tctaactata ggaaaagagt tgcttgctca aatgattaca attgctgaaa actcattgcg
     9421 ggcaaatgga acagcagaag cagatattcc cgagcgaatg gaattttcaa ggaaaatatt
     9481 taccgctccg ggaatgagca tcattaccct tataggttat ttgttttttg gaacaataat
     9541 gagtttaatc ggtgcactgt tcactcaaag aaccaattaa tgcaaatcag tgtaatcata
     9601 ccactactca acgaagagga aagcctgcct gagctgtcgc aatggataag caaggtgatg
     9661 caggaaaata atttctcctg ggaagtgata tttgttgatg atggcagcag agataaaagc
     9721 tggcaggtag tgcaacaatt acatgccgat aatgcctcct tcaagggcat taaattccga
     9781 cgtaattatg gtaaatccgc agctttaaac gaaggctttg ccatggcgca aggcgatgtt
     9841 gttattacaa tggatgccga tttgcaggac agtcccgatg aaatccccgg attgtataaa
     9901 atgattgcag atgaagggtt tgatatggta agtggatgga aaaagaaacg tcatgaccct
     9961 attggaaaaa ctattccaag caaatttttc aattggacaa cggctcgcat caccggcatt
    10021 aaattgcacg attttaattg tggcttgaaa gcttataaac gcgatgtagt gaaaagtatt
    10081 gaagtgtatg gtgagatgca tcgatatata ccccagattg ctaaaactgc cggattctat
    10141 aaaatagggg agaaggtggt tgaacaccgt gcaagaaaat atggttattc aaagtttggt
    10201 tgggagcgtt tcatgaaagg atttctcgat cttacttcca tcatgtttgt gagtaagttc
    10261 gggaaaaaac ccatgcacct cttcggcact ttcgggttcc tgtcgttttt taccggtggg
    10321 atcattacca tatggcttat catagataag atcattaaaa tgaataccgg cgaaaaattc
    10381 cgacaggtta ctgatcagcc tttgttttat atcgcattag ttgccatgat agttggtgtg
    10441 caactttttg ttggcggatt tttaggtgaa ctcatttcaa gaaattctcc cgaccgcaat
    10501 caataccaga tcgatcaacg aatcggaggc gtatgaaaat attgctcata ggcacagctt
    10561 ggccatatcg tggcggactg gcctctttca atgagcgcct gattcaggaa tttattaccg
    10621 aaggtgatga tgcttcaata ataaccttca cattgcaata tccgggattt ctatttccgg
    10681 gaaaaacgca atatagtagt agtcctccac ccgggatacc tatcaccaga aaggtgaatg
    10741 ccgtgaatcc atttaattgg tggcgaattg gtaaggcaat tgcgaaagag aaacctgata
    10801 ttgttatcat aaaatactgg atgcctttta tggctccgtg ctttggtgcc attgcaaggc
    10861 atatcaaaaa gaataaacat agttccatta tttgtattgc ggataacatc atcccacatg
    10921 agcgcaaatt ttatgataag ccttttacca aatattttat ttctgcaatg gatgggtttg
    10981 tcctcatgag caaaagcgtg atggaagacc tcgcgcaatt cgacaggaaa aaaatacgtg
    11041 cgcttaaccc gcatccgctt tttgataatt tcggagatgg tgtttctaaa acggaagctt
    11101 tagctcgctt acatcttgag ttaaatcgca gatacatttt atttttcggg tttatccgcg
    11161 actataaagg actggatatt cttttggagg cgatgcatgc cattcctgaa gatgtacatc
    11221 tgattattgc cggtgaatat tacaccgata gtaaaccata cgatgcgtta attaatcaat
    11281 ataacctttc ggatcgtgtg catgcattta acaggtttat tccggacgag gaagtgaaat
    11341 attttttctg cgcagccgac ctggttgttc agccgtacaa acatgccaca caaagtggtg
    11401 ttacgcagat tgcctaccat tttgaagtgc ctatggtggt aacaaatacc gggggacttg
    11461 cagaaatggt tttggatgga caagtaggat atgtcactcc ggcagagcca cttgctatag
    11521 cacaagcagt gtcaaagttt tatgtggaag gaaagtcgga ggaattcaaa aggaaccttc
    11581 gagaagaaaa aagccgattt tcatggtcgc gtatgacatc tacgatacgc cggttgtacc
    11641 aaaaaggtta gtattatttg cttactgttg ctttttgttg ctgaagaaag taatcaggaa
    11701 aaataaaggc aagcccatca ggaagattaa aaatccatac tgagcagaag ttgcatcatc
    11761 cataaatgtg ctataggtga caaataataa caccaacata aaaactgcag gtatatacgg
    11821 ataccctttc atcttgaaac cactataatc ctttggtgag cggtgtctga aaataaacaa
    11881 tgcacctgct gcactggcaa gacttatgct atcaataaat aaaacatagg taacaatgtt
    11941 ttcaaaattg ccaagcaaga ataccgatgc aatcattact gccacaaaaa atgataaacc
    12001 aaattcttgc acctgagttt tgtgattaac cttgctgaat atttgcggca atactttatc
    12061 ttctgccatt gcatagtaaa cacgtgggtt actcatcatt gaggtgttta gaaatcccaa
    12121 tacagaagta aaaagtatta tggaggtgat cacagcgccg gtgttcccaa atagtgcctc
    12181 agccagatct ttggcaatca atggcgtatt tttcatttgt tcaaaaccaa gaacattcac
    12241 ataggcgagg tttatcagca aatacaatac aataacaatg cctacaccaa agaaaatggc
    12301 aaggggaata tttttttgtg gatccttcac atctgcacca aaattcactg tttgttgata
    12361 gccgccataa gtaaaaaata tagatatcaa tgcaatccca aaggcattta tgaaattcca
    12421 gtcctcatcg atgacaacag gaatatcagc atttgcctgt cctgaaaaga tcgcgaagca
    12481 acaagtaata atcatgacaa tttttagaag gctgagtaca ttctgtgcac gagcgcccat
    12541 tttaatacct gcataattga ttccaaataa aataagaata attacaatcg cagcggtgtc
    12601 gatcacaaca tagtcttcag ctgtgttacc gaacaatacc ggcgtcagat attcagctcc
    12661 aatgatggct acaccggcac tggaacctgc attgataatc acctggctcc agttcaacat
    12721 aaaggcatat atgggatgat aggcagtcga gaaaatacgg tagtaaccgc cggcactcgg
    12781 tagacgcgcc ccgatttcag caaaagtaag cgctccgaaa attgaaataa tacctcctgt
    12841 aatccatgca gcataaaaaa tccattcctt ccctgcactt tgtgcaacca tggcaggtgt
    12901 tctgaaaatt ccaacaccaa ttaccaggct cacaacaatc atggtgaggt cgaatctttt
    12961 gagttggggt tgaat
//
LOCUS       DAOQHY010000095        12914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2368_length_12914_cov_4.450268, whole genome shotgun sequence.
ACCESSION   DAOQHY010000095 DAOQHY010000000
VERSION     DAOQHY010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12914
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2368_length_12914_cov_4.450268"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="PLL28_08905"
     CDS             complement(<1..1005)
                     /locus_tag="PLL28_08905"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5106 domain-containing protein"
                     /protein_id="HNF69480.1"
                     /translation="MIRRFYILLTFTFLATALTAQSDTGHEIHVRIDGIRDTTIMLGH
                     HYGSKQYVIDTVRIDHNGDVVFAGNDTLPGGIYLVVVPQLKNRYFEMVISGQDQHFSM
                     HTDTLDFVKHMDVKGSTENSLYYQDVNFIAEKKDEMNAMKTKLDALPKDDPEAEKIKE
                     AMKELNKTVEDTRSAIIKNNPGLFYAKFLQAVTDVHIPEAPMNPDGTKDSTFALHYIQ
                     KHFFDNIDFNDERLLRTNLFDTRIHKYFEDYIYKVPDSIEKGVDFVIGKAAVNKKIFQ
                     YVTVMLLNDYATSKMMGFDAVYVYIVDKYYSTGKAYWLDDVGLYRIQQQAERVRPTLI
                     G"
     gene            complement(1017..2339)
                     /locus_tag="PLL28_08910"
     CDS             complement(1017..2339)
                     /locus_tag="PLL28_08910"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNF69481.1"
                     /translation="MTYPEALEYLFTKLPMYQRIGAAAYKKDLSNTLALLKALGDPHK
                     KFTSIHIAGTNGKGSVTNMLSSICMEAGYRVGWYTSPHLEDFRERIKVNGHMCHEQFV
                     VDFVQRMEPHIEAIQPSFFELTVAMAFEYFALQEVEIAVVEVGLGGRLDSTNVITPIL
                     SVITNISFDHMQMLGNTLQEIAGEKAGIIKNHVPVVIGEYLPETLPVFEQKAAEMGSP
                     IFPAEENVAIEVLSQMPGKLEVNVSYLGQLIYPELICGLSGEYQVRNLRTVIQSTEVL
                     MAKGIELPEEALYEGLRKVIDNTGFAGRWQWLQDHPTLIADCAHNSGGLAALFSQVNT
                     LTYENLHIVTGSVNDKDLDASLSLYPTSAIYYFAKPDIPRGLEAETLQNKAFGFGLKG
                     SVFDSVRDSLYAALNKAGKNDLVLICGSIFVVAEALPAYRDREKALSK"
     gene            complement(2431..3207)
                     /locus_tag="PLL28_08915"
     CDS             complement(2431..3207)
                     /locus_tag="PLL28_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNF69482.1"
                     /translation="MEKQGRTDTNNKVTSGFLTLGVTGGLLVAMLLLSFRPPNPPMVE
                     PEGLTVDFGQDLTGFGIEEPSSQISGGAPAHKPVVESAVTQQNESTITVPDKKNTTKP
                     TTQTTTTSTQQNTNTINSNELFDPSKLNNKGNSGSEGTAGGTGNEGSPDGDPNGYHGP
                     GAGRGDNGSVGWNLDGRSMKAPPTLQVDHNEIGDIRVKIYVDRNGKVTKAEYERSGST
                     ITDSYLISQSKNAAMKATFNVDKNAPEVQIGYMTFHFKLQ"
     gene            3550..4806
                     /locus_tag="PLL28_08920"
     CDS             3550..4806
                     /locus_tag="PLL28_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF69483.1"
                     /translation="MQTTTTSPFSGYQIFIIAIMAFLQFTIVLDFMVLSPLGAILMPE
                     LNITPSQFSHLVSAYAYSAFASGILSAGFADKFDRKKILLFFYVGFIGGTLMCGLAND
                     YHALLIARIITGLFGGVIGSVVFAITTDLFAMQVRGRVMGFIQMSFAASQVMGLPVGL
                     YLANKMNWHFPFYLIVAIGSIVFFIISIKMKPVNAHLAIKRDISPLKHLLKTLSRKRY
                     ITGFSATVLLATGGFMLMPFGSAFAVNNLGLTMEQIPLVYMVTGICTLIAGPFIGKIS
                     DRVGKWSVFLAGTILTMILVLFYCNLGITPLWLAITVNSILFIGITSRMIASSALMSG
                     VPDPQDRGSFMGLNSSISQLAGGMAASLAGLIVVQEDNGHLLHYPILSYVVVFAMMIT
                     LVMMYRIHKMVTPTITPSINPEISAS"
     gene            4830..5177
                     /locus_tag="PLL28_08925"
     CDS             4830..5177
                     /locus_tag="PLL28_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69484.1"
                     /translation="MQVKSLLSAALVSALVLTACNSTKQAGTTPAITYSGKVKTIIDE
                     NCANTCHNAARPAAGIDLTTYEKVKDQALNGKLIPAIQHAEGADPMPKKAAQLDDATI
                     KVIVDWAASGALQ"
     gene            5261..6817
                     /locus_tag="PLL28_08930"
     CDS             5261..6817
                     /locus_tag="PLL28_08930"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNF69485.1"
                     /translation="MHSKIAHARFTVRSILKHDLPASISVFFVALPLCLGVSLASNAP
                     LYSGLLAGIIGGILVPFISKSALSVSGPAAGLTAICAAAIAATGDIQTFFLSVMLAGV
                     LQTLLGVFRLGGFTHFIPSAVIKGMLSAIGVMLISKQVPLLIGYDEPDFWTNAFFNVF
                     TLDHAFDNILTVINHTSAGAIIIAAICVGIIIVWNKLSKKPSSVPQAFIIVIIGSSVA
                     AIFKAYMPTLALHDGQFVRIPSHVISQITIPDPQLLFSDSVIWRNAVIICFVATLETL
                     LSITAIDKLDNKNRITPQNRELVAQGFANFISGMVGGLPITAVIVRSSANVQAGAQSK
                     WSAVLHGVWLLIAILFGVYLLDFISYCVLAVILIQTGYSLAKPHIFKSVYRQGNEQFM
                     PFIVTFFAILFTDLLIGVGIGISYAIYFLIKHTYRAGYSLQVIHHGHTTVYKIELALN
                     VSFMNKKRILDMLESIPSYSEVEINGVDSVYIDRDVLEVIQEYRSKAKSRHIQLTTFG
                     IAEVETLVAH"
     gene            6888..8675
                     /locus_tag="PLL28_08935"
     CDS             6888..8675
                     /locus_tag="PLL28_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF69486.1"
                     /translation="MKILFQYLRPYRGLVILVLTLAAINTGFSLMDPILLGRLVNLAN
                     DFIGDKENYDWHRYIYSFSWNSPGVLLLLLSSVTVAMVSRIAKNFQDYFLNVVIQKFG
                     ASVFTDGLKHAMKLPYQEFEDQRSGETLSILTKVRTDVERFMNMAINVLLGILVGIVF
                     VFVYAAIFIHWYIPAVYLVGIVLLATISNVLSKKVKIIQKTIVGKTTALAGATTESLR
                     NIELVKSLGLTQQEVERLNRNTYKILGLELTKVKRIRSISFIQGTFVNTLRQVILFLL
                     MWLIFDDKMDAGQLVTMQVFSFFVFGPLQDIGNIILSYREAEASLLNFNNLMNKVPEP
                     EPASPKALGPIQHLQFDQVQFKHKTASTNAIDNISFEVNLGETIAFVGPSGSGKSTLM
                     KLLVGLYRPQNGKILYNGLNEFDINFNDLRNQIGFVTQDTQLFAGTIRENLMFVNPQA
                     TESDLQDVLQKASCNGLLSRAENGLDTMIGEGGLKLSGGEKQRISIARALLRRPRLLI
                     FDEATSALDSLTEEDISNTIREISAAGDQITILIAHRLSTIMHADRIYVLEQGEVEET
                     GTHDTLLENKGLYFAMWRQQIGGRKKVTV"
     gene            8992..11550
                     /gene="gyrA"
                     /locus_tag="PLL28_08940"
     CDS             8992..11550
                     /gene="gyrA"
                     /locus_tag="PLL28_08940"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNF69487.1"
                     /translation="MADGGRIIPINIEEEMKTAYIDYSMSVIVARAIPDVRDGLKPVH
                     RRVLYGMKDLGMAYNKPYKKSARIVGEVLGKYHPHGDSSVYDAMVRMAQDWSMRYTLV
                     DGQGNFGSVDGDSPAAMRYTEARFTRIAEEMMEDIEKDTVEFTNNFDDTLEEPTVLPS
                     RIPNLLINGSSGIAVGMATNILPHNLTEICDGIIALINNREITTTELMQYVKGPDFPT
                     GGVIYGVEGIIESFETGRGRIVVRGVANIETTANGREQIVITELPFQVNKALLISKIS
                     ELVHEKRIEGISDANDYSDREGIRVVIDLKKDAVATVVLNQLFKYTPLQSSFGVNNIC
                     LHAGRPKLMNLKTMIGHFVDFRHEIIVRRTRYDLKKAEERAHILEGLLIALDNLDAVI
                     ALIRAAKTPDEARDGLMTQFGLSEIQAKAILEMRLQRLTGLERDKIRDEYTEVMELIA
                     KLKEILDREDLRMQIIIDETLEIKEKYGDVRKTSIVAADGEMSIEDLIEEEDVAITIS
                     HLGYIKRTSLTEYRVQSRGGKGSKGGATRDEDFIEHIFIASTHDTMLFFTQQGKCFWL
                     PVYQIPEGTRVSKGRAIQNLISLPPEDKVMAYIDIHDLEDEAFINGHYLVFCTKKGTI
                     KKTTVEAFSRPRANGIVAINIEEGDELLEVRLTNGNCEIMLAVKSGRSIRFPEEKVRP
                     MGRTATGVIGIELANESDEVVGMITVEKGDKEKTVLVVSENGYGKRSYLVDPETGEDE
                     YRITNRGGKGVTTIKITPKTGNLIAIKDVTDRDDLMIICESGMTIRTGVEDLRVMGRA
                     TQGVRLINLRDGDSIASVTRVEKDDEEETSGAEGEEPNVDNPDTTSDTTSDNNE"
     gene            11585..12772
                     /locus_tag="PLL28_08945"
     CDS             11585..12772
                     /locus_tag="PLL28_08945"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNF69488.1"
                     /translation="MKRFATIMFAAMLGITGVQGQNSKITTAASAQYDREFGKAKQAI
                     DEACMHEKTKLEAKTWYMRGDIHNDIARDTTGRFNDIANPADTALASFKVALQQADVK
                     NYKLKIADGLFITYNIFFVKGANAYSAGNYEEAYTNFTKANEANLLQIDANPLASLDT
                     GVMFNMGLMAEKTNRTVEAIGVYQKLVDMKYGEPYLYSRLSSMYLDAGRQDDALKVLD
                     SGRKNFPTDKEIMTAELNYYLAQNKLGELVGKLENAIELDPKNAELYFVLGTTHGELV
                     KLDSLHGKQHFDAAVTAYNKALEIDKNRFDINLNAGALFYNTAIELNKRMNVLPLEME
                     AEFEKLKTERNSLYQNALPYFENAHAIEPKNTDCMIALKEIYVRLGQLDKAESIKQQL
                     DSN"
BASE COUNT         3765 a         2635 c         2799 g         3715 t
ORIGIN      
        1 acctatcaag gtggggcgta ctctttcagc ttgctgttgt atgcgataaa gtccaacatc
       61 atcgagccaa tatgctttac cggtagaata atatttatca acgatgtaaa catacactgc
      121 atcaaaaccc atcattttag atgtagcata atcgttgagt aacatcactg ttacatattg
      181 aaatattttt ttgttgactg ctgcttttcc tattacaaaa tcaacacctt tttcaattga
      241 atccggaacc ttataaatat agtcttcaaa gtatttatga atacgcgtat caaataaatt
      301 ggtacgtaac aatcgctcgt cgttgaaatc aatattatcg aaaaagtgct tttgaatata
      361 gtgcaatgca aacgtcgaat cctttgttcc atcggggttc ataggcgcct ccgggatatg
      421 cacatcagta acagcctgaa gaaatttggc ataaaaaagt ccgggattat ttttgatgat
      481 ggctgaacgt gtatcttcta cagtcttgtt tagttccttc attgcctcct taattttctc
      541 tgcttcagga tcatcttttg gcaaggcatc caatttagtt ttcatcgcat tcatttcatc
      601 cttcttttca gcaatgaaat tgacatcctg gtagtataac gagttttcgg tagatccctt
      661 aacatccata tgcttgacaa aatcaagcgt atcggtatgc atgctgaaat gttgatcctg
      721 tccgctgatc accatttcaa aatatctatt tttcaactgt ggcactacta caagatatat
      781 tccgcccggt agtgtatcat tacctgcaaa aaccacatcg ccattatgat caatgcggac
      841 ggtgtctatc acatactgtt tgctgccata gtgatgaccc agcataatgg tagtgtctct
      901 gatgccgtct attctgacat gaatttcgtg acctgtatca gattgtgctg ttaaggccgt
      961 tgctaaaaag gtgaaagtga ggagtatgta aaatcttcga atcataattg gtttcgctac
     1021 tttgataacg ccttttcccg atctctatat gccgggagtg cctctgctac cacaaaaata
     1081 ctgccgcata ttaatacaag gtcatttttg cctgctttat tcagcgctgc atataatgaa
     1141 tccctcacag aatcaaacac cgaacccttc agtccgaagc caaaagcctt attttgtaag
     1201 gtttcagcct ctaatcctct gggaatatcg ggtttcgcaa aataatatat ggccgaagtt
     1261 ggatacaagc tcaaagaagc atcgagatct ttgtcgttca cagatccggt tacaatgtgc
     1321 aaattttcat acgtaagcgt gttcacctga ctgaacaatg cagccaatcc accgctgtta
     1381 tgcgcacaat ctgcaataag ggtggggtgg tcctgcaacc attgccatct tcctgcaaaa
     1441 cctgtgttat caatcacttt cctcaatcct tcatatagtg cttcctcagg taattctata
     1501 cccttagcca tcaacacttc agtggattga ataactgtcc gcaaatttct gacctgatat
     1561 tctccggata agccgcatat cagttcaggg tatatgagtt gaccgaggta gctaacattt
     1621 acttctaatt taccgggcat ttgagatagc acttcaatgg ctacattttc ttctgcagga
     1681 aatattggtg atcccatttc agcagctttt tgctcgaata cgggcaaggt ttcaggcaag
     1741 tattcgccaa ttactaccgg aacatgattt ttgatgattc cggctttttc tccggctatt
     1801 tcctgcaagg tattgcctag catttgcatg tgatcgaaac tgatattggt gatcacagat
     1861 aatattggtg taattacatt ggtagaatcc aaacgcccgc ccagacctac ttccacaaca
     1921 gctatttcta cctcctgcaa ggcaaaatat tcaaatgcca tggctacggt caattcaaaa
     1981 aacgaaggtt gaatagcctc aatgtgcggt tccattcgtt gcacaaaatc aaccacaaat
     2041 tgctcatggc acatatggcc atttacctta atacgctccc ggaagtcttc gagatgtggg
     2101 gacgtatacc aaccaacacg ataaccggct tccatgcaaa tactcgatag catattagta
     2161 acagaaccct tgccgttagt gcctgcaata tgaatggacg tgaatttttt atgtggatct
     2221 ccaagtgctt tgaggagtgc taacgtgttt gaaagatctt tcttatacgc tgccgcaccg
     2281 atccgttgat acatcggcaa ttttgtgaat agatattcca gtgcttccgg atacgtcata
     2341 accgctgcaa atagacgtaa aaaggcttga aatcgaaagt atttgcacaa aaaaagcctc
     2401 ccgaaggagg cttcaattat ttcaaactgt ttattgcaat ttaaagtgga aggtcatgta
     2461 accgatctgc acttcagggg catttttatc gacgttaaac gtagctttca ttgcagcgtt
     2521 tttactttga ctgatcagat aactatctgt gatggttgag ccactgcgtt cgtattctgc
     2581 tttggttact tttccatttc tatccacata tatctttacg cgaatatcgc caatctcatt
     2641 atggtctact tgaagggtag ggggcgcttt catactgcga ccgtcgaggt tccaacccac
     2701 agaaccatta tcgccgcgac ctgcaccggg accatgataa ccattgggat caccatcagg
     2761 acttccctcg ttaccggtac cacctgcagt tccctcgcta ccggaattac ctttgttatt
     2821 cagcttgcta gggtcaaata actcgttaga gttgatggta ttggtattct gctgcgtaga
     2881 ggtagtagta gtctgcgtag taggcttagt tgtatttttt ttgtcaggga cagtgatagt
     2941 agactcattt tgctgcgtta cagccgattc taccaccggt ttatgagccg gagcaccccc
     3001 ggaaatttga gacgaaggct cttcaatgcc aaatccggtc aaatcctgcc caaaatctac
     3061 tgtaaggcct tcaggctcta ccataggcgg gtttggcggt ctgaaactca aaagtaacat
     3121 tgccacgagt aatcctcccg taacaccaag cgttaaaaat ccgctcgtta ctttgttgtt
     3181 agtgtctgtt ctaccttgct tttccatatg ctttcggttt tagatatgat gcaatgatga
     3241 tgtataatcc acaaaattca ttcctatctt gccgcaagtc aatgttttaa aacggttttc
     3301 gaccgaagga tagtatagga ttttacaaaa ttaagctact acacatctag tacaagcccg
     3361 acttcaacgt aataaatcga caatgtagct ttaaatagac gaggcataac aaaaattgca
     3421 gagtgcttgc acacattttc atgtgtatac atgatttttt catgttttca cttggattta
     3481 atattgcaga gaggtttaga tgaactaaaa gctattgatt agccttagca tcttttcagg
     3541 caactactta tgcaaacaac tactacttct ccgttttcgg ggtatcagat ctttattatt
     3601 gccataatgg cttttttaca gttcaccata gtcctcgatt ttatggtttt atcgccgttg
     3661 ggagcgatcc tgatgccgga actcaatatc accccttctc agtttagtca tctggtttcc
     3721 gcctatgcat acagcgcatt tgcctccgga atattaagtg cgggatttgc tgataagttt
     3781 gacagaaaga aaatactatt atttttctac gttggattta ttggtggcac cttgatgtgc
     3841 ggattagcca atgattatca cgccttattg atagcgcgca tcattacagg tttatttggc
     3901 ggtgtaatcg gatctgtggt cttcgcaatc accaccgatc tattcgccat gcaagtacgt
     3961 ggtcgtgtaa tgggattcat tcaaatgtcg tttgccgcca gtcaggtaat gggacttccc
     4021 gttggattat atcttgccaa taaaatgaac tggcattttc ccttttatct catcgttgct
     4081 attggcagta ttgtgttttt tattatttcc attaaaatga agccggtaaa tgcacatttg
     4141 gcaattaagc gcgatatttc tcctttaaaa catttactca aaacactttc cagaaagcga
     4201 tatatcacag gtttttcagc aacagttttg ctggcaacag gtggttttat gcttatgcct
     4261 tttggtagtg ccttcgcagt aaataatctt ggactgacca tggaacaaat accattggtt
     4321 tatatggtca ccggaatttg cacattgatc gcaggaccat ttattggtaa aataagcgat
     4381 agggtaggga aatggagtgt gtttttagcc ggaacaatac tgacaatgat cctggtatta
     4441 ttttattgca atctgggcat cacaccactg tggttggcta ttacggtaaa cagcatttta
     4501 tttattggta ttacttcccg catgatagcc tcttcggcat taatgtcggg agtacctgat
     4561 ccgcaagacc gcggttcatt tatgggattg aattcaagca tttctcaatt agcaggaggt
     4621 atggctgctt cattagcagg gttgatagta gtacaagaag acaatggtca cttattacat
     4681 tacccgatat taagttacgt tgttgttttt gccatgatga tcactttagt gatgatgtat
     4741 agaatacata aaatggtgac accaactatc acaccttcca tcaatccgga aatttctgca
     4801 tcttagtaca ttattttaat acgaacacca tgcaagttaa atcactatta tccgcagctt
     4861 tggtaagtgc attagtactt actgcttgta attccacaaa acaagcaggc acaactccgg
     4921 ctattaccta cagcggaaag gtaaaaacca tcatagatga aaattgtgcg aacacctgtc
     4981 ataacgcagc ccgacctgcg gcaggaatcg acctcacgac atatgaaaag gtgaaggatc
     5041 aagcacttaa tggcaaactg atccccgcca ttcaacatgc agaaggggca gatccgatgc
     5101 ccaaaaaagc cgcacaactc gacgatgcaa caatcaaagt gatagtagat tgggctgctt
     5161 ccggagctct tcaataagct tcttacaggc ggttatacac agaaaaggtt gcataatttc
     5221 ctacccaatt gggtaaggag tgctgcacct ttgtgataac atgcatagta agattgcgca
     5281 cgcacgcttt accgtaagat caatcctgaa acatgatctt ccggcaagca taagtgtatt
     5341 ttttgtggcg ctgccacttt gtcttggcgt ttcattagca agtaatgcac cgctttattc
     5401 cggattactt gcaggtatta taggtggtat tctggtgcct tttattagca aatctgcttt
     5461 gagtgtaagt ggacctgctg cgggattaac tgcaatatgt gctgccgcaa tagccgctac
     5521 cggcgatatc caaacattct ttctatcagt aatgcttgcc ggtgtgttac aaaccttatt
     5581 aggcgttttc cgattgggag gattcacaca ttttattcca agtgctgtta ttaaaggaat
     5641 gttatcggca ataggtgtaa tgcttatctc caaacaagtt ccattgctga ttggttatga
     5701 cgaaccggac ttttggacga atgcgttttt caatgttttc acacttgacc atgcgtttga
     5761 taatatatta actgttatca atcatacttc tgcaggggca ataattattg cagcaatatg
     5821 tgtaggaatt attattgtat ggaataaatt atcgaaaaag ccatcctctg ttccacaggc
     5881 atttatcatc gtaattattg gatcttctgt agcagcaata tttaaggcat atatgcctac
     5941 acttgccctg catgacgggc aatttgtgcg cattccttct catgtaatta gtcagataac
     6001 catccctgat cctcaactgc tttttagcga tagtgtaatc tggcggaatg ctgtcattat
     6061 ttgttttgtt gccactttgg aaacgctact cagtattact gcaattgata aactggacaa
     6121 taaaaatcgc atcactccgc agaatagaga gctcgttgca caaggatttg caaactttat
     6181 aagtggcatg gtcggtggtt tgcccatcac tgcagtaatt gtccgcagct ctgcgaatgt
     6241 gcaggcaggc gcacagtcga aatggtcggc ggtattgcat ggcgtgtggt tgttaattgc
     6301 catattattt ggcgtttatt tgttggattt catttcttat tgtgtacttg cagttatatt
     6361 aattcaaacc ggatattcgc ttgcaaaacc gcatattttt aaatcagtat accgacaggg
     6421 caatgagcaa ttcatgccat ttattgtgac attttttgct attttattca ctgatttgct
     6481 tattggtgtg ggtataggta tcagctatgc catttatttt ttaatcaagc atacatacag
     6541 agccggctat tcattgcaag taattcatca cggacatact actgtatata aaatagaact
     6601 tgcattgaat gtcagcttca tgaataagaa aagaatatta gatatgttgg aatcaattcc
     6661 ttcatacagc gaagtagaaa tcaatggggt ggacagtgta tacatcgacc gagatgtatt
     6721 ggaagtgatt caggaataca ggtcgaaggc aaaatccaga catatacagc tcaccacttt
     6781 tggtattgcc gaagtagaaa cactggtagc tcactgattc ttgataaaat aattactatt
     6841 caacgaacct tatagcttag ttttgagttg aaacccggca tactattatg aaaattttgt
     6901 ttcaatacct tagaccttac cgaggcttgg tcatccttgt gctcacactt gcagctataa
     6961 atacggggtt ttcactcatg gatcctattc ttttgggtcg attggtgaac ctggccaacg
     7021 actttatcgg ggataaagaa aattacgact ggcatcgcta tatctacagc ttttcctgga
     7081 atagtccggg tgttttgctg ctcctgctca gctcagttac cgttgccatg gtgagcagaa
     7141 ttgctaaaaa cttccaggat tattttctca acgttgtaat tcaaaaattt ggtgcaagtg
     7201 tttttaccga tggtttaaag catgcgatga aattaccata tcaggaattt gaagatcagc
     7261 gcagtggcga aacactttcc attttgacaa aagtgcgcac tgatgttgag cgattcatga
     7321 atatggctat taatgtgctg cttggcattt tggtggggat tgtatttgtg tttgtatatg
     7381 cagccatttt tattcattgg tatatcccgg ctgtatattt ggtaggcatc gttttgctgg
     7441 ctacaatttc taatgtgttg agtaaaaaag taaagattat acaaaagaca attgtgggta
     7501 aaacaacggc tttagccgga gcaaccactg aatccttaag aaatattgag cttgtgaaaa
     7561 gcttaggatt gacgcagcaa gaggtagagc gattaaatag aaatacatat aaaattctcg
     7621 gactggaatt aacaaaagtt aaacgcatca gaagtattag ttttatacag ggaacatttg
     7681 taaataccct gcgtcaggtt attttatttt tattgatgtg gttgatattt gatgataaaa
     7741 tggatgccgg acaattggta actatgcagg tattttcatt ctttgttttc gggccattgc
     7801 aagatattgg caatatcatt ttatcctatc gcgaagcaga agcatcatta ctgaatttca
     7861 acaacctgat gaataaagta cccgagccgg aacctgcttc accaaaggct ttgggtccaa
     7921 tacaacatct gcagttcgac caggtgcaat tcaaacacaa aacagcatct accaacgcaa
     7981 tcgacaatat tagttttgag gtaaaccttg gtgaaacaat tgcatttgtc ggcccttcag
     8041 gatcgggaaa aagcactttg atgaagttgc ttgtcggttt atatcgaccg caaaacggaa
     8101 aaatattata caatggtttg aatgaatttg atattaactt caacgacctg agaaatcaga
     8161 tcggatttgt tacgcaagac acacaattat ttgccggtac cattcgcgaa aacctcatgt
     8221 ttgtgaatcc acaggcaaca gaatcggatt tgcaggatgt attgcaaaaa gcaagttgca
     8281 atggattatt gtcacgtgca gaaaatggat tggataccat gataggagag gggggattga
     8341 aattatcggg cggagaaaaa caacgtatca gtattgccag agcattgttg cgcagaccga
     8401 gactattaat tttcgatgaa gcaacttcag cgttagactc acttactgaa gaagatattt
     8461 caaatactat cagagaaata tcggctgcag gtgatcaaat cactatatta attgcacatc
     8521 gcctgagtac cataatgcat gccgacagga tttatgtgct tgagcaagga gaagttgaag
     8581 aaaccggcac acatgatacc ttgttggaaa acaaaggatt atattttgcc atgtggcgcc
     8641 agcaaatcgg cgggcggaaa aaagtgactg tttaggaaac tacaaccttc aaattcctgc
     8701 aattttcccg gcattgtttt aaccatcttt aacaatgcca atgaaacttt tgggcagaaa
     8761 aggagtttat attatcaagt gtgttttcat ggggatcagg ggttgcgcca cgctcgtggg
     8821 cacccctgtt tttgtttttc tgcgcacaca aacttgcatt tatattttca agcccgtttc
     8881 cggttgaaaa atgtggaaaa cttgacagtc cgaaagccct ataatatggt gctttcgcgc
     8941 tgaaattgcc tacctttaca gcacctaaaa aaagcggaaa aagtagcata tatggctgac
     9001 ggaggaagga ttatcccgat taatatcgaa gaagaaatga aaaccgccta catcgattat
     9061 tcgatgtcgg taattgtagc gcgtgccatc cctgatgtac gcgatggatt aaagcctgtg
     9121 caccgtcgcg tgctttatgg catgaaagac cttggtatgg cttataacaa gccttacaag
     9181 aaatccgcac gtatcgtagg ggaggtgctt ggtaagtatc acccacacgg cgacagctct
     9241 gtttacgatg ccatggtgcg tatggctcag gattggagta tgcgatatac actggtcgac
     9301 gggcaaggaa acttcggttc ggttgacggt gatagtccgg cagcgatgcg atatacagag
     9361 gcgcgattta ctcgtattgc cgaagaaatg atggaagaca tcgaaaagga taccgttgag
     9421 tttaccaata acttcgacga tacccttgaa gagcctaccg ttttaccttc ccgtattcca
     9481 aacctgctga tcaacggttc ttcaggaatc gcggtgggaa tggccacgaa tatccttcct
     9541 cataacctca ctgaaatttg cgatggaatc attgcattga tcaataatcg cgaaatcacc
     9601 accactgagc tcatgcaata tgtgaaaggt cccgatttcc cgaccggagg tgttatctat
     9661 ggtgtagaag gcatcatcga aagctttgaa accggtcgtg gacgaattgt ggttcgcggt
     9721 gttgccaata ttgaaactac agctaatggt cgcgagcaga tcgtaatcac tgagctgcca
     9781 ttccaggtga acaaagcatt gcttatcagc aagatatctg aattggtgca cgaaaagagg
     9841 attgaaggca tttctgatgc caacgattac agcgatcgcg aaggaatccg tgtcgtaata
     9901 gacttgaaaa aggatgctgt cgccacggta gtactcaacc aattattcaa atacacgcca
     9961 cttcaaagct cttttggggt gaataatatc tgtctgcatg ccggcagacc gaaactgatg
    10021 aacctcaaaa ccatgatcgg ccatttcgtc gattttcgtc atgagataat cgttcgcaga
    10081 acgcgttacg atctgaaaaa ggccgaagaa cgcgcacata tccttgaagg cttgctgatt
    10141 gccctcgaca atcttgacgc agtgatagcg ctcatcaggg cggcaaaaac gccggatgaa
    10201 gctcgtgatg ggctgatgac acaattcggg ttatcggaaa ttcaggcaaa agccatcctt
    10261 gagatgcgtt tgcaacgtct tacagggctt gagcgcgata agatcagaga cgaatatacc
    10321 gaggtaatgg aactcattgc caaattgaag gagatactgg atagggaaga cctgcggatg
    10381 caaatcatca tcgatgagac actcgagatc aaagagaaat acggcgacgt tcgcaagaca
    10441 tcgattgtag ctgctgatgg tgaaatgagc atcgaagacc tgattgagga agaggatgtt
    10501 gctattacga tcagccacct aggttatatc aagcgtactt cactgacaga ataccgcgtg
    10561 caaagcaggg gaggaaaggg ttcaaaaggc ggagctaccc gcgatgagga cttcattgag
    10621 catattttca tagccagcac ccacgatacc atgttgttct ttacacagca aggcaaatgt
    10681 ttctggttac cggtgtatca gatacctgaa ggaacacgcg tttcgaaggg tagggcgata
    10741 cagaatctga tcagcctgcc tcctgaggac aaagtgatgg catatatcga tatccatgac
    10801 cttgaggatg aggcatttat aaatggtcac tacctggttt tctgcactaa aaaaggcacc
    10861 atcaagaaaa ctactgttga ggctttcagc cgtccgcgtg ccaatggtat cgttgccatc
    10921 aatattgagg aaggcgatga actgttagaa gtgcgcttaa ccaatggcaa ctgcgagatc
    10981 atgcttgcgg tgaaaagcgg ccgatcgatt cgcttccctg aagaaaaggt gcgtccgatg
    11041 ggccgtacag ccaccggggt gatcggaatt gaactcgcca atgagtcaga tgaagtagtt
    11101 ggcatgatta ctgttgagaa gggagataaa gaaaaaacag tgctcgtggt tagtgaaaac
    11161 ggatatggca agcgcagcta tctcgttgac cctgaaacag gtgaagatga atatcgcatc
    11221 accaacaggg gcggtaaagg cgttaccact atcaaaataa cccctaaaac aggtaatctc
    11281 atcgccatta aagatgtgac cgaccgcgat gatctgatga tcatttgcga atcaggaatg
    11341 accatcagaa ctggcgtaga agatttgcgt gtgatgggac gagctacaca gggagtgcga
    11401 ttaatcaacc tccgcgacgg tgacagtata gcttcggtaa cgcgtgtgga aaaagacgat
    11461 gaagaggaaa caagcggagc ggaaggcgag gaaccgaatg tggataatcc ggatacaaca
    11521 tcagatacta cttcagataa caatgaataa caaattgaat atcaatattt taaatcccaa
    11581 aaaaatgaag cgtttcgcga caatcatgtt tgctgcaatg cttggtatta ccggcgtgca
    11641 gggacaaaac agtaagatca caacagcagc aagtgcgcaa tatgatcggg aatttggtaa
    11701 ggcaaagcag gcgatagatg aggcttgtat gcatgaaaag accaaattgg aggcaaaaac
    11761 ctggtatatg cgtggcgata tccacaacga cattgcacga gatacaacag gccggttcaa
    11821 cgatatcgca aatcctgcag atacagccct ggcatcgttt aaagtagcct tgcagcaggc
    11881 ggatgtaaag aattataaat tgaagattgc agatggcttg ttcatcacct acaatatctt
    11941 ctttgtaaaa ggcgcaaatg cttactcagc aggcaactac gaagaagcgt atactaattt
    12001 caccaaagca aatgaagcca atctccttca gatcgacgcc aatccgcttg catcattgga
    12061 tacaggagtg atgtttaaca tgggactgat ggctgaaaag actaacagaa ctgttgaggc
    12121 tataggggtt taccagaaac ttgtggatat gaaatatggc gaaccatacc tgtacagccg
    12181 cttgtcgagc atgtatctgg atgccggtcg ccaggatgat gccctaaaag tattggacag
    12241 cggacgtaag aatttcccaa ccgacaagga gatcatgacc gccgagttga actattatct
    12301 ggcgcagaac aagttaggtg aattggttgg aaagcttgaa aatgctatag aattggaccc
    12361 taagaatgca gagctatact tcgtattggg aacaacccat ggcgagttgg tgaaattaga
    12421 ttcattgcac ggtaagcagc actttgatgc agctgtaacc gcttataaca aagcccttga
    12481 gatcgataag aatcgtttcg acatcaatct caacgccggt gcattgtttt acaataccgc
    12541 cattgaattg aacaaacgta tgaatgtgtt gccgttggaa atggaagctg aatttgaaaa
    12601 actaaaaaca gaacgcaaca gtctctatca aaacgcattg ccatactttg aaaatgctca
    12661 tgctatcgaa ccaaagaata ccgattgtat gattgcattg aaagagattt atgttcgcct
    12721 cggccaactg gacaaagctg aatcgatcaa gcaacaactt gatagcaatt gatagcacaa
    12781 aatcatttaa aagaagggga tccattgcgg atcccttttt ttattttagg aacctggtgg
    12841 taggtggctt aactataatc gacttaacat aatattaatt atgggcaaaa actggtaata
    12901 caagaataga gtcg
//
LOCUS       DAOQHY010000096        12722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2408_length_12722_cov_5.522855, whole genome shotgun sequence.
ACCESSION   DAOQHY010000096 DAOQHY010000000
VERSION     DAOQHY010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12722
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2408_length_12722_cov_5.522855"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1548
                     /locus_tag="PLL28_08950"
     CDS             <1..1548
                     /locus_tag="PLL28_08950"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNF69489.1"
                     /translation="IVTGNASMNMGDVTSSGFSANMFEDSLTNTLDNAAYSQSNNFSG
                     TAAASYIRKTNTKWKYFKVKANGSGGTSLNEENWNNLTTYFADGLTIADNSARTDDAY
                     DVTGALSLSGMYQLKKGFYLEPAVVAKMTAESLIRKQGPVDGEGITDSLSPSLLRNDY
                     IVRPTLAIKKNTEATQMRFELGVESDQLHLSVNDIEETPATYYYLLPGFMLEHEVKRG
                     LRFGIEYNSSVNTPTVSQLNPVTDYSNSLSLFTGNPFLKPEYVHDLQLRFMFFDQFSF
                     TSLFARLAGTYTVDKINYSKTINEDLSQSYTLVNVDNDMSGSLGLHFGTPIRPLKVNF
                     RLSADEGYNRGINYVNDVENVITSWTHGGEISFDNRKKDIIDMRIGGSMSYTTSQYSI
                     STGQNADYIKQSLFSELSITPDSLWRLEADGEMQAYRLIGDDETTIVPFVNAQLSRYI
                     LKNNRGTITLKAYDILNQNKGITQTAAYNYIQIQESNTMGRYFMLSFKYRINKEEQNS
                     GIDVSIN"
     gene            1741..2700
                     /locus_tag="PLL28_08955"
     CDS             1741..2700
                     /locus_tag="PLL28_08955"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNF69490.1"
                     /translation="MTILITGACGMVGSHMIDMYYAQGITDLVGTFYKPTTDMEDIHG
                     KARLVECDVRYYQRVHDIITGTKPDKIFHLAAQSYPTVSWHRPQETMDTNVTGTINVF
                     EAIKAVRKTDPDYDPVVVVACSSAEYGASLTPENTPVKEEAALLPLHPYGVSKVGQDL
                     LSFQYWQNDKIRCIRARIFNTTGPRKTNDVVADFTSRAVAIERKKANTLTVGNLNTRR
                     AITDVRDLVAALMLLSDKGNWGDVYNISGNKVYTISEIIPIIENYMGMSLTYEVDPKL
                     LRPTDEPIIYGDSTKLKNDTGWEQKYDLATTIRDMIDYLRSKP"
     gene            2703..3560
                     /locus_tag="PLL28_08960"
     CDS             2703..3560
                     /locus_tag="PLL28_08960"
                     /inference="COORDINATES: protein motif:HMM:NF015748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNF69491.1"
                     /translation="MMLNRTRLFDLDLINDHNFDAVISSMMDFSHEYDPLSDKLPLLF
                     TPNVDDVVKLNEKQYADLASVLRRSMYCLPDGQPIIWVSKLFKGRKLKRRLPGSELFP
                     LLWKEVISKQKRVMLVAPSEQVGELLKKEYPAMVYYVPPFFDVQDEVQLKKVQQEAAA
                     IYDSIMPELVFIGIRYPKQNHIALGLIDHHNRTYATPRGKEEKVGIRSYQLYGSNSAQ
                     PHALHLLMGASYEFYLGLKNRAPQFWQKIGMEWFYRFTQEPGRLFKRYFIDDLQFFPI
                     VMKEFFKSK"
     gene            complement(4219..4689)
                     /gene="tnpA"
                     /locus_tag="PLL28_08965"
     CDS             complement(4219..4689)
                     /gene="tnpA"
                     /locus_tag="PLL28_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HNF69492.1"
                     /translation="MSSTYSQIYIQIVFAVKHRECLIRYSWEEELYKYITGILTNKQQ
                     KLLAINGMSDHIHIFIGMRPSCCLSDLVREVKKASTEFIKENNFTKSKFSWQEGFGAF
                     SYSHSSIDNVIKYIMNQKEHHKVKSFKEEYYAYLKEFEIAYKDEYLFEWIDTES"
     gene            complement(4990..6195)
                     /locus_tag="PLL28_08970"
     CDS             complement(4990..6195)
                     /locus_tag="PLL28_08970"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HNF69493.1"
                     /translation="MKYILFILFAAFCQPIHAQWTLLPVEGPQCCSQIWFSDPTHGYY
                     SGGDSTLLDSWDGGMIMETFDGGISWDTLFDPEGDENYPFNVVFVGDVGYTSNRYSNF
                     YRTENGGKDWELIELGGTNFLYNGLQMPDDSTVLIAGYSGEIFKITDNGENITRVLFL
                     QPHYQSFIGMQCIGSTCYAYTNDFIFRSSNAGDTWDLIYTSVEDAIKLLFIYPNHQIM
                     MATKNADFGFRLFRTKDSCATWDTLGVMDADVFAINDMAFQDSIGYFVGYDYLLKKTT
                     DSGRTWVSIPLDTIYDIPGDLHELQLLSADIAFIAGYEGLFYSMGVPTSFPTTDGKLD
                     LLLYPNPADSYVNILLPESSDISVFNAIGQLVYQINSQQHVYIDTHSWANGFYIAKSD
                     FGAKGFIVQ"
     gene            6554..7582
                     /locus_tag="PLL28_08975"
     CDS             6554..7582
                     /locus_tag="PLL28_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HNF69494.1"
                     /translation="MIIRSKAPLRIGLAGGGTDVSPYSDIYGGCILNATINKYAYATI
                     LPRNNGKIVLHAMDRNERIELDSISKLPIDGTLDLLKGVYNRIVKDFNHNAPLSFELN
                     TYVDAPPGSGLGSSSTLVVAIVGAFTEWLKLPLGEYDIAHLAYDIERKDLTMAGGKQD
                     QYAATFGGWNFMEFYKDDKVIVNPLRIRPQYLQELEFNIVMYYTGTSRLSSTIIEAQS
                     SNVTKKSDKSIEAMHNLKEQAYMMKEAILKGELNRIGDILDYGWKNKKATASGVTSDV
                     IDSIYESAIKAGATGGKISGAGGGGFMTFYCPGNTRYKVIEVLNTFGGEVHNLEFSKY
                     GLTSWEAK"
     gene            7567..8157
                     /locus_tag="PLL28_08980"
     CDS             7567..8157
                     /locus_tag="PLL28_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HNF69495.1"
                     /translation="MGSQIEKIKSVIAESIRVKNDILNNPEMIAKCEEIAERIAEAFR
                     QDKKVLFCGNGGSAADAQHLAAEFSGRFYFDRPPLDAEALHVNSSYVTAVGNDYGYDQ
                     VFSRVLKAKGRQGDFLFGISTSGNSGNILKAFEVARERGMVIVAMTGESGGKMKDNCD
                     YLLNVPSNDTPRIQESHIMLGHIICELVEASLFERP"
     gene            8154..8849
                     /locus_tag="PLL28_08985"
     CDS             8154..8849
                     /locus_tag="PLL28_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNF69496.1"
                     /translation="MISEAIVLAGGFGTRLQKVVAEVPKPMAPVAGKPFLHYIFDYLI
                     KNDITHAVLAVGYLREVIIDTYGDNYRSLKLTYSIETDPLGTGGGIMQACKYLQGNEA
                     MVINGDTFFDVDLHALTSFHQQQKALLSVALKRMENFDRYGTVETNDTHQITGFREKR
                     YMSEGLINGGIYCLNTAIFPEHIPQKFSFEKEILEKEIHAGRIFGMESQGYFIDIGIP
                     EDYARAQDDFKSR"
     gene            8881..9429
                     /locus_tag="PLL28_08990"
     CDS             8881..9429
                     /locus_tag="PLL28_08990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNF69497.1"
                     /translation="MQKPVPQIDQSWTLFLDRDGVLNRKIDHDYVRNWSQFDWLPGVK
                     EALAYFATRFGKIVIVTNQRGVGRGWMTLEDLNDIHTRLKQAVTDAGGRIDGIYFCTD
                     TTDEGSTHRKPKPGMAMDAKGDFPAIDFSKSIIAGDSGSDIQFGKNLGMYTVLIAPES
                     KHDADLTVPGLAAFARLLQQND"
     gene            9590..10993
                     /locus_tag="PLL28_08995"
     CDS             9590..10993
                     /locus_tag="PLL28_08995"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HNF69498.1"
                     /translation="MVRRYSQFLELVYFLVDIVLLNVSFLIAIFINFDRISKVSDDRK
                     FAMLLVAVNLIWFMVTTVTNYYKSEKERRTGYEHLVFRFLKVLAFQVMLTFTYLILIK
                     GYTFSRLQLGYTYIFFTVLDLGWRITFETYLKRYRARGGNYRRIIVIGTTQASLNFVK
                     EIQEHNEFGYRFFGYFDDPEKSMVEVEGSLEDVQSYCRKHNIDEVYFALHPSDMSEYV
                     DDLLNFCNDNLVRFRMVPEFSSYISKQFKKVNIEYYGSTPILSIRPEPLDNLYNRLLK
                     RLFDIAFALLFFIIIGWWLFPIIALFVKLSSTGPVFFIQKRSGEGNEEFWCYKFRTMK
                     VNAEADTKQATKGDPRITRVGAFLRKTNLDELPQFINVLLGDMSVVGPRPHMLKHTEE
                     YSKIVNNFMVRHFVKPGITGAAQANGYRGDTTDPVMMEKRVQYDLWYLENWSFWLDVK
                     IVFLTVWSMLKGNENAF"
     gene            10983..12611
                     /locus_tag="PLL28_09000"
     CDS             10983..12611
                     /locus_tag="PLL28_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69499.1"
                     /translation="MRFRLLTLLIAISTAASAQQTFLPLNRNIYAWYEPAVNEQGVLF
                     HTAIKPWRMQELQQVVTYDSVFHLKKVSDDKFIGKVWNFFGYDTILGVNTEDFHLRVD
                     PLINFAGGMDIENGGKGLWTNTRGISVMGDIGPERKVSFQATLRENQAKYPDYLVPWI
                     KYYNVVPGQGKVRSFKNSAFDYANATGYVSYTPSKYFNFELGHDKNFIGDGYRSMLLS
                     DNALYYPFFKITTTVWKIKYMNLYTEFDDAYNTLDDSVMVVTGLTRKFGSFHYLSWDV
                     FDWVQVGLFEGIVWPANDSTRYRGFDVNYINPIIFFRPIEFGLGSPDNALIGANIKFK
                     PVNNAIIYGQFLMDDLDVAAARGGEGYYRNKFGIQGGFKIYNPFKINNLMIQAEMNQA
                     NPYTYAHKEPVQNYAHYNQPLAHPLGANFREFIGIINYRFFERFYMEAKVQSAVIGYD
                     QHAGDHFGSNIFISDYDIPGFPDSYGNYIGQGLKTTIRSADLRGGFLINPSINLNLEG
                     QIFIRRFANDQEKINTTMFMLALKTDLFNSYYDL"
BASE COUNT         3775 a         2811 c         2603 g         3533 t
ORIGIN      
        1 attgtaacag gaaatgcctc catgaatatg ggcgatgtta ctagctcggg tttcagcgca
       61 aatatgttcg aggatagcct taccaataca ctcgacaatg cagcatattc ccaaagcaac
      121 aatttttcag gtactgctgc ggcaagttat atccggaaaa caaataccaa atggaaatac
      181 tttaaagtaa aggcgaatgg cagcggaggt acatctttaa atgaagaaaa ttggaataac
      241 ctgactactt attttgcaga tggattaaca attgccgaca actccgcacg cacagatgat
      301 gcttatgatg tgacaggtgc attgtctctt tcaggaatgt atcaattgaa aaaaggattt
      361 tacctcgagc ccgctgtagt agcgaaaatg actgcagaat cactcatccg caagcagggt
      421 ccggtagatg gagaagggat taccgacagt cttagccctt cattattgcg caacgattat
      481 attgttcgac ctacattggc gataaagaaa aataccgaag caacacagat gagatttgaa
      541 ttgggcgtgg aatcagatca attacattta tctgtgaatg atatagaaga aacaccggca
      601 acgtattatt atctgctacc gggttttatg ctcgagcatg aagtgaaacg cggactgcga
      661 tttggaattg aatataacag cagtgtaaat actcccactg tgagtcagct taatccggtt
      721 accgattata gcaatagcct ttcactattt acaggaaacc catttctaaa accggagtat
      781 gtgcatgact tgcaactccg tttcatgttc ttcgatcaat tctcattcac atcactcttc
      841 gctcgattgg ctggaacata taccgttgat aagatcaatt atagcaagac catcaatgaa
      901 gacctttcgc aatcatatac attggtgaat gtcgataatg atatgagtgg ttcactcgga
      961 ttacatttcg gtacgccgat tcgtccgtta aaagtaaact tcagactctc agcagatgaa
     1021 ggatataaca gaggtatcaa ttatgtaaat gatgtagaga atgtcattac ctcatggaca
     1081 catggcggcg agatcagttt cgataaccgt aaaaaagata tcatcgacat gcgtattgga
     1141 ggctcaatga gttatactac ttcgcaatat agtatatcca ccggtcaaaa tgccgattat
     1201 atcaagcaat cacttttctc agaattaagc attacccccg acagcctctg gcgacttgaa
     1261 gccgatggtg aaatgcaagc gtatcgtttg attggtgatg atgaaactac tatagtgcca
     1321 tttgtaaatg ctcaattatc acgctacatc ctcaaaaaca acagaggaac catcacccta
     1381 aaagcatatg acatcctgaa tcaaaacaaa ggcatcaccc aaacagcagc ctacaactat
     1441 atccaaatac aagaatcaaa tactatgggt cgctacttca tgctcagctt caaataccgt
     1501 atcaacaaag aagagcaaaa ctccggcatc gacgtaagca tcaactgatt aaagaagcca
     1561 tacacataca cctcatcatc ccatcacccc attacctcaa ccaacaattg gctaatcagc
     1621 taatcaacta atcggctaat cagctaatca gcaaatctgc caatcggcca atcggctaat
     1681 tggctaatcg gctaattggc taattcccaa taccccttat ctttgcacaa aatttcacca
     1741 atgacaattt tgatcaccgg cgcctgtggc atggtgggat cgcatatgat cgacatgtat
     1801 tatgcccagg gaatcaccga tctagtgggc acattttata agcctaccac cgatatggaa
     1861 gacatccacg gcaaggctcg attggtggaa tgtgatgtgc gctactacca acgcgtgcac
     1921 gatattataa ctggaaccaa acccgataaa attttccacc tcgcagcaca gagctatcct
     1981 accgttagct ggcatcgccc gcaggagact atggatacca atgtcaccgg caccatcaat
     2041 gttttcgaag ccataaaggc cgtgcgaaaa acagatccgg attatgatcc tgtagttgta
     2101 gtagcatgtt cgagtgcaga atacggtgca tcactgacac cggaaaatac ccctgtgaaa
     2161 gaagaagctg cattattgcc tttacatcct tatggtgtga gcaaagtagg acaagacctg
     2221 ctttcatttc aatattggca aaatgacaag atacgttgca tacgcgcgcg aatttttaat
     2281 acaacaggtc cacgcaaaac aaatgatgta gtggccgatt tcacaagtcg cgctgttgcc
     2341 attgagcgta aaaaagcaaa tacacttaca gtaggcaatc tcaatacgcg tcgtgccatc
     2401 accgatgtgc gcgacctcgt agcggcattg atgctattaa gcgataaagg aaattggggc
     2461 gatgtatata atatttcagg aaataaagtc tatacgatca gcgaaataat tcctatcatc
     2521 gaaaattata tgggtatgtc gctgacttac gaagtagatc cgaaattatt aagaccaacc
     2581 gatgagccta tcatttatgg cgatagcaca aaattgaaaa acgataccgg atgggaacag
     2641 aagtatgatc tcgctaccac catcagagac atgatcgact atctgcgcag caagccataa
     2701 tcatgatgtt gaatcgcacc cgtttatttg acctcgatct gatcaatgat cacaacttcg
     2761 atgcagtgat ttcatctatg atggattttt cgcatgaata cgatccgttg tcagataaat
     2821 tgccgctgct gttcacaccg aatgtcgacg atgtagtaaa actcaacgaa aaacaatatg
     2881 ccgatctcgc atcagtgttg cgcagaagta tgtattgctt gccagacgga cagccaatta
     2941 tttgggtaag taaactattc aaagggcgaa aactgaaaag gcgtttgccg ggaagtgaat
     3001 tgtttccttt gttatggaag gaagtcatca gcaaacaaaa acgcgtgatg ctcgtagcgc
     3061 catcggaaca agtaggggag ttgttgaaaa aagaatatcc cgcaatggta tattatgtgc
     3121 cgccattctt cgatgtgcaa gatgaagtac aattgaagaa agtgcaacag gaagcagcag
     3181 caatttacga tagcattatg cccgaattag tgttcatcgg cattcgctac cccaagcaaa
     3241 accatatcgc actcggactc atcgatcatc acaatcgaac ctatgccaca ccacgtggaa
     3301 aggaagaaaa agtcggcatc aggtcctatc aactttatgg cagcaacagc gcacaaccgc
     3361 atgccttgca tttattaatg ggcgccagct acgaattcta cctcggactc aaaaaccgtg
     3421 caccacaatt ctggcagaaa atcggcatgg aatggttcta ccgcttcacc caggaacccg
     3481 gcagattatt caaacgctac ttcatcgacg atctccaatt tttccccata gtgatgaaag
     3541 aatttttcaa gtcgaaataa ttttttaccg cgattataat atttaatttt tgggcgtatc
     3601 cctgcgggcc gggctatccg ctgcaagtcc tcattccgct tcgctgcatt ccgggctttc
     3661 cgctgctatc ccttacgcgg ataaatttgt tcatgtttga ttttgatttt tgcagtacat
     3721 gaatcgtatc atcaaaattt ttttattaaa catgtgaccc cttcggggtc ggggagatgg
     3781 caatgtgatt gattaattga aatgatgacc cctacggggt cgtttttgct gcggggatac
     3841 gcacacccga ccccggaggg gtcgcatgtt tgtatccccc gcgccgccac accacacccg
     3901 accccggagg ggtcgcatgt ttgtatcccc cgcgccgcgc cgccacaccg cacacccgac
     3961 cccgaagggg tcgcatgttt gtatcccccg ccccgtgccg ccacaccaca cacctgaccc
     4021 cgaaggggtc gcatgtttgt atcccccgtg ccgccacacc gcacacccga ccccggaggg
     4081 gtcgcatgtt tgtatccccc gcgccgcgcc gccacaccgt actcgacccc gaaggggtcg
     4141 catgtttatt tcgggattta ttttgttgac agcacaacac catcagaaat aaaatctacc
     4201 tgtttggtga tatccctttc agctttctgt atctatccac tcaaataaat actcatcttt
     4261 atatgcaatc tcgaactctt ttaagtaggc ataatactcc tctttaaacg attttacttt
     4321 gtgatgctcc ttttggttca tgatgtactt tataacgttg tcaatactcg agtggctata
     4381 tgaaaaagca ccaaaccctt cctgccagct gaacttcgat ttcgtgaaat tgttttcttt
     4441 gataaactca gttgaagcct ttttcacctc tcttaccaaa tccgataaac aacaagacgg
     4501 ccgcatgcca ataaatatgt gaatatgatc cgacatccca tttattgcta ataacttctg
     4561 ctgcttgttg gtgaggatgc cggttatata tttatacaac tcctcctccc acgaatatcg
     4621 tatcagacat tctcgatgct tcacagcaaa aacaatctgt atataaattt gcgaataggt
     4681 actcgacatg tagaactcaa tttttaaggg aatgacactt ttttctgcct gcatccacta
     4741 aatcaatatc atcatgttga taattcagct ctgccggata aaaatatcat ttaataggct
     4801 caaacctata caaatttcat gcactttccc ttaaattaga aattgaattt tttcgatgaa
     4861 taagaagtaa ataactcata tcacggagga aattttctgc atccggtaaa atttcagaat
     4921 gtaagcggtt agcagttatt taatcgataa taaaatattt acaattcgat tatacaatat
     4981 aagtcattat cactgcacaa taaatccctt cgcaccaaaa tcactcttgg caatgtaaaa
     5041 tccgttcgcc caggaatgtg tatctatgta tacgtgctgt tgcgagttga tctgataaac
     5101 gagctgcccg attgcattga agactgagat atcacttgat tcgggaagta atatatttac
     5161 atagctgtct gcaggattgg ggtaaagaag caagtctaat ttcccgtcag tagttggaaa
     5221 tgatgtggga acgcccatcg aataaaataa tccttcatat ccggcaataa atgcgatatc
     5281 agcacttaaa agctgcaatt catgcaagtc gcccggaatg tcgtaaatag tgtcaagggg
     5341 tatgctaacc catgtcctcc ctgagtctgt ggttttcttt agcaaatagt catatccaac
     5401 aaaataaccg atagaatcct ggaaagccat gtcattgatg gcaaaaacat cagcatccat
     5461 aacaccaagt gtgtcccagg ttgcacaact atctttagtg cgaaatagtc gaaatccgaa
     5521 atcagcattt ttagttgcca tcattatttg gtgattggga tatatgaata gcagtttgat
     5581 cgcatcttca accgatgtat aaattaaatc ccatgtgtca ccggcgtttg aactgcgaaa
     5641 aatgaagtca tttgtatagg catagcaagt agaccctatg cactgcatgc cgataaatga
     5701 ttgataatgc ggttgaagaa ataatacgcg ggtgatattt tctccattat ctgtgatctt
     5761 aaatatttca cctgaatacc ctgcaatcaa tacagtacta tcatcaggca tttgtaatcc
     5821 attatataaa aaattagtac cccccagttc aatcaattcc caatcttttc ctccgttttc
     5881 tgtccgataa aaattgctat atcgatttga tgtataacca acatcaccaa caaaaaccac
     5941 gttgaaagga tagttttcat cgccttccgg atcaaacaaa gtatcccagc ttattccgcc
     6001 atcaaatgtt tccatgatca tccctccatc ccaactatct aatagggtgg aatcaccgcc
     6061 actataataa ccatgcgtag gatcggaaaa ccaaatttgt gaacaacatt gcggaccttc
     6121 tacgggtaac aaggtccatt gggcatgaat gggctggcaa aaagccgcaa acaggataaa
     6181 tagtatgtat ttcatgggtg ggattactgc tttcaaagaa ataatttttt gatatttatt
     6241 gattttctga tagaaaatcg gtgctgttgt ttaatctaat gtatttcacc ctcataattt
     6301 tcattacccc tatttgccaa taattcccga cctttgcacc aaatttggaa gtaaggttta
     6361 ggcatcatgc aaccaaatat cagtattatt cggtctaccg gctacctccc gcccccaact
     6421 cgggaccccg caaaaccaaa tacctgatac ctgatacttg atacctgata cttgatacct
     6481 gatacctgat acttgatacc tgatacctga tacttgatac ctgataccag ataaaccaag
     6541 agataacaca aaaatgatca tcagaagcaa agcccctctc cgcatcggac tcgccggtgg
     6601 tggaacagat gtaagccctt acagcgacat ttacggcgga tgtatcctca atgccactat
     6661 aaataagtat gcctacgcta ccatcctgcc acgcaacaat ggcaagatcg tattgcatgc
     6721 catggaccgc aatgaacgca tcgaactgga tagcatatcc aagctaccta ttgatggcac
     6781 cctcgacttg ctgaaaggcg tttataaccg catcgtaaaa gattttaacc acaacgctcc
     6841 actttcattt gaactcaata cttacgtgga tgcgcctccg ggatccggcc tcggttcttc
     6901 atcgacactc gttgttgcaa tagtaggcgc tttcaccgaa tggttgaaat tgccacttgg
     6961 agagtacgac atcgctcacc tcgcttacga catcgagcga aaagacctta ccatggccgg
     7021 tggcaagcaa gatcaatatg ccgctacttt cggaggatgg aatttcatgg agttttataa
     7081 ggatgacaaa gtaatcgtta acccgctccg tattcgtcct caatacctcc aggaacttga
     7141 attcaatatc gtgatgtact ataccggcac aagccgtttg tcgtctacta tcattgaagc
     7201 gcaaagcagc aacgtgacta aaaaatctga taagtcgatc gaggctatgc acaatctgaa
     7261 ggagcaggca tatatgatga aggaggcgat cttaaaaggt gaactcaatc gcatcggcga
     7321 cattcttgat tacggctgga aaaataaaaa agctacggca tcaggtgtta ccagcgatgt
     7381 gatcgacagt atttatgaaa gtgctatcaa agcaggcgca acaggaggaa aaatctccgg
     7441 cgcaggtggt ggtggtttca tgacattcta ctgccccggc aatactcgat ataaagttat
     7501 tgaagtactg aacacctttg gtggcgaagt acataatctc gaattttcaa aatacggact
     7561 gacatcatgg gaagccaaat agaaaaaatt aaaagcgtga tcgctgagag catccgcgtt
     7621 aaaaatgaca tcttgaataa tcctgaaatg atcgctaaat gcgaagagat cgccgaacgc
     7681 attgccgagg cttttcgtca ggataaaaaa gtattatttt gtggcaatgg tggctctgca
     7741 gccgacgctc aacacttggc tgccgaattt tcaggtcgct tttatttcga tcgcccacca
     7801 ctggacgccg aagcattgca cgtgaattct tcttatgtta ctgctgttgg aaacgactat
     7861 ggttatgatc aggttttcag ccgggtatta aaagcaaaag gccgccaagg tgattttctt
     7921 ttcggcattt ccacttccgg aaattcaggt aatatcctga aagcttttga agtagcacgc
     7981 gaacgcggaa tggtgatcgt tgccatgaca ggtgaaagcg gtggtaaaat gaaagataat
     8041 tgcgattatc tgctcaacgt gccatcaaat gatacaccac gcattcagga aagtcacatt
     8101 atgctcgggc atattatttg cgaactcgtt gaagcgtcat tgttcgaacg tccatgatca
     8161 gtgaagcaat agtgcttgca ggtggctttg gcactcgcct acaaaaagta gttgctgaag
     8221 tgccgaaacc tatggcgcct gtagccggaa aaccttttct gcactatatt ttcgactatc
     8281 tcattaaaaa cgacatcacc cacgctgtat tggcggtcgg atatttgagg gaagttatta
     8341 tcgataccta tggcgataat tatcgctcat taaaacttac ctattccatc gaaacagatc
     8401 cgcttggaac aggtggtggc atcatgcagg catgcaaata tttgcaaggc aacgaagcaa
     8461 tggtaattaa tggcgatact ttttttgatg tcgaccttca cgcactcaca tcgtttcatc
     8521 agcagcaaaa agctttgttg agtgttgcgc taaagcggat ggaaaatttc gacagatacg
     8581 gaaccgttga aacaaacgat acgcatcaaa ttacgggatt tcgcgaaaag agatatatgt
     8641 ctgagggact tattaatggt gggatttatt gcctgaatac tgcaattttt cctgagcata
     8701 ttcctcagaa attctccttt gaaaaagaaa ttcttgaaaa ggaaatacat gccggtagaa
     8761 ttttcggaat ggagtcgcaa ggttatttta ttgatatagg aatacccgaa gattatgccc
     8821 gtgcacagga tgattttaaa tcgcgctgac cacttttaat cgccaaattc attttttaag
     8881 gtgcaaaaac cggttccaca aatcgatcaa tcatggacgc tattcctcga cagagatggc
     8941 gtattaaatc gaaaaatcga tcacgactat gtgcgcaatt ggtcgcagtt cgattggctc
     9001 cccggtgtaa aggaagcact cgcttatttc gccactcgtt tcggtaaaat tgtaattgtc
     9061 accaatcaac gtggggttgg tcgcgggtgg atgacacttg aagacctgaa tgacatccat
     9121 acacgcctca aacaagcggt tacagatgca ggcggtcgaa ttgacggcat ctatttctgc
     9181 accgacacaa ccgacgaagg gtcaacacac cgtaagccaa aacccggcat ggccatggat
     9241 gctaagggcg attttcctgc gatagacttc tcaaaatcca tcattgccgg tgattccggt
     9301 agcgacatac aattcggcaa aaacctgggc atgtacacgg tattgattgc ccccgaatct
     9361 aaacatgatg cagatttaac agtacccgga cttgcagcat ttgcaaggct tttacagcaa
     9421 aatgattaac ggttttccgg tattttcact gtttacgcat aaaatcttac ctccttttac
     9481 tatttttgca atagcgcttg gccctcaaca atcgctgcat gataacacac acgatgcaac
     9541 ctttgagatg tgaaaagcat cagaaaaggg tatcggagaa tgagcacaaa tggtaagaag
     9601 gtattcacaa tttctcgagt tagtttactt cctggtagat attgttctgc tcaatgtctc
     9661 ctttcttatc gccattttca tcaatttcga ccgtatttct aaggttagcg acgaccgcaa
     9721 gtttgctatg ctccttgttg ctgttaacct catttggttc atggttacta ccgttaccaa
     9781 ttactacaaa tctgagaagg aacgccgaac aggctatgaa catcttgttt tcaggtttct
     9841 caaggtgctg gcatttcagg tcatgttaac gtttacctat ctgatcctca tcaagggata
     9901 tactttttca cgtttacagc ttggatatac ctacatattt tttactgttc tggatcttgg
     9961 atggcgtatt accttcgaaa cttacctgaa gagatatcgg gcacgcggag ggaactatcg
    10021 ccgtatcatc gttatcggaa ctacccaggc ctcgcttaat ttcgtaaagg aaattcagga
    10081 acacaatgaa tttggatatc gctttttcgg ctattttgac gatcctgaaa aatcgatggt
    10141 ggaagtggaa ggtagtttgg aagatgtgca aagctattgt cgcaagcata atattgacga
    10201 agtgtacttc gcgctccatc cttctgacat gagtgaatat gtagatgact tgttgaattt
    10261 ttgtaacgat aatctcgtgc gcttccgcat ggtgcctgag ttctcctcat acatcagtaa
    10321 acaattcaaa aaagtaaata tcgaatatta cggcagtact ccaattttga gtattcgtcc
    10381 tgaaccattg gataatttat acaaccgttt gctgaaacgt ttattcgata ttgcctttgc
    10441 attattgttt ttcattatca tcggatggtg gttattccct atcattgcac tatttgtaaa
    10501 attatcttct accggaccgg tattttttat tcagaaaaga agtggtgaag ggaatgaaga
    10561 attttggtgt tataagtttc gcaccatgaa ggtgaatgcc gaagccgata ccaaacaagc
    10621 tactaaagga gatccgcgta tcacaagagt gggcgctttt ctgcgtaaaa ccaacctcga
    10681 tgagttgccg caattcataa atgtattact tggcgatatg agtgtggtgg gccctcgtcc
    10741 acatatgttg aaacatacgg aagaatattc caaaatcgtg aataacttta tggtgcgcca
    10801 tttcgtgaag ccgggtatta ccggtgccgc acaagccaat ggttaccggg gcgatactac
    10861 cgatccggta atgatggaaa aacgcgtgca atacgacctt tggtacctcg agaactggag
    10921 tttctggctc gatgtcaaaa tcgtattcct cacagtatgg agcatgctta aaggaaacga
    10981 aaatgcgttt tagattactc accctcctca tcgcgatcag cacagctgct tccgcacaac
    11041 aaactttttt accgctcaac cgaaacattt acgcatggta tgaacctgct gtaaatgaac
    11101 aaggtgttct gtttcatact gccattaaac cctggcgtat gcaagaattg caacaggttg
    11161 ttacctatga ctcggtattt catttgaaaa aagtaagcga tgataaattc atcggaaaag
    11221 tgtggaactt ctttggctat gatactattc ttggagtgaa taccgaagac ttccacttgc
    11281 gtgtcgaccc cctgatcaat ttcgccggag gtatggatat tgagaatgga ggaaagggtt
    11341 tatggacgaa tacgcgtggt atttccgtga tgggagatat cggtccggaa cgcaaagttt
    11401 cttttcaggc aacacttcgc gaaaatcaag caaaatatcc cgactatctc gttccatgga
    11461 ttaagtatta caacgttgtt cccggacaag gaaaagtgcg ttctttcaaa aacagtgctt
    11521 tcgattatgc aaatgctacg ggatatgtat cctatacgcc gagtaaatat ttcaatttcg
    11581 aattagggca tgataagaat tttattggcg atggttatcg cagtatgcta ttaagcgata
    11641 atgcccttta ttaccctttc ttcaagatta caactactgt gtggaagatc aaatacatga
    11701 acctttacac tgagtttgat gatgcctata atacgctgga cgattcagta atggttgtaa
    11761 ccggtcttac gcgcaaattc ggatcttttc attatttgag ctgggatgtt ttcgattggg
    11821 tacaagtggg tttatttgaa ggtattgttt ggcctgcgaa tgattcgact cgctatcgcg
    11881 gattcgatgt caattatatc aatccgatta ttttcttccg acctattgaa tttggtttgg
    11941 gctcacctga taatgcatta ataggcgcaa atattaaatt caaaccggta aataacgcca
    12001 tcatttatgg tcaattctta atggatgact tagatgtggc tgcagcaagg ggaggagagg
    12061 gctactatcg aaataaattt ggcatacaag gtggatttaa aatctataat ccttttaaaa
    12121 ttaacaattt aatgattcaa gctgagatga atcaggcaaa tccatacacc tatgcccaca
    12181 aggaaccggt gcagaattat gcccactata accagccact tgcccaccca ctcggagcca
    12241 atttccggga atttattggc attattaatt atcgtttttt cgaacgtttc tatatggagg
    12301 cgaaggtcca gtcggctgta attggttacg atcagcatgc cggtgatcat ttcggatcca
    12361 acattttcat ttcagactac gacatcccgg gcttccctga ttcctatgga aattacatcg
    12421 gccagggact caaaactact atccgtagtg ccgaccttcg aggtggattt ctgatcaatc
    12481 caagtatcaa tctaaacctc gaaggccaga tatttattcg tcgcttcgcc aatgatcagg
    12541 agaagattaa taccaccatg ttcatgctgg cgcttaaaac tgatttgttc aattcctact
    12601 acgacctcta ataacgcccg tttttcagta cctttgcacc cccaaaagtt ctttgattgg
    12661 tcccgtagtt caatggatag aatagaagtt tcctaaactt ttgatacagg ttcgattcct
    12721 gt
//
LOCUS       DAOQHY010000097        12512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2461_length_12512_cov_5.329614, whole genome shotgun sequence.
ACCESSION   DAOQHY010000097 DAOQHY010000000
VERSION     DAOQHY010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12512
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2461_length_12512_cov_5.329614"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /gene="rfbB"
                     /locus_tag="PLL28_09005"
     CDS             <1..884
                     /gene="rfbB"
                     /locus_tag="PLL28_09005"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNF69500.1"
                     /translation="KGDITDAGFITSLFEEHRFDMVVHLAAESHVDRSIANPIEFVIT
                     NVVGTCVLLNAARNIWKDDYSGKRFYHISTDEVYGTLGESGLFTETTPYDPHSPYSAS
                     KASSDHFVRAYHDTYGMPVVISNCSNNYGSFQFPEKLIPLAINNIKNMKPIPVYGKGE
                     NVRDWLWVEDHARAIDVILHNGKNGDTYNIGGFNEWKNIDLIHLLCRIMDNKLQREPG
                     TSAKLITYVTDRPGHDMRYAIDAQKLNRELGWKPSVDFEQGLEKTVEWYLANQHWLVQ
                     VTSGAYKQYYEQQYATN"
     gene            886..1638
                     /locus_tag="PLL28_09010"
     CDS             886..1638
                     /locus_tag="PLL28_09010"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphatase"
                     /protein_id="HNF69501.1"
                     /translation="MSSVFGRFFSGNKTEHTDFSALGADMHAHIIPGIDDGSQDVATS
                     IAMIKGLQELGFSKFISTPHIMPGVFNNDEESIRAATELLNQSAPKETGTTVQYAAEY
                     YIDDDFPQMVNSGKPLLTFGDQYVLVEANMMSRTRNMEEALFELRLRGLRPILAHVER
                     YPYMYEHHRLDQYEKLRDADVLLQVNLRSFTGNYGEIQKRIARHLADAGMISFLGTDL
                     HNPAQLAIIRESMLDQHVQNLLKSGDLLNNTL"
     gene            1713..2288
                     /gene="rfbC"
                     /locus_tag="PLL28_09015"
     CDS             1713..2288
                     /gene="rfbC"
                     /locus_tag="PLL28_09015"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNF69502.1"
                     /translation="MPFIETPISGLYVYEPKVFEDARGYFYESFNAQVFGEKGINTNF
                     VQDNQSRSSYGVLRGLHYQLAPHAQAKLVRVISGEVYDVAVDIRKGSPTFGQHFGVIL
                     SAENKKQLYIPRGFAHGFVVLSETAEFFYKCDNFYNKEHDAGIRFDDATINIDWKIPH
                     SDIQVSEKDANLPNLQDAKNNFIYGNESPIA"
     gene            2285..3925
                     /gene="rfbD"
                     /locus_tag="PLL28_09020"
     CDS             2285..3925
                     /gene="rfbD"
                     /locus_tag="PLL28_09020"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNF69503.1"
                     /translation="MNILITGSNGQLGNELRVIAQRTPQHTYTFVDVDRLDITDSLAV
                     DRFFAEGNYAVCINCAAYTAVDKAETDRDLCYRINVTGVEHLANACQKHHAVFFHIST
                     DFVFDGTGTQPYRIDHHVQPLSVYGQTKAEGEYRALSLNEKTILIRTSWVYSTFGANF
                     VKTMIRLGKERAELGVVDDQIGCPTYARDLAEVIFAFIPKLGDVPYGIYHFSNSGFTS
                     WAGFAEKIMQLYGLNCRVNRITTEQYPTAATRPEYSVLDCSKIIETLHISIPTWESAL
                     QDCIQQLKQQEMLQQINVQDVIAIAEAAGKEIMHIYATDDFAVTDKSDNSPLTKADKA
                     SNAVIVAALQKQYPQIPIISEENKLLAYDERKGWDTLWLVDPLDGTKEFIKKNGEFTV
                     NIALIQNGKPVMGVVGIPARNHMYYAVEGKGAYKVDEHGNTTKLQIAEPRTDTIALIG
                     SRSHPSPEFDAYLKDMESKYAQVDFVPAGSSLKFCLVAEGKADVYPRLGPTMEWDTAA
                     GHAVVLEAGARVKVYGSTDDLSYNKENLLNPFFIVEKA"
     gene            complement(4168..5076)
                     /locus_tag="PLL28_09025"
     CDS             complement(4168..5076)
                     /locus_tag="PLL28_09025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69504.1"
                     /translation="MKIIKTLDINAYASIAAYFLGIPNSGVSQVVYVDLNPDITVDEA
                     AFPFGLDMNSDEIIDFEFLHSAFSFYSAPFNSYRYRIDMLVKPLNSQNAISGSYHYHG
                     TFYGGAWWYYPYALLVNDTINESGIWQVGSAQILGLITIDSDDSFIHYAAAADWFNSE
                     ISETTNRFIGVRFIDIENSIHYGWVRCDLINSGNTLIIKDYAYNATNDYGLYAGTEIS
                     INSSIAKDEWNIFTNNNILNIHYNGIDFEISKVSIYSSNGELMYNAELSMNFTPIDVS
                     ELEHGIYFVVVEDNSKRVTKELFLGF"
     gene            complement(5087..6943)
                     /locus_tag="PLL28_09030"
     CDS             complement(5087..6943)
                     /locus_tag="PLL28_09030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69505.1"
                     /translation="MRAEGVNVVGIEMGNECYLEFFCDVLHFYSFEDYWQFINGTNLL
                     GNDDILYNDPSPSVHTDMIEDHDFITAFKTASTSNNYNYKVGIVGMPLEEGFVFNSPQ
                     ETLYGERVSCNDATIWNPAIYSKYGEHVGTSSKYKFDAVIMHTYYTSDHWRDIVFDNF
                     NPVTSCTSSSDLWKYDIYDSRLQATFNGIIGIGDLPGNFKDFLVNGKDDGLGFASAID
                     RMSYYFKFTEAEPNPKDLWITEWNLKDNYRNGTKLEQTECEIYSNTFAHSFLFYQWWL
                     KQLKVNFDPDYRENFLTYSTVQNYINGTSINLVSVADEQEEDYYSVAYGGADCLQRNY
                     HVRRTTYFATYLLSPIFNEKLKYLRTSNTLPFFPNNSGNIQPTVFIDVTGQYLYIYYT
                     NTKNSIQNYRIAQGTLKQLYPDATSISFDEEGTTNTYILAQQLYSSSGKVTLNKYDKD
                     WEVGDPESEFQGLNACYENYDHEMEIMPTTESGNFPTIHNFGNYPQCSGGNYLTLHNC
                     FTAQPYSIGYFKGHFTPMYPLKLNAANLSELVVYPNPANSKIRVAMTDINTAGVETAK
                     IQIFNIQGEIVFVGLFENYNEYDISTLPSGLFQIIITDFYGNFLKSSFVKVE"
     gene            complement(7046..8086)
                     /locus_tag="PLL28_09035"
     CDS             complement(7046..8086)
                     /locus_tag="PLL28_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69506.1"
                     /translation="MHPENKIKMLIKSASRLGSCLIAIISVSLIAHNAADGNNFSIIN
                     IYSNNYHQLPDSLHEDSIETIEGLFDDTITAVEIYVTGDTVGYVIDGLDSIIYTQGDT
                     SGFFIGSGNSESFYVGDADTSTYFTLLNDSLIEIEYADSTSYYVSNEAGAYLIPKGEE
                     IAYPLYKLDTLIEGRTNSGVEIVEISINQNLNDILVSLGHTAKNVNPGLFGVHIGGMF
                     DNLTTPNEVSSEYAWQWLVDLAPTTLRFPQGSGSKFMHLLHYADGSDVTGYGYDIIEI
                     ARYFDYIDGESPFDYTDLSASDINSIQTAVDPSWIPLEYREDFNAYHDKWENQNCEDV
                     DTKFIDDFIALV"
     gene            8636..11644
                     /locus_tag="PLL28_09040"
     CDS             8636..11644
                     /locus_tag="PLL28_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69507.1"
                     /translation="MKKLALQLCLCLGMYQAFAQQAPIPPVDTLAPADPMENPRVQGI
                     QKRKSLQANSIINNVALTNIGPTIMSGRVVDIDVNPKDATKFFVAYASGGLWYTDNNG
                     QSFRPVFDHEDVMTIGDFAVDWSNNIIWVGTGEVNSSRSSYAGIGVYKSEDFGKTWIY
                     CGLPESQHIGRIVINPRDPKMVYVAVLGHLYSANPERGVYKTIDGGLTWKQTLYIDDN
                     TGAVDLLMDPNVPDFLYVTTWQRSRRAHNFTEAGPGSAIYKSVTAGETWELLSKPGSG
                     FPTGPDVGRIGITVAPAHNNIVYAVVDNQGYREMDPSKRDREYDGTSFQMDSLKHMTK
                     EKFAALDSIMLVKFLQDKEFPKEYTAGNLKAKVASGDFAATVLYDYLEDGGYVFNRPI
                     KGCEVYRSDDGGTSWYKTHDGDLDGAFFTYGYYFGNIRVSAKNDNKILVSGFNALLSE
                     DGGKTFKNIDGPNVHPDHHAAWFDPANDKHIIVGNDGGVNITYDNGANWFLANTPAVG
                     QFYSVTVDNAEPYNVYGGLQDNGVWTGPSDFVQSNDWLANGKNPYTALYGGDGMQVQV
                     DTRNNNTVYTGYQFGYYARVDKTTGREELSIRPKQSFGEAPLRFNWQTPILLSKHNQD
                     ILYYGANKLYRSMDKGNNLQALSGDLTNGKKEGDVPFGTITTIAESPKRFGLLYCGTD
                     DGNIWVSKDAGFSWNKINVFIKKELPPVKSKTPPPVFNAIPEGMWVSRVEASHFNEGT
                     VYASLNGYRWDNFTPYLFVSTDFGATWDQIGNDLPSEPINVVREDPKNKNILYVGTDN
                     GLYVSINGGRTFMAWSGSLPRVAVHDIAIQERDNDIIVATHGRSLFKADLEQIQALPD
                     IQTKEIYAFEVEDMPYDKNAGKRYTNFDPAQETSYQLAYYVQTAGQTTISIATPNGAI
                     VREWTDESEAGLNYVSYDLTLEPAARSAYEDYLNGLPAKGVREVVVLKEADNGKLYLR
                     PGKYIVRFTSEKLVPAQQSFEITDNENGAE"
     gene            11735..11977
                     /gene="rpmB"
                     /locus_tag="PLL28_09045"
     CDS             11735..11977
                     /gene="rpmB"
                     /locus_tag="PLL28_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNF69508.1"
                     /translation="MARVCELTGKKTVSGNNVSHSNRKTRRKFYPNLQVKRFYIPEED
                     RWVTLKIAASTLRTINKKGISAYLKELRSEGKIVNI"
     gene            11989..12177
                     /gene="rpmG"
                     /locus_tag="PLL28_09050"
     CDS             11989..12177
                     /gene="rpmG"
                     /locus_tag="PLL28_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNF69509.1"
                     /translation="MAKKSKGNRIQVILECTEQKNTEVAGMSRYITTKSRRNTPDRIE
                     LKKYNPFLKKVTVHKEIK"
     gene            12194..12376
                     /locus_tag="PLL28_09055"
     CDS             12194..12376
                     /locus_tag="PLL28_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 family protein"
                     /protein_id="HNF69510.1"
                     /translation="MGKISKNARVSGGAKAKAVGGGKEMVKVIVTEKSPKTGAYIFKE
                     RIVHKDKVQDVLKGNA"
BASE COUNT         3842 a         2557 c         2611 g         3502 t
ORIGIN      
        1 taaaaggtga tatcacagat gctggattta tcacttcgct ttttgaagaa catcgctttg
       61 atatggttgt gcatcttgcc gctgaaagcc atgtggatcg aagtattgca aaccctattg
      121 aatttgtaat taccaatgtg gttggtacat gtgtattatt aaatgctgcg aggaatattt
      181 ggaaggatga ttattccgga aaacgattct atcatatctc taccgatgaa gtatatggaa
      241 cattaggaga gtccggctta tttacggaaa caacacctta cgatccgcat agtccgtatt
      301 ctgccagcaa agcatccagc gaccattttg ttcgcgctta ccacgatact tatggcatgc
      361 cggtggtaat ctcaaattgc agcaataatt acggatcatt tcagtttcct gaaaaattaa
      421 ttccgctagc cataaataac attaaaaaca tgaagcctat tcctgtttat ggcaaaggcg
      481 aaaatgtgcg cgattggctg tgggtggaag atcatgctcg cgccattgat gtgattctgc
      541 acaacggaaa aaatggagat acctacaata tcggcggatt taatgaatgg aaaaatatcg
      601 acctcattca tttactttgt cgcattatgg ataataaact gcaacgcgaa ccggggacat
      661 ctgcaaagct catcacttat gtaactgatc gtcccggtca tgatatgcgc tatgccatcg
      721 atgcgcaaaa attaaatcgc gagttgggat ggaaaccttc tgttgatttc gaacaaggtt
      781 tggaaaaaac ggtagaatgg tatcttgcga atcagcattg gttagtgcag gttacaagcg
      841 gtgcctataa acaatattac gagcaacagt acgcaacaaa ttaatatgag ctcagtgttt
      901 ggcagattct tctcagggaa taaaaccgaa cacacggatt tcagtgcgct gggagcagat
      961 atgcatgcac atatcattcc cggaatcgac gatggatcac aagacgtggc aacttcaatt
     1021 gccatgataa aaggtttaca ggaattggga ttctctaaat tcattagcac acctcatatt
     1081 atgcccggtg tgtttaataa tgatgaagaa agtattcgtg cggcaactga attactcaat
     1141 caatctgcac ctaaggaaac gggtacaaca gttcaatatg cggcagaata ttacatcgat
     1201 gatgattttc cgcaaatggt aaacagtggt aaaccactgc tgactttcgg cgatcaatat
     1261 gtgctggtgg aagctaatat gatgtcgcgc acacgcaaca tggaagaggc actgtttgaa
     1321 ttgcgcttgc gcggattgcg tccgatactg gcgcatgtcg agcgatatcc atacatgtat
     1381 gagcatcatc gattagatca atatgaaaaa ttgcgtgatg ctgatgtgtt gctgcaagtg
     1441 aacctccgtt catttaccgg taattatggc gagatacaaa aacggatagc ccgccatctg
     1501 gccgatgcag gaatgatcag ctttctggga accgatctgc ataatccggc tcaattggcc
     1561 attatccggg aatccatgtt ggatcagcac gtgcaaaacc tgctgaaaag cggcgatttg
     1621 ctcaataata ccctctaaat tgtctacaac cattccacat actacccgtt ttcgggcaga
     1681 acggggtatt tttgcagcct gattgaaaat tgatgccttt tattgaaaca cctataagcg
     1741 gactctatgt atatgaacca aaggtatttg aagatgcccg cggttacttt tatgaatcat
     1801 ttaatgcgca agtatttggt gaaaagggca tcaataccaa ttttgtgcaa gacaatcaga
     1861 gtcgctcgtc gtatggcgtg ctgcgcggat tgcactatca gctggcaccg catgcacagg
     1921 caaaactggt gcgcgtaatc agcggtgagg tttatgatgt ggctgttgat atccgcaaag
     1981 gctcaccaac attcggtcag cattttggag tgatattatc tgcggaaaac aaaaagcaat
     2041 tatatattcc aagaggattt gcccatgggt ttgtagtgct cagtgaaaca gctgaattct
     2101 tttataagtg tgataatttc tataacaagg aacacgatgc cggtatccga ttcgacgatg
     2161 ccacgattaa tatagattgg aaaataccac atagcgatat tcaagtgagt gagaaagatg
     2221 caaatcttcc gaacttgcag gatgctaaaa acaattttat ttacggaaat gaatctccta
     2281 ttgcatgaac atactgatta caggatcgaa cggacaattg gggaatgaac tgcgtgtaat
     2341 agcacaacgc acaccgcagc atacatacac ctttgtagat gtagatcgat tggatattac
     2401 ggacagcctt gcagtagatc gtttttttgc ggaagggaat tatgctgttt gtattaattg
     2461 cgcagcgtat actgcggttg acaaagcaga aactgatcgc gatctctgct accgtatcaa
     2521 tgtgaccggt gtggaacatc tggctaacgc ctgccagaaa caccatgctg ttttctttca
     2581 tatcagcacc gattttgtat ttgatggcac tggaacgcaa ccgtatagga tcgatcatca
     2641 tgtgcagcca ttatcggtat atggacaaac aaaggccgaa ggtgaataca gagcattgtc
     2701 gctgaatgaa aaaactattc tcattcgcac gagctgggtg tatagtactt tcggagccaa
     2761 ttttgtgaaa accatgatac gccttggcaa agaacgcgca gaattgggcg tggtagatga
     2821 tcagatcggc tgtccgacct atgcgcgcga cctggcagaa gtgatttttg catttattcc
     2881 gaaattggga gatgtgccat atggcattta tcatttctcg aacagcggct tcacctcttg
     2941 ggccggattc gcggaaaaga tcatgcaatt gtacggactg aattgtcgcg tgaacaggat
     3001 caccaccgag cagtatccca ccgcagcaac acgtccagaa tactcggtgc tcgattgctc
     3061 gaaaatcatc gaaacactgc atatcagcat accaacctgg gaaagtgcat tgcaggattg
     3121 tatacaacaa ttaaaacaac aggaaatgtt acaacagata aacgttcagg atgtgatcgc
     3181 tatcgccgaa gcggcaggta aagagatcat gcacatttat gccaccgacg atttcgcggt
     3241 tacggataaa agcgataatt caccattgac aaaagccgac aaagcgtcta acgcagtgat
     3301 cgttgctgca ttgcaaaagc aatatccgca gatacccatc atcagcgagg aaaataaatt
     3361 actcgcctac gatgaacgca aaggttggga taccctctgg ttggtagacc cattggatgg
     3421 cacgaaagaa ttcatcaaaa agaatggtga gttcactgtg aatatcgccc tcatccaaaa
     3481 tggtaaaccg gtaatgggcg ttgttggcat ccctgcccgc aaccacatgt attatgccgt
     3541 tgaaggaaaa ggtgcgtata aagtggacga gcatggtaat actactaaat tgcaaatagc
     3601 agaaccaaga acagatacca tagcacttat cggctcacgt tcacatccgt cgcctgaatt
     3661 tgatgcctat ctgaaagata tggaatcgaa atatgcgcag gtcgattttg ttcctgccgg
     3721 ttcatctctg aaattctgcc tcgtagccga aggcaaagcc gatgtttatc cacgcctcgg
     3781 ccccaccatg gagtgggata ccgccgcagg acacgccgta gtgctcgaag ccggcgcccg
     3841 cgtaaaagtt tacggcagca ccgacgatct tagttataac aaagagaatt tgctgaatcc
     3901 tttctttatt gtggagaagg cgtaggttgt ctctgatgat atgagatccc atcagccggc
     3961 ttgtgcgggt tgatggggtt gtatgcatta aaccgactag cttgccggta gcgagtgact
     4021 tgctcccacc caaaatccgt tcgtagctat agtcattccg tttgtctcct ttcattccac
     4081 ttaacggcga gcatatttaa tatgaataaa tttgacctgc cgtgaatgtc aataaagaca
     4141 atatattaaa ttaaatgtga gctatagtta aaaacctaaa aacaactctt ttgtcactct
     4201 ttttgaatta tcctcaacca caacaaaata aattccatgc tcaagctcag acacatctat
     4261 tggtgtgaaa ttcattgata attccgcatt gtacattaat tctccatttg aactatatat
     4321 acttactttt gaaatttcga aatcgattcc attgtaatga atgtttagga tattattgtt
     4381 tgtaaatata ttccactcgt ctttcgctat cgagctattt atactgattt cagttccagc
     4441 gtataaacca taatcattcg ttgcattgta agcataatct tttataatca aggtgtttcc
     4501 ggaattgatc aaatcacatc gcacccaacc ataatgaatc gaattctcta tatcaataaa
     4561 tctaactcca atgaacctat tggttgtttc cgaaatttca gaattaaacc aatcagccgc
     4621 cgcggcataa tgaataaagc tgtcatcaga atcaattgtg attagaccta gaatctgtgc
     4681 tgagccaact tgccatatcc cagattcatt aatcgtatca tttaccaata atgcataagg
     4741 ataataccac catgctccgc cataaaaagt gccatgatag tggtatgatc cactgatagc
     4801 gttttgggaa tttaaaggtt taaccaacat atcaattcta tatcgatatg aattaaaggg
     4861 agcgctataa aacgaaaagg ccgaatgaag gaactcgaaa tcaattattt catctgaatt
     4921 catatccaat ccaaatggga aagctgcttc gtctactgta atgtcaggat ttagatcaac
     4981 ataaacaacc tgcgatactc cagaattcgg aatccctaga aaatatgccg ctatggatgc
     5041 ataagcgtta atgtccaaag tttttattat tttcatttaa aaatctctat tctactttaa
     5101 cgaaacttga tttcagaaaa ttgccataaa aatcagttat gataatttga aataacccag
     5161 aagggagtgt ggaaatatca tactcattat aattctcaaa tagcccaaca aagactatct
     5221 cgccctgaat attaaatatt tgaattttgg ctgtttcaac gccagcagta ttaatatccg
     5281 tcatggcgac acgaatctta gaattcgctg gatttgggta aactacaagt tcacttaaat
     5341 tcgcggcatt taacttcaaa ggatacatag gtgtaaagtg ccccttaaag tagccaattg
     5401 aatatggttg cgccgtaaaa cagttgtgaa gtgttaaata attaccgccg gaacattgtg
     5461 gatagttgcc aaaattgtga atcgttggga agttgccaga ttccgttgtt ggcattattt
     5521 ccatttcatg atcgtagttc tcataacaag cattcaatcc ttgaaattct gattctggat
     5581 caccaacctc ccaatcttta tcatatttat tcaatgtaac ttttccagag gatgaataca
     5641 gctgttgtgc caagatatat gtatttgttg tgccttcttc atcaaagctt atgctggttg
     5701 catctgggta tagctgtttc aaggtgcctt gggcaattct ataattctga atcgaattct
     5761 tagtatttgt ataataaata tacaagtatt gtccagttac atctataaaa acagttggtt
     5821 ggatatttcc gctattattt ggaaaaaagg gtaacgtgtt tgaagttcgc aaatatttaa
     5881 gcttttcatt gaaaattggg ctcaaaaggt aggtagcaaa gtacgttgtc cttctgacat
     5941 ggtaattgcg ctggaggcaa tccgcgccgc cataggcgac actatagtag tcttcttctt
     6001 gttcatctgc aactgaaacc aaattaattg atgttccatt aatatagttt tggacagtag
     6061 aataagttag aaagttttct cgataatcag ggtcaaaatt aactttgagc tgtttaagcc
     6121 accactgata aaacaaaaat gaatgtgcaa atgtgttact atatatttcg cattctgttt
     6181 gctcgagctt tgtaccattt ctataattgt cttttaagtt ccattccgta atccaaaggt
     6241 cttttggatt gggttcagcc tcagtaaact taaaataata actcatccta tctatcgctg
     6301 aagcaaatcc tagcccatcg tctttgccgt ttactaagaa atccttgaaa ttaccaggta
     6361 ggtcgccaat gccaataata ccattgaatg tagcttgcaa acgactatcg tatatgtcat
     6421 atttccacaa atctgaagag cttgtgcaag aagtaaccgg attaaagtta tcaaaaacaa
     6481 tatccctcca atggtcacta gtataatatg tgtgcattat aacggcatca aatttatatt
     6541 ttgaacttgt gccaacatgc tctccatatt tagaataaat tgctggattc caaattgttg
     6601 cgtcattgca acttactcgt tcaccatata atgtctcctg tggggaatta aaaacaaatc
     6661 cttcttcaag aggcatacca acaataccaa ccttataatt ataattgttg ctagttgaag
     6721 ccgttttaaa agctgttata aaatcatgat cctcaatcat atcagtatga accgaaggac
     6781 tggggtcatt gtaaagaatg tcatcatttc ccagtaaatt tgttccatta ataaactgcc
     6841 aatagtcttc aaaagaataa aaatgcagca catcacaaaa aaattctaga taacattcat
     6901 tgcccatttc aattccaaca acattgactc cttctgcgcg caaataatct gttatcgctc
     6961 tgcactcagt ggcaggttcg cttataatat ttaagtcgag aatgactttt gtttttggcc
     7021 ttcctgggtc aacggcatct attgattaaa caagggcgat aaaatcatcg ataaatttag
     7081 tatcgacatc ttcacaattc tgattttccc atttatcatg atatgcatta aaatcttccc
     7141 gatattctaa tggtatccat gaggggtcaa ctgcagtttg gattgaatta atatcagaag
     7201 cactgagatc cgtgtaatca aaaggagact ccccatcaat ataatcaaaa tatcgagcaa
     7261 tttcgataat gtcatagcca tagccagtta catctgaccc atctgcatag tgaagcaagt
     7321 gcataaattt acttcctgat ccttgaggaa atctcaaagt agttggtgcc aaatcaacga
     7381 gccattgcca tgcgtattca ctagatactt cattcggagt tgtgagatta tcaaacattc
     7441 caccgatatg gactccaaac aatccgggat tgacgttttt cgcagtatgg cctaaactta
     7501 ctaaaatatc atttaaattt tgattaatgg atatttcaac aatttctaca ccggaatttg
     7561 tccttccctc aatcaatgtg tctaatttgt ataatggata agcgatttcc tcacctttag
     7621 gtatcaaata cgctccagcc tcgtttgaaa cgtagtatga agttgaatct gcatattcta
     7681 tttcaataag tgaatcattc aataaggtaa aatatgtgga ggtgtctgcg tcaccgacgt
     7741 aaaaggattc cgaattgcca gaaccaatga aaaagcctga agtgtcaccc tgagtgtaaa
     7801 taattgaatc aagaccatcg ataacgtagc caacagtgtc gccagttaca tatatttcta
     7861 ccgccgttat cgtgtcatca aataagcctt caatagtctc aattgaatct tcatgcaaag
     7921 agtctgggag ttgatgataa ttgttcgaat aaatatttat aattgagaaa ttatttccat
     7981 cggccgcatt atgagcaatt agacttacgg aaataatagc aattaagcaa gatcctaatc
     8041 gggacgcaga tttaattagc atttttattt tgttttcggg gtgcataatg tttgttttgt
     8101 ttgggttttt taaatgttgt tttaacgagc aatcacatag acaaagcaaa ttagggcgat
     8161 ttctttagaa ttctgaagcc ttattaaaat ttgttttata aatgtaataa atagttttct
     8221 atttcggtaa ttcagttttc cttttagggt aaaagaattg ttctttggat tgcaaaatct
     8281 gaaatttgct tattgatgag ataatattta cgagtttagg ttcagaatta ttcccgtttt
     8341 ctggaaattg tgagataatc cacaaaccca gccaaagtct tgccctttaa tttaacaagc
     8401 taaaagctca tttagtgaca tgacaaaagt gtttgattct gtcgaattca ttaaggtagt
     8461 ttgaggttgt attttatcct aaaaaacata ttgtttgctg cgttaggcct ttataatgca
     8521 atgtttgaga ggtttgtaaa gatgtaaaaa atcgcaacta tgtgacatca ttttaatccc
     8581 ttgcagatac ccattcccct gataatccgt attttagagc catcatcagc accgtatgaa
     8641 gaaactagcg ctacaacttt gcctttgttt gggcatgtat caagcctttg cccagcaggc
     8701 accaattcct cctgtagata ccctcgcacc ggcagatcct atggaaaacc cgcgcgtgca
     8761 gggtatccaa aaacgcaaat cactacaagc caattccatt atcaataacg ttgcccttac
     8821 caatatcggc cctaccatca tgagcggtcg tgtggtggat atcgatgtga accccaagga
     8881 tgccaccaaa ttcttcgtgg cctatgccag cggcggactg tggtataccg ataacaacgg
     8941 acaatctttc agaccggttt ttgatcatga ggatgtaatg accatcggcg attttgcagt
     9001 cgactggagt aataacatca tctgggttgg caccggcgaa gtcaacagct cgcgctccag
     9061 ctatgcaggt atcggtgttt acaaatctga ggatttcggt aagacctgga tctattgcgg
     9121 actccctgaa tcacagcata tcggccgtat cgtcatcaac ccccgcgatc cgaaaatggt
     9181 gtatgtggca gtgctcggtc acctctattc tgccaatcca gagcgaggcg tctacaaaac
     9241 catagatggc ggacttacct ggaaacaaac actgtatatc gatgataata ccggcgccgt
     9301 tgacctgctg atggatccca acgtgcccga ttttctgtat gtcaccacct ggcaacgctc
     9361 acgtcgcgct cataatttta cggaagccgg acccggctct gcaatctata aatcagtaac
     9421 cgcaggcgaa acatgggaac ttctatcaaa accgggttcc ggatttccta caggtcctga
     9481 tgtaggtcgt atcggcatca cagtagcgcc tgcacataac aatattgttt atgccgtagt
     9541 agataatcag ggatatcgcg aaatggaccc ttccaagcgc gatagagaat atgatggcac
     9601 ctcatttcag atggacagtc tgaaacacat gaccaaagaa aaattcgcag cactcgacag
     9661 tattatgctg gtgaaatttt tacaggataa agaattccct aaagaatata ccgccggcaa
     9721 tcttaaagca aaagttgcca gtggtgattt tgcagcgacc gtattgtatg attatctcga
     9781 agatggcggt tatgtattta atcgccccat caaaggttgt gaagtatatc gcagcgacga
     9841 tggcggaaca tcttggtata aaacacacga cggtgatctg gatggcgcct tctttaccta
     9901 tggatattat ttcggcaaca tccgcgtcag cgcaaaaaat gataacaaaa tattagtgag
     9961 cggatttaat gcattgttga gtgaggatgg cggtaaaacg tttaaaaata tcgatggtcc
    10021 caatgtgcat cccgatcatc atgcagcatg gttcgatccg gcaaatgata aacatatcat
    10081 cgttggaaat gatggtggtg taaacatcac ctacgacaat ggcgcaaact ggttcctcgc
    10141 aaatactcct gcagtaggtc agttctattc cgttaccgta gacaatgctg agccatacaa
    10201 tgtatatggc ggcttgcagg ataatggtgt gtggacaggc ccttctgatt tcgtgcagag
    10261 caacgactgg cttgcgaatg gaaagaatcc atatactgct ctttatggcg gtgatggcat
    10321 gcaggtgcag gtggatacca gaaataataa taccgtttac accggatatc aatttggtta
    10381 ttatgcgcgc gtcgataaaa ctaccggtcg cgaagaattg agtattcgcc ctaaacaatc
    10441 attcggcgaa gcaccgttgc gatttaattg gcagacaccg attttattga gcaaacacaa
    10501 tcaggatatt ttatactatg gtgcgaataa attatatcgc tccatggata aaggaaataa
    10561 tttgcaagca ctgagtggcg atcttacaaa tggtaaaaaa gaaggagatg tgccattcgg
    10621 aacaattact accatagccg aatcaccaaa gcgatttggg ttgttgtatt gtggaaccga
    10681 tgacggcaat atctgggtga gcaaggatgc cggattttca tggaataaaa taaatgtatt
    10741 catcaaaaaa gaattacctc cggtaaaaag caaaacgcct cctcctgttt tcaacgccat
    10801 tcctgaaggt atgtgggtga gcagggtaga agcttcacat tttaatgaag gaacagtgta
    10861 cgcctcgttg aatggttatc gctgggataa tttcacaccg tatctgtttg tgagcactga
    10921 tttcggtgct acctgggatc aaataggcaa cgacctgcca tcagagccaa ttaatgtagt
    10981 gagggaagac cctaaaaata aaaacatact ttacgtaggc accgacaacg gtttatatgt
    11041 atcgataaat ggtggtcgca catttatggc atggagcggc agccttcctc gtgttgcggt
    11101 gcatgacatc gccattcagg agcgcgacaa tgatatcatt gttgccacac acggaaggag
    11161 cttattcaag gccgatttgg agcagattca ggcacttccg gacatccaaa ctaaagagat
    11221 ctatgctttt gaggtagaag atatgccata cgataagaat gcgggtaagc gctataccaa
    11281 cttcgatccg gcgcaggaaa caagttatca actggcttat tacgtgcaga ctgccggtca
    11341 aaccaccatc agcattgcca ctcccaatgg ggctatcgtg cgcgagtgga ccgatgagag
    11401 cgaggccgga ctgaattacg tgagctatga ccttaccctg gaacccgctg ccagaagcgc
    11461 ttatgaggat tacctgaacg gattgcctgc caagggcgtt cgtgaagtgg tggtgctcaa
    11521 agaagctgat aacggtaagc tgtacctgcg tccgggcaag tacatcgtgc gttttacctc
    11581 cgaaaaactg gtgcctgcgc aacagtcgtt cgagatcacc gataacgaaa acggggcgga
    11641 ataagcttaa aaaacgtcaa atccacagtt tccacatgat tttttggcgt tttcgtcaaa
    11701 aagtccgata tttgcactct cattttaaaa agtcatggca agagtttgtg aattaactgg
    11761 caagaaaacg gtaagcggga acaacgtatc ccactcaaac cgcaagaccc gccgcaagtt
    11821 ttacccgaat ttgcaggtaa aacgcttcta tattcctgag gaagatcgtt gggtaacatt
    11881 gaagattgct gccagcacgc tgcgcaccat caacaaaaaa ggcatctccg catatttgaa
    11941 agaattgcgt agcgaaggca aaatcgtaaa catttaaatt aagctacaat ggcaaagaaa
    12001 agcaaaggca acaggatcca ggtgattctg gaatgtaccg agcagaaaaa tacggaagtg
    12061 gcaggcatga gcaggtatat tactaccaaa agccgccgta atactccgga caggatcgag
    12121 ttgaaaaaat acaacccatt cctgaagaaa gttaccgttc acaaagaaat caaataaacc
    12181 ctggaggtaa gtcatgggaa aaatatctaa aaacgcccgt gtatcgggtg gtgcaaaagc
    12241 aaaggcagtc ggtggtggta aagaaatggt gaaagttatc gttaccgaaa aatcaccaaa
    12301 aacaggtgcc tatatcttta aagaacgcat cgtgcacaaa gacaaagtgc aggatgtatt
    12361 aaaaggcaac gcctgataca aactcaacta atggtaaaag cttctatttc catagaggct
    12421 ttttttgttt atagcaaaca gaccttttgg aacaaaccat cttccgaaaa cattacatca
    12481 tcacagcagt aacaaacaca tgggtctctt cg
//
LOCUS       DAOQHY010000098        12461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2479_length_12461_cov_4.975254, whole genome shotgun sequence.
ACCESSION   DAOQHY010000098 DAOQHY010000000
VERSION     DAOQHY010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12461
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2479_length_12461_cov_4.975254"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1324)
                     /locus_tag="PLL28_09060"
     CDS             complement(<1..1324)
                     /locus_tag="PLL28_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69511.1"
                     /translation="MNRSNVIIISVALALFIGILVFLFASNGETHFNWNPDYQSDSDE
                     PYGTLIFSELTEKYFPGQEREIVTTNKIKDVLNNPGTEHKIYMFIGESVFYSDSTIES
                     LLNFVSSGNEAFFAVDEIPIILSQRVIRGNSDYKIDSTYYIDTFGDTVSIPLEDEYSL
                     VPEDDKLTGSFITTGMTLNYNDSVFRSSNGYRFVFRVKEKPYDFEWRYFMPEYLQGNA
                     FTDNLGTIQSRNFCNFIRMPYGDGYFYFHLTPIAFTNLFMLEEQKVEYASKVLSYFEP
                     GDIIWDQYSMSFKQKKNNDDQEEGPLKFILSQTSLRWAWYILLSGMGIFLIFRTKRMQ
                     QSIPVMEPNENKSLEFVQTIGRMYLLRNKHHYLVTQKMKLFNHFIQERYQLHTAQYDS
                     AFMSKLQMKSEIPIEDIEGIYALANKLEAKHSVTADELILLHNTLDKFY"
     gene            complement(1321..1974)
                     /locus_tag="PLL28_09065"
     CDS             complement(1321..1974)
                     /locus_tag="PLL28_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69512.1"
                     /translation="MRWLLILLLSISWQFSFAADQPVYHPIDKETWKKVTDGVSYYEE
                     EEKVETTPEDIHVPTPAKPISVSPIVQMIGIVLIVALLAFIIWKLFGNIFTGNKKVDA
                     VIINSDNDLDDRPMETDLERYLREALERGDFRMAVRIYYLMLLKALHDGGKIEWRKEK
                     TNRDYLIELYQHPQFHRLNNSTLVFEYVWYGECKVDSNTFSAIKPGFVDLIQKVSQP"
     gene            complement(1971..3881)
                     /locus_tag="PLL28_09070"
     CDS             complement(1971..3881)
                     /locus_tag="PLL28_09070"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HNF69513.1"
                     /translation="MRETSFIDQNKKKWQTFEDNYEREQDPEKVSNLFIQITDDLSYA
                     RTYYPNRSVKIYLNNLAQRVFQNIYKNKVRRRQKFASFWKDELPQKMFEARWQLLFTF
                     ALFMLAFFIGMFSSMHDSNFARFILGDTYVNMTEENIRSGDPMKVYKEMNQVDMFLAI
                     TVNNLQVAFITMIFGIFFGLGTVYFILYNGVMIGVFQYFFIERGLFQESFLTIWVHGA
                     LEVSAIVIAGTAGITLGKGLLFPGTLTRLQSFRVHGMRAVQIFMGIAPIIVLAGINES
                     FLTRYTETPDLIRALLIAVEFGFMIYYFVIYPIRKSRAGFDVKRKSDEIPPNREFSIS
                     LSQVKNNGQVFSDSFLVFRQFMGPIIRTVSLVGILYVAVLFLFVVKFEDVAHNIQIIK
                     AMAIFEAIKDIKSLLMYGNIGSTAENNNGMVLSGGLVLFIANTLSLTIITGMALFIVR
                     SVRDKIFTYSRTAFFKFMLRNGWIILICSIALHCIMLLNLTISRFIFILAVPGIMLII
                     GQIAQRDGAGFAVFSKSGYFFRNIVQVMLLYVPLLLLSIMIVCLSHSLLCFFNVELLA
                     WNVPFSPIIYEAIQDISITLFLIITLFMMIGMIAINMILMYFSINEIESAGALQQRIL
                     TLKKNKKEFSKA"
     gene            4008..4733
                     /locus_tag="PLL28_09075"
     CDS             4008..4733
                     /locus_tag="PLL28_09075"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNF69514.1"
                     /translation="MRTIDILTTQNVTINYELADVKDRIFASILDVIFQSIGLVVLSV
                     VLASPGIMGEQIFLYFITLPVIFFYHLGFEIFMDGRTLGKKLLGIRVVKLTGTVPSIN
                     DYLMRWALRPIDIFFSLGSIAIMLVSSSEKGQRLGDIVANTTVIKTNSTQRIGLADIL
                     NIRTLENYTPTYPGARQFSEEEMLLLKEVMDKSIKYKNTAHDDALVEACYLVKERLGL
                     EKLPSDKHLFVRTLIKDYVAMSR"
     gene            4821..6629
                     /gene="typA"
                     /locus_tag="PLL28_09080"
     CDS             4821..6629
                     /gene="typA"
                     /locus_tag="PLL28_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNF69515.1"
                     /translation="MQKIRNIAIIAHVDHGKTTLVDKIMHHCQLFSKEHNELILDNNE
                     LERERGITIVSKNVSVRYKDVKINIIDTPGHADFGGEVERVLNMADGALVLVDAFEGP
                     MPQTRYVLSKAIALGLKPCVVINKVDKENCKPDEVHEAMFDLMFNLGATEQQLDFPTV
                     YGSAKFGWMSNDWKVQTDNVAALLDMVVEHVPAPVYNEGSLQMMVTSIDYSSYIGRIA
                     IGRVVRGSVKPGQPVTLVKRDGTQVKSRIKDLFVFEGLGKAKAEEVQSGDIAAITGVE
                     GFEIGDTIGDFDNPEALPGIPIDEPTMSMLFRSNDSPFFGREGKFVTSRHLRDRLYRE
                     IEKNLALRIEETNSADSFMVFGRGILHLGILVETMRREGYELQLGQPKVILKHIDGQR
                     CEPIETLMVETTEESAGKVIEAVSQRKGEMVMMSTRGDRTHLEFNIPSRGIIGLRTNI
                     LNLTQGEAIMSHRFKAYEPWKGDIPSRINGVLIVHETGTSIAYALNKLQERGYFFIGP
                     GEEVYQGQIIGENSRDNDMVVNATRTKKLTNMRASGSDDKVNLPPPHRMPLEEAMEYI
                     DEDELVEVTPLNIRLRKIHLLEHERKKAGTMVEVDD"
     gene            6712..7704
                     /locus_tag="PLL28_09085"
     CDS             6712..7704
                     /locus_tag="PLL28_09085"
                     /inference="COORDINATES: protein motif:HMM:NF036967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HNF69516.1"
                     /translation="MKLKDKFVRRLSSKEDKGDEFGFTNRYAEGVQRIIRQDGTFNVK
                     RIGERKLIFHELLNMNWMRFTLFILFYYTVVNLLFATLYLLIDYNGIGMTSDYQVHNN
                     FLVALFFSAQTLTTVGYGSLYPLSGTVSLLAASEALIGLMGFAIFTGLMYGRFANTPH
                     SLRFSKQALIGPYKNGMGIMFRAANARNHNLTELEVKVTLAMVITADGKTSRKFYPLT
                     IDNNRITYFPLNWTMVHHINEDSPLYGMTLQDMSDAEVELLIMIKGFNETSSQEMHAR
                     FSYTHREMVWNAKFKLPYYFDEKGATVFELNKIDDFELLEPVIVTEAVAAAVSE"
     gene            complement(7706..8254)
                     /locus_tag="PLL28_09090"
     CDS             complement(7706..8254)
                     /locus_tag="PLL28_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69517.1"
                     /translation="MQPKYFPSSQIRLILIVCGFAALLAGRATGSMNTLNAVLIIIQL
                     VTLELILRESTIFNTIYYRLLYICSSVIIIGAMMIILHWPYANYVVIMGMSSVAIVYT
                     LRSINKQHKALLDYAKWIWLVLNTIAGILNLLHGQYALMSSYLATGAFFLMMLVFFIS
                     PRTSKGMEQRPPEEDDLPIDQI"
     gene            complement(8264..8734)
                     /locus_tag="PLL28_09095"
     CDS             complement(8264..8734)
                     /locus_tag="PLL28_09095"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HNF69518.1"
                     /translation="MKNKLKEVASIFEQETVSEFKQRIEKLTPVTQHLWGTMTVSQML
                     AHIQQPIMVALGDTKPKQNMFAKLLGGVLRRKYVNREPFTKNLPTASSFVVSDERNFE
                     HEKQDAIQYIERLSALDRSTISGRKHPIFGNLSAEEWDMLLVKHLEHHLRQFGV"
     gene            complement(8759..9292)
                     /locus_tag="PLL28_09100"
     CDS             complement(8759..9292)
                     /locus_tag="PLL28_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69519.1"
                     /translation="MRINTILISAAIALAMISCNKDETPTPDGLMSNGSFENGGSFST
                     DGWSVSNGASDSDVPDGGGSFSLRLNPASLPGEGYADYIIEDLEGSHTFNASCFMRSF
                     DDWPGSITLKKRTADNVVTILATAASSDAGWIEKTISASATFNSGDVLIIHLSAGSTE
                     IPIDSKYVLFDLVTIEE"
     gene            complement(9376..10569)
                     /locus_tag="PLL28_09105"
     CDS             complement(9376..10569)
                     /locus_tag="PLL28_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69520.1"
                     /translation="MIPEIRSAFNAAFTEEKYAAFTLACQERFNKKIEFKICETPVFV
                     SAEFSRSVKAAGEAIIDQLTDQAFLRHTDAAIPDMWRVPGEEGKPVFLALDFGICVDD
                     AGNLIPQLIEMQGFASLYAWQHELGRQYRQFYPIPEEVHHLFNGLDDIAYRKLLRDTI
                     IGDHDPREVVILEIQPDHQKTWIDFYLTREMLGIQPICISDVIVEGTQLFYILNGEKT
                     CIKRIYNRLIFDELAQRNDLSIQWNMTTPADVEWVCHPNWFFRISKYTMPFLKHASVP
                     QTLFLDQVKEIPADLQNWVLKPLFSFAGHGVIFNVTEADIEHAMPQAGNFILQRKVQY
                     AQVVQTPDEPAKAELRLMYLWLPGTDRPTLVTNLARLSKGVMIGVDYNRNKTWVGGTV
                     AYYAD"
     gene            complement(10609..11433)
                     /locus_tag="PLL28_09110"
     CDS             complement(10609..11433)
                     /locus_tag="PLL28_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase"
                     /protein_id="HNF69521.1"
                     /translation="MSRERKIKLAILDMYDNAPNEGMRCLKAMVQAVGDKIDWEVFEV
                     RNKDEVPDKNFDIYISSGGPGSPFDGEGKTWEPKFFNLVDQITAHNANGHSRKKYMFA
                     ICHSFQLLSRHFELGEVCKRKGTAFGVFPIHKTEHSKRDRYFRNLPDPYYVVDSRDWQ
                     LIEPDFAKMTALGASIMAMEKKREHVPLPRAVMAIRLSDEMYMTQFHPEADAEGMLRL
                     FAKPEKRETIRNSHGDWKLEEMVRFLSDQDKIPLTHKEVIPNFLRSSIGALTMSDT"
     gene            11591..>12461
                     /locus_tag="PLL28_09115"
     CDS             11591..>12461
                     /locus_tag="PLL28_09115"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNF69522.1"
                     /translation="MKQTTLGAKLVVLLSLCVLSVTRSTAQAPAKPNIVFIMTDDARY
                     DEFRPSGGADWFIAPNIERIANEGINFNRTYAPTPICGPSRASIYTGVYSHQSGTQNN
                     GDDLSETLPLIQNILIDDGYYTGFIGKYGNGFPSPNQFDYWVDIGDQELYKNLWIKVN
                     GTNVFVSGHLTDAFNTYLNAFMDSAAVHNDKPFALFFFPLAPHTPNVPRATDAAMYNA
                     EVKPFPENFYPYDTLYPTYYTDPGSLWVKDTTATVKFIQDRYSCLYGVDENVSKIMLH
                     LDDTHETDSSIIFF"
BASE COUNT         3636 a         2646 c         2521 g         3658 t
ORIGIN      
        1 tataaaattt atcaagtgtg ttatgtaata atatcaattc atcggcagtt accgagtgtt
       61 ttgcttccaa tttatttgcc aatgcatata tgccttcaat atcttcaatc ggaatctccg
      121 atttcatttg cagtttcgac ataaatgcgc tgtcgtattg agcggtatgc aattggtatc
      181 tttcctgtat gaaatgattg aataatttca ttttctgggt taccagataa tgatgtttat
      241 ttctcaacag atacatcctt ccgatagtct gaacaaactc taatgattta ttttcattag
      301 gctccataac aggaatggat tgttgcatcc gctttgtcct gaaaataaga aaaataccca
      361 ttcctgaaag caggatatac catgcccacc gcaggcttgt ttggcttaat atgaatttca
      421 aagggccttc ttcctgatcg tcattatttt ttttctgttt gaaactcatg gaatactgat
      481 cccaaataat atcaccgggc tcaaaataac tcaatacctt agatgcatat tctacttttt
      541 gttcctcgag catgaataag ttggtaaatg cgatcggagt cagatgaaag tagaaatatc
      601 catcgccata gggcatgcgg atgaaattgc agaaattgcg actctgaatg gtgccaagat
      661 tgtcggtaaa tgcattgcct tgcaaatatt ccggcataaa atatcgccat tcgaaatcat
      721 atggtttttc tttcacgcga aatacaaatc gatagccatt cgatgaacgg aaaacagaat
      781 cattgtaatt caaagtcata cctgtagtga taaagctgcc tgtcagctta tcgtcttcag
      841 gcacaaggct atattcgtct tccagcggaa ttgaaacggt gtcgccgaaa gtatctatgt
      901 agtaagtgct gtctattttg tagtcgctgt tacctcgaat aacacgttgc gataaaataa
      961 tgggaatttc atccacggcg aaaaacgctt catttccgga tgaaacgaaa tttaataatg
     1021 attcaatagt gctgtcggaa tagaaaacag attccccaat aaacatgtag attttatgtt
     1081 ctgtgccggg attgttcagc acatccttta ttttattcgt tgtcacaatt tctctttcct
     1141 gacccggaaa atatttttcg gtaagctcag aaaaaattag ggtgccgtat ggttcatcgg
     1201 agtctgattg gtagtcggga ttccaattga aatgcgtctc cccgttagag gcaaacagaa
     1261 acaccaatat tcctataaat agggcaagtg caacgctgat tataatcaca ttcgacctgt
     1321 tcatggctgg ctgacttttt gaatgaggtc gacaaaaccg ggtttaattg cactgaacgt
     1381 gtttgaatct actttacact caccatacca cacatattca aataccagcg ttgaattgtt
     1441 cagtcggtgg aattgtggat gttgatataa ttcgataaga taatctctgt tcgttttttc
     1501 cttgcgccat tcaattttcc cgccatcatg taaagccttc aacaacatga gataataaat
     1561 gcgcacagcc atccgaaaat cacctcgctc caatgcttct cgcaaatagc gttcgaggtc
     1621 agtctccata ggcctgtcgt cgagatcatt atcgctgtta ataataacag catctacttt
     1681 tttatttcct gtgaaaatat taccgaaaag cttccatatg ataaatgcca gtaatgctac
     1741 aatcaacaca atgccgatca tctgcacgat gggactgaca ctgatgggtt tggccggggt
     1801 agggacatga atatcttccg gcgttgtctc aactttttct tcctcttcgt aatagctgac
     1861 accatcggtc acttttttcc atgtttcctt atcaatagga tggtatacag gctgatccgc
     1921 ggcgaatgaa aactgccatg aaattgaaag caataatatc aacaaccacc tcatgcctta
     1981 ctaaattctt ttttgttttt tttcagggtt aaaatgcgtt gttgcaaggc acctgcggat
     2041 tctatttcgt taatgctgaa atacattaaa atcatgttga tggcaatcat gcctatcatc
     2101 ataaacagtg tgataattaa gaataacgta atgctgatat cttgtatcgc ttcatatatg
     2161 atcggactaa atggcacatt ccatgcaagt agttctacat tgaaaaagca taggagagag
     2221 tgcgacaaac atactatcat gatgctcaat aataacagtg gaacgtacag caacatcacc
     2281 tgaacgatat tgcggaagaa atatcctgat ttactgaaaa cggcaaaccc cgctccatca
     2341 cgctgcgcga tttgaccgat gataagcatt atgcccggta ctgcaagtat gaaaatgaat
     2401 cttgaaatgg taagattcaa aagcattatg caatgcaatg cgatgctgca aattaaaata
     2461 atccatccat tgcgaagcat gaatttaaaa aaagctgtcc gactgtaagt aaatatctta
     2521 tcgcgcacac ttcgaacgat aaacaaggcc atgccggtaa tgatggtaag tgataaagta
     2581 ttggcaataa ataacaccag gccgccactc aataccatac cattattatt ttcggccgtg
     2641 ctgccgatgt tgccgtacat taacaatgac ttaatatcct ttatcgcttc aaatattgcc
     2701 atagccttaa tgatctgaat attgtgggcg acatcttcaa atttgacaac aaataaaaac
     2761 aaaacggcaa catacagtat gcctacaagt gaaaccgttc gtataatagg ccccataaat
     2821 tgacggaata ccaaaaatga atcggaaaat acctgaccat tatttttcac ctgtgaaagt
     2881 gaaattgaaa attcgcgatt gggcggtatc tcgtcagatt tgcgttttac atcaaatcct
     2941 gctcttgatt ttctaatcgg gtaaatcacg aaataataaa tcatgaaccc aaattcgaca
     3001 gctattaata aagctctgat taaatccggc gtttctgtat agcgcgtcag aaaactttca
     3061 ttgatgcctg ccaacacaat gattggtgca atacccataa atatctgcac agcgcgcatt
     3121 ccgtgtactc taaaagattg caatcgcgtt aaagtgccag ggaaaagcaa ccctttgcct
     3181 agggtaatgc ctgctgttcc tgcaattact atggcagaga cctccaatgc tccgtgcacc
     3241 caaatggtta aaaatgattc ctgaaaaagt ccgcgttcaa taaagaaata ttgaaatacg
     3301 cctatcatta caccattgta cagaataaaa taaacggtgc ccagcccgaa gaatatgcca
     3361 aatatcatag tgataaatgc tacttgcaga ttattaacag taattgcaag aaacatatct
     3421 acctgattca tttctttgta aaccttcatc gggtctccag aacgaatgtt ttcttcggtc
     3481 atattgacat aggtgtcgcc taaaataaat ctggcgaaat tgctgtcgtg catactgctg
     3541 aacatcccga taaagaatgc cagcataaat aatgcaaaag tgaacagcag ttgccacctg
     3601 gcttcaaaca ttttttgcgg taattcatct ttccagaagg aagcgaattt ctgcctgcga
     3661 cgaactttat tcttgtaaat attttgaaaa acgcgctggg cgagattgtt caggtagatt
     3721 tttaccgaac gattcggata gtaggttcgg gcataggaaa ggtcatctgt tatctgaata
     3781 aacagattgc ttaccttctc cggatcctgc tcacgttcgt aattgtcttc aaacgtttgc
     3841 cacttcttct tgttctgatc tataaaactg gtctcgcgca tgttactgag gatgcagtgg
     3901 taaatataag agatttgctc gtagattgtg aaattcatac ctattgctca ccatttacct
     3961 aacaatgtga gcggatttta aaaaaaatgt atttttgttt tgattacatg cgcaccatcg
     4021 atatccttac tacccaaaat gtaaccatca actacgaact ggctgatgtg aaagaccgca
     4081 tcttcgcgag cattttggat gttatttttc agtcgatagg tttggtggta ctctctgtag
     4141 ttttggcctc gccgggcatc atgggcgaac aaattttcct ttattttata acacttcctg
     4201 ttatattctt ttaccacctt ggctttgaaa tcttcatgga cggtcgtacc cttggtaaaa
     4261 aactccttgg tattcgagtg gtaaaactta ccggaactgt tccctccatc aacgattatc
     4321 tcatgcgctg ggctttgcgt cctattgata ttttcttttc tctgggctcc attgctatta
     4381 tgctggtatc atcctccgag aaaggtcagc gattgggtga tattgtggct aataccactg
     4441 ttattaaaac gaattctacc cagcgcattg gcctggctga tatcctgaac atcagaactc
     4501 ttgaaaatta tacccccacg tatcccggcg ccagacagtt ttctgaggaa gaaatgctgc
     4561 ttttgaaaga ggtgatggat aaaagcatca aatacaagaa caccgcccac gacgatgccc
     4621 tcgtagaagc ctgttatctg gtgaaagagc gcctgggact ggaaaaattg ccttcagata
     4681 agcacctctt cgttcgcacc ctcatcaagg attatgtggc tatgagtcgc taatcatgtt
     4741 aataatatct gaagtgcggc acttattcac cgatttcctg cgttattgcc tgtaaaaacg
     4801 ctacctttgc agccgttttt atgcagaaga tacgcaatat agccatcatc gcccacgtcg
     4861 accatggtaa aactacgctg gtagacaaga tcatgcacca ttgtcagctt ttttcaaaag
     4921 agcacaatga actcattctt gataataatg aactcgaacg cgaacgtggt atcaccatcg
     4981 tgtctaaaaa cgtttctgtt cgttataaag atgtgaaaat caacattata gatacaccgg
     5041 gacacgccga tttcggaggt gaagtggaac gcgttctcaa tatggccgac ggtgctctcg
     5101 tgctcgttga tgcctttgaa ggtccgatgc cgcagacccg ctatgtgctc agcaaagcca
     5161 tcgcgctggg tttgaagcct tgtgtggtga tcaataaagt ggataaagaa aactgtaagc
     5221 ccgatgaagt gcatgaggct atgttcgatt tgatgttcaa cctcggtgca actgaacaac
     5281 agcttgattt cccgactgtt tacgggtcgg ccaagttcgg atggatgagc aacgactgga
     5341 aagtgcaaac cgacaacgtt gctgcgctcc tggatatggt tgttgagcat gttcctgctc
     5401 ctgtttacaa cgagggttct ctgcaaatga tggttaccag tatcgactac tctagttata
     5461 tcggtcgtat tgccatcggt agagtggtga ggggttcggt aaaacccgga caaccggtta
     5521 cgctcgtaaa acgcgacggc acgcaggtga aaagtcgtat caaagatctt tttgtattcg
     5581 aaggacttgg aaaagcgaag gctgaagagg tgcaaagtgg cgatatcgcg gctattaccg
     5641 gtgttgaagg cttcgaaatt ggcgatacta ttggcgattt cgataatcct gaagccttac
     5701 ccggcattcc tatcgatgag cctaccatga gcatgctgtt tagaagtaat gactcaccat
     5761 tcttcggtcg cgaaggtaaa tttgtcacca gccgccacct ccgcgatcgc ctgtatcgcg
     5821 aaatcgagaa aaatcttgcc cttcgcatag aagaaaccaa tagtgccgat tctttcatgg
     5881 tattcggtcg cggtatccta cacctcggaa tcctggtgga aaccatgcgc cgtgaaggtt
     5941 atgaactgca gctcggacaa ccgaaagtta tcctcaaaca tatcgacgga caacgttgtg
     6001 aaccaatcga gaccttaatg gtggaaacca ctgaggaatc ggcaggtaaa gttatcgaag
     6061 ccgttagcca gcgaaaaggt gaaatggtca tgatgtcgac tcgtggcgac cgcacccatc
     6121 ttgaattcaa tatcccatca agaggtataa tcggtttgcg taccaatatc ctgaatttaa
     6181 cacaaggtga ggctatcatg agccatcgct ttaaagccta tgagccgtgg aaaggtgata
     6241 tcccttcgcg tatcaatggt gtgttgatcg tgcatgaaac aggcacctcc attgcctatg
     6301 cattgaataa actgcaagaa cgtggatatt tcttcatcgg ccccggtgag gaagtatacc
     6361 aaggtcagat catcggtgag aacagcaggg ataacgatat ggttgtaaat gccactcgta
     6421 ccaaaaaact gaccaacatg cgcgcaagcg gaagcgatga taaggtgaat ctgccaccac
     6481 ctcaccgcat gcctctggaa gaagccatgg aatatatcga cgaagatgaa ctggtggaag
     6541 taacgccgct caatatccgt ctccgcaaga ttcatttgct ggagcatgag cgtaagaaag
     6601 ccggcaccat ggttgaggtt gatgattaag attgtcagtc gcacatttta ggaactattt
     6661 cttcttgtat ttttgttgtc cttcctctac agggataggg tcaatatcac catgaagtta
     6721 aaagacaaat ttgttcgtcg ccttagcagc aaggaagata agggtgatga attcggattc
     6781 accaatcgct atgccgaagg cgtgcaacgt atcattcgtc aggatggcac atttaatgta
     6841 aaacgaatag gggagagaaa gctcatattt cacgaattgc tgaatatgaa ttggatgcgg
     6901 ttcacgctct ttatattgtt ttattatact gtagttaacc tgttgtttgc aaccttatat
     6961 ctgcttatcg attacaatgg aatcggcatg accagcgatt atcaggtgca taataatttt
     7021 cttgttgcct tgtttttcag cgcacaaaca ttaacaaccg ttggttatgg atcattgtat
     7081 ccattatccg gtacggtcag cttactcgct gcatctgaag cattaatagg attaatggga
     7141 ttcgccattt ttacgggatt gatgtacggt aggtttgcca atacaccaca tagtttaaga
     7201 ttttcgaaac aggcattaat tgggccatat aaaaatggaa tgggaataat gtttcgcgca
     7261 gcaaatgcca gaaatcacaa tctcactgaa ttggaagtaa aggttacatt ggcaatggta
     7321 attactgcgg atggcaaaac aagtagaaaa ttttatccgc ttaccatcga caataatcgc
     7381 attacttatt tcccgctcaa ctggaccatg gtgcatcata ttaatgaaga cagtccatta
     7441 tatggcatga ccttgcagga tatgtcagat gcagaagtgg agttgttgat tatgatcaaa
     7501 ggattcaacg aaacttcatc acaggaaatg cacgcaagat tctcctatac acatcgtgag
     7561 atggtgtgga atgcgaaatt taaattgcct tattatttcg atgagaaagg agctaccgta
     7621 tttgaactca ataaaattga tgattttgaa ttgttggaac ctgtaattgt tactgaagca
     7681 gttgcagcgg ctgtttcaga ataatttaaa tttgatcaat cggcagatca tcttcttccg
     7741 gaggccgctg ttccatgcct tttgaagttc ttggggaaat aaaaaatacc agcatcatta
     7801 agaaaaatgc accggttgcc aaataggatg acatgagtgc atactgcccg tgcaacaggt
     7861 tcaaaatacc tgctatggta ttcaatacaa gccatatcca ttttgcataa tctagtaatg
     7921 ccttgtgttg tttgttgata ctgcgcaaag tatatacaat ggcaacagat gacatgccca
     7981 tgataactac ataattggca taaggccaat gtaaaataat catcattgct ccgatgataa
     8041 ttactgagct gcagatatat agcaatcgat aataaatggt attgaaaatt gtgctctcac
     8101 gtaaaatgag ttccagtgtt accaattgaa taatgattaa aacagcattc aaggtattca
     8161 tgctgccggt tgccctgcct gccagcaaag ctgcaaatcc gcaaacaatg aggattaatc
     8221 tgatttgtga tgaagggaaa tatttcggct gcatgatatt acattacaca ccaaattggc
     8281 gcaagtgatg ttcgagatgt ttcacaagta gcatgtccca ttcttcggca cttaaatttc
     8341 caaatatcgg atgcttgcga ccggaaatgg ttgatctgtc taatgctgat aaacgttcaa
     8401 tatattgaat agcatcttgt ttctcgtgtt caaaatttct ttcatcagat acaacgaaac
     8461 ttgaagcagt gggtaagttc ttggtaaatg gttctctatt tacatatttt ctcctgagaa
     8521 caccacctaa taatttcgca aacatgtttt gcttaggctt ggtgtcaccc aaagctacca
     8581 ttatgggttg ttgaatatgt gccaacatct gcgatactgt catcgtgccc cacaaatgtt
     8641 gtgttacggg tgttaatttt tcaatgcgtt gtttaaattc cgatacggtc tcttgctcaa
     8701 aaatacttgc cacttccttt aatttattct tcaaaaaaat atttcctttt ttatctaact
     8761 attcctcaat cgttaccaaa tcaaataaca catatttact gtcaatcggt atttcagtag
     8821 atcctgcaga taaatgaatg atcaacacat cgccggaatt aaaagttgca cttgccgaaa
     8881 ttgttttttc tatccatccg gcatcactgc ttgcagctgt tgctagaatg gtgacaacat
     8941 tatctgcggt acgttttttt aatgtaatgg atcccggcca atcatcgaat gaacgcataa
     9001 aacatgaggc attgaaagta tgcgaccctt caaggtcttc aatgatataa tctgcatatc
     9061 cttctccggg taaggaagca ggatttagtc ttaaagaaaa gctaccgccg ccatcaggaa
     9121 catcgctgtc tgaagcaccg tttgatactg accatccatc ggtagaaaat gagccaccat
     9181 tctcaaagga gccattcgac atcaatccat caggtgtagg cgtttcatcc ttattacagg
     9241 aaatcattgc taaggctata gctgcgctta tcaggatagt gttaattcgc atatccattt
     9301 ttttctaaag atatacttcc aaacggtaaa tggattggaa caagtgtaag aatgctgtaa
     9361 ttaatttacc gcaactcaat cggcatagta agcaaccgtg ccgccaaccc atgtcttatt
     9421 tctattataa tctacaccaa tcataacccc tttactcaac cttgcaagat tcgtaacgag
     9481 tgtaggacga tcggttcccg gtaaccataa atacatcaat cgcaattcag ctttagcggg
     9541 ttcatcgggg gtctgaacca cctgtgcata ttgcactttg cgttggagga taaaattacc
     9601 tgcctgaggc atcgcatgtt cgatatcggc ttctgtaacg ttgaaaatta ccccatggcc
     9661 ggcaaaagaa aacagtggtt ttaataccca gttttgcaga tcggcaggaa tttctttcac
     9721 ctgatcaaga aataaagttt gcggaacaga agcatgcttt aaaaatggca tcgtgtactt
     9781 gctgatgcgg aaaaaccagt ttgggtgaca aacccattcc acatcggcag gagtggtcat
     9841 attccattgg atagaaagat catttcgctg cgcaagttca tcaaaaatga gcctgttgta
     9901 aattcgctta atgcaggttt tctcgccgtt tagtatgtag aaaagttgtg tgccttcaac
     9961 aatgacatca gaaatgcaaa ttggctgtat tcccaacatt tcgcgtgtca gataaaaatc
    10021 gatccaggtt ttctgatgat cgggctgaat ctcaagtatc accacctccc gcgggtcgtg
    10081 atctccaatt atggtgtctc ttagcaattt tcgataggcg atatcatcta atccattaaa
    10141 aagatgatgc acttcctccg gaatcgggta aaattgcctg tattgtcttc ctaattcatg
    10201 ttgccaggca taaagcgagg caaatccctg catttcaatg agttgtggaa tcagattgcc
    10261 ggcatcatct acacaaattc cgaaatcaag ggctaaaaat acaggtttcc cttcctcacc
    10321 cggaacccgc cacatatcag gtattgcagc atctgtatgt cgcaaaaagg cttgatctgt
    10381 caactgatca atgatcgctt ctcctgcagc cttgaccgat cttgaaaact cagcactcac
    10441 aaataccggt gtttcgcaga ttttaaactc aattttttta ttaaatcgct cctgacaggc
    10501 caatgtaaat gcggcatact tttcttcggt gaatgcggca ttaaaagcac ttcgaatttc
    10561 cggaatcatg gatgggaaaa aattaataag taattgataa tcaaataatt atgtgtccga
    10621 catggttagc gcaccgatgg aagatcgcaa gaaattaggg ataacctctt tatgcgtcag
    10681 cggaatctta tcctggtctg aaagaaagcg taccatctcc tccaatttcc aatctccgtg
    10741 gctattacgg atggtttctc ttttttcagg tttggcgaac aaccggagca taccttctgc
    10801 atcagcttca gggtgaaatt gggtcatata catctcgtcg ctcagcctga ttgccatcac
    10861 cgcacggggt aggggaacat gctctcgctt cttttccatt gccataatac tggctccaag
    10921 cgcggtcatt ttggcaaaat cgggttctat gagctgccaa tcgcgactat ctaccacata
    10981 atatgggtcg ggcaggtttc tgaaatacct gtcccgcttc gaatgttcgg ttttatgaat
    11041 agggaatacc ccaaaagcgg tgccttttcg cttacacact tcgccaagtt caaaatgccg
    11101 gctcagcaat tgaaaagagt gacatatggc gaacatatac ttctttctgc tatgtccatt
    11161 ggcattatgt gctgttattt ggtcaacaag attgaagaat tttggctccc atgtcttgcc
    11221 ttctccatca aatggactgc ccggtccgcc tgaggagata taaatatcaa agttcttatc
    11281 cggaacttcg tctttattcc tcacctcaaa tacttcccag tctattttat cgccaaccgc
    11341 ttgtaccatg gctttcagac aacgcatgcc ctcatttggg gcattgtcgt acatatctaa
    11401 tatcgccagt ttaatcttcc gctcgcggga cataaggtcc aaaattagag tttttaaaaa
    11461 gtgcagatat gtttgttatc caccacttat catcaaaatg tcaatatgcg tcaatttgtt
    11521 tcctaaaggg cagatttaat ggctatattt gctgcatccc atttttgaac caaacagtca
    11581 acacatacca atgaaacaaa cgactcttgg ggctaagctg gtcgtgcttc ttagcctttg
    11641 tgtcttatcg gtaactcgtt caactgctca agctccggct aaacccaata ttgtcttcat
    11701 catgacagat gatgcacgat atgatgaatt tcgtccaagt ggcggagccg actggtttat
    11761 tgcaccaaat attgaacgca ttgcgaatga aggcatcaac ttcaatcgga cgtatgctcc
    11821 aactcccatt tgcggaccga gcagggcatc catttacacc ggtgtgtatt ctcatcagag
    11881 tggcacccaa aacaatggcg atgatctttc agaaacgctc ccattaattc agaatattct
    11941 gatcgatgat ggttattaca ccggattcat cggaaagtat ggaaatggtt ttccgtcgcc
    12001 aaaccaattt gattattggg tggatatcgg cgatcaggag ttatataaaa atctatggat
    12061 taaggtaaat ggtaccaatg tatttgtatc aggacacctt actgacgcat ttaataccta
    12121 cctcaatgcg tttatggact cggccgctgt acataatgat aaaccatttg cattattttt
    12181 ctttccgctt gctccacata caccgaatgt tccgcgagca actgacgcgg cgatgtacaa
    12241 tgctgaggtg aagccttttc ctgaaaattt ttatccttac gatactttat atcccacgta
    12301 ttatactgac ccgggttcac tttgggtaaa ggatacaaca gcaacagtca aatttattca
    12361 ggatcgatac agttgtcttt atggtgttga tgaaaacgtg tctaaaatca tgttacatct
    12421 agacgacacg catgaaacag acagctctat tattttcttt a
//
LOCUS       DAOQHY010000099        12434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2487_length_12434_cov_5.268438, whole genome shotgun sequence.
ACCESSION   DAOQHY010000099 DAOQHY010000000
VERSION     DAOQHY010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12434
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2487_length_12434_cov_5.268438"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PLL28_09120"
     CDS             complement(<1..348)
                     /locus_tag="PLL28_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNF69523.1"
                     /translation="MRGLLTLLFLLSGGFMLSSQAQVGRVETGIASYYHDKFVGRKTA
                     SGEIYSQDKMTAAHKSLPLGTWVKVTNLSNDSTVIVKINDRMPKSNGRSIDLTELAAS
                     KLNYLAKGLTKVRI"
     gene            complement(446..817)
                     /locus_tag="PLL28_09125"
     CDS             complement(446..817)
                     /locus_tag="PLL28_09125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69524.1"
                     /translation="MLKRILFLLLCIGVYTTIHAQSVSNDGVKITPTAEAKIGAGVTL
                     LLSPNPAPKSNPVITVTAVGMDIYSYKVVSATGQIVELENLSGKPDGTIIDLNGAVDI
                     GTYYVIFETNAGKISRKLVII"
     gene            complement(890..1384)
                     /locus_tag="PLL28_09130"
     CDS             complement(890..1384)
                     /locus_tag="PLL28_09130"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNF69525.1"
                     /translation="MIHKKVYITSMQSPLGLLVIKGTDEAIIEIMFADTPLPTDHKIP
                     DVMKQCIVQLEEYFEGTRQSFDIAIAPQGTDFQIQVWELLLRIGYAGTETYAELSKLL
                     GDEKKARAVGNATGKNPIAIVVPCHRLLGSDNSLTGYAGGLWRKQWLLDHEAEHGRGI
                     KKLF"
     gene            complement(1416..1886)
                     /locus_tag="PLL28_09135"
     CDS             complement(1416..1886)
                     /locus_tag="PLL28_09135"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HNF69526.1"
                     /translation="MYKFISVLLLTIVMNNLRAQAESTISTQFQWMIGTWEISIGEEK
                     IVEVWTSPHPDTLKGYSCLKYGDNTDTLELISITMKNGIIAYGAKVIGENGGEIVYFP
                     FVGYQDGWFVFQNLTHDYPTHIAYMRTNENTLDAYISGNGNSEPARFRYTKSDS"
     gene            1968..3467
                     /locus_tag="PLL28_09140"
     CDS             1968..3467
                     /locus_tag="PLL28_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNF69527.1"
                     /translation="MGLKGMLAKPFAAWVARKVRKDAANAIAIQQAVMLNLVRDAAHT
                     AFGKDHGFADIKTHDDFKRQVPVRDYEGLKAYIDRMVSGEKDVLWPGLPLYLAKTSGT
                     TSGVKYIPLTKESIPFHIEAARNALLMYIHETGKTDFVEGKMIFLQGSPALDKKNGIN
                     VGRLSGIVYHFVPQYLQRNRMPSYEINCIEDWEEKVDAIAGVTANERMSLISGIPPWV
                     LMYFERLQQISGKQYIKDVFPDFSLFVYGGVNYEPYRAKIESIIGKKIDAIETYPASE
                     GFIAYQDSQQAHGLLLNVNAGMFFEFIPASEVFNADARRLSLAEVELDVNYAIIINSN
                     AGLWGYSIGDMVKFVSLSPFRIIVSGRIKHFISAFGEHVIGEEVEYAVLTAAADNHVD
                     IVEFTVAPQVNPANGLPYHEWFIEFGKAPADLEAFALQVDNYLQQKNIYYKDLITGHI
                     LRTLVITTVATDGFRNYMKSQGKLGGQNKMPRLSNDRTIADELKQWTVC"
     gene            3527..4846
                     /gene="rseP"
                     /locus_tag="PLL28_09145"
     CDS             3527..4846
                     /gene="rseP"
                     /locus_tag="PLL28_09145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNF69528.1"
                     /translation="MQVLIQVAQLILALTILVFIHELGHFLAARAFGVKVEKFFIFFD
                     AWGKKIWSFKRGDTEYGIGWLPLGGYVKIVGMIDESLDKEQLKNEPQPWELRAKPNWQ
                     KFIVMIAGIVMNVILGVILYIIIHSRYSESYTTTAGVNNDGGIYAAEAGRKYGFETGD
                     KLVSINGKSYERIDDYLSARLYFGSTVIVDRNGKQVEVVVPDSAFRDKMRGKQIFDLR
                     ASIKIDGLSDSLTNARDAGMKKGDIMAMIDAVPVTCFSQMAEMLSAHKNDTAMISVDR
                     GGNMVALAIPVDSMGKIGIETNVKLYPYDSVAYSLGKSIHYGTRDAFEVFYFQAISLW
                     RIFTGDIPAKDSISSPIGISKMFSPTWDWGAFWMLTALLSMVLAFMNLLPIPALDGGH
                     ILFIGIESITGRKLSDKFMERAQVVGMVMLLMLMVFAVGNDILRMIN"
     gene            4850..5677
                     /locus_tag="PLL28_09150"
     CDS             4850..5677
                     /locus_tag="PLL28_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69529.1"
                     /translation="MEKALLYIALLMALPLCSQAQNSGQLLGTDSAVHRVMVIPFNQY
                     NYLSDADPELAKKNEVEAAEISRMFRYGLNYNVSARVISNHGTYNILTDTTVQSKRDL
                     EMIYGSVTYRFEKPMDITTPDTAADHSIHNPDLFGNGGAEPAVEEKKKLSFKKDDEPQ
                     KADKYLNAVVQKPEIFSTLQQRYGADIFIFINQFELVTNYQHCLDRTKNYFERKVVVH
                     YTIYDASGTQLKGDAMTVTFSSEQTNVDEIIATQFPLIADYLSTTVATVTNPPAGNK"
     gene            5674..6198
                     /gene="hscB"
                     /locus_tag="PLL28_09155"
     CDS             5674..6198
                     /gene="hscB"
                     /locus_tag="PLL28_09155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HNF69530.1"
                     /translation="MTYFELFNIPVSFLPDETYIRQQYFALSKKYHPDFAATLPIDEQ
                     VQTLEHATLVNKAYQVLSNFERRMQYILQEKGIIEPEGKFNLPPDFLMEMMDLNEEAM
                     QLKMQPDSGRVGQLQQRLQQQDTALLEAIKPLLEHYNVQSATSDEYASIKEFYYKRRY
                     ILRLKEQINNFARS"
     gene            6229..6313
                     /locus_tag="PLL28_09160"
     tRNA            6229..6313
                     /locus_tag="PLL28_09160"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6263..6265,aa:Leu,seq:caa)
     gene            6503..6811
                     /gene="rplU"
                     /locus_tag="PLL28_09165"
     CDS             6503..6811
                     /gene="rplU"
                     /locus_tag="PLL28_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNF69531.1"
                     /translation="MYAIVNIAGQQFKVEENQEIFVHRQTGNEGDKLEFSEVLLKARD
                     GKVEVGAPTVAKASVSATILSHMKGDKVIIFKKKRRKNYRVKNGHRQSFTKIKIDKIS
                     "
     gene            6833..7093
                     /gene="rpmA"
                     /locus_tag="PLL28_09170"
     CDS             6833..7093
                     /gene="rpmA"
                     /locus_tag="PLL28_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNF69532.1"
                     /translation="MAHKKGVGSSKNGRESHSKRLGVKVWGGQPVIAGNIIIRQRGTR
                     FHPGAGVGLGKDHTIFALVDGIVTFKKGREDRNIVSVLPAEA"
     gene            7244..7567
                     /locus_tag="PLL28_09175"
     CDS             7244..7567
                     /locus_tag="PLL28_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNF69533.1"
                     /translation="MRRDVFQAIADPTRRAIIGLIALQAMTPNALAEHFDMSRQAVSK
                     HLRILTECELVKPEQQGREIYYLLEMEKMKEVDQWLEQFRKIWVARFDELDGVLDRMK
                     KKNKG"
     gene            7600..8010
                     /locus_tag="PLL28_09180"
     CDS             7600..8010
                     /locus_tag="PLL28_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNF69534.1"
                     /translation="MATKVNIHATVAAPREKVWACYTEPEHITKWNFAADDWQCPSAS
                     NDMRVGGKYTARMEAKDGSFGFDFEAIYNAIDPGNSFTYTIPDGRVVDVVFSDNGSNT
                     DVAITFDAESVNPVELQQAGWQSILNNFKEYTEG"
     gene            complement(8029..9147)
                     /locus_tag="PLL28_09185"
     CDS             complement(8029..9147)
                     /locus_tag="PLL28_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF69535.1"
                     /translation="MRTLLILLRKEFQQIRRNPSILRIIFLMPAVQLLILPLAADYEV
                     KHVNLTIVNNDHSTYAQRLVQKFEVSDHFNITGYVDNYTEALQTIEQDKADIIITIPD
                     HFERDLIREDKSELHLAVNAVNGAKGNIGSAYAMTIINQFNQEVRSEWIVFPKTNPVP
                     IIDVTTINRFNDDMKYSLFMVPGILVILVTMVGSFLASLNIVHEKEVGTIEQLNVSPI
                     KKYQFILGKLIPFWLLGFVVLTIGLLIARLVYGIIPEGSLGLIYLFASVYLLALLGIG
                     LLISTLAETQQQAMLMSFFFMMVFILMGGLYTSIDGMPHWAQVVTRFNPVRYFIEVMR
                     MVIMKGSGLRDILPNLYAIGAMAIFFNTLAILSYRKRS"
     gene            complement(9144..10301)
                     /locus_tag="PLL28_09190"
     CDS             complement(9144..10301)
                     /locus_tag="PLL28_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF69536.1"
                     /translation="MPSLLNWRVVLKEPPTDMRQYGVFIKKEFKHIIRDKRSLLILFA
                     LPIAQILIFGFALTNEIKNTKIAILDLSQDEASHQLRDQLIASQYFELNGDVHDVADV
                     ESALLSDQIKLAVIIPENFQNDLSHGNEAQVQLIADATDPNTATTLSVYASAVIRDFQ
                     GDINGAYNLPYTITPEVRMLYNPQLKGAYLFVPGVMGMILLLISAMMTSIAIVREKEI
                     NTMEIILASPLKPIVLILSKAVPYITISLVNVTTILLISIFILGLPVAGNIFLLFAVC
                     FLFITTAIALGLMISNITDSQQTAMFISLMGLMLPTLILSGFMFPIENMPVPMQVLSN
                     IVPTKWFFIIVKDVMIKGLGFASIWREVLILTGMTLFFLFVSYKKFKTRLA"
     gene            complement(10252..11004)
                     /locus_tag="PLL28_09195"
     CDS             complement(10252..11004)
                     /locus_tag="PLL28_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF69537.1"
                     /translation="MHKEGQIVISASELTKTFGDFTAVDHISFEVKKGEIFGFLGANG
                     AGKTTAMKMLIGILSPTSGNASVAGYDIYTQTEHVKQNIGYMSQRFSLYEDIPVIENI
                     RFFGGIYGVGAKELKAKSAALLDKLELTSSAKTLVRDLPLGWKQKLAFSISILHDPQV
                     VFLDEPTGGVDPITRRKFWELIYEAADRGVTVFVTTHYMDEAEYCDRISIMVDGRIAA
                     MDKPAILKEQFQSPTIDAIFTQLARGAKRTAD"
     gene            complement(10997..11914)
                     /locus_tag="PLL28_09200"
     CDS             complement(10997..11914)
                     /locus_tag="PLL28_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF69538.1"
                     /translation="MESAIHIHNLHKTFNKGKLVALDNINFDVRKGELFGLIGPDGAG
                     KTSLFRILTTLWLADSGNATVAGLDVVKDFKEIRRRIGYMPGKFSLYQDLSVEENLNF
                     FATLFNTSIEENYDLVKDIYVQLEPFKSRKAGKLSGGMKQKLALCCALIHKPEVLFLD
                     EPTTGVDPVSRKEFWEMLKRLQSRITIIVSTPYMDEATLCDRIALMQGGKIMQLDTPQ
                     QIVDRFADELIAVRSEQMYHLIRDIRELPDIKGCYGFGDYLHVRFAHGAWTQERLTVA
                     LKDRHTLLEFCPITPGIEDCFMALMESHA"
     gene            complement(11920..>12434)
                     /locus_tag="PLL28_09205"
     CDS             complement(11920..>12434)
                     /locus_tag="PLL28_09205"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNF69539.1"
                     /translation="DDIVNQIAVLEKQLDAQRTSLMTTAGSIYAEVNPLTLQIAQIDD
                     QLVQSRVLNPIKGTVLTKYAETGEYTAPGKPVYKIADLTEMQLRAYVTGDQLPGIKIG
                     QQVKVLVDSADGQYSEHAGTVSWISDKAEFTPKSIMTKDERANLVYAIKITVPNTGNI
                     KIGMYGEVVF"
BASE COUNT         3641 a         2616 c         2669 g         3508 t
ORIGIN      
        1 aatacgcacc tttgttaatc ccttagccag ataattcaat ttcgaagcag caagctcagt
       61 aaggtcgatg gagcgcccgt tgctcttagg catgcggtcg ttaattttca cgattactgt
      121 ggagtcatta ctcaaattgg tcacttttac ccacgtacca agtggcagac ttttatgggc
      181 ggcggtcatt ttatcctgac tatagatctc tccgcttgca gtttttcgcc caacaaattt
      241 gtcgtggtag tagctggcaa tgcctgtttc tacgcggccc acctgagcct gcgacgaaag
      301 cataaagcct ccggaaagta gaaaaagtaa ggttaaaagc ccacgcatac aacggtttga
      361 ggtatccaaa ttacctcata cccctgttaa aggcaaaaat caggccgctt tgttatccac
      421 aatgggccaa atcgaaatat aagtatcaga taatcaccaa cttacgactg atctttcctg
      481 cgtttgtttc aaagatcacg tagtaagttc ctatatcaac ggccccgttg aggtcaataa
      541 tggtgccgtc aggtttgccg cttagattct ccagttcaac gatttgaccg gtagcggaaa
      601 caactttata agagtagata tccattccaa cggcagtcac agtaattacc ggattgcttt
      661 ttggtgccgg attcggacta agcaacagcg ttacgcctgc tcctatcttt gcttctgcag
      721 ttggggtaat cttaacacca tcattggaaa cagattgtgc atggatagtg gtatatacac
      781 ctatacagag gagcaaaaag aggatacgtt tgagcatggc tggtaacttt gacaataaca
      841 aaaataatcg aaatttttaa ttaggcaagc caacctgtca aatatgacat tagaataact
      901 ttttaatgcc acgcccgtgc tcagcttcat ggtccaataa ccactgcttg cgccacaatc
      961 cgcctgcata tccggtaaga ctattatcgg agccgagcag tcggtgacaa ggcaccacaa
     1021 tggcaattgg atttttaccc gtagcattgc ctactgccct tgctttcttt tcatcaccca
     1081 acaatttcga caattctgca taagtctctg ttcctgcata gccaatccgc aacagtaatt
     1141 cccaaacttg aatctgaaaa tccgttcctt gaggagcaat tgcaatatca aagctttgcc
     1201 gagtgccttc aaaatactcc tctaactgaa cgatacactg tttcatcaca tctggtatct
     1261 tgtgatcagt cggcaaagga gtatctgcaa acatgatctc tatgatggcc tcatcggttc
     1321 cttttattac cagcaatccc agtggcgact gcatggaggt gatatagacc ttcttatgta
     1381 tcaaggatat atttatttaa aataaaggta agcaatcaac tatcagattt tgtatatctg
     1441 aagcgtgccg gctcggaatt accattaccg gaaatatagg catcgagggt attctcattt
     1501 gtgcgcatgt aagcaatatg agtaggatag tcatgcgtta agttttgaaa gacaaaccaa
     1561 ccatcttgat atccgacaaa agggaaataa acaatctcgc cgccattttc cccgatcact
     1621 tttgcgccat aggcaattat gccatttttc atggtgatgg aaattaattc aagtgtgtca
     1681 gtattatcac catatttaag acaactgtat cctttcaaag tatcaggatg cggggatgtc
     1741 catacctcca caatcttttc ttctccgata gagatttccc aggtgcctat catccattga
     1801 aattgagtag agatggtgct ttccgcctga gcccttaggt tgttcatcac tatggtcagc
     1861 aataacaccg aaataaattt atacatggct taaagataca tgcttttaca gggatgtgaa
     1921 cgacaaagcc tattgggtaa ttgcacaaac atgcgcatct ttgtgccatg gggctgaaag
     1981 ggatgttagc gaaaccgttt gccgcctggg tagcgcggaa agttcgcaag gatgcagcaa
     2041 atgccatcgc catacaacag gcggtgatgc tgaatcttgt gcgtgatgcg gcacatacgg
     2101 catttggaaa ggatcatgga tttgccgata tcaaaacaca cgatgacttc aaacgccagg
     2161 ttcctgttcg cgactatgaa ggattgaaag cctatattga taggatggtt tcaggtgaaa
     2221 aagatgtact ctggcccgga ttaccactat atcttgccaa gacctcaggc actacatcag
     2281 gtgtaaaata tatccctctc accaaagaat ctattccatt tcacatcgaa gcggccagaa
     2341 atgcattgtt gatgtacatt catgaaaccg gaaaaacgga tttcgttgaa gggaaaatga
     2401 tctttttaca aggctcaccg gcgcttgaca aaaaaaatgg catcaatgta gggcgtttat
     2461 caggaatcgt ttatcatttt gttcctcaat atctgcaacg gaatcggatg ccttcgtatg
     2521 agatcaattg tattgaagat tgggaagaaa aggtagatgc tattgctgga gtaactgcaa
     2581 atgaacgcat gagtctgatc agcggcattc caccatgggt gctgatgtat ttcgaaagat
     2641 tgcaacagat ctccggaaag caatatatca aagatgtgtt tcccgatttt tcattgtttg
     2701 tatatggtgg agtgaattat gagccataca gagcaaaaat cgagtcaatt atcggaaaga
     2761 aaatagatgc catcgagact tatcctgcca gtgaaggatt tattgcctat caggattcac
     2821 agcaagcaca tggattgttg ctcaatgtaa atgcaggtat gtttttcgaa tttattcctg
     2881 cttcggaagt atttaatgcg gatgcgcgaa gactttcact tgcagaagta gaactcgacg
     2941 taaactacgc aatcatcatt aacagcaatg ccggactttg gggttatagc attggcgata
     3001 tggtgaaatt tgtgagttta tcgcctttcc gaattatcgt ttccggtaga ataaaacatt
     3061 ttatttctgc cttcggcgaa cacgtgatag gggaggaagt agaatatgcc gtattgacag
     3121 cagcggcaga taaccatgtg gatatagtgg agttcactgt agccccgcag gtaaacccgg
     3181 cgaacggatt accttatcac gaatggttta tagaattcgg taaggcacct gccgatttgg
     3241 aggcatttgc attgcaggtc gacaactatc tgcaacaaaa gaatatctat tacaaagacc
     3301 tgataacagg gcatatatta aggactctgg tgatcacaac ggtggcgact gatggtttca
     3361 ggaactacat gaaaagccag ggtaaactgg gaggacaaaa caagatgcct cgactcagca
     3421 acgaccgaac cattgccgat gaactaaaac aatggacagt gtgttaacaa agtttaatct
     3481 cattagtggt taaatcccac gtttattatc gaatttcgca ggcattatgc aagtattgat
     3541 ccaggtagca cagcttattt tagcactcac catactcgtt ttcatacatg aattagggca
     3601 ctttttagcc gccagagcct tcggtgtgaa ggtggagaaa ttcttcattt ttttcgatgc
     3661 ctggggaaag aagatatggt cattcaaacg cggtgatacc gaatatggta tcggttggtt
     3721 gcctttagga ggatatgtga aaattgtggg gatgatagat gaaagtctcg acaaagagca
     3781 attgaaaaat gagccgcaac cctgggaatt acgtgcgaaa ccaaattggc agaagtttat
     3841 tgtgatgatt gccggtattg tcatgaatgt gattcttggt gtcatcctgt atattattat
     3901 tcatagtcgc tattcagagt catataccac tactgccggt gtgaataacg atggtggtat
     3961 ttatgctgcc gaagcaggcc gcaaatatgg gtttgagaca ggcgataaat tggtatccat
     4021 caatggaaaa tcttacgaac gcattgatga ctatttatca gctcgtttgt atttcggatc
     4081 tactgtcatt gttgatcgca acggaaaaca agttgaagta gttgtgcctg atagtgcgtt
     4141 cagagataaa atgcgcggca agcaaatttt tgatttgcgg gcaagtatta aaatcgatgg
     4201 actctcggat tcacttacca atgcgcgtga tgcgggtatg aaaaaaggtg atatcatggc
     4261 gatgatcgat gctgttcccg ttacttgttt ttcgcagatg gctgaaatgt tatccgctca
     4321 caaaaatgat acagccatga tatccgtgga tcgcggaggc aatatggtgg cattggcaat
     4381 tcctgtcgac agcatgggaa aaatcggtat cgaaacaaat gtgaaattgt atccttacga
     4441 ttcagtggca tattcgcttg gaaaatcgat acattatggc acaagagacg catttgaagt
     4501 attttatttt caggccataa gcttgtggcg cattttcaca ggtgatattc ctgcaaaaga
     4561 ttctatcagc agtccgattg ggatttctaa aatgtttagt cctacatggg actggggtgc
     4621 cttctggatg ttgacggctt tattgagtat ggtattggca tttatgaatt tgttgccaat
     4681 tcctgcgctt gatggcgggc atattttatt tattggaatt gagagtatta caggaagaaa
     4741 gttatcggat aaattcatgg agcgcgcgca ggttgtggga atggtaatgc ttttaatgct
     4801 gatggtgttt gcagttggta atgatattct tcgcatgata aattaaccga tggaaaaagc
     4861 gctgctatat attgcgttgt tgatggcatt gccattgtgt tcgcaagcgc aaaattccgg
     4921 tcaattgtta ggcacagatt ccgcagtgca tagggtgatg gtaattccat ttaaccaata
     4981 caattatctc tccgatgcag accctgaact ggcgaaaaag aatgaagtgg aggcggccga
     5041 aatttcgcgc atgttccgtt atggattgaa ttataatgtc agcgctcggg tgatttcaaa
     5101 ccacggcacc tacaatattc ttaccgatac tacagtgcaa tccaaacgtg atcttgaaat
     5161 gatatatggg tcagttacct atcgttttga gaagccgatg gatattacca ctccggatac
     5221 cgccgcagat catagtattc acaatccgga tttgtttggc aacggtggtg ctgaacctgc
     5281 ggtagaggaa aagaagaagt tatcctttaa gaaagatgat gagccgcaaa aggccgataa
     5341 atatttaaat gcggtagtgc agaagcctga aattttcagt acgctgcagc aacggtatgg
     5401 tgccgatatt tttattttca tcaatcagtt tgaattagta actaattatc agcactgcct
     5461 tgatcgcaca aaaaattact ttgagcgaaa agttgttgtt cactatacca tttacgatgc
     5521 ttcaggcact caattaaaag gcgatgcgat gacagtgaca ttcagcagtg aacaaacaaa
     5581 tgtcgatgag atcattgcga cccaatttcc gttgattgca gattatttat cgaccacggt
     5641 agcaaccgta actaacccac cggcaggcaa caaatgacgt acttcgagtt atttaatatt
     5701 ccggtaagtt ttcttcccga tgaaacatat atcaggcagc aatatttcgc gctgagtaaa
     5761 aaatatcacc ctgatttcgc ggccaccttg cccattgatg aacaagtaca aacccttgag
     5821 catgccacgc ttgtaaacaa agcctatcag gtgttgagca attttgagcg aaggatgcaa
     5881 tatatacttc aagaaaaggg catcattgaa cctgagggta aattcaattt accacctgat
     5941 tttctgatgg aaatgatgga tttgaacgaa gaggccatgc aactgaaaat gcaacctgat
     6001 tccggacgag tagggcaatt gcaacaacgc cttcaacagc aggataccgc acttttggaa
     6061 gcaatcaaac cccttctcga acactacaac gtgcaatccg ccacctccga tgaatatgca
     6121 tcaatcaagg aattttacta taaaagacga tatatattgc gattgaagga gcaaatcaat
     6181 aactttgccc gctcctaaca ctaaaccgga cctcaatctg gtttttttgc cgaagtggtg
     6241 gaactggtag acacgccgga ctcaaaatcc ggtgccctca aaaagcgtgc gggttcgatt
     6301 cccgccttcg gtacttctcc tgcaattttc ctgctgtttt tcactttaat gcctccgcta
     6361 gccctaattg cctgtgttca taccctaatt tatggcgtaa ctaattgatt atcaatagct
     6421 aataaattcc ttaatattaa ttttctctat cctttgtcta aattatttgg attcattatc
     6481 tttgcacctt cattgaaaag tcatgtacgc gatagtaaac atagccggtc agcagttcaa
     6541 ggttgaagaa aaccaggaga tttttgtgca tcgccaaacg ggaaatgagg gcgataagct
     6601 ggaattcagc gaagttctgc tcaaggcccg tgatggtaaa gtggaagttg gagctcccac
     6661 agttgccaaa gcgagtgtat ctgctaccat cctttctcat atgaaaggcg ataaggtgat
     6721 catcttcaaa aagaaacgcc gcaaaaatta ccgagtgaaa aacggacatc gtcagtcttt
     6781 caccaagatc aaaatcgata aaatctccta attttttaaa tatttaagca tcatggcaca
     6841 taaaaaaggt gttggtagtt ccaagaacgg tcgcgaaagt catagcaaac gcctcggcgt
     6901 taaagtttgg ggaggtcaac ctgtaattgc aggaaacatt atcatccgtc agcgtggaac
     6961 acgtttccat ccgggagccg gtgtagggct tggtaaagac cacaccattt tcgccctcgt
     7021 tgatggtatc gtaaccttta aaaaaggtcg cgaagatcgc aatatcgtta gcgtattacc
     7081 tgccgaagca taagctaagc gcacaaccat attcaaaacc cttgtcggcc gacaagggtt
     7141 tttttgttgc ctgttacttc gaaaaatctc ccgcaaatat ttgcgaaatt gattggttgc
     7201 atgtaattta gcaaccaaat ggtttcctaa attgtccgtt actatgagaa gggatgtatt
     7261 tcaagctatt gctgatccaa ccagacgtgc cattattgga ctcattgcct tgcaagccat
     7321 gacaccaaat gcactggcag aacatttcga tatgtcgagg caagctgtat ccaaacacct
     7381 tcgtatactt acggaatgtg aactggtgaa gccggaacag cagggtaggg agatctatta
     7441 tttattggag atggaaaaaa tgaaagaggt agatcaatgg ttggagcaat ttaggaaaat
     7501 atgggtagca cgtttcgatg aactggacgg agtgctcgat cgaatgaaga aaaagaacaa
     7561 ggggtaatta atattcaaca aactcaaata aacaataaca tggcaactaa agtaaacatt
     7621 cacgcaactg ttgctgcacc gcgtgaaaaa gtgtgggcat gctacactga accggaacac
     7681 atcacaaagt ggaatttcgc tgccgacgat tggcaatgtc cttctgccag taacgatatg
     7741 cgtgttggcg gaaaatatac tgctcgcatg gaggcgaaag atggtagttt cggatttgac
     7801 ttcgaagcaa tatataatgc aattgatccg ggcaattcat ttacttatac cattcctgat
     7861 ggaagggtgg tggatgtagt attttccgac aatggaagca atacggatgt tgctatcacc
     7921 ttcgatgcag aatccgtaaa tcctgtggaa ttgcagcagg caggatggca gtcaatcctg
     7981 aataatttta aagaatatac agaagggtga ttgcaagatc accttttttt atgagcgttt
     8041 acgataggat aatattgcca gtgtattgaa aaatattgcc atcgcgccaa tagcataaag
     8101 atttggtaaa atatcacgca acccgcttcc cttcatgata accatacgca tcacttcgat
     8161 aaagtaacga acaggattaa atcgcgttac cacctgtgcc caatgcggca ttccatcaat
     8221 ggaagtatat aatcctccca tcagaataaa taccatcata aagaaaaacg acatcagcat
     8281 agcttgttgt tgtgtttccg caagggttga aatcaataat cctattccca acaacgctaa
     8341 taaatacacg gaagcgaata aataaatcaa ccccaaactg ccttccggaa ttatgccgta
     8401 cactaatcgc gcaatcagca aaccaatagt taataccaca aagcctaata gccagaaagg
     8461 aattaattta cctagtatga attgatattt tttgatcggc gatacattca attgctcgat
     8521 agtgcctact tctttttcat gcacaatgtt caatgaggca aggaaacttc ctaccatagt
     8581 caccagaatt accagaatac cgggaaccat gaacaatgag tatttcatat catcattaaa
     8641 tctattgatg gtagtaacat ctataattgg aacaggattt gtttttggaa atactatcca
     8701 ttcacttctg acctcctgat tgaattgatt gataattgtc atcgcatatg cagagccgat
     8761 atttcccttg gcaccattta cagcattcac cgcaagatgc aattctgatt tatcttctct
     8821 tatcaggtcg cgttcaaaat gatccggaat ggtaataata atatccgctt tatcttgttc
     8881 aattgtttgt aatgcctccg tataattatc tacatagccg gtaatattaa aatgatcact
     8941 cacctcaaat ttctgcacca atcgctgagc ataggtgctg tgatcattat ttacaatggt
     9001 gaggttgaca tgctttactt cgtaatcggc tgcaagtggc agtataagca attgaactgc
     9061 cggcatgagg aaaataatgc gcaggatgga tggatttctg cgtatctgct ggaattcttt
     9121 ccgcaataag ataagtagtg ttctcatgcc agccttgttt taaatttctt gtaactcaca
     9181 aataaaaaga acaaagtcat cccagtgaga atcaacactt cacgccagat actcgcaaat
     9241 cccaggcctt taatcatcac atcttttaca atgatgaaga accattttgt tggtacgata
     9301 ttactcaaaa cctgcatggg cacaggcatg ttttcaatcg ggaacatgaa gccactcaat
     9361 atcaatgtag gtaacatcaa tcccataagt gagataaaca ttgccgtttg ctgactgtcg
     9421 gttatattac tgatcatcaa tcccaatgca atagcggttg ttataaacag gaagcatact
     9481 gcaaaaagaa ggaaaatatt ccctgccacg ggcaatccga gaataaatat cgatatcagc
     9541 aaaatggtgg taacattcac aagtgagata gtgatatatg gaaccgcctt gctgagtatc
     9601 aatactatcg gttttaatgg tgatgcaagt ataatctcca ttgtattgat ttctttttcc
     9661 cgaacaatgg ctattgaagt catcatcgcg ctgatcaata acaatatcat acccatcaca
     9721 cccggcacga ataaataagc accttttaat tgcggattat atagcatgcg gacttccggc
     9781 gtaatcgtat atggcagatt ataggcacca ttgatatcac cctgaaaatc gcggataaca
     9841 gcacttgcat acacactcaa tgttgtagcc gtatttggat ctgtagcatc ggcgattaat
     9901 tgaacttgtg cctcgttgcc atgactaaga tcgttttgga aattttccgg tatgattact
     9961 gccaatttaa tttggtctga gagcagggca ctttctacgt cggctacatc atgcacatcg
    10021 ccgttcaatt caaaatattg actcgctatg agttgatcac gcaattgatg agaggcctca
    10081 tcctgagaaa gatcaagtat cgcgattttt gtatttttta tttcattggt cagcgcgaaa
    10141 ccgaaaatta aaatctgcgc aatgggcaag gcaaataata tcaacagcga tcgcttatcg
    10201 cggatgatat gtttaaattc ctttttgata aatactccgt attgtcgcat atcagtcggc
    10261 ggttctttta gcaccacgcg ccaattgagt aaagatggca tcaatggtag gtgactgaaa
    10321 ttgttctttc aaaatagcag gtttatccat tgcagcaata cgaccgtcga ccataatgga
    10381 tatacgatca caatattctg cttcgtccat ataatgtgtg gtaacgaaca cagtgactcc
    10441 tcgatctgct gcttcataaa tgagctccca gaattttctt cgagtaatag gatcgacacc
    10501 acccgttggt tcatcaagaa ataccacctg agggtcatgt aatattgaaa tcgagaatgc
    10561 cagtttttgc ttccaaccca aaggcaagtc gcgaacaagc gttttggcac tggatgtcaa
    10621 ttccagtttg tcgagaagcg ctgcactctt cgcttttaat tccttcgccc caacgccata
    10681 aatgccacca aaaaagcgaa tgttctcaat cacaggaata tcttcataca aagaaaagcg
    10741 ctgactcatg taaccaatat tttgttttac atgctctgtc tgcgtataaa tatcgtaacc
    10801 tgcaacagag gcattcccgg aggtaggaga gaggataccg atgagcattt tcatggcagt
    10861 agtttttccc gcgccattgg ctccaagaaa accaaatatc tcgcccttct ttacttcaaa
    10921 actgatatga tcaaccgcgg taaaatcacc aaaggtttta gtgagttcac ttgcactgat
    10981 tactatttgg ccttccttat gcatgcgatt ccattaaagc catgaaacaa tcttcgatgc
    11041 ccggggtgat ggggcagaat tccaataagg tatgtctatc cttcagtgca acagttaagc
    11101 gctcttgagt ccaggcaccg tgtgcaaagc gcacatgcag gtaatcaccg aaaccatagc
    11161 aacctttaat gtcgggcaat tctcggatat cacgtatcag gtgatacatc tgttcgcttc
    11221 ttacggcaat cagttcatct gcaaaacggt caactatctg ttgcggtgta tccaattgca
    11281 tgatctttcc tccctgcata agcgcaatcc tatcgcaaag cgtagcctca tccatatagg
    11341 gtgtactaac gattatggtg atgcgcgact gaaggcgttt caacatctcc cagaactctt
    11401 tccgtgaaac cggatctacg cctgtggttg gctcatccag aaacaatacc tcgggtttat
    11461 gaatcagcgc gcagcataag gcaagttttt gcttcatccc accactgagt ttacctgctt
    11521 tacggctttt aaatggctcg agttgcacgt aaatatcctt taccaaatcg tagttttcct
    11581 cgatgctggt gttgaacagg gtagcgaaga agttgaggtt ttcctctaca ctaaggtcct
    11641 gatacaaact gaacttgccc ggcatatacc caattcttcg gcgtatctcc ttgaaatctt
    11701 ttactacatc taatccggcc acagttgcat taccgctgtc ggcaagccat agggtagtga
    11761 ggatgcggaa aagggaggtc ttgccggcac catcgggacc tatcaaccca aaaagctcac
    11821 ctttgcgcac gtcgaagttg atattgtcga gtgcaacaag cttcccttta ttaaaggttt
    11881 tatgcaggtt atgtatgtgg atggcgcttt ccatgtgtat cagaatacta cttcgccata
    11941 cataccgatc ttgatatttc cggtattcgg tacggtgatc ttgatggcgt aaacaagatt
    12001 cgcacgttcg tctttcgtca taatgctttt aggggtgaat tcagccttgt cactgatcca
    12061 gctaacggtt ccggcatgtt cggaatattg accgtcggca ctgtctacca acaccttcac
    12121 ctgctgaccg atcttgatgc cgggcagttg gtcgccggta acataagcgc gcaattgcat
    12181 ttcagtgagg tcggcgatct tgtaaacagg ttttcccggg gcagtgtatt caccggtttc
    12241 agcatatttt gtcaatactg ttcctttaat cggattcagc acccttgatt gcaccaattg
    12301 gtcatcaatc tgggcgatct gcagggtgag agggtttact tcagcataga tactgccggc
    12361 ggtggtcatc aggcttgtac gctgcgcatc gagttgcttc tccagcaccg cgatctgatt
    12421 cacgatgtca tcga
//
LOCUS       DAOQHY010000100        12348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2506_length_12348_cov_6.235825, whole genome shotgun sequence.
ACCESSION   DAOQHY010000100 DAOQHY010000000
VERSION     DAOQHY010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12348
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2506_length_12348_cov_6.235825"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLL28_09210"
     CDS             <1..234
                     /locus_tag="PLL28_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNF69540.1"
                     /translation="MKEMSHLNGMDFAASMPDQFNLVINTNHPVVNKMLQLDETHRLN
                     TARQLHDLALLSQGMLKGTALTEFVKRSVEQMQ"
     gene            complement(480..614)
                     /locus_tag="PLL28_09215"
     CDS             complement(480..614)
                     /locus_tag="PLL28_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69541.1"
                     /translation="MKHLTQGKVSLKGGSESITYRNRLSGDDQPVCFDGCNFFGRYDK
                     "
     gene            687..2300
                     /locus_tag="PLL28_09220"
     CDS             687..2300
                     /locus_tag="PLL28_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69542.1"
                     /translation="MLHKLHIRRLFHITLLLVVIAGISSCGTTQFRKDYYNVNTLLHD
                     STQFATGTPWLKAHMKNGNIVIFYNTWEVDSAQTYISGFGAQYDFNRQLITQDHLSIP
                     IDDIALFETNNPVQDNKGRISAITIMTGLDILFGVVCITVPKACWGSCPTFYIDSTAT
                     THYADAEGFSNAILPSMQYGDIDALPGNVIPGSTFRITLRNEALETHCIDKVTLYAVS
                     TPSNKNAFQSVDNKFYLGDKNFICSKANDENGEITALLEKADTQERFSTTDAHNLKSK
                     EAIYLNFPSAQANDSIGLLLRFRQTLLPTYCYYNAVSYMGDFVGGINTLLETDEKIRN
                     KFGHDGFLGALGGIDVYVWNESENDWKYEGAFNETGPIAINTQILPLSAFDSKDSIKI
                     KLVLNKGLWRIDQAALVTDLHRTQAKAISPQVINAAGGHEDAFSDYLSDSTNYLITYP
                     GEHFYLDFPMPEQSDHYTAFLYSEGYYLEWMRGDWLADKNILKLRYMLNFPNLYLKTE
                     ASVFKEHEAAMEQIFWNSRISQPIFSSNE"
     gene            2293..2796
                     /locus_tag="PLL28_09225"
     CDS             2293..2796
                     /locus_tag="PLL28_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69543.1"
                     /translation="MNNKCTIIIIAVMLIASGCASYPKGINPVGKNKTDPYGATMKVS
                     PKSTKTIIGELIAVNGDSLYLLGANNQIMRYHIKDIRTYVVYYGTPEKTLGWTYALLP
                     AISISHGFWMILSIPINLIFAGGITNDVKQDMIYTNRELPIDKLSIMARFPQGLPPSV
                     SLNEITR"
     gene            complement(2819..3469)
                     /locus_tag="PLL28_09230"
     CDS             complement(2819..3469)
                     /locus_tag="PLL28_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNF69544.1"
                     /translation="MASIISFTFNAFYENTYVIYDRTGACVIVDPGCYDRAEEDDLVD
                     AISKKGLKPALLLNTHCHVDHVLGNKFVSDTYQIPLVMHRGELPILQNVVNYAPSMGL
                     RYRPSPDPERFVVDGDTITFGETTLDVLYTPGHSPASVCFYCKADNFIVSGDVLFHDS
                     IGRTDLPGGSMPTLMHSIRTKLMNLPDETKVYAGHMQSTTIGRERLHNPFLTGTYA"
     gene            3542..7000
                     /locus_tag="PLL28_09235"
     CDS             3542..7000
                     /locus_tag="PLL28_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69545.1"
                     /translation="MRRVPGMKIISTNRLRWFWLLAVLFSTNIAFGQGTQVQFGQNRV
                     QYKDFDWQFYEGEHFQVYFSAGGLNLGKFVAQIAEADLIQIEDLLDYKLNSKPEIIVY
                     NTISDYNQSNFAIGAEEQYNIGGQTKIIGNKVFIYFDGNHQHLRKQVREGVGKVLINS
                     MIFGGNLQEIVQNAVLLNLPEWFTEGLVSYIGEEWNTELDNQLRDGILSGRYQKFNKL
                     TGEDARFAGHALWYYIAESYGESTIPNLLYLIRINRSIENGFLFVLGTGVNGTIDAWY
                     NYFLTKYQKESAGKTLPALENAIPRKERKEVQFHQLALSPDGEHIAYAMDDHGKFKVF
                     MQEMDTKDRQKIKSGGLKTHTLATDLSNPLIAWSPDGKKLAIVYEKRYKNYITTYDTE
                     KKEKETKELTRFQKILHISFAKSSSKLVMSVVNNGQSDLYMYTLTNDRVEQLTNDYYD
                     DLDPVYVQLNNGYEGVIFASNRDLDTIFTTRAIDSILPVGNMDLYFYDYNRKSKDLVK
                     ITHTRRISESAPLQFDGKHFAYLSDQNGIINRFGGYLDSLYDHTDTLVYYPDSTVTNP
                     PYAYKDFTQFPKFDSVLLADIYRDTAYVFPVSNYASNILEHSINWKTGAMVETFKSGK
                     STVFLKAQAPSDIGALQQNALDNTTFRQFIDQKKKGDDVVMTINEKDTSSLITHQTII
                     TKTDTIHKEIDEGMYFQTDFPEPDPETKLLSEPVTLGNSANPEMQNINTGKKFKSSRI
                     TPYRLKFSSDYVTSQLDNSLFINKYESFNTTGGVYSTPNLGALLTVSISDLFEDYRFV
                     GGMRFPTSFSGGEYFLTYEDLKHRLDKKFTWYYQTDNQQYTFQPTWFPLVDAGLSTNI
                     VEAAFKWPIDVVRSVRGTVSYRAENITFLANDTFSLNLPDYSEDWLSVKFEYVHDNTW
                     PVMTNIMNGMRYKVWLEMHKQFNVDPNADVAVDFSEGYLGVLGFDVRHYQKIHRQIIW
                     ANRIAGGTSFGKQKLIYYLGGVDSWIAPNFNNDIEVNTDMGYAFQTLATNLRGYDQNI
                     RNGNSYAVLNSELRFPVFSYFFNKPVRSEFLRNLQVVGFYDIGSAWEGFSPFEEDNPY
                     NDATITQQPITVVVNYFRDPLVMGTGLGMRTTLFGYFIRVDRAQPIEAGGLGDPKWYF
                     SLSLDF"
     gene            7188..8384
                     /locus_tag="PLL28_09240"
     CDS             7188..8384
                     /locus_tag="PLL28_09240"
                     /inference="COORDINATES: protein motif:HMM:NF018479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HNF69546.1"
                     /translation="MSTIHNKIWWRNTNERNVGITDRKISWLELFYDLVYVIAISRAT
                     HHLSAHLTFEGFLDYFYMVALIYWGWLNGSWHHDILGANDVRTIFLTIWQMVILSALI
                     ATLGTGEEMNLFNVTIAILLMQLYISYLWWSEGIYDKTNKDVRLIYVGFYMLAFALIV
                     STLFIDPAYLRVVFYTALMINYIPPYFVTRRASHELRYEQRLSPSMTERLGLFTIILF
                     GEVILGVINGATAFEKLTAYEWSVFVAAIFMVFSIWYIFFKLIGDKHVKKGYVNSNLY
                     AFLFLPIAIALGFTGVSFENVLAAGHAHHTNVNTELFLGLSIALFLISIVLLTLLVES
                     KQVYVNAMPKMRRLLILVIAGIVVTSIISNSFSLGEFLMILLILLLILPVMILVLWNN
                     LELKKQ"
     gene            8485..9210
                     /locus_tag="PLL28_09245"
     CDS             8485..9210
                     /locus_tag="PLL28_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HNF69547.1"
                     /translation="MPTTQNKIEELKETGFTTVESLYSDTEIEQIIMAISHADSSKET
                     FRKSNDLFAIRQFLKEIPEVANYIFNDKLKKIISEYFGTNYFVVKSIYFDKPETSNWY
                     VAYQQDLTISVNQKIEIENFGPWTIKQNQFAVQPPIEFLENMYTIRIHLDDTDENNGA
                     LRVIPKSHVKKIYRPETIDWTTENESICKVAKGGIMIMKPLILHSSGRTTNNNKRRVI
                     HIEFSNTELPDGLHWAERMNVLN"
     gene            9542..10456
                     /locus_tag="PLL28_09250"
     CDS             9542..10456
                     /locus_tag="PLL28_09250"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNF69548.1"
                     /translation="MKPNVKAHIYVLAANIIYGINYSVGKVALKEMSPLAIVAFRVCC
                     AFLVFFAAHQIFIKENVDRADHKKFILASLFGVAVNQMLFFTGLSLTSEMHASLIMIT
                     TPLLVLIMAWVILRDPITITKSIGILSGAAGVALLIISGTVADNSTASAFGDLCIFIN
                     ATSYAIFLVIAKPLMKKYAPLTLAKWIFFYGMFMVIPFGFNDLQQLSFGEISTQAWLA
                     LSVVVFGATVLAYLFNILGLRYGSPALVSIYIYTQPIIATMVALMLGTDSLTLPKVIS
                     AILVFAGVALVSRSSIAIPAAEPEAKLK"
     gene            10482..11354
                     /locus_tag="PLL28_09255"
     CDS             10482..11354
                     /locus_tag="PLL28_09255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNF69549.1"
                     /translation="MLISMTGYGRTTFALNGKNIVLEVKTLNSKSFDLNLRIAPLFKE
                     YEMEIRNLLSKYIIRGKTDCLLTYEQMDEKAGEININAIKEYYRQIKSISDNMGLEQA
                     NIMDLVFRLPNITTSSNEPLTDEQWEMVTNHIDNTCGQLTKFRTVEGINLEQDLILHV
                     NLIHSALIEVEALDPKRMAQQREKMHNDLRSYLASENIDMNRFEQEVIFYLEKMDITE
                     EVIRLRSHCEYFMQTIEDKAQEKGKKLGFIAQEMNREINTIGSKAYHADMQRKVVEMK
                     DELEKIKEQLNNVL"
     gene            11354..11923
                     /gene="gmk"
                     /locus_tag="PLL28_09260"
     CDS             11354..11923
                     /gene="gmk"
                     /locus_tag="PLL28_09260"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNF69550.1"
                     /translation="MSGKLIIVTAPSGAGKTTIVRHLLAVEPLLAFSVSATTRQKRDY
                     EKDGVDYHFLSADQFRKQIEENAFVEWEEVYPGKFYGTLRSEVERMWAAGKHIIFDVD
                     VKGALSLKSKFPEKTLSLFISPPSVEILAQRLIDRGTETPSTLQTRLERVRYELTFSP
                     KFDHVIVNDVLETAKTEAQRLVHNFLNMA"
     gene            11972..>12348
                     /gene="apaG"
                     /locus_tag="PLL28_09265"
     CDS             11972..>12348
                     /gene="apaG"
                     /locus_tag="PLL28_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009187262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HNF69551.1"
                     /translation="MVTSITQGVKVSVETFYQQEFANKGEYVFAYRITIENKSEHTVQ
                     LLRRHWYIYDGIGTWREVEGEGVVGETPVIAPGHIHQYVSGCHLLAPAGKMHGSYLMM
                     RTLDGMYIDVTIPEFHMVAPFVL"
BASE COUNT         3817 a         2570 c         2441 g         3520 t
ORIGIN      
        1 atgaaagaga tgagtcacct caacggcatg gatttcgcag catcaatgcc tgatcaattc
       61 aatttggtaa ttaataccaa tcatcccgta gtaaataaaa tgctgcaact tgatgaaact
      121 catcgtttaa atacggcccg tcagcttcac gaccttgcat tattatcgca aggtatgctc
      181 aaaggcactg cgctcaccga atttgtaaag cgcagtgtgg agcagatgca gtaatgcact
      241 tataactttt ttccctgcac ttgttgcagc gattccaaac gcaccagttt gtaagccgga
      301 gagcaagcaa acaggtgcgt taatatttgt gcatgccccg ctccaataat caccagtatt
      361 ctatcgtcgg ggcttgtggt aatttgctga atatttctat aaatacgcaa atttcttgct
      421 taaaaatcca tagatgatgc atctgtgctt tcgcaattgc tttattaagg atacatcaat
      481 catttatcat agcgaccgaa aaaattacag ccatcgaagc aaacaggttg atcatcgccg
      541 gaaagcctat tccgataggt gatactttca gaaccgccct tcaggctcac cttcccttgc
      601 gttaggtgtt tcacgggtca tagatttcag cccatcagtg ataaaaatca tttgcatgct
      661 atggtaatta ttatcaactt tataatatgc tccataaact tcatatacga agattatttc
      721 atataactct gttactggta gttatagcag gcattagctc ttgcggcacc acacaattca
      781 ggaaggatta ctacaatgta aataccctat tacacgattc tacgcagttt gccacaggaa
      841 cgccctggct aaaagcacat atgaaaaatg gcaacattgt cattttttat aatacctggg
      901 aggttgacag tgctcaaacc tatatttccg gtttcggagc acaatatgat tttaacaggc
      961 aactgattac acaggatcat ctgagtattc ctattgatga cattgcatta tttgaaacca
     1021 acaatcccgt tcaggataat aagggtagaa tctcggctat caccatcatg accggtttgg
     1081 atattttgtt tggagtggta tgcattactg ttcccaaagc atgctgggga tcttgtccta
     1141 cattttatat cgactccacc gcaactacac attatgccga tgcagaaggt ttttcgaatg
     1201 ccattctacc atcaatgcaa tatggcgata tcgatgccct acccggcaat gttataccgg
     1261 gaagcacttt caggataacc ctgcgcaatg aagctttgga aacacattgc attgataaag
     1321 tgacattata tgcagtttct accccatcaa ataaaaatgc atttcaaagt gtcgacaata
     1381 aattttattt aggtgataaa aattttatct gctcaaaagc aaatgatgaa aatggagaga
     1441 tcactgccct gcttgaaaaa gcagatactc aggaacgttt ctcaactaca gatgcgcata
     1501 acttaaaaag caaagaagca atttacctca acttcccttc tgcacaagca aacgactcaa
     1561 taggtttatt gcttcgtttc agacaaacct tgctgccaac atattgttat tataacgccg
     1621 tgagctacat gggtgatttt gttggtggca tcaatacatt attggaaact gatgaaaaaa
     1681 tcagaaataa attcggtcac gacggtttcc ttggtgcttt aggaggcatt gatgtatatg
     1741 tgtggaatga atccgaaaat gattggaaat atgaaggcgc attcaatgaa acaggaccca
     1801 ttgccattaa cacgcaaata ttgccattat cagcttttga tagcaaggat tccataaaaa
     1861 taaaactggt actaaataaa ggattgtggc gtatagatca ggccgcattg gtgaccgatt
     1921 tacatcgtac acaagcaaag gcaatatcac cacaagtaat aaatgccgcc ggcggacatg
     1981 aagacgcctt ctccgattat ctttccgaca gcaccaatta tttgattact tatcctggtg
     2041 aacattttta tttagatttt ccaatgccgg aacaatcaga tcattatact gcctttttat
     2101 attcagaagg atactatctg gaatggatgc gcggcgattg gcttgcagat aaaaacatat
     2161 taaaacttcg gtacatgctc aatttcccaa atctatatct caaaaccgaa gcctctgtat
     2221 ttaaggaaca cgaagccgca atggagcaga ttttttggaa ttcaagaatt tcacaaccta
     2281 tatttagcag caatgaataa caaatgcaca ataatcatta ttgcagtaat gctcattgca
     2341 agcggctgcg cctcctatcc gaaaggcatc aatcctgtcg gaaaaaataa aactgatccc
     2401 tacggtgcaa ccatgaaggt gagccctaag tccactaaaa ccataattgg tgaacttatt
     2461 gcagttaatg gagatagttt atacctgctt ggtgcaaaca atcaaattat gcgttaccat
     2521 attaaggaca tccgaactta cgtggtatat tatggcaccc ctgaaaaaac cttaggttgg
     2581 acatatgctt tgcttcctgc aatttccatt tcacatggat tttggatgat actttctata
     2641 ccaatcaacc tgattttcgc cggaggaatt acgaatgatg ttaaacaaga catgatatac
     2701 actaatcgtg aattgcctat cgacaaatta agcatcatgg caagatttcc gcaaggcttg
     2761 ccgccttcag tctcactaaa tgaaataacc agataaaacc tggtcttttt cttttacttc
     2821 acgcataggt tccagtaaga aaaggattgt gtaatctttc tctgccgatg gttgtcgatt
     2881 gcatatgacc ggcatatact tttgtctcat cgggcaggtt cattaacttg gttctgatgc
     2941 tatgcattaa tgttggcata cttcctcctg gaagatctgt gcgacctata gagtcatgga
     3001 ataaaacatc accacttacg ataaaattat cggctttaca gtaaaaacat acactggcag
     3061 gagaatgccc cggggtatac aacacatcaa gggtggtttc gccaaaggtg atggtatcgc
     3121 cgtctaccac aaatctttca ggatcgggag aaggtcgata gcgcaagccc atggaagggg
     3181 cataattcac cacattttgc aatatcggaa gctcgcctct gtgcattacc aaggggattt
     3241 ggtaggtatc agaaacgaat ttattgccca aaacatgatc tacatggcag tgcgtattca
     3301 gcagcagcgc gggtttgagg cctttttttg aaatggcgtc taccagatca tcctcctcgg
     3361 cacggtcgta acaaccgggg tccacgatca cacaggcacc cgttctatca tagattacgt
     3421 atgtattttc gtagaaggca ttgaaggtga aagagataat agaggccatg tttctatcgt
     3481 taaattgtaa aattttaatg aagcgggcga actttacggc aaaagtatgt attttgcagt
     3541 ggtgagaagg gttccgggta tgaagatcat ttccacaaat cgactcagat ggttttggct
     3601 gctggcagtg ttgttcagta ccaatatcgc ttttggccaa ggcacccagg tccaattcgg
     3661 gcaaaatcgc gttcaataca aggatttcga ctggcaattt tatgagggcg aacactttca
     3721 ggtctatttc agtgcagggg ggctcaacct aggaaaattc gttgcacaaa tagcagaagc
     3781 cgatctgatt caaatagaag acctgctgga ttacaaactc aacagtaagc ccgaaattat
     3841 agtttacaat actatttcag attacaacca aagtaatttc gccattggag ctgaagagca
     3901 gtataatatt ggagggcaga ccaaaataat tggcaataaa gtatttattt atttcgatgg
     3961 caatcatcag catctgagaa aacaggtgcg cgaaggtgtg gggaaagtgc taatcaatag
     4021 catgatattt ggcggcaact tgcaggaaat cgttcaaaac gccgtactgc tgaatttacc
     4081 tgaatggttt accgaaggcc ttgtgtctta tattggcgaa gagtggaata cggaacttga
     4141 taatcagctg cgcgatggca ttttatccgg cagatatcaa aaattcaata agctgaccgg
     4201 tgaagatgcc cgattcgcag ggcatgcttt gtggtactat attgccgaaa gttatggcga
     4261 atcaactatt ccgaatttgt tgtatctcat tcgcattaac agaagtattg agaacggatt
     4321 cctatttgta ctcggcacag gagtaaacgg cactattgat gcatggtaca attatttcct
     4381 caccaaatac caaaaagaat ctgccgggaa aacattacct gcacttgaaa atgcgatacc
     4441 cagaaaagaa cgcaaggaag tgcaatttca tcaactggct ttaagccccg acggcgagca
     4501 tattgcctac gccatggatg atcatggtaa gtttaaagta ttcatgcagg aaatggatac
     4561 caaggaccga cagaaaatta aatccggtgg attaaagaca catacgcttg ctactgatct
     4621 cagcaatcca ctgattgcct ggagccccga tggtaaaaaa ctcgctatcg tttatgaaaa
     4681 gagatataag aattatatca ccacttacga taccgaaaaa aaggaaaaag aaacgaagga
     4741 actaacacgt ttccaaaaaa tattgcatat cagctttgcg aaatcatcct caaaattggt
     4801 gatgagtgta gtcaataacg ggcaaagcga tctctacatg tacaccttga ccaacgacag
     4861 ggttgaacag cttacaaatg attattatga tgatctggat cctgtgtatg tgcaattaaa
     4921 taatggctat gaaggtgtca ttttcgcgtc aaacagagac ctggatacta ttttcaccac
     4981 aagagccatc gattcgattt tacctgtggg caatatggat ttgtatttct atgattacaa
     5041 tcgcaaatcg aaagatcttg tcaaaatcac ccatacacgt cgcatcagcg aatcagcacc
     5101 tttgcaattc gatggcaaac attttgcata tctgagcgat caaaatggta tcataaaccg
     5161 gtttggaggg tatctggata gtttatatga ccataccgat acattagttt attatcccga
     5221 cagcacagtt accaatccac cttatgcata caaggatttc actcaatttc cgaaattcga
     5281 ctctgtttta ttggcagata tttatcgcga tacggcatat gtatttcctg taagcaatta
     5341 tgcttccaat attcttgaac atagcatcaa ttggaaaacc ggtgctatgg tggaaacatt
     5401 taaatcagga aaatcaactg tatttttaaa agcacaagca ccgtctgata ttggagcatt
     5461 gcaacaaaac gctctcgaca atacaacttt ccgtcaattt atcgatcaaa agaaaaaggg
     5521 cgacgatgtg gtgatgacca ttaatgaaaa ggatacaagt tcattgatca cacatcagac
     5581 catcatcaca aagaccgata ccatacacaa ggaaattgat gaaggcatgt atttccagac
     5641 agacttcccc gaacctgatc ctgaaacgaa attgctttct gaaccggtta cacttggcaa
     5701 ttcggctaac cctgaaatgc aaaatatcaa tacaggaaaa aaattcaaat catcccgcat
     5761 cacaccttat cgcttgaaat tttcaagtga ttatgtgaca agtcagttgg ataatagttt
     5821 atttattaac aaatacgaaa gcttcaatac gacaggcgga gtatattcga caccaaacct
     5881 cggagcattg ctcacggtca gcatcagcga tctgtttgaa gattaccgtt ttgtaggcgg
     5941 catgcgcttt ccaacatcat tttcgggtgg tgaatatttt cttacctatg aagatttaaa
     6001 acatcgtctg gataaaaaat tcacgtggta ttatcagaca gataatcagc aatatacttt
     6061 ccaacctaca tggttccctc tggttgatgc cggtttatca accaatatcg ttgaagctgc
     6121 attcaagtgg ccgatagatg tggtgagaag tgtacgcggt actgtttctt atcgcgcaga
     6181 aaatattacc tttttagcca atgacacctt cagtctcaat ttaccggatt attcagaaga
     6241 ttggttgtcg gtaaaatttg aatatgtgca tgataatacc tggccggtaa tgaccaatat
     6301 catgaatggc atgcgatata aagtatggct ggaaatgcac aaacaattta atgtagatcc
     6361 gaatgccgat gtggctgttg atttcagtga aggttatctt ggtgtgttgg gatttgatgt
     6421 gcgtcattat caaaaaattc atcgccagat catttgggcc aatcgcattg caggaggcac
     6481 ttccttcgga aaacaaaaat tgatctatta cctcggtggt gtagatagct ggatagctcc
     6541 gaatttcaat aacgatattg aagtaaatac cgacatggga tatgcttttc agaccttggc
     6601 gacaaatttg cgcggttacg atcagaatat tcgcaatgga aacagctatg cggtattaaa
     6661 ttcggaattg cgtttccctg ttttctctta cttctttaat aagcctgtgc gttctgaatt
     6721 tttgcgcaat ttacaggtgg ttggatttta tgatatcggt agtgcatggg aaggattttc
     6781 tccttttgaa gaggataatc cttataacga tgccaccatt acgcagcagc cgataacagt
     6841 tgttgtaaat tatttccgcg acccattggt tatgggaaca ggcttaggaa tgcgcactac
     6901 ccttttcgga tacttcattc gcgtagatcg cgctcaaccc atagaagccg gcggcctggg
     6961 tgatccaaaa tggtatttct ctctcagttt ggatttttga ttgtaagtgg gtttatggtt
     7021 tgtgctattt ttcgcgctaa gacgctaaga tgcaaagccg caaagcttta actcacattc
     7081 accactcacc attcaccatt cacccactca ccactcacca ttcacccctc acccctttga
     7141 attccccacc acatcccata cttttacaat tgctcaaata gcattcaatg tcaactatac
     7201 acaacaaaat atggtggagg aataccaatg aacgaaatgt tggtattacg gatagaaaaa
     7261 tcagttggct ggaattgttt tatgatttag tgtatgtgat tgcaatatcc agggcaactc
     7321 accatttatc ggcacatctg acatttgaag gattcttgga ttacttttat atggttgctc
     7381 tgatttattg gggttggctc aatggcagct ggcatcatga tattcttgga gcaaatgatg
     7441 tacggacaat tttcctgaca atatggcaaa tggtcatatt atcagcacta atagctaccc
     7501 ttggaacagg cgaggagatg aatttattta atgtcaccat tgctatatta ttgatgcaat
     7561 tatatatttc atatttatgg tggagtgaag gcatttatga taaaacaaat aaagatgtca
     7621 gattaattta cgtaggcttc tacatgctgg cctttgcgct gattgtcagc acactgttta
     7681 ttgatccggc atacctacgc gttgttttct atacggcctt aatgatcaat tacattccac
     7741 catacttcgt aactcgaagg gcaagtcacg agcttcgata tgaacaacgc ttgtctccaa
     7801 gtatgacaga acgcctggga ttgtttacga taattttgtt tggtgaagtg attctcggtg
     7861 ttataaatgg agcgacagca tttgaaaaat tgacagcata cgaatggtcg gtattcgtgg
     7921 ctgcaatatt tatggtgttt tcgatttggt atatattctt caaattgatt ggcgacaagc
     7981 acgtaaaaaa gggttacgtt aacagcaatt tgtatgcatt tctatttctg ccgattgcaa
     8041 tcgcattagg attcacaggt gtatcgtttg aaaatgtgtt ggccgcaggt cacgcacatc
     8101 atacaaatgt aaatacggaa ttatttttag gattatcaat tgcgctgttt ctcatttcta
     8161 ttgtgttgct aacactgctg gtagaatcta agcaggtata tgtaaacgct atgcccaaaa
     8221 tgaggcgttt gcttattttg gtcatcgccg gaatagtagt gacatcaata atatcaaaca
     8281 gcttttcatt gggcgaattc ttaatgattt tactgattct actcctcatt ttaccggtta
     8341 tgatattggt gttgtggaat aatctggagt taaaaaaaca ataacaccaa tcaacttcct
     8401 aatatgtatc ccgtctgaaa cgttgggcac tttaatttca cttctctatc tgcacaacac
     8461 cactactcaa tccataacca caaaatgccc acaacccaaa acaaaattga agagctcaaa
     8521 gaaacaggat ttacaactgt tgaaagcctc tattccgata ctgaaatcga gcaaataatt
     8581 atggctattt cacatgccga cagcagtaaa gaaactttta gaaaatcaaa cgaccttttt
     8641 gcaatcagac aatttttaaa ggaaattccg gaggtagcga attatatatt caatgataaa
     8701 ttgaaaaaaa tcatctccga atattttggc acaaattatt tcgttgtcaa aagcatttat
     8761 ttcgacaagc ctgaaacttc gaattggtat gtggcctatc aacaggatct aactatttca
     8821 gttaatcaga aaattgaaat agaaaacttt ggtccatgga caataaaaca aaatcagttt
     8881 gccgttcaac caccaataga attccttgaa aacatgtata ccatccgcat tcaccttgat
     8941 gatacagatg aaaacaacgg tgcacttaga gtaatcccaa aatcgcatgt aaaaaagata
     9001 tacagacctg aaaccatcga ctggactaca gaaaacgaaa gcatttgcaa ggttgccaaa
     9061 ggaggaataa tgatcatgaa acctttaatt ctgcatagtt caggaagaac cacaaataat
     9121 aataaaagac gtgttatcca tatcgaattt tcaaacactg aacttcctga cgggttgcat
     9181 tgggcagagc gaatgaatgt attaaattaa acgctcaatc gaaacattca caacctaata
     9241 ctaataaaca accaaccccc aatattcaac tcactatacg tatcttgtaa atggggtgag
     9301 tgggtgagta tcgtgaagcg tgagtcgtga ctcgtgaata taaataataa gtaactacaa
     9361 tatagatttc ctccttcaaa ccttcaaagc tttaatcctt cgaatctttg aatcttcaaa
     9421 ccttcgaatc ttcaatcctt caatccttca aaccttcaac ccttcgaatc ttcaattctt
     9481 caaaccttcg aatcttcaat tcttcaaccc ttcaaatctt caaatcccgt acctttgccc
     9541 cgtgaaaccc aacgtcaagg cacacattta tgtgcttgca gcaaacatta tttatggtat
     9601 aaattatagt gtcggaaaag tggcacttaa agaaatgtcg cctctagcaa ttgtagcatt
     9661 tcgtgtctgt tgcgcctttc tggtgttttt tgctgcacat cagattttta tcaaagaaaa
     9721 tgttgaccgc gcagatcaca agaaatttat cctcgcaagt ctttttggtg ttgccgtaaa
     9781 tcagatgttg tttttcaccg gactcagtct taccagcgaa atgcatgcct ctctgatcat
     9841 gattactacg ccattgttgg tgctcatcat ggcatgggta attttgcgcg atcccattac
     9901 catcaccaaa tcaattggca ttttgtcggg cgccgctggt gttgcattac ttattatttc
     9961 gggaactgtt gccgacaatt caacagcttc ggcattcggc gatctgtgca tttttatcaa
    10021 tgccacttcc tatgccatat ttttagtgat cgcaaaacca ttgatgaaaa aatatgcccc
    10081 attgacactg gcaaagtgga ttttctttta tggcatgttc atggttattc ctttcggctt
    10141 caacgatctt caacaattat ctttcggtga aatatctaca caggcctggt tagcactttc
    10201 agtggttgtt ttcggggcaa ccgtgctggc atatttattc aatattctcg gattgaggta
    10261 tggcagccct gcactggtga gcatttacat ttatacccaa cccattatcg caaccatggt
    10321 agcccttatg cttggaacag acagcctcac ccttccaaag gttatttctg ccatcctggt
    10381 atttgccggt gtcgcattgg tatcgcgcag ttctatagcc attcctgcag cagaacctga
    10441 agccaaattg aaataagcta actttacaca aaattttgtg tatgctcatt tcaatgacag
    10501 gatatggtag aacaacattt gcgttgaacg gaaagaatat tgtgctggaa gtaaaaacac
    10561 tcaatagcaa atctttcgac ctcaacctgc gtatcgcccc tctgtttaag gaatatgaaa
    10621 tggagatccg caacctgctg agtaaatata tcatcagagg aaaaactgat tgcctgctta
    10681 cttacgaaca aatggatgaa aaggcaggag agataaacat taatgctatt aaggaatatt
    10741 atcgtcagat aaaatccatt tccgacaaca tgggtcttga acaggctaat ataatggacc
    10801 ttgtttttcg cttgccaaat atcaccacct catcaaacga accacttacc gacgagcagt
    10861 gggagatggt aaccaaccat atcgataata cctgtggtca gctcacaaaa ttccgcacag
    10921 tcgaaggtat taatctcgaa caagatctca tcctgcacgt caacctcatt cacagcgctt
    10981 taatagaagt agaagcactc gatccgaaac gcatggcaca gcagcgcgaa aaaatgcaca
    11041 acgatctgcg ctcttatctc gcatcggaaa atattgatat gaaccgtttc gaacaggaag
    11101 taattttcta tctcgaaaaa atggatatca ccgaagaagt gattcgcctc cgctcacatt
    11161 gcgaatattt tatgcagact attgaagata aagcgcagga aaaaggtaaa aaactcggat
    11221 tcattgctca ggaaatgaat cgtgaaatca atactatagg cagcaaagca tatcatgccg
    11281 acatgcaacg caaggtagta gagatgaaag atgaattgga aaaaataaaa gagcagctca
    11341 acaacgtttt ataatgagtg gcaaactgat tatcgtaaca gctccatcag gcgcaggaaa
    11401 aacgaccatc gtcagacacc tgttagcggt agagcctttg cttgcatttt cagtttctgc
    11461 aaccactcgc cagaaacgcg attatgaaaa agatggcgtt gattaccatt ttttgagtgc
    11521 cgatcaattc cgcaaacaaa ttgaggaaaa tgcttttgtg gagtgggaag aagtttatcc
    11581 cggcaaattc tacggcaccc tgcgcagtga agtagaacgc atgtgggcag caggaaaaca
    11641 tatcatcttc gatgtggatg tgaaaggtgc cctcagctta aaatccaagt ttccggaaaa
    11701 aacactgagt ctgtttatca gtccgccgag tgtagaaatt ctagcccaac gcctcatcga
    11761 cagaggcaca gaaactccct caaccctgca aacacgcctc gaaagagtgc gttatgagct
    11821 cactttcagc ccgaaattcg atcatgttat tgtgaacgac gtgctggaaa cggcgaaaac
    11881 agaggctcag cgcctcgtgc ataacttttt gaacatggca tgagcgccta accccatttt
    11941 tctgttacat ttacatcacc aaccttaagg catggttacc agcattactc aaggcgtaaa
    12001 ggtcagcgta gaaactttct accaacaaga atttgccaat aaaggcgaat atgtgttcgc
    12061 atatcgcatc actatcgaaa acaaaagcga gcataccgtg caattacttc gtcgccattg
    12121 gtatatctac gatggtatcg gaacctggcg tgaagttgag ggcgaaggtg ttgttggtga
    12181 aactcctgtt atcgctcccg gtcatatcca tcaatacgtt tccggatgtc acctgctggc
    12241 accggcaggc aaaatgcatg gctcctacct catgatgcgc accctcgacg gcatgtacat
    12301 cgatgtcacc atcccggaat ttcatatggt ggcacctttt gtgctgaa
//
LOCUS       DAOQHY010000101        12178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2555_length_12178_cov_5.494267, whole genome shotgun sequence.
ACCESSION   DAOQHY010000101 DAOQHY010000000
VERSION     DAOQHY010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12178
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2555_length_12178_cov_5.494267"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..1007
                     /locus_tag="PLL28_09270"
     CDS             297..1007
                     /locus_tag="PLL28_09270"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNF69552.1"
                     /translation="MQKLILAAVMVFTIATAFAQVDSAKTTTTVLEKEDPSRDRFLIN
                     VHWDGWMGAPDSMKIKGLSSGIGLHYMYDVPLKDDNISFAIGAGVSWSNYYNNSYFTY
                     DTSSYTVPVKFADTVQYSKNKFVLNYVEVPLEFRFRTDDKNGNRFKAAIGFKAGYAFS
                     NHTKYVGDDYISGSGDEIKFKQYRVKNVSTLQYGPTLRLGYSKINIEAYYGLAPIFTD
                     GEGPTGNPITVGVSFNPF"
     gene            1076..2059
                     /gene="meaB"
                     /locus_tag="PLL28_09275"
     CDS             1076..2059
                     /gene="meaB"
                     /locus_tag="PLL28_09275"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF006958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNF69553.1"
                     /translation="MAQLDLTTEQWLTGIRNGDRVLLSRAITLIESAVPAHREQALAL
                     LSACYPLSGSAMRIGITGSPGAGKSTTIENLGLQMLADNKKLAVLAVDPSSRISKGSI
                     LGDKTRMQQLSREASVFIRPTASGEHVGGVAAATRETIILLEAAGFDTILIETVGVGQ
                     GETAIYDMTDLFILLLIPGAGDELQGIKRGIMELADIILINKCDGDNVERAKLAAAEV
                     RRAIHLFQSKTSEWPVCVELISGLEGRGIPELRHQIDKYFELTKHNGYLESNRIRQRE
                     SWFQDQLHYAILRSVSEHPQYHEWLKSAEADIQTGQLLPSLAVHTLLKKLF"
     gene            2172..2366
                     /locus_tag="PLL28_09280"
     CDS             2172..2366
                     /locus_tag="PLL28_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69554.1"
                     /translation="MNFNPNLKIDEIIVRYALLLVVGIVAGVLHAYWMIPVVMGIFLT
                     AVLGWCPVKAMMKGKKVTAS"
     gene            2798..3943
                     /locus_tag="PLL28_09285"
     CDS             2798..3943
                     /locus_tag="PLL28_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HNF69555.1"
                     /translation="MDLSYILNILGEERDQYFDAVTPPIVQTSNFAFKTVADFRDAIN
                     TEQQHHVYTRGNNPTVEIVRKKIAALEGTDDCLVFSSGVAAIAAILLHTLRSGDHVIC
                     VKSPYSWTQYLLTEVFPEYNITTTFIDGREMSQFEQAIQSNTKLIYLESPNTFWFELQ
                     DLEAVSKLARKHHIFTAIDNSYAAGIYQHPHKLGVDFVIHTATKYLAGHSDVVAGAVC
                     GNSEHIAGMFRRVFMGFGAVPSPHDAWLLLRGMRTLPIRLEKSASNAEMIVEHFMGHP
                     KIERIIHPFHPSHPQFALAQKQMTRGCGLFAMILKTENKTQVEAFCNSLERFLMAVSW
                     GGYESLIVPACIKIGDDYPAGFVRFYAGLEEANVLIADIEKALQYID"
     gene            complement(3944..4582)
                     /locus_tag="PLL28_09290"
     CDS             complement(3944..4582)
                     /locus_tag="PLL28_09290"
                     /inference="COORDINATES: protein motif:HMM:NF018766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HNF69556.1"
                     /translation="MLKTLRLSRYHYLLCCTAFSMLLLLARMYHTNTLEYRFMAINLF
                     LAWIPYGVAKLMQNIPIGKWRPLIIVLAFVWLIFFPNAPYMITDIFHLSEFDAMPKWY
                     DLIMLLSFSWTGLLLGFFSLRKVLHRINAGNNFPQMIVSFIIFFLCGAGIYLGRYERW
                     NSWEIITQPRILISDFLGLLAQDAVMFQMATLSFVFATFFTMIFYFVPEETT"
     gene            complement(4585..4938)
                     /locus_tag="PLL28_09295"
     CDS             complement(4585..4938)
                     /locus_tag="PLL28_09295"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNF69557.1"
                     /translation="MTKKRNEIKSFADAFRGIGILFRRERHAQIHLVVAIAVVFAGFY
                     FSITDIQWLVIALTIAMVFTAEGLNTAIEKLSDAVHPERSEKIRDVKDIAAGAVLISC
                     IIAVIIGCCIFIPYL"
     gene            complement(4919..5956)
                     /locus_tag="PLL28_09300"
     CDS             complement(4919..5956)
                     /locus_tag="PLL28_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69558.1"
                     /translation="MTSGKKPMIAHVWQLFIRWVELYTWRFILSLLLITFSIIVVWEP
                     FWFVSALDALRIPYAVFLVAFVMFFFLRNNYALASAGGIALLIILPGIWPYFKTANEI
                     PAEAKKEKLEIVESDLTVLHFNVKENNKKIETVAEAAVQSGAIIVSMQELKPISFPKV
                     DTIMRSIYPYVYSELDYPGFGMALYAKVPFTSTETLEFNGFPVLHCTVEIDGTEIHLL
                     AATTSTPTNDKDAQLQAKQFVFMSELVLALQGPLFLMGDMNSVPWSEGIEGLLKKTQL
                     KDSRKDLSATYPAQSPLQIPIDYIFHSKEFTCNKFSTVGGTTSNHLGLMGYYTLRSTK
                     KSKIQDDKKAQ"
     gene            complement(6101..6391)
                     /locus_tag="PLL28_09305"
     CDS             complement(6101..6391)
                     /locus_tag="PLL28_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNF69559.1"
                     /translation="MIEGLNKEFDNRVRLGIMSILMVNSDVDFNSVKNLLQLTDGNLA
                     SHMSALEKAGYVEVNKAFVGKKPRTTYAVSESGKKAFQEHLNALEKLIKMGK"
     gene            complement(6388..7020)
                     /locus_tag="PLL28_09310"
     CDS             complement(6388..7020)
                     /locus_tag="PLL28_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69560.1"
                     /translation="MTDDNQQHALEDLKEIRAMMERSSRFISLSGLSGISAGIIAIIG
                     AFIGGWYINKNIDYVEGNIARENVRTEDVVFLLLLAGVILFLAIGAAIYFAIRKARLN
                     NLPIWNKAAELTLVNLLLPLGTGAIFCLILFTYGLYILIAPATLVFYGLALLNASKFT
                     LSEIRYLGVSEIVLGLIAMVIPGYSILFWALGFGLLHIVYGAVMYFRYER"
     gene            7138..8403
                     /locus_tag="PLL28_09315"
     CDS             7138..8403
                     /locus_tag="PLL28_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69561.1"
                     /translation="MTGVQLVYGQLQCDNDTSYLRSLIDLKTGLYLGYQGGLYPDGSN
                     TMPYSHYADGMNISRQVFPLNADGAVDLVDGKVGFICLGASTAGNAFNHFKTVCEEDP
                     AVNPCLKFANCAVGAKGLEVMIDTIEDAWYWDDEVMPDLDYAGITRHQVEIIWVMVTS
                     RIDTILIWPYQPQEVANKYEILMQVLLKKFPNLKQVYLSGFNYGGYADPTKEFYDMIM
                     EPSSYWNNWSVKFLIERQIEDDPGLDYKAPDRVAPWLGWGPHIWADGLRANTTDHLRW
                     VCEVDYKPDGGGYHMSNTGKDKMGKLIYDYFINSPIAADWFKYGARWTSCDPDFKVEE
                     PSTEATLTISPNPAGNDALLELDRWADLPVEVTIYNAMGQPLSNLNFCPSNSYIAYPV
                     SLAGLPTGTYYIVARQTGLRVMSLSFLKQ"
     gene            8495..9826
                     /locus_tag="PLL28_09320"
     CDS             8495..9826
                     /locus_tag="PLL28_09320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69562.1"
                     /translation="MRKLILACTLLAATTIAQPVFGQLNCANDSTGLIPLVDLGTDYY
                     AGIYQGGLYPGGSNIMPSGHMINGLKIMKKLKPLDTAGVVNYATGKIVLGGFGASTVG
                     GPFNHMIQLMKDYNDLNPCMKAVNTANGSDGIEAMYIGNEEYFDYIKIYKLAEKDLTP
                     IQVQVGWLMHSSRIDSNGTDPIGYVDSLSKRFLIALNTMLYYYPNLKVVYLSGFPYGG
                     YADPMKALYHVIKEPSSYHHNFAMKSLIERQITGDPALKYKGAGIQVPYLAWGPNIWA
                     DGKNPRELDSLTWNCETEFAIDGGGYHLTNEGKDKLANILLQFFRTDTLSEGWFMDGP
                     KWASCGAGRLADGSIILPEEPTILTKDEITVFPNPSSGQYYVDFDEVLTEAIQVKVVN
                     QVGEVVLEESYGGIQPFSFYQFNLSDQPAGIYYFEVVANGEHFSQPIILNH"
     gene            9831..11174
                     /locus_tag="PLL28_09325"
     CDS             9831..11174
                     /locus_tag="PLL28_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69563.1"
                     /translation="MHIRALQPVNQYITLQRISAIALILCCFSATAQINCENDTSFHI
                     PIVDLQTDYFGVHQGGLYPDGLNTMPAAHADSGIAIAESLLPINYDGQEDTSYGKFVF
                     LGLGNEAAGKIFNKFITQHDDLGLGDSCMRIVNACMEDYTLHDMYGADASDAYWNDVN
                     DYLQSQNLKKKQVRAVWLMTTNYDDTLITEDAYIDSVKNTYIEVIRKMKKQLPNLSLL
                     YISGLPYGGYADTMFDGHEFFEEPIPYLNDFAIKAVIDAQINGDTSLIYSGEDADAPW
                     IAWGPNVWADGRNLRAYDNLRWLCPGDYASGDNGIMLSGTGQTKVADRLYTFLTSEPT
                     TLPWIYGLPYDCFTEPDFVEPEDSVIVPEDEILWITQNPVKGVIKFVVNVETNDKAQI
                     YVFDMIGNQIVEGVFNKIEPGRVLSVNVTQNARGLYILSVFVENKVYNKVFYLDN"
     gene            complement(11183..>12178)
                     /gene="thiL"
                     /locus_tag="PLL28_09330"
     CDS             complement(11183..>12178)
                     /gene="thiL"
                     /locus_tag="PLL28_09330"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNF69564.1"
                     /translation="LIDLLTQNTEIKHAATLKGIGDDAAVVDHSHLVSVISTDMLVEG
                     VHFDMMYTPLQHLGFKAIAVNVSDICAMNATATQVLVSIAISNRFSVEALEALYEGIH
                     HACQYFDVDLIGGDTTTSLKGLVISVTVIGEANESDIAYRNGAKPGDLICVTGDLGAA
                     YCGLQLLEREKQIYLENPEIQPDLENKKHIVGRFLRPEARKDIITYFKEHGIKPTSMI
                     DISDGLSSEILHICKQSACGAIIYEDAIPIHEETYQQATKFNIDPLVCALNGGEDYEL
                     LFTVDESMGEKIAQHAGISVIGKITDKASGTFLKTRNENLFKIEAQGWNSFPDQQ"
BASE COUNT         3604 a         2522 c         2561 g         3491 t
ORIGIN      
        1 ccggtttcaa aaatctcgct ccattgcgct gttgccgttc caagagtgca agcaaatgtt
       61 aaaagtagaa ctgtctgttt catcgggtgt caattttaac taaccaaatg tatccttttt
      121 caggggaaag tggcggcata aataatatcg tgggattacc cggttaaacc aaagtaaggt
      181 gccataccaa gcgcaaattt tagacgttat taggcttcgg acgtgcataa aggccatgtt
      241 aacgactgaa aatggttaat tttgccccat acaataaact tacaaagcaa agcaacatgc
      301 agaaattgat attagcggct gtgatggttt ttacgattgc cacggcattt gcgcaagtag
      361 attcagcgaa aacaacaaca acggttttgg aaaaagaaga cccttcacgt gacagatttc
      421 taatcaatgt tcattgggat ggttggatgg gtgctcctga ctcaatgaaa ataaaaggcc
      481 tttcaagtgg tatcgggttg cattatatgt acgatgtgcc attgaaagat gataatatca
      541 gcttcgctat tggagcaggt gtttcatgga gcaattatta caacaattca tattttactt
      601 acgacacatc atcatacact gtgcctgtaa aatttgctga tacggtacag tattctaaaa
      661 ataaatttgt actcaactat gttgaagtgc cattggaatt ccgtttccgc accgatgata
      721 aaaatggcaa cagattcaag gctgctatcg gtttcaaggc gggatacgca ttcagtaatc
      781 ataccaaata tgttggcgat gattatatca gtggatccgg cgatgagatc aaattcaaac
      841 aatatcgcgt taaaaatgta agtaccttgc aatacggacc aacgctccga ctcggctaca
      901 gcaagatcaa tatcgaagcc tattacggat tggcgccgat attcaccgat ggcgaagggc
      961 caacaggaaa ccctataaca gtgggtgttt catttaatcc attctgataa gcaattaaga
     1021 atattttgtg gtccctccgt gttttacgcg gggggatctt atttttatac cggtaatggc
     1081 acaactcgat ctcacaacag agcaatggtt aacaggtatc cgcaatggcg acagggtatt
     1141 attatcccgc gcaattacgt tgattgaaag cgctgtacct gctcatcgag agcaagcatt
     1201 ggccttattg tcggcatgct atcctctcag tggctctgcc atgcgcatcg gtattacagg
     1261 tagccccgga gcaggtaaaa gcaccacaat tgaaaatttg ggattgcaaa tgcttgccga
     1321 caataaaaaa ttggcagtac tggcagttga ccccagcagc cgcatcagca agggaagtat
     1381 tctgggcgat aaaacgcgta tgcagcagct atctcgggag gcctcagtat ttatcaggcc
     1441 aacagccagc ggcgaacatg tgggtggtgt ggcagcggct acgcgtgaaa ccatcatctt
     1501 gctggaagct gcaggatttg atacgatatt gatagaaacg gttggagtag gtcagggcga
     1561 aactgccatt tacgacatga cggatctttt tatcctcttg ttgattccgg gtgcaggcga
     1621 tgaattacag ggtattaaaa gaggtatcat ggaactggca gacatcatcc tcataaacaa
     1681 atgcgatggc gataatgtag aacgcgcaaa actggcagca gcagaagtga gaagagctat
     1741 acatctgttt cagtcgaaga catctgaatg gcctgtttgt gttgaattga tttcaggtct
     1801 tgagggtagg ggaatccctg aattaaggca tcaaatcgac aagtattttg agttgacgaa
     1861 gcataatgga tatctcgaat ccaatcgcat ccggcagcgt gaaagctggt ttcaagatca
     1921 attacattat gctattctgc gctctgtgag tgagcatccg caataccacg aatggctcaa
     1981 atctgctgaa gctgatatcc aaaccggaca attgctccct tctttagcag tgcacacttt
     2041 gctgaaaaag ctattttaag cctgaaaaat gttaatcgct gtcggctgac taaaatcacc
     2101 gatttgcctg atcgaagtca ttacttgtgg tttgtggtag aatgatcttt accaacgtaa
     2161 tcacaatgta tatgaacttt aatccaaatc ttaaaatcga cgaaatcata gtaaggtatg
     2221 ccttacttct agtagttggc atcgtagcag gcgttttaca tgcctactgg atgatacctg
     2281 ttgttatggg aattttcctt actgctgttc tcggctggtg tccggtgaaa gcaatgatga
     2341 aaggaaaaaa agttactgcg tcgtaattca tcgttctgtt aaaacgacgc ttgacacgat
     2401 caatcgtgcc atacggttgt cttttctttg agtatgcctg aaaaaatagc ccgcctctcg
     2461 gcgggttatt ttatttatgg gaaatgagta ataccaatca aatccaacgt cattagtctc
     2521 acctgggaat gtaaagtcgt gactcgtgaa tcgtgactcg cgggctggtc tcgccgagca
     2581 gtcgggtcgg gaatggtaaa taagcaaact caccactcac cactcaccca ctcacccacc
     2641 cgccagaggc gaggcaagct cacaattcac gattcacata ccacactcac gattcacgac
     2701 tcacgattca cgactcacga ctcacccact cacccacccg ccagaggcga ggcaagctca
     2761 cccatttatt acttttacag tccaataacc caccatcatg gacctgtcat acatcctcaa
     2821 tatcctcggc gaagaacgcg atcagtattt tgatgcggta acacctccga tagtgcagac
     2881 cagcaatttt gcatttaaaa ccgtcgccga ttttcgggat gctattaata cggagcaaca
     2941 gcatcatgtg tataccagag gaaataatcc tacagttgaa attgtaagaa aaaaaatagc
     3001 agccttggaa ggtaccgatg attgtctggt gttcagtagt ggcgtggctg ccattgctgc
     3061 tatattattg catacacttc gtagcggtga tcatgttata tgtgtgaaga gtccttatag
     3121 ctggacgcaa tatttgctta ctgaggtatt tcctgagtat aatatcacga caacatttat
     3181 tgatggacgc gaaatgtctc aatttgaaca agcaattcaa tcaaatacaa aactcattta
     3241 tctcgaaagt ccaaatacct tctggttcga attacaagac cttgaagctg tttccaagct
     3301 tgcaagaaag catcatattt tcacagcaat agacaatagt tatgccgcag gcatttatca
     3361 gcatccgcat aaattaggcg ttgattttgt tattcatacg gcaacaaaat atcttgccgg
     3421 tcatagcgat gtggtagcag gtgcagtttg tggcaattcc gaacatattg caggcatgtt
     3481 tcgcagagta tttatgggat tcggtgctgt gccttcgccg catgatgcat ggttactttt
     3541 acgtggtatg cgcaccttgc caattcgatt ggaaaaaagt gcatctaatg ctgaaatgat
     3601 agtcgagcat tttatgggac atccgaaaat tgaacgcatc attcatcctt tccatcctag
     3661 ccatccgcaa tttgcacttg cacaaaaaca aatgacacgt ggttgcggat tgtttgccat
     3721 gattcttaaa acagaaaata aaacacaagt tgaagcattt tgtaatagcc tcgaacgctt
     3781 cctcatggct gtcagttggg gtggatatga atcattgatt gtgcccgcgt gtattaaaat
     3841 cggcgatgat tatcctgccg gatttgtgcg gttctatgcg ggacttgaag aagcaaatgt
     3901 gttgattgct gatatcgaga aagcattgca atacatcgat tgatcaagta gtttcctcgg
     3961 ggacaaaata aaatatcatc gtaaaaaaag tggcaaagac aaaactcaat gttgccattt
     4021 gaaacatcac ggcgtcttgt gcaagtaatc caaggaaatc ggaaattagt attcggggtt
     4081 gagtaatgat ttcccagctg ttccaacgtt cgtagcgacc taaataaatt cctgcgccac
     4141 acaggaaaaa tatgataaag gatactatca tttgcggaaa attattcccg gcattgatgc
     4201 gatgcaacac tttgcgcagt gagaaaaatc cgagtaacaa tcctgtccat gaaaaagata
     4261 acagcatgat caggtcatac catttcggca tggcatcgaa ttcggaaagg tgaaatatat
     4321 ccgtgatcat atatggtgca ttcgggaaaa atatcaacca tacgaatgca agcacaatta
     4381 ttagcggacg ccattttcca ataggaatgt tttgcataag ttttgctacg ccatacggga
     4441 tccatgccag aaaaagatta atcgccataa atctatactc cagggtgttg gtgtgataca
     4501 tcctggccaa caacagcaac atcgaaaatg ccgtgcagca caacaaataa tggtagcgac
     4561 ttaatcttaa tgttttgagc ataattataa atatgggatg aatatgcagc aaccaatgat
     4621 tacagctata atgcaagaaa tcagaaccgc tccggcggca atgtctttta catcacgtat
     4681 tttttcactt ctttcgggat ggacagcatc agacaatttt tcaattgctg tgttgagacc
     4741 ttctgcagta aaaaccattg caatagtcaa ggcaatgaca agccattgta tatcggtaat
     4801 ggaaaaataa aaacctgcga ataccacggc aattgcaact accaaatgta tttgcgcatg
     4861 ccgctctcgc cggaataata ttccaattcc ccgaaaagca tcagcaaaac tttttatttc
     4921 attgcgcttt tttgtcatct tgtattttag acttttttgt tgagcgcaag gtataataac
     4981 ccattagtcc gaggtgattg cttgtagtgc cgccaacggt actgaattta ttgcatgtaa
     5041 attccttcga atgaaaaata taatcgatgg gaatttgtag tggcgactgg gcagggtagg
     5101 tggcagagag gtctttgcga ctgtctttta attgcgtttt tttcaacaag ccttcaatac
     5161 cttcactcca tggaacggaa ttcatatcgc ccattaaaaa taacggtcct tgcaaggcga
     5221 gcactagttc cgacataaat acaaattgtt tggcttgcaa ttgtgcatct ttgtcgtttg
     5281 ttggtgtact ggtggttgcc gccagcaaat gtatttcagt gccgtcaatt tctaccgtgc
     5341 aatgtagtac aggaaatcca ttaaattcta gcgtttcagt tgatgtaaaa ggaacctttg
     5401 cgtacaatgc cataccaaaa ccgggatagt ccagttcgct ataaacatag ggataaatgc
     5461 tgcgcataat ggtatccacc ttaggaaaag aaataggttt taattcttgc attgacacga
     5521 ttatagctcc ggattgaaca gctgcctctg caacggtttc tattttttta ttgttttcct
     5581 tgacattaaa atgcaacacc gtaagatctg attctacaat ttccagcttt tctttttttg
     5641 cctctgccgg tatctcattc gcagttttaa aatatggcca gattcccgga agtatgatca
     5701 ataatgcgat gcctcccgct gatgccaaag cataattatt ccgcagaaaa aagaacatta
     5761 caaaggctac cagaaaaaca gcatacggta tgcgcaaggc atccaatgcc gatacaaacc
     5821 aaaagggttc ccataccaca ataatgctga aagtgattag caggagcgat aaaataaatc
     5881 gccacgtata tagctccacc caacgtatga acagctgcca cacatgggct atcatcggct
     5941 tctttccgga ggtcatggtg caaatataca acttactttg aaattcaaag tagaaattgc
     6001 acgggaaagc gttatcacat gaaataatgc tgcaaaaagt gcgccgaatt tgacctatac
     6061 gccttgcaat ccttaagggt tcaacattcg aggcgggtct ttatttaccc attttgatca
     6121 acttttccag ggcattgagg tgctcctgaa aggccttttt gccgctttcg gaaacagcat
     6181 aagtggtacg tggtttcttg cctacaaatg ctttgttcac ttctacatag cccgcttttt
     6241 cgagtgccga catatgactg gcaagattcc cgtcggtgag ctgaagaagg tttttaacgg
     6301 aattaaaatc tacgtcgctg ttcaccatga gaatactcat aatgcccaat cgtacacgat
     6361 tatcaaattc cttatttaat ccttcaatca tctttcgtat cggaagtaca tcactgcacc
     6421 ataaacaata tgcaaaagtc caaatcctaa agcccaaaat aatatcgagt atccgggaat
     6481 aaccatagca atgagaccga gaacaatctc tgaaacgcct aagtacctaa tctctgagag
     6541 tgtgaatttg ctggcattta ataatgctaa tccataaaat actaaggttg caggagctat
     6601 taaaatatat aatccgtaag taaataatat cagacaaaat atagcaccgg taccaagcgg
     6661 taataataaa ttcaccaatg taagttcagc tgctttattc caaattggta aattgttgag
     6721 tcttgctttt ctgatggcaa aataaattgc tgctccaatt gcaagaaata aaattacacc
     6781 ggccagtagc aacagaaaaa ctacatcttc tgtgcgcaca ttttctctgg ctatatttcc
     6841 ctctacataa tcgatatttt tgttgatata ccaacctccg ataaaggctc caattatggc
     6901 aatgattccg gcactaattc ctgataaacc ggaaagtgaa atgaaacgcg acgaacgttc
     6961 catcattgcc ctgatctcct tcaggtcttc aagtgcatgt tgctgattat catcggtcat
     7021 agtaaagcgc tttgaccagc aaagttaccc aatgcccggc aggaaatcat aattcactat
     7081 ttttgaaacc ttatgcaagc attgcgactc ctccggcact taaacctgtt gttgcttatg
     7141 accggtgttc aactggttta tgggcaattg caatgcgata atgacacctc gtatctccgc
     7201 tcgctgattg acctcaaaac aggtctttat cttggctatc aaggcggtct gtatcccgat
     7261 ggctcgaata caatgcccta ttcgcactat gctgatggaa tgaatatcag tcgccaggtt
     7321 tttccgctca atgccgatgg tgctgttgat cttgtagatg gtaaagtagg ttttatttgc
     7381 ctgggagcat caactgccgg aaatgcattt aatcatttta aaacagtttg cgaagaagac
     7441 cccgctgtaa atccgtgtct caaattcgct aattgtgcag taggcgccaa agggcttgag
     7501 gtaatgatag atactattga agatgcatgg tattgggatg atgaagtgat gccggaccta
     7561 gattacgccg gaatcacgcg ccatcaggtt gaaataattt gggtaatggt gacatccaga
     7621 atcgatacta ttctgatatg gccttatcaa ccacaggaag tagccaataa atatgagata
     7681 ctgatgcagg tgttgctgaa aaaattcccg aatctcaaac aggtttatct cagtggcttc
     7741 aattatggcg gttatgccga tccaaccaaa gagttttatg atatgatcat ggaaccgtcc
     7801 agttattgga ataattggtc ggtaaagttt ctcatcgagc gacaaatcga agacgatccc
     7861 ggtcttgatt ataaggctcc tgacagggta gcaccatggt taggctgggg ccctcatatc
     7921 tgggctgatg ggctaagagc caatacgact gaccatttgc gttgggtttg cgaggtagat
     7981 tacaagcctg atggcggcgg ctaccatatg tcgaataccg gtaaggataa gatgggaaaa
     8041 ctcatatatg attatttcat caattcaccc atagcagccg attggtttaa atatggtgct
     8101 cgctggacta gttgtgatcc cgactttaag gttgaagaac cttctactga ggcaacttta
     8161 actattagcc caaacccggc cggaaacgac gctttattag aactcgatcg ctgggccgat
     8221 cttccggttg aagttactat ttacaacgca atggggcagc cgctttcaaa cctaaacttt
     8281 tgtccatcaa atagttatat cgcttatcct gtttccttgg caggcctgcc taccggaaca
     8341 tactacatcg tagcacgcca aaccggatta cgtgtgatgt cgctcagctt tctcaagcaa
     8401 taattatttt tgacgccacg caactaggaa gcccatttgg ctttctgtat ctttgacaac
     8461 tttttttaca gaacgatacc aactttatta atccatgaga aaattaatac tcgcatgcac
     8521 actactggca gcaactacaa ttgcacagcc ggtttttgga caattgaatt gtgctaatga
     8581 ttcaacaggt cttattcccc ttgttgacct tggcacagat tactatgccg gaatttacca
     8641 gggaggtctc tatcccggtg gttcgaatat catgcctagc ggtcacatga tcaacggttt
     8701 gaaaatcatg aaaaaattaa aaccactcga tactgccggt gttgtaaact acgctacagg
     8761 taaaattgta ttgggtggat ttggtgcttc aaccgtaggt ggaccattca atcacatgat
     8821 ccagttaatg aaagattaca atgacctcaa cccatgtatg aaagcagtaa atactgcaaa
     8881 cggttctgat ggtatagagg caatgtatat cggcaacgaa gaatatttcg attacatcaa
     8941 aatttataag cttgctgaaa aagatcttac tcctattcaa gttcaggttg gttggttgat
     9001 gcacagctct cgtatcgact ctaatggtac tgatcccatc ggttatgtag attcattgag
     9061 caaacgcttc ctgatcgcac ttaatacaat gttgtattac tatccaaacc tgaaggtggt
     9121 ttacctcagt ggtttcccat acggaggata tgcagatcca atgaaagcac tttatcatgt
     9181 aattaaagaa ccttcaagct atcatcacaa ctttgcaatg aagtcgctga tcgaacgtca
     9241 gatcaccggc gatcctgcat taaaatataa aggcgccgga attcaagtgc cttatcttgc
     9301 atggggacca aatatttggg ctgatggaaa aaatcctcgc gaactggata gcttgacctg
     9361 gaattgtgaa actgaatttg ccatcgatgg tggtggatat cacctcacca acgaaggaaa
     9421 agataaactc gcgaatattt tattgcaatt cttccgcaca gacaccttgt cggaaggttg
     9481 gtttatggat ggtccgaaat gggcgtcatg cggcgcaggt cgtttagcag atggtagcat
     9541 tattttgcct gaagaaccta caattttaac caaagatgaa atcacggtat tccctaaccc
     9601 ttcatcaggt caatattatg ttgattttga tgaagtgctg acagaggcaa tacaagtaaa
     9661 ggttgtaaat caggttggtg aagtagtgct ggaagaatct tatggtggaa ttcaaccatt
     9721 ctctttctat caatttaatt taagcgatca gcctgcaggt atttattatt ttgaagttgt
     9781 ggccaatggg gaacattttt cacagccaat tattttgaat cattaagcat atgcatattc
     9841 gggcattaca gcctgttaat caatacatta cattacaaag gataagtgca atcgcgctta
     9901 tcctttgttg tttttctgcc acagctcaga taaattgtga aaatgatact tcatttcaca
     9961 tacccattgt agatcttcaa acagattatt ttggtgtaca tcaaggtgga ttatatccgg
    10021 atgggttaaa tacaatgccc gccgcacacg ccgattcagg aattgccata gccgaaagtt
    10081 tattgcctat taattatgat gggcaggaag atacttcata cggtaaattt gtttttctcg
    10141 ggctcggtaa tgaggccgca ggaaagattt tcaacaaatt tattacacaa cacgatgatt
    10201 tgggtttagg tgattcatgt atgcgcatcg taaacgcttg catggaagat tacacccttc
    10261 acgacatgta cggcgccgat gcatctgatg cttattggaa tgatgtgaat gattatttac
    10321 aaagccagaa cctcaagaaa aaacaagttc gcgcagtatg gctgatgacc acgaattacg
    10381 acgacacact gattactgaa gatgcctata ttgattcagt gaaaaatacg tatatcgaag
    10441 tgatacgtaa aatgaaaaag caattaccta atttgtcttt gctgtatata tccggattgc
    10501 catatggagg atatgctgat accatgtttg acggtcacga attttttgaa gagcccattc
    10561 catacctcaa tgattttgca attaaagcag ttattgatgc acaaataaac ggtgatacct
    10621 cattaattta ttccggcgag gatgccgatg caccgtggat agcatgggga ccaaatgttt
    10681 gggctgatgg aagaaatctt cgtgcatatg ataatctgcg atggctttgt cccggtgatt
    10741 acgccagcgg tgataatggc atcatgctaa gcggtacagg tcaaacaaaa gttgccgatc
    10801 gattgtatac atttttaaca agcgagccta caacactgcc ttggatttat ggtttgcctt
    10861 atgattgttt caccgaaccg gattttgttg aacccgaaga ctctgttatt gttcctgagg
    10921 acgaaatact ttggattaca caaaatcctg tgaaaggtgt gatcaaattt gtggtcaatg
    10981 ttgaaacaaa tgataaagca cagatctatg ttttcgatat gataggcaac caaatagtag
    11041 agggcgtctt caataaaatt gaaccgggta gagtgttgag cgtaaatgtg actcaaaatg
    11101 ctagaggatt gtacatcctc tcggtatttg tcgaaaacaa agtctacaac aaggtgtttt
    11161 atcttgataa ttgattcaac atttactgtt gatcaggaaa tgaattccat ccctgtgctt
    11221 ctattttgaa caaattctca ttccttgttt taagaaatgt accgcttgct ttatctgtaa
    11281 tttttccaat cacagatatt cctgcatgtt gtgcgatctt ttcacccatc gattcatcaa
    11341 cggtaaataa tagctcataa tcttcaccgc catttaatgc gcataccaat gggtcgatgt
    11401 tgaattttgt agcttgctga taggtttctt cgtgaatggg aatggcatcc tcataaatta
    11461 tcgcgccaca agcactctgc ttgcagatat gtaatatctc tgatgataaa ccatccgaaa
    11521 tatcaatcat ggatgtgggt tttattccat gttctttaaa gtaagttata atgtcttttc
    11581 tcgcttccgg acgaagaaac cttccaacaa tatgtttttt attttccaga tcgggttgaa
    11641 tttccggatt ttctaaataa atctgttttt ctcgttcaag taattgcaaa ccacaatagg
    11701 cagcaccaag atcgccggtg acacaaatga gatctcccgg tttggcgcca ttgcgatagg
    11761 cgatatcgct ttcattcgct tcgccaatta ctgttaccga tatgacaagt ccttttaaac
    11821 tggtagttgt atcaccgcca atcaaatcaa catcaaaata ctggcaagca tggtgaatgc
    11881 cttcatataa tgcctccagt gcttccacag aaaagcgatt ggagatggct atcgatacca
    11941 gtacttgtgt agctgttgca ttcatagcac agatatcact tacatttacc gctatggcct
    12001 taaagcccag gtgctgaaga ggggtgtaca tcatatcaaa atgcacacct tccaccagca
    12061 tgtcggtgct gataactgac accaaatgac tgtgatctac aaccgcggca tcatcgccaa
    12121 ttcccttcaa agtggctgca tgcttgatct cggtgttttg tgttaggagg tctattag
//
LOCUS       DAOQHY010000102        12140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2567_length_12140_cov_6.790153, whole genome shotgun sequence.
ACCESSION   DAOQHY010000102 DAOQHY010000000
VERSION     DAOQHY010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 12140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12140
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2567_length_12140_cov_6.790153"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1900)
                     /gene="recQ"
                     /locus_tag="PLL28_09335"
     CDS             complement(<1..1900)
                     /gene="recQ"
                     /locus_tag="PLL28_09335"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HNF69565.1"
                     /translation="MESMDAAKVLKRYFGYDAFRDQQEEVINHITSGHDTLVVMPTGG
                     GKSVCYQIPAMMLEGMAIVVSPLIALMKDQVDALVTNGINAVALNSSLTPDEQRIVTQ
                     KILRGEIKLLYIAPERLMGDEGRLIELLSTLKISLIAIDEAHCVSMWGHDFRPEYLKL
                     AKIREKLTRVPIIALTASADAQTRADILDKLNMPKAHISISSFNRANINYYIEQKRDA
                     YTAIINYIVQHQGQSGIIYTLSRANAEMLAERLTADGFEAAYYHAGMDAASRNTVQEA
                     FKKDKTNIIVATIAFGMGIDKPDVRYVIHYNLPKNIESYYQETGRAGRDGLPSDAILF
                     YSYGDLMQLRRFAEVEGNPERTRIMLDKLMRMNAFCVSNICRRTMLLDYFGEKYPESN
                     CGSCDVCLSRKTSFEATTIAQKALSAIVRTEERFGIHYLVDFLRGSRSEKIWKGHKQY
                     KTYGIGADISRDKWMQYFQELIDQQYVKRIAEGSYPRLLITEKGKRVLQGTEQVYFTL
                     SEERLMVENSNQIEYDSHLFDLLRSLRTRIAYRESLPPYMIMGDSTLQEMAAYLPVQI
                     EDLKDISGFGEVKIGKYGPQFIEVIRQHVKDHALMTKMSSKRKSKSPPKPRTKTRPGI
                     NTSEQETLE"
     gene            2095..3258
                     /locus_tag="PLL28_09340"
     CDS             2095..3258
                     /locus_tag="PLL28_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNF69566.1"
                     /translation="MKRILTVLIALLPLINFAQDVQFFQGTWEEVNAKAKAENKLIFV
                     DAYTYWCGPCKMMDETMFHNNKEVADYVNAHFIAYKVECEHDFGVEFARKYKVNVYPT
                     MLMFNSSGQLLEKHLGYNDVQSEFIETFRKVVNMDQTDVYAMDPAKIKMNWPEFYAKN
                     FKDANDPTWKRDKSVDPNIWLDQQTDLFNEVSWAVMYIFNTNEKYSKFFLDNYETYKA
                     KYKFEAQDEINNIIYGFMNTAVKENKPELMTSAESLMQKYFPEDTSGLFYLRLNYMMS
                     VGDNNGLANLMSNYIATNKEVYLGTVNAAAWNLYENCDDQKILQTAISWFQPYLPTIT
                     DYFAMDTYAALLYKTKQYASAEEWAIKAIALGKADGEDMAGTEELLEKIKKEK"
     gene            3339..4061
                     /locus_tag="PLL28_09345"
     CDS             3339..4061
                     /locus_tag="PLL28_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69567.1"
                     /translation="METGQRKFGKTLLPTKNYMQVALIEIMLIFSVLLMGDIAAALGS
                     SSTGSAVSMALLIPEAVYVYILFRLTTLLTDNRVMQRIVAVLLVVAAILSVVGLLPLM
                     SGDESKSQVLIAVHLILCFIECYLIALGLQDIYIDKLTLRERLWGSVAMYLLIAIGWA
                     SFYEIFLLTDHTLFGVELIPGYQTYSEALYYSLCSLSGTGSIYANPNHLIRNLSLIES
                     VWGVLFLVMLIGRVFTVSDKEE"
     gene            4143..5318
                     /locus_tag="PLL28_09350"
     CDS             4143..5318
                     /locus_tag="PLL28_09350"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69568.1"
                     /translation="MKKLLLLLLLGVYTGIFAQSGSMEFTSSGTFTVPDGVTTIFIEV
                     VGAGGSGGYNGTGGGGGGGYASGNYSVTPGQVIAIAVGSPGVSPMVGTTSVEGFLSAS
                     GGENGVSVPNPEIGGGGDGGIGAGGNIVNHTGGNGGGGYYTYFGGGGGGAAGAVGDGA
                     DGGNTIAWTGICLTPGGDGGISGGAPGGNGGKGAGFTDAFCNVTDPAAVGANYGGGGG
                     GGNGNGGAPANGAGGYCFISWCNINVGIVANEDVIAAEETDATFQWINCDDNSIIEGE
                     TGNAFIPEVSGNYAVIVNDGICADTSECVAFTVIEQSISDVIGQQITISPNPVHDVLH
                     IIAPAGMDFTYALFSTTGQCLWISKDVQHPVSHLNNGIYLLRISNENAYAWKLFVKE"
     gene            5502..7799
                     /locus_tag="PLL28_09355"
     CDS             5502..7799
                     /locus_tag="PLL28_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNF69569.1"
                     /translation="MSSFLDELNEAQRAAVTNVSGPTMIIAGPGSGKTRVLTYRVAYL
                     METGIDPFSILALTFTNKAAREMKNRIEKVMGGDARSLWMGTFHSVFSRILRSEADKL
                     GYPQNFTIYDTDDAKNLLKNIVKEMGLNDKLYKPGYCLYRISLCKNNLIGPAEYANNT
                     DFIAEDEANGRPKMSEVYQQYAKRCFRAGAMDFDDLLLKTHELFERFPDSLYKYQHRF
                     KYVMIDEFQDTNHLQYAIVRRLADVYQNIAVVGDDAQSIYAFRGANIANILNFEKDFP
                     EVQTFRLEQNYRSTKVIVEAANQIISKNRHQLAKTIWTDNTEGDTIKVIRAMSDNEEG
                     RLVADQIFEQRMRDHFKNGEFAILYRTNAQSRSFEEALRRLNIPYRIYGGLSFYQRKE
                     IKDLVAYLKLTVNPNDEESFRRVINYPARGIGKTSFEKLTVFAADNEKSLFTMAENID
                     LLPFNAGTRKLFTDFITMIRSFQTMLETKDAYEVASHIAKSTGILKELYNDQSVEGLS
                     RYENVQELLNSIKEFASPDEANSPYFTETLPTENTGPRSLAEYLQQVVLLTDADQEGN
                     EDETVKLMTIHAAKGLEFKSVFVVGLEENLFPSALSVNSRDELEEERRLFYVAVTRAE
                     KKLVLTHAASRYKYGNLEYCEPSRFLEEIPQNLLTYVGYNKTSRQDERNIFDKAYEHA
                     TPIAKAKPVAPVQNYVHAPSAGFKVAFPEELKAGMEVEHQKFGFGTISALEGQNDGLM
                     ATINFHKAGQKKLLLKFAKLAIVEK"
     gene            complement(7813..8271)
                     /locus_tag="PLL28_09360"
     CDS             complement(7813..8271)
                     /locus_tag="PLL28_09360"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HNF69570.1"
                     /translation="MEVFRLAREKYAHALSGKGSAIKGARWNSVGVELIYTASNRSLA
                     MAEVAVHLTLATLPGDFRMFTIHIPDTIAIKEVKMKYLPTNWQEFPHPATTQRFGDEF
                     VAANKYCVLKIPSVVTQGDFNYLINPYHADFKKIKIIEIVNFPFDKRIVK"
     gene            complement(8275..8778)
                     /locus_tag="PLL28_09365"
     CDS             complement(8275..8778)
                     /locus_tag="PLL28_09365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HNF69571.1"
                     /translation="MAAVKQTLEKKLDKEIGIYFQTTVTKEFKFDDKKRITYEDFLSD
                     RMLIIAAIRVGIPYSLFELIQIYTPFSENDWADLLDVSTKSLQRYRQASQHYFKSFHS
                     EKIIEMAEVTKLGLEVFEDMDKLKLWLNTPNFALGKIKPIELLKDSYGKDLVTSELVR
                     INHGILV"
     gene            complement(8876..9631)
                     /gene="truA"
                     /locus_tag="PLL28_09370"
     CDS             complement(8876..9631)
                     /gene="truA"
                     /locus_tag="PLL28_09370"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNF69572.1"
                     /translation="MRYFAEIAYRGTHYVGWQIQPNGVSVQQKVNEALRKILRDEEIA
                     CVGCGRTDAGVHATQFFLHYDTYAEIPETFLLRMNTVLPDDIVVKSHFPVAENAHARF
                     DATLRAYEYFISFNKDAFGPDRVLWRHFRDLDFDLLQACCEVMKKYNDFPSFCKSKQG
                     SKTTIINLQEIYWRQEGDTYILFISADRFIRGMVRLVVGAMMQVARGKMTIEEFESGI
                     QKGERLKLALSAPAHGLYLMRVEYPYQFGTLPE"
     gene            9942..11150
                     /locus_tag="PLL28_09375"
     CDS             9942..11150
                     /locus_tag="PLL28_09375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNF69573.1"
                     /translation="MKIATTPSRLAAYSTMAATVLAIQNEADGQVIYTNINPDKVLQS
                     TGPYDTLHLDVTGDAITDFIFNAFYFYTSYTASAVNYDHLGVDGINGARIAYTMQSTN
                     IYTWSSALFANLSAKFAQILPANTPINNTLNFNNNVELFYQQCFPAMYSSMMDYCWTA
                     GMPSAVVKYIGFRLKNGPLKYYGWMRLSIHKSTPFVPVPTSIKIYDYAINWTPNTPIL
                     AGEGLICIATDAVGTGTTASHWAKVYWEPRVDIDHYEVSYRIPGGAWETKTVPPDKTS
                     TKLNGLECSTDYEWQVRCACLDGSLSDYSEVQTFTTASCRLEGEDMTENDEAVSMYAN
                     ANRIYIYFDEVVTEPSRLFIYNMTGQLVSETIVTQQQNILEVSVPSGMYTAKLVTPSG
                     NIGKLISISN"
     gene            11258..11635
                     /locus_tag="PLL28_09380"
     CDS             11258..11635
                     /locus_tag="PLL28_09380"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 family protein"
                     /protein_id="HNF69574.1"
                     /translation="MWQRIQSIFLALAAIAAIAFLFVDIADGLTGKDNVAASAVAVGI
                     ALLQGFIIAMYKDRRVQMRFCATTMLLAAVMAALAYMKTDGENLRLAFIFGIPAVIMI
                     TSFLARYNIKKDEDLVKSMDRMR"
BASE COUNT         3490 a         2345 c         2689 g         3616 t
ORIGIN      
        1 tttctaaggt ctcctgttca gaggtgttta ttcccggacg tgtttttgtc ctcggtttcg
       61 gcggcgattt gcttttgcgc ttggaactca tttttgtcat taaagcatga tccttcacat
      121 gttgcctgat cacttcaata aactgcggtc cgtatttgcc gattttcact tcaccaaatc
      181 cgctgatatc ttttaaatct tcaatttgca ccggtaagta agccgccatt tcctgcaaag
      241 tgctatcgcc catgatcata tagggcggta aactttcgcg ataggcaatg cgtgttcgca
      301 gactgcgcag gaggtcaaac agatggctgt catattctat ctgattgctg ttttctacca
      361 tcaaacgttc ttccgacaag gtgaaatata cctgctcggt gccttgtaga acacgcttgc
      421 ctttttcagt gatgagcaat cgcgggtaac tgccttctgc aatgcgtttt acatattgct
      481 gatcgattaa ctcttgaaaa tactgcatcc atttatcgcg tgaaatatct gcaccaatgc
      541 cataggtttt atattgttta tgacctttcc atattttttc actgcgactt ccacgcagaa
      601 agtctacgag ataatgtatt ccaaatcgct cttcggtgcg cacaatggca ctcaatgctt
      661 tttgtgcaat ggtggtagct tcgaaagatg ttttacgact taagcataca tcacatgatc
      721 cgcaatttga ttcgggatat ttttctccga aataatcgag tagcattgtg cgtcgacaaa
      781 tattcgatac gcaaaatgca ttcatgcgca ttaatttatc cagcatgatg cgggtgcgtt
      841 caggattgcc ttctacttcg gcaaatcttc gcaattgcat caaatcgcca taactataaa
      901 atagaatggc atcactaggc aaaccatctc tgccggcgcg tccggtttcc tgataatatg
      961 attcgatatt tttcgggagg ttgtaatgta tgacatagcg cacatccggt ttatcaattc
     1021 ccataccgaa tgcaatagtg gcaacgatga tatttgtttt atccttttta aatgcctcct
     1081 gaacagtatt gcgcgatgcg gcatccattc cggcatggta atatgctgct tcaaatccat
     1141 ctgccgtcaa tcgctcggca agcatttcgg cattggcgcg acttaatgta taaattatgc
     1201 cgctttgccc ttgatgttgc acgatgtaat taatgatagc agtataggca tcgcgttttt
     1261 gttcaatgta ataattgatg tttgcgcgat taaaactgga tattgaaata tgtgctttcg
     1321 gcatatttaa tttatccaga atatccgccc tcgtctgggc atcggcactg gctgtaagag
     1381 cgataattgg cacccgcgta agcttttctc gaattttagc gagttttaaa tattccggtc
     1441 tgaagtcgtg tccccacatg cttacacagt gcgcttcatc aatggctatg aggctgattt
     1501 tcagggtaga taagagctct atcaacctac cttcatcacc cattaatcgc tcaggtgcta
     1561 tgtataataa tttgatttcg ccacgcaata ttttttgcgt tactatgcgc tgttcatcgg
     1621 gtgttaaact actgtttaag gcaactgcat taattccgtt tgtcaccagt gcatccacct
     1681 gatctttcat gagtgcaatc agcggggaaa ccactattgc catgccttcc agcatcatgg
     1741 ccggtatttg ataacagacc gattttcctc cgccggtagg cataacaacc agtgtgtcat
     1801 gaccacttgt aatgtgatta attacctcct cctgctgatc gcgaaacgca tcatatccga
     1861 agtagcgttt cagcactttt gccgcatcca tcgattccat aacttgcaaa tgtaggacga
     1921 ttcatcatac tagtccgctt ataagcgttt atatgcgaat aaaagcggtt atgataggtt
     1981 aatgatcttg tttttcgcgg aaattaattg tcatttgatt gtctgcccgc ccgaaacgct
     2041 tgcttttgtt acataggttt gatttacttt gtgtcgttaa atatttattg aattatgaaa
     2101 agaatactga cagttctaat cgcactcctc ccgctgatca attttgcgca ggacgtgcaa
     2161 tttttccaag gcacctggga agaagtaaat gccaaagcga aggcagagaa caaactcatt
     2221 tttgtagacg cctataccta ttggtgcggc ccttgtaaaa tgatggatga aaccatgttc
     2281 cacaataata aagaagttgc tgattatgtc aatgcacact ttattgcata caaggtggaa
     2341 tgtgagcatg attttggcgt tgaatttgcg cggaaatata aagtcaatgt atacccaacc
     2401 atgttaatgt ttaacagctc aggtcaatta cttgaaaaac atttaggata taatgatgtt
     2461 caatctgaat ttatagagac cttcaggaaa gtggtgaata tggatcaaac agatgtttat
     2521 gcaatggatc ctgcaaaaat aaaaatgaac tggcccgaat tttatgccaa gaattttaaa
     2581 gatgccaatg accctacctg gaaacgcgat aaatctgtag atccaaatat ttggctcgat
     2641 caacagactg atttgtttaa tgaagtgtcg tgggcagtga tgtatatttt taatacgaat
     2701 gagaaatact caaaattctt cctcgataat tatgaaacct acaaggcaaa atataaattc
     2761 gaagcgcagg atgaaatcaa taatattatt tatgggttta tgaatactgc agttaaagaa
     2821 aataagcctg aattaatgac cagtgctgaa agtttaatgc aaaaatattt tcctgaagac
     2881 acttccggat tattttactt gcgattaaat tacatgatgt cagtcggcga taataatggt
     2941 ttggctaatc ttatgtcgaa ttacattgct acgaataaag aagtatattt aggcaccgta
     3001 aatgctgcgg catggaattt atatgaaaac tgcgatgatc aaaaaattct tcaaacagcc
     3061 atttcatggt ttcagcccta tctgccaact attaccgact attttgccat ggatacctat
     3121 gcagccttgt tgtataaaac aaagcaatat gcttcggctg aagaatgggc gataaaggct
     3181 attgccctag gtaaagctga tggtgaggat atggccggaa cggaagaact cctcgaaaaa
     3241 ataaaaaagg aaaaatagcg ctgtccgaat ttctttatat aagagtcgat cttttccggg
     3301 tcggctcttt ttgttaatat ttagtgtaat tttatcttat ggaaacaggg caacgcaaat
     3361 tcggaaagac tttattgccg acaaaaaact atatgcaggt agcacttatt gaaatcatgc
     3421 tgatattttc agtattatta atgggtgata tcgctgcggc gcttgggagc agctctaccg
     3481 gttcggcggt ttctatggcc ttgttaatcc cggaggctgt ttatgtatat atacttttta
     3541 gattgacaac cttattgacc gataatcgtg ttatgcagcg tatcgttgct gtgttattag
     3601 tggtagcagc aatattgtcg gtggttggct tgcttcctct gatgtcggga gatgaaagta
     3661 aaagccaggt gttgattgct gtacacctga tattatgttt tattgaatgt tatctgatag
     3721 cacttggatt gcaggatatt tatatagata aacttaccct gcgagaacgt ttatggggaa
     3781 gtgtggcaat gtatcttttg attgcgatag ggtgggcgag tttttatgaa atatttttgt
     3841 tgacagatca taccttattt ggtgttgaat taattcccgg ttatcaaaca tattctgaag
     3901 ccttgtatta cagcctctgc agtttaagcg gaacgggaag catatatgca aaccccaacc
     3961 acctcatacg caacctctca ctaatagaat ctgtttgggg tgtattattt cttgtaatgt
     4021 tgataggacg cgtatttaca gtgtcggata aagaggagta atgtgttaaa tgttttcctt
     4081 gctttcatcc ggcaatcatc acatcatttg tttgattata tttatctcct gatcttcaaa
     4141 caatgaaaaa attactactc ctccttttgc ttggtgtata caccggcata ttcgctcaat
     4201 ccggcagcat ggaatttaca tcttccggaa catttacggt tcccgatggc gtaacaacaa
     4261 tatttattga agttgttggc gcgggcggaa gcggtggata caatggaacc ggcggcggtg
     4321 gcggaggtgg ttatgcaagc ggaaattatt ccgttacacc cggacaagta attgcaatcg
     4381 ctgtcggttc acccggtgtg agtccgatgg ttggaactac cagtgttgaa ggttttctga
     4441 gtgcttcagg cggagaaaat ggcgtatcgg tacctaatcc tgaaattggt ggtggcggtg
     4501 atggtggcat tggtgcagga ggaaatattg tcaatcatac cggcggaaac gggggtggcg
     4561 gttactatac ttatttcggt ggcggaggag gtggagccgc aggtgctgtt ggtgatggcg
     4621 ctgatggtgg taataccatt gcatggacag gtatttgtct tacacccggt ggtgatggcg
     4681 gaatcagtgg tggtgctccc ggcggaaacg gaggaaaagg cgccggattt acagatgcat
     4741 tttgcaatgt aactgatcct gctgctgtcg gcgcgaatta tggcggtggc ggtggtggtg
     4801 gaaacggcaa tggcggagct ccggcaaacg gtgcgggtgg atattgtttt atttcctggt
     4861 gtaatatcaa tgtaggaatt gttgcgaatg aagatgtcat tgcagcagaa gaaactgatg
     4921 ctacgtttca atggatcaat tgcgacgaca atagtattat tgaaggtgaa acgggaaatg
     4981 catttattcc ggaggtaagc ggtaattatg ctgttattgt gaatgatggt atttgtgctg
     5041 atacttcaga atgtgttgca tttaccgtta ttgagcaaag catttcagat gtaatcgggc
     5101 aacaaattac tatttcacca aaccccgtgc atgatgtgct gcatattatt gctcctgcgg
     5161 gaatggattt cacctatgca ctgttctcaa caacaggtca atgtctctgg attagcaaag
     5221 atgtacagca tccggtatca catttaaaca acggaatcta tctcctccgc atatcaaatg
     5281 aaaatgctta tgcatggaaa ttgtttgtga aggaatgaga gagggtcagt gggtgagtgg
     5341 gtgagtatta gggggggatt gtatgagaaa aaaatattga gcgtgccatt ttttcgcgca
     5401 aagacagcta agaggctaag gcgcaaagtc ggtcatgctc aaaaaactca ccactcacca
     5461 ttcaccactc accattcccc tcctttcctt aaatttgctc cgtgagtagt tttttggatg
     5521 aattgaatga ggcgcagcgc gcagccgtta ccaatgtgag cggaccaaca atgatcattg
     5581 cagggccggg gtcagggaaa actcgtgtgc ttacttatcg tgttgcttat ttaatggaaa
     5641 cagggataga ccctttttct attcttgcgt tgacatttac caacaaggca gcacgagaaa
     5701 tgaaaaaccg catcgaaaaa gtgatgggcg gtgatgcgcg gagtttgtgg atggggacat
     5761 ttcactcggt attttctcgt atcctgcgta gtgaggcaga taaactcgga tacccgcaga
     5821 atttcaccat ttatgatacc gatgatgcaa aaaatctgct gaagaatatt gtgaaggaaa
     5881 tgggattgaa cgataaactg tataaaccgg gttattgttt gtatcgcatt tcgctatgta
     5941 agaataatct cattggtccg gcagaatacg caaataatac tgattttatc gcagaggatg
     6001 aagcaaacgg tcgacctaaa atgtcggagg tgtatcaaca gtatgccaag cgatgtttca
     6061 gagccggggc gatggacttt gatgatctgt tgttgaagac acatgaatta ttcgagcggt
     6121 ttcccgactc attatataaa tatcagcatc gctttaaata tgtgatgata gatgagtttc
     6181 aggataccaa tcatttgcaa tatgctattg tgcgccgact ggcagatgtg tatcagaata
     6241 ttgccgtagt gggtgatgat gcacagagta tttatgcctt ccgcggagcg aatattgcca
     6301 atatcctcaa ttttgagaaa gattttcctg aggtgcaaac attccgactg gaacaaaatt
     6361 atcgcagcac gaaagtgatt gtggaagcag caaatcagat catcagtaaa aacagacacc
     6421 agctcgcaaa aaccatttgg acagataata cggaaggcga taccatcaag gtaattcgcg
     6481 cgatgagtga taatgaagaa ggaagacttg ttgccgatca gattttcgaa caacgcatgc
     6541 gcgatcattt taaaaatggt gaatttgcca ttttgtatcg caccaatgcg caaagtcgct
     6601 cgtttgaaga ggcacttcgc agattgaata ttccgtatcg tatttatggt ggattgtcgt
     6661 tctatcagcg caaggaaata aaggatctgg ttgcatattt gaagctgaca gtaaatccga
     6721 atgatgaaga atcgtttaga agggtgatca attatcccgc tcgcggtatc ggaaaaacat
     6781 catttgaaaa attaactgtt ttcgcggcag ataatgagaa gagcttgttt accatggcag
     6841 agaatatcga tctattgcca tttaatgccg gcacacggaa attgtttacc gattttatta
     6901 ccatgatcag gagttttcaa accatgttgg aaacgaaaga tgcttatgaa gtagccagtc
     6961 atattgcaaa atctacaggc atactgaaag agctctataa cgatcagagt gtagaaggac
     7021 tgagcagata cgaaaacgtg caggagttgt tgaactccat caaagaattt gcctcacctg
     7081 atgaagcaaa ttctccttat ttcacagaaa cattaccaac ggaaaataca ggtcctcgca
     7141 gcttagcaga atatttgcaa caagtagttt tgctcaccga tgccgatcag gaagggaatg
     7201 aagatgaaac cgtgaaactg atgaccatac acgcggcaaa aggattggag tttaagagtg
     7261 tatttgttgt tgggttggaa gagaatttat ttccaagtgc attgtctgta aactctcgcg
     7321 atgagctgga agaagaacga cgattatttt atgtagcggt cacaagggca gaaaagaaac
     7381 tggtgttgac acatgctgct tcgcgatata aatatggcaa tctggaatat tgcgaaccga
     7441 gtagatttct ggaagagata ccacaaaatc tgctcacata tgttggttac aataagacat
     7501 cgcggcagga tgaaagaaat attttcgaca aggcatatga gcatgccaca ccgatagcga
     7561 aagccaagcc tgtagccccg gttcaaaact atgtgcatgc accttcagca ggattcaaag
     7621 tggcttttcc ggaagaatta aaagccggca tggaagtgga gcatcagaaa ttcggatttg
     7681 gaacaatctc agctttagaa ggtcagaatg atggcttaat ggcgacaatt aatttccata
     7741 aagcggggca gaagaaattg ctgttgaagt ttgcgaagtt ggcgattgta gaaaagtaat
     7801 tgcagtaaaa tcctatttaa ctattctttt atcgaatggg aaatttacta tttcaatgat
     7861 ttttattttc ttgaaatctg catggtaagg gttgatgagg taattaaaat ctccttgtgt
     7921 cactaccgaa ggtattttta acacacaata tttattagcc gctacgaact catcaccgaa
     7981 acgttgtgta gtggcaggat gtggaaattc ctgccagttg gttggaagat atttcatttt
     8041 gacttcctta atggcgatag tgtcagggat atgaatagta aacatcctaa aatcgccggg
     8101 caatgtagcg agtgtgaggt gcacggccac ttcagccatt gccaacgacc tgttcgaggc
     8161 ggtataaatt agttcaacac cgactgaatt ccatcgcgca cctttaatag ctgatccctt
     8221 tccggagagc gcatgagcat atttctctcg tgctaatcta aatacctcca caacttacac
     8281 cagaattccg tgattaattc ttaccaattc cgaggtaact aaatctttgc cgtaagagtc
     8341 ttttaataac tcgatgggtt tgatttttcc caacgcaaaa tttggagtgt taagccatag
     8401 ctttaatttg tccatatctt cgaaaacttc cagacctaat ttagtgacct ctgccatttc
     8461 aataattttt tcagaatgaa atgacttaaa ataatgttgt gaggcttggc gatatcgctg
     8521 taaagatttt gtcgaaacat ccagaagatc agcccaatcg ttttctgaaa aaggggtgta
     8581 gatttgaatt aattcaaata gtgaataggg tattcctact ctgattgcgg ctataatcaa
     8641 catcctgtct gaaagaaaat cctcgtatgt gatcctcttt ttgtcatcga atttgaactc
     8701 tttagtcacg gtagtttgaa agtaaatacc gatttcttta tcaagttttt tttccaaagt
     8761 ttgctttacg gcagccatgg tatatagaca tttgtctgta aaaataggac aaatgtctta
     8821 caatcccaaa tttatttggc caagggctag tgttcaggcc tttatggaac tattttcact
     8881 caggcaatgt gccgaattgg tatggatact ccaccctcat caaatacaat ccgtgagccg
     8941 gagcgcttaa agctagcttc aggcgttcac ctttttggat acccgattca aattcttcaa
     9001 ttgtcatttt tccgcgggct acctgcatca tcgcccctac aaccaatcgt accataccgc
     9061 ggataaatct gtcggcactg atgaataaaa tgtatgtatc accttcttgt cgccaataga
     9121 tttcctgcag attgataata gttgttttcg agccctgttt gcttttgcag aaagaaggga
     9181 agtcgttata ctttttcatc acctcgcaac aagcctgcaa caggtcgaaa tcgaggtcgc
     9241 gaaaatgccg ccataagact ctgtcgggac caaaggcatc tttattgaag gagatgaaat
     9301 attcataagc tctaagggtt gcatcgaagc gggcatgagc attttcggca acagggaaat
     9361 ggctttttac cacaatatcg tcggggagca cggtattcat acgcagcaga aaagtttcgg
     9421 gtatttcggc atacgtatca taatgcagaa aaaactgagt agcgtgaacg ccggcatctg
     9481 tgcgaccgca gccaacacag gcaatttctt catcgcgcag aatttttctg agggcttcat
     9541 tgaccttctg ctgcacgctg acgccattcg gctgtatctg ccagccaaca taatgtgtac
     9601 cgcgataagc tatttctgca aagtagcgca tgtggcaaag gtaggagaaa tgtgtgtgat
     9661 ggtcatttat atgaccttaa acgagctgct gaatatctcc tcttgtttct tcttgccggt
     9721 gagttgcaat ctgagctgta tttcttagtc ggggaatcaa gtttaaatag gcgttttatt
     9781 ggaattaacc ctcctataga cataaaattg acgctaattc agtccaaata gcatataaat
     9841 ttatctaatg ttatcccatt ttatggccat tgtgcgcgtt tcactatttt atgtaagtgg
     9901 aagttgagtt ttattatatt aaatttgcaa taaaactcaa tatgaaaatt gcaactaccc
     9961 cttcgcgact tgcagcttat tctacaatgg ctgcaactgt attagccatt cagaatgaag
    10021 ctgatggtca ggtcatctac acgaatatca atcccgacaa agttttgcaa agtacaggtc
    10081 cgtatgacac gctgcatctt gatgttaccg gtgatgcaat tacggatttc atttttaatg
    10141 ccttttattt ttatacatca tatacagcat ctgctgtaaa ttacgatcat ctgggggtag
    10201 atggtattaa tggtgccaga attgcataca ccatgcaatc gactaatatt tatacatggt
    10261 caagtgcatt atttgcaaat cttagtgcaa aatttgcgca aattttacct gcaaatacac
    10321 ctattaacaa taccttgaat ttcaacaata atgttgaatt attttatcag cagtgcttcc
    10381 ctgccatgta ttctagcatg atggattatt gttggacagc gggaatgcca tcagcagtgg
    10441 ttaaatacat tggtttcaga ttaaaaaatg gtccgttgaa atattatggc tggatgcggc
    10501 tttcaattca taaaagcacg ccgtttgttc cggtaccaac ctccattaag atttatgact
    10561 atgccatcaa ctggacgcca aatacgccga tattggcggg tgaaggatta atttgtattg
    10621 ctacagatgc agtaggaacc ggcacaactg cttcacattg ggctaaagta tattgggagc
    10681 cacgtgttga tatagatcat tacgaagtgt cttaccggat acccggaggg gcatgggaaa
    10741 ctaaaacagt tccgcctgat aaaacaagta ctaaactaaa tggattggaa tgtagtacag
    10801 attatgaatg gcaggtgcgt tgcgcttgtt tggatggttc gttgtccgac tattcagagg
    10861 tgcaaacttt cacaacggca tcatgcaggt tagagggaga agacatgaca gagaatgatg
    10921 aagctgtttc aatgtatgct aatgcaaatc gtatatatat ttatttcgat gaagtagtga
    10981 cagagccatc tcgtttattt atttataaca tgacaggtca attggtatcg gaaacaattg
    11041 taacgcagca gcaaaatata ttagaggtat ctgttccttc aggaatgtat acggccaagc
    11101 tcgttacgcc aagtggaaat attggcaaat tgatttcaat ttcgaattga gtatctgtca
    11161 tctcaatttt tatgagtatg accgggcagt gtaggcgcct gtagtggaaa taatagaatg
    11221 atatctgatg cctatctttg caggaaatta gtttactatg tggcaacgca ttcaatctat
    11281 atttctggca ctggcagcaa tagccgcaat tgcttttttg tttgttgata tagcagatgg
    11341 cttaacagga aaggataatg tagccgcatc agcagtagct gttggaattg cgttattgca
    11401 gggatttatc attgccatgt ataaagaccg ccgggtgcag atgcgttttt gtgctaccac
    11461 catgttgctc gcggcagtca tggcagccct ggcctatatg aaaaccgatg gagaaaacct
    11521 ccgactagca tttatcttcg gcattcccgc tgtcattatg atcacctcct tcctcgctcg
    11581 ctacaacatc aaaaaagatg aagatctagt gaagtcgatg gacaggatgc gttgatatat
    11641 atttccgaac cggacaccta aagccacatt aaacaaaagc atttccggga aaattgccgg
    11701 attgtcctga tcaaagcaca gaccggcaaa taaaaccact gcgacccacg tgggcgagct
    11761 gctacaaagg cggcaaacat acttacaggc tattccccaa aatgtgacac cacaaatgca
    11821 ccaatatttg tccaaaatgc agctgaaaac catttctgtc gggcatttac accttaaatt
    11881 tcatccattt ttgtgacata ttcacattca cggttttccg ttaaccttca atccgtccgg
    11941 aagtgtaaat ttgtagatag caaattgctt gtaaattaac ttttgtttcg ctttagcttc
    12001 cggtttgggg tgttaggcga aatggggttg cggcaataaa caagttatcg gcaaccctat
    12061 ttgacgacac agcaaccttg gaacaaacag taaaacgaca gacaaaatgc caacgcttcg
    12121 caaaataaaa agagctgcaa
//
LOCUS       DAOQHY010000103        11890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2642_length_11890_cov_5.858386, whole genome shotgun sequence.
ACCESSION   DAOQHY010000103 DAOQHY010000000
VERSION     DAOQHY010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11890
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2642_length_11890_cov_5.858386"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1475)
                     /locus_tag="PLL28_09385"
     CDS             complement(<1..1475)
                     /locus_tag="PLL28_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69575.1"
                     /translation="MMKRNAPGWILALVTAVIICAAWQKIIFSPNHYLLTTGGDAFKN
                     YYTPAWYVANDKGDRFTGMNYPYGEHVVFTDDQPMISWLINVIDDHVFPVHDNTVAIL
                     HLLMFISLWAGVLLIFHILRHYRMPAWYAAGMALLIGLMTPQLDRFWGHFALSYALFV
                     PLIWWLMIRLEKGNYQIRRALLLVAVVIFFGFIHMYYALIASLFIGFYALTILCRSFR
                     KWRLSLYIMALAAIPVVAILGFMHVTDHVHDRPETPYGFFRYKASFQSVFLPKHGTLT
                     TQLQKHLHVGHANPEGYAYVGYAGLLFLFVLLVIFTKRILYKRNIRRSLIMLPEGLSI
                     YVWTGIAMLLFSMSLPFSLGLQSLLDILTPLKQFRSPGRFAWGFYYVYMVTIAVLSWM
                     IFKKIKKRQRILSYVFIFIFCGILTADSLTYFFQTSRDLQKANAENPFDGKTTYFSDA
                     LLGTNYTEKDFDAILFLPSFFQGSEKLYIDRTDGHLSKAME"
     gene            complement(1472..2197)
                     /locus_tag="PLL28_09390"
     CDS             complement(1472..2197)
                     /locus_tag="PLL28_09390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF69576.1"
                     /translation="MSQPTLDIILPCYNPAPSWVDHIITGYGEIAAVIPHADIRVILI
                     NDGSSRSIGDEDLLQLKSAIAKFECINYQKNRGKGYALRQGVAASNADITIYTDVDFP
                     YTTASFIQLYTVLANKEADVAAGVKDATYYAHVPAFRRFISKLLRACTTTVLRLKVSD
                     TQCGLKGFNDSGRDMFMRTSIDRYLFDLEFIFLASRNKSLKIKPVEIALRDNVQFSSM
                     RMSILLQESGNFLKILFRSIFTR"
     gene            complement(2324..3265)
                     /locus_tag="PLL28_09395"
     CDS             complement(2324..3265)
                     /locus_tag="PLL28_09395"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNF69577.1"
                     /translation="MKKVHYIILGAVIGVGLIVALAVKGNANGNGAHVESAMPTDTLR
                     SLADEVNTIRYFAAKLPKTLTFAGEEVPLDDPEVRERLDRELTVTGYWHSNTIQNLKL
                     ANRWFPIIESTLKKNGVPEDFKYLAMAESGLRNVISPSGAHGYWQFLENTGEHYGLTI
                     NGEVDERYDMEKSCEAASQYFKDAYSKFNNWTLAAASYNAGMGAIEGAVNYQDEKSYY
                     DLYFREETSRYVFRALAFKIIYEHTQDYGFFLTKEDLYDPLQYKEVTVTTSIPNLSEF
                     AKKNGTNYKMLKYYNPWLRSDMLTVKAGQQYRIKIPA"
     gene            complement(3262..3774)
                     /locus_tag="PLL28_09400"
     CDS             complement(3262..3774)
                     /locus_tag="PLL28_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HNF69578.1"
                     /translation="MELVNKVAQSGLLTFDLEDLFPAEGSIVSFDLKDYLFRGLILRE
                     KDFREALAAIDWQQYAGKTVAVYCSADAIIPNWAWMLVAANVAGIAEMVVMGDPELAM
                     KRYYTQQLSQVDLEQYRNQRVVIKGCSDKPVPAEAYLEITALLRPVTKSIMYGEPCST
                     VPVFKKKEQA"
     gene            complement(3915..6098)
                     /locus_tag="PLL28_09405"
     CDS             complement(3915..6098)
                     /locus_tag="PLL28_09405"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNF69579.1"
                     /translation="MLISKIRQYSWLAVGLIALCLIGFLVQDATNSNTGMFRGNKAPD
                     YATMYGEEVNRDEVAERSGRTLLEYLTLQTGQVLQYENGSMQLDPQTKFGLNEQSWTE
                     YVNEKIIDKQLEELGLGVTEEELTNLIYGPDPHPYIKNYYIGLSQTGTYDASLLPLHV
                     KAISDPETQKNDPSARQAYYNFICREQIAKRDYRQTKYMSLFSKAAYVPEWMAKRNYT
                     VGNTRRAFRMVSMPYAQIADSTIVVNDKDLETYYNKNKNKYKQTEGRTVEYVSWDFIP
                     SPADSAATVAALMESVAKMKTAKSDSAFIATRSEDPDRFGNSNYSRQDLYTQGIDSAY
                     VDSFFVRPAGSLIGPFQTGEYYKVATLKSRTMMPDSVKSRHILVGISEQRDSIAAKKI
                     VDSLMGLLNAGGDFTKIAGESSDDQSSAQQGGDLGWTTPSINFVPEIKDYLFKTGSLG
                     KVSVVKSMYGYHIIEITEMHNKKEFVNVAFLSKSIGPGKETVSSIEKMADDFYAAHQD
                     PESFEQGVIDEKLFKRTTQPLTKSMFEVPGLPNSREIITWAYESDLNEFKYFNMTDRV
                     VVAYLKEIRVNGVADLEYVRDQVEQEVILEKKAEILTKKINEAMGSGATMESIASKLG
                     VRVDSVLNASMATPSAPILGREPKVIGAVFAAEQGKITAPISGLRGVYVAQVFQETPA
                     PEQTDFTMNKNQLTYSMQNKFQGQGLLIVLKERANVVDNRYIYGD"
     gene            complement(6240..6536)
                     /locus_tag="PLL28_09410"
     CDS             complement(6240..6536)
                     /locus_tag="PLL28_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69580.1"
                     /translation="MAKKEAPNMYVRLMKIFEVMWLVTAAVALVMAITRALNGQQYGN
                     YIYITLFTGSVAYFMFRFKKRNRLYLEEHYRKQEEMAKKQAGASKSGETEAPKA"
     gene            6611..7351
                     /locus_tag="PLL28_09415"
     CDS             6611..7351
                     /locus_tag="PLL28_09415"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNF69581.1"
                     /translation="MQLVLDLGNTKHKYAVYEGNELVRLGSEPQLTAEITANLIDIYG
                     IDQAIVSSVDHFDEALESLLEERTGLLQFTHTTPLPIINLYGTPATLGKDRLAGAVGA
                     HTAFKGSPVLVVDAGTCIKYDFLDKEGIYYGGAISPGLFMRFKAMHNFTARLPLIDPE
                     NYATVLEVPLTGTSTVDSLRSGALNGALFEVEGAISAYTGQHAGLAVILTGGDAGFFE
                     LHMKSRIFARPNLVLEGLHTILNYNLNH"
     gene            7351..8745
                     /locus_tag="PLL28_09420"
     CDS             7351..8745
                     /locus_tag="PLL28_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69582.1"
                     /translation="MHLRILPLLALTTLTLSISAQVNSPYSRYGLGNMYPGSFTAANG
                     MGGFSAANFSPSAINFLNPASFAEIGGASFDVGVYGTVMSLNTNSETFTSGDANLSNM
                     AFAFPVYKNLRRDRFGLSFGLVPFSTFQYDILDETDTGDPDLGTKQYNYVGDGQLYQL
                     YGGFGYRHQTDTVSQVKSIQGRQDTVISAGFLSIGASSALLFGSLYNTTYASFPDILN
                     SQTTKLSRDNDVNGGMYNIGVGYQKQYVRKHGTKRDILNWKFGGTFSPQVDVHGTQSI
                     LWTNILKYGNYETVTDTLYTAPDTSGNIHMPMSYSAGLSFSFFSSDDENKNQFTLGLQ
                     YASTMWSQYDGFQDAGELADSWRATLGAEFIPKKKQDQNAPLMYRFGAYTGKSNLIID
                     GVQLNDVGFSAGLAIPMGYGENVLPYYRNSKINMFLNVGQRGANADISETYFNFGVGF
                     SMIDLGWFMKYKLN"
     gene            8769..9326
                     /gene="lptC"
                     /locus_tag="PLL28_09425"
     CDS             8769..9326
                     /gene="lptC"
                     /locus_tag="PLL28_09425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNF69583.1"
                     /translation="MITSTWMSKYPLTLLVAMTIFAGCVNNVNEVNKATQLAEPGIEY
                     GKDIELFFSEDGEVKIRVTAPTVVRHNTEDPYTEFTDGLKVEFYNDSMHVDTWLTANS
                     GTRYEAQGRTVLQNDVQVLNKKNEHLSTEELIWDEKRHIIYSDKFVKITTPDQVLYGD
                     GMEADEQLTRYTIKKPRGTIQAEME"
     gene            complement(9412..9726)
                     /locus_tag="PLL28_09430"
     CDS             complement(9412..9726)
                     /locus_tag="PLL28_09430"
                     /inference="COORDINATES: protein motif:HMM:NF039670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6150 family protein"
                     /protein_id="HNF69584.1"
                     /translation="MLRTLIVLAGLLFFISNADAQIIYKTDWKSEAAKKVYVTDMPAE
                     ADIVVFKTEWKSDATEDSGQWYFTEWKDEADLIVYFTEWRSEADLVIYYTEYKEEAGK
                     RK"
     gene            9835..10179
                     /gene="gldC"
                     /locus_tag="PLL28_09435"
     CDS             9835..10179
                     /gene="gldC"
                     /locus_tag="PLL28_09435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HNF69585.1"
                     /translation="MGKTSTIKLNVTLGGDNVPEEITWSADDSGQKAAESCKAFMLSI
                     FDGNADESLRIDLWTNEMRQDEMDKFFFETFATMADTYKRANQQEEVAKFIRDFAFSF
                     GEKVGLLKRKES"
     gene            10232..11011
                     /locus_tag="PLL28_09440"
     CDS             10232..11011
                     /locus_tag="PLL28_09440"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNF69586.1"
                     /translation="MHDKLRIGICLSGGGARGVAHIGVLQALESAGIIPYAVSGCSAG
                     AMVGALYCAGLPPRQIFSTIEKKSIYSIIKMGLPDKGMMELAYFKKVLSENIAHDSFE
                     LLQKPFFISVTNLNTGSAEIIGNGKLIEPVIASQSIPLVFKPQRIGEHLYVDGGVLNN
                     LPIEPLQEICDIIIGVNVTPVRYAENISGMRDVGYRVLNLTLMQNMASRLKQCDYIIE
                     PDTGGYTIFDITKSKTIYDAGYEAALPVAEEIANKHFFNTE"
     gene            complement(11121..>11890)
                     /locus_tag="PLL28_09445"
     CDS             complement(11121..>11890)
                     /locus_tag="PLL28_09445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HNF69587.1"
                     /translation="NNNLDCNDTDILINPDAPEACNGIDDNCNALIDDGLIIYTLYAD
                     ADGDSFGNPSVVVDTCIETLPGYVSNDLDCNDLLASIYPGATEVCNALDDDCNGIIDD
                     NLTFTWLYQDADGDLFGNASVDTLVCMPITGFVSDSTDCDDSNLAIYPGAMELLNGVD
                     DDCDKLIDEGLGLNFYSNGISIYPNPTTGFLSIELNDLKTGVFELYSSSGSLMEIGDI
                     SSRITIIDLQSVPAGIYHLIYKTDTEYSVFRIVKVIE"
BASE COUNT         3442 a         2652 c         2420 g         3376 t
ORIGIN      
        1 aattccatag ccttgctcaa atgcccatcg gtgcgatcga tatagagctt ctcagagccc
       61 tggaaaaatg atggaagaaa taaaattgca tcaaaatcct tctcagtata attcgttccc
      121 aacaatgcgt cagaaaaata tgtggtcttc ccatcaaacg gattttctgc attcgctttt
      181 tgaagatctc ttgatgtctg aaaaaaatat gtcaacgaat ctgcagttaa tattccgcag
      241 aaaataaaaa taaacacata tgataatatc cgctgacgct ttttaatttt cttaaatatc
      301 atccacgaca gcaccgcaat ggtaaccata tatacatagt aaaatcccca agcaaaccta
      361 ccgggcgaac gaaactgttt taatggtgta agtatatcta acagcgattg taatcctaaa
      421 ctaaatggaa gcgacatcga aaataataac atcgcaatgc cggtccacac ataaatactc
      481 aatccctccg gcagcatgat cagcgagcgg cgaatattgc gcttatataa aatgcgtttg
      541 gtaaatatca ccagcaatac gaacagaaat aacaatcctg cataacccac gtatgcatat
      601 ccctcagggt tggcatgtcc cacatgcaga tgtttttgta attgtgtggt aagtgtgcca
      661 tgctttggca agaatactga ttggaacgat gctttatatc tgaaaaaacc atacggtgtt
      721 tccggtctgt cgtgcacatg atcagtaaca tgcatgaatc caagtatcgc cactacggga
      781 atggcagcta atgccattat atacaaggat agtcgccatt tcctgaacga tctgcataaa
      841 atggtcagcg cataaaatcc aatgaacaac gatgcgatca gggcataata catatggatg
      901 aaaccaaaga atatcactac cgccaccagc aacaaagccc tcctgatttg ataattacct
      961 ttctccagtc gtatcatcag ccaccatatc agcggcacaa ataacgcata actcaatgca
     1021 aaatgccccc agaatctatc gagctgtggc gtcatcaacc caatgagcaa agccataccg
     1081 gcagcatacc aggcaggcat gcgataatgc cgcaaaatgt gaaatatcaa taacacgccc
     1141 gcccataatg aaatgaacat caataaatgc aaaatggcaa ccgtattatc atgtacggga
     1201 aacacatgat catcaatcac attgatcaac caggaaatca ttggctgatc atcggtgaat
     1261 accacatgct caccataagg gtaattcatt cccgtaaaac gatcaccctt atcgttggca
     1321 acgtaccaag ccggtgtata ataattcttg aacgcatcgc cgccggtggt gagtaaataa
     1381 tgattgggag aaaaaatgat tttttgccac gccgcacaaa tgatcactgc cgtaaccaat
     1441 gccagtatcc atccgggagc gttgcgtttc atcatcgtgt aaagatgctt cgaaataaaa
     1501 ttttcagaaa gtttcccgat tcctgtaata atatgctcat gcgcatactg ctgaattgca
     1561 cattatcgcg cagtgctatc tcaaccggct ttattttcaa cgatttattt cttgatgcca
     1621 gaaaaataaa ttccaggtcg aataaatatc tgtcaatgct tgtgcgcata aacatatctc
     1681 ttccactgtc attaaatccc tttaaaccgc attgtgtatc cgacactttt aatcgcaata
     1741 ccgttgttgt acatgcgcgc aataattttg aaataaatcg cctgaaagca ggcacatgtg
     1801 cataatatgt cgcgtctttt actccggcgg caacatctgc ttctttattg gcgagcacgg
     1861 tatataattg gataaagctc gcagtcgtat acggaaaatc cacatcggta taaatggtaa
     1921 tatccgcgtt tgatgctgca acaccttgtc gcaacgcata accctttccc ctgttttttt
     1981 gatagttgat acattcaaat ttagcgatcg ccgatttcaa ttgcagcaaa tcttcatcac
     2041 caatgctgcg cgaactgcca tcgttaatca aaattacacg aatatcggca tgtggaatca
     2101 cagcagctat ttcaccatat cccgtaataa tatggtccac ccatgaagga gcgggattgt
     2161 aacacggaag gatgatatcg agcgtgggtt gcgacactta tcgggttgga caattcaaaa
     2221 tttcatgtaa cgcgatttac gggcataaca ataccctata ccgcaggaat aagtttcggt
     2281 aaaactaatg aaataatctg ttgagatttc cccgatcccg ctatcaggca ggtatcttaa
     2341 tgcgatattg ctgtccggct ttaacggtaa gcatatcgga ccttaaccaa ggattataat
     2401 atttcaacat cttgtaattc gtgccattct ttttggcaaa ttccgacagg ttcggaattg
     2461 atgtggtaac tgtaacctcc ttatactgca atgggtcgta caagtcttcc ttggtcagga
     2521 aaaatccata atcctgtgta tgctcataaa taatcttaaa tgccaaagcc ctgaacacat
     2581 agcgcgatgt ttcttcacgg aaatataaat cgtagtacga tttttcatcc tgataattaa
     2641 ccgcgccttc tatcgctccc atgccggcat tgtacgaagc cgctgccagg gtccagttat
     2701 tgaatttcga ataggcatcc ttaaaatact gcgaagccgc ctcacatgat ttctccatat
     2761 cataacgttc atccacttca ccattaattg tcagtccata atgctcaccg gtattttcca
     2821 aaaactgcca atagccatga gcacctgagg gagatatcac attgcgcaat ccgctttctg
     2881 ccattgcgag atatttaaaa tcttcaggca ctccgttttt cttaagggta gattcaataa
     2941 tcggaaacca acgatttgcc agtttcagat tttgaatggt attggaatgc caatatcccg
     3001 taactgtcaa ttcgcgatcc aaacgttcac gcacttccgg atcatcgagc ggcacttctt
     3061 caccggcaaa ggtgagtgtc ttcggcaatt ttgctgcaaa atatcggata gtgttcacct
     3121 catcggcaag tgaacgcaag gtgtctgtag gcatggcaga ttctacatgc gcgccattgc
     3181 catttgcatt tccctttacc gccaatgcaa ctataaggcc tacgcctatc acggcaccaa
     3241 ggatgatata atgtactttt ttcacgcttg ttcttttttc ttgaataccg gcaccgtaga
     3301 gcatggctcg ccatacatga tgcttttagt tacagggcgc aataaggctg ttatttcgag
     3361 ataggcttca gcaggaacgg gtttgtcgct gcatcctttt atcactacgc gttgattgcg
     3421 atattgttct aaatcaactt gcgataactg ctgtgtgtaa taacgtttca tagccagttc
     3481 cggatctccc attactacca tttctgcaat accggcaaca tttgcagcca cgagcatcca
     3541 ggcccaattt ggtatgatgg cgtctgcact gcaataaacg gcaactgttt ttccggcata
     3601 ctgctgccag tcgatagcgg ccaatgcttc gcgaaaatct ttttctcgca gaatgagacc
     3661 gcgaaataaa taatccttca gatcaaaaga cacgatgctt ccttctgcag ggaaaaggtc
     3721 ttccaggtcg aaggtgagta aaccgctttg agctacttta tttactagtt ccatgtccgg
     3781 actttttaga caacaataaa attacatcat ttcgcactcc cgaaaagcga acccgcaagc
     3841 agtgcaatcg taagcaccgg atgcgggttt gcgtatcggt accggaagtg taacgtcgtc
     3901 cggcggaaaa tagtttagtc gccgtagata tatctgttat ctaccacatt ggcgcgctct
     3961 ttcagcacaa tcaataatcc ctgtccctga aatttatttt gcatggaata ggtaagctga
     4021 tttttattca tggtgaagtc agtttgttca ggtgcaggtg tttcctggaa tacctgagca
     4081 acatatacgc cgcgcaatcc tgaaatcgga gcggttattt ttccttgttc tgcagcaaat
     4141 actgcaccta tcacttttgg ttcgcgaccg agaattggtg cacttggtgt tgccatagat
     4201 gcattgagta ctgagtcaac acgtacaccc agtttagatg caatagattc cattgttgca
     4261 ccactgccca ttgcttcatt tattttcttc gtcaatatct cagctttttt ctcaaggatc
     4321 acttcttgct caacctggtc gcgcacatat tcaagatctg caacgccgtt cacgcgaatt
     4381 tctttgaggt atgcaacaac cacgcgatcg gtcatattga aatatttgaa ttcattcaaa
     4441 tctgattcat atgcccaggt aatgatctcg cgtgaatttg gcaaacccgg aacttcgaac
     4501 atgcttttgg taagcggctg tgttgtgcgt ttaaataatt tttcgtcgat cacaccttgt
     4561 tcaaatgatt caggatcctg atgtgcggca taaaaatcat cagccatttt ttcgatggaa
     4621 gaaacagttt ctttgccggg accaatagat ttactgagga acgcaacatt tacaaattct
     4681 tttttattgt gcatttctgt gatttcaata atatgatatc catacatcga tttcacaaca
     4741 ctcacttttc ctaatgagcc tgttttaaat aaataatctt ttatctcagg aacaaaattg
     4801 atagacgggg ttgtccaacc cagatctccg ccttgttgcg cgctgctttg atcatctgaa
     4861 gattcaccgg caattttagt gaaatcacca ccggcgttta ataatcccat gaggctatct
     4921 actatctttt tcgctgcaat gctatcgcgc tgctcgctga tgcctacaag tatgtgacgc
     4981 gattttactg agtcaggcat catagtgcgg ctctttaatg tagccacttt ataatactca
     5041 cctgtttgga atggaccgat gagtgaaccg gcaggacgca caaagaaaga atctacgtat
     5101 gccgaatcaa taccttgtgt ataaagatct tgtctgctgt aattgctatt gccaaaacga
     5161 tcaggatctt cactgcgtgt agcaatgaat gcactgtcgc tctttgctgt tttcattttc
     5221 gcaacacttt ccattaatgc agcaaccgta gctgcgctat ctgctggcga tggaataaaa
     5281 tcccagctga catattctac agtgcgacct tctgtttgct tgtacttatt tttattcttg
     5341 ttgtaatatg tttcgaggtc tttatcattt acaacaattg ttgaatctgc aatttgtgca
     5401 taaggcattg acaccatgcg gaaggcacga cgtgtattgc caacggtata attgcgtttc
     5461 gccatccatt caggaacata tgctgctttt gaaaacagcg acatatattt tgtttgacga
     5521 taatcgcgtt ttgcaatttg ttcgcggcag atgaaattat aatatgcctg acgagcagaa
     5581 ggatcgtttt tttgtgtttc aggatcgctg attgctttca cgtgcaatgg caataatgat
     5641 gcatcataag taccggtttg tgaaagtccg atgtaataat tttttatata cggatgcgga
     5701 tctggaccat aaattaaatt cgtcaattct tcttcggtaa cgccaagacc taattcttca
     5761 agttgcttgt cgatgatttt ctcattcaca tattcagtcc atgattgttc attgagacca
     5821 aattttgtct gagggtctaa ctgcatagaa ccgttttcat attgcaacac ctgaccagtt
     5881 tgaagtgtga gatattccaa taatgttctg ccgcttcttt cggcaacttc atcacggttt
     5941 acttcttcac catacatagt tgcataatcc ggggctttat ttccacggaa catacctgtg
     6001 tttgaattag tggcatcctg caccagaaag ccaatcaaac acaatgcaat aaggccaact
     6061 gcgagccacg aatactgacg gatttttgaa ataagcatat gctattcgaa taatattaga
     6121 atattgggga tgcaaaggta gtggatggcg tgtggaaaac aaaactatcg cccgtggtat
     6181 agcactttca acctcaaaaa gggggtaaaa tgtgccggta cgcctgaaaa acactaaaat
     6241 caggctttgg gagcctctgt ttcgccggat ttggaagccc cggcctgctt tttggccatt
     6301 tcctcctgtt tgcgatagtg ttcttccaaa tacagtctgt ttcttttttt gaagcggaac
     6361 atgaagtagg ccaccgaacc ggtaaacaac gtaatataga tgtagttgcc gtattgctga
     6421 ccgttcaacg ctcttgtaat ggccattacc agtgccacgg cagccgtcac cagccacatc
     6481 acctcaaaga tcttcatgag gcgcacatac atattgggag cttccttctt cgccataata
     6541 attcgtcaaa attacgtctt gttagcggat ttcccctgtc gccatgcttc ctgattaata
     6601 tcttcgcacc atgcaattgg tactggatct cggcaatacg aagcataaat atgccgttta
     6661 tgaaggcaat gagctcgtaa ggctgggcag cgaaccgcaa ttgacagcgg aaataacggc
     6721 aaacctcatc gatatctatg gtatagatca agccattgtg agcagtgtag accatttcga
     6781 tgaagccctg gaatccttgc tggaagagcg taccggcctg cttcagttca cgcataccac
     6841 ccctttaccc atcatcaatc tctacggcac ccccgcaacc ctgggaaaag acagacttgc
     6901 cggagcagtt ggcgcacata ccgccttcaa aggctctcct gttttggtgg tggatgccgg
     6961 cacctgtatc aaatatgatt ttctggataa ggaaggtatc tactacggcg gagccatttc
     7021 tcccggcctt ttcatgcgat tcaaggctat gcacaatttt acggcgcgac tgccgttaat
     7081 agacccggaa aactacgcaa ccgtgttaga agtgccgctg acagggactt ctacggtcga
     7141 ctcgctgcgg tcaggagccc tgaacggagc attgtttgag gtggaaggtg ctatcagcgc
     7201 ctacacagga caacatgcgg gattagcagt gatacttacc ggcggagatg cgggtttctt
     7261 tgaattgcat atgaaaagca ggatatttgc acgcccaaat cttgtgctgg aaggcctgca
     7321 caccattttg aattacaatc tgaatcactg atgcacctcc gaatactacc gctgttagcc
     7381 cttacaacac tgaccctctc catttctgca caagtaaatt ccccttactc acgatacggt
     7441 ctcggtaata tgtacccggg aagttttacc gctgcaaacg gcatgggcgg attttctgca
     7501 gccaatttct ctccaagcgc aattaatttt ctcaacccgg cttcatttgc agaaatcggt
     7561 ggcgcctctt tcgatgttgg cgtatatggc acggtgatgt cactcaacac aaattccgaa
     7621 acatttactt ccggcgatgc aaacttgtca aatatggcat tcgcatttcc ggtatataaa
     7681 aatttacgtc gcgacagatt tggcttaagc tttggattag tgcccttcag caccttccaa
     7741 tatgatatcc tcgacgaaac agataccggc gatccggatt tgggaaccaa acaatataat
     7801 tatgttggcg acggacaatt atatcaactt tacggaggct tcggttatcg tcatcaaaca
     7861 gatactgttt ctcaggtaaa aagtattcaa ggccgacaag atactgttat ctcggcagga
     7921 tttctgagca ttggcgccag ctccgcatta ttgttcggct cattatataa tactacttat
     7981 gcttcgtttc ccgatatcct gaattcgcag acaacaaaat tgtcgcgcga caatgatgtg
     8041 aacggcggca tgtataatat tggtgttgga tatcaaaaac aatatgtgcg caaacatggc
     8101 acaaaacgcg atatcttaaa ttggaaattc ggcggtactt tttcaccaca agttgatgta
     8161 catggtacgc aatctatctt gtggaccaat attttgaaat atggtaatta cgaaactgta
     8221 accgacacat tatatacggc accggataca tcaggaaata ttcatatgcc tatgtcttat
     8281 tctgccggtt tatcctttag tttcttcagt agtgatgatg aaaataaaaa tcaattcacc
     8341 ctgggattgc aatacgcttc taccatgtgg agtcaatatg acggatttca ggatgcaggt
     8401 gaattagcgg atagctggcg cgctacattg ggtgcagaat ttattcctaa aaagaagcaa
     8461 gatcaaaatg cgccactgat gtatcgcttc ggcgcatata caggtaaaag caatctgatt
     8521 atcgacggtg tgcaattaaa tgatgttgga ttttctgccg gacttgccat tcctatgggc
     8581 tatggcgaaa acgtgttgcc atattatcgc aattctaaaa ttaatatgtt cctgaatgtg
     8641 ggacagcgcg gagcgaatgc cgatatcagt gaaacatatt tcaacttcgg cgtaggcttc
     8701 agcatgatag accttggttg gtttatgaaa tataagctga actgacgcat atagcgtaaa
     8761 tttatcctgt gatcacttca acatggatgt cgaaataccc gcttacttta ttagtggcga
     8821 tgacgatttt tgccggctgt gtaaataatg taaatgaggt gaataaagcc acgcaactgg
     8881 cagagccggg aattgaatac ggcaaagaca tcgaattatt tttcagcgaa gatggcgaag
     8941 tgaaaattcg cgttacagca cctactgttg tgcggcacaa taccgaagac ccttacaccg
     9001 aatttaccga tggccttaaa gttgaatttt acaacgacag catgcatgtt gacacctggc
     9061 tgacggcaaa ctcgggtaca cggtacgaag cacaaggaag aactgtttta caaaatgatg
     9121 tgcaggtgtt gaataaaaaa aatgagcatc tgagcacaga agaattaatc tgggatgaaa
     9181 aacgccatat catttactcc gataaatttg tgaaaatcac cactcccgat caggtattat
     9241 atggcgatgg catggaagcc gatgaacaac tcacgcgcta caccatcaaa aaacccagag
     9301 ggacgattca ggcggaaatg gagtgattaa ttagatgatg caagtgtgca gcaacttttg
     9361 ggccacctct gcgcctctgc gcctctgcgt gaaaaaccct ccgcgccaca cctatttccg
     9421 cttccctgct tcttccttgt attctgtata gtagatcaca aggtcagctt cagagcgcca
     9481 ttctgtaaaa tacacgatca aatcggcttc atctttccat tcggtgaaat accattgtcc
     9541 gctgtcttcc gttgcgtcgc ttttccattc ggttttaaac accacaatat ctgcttcagc
     9601 aggcatatcg gttacatata cttttttggc cgcttcactc ttccagtcag ttttataaat
     9661 aatctgagca tcagcattgc tgataaaaaa cagaagtcct gccaatacga ttaatgtgcg
     9721 tagcatttcg ggtttaggtt tggttaaagt tcgtcagttt tgggcatctc gccaaatagc
     9781 aaagagcccc ataccatcat ctttgactaa tttcgtgcca aaatagcaac aaccatgggt
     9841 aaaacctcaa ctataaagct caatgtgacc ttaggcggcg ataatgttcc tgaagaaatc
     9901 acctggagtg ccgatgacag cggtcaaaag gctgccgaaa gctgcaaggc cttcatgctc
     9961 agtatttttg atggcaatgc cgatgaatcg ctgcgcatag acctgtggac gaacgagatg
    10021 cgccaggatg aaatggataa attcttcttc gaaacctttg caaccatggc cgatacttac
    10081 aaaagggcta atcaacaaga agaggtagca aaattcatcc gcgattttgc attttcattc
    10141 ggagaaaaag ttggattgct aaaacgcaaa gaatcctgaa tttttttagt gtatataccg
    10201 tttacaatgc agatacagta tatttactga tatgcatgat aaactgcgca taggtatttg
    10261 tttgtcggga ggcggcgcca gaggtgttgc acatattggc gtactgcagg cattagaaag
    10321 tgcgggtatt attccatacg ccgtaagcgg gtgcagcgca ggtgcaatgg tgggtgcact
    10381 gtattgtgcc ggattgccac cgcgacaaat tttcagcacc atcgaaaaga aaagcattta
    10441 cagcatcatt aaaatgggac tgccggacaa gggcatgatg gaacttgcgt attttaaaaa
    10501 agtgctttca gaaaatattg cgcacgacag ttttgaatta ttgcaaaagc cattttttat
    10561 ttccgttacc aatctcaata ccggcagcgc agaaattatt ggcaatggca aattgataga
    10621 acccgtaatt gcatcgcaat ctataccact ggtatttaaa ccacagcgca taggagaaca
    10681 tttatatgtc gatggcggtg tactcaataa tttaccgata gagcccctgc aggaaatttg
    10741 cgatatcatt attggtgtga atgtaacacc ggttcgctat gcagaaaata tcagcggcat
    10801 gcgcgatgtg ggttaccgtg tgttgaatct cacattgatg caaaacatgg cctcgcgatt
    10861 aaaacaatgc gattatatta ttgaacccga tacaggagga tatactattt tcgatatcac
    10921 caaatcaaaa acaatttatg atgcgggata tgaagcggca ttgcctgtgg cggaggagat
    10981 agcgaacaag cattttttta atacagagta gctcagcatt ataaattttg atggagctcc
    11041 ggttttatta gatcaaacta aaaaagttta aattaattcc tagtgtccaa ttaattgggt
    11101 aatgcgtact agctaaaaaa ctactcaata actttgacaa ttctgaaaac tgaatattcg
    11161 gtatcagttt tatagatcaa atgatatata cctgccggta ctgattgcaa gtcaataatt
    11221 gtaatccttg aggaaatatc tcctatttcc attagagagc cgctagagct atacaactca
    11281 aaaacaccgg tttttaaatc atttaattca attgacaaaa acccggtagt tgggttcgga
    11341 taaatagata ttccattcga atagaaattc aagcctaatc cttcgtcaat caatttatca
    11401 caatcatcat ccacaccatt gagcaattcc attgcgccgg gatatattgc taggttactg
    11461 tcgtcgcagt cggtggagtc agaaacgaat ccggtaatag gcatacaaac cagagtatca
    11521 acactagcat taccaaataa atcaccatct gcatcctgat ataaccatgt gaaagtcaaa
    11581 ttgtcgtcaa ttatgccgtt acaatcatca tcgagcgcat tgcatacctc tgtggcgccg
    11641 ggataaatcg atgccaataa atcgttacaa tccaaatcat ttgatacata acccggcaag
    11701 gtctcaatgc aagtgtcaac tactactgac gggtttccga aactgtcgcc atctgcatca
    11761 gcgtaaagtg tatatatgat caatccatca tcaattaaag cattgcaatt atcatcaata
    11821 ccattgcatg cctccggggc atcagggttg attaaaatat cagtatcatt gcaatccagg
    11881 ttgttgttta
//
LOCUS       DAOQHY010000104        11707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2708_length_11707_cov_5.325781, whole genome shotgun sequence.
ACCESSION   DAOQHY010000104 DAOQHY010000000
VERSION     DAOQHY010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11707
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2708_length_11707_cov_5.325781"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1771)
                     /gene="ppdK"
                     /locus_tag="PLL28_09450"
     CDS             complement(<1..1771)
                     /gene="ppdK"
                     /locus_tag="PLL28_09450"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNF69588.1"
                     /translation="MSKQYVFGFDSGDGKNKKLLGGKGANLCEMTQIGLNVPPGFVIS
                     TEACLAYLDTSEHVLPEGVIEQVRSQMAAIEKASGKRFGDPENPLLVSVRSGSAMSMP
                     GMMDTILNLGLNTDTLKGLITQTGNERFGYDAYRRFIQLFGKVALGVPDEKFDVVFED
                     IKHNAGVKADIGLNTNNLKEASERFLKVVAEHTGRPFPADVYTQLEIAIKAVFNSWMG
                     KRAVDYRREFKITPEMANGTAVNVVTMVFGNMGNDSATGVGFTRDPGTGENVMFGEYL
                     TNAQGEDVVAGIRTPKAVQMLAKEMPEQYKQLEELRNKLESHYREVQDFEYTIEKGVL
                     YCLQTRNGKMNTTAMVRTSVEMAREGIISRSEALMRIKPAMLEQLLHPRLDSLQKHEP
                     LAQGLPASPGAASGHVVFDADRAEMLGRGGEKVILVREETKPEDIHGFFASQGILTSR
                     GGKTSHAAVVARGMGKPCVAGAEGIKVDVKMRQATIGEKVLHEGDYITIDGGTGFVYK
                     GEIPTVEPTFSDELKILLGWADEAAKLKVFANADTPEAAKKALHYGAMGIGLCRTERM
                     FNAPERLPIVVEMILANTTPEREK"
     gene            2070..3017
                     /locus_tag="PLL28_09455"
     CDS             2070..3017
                     /locus_tag="PLL28_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69589.1"
                     /translation="MKRTTLVALVCLLTGVMRAQTPSDAVMMNPGEICIDLHYGHSAW
                     DEYWEGDSLRDNGNIGTLNTSIYGTGFMLGLINRVNLLASLPYVVTNPTGGVISGDKG
                     IQDASFYLKGLLVEQKLGKGTFKTLASIGISLPAGDYVPDAPFAIGLGCPDGIFRGIL
                     HYDADMGLYGRVDAGYHLRGSAYLDRTYYYTTQAYYSDQVDMPNATDYNVTVGYITKN
                     KHFKAEAVYGGLTTFDGFDIRRQDGGFPSNDMEMSRVGVNLEYYDLFVKGLAIHAGGG
                     MVLTGSNVGKSTMFGGAISYQFPLWDGRDNKKSETPEAK"
     gene            3020..4570
                     /locus_tag="PLL28_09460"
     CDS             3020..4570
                     /locus_tag="PLL28_09460"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNF69590.1"
                     /translation="MKKLNYLVISGLAVSAFMASCTEDLPQYVGFEPYTYASLDEAGG
                     TWQPILLTSGADTTLTAPVDVTDPSYISELEEVKGYNGSLSADEQEAVDYWGNNTVIR
                     WQEIAQELIAKYNLAPSPNADGSYSSPSSLNPGVYPYFPFAHPPYATRVYAYLGAAQY
                     DALITAWNMKYTYNRPAPYDVDNSISPEYPDNTLPSWPSEDATVAEVSSELLKFLFPL
                     EVDYLNEMAAECRNSRKWAGMNVESDLVAGDAIGGFVFRKYKARAANDSMKFAQVDAA
                     TYNVMEAAAEATWGSMWPHWENLEVPQRPVGITPKYSSVTTWWIPSLEAVRPGPPPAM
                     NSEEYKAAEAELLDLTKNCTREQERIAYNWGDGFGTNTPAGHWGKIAADYMVSYRMNP
                     LRTARTYAYMNTAMMDAGISCWDTKYFYFYPRPPQANPEIKTLLGLPNFPSYTSGHAT
                     FSAAAGAVLAHIFPAEASEINGYVDEATESRIYGRIHFRFDCESGKTAGTSIGGYAVD
                     RMMTDGAE"
     gene            complement(4654..6441)
                     /gene="argS"
                     /locus_tag="PLL28_09465"
     CDS             complement(4654..6441)
                     /gene="argS"
                     /locus_tag="PLL28_09465"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNF69591.1"
                     /translation="MTNLLEQLQKEVASVAEQLYATSFDAASIKIDKTPPDFDGEFTV
                     VVFTFVKASRKSPEATANELGAALMAGFSLIKSFNVVKGFLNISFTDDCWLQILSEIH
                     SAENFGQLPSTGKQVVVEYCGPNTNKPLHLGHIRNILLGYSTANILSAAGHSVHKVNI
                     LNDRGIAICKSMVAYKRNGNGSTPASIGMKGDHFVGKYYVEYSKIIEAEISALMNDTD
                     ISKDAAEKQAPIYLEAQEMLLKWEAGDSEVMALWNMMNSWVYSGFDITYKLIGIDFEK
                     EYKESEFYLNGKKMVLEGLNSGAFFKKEDGSVWVDLASDGLDQKVLLRADGTSVYITQ
                     DMGVAEERYRDFTFDESIYVVANEQDYHFKVLKLVLQKLGRKYADGIHHMSYGMVDLP
                     EGKMKSREGTVVDADDLIEGMVSEAMDFLASSEKNAELNETEKQALAHDIGMSALKYF
                     ILKVDPKKRILFNPKESIDLHGNTGPFIQYAYARIRSILRKIDEVKVNIQVSIGTGLM
                     PAEKELIIILSQYPGIVKLAAETYSPAEIGNYLYLLAKKFNSFYADHSILKAESEEKM
                     HLRAVIALHVSLVIKHGLDLLGIHSPEKM"
     gene            complement(6438..7391)
                     /gene="rocF"
                     /locus_tag="PLL28_09470"
     CDS             complement(6438..7391)
                     /gene="rocF"
                     /locus_tag="PLL28_09470"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HNF69592.1"
                     /translation="MRRVKVISVNSELGAGTRGAGLGFDAIRVAAWTKGSRYFKEHPP
                     LIIQSNNDEVLDDIETAYSVKIQFIVEMYKKISEAIVESMKKKEFPIIISGDHSNAGG
                     TIAGLKMAYPKETLGVIWIDAHADLHSPYTTPSGNLHGMPLATATALDNKVCQINQPE
                     KKTIEAWEKLKKLGGISPKITFKDLVYIAARDMEKAEKFILKKNNVRIYSVQKMRETG
                     IQSVIEDALKRLAHCDRIYVSFDVDSMDANVSRGTGTPVKSGLYESEAFSLMVALAKQ
                     EKVCCMEITEVNPTLDNKKNLMAEIALEILEQFTAEVEKRL"
     gene            7466..8875
                     /locus_tag="PLL28_09475"
     CDS             7466..8875
                     /locus_tag="PLL28_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HNF69593.1"
                     /translation="MKNTYLDLIQQTFYFPQDGFEVKNNYLYFNGIPLKELIDKYETP
                     MRITYLPKISDNIQRAKRYFNDAIRKLNYPGTYNYCYCTKSSHFAHVMEEALKNDIHL
                     ETSSAFDIDIIEKLYLNKMVTKDTLIVCNGYKTAIYTQRISKLANLGFNITPVLDNKE
                     ELDAYQHTIIDSCDIGIRIASEEQPEFQLYTSRLGIRYSEIRDFYLNKIKTNPKFRLR
                     MLHFFINSGIKDNIYYWNELNKSLKVYIDLKRVCPELDGLNIGGGWPYRNSLGFEYDY
                     PYMAEEILNRIKQTCEEEKVECPNIFTEFGSFTVAESGAFIFQVLGEKRQNDSERWYM
                     IDNSLMTTMPDSWGINSKFILLPVNKWGNEVQRSNIGGLTCDQMDYYNSEAHTNELYL
                     PINTMGEPLYIGFFHTGAYQESISGYGGTKHCLIPSPKHILISRGEEGELKYEVFADE
                     QQVESMLTVLGYDKLDERD"
     gene            complement(8872..10212)
                     /locus_tag="PLL28_09480"
     CDS             complement(8872..10212)
                     /locus_tag="PLL28_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69594.1"
                     /translation="MISVQNRRQPLSGNNKYIIVLLATVFLFASCGIFNGAQHDSTVS
                     HPTNTDQTTVHADTVIRKKVDSVIIPTKVIDNLASEKKPAYDISFVLPFSIDAAELEK
                     LSSEENITGFQPLASIEFYEGALMALDTLDSLGIKLNVHVFNNYKDSSLTAALFSKPE
                     IMKSDAVFGPIFNESLKAASPVAQQNKFYLVSPLSPNNSFIDTNKYFMMANPTVEAQL
                     EAMLKSIETQHPFANIINIYRSDKPNEKHLADDFAAAFNIANTGKGMRLSNATSYAEA
                     TALMKSVENFVFISSYDELYVNGLIRDLSKFSRETPVEMLGLQHILSFESISIDYYEN
                     LHFTYPNAYYIDRNSPKVINFETAFIERYSTRPSDFACRGYDFTLYIAMMLNSYGVDI
                     SASADKLNPATPFMLDPVRFTGIKNNAGITRYMEKSNITILRYDQYIFKPLSTN"
     gene            complement(10169..>11707)
                     /gene="guaA"
                     /locus_tag="PLL28_09485"
     CDS             complement(10169..>11707)
                     /gene="guaA"
                     /locus_tag="PLL28_09485"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNF69595.1"
                     /translation="TEKILILDFGSQYTQLIARRIRELNVYSEIVPCTKIPEITPDIK
                     GIILSGSPFSVSSPEKPAFDIEAILDKRPILGVCYGAQFVAKHFGGVVQPSSKREYGR
                     AFLHIQDRNCELFYGIDDESQVWMSHSDTVMQVPAGFHVTAKTASIDVAAFASDPGRF
                     NNPMYCIQFHPEVTHSEQGKKMLQNFVVGTCGCHQTWTPESFVEETVAYLQTTIGSDQ
                     VVLGLSGGVDSSVAAILLNKAIGKNLISIFVNNGLLRKNEFNSVLHSYQDMGLNVIGV
                     DAEDEFLSALEGESDPEKKRKIIGKVFIDVFERESKKLTNVKWLAQGTIYPDVIESVS
                     VHGPSATIKSHHNVGGLPEKLHLKVIEPLRFLFKDEVRKVGDTLQLKHEILHRHPFPG
                     PGLAIRIIGDITREKVRIVQEADAIFINHLKSSGWYDQVWQAGAILLPVQTVGVMGDE
                     RTYENVVALRCVNSTDGMTADWSHLPHTLLAQISSDIINHVKGINRVVYDISSKPPAT
                     IEWE"
BASE COUNT         3181 a         2716 c         2355 g         3455 t
ORIGIN      
        1 ctttttcacg ttccggtgtt gtgtttgcga gtatcatctc cactacgatt ggcaggcgct
       61 caggcgcgtt gaacatgcgt tctgtgcgac aaagtccaat acccatggca ccgtagtgca
      121 aggctttttt agccgcctca ggggtatcgg cattagcgaa tacctttaac ttggcagctt
      181 catccgccca tccgagcaga atcttcaatt catcgctgaa ggttggctca acagttggaa
      241 tctcgccttt atacacaaat ccggttcctc catcgatagt aatatagtcg ccttcgtgga
      301 gtactttttc gcctattgta gcctgacgca tcttcacgtc aacttttatt ccctcggccc
      361 cggcaacgca tggttttccc atacctcgcg caactactgc ggcgtgtgag gtctttcctc
      421 cgcgagaggt aaggataccc tgactggcaa agaatccatg tatatcctcc ggtttggtct
      481 cctcgcgcac caggatcacc ttctccccac cgcgaccgag catttcagcc ctgtcggcat
      541 cgaaaacaac atgtccggag gctgctcccg gagaggctgg taaaccctgt gcaagcggct
      601 catgcttttg caggctatcc aggcgtggat gtaacaattg ctctaacatg gcaggtttta
      661 tgcgcatcaa tgcctctgag cggcttataa tgccttctct ggccatttca acgctggttc
      721 ttaccatggc agttgtgttc atcttgccat ttcgcgtttg cagacagtac aggacgcctt
      781 tttcaatcgt atattcaaaa tcttgcacct cgcggtaatg actctctaac ttgttgcgta
      841 gctcctccag ttgcttatac tgctcaggca tctctttagc cagcatctgc accgcttttg
      901 gcgtgcgaat tcctgctacc acatcttccc cctgcgcatt ggtcaaatat tcaccaaaca
      961 tcacattttc tcctgtcccc ggatcgcggg tgaaaccaac gccggtggca ctgtcatttc
     1021 ccatgtttcc aaaaaccatc gttaccacgt tcaccgcagt tccatttgcc atctcaggtg
     1081 tgatcttgaa ttcgcgacgg taatcaaccg ctctttttcc catccaactg ttgaatacag
     1141 ccttaatagc aatctctagt tgagtatata catcagcagg gaatggcctg cctgtatgct
     1201 cggcaactac cttcaaaaat cgttcagatg cttcttttaa attattggta ttcaagccta
     1261 tatcagcttt aactcctgca ttgtgtttga tatcttcgaa tacgacatcg aacttttcat
     1321 cgggaacgcc aagtgctact tttccaaaca attggataaa acgacgataa gcatcgtatc
     1381 cgaatcgctc gttgccggtt tgggtgatga ggcctttcaa ggtatcggtg ttaagtccca
     1441 ggttgagaat agtatccatc atcccgggca tcgacatggc tgaaccggac cgcactgata
     1501 ctaataaggg attttcagga tcgccaaaac gctttccact tgctttctcg attgccgcca
     1561 tttgcgacct tacctgttct attactcctt caggcaatac atgctctgag gtgtcaaggt
     1621 aggctaaaca ggcttccgtg ctgatgacga atccgggcgg cacatttaaa cctatctggg
     1681 tcatctcaca caggtttgcc ccttttcctc ctaacaattt tttgtttttt ccgtcgccgg
     1741 aatcaaatcc aaatacatat tgcttgctca tatcatggta ttttgaagtt acttagaatg
     1801 agggctaagt tagattgatg ccaacctgcc ggaaatgact tgggtttcct atcatagaag
     1861 ctaaagtcat catttaaagt acacaagcgt acactcgaat ccgctattta gatattgccg
     1921 acgaaaactg aagaactagt ttattaaacc ttaaaagaag ttcaaactct ttaaaactaa
     1981 tgtaaactat ttagctcaaa accttatgcc atctattttt gaccgcatta aaagacaact
     2041 actaaacctt attgatttaa atgaatctta tgaaaagaac tacactggtg gcgttagtat
     2101 gcctgcttac gggtgtaatg cgcgctcaga ctccttctga tgccgttatg atgaatcccg
     2161 gagagatctg catcgacctg cactatggtc attccgcttg ggatgagtat tgggaaggcg
     2221 attctctgcg cgacaatggc aatatcggta cactcaacac aagtatttat ggcacgggat
     2281 ttatgctcgg actcattaac cgagtgaacc tgctcgcatc tttaccctac gtcgttacca
     2341 accccacagg cggggtgatt tccggcgata aaggaatcca agacgcatct ttctacctaa
     2401 aaggacttct ggtagaacaa aaactaggca aaggtacctt taaaaccctt gcaagcattg
     2461 ggatctcatt gcctgcaggt gattatgtgc cggatgcccc atttgctatc ggcctcggct
     2521 gtccggatgg tattttccgc ggtattcttc attacgatgc cgacatggga ttatatggcc
     2581 gcgtagatgc aggctatcac ctccgtggct cagcctacct cgatcgcacc tattattaca
     2641 ccacgcaagc ctattactca gatcaagtag acatgccaaa tgctaccgat tacaatgtaa
     2701 cagtaggcta catcaccaaa aacaagcact tcaaagcaga ggctgtttat ggcggattga
     2761 caactttcga tggcttcgat attcgtcgtc aggatggcgg attcccttct aatgatatgg
     2821 aaatgagccg agtgggtgtg aatcttgaat actatgacct gtttgttaaa ggtctggcaa
     2881 tacatgccgg cggcggaatg gtgcttacag gaagcaatgt aggcaagtct accatgttcg
     2941 gcggcgctat ttcttaccag tttcctttgt gggacggtcg cgacaataaa aaatctgaaa
     3001 cccctgaagc aaaatgatca tgaaaaaatt aaactatctc gtaatatccg gactcgctgt
     3061 ttcagcattt atggcatcgt gtaccgaaga cctgccgcaa tacgtgggtt ttgaacctta
     3121 tacctatgcg tcattggacg aagccggcgg aacatggcaa cctattttat taactagtgg
     3181 ggctgataca accctaactg ctcctgttga tgtaactgat ccgtcttata tttctgaatt
     3241 ggaagaagtg aaaggctaca atggctcact ttcagccgat gagcaggaag ctgttgatta
     3301 ttggggcaat aacaccgtaa ttcgttggca ggaaattgca caagagctca tagcaaaata
     3361 caaccttgca ccatcaccta atgctgatgg atcctatagc tccccaagtt cattgaatcc
     3421 cggcgtttat ccttatttcc cgtttgcgca tccaccatat gccacgcgtg tgtacgcata
     3481 cctgggcgcc gcgcaatatg atgctttgat cactgcgtgg aatatgaaat atacttacaa
     3541 tcgtcctgca ccttatgatg tagacaacag catctctcct gaatatcctg ataacaccct
     3601 gccttcatgg ccttcagagg atgctaccgt tgcagaagtg tcttccgaat tactcaaatt
     3661 cctcttccct ttggaagttg attacctgaa tgaaatggct gcagaatgtc gcaattcacg
     3721 caaatgggca ggtatgaacg ttgaaagcga cctggttgcc ggcgatgcca tcggaggatt
     3781 tgttttccgt aaatacaagg cgcgtgctgc caacgacagt atgaaattcg cacaagtaga
     3841 tgctgcaact tacaacgtaa tggaagccgc tgccgaagct acctggggct ctatgtggcc
     3901 acattgggaa aaccttgaag tacctcaacg cccggtaggt attacaccta aatatagctc
     3961 tgtaactacc tggtggattc cttcgcttga agcagtgcgc cccggtccgc cacctgccat
     4021 gaattctgaa gaatataagg ctgctgaggc tgaattgtta gacctcacca aaaattgtac
     4081 tcgtgagcaa gaacgcatcg cctataattg gggtgatggt ttcggcacca acactccggc
     4141 aggccattgg ggaaaaattg ctgccgatta catggtaagc taccgcatga atccattgcg
     4201 aaccgcaaga acttatgctt atatgaacac tgccatgatg gacgccggta tctcatgctg
     4261 ggatacgaag tatttttatt tctaccctcg tccgcctcag gcaaatcctg aaatcaaaac
     4321 actgcttgga ttaccaaatt tcccatccta tacttccggc cacgcaacgt tttcagcggc
     4381 cgcaggtgca gtgttagccc atattttccc tgctgaagca tctgaaatta atggatatgt
     4441 tgatgaagct acagaatcaa gaatttatgg tcgcattcac tttaggtttg attgcgaaag
     4501 cggtaaaact gccggtactt ctatcggtgg atatgccgtt gatcgtatga tgactgacgg
     4561 agctgaataa actttccctg tttatcatat atgaggtcat ccttcggggt gacctctttt
     4621 tttgtgggaa ggattgcaac gtgtaggaag aaattacatc ttctcagggc tgtgtatacc
     4681 caacagatca agaccgtgtt tgatcactaa tgaaacatgt aaggctataa ccgcacgcaa
     4741 atgcattttc tcctccgatt cagctttcaa aatgctgtga tcggcgtaga aggaattgaa
     4801 ttttttcgcc aataggtata aataattgcc tatttcagct ggtgaatagg tttctgctgc
     4861 aagctttacg atgcccggat attgactcaa gataattatc aattcttttt ctgcaggcat
     4921 caaaccggtg ccaatcgaaa cctgaatgtt aaccttaacc tcatcaattt tccttaaaat
     4981 actcctgatt ctagcatacg catattgaat aaaaggtcct gtgttcccat gaaggtcaat
     5041 agactccttc gggttaaata atatgcgctt tttaggatct accttcagga tgaaatattt
     5101 gagtgccgac atgccaatat cgtgcgccaa tgcctgcttt tcggtttcat ttagctctgc
     5161 attcttttct gaggatgcaa gaaaatccat cgcctcacta accatacctt ctatcaaatc
     5221 atcggcatct accaccgtgc cttctcgact tttcattttg ccctcaggaa gatccaccat
     5281 gccgtagctc atgtgatgaa tgccatcagc atatttcctt cccaattttt gcaataccag
     5341 tttgagtacc ttgaaatgat aatcctgctc gtttgcaact acataaatgc tttcatcaaa
     5401 agtgaaatcg cggtatcgct cctctgcaac acccatatct tgcgtgatgt aaacgctcgt
     5461 tccatcagcc cttaacaata ccttttggtc aagcccatcg gatgcgagat caacccagac
     5521 actgccatcc tcttttttaa agaaagcgcc actgttcaat ccttcgagca ccattttttt
     5581 accattgaga taaaattcgc tctccttata ctctttctcg aaatcaatgc cgatcagttt
     5641 ataggtgatg tcaaagccgc tgtacaccca gctgttcatc atattccaga gagccattac
     5701 ttcagaatct cctgcttccc atttcaaaag catttcctgt gcttcgaggt aaattggagc
     5761 ctgtttttcg gctgcatcct tgctgatatc tgtatcattc atcaaagcag aaatttccgc
     5821 ttcaatgatc ttgctatact caacataata tttgcccaca aaatgatcac ctttcatccc
     5881 gatagatgcc ggggtgctgc catttccatt gcgcttgtaa gctaccatgc tcttgcaaat
     5941 tgcaattcca cggtcattca gaatattaac cttatggaca ctgtgtcctg cggcgcttaa
     6001 aatatttgca gtagaatacc ctaacagtat attacggata tgtcctaaat gcaagggctt
     6061 attagtattc ggaccacaat attcaactac gacctgtttg ccagttgaag gaagctgacc
     6121 aaaattttct gcgctatgaa tctcgcttaa tatctgcaac caacaatcat cagtgaagct
     6181 gatattaagg aaacccttaa ccacattaaa tgatttgatt aggctgaaac cggccatcag
     6241 ggcagctccc agctcatttg ccgtagcctc ggggcttttg cgacttgcct ttacaaaggt
     6301 gaaaactaca acagtaaact caccatcaaa atcaggtggt gtcttatcaa ttttaatcga
     6361 tgccgcatcg aatgatgttg catataattg ctctgctaca gaagctactt ccttctgtaa
     6421 ctgttccaat aaattggtca taaccttttc tccacttcag ccgtaaattg ttccagaatt
     6481 tccagcgcaa tttctgccat caaattcttc ttgttatcaa gtgtcggatt cacctcagtg
     6541 atctccatgc aacatacttt ttcctgtttg gctagtgcta ccatgagcga gaatgcctca
     6601 ctttcataaa gaccgctctt tacaggtgtt ccggttccgc gtgaaacgtt ggcatccata
     6661 ctgtcgacat caaaggatac ataaatccga tcgcagtgtg ccaaacgctt gagtgcatcc
     6721 tcaatcactg attgtatgcc tgtttcacgc attttttgta cggaataaat gcggacatta
     6781 tttttcttga ggatgaattt ttcagccttc tccatgtcgc gcgcagcaat gtaaacaagg
     6841 tctttgaagg tgatttttgg tgagatgcct cccagctttt tcaacttctc ccaagcttct
     6901 atcgttttct tctcaggctg gttgatctga caaaccttgt tatctaatgc tgtggcagtg
     6961 gccaatggca taccatgcaa atttcctgat ggggtagtgt aagggctatg cagatcggca
     7021 tgagcatcta tccagattac tcctaaggtc tccttcggat aggccatctt caatccggcg
     7081 atagttccac cggcgttgct gtgatcgcca ctgatgataa tggggaactc cttcttcttc
     7141 atcgactcca caatggcctc agagatcttt ttgtacatct ccacaatgaa ctgtatctta
     7201 acagaatagg ctgtttcgat atcgtcgagc acttcatcat tattgctctg gatgatcagc
     7261 ggagggtgct ctttgaagta cctgctgcct ttcgtccatg cagctaccct gatggcgtca
     7321 aatcctagtc ccgccccgcg ggtacctgcg cctaattcgc tgtttaccga gataactttt
     7381 actcgtctca taccatgcaa agttattatt tttgtgctgc taaagtaatc tatcgttttt
     7441 tcacgtaaaa tcaaccactc agacattgaa gaacacttac cttgacctca tccagcaaac
     7501 cttctacttc ccgcaggatg gattcgaagt aaaaaacaat tatctctact ttaacggcat
     7561 cccgttgaaa gagttaatag acaaatatga aacgccgatg cgcattacat atttgccgaa
     7621 aatatctgat aacattcagc gcgctaaacg ctattttaat gacgcgatcc gcaagctgaa
     7681 ttatcccgga acatacaatt actgctattg taccaagagt tcgcattttg cgcatgtgat
     7741 ggaagaggca ctgaagaatg atattcacct cgaaacatca tctgctttcg atatcgatat
     7801 catcgaaaaa ttatacctga ataaaatggt taccaaagac acacttatcg tgtgcaatgg
     7861 atacaaaacg gccatttata ctcaacgtat cagcaagctc gcaaacctcg gatttaatat
     7921 cactcctgtt ttagataata aggaagaact agatgcctat caacatacca ttatcgatag
     7981 ttgtgatatt ggtatacgca tcgcttctga agaacaaccg gaattccagt tatatacgtc
     8041 gcgactcggt attcgatata gtgagattcg cgatttttat ctgaataaaa taaaaacaaa
     8101 tccaaaattc cgtttgcgga tgttgcactt ctttatcaat tcaggaatta aggataatat
     8161 ctattactgg aatgaattaa ataaatcgct gaaggtttat atcgacctga aacgcgtttg
     8221 ccctgaactc gatggactga atatcggcgg tggttggcca tatagaaatt ctttgggatt
     8281 tgaatacgac tatccatata tggcagagga gatcctcaac cgtatcaaac aaacctgcga
     8341 agaagaaaaa gtagaatgtc cgaatatttt cactgaattc ggaagcttta cagtggcaga
     8401 aagcggcgca ttcattttcc aggtgctcgg tgagaagcgt cagaacgata gcgaacgctg
     8461 gtatatgatc gacaattcac tcatgaccac catgccggat tcctggggca tcaactcgaa
     8521 attcattttg ttgcctgtaa ataaatgggg taatgaagtg caacgtagta atatcggcgg
     8581 acttacctgc gatcagatgg attattataa ttcagaagcg cataccaacg aattatatct
     8641 cccgataaat actatgggtg aaccattgta tatcggattt tttcatacag gtgcatatca
     8701 ggaaagtatc tccggatatg gtggaacaaa acactgcctg attccttcgc caaaacatat
     8761 tcttattagt cgaggtgaag aaggagagct gaaatatgaa gtttttgccg atgagcaaca
     8821 agttgaaagt atgctaactg tgcttggcta cgataaactt gatgagcgcg attaattagt
     8881 ggatagcggt ttgaaaatat attggtcgta acgcaatatg gtgatattac ttttttccat
     8941 ataacgcgtt atacctgcat tatttttgat tcctgtaaat cgaacggggt caagcataaa
     9001 tggcgtagcg ggatttaatt tatcggcaga tgcactaatg tcaacaccat agctattcaa
     9061 catcattgca atgtataaag taaagtcata accgcgacat gcaaaatctg atggccgcgt
     9121 actgtatctt tcaataaatg ccgtttcgaa attgattact ttgggcgaat tcctatcaat
     9181 ataatatgca ttaggatagg tgaaatgtag gttttcataa taatcaatag agattgattc
     9241 aaaagaaagg atatgttgca accccaacat ttctaccggc gtttctcttg aaaattttga
     9301 caagtctcgt atcaatccat taacatatag ctcatcataa gatgatataa atacaaagtt
     9361 ttctaccgat ttcattaaag cagtagcttc agcatagcta gtggcatttg acaatcgcat
     9421 tcccttaccc gtgttggcga tattaaaagc agcagcgaaa tcatcggcca ggtgtttttc
     9481 atttggttta tctgagcgat aaatatttat aatattagca aacgggtgtt gtgtttcaat
     9541 cgatttcaac atagcttcca gttgtgcctc aactgtcgga ttggccatca tgaaatattt
     9601 attggtatct atgaaactat tgttcggaga taaaggtgat acaagataga atttattttg
     9661 ctgagctacc ggagatgccg ccttcagact ttcattaaat atcggtccga atactgcatc
     9721 tgatttcatt atttcaggtt tgctgaaaag tgctgcggta aggctgctgt ctttgtagtt
     9781 attaaatacg tgcacattca acttgatgcc aagactgtca agtgtgtcta aagccattaa
     9841 tgctccttca taaaattcta tggatgccaa aggttgaaag cctgtaatat tttcttctga
     9901 actaagtttt tcaagttcag cagcatcaat actaaatggc aatacaaaag atatatcgta
     9961 tgccggtttt ttctccgatg cgaggttgtc gattaccttg gtcggaataa ttacactatc
    10021 cacttttttt ctgatcaccg tatctgcgtg cacagttgtt tgatcagtat ttgttgggtg
    10081 tgaaaccgtg ctatcatgtt gagcaccatt gaaaatgccg catgatgcaa acagaaatac
    10141 tgtagctaat aacacaatga tgtatttatt attcccactc aatggttgcc ggcggttttg
    10201 aactgatatc atacacaaca cggttgatac ccttaacatg attgataata tcactcgaaa
    10261 tctgtgccaa taatgtatgg ggaagatgac tccagtctgc agtcattcca tctgttgaat
    10321 taacgcatct caaggctaca acattttcgt atgtgcgctc atctcccata acacctacgg
    10381 tctgcaccgg caataaaatt gcacctgcct gccaaacctg atcataccat cctgatgatt
    10441 ttaggtggtt gatgaaaatg gcatcagctt cctgcacaat gcgcactttt tcacgagtga
    10501 tgtcaccaat aatacgaatg gccaatcccg ggccggggaa tgggtggcga tgtaatattt
    10561 cgtgttttaa ttgaagtgta tcaccaacct tgcgcacttc atctttaaat aagaaacgca
    10621 gtggttctat tactttaagg tgtaactttt caggaagtcc gcctacatta tgatgcgatt
    10681 taattgttgc agaaggtcca tgcactgaaa ctgattctat cacatccgga tatatggttc
    10741 cctgcgcaag ccattttacg ttggttagtt ttttagattc acgctcaaac acatcaataa
    10801 acaccttacc tatgatcttg cgtttctttt ccggatcact ttcaccttca agagctgaaa
    10861 gaaattcatc ctctgcatca acacctatca cattcaaacc catatcctgg tagctatgca
    10921 ataccgaatt gaattcattc ttgcgcaata gcccattatt tacgaatatc gatatgagat
    10981 ttttcccaat ggccttattt aataatattg cggcaacact ggaatctaca ccaccactaa
    11041 gtcccaatac tacctggtca ctgccgattg tagtttgcaa atacgcaact gtttcttcaa
    11101 caaacgattc cggtgtccat gtttgatgac atccacaagt tccaaccaca aaattttgca
    11161 gcatcttttt tccctgttcg ctatgtgtta cttcagggtg gaattgtatg caatacatcg
    11221 gattgttaaa tcgtccgggg tcggaggcaa aagcagcaac atctattgaa gctgtttttg
    11281 cggttacatg aaatcctgcc ggcacttgca taacggtatc actgtgactc atccacactt
    11341 ggctttcgtc gtctatgcca taaaataact cgcaattgcg gtcctggatg tgtagaaatg
    11401 ctcggccata ttcacgtttt gatgatggct gcaccactcc tccaaaatgc ttggcaacaa
    11461 actgggcacc atagcaaaca ccgagtatcg gacgtttatc aagtattgct tctatatcga
    11521 atgccggctt ttccggagaa gaaaccgaaa aagggcttcc ggataagatg atacctttaa
    11581 tatcaggggt aatttcagga atcttggtgc aggggacgat ctcgctatac acattgagct
    11641 cccggatcct tctggcgatc agttgagtat actgtgagcc gaagtccaga atcagaattt
    11701 tttcagt
//
LOCUS       DAOQHY010000105        11517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2775_length_11517_cov_6.873844, whole genome shotgun sequence.
ACCESSION   DAOQHY010000105 DAOQHY010000000
VERSION     DAOQHY010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11517
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2775_length_11517_cov_6.873844"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1074)
                     /locus_tag="PLL28_09490"
     CDS             complement(85..1074)
                     /locus_tag="PLL28_09490"
                     /inference="COORDINATES: protein motif:HMM:NF040411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6427 family protein"
                     /protein_id="HNF69596.1"
                     /translation="MLRLFQSSNPFTFILLLVYTGLVFSHNLFFPAPITAASHGVLSD
                     GIWQLLRIDSWPLLAIELLQSILMLTTGILISFMMQKFKLINKTTLMPAAVFVLICSY
                     FPEMRIHIAETLCGLCLIILLYKIFGAYNKQRADMVYFDAAIISAIASLIYFPAVIFF
                     FFSLFGLLRMRSTSFREFIIYLSGWLTVAFLLGTYIFWNGSWEAWFASWEVQMHFISG
                     IGELMNATIIAKLSLIGLVFIVTLIFYFDRLSANLIQIRKYMGVFMLLFLFCLVTACL
                     GIGMDEGSLYLLWVPVTVVISYYFFHFKQLWYSEGMHAALLLGTFLFQYLTFA"
     gene            complement(1114..2616)
                     /locus_tag="PLL28_09495"
     CDS             complement(1114..2616)
                     /locus_tag="PLL28_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69597.1"
                     /translation="MIIEGAMWRSAHVIWLIIIGLMLTGLPSKALAQDISISEPQKIS
                     TELIGYSILGRNKNGDVLIYKKYRFRDEIDILDKNMEFKRRKEFSVKNSDYETVEFYK
                     SGDYLFHFYTYRENKMSYLAVQQYNGDLDKKNEPVLIDSSSQRLGENFSEWKIAKAKT
                     TPYYLVYKYELSNGRIDKLFCRVMDESTRIIQQNDINLPDDQFSPILVKEAVSDDGIP
                     LFLFSNDDFNCKKEKTGVQYLYVFPKGNNSVVYTEISDENYCLDEMNFGLDNAHHSIV
                     SIGFLKEDNKDYMVGYLYSTIDILTGTKGTNFHYIFPQQTLDEIAGLAKEKAISNLPV
                     YQIGTIIPRVDGGALLVAEFYDKTVESYEFTNYDPYVGYRTSTRQVEFYEYDDILLFS
                     IKPDGTPSWNNVIRKKQISREDNGVNSSYGVLTLKDRCIFIFNEDVEQNSNVLQYEMS
                     FTGELDRKSLFNANQQEVQIRPGSAEQIALNEIIIPSIYKKSLRFVRIKF"
     gene            complement(2662..3990)
                     /locus_tag="PLL28_09500"
     CDS             complement(2662..3990)
                     /locus_tag="PLL28_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF69598.1"
                     /translation="MTKEKNDKGFNFGAYAFRQFRRNKPAYISLYILGFLALMALLAP
                     VIANERPLFISYKGHHLYPALSFKKNYILTNDDGTKETIQLDIADWKQMKADKVVWAP
                     IPYAPEKTDMLNAGYKGPHDAQLFKNSDGQIINMPSRFRHVMGTNSLGEDVASGLIHG
                     ARVSLTIGFIAMAIASVLGLILGSMAGYFGDSRLRTSRGQFWMVVLGIIAGWFYGFQT
                     RQFNLQDALSTGTGKLIMQLLLSISIAIAVTVIFALIGRFLGKLPFLNKQVNIPVDSI
                     VSRTIEIFHSMPTFILILTIAAIARPSMTNVMIIIGFTSWTGIARLTRAELLRIRQLE
                     YIQAARALGFGELKIILRHALPNGIAPALVSIAFGIASAVLIESSLSFLGIGVPSDVV
                     TWGSLVNDGRQQYSAWWLVVFPGVAIFLTVTVYNLIGEGLRDALDPKHKK"
     gene            complement(3987..5489)
                     /locus_tag="PLL28_09505"
     CDS             complement(3987..5489)
                     /locus_tag="PLL28_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF69599.1"
                     /translation="MGRYLLKRILFFIPTLLIISLLAFIISLQAPGDPLDRMVSAQEN
                     GGEFQAQSANTQKEKDRWRKKLGLDLPVFYFSFSNAATPDTLYRIYDDNLRNASKRLI
                     NTYGNWEQISDWQKSLNILKNSLFEINALHAAAVDSSFSNADRDTIIEMDRLVSALLL
                     AYDTRLIEIKLHQLQATCSAHNYFSPASTSLAGTLNAWNNVKSQSTPGKKYIPAIHFY
                     GYNQYHRWIFGDGNWLTGKGAVDCKGWLRGDFGYSYETKLPVGNVLAERVRWSLFFTL
                     ISVTLAYLISLPIGIKAAAKKDSLFDRTSSVVLFVLYSMPAFWVATLLLMSFANPLAM
                     DIFPSSGIQPVTGIPEGTSWIEAVRMRLPYLVLPTIAYTYSQLAFLSRITRVSTLEII
                     SQDYIRTARAKGLSEGTVIYKHAFRNALLPIITVFSNIFPLAIGGSVILETIFTIPGM
                     GQQVFHAIVSKDYPVIITVFTLTGVLTLIGYLIADILYAIADPRISYANK"
     gene            complement(5580..7463)
                     /locus_tag="PLL28_09510"
     CDS             complement(5580..7463)
                     /locus_tag="PLL28_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNF69600.1"
                     /translation="MRRHLLPIACLISGIAFLSSCGGNKDSGAEIDVTEISDKIKCIK
                     LDDADSILPEWSKENILINHVVGNPDNFHPTNGAHAARSWILQYTSNFVLRSDIMNMD
                     IAPDLAVAMPEISANKLEYTYTLRKEARWDDGAPILAEDVVFMLKANKCPLTNNPITK
                     VYFDNIKNVITYPNDPYKFTIVMKREYILNISFLTDFPVMERKYHDPENVLAGYTFDQ
                     FNDPNFKADKDANLTAWANNFNDPKWGNDLSLLNGSGPYKITEWSRDQALILERKKDH
                     WTQKLASPTVYDVSLPEKIIFKLVLDPNAQKLEFRSQTVDASVWMATPLVKELMTEAD
                     FNKNYNIEFTDNFSFNYIGLNMRPDGVLRKKFFDDVRVRRAMAYVVPVDEIIQAITFG
                     YAKRQASIVSPLKDEYNKDLTLIPLDIEAAKKLLDEAGWKDTDGDNVRDKVIDGVKTD
                     LRIEFKHQAGQKFVEDVARMIKESAYKAGIDLIVVPVDGITLKEQLGKHDFDMYMSAL
                     AGGSQAEDYTQLWHTSAYTSGGSNYCGFGSAETDALLDSIKYTVDPVARRPLMMRLQQ
                     EIYDQQPYIFLYSAARKNIIHKRFGNQIMTFDRPGVILNNLRLLSLYGPQGGSTLKDD
                     TMH"
     gene            7594..8514
                     /locus_tag="PLL28_09515"
     CDS             7594..8514
                     /locus_tag="PLL28_09515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNF69601.1"
                     /translation="MQHILITGGSGLLGRAITAQLHALGYRVAWMSRRIRPAGNSKDL
                     ALPDKVFLWNPESGHLDPEALSWSDVIIHLAGEGIADKRWTPSRKKIIIESRTKTTEL
                     LVSAMRALPQKKHLVIAASAIGFYGPSEKLLLESDPTGNGFLAYSVNEWEKATANFED
                     VALRAVTFRIGIVLSKNGGAYPQLLQTAMIRVLPFLGNGKQMYPWIHVDDVVDMVLFA
                     ISHPVSGTFNAVSPNPVTQAAIMNTYGDVTSGWYLKIPVPKFIIRLLLGEMADMVLLT
                     QMVSAEKIITAGYKFKFPDINAAIRDLEKK"
     gene            complement(8599..10563)
                     /locus_tag="PLL28_09520"
     CDS             complement(8599..10563)
                     /locus_tag="PLL28_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HNF69602.1"
                     /translation="MQKFITAAFISVLAVTGWMATSNLREASPIEYNGTATISGIVTD
                     DVIGQPIIGAQIALNKTDWFTTDSLGQFTIDKLPQGAYSLLVKAEGYTDQEMSITLTE
                     GENKIVSISMSLATLISADTAITEFEQVYENENLKRKSVMANQESLTFGYATMDAPAI
                     SFRGDVYDEDVDGNYNTESYNAIQETGFKEAISNPLSTFSIDVDNASYSNIRRMINYG
                     QQIPADAVRIEEMVNYFDYTYAQPTNNDPFAVQYELGTCPWNSETSLVMIGIQGKDID
                     YSKTAASNLVFLIDVSGSMQSENKLPLVKSSLTKLIDNLKPTDRVALVVYAGAAGEVL
                     PSTPCSNSSKIKSALNQLEAGGSTAGGAGIQLAYEIATKNFIEGGNNRVILCTDGDFN
                     VGSSSDGEMTKLIEEKRKTGVFITVCGFGMGNYKDSKMESIADNGNGAYYYIDDKKEA
                     EKVFGTDLRGTLFTIAKDVKIQIEFNPVMVESYRLIGYENRILNKEDFDNDAKDAGEL
                     GAGHRVTALYEIKRRTTPSQPTGMQQNTIQEDLKYQSTTVKSTAYSTNELMTLKLRYK
                     APDGNTSKLIEKSLFNQPMAFAATSDNFRFASAVTEFGMILRNSEFKGDADINEVLQL
                     ASGAKGADKYGYRDEFIQLVKQYAGTTAKN"
     gene            complement(10706..11062)
                     /locus_tag="PLL28_09525"
     CDS             complement(10706..11062)
                     /locus_tag="PLL28_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69603.1"
                     /translation="MKQRNTILTMLLIGSLALSSCYSTEVPETNKVTYIVTGTSFDVI
                     YQDPAGEYQYVYDREGSFKVYQESLVGESVFLLAKGSDDVVVHIRIKDGIDTVRKAKT
                     AGTSLVYIDYLPEIKE"
     gene            complement(11275..>11517)
                     /locus_tag="PLL28_09530"
     CDS             complement(11275..>11517)
                     /locus_tag="PLL28_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNF69604.1"
                     /translation="RNAFYDELSAAYARCASADELRTILGRGRAKKGMFEGDMEAGEL
                     EIGQVSATVREILPAAEILKDIWEGYLVAKSGLTKQ"
BASE COUNT         3376 a         2465 c         2219 g         3457 t
ORIGIN      
        1 tacattgcgt aatacgtgaa tgatatcctg atcgcaatta gatccgggaa aggttacaac
       61 gccaaatttc atgattcctg attgttatgc aaaggtaagg tactgaaaga ggaatgtacc
      121 taataaaaga gcagcatgca tcccttctga ataccataac tgcttaaaat ggaagaaata
      181 atagctgata accaccgtaa ccggaaccca caacagatat aaacttcctt catccatccc
      241 aattccaagg caggcagtta ccaggcaaaa taaaaacaac aacatgaata cccccatata
      301 cttgcggatc tggatcaggt ttgccgatag gcggtcgaaa taaaaaatta gggtgacgat
      361 gaaaaccaac cctatcaaac tcaacttagc tattatggtt gcattcataa gttctccaat
      421 gcctgaaata aagtgcattt gtacctccca ggaggcaaac caggcttccc aactgccatt
      481 ccagaaaata tatgttccca ataaaaatgc aaccgttagc catcctgata agtaaatgat
      541 aaattctctg aatgaggtcg agcgcattcg cagcaatcca aaaagactga aaaaaaagaa
      601 aattacagca gggaaataga tcaacgaagc aattgcactg atgatggcgg catcgaaata
      661 caccatatca gctctctgtt tgttgtaggc gccaaagatc ttatatagta aaatgatcaa
      721 acacaatcca cacagtgttt ctgcaatatg tatgcgcatc tcagggaaat agctgcatat
      781 caacacaaaa acagcagcgg gcatcaaggt ggttttattg atgagcttga atttctgcat
      841 cataaaactg attaatatcc ctgtggtaag catcaatatc gattgtaata attcaatggc
      901 aagcaatggc catgaatcaa tccgtaacaa ctgccatatc ccatcactca acaccccatg
      961 cgaagctgca gtaataggcg cagggaaaaa taaattgtgt gaaaacacca aacccgtata
     1021 tacgaggagg agtataaagg taaatggatt tgacgattga aataaacgca gcacgatgta
     1081 aaattacact tccaaattaa acattactgc atcctagaat ttgatgcgca caaatctcag
     1141 acttttctta taaatactgg ggataataat ctcattcaaa gcaatctgct cggcacttcc
     1201 cggccttatc tgaacctcct gttgattggc gttgaacaag ctctttcggt ctagctcacc
     1261 ggtgaatgac atctcgtatt gcaatacatt cgaattttgt tcaacatctt cattgaatat
     1321 gaaaatacac ctgtctttca gtgttaaaac cccataggat gaattcacgc cattgtcttc
     1381 tctgctgatt tgttttttac gaatcacatt attccaggaa ggagtgccat caggctttat
     1441 ggagaacaat aaaatatcgt catattcata aaattcgacc tgacgtgtgg atgtcctgta
     1501 tccaacataa gggtcgtagt tggtgaactc ataactttca acggttttat cgtaaaattc
     1561 agcaaccagc aatgcgcctc cgtcaacacg aggaataatg gtgccgattt gatacaccgg
     1621 taagttgctg attgcttttt cctttgctaa gcccgcaatc tcatcaagtg tttgttgagg
     1681 aaatatgtaa tgaaaatttg ttccttttgt gccggttagt atatcaatgg tggaatataa
     1741 atatccaacc atataatctt tattatcttc tttaagaaac cctatgctca caatgctgtg
     1801 atgagcatta tcaagcccga aattcatttc atccaaacag taattttcgt cagagatctc
     1861 tgtataaaca acgctgttat ttccttttgg gaatacgtat aaatattgaa ctcctgtttt
     1921 ctctttcttg caattgaagt catcattcga aaatagaaat aaaggtatcc cgtcatcgct
     1981 aactgcttct tttactaaaa tagggctgaa ttgatcatca ggcaggttga tgtcattttg
     2041 ctgtatgatg cgtgtgcttt cgtccattac acggcagaac agtttgtcta tgcggccatt
     2101 gctcaattca tatttataca ccagataata gggggttgtt ttggccttgg caattttcca
     2161 ttccgagaaa ttttcaccta aacgctgtga agaggagtcg atcaatacag gttcattttt
     2221 tttatctaag tcgccgttat attgttgaac agcgagataa ctcattttat tctcgcgata
     2281 agtgtaaaaa tgaaagagat agtcgcccga tttatagaac tcaacggtct cgtaatcaga
     2341 atttttaaca ctgaattcct tccgacgctt gaactccata ttcttgtcca aaatatctat
     2401 ttcatccctg aaacgatatt tcttataaat aagcacatcg ccattcttgt tgcgacctaa
     2461 aatactgtaa ccaattaact cggtgctgat tttttgcggt tccgatatag agatgtcttg
     2521 tgccaatgct ttagacggca atcccgtaag cattaaacca atgataatca gccatattac
     2581 atgtgcactc cgccacatag caccttcaat aatcatcaaa aataaagtaa gctgattaaa
     2641 acaacggcag tgtagccggg attatttttt atgtttcgga tccagagcat cgcgaagtcc
     2701 ttcaccaatg agattataca ccgttacggt aaggaatata gcaactcccg ggaacacaac
     2761 caaccaccag gcgctatact gttgcctccc atcatttacc agacttcccc aggttaccac
     2821 atccgaggga acgccaatac ctaaaaatga caaacttgat tcaattaata cagccgatgc
     2881 tatgccgaag gcaattgaaa ccagtgccgg ggcaatgcca tttggaagcg catggcgcaa
     2941 tatgatcttt aattcaccga atcccagtgc ccgtgctgcc tgaatatatt ctaattgccg
     3001 tatccgcaac aattcagcgc gtgttaacct ggctatacct gtccatgaag tgaatccgat
     3061 aatgatcatc acatttgtca tcgagggacg tgcaattgcc gcaatagtta aaattaaaat
     3121 aaacgttggc atagagtgaa atatctctat cgttcgtgat acaatggagt caacaggaat
     3181 gtttacctgc ttgttgagga aaggtaattt ccccaaaaat cgtccaatca atgcaaatat
     3241 tactgtcaca gcaattgcaa tactaatgct aagcagtagt tgcatgatta attttcctgt
     3301 tcctgtacta agggcatcct gtaaattaaa ttgtcgcgtt tgaaatccgt aaaaccaacc
     3361 tgctattatt cctaatacga ccatccagaa ttggcctctt gaagtgcgca atctgctatc
     3421 gccgaaatag cctgccattg agcctaatat caacccgaga actgacgcaa tggccattgc
     3481 aataaagcca atagtcagcg atacccttgc accgtgtatc aaacctgacg ccacatcttc
     3541 ccctaaacta ttcgttccca ttacatgcct gaatcttgat ggcatattaa tgatctgacc
     3601 gtccgaattt ttaaataatt gcgcatcatg cggaccttta tacccggcat taagcatgtc
     3661 tgttttctca ggtgcataag gaatgggtgc ccatacaacc ttatccgcct tcatttgttt
     3721 ccagtcggct atatccagtt gaatggtttc cttcgtgcca tcatcattag tcaggatata
     3781 attttttttg aaacttaacg ccggatataa atgatgtcct ttatagctga taaataatgg
     3841 acgttcgttg gcgataaccg gcgccagcaa tgccattaaa gcaagaaacc caagtatata
     3901 taatgatata tatgcaggct tattgcgcct gaattgacga aacgcatatg cgccgaaatt
     3961 aaatccctta tcgtttttct ctttcgtcat ttattagcat atgaaattcg cggatcggca
     4021 atggcgtata aaatatcggc aatcagataa cctattagtg ttaatacacc tgttaatgta
     4081 aatacagtta tgattaccgg ataatctttc gacacaatag catgaaacac ctgctggccc
     4141 ataccgggaa tggtaaatat ggtttccagt attacagacc caccgatggc aagtggaaaa
     4201 atgttactga acaccgtaat aatcggcaat aatgcatttc tgaatgcatg cttgtatatc
     4261 acagtgcctt ccgataatcc tttggctctg gcggtgcgga tataatcctg actgattatt
     4321 tccaaagtcg acactcttgt aatgcgactt aagaacgcaa gttgagaata agtgtatgca
     4381 atggtcggta atacgagata tggcaatcgc atacgcacag cctctatcca tgaggtgcct
     4441 tcggggattc cggttaccgg ttgaatgcca ctcgagggga aaatatccat cgccaatgga
     4501 ttggcaaacg acatcaataa taaagtagct acccaaaacg ccggcatact atataaaaca
     4561 aataaaacta ccgatgaagt tctgtcaaac agggagtctt ttttcgctgc agctttaatt
     4621 ccgataggta agctgatcaa atacgcaagt gttactgaaa ttaaagtaaa aaacaacgac
     4681 caccttacgc gttctgccaa cacatttcct acgggcaatt tagtttcgta actatatcca
     4741 aaatctccgc gaagccatcc cttacaatca acagcacctt ttcccgtaag ccaattgcca
     4801 tcgccaaaaa tccatctgtg atattgatta taaccataga aatgaatggc aggtatatat
     4861 ttttttccgg gggtggattg cgactttaca ttattccagg catttagtgt acctgctagt
     4921 gaggttgatg caggagaaaa atagttgtgt gccgaacagg ttgcctgtaa ttgatgtaat
     4981 ttaatttcga tcaaccgcgt gtcgtaagct aataacaatg cactaaccag cctgtccatc
     5041 tcaatgatag tatctctgtc ggcattggaa aaactgctgt ccactgcagc ggcatgaagt
     5101 gcgtttattt caaaaagtga attttttaaa atattcaggc ttttttgcca atcagatatt
     5161 tgttcccaat ttccgtatgt gttgatcaga cgctttgaag cgttgcgcaa attgtcatca
     5221 taaatgcgat aaagtgtatc aggagttgcg gcattactga agctgaaata aaatacagga
     5281 aggtctaatc ctaatttttt ccgccaacgg tctttttctt tttgagtgtt tgccgattgc
     5341 gcctgaaact ctcctccatt ttcctgggca ctaaccattc tgtccagagg atctcccggt
     5401 gcctgcaaac taatgatgaa tgctaaaagt gaaatgatca gcagggtagg aataaaaaat
     5461 aatatgcgct tcaaaagata cctgcccatt gtagattaat tgcacgctaa gataaaaaaa
     5521 gaaaagggtg tgcagcctta attatcgccg cacacccctt gtaaggtagt gaaatgttgt
     5581 taatgcatcg tatcgtcttt cagggtagat cctccctgag ggccatataa actcaataaa
     5641 cgcaagttat ttagtatcac acccgggcga tcaaaggtca tgatttgatt tccgaaacgt
     5701 ttatgtatga tgttttttct ggcagcagaa tacaagaaaa tatagggctg ctggtcatag
     5761 atctcttgtt gaagtcgcat catcagcggc ctgcgggcaa cagggtctac cgtatacttg
     5821 atggaatcaa gcagtgcatc ggtttctgct gaaccaaatc cgcagtaatt cgatccgccg
     5881 gaagtataag cgctggtatg ccataattgt gtataatcct ctgcctgcga tccaccggca
     5941 agagcactca tatacatatc aaaatcatgc ttgcctagct gttcctttag tgtaatgcca
     6001 tcaacaggaa caacaataag atcaataccg gctttatatg ctgattcttt gatcatcctc
     6061 gcaacatctt caacaaattt ttgtcctgcc tgatgtttaa attcaatacg caaatctgtc
     6121 ttcacgccat ctattacctt gtcgcgcaca ttatcgccat cagtatcttt ccatccggct
     6181 tcatcaagta atttttttgc tgcttctatg tcgagcggaa tcaatgtgag gtctttattg
     6241 tattcatctt tcagtggtga tacaatgctt gcctgacgtt tcgcatatcc aaaggttatc
     6301 gcctgtatga tctcgtctac aggtacaaca tatgccattg cacgacgcac gcgcacatca
     6361 tcaaagaatt tttttcgcaa aacaccatcg ggacgcatgt tcaatccgat ataattaaaa
     6421 ctgaaattgt cggtaaattc tatattataa tttttattga aatctgcctc cgtcattaat
     6481 tcttttacca atggtgtagc catccataca gaagcatcaa ctgtttgact ccggaattca
     6541 agtttttgtg cattcgggtc aagtacgagt ttgaaaatga ttttttcggg aagtgataca
     6601 tcatatactg ttggtgatgc taatttttgc gtccaatggt cttttttccg ctcgaggatc
     6661 aaagcctgat cgcgcgacca ttcagtaatt ttgtaaggtc ctgaaccatt taacagagaa
     6721 agatcattac cccatttcgg atcattgaaa ttatttgccc acgctgtaag gttcgcatcc
     6781 ttgtcggctt tgaaattcgg atcattgaat tggtcgaatg tataaccagc aagcacattc
     6841 tcaggatcgt gatacttgcg ctccatcacc gggaaatcgg taagaaaaga tatatttaaa
     6901 atatattctc tcttcatcac gatggtgaac ttatagggat cgttcggata agtgatcaca
     6961 tttttaatat tgtcgaaata aacttttgtg atcggattat ttgtaagcgg acatttattt
     7021 gccttcaaca taaaaacaac atcctctgct aagatcggag caccatcatc ccatcgcgct
     7081 tctttacgta aggtgtaagt gtattcgagt ttgttggcag atatttcagg catggcaact
     7141 gccagatccg gagcaatatc catattcata atgtcgctgc gcaatacaaa attgctggtg
     7201 tattgaagta tccagcttct tgctgcatga gcgccatttg tcggatggaa attgtcggga
     7261 ttgcctacaa catgattgat aagaatattt tctttgctcc attccggtaa aatggaatca
     7321 gcatcatcta atttgatgca cttgatttta tccgatattt ctgtaacatc aatttcggca
     7381 ccggagtcct tatttcctcc acaagaactc aaaaatgcaa tgcctgatat aagacaggca
     7441 attggcagta aatgtctacg catgatagat tgttaattac gacccgaaag taagaatttt
     7501 atgcgattcg gcgatttcct gatatctgat tttggaacga aaattgctgc aaaaggtaaa
     7561 ccaattcggc actctactgt ttaactccac taaatgcaac acattctcat caccggcggt
     7621 agcgggctac taggaagggc aattacagca caattgcatg cattgggcta tcgcgttgcg
     7681 tggatgagta ggaggataag acctgcaggc aactcgaaag accttgcgct gccggataaa
     7741 gttttcctgt ggaacccgga atccgggcat ctcgacccgg aggcgctatc atggagtgat
     7801 gtcatcatcc acttggcagg agagggtatt gcagataagc gatggacacc ctccagaaaa
     7861 aaaatcatca ttgaaagccg cacaaaaaca acagaactgt tggtgtcggc catgcgcgcg
     7921 ttgcctcaga aaaaacatct ggttattgcc gcttcagcca tcggttttta cggaccatcg
     7981 gaaaagctgc ttctcgaatc tgatcctacc ggcaatggat ttttggcata cagcgtgaat
     8041 gaatgggaaa aggctacggc aaattttgag gatgttgcct taagagcggt gacgtttaga
     8101 attggtatcg tattgtcgaa gaacggtggt gcctacccgc agttgttgca aacagcaatg
     8161 ataagggtgt tgcctttttt gggtaatgga aaacaaatgt acccttggat acatgtggat
     8221 gatgtcgtgg acatggtgct ttttgccata agtcatcctg tttcaggaac cttcaacgca
     8281 gtatctccaa atcctgtcac ccaggcggca atcatgaata cgtatggtga tgtaacatcc
     8341 ggatggtatc ttaaaattcc tgttccgaaa tttattattc gattattgtt gggagaaatg
     8401 gctgatatgg tactgctcac acaaatggtg tcggcagaaa aaataataac agcaggttac
     8461 aaatttaaat ttccggatat caatgcagct atccgtgacc ttgaaaaaaa ataattccgg
     8521 aatacagctt tcgccatacc ccggaattgc atcaaacatc aagatttatt aaaagacgcc
     8581 ctaacttttt ttcgaatttt aatttttggc agttgttcct gcatattgtt ttaccagttg
     8641 aataaattcg tcgcgatagc cgtatttatc tgcgccctta gcgccacttg ccaattgcaa
     8701 aacttcattt atatcagcat cgcctttgaa ttctgaattt ctcagtatca taccaaattc
     8761 tgttactgcg cttgcaaacc tgaaattatc gctggtagct gcaaatgcca tgggttgatt
     8821 gaaaagtgat ttttcaatga gtttgctggt atttccatca ggggctttat acctcagctt
     8881 caatgtcatt aattcattgg tagaataggc agtggattta acagttgtcg attgatattt
     8941 caaatcttcc tgtatggtgt tttgttgcat tcctgttggt tgtgatggcg ttgtgcgacg
     9001 tttgatttca tacagtgctg taacgcgatg ccctgcaccc aattcaccgg catcttttgc
     9061 atcgttgtcg aaatcttctt tattcaaaat cctgttttca taacctataa ggcgataact
     9121 ttctaccata accggattga attctatttg aatttttaca tctttagcta tggtaaataa
     9181 cgtacctcga agatctgttc caaaaacttt ttcagcttct tttttgtcgt cgatgtaata
     9241 atatgcacca ttgccattat ctgctatcga ttccatttta ctgtctttat aatttcccat
     9301 tccaaagccg caaacggtaa taaatacacc tgtttttctt ttttcctcga taagcttagt
     9361 catctctcca tcgctgctgg agcctacatt gaaatcgccg tcggtgcaaa gtatcactcg
     9421 gttgttgcca ccttcaataa aattttttgt cgcaatttca tatgccagct gaatacctgc
     9481 tccgcctgca gtgctgcctc ctgcttctaa ttgattgagg gcgcttttga ttttagaact
     9541 attgctgcag ggtgtcgatg gcaatacctc acccgctgca ccggcatata caaccaaggc
     9601 tactcgatct gttggtttta gattgtcaat cagcttggtt aaactgcttt ttactaaggg
     9661 caatttattt tcgctctgca tgctaccact tacatcgatc agaaatacaa ggtttgaagc
     9721 ggcagtttta gaataatcga tgtctttgcc ttgtatgcct atcatcacca gcgatgtttc
     9781 cgaattccag gggcatgttc ccaattcata ttgcactgca aacgggtcgt tgtttgtagg
     9841 ttgtgcatag gtgtagtcaa aataatttac catttcttca atgcgcacag catcagccgg
     9901 aatttgttgc ccataattaa tcatcctgcg aatgtttgaa tacgacgcat tatctacatc
     9961 aatagaaaat gttgaaagtg gattgctgat agcctcctta aaacctgttt cttgtatggc
    10021 attgtaagat tctgtgttgt aatttccgtc aacatcttca tcatatacat cacctctgaa
    10081 tgaaattgcc ggagcatcca ttgtggcata accaaatgtc agagactcct gattggccat
    10141 cacacttttt ctttttaaat tttcattttc ataaacctgt tcaaactcgg taatggctgt
    10201 atcagcactt atcaatgttg caagagacat gctgatactg acaattttat tttcgccttc
    10261 agtaagtgtt atcgacattt cctggtcggt ataaccttca gcttttacca acaaactata
    10321 tgccccttga ggaagtttgt caatagtaaa ttgtccaagt gaatcagtag taaaccaatc
    10381 cgttttattc aatgcgatct gtgcgccaat aattggttgc ccgataacat catcggtcac
    10441 tatgcctgaa attgtagctg tgccattata ttcaatcggc gatgcttcgc gaaggtttga
    10501 ggttgccatc catcctgtaa ctgcaagaac agatataaag gccgcggtga tgaatttttg
    10561 catgatagca gatttttaaa gtgaagtaat aactgctatc ctgatgcatg aaaattctct
    10621 tttccataaa agccgtcaat aatcgttggg acaaaaaaaa atcccggtcg caaaaccggg
    10681 acgttcaaat aaggtgtgaa agcgtttatt cttttatttc aggcaaatag tcaatataga
    10741 ccaatgaagt ccctgccgtt tttgcctttc taactgtgtc aatgccatct ttaatacgga
    10801 tatgcactac cacatcatca ctgccctttg caagcagaaa taccgactcg ccaactaaag
    10861 attcctgata aactttaaag gatccttctc ggtcgtaaac atattggtac tctcccgcag
    10921 ggtcttgata gataacatca aaagatgttc ctgttacaat ataggtgacc ttattggttt
    10981 cgggcacctc ggtactgtag cacgacgaaa gtgccagaga accaatcagt aacattgtta
    11041 aaattgtgtt gcgttgcttc atactcgtga tacttgaaat actaagatag acatttccac
    11101 agggcgtatg tatatacagt aaatgttatt gtgtgacatg gtaaaacgag gcaaaagtat
    11161 gaaaatatcg tctagagcac cttattgctg acaaaaaaat gtataaaaaa cggtttaaat
    11221 gtgacatcga tatcgtcact attgaagtta ggttaccttg cttttgggca tggttcattg
    11281 cttagtcagg cctgattttg ccaccaaata accctcccaa atgtccttca ggatttcagc
    11341 tgcgggcaat atttctcgta ccgtagcgct aacctgtcct atttctagtt cacctgcttc
    11401 catatcgccc tcaaacatac cttttttagc cctgccgcgc cctagaattg tcctgagttc
    11461 gtcggcactt gcacaacggg cataagctgc tgaaagctcg tcatagaaag catttcg
//
LOCUS       DAOQHY010000106        11417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2817_length_11417_cov_4.927566, whole genome shotgun sequence.
ACCESSION   DAOQHY010000106 DAOQHY010000000
VERSION     DAOQHY010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11417
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2817_length_11417_cov_4.927566"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="PLL28_09535"
     CDS             <1..745
                     /locus_tag="PLL28_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69605.1"
                     /translation="ALSESKPVKLSGIGRLTMDVEGQIRFLPDNTQNFQLQSYGLYSF
                     TAQPITRDRQQIEVQTAKPVQAIEVPARKKRKFSDALLPIAIVLLICMGAMQVFIQKD
                     MNGFNYSEIFGLSKLFPHNDYALKRYETDVYQINSSITYFRRTMPAPADTTVADSSSL
                     TTSPAAVNTQPVSTHATYFVSAGSFYSSSQAQKVVDKIAAKGYTGYVKPYGKYQVAAV
                     DIPDNISQSAFRKAFIEATGIKGAWIGKN"
     gene            complement(914..1645)
                     /locus_tag="PLL28_09540"
     CDS             complement(914..1645)
                     /locus_tag="PLL28_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69606.1"
                     /translation="MALLLTTVWKEHLPSGIVNFIYVTLPLQALPENSGTIFTIDYSN
                     QTIYNKMKKVSLLLVMATTLLFTNSFAQVTQKGGTSTDKPATTAEKPSSDKKDKVQSE
                     MSGDAASTAKEWTMKMDEICNLDQAQEKKVMEINLRYANKLEELKAKYKGMENPNADA
                     AKAEKDELTKARFKEYSNVLSKEQMQKFKEYRNSKKDENKGDSAGKSEKKDDMKEKYK
                     NATPEEKEKMKEEMKDKKDKKQNKE"
     gene            complement(1602..2555)
                     /locus_tag="PLL28_09545"
     CDS             complement(1602..2555)
                     /locus_tag="PLL28_09545"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNF69607.1"
                     /translation="MTGNDIGFAAQKLREGALVAIPTETVYGLAANAFDKEAVTRIFE
                     AKQRPQFNPLIVHTSDIERVNSFTTGISGVLEQLATTFWPGPLTILLPRNHMIHPLVT
                     AGSDFVAVRIPNHPMTLALLRNLEFPLAAPSANLFGRVSPTTAAHVEEQLGEKVSYIL
                     DGGNCAVGVESTIVKLKNDGVVSILRPGGVSAEMMHPILGYEPEFSTPQGDRPEAPGM
                     LKSHYAPKIPLVLGEAAAMLEKYKQQRVCLITFSEGIAHSSVIRNIILSGRGDLHEAA
                     QQLFSALHEAENEDVEIIIAERVPEIGIGIAINDRLERASA"
     gene            complement(2552..3229)
                     /locus_tag="PLL28_09550"
     CDS             complement(2552..3229)
                     /locus_tag="PLL28_09550"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNF69608.1"
                     /translation="MALKAISPWQRIFSYFKPIQLTTQHSEFTPVLEVVLTEGRCMLN
                     SPDATYSFEDKYTSYRTAIHQIRNRLKNYNHILVLGLGLGSVPYMLQTKFAFKGKITC
                     VEIDPVIVSLAKHYYPNKQLLQKLDLFTDDAVHFMEHNKLQFDAIFCDLFIDKRVPAA
                     LHEFDFLHHVKSALAPGGTLLFSRLASERQAELTLWENLKQVFPEGEDIDTGGNYILN
                     YQAPITT"
     gene            complement(3239..4030)
                     /locus_tag="PLL28_09555"
     CDS             complement(3239..4030)
                     /locus_tag="PLL28_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNF69609.1"
                     /translation="MKKLLWIVIPAMLITACTRVPITNRKQVHLLPESQMISMSLAAY
                     SDFLKESDVVDAGKDNNSVHRVADKLIPAVTKVLNDEGYGDLIKGYQWEVNLVESKEP
                     NAWCMPGGKIVVYTGILPYTKDDAGLAVVLGHEIAHAVARHGNERMSQGLLAETGFMA
                     LDVALANKPTETRNLLMTAVGVGTQVGVLLPFSRTQESEADRLGLIFMAAAGYDPHNA
                     INFWERMSANSAGIPEFLSTHPSDETRITNIRDTYMNEAMKYYSK"
     gene            4146..6410
                     /locus_tag="PLL28_09560"
     CDS             4146..6410
                     /locus_tag="PLL28_09560"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF69610.1"
                     /translation="MKRLTALVSALFITSLSSLLSAQNCTVAGVVTDKSNEEPLIGAT
                     ITLQGASVMRVSTDLDGAFKMMVAEGKYQMIVAYIGYQNDTISITASPGKEIILPIRL
                     EEFAGLLNTVVVTGSKFEKKIGEETVTIDVIKTDLIEHTNDFRLDQTVQRMPGVSVID
                     DQANIRGGAGYSYGAGSRVLILMDDLPMLTGDAAFPSWDFIPIENIQQVEIIKGASSA
                     LYGSSALNGIINVRTVFPTSTPETKLSIFNGVYFSPKDTAQKWWGDDMPFTAGGSFAH
                     KQKFNRFDLVTGAYVFNQESYLKEIYNHRARANANMRYRINNNFVLGLNVNAQVNRSA
                     NFFFWNNIDSGLYIPAPNTASFNRGFKITVDPYLNYYDAKGNRHKILTRYYGNHNVTP
                     GIDQSNFNDLFYGEYQYQKHLEDIRLVITSGVIGSKTYSNADLYGDSSYTSGNEAAYL
                     QFDKKLGNKWNLSAGMRYEFNQIGELKESRPVFRAGANYQIAKYTFARASWGQGYRFP
                     TIAEKYISTGISGLSIFPNPDLTSETGWSAEAGIKQGVKFSNWNGLMDISGFMSEYYD
                     MMEFTFGYYPTPGFMSLNIGDTRIMGAEISFIGEGKIGNIPTTLIAGYTYIDPKFQVF
                     DSLQNALSSADYNVLKYRFRHTVKFDVESTFHKFRFGAACNYTSFMEAVDAAFVDPII
                     PAIGLYIIEGLQEYRDEHDSGDYVIDLRAAWVFNDRNEISVNCNNLLNREYSVRPAKM
                     EAPRNIMVKYSLNL"
     gene            6465..6752
                     /locus_tag="PLL28_09565"
     CDS             6465..6752
                     /locus_tag="PLL28_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69611.1"
                     /translation="MTLGALTDKLRKRIVEFRWKHRFETKVSPAINFIEMALNITELA
                     MVKSRPIDILEEHWFHEGWPVIHTLEDTEWEDIIDLYRTLTFKVQERNWFR"
     gene            complement(6753..7766)
                     /locus_tag="PLL28_09570"
     CDS             complement(6753..7766)
                     /locus_tag="PLL28_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69612.1"
                     /translation="MKDTLPQENPYQLDFIGRLKGMLPAHISLADQLIDILNISQDSA
                     YRRIRGETAMSIDEAITLCNHYKIPIAYFTDEIKGLVNFRYVAPDGTKASFIKHIEMI
                     YKSSDELLNHEDRRIQYSAIDTPLFLQFAFEPLAKFKMHFWMREIEGIDDFKPHYDPN
                     DIDRELVTLANKIHDVYLMIPSDEIWPDDIMHTTLKQLHYYHEADYFKKKEDLEEIIA
                     QLNHLIDHTEAMASISRKLKYGDRLKQPYQEFNLYQSDVLLGNNTVLTFGGGKTTSYI
                     SHMTFSILSTNSARYASETEAWMKSILNKSNLISGISEKNRSKYFRHLRKVVEIAREK
                     MLA"
     regulatory      8070..8275
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8362..9645
                     /locus_tag="PLL28_09575"
     CDS             8362..9645
                     /locus_tag="PLL28_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69613.1"
                     /translation="MKKFMYLILAVMITTSAFSQAYVSRVLVLNEGYFDYITNDIIVP
                     VTVGAYDPQTDTYTTIQTIDEARFASDIAIDGDYYYVAADHFLQKYDLLTNALISSID
                     VEGIRQIAITNDEIIVTRGEYLVTLPSYIQVYQKETMTLDYEIPADVIPHTTEGVIVK
                     DGKAYIAVNNGFVFGGEVGYIAVLDLATAYVENIIDLGADGINPDNLMIEGDQLFTLN
                     NKDFTGSSVSSYKIGTGELATTNLANISSGCGTSAISGGSIYYQELFGTTISKFDPAT
                     ETITDENEYGISFYGLSFDPLNGQMYTSETDYFSYGKVYVYNTDGTLSTSFDAGVSPG
                     NFAFDVRMESGVKDLKSDFLKMQPNPATEQISLKADFNIETVSISNIQGASCLTLDGS
                     HADLMLIDITKLPAGQYILSATGDSSTQSAMFIKQ"
     gene            9695..10810
                     /locus_tag="PLL28_09580"
     CDS             9695..10810
                     /locus_tag="PLL28_09580"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNF69614.1"
                     /translation="MRLSLVAILICLLTTGCRPTDSTPELPHGKRVAVQFARGFIIEQ
                     IGDSYLVTVRDPLDTTHILATYRLKHDNKKGKDQINIPVSRLVSFSTTHIGFLDVLGV
                     QNTLVGFSGTTYICNDSVRKMVDEGKIVEVGNEGGIDHEKIVMLHPDIVMTYLTGNAA
                     YDQVDKLYTLGLQPVINNEFMELSPLGQAEWIKFIAVFYDRLDEANAIFDKVMTEYIA
                     TSSLVGERGDRPTVFTGMAYKGEWTIPGGKSFAAGYIHDAGGKYLWESDSKTGNFPVS
                     FETMLSIAGEAEIWLHPGASASSAEILAADERYASFDALKTGHIFNNNARLCEGGGND
                     YWERGIVFPNEVLADLVAIFHPEVLKDHTFTFYTHLR"
     gene            10852..>11417
                     /locus_tag="PLL28_09585"
     CDS             10852..>11417
                     /locus_tag="PLL28_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HNF69615.1"
                     /translation="MTILLFFADLLVGSTPITISQLFANLLGKQDNDDINTIISLFRL
                     PKAYTALLVGAALPVAGLLMQTFFRNPVAGPDILGVTAGSSLFVAIVMMTGGLFFGID
                     AFYGMTVVLSAIAGAVVLLLIILLVAARLRDTMTLLIFGIMFGMAISAIVSIIQYYSE
                     RDALRLFMLWTFGSLGGVTHAQLIIMVP"
BASE COUNT         3201 a         2295 c         2470 g         3451 t
ORIGIN      
        1 agcactttca gagtcaaaac cggttaaatt atccggaatt ggcagactga caatggatgt
       61 tgaaggccag atccgcttct tacccgacaa tacgcagaat tttcaactgc agagctatgg
      121 gttatactca tttactgccc agccgattac acgcgacagg cagcaaatag aagtgcaaac
      181 cgccaaacct gttcaggcta ttgaagttcc ggcccggaaa aaacgcaagt tttctgatgc
      241 cctcttacct attgcaatag tgcttttaat ttgcatgggg gcaatgcagg tttttattca
      301 gaaggatatg aatggtttca actattcgga aatttttgga ctaagcaaac tgtttcccca
      361 caatgactat gcgttgaagc gatacgaaac agatgtttat caaattaact catcaatcac
      421 ctacttccgc agaaccatgc ccgctccggc agatacaact gtggcagatt catcaagttt
      481 aactacttca cctgcagcgg taaacacaca gcccgtatcc actcatgcaa cgtattttgt
      541 atcagcaggc tcattctata gctcgtcgca ggcacaaaag gtggtcgaca agattgcagc
      601 gaaaggctat acaggttatg taaaacctta tggcaaatat caggtggcag cagttgatat
      661 ccctgataat atctctcaat cagcgtttcg caaggccttt atagaggcaa caggcattaa
      721 gggcgcctgg ataggtaaga actaaaattg cattgcttaa ttggccttga attggatatc
      781 catcgctttt aaaaggcgag aaattggctt ttaataacat cttgaatata cctgcaattg
      841 tgcctggaca tctggagtaa tgtacataaa aaaagccccc gtttaggagg ctttcttatc
      901 tttcagaagg atattattct ttgttttgtt ttttatcctt cttgtctttc atctcttctt
      961 tcattttctc tttttcttca ggagtagcat ttttatactt ctctttcatg tcatctttct
     1021 tctcagattt acctgcgctg tcgcctttgt tttcatcttt tttgctattg cggtattctt
     1081 tgaatttctg catttgctct tttgaaagaa cattgctgta ttctttgaaa cgcgctttag
     1141 tcaattcatc tttttcagct ttggcagcat cagcattcgg attttccatg cctttgtatt
     1201 ttgctttcag ctcttcaagt ttgttggcat agcgaaggtt gatttccata acttttttct
     1261 cctgggcctg atccagatta cagatttcat ccattttcat ggtccattct tttgcagtag
     1321 aagctgcatc gccggacatt tcactctgca ctttgtcttt cttgtcagaa gatggttttt
     1381 ctgcagttgt agcaggttta tcagtgctag tgccaccttt ttgagtcacc tgggcgaaac
     1441 tgttagtaaa cagtaaggtg gttgccatca ccaggagtaa acttaccttt ttcattttgt
     1501 tgtaaattgt ttggtttgaa taatctattg taaatatcgt gccagaattt tcagggaggg
     1561 cttggagtgg caaagttaca tatataaaat taacaatacc actaggcaga tgctctttcc
     1621 agacggtcgt taatagcaat gccaatgccg atttcaggca cacgctcggc gataattatt
     1681 tctacatctt cattttcagc ctcatgcaaa gctgagaaca attgttgtgc agcctcatgt
     1741 aagtcgcctc taccactcaa aatgatgttt cggataacac tcgaatgcgc tatcccttcg
     1801 gaaaaggtga ttaagcaaac gcgctgttgc ttgtattttt ctagcatagc agcagcttcc
     1861 cccaaaacca gtggaatctt tggcgcataa tgactcttca gcattccggg cgcttcaggg
     1921 cgatcacctt gtggtgtgct gaattccggt tcatacccta aaataggatg catcatttcg
     1981 gcagaaacac caccgggacg aaggatgctt acaaccccat catttttcag cttaacgatg
     2041 gtgctttcaa cccctaccgc acaatttcca ccatccaaaa tataggatac cttctcgcct
     2101 aattgctctt caacatgcgc tgcagttgtg ggactaaccc tgccaaaaag attagcactc
     2161 ggcgctgcca gagggaattc caggttgcgt agcaaggcta aagtcattgg gtgatttggt
     2221 attctgacag ccacaaagtc acttcccgct gttacaagcg gatgtatcat gtgattcctc
     2281 ggtagcagga tcgtcaatgg tcccggccaa aaggttgttg ccaattgctc caaaacgcct
     2341 gaaatgcccg ttgtgaaaga gtttacccgc tcaatatctg aggtgtgaac gatcaacgga
     2401 ttgaattggg gtctttgttt agcttcgaaa atacgtgtaa cggcctcctt gtcgaacgcg
     2461 ttggctgcca gtccgtaaac tgtttccgta ggaatggcta ccagagcgcc ttcccgcaac
     2521 ttctgcgccg caaaaccaat gtcatttccc gtcatgttgt aattggtgcc tggtaattta
     2581 aaatataatt cccgccggta tctatatcct caccttcggg aaaaacttgc ttgagatttt
     2641 cccacaaagt taattcagcc tgccgctctg atgccaaccg gctgaataaa agtgtgccac
     2701 ccggcgcaag cgcgcttttt acatgatgca ggaagtcgaa ctcatgcaag gctgcaggga
     2761 cacgtttatc aataaacaaa tcgcagaaaa tggcatcgaa ttgcagcttg ttatgctcca
     2821 taaagtgaac cgcatcatcg gtaaacaaat cgagcttctg taatagctgc ttgtttggat
     2881 aataatgttt tgccagagat acaattacag gatcaatctc tacacatgtg atcttgccct
     2941 tgaaggcaaa tttggtttgt agcatataag gcacagatcc taagcccaat cccaatacta
     3001 atatgtgatt gtaattttta agcctgttgc gaatctgatg aatggccgtt ctgtagctag
     3061 tgtatttatc ttcaaatgaa taagtagcat ccggagaatt gagcatacag cgtccttctg
     3121 taagcacgac ctccaaaaca ggtgtaaatt cactgtgttg agttgtgagc tgtataggct
     3181 tgaaatagct aaaaatgcgt tgccatggcg atattgcctt tagtgccatg gtgcaaagct
     3241 acttcgaata atacttcata gcctcattca tatacgtatc gcggatattg gtgatcctgg
     3301 tttcgtcgct tggatgcgta ctcaaaaact ccggaatccc tgcactgtta gcactcatgc
     3361 gttcccaaaa gttgatggca ttgtgcggat catacccagc ggcagccata aatataagtc
     3421 ccaatcgatc ggcttcagac tcttgtgtgc ggctgaaagg cagcaaaaca ccaacttgtg
     3481 tacctacacc tacggcagtc ataagcaaat ttcttgtttc tgttggcttg ttggctaaag
     3541 caacatcaag tgccataaat ccggtttcag cgagtaaacc ctggctcata cgttcatttc
     3601 cgtggcgcgc aacggcatgg gctatctcgt gtcccaatac tactgccaag ccggcgtcat
     3661 ccttggtgta gggtagaatt ccggtataca caacaatctt tccgccgggc atgcaccagg
     3721 cattgggttc tttactctca accaggttta cttcccattg gtaacctttt atcaggtcgc
     3781 catagccctc atcattcaac actttggtaa cggcaggtat cagcttatct gccacgcggt
     3841 gaacggaatt gttatccttg cctgcgtcta ccacatcact ttccttgagg aaatcgctgt
     3901 atgctgcgag actcatcgag atcatctgac tttcaggcag taaatgcacc tgtttgcggt
     3961 tggtaatagg cacgcgtgtg caggcggtga tcaacatagc ggggattaca atccaaagca
     4021 gcttcttcat ctgtataggt tttgggttag tcatgcaaat atcgtactca atttgtaaat
     4081 cgtgtgcaga actctgtttc ctgaaaggta ggtttattta tctttacccg tcaacaaaac
     4141 cacacatgaa gcgactgaca gccttagttt cagccctgtt tataacatct ctttcttcgt
     4201 tattatcggc ccaaaattgt actgttgccg gagtcgtaac tgacaagtct aatgaagagc
     4261 cgctcatagg agctactata acacttcaag gtgcaagtgt tatgcgggtg agcaccgatc
     4321 ttgatggagc ctttaaaatg atggttgctg aaggtaaata ccaaatgata gttgcttata
     4381 tcggttatca aaatgatact atttcaatta ccgcaagtcc cggaaaagaa attatacttc
     4441 ctattcgact ggaggaattc gcaggattgc tgaatactgt ggtggtgaca ggttctaaat
     4501 tcgagaaaaa aataggagag gaaacagtta ccattgatgt cataaaaaca gatttgattg
     4561 aacacacaaa tgattttcgt ttggaccaaa cagttcagcg gatgccggga gttagtgtga
     4621 ttgatgatca ggcaaatatc cgtggcggag caggatatag ctatggtgca ggaagcaggg
     4681 tgctcattct gatggatgac ctgcccatgt tgaccggtga tgcggcattt ccgagctggg
     4741 attttattcc catagaaaat atccaacaag tagagatcat caaaggcgct tcaagtgcat
     4801 tgtatggttc cagtgccttg aatggtatca ttaatgttcg cacagtattt cccacatcaa
     4861 cacccgaaac caaactttcc atttttaatg gcgtttattt cagtccgaaa gataccgcac
     4921 agaaatggtg gggtgatgat atgccgttta ctgcaggagg gagttttgcg cacaaacaaa
     4981 aattcaaccg atttgatctt gtaaccggag cgtatgtatt taatcaagaa agttatttga
     5041 aagaaatcta caaccacagg gcaagagcaa atgcgaatat gcgctatcgc attaataata
     5101 attttgtgct ggggttaaat gtaaatgcac aagtcaatag atcggcgaat ttctttttct
     5161 ggaataatat tgactccggg ttatatattc cggcgcctaa tactgcctct ttcaatcgcg
     5221 ggtttaaaat cactgtagat ccttatctta actattacga tgcaaaaggg aacagacata
     5281 aaatactgac tcgctattac ggcaatcata atgttactcc cggaatcgat caaagtaatt
     5341 tcaatgacct tttttacgga gaatatcaat atcaaaaaca tctggaggat atcagacttg
     5401 tgattacctc gggtgtaatt ggcagtaaaa cttattctaa cgccgatttg tacggcgata
     5461 gttcatatac ctcaggcaat gaagcggcat acctgcaatt cgataaaaag cttggtaata
     5521 aatggaattt aagtgcggga atgcgatatg aatttaatca aatcggagaa ttgaaggaat
     5581 cgagaccggt gttcagagcc ggagcgaatt atcaaattgc aaaatatact ttcgctcgtg
     5641 catcctgggg gcaaggatat cgctttccga caattgctga aaaatatatt tcgaccggta
     5701 tttcaggctt gagtattttc ccaaatcctg atttgacaag cgaaacaggc tggagtgcag
     5761 aagcaggtat taagcaaggt gtcaagtttt ctaactggaa tggattaatg gatatttcag
     5821 ggtttatgtc ggagtactac gacatgatgg aattcacttt cggttattat ccaacaccgg
     5881 gattcatgtc gctgaatatt ggcgatacaa gaataatggg tgcagagatt tcttttatcg
     5941 gtgaagggaa aatcggaaat attcctacta ctttaattgc aggatatacc tatatcgatc
     6001 caaaatttca ggtatttgat tcattgcaaa atgcattatc cagtgccgac tataacgtgc
     6061 tgaaatacag attccgtcat accgtgaaat ttgatgttga gagcaccttc cacaaattca
     6121 gatttggcgc agcatgcaat tataccagtt ttatggaagc cgttgacgcc gcatttgttg
     6181 acccgattat tcctgcaatt ggactgtata tcattgaagg acttcaggaa tacagagatg
     6241 aacatgattc cggcgattat gtgattgacc ttcgcgctgc ctgggtgttt aacgaccgga
     6301 atgaaatcag tgtaaattgt aataatttat taaatcgcga atattccgtt cgccctgcga
     6361 aaatggaagc accaagaaat atcatggtga aatattcact gaatctttaa ttgcgattta
     6421 atactattta ctattagtga attaggatta tagtttacag agccatgacc ttaggagccc
     6481 ttacagataa attgaggaag cgcatcgttg aattcagatg gaagcatcga ttcgaaacaa
     6541 aggtgtcgcc tgcaattaat tttatcgaaa tggcactgaa tatcacagaa ttggctatgg
     6601 tgaaaagtag acctatagat atacttgaag aacactggtt tcatgagggc tggcctgtca
     6661 ttcatacact agaagataca gagtgggaag atattattga tctgtatcgc acccttacct
     6721 tcaaagtgca ggaacgcaat tggtttcgtt aattaggcca gcattttctc gcgagcaatt
     6781 tctactacct ttcgaagatg tctgaaatat tttgagcggt ttttctcaga gatgccactg
     6841 atcagatttg atttattgag aatgctcttc atccaggctt ctgtttcaga ggcatagcgt
     6901 gcgctgtttg tgcttaaaat actgaaagtc atatgactga tataacttgt agtttttcct
     6961 ccgccaaagg ttagtacagt attattgccc aggagcacat cactttgata taaattgaac
     7021 tcctgataag gctgttttaa tctgtcgcca tactttaatt ttctgctaat tgatgccata
     7081 gcttcggtat gatcaatcag gtgattgagt tgagcaataa tttcttccag atcttctttc
     7141 tttttgaaat aatctgcttc atgatagtaa tgcaattgtt ttaacgtagt atgcatgata
     7201 tcatcaggcc aaatttcatc gctggggatc atcaggtata catcgtgaat tttattggcg
     7261 agggtcacta attctctgtc gatatcattg ggatcgtaat gtggtttgaa atcatcaatg
     7321 ccttctattt ctcgcatcca aaaatgcatt ttaaatttcg cgagtggttc aaatgcgaac
     7381 tgaagaaata ggggagtgtc gatggcagaa tattggatac gacggtcttc atgattaagc
     7441 aattcatcag aagacttata tatcatttct atatgtttga taaacgatgc cttggtgcca
     7501 tcaggagcaa cgtaacggaa gttcaccagg ccttttattt catctgtaaa ataggcgata
     7561 ggtatcttat aatgattaca taatgttata gcctcatcaa tgctcatggc tgtttcacca
     7621 cgaatgcgtc ggtaggcact atcctggctg atattgagga tatcaatcaa ctggtcggcc
     7681 aaagaaatat gagccggcag cattcctttt aatcgtccga taaaatccag ttgataggga
     7741 ttttcctgtg gcagtgtgtc tttcatcgtc cgcaatttgc gaaaaatgca tgatatgtgc
     7801 aatgatggta cccatttttg aacaaaatgt aattgatgac ctgatgcatt ttgattaatg
     7861 aaaaaatggg aaagtttata aaatgtaagg gcgccacacc cctggggcgt agcgcctttt
     7921 cttacttgaa cgtctctgcg acaagacagc actaaaacgt gcacgtcttt cagattagtg
     7981 tactgctcaa atatttttat ttcaaaggtt gcaaggcttt tgcatagggt gtgtataact
     8041 ctgtttatcg gtggcagaag ggctatatat ctttaccatg caagattggt ggcgactaac
     8101 cgatacggaa ggttgcccta agagggaacc cggtgtgaat ccggggcagt ccccactgct
     8161 gtaattccgc agtctatgct ttcggaagcg caataccact gttcaattga ttgaatggga
     8221 aggttttccg aagggcggaa gagccagaag acctgccaat gcctgcacga tcatctcttt
     8281 tggggcaaaa gggatgtggc acagcgaaca tgctgtatta atagattgcc tctattattt
     8341 catttagaaa aacaacctat tatgaagaaa ttcatgtacc tgattctcgc ggtgatgatt
     8401 accacctccg cattttctca agcctacgtt tcgcgagtgc tcgtgttgaa cgaagggtat
     8461 ttcgattaca ttaccaatga catcattgtt ccggtgaccg ttggcgctta tgatccacaa
     8521 acagacactt atactaccat tcaaactatt gatgaagcca ggttcgcttc tgatattgct
     8581 attgatggcg attactatta tgtggctgct gatcattttt tgcagaaata tgatttgctt
     8641 accaacgccc tcatcagcag cattgatgtt gaaggcatac gtcaaatcgc aattacaaat
     8701 gatgagatca ttgttacgcg tggtgaatat ttggtaacgc tgccatcata tattcaggtg
     8761 tatcaaaaag aaacgatgac gcttgattat gaaattcctg cagatgtgat accgcacact
     8821 actgaaggtg ttatcgtaaa agatggtaaa gcttacatcg cagttaacaa cggtttcgtt
     8881 tttggtgggg aagtgggata tattgccgta ttagacctcg caaccgcata tgttgaaaat
     8941 atcattgacc tgggtgctga tggcatcaat ccggataatc tgatgatcga aggagatcaa
     9001 ttgtttacac tcaacaacaa agattttaca ggaagttctg tttcctcata caaaatcgga
     9061 acaggcgaac tggcaactac taaccttgca aatatttctt caggatgtgg cacatctgct
     9121 atatccggtg gaagcattta ttatcaggaa ttatttggca ctaccatcag taagttcgac
     9181 cctgcaacgg aaacgataac agatgaaaat gaatatggca tcagtttcta cggattgagc
     9241 ttcgacccgc tcaatggaca gatgtacact tccgaaacag actatttcag ctatggaaaa
     9301 gtatatgtat ataatactga tggtacattg tctacaagct tcgatgccgg cgtgtctccc
     9361 ggaaattttg catttgatgt caggatggaa tcaggtgtaa aagacctcaa atcagacttc
     9421 ctaaaaatgc agccaaatcc tgcaactgaa caaataagcc tgaaggcaga tttcaatatt
     9481 gaaacagtaa gtatcagcaa tattcaaggt gcctcttgtt taactttgga tggttctcat
     9541 gctgatttaa tgttgattga tatcactaaa ctgcctgcag gacaatatat cctttccgct
     9601 actggtgatt caagtactca gagcgccatg tttattaaac aataatgcat gataaacggg
     9661 taagactatt gatggtttta cccgtttgat ttttatgaga ctctcacttg tcgccatact
     9721 catttgtttg ctaactaccg gttgtcgacc aactgattca accccggagt tacctcatgg
     9781 gaaacgtgta gctgtgcaat ttgctcgcgg attcattatt gagcaaatag gtgatagtta
     9841 tttagtcact gtgcgcgatc ccttggatac aacgcacatc ctggcaacgt acaggttaaa
     9901 gcacgataac aaaaaaggta aagaccaaat caatattccc gtatcgcgac tggttagttt
     9961 ttctacaacg catatcgggt ttttagatgt acttggtgtt caaaatacgc ttgttggatt
    10021 tagtggtact acctatatct gcaatgactc ggtgagaaaa atggttgatg aaggaaaaat
    10081 agttgaagtt ggaaatgaag gtggaatcga ccatgaaaaa attgtgatgt tgcatcctga
    10141 tatcgtcatg acatacctga caggaaatgc ggcttacgat caagtggaca agctgtatac
    10201 attgggatta cagcctgtga taaataatga atttatggaa ttgtcgccat taggacaggc
    10261 ggaatggatc aaatttattg ctgtgtttta tgataggttg gatgaagcta acgcgatatt
    10321 cgacaaagta atgactgaat atattgctac ttcatcactg gttggtgaac gtggtgatag
    10381 accaactgtt tttaccggca tggcttataa aggtgaatgg acaattcctg gaggaaagag
    10441 ttttgcggca ggatatattc atgatgcagg agggaaatac ttatgggaat cggattcaaa
    10501 gaccggtaat tttccggtga gctttgagac catgttaagt atagcaggcg aagcagaaat
    10561 ttggttgcac cccggggcat ctgcgtcaag tgcagaaatt cttgccgccg atgaacgtta
    10621 tgcttctttt gatgctttaa aaaccggtca catcttcaac aacaatgcaa ggttatgcga
    10681 aggaggcggt aatgattatt gggaacgtgg aattgttttt ccaaatgaag tactcgccga
    10741 tcttgtagcc atatttcatc ctgaggtttt gaaagatcat acatttacct tttacacgca
    10801 tttaaggtga agcgaaatca gggcatatgg cctttgacga tattgttggt cttgacaata
    10861 ttattattct tcgctgatct cctggttggc tctactccga ttacgatatc tcagttgttt
    10921 gcaaatttgt taggcaaaca agataatgat gatatcaata ccattatctc cttattcaga
    10981 cttcctaaag catatacggc acttttagta ggtgctgcat tgcctgttgc aggattgttg
    11041 atgcagacat tcttcagaaa ccctgttgca ggtcccgata ttcttggtgt aacggcaggg
    11101 tcaagtctat ttgttgcaat agtaatgatg acaggtggtc tgttttttgg cattgatgcc
    11161 ttttatggaa tgactgttgt gctgtctgct attgcaggtg cagttgtgtt attattaatt
    11221 attcttttgg ttgctgcgcg attgagagac accatgacat tactgatatt cggaattatg
    11281 tttggaatgg ctatcagcgc aattgtcagc ataatacaat attacagcga acgggatgcc
    11341 ttgcgcctgt ttatgctttg gacattcgga agcctgggcg gtgttacaca tgcgcaatta
    11401 attataatgg tgccatt
//
LOCUS       DAOQHY010000107        11217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2880_length_11217_cov_5.211611, whole genome shotgun sequence.
ACCESSION   DAOQHY010000107 DAOQHY010000000
VERSION     DAOQHY010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11217
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2880_length_11217_cov_5.211611"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="PLL28_09590"
     CDS             <1..1093
                     /locus_tag="PLL28_09590"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNF69616.1"
                     /translation="TLLYIGVEKGQSAKVDQVIAIVGKEGDDVQALLASAGTVAKAEE
                     KAPSTETKQAEATIEVAATTQDQSASNTEDGRIKASPLAKKIAKEKGLDLHAISGSGP
                     EGRIIKKDVEQANPIAVEQTESAKPQINFAPVAGVESYEEVPVSQMRKVIAQRLSQSK
                     FTSPHFYLSISVDMDKAMAARTSMNAVSPVKISFNDLVVKASAMALRRHPQVNASWLG
                     DKIRFNHHIHIGVAVAINDGLVVPVLRFADQKSLAQLSAETGDLAERAKSRKLQPQDW
                     EGNTFTISNLGMMGIEEFTAIINPPDACILAVGGINQVPVVKNGQVVPGNVMKLTMSC
                     DHRVVDGAVGAQFLKTLKEYLEDPVRMLI"
     gene            1131..1493
                     /locus_tag="PLL28_09595"
     CDS             1131..1493
                     /locus_tag="PLL28_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNF69617.1"
                     /translation="MHKRTLVLGASENPERYANKAVRRLNAAGHDVVAFGKTGDHIGD
                     TKIEREWLPWEDIDTVTLYLNPVHQEAYYDKIIALAPKRIIFNPGTENDAFEALAHKH
                     GIETLEACTLVLLSIGEY"
     gene            1526..2200
                     /locus_tag="PLL28_09600"
     CDS             1526..2200
                     /locus_tag="PLL28_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNF69618.1"
                     /translation="MKTPLSHTEENYLKAIFKLSELNSSNVSTNAIAEKLSTTAASVT
                     DMLKKLASKKLLHYEKYYGVTLTEKGKKNAKDLIRKHRLWETFLVDKLRFSWDEVDEL
                     AEQLEHIRSEILTDRIDQFLGYPKFDPHGDPIPDKHGNIAYHEDVTLESMKKGESGII
                     VGVIDHSTEFLRYLDKVDLVIHTRIHISEDEEYDQSKTILVNHKHETVISQKVAANLL
                     VRKTGN"
     gene            complement(2203..2634)
                     /locus_tag="PLL28_09605"
     CDS             complement(2203..2634)
                     /locus_tag="PLL28_09605"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNF69619.1"
                     /translation="MENQTPLDQVDLSVEQNYELASQGKRFLNFIIDYIIILGIVVAV
                     ILIIGGFSSESGFYLVFYPTFWLYYVVMEGSTGRTIGKFLTGTVVVRNIDLQAPSISQ
                     CMGRSISRFVPFEQFSFLGSQTGWHDNWTNTMVVNVKSLVR"
     gene            2777..4663
                     /locus_tag="PLL28_09610"
     CDS             2777..4663
                     /locus_tag="PLL28_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HNF69620.1"
                     /translation="MNLGKNHSATGKSLEDVHGTVDTTARGTGWRRFLAFIGPAYLVS
                     VGYMDPGNWATDLAAGSGFGYTLIWVLLMSNLMAILLQSHSARLGIVRGQDLAQASRT
                     SYPPIINIPLYIFAEIAIAACDLAEVLGLAIGLNLLFGMPLLWGVSIAVLDCILLLLI
                     LNFGIRKMEAFIISMVFIMGASFFVEVFMAKPDVGGIVNGFAPSIPSETALYIAIGII
                     GATVMPHNLYLHSALVQTRKFENTNEGKKRAIKFNLIDSTLALNLAFFVNASILILGA
                     AVFFSNGLTEVADIQDAHKLLEPLLGSSLAPILFAVALIASGQSSTITGTLAGQIIME
                     GYLNLRITPWLRRLITRLLAVVPAFLVIWLKGEEETGELLILSQVILSMQLGFAIIPL
                     IHFVSDKKKMGSFAIGTITRIASWVTAIIIVSLNIKLVYATISDLLDKPNNWWVYAFV
                     IPVALYGIVLLLYIAIKPFVSSKTYEPKLINTIEPNMEFAIKGAFKKIAVTLDFSSQD
                     TPTIAQAIAAGGKEAEYILIHIVESAGAITFGKDTGDMESRKDFQQLKAYVDELMRLG
                     YKAKAELGYGRPTRAIPEIVKQLEADMLVMGAHGHRGLKDLILGETINTVRHRISIPL
                     LAVK"
     gene            4720..5115
                     /locus_tag="PLL28_09615"
     CDS             4720..5115
                     /locus_tag="PLL28_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69621.1"
                     /translation="MINPFQIGDKKVFSIVVTHHDVAAFESGIVHEVYSTFCLARDAE
                     WSTRLFVLDMKESDEEGIGTFIEIYHRSPAAVESQVDFTATLLEVNGHEVICSFTATC
                     GERIIAEGRTGQKILKKEKVNRLMASIAK"
     gene            5117..5575
                     /gene="smpB"
                     /locus_tag="PLL28_09620"
     CDS             5117..5575
                     /gene="smpB"
                     /locus_tag="PLL28_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNF69622.1"
                     /translation="MKPAEINIRNKRATFEFEIIDRYVAGLMLRGSEIKSVREGKVSM
                     NDAYCQFRDGELWVKNLNISEYKEATVWQHEPLRMRKLLLTKKELSKLLAKVKERGFT
                     IIPLRLFVSDRGFAKIEIALARGKKVHDKRASIKEKDEKRDLARTMRKYQ"
     gene            complement(5634..6449)
                     /locus_tag="PLL28_09625"
     CDS             complement(5634..6449)
                     /locus_tag="PLL28_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69623.1"
                     /translation="MKKMMIVAIATVMSTGLFAQNMPKAGYNGFGFTITGLSNVGFGN
                     YGNTILGNAVISDPAGVVAAGYDTPLNALIPQQAFMYKRYYDNGMASRLQLGINSMSS
                     KTSVSDSTGVPDEYAVTDESLKGFTIGLGLGMEKHMSTEAAKVDPYVGADLLFGFLTG
                     FNYEGTTDVTGDGYSSNSSLTVSYPGGMGLALNAICGFNYFFSDNISIGAEAGLGFGM
                     VKTGGEWTSDFSYTSNVGGTSTTTTSADRGEYSNSMSGVQVNSRGAVNLIVYW"
     gene            complement(6701..7357)
                     /locus_tag="PLL28_09630"
     CDS             complement(6701..7357)
                     /locus_tag="PLL28_09630"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNF69624.1"
                     /translation="MVPGLDTYKHKGMRQGLVDLLRDKGISDERVLAAINAVPRHTFM
                     DPAFLGHAYEDKAFQIGEGQTISQPYTVAYMSEWLEVEPGMRILEVGTGSGYQACVLA
                     EMGGNVYSIERFKKLSDTARKQLDILGYQGVKTFFGDGYEGLPELAPFDRIIITAAAK
                     EIPQALLKQLRPGGIMVFPLGNDDDQLMIKLTKTGEYDYRKEVGDNFRFVPMLPGKVI
                     "
     gene            7541..8395
                     /locus_tag="PLL28_09635"
     CDS             7541..8395
                     /locus_tag="PLL28_09635"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF69625.1"
                     /translation="MKKLLLPVMLLVTTVAGAQATLGLNAQKTYDITPADFGLVFDTI
                     RITTDDGASLFGWHLYPGEKNDKSSKKSVIISHDGNGNMANALEQAGKFLGLGFHVFM
                     YDYRGFGKSSDFTVSPKFYIYQQFATDLNATIEYVKKYHALLSIDLYGFGIGGGLSLG
                     VGANNLKVHRIIADNPYTTFETVQTRYEQKTGTRIMMPIGYNVTLLEPKMALEDKGDH
                     LRGILLIIGEQGSIMTQADMMELQAMKKKVTSIYTVKGVDNDKNFSSNRDEYFKQIKA
                     FYDKFPDS"
     gene            8501..9901
                     /locus_tag="PLL28_09640"
     CDS             8501..9901
                     /locus_tag="PLL28_09640"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69626.1"
                     /translation="MIRIKFGTDGWRAIIAQEFTTDNVARVSKATADWIKQSSSTPSV
                     VIGHDCRFGGELFTDVAARVFCEEGVKVFVAKDFVSTPMVSFGAHALNAVAGVVITAS
                     HNPPSYNGFKLKSGYGGPTIPEEIAKVENIIPDHHPIPQTSLAEYEAAGKLQWVDLEE
                     MYYQHVVKSFDLDTINNSGIRLGYDAMYGAGQRVALRIFKNIVPLHCDDNPGFHGQAP
                     EPIHRNLIALSELVKNDPTIKCGLANDGDADRIGMYDGDGNFVDSHHIILLLIHYLHK
                     YKGYTGKVVIAFSVSEKVKRLCEHYGLDYEVTKIGFKYICGKMVIEDVLLGGEESGGI
                     AVKGHVPERDGIWCGLLLMEFMAKTGKNLTELIKEVYDITGSFCFDRNDLHLTDALKN
                     SIIENCKTGKYTAFGKYTVDRLEDVDGFKFHLDANSWVMIRPSGTEPLLRVYAESPTR
                     AETDSILSTVKSILLG"
     gene            10241..10639
                     /locus_tag="PLL28_09645"
     CDS             10241..10639
                     /locus_tag="PLL28_09645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNF69627.1"
                     /translation="MQENEISEIVIKEACYVHSAFGPGLLESVYEIALFTRLSQRGLK
                     VRKQVPIPVLLDGVTLEVGFRADLIVEEKLILEIKSVEALHPVVYMQLKTYMRFTGIH
                     VGLVLNFYVNSMKDGIKRYVYQYNGPTLSK"
     gene            10684..>11217
                     /locus_tag="PLL28_09650"
     CDS             10684..>11217
                     /locus_tag="PLL28_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69628.1"
                     /translation="MLHWPMIKPMPNDMIVEIEISSNLVKKNRPHHQPNPLHLESMIA
                     PPPQTPQTPRFISPPLQTPQTPCFISPPLQTPQTPRFISPPLQTPQTPRFISPPPQTP
                     RFIQHCNLTKLIITFLGFFLLTPIFSQSKLTNFVTPADTFNAKRLGLILGSETTLYTS
                     ATIALYQYWYKDYPLNHF"
BASE COUNT         3341 a         2340 c         2359 g         3177 t
ORIGIN      
        1 aacactgttg tatatcggtg tcgaaaaggg tcaaagcgca aaagtggatc aggtgatcgc
       61 catcgttggt aaggaaggtg atgatgttca ggctttgctt gcttctgcag gaacagtagc
      121 caaggcggaa gaaaaggcgc cttcaacaga aactaaacag gctgaagcaa caatcgaagt
      181 tgcagccaca acacaagatc aatctgcttc gaatacagaa gacggaagaa ttaaagcttc
      241 gccactggca aagaaaatag ccaaagaaaa aggactcgac ctgcatgcaa tctccggttc
      301 aggtccggaa ggccgtatca ttaaaaaaga cgtcgagcag gcaaatccga ttgctgttga
      361 acaaacagaa tctgcaaaac ctcaaattaa tttcgctccg gtagccggtg tcgaaagtta
      421 tgaagaagtg cccgtgtcgc aaatgcgaaa agttattgca caacgtcttt ctcaaagcaa
      481 attcacttca ccacatttct acctgtcgat cagtgtagat atggataagg caatggcagc
      541 acgcaccagc atgaatgctg taagccctgt taaaatatca ttcaacgact tggtagtgaa
      601 agcatcagca atggcgttgc gacgtcaccc tcaagtaaat gctagctggc tgggcgataa
      661 aattagattc aatcatcata tccatatcgg tgttgctgtc gccattaatg atggcctggt
      721 agtgccggtg cttcgttttg ccgaccagaa atcactggct caattatcag cagaaaccgg
      781 cgatcttgca gaacgcgcaa aaagcagaaa attgcagcca caggattggg aaggaaatac
      841 atttaccatt tctaaccttg gaatgatggg aatagaagaa tttactgcca tcattaatcc
      901 tcctgatgct tgtattttgg cggttggcgg tatcaatcag gttcctgttg ttaagaacgg
      961 acaagtagtt cccggaaatg taatgaaact gactatgagc tgcgatcacc gagtggttga
     1021 tggggcagtt ggtgctcagt tcctcaaaac cttgaaggag tatctggaag atccggtgcg
     1081 tatgctcatc tgaggctgaa ctatttcgtc accgattaca ttttattatt atgcataaac
     1141 gaacgcttgt tcttggggct agtgaaaatc ctgagcgcta tgctaataaa gctgtgcgga
     1201 gactaaatgc cgccggtcat gatgtagtgg ccttcgggaa aaccggcgat catatcggcg
     1261 atacaaaaat cgaaagagaa tggttgccgt gggaagacat tgataccgta acattatatc
     1321 ttaaccctgt gcatcaggaa gcctactatg ataaaataat tgccctggct cccaaacgta
     1381 tcattttcaa ccccggaacg gaaaacgatg ctttcgaggc cttagcgcat aaacatggca
     1441 ttgaaactct tgaagcctgt accttagttt tgctgtcgat aggagaatat tgaaattgtt
     1501 cactaacggc attcatgcag ctgatttgaa aacacccctt tctcataccg aagaaaatta
     1561 cctcaaggca atatttaaat tgtcggagct gaatagcagc aatgtgagta ctaacgccat
     1621 tgccgaaaaa ttaagcacta ctgccgcatc ggtaaccgac atgctgaaaa aactggcttc
     1681 aaaaaaatta ttgcattacg agaaatatta tggggttacg cttacggaaa aaggaaaaaa
     1741 gaacgcaaaa gacctgattc gcaaacacag gctttgggaa actttccttg tagataaatt
     1801 gcgctttagt tgggatgaag tagatgaact tgcagaacaa ttggaacata ttcgctccga
     1861 aattttaacc gatcgtattg accagtttct cggatatccg aaattcgatc cgcatgggga
     1921 tcccattccc gataaacatg gcaatatcgc ctaccacgaa gatgttacac tcgagtcaat
     1981 gaagaagggt gagtccggta tcattgttgg tgttattgat cacagcacag aatttctacg
     2041 ctacctcgat aaagtagacc ttgtgattca tacacgtatt catatctccg aagacgaaga
     2101 atatgaccaa agcaaaacca ttctcgtgaa tcataaacac gaaacggtca tctctcagaa
     2161 agttgcggca aacctgcttg ttagaaagac aggtaattaa tcttatctca ccaatgattt
     2221 gacattaact accatcgtat tggtccagtt atcatgccat ccggtttgac ttcccaaaaa
     2281 agaaaattgt tcaaacggta caaagcgtga aatacttctt cccatacatt gactgatgct
     2341 cggggcctga agatcaatat ttctgacaac cactgtgcct gtcaaaaatt tgccgatagt
     2401 cctgccggta gatccttcca ttaccacata gtaaagccag aatgtaggat agaaaacgag
     2461 gtaaaatcct gattcacttg aaaatcctcc aataattaga ataacagcaa ctactattcc
     2521 taaaatgatg atgtaatcaa tgatgaaatt gagaaatcgc ttgccttgtg atgcgagttc
     2581 gtaattctgc tctaccgaca gatctacctg atcaaggggt gtttggtttt ccatgagtgt
     2641 attttcaata aaaatacaat tttcttccaa ctgccaaaat ctaaacggaa caaaaaagca
     2701 tattttgggg ttttaaaata aattttagac taatctaaaa aatattttat ctttgcctta
     2761 aaatcaggca tttgaaatga atttagggaa aaaccatagc gccacaggta aatcgttgga
     2821 agatgtgcac ggtacggtag ataccactgc ccgtggcaca ggctggaggc gctttctggc
     2881 gtttattggt cctgcgtacc tggtaagcgt tggctacatg gatccgggca attgggctac
     2941 cgaccttgct gccgggagtg gctttggcta taccttgatt tgggtcctcc tgatgagcaa
     3001 tcttatggcc atccttttgc agagtcacag cgcgcgactc ggaattgttc gcggacagga
     3061 ccttgctcag gccagcagaa ctagttatcc gcctatcatc aatattccac tgtacatttt
     3121 tgctgaaata gccattgcag cctgtgacct tgctgaggtg cttggactgg ctatcgggct
     3181 aaatctctta tttggaatgc ctcttttatg gggggtgtct attgctgttt tagattgtat
     3241 actgctgtta cttatcctca atttcggcat ccgcaaaatg gaagctttca tcatatcgat
     3301 ggtgttcatt atgggagctt cgttttttgt ggaagtgttc atggcaaagc ctgatgttgg
     3361 tggaatagta aatggatttg cgccatcaat accttctgaa actgctttat atatcgccat
     3421 cggtatcata ggtgctaccg taatgcctca taatttgtat ttacatagtg ccctggtgca
     3481 aacgcggaaa tttgaaaata caaacgaagg gaaaaaacgt gcaataaaat ttaatcttat
     3541 agatagtacg ttagcattga atcttgcttt ttttgttaat gcttcaatat tgattttagg
     3601 tgccgcagta tttttctcta acggactaac agaggttgca gatattcagg atgcgcataa
     3661 attgttagag ccattattgg gatcatcgct tgcacctatt ttgtttgccg ttgcattgat
     3721 cgcctccgga caaagttcta ccattaccgg tactctggca ggacaaatta ttatggaagg
     3781 atatttaaat ctgcgcatta ccccttggtt gcgaagattg ataactaggc ttctggctgt
     3841 agtgccggca tttcttgtga tttggctgaa gggtgaagaa gaaaccggtg aactgctcat
     3901 cctttcacag gttattttga gtatgcaact tggtttcgcc attataccgc ttattcattt
     3961 tgtaagcgat aaaaagaaaa tgggctcttt cgctatcggc acaatcacgc gaattgcttc
     4021 ttgggtaact gcaataatta ttgtgagttt gaatattaaa ctcgtgtatg ctaccattag
     4081 cgatctgctg gataaaccga ataattggtg ggtatatgcc tttgtgattc cggtggcttt
     4141 atatggcatt gttttgttat tgtatattgc aataaaacct ttcgtcagtt ctaaaacgta
     4201 tgaacctaaa ctgatcaata ccatagagcc aaatatggaa ttcgccatta aaggtgcttt
     4261 caaaaaaatt gcagtgaccc tcgatttttc atctcaggat acacctacta ttgctcaagc
     4321 aattgcagca ggtggaaagg aagcggaata cattcttatt catattgttg aaagcgccgg
     4381 tgccatcaca tttggtaagg ataccggtga tatggaatcc agaaaagatt tccaacaact
     4441 aaaggcctat gtcgatgagt tgatgcgatt aggatataaa gccaaagctg aattgggtta
     4501 cggtcgccca acacgcgcaa ttcccgaaat tgtgaaacag ctggaggccg acatgctcgt
     4561 aatgggtgct cacggtcaca gaggattaaa agatttgatc cttggtgaaa cgatcaatac
     4621 cgtgaggcat cgtatttcaa tcccattgtt agccgtaaaa taataagctt caaccggtta
     4681 aatatcgcgc agtaacaaag gcttgtgtat ctttaccgcg tgattaaccc atttcaaatt
     4741 ggtgataaaa aggttttttc aattgttgta acacatcatg atgtggccgc atttgaaagt
     4801 ggtattgtac acgaagtgta tagtaccttt tgccttgccc gcgatgctga atggagtaca
     4861 agattgtttg tgcttgacat gaaagaaagc gatgaagagg gtataggtac cttcatcgaa
     4921 atctatcatc gctctcccgc agcggtggaa tcgcaagttg attttacggc taccttgctc
     4981 gaggttaatg gtcatgaagt tatttgctca tttactgcta cctgcggtga acgcattatt
     5041 gctgaaggca gaacgggaca aaagatttta aagaaagaaa aagtaaacag gctgatggca
     5101 tccatcgcca aatgagatga aacccgcaga aataaatatc agaaataaac gcgccacctt
     5161 cgaatttgag atcatcgaca ggtatgttgc cggattaatg ttgcgtggct cagaaattaa
     5221 aagtgttcgc gaaggaaaag taagcatgaa tgatgcctat tgccaatttc gcgatggtga
     5281 attgtgggtg aagaatttaa atatctccga atacaaagaa gcaacggttt ggcagcatga
     5341 acctcttcgc atgcggaaat tattgctcac taaaaaagaa ttatcaaaac ttctggcaaa
     5401 agtaaaggaa cgtggattta ccataattcc attgagatta tttgtgagcg acagaggatt
     5461 cgcaaaaatt gaaatcgcct tggcccgcgg aaaaaaagta catgataaac gcgcatctat
     5521 caaagaaaaa gatgaaaaac gagaccttgc cagaaccatg cgcaagtatc aataaataat
     5581 aaaataaaaa atcccggcat tgaccgggat tttttattca taagagaaat gaattaccaa
     5641 taaacgatta agttcacagc gccgcgactg ttcacctgta cgcctgacat tgaattgctg
     5701 tattcgccac gatcggcaga tgtagtagta gttgacgtgc caccgacatt actggtatag
     5761 ctgaaatcgc ttgtccactc accaccggtt ttcaccatac caaatccaag acctgcttca
     5821 gcaccgatag aaatattatc gctgaagaaa taattgaatc cgcagattgc atttaatgca
     5881 agtcccatac cgccgggata tgacactgtc aggctgctgt tagaactata accatcaccg
     5941 gttacgtcgg tagtaccttc ataattaaat ccggttaaga atccgaaaag taaatcggct
     6001 ccaacatatg ggtcaacttt ggcagcttca gtgctcatat gtttctccat accaagacca
     6061 aggcctattg tgaaaccttt taatgactca tcggtaactg catattcatc aggaacacct
     6121 gtagaatcac ttactgaagt ttttgaactc atgctattga tacctaattg caagcgactg
     6181 gccattccgt tatcataata acgcttgtac ataaatgcct gttgcgggat caatgcattc
     6241 agtggagtat catatcctgc tgcaacaacg cctgcagggt cactgattac ggcgttacct
     6301 aaaatggtat tgccatagtt accaaaacct acgtttgata agccggtaat ggtaaatccg
     6361 aaaccattgt agccggcttt aggcatgttc tgagcgaaca agcccgtgct cattactgtg
     6421 gcaatagcca cgatcatcat ctttttcata ctgtttgttg ttttgtttac ttggttatat
     6481 aaatgggtta aattgaatgc aaatcattgc cacattatcc atgacgataa tgcaactccg
     6541 gtattgtgct aatccgttcg ttaaaaattt gtgtgtgtaa aaaatcacta ttggcgtgta
     6601 acaacagcga tttaaaaact atgatgccgg ctgtgacatc atttacttat tacaagatga
     6661 ctcatcgcgg ctgaacgatg cacgacacct tggtaaatat ttaaattact ttgcccggaa
     6721 gcatgggtac aaatctaaag ttatcaccca cttccttccg ataatcatat tcacctgttt
     6781 ttgtcagttt tatcattaat tggtcatcgt cattgccaag tgggaacacc atgatgcctc
     6841 ccggacgcag ctgttttaac aatgcctggg gtatctcttt tgccgccgct gtgatgatga
     6901 ttctgtcgaa tggcgcgagt tccggcagtc cttcataccc atcgccaaag aaggttttaa
     6961 caccctggta acccaatata tcgagctgtt tacgagccgt atctgataac tttttaaacc
     7021 gttcaataga gtatacattt ccccccattt ctgccaaaac acaagcctga tacccactac
     7081 cggtccctac ttccagaatg cgcattccgg gttctacctc caaccattca ctcatgtaag
     7141 ctaccgtata cggctggctg atggtttgtc cctccccaat ctgaaaggct ttgtcctcat
     7201 aagcatgccc taaaaacgcc ggatccataa atgtgtgtcg cggaacagca ttgatagcag
     7261 ccaaaacccg ctcatcactg attcccttat ctcgcaaaag atctaccaat ccctgccgca
     7321 tgcctttatg cttgtatgtg tctaatcccg gtaccatggc gctgcaaagt aaacaactgt
     7381 tcggtggctt actttccact aatcactact aattatccac atttcgctgc gcggttcaga
     7441 aaatgcaagt agggagaatt atttttaagg cacgaaattt gacaatcctt ggattgcaca
     7501 tttcattttt aaacaaaaag caaatcgcac tatgcaattg atgaaaaaac tccttttacc
     7561 ggtaatgctc ttggttacca cagttgccgg cgcacaagcc acccttggcc taaacgctca
     7621 aaaaacctac gatatcactc cggcagattt cggactggtt ttcgatacca ttcgcatcac
     7681 tactgatgat ggcgcaagct tattcggatg gcatttatat ccgggtgaga aaaatgataa
     7741 atcgtctaaa aagtcggtta ttatcagcca cgatggaaat ggaaatatgg ctaacgccct
     7801 cgaacaagca ggtaaattcc ttggtctcgg attccatgtg ttcatgtatg actatcgcgg
     7861 attcggaaaa agcagcgatt ttaccgtgag cccgaaattt tacatctatc agcaatttgc
     7921 tacagacctt aatgcaacca ttgaatacgt gaaaaaatat cacgcccttt taagcatcga
     7981 tttatatggt ttcggcatcg gcggtggatt gtctttgggt gttggtgcaa acaacctcaa
     8041 agtacatcgc atcattgcag ataaccccta tacaactttc gaaacagtgc aaacacgcta
     8101 cgagcaaaaa accggtacac gcattatgat gcctatcgga tacaatgtaa ccttgctcga
     8161 acctaaaatg gcactcgaag ataaaggcga tcaccttcgt ggaatcctcc ttatcattgg
     8221 cgaacaagga agtatcatga ctcaagccga tatgatggaa ttacaagcta tgaagaaaaa
     8281 agttacatcc atctataccg tgaaaggcgt agataatgac aagaacttca gcagcaaccg
     8341 cgacgaatac tttaaacaaa tcaaagcatt ctacgacaaa ttccctgact cataatgagt
     8401 tattaaaata ttgtaaagcc accgatagtt actgtcggtg gcttttactt ttacgccctt
     8461 tgttatctat ctttggtctg aaataaacat acagcacatc atgatccgca tcaaatttgg
     8521 aaccgacggt tggagagcta tcattgcaca agaatttact acagataatg ttgcccgtgt
     8581 aagcaaagcc actgccgact ggattaagca atcttcttca actccatcgg tggtaatagg
     8641 ccatgattgt cgctttggag gtgaattatt cactgatgtt gcagctcgcg ttttttgcga
     8701 agaaggtgtg aaagtatttg ttgctaaaga ttttgtttct acaccaatgg ttagctttgg
     8761 cgctcatgcc ttaaatgctg ttgccggtgt ggtgatcact gctagtcata accctccatc
     8821 ctacaatgga tttaaattga aaagcggcta cggcggacca acaattcctg aggaaattgc
     8881 aaaagttgaa aatattattc ccgaccacca tcctattccg caaacatctc ttgcagaata
     8941 tgaggccgca ggaaaattgc agtgggttga tctggaggaa atgtattatc agcatgttgt
     9001 aaaaagcttt gatttagata ccatcaataa ttcaggtatt cgccttggtt atgatgccat
     9061 gtacggagca ggacaacgtg ttgcgcttcg catatttaaa aatattgtcc ctctgcattg
     9121 cgatgacaat cccggatttc atggtcaggc acccgaacct attcatcgta acctcatcgc
     9181 attaagcgag ttggttaaaa atgatcctac cattaaatgc ggactcgcca atgatggtga
     9241 tgccgataga attggcatgt acgatggaga tggaaatttt gttgattcac atcatatcat
     9301 cttattgctc attcattacc tgcataaata caaaggatat accggtaaag tggttatcgc
     9361 attcagcgtt agcgaaaaag taaaacgttt gtgtgaacac tatggactgg attacgaagt
     9421 aaccaagatc ggatttaaat atatctgtgg taaaatggtt attgaagatg tattactcgg
     9481 cggtgaagaa tccggcggta tcgcagtgaa agggcatgtg ccggaacgtg atggaatctg
     9541 gtgtggactg ttgttgatgg aattcatggc gaaaacagga aaaaatctta cggagctcat
     9601 taaagaagtg tatgatatta caggttcttt ctgtttcgat cgcaatgatt tgcacctcac
     9661 ggatgcgttg aaaaatagca tcattgaaaa ttgcaaaacc ggcaaataca cagcgttcgg
     9721 aaaatataca gttgatcgcc tcgaagatgt tgatggattt aaattccacc tcgatgcgaa
     9781 tagttgggta atgattcgcc catcaggaac agaacctctg ctccgcgtct acgccgaatc
     9841 cccaacccgc gctgaaaccg acagtatcct ttcaacagta aagtcaattt tattgggttg
     9901 atgtattaaa gctttaaaag gcaattaccg taatggtagt tttagttgcg gaatttacag
     9961 agttgttgta aatcgcggag ttagcggagt ttgcagagtt gttatcaatt gcggagttag
    10021 cggagtttgc ggagttgttg taaatcgcta agttgacgga gttggcagag ttgttgtcaa
    10081 tcgctgggtt agcggagttt aacttgtttc tggagttggt agcgttagca tcgttttccc
    10141 tgtaagggaa gctagcacaa aatttaatct ttattcgagt taatcgctta ctatcgggta
    10201 aataaaattt caatatgcat ttttgcttta aataagtgag atgcaggaaa atgaaatttc
    10261 ggaaatagtt atcaaggaag cttgttatgt gcatagtgca ttcgggccgg gtttattgga
    10321 gtcggtgtat gaaattgctt tatttactcg tttatcgcaa cgcggattga aggtgcgaaa
    10381 acaagttccg attcctgttt tgctcgatgg cgtaaccctt gaggttggat ttagggctga
    10441 ccttatagtt gaagaaaagt taattttaga aattaaatct gttgaagctt tacatcctgt
    10501 cgtttacatg cagttgaaaa catatatgcg atttaccggc atacacgtag gtttggtatt
    10561 gaatttttat gtgaactcca tgaaagacgg tatcaaacgc tacgtatatc aatacaacgg
    10621 cccaacactt tctaagtaag ttgattgctt gaataactat caggaattgt aaatttgctt
    10681 caaatgcttc actggccaat gataaagccc atgccgaatg atatgatagt tgagattgag
    10741 ataagcagca atctagtcaa aaaaaatagg cctcatcatc aaccaaaccc gttgcatctt
    10801 gaatctatga tagctccacc accgcaaact ccgcaaactc cgcgcttcat ctccccacct
    10861 ctgcaaactc cgcaaactcc gtgcttcatc tccccacctc tgcaaactcc gcaaactccg
    10921 cgctttatct ccccacctct gcaaactccg caaactccgc gctttatctc cccacctccg
    10981 caaactccac gctttatcca acattgtaat ttgacaaaat taataatcac ctttttgggt
    11041 ttttttttat taactccaat tttttcccaa tccaaactca ccaattttgt cacccctgct
    11101 gatacattta atgcaaagcg tttaggattg atattgggat ctgaaacaac gctttataca
    11161 agtgctacaa tagcacttta tcaatactgg tataaagatt acccgcttaa tcatttt
//
LOCUS       DAOQHY010000108        11177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2893_length_11177_cov_4.983726, whole genome shotgun sequence.
ACCESSION   DAOQHY010000108 DAOQHY010000000
VERSION     DAOQHY010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11177
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2893_length_11177_cov_4.983726"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="PLL28_09655"
     CDS             <1..644
                     /locus_tag="PLL28_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HNF69629.1"
                     /translation="SYAHVPWKSKGQRRYTDEDVPVAEEKLQMYQTGSAYLHQLGFSH
                     IGMDHFALPTDALMLAYQVGKLHRNFMGYTTTNHKLVIGLGASAISDTWDAFAQNEKG
                     VEAYQDAIASGRLPIINGHNLNETDQLIRKHILDLMCRDTTTLVEEQLPAGTFDAIME
                     RLSGFVEDGLISIEGGKISILPSGRLFIRNICSAIDLHLHSGSRQGNTFSKAI"
     gene            701..1915
                     /locus_tag="PLL28_09660"
     CDS             701..1915
                     /locus_tag="PLL28_09660"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HNF69630.1"
                     /translation="MRPTLSHFRSILYLFTASMFFASCAVDPPMVNDDSFRGEIISST
                     LLHDWSITQTDSALSDYDPSLLTYPNDYHIKIYRVVYRTIDAAGNETQASGAVVIPYG
                     VANGAAQCLYMHGTVVERFEVPSYESDELVLGMLYGANGFVTSLPDYLGMGDSPGRHP
                     YCHSATEASAGIDLLRATKTLCANEGVSLNNQLFIFGYSQGGHAAMAATKAIQEDYAD
                     EFTITASAPMSGPYDLSGAQTDYVLQDAPYGAPFYLPYLMFGYNDIYKMYDNYADYLA
                     SPYDTLLPSLFDGTHSGWEIDAVMPVIPKNIIKPEVLEDFINNPNNPFRVALRENDLT
                     DWTPQIPMIMYYCGGDELVNYQNSIIAQDAFVANGSTTTSLYQPSATASHTDCAEPCF
                     IFANIWFSSLRQ"
     gene            complement(2022..4139)
                     /locus_tag="PLL28_09665"
     CDS             complement(2022..4139)
                     /locus_tag="PLL28_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HNF69631.1"
                     /translation="MKSILTLLCGVLLSTSLPAVTVTSPDRTLLLNVDLDKSGALHYS
                     LSRNGAVVIAPSLMGFSLKDLGELTADFEAVDTVYNSIDTYWEPVWGEVAKIHDNHNE
                     MTFTVRHKKTGIVMEVIFRMFNDGMGFRYQFPVQSYMKYFTVLNEKTQFCLNGDHQAW
                     WIPGDYDTNEYFYTESKLSDVHAITASTRNREIATRSPIGDNYVQSPLMMKSSDGLYI
                     NIFEAALVNYPAMHLEVDREKHILTSRLATDAVGNCAYLSTPCHTPWRTVIVSDKATD
                     ILGSHIILNLNEPCAIANTDWIKPMKFVGIWWEMHVGTASWNYSDVNNINLETTDWTK
                     LPPNGHHGATTERTKYYIDFAAANGFDGVLVEGWNVGWEDWFGNWKENVFDFVTPYPD
                     YNVQELSAYAKSKGVSLIMHHETSASVTNYERRLQQAMQFMVSNGYPAVKTGYVGRII
                     PRGEYHDGQWMVNHYVYVAQEMAKHHLMVDAHEPVRPTGLQRTYPNWLACEAARGNEF
                     NAWSQGNPPEHETILPFTRLMGGPMDYTPGIFEVQMSTYGQQTGNQVHTTLAKQLALY
                     VTMYSPLQMAADIPENYLKYADAFQFIKDVAVDWQDTKYLEAEPGDYLTVARKEKGGR
                     NWFVGAITDDKARRTSIKLDFLDDKQVYIATIYGDAEGADWKTNPKAYMITQQKVTNM
                     STIKLELAPGGGAAVSIMPIQEN"
     gene            complement(4196..4930)
                     /locus_tag="PLL28_09670"
     CDS             complement(4196..4930)
                     /locus_tag="PLL28_09670"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HNF69632.1"
                     /translation="MIRNTTNTSPGQEPLFLSKWTLDNLHGSTISIESQPYLLFTASE
                     TFRVSGFAGCNLITGGFTLSGVDSIDFGEIASTLMACPQYATEQDFLTALQEANRWMI
                     SGGQLFLLQGSDVVASFTGSTIDVAEEAGNMAVNGTWELSYISGPRIAFDALYPQQKP
                     TLVISMPNTKATGNSGCNSFSVDVKIKDKTILFGPIAATKMYCEGSGESTYFDNLGKV
                     DTWQMDDNNNLALMIGDVVMMRFIRK"
     gene            complement(5032..5709)
                     /locus_tag="PLL28_09675"
     CDS             complement(5032..5709)
                     /locus_tag="PLL28_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69633.1"
                     /translation="MRRIKLLLIAFVSIISLRAQPELEFCRVAPIVYFDSVDNKPMLN
                     LPVGGKWIDSLEFSLNGNYKNSFVSVDIGVQGSGAEILRESDSLWRISLFAYFDFSSD
                     SDTAQEIEVARLILTKNEAGYICKTDLSFRPDPVELKGEQLHNLFSSCANNNLYKLSA
                     FDIGESEWDTLSLLINDLFVLSLHGDALALKEFNDMLILFPAVKMYELHHYYMSARAV
                     LCLYGMK"
     gene            complement(5696..6265)
                     /locus_tag="PLL28_09680"
     CDS             complement(5696..6265)
                     /locus_tag="PLL28_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69634.1"
                     /translation="MKTFCTLICFLLFVLPAEAQQDSVFLKYPVDIDSLGKAIYSVDT
                     ILFRAYHDGDILIGTTVIPGTNNSMNLMNHGYRLHNIRLMPCDGGDVFEVVSRVNAVR
                     ETDSTLEVDVTITDNCCFNFLCDARIDEINVMHLFYTGYGMNCSCFCCFGLTYVFERW
                     NFTNDPLIDAVMIADDKKSLYNISRDAKD"
     assembly_gap    6474..6573
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6731..7426)
                     /locus_tag="PLL28_09685"
     CDS             complement(6731..7426)
                     /locus_tag="PLL28_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HNF69635.1"
                     /translation="MQTFKSDLSDKEFPIKEMVYATAVRPTIMTLIKADHPAFDEGKG
                     LLSLSELSFYREKYIADYLKSEIADLSDLEHTVVKSLQDNSLLSDRADLEGDTNITTG
                     QRLADNVARFGGSWRFIILFGLFLAIWIMINALLLSNKGFDPYPFILLNLMLSCLAAI
                     QAPLIMMSQNRQEEKDRQRAKKDYMINLKSEMEIRLLHEKLDHLLMHQQQKLLEIQQF
                     QIEMMNDIYNRLK"
     gene            7552..8688
                     /locus_tag="PLL28_09690"
     CDS             7552..8688
                     /locus_tag="PLL28_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNF69636.1"
                     /translation="MIIGILGEGQSDQRVAIAAEHIPSLKALKAEKILIESNAGLAAG
                     SPDADYVAKGAEIASRAEVIAKSDMLIKIHPVGSDELAKMRGKVLIGGFNPLVNTSFV
                     SACLENGVSAFSLDVIPRTTRAQAMDILSSMATVAGYKAVLTAASHLPKFFPMFMTAA
                     GTITPAKVVILGAGVAGLQAISTAKRLGAVVEASDVRTAAREEVLSLGAKFIEVEGAT
                     EDKSAGGYAVEQSEDFKKRQQAKVQEHASKADVIICTAQIPGRKAPILLQKETVAQMR
                     PGTVIVDLAASTGGNCELTENGKTININGVTIIGDSNLASTMPQDASKMFGKNILNFL
                     KIMIDKDGNTALNFDDDLITGTCLAHEGNIRSERVKAQMSTVTA"
     gene            8709..9026
                     /locus_tag="PLL28_09695"
     CDS             8709..9026
                     /locus_tag="PLL28_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNF69637.1"
                     /translation="MILLDIFQNFLASLDTWDIIYIVLLAAFLGVEVISHVPAILHTP
                     LMSGANAIHGVVIIGSIIVMGHAESTLALVLGFLAVILGTLNVVGGFVVTNRMLQMFK
                     KKK"
     gene            9150..10541
                     /locus_tag="PLL28_09700"
     CDS             9150..10541
                     /locus_tag="PLL28_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNF69638.1"
                     /translation="MTTLDILKLCYLLGSVSFIVGLKMLAKPDSAKRGNMLAAIGMGI
                     AIIGTILFHRNEEDESIGNLAWIFGGIAIGTVVGYLAAMKVKMTQMPQMVSLFNGMGG
                     ACAALIGIVEMDTHNPEVFGERFTIFLGLMIGTVSFAGSIIAYGKLEGKVKDFGSKFQ
                     QWFNNTFIFAILALLVYASLTPDFMPSSVIWIIFGASMLYGILFVLPIGGADMPVVIS
                     LLNSFTGVAAAFGGFLYHNQVMLTGGILVGSAGTILTVLMCNAMNRSLWNVIIGAFSG
                     SSGGGKSTEGLSIREINVTDTAILCAYAQKVTIIPGYGLAVAQAQHACHEFEKVLEDK
                     GVEVNFAIHPVAGRMPGHMNVLLAEADVPYDKLKEMEDINPHLPNTDVVIVIGANDVV
                     NPAAETDPSSPIYGMPIIKANTAKNIIVMKRSMAKGYAGIENELFYDNKTRMLFGDAK
                     ATLEKLVAEVKNV"
     gene            complement(10552..10788)
                     /locus_tag="PLL28_09705"
     CDS             complement(10552..10788)
                     /locus_tag="PLL28_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69639.1"
                     /translation="MKSTGTCPKCNSTKLYTNAGLAKRGDRSMIALSSWKGLYVDVYV
                     CSQCGFIEEYASTRELENPTTLEKLKTNWKKVNE"
     gene            10902..>11177
                     /locus_tag="PLL28_09710"
     CDS             10902..>11177
                     /locus_tag="PLL28_09710"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNF69640.1"
                     /translation="MAQPTKKMVVIIGGGAAGFFCAINLARLRPDLEVHILEKSQQVL
                     SKVKVSGGGRCNVTHACFNPEELIENYPRGGKELLGPFHHFQPADTIQ"
BASE COUNT         3203 a         2364 c         2259 g         3251 t
ORIGIN      
        1 attcatatgc acatgtgcca tggaaaagca aaggtcaacg cagatataca gatgaagatg
       61 ttcctgttgc tgaagaaaaa ttacaaatgt atcaaacagg gagtgcctat ttacaccaat
      121 taggattttc gcatataggt atggatcatt ttgctttacc tactgatgct ttaatgctcg
      181 cctaccaagt aggtaaatta catcgcaatt tcatgggtta tacaactact aatcacaaat
      241 tggtaattgg tttaggtgca tctgccatca gtgatacctg ggatgcattt gcacagaatg
      301 aaaaaggtgt tgaagcatat caggatgcta ttgcttcagg gcgactgcca ataataaacg
      361 ggcataatct taatgaaaca gatcaactta tccgcaaaca tattctggat ttaatgtgtc
      421 gcgacaccac cacacttgtg gaagaacaat tacctgccgg cacatttgac gctatcatgg
      481 aaagactgtc gggatttgta gaagatggat taatcagtat agaaggcggc aaaatttcga
      541 tattaccctc aggccgctta tttatcagga atatctgttc tgctattgat ttgcatcttc
      601 atagcggaag caggcagggc aatactttca gcaaagcgat ataaggtaat ctcaatttca
      661 ataccttagc ttatctttgg taagataagt tatataatac atgagaccta ccctttcaca
      721 cttcaggagc atcctttatc tgtttactgc ctcaatgttt tttgcatcat gcgccgttga
      781 tccgccaatg gtgaatgacg atagcttcag aggtgagata atttccagca cattgttaca
      841 tgactggtca attacacaga cagattctgc tttatcggat tatgatcctt cattactgac
      901 gtatccaaat gattatcata tcaaaattta tcgtgtggta tatcgcacaa ttgatgcagc
      961 aggaaatgag actcaggcat ccggtgctgt agtaatccct tacggtgttg caaacggtgc
     1021 agctcagtgt ttatatatgc acggaacggt agttgaacga tttgaagtgc cgtcttatga
     1081 aagtgatgaa ttagtgttag gtatgttgta tggtgcaaac ggatttgtta ccagtttacc
     1141 ggattatctc ggcatgggcg attcaccggg gcgacatcca tattgtcatt cagcaacaga
     1201 agcatctgca ggaattgatc ttcttcgtgc aactaaaaca ctttgtgcaa atgaaggtgt
     1261 ttccttaaat aatcaattat ttattttcgg ttacagtcag ggtgggcatg cggcaatggc
     1321 agcaaccaaa gctatccaag aggattatgc agatgaattt acgataacgg caagtgcgcc
     1381 catgagcggc ccttatgatt tgagtggcgc acaaactgac tatgtgcttc aggacgctcc
     1441 gtacggtgct ccgttttatt taccgtattt aatgttcggg tacaatgaca tatataagat
     1501 gtatgataat tatgccgatt atctcgcatc accttacgac accctattac cttcactttt
     1561 tgatggcaca catagtggtt gggaaataga tgctgtaatg cccgttattc ccaaaaatat
     1621 cataaaacct gaagtgctgg aggatttcat taacaatccg aataacccgt tccgcgtagc
     1681 attgcgtgaa aacgacctca ccgactggac gccacagatt ccgatgatca tgtattattg
     1741 cggtggcgac gaactggtta attatcagaa tagtattatt gcacaggatg catttgtcgc
     1801 aaatggttca acaaccactt cactttatca accttctgca acagcatcgc acacagattg
     1861 tgctgaaccg tgttttattt ttgctaatat atggtttagc tcccttagac aatagtgaaa
     1921 taatatgaca attgttatct aacaggctta tataagatgt tgcgcaggtg caaacaaata
     1981 gcaaaacgta tattgcagaa attgtcaaat attaaacgcc atcaattctc ctgaatcggc
     2041 ataatactca ctgctgcacc tccacccgga gccagttcta attttatggt cgacatattt
     2101 gtcacttttt gttgtgtgat catatatgcc tttggattcg ttttccagtc agctccttcc
     2161 gcatctccgt aaattgttgc gatgtatact tgcttgtcgt caaggaaatc gagtttgatg
     2221 gatgttcttc gtgccttatc atctgtaatg gcaccaacaa accagtttct gccacctttt
     2281 tcctttcttg caacagtgag ataatcgccg ggttctgctt ccaggtattt cgtatcctgc
     2341 caatctacag ctacatcttt aataaattga aatgcatcgg catactttaa ataattttca
     2401 ggtatatctg cagccatttg caatggtgaa tacattgtaa catataatgc taattgcttg
     2461 gcaagtgtgg tatgcacctg attacctgtt tgctgtccat aggttgacat ctgcacttcg
     2521 aaaatgccgg gagtataatc cattggtccg cccattaatc ttgtaaatgg caatattgtt
     2581 tcatgttcag gcgggttacc ctggctccat gcattaaatt catttccgcg cgctgcttcg
     2641 catgccaacc agttcggata agtgcgttgt aaaccggttg gacgaacagg ttcatgcgca
     2701 tcaaccatca gatgatgttt tgccatttcc tgtgccacat acacataatg atttaccatc
     2761 cattgtccgt cgtgatattc gcctcttgga attatacgac caacataacc ggtttttacg
     2821 gcaggatatc cattgctcac cataaattgc attgcttgct gcaatctgcg ttcataatta
     2881 gttaccgatg cagaggtttc atgatgcatg atgagactaa cgccttttga tttagcataa
     2941 gctgataatt cctgcacatt ataatcggga tatggcgtca caaaatcgaa tacattttct
     3001 ttccagttgc caaaccaatc ttcccaacct acattccagc cttcaactaa cacaccgtca
     3061 aatccatttg ctgccgcgaa gtcgatatag tatttagtgc gctctgttgt tgcgccatgg
     3121 tgaccattag gtggcaattt ggtccagtcg gtggtttcca gattaatatt atttacatca
     3181 ctgtaattcc atgatgctgt gccaacatgc atttcccacc aaatgccaac aaatttcatt
     3241 ggtttaatcc aatcggtatt ggcaatggca caaggttcat ttaaattaag aataatatga
     3301 gatcctaaaa tatctgtcgc tttatcactc acaataactg tgcgccatgg cgtatgacaa
     3361 ggagtagata aatacgcgca atttccaaca gcatctgtag caaggcggga agttaaaata
     3421 tgtttctcac gatctacttc aagatgcatg gcaggataat taacaagtgc tgcttcaaaa
     3481 atattgatat acaagccatc agatgatttc atcataaggg gcgactgcac ataattatcg
     3541 cctattggag aacgcgttgc aatttctctg tttctggtgg atgcggtaat tgcatgcaca
     3601 tcgctgagtt tgctttcggt ataaaaatat tcgttggtat cataatcgcc cggtatccac
     3661 catgcctgat gatcgccatt taagcaaaat tgtgttttct cattcaacac cgtaaaatac
     3721 ttcatataag attgcaccgg aaattggtat ctgaaaccca taccgtcgtt aaacatcctg
     3781 aaaatcacct ccattacgat acctgttttt ttgtgtcgaa ccgtaaaggt catttcattg
     3841 tgattgtcat gaattttcgc cacttcaccc caaaccggtt cccagtatgt gtcgatggaa
     3901 ttgtatacgg tatctacggc ttcaaaatcg gcggtaagtt caccaagatc tttcaaagag
     3961 aatcccatga gcgatggcgc tatcaccact gctccattgc gagagaggga ataatgcagt
     4021 gcgccggatt tatccaaatc aacattcaac aataatgttc gatcaggtga tgttactgtt
     4081 actgcaggta aggaagtaga taacaataca ccacatagca gagtcaggat ggatttcatc
     4141 cttcaaaatt atcatgtaat gatcagaaaa ggtagtactt ccgaagccgg aaaccttatt
     4201 tgcgaataaa tcgcatcatc acaacatcgc caatcatcaa agcaaggttg ttattatcat
     4261 ccatttgcca ggtatccacc tttccgaggt tatcgaaata ggttgattca cccgaacctt
     4321 cacaatacat ttttgtagcg gcaatcggtc cgaataagat ggttttatcc ttgattttca
     4381 catccacaga aaacgaattg catccggagt taccggtagc cttcgtattt ggcatggata
     4441 ttactaaagt tggtttttgc tggggatata aggcatcaaa tgcgatgcgt ggaccactga
     4501 tataagacaa ttcccatgtg ccgtttacag ccatattccc ggcctcttct gccacatcaa
     4561 ttgttgatcc ggtaaaagaa gccacaacat cagatccttg taacagaaat agctgacctc
     4621 ctgagatcat ccatctgttt gcctcttgca gtgctgtcaa aaaatcttgc tcggtagcat
     4681 actgcggaca agccatcaag gtagaggcaa tttcaccgaa atcgatgcta tccactccac
     4741 tcaaagtaaa tccgccggtg atcaggttac agcctgcaaa cccgctcacc ctgaatgttt
     4801 cgctggccgt aaacaacaaa tatggctgcg actcaatgct tatagtggat ccatgcaaat
     4861 tatccaaagt ccattttgat aaaaacaaag gttcctggcc cggggaagta ttggtagtgt
     4921 ttctaatcat ggtgcaggat gaaataaaca gcacgattat aaagagtggt atcgatttgc
     4981 gaaacatagt ggtaattgaa atgcaaagtt acgatataaa cgttgaatta atcacttcat
     5041 gccatataaa cagagcactg cacgggcgct catatagtaa tgatgcaatt catacatctt
     5101 aacggcagga aataatatta gcatgtcatt gaattctttc aatgccaatg catctccatg
     5161 caaactcaaa acaaacaaat cgttgatgag taaactaagg gtatcccatt cgctttcacc
     5221 aatatcgaat gctgataatt tatataggtt gttgttggca catgaggaaa acaagttatg
     5281 caattgttca cctttcaact ctaccgggtc cgggcgaaaa gaaaggtcag ttttgcaaat
     5341 gtatccggct tcgttttttg tcaaaatcaa tcgcgccact tcaatttctt gcgctgtatc
     5401 cgaatctgat gaaaaatcaa agtatgcaaa caaagaaatc cgccacaagg aatccgattc
     5461 gcgcaaaatt tcagctccgg agccttgcac gccaatatct acagagacaa atgaattttt
     5521 ataattacca tttaaagaaa actctagtga atcaatccac tttccgccta caggtaaatt
     5581 cagcattggt ttattatcga cggaatcgaa ataaacaata ggagcaaccc tgcaaaattc
     5641 caattcgggt tgcgcacgaa gtgaaataat agatacgaaa gcaatcaaca acagtttaat
     5701 ccttcgcatc acgggatata ttatataaac tctttttatc atcagcaatc atgacggcat
     5761 caatcaaagg atcattcgta aaattccaac gttcgaaaac ataagtcaac ccaaagcaac
     5821 agaaacaaga gcaattcatg ccataacctg tgtaaaacag atgcatgaca ttaatttcat
     5881 caattcttgc atcacataaa aaattaaaac aacaattatc tgtgatggtg acatccactt
     5941 ccagtgtaga atctgtttcc cttactgcat taacccgcga aaccacttca aacacatcgc
     6001 caccatcgca gggcataagg cgaatattgt gaagcctata cccatgattc attaagttca
     6061 tggaattatt cgtacccgga ataacagtgg ttccgataag gatatcacca tcatgatatg
     6121 ctcgaaataa tatggtatcc accgaataaa ttgctttgcc caggctatct atatctacag
     6181 ggtatttcag aaatacggaa tcctgttgtg cctctgccgg taggacaaat aacaaaaagc
     6241 atatcaatgt acaaaaggtc ttcatgtgca gtttaattac taaaacgata aacccgggaa
     6301 acaagtatct gatcgaagtt acatgatgtt ttgtacttgc tcattcaaac accgaagcac
     6361 ctgccagaat cctggcaaac atattccagg agggcttttc cggatcaaaa tgctccttaa
     6421 agaaatagta tctgcctcta agcccatgct ctgtatagtc ggcaatagct tctnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaaataat ggcaaagtat tgttctcgaa
     6601 atcttccgga tttgtcttca aatcttttct caattcatgc acatatcgga taaaatcctc
     6661 cattgtttgc acgctatctt cgtaaaattc gtcttcaggc atattgttgt tttgatggat
     6721 ggaaattagt ttatttcaac ctattataaa tatcattcat catctcaatc tgaaattgtt
     6781 gaatctctaa cagcttctgt tgctgatgca tgagcaggtg atcgagtttt tcatgtagga
     6841 ggcgtatctc catttctgac ttgagattga tcatataatc tttctttgcc cgctgcctgt
     6901 ccttttcctc ctgacggttt tgactcatca taatcagtgg cgcctgaatc gcagccaaac
     6961 acgagagcat caggttgagt aaaataaacg gatatggatc aaatccttta ttcgataaca
     7021 acaatgcgtt gatcatgatc catattgcca gaaacagccc aaacagaatg ataaatcgcc
     7081 aactacctcc aaatcgtgca acattatctg caagacgctg acctgttgtg atatttgtat
     7141 ctccttccaa atctgcacgg tcgctgagca gtgaattgtc ctgcaagctt ttcactaccg
     7201 tatgctccag gtcactcaag tcagctattt cacttttgag gtaatccgca atgtatttct
     7261 ctctgtaaaa actcaactcg ctcaatgaaa gcaagccctt accttcatca aaagccgggt
     7321 gatcggcctt gataagcgtc atgatggttg ggcgcacagc agttgcataa accatttcct
     7381 taatgggaaa ctccttgtct gaaaggtcgc ttttgaatgt ttgcatcgca tttcaggact
     7441 tgttgatatc ctgttataca aagctacacg atacctgatt ttgaaggtca aaacggcagg
     7501 cttatgccca tttttaacta tctttgcggt cctaatcatc caaatcttca catgatcatc
     7561 ggaattctgg gagagggtca gtcggatcag cgagtggcaa ttgcagctga acacattccc
     7621 tctctaaaag ccttaaaagc agagaaaata cttatcgaat ccaatgccgg attggcagca
     7681 gggtcaccgg acgctgacta tgtcgcgaaa ggtgccgaaa tcgcttccag agcggaggtg
     7741 attgccaaaa gcgatatgct gatcaagatt catcctgtcg gaagtgatga actcgccaaa
     7801 atgcgcggaa aagtgctcat cggaggcttc aacccgttgg taaacacctc gtttgtgagc
     7861 gcttgtcttg aaaacggcgt ttcagctttc agcttggatg taatccctcg aactacccgt
     7921 gctcaggcaa tggatattct cagctcaatg gctaccgtag ccggttataa ggctgtgttg
     7981 actgccgcaa gccatttgcc aaaattcttc ccgatgttca tgaccgctgc cggtactatt
     8041 acgcctgcta aagtggtgat attaggtgcc ggtgttgccg gattgcaggc tatttctact
     8101 gcaaagcgtc tcggcgccgt tgtagaagcc agcgatgtgc gcacagcagc ccgcgaggaa
     8161 gtgctttcgt tgggagccaa attcattgaa gtggaaggtg ctaccgaaga taaaagtgca
     8221 ggcggatatg cggttgaaca gagtgaagac tttaaaaaac gtcaacaggc taaagtgcaa
     8281 gaacatgctt ctaaagccga tgtgatcatc tgcaccgcgc aaattcctgg tcgtaaggca
     8341 ccaattcttc ttcaaaaaga aacggttgct caaatgcgtc cgggaactgt aattgttgac
     8401 ctggcagcaa gcaccggtgg caattgtgaa cttacagaga atggtaaaac catcaatatc
     8461 aatggcgtga caattattgg cgatagcaat ctcgcatcaa ccatgccgca ggatgcaagt
     8521 aaaatgtttg gtaaaaacat tctcaacttc ctcaaaatca tgatcgacaa agacggtaat
     8581 actgctttga attttgacga cgatctaatt accggcactt gcttagcaca tgaaggcaat
     8641 attcgcagcg aacgtgtgaa agcacaaatg tcaaccgtaa ctgcctaacc aaaaaagaat
     8701 tatcacttat gatactgctc gatatttttc agaatttcct cgccagtctc gacacctggg
     8761 atatcattta cattgtgttg cttgcagcat ttcttggtgt tgaagtgatc agtcacgtac
     8821 cggcaatcct ccacacacca ctgatgtcgg gcgctaacgc cattcacggt gtagtaatca
     8881 tcggatccat tatcgtgatg ggtcatgccg aaagtactct ggcactggtg ctcggattcc
     8941 tcgccgttat cctcggaacc ctcaacgtag ttggcggctt cgttgttaca aatagaatgt
     9001 tgcaaatgtt taaaaagaaa aagtaaatgt ggttgtgtgt tcatgtgtcc atgtgttcat
     9061 gaaaatgcat tatcctgatt acaactcacc aatcaccatt caccacccgc ctatcggcga
     9121 ggcaagctca ccattcacca ctcaccaata tgacaactct agatatactt aaattatgtt
     9181 acctcctcgg cagtgtttct ttcattgtcg gattgaagat gttggcaaaa cctgattctg
     9241 ctaaacgcgg aaatatgttg gctgccattg gtatggggat cgccattatc ggaacgattt
     9301 tatttcacag aaatgaggaa gatgaaagca taggtaacct cgcatggata ttcggtggta
     9361 tcgccattgg tactgttgtt ggttacctgg cagccatgaa agtgaagatg acacagatgc
     9421 cgcagatggt atcgctattc aatggaatgg gtggagcttg tgcagcctta attggcattg
     9481 ttgaaatgga tacgcataat ccggaagtat tcggtgagcg ctttactatt ttcctgggat
     9541 tgatgattgg aacagtttct ttcgcaggtt ctatcattgc ttatggaaag ctcgaaggaa
     9601 aagtaaaaga tttcggttct aaatttcaac agtggtttaa taatactttc attttcgcca
     9661 ttcttgcttt attggtttat gcatcattaa cacctgattt tatgccatca agtgttatat
     9721 ggatcatttt tggtgcttcc atgttgtatg gtattttgtt tgtattgcct atcggtggtg
     9781 cagatatgcc ggtggtgata tcattactca actcatttac aggtgttgcc gccgctttcg
     9841 gtggtttctt atatcataac caagtgatgt taaccggtgg tatccttgta ggttctgccg
     9901 gtaccattct taccgtattg atgtgtaatg ccatgaaccg tagtttgtgg aatgtgatca
     9961 tcggtgcatt tagtggaagt tccggtggtg gtaaatcaac tgaaggtctg agcatccggg
    10021 aaataaatgt taccgatacc gctattcttt gtgcttatgc gcagaaggta accatcattc
    10081 cgggttatgg tcttgccgtt gctcaagcac aacatgcgtg tcacgaattt gaaaaggtgt
    10141 tggaagataa aggtgttgaa gtgaattttg ctattcaccc tgttgccggt cgtatgccag
    10201 ggcatatgaa cgtgttgctt gcagaagcag atgtgcctta tgataaattg aaagagatgg
    10261 aggatatcaa tcctcacctg ccaaatacgg atgttgtaat tgtgatcggt gccaatgacg
    10321 tggtaaaccc tgctgcagaa acagatccgt catcacctat ttatggcatg ccgatcatca
    10381 aagcaaatac cgcgaaaaat attatcgtaa tgaaacgttc catggctaaa ggttatgccg
    10441 gcatagaaaa cgaattattc tatgataata aaacgcgtat gctgtttggt gatgcaaaag
    10501 caacactcga gaaactggtt gctgaagtaa agaacgtctg atcaatattg atcattcgtt
    10561 tacttttttc cagttggttt tcagcttttc taatgtagtg ggattttcca attcgcgtgt
    10621 ggaagcatat tcttctataa acccgcattg ggaacaaaca tacacatcca catacaaccc
    10681 tttccagcta cttaatgcta tcatgctgcg atcgccacgc ttggctaacc ctgcattggt
    10741 gtacaactta gtgctattac attttgggca tgtacctgtt gatttcatga ttgcaatttg
    10801 attacgaata taagccaatt ttctgcctca ttatccaact aaccaacacg ggattcgtta
    10861 ttataagaag cattcaataa taccatatac ctttgtcgta catggcacaa cccactaaaa
    10921 aaatggtagt gataataggt ggaggcgcag caggattctt ttgtgcaata aatcttgctc
    10981 gcttgcgtcc cgaccttgaa gtgcatattc ttgaaaaatc gcagcaggtg ttgagcaaag
    11041 taaaagtttc gggcggtggg cgatgtaatg ttacgcacgc ctgttttaat cccgaagaac
    11101 ttattgaaaa ttatcctcgt ggagggaagg aactgcttgg tccttttcat cattttcaac
    11161 ctgcagatac gattcaa
//
LOCUS       DAOQHY010000109        11162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2901_length_11162_cov_5.264248, whole genome shotgun sequence.
ACCESSION   DAOQHY010000109 DAOQHY010000000
VERSION     DAOQHY010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11162
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2901_length_11162_cov_5.264248"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            266..1708
                     /locus_tag="PLL28_09715"
     CDS             266..1708
                     /locus_tag="PLL28_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69641.1"
                     /translation="MANNNNLILKSKFWTQYKTLSDDEAKDFADFVSAGIFARTPKAG
                     RLAKVLRVLRQEPLTVAEVYSRTFEKSAFNEQVLRNYLSELNDALHEFLAMVAMHQRP
                     VLQNWMYLESVRVAAYQPELEKQLEQDRYQPELKTTDDMFAYFRQLQIVDEYAATQAH
                     KKRYDLLVPAMHMLTDHYITAMLRMYCEAVNRKALRSQNYDEEALQFFLTQVAYIEQS
                     RPLTMMAQLFKSVLNIIMFPAEENYFEILKNLLQKHIIDLDKSMAREICLYAQNYCIR
                     HINLNHSTYMQELLSLYDFMILHNVISEGPFMTQYTFKNYITLSLRLHQFDSALHFIK
                     QQQHRLHESVREQAARYNLAAVYFEQGEHGAAMEELFYIQMQDPIYYLDSRAILLKIY
                     FAQQDVEAVGSLYHAVKTYLLRNKQLPRKQAELYRNLFMLSNKLMMLSQNWKYLSPED
                     AMSRKLKLTSKIELSPVANKPWLMTQLNAL"
     gene            complement(1849..2334)
                     /locus_tag="PLL28_09720"
     CDS             complement(1849..2334)
                     /locus_tag="PLL28_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69642.1"
                     /translation="MKRSLYIVMFLITGILFAGCPYSAEIALDERPNIPVDKAMLGKW
                     ELRSSSDYTYVITAKNDFVYSIMKKSTSDGSETVYEGFLSDINGKKFLNIYETGSTTP
                     TYYFYLFTQTGNDRFKLDPVTDNIDETFTNSRDMRNYFAKNMDVSFFFDKDADEYLRS
                     N"
     gene            2402..3430
                     /locus_tag="PLL28_09725"
     CDS             2402..3430
                     /locus_tag="PLL28_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNF69643.1"
                     /translation="MIWTAMGATPSGESLRRIASMPNYTKGQFQNIEHTPVMSEDYHM
                     GKVMMDMMKRNSARTPATPLPNVKTDLLHLENNALVWFGHSAYLLKINGKHILVDPVF
                     SGHASPVPFMIKAFKGSNSYGVADMPEIDMLVITHDHYDHLDYETVVALKSKVKEVVC
                     SLGVAAHLIKWGYQESHIHELYWHESKTVFENFTFTAEPARHFSGRGITRNKSLWCAF
                     ALQTPELTVFIGGDSGYGKHFKEIGTRYNGFDIALLECGQYNPAWKYIHMMPEETVLA
                     AQDIQAKIFVPVHWGKFALSLHTWQEPADRVVAAAQRAEMPYATPKIGEAYVIGAGGQ
                     THHWWQMR"
     gene            complement(3651..4568)
                     /locus_tag="PLL28_09730"
     CDS             complement(3651..4568)
                     /locus_tag="PLL28_09730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69644.1"
                     /translation="MITLTVIQPVITACSEDFNKMHKHQSGAFCDSCNKVVHDLSALN
                     TEQLITFIQQHPGACGRVQSNAMRMSVTVKDDIKPSKSFSMRFAFALLVVFGPLLFSC
                     TEEQHHQIKEIAQQEFFENEAINSMDTATEEIAIQNPEYTAVKQTILDEQQTFISEIT
                     DTTEIELETVNVSVPSNSKTQCFFAGGLSYTKIVNVISIETDTTDLQPIKESESLLTN
                     RTLSLGVYPNPTVSQAEVKYSLKQSGPLLVTIFNLAGQEMLPLVNDQNVDAGQYTHNI
                     DVASWPSGVYLVVLVSGTEQKNFKFTVAH"
     gene            complement(4687..5265)
                     /locus_tag="PLL28_09735"
     CDS             complement(4687..5265)
                     /locus_tag="PLL28_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69645.1"
                     /translation="MKKLLLALAMTLSAFTYAQSDDTEHHPFRIELEPSGFIYRGYGV
                     QALYDLNYIGTMIGGLYFSSLDVPDWTHKGMFDEVSDTADVRLGFEAALVYRYRFLIG
                     NGASAPYVGAIAGYEYFDITQAPYPKTVRLSTFVVTPYIGYEVYVWNQRLYVNPQVRS
                     VFYINQQSSDNDRPETLSNLFILPTISLGYTL"
     gene            complement(5329..6303)
                     /locus_tag="PLL28_09740"
     CDS             complement(5329..6303)
                     /locus_tag="PLL28_09740"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HNF69646.1"
                     /translation="MKGGCGIMGIKRVYLLMICLLPYYIFCQATEEQSKLTGTDIDLV
                     GELIVFDGQLGSLSVHRHDNYIYRDIYFDTPDFDLYNNGYSLRIRSRYFNADSIVYAI
                     QLKSEMTNISAVRMEVEEADLDIYTISNGETRLPLTTLLNRLFQSVDTSTNFIKTNLN
                     KQDVALLHQWMIFKSESTITPFQTLHAIFPDDKNLIDRLQPVTFGSEYRSRCHLYISI
                     DSLNEKDLIVQPLSQEEVPIFFNEHPEYIWVAEGSYDEAQFISLLTPNDRAISIVEFE
                     IENKLKPETTGTALIEACKSDLIKDHRLSIEYRSKYRQAVEGLGLAGN"
     gene            complement(6272..7915)
                     /locus_tag="PLL28_09745"
     CDS             complement(6272..7915)
                     /locus_tag="PLL28_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69647.1"
                     /translation="MKAFLLCVVFSSLLINSSKPSIPRGSLILNDHPKEVLRFIDDGY
                     LDLREIRNTCNFLTDPNITIYKGDSIPRRKNAFVRHIKQLNNTLETAWETQTPVNDSL
                     LEILYEIALFESSLKEKILASAHPNIGFDDQLSMWLNTQRSKSVFSVAIDNQINKTPT
                     AKTSPFWHPADSAGALNKRFDQLAKLKKIKAKKKMVVVFDDFSYDGSAPKINTLDLDM
                     DNGWVMKWGDEIHTDVAGSRIFAALGYDVDHPYYYGRDAAMLVFPKWQAVQTSEDLIN
                     MIKQIYHVDLSPFISTIGVIDDAMINDDNNLLPYANCTYMTFIECALEARPDRVKRIG
                     SFIPNNLHNDQRRALRGALLAHAWIGNWDTRESNTLLTQVHMGNYQYRISAVFSDLGT
                     SMGVDLHVVPPDFKTGAVNNFGWEVLRTYGNRIYFNEPMNALLSPYKDAQYADLLWMA
                     NLIAQIDGVALRKMVDDAHWPEPLATLYYYKLASRRENILNTFHIKDPHPIQYDIYYS
                     VKENGEYLIENGLLLKEIQPADHPEHFLKHEGRMRNYGN"
     gene            complement(7917..8411)
                     /locus_tag="PLL28_09750"
     CDS             complement(7917..8411)
                     /locus_tag="PLL28_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69648.1"
                     /translation="MSAISEISPLAFALIILISGTSFTCFWWLDSITHGKLAHSDITP
                     QELTTHRIILATSLFMMTSLFLMYWFEPLILLPFFIAGFITRTAHETMDEIHFHINRC
                     TPYESLLHLGMWLSILTNTTALFMWGFFTQYAGIESLSPILAFWALLIGISIIYISKQ
                     EWSR"
     gene            complement(8395..9297)
                     /locus_tag="PLL28_09755"
     CDS             complement(8395..9297)
                     /locus_tag="PLL28_09755"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNF69649.1"
                     /translation="MFIQPDISPSLPHNTSVLGPKVLELNHVSYRKLHYFHAAALSLF
                     FCGVIISPEQSIIDLSISTWFVLCGVFMIYRFNDIIDHAHGFRFNSKKITGSPIHLLF
                     IVQLICVATPLAWIYLSPFRLTLLSIIGVIGILYSVPLHFRGRVLRIKHTFLLKNISI
                     GVLWGLLPSLGADHITTPLILGLTWFAIMQVTMGSVLRDIADRVHDKLFEVQTLPVRW
                     GVSKTLFYAQIWNILSLTVFICICPEIYIAIAFLTPVIWRAWNIYKIGKSPDAARWTH
                     TINLLTCAVIFIMFIVSQLYVGNL"
     gene            9845..10957
                     /locus_tag="PLL28_09760"
     CDS             9845..10957
                     /locus_tag="PLL28_09760"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNF69650.1"
                     /translation="MNKKGSVKFVNPDQHLFYQTLKENVNRYFKENGISTHANAQMVT
                     KTIVLFIMYLAPYVLLMTTPMSYGLALGLWSLMGVAFAGIGMSVMHDANHGAYSDSPG
                     VNKMMGYSLNMVGSMVFNWKLQHNILHHTYTNITGLDEDIQAKAGMRFTPNVPLNKPH
                     RWQFIYVFGLYGIMTLYWIIFKDFFQLVRYRKQGVNAASSREYRMQFIKLLAIKAIYF
                     FAIIAAPIIWAGFSFWQVALGFVVMHVVGSFILSLVFQLAHTVEGTDHPTPDKDGIVH
                     NIWAVHQLQTTMNFARKNKVLSWYVGGLNFQVEHHLFPKICHVHYPKIAPIVKQTAEQ
                     FNIPYLENETFWIALRSHLRLLYDLGRIPKLDDIMD"
BASE COUNT         3342 a         2173 c         2351 g         3296 t
ORIGIN      
        1 atgagatcgc aaacactatc attcttcaca tcgcctccgg caaccaccag agggagtgat
       61 tgtgccttca tgttgcatgg aagcaccgct gtcaatgaca ttattgcggt aaataatatg
      121 gtgcgaatcc ttggcatggc gcgtttctgg tgacaaaatt gtcttctaaa taccctgaaa
      181 acaagaatta tattttataa ttttaaccac taaattaata tcaatatttc ataaagtatt
      241 gaataccaac aaaatatagt aaataatggc taataataat aaccttattt tgaaatcgaa
      301 attctggact caatacaaaa ctttatcaga tgatgaagct aaggattttg ccgatttcgt
      361 ttctgcagga atttttgcca gaacgcctaa ggccggacgc ctagcgaagg tgctgcgcgt
      421 cctcaggcaa gaaccactga cggttgctga agtatacagc cgcacatttg aaaaaagtgc
      481 tttcaacgag caggttttaa gaaattacct atcggagttg aatgatgccc ttcacgaatt
      541 tttggctatg gttgccatgc atcaaaggcc tgttctccaa aattggatgt acctggaatc
      601 agtgagagtt gctgcctatc agccggaact cgagaagcaa ctcgaacaag atcgttatca
      661 gcccgaatta aaaaccactg acgatatgtt tgcctatttc cggcaattac aaattgttga
      721 tgaatatgca gcaacacaag cacataaaaa gcgttacgat ttgcttgtgc ctgctatgca
      781 catgttaact gatcattata taacagcgat gttacgcatg tactgtgaag cagtgaatag
      841 aaaagcattg agatcgcaga attatgacga ggaagctttg caattctttc tcacgcaggt
      901 tgcctacatc gaacaatcga gaccactgac catgatggct cagttgttta aatccgtgct
      961 gaatattatt atgtttcctg ccgaagaaaa ctattttgaa attttgaaaa atttgctgca
     1021 gaaacatatc attgatttgg ataagagtat ggcaagagag atttgtctgt atgcacaaaa
     1081 ttattgtatc aggcatatta atctcaatca cagcacttat atgcaggagt tgctatcgct
     1141 atacgatttt atgatactcc acaacgtgat cagtgaaggt ccatttatga cgcaatatac
     1201 ctttaagaat tatataacac tgtcactgcg cttgcatcaa tttgactctg ctttgcattt
     1261 tattaagcag caacaacatc gtttgcacga gtctgtgcga gaacaagcag cgcgatataa
     1321 tcttgcagca gtatattttg aacagggtga gcatggcgct gctatggagg agttatttta
     1381 tattcaaatg caagatccca tttattattt ggactctcgt gccattttat tgaaaattta
     1441 cttcgcacaa caagatgtcg aggcagtagg atccttatat cacgcagtaa aaacatacct
     1501 gcttaggaat aaacagcttc cacgtaaaca agctgaattg tatcgcaacc tattcatgtt
     1561 gtcaaacaag ctgatgatgc tttcacaaaa ttggaagtat ctatctccgg aggatgctat
     1621 gtcgcgtaaa ttaaaactta cttcaaaaat tgaattaagt cctgttgcca ataaaccctg
     1681 gcttatgaca cagcttaatg cactttgatt tgcctgacgt ccacaaactt atatatggct
     1741 ctgaagggaa gtggctttgt tttgcctttg aacaccaata aatgccttca gatatgcgta
     1801 acgaattaat tattacttcg gctatatacc tctaaatttt acatcagttc aattcgacct
     1861 caaatattca tcggcatcct tatcaaaaaa gaaacttaca tccatattct tggcgaaata
     1921 attgcgcata tcgcgggagt tggtgaatgt ttcatcaata ttatcagtca ccggatcaag
     1981 cttaaaacgg tcattacctg tctgcgtgaa aagataaaag taatacgtag gtgttgtact
     2041 tccggtttcg taaatattca aaaatttctt tccattaata tccgacaaaa atccctcata
     2101 gactgtctcg gaaccatcgg aggtgctttt tttcattatg ctgtacacaa aatcgttctt
     2161 cgctgttatg acataggtat aatcagaact gctcctcaac tcccactttc caagcatagc
     2221 cttgtcaacc ggaatatttg gtcgctcatc aagggctatt tcagcgctgt atgggcatcc
     2281 agcaaacaag attcctgtaa taaggaacat cactatataa agacttcttt tcattttggt
     2341 tgatttttaa caaatatagg cattcaggca atatttttac aggcttatcg tcaccgctat
     2401 tatgatatgg acagcaatgg gcgccactcc ttcaggagaa tcgcttagaa gaatagcaag
     2461 catgccgaac tataccaaag gtcagtttca aaatattgaa catacaccgg taatgtcgga
     2521 agactaccat atgggtaaag tgatgatgga tatgatgaag cgaaattctg cccgcacacc
     2581 tgcaacgcct ctccctaacg taaaaaccga tctgcttcat cttgaaaata atgcattggt
     2641 ttggttcggg cactcagcat atctgctcaa aatcaatgga aagcatattt tggtcgatcc
     2701 ggtttttagc ggacatgcat ctccggttcc ttttatgatt aaagcgttca aaggatctaa
     2761 ctcctatggt gttgcggaca tgcctgaaat tgatatgctc gtaatcactc atgatcatta
     2821 tgaccatctg gattatgaaa cggttgttgc actcaagtca aaggtgaaag aagttgtatg
     2881 ctcacttggc gttgctgcgc atttgattaa atggggatat caggaatcgc acattcatga
     2941 gttgtattgg catgaatcga aaacggtttt tgaaaatttc acatttaccg cagaacctgc
     3001 ccgacatttt tcaggacgtg gaataactcg aaataaatcg ctatggtgcg cctttgcttt
     3061 gcagactccg gagcttactg tctttattgg tggcgatagt ggatatggaa aacattttaa
     3121 agaaattgga acgcgatata atggttttga tatcgcatta ttagaatgcg gacaatacaa
     3181 tcctgcatgg aaatatattc atatgatgcc tgaagagaca gtactggctg cgcaggatat
     3241 tcaggctaaa atatttgttc cggtacattg ggggaaattt gccttgagtc tacatacatg
     3301 gcaggaaccg gccgacaggg tagtggctgc tgcacagcga gcagaaatgc catacgctac
     3361 gcctaaaatt ggtgaagctt atgtaattgg tgccggtggg caaacgcatc actggtggca
     3421 aatgcgctaa gttgcggggt gtgattgtgg tgtgtttttt tcacgcagag gcgcagagtc
     3481 gcagagggcg cagggaggcg gaggggagcg gatgatttat tcttgggttt aggattaaag
     3541 ttaattttat aattttctcc gtgtaaagct gtgctcacct atgtgccctc cgtggttaaa
     3601 aaaaacacaa tgcaattaac aacattaatg tcaatggttt attgcaataa ttaatgcgcc
     3661 accgtaaact taaaattctt ctgctcagtt cctgaaacca acactaccaa ataaacaccc
     3721 gaaggccatg aagcaacatc aatgttatgg gtatactgtc cggcatcaac attttggtca
     3781 tttacgagtg gcaacatctc ttgtcctgct aaattgaaaa ttgtcaccaa cagcggtccg
     3841 gattgtttta aactatattt gacttctgcc tggctaactg tagggtttgg atatacacca
     3901 agtgataatg ttctattggt caatagactt tccgattctt ttatgggctg caaatctgtt
     3961 gtatcggttt ctattgaaat aacgttgact atcttagtgt agcttaatcc acctgcaaaa
     4021 aaacattgag tcttcgaatt tgatggaaca cttacattta ccgtttcaag ttcaatctcg
     4081 gtagtatccg taatttctga aatgaatgtc tgttgttcat ccaatattgt ttgcttcaca
     4141 gcagtatatt cagggttttg tatggcaatt tcttcggttg cggtatccat gctatttatt
     4201 gcctcattct caaaaaattc ctgctgagca atttccttta tttgatgatg ttgctcttcg
     4261 gtgcatgaaa ataatagcgg accaaaaact accaacagtg caaatgcaaa tcgcattgaa
     4321 aaactttttg acggcttaat atcatctttt actgtaactg acatgcgcat cgcattagac
     4381 tgtactcttc cgcacgcccc cggatgttgt tgaataaatg taatcaactg ttccgtattc
     4441 aatgcggaga ggtcatgcac taccttatta caggaatcgc aaaatgcacc ggattggtgt
     4501 ttatgcatct tgttgaaatc ttcactacat gcagtaatga cgggttggat aacggtaagg
     4561 gtgatcatag tggagaagtt tataattcag caagatagca tctttgccgc cccaataagt
     4621 cactaaaatg tcaagctgct tttattgaca ctaatcttac aaattagtaa acgtatacaa
     4681 ccgtgtttaa agtgtgtaac cgagcgaaat agtcgggagt ataaacaagt tggagagggt
     4741 ctccggtctg tcgttatccg atgattgttg gttgatataa aatacactgc gcacctgcgg
     4801 atttacatat aatcgctggt tccaaacata aacctcataa ccaatatagg gtgtaacaac
     4861 aaaagtgctt aatcgtactg ttttcggata gggcgcttgt gtgatatcga aatattcata
     4921 ccccgcaatt gcaccaacat atggagcgct agcaccattt ccaatcagga agcgatatcg
     4981 ataaacgagg gccgcttcaa atcccaaacg tacatctgca gtatcactca cttcatcgaa
     5041 catacccttg tgtgtccaat cgggtacatc taaagatgaa aaatacagac ctcctatcat
     5101 ggtgccgata taattcaaat cgtataatgc ctgaacgcca taaccacgat aaatgaatcc
     5161 tgaaggctcc aattctatgc gaaaaggatg atgctccgta tcgtcgcttt gagcatatgt
     5221 aaaagcactt agcgtcatag ccagagctaa cagtaacttc ttcatggcat gttgggtttg
     5281 attatagcca aagttaacat attaatgctg caaatggaag ggtgaatgtc aattgcctgc
     5341 aagacccaat ccttctaccg cctggcgata tttacttcta tattcaatag agaggcgatg
     5401 atccttaatg aggtcggact tacaagcctc aatcaacgcg gtgcctgtgg tttcaggttt
     5461 gagtttgttt tcaatttcaa attcaacaat gctgattgcg cggtcattcg gcgtaagcaa
     5521 cgaaataaac tgagcttcat cataagatcc ttcagcaacc caaatatatt caggatgctc
     5581 attaaaaaat atcggaacct cctcttgaga aagaggctga acaattaaat ccttttcgtt
     5641 gagactgtca atggagatat acaggtgaca tctgctgcga tattcacttc caaatgtaac
     5701 tggctgaagt ctgtcgatca gatttttgtc gtcgggaaaa atagcatgta atgtttggaa
     5761 tggggtgatg gtgctttccg atttgaagat catccactga tgcaatagtg caacatcttg
     5821 tttgttcaga tttgttttga tgaaatttgt tgaggtatca actgattgaa ataatctatt
     5881 aaggagagtg gttaatggta gtcgcgtttc accattgctg atggtataga tgtctaaatc
     5941 tgcttcctcc acttccatac gcacagccga aatattggtc atctcgcttt taagttgaat
     6001 tgcatataca atactgtcgg cattaaaata cctactgcga atgcgtaaag aataaccatt
     6061 gttgtataaa tcgaaatcag gggtgtcaaa atagatatcc cggtaaatat aattgtcgtg
     6121 tctatgtaca gagagactgc ccagttgccc atcgaaaaca atcagttcgc caacaaggtc
     6181 aatgtctgtg ccggtgagtt tactctgctc ttcggtagcc tgacaaaaga tataataggg
     6241 gagcaggcat atcatcaata ggtaaacacg tttaattccc ataattccgc atcctccctt
     6301 catgctttag aaaatgttca ggatggtcgg caggttgaat ttctttcagc aataaaccat
     6361 tttcaatgag gtactcacca ttttccttta cactataata aatatcgtat tgaataggat
     6421 gaggatcttt gatatgaaat gtatttaata tattttctct tcgcgaagca agtttatagt
     6481 aataaagtgt agcgagaggc tccggccaat gtgcatcgtc gaccattttt cgaagtgcta
     6541 caccatctat ctgtgcaatc aaatttgcca tccacaaaag gtcggcgtat tgcgcatctt
     6601 tgtatggcga taataatgca ttcatcggtt cattaaaata aatgcgattg ccataggtgc
     6661 gcaatacttc ccacccaaaa ttatttaccg ctcctgtttt aaaatcagga ggaactacat
     6721 gtaagtccac tcccatgctt gtacccaaat cagaaaagac tgcagaaatc cgatattgat
     6781 aattgcccat atgtacctga gtgagcaaag tattcgattc gcgcgtatcc caattaccta
     6841 tccatgcatg tgccagcaat gcacctcgca gggcacggcg ttggtcgtta tgcaagttat
     6901 tgggaatgaa tgatcctatg cgtttaaccc tgtcgggacg agcctccaat gcacattcga
     6961 taaaggtcat ataggtgcaa tttgcatatg gtaataaatt attgtcatca ttgatcattg
     7021 catcatcaat cacgccaatt gtactaatga atggcgatag atcaacatga taaatttgct
     7081 tgatcatgtt gatcaaatct tcactcgttt gtaccgcttg ccattttggg aatacgagca
     7141 tggcagcatc gcgaccgtag taataaggat gatctacatc gtagcccaac gctgcaaaaa
     7201 tgcgcgagcc tgcaacatca gtatgaatct catcacccca tttcattacc catccattgt
     7261 ccatgtcgag gtctaaggtg ttgattttag gagctgatcc gtcgtagctg aagtcgtcaa
     7321 acacaaccac catctttttt ttggctttta tttttttcaa tttcgccagt tgatcaaagc
     7381 gtttattcaa tgcaccggca gaatctgcag ggtgccagaa tggtgaggtt ttggcagttg
     7441 gtgttttgtt tatttggtta tctatagcta ctgaaaagac tgatttactg cgttgtgtat
     7501 tcagccacat cgataattga tcgtcaaatc cgatatttgg atgtgcagaa gctaagattt
     7561 tctcttttaa actcgattcg aagagtgcaa tctcatataa tatctcgagc aatgagtcat
     7621 taacaggcgt ttgtgtttcc catgcagttt cgagtgtatt atttaattgt ttgatatgtc
     7681 tgacaaaggc attttttctt cgcggaatac tatctccctt gtagatggta atattcgggt
     7741 cagtaaggaa attacatgtg tttctgattt cccggaggtc gagatagccg tcatcaataa
     7801 atcgtaaaac ctcttttgga tgatcgttaa ggataagact tccgcgtggg atggaaggtt
     7861 ttgatgagtt aatcaacaaa gaagaaaaaa cgacacaaag taaaaatgcc ttcatgctac
     7921 ctgctccatt cctgtttgct gatataaatg atggaaattc caatcaatag cgcccagaat
     7981 gccaatattg gcgaaagcga ttcgattcct gcatattgcg tgaaaaatcc ccacatgaaa
     8041 agcgcggtag tatttgtgag aatactcagc cacatgccaa gatgcaataa tgattcataa
     8101 ggtgtgcagc gattgatatg aaaatgtatt tcgtccattg tttcatgggc agtccgggtg
     8161 ataaatcctg cgatgaaaaa aggcagcagt atcagtggct caaaccaata catcagaaaa
     8221 agtgatgtca tcatgaacaa ggaagtagcc aaaatgatac gatgcgtagt gagttcttgc
     8281 ggagtgatat cactgtgtgc cagtttgcca tgtgtaatgc tgtcgagcca ccagaaacag
     8341 gtaaaactcg ttccggatat cagaataatc agcgcgaatg ccaggggtga tatttcagag
     8401 attgccgaca tacaattgac tgacaataaa catgatgaaa attacagcac aagtgagtaa
     8461 attaatagtg tgtgtccatc ttgcggcatc aggtgatttt ccaattttat aaatattcca
     8521 tgcacgccaa attaccggtg ttaaaaaggc aatggcaata tatatttcag gacatatgca
     8581 aataaacacg gtcaatgaaa gtatattcca gatttgtgca taaaataaag ttttcgatac
     8641 accccatcgc accggtaatg tttgcacttc aaatagtttg tcatgcactc tgtcggcaat
     8701 atcgcgcagc acactaccca tggtaacctg catgatagca aaccatgtaa ggccaagtat
     8761 caatggtgtt gtgatatgat cggcaccaag agacgggagc agtccccata gcacaccaat
     8821 gctgatattc tttagtaaaa aagtatgttt aatacgcaac acccgacctc taaaatgcag
     8881 aggaacagaa taaagtatac caatcacacc tataatcgac aaaagtgtaa gtcggaaggg
     8941 tgataaataa atccaggcca gtggagtagc tacacaaatt aattgtacta taaacagcaa
     9001 atgtattggg gatcctgtaa tctttttact gttgaagcga aagccatggg catggtcgat
     9061 aatgtcattg aagcgataaa tcatgaaaac tccgcaaagc acaaaccagg tggatatgct
     9121 caggtcaatt attgattgct cgggtgaaat gataacacca caaaaaaata gcgacaatgc
     9181 cgcggcatgg aaataatgaa gctttcggta gctgacatga ttcaactcca gcaccttagg
     9241 acctaataca gaggtgttgt gtggtaagga aggggagata tctggctgga tgaacataaa
     9301 gtgctaccgt aaagatactt tggagggcgg ataggatgta tgaaaatttc ataaaaagga
     9361 agcaggcatt tgcgaaaatc aataaaatag aaaacgctgc accggctccg gactgtgaaa
     9421 cattttgatt gaccttttgg gtttcatctt cgcagcagac atgaggagcg gacatggcgg
     9481 ggcggtgtgg agcatgagcg gcgattgaat ggtttggcag tcggcagcat gcgtatatga
     9541 tgtattctta caaggcgttt atgtggaaaa ctgctgcagt ttaaggtcaa ttcaacctgc
     9601 caaagaccct gatagattcg attgacaatg gaaacttggt ggtattctga gattttgaaa
     9661 agtcttcaga ataaagggtt tttgcgaaat agaacacgga ttcgcaggaa tagtcgagtg
     9721 cagtaggcgg gtggattgat aaaagtcatt ggcttaaaag ggctacatgt ttgcagattt
     9781 cagcggttta agggtcaaaa gccatatctt tgcggtaata ttcgtttaac agccaattaa
     9841 tctgatgaat aaaaagggct ctgtaaagtt tgtaaatccg gatcagcatc tgttttatca
     9901 aacattgaaa gaaaacgtaa atcgttattt taaggaaaac ggaatttcca cacatgctaa
     9961 tgcgcagatg gttactaaaa ccatcgtgtt attcatcatg tatttagcgc catatgtatt
    10021 gcttatgacc actccaatgt catacggact ggcattagga ttatggagtt taatgggtgt
    10081 ggcgtttgcc ggtatcggta tgagtgtgat gcacgatgcc aatcatggag cttatagcga
    10141 tagtcccggt gtaaataaaa tgatgggtta ctcgttgaat atggtgggta gcatggtatt
    10201 caattggaag ttacaacata atattcttca ccatacttat accaatatca ccgggctgga
    10261 tgaagacata caagcaaagg cgggaatgcg tttcacgcct aatgtgccat taaataagcc
    10321 acatcgctgg cagtttattt atgtatttgg attgtatggc atcatgactt tgtattggat
    10381 cattttcaaa gacttttttc agttagtgcg atatcgcaaa cagggtgtaa acgctgcatc
    10441 atcgagagaa taccgcatgc agtttattaa attgctggct ataaaagcga tatatttttt
    10501 cgccatcatt gcagcaccca tcatttgggc aggtttcagt ttctggcagg ttgctctggg
    10561 atttgtagtg atgcatgtgg ttgggagttt cattttgtcg ctcgtgtttc aattggcgca
    10621 taccgttgag ggtactgatc acccaacacc tgataaagat ggcatcgttc ataatatctg
    10681 ggccgtgcat cagttgcaga caaccatgaa ttttgcgcgc aaaaacaagg tccttagctg
    10741 gtatgtaggg ggattgaatt ttcaggtaga acaccatcta ttccccaaga tttgccatgt
    10801 gcattacccc aaaatagccc ctattgtgaa gcaaacggca gagcagttca atattcctta
    10861 tctcgagaat gaaacctttt ggattgcact gagatcccat cttcgcctgc tttacgacct
    10921 gggacgcatc cctaaattgg atgatatcat ggattaaagg ggtaggggct tgttttataa
    10981 gttgggatgt tatacctttg acctaatctt gggctatctt cttcctttta ccggattctt
    11041 cggttaagtt aagcgggcac tcccggagta ttttgttaac atttttttaa aagaaaaaca
    11101 tgaatttgta cgtttccggt ttgaatttca aactgaccag cgatgaactg cgcaccttgt
    11161 tc
//
LOCUS       DAOQHY010000110        11108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2918_length_11108_cov_4.753099, whole genome shotgun sequence.
ACCESSION   DAOQHY010000110 DAOQHY010000000
VERSION     DAOQHY010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11108
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2918_length_11108_cov_4.753099"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PLL28_09765"
     CDS             complement(<1..477)
                     /locus_tag="PLL28_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HNF69651.1"
                     /translation="MATAKLSGTAAQELLDILKKRFDKHPQRHKDIKWASVLKKLEAS
                     PKALWSLNEMEMTGGEPDVVGMDPSTGKFIFMDCSPESPSGRRSLCYDDAAHASRKEH
                     KPAGSAMGLAAEMGIELLDETWYRHLQTLGNFDNKTSSWLQTPSPIRKLGGAIFGDF"
     gene            619..1563
                     /locus_tag="PLL28_09770"
     CDS             619..1563
                     /locus_tag="PLL28_09770"
                     /inference="COORDINATES: protein motif:HMM:NF013507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNF69652.1"
                     /translation="MHLHRNRLIAVLLLCSVTTTKAELVWTEKANFGGEARHRAVGFA
                     VGERGYMGLGHYNSIVDILFEDIWEYDPSTNTWAQKADFPGGKMLQCVAFTIGQTAYV
                     GTGRDPMFVEHDEFYAYNPKTNTWTEVAAFEGSNRHGAVAFAINGKGYVVTGSDGVNY
                     KKDCWEYDPLANDWIQKANLPGAGRIAAVGFAIGSYGYVGTGNAFGSTNDFYAYDPIL
                     NTWTAKADVPGPTRQEATGFAMNGKGYIGTGDNYSSGDNYDDFWEYHPDTDTWDSVPE
                     FPGMGRRYMTSFTIKNVGYCGLGTSGTNLKDLWQLKAH"
     gene            1568..2797
                     /locus_tag="PLL28_09775"
     CDS             1568..2797
                     /locus_tag="PLL28_09775"
                     /inference="COORDINATES: protein motif:HMM:NF024807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNF69653.1"
                     /translation="MQKIITTIICLLCAWSLDAQNTWSPVADFAGGERERAVAFTIGG
                     RAYVGTGVDSANVCKKDFWEYDPGSNSWTQKADLPGSQRRDAIAFTIGNRGYVGTGLN
                     GLVAWSGTKKKDFFEYNPITNTWTAKDDFEGNSGQGIYYASAFATTTKGYVICGKFGP
                     SWYSNELWQYDPATDDWVKKANFPAGSRYGVSAFAIGDKGYVGCGADENYFTNDFYEY
                     NEATNTWSEKASFPGSPRFNASAFAIDTRGFIGMGTDGGYQKDLYEYNPATDSWMQKA
                     NFPASERRSCVAFTINGYGYLGTGKGPTGLKRGMYKYKPYFFLQNETDDYRLAAVVDI
                     TGNTPRIIVQQSDVNTSYTLHVFNINGQLVFQNHQSGNGEFAIGDLHANGLFVYEIAA
                     TDINGEIQYTSGKLFTR"
     gene            2810..4066
                     /locus_tag="PLL28_09780"
     CDS             2810..4066
                     /locus_tag="PLL28_09780"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69654.1"
                     /translation="MKKLVTLSIITLISISAVQAQAWEKKADFPGDGRSAASSFSFSD
                     NGAIGAGYDGEDFRRSFYSYDPISDTWTQIESIGGAVGDGMERNCAASFTIGDMGYIG
                     TGQAGDPFLNDFWAYNHTTNTWAPKPSVGGIDRRCAVGFSIGSKGYIALGQDAAGYRK
                     DVWAFDTTTNTWSQKADFAGTPRRLAAAFTIGGLAYVGTGDDGAFTKDFYVYDPTMNI
                     WNARAPFGGSPRYSTVSFAVNGKGYIACGYDTTLVNRDDFWEYDPIADTWTQMPDFPG
                     GARVNATAFVIDTLAFVGMGYDTSFNYDIWLWGDTTNIVPVDTQDAVTDLHALQATVQ
                     LFPNPVTAYANIDIQQMQDGDDLIIEIFDLNGSNVTRDCIVSWSGITSDHYTGKMDVH
                     ALPAGTYQCVVHNGKAHKATKFVVIK"
     gene            4063..4434
                     /locus_tag="PLL28_09785"
     CDS             4063..4434
                     /locus_tag="PLL28_09785"
                     /inference="COORDINATES: protein motif:HMM:NF027575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4907 domain-containing protein"
                     /protein_id="HNF69655.1"
                     /translation="MMQTFRCLPAMLLIVGITACSSNTRPIDETHASKSSPTATSSTQ
                     YTCIPFQVEGGWGYDILIDTVRYVHQEHIPAINGLHPFASESDAYKTGELAIKKLEQG
                     IMPPTITVEELRELGIVITAL"
     gene            complement(4440..4826)
                     /locus_tag="PLL28_09790"
     CDS             complement(4440..4826)
                     /locus_tag="PLL28_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69656.1"
                     /translation="MQTSATTPLLILVAGPYRSGTNDDPALIAANVKAMTDTALELYR
                     LGHMPVLGEWFALPLIEAAGSKQIGDAIFNELFHPVAVKLIDHCDAVLRIGGPSAGAD
                     EMVNTGKAKGKLIFMEMKEIPQLNNK"
     gene            complement(4957..6423)
                     /locus_tag="PLL28_09795"
     CDS             complement(4957..6423)
                     /locus_tag="PLL28_09795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69657.1"
                     /translation="MTFLLITAGFKCFCQDWALFPHGTDMYYFEEGTTTLFSCKQDSI
                     LVAGDTTIYYFNKIAPEGATQACYDSILDYDEYTMFSWPSIKMEFTGVDYNLFFYCDE
                     FGALPAIFKSLAAVGSNWEIPNASDYSEFSTVTITCDSIILGSFLGITDSLKYYSIQT
                     GAPLTGTYTIDKAQYILSKHNGLIRFLPFQKLLNPTTCEDCFKCYDLVGQHSDIGTIG
                     WSGPLWENWIQLSEGDFLKYKHYSNSIFGTTESYTTALIQSVQHFSDSIVIESLNADG
                     LLGTSTYYKAAIFNAINAPVWQPFYIPEYHNQPELDRLIGIADSYETIVDTTTFPGHW
                     RYKQSLVTDAVQDNDFCYVEPSFEYGHEIRWDSYIGWTFESSGGPKYYNYSLLIGYVI
                     DGQTYGNYWPLTAEELAMQKPLLSIFPNPATNTITVNLPLETFSQYEICDITGKICSS
                     GTMQNMSQTIDIGDIPSGTYILKVASDHAIQTGKFVKL"
     gene            complement(7127..7567)
                     /locus_tag="PLL28_09800"
     CDS             complement(7127..7567)
                     /locus_tag="PLL28_09800"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNF69658.1"
                     /translation="MTNEHEIVSSRIISASIDKLYQAFADPEKLAQWWGPKGFTNTIH
                     AFDLRVGGKFELTMHGPDKGNYENASVFTKVIPLNLISWHRITQPWFDMEVIFQDIDT
                     NSSSITFRMIFDSAESCQKLRRFVEPNNEENFDRLEAMLATSGL"
     gene            complement(7571..8239)
                     /locus_tag="PLL28_09805"
     CDS             complement(7571..8239)
                     /locus_tag="PLL28_09805"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HNF69659.1"
                     /translation="MYHFSYFKEKDRATIIDFMHKHPFAFVTGSFHDGRQVATQVPLL
                     IEERNGELVLQGHFMRNTDHHKAFIENPNALIVFTGPSAYVSASWYSNPQIGSTWNYM
                     SVHVTGQMTFMSHEQLIQLMKKLTLHFEKENAASPTIYDNLPDTFLDKMMPAIVGFEV
                     KAQKLENVFKLSQNRDPQSYDNIIMQLEQLGGESMLIAQEMKNRRGELFAEGSEWDPN
                     KFAS"
     gene            complement(8261..9004)
                     /gene="arr"
                     /locus_tag="PLL28_09810"
     CDS             complement(8261..9004)
                     /gene="arr"
                     /locus_tag="PLL28_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase"
                     /protein_id="HNF69660.1"
                     /translation="MEFSPQNAIVRLCLAGMEQETNGDPLAAKTLFMEAWSTSTNDFE
                     KFISAYYVARHQTSPTDNLHWLQLAIEFALSANTLSASSALPNVYAKIASCYKEIGDD
                     AQSSKYRQLSEESAHQLPDKGPFYHGTRADLQTGDDLLPGNRSNYQSDIIMNHIYFTA
                     LLNGAGLAAALAKGEGRERVYIVEPTGDFENDPNVTDKKFPGNPTRSYRTTFPLRIIG
                     EVMEWERQTPEQIQQWKDRLAKNSGEIIN"
     gene            complement(9016..10857)
                     /locus_tag="PLL28_09815"
     CDS             complement(9016..10857)
                     /locus_tag="PLL28_09815"
                     /inference="COORDINATES: protein motif:HMM:NF003780.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HNF69661.1"
                     /translation="MKLNQFKSALSGFLFLLGSTIYAQNGKNGPQPIITNGEAQIVPE
                     YRDRLVQELWVETTFDSDGDGRLDRMHTYVTRPTVTDSFDLKLPVIYTTSPYYGLRLW
                     ALLDLFRGNTYWNVKHELGEQPKPHHHSNLGTRTKRPFMASYMDNLWTTRGFIMVYSS
                     SPGTGLSDGSPTVGGENESLAPKAVIDWLCGRGKAYSTRTGNDEVKAFWCNGKVGMTG
                     TSYDGTLCIAAATTGVQGLEAIIPVAPVTSFYNYYRSNGLVRSPDGYLGEDMDVLYDF
                     INSGDKSKRARNDREVRDSILVKNIDRVTGDYNAFWASRDYLNDIDNMHCAMLMAHGF
                     NDWNVMTEQSYRFYLAAKEKGLPVQLYYHQQDHGGDPPLTMMNRWFTHYLFGENNGVE
                     NDPPVQIVREFEELPTPYAAFPDPDAENVTLYLQGGVNNTGSLIIAANNTLVLDTLVD
                     DYTISGDMLIQEKYSEHRLLFVTPTLKKDIRISGIPSVNIRMASSAQAANLSVWLVAL
                     PWEDEKNTQLYENIINRSWADPQNYRSISKGELLKVGEFYQVNFNLMPDDQVIPQGQQ
                     IGLMIFSSDKDFTIAPKPGTELTIDLSRTNITIPFVGGVEAYEDAVK"
BASE COUNT         3192 a         2266 c         2390 g         3260 t
ORIGIN      
        1 gaaatcacca aaaatagcgc cgcccagctt gcggatagga gagggagttt gcagccagct
       61 ggaggtctta ttgtcgaagt tcccaagggt ttggagatgg cggtaccaag tctcatcaag
      121 cagctcgatt cccatttcgg cggccaatcc catggcgctt cctgccggtt tatgctcctt
      181 gcgagaagca tgagcggcat cgtcgtagca gagacttcgg cgaccggaag gactctctgg
      241 agagcaatcc atgaaaatga actttccggt tgatggatcc atccccacca catcgggttc
      301 accacccgtc atttccattt cgttgagcga ccataaagct tttggcgagg cttcgagctt
      361 tttaagtaca ctcgcccatt tgatgtcttt atgtcgctga ggatgcttgt caaagcgttt
      421 cttcagaatg tccaaaagct cctgagcagc agttccggat agtttcgcag tggccataaa
      481 tgattcgttt aaaggtaaat acctacaacc aaaactaatt aattcttacc aacccctccc
      541 acctcctctc tcctctctcc tctctcctct ctcctaaaat ttacatatat ttaacctgaa
      601 ttaaacacac cctcacaaat gcaccttcac agaaaccggc tgatcgccgt actcctcctg
      661 tgttcggtta ccaccactaa agcagaattg gtatggaccg aaaaggccaa tttcggcgga
      721 gaagctcgtc acagagccgt aggatttgcc gttggagaac gcggatatat gggacttggc
      781 cattacaatt ccattgttga catacttttt gaggatatct gggaatacga tccctcaacc
      841 aatacctggg cgcagaaggc ggatttcccg ggcggaaaaa tgcttcaatg tgtagcattt
      901 accattggtc aaacagctta tgtcggcaca ggacgcgacc cgatgtttgt ggagcatgac
      961 gaattttatg catataatcc caaaacaaac acctggacag aggtagcggc ctttgaaggt
     1021 tcgaacaggc atggtgcagt tgcttttgca atcaacggta aaggttatgt tgttaccggc
     1081 agtgatggtg taaactataa aaaggattgc tgggaatatg atccactggc caatgattgg
     1141 atacaaaaag caaatcttcc cggagccggt cgaattgcgg cagtggggtt tgcaattggc
     1201 agttatggtt atgtgggaac gggtaatgcc ttcggatcta ccaacgattt ctacgcatat
     1261 gatcctatcc ttaatacctg gacagcaaaa gcggatgtgc cgggaccaac acgtcaggaa
     1321 gcaacgggtt ttgcaatgaa cggaaagggt tatatcggca caggagataa ttattcgagt
     1381 ggcgataatt acgacgattt ctgggaatat catccggaca ctgacacttg ggattccgta
     1441 cctgaatttc cgggcatggg tcgacggtat atgacttcat ttacaattaa aaatgttgga
     1501 tactgcggac ttggaacaag tggtaccaac ctcaaagatc tctggcaact aaaagcacat
     1561 taaggtcatg caaaaaatca ttacaactat tatttgcctc ctttgtgcat ggtcattaga
     1621 tgcacaaaat acctggtcgc cggtcgcaga ctttgcaggc ggtgagcgtg aacgcgcagt
     1681 agcatttact attggtggtc gcgcatatgt gggaacaggc gtagattccg ctaatgtatg
     1741 taaaaaggat ttctgggaat acgatccggg ttcaaattca tggacacaaa aagccgacct
     1801 tcccggatca caacgccgtg atgctatcgc atttaccatc ggcaacagag gatatgtagg
     1861 aaccggctta aatggtttgg tagcgtggag cggaactaag aaaaaagact tctttgaata
     1921 taatcccatc accaatacct ggacggcaaa agatgatttt gaaggaaata gcggacaagg
     1981 tatatattat gcgtcagcat ttgctactac cacaaaagga tatgtgatat gcggaaaatt
     2041 cggaccaagt tggtattcaa atgaattgtg gcaatacgat ccggcaactg acgattgggt
     2101 gaaaaaagca aatttccctg ccggctcaag atatggtgta tcggcatttg caattggtga
     2161 caaaggatat gttggttgtg gtgctgatga aaattatttt accaacgatt tttacgaata
     2221 caacgaagcc accaatacat ggtctgaaaa agcatcattc cccggttcac ctcgatttaa
     2281 tgcatctgca tttgcaatag atacacgcgg atttattggc atgggcacag atggtggtta
     2341 tcagaaagat ttatatgaat ataatcctgc taccgattca tggatgcaaa aagcgaattt
     2401 ccctgcatca gaacgcagat catgtgtggc attcaccatc aatggttacg gctatttagg
     2461 aacgggaaaa ggcccgacag gtttgaaacg cgggatgtat aaatacaaac cttatttctt
     2521 ccttcaaaat gaaaccgacg attatcgcct ggctgcagtg gttgatatca ccggcaacac
     2581 accacgtatc attgtacaac aaagcgatgt caatacaagc tatacattac atgtatttaa
     2641 tataaacgga caattagtat ttcaaaacca tcaatccgga aatggcgaat ttgcgatagg
     2701 cgatttgcat gcaaacggac tatttgttta cgaaattgca gccacagata ttaatggtga
     2761 aatacaatat acttcaggaa aattatttac ccgttaactc ctcacaacaa tgaaaaaatt
     2821 agttacgctc agtataatta ctttaatttc aatttccgct gtacaagccc aagcatggga
     2881 aaagaaagct gatttcccgg gcgatggacg ttcagcagca tcaagtttta gtttcagcga
     2941 taatggcgct attggtgcag gttatgatgg agaagatttt agaaggagct tttattccta
     3001 cgatcccatt tctgatacgt ggacacagat tgaaagcatt ggtggtgccg ttggcgatgg
     3061 catggaacgc aattgcgcag catcatttac aatcggagat atgggttaca tcggcacagg
     3121 ccaagcgggt gatccatttt tgaacgattt ctgggcatat aaccatacaa caaatacatg
     3181 ggcgccaaaa ccatctgtag gtggaattga tagaagatgt gcggtcggat tttccatcgg
     3241 atcaaaagga tatattgcat taggtcagga tgctgcagga tatcgcaaag atgtttgggc
     3301 atttgatact actacaaata cctggtcgca gaaagccgat tttgccggaa caccacgtcg
     3361 ccttgcagcg gcattcacca ttggcggact cgcatacgtt ggcacgggtg acgatggcgc
     3421 ttttaccaaa gatttctatg tgtatgatcc aacaatgaat atctggaatg cacgtgcccc
     3481 atttggaggt tcaccgcgat atagtactgt ttcatttgca gtgaatggca aaggatatat
     3541 tgcctgcgga tatgacacca cgctagtaaa tagagatgat ttttgggaat atgatcctat
     3601 tgccgacacc tggacacaga tgcctgattt tccgggcggc gctcgtgtga atgcgacagc
     3661 ttttgtaatt gatacacttg catttgtagg catgggatat gatacttcat ttaattatga
     3721 tatctggttg tggggcgata ccacgaatat tgttccggtt gatacacaag atgctgttac
     3781 cgatttgcat gcattgcagg caacagtgca attattcccg aatccggtta ctgcatatgc
     3841 caatattgat atacaacaga tgcaggatgg cgatgattta ataattgaaa tattcgattt
     3901 gaatggaagt aatgtgacac gcgattgcat tgtttcatgg tcgggaatta cttcagacca
     3961 ctatacggga aaaatggatg tgcatgcact ccctgcaggt acgtatcagt gtgtagtgca
     4021 taatggcaaa gcacacaagg ccacaaaatt tgttgtgata aaatgatgca aacatttcgt
     4081 tgcctaccgg cgatgttact gatagtcggg ataacagcat gtagttctaa tacgaggccg
     4141 attgatgaaa cgcatgcttc caaatcgtca cctactgcaa cctcttcaac acaatacact
     4201 tgtattccat ttcaggtaga gggtggatgg ggttatgata tcctgatcga cactgttcgc
     4261 tacgtgcatc aggagcatat tcctgctatt aatggtctgc atccatttgc atcggagagt
     4321 gatgcctaca agacgggaga acttgctatc aaaaaactgg aacagggtat aatgccacct
     4381 acaattaccg ttgaagagtt gcgtgaatta ggtatcgtga tcactgcatt gtgatcaaat
     4441 catttattgt tgagttgggg aatttccttc atttccataa atatcaactt acctttcgcc
     4501 ttacctgtat taaccatttc atcagcacct gccgatggtc cgccgatgcg taatacagcg
     4561 tcgcaatggt caattaattt tacagcgaca ggatgaaata attcattgaa aatggcatca
     4621 ccaatttgct tagaacctgc agcttcaatc aagggtaatg caaaccattc gccaagcaca
     4681 ggcatatgac cgagtcggta taattcaagg gccgtatcag tcatcgcttt tacatttgcg
     4741 gcgatcaatg ccggatcatc gttggtgcct gagcgataag gacccgcaac gagaatgagc
     4801 aggggagtgg tagcagaggt ttgcatgcga atgatcaatt gtgaaatatt aacgaaaaaa
     4861 tttgagggtt catattttgt aaatggcttg caagtcaaca tgcataagcc acctaattaa
     4921 taattgcgct acccgatgca aaatgatttt acgattttac aatttcacaa actttcctgt
     4981 ttggatggca tgatcggagg cgacctttag aatgtatgtg cctgaaggaa tatctccaat
     5041 atcgattgtt tgtgacatgt tttgcatagt cccgctagaa catatcttgc cggtgatatc
     5101 acatatttcg tattgcgaaa atgtttcaag cggtaaattg accgtgattg tattagttgc
     5161 cggattaggg aatattgaga gcaagggttt ctgcatagcc agttcttcag ctgtaagtgg
     5221 ccagtaattt ccataagttt gaccgtcaat tacatatcct attaaaagag agtagttata
     5281 gtattttgga ccaccgctcg attcgaaagt ccatccaata taggaatccc atctgatttc
     5341 atggccgtat tcaaacgaag gttcaacata acagaaatca ttatcctgta cggcatcagt
     5401 taccaaactc tgcttgtaac gccaatgacc cggaaacgta gtggtatcga caattgtttc
     5461 atatgaatct gctattccaa taaggcgatc caactccggt tgattgtgat attcaggaat
     5521 ataaaatggt tgccaaacag gagcattaat ggcattaaat attgcagctt tataatatgt
     5581 gcttgtgcct aacaatccat ctgcgtttaa tgactcgatt acaattgaat cactaaagtg
     5641 ttgtacgctt tgtatcaaag cagttgtata cgactctgtt gtgccaaaga tagaatttga
     5701 atagtgtttg tatttaagaa aatcgccttc agacaactgt atccaattct cccataaagg
     5761 accgctccat ccaattgttc cgatatctga atgttgacct accaaatcat aacatttaaa
     5821 gcaatcttcg catgtagttg gatttagtag cttttgaaag ggcagaaatc ttataagtcc
     5881 attatgctta ctgaggatgt attgcgcctt atcaatggta taggtgcctg ttaagggcgc
     5941 accggtttgt atagaataat atttaagact gtcagtgatc cctagaaaac tacccaagat
     6001 aatcgaatcg caagttattg tgacagttga aaactcacta taatctgagg cattaggtat
     6061 ttcccagttt gacccaacag cagcaagaga tttgaatatt gccggcaatg ccccgaattc
     6121 atcacagtaa aagaataagt tgtaatcaac tcctgtaaat tccattttaa ttgaaggcca
     6181 actaaacata gtatattcgt cataatccaa aattgaatca tagcaagcct gagtagctcc
     6241 ttcgggggca attttattga agtaatagat agttgtatct cctgctacta aaatggagtc
     6301 ctgtttgcaa ctgaaaaggg tagtggtgcc ttcctcgaaa taatacatgt ctgtgccgtg
     6361 cggaaacaac gcccagtctt gacaaaaaca cttgaatccg gctgtgatta ataggaatgt
     6421 cattaaagtt ttcatagggt ttcggataat tgagttttga tcacttctta gaaggttatc
     6481 gcgggactat tttcggtatg aagttataaa aatatattga gattggatag gtataatatt
     6541 taaattaaaa tgatatgtaa ataggtttaa gtggtttgtg tttgtatcgc gcacagtcgc
     6601 aaaggtttga aggcgcgaag ctgtggtttg ttgttatggc acgttgcttt cacgcagagg
     6661 cgctgagccg cagagacagc gagtaagttg gttttgaagg gaaagtaaaa taaatgatag
     6721 atcaatctaa gcaaccttgc gaatatcatc gactacttat cagagataat atatgccaaa
     6781 accacagcat tacgatgtca tgaacaatct tagttccaac gatgagggat tacaaggcga
     6841 tgacaacagt ctctacgcac tgtgtttaac tgtaaatcga gttgctaaac gacaggtaag
     6901 ccggaaggca gtcgttaatt actaaaaatt aaatcgctac tcttaattag ataaagaaat
     6961 tagctcatta ctttgtaaaa tccgaccttt tttgggccat aacgcaagca ttagggcggt
     7021 gtcattcatc tgcggaagcc tcgagctttt ggagccacct tttggattca aaaggtggaa
     7081 gtaaagtctg ttacagagcg aactccgagt tcaaacgatg atcaacttat aaaccggaag
     7141 tcgctaacat tgcttccagt ctatcaaaat tctcttcatt attgggttca acaaagcggc
     7201 ggagtttttg gcaagattct gctgagtcga aaatcatgcg aaatgttatg cttgatgaat
     7261 tggtgtcaat atcttgaaag attacttcca tgtcaaacca cggttgggtg atgcggtgcc
     7321 aggaaattag gtttaaagga attactttag taaatacaga tgcgttctca taattccctt
     7381 tatcagggcc atgcatcgtt agttcaaatt tgcctcctac gcgcaaatca aaggcatgaa
     7441 tggtattcgt aaatcctttc ggtccccacc attgggctaa tttttccgga tcggcaaaag
     7501 cttgatatag tttatcaatg gaagcagaaa tgatcctgct gcttactatt tcatgttcat
     7561 ttgtcataac ttacgaagca aatttatttg gatcccattc gctgccttca gcaaataatt
     7621 caccgcgtcg atttttcatt tcctgagcga ttaacatact ttcaccaccg agttgttcaa
     7681 gctgcataat gatgttatca tagctctgcg gatctctgtt ttgactaagt ttaaatacat
     7741 tctctaattt ttgagctttc acttcaaagc caacgatggc gggcatcatt ttatccaaaa
     7801 atgtatccgg caggttatca taaatggttg gcgaagcagc attctctttt tcaaaatgta
     7861 aggttaattt cttcatgagt tgaataagct gctcgtgcga cataaatgtc atctgccctg
     7921 taacatgcac actcatgtaa ttccaggtag aaccaatttg cggattggaa taccaacttg
     7981 cacttacata ggcacttggt ccggtaaata caattaaggc attcggattt tcaataaatg
     8041 ctttatgatg atcggtattg cgcataaaat ggccttgcaa taccaattca ccattgcgct
     8101 cttctatcaa taacggaact tgcgtagcaa cctgtctgcc atcatgaaaa cttccggtca
     8161 cgaatgcgaa tggatgttta tgcatgaagt cgataatagt cgcccggtcc ttttctttaa
     8221 aatatgaaaa atgatacatc ttcctttgaa ctatgaattt tcaattaatg atctcacctg
     8281 aattttttgc aagcctgtct ttccattgtt gtatctgctc cggtgtttgt ctttcccatt
     8341 ccattacttc gccaattatc ctcaatggaa atgtcgtgcg ataggagcgg gtagggttgc
     8401 cgggaaattt tttatcggta acattcgggt cgttctcgaa atcaccggtg ggttcaacaa
     8461 tataaacacg ttccctgcct tcgccttttg ccaatgctgc cgctagtccc gctccattga
     8521 gtaaagctgt gaaataaata tgattcatga taatgtccga ctgataattg gacctgttac
     8581 cgggtaacaa atcatcaccg gtttgcaaat ctgcacgtgt gccatgataa aatggacctt
     8641 tgtcaggaag ttgatgtgcg ctttcttcac taagctgcct gtattttgaa gactgtgcat
     8701 catcgccgat ttccttataa caggatgcga ttttagcata tacattcggc aatgcacttg
     8761 atgcagagag ggtattcgct gatagtgcaa attcaatcgc caattgcagc caatggagat
     8821 tatctgtagg cgaagtttga tgacgcgcta cgtaatatgc cgagatgaat ttttcgaaat
     8881 catttgtcga tgtactccag gcctccataa ataaggtctt tgcagctagg ggatctccgt
     8941 tggtttcttg ttccatcccg gctaggcaaa gtcgaacgat agcattttgc ggactaaatt
     9001 ccatgttgtc gttttttatt tcacagcatc ttcataagct tcaactccac caacaaaagg
     9061 aatagtgata ttagttctgc taagatcaat cgtcagttct gttcccggtt ttggtgctat
     9121 ggtgaaatct ttgtctgatg aaaaaatcat taatccgatt tgctgtcctt gaggaattac
     9181 ctgatcatca ggcattaaat tgaaattcac ctgatagaat tcgccaacct tcaataattc
     9241 accctttgaa atggatctat aattttgtgg gtcggcccag gatctgttga tgatattttc
     9301 ataaagttgt gtgttcttct catcttccca aggtaatgct acaagccata cggataggtt
     9361 tgctgcctgt gcactacttg ccatgcgtat atttacactt gggatacctg aaatgcggat
     9421 atctttcttt agtgttggtg taacaaacaa caatcgatgc tcgctgtatt tttcctgtat
     9481 aagcatatcg ccgcttattg tataatcatc aacaagtgtg tcgagcacca gggtgttatt
     9541 tgcagctata attaaactgc cggtattgtt aacgccgcct tgtaaataca aggttacatt
     9601 ttcagcatcg ggatctggaa aagccgcata aggtgtgggc aattcttcaa attctctgac
     9661 tatctgaacc gggggatcat tttcgacacc gttattttct ccgaatagat aatgtgtaaa
     9721 ccatctgttc atcatggtta acggtggatc gccaccatga tcttgttggt gataatataa
     9781 ttgaacgggc aacccttttt ctttcgctgc aaggtagaat cggtagctct gctctgtcat
     9841 tacattccag tcgttaaacc catgtgccat cagcattgca caatgcatgt tgtcgatatc
     9901 attcagataa tctctcgaag cccagaatgc attataatct cccgtaactc tatcaatatt
     9961 tttcacgaga atactatcac gtacttcgcg atcatttctt gcgcgtttcg acttgtcgcc
    10021 ggaattaatg aaatcgtaca acacatccat gtcctcacct aaataaccat caggcgaacg
    10081 aacaagtccg ttggagcgat aataattgta gaatgatgta acaggtgcaa ctggaatgat
    10141 agcttccaat ccttgaacac cggtagttgc agcagcgatg cataaggtgc catcataaga
    10201 ggtgcctgtc atgcctactt ttccattaca ccaaaatgct tttacttcgt catttcctgt
    10261 gcgtgttgaa tatgcttttc ctcttccgca cagccagtcg atcactgctt taggtgccag
    10321 cgattcattc tctccgccaa cagttggaga gccgtcagac agtcccgtac ccggagaaga
    10381 agaatacacc atgataaatc ctcgagttgt ccataagttg tccatataag atgccataaa
    10441 cggacgtttt gttctcgtgc cgagattgga atgatgatgt ggttttggct gctcacccaa
    10501 ctcatgtttg acattccaat acgtatttcc tctgaacagg tcgagtaatg cccaaagcct
    10561 caatccataa tatggacttg tagtataaat aacaggtagt ttgaggtcaa atgaatcagt
    10621 aactgttggt ctggttacat aggtatgcat tctatccagg cgaccatcgc catccgaatc
    10681 aaaagtggtt tcaacccaca gttcttgcac aagtcgatcc cggtattccg gaactatttg
    10741 ggcttcgcca tttgtgataa ttggttgtgg accattcttt ccgttttgtg cgtatattgt
    10801 ggatccgagc agaaaaagaa aaccggataa tgctgatttg aattgattta gcttcattgg
    10861 tgaatctggt atattgaatt ataatttgaa attgcttgaa tcattttatt acggtcaata
    10921 atgaaatgct gaaatacgta ttgaatgagt taaattctag gacattgtcc taattatttt
    10981 cttaaatcat cctctcttaa tgtggtaatg caaggcaata gcgccatttg caaaagttct
    11041 agatgaaata agatcaagtg caattttttc ctgaatgtct ttgaataacg gaataccgtt
    11101 tcctatta
//
LOCUS       DAOQHY010000111        11106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2920_length_11106_cov_5.452719, whole genome shotgun sequence.
ACCESSION   DAOQHY010000111 DAOQHY010000000
VERSION     DAOQHY010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 11106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11106
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2920_length_11106_cov_5.452719"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="PLL28_09820"
     CDS             <1..1048
                     /locus_tag="PLL28_09820"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNF69662.1"
                     /translation="QMMRYKLLGRSGLRVSELCLGTMTFGEEWGWGAGFEESSRQFHA
                     FLEAGGNFIDTANRYTEGSSERFLGELVKQSGRRDDLVVATKYALVTHERGLNLAGNH
                     RKNMVQSVEGSLKRLQMDHIDILYLHAWDFTTAAEEVMRALHDLVQQGKIFYVAISDT
                     PAWIVSRANTIAELRGWDPFIALQVEYSLIQRTVERDLLPMAQSMELAVTAWAPMAGG
                     ALTGKYLQENNDPKRLQPNSARLNERSRAIAQEVVNIAHETGHSAAQIALRWVMQQHQ
                     QIIPIVGARNADQLKDSIGATEIALSQEHLQRLQQVSAIELGFPHDFLKGDGVRDILF
                     GGKLGELDIHKRFF"
     gene            1061..2155
                     /locus_tag="PLL28_09825"
     CDS             1061..2155
                     /locus_tag="PLL28_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNF69663.1"
                     /translation="MLGNRFSKYEEKPQTTFDTLFELLQELLVYTSGDVTEALDWLNQ
                     LDRQYQITTPEYGMGDFIQELKDRGYLQDDEQQAGTMRITGKMEQSIRRSSLDQIFGK
                     LKKSQRGNHKTSQTGSGDENTTDSRPFVFGDTHEQIDYNESLKNAYINHGIAGFSMDE
                     SDLVVREREFRAQTSTVLMIDISHSMILYGEDRITPAKKVAMALSELINTKYPKDTLD
                     VIVFGNDAWQIEIKDIPYLQVGPYHTNTVAGLELAMDILRRRKNANKQIFMITDGKPT
                     CIMRNGKYYKNSFGLDRIIVNKTLNLAAHCRKLGIRITTFMIARDSYLQQFVEEFTEI
                     NQGKAFYTSLKGLGDYIFEDYERNRRRNVR"
     gene            2177..3712
                     /locus_tag="PLL28_09830"
     CDS             2177..3712
                     /locus_tag="PLL28_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNF69664.1"
                     /translation="MQQQIKTLGELKASGYTAVSVKEEMRRNLIARLKSGKPAFEGIH
                     GFEHTVIPDLERAILSRHNIILLGLRGQAKTRIARLMTSLLDTYIPFVAGSELHDDPL
                     HPISFFARRRIEEMGDDTPIDWLHRDERYTEKLATPDVSIADLIGDVDPIKAANLKLH
                     YNDERVIHFGLIPHAHRGVFVINELPDLQARIQVALFNILQEGDIQIRGFKLRLPLDI
                     QFVFTANPEDYTNRGAIVTPLKDRIESQILTHYPKDIATSRIITSQEAQIHKEQAGVV
                     TLNDLARDLVEQIAFEARKSEYVDQKSGVSARMTITALENLVSAAERRALVNGESSTH
                     VRVGDFWGLVPSITGKIELVYEGEQEGPYIVALNLIGKAIRAQFANYFPEPEKAKKVG
                     NKKSAGNADPGTGSMYQDIVNWFNDGNTLDILNGTSGRTHKANLQKIPGLKKIVERFH
                     GDKTEEEKLFLMEFLLHGLAEYSLLSKHFLQSGMQFTDLLSGMFSNDSFDALEQFGDP
                     DDQ"
     gene            3858..4244
                     /locus_tag="PLL28_09835"
     CDS             3858..4244
                     /locus_tag="PLL28_09835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69665.1"
                     /translation="MRKLYTFMLLVSATVTGAFATLPSGDGEACVMEESTALSRAYGY
                     FGDDPTISIFPNPVKSYATISFTYENVDRICILNIVGREIKTIVPEAGKDEIKVSLVD
                     LQPGWYFLAAYSQGSTLITKKFLKEE"
     gene            4351..6555
                     /locus_tag="PLL28_09840"
     CDS             4351..6555
                     /locus_tag="PLL28_09840"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HNF69666.1"
                     /translation="MIQRKLHLLALALVISVTSIVRPAFAAVNPDEGMWLANLVRQLN
                     FDYLRQMGLQLSAEEIYNTENASLKDAIVQLTQDGSGFCTAELVSENGLLFTNHHCGY
                     DAIASQSTTEHNYLDDGFWAKSYKEELPIPGLAIRILQDAIDVTDSIIPKVEGLDMAA
                     RRAKVTEIQNRMIAKYAEKGYEAELKSMYYGNQYFIYLYKSYNDVRLVGTPPSSIGKF
                     GGDTDNWMWPRHTGDFSIFRVYADKQNNAAEYSPENVPYKPKKFLTVSLDGYKEGDFT
                     MIMGYPGSTERYLTSFGMNEVITESNPAQIDVFKTVTDVMKQDMDKSEATRIQLASDY
                     ASLMNALKLWETQIDGMERMDAMQIKEDREKAFMKWAKTQGSAKEAMYKEMFENFKNT
                     YSTLSTVNKEFYYKIYSVVIVPSGSFALDFNDIETLFGEEKPSEEVKAATIAGIEASA
                     EEMWKTYNYETEVKKTAAILNMLYTKLPAEKYPQVLKDILAKSKGATPQEKFDNWSKA
                     AFAKSVFTTKEKLEAFLKSPSAKKLKKDPLYNYYIALYTDAQTAGTTFRASNTEINRL
                     EREYVAGILEQNAGKVFYPDANFTLRLTYGTVQDYIARDAVFYHWQTTLDGIMEKRDN
                     TNEEFVVPDKMYELWKNKDFGRYADKNGTVPVCFISNLDITGGNSGSPIMNGKGELIG
                     LAFDGNYEGTPGDYIFDPSMNRTISVDIRYVLFVIDKYAGASNLINELKIAR"
     gene            6663..7076
                     /locus_tag="PLL28_09845"
     CDS             6663..7076
                     /locus_tag="PLL28_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HNF69667.1"
                     /translation="MKRNATAVWNGSGKEGAGTMSTQSGVLNNNQYSWSTRFADGIGT
                     NPEELVAAAHAGCFSMKLSFVLGAANLTPDKIETTCTITLDNGAITNSHLVVKATVPG
                     ATAESFAAAAAEAKANCPISKLLNTEITMEATLEG"
     gene            7094..7705
                     /locus_tag="PLL28_09850"
     CDS             7094..7705
                     /locus_tag="PLL28_09850"
                     /inference="COORDINATES: protein motif:HMM:NF015747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNF69668.1"
                     /translation="MHITILGAGNIGLAAGKKWKDKGHSITFGVRDPESQKAWDARNI
                     GAEVKPFQEAMQGAEVVLIAVPFGVTDEVIKNIQFDWDNVIIIDATNPISASTEPFDS
                     AAEAIAAWTGNGNVVKAFNTTGVGNMLNPIYNGTAIETFICGDDADAKKCVAGLAEDL
                     GFRVMDMGGLQNAVLLENLAKMWVTAAYKLGKGPNFAFTVVQR"
     gene            complement(7755..8720)
                     /locus_tag="PLL28_09855"
     CDS             complement(7755..8720)
                     /locus_tag="PLL28_09855"
                     /inference="COORDINATES: protein motif:HMM:NF015213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HNF69669.1"
                     /translation="MNEFIIAFRECLEAALIVGIIYVVLLKNNAKSQIKMVWFSVAAA
                     VTASAIMGVGLYEVLESAGDAFKEFLEGIFMYITAGLLFYVIFWMSKNLASREAITNQ
                     TKATLSQGSRWGIFFLVFFAIVREGFETALFLVASTGIDKSFSYIGFLTGVALAVLIG
                     YLIVVQGKRIQLKPFFKWTSLLLVVFAAGMIAYGTHEIHEYMEYREHEEHASAEATET
                     VNEECHELLEHAVGAESAESEAEEEEEYVFDIFKNKIGNEPLGGMYIYDEDCGVNVHM
                     LNANGLIGSFLKGLFGYNTSPTLMEIILWLLSLGFGIFIWRRAYK"
     gene            8909..10507
                     /locus_tag="PLL28_09860"
     CDS             8909..10507
                     /locus_tag="PLL28_09860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNF69670.1"
                     /translation="MNKYRFLIPIGFAVALAVGVMIGVKISPFAGAAKVYGGASQNNE
                     RLNEILGYISQRYVDSVNVDSIMDAFVSDYLNDPATIEELFKQLDPHSSYIPKEDLQG
                     FNEDLDGNFDGIGIEFNIVDDTIMVVAAMSGGPSNKLGIQAGDKIISIDDSVVAGIGV
                     ASENVIKKLRGKKGTSVKVNIKRQGEKSLLPFTIIRDVIPVTSIDASFMLDAHTGFIR
                     VNKFAEQTPIEFKEALALLVNEGMQNLILDLRANPGGYLTGAVSIADELLPGTPLIVY
                     TNGRSVGRNDYYAGKNGLFEQGKVVVLINEYSASASEILAGALQDNKRATIVGRRSFG
                     KGLVQQVYDLPDSSAIRLTVARYYTPNGRCIQRPYDKGVDAYYDDYMEILMNGGEIPD
                     SLKSLKDVDWGIHPDISVPLDTTPENQTLNYLLNRSLIQQYCYSAYSANPGSFNKNKD
                     MLAFAKTYQITPEMFNKFMQFVHTHDTEKGVTDAEINAAKAKVEIAIKAYFARQLWGD
                     NGYYCTMSMIDDTLTKAINTIEKK"
     gene            complement(10578..10820)
                     /locus_tag="PLL28_09865"
     CDS             complement(10578..10820)
                     /locus_tag="PLL28_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69671.1"
                     /translation="MKKVTYFAFALALTVLVSCKGKSTAEEGDTTTTETTTVETGASE
                     NVDNAAQVDEVLNQSSDDELFGTDVDDAAVEGATTK"
     gene            complement(10872..>11106)
                     /locus_tag="PLL28_09870"
     CDS             complement(10872..>11106)
                     /locus_tag="PLL28_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNF69672.1"
                     /translation="LNEVMQEALEKYHPPAVRGNYIQIKYVTQMPAQVPTFVFFTNYP
                     NDIREPYRNYLENQLREHFPLSGVPINIFFRKK"
BASE COUNT         3418 a         2228 c         2471 g         2989 t
ORIGIN      
        1 gcagatgatg agatataaac ttttaggtag aagcggtctt cgcgtttctg aactctgcct
       61 gggcaccatg acttttggtg aagaatgggg ctggggagcg ggatttgagg agagcagccg
      121 ccaatttcat gcctttctgg aggcaggagg aaattttatc gacaccgcaa atagatacac
      181 cgaaggatcc agtgaacgct ttcttggcga attagtgaaa cagagcggga ggcgcgacga
      241 tttggtggtg gctactaaat atgcacttgt tacccatgaa cgcggtctca acctggcagg
      301 taatcacaga aaaaatatgg tgcaatcagt ggaaggtagc ctcaagcgtt tgcaaatgga
      361 ccatatcgac atactgtatc tgcatgcctg ggatttcact actgctgcgg aagaagtgat
      421 gcgggcgctg catgatctcg ttcaacaagg gaaaatattt tatgttgcca tttcagatac
      481 gcctgcttgg attgtttcgc gtgccaacac cattgctgag ctgagaggat gggatccgtt
      541 tatcgccctg caggtcgaat attcgctcat ccaaagaaca gtagaacgcg accttttacc
      601 aatggctcaa tctatggaac ttgccgttac agcatgggca ccgatggcag gtggtgcact
      661 caccggaaaa tatttacagg aaaataatga ccctaaaaga ttgcagccta acagtgctcg
      721 tttaaatgaa cgcagcagag ctatcgcaca ggaagttgta aatattgcac atgaaaccgg
      781 acatagtgct gcacaaattg cattgcgatg ggtgatgcag caacatcaac aaatcatacc
      841 gatagttggc gcacgaaatg cggatcaatt aaaagatagc atcggtgcaa ctgaaatagc
      901 actttcacag gaacacctgc agcgtcttca acaagtaagc gccattgaat taggattccc
      961 gcacgatttt ttaaaaggtg atggcgtgag ggatatttta ttcggtggaa aattaggtga
     1021 attagacatt cataaaagat ttttctgata tccatttatt atgctaggca atcgcttttc
     1081 aaaatacgaa gaaaaaccac aaactacctt cgatacactg tttgaattat tgcaggagtt
     1141 attggtatat acttcaggcg atgtaactga agcattggat tggttaaatc agctcgacag
     1201 gcaatatcaa atcactacgc ccgaatatgg catgggtgat tttattcagg aattaaagga
     1261 ccgcggatat ttacaagacg acgaacagca ggcaggcacc atgcgcatta ccggcaagat
     1321 ggagcagtca atacgtcgtt catcgctgga tcagattttc ggtaaattaa aaaaatcgca
     1381 acgcggaaat cacaaaactt ctcaaacagg cagtggtgat gagaatacta ctgatagcag
     1441 gccctttgtt tttggcgata cacacgaaca gattgattac aatgaatcgc tgaaaaatgc
     1501 ctatatcaat catggcattg caggtttcag tatggatgag tcagaccttg ttgtgcgtga
     1561 acgagaattt cgcgcgcaaa catcaactgt gctgatgatc gatatatcgc atagtatgat
     1621 tttgtatggc gaagacagga tcactccggc aaaaaaagta gccatggcat tgagcgaact
     1681 catcaacaca aaatatccaa aggatacact tgatgtcatc gttttcggca atgacgcgtg
     1741 gcagatagaa attaaagata ttccctactt gcaggtaggt ccgtatcata caaatacagt
     1801 ggcaggatta gaattggcga tggatatttt gaggcgaaga aaaaatgcca acaaacaaat
     1861 attcatgatc accgatggca aacctacctg cattatgcgg aatgggaaat attataaaaa
     1921 cagcttcggg ctagatcgca tcattgtaaa taaaacactg aatctggcag cacattgcag
     1981 aaaattaggt atccgcatta ccacatttat gattgcgcgc gacagttatt tgcagcagtt
     2041 tgtggaggag tttaccgaga tcaatcaggg caaggcattt tacacaagtt tgaaaggact
     2101 tggcgattat atttttgaag actacgaaag aaacagacgc agaaatgtgc gttgatatta
     2161 atttttgaaa gcaactatgc aacaacagat aaaaacatta ggcgaactca aggcgagtgg
     2221 atatactgcc gttagtgtga aagaagaaat gcgtcgcaat ttaattgcgc gattgaaaag
     2281 tggtaaaccc gcctttgaag gaattcatgg atttgaacat accgtaattc cggatttgga
     2341 acgcgccata ttatcgcgac acaacatcat tttacttgga ttgagagggc aagcaaaaac
     2401 gcgcatcgca cgattgatga catccttgct cgatacctac attccatttg tggcaggcag
     2461 tgaattacat gatgatccac tccatccgat ttcatttttt gcacgccggc gcattgaaga
     2521 aatgggagat gacacgccta tcgattggtt gcatcgcgat gaacgctata ccgaaaaact
     2581 cgccacaccg gatgtaagca tcgccgatct gattggtgat gtagatccta ttaaagcagc
     2641 aaatttaaaa ttgcattaca acgatgagcg cgttattcat ttcggattaa ttcctcatgc
     2701 acatagaggt gtatttgtga ttaatgaatt acctgatttg caagcgcgca ttcaagtagc
     2761 attatttaat attctgcaag aaggcgatat tcaaatcaga ggatttaaat tgcgtttacc
     2821 tctggatatt caatttgtat ttacagccaa tcctgaagat tatacaaatc gcggagccat
     2881 tgttacacct ttgaaagaca ggatagaaag ccagatactc actcattatc ccaaagacat
     2941 agctacttcg cgcatcatca cttcacagga ggcacaaatt cacaaagagc aggcaggagt
     3001 tgttacgctc aacgacctcg cacgcgatct ggttgaacaa atagccttcg aagcaagaaa
     3061 aagtgaatac gtagatcaaa aaagtggcgt aagcgccagg atgaccataa ccgctctcga
     3121 aaatctggta tccgctgctg agcgccgagc tttggtgaat ggagagtcat ctacacatgt
     3181 ccgcgttggc gatttttggg gattggttcc gtcgataacc ggcaaaatag aattagtgta
     3241 tgagggtgaa caggaaggtc cttacattgt agccttgaat ctcatcggaa aagctatcag
     3301 ggcgcagttt gcgaactatt ttccggagcc ggagaaggcc aaaaaggtgg gaaacaagaa
     3361 gtcggcaggc aatgcagacc cgggtaccgg aagcatgtac caggacattg tgaattggtt
     3421 taacgatggc aacaccctcg acatcctgaa tggcacctcg gggcgcactc acaaggccaa
     3481 tttgcagaaa atacccggtt tgaagaaaat tgtagagcgc ttccacggag ataagactga
     3541 agaggaaaag ctatttttga tggagttttt gctccacgga cttgccgagt attccttgct
     3601 gagtaagcat ttcctccaaa gcggcatgca gttcaccgac ctgctgtcag gcatgttcag
     3661 caacgacagt tttgatgcat tagagcagtt tggcgaccct gatgaccaat aggtgaacct
     3721 tttgacctga aatccgtaaa aacgcaatga acaaggtcaa aagtggttaa aatgggttaa
     3781 ttttatcaat atccacatgg cctgattttt gagatttaaa aaaagttata gtatctttac
     3841 ttttagcgtc acagcctatg cgaaaactct acacattcat gttactggtc tctgcgacag
     3901 tcaccggtgc ctttgccaca cttccttcgg gagatggtga agcttgtgta atggaggaaa
     3961 gcacggcatt gtcgagagct tacggctatt tcggcgatga tccgaccatc agtattttcc
     4021 cgaatccggt aaaaagctat gctaccatct cgtttacgta tgaaaacgtt gatcgcatat
     4081 gcattctcaa tattgttggt cgcgagatca aaacgatagt tcctgaagcc gggaaagatg
     4141 agatcaaggt tagcctggtt gatttacagc ccggatggta ttttttggct gcctactcac
     4201 agggaagcac gcttatcacg aagaaatttc tgaaagaaga ataaaagttt gttaaaacga
     4261 agcggctact ggccgctttt tttatgcctt ctgccctttc gtgggcgcag ttccgtaact
     4321 tcgcggtcag aatcatttta acattacagc atgatacaac gtaagcttca tttactggca
     4381 ttggcattgg taataagtgt tacctccatt gtaagacctg cattcgccgc cgtgaatccc
     4441 gatgaaggga tgtggttggc caacctggtt cgccagctca acttcgacta cctccgtcag
     4501 atgggattac aactctcagc agaggagatc tataacaccg aaaacgccag tctgaaagac
     4561 gccattgtgc aattgacaca agatggtagc ggattctgca ctgcagagct cgtttctgaa
     4621 aatggtttgc ttttcactaa tcaccactgt ggttatgatg ctatcgcttc tcaatctaca
     4681 accgagcaca attacctgga tgatggtttt tgggcaaaga gttacaaaga agaattacct
     4741 attcccggat tagcaattcg catattacag gatgcgatag atgttacgga ctccatcata
     4801 cctaaagtgg aaggattaga catggcagca cgtcgtgcca aagtaactga gatccaaaat
     4861 cgcatgattg caaaatatgc tgagaaagga tacgaagcag aattgaaatc catgtattat
     4921 ggcaatcaat atttcatcta tctctacaaa tcatataatg atgttcgcct tgtaggtact
     4981 ccaccttcat ctatcggaaa attcggtggc gatactgaca actggatgtg gccacgtcac
     5041 accggcgatt tcagcatctt ccgtgtgtat gccgataagc aaaataatgc tgcggaatac
     5101 agccctgaaa acgtacctta caaaccgaaa aaattcctca cagtatcact cgatggatac
     5161 aaagaaggcg atttcaccat gattatgggt tatcccggat caactgagcg ttatcttact
     5221 tctttcggta tgaatgaagt aattactgaa agtaatccgg cacagattga tgtatttaaa
     5281 accgttactg atgtgatgaa gcaggatatg gataagagtg aagcaacgcg catccagctt
     5341 gcatctgatt acgcttcatt gatgaatgct ttgaaattgt gggaaacaca gatcgacggt
     5401 atggaacgta tggatgccat gcagatcaag gaagatcgtg aaaaagcttt catgaaatgg
     5461 gcaaaaactc aaggcagtgc aaaagaagcg atgtataaag aaatgtttga aaatttcaaa
     5521 aatacataca gcaccttaag cactgtaaac aaggagtttt actacaagat ttattcggta
     5581 gtaatcgtgc cttcaggaag ctttgcattg gatttcaacg atattgaaac attgtttggt
     5641 gaggaaaaac cttctgaaga agtgaaagct gctaccattg caggaattga agcttcggca
     5701 gaagaaatgt ggaagacata caattatgaa actgaggtta aaaagaccgc agccattttg
     5761 aatatgttgt acactaaatt acctgcagaa aaatatcctc aggtattaaa agatatcctt
     5821 gctaaatcga aaggtgctac tccacaagaa aaattcgaca attggagcaa ggctgcattt
     5881 gccaaatctg ttttcacaac aaaagaaaaa cttgaggcat ttttgaaatc gccaagcgcg
     5941 aagaaattga aaaaagatcc gctgtataat tattatatcg cattgtatac cgatgcacaa
     6001 acagcaggca ctactttccg cgcatcaaat acagagatca accgtttgga acgcgaatat
     6061 gtggcaggaa tactggaaca aaatgcgggc aaagtatttt atcctgatgc caacttcaca
     6121 ttgcgtttga catacggtac cgttcaggat tatattgcac gcgatgccgt attctaccac
     6181 tggcaaacaa cgcttgatgg tatcatggaa aaacgcgata ataccaacga agaatttgtg
     6241 gtgcctgaca aaatgtatga attgtggaag aataaagatt ttggtcgcta tgccgataaa
     6301 aatggtactg ttcctgtatg ttttatctct aacctcgata ttacaggagg taacagcggt
     6361 agccctatca tgaacggcaa aggcgaactg ataggtctag ctttcgatgg caactatgaa
     6421 ggcactcccg gagattatat tttcgatcca agcatgaata gaactattag cgtagacatt
     6481 cgctatgtat tgtttgttat cgacaaatat gccggggcaa gcaatcttat caatgaattg
     6541 aagattgccc gttaatcggg taaaatgata ctgaaaaggc cacaggaaac tgtggctttt
     6601 tttttgtcga taggcacaat gtacactgca actactacct ttgcaataaa taaaacatac
     6661 taatgaaaag gaacgcaacc gccgtttgga acggctcagg aaaagaaggc gccggaacaa
     6721 tgagcactca atccggtgta ttaaataaca atcaatattc atggtcaact cgttttgctg
     6781 atggcattgg cactaatccg gaagaattgg tggcggctgc acatgcagga tgtttcagca
     6841 tgaagttgag ctttgtattg ggagctgcaa atcttactcc cgataaaatc gaaactacct
     6901 gcaccatcac gctcgacaac ggtgctatta ctaacagtca tcttgtagtg aaagcaacag
     6961 ttccgggcgc cactgccgaa tcatttgctg ccgctgctgc cgaggcaaaa gcaaattgcc
     7021 ctatcagcaa attgttgaat accgaaatta caatggaagc cactttagaa ggctaatatt
     7081 aatttttgct gatatgcata ttacaatttt gggagcagga aatatcggtc ttgctgcagg
     7141 taaaaaatgg aaagacaaag ggcattccat cacatttggt gtgcgtgatc ctgaaagtca
     7201 aaaagcatgg gatgcgcgca atatcggcgc cgaagtgaaa ccatttcagg aagccatgca
     7261 aggtgccgaa gtggtactca ttgccgttcc atttggtgtt accgatgagg tgataaaaaa
     7321 tattcagttc gactgggaca atgtcatcat tattgatgca acaaatccaa tctctgcatc
     7381 taccgagcct ttcgattcgg cggctgaagc aattgcagca tggacaggaa atggtaatgt
     7441 ggtgaaagca tttaatacta ccggagtagg taatatgctg aatccgattt acaatggcac
     7501 tgctattgaa acatttattt gtggcgatga tgccgatgca aaaaaatgtg tggccggatt
     7561 agcagaagat cttggtttca gggtaatgga tatgggtgga ttgcaaaatg ccgtattact
     7621 cgagaactta gctaaaatgt gggttaccgc tgcatataaa ttaggcaagg gtcccaactt
     7681 tgcttttacg gtagtacagc gttgaaaaat aattgcgata aaagaaaggc tgccttatga
     7741 gcagcctttc tttattactt gtaagctctt cgccaaataa atattccaaa tcctaaactc
     7801 agcaaccaca aaataatctc cattaatgtg gggcttgtat tataaccgaa taatcctttc
     7861 aggaatgatc ctattaatcc attggcattg agcatgtgca cattcacacc acagtcttca
     7921 tcataaatat acatgccacc aagtggttca tttccaattt tatttttgaa gatgtcgaat
     7981 acatattcct cttcttcctc ggcttcactt tcggcactct ctgcgcctac ggcatgctct
     8041 aataattcgt gacattcttc gtttacagtt tcagtagcct cggcacttgc atgttcttca
     8101 tgttcgcggt attccatgta ttcatgaatt tcatgtgtac cataagctat cataccggca
     8161 gcgaaaacaa cgagtaataa agatgtccat ttaaagaatg gctttaactg aatacgtttt
     8221 ccttgcacca caatcaaata accaatcagc acagccaatg ctactccggt taaaaatccg
     8281 atataggaga aactcttgtc gattccggtg ctggcaacca gaaaaagtgc agtttcaaat
     8341 ccttcgcgta caatggcaaa aaacaccaaa aagaagattc cccatcgcga tccttgcgaa
     8401 agtgtggctt tagtctggtt ggtgatggcc tcccgcgatg ccaggttctt gctcatccag
     8461 aaaatcacat agaatagcag tcccgctgtg atatacatga atattccttc caaaaactcc
     8521 ttaaaagcat cacctgccga ttccaacact tcataaagcc cgacacccat aatggcagaa
     8581 gcggtgacag cagcggccac gctaaaccat accattttga tctgcgattt ggcattgttt
     8641 ttcaatagta ccacatagat gatcccaaca atcagggcag cttccaggca ctctctaaag
     8701 gcaattatga attcgttcat tgaagaaatt tgaaaatttg actatttaga ataattctaa
     8761 agatgtgcaa acttacaaag acagggcgac cgaaggtagg agattggtcg cttagcagca
     8821 taataccgca aatatggtat tcgttaacaa ggtttagtgg tcattacggc ttcggggact
     8881 taactttgca gccacttaaa cctttcaggt gaataaatac agatttctca tacccatcgg
     8941 ttttgctgtt gcgctggcgg tgggcgttat gatcggcgtg aagatatcgc cgtttgcagg
     9001 tgctgccaaa gtatatggag gtgcatcaca gaataatgaa cgactcaatg aaatattggg
     9061 atacatcagt cagcgatatg tcgattccgt gaatgttgat tccatcatgg atgcatttgt
     9121 ttcagactat ttaaatgacc cggctaccat tgaagaatta ttcaagcaat tggatccaca
     9181 cagcagttat attccaaaag aagatctgca aggtttcaat gaagaccttg acgggaattt
     9241 tgatggcatt ggaatagaat tcaatattgt tgacgatacc attatggtgg ttgcagcaat
     9301 gagcggtgga ccatcgaata aattaggtat acaagccggt gataaaatta tttccatcga
     9361 tgattctgtt gtggcaggaa ttggtgttgc cagcgaaaat gtcattaaga agttacgtgg
     9421 gaaaaaaggc acttcggtta aagtcaatat caaacgtcag ggtgaaaaaa gtttattgcc
     9481 gtttacaatc atacgagatg taattccggt tacaagtatt gatgcttcat ttatgttaga
     9541 tgcacatacc ggatttattc gtgtaaataa atttgccgag cagacaccaa ttgaattcaa
     9601 agaagcatta gcattattgg taaacgaagg catgcaaaac ctcatccttg atcttcgtgc
     9661 aaatccggga ggctatctga caggcgccgt ttctattgcc gatgaattat tgccgggaac
     9721 acctcttatt gtgtatacga acggacgcag tgttggacgc aatgattatt atgccggcaa
     9781 aaacggattg ttcgaacaag gtaaggtagt cgtattgatt aatgaatatt ctgcatcagc
     9841 aagtgaaatt cttgcaggtg cattgcaaga caacaaacgc gcaacaattg taggtcgcag
     9901 aagctttgga aaagggctcg tacagcaagt ttacgatctt cccgacagct cggcgatacg
     9961 tcttacggta gctcgatatt atacacctaa tggtcgctgt attcaacgcc cgtacgataa
    10021 aggtgttgat gcctattacg acgactacat ggagatattg atgaatggcg gtgaaattcc
    10081 cgacagtcta aaaagtttga aagatgtaga ttggggaata catcctgata tttcagttcc
    10141 attggatact acacctgaaa atcagacctt gaattattta ttgaaccgtt cactcattca
    10201 gcaatattgc tattcggcat attctgccaa tccgggatca ttcaataaga ataaagatat
    10261 gcttgcgttt gctaaaacat atcagattac gcctgagatg tttaacaagt ttatgcaatt
    10321 tgtacatacc catgatactg aaaaaggagt tacagatgcc gaaataaatg cggctaaagc
    10381 aaaagtagaa atagcgatca aggcatattt tgccagacag ctttggggcg ataatggata
    10441 ctattgcact atgtcgatga tagatgacac cttgacaaaa gcaattaata cgattgagaa
    10501 gaaatgattt caatcataga tataaaaaag cccgtcagtt acaaccgacg ggcttttttg
    10561 tttgatcagc gatcagatta ttttgttgta gctccttcca cggcagcatc atcaacatct
    10621 gttccgaaca gttcatcatc tgatgattgg ttcaacacct catcaacctg agcggcattg
    10681 tctacatttt cagaagcgcc ggtttcaaca gttgttgttt cagttgttgt ggtatctcct
    10741 tcttcagcag ttgattttcc tttgcaagaa acgagtactg tgagtgctag ggcgaaagcg
    10801 aaatacgtta cttttttcat gacagttgtg tttggttatt atagatacaa aattagcaat
    10861 tttcttttcc cttacttctt ccggaaaaaa atattgatcg gcacccccga cagcgggaag
    10921 tgctccctta actgattttc gaggtagttg cgatatggtt cgcggatatc gttcgggtaa
    10981 ttggtaaaga acacgaaggt tggcacctgg gcaggcattt gcgtaacata cttgatctgg
    11041 atatagttac ctctgactgc aggcggatga tacttttcta aagcctcctg cattacttca
    11101 ttcagc
//
LOCUS       DAOQHY010000112        10933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2991_length_10933_cov_4.849513, whole genome shotgun sequence.
ACCESSION   DAOQHY010000112 DAOQHY010000000
VERSION     DAOQHY010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10933
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2991_length_10933_cov_4.849513"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PLL28_09875"
     CDS             <1..410
                     /locus_tag="PLL28_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69673.1"
                     /translation="TTEMLMNSGKESAGIFSVAVIDSKNIVCAGGDYLDPTNTDYNYL
                     LVYIIEGVWDYPIPVSEINKESSGYYSCIDVITNNNITTFFACGTGGVDVWRSTNFKI
                     KVLSMSGFNVLQISPSGKYVFLAGNKVVGKMDI"
     gene            complement(421..963)
                     /locus_tag="PLL28_09880"
     CDS             complement(421..963)
                     /locus_tag="PLL28_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69674.1"
                     /translation="MFQMIRLKNVVALSAICCIGFSGLYAEELVGNQPSSIDKSADYS
                     AGSNTVVYVETDMSTPGTVINADNTFIDAVDAVVKEDTGKKEEQEPVLEPMVLAGTGA
                     AVAGAGVAAAGMFAAPILLAIGALLLIASAILTSKGWKKIKAEPKKYKGEKFAIVNFI
                     IIGVLGVAASFYALYLLFMV"
     gene            complement(985..3204)
                     /locus_tag="PLL28_09885"
     CDS             complement(985..3204)
                     /locus_tag="PLL28_09885"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNF69675.1"
                     /translation="MALINLDGANYYVSPAGNNLNSGIGIASPWKTIQFALNQANDGD
                     VINVMAGTYTGKLTWNDGGVVGQYITLQNYNDDVVILDGSTVGNNQALMYIENKNYIK
                     IDGLRFTNHNGNYQPVINVYGNCSHIEITNCEFYNTNCNESYAILCEGFGDDFLISGN
                     NMHDLIGDNAVGVLFVGSGTTTAYTNIVITNNVLTNLDPAPSEAIAVNGNIDGFTIAD
                     NVLTDINNIGIVMIGGEDWVNTNDAVNQARNGVCRRNAVTAANSIYGGGFSAGVYVDG
                     GKNIVVENNSITDSDIGLEIGCENMGFVCSGITVRNNLIYRNQKSGLGFGGYDYPATG
                     MVTNCAFYGNTVYDNDFLNTGFGQLWIQYATNCIVENNIFFTSTTPWMINAETIDSTY
                     EVNLDYNNYYYSGGPMAMKGFVDFTLITGYEALLSTMGDNAHFSLADPLFVNPAISDF
                     HLQVTSPCINAGNPSYIPVDEFDMDLATRLTGGRIEMGADEQIAAPLSYLVNSIPTIC
                     HGSCDGSLELDGIYGCAPYTYQYKIGAGAWIPFDGFLSDLCAATYKVKVTDACGAFVM
                     GNVVISEDALLTLTVTSIVNETVAGAHNGKITVQSTGGYGSKQYSINGGLTWQTAKKF
                     SGLSAGTYTIMTKDAHNCTASVVATVLVGGRMMDEAESMVYPNPANHEIHISTNQPEM
                     PTIIRLMDLGGKEVFFEETNGGEVLITLPESLPSGLYLLQWSNVLGLFQQQVAVASR"
     gene            complement(3322..4056)
                     /locus_tag="PLL28_09890"
     CDS             complement(3322..4056)
                     /locus_tag="PLL28_09890"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HNF69676.1"
                     /translation="MKTIVHKLLIAILVPFSIASANPPAGWVPVANSTLEIKSQGSYY
                     FSVQLDGRYYPNPVKRFSMATMTAGLHHVDLFAAGQQGHAGAQKLIYSGDIYIDANSL
                     VTGVLDNAGRFYIKSVKSIPVYVPEPQTYYPPNTYPTCGTPPLPPKPLPMAGYSFTQL
                     MDVIEDQWFESGRLQIAKQALNSNWFTANQVAEMMHAMSFEDTRLELAKAAYYKTVDT
                     ENYFIVNKEFWFSSSVDELSRYLSGI"
     gene            4179..6104
                     /gene="dnaG"
                     /locus_tag="PLL28_09895"
     CDS             4179..6104
                     /gene="dnaG"
                     /locus_tag="PLL28_09895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNF69677.1"
                     /translation="MIKKETIDRINDTARIDEVVGDFVRLKKRGVNLIGLCPFHNEKT
                     PSFNVSVPRGIYKCFGCGKGGNAVSFVMEHEKFTYREALLYLANKYNIEVEETERTNE
                     EILEDNRRESLYIINAFAQNYFSEQLLNSEEGNNIGLSYFEERGLRRETIETFKLGYC
                     PSDGDSFTQSAHQAGYQNELLQHLGLTTAYGKDFFRGRVMFTIQSVSGKILGFGGRTL
                     SADKKQPKYINSPETDIYFKSKIVYGIYQARKAIAQHDVCFLVEGYMDVISLHQGGIE
                     NVVASSGTALTQDQVRLIKRYTQNLIILYDGDAAGVKAALRGLDIALEEGMNVKVVLL
                     PAEDDPDTLVQKLGGDGFNKYVEAQKKDLIHLKTDLFLEEAKGDPVKVAGIIKDIVAS
                     IAKIPDAITRSLYVKQTAKMLEVEEQILITETNKLLRKEATKKVTPEDKATVEALDYT
                     PPAKEQPLFTFHLDTPQERDIVRLLLENSNYEIEGENAIARILRNLSDVEIDHPEYNM
                     IIEEYRNAFAQNEFLTQAHFFNHPDEAIRNLTIDLLQSPYELSDNWTKMHDVPITDKQ
                     FLVRKDIVKSISLLKLKKIIRMKQDVDNRIKELQHDTSENGIQETMLCQKESIQLQQF
                     IRKLSTDTGTVVLPGIL"
     gene            complement(6060..6884)
                     /locus_tag="PLL28_09900"
     CDS             complement(6060..6884)
                     /locus_tag="PLL28_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HNF69678.1"
                     /translation="MDKHHHNPFASKPAILLLVLGVFFVVNAIIAEFIGVKVFALEDS
                     LGLKPFNWNLYGQAGSLNFTVGVILWPFVFIMTDIINEYFGVKGVRRLSYLAVICIAY
                     AFAIVYAGISLKPAAFWPGSFVDSGVPDMQAAYAAIFGQGMWIIGASIAAFLFSQIVD
                     VFIFHRIKAFTGERRIWLRATGSTAISQIFDSLIVLYIAFVLGPQHWTLPFFFAVATV
                     NYFYKILAAIVLTPVLYWVHHIIDNFLGTELSEKMRKEAMGISEDAGQNDSAGISG"
     gene            complement(6888..7337)
                     /locus_tag="PLL28_09905"
     CDS             complement(6888..7337)
                     /locus_tag="PLL28_09905"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNF69679.1"
                     /translation="MVKDRILVFIQLVFFAVYIWEPWQYSFQPGIAGRLVGILIGVIG
                     VILVWWPILQLRRNLTMNPTPKTGSHLITTGIFACMRHPIYTGVFLTCVGFAIFQGSI
                     TRMVISILLLLFFDRKTVYEEKKLKLAFSEYEKYAAHTSKFFPFLKK"
     gene            7447..9042
                     /locus_tag="PLL28_09910"
     CDS             7447..9042
                     /locus_tag="PLL28_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNF69680.1"
                     /translation="MRLETEINKRRTFAIISHPDAGKTTLTEKFLLYGGAIQVAGAVK
                     SNKIKKSATSDFMEIERQRGISVATSVMSFEYQGINVNLLDTPGHKDFAEDTYRTLTA
                     VDSVILVIDCVKGVEEQTERLMEVCRMRKTPVIIFINKMDRDGKDPFDLLDELEDKLS
                     IKVRPLSWPINTGTHFKGVYSIYHHKINLFRPGSQRVEDDVITIDNLNDKTLDTQVGE
                     RDAEQLRHDIELIEGVYDAFDTNRYLKADVAPVFFGSAINNFGVQELLETFIQIAPVP
                     QGRDTDKRYVDADEEKFTGFIFKIHANIDPRHRDRIAFLRVCSGHFERNSFYHHVRLD
                     KNLRFNNPYAFLAASKDVVDEAWPGDVIGLYDTGNFKIGDTLTEGEDVMFRGIPSFSP
                     EIFKELVNMDPMKTKQLEKGIEQLTDEGVAQLFVHNNGFKKVVGTVGELQFEVIQYRL
                     EHEYGAKCRFQPIQMNKACWLTTSDKKQLDEFMRLKTDYIYFDKDHNPVYMAETGWAL
                     NVAIQNHPHITFHTTSEFKTAVE"
     gene            9178..9903
                     /locus_tag="PLL28_09915"
     CDS             9178..9903
                     /locus_tag="PLL28_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNF69681.1"
                     /translation="MRWWHFMFIFLCWQTADAQQPMRLYLLPGQGSDHRIYQNMRFPD
                     NIDTVHLHYLPVEAEETLEHYAARMVSQIDTTLPFSLMGVSLGGMICVEMSDIIHPQQ
                     VFLLSSASSGDEIPDFYHSFRKYPIYHNFNPNLIKYSTFLLQPIYEPDRRTERKTCNS
                     MIREKDADFMKSAVHMIVTWQRTEADNINTPIIQIHGTADHTLPVENIRADYYIDNGS
                     HMMTLTMADKVSAIISAAITVKQ"
     gene            complement(9912..10316)
                     /gene="arfB"
                     /locus_tag="PLL28_09920"
     CDS             complement(9912..10316)
                     /gene="arfB"
                     /locus_tag="PLL28_09920"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HNF69682.1"
                     /translation="MPSADIISECTFKAVRSSGKGGQHVNKTSTKVELYFSIADSALL
                     QDEEKAILLTKLGHLIASDGYLRLTCEEARSQRENKILVIEKFIKLIDKALIPPKKRV
                     KTKKPKAAVEKRLTEKKHRSQIKTGRRNNSEE"
     gene            complement(10316..10765)
                     /locus_tag="PLL28_09925"
     CDS             complement(10316..10765)
                     /locus_tag="PLL28_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNF69683.1"
                     /translation="MRIEEEIKQSKFNNEHHKLLLNLLYTGSWLSIKQIQFFKQFDLS
                     PQQYNILRILRGQHPAPASINLLIERMLDKMSNASRLVDKLHAKGFVDRKPCASDKRQ
                     VDVVISKKGMKLLDQIDKEIMAVDGLLSNLSKTEAKSLNELLDKIRA"
BASE COUNT         3358 a         2366 c         2113 g         3096 t
ORIGIN      
        1 aaacaactga aatgctgatg aattccggaa aggaaagcgc aggtatcttt tcggtggccg
       61 ttattgactc aaaaaatatc gtttgcgccg gcggcgatta ccttgacccg acaaacacag
      121 attacaatta ccttcttgtc tatatcatag aaggcgtttg ggactacccg attccggtaa
      181 gtgaaataaa taaagaaagc tctggttatt attcctgtat tgatgttatt acaaataata
      241 atatcacaac attttttgca tgtggaacag gaggtgtaga cgtttggaga agtactaatt
      301 ttaaaattaa ggtactttcg atgtcaggat ttaatgtttt acagatttcg ccctcgggga
      361 aatatgtatt tctggcagga aacaaagttg taggaaagat ggatatttga ggaataactc
      421 ctacaccata aacaacaaat acaaggcata aaaagaagcc gcaacgccaa gtacgccaat
      481 aatgataaaa ttaacgatgg caaatttctc gcctttatat ttttttggtt cagcctttat
      541 tttcttccaa cctttagacg tcagtatagc acttgctatc agtaataatg caccaattgc
      601 caataaaatc ggagccgcaa acattccggc tgcagcaact cctgctcctg caacggctgc
      661 tcctgtgccc gctaaaacca ttggctccaa aacaggttcc tgctcctctt tctttccggt
      721 atcctccttc acaacagcgt ctactgcatc aatgaaagtg ttatctgcat tgatcaccgt
      781 acccggtgtc gacatatctg tttcaacata aacaacagta ttacttccgg ctgaataatc
      841 agcagattta tctattgaag agggttgatt cccaactaac tcttcagcat ataatccgga
      901 aaacccaatg caacaaattg cgcttaacgc aacgacattt tttaaacgaa tcatttgaaa
      961 cataacggtc aaaaataggg attattatct tgatgccact gccacctgtt gttgaaacaa
     1021 acctagtaca ttggaccact gcagcaggta taagcccgaa ggcagcgatt ccggcaaggt
     1081 gataagcact tctcccccat tggtctcctc gaaaaaaact tccttaccac ccaaatccat
     1141 caatcgaatt atcgtgggca tttcaggttg atttgttgaa atatgtattt catgattagc
     1201 aggattagga tataccatag attctgcctc atccatcatt ctaccaccta ctaacaccgt
     1261 cgccacaaca cttgctgtgc aattatgtgc atcttttgtc atgatagtat acgtgccggc
     1321 acttaatccg gaaaatttct tcgccgtttg ccaggtcaaa ccaccattaa tagaatattg
     1381 tttggagcca tatccacctg ttgactgcac ggtgatcttt ccattgtgtg caccggcaac
     1441 agtttcattc actattgaag taactgtcag ggtaagcaat gcatcctcac tgattaccac
     1501 atttcccatt acaaaggcac cacatgcatc ggtgactttt actttataag ttgcagcaca
     1561 caagtcactt aaaaatccat caaaaggtat ccaggcaccg gcaccgattt tatattgata
     1621 agtatacggt gcacatccat aaataccgtc taattctaaa gaaccatcgc agctgccatg
     1681 acagattgtt ggaatggaat taacaagata tgataaagga gcagcaattt gttcatcagc
     1741 acccatttct attctgcctc ccgtaagtct ggttgccaag tccatatcaa attcatcaac
     1801 aggaatgtaa cttggatttc ctgcattgat acatggcgat gttacttgaa gatgaaaatc
     1861 cgaaatggca ggatttacga ataatggatc agcaagtgaa aaatgtgcat tgtcacccat
     1921 tgttgaaagc agcgcttcat aacctgtaat taaggtaaaa tcaacaaagc ccttcattgc
     1981 catcggtccg cccgaataat aataattgtt gtagtccaga tttacttcgt aggtagaatc
     2041 aatagtttcg gcattgatca tccatggggt agttgatgtg aagaaaatat tattttcaac
     2101 aatgcaattc gttgcatatt gtatccataa ttgaccaaat cctgtattca ggaaatcatt
     2161 gtcatatacc gtatttccat agaaagcaca gttggtaacc attcccgtag ccggataatc
     2221 atatcctcca aaacctagtc cggatttttg attgcgataa ataagattat tccgaactgt
     2281 gatgccacta caaacaaatc ccatattctc acaacctatt tctaggccta tatcactatc
     2341 ggtgatagaa ttattttcaa ctacaatatt tttaccgcca tctacatata cacctgccga
     2401 aaatccgcca ccataaatag aattcgcagc cgttacagca tttcttctgc aaaccccgtt
     2461 tcgcgcctga tttaccgcat cattggtatt tacccaatct tcaccaccaa tcatgacgat
     2521 gccgatatta ttgatatccg tcaatacatt atctgcaatt gtaaatccat caatattacc
     2581 attcactgca attgcctcgc ttggtgcagg atccaggtta gtgagtacat tattggtgat
     2641 gacaatattg gtataggcgg tggtagttcc tgaaccaaca aataatacac ccacggcatt
     2701 atcaccaatc aggtcgtgca tattatttcc ggagatcaaa aaatcatcgc caaatccttc
     2761 gcatagaatg gcatagctct cattgcaatt ggtattgtaa aattcgcaat tggtgatttc
     2821 aatatgactg caattgccat acacattaat gacgggctgg taatttccgt tgtgattggt
     2881 aaaacgcaat ccatctattt tgatgtaatt tttattttcg atgtacatca aagcctgatt
     2941 attgcccaca gtgcttccat ccagaattac cacatcatcg ttataatttt gcaatgtaat
     3001 atattgtcca accacaccgc catcattcca ggtcaattta ccggtgtagg ttcctgccat
     3061 tacgttgatc acatcgccat cattagcctg gttaagtgca aattgaatgg ttttccaggg
     3121 agaggcaatg ccgatgcctg aattgagatt atttcctgcc ggtgaaacat aatagttggc
     3181 gccatctaaa tttattagtg ccatggagat cattatactc agaataatat tcaatcgcat
     3241 ggttctaagg taatgtggac tatcgacata aaaaaacccg gacttttacc tccgggtctc
     3301 ttttcatact actgcacgca ctcaaattcc tgacagataa cgacttaatt catccacact
     3361 gctgctaaac caaaattcct tgttcacgat gaagtaattc tctgtatcta ctgttttata
     3421 atatgcggct ttcgctaatt ccagcctggt gtcttcaaaa ctcatggcat gcatcatttc
     3481 ggcaacctga ttggcagtaa accaattgct attcaaagcc tgtttggcaa tttgcagtct
     3541 tccactctca aaccactggt cttcaataac atccatgagt tgtgtaaagc tgtaaccagc
     3601 catcggcaat ggtttcggcg gcaaaggcgg agtaccgcag gttggataag tattcggcgg
     3661 gtaataagtc tgaggctcgg gaacatatac cggaatgctt ttcaccgact tgatataaaa
     3721 tcgccctgca ttgtccagaa cgcctgttac cagtgaattt gcgtcaatgt agatgtcgcc
     3781 gctatagatc aacttctgcg ctcctgcgtg gccttgttga cctgctgcaa acaaatcaac
     3841 atggtgcaaa ccggctgtca tcgttgccat ggaaaatctc ttaaccggat tggggtaata
     3901 ccgaccatca agctgcacag aaaagtaata actaccctgc gacttgatct ctaaggttga
     3961 attggctacc ggcacccaac cggcaggcgg attggcactt gcaatgctga atggtaccag
     4021 aatggcaatc aatagtttgt gtacgatagt tttcataaaa acctcctttt tcttaacata
     4081 tattccacta tcgtgccaat ttgatacatt tatgataatt ttcttgacaa ctactgatag
     4141 aaagccctaa tacggggcgc ttggcgtatt tttaccccgt gatcaaaaag gaaaccatag
     4201 accgcattaa tgataccgcc cgcatagacg aggtggtagg cgattttgtg cgtttgaaga
     4261 agcgtggtgt caatttgatc ggtctttgcc ctttccataa tgagaaaaca ccttctttca
     4321 acgtatcggt gcccaggggt atctataaat gctttggctg tggtaaaggg ggtaatgctg
     4381 tatcgtttgt tatggaacac gagaaattca cctatcgtga agcattgctc tatctggcga
     4441 acaaatacaa tatcgaggta gaagaaacgg agcgaaccaa tgaagaaatt cttgaagata
     4501 atcgtcgcga aagtctatac atcatcaatg catttgcaca gaattatttt tcagagcaac
     4561 tgctgaattc agaagaagga aataatatcg ggctgagcta ttttgaagaa cgcggactcc
     4621 gcagagaaac gatagaaaca ttcaaactcg gttattgccc ttccgatggc gactcattta
     4681 cgcaatcagc acatcaagcc ggatatcaaa atgaattgtt gcaacatctt ggattgacta
     4741 cagcctatgg aaaagatttt ttccgtggca gggtgatgtt cactattcaa agtgtcagtg
     4801 gtaaaatatt gggatttggc ggaagaacgc ttagtgccga taaaaagcag ccaaaatata
     4861 tcaactctcc ggaaacggat atttacttta aaagtaaaat tgtttacggc atttatcagg
     4921 caagaaaagc cattgctcag cacgatgttt gttttttggt agagggatat atggatgtga
     4981 tatcactgca tcaaggtggt attgaaaatg tggttgcctc atcaggaaca gcacttacac
     5041 aggatcaggt gcgactgatc aagcgctaca cacaaaatct gattatatta tatgatggcg
     5101 atgcagcggg tgtaaaagct gcgctgcgcg gattagatat tgcgctggaa gaaggcatga
     5161 atgtaaaggt cgtgttgctt ccagctgaag atgacccgga tacattggtt caaaaattag
     5221 gtggcgacgg atttaataaa tatgtagagg cacagaaaaa ggacctcata catcttaaaa
     5281 cagatctctt ccttgaagaa gcaaaaggtg atcctgtaaa agttgccggc atcatcaagg
     5341 atattgtagc ctcgatagca aaaattcctg atgccattac gcgcagtctg tatgtaaaac
     5401 aaacggctaa aatgctggag gttgaagagc agatactgat taccgaaact aataaattac
     5461 tgcgcaaaga ggcaactaaa aaagttactc ctgaagacaa agccactgtt gaagctcttg
     5521 attatacacc acctgcaaaa gaacagccac tcttcacttt tcatctcgac actccgcagg
     5581 aacgagatat agtgcgcctg ttacttgaaa acagcaatta tgaaatagaa ggtgaaaatg
     5641 ccatcgccag aatattgcgc aacctcagtg atgttgagat cgatcatccg gaatacaata
     5701 tgatcatcga agaatatcgc aatgcgtttg cacaaaacga atttcttaca caagcccatt
     5761 ttttcaatca ccctgatgag gccataagaa acctgaccat agatttattg caatcgcctt
     5821 acgagttgag cgacaactgg acgaaaatgc atgatgtgcc tattacggac aaacagtttt
     5881 tggtgcgaaa ggacattgtg aaatcaattt cgctgctcaa gctcaaaaag atcattcgta
     5941 tgaagcagga cgttgataat cgcattaaag aattgcaaca cgatacatct gaaaacggca
     6001 ttcaggaaac catgctgtgt caaaaagaga gcattcaatt gcaacagttt atccgcaaac
     6061 tatccactga taccggcact gtcgttttgc ccggcatcct ctgaaatgcc cattgcttct
     6121 ttgcgcattt tctcactcaa ttccgttcca agaaaattat ctatgatatg gtgcacccaa
     6181 tataataccg gtgtaagtac aatagctgca agaattttat aaaagtaatt tacggttgcc
     6241 actgcaaaga aaaatggcaa agtccaatgt tgcggaccta gcacgaaagc aatatacaac
     6301 acgatcaaac tgtcaaatat ctgtgagata gcagttgaac ctgttgcacg caaccaaata
     6361 cgtcgctcac cggtaaaagc tttgatgcga tgaaaaataa aaacatctac aatctgactg
     6421 aataaaaagg cagcaatact ggcgccaatg atccacattc cctgcccgaa aattgcagca
     6481 taagccgcct gcatatccgg aacaccggag tcaacaaaac ttcccggcca aaaggcagca
     6541 ggtttcaaac taattccggc atatacaatt gcgaaagcat atgcaataca aattacagcc
     6601 agataactca atcggcgcac accttttaca ccaaaatatt cattgataat atcagtcatg
     6661 ataaaaacaa aaggccataa tatcacaccg actgtaaaat tcaatgagcc tgcctgccca
     6721 tataaattcc agttaaatgg ctttaacccg agactatctt ccaaagcaaa aaccttcact
     6781 ccgataaatt cagctatgat ggcgttgacc acaaaaaaca cacctagcac aagcaacaag
     6841 atagcaggct tgctagcgaa gggattgtga tgatgtttat ccatatgtta tttctttaaa
     6901 aaaggaaaaa attttgatgt atgcgctgca tatttttcgt attcactaaa agcaagttta
     6961 agttttttct cttcgtaaac tgtcttcctg tcgaaaaata gcaacaagag aatactaatg
     7021 accatgcgcg ttatgctacc ctgaaaaatg gcgaatccta cacaggttaa aaacacacct
     7081 gtatagatcg gatgacgcat acaagcaaat atgcctgttg taatcaggtg cgacccggtt
     7141 ttgggagtcg gattcatggt aagatttctt cgcaattgca aaattggcca ccaaacgagt
     7201 atgaccccaa tcaccccaat caatattccg actaatctac ctgcaatgcc cggttgaaag
     7261 gaatactgcc acggttccca aatgtaaaca gcaaaaaaaa ccaactgaat aaatacaagt
     7321 atcctgtcct ttaccataag ctgccctgaa acaactgaaa catacaactt tttgctgaaa
     7381 catcgtattt tatggtgtga gtaagccctt attggtatct ttgcagccgc ttaagagggc
     7441 agtatcatgc gattagagac ggaaattaac aaaagaagaa cctttgccat tatcagtcat
     7501 ccggatgccg gaaaaactac acttacggaa aaatttctgt tatacggtgg agctattcag
     7561 gttgcaggtg cagtaaaaag caataagatt aagaaatctg ctacgagcga cttcatggaa
     7621 attgaacgtc aacgtggtat ctccgtggct acctcggtga tgagttttga ataccaaggt
     7681 atcaacgtca atctcctgga tactcccggt cacaaagact ttgctgaaga cacttatcgc
     7741 acactcacgg ctgtcgacag tgttatcctg gtaatcgatt gcgtaaaagg tgttgaagag
     7801 caaactgaac gattgatgga ggtttgccgt atgcgcaaaa cgccggttat cattttcatc
     7861 aacaaaatgg accgcgacgg aaaagatcct tttgatctat tagatgaatt ggaagataaa
     7921 ctaagtatta aagttcgtcc tctctcctgg cctattaaca ccggaacaca ttttaaaggc
     7981 gtttattcta tttatcacca caaaataaat cttttccgtc cgggttctca acgcgtagaa
     8041 gatgatgtaa tcaccattga taatctcaac gacaaaacac ttgacacgca agttggcgaa
     8101 cgcgatgcag aacaattacg tcacgatatc gaattgattg aaggggtgta tgatgctttt
     8161 gatacaaatc gatatttaaa agcagatgtt gcgccggtat tttttggttc tgccatcaat
     8221 aattttggtg tacaggaatt attggaaaca tttattcaaa tcgcaccggt tccgcaaggc
     8281 agagataccg ataaacgtta tgttgatgcc gatgaagaaa aattcaccgg ttttattttc
     8341 aagatacatg cgaatataga ccctcgacat cgagacagaa tcgcattttt acgcgtgtgc
     8401 agcggtcact tcgaacgcaa tagtttctat catcacgtgc gcctcgataa aaatttgcgc
     8461 ttcaacaatc catatgcatt tcttgcagcg agcaaggatg tggtggatga ggcctggccc
     8521 ggcgatgtga ttggtttata tgatacaggc aatttcaaaa tcggcgatac acttaccgaa
     8581 ggtgaagacg tgatgttccg tggcatccca agtttctctc ccgaaatttt taaggagctg
     8641 gtgaatatgg atccgatgaa aacaaaacag ttggaaaaag gaatcgagca attaaccgat
     8701 gaaggtgttg ctcagctttt cgtacataat aacggattca aaaaagtagt tggcactgtc
     8761 ggcgaactgc aatttgaagt aattcaatat cgcctcgaac acgaatatgg tgcaaaatgt
     8821 cgttttcaac ccattcaaat gaataaggct tgttggctta ccacatctga caagaaacaa
     8881 ctggatgaat ttatgcgctt gaaaacggat tatatttatt ttgacaaaga ccataatccg
     8941 gtatacatgg ccgaaacagg ctgggcatta aacgtggcca ttcaaaatca tccgcatatc
     9001 acttttcata caacctcgga atttaaaacc gcagttgaat agcactttga tatctgattg
     9061 tgttaccggg ttgatcatcc gatattaagc cattgtttca agtttgattt cctgatcgaa
     9121 tacaacaagc attgttttcg gattgcaatt ttcataaaac tcctgtaatt tcgcttaatg
     9181 cgatggtggc atttcatgtt catttttctt tgttggcaaa cagctgacgc acagcaaccc
     9241 atgcgcttat atctcctgcc cggacaaggt tccgatcatc gcatttatca aaatatgcga
     9301 ttccctgata atatagatac cgttcatttg cactatctcc ctgtagaagc tgaagaaaca
     9361 cttgaacatt atgctgcgcg aatggtctcg cagatcgata ctaccctgcc cttttcatta
     9421 atgggtgttt ctcttggtgg aatgatctgc gttgaaatga gcgacattat acatccacaa
     9481 caagtgtttt tgttgtcaag tgcatcttca ggagatgaga tcccggattt ttaccacagc
     9541 tttagaaaat acccgattta ccataatttc aatccgaatc tgatcaaata ttcaactttt
     9601 ctgctgcaac ctatctatga acccgaccgc cgcacagagc ggaaaacatg caatagtatg
     9661 atcagagaaa aggatgccga ttttatgaaa agtgctgtgc atatgattgt tacctggcaa
     9721 cgcacagagg ccgacaatat taataccccc atcattcaga tccacggcac tgccgaccac
     9781 acattacctg ttgagaacat acgcgcagat tattacatcg acaatggatc gcacatgatg
     9841 acactcacaa tggcagataa agtgagcgct attatttctg ctgcaattac tgtaaagcaa
     9901 taatcacttc actattcttc tgaattattg cgtcgcccag tttttatttg ggagcgatgt
     9961 ttcttttcgg tgagacgctt ttctactgcg gctttgggct tttttgtctt tacgcgtttt
    10021 ttaggcggta tcaaggcttt atctatcagc ttgataaact tttcaattac caatatttta
    10081 ttttcacgtt gcgatctcgc ctcctcacaa gtcaagcgca aataaccatc agatgcaatc
    10141 aggtgaccta acttagtcag taatatcgct ttttcttcat cttgcaataa cgccgaatcc
    10201 gctatggaaa aatacaactc tactttcgta gatgttttat tgacgtgctg tccgccttta
    10261 ccactgctgc gaaccgcctt aaaagtacat tccgatatga tatccgcaga tggcatcagg
    10321 ccctgatctt atcgagcaat tcgtttaagg attttgcttc agttttcgac aggttagaca
    10381 acaacccatc aacggccatg atctccttat caatctgatc gagcaatttc atccctttct
    10441 tagagataac aacatctacc tgacgcttat cactggcaca gggtttacgg tcaacaaagc
    10501 ctttcgcgtg caacttgtca actaacctac tggcattact catcttatct aacatcctct
    10561 ctatcaataa gttaatactt gccggagcgg ggtgttgacc tctgagtatc ctaaggatat
    10621 tgtattgttg cggcgaaagg tcaaattgct tgaagaactg tatctgcttg atacttagcc
    10681 aacttccggt atacaataag ttcaacagga gtttatgatg ttcattgttg aacttcgact
    10741 gcttgatctc ctcttcaatg cgcatgtgtg atggtttgtg gtttaggttg taagcaaaga
    10801 taacaatcca tggcatttta acaagagact tttctcttga aacacaagcc ggataagggt
    10861 ctgatattag gaacatatgc atttaatttg aatgcgtttt tcaataatcc gaatttcaat
    10921 accacaattc ttt
//
LOCUS       DAOQHY010000113        10927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_2996_length_10927_cov_5.576619, whole genome shotgun sequence.
ACCESSION   DAOQHY010000113 DAOQHY010000000
VERSION     DAOQHY010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10927
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2996_length_10927_cov_5.576619"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            554..2188
                     /locus_tag="PLL28_09930"
     CDS             554..2188
                     /locus_tag="PLL28_09930"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69684.1"
                     /translation="MLFRFTFITVLLCWFLTVTNAQVYPVPEITWEKCFGGSGNDSYF
                     DTEKTPDGGYISVGESTSTDYDLAGVCCPGVTKSLWLCKLDSNYNIQWQKLLQAIPTD
                     VRIDCRIFANKDQTYTLFETIDGDGVVPLCTCNHSASQDIWMFTVDNFGNIISQKCFG
                     GSAYDLMNNVSRCEDRGYIIGASSGSGNGDVGIHYGSPFTTDAFIVKTDSLGNIQWTK
                     TLGGTGWEGAFVSGISDNRCLINIGASSNDYDLTGKMPLGETMVNWLMITDSAGNILQ
                     DTVVRAYDGKLDLNFPYEYKKNNYLIVGYISMDTGMYAMAYGGGDALIAVFDSSLALE
                     KVVQFGGTKLDNIYKMNKLNDSTIYFTGRTYSSDYDAQECRGLWDAFIVKTDTSLNKL
                     WSRSIGGSSADVFSNLFQSDSTIVLFGSSAINSADDGDILNAHYDEEIGLSSDSWVIF
                     LNENVPYDTVYLIPEIKIYPNPSSNQVNIVISADVDMDYNLAIYSLDGKMALSESLSS
                     NIVNEVKVNSLPSGYYIFSIRDGSKNEIVSKKILIL"
     gene            complement(2343..3950)
                     /locus_tag="PLL28_09935"
     CDS             complement(2343..3950)
                     /locus_tag="PLL28_09935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNF69685.1"
                     /translation="MKKLITLLLMTALVSIDQRLTAAPLLCSADFTVTISGATASFNG
                     SGTGITPFYSWDFGDGSYGSGEDISHTYTFNGTYIVCLTVWGADSCMAMTCDSVVITG
                     AAAGGSCHADFTYFIAGTDVSFDNNSDAGGAIDADFFWSFGDGGTSTAVNPEHSFAPG
                     TYEVCLDIFTSDSCSDTYCTVIAVGGIIDSLDSLECNAYFTYDFLMGEVNFTNLSDDG
                     GAAFSSYFWDFGDGTTSTKENPSTTFDPGWHTVCLTINTSDSCSSTYCTDIYIWDGGG
                     DTTVCEAGFDFTVTGMSADFTNTSSGGGAFITSYNWTFGDGGTSTATNPEHAYAAEGI
                     YNVCLTIYTTDSCSSTYCETVVISTGDTTGCTALFTYDFGITPWGIFTTNLSDDGGTG
                     DASYFWDFGDGTSSTEEEPAHNYAGGGDYLICLTISTDSCEDTYCSSVSIAPTPIAET
                     ELFEGLTLYPNPARDVINIHVALHADVTCILTDLSGRELSVRTLQANAGNTEHQINIA
                     DLESGIYTILLIDAAGNRAMEKIMVVR"
     gene            complement(4071..5282)
                     /gene="porV"
                     /locus_tag="PLL28_09940"
     CDS             complement(4071..5282)
                     /gene="porV"
                     /locus_tag="PLL28_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HNF69686.1"
                     /translation="MQSTLKITTIAVVLTMISTTTRAQISLGNGTGDDYLNTVTTAVP
                     FLRIAPDARSGGMADVGLAISPDANSIFWNPAKTVFIDDEHPYGMSLTYTPWLSNLVN
                     DIYLAYLNGYWKVDELSAVSASLRYFSLGTITFTDASGNVLQDFRPNEFAFDVAYSRK
                     LADNLSAGLDLKYLYSNLATDQVVNGVQIKPARGVAADISMFYHTKFDTGDKDSELGV
                     GLNISNIGNKITYTSSIEKDFIPTNLGLGAAYTVNFDSYNALTFAVDMNKLLVPTPDS
                     TDADNDGILDFKEESTVSGMLGSFSDAPNGASEEFHEIMWSTGLEYWYAERFAVRTGY
                     FYEHATKGGRQYFTVGLGLRYSVFGLDFSYLVPTSNNQNPLDNTLRFTLIFNLENLKG
                     GGSTEDSGSDS"
     gene            complement(5343..9245)
                     /gene="porU"
                     /locus_tag="PLL28_09945"
     CDS             complement(5343..9245)
                     /gene="porU"
                     /locus_tag="PLL28_09945"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HNF69687.1"
                     /translation="MKLTTSRYRLTAAMLTCLIVNAVSAVAQISVDRTLNWSATPRSF
                     SGEGVNEVFSLQFTGASYTTEGEVILPLYAESFPASGNKIVSAKINNAQYAPIETANA
                     KIAAGMRTKIRNEIKINAGIGYARKKPEANISFIPLRINPSTGNIEKLVSFTLDIVTA
                     IDQQLKITGEDDFTGNSVLSSGSWYKFAIKREGIFKVDRAVLSSMGISGTVSFSTFGV
                     FGNGGGMLPESNLTARYDDLQENAIYKYDANGNGMFEDGDYILFYGQAPHRWYYNTVT
                     NTFKHKNNIYSDQTCYFITTDRGNAKYITAQASVAGENYNTSSYDIMSFIDQDKVNLL
                     NSGRAWYGDIIDIYTPNRTYNFTLPDLITSEYILFRASAAGNSTSGSITIDLKANGAS
                     LPSDITISEVSEGFSETYAYENGVETGFNSTSSSIAISMDYYGSSVATAWVNYIELIG
                     RANIVYNNTPLFFRDARTIGDGRITKFTIDAPNGIQIWDITDRINAKLQNYTVSGSDL
                     TFTLATDTLKEFVAVDPNDAYGADDITFIEKVVAQDLHNDANQPDLVIVSHPSFMSEA
                     RRLAGWHHDVDGLDTLVVDVNKVYNEFSSGVQDISAIRDMMRMFYERAGGDESLMPQY
                     LLLFGDASFDYRFLAFDEEKNTLKIPCFESNESLARAVSYGTDDYYGFLDPTEGDNIE
                     DINEKLDVGIGRLPVMTVDEATIMVDKIMHYKSPESLGSWRNALTFLADDEDYNTHVE
                     DAEELAEYMTTNYPVYNVDKIFFDSYQQIPGAGGERYPEVETAIDNKIFTGSFIMNYL
                     GHGNEQNWAQERVLSVDDINSWDNYDKLPLFITATCSFSRYDNPDLRSAGELVLLNPQ
                     GGGIALVTTVRLVIASANYDLNSNLIEHLFEPVDGEYPRLGDAVRLGKNGVTASAQNN
                     RKFILLGDPALQLNYPHQRVVTKTVNGFDVAVYTDTLKALEKVTITGEVTDESGVKLT
                     GFNGVVYPAIYDKPILINNLVNDPTFSGVGGYGPSEPFSFYLQKNALYKGKASVVNGE
                     FTFTFIVPKDISYTYGNGKLSYYADNGTDDANGYNTNVIIGGSSDDAAADNVGPDVDV
                     FMNDESFVFGGLTDENPVLLIKLIDSSGINALGNAIGHDITATLDDDQQTMLKLNEYY
                     EADLDKYQSGSVLYPLNDIAEGRHTVTVKAWDVYNNSGDGYTEFVVAETADLALDHVL
                     NYPNPFTDNTSFWFEHNRPGDVLDVKIEIFTVSGKRVKTIQQQVITDGYRVDDITWDG
                     LDDYGDVIGKGVYVYKLSVKAASDNSKASEFQKLVILK"
     gene            9271..10341
                     /locus_tag="PLL28_09950"
     CDS             9271..10341
                     /locus_tag="PLL28_09950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HNF69688.1"
                     /translation="MKVFLERKYQKNIATSRSFKGQYSLLCITGLLLISFLSAQKLSA
                     QDLRYSQWHAAQTTVNPAFAGAFGRPRIILNFRDQWPMMPQSYVSYRAAMDAYVPKIR
                     SGLGVTIDQDNQGDNLLQSTRFGMQYMYQARLGAEWALNFGLSLDYRQYRINWQDIQF
                     YDQISLLTGFNDVSGNPNPTAEPAPGKNQVGFVDLGSGLLLYNKSAYLGFSFNHITNP
                     VVSFYGTDNSELPGSLNAQAGVFIGGKREKDLLVNPYALWSHQAGFNQIQTGVYVKKS
                     LLLTGLAVKHNTVGLSDVVLLAGVSQETIRFAYSYDVSTGPLAGLTGGAHEVSLVITL
                     KENAGRAFRNSQKSMLDCPSEL"
     gene            10395..>10927
                     /locus_tag="PLL28_09955"
     CDS             10395..>10927
                     /locus_tag="PLL28_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNF69689.1"
                     /translation="MKRNLHPVLVLSMTVLVLAFTTSCKKRSAVSGFKYNDSKWGGFE
                     AHRFDGQATGPNLVLVQGGTVVQGTTEQDVTFEYNNIPRRVTVSSFYMDETEISNLHY
                     REYVYWVTKQFPDFPQVIKNSLPDTLVWREELAFNEPYVQYYFRHPSYQNYPVVGVSW
                     LQATEFCKWRSDRVNEM"
BASE COUNT         3129 a         2276 c         2343 g         3179 t
ORIGIN      
        1 cccacagatt gcgcagagtt tgatgcatcc attcctaaaa ctccgccaac tccgcaaact
       61 ccgtgattta tacaggtgtg tgtgtacctc gccataggtg ggtcgtgaat cgtgcaggtg
      121 ccgagggcag accgttttgt aataaaaatc aagcctgccc cgaagtgtaa atttttgtag
      181 cgcagagaag aaattttcta cttccggggc taatgaagaa tctccatttc gagaagaaca
      241 taaacccaac cctgaattag aaaaatgctc cgctccgcta cgcattttgt cacttcaggg
      301 caggccctga gggaggggat agggaggtct gccgaaggca gatctttgtt gttatggata
      361 taaatttcaa gccaatcctg ttgaaactta ttgttgcagg ttaggaggat tgggtaggtt
      421 taaagttcta aacctgtgtt tgaaagatac ggttcgatgt aggtttagta aatttagggt
      481 tgttttattg taaccactga ttagaagatt gctgcaaaat gccatttgtt taaaaataca
      541 tatccaatat taaatgctat ttcgctttac atttattact gttttactat gttggttcct
      601 tacggttacg aacgctcagg tttatcctgt tcctgaaatt acatgggaaa agtgttttgg
      661 aggttcaggg aatgacagtt attttgacac tgaaaaaaca cctgatggag gctacatctc
      721 agtaggtgaa agcacttcca ctgattatga tcttgcaggt gtttgttgcc cgggtgtaac
      781 aaaaagttta tggctctgta aattagacag taattacaac atccaatggc aaaaactact
      841 tcaggcaatt cctacagatg tcaggatcga ttgccgtata tttgcgaata aagaccaaac
      901 ttacacactt tttgaaacaa tagacggtga tggtgtggtt ccgctctgca cctgtaatca
      961 cagtgcgtcg caggatatat ggatgtttac tgttgacaat tttggaaata ttatcagtca
     1021 aaaatgtttt ggtggtagtg cctatgatct gatgaataat gtatcaagat gtgaagatcg
     1081 tggttacata attggtgcct catcaggttc aggtaatggt gatgtaggca tacattatgg
     1141 gtcaccattt accactgatg cctttattgt caaaacagat tctcttggta atatacaatg
     1201 gacaaaaacc ttaggaggaa ctgggtggga aggtgccttt gtgtcaggta tttcggataa
     1261 cagatgccta ataaatatag gagccagttc aaatgattat gacctgacgg gcaaaatgcc
     1321 tttaggtgaa actatggtga attggttaat gattacagat tctgctggaa atatattgca
     1381 agacactgta gtaagggcct acgacgggaa actcgacctt aattttccct acgaatataa
     1441 aaaaaataac tatttaattg ttggttatat ttcaatggat acaggcatgt atgcaatggc
     1501 atatggtggt ggagatgcat tgatagctgt ttttgattca tctcttgctc ttgaaaaagt
     1561 cgttcagttt ggcggaacga aattggacaa catttacaaa atgaataaat taaacgactc
     1621 gacaatatat tttacaggca gaacttattc atcggattat gatgcacagg agtgtcgtgg
     1681 cttatgggat gcatttattg taaaaacaga cacctcgctt aataaattgt ggtctcggag
     1741 tattggaggt agtagcgctg atgttttttc taatctattt caaagtgatt caactatagt
     1801 tctttttggc agtagtgcaa ttaatagtgc agatgatggt gatattttga atgcgcatta
     1861 tgacgaagaa atcggccttt caagcgattc ctgggtcata tttttaaacg aaaatgtacc
     1921 atatgacacg gtatacttga tccctgaaat taaaatttat cccaacccat caagtaatca
     1981 agtaaatatc gtgatcagcg ctgatgttga catggattat aatttggcta tttattcttt
     2041 agatggaaaa atggcactaa gtgaaagttt gtcttcaaat attgtaaacg aagtcaaggt
     2101 aaattcgctt ccttccgggt attacatttt ctcaattcga gacggatcga aaaatgagat
     2161 cgtatcaaaa aaaatattga ttttatagtt tattggttat agaagcggag acaattttct
     2221 acttccgggg ctaatgaaga attctaattt tatgatgatc aagatatttc atcgatattg
     2281 aaatcaaaaa caaaaaaccc gaaacgcaat cgcttcgggt tttcttgtca gcagaattaa
     2341 actcaacgca ctaccattat tttttccatg gccctgttac cggctgcatc gatcaacagg
     2401 atggtgtata taccgctttc aagatcggcg atattgatct gatgttctgt atttcctgca
     2461 ttcgcttgta aggtgcggac acttaattcg cgcccactca agtctgtgag aatacaagtc
     2521 acatcggcat gcaatgctac gtggatattg atcacatctc ttgccggatt tggatacaag
     2581 gtaagtcctt caaataattc tgtttctgct atcggcgttg gagcaatgct tacactgctg
     2641 caataggtgt cttcacaact atcggtagaa attgtaaggc aaatcaggta atcgccaccg
     2701 cctgcataat tgtgtgccgg ctcttcttct gttgagcttg tgccatcacc aaaatcccag
     2761 aaataacttg catcgcctgt accgccatca tctgaaagat tagtggtgaa aataccccaa
     2821 ggagtgattc cgaaatcgta agtgaataag gctgtgcatc ctgtggtatc gccggtagat
     2881 attactacag tttcacaata ggtgctggag catgaatcag ttgtataaat ggtcagacac
     2941 acattgtaaa tgccttcagc agcatatgca tgttcagggt tggtggcagt ggatgtaccg
     3001 ccatcaccaa aggtccaatt gtatgatgtg ataaatgccc cgccacctga tgaagtattc
     3061 gtgaaatcag cactcatacc tgtcaccgtg aaatcgaaac cggcttcgca aacagtggtg
     3121 tcgccaccgc cgtcccagat atagatatct gtgcaataag tactgctgca gctgtcgctt
     3181 gtattaatgg tcaagcatac agtatgccat cccggatcga atgtggtgct tggattttct
     3241 ttggtagatg tagtgccatc accaaaatcc cagaagtaag aggagaatgc tgcaccgccg
     3301 tcatctgata ggttagtaaa attcacttca cccattaaaa aatcgtacgt gaaataggca
     3361 ttgcactcga gtgaatctaa tgaatcaata ataccaccta cagctatcac ggtgcaataa
     3421 gtatcactac aactatctga agtgaaaata tcgagacata cttcataagt gcccggagca
     3481 aaactgtgtt cgggatttac tgcggtagaa gttccgccat cgccaaagct ccaaaagaaa
     3541 tcggcatcga tagcaccacc ggcatctgaa ttattatcga agctcacatc tgttccggca
     3601 ataaagtagg tgaagtcggc atggcaactt ccgccagctg cagcacctgt gatcacgaca
     3661 ctatcgcatg tcatggccat gcaactgtcg gcaccccaaa cggtcaggca gacgatatag
     3721 gtgccattaa atgtgtaggt atggctaatg tcttcgcctg aaccatagct gccatcgcca
     3781 aagtcccagc tataaaatgg ggtgatgccc gttcctgagc cattaaatga tgcggtagca
     3841 ccggaaatag ttaccgtaaa atcggcagag catagcagag gagcagctgt gagccgttgg
     3901 tctattgaaa ccagtgcagt catgaggagg agtgtgatca attttttcat atcattcaaa
     3961 ttttattgta acaacatcct acacgacact caggataaaa tggttgggtt agacaccaaa
     4021 aaaaaagagg cgaacatgtc gcctctttaa taatatgttg cgggtcgtta ttaactgtcg
     4081 gaacctgaat cttcagtgct accgccacct ttcaagtttt ctaaattgaa aatgagggtg
     4141 aaacgaagtg tattatccaa tggattttgg ttgttgctgg taggcaccag atagctgaaa
     4201 tcaagtccga atacgctata acgcaatcca agacctacag taaagtattg gcgaccacct
     4261 tttgtggcat gctcatagaa atagccggtg cgcacagcaa aacgctctgc ataccagtat
     4321 tcaagacctg tgctccacat gatctcatgg aattcttcgc tggcgccgtt tggtgcatca
     4381 gagaatgaac caagcatacc gctcacggtg ctttcttctt tgaaatcgag gataccgtca
     4441 ttgtcggcat ctgtagaatc cggtgtaggt accaataatt tgttcatatc aacagcaaat
     4501 gtcaacgcat tgtagctgtc gaaattcacg gtataagccg ctcccagtcc aaggttggtt
     4561 gggataaagt ctttttcgat agagctggta taggtaatct tatttccgat attggagata
     4621 ttcaatccca cacccaattc agagtcttta tctcctgtat cgaatttggt atggtagaac
     4681 atgctgatat cagcagccac accgcgagca ggtttaattt gcacaccatt caccacctgg
     4741 tcggtagcga ggtttgaata aaggtatttc aagtcaagac ctgcagataa attatcggca
     4801 agcttgcgtg agtaggctac gtcgaaggca aattcgtttg gacggaaatc ctgcaataca
     4861 ttgccggaag catcggtaaa tgtgatggtt cccaaagaaa aatagcgcaa tgaggcactt
     4921 acagccgata attcatccac tttccaataa ccgttgaggt acgcaaggta aatgtcattt
     4981 acgaggttgc ttaaccatgg tgtataggta agcgacatac cgtaagggtg ctcatcgtcg
     5041 ataaaaacgg ttttcgcagg gttccagaag atagagtttg catcagggga aattgccaaa
     5101 ccaacgtcag ccatcccgcc ggaacgcgca tcaggagcga ttctcaggaa gggtactgcc
     5161 gttgttacgg tattcaaata gtcgtcgccg gtgccgtttc ccagggatat ctgagcacga
     5221 gtggtggtgc tgatcatcgt caaaaccacg gcgattgttg taatctttaa tgttgactgc
     5281 atattatgtt tgataaaatc tagggttcgc aaaaatacaa tattttgtaa cgtatcgggg
     5341 cactacttta gtataacaag cttttgaaat tctgaagcct tagaattgtc gctggcggct
     5401 ttaacactca gtttgtagac ataaaccccc tttccgatca catctccgta gtcatccagc
     5461 ccgtcccaag tgatatcatc taccctgtat ccgtcggtta ttacctgctg ctgtatggtt
     5521 tttacgcgct taccactcac agtgaaaatc tcgattttaa catccagaac atctccgggt
     5581 ctattgtgtt caaaccaaaa actggtattg tcggtgaaag gattcggata gttcaaaaca
     5641 tggtcaagcg caaggtcggc agtttcggct accacaaatt ctgtataacc atcgccactg
     5701 ttattgtaaa cgtcccaggc ttttacggta accgtgtgtc ggccttcggc gatgtcgtta
     5761 agcggataga gtacagaacc actctggtat ttgtcaagat cagcctcata atattcgttg
     5821 agtttgagca tggtctgctg atcatcatca agtgtagctg taatatcatg accaatggca
     5881 tttccaagcg cattaatacc gctgctgtcg atgagcttga ttaacaaaac cggattttca
     5941 tctgtcaatc cgccaaaaac aaatgattca tcattcatga acacatctac atcaggaccg
     6001 acattatctg ccgcagcatc atctgatgaa cctccaataa tcacatttgt attatatccg
     6061 ttggcatcgt cggtgccatt gtcggcataa taactgagct tgccatttcc gtaagtataa
     6121 gaaatatcct taggcacaat aaatgtaaag gtgaactcgc cattcacaac acttgcttta
     6181 cccttataca aggcattttt ttgcaaatag aaactaaatg gctcgctagg cccgtatcca
     6241 ccaacgccgc tgaatgttgg atcattaaca agattattta tcaggatggg cttatcataa
     6301 attgccggat aaacaacgcc attaaaaccc gtcaatttaa ctccgctttc atctgtcact
     6361 tctccggtga tggttacttt ttcgagtgct ttcaaagtat ctgtgtatac ggcaacatca
     6421 aatccattca cggttttcgt taccacgcgt tgatgcggat aatttaattg taatgcagga
     6481 tcaccaagca aaatgaattt gcgattgttt tgcgcggaag cagttacacc atttttccct
     6541 aatcgcacag cgtcacccaa acgcggatat tccccgtcga caggttcaaa caagtgttca
     6601 attaaattac tgttgaggtc ataatttgct gaggcaataa ccagtcgtac ggtagtaacc
     6661 aatgcaatgc cgccaccttg cggatttaat aacaccaatt cacctgcaga gcgtaaatca
     6721 ggattgtcgt atcgcgaaaa tgagcatgtt gcagtaataa ataatggcag cttgtcgtaa
     6781 ttatcccaac tgttgatgtc gtcaacagac agcacgcgct cctgcgccca gttttgttca
     6841 ttaccatgac cgagataatt cataataaat gaacctgtga aaattttatt gtcaatagct
     6901 gtttctactt ccggataacg ctcaccaccc gctccgggaa tttgctgata cgaatcgaaa
     6961 aatattttgt cgacattata caccggataa tttgtggtca tgtattccgc caattcttct
     7021 gcatcttcaa catgggtgtt gtagtcttca tcatctgcca aaaatgtcaa ggcatttctc
     7081 cagcttccta aagattccgg cgatttataa tgcatgattt tatcgaccat tattgtggct
     7141 tcatcaactg tcattacagg taatctgcca atgcctacat ccagtttttc attaatgtct
     7201 tctatattgt cgccttctgt aggatctaaa aatccatagt agtcgtcagt cccgtaagat
     7261 actgctctgg caagcgattc attgctttcg aagcatggaa ttttgagtgt gttcttttct
     7321 tcatcgaagg caagaaaacg ataatcgaaa gaggcatcgc caaacaataa taaatattga
     7381 ggcatcagtg attcatctcc tcctgcacgt tcataaaaca tgcgcatcat gtcgcggatg
     7441 gcagaaatat cctgcacgcc ggaactaaat tcattgtata ctttatttac atcaaccacc
     7501 aatgtatcca atccgtcaac atcgtgatgc catcctgcca atcgacgtgc ttcactcata
     7561 aaagaagggt gacttacaat tacaagatcg ggttgattag catcattgtg caggtcctgt
     7621 gcaacaactt tttctatgaa ggtaatgtca tcggctccat atgcatcatt cggatcaaca
     7681 gcaacaaatt cttttaacgt gtcggttgcc agtgtgaagg tgaggtcact tccagaaaca
     7741 gtataattct gtaatttggc attgatgcga tcggtgatat cccaaatttg aataccattc
     7801 ggtgcatcga tagtgaattt tgtgattctg ccatcgccga tagtgcgcgc atctctgaaa
     7861 aataatggag tgttgttgta aacaatattg gcgcgaccaa ttaattcgat ataattcacc
     7921 caggcagtag ctacagaaga accataatag tccatgctga tggcaattga agaagatgtt
     7981 gaattaaagc ctgtttcaac accattttca taagcatagg tttcgctaaa tccttctgaa
     8041 acttcagata tggtaatgtc tgatggaagg cttgcgccat tggctttcag gtcgatggtg
     8101 atggagcctg aagtgctgtt tcctgcagcg cttgcgcgga ataaaatgta ttctgatgta
     8161 attaaatcag gcagggtgaa attatacgtg cgattaggtg tatagatgtc aatgatatcg
     8221 ccataccacg cgcgaccgga gttgagtaaa ttcactttat cctgatcgat aaaactcatg
     8281 atatcatagg atgaagtatt gtagttttca cctgcaacag aagcttgtgc agtaatatat
     8341 tttgcattgc cgcgatcagt ggtgataaaa taacaggtct gatcggaata aatgttgttt
     8401 ttatgcttga aggtgtttgt tacggtatta taataccatc gatgtggtgc ctgaccataa
     8461 aataaaatat aatctccatc ctcaaacatt ccattgccat tggcatcata tttataaatg
     8521 gcattttcct gcaaatcgtc atagcgtgca gtgagattgc tttccggtaa catgccgcct
     8581 ccgttgccaa aaacaccgaa ggtagaaaaa gacacagttc cacttatgcc catactactt
     8641 aatacagcgc gatcaacctt gaaaataccc tcgcgtttta tagcgaattt ataccaactg
     8701 ccacttgata ataccgagtt tccggtgaaa tcatcttctc ccgtaatttt taattgttga
     8761 tcaattgctg ttacaatatc aagcgtaaat gaaacgagtt tttcaatatt acccgttgaa
     8821 ggatttatgc gcaaggggat aaaactgata ttcgcttcag gcttttttct tgcataaccg
     8881 attcctgcat ttattttaat ttcatttctg atttttgtcc gcatgcctgc agcaattttg
     8941 gcattcgctg tttcgatagg agcatactgt gcattattta ttttggcaga aacaatttta
     9001 tttccggagg caggaaatga ttcggcatat agcggtagaa ttacctcgcc ttcggtagtg
     9061 tatgaggcac cggtaaattg caaactgaat acttcattta ctccctcccc ggaaaatgat
     9121 ctgggcgttg ctgaccagtt gagtgtgcga tccactgaaa tttgtgcaac agcagatact
     9181 gcattcacaa tcaaacacgt taacattgca gccgttagcc tgtagcgtga tgtcgtcaac
     9241 ttcatgaact tcaaaatcag gtggttttag gtgaaagtgt ttcttgaacg caaatatcaa
     9301 aagaatattg ccacttcccg gtcatttaag ggtcaatact ccctattgtg tataacaggt
     9361 cttttgctga taagtttcct cagcgctcaa aaactatctg ctcaagatct gaggtacagt
     9421 cagtggcatg ccgcccagac cacagtaaat ccggcatttg caggagcctt tggccgcccg
     9481 cgtatcatcc tcaatttccg tgatcaatgg ccgatgatgc cccaatctta tgtttcatat
     9541 cgcgccgcca tggacgctta cgtgcctaaa atccgaagcg gactgggtgt taccatagat
     9601 caggataacc aaggcgacaa tctgttgcaa tctacccgct tcggcatgca atatatgtat
     9661 caggcgcgat tgggagctga atgggcattg aattttggtc tcagtttaga ctacaggcag
     9721 tacagaataa actggcagga tattcaattc tatgatcaaa tcagtttact caccggtttt
     9781 aatgatgtgt caggcaaccc aaacccaaca gcagagcctg ctccgggtaa aaaccaggtc
     9841 ggatttgttg atctgggatc gggattgttg ctttacaata aaagtgctta ccttggattt
     9901 tcattcaatc atatcaccaa tccggtagtg tcgttttatg gcaccgacaa cagcgaacta
     9961 cccggctctt tgaatgcgca ggcaggggta tttattggtg gaaaacgtga aaaagacctg
    10021 cttgtaaatc cctatgcttt atggtcacat caggcaggtt ttaatcaaat acaaacaggt
    10081 gtttatgtaa aaaagagtct tttgttgacc ggtttggcag taaaacacaa tactgttggg
    10141 cttagtgacg ttgttttgct tgccggtgtt agtcaggaaa cgatacgttt tgcctacagt
    10201 tacgacgtca gcacggggcc tttggcaggg cttaccggag gggcgcatga ggtatcgctg
    10261 gtcattaccc tgaaagaaaa cgccggaagg gctttcagaa acagtcaaaa aagtatgctg
    10321 gactgccctt cggaattgta aatctcaaaa taagtttcct acatttgcgt taactctcaa
    10381 gtaaaaaaga aagtatgaaa agaaacctgc atcctgtatt ggttctgtcc atgacagtcc
    10441 tggtgttggc ttttactacc tcatgtaaaa agaggtcggc agtttccggc ttcaaataca
    10501 atgattccaa atggggtggt ttcgaagcac atcgctttga cggtcaggct acaggcccca
    10561 acctggtgct cgttcagggc ggtactgttg ttcaaggtac cactgaacaa gatgttacct
    10621 tcgaatacaa taacatccct cgccgtgtga ctgtttcttc attctacatg gatgaaacag
    10681 agatttcgaa tctccactat cgcgagtatg tgtattgggt aaccaagcag ttccctgact
    10741 tccctcaggt aatcaaaaac tcattgccgg atactttggt atggcgtgag gaacttgctt
    10801 tcaacgagcc gtatgtgcaa tactatttcc gccacccatc ttaccaaaac tatcctgtag
    10861 ttggggtatc atggttacaa gctactgagt tctgtaaatg gcgcagcgac agggtgaatg
    10921 aaatgat
//
LOCUS       DAOQHY010000114        10710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_3080_length_10710_cov_6.315345, whole genome shotgun sequence.
ACCESSION   DAOQHY010000114 DAOQHY010000000
VERSION     DAOQHY010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10710
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3080_length_10710_cov_6.315345"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PLL28_09960"
     CDS             complement(<1..556)
                     /locus_tag="PLL28_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNF69690.1"
                     /translation="MTEAYIVAGLRSPVGKAPRGMFRFMRPDDIAVQVITQLLAQIPQ
                     LEKSRIEDCIVGNAVPEAEQGLQIGRQIAMRALDKSTAGITINRYCASGVEAIAIATA
                     KIRSGMADCIIAGGTESMSLVPTAGWKTSLNYEVAAEHPEYLIGMGLTAENVAKEFNI
                     SREDADAYAFHSHRKATEAIDKGFF"
     gene            complement(579..2966)
                     /locus_tag="PLL28_09965"
     CDS             complement(579..2966)
                     /locus_tag="PLL28_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNF69691.1"
                     /translation="MNRIIKKIAVLGSGVMGSRIACHFANIGIEVLLLDIVPLQATEE
                     QLKQPAFRNKIVNDSLQAAIKQNPSPVYSQKVLKRITTGNFDDDMQRIADCDWVMEVV
                     VENLTIKQQLFEKVDKHRKQGTLVTSNTSGIPIALMSEGRSEDFRRHFCGTHFFNPPR
                     YLRLLEIIPGPDTEKSVIDFLMNYGDRYLGKTTVLCKDTPAFIANRVGVFGMMAVLKV
                     MEEMQFSIAQVEALTGPIYGRPKSATFRTADVVGIDTFVKVAQNTYAACPDDESRDIF
                     QIPAYINTLVENKWLGDKSGQGFFKKVKNAQGQSEILTLDLNTLEYQPSTKAKFAALD
                     AAKPIDNLKDRIRTLHATGDKASEFYNKLNALVFKYVSHRIPEIADELYKIDDAMRAG
                     FGWELGPFEIWDVLGVEKFVAWMEQHGTTPASWVKEMLQKGCKSFYLVENGKRKYYDA
                     RSGGYVLIPGMDAFIILDNYRAQKPVWQNSGATLHDIGDGILNLEFHTKMNVVGSEIL
                     EGIQKSIDIAEKNYRGLVIGNDAANFSAGANIAMMLMLAIEQEFDELDMAIRVFQNTV
                     ARIRFSGIPVAVAPHGLTLGGGCEMTMHADVAVAAAETYIGLVEVGAGLIPAGGGTKE
                     LALRASEKYFKGDIEIPTLQEMSLNIAMAKVATSAREAFEFGLLRNGIDKEVINGARL
                     IAEAKKQVLSLADAGYVQPKPKSDIRVLGRSALGSFYVGISSMRMSGYISEYDEKIAK
                     KVAYVISGGDLSMPTEVNEQYLLDLEREAFLSLLGERKTLERMQHILKTGKPLRN"
     gene            complement(3033..3617)
                     /locus_tag="PLL28_09970"
     CDS             complement(3033..3617)
                     /locus_tag="PLL28_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNF69692.1"
                     /translation="MPVKKIDRGELIKQALNLFRTQGYHKTTMSDIGTACGLLKGSIY
                     HYFSSKEDLMREVLEVLRNYYRDKVFIHAFNDALTAEQRLNMLGEKSIEVFVNGDGAC
                     LMANIAMETNDVVPEFRAPIRAFFDDWTNALTHIYSDKYSKEAATIFAGETICAIEGA
                     AMLMRIYNDRTYLDKAHQMITEKFKVGSKAALLK"
     gene            complement(3811..4233)
                     /locus_tag="PLL28_09975"
     CDS             complement(3811..4233)
                     /locus_tag="PLL28_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69693.1"
                     /translation="MRTSSFLIAGVATAMILLSACTKTDTFILSVSDAENIIPGSYKV
                     ATFIDIAGEGTVYENYIFQFTSNGDLTATDGTDTYTGTWSIENENDPVYDKKVTISIG
                     GNTEMDVLNHGWLVEEVTDATLYLTDDTGVESVHFVKN"
     gene            complement(4457..4882)
                     /locus_tag="PLL28_09980"
     CDS             complement(4457..4882)
                     /locus_tag="PLL28_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69694.1"
                     /translation="MKKLMTMGLVAGIVATLASCSDDSLNGITTAEAESKSPGSWKVS
                     YYFDNSDGVSHELDAYTFNIAEDGTLTVSGGGATYTGTWVVKNSDDDPNYDTEIDFSI
                     MGNSDMDKIDGSWLMGTINDTKMELLDDSGSEELHLSRQ"
     gene            complement(5093..6364)
                     /gene="purD"
                     /locus_tag="PLL28_09985"
     CDS             complement(5093..6364)
                     /gene="purD"
                     /locus_tag="PLL28_09985"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNF69695.1"
                     /translation="MNILLLGSGGREHAMAWKIAQSKQCDQLFIAPGNPGTASCGKNI
                     AVKATDFPALATVCLENKIDMVVVGPEDPLVAGVVDYFASNPAIAHIPVIGPNKQAAQ
                     LEGSKAFAKAFMIRNKVQTPTYKTFTASDSAAAHEYLKGHPMPVVLKADGLAAGKGVV
                     ICQTAEEAIVEFDQMVSGKFGVAGNTVVVEQFLTGIELTVIILTDGKTYTILPPSKDY
                     KRVGDGDTGLNTGGMGAVSPPPFATEAFLDKVKEKIIDPTMMGLKNEGIHYTGFLYFG
                     LFNSNGEPYVIEYNCRMGDPETQVIMPRIANDIIDMYKAVAQGTLASQPLITDPRPCT
                     TVILASHGYPGVFEKGYAITGIENTEQCMVFQAGTALNNNGELVTAGGRVMAVSAYGD
                     DIASALRTSYINAGRIYYESRYFRRDIGWDM"
     gene            6565..7518
                     /gene="metF"
                     /locus_tag="PLL28_09990"
     CDS             6565..7518
                     /gene="metF"
                     /locus_tag="PLL28_09990"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNF69696.1"
                     /translation="MKVTEHLNRADKTLLSFEILPPLKGKSIQNIYSVLDPLVEFKPA
                     FINVTYHRSEYIYKKRGDERFDKVFIRKRPGTVGICAAVQFKYHIDSVAHIICGGFTK
                     EETEDALIDLSYLGIDNVLLLRGDASKNERDFIPEPGGHSHAVDLVRQVVDMNRGKYL
                     EEDSIQGYKTDFNIGVAGYPEKHFESPNLKSDLKHLKEKIDAGADYIVTQMFFDNQQF
                     FAFVDRCRAEGITVPIIPGIKSITAASQIHSIPRTFHVDIPEALIDTIEKCTTAEAVK
                     KAGVAWCIEQSRELVKAGVPCIHYYTMGNVDAMRSILTEVF"
     gene            complement(7553..8287)
                     /locus_tag="PLL28_09995"
     CDS             complement(7553..8287)
                     /locus_tag="PLL28_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69697.1"
                     /translation="MRPATCLIIAISLMQHSFAQIDDVKPVNGHQRIDVNLDLGYRIE
                     RTGSNLADFVHTTGFGAGGDYSIGSASLRLVMSPYIHAQSGNDFNEMKAYYKAKGYTL
                     NSFNGGMYYSTGMLLGARYVIPTRRPSGLPSFYAALQYGFQAAHTPACDINYSTINGD
                     IISIKDDQAIALGPTWRLQLGADVLRSGDALYSIQAGVRQERAALPVDRTTLGVPGLD
                     TPGSTEIFQWNGIYAVIGLGVAFNME"
     gene            complement(8295..9551)
                     /locus_tag="PLL28_10000"
     CDS             complement(8295..9551)
                     /locus_tag="PLL28_10000"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNF69698.1"
                     /translation="MKICYCFISAVLLLCSCKEEELLTWDPYEYPEDPEPPNELIWQV
                     PLHQDTLGCLATWPQIYQNAILCSGEEGWENPVRVFAVDTSGNLMWESNAVFNENCSS
                     ISASVGTGAYQYQNMVAMLCNADPRVVNIQTGEVMWHYEVPGSGNHSPYMSGFGDQIF
                     HAYADGPNPYTKSYIARANINTGIWDTVFSIEMVDGYSADLYPPGAWVDNNGDTIICF
                     QNRQWRNPPYDGKVDLYAYNLTADSLLWMYSDIDPDGNSSVFPPVYADGKVFFRGYFH
                     VYGLDAKTGNLIWTWASPNVANDLLYTNTLVVDDKVLVKTTGPELFALDPDNGSEIWH
                     TSGFGEIPSELVAFDGMLYFSTEIDGKLNAVNIQNGSLEWTMTSPNHGQPTSSWAYFS
                     RPVTIDPLLHRLYLTDFFYLLCIQLK"
     gene            complement(9555..>10710)
                     /locus_tag="PLL28_10005"
     CDS             complement(9555..>10710)
                     /locus_tag="PLL28_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69699.1"
                     /translation="SDAILIAHSQGGIVSRRVDKMYTTGEFGFEPRTFGGMVTFGTPH
                     QGAKILNNIEDLQDWVGESCEALSAGPVAEAVESSFFLDLFLDPVDYESFTEEFCNSV
                     QNQILPLVMDDYLAGTTSAYSVGAPYLATLNTFVPEIPYVCFYGVETQPIFWHTLVHL
                     FPGREPNNTITYGEAPFGATNDETAVAFADAMIGKYYGKYTSYNALYEEYNDLLTGFD
                     MATIGCYLSVFCAVDAIIQRDEAAIIRDAYEKGYDWLINANAAWEGFIGMSELVEDGT
                     TCTCIDWGPLGDLEEDIYPAGPDGSCATEDEDTNCYTTTNYIWQTHDSDGIVVKESAA
                     ECLGQVSEGMVFTQMPGSNHLSMRNDANTKLKLIHLYDGVHGSFYKTNPR"
BASE COUNT         2946 a         2386 c         2179 g         3199 t
ORIGIN      
        1 tgaagaaacc tttgtcaata gcttctgttg cttttctatg cgagtgaaat gcgtaggcat
       61 ctgcatcttc acgagaaata ttaaattctt ttgcaacatt ttcggcagta agtcccatgc
      121 cgataagata ttcgggatgt tcagcagcaa cttcataatt taaagaagtc ttccaaccgg
      181 cagtaggcac caaactcata ctttcagtgc cgccggcgat aatacaatcg gccatgccgc
      241 ttcgaatttt tgctgtggcg atagcaattg cttctacccc acttgcacaa tatctgttga
      301 tggtaatacc ggcagttgat ttatccaatg cgcgcatggc aatttgtcgg ccaatttgca
      361 atccctgttc tgcttcgggc acagcgttgc cgactataca atcttcgatg cgcgattttt
      421 ccaattgtgg aatttgtgcc agcaactgcg taattacctg aacggcaata tcatccgggc
      481 gcataaatcg gaacatgcca cggggcgctt ttccaaccgg tgaacggagt cctgcaacta
      541 tatatgcttc tgtcataatt ttctttttat tgattgaatt aattgcgcaa tggctttccg
      601 gtttttaata tatgttgcat gcgctctaat gtttttcttt cgccaagcaa agaaaggaat
      661 gcttctcttt caaggtcgag taaatattgt tcatttactt cggtaggcat gctgaggtca
      721 ccaccgctaa ttacgtaggc aactttctta gcaatttttt catcgtattc agaaatgtat
      781 cccgacatac gcatggatga tattccgaca taaaaacttc cgagtgcaga gcgacccaat
      841 actcgaatat ctgatttggg tttaggttgc acataaccag catcagccag agacagcact
      901 tgtttttttg cttctgcaat taatcgcgca ccatttatta cttctttatc aataccattg
      961 cgcaagagtc caaactcaaa ggcttcacgt gcactagttg caacctttgc cattgcgata
     1021 ttcagactca tttcctgtag tgtaggaatt tcaatatcac ctttgaaata tttttcactt
     1081 gcgcgcaatg ccaattcctt ggtacctcca cctgcaggaa ttaatccggc gccaacttca
     1141 acaagtccga tatatgtttc tgcagcggcc acggctacat cggcatgcat agtcatttcg
     1201 cagccaccac ccagggttaa tccgtgagga gcaacggcta ccggaattcc ggaaaagcga
     1261 atgcgtgcaa ctgtattttg aaaaacgcga atggccatat cgagctcatc aaattcctgt
     1321 tcaatggcaa gcattaacat catagcaata tttgctcctg ccgaaaaatt tgctgcatca
     1381 tttccaatta ccagaccgcg ataattcttt tctgcaatgt cgattgattt ttgaatgcct
     1441 tccaaaatct cacttccaac tacattcatt ttagtatgga attcgagatt caaaatgcca
     1501 tcgccgatat catgtaaagt agctccactg ttttgccata ccggtttttg tgcacgataa
     1561 ttatcaagga tgatgaatgc atccattccc gggataagca catatcctcc ggaacgagcg
     1621 tcataatatt tgcgtttacc gttttcaacc agatagaaac ttttacatcc cttttgcaac
     1681 atttccttca cccaggaagc aggagtagtt ccgtgttgct ccatccatgc gacgaatttt
     1741 tccacgccga ggacatccca tatttcaaat ggtcccagtt cccatccaaa acctgcacgc
     1801 atggcatcat caatcttata tagttcgtca gctatttcag ggatgcgatg tgaaacatat
     1861 ttaaaaacga gtgcattcag tttattataa aattctgatg ccttatcgcc tgttgcatgt
     1921 aatgtgcgaa tacgatcttt tagattatcg ataggtttcg ccgcatccag cgctgcaaat
     1981 tttgcttttg tggagggctg atattcaagc gtatttagat cgagagttag aatttcactt
     2041 tgtccttgcg catttttcac tttcttaaaa aatccttgtc cggatttatc gcccagccat
     2101 ttgttttcaa caagggtatt gatgtacgcc ggaatttgga agatatcgcg gctttcatca
     2161 tcgggacatg cggcgtaagt attttgtgcc actttcacga aggtatctat gcccaccaca
     2221 tcggcagtgc ggaaggtggc agatttaggt cggccgtaaa ttggtcctgt aagtgcttct
     2281 acctgagcaa tagagaattg catttcttcc attactttaa gaacagccat catgccaaat
     2341 acaccaacgc gatttgcgat aaatgcggga gtgtctttac aaagcacagt ggttttccca
     2401 agataacgat caccataatt catgaggaaa tcaatcacac ttttttctgt atccgggccg
     2461 ggaattattt ccagcaaacg gagatagcgc ggcggattaa aaaaatgtgt tccacaaaaa
     2521 tggcgacgaa aatcttcgga tctgccttca ctcattaatg cgattggaat acctgaagtg
     2581 ttagacgtta ccagtgttcc ttgtttgcga tgtttatcca ccttttcaaa cagctgctgc
     2641 ttgattgtca gattttccac taccacttcc atcacccagt cgcagtcggc aatgcgctgc
     2701 atatcatcat caaagtttcc cgtggtaatt ctcttcagca ctttttgtga ataaaccgga
     2761 gatggatttt gtttaattgc ggcttgcaga ctgtcattta caattttatt tcggaaggca
     2821 ggttgcttca attgctcttc cgttgcttgt aatggcacga tatcgagcag gaggacttca
     2881 atgccaatat ttgcaaaatg acaggcaatc cgacttccca taacgccgga accgaggact
     2941 gcaatttttt tgataattct gttcataaca tttattcttc gaggagcatt tttattaatc
     3001 ttatcaaatg ctttaatgtt gtttatttta tattacttca gcaatgctgc tttactgcct
     3061 actttgaatt tctccgtaat catttgatgt gctttgtcca ggtaagtgcg gtcgttatat
     3121 atgcgcatca acatggctgc gccttcaata gcacaaattg tttcaccggc aaatatggtt
     3181 gcagcttcct tgctgtattt atctgaatag atatgcgtaa gtgcatttgt ccagtcgtcg
     3241 aagaaagcgc gaatcggagc acggaattca ggaactacat catttgtttc cattgcaata
     3301 tttgccatga ggcaagcgcc atcgccattg acaaatactt caatgctttt ttctccgagc
     3361 atattcaatc gttgttcggc ggtaagggca tcattaaatg catgaataaa tactttgtcg
     3421 cgataataat tgcggagcac ctccagcact tcgcgcatga gatcctcctt ggatgagaaa
     3481 tagtgataga tactcccctt caacagacca caagcagttc cgatatcaga catggttgtt
     3541 ttgtgataac cctgagtacg gaacaagttt agcgcttgtt ttatgagctc gcccctatca
     3601 atttttttaa ccggcatagg acaaagatat atggagattt catttaacca aacgtttgtt
     3661 tggttaaatt attataaaat gtctcggttt gtgccgaaaa aaagctctgc ctgtgggtgt
     3721 aagcgctttt acgcttgggt ttggggcaaa aaaaaccggc tttcctgtca agaaagccgg
     3781 caccttatat tgatacgttg taaatccgga tcagtttttc acaaaatgaa ccgattccac
     3841 gcctgtatca tcagtgagat acaaggttgc atcggtaact tcttctacca accagccatg
     3901 attaagcaca tccatttccg tatttccacc aattgaaata gtaacttttt tatcatacac
     3961 cggatcattt tcattctcaa tagaccaagt gccggtataa gtatcagtac catctgtagc
     4021 ggtcagatcg ccattgctcg taaattgaaa gatataattt tcgtataccg ttccttcacc
     4081 tgcaatatct atgaaggtgg ccactttgta agaacccgga attatatttt ccgcatcaga
     4141 aacggaaaga ataaatgtgt ctgtctttgt gcatgcactt agcaatatca ttgcagttgc
     4201 aacgccggca attagaaagc ttgaggttct catattcagt cagattagtt ttacaataaa
     4261 atgaaataaa tcccacatca gttgcatcta ttaattttca ataatgtcac ataattcagt
     4321 aatttacatg tctaagatat ccaatcaata aacttttcag ccctatttac ggatattaac
     4381 atgcaattta ccttgtcaca tatttgccat tggtgaaatt tttcaagatt gcgcattttt
     4441 gtggacgatt tttgaattat tgccttgaaa gatgcaattc ttcagaccct gaatcatcga
     4501 ggagttccat tttggtatcg ttgatggttc ccataagcca actgccatcg attttgtcca
     4561 tgtcactgtt tcccataatg ctgaaatcga tttcagtgtc atagttcgga tcatcatcgc
     4621 tgttctttac cacccaagtg ccggtatagg ttgcgccgcc accgctaact gttaaagtgc
     4681 catcctcggc aatattaaaa gtgtaggcat caagttcatg gctaacgcca tcgctattgt
     4741 cgaaataata ggaaaccttc catgatccgg gcgacttgct ttcagcttcg gcagtggtga
     4801 tgccatttaa actatcatcg gaacatgagg cgagcgtggc aactatccct gcaaccaagc
     4861 ccattgtcat caattttttc atacgttgtt atttgagttt attgttctgt tatgtgggat
     4921 gaaacggggt tgaagcccga atagttgcgt aaatccggcg acagaaaatc gtcgattcca
     4981 ctataaaaaa atcggaaagt caatggcatg aaaagacaca aacccttata aacaaaaggt
     5041 tccagctcaa tacacgtata gaaattgttc ggaaaaacta ctatgcaaat agttacatat
     5101 cccagccaat atcgcgtctg aagtaacgag attcgtagta gatcctgcct gcattgatat
     5161 agctggttcg cagtgctgat gcgatatcgt cgccataagc cgacactgcc atcacccttc
     5221 cgccggcggt tactaattca ccattattat tcagcgcagt ccccgcttga aagaccatac
     5281 attgttcagt attttcgata cctgtgatgg cataaccttt ttcaaacaca ccgggatatc
     5341 cgtgagaggc aaggattacc gtagtgcatg gacgaggatc tgtgatcagt ggttgtgatg
     5401 caagagtgcc ttgagccact gccttataca tgtctatgat atcattcgct atgcgcggca
     5461 tgattacttg tgtttcggga tcacccatgc ggcaattata ttcgattacg taaggttcgc
     5521 cattgctatt gaaaaggccg aagtagagaa atccggtgta atgaatacct tcatttttca
     5581 aacccatcat agtagggtca atgattttct ctttcacttt atcgaggaaa gcttctgtag
     5641 cgaatggagg tggtgaaaca gcgcccatac cacctgtatt cagcccggta tcgccatcac
     5701 ctacacgttt atagtctttt gatggtggta agatggtata tgttttacca tcagttaata
     5761 tgataaccgt aagttctatc cccgttaaaa attgttcaac cactacagtg tttcctgcca
     5821 caccgaattt tccgctcacc atttgatcaa attcaacgat tgcctcttct gcggtttgac
     5881 aaataacaac accttttccg gcagccaatc cgtcagcttt cagaacaacc ggcataggat
     5941 gtccctttaa atattcatgc gccgcagcac tatcagaagc ggtaaaagtt ttataggtcg
     6001 gcgtctgcac cttattacgt atcatgaatg ccttggcgaa ggctttgctt ccttccaatt
     6061 gggcagcttg tttattcgga ccgattacag gtatatgcgc aattgcggga tttgatgcaa
     6121 aataatcaac aactccggca accagtggat cttcaggtcc cacaaccacc atatcaattt
     6181 tgttttcgag gcacacagta gccagagcag ggaagtctgt ggccttcaca gcaatatttt
     6241 ttccgcaaga tgcagtgccg gggtttccgg gggcgataaa taattgatcg cattgtttcg
     6301 actgagcgat tttccaagcc atagcatgtt cacgacctcc ggaaccaaga agaagaatat
     6361 tcatgatgca aagatagtgg gtgagtgggt gagtggtgag tgtggttcgg aggggagctc
     6421 gactcgaaac ccacctgcta acttgcaaac ttgctaactc ttacgttcca cctctctcct
     6481 ttttcctctc tcctttttcc ttcctccttt ttccttcctc ctctattcct catcaattat
     6541 ctttgcttct cattttattt tttgatgaaa gttactgaac acctcaatcg tgccgataaa
     6601 accttgttgt cgtttgagat cttaccgcca ttgaagggta agagcataca aaatatttat
     6661 tcggtgctgg atccattggt ggagtttaag cctgcattta tcaatgtcac ctatcatcgc
     6721 agcgagtata tttataagaa gcgcggtgat gagcgttttg ataaagtatt tattcgcaag
     6781 cgtccaggaa ctgttggtat ttgtgcggca gtccagttta aatatcatat tgattctgta
     6841 gcacatatta tttgcggagg atttaccaaa gaagaaacag aggatgcatt aatcgacctg
     6901 agttatttag gtattgacaa tgtattactg ttgcgtggag atgcatcgaa gaatgaacga
     6961 gattttatac ctgaacccgg cggtcacagt cacgcagtag atcttgttcg tcaggtagtg
     7021 gatatgaatc gcggcaaata tttggaggag gattctattc aaggttacaa aacagatttc
     7081 aatattggtg ttgccggata tccggagaaa cattttgaaa gtccgaattt aaaatcagac
     7141 ctcaagcatc tgaaagaaaa aattgacgcc ggtgcagatt atattgtaac acaaatgttt
     7201 ttcgacaatc agcagttttt tgcatttgta gaccgttgtc gcgctgaagg cattactgtt
     7261 ccaattattc ccggaattaa atccataact gcagcatcac aaatacatag tattccgcgc
     7321 acctttcacg tagatattcc tgaggcgtta attgatacca ttgaaaaatg cactacagca
     7381 gaagctgtaa aaaaggccgg tgttgcctgg tgtattgaac aatcgcgcga gctagtaaaa
     7441 gcgggagtgc cttgcataca ttattacacg atgggcaatg ttgacgctat gcgttctata
     7501 ttgactgagg tattttgatt tatgttataa caataattat ttcagcattc aactactcca
     7561 tattgaatgc aacaccaagt cctatgacag catagatacc attccattga aatatttctg
     7621 ttgaaccggg cgtatcgagt ccgggcacgc cgagggtagt tctatccacc ggcaatgctg
     7681 ctctttcctg acgaacgcct gcttgaattg aatacaatgc gtctccggag cgcagcacat
     7741 ccgcgcctag ctgcaggcgc catgttggtc cgagagcgat agcctgatca tctttgatag
     7801 aaataatatc gccattgatt gttgaataat tgatatcgca tgcaggtgta tgagcagctt
     7861 gaaaaccgta ttgcaatgcg gcataaaacg aaggtaatcc tgaaggccgg cgggtaggaa
     7921 ttacatatct tgcaccaagt aacattccgg tagaatagta cattccgcca ttaaatgaat
     7981 taagggtata acccttggct ttataatatg ctttcatttc attgaaatcg ttacctgatt
     8041 gtgcatgaat atatggcgac ataaccaagc gtaatgatgc ggacccgata ctataatcgc
     8101 cgcctgcacc gaatcctgtt gtatgcacaa aatcggcgag gttgcttcct gttcgttcaa
     8161 ttcgatatcc gagatcaagg ttgacatcaa tgcgctgatg cccatttacg ggcttgacgt
     8221 catcaatctg tgcgaagctg tgttgcatga gggaaatcgc gatgatcaaa catgttgctg
     8281 gtctcatata ctgactattt tagttgaatg cacaatagat aaaaaaagtc tgtcaaatac
     8341 agtctgtgca acaggggatc aatggtaacg ggcctggaaa aatatgccca tgacgaagtt
     8401 ggttgtccgt ggttggggga tgtcatggtc cattcaagag aaccattttg tatgttcacc
     8461 gcattcagct tcccgtcaat ttcggtgcta aagtataaca tcccgtcgaa tgctacgagt
     8521 tcagatggaa tttccccgaa cccggaggta tgccaaatct cacttccatt atccggatcg
     8581 agcgcaaaca attcaggtcc cgttgttttc acaagtactt tatcatcaac aaccaatgta
     8641 ttggtatata gcagatcatt tgccacattg ggtgaagccc aagtccaaat taaatttccg
     8701 gtttttgcat ctaatccgta cacatgaaaa tatcctctga aaaacacttt accatcggcg
     8761 tacacaggag ggaatacaga actattacca tcgggatcta tatcactgta catccaaagc
     8821 aatgaatctg cagtaaggtt atatgcataa agatcgacct tcccatcata gggaggattt
     8881 ctccattggc gattttgaaa acagataatt gtatcaccgt tattgtctac ccatgcgccg
     8941 ggaggataaa gatctgcgct gtagccatct accatttcta tgctaaaaac ggtatcccaa
     9001 ataccggtat taatatttgc tcttgcgata taacttttcg tgtaaggatt aggtccatcg
     9061 gcataagcat gaaatatctg gtcgccgaat cccgacatat aaggactatg attccctgat
     9121 ccgggaactt cataatgcca catcacttca cctgtttgaa tatttaccac tcttggatct
     9181 gcattgcaga gcatggctac catattttga tattgataag ctccggtgcc gacgcttgca
     9241 gaaatgcttg agcaattttc attgaaaacg gcattgcttt cccacatcag atttccggaa
     9301 gtatctacgg caaaaacgcg caccggattt tcccagcctt cttcaccact gcaaagaatt
     9361 gcattctgat aaatttgagg ccacgtggca agacatccca atgtatcttg atgtaaaggc
     9421 acttgccaga taagttcatt tggtggttcc ggatcttcag gatattcgta tggatcccag
     9481 gtaagcagtt cctcttcctt gcaactgcaa agaagaagaa ctgctgatat gaaacaatag
     9541 catattttca tagatcaacg cgggtttgtt ttataaaaac taccatgcac gccatcatat
     9601 aaatgaataa gtttcaactt cgtatttgca tcgttgcgca tactgaggtg attacttccg
     9661 ggcatttgtg taaacaccat gccttcgctt acctgaccaa gacattccgc tgctgattct
     9721 ttcaccacaa taccatcgct gtcgtgtgtt tgccagatat aatttgttgt ggtgtaacaa
     9781 ttagtgtctt catcttcggt tgcacatgat ccatcaggac cggcgggata aatatcctcc
     9841 tccagatcgc cgagcgggcc ccagtcgatg caagtacatg ttgtgccatc ttcaacgagt
     9901 tcgctcatac caataaatcc ttcccaggct gcattagcat ttataagcca atcatatcct
     9961 ttttcatagg catcgcgaat aatcgcagcc tcatcgcgct ggatgattgc atcaacggca
    10021 caaaaaacag aaagatagca tcctattgtc gccatatcga accctgttaa cagatcatta
    10081 tattcttcat acaacgcatt gtacgaagta tactttccat agtatttacc aatcattgca
    10141 tcggcaaatg ctacagctgt ttcatcgttg gttgcaccaa aaggtgcttc accatacgtg
    10201 atggtattat tgggttcacg gcccggaaaa aggtgtacga gtgtgtgcca gaagatgggt
    10261 tgcgtttcga caccgtaaaa acaaacataa ggaatttccg gaacaaatgt attaagtgta
    10321 gctaaatatg gtgctccaac agagtatgcg ctggtggttc cggcgaggta atcatccata
    10381 accagcggca ggatttgatt ctggacactg ttacagaatt cttcggtgaa gctttcgtaa
    10441 tcgacaggat ccaggaacaa gtctaggaaa aaacttgatt caacggcttc ggcaacaggt
    10501 ccggcagaaa gtgcctcgca agattcccct acccaatctt gcaggtcttc gatattattc
    10561 agaattttcg ctccttgatg cggagttccg aaagtgacca tgccaccaaa ggtgcgcggt
    10621 tcaaatccga attcaccggt ggtgtacatt ttatccaccc tgcgagatac gattccaccc
    10681 tgactatgtg ctatcaaaat agcatctgag
//
LOCUS       DAOQHY010000115        10565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_3145_length_10565_cov_4.852997, whole genome shotgun sequence.
ACCESSION   DAOQHY010000115 DAOQHY010000000
VERSION     DAOQHY010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10565
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3145_length_10565_cov_4.852997"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1096)
                     /locus_tag="PLL28_10010"
     CDS             complement(59..1096)
                     /locus_tag="PLL28_10010"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNF69700.1"
                     /translation="MIDFLIIGGGHAGMHTAFAAMQRKCSFVLVDPFEENATNISAGI
                     INPITGRKYKPQWHIEVLLPFAERIYAAMERQLETSILHHSGIWKMHKSEEALEAWLM
                     AGKTPETMTWVSEDAEQHAWQKHLDHRFGAIQIPKAKRVDTTAMMNAFRKIYAENCMK
                     ESINYAELEVHDDHITWKGEKYRHIVFCEGSRVMQNPWFGHISLWPAKGECLIAHIPH
                     LKSNVILQKKVFIVPFGSDIYWIGASNDFTDATYDPTQAKLDELTEGLRDMLNLPYKI
                     IAHRAGLRPTMRDRTPVVGTHPLHKSLHILNGLGTKGSLLAPYYAHILLDDILHNTGI
                     PAPADVQRTFR"
     gene            complement(1111..1779)
                     /gene="mnmD"
                     /locus_tag="PLL28_10015"
     CDS             complement(1111..1779)
                     /gene="mnmD"
                     /locus_tag="PLL28_10015"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HNF69701.1"
                     /translation="MRSTLVITADGSPTIFVPELNEHYHSVHGAVQESMHVFIRAGFD
                     EAAINNASLSILEVGLGTGFNLLLTLNAVIHNQVDVTYHAIEPFPPANEILSAFTSAH
                     LEQLGEGAAYFHALHQVPWEKAAVLHERFTLIKYRDTLSDFTSNSKYHLIYFDAFAPG
                     VQPDMWKQEHFVKLFNLMHPDGILVTYCAKGDVRRAMQSAGFKVERLPGPPGKREMLR
                     ARKI"
     gene            complement(1776..2504)
                     /locus_tag="PLL28_10020"
     CDS             complement(1776..2504)
                     /locus_tag="PLL28_10020"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNF69702.1"
                     /translation="MAFFSFTNISTGDNSPQLEKHEEQKLLFLSIFMPVVFITIMWIV
                     HIFEYVTGMDLGKYGILPKHIEGLPGILFSPFIHGDWQHLISNTAPFLILSFLMIFNY
                     RKVAFKAFVFIYLAGGLLLWLTGRENYHIGASGLIYGMAFFIFFSGVFRKNLQAIALS
                     LFVVFLYGSIVWGMFPIDFKISWEGHAMGAVCGFVAAFYFRNVDRPAPIILEDDPEDD
                     DEMIPHEHEPIEITYTIVDDKPRT"
     gene            complement(2506..3123)
                     /locus_tag="PLL28_10025"
     CDS             complement(2506..3123)
                     /locus_tag="PLL28_10025"
                     /inference="COORDINATES: protein motif:HMM:NF025946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNF69703.1"
                     /translation="MIATDNDLQTKIPFALTNVYDFAGFTTLTAQMVAEGTTTGPVKS
                     ESRIDATKLNIQRINRLIKTTKLLPEWDTLFGIENLEFLAITETWCGDGAQILPVVSR
                     IADHIGVPLRIILRDEYPDIIDRYQFRNTRSIPVLIAFNRLTGEELGWWGPKPADAQK
                     IVDDAKINNIPHDDYVISLQNWYNQTKTIPLQQELLSFVQRCLSK"
     gene            3248..4309
                     /locus_tag="PLL28_10030"
     CDS             3248..4309
                     /locus_tag="PLL28_10030"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HNF69704.1"
                     /translation="MSSLRAVKYLAGYSIPITAVISMSMHGIAVFITPFYAFVFLAFL
                     EMILPPDHKNLKAMEAELAKKDKVYDILLYFHLPVQFAVLVYFLIQVNEQGLAGYEIA
                     GRIISMGICCGVIGINVGHELGHRVNKTEQRMAQLLLMSSLYMHFFIEHNKGHHKRVS
                     TPEDPSSARMHEPIYFFFVRTIIFSIQSAWQIQCQELREAGRKIISAHNTLLVFFIVE
                     TIFVLAIGFFIGWVVMSYFLIAAGIGILLLESVNYIEHYGLRRQLKSSGLYERVQPWH
                     SWNSDFVLGRVVLYELTRHSDHHYLASKKYQTLNYSEEAPQLPAGYPAMILLALIPPL
                     FFRVMHPRIKALEFQHSTV"
     gene            4319..4924
                     /gene="udk"
                     /locus_tag="PLL28_10035"
     CDS             4319..4924
                     /gene="udk"
                     /locus_tag="PLL28_10035"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF004018.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNF69705.1"
                     /translation="MFIIGIAGGSGSGKSTFVRNLVKQLDVDTVSLLMQDAYYRHMPE
                     MPEAERKQMNFDHPDAIEWELLAEHMQTLQSGNIIAQPQYSMLTCLRENETTQVKPNK
                     VLIVEGILVLTQPIVRDLMSLKIFIDADADHRFMRVVKRDMEERGRNIDQVMQRYLKT
                     VRPMHETFIEPSKKHADIIVPIGGDNHVAIDLLTGYIRSRL"
     gene            4963..5559
                     /locus_tag="PLL28_10040"
     CDS             4963..5559
                     /locus_tag="PLL28_10040"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNF69706.1"
                     /translation="MKTIICILILALPASLFAQRYPTGKGTYRAGGGGSMSISDKTDY
                     YDYKVTITPRFGYFVTDNLLTGFSMNYTMELDTAFTSAIKFTPQAKYFYKLNPSTFIL
                     ATAEFGLDRSTTYVDPKSIVDHSSVSFGPGVAYYFTRRVGFEINIMTQLYFQPDGSHN
                     NKVYAEGGFVLNVLNKNQKKKNPFKQKGDYEIKEDDDE"
     gene            complement(5564..7006)
                     /locus_tag="PLL28_10045"
     CDS             complement(5564..7006)
                     /locus_tag="PLL28_10045"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNF69707.1"
                     /translation="MALTLTKSGNPHAATIVYLIRHIDDLNTAALAPFKDLLLSKIND
                     KDNGITLFHSGMGHVVAVLLKSPADNAKVGELLEESRIKGGHICKRLNHEKVTSATVL
                     RASTSVSKEELLAFCEGVALANYQFLKYKSDKKVNSLTSLLIDDAAVDGIELDELNTV
                     IAANHIARDLGNEPVVGLNSVQLGEFAVEAGKRAGFQTEVLNKAQIQSLRMGGLLGVN
                     AGSSVPPTFSILTYKPDNATNIDPFIFVGKGVTYDTGGYSIKTGASMPGMKMDMCGAA
                     AVIGAMYAIATNKLPVYCIGLIPSTDNRINSDALVPDDVITMHDGTTIEVLNTDAEGR
                     LILGDALSYAKKYNPALVIDLATLTGAAAAITGSLGSALMGTDTTYREQLIAGGDLTY
                     ERLWEIPYWKEYADMLKSDVADMKNIGGAIGGSATAGKFLEHFTDYPWLHIDIAGPAI
                     LKDDEAYKQKGASGVGVRLLYSFIKQMAGK"
     gene            complement(7104..8483)
                     /locus_tag="PLL28_10050"
     CDS             complement(7104..8483)
                     /locus_tag="PLL28_10050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNF69708.1"
                     /translation="MYAIVDIETTGTNAGHHGITEVAIYIHDGNKVTDHWSSLINPKS
                     EIPLFIQQMTGITNEMVATAPTFEEVAAEIYHLLHPHIFVAHNAGFDYGFIKAQLAEN
                     GYDLHAKKLCTVRLSRKIIPGMRSYSLGRLCESLGIVIHDRHRAAGDALATVHLFQQL
                     LEKDHGGHIPAALKRNTKEQHLPPNMERKMLDALPSGCGVYYFHDAAGKIIYVGKAIN
                     IRSRVFGHFTYAEHDGKENALRDAVFDISYELTGNELIALLLESEEIKRKFPRFNNAQ
                     KRWDRNFCIFTFTDQKGYRHLAIERFSPKKEVLKVFPDFLSARGYLNEILQTFELCGK
                     MCHLQTVKGACYNHEIGLCSGACIGLESPGDYNLRVEAAISNFSEEDHTFFIFGKGRQ
                     DGEQSVICVEKGHYLGFGFFDPENAPQDPEFLKAAIKWRPDTPDVQRILMGWMERNRD
                     NKQYRIVAF"
     gene            8700..9446
                     /locus_tag="PLL28_10055"
     CDS             8700..9446
                     /locus_tag="PLL28_10055"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNF69709.1"
                     /translation="MGMIIRFLKGLYTMYTAIIFILLLLITFPFFMVLSLWKDKALLP
                     MLWLCRFIALGLMIFCGIIYRFHDRNKYERNKAYVLIANHRSNLDAPVAAISGKGRVR
                     YLAKKELLKVPVMGQIFAVMSVYVDRSSPQDRRKSVERLKECVKSGDSIFLFPEGTRN
                     KTDQPMIDFKDGAFTIAVQTQTPILPMLYINTDTLMPNSKPLMRPGIIDVYYLPPIEV
                     AGLTDADIPALKQRTRDMLMLRYLELSGDK"
     gene            9495..>10565
                     /locus_tag="PLL28_10060"
     CDS             9495..>10565
                     /locus_tag="PLL28_10060"
                     /inference="COORDINATES: protein motif:HMM:NF027971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNF69710.1"
                     /translation="MQLQFCGAAGTVTGSAHLLTLDDGTKILLDCGLYQGNEPEYANF
                     NERFLFRPDEIDIVVLSHAHIDHCGRLPKLVKDGFRGRIYATHATRDLTAILLLDSAH
                     IQDRESSFTNRRRKDDHEDEPLYDAKDVADTMELFRTVGYNKWFGIHPDVEVVYKDAG
                     HILGSATVTLRIKRHGMRDYLLGFTADIGRPDRPILRDPVPMDACDFLICESTYGDRL
                     HEEAPQEKEHFLRVIQQTCVTQKGKLIIPAFSVGRTQEIVYMLDQLVRENRLPDVPVY
                     VDSPLAVNATRVFEMHPECFDKEILDYMALDPNPFGFEKLKYTRSVEESKAINNKKGC
                     IVISASGMINAGRVKHHVFNAIE"
BASE COUNT         2964 a         2154 c         2326 g         3121 t
ORIGIN      
        1 ccggcttctt catcaggtaa aaataattct tcacccccgc ctcaacaaat aaggtttgtt
       61 aacggaaagt gcgttgcaca tcggctggtg cgggaattcc tgtgttatga agaatatcat
      121 ccaacagaat atgtgcataa taaggtgcca gaaggcttcc ttttgttccc aggccattga
      181 gaatatgcag gcttttgtga agtggatgtg tgccaacaac aggggttctg tcgcgcatgg
      241 ttggccgcaa cccggctctg tgtgcaatga ttttgtaagg aagattgagc atatcgcgta
      301 acccttcagt caattcatcc agtttggctt gagtaggatc atatgtggca tccgtaaaat
      361 cattgctggc acctatccaa tagatatctg agccgaatgg tacaataaac accttttttt
      421 ggagtatcac attcgatttc aaatgtggaa tatgtgctat caggcattca cccttagcag
      481 gccataaact gatatgtcca aaccatggat tttgcattac acgggagcct tcacaaaata
      541 caatatgcct atatttttca cctttccatg tgatatggtc atcatggact tccaattcag
      601 catagttaat tgattctttc atacaatttt cagcatatat cttccgaaaa gcattcatca
      661 ttgcagtagt atctacgcgc tttgctttcg gaatttgtat ggccccaaat cggtgatcca
      721 ggtgtttctg ccatgcatgt tgctcagcat cttcactcac ccaagtcatt gtttcaggtg
      781 ttttccctgc catcagccag gcttcaaggg cttcttcact tttatgcatt ttccatatgc
      841 cggaatgatg caaaatactt gtttccaatt gcctctccat ggcagcatat atgcgttctg
      901 caaatggcaa taatacttct atatgccatt gcggtttata ttttcttccg gtaataggat
      961 ttattatacc ggctgaaata tttgtagcgt tttcttcaaa agggtcaaca agcacaaatg
     1021 aacatttacg ttgcattgct gcaaacgctg tatgcatacc ggcatgaccg ccacctatga
     1081 tgagaaaatc aatcacaggt caaaaataaa tcagattttt ctcgcacgca gcatttcgcg
     1141 ttttcccgga ggtccgggta gccgttctac tttgaatcct gccgattgca tagctctgcg
     1201 aacatctcct ttagcacaat aggttactaa aataccgtcg ggatgcatga ggttgaacag
     1261 tttgacgaaa tgttcttgct tccacatgtc gggctgcact ccgggagcaa aggcgtcgaa
     1321 atatatgaga tgatatttgc tattggaggt aaaatccgac agggtgtcac gatattttat
     1381 caaggtaaac cgttcgtgta atactgccgc tttctcccaa gggacttggt gtaatgcatg
     1441 aaaatacgct gcaccttcac cgagttgttc taaatgtgca gaggtaaatg ctgaaagtat
     1501 ttcattggcg ggaggaaatg gctctatggc gtgataagtt acatcaacct gattatgtat
     1561 gacagcattt aatgtcagca gcaaattaaa tcctgtacct aggcctactt cgagtatgga
     1621 aagcgatgca ttatttattg cagcttcatc aaatccggcg cgaataaata catgcataga
     1681 ttcctgaaca gcgccgtgca ctgaatgata atgttcattt aattcaggaa caaaaattgt
     1741 tggtgatcca tcggccgtaa tgacaagagt agacctcatg ttcggggttt atcgtctact
     1801 atggtatatg taatttcaat gggctcatgt tcatgaggta tcatttcatc atcatcttca
     1861 ggatcgtctt ctaaaataat cggtgccggt ctgtcgacat ttctaaaata gaaggctgct
     1921 acaaatccgc atacagcacc catcgcatgg ccttcccacg atattttaaa atcgatagga
     1981 aacattcccc aaacaatact gccatataaa aacaccacaa ataaggagag cgctattgcc
     2041 tgcaaatttt tacgaaatac gccactgaag aaaatgaaaa atgccatacc ataaattaaa
     2101 ccgctggcac caatatgata attctctcga cctgtaagcc acagcaataa tccaccggca
     2161 agataaatga atacaaatgc tttaaatgct acctttcgat aattgaagat catcagaaat
     2221 gacaggatca gaaaaggtgc agtattgctg atgagatgtt gccaatcgcc atggatgaaa
     2281 ggtgaaaaca atataccggg caagccttca atatgttttg gcaaaattcc atacttgccg
     2341 aggtccattc ctgtcacata ctcaaagatg tgaactatcc acatgattgt tataaaaaca
     2401 acaggcatga aaatgctgag gaatagcaat ttctgttctt catgcttttc cagttgcgga
     2461 ctattgtcgc ctgtagatat attcgtaaat gagaaaaagg ccatattact ttgacagaca
     2521 acgctgaaca aagctcagca gttcctgttg caaaggaatt gttttagttt gattatacca
     2581 gttctgtaaa gatatgacgt aatcgtcatg tggtatattg ttgattttgg cgtcatcaac
     2641 aattttctga gcatcggcag gctttggtcc ccaccagcct aattcttcgc cggtaagcct
     2701 gttaaaggct atcagaactg gaatggaacg cgtatttcgg aattggtacc ggtcgatgat
     2761 gtccggatat tcatcgcgaa ggatgatgcg aaggggcaca ccgatatggt cggcaatgcg
     2821 acttacaaca ggcaggattt gagcgccatc gccacaccag gtttccgtaa tagccaggaa
     2881 ctcgagattt tcaatgccaa aaagggtgtc ccattcagga agcagcttcg tggtttttat
     2941 caggcggtta atgcgttgga tattgagttt agtggcatca atgcgtgatt cgctcttcac
     3001 aggacccgtt gtagtgcctt cagcaaccat ttgtgcagtc agggtcgtaa agccggcaaa
     3061 gtcatacaca tttgtcagcg cgaaagggat ctttgtttgt aggtcgttat ctgtggcaat
     3121 cataatgcgt tccggttagt agaacatcac aaaggtgcaa agcgttcacg ggcgaaatgg
     3181 tgatgtaggt cacagagtgc ctttggtcac ataagcctat tcacatagat tttgataatt
     3241 ttgagcgatg tcatctttgc gtgcagtaaa atatcttgcc ggttacagca tcccgatcac
     3301 agcagtgatt tcgatgagca tgcatggcat tgctgtattt attaccccat tttatgcttt
     3361 tgttttcctg gcttttttgg aaatgatatt gcctccagat cacaaaaatc taaaggctat
     3421 ggaggctgaa ttggcaaaga aggataaggt gtatgacatc ctgttgtatt ttcatttacc
     3481 ggttcaattt gcagtgcttg tttacttttt aatccaagtg aatgagcaag gtttggcagg
     3541 atatgaaatt gcagggcgca tcattagtat gggcatttgt tgtggggtga tagggatcaa
     3601 tgttgggcac gaattaggcc atcgagtaaa taaaaccgag cagcgcatgg cacaattgtt
     3661 attgatgtcg tcgctatata tgcacttttt tattgagcac aataaaggtc accacaagcg
     3721 cgtatcaact ccggaagatc cttctagtgc gcgcatgcat gagccgattt acttcttttt
     3781 cgttcgcact attatttttt caattcaaag tgcctggcaa attcagtgtc aggaacttcg
     3841 ggaggcggga aggaaaatta tttcggcaca caacacctta ttagtttttt ttattgttga
     3901 aacgatattt gtacttgcca tcggattttt cataggttgg gtggtgatga gctattttct
     3961 gatagctgca ggcattggta tattattatt agaatcagtg aattatattg agcattatgg
     4021 gctgcgtcgt caattaaaat cttcgggttt atacgagcgt gtgcaaccat ggcattcatg
     4081 gaactcagat tttgtgttag gaagggtagt gttgtatgaa ttgactcgcc attcggatca
     4141 ccattatctt gcatcaaaaa aatatcagac tttaaactat agtgaggagg cccctcaatt
     4201 acctgccggt tatccggcaa tgattttgct tgcattgata ccgccattat tttttcgtgt
     4261 tatgcatcca cgaatcaaag cattggaatt tcaacactca acagtataaa tagttactat
     4321 gttcatcatt ggtattgccg gcgggtccgg ttccggaaaa tctacatttg tgcgcaatct
     4381 ggtcaaacaa ctggatgttg atacagtttc gctattgatg caggatgcct actacagaca
     4441 catgcctgaa atgccggaag cagaacgcaa gcaaatgaac ttcgatcatc ctgatgcgat
     4501 agaatgggaa ctattggcag agcatatgca gactttgcaa tccggaaata tcatcgcgca
     4561 gccgcaatac agtatgctta cttgtttacg cgagaatgaa actacgcagg tgaagccaaa
     4621 taaggtgctt attgttgaag gcattctggt gcttactcaa cccattgttc gcgatttgat
     4681 gtccctcaaa atatttattg atgcagatgc tgatcacagg ttcatgcgcg tagtaaagcg
     4741 cgatatggaa gaacgcggac ggaatataga ccaggtgatg cagcgttatc tcaaaacggt
     4801 acgtcctatg catgagacct ttattgaacc aagcaaaaaa cacgccgata ttattgtacc
     4861 tattggtggc gataaccatg ttgccattga tctattgaca gggtatatca ggtcgcgatt
     4921 gtagaagtct tattcaattt ggtatcttta attcccgcta tcatgaaaac aataatttgc
     4981 atactaattc tagccttgcc tgcttcgctg tttgcacagc gataccctac ggggaaaggt
     5041 acttaccggg caggtggggg aggttccatg tcaatttctg acaagacaga ttattatgat
     5101 tacaaggtaa ccattacccc gcgttttgga tattttgtta cagataattt gctcaccggt
     5161 ttcagtatga attacacgat ggaactggat acggcattta cctctgccat taaattcaca
     5221 ccacaagcca agtattttta caagctcaat ccgagcacct ttattttggc aactgcggaa
     5281 ttcgggctcg acagatcaac aacatatgtt gatccgaaat caattgttga ccatagcagt
     5341 gtgagttttg gtccgggtgt tgcctattat ttcacaagga gggtagggtt tgagattaac
     5401 atcatgactc aattatattt ccaacccgat ggttcgcata ataataaggt gtacgcagaa
     5461 ggaggatttg tattgaatgt gctcaataaa aatcaaaaga aaaagaatcc tttcaaacag
     5521 aagggagatt atgaaataaa agaagatgat gatgaatgat gcatcacttc cctgccattt
     5581 gtttaataaa ggaatataac agcctcacgc caactccact tgcacctttt tgcttatagg
     5641 cttcatcatc ctttagaata gccggacctg caatgtcgat atgcagccaa ggataatcgg
     5701 taaaatgttc aagaaatttc ccggcagtag cgcttccacc tattgcaccg ccaatatttt
     5761 tcatgtcggc aacatcactt ttcagcatgt cggcatattc tttccaataa ggtatttccc
     5821 agagtctttc ataggtaaga tcgccacctg caattaattg ttcgcgatag gtagtatccg
     5881 ttcccatcaa ggcgctgccg agcgaaccgg ttatagcagc tgctgcacct gtaagtgttg
     5941 caagatcgat tacgagtgca ggattatatt ttttagcata gctgagtgca tcaccaagga
     6001 tcaagcgacc ttcagcatct gtgtttaaca cttcaatggt agtgccatcg tgcatggtaa
     6061 tcacgtcgtc aggcactaat gcgtctgaat taatgcgatt gtctgttgac gggatcaatc
     6121 caatgcaata aacaggcaat ttatttgtgg cgatggcata catggctccg atcacagcgg
     6181 cagcgccaca catatccatt ttcatgcccg gcatagaagc gcctgtttta atactatagc
     6241 caccggtatc gtaggtaaca cctttgccca caaaaatgaa tgggtcgata ttagtagcgt
     6301 tatcaggttt atatgtaagg atagagaatg taggaggcac ggagctaccg gcatttacac
     6361 ccaataatcc gcccatgcga agcgattgaa tctgagcttt attcagcacc tcagtctgaa
     6421 aaccggcgcg tttcccggct tcaacagcaa attcgccaag ctgaacggaa ttcagaccaa
     6481 caacaggctc gttaccaagg tcacgggcaa tatggtttgc agcaataacg gtattcagtt
     6541 catcaagctc aatgccatcc actgcggcat catcaataag caaagaggtg agggaattta
     6601 cttttttgtc tgatttgtac ttcagaaact ggtaattagc caacgcaacc ccttcacaaa
     6661 aggccaacag ttcctccttt gagacggagg ttgaagccct taagacagtc gccgatgtca
     6721 ctttttcgtg atttaaacgc ttgcaaatgt gaccgccttt tatcctcgat tcctcaagta
     6781 gttctcctac tttcgcgtta tctgcaggtg atttcaataa aactgccact acatgcccca
     6841 tgccggaatg aaacagggta atgccgttgt ccttgtcatt gatctttgaa agtagtagat
     6901 ccttgaaagg agccagtgct gcggtattca gatcgtcgat gtgacgaatc aggtaaacga
     6961 tcgttgccgc gtgcgggttg ccggattttg taagagtaag tgccatcgct cgggattctt
     7021 gtcaaacaca gaaccacaaa ggtaaggcta aaatgcctaa attcaggatt taaaatatat
     7081 gttttgtgac atgacagatt gcctcaaaat gcgacaatac ggtattgttt gttgtcacga
     7141 tttcgctcca tccagcccat taatatacgt tgcacatcgg gggtgtcggg tctccatttg
     7201 atggcagctt tgaggaattc agggtcctga ggggcgtttt cagggtcgaa aaagccaaat
     7261 cctaagtaat gccccttctc cacacaaatt acactctgct ctccatcttg acgccctttg
     7321 ccgaaaatga agaaagtatg gtcttcttca ctgaaattgg aaattgcggc ttccactcta
     7381 aggttgtaat cgccgggact ttccaatccg atacaggcac ctgaacaaag gcctatttcg
     7441 tgattgtaac aagctccttt gactgtttgg aggtgacaca ttttaccgca taattcaaaa
     7501 gtctgcaata tttcattcaa gtagcctctc gcactcagga aatcgggaaa tactttcaat
     7561 acttcctttt taggggaaaa tcgttcaata gcaagatgtc gatatccctt ttgatcggta
     7621 aaggtgaaaa tacagaaatt gcgatcccat cttttttgtg cattgttaaa tcgcgggaat
     7681 ttgcgtttta tctcttcact ttctaataac agtgctataa gttcgttgcc tgtaagttca
     7741 taactgatgt caaaaacggc atcgcgaagc gcattctcct ttccatcgtg ttcggcataa
     7801 gtaaaatgtc cgaatacgcg acttcgaata ttaatggcct ttcccacata aattatttta
     7861 ccggcggcat catgaaaata atatacaccg caacctgatg gcaaggcgtc caacattttt
     7921 ctttccatgt tggggggaag gtgctgttct ttggtattgc gtttcagtgc tgccggaatg
     7981 tgacctccat gatccttttc caggagttgt tgaaataaat gcactgttgc taaagcgtcg
     8041 cctgcagctc gatgtctatc gtgaatgacg ataccaagcg attcgcacag tcgccccaga
     8101 gaataacttc gcattccggg aataatcttg cgactaagac gaacggtgca tagttttttt
     8161 gcatggagat cgtaaccatt ttcagccagt tgcgctttta taaaaccata gtcgaagccg
     8221 gcgttatgtg ctacgaaaat atgcggatga aggagatgat aaatttctgc ggctacctct
     8281 tcaaaagttg gtgcagtagc taccatttca ttggtaatac ctgtcatctg ctgaataaac
     8341 agagggatct ccgatttggg attgatgagt gaactccaat gatcagttac tttattgccg
     8401 tcatgaatat aaatcgccac ctccgtaatg ccatgatgtc cggcattggt tccggtggtt
     8461 tctatatcga caatggcata cattgcctaa aggtaagatg ctgtctgcag aaaatgcgat
     8521 aagatgcaaa cgctgtgatg tgtaaatttg tttttcagga tattcacttg gcgtatcggc
     8581 ataccttgaa tcagcctgaa ttgagtgatg tcagcatttg tttttgagtg gaattgcttt
     8641 cctcttgtca gcaggtttga aggtgcttat acctccccaa taatcattac cttagtccca
     8701 tgggaatgat cattcgattc ttgaaggggt tgtataccat gtatacggca ataattttca
     8761 tcttactgtt gctgatcaca tttccatttt tcatggtgct ttctttatgg aaagataaag
     8821 cattgcttcc catgttatgg ttgtgccgat ttattgcctt gggtttaatg atattttgtg
     8881 gcattattta tcgctttcat gatcgcaaca agtacgaaag gaataaagct tatgtgttga
     8941 ttgccaatca ccgttcgaat cttgacgcgc ctgttgctgc tatttcgggt aaaggacgtg
     9001 tgcgttattt ggcgaaaaaa gaattgctga aggtgccggt gatgggacag atattcgccg
     9061 tgatgtcggt ttatgtagat cgaagtagtc cgcaagaccg acgcaaaagt gttgagcgac
     9121 tgaaggaatg tgtaaaatcg ggcgacagta tttttctttt tcctgaagga acaaggaata
     9181 aaacagatca acctatgatc gattttaagg atggcgcatt taccattgca gttcaaacgc
     9241 agacaccaat cttgcccatg ttatatatca acacggatac attgatgcca aatagtaagc
     9301 cattgatgcg acccgggatt attgacgtgt attatttgcc tcccatagaa gttgcagggc
     9361 ttacagacgc tgatattcct gctttaaagc aaagaacaag ggatatgttg atgttgcgtt
     9421 atcttgaact gagtggcgat aaataatttg gaagataaac ttcagcaatt aactttacca
     9481 tacaaacacg aaatatgcaa ttacaatttt gcggggctgc cggaacggtt acaggaagtg
     9541 cacatttgct tacacttgat gatgggacta agattctttt ggattgcgga ttatatcaag
     9601 gcaatgaacc tgagtatgcg aattttaatg agcgattttt atttcgccct gatgaaatag
     9661 atatcgtagt attatcgcat gcacatattg atcactgtgg caggttgccc aagttggtaa
     9721 aggatggttt tcgtggaagg atttatgcca cacatgccac cagagatctg actgcaatat
     9781 tgttgttgga cagtgcgcac attcaggaca gagaaagcag ctttacaaat cgccgtcgca
     9841 aggatgatca tgaagatgag cccttgtatg atgctaaaga tgttgcagat acaatggaat
     9901 tatttcgcac cgtgggatat aacaaatggt tcggtataca tcctgatgta gaagttgtat
     9961 ataaagatgc cggtcatatt ctcggtagtg caactgttac attgcgtata aagcgacacg
    10021 gcatgcgtga ttatttactc ggcttcactg cagatatcgg tcgtccggat cgtccaattt
    10081 tgcgagatcc cgtgccaatg gatgcctgcg attttttaat ttgtgaatct acttacggtg
    10141 atcgtttaca tgaggaagca ccgcaagaaa aagaacattt cctgcgcgtg attcagcaaa
    10201 cttgcgtaac acaaaagggt aagttaatta tacctgcatt ttcagtgggt cgtacgcaag
    10261 agatcgtgta tatgctcgat cagttagtaa gggagaatag attgcctgat gtgccggtat
    10321 atgtcgattc acctttggct gttaatgcaa cgcgtgtttt cgagatgcat ccggaatgtt
    10381 ttgacaaaga aattctggat tatatggctt tagatcctaa cccatttgga tttgagaaat
    10441 taaaatatac gcgatcagta gaggagtcga aagcgatcaa caataaaaaa ggatgtatag
    10501 taatcagtgc ttcaggtatg attaatgcgg ggcgtgtaaa gcatcatgta tttaatgcca
    10561 ttgaa
//
LOCUS       DAOQHY010000116        10494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_3176_length_10494_cov_5.091196, whole genome shotgun sequence.
ACCESSION   DAOQHY010000116 DAOQHY010000000
VERSION     DAOQHY010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10494
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3176_length_10494_cov_5.091196"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..1971)
                     /gene="rpsA"
                     /locus_tag="PLL28_10065"
     CDS             complement(70..1971)
                     /gene="rpsA"
                     /locus_tag="PLL28_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNF69711.1"
                     /translation="MESSNSENTNTTETVVETVKKAPQTQSAHDDFDWNVDKRNRGTY
                     AQDTSAKMEAEYEGTFRTLNDAEIIMATIVALTDTDVILNVGYKSDGLVSRTEFRDLD
                     ELKVGMEVEVYVVSKEDGKGQLNLSRKSAKMMKAWEQIVDAYKNETIVTGNIISKTKG
                     GLIVEVFGIETFLPGSQIDVKPITDYDGFVGKKMEFKVVKINETIKNAVVSHKALIES
                     DIESQRQEIIGKLEKGQVLEGTIKNITDFGAFIDLGGVDGLLYITDISWGRINHPSEV
                     LHLNQKLNVVVLDFDDNKKRISLGLKQLTPHPWEVLDASIQVGSVVKGKIVNIEDYGA
                     FLEILPGVEGLIHVSEITWSNQPMNSKEFFTIGNEYEAKVVTLDREERKMSLSLKQLG
                     EDPWKSAKDKYPIGSRHSGLVKNLTNYGVFVELDENIGGMVHISDLSWIKRLNHPSEF
                     VKVGQTLDVVILDINEEDRKMSLGHKQIEEDPWDTFEQVFPIGSVHEGTIIRKDEKGA
                     MVSLPYGLEGYAPHKHLEKVDGTSAHADEVLPFKVIEFDRNDKRILVSHTKFVKETVE
                     EEKQQVKKERVRDEKKTKTQLSKVQKNVEKTTLGELDVLSQLKEQMAKDSKDAAKSNN
                     SDAEENTEA"
     gene            complement(2388..2867)
                     /locus_tag="PLL28_10070"
     CDS             complement(2388..2867)
                     /locus_tag="PLL28_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69712.1"
                     /translation="MKSFILLFIAVIGMQSIDAQRVARYTSESGSVYPDVSLLNLVAM
                     SDMNLGDWERNLKLISKLRDDFGDGGISYTVEAKTGTADGMVFITKNADAIELVHTVG
                     TNGKSIFTDFLKEMPTYYVQESEGHQVYSYTSFTKVTYVILVKVTPEQEFVRIYVNK"
     gene            complement(2927..3616)
                     /gene="cmk"
                     /locus_tag="PLL28_10075"
     CDS             complement(2927..3616)
                     /gene="cmk"
                     /locus_tag="PLL28_10075"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNF69713.1"
                     /translation="MKKIIIAIDGYAGCGKSTLARQLALKLGYTFLDTGAMYRAITLY
                     LLNNHINWHDEQALSNVFSQVKIHIATDQNGRTQTRLNGVLVEEEIRSMAVSNKVSEV
                     AAVSSIRRFLVAQQQQIGSEGGVVMDGRDIGTVVFPHAELKLFITASMEVRVARRLAE
                     LQAVGATVSADDIRRNLEKRDREETTRSDSPLKQAEDAILIDNSDLTPEAQLQLALDF
                     AWKRINPTPKA"
     gene            complement(3724..4566)
                     /locus_tag="PLL28_10080"
     CDS             complement(3724..4566)
                     /locus_tag="PLL28_10080"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HNF69714.1"
                     /translation="MKKLLIITVAVTAAVAFSSCAKDRVYGCTDPFSINYNPNATDDD
                     GGCFAPSSQKRALMGDFTATWCPYCGQWGAPEFEEAINLSGANGIPLGIHNTDEITTN
                     ESTTLTDYYSAEGIVGGFPTLYVWNQASFSDGVPMAGAVSAEVGAGPAVAGAVAGFTD
                     NGSSMTINVSAQIFADVTGDYYVAAYLMENGLVYTQQIAGADPDPNFVHNHVIRASSN
                     GSAWGEQFVFGSASNGVVWTKSYQIEKNDAWKSENMYMVAIVWKYDSAEDKYTFVNAQ
                     ETHL"
     gene            4731..6572
                     /locus_tag="PLL28_10085"
     CDS             4731..6572
                     /locus_tag="PLL28_10085"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNF69715.1"
                     /translation="MRLLKSLLTLTGLIFSLCVYAQPNIELETFATGLSNPVDIANAG
                     DDRMFVVERTGKIRIVEADGTVHPDYFLDIHTQIESGYQEQGLLGLAFHPDYAVNGYF
                     YVYYTDTDGNTVVSRFSVSAGDPDYADAGSELVLLTENQPFLNHNGGCIKFGGDGYLY
                     IGLGDGGSAGDPGNRAQNTDLFLGKMLRIDVDGGTPYAIPDDNPFATATDTLPEIWAI
                     GLRNPWRFSFDALTKNLWIGDVGQNLYEEVDVELAGDGGRNYGWRCYEGLHEFNTAGC
                     DADTAEYIFPVLEYNHNATTGGFSITGGFVYRGSMYPGMYGYYITADYVSGNWWWIYA
                     DGGAPYASLRMDDIQTSISCFGEDMNGELYCANLSSGIIYHIKDACGDFMAAAEVTDY
                     TCGVTDGAVDITITAGTAPYTYEWSSGETTEDISGITEGGTFTLTVTDASGCTRMITA
                     TVEEIPGFIAVLNEDGGVLTATPGTSWQWYLNGEIIDGATSQSYTPTESGDYSCLVTD
                     ANGCSSMTPAFNVFVEITTAQWNIDFSIHPNPASDVIYLQITDAPCATNWYIADMTGN
                     VVWRSETIGKGNQSIAISLTSLANGIYLLRSDVGSVGSRVMKVSGEW"
     gene            complement(6642..7475)
                     /locus_tag="PLL28_10090"
     CDS             complement(6642..7475)
                     /locus_tag="PLL28_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69716.1"
                     /translation="MKRIILALCSLAISASAIAQVPGYMGKRLQIYASVEPTFDFRYI
                     TDGLDYYLQQPEVTIPTEYGNYGIGVTPGVGIEYTLNNTFVMGADVKWKQAKAQLHMY
                     YNTASNPDIPYGYLGEGKISGMLTGFYFKIYSHRKHGYIAPIGRYHQFEILTGKFSVQ
                     ESGYSSTDFYAVDYQEYYSNLSLQASELTPVASLSQLNFDPHRISYLRYVFGGETVLF
                     DKVPADIGIQFAMPLSFVQYGDYDNGIDKGGYDYAYTVRKSMNGAMAVGIVMRFGIFA
                     I"
     gene            complement(7472..9688)
                     /locus_tag="PLL28_10095"
     CDS             complement(7472..9688)
                     /locus_tag="PLL28_10095"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNF69717.1"
                     /translation="MRIKSIILIYTLLLTNILVAQNFDFNNFQTLRSSGTVPDDFTEK
                     SSVKFQRDKSEIESDSKREQKDMENFLLASNYEIEDMLHSGRIVFGDPITNYLNDIKS
                     EILKGDPQLAKSIRIYTLLSNDVNAFTFDNGIVVVTTGLVSQVQNEAQLAYILCHEFV
                     HFTEKHAITSYVENRRVDRGAGVYASLGRDEEDLVKFSYAKDQESEADTKGLDLFKTT
                     NYSYDAISGVFDVLLYSYLPINEIVFPKDYFNEGMYKLPDNAFIDTINQITAVEDYDD
                     AKSTHPNIKKRKSDVLGDIPATSDASRKLNILGEARFNEIQQISRFQGCELYLIDVHY
                     EDALYQAFVLQQDFPDNPYLKRVIAQALYGWTIYKNAGTTPTGHIYYKKVEGESQQVF
                     HLFSKLNAKEFNILALKYAWDVHLAEPDNKQMTKICQHLGKQLRNEHDIDYDNFFTET
                     EVKKFIDAMNAKNDSTAVQPVDSVGKTNDTKTSKYDKIKKEKNTIDEKAPYWRFAFAS
                     VDHSAEFKALFEEEEELVATTSKKKKKSEFNLGLKELVIVDPVYISIDERSNNPVQYE
                     AAEDAKLNLKDKVKASADELNLKVEYLDHTNIDDRDVETFNEMAVLSRWIEEKLTHID
                     EDIFIETSTNDELQEIAKSLDIDHFAWMGIVSFTESEEYVGAKIFLCLYLPLAPFLIY
                     DLVTPDRNTFYFALVANAETGKLEMQYFNSSTLNDSNAAQSSNIYYILQQIKGRKK"
     gene            complement(9700..10323)
                     /locus_tag="PLL28_10100"
     CDS             complement(9700..10323)
                     /locus_tag="PLL28_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69718.1"
                     /translation="MTSLLLYKQLFVYLIPFNVLIGEYNLGMQYRIGEEWAVDLNLGY
                     VDDTEGALTAELYQDILQSGVFYYHGPLVKVSALSLVPRGPNPLKTDYNQLEFACRFL
                     RYDNLDFEDENEPGKLFNISEQMKAVSLSWKAGYIIVPRNTFEMNGFVGFGIQTRFKN
                     TIVNSYGTNYQSNVFILNDVSSSIQFVPLFHAGIKIGLKTPLKESSQ"
BASE COUNT         2824 a         2243 c         2216 g         3211 t
ORIGIN      
        1 tagccccccc ttgctaccta aaacaaaaaa ccctgctttt gagggcaggg ctttgttgaa
       61 gaatgattat caggcttcag tgttttcctc agcgtcgctg ttgtttgatt tagcggcgtc
      121 tttagaatct ttagccattt gctctttcaa ttgagaaagt acatcgagtt caccaagggt
      181 ggttttctca acatttttct gcactttaga gagctgagtt tttgttttct tctcgtcgcg
      241 aacgcgttcc tttttaacct gttgtttttc ttcttcaaca gtttctttca caaacttagt
      301 atgtgaaaca aggatacgtt tatcgttgcg atcaaattcg attactttga aaggcaatac
      361 ttcatctgca tgtgctgaag tgccgtcaac tttctcgagg tgtttatgcg gagcatagcc
      421 ttcgagtccg tatggcaatg ataccatggc acctttttca tctttgcgga tgatggttcc
      481 ttcatgcaca gagccgatag ggaatacttg ttcgaaagta tcccatggat cttcttcgat
      541 ttgtttgtga cccagcgaca ttttgcgatc ttcttcgttg atatcaagaa ttaccacatc
      601 aagtgtttgt cccactttca caaattctga agggtggttc aaacgtttga tccagcttaa
      661 atcgctgatg tgtaccatac caccgatgtt ctcgtcgagt tcaacgaata caccgtagtt
      721 agtaagattt ttcaccagac cgctgtgacg gcttccaata ggatatttat ccttcgcgct
      781 cttccaagga tcttcaccga gttgtttcag cgacagtgac atcttgcgct cttcgcgatc
      841 gagtgttact actttcgctt catattcatt gccaatggta aagaattctt tgctattcat
      901 tggctggttg ctccaagtaa tttctgatac gtgaatcaaa ccttcaacac ccggcaatat
      961 ttcgaggaat gcaccgtaat cttcaatgtt tacgatttta cctttcacta cgctgcccac
     1021 ttgaatggat gcatcaagaa cttcccacgg atgtggtgta agttgcttaa gaccaagtga
     1081 gatacgtttt ttgttgtcgt cgaagtccag tacaacaaca ttgagttttt ggttgaggtg
     1141 caatacttcg cttgggtggt tgatacgtcc ccatgaaata tctgtgatat acaacaatcc
     1201 gtcaacacca cccaaatcga tgaatgcacc gaagtctgtg atatttttga ttgttccttc
     1261 aagcacctga cctttctcta gtttaccgat gatttcctga cgttgtgatt cgatatcact
     1321 ttcgataagt gctttgtgag aaacaacggc attcttaatg gtctcattaa ttttaaccac
     1381 tttgaattcc attttctttc caacaaatcc atcataatct gtgataggct taacgtcgat
     1441 ttgtgatccc ggaaggaatg tttcaatacc gaatacttca acaataagac cgcctttggt
     1501 tttgctgatg atgttaccgg taacaatcgt ttcgtttttg taagcgtcaa cgatttgttc
     1561 ccaagccttc atcattttcg cagatttgcg gctgaggttg agctggcctt ttccatcttc
     1621 tttgctcact acatatactt ccacttccat acctactttt aattcgtcga ggtcgcggaa
     1681 ttctgtacgt gaaactaaac cgtcgctctt gtaacctacg ttgagaatta catctgtgtc
     1741 ggtaagtgca acgatggttg ccatgatgat ctctgcatca ttcaatgtgc ggaaagtgcc
     1801 ttcgtattcc gcttccattt ttgcgctggt atcttgcgca taagtgccgc ggttacgttt
     1861 gtctacattc cagtcaaaat cgtcgtgagc agattgtgtt tgaggggctt tttttaccgt
     1921 ttcaactacc gtttcagtag tgttggtatt ttcggagttt gaggactcca tgatttcttg
     1981 gttatccaag gtactgtttt taaaaggtga aataataact aaccggttgc ccggctccgc
     2041 tcaaattccc attcgggaca gatgaaagga gggcaaagat acgaaaatta gtgtgcaagt
     2101 gtgcaagtgt gcatgtgtgc atgtgtgcat gtgtgcaagt gtgcatgtgt gcaagtgtgc
     2161 aagtgtgcat gtgtgcaagt gtgcatgtgt gggattgtgg gagtccggct gagtcgggat
     2221 gtcgctgtgc tctatgtgcg agtgggattt cgcgcgaaga ggctaagaag caaagacgca
     2281 aaaaagaaga cgctaagaag caaagacgca aaaaaactca agctcaccca ctcacccact
     2341 cacccctctc ctctctcctc tctcctctct cctttctcct cccccaatca tttattcacg
     2401 tagatgcgaa caaactcctg ttccggggtc acttttacga gaatgacgta ggttactttg
     2461 gtgaaactag tgtaagaata aacctggtgt ccttctgatt cctggacata ataggtgggc
     2521 atttctttca aaaaatcggt gaaaatcgac ttcccattgg ttcctaccgt gtggaccagc
     2581 tctatggcat cggcattttt tgtgatgaat accatgccat ctgctgtgcc tgttttcgct
     2641 tcaaccgtgt aactgatacc gccatctcca aagtcgtcgc gcagcttgga tatcagtttg
     2701 aggttgcgct cccagtcgcc cagattcatg tccgacatgg ccacgagatt caaaagactt
     2761 acatcgggat aaacgctgcc tgattcagag gtgtaacgcg caacgcgctg ggcgtcaata
     2821 gattgcatgc caataacggc aataaaaagg aggatgaaac ttttcatgtg tgtgtaaact
     2881 aacaatctta tgcaaaacta ataccaaaaa tgaagttgcc gtgcttttag gctttcgggg
     2941 taggatttat tcgcttccaa gcgaaatcca gggccagttg taattgcgct tcaggcgtaa
     3001 ggtctgagtt atcaatcagg atggcgtctt ctgcttgttt caagggactg tcgctgcggg
     3061 tggtttcctc gcgatcgcgt ttttcgaggt ttctgcgaat gtcatccgcc gacaccgttg
     3121 cacctactgc ctgcaattct gccaatcgcc tagccactct tacctccatg cttgcggtaa
     3181 tgaaaagttt gagttcggca tgcggaaaga ctacggtgcc gatatctctg ccgtccatca
     3241 ctaccccacc ctcgcttcct atttgttgtt gttgggccac cagaaacctg cgaatgctgc
     3301 tcactgctgc tacctcactt actttattgc tcaccgccat acttcgtatt tcctcttcca
     3361 ccaacacgcc gttcagccgg gtttgcgtgc gtccgttttg atcggtagcg atatggattt
     3421 tgacttgact aaacacattg ctcagcgcct gttcatcatg ccagttgata tgattgttga
     3481 gcagatatag tgttatcgcc ctgtacatgg ctcctgtatc gaggaaggta tatccgagtt
     3541 tcaatgccaa ttgtcttgcc agggtgcttt ttccgcagcc tgcataaccg tctatggcaa
     3601 tgatgatttt tttcacggag ctaagataag ggttgaccgg tattcacttc atccccgcaa
     3661 gggaaaataa aaaaggttgc cccggtaagg acaacctctt tttggcatgc aagaagttta
     3721 tgattacaaa tgtgtttcct gtgcgttaac aaaggtgtat ttgtcttctg cagaatcata
     3781 tttccaaacg atggcaacca tatacatgtt ttcagatttc catgcgtcgt ttttctcaat
     3841 ttggtatgat ttagtccata caacaccatt agaagcagaa ccgaatacaa actgctcacc
     3901 ccaagcacta ccgttagaag aagcgcgaat tacgtggttg tgaacaaagt taggatcagg
     3961 atcagcaccg gcgatttgtt gcgtgtaaac aagtccgttt tccatgaggt aagcagcaac
     4021 gtagtaatcg ccggttacat cagcaaaaat ttgtgcactt acattgatgg tcatagaaga
     4081 accattatct gtgaaaccgg caacagcacc tgcaacggca ggacctgcac caacttcagc
     4141 agaaacagct cctgccattg gcacaccatc agagaagctt gcctgattcc aaacatataa
     4201 tgtagggaaa ccgccaacaa taccttcagc tgaataatag tcggtaagtg tagtagattc
     4261 gttagtagtt atttcatcag tattgtggat gcccagtgga ataccgttgg cacctgacaa
     4321 attgattgct tcctcaaatt caggtgcacc ccattgtccg caatatggac accaggttgc
     4381 agtgaaatca cccatcaatg cacgcttctg gctggaaggc gcaaagcaac caccatcgtc
     4441 gtcggtagca ttagggttat aattgattga gaaagggtct gtacaaccgt aaacacggtc
     4501 ttttgcacag gaggagaatg ctacagcagc ggttacggct actgtgatga tgagtaattt
     4561 tttcatatgc ctataaaggg ttaggatgaa ttattcctct aagatacatc aaacttctgt
     4621 atatacaagt aattctttcc acatttatga ccaaattctt acgttaattt cgattgacat
     4681 ggggcgaagg aaaggcgctt gtgttacctt taacacttaa aatgacaaaa atgcgcctcc
     4741 tcaagagcct gttaaccctt accggattga tattttcgct atgtgtatat gcccaaccga
     4801 acattgaact cgaaaccttt gcaacgggct tatctaaccc ggtggatatt gcgaatgcag
     4861 gcgacgaccg catgtttgtg gtagaacgca ccggcaaaat ccgcattgtt gaagccgatg
     4921 gcaccgtgca tcccgattat ttccttgata tccataccca gatagaatcg ggttatcagg
     4981 aacagggttt gttgggattg gcatttcatc ccgattatgc tgtcaatgga tatttttatg
     5041 tgtattacac cgatacagat ggtaatacag tggtatcaag gtttagcgta agtgccggtg
     5101 atccggatta tgcagatgcc ggttctgaat tggtattgct cacagaaaat cagccatttc
     5161 tcaatcacaa cggaggctgt ataaaatttg ggggcgatgg ctatttatac atcggattag
     5221 gtgatggcgg ctcagcaggt gatcccggta acagagcgca aaataccgat ctgtttttag
     5281 gaaaaatgct gcgtatcgac gttgatggtg gaactcctta tgccataccg gatgataacc
     5341 ctttcgcgac agcaacagat acgctccctg aaatttgggc tatcggcttg cgtaaccctt
     5401 ggcgattttc ttttgatgca ctcaccaaaa atttatggat aggtgatgta ggtcaaaatt
     5461 tatatgaaga agtagatgtg gaacttgcag gcgacggcgg aagaaattat ggctggcgtt
     5521 gttatgaagg attgcatgaa tttaataccg caggttgcga tgccgatact gctgaatata
     5581 tctttccggt tttagaatat aatcacaatg ctacaacagg tggattcagt ataacaggcg
     5641 gatttgttta tcgcggaagc atgtatcccg gtatgtacgg ttattatatt actgcagatt
     5701 atgtatcggg caactggtgg tggatttatg ccgatggcgg agcaccatat gcaagcttgc
     5761 gtatggatga tattcaaact tccatttctt gttttggcga agacatgaat ggtgaattat
     5821 attgtgcaaa cctgtcgtca ggaattattt atcatatcaa agatgcctgt ggcgatttta
     5881 tggctgctgc tgaagtaaca gattatacct gcggtgttac agatggcgct gttgacatca
     5941 ctatcacagc aggaacagca ccatatacct acgaatggag ctcaggtgaa acaacagaag
     6001 atatttcagg tattaccgaa ggaggcacat ttacattaac tgtaactgat gcctccggat
     6061 gcacgagaat gattactgct actgtggaag aaataccggg atttattgcg gtattaaatg
     6121 aagatggagg tgtacttact gccacacccg gcacttcatg gcaatggtac ttaaatggcg
     6181 aaattattga tggtgccaca tcacaatcat acacaccaac agaatccggt gattatagct
     6241 gcctggtaac cgatgcaaac ggatgcagca gcatgacacc ggcatttaat gtttttgttg
     6301 agataacaac tgctcagtgg aatattgatt tctccataca tccgaatcct gcgagcgatg
     6361 tcatttatct tcaaatcaca gatgcaccat gcgcaacaaa ctggtatatc gccgacatga
     6421 caggtaacgt tgtttggcgt tcggaaacga taggaaaagg aaatcaatcc atcgccattt
     6481 cgctgacctc attagctaac ggcatctatc tcctgcgcag cgatgttggc tcggtgggaa
     6541 gtagggtgat gaaggtgagt ggtgagtggt gagtggtgag ttttgaattc ctctgcgcct
     6601 ctggtctctg cgtgaaaaaa aaaacagatt gtaatgatgt atcaaatcgc gaatatacca
     6661 aagcgcatca cgatacctac tgccattgca ccgttcatac ttttgcgaac agtataagca
     6721 taatcatatc cgcccttatc tattccattg tcataatcac catactgaac gaaggataaa
     6781 ggcattgcaa actgaatacc aatatcagcc ggcaccttgt cgaaaagcac tgtttctccg
     6841 ccaaatacat agcgcaaata actaattcta tgcggatcaa aattcaattg cgacagtgac
     6901 gcaacgggtg tcaattctga agcttgcaag ctaaggttac tgtagtattc ctgatagtca
     6961 acagcataga aatccgtaga actatatcca ctttcttgca ccgaaaattt cccggtcaaa
     7021 atttcgaatt gatgatatct gccaatggga gcgatgtaac catgtttgcg atgactatat
     7081 attttgaaat agaatccggt taacatgccg gatatttttc cttcacccag atatccataa
     7141 ggaatatccg gattagaagc ggtgttataa tacatatgca attgtgcctt cgcctgcttc
     7201 catttcacat cggcacccat tacaaatgtg ttgttcaagg tatactcgat gcccactccg
     7261 ggcgtaacac caattccata attaccatat tcggttggaa tggttacttc gggttgttgt
     7321 aaatagtaat caagtccgtc agtgatgtat ctgaaatcaa acgttggctc aacagatgca
     7381 taaatctgca agcgttttcc catatatccc ggcacctgag caatcgccga tgcggaaatt
     7441 gcgaggctac ataatgctaa aattatcctt ttcatttctt gcgacctttg atttgctgaa
     7501 gaatgtaata aatgttgcta ctctgtgcag cattagaatc attaagtgtg ctgctgttaa
     7561 aatattgcat ctcgagtttc cctgtttccg catttgccac aagcgcaaaa taaaatgtat
     7621 ttctatcagg tgtaacaagg tcataaatca ggaatggtgc caatggaaga tataggcaca
     7681 aaaatatctt agcaccaaca tactcttctg attcggtaaa tgatacgatc cccatccatg
     7741 caaaatgatc aatatcgagg ctttttgcaa tttcctgcaa ttcgtcattt gtggaagtct
     7801 cgataaaaat atcttcatca atatgtgtca gtttctcctc tatccagcga ctaagcactg
     7861 ccatttcgtt gaaggtttca acatctctgt cgtcgatgtt tgtatggtca aggtattcca
     7921 ctttcaagtt taattcgtct gccgatgctt tcactttatc cttaaggttc agcttagcat
     7981 cttcagcggc ttcgtactgc acaggattgt tacttcgttc gtcgatactg atataaacag
     8041 gatctactat caccaattct tttaatccga gattgaactc cgatttcttc ttctttttag
     8101 aagttgttgc aaccaattct tcttcctcct caaacagcgc tttgaattct gccgaatgat
     8161 ctaccgatgc aaatgcaaat cgccagtaag gtgctttttc gtcgatagta tttttttcct
     8221 ttttaatttt atcgtatttg cttgttttgg tatcatttgt tttgccgaca gaatcgacag
     8281 gttgaacggc tgttgagtca tttttcgcat tcatggcatc aataaacttt ttcacctccg
     8341 tttcggtgaa gaaattatca taatcaatgt catgttcatt tctgagttgt ttgccaaggt
     8401 gctgacatat cttggtcatc tgcttattgt caggctccgc gagatgtaca tcccatgcgt
     8461 atttcaatgc cagaatatta aattcttttg catttaattt actgaataag tgaaatactt
     8521 gttgcgactc gccttctacc tttttataat aaatatgacc tgtaggtgtt gttcctgcgt
     8581 tcttgtaaat tgtccaacca tacaatgcct gagcaattac ccttttgaga tagggattat
     8641 ccggaaaatc ttgttgcaat acaaatgcct gatataatgc atcttcataa tgcacatcta
     8701 tcagatataa ttcacatccc tggaagcggg aaatttgttg aatttcatta aatcttgcct
     8761 ctccaaggat atttaatttc cgcgatgcat cacttgttgc aggaatatcg cccaacacat
     8821 ctgatttgcg tttcttgata ttaggatgcg tgcttttagc atcatcgtaa tcttcaactg
     8881 cagtgatttg atttatggta tcaatgaatg cattatcagg gagtttgtac attccttcat
     8941 taaaataatc ttttggaaat acaatttcat taataggcag atagctatac agtagcacat
     9001 caaatacacc tgaaatagca tcataactat agttcgttgt tttgaaaagg tctaagcctt
     9061 tggtatcagc ttcagactcc tgatctttgg cgtaagagaa tttcacaaga tcttcttcat
     9121 cgcgaccaag acttgcataa acaccggcgc cgcgatccac ccttctgttt tcaacgtagg
     9181 aagtaatggc atgtttttcg gtaaaatgca caaattcgtg gcaaagaata tatgccaatt
     9241 gtgcttcatt ttgtacctga gaaaccagtc cggttgtaac tactacaatt ccattatcaa
     9301 aagtgaaagc atttacatcg tttgagagca aggtataaat acgtatagat ttcgccaact
     9361 gcggatcacc ttttaatatt tctgatttaa tgtcattcag ataattggta atcgggtctc
     9421 caaaaacgat acgtccgctg tgcagcatat cttctatttc gtagttggag gcgagcagga
     9481 aattctccat atctttttgc tcgcgtttgc tatcggattc tatttctgat ttatcgcgct
     9541 gaaacttcac ggatgacttt tcagtaaaat catcaggaac agttccggag gatcgcagtg
     9601 tttggaaatt attgaagtcg aaattctgtg ctaccagaat attggtgagc aagagtgtgt
     9661 agatgaggat aatgcttttt atgcgcatgt atgtttactt tattgagatg attcttttaa
     9721 tggtgttttc aggccaattt taatgccggc atgaaataaa gggacgaact gaatggaact
     9781 ggatacatcg ttcaaaataa atacatttga ctgataattt gtaccataac tatttacaat
     9841 cgtattttta aaacgcgttt gaattccaaa tcctacaaaa ccattcattt caaacgtatt
     9901 tctcggcacg ataatatatc ctgccttcca tgacaaactg actgctttca tctgctcact
     9961 gatattgaac aatttgcccg gttcattttc atcctcaaaa tcgaggttgt cgtaccttag
    10021 aaaacggcag gcaaattcca attgattata atcggtttta agcgggtttg gccctcttgg
    10081 aacaagactt aatgcagaaa cttttacgag tggtccatgg taataaaaca cgccactttg
    10141 aaggatatcc tgatagagtt ctgcggtaag cgcaccttct gtatcatcaa catagccaag
    10201 attgaggtct acggcccact cttcaccaat acggtattgc atacccaggt tatactcgcc
    10261 aatcaagaca ttgaagggta tcaggtagac aaatagctgc ttatagagga gcagggaggt
    10321 cataatggac aaatctacca aattggcaga aaaatgtacg gcttttggca gatttcgcgt
    10381 attctggcag tcggaatggg acaggcacaa caattgaaca caggaagcgg ggtggtcgga
    10441 gactgccttg aataaacaaa agatcgcata tgagagatta cggatttgat gatc
//
LOCUS       DAOQHY010000117        10437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_3205_length_10437_cov_8.067617, whole genome shotgun sequence.
ACCESSION   DAOQHY010000117 DAOQHY010000000
VERSION     DAOQHY010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10437
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3205_length_10437_cov_8.067617"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            449..832
                     /locus_tag="PLL28_10105"
     CDS             449..832
                     /locus_tag="PLL28_10105"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNF69719.1"
                     /translation="MMIGELRYKDISEKIIGASFEVHKFLGNGFQEVIYQRALAYEMR
                     KAGLEFAREIEQDIYYKELEVPIGTRRADFVVEGKILVELKAIIQLEDVHLAQALNYL
                     KAYKLEVGLLINFGSKSLTFKRLVL"
     assembly_gap    845..944
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1438..2574
                     /locus_tag="PLL28_10110"
     CDS             1438..2574
                     /locus_tag="PLL28_10110"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNF69720.1"
                     /translation="MSRKKVIVLGAGMVGKAMAIDLAKDYEVYSADINTEALQRLEKH
                     GVRTIHADLTSKELIQSLIEDKDLVIGAVPGFMGFETLKTVIESGKNASDISFFDEDP
                     FLLDALAKQNGVTAVMDVGVAPGMDNIILGYHNKRMQVTDFVCLVGGLPAARLWPYEY
                     KAPFSPIDVIEEYTRPARFVQDGKVVVKEALTDPEYMDFTGIGTLEAFNSDGLRSLIK
                     TMPHIPNMIERTLRYPGHIALMKVLRETGFFNKEFIDVKGTHVRPIDVTSKLLFPKWK
                     LGDEEHEFTVMRVIVGGIENDNQVTYTYDLLDRYDPATKTSSMARTTGYTCTAAARLI
                     LEEMYTRKGISTPEFLGEEEAHFHFIMRHLGERGISYQKSQKLR"
     gene            complement(2631..3365)
                     /locus_tag="PLL28_10115"
     CDS             complement(2631..3365)
                     /locus_tag="PLL28_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69721.1"
                     /translation="MRNLLIFGTTMVLMHCALHAQDSSRIVINTININDYTIELVKVQ
                     QIDEVAVKRLNYQTPYSWLYQIYEKDSFIIAVQYFKDSCELYFDSRIKKQGFYYTTHG
                     VKINICDKTVTQIMPPDVSWIKDTFDSITIYPLDSVLDPYDYQGQRTVISNYSEREPE
                     HLNLQHQKDVEFMYRDPRVIGMYQKGSELRLHSALTYYKSGNAQTIYLYYDKFMYEGW
                     FYNVGYSEHGTYNVAKMVWDKHEAAK"
     gene            complement(3728..5014)
                     /locus_tag="PLL28_10120"
     CDS             complement(3728..5014)
                     /locus_tag="PLL28_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69722.1"
                     /translation="MFIQKIVYLDNVFQINNAGVLKPVSGTSTNIQPLIIQHFQLREE
                     GNQHKKQLPLFNPSDFDYYGLYVPESFAYFISPSIGNNPSPDQRMIIIAGADSMVIDF
                     HNIPKTTRYELTILQQIYFMPGYYSLDCSLPARDLKINENEREILYAAVKHGITPETK
                     SILSKAGLLEMALPTDIVKYQKYIPAAITVEQSDAYNINVYFTGNLLTTSNGILITDF
                     QESYRGHWHSTAFSTTFHYIGEESGTSAVFYENQKRVISQTNPMGERNLTDGWYRIGV
                     VNSYGDSIYSKPFCVGLVAANSDSVTTLYNKQRGFPFHNFKDANTINIETAVAPVIID
                     HQPKYVFKYHIVVCNLCLFSDELYGRYDQLAAEEFMHRLAFNDLYINGQPFTGSIEIQ
                     FPIKGATVENGGAWFYTNLIVTNTYINGKLQKHSER"
     gene            complement(5275..5850)
                     /locus_tag="PLL28_10125"
     CDS             complement(5275..5850)
                     /locus_tag="PLL28_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HNF69723.1"
                     /translation="MTKGRLEAFSDGVIAIIITIMVLELKVPHSAEWSALHELIAVFM
                     SYVLSFVYVAVYWGNHHHLLHTVKNVSAGIIWSNAALLFFLSLIPFATAWMGENHFAQ
                     NTVIAYSILMNLCGMSYYVLMYMIMQCHSENAELTAALNTQRRKGILSLFLYSFSIGA
                     AFINPMISQVIFVLVAIMWFIPDKNIKRLMR"
     gene            complement(5853..6704)
                     /locus_tag="PLL28_10130"
     CDS             complement(5853..6704)
                     /locus_tag="PLL28_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HNF69724.1"
                     /translation="MLQTILFDFQAFFGHSPTEAAIIVFNLILIESMLSVDNALVMGT
                     MVMDLPKEQRGKALRYGIFGAYFFRGLALLLATQLIKVVWLKALGGLYLLYLALDYFR
                     SSATNDISDDTLDKKSNWFYKNTVGRIGNFWSTVILVNIMDLAFSIDNVFAAVAYTDN
                     IYLICTGVFIGILAIRFVAQGVIKLLERFSFLNTSAFLIIGLLGLKLLASFICNEYGR
                     ETPFCLFLESEKTDLYFSLITVCIFILPVITSWLFNFPKRSHDHHTDEFAHFQEEADE
                     AEHPKHS"
     gene            complement(6715..7740)
                     /gene="galE"
                     /locus_tag="PLL28_10135"
     CDS             complement(6715..7740)
                     /gene="galE"
                     /locus_tag="PLL28_10135"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNF69725.1"
                     /translation="MNTILITGGCGYIGSHTAIDLLEKGFNVISIDNLSRSKTFIADR
                     IREISGKQFHNYRVDCRDLVKMRNIIDQHGKIDGIIHFAAFKSVGESVVKPLVYYENN
                     INSTLNMLRIADEFHIPNFVFSSSCSVYGNTAQQPVKEDAPMATPESPYGWTKLISEQ
                     MIAAHAPNVQTKFIALRYFNPVGAHQGGRIGEIPYSEPENLVPNITQTAIGKKKGFTI
                     FGTDYPTRDGSCIRDYVHVMDIAAAHTAALQYQLSGQQQVPFDVFNLGSGRGVTVLEL
                     IRAFERSTGVSLPFEIGPRRQGDVVAIYANTEKAMQSLHWHPTRSIEEMMHSAWTWEQ
                     TMQRESI"
     gene            complement(8141..9001)
                     /gene="rfbA"
                     /locus_tag="PLL28_10140"
     CDS             complement(8141..9001)
                     /gene="rfbA"
                     /locus_tag="PLL28_10140"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNF69726.1"
                     /translation="MKGIILAGGSGTRLHPITLAISKQLMPIYDKPMIYYPLSTLMMA
                     GIKDILIISTPYDLPGFQRLLGDGSRIGCSFSYVEQPSPDGLAQAFTLGADFIGNDKA
                     ALVLGDNIFYGSGLGELLTANNNPDGGIIYAYHVSDPERYGVVDFDAAGNVLSIEEKP
                     LQPKSNYAVPGLYFYDNTVVDIARNLKPSPRGEYEITDVNKEYLRQGKLKVSVLPRGT
                     AWFDTGTFDSLMAASTFIQLIEQRQGQNIGCIEEIAYRKKFINAEQLEKIAQPLMKSG
                     YGQYLMRILK"
     gene            9215..10348
                     /locus_tag="PLL28_10145"
     CDS             9215..10348
                     /locus_tag="PLL28_10145"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNF69727.1"
                     /translation="MLSNIIIKPLTHRGGEHYALFFSYDKKLVDQAKKAGCRFSFQNK
                     CWYLPATKENLHKLFTVFKGVAWLDTKAIKHNSGIAKPVEKKNRVSVAEPLPDARLAE
                     LEKLKEWMQHKHYSERTIKVYLDCVRLYFSVNRDKDPHAINREDFISFIREHIIARKL
                     SFTYQNQFVNALKLFLQMVYGLHVNTDFLERPRREHKLPNVLSKQEVLSILKHTDNIK
                     HNLMLRLAYGCGLRVGEVLALRVDDIDRMRRCIQIRLAKGFKDRVVPLPISVEPILNM
                     YIERYQPKYYLFEGEKPNSKYTERSLQLVLKKSVTLSGITKPVTMHWLRHSYATHMLE
                     SGVDTRFIQVLLGHKNIKTTEIYTHVSTHQINAISSPIDSLNL"
BASE COUNT         3252 a         1999 c         2001 g         3085 t
ORIGIN      
        1 aattagaggt ggatttctaa tgaaaattat ttttattgag aagacatgca aaaaattgca
       61 cattaacatc atttgaataa ttcattagtg cgcgaaggtc tgaatgctta gaccgctccc
      121 ctataaattc ttaaatccgt gaaatccatt aatccgtcca atccgtgatt cagacatctt
      181 taaaagcaat ggccggcttc taagtatctc acttgaaaac cggagcgaaa aaacactcct
      241 gcttgcgctc aagatcacat gcgcatatga acacacgaac aaaataaata atgtgcaatt
      301 attgtctgaa tcacggatta caaggattta gagattacac agatttaaag attagacgga
      361 ttttttaaga ctaattgtat gtatacttgc agatttatat tgaatatgaa attatttagt
      421 aaataaatta tatttaaatt ttatttgcat gatgattgga gaattgaggt ataaggacat
      481 ttcggagaaa atcattggtg cttcgtttga agtgcataaa ttcttgggca acggctttca
      541 agaggtcatt taccaaaggg ctttagcgta tgaaatgcgc aaagcgggat tggaatttgc
      601 cagagagatt gaacaggata tttattacaa ggaattggaa gttcccatag gcacacgcag
      661 ggctgatttt gtggttgagg gcaaaatctt ggttgaactc aaagcaatta ttcagctaga
      721 agatgtgcat ctagctcaag ccctaaacta ccttaaggcc tacaaactag aagtaggact
      781 actcatcaac ttcggcagta aaagtttaac ctttaagcgc ttagtgttat gaatagaaca
      841 attgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaatccg tgattcagac
      961 atcttcggca gtaaaagttt aacctttaag cgcttagtgt tatgaataga acaattgaat
     1021 atcaaatacc gaaaacctca ctctacaaca atcgaaacaa atccatgtaa tgcattaatc
     1081 cgtgaaatcc attaatccgt ctaatccgtg attcagacat cttcggcagt aaaagtttaa
     1141 cctttaagcg cttagtgcta tgattgacac tagtctatct catttttcat caaccatact
     1201 caacaaacgc tctcatagat ttccaaatcc atgaaatcct aaatccgcga aatcctttaa
     1261 tccgtctaat ccgtgattca gacaacttca gccgtattgt gcaactttca cacaagaaca
     1321 gaaatgcagt gtgtaagtgt gaaagtatgt aagtgtgctg cataatacac gatataaacc
     1381 ccatcgcccc acaaagcaat ttacgtttta acctatcttt acggcaaata atttgctatg
     1441 tcaagaaaaa aagtgatcgt acttggtgcc ggaatggtgg ggaaagctat ggctattgat
     1501 ctggccaagg attatgaagt gtattctgcc gatatcaata ccgaagcgct tcaacgcctt
     1561 gaaaagcatg gtgtgcgcac cattcatgcg gatctgacca gcaaggaatt gatacaatca
     1621 cttattgaag ataaggacct tgtgattggg gctgtgccgg gttttatggg atttgagaca
     1681 ttgaaaactg taattgaatc gggtaaaaat gccagcgata tatctttttt tgatgaagat
     1741 ccttttttac ttgatgcatt agcgaaacaa aatggggtta ctgcggtaat ggatgttggt
     1801 gtggcgccgg gaatggataa tatcattctg ggttatcaca acaaacgcat gcaggtgaca
     1861 gattttgttt gtttggttgg tggcttacct gctgctagat tatggccgta tgaatacaaa
     1921 gcaccgtttt caccaattga tgtgatcgaa gaatatacac gtcctgccag atttgttcag
     1981 gatggaaaag ttgttgtcaa agaagctctt actgatcctg aatatatgga tttcacaggc
     2041 atcggcacac tggaagcatt taattcagat ggcttgcgct cacttatcaa aaccatgcct
     2101 catattccaa atatgataga acgcacgttg cgttatcccg gtcatattgc attgatgaaa
     2161 gtattgcgcg aaacaggatt tttcaacaaa gagtttattg atgtgaaagg cactcatgtt
     2221 cgccctattg atgtaacatc aaaattatta ttccccaaat ggaagctagg cgatgaagaa
     2281 catgaattta cggtaatgcg tgtgatcgtt ggcggaattg aaaatgataa ccaggttact
     2341 tatacctatg atctgctcga cagatatgat cctgctacta aaacttcttc catggcgcgc
     2401 acaacgggat atacatgcac tgcggctgcg cgattaatat tggaagagat gtatacaaga
     2461 aaaggaattt ctacgccgga gtttttagga gaggaagaag cacatttcca ctttatcatg
     2521 cgccatctgg gtgaacgtgg aatcagctat cagaagtcgc aaaagcttcg ctaataggat
     2581 acaacaaaat gtaaatatga tacggaaata catattttcc gtatgtatta ttatttcgcc
     2641 gcctcgtgct tatcccacac cattttcgct acattatatg ttccatgttc ggaataaccg
     2701 acgttgtaaa accagccttc gtacataaac ttatcataat ataaatatat tgtctgtgcg
     2761 tttccggatt tataatacgt taatgcggaa tgtaatctta actctgagcc tttctgatac
     2821 atccctataa ctcttggatc ccgatacata aattccacat ctttttgatg ttgaaggttt
     2881 aaatgctcag gctctctttc ggaataattt gaaattactg ttcgttgacc ttgataatcg
     2941 taaggatcta gcactgaatc gagcggatat atggtaattg agtcaaaagt gtctttaatc
     3001 caactgacgt caggaggcat gatttgcgta actgtcttat cacaaatatt aatctttacg
     3061 ccatgtgtcg tataataaaa tccttgcttt tttattcggg aatcaaaata aagttcacat
     3121 gaatctttaa agtattgaac cgcaattatg aatgaatcct tttcataaat ttgatataac
     3181 caagaatagg gagtttgata attaagcctt ttaactgcaa cttcgtctat ttgttgaacc
     3241 ttgaccaatt ctatggtata atcattgata ttaattgtgt taattactat ccgactggaa
     3301 tcctgtgcat ggagagcgca gtgcattaaa accatagtag tgccgaatat caataggtta
     3361 cgcataaaat ataaatctag ttttcaaagt taagatgcaa gttcaatata aattccataa
     3421 taaatcataa aatattttga gaagagccga aaataagggc caattaacaa tttggccaag
     3481 gataaggatc aatatacggc tctagtttag ggttaataat aagaataaaa atgcattgca
     3541 aagcattatt ttcgtaattt acctgtttgc cggaaaaaaa cattttgcag gtatctcatt
     3601 gttaaactaa aaggataaac acgctagctt aaataaatac ttagccatgc aaaacccaac
     3661 actgcataac gtacactcat tttcgtctaa cgcatatatg ctcagaactt cctactcaga
     3721 tcccacctca tcgctccgaa tgtttttgta attttccatt tatataggtg tttgtaacaa
     3781 ttaaattagt ataaaaccat gcacctccgt tttctacagt agccccttta atcgggaatt
     3841 gtatttcaat gctgccggta aatggttgtc cattaatata caaatcgttg aatgctaatc
     3901 tgtgcataaa ctcttctgcg gcgagttgat catatcggcc atacaactca tcagaaaaga
     3961 gacataggtt gcatactaca atgtgatact tgaacacata tttgggctga tggtcaatta
     4021 ttacaggcgc aacagcagtt tcgatgttga tggtatttgc atctttgaaa ttatgaaatg
     4081 gaaaaccacg ttgcttatta tataatgttg ttacactatc gctatttgcc gcaactaatc
     4141 ctacacagaa gggtttagaa taaatcgaat ctccatagct attgacaacc cctattctat
     4201 accatccgtc tgttaaattg cgttcaccca ttgggtttgt ttgagatatt actctctttt
     4261 gattttcata aaataccgca gaggtgccgg attcttcgcc aatgtaatga aacgttgtgc
     4321 taaatgctgt agaatgccaa tgcccccggt aggattcttg aaagtcagta ataaggatac
     4381 cattacttgt tgtaagcaaa tttcctgtaa aataaacatt tatattatag gcatcagatt
     4441 gctcaacggt tatagcggcc ggaatatatt tctgatattt tacaatgtct gttggtagtg
     4501 ccatttcaag caaccctgct ttactaagta ttgattttgt ttccggtgta attccatgtt
     4561 ttacagcagc ataaagtatt tcccgttcat tttcatttat tttaagatct cttgccggta
     4621 aagaacaatc caaactataa tatcccggca taaaataaat ttgttgcaaa atagttaatt
     4681 cataacgtgt cgtttttgga atattatgaa agtcgatgac catagaatct gctcctgcta
     4741 taatgatcat acgttggtca ggagacggat tatttcctat gctaggagaa ataaaataag
     4801 caaaagactc gggaacatac aatccataat aatcaaaatc ggatggatta aacaatggaa
     4861 gttgtttttt atgttgattc ccttcttccc ttaattggaa atgctgaatg attagtggct
     4921 gaatattagt tgatgtccct gaaaccggct taagaacacc tgcattattt atttgaaaca
     4981 cattatccaa ataaacaatt ttttgaataa acaagccccc atccttttct tgaggttgtg
     5041 ctgaaagggt aaagaagcca caagtgagca gaaatgtata taacaaagac ttcatatgac
     5101 caaaggacag catctactac caattattat gccagctaaa ccaatggact aaaatatgat
     5161 atatttttat actaaaaagt ggaattttta atatgcatta atcaaatgaa gcatccatct
     5221 tatttcaata aatacattat ggatggccta atttagcaga aaatatagtg cagattatcg
     5281 catcaagcgt ttaatattct tgtcggggat aaaccacata attgcaacca aaacaaaaat
     5341 cacctggcta atcattgggt tgataaatgc cgctccgatt gagaatgagt aaagaaataa
     5401 actcagtatt ccttttctgc gctgcgtatt caaggccgct gttaattcag cattttcgct
     5461 gtggcattgc atgatcatat acatcaatac ataatagctc attccacata aattcatcaa
     5521 tatggagtat gcgatcactg tattttgtgc aaaatgattt tcacccatcc atgctgtggc
     5581 gaatggtatc agcgacaaga aaaataaaag tgccgcatta gaccagatga taccggcaga
     5641 aacatttttt acagtatgca acagatgatg gtgattgccc caatacactg ccacataaac
     5701 aaaactcaac acatagctca taaataccgc aatcagttca tgcagtgcgc tccactctgc
     5761 gctgtgcggc actttcaatt ccaacaccat aatggttata atgatggcga ttacaccatc
     5821 gctaaatgct tcgagtctac ctttggtcat agttatgaat gcttggggtg ttcggcctca
     5881 tcggcttctt cctgaaagtg cgcaaactca tctgtatgat gatcatgact tcgcttcgga
     5941 aagttaaaaa gccaacttgt aataaccggc aaaataaaaa tacaaactgt aatcaatgaa
     6001 aaataaaggt ctgttttttc actttcaaga aataaacaga atggggtttc tctgccatat
     6061 tcattacaaa taaatgaagc taataatttc agccctagca atccaatgat gagaaatgcg
     6121 gaggtattca gaaagctaaa tcgctccagt aattttatta ccccttgtgc aacaaaacgt
     6181 atggcaagta tgcctataaa aacacccgta cagatcaaat aaatattgtc tgtataggca
     6241 acggcagcaa acacattatc gatggagaat gccaggtcca tgatatttac cagaataacc
     6301 gtactccaga agttaccgat tcttccgaca gtatttttat aaaaccaatt tgatttcttg
     6361 tcaagcgtat cgtccgaaat atcattcgtt gcgctgcttc gaaaatagtc cagagcaagg
     6421 tatagcagat ataatccgcc cagtgccttc agccaaacaa ccttaattaa ttgagtagca
     6481 agcaataatg ccaggcctct aaaaaagtat gcgccaaaaa tgccatatcg caaagctttg
     6541 ccacgctgtt cctttgggag gtccattacc attgttccca tcaccagcgc attgtctaca
     6601 ctgagcatgc tttcaatcaa gataagattg aacacgatga tagccgcctc agttggcgag
     6661 tgtccgaaga atgcctggaa atcgaataaa atggtttgca gcatggtttg taatttaaat
     6721 agattccctc tgcattgttt gctcccacgt ccaggcagaa tgcatcattt cctcaataga
     6781 gcgggtagga tgccaatgaa gtgattgcat tgctttttcg gtatttgcat aaattgcaac
     6841 tacatcaccc tgacggcgcg gaccaatttc aaatggcaat gaaacccctg tactacgttc
     6901 aaatgcgcga ataagttcaa gcacggtaac gcctcgtccc gagcccaagt taaatacgtc
     6961 gaaaggaact tgttgctgtc cggaaagttg atattgcaat gcagcggtat gtgctgcggc
     7021 aatatccatc acgtgtacat aatcgcgaat gcagcttccg tcgcgggtag gataatcggt
     7081 tccgaaaatg gtaaatcctt ttttcttacc aattgctgtt tgggtgatat tcggcaccag
     7141 attttcaggt tcactgtaag gaatttcgcc aatccttcca ccctgatgcg cgcccaccgg
     7201 attaaaatat cgaagcgcaa taaattttgt ttgcacattt ggagcatgtg cagcaatcat
     7261 ttgttcgctg atgagtttag tccacccgta aggcgattcg ggtgtggcca ttggtgcgtc
     7321 ttctttaacc ggttgttgcg cggtatttcc ataaacactg caagatgaag aaaatacgaa
     7381 attgggaata tgaaattcat cagcaatacg caacatattg agtgtgctgt tgatattatt
     7441 ttcataatat accaatggct taacaacaga ttcgcctacc gatttaaatg cagcgaaatg
     7501 aatgatgccg tcaatttttc catgctgatc aataatattt cgcattttca ccagatcacg
     7561 gcaatcgacg cgataattgt gaaattgctt cccgctgatt tctcgaattc tatcggcaat
     7621 aaaagtcttt gagcgcgaaa gattatctat agaaataacg ttaaacccct tttccaaaag
     7681 gtcaatagcc gtatgagagc caatgtatcc gcaaccgccg gtgatgagaa tagtattcaa
     7741 tagaatttgt ttaattagct gattagcaaa ttagctaatt agctgatgga atttttttaa
     7801 ttagccaatt agcaaattag ctaattagct gatggaattt tttaattagc caattagctg
     7861 attagccaat tagccgatgg aattttttta attagccaat tagccgatgg aattcaatta
     7921 ttttctgttg tactgattta ctaatttatt tgtttgtttg ccaactttca aacctactaa
     7981 cttgctaacc tgcaaacttg caaacttgct aacttgccaa ctttcaaacc tgctaacttg
     8041 ctaacttgct aacttgccaa ctttcaaacc tgctaacttg ctaacctgct aacttgccaa
     8101 ctttcaaatt tgctaatttg ctaatcagct aatttgctaa ttacttcaag attctcatta
     8161 aatattgccc atagccactt ttcattaacg gttgtgctat tttttcaagt tgttcggcgt
     8221 taataaattt cttgcgatag gcaatttcct caatacatcc gatattttgt ccttgtcgtt
     8281 gttcaattaa ttggatgaaa gtactggcag ccatcaatga gtcgaaggtg cctgtatcga
     8341 accatgcagt gcctcttggc agcacactta ccttcaattt tccttgacgg agatattctt
     8401 tatttacatc agtaatttca tattcgccac gcggactagg tttaaggttt cttgcgatat
     8461 caaccacggt attatcgtag aaatacaatc cgggaacagc ataatttgat ttaggctgaa
     8521 gtggtttttc ttcaatggac agcacattgc ctgctgcatc gaaatcaaca acgccataac
     8581 gttcggggtc gctaacatgg taggcataaa taattccgcc atcggggttg ttgttagcag
     8641 taaggagctc gcccagtccg ctgccataaa agatattatc accaagcacc agggcagcct
     8701 tatcgttccc tataaaatcg gctccgaggg taaatgcctg tgcaagccca tcgggggaag
     8761 gttgttctac ataagaaaat gagcatccta ttcgagatcc gtcgcccagc aatctttgga
     8821 agcccggcag gtcgtaaggg gtgctgatga tcagaatatc cttgatgccc gccatcatga
     8881 gggtcgacag ggggtagtag atcatgggct tatcgtatat gggcataagc tgtttgctga
     8941 tagccaaagt aattggatga agccttgtgc cggatccgcc ggctagaatg attcccttca
     9001 ctttaagtgg tttgcggcaa agatagggtc agaagaggag tttattgagt ttttagggca
     9061 tcaaaggttg attgattgca cgggatttta gcagaaaata ccttttccaa cacaaaacaa
     9121 gtcaatgaaa ccggcgggca gttagtcaat acacagcaac gttcaaggca gaacggagat
     9181 tttggggtaa cttctatggg taactaaagt tttcatgcta tcaaatatta tcatcaaacc
     9241 gcttacgcat cgtggtggag aacattacgc attatttttt tcttatgata aaaaacttgt
     9301 tgaccaggca aaaaaagcgg gttgccggtt cagcttccaa aataaatgtt ggtatttgcc
     9361 tgccacaaaa gaaaatctgc ataagttatt cacagtattt aaaggcgttg cgtggttaga
     9421 tacaaaagca ataaagcaca atagtggtat agcaaaaccg gttgaaaaaa agaaccgggt
     9481 ttcggttgca gaaccattac ctgatgcgcg cttagcggaa ctggaaaaat taaaggagtg
     9541 gatgcaacac aagcattata gcgaacgcac aataaaagtg tatcttgatt gtgtgcgatt
     9601 atatttctcg gttaacagag acaaggatcc gcatgccatt aaccgggagg attttatttc
     9661 attcattagg gagcatatca ttgctcgcaa attatcgttc acatatcaga atcagtttgt
     9721 aaatgcgctg aaactatttt tgcaaatggt atatggtttg catgtaaaca ctgattttct
     9781 ggagcgtccg cgacgggaac acaagcttcc aaacgtattg tcgaaacaag aggtattatc
     9841 aatattaaaa cacactgata acatcaaaca caatctgatg ttacgtttgg cttatgggtg
     9901 cggattgcgt gttggggaag ttttggccct gcgggttgat gatattgacc gcatgagaag
     9961 gtgtatacag atccgactgg caaaagggtt taaggatcgg gttgtgcctt tacccatatc
    10021 tgtcgaacct atattaaata tgtatataga gaggtaccaa ccaaaatact atttatttga
    10081 aggggaaaag cccaacagta agtataccga aaggagtctt caattggtat taaaaaagtc
    10141 agtaacttta agtgggatca ccaaacctgt taccatgcac tggctgcgac attcatacgc
    10201 cacgcatatg ctggaatcgg gggttgatac gcgatttata caagttttac tgggacataa
    10261 gaacatcaaa accacagaaa tatacaccca tgtcagcaca catcagatca acgccatcag
    10321 cagtccgatc gacagcctca acctctaata taagccgaaa ccattttaac atttccttcg
    10381 gctatactcc ggtatagata ttaggaagga aataatgctt cggcaataaa caagtta
//
LOCUS       DAOQHY010000118        10406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_3224_length_10406_cov_6.130809, whole genome shotgun sequence.
ACCESSION   DAOQHY010000118 DAOQHY010000000
VERSION     DAOQHY010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10406
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3224_length_10406_cov_6.130809"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PLL28_10150"
     CDS             <1..256
                     /locus_tag="PLL28_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69728.1"
                     /translation="TVGDGELAAPTKGGVGRGLASWQMWECLHVYLCGSATLREKSEN
                     LYWDNNYLAIYKFERWQVSNCDWVRAKWRRRTVNHTGART"
     gene            complement(328..1458)
                     /locus_tag="PLL28_10155"
     CDS             complement(328..1458)
                     /locus_tag="PLL28_10155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HNF69729.1"
                     /translation="MALLFNENEANCTLGVEMELQVLDAERLLLIPRGYEIIQAVNSP
                     RLAKEMFRSTLEIVTGICNNVHDVHHDFLESLEQVKSYAEKNNLRFAGTGTHPLAMYQ
                     DRITTTGKRYQDLMDRNQWLIRRMAVYGLHVHIGMHNAEECMRFNHFFLRFVPHLIAL
                     SASSPYWQCSDTGLAAARPTMYEAHPTSGIPYLATDWSDFERVYHAMIDTGSIESMKD
                     IWWDIRPSPTYGTLELRMCDGPATLLELESIVALIHVLALWFNDHGDRYFKEHRAIPD
                     RWVLRENKWRVIRNGIDAVLIDPDTLHTEKFVSAMSYWLEQVQPYAEKFGYENYMQTI
                     KQILTKGNSAQRQRDVFKNTGSLEKVVAHNVTEFEQGKPQWL"
     gene            complement(1863..>2047)
                     /locus_tag="PLL28_10160"
     CDS             complement(1863..>2047)
                     /locus_tag="PLL28_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69730.1"
                     /translation="IRDSDNIWAVFICSTWNKEEKGERKEEWVSREFLCGLCNLAYLL
                     EPTSLPPSPIVDREGS"
     assembly_gap    2048..2147
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2586..4448
                     /gene="mnmG"
                     /locus_tag="PLL28_10165"
     CDS             2586..4448
                     /gene="mnmG"
                     /locus_tag="PLL28_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNF69731.1"
                     /translation="MFKEYDVIVVGAGHAGCEAAAAAANMGSSVLLVTMNMQTIAQMS
                     CNPAMGGVAKGQIVREIDALGGYSGIVTDKTAIQFRMLNRSKGPAMWSPRAQSDRMLF
                     ANEWRLMLERTPNVDFWQDMVSGLLVKDGRVCGVRTGMGIEIPSKAVVLTNGTFLNGL
                     IHIGEKQFGGGRAAEKAATGITEQLVELGFESGRMKTGTPPRVDGRSLDYSLMEEQPG
                     DEDPQKFSYTDTKPLAKQRPCHITYTSDAVHDILRTGFEKSPMFSGRIKGPGPRYCPS
                     IEDKIERFSSRERHQIFVEPEGWDTVEVYINGFSTSLPEDVQYKALKAIKGFEHVKMF
                     RPGYAIEYDYFPPVQLNHTLETRLVKNLFFAGQINGTTGYEEAACQGLIAGINAHLAV
                     KEDAPFVVDRSEGYIGVLVDDLINKGTEEPYRMFTSRAEYRVLLRQDNADIRLTPRGH
                     AIGLASDARLERVRQKEQASADLIKFLQQTSIDPDQINGRLEMLGSAAIRQKTKLALL
                     LSRPHIEMQHLLGLNEALDATILSFEPEFREQAEIIIKYEGYISKEKELVEKMQRLEH
                     IVIHDTIDYSLIHSLSSEAREKMQRIRPKTLGQASRISGVSPSDIAVLMVHIGR"
     gene            4456..4821
                     /locus_tag="PLL28_10170"
     CDS             4456..4821
                     /locus_tag="PLL28_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69732.1"
                     /translation="MQIEKLIPVLWTEHVEESIHFYTEILPFQLSAHDREANWANVVW
                     GEIEIMFSTPNAHVEQRAIGFTGSFYFRCTDVETLYHAISTKVKLIYPLEVFSYGMKE
                     FGCYDNNGYILNFGEQIDQ"
     gene            4818..6491
                     /locus_tag="PLL28_10175"
     CDS             4818..6491
                     /locus_tag="PLL28_10175"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HNF69733.1"
                     /translation="MIRAANFKASDTISFRYIYIYVLLMYGCANVVTPTGGEKDVLPP
                     VALSFSPDTNSLFFNTNEIKITFDEFIQLSDAFNQVLISPPLNNTPDYKVKGKTLTIK
                     LNDTLKENTTYTINFGQSIKDITESNILNNFTYVFSTGEQLDSLQVKGRSVDMLSGKP
                     SEKAYAVLYYEPTDTSFTTSRPYYFARIDKEGNFTIKNIRSGKYRLYVLEDMNFNYYY
                     DLPNERIAFQDSILEVNGEIPLQKMSVFSEDKLPQSLTDIKSPRYGMTRMVFAKNASN
                     VSIKYTGTDTAETYIARNVTGDTLTFWHSNYALDSHKLAIQFDTTLLDRTIAIKTFPK
                     DSDFTKQHNTFTTNAIPINKGAGARADWDPERDIILKCYNPVTQLSTSPKVYSDSILL
                     GDATWFTDTLDATKVYIRYPFKPETNYDIRIEAGSMHDIFGLTNLSDTIFIKTRKLDA
                     YGTLNIALTNSSNSQLIVELIKFDLSPVQNWVLPVEQFQNETHTFNIKKSYLLPGVYR
                     IRVTKDTNGDGKWTTGELVQYRQPETIIYYPTDQNVRANWDNEIEWEIK"
     gene            complement(6685..6930)
                     /locus_tag="PLL28_10180"
     CDS             complement(6685..6930)
                     /locus_tag="PLL28_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69734.1"
                     /translation="MQHLHPIPRFINSKARTMTMLEYAKMILQKVSFDQGLFRTELYK
                     AIRKLMQEEVSELKKWCLETFGVSYCMEAAPQLLIEG"
     gene            7052..>8475
                     /locus_tag="PLL28_10185"
     CDS             7052..>8475
                     /locus_tag="PLL28_10185"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HNF69735.1"
                     /translation="MKHFAASILVILLGTFVAKAQITAFPDTSICPGDLVTLGSVTVD
                     ICADCYSYEEIPFAPETIGGESITMVDDTYIGPYDIGFDFCFFGVYYDQFYLCSNGWI
                     SFVPPGAGWATNWTPDGPIPDAASNVPKAAIFAPWTDWHTGLCTDCIHYEVFGVTPNR
                     RIAITWEEVPLFSCTDYLGTFQIVLYETSNWIDNHFMDVDICPTWDLGIATQGLQNED
                     ATAAYTVTDRNATAWSATDESWRWYTSILEWYDEDGVLVGTGPEVDVSPEFTTTYTLV
                     QTLCDGTSYTDDVTVTVGADLTIDSDITAVSCGGAGDGSISISVDGGIEPYTYEWSTG
                     ATGVTELSGLDGGTYTVTVTETGGCSRTYTFDVIEPEELVAEATDIVNNPCNGYDEGS
                     VHIDVTGGSIPYSYSLDGGASTILSDFTGLEAGDYTVTVTDASGCTTTAEFTITEPIL
                     LTADLTADDTEIFVGQGSTITLTT"
     assembly_gap    8476..8575
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9081..9239
                     /gene="rpmH"
                     /locus_tag="PLL28_10190"
     CDS             9081..9239
                     /gene="rpmH"
                     /locus_tag="PLL28_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNF69736.1"
                     /translation="MKRTFQPSKRKRKNKHGFRERMKTKDGRKVLARRRRKGRKKLTV
                     SDERNISK"
     gene            9274..9669
                     /gene="rnpA"
                     /locus_tag="PLL28_10195"
     CDS             9274..9669
                     /gene="rnpA"
                     /locus_tag="PLL28_10195"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNF69737.1"
                     /translation="MLRKSERLCQQQLIASLFTAGHRFLCRPFQLVVLTTPLNSPEPA
                     QLLISVSRKRFPRAVDRNRIKRHLREAYRHHKGELYTYLQQHNMQVAFAVVYTGKSVT
                     TFNEMNDRIPVALSLIVKQLETLRSKSEG"
     gene            9669..9887
                     /gene="yidD"
                     /locus_tag="PLL28_10200"
     CDS             9669..9887
                     /gene="yidD"
                     /locus_tag="PLL28_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNF69738.1"
                     /translation="MNIFSYIIIIPVKLYQWVISPILPNSCRYTPTCSHYMLDAVKKY
                     GPIKGGWMGLKRLSRCHPWGGSGYDPVK"
     gene            9899..>10406
                     /locus_tag="PLL28_10205"
     CDS             9899..>10406
                     /locus_tag="PLL28_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNF69739.1"
                     /translation="MQSRNKMKLTNRFIAGLIGVATIFIGTSAATSADLYEVGKNIDI
                     FTSIYKELNTYYVDDVDPNKLMRTGIDAMLESLDPYTNYISEAEIEGYKFQITGNYGG
                     IGASIRTIEGKSTVMECYPGFAADKAGIIPGDIITEIDGKKLNGDNNESVSDLLRGSP
                     GSTVNIKIY"
BASE COUNT         3005 a         2087 c         2292 g         2822 t
ORIGIN      
        1 gacggtagga gatggagagc ttgccgctcc gacgaaagga ggtgtggggc gggggttagc
       61 aagttggcaa atgtgggaat gcctgcatgt ttatctctgc ggctctgcga ctctgcgtga
      121 aaaaagcgag aatttatatt gggataataa ctacctcgca atctacaagt ttgagcgttg
      181 gcaagttagc aactgtgatt gggttcgcgc aaagtggcga aggcgcacag tcaaccacac
      241 cggcgcccgc acctgaacac ccgaacacga tcacccgaac acaaaagaat ttcagcattt
      301 aaagaagaca actaacttca tatcaaccta cagccattgt ggttttccct gttcaaattc
      361 cgtgacattg tgggcgacta ctttttccag tgatccggtg tttttgaaaa cgtcgcgttg
      421 acgttgtgcg ctgtttcctt tggtgagaat ttgttttatt gtttgcatat aattttcgta
      481 tccgaatttt tcggcatagg gttgtacttg ttcgagccaa taagacattg ctgaaacaaa
      541 tttttctgtg tgcaaggtat ccggatctat cagcacggcg tcaatgccat ttcttattac
      601 acgccattta ttttcgcgaa gtacccatct gtcgggaatt gctctgtgct ctttaaaata
      661 tctatcgccg tgatcgttaa accataatgc taatacatgg atgagcgcaa caatactttc
      721 caactctaat aatgttgcag gaccatcgca cattcttaat tcgagtgtgc catatgttgg
      781 acttggtcga atatcccacc aaatatcttt catactttca atcgaaccgg tatctatcat
      841 tgcatgatat acgcgttcaa aatcactcca atcggtagcg agatacggaa taccacttgt
      901 aggatgtgct tcatacatgg ttggtcgcgc agcagctaat ccggtatctg aacattgcca
      961 atatggtgat gaggcgctta gtgcaatcag atgtggaaca aatcgaagaa agaaatgatt
     1021 aaatcgcatg cattcttccg cattgtgcat gccgatatgt acatgtaaac catatactgc
     1081 catgcgacgt attaaccatt gattgcgatc cattaaatcc tgatatcttt ttccggtagt
     1141 ggtaatccta tcctgataca tggcaagcgg atgtgtgccc gttcctgcga atcgcaaatt
     1201 atttttttct gcatacgatt ttacttgttc aagcgactcc aaaaaatcat gatgcacgtc
     1261 atgcacatta ttacaaatac ctgtgacaat ttcgagggtg ctgcgaaaca tttcttttgc
     1321 cagtcgcggc gagtttactg cctgaataat ttcgtaacct cgaggtatta gtaatagtcg
     1381 ctcggcatcc agcacttgca attccatttc tacgccgagg gtgcagtttg cttcattttc
     1441 attaaatagt aaggccattc ttcttcattt tatcgttaac aactacaatt atcaaaatcc
     1501 aaggtgcgca aaattccggg gcgcgatttc ggcaatgcta tatgcaaata gcataagaac
     1561 cgataatcag attgtgctaa tttaataaac gaaatgattg gaggcaattt ttggggatgg
     1621 gattaacata gtttagcaca tttttcgttc gcaaataatt gattttcaat gagatgtgat
     1681 ttcgtgggcg aatttaagca ggcgggctaa ttagcaagtt tgagggttag caagttagca
     1741 agtgtggggg ttagcaagtt agcaagtgtg ggggttagca agtgtgattg gcctgttaac
     1801 gccggcgcgt taacagggaa tgcaattata ttgctttgca atattttaat tccgaagtga
     1861 aattagctcc cctctctatc gacgatagga gatgggggga gtgaggtggg ttcgagcaaa
     1921 tacgccaagt tgcaaagccc gcaaagaaat tctctactca cccactcctc tttcctctct
     1981 cctttttcct ctttgttcca cgtggaacat atgaatactg cccatatatt gtctgaatca
     2041 cggattannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgt tccacgtgga
     2161 acatatgaat actgcccata tattgtctga atcacggatt acggggatta aaggattgca
     2221 cggatttgtg ggctattctg accattgttt caccctccgg tcaatatcac actcacccac
     2281 ccgcctgccc ggcgaggtaa actcacccac tcaccctctc ctttttcctc tttcctttct
     2341 cctcattgtt ccacgtggaa catatgaatg ctgcccatat attgtctgaa tcacggatta
     2401 aggggattaa aggattgcgc ggatttgggg agcattctaa ccattaaatc accctccggt
     2461 taaaatctca cccgcctgcc cggcgaggca agctcaccca cccgcctgcc cggcgaggta
     2521 aactcaccca ctcctctttc ctctctcctt tttcctctct cctttctcct tatctttgcc
     2581 ctctcatgtt caaagaatac gatgtcatag tggtgggtgc cggccatgcg ggctgtgagg
     2641 cggcagcggc ggcggctaat atggggtcta gtgtgctgtt ggtgaccatg aatatgcaga
     2701 cgattgccca gatgagctgt aaccctgcaa tgggtggggt ggcgaagggg caaattgtcc
     2761 gggagattga tgccttgggt gggtattcgg gaattgtgac cgataaaacc gctattcagt
     2821 ttagaatgct caacaggtcg aagggacccg cgatgtggag tccacgagcg cagagcgaca
     2881 ggatgttgtt tgcgaacgaa tggcgactca tgctggaaag gactccgaat gtcgatttct
     2941 ggcaggatat ggtgtcggga ttgttggtga aggatggaag ggtatgtggt gtgagaacgg
     3001 gaatggggat tgaaatacca tcaaaggcgg tggtgctgac caatggaacc ttcctaaacg
     3061 gactgattca cataggcgag aaacaatttg gtggtggcag ggctgcagag aaagcggcta
     3121 ccggaattac agagcagctg gtggaactgg gatttgaaag cggcagaatg aagaccggaa
     3181 caccgccaag ggtggatggc aggagccttg attatagcct catggaggaa cagcccggcg
     3241 atgaggatcc gcagaagttc tcgtatacag atacaaaacc cctggccaag cagaggcctt
     3301 gtcatattac ctatacatca gacgcggtgc atgatattct gagaacaggg tttgagaaat
     3361 cgccgatgtt ctcgggaagg atcaagggtc cgggaccaag gtactgtcct agtattgaag
     3421 ataagattga aaggttctct tccagagagc gtcatcagat atttgttgaa ccggagggat
     3481 gggataccgt ggaggtatat atcaatggtt ttagtaccag tctgcccgag gatgtgcagt
     3541 ataaggcgct aaaagctatc aaaggatttg agcatgtgaa gatgttcaga ccggggtatg
     3601 ctatagaata tgactatttc ccgcctgtcc agttgaatca tacattggaa accaggttgg
     3661 tgaaaaatct cttttttgca gggcagatca atggcacaac cggttacgaa gaggctgcat
     3721 gtcaaggact tatagcagga atcaatgcac atctggcggt aaaagaagat gccccgtttg
     3781 tagttgatcg cagtgaagga tatatcggtg tactggtaga tgatcttatt aataagggga
     3841 cagaagagcc gtatcgcatg ttcacctcca gggcagaata tcgcgtgttg ctgcgtcagg
     3901 acaatgccga tatcaggctt acgcctcgcg gacacgctat aggtttggca tctgatgcgc
     3961 ggttagagag ggtgagacag aaagaacaag caagtgccga tttgataaaa tttcttcaac
     4021 agacaagcat agatccggat caaataaacg gcagacttga aatgttgggc agtgccgcca
     4081 tacgtcaaaa aacaaaactc gcgttattgc tcagcagacc gcatatagaa atgcagcatt
     4141 tgttagggtt gaatgaggcc ctggatgcta ccattctcag ctttgaaccc gagtttcgcg
     4201 agcaggcgga gatcattatt aagtatgaag gctatatcag caaggaaaaa gaactggtag
     4261 aaaagatgca gcgactggaa catattgtca ttcatgatac cattgattac agcctaattc
     4321 attcactctc ttctgaagca agagaaaaaa tgcaacgcat cagaccgaaa acccttggtc
     4381 aggccagcag gataagtggt gtttcaccat ccgatatcgc cgtgctgatg gtgcatattg
     4441 gcagataatt cacacatgca aatagaaaaa ttaattccgg tgttgtggac tgagcatgtt
     4501 gaagagagca ttcacttcta cacagagatc ttaccatttc aattatcagc tcacgatcgc
     4561 gaagcaaatt gggcgaatgt ggtttggggt gaaatcgaaa ttatgttcag cacacctaat
     4621 gcgcatgtcg aacaaagagc catcggtttt accggtagct tttatttccg atgtacagat
     4681 gttgaaacat tatatcatgc tatttcaact aaggtgaagt tgatttatcc tctggaagta
     4741 ttttcatacg gcatgaaaga attcgggtgc tatgataata acggatatat actcaacttt
     4801 ggcgaacaga tagatcaatg atccgcgcag caaatttcaa agcatctgat acaatttcat
     4861 ttcgatacat atatatatat gtattgctga tgtacggatg tgcaaacgtg gtgacaccca
     4921 ccggcggaga aaaagatgta ctacctcctg ttgcgctttc attttctccc gatacgaatt
     4981 cattattctt caacaccaat gaaataaaaa taacctttga tgaattcatt caattaagtg
     5041 atgcatttaa tcaggtattg atttcacctc cattaaataa tacacccgat tataaggtaa
     5101 aaggaaaaac actcaccatt aaattaaatg atacgcttaa agaaaacacc acttatacca
     5161 tcaacttcgg gcaatcaata aaagatatta ccgaatctaa tatattaaat aatttcacat
     5221 atgtattttc aaccggcgaa caattggatt cgttacaagt aaaaggaagg tcggttgata
     5281 tgcttagtgg taagccgtcg gaaaaagctt atgccgtatt atactacgaa ccaacagata
     5341 catcatttac cacatcgcgt ccttattatt ttgcccgcat agacaaagaa ggaaatttca
     5401 cgatcaaaaa tatccgctca ggaaagtata gattatatgt gttggaggat atgaacttca
     5461 attattatta tgatctgccg aatgaaagaa tagcatttca ggattccatt ttagaggtga
     5521 atggcgaaat tcctctacag aaaatgtcgg tgttttctga agacaaattg ccccagagtt
     5581 taaccgacat taaatcgccc cgctacggca tgacgcgtat ggtatttgca aaaaatgcct
     5641 cgaatgtgtc gattaaatat acaggaacag ataccgctga aacctatatc gcacgcaatg
     5701 ttacgggtga cacgcttacc ttctggcata gcaattacgc gcttgattca cataaacttg
     5761 cgatacaatt tgacacaaca cttttagata gaaccattgc aatcaaaaca tttccaaaag
     5821 attctgattt caccaaacag cacaatacat tcaccacaaa tgcaattcct ataaataaag
     5881 gagcgggtgc aagggcagat tgggatccgg aacgcgatat aattttgaag tgctataacc
     5941 ccgtaacgca actatctact tcaccaaaag tatatagcga tagtattttg ttaggcgatg
     6001 cgacatggtt tactgataca ttggatgcta caaaagtata tatccgctat ccgttcaaac
     6061 ctgaaacaaa ttacgatatc cgaatagagg caggaagcat gcatgacatt ttcggcttga
     6121 ctaatctttc agataccatt ttcattaaaa caagaaagct cgatgcttat ggtacattaa
     6181 atattgcact gactaattcg agcaattcgc aattgatagt cgagttgatc aaatttgatt
     6241 tatcgccggt acaaaattgg gtgttacccg tggaacaatt tcaaaacgaa acccacactt
     6301 tcaacataaa gaaatcgtat ttacttccgg gtgtctatcg catacgcgta acgaaggata
     6361 ccaatggcga tggaaaatgg acgacgggag aattagtaca atatcgacaa ccggaaacaa
     6421 taatatatta tcccaccgat caaaatgttc gcgcgaattg ggataatgaa attgagtggg
     6481 agataaaatg atggcaatta ttcgtacgat ttgaaattga tttttctcct tcgcacatat
     6541 caaatgctaa cttcaaaaat ttcaaacagc cttaaaaagt cgatctcaca gctgcgcgct
     6601 gttaaaagaa aaagaaattt agatggcaga acatcaaaaa cattctgcgt tgcttaaatc
     6661 gaaaataaac ggcaaataat caaatcaacc ctcaatcaag agttgagggg cggcttccat
     6721 gcagtaggaa acgccaaatg tttcgagaca ccattttttc agttcgctga cttcttcctg
     6781 catcaatttt ctgatggctt tatataattc ggtacgaaaa agtccttgat caaaactcac
     6841 cttctgaagg atcattttgg cgtattcaag catcgtcatt gtcctggcct ttgagttaat
     6901 aaaacgaggt attggatgca aatgttgcat tttttacagc tttaacaatt ggcaattttt
     6961 cacagatttc gaacgaaatc ctactaaata agacactgcg tttttggctt tccggcaaat
     7021 cattatcttt atcgttcata ttcatcgtgt tatgaagcat tttgcagcct ccattctggt
     7081 tatcctcctg ggcactttcg ttgctaaagc acaaatcaca gcatttcccg acaccagtat
     7141 ttgtccgggc gacctggtta cgctaggatc ggtaacggtg gatatttgtg ccgattgcta
     7201 tagttatgaa gaaatacctt tcgccccgga aacaatcggc ggtgagtcga tcacgatggt
     7261 agatgacacc tatatcggac catatgatat cggtttcgat ttttgcttct tcggtgtgta
     7321 ctatgatcaa ttctacctct gctctaacgg atggataagc tttgttccac cgggagccgg
     7381 ctgggctact aactggacgc ctgatggccc tattcctgat gcagcttcca acgttccgaa
     7441 ggcggcaatc tttgcgccgt ggaccgactg gcatacgggc ttgtgtaccg attgtattca
     7501 ctatgaagtt tttggcgtta ccccaaaccg tcgcattgct attacctggg aagaagtgcc
     7561 gctgttcagc tgtacagatt acctcggcac attccagatt gtattatatg aaacttcaaa
     7621 ttggatagat aaccatttca tggatgtaga catttgtccg acttgggatt tgggtattgc
     7681 aacacagggt ttacaaaatg aagatgcaac agcagcttat accgtaactg accgcaatgc
     7741 tacagcctgg tcggcaactg atgaatcctg gcgctggtat accagtattt tggaatggta
     7801 tgatgaagat ggcgtattag taggaacagg tccggaagtt gatgtttcac ccgaattcac
     7861 aacaacatac accttggtgc aaacgctttg cgatggtaca tcttataccg atgatgtaac
     7921 cgttaccgtt ggtgccgatt tgaccataga tagtgatatc actgccgtaa gctgcggtgg
     7981 cgcaggagat ggctccatca gtatttctgt tgacggcgga attgaacctt atacctatga
     8041 atggagcacc ggcgcaacag gcgttaccga attgtcgggc ctggatggcg gcacgtatac
     8101 tgttactgtt acagaaacag gtggatgctc gcgcacttat acttttgatg taattgaacc
     8161 cgaagaattg gttgccgaag ctaccgatat tgtcaacaac ccttgcaatg gttatgatga
     8221 agggtcagta catatagatg ttaccggagg tagtatacct tattcctata gtctggacgg
     8281 cggcgcctcc accatattga gtgatttcac cgggttggaa gcaggagatt atacagtaac
     8341 ggtaacggat gcaagcggtt gcacaactac tgccgaattc actatcacag aaccaatact
     8401 attaactgca gatctaactg cagatgatac cgaaatattt gtcggacaag gctctaccat
     8461 tacactgacc accagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngaagt
     8581 catgttcgaa accaatgata tagcgggtcc gggctggaat ggcgttaccg atggtgagcc
     8641 tcagggcatg ggcacttatg tttggcaggt tgatgccggc tttatcaatg gtgaaaagtt
     8701 ctctgaaaca ggaaacttcg tactagtaag ataattattg caacaaccac tattcgattt
     8761 aacccaaaac cactcttttt cggggtgcaa ctgttttact ccaaaaccat tactgtttaa
     8821 tgctttgcgg gtaacatatc aacacaaatt ttaatatatt acatattatg tttttattat
     8881 atgcattatc gcatagacca agtatagtaa tagttttagg cgaaatgaaa tatcagaatt
     8941 catttacctg cttaaaagaa gctaaaccct aaacagaaac cacggttttc agtgttcgaa
     9001 attgttggca attaattttg tggacagatt gtgaatttcg gtgaggtaat gttatctttg
     9061 cgttcccaaa ttaattagtg atgaaaagga cgtttcagcc ctcaaaaagg aaaaggaaga
     9121 ataaacacgg ttttcgcgaa cgcatgaaaa ccaaagacgg ccgtaaagta ttggcacgtc
     9181 gccgtcgtaa aggacgcaaa aaactgaccg tttctgacga aaggaatatc agcaaataat
     9241 attggttccg gtagcgctaa gttgccggac gccatgctgc gcaagtctga acggctgtgt
     9301 cagcaacaac ttattgcctc gctatttacg gcggggcatc gttttttatg ccgacccttt
     9361 caattggtgg tgttaaccac accattaaat tccccggaac ccgcacagtt actcatttcc
     9421 gtatcgcgca aacgctttcc ccgcgccgtt gatcgcaacc gaatcaaacg tcatttacga
     9481 gaagcatatc gtcatcataa aggcgaatta tatacctatt tgcaacagca caatatgcaa
     9541 gtagcatttg ccgtcgttta tacaggaaag tctgtaacaa cttttaacga gatgaatgac
     9601 aggatacccg tagctttatc gttgatagta aaacaattag aaacacttcg atcgaaatca
     9661 gaagggtgat gaatattttt tcttacatca taattattcc cgtaaaattg tatcaatggg
     9721 tgatatcacc aatacttccc aatagttgtc ggtatacccc aacatgcagt cactacatgc
     9781 ttgatgccgt aaaaaaatac ggaccaatta aaggtggctg gatgggatta aaaagattat
     9841 cgcgttgcca tccctggggt ggaagtggct atgatccggt gaaataattt atgaattaat
     9901 gcagagcaga aacaaaatga aattaacaaa tagatttatt gcaggactaa taggtgtggc
     9961 gacaattttc atcggcacca gtgcagcaac gagcgcagat ctttatgagg taggaaagaa
    10021 catcgacata tttacttcta tctacaaaga attaaatacc tattatgttg atgatgttga
    10081 tccgaataaa ttgatgcgca cgggaattga tgcgatgttg gagagtttgg atccatatac
    10141 gaattatatt tctgaagctg agattgaagg atataaattt caaataacag gaaattatgg
    10201 cggcattggt gcgagtataa gaacgataga aggaaaatct accgtgatgg aatgttatcc
    10261 gggattcgct gctgataagg cgggaattat tccaggtgat atcattactg aaattgatgg
    10321 taagaaatta aatggcgata acaacgaatc ggtaagtgat ctgttgcgcg gatcaccggg
    10381 gtcaacggtt aatattaaaa tatatc
//
LOCUS       DAOQHY010000119        10175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_3327_length_10175_cov_5.077668, whole genome shotgun sequence.
ACCESSION   DAOQHY010000119 DAOQHY010000000
VERSION     DAOQHY010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10175
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3327_length_10175_cov_5.077668"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="PLL28_10210"
     CDS             complement(<1..865)
                     /locus_tag="PLL28_10210"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HNF69740.1"
                     /translation="MPFYHKMGQVPQKRHTQYRQPDGSLYHEELFSTEGFSNTYSLLY
                     HLYPPTKIKQVGEAYSVEPKVVLSRQLKHRSLKGFKIAPVDDFLESRKAVLVNSDLHI
                     SLAAPKKSMTDYFYKNVDADEMLFIHEGTGKLHTAYGEIAFEYGDYIIIPRGTLYQIS
                     FDTEQNRLLIVESFSPIEFPKRYLNKVGQLMEHAPFCERDIKTPVNLQTYDQQGDFYF
                     KIKKQGIIYPYHYANHPFDLIGWDGYVYPWAFSIHNFEPITGRVHQPPPVHQTFEARN
                     FVVCSFVPRMYD"
     gene            complement(977..2155)
                     /locus_tag="PLL28_10215"
     CDS             complement(977..2155)
                     /locus_tag="PLL28_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HNF69741.1"
                     /translation="MEPAPRVDFNDTATAFSGRTDKALKWEYQLFRLMNNPAATNVLT
                     SLASLSIKMHLPVKWAIKKTVYEQFCGGETLEETKLVLSELERFGIGAIIDYGVEGKE
                     SEAEFERTKNELIHIIQFAAGKKNIPFISCKVTGLTAFATLEKLNSRVELTTAEQKEK
                     DALYGRMFAIAGAAADAGIGLFVDAEESWIQDAIDDLTFELMRRFNKQKAVIFSTPQM
                     YRHDRLAFSKRSLEDAVQHGYILGLKPVRGAYMDKERKRAQELGYQDPIQPDKAATDR
                     DYDAVIQFSLDHIDKIALCAATHNENSSLLLANALNDRGIAHNHPHIWFSQLFGMSDQ
                     ITYNLAKSGYNVAKYLPYGPVKDVIPYLIRRAQENKSVSGQMSRELSLIHKEIKRRGI
                     "
     gene            2203..3546
                     /locus_tag="PLL28_10220"
     CDS             2203..3546
                     /locus_tag="PLL28_10220"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69742.1"
                     /translation="MTNVYLYKIVLGGVRKHLVCLSLIVSGCLGAKAQNYISYFTGDT
                     TDVETIPTAGTVLMGGATENDNAMRWFLERAGGGDVVVLRTTGDDGYNDYFYSDLGVI
                     VNSVETIICNDAASGADPYVIHQVENAEALWFAGGDQWDYIQYWRNTPLEDVFNVLIN
                     DKKIPIGGTSAGCAIQGEAYFSAENGTITSTQALHDPYHNLMTLGYSDFIDNPGTKQI
                     ITDTHYDNPDRRGRHIAFLARLYQETGVPYYGIGVEEYTAVCIDENNIAHVFGSYPYY
                     DDYAYFLQVNCVEPNTPETCVDGSLLTWDRSSAAVKVIKMAGMDFGEGSVDLNDWKTA
                     VGADFQWEDWSVDNAIFSALEMTSPINCDTLLTVENNILKNVVIYPNPATDHISLQNT
                     GSDFDYRILNISGDCIFKGTMNSNDQINVRDIPAGLYVIEIRSDNYLDYQSFLKH"
     gene            complement(3557..4738)
                     /locus_tag="PLL28_10225"
     CDS             complement(3557..4738)
                     /locus_tag="PLL28_10225"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNF69743.1"
                     /translation="MIWLGICLHTQAFYAQSFISNGDFETMTLCPDTTSMLVLAEGWF
                     EANLANPDYFNACDIEGVVGVPENFIGSESAHSGSGYAGIYVAENDGPVYREFLESAL
                     TGSLMPNHAYAVSLYLSVADISSCFPTSLCIYLSSEPMLEPLTDGYLTHYELLQQMLC
                     LEPGNIIDDMDGWYHAYFCFKATGDEHFITIGNNYSEAQAPCINITGGAQKAYVYIDD
                     VSLTEMPVTSVEMDTFICKGSAISIDALDHIEQPANSEFSIVWNDGGTGSKRTFTEEG
                     IYTAEIDNGCSTDTFRITLHWRMDCPEIFYFPNAFSPNNDGLNDRYYPQIENITIAQI
                     DIYDRWGALVYSGIQDPNGWDGTVNGKDAEIGLYMYVIRYTTNISEKSVEKYGALQLI
                     R"
     gene            4893..6674
                     /locus_tag="PLL28_10230"
     CDS             4893..6674
                     /locus_tag="PLL28_10230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNF69744.1"
                     /translation="MQSFVVSARKYRPASWHDVIGQDQVVTTLKNAIRTDHLAQSFLF
                     CGPRGVGKTTCARILAKTINCENITKEGEACNECESCKSFNENASFNIHEIDAASNNS
                     VEDIRSLVDQVRYAPASGKFKIYIIDEVHMLSPQAFNAFLKTLEEPPTYVKFILATTE
                     KHKIIPTILSRCQIFDFNRIRIRDMVKQLEIICKSEGITCTEEALHLVAQRADGAMRD
                     ALSIFDRITSFSGKSIEAKDVIDNLNILDYEYFFRITECAVSEDATTALVLFDEIINK
                     GFDGDVFINGLAEHFRNLLMCKEEKTIGLLDTSEAAIEKYTSQSQMIPASFLLSGLNL
                     LNSADIQYRGAKNKRLHVELVIMKLCYIHNAVSALASGSVEKKTPDLNKTVQLKPKIE
                     PQREAEPLPVNPSFRPEPKSEVPPVQKVVVQNNAINPPVPEQIETVMIPKKTDLRQSV
                     RKEEEQRKLEVPVVQVTQEKILIEPEDFFVAWKQFAAFIRAEKQAEAIIMDHAAVSID
                     DRNIVTVKLDSSYEVNILKGVHNELHDFIKQSLQNNTFEINLVVEAAKQEKVKRPYTA
                     QEKYKKMEELNPLIRDLKEKLGFELDY"
     gene            6703..9822
                     /locus_tag="PLL28_10235"
     CDS             6703..9822
                     /locus_tag="PLL28_10235"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNF69745.1"
                     /translation="MKLHRIICLGLGAIVLSITSLSAQTGSSSSSSSGNQGQSGSSSQ
                     SGYIPNSSSSSASQGGAPGPIRIEPNTPDAITIFQTTNASPGVNTAKATKYDFITVPD
                     DPTRTRIYTLKNGLKVYLSQNKAEPRIQTYIAVRTGSNNDPKETTGLAHYLEHMMFKG
                     TTSFGTNNWDREKMVIEGISQLYEKHRRETDPEKKKAIYAEIDKLSQEAAKFAIPNEY
                     DKMITSLGAKGTNAYTSKEQTVYVNDIPANELEKWLVIESERFRTCVLRLFHTELEAV
                     YEEYNMGQDRDGSKVYETYYRALFPTTPYGTQTTIGEGEHLKNPSMVNIMGYFYTYYV
                     PNNMAVCLSGDLDYDKTIALIDKYFGAYVNKQVGVREFNTEKPITEVQRREVYGQESA
                     YVQLGYRVPGANTEDNIIAKLIAGMLYNQQAGLLDLDLNQQQKVLDAAAYHTEWRDYS
                     VLTLAADPRDGQTLEECEQLLQAELEKIRKGEFDEWLMKAVINDYQLSRTKSFESNSG
                     RAGAFVNTFIRGIDWGAYLNEYNVMSTITKADIVEFANTYLGKNSYVVVYKRKGEDKN
                     IKKVEKPVITPIEPNRDMSSKFLTSFMELPSSRVAPEFVDYSTKISNTMLNNDVPMAY
                     IKNEENQLFELYYILDMGSYNDRELALAINYLPYLGTDKYSPAQLQQEFFKYGLSFTV
                     NAGSDRVYVSLSGLESNLEKGIELFEHLLSHVQPDNKAYREYVDGIIKEREDAKTNKN
                     YIFGVAMLNYAKYGAVSPQTDILSEQQLRNVLPATLTDKIKSLTGYKHRVFYYGQMRP
                     ELVQTMLNKYHVTKAPLMDYPPATKYPEKETNENKVLFVHYPMVQAQISFISKDENFD
                     KTVLPQARMFNEYFGSGLSSIVFQEIRETKALAYSAYANYSTPLNKDEAHYVRASVST
                     QADKMQAAMEAMLEIMNNMPQAEEQFNASLESVLKQIESERIIRSNIFWNYESMKRKG
                     LNYDYRRDVYMAARGMSMQDMQEFFNNHISDRHYTIIVMGDRTKVDLNYLKSIGAFRE
                     MTMEEIFGY"
     gene            10016..>10175
                     /locus_tag="PLL28_10240"
     CDS             10016..>10175
                     /locus_tag="PLL28_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69746.1"
                     /translation="MPRMSDTMTEGTFVEWHKKVGDTVKIGDILAEVETDKATMELEN
                     YAKGTLLYI"
BASE COUNT         3115 a         2044 c         2146 g         2870 t
ORIGIN      
        1 aatcatacat gcgcggaaca aaactgcaaa caacaaaatt tcttgcttca aaagtttgat
       61 gaaccggagg tggctgatgt acgcgtcctg ttattggttc aaaattgtga atagaaaagg
      121 cccaaggata cacatatcca tcccatccga tcaaatcaaa aggatggttg gcataatgat
      181 agggataaat aattccctgc ttttttattt taaaataaaa atcgccctgt tgatcatacg
      241 tttgcaaatt gacaggtgtc ttgatgtcgc gctcacaaaa tggcgcatgc tccattaatt
      301 gaccaacttt gttaaggtac cgcttcggaa attcgatagg cgaaaaactt tcaactatta
      361 ataatctgtt ttgctcagta tcgaaactta tctgatagag tgttccgcga gggataatta
      421 tgtaatcgcc atactcaaat gcaatttcgc catatgcagt atgtaatttg ccggttccct
      481 catgtatgaa aagcatttca tcggcatcta cattcttgta aaaataatca gtcatactct
      541 ttttaggagc ggcgagtgaa atatgtaaat cgctatttac taacacggcc tttctgctct
      601 ctaaaaaatc gtcaacaggt gcaattttaa accctttcaa agagcgatgc tttaattgac
      661 gactcagcac cacttttggc tcgacactgt atgcctcacc tacctgcttt atcttggttg
      721 gaggatatag atgatacagt aatgaataag tattgctgaa gccttcggtt gaaaacaact
      781 cctcatgata taagcttccg tcaggctgtc gatactgggt atggcgcttt tgaggcacct
      841 gtcccatttt atggtaaaat ggcatggtat tcctgttaag tatgctccgt aaaattacaa
      901 aataatcatc ccgcgcgcgg tgacttttgt caacctacgg tgggttctga caattatctg
      961 agaaagtata ttagacttaa atacctctgc gtttgatttc cttatgaatc agggaaagtt
     1021 ccctgctcat ttgaccgctt acggatttgt tttcctgggc tcttctaatc aaatatggga
     1081 ttacatcctt gacaggacca taaggcaggt atttcgctac attgtagcct gattttgcca
     1141 ggttgtaagt gatctggtcg ctcataccaa acaactgcga aaaccaaata tgtggatgat
     1201 tgtgcgctat tccacggtcg tttagggcat ttgccaatag caaactgctg ttttcattgt
     1261 gtgtggcagc acataaggcg attttatcaa tatgatcgag cgaaaattga ataacggcgt
     1321 cgtaatcgcg gtcagtggca gccttatcgg gttgaatagg atcctgataa cccagttcct
     1381 gagccctttt gcgctccttg tccatatacg cgcctcgaac aggtttcaaa cccagaatat
     1441 aaccatgttg aacggcgtct tccaatgacc gcttgctaaa tgcaagtctg tcgtggcgat
     1501 acatctgcgg agtgctgaaa ataacggctt tttgcttgtt aaatcgacgc atcaattcaa
     1561 aggtcaggtc atctatggca tcttgaatcc agctttcttc cgcatctacg aatagtccaa
     1621 taccggcatc ggcagcagca ccggcaatgg caaacatgcg tccgtaaagg gcatcttttt
     1681 ccttctgctc tgcagttgtc aactcgaccc tcgaattcaa cttctcaagg gtagcaaatg
     1741 cagtcagacc ggtaaccttg caactgatga atggaatgtt tttcttgcct gctgcaaatt
     1801 ggatgatatg gataagctca ttcttggtgc gctcaaattc cgcttcagac tcctttcctt
     1861 caacaccata atcaatgatt gctcctatgc caaaacgctc taattcgctg agcacgagct
     1921 tggtttcttc taaggtctct cctccgcaaa attgctcata aacggtcttt ttgatggccc
     1981 attttaccgg caaatgcatt tttatcgata atgatgccag ggaggtgagt acattcgtag
     2041 cagcaggatt gttcatgagc ctgaacaact gatattccca ttttaatgcc ttgtctgtcc
     2101 ggcctgaaaa tgcagtagcc gtatcgttaa agtcaacacg cggtgcgggt tccaaaatat
     2161 gatgaatttg tgcaaatata cgtggttgta tatttttatg ggatgactaa tgtttattta
     2221 tataaaatcg tcttaggggg tgtaagaaaa cacctcgtat gtcttagtct catcgtttcc
     2281 ggttgccttg gagcgaaagc gcaaaactat atttcctatt ttaccggtga tactactgat
     2341 gtagaaacaa taccaactgc aggtacagtg ctgatgggtg gagcaacaga aaacgataat
     2401 gcaatgcgat ggttcctcga acgtgccggt ggtggcgatg tggttgtgtt gcgcactacc
     2461 ggtgatgatg gctataatga ttatttctat tcagatcttg gggttattgt taactctgtt
     2521 gaaactatta tatgcaacga tgcagcttca ggcgccgatc catatgtaat ccatcaagta
     2581 gaaaatgcag aagcattatg gttcgcaggc ggtgatcaat gggattatat tcaatattgg
     2641 aggaatacac cattggaaga tgtatttaat gtccttatca acgataaaaa aatcccaatc
     2701 ggcggaacaa gtgccggttg tgccattcaa ggagaagcat atttttccgc agaaaatggg
     2761 accattacat caacacaagc attacacgat ccataccata atttaatgac attaggatat
     2821 agcgatttta tcgacaaccc tggcacaaaa caaattataa cggatacaca ttacgacaac
     2881 ccggacagac gcggtcgtca tatcgctttt cttgcgcgat tatatcaaga aactggtgtg
     2941 ccatattatg gtatcggcgt tgaagaatat accgctgttt gtattgatga aaacaatatt
     3001 gcacatgtgt ttggcagtta tccctattat gatgattatg catatttctt gcaagtaaat
     3061 tgtgtggaac cgaatacacc ggaaacttgt gtagatggtt ctttgctcac atgggacaga
     3121 agtagtgctg ctgtgaaagt tataaaaatg gcgggaatgg attttgggga aggaagtgtt
     3181 gatctgaacg actggaaaac tgcagtggga gcggattttc agtgggagga ctggtcggtt
     3241 gacaatgcga tattcagtgc attggaaatg acatcgccga taaattgcga tacgttgttg
     3301 acggtggaaa ataatatcct gaaaaatgtg gtaatatatc cgaatcctgc tacagaccat
     3361 atatccttac aaaataccgg cagcgatttt gattatcgta ttcttaatat ttccggagat
     3421 tgcatattta aaggaactat gaattcaaat gatcagatta atgttagaga catacctgcg
     3481 ggactttatg taattgaaat acgaagtgat aattatttag attatcaatc ttttctgaag
     3541 cactaaattc cgggattcat cttattaatt gtaaagcacc gtatttttct accgattttt
     3601 ctgagatatt ggtggtgtat ctgattacat acatataaag accaatttct gcatcctttc
     3661 cgtttacggt tccatcccat ccgtttggat cctgaatgcc tgaatatacc aatgctcccc
     3721 atctgtcgta aatatctatt tgggcaatag taatgttctc gatttgcgga taatagcgat
     3781 catttaatcc atcattattt ggagaaaatg catttgggaa ataaaatatc tccggacagt
     3841 ccatccgcca atgtaatgta atacgaaaag tatctgttga acacccgttg tcaatctcgg
     3901 ccgtatatat gccttcctca gtaaatgtgc gtttactgcc tgtgccacca tcgttccaaa
     3961 caattgaaaa ctcactattg gcaggttgtt caatatggtc tagtgcatca atgcttattg
     4021 cagatccttt gcaaataaag gtatccattt caacgctcgt aaccggcatt tcagtcagtg
     4081 aaacatcgtc aatatataca taggctttct gtgcccctcc ggtaatattt atacatggag
     4141 cttgtgcttc ggaatagtta tttccaatgg taataaaatg ttcgtcgcct gtagccttga
     4201 aacaaaaata ggcatgatac cagccatcca tgtcatcaat gatatttccg ggttcaagac
     4261 ataacatttg ctgcaatagc tcgtaatgtg taagatatcc atcagttaat ggttccagca
     4321 ttggttctga agacaaatag atacacaatg atgtcgggaa acatgaactg atatcggcaa
     4381 cacttaaata taaacttacc gcataggcgt gattgggcat cagcgaacct gttaatgctg
     4441 attccaaaaa ttcgcgatac acaggtccat cattttcagc tacataaatg cctgcatatc
     4501 cgcttcctga atgcgctgat tcactgccaa tgaaattttc aggaacacca actaccccct
     4561 caatgtcgca ggcattgaaa taatctggat tagccagatt ggcctcaaac catccctcag
     4621 ccaataccag catagaagta gtatccgggc aaagggtcat ggtctcgaaa tcgccattgc
     4681 tgataaaact ttgtgcataa aatgcttgtg tgtgcaaaca aatgccaagc cagatcatca
     4741 aaccaatccg ggaaagttta acaatatagc tggttacgtt gggcaattag ggttaataga
     4801 tttcattaca aatttaggtt ttgggcatgg tacttgtgta atctgatggt aaaatcaggg
     4861 atacgacacg ctaaattgta tatctttacc ccgtgcagag ttttgttgtt tcagccagaa
     4921 aatacaggcc ggcgtcatgg catgatgtga ttggacagga tcaggtagta actaccctga
     4981 aaaatgccat tcgcaccgat caccttgccc agtcgttcct gttctgtggt ccgcgtggtg
     5041 tgggtaaaac cacctgtgcc cgtatcctgg cgaaaactat caattgcgaa aatattacca
     5101 aagaaggtga agcctgcaac gaatgcgaaa gttgtaaatc ttttaatgaa aatgcatctt
     5161 tcaatattca tgaaatcgat gcggcttcta acaactccgt agaagatatt cgaagtctgg
     5221 tcgatcaggt gcgatatgcc cctgcaagcg ggaaatttaa gatatacatt atcgacgagg
     5281 tgcatatgct cagccctcag gcattcaatg cttttttgaa gacattagag gagcctccta
     5341 cctatgtaaa atttattctc gctaccactg aaaagcacaa gatcattcct accatcctca
     5401 gcagatgtca aatctttgac ttcaatcgta ttcgcatccg cgatatggtg aagcaattgg
     5461 agatcatctg taaaagtgaa gggattacct gtaccgaaga agctttgcat ctcgttgccc
     5521 agcgtgccga tggcgctatg cgcgatgcat taagtatctt cgatagaatc acatcttttt
     5581 ccggtaagtc aattgaagcg aaagatgtta ttgataattt aaatattctc gattacgaat
     5641 atttcttccg cattaccgaa tgcgctgtat cggaagatgc cacaactgct ctggtgctat
     5701 tcgacgaaat catcaataaa ggtttcgacg gcgatgtgtt tattaatggc cttgccgaac
     5761 attttcgcaa tctgctgatg tgtaaggaag aaaaaacgat aggattactc gacacatcag
     5821 aagctgcaat tgaaaaatat acctcgcagt cgcaaatgat tccggcttcc tttttattaa
     5881 gcggactgaa tttgttgaac agtgctgata tccaatatcg cggagcaaaa aataaacgct
     5941 tgcatgtaga gttggtgatc atgaaactat gttatattca taacgcagtt tcagctcttg
     6001 cttccggctc cgtggaaaaa aaaactcctg acctgaacaa aactgttcag ttaaagccta
     6061 aaattgaacc tcaacgagaa gcagaaccgc ttcctgtaaa tccctctttc cgaccggaac
     6121 ctaaatcaga agtgccgccg gtgcaaaaag tggtcgtgca gaataatgcc ataaatccac
     6181 ctgttccgga acaaatagaa acggtcatga tccctaaaaa aacggatctt cgacaatcag
     6241 tccgcaagga ggaagagcag cgcaaactgg aagtgccggt agtgcaagtc acacaagaaa
     6301 aaatcctgat tgagccggaa gatttttttg ttgcctggaa acagtttgcc gcatttatcc
     6361 gcgcagagaa gcaagcagaa gccattatta tggaccatgc agcagtttct atcgacgata
     6421 gaaatatagt taccgtgaaa ttagattcat cctatgaggt aaatatctta aagggcgtgc
     6481 acaacgaatt acacgacttt atcaagcaat cattgcaaaa taatactttc gaaatcaacc
     6541 tcgttgttga ggctgccaaa caggaaaaag tgaagcgtcc atatacagcg caagaaaaat
     6601 ataaaaaaat ggaagagcta aatcccctca tccgagattt aaaagaaaaa ttgggatttg
     6661 aattggatta ttaattttca taaaaagtag atgatcaata acatgaaact acatcgaatc
     6721 atttgtttag gattaggcgc aatagtgctt tctatcacaa gtctatctgc tcaaacggga
     6781 tcttcctcga gctcgtcctc cggaaatcag ggacagtcgg gaagcagcag tcaatccggc
     6841 tatataccta acagttcatc aagcagtgcc agtcaaggcg gcgctcccgg tcctattcgc
     6901 attgaaccca acacacctga tgccataact attttccaga ctacaaatgc atcgcccgga
     6961 gtaaacacgg ctaaggctac gaagtatgat ttcattaccg ttcccgacga tcctaccaga
     7021 acacgtattt atacgttgaa aaatggattg aaggtgtatc tgtctcaaaa taaagctgaa
     7081 cccagaattc aaacatatat tgctgttcgc acaggttcaa ataatgatcc gaaggaaaca
     7141 accggtcttg cgcattacct ggaacatatg atgttcaaag gcactaccag ctttggaacc
     7201 aataactggg atcgtgaaaa aatggtgatt gaaggtattt ctcaattata tgaaaaacat
     7261 cgccgtgaaa cagatcctga aaagaaaaaa gcaatctatg ctgaaattga taagctaagt
     7321 caagaagcag caaaattcgc catccccaac gagtatgaca aaatgatcac ctcacttggt
     7381 gcaaaaggta caaatgcata taccagcaaa gaacaaactg tttatgttaa tgatattccg
     7441 gccaatgaac ttgaaaaatg gttggtgatt gaaagtgagc gctttcgcac ttgtgtgttg
     7501 cgtttatttc atactgaatt agaagcagtg tatgaggaat acaatatggg acaagatcgc
     7561 gatggcagca aagtgtatga aacttattac cgcgcgctat tcccaacaac accttatggc
     7621 acacaaacta ccattggtga aggagaacat ttgaagaatc cttctatggt gaatatcatg
     7681 ggttacttct atacttatta tgtgccaaat aatatggctg tttgtttaag tggtgatctt
     7741 gattatgaca aaacaattgc actcattgat aaatatttcg gtgcttatgt aaataaacaa
     7801 gttggtgttc gcgagtttaa taccgaaaaa cctattactg aagtgcaacg tcgcgaagtg
     7861 tatggacaag aatcggcata tgtgcaatta ggatatcgcg ttccgggtgc aaatacagag
     7921 gataatatta ttgcgaaatt gattgcaggt atgttgtata atcagcaggc cggtttgctc
     7981 gacttagact tgaatcagca acaaaaagta ctggatgcag cagcctatca taccgaatgg
     8041 cgcgattata gtgttcttac cctggcagca gacccgcgtg atggtcagac tctagaagag
     8101 tgtgaacaat tattgcaggc agaactcgaa aagatcagaa agggtgagtt tgatgaatgg
     8161 ttgatgaaag ccgtgatcaa cgactatcaa ttatcacgca ccaaatcatt cgaatcaaat
     8221 tcaggtcgcg caggtgcatt tgtgaataca tttattcgtg gaattgattg gggtgcatac
     8281 ttgaatgaat acaatgtcat gtcaacaatc accaaagcag atattgttga atttgccaat
     8341 acctatttgg gaaaaaatag ttatgtagta gtgtataagc gcaaaggtga agacaagaat
     8401 attaaaaagg tagaaaaacc tgtaattaca ccgatagagc ccaatcgcga tatgtcaagt
     8461 aaattcctta catcgtttat ggaattacca agtagtcgcg tagctccgga attcgttgat
     8521 tacagcacta aaataagcaa caccatgctt aataatgatg tgccgatggc gtatattaaa
     8581 aacgaggaaa atcaactctt cgaattatac tacattctcg atatgggttc atataacgat
     8641 cgcgaactgg cattggcgat taattacttg ccttatttgg gtaccgataa atatagccct
     8701 gcgcaattgc agcaagagtt cttcaagtac ggactttcat ttacagtaaa tgccggaagt
     8761 gatagagttt atgtttcgct ttcaggactt gaatcaaacc tcgaaaaggg aattgaatta
     8821 tttgaacact tgctaagtca tgtgcaaccg gataacaagg cttatcgtga atatgtagac
     8881 ggtatcatca aggaacgtga ggatgctaaa acaaataaga actacatttt cggcgtggcg
     8941 atgctcaatt atgcaaagta tggtgccgtt tcgccacaaa ctgatatact cagcgaacaa
     9001 caattgcgaa atgtgttgcc cgcaacattg actgataaaa tcaaatcgct caccggatac
     9061 aaacatcgcg tgttttatta tggtcagatg cgtccggagc tcgtgcaaac aatgttgaat
     9121 aaataccatg taacaaaagc accgctcatg gattatccgc cggctactaa atatccggaa
     9181 aaggaaacca atgaaaataa ggtgctattt gtgcattatc ctatggtgca ggcgcaaatc
     9241 tcatttattt cgaaggacga aaatttcgat aaaaccgtgt tgcctcaggc gcgtatgttt
     9301 aacgaatatt ttggtagcgg tttgtcaagt attgttttcc aggaaatacg tgaaacaaaa
     9361 gcattggcat attcagccta tgcaaattat tctacaccac tgaataaaga tgaggcacat
     9421 tatgttcgtg catcggtgag tacgcaagca gataagatgc aagccgcgat ggaagccatg
     9481 cttgaaatca tgaataatat gcctcaggcg gaagaacaat tcaatgcgag tttggaatcc
     9541 gtgcttaagc aaatagaaag tgaaagaatt atccgctcca atattttctg gaattatgaa
     9601 agcatgaagc gcaaaggact gaattacgat tatcgtcgcg atgtttacat ggcagcgcga
     9661 ggaatgagca tgcaggatat gcaggaattt ttcaataatc atatcagcga ccgtcactat
     9721 actatcattg ttatgggcga ccgcaccaaa gttgacctga actatctcaa gtcgataggt
     9781 gctttccgtg agatgactat ggaagagatt tttggatatt gatatccact acctggcctc
     9841 cgaatagcaa tgttttatgc attggtattc ggaggtttta ggttttgcaa gggttatttc
     9901 cggggctatt tccccattcc ctccttcgaa gcccttacca gccttcagcg gctaatcaat
     9961 ttgtttctta atttcgcacc ataatacctc aaagaatatg gcagaagtga tcagaatgcc
    10021 ccgtatgagc gataccatga ccgagggcac cttcgtggaa tggcataaga aagtgggtga
    10081 caccgtgaaa attggcgata tcctggctga agtggaaacc gataaggcta ccatggagct
    10141 tgaaaactac gccaaaggaa cactgttgta tatcg
//
LOCUS       DAOQHY010000120        10166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_3334_length_10166_cov_5.294333, whole genome shotgun sequence.
ACCESSION   DAOQHY010000120 DAOQHY010000000
VERSION     DAOQHY010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10166
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3334_length_10166_cov_5.294333"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            347..1192
                     /locus_tag="PLL28_10245"
     CDS             347..1192
                     /locus_tag="PLL28_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNF69747.1"
                     /translation="MADIQQLEKIASQVRRDIVRQVYNCQSGHPGGSLGCTDFLVALY
                     FDVMQHDPKFNMNGSGEDMFFLSNGHISPVWYSVLARSGYFPVAELNTFRKLNTRLQG
                     HPTTAEHLPGIRMASGSLGQGMSVGIGAALSKKLNNGPHLVFTLHGDGELQEGQIWEA
                     AMFASHKQVDNIICTVDWNGQQIDGPTKKVMDLGDLHAKFSAFGWDIVMLEKGNDMAS
                     VVAALHEAKSRTGKGKPVMILMKTAMGAGVDFMMGSHEWHGIAPNKEQLEKAMAQLTE
                     TLGDF"
     gene            1274..3286
                     /locus_tag="PLL28_10250"
     CDS             1274..3286
                     /locus_tag="PLL28_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69748.1"
                     /translation="MRQKKKDRSAQLLEYSALSLAFIGLPAIADAQVVYVDVDPDTTT
                     YLDEYELDMDNDGSVDFKLIFDTDIFATFAPPELKLRINPLGSNSVAFLYQSIPVTYS
                     GMPFSTYIDGATAIFFTDVALLNDGDVIDNALNFQLEKAALYSRRFFFIYSESNYAAF
                     GGGYWNETTDHFAGFRLFTDGAYHYGWIRLNVSADSGITLLDYAYELNPETPLTAQVR
                     DYKISWVSVGDAGITETTADLAFSFNAAVDQADIASYRIFCVPTADVNDMTPDIAASL
                     SPDRYVEIIPDGSDVYSGNFSGILLDSNGDPIVEGVNYKLMAVNIMLNNYLPILSVPT
                     PKFSLVNTVQMPLNVTLSDLNNTGTGEDVRVKFDAPSITQGISSYRIIILPRDSADAF
                     TLDEALLVTPENFTEVAVGDADYAVMLNSTSTDAYGNLIYLEKYKAVVLSMADDVTVA
                     HDAMSDVSGVVVLETATAVASGVTLSDVNETASGFDLHVSFNYGTPEQTVATYQLYFV
                     DYATAFDFDVAAALASPNFISFTPDNVDFTFTGNDTLRDSDGDLISWGVPYYAWVLSV
                     SSDFGVSDTLSEPSNQVILNFPVGIETSDAATDQLSYSNGQFRVSCSEEQTLSFYNLS
                     GELISSQVIFPGITNLPVDLPAAMYLARLSNGKSLSVLPFVVTGTH"
     gene            3443..4819
                     /locus_tag="PLL28_10255"
     CDS             3443..4819
                     /locus_tag="PLL28_10255"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNF69749.1"
                     /translation="MKKLFLLVCIPLLIGMTAAVGRAQTSPPAATKRILILLDGSGSM
                     VDPWNGTNKWEVAKKLVTKTIDSIQRSEPGVEIGLRVFGFQQPRAVHDCKDSKLVVPI
                     GKNTGSAVKSALASITPQGYTPIAYSLFLAAGDFPTAEAANSIILITDGIENCDGDPC
                     ASTQVLRDKRITLKPFIIGLGIEEKDKKQFECVGAYYDASNEKTFANAMSIVISQALN
                     ITTTQINLLDAFGLPLEKNIEITLYDHASGELRYNYVHTPDDKQQPDTLFLNPVGKYD
                     ITVHTTPPIFLNDVELTPGKHNIIGIDVPLGTLVLSEGGNTTLSEKQLVLRDNETGVI
                     IYVQNFNTKMKYVAGTYDIDILTLPRLHYDNYEIKGGVESKIDIPVSGTLNITLTANT
                     TYSIYTVKNDQLEKVYENTMSAGMDPVQLLPGEYIVVSRSNIKKVAASTKEQRVQIKS
                     AKTTTLKL"
     gene            4821..6191
                     /locus_tag="PLL28_10260"
     CDS             4821..6191
                     /locus_tag="PLL28_10260"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNF69750.1"
                     /translation="MQKVITYRLKMMLLCILIATSSSLFSQGQTTRILIILDASRSMG
                     ENFNRTSKMQAARDIVGHIVDSLNGNPNIQLAMRVYGHQSPQPLNDCEDSKLEVAFRT
                     KNAATIKSRMETIRPQGVTPIAYSIEQSIADFGQDAKNYRNILILVSDGFESCGRNPC
                     EVVQRMRQQGILTKSYVIGIGIDALKYSEFSCMGEFTNISSEDQTEDVANSVIDKIFN
                     SVFVRVDLLDTENRPEETDAVMTFYDRSNHLPKFNYYHTINPKGNPDTITLDPAVNYD
                     MQVHTVPETWKRDVNLTSGKINVITQPAPQGYLRVDVRGETYKGKINCIIKKNGDIIN
                     AQQTEYAQKMLTGDYDVEILTIPVIYINHAQVEQDKTTTLEIAAPGFVTFSKNEALIG
                     GIYEYRDNKLTEIYELNEASLKEAVAIQPGKYKVIYRYASKKRMDATITVEFEVVSGT
                     SLTVRL"
     gene            6240..7199
                     /locus_tag="PLL28_10265"
     CDS             6240..7199
                     /locus_tag="PLL28_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNF69751.1"
                     /translation="MITIDYTNQKDTRSGFGAGILEAGRNNPNVVALCADLVGSLKLE
                     AFIKEFPERFVQCGIAEANMTGIAAGMTIGGKVPFACTFANFATGRGYDQIRQSIAYS
                     NKNVKIAASHAGLTLGEDGATHQICEDIGLMQMLPNFVVINPCDYNQTKAATIAAANY
                     EGPVYLRFGRPKWPIFYPADMPFEIGKAWKIQDGSDVSIFATGHLVLKAIEAAVILVE
                     KGYSVDLINIHTIKPIDAEAVIQSVSKTKCAVTAEEHQVIGGLGSAVGKVIAEHMPVP
                     IEFVGVQDTFGESGTPDQLLEKYGMSAAHIVEAALKAIGRKSA"
     gene            7263..7814
                     /locus_tag="PLL28_10270"
     CDS             7263..7814
                     /locus_tag="PLL28_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNF69752.1"
                     /translation="MSDQEIVQLCLDPLHRERGYRLLVSTYSKKIYWHVRRMVTDHDD
                     ANDVVQNIFIKVWKGLPEFRADSKLYTWLFRIATNESISFINEKYRRSSVALDNEQAS
                     HIQRLTQDAWFDGDDTQKQLQHAIDSLPDKQKQVFIMRYYDEMKYEDMEAILGTSTGA
                     LKASFHHAVKKVEQFLKAGLNRG"
     gene            7844..8443
                     /locus_tag="PLL28_10275"
     CDS             7844..8443
                     /locus_tag="PLL28_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69753.1"
                     /translation="MEQEQKNTLQELEDAGSTLPELLRRNTMRVPEGYFEGLEDRLFN
                     QVIAAEAPKSQTGKSLRMWMYISAAASVLIVAGIVFLQRNAANHSLEMQFAKMSDAEL
                     DNYINEQIASLSSDDIYNYLSEEIHSIEASDILSTELVGATDADEHFQEAMHEQVYPS
                     TENGTQGPAILDEELLEDIDDVLLQEYLNNAAIFEDMAL"
     gene            8484..8987
                     /locus_tag="PLL28_10280"
     CDS             8484..8987
                     /locus_tag="PLL28_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69754.1"
                     /translation="MKNYLIALLLCITGVAKAQGPPPPPPGMDDEKIEALRIAFLTKY
                     LDLSTDEAQKFWPVYNDMQEEMDVIKEKEKDLRKGKEIDKMTDEELNKMISSHFENEQ
                     KMLDIKKKYLEEFKKVLPLKKVALLADAENEFKREMMKHARDKQGPPPPGGGKPGGQQ
                     PTPDADH"
     gene            9097..9762
                     /locus_tag="PLL28_10285"
     CDS             9097..9762
                     /locus_tag="PLL28_10285"
                     /inference="COORDINATES: protein motif:HMM:NF025488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HNF69755.1"
                     /translation="MPYNTQLPQMILREYGRHIQNLVNFACKIEDDEVRNKLVQDIIE
                     LMGTMNPTLRNVEDFRHKLWDHIYMMSDFKLKANSPYPVPERETLTRKSIKLSYPKTK
                     IRFAHYGKNVETCVNKAVEMTDPELRQEYAQVIANYMKLVYQNWNSDNVNDNIIKQEL
                     ESLSHGAIKLEDDTDLDTLTRQNRGPQQGQQQRQQQHRHQGNNRNFKRNNGGNHKNFK
                     KNR"
     gene            9762..>10166
                     /locus_tag="PLL28_10290"
     CDS             9762..>10166
                     /locus_tag="PLL28_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNF69756.1"
                     /translation="MNAFEITGGRRLKGELIPQGAKNEALQVICAVLLTDKQVVIRNI
                     PDIRDVNFLIELLAKMGVKVNRMDANTCAFQADDVNHDFIHSEEFVNQSRKMRGSVMV
                     MGPLLARFKYATLSKPGGDKIGRRRLDTHVMGF"
BASE COUNT         2984 a         2075 c         2309 g         2798 t
ORIGIN      
        1 taacttactt atagccgcaa cccaatttcg ccaagcaccc caaatcatct gttaaagcga
       61 aacaaatgtt aatttttaag cgacctattg cttacaaatt tgcacttcca tcagcattcc
      121 ccattcacgg aaaaccgtta atccccgcat gtcacaaatt ctgtaaaatt taaagtgaaa
      181 atgcccatat ttcaacactt ccggctatta gcagcttgtt ttcctggtat ttgtgatgtc
      241 acatttcgcg attggccttg cgggtagttt tgccatcgcc ctacccacac ctgtgcaaag
      301 cgccttttgg ggaatgcctt atctttgccg aaacttaagc ttttacatgg ccgatataca
      361 gcaattggaa aaaatcgcat ctcaggtgcg cagagatatt gttcgtcagg tgtacaactg
      421 ccaaagcggt cacccggggg gctcattggg atgcaccgac tttttggtgg cgctgtattt
      481 cgatgtgatg cagcacgacc ccaagtttaa tatgaacggc tccggtgaag acatgttctt
      541 ccttagcaat ggtcatatct cgccggtatg gtatagcgtg ctcgccagaa gcggttactt
      601 cccggtggct gaactcaata ctttccgcaa gctgaatacg cgtttgcaag gacatcctac
      661 caccgccgag cacttgccgg gtattcgcat ggcttcaggc tcgctgggtc agggaatgag
      721 tgtgggtatt ggtgctgcgc tctccaagaa gttgaataac ggcccgcatc tggtgtttac
      781 cctccatggc gatggcgaat tgcaggaagg tcagatatgg gaagccgcca tgtttgcctc
      841 tcacaagcaa gttgataata tcatctgcac tgttgattgg aacggtcagc agattgatgg
      901 tcctaccaaa aaggtgatgg atctcggcga tctgcatgca aaattctctg ctttcggctg
      961 ggatatcgta atgcttgaaa aaggcaatga tatggcatct gttgttgctg ccttgcatga
     1021 agcgaaatcg cgcaccggaa aaggtaagcc tgtgatgatc ctgatgaaaa ctgccatggg
     1081 tgcaggggtt gatttcatga tgggcagtca cgaatggcat ggcatcgctc ctaacaagga
     1141 acagcttgaa aaagccatgg cacagctcac agaaacgctc ggcgatttct agtatatctt
     1201 tgaatgatta ataaagtcgc ggatttgtgc ggcttgcaat tacatcatca tgtcgttttt
     1261 gtaattttaa accatgcgac aaaagaaaaa agatcgttcc gctcaattgc tggagtattc
     1321 tgctctgtcg ctcgcattta tcggactccc tgcaattgct gatgctcagg tagtatatgt
     1381 tgatgttgat cctgacacta ccacttatct cgacgaatat gaactcgata tggataatga
     1441 tggcagtgta gattttaagc tgatttttga cactgatatt ttcgccacat tcgcgccacc
     1501 ggaattaaaa ctccgcatta accctctcgg cagtaatagt gttgcatttc tttaccaatc
     1561 aattcctgta acgtattcgg gaatgccatt ttctacctat atcgatggtg caacggcaat
     1621 ctttttcaca gatgtggcat tattgaatga cggtgatgtt atagataatg cgttgaattt
     1681 tcaattggaa aaagcagcat tatattcgcg acgatttttt ttcatctatt ctgaatctaa
     1741 ttacgctgca tttggcggtg gttactggaa tgaaactacc gatcattttg ccggcttcag
     1801 attattcacc gatggcgctt atcactatgg gtggatacgt ttaaatgtca gcgctgacag
     1861 tggcattaca ttgttggatt atgcctatga attaaatccc gaaacaccgc tcacagccca
     1921 ggttcgcgat tataaaataa gttgggtatc tgtaggagat gcaggaatta cagaaacaac
     1981 tgccgacctt gcattctctt tcaacgcagc cgttgaccag gctgatatcg catcgtatcg
     2041 tattttttgt gtgcctacgg cggatgtcaa tgatatgacg cctgacatcg ctgcttcgtt
     2101 atcgcctgat cgttatgttg aaattattcc cgatggcagt gatgtgtatt ccggtaattt
     2161 cagcggcata cttctcgaca gcaatggcga ccctattgtt gaaggtgtga attataaatt
     2221 gatggcagta aatattatgt taaacaatta cttgccgatt ttatctgttc ctactccaaa
     2281 attctcattg gtgaatacgg tgcagatgcc attaaatgtt acattatctg atttaaacaa
     2341 taccggcacc ggtgaagatg tgcgcgttaa attcgatgcg ccttcaatca cgcagggtat
     2401 cagtagttat cgcattatta tattgccgcg tgatagcgcc gatgctttta cattggatga
     2461 agcactattg gtaacacctg aaaattttac tgaggttgct gtcggtgatg cagattatgc
     2521 tgtaatgctt aattctacct caacagatgc atatggaaat ttaatttacc tcgaaaaata
     2581 taaagctgtt gttttatcta tggctgatga tgttaccgta gcacatgatg ccatgagtga
     2641 tgtgtcggga gttgtagtac tggaaacggc aactgctgtt gcatccggtg ttacgttgag
     2701 tgatgtcaat gaaacagcca gtggtttcga cctgcatgtt agttttaatt atggaacgcc
     2761 ggaacaaaca gtagcaacct atcagcttta ttttgttgat tatgcaacag cctttgattt
     2821 tgatgttgca gctgcgttgg catcgccaaa tttcatttct tttacgcctg ataatgtaga
     2881 ttttaccttc accggaaatg ataccctgcg cgacagcgat ggcgatctga tttcatgggg
     2941 tgttccctat tatgcctggg ttttatccgt ttcgagcgat tttggggtga gcgatacctt
     3001 atctgaacct tcaaatcagg tgatcctgaa cttcccggta ggtattgaga cttccgatgc
     3061 tgccacagat caactaagtt attccaatgg tcaattccgg gtgagttgct cggaagaaca
     3121 aaccctttcc ttctacaatt tgtccgggga gctgatttct tctcaagtaa tttttccggg
     3181 tattactaac cttcctgtcg acctgccggc tgccatgtac ctggcccgtc tgagcaacgg
     3241 aaaatcctta tctgtgctgc cttttgtggt taccggcact cactaatagg aggagttgaa
     3301 cagtatgcta tacacccttt cccgctagtg ttgatctgta ttttaacact tttggtcaaa
     3361 aacggcagca tttttgttta tttgcgtatg acccctccaa taaggcgctg aatttctgcg
     3421 ttttataaga ggcgcttttt acatgaagaa actctttctg cttgtctgta ttcctttgct
     3481 cattgggatg acagctgctg ttggtcgggc tcaaacctcg ccaccggcag ctaccaagcg
     3541 cattctcatc ctcctcgacg gctccggcag tatggtagac ccatggaatg gcacgaacaa
     3601 atgggaagtt gcgaaaaagc ttgttacaaa gactatcgac agtatacagc ggtctgaacc
     3661 cggtgttgaa ataggtttga gggtattcgg ttttcagcaa ccacgggcgg tgcacgattg
     3721 taaggactca aaattggtgg tgccgatcgg gaaaaatacc ggtagcgcag taaaaagtgc
     3781 cttggcatcg atcacaccgc aagggtatac gccaatcgct tattcactct ttttagccgc
     3841 cggagatttt cctacagcag aagctgccaa ctccatcata ctcatcacag atggtatcga
     3901 aaattgtgac ggcgatccat gtgcgagcac acaggtgttg cgtgataaac gcattacact
     3961 caagccattt attatcggcc tgggaattga agaaaaagat aaaaaacagt ttgaatgtgt
     4021 cggtgcctat tacgacgcat cgaatgaaaa aacatttgcc aatgccatga gcatagtaat
     4081 ttctcaggca ttgaatatca ctaccacgca gataaattta ttagatgcat tcggcttgcc
     4141 tttagagaaa aatattgaaa tcaccttgta tgaccatgcc agtggcgaac tgcgctataa
     4201 ttatgtgcat acccccgatg ataaacaaca acctgacact ttgtttttaa atcctgtagg
     4261 taaatatgac attaccgtac acactacacc accaatattt ttgaatgatg tggaattgac
     4321 gcccggtaaa cataatatta tcggaattga tgtgccatta gggactttgg tgttaagtga
     4381 aggtggaaat acaacattaa gtgagaagca attggtgctg cgcgataatg aaacaggtgt
     4441 gattatttac gtgcagaatt ttaataccaa aatgaaatat gtcgccggca cttacgacat
     4501 cgacatactt acactaccgc gtttgcatta tgacaattat gagatcaaag gaggtgttga
     4561 aagcaaaatt gatatacctg tatcaggaac attaaatatt acacttacag caaatacaac
     4621 atacagtata tatactgtaa aaaatgatca gttggaaaaa gtgtatgaaa ataccatgtc
     4681 ggcagggatg gatccggtgc aattattacc gggcgaatat attgtggtga gtcgctctaa
     4741 tattaagaaa gtagctgctt caaccaagga acaacgtgtg caaattaaat cggcaaaaac
     4801 aacaacatta aaactctgat atgcaaaaag taattacata tcgcctgaag atgatgttgt
     4861 tgtgtatact gattgcaacc tcttcatcat tattttctca aggtcaaact acgcgcatac
     4921 tgattatttt ggatgccagt cgcagtatgg gcgagaattt caatcgcacc tcaaaaatgc
     4981 aggctgcacg tgatattgtc gggcatattg tagatagttt aaacggcaac ccaaatattc
     5041 agctggctat gcgcgtatat ggtcatcaga gtccgcaacc attgaacgat tgcgaagatt
     5101 cgaaattgga ggtagccttt cgcacaaaaa atgctgcaac aattaaatcg cgcatggaga
     5161 ccatcaggcc gcagggagtt acgcctattg cttattctat agaacaatct attgccgact
     5221 ttggacagga tgcaaagaat tatagaaata tcttaattct ggtgagcgac ggatttgaat
     5281 cctgcggtcg aaacccgtgt gaagtggttc aacgcatgcg tcagcaaggt atactcacca
     5341 aatcgtatgt tatcggaata ggaattgatg ccttaaaata ttctgagttt tcatgtatgg
     5401 gtgagttcac caatatttct tcagaagatc agactgaaga tgtagcaaat agtgttatcg
     5461 acaaaatatt taattctgtg tttgtgcggg tagatctgct cgatacggaa aacaggcctg
     5521 aagaaacgga tgcggtaatg accttttatg acagaagtaa tcatcttccg aaattcaatt
     5581 attatcatac catcaatccg aagggaaatc ctgacaccat aacactcgac ccggcagtta
     5641 attacgacat gcaggtacat accgttcctg aaacctggaa gcgcgatgtg aatttgacat
     5701 caggaaaaat taatgtcatc acccaaccgg ccccgcaggg atatttgcgt gttgatgtaa
     5761 gaggtgagac ctataagggg aagatcaact gcatcatcaa gaaaaatggc gatatcatta
     5821 atgcgcagca aactgaatat gcgcaaaaaa tgctcaccgg cgattatgat gttgaaattt
     5881 taactattcc tgttatatac atcaatcatg cgcaggttga acaggataaa acaacaacgc
     5941 ttgaaattgc agctcccgga tttgtgacat tcagtaaaaa tgaagcatta atcgggggta
     6001 tttatgagta tcgcgataat aaactcaccg agatctatga attgaatgaa gcttccttaa
     6061 aagaagccgt tgccattcag cccgggaaat acaaggtcat ctaccgctat gcatcaaaaa
     6121 aacgtatgga tgctaccatt actgtggaat ttgaggtggt atcaggcact tcacttaccg
     6181 tcagactgta acttatcctt atcttcgcgc tgcaaacaat taattgaaaa ttcgcagacg
     6241 tgatcaccat tgattatacc aatcagaaag atacccgctc cggctttggt gccggcattt
     6301 tagaggcagg acgtaataac ccaaacgtgg tagcgctatg tgccgacctg gttggctcat
     6361 tgaaactgga agcttttatt aaagaattcc ctgaacgctt tgtgcagtgt ggtattgccg
     6421 aggcaaatat gaccgggata gctgccggaa tgaccattgg cgggaaagtg ccttttgcct
     6481 gcacatttgc aaatttcgca acaggaaggg gatatgacca gattcgtcag tctattgcct
     6541 attcaaacaa aaatgtgaaa atagctgcat cgcacgccgg acttacactt ggtgaagatg
     6601 gtgcaacgca tcagatctgt gaagacatcg gcttgatgca aatgctgccg aatttcgtgg
     6661 tgatcaaccc atgcgattac aaccaaacta aggctgccac cattgccgct gccaactatg
     6721 aaggtccggt ttacctgcgt ttcggtcgcc cgaaatggcc gattttctat cccgcagata
     6781 tgcctttcga gattggtaag gcctggaaga tacaggatgg cagtgacgtg tcgatattcg
     6841 caaccggaca cctcgtatta aaagccattg aagcagctgt tatccttgtt gagaaaggat
     6901 atagtgttga cctgattaat attcatacca ttaaaccaat cgatgccgaa gcagtcatac
     6961 aatcggttag caaaacaaag tgcgcagtaa ctgccgaaga acatcaggtg ataggcggat
     7021 tgggaagtgc cgttggaaaa gtaattgctg aacatatgcc cgtgccgatt gaatttgttg
     7081 gcgttcagga taccttcggc gaaagcggaa cacctgatca gttgctcgaa aagtatggca
     7141 tgagtgctgc tcacatagta gaagctgcat tgaaagccat aggcagaaag tcggcttaac
     7201 ttcaacccga aattttccga ttgcgatcgg tagggtgctg accaaacacc aatttaccgc
     7261 ggatgtcaga ccaggagatc gtacaattat gccttgaccc actccatcgc gagcgtggtt
     7321 atcgattgtt ggtatccacc tattcaaaaa aaatttattg gcatgtccgt cgcatggtaa
     7381 ctgatcatga cgatgcgaat gatgttgtgc agaatatttt cattaaagtg tggaaaggac
     7441 ttcccgaatt tcgtgccgac agcaaattgt atacctggct gtttcgcatt gccaccaatg
     7501 agtctatatc ttttattaat gaaaaatatc gccgtagcag tgtagcgctg gataatgaac
     7561 aagcgtcgca tattcaacga ttgacacaag atgcctggtt cgatggtgat gatacacaga
     7621 agcagttgca gcatgccatc gacagtttgc ccgacaagca aaagcaagtg tttatcatgc
     7681 gttattatga tgaaatgaaa tatgaggata tggaagcgat tttgggcact tcaacaggtg
     7741 ctctgaaagc cagttttcat catgcagtaa aaaaagttga acaattttta aaggccggat
     7801 taaaccgtgg gtgattatat atatctaatc aaacattaac agtatggaac aggaacaaaa
     7861 aaatacactg caggaactgg aagatgccgg aagcaccctc cctgaactgc tcaggcgcaa
     7921 taccatgcgt gtgccggaag ggtattttga agggctggaa gacaggctgt tcaatcaggt
     7981 gatagctgct gaggctccga agtcgcaaac ggggaaatcg ctgcgaatgt ggatgtatat
     8041 atctgctgct gcgtcagtac tcattgttgc cggaattgtt tttttacagc gcaatgccgc
     8101 taatcattca ctggaaatgc aattcgcgaa aatgagtgat gctgaattgg ataattatat
     8161 caatgaacaa attgcatcgt tgagtagtga tgatatttat aattatttga gtgaagaaat
     8221 tcatagtata gaagcttctg atattttgtc gacagaatta gttggtgcaa cagatgccga
     8281 tgaacatttt caggaggcta tgcatgagca agtatatcct tcaacagaaa atggaacgca
     8341 aggtcccgca attttggatg aagaattgtt ggaggatata gatgatgtgt tattacaaga
     8401 atatttaaat aatgcagcca tttttgaaga catggcttta taattcgaaa tgttacctaa
     8461 cctcaaacca taaattgacc attatgaaaa attatttgat cgctttactc ttgtgtataa
     8521 caggagtggc taaagctcaa ggtccgccac caccacctcc gggtatggac gatgaaaaaa
     8581 ttgaagcttt gcgcattgct tttctgacaa aatatctcga cctcagcact gatgaagcac
     8641 aaaaattctg gccggtatat aatgatatgc aggaagagat ggatgtaata aaagaaaagg
     8701 aaaaagatct tcgcaagggc aaagaaattg ataaaatgac cgatgaagag ctgaataaaa
     8761 tgatcagcag tcattttgaa aatgagcaga agatgcttga tatcaagaag aagtatctcg
     8821 aggaattcaa aaaggtattg cctttgaaaa aggtggcctt actggcagat gccgagaacg
     8881 aatttaagcg tgagatgatg aaacacgccc gcgacaagca gggacctcct cctccgggag
     8941 gcggcaagcc cggtggacag caaccgactc cggatgccga ccattaaccc ctaatttgaa
     9001 gtacaagctg cgggcgctct gagaagggtg cccgcagtgt tttgggcact tgcttactgt
     9061 ggtgcaatct gctacctttg ttgcaaatac aattcaatgc catataatac ccagttgccc
     9121 cagatgatat tgcgcgaata cggtcgccat atccaaaacc tggtcaactt tgcctgcaag
     9181 attgaagatg atgaagtgcg caataaactt gtgcaggata taattgaact gatgggaact
     9241 atgaatccca ctttgaggaa cgttgaagac ttccgtcata aattatggga tcatatttat
     9301 atgatgagtg atttcaaatt gaaagcaaac tctccatatc ctgttcctga acgcgaaaca
     9361 ctaacgagaa aaagtatcaa gttaagttat ccgaaaacaa agattcgctt cgcacattac
     9421 ggaaaaaatg tagagacctg tgtgaataaa gctgttgaga tgacagatcc tgaattgcgt
     9481 caggaatatg ctcaggtaat tgccaattat atgaaactcg tttaccaaaa ctggaatagt
     9541 gataatgtga atgacaatat catcaaacag gaattggaga gtttaagtca tggtgctatt
     9601 aaattggaag acgatactga tctggataca ttgacacgtc agaatcgcgg accgcaacaa
     9661 ggacaacaac agcgccaaca gcaacatcgt catcagggca ataaccgcaa cttcaagcgg
     9721 aataatggcg gcaaccacaa gaatttcaaa aagaatcgtt gatgaacgca tttgagatca
     9781 ccggaggaag gcgcctaaag ggtgagttga ttcctcaggg ggcaaagaat gaggcactgc
     9841 aagtgatatg tgccgtttta cttacagata agcaggtcgt tatccggaat attcccgata
     9901 tccgcgatgt caacttcctg attgaactgc ttgcaaaaat gggtgtgaag gtaaatcgca
     9961 tggatgccaa tacctgtgct tttcaggctg atgatgtcaa tcatgatttt attcacagcg
    10021 aagaatttgt gaatcaaagt cggaaaatgc gcggctcagt tatggtaatg ggaccattgc
    10081 ttgcgagatt taaatacgcc acactttcta aacccggagg cgataaaatc ggtcgccgca
    10141 ggttagatac ccatgtcatg ggattt
//
LOCUS       DAOQHY010000121        10132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_3350_length_10132_cov_5.353677, whole genome shotgun sequence.
ACCESSION   DAOQHY010000121 DAOQHY010000000
VERSION     DAOQHY010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10132
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3350_length_10132_cov_5.353677"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PLL28_10295"
     CDS             <1..298
                     /locus_tag="PLL28_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF69757.1"
                     /translation="TTYYILFACYGIYAAATEGIAKAWITNITPKEEAGTAIGTYTGL
                     SSIAAFFASALAGLIWSVAGAGWAFGISAAVALLVGAYLVSIPFQRVVSSANGA"
     gene            405..1724
                     /locus_tag="PLL28_10300"
     CDS             405..1724
                     /locus_tag="PLL28_10300"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HNF69758.1"
                     /translation="MSLYKKILNGNWTTTINLAIRGATIIGKFLLVYFLAKTLTVEQY
                     GVWGIFQTSISLSLYVVGLDFYTYSSRRILDYPVEERSPMLRDQLVFYFISYLVIFPI
                     LGLLFVFNEIDWRFAIFFYAILLLEHLAQETYRTFVVFSKPIIANIVLFLRTGLWPFV
                     LLFMWFIGFDELRNLKAVYLFWMAGGLSAAAVSIYFISKIKFKPVKNIPIDWKWIKQG
                     IGVSLIFFVGTVGYKIIEFADRYFVDYYHSKEDVGVYTFYAGICNIVETFVHTAVIII
                     FSPKLIETFHKDNYQYRKTFGTFARQVVGATLLVGVSLALLGIPLLRSLGESEYIEDY
                     NVFLLLVLSKMVLNISLIFHYILYVRKNDFPIIKATIYASVINILLNFILIPPLSIMG
                     AAIATLVSFMIILVMKVYYTRHLPEARQIIYLRFLRRKLRRKANTNH"
     gene            1736..2926
                     /locus_tag="PLL28_10305"
     CDS             1736..2926
                     /locus_tag="PLL28_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69759.1"
                     /translation="MEKAYYEHIQWWDYLIYLVYNGLLAYLLGRVGEIALNIGKQARV
                     LFFIAFEVYFLLVIGDSLFHYLPYLPDSELYSYMISSGQYPETSSENLIVIYYISIFL
                     SIICLNDPVIFTFFCIFLYALGAMLFIQAWKKADPSFNTNSETSAALLFLLWPAAALY
                     ITTPLREAFIMFGFGLFLNGFMDCIYKGKWRSVIIGSIIICAIRLQLFVFVLPVLGAL
                     VVYKSSLHKIWKSAILVGTIVAAFLVVRYVLVEHPLSPQTMAQLRNVNLDNAGPLGYG
                     RVDWDSYTDMARDYPFIIAQFMFSPFPIFVQHDPLSTFVPFLDCLFLIFLLILIFSRF
                     KALIRQHLPVLLLCVFYIILFGGYEYHITGAVRHRMPLEALFILMAAPALSRIIFRKK
                     HAAA"
     gene            2913..3725
                     /locus_tag="PLL28_10310"
     CDS             2913..3725
                     /locus_tag="PLL28_10310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF69760.1"
                     /translation="MPQLSILLSTINDRIFQAETILNTPLPDTEFVVVHQLTEGNVAE
                     NYQSFYARFTEKPVRFIQQSAKGTGRSRNAALRAGSGNLFYLCDDDLILEPEFLDRIL
                     QASTQYPNADIFTFQIRTTEGKPYKNYPDKPYQHTLRSTATVSIVEMVIRKSAVDNEM
                     IAFDERFGLATDYNTGEEFVMLHDALTKGAVAMYIPQVLVQHPPVSSGKIYNKTVAFE
                     KGAMIARVYDWRWPLINLAFAVRKYKEYRHSAGFFAFLIAMYKGSMHFNKND"
     gene            3718..4476
                     /locus_tag="PLL28_10315"
     CDS             3718..4476
                     /locus_tag="PLL28_10315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNF69761.1"
                     /translation="MTEPTVSVIMPVYNAAKYLQQAVDSVKSQTYANWELWMVNDKST
                     DNSAEIMSQLASGDARIHAIHLENNSGSAHARNIAIAQAKGKYIAFLDADDQWKPEKL
                     KLQVAFMQQHNAVLSHTAYDTISASNESLHREIHVNAKVTYRQLLKNNTIGCLTAMYD
                     AEALGKQFMPDLRKRQDYGLWLQILRNGRVAYGLDEVLATYRVGMDSLSGNKWRVLRY
                     NWMLLRKHEQLSLISSTYYFLCFLWNKTFKYLGK"
     gene            4473..5147
                     /locus_tag="PLL28_10320"
     CDS             4473..5147
                     /locus_tag="PLL28_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69762.1"
                     /translation="MKRAIIFGSSGLTGNALAKLIAEDPRYGELICFQRREVAIQYPK
                     QKNVITTFQDLHYYADLFYGTDVFCCLGTTMRNVNNDKVAYREADVDRPLAAAKAAAS
                     HGATSFTIQTALTANAKSMVFYNRIKGEVEDAVRNLGLRAVYIYRPSILTGNRQEKRT
                     GEGMGITITKFVTPILVGPWKKYRAIAAADVAKAMLIVAKSEKPGDHIFLSDRIQAIA
                     DGSTWQ"
     gene            5128..6060
                     /gene="queG"
                     /locus_tag="PLL28_10325"
     CDS             5128..6060
                     /gene="queG"
                     /locus_tag="PLL28_10325"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNF69763.1"
                     /translation="MAARGNENYTRLVKQLSAELGFSYCGISTATELTEEAHQLEKWL
                     SKGMHGAMGYMAEHFDKRIDPRKLVPGARSVISLMYNYYPEKTLDDEGNYHISKYAYG
                     EDYHTVIKEILYQLLDRLKENIGEINARVFVDSAPVLEKAWAKRSGIGWQGKNTNIIH
                     PKSGSFFFLAEIISDLDLIPDGPIKDHCGTCTACIDACPTEALTPYHIDATKCISYLT
                     IELRDAIPLSFAGKMNDWMYGCDICQDVCPWNRFSKHHQQERFLPSDDLQQMQKSDWQ
                     EITEDIYRKLFKGSAVKRAKFVGLKRNIAFLNGE"
     gene            complement(6457..7038)
                     /locus_tag="PLL28_10330"
     CDS             complement(6457..7038)
                     /locus_tag="PLL28_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HNF69764.1"
                     /translation="MLGLKLPTDPRWVNLAEKSLEEILTDHAYCEQKAASSCISIIQL
                     FPEYERLVDEVSPVVTEEWAHFRLVLKELKDRGLKLGKQRSDRYVHELSKFQLKGGTR
                     QQQLLEKLLMAAMIEARSCERFRLLSVGLSDEKLRQFYHGFMVSEAGHYRMFLDLANA
                     YIDSEIVKTRWQQWLDYEAELMTRLLPDGKLMH"
     gene            7065..8054
                     /locus_tag="PLL28_10335"
     CDS             7065..8054
                     /locus_tag="PLL28_10335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNF69765.1"
                     /translation="MPLKILVLRFSSIGDIVLTTPVLRCIKEQLPDCELHYATKKAYV
                     SLLSANPYIDKIHAFEEKKLPALMDALDAEKFDFIADLHHNLRTFLITSHLGLPNRKF
                     NKLNWQKWLMVKWKINKLPQQHIVDRYLKTVEPLGVKNDGKGLDHFIPADAHVAISSL
                     PQEFQSGYIALVIGAMHATKKMPPVKLVELVDRCSLPVVLIGGPDDVAAAEIIAQQAR
                     NKVYSACGKYNLQQSASLLQQSIKVYAHDTGMMHIAAALKKDVISIWGNTIPEFGMYP
                     YFGTAYTIAQQRADGKILEVATLSCRPCSKLGYQSCPKGHFRCMLDIDLSGVK"
     gene            8134..9486
                     /gene="purB"
                     /locus_tag="PLL28_10340"
     CDS             8134..9486
                     /gene="purB"
                     /locus_tag="PLL28_10340"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNF69766.1"
                     /translation="MDERALMSISPIDGRYQNKVTELSNYFSEYALMKFRIYVEVEYF
                     IALTEMPIPQFKNFEADNRSRLRKIYKKFNLDDCKVIKSIEEKINHDVKAVEYYIKQE
                     FDALGLSEYKEFIHFGLTSQDINNTAIPISFRDALIDVYAPLLEELINTLSELAVQWT
                     KIPMLARTHGQPASPTMLGKEIRVFIERLGKQRNLFNTIPYSAKFGGATGNFNAHFVA
                     YPDIDWILFADEFVSSRLGLERQRYTTQIEHYDNLAAAFDGLKRINTILLDLSRDMWT
                     YISMDYFKQRINKNEVGSSAMPHKVNPIDFENAEGNFGMANALFEHLAAKLPISRLQR
                     DLTDSTVLRNIGVPMAHTLIAMKSVLKGLKKLQVNEQRIHDDLDNNWAVIAEAIQTIL
                     RREGYPQPYEALKELTRNGQAITKDTLQSFINGLDVSDDVKQQLLGITPFNYTGIRFF
                     "
     gene            9508..9684
                     /locus_tag="PLL28_10345"
     CDS             9508..9684
                     /locus_tag="PLL28_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HNF69767.1"
                     /translation="MSGGKLIEGEDYYINAEGLFVFTAAFLLKRGFCCGNGCAHCPYP
                     KSEENTYPVEEKKP"
BASE COUNT         2913 a         2250 c         2088 g         2881 t
ORIGIN      
        1 aaccacatac tatatactat ttgcttgcta tggcatatat gccgctgcaa cagagggtat
       61 agccaaggca tggatcacca atatcacacc caaggaagag gcaggaactg ctataggcac
      121 ctacacagga ttaagtagta ttgcagcctt cttcgcatca gcgctcgccg gactgatctg
      181 gagtgttgca ggagcgggat gggcctttgg aatcagtgct gccgtggctt tactggtagg
      241 tgcatacctt gttagcatcc catttcaacg tgttgtcagc agcgctaacg gagcgtaaaa
      301 gtgcgtatct tagcccccta aacatgccac cgccgtatgg atgtcagccc gaaacgctgc
      361 ataactgttt caatcgccgg aagtaagatc gataggtaag aacaatgtcg ctttacaaaa
      421 agatcttgaa cggaaattgg actacaacca ttaacctcgc cattcggggt gctaccatca
      481 ttggtaaatt cctgctggtt tattttctcg caaaaacact taccgttgag caatatggtg
      541 tttggggtat ctttcaaact tctatcagct tatcactcta cgtagtaggt ctcgattttt
      601 atacttattc atcgcgccgc atcctcgatt atccggttga agaacgcagc cctatgttgc
      661 gcgatcagct cgtattttat tttatcagct atctcgtgat ttttccaatt ctcggattgc
      721 tgtttgtatt caatgaaata gattggcgat ttgcaatttt cttttacgcc attctcctgc
      781 ttgaacattt agcccaggag acgtatcgta cattcgtagt tttcagcaaa cccatcattg
      841 cgaatattgt attgttcctg cgcaccggat tatggccatt tgtactgtta ttcatgtggt
      901 tcattggctt cgatgaattg cgcaacctca aagcagttta cctgttctgg atggccggag
      961 ggctctctgc tgctgcagtt tcaatttact tcatatctaa aataaaattt aaaccggtca
     1021 aaaatattcc catcgactgg aaatggataa aacaaggtat tggcgtaagt ctcatatttt
     1081 ttgtcggaac agtaggatac aaaatcattg aatttgccga ccgttatttt gtagattatt
     1141 atcatagcaa agaggatgtc ggagtttata ccttctatgc cggtatatgt aatatcgttg
     1201 aaacttttgt gcatactgcg gtgatcatta ttttctctcc caagctgatt gagacatttc
     1261 ataaagacaa ttatcaatat cgcaaaacct ttggcacctt cgcccgtcag gtggtaggcg
     1321 ctactttgct ggttggggtt tcactggcat tgttgggaat accattgctg cgctctttag
     1381 gtgaatcaga atacatcgaa gattataatg ttttcctact gctggtatta tcaaaaatgg
     1441 tattgaatat ctccctgata ttccattaca tcttgtatgt gaggaaaaac gatttcccta
     1501 ttatcaaggc taccatttat gccagcgtga tcaatatatt actcaacttc attctcatac
     1561 caccactaag catcatgggt gccgccatag caacactagt cagtttcatg attatattgg
     1621 tgatgaaagt ttattacacc cgacatcttc cggaagcacg gcagatcata tacctccgct
     1681 ttttaagacg aaaactgcgt agaaaagcaa atacaaacca ttaatacgat ctcgtatgga
     1741 gaaagcctat tatgaacata tccaatggtg ggattacctg atatatctgg tctacaatgg
     1801 cctgcttgcc tacctgttgg gtcgcgttgg cgaaatcgca cttaatatcg gcaaacaagc
     1861 aagagtgttg ttcttcatcg catttgaagt gtatttcctt ttggtgatag gagattcgct
     1921 gtttcattac cttccttacc ttccggattc ggaattgtat agttatatga tctccagcgg
     1981 tcaatatcct gaaaccagtt ccgaaaacct gattgtaatt tattatatct ccatttttct
     2041 tagcatcatc tgtctcaacg atcctgttat atttaccttc ttctgcattt ttctgtatgc
     2101 acttggggcg atgttattta ttcaggcatg gaaaaaagct gatccctcat ttaatactaa
     2161 cagtgaaaca agtgcggccc tgttattttt attatggccg gccgcagcat tgtatatcac
     2221 cacgccattg cgagaggcat ttattatgtt cggttttgga ttattcctca acggattcat
     2281 ggattgcatc tacaaaggaa aatggcgctc tgtgattatc ggctccatca ttatttgcgc
     2341 tattcgactg caattatttg tatttgtgct gcctgtttta ggcgcactcg ttgtctacaa
     2401 atcatcactc cataaaatct ggaaaagcgc tatcctcgtg ggcacgatag tagcagcatt
     2461 tttagtggtg cgctatgtat tggttgaaca tccactcagt ccgcaaacaa tggctcaatt
     2521 gcgcaatgta aatcttgata acgccggacc attgggatac ggtcgcgttg attgggatag
     2581 ctataccgac atggcccgag actatccctt catcattgca caattcatgt tcagtccctt
     2641 ccctattttc gtgcaacatg atccattgtc gacatttgtc ccttttctag attgcctatt
     2701 tttgatattt ttgttgatat tgattttttc gagatttaaa gcacttatcc gtcagcatct
     2761 accggtgctt ttactctgtg ttttttatat cattttgttc ggcggatacg aatatcatat
     2821 cacaggagcc gtacgacacc gtatgccctt ggaagcatta ttcattttaa tggcagcacc
     2881 tgcattgagc cgaattatat tcagaaagaa acatgccgca gcttagcata ctcctttcaa
     2941 caattaacga taggatattt caggcagaaa ctattctcaa tacccctttg cctgacaccg
     3001 agtttgttgt tgtgcatcag ctcactgaag gaaatgtagc agaaaattat cagtcattct
     3061 atgcgcgttt taccgaaaag cctgttcgat ttattcaaca atcggcaaaa ggaacaggtc
     3121 gcagcagaaa tgcagcactc agggcaggaa gcggcaattt attttatttg tgcgacgacg
     3181 atctgatatt ggaacctgag tttctcgatc gtattttgca agcctcaaca caatacccaa
     3241 acgctgatat ttttactttt caaattcgca ctaccgaagg taaaccatac aaaaattatc
     3301 ccgacaaacc ctatcaacac accttgcgca gcacggccac cgtttcgatt gttgaaatgg
     3361 taatcagaaa gtcggcggtt gacaatgaga tgatagcttt cgatgagcgc tttggcttag
     3421 caacagatta taataccggt gaggaatttg tgatgttaca cgatgcctta acaaaaggtg
     3481 ctgttgcgat gtacattcct caggtattgg tgcaacatcc tcctgtcagc tcaggaaaaa
     3541 tttataataa aaccgtggca ttcgaaaaag gagcaatgat agcacgcgtg tatgattggc
     3601 gatggccatt aattaatctg gcatttgccg tgcgcaagta taaagaatat cgacattcag
     3661 caggattttt cgcatttttg attgccatgt acaaaggcag tatgcatttc aacaaaaatg
     3721 actgaaccaa cggtttccgt tatcatgcct gtttataatg cagcgaaata tctgcaacag
     3781 gccgttgact ccgtcaaatc gcaaacgtat gccaattggg aattgtggat ggtgaatgat
     3841 aaaagcactg acaactccgc agaaatcatg tcgcaactgg cctccggcga tgcacgtatt
     3901 cacgcgattc atctcgaaaa taatagtggt tctgcacatg ccagaaatat tgccatcgcg
     3961 caagcaaagg gaaaatacat tgctttttta gatgccgatg atcaatggaa acccgaaaaa
     4021 ttgaaattgc aggtggcatt catgcaacaa cacaatgctg tactttcaca tactgcctac
     4081 gataccattt ctgcttcaaa cgaatctctg catcgcgaaa ttcatgtcaa tgcaaaggtt
     4141 acctatcgtc aattattaaa aaataatacc atcggatgcc tcactgccat gtatgatgca
     4201 gaggccttag gaaaacaatt tatgcccgat ctgcgcaaac gtcaggatta tggcttatgg
     4261 ttgcaaatat tgcggaatgg acgagtagct tacggtctcg atgaagtgct tgccacctat
     4321 cgagtaggta tggacagcct ttccggcaac aaatggcgcg tgctgcgata caattggatg
     4381 ctgttgcgca aacatgaaca attatcatta atttcatcca cgtattactt tctttgtttt
     4441 ctgtggaata agacatttaa atatttaggc aaatgaaacg tgcaataatt ttcggatcca
     4501 gtggcttgac aggaaatgca cttgcaaaat tgatagctga agaccctcgc tatggtgaat
     4561 taatttgctt tcaacgcaga gaggtcgcca tacaatatcc taaacaaaaa aatgtgatta
     4621 ccacatttca ggatttacat tattatgccg acttgtttta tggcactgat gtattctgtt
     4681 gcctcggaac aaccatgcga aatgtcaata atgataaagt tgcttatcgc gaagccgatg
     4741 tcgaccgacc actagccgct gcaaaagccg cagcttcgca tggcgcaact agttttacaa
     4801 tacaaactgc acttacggca aatgccaaat cgatggtatt ttacaacagg ataaaaggtg
     4861 aggttgaaga cgctgtcagg aatcttggtt tgcgtgctgt ttatatatat cgtccaagca
     4921 ttctcaccgg aaaccgacaa gaaaaaagaa caggcgaagg catgggaatc accatcacca
     4981 aatttgtgac tccaatactt gttggtccct ggaaaaaata ccgcgcaatc gctgccgctg
     5041 atgtggcaaa agctatgttg atagtggcaa aatcagaaaa accaggcgat catatttttc
     5101 tttccgatcg cattcaagca atcgccgatg gcagcacgtg gcaatgaaaa ttatacccga
     5161 cttgtcaaac aactttctgc tgaattagga tttagctact gcggcatttc cactgcgaca
     5221 gaactcaccg aagaagccca tcagcttgaa aaatggttgt cgaaaggaat gcacggcgcc
     5281 atgggctata tggctgagca tttcgacaag cgcattgacc cgcggaaact tgtgccggga
     5341 gcgcgctctg ttatatcatt gatgtataat tattatcctg aaaaaacatt agacgacgaa
     5401 gggaattatc atatctccaa atatgcctac ggagaagatt atcataccgt aataaaagaa
     5461 attctttacc aattactcga tcgcttgaaa gaaaatatcg gcgaaattaa cgcacgcgtt
     5521 tttgtcgact cggctcccgt gctggaaaaa gcctgggcta aacgaagcgg tattggctgg
     5581 cagggaaaaa ataccaatat cattcatccg aaatccggct cttttttctt tcttgctgaa
     5641 ataatctcag atttggattt aattcccgac gggccaataa aagatcattg cggcacatgc
     5701 accgcttgta ttgatgcttg tccgacagaa gcactcactc cctatcatat cgatgcaaca
     5761 aaatgtatca gctaccttac cattgaactg cgtgatgcaa ttccattgtc atttgcagga
     5821 aaaatgaacg attggatgta tggttgtgat atttgtcagg atgtatgtcc atggaatcgc
     5881 ttttcaaaac atcatcaaca agaaagattt ttgccatccg acgatttgca acaaatgcaa
     5941 aaatccgatt ggcaggaaat cactgaagac atctaccgca aactattcaa aggctcagca
     6001 gttaaacgag cgaaatttgt tgggttgaag cggaatattg cgtttttgaa tggggagtaa
     6061 tcgatttttt ttcacgcaga ggcgcagaga cgcagagcaa agcaaagtat caggtatcaa
     6121 gtatcaggta tcaggtaaaa attcacgact cacgagtcac gtttcacgac attagtatct
     6181 agaaccaagt atctagtatc tagactaggt atcaggtaaa aattcacgat tcacgacatt
     6241 agtatctaga accaagtatc tagtatctag actaggtatc agattataat tcacgattct
     6301 cgattctcga ctcacgattg gtagtgagta gtgggaatag cccggttggc actgtaactt
     6361 cgttacagta taattattca catgatttct gaaattcact ccctctccct ctgggagagg
     6421 gccggggtga gggcactcac ccactcaccc ctcccctcag tgcatcaatt tcccatccgg
     6481 aagcaatctc gtcatcaatt ccgcttcata atccaaccat tgctgccacc ttgtttttac
     6541 tatttccgaa tcaatgtaag catttgcaag atccagaaac atgcgatagt gccccgcttc
     6601 gctcaccatg aaaccgtggt agaattgacg cagtttttca tccgataacc ccacactcaa
     6661 caagcggaaa cgctcacaac tcctggcttc tatcatcgcc gccatcagga gtttttcaag
     6721 caactgttgc tgcctggtgc cgcccttcag ctgaaattta ctgagctcat gcacatagcg
     6781 gtctgatcgc tgcttgccaa gctttaatcc tctatctttc aattctttca gaactaatcg
     6841 aaagtgtgcc cattcttcgg ttaccacagg cgaaacctca tcaaccaaac gctcatattc
     6901 cgggaataat tggatgatgc tgatgcaact cgacgctgcc ttttgttcac aataagcatg
     6961 gtcggtcaag atctcttcca ggctcttttc tgccaggttg acccaccgcg ggtcggttgg
     7021 taactttaat cctaacattt cgcaactttg ctgcaaattt acacatgccg ctaaagattt
     7081 tggtcttgcg ttttagttct atcggcgata ttgtgctcac cactccggtg ctgcgatgta
     7141 tcaaagagca attgcccgat tgcgagctgc actacgctac aaaaaaggca tatgtttctc
     7201 tgctctcggc aaatccatac atcgataaaa ttcacgcctt cgaagagaaa aaattacctg
     7261 ctctgatgga tgcgctggat gcggaaaaat tcgattttat tgccgacctc catcataatt
     7321 tacgcacatt tctcatcacc tcccacctcg gactgccaaa tcgcaaattc aataaactga
     7381 attggcagaa atggctgatg gtgaaatgga agatcaataa actgccgcaa caacatatcg
     7441 tagaccgtta tctcaaaact gttgaaccac tgggtgtgaa aaatgatggc aaaggacttg
     7501 atcattttat tcccgccgat gcgcatgttg ctatttcttc tttaccgcaa gaatttcaat
     7561 cgggatatat cgcactggtg ataggtgcca tgcatgctac caaaaaaatg cctcccgtaa
     7621 agcttgttga actggtagat cgctgttcat tgcctgtggt gttgattggt ggacctgatg
     7681 atgtggctgc tgcagaaata atcgcacagc aggcaagaaa taaagtttat tccgcctgtg
     7741 gaaaatacaa tttacaacaa agtgcttcac tcctccaaca atctataaaa gtgtatgcgc
     7801 atgataccgg catgatgcat attgcagcgg cattgaaaaa ggatgtgatc agcatctggg
     7861 gaaataccat tccggaattt ggcatgtatc cttacttcgg aactgcatat accatcgccc
     7921 aacaacgcgc cgacggcaaa atactcgaag ttgcaaccct ttcctgccgc ccttgctcaa
     7981 aactgggcta ccaatcctgc cccaaaggcc atttccgctg catgctggac atcgacctct
     8041 ccggggtgaa atagcctgca atgcaacccc tgtgcggact ttaccctctt ttcatcgtat
     8101 attatgtaat tttgcagtct aaatgcaggc aagatggatg agcgcgcgct gatgagtatc
     8161 tccccgatag atggtcgtta ccaaaataaa gtgacggaac tgtctaacta tttctcggaa
     8221 tatgccctga tgaaattcag gatttatgtc gaggtggaat actttattgc cctcaccgaa
     8281 atgcctattc cacaattcaa aaattttgaa gccgataacc gctcgcgact aagaaaaatc
     8341 tacaaaaaat tcaatctcga cgattgcaag gtgatcaaat ctatcgaaga aaagatcaat
     8401 cacgatgtta aagcagtgga atattatatc aaacaagaat ttgatgcatt gggattgagt
     8461 gaatacaaag aattcattca tttcggatta acctcgcaag acattaataa tactgccatt
     8521 cctatcagct tccgcgatgc attgatcgat gtatacgcac ccttgctgga ggaattgatt
     8581 aatacattaa gtgaactcgc tgtgcagtgg actaaaattc ccatgctcgc gcgaacacat
     8641 ggtcagcctg catcaccaac catgctcgga aaagaaattc gtgtgttcat cgagcgattg
     8701 ggcaagcaac gcaatttatt caatactatt ccttacagcg caaaattcgg tggcgcaaca
     8761 ggaaatttca atgcacattt tgttgcctat cccgatatcg actggatatt gtttgccgac
     8821 gaatttgtgt catcacgact cggacttgaa cgccagcgct acacaacgca aatagaacat
     8881 tacgataatc tggcagctgc attcgacgga ttgaaacgca tcaatacgat tttactcgat
     8941 ttaagtcgcg atatgtggac ttatatttcg atggattact tcaaacaacg catcaacaaa
     9001 aatgaagtag gctcatcggc aatgccacac aaagtgaatc ctatcgattt cgaaaatgct
     9061 gaaggtaatt tcggtatggc gaatgcactt ttcgaacacc tggcagctaa attgccgata
     9121 tctagattac agcgcgatct taccgattca actgtgctgc gcaatatcgg agttccaatg
     9181 gcacatacat taattgccat gaaatcagtg ttgaaaggat tgaagaaatt acaagtaaac
     9241 gagcaacgca ttcacgacga tctggataat aactgggcgg tgattgcaga agcaatacaa
     9301 accattcttc gccgcgaagg atatccgcaa ccttatgagg cattaaaaga actcacacgc
     9361 aacggacaag ccatcacaaa agatacctta caatcattta ttaatggtct cgatgtgagt
     9421 gatgatgtga aacaacagtt attgggtatc acgccattca attatacagg catacgcttt
     9481 ttctaattcc actaccaact gtcaaccatg tcgggcggaa aacttattga aggtgaagat
     9541 tattatatca atgccgaagg tctcttcgtg tttaccgctg catttttatt gaagagagga
     9601 ttttgttgcg gaaatggatg cgcacattgt ccatatccaa aatctgagga aaatacatat
     9661 ccggttgagg agaaaaaacc ttaggaaaat tggtatcagg tatcaagtat caggtatcag
     9721 gtatcaagta tcaggtatca ggtatcaagt atcaagtaaa aattcacgaa tcacgactca
     9781 cgattggtag tgagtagtgg gactagcccg gttggccctg taacttcgtt acaataaaat
     9841 aatttacatg acttctgaaa ttcactccct ctccctctgg gagaggaccg gggtgagggc
     9901 actcacccta ttttaatcgc ggagttggcg gaggtgcctg aacccgccac accattcacg
     9961 actcacgaat cacgattcac gattggtagt gagtagtggg actagcccgg ttggccctgt
    10021 aacttcgtta caataaaata atttacatga cttctgaaat tcactccctc tccctctggg
    10081 agaggaccgg ggtgagggca ctcaccctat tttaatcgcg gagttggcgg ag
//
LOCUS       DAOQHY010000122        10095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_3367_length_10095_cov_5.125000, whole genome shotgun sequence.
ACCESSION   DAOQHY010000122 DAOQHY010000000
VERSION     DAOQHY010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 10095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10095
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3367_length_10095_cov_5.125000"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /locus_tag="PLL28_10350"
     CDS             <1..1222
                     /locus_tag="PLL28_10350"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNF69768.1"
                     /translation="IPSYLMSLAVGDLAFKSVGKETGVYAEPSYVDACAEELSDMQAM
                     LESAEKLYGKYQWGRYDVIVLPPSFPFGGMENPRITFATPTIIAGDKSLVALVAHELA
                     HSWSGNLVTNATWNDFWLNEGFTVYFENRIMEALYGKDYADMLKVLEYESLVETVKEF
                     GSDSADTHLKLKLEGRDPDEGVSDIAYNKGAYFLRLLESKVGREKWDAFVNGYFTSNG
                     FKVMTTEAFIDYLNKNLIEPNKAAFADVDINAWIYGPGIPDNCPKITSTRFEQVDAEV
                     TKFKGGSAASSLATSGWSTHEWLRFIYQMPDDISPEKMSELDKAFKFTGAGNCEIADA
                     WYELAVRRHYTASYDAMDTFLNSVGRRKFLMPLYKAMMQTGQEALAKSIYAKARSGYH
                     YVAQNSLDDLVGKP"
     gene            1233..2255
                     /locus_tag="PLL28_10355"
     CDS             1233..2255
                     /locus_tag="PLL28_10355"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNF69769.1"
                     /translation="MTGTEQRKIIFIVGNSRSGTTMMRRVLGNHPDVYMLEELHFFEQ
                     LWSTNDKHRKMSREEAEQLASRLLFIQRDGYLTTMDLQKHANEAKAIIDSMQVDLFPH
                     EVLGAFLFHETRKNGRSIPCEKTPQDVFYIAEILELFPDARIVNMVRDPRGVLLSQKR
                     KWQRRAMGAGFMTKKEQRRLRINYHPITISKLWNSSIRAAAKYKGHAQVHNVNFEQLV
                     NASEAEVRSLCAFLGITFNANMLQIPQVGSSNEADKPDAFGIKTERAGNWEKGGLNKA
                     EIWFCQKICGENMHIYNYEVKPISANPFMILWYYLSFPVKIILAILFNLHRMKNIVEA
                     IKRRLS"
     gene            2306..3499
                     /locus_tag="PLL28_10360"
     CDS             2306..3499
                     /locus_tag="PLL28_10360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNF69770.1"
                     /translation="MSTGKKVLHITIHLPFPTNHGASLFNSYKLCTWLYRNHDLHFAC
                     FLKGDDVKYKDAFLEDTGIKNYYFEVLNVPRNGMNLIKSYLKGMTINMFRNYSADMHR
                     HIQSIAHEYDVIVAEHYEVMQYVPKDYKGKVVFRTHNAEYLIWSRFAEVETNPIKKLV
                     VSMEARRIKYWEFRYVKEADVVLGGTNDNEVHEPDPQKRALKFRDYLHIGDDSQITLP
                     IPDFEQLENSLVYVGTLTWEANVEGLLWFADGCWDILKERFPELKLYIIGKNPDQRLI
                     ELSKKYPDIIITGFVEDLEEYFMKCKVNIIPLRFGSGMKVKTINGLCRGIPMVCTTIG
                     AEGLKVTHDHDILISDTHAGFIQYVTELLNDNQKWNHIAHKSKATASKYYTWESLYKI
                     LEESI"
     gene            3660..4388
                     /locus_tag="PLL28_10365"
     CDS             3660..4388
                     /locus_tag="PLL28_10365"
                     /inference="COORDINATES: protein motif:HMM:NF015527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HNF69771.1"
                     /translation="MVIDHRYKFVFVELPLTATSAISKEIREQYGCESYLNKHATYDD
                     YLRKANSEMRKYTSIGSVRNPLDQTVSMYFKYKNDLDQRFSTGRKRPGKWLRKSLAKN
                     RDSVRYQYIIDNQASFEQYFMKFFKWPYSNWSIVHHKKMNHIIRFEHLVEDYRKVFTA
                     VGVPITRDLPQANKTPEKKADFWSYYESDAAKRRAKFVFGPFMRYWGYSFPASWDHVK
                     EPAHAQLVYNLLNIPRRIYWRLLR"
     gene            4461..5687
                     /locus_tag="PLL28_10370"
     CDS             4461..5687
                     /locus_tag="PLL28_10370"
                     /inference="COORDINATES: protein motif:HMM:NF015039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNF69772.1"
                     /translation="MRKLLLLLCLPTALYAQQPTKAEYRQSKSGYFQEVILTDINKAK
                     AEEAKDNRVFKIDASSVNAPKDPTKYTTVWHTNPVSQGNTGTCWCFSTTSFYESEVKR
                     NANIEVKLSEMYIVYWQYVERAKYFVANRGNMTFGEGSETNGTTWMIKQYGIVPYDSY
                     HGKTSTAGFYDHEPMFNEMNTYLQSIKTSNNWNEAEVVATIKSILNHYMGEPPTSVTY
                     NGKSYTPIEYTKNVLKINADEYVDFMSLMQKPFYAKAEYDVPDNWWNDASYYNLPINE
                     FMETLNTALKNGYSVSIGGDVSEPGWDSWNNIAVVPTWDIASNNIDDAARQYRFNTGA
                     TTDDHAMHLVGYTVQDGKTWYLFKDSGSGSRNCGAASPSFGYLYVSEDYVKLKMMTFT
                     VNKNAVKGALDKFAAK"
     gene            complement(5742..6428)
                     /locus_tag="PLL28_10375"
     CDS             complement(5742..6428)
                     /locus_tag="PLL28_10375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF69773.1"
                     /translation="MATIKILYVEDEANLGTIVSETLEQKGFDVRLIADGNLVLQACL
                     TFKPDIAVLDVMLPGVDGFTIGKNLRLQFPDLPIIFLTAKTQTKDVVEGFQSGGTDYM
                     RKPFSVEELIIRIQNQLRLRNVQQVASEIIRIGKFIYHPKKLLLENGENRWQLSSREA
                     EVLDVFSRYINRTIDRKTLLLEVWGDDSFFHSRNLDVYIRKLRDYFGSTNGVQIITLK
                     GKGYQFVVEN"
     gene            complement(6415..7875)
                     /locus_tag="PLL28_10380"
     CDS             complement(6415..7875)
                     /locus_tag="PLL28_10380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNF69774.1"
                     /translation="MSIKTTSSIGIRRIRALIALFCCAMLLLLMFTARWLQTEYNSEE
                     MDLHKELVQEFMSARDQVMDTVLTRSFIEPLLSNEGGFKIRHVSQDTITSGDSVTIIA
                     KSFDTDTMIRITSFCDTCPTLSEANLTNIDSNNALMRGVKLFITEVKGDKGGKEFFDR
                     HIELKDTALLQSIYQQNLADQGLNLEIKWVHDSSKFTFPPPMFEYDSHLFNEPFKAQV
                     DGYKSVILTTMVPQFLFAGLLITVVLVAFIFSYRNLRKQWQLAQLKDDLISNISHELK
                     TPVATMKVAIEAMQEMDPVEKKEKIRSYLNMASEEVTRLDTLVNKVMQSVIGDGAHEF
                     FHTTLIDVTGIANGLTNYLQSKHVDINVSIPETPCYVMGDAFHLRSALQNLLDNAAKY
                     GGTKVSLDVSQGADMVQISISDNGPGIPETYHQKIFEKFFRIPTGNVHEVKGYGLGLH
                     YVKQVTEAAGGSISVNNNSDTGCTFTLLIPRADGNH"
     gene            7996..8742
                     /locus_tag="PLL28_10385"
     CDS             7996..8742
                     /locus_tag="PLL28_10385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HNF69775.1"
                     /translation="MRKITLLLTMALSGLIAQAQTSGTIQYVSTRKLQINIQGDMPPP
                     PGLPSEIKLNTVLYFNTEASLYKNAENANDAMNVEQEAEGGGQIIMHMAPPENIMYCD
                     LTNKILTQQRDFMQRKFLIESPIDGSGWKLTGKQKMVLNYPCMEAIRVDSIDTVSAWY
                     TPVIPLSTGPLDYVGLPGMVMEVHVNNDMEVITATAVSKDVDASQIKKPKDGKKVTQE
                     EYDKIVEEKLKEMGVEPGPGGRVMIHIEND"
     gene            8753..>10095
                     /locus_tag="PLL28_10390"
     CDS             8753..>10095
                     /locus_tag="PLL28_10390"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNF69776.1"
                     /translation="MKKFAIYLVCIFLSVGLNAQVQNLSGHVFNSESKPVEYATVALL
                     QPQDSTLAFFAITDAEGAFSIKSVKAGTYRLQISFIGFDTYDTLLTTPLPNTNLGVYI
                     LQTAAIGLDAVQVTAERIPIQILGDTLQYNANSFKTAPDASAEELLKKLPGVEVDASG
                     NIKAQGEDVQQVLVDGKEFFSSDPTVATKNLPADAIEKVQVYDKTSDDADFTGVDDGQ
                     RSKTINLVLKDGKKSMWLGNAQAGYGTDDRYQTSAKLYRFTKTNQIAALGMLNNVNDF
                     GFSINDYIDFSGGIGNMMRSGGFQISSDNDVPVNFGQSVDGKITSGAAGLNYTYEPDE
                     DHRFNVSYLGNGYLRNLTQNTRTEQFSELGNFETIDSLNEETEKYFNRINFNIRTKPD
                     TLRSFIVTGNASMNMGDVTSSGFSANMFEDSLTNTLNNAAYSQNNNFSGTAAASY"
BASE COUNT         3149 a         2056 c         2094 g         2796 t
ORIGIN      
        1 cattccttcg tatttgatga gccttgccgt tggcgatctg gcatttaagt cggtaggtaa
       61 agaaaccggc gtgtatgcag aaccatccta cgtcgacgct tgtgccgaag aactcagcga
      121 tatgcaggct atgctcgaaa gcgcagaaaa actttatggc aaatatcaat ggggacgata
      181 tgatgttatc gtgcttccgc cttcattccc attcggaggg atggaaaatc cacgaatcac
      241 ttttgctaca ccaacaatta ttgccggcga taaaagcctt gttgcgctgg ttgcccacga
      301 attggcgcac agctggagcg gaaatctcgt cacaaatgct acttggaacg atttctggtt
      361 gaacgaaggc ttcactgttt atttcgaaaa ccgcatcatg gaagcacttt acggtaaaga
      421 ttatgccgat atgctgaaag tgctggaata tgaatcgctg gttgaaacag tgaaagaatt
      481 cggtagcgat tccgccgata cccaccttaa actcaaactc gaaggtcgcg atcctgatga
      541 aggcgtcagc gacatcgcct ataataaagg tgcttatttt cttcgtctgc ttgaaagtaa
      601 agttggtcgc gaaaaatggg atgccttcgt aaatggttat tttacttcca acgggtttaa
      661 agtaatgacc accgaggcat ttattgacta cctgaataaa aacctcatcg aaccaaataa
      721 agcggctttt gccgatgtag atatcaatgc atggatctat ggtcccggca taccggataa
      781 ttgtcctaag atcacctcaa ctcgatttga acaggtagat gctgaagtga caaaatttaa
      841 aggtggtagc gcagcttctt cattggctac aagtggatgg agcacacatg aatggctccg
      901 atttatttat caaatgccgg atgacatcag cccggaaaaa atgagtgaac tcgataaggc
      961 atttaaattt accggcgccg gaaattgtga gattgcagat gcatggtacg aacttgctgt
     1021 acgcagacat tatactgctt catacgatgc aatggatacc ttcctgaata gtgtaggaag
     1081 aaggaaattc ctcatgccat tatataaagc tatgatgcaa acagggcagg aggcactcgc
     1141 taaatctatt tatgccaaag cacgctccgg atatcattat gtagctcaaa attcactcga
     1201 cgatcttgta gggaagcctt aattattatt gtgtgaccgg aactgaacaa aggaaaataa
     1261 tttttattgt aggcaactca cgaagtggta ctacaatgat gcgtagggtg ctcggaaatc
     1321 atcctgacgt atatatgctt gaagaattgc atttcttcga gcagttatgg agtaccaacg
     1381 ataaacatcg caaaatgtcg cgcgaagaag ctgaacaatt ggcttcgcga ttactcttta
     1441 ttcaacgaga tggttacctc acaaccatgg atttgcaaaa gcatgccaat gaagcgaaag
     1501 caattattga tagcatgcag gtggacttat ttccacacga agttttaggc gcatttttat
     1561 ttcacgaaac gcgtaaaaac ggaaggtcta taccttgcga aaaaacaccg caagatgtat
     1621 tttatatcgc cgaaatatta gaattatttc ccgacgcccg catcgtaaat atggtgcgtg
     1681 atccgagagg tgtgctgctc agccaaaaaa gaaaatggca gaggagagct atgggtgcag
     1741 gatttatgac gaaaaaagaa caacgtcgac tgcgtataaa ttatcatcct atcaccattt
     1801 caaaattgtg gaattcgagt atccgcgcag cggcaaaata taaaggacac gcgcaagtac
     1861 ataatgtgaa cttcgagcag ctagttaatg cttctgaagc tgaagtgcgc agcttgtgtg
     1921 cgtttctcgg tataacattt aatgccaata tgctgcaaat tccacaagta ggttccagta
     1981 atgaagctga taagccggat gcttttggaa ttaaaacgga acgcgcagga aattgggaaa
     2041 aaggcggttt gaataaagca gaaatttggt tttgccagaa aatatgcggt gagaatatgc
     2101 atatttataa ttatgaggtg aagccgatta gcgcaaatcc attcatgatt ctttggtatt
     2161 atctctcatt ccccgtgaaa attattttgg caattttgtt taacctgcat cgcatgaaaa
     2221 atatcgttga agcaataaaa cgaagattgt cgtaaattgt tttcatcttc tattaacaat
     2281 atccctacca aaaatattta ttgttatgtc aaccggaaaa aaggtattac atatcaccat
     2341 acacttgccg tttccaacaa atcatggggc gagtcttttc aattcttaca aattatgcac
     2401 ctggttgtat cgtaatcatg acctgcattt tgcttgtttt ctaaaaggtg atgatgtgaa
     2461 atataaagat gcctttctgg aagatacagg tattaaaaat tattatttcg aagtgctgaa
     2521 tgtgcctcga aatggcatga atcttatcaa gagctatttg aaggggatga ccatcaatat
     2581 gttcagaaat tattcagccg acatgcacag acatattcaa tctattgcgc atgaatatga
     2641 tgtgatcgtt gctgaacatt atgaggtgat gcaatatgtt ccgaaagatt ataaaggaaa
     2701 agtggtcttt agaacacata atgcagaata cctgatttgg agccgcttcg ctgaagtaga
     2761 aaccaatcca attaaaaaat tggtggtaag tatggaagca cggcgtatta aatattggga
     2821 atttaggtat gtgaaagaag cggatgtcgt tctgggaggc accaacgaca atgaagtgca
     2881 cgagccggat ccgcaaaagc gagcactcaa attccgcgat tatttgcata tcggtgacga
     2941 ctcacagatc actctgccta tcccggattt cgaacaattg gaaaatagcc tggtatatgt
     3001 tggcacactc acatgggagg ctaatgttga aggcttgttg tggtttgctg atggttgctg
     3061 ggatattttg aaagaacgtt tcccggaatt gaaattatat attatcggaa aaaatccgga
     3121 tcagcgactg atcgaattga gcaaaaaata tcctgatatc atcattaccg gtttcgtgga
     3181 agatctggag gaatatttca tgaagtgcaa agtaaatatc ataccgcttc gtttcggctc
     3241 aggaatgaaa gtgaaaacta tcaatggact ttgtcgcggt atccctatgg tttgcactac
     3301 cattggtgct gaagggttga aagttacaca tgatcacgat attcttatct cagacacgca
     3361 tgcagggttt attcagtatg tcacagaatt gctgaacgac aatcaaaaat ggaatcatat
     3421 cgcacataaa tccaaagcaa ctgcatcgaa gtattacact tgggagtctt tatataagat
     3481 tttggaagag agtatctaga gtcaagctcg cctctggcgg gtatcaagcc caaaaaccac
     3541 tttgcgcctc tgcaactttg catctttgcg cgaacccaaa cacaaatcat attgtcttct
     3601 tttgagttgc acaagtaata tcatcattcc ccatccatca aatttcctaa ctttgcataa
     3661 tggtaatcga tcatcgatat aaatttgttt ttgttgaatt gccgttaaca gcaacttcgg
     3721 ccatcagcaa agagatacgt gaacaatacg gctgtgagtc gtatctcaac aagcatgcta
     3781 cctacgatga ttaccttagg aaggcgaatt ccgaaatgcg caaatacacc tccataggga
     3841 gtgtgcgcaa tccgctggat caaaccgtaa gcatgtattt caaatacaaa aacgatttag
     3901 atcaacgctt ttcaacagga agaaaacgcc cgggtaaatg gttgcgtaaa tcacttgcta
     3961 aaaacagaga ttccgttcgc taccaatata tcatcgataa tcaggcatcg tttgaacaat
     4021 acttcatgaa attcttcaaa tggccttaca gcaactggag tatcgttcac cacaaaaaga
     4081 tgaaccatat catcagattt gagcatctgg tagaagatta ccgcaaggta ttcacggcag
     4141 ttggcgttcc cattacccgc gatctgcccc aggcaaacaa aaccccggaa aaaaaggccg
     4201 atttctggag ctactacgaa agcgatgccg ccaaacgccg cgccaaattt gtgtttggac
     4261 catttatgcg ctattggggt tactcttttc ccgcctcttg ggatcatgtg aaagagccgg
     4321 ctcacgcgca attggtatac aatttgctga atatacctcg aaggatatac tggcgcctcc
     4381 tgcgttaaag tgtgttacat tccttgaatc cgttgcctct ccaggcgatt agacttatat
     4441 ttgacgtcag atcaaacaat atgagaaagt tacttttatt gctgtgcctt cctacagcac
     4501 tctacgcgca acagcctacg aaagctgaat atcgccaatc caaaagtggt tatttccagg
     4561 aagtaatcct caccgacatc aacaaggcaa aggctgagga agcaaaagat aatcgcgttt
     4621 ttaaaatcga tgcgtcatct gtcaatgcac ctaaagaccc aaccaagtat acaaccgttt
     4681 ggcataccaa tccggtaagt caggggaata ccggaacttg ttggtgcttt tctactactt
     4741 cattctatga gagcgaagtg aaaagaaatg cgaacattga agtaaagctg agcgaaatgt
     4801 atattgttta ctggcaatat gtggaacggg ccaaatattt tgtagccaac cgcggcaata
     4861 tgaccttcgg cgaaggaagt gaaacaaacg gaactacatg gatgatcaaa caatatggga
     4921 tcgttcctta cgacagttat cacggtaaaa caagtactgc cgggttttat gatcatgaac
     4981 ctatgtttaa tgaaatgaat acctatctgc aaagtattaa aacaagcaac aattggaacg
     5041 aagcagaagt ggtggcaaca attaaatcga ttctcaatca ctacatgggc gaaccgccaa
     5101 caagcgttac ttacaacggc aaatcctata cgccgattga atacaccaaa aatgtattga
     5161 aaattaacgc cgatgagtat gttgatttta tgagcctgat gcaaaaacca ttttatgcaa
     5221 aagcagaata tgatgtgccc gacaactggt ggaatgatgc ctcatattat aacttgccga
     5281 taaatgaatt catggagacc ttaaacactg ctttgaaaaa tggatatagt gtaagtattg
     5341 gtggcgatgt ttcagaaccc ggatgggata gctggaataa tatcgcagta gtgccaactt
     5401 gggatattgc atcaaataat atcgacgatg ctgcccgcca atatcgcttc aacaccggtg
     5461 ctacaaccga cgatcacgcc atgcatttag ttggttatac tgttcaggat ggcaaaacct
     5521 ggtatctctt taaagactca ggctccggat cacgcaattg cggcgccgca agccctagct
     5581 ttggatacct ttacgtgagc gaagattatg tgaaactgaa aatgatgacc ttcaccgtaa
     5641 ataaaaatgc cgtgaaaggt gcgctggata aatttgctgc taagtaaata agcaaggcat
     5701 attagaaagg gcaacaggaa tattgatttt ctgttgccct tttaattttc tactacaaac
     5761 tgatagcctt ttcccttcaa tgttataatt tgcacaccat tagtgctgcc gaaataatcg
     5821 cgaagttttc tgatgtaaac atcgagattt ctggaatgaa aaaaactgtc atcaccccac
     5881 acctccaata acaaagtctt tctgtctata gtgcgattga tatagcgact aaaaacatcc
     5941 agcacctctg cttcgcgact cgacaattgc catctgtttt cgccattttc aagcaatagc
     6001 tttttgggat gataaataaa tttccctatg cgaatgattt cagatgccac ttgctgaaca
     6061 ttgcgcaacc gtaattgatt ttgtattctg atgatcaatt cttcaacgct gaaaggcttg
     6121 cgcatgtagt ctgttccacc ggattgaaac ccttctacta cgtctttggt ttgtgttttc
     6181 gctgtcagaa aaataatggg taaatcggga aattgtaatc gcaggttttt acctattgta
     6241 aatccatcaa caccgggtag catcacatcc aacacagcaa tatcaggttt gaatgtcagg
     6301 catgcctgta gcaccagatt cccatcagca ataagacgca catcaaaacc tttttgctcc
     6361 agggtctccg aaacaatggt gccgaggttg gcttcatctt caacatataa tatcttaatg
     6421 gttgccatca gctctcggta ttaataaagt gaatgtgcat cctgtatcgc tattattatt
     6481 tactgaaata ctacctccag ctgcttcggt cacttgtttc acatagtgta agccaaggcc
     6541 ataacctttt acctcatgaa catttccggt gggaatgcgg aagaattttt caaatatctt
     6601 ttgatgatac gtttcaggaa tacccggacc attgtcgctg atggatatct gcaccatatc
     6661 agcaccctgt gacacatcaa gtgatacttt tgtgccgcca tacttcgctg cattatcaag
     6721 caggttttgt agcgcacttc gtaaatgaaa ggcatcaccc atcacatagc aaggtgtttc
     6781 aggtatgcta acgttgatat caacatgctt gctttgcaaa taattggtaa gtccattggc
     6841 aattccggtt acatcgatca aggtagtgtg aaaaaactcg tgggcaccat cgccaataac
     6901 ggactgcatt accttattta caagggtatc taagcgtgtc acttcctctg aggccatatt
     6961 taaatagctg cgtatctttt cctttttttc aaccggatcc atttcctgca tagcctctat
     7021 ggcaactttc atggtggcaa caggtgtttt caattcatga gaaatattac tgatcaggtc
     7081 gtcttttaat tgcgccaatt gccattgttt acgcaaattc cgataagaga aaataaaggc
     7141 aaccaaaact actgtaatca ataatcccgc aaaaagaaat tgaggcacca tggtggtcag
     7201 gattacactt ttatatccat caacctgagc tttaaacggt tcattaaata aatgactatc
     7261 gtactcaaac attggtggag gaaaagtgaa tttgctcgaa tcatgcaccc attttatttc
     7321 gagatttaat ccttggtcag ctaaattttg ttgataaata gattgaagaa gtgcggtgtc
     7381 ttttaattca atatgtctgt caaaaaattc ttttccaccc ttatcgcctt taacttcagt
     7441 aataaacaat ttcacacctc gcatcaaggc attattgctg tcgatatttg tcagattagc
     7501 ttctgaaaga gtagggcagg tatcgcaaaa tgaagtgatg cgaatcatag tatctgtgtc
     7561 aaatgacttt gctatgatgg taacggagtc tccggaggtg atagtgtcct gagagacatg
     7621 tcttatttta aatccgcctt cattgctcaa taacggttca ataaatgagc gtgtcagtac
     7681 agtatccatt acctgatcgc gtgccgacat gaattcctgc acgagttctt tgtgcagatc
     7741 catttcttca gaattatatt ccgtttgcag ccaccttgcc gtaaacatca gcaatagcaa
     7801 catcgcgcag caaaataatg ctataagcgc tcgaatgcga cgaataccta tcgaagatgt
     7861 tgtcttgatt gacatcatgc aaaattagga ctttcagaca ctttaacact tccgcttaac
     7921 cttaattaac ctaaattgct ttcagttaac cccacccttc cacacagcct tgtagctttg
     7981 catcaaaaca ataacatgcg aaagatcaca cttttactca caatggcatt atcaggtttg
     8041 atagcccagg ctcaaacttc cggtaccatc caatacgttt caacccgaaa attgcagata
     8101 aatattcaag gtgatatgcc acctcctccg ggattgccaa gtgaaattaa actaaataca
     8161 gtcttgtatt ttaataccga agcttcactt tataaaaatg ctgaaaatgc aaacgatgca
     8221 atgaatgtgg agcaggaggc agaaggcggc ggacaaatta tcatgcatat ggcccctcct
     8281 gaaaatatca tgtattgtga tctcacaaat aaaatactta cacagcaacg cgatttcatg
     8341 cagcgcaaat ttttaattga atcaccaata gatggttcag gttggaagct gacagggaag
     8401 caaaaaatgg tgcttaacta tccttgcatg gaagcaattc gcgttgatag catagacacg
     8461 gtaagtgctt ggtatacacc ggtgattcca ttgtcgacag gaccacttga ttatgtcgga
     8521 cttcccggaa tggtaatgga agtgcatgtg aataatgata tggaagtgat taccgcaact
     8581 gccgtttcaa aagatgtaga tgcttcacaa atcaagaaac ctaaagatgg aaagaaagtt
     8641 actcaagaag aatatgataa aattgttgaa gagaaattaa aggaaatggg cgtagaaccg
     8701 ggtccaggag gaagagtaat gattcatatt gaaaacgatt aatattttaa tgatgaagaa
     8761 atttgcaatt tatcttgttt gtatcttttt gtctgttgga ttgaacgcac aagtgcaaaa
     8821 tctcagtggg catgttttca acagcgaaag caagcctgtt gaatatgcta ccgtcgcact
     8881 tttgcaacca caggattcta cactcgcttt ttttgcgatc acagatgcag aaggggcatt
     8941 ttctatcaag agtgtaaagg ccggaactta cagattgcaa atttctttca ttggcttcga
     9001 tacctacgat accttgctta caacgccact gccgaatacc aacttaggcg tgtatatttt
     9061 gcaaacagct gccatcggac ttgatgcggt acaagttact gcggaacgaa ttcctattca
     9121 gatattagga gataccctgc agtataatgc aaactccttt aaaacggcac ctgatgcctc
     9181 ggcagaagaa ttattgaaaa aacttcccgg tgttgaagtg gatgcttccg gaaacattaa
     9241 agcccaaggt gaagatgtgc agcaagtgct ggtagacggt aaagaatttt tcagcagcga
     9301 tccgactgtg gccacaaaaa atttaccagc tgatgccatt gaaaaagtac aggtgtacga
     9361 taaaacatcc gacgatgcag attttacagg tgtcgatgac ggtcaacgca gcaaaaccat
     9421 caacctcgtg ctgaaagatg gtaaaaaaag catgtggctc ggcaatgcgc aggcaggtta
     9481 tggcaccgac gatcgttatc agacttctgc caaattatat cgcttcacca aaacaaatca
     9541 aatcgctgct ttaggtatgc tcaataatgt caatgacttt ggattcagca tcaacgatta
     9601 tatcgacttc agtggaggca tagggaatat gatgcgcagc ggtggatttc aaatatcatc
     9661 ggataatgat gtccctgtta atttcggtca atctgttgat ggaaaaatca caagcggagc
     9721 tgcaggattg aattatacct acgagcctga tgaagaccac cgcttcaatg tcagctatct
     9781 gggaaatggc tatctgagaa acttaacgca aaataccagg acagaacaat tttcagaact
     9841 tggaaatttt gaaaccatcg actcactcaa tgaagaaact gaaaaatatt ttaatcgcat
     9901 caattttaat atcagaacaa aacctgatac acttcgctca tttattgtaa caggtaatgc
     9961 ctccatgaat atgggcgatg ttactagctc gggattcagc gcaaatatgt ttgaggatag
    10021 tcttaccaat acactcaaca atgcagcata ttcccaaaac aacaatttct caggtactgc
    10081 tgcggcaagt tatat
//
LOCUS       DAOQHY010000123         9953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_3447_length_9953_cov_5.230148, whole genome shotgun sequence.
ACCESSION   DAOQHY010000123 DAOQHY010000000
VERSION     DAOQHY010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9953
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3447_length_9953_cov_5.230148"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PLL28_10395"
     CDS             complement(<1..496)
                     /locus_tag="PLL28_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF69777.1"
                     /translation="MANTNYNYSEEHIRTLDWIEHMRMRPGMYIGKLGDGSSPDDGIY
                     ILVKEVIDNAIDEYMMGYGKRIDVTIDKHQVTVRDFGRGIPLGKVIECVSVMNTGAKY
                     DTAAFQKSVGLNGVGTKAVNALSQYFRVEAYREGKTKVAEFEKGLIKKDSAISKTEQK
                     DGTYV"
     gene            complement(581..1930)
                     /locus_tag="PLL28_10400"
     CDS             complement(581..1930)
                     /locus_tag="PLL28_10400"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNF69778.1"
                     /translation="MAAKRRNKPQEEKKIFILDTSVILHDHNSVNKFEEHDVVIPITV
                     LEELDNFKKGNETINFEAREFIRFLDNKSKESTLQEWRPINGKSTGRMIVMMYEDHGV
                     DAEVIFGEEKPDHKILNTALVVKHRYPEQTVVLVSKDINLRIKAKALGLLAEDYKNDK
                     IQDPEFLKEPIETIEGIDHGTIDKIYSDGFIGFKKVLKHEPYSNQCFILKNGSSSVLA
                     SYNSDDKVFQRINKASAYGIKPRNAEQVFALSMLLNPNVKLIALQGQSGTGKTLLALA
                     SALEQRRQYKQIFLARPIIPLNNKDIGYLPGDIKSKLNPYMEPLWDNLKMIQNQFEQA
                     DSEYKAIAQMIEQEKLVIQPLAYIRGRSISNIYFIIDEAQNLTPLEVKTIITRAGENT
                     KIVFTGDINQIDTPYLDTHSNGLSYLIDKIKNHPMCSVITLQRGERSELANLANDLL"
     gene            complement(2027..3670)
                     /locus_tag="PLL28_10405"
     CDS             complement(2027..3670)
                     /locus_tag="PLL28_10405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNF69779.1"
                     /translation="MSLSAISRLNDIRSAVEQHWDIIVIGGGITGAGIALEASCRGMK
                     VLLLEQYDFASGTSSKSTKLIHGGLRYLKQLEFGLVRTVGRERAVIHKLAPHLVIPTR
                     MVLPITEGGTLGKYSTSMALFVYDRLAGVKKHERYQMLTPQALHQMEPLLRSDNVKGG
                     AVYIEYRTDDARLTMAVLKSAHEKGAACFNYLKVTGFSQSNNHINGVEALDLLNNTKH
                     SFNTKLVINAAGPWVDDVRTLEELPHGKRLHLTKGIHIVIPASKFPLEHAIYFDVATD
                     DRMIFAIPRGEVVYVGTTDTNYRDSKEHILIDNADIDYLLSAITEMFPSIHLTRRDVE
                     SGWAGLRPLIHEEGKSPSELSRKDEIFVSKQGMISIAGGKLTGYRKMAEKAVRKAVDL
                     LRKRDGLQFPKANTAQLLLQGSHFSTSIDKYIALRAGEAKQAGISAEQVSRLVFTYGT
                     DAAEILDDVFAHIQRLQDPQQALLFAEVRYAIQNEMVTGVSDFLIRRTGSLYFNKRFA
                     TENYRLIAEIIADELQMDASDAKLQLQWFEAVLEELKFS"
     assembly_gap    4037..4136
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4218..5295
                     /gene="prfB"
                     /locus_tag="PLL28_10410"
     CDS             join(4218..4277,4279..5295)
                     /gene="prfB"
                     /locus_tag="PLL28_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217450.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNF69780.1"
                     /translation="MTQEHYNELMDKLLVLRRFLNYDDKLIQVKEDEQITHQPGFWDD
                     PKNAEVVLKRIKINNVWINSYNEAQQAVDDLGVLYEFSQMGEATEEEVDAQFAKTKER
                     LEELEFTSTLTEKEDALPAILTINAGAGGTESCDWASILYRMYMMWANKKGFTVNELD
                     FQAGDVAGIKSASLEIDGEFAYGLLKGENGVHRLVRISPFDANAKRHTSFASVFVSPM
                     IDDTIEINISPSDLTWDTFRSGGAGGQNVNKVETAVRVTHLPTGIVVSCQVNRTQGQN
                     RELAMQMLRSKLYDLEMQKRQEVKDKIEGTKKKIEWGSQIRNYVLHPYKLVKDARTGH
                     ETGNAQAVLDGDLDDFIKAYLLAE"
     gene            5365..7554
                     /locus_tag="PLL28_10415"
     CDS             5365..7554
                     /locus_tag="PLL28_10415"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF69781.1"
                     /translation="MSPNRLQLVVISALSFSLCFATKASAQISCTFAVVDAADGNPLP
                     GAVIEIPAITYVNATDDNGYLHVTQLPSGKFSLHCSMISYQPLDTIIQLQHGAGDTVV
                     FSMYSALEHLEEVNISSTRTNARIEDDPTRIEVLGLEEMGEESTLVPTGIGSILGDLA
                     IITIQRTSVSTGNDEIRMAGMDPRYTQMLRDGLPLYSGASGGLGALDIPPLDLQQVEV
                     IKGSNSTLYGGGAIAGLINFVSRKPTKDLVIDGVLNMTSLLGSHASVFSGKQFEQLGY
                     TCFAAATINQPVNVKSDNYSIIPQEQQFIVHPRIFYTLHESTKLDVGFQWNHDKLNSG
                     FMDRAYHLQEGGYYAYNIADRFVEDITITHKWNAKSTLTFKSALSTFSRTQDVHTTDT
                     LATPFFSLQEMNGYTELSNAFHWGSHTFLAGYNFLFEQFDPGNEGLQLSKTAQYTQGI
                     FLQDDWHISEKFTCEIGLRGDVHSVYGSAVLPRIALFYKPAKDWSCRISGGSGYKTPN
                     ALDLSEPAKQLVTPVTSLHLERSEGVNADVNFHRLLNDHISLECNQALYYIMLQHPYA
                     LQTDDPENYVVVNDAGDVTSIGSDTYIRLEIDEIECYLGYNHTIAQKQQAINIYLPFY
                     PHDKLSGVLAYDNDTWRMGIEASYQANQYLSDQTKAPDYLFMAAMISRSLGHFRLTLN
                     GENLTNAIQQNLTDIPIVSGSLLEPTFSDIWAPIEGITINASLKYTF"
     gene            complement(7555..8514)
                     /locus_tag="PLL28_10420"
     CDS             complement(7555..8514)
                     /locus_tag="PLL28_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNF69782.1"
                     /translation="MAEKVTISGFSFVRNADRLFYPVKESILSILPLVDEFVIAVGDN
                     APGDGTIALIESIQSPKIKILHTVWDTETYPKATIYAQQTNVAMKACTGDWLFYIQGD
                     EVVHEKDLDAIRKQCEKYADDPEVEGLLFDYYHFWADYAHINRSHSAYAQEIRMVKNR
                     KDIFSYGDAQGFRRIPGFDMRNFRQKENVFKLKVAPAQAHIYHYGWVRPPDFMMRKKQ
                     FANTLHHGQAETDANYKAAMFDYGPVGRKFPFKDTHPAVMAERVKSFDWGHLLNNGKS
                     QKHYNRPPLKHEKWHIRVWSWFEIFVFRKQIFTAKKFTLIRRD"
     gene            complement(8536..9411)
                     /locus_tag="PLL28_10425"
     CDS             complement(8536..9411)
                     /locus_tag="PLL28_10425"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNF69783.1"
                     /translation="MEHREAEIQALIALLDDSDPEVFDHVAARLLSYGPMVIDRLEDA
                     YTTIPNPVMQERIEDIIHQIQFAGVLADMRNWASHGHNDLMRGLLIITRHQYPDLDED
                     AILANVARIQKDIWIGINTYLSPLEQMNVINQSLFSQYQFAGLQNNDDELRYAYINNM
                     LDAFKGNHFSVGLLYLILCQQLDMPVYGVLLSTHFILARMKEDIVNFEGDNKDDILFY
                     INPYNKGLAFSEREIKGYLSKLEIPSDEKYFKPASNKAVLAEYIRHLISLIKKPQDQY
                     KIEDLEALLACLESE"
     gene            complement(9417..>9953)
                     /locus_tag="PLL28_10430"
     CDS             complement(9417..>9953)
                     /locus_tag="PLL28_10430"
                     /inference="COORDINATES: protein motif:HMM:NF024761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HNF69784.1"
                     /translation="MGKFGPMVVMGELLDPKEAKAKGDGKKPKFAGLLKHQNMAEITL
                     EDALKLFELPKTIGTYREDDVVVGVGRFGPYVRNMKKFYSIPKGTEAAAITLEEAIQL
                     INDKDLKDQNKLIHDFPEHEIQVLNGLYGPYIKKGKDNYKIPKDRVAKDLTLEQILEI
                     VETTEPSGKGKRKFAKKK"
BASE COUNT         2747 a         2208 c         2006 g         2892 t
ORIGIN      
        1 tcacataggt gccatccttt tgttctgttt tagaaatggc gctgtctttt ttgatcaagc
       61 ctttctcgaa ttcagcaact ttggttttgc cttcgcgata tgcttcaaca cgaaaatact
      121 gacttagtgc attcaccgct ttggtaccta caccgttcaa accaacagat ttctggaatg
      181 ctgctgtgtc atatttggca ccggtattca ttacgctcac gcattctatc acttttccca
      241 gtgggatacc acgaccaaaa tcgcgaacgg taacctgatg cttgtcgata gttacatcaa
      301 tgcgctttcc ataacccatc atatactcat caatagcatt atcgatcacc tcttttacaa
      361 gaatgtaaat accatcatca ggagatgaac catcgcccag tttcccgata tacataccgg
      421 gacgcatgcg catatgctcg atccagtcga gcgttctgat atgttcttcc gaataattat
      481 agttcgtatt agccatgccg cttcagtttt cctttcttac acaatattag gtcaatcgtt
      541 gaaatgtgct ctccattttc ctgtgcataa ctgtcgtgac ttacaacagg tcgtttgcca
      601 gattcgccag ttcagaccgc tctccgcgtt ggagtgtgat cacagaacac atcggatgat
      661 tttttatttt atcgattaga taactcaatc cattactatg cgtatcgaga tatggtgtat
      721 ctatctgatt gatatcgccg gtaaagacaa tctttgtatt ctcccctgcc cttgtgataa
      781 tagttttcac ttccaggggt gtgaggttct gcgcttcatc aatgataaaa taaatgtttg
      841 aaatactgcg acctcgtata taggcaagcg gttgtatcac caatttttcc tgctctatca
      901 tttgcgcaat tgctttatac tcactatcgg cctgctcaaa ctgattttgg atcattttca
      961 gattatccca aagtggctcc atatatggat tcaacttcga tttaatatca cccggtaaat
     1021 atccgatgtc tttattattc aatggaataa tcggcctggc aagaaatatt tgcttgtatt
     1081 gtctgcgctg ctccaaagca ctggccaatg ccaacaaggt ttttcccgtt ccactttgtc
     1141 cttgtaaggc aataagttta acattcggat tgagcaacat actcagcgca aatacctgct
     1201 cagcattgcg cggtttaatg ccatatgctg aagctttatt gatacgttga aaaaccttat
     1261 cgtcggaatt ataactcgca agcacactgc tgctgccatt cttcagaata aagcactgat
     1321 tggaataagg ttcatgtttt aaaacctttt tgaatccgat aaatccatcg ctatagattt
     1381 tatctattgt tccgtgatcg attccttcga tagtttcaat tggttctttc aggaattcag
     1441 gatcttgaat tttatcattt ttgtaatcct cggcaagcaa acctaaggcc ttagctttta
     1501 tgcgcagatt aatatcctta ctcaccaata caacggtttg ctccggataa cgatgcttga
     1561 caaccaaggc agtattgagg attttatgat caggtttttc ttcgccaaat atcacttcag
     1621 catcaacgcc atgatcttca tacatcatta caatcattct gcccgtcgat ttgccgttga
     1681 tcggacgcca ttcctgtaaa gtcgactcct tcgatttatt atcgaggaag cggataaatt
     1741 ctctggcttc aaaattgatg gtttcattac cttttttgaa gttatccaat tcctccaaca
     1801 ccgtgatagg aatcactaca tcatgttctt cgaatttatt tactgaattg tgatcgtgca
     1861 ggatgactga agtatcgaga ataaatatct ttttttcttc ctgcggttta ttacgcctct
     1921 ttgcagccat aaattagttt ctaagccaat gttagtagct atacagcaaa ttccaaagac
     1981 agttatacca ctatcctgtt gataattgta ccatgcagcg tacaggtcag ctaaatttaa
     2041 gctcttccaa aacagcctca aaccattgca gttgcagctt tgcgtctgat gcatccattt
     2101 gaagttcatc ggctatgatc tcagcaatta gtcgataatt ctctgttgca aagcgcttgt
     2161 taaaatataa actgccggtg cgtcttatca ggaaatcact gacgccggtt accatttcat
     2221 tttgtatcgc atatcgcacc tccgcaaata ataaagcttg ctgtggatct tgcaatcgtt
     2281 gaatatgggc aaaaacatca tccagaatct cagcggcatc ggtaccatag gtaaacacca
     2341 gcctgcttac ttgttccgct gaaatacctg cctgttttgc ctctccggcg cgaagtgcga
     2401 tgtatttatc aatgcttgtc gagaaatgcg aaccctgcaa taatagttgt gcagtgtttg
     2461 ctttcgggaa ctgcaatcca tctcttttcc tgagcaaatc aactgccttc ctaacggcct
     2521 tctcagccat cttgcgatat ccggtaagtt tgcctccggc gatggagatc ataccttgct
     2581 tggaaacaaa tatctcatcc ttgcgtgata gctctgatgg tgattttcct tcttcatgta
     2641 tcaatggacg caatcccgcc caacccgact ctacatctct tcgcgttaag tgaatactcg
     2701 gaaacatttc ggtaattgca cttaacaaat aatcaatatc agcattgtcg ataagaatat
     2761 gctccttgct gtcgcggtaa ttggtatcag tggtgccgac atagaccact tctcctcgtg
     2821 gaatagcaaa aatcattctg tcgtctgttg ccacatcgaa ataaattgca tgttccaatg
     2881 ggaatttact tgcagggata acgatatgta tgccttttgt aagatgcaat ctcttaccat
     2941 gaggcaactc ttccaatgtg cgcacatcat caacccacgg gccggcagca tttatcacta
     3001 atttggtgtt aaagctatgc ttggtattat ttaaaaggtc cagcgcttct acgccattaa
     3061 tatgattatt actttgcgaa aagcctgtca ctttaaggta attaaaacat gctgcacctt
     3121 tttcatgagc agattttagc accgccatcg ttaatcgggc atcgtcggtg cgatattcaa
     3181 tatacacggc accacctttt acattgtcgg aacgcaacag cggttccatt tgatgtaagg
     3241 cctgtggtgt aagcatttga tagcgctcat gcttttttac tcctgccagg cgatcgtaaa
     3301 caaacaatgc catggaagtt gaatactttc ctaatgtacc gccttcagtg atcggtaaaa
     3361 ccattcgcgt tggaataact agatgaggtg ccagcttatg aattactgct cgctcacgtc
     3421 caacagttcg cactaatcca aactcaagtt gtttcaaata gcgcaatccg ccatggatca
     3481 gctttgtaga ttttgatgat gtgccacttg caaaatcata ctgttcaagc aaaagcacct
     3541 tcatgcctct acacgaggcc tcaagtgcaa ttccagctcc tgttatgcct cctcctatta
     3601 caataatatc ccaatgctgt tcaacagcac tgcgaatatc atttaaacgc gaaattgcag
     3661 ataaggacac ggggtaaaat taaggagaaa ggagaaagga gagaggagat aggagagagg
     3721 agagaggggg aattggctga ttagttaatg ggattgtatt ggaatggaga tttttgatgg
     3781 agttagcaag tttgagggtt agcaagttag caggtggggt gggtttcgcg caaaaacagc
     3841 gaagaagcta agggttagca agttagcagg tggggtgggt ttcgcgcaaa aacagcgaag
     3901 aagctaaggg ttagcaagtt agcaggtggg gtggatttcg cgcaaagaca gcgaagaagc
     3961 taaggcgcaa ttaactcacc cagtttgagg gttagcaagt tagcaggtgg ggtgggtttc
     4021 gcgcaaaaac agcgaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaccc
     4141 actgacccac tcacccactc acccctctcc tctctcctct ctcctctctc ctctctcctt
     4201 tttcctatct ttgccccatg acacaagaac attataacga gctaatggat aaattgcttg
     4261 ttttgaggag gtttctttaa ctacgatgat aagctcatcc aggtaaaaga agacgaacag
     4321 atcactcacc aaccgggttt ctgggatgac ccgaaaaatg cggaagtggt cctcaagcga
     4381 atcaagatca acaatgtatg gatcaacagt tataacgagg cgcaacaagc agttgatgac
     4441 cttggtgtgc tgtatgaatt ctcgcaaatg ggcgaggcta ccgaagaaga agtagacgct
     4501 cagtttgcaa aaacgaaaga gcggttggag gaattagagt ttacatccac cctcaccgaa
     4561 aaagaagatg ccttacccgc tattctcacc attaacgcag gtgccggtgg aaccgaaagc
     4621 tgcgattggg cgagtatact atatcgcatg tacatgatgt gggccaacaa aaagggcttc
     4681 acagtaaatg aattggattt tcaggccggt gatgttgcag gaattaaatc agcgtcttta
     4741 gaaatagatg gtgaatttgc ctacggattg ctgaaggggg aaaatggtgt gcatcgttta
     4801 gtgcgcatct caccattcga tgccaatgcg aagcgacata cttcatttgc ttccgtgttt
     4861 gtttcgccaa tgatcgatga cacgatagag attaatattt ctccgagcga ccttacatgg
     4921 gataccttcc gaagtggtgg tgcaggtgga caaaacgtaa acaaggtgga aactgctgtt
     4981 cgcgttacac atcttccaac agggattgta gtttcttgcc aggttaatcg tacgcaagga
     5041 caaaaccgcg aactcgccat gcagatgttg cgcagtaagt tgtatgatct cgaaatgcaa
     5101 aagcgtcagg aggtgaaaga taaaatcgaa ggcaccaaga aaaagatcga atggggaagc
     5161 cagatccgaa actatgtatt gcacccgtat aaactcgtga aagatgcccg caccggtcac
     5221 gagacaggca atgcacaagc cgttctggat ggcgacctgg atgattttat caaggcttac
     5281 ctgctggcag aataatttca gtttaacaag ctttaatagt gacatgtaca tcttcgtgtt
     5341 acctttattg cataattact tgcattgtcg ccaaatcgct tacagctggt tgtaatttca
     5401 gcactgagtt ttagtttgtg tttcgccacg aaagcatccg cacagatcag ctgcaccttt
     5461 gcagtagtag atgcggccga cggaaatccg ctgcccggtg ctgttattga aatccctgcc
     5521 attacttatg tcaatgcgac agacgataat ggttatttgc atgttaccca actgccttcg
     5581 ggaaagttca gtctgcattg ctcaatgata tcttatcaac ctcttgatac gataatacaa
     5641 cttcagcatg gagccggtga tacagtcgtt ttttcgatgt atagcgctct tgaacatctg
     5701 gaagaagtaa acatcagcag cacgagaaca aatgcacgca tcgaagatga tcctactcgt
     5761 attgaagtac tcggactcga agaaatgggt gaagagagca cgctggtgcc aacaggtata
     5821 ggtagcattt tgggagatct ggccattatt accatacagc gaacttccgt ttccacaggt
     5881 aatgatgaaa ttcgcatggc agggatggat ccacgataca cccagatgct gcgcgatggg
     5941 cttccactgt attcaggagc ctccggtgga ctcggcgcac tcgatatccc cccactcgat
     6001 cttcagcagg tggaagtgat aaaaggaagc aattcaacat tgtatggcgg cggagcaatt
     6061 gctggtttga taaattttgt atccagaaaa ccaacaaaag atctggtgat agatggtgta
     6121 ttaaatatga catctcttct ggggtcgcat gccagtgttt tttcaggtaa acaattcgaa
     6181 caattaggat acacctgctt tgcagcagct actatcaatc agccggtaaa tgtgaagtct
     6241 gacaactatt ccatcatccc acaagaacaa cagttcatcg tgcatccacg tattttttat
     6301 acattgcatg aatcaactaa actggatgtt ggatttcaat ggaatcacga taaactcaat
     6361 tctggtttca tggacagagc ctaccatctg caggaaggtg gctattatgc atataatatt
     6421 gccgaccgtt tcgtggaaga tatcacgatc acacacaagt ggaatgcaaa aagcaccttg
     6481 acattcaaat cagcgctaag cacattttcc agaacacagg atgtgcatac tacagataca
     6541 ctggcaaccc catttttcag tctacaggaa atgaatggct acacagaatt atcaaatgct
     6601 tttcattggg gatcacatac attccttgct ggatacaatt tcctgttcga acagttcgac
     6661 ccagggaatg aaggattgca attatcaaag acagcacaat acacacaagg cattttctta
     6721 caagacgact ggcatataag tgaaaaattc acatgcgaga tcggcctgag gggcgacgtg
     6781 catagtgtgt atgggtcagc tgttcttccc cgcatcgccc tgttttataa gcctgctaaa
     6841 gattggagtt gcaggatcag tggtggcagt ggatacaaaa caccaaatgc gctcgatctt
     6901 tctgaaccgg ccaaacaact tgttacaccg gttacatccc ttcatttaga gcgttctgaa
     6961 ggtgtgaatg ctgatgtaaa tttccatcgc ctgctcaatg accatatatc tctagaatgc
     7021 aatcaagcat tatattatat catgttacag catccatatg cactacagac agatgaccct
     7081 gaaaattatg tggtggtgaa tgatgccggt gatgtcacaa gtatcggatc tgacacatat
     7141 atcagattgg agatcgatga aatcgaatgt tatcttggat ataatcacac catcgcgcaa
     7201 aaacaacaag cgataaatat ctatttaccc ttttatcccc atgataaatt atccggcgta
     7261 ttagcctacg ataatgatac ttggcgcatg ggtatagaag ccagctatca agcgaatcaa
     7321 tatctttcag atcaaaccaa ggcacctgat tatctattta tggctgcaat gatctcaaga
     7381 tcgctcgggc atttccgttt gactttgaat ggtgaaaatc tcaccaatgc catccagcaa
     7441 aatttaacgg atattcctat cgttagcggt tcgcttttag aacctacttt ctctgatatt
     7501 tgggcaccaa ttgaaggcat cacgataaat gcctcattga aatatacatt ctgatcaatc
     7561 tctgcggatc aaggtgaatt tttttgcggt gaatatctgc ttgcgaaata caaatatttc
     7621 aaaccagctc cacactcgga tatgccattt ttcatgcttt aatggtggcc gattatagtg
     7681 tttttgcgac ttcccgttat ttagcaaatg cccccagtca aaggatttca cacgttcggc
     7741 cattacggca ggatgcgtgt ccttaaaggg aaatttccta ccaaccgggc cgtaatcaaa
     7801 catcgccgct ttgtagtttg catctgtttc cgcttgcccg tgatgcagtg tattggcaaa
     7861 ctgcttcttg cgcatcataa aatccggtgg tcgcacccag ccgtagtgat aaatgtgcgc
     7921 ttgtgccgga gccactttta atttaaatac gttttccttc tgtctgaaat tgcgcatatc
     7981 aaatcccggt atcctgcgaa atccctgtgc atcaccatag gaaaaaatat ccttgcgatt
     8041 cttcaccatg cgtatttcct gtgcatacgc gctgtgcgat ctgttgatat gcgcataatc
     8101 agcccagaaa tgataatagt cgaacaataa cccttccacc tccggatcat cagcatattt
     8161 ctcgcactgc tttctgatgg catccaggtc tttttcatgc acaacttcat caccttggat
     8221 ataaaacaac cagtcaccgg tacacgcttt cattgccaca ttggtctgct gggcataaat
     8281 ggttgccttg ggatatgttt cggtatccca aacggtatgc aatatcttga tcttgggcga
     8341 ctgtatactt tcgatcaatg caatcgtgcc atcaccgggc gcgttgtcac caacagcaat
     8401 cacaaattca tcaacgagag gtaaaatgga aagtatagat tccttcaccg gataaaacaa
     8461 tctatcggca ttgcgcacaa agctaaatcc gctgatggtt accttttctg ccatgtgtaa
     8521 aatgttgcgc gtgaattatt ccgattccag acatgccaac aatgcctcga ggtcttcgat
     8581 cttatattgg tcctgtggtt ttttgatcaa ggaaatcaaa tgcctgatat actctgccaa
     8641 cacagcctta ttggatgccg gcttgaaata tttttcgtca gaaggtatct ccaacttact
     8701 gagatatccc tttatctcgc gctcactgaa tgctagtcct ttgttgtagg gattgatata
     8761 aaataaaata tcgtctttat tatcaccttc aaaattgaca atatcctctt tcatccgagc
     8821 aaggataaaa tgggtgctca acaatacacc ataaacaggc atatcgagtt gctgacatag
     8881 aataagataa agcaagccta cagaaaagtg attcccttta aatgcatcca gcatattgtt
     8941 gatatatgca tatcgaagtt catcatcatt attttgcaac cccgcgaatt ggtattgtga
     9001 aaacaaagat tgattgatca cattcatttg ttctaaaggc gataaatagg tattaatacc
     9061 tatccatata tccttttgta tacgtgccac atttgcaagg atggcatctt catccagatc
     9121 gggatattgg tgacgggtaa tgatgagcaa ccctcgcatc agatcgttgt gaccatgaga
     9181 agcccaattg cgcatatcgg ctaatactcc tgcaaattga atttgatgaa taatatcctc
     9241 tatcctttcc tgcataacag gattgggaat ggtagtatac gcgtcttcaa gcctatcgat
     9301 aaccattggc ccataagata ataatcgcgc agctacatgg tcgaatactt cgggatcact
     9361 atcatccagc aatgcgatca gcgcttgtat ttctgcttca cgatgctcca tggaggctat
     9421 ttctttttag caaacttgcg tttaccctta ccggaaggtt ctgttgtttc aacgatctcg
     9481 aggatctgtt ctaatgtcag atcttttgca accctatctt tcggtatctt ataattatcc
     9541 ttgccttttt tgatgtaggg accatataat ccattcaata cctgaatctc atgttcagga
     9601 aaatcatgaa tgagtttatt ttgatccttc aggtccttat cgttaatcaa ctgaattgct
     9661 tcttcaagtg taatggcagc agcttctgtc cctttaggaa tagaatagaa ctttttcatg
     9721 ttgcgcacat atggaccgaa gcgccccaca ccaacaacca catcatcctc gcgatatgta
     9781 ccgatagttt taggtagttc aaataatttc aaggcatctt caagtgtaat ttcagccata
     9841 ttttgatgct tgagcaatcc tgcgaatttt ggttttttac catcgccctt tgcctttgcc
     9901 tctttgggat cgagcaattc acccatcact accataggtc cgaatttacc cat
//
LOCUS       DAOQHY010000124         9787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_3521_length_9787_cov_6.581073, whole genome shotgun sequence.
ACCESSION   DAOQHY010000124 DAOQHY010000000
VERSION     DAOQHY010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9787
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3521_length_9787_cov_6.581073"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..1640
                     /locus_tag="PLL28_10435"
     CDS             201..1640
                     /locus_tag="PLL28_10435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HNF69785.1"
                     /translation="MSSAVRNLEPKAIWNFFEDINAIPRPSKHEERISAYMVAFGEQL
                     GLRTVKDAVGNVIIFKPATTGMESKPTVILQSHLDMVHQKNSDTQFDFNTEGIRSRID
                     GDWVKAEGTTLGADNGMGVAAIMSLLASKDIPHPALEALFTIDEETGMTGAKELQKGL
                     LQGKILLNLDTEDDDEFSIGCAGGIDTNTSYDYTQEAPPTGAMAFRISVSGLKGGHSG
                     VDIHLERGNANKIMNRLMFDGDAFGLQIASIDGGSLRNAIPRESFATVVVNNAKYADE
                     VRARAAAICHEYHLQDEGLKIDIQSTEMPGMVMSFDDSRKLILAIQSAHNGVYTMSSA
                     ISGLVETSSSLARVIVKDGKFITQSLQRSSVESAKMDVAYTVAAPFRLMGCSVEHGGA
                     YPGWAPDPASKILHILEAQYVQLFGDKPKVMAIHAGLECGILGERYPGLDMISFGPTI
                     KHPHSPDEKVHIASVQKFWNLLLHTLANL"
     gene            1777..3108
                     /locus_tag="PLL28_10440"
     CDS             1777..3108
                     /locus_tag="PLL28_10440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69786.1"
                     /translation="MINRVAYLLLLFIPVFGFAQVPAGGGLIVNEINQGTGLKEFMEF
                     VVLGDPLNPCANVDLTGWVFDDNNGTFETCGTGVGIASGHYRFTSCYNAVPPGSILVV
                     YNVADPYVGMPPDDPTDADGDGVYIIPSNSTCLEANYSVPISIPADCNYSGAYSTPTL
                     TWAAGMANSGDVAQVRKPDYSFFHGFSYGDVNTVFPTWPSGADAGTSFNKGSGNLALD
                     CGSCWSSANYITTTAGAGTPGAANTVNNGYFIDNLINCTLNYDDLNDIGNCDLLLPIY
                     QVSLMADVDDHKVQLTWSYTGIIPDNYIIYSSRDGKLFDTIASGTVHEIAEETWTDNV
                     SASGAYYYQIIINDIYGETYHSKIVSANVDVVKQQLFPNPATTTFSLLQHPVHPYTSM
                     YLINMEGETLITAVVEDDASLIKIDISDLPPGHYLVCLTYEHGVDYFPVIH"
     gene            complement(3109..3372)
                     /locus_tag="PLL28_10445"
     CDS             complement(3109..3372)
                     /locus_tag="PLL28_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69787.1"
                     /translation="MNAYLIVYQTDQDISPLRKVLDTMRMVEHISDHSSIVLSFRSAE
                     DILDVIKPSNFEKIPIIIIKLDSVIYTDNIKADFFEPHSVEHL"
     gene            complement(3454..4146)
                     /locus_tag="PLL28_10450"
     CDS             complement(3454..4146)
                     /locus_tag="PLL28_10450"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HNF69788.1"
                     /translation="MIQIEIWSDVVCPFCYIGKRRLERALAGFAHAGQVEITWRSFLL
                     DAQLVTEPGKSINQHLSERKGWSMEQTREINAYVTEMAAGEGLDYNFDKVIVANSVNA
                     HRLLQLAKRNHAGDQVKDALMRAYFIEGRNIDDNKTLQAIGEAAGLPADSVEDLVNDE
                     QQFRRQVFNEAAEAGHFGARGVPFFVFNRKYAISGAQPVEAFSQVLEKVYNESPAIIT
                     ETGDTCTSDGDC"
     gene            4275..6011
                     /locus_tag="PLL28_10455"
     CDS             4275..6011
                     /locus_tag="PLL28_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69789.1"
                     /translation="MLTGHLKAQATYTAADIALDSAVHHYNAYRNTLDAFTPANITDE
                     KINLVESNCNIIVREFESIMALNANPQSEVAKYFHAMTKYEYGFVLGMAGKNDKAYRI
                     MSEIEDYMQKQGSFSFPKKYLYDGKNYVIQWDNFAPTLHEYFTGMAEITINLGMNEKT
                     MPYARSAMSLQYPNTGDNAVWYRYIAASKYMQSSKNIGYYAYDGFEVALNFLDWLYSL
                     DADYQKLVNDYDYPRQVTAYNYILLVEKGYPATNTGENWLKAATAFMKFNESAYAGYC
                     FNRAADLKYDNINFMDQAYAFAKNNNDNSLGLKICEIQESKISADNCSAWTEMAGKWE
                     TFGNQTRASSARAKADACNKAAADAQREYEANQRKQARKDNFDFSMYAGFYPLPLLTI
                     QTNYWDYGGVLGLGLYNMQIEASYKIINRNLVFFEDLSWQEYDTPDQKVFWDGYRAHI
                     AFKFGDRSRYGEGNYVGPLFEIVERNMTNIRSVVSDESTGAYIGELNFEPKETAYNVM
                     VNFGVHIEENHLMADAFMGLGASYIQFSTGAQGYTTDEYLFSDPLLEYRKETRFGPMV
                     RMGMTIGLSTRN"
     gene            6061..6786
                     /locus_tag="PLL28_10460"
     CDS             6061..6786
                     /locus_tag="PLL28_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69790.1"
                     /translation="MKKFILIVCFFPLAIFAQPTIGYLGTLNVGAAYYQEDDMSVFLP
                     DSTATLSNYGVSWGVGVDYIVNRWLIGASFMYFSQPKTSVDTFDIKTKSNDYRLDIGY
                     MTIVRPKFLFGPVLALGGGFDQVNLMSTENATTAQIAANPGREIHLKQNKFVADLGLQ
                     THYRVASGGEGGGLSLGLQVGYSVSYAIADWRFEDAKISDGPEGLRPMMYARLSIGFP
                     SLESDAGDSEPDNGGGDIYHIPN"
     gene            complement(6842..9595)
                     /locus_tag="PLL28_10465"
     CDS             complement(6842..9595)
                     /locus_tag="PLL28_10465"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNF69791.1"
                     /translation="MKLSRSLLIVLLCSLIGFSTLRAQTFKVLNDSCEQLITIDDYPQ
                     ALQACGLAIDKGYSEQVPDSMLMLPRFYLAFALRSLGQREAAVEIMEQLASEILATKG
                     CCDDLVAIYDQLVATYSELNDPKRKLEKMEAIIACETSLHGDSSDFMITYYYLCADDY
                     IITGDYAKSIQYFERAIELYPHFYPDYRNDATYARFLVEYASPLNSVGKTAKAFVLLD
                     TAWAIIQRAPQDETTLNIKNDILQLRVWFYDGIGDAEHAVDAVLQQIEQGEGMNMQTR
                     ANSLVSAALYLINFDIKYGTNVARENNFLDILHNILTVYDSLPPDPIYMSNAYVAIGN
                     YYQVEQIPDSVLHYFRKSSNLIAAAYGKQNVIYLNSLVTQSNYTLLNGDIISAGKILD
                     TVYNLAESFLKSNFLFLSESEQEAVLNDYIPLMNKRNAFYATHLHTFNDVEVALFNSS
                     LFIKGLLLQNITGLRTAVINTGKTDLITLFNEWTNIKTKIAANTVNNKPIPEELTQLA
                     ESLEKKILQSKTLSRTINTSTTVRDISQALEKNSAVIDFMHIISDNDDAYYALVLTSG
                     SHKPQCIRVCVADTLSTILERMPGEREAEYIGRLYTIPDKNFPEDNNYFLGTQLYQLL
                     WEPIRDVLGNVSHLYICADGQLHTIAFHALNDSEGVPLTEHTLITYVNSSRDILHISS
                     DVHPDPVLLVGGLNYSRPNFDPYWRYLPGTLNEIESVESKLNVHNIRFTDYRSDHATE
                     SALIPLNTYYESMHFATHGFAFPAFTDTILPEYGIHFQTSSDPLTRCGLVLSGGDIGW
                     MQQDIQSGIDNILTGNEIANLDLTHTKLVVLSACETSGGDLRGSEGVYGLQRAFRIAG
                     AGALVISLWQVPDQYTTELMQYFYDAYLNGNAPDVALRMAQRNMQKKSQTYYWAAFKC
                     IQG"
BASE COUNT         2874 a         1911 c         2080 g         2922 t
ORIGIN      
        1 aaattttatt aaacaatata attgcattca ctattaatgc gccagcatta aaagcttcca
       61 cacttgctaa cctgctaact tccaaacttg ctaactaaac gaaaatctcc atgcgggtgc
      121 atttctacat cagctaagtg gctaatcggc taattctcaa attaactaat tccccctacc
      181 tttgcatcaa aatcaacacc atgtcatcag cagtccgcaa cctggaaccc aaggctatct
      241 ggaatttctt tgaagatatc aatgccatcc cgcgcccttc taagcatgag gaacgtatca
      301 gcgcttatat ggtcgctttt ggcgagcaat tgggattgcg cacggtgaaa gatgccgttg
      361 gaaatgtgat catctttaaa ccggcaacga caggaatgga atcaaagcct acggttattt
      421 tgcagagtca ccttgatatg gtgcatcaaa aaaattctga tactcaattt gattttaata
      481 ccgaaggtat acgttctcgt atcgacggcg attgggtgaa ggctgagggt acaactcttg
      541 gtgccgataa tggtatgggt gttgctgcca tcatgagttt attggcatcg aaagatattc
      601 cgcatccggc attagaagcg ttgtttacta ttgatgaaga aaccggcatg accggagcga
      661 aagaattgca aaaaggttta ttacagggaa aaatattgtt gaatctggat actgaagatg
      721 atgatgaatt ttctatcggt tgtgcaggtg gtatcgatac caatacttca tacgattata
      781 cacaagaagc acctccaaca ggtgcaatgg catttcgcat tagtgtgagt ggattaaaag
      841 gcgggcacag cggcgttgat attcatctcg aacgcggaaa tgcaaacaag atcatgaaca
      901 gattaatgtt tgatggcgat gcttttggat tacaaattgc aagtatcgat ggtggaagtt
      961 tgcgcaatgc cattcctcgc gagtcatttg caactgttgt tgtaaataat gcaaaatatg
     1021 ctgatgaagt gcgtgctcgt gcagcagcaa tttgccatga atatcattta caggatgaag
     1081 gattgaaaat tgatattcaa tcaactgaaa tgccgggcat ggtgatgtcg tttgacgaca
     1141 gtcgcaaatt aatactggca attcaatcgg cacacaatgg tgtgtatacc atgagcagtg
     1201 ctatttccgg attggtagaa acatcttcat cactggctcg tgtaattgta aaagatggaa
     1261 aattcattac acagagttta cagcgctcat ctgttgaatc ggcaaaaatg gatgttgcat
     1321 ataccgttgc agctcctttc cgcttaatgg gatgttctgt agagcatgga ggcgcttatc
     1381 ccggatgggc acctgatcct gcatcgaaaa tattacatat actcgaagca caatatgttc
     1441 aactctttgg cgataaacct aaagtgatgg caattcatgc cggactggaa tgcggtattt
     1501 tgggagagcg atatccggga cttgatatga tttcattcgg acctaccatt aaacatccgc
     1561 attcacctga tgaaaaagtg catattgcct ctgttcagaa attttggaat ttgttattac
     1621 atacgcttgc taatttgtga ttggaaatgt taaaagatat gttgttaatt atttctcgaa
     1681 ggcatataat acaggtttat taacatcctg taaataaaca gataacaggg catttttacc
     1741 tttgtattct ccaacacaac gacacacacg aaatttatga tcaatcgtgt cgcatatctg
     1801 ttattgttgt tcattccggt cttcggattt gcacaagtgc ctgcgggtgg cggcctgatc
     1861 gtaaatgaaa tcaatcaggg tacaggtctt aaagaattta tggaatttgt ggtcctgggt
     1921 gatccgctga atccttgtgc taatgtggat cttacaggat gggtgtttga tgataataac
     1981 ggcactttcg aaacttgcgg caccggcgta ggaattgcat ccggacatta tcgctttact
     2041 tcttgctaca atgcagtgcc tccgggatcg atattggtgg tatataatgt tgccgaccca
     2101 tacgtgggaa tgccgccgga tgatcctacg gatgcagatg gcgatggtgt gtatattatt
     2161 ccttcaaaca gtacttgtct tgaagcaaat tattcagttc cgatttcaat acccgccgat
     2221 tgtaattaca gtggggcata ctctacacca acacttacgt gggcagcagg catggcaaac
     2281 agcggtgatg tagcgcaggt gcgcaaaccg gattattcat tttttcacgg attttcctac
     2341 ggcgatgtaa ataccgtatt tccgacatgg ccttccggtg ctgatgcagg gacaagcttt
     2401 aacaaaggaa gcggcaacct cgcattggat tgtggcagtt gctggtcgag tgcaaattat
     2461 atcaccacca ctgccggagc aggaactccc ggagctgcaa acactgttaa taatggatat
     2521 tttatcgata atctgattaa ctgcacttta aattatgacg acttaaatga tattggcaat
     2581 tgcgatttat tattgccgat ttaccaagtg tcgctaatgg ctgatgtcga tgatcacaag
     2641 gtgcaattaa cctggagtta tacgggtatc ataccggata attatatcat ttacagcagt
     2701 cgcgacggaa aattatttga tactatagcc tccggaactg tgcatgaaat cgcggaggaa
     2761 acttggacgg ataacgtttc tgcaagtggt gcatattatt atcaaataat catcaatgat
     2821 atttatggcg aaacatatca ttcgaaaata gtttctgcaa acgttgatgt ggtaaagcaa
     2881 caattatttc ctaatcccgc taccactact tttagtttat tgcaacatcc tgttcatcca
     2941 tatacatcga tgtatcttat taatatggaa ggagaaacac ttattacagc tgttgttgaa
     3001 gatgatgcgt cattaattaa aattgacatc agtgatcttc ctccgggaca ttatttggta
     3061 tgcttaacct atgagcacgg agttgattac tttcccgtta tccactaatt acaaatgctc
     3121 cactgaatgt ggttcaaaga aatcggcttt aatgttatct gtatagataa cactgtcgag
     3181 ttttataatg ataataggaa ttttctcgaa gttggaaggt ttgatcacat cgagaatgtc
     3241 ttcggcactc ctgaaactca gtacgatgga gctgtgatcg gaaatatgtt ctaccatgcg
     3301 catagtatca agcacttttc gcaatggact gatatcctgg tcagtttgat aaacaatcag
     3361 ataggcattc ataatacttg ttttattaat gccaaggtag cctatgttac catgttgaac
     3421 agtgacgaat gttaccctta atggtattgt atatcaacaa tcaccatcag aagtacaggt
     3481 atctcctgtt tctgtgataa tggccggtga ttcgttgtag actttttcca agacttgcga
     3541 aaatgcttca accggttggg cgcccgagat tgcatatttg cgattgaaca cgaaaaatgg
     3601 taccccgcga gccccaaaat gacccgcttc tgcggcttcg ttgaatacct ggcgacgaaa
     3661 ctgttgttcg tcattcacca ggtcctccac actgtcggcg gggagtccgg cagcttcgcc
     3721 aatagcttgc agggttttgt tatcatcaat atttctgcct tcgataaaat atgcacgcat
     3781 cagtgcatcc ttcacctgat caccggcgtg attacgtttt gcaagctgta aaaggcgatg
     3841 cgcatttaca gagttggcaa caatcacttt gtcgaaattg tagtcaagtc cttctcctgc
     3901 cgccatttcg gtgacatagg cgttgatttc tctggtttgt tccatactcc agcctttgcg
     3961 ttcggagagg tgttgattga tgctttttcc cggttcggtt accaattgtg cgtcgagtaa
     4021 gaagctgcgc caggtgattt caacctgacc ggcatgcgca aaaccggcca aagcccgttc
     4081 aagacgacgc tttcctatat agcagaaagg acaaacaacg tcgctccata tttcaatttg
     4141 tatcatagtg ctggtaacag ataatctatg ttacaaagtt catataaaat gcattgtaat
     4201 atatctttga tgccatgaaa cttaccggct cctcaaaaat tttaggcaag tgctgtttgc
     4261 tgattgcaat tttcatgcta acggggcatt tgaaggccca ggcaacgtat actgctgctg
     4321 atatcgcttt agactctgct gtgcatcatt ataatgccta cagaaataca ctagatgcat
     4381 ttacacccgc taatattacc gatgagaaaa taaatctggt tgaaagtaat tgcaatataa
     4441 ttgtacgtga attcgaaagt ataatggcat taaatgcaaa ccctcagagt gaagtagcaa
     4501 aatattttca tgccatgacg aagtatgaat atggttttgt gttaggcatg gcaggaaaaa
     4561 atgacaaagc ctatcgcata atgtcggaaa ttgaagatta catgcagaaa caaggcagct
     4621 tcagttttcc aaaaaagtat ttgtatgacg gaaaaaatta tgttattcaa tgggataatt
     4681 ttgcgccgac cttacatgag tatttcacgg gtatggccga aatcaccatc aacctcggaa
     4741 tgaacgagaa gaccatgccc tatgcccgca gcgccatgtc actgcaatat ccaaataccg
     4801 gtgacaatgc cgtttggtat cgatatatcg ctgcttcaaa atatatgcaa tcgtcgaaga
     4861 atattggata ttatgcctat gacggatttg aggtagcatt gaattttttg gattggttat
     4921 atagcctgga tgccgattat caaaagttgg taaatgatta tgattatcct cggcaggtta
     4981 ctgcttataa ttatattttg ttggttgaaa aaggataccc ggcaaccaat acaggagaga
     5041 actggttgaa ggcggcaact gcttttatga aatttaatga aagtgcttat gcgggatatt
     5101 gttttaacag agcggcagat ttaaagtatg acaatatcaa ttttatggat caggcatatg
     5161 catttgcgaa aaataataat gacaacagtt tgggattgaa aatttgcgaa attcaagaga
     5221 gcaaaattag tgctgataac tgcagtgcct ggacagaaat ggcagggaaa tgggaaacat
     5281 ttggcaatca aacacgggcg tcatcggcac gcgctaaagc agatgcatgt aataaagcag
     5341 cagcagatgc ccaaagggaa tatgaagcca accaacgcaa acaggcgcgt aaagacaact
     5401 ttgattttag tatgtatgcc ggtttttatc ctttaccact attgactatt caaacaaatt
     5461 attgggatta tggtggtgtg cttggattgg gattgtataa tatgcaaatt gaagcaagtt
     5521 ataagattat caatcgcaac ctggtgtttt ttgaggatct ttcttggcag gaatatgaca
     5581 cacccgatca gaaagttttt tgggatggct atcgggcaca tatagcattc aaatttggcg
     5641 atcgttcgag gtatggtgaa ggaaattatg tgggccctct gtttgaaatc gtagaacgaa
     5701 atatgacgaa tataagatcg gtggtttctg acgaatctac cggagcatat attggcgaat
     5761 taaacttcga acccaaagaa accgcctata atgtcatggt taattttggt gtgcatatag
     5821 aagaaaatca tttaatggca gatgccttta tgggattggg cgcatcatac attcaattca
     5881 gtacaggagc acagggttat actaccgatg aatatctatt ttccgaccca ctccttgaat
     5941 acagaaaaga aaccaggttc ggacccatgg tgcgcatggg aatgaccata ggcttatcta
     6001 ccagaaattg atcgcgctaa ttgccataca ggtcgtattt ttattattga aatatttatc
     6061 atgaagaaat tcatcctcat tgtttgcttt tttccgcttg caatttttgc acaacctacc
     6121 attggttatc tcggaacatt gaatgttggt gccgcgtatt atcaagaaga tgatatgtct
     6181 gttttcctgc ctgacagcac tgcaacatta agtaattatg gtgtttcatg gggagtaggt
     6241 gtagattata ttgtaaacag atggctaatc ggcgcttcat ttatgtattt ttcacaaccc
     6301 aaaaccagcg tcgacacttt tgacataaaa acaaagagca acgactatcg attggatatc
     6361 ggatacatga caattgttcg tccaaaattt cttttcggtc cggttttggc tttgggtggt
     6421 ggttttgatc aagtgaatct gatgagtaca gaaaatgcca caacagctca aatagcagct
     6481 aaccccggta gagaaattca cctcaagcaa aacaaatttg tagctgatct gggtttgcaa
     6541 acacattatc gcgtagcatc cggaggtgag ggtggtggac tttctctcgg cttacaagtt
     6601 ggctatagcg tttcttatgc catagcagat tggagatttg aggatgcgaa aatatctgac
     6661 ggtcccgaag gcttgcgacc aatgatgtat gcgcgcttat ctatcggatt tccctcactt
     6721 gaatctgatg ccggtgacag tgagccggat aatggcggtg gcgatatcta tcatataccg
     6781 aattaatagt tgtgatgatt gcagtcagtt tacaaccttg taatcaggct tgattagaaa
     6841 ttcacccttg aatacatttg aaggccgccc aataataagt ttgtgatttt ttttgcatgt
     6901 tgcgctgtgc cattcgcagt gctacatccg gtgcgttacc atttaaatat gcatcataaa
     6961 aatattgcat caattcggtg gtatattgat caggcacctg ccataaactg ataacgaggg
     7021 caccggctcc ggcaattcga aacgcgcgtt gcaatccgta tactccttca ctccccctta
     7081 aatcgccacc actggtttca catgcgctta gcacaacaag tttggtatgc gtgaggtcga
     7141 ggttggctat ttcatttcct gttaaaatat tatctatacc cgattgaata tcttgctgca
     7201 tccagccgat atcaccacca cttaatacca aaccacaacg cgtcaacgga tctgatgaag
     7261 tttggaaatg aataccgtat tccggtaaaa tagtatccgt aaatgcaggg aaagcaaatc
     7321 catgtgttgc gaagtgcatg gattcataat aagtattaag tggtatcaat gcagattctg
     7381 ttgcatgatc gctgcgataa tcagtaaagc gaatgttgtg tacattgagc ttagattcaa
     7441 ctgattcaat ttcgttcaat gtgccgggaa gatatcgcca gtaaggatca aaattgggtc
     7501 gactatagtt taatccccct accaataaaa caggatccgg atgtacatca cttgatatat
     7561 gtaatatatc gcgcgaacta tttacatagg taatcaaggt gtgttcagtc aaaggaacgc
     7621 cttcgctgtc attcaaagca tgaaacgcga tggtatgtaa ttgaccatcg gcacaaatat
     7681 atagatggct gacatttcct aatacatcac gaatgggttc ccaaagcaat tggtataatt
     7741 gtgtgccaag gaaataatta ttgtcttccg ggaaattctt gtcgggaatg gtatataatc
     7801 gcccaatata ctccgcctcg cgttcaccgg gcatgcgctc caggatagtt gatagtgtgt
     7861 ctgccacaca tacccgaatg cattgtggtt tatgagatcc ggatgttaat accagtgcat
     7921 aataggcatc atcattgtcg gaaataatgt gcatgaaatc tataacagca ctgttttttt
     7981 ccaacgcctg tgatatatca cgtacagtgg ttgaggtatt aatggtgcga cttaatgttt
     8041 ttgattgcag aattttcttt tccagcgatt cggcaagttg tgtcagctct tcgggaatgg
     8101 gtttattgtt taccgtgttt gccgctattt tagttttgat attggtccac tcattgaata
     8161 aggtaattaa atctgttttc ccggtattga tcactgctgt tcgcaaaccg gtaatatttt
     8221 gtagcaataa tccctttatg aagagcgacg aattgaataa tgcaacttcc acatcattaa
     8281 aagtatgcag atgtgttgca tagaacgcat ttcgtttatt cattaacgga atataatcat
     8341 tcaataccgc ttcctgctcc gattcggata gaaatagaaa attcgatttt aaaaatgatt
     8401 cagcaagatt atatactgta tcaagaattt ttccggcact aataatatct ccattaagta
     8461 acgtatagtt tgactgtgtt actagactat tcagatatat cacattctgt tttccataag
     8521 ccgccgcaat aaggttggaa gatttacgaa aataatgtaa tacagaatcc ggaatttgtt
     8581 ctacctgata ataattgcca attgccacat aggcgttgct catataaatt ggatcaggcg
     8641 gcagagaatc ataaacggtt aatatattat gcaatatgtc gagaaaatta ttttccctgg
     8701 caacatttgt accatattta atatcaaaat taattaaata cagtgcagca cttaccaatg
     8761 agttggcccg ggtttgcatg ttcatgcctt ctccttgttc tatctgctgt aataccgcat
     8821 ccaccgcatg ttccgcatcg ccaataccgt cgtaaaacca tacgcgtaat tgtaaaatat
     8881 catttttgat gttaagcgta gtttcatcct gcggcgcgcg ttgaataatt gcccatgccg
     8941 tatccaacaa cacaaacgct ttagctgttt ttcctacgct gtttaatggt gatgcatatt
     9001 ctacaagaaa gcgtgcgtag gtggcatcgt tgcgataatc cggatagaag tgcggatata
     9061 gttcaattgc gcgctcaaaa tattgaatag atttggcgta atcgcccgta ataatataat
     9121 catctgcaca gagataataa taggtaatca tgaaatcaga tgaatcgccg tgcaaagatg
     9181 tttcgcatgc aatgattgcc tccatctttt ccagctttcg tttcggatcg ttcaattcag
     9241 aatacgtggc aaccaattga tcgtaaatgg caacaagatc atcacaacat ccttttgtag
     9301 caagaatttc tgatgcaagt tgttccatta tctcaacggc ggcctcgcgt tgacccaatg
     9361 agcgcaatgc aaaagccaag taaaatcgcg gaagcatgag catagaatcc ggtacctgtt
     9421 cgctgtatcc tttgtcaatt gctaatccac atgcctgcag cgcctgagga taatcatcaa
     9481 tggtaatcaa ttgctcgcaa gagtcattca atactttgaa tgtttgtgcg cgcaaagttg
     9541 aaaagccaat taagctacac aataaaacaa tcagtaaaga cctgctcaac ttcatgattt
     9601 tcaaagatag tggtgtaaaa ttatcttatt gatatggaaa ctcgcatcat attgcatctt
     9661 tatttaaatt ccaaaagatg cttcaataac tacccaagaa tcatcgggcg tttgcctgta
     9721 gtaccttatt tttatcactg ctaatgcact tacatatgaa gaaacacatg ttaacctttg
     9781 tggctgc
//
LOCUS       DAOQHY010000125         9497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_3661_length_9497_cov_4.622220, whole genome shotgun sequence.
ACCESSION   DAOQHY010000125 DAOQHY010000000
VERSION     DAOQHY010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9497
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3661_length_9497_cov_4.622220"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PLL28_10470"
     CDS             complement(<1..355)
                     /locus_tag="PLL28_10470"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNF69792.1"
                     /translation="MKKIYSLIFALCVATLTFAQQPAFITDSLDSYIQREMKKWNIPG
                     MAVAIVKDGQIVVEKGYGVTDVHTGQKTDQNSLFMVASNSKAFTGTALSLLEYQKRLN
                     LNDKVTTYLPYFKLYD"
     gene            complement(352..978)
                     /locus_tag="PLL28_10475"
     CDS             complement(352..978)
                     /locus_tag="PLL28_10475"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HNF69793.1"
                     /translation="MSEISRIPLLLEPHYFPCISWWAAVSRHESVVLDVSAHYQKGSF
                     RNRAHILGSNGILRLSIPLEHGRTQRKTTGDVKICYDEDWQKIHWQTLISSYRRSPFF
                     EYFEDILEALFVRPFTFLIDMQMAVIAIMKKLIGIETQFTLSTHYIDPGDPQYVDLRN
                     TIHPQKTSQLNFIRYTQVFSDRFAFIPDLSIIDAICNKGKITINDLIL"
     gene            1032..2519
                     /locus_tag="PLL28_10480"
     CDS             1032..2519
                     /locus_tag="PLL28_10480"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HNF69794.1"
                     /translation="MRNLYLFTILICLTGFSAQAQSLSTNTAVRSCLFLHPTEGAFAE
                     VYLVAGANALRPAAGNNGFTAGVDVSILVIDAAKGNSEGSNVLNYDKFRLNTPVFKDT
                     NLVDFSIIDQKRLAIPNKTVTVEVTVTDINDPSNYFVSTEVLTSYQENMIQISDIQFA
                     ESYKPVDKENNFSKNGVELMPYPLTFFPSTKSSIILYGEVYGADKYLQDDQFLVAFSI
                     KNASTNEFNPTFYQYVKAEKQPVFSFLREINIADLPSGNYNLYVEVRNKKNELITQKV
                     VYIQRANTGPVYAWENIKMINTAGTFTEEYSEEQLDYFLDVIRPRATESDLNLIESLS
                     GRVEPDMKRKFLYNFWVERNAADPYGEWKKYLALVKEANNSFGTPSRQGYKTERGRVY
                     LQYGPPYDVVTSVNEAGAYPYEIWFYTTLPDKQTNIGFAFYEPTMVSNDYILLHSNAR
                     GELHEPRWKVKLYENVASPQEVLNFDNTEVEDKIGGHRAVDMYQF"
     gene            2582..3643
                     /locus_tag="PLL28_10485"
     CDS             2582..3643
                     /locus_tag="PLL28_10485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNF69795.1"
                     /translation="MQADDILQRPAVAIVILTWNGRKFLEQYLPSVVRLPYQPLDIYI
                     ADNASTDDTVDFVKANYPSIKIIAIPKNEGFAKGYNVALKQVVADYYFLVNQDVELTP
                     NCLEPMIEIMESNVMIAACQPKILAAWDKSRFEHAGAAGGYIDAFGYPFCRGRLFNIE
                     EQDTGQYADAREIFWASGAALLIRAELFNRFKGFDPDFFAHMEEIDLCWRLKRAGYQI
                     HYIPEATVYHVGGGSLPKENPYKIYLNFRNNLSMLFKNYETTDLIWRFPVRILLDNAA
                     FFHALFNGRRKEAGAIFRADWHFVIALPLHMKKRFDTWRQYHRNRVAVSRVKMSGYYN
                     KSIVWQFFARGKKYFSELP"
     gene            complement(3640..4059)
                     /gene="aroQ"
                     /locus_tag="PLL28_10490"
     CDS             complement(3640..4059)
                     /gene="aroQ"
                     /locus_tag="PLL28_10490"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNF69796.1"
                     /translation="MRILILNGPNLNLLGVREPEIYGSESFETFFAKLQQRFPNVELI
                     TMQSNHEGALIDALHAYGFSYDGIIINAGGLSHTSIALADAISAITTPVVEVHISNTF
                     ARESFRHHDVLTTNCKGIIAGLGLEGYALAVKYFLSH"
     gene            4143..5045
                     /gene="xerD"
                     /locus_tag="PLL28_10495"
     CDS             4143..5045
                     /gene="xerD"
                     /locus_tag="PLL28_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNF69797.1"
                     /translation="MNWNGAIKEFKGYLRLEKSLSPNSVEAYIRDITKLHQFLEGNFP
                     GTIPEKVDRQQLEGFLIFLHEVGMNDTSQARIISGIRAFYKFLLIEDLIDHDPAHLID
                     LPKLRRKLPDTLSYEEVMSIISAVDLSKKEGQRNKAMLETLYSCGLRVSELIGLRISD
                     MFTEDGFIRVRGKGDKERLVPIGDSAINYIKIYKETVRSHITPQKGFEDHVFLNNRGK
                     MLTRVYVFMLIKQLAEQAGIKKNISPHTFRHSFATHLIEGGADLRAVQEMLGHSSITT
                     TEIYTHIDREFLRSTLIQYHPRFK"
     gene            complement(5853..7982)
                     /locus_tag="PLL28_10500"
     CDS             complement(5853..7982)
                     /locus_tag="PLL28_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNF69798.1"
                     /translation="MKTYSSEHIKNVVLVGHSGSGKTTLAECMLFEAGAINRRGTVSE
                     QNTTSDYNELEHERGNSVFSTVMHVDWKDTKINIIDTPGMDDFTGEVIAAFKVADTGL
                     LVLNAAHGVEVGTELIWEYTDHLKKPMILVVNHLDEDKADFENTVAQAKERFSRKVTV
                     VQYPYNQGLDFNAIIDVHTMKMYRFPADGGKPEKLPIPESEMEKAQRLHNELIETVAE
                     NDDTLMEKFFEKGTLEEEEMMKGMMLSLRHHDLFPVFCASAKHNMGSGRIMGFIDTCC
                     PSAAHMQPEKLTNGADLPCNSSNPTVAFVFKTVSEPHLGDMSFFKVCSGKVTSGMDLN
                     NTDTNSPERITQLFVLQGKKREQVNELLAGDIGATVKLKKTHTNNTLAIKGQDVQIVP
                     IHFPEPKVQAAIDVKNKGDEEKVGLALHHIHEEDPTVEIDHNMELKQTIINAQGELHL
                     QVLKWKIAHSYGVNIDFMEPRIPYRETIQKAVRADYRHKKQSGGAGQFAEVHMLVEPW
                     YEGMPDPPELNVRGREEHDLKWGGKLVFFNCIVGGAIDARFLPSILKGVMSKMENGPL
                     TGSYVRDVRVSVYDGKMHPVDSNDMAFKLAGMMAFRNAFREADPKILEPIYNLEVLVP
                     GDYMGEVIGDLQTRRGIVMGMDNDGHYQKIIARVPLAELYDYSSSLRSLTQGRGKFVR
                     SFAEYAAVPYEVQTELMASHKEEPAEA"
     gene            8410..9096
                     /locus_tag="PLL28_10505"
     CDS             8410..9096
                     /locus_tag="PLL28_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNF69799.1"
                     /translation="MQVTYYGHSCFGVEVGGKHLLFDPFIRPNPLAAEIRVEDIKADF
                     ILLSHAHVDHTADALEIAKRTGAVIIGIWEIHSWAERNGVKNSHPMNIGGSFTFSFGT
                     VKMTSAVHSSSFADGSYGGSPAGFYIETAEGNFYYAGDTALHNDMKLLGKYANIDFAF
                     LPIGSNFTMGVDDALVAAKFIKCDQIIGMHFDTFGYIKINKSEAIAKFKERDRNLILM
                     EIGETIEVTQ"
     gene            9126..>9497
                     /locus_tag="PLL28_10510"
     CDS             9126..>9497
                     /locus_tag="PLL28_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF69800.1"
                     /translation="MGKIIAIANQKGGVGKTTTAINLAASLAVLEFKTLLVDADPQAN
                     STSGVGFDPKENRVNIYDCLMNDKHAKDAIVETETPNLHLLPSHLDLVGAEIELINHP
                     NRERILKSVLDEVKNNYDFIFI"
BASE COUNT         2769 a         2004 c         1954 g         2770 t
ORIGIN      
        1 gatcatagag tttaaaatag gggagatagg tagtaacctt atcattcaga tttaatcgct
       61 tttgatattc gagcagcgat aatgccgttc ctgtaaatgc tttgctgtta ctggcaacca
      121 tgaacagcga attttgatcg gttttttgtc cggtatgcac atcggtaacg ccatatcctt
      181 tttctacaac aatttgtccg tccttaacaa tggctactgc catgcccggg atattccatt
      241 ttttcatttc gcgctggata tagctatcca aactatcggt aataaatgcg ggttgctgag
      301 cgaatgtcag tgtagcaaca catagtgcga aaatcaggct atatattttt ttcatagtat
      361 cagatcgtta atggtgattt tacctttatt acatatggca tcgataatac tcaggtcggg
      421 aataaatgca aatctatcac tgaatacctg cgtatagcga atgaaattta attgcgatgt
      481 tttttgtggg tggatggtat tcctcaaatc tacatattgt gggtcgcccg gatcgatata
      541 atgtgttgaa agggtgaatt gcgtttcaat acctatcaat tttttcatta ttgcaatcac
      601 tgccatttgc atgtcgatga gaaatgtaaa tggtctgaca aacaatgctt ctagtatatc
      661 ctcaaaatat tcaaagaaag gggagcggcg gtaactgctg atgagggttt gccaatgtat
      721 cttctgccaa tcttcatcat aacagatttt tacatctccg gtagttttcc gttgagttct
      781 gccatgttcg agaggtatac ttaatcgcag gatgccattt gatccaagta tatgtgctct
      841 gttgcgaaag ctaccttttt gataatgtgc agaaacatcc aaaaccactg attcatggcg
      901 cgatactgct gcccaccacg atatgcaagg gaaatagtgc ggttctaaca atagtggtat
      961 acgggaaatt tctgacatcg ttgtgacgtg tgtatctaaa tatgctgtaa ttttaagatt
     1021 atttttcggc tatgagaaac ctatacctgt tcactatcct catttgttta accggctttt
     1081 ctgcgcaagc acagtcactt tcgaccaata ccgctgtcag aagttgcctt ttcctgcatc
     1141 ctaccgaagg tgccttcgca gaagtgtacc tggttgccgg agccaatgca ttgcgtcctg
     1201 cagccggaaa taatgggttc actgcgggtg ttgacgtatc catattggtg atagatgccg
     1261 ctaaagggaa ttccgaagga agtaatgtct tgaattacga taaattccga cttaatactc
     1321 ctgtatttaa ggataccaat ctggttgatt ttagcattat cgatcagaaa agactggcaa
     1381 ttccaaataa gacagttacc gtagaagtta ccgtaaccga tatcaacgat ccgtctaatt
     1441 attttgtatc aacagaagtg ctgacatcct atcaggaaaa catgatacag atttcagata
     1501 ttcaatttgc cgaatcttat aaaccggtag ataaggaaaa taatttctca aaaaatggtg
     1561 ttgaattgat gccataccca ttgacatttt tcccgtctac aaaatcaagc atcatattat
     1621 acggtgaagt gtatggtgcc gacaaatatt tgcaggatga tcagttcctg gtggcattta
     1681 gtatcaaaaa tgcatcgact aatgaattca atcctacctt ctatcaatat gtgaaagctg
     1741 aaaagcaacc tgttttctca tttctgcgcg aaataaatat tgcggatctg ccaagtggta
     1801 attacaactt atatgttgag gtccgcaata aaaagaatga actcatcaca caaaaagtag
     1861 tgtatattca acgagcaaat acaggtccgg tttatgcttg ggagaatatt aaaatgatca
     1921 ataccgcagg cactttcacg gaagaatatt ctgaggagca attagattat tttcttgatg
     1981 ttattcgtcc aagagctacc gaatcagatc ttaatcttat tgaatctctc tccggtcgcg
     2041 ttgagccgga tatgaaaaga aaatttttat ataatttttg ggtggaaaga aatgctgctg
     2101 atccttatgg tgaatggaaa aaatacctgg cacttgttaa agaagctaac aacagtttcg
     2161 gcacgccatc gcgacaagga tataaaactg agaggggacg ggtttacctg caatacggac
     2221 ctccgtatga cgttgttact tcggtaaatg aagcgggcgc ttatccatat gaaatctggt
     2281 tttataccac acttcccgac aaacaaacga atattggatt tgcattctat gagccaacga
     2341 tggtgagcaa cgactatatc ctcttgcatt ccaatgcacg cggagaactg catgaaccac
     2401 gctggaaggt caagttgtat gaaaatgtag cctcccctca agaggtgttg aatttcgaca
     2461 atactgaagt ggaggacaag attggcgggc atagagccgt ggatatgtat cagttttaaa
     2521 gaattccgga cgtttttcgg cgtcccttgg cattcgttcc actttcctat ctttaccctg
     2581 catgcaggca gacgacattt tgcaaagacc ggcggtggcg attgtaatac tcacctggaa
     2641 cgggcgtaaa ttccttgagc aatacctgcc atctgtagtt cgtttgccat atcaaccact
     2701 tgacatttat attgccgata atgcttctac cgatgatacc gttgattttg taaaagcaaa
     2761 ttacccttca attaaaatca ttgccatccc caaaaatgaa ggctttgcga aaggatataa
     2821 tgttgcatta aaacaggttg ttgccgatta ttattttttg gtgaatcagg atgttgagct
     2881 tacacccaac tgcctcgagc caatgattga aataatggaa agcaatgtaa tgattgcagc
     2941 ctgccaacca aagattcttg cagcatggga taaatcgcgt tttgaacatg ccggtgcagc
     3001 aggtggttat atcgatgcat tcggttatcc gttttgcaga ggaagattat ttaacattga
     3061 agaacaggat acaggtcagt acgcagatgc tcgtgaaata ttttgggcta gtggcgcggc
     3121 attattgata cgtgcagaac tattcaatag gtttaaaggc ttcgacccgg atttttttgc
     3181 ccatatggaa gaaattgatc tctgctggcg attgaaacgc gcgggttatc aaattcacta
     3241 tatccctgaa gcaacagttt atcatgttgg cggcggaagt ttgccgaaag aaaatcctta
     3301 taaaatatat ctcaacttca gaaataacct gagcatgttg tttaagaact acgaaacaac
     3361 agatttgatt tggcgatttc cggtaagaat attattagat aatgctgcct tctttcatgc
     3421 attgtttaat ggccgccgga aagaagcagg cgcaatattt cgggccgatt ggcattttgt
     3481 tattgcactg ccattgcaca tgaaaaaacg ttttgatacc tggcgccaat accatcgcaa
     3541 tcgcgtagca gtaagcaggg tgaaaatgtc gggatattat aataaaagca tcgtttggca
     3601 gttttttgcc agaggaaaga aatattttag cgagctgcct tagtgcgata aaaagtattt
     3661 cactgcgagg gcatatcctt caagtcccaa gccggcaata atacccttac aatttgttgt
     3721 gagcacatcg tggtgcctga atgattccct ggcgaaagtg tttgaaatat gcacttctac
     3781 aacaggagtg gtgatggcgc taatagcatc ggcaagtgca atgctggtgt gcgataatcc
     3841 acctgcattg ataataatgc catcgtaact aaaaccataa gcatgtaatg catctatcag
     3901 ggcaccttca tgattgcttt gcatcgtgat caattccaca ttcggaaaac gctgttgcag
     3961 tttggcgaaa aaggtttcga aactttcgct gccataaatc tccggttcgc gaacacctaa
     4021 caggttcaaa ttcgggccat tgaggatgag aatacgcata actggacaaa tttacgatta
     4081 ttcacacagc aaaatagtat atcggagatg cccgttttta gtgtcgaatg taacttaaca
     4141 ccgtgaactg gaatggcgcc atcaaagaat tcaaaggtta cctgcgactc gaaaagtcct
     4201 tatcgccaaa ttctgttgag gcttatatcc gcgatatcac taagttgcac cagtttcttg
     4261 aaggcaattt tcccggaacc atacctgaaa aggttgatag acagcaatta gagggttttc
     4321 tcatttttct gcatgaggtc ggcatgaatg atacttcgca ggcaaggatc atttcgggga
     4381 ttcgggcttt ttacaagttt ctccttatag aagaccttat tgatcatgat cctgctcacc
     4441 tcatcgacct gccaaaactt cgacgaaaat taccggatac gcttagttac gaagaagtga
     4501 tgtccattat cagtgcagtt gacctgagca aaaaagaagg tcagcgcaat aaggctatgc
     4561 ttgaaacctt gtattcgtgc ggattaaggg taagtgaatt gataggctta cgcatttccg
     4621 atatgtttac cgaagatggt tttattcgcg ttcgtggcaa gggcgacaag gagcgactgg
     4681 tgccgatagg ggacagtgcc atcaattata tcaaaattta caaggaaact gttcgaagtc
     4741 atatcacccc tcaaaaaggt tttgaagacc atgtgtttct gaacaaccgg ggtaaaatgc
     4801 tcaccagagt atatgttttc atgctcatca aacagttggc ggagcaagct ggcattaaaa
     4861 agaatatctc accccatact ttccgtcact ctttcgccac acacctgata gaaggaggcg
     4921 ccgatttgag ggccgttcag gaaatgctcg gacatagctc tatcaccacc accgagattt
     4981 atacccatat tgacagagaa ttcctgcggt cgacattgat tcaatatcac ccaaggttca
     5041 aataagattg ccaagatttt aactaaaaaa caggttaatg ggcaaaaaaa aagcagagtg
     5101 cacggaagaa ctccgattcc tctgctatcc ccatgaaaac acaaaacttt atgaagatgc
     5161 ggttttagcc gccgttcaat tcaaaaattg taaataaata acgtttaaca gcacaaatat
     5221 acaatcaatg cgctgaataa tccaaatttt ttaaaaccgg caacttaatt taacaatgta
     5281 gttttcaacc ggaaaagcac attgtatccc ttaaaacatg caattgagtt taaagtgtgc
     5341 gattgattat ggttttcagc cgaaatgcaa tcacggcaaa tgtttcccgg caggttataa
     5401 aattattttc tgttcaaaaa aacctctcga gtgtacccgg cttgatggaa tacatgtatc
     5461 ctgaaaccgg tggaggctag tacacaaatc cgtctttaaa gtaaggttcc gggggttttc
     5521 gtcgaattat gttatcgtaa cgaatctcgg gttgtatagc ataaaaatat atgtgttgac
     5581 cacgatcaat tccttatgta ctatttcaca caaactcaca tgttttagca aagcatcttt
     5641 gcattgttgc taacaaattg caacatgcat tattacaccg ctcacgcctg agaacggtga
     5701 ataatatggc caatagtagt atgttttgat gcttgtaatc agccgaggta aacacctcgg
     5761 ctgattcgct ttgtataaat acaatggttt acagcaaaaa aaagcaacag tctcctcaaa
     5821 ctgttgctct tattaaaatg atgcttttat aattatgctt ctgcaggttc ctccttatgg
     5881 cttgccatca attcggtttg cacttcataa ggcactgcgg catattcggc aaaactgcga
     5941 acgaattttc ctcggccctg agtaagggaa cgcaggctac tggaataatc atacaattcg
     6001 gccaaaggaa cacgagcgat gatcttttga tagtgaccat cgttatccat gcccattaca
     6061 attcccctgc gggtttgcaa atcgccaatc acttcaccca tataatcgcc gggcactaat
     6121 acttcgagat tgtaaatcgg ttcaaggatt tttggatcag cttcgcggaa ggcattgcgg
     6181 aaagccatca tacctgccag tttaaaagcc atgtcattgc tgtcgaccgg gtgcattttt
     6241 ccatcataca cacttacacg tacatcgcgc acataagaac ctgtaagcgg accattctcc
     6301 attttgctca tcaccccttt taatattgaa ggcagaaagc gagcatcgat ggcaccaccg
     6361 acaatgcaat tgaagaaaac gagtttacca ccccatttca aatcatgttc ttcgcgacca
     6421 cgcacgttca attccggtgg gtcaggcatg ccttcatacc aaggctcaac aagcatgtgc
     6481 acttcggcaa actgaccggc gccgccactt tgttttttgt gtcgataatc ggcgcgcact
     6541 gctttttgta ttgtttcgcg gtatggaatt cgcggttcca tgaaatcgat gtttacaccg
     6601 tagctatgcg ctattttcca tttcaacact tgcaagtgga gttcgccctg agcattaata
     6661 atggtttgct tgagttccat attgtgatcg atctctacag taggatcttc ttcatgaata
     6721 tgatgcagag cgagtcccac tttttcttca tcacctttat ttttaacatc aatggcagct
     6781 tgcactttag gttcaggaaa atgtatggga acaatttgaa catcctgacc ttttattgcc
     6841 aaagtattat tggtgtgcgt ttttttcaat tttacggtgg caccaatatc acccgccaat
     6901 aattcattca cttgttcgcg ctttttgccc tgcaatacaa ataattgtgt gatgcgttca
     6961 ggactattgg tatcagtatt atttaaatcc attcctgatg tcaccttgcc actgcatact
     7021 ttaaagaagc tcatatcgcc taggtgaggc tcacttacgg ttttgaatac aaatgctact
     7081 gtcggattag atgaattaca cggaagatct gcgccgttgg taagtttttc aggctgcata
     7141 tgcgctgcgg aagggcagca ggtatcgata aaacccataa tgcgaccgga tcccatattg
     7201 tgtttcgcag aagcgcagaa taccggaaac aggtcgtggt gacggagtga taacatcatc
     7261 cctttcatca tttcctcttc ttccagtgtt cccttctcga aaaacttctc catcagcgta
     7321 tcatcatttt ctgcaactgt ttcaatcaat tcgttgtgca gacgctgcgc tttttccatt
     7381 tcagattccg gtatcggcaa tttttccggt ttaccgccat ctgcagggaa gcgatacatc
     7441 ttcatggtat gcacatcaat gatggcattg aagtctaatc cctgattata tggatattgc
     7501 accacggtaa cttttctgct gaatcgttcc ttggcttgtg caacggtatt ttcaaaatca
     7561 gctttgtctt catccaggtg attaacaaca aggatcatcg gttttttcaa atggtcggtg
     7621 tattcccaaa tcaactctgt tcctacttct acgccatgag cagcatttaa cactaataaa
     7681 ccggtatcgg caactttaaa tgcggctatt acttctcccg taaaatcgtc catcccggga
     7741 gtatcaataa tatttatttt ggtgtctttc caatctacat gcatcacagt gctgaatacg
     7801 ctattccctc gttcgtgttc cagctcatta taatcagaag tagtattttg ttcagataca
     7861 gtacctctgc ggttaatagc tccggcttca aacaacatgc attcagccaa cgttgtcttt
     7921 ccggagccgg aatgccctac caatacaaca tttttgatgt gttcactgct ataagttttc
     7981 ataacgtcct ccttggttta gacctgcaat ataaagaaac cgtaaaacaa tcggaaatga
     8041 cataaatcat aagttcacct gcgaagaaat cacctaatcc ttcaatatgc atgcaggttt
     8101 tagcccgcag acaccaaaac gcgccatttt caaccccgaa actttaacat ttcaattccg
     8161 gcaatccttt caaagcctca ccgtgacttt cctcaccacc cttagcgcct tcgcacctta
     8221 ccgccttggc gcgaaaaaca tacatcatat acttaaacta taaaccactc cttaaactct
     8281 ttttccatac ctcaaaaccc actaaaactt atccacagag cgtttagatg ctgttaaaaa
     8341 gttattgcct tttgggtggg taagggcgaa ctaagcgcgt aattttacac ccgttcagca
     8401 caaacgatca tgcaagtaac ttattacgga cattcatgct ttggcgtaga ggtaggaggg
     8461 aagcatctgc ttttcgatcc tttcattcgt cccaacccgc ttgctgctga aatacgtgtt
     8521 gaagatatca aagccgattt catcctattg tcgcatgcgc atgtcgatca tactgccgat
     8581 gcactggaga ttgccaagcg aacaggagcg gtaatcatcg gtatttggga gatacactcc
     8641 tgggctgaac gcaatggggt aaaaaactca catccgatga atatcggcgg tagtttcact
     8701 ttcagcttcg gcactgtaaa aatgacctct gcggtgcata gcagctcatt tgcagatggt
     8761 agttatggtg gcagcccggc aggattttat atcgaaacgg cagaaggtaa tttctattat
     8821 gccggcgaca cggcgttgca caacgatatg aagttgctgg gaaaatatgc caatatcgac
     8881 tttgcatttc ttcctattgg tagcaacttt accatgggcg tggatgatgc attggtagct
     8941 gcgaaattta tcaagtgcga tcagattata ggtatgcatt ttgatacctt cggatatatt
     9001 aaaatcaaca aatcagaagc tattgcgaaa tttaaagaac gcgaccgcaa tttgattttg
     9061 atggaaatag gcgaaactat agaagtaaca caataacagt tcatcattaa aaggaaacga
     9121 attacatggg aaagatcatt gccattgcca accagaaagg gggagtagga aaaacaacaa
     9181 cagcaatcaa tctggctgca agtctggctg tgctggaatt taagacattg cttgtagatg
     9241 ccgatccaca agcaaattca accagcggag ttggctttga tcccaaagaa aatagggtga
     9301 atatctacga ctgtctgatg aacgacaaac atgcaaaaga tgctatcgta gaaacagaaa
     9361 caccaaattt gcatttattg ccttcgcacc ttgacttggt gggtgctgaa attgaattaa
     9421 ttaatcaccc gaatcgcgaa cgcattttga aaagtgtact cgatgaggtg aaaaataact
     9481 acgacttcat ttttatc
//
LOCUS       DAOQHY010000126         9437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_3694_length_9437_cov_5.272117, whole genome shotgun sequence.
ACCESSION   DAOQHY010000126 DAOQHY010000000
VERSION     DAOQHY010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9437
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3694_length_9437_cov_5.272117"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="PLL28_10515"
     CDS             complement(<1..726)
                     /locus_tag="PLL28_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69801.1"
                     /translation="MRKLLLTVAAGVISVSLFAQTAVTAKPHNKAEMMAKFKNAKAVP
                     YQPQVNADLNGTPLNTNTFYAKSGARTGESIGITGYPLMSNSASYDRVRVYDDGAISA
                     IWTGSTVADGSWADRGSFYNHNDGASWGPAPTARVETVRTGFPELLTVMDHEVVIAHD
                     GTNQRLFANASIGGTTWTELPTSGMHHGLWPRAYCPAGTDDIYLVSPNASPVVTINFS
                     RSDDGGATWTVLNDVLPLDSTNCF"
     gene            complement(937..1245)
                     /locus_tag="PLL28_10520"
     CDS             complement(937..1245)
                     /locus_tag="PLL28_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69802.1"
                     /translation="MAKTTKKAAGKRGRPAGKKAAKKAAPKKAAKKAAPKKAAKKAAP
                     KKAAKKAAPKKAAKKAAKKAAPKKAAKKAAPKKKAGKRGRPAAKKVAAPAAPANGATN
                     "
     gene            complement(1433..1930)
                     /locus_tag="PLL28_10525"
     CDS             complement(1433..1930)
                     /locus_tag="PLL28_10525"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNF69803.1"
                     /translation="MKELLNSYCDYNVWANTAIIQTLSSVSPEIIDKHMGGSFPTIRK
                     TLYHIWDAQVIWLSRLQGSSPSDWPSAMYDDEFAGYDLYFIQQSEDFARFVSSRPEDF
                     FGDMCFYKALNGQEYQTRNGEIILHCMNHGSYHRGQIINYLRMQGVSDLPHTDYIFYS
                     RAKQS"
     gene            complement(2022..3311)
                     /locus_tag="PLL28_10530"
     CDS             complement(2022..3311)
                     /locus_tag="PLL28_10530"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HNF69804.1"
                     /translation="MKVVCILLSTPIYIGVSAQSDSTMIPEVKKGLVEMVSDPMIRTV
                     QLQYSTLELAYPILPLNTDGQFLTLSFDDLHGGVKDYYYTFTHCDQQWEPSGLTSFDI
                     IDGFDESRISDYQFSFGTLQRYTHYAVSFPNEDVQFRLSGNYIVQVWKDEDRDTPILT
                     KRFFVWEQEAAIDAKVMRPNLIPYRNEFQEINFNVDVRNIDVHDAFDDIRVTVLQNGR
                     FDNASYNLKPRMVTNDMLMYDIDDLVFPGGKEYRRFDTKTLKFQTDRITKIQQQDGQY
                     HVYVNVDESRVYQRYTYEKDANGQYVIQAELSDDVNKEGDYAWIHFTLQYPYVITSGN
                     VFVIGEGMQGDTTLQAIPMTYNYDRQQYEAKAYLKQGYYNYQYALLPQGASYYALDYT
                     EGNTYESENDYVVLVYLHMFDKDYDRLIGCTVVNSVK"
     gene            3406..4185
                     /gene="mazG"
                     /locus_tag="PLL28_10535"
     CDS             3406..4185
                     /gene="mazG"
                     /locus_tag="PLL28_10535"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNF69805.1"
                     /translation="MQTSKEAKLEAFSRLLDIMDDLRSQCPWDKKQTIHSLRNLTIEE
                     TYELADAITGNDWKGIKEELGDILLHMIFYSKIGEEQEQFSIADVLHAQCEKLIHRHP
                     HIYGDVKVQDEDEVKRNWEQLKLKEGKRSVLQGVPNGLPALIKAYRIQDKAGQVNFEW
                     DHIDQVWVKVREEMQELQEAKDPDHVEQEFGDLMFALINLSRYLKIDPENALERTNKK
                     FISRFSYIEEQAAGMQKNLRDMSLAEMDALWDKAKAKEREA"
     gene            complement(4198..4902)
                     /locus_tag="PLL28_10540"
     CDS             complement(4198..4902)
                     /locus_tag="PLL28_10540"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HNF69806.1"
                     /translation="MSWLRNIFRKKLPAKGILLYGLQRSGTNYMETLLQLNYPDGLLL
                     NGTQRNEITHKHFRLYKEKEIIPEPQFANTLLVPTLKDFEAALPFQAPDLYIIVSKDP
                     YSWLGSYNKWSIKNNWPAHQHHYIMEYVLFYKMWMNYSREAKNILFVRYDDLLTDPAM
                     VMKQIAQVVEWPELEKVQSTNKVYASRRFNNEKKIAHLSGDQIKKINPQELRQINEML
                     PENLMEFMGYKKLMVN"
     gene            complement(4976..7906)
                     /locus_tag="PLL28_10545"
     CDS             complement(4976..7906)
                     /locus_tag="PLL28_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF69807.1"
                     /translation="MRNVCTLLIMFIMLLGAQAFGQNRTVTGVVKDAATGETIPGASV
                     MTKEDRSLGTATDINGEFKLEVPANTQALMVRSVGYAEIEVALTTSAKYEVMLTSEDY
                     NLDAIVVSASRRQEKILDAPASITLISPEQIKNTVAITPIQNIKGVPGVDIINTGLVQ
                     TNVVVRGFNNIFSGALLTMVDNRYAAVPSLRANVATFIPTDNLDLERIEVLRGPASAL
                     YGPNCSNGVFHMITKSPLSQEKKYMTSASLGLGFRSMISDTIMIANPIDPATGLVYGD
                     YTPLFDYDNIGERTFYTAGFRHSGKVNDQFGYRILFTYLNGMDWLYDDPFEPAQVQLG
                     YQTSEGRQNVGDTIDNTRDSHIEKIGVDLRFDYRLKNKAEFVLSGGMTQNDGIELTGI
                     GAGQAVGWKYYYAQLRYIHNDWFVQGFVNGSDAGDTYLFRTGDLIIDHSKQYVGQIQH
                     ANDWMDKKLNLVYGLDAIMTNPDTENTINGENEDDDNIIEIGAYAQGDYMVNPKWSLV
                     GALRYDYHNFVNNPFLSPRAALVYKANNKNTIRLTYNRSFSAPSSNNLNLDILQLGDL
                     TGYAGLFQGYLASSYVPNMPLYAIGNRDGFHYSYDDFGLPQYTSPYSAYLGYASDHYF
                     SLDNSYELNNVIWDLTLGLLEDAFDATIPGLGAALVAWLPPEIPVTSNNIAQLNLTTE
                     EFETIDPTNITDYSSMLNSATNTIEVGWKGSMFNDKVFATVDVYYNMMQDYVSPLTDI
                     TPNVFIDGAELAAYVAPIITENYYDTTGSNEFLASLVTATLDDDPAYGGNNNGTGVDE
                     FINLVLAAGAGLPVGTISPMEYPDGAKYVTYVNLGDIAVLGTDIGVSWYVNDDLTMQF
                     SYSWVDKDSVELEGAQLGYVALNAPKNKVGVRGSYDIKKADLNVGLTWRWQSSYPANS
                     GAYVGTVEEIHDMDLTLNYTPDFIKDTQITMLITNLYNHEQQYFVGAPKIGSTYMMKV
                     TKTF"
     gene            8062..9357
                     /locus_tag="PLL28_10550"
     CDS             8062..9357
                     /locus_tag="PLL28_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69808.1"
                     /translation="MKIRRFHIILPIVFALCAITAYVIAGRAERSQSLEATAKHIEHD
                     YQTLEREIIAVISDEMIISNLITDSYTFEEFSRLAERPYGIVVYQNDSALIWTNNFIT
                     PIKAQVPFNDAPLLISESNGEYIIFQHDFPGDVHVIGFLPLQFEYGIRNKYLDHVKSP
                     GMHIPDFVRINPRAEGASTPVMSNSGNAVFYVSNDGLETAATVYPQSMFVMLILSALF
                     WLMCMYQLARWIAFKFGRLPAVGALLILLSLSYILLRDLFYDNVLSALPLFDPQFYGS
                     PGIAGSTGDLLIKTVWLFCFALFFSRFFFIKQLRSVWLKATLAIIVVLCTLVMGSIFR
                     SLISDSSISFDISNFFSLNYLSFIGFLCISVLLLSYFLIIIRCIELVIRSMHRPLEFT
                     VWVAVISILALLGEYFIGHALQTDMVCTMGTYLLVDLPK"
BASE COUNT         2682 a         1935 c         1955 g         2865 t
ORIGIN      
        1 gaagcagtta gtgctatcca gaggaagtac atcattgagt acagtccaag tagcaccacc
       61 atcatctgat ctagagaagt tgatagttac aacaggagat gcgttagggc taacgaggta
      121 gatatcgtca gtacctgcag ggcaataagc gcgtggccac aagccatggt gcattcctga
      181 tgttggtaat tcagtccatg tagtaccgcc gatagaagca ttagcgaaaa ggcgctgatt
      241 tgttccatcg tgagcaataa ctacttcatg atccattaca gtaagcaact cagggaaacc
      301 agtgcgtaca gtttcaacac gagctgttgg tgcaggaccc catgaagctc catcattgtg
      361 attgtagaaa gaaccacgat cagcccaaga gccgtcggca acggtagatc ctgtccaaat
      421 ggcagagatt gcgccgtcgt cataaacacg tacacgatcg taagaagctg agttagacat
      481 cagcgggtaa ccggtaatac cgatagattc acctgtacga gcaccgctct ttgcatagaa
      541 agtgttggtg ttcagaggtg ttccattgag gtcggcatta acctgaggtt gatacggaac
      601 agctttggca ttcttgaatt tcgccatcat ttcagccttg ttgtgcggct tagccgtcac
      661 ggcagtctga gcgaaaagcg aaaccgagat aacgcctgct gctacggtta gtaataattt
      721 tctcatagca ttaaattttc gttagaaaga cattttgtgt aaagatataa agttaccgaa
      781 aaatcacata aaaaatgcga aatacatgca atttatagga attgacagtt atctgacatt
      841 gtcagtggag gcccgtgagt aggggggctc ctggtataga gcaaaaaaaa gagggctcca
      901 aaaggaaccc tccataaaaa aaattaagct gcttgcttag tttgtagcac catttgctgg
      961 cgctgcagga gcagcaactt tttttgcagc gggacgaccg cgttttccgg cttttttctt
     1021 aggagcggct tttttagcag cttttttagg agcggctttc ttagcagctt ttttagcggc
     1081 ttttttagga gcggcttttt tagcggcttt tttaggagca gcttttttag cagctttttt
     1141 aggagcagct tttttagcag ctttttttgg agcagctttt ttagctgctt tcttaccggc
     1201 aggacgtcca cgtttgccgg cggctttttt agttgttttt gccattggaa aaaatatttg
     1261 gttattgcaa aattatatat aaaatcatat tatcaaaaaa aaaagaaagt ttttttgttg
     1321 attttcacat gttttgcaca ttattgtata caacctttcc caatgtgatg tcatattcgc
     1381 acttcatggt tgataattgc tgctgaattt cttcatacag caattcaaaa aatcatgatt
     1441 gcttagctct ggaataaaaa atatagtcgg tatgtggcaa atcactcacg ccttgcatac
     1501 gcaaataatt gatgatctgt ccacgatgat aactgccatg attcatacaa tgtagtatga
     1561 tctctccatt gcgcgtttga tattcttgcc cattcaacgc cttgtaaaaa cacatgtcgc
     1621 cgaaaaaatc ttcaggcctg gaggaaacaa aacgagcgaa atcttcactc tgctgaatga
     1681 aatataaatc gtatccggca aattcatcgt catacatcgc acttggccaa tcgctgggtg
     1741 aagatccttg taaccgcgac aaccatatca cctgcgcatc ccaaatatga tataaggttt
     1801 ttctgatggt gggaaaactg cctcccatat gcttgtcgat gatctcagga gatacactgc
     1861 tgagtgtttg tatgatagcg gtgttcgccc atacattgta atcacagtaa ctatttaaaa
     1921 gctctttcat atatgcaggt taaaaccgaa agatagtaaa ctgatgtgtc gtgataatgc
     1981 acatcctgtg tattacggta agtattttga ggtcttgact tctatttaac agaatttacc
     2041 actgtgcaac ctatcaaacg atcataatcc ttgtcaaaca tatgcaggta aaccagcacc
     2101 acatagtcgt tttccgattc ataggtattg ccttctgtat aatcaagcgc atagtatgat
     2161 gctccctgcg gaagtagggc atactgataa ttataatatc cttgtttcaa atacgccttc
     2221 gcctcatatt gttgccggtc gtaattatat gtcataggta ttgcctgcaa agtggtatcg
     2281 ccctgcatgc cttcgcctat tacaaaaaca ttacctgacg taatcacata gggatattgg
     2341 agggtgaaat gtatccaggc ataatcgccc tctttgttta catcatcaga taactcagct
     2401 tgtatcacat attgcccgtt cgcatccttt tcataagtgt atcgctggta tactctgctt
     2461 tcatcaacat tcacatacac atgatattgt ccgtcctgtt gctgtatttt agtaatccgg
     2521 tcggtttgaa atttgagtgt tttggtatca aagcgacgat attcctttcc acccggaaat
     2581 acgagatcgt cgatatcata catcaacata tcattggtaa ccatccgtgg tttcaaatta
     2641 taggaggcat tatcaaagcg cccattttgt aataccgtta ctcgaatgtc gtcaaaggca
     2701 tcatgcacat cgatgttgcg gacatcaaca ttgaaattga tctcctgaaa ctcattgcga
     2761 taaggaatca gattcggacg catcactttg gcatcgatag cggcttcttg ttcccacaca
     2821 aaaaaccgct ttgttaatat tggtgtatcc ctatcctcat ccttccatac ctgcacaata
     2881 taattcccgc tcaacctgaa ttgcacatcc tcattaggaa acgatacggc ataatgtgta
     2941 tatcgctgca aggtaccaaa gctgaattga taatcgctga tgcgactttc atcaaatcca
     3001 tcaatgatat caaatgaggt cagtccactc ggttcccact gctgatcgca atgtgtgaaa
     3061 gtataatagt aatctttcac tcctccgtga aggtcatcaa aactgagtgt cagaaattga
     3121 ccatcagtat tcaaagggag tatcggatat gcaagctcaa gagtgctata ttgcaattgc
     3181 acagttctga tcatcggatc ggataccatt tctaccaacc cttttttaac ttcaggtatc
     3241 atcgtgctgt cagactgtgc cgatacacct atatatatag gtgtcgacaa cagaatgcaa
     3301 acaaccttca ttcggcagga gttaaaaaca gatggcatgc tatgctatta ttacaattag
     3361 gatgccaaga taattagcga cctttgtatt ttctaaaatc ggcatatgca aacctcaaaa
     3421 gaagccaaac tggaggcatt ctcacgttta ctggatatta tggatgactt gcgcagtcag
     3481 tgtccatggg ataaaaagca aaccattcat tctttgcgca accttaccat agaggaaacc
     3541 tatgagttgg cagatgcaat caccggaaat gattggaagg gaattaagga ggaactggga
     3601 gatattcttt tgcatatgat cttctacagc aagataggtg aagagcagga acaattttcg
     3661 attgctgatg tattacatgc tcaatgtgaa aaactcatac accgacaccc tcatatttat
     3721 ggcgatgtaa aggtgcagga tgaagatgaa gtgaagcgca actgggagca attgaaatta
     3781 aaggagggta aacgttctgt tttgcaggga gtgccaaacg gacttccggc ccttatcaaa
     3841 gcatatcgga ttcaggataa ggcggggcaa gttaattttg aatgggacca tatcgatcag
     3901 gtatgggtaa aggtgcggga ggagatgcag gaactacagg aagccaaaga tccggatcat
     3961 gttgaacagg aatttggcga tttgatgttt gcgctgataa acttatcgcg ttacctgaag
     4021 atagatccgg agaatgcact tgaacgcaca aataaaaagt tcatcagcag gtttagttat
     4081 atagaagagc aggctgccgg catgcaaaag aatttgcgtg atatgtcgtt ggcggaaatg
     4141 gatgcacttt gggataaggc taaagcaaaa gagcgcgaag cataattgcc tttcaattca
     4201 attaaccatt agctttttgt atcccatgaa ttccatcaaa ttttccggca gcatttcatt
     4261 gatttgccgg agttcttgag gatttatctt tttgatttgg tcaccactga gatgtgcaat
     4321 ttttttctcg ttgttaaatc ttctgctggc ataaacttta ttggtacttt gcactttttc
     4381 caactccggc cattccacta cttgagcaat ctgtttcatc accattgcag gatcggttag
     4441 cagatcatca tatcgcacaa ataagatatt ctttgcttca cgggaatagt tcatccacat
     4501 cttatagaat aatacatact ccataatata atgatgctga tgagccggcc agttgttttt
     4561 gatactccat ttattataac ttcccaacca actataggga tcttttgaca ctattatata
     4621 taggtcaggt gcctgaaacg gtaatgcagc ttcgaagtct ttcaatgttg gaaccaacaa
     4681 agtatttgcg aactgtggct caggaatgat ctctttttcc ttataaagtc tgaagtgttt
     4741 atgcgtaatc tcgttgcgtt gggttccgtt aagaaggagg ccatcgggat aatttaattg
     4801 caacagtgtc tccatatagt tggtgccgct tcgctgtaag ccatataaca gtatgccttt
     4861 tgccggaagc tttttcctga agatattgcg cagccaactc atgaagcacg aagatacaaa
     4921 acaaaaaaag cacccctgag gatgctttct tgtattgagg gtgattggat attgcttaaa
     4981 aagtttttgt cactttcatc atataagtac taccgatctt tggagcacca acaaaatatt
     5041 gctgctcatg attatataag ttggtgatga gcattgttat ctgagtgtct tttataaaat
     5101 ccggtgtata gttcaacgtt aggtccatat catgtatctc ttcaacagtg ccaacatatg
     5161 ctccggagtt tgcaggataa ctgctttgcc agcgccaggt gagtccaaca ttgaggtcgg
     5221 ccttcttgat atcataactt ccgcgaacac caactttatt tttcggagca ttgagcgcta
     5281 catacccgag ctgagcgcct tcaagttcaa cagaatcttt atcaacccag ctataggaga
     5341 attgcatggt aaggtcgtca ttcacatacc agctcacacc gatatcggta cctaaaactg
     5401 caatatcacc aaggttaaca taagtaacat atttggcacc atcaggatat tccattggcg
     5461 aaatagttcc cacaggtaat ccggcacctg ccgcaagaac gaggttgatg aattcatcta
     5521 caccggtccc gttattattt ccaccgtatg caggatcatc atcgagtgta gcggtaacaa
     5581 gacttgcaag aaactcgttt gatccggttg tgtcgtaata attttctgta atgataggtg
     5641 ctacataagc tgccagttct gcaccatcaa taaatacgtt cggcgtgata tcggtaagcg
     5701 gactcacata atcctgcatc atattataat atacatcaac ggtagcaaat actttatcat
     5761 taaacatact gcctttccag cctacttcga tagtattagt tgcagaattg agcatactgc
     5821 tgtagtcagt gatattggtt ggatctattg tttcaaattc ttcagttgtc aggttcaact
     5881 gcgcaatatt gttggaagta actggaattt cgggaggaag ccaagctacg agtgcagcac
     5941 ctaaacccgg aatggtagca tcaaatgcat cttctaataa tcctaaagtc aaatcccata
     6001 taacattatt aagttcgtaa ctgttatcaa ggctgaaata atgatcagat gcataaccta
     6061 aataagcaga gtagggtgaa gtatattgtg gtaaaccaaa atcatcataa gaataatgga
     6121 atccgtcacg attgcctatt gcatacaatg gcatattagg cacgtatgat gaagcaagat
     6181 atccttgaaa caaacctgca taacctgtca aatcgcctaa ttgcaatata tcaaggttga
     6241 ggttgttgct gcttggagca ctgaaggaac ggttgtaagt aagacggatg gtgtttttat
     6301 tgtttgcctt atacactaat gctgcgcgag gagaaaggaa aggattgttt acgaaattat
     6361 gataatcata tctcaatgca cctactaaac tccatttagg atttaccatg taatcaccct
     6421 gtgcataggc gccgatttca atgatattat catcatcttc attttcgcca ttgatggtat
     6481 tttcagtatc aggattggtc ataatggcgt ctaaaccata taccagattt aattttttat
     6541 ccatccagtc attggcatgt tgaatctgac caacatattg cttcgaatgg tctatgatga
     6601 ggtcgccggt tctaaaaaga taggtgtctc cggcatcgct gccattaaca aatccttgaa
     6661 caaaccaatc gttatggatg tatcgcaatt gcgcataata atatttccat cccaccgctt
     6721 gtccggcacc gatacctgtt aattcaatac catcattttg tgtcattcct cccgaaagaa
     6781 caaattctgc tttgtttttc aggcgatagt cgaagcgcag atcaacgcca attttttcaa
     6841 tgtgactgtc gcgtgtatta tcaatagtat cgccaacgtt ttgacgacct tcagaagttt
     6901 ggtagcctaa ttgtacttgt gcaggttcaa atggatcatc gtaaagccag tccattccgt
     6961 tgaggtaggt gaaaaggata cgatatccga attggtcatt cacttttcct gagtgacgga
     7021 agccggcagt ataaaatgtt ctttcgccga tattgtcgta atcgaacaat ggcgtataat
     7081 caccatatac aagtcctgta gccggatcaa tagggttggc gatcatgatg gtatcgctta
     7141 tcatgcttcg aaatcctaaa cccagagagg cagaggtcat gtatttcttt tcctgactta
     7201 gaggcgattt tgtgatcata tgaaatacgc cattcgaaca gttcggacca tataaagcag
     7261 atgcaggtcc gcggagcact tcgatacgtt cgaggtcaag gttgtcggta ggtataaatg
     7321 ttgcaacatt ggctctaaga gaaggcacgg cagcatatct attatctacc attgtcaata
     7381 aagcaccact gaaaatatta ttgaatccgc gaacaaccac attggtttgc actaaaccgg
     7441 tattgatgat atctactccg ggaacaccct tgatattttg aattggcgtg atggcaaccg
     7501 tatttttaat ttgctcagga ctgataagcg tgatggaggc aggtgcatcc agtatctttt
     7561 cctgtcgacg tgaggcgctg acaacaatgg catcgagatt ataatcttca ctagtcagca
     7621 ttacctcata ttttgctgat gtggtaagtg caacttcaat ttctgcatag cctacagatc
     7681 tgaccataag ggcttgtgta ttagcaggaa cctcaagttt aaattcacca ttaatatcgg
     7741 ttgctgttcc caaagacctg tcttctttgg tcattacact ggcgcccgga attgtttcgc
     7801 ctgttgcagc atccttcaca acgcccgtta ctgttcgatt ttgtccaaaa gcctgagcgc
     7861 cgagcagcat aataaacata attaagaggg tacaaacgtt tctcatgcaa agtggtttta
     7921 aaggtaatgt gggtttggtt gctaacttta cgcctgtaaa tggcctcaaa gtgcacaaaa
     7981 acaggctatt gcgttatcaa aattgggtta aaagttggag aaagctataa tattacctgc
     8041 aaataacaaa attcgaggct gttgaaaatc aggcgttttc atattatcct gccaatagtt
     8101 ttcgctttat gcgcgataac agcttatgtt attgccggca gagctgaacg cagtcaaagc
     8161 cttgaggcta cagccaaaca tattgagcac gattatcaaa ccctggaacg agagatcatt
     8221 gctgttattt ctgatgaaat gatcatcagt aaccttataa cagatagtta cacctttgaa
     8281 gagtttagtc gcctcgctga acgtccgtat ggaattgttg tttatcagaa tgacagtgct
     8341 ctcatttgga caaacaattt catcactccc ataaaggcac aggtcccctt taatgatgcg
     8401 ccattattga tttctgaatc gaatggcgag tatattatat ttcaacatga tttccccggc
     8461 gatgtacatg tgattggatt tctgccatta caatttgaat atggcattcg gaataagtat
     8521 ctcgaccatg taaaatctcc gggcatgcat attccggatt ttgttagaat taatccgcgt
     8581 gccgaaggtg catccactcc tgttatgtcg aatagcggga atgccgtgtt ttatgtcagc
     8641 aatgatggcc tggaaactgc tgcaactgta tatccgcaaa gtatgtttgt catgctgatc
     8701 ttatcggctt tgttctggtt gatgtgcatg tatcaacttg caagatggat agcattcaaa
     8761 ttcggacgat tacccgcagt tggggcattg ttgattttgc tgtcgctgag ttatatactt
     8821 ttacgcgacc tcttttatga taatgtactg agcgccttac cgcttttcga ccctcaattt
     8881 tatggctcac cgggtattgc aggatctacc ggtgacttgc tgataaaaac tgtctggcta
     8941 ttttgtttcg cactcttttt ctctcgattt ttcttcatta aacaactgcg ctctgtttgg
     9001 ctgaaggcaa cactcgctat cattgtggtg ttgtgtacat tggtgatggg aagcattttc
     9061 aggagtttaa tttcagactc ttctatttct ttcgatatca gcaatttctt ttcgctcaac
     9121 tacttgagct ttatcggatt cctttgtatt tcagtgcttt tactcagcta cttcttaatt
     9181 attattagat gtatagaact agtaatacga agtatgcatc gaccactgga atttactgtg
     9241 tgggttgcag tgattagtat actcgcttta ttaggtgaat attttatcgg tcacgccctg
     9301 caaactgata tggtgtgtac catgggtact tatcttttag tggatctacc gaaataaacc
     9361 tccctgtttt cgggtcagaa attctaaacc cataatcctg cacatcccat tcctcattct
     9421 ccttcccatt aaatcca
//
LOCUS       DAOQHY010000127         9359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_3726_length_9359_cov_5.457008, whole genome shotgun sequence.
ACCESSION   DAOQHY010000127 DAOQHY010000000
VERSION     DAOQHY010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9359
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3726_length_9359_cov_5.457008"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1382
                     /locus_tag="PLL28_10555"
     CDS             114..1382
                     /locus_tag="PLL28_10555"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNF69809.1"
                     /translation="MHVDVLLGLQWGDEGKGKVVDYLAEQYNVVARFQGGPNAGHTLI
                     LNNGEKFVLHTIPSGIFLNNCLNVVGNGVVIDPITFRMEVQKLEKAGVDVTHRLVIAR
                     KAHLILPTHKMLDAASEASKGQDKIGSTLKGIGPAYMDKTGRNGLRVGDIESPDFLKK
                     YNTLRDKHFQLLRIYGEHHNEVPNEQEWMDAIAFLKQLPFVDAEYLVDEAMHQGKTIL
                     AEGAQGSMLDVDHGTYPFVTSSNTITAGVCTGLGVAPQRVKEVIGISKAYCTRVGGGP
                     FPTELFDADGEELRKIGNEFGATTGRPRRCGWIDIPALKYTIMLNGVTQLAITKVDVL
                     NTFTKIKACVAYKINGVITDRVPYDIVDAVIEPVYEEFPGWNCSLDGCKSKADLPEAL
                     QNYLQFLEWHLKTRITMLSAGPERDKLILL"
     gene            1434..2741
                     /locus_tag="PLL28_10560"
     CDS             1434..2741
                     /locus_tag="PLL28_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HNF69810.1"
                     /translation="MRTSAQFQVGEDFRSRLSEFAGRYEEYALLDSCGNTEYGANTFD
                     YLLACAAVAVIVHTEGESVSAELKAAIHEHGDYLFGYLGYELNALQEPVFSSPQQLFQ
                     LPAMRFFVPGILVMVKDGTATISIHESTCDTAENVFRTISEISEENLGNFLHNPSPIT
                     LKPLVSASDYLSTVDKIRQHIIDGDIYEMNYCQPFVANDVNINPTNLFNRLCSIAKAP
                     FSVLYRFGEQYLICGSPERFFSYNGETMCSMPIKGTRRRGGDAVSDKRLRDELITSEK
                     DRAEHVMIVDLVRNDLTPYAIPGSITVDNLFGIYGFEQVWQMVSTIVARLRPDAHAVD
                     ALLQAFPMGSMTGAPKIRAMQLAAQYEPFQRGMYSGTFGYFTPDGHCDFNVVIRSMLY
                     NSEQQILAAFAGGAIVYDSVAEKELEECLLKLSAIRRVLSDLP"
     gene            2780..3370
                     /locus_tag="PLL28_10565"
     CDS             2780..3370
                     /locus_tag="PLL28_10565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69811.1"
                     /translation="MRNLILLLSFFTWQQVKAQGPFRDVTAAQFGDSILLNWTLTAGN
                     TCFDMHLQRSDGTTPFTTVFSVPGICGGTQDQYYSFVDYEDLKSGITYQYRLTASNDA
                     FISDSVLITYIDAGDRTLFVYPNPTKDHLELTIDNSLQPSFLIEVFDISGKRYSLSTY
                     PSNLVTLPTSDLSPGIYNLIVTTRDGQKFASDFIVQ"
     gene            complement(3357..4637)
                     /gene="tyrS"
                     /locus_tag="PLL28_10570"
     CDS             complement(3357..4637)
                     /gene="tyrS"
                     /locus_tag="PLL28_10570"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNF69812.1"
                     /translation="MKNFVEELRWRGMLFDMMPGTEEFLNKGMATGYIGFDPTADSLG
                     VGNMVQIMTLLHFQECGHKPIALVGGATGMVGDPSGKSAERNLLDEETLQKNIAAQKK
                     QIEKFLKFGNEGNAAEMVNNYDWMKDFSFLDFLREAGKYITVSYMMAKDSVQTRLERG
                     ISFTEFSYQLLQGYDFYYLWKNRGVMLQMGGSDQWGNITTGNEMIRRKGGGEAYAICT
                     PLIKKADGTKFGKTEGGNVWLDAKRTSPYKFYQFWLNASDTDAETYIKIFTLLDKTTI
                     DNLIEEHRAQPHLRLLQKTLAKDITIRVHSEEDYNNALAASDILFGKSTKADFEHLTE
                     ELLLDVLEGVPQFSIAANDLSAGLTANQILTEKTQVVSSKGEFKRALQENSLSINKEK
                     LSDGEMIITQADLIKGKFLLVQRGKKNYYLIIAQ"
     gene            4793..5467
                     /locus_tag="PLL28_10575"
     CDS             4793..5467
                     /locus_tag="PLL28_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69813.1"
                     /translation="MNFLSHYYHELPCNDAYFVGGLILPDILSNYSYRAGRVVKLHPW
                     RLTEPKTAIQQSLTSGVKRHYAVDAAFHDSDYFTIQTRWIEDNLLSKPFDCFPRRKYA
                     IAHVMLEILLDRSLMVRFPDLTPGFYAKLEEIDEHAIAELMSNNGQEKDAVGVRAHFN
                     EFRRAAFLYDYLNDERLNALLARINRRLGNPLLSASDKQNLTTAIYDIEKAFLGEKFP
                     TFRTDS"
     gene            5514..6569
                     /locus_tag="PLL28_10580"
     CDS             5514..6569
                     /locus_tag="PLL28_10580"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HNF69814.1"
                     /translation="MSAAHTQTTAPKYSNEFLSIGVSAQALGMSNSVVAGVNDVTSAY
                     WNPAGLMLVEPDIQLGAMHAEYFAGIAKYDYGSVAIPLREKQAAIGFSLIRFGVDDIP
                     NTLYLVEPDGSINYDNITAFSVADYAFLGTYATKLKWQNLRIGGSAKVVHRQAGEFAT
                     AWGFGFDAGAQMDYKKWKFGVQLRDITSTFNAWSFSFTEEEKEVLTATGNSIPTNSLE
                     ITTPKMILGAAYDFSLGTKFGLRTELDADITTDGRRNVLLSANPISVDPHLGVEADYQ
                     DFIYLRAGIGNVQRYIPDDLTEEAWTLQPNMGLGIIIGPMKIDYAYSNIGKQAEVLYS
                     HVFSLRLDINKNIKSAK"
     gene            6595..>9359
                     /locus_tag="PLL28_10585"
     CDS             6595..>9359
                     /locus_tag="PLL28_10585"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNF69815.1"
                     /translation="MRRYLLTLTTIFFFIQAKAQTYGNEWIAYDQQYLKCYVAADGIY
                     RIDYNTLNTSLAGIGVSLSDIDPRHFQLFNQGEEQYIFIAGEADGSFDPADFIEFLGK
                     RNDGTFDTGLFDSAQFQLQTFESIISDTACYFLTWNDLFDNNRMAPAINDLAGAPSPA
                     PYFSAHSRIVFGSGYGSANFNAGPSYQNVYSSKYMNGEGFTSPKYNLSTYNVTVNTPN
                     RYAGVDGFAPTLSTIAIGSNEAEHHVVIKINASTMLDTTFSNYGVVRKTFLMPTITDA
                     NTVTFVSGPTTTDYQRYSFIDINYPRIFDFDNASRFRVDLPAIPGPTTYLEFNDMDEK
                     GTPVLLYDLAEKRRMIGITEADITKFHIPYPASLHKLYVTSQDPSDIKSITTLSPVTF
                     INYGDVANQGDFLIISHPSLFDDGTGTNYVAAYRDYRNSIDGGSYDARIANIDMLYDE
                     FSYGIRKHPLAIRNYILYAVDHYTDKPKYVFLIGKSFSYDVTRSNGTPEFYEDLVPTF
                     GHPGADALLAARPGSVVQTIPIGRIAAKTADEVRIYLDKVIAFEEAQHDNTQTVANKL
                     WMKNVLHFAGGLNEYEQALFNNYLNQYSYYIEDTLFGGNVRQFNKLSTDPIFYSESEY
                     IDSLINAGISLATFFGHSSTGSFDFNIGEPEDLNNAGKYFCVYGNGCNTAAIHGESLT
                     LGERYIFAEEKGAVAFIAASNFSLASSLNTYANMFYQEFSNYAYDLPLGDVLMRTTDT
                     LWPTGNIYQQLTLEHTTLQGDPSLRLNTHPQPDYDIEAPLVSFQPDIITPGIDTFYLE
                     LIVSNLGMAIDSSYFVEVKRTLPDGSEQTFFERFPSTYYQDTLHIPFATGGPDGVGIN
                     NFNIHVDKLNEIPEIDELNNVLGTSVTIISDDAIPIYPVEFSIMNHVPEYFAASTSYV
                     FAEEKLYL"
BASE COUNT         2575 a         1981 c         2018 g         2785 t
ORIGIN      
        1 cactcaccac tcaccactca ccactcacca ctcaccactc accactcacc actcgccact
       61 taccacttgt caatgcccct aagcttccct aacttcgcac tttaatacgc gatatgcacg
      121 tagatgtttt actaggcctg cagtggggcg atgaaggaaa gggaaaagtt gtcgactatc
      181 tggcagagca atacaatgtg gtagcccgtt tccagggcgg tccgaatgca ggacatacac
      241 tgatccttaa caatggcgag aagttcgtgc tccataccat tccgtcaggg atatttttga
      301 ataattgtct gaatgtagtg ggaaatggag tggtaattga ccctattacc ttccgtatgg
      361 aggtacaaaa actggaaaaa gcgggcgtag atgtcactca caggctcgta attgcacgca
      421 aggcccattt gatactacct acgcataaga tgcttgatgc cgctagcgag gctagcaaag
      481 ggcaggataa gattggatca acgctcaagg gtatcggtcc ggcttacatg gacaaaaccg
      541 gtagaaacgg cttgagggtg ggtgatatcg aatcgcctga tttcttgaag aaatataata
      601 ccctgagaga caagcacttc cagctgttac gtatttatgg tgaacatcat aatgaggtgc
      661 caaatgagca ggaatggatg gatgcaatag cttttttgaa gcagttgccg tttgttgatg
      721 ccgagtattt ggtggatgag gctatgcatc agggaaagac catacttgcc gaaggtgcac
      781 aaggttctat gcttgatgta gaccacggca cttacccatt tgttacatcc agcaatacca
      841 taacggcggg cgtttgcacc ggcttaggtg ttgcccctca gcgcgtaaaa gaggtgatag
      901 gtatctctaa agcttattgc acacgcgtag gcggagggcc ttttccaacg gaattatttg
      961 atgccgacgg agaagaatta aggaaaatag ggaatgaatt cggcgctact accggtcgcc
     1021 cgcgtcgctg tggatggatt gatattccgg cgctgaaata taccatcatg ctcaatggtg
     1081 taacgcagct tgctatcaca aaagtggatg tgctgaatac cttcaccaag atcaaagcat
     1141 gtgtagcata caagatcaac ggcgtgatca ctgatcgcgt accttacgac attgttgatg
     1201 cggtgattga gcccgtttat gaagaattcc caggttggaa ttgttcgctt gacggctgta
     1261 aatcaaaagc agatttacca gaggcgttgc agaattattt gcagtttttg gaatggcatc
     1321 tgaaaacacg tatcaccatg ctatctgctg gtccggaacg cgataagctc attctgttat
     1381 aagcagtaat tattttttgc tgagcgatcg gcatcttact tttcaaaacg gcattgcgta
     1441 cgagtgcaca atttcaggtt ggggaagatt tcagatcccg actttcggag ttcgccggga
     1501 gatatgaaga atatgccctg ctcgatagct gcggaaatac ggagtacggt gctaatacct
     1561 tcgactatct tctggcttgt gctgcggttg cagtgattgt acataccgaa ggcgaaagcg
     1621 tttcggcgga actgaaggct gctatacatg aacatggcga ctatcttttc ggctacctgg
     1681 gttacgagtt aaatgccctg caagagcctg ttttttcctc cccccaacaa ttatttcaac
     1741 tgccggccat gcgctttttt gtgcccggta ttttggtcat ggtgaaagat ggtactgcaa
     1801 ctatttctat ccatgaaagc acctgtgata ctgctgaaaa tgtatttcga accatctccg
     1861 aaatttcaga agaaaactta ggcaattttt tacacaatcc ttctccaata acattaaagc
     1921 cgcttgtaag cgcatctgat tacttgtcaa cggtcgataa aatcagacaa catatcatcg
     1981 acggcgatat ttatgaaatg aattattgtc agccatttgt tgcaaacgat gtaaatatta
     2041 atccaaccaa tttattcaac cgcttatgtt caatcgccaa agctccattc tctgttttat
     2101 atcgatttgg tgagcaatat ttaatttgtg gaagtcccga aagatttttc agttataatg
     2161 gtgaaaccat gtgcagtatg cccatcaaag gcacgcgaag aaggggtgga gatgcagtga
     2221 gtgataagcg gttacgagat gaattgataa ctagtgagaa ggatagggca gagcatgtta
     2281 tgattgttga tctggtgcga aacgacctta cgccatatgc cataccggga agtataacgg
     2341 tagacaattt gttcggcatc tatggttttg agcaggtttg gcagatggtg agcacgattg
     2401 ttgcgcgttt gcgacctgat gcgcatgcgg tggatgcctt attgcaagca tttcctatgg
     2461 gtagcatgac aggggctccg aaaatcaggg ccatgcagct ggcggcgcaa tatgaacctt
     2521 ttcagcgggg gatgtattca ggtactttcg gatattttac tcccgatggt cattgcgatt
     2581 ttaatgttgt gatcaggagc atgctttata acagcgagca acaaatctta gcggccttcg
     2641 ccggtggtgc catcgtgtat gattcagtgg cagaaaaaga actggaagag tgtttgttga
     2701 aactttcagc catccggcgt gtgttgtcag atttgcctta acatactaaa atcaggctat
     2761 agacgtttta tacccttcga tgcgcaatct tatactgcta ttatcgtttt tcacatggca
     2821 acaggtaaag gctcaaggcc ctttccgcga tgtcaccgcc gctcaattcg gtgatagtat
     2881 tttattgaat tggacgctta ccgcaggaaa tacttgcttc gatatgcatc tgcaacggtc
     2941 tgacggcaca acgccattta caacggtatt ttctgttccc ggaatttgcg ggggtaccca
     3001 agatcaatat tatagttttg tggattacga agatttgaaa tcgggcatta cctatcaata
     3061 tcgactgact gcttccaatg atgcatttat cagtgatagt gttttgatca cctatattga
     3121 tgccggtgat cgcacattat ttgtatatcc gaatccaacg aaagatcatc tggagcttac
     3181 cattgacaat agcttgcagc cttcattttt aattgaagta ttcgatatat cgggaaagcg
     3241 ttacagcctg tctacttatc cgtcaaatct ggttactttg cctacatcag atttgagtcc
     3301 gggtatctac aatttgatcg ttaccacgcg cgatggacaa aaatttgctt ctgattttat
     3361 tgtgcaataa tgaggtagta atttttcttc ccgcgttgca ctaaaaggaa ttttcctttg
     3421 ataagatcag cttgtgtaat tatcatttca ccgtccgata atttttcctt gttgatactc
     3481 aatgaatttt cctgcaaggc gcgtttaaat tcacctttcg aggatactac ctgtgttttt
     3541 tcagtaagta tttgattggc tgtaagtccc gccgacaagt catttgcggc gattgagaat
     3601 tgcggaacac cttcgagaac gtctaacaat aattcttccg taagatgctc gaaatcggcc
     3661 ttcgtactct taccaaacaa tatatcacta gcagcaaggg cattattata atcctcttca
     3721 ctatgcacgc gaatggtgat atcttttgcg agtgtttttt gcaataaacg caaatgtggt
     3781 tgtgcgcgat gttcttctat cagattatcg atggtggttt tatcaagcaa agtaaatatc
     3841 ttgatataag tttccgcatc agtatcactg gcattcaacc aaaattgata aaatttatac
     3901 ggtgatgtac gttttgcatc aagccacaca ttgccacctt ccgtttttcc gaatttggtg
     3961 ccgtcggctt ttttgatcag tggagtacaa atagcatatg cttcaccacc accttttcga
     4021 cgaatcattt cattgcctgt tgttatattt ccccactggt cgctgccacc catttgcagc
     4081 attacaccgc gatttttcca gaggtaataa aaatcataac cctgcaacaa ctgataactg
     4141 aattccgtaa atgagattcc gcgttccaga cgtgtttgca cagaatcttt tgccatcata
     4201 tagctaacag taatatattt tcctgcttca cggagaaaat cgaggaatga aaaatctttc
     4261 atccaatcgt aattattcac catctccgca gcattgcctt cattgccgaa tttgagaaat
     4321 ttttctattt gctttttttg agcagcaata tttttttgaa gcgtttcttc gtccagcagg
     4381 ttgcgttcag cactttttcc cgacggatcg ccaaccatgc cggtagctcc accaacaagt
     4441 gcgatgggtt tatgtccaca ttcctgaaaa tgcaacaatg tcatgatctg caccatattg
     4501 cccacgccca atgaatcagc cgtcgggtca aaaccaatat accctgttgc cattccttta
     4561 tttaaaaact cctcagttcc gggcatcata tcgaatagca tcccacgcca gcgcaattct
     4621 tcaacaaaat ttttcatgca gcaaagatag gcgggatttg ttatttgact gaaaatgacc
     4681 catttgccgc ttgtactgtt tacttgtcat atttgaatca aacttgcacc caaaggctga
     4741 tatccaccgc gaggagcaaa cctttccggc tgtttttccg ttaaattgct aaatgaactt
     4801 cctttcgcat tattaccatg agttgccctg caatgatgcc tattttgtgg gaggattgat
     4861 attgccagat atcctgtcga attacagcta tcgggcggga cgggtggtaa aactgcatcc
     4921 atggcgattg accgagccca aaacagccat acagcagtcg ctcacatccg gagtcaaacg
     4981 tcattatgca gttgatgcag cctttcacga tagcgattac ttcaccatac aaacgcgatg
     5041 gattgaggat aacttgcttt caaagccttt cgactgcttt ccacgcagaa aatatgccat
     5101 tgcccacgtg atgctggaga tactgttgga ccgcagtctg atggtccgtt ttccggatct
     5161 gacacccgga ttttatgcta agttggaaga gattgatgag catgcaattg ccgaattaat
     5221 gtccaataac ggtcaggaaa aagatgccgt aggggtgagg gctcatttta acgaatttcg
     5281 ccgcgctgct ttcttatacg attacctgaa tgacgagcgt ttaaacgcct tgttggcacg
     5341 tatcaacaga aggcttggaa atcccctgct ttcagcctcc gacaaacaaa accttacaac
     5401 agcaatttat gacattgaaa aggcgttcct cggcgaaaaa ttccctacat ttcggacgga
     5461 ttcgtgacct gaccatgaga tgtaccttcc ttttgctgct cagtattggc atcgtgtcgg
     5521 cagcacacac acaaacaacg gcgccgaaat acagcaatga atttttatcc attggtgtgt
     5581 ctgctcaggc attgggtatg agcaatagtg tggttgccgg tgtcaatgat gttacctcag
     5641 cgtactggaa tccggcaggt cttatgcttg ttgaacctga tattcaatta ggtgccatgc
     5701 atgccgaata tttcgccggt attgcaaaat acgattatgg ttctgtagct attccactgc
     5761 gagaaaaaca ggcggccatc ggattttcac taattcgctt tggtgtggat gacatcccta
     5821 ataccctgta tcttgtagaa cccgatggta gtattaacta tgataatatc actgcctttt
     5881 ctgttgctga ttatgctttc ctcggcacct atgccaccaa actgaaatgg caaaatctgc
     5941 gcatcggcgg atctgcaaaa gtggtgcatc gccaggcagg agaatttgct accgcctggg
     6001 gtttcggctt tgatgccggt gcacaaatgg attataaaaa atggaaattc ggcgtgcaat
     6061 tgcgtgatat cacatcaaca tttaatgcct ggtcgttttc atttacggaa gaagaaaaag
     6121 aggtgcttac agcaaccgga aattccattc ctacaaattc gttggagatt acaacgccga
     6181 aaatgatttt gggtgcagca tatgattttt cactcggcac aaaatttggt ctgcgcacag
     6241 aacttgatgc cgatattaca acagatggca gaagaaatgt tttgttaagc gcaaatccga
     6301 taagtgttga tccacatctg ggcgttgaag cagattatca ggattttatt tatttgcgcg
     6361 ctggaattgg caatgtgcaa cgctatattc cggatgattt aactgaagag gcatggactc
     6421 ttcagccgaa tatgggatta ggcattatca tcggtccgat gaagattgat tatgcctatt
     6481 cgaatatagg caaacaagca gaggtgttat atagtcatgt gttcagcctt agactggata
     6541 tcaataaaaa tattaagtct gccaaataat tttttcatgt aacctgcaac ctgcatgagg
     6601 agatatttac taacgctaac taccatattc tttttcattc aggccaaagc acaaacttat
     6661 ggcaatgaat ggatagctta tgatcagcag tatctcaaat gctatgtagc cgccgatggt
     6721 atttatcgca tcgactataa tacactgaac acttcgttag caggaattgg tgtttcgctt
     6781 tccgatattg atcccaggca ttttcaattg tttaatcagg gcgaggagca atatattttt
     6841 attgcaggtg aagctgatgg ttcatttgat ccggccgact ttattgaatt tcttggcaag
     6901 cgcaatgacg gaacatttga taccggatta tttgattcgg cacaatttca gttgcaaaca
     6961 tttgaaagta ttatcagtga tacagcttgt tatttcctga catggaacga cctgtttgac
     7021 aataaccgaa tggcaccggc tatcaatgat cttgcaggag cgccatcgcc ggcaccgtat
     7081 ttttcggcgc attccagaat tgttttcgga agtggatacg ggtctgcaaa ttttaatgca
     7141 ggaccttcat atcaaaatgt ttacagctct aaatatatga atggtgaagg atttacttca
     7201 ccaaagtata atttatctac ctacaacgtt actgtcaata cgccaaacag atatgctggt
     7261 gtcgatggat ttgcaccaac actcagcacc attgctatcg gttctaatga agctgagcac
     7321 catgtggtta ttaagatcaa tgcatctacc atgctggata ccactttcag taattatggt
     7381 gttgtccgca aaacattttt aatgcccact atcaccgatg ccaatacagt gacatttgta
     7441 agcggaccta ctactacaga ttatcaacga tattccttta ttgatatcaa ttatccgaga
     7501 atttttgatt ttgacaatgc ttcgcgtttt agagtggacc tccctgcaat tcccggacct
     7561 actacatatc ttgagtttaa tgatatggat gaaaaaggaa cacctgtttt gctttatgac
     7621 ctggccgaga agcgcagaat gattggtatt actgaagcag atattacaaa atttcatatc
     7681 ccttaccccg catcgctcca taaattatat gttaccagtc aggatccaag cgatattaaa
     7741 tctattacca cattatcacc tgttactttc atcaattatg gtgatgtcgc caatcaggga
     7801 gattttttaa ttattagtca cccttccctt ttcgatgacg gcaccggaac aaattatgtc
     7861 gcagcttacc gcgattacag aaattcaatt gacggtggaa gttatgatgc gcgcattgcc
     7921 aatattgata tgttgtatga tgaattcagc tatggcatca gaaaacatcc cttggctatt
     7981 cgcaattaca tcctttatgc tgtagatcat tatacagaca aaccaaaata tgtatttctc
     8041 atcggcaaat cgttttctta tgatgtaacg cgctctaacg gtactccgga gttttatgaa
     8101 gatttagttc ctactttcgg tcacccggga gctgatgcgc tacttgctgc caggcctggc
     8161 tcagttgttc aaacaatacc tattggaagg atagccgcaa aaactgcaga tgaagtgcgt
     8221 atttatcttg ataaggtaat agcatttgaa gaagcgcaac acgacaatac gcaaacggtt
     8281 gccaataaac tgtggatgaa aaatgtgttg cacttcgccg gcggtttgaa tgagtatgaa
     8341 caagccttgt ttaataatta tttgaatcag tattcctatt atattgaaga cactttattt
     8401 ggcggaaatg tgcgtcagtt caacaagcta agtaccgatc ctattttcta cagcgaaagt
     8461 gaatatattg attcgttgat caatgccgga atttcattgg caacattttt cggacattcc
     8521 tcaaccggaa gtttcgattt taatattggt gaacctgaag atctgaataa tgcagggaaa
     8581 tatttctgtg tatacggaaa tggatgcaat acagcagcaa ttcatggtga gagcctgaca
     8641 cttggtgaac gatatatttt cgcagaagag aaaggtgccg ttgcatttat cgccgcttct
     8701 aatttttcgc tggcaagtag tttgaatacc tatgcaaata tgttttacca ggagttttca
     8761 aactatgcct atgacttgcc tttgggagat gttttgatga ggactacgga tacattatgg
     8821 cctacaggaa acatttacca gcaactcacc ttagaacata ccacccttca gggagatccg
     8881 tcattgcgtt taaatacgca tccgcagcct gattatgata ttgaagcacc attagtaagc
     8941 ttccagcccg atattatcac tccgggtatt gatactttct atctcgaact tattgtgagc
     9001 aaccttggga tggctatcga ctcttcctat tttgttgaag taaaacgcac cttacctgat
     9061 ggtagtgagc aaacattttt tgagcgattc ccatcaacat attaccagga tactttgcat
     9121 attccctttg ccaccggcgg acctgatggt gtgggtataa ataattttaa tatacatgtc
     9181 gataagctga atgaaattcc ggaaattgac gaattaaata atgtactggg cacttcggtg
     9241 acaattatat ctgatgatgc tattccaatt tatcctgtgg aatttagtat catgaatcat
     9301 gtgcccgagt attttgcggc atcgacttcc tatgtgttcg ctgaggagaa attgtatct
//
LOCUS       DAOQHY010000128         9224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_3795_length_9224_cov_5.178864, whole genome shotgun sequence.
ACCESSION   DAOQHY010000128 DAOQHY010000000
VERSION     DAOQHY010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9224
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3795_length_9224_cov_5.178864"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..276
                     /locus_tag="PLL28_10590"
     tRNA            204..276
                     /locus_tag="PLL28_10590"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Gln,seq:ttg)
     gene            411..1484
                     /locus_tag="PLL28_10595"
     CDS             411..1484
                     /locus_tag="PLL28_10595"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HNF69816.1"
                     /translation="MSYNNIVSLLGDKAEYLLNHESKTISRDSLHLPGADFVDRVWIP
                     SDRNPQVLRNMEAMFGHGRLANTGYLSILPVDQGIEHSAGASFAKNPIYFDPENIVKL
                     AIEGGCNAVATTYGALGAISRKYAHRIPFIVKINHNEFLTYPNKFDQVMFTSIKASWE
                     LGAAAVGATIYFGSPESTRQLQEVSEAFQYAHELGMSTILWCYLRNPAFKQADKDYHV
                     SADLTGQANHLGVTIEADIIKQKLPENNGGYLALNSKESPYGKNDKRIYEELTSEHPI
                     DLCRYQVANCFMGRAGLINSGGASSGAADLTEAVTTAVINKRAGGMGLISGRKAFQRP
                     MKEGVEILNAIQDVYLTKEVTIA"
     gene            1558..2439
                     /gene="accD"
                     /locus_tag="PLL28_10600"
     CDS             1558..2439
                     /gene="accD"
                     /locus_tag="PLL28_10600"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNF69817.1"
                     /translation="MAEQKPDKSSWFKRVRKGIETSTEEKLDTPEGVWWQCPECKKTF
                     TAIEYETNLWCCPNCAYHGRIGSKQYFSILFDEGKHSLICEEVRAKDALNFKDLKPYS
                     DRLKDAWKKSEYTDAITVGTGPIDGHDAVIACMNFEFIGGSMGSVVGERISRAIDYCV
                     AHNQPLLIISKSGGARMMESAYSLMQMAKTSAKLTLLEKHRLPYISICTDPTTGGVTA
                     SYAMLGDVNIAEPKALIGFAGPRVVKETIKKDLPKGFQSAEFVLETGFLDLILERKLL
                     KSNIARLLKHFRNEASA"
     gene            2707..2979
                     /gene="rpsO"
                     /locus_tag="PLL28_10605"
     CDS             2707..2979
                     /gene="rpsO"
                     /locus_tag="PLL28_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNF69818.1"
                     /translation="MAVLTAEKKKEIFKDFGGNETNTGSVEAQIALFTNRINHLTDHL
                     KANKKDFATKRSLLMLVGKRRRFLNYLMKKDIKAYRALIEKLNIRR"
     gene            3188..5332
                     /gene="pnp"
                     /locus_tag="PLL28_10610"
     CDS             3188..5332
                     /gene="pnp"
                     /locus_tag="PLL28_10610"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNF69819.1"
                     /translation="MKPNPITIEVPLSEGRVVTIETGKLAKQADGSVVVRCGDTMLLA
                     TVVSAKEAKDGVDFMPLTVDYRENYAAAGRFPGGFLKREARPSEYEILISRLIDRAMR
                     PMFPDDYHADTQVLVTLISLDKEILPDALAGLSAAAAIAVSDIPFNGPISEVRVGRID
                     GKFVINPTRTELERSDIDMIVAGSIKDVVMVEGEMKEVKEEDMLEAIRVAHQVIQEQC
                     KKLNELTTLVGKETKRAYSHEVNDQDLKDRMFRELYDKVSAVAHMPSAKEVRGDAFKL
                     IKEEFKATFSEEELAEKSGMIDRYYKDVEKHAMREMILTDRQRLDGRRMDEVRPIWAE
                     VDYLPSAHGSAVFTRGETQSLTTVTLGAKMDEQLIDGVVHEGTSKFLLHYNFPPFSTG
                     EAKPMRGLGRREVGHGNLAMRAIKWSLPEENAYTIRVVSDILESNGSSSMATVCAGSL
                     ALMDAGVQVKKPVAGIAMGLVSEANMVRYGILSDILGDEDHLGDMDFKVAGTVDGITA
                     TQMDIKVDGLSFGVVKEALEQARAGRLHILNEMSKAIQGPRPDLKPHAPRITTIQIPR
                     DYIGAVIGPGGKIIQEMQRESGAEISIEEIGDFGHVVISSPNLESIEKALNRIRAIVA
                     IPEVGEVYEATVKSIMPYGAFVEFLPGKEGLLHISEVDWKRLESLEGILNKGDKLQVK
                     LLEVDQKTGKFKLSRKVLLERPPRPQANNEQH"
     gene            5505..6371
                     /locus_tag="PLL28_10615"
     CDS             5505..6371
                     /locus_tag="PLL28_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HNF69820.1"
                     /translation="MRQLKITASITSRENDSLEKYLAEIGKIDMITPEEEAVLAKRIR
                     EGDTVALDTLTKANLRFVVSVAKQYQGHGLTLSDLINEGNVGLIKAAKKFDETKGFKF
                     ISYAVWWIRQSIMLGIVEHSRLIRLPLNKAGNVSKVNKLTSQFEQEFERDPTPEEIAE
                     TLHLKDDDVRDIIASNIRHISMDAPLGNDGDDGSMADILADTGEDTPDSGLVSESLRE
                     EIASALKVLSEREAEVVAAYYGISGYPSMTVEEIAKKFNLSRERVRQIKERAIRRLRK
                     ATMSSALKNYLG"
     gene            6560..7810
                     /locus_tag="PLL28_10620"
     CDS             6560..7810
                     /locus_tag="PLL28_10620"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNF69821.1"
                     /translation="MGADHYKFYTTIDAMEPMFPEDRSGELEYLATVLMMRASALSES
                     LHPLTRKAIADLLRPMNTYYSNVIEGHDTHPLDIEKALAANFLKNKRQAALQLEAKAH
                     INLHKLIAERFDLKSENPYKKEFICFLHKEFYRHLPGEFLHAERIDGTSVMVIPGQIR
                     DSEVSVGRHVGPYSKSLSAFLDRFEEYYDPTSNNNKSKIKRIISIAAAHHRLAWIHPF
                     VDGNGRVVRLFTDACFMSEDLHASGLWSMSRGLARHEAAYKFALSAADAGNMNDYDGR
                     GNLSNRELVNFCNFFLSTAIDQVEFMSRMLDIDHMIACIHRFVDLMVAHDKLKKEAAG
                     LLETVFLKGKISKSEAMRITGRSDKTTKLIADSLLEMGLLKLENETKFSDYLVNYPIG
                     FSTVIISGLYPRDKEIDILSRIIS"
     gene            complement(7859..9175)
                     /locus_tag="PLL28_10625"
     CDS             complement(7859..9175)
                     /locus_tag="PLL28_10625"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNF69822.1"
                     /translation="MKFFILGIVALWLLVTGGCRLQPDMGTDGPQIFFDEVPNLIDPA
                     HTKVLIVGVLIYDDPALGNFDTNHRKDEELASAFAAYGIPDSNIMMLQDGEATRNAMY
                     KALSKIAKNSDENTNFIFYFAGHGFNGWDDDASSIYFANSDIKYLHPDKTGFNLNFFT
                     EEFLPAFEGNSIWLMGDCCKSGGLNDVAANFGATGKQSVALCSCYYTDWSTGNWTFTQ
                     KIIDALNGDGMIDKEHDGTITLDDLIAGLSEGLQYVDRQRADCTMYNITGGAFVSDVT
                     IPYSEFTDPQVALGDYVFVYKKHSWYPIEITGKTGTTYTGRRYNFSDYETTTFTVETM
                     KIPYFVHYPIGAEILYDAYTDKPGVIIGADGDFMQMQSTEGGQILWRPYEVIITGTEI
                     PAAILNDDAVWTAGQVLDSLDGNYYVRYNDKNFQWDQWVTPDRVVF"
BASE COUNT         2684 a         1992 c         2072 g         2476 t
ORIGIN      
        1 attttatctc acaaggacca aattactgca gaggcggaaa aattacctat gctccaagtc
       61 taagattcta tctaaagacc tcatggtcct attttcggct gtgccaaccc actggctaaa
      121 tcgaaacaca acccaacctc gtttgcaaat tttccgaaag ctccttaact ttgcacccac
      181 aaatcgggga aacccgcgct ttttggctga tggtgtaact ggcaacacgt ctgattttgg
      241 ttcagaagag tctaggttcg agccctagtc ggccaactga aaggtctgca taaagcaggc
      301 cttttttgtt ttatcaccca cgtttataaa cccgaaaacc tgcaatattc tttttttagg
      361 cgaaacccgt aactttgccc ctcattcact tagaaatcat acattgacct atgtcgtata
      421 ataatattgt tagtctgctc ggcgataagg ccgaatacct cctcaaccac gaatctaaaa
      481 ccatcagtcg cgacagcctc cacctcccgg gtgccgattt tgttgatcgc gtatggatac
      541 cttcagaccg caatccacag gtgctgagga atatggaagc aatgtttggt cacggccgac
      601 ttgccaatac cggatactta agcattttgc ctgttgatca gggtattgaa catagcgcag
      661 gggcttcatt tgccaaaaat cccatttatt tcgatcccga aaatattgtg aaactggcta
      721 tcgaaggcgg ttgtaacgct gtggctacta cctatggtgc cctcggtgcc atcagccgta
      781 aatatgcaca tcgcatcccc tttatcgtaa aaatcaacca caacgaattc cttacttatc
      841 ccaataaatt tgatcaggtg atgttcacct ctattaaagc ttcttgggag cttggcgctg
      901 ctgctgtagg agcaaccata tacttcggtt ctcctgaatc tacacgccaa ttgcaggaag
      961 taagcgaggc tttccaatat gcgcatgaac tcggtatgtc taccatcttg tggtgctact
     1021 tgcgtaaccc tgcattcaaa caagccgata aagattatca cgtaagtgcc gatcttaccg
     1081 gtcaggctaa ccacctcggt gttactattg aggctgatat catcaaacaa aaattgcctg
     1141 aaaataatgg cggttacctc gccttaaaca gcaaagagtc tccttacgga aagaacgata
     1201 aacgtatcta cgaagaactc accagcgagc atcctatcga cctttgtcgc tatcaggtgg
     1261 caaactgctt tatgggtcgc gcaggtctga tcaactcagg tggtgcttct tcaggtgctg
     1321 ctgaccttac cgaagctgta accactgcgg taatcaacaa acgcgcaggc ggaatgggac
     1381 tcatcagcgg tcgcaaggct ttccaacgcc caatgaaaga aggcgttgag attcttaatg
     1441 cgattcagga tgtttacctg accaaggaag ttaccatcgc ataacgtata ttcgcaaacg
     1501 catcacccat gcaacccggg aaatccggaa tattttttca tgtaaacaag ggcgatcatg
     1561 gcagaacaga aacccgataa gtcgagctgg ttcaagaggg taaggaaagg gatagagact
     1621 tctacggaag agaaattaga tactccggaa ggggtgtggt ggcagtgccc ggaatgtaag
     1681 aaaacattta ccgccatcga atatgaaacc aacctctggt gctgtcctaa ctgcgcttac
     1741 cacggacgta tcggttcaaa acaatatttc agcatcctgt tcgatgaagg caaacacagc
     1801 ctcatttgcg aagaggtgcg agctaaagat gcactgaatt ttaaggacct gaaaccgtat
     1861 agcgacaggc ttaaagacgc ctggaaaaaa tctgagtata ccgatgcgat tactgttggc
     1921 acaggaccaa tcgacgggca tgatgctgtt atcgcttgca tgaattttga attcataggc
     1981 ggaagcatgg gatctgtggt aggggagcgc atttccaggg ctatcgacta ttgtgttgcc
     2041 cacaatcagc cactcctcat catatccaaa tcgggtgggg cgcgtatgat ggaaagtgct
     2101 tattcgctga tgcaaatggc aaaaacctcg gctaaactga ccttgctcga aaaacaccgt
     2161 ctcccttata tctccatttg caccgacccg actaccggtg gtgttaccgc cagctatgcc
     2221 atgcttggtg atgtaaatat cgcggaacct aaagccctga tcgggtttgc gggtcctcgg
     2281 gtggtgaagg aaacgattaa aaaagacctc ccgaaaggct ttcaatctgc cgagtttgtg
     2341 ctggaaaccg gattcctgga tcttattttg gaacgcaaat tgctgaaaag taatatcgcc
     2401 cgcctcctca agcacttccg caacgaagct agcgcctgat acagcgtgta cacccgataa
     2461 ttgtcagaag ttgctgatta tcaaatagtt accggaatgg gttcaccgaa gcaaggagca
     2521 gcggattaaa ctattaagtg cccgccggaa aaagtgtaaa agcctgaggt aggctgaagg
     2581 tgccttttct cccctcccaa aaaaggctgt tttcaaaccg gaatcaatgt ttagaagggt
     2641 taaaaaattt gtttgtatgg tagatagtgc gtatctttgt gcctcaaatt ttcaaaaagt
     2701 atagcgatgg ctgttttaac cgcagaaaag aaaaaagaga tatttaaaga ttttggtggc
     2761 aacgaaacca ataccggttc tgttgaagct caaatcgcat tgtttacaaa tcgtatcaat
     2821 cacctcactg atcacctgaa agcaaataaa aaagatttcg ctacaaagcg cagtctgctc
     2881 atgttggttg gtaagcgtcg tcgtttcctc aactacctga tgaaaaaaga catcaaggct
     2941 tatcgcgcgt tgatcgaaaa attgaatata cgtcgataat cgtcttatga aaatatgtat
     3001 gggctatgcg gatatttcat tattcccgta gcccattttt cttttgtagt aacccttaaa
     3061 caacaattta tttttaacct ggcaggagtt tgatcaaccc attttccccg ttgacaacaa
     3121 aaccccataa agatctttca aactcccgcc gcaaaatcac cctttaagga gggaattaat
     3181 ttaatcaatg aaaccaaatc caatcacgat tgaagtaccg ctgagcgaag gccgggtagt
     3241 gacgatcgaa acaggaaaac tcgccaaaca ggccgatggc tctgtagtgg tgcgctgcgg
     3301 tgatactatg ctcctggcta ccgttgtatc ggcaaaagaa gcgaaagatg gcgtagactt
     3361 tatgccgttg actgtcgact atcgcgaaaa ttatgctgct gccggtcgtt tcccgggagg
     3421 cttcctcaaa cgcgaggctc gtccttcaga gtatgaaatc ctcatcagcc gcttgataga
     3481 tcgcgctatg cgtccgatgt tcccggatga ttatcatgcc gatacgcaag tattggttac
     3541 actcatttca ctcgacaaag aaattttgcc tgatgcactc gcaggtttgt ctgccgcagc
     3601 tgcaatcgca gtttctgata ttccgttcaa tggcccgatt tctgaagtgc gcgttggacg
     3661 tatcgatgga aaattcgtta tcaaccctac acgtacagaa cttgaacgct ccgatatcga
     3721 tatgatcgtt gcgggaagta tcaaagacgt ggtgatggtg gaaggcgaga tgaaggaagt
     3781 gaaggaagaa gatatgctcg aagcaattcg cgttgcacat caggtgattc aggaacagtg
     3841 taaaaaattg aatgaactca ctacacttgt cggcaaagaa acaaaacgcg catacagcca
     3901 cgaagtaaat gatcaggatc tgaaagatcg tatgttccgt gaattgtatg ataaagtatc
     3961 tgcagttgca cacatgcctt cagcaaaaga agtgcgcggt gatgcattca aactcattaa
     4021 agaagaattc aaagctactt tcagcgaaga agaactcgca gaaaaaagtg gcatgataga
     4081 tcgctattat aaagacgttg aaaaacatgc catgcgcgaa atgatcctca ccgatcgtca
     4141 gcgcctcgat ggtcgccgta tggatgaagt gcgtccaatt tgggctgaag tggattatct
     4201 gccatcagca catggttccg cagtatttac acgcggtgaa acgcagtcat tgaccactgt
     4261 taccctcggt gcaaaaatgg acgagcagtt gattgatggt gttgtacatg aaggcacatc
     4321 aaaattcctt ttgcattata attttcctcc gttctctacc ggcgaagcaa aaccaatgcg
     4381 tggtttaggt cgtcgtgaag tgggacatgg aaatctcgca atgcgcgcta tcaaatggtc
     4441 gctgcctgaa gaaaatgctt ataccatccg tgtagtttcc gatattttgg aatctaacgg
     4501 ttcatcatca atggcaactg tttgcgccgg ttcattggcg ttgatggatg ccggtgtgca
     4561 ggtgaaaaaa cctgttgccg gtattgccat gggtcttgta tcagaagcaa acatggtgcg
     4621 ctacggaatc ctgagcgata tccttggcga tgaagatcac ctgggtgata tggactttaa
     4681 agtggctggt acggttgatg gtattactgc cacacaaatg gatatcaaag ttgacggatt
     4741 gagctttggt gttgtgaaag aagccctcga gcaggcgcgt gccggaaggt tgcacattct
     4801 caacgaaatg agtaaagcca ttcaaggtcc tcgccccgat cttaaaccgc atgcaccacg
     4861 tatcactacg attcaaattc cgcgcgatta tatcggagcg gttatcggac ccggtggtaa
     4921 gattattcag gaaatgcaac gcgagagcgg agcagaaatc agcatcgaag aaatcggcga
     4981 ttttggacat gtggttattt cttcacctaa tctcgagtcg atagaaaaag ccctcaaccg
     5041 catcagagcg attgtagcca tacctgaggt tggagaagta tacgaggcta ctgtgaaatc
     5101 gatcatgcct tacggtgcat ttgtagaatt cctgcccggt aaagaaggct tgttacacat
     5161 atctgaggtt gactggaagc gcttagaaag tcttgaaggc attttgaata aaggcgataa
     5221 actacaagtg aaattgctgg aagtcgatca gaaaaccggc aagttcaaac tgtcgcgcaa
     5281 ggtgctattg gaacgccctc cgcggccaca agctaataac gagcagcatt gatctgatca
     5341 agctgtttac cataaggcat ccggaaccac acggatgcct tttttcattt ctattggcgc
     5401 tgtgaacttt ttgtgcatca aatatcattt tatggtaaag agcaacgttt acccataact
     5461 tttgtaactt gtgaacgtaa acagtaatta aacaatccta agccatgcgt cagctgaaaa
     5521 ttactgccag cataaccagt cgcgaaaacg actcactgga aaaatatctt gccgagattg
     5581 gaaagataga tatgattacg cctgaagaag aagcagtgct ggcaaaacgc attcgcgaag
     5641 gagatactgt tgcacttgat acgctcacca aagcaaacct tcgatttgtt gtttccgttg
     5701 caaagcaata tcaaggtcac ggacttacac tcagcgatct gatcaacgaa ggaaatgttg
     5761 gtttaattaa agcggcaaaa aaattcgacg agacgaaagg atttaaattc atctcttatg
     5821 ccgtgtggtg gattcgtcaa tctatcatgt tgggtattgt tgaacattcg cgattaatac
     5881 gtttgccatt aaataaagca ggaaatgtgt cgaaggtgaa taaactcacc tcgcaatttg
     5941 aacaagaatt tgaacgcgat ccaacacctg aagaaattgc tgaaacattg catctaaaag
     6001 atgacgatgt gcgcgatatc attgcttcta atattcgaca tatttctatg gatgctccac
     6061 ttggcaatga tggcgatgat ggtagcatgg cagatattct tgccgacacc ggagaggata
     6121 cgcccgacag tggattggta agtgaatcgc tgcgcgaaga aattgcttct gcattaaaag
     6181 tgctttctga gcgtgaagct gaagtagttg ccgcatatta tggtatcagc ggttatcctt
     6241 ctatgaccgt tgaggagata gctaaaaaat tcaacctcag ccgcgaacgc gtgcgccaga
     6301 tcaaagaacg cgctatccgc cgcctgcgca aagcaaccat gagcagtgca ctgaagaatt
     6361 atttggggtg atttgctgtc agatcgaacc cggaatagaa tccggaaatt tattcatttt
     6421 aaatacggaa aaattttgca tttgagggca taaatatgcc tgaagtgcct tatttaattg
     6481 gattttatgt gattaatgga ctcggaattg aatacggaaa aaattactat ttgaatacgg
     6541 aaatttgtat ttttgcaggg tgggagcaga ccattataag ttttatacta caattgatgc
     6601 catggagcct atgtttccgg aagacagatc cggtgaactg gagtaccttg ccacagtcct
     6661 gatgatgagg gcctcagcac tgtctgaatc gctccaccca ttgacaagaa aggcgatagc
     6721 tgatttgtta aggcctatga acacctatta ttccaatgtc atagaaggtc atgatacgca
     6781 ccccctggat attgaaaagg cactagctgc caatttcctg aaaaacaagc ggcaagcagc
     6841 attgcaatta gaagcaaaag cgcatattaa cctgcataag ttgattgctg aaaggtttga
     6901 cctgaaatct gagaatccat ataaaaagga atttatctgc tttctacaca aggagtttta
     6961 ccgtcatttg cccggggagt ttttacacgc tgaaagaatt gatggcacat ctgtaatggt
     7021 aatacccggt caaataagag acagtgaagt tagtgttgga aggcatgtgg gtccttattc
     7081 gaaatcactt tctgcatttc ttgacagatt tgaggaatat tatgacccga catctaacaa
     7141 taacaaatcg aaaataaagc gcattatttc tattgccgct gcacatcaca gacttgcatg
     7201 gatacatccc tttgtggatg gcaatggtcg tgttgtccga ttgtttacgg atgcatgctt
     7261 tatgtctgaa gatttacatg cgtccggttt atggtctatg tcgcgcggat tggctcggca
     7321 tgaagcagca tataaatttg ctctttcagc agcagatgca ggcaatatga atgactacga
     7381 tggtcgcggt aatttaagca atcgtgagtt agttaatttc tgcaattttt tcctgtcaac
     7441 agcaatcgac caagttgaat tcatgtcgcg aatgcttgac attgatcata tgattgcatg
     7501 cattcatcgc ttcgtagatt taatggttgc acatgataaa ctgaaaaagg aagctgccgg
     7561 attattagaa acagtgtttc taaaaggtaa gatttcaaaa tcggaagcca tgcgcataac
     7621 gggcagatcc gataaaacaa caaaattaat agccgatagc ttattggaaa tgggattact
     7681 caaactcgaa aatgaaacta aattttccga ttatctggta aattatccta ttgggttttc
     7741 tacggtaatc atttcaggtc tctatccgag ggataaagaa atagatatct taagcaggat
     7801 tatttcttaa tcggattgcc ggcggtatac atatcaaaaa ccgcaaggca aattgatatc
     7861 aaaacaccac tctgtcagga gtcacccatt gatcccactg aaagttttta tcattgtagc
     7921 gaacataata attaccatca agtgaatcaa gcacctggcc tgctgtccac acggcatcat
     7981 cgtttaatat cgctgccggt atttctgttc cggtgatgat tacttcgtat ggtcgccaca
     8041 atatttgccc gccttcagta ctttgcattt gcatgaaatc gccgtcagcg ccaatgatta
     8101 cgcccggttt atcggtatag gcatcatata atatttcagc accgataggg tagtgcacaa
     8161 aataggggat tttcattgtt tccactgtga aggtcgttgt ttcgtaatcg gagaaattat
     8221 atcgtcggcc ggtatatgtt gtgccggttt ttccggtgat ttcaatcgga taccaactgt
     8281 gttttttata cacaaaaaca taatcgccca atgctacttg cggatcggtg aattctgaat
     8341 aaggaatagt tacatcgctg acaaaagcac ctccggtaat attatacatg gtgcagtcag
     8401 cccgctgtct gtcgacatac tgcaatcctt ctgacaatcc tgcaatcaaa tcatcaagcg
     8461 tgatggtgcc gtcgtgttct ttgtctatca tcccatcgcc gtttaatgca tcgattattt
     8521 tttgggtgaa ggtccagttg ccggtactcc aatcggtgta gtagcagctg cataaagcaa
     8581 cggattgttt tcctgttgcg ccgaaatttg cggctacatc atttaagcca ccgcttttgc
     8641 aacaatcgcc catgagccaa atgctattgc cttcaaatgc aggtagaaat tcttccgtaa
     8701 agaaatttaa attaaatcct gttttatcgg gatgtaaata tttgatatca ctgtttgcaa
     8761 aatagatgga agaggcatca tcgtcccaac cattaaatcc gtgcccggcg aaataaaaga
     8821 tgaagttggt attctcatcc gagtttttgg caattttcga aagtgcttta tacatggcgt
     8881 tgcgcgtagc ttctccatcc tgcaacatca tgatattgga atccggaatg ccataagcag
     8941 caaatgcact tgccagttct tcatcttttc tgtggttagt atcaaaattc ccgagcgccg
     9001 gatcatcata aataagcacg cccacaatca atacttttgt atgtgcaggg tctataaggt
     9061 ttggaacctc atcaaaaaaa atttgcggac catctgtccc catatccggc tgcaatcggc
     9121 aaccaccggt aactaaaagc cacaaagcca cgattccaag gatgaagaat ttcatggatt
     9181 tcatttagtg aagataggta attgcgttga ttcgaaaagg ttgt
//
LOCUS       DAOQHY010000129         9163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_3824_length_9163_cov_4.771959, whole genome shotgun sequence.
ACCESSION   DAOQHY010000129 DAOQHY010000000
VERSION     DAOQHY010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9163
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3824_length_9163_cov_4.771959"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /gene="ribD"
                     /locus_tag="PLL28_10630"
     CDS             complement(<1..1011)
                     /gene="ribD"
                     /locus_tag="PLL28_10630"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNF69823.1"
                     /translation="MHEHYMERCIELASRGLGFTAPNPCVGCVIVHDERVLGEGWHKQ
                     YGAAHAEVEAFASVHGSDVALLPESTVYVSLEPCNHHGKTPPCTDLLITHRVKRVVIG
                     SMDPNPIVAGAGIQRLRDAGIEVISGVLTDACRKLNAPFFTFHEQHRPFITLKWAESA
                     DGFLSGSQGATVHMSNHEADILVHKLRAEHMAILVGGATALNDDPQLTTRLWPGNNPL
                     RIIWDTRGDLPARLRVFDGTVPTWVFTTVKHAQYPNAAMYHFPEGKAPLLAIVAQMRA
                     SGIQSVLVEGGALTHKTFLQAGLWDQLVRIQTPYVAGEGTAVPDAEGLEKLSYRVQQV
                     GDN"
     gene            1219..2373
                     /locus_tag="PLL28_10635"
     CDS             1219..2373
                     /locus_tag="PLL28_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69824.1"
                     /translation="MKTSTLLLIAALIAVQSVSFAQTNTRSTAAVANPSVQGMALSPE
                     VAAKMLRIELLKTGLFQVWDEFDMDEALRNDAKYQTGCYGVMCLSELGNELNVNYVFS
                     GSIDKLADRIVINIKVVDVKNVSVQTSLTREFSREDEAIQRMFESMLCEMYQIPFDPE
                     VLATLQFNNGAVVKESYTKINNTGPRMGAAYMMGGLYEFANRPESQGGLDIAPVMSLI
                     GYQFEWQYVGTENFSALVECVVNFSGMEQGRFIPSVDILNGFRFGTAGWEFGFGPGFG
                     IITTSQGFFDEQNTFGKGADYYFSEKDWESYSFAQYGEVVDVSEINPNYSFSENFDAR
                     GDAKLNTFWIFAAGRTFRAGSLNIPVNAFYTLMDDGGYAGVSFGFNVVKK"
     gene            complement(2577..>5252)
                     /locus_tag="PLL28_10640"
     CDS             complement(2577..>5252)
                     /locus_tag="PLL28_10640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69825.1"
                     /translation="FTLSEPGDVTLSINKLPGQYNTLYLLNSVGATLESQSNYGAFSI
                     TENDLPAGVYYARLHYYSTSHFGGYALTNSYCPDVITIEATGETTFCDGESVVLSTPD
                     HHLSYLWNDGSVTETNTVVISGDHYLTVDNGGGCVRVSNTITTESTPLPVAIIETDGP
                     VEFCDGGDVTLSVPALADTYLWSNGATTPTITVNETGDYSVILTKNGCTAISDPIHIT
                     VNALPVASVSADGMLTFCDGESVTLTANEAAAYLWSNGETTASILVTGSGDYSVEITD
                     DNGCMDMSEVTTVTVNANPIAAINADGPTTFCSGESVTLTASGGDTYLWNNGETTSSI
                     LVTTSGLYSATVYTAAGCSDITSTIEVVVEACGSVTIVAEGPTEFCDGNTVTLTSSEI
                     TGNVWNTGETTQSIVVDASGDYYCTNGANTSNTITVTVFANPIATITPDGATAFCAGG
                     SVMLNASAAESYLWNTGATTASILADASGDYSVTVTDINGCFATSMVTTVIEYTEPVA
                     TISAGGPTTFCEGGNVELTASEAESYLWNTGATTESLTASSTGAYFVTITDINGCTAT
                     SGTITVTVNPNPTPVISADGPVLICGTDVTLSADAVYDTYLWSNGETTPEITTGTSGV
                     YSLTVTSGGCSGVSNTIEVVADVAPTVTIESESTLICFEASTMLTATTDGANIQWQRN
                     GVDIPGATDAVYYASENGKYRAVVTTGACESTSSETKLKYAERLTISPAGVTDLCGGP
                     VTMNIPAMPGLTYQWYRNNTLIAGATGNAYTTTIPGKFYCLANADGCTRASKLLVVND
                     DCRNGNIVALEMSLWPNPASDYFTVSLQGMSAGDAMLRVSDIAGQVIFTESVYINRID
                     QTFTIPSTDLPSGMYCVTFQDAPGNLTTQRVIIQR"
     assembly_gap    5253..5352
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5353..6764)
                     /locus_tag="PLL28_10645"
     CDS             complement(<5353..6764)
                     /locus_tag="PLL28_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69826.1"
                     /translation="MKLRNLLFAVALLMTCISPVWADTEPDNDLAAGADAMTVGDPAT
                     GTINMSGDLIDFYAFTTSDDGTISLDLTSDVTYLTIRLYDADGVTVLQTASNYSNVSL
                     SRTGLAAGNYYAAVVAYAGYSGTYDITATVTAAPYMNDTESNNTYLLATDLTENGSTE
                     GHIGYRYNGGTYDTDDWYKVVTTNDGAITATFANTFGAYNTIYIYDNDGTTVLGSSSN
                     YGSSSITIPGKAAGTYYIRLYYYSTSHFNGYTLSNTVTPTAYDNDAEPNDLFSEATVT
                     IPENDSVSGHIGHRYNGGTYDTDDWYLLELTQDGQINLNLNYGFGQYHGIYLYDSDGT
                     TVLANNFNYNAATVTRNNLSAGTYYAKVDNYSSSYHSGYILEFEAIPAAYANDTEPND
                     VYTQANTDIPEGGTVYGHIGFYENGGTYDTDDWFTFTTTQDGIVSLTLNADPGQYHGI
                     YIYDNNGTTLLSNDFNYTSVT"
     gene            complement(6872..7510)
                     /locus_tag="PLL28_10650"
     CDS             complement(6872..7510)
                     /locus_tag="PLL28_10650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF69827.1"
                     /translation="MDIRVVIFDDNRTRRDGVSLLLNTSDGYACVGAFEDFAKGLEQI
                     EQLMPDVIIMDIDMPGINGIEAVTRIKSRFPEQKVMMQTVFDTDDKVFESILAGASGY
                     LLKKASPVKLLESIREIHEGGSPMTPEIATKVLQFFRTRERQRGAGQQLLTAKEQEVL
                     SDLVNGLSYKMIADKQSSSYHTVNFHIRNIYKKLHVHSIGEAVAKAIRDRLV"
     gene            complement(7575..>9163)
                     /locus_tag="PLL28_10655"
     CDS             complement(7575..>9163)
                     /locus_tag="PLL28_10655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HNF69828.1"
                     /translation="EHVYYDIIQYTNVPGTLSNNFVRDIYRDRSGNIWVATSEGLNKW
                     NKSMPVKNAFTIFKYHTQDPHSISHNNVSAIWQAPDGMLWLTTSNGLNAFDGKRFTRY
                     YGNSNQFRQMYGIYPDKKGNIWIPTRDGFEVFDLSKKTFRHVSMINPAWALKSPTRML
                     QTSSGTWMYGVGNYLISFTPDTYVFDTTFPKLYIKELSVDDKHLFEELNMDALALRHN
                     EDVISIVFSCLQLTHPGTAKYMYKLVGQADSWIELGNNNAINFLDLSPGTYTLYIKVT
                     NPQGDWSGPVKMLQFTVLPAFWQTWWFYLLCTLSILGIGWAVVRYRELQLKRVLLMRN
                     KIANDLHDDVGSALSTINIFGEVAAKKSADNPELRSIIDKITDISTEMQENMTNIVWS
                     LQPRNDNFDQMMLRIKSYALEHLAIQQIDVHFSIDERLSGLKIAANKRKEMFLIYKEA
                     LNNILKYASATTVHISFKRNGADVVMEITDNGKGFDPEELHAGNGMFTMRERAALIDG
                     QLHIRSASGKGTSVILQCKC"
BASE COUNT         2410 a         2122 c         2077 g         2454 t
ORIGIN      
        1 attatctccc acttgctgca cacgatagct cagcttttcc aacccctcag catcaggcac
       61 cgccgtcccc tcacccgcta cataaggcgt ttgtatccgc acaagttgat cccataaacc
      121 tgcctgtaga aaagttttat gggtaagggc accgccctcc acaagtacac tctgtatccc
      181 acttgcacgc atttgtgcta ctatggcaag cagcggagcc ttcccctcgg gaaagtggta
      241 catcgctgcg ttcgggtact gcgcgtgttt aacggtggta aatacccacg ttggcaccgt
      301 accgtcgaaa acccgcaatc gcgcgggcag gtcgccacga gtatcccaga tgatgcgcaa
      361 cggattattg ccgggccata atctagtggt caattgcgga tcatcgttca gggcagtggc
      421 acctccaacc aaaatggcca tgtgctctgc ccgcagttta tgcaccagga tatccgcttc
      481 gtgattcgac atgtgcacgg tagcgccctg gctgccactc aggaagccat cggcactctc
      541 tgcccatttc agcgtaatga agggcctgtg ttgctcgtga aaggtaaaga aaggggcatt
      601 gagctttctg caggcatctg tcaatacacc ggagatcacc tctatgccgg catcacgcaa
      661 ccgctgtatc cctgcacctg ccacgatcgg attcggatcc atggacccga tcaccacgcg
      721 ctttactcta tgcgtgatga gtagatcagt gcagggtggt gttttaccat ggtgattaca
      781 gggctcaagg ctcacataca ccgtggattc aggcaacaga gccacatctg aaccatgcac
      841 ggaggcaaag gcctccactt ccgcatgtgc agcaccatat tgcttgtgcc agccctcccc
      901 caacactcgc tcgtcatgca cgatcacgca gcctacacaa gggtttggag cagtaaagcc
      961 caagcctctg gaagccagct cgatacatcg ttccatgtag tgttcgtgca tgctgcaaag
     1021 gtagagaatc aagccggcaa cacatgcccg ttacgacttt ctgccaaaat cccgcgagcg
     1081 gaaaggcaaa agttgcggtt tttagactga cggtccggtg ttttagtaaa aatcgcaaat
     1141 accggtacga tcaagttgcg ctacaccatt tgagtcgcct tatctatccg gatacatttg
     1201 ccccataaac ttaaatcaat gaaaacttca acacttttac tgatcgccgc acttatagct
     1261 gtgcaatcag tttccttcgc ccagacaaat acacgttcca ctgctgctgt tgctaatcct
     1321 tctgtgcaag gcatggcact ctcccctgaa gtagccgcga agatgttgcg cattgaatta
     1381 ttaaaaacag gcttatttca ggtttgggat gaattcgaca tggatgaagc cctgcgcaac
     1441 gatgccaaat accaaaccgg ttgttatggc gtgatgtgct taagtgagct cggcaacgaa
     1501 ctcaatgtca attacgtatt tagtggcagc atcgacaaac tggccgaccg cattgtgatc
     1561 aatatcaaag tagtggatgt aaaaaatgta agtgtgcaaa cttcacttac acgcgaattc
     1621 tccagagaag atgaagccat ccagcgcatg ttcgaatcta tgttatgcga aatgtatcaa
     1681 ataccattcg atcctgaagt gctggccacc ttacaattca acaatggcgc agtcgtaaag
     1741 gaaagttaca ccaagatcaa caacaccgga ccacgtatgg gtgctgctta tatgatgggc
     1801 ggattgtacg aatttgccaa tcgtcctgaa agtcagggtg gtttagatat cgcaccagtg
     1861 atgagtctga tcggttatca gttcgaatgg caatatgtgg gcacagaaaa tttctctgca
     1921 ctggtagaat gcgttgtaaa cttttcaggt atggaacaag gacgttttat tccttcagta
     1981 gatatattga atggtttccg tttcggtacc gcaggttggg aattcggatt tggtcccggt
     2041 tttggtatca taaccacttc acagggtttc tttgacgaac aaaatacttt tggcaaagga
     2101 gccgactatt acttcagcga aaaagattgg gaaagttaca gtttcgcgca atacggagaa
     2161 gtggtggatg tttcggaaat taatccaaat tattccttct ccgaaaattt cgatgcccgc
     2221 ggcgatgcca aactcaatac cttctggata ttcgcagcag gccgcacctt ccgcgcaggt
     2281 tcactcaata tacctgtaaa tgcattctat acattaatgg atgacggagg ttatgcaggc
     2341 gtgagctttg ggtttaatgt ggtgaagaaa taaaagtgaa agcccccatc tacaaaggac
     2401 tgtttgttga tcaaacagtc ctttttttta caccgccacc cgtgtaacca ccacatcaga
     2461 aacatcatac atgttcacta tgcaacatct gctatctgca taaggcatca ccataccgaa
     2521 aaaagggctg ccttgatata caaagcagcc cattttttac atctttgaaa ttaaaatcaa
     2581 cgttggatga tcacgcgctg agtggtcaga ttacccggtg cgtcctggaa tgttacgcag
     2641 tacattcctg aaggcagatc agttgatggt atggtgaatg tttgatctat ccggttaata
     2701 tataccgact cagtgaaaat cacctgtccg gcaatatcac tcacacgcaa catggcatca
     2761 ccggcactca taccttgcaa gcttacggta aagtagtcgc ttgccggatt cggccaaagg
     2821 gacatttcca atgctacaat atttccattg cggcaatcgt cgttcaccac caataatttt
     2881 gaggccctgg tgcaaccgtc agcatttgcc agacaataga atttacccgg aatggtagtg
     2941 gtatatgcat tgccggtagc ccccgcaatc agggtattgt ttctgtacca ttgataggta
     3001 agtccgggca tggccggaat gttcatggtt accggtccac cacagagatc agttacaccc
     3061 gcaggtgaaa tggtcagtcg ttctgcatat tttaatttgg tctcagagct ggtactttcg
     3121 catgcaccgg tagttaccac tgcgcgatat tttccatttt cggatgcata atacaccgca
     3181 tccgtagcac caggaatatc aacgccattt cgttgccatt ggatatttgc cccatcagtg
     3241 gtagctgtaa gcatagtgct tgcttcgaaa caaattaaag tgctttccga ctcaatggtt
     3301 acggttggtg caacatccgc aaccacttct atagtattgg aaacaccgga acaaccacca
     3361 gaggtaactg tcaaactata aacaccgctg gtaccggttg taatttctgg tgtagtttcg
     3421 ccattgctcc acagataagt atcgtagact gcatctgcgc tcagtgtaac atctgttccg
     3481 caaatgagca ctggtccgtc ggcacttatc accggtgttg gatttgggtt aaccgttact
     3541 gtgatcgtac cggaagttgc cgtgcagcca ttgatatcgg tgatggttac aaaatacgct
     3601 cctgttgaag atgccgtgag cgactcagta gtagctccgg tattccagag atatgactca
     3661 gcttccgatg ctgtcaattc aacattaccc ccttcacaga aagtagttgg accaccggcg
     3721 ctgatggtgg caaccggttc agtatattca atcaccgtag ttaccatgga agtagcaaaa
     3781 catccgttga tatcggtaac agttacggaa taatcaccac tggcatctgc caaaatcgat
     3841 gcagtagtag cgcctgtatt ccagagatac gactcagcag cagacgcatt cagcatcacg
     3901 cttccacctg cacaaaaagc agttgctccg tcgggtgtga tcgttgcgat cggattcgca
     3961 aacacggtta ccgtgatcgt attcgatgta tttgcgccat tcgtgcaata ataatctcct
     4021 gaagcgtcca ctacaatgct ttgagtggtt tcaccggtat tccaaacgtt gccggtaatt
     4081 tcggatgaag taagggtaac tgtattgcca tcgcagaatt ctgttggccc ttcagcaacg
     4141 atggtaacgc taccgcatgc ttcaacaact acttcgatag tggaggtaat atcagaacat
     4201 ccggctgcag tataaacagt cgcagaatac aaacctgagg tagttacaag aatagacgaa
     4261 gtggtttctc cgttgttcca caaatacgta tcaccaccgg atgcagttaa agtaacggat
     4321 tcgccgctgc agaaagtggt tggtccatca gcgttgatcg cagcaattgg atttgcattg
     4381 acagttactg ttgtaacttc tgacatatcc atacatccat tatcgtcggt gatctccacg
     4441 gaataatcgc ctgagccggt aaccagaatg gaggcagttg tttcgccatt gctccataaa
     4501 taagcagcag cttcgttagc agtaagtgta acgctttcgc catcacagaa agtaagcatg
     4561 ccatcagcag aaacgctggc aacaggaagt gcatttactg taatgtgtat cggatcggag
     4621 atagcagtgc aaccgttttt ggtgagtatg acactataat caccggtttc atttacggtt
     4681 atggttggtg ttgttgcgcc attgctccag agataagtat ctgcaagtgc cggaacgctt
     4741 agggtaacat cgccgccgtc gcagaactca acgggtccat ctgtttcgat gatggcaacc
     4801 ggaagcggtg ttgattcggt tgtaatggta tttgaaacac gcacacaacc tccgccatta
     4861 tcgacggtga ggtaatgatc gcctgaaatg actacggtat tggtttcggt gacagagcca
     4921 tcattccaca aataagacaa atgatgatcc ggtgtgctga gcactaccga ttcgccatcg
     4981 caaaatgtag tttctccggt agcttcaatg gtgatcacat cagggcaata actattggtg
     5041 agtgcgtatc caccgaagtg actagtggaa taataatgga gtcttgcata atacacgcct
     5101 gcaggtaggt cattctccgt gatggaaaag gcgccgtaat ttgattgaga ttccaaagtt
     5161 gcgcccacac tatttaataa atacaaggta ttgtactgtc cggggagttt attgatagaa
     5221 agcgttacat cgccgggttc actcagtgtg aannnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nngtaacgga ggtataatta aaatcattgg acagcaaggt ggtgccatta
     5401 ttatcataga tataaatacc atgatattgt ccagggtctg cattcaaggt cagacttacg
     5461 ataccatcct gagtggtggt gaaggtgaac cagtcatctg tatcgtatgt gcccccattt
     5521 tcataaaatc cgatatgacc ataaacggtg ccgccttcag gaatgtcggt atttgcctgg
     5581 gtgtatacat cattcggttc cgtatcattc gcatatgcgg caggaatggc ttcaaactca
     5641 agaatgtaac cggaatgata gctggaagaa taattgtcaa cctttgcata ataagttccg
     5701 gcggataaat tattccgggt aactgtggcc gcattataat taaagttgtt tgcaagcacg
     5761 gttgtaccat cgctatcata taaataaata ccatgatatt gtccgaaacc ataattcaga
     5821 ttcagattga tctgtccgtc ttgtgttaat tcgagcagat accaatcatc agtatcatag
     5881 gtgccgccat tgtatcgatg gccgatatgg ccactcacac tgtcattttc aggaatggtg
     5941 accgtagctt cagaaaaaag atcattaggc tcagcatcat tgtcgtacgc ggttggtgta
     6001 accgtattgc ttagggtata gccattaaaa tgtgacgtgg aataataata cagtcggata
     6061 taataagtac cggctgcttt tccaggaatg gtaatgctgc tactgccata attagagctg
     6121 ctgcccaata cggtagtacc atcattgtcg taaatataaa tggtgttata cgccccaaag
     6181 gtatttgcga atgtggcggt gatggcaccg tcattggtgg ttacgacctt gtaccaatca
     6241 tccgtatcat aagtgccccc attataacga tagccgatat gtccttcagt acttccattt
     6301 tctgtcaggt ctgtagcgag cagataagtg ttattacttt ctgtatcgtt catatagggc
     6361 gctgcagtta ctgtagctgt aatgtcgtaa gtgccactat atccggcata ggctacaaca
     6421 gcagcataat aattaccggc agcaagtccg gttctggata aggaaacgtt gctataatta
     6481 gaagccgttt gcaacaccgt tacgccatcc gcatcataca ggcgaatggt gaggtatgtt
     6541 acatcgctgg tcagatcaag tgaaatggtt ccatcatcac ttgtagtgaa tgcatagaag
     6601 tcgatcaggt cgcctgacat gttgatggtt cccgtggccg ggtcgccaac cgtcattgcg
     6661 tctgcccctg cggcaagatc gttatcaggt tccgtatctg cccataccgg gctgatgcag
     6721 gtcatgagta atgcaacagc aaaaagtaga tttcgaagtt tcataagaaa agattagggt
     6781 tcaataattc cggggtattc aatcctcaaa attatggcta agctgtatcc tgtgtaaccg
     6841 caaaatctag caggtaagcg cagtggtctg attataccaa gcggtctctg atcgccttgg
     6901 ctactgcttc accaatgctg tgtacgtgga gctttttgta gatattccga atatggaaat
     6961 ttacggtgtg ataactggag ctttgcttat cggcgatcat tttataactc agtccgttca
     7021 ccagatcact cagcacttcc tgttcttttg cagtgagtaa ttgttgtccg gcgccgcgtt
     7081 ggcgttcgcg tgtcctgaaa aattgtaaca ccttggtagc gatttccggg gtcatgggtg
     7141 aacctccttc gtggatctcg cggatagatt ctaacagttt tacaggtgaa gctttcttga
     7201 ggagataacc ggatgcgccg gccaggatgc tttcaaatac tttgtcgtcg gtatcaaaaa
     7261 ctgtttgcat catcactttc tgttcaggga atcgcgactt gatgcgggta acggcctcta
     7321 tgccgttgat accgggcatg tctatgtcca taatgatcac gtcaggcatc agttgttcga
     7381 tctgttcgag ccctttggca aaatcctcaa acgcccctac acaggcatag ccatcggagg
     7441 tattgagcaa aagcgacacg ccatctcttc tggtgcgatt gtcatcaaaa atgaccactc
     7501 ggatatccat agaaattcat tacaccaaaa ataaagcaaa acagtccaat cctacccgca
     7561 aaatttagta ggtatcagca tttacattgc aatatcacac tggtgccctt acccgatgct
     7621 gagcgtatgt ggagttgccc atcgatcaat gctgcacgct cgcgcatggt gaacatgcca
     7681 tttcctgcat gcagttcttc gggatcaaat cctttaccgt tgtcggtgat ctccatgact
     7741 acgtcggcac cgttgcgttt aaaggagata tgcacggtgg tagcagaggc atatttgagg
     7801 atattgttca gggcttcctt gtagatcaaa aacatctcct tccgtttatt ggcagcgatc
     7861 tttaaaccac tcagtcgctc atctatactg aaatgcacat ctatctgctg aatagctaaa
     7921 tgttcaagcg catacgattt gatacgcagc atcatctgat caaaattatc attgcgtggt
     7981 tgcagactcc agactatgtt ggtcatgttc tcctgcatct ctgtgctgat gtcggtgatt
     8041 ttgtcaatga tggatcttaa ctcaggatta tcggcagact tctttgctgc tacttctcca
     8101 aatatgttaa tggtgctgag tgcgctgcct acatcatcgt gcagatcatt ggcgatcttg
     8161 tttctcatca gcagcacacg ttttaattgc aattcgcgat acctgaccac cgcccatcct
     8221 ataccgagga tgctaagtgt gcatagcaga tagaaccacc aggtttgcca aaacgcaggc
     8281 aacacggtaa actgcagcat ttttacagga ccgctccaat cgccttgcgg gttggtcact
     8341 ttaatatata aagtgtaagt acccggcgac aggtccagga agttgatggc gttgttattt
     8401 ccaagctcta tccagctatc cgcctgccca acaagcttgt acatatattt tgccgtgccg
     8461 ggatgcgtta attgcaaaca ggaaaataca atggaaatca catcttcatt gtgacgcaac
     8521 gccaaagcat ccatgttaag ttcttcgaac aggtgtttat catcaaccga taactcttta
     8581 atatataatt tcggaaaggt cgtatcgaat acataagtat ccggtgtaaa ggagatgagg
     8641 taattgccca cgccgtacat ccaggtgcct gaggaagttt gcagcatacg ggtgggtgac
     8701 ttcaaagccc aggcaggatt gatcatagac acatgcctga aagttttttt cgacaggtcg
     8761 aatacttcaa aaccatctct ggtagggatc cagatatttc cctttttatc cggatatatc
     8821 ccatacatct gcctgaactg attggaattt ccgtaatagc gtgtgaatcg cttaccatca
     8881 aacgcattca atccattgga tgtggtcaac cagagcatgc catcaggtgc ctgccagata
     8941 gcagatacat tattgtgcga tatggaatgc ggatcctgcg tatgatactt gaatatggta
     9001 aatgcattct tcaccggcat ggatttattc cacttattca gtccctcaga agtagccacc
     9061 cagatatttc cggaacgatc acggtatatg tcgcgaacaa aattattgga aagtgttccc
     9121 ggcacgttgg tatattgaat gatgtcataa tacacatgct ccg
//
LOCUS       DAOQHY010000130         9089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_3870_length_9089_cov_6.809608, whole genome shotgun sequence.
ACCESSION   DAOQHY010000130 DAOQHY010000000
VERSION     DAOQHY010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 9089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9089
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3870_length_9089_cov_6.809608"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /gene="nadD"
                     /locus_tag="PLL28_10660"
     CDS             <1..587
                     /gene="nadD"
                     /locus_tag="PLL28_10660"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HNF69829.1"
                     /translation="YLCYMRIGLFFGSFNPVHTGHMIIANYFAENTDLQQVWFVVSPQ
                     NPFKDKQSLLDEKHRLYMVNLAVEQHYKLQASNIEFHLPQPSYTINTLTYLKEKHPDY
                     TFVLLMGSDNLSSLHKWKNIDLILASHDIYVYARRGVETIPDGLGGNIRLFQLPMLDL
                     SSTTIRQFIKEGKSIRYLVPDVVWNHINEMRFYR"
     gene            698..1123
                     /locus_tag="PLL28_10665"
     CDS             698..1123
                     /locus_tag="PLL28_10665"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HNF69830.1"
                     /translation="MRLTLAMICAFMVSMSAKAQNWQDNILGGWINGDKDAKVEIYVK
                     DGKYYGKITWLRNPNEEDGTPKVDDENPDESKQNEPIMGLVILKGFDFVDGMWKNGTI
                     YDPKNGKTYKCEMWLDGKELKIRGYWGIVYRTETWTKPK"
     gene            1145..1510
                     /locus_tag="PLL28_10670"
     CDS             1145..1510
                     /locus_tag="PLL28_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69831.1"
                     /translation="MKTQTLLRAGTLVFFFCMIGGFVAYKSGLIQLSENNIPQAQQPD
                     YINTLASNTKGVIDTPPGLQTTLPEIPDSVFRIMMSSKTMIILDQSSLFPDNDNQFPE
                     HFYDDSIFELPRITLDSLK"
     gene            1531..2301
                     /locus_tag="PLL28_10675"
     CDS             1531..2301
                     /locus_tag="PLL28_10675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNF69832.1"
                     /translation="MEYLRIIGGTGLSLIFLAIVFIPMEKVFPAKPGQKILRPYWKTD
                     LCYFLGQYLLWGFLVLWAMMQLSFFLDDIVPHSFRTAVQLQPKWLQIIEVVLGSDLLI
                     YWGHRLQHRIDFLWRFHKIHHSAEHLDWLAAHREHPLDSLYTIGLINLPAFLLGFHLD
                     LLAGLIAFRGIWAIYIHSNVRLRLGPLRVLIGSPELHHWHHDLDRDRGNYANISPLMD
                     VIFGTHVFPDHEPEHFGIKEEFPKSYPAQLLAPFKRTK"
     gene            2429..5725
                     /locus_tag="PLL28_10680"
     CDS             2429..5725
                     /locus_tag="PLL28_10680"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNF69833.1"
                     /translation="MFTSTKKKLLTLSACVSVFAYQHADAQIDAGLFRFPDVSATQIV
                     FTYANDVWIAPIEGGTAIKLSSPSGVESFPKFSPDGKTIAYTANYDGNWDVFSIPTTG
                     GLPARLTQHGWTDRVVDWTPDGKSVLFASGRESEKERYSKLFTIPAQGGAPTTLPLAY
                     GEFASYNADGSSMALVFRSEAFRTWKRYRGGDVADIYIFNFADNSSYNISGDIDAGEE
                     FPMWYGNAIYFLSDNGSEKRMNIWKYDMATKSRTQITHFTDYDVHFPSMGPNDIILEA
                     GGKMYLYGLQSGNLREVKLNVVTDNTNLKPHTENVAALMQNMQIGPDGNRVVVEARGE
                     LFSIPAENGYVKNLTRNSGTAERYPAWSPNGKWIAYWSDASGEYELWTMDMENNGATK
                     QITHLGKGYRYYIYWSPDSKKVVFVDQAMKIWMVDMATGVATNVDHGLQQAHYNLNGF
                     TANWSPDSRYIAYLRDLDNAYYAAFIYDTQDKTIHQVTSGFYNCSEVTFDPSGKYLYV
                     ATGQSFSPTYSDLDNSFIYPNTTELAAISLKKDTPSLLVAKNDMVEIKKEEAKEEKAD
                     DKKDKNKKDKSEKEEDSASTIAPTEIDWDGIEGRMVLLPIPAGNMYNVSGVEGKIIFI
                     RYPNTGTAEGSTPSLKYYDIEKREEKTILDGVWDYALTADGKKVLVMGDGMQAIVAAE
                     EGQKFEKPLRINEMEMVVDPMAEWKQIFMDTWRIERDYFYDVNMHGVNWEQVKERYLK
                     MLAGATTREEVNVVLGDMIAELNSSHAYRFGGDLEATPMANCGYLGVNWTADGQYYRV
                     GKIIRGASWDAEMRSPLDAPGIGIVEGDYILAVNGNPILTTQEPFAYFQGLGGKTVEL
                     TYNNKPSMDGAKTVVVTTLDYEGRLRNLAWIEQNRKRVEEASNGQVGYIYVPSTGVDG
                     QNELIRQFRAQWDKPALIIDERFNNGGQIPDRFIELLDRDPLAYWAIRDGKSWQWPPL
                     AHFGPKVMMINGWSGSGGDAFPDFFRQKNLGPLVGQRTWGGLIGISGAPTLIDGGEIT
                     APTFRMYYQNGTWFAEGHGVDPDIKVEEDLTSLAKGKDAQLERAIEEAMKLIKTEGYQ
                     APARPAPEVR"
     gene            5818..7071
                     /locus_tag="PLL28_10685"
     CDS             5818..7071
                     /locus_tag="PLL28_10685"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNF69834.1"
                     /translation="MRKQIPLFIFVLLAGSMSAQITKSAFFVGNSYTATNNLPQLISD
                     VAESAGDVLLYNSNAPGGYTFEMHTTNTTTLEQIALGTWDYVVLQEQSQLPSFPIDQV
                     EVDCFPFATTLDSLIRVANTCTETMFFMTWGRKDGDASNCAFWPPVCTYNGMDSLLHE
                     RYMTMGEDNDAEVSPVGAVWHYLRDTHPEIELYIGDGSHPSAAGSYAAACTFYAAIFR
                     NDPTLITYDFTLPADQAHIIREATKLVVYDSLASWYIGTYIHEPIANFNVNTGIPFTA
                     SFENLSENAISYLWNFGDGTNSTEAAPVHIYGAEGMYIVSLEVTGCSDVDDYTSDITI
                     TGVETSIQNNAIINWQPENPVHAELFLPANNSNWRIEILDMAGNVVMQTYAQTLITVD
                     GIHAGAYIIYLQQGDERMYRKLMIQ"
     gene            complement(7074..7952)
                     /locus_tag="PLL28_10690"
     CDS             complement(7074..7952)
                     /locus_tag="PLL28_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69835.1"
                     /translation="MVQRLLIACLVLAGIIGCKHRKDPGPLTDGTETLIENDVYVVHS
                     MIYADEKPWTTSNIFVFKGLGDTIWIYGSGYGDFVDPCGDCNDNNFYLGDNMHGTGPA
                     TVDVEQVDSVITKIFGRNRDSVLLQFIVPHYHNDHINNEFVDAFHSSFRYPLRSDEKI
                     WIHTNDYYGATCNEPCCGTQPCPDKKHNKYFGVPYLPAWKPEYLALFTKMGSENDDCD
                     TALKSFTTASGSWTITKAMSTADGGHTDGTVNIKSFDKKILIAGTKSKIQCPYPDDWQ
                     FVSVHGNIVGATTGNE"
     gene            complement(7952..>9089)
                     /gene="serS"
                     /locus_tag="PLL28_10695"
     CDS             complement(7952..>9089)
                     /gene="serS"
                     /locus_tag="PLL28_10695"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNF69836.1"
                     /translation="DNTLAETNSTSKEIGKLMGQGLRDEAEAKKQHVADLKQRSAALE
                     TGHADIQEQIKNILLSLPNLPQSLVPQGKDAEDNLEVRGGGNKPQFSFEALPHWELAA
                     KYRLIDFELGNKITGSGFPVYLGKGARLQRALTQYFLDQAAAAGYMEVVPPFMVNADS
                     GYGTGQLPDKEGQMYHATEDDFYLIPTAEVPVTNIYRDTIVKEEDLPIKMTAYSPCFR
                     REAGSYGKDVRGLNRLHQFEKVEIVEIAHPNTSAERLDVMVAHVEKLVQSLELPYRIL
                     RLCGGDMGFASAITYDFEVWSGAQQRWLEVSSVSNFESFQANRMKCRFKDSSDGKNKL
                     VHTLNGSALALPRILASILENNQTEGGIDIPKVLVPYTGFDKIS"
BASE COUNT         2668 a         1770 c         2088 g         2563 t
ORIGIN      
        1 tgtatctttg ttacatgcgg ataggcttat tttttgggtc ttttaatcct gtgcacacag
       61 gtcacatgat cattgccaat tattttgccg aaaacaccga cctgcagcag gtatggtttg
      121 tggtgagtcc gcaaaacccc ttcaaagaca agcaatcgct gctggatgaa aaacacaggt
      181 tatatatggt gaacctggcg gtggagcagc attacaagct gcaggcctcc aacattgagt
      241 ttcacctgcc acagcccagt tataccatca acacgcttac ctacctgaaa gaaaagcatc
      301 ccgattacac ctttgtgctg ctgatgggca gcgacaatct gtcaagcctg cacaaatgga
      361 agaatataga cctcatactg gcttcgcacg atatttatgt atatgccagg aggggggttg
      421 aaaccattcc ggatgggttg ggaggaaata tcaggttatt tcagcttcca atgctggacc
      481 tcagctctac cacaatacga cagtttataa aagaaggaaa atcaatacgt tatctcgttc
      541 ccgatgtggt gtggaatcat attaatgaga tgcggtttta tcgatgatgg ctggtaagcc
      601 ttatcgcagt ataagaataa tatgtcagca ggcgttttat ataaccggaa agaaatttcc
      661 gggagaatcg tatttttagg gaacaaacaa gaatgatatg cgactgactt tggcaatgat
      721 ttgtgcgttt atggtaagca tgagcgctaa ggcacaaaac tggcaagata atatattagg
      781 tggctggata aatggcgata aggatgccaa ggttgagatt tatgtgaaag acggaaaata
      841 ctacggcaag atcacctggc tacgcaatcc gaatgaagaa gatggcacac caaaggttga
      901 tgatgaaaat ccggatgaat cgaagcagaa cgaacccatc atgggattgg taattttaaa
      961 aggtttcgat tttgtagatg gtatgtggaa gaacggaacg atctatgatc ccaaaaacgg
     1021 aaaaacatat aaatgtgaaa tgtggctaga tggtaaagaa ttaaagatcc gtggatattg
     1081 gggcattgta tatcgcacag aaacatggac caagcctaaa taaagataaa ataaacgcgc
     1141 taacatgaaa acacaaacat tgctgcgcgc aggcacactc gttttctttt tctgtatgat
     1201 aggagggttt gttgcgtata aatccggatt aattcaactt tcagaaaata atattcctca
     1261 ggcacaacaa cctgattata tcaatacact agcaagcaat accaaaggcg taattgatac
     1321 accacccggg ctgcaaacca cactaccgga gattcctgac agcgttttta gaattatgat
     1381 gtcgtcaaaa acaatgatca ttcttgatca gtcatcattg tttcctgata acgacaatca
     1441 atttccggaa catttttatg acgatagcat ctttgaatta ccgcgcataa cactagatag
     1501 tttaaagtga gtcagtaatt acgcctatcc atggaatatc tcagaataat tggcggtacg
     1561 ggattaagcc ttatttttct tgccattgtt ttcattccca tggaaaaagt attccctgca
     1621 aaaccgggac aaaaaattct cagaccttat tggaaaacgg atttgtgtta ttttttaggg
     1681 caatatttat tatggggatt tttggtattg tgggcaatga tgcaattgag ttttttcctg
     1741 gatgatattg ttccgcattc atttcgtact gccgtacaat tacaaccaaa atggttgcaa
     1801 attattgagg tggttttagg tagtgaccta ttgatttatt ggggacatcg actgcaacac
     1861 cgcatagatt ttttgtggcg atttcacaaa attcatcaca gtgcagaaca tctcgattgg
     1921 ttggcggcgc atagagaaca tccattagat agtttgtata ccattggatt gattaatctt
     1981 cctgcttttt tgctcggatt tcatttagac ctgctagccg gattaattgc attcagggga
     2041 atatgggcga tctatattca ttcaaatgtg cgacttcgac tcgggccttt acgtgttttg
     2101 ataggatcgc cggagttgca tcactggcat catgatctgg atcgcgacag ggggaattat
     2161 gccaatattt ctccattgat ggatgtcatc tttggcacac acgtatttcc ggatcacgag
     2221 cctgaacatt ttggcatcaa agaggaattt ccaaagtcat atccggcaca gctcctggca
     2281 ccatttaaaa gaactaaata gtatttacaa tctgtcgcaa gctaaaatct attgtattta
     2341 ttaccttcaa taattattgt taaggcctgt catcaaatac cctattaatt gttggcaaag
     2401 cgtaatttag ccgcataaca tcttttttat gttcacatcc acaaagaaaa aactcctcac
     2461 cctgtcagct tgcgtatccg tgtttgcata tcagcatgcc gatgcccaaa ttgatgccgg
     2521 actattcaga tttcctgatg tgtccgctac acaaattgta tttacctatg ccaatgatgt
     2581 atggatcgca cctattgaag gcggaacggc tattaaatta agttctcctt caggagttga
     2641 aagcttccca aaattttctc ctgatggaaa aacaattgct tatacagcaa attacgatgg
     2701 caactgggat gtcttcagca taccaactac gggtgggctg ccggcgcgac taacccaaca
     2761 cggctggacc gacagggttg ttgactggac acccgatgga aaatcggtat tgtttgcgtc
     2821 cgggagggag agtgaaaagg agcgttattc gaaattattc accataccgg cacaaggtgg
     2881 tgcgcctacc actttgccat tagcttatgg tgagtttgcc agttataatg ccgatggttc
     2941 aagcatggca ctggtttttc gttcagaggc gttccgcacc tggaaacgtt atcgcggcgg
     3001 tgatgttgcc gatatctaca tatttaattt tgctgataat agttcctata atatttcagg
     3061 cgatattgat gcaggtgaag aatttccaat gtggtatggc aatgccattt attttctatc
     3121 agataatggc tctgaaaaac gcatgaatat ctggaagtac gacatggcta caaaatcgcg
     3181 tacgcagatc acgcatttca ccgattatga tgtgcatttt ccatcaatgg gtcctaatga
     3241 tatcattttg gaagcaggtg gaaaaatgta tttgtatgga ttgcaatcgg gtaatttgcg
     3301 tgaagtaaaa cttaatgtag ttacggataa tacaaacctg aaaccacata ccgaaaatgt
     3361 tgccgcgctg atgcagaata tgcaaattgg tcccgatggt aatagggtag tggttgaagc
     3421 gcgcggtgaa ttattttcta ttcccgcaga aaacggatat gtaaaaaacc tcactcgcaa
     3481 tagcggaact gcagagcgct atcctgcttg gtcgccaaat ggaaaatgga tagcctattg
     3541 gagcgatgca tccggagaat atgaattgtg gaccatggat atggaaaaca atggcgcaac
     3601 aaaacaaatt acacatttgg gaaaaggcta tcgatattac atatactggt cgcctgatag
     3661 caagaaggtt gtatttgttg atcaggccat gaaaatctgg atggttgata tggctaccgg
     3721 tgttgcaaca aatgtagatc atggtcttca gcaggcgcat tataatttaa atggatttac
     3781 cgcaaactgg tcgcccgata gcaggtatat tgcttacttg cgcgatctgg ataatgcata
     3841 ttatgcggca tttatatacg acacgcaaga taaaaccatt catcaggtga cgagtggatt
     3901 ttataattgc tcggaagtaa catttgatcc ttccggaaaa tatttatatg ttgcaacggg
     3961 gcaatcgttt tcaccgacat acagcgatct cgacaattcg tttatttatc ctaacacaac
     4021 cgaactagct gccattagtt tgaaaaaaga tacgccatcc ttattggtgg cgaaaaacga
     4081 tatggtggag ataaagaaag aagaagcgaa agaagaaaag gctgatgata agaaagacaa
     4141 aaacaaaaaa gataaatctg aaaaagaaga agactccgct tcaacaattg cgcctaccga
     4201 aattgattgg gatggtatag aaggcagaat ggtactattg cctattcctg caggaaatat
     4261 gtataatgta tccggagttg aaggaaaaat aatatttatt cgatatccca atacaggtac
     4321 tgctgaagga agcacaccaa gtttaaaata ttatgatatt gaaaagcgcg aagagaaaac
     4381 tattctcgat ggtgtatggg attatgcgct tactgctgat ggaaaaaaag tattggtgat
     4441 gggcgatggc atgcaagcga tagtagcagc tgaagagggt cagaaatttg aaaaaccatt
     4501 gcgcattaat gaaatggaaa tggtagtaga tcccatggcc gaatggaagc aaatattcat
     4561 ggatacctgg cgcatagaac gcgattattt ttatgatgtg aatatgcacg gcgttaattg
     4621 ggaacaagta aaagagcgtt atctcaagat gttagccggt gcaactacgc gcgaagaggt
     4681 gaatgttgta ttgggcgata tgattgccga attaaacagc tcgcatgcat atcgtttcgg
     4741 tggcgacctg gaagcaacac caatggctaa ttgcggatat cttggtgtta actggacagc
     4801 agacggacaa tattatcgcg ttggaaaaat aattcgtggc gcatcgtggg atgcagaaat
     4861 gcgttcgccg cttgatgcac ctggaatagg tattgttgaa ggagattata ttcttgcggt
     4921 aaacggaaat cccattctca ccacacaaga accatttgca tatttccaag gccttggtgg
     4981 aaagacagta gaacttacat acaacaataa gccaagtatg gatggcgcaa aaacggttgt
     5041 tgtaacaaca ttggattacg aagggcgtct gcgcaatctt gcctggatag aacaaaatag
     5101 aaagcgcgtg gaagaagcat ctaatggtca ggtaggatat atttatgtgc ctagtaccgg
     5161 tgtcgacgga caaaatgaat tgattcgtca attcagggcc caatgggata aaccggcatt
     5221 gattattgat gaacgattta ataacggcgg acaaattccc gatcgcttta ttgaattatt
     5281 agatcgcgat ccgctggcat attgggctat tcgcgatgga aaatcttggc aatggcctcc
     5341 gttagcgcat tttggaccca aagtaatgat gatcaatggt tggagtggct cagggggtga
     5401 tgcattccct gatttcttca gacagaaaaa tttgggacca ctggttggac aacgcacctg
     5461 gggtggtttg attggtatca gcggtgcacc aacattgata gatggtggcg agattacggc
     5521 gccaacgttc agaatgtatt atcagaatgg tacatggttt gcagaaggcc atggtgttga
     5581 tccggatatt aaagtagaag aagatcttac atcacttgca aaaggtaaag atgctcaatt
     5641 ggagcgtgcc attgaagaag caatgaaatt gataaagact gaaggatatc aggcgcctgc
     5701 gcgtcctgct cccgaagtaa gataatctgc aaatattacg agcgactgac aattgaattg
     5761 attcaactgt cagtcgcttt tttatttgtg tcgtatcttc atttagaatt ttagtacatg
     5821 cgaaaacaaa ttccgctctt catttttgtt ttgttagcag ggtcgatgtc tgcacagatt
     5881 acaaaaagtg cattttttgt cggcaatagt tatactgcaa cgaataattt gccacaatta
     5941 atttcagatg ttgctgaatc agcaggtgat gtattactat ataatagtaa tgcccccggt
     6001 ggatatacat tcgaaatgca taccaccaat accaccacgc tcgaacaaat tgcattaggc
     6061 acatgggatt atgtggtgtt acaagaacaa agtcagctcc catcctttcc aatagaccag
     6121 gtggaagtag attgttttcc ttttgcaaca acgttggata gtttaatcag ggtggcgaac
     6181 acatgcaccg aaaccatgtt ttttatgact tggggaagaa aggatggcga tgcatcgaat
     6241 tgtgcattct ggcctccggt gtgtacgtac aatggcatgg atagtttgct gcacgaacgc
     6301 tatatgacaa tgggagaaga taatgatgca gaggtctctc ctgtaggtgc tgtctggcat
     6361 tatttgcgcg atacacatcc tgaaatcgaa ttatatattg gcgatggcag tcacccttcc
     6421 gcagcaggat cttatgcagc agcatgtaca ttttacgcag ccatttttag aaacgatcca
     6481 acattgatca catatgattt tacgctccct gctgatcagg cacatattat tcgcgaggca
     6541 acgaagttgg tggtttacga tagtcttgct tcatggtata taggcacata tatacatgag
     6601 ccaattgcaa attttaatgt gaataccggt atccccttta ccgcaagttt cgaaaatctg
     6661 tcggagaatg ccattagtta tttatggaat tttggtgacg gtacaaattc aacagaggcg
     6721 gcgccggtac atatttatgg agcagagggt atgtatatcg taagtttaga agttacaggg
     6781 tgttcagacg ttgatgatta tacaagcgat attacaatta ccggcgtaga aacttctatt
     6841 cagaataatg cgataataaa ctggcaaccc gaaaatccgg tgcatgctga attattttta
     6901 ccggcaaata atagcaattg gcgcattgag atattggata tggccggcaa tgtggtaatg
     6961 caaacctatg cacaaacatt aattacggtg gatggcattc acgccggtgc ttatatcatt
     7021 tatttacaac agggcgacga gcgtatgtat cgcaaactca tgattcaata atattattca
     7081 ttacccgtag tagcgccaac aatattccca tgcacgctta caaattgcca gtcatcggga
     7141 tatgggcatt gaattttgct ttttgttccg gctattaata tttttttatc gaagctttta
     7201 atattcactg taccatcggt atgtccgcca tcagcggttg acattgcttt ggtgatagtc
     7261 cagctgcctg aagcggtcgt aaacgatttt aatgcagtat cgcaatcgtc gttttcgcta
     7321 cccatttttg taaaaagcgc gagatattcc ggtttccatg ccggtaaata cggcactccg
     7381 aaatatttat tgtgtttttt atcaggacag ggttgcgtgc cacagcaagg ttcgttgcat
     7441 gtagcgccgt aatagtcgtt ggtatgtatc caaatttttt catctgagcg caatggataa
     7501 cgaaaagaag aatggaaggc gtcaacaaat tcattattaa tatgatcgtt gtgataatgt
     7561 ggtacaataa attgtaataa tacactatcg cgattgcgac caaatatttt tgtgattact
     7621 gaatctacct gttcaacatc aacagttgcg ggacctgttc cgtgcatatt gtcgccgaga
     7681 taaaaattat tgtcgttgca atccccgcaa ggatcaacaa aatcgccata accggaacca
     7741 tatatccaaa tggtatcgcc aaggcccttg aaaacaaaaa tattggatgt ggtccagggt
     7801 ttttcatcag catagatcat gctgtgtaca acatagacat cattttcaat gagtgtttcg
     7861 gtgccatcgg taagcggtcc cggatctttt ctgtgcttac atccgataat acctgccagc
     7921 accaggcaag cgatgagaag gcgttgaacc atcaggaaat tttatcaaaa ccggtgtaag
     7981 gcaccaatac ttttggaata tcaattccgc cttctgtttg attattttca agaatagacg
     8041 ccaaaatacg tggtaaggcg agtgctgagc cattaagtgt atgtacgagt ttatttttac
     8101 catcgctgct atccttgaaa cgacatttca tcctattagc ctggaaggat tcaaaattgg
     8161 aaacagagct cacttccagc cagcgttgtt gagcgccgga ccatacttcg aagtcgtatg
     8221 tgatagcaga agcaaagccc atatcgccac cacaaagtcg cagaatgcga taaggcagct
     8281 cgagcgattg cacgagtttt tccacgtggg caaccatgac atcaagacgt tcggcagaag
     8341 tattgggatg tgcgatttcc acgatttcca ccttttcgaa ctgatgcagg cgatttaaac
     8401 cgcgtacatc tttcccatag ctacccgctt ctcgacggaa acaagggctg taggcggtca
     8461 tttttatagg aaggtcctct tcttttacga tagtatcgcg atagatatta gttactggca
     8521 cttcggcggt gggtatgaga tagaagtcgt cttcggtggc atggtacatt tggccctctt
     8581 tatcaggaag ctgtccggtg ccgtatcccg aatcggcatt taccatgaaa ggaggcacaa
     8641 cttccatata acccgctgct gctgcctgat ccaggaaata ttgggtcaaa gcgcgctgta
     8701 aacgggctcc tttgcccaaa taaacaggaa aaccgcttcc ggtgatcttg ttgcccaact
     8761 caaagtcgat aagtcggtat ttagccgcaa gctcccagtg tggaagggct tcaaaggaaa
     8821 actgaggttt gttgcctccg ccgcgcactt ccaggttgtc ttcggcatct tttccttgtg
     8881 gtacaaggct ttgaggcagg tttggcaggc tcaggaggat gtttttgatt tgctcctgga
     8941 tgtcggcatg accggtctca agggcggcgc ttcgctgttt aagatctgct acgtgttgtt
     9001 ttttagcctc agcctcatcg cgcagcccct ggcccatcag cttaccgatc tctttggagg
     9061 tgctgtttgt ttcggcaagc gtgttatca
//
LOCUS       DAOQHY010000131         8675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_4113_length_8675_cov_5.875638, whole genome shotgun sequence.
ACCESSION   DAOQHY010000131 DAOQHY010000000
VERSION     DAOQHY010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8675
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4113_length_8675_cov_5.875638"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2388)
                     /locus_tag="PLL28_10700"
     CDS             complement(<1..2388)
                     /locus_tag="PLL28_10700"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HNF69837.1"
                     /translation="MKSFKSINNLTGWIVFGIALFVYASTMEATASFWDCGEFIAATY
                     KLQVVHPPGAPIFLMLGKIFSLFASTKEQVPVMTNFFAALSTSFAILFTFWIITRLIK
                     RIMSPNDEPTFSQTIVIILSGVIGALVCLFMDSMWFSAVESEVYALATFFFVIIFWSM
                     VKWEAHAGDPNSDRWILFMALLMGLSIGVHLLALLVVPAIALIYYFRNYKYTFKGLMY
                     ALLIGAGLVAFLLYGLLDKFIGLGAWMDRVFNNFGAPFGTGLIIYGIMIVGGVVWGIR
                     KAIEKSNRVLYLALMSFTFLTIGLTSYAMVLIRAEANPVINMNGINDVHSFLSYLKRE
                     QYGSRELLYGPFWTAQPVEYEKGKAKWGKVEGNDKYQIISHEYKPVYKIPSEYANSPE
                     AQLLIQRNRQILFPRMGSLEDRHAGLYYNFANVPQGQEETYVPTGGDNMRFFANYQLG
                     HMFWRYFMWNFSGRQNDSQGNFYDLYKDGNWVTGITAIDKSKNPQLVDVPAELSDIDS
                     HNVYYMLPFIIGIIGMVYQLRNDKRGFAIVFMLFLFMGFMNIINMNQPPTEPRERDYA
                     QIGAFFAFALWVGLGLAGIVDMAKNFASKKVLQEYALYSLLILLVMYFTGLTMYKFTT
                     IAYVMIYTVGFMFVVGFIGSLIYKATKKELTVGIVAFLMLAPAPYLMATQNWDDHDRS
                     DRTFARDIARNYLESCPPNAILFTQGDNDTYPLWYAQEVEGIRTDIRIVNLSLLGVDW
                     YINQLRNATNDSKPIALTLGPEKILGDKRNQIVSNDKSKFKNTTMDINDFLNFVGQ"
     gene            2520..4202
                     /locus_tag="PLL28_10705"
     CDS             2520..4202
                     /locus_tag="PLL28_10705"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HNF69838.1"
                     /translation="MGLNIGLLLGGIGVFVFGIQLLEKALRSLMSRRFKLFLRRQTGR
                     PFRAVFGGTVLSAALQSSSVVNFLVLAFAGSGIISLKNALAVIMGTNLGTTLNSWIVA
                     TIGFRLEIEMYALPVAGVAGLLLFVFPEKKFSSIARFFMGFSFLFIGLGFMQQSVQDE
                     AIIQAMSRFRDSGAFVFLILGFIATTITQSSAATVAITLSLLYASAVGFSDAMAIVIG
                     SEVGTSVKLILGALDGVPVKKRISVGNFMYNLVTTIVAFALLRPAEICITEIMHVRDP
                     LIGLAVFQTGMNLMSIILFFPFLSAFSNRLEKSFNTEPAIASLFITPHTPVIPDGGLE
                     LLKKETGYFIHACMSFNAHNFHLHDTEIPMMPEFAEILHQRNTEQQPLALRYMLLKEQ
                     YGEIQSYYIRLKSQNLEPDDAIMVEQLIAGVRSAMYASKCIKDLFDDLHEWQNSADEQ
                     QYAFLKNMRIDVRHFYMQLTLNKWQSAEGDVVSDELLALLKHAQQTYQHNIEDIYRSM
                     SEHHISDTELATRMNFNRELFTSSKSMIMSCRSYLLDPLRAEKFNEIPTYIP"
     gene            complement(4364..6079)
                     /locus_tag="PLL28_10710"
     CDS             complement(4364..6079)
                     /locus_tag="PLL28_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF69839.1"
                     /translation="MAQPAIKTNYGALSTLVTVFFFWGFIASGNGVFIPFCKHYFHLD
                     QFQSQLIDFAFYFAYYIGALLLFFFSTAGNNDLVAKWGFKKSIIYGLLFSFLGSLAMI
                     AAVNLGGFAAILISLFIVALGFSLQQTAAQPFAISLGDPATGNTRITLGGGINSFGTT
                     IGPIIVGFALFGTAAVSDDMINNLGLGSVTFLYVGIGALFLLCAALFRMSKKVPDGKM
                     ESHIEHAHKALTTMMVITAVIFIIFPIVFASYNSADAVKVEEMKTALAAIPETDTAAI
                     ATANTAIEELNAPIEQNRMILLSLALISVAGILLFAKSRATKNPNGWGALQYPQLVLG
                     MLAIFTYVGVEVSIQSNLGELLAQKAFGGYKASETAPFISIYWGSLMIGRWVGSVAVF
                     SMSDARKKLMMIIVPIIAFGVIIGVNSIVGHDMRMFYAYIVCIAVQIAGFFLGKDKPA
                     RTLLIFSVLGITSMIVGLMTTGNTAIFAFLSGGLFCSIMWPNIFALSLTKLGKYTSQG
                     SSLLIMMILGGGIIPPIQGKIADIIGIHSSYFLPALCFTYLAWFAWRMRNLLGENETV
                     TAGGH"
     gene            complement(6149..6646)
                     /locus_tag="PLL28_10715"
     CDS             complement(6149..6646)
                     /locus_tag="PLL28_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69840.1"
                     /translation="MKRSLPFLLLPLMWVLAGCPVSVDYPLGYQDKDKIDNALIGTWA
                     AADTSAEVQEVKISKIDEFTLAVEVTKRGSMYVEETDFFKGWCTTINNQKFVYFQNAG
                     DNTQGYYSYNYWFEGKQLMTSDFALKVGGVDAVTSIEAYRQEVKASMEYEDFRGTVFA
                     YNKTR"
     gene            7333..7890
                     /locus_tag="PLL28_10720"
     CDS             7333..7890
                     /locus_tag="PLL28_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNF69841.1"
                     /translation="MAYITLPEGVPGIIGPMMFRPETAKPMNELVEVLLRGPHSLSSG
                     ERELIAAFTSYRNKTLFCYRSHGASAAHHLNGNIGLVYQVLEDYHRADISDKLKSLLA
                     IAEKVQMDGKLVAQQDIDNAKQHGATELEIHDTVLIAAAFCMYNRYVDGLGTFQPAEI
                     EAYRETGRMLAEDGYISAIPEPQTV"
     gene            7972..8166
                     /locus_tag="PLL28_10725"
                     /pseudo
     CDS             7972..8166
                     /locus_tag="PLL28_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118476.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
BASE COUNT         2617 a         1751 c         1831 g         2476 t
ORIGIN      
        1 ctgaccaaca aagttgagga aatcattaat atccattgtt gtattcttga acttactctt
       61 atcgttggat acaatttgat ttcgtttgtc acctaaaatc ttttcaggtc ctaatgttaa
      121 tgctatcggt ttagaatcgt tcgttgcatt gcgcaattgg ttgatatacc agtcgacacc
      181 aagcaagcta agatttacga tgcgaatatc tgttctgatt ccttccactt cctgagcata
      241 ccacaatgga taagtatcat tatcgccttg tgtaaacaag atggcattcg gagggcatga
      301 ttccagataa ttacgcgcaa tatcacgagc aaaagtgcga tcgctgcgat cgtgatcatc
      361 ccagttttgt gttgccatta aatatggcgc aggagcaagc atgaggaatg caacaatgcc
      421 gacagtgagt tctttttttg ttgccttata aatcagcgag ccgataaagc cgacaacaaa
      481 cataaagcct acagtatata tcattacata agcgatggta gtgaacttat acatggtaag
      541 tcctgtaaaa tacatcacca ataaaatcag taagctgtat agtgcatatt cttgcaatac
      601 ttttttgctg gcaaaattct tagccatatc cacaatacca gccaggccta atccaaccca
      661 cagtgcaaaa gcgaagaatg caccaatctg tgcatagtcg cgttcacgtg gttctgttgg
      721 tggctggttc atgttgatga tattcatgaa tcccataaag aggaataaca tgaatacaat
      781 agcaaaacct cgtttgtcat ttctcaattg atacaccata ccaatgatac caatgataaa
      841 cggcaacata taatatacgt tatgcgaatc gatatcactt aattctgcag gcacatctac
      901 taactgcgga ttttttgatt tatcaatcgc tgtgattccg gttacccagt ttccatcttt
      961 atataaatcg tagaaatttc cttgtgaatc attttgacgt cctgagaaat tccacatgaa
     1021 atatcgccag aacatgtgtc cgagctgata attagcaaaa aaccgcatat tgtctccacc
     1081 tgtcggaacg tacgtttctt cctgaccttg aggcacattg gcgaaattgt aataaagtcc
     1141 ggcatgacga tcctccagtg atcccattct tgggaatagt atctggcggt tgcgttgaat
     1201 cagcaattgt gcctcagggc tgttggcata ctctgacggt attttatata ccggcttgta
     1261 ttcatgagaa ataatttgat atttatcatt tccttcaacc ttaccccatt tagcttttcc
     1321 tttttcgtat tcaacaggtt gtgctgtcca gaaaggtccg tataacaatt ccctgcttcc
     1381 gtattgttcg cgtttaagat atgatagaaa tgagtgcaca tcattaatac cgttcatatt
     1441 aataacggga tttgcctcag cgcgaatcaa caccattgca taagaagtca aaccaatcgt
     1501 aaggaaagtg aatgacatca aagcgagata taatacgcga ttgctttttt ctatggcttt
     1561 gcgtataccc caaactacac cgccaacaat cataattccg taaataatga gaccggtgcc
     1621 gaatggtgct ccgaagttat taaacacgcg atccatccat gcgcctagac cgataaattt
     1681 atcgagcaga ccataaagga gaaatgccac taatcctgca ccaatcaata aggcatacat
     1741 taaacctttg aaggtgtatt tataatttct gaaataatat atcaatgcga tagcaggaac
     1801 cacaagcaag gctaataagt gtacgccaat agataatccc ataagcaagg ccatgaataa
     1861 tatccagcga tcagaattag gatcaccggc gtgggcttcc cattttacca tcgaccagaa
     1921 aataatcacg aagaaaaatg ttgccaatgc gtaaacttcc gattcaacag ctgagaacca
     1981 catgctatcc ataaacaaac aaaccaaagc accaataaca ccgctgagga tgatgactat
     2041 agtttgagaa aatgttggtt catcatttgg gctcataatt ctttttatga gtcttgtaat
     2101 gatccaaaaa gtgaataata tggcaaagga tgtagaaagt gcggcaaaga agttcgtcat
     2161 taccggcact tgttctttcg tagaggcaaa aaggctgaaa attttaccta acatcaaaaa
     2221 gattggcgct cccggcgggt gcactacctg tagtttatac gttgctgcaa taaactctcc
     2281 gcaatcccag aaactggcgg tagcttccat agtactggca tacacaaaaa gtgcgatgcc
     2341 aaatacgatc catcctgtaa ggttgttgat acttttgaaa ctcttcatgt aagtgctgat
     2401 tagcggcggt aaagatatat aatatgccct attgaagcct gtttaaagct gttggcaatg
     2461 cgccttgtca ttgtttttag cggcattgtt aactaagttt aaccctgtaa cagccattta
     2521 tgggattgaa catcggttta ttgctggggg gaattggggt ctttgtgttc gggatacaac
     2581 tgcttgaaaa ggcgcttaga agccttatga gccggcgttt taagctgttt ttgaggaggc
     2641 aaaccggcag acctttcaga gcggttttcg ggggaacagt attgtcggcg gcattacaaa
     2701 gcagttctgt ggtcaatttt ctggttctgg catttgcggg aagtggaata atctccctga
     2761 aaaatgcgct ggccgtgatt atgggcacta atctgggcac taccctcaat agctggatcg
     2821 ttgccaccat cggatttcgc ctcgagattg aaatgtatgc cctccccgtt gccggagtag
     2881 cgggattgct gctatttgtg ttccctgaaa agaaattttc gtcgattgcc cgatttttta
     2941 tgggcttcag ctttttattc attggtctgg ggtttatgca gcaatcagta caggatgagg
     3001 ctattattca ggcgatgtcg cgcttccggg atagtggagc atttgttttt ctgattttgg
     3061 gcttcatagc aacaaccatc acgcaatctt ctgccgcaac agttgctatt actttgagtt
     3121 tattatatgc ctctgcagtc ggctttagcg atgccatggc aattgtaatt gggtcggaag
     3181 taggaacttc tgtgaaatta attctcggag cattagatgg tgtaccggtg aaaaaaagaa
     3241 tttcggttgg caatttcatg tataacctag tgactacaat tgtcgcattt gcattattgc
     3301 gaccggcaga aatatgtatt acagaaatca tgcacgtgcg cgatccatta atcggattgg
     3361 cagtatttca aacagggatg aatttgatga gcatcatttt attttttccc tttctgtcag
     3421 cttttagcaa taggcttgaa aaatcattta acaccgagcc ggctattgct tcattattta
     3481 ttacgccgca tacaccagtt attcctgatg gagggcttga actattgaag aaagaaacgg
     3541 gatatttcat acacgcatgc atgagtttca atgcccataa ttttcacctg catgacactg
     3601 aaatacccat gatgcctgag tttgccgaga ttcttcatca gcgtaatact gaacagcaac
     3661 cacttgcatt acggtacatg ttgttaaagg aacagtacgg tgaaatacaa tcttattata
     3721 tcagattaaa atcacaaaac ctcgaacctg atgatgctat catggtagaa caactgatag
     3781 ccggagtgcg cagtgccatg tatgcttcaa aatgtatcaa agatttgttt gatgatttgc
     3841 atgaatggca aaattctgcc gatgaacagc agtatgcatt tctaaaaaat atgcgcattg
     3901 atgttcgtca cttttatatg caattgacat taaataaatg gcaatcagct gaaggcgatg
     3961 tcgtttcaga tgaattatta gcattgttga aacatgcaca acaaacatat cagcataata
     4021 tagaagatat ttatcgcagc atgtcagaac atcatatcag tgacacagaa ctggcaacgc
     4081 gcatgaattt taatcgcgag ttattcacct cctcaaaatc aatgatcatg agttgtcgca
     4141 gttatctgct cgatccgctg agggcagaaa aattcaatga aattccaacc tatattcctt
     4201 agcactcatc tcaaaaatgg gtttgaatta gaattttaag tattcgcact ttagccaagg
     4261 cacttgaagc agcccctact aacggtatta cgggcaattc aagtaacaaa ggatgaaggg
     4321 cgaagacaca gaaaagacgc caaatatatt tttgagatga gttctaatgt cctcctgccg
     4381 taacggtttc gttttctccc aacagattgc gcattctcca tgcaaaccag gcgagatatg
     4441 tgaaacataa tgccggcagg aagtagctgc tgtggatacc gataatatct gcaattttac
     4501 cttgtattgg agggatgata ccgccgccaa gaatcatcat gatcaataat gaagaacctt
     4561 gagaagtata ttttccaagt tttgtgagcg acaatgcaaa aatattcggc cacatgatac
     4621 tgcagaaaag tccgccactt aagaatgcaa atattgctgt gttacctgta gtcatcaatc
     4681 cgacaatcat tgaagtgata cccagcacac tgaatattaa taatgtgcga gcaggcttat
     4741 cttttccaag gaagaatccg gcaatttgaa cagcgataca aacgatatag gcataaaaca
     4801 ttctcatgtc gtgaccaaca atgctattta caccaatgat gacaccaaat gcgatgatag
     4861 gaactatgat catcatcaat ttttttcgcg catcagacat ggagaaaaca gcaacagatc
     4921 cgacccatct gcctatcatt aagctacccc aataaatgga aataaatggt gccgtttcag
     4981 atgctttgta tccgccgaaa gccttctgtg ccaatagttc accgagatta ctctgaatgg
     5041 aaacttccac gcctacataa gtaaatatcg ccagcatgcc cagtactaat tgaggatatt
     5101 gcaatgcacc ccaaccgttt ggattttttg ttgctcttga ttttgcgaat aacaaaatac
     5161 cggcaacaga aattaaggcc agtgataaca aaatcatcct gttttgttct atcggcgcat
     5221 tcaattcttc aatagctgta tttgctgttg cgatggcagc agtatcggtt tcaggaattg
     5281 ctgccaaagc agttttcatt tcctctactt taaccgcatc agcactatta taactggcga
     5341 aaacaattgg gaatatgatg aatatcacag cagtgatcac catcatagtt gtcaatgcct
     5401 tgtgtgcatg ttcgatatgc gattccattt ttccgtcagg cacttttttc gacatcctga
     5461 ataatgcagc acataacaga aaaagagcac cgatgccaac gtataagaat gtaaccgaac
     5521 caagtccgag gttattgatc atatcatcgc tcactgctgc tgttccgaag agcgcaaaac
     5581 caacaatgat aggtcctatg gttgtgccga aggaattaat accaccgccg agggtgatac
     5641 gcgtatttcc ggttgccgga tcgcctagag atatggcgaa aggttgggca gcagtttgtt
     5701 gcaaagaaaa tcccagtgcc acaataaata agctgatcag gatggctgca aatccaccta
     5761 agttcacagc agctatcatg gccagagatc ccagaaaaga aaacagcaat ccgtaaataa
     5821 tactcttttt aaagccccat ttggctacca ggtcgttatt tccggcagtg ctaaaaaaga
     5881 acaacaacaa tgcaccaatg tagtaagcaa aataaaacgc aaagtcgatc aactgacttt
     5941 gaaactgatc gaggtggaaa taatgcttac agaatggaat aaatacgccg ttgccggatg
     6001 caataaatcc ccagaaaaag aaaacggtaa caagggtgct taaggcacca taattagtct
     6061 tgattgcagg ttgagccatt gatacgcctg tttgtggcgc aacaaataag catataattc
     6121 ttcaaattgc caaagaagat attgacaatc agcgtgtttt attataagca aagacagttc
     6181 ccctgaaatc ttcatattcc atggaggctt ttacctcctg gcgataagct tcaatagatg
     6241 taacggcatc aacaccgcca actttaagtg cgaaatcgga ggtcatgagt tgcttgcctt
     6301 caaaccaata gttataagag tagtagcctt gggtattgtc gccggcattt tggaaataca
     6361 cgaatttctg attgttgata gtagtgcacc atcctttaaa gaagtccgtt tcctcaacat
     6421 acattgatcc gcgtttggtt acttctactg cgagtgtgaa ctcgtcaatt ttagatattt
     6481 tgacctcctg cacttctgct gaagtatcag ctgctgccca ggttccgatg agtgcgttat
     6541 cgattttgtc cttatcctga taccctaaag ggtaatccac agacacagga cagccggcca
     6601 aaacccacat gagggggagg aggagaaagg ggagtgagcg tttcatggag taaaaataat
     6661 aaaataggag agaggagaga ggagaaagga gagaggggtg agtgggtgag tggggcagtg
     6721 ggtcagtggg tgagtggtga gtggggagtg gggagtggtg aaattaaatg ctctgccgaa
     6781 ggcagagcgc tatgatcaat ttcaagggcc tgccctgaag tgacaaaata tgcagcgaag
     6841 cggaatattt tttctacttc agggtttggg tagagatgtc attatagatt aagctttgta
     6901 gttgccccgg aagtagacaa ttttctctgt tgcattacga aaatttacaa ttcggggtag
     6961 gctatttgtt ttattcaaaa cggtctgcct acggtagaca acttcctgac gcccacaggg
     7021 ttcttgcatt tgtctcacaa ctcaataccc actactcact accaatggtg agtggtgaat
     7081 ggtgagtggt gagtgggtga gtgggtgagt ggtgaatggt gagtcgtgaa ttttacctga
     7141 tacttgatac ctgatacctg atacaaattc tagatactaa aaccaatcat gcatccggtg
     7201 ccgatatgtg atttattaac taaacgaagt gtaaattttg ttaaagtagt ttgaaacaat
     7261 taaacatagt gtaaatggtt tggcagcatc aatggagttg gaatattttc atccgttcaa
     7321 agaaaaacaa ctatggcata cattactcta cccgaaggcg taccgggtat tatcggacct
     7381 atgatgttcc gtcccgaaac cgccaaacca atgaatgaat tagtggaagt gctgttgcga
     7441 ggtccacatt cactttcaag cggcgagcgc gaattgattg ctgcttttac ttcgtatcgc
     7501 aataagacat tattttgtta ccgttcacat ggggcctctg cggcgcatca tttgaatggt
     7561 aatatcggat tggtgtatca ggtgcttgaa gattatcacc gtgcagatat ttccgataaa
     7621 ttaaagtcct tgttagcgat tgctgaaaaa gtgcaaatgg atggaaaact tgttgcacag
     7681 caggatattg ataacgcaaa acaacatggt gctactgaat tagaaattca cgacacggta
     7741 ttgattgctg ctgctttctg catgtataac agatatgttg atggtcttgg tacttttcaa
     7801 cctgcagaaa tcgaagcata ccgggaaaca ggacgcatgc tggcggaaga tggctatatc
     7861 agtgccatcc ctgaaccaca aacggtataa acaattttta ttacaggcag aggttaagcc
     7921 gatgtacgcg agtatgtcgg ctttttttat ttttataccc tgatagccct catgaatact
     7981 ttatatgata ttttagaagt ttcaaagcat gctactgccg acgagataaa aaaggcctac
     8041 cgtcggttag caaaaaaata tcatccggat gtaaatcccg gcaataaatc ggcagaagaa
     8101 aaattcaagc agttggcgaa tgcttatgag gtattgtctg atcctcgcaa acgagcatcc
     8161 tatgattata tcacatatca ctaccagcat gcaggaccta tgcatggccg acccaacagt
     8221 caatcgactt cgcaacgcac cggctcttca acgtatgatc gcggtccggt cgacaacagc
     8281 aatagaaatg atcgaagggt agcgaatttg atatttgttg tcggactcct catttttaat
     8341 ctcattatcc aaaaagactg gagtcctgat agggattatc attttaaatc aataaatata
     8401 gattctctgc ttaaatacaa ttccacgtcc gattctatat ccgaatctcc atattttcca
     8461 ttacttggtg atactattac gatattgaaa gagagggata gtatttgatt tagggcaaat
     8521 taatggaatc agcttggctt attgttagat tgttgtttgg tccccggaaa cctttaactg
     8581 aacatagatt gcgccaggct tgcattaatt ctgtataatt acttccgact ttgatgttga
     8641 tgcaagtcta tgttatggtt caactattca ggatg
//
LOCUS       DAOQHY010000132         8535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_4214_length_8535_cov_6.466745, whole genome shotgun sequence.
ACCESSION   DAOQHY010000132 DAOQHY010000000
VERSION     DAOQHY010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8535
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4214_length_8535_cov_6.466745"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..844)
                     /locus_tag="PLL28_10730"
     CDS             complement(197..844)
                     /locus_tag="PLL28_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69842.1"
                     /translation="MKLFSLISAIIFCTSAVIAQTDGQISSCYLFVDLYNNSATGFSE
                     YRKDMPDTAAEFELDDILFTAYQDLELGTGMIYKTPHQTSTIFQNYYYTKWEFNVEGK
                     ALYPNVLDQPWEVNKVEIEPYFTTLCNWIHDNCVGQLQMSPIYDVSALPNSFGVDKLT
                     CYLYHPAITLPEGLTTEQSIQMLEQVPSIKINVYENWFPKGTYRMEYSVIGIQQE"
     gene            complement(854..1570)
                     /locus_tag="PLL28_10735"
     CDS             complement(854..1570)
                     /locus_tag="PLL28_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69843.1"
                     /translation="MRSITLLAIITFLGITPAKSQSYGYGGIGYNMSFLQSDGLDFVI
                     GRYNDTRAYLTETMENPRYFDGISIHFGGAQKAFLFDFGYTQQSCVVSASGVDQSGIE
                     QQRDVKDKWNTIDIGLGVSAGASDHFSLAAGVNFGLNSEKYLTRAETPDNIGTANFAQ
                     INKQFKFGISPFVQFTITGESAGLLIRPYYAWSPVKTDYYDLNAYINPYTYILDPINI
                     EGTLKGFGLSVLFILYEYQD"
     gene            complement(1659..3056)
                     /locus_tag="PLL28_10740"
     CDS             complement(1659..3056)
                     /locus_tag="PLL28_10740"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF69844.1"
                     /translation="MASGYAQTQILHFGSNEYALTPEMQSALNKDLEAFKQAPNISIT
                     GHTDADGSDGYNLALGEKRATSVKAYLISMGVDARNIEIYSSGESKPLGDNQTAEGKE
                     ANRRVEVVANWVANPEKNLIKPMLIDDKTSIQVPQALLPSPIYKTIDPTKANDVPIGD
                     KGTVLHIPANAFVDAQGNVVKDHVKLAFTEYKNAADMAFSEISMTYQNDGEEMRFNSA
                     GMFSLYGTSNGKDITIRDNTEINVDYALATQQHDIAFYGLQNNDTWELVENIAEMNVD
                     TLVFGLQGDSLVLLDSTRKHSDYGFRLRRFNGEKIEEEDIILWDPKTSSQMIRGVDAG
                     HQYPDIVRGLSVGDFGVYNCDQIYRIGKSITINPVFVDEHGQKISDGNILSLIDLNYN
                     GAFSFDPNAYITLDANGRNVLLLFTKSNKLYLISESEMKEQKLNNNVSHTFTMKNVTE
                     QLNSTEKLSEYLGLN"
     gene            complement(3226..4635)
                     /locus_tag="PLL28_10745"
     CDS             complement(3226..4635)
                     /locus_tag="PLL28_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF69845.1"
                     /translation="MQFTSRITSFLCISLIAISVCNAQSISLYFGVDDATLTPASIKN
                     IDEQMSKLKAAPGLRVIGYADADGVNDDNDVLSDARAAAITNYLTTKGISSDVIVAFG
                     NGETMAKQNNASAYDKQLDRRVDIEVLNTTELALAKKLSTFKTPDRFKVTPIIITFDP
                     TKPNDIPIGTRGTILHIPANSFVDASGKNVTSTVTISFSEYTTSGEMVFSGIPMHYKT
                     GTDNQRFSSSGMFKIEGTAAGAAIEMAPNASVTMDYAMVGNQNDVGFYTLDEKGEWQL
                     INNIGVDGKPIASNTSSKTGNKTQETKQVKADSATAMQFANAFNNNLSTVKTERITGS
                     TTRASVKASLTLSGFGYYNCDALAPMVARVNIGGNFLDDQGKPILRINNITVVDLKFN
                     GAFYYNNPANIQLDSRSDNMIFVSNQANRIFYVSNADMKAMKISKSGNYDIKMKEITK
                     THTNNDQLSALIQSNKMSK"
     gene            complement(4698..5210)
                     /locus_tag="PLL28_10750"
     CDS             complement(4698..5210)
                     /locus_tag="PLL28_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69846.1"
                     /translation="MLLCVITLVGRNLSAQPTPQEIRAKEADKKVEVIKASKPTDSVV
                     LAAGDPLIKDIQPQTTSLKGYWYNGKLQLCIAKSIDASGNKWEVRYYFSDTSLYQIVS
                     DFYTVQTFISPDFFVWQFKKTFYLKYGEMFYYAADGTDRGMPPEYEDLALMQAYFKKY
                     YAAMNSAHTK"
     gene            complement(5347..6138)
                     /locus_tag="PLL28_10755"
     CDS             complement(5347..6138)
                     /locus_tag="PLL28_10755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69847.1"
                     /translation="MKKHVLLLALVAGLFSVQSLTAQITGFCFTDAFGYTTSVTATKT
                     GPGHWDLSGTADVLTGSDWIVTGSYDKMSDVWTLNFANPAPDGCTFYVDNFTYTSYAY
                     GGGVIYFNWTSYCFGGALGSGSGSTTWSLGTCPMRLGNETAIVGAAAADLTIANAPVA
                     DLRSIPGGELFSSAFTEAQFSVTRNADNTFSFGYTVDTESDVAIEIYNHAGQHIATIV
                     NGHMSNGFYVNNWDGKLANGTDALSGVYIAVMKSNGNMVTNKFVK"
     gene            complement(6411..6962)
                     /locus_tag="PLL28_10760"
     CDS             complement(6411..6962)
                     /locus_tag="PLL28_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69848.1"
                     /translation="MPANQFKYLLHGIFITLLLLMTPRLNGQVFFYTNVIADIDKQVE
                     KINDRKGNNGIRIAPWDSIQNFYDFNGPNIFGHFHFNVLEKAMIKIEEESGTQWTTTY
                     YFKDNKLIHVEITCFRIAGFSGPTWYELAGKYRYWFDHDAFISSDITLIPPDSPYQVS
                     FTSAELLDQATCFADLLYKEQEK"
     gene            complement(6943..7545)
                     /locus_tag="PLL28_10765"
     CDS             complement(6943..7545)
                     /locus_tag="PLL28_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69849.1"
                     /translation="MQPLFPKSIETIIATVAGLLITSAQLILIRDVWRKKINPSLLTW
                     LGWGLIMGTGLLAQILEDGWQSSLTGLALSTAGCLCIFTIALLRNTFLYQRSDIKYLV
                     LGLLCLIIYISSHNAIITTLFAILADFIAGIPTLIAGYRNPKSQKTIAWTLGAVSWAF
                     TISVCFGHAWLYAWFPIYLFLYNLTLYIFTHRSTNAGKSI"
     gene            complement(7618..>8535)
                     /locus_tag="PLL28_10770"
     CDS             complement(7618..>8535)
                     /locus_tag="PLL28_10770"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNF69850.1"
                     /translation="EGNNLQYPNWILEKYFYEYYSYIYGHPETFQLSTTCRIDDKLFE
                     DCVLVEIPAVTKDTLVERRKKRYPDYPMTLARDKGLSFTRYDSLDAAVLSLFTWDKTI
                     LRKVYGIHFKDSINTYMQTAIDDSIGTLIIDLRNNQGGFMMYGVYVMRWLMDSTFNYI
                     DKIERVHRTSSNERIYKSVNTGRFKSVKPYKHNYGGNVIILTNGGTFSNSSIFTTQMQ
                     RYGRAVVMGEETGGSSSQLTGSFGVHRPLIMPNTKIELSHINYRIVVNKDVPYSGKGV
                     IPDIAIADDWNPENGDILLQKVLKEYLPK"
BASE COUNT         2487 a         1702 c         1707 g         2639 t
ORIGIN      
        1 attcccaccc gcctgcggcg aggctggcat tcccacattc ccacccgcct gcggcgaggc
       61 tggcattccc acattcccac ccgcctgcgg cgaggctggc attcccacat tctcacattc
      121 ccacccgcct gcggcgaggc tggcattccc acattcccac atttctcctc tctcctctct
      181 cctctttcct ctttccttat tcctgctgaa ttccaattac cgaatattcc attctgtatg
      241 ttccttttgg aaaccaattt tcataaacgt taatcttaat acttggaacc tgctcaagca
      301 tttgaatgga ttgctcggta gttaacccct ccggtaatgt aatggcagga tgatagagat
      361 agcaagtcag tttatctacg ccaaatgaat tcggtagtgc agacacatca tagattggcg
      421 acatttgtaa ttgaccaaca caattatcgt gaatccaatt gcataaagta gtaaaatacg
      481 gttcaatttc aactttattt acttcccatg gttgatctaa tacatttgga taaagggctt
      541 ttccttcaac attaaattcc catttggtat aataataatt ttgaaatata gtagaagttt
      601 gatgcggtgt tttgtaaatc atgcctgtac ctaattcaag gtcttgatag gctgtaaaaa
      661 gaatatcatc taattcgaac tccgctgccg tatcgggcat atcttttcta tattcagaaa
      721 atccggtagc agaattgttg taaagatcaa caaacaaata gcatgaactg atttgaccat
      781 cggtttgtgc tataacagct gatgtgcaaa atataattgc actgataagc gaaaataatt
      841 tcatggaaat aatttaatct tgatattcgt acagaataaa taatacagat aatccaaatc
      901 ccttcaaagt tccctcgatg tttatgggat ctagtatata agtataagga ttgatataag
      961 cattcagatc atagtagtcg gttttcaccg gcgaccaggc atagtatggt cttatcagga
     1021 ggccggcgga ttcaccggta atcgtaaatt gtacaaaagg cgaaatgcca aatttgaatt
     1081 gtttattgat ttgcgcaaag ttggctgtgc cgatattatc aggcgtttct gctctggtaa
     1141 gatatttctc actatttaat ccgaagttca caccggcggc tagtgagaag tgatcgcttg
     1201 ctccggcgct cacacccaac ccgatgtcaa tggtattcca tttgtcctta acatcgcgtt
     1261 gttgttctat gcccgactgg tcgacaccac ttgcgctaac cacgcagctt tgctgggtat
     1321 aaccaaagtc gaagagaaat gctttttggg caccgccaaa gtgaatggat attccatcaa
     1381 aataccgggg attctccatg gtttccgtga ggtaagcccg tgtatcgttg tatctgccaa
     1441 tcacaaaatc aagtccgtcg gattgaagaa atgacatgtt atagccgatt cccccatagc
     1501 cataactctg agattttgca ggggtaatgc caagaaatgt aataattgca agaagtgtaa
     1561 tcgatcgcat agcgttgttt cggttgtgtg tcagcccgaa aattacgcaa ttttgaaatc
     1621 gtcggcaaaa tgatatttat catgaagacc ggtgtcgatt aatttagtcc aaggtattcc
     1681 gaaagttttt ctgtgctgtt aagctgttca gttacatttt tcatagtaaa ggtatgagag
     1741 acattgttat tcaatttttg ctctttcatt tctgattcag aaatcaaata caacttgttt
     1801 gattttgtga aaaggagcaa cacgtttctt ccgtttgcat cgagcgtgat gtaggcatta
     1861 gggtcaaaac taaaagcccc attgtagtta aggtcgatca atgatagtat gtttccatca
     1921 cttatcttct ggccatgctc atcaacaaaa acaggattta ttgttatact tttgccaatt
     1981 cgatatatct gatcgcaatt gtaaactccg aaatctccaa cacttaagcc acgcactata
     2041 tcaggatatt ggtgaccggc gtcaacgcca cggatcatct gagagcttgt tttgggatcc
     2101 catagtatga tatcttcttc ctctattttc tcgccattaa acctgcgtaa cctgaaaccg
     2161 tagtcactat gcttgcgtgt agaatccaat aataccaatg aatcaccttg taatccaaat
     2221 actaaggtat ctacattcat ttcggcgata ttttcgacca actcccacgt atcattattc
     2281 tgcaagccat aaaatgcaat atcatgctgt tgtgttgcaa gcgcatagtc gacattaatt
     2341 tctgtattgt cgcgaatggt aatatctttt ccattcgatg taccgtataa cgaaaacata
     2401 cccgcactgt tgaaacgcat ttcttcgccg tcattttggt aagtcatgga tatttcggag
     2461 aaagccatat cggcggcatt tttatattct gtaaaggcga gtttaacatg atctttcaca
     2521 acattaccct gagcatctac aaaagcatta gcaggaatat gtaaaacagt gcccttgtcg
     2581 ccaatcggaa catcatttgc tttggtaggg tcgatagttt tataaatcgg tgaaggcaat
     2641 agtgcttgtg gaacttgaat tgaggtttta tcgtcgatca acatcggttt tattaaattt
     2701 ttttcagggt ttgccaccca attcgcaacg acttccacgc gcctgttggc ctctttgcct
     2761 tccgctgttt gattgtcgcc aagtggcttt gattccccgg aactatatat ttcaatgttg
     2821 cgcgcatcta cacccatgga aattaaatat gctttaacag aggttgctct tttttctcct
     2881 aaagcaagat tatagccgtc agagccatcg gcatctgtat gtccggtaat ggaaatatta
     2941 ggcgcttgct tgaaggcttc caggtcttta ttcagagctg attgcatttc cggcgttaat
     3001 gcatattcgt tgctgccgaa atggaggatt tgggtttgag catatccgga tgccataagg
     3061 caaagcatgg ccgggatcaa acaaattgat cgtaactggg taagcattat aatgtgttta
     3121 gtgttttata gatgcaggtg cggatgttat tccacaaccg atattacatt tttatccggt
     3181 ttgcacaaca tgaaaactcg ataggataag actttatgat agccactatt ttgacatctt
     3241 attactttga ataagagccg acagctgatc attattcgta tgggttttgg tgatttcttt
     3301 cattttaata tcatagtttc ccgacttgct gattttcatg gccttcatat cggcattact
     3361 cacatagaaa atgcgattgg cttgatttga aacgaaaatc atattatcag atcggctgtc
     3421 gagctgaata ttggcgggat tgttataata aaacgcacca ttaaatttca gatccaccac
     3481 ggtgatattg ttaatacgga ggattggttt tccctgatca tcgaggaaat taccgccgat
     3541 atttacccgc gctaccatag gcgccagggc atcgcaatta taataaccga accctgataa
     3601 agtcaaagat gcttttacgc ttgctcgagt tgtggagccg gtaatacgtt cggtttttac
     3661 cgtgctcaaa ttattgttga aggcattggc aaattgcatg gcagtggctg agtctgcttt
     3721 tacttgtttt gtctcctggg ttttattgcc tgttttgctt gacgtattag aagcaatagg
     3781 cttgccatca acaccgatat tattaatcaa ttgccattct cctttttcat ccagggtata
     3841 aaatccgaca tcattttgat tgcccaccat cgcataatcc attgtgacag atgcattggg
     3901 tgccatttct atagccgcac ctgctgctgt tccctcaatt ttaaacatac ctgaacttga
     3961 gaaacgttga ttgtctgttc ctgttttgta atgcatggga atgccactga ataccatttc
     4021 tcctgaagta gtatattcgg aaaatgaaat ggtcacagtg cttgtaacat ttttacctga
     4081 tgcatctaca aacgagttag caggtatatg caaaatagtt cctctggtcc cgatgggaat
     4141 atcatttggt tttgttgggt cgaaggtgat gatgatcggt gttactttaa atctatccgg
     4201 tgttttaaat gtgcttaatt ttttggcgag tgccaattcg gttgtattaa gtacttctat
     4261 atccactctt cgatcaagtt gtttgtcata tgcgctggca ttgttttgtt ttgccatggt
     4321 ttctccattg ccgaaagcaa caatgacatc agaagaaatt cccttggtag tcaaataatt
     4381 cgtgatagct gcagcgcgtg catctgataa tacatcattg tcgtcattaa caccatcggc
     4441 atcagcataa ccaatcacac gcaagccggg agctgctttt agtttagaca tttgttcatc
     4501 aatgttctta atcgatgccg gtgttaatgt tgcatcgtcg acaccgaaat agagtgaaat
     4561 actttgtgcg ttgcaaacag aaatggcaat caaagaaatg cacaagaacg atgtaatgcg
     4621 tgaagtaaac tgcatacaaa atggtttagg agtgaaagaa tgaaatagaa ggatactgaa
     4681 atagaatatg aaaattctta tttcgtatgg gcgctattca tagcagcata atattttttg
     4741 aagtaggctt gcatgagtgc aaggtcttca tattccggcg gcataccgcg atctgtaccg
     4801 tcggcagcat aataaaacat ttcaccatat ttcaggtaga atgttttttt gaactgccac
     4861 acaaagaaat caggactgat aaatgtttgc acggtataaa aatcagatac gatctggtac
     4921 aatgaggtgt cggagaaata ataacgtact tcccatttat taccgcttgc atcaatactt
     4981 ttggcaatac aaagttgaag tttgccatta taccaatagc cttttaatga agtggtttgt
     5041 ggttggatgt ctttgatcag cggatcgcct gcagcaagca caacactgtc tgtgggcttt
     5101 gaggctttga tcacttcaac tttcttgtcg gcctctttag cccgaatttc ctgcggagtg
     5161 ggttgagcag aaagattccg gccgacaagt gtaatgacgc agagaagcat taatcgtgtg
     5221 tagcattgct tcatatggtc tataacgaaa aacatcgatg tattatgata ggagcttcaa
     5281 aaataggcac aaaaaaaacg caacccatga taggttgcgt tttttttgtg tgaagtgtgc
     5341 ttgcgcttat ttcacaaatt tgttggtaac catattaccg ttagatttca taacagcaat
     5401 gtaaacacct gaaagggcgt cagtaccatt tgcaagttta ccatcccagt tatttacata
     5461 gaaaccattg ctcatatgac cattcacgat agtagcaatg tgctgaccgg catggttata
     5521 aatttcgata gcaacatctg attcagtatc tacagtataa ccgaaagaga aagtgttgtc
     5581 ggcattacgt gtaacgctga attgagcttc tgtaaaagct gagctgaaaa gttcaccgcc
     5641 ggggatagag cgaagatcag caacaggagc attagcaatt gtaagatctg cagcagcagc
     5701 acctacgata gctgtttcat taccaagacg cattgggcaa gtacctaatg accaagtagt
     5761 gctacctgaa ccacttccga gtgcaccgcc aaagcagtag ctagtccagt tgaagtaaat
     5821 aacgccacca ccgtaagcat aagatgtata cgtgaagttg tctacgtaga aagtacatcc
     5881 atcaggtgca gggttagcaa aattcaatgt ccaaacatca gacattttgt cgtaagaacc
     5941 ggttacgatc caatcagatc ctgtgagtac atcagcagtt cctgaaagat cccaatgtcc
     6001 cgggccggtt ttagttgctg ttacgcttgt agtgtatcca aaagcgtcag taaaacaaaa
     6061 accggtgatt tgtgctgtca gggattgtac actgaacaag ccggccacaa gggcaaggag
     6121 taagacgtgt tttttcatga taacatgttt ttggtgatta aattgatttc ataaagctag
     6181 aaaatgcatg tcacaaattc aaattagtcg acattaattt ttgtatgatt tgtaatatca
     6241 gaaacactaa atttatcaat tgtcatatct gcaaataatt aaattgagta gttctatagc
     6301 ataacttgcc atgtaaattc acttcaggcc aaatgttaaa ttattgaaaa tcgcaaatgc
     6361 aaaaaaaagt gaattatttt aaaattcacg gttttccgtg ccttaattaa tcacttttct
     6421 tgttccttat aaagcaaatc ggcaaagcaa gtagcctgat cgagtaattc tgcggatgta
     6481 aaagatactt ggtaaggtga atcaggtgga attaatgtaa tatcggatga aatgaatgca
     6541 tcatgatcga accaataacg gtacttaccc gcaagttcat accatgtagg tccggaaaat
     6601 cctgctattc tgaagcaagt aatttcaaca tgaataagtt tgttgtcttt gaaatagtat
     6661 gtagttgtcc attgagtacc ggattcttct tcaattttaa tcatcgcttt ttcaagaaca
     6721 ttaaaatgaa aatgtccaaa aatatttggc ccgttaaaat catagaaatt ttgaatagaa
     6781 tcccaaggtg caatgcggat gccattgttc cctttgcgat cattaatttt ttcaacttgt
     6841 ttatctatat ctgctattac gtttgtatag aaaaatacct gaccgttgag ccggggcgtc
     6901 atcaacaata gcaaggtaat aaaaatacca tgcagaagat atttaaattg atttgccggc
     6961 atttgtcgat cggtgtgtga aaatatataa ggtaaggtta tatagaaaaa gataaatcgg
     7021 aaaccaggca tatagccatg cgtgtccgaa gcaaacactg atagtaaacg cccaggaaac
     7081 ggcacctagt gtccacgcaa tggttttttg tgatttcgga ttgcggtatc ctgcaataag
     7141 tgttggaatc cccgcaataa aatcggcaag tatggcaaac agtgtagtga tgattgcgtt
     7201 atgtgagctg atgtaaataa tcaaacataa aagcccgagc accagatatt taatatcgct
     7261 gcgctgatat aaaaacgtat tgcgcaaaag agcaatggta aatatgcata aacaacctgc
     7321 ggtagataat gccaagcccg taaggctcga ttgccagcca tcctcgagaa tttgtgccag
     7381 caaccctgtt cccatgatta aaccccatcc cagccaggtg agcagtgagg gattaatctt
     7441 ctttcgccat acatcgcgaa tcagaataag ctgtgccgat gtgatgagca atcctgcaac
     7501 agttgcaatg atggtttcta ttgatttggg aaagagtggt tgcatcttaa gatgccaagt
     7561 tcggtaaata atcttattca gaagtagaat ataacatatt caatatgcga aacatgctta
     7621 tttaggtaaa tattccttca gcaccttttg caaaaggata tcaccatttt ctggattcca
     7681 atcatctgca atagcgatgt caggaatcac tccctttccg ctatagggca catctttatt
     7741 taccacaatc cgataattga tatgtgataa ttcaattttt gtatttggca ttatcagcgg
     7801 tcggtgtacg ccaaaactgc ctgttaattg cgatgaactg cctcctgttt cctcgcccat
     7861 cacaacggcg cgaccataac gttgcatttg tgtggtgaaa atactgctgt tggaaaaagt
     7921 gccgccattg gtaagtatga ttacatttcc gccgtaattg tgcttatagg gcttgactga
     7981 tttgaatcgg ccggtattga cagatttgta tatccgttcg ttggaagaag ttctatgaac
     8041 ccgttcaatt ttatcaatat aattaaaggt gctatccatc aaccatcgca ttacataaac
     8101 accatacatc ataaatccac cttgattatt gcgcaaatca atgattaaag ttccaatgga
     8161 atcgtcgatt gctgtttgca tatatgtgtt aatgctatct ttaaaatgaa taccatatac
     8221 ttttcgcaga attgttttat cccatgtaaa caatgaaagc actgctgcat ccaaagaatc
     8281 gtatcgcgta aacgacaaac ccttgtcacg cgccagtgtc atcggatagt cggggtatcg
     8341 tttttttctt cgctctacta atgtgtcttt tgtgacagcg ggtatttcta caagcacgca
     8401 atcttcaaac aatttatcat ctattctaca tgtagtgctt aactgaaacg tttcgggatg
     8461 accgtagata taactataat attcgtaaaa atatttttcg aggatccaat ttggatattg
     8521 taaattattt ccttc
//
LOCUS       DAOQHY010000133         8479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_4259_length_8479_cov_5.269824, whole genome shotgun sequence.
ACCESSION   DAOQHY010000133 DAOQHY010000000
VERSION     DAOQHY010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8479
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4259_length_8479_cov_5.269824"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1894
                     /locus_tag="PLL28_10775"
     CDS             <1..1894
                     /locus_tag="PLL28_10775"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNF69851.1"
                     /translation="SFNKAYSTPAFNITTTLNTYAKSKYKFEYFFTINDLLKEAERIQ
                     EDVSIDVENLYGGEISIKYRDKNRQKTQEVVNGIADEIVKMSLHRKAESAKTVIGFIK
                     DQIDSIEIQLFNQETLLKNFKQNNQLISPEIAETNIVEKLNEIDKAKFEVMLEEKSLG
                     WLRDYVNNKNNDLEAIGNYFGDMQYNDFSPYINSLITLQREKEDLGLSVQSNDPRIIS
                     INKQIEQVKVNFKSAIDNGQKKLDVRKNYLAQEQDKYESMFYALPEKQGEYARLTRLN
                     DLKEKYYILLLEKQSEYEITLAGMTSDYIILDSAEKGSLVAPVKVKVWGLALLICLGI
                     SFAHVYVRFLTHSVIIGVPDVENATNVPMLGVLPQLAKQKTELPKVVVSSMPKSHIAE
                     AFRSLRSNMQYFLKSDKKSHLITITSTVSGEGKTFVALNLTNVISATGKKVILVDFDL
                     RKPKVHKAFNWRNDKGTSTILIKKHQPEDCIQFNEEIGCDILIAGPVPPNPAELLISP
                     AANDLISYLKEKYDYVIIDNPPVGIVSDSIPILGLADLAIYIIRADYSKRNFVNNVNR
                     LYNDNKLKNLSIIVNDADASGTEYGYGYGYGYGYGYSGYNDYYTDNIRHKSFFRRFFS
                     SKRKRK"
     gene            complement(1951..4779)
                     /gene="uvrA"
                     /locus_tag="PLL28_10780"
     CDS             complement(1951..4779)
                     /gene="uvrA"
                     /locus_tag="PLL28_10780"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNF69852.1"
                     /translation="MAMLDVQGARVHNLQNIDISIPRNSLVVITGVSGSGKSSLAFDT
                     IYAEGQRRYMESFSSYARQFFGGIERPDVDNITGLSPVISIEQKTTNKNPRSTVGTVT
                     EIYDFMRLLYARASDAYSSVTGKKMVRFTEEQIIQSIIKKFDGKRIVLLAPVVRGRKG
                     HYRELFEQIRKQGYLKVRVDGEVKDLLPKMQVDRYKVHDIEIVIDRFKVDEDDMVRIK
                     QSVAQALKAGKQFIMVMEVDGKGIFNYSKKLMCLDSGISYEEPSPNTFSFNSPYGACP
                     KCKGLGVVYEVNVDTLIPDKSKTINNGGILALGEYRDNTLFKQIKSLSGKYKFTLSTP
                     IKDIPKKGLNAILFGSEEDADVDVDDSVFLGENDRWYTLEYGGLAGLIKRCFSNSISE
                     NMRRWAEDFMEKATCDACNGTRLKEEALYFKVNNRNISELAQMDIKDLQLWFTDVEKG
                     MTEKNQIIAKDILKEIRARIGFIKNVGLDYLSLDRPTRTLSGGESQRIRLATQIGSQL
                     VGITYILDEPSIGLHQRDNNKLIAALKELKEGGNTVLVVEHDKDMMLAADHIIDLGPG
                     AGIHGGKLVAAGNPEQFMRAHTVTADYLSGVRSIPVPKTRRKGNGKHITLHGATGHNL
                     KNLTVKFPLGKLICVTGVSGSGKSSLINGTLFPLMQQHYYNSLKKPLPYKKIEGLDHI
                     DKVIEIDQSPIGRTPRSNPVTYIDVFSEIRNLFAIVPEAKIRGYKAGRFSFNVKGGRC
                     EVCEGGGMKVIEMNFLPDVYVECEKCRGRRYNRETLEIYFKGKNIADVLDLTVDHAVD
                     FFEHHPRIYKKIKTLQEVGLGYIKLGQASTTLSGGEAQRVKLAEELSRKDTGNTFYIL
                     DEPTTGLHFEDIRVLMQVITDLVDKGNTVLIIEHNLDVVKLADHIIDLGPEGGAGGGQ
                     ILVEGVPEEVIKHKSSHTGKFLRSELGL"
     gene            complement(4922..5341)
                     /locus_tag="PLL28_10785"
     CDS             complement(4922..5341)
                     /locus_tag="PLL28_10785"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNF69853.1"
                     /translation="MSDPIHTNKAPEPVGLYPHARRVGNLLFLSGIGPRIPGSNAIPG
                     LEQDKNGNFTAFDFEAQCRSVFDNVRIVLEASGATWNDLVDVTVFLTDMQRDFATYNR
                     IYAEYFKDAQPCRTTVGITSLPTPIAIELKCIAAIRE"
     gene            complement(5399..>5600)
                     /locus_tag="PLL28_10790"
     CDS             complement(5399..>5600)
                     /locus_tag="PLL28_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69854.1"
                     /translation="FAGTDVNPLSLSIELSQEIKIAEQARSSVNKLLIWFGLIVKREC
                     PDCAKEVGCTGLGNRNINKTNK"
     assembly_gap    5601..5700
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5701..6231
                     /locus_tag="PLL28_10795"
     CDS             <5701..6231
                     /locus_tag="PLL28_10795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69855.1"
                     /translation="INNVQTTGGGDFPESVNDGIARTIKEMNWTPGKRRVILLLGDAP
                     SLEGSLSEYSTADIISLCKEQDVVMNFYPVVIGMRNEIKGVSKISSTVAKPADVISSV
                     SPNPATNMALVKSNTVDDYTVEIFDINGKILDQKKFHDNNCPIYTDTYPTGVYIVRLT
                     NMTEKTSETVKLVVKR"
     gene            complement(6412..7515)
                     /locus_tag="PLL28_10800"
     CDS             complement(6412..7515)
                     /locus_tag="PLL28_10800"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNF69856.1"
                     /translation="MVLLNRNINPTTGLLAGLTALVMSSCGTYTSAIRTEQPAYTQEQ
                     PQAEETAFIEDFWTDSARTLIAPRLENPVLAETGLRSVEHNSAAIQLPMSEELAQLGE
                     NARLQDEALGDNIGGIDFANPEEAEHFEMYDELFLANELNAGEVFDPSKLTDSIPVYL
                     VSPWSNCYTFPLRQSKVNSDFGWRHSRFHSGIDLDLEVGDDVYASFDGIVKKSDYVSG
                     YGNLVVVKHFNGLETYYAHLSKILVAEGDTVNAGDKIALGGNTGRSTGPHLHYEIRYR
                     GAAINPKYLVDFKNEVLVSESFYLKREHFKPTTESSSSATSTTGKKYVTVKSGDTLSK
                     IAQRNHTTVSKICKLNNISAKKILRPGQRLRVR"
     gene            complement(7528..>8479)
                     /locus_tag="PLL28_10805"
     CDS             complement(7528..>8479)
                     /locus_tag="PLL28_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNF69857.1"
                     /translation="AAGNCVVAKPSEVTPVTAHMLAEICMEVGLPAGVLNIVHGAGPQ
                     CGASIVKHPEIKGVSFTGSTRAGREIASVAAPMFKKLSLELGGKNPVIIFADSDYEEM
                     LNTTVRSSFTNQGQICLCGSRIYVERPLYNRFRSDFVSRVQQLKVGDPAAADTNIGAV
                     VSETHYNKILSYIALAKEEGGTILCGGEAVKMEGEQAGGWYIAPTVIDGLSPQCRTNQ
                     EEIFGPVVSIQPFDTEEEAVTLANNTDYGLAAVIWTNHLSRAHRLGDRLASGIVWINC
                     WLERDLRTPFGGVKNSGVGREGGWEALRFFTEAKNICVKI"
BASE COUNT         2445 a         1960 c         1590 g         2384 t
ORIGIN      
        1 ctcatttaat aaagcatata gcacacctgc gtttaatatc acaacaacgc tcaatactta
       61 tgccaaatcg aaatacaagt ttgaatattt cttcactatt aacgatcttc taaaggaagc
      121 tgaacgcata caagaagacg tttccattga tgttgaaaat ttgtatggcg gcgaaatcag
      181 cattaaatac cgcgacaaaa acagacaaaa aactcaggaa gttgtaaatg gcatcgcaga
      241 tgagattgtg aaaatgagtc tccatagaaa agcagaaagc gctaaaacag taattgggtt
      301 tattaaggat cagatcgact ccattgagat tcaattgttt aatcaggaaa cactcctgaa
      361 aaatttcaag caaaacaatc agcttatcag ccccgaaatt gctgaaacaa atattgtaga
      421 gaagctcaat gaaattgaca aagccaagtt cgaggtcatg ctggaagaaa aaagccttgg
      481 ctggctgcgc gattatgtga ataataaaaa taatgatctg gaagctatcg gcaactattt
      541 tggcgatatg caatacaatg atttttcgcc ttacataaac tcactcatta cacttcaaag
      601 agaaaaagaa gatcttggtc tttctgttca atcaaacgat ccgcgcatta tctccattaa
      661 caagcagata gaacaggtta aggtcaattt taaatcggca attgataacg gacaaaagaa
      721 actcgacgtt cgtaaaaatt acctggcaca agaacaagat aaatacgaat ctatgtttta
      781 tgccttacct gaaaagcaag gagaatatgc caggttgacc aggctgaacg acctaaagga
      841 aaaatattat atcctgctgc tggaaaaaca aagtgaatat gaaatcacat tggccggtat
      901 gacaagtgat tacattattc tggatagcgc agaaaaagga tcattagtag cacctgtgaa
      961 agtaaaagta tggggtttgg cactattgat ttgcctcggt ataagttttg ctcatgtata
     1021 tgtcaggttc cttacacata gtgtaattat cggtgtgcct gatgttgaaa atgccaccaa
     1081 tgtgccaatg ctgggtgtgc ttccgcagtt agcaaaacaa aaaactgagc tgcccaaagt
     1141 tgttgtatca tccatgccga aatcgcatat tgccgaagcc ttccgatcgc ttcgcagtaa
     1201 tatgcaatat ttccttaagt cagataaaaa atcgcatctg attaccatta cgtctactgt
     1261 cagtggtgaa gggaaaactt tcgttgcact gaacttgacc aatgtaatca gcgccaccgg
     1321 taaaaaggta attctggtcg atttcgattt gcgcaagcca aaagtgcata aagcatttaa
     1381 ttggaggaat gacaaaggca caagtaccat tctcataaaa aaacaccaac ccgaagactg
     1441 tattcaattc aatgaagaaa tcggatgcga tatcctcatt gcaggtcccg tgccgccaaa
     1501 tcctgcagaa ttactcatat cacctgctgc aaatgacctc atcagttatt taaaagaaaa
     1561 atacgactac gtaatcatcg ataacccacc ggtaggtatt gtttccgact caattccaat
     1621 tttaggcctt gccgatttag ctatttacat tatccgcgct gattattcaa aacgcaattt
     1681 cgtaaataat gtgaatcgcc tgtataacga caataaactg aaaaacctca gtatcattgt
     1741 taatgatgcg gatgccagcg gaacagaata tggctatggt tatggctacg gatatggtta
     1801 tggatactca ggatataacg actactacac cgataatatc aggcataagt cattctttag
     1861 aagattcttt tcttctaaga ggaagaggaa atgacttatt ctgaattaat atcaatgtgc
     1921 aaagaactac cttcttcgca cttaatcaaa tcacaaaccc aattctgatc gcaggaattt
     1981 tcctgtatgg ctacttttgt gcttgatcac ttcttcagga acaccttcaa ctaatatttg
     2041 cccaccgccg gcgccacctt caggaccaag atcaattata tgatcggcta attttaccac
     2101 atcgagattg tgctcaataa tcaatactgt atttccctta tctacaaggt cagtgatcac
     2161 ctgcatcaac acacgaatat cttcaaagtg taatcccgtt gtaggttcat ctaaaatgta
     2221 gaaagtattt ccggtgtctt tgcgcgataa ctcttccgcc agcttcacac gctgcgcttc
     2281 tccaccgctt aatgtagtag atgcttgccc gagtttaata tatccaagac caacctcctg
     2341 aagcgtctta atcttcttgt aaatacgcgg atgatgttcg aaaaaatcta ccgcgtgatc
     2401 aacggtcaaa tccaatacat cggcaatatt ttttcctttg aagtaaatct ccaaggtctc
     2461 tcgattatac cttcttcctc ggcacttttc acattccaca tacacatcag gaaggaaatt
     2521 catttcgata accttcattc ccccaccttc acaaacttca catcttccac ccttcacgtt
     2581 gaatgagaat ctaccggcct tataacctct gatttttgct tcaggaacta tagcaaacag
     2641 attacgaatc tccgaaaaca catcaatata agttaccgga ttcgaacgcg gtgttctgcc
     2701 aattggactt tgatcaattt caattacctt atctatatga tcaagtcctt ctatcttttt
     2761 atatggaagt ggcttcttta acgaattata ataatgctgc tgcatcaatg gaaataatgt
     2821 gccattgatc aaactggatt ttccgcttcc gctgacacca gttacacaaa ttaatttccc
     2881 caacggaaat ttcactgtca ggttcttcaa attatgacct gtagctccgt gcaaagtaat
     2941 atgtttcccg ttacccttcc tgcgtgtttt aggaaccggg atactcctta caccactcaa
     3001 ataatcagcc gtaacagtgt gcgcacgcat gaactgttcc ggattacccg cagctaccaa
     3061 cttgccacca tgaataccgg cacccggacc gagatcgata atatgatctg ctgccaacat
     3121 catatcctta tcatgttcca caacaagcac agtattaccg ccctctttca attccttgag
     3181 tgctgcaatc aatttattat tatcacgctg atgcaaacca atcgacggct cgtctaaaat
     3241 gtaggtgata cctaccaatt gcgaaccgat ctgcgttgcc aaacgaatac gttgcgattc
     3301 accgccacta agtgttctcg ttggcctgtc gagactcaaa taatcaagcc cgacattttt
     3361 tataaaacct attcgagcgc gaatttcttt caatatgtct ttcgcaatga tctgattctt
     3421 ttcagtcatg cctttttcaa catccgtaaa ccacaattgg aggtctttaa tatccatttg
     3481 cgctaattca gagatgttgc gattattcac cttaaaatac aaggcttctt ctttcaaacg
     3541 ggtaccatta caagcgtcgc aagtagcttt ctccataaaa tcctcagccc agcgacgcat
     3601 gttttcggaa atcgaatttg aaaaacaacg cttaatcaat cccgctaaac caccatattc
     3661 caatgtatac cagcgatcat tctccccaag gaaaacgcta tcatcaacat ctacgtcagc
     3721 atcttcttca cttccgaata aaatggcatt caaacccttt ttaggaatat cttttattgg
     3781 tgtgctgagc gtgaatttat atttacccga taaagattta atttgtttaa ataaggtatt
     3841 gtcgcgatat tctcctaatg ccagaatacc accattgtta attgttttag acttatcagg
     3901 aataagcgta tctacattca cttcataaac aacacccagc cctttgcatt tcgggcatgc
     3961 tccgtaaggt gagttgaaag aaaatgtatt tggcgatggt tcttcataac tgataccgct
     4021 atcaagacac atcaattttt tagaataatt aaaaattcct ttaccgtcaa cttccatcac
     4081 catgatgaat tgctttccgg ccttgagcgc ctgggcaacg ctttgcttga tgcgcaccat
     4141 atcgtcttca tcaaccttaa atctatcaat cacaatctcg atatcgtgca ccttgtacct
     4201 gtcgacttgc attttcggga gcaggtcttt cacttctccg tcaactcgta cttttaaata
     4261 tccttgcttg cgaatttgct caaataattc gcgataatga ccttttcttc cacgcactac
     4321 aggagccaac aacacaatgc gcttgccatc aaatttcttg ataatgcttt gtataatttg
     4381 ctcctccgta aatcgcacca ttttttttcc tgtcaccgaa gaataagcat ccgaagcgcg
     4441 cgcatacaac aagcgcatga aatcatatat ctctgtaaca gttccgacag ttgaacgcgg
     4501 atttttattc gtcgtttttt gttcgataga aattaccggc gacaatccgg taatattatc
     4561 tacatcaggt cgctctatcc caccaaaaaa ttgtctggca taggatgaga aactttccat
     4621 atatctgcgc tggccttcgg cataaatggt atcaaatgcc aatgagcttt ttccacttcc
     4681 gctcaccccg gtgatcacca ccaagctgtt ccgcggaatg gaaatatcga tattctgcaa
     4741 attgtgcacg cgggctccct gcacatcgag catagccatt tcactttcac tgatagccgg
     4801 aagcgaaata gatgtctgag ctatattttt tgttgatttt gccacgcgtc agtaacacga
     4861 aattcgtgca aaggtaaggg atttgaaagc gaaaaaacag caattaccta tgtttcgttc
     4921 actactcccg aatagcggct atgcatttca attcaatggc aatgggtgta ggcagcgatg
     4981 tgattcccac tgttgtgcgg catggctgtg catccttgaa gtactcggca taaatgcggt
     5041 tgtaggtggc aaaatcgcgt tgcatatcgg tgaggaatac tgttacatcc accaggtcgt
     5101 tccaggtagc tcctgaagcc tctaatacta tgcgcacatt gtcgaatacc gaacggcact
     5161 gcgcctcaaa atcgaaggcg gtgaaattgc cgtttttatc ctgttccaag cccggaatag
     5221 catttgaacc cggaatacgt ggtcctattc cactcaaaaa caacaaattc ccaactcttc
     5281 tagcatgtgg atataagcct accggttcag gggctttatt ggtgtggatc ggatctgaca
     5341 ttgcctgcaa tttaaggccg aaattacggt ttatacttgt cgaaagtaac aaaatactct
     5401 acttattcgt tttattgata ttccgattgc ctaaaccggt acaaccaacc tctttcgcgc
     5461 aatccggaca ttcgcgtttc actattaaac caaaccatat gagaagtttg tttacactgc
     5521 ttcttgcttg ctctgcgatt tttatttcct gcgacaactc aattgacaaa gacaaaggat
     5581 ttacatccgt tcctgcaaaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 atcaataatg tgcaaactac cggtggtggc gatttccctg aaagcgtaaa tgacggcatc
     5761 gcccgcacca tcaaagaaat gaactggacg cccggcaaac gcagggtaat tctcttactc
     5821 ggcgatgcac ctagcttgga aggttcgctt agcgaatata gcactgcgga tatcatttca
     5881 ctttgtaaag aacaggatgt agtgatgaat ttttatcccg ttgtcattgg tatgagaaat
     5941 gaaattaaag gtgtatctaa aatttcaagc accgtagcta aacctgccga tgtcatcagc
     6001 tctgtcagcc cgaatcccgc tactaacatg gcgcttgtta aatcaaatac tgtcgatgat
     6061 tatacggtag aaatatttga catcaacgga aaaattctcg atcaaaagaa attccatgat
     6121 aataattgcc cgatttatac cgacacttat cctacaggag tatatattgt tcgcctgaca
     6181 aatatgacag aaaaaacttc tgaaacggtg aaattggtgg ttaaaagata atcctgttaa
     6241 aatcagaaaa cagaaaccat attgatctgc ttttttcgcg ctaaggggca aaggggcgaa
     6301 ggcgcgaaga tggtatgtgt agcatacacg atgtgtatta gggtgctata attaaacaat
     6361 ttgaatagca taaaaaaagc cggcaataac tgtcggcatt tttatgaaag attatctaac
     6421 ccgcaatcgt tgtccgggtc gcaatatttt tttggcggaa atattattca gcttacatat
     6481 cttacttaca gtagtatgat ttcgctgtgc aattttagaa agtgtatcgc ccgacttcac
     6541 ggtaacatat tttttacctg tagttgaagt tgcgcttgat gacgactcag tggtaggttt
     6601 aaaatgctcg cgtttcaaat aaaacgactc agaaacaagc acttcatttt taaaatctac
     6661 cagatattta ggattgatgg ctgctccgcg ataacgaatt tcataatgta agtgcggacc
     6721 ggtgcttcga cctgtattac cacccaatgc aattttatcg ccggcgttta cggtatcgcc
     6781 ttcggcaaca agaattttcg acaagtgagc gtaatatgtt tcgagtccgt tgaaatgttt
     6841 caccacaact aggttaccat aacctgatac ataatcagat tttttcacaa tgccgtcaaa
     6901 tgaagcataa acatcatcac caacttccag atccaagtcg atgcctgagt ggaatcggct
     6961 atgacgccat ccgaaatcgg aattcacctt actttgacgc agcgggaaag tatagcaatt
     7021 actccatgga ctaaccaaat aaacaggaat gctatcggtc agtttcgacg ggtcgaaaac
     7081 ttcaccggca ttcagttcat tcgctagaaa taattcatca tacatttcaa aatgttcggc
     7141 ttcttccgga ttggcgaagt cgataccacc gatattatct cccaatgcct catcttgcag
     7201 acgtgcattt tctccgagtt gtgccagttc ttcgctcatc ggtaattgaa tggcagcgct
     7261 gttatgttca acgctgcgca gtccggtttc tgcgagcacg ggattctcca atcgcggagc
     7321 tatcaaagtg cgtgcggaat ccgtccagaa atcttcgatg aacgccgttt cctcagcttg
     7381 cggttgctcc tgagtataag cgggctgttc agtccggatg gcagatgtgt atgtcccaca
     7441 tgaactcatc accaacgcgg tgagtcctgc gagtaaacct gtggttgggt tgatgtttcg
     7501 atttagcaat accactgtcc tccaatttta gattttaaca caaatatttt ttgcttccgt
     7561 aaagaagcgt aaagcctccc atcccccttc tcttccaacg ccgctatttt taacgccgcc
     7621 aaaaggtgtc cgcaggtcgc gctccagcca gcaattgatc cagacaatgc cggatgccaa
     7681 tcgatcgcca aggcggtgtg cccgcgagag gtgattagtc cagataacag ctgccaaacc
     7741 gtaatccgta ttattggcca aagttaccgc ctcctcctcg gtatcgaaag gttgaatact
     7801 cacaaccgga ccgaagatct cctcctgatt tgtcctgcat tgtggactca gcccatctat
     7861 tactgtcggg gcaatatacc agcctcctgc ctgctcacct tccattttca ccgcttcgcc
     7921 accacatagg atggttccgc cctcttcctt cgctaaggcg atataggaca gtattttatt
     7981 gtaatgtgtt tccgaaacaa cggccccaat attggtatcc gctgctgcgg ggtcgccaac
     8041 tttgagctgc tgaacgcggc ttacgaaatc agaacggaaa cggttgtaga ggggacgttc
     8101 cacataaatt ctacttccgc agagacaaat ttgcccctga ttggtgaagc tgctgcgcac
     8161 tgttgtgttc agcatctctt cgtaatcgct gtctgcaaag ataataaccg gattcttacc
     8221 ccccaactct aaactcagct ttttgaacat cggggcggca acggaggcta tctctctacc
     8281 cgcccgggtg ctgccggtga agctaactcc cttaatttca gggtgtttca ctatcgatgc
     8341 cccacattgc ggacccgcgc cgtgaacaat atttaacacc cctgccggta aaccgacttc
     8401 catacatatc tctgccagca tatgggccgt tacaggtgtc acctctgaag gctttgccac
     8461 cacgcaattg cctgcagcc
//
LOCUS       DAOQHY010000134         8265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_4416_length_8265_cov_5.565652, whole genome shotgun sequence.
ACCESSION   DAOQHY010000134 DAOQHY010000000
VERSION     DAOQHY010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8265
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4416_length_8265_cov_5.565652"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PLL28_10810"
     CDS             complement(<1..479)
                     /locus_tag="PLL28_10810"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HNF69858.1"
                     /translation="MLVIRNLQSLPDLTGCVVTIGTFDGVHHGHCAIIDRITEEAKKR
                     NGKSVIITFYPHPRQIITPGSPVYHLNTLEEKLSKLQARGVDVVVVVPFSREFSDISA
                     EDYVRTFLIEKFHPAVIVFGYDHKFGKNRTGDIHLLKSIAGGYAIHVEEIPAHIIDS"
     gene            complement(504..1226)
                     /gene="truB"
                     /locus_tag="PLL28_10815"
     CDS             complement(504..1226)
                     /gene="truB"
                     /locus_tag="PLL28_10815"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNF69859.1"
                     /translation="MLFTPEEIAEGKFLLLNKPLQWTSHDVIARLRGPLKTFTGNKKL
                     KVGHAGTLDPLASGLLIVLTGKATKRADETQNLDKEYTGIITLGGVTASYDLETPVES
                     ITVTEHITDLLVQQAAQHFTGAFMQLPPAHSSIKIQGKPAYTYAREGEEMKMRPRPIE
                     IFQFEIIAFRNPHIDFRVHCSKGTYIRSLAHDVGQFLGCGGYLSALCRTKIGNYHLEN
                     AYTLDTVIDFLRQSNQHLHQSQ"
     gene            complement(1229..2065)
                     /locus_tag="PLL28_10820"
     CDS             complement(1229..2065)
                     /locus_tag="PLL28_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNF69860.1"
                     /translation="MTIFEAVVLAIVEGITEFLPVSSTGHMIIASSLMGIEADDFTKA
                     FTISVQFGAILSVLALYWKRFLRSVNFYVKLFVAFIPAVVVGVLLGDWIDSLLENVVA
                     VAIALILGGIVLVFIDRLFSADKADDITDPENIPYKNAFAIGVFQCIAVIPGVSRSAA
                     TIIGGLTQKLTRKAAAEFSFFLAIPTMAGAMFKKMVMDEIESGANKGLHPYELLLNNH
                     DNMTAFIIGNVIAFIIAILAMRSFVGYLTKHGFKIFGYYRIIVGAIILILIAAGVDMQ
                     IL"
     gene            complement(2071..2349)
                     /locus_tag="PLL28_10825"
     CDS             complement(2071..2349)
                     /locus_tag="PLL28_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HNF69861.1"
                     /translation="MDNSTSPKKETPKTTSPAGPMMVLGKQNYYLILFGLAVIIIGFI
                     LMSGGKSPDPNVFNADEVYSTRRIVVAPIVVILGFAIEIYAVFHKSKK"
     gene            complement(2369..3250)
                     /locus_tag="PLL28_10830"
     CDS             complement(2369..3250)
                     /locus_tag="PLL28_10830"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNF69862.1"
                     /translation="MSQQAGKSLQRKQTHYVNAILITALALVMLGVMSILMISFKYEE
                     ANIKEDLVISAYLKDGLTQTDVDKLIKRITAEPSVHDTHYISKEAAMDIFKEKFNEDP
                     GEILEDVNPLPASIDISLDAAAVNVDSINAFRERLQLYPEITLTRANEALISSVDTDL
                     KVAGFVIAGLSILFLIIAIAIIDKTIRLSMYSNRFLVRSMQLVGATRGFITGPYVRRS
                     IINGVISAFIAAFILMLGVIYVHNNYNYWDFSNPTIKRGFMFTMLMLLAVGVLITRFS
                     TRSSVHKYIKMKLDELY"
     gene            3370..>6008
                     /gene="leuS"
                     /locus_tag="PLL28_10835"
     CDS             3370..>6008
                     /gene="leuS"
                     /locus_tag="PLL28_10835"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNF69863.1"
                     /translation="MDLTIGLISEIEKKWQSEWRKSDLYKVTEDPSRPKYYVLDMFPY
                     PSGSGLHVGHPLGYVASDIISRYKRLKGFNVLHPMGFDAFGLPAEQYAIETGQHPAVT
                     TEKNIAYFREQLDRMGFCYDWNRGVKTSDPSYYKWTQWIFMQLFKHWYDPFLNKAQPI
                     ADLEQMLEKEGSARLIEKDICDTLITADQWKSMSGDEKQKFLMNFRLAFLDHAAIWWC
                     EALGTVLANDEVKDGVSERGGHPVEKIRMRQWFLRITAYAERLLSGLETVDFSDSMKE
                     MQRNWIGKSEGALVDFKVEGSENQIRIFTTRPDTIFGATFMVLAPEHELVQQITSIDQ
                     QDAVSQYINYVKSRSERDRMSEVKKVTGQFTGAYAIHPFTGNKIPIYIAEYVLAGYGT
                     GAIMAVPSNDDRDAAFAEKFNLPIIPVVDQSNFPGAAREDKVGVMINSDMLNGLPVTE
                     AISTMINAIATKGIGRRKINFRLRDAGFSRQRYWGEPFPVYYINDIPYLLDESELPVI
                     LPQVESYKPAGAAQSPLSGVTDWVNLPNGARRETDTMPGYAGSSWYYFRYMDVHNDKA
                     FASKEALDYWQDIDLYIGGTEHAVGHLLYSRMWCKFLFDIGLVPKEEPYKRLVNQGMI
                     QGWSKIVYRIVGTNTFVSKQLKDQHETTELRVDIKMVAGDILDTEAFKKWRTDFANAE
                     FILEDGKYYCGTELEKMSKSKHNVVNPDDVINQYGADCFRLYEMFLGPLDQGKPWDTK
                     GITGVQGFLRKLWRLCVDEQGNMRVTNDEPSNEEFTILHRTIKKVTDDIERMSMNTVV
                     STFMICVNDLSAGKCYKRAIIEPLLVMTAPFAPFLSEELYNKLGNDVSVHKASWPEVD
                     ERYLVRSSIPYAVQVNGKLRATV"
     assembly_gap    6009..6108
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6109..6299
                     /locus_tag="PLL28_10840"
     CDS             <6109..6299
                     /locus_tag="PLL28_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69864.1"
                     /translation="SSIPYAVQVNGKLRATVDIPTDMSDADIQATVLALDQVVKHMEG
                     KSPKKVIVVKGKIVNVVV"
     gene            6313..6852
                     /locus_tag="PLL28_10845"
     CDS             6313..6852
                     /locus_tag="PLL28_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69865.1"
                     /translation="MKKSLLLVIICVCSSLASFAQEDDQLSDNQSNAYAQAQIMVGAF
                     EAGDYNRLLDYTYPAILVMGGGRDMLLSIISQMMSDMKAQGVVVDSARVGLPSQVYPA
                     GDELHALIPQYVWMSTSDTYIYSESTLLAVSDDNGSRWYFLDTRQLTPELKDSFFPDY
                     NSFLVIPPVKEVVVTPKVK"
     gene            6859..7734
                     /locus_tag="PLL28_10850"
     CDS             6859..7734
                     /locus_tag="PLL28_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HNF69866.1"
                     /translation="MTEISQHIKLVECPRDAMQGLHDMIATDKKVEYINQLLRVGFDT
                     IDFGSFVSPKAIPQMADTREVLAQLDLSETKSKLLAIVGNVRGAEDAAIFENIHYLGF
                     PFSISETFQQRNVNSSIAESVERVKEIKAICDGNNKQLMIYISMGFGNPYGDPWSADV
                     AASWVEKLVAMGIRHVSLADTIGVANPESIQYLFGHLIPAFPDVEFGAHLHTTPDAWL
                     EKIDAAYKSGCRRFDGAIKGFGGCPMAADDLTGNMATENIIQYFNHIGAELNINRSEF
                     LESVRKAMQTFPVDA"
BASE COUNT         2427 a         1595 c         1826 g         2317 t
ORIGIN      
        1 aaagaatcga tgatatgtgc cggtatctct tcaacgtgta tggcataacc tccggcaata
       61 gattttaata aatggatatc gcctgtcctg tttttgccga atttatgatc atatccgaaa
      121 acgattacgg caggatggaa tttttcaatc aaaaaagtac gcacataatc ttctgcgctg
      181 atgtcagaaa attctctcga aaaaggaacc acaaccacaa catcaactcc tcgcgcttgt
      241 aacttactta atttttcctc cagcgtattg agatgataca ccggtgatcc cggagtaata
      301 atttgacgtg gatgcggata aaaagtaatg ataacacttt tgccattcct ttttttagct
      361 tcttcggtaa tgcggtctat aattgcgcaa tgcccgtgat gcacgccatc aaaggtgcct
      421 atggtaacca cacatcctgt tagatcgggc agcgattgta agtttctgat cacaagcatg
      481 gcagcaaagg taattattgt atgttattgc gactgatgaa gatgttgatt tgattgtcga
      541 aggaaatcaa taaccgtatc taaagtatag gcattttcga gatgataatt cccgattttt
      601 gttctgcaaa gggctgataa atatccgcca catcctaaaa attgtccaac atcatgcgcc
      661 aaagaccgaa tgtaagtccc tttgctgcaa tgcacccgaa agtcgatatg cggattgcga
      721 aatgcgataa tttcaaattg gaatatttca atcggacgcg ggcgcatttt catttcttcg
      781 ccttcacggg cataagtata ggcaggttta ccttgaattt taatggaaga atgcgcaggg
      841 gggagttgca tgaaagctcc ggtgaaatgt tgagcagcct gttgcaccaa taaatcagta
      901 atatgttcag taacggttat tgattcaaca ggagtctcca gatcataact tgcagtaaca
      961 cctcctaagg tgatgatgcc ggtatattcc ttatccagat tttgcgtttc atcggcgcgc
     1021 ttagttgcct tgcctgtcag cacaataagt aatccggatg caagtggatc aagggtgcct
     1081 gcgtgaccaa cttttagttt tttattaccg gtaaatgttt tcaaagggcc gcgcagtctt
     1141 gcaataacat cgtgactcgt ccattgcaag ggtttattca ataaaagaaa ttttccttcg
     1201 gctatttctt ccggtgtgaa caacatgatc acaaaatttg catatcaacg cctgcggcaa
     1261 ttaaaatgag gataattgcg cctacaatta ttcgataata accgaaaatt ttgaatccat
     1321 gtttagtaag atatcccaca aaactcctca tggcaagtat tgctataata aatgcgataa
     1381 catttccaat aataaatgca gtcatattat cgtggttgtt gagtagcaat tcatatggat
     1441 gcaatccttt atttgcaccg ctttcaattt catccatcac cattttttta aacatagctc
     1501 ctgccattgt gggaatagca aggaaaaacg aaaactctgc agcggctttg cgtgtcaatt
     1561 tttgcgtgag tccgccaata attgttgctg ccgatctgga tacaccggga ataacggcta
     1621 tgcattgaaa tacacctata gcaaatgcat ttttataagg aatgttttca ggatcggtaa
     1681 tgtcatcggc tttgtcggca ctaaataatc tatctataaa taccaataca attccaccaa
     1741 ggataagtgc aatggctacg gctacaacat tctccagcaa actatctatc caatcgccca
     1801 acagcacacc aactacaaca gccgggataa aggcgacaaa taatttaaca tagaaattca
     1861 cggatcgcaa aaaacgtttc caatacaaag caagtaccga gagtattgct ccaaattgaa
     1921 ctgaaatggt aaatgccttc gtaaaatcat cagcttcgat gcccattaat gaggaagcaa
     1981 tgatcatatg acccgtagat gaaacgggaa gaaattcggt gatgccttca acaatggcga
     2041 ggaccaccgc ttcgaaaatg gtcatgctta ttatttttta gacttatgaa atacagcgta
     2101 gatctcgata gcaaaaccga gaataactac gataggcgcc acaacaatgc gacgggtgct
     2161 gtatacttca tcggcattga acacatttgg atcagggctt tttccgccac tcattaaaat
     2221 aaatccaata atgattactg caagaccgaa caggatcaaa taataattct gtttcccaag
     2281 caccatcatt ggaccggcgg gcgatgtggt tttaggagtt tctttttttg gcgatgtcga
     2341 gttgtccata tcctgaagtg ttggatgatt aataaagttc gtcgagtttc attttaatat
     2401 atttatgcac tgaacttctg gtgctaaacc gggtaatcag cacacctaca gcaagtaaca
     2461 tcaacatggt aaacataaag ccacgtttga tggtgggatt gctgaagtcc caataattat
     2521 aattattgtg cacgtaaatt acaccgagca ttaaaataaa tgcggcgata aaagcagata
     2581 tcacaccatt aataatgctt cggcgaacat aaggtccggt tataaatcca cgcgttgcgc
     2641 caaccaattg catactgcgc accaaaaaac gattcgaata catgcttaaa cgtattgttt
     2701 tatcgataat ggcaatagca atgatgagga ataaaattga taagcctgca atcacaaatc
     2761 cggcaacttt caaatcggta tctacacttg aaatcaatgc ctcgtttgca cgtgttaatg
     2821 taatttccgg atataattgc aagcgttcgc gaaatgcatt tatagaatca acatttacgg
     2881 cagcagcatc cagactgata tcaattgatg ccggtaatgg atttacatct tcaagaattt
     2941 ctccgggatc ttcattgaat ttttctttga agatatccat cgccgcttct ttggaaatat
     3001 aatgcgtgtc atgcaccgaa ggttccgcag tgatgcgctt tatcaatttg tcgacatcgg
     3061 tttgtgtgag accatccttt aaataagccg aaataacgag gtcttctttg atatttgctt
     3121 cttcgtattt gaagctgatc atcagaatgc tcattacacc caacatcact aatgccagtg
     3181 ccgtaatcag tatggcgttg acatagtggg tttgcttacg ttgcagcgat ttcccggcct
     3241 gttgactcat ctgctgcaaa aatacgtctt tgacctcatt ccggcctttg ttcatctaca
     3301 cttgcatgtg ggtaactgca atccatagcc aataaccctc gaatagcccg aaaacactaa
     3361 ctttgaggca tggatttaac gattggactg atcagtgaga tcgagaagaa atggcaatcg
     3421 gagtggcgaa aaagtgattt atacaaggtt accgaagacc cttcgcgccc gaaatattat
     3481 gtgttggata tgttcccgta tcccagcgga agtggcttgc acgttggtca tcccctcggt
     3541 tatgtagcca gtgatattat ctccagatat aagcgcttaa agggtttcaa cgtattgcac
     3601 cctatgggat tcgatgcctt cggacttccc gccgagcagt atgccattga aacaggtcag
     3661 catccggcag ttactaccga gaaaaatatt gcctatttcc gcgagcagct cgatcgcatg
     3721 ggattttgtt acgattggaa caggggagtg aaaacttctg acccttccta ttacaaatgg
     3781 acccaatgga tctttatgca gttgttcaag cattggtacg atccattcct gaataaagcg
     3841 caaccaattg cagatcttga acagatgttg gaaaaagagg gtagtgcgcg actgattgaa
     3901 aaggacattt gtgatacact cataactgcc gaccaatgga aatctatgtc gggtgatgag
     3961 aagcagaagt ttctgatgaa tttccgcctt gcatttttag atcatgccgc catttggtgg
     4021 tgcgaagcat tgggaacagt gttggcaaac gatgaagtga aagatggtgt aagtgaacgt
     4081 ggtggtcatc cggttgagaa aatccgcatg cgccaatggt tcctgaggat aactgcctat
     4141 gcagaacgct tattgtcggg attggaaaca gtagacttca gcgatagcat gaaagaaatg
     4201 caacgcaact ggatcggtaa aagcgaagga gctttagtag attttaaggt tgaaggaagt
     4261 gaaaatcaaa tccgcatttt taccactcgt cccgatacca ttttcggtgc aacatttatg
     4321 gtgttggcac ctgaacatga acttgtgcag cagattactt ccatcgatca gcaagatgct
     4381 gtatctcagt atatcaatta tgtgaaaagt cgcagtgaaa gagatcgcat gtcagaagta
     4441 aaaaaagtga cgggacaatt caccggtgct tatgcgattc atccgtttac cggaaataaa
     4501 attccgattt atattgccga atatgtgctt gcaggatatg gaaccggcgc tatcatggca
     4561 gtgcctagca atgatgatag agatgctgca tttgcagaga aatttaattt gccgataatt
     4621 ccggtagtcg accaaagcaa ttttccgggc gcagcgcgcg aagataaagt tggtgtgatg
     4681 atcaattcag atatgctgaa cggattgccg gttactgaag ctatttcgac aatgattaat
     4741 gccatcgcaa caaaaggtat tggcagaaga aaaattaatt tccgcttgcg cgatgcaggg
     4801 ttttctcgtc agcgatattg gggtgaacca tttccggttt attatatcaa cgatatccct
     4861 tatttgctcg atgaaagtga gttgcctgta atattgccac aagtagaaag ttataaacct
     4921 gccggtgcgg cacagtcgcc attaagtggt gttaccgatt gggtgaattt acccaacggt
     4981 gcaagacgag aaacggatac catgcccggt tatgccggat caagctggta ttatttcaga
     5041 tatatggatg tgcataatga taaagcgttt gcatcgaaag aagcgttaga ctattggcag
     5101 gatattgatc tgtatatcgg tggaactgaa cacgctgttg gccacttatt gtattcacgc
     5161 atgtggtgca aatttttatt cgatatcgga ttagtgccaa aggaagaacc atataaaaga
     5221 ttggtgaatc aagggatgat tcaaggttgg tcgaaaattg tttatcgcat cgttggcacc
     5281 aatacttttg taagcaaaca attaaaggat cagcacgaaa caaccgaatt gcgtgtcgat
     5341 ataaaaatgg ttgctggcga tattctggac accgaagcat ttaaaaaatg gcgcactgat
     5401 tttgccaatg cagaattcat cttggaagac ggtaaatatt attgtggaac tgaattggaa
     5461 aaaatgtcca aatcaaaaca caatgttgta aatcctgatg atgtcatcaa tcaatatggt
     5521 gcagattgtt tccgtttgta tgagatgttt ttaggtccgc tggatcaggg aaaaccatgg
     5581 gatacaaaag gtattaccgg tgtgcaaggg ttccttcgca aactatggcg tttatgtgtg
     5641 gatgagcagg gaaatatgcg cgtaactaac gatgagcctt caaatgaaga gtttacaatt
     5701 ttacatcgta caataaaaaa agtgaccgac gacattgagc gcatgagcat gaatactgtt
     5761 gtcagcactt ttatgatttg cgtgaatgat ttaagtgccg gtaaatgtta taaacgtgcc
     5821 ataattgaac ctttgcttgt aatgacggcg ccatttgctc cattcttgtc ggaagaatta
     5881 tacaataaat tgggaaatga tgtttcagtc cacaaggctt catggccgga ggttgatgag
     5941 cgttatttgg tgagaagcag cattccttat gccgtgcaag tgaacggaaa attgcgcgca
     6001 acggttgann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga agcagcattc
     6121 cttatgccgt gcaagtgaac ggaaaattgc gcgcaacggt tgatatcccg acagatatgt
     6181 ctgacgctga tattcaagct accgttttgg cattggatca ggtggtaaaa catatggaag
     6241 ggaagtcacc gaaaaaagta attgttgtaa aaggcaagat tgttaatgta gttgtttaaa
     6301 aaattaatgg gcatgaaaaa atcactattg cttgtaatta tttgtgtttg cagcagtctg
     6361 gcaagctttg cgcaggagga tgatcaatta tccgacaacc aaagtaatgc gtatgctcag
     6421 gcacagataa tggttggtgc atttgaagca ggtgattata atagattgct agattatact
     6481 tatcctgcca ttttagttat gggtggagga agagatatgt tgttgtctat catatcacag
     6541 atgatgagcg atatgaaagc gcaaggtgtg gtggtggatt ctgcccgagt tggattgcca
     6601 agccaggttt atcctgcagg cgacgaattg catgcgttga ttccgcaata tgtatggatg
     6661 tcgacttccg atacttatat ttacagtgaa agcacattgc ttgcagtatc agatgataat
     6721 ggaagcaggt ggtattttct cgacaccagg caattaactc cggaattaaa ggacagtttt
     6781 tttcctgatt ataatagttt tcttgtcatc cctccggtaa aagaagttgt tgtaacacca
     6841 aaagttaaat aaaggaaagt gaccgaaatt tctcaacata ttaaacttgt agaatgtcct
     6901 cgcgatgcga tgcaaggatt gcatgatatg atagcaaccg ataaaaaggt tgaatacatc
     6961 aatcaattgc ttcgcgttgg atttgatacc attgatttcg gcagtttcgt ttcgccaaag
     7021 gcaattcctc aaatggctga tacgcgtgag gttttggcac aacttgatct tagcgaaacc
     7081 aaaagtaaat tgctcgccat agtaggaaat gtgcgcggtg cagaagatgc agcaattttc
     7141 gaaaatattc actacctcgg ttttccattc tctatttcag aaacatttca gcaacgcaat
     7201 gtcaattcga gcatagcaga aagtgtggag cgcgtaaaag aaatcaaagc aatttgcgat
     7261 ggtaacaaca agcaactcat gatatatata tcaatgggtt tcggcaatcc atatggcgat
     7321 ccatggagtg ccgatgtagc agcttcgtgg gtagagaaat tggtggcaat gggcatacgc
     7381 catgtttcac tggcagatac cataggtgtc gctaatcccg agagtattca atacttattc
     7441 ggacatctga tacctgcttt tccggatgtg gaatttggcg ctcatttgca tactacaccg
     7501 gatgcgtggc tcgaaaaaat tgatgctgct tacaaaagcg gatgtcgtcg tttcgatggc
     7561 gccataaagg gttttggcgg ttgcccgatg gcagctgatg accttacagg gaacatggcc
     7621 acggaaaata ttatacaata cttcaaccat atcggcgcag aactcaatat caatagaagc
     7681 gaattccttg agagcgtccg taaagcaatg cagaccttcc cggttgatgc ttgatgtttg
     7741 aaatcagact tgtctcccca atggcgggta tcaggtatca ggtaaaaatt cacgagtcac
     7801 gaatcacgag tcacgattgg tagtgagtag tgggtagcga gtagtgagac aaatgcggtg
     7861 gaatgtgctt tgcattacct ctgtgcaact ctgtgttcat ctccgtggcc tctgtggtta
     7921 aatggtgagt gggtgagtgg gtcagtgggt gagtttgcat tacctctgtg caactctgtg
     7981 ttcatctccg tggcctcttt ggttacatgg gtttttgagg attgttttag tatccggaat
     8041 tggtatcaag tatcaggaaa aaattcacga ttcacgattc acgagtcacg attggtagtg
     8101 agtagtgggt agtgagtagt gagaaaattc acgagtcacg actcacgact cacgattcac
     8161 cactcaccca ctcacccact gacccactca cccattcacc cattttttcc ttaaatttac
     8221 cttaccaaat tgtaacattt acatgaagaa aatactcctc cttgt
//
LOCUS       DAOQHY010000135         8242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_4428_length_8242_cov_5.791743, whole genome shotgun sequence.
ACCESSION   DAOQHY010000135 DAOQHY010000000
VERSION     DAOQHY010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8242
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4428_length_8242_cov_5.791743"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..377
                     /locus_tag="PLL28_10855"
     CDS             156..377
                     /locus_tag="PLL28_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69867.1"
                     /translation="MLPAITAAMKEEKCYEDCRGSQNDDITPKLAKAIVCVINAKANA
                     YQIDGIPNAVTQSFESIVPYPAKTDEQEK"
     gene            complement(423..2717)
                     /locus_tag="PLL28_10860"
     CDS             complement(423..2717)
                     /locus_tag="PLL28_10860"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNF69868.1"
                     /translation="MKHIHAAILMLLIPSIAFSQKYVELYNAGKYEKAIDQAEKAIDK
                     NSKDLNAYLTKAMCNLHLYLDPVTQPAHQAGVLSAVNTLELIVKKDKDESFQQQHQPE
                     IDSILKAGGDMATKYFEKGDVSRADKLLADLITLQPRPEYYFLAGRIAQQADNDDLAI
                     HYYNTASAKIYKDSKAGKPVSEDFIPMFVALANAIGNQGDLKSAFVVYDRAFELFPSE
                     SLSDSCYYFLSALAEKHAYANDNIRIDQLIQYLDTIPAPVAKDSRFYALKWDCIFTRY
                     YNMQGYYIAANIREANSYLTQWACKDQFTAAADTLLSAMLRNTYLKMNVYEIAVSRDP
                     DMLQDYLTMKSCLNNGNMQQAEASYFDKIVYALEIKDFTFAGKLIYNLSSIKAYATQS
                     KAFEKQLFTGLQQLSFDELSQIDLYALSLMFPGNTVGKKLQKDESIRQITALIDKGDF
                     TNAGIKVRAQMRLDPKDMTVRTLYKTWVIKDYVTTYYNSATYSDLSVWNGNISKCDPG
                     KLPDSIHNKVLMRLNYYRRLVGIPDNCVFDKELNKKCQAAALMMTANHDLSHGPPPDW
                     SCYSPEGATGAGNSNLSLGYGGAEALSGQVEDDGGNNGAVGHRRWILNPFRKVYGHGS
                     TDDAMALWALGGNDADQPKEIREQYMDTYVAWPPEWYSPAPLLPARWSLGMYHADFSE
                     AKVQMFQGNNEIGLEVLPLEYGYGLSTIVWEPGINMYGAKGDQTYKVVVSHIGIDSTW
                     DEATQSYKKEYVDHTYYVIAVQMQ"
     gene            2781..3155
                     /locus_tag="PLL28_10865"
     CDS             2781..3155
                     /locus_tag="PLL28_10865"
                     /inference="COORDINATES: protein motif:HMM:NF017935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HNF69869.1"
                     /translation="MASTKSKFSVVLQNKCPRCQEGEMFVDPNPYHLNNMAKMHRNCA
                     KCGLRFEPETGFYYGAMYLSYALGVFSSLIIFAILNFVFRIPTLWAFIIVALSWIGLS
                     PMLFRFSRSLWLSLFTNRHRDI"
     gene            complement(3164..3814)
                     /locus_tag="PLL28_10870"
     CDS             complement(3164..3814)
                     /locus_tag="PLL28_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69870.1"
                     /translation="MRNISLLLICFIGFTACKNYTGIPDSFDYGRMDGNVYSNTYFDF
                     TFSMPDGWQVMENSVMDSLRRKTQEDLAEKDPELAKTVKAADVRTANLLTIICSKDPN
                     YFLYSANLNFVAENVKIAINVKDGKDYLESAKKNLGNLEYSLDFKGDIYKMDINGVEF
                     YVMNLINHYNGLDIKQSYYASIINGFAFTFVASWITDEQRTFIDEAISKMKFIKEA"
     gene            complement(3893..5365)
                     /gene="dacB"
                     /locus_tag="PLL28_10875"
     CDS             complement(3893..5365)
                     /gene="dacB"
                     /locus_tag="PLL28_10875"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HNF69871.1"
                     /translation="MKKIISFFFILVLSCGVFAQTKIDSIGLNKLKGGLKAMSVDADL
                     VHANWGFCLMDPTTGKVLAEYGSEKSLTPASSQKVITTIAAMNILGADYTYKTYVEYT
                     GTIGKDSVLTGDLYIRGTGDPTLGSMRIDSLVTYDSLLEVWALKIKEKGIATINGKII
                     ADASYFEDYTTVGSWNWDDIGQYYGAGASGLNFAENKYTVYFSSNASRAKIDSIRPEI
                     DGMTVWNDVRVGGTGDEAYIYGAPDNYYRYVTGTIPANRKAYEVDGSMPDPPLFLALA
                     LKSALDTIGIHVTGQATTQYAVVRAGLADKSERKALFTHSSVPLKQMIFETNQHSINL
                     YAETFLKTIGKKQKNDGSREAGVNAENAFYSTAGLKLDAMHIEDGSGLSRLNYFSPKD
                     MCSILRFEMQQNSFTDFLPTLPVAGQSGTLKTMGDNTAAEGRIFAKSGSMYKVRSYSG
                     YVQTKSGQKLVFCVMVNNYTCGSAEIRAKLEKLMVLMAGV"
     gene            complement(5395..6114)
                     /gene="dapB"
                     /locus_tag="PLL28_10880"
     CDS             complement(5395..6114)
                     /gene="dapB"
                     /locus_tag="PLL28_10880"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNF69872.1"
                     /translation="MKVALIGHGKMGKAVEQVMHRFPNAHIAAIFSSSNGHRLSVDAL
                     KGIDVAIEFTTPLTAVAHIKLCIDAGVPVVTGTTGWYQSLPEITDYCKQKNGALLYAA
                     NFSIGVHLFLAANARLAALMQHQHQYTLNISETHHTQKLDAPSGTAIRIADTISEHAP
                     RYKGWVLEPEQKEHAIPITAHRIDHVPGIHTVEWKSSVDTITLTHSAQSREGFAEGAW
                     LAAQWIIGKQGVFSMQDVLGI"
     gene            complement(6111..6734)
                     /locus_tag="PLL28_10885"
     CDS             complement(6111..6734)
                     /locus_tag="PLL28_10885"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HNF69873.1"
                     /translation="MRHLLITLLCVFAASQMHAQQDSIMTQDMVDTTLAQTDSLHTPF
                     YKNLWSHDPHSVGRAALYSAVIPGLGQAYNGKYWKIPIVYGALGTSGYFIYYWYDFFD
                     ELRTAYIYRTDEDPLTIDTQYDYIPSDELLLQYVSQSKRYLDVMVIVTAAIYTLNIID
                     AVVDAHLYDFNVSDDLSMQVQPHYFFGYNQINPLQGTMGISLQLSLK"
     gene            complement(6738..7631)
                     /locus_tag="PLL28_10890"
     CDS             complement(6738..7631)
                     /locus_tag="PLL28_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNF69874.1"
                     /translation="MSQKKPDLGKGIRALLQSMEDEKTDPIVTQKKTQELSGTSNIAI
                     GNIELNPFQPRSDFDETALKELSESIKVHGVIQPITVRTVGTNKFQLISGERRLRASK
                     LAGLTDIPAYVRNANDQEMLEIALIENIQREDLNAIEIAIHFRRLLDECNLTHEALAD
                     RLGKDRSSVSNYLRLLKLPPDIQRGLKEKLISMGHARALISLEDPIAQLAVYKEAIAG
                     ALSVRDVEALVRNYQPKKQKSAKSQTKDTSFAVRKLQDELSSHLATRVIIKPGSAGRG
                     EIVISYFSDDDLYRLKEIIEG"
     gene            complement(7705..>8242)
                     /locus_tag="PLL28_10895"
     CDS             complement(7705..>8242)
                     /locus_tag="PLL28_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNF69875.1"
                     /translation="GAEIELINHPNRERILKSVLDEVKNNYDFIFIDCSPSLGLITVN
                     ALTSADSVLVPVQCEYFALEGLGKLLNTIKIVQNRLNNPLQIEGILLTMYDARLRLSN
                     QIVTEVKRHFADLVFDVIIHRNTKLAEAPGFGKPVVMYDAECTGAINYLNLAREILQK
                     NSATPSQLGNELLNIHQN"
BASE COUNT         2336 a         1800 c         1617 g         2489 t
ORIGIN      
        1 cctgcctcgc ctgcttagca tacggcttag caaggcgggt attcagttga gctacgaggc
       61 caaatggagt gcaaaggtac taaatggatt gaatatttca aaatatttcg cacaagtatt
      121 tataaatcaa caagttacgt ttaggctgga aatttatgtt acctgcgata acggcggcga
      181 tgaaggagga aaagtgctac gaggattgcc gcggaagcca aaacgacgat attaccccaa
      241 agctggccaa agcgattgta tgtgttataa atgccaaggc aaatgcatat caaatagacg
      301 gtatcccaaa tgcggtaacg cagtcctttg aaagcatcgt accctacccc gccaaaaccg
      361 atgagcaaga aaagtaatat ggcaagtgta gaggtcagca gttgcagttt taaaaaataa
      421 aattactgca tttgcactgc tatcacgtaa taggtatgat cgacatattc ttttttgtag
      481 gactgtgtgg cttcatccca agtggaatcg ataccaatat gactaacaac cactttgtac
      541 gtttggtctc ccttagcgcc atacatattg attcccggct cccaaacaat ggttgacaat
      601 ccatagccat attcaagcgg tagcacctcg agtcctattt cgttattgcc ttgaaacatt
      661 tgcactttag cttccgaaaa gtcggcatga tacattccca gcgaccagcg ggcaggaagc
      721 aatggagcgg gactatacca ttcaggaggc caggcaacat aggtatccat atactgttca
      781 cgaatctcct tcggctggtc tgcatcattc ccaccaagcg cccataaggc catggcatca
      841 tcggtagatc catgtccata aacttttcta aatggattta gaatccatct gcggtgacca
      901 acagccccat tattacctcc atcatcttcc acttgtcccg ataacgcctc agcaccaccg
      961 tatcctaatg ataaatttga atttccggca ccggtagcac cttccggtga atagcaactc
     1021 caatcgggag gtggtccatg cgacaagtca tgatttgcgg tcatcattaa tgcggcagcc
     1081 tgacattttt tatttaattc tttatcaaaa acacaattat ccggaatccc tacaagcctg
     1141 cgataataat tcaatcgcat caacacttta ttatgaatag aatccggcag cttaccaggg
     1201 tcgcactttg aaatattgcc attccatacc gacaggtcac tataggttgc actattataa
     1261 tatgtggtga cataatcttt gatcacccag gttttatata aggttctcac ggtcatgtct
     1321 ttgggatcca atctcatttg agcacgtacc tttatgcctg catttgtgaa atcacccttg
     1381 tcgatgagtg ctgtgatctg acgaatagat tcatccttct gcaatttctt gccaacggta
     1441 ttacccggaa acatcaggct aagggcatac agatcgattt gactaagttc atcaaatgat
     1501 aattgctgca aaccggtgaa taattgtttt tcaaaagctt tcgactgtgt ggcataggct
     1561 tttattgatg acaggttata aatgagtttt ccggcaaacg taaaatcttt tatctctaat
     1621 gcgtaaacaa ttttatcgaa ataagaggcc tcagcttgtt gcatatttcc attattcagg
     1681 caggatttca tggttaaata atcttgcaac atatccggat cgcgggaaac agcaatctca
     1741 tatacattca ttttcagata tgtatttctt aacatcgcag acaaaagggt atcagcggca
     1801 gcggtgaatt gatctttaca cgcccattgg gtgagatagc tgttcgcttc tctgatattg
     1861 gcagctatat aatatccttg catgttgtaa taccttgtga aaatacagtc ccattttaat
     1921 gcataaaatc tcgaatcctt tgcaacggga gcgggaatgg tatcaagata ttggatgagt
     1981 tgatctattc tgatattgtc attagcataa gcatgttttt cagccaaagc gctcaggaaa
     2041 taatagcatg aatctgaaag cgattcggaa ggaaataatt caaaggcacg atcatacact
     2101 acgaatgccg atttcaaatc tccctggtta ccgatggcgt tcgctagtgc aacaaacatt
     2161 ggaatgaaat cttcactaac aggtttgccc gcctttgaat ctttataaat tttcgccgaa
     2221 gcagtgttat aatagtggat agcgagatca tcgttatctg cctgctgcgc aatgcgaccg
     2281 gccagaaaat agtattccgg tcgtggctgc agggtgataa ggtcagctag cagcttatcc
     2341 gcccgtgata catctccctt ctcaaaatac tttgttgcca tatcgccacc ggctttcaaa
     2401 atgctgtcaa tttcgggttg atgctgctgt tgaaatgatt cgtccttatc ctttttgaca
     2461 atgagttcga gtgtatttac ggctgacaat acgcctgcct gatgtgcggg ctgagttacg
     2521 ggatcaaggt ataaatggag gttgcacatg gcctttgtca gataggcatt gaggtccttg
     2581 ctgtttttgt ctatggcctt ctcagcctga tctatggcct tttcgtactt accggcgttg
     2641 tacaattcaa cgtacttctg gctaaaggca attgacggaa taagcagcat taggatagct
     2701 gcgtgtatgt gcttcatctt cagtgactaa acgcacaaag gtactatcct attttgcact
     2761 taaatagggt aattttgcgc atggcttcaa cgaaatcaaa atttagtgtc gttttacaga
     2821 acaaatgccc ccgttgtcag gagggtgaaa tgtttgtaga cccgaatccc tatcacctca
     2881 acaatatggc gaaaatgcat cgcaactgcg cgaaatgcgg cttgcgcttt gaaccggaaa
     2941 caggatttta ttatggagcg atgtatttga gctatgctct tggtgttttt tccagcttga
     3001 taatattcgc tattctcaac tttgttttca ggataccaac cttatgggcg tttatcattg
     3061 tagccttatc atggatagga ttatcaccca tgcttttcag atttagcaga tcactgtggt
     3121 taagtttatt cactaacagg catagagata tctgattttc atttcaggct tctttaataa
     3181 acttcatttt acttatagcc tcatcaataa aggtgcgctg ttcatctgta atccatgaag
     3241 ctacaaatgt gaaggcaaag ccattaataa ttgaggcata atacgattgt ttgatgtcaa
     3301 gtccgttgta atgattaatc aaattcatta cataaaactc aacaccattg atatccattt
     3361 tgtagatatc tcctttaaaa tccagcgaat attctaaatt gcccaaattc ttctttgcag
     3421 attcaagata atccttgcca tcttttacat taatggcaat ttttacattt tccgcaacaa
     3481 aattcagatt agcactgtat aagaaataat tggggtcttt gctgcaaatg attgtgagca
     3541 gatttgctgt tcgaacgtct gcggctttta ctgtttttgc taattcagga tccttctctg
     3601 ccagatcttc ctgtgttttt ctgcgaagac tgtccatcac agaattctcc attacctgcc
     3661 aaccatcggg catagaaaat gtaaaatcaa aataggtatt ggaatacaca ttaccatcca
     3721 ttcgaccata atcaaatgaa tcaggaattc ctgtataatt tttacaagca gtgaacccaa
     3781 taaaacatat caaaagcaaa ctaatattcc gcatatttca gattttgaat aaatctacga
     3841 caatatagat tggtatgcaa tgaggttaga taaaacttat ggtacaggta aatcagacac
     3901 ctgccatcaa taccatcaac ttctccaatt tagcgcgtat ttctgcagat ccgcaggtgt
     3961 aattattcac cattacacaa aatacaagtt tctgtccgct ttttgtttgc acatatcctg
     4021 agtatgaacg cactttatac atggaaccac ttttggcaaa tatccggcct tctgctgcgg
     4081 tattatcgcc catggtcttt aaagttccgc tctggcccgc aacaggtaag gttggcagaa
     4141 aatcagtaaa tgaattctgc tgcatttcaa aacgcaaaat gctacacatg tcttttggag
     4201 aaaaataatt tagtcgcgac aaaccactgc catcttcaat atgcatggca tcgagtttta
     4261 atccggcggt ggaataaaat gcattttcgg catttacacc tgcttctcta ctgccatcgt
     4321 ttttctgttt cttcccaatt gttttcaaaa atgtttcggc atacaaattg atgctatgct
     4381 gatttgtttc aaatatcatt tgtttcagtg gcacggagct atgagtaaac aatgcttttc
     4441 tttcagactt atctgccagt cctgcgcgca ctacggcata ttgcgtggta gcctgtcccg
     4501 taacatgtat accaattgtg tcaagtgccg attttaatgc cagggcaaga aataatggcg
     4561 gatcgggcat actgccatcc acttcatacg cttttctgtt tgcgggaatt gtgccggtta
     4621 cgtatcgata ataattatca ggagcgccat aaatatatgc ttcatctcct gttcctccga
     4681 cacgcacatc attccacaca gtcatgccat caatttccgg acgaatgctg tcaatttttg
     4741 cacgcgaagc atttgaagag aagtatacag tatatttatt ttcagcgaaa tttaatcccg
     4801 atgcaccggc tccgtaatac tggccgatgt catcccaatt ccaactccct accgttgtat
     4861 aatcttcgaa ataagaagca tcagcaataa tttttccatt gattgtcgca ataccttttt
     4921 ccttaatttt caaagcccat acttccagca aagaatcata agttaccagg gaatcaatgc
     4981 gcatgcttcc caaggtagga tcaccggttc cacgaatata gagatcaccg gtgagcacag
     5041 aatccttacc tatggtaccg gtatattcga catacgtttt gtaagtataa tcggcaccca
     5101 aaatattcat ggccgcaatt gtcgtaataa ctttttggct tgaagcaggt gtaaggcttt
     5161 tttcactgcc atattcagca agtacttttc cggtagtagg atccatcaga caaaatcccc
     5221 agttggcatg cacaagatct gcatctaccg acattgcttt taatccacct ttaagcttat
     5281 tcaacccaat tgaatcaatt tttgtctggg caaaaactcc acaggataaa accaaaatga
     5341 aaaagaatga aataattttc ttcatgcctt attaaacgca attagttaca acaattatat
     5401 ccccaaaaca tcctgcatgc taaaaacacc ttgctttcct attatccatt gtgcagccaa
     5461 ccaagcgcct tcagcaaagc cttctcgtga ttgagctgaa tgggtaagag taatggtatc
     5521 cacactcgat ttccattcaa ccgtatgtat accgggaaca tggtctatac gatgagctgt
     5581 tattgggatt gcatgttctt tttgttcagg ctccaaaacc catcccttat atcgcggagc
     5641 atgctcactg atcgtatcgg caatgcgaat cgccgtgcca ctgggcgcgt ctaatttttg
     5701 tgtgtgatgc gtttctgaaa tattgagcgt atattgatgt tgatgctgca ttaatgccgc
     5761 aagtcgcgca tttgccgcca gaaataaatg cacccctatg ctgaaattcg ctgcatacaa
     5821 taaagcgcca tttttttgct tacaatagtc cgtaatttcc ggtaatgatt gataccaacc
     5881 ggttgtcccc gtaacaacag gaacacccgc atcgatgcat aatttaatat gagcaactgc
     5941 agttaatggc gttgtgaatt ctatggctac atctattccc ttcagtgcat caactgaaag
     6001 cctgtggcca ttcgacgagg aaaatatggc agcgatatgt gcattcggaa atctgtgcat
     6061 tacttgctct acagcctttc ccatttttcc gtgtccaatg agtgctactt tcattttagc
     6121 gacaattgaa gtgaaatacc catagttccc tgcagtggat ttatctgatt ataaccgaag
     6181 aaataatgcg gctgcacttg catcgaaaga tcatcagaaa cgttgaaatc atataaatgc
     6241 gcatcaacta cggcatcaat aatatttaat gtataaatag cagctgtaac gataaccatc
     6301 acatccaggt agcgctttga ttgcgataca tattgcaata ataattcgtc ggatgggata
     6361 taatcatatt gcgtatctat ggtcaatgga tcttcatctg ttcgataaat atatgccgtg
     6421 cgcaattcat caaaaaaatc ataccagtaa tatatgaaat agcccgaggt gccgagagct
     6481 ccataaacaa ttggaatttt ccagtattta ccgttgtatg cttgtcccaa ccccggaata
     6541 actgcactat acaaggcagc gcgaccaaca ctgtgcggat catgcgacca tagatttttg
     6601 taaaatggtg tatgcagtga atctgtttgt gccaatgttg tatcaaccat atcttgcgtc
     6661 attatgctat cttgctgtgc atgcatttgg cttgcagcaa acacacataa cagggttata
     6721 agcaaatgac gcatgaatta tccttcgatg atttctttta gtcgatacag atcatcatca
     6781 gaaaaatagg aaatgacgat ctctcccctt cctgcacttc cgggtttaat gattacacgg
     6841 gttgcaagat gcgacgaaag ctcatcctgc aatttacgta ccgcaaatga agtatccttg
     6901 gtttgcgatt tggcagattt ttgttttttc ggttgataat tgcgcaccaa tgcttccaca
     6961 tcgcgaaccg acaatgctcc ggcaattgct tctttataaa ctgctaactg cgcaatcgga
     7021 tcttcaagtg aaataagggc acgtgcatgt cccatggaaa tgagtttctc ttttaatccg
     7081 cgctgaatat ccggaggtaa ctttaataat cgcaagtagt tacttacaga cgagcgatct
     7141 tttcctaacc tatcagccag tgcttcgtgg gtgagattac attcatcgag cagacgtcgg
     7201 aaatgtatgg caatttcaat ggcgttgagg tcttcgcgtt gaatattctc tatcaaagcg
     7261 atctccagca tttcctgatc attggcgttg cgcacataag cagggatatc tgttaatcct
     7321 gccaatttag atgcacgcaa acgacgctca ccgctaatca attgaaattt attggtgcca
     7381 acggtgcgaa cggtgattgg ctgaattacg ccatgcactt taatactctc gctgagttct
     7441 tttaaggcag tctcatcgaa atcagagcgc ggttgaaatg gattgagttc aatattaccg
     7501 atggcaatat ttgaagtacc cgacaactcc tgtgttttct tctgggttac gattgggtcg
     7561 gtcttctcat cttccatgct ttgcagcagg gcacgaatac ctttaccaag atccggtttt
     7621 ttctggctca tgatttctgt taatgctata aaacgatatt ctcccggaaa ttatttaccg
     7681 ggaagcatac ctgaataaat ataatcaatt ctggtgaata ttcagcaatt cattaccgag
     7741 ctgactaggc gttgcgctgt ttttttgcag gatttcgcga gccaggttga ggtaattgat
     7801 agctcctgtg cattcagcat cgtacattac caccggcttt ccgaatcccg gtgcttccgc
     7861 cagcttggta ttgcgatgga tgatcacatc aaataccaga tcggcaaaat ggcgtttcac
     7921 ctctgttact atctggttgc tgagtcgcaa acgggcatca tacatagtaa gcagtatgcc
     7981 ttcgatttgc aatggattgt tcaatcggtt ttgaacaatt ttaattgtat tcaacaattt
     8041 tccgagacct tcaagcgcaa agtattcgca ctgcacaggc accagcactg aatcggcaga
     8101 tgtaagtgcg ttaactgtga taagtcccaa tgaaggtgaa caatcgataa aaatgaagtc
     8161 gtagttattt ttcacctcat caagtacgct tttcaaaatg cgttcgcgat tcgggtgatt
     8221 aattaattca atttcagcac cc
//
LOCUS       DAOQHY010000136         8208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_4460_length_8208_cov_5.658653, whole genome shotgun sequence.
ACCESSION   DAOQHY010000136 DAOQHY010000000
VERSION     DAOQHY010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8208
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4460_length_8208_cov_5.658653"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="PLL28_10900"
     CDS             complement(<1..812)
                     /locus_tag="PLL28_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69876.1"
                     /translation="MRILLLAKKPPYPARDGEAIAIGQMAKGLADLGHAVTLLYMNTA
                     KHHFPAAEIPPMYGVTFISHEVRAGIGAIGALRNLLGTKPYHVDRFFSKSFKDKVLTL
                     DAEHHFDVIQAEGLYLVQYLPLSGTDSVAKRIYRSHNLEFEIWQNVADHQFKKFRKRY
                     LQIQANRLKSYELEVLKTHVDGVLPISTVDTGYYQTLMPKLACMHAPTGMEIPGASPN
                     LVTGHDIYCIGGLDWLPNIEGLYWFFEAVWPKIKAMLPESTMHLAGRNAPKN"
     gene            875..1621
                     /locus_tag="PLL28_10905"
     CDS             875..1621
                     /locus_tag="PLL28_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNF69877.1"
                     /translation="MLLNKKIVVVLPAYNAAKTLEQTYAEIPFDIVDDVVLVDDASRD
                     ETVEVGKKIGIKHIIRHEKNKGYGGNQKSCYNKALELGADIVVMLHPDYQYTPKLITS
                     MANVIAQDLYPVVLASRILGRGALKGGMPYYKYVFNRFLTFSQNVLMRQKLSEYHTGY
                     RAFSGEVLKNINFMANSDDFVFDNQMLAQIFFAGYEIAEVTCPTKYFDEASSINFKRS
                     VIYGLGVLRTSMQYRMQKWGMANHAYLRKP"
     gene            1740..2519
                     /locus_tag="PLL28_10910"
     CDS             1740..2519
                     /locus_tag="PLL28_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69878.1"
                     /translation="MFRKHTITLIALMMGIFFKIQAQSIVLCESYDDNGTATGIYNSW
                     DIKSGGSYVYILYKQDARFSSGSWYLYIDYDWTNSGTLTAYETLPLEPARDKNWLVYD
                     FKFTDAGNYRAYIMHDGVELASVNFNIGWQEGEAPTTSGSSTIDTYYYEDSELKFCGS
                     VDAEGNPMGIATEFYMGSNGKASFKAYLDNDFKAIQTSLIYVDVYKEGVTDPVDAFSI
                     TVQPDWDYIHFQLDLTSAGLYHVDLYNADDIFINTADVVIR"
     gene            complement(2618..3091)
                     /locus_tag="PLL28_10915"
     CDS             complement(2618..3091)
                     /locus_tag="PLL28_10915"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNF69879.1"
                     /translation="MTSQLSQQIAKQFRDAHDGDNWTAVNLKTLLSDVTRDEAVQQIE
                     GFNSIATLVNHTGYYLDIVIPVLRGAPLVGKDSDSFILPDLSDQEKWQALVDKVFADV
                     EVFAALIESFPDEKWWEPFNDGKYGNYYRNITGIIEHLHYHMGQIAFLKKMLRKS"
     gene            complement(3088..3675)
                     /locus_tag="PLL28_10920"
     CDS             complement(3088..3675)
                     /locus_tag="PLL28_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69880.1"
                     /translation="MKRPELIDLTLKITGIIMVIQLVNSVIQLIFTIPQITFIWQGPD
                     DGQFSYVIALFGIYVLGPLIYAVVIYQLTFKSDKWARRILRPHQMEEEINLKIEPQVM
                     LNVSILIASIFLLFWNIPTLLMAFGQFIASNTPRHMDYMPVDFDKNGLIEPILNIAVA
                     LILFTWSSPISHYLANRIVKATPSEEQEQPKIDDI"
     gene            complement(3760..4524)
                     /locus_tag="PLL28_10925"
     CDS             complement(3760..4524)
                     /locus_tag="PLL28_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69881.1"
                     /translation="MKRTVIAALLIVTIVGCFDHAYFEGPDQFDEDFEAYHSVDDMFV
                     DDDVLWSFTQLTMPENTLIIDSTFSHGGLKSIKCFAQAGAEGTVSKASFSKQNMAFWE
                     GETMRASGWYYIPGDNELNWLFIMDLEEQTAIGAGPGIRLAIVDNCLMVEHKYLEDDI
                     YQPTTSQVPFPRNEWVYLNWEVYLSTKDDGTIKLYQNGELILSNNHTVTLPQDILYFQ
                     QGTKGMYSSIEFGATANPTGADAIVWVDDIFVEVVK"
     assembly_gap    4677..4776
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4777..5122)
                     /locus_tag="PLL28_10930"
     CDS             complement(<4777..5122)
                     /locus_tag="PLL28_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69882.1"
                     /translation="MHTLSAQSEDHAKYISLELAGSGGLGSINYEQDFKDFGKWDLTW
                     RAGFSLAPIDKNNGVGLVFPLMINARFGEGTSKLDMGLGQGVTITTKGAFFMLTTAAV
                     GYRYQKETSPWYF"
     gene            complement(5230..5592)
                     /locus_tag="PLL28_10935"
     CDS             complement(5230..5592)
                     /locus_tag="PLL28_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNF69883.1"
                     /translation="MQGLRTCIYKVTDLEAGKAWYSKAFNQTPYFDHPFYVGFDIGGF
                     ELGLLPEEEEKHVYGNNMLVYWGVDDIQSSYDHLVSVGATSHEAPHAVGDDIMVATVF
                     DPWGNIVGIIYNPGFKMA"
     gene            complement(5997..6890)
                     /locus_tag="PLL28_10940"
     CDS             complement(5997..6890)
                     /locus_tag="PLL28_10940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69884.1"
                     /translation="MRAKKQFTLQQYSCLAGALLLIQNNSFSQAIYTDIEPDVELQFQ
                     GDFVYIDMDSDSNNDFFLLKRSSYFTNTYYEWYRFNHILKCGPNFNPENSIAGIFATN
                     GAGGGTTYFPYAINAGEIINSDLSFQYGHYQLMGIGFFDVDETPWVWHYFIGSWTPSI
                     DSMFLGVRFLGGDDCKHYGWIRCSTADSAKTLIVHDFAYESKCETPIIAGDTIGDTTT
                     VAVSNLLELNAEIFVYNKQVFVSCKVSHQLEIFDSSGKKILDSNRQQGAYNIDMQPFP
                     KGIYIVKLTSGSQMISKTISM"
     gene            complement(6890..>8208)
                     /locus_tag="PLL28_10945"
     CDS             complement(6890..>8208)
                     /locus_tag="PLL28_10945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69885.1"
                     /translation="PNSGIAGGCVTEDCDDWTNDQWQFDITDERLSNAYKAVIGLGSN
                     GFGNFKQFIRTRYAESYDQQRLDLMFYGNEAWKKDLWTTEWNLKDKNTDYSANDPEQY
                     ILSSFCNSFEHGWLIQEWFLKDIKQNYAKNYRKGFHTYSTFHAFGGGSYYTLLLQSDR
                     ADREKHLDELGNPDTLITPPADQNLFLKRTMYHTFDMLSEIKNHNLKYLPSNFTAYVH
                     NPNIQPTVFIDKSSKKLYIYYSNMKTETQSYVLEKGNILMLYPGSTALAYGEALIYNI
                     APTQLYSNSGNSFLYKMNICYNTTDQQHPFEIQYINGPVINEPECSGLPGGAICLTVP
                     ANSYGYIEVPFYITPREGLTLSDDQIEVYPNPTANAFRITCDIPEELLNTFYVSVFDI
                     HGSLLLNTVISQNQDIDISNLPSSIYFIVISNEEKSFSVSKKIIKI"
BASE COUNT         2368 a         1593 c         1650 g         2497 t
ORIGIN      
        1 aaatttttgg gtgcatttct tccggcgaga tgcatagtag attccggaag catggccttt
       61 attttcggcc aaacggcttc aaaaaaccag tataaaccct caatattcgg cagccaatcg
      121 aggccgccaa tgcaataaat atcatgtcct gtaacgaggt ttggactggc accgggaatt
      181 tccatgcctg ttggcgcgtg catacaagcg agtttcggca tgagcgtttg ataatagccg
      241 gtatcaacgg ttgaaatcgg taaaacgcca tcgacatgcg ttttcagcac ttcaagttca
      301 taggatttta agcgattagc ctgtatttgg agataccttt tcctgaattt tttgaattga
      361 tgatcggcca cattctgcca gatctcaaat tcgagattat gtgagcgata aatgcgtttg
      421 gcaacagaat cggttccgga gagcgggaga tattgcacca ggtagagtcc ttcagcctga
      481 ataacatcga aatgatgttc agcatccaga gtaagcacct tatccttaaa gcttttgctg
      541 aaaaagcggt cgacatgata gggtttagta cctaaaaggt tgcgaagagc tccaatagcg
      601 ccaatgcctg ccctcacctc atggctgata aacgtaacgc catacatagg tggtatttca
      661 gctgccggaa aatggtgttt ggcggtattc atatacaata aggtaaccgc atgaccaaga
      721 tcggcaagcc cttttgccat ttggcctata gctatagctt caccatcacg ggccggatag
      781 ggaggttttt ttgcaaggag gaggatgcgc atgagcacgc aaaattacgg ttttacatgg
      841 ccttcggcat caatattgta aatttgccgc agctatgttg ctgaataaaa agatcgtggt
      901 ggtgcttccg gcttacaatg ccgctaaaac cctggagcaa acctatgccg aaattccttt
      961 tgatattgtt gatgatgtag tattggttga cgatgccagt cgcgatgaga cggtggaagt
     1021 ggggaagaaa atcggcatca aacatatcat ccgtcacgaa aagaataaag gttacggcgg
     1081 aaatcagaaa tcttgttaca acaaggcgct ggaattgggt gccgatattg tggtgatgtt
     1141 gcatcccgac tatcagtata caccgaagct gattaccagc atggcaaacg tgattgcgca
     1201 ggacctgtat cctgtagtgc tggcaagtcg catattgggt agaggagcat tgaagggtgg
     1261 gatgccgtat tataaatatg tatttaatcg tttccttacc ttttcgcaga atgtgttgat
     1321 gcgtcaaaag ttgtcggaat accataccgg ataccgtgca ttttcgggcg aagtattgaa
     1381 gaacatcaac tttatggcaa acagcgatga ttttgtgttc gacaatcaga tgcttgccca
     1441 gatatttttt gccggttatg aaattgctga ggttacctgt ccgaccaaat atttcgatga
     1501 agcctcttcc attaatttta aacgttcggt aatttatgga ttaggtgtcc ttcgcacatc
     1561 tatgcaatat aggatgcaga agtggggaat ggcaaatcac gcctacctgc gcaaaccctg
     1621 atcgccattg cgcaaaagcg ataatttaat gatacaccgt tcttttcaaa attatttttc
     1681 ctttctcctt tctcctttct cctttattaa ttaattttac tttgctaaaa aacatcaaaa
     1741 tgttccgcaa acacacaatc accttgatcg ccctgatgat ggggatcttt ttcaagatac
     1801 aggcacaaag tattgtactc tgtgaatctt atgatgataa tggtaccgca accggcattt
     1861 ataattcatg ggacatcaaa tcaggtggtt cctacgttta tatattatac aaacaagacg
     1921 cgagattttc atccggatcc tggtatttat acatcgatta cgactggact aattccggta
     1981 cgttgacagc atatgaaacg cttccgttag aaccggctcg tgataaaaat tggctggtgt
     2041 atgattttaa atttacggat gcaggtaact atcgcgccta tatcatgcac gacggtgttg
     2101 agctggcttc tgtgaatttt aatattggtt ggcaagaagg tgaagcacca acaacttccg
     2161 gttcttcaac catcgacacc tattattatg aagattctga attaaaattc tgcggctcag
     2221 ttgacgctga aggtaatcct atgggaattg ctacggaatt ttatatgggc agtaatggta
     2281 aggcaagttt caaagcctac ctcgataatg attttaaggc tattcaaacc tctttaatat
     2341 atgtcgatgt atataaagaa ggtgtaactg accctgtaga tgcattttca attaccgttc
     2401 aacctgattg ggattatatt cactttcagc tcgatttaac ctccgcaggt ttataccatg
     2461 ttgatttgta taatgctgac gacatcttta tcaatactgc ggatgtggtg ataaggtgag
     2521 gcgagtgggt cagtgggtga gtgggtgagt gagtttgcat gcaggctttg cgacttagta
     2581 actttgcgac tttgcgcgaa acaaacacat accacaatca actttttcgc agcatttttt
     2641 tgaggaaagc aatttgcccc atgtgatagt gcaggtgttc tataataccg gtaatattgc
     2701 gataataatt accgtattta ccatcattaa acggctccca ccatttttca tcagggaatg
     2761 attctatcag tgcggcaaat acttcaacat cagcaaacac cttatcaacc aatgcctgcc
     2821 atttttcttg atcggacaaa tccggcaaaa taaaactatc actgtccttt cccaccaaag
     2881 gtgcaccacg caatacggga atcacaatgt ctaaataata acctgtatga ttcaccaaag
     2941 ttgcaatgga attaaatcct tcaatctgtt gaacagcttc gtcacgggta acatcggata
     3001 acagcgtttt cagatttacg gcagtccagt tatcgccatc atgtgcatct cggaattgtt
     3061 tagcgatttg ttgcgaaagt tgtgatgtca tatgtcgtcg attttaggtt gttcttgttc
     3121 ttctgatgga gttgctttaa ctatgcgatt cgccagataa tggctgatag ggctagacca
     3181 tgtgaaaaga attaatgcaa cagcaatatt gaggattggc tcaatcagcc catttttatc
     3241 aaaatcaaca ggcatgtaat ccatatgtcg aggcgtattg gaggcgataa attgaccaaa
     3301 tgccatgaga agtgtaggga tattccagaa caacaaaaat atggatgcaa tcaatatact
     3361 tacattaagc ataacctgtg gttcaatctt gagatttatt tcttcttcca tttgatgcgg
     3421 tcgtaagatt cgacgagccc atttgtcgga tttaaatgtc aattgataaa taactactgc
     3481 ataaataaga ggccctaaaa cataaatgcc aaaaagagca atgacatatg aaaattgacc
     3541 atcatccgga ccttgccaaa taaatgtaat ctgtgggatg gtaaatatca attgaatgac
     3601 actattcacc aattgaatta ccataataat ccccgttatt ttgagagtaa gatcgatgag
     3661 ttcagggcgt ttcatgagtt tgttttatca aatatgtaaa aaatatgtga cgaaacaaaa
     3721 cccggtattt ttatcggtag gtgattttct gagggataat tatttgacca cctccacaaa
     3781 aatgtcatcc acccaaacaa ttgcatcagc gccggtagga tttgctgtag cgccaaattc
     3841 aatgctgcta tacattcctt tggtgccttg ctgaaaatag aggatgtctt gcggtaaggt
     3901 tacagtatga ttgttgctga gtattagttc accattttga tacagtttaa tggtgccgtc
     3961 gtcttttgta gataaataca cttcccaatt cagatacacc cattcatttc ttggaaatgg
     4021 aacctgtgat gttgttggtt gataaatatc atcctcaaga tatttatgtt caaccatcag
     4081 acaattatct acaatagcaa gtcggatacc gggaccggca ccgatggcag tttgttcttc
     4141 aagatccatg ataaacaacc aattgagttc attatctccc ggtatatagt accatccact
     4201 tgctctcata gtttcacctt cccagaatgc catattctgt ttactgaaac ttgctttcga
     4261 tactgttcct tcggcacctg cctgagcgaa gcatttaata ctttttagtc ctccgtggct
     4321 aaatgtggag tcaattataa gcgtgttctc cggcatggtg agttgtgtga atgaccatag
     4381 tacatcgtca tcaacaaaca tatcgtcgac gctgtgatat gcttcaaaat cttcatcgaa
     4441 ttgatcaggc ccttcgaaat aggcatgatc gaaacatccg acaatggtga caatgagcag
     4501 tgcagcaatt acggttcttt tcattttacc gaggtattta tggtgtagcc aatactcaat
     4561 cctccccaat gttgccactg gaaatcaact aaataagaga ttaatggtgt gtatgatgcc
     4621 ttaaaatacc agggactcgt ttctttttga tatcgatatc cgacagctgc tgttgtnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntaaa ataccaggga ctcgtttctt
     4801 tttgatatcg atatccgaca gctgctgttg tcagcataaa aaatgctcct tttgtggtga
     4861 tggtaacacc ttgtcccaaa cccatatcca gtttagaagt tccctctcca aaacgtgcgt
     4921 ttatcatcag aggaaaaacc agtccgacgc cattattttt atctatcggt gccaggctaa
     4981 aacctgcacg ccatgtgagg tcccattttc cgaaatcttt aaaatcctgc tcgtaattaa
     5041 tggatcccag tccaccactt ccggcgagtt ctagagaaat atattttgcg tggtcttcag
     5101 actgtgcact gagtgtatgc aaacacatta ataagaggca taacgagaaa cagcgaaaca
     5161 actgcatcat gaaaaaacga atttatataa aaatgtagta tacggaatga acgaatgaat
     5221 caattgatgt catgccattt taaacccggg attgtaaata atgccgacaa tatttcccca
     5281 tggatcgaaa acagtagcta ccatgatatc gtcgccaaca gcatgcggtg cttcatgtga
     5341 agttgctccg acagatacaa gatggtcata agatgattga atgtcatcta caccccaata
     5401 taccaacatg ttatttccgt aaacatgttt ttcttcttct tctggcaata gtcccagttc
     5461 aaaacctccg atatcaaatc cgacataaaa tggatggtcg aaatacgggg tttgattgaa
     5521 ggcttttgaa taccaggctt ttccggcttc aagatctgtt actttgtaaa tgcatgtgcg
     5581 cagtccctgc ataatgaatg attattcaac aagtatactg aaattgaaga aaacacctat
     5641 catttttttc taatcggttt tgcagcaacc agcgtatatg cgtcgtggag taattttttt
     5701 agcatgccga tgtcaatttt tgttgtatcg gcgtgcgtcc aaccttggtg accccatttg
     5761 tttggtacgg gttcgatgtg gggagaggaa ttagtgtatg cttcctgttg caattgacta
     5821 agtttaacgg tggaagaggt cgtatttgga tcgtaggtgc aaaaaatttt gccttttatg
     5881 cgaaaggata ttttttcaaa atgcgcctcc tgcgttacat ccggaaatgc acaggcgata
     5941 ttgcaaagtt gatggaaagt ggccatatca ataatccttt tcaaaaaact tccaagtcac
     6001 atagatatcg tcttagatat catttgcgat ccgcttgtca attttactat gtaaatccct
     6061 tttgggaaag gttgcatgtc gatattgtat gcgccttgct gccggttgct atcaagtatt
     6121 ttcttgccgc tgctgtcgaa tatttctaat tgatgggata ctttgcagct tacaaatacc
     6181 tgcttgttgt aaacgaatat ttcagcgttt aattctaata aattactcac agccactgtt
     6241 gttgtgtcgc caatggtatc gccggcaata atgggcgttt cacattttga ttcataagca
     6301 aaatcgtgca caatcaatgt cttggcagaa tcagcggttg agcacctgat ccaaccataa
     6361 tgtttgcaat cgtcacctcc gagaaaacgg acgcctagaa acatggaatc tatactcgga
     6421 gtccatgagc caatgaaata atgccatacc caaggtgttt cgtctacgtc aaaaaaccca
     6481 atccccatca attgataatg tccatactga aaactcaaat cgctattgat tatttctccg
     6541 gcgttaatag cgtaggggaa ataagtagtt ccaccgcctg caccattggt ggcaaaaatt
     6601 ccggctatgc tattttcagg gttaaaattt ggaccgcatt ttaaaatatg gttaaacctg
     6661 taccattcat agtaagtatt tgtgaaataa ctactccttt ttaacaagaa aaaatcatta
     6721 ttgctgtcac tatccatatc aatgtaaacg aaatcgccct gaaattgtaa ttccacgtct
     6781 ggctcaatat cagtatatat agcttgagaa aatgaattat tttgtatcag aagcaatgct
     6841 cctgcaagac aggagtattg ttgtaatgta aattgttttt tagctctcat taaattttga
     6901 tgattttttt tgatactgaa aagctttttt cttcattgct gataacaata aaatagatac
     6961 ttgaaggaag gttgcttata tcaatatctt gattttggga gattactgta tttaatagta
     7021 ggctaccgtg aatatcaaaa accgatacat agaatgtgtt gagtaattct tcgggtatat
     7081 cacatgtaat gcgaaaggca ttcgcagttg gattgggata cacctcaatt tgatcgtccg
     7141 aaagcgtcag cccttctctt ggagtgatat aaaatggcac ttcaatataa ccataggaat
     7201 ttgcagggac tgtaaggcaa atggcacctc ccggtaagcc ggagcattca ggttcattga
     7261 taacagggcc attgatgtat tgtatctcga acggatgctg ttgatcagta gtgttatagc
     7321 agatattcat tttatataaa aaactattcc cactattgga atagagttgt gtaggagcga
     7381 tattataaat caatgcctca ccataggcca aagcggtaga accaggatag agcatcaaga
     7441 tatttccttt ttccaataca tagctttgtg tttcggtttt catattggaa tagtaaatat
     7501 acagtttttt acttgatttg tcaataaaaa ctgttggttg tatattggga ttatgcacat
     7561 aagcggtaaa attagagggc aggtacttaa ggttgtgatt ctttatttcg ctaagcatat
     7621 caaatgtatg gtacatagta cgtttcaaaa acaaattctg atctgcaggt ggtgttatta
     7681 aggtgtccgg attaccaagt tcgtcaagat gtttttctcg atctgctcta tctgattgta
     7741 atagcaaggt ataatatgag ccaccaccaa aggcatggaa ggtggaataa gtgtgaaaac
     7801 cctttctata atttttggca taattctgtt ttatatcttt taaaaaccat tcttgtataa
     7861 gccatccatg ttcaaatgaa ttacagaaag aacttagaat gtattgttcg ggatcattcg
     7921 ctgagtaatc agtattttta tctttaaggt tccactctgt tgtccacaaa tcttttttcc
     7981 aagcctcatt cccataaaac atcaagtcga gcctctgttg atcataagat tcagcgtacc
     8041 ttgttcgaat aaattgttta aagttcccga atccattgga acccaatcct atcacagctt
     8101 tatatgcatt gcttagacgt tcgtcagtta tatcgaattg ccactggtca ttggtccaat
     8161 cgtcacaatc ttctgtaaca catccaccgg ctatgcctga atttggat
//
LOCUS       DAOQHY010000137         8156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_4507_length_8156_cov_6.030243, whole genome shotgun sequence.
ACCESSION   DAOQHY010000137 DAOQHY010000000
VERSION     DAOQHY010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8156
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4507_length_8156_cov_6.030243"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PLL28_10950"
     CDS             complement(<1..685)
                     /locus_tag="PLL28_10950"
                     /inference="COORDINATES: protein motif:HMM:NF024918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HNF69886.1"
                     /translation="MSAPKPLNILIAPLDWGLGHATRSIPIIKACVDRGHTVVIASTG
                     RSLALLRREFPELTCLDVPAYDIAYQEKGSFILKILGQLGKIFSGIRREHRATKEIVR
                     LHQIDLVISDNRYGFYHKKIHSVIITHQMMVKIPSFVIAEPFVYGWLQLQHKRFNTIW
                     IPDVAGEANISGDLSHKYPVLKRTHYIGILTRFSRPLSLPAAEKTILAIISGPEPQRT
                     LFERLIVTQG"
     gene            758..931
                     /locus_tag="PLL28_10955"
     CDS             758..931
                     /locus_tag="PLL28_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69887.1"
                     /translation="MKNGKKLLWLGAITIMLITTSATCEGGGKKCDCPKFSQIGPNNN
                     DAVASQAAANTLD"
     gene            1002..1511
                     /locus_tag="PLL28_10960"
     CDS             1002..1511
                     /locus_tag="PLL28_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HNF69888.1"
                     /translation="MLTKKVEAALNKQVEMEGYASNYYLSAASWCDSKGLQGAAGFLY
                     GHADEERMHMMKLFHYINDAGGHAEAPNVKQPPLKFKNLKELFTLVFKHEVEVTKAIN
                     NLVDLCMGEKDHSSYNFLQWYVAEQHEEEKLFRTVLEKIELLGPDDTRGLYWIDRELA
                     ALTAAPSAE"
     gene            1591..2649
                     /locus_tag="PLL28_10965"
     CDS             1591..2649
                     /locus_tag="PLL28_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69889.1"
                     /translation="MNTTTVNYLNIALMLLSAIVAFIIPFELFLFSYAVLGPLHYLTE
                     IGWLHKRKYFSPKKNDYIPLVIITLLIAIPAIISYMFVHNGERTSTGQLRLTEGQHNF
                     IAFFQDQNTATALMFLAFSISLIFILIRNNTHRTIAVGLLAIIGFLIRSNHFVDVLFA
                     MFLPTLIHVFLFTGAFILVGALKSKSTSGYLSILVFLGCSLSFFLIMPDGGGYHISDY
                     AKGSYDVSFFGLNHQLFSSFLHESNPTNDMIYNSSTGILITRFIAYAYTYHYLNWFSK
                     TSVIKWHDVPKGWLVTIGVLWVSAVTLYYINYETGLMCLYFLSFLHVVLEFPLNFQSF
                     KQIGEQVRLRLSGKSAAT"
     gene            complement(2751..3287)
                     /locus_tag="PLL28_10970"
     CDS             complement(2751..3287)
                     /locus_tag="PLL28_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69890.1"
                     /translation="MIGTILLVIAGIFALLIIAGLFVPRSYKFSKSIVISGDKKHIFN
                     TIDDLQTWSKWSQWSPDRDPAITITCSNPSAGVNSFMEWKGKKMGAGKMEIVATEPYK
                     NITVATAFNKGLFKMDFDFSLEEIKDQQYSVKWTVRGKTKRGGFAKILGRMLPKMMGK
                     DMEIALKLLKHYCEGTLS"
     gene            complement(3328..4110)
                     /locus_tag="PLL28_10975"
     CDS             complement(3328..4110)
                     /locus_tag="PLL28_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69891.1"
                     /translation="MHIRKFFATMLFVASGMMLHAQTTNTIPNNLDKPDIIKFNLSGA
                     VFGNYTLQYEHVLNVKQSVGMSISFAPNMDLPFKNALVNIFEGNDQAITAINSTQLNT
                     FSITPEYRFYSEVKGAPFGVYGASFIRYTHMTHRQIYTFNLSDGEHHPDVETTFNGVG
                     VGEMVGVQWKIGNSMTLDWWILGPFIGYQTGKSHGDDDRPITPEDKIKLENDIESVPI
                     PGWNIDATVTDNGTTGLGVVDADLSGLFLGARVFGICLGYRF"
     gene            complement(4218..7046)
                     /gene="uvrA"
                     /locus_tag="PLL28_10980"
     CDS             complement(4218..7046)
                     /gene="uvrA"
                     /locus_tag="PLL28_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNF69892.1"
                     /translation="MKTKKSYPNGHIDAIFIKGAREHNLKNIDVAIPRNRLVVVTGVS
                     GSGKSSLTFDTLYAEGQRRYVESLSAYARQFLNRLDKPDVDYIKGIAPAIALDQKVTT
                     RTTRSTVGTLTEIYDYMRLLYARVGRTYSPISGREVKKDEVNDVVDYICSLPENSRIY
                     LLVKLHVDKDRPLNDQARVLQQKGFTRFYANGSIVKIEDILEDPKLIAPKTPVHLLID
                     RVVLAKERAEGSNVPEVNEETRQRIADSVDIAFSEGHGACHVVAEGIEERSFSNNFEA
                     DGMQFEVPSPHFFNQNNPYGACKRCEGFGNVIGIDHDLVIPDKNLSVYQGAVACWKGE
                     KMRTWLDDLLKAAKKFDFPIHKPISQLTKAQYQLLWTGNEHFWGLDRFFKMLEENAYK
                     IQYRVMLARYRGKTTCPECNGSRIRPDADYVLINNMSITKLVDMQVEDLAVFINDITL
                     NAYETKVASRILLEIRNRIVYMLDLGLGYLTLNRRSNTLSGGETQRINLTRTLGSNLT
                     SSLYILDEPSVGLHPRDTERLVTVLKRLRNLGNTVVVVEHEEDVIRNADHIIDIGPAA
                     GIHGGKVVSEGNLEEILADKESLTGNYLSSTMQIPLPRTRRKSFKKLRIRQASMHNLK
                     QIDVTIPLQNLVAVSGVSGSGKTTLIKHILYPELMNMLDDASDTAFISRVIEGDVSAI
                     TQVELVDQDPIGKSSRSNPVTYVKAYDEIRDLYARQPLAKMRGYKSGFFSFNVDGGRC
                     EMCKGEGEIIVEMQFLADVHLVCEDCNGKRFKEEILEVQYKNKNISELLGLSVEEAIH
                     FLQDEKDIVAKLQPLADVGLGYVKLGQSSSTLSGGEAQRVKLASFLGKGKSKEHILFI
                     FDEPTTGLHFHDINKLMDAFNALIECGHSVLVIEHNLEVIKCADYMIDLGPEGGDKGG
                     YLLYQGAPEGIVTVKESYTGQHLKNKLK"
     gene            7219..7797
                     /locus_tag="PLL28_10985"
     CDS             7219..7797
                     /locus_tag="PLL28_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNF69893.1"
                     /translation="MQSRTLSDDKLVELYLTGEESALGELLNRHKDRIYTYIYLFVRD
                     THTAEDLLQETFIKVIDKLRSGKYQEQNKFLPWISRVAYNLCIDYYRREKRMPKVVTR
                     DGFDIFSVLKFSEPGAEGKVMESEFSSRVRKLLEYLPEEQREVVVLRHYMDMNFREIA
                     EMTGVNINTALGRMRYALINLRKLMETKNISI"
     gene            7865..8104
                     /locus_tag="PLL28_10990"
     CDS             7865..8104
                     /locus_tag="PLL28_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69894.1"
                     /translation="MIKTITQDNLIRFIYRETTAEEASLIEEALRSDFDLKELYEQML
                     ESKAELDKVRLQPSQKVIDNIMNYSKNSAHMEHLL"
BASE COUNT         2254 a         1791 c         1644 g         2467 t
ORIGIN      
        1 taccttgtgt aacaattaat cgctcaaata aagtgcgctg aggttcaggc ccgctgataa
       61 tggcaagaat tgttttttct gcggcaggta atgacaatgg tcttgaaaac cgggttaaaa
      121 taccaatgta atgtgtgcgt tttagaaccg ggtatttatg cgaaagatcg ccgctgatat
      181 ttgcctcgcc tgcaacatcg gggatccaga tggtgttaaa tcttttgtgc tgcaattgta
      241 accatccata tacaaatggc tctgcaatca caaacgaagg aatcttcacc atcatctgat
      301 gtgtgatgat aactgaatga atttttttgt gataaaatcc atagcgattg tcggatatca
      361 ccaggtctat ttgatgcagc ctaacaatct ctttggttgc ccgatgttct cttctgatgc
      421 cactgaatat tttacctagt tgtcccagga tttttaatat gaaactgccc ttttcctggt
      481 aggcgatgtc gtaagcaggc acatcaaggc atgtcaattc aggaaattct cttcgcaaca
      541 atgccagcga ccttccggtt gaagcgataa ctaccgtatg gcccctgtca acacacgctt
      601 tgataatggg aatactgcgt gtagcatgcc ctaaacccca gtcgagtggt gctatgagga
      661 tgttaagtgg ttttggtgct gacaaggcaa tattggttaa gaaggctacg tttttactga
      721 cgtattgctg aaatccttaa attagagccg caaagctatg aaaaacggta aaaaactcct
      781 ctggttgggc gcgataacca tcatgctcat taccacttcg gctacctgtg aaggtggcgg
      841 caagaagtgc gattgcccta aattcagcca aatcggcccg aataacaatg atgccgtggc
      901 gagtcaggcg gcagcaaata ctttagactg agtataaatt cggcccttaa cgaactgtct
      961 tcgagcagta atgtttatca ttgcatataa agaagacaac tatgttaacc aaaaaagtag
     1021 aagcggcact taataaacaa gtggaaatgg aaggctatgc ctccaattac tatttatcgg
     1081 cagcatcttg gtgcgattcg aaaggattgc agggagctgc aggctttctt tatggtcatg
     1141 ctgatgaaga acgcatgcat atgatgaaac tctttcatta tatcaacgac gcgggcggac
     1201 atgccgaagc gccaaatgtg aagcagccac ctctgaaatt taagaacctc aaggaactgt
     1261 ttaccttggt gtttaaacat gaggttgaag taaccaaggc cataaacaac cttgtagacc
     1321 tttgtatggg cgaaaaagat cattcttcct acaattttct gcaatggtat gtagctgaac
     1381 aacatgaaga agaaaaactc ttccgtaccg tgctcgaaaa aattgaattg ctgggtcctg
     1441 atgataccag aggtttatat tggatcgacc gcgaactggc tgcattaact gccgctccta
     1501 gcgcagaata attgcaatca cacatcatta aagggcattt ctgcaccatt cgattagcag
     1561 agatgccctt aatcatgtac ttttgtcaac atgaacacaa ctacggtgaa ttacctcaat
     1621 atcgcactta tgctgctttc agctatcgtg gcgtttataa ttcccttcga attgtttttg
     1681 tttagttatg ccgttctcgg acccctccac tatctgactg aaatcggctg gctgcataag
     1741 cgcaaatatt tcagcccgaa gaaaaatgat tatataccac tggtgataat aaccttgctc
     1801 atagccatcc ctgccatcat ctcgtacatg tttgtgcaca acggcgaaag aacttccacc
     1861 gggcaattaa gactcactga aggccaacat aattttattg ctttctttca ggatcagaat
     1921 accgcaacag ctttgatgtt tttggcattt tctatctcgc tgatatttat tttaattcgc
     1981 aacaatacgc atagaacaat tgctgttgga ttgctggcaa taatcggctt ccttattaga
     2041 agtaatcatt ttgttgatgt gttattcgct atgtttctgc ctacgcttat tcatgttttt
     2101 ttgttcaccg gagcatttat tttggttggc gcactgaaaa gcaaaagcac gtccggatat
     2161 ttatccatac ttgtattcct tggatgttca ctcagctttt ttctaattat gcctgatggt
     2221 ggaggatatc atatcagcga ttacgctaaa ggttcttatg atgtatcatt ctttggattg
     2281 aatcaccagt tgttcagcag ctttctgcat gaaagcaatc ccaccaatga tatgatctat
     2341 aattcgtcaa caggcattct tattaccaga tttatagcct atgcctatac ttatcattat
     2401 ttaaactggt tcagcaaaac atcggtgatc aaatggcacg atgtgcctaa aggatggctg
     2461 gtgactattg gtgtgttgtg ggtaagtgcc gttacattgt attatatcaa ttatgaaacc
     2521 ggcttgatgt gtttgtattt tttaagcttt ctgcatgtgg tgcttgaatt tccgctcaac
     2581 ttccagtcat ttaaacagat tggtgagcaa gtaagattgc ggctttccgg taaatccgct
     2641 gcaacgtaat atttaattca gatttaaata attgactaaa tcgcaataaa acaaaaccgc
     2701 ccgacatgtt tcagatgccc gggcggtttt attttaaaat tatgttcaat ttatgatagg
     2761 gtgccttcgc aatagtgctt aagtagtttc agcgcaattt ccatatcctt tcccatcatt
     2821 tttggcagca ttcgaccgag tattttcgca aatccacctc gctttgtttt tccgcgaaca
     2881 gtccatttga ctgaatattg ttggtctttt atttcttcaa gactgaaatc aaaatccatt
     2941 ttgaaaagac ccttgttgaa ggcggtggca acagttatgt ttttgtatgg ttccgttgca
     3001 actatctcca tttttcctgc acccattttc ttgcccttcc actccatgaa actgttcaca
     3061 cctgctgaag gattgctgca agtgattgta atagcaggat ctctgtccgg actccactga
     3121 ctccatttcg accaggtttg caaatcatcg atggtattga agatatgttt tttgtcgcca
     3181 ctgatcacaa tagatttcga aaatttatag ctgcgcggaa caaataatcc ggcaatgatc
     3241 aacaacgcga aaattcctgc aatgactaat aaaattgttc ctatcataac tcgccgctat
     3301 ccgaaatatt gaataattat atattaatta aaagcgatat cccaaacaaa taccaaacac
     3361 gcgcgcacct aaaaacaatc cgcttaaatc cgcatctaca acgcccaatc ctgtagttcc
     3421 attatcagta acagtggcat cgatattcca acccggaatt ggcaccgatt caatatcgtt
     3481 ttcgagtttg attttatctt caggtgtgat aggcctgtcg tcatcgccgt gtgatttacc
     3541 tgtttgatat ccaatgaaag gacctagtat ccaccaatcc aatgtcatgc tgtttcctat
     3601 tttccattgc acacccacca tttcgccaac accaacacca ttaaatgtag tttcaacatc
     3661 cggatggtgt tcgccatcgc ttaaattaaa ggtgtaaatc tgacgatgtg tcatatgtgt
     3721 atagcgtata aatgaagcac catatacacc aaatggagct ccttttactt cactgtaaaa
     3781 gcgatactcc ggtgtgatgc tgaaagtatt taactgcgtg ctgttaatag cggtgatcgc
     3841 ctgatcattc ccttcaaaaa tattaacgag tgcgtttttg aaaggcaggt ccatattagg
     3901 cgcaaaactg atagacatgc caaccgattg ttttacattt aatacatgct cgtactggag
     3961 tgtataattc ccgaatacag caccggaaag attaaatttg atgatatccg gtttgtcgag
     4021 attgttggga atggtatttg ttgtttgtgc atgtaacatc atgccgctcg ctacgaagag
     4081 cattgttgcg aagaatttac gtatgtgcat aggagtgagt tttatagtta aataattatc
     4141 aatttgaaaa aagcaagtta gttcattcct taaagatagt catttgccca tgggaaagta
     4201 ataactaaaa tgcgtgatta ttttaattta tttttcagat gttgcccggt atagctttcc
     4261 ttaactgtta ctattccttc cggagccccc tgataaagta ggtatccacc tttgtcgccg
     4321 ccttcaggac cgaggtctat catataatcg gcgcatttta tcacctcgag attatgctca
     4381 ataaccaaga cactatgtcc gcactcgatc agtgcattaa aggcatccat taatttattg
     4441 atgtcgtgga aatgcagacc ggttgtcggc tcatcgaaaa taaataggat atgctccttg
     4501 cttttccctt taccgaggaa actggcaagt tttaccctct gcgcttcgcc accggataaa
     4561 gtgctgctgc tctgtccgag ttttacataa cctaaaccca catcggcaag cggctgtagt
     4621 ttggcgacaa tatctttttc gtcttgcaag aaatgtatag cttcctctac acttaatcct
     4681 agtaattcag agatattttt gtttttatat tgtacctcca gaatttcttc cttaaagcgc
     4741 ttgccgttac aatcttcaca taccaaatgc acatcagcga ggaattgcat ttcaacaata
     4801 atctctcctt cccccttaca catttcgcat cttccaccgt cgacattgaa actgaaaaat
     4861 ccggatttgt acccgcgcat ttttgcaaga ggctggcgcg catataaatc gcgaatctca
     4921 tcataggctt ttacataagt aaccggattg gaacgtgatg attttcctat aggatcctga
     4981 tcaaccaatt caacttgtgt gattgcgctc acatctcctt caatcactct cgaaataaat
     5041 gctgtgtcac tcgcatcatc gagcatgttc attaattcgg gatataaaat atgtttgatc
     5101 aatgtagttt tgcccgaacc gctaacacct gaaactgcaa ctaaattctg caatggaata
     5161 gtgacatcaa tttgtttcag gttgtgcatg gacgcctgac gaatgcgcaa tttcttaaaa
     5221 ctctttctgc gtgttcgagg tagcggaatt tgcatggttg acgacaaata atttcctgtc
     5281 aggctttctt tatcggccaa tatctcttcc agatttcctt cgctcacaac tttgccgcca
     5341 tgaatgcctg cagcaggtcc aatgtcaata atatgatccg cattgcgaat tacatcttct
     5401 tcgtgttcaa caacaaccac cgtgttgccg agattgcgca agcgttttaa aacggttacc
     5461 aatcgctcag tatcgcgtgg atgtagcccg acacttggtt catcgagaat gtatagtgat
     5521 gacgttaaat tactgccgag cgttctggtg agattgatgc gttgtgtttc gccaccgctc
     5581 aatgtgttgc ttcttctgtt aagtgtgagg tatccgagtc cgaggtctaa catataaact
     5641 atccggtttc gaatttccaa cagaatgcgt gaggcaactt ttgtttcgta tgcatttaat
     5701 gtgatgtcat taataaatac agccagatct tctacttgca tgtctacaag ttttgtgatc
     5761 gacatattat taattaatac ataatctgca tcgggacgaa ttcttgaacc attgcattca
     5821 ggacatgttg tttttccgcg atagcgtgca agcattacgc gatattgaat cttgtatgca
     5881 ttttcttcca acattttgaa gaatcgatcg agtccccaga aatgttcatt gcctgtccac
     5941 agtaattgat attgcgcttt tgttaattgc gaaatgggtt tgtgtatggg gaagtcgaat
     6001 ttctttgcag ctttcaacaa gtcgtcgagc caggtgcgca ttttctcgcc tttccaacac
     6061 gcaacagctc cctgatatac cgatagattt ttatcaggaa taaccaagtc atgatcaatg
     6121 ccgataacat tgccaaatcc ttcacaacgc ttgcaggcgc catagggatt attctgattg
     6181 aaaaaatgtg gcgatggcac ttcaaactgc ataccgtcag cctcgaaatt attactgaaa
     6241 ctgcgttctt caattccttc agctaccaca tgacaggcgc catgtccttc cgaaaaagca
     6301 atatcaacgg aatcggcaat gcgttgacga gtctcctcat tcacttccgg aacatttgaa
     6361 ccttcagcgc gctctttcgc caacactacg cgatctatca gcagatgcac gggtgttttc
     6421 ggtgcgatca gctttggatc ttcgagtata tcctcaattt ttacaatgct tccgttggca
     6481 taaaagcgcg tgaaaccttt ttgctgtaat actctggcct gatcgttgag cggtcggtct
     6541 ttgtctacat gcaatttgac taaaaggtat atcctactgt tttctggaag ggagcagata
     6601 taatctacaa catcatttac ttcgtccttt ttcacctctc gccctgaaat tgggctatag
     6661 gtgcgtccta cgcgcgcata cagcaggcgc atgtagtcgt aaatctccgt cagggtgcca
     6721 acagtactcc tggtggtgcg tgtagtaacc ttttggtcca gggctattgc cggggcaatt
     6781 cccttgatat aatctacatc cggcttgtcc aggcgattga ggaattgtct ggcataggcg
     6841 ctcaaactct ctacgtaccg gcgttggcct tctgcataga gggtatcgaa ggtaaggctt
     6901 gacttgcccg atccgctcac accggttacc accaccaggc ggttcctggg tatggcaaca
     6961 tcgatgtttt tgaggttatg ctcgcgggcg ccttttataa atattgcgtc aatatgaccg
     7021 tttggatagc tttttttcgt tttcaaaccc cttttcttga aactttttta ataaaatatc
     7081 gtaaatctta accaattccc tttgaaaata tcaaatgaag tgtatatttg ttgtgcaaat
     7141 ctcaccaaaa ttagctgcct ataaggttaa atctacgcga aaagaatagt ttattcattt
     7201 aacgcacaac cttttagtat gcagtccaga accctttctg atgacaagct ggtcgaattg
     7261 tacctgaccg gcgaagaatc tgccctcggt gaactcctca accgtcacaa agataggata
     7321 tacacgtaca tctatctttt tgttcgcgac acgcacactg ctgaagacct cttgcaggaa
     7381 accttcatca aggtgattga caagctacgg tccggaaagt atcaggaaca gaacaaattt
     7441 ttgccatgga tatctcgcgt ggcgtataac ctttgcattg actactatcg acgtgaaaag
     7501 cgcatgccca aagtggttac ccgcgatggc ttcgatattt tcagtgtgct gaagttctca
     7561 gaacccggcg cagaaggcaa agtgatggaa tcggaattca gcagccgcgt ccgcaaattg
     7621 cttgaatacc tgcctgagga acagcgcgaa gtggtggttt tgcgccatta tatggacatg
     7681 aacttccgcg aaattgccga aatgacgggg gtaaacatca atacagcctt gggacgtatg
     7741 cgctatgcac tcatcaacct gagaaaactg atggaaacga aaaatattag catttgatat
     7801 actaacgcaa tctgcatgaa cgttttaggt ctgttctttt atggttaaac ctaaaacaaa
     7861 ttgcatgatt aaaaccatta cacaagacaa tctcattcga tttatctatc gcgaaacaac
     7921 agcagaggaa gccagcctca ttgaagaggc gctgcgcagc gacttcgatc tgaaggaact
     7981 gtatgagcaa atgctcgaaa gcaaggctga actcgataag gtccgactgc aaccttcgca
     8041 aaaagtgatc gataacatca tgaactacag caagaactcc gctcacatgg agcacctgct
     8101 gtaaaagaga tgaaaaaacc ccgatctgac cagatcgggg tttttacttt atttat
//
LOCUS       DAOQHY010000138         8153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_4511_length_8153_cov_6.365893, whole genome shotgun sequence.
ACCESSION   DAOQHY010000138 DAOQHY010000000
VERSION     DAOQHY010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8153
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4511_length_8153_cov_6.365893"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3446
                     /locus_tag="PLL28_10995"
     CDS             <1..3446
                     /locus_tag="PLL28_10995"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNF69895.1"
                     /translation="INGILISEAGLTGIPAYDDLSVTAHEAQMFDGGNPAYITLDSAT
                     LAGIIVDGTNVIAVQVHNKTSGSNDLTGRTFLHFAITDASVFYGIPPLWFNPTNSTEL
                     HTNFKIGLGETIALFDNAGILRDSITMQDLEAGIVKARIPDAGGWCMTNIPTPDTVNS
                     GDCYLLYAATPSITPLPGFYTGDQTVTISGVDVQYTTDGSDPLLGGGISYIDPFVVTT
                     STVIKARSFETGKLPSALATTSLLINEPTLLPVVSISATPCDLFDLAATCPAAYDNAT
                     SFDNENPQINCVIEYFNADKTFQFAAPMKFEVAGNYSIELDQKGIQFTCDEDFNSPDN
                     INFPLYSLDKPVIDKYHAFRLRNCDNDAELARSRDLLINRMALPTHTVAAANQNVAAF
                     INGEYWGHYVSRERLDPYFCRDNFGANPDRVDMIKAHFSGGGNYVTEAGSDTGFFNLH
                     TYCIDHDLTDPVYYAAVLDQIDEDNWIDYFATEVYSANSDWIGFIVNNIRLFRAVSPD
                     IEWKFMLWDLGSSQGTPGAGASSNTLATAMDDNNFYIDMFNALMENTEFHDHFINRFA
                     DLLNYYYTPEIANALIDANTAEIITEMNAQDDKWNTGDSSFVQSKITNLRNFHNDRRG
                     YVRDDIENYYDLNDQVDVTIDVAPAGAGYIKISTIIPETLPWTGVYFDGVPVTITAIA
                     NPGYTFVNWTDNPFIATADVPAFNVNITEATTFTANFNGTGISNPIIISEINYNSDST
                     LNAGDWIELHNTSGMEIDLTNYQFSNTIFYNDYIFPSIKIAPDGYLVLAEDMIEFNAV
                     YPDVTQVIGGFDFNLANDGDSITLIDFLGNVITSFKFNDKRPWPVTADNYGRTLERFE
                     PAIDPGLPEAWFPGCVGGSPAAAFTSCLENPLVDEINYNSDADSNAGDWFELMNTSAD
                     IIDIGNWTVKDKNGNAYVLPTGTQILPDSFIVIYQDAALFASRFPDVTNISGPTGFGL
                     SSDDDIISIYDAGGVIYQSVSYDDTIPYPISPDGGGVTLQIVDVLENINLASNWMESC
                     PEGSPGMDYYFPCLVHEDTTTDTSGEDTTIVESIANYLEGDILIFPNPANAQINISLP
                     DNLQTDGLMHVYDLAGTLKETFDVSASTMQLDISNYLPGVYLIQWINDTSQLQQAFIK
                     E"
     gene            complement(3447..3842)
                     /locus_tag="PLL28_11000"
     CDS             complement(3447..3842)
                     /locus_tag="PLL28_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HNF69896.1"
                     /translation="MMHSPEIPTDRQTWESPIAYYWWEDEILISLSKSTLRTVETIKA
                     NVAIVQTITGNKPTPLLIYLSKSPIPDKETQSYSKTQLPLIYSSMAMVSKPGLAQFIM
                     RLLFSFQKPPIPMKSFADAEKAKRWLRSH"
     gene            complement(3846..5354)
                     /locus_tag="PLL28_11005"
     CDS             complement(3846..5354)
                     /locus_tag="PLL28_11005"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HNF69897.1"
                     /translation="MQFTYRNGILKKYSILTLVLSMTCISMNGQGNGEAHRPLIHFTP
                     ETGWMNDPNGLIYYDGYYHMFYQYYPDSTVWGPMNWGHAQSLDLMTWERQPIALYPDS
                     LGWIFSGSAVIDVHNTAGFGNNAMVAIFTYHNDAIWQSGNKNTESQGLAYSTDKGATW
                     KKYVGNPVLNNTGEQDFRDPKVFWNSSIRKWNMVLAVGDHIRIFSSPDLKKWTFESDF
                     KPEEDEEVGVWECPDLFQMAVAGSSDKKWVMLMSHGYNEKDNKQSGTRYFVGDFDGHT
                     FKNTQQAQWIDVGSDFYAAATYANAPGNKRILQAWMSNWMYAQEVPATRWRSTMTLPR
                     ELALIKDKYVYAITQQWATQIINNQSEIYSKPESSDVELSGNVIPHCRITLDLEENNT
                     TSIQLSNNENEVFTILIDGTTITTTRDVYRDEDAIYPFITETAASLPGERITKLDMIF
                     DVGSCEILVNDGKLSMTTIYFPTSNFDKLSINPMDGGILRNVTIYGITPTMW"
     gene            5528..7300
                     /locus_tag="PLL28_11010"
     CDS             5528..7300
                     /locus_tag="PLL28_11010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69898.1"
                     /translation="MDPTFDGDGIVTTAFADGYDVIFAMELQPDGKILGAGGTNFIAG
                     TETDFVLLRYLTDGSLDESFGDGGIVTTDFELAHDLANAMYLMPDGRILAAGYSGNDW
                     AIARYLPDGELDTTFSNDGKLLVDFDLENDQAFGIATQSDGKIVVGGFATVDGFYPRF
                     ALARFYENGDPDLSFGVDGKTTETVFGRWDQARALILRPDDRIMLVGYTQDLMNSFDM
                     TVMQFNSNGALDVSFGDDGIVRTDFDSTVNFAYCGMLQPDGKLVIAGTTDVVPDQNIA
                     MARYLTNGELDTTFGDGGRVVINVDPYQEEQIFGIAYEPDGRIIIGGEIGTYADGYDY
                     LVAKLLPDGSPDSTFSDDGIVITHLGDDWDLAYDVLIQDDTKILAGGFTNDAGDNDFA
                     VLRYFNSPYITCDTITEQPMDVIILPLATAVFNVTSPFPDAMYQWQEWDGVAWTSLED
                     VYPYSGTHTSELSIAPVDYWYDGLQFRCIVAYDICSDTSNAALLVVLLDDINELQQNL
                     SFSIFPDPVQSILNVGYTNDYATSIKWQICNALGAVCLKGDEYLEKGTDVFQINTLEN
                     LPAGIYTCSISVDGVRNTKSFIKN"
     gene            complement(7307..7852)
                     /locus_tag="PLL28_11015"
     CDS             complement(7307..7852)
                     /locus_tag="PLL28_11015"
                     /inference="COORDINATES: protein motif:HMM:NF025675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HNF69899.1"
                     /translation="MRLTYRLSEQDFLNCHLFFTSRSEATRKARKRNRLLMPGIYIVL
                     GLATLFIGDPILSGLLIVAGVLWYFFFPGYEGKRIATRMQKHLQENLRSQFDQETNLD
                     ITKEMIIDKVNTLESRIPASEIKEIYKVPGYYYFLTKTGTAIIIPRNILEDESTLNGV
                     IQEMCDDLKLPYREFAEWKWK"
     gene            complement(7880..>8153)
                     /locus_tag="PLL28_11020"
     CDS             complement(7880..>8153)
                     /locus_tag="PLL28_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69900.1"
                     /translation="CQVLSIHSPTMHGVTLYPNPVDDILMIAGTIDNIVSVSVVNTIG
                     VELFCQQSCTDLNYVLTTASLAAGNYYCICKSDIGTRIILPFIVEH"
BASE COUNT         2259 a         1562 c         1744 g         2588 t
ORIGIN      
        1 ttattaatgg cattttaatt agtgaagccg gcctaacggg aatacctgct tatgatgact
       61 tgtcggttac tgcacatgaa gctcaaatgt ttgatggtgg taatcctgca tatattacgc
      121 ttgattcagc aacactagcc ggaattattg ttgatggtac gaatgttatc gctgtccaag
      181 tgcataataa aacatcaggc tcaaatgacc tcacagggag aacatttttg catttcgcta
      241 tcacagatgc ttctgtgttt tatggaattc cgcctctgtg gtttaaccca acgaattcaa
      301 ccgaattaca tacaaatttc aaaataggac ttggtgaaac tattgcgtta ttcgataacg
      361 caggtatttt gcgtgatagc attaccatgc aagatttaga agcaggaatt gtaaaagcaa
      421 gaattcctga tgcaggtggc tggtgtatga caaacatacc gactcccgac accgtgaatt
      481 ccggtgattg ctatttgcta tatgctgcca cgccatccat tacaccatta cccggattct
      541 ataccggcga tcaaacggtt accatttcag gggttgatgt gcaatataca actgatggaa
      601 gcgatccttt attaggagga ggtatttcct atatcgatcc gtttgttgtt actacatcta
      661 ctgttatcaa agcaagatct tttgaaacag ggaaattgcc aagtgcgctg gcaaccacca
      721 gtctgttaat taatgagcca acattattac ctgtagtaag tatcagtgcc acaccgtgcg
      781 atttgtttga tcttgccgcc acctgtcctg cagcgtatga taatgctaca tcattcgaca
      841 atgaaaatcc ccagatcaat tgtgttatcg aatactttaa tgccgataaa acatttcaat
      901 tcgctgcacc aatgaaattt gaagtggcag ggaattattc tattgaattg gatcagaaag
      961 gaattcagtt tacttgtgat gaggatttta atagccccga taatatcaat ttcccattgt
     1021 acagcctgga taaacccgta attgataaat atcacgcatt tcgcttgcgc aattgcgata
     1081 atgacgcaga gttggctcgt tcgcgcgact tgctgattaa caggatggca ttaccaacgc
     1141 atactgttgc agctgcaaac caaaatgtgg cagcatttat caacggcgaa tattggggtc
     1201 attatgtttc tcgagagcgt ttagacccat atttctgtcg cgataatttc ggtgcaaacc
     1261 ctgatagggt agacatgata aaggctcact ttagtggcgg tggaaattat gttacagagg
     1321 cgggaagcga tacaggtttt tttaatctgc atacttattg tatcgatcat gatctgacag
     1381 atccggttta ttatgctgca gtattggatc agattgatga agataactgg atagattatt
     1441 ttgcaactga agtgtatagc gctaactccg attggattgg atttattgta aataatatac
     1501 gtttattcag agcggtatcg ccggatattg aatggaaatt tatgttgtgg gaccttggat
     1561 catcgcaagg tacacccggt gccggagcaa gttcaaatac acttgcaacg gctatggatg
     1621 acaataattt ttatattgat atgttcaatg cgttgatgga gaacaccgaa tttcatgatc
     1681 actttattaa tcgctttgcc gatttgttga attattacta tacccccgaa attgccaatg
     1741 cattgatcga tgcgaataca gccgaaataa tcaccgagat gaatgcacag gacgacaaat
     1801 ggaataccgg cgattcgagt tttgtgcaaa gcaaaatcac taatctgcgc aattttcata
     1861 acgacagacg tggttatgtg cgagatgata tagaaaatta ttatgacctt aatgatcagg
     1921 tagatgtgac tattgatgta gcacccgccg gagccggata tatcaaaata tcgacaatta
     1981 ttcctgaaac tttgccttgg acaggtgtgt attttgatgg tgttcctgta acgattactg
     2041 ctatcgccaa cccgggctat acttttgtta actggacgga taatccattt attgcaacag
     2101 cggatgtgcc tgcatttaat gtgaatatta cagaggcaac aacatttact gccaatttta
     2161 atggcacagg tatttccaat cccatcatta tttctgaaat caattataat tctgacagca
     2221 cattaaatgc cggcgattgg attgagttgc ataatacttc cgggatggaa attgatttga
     2281 ctaattatca attctccaat accattttct acaatgatta tatatttcct tccattaaaa
     2341 tcgcgcctga tggatattta gtgctggcgg aagatatgat tgaatttaat gccgtttatc
     2401 ctgatgttac tcaggttatt ggcggatttg atttcaacct tgccaatgat ggagattcca
     2461 ttacgctaat cgattttctt ggtaatgtta tcacatcatt caaatttaat gataagcgac
     2521 cttggcctgt aactgctgat aattacgggc gaacgcttga acgctttgaa cctgcaatag
     2581 atcccggttt acctgaagct tggtttccgg gttgcgtagg tggttcgccg gctgcagctt
     2641 ttacatcatg tctcgaaaat ccactggttg atgagatcaa ctataattct gatgctgatt
     2701 caaatgcggg cgattggttt gaattaatga atacaagtgc ggatataatt gatattggta
     2761 attggaccgt aaaggataaa aatggaaatg catatgtgtt accgacaggt acccaaattt
     2821 tgcccgacag ttttattgta atttatcagg atgcagccct tttcgcatcg cgctttcctg
     2881 atgtgactaa tatttccggg cctacagggt ttggactcag cagtgatgat gatatcatca
     2941 gtatttatga tgcaggtggc gtgatttatc agagcgttag ttatgatgat acgataccat
     3001 atccaatttc ccctgatggt ggaggcgtga cattgcaaat tgtagatgtg ttggagaata
     3061 tcaaccttgc ttcaaattgg atggagagct gtcctgaagg ttcaccgggt atggattatt
     3121 attttccttg tctggtccat gaagatacta ctaccgatac ttcaggtgag gacacgacta
     3181 ttgtagaatc gattgcaaat tatcttgaag gtgatatttt aatattccca aatccggcga
     3241 atgcacaaat aaatatatcg ctgcccgaca atttgcaaac tgatggtttg atgcatgttt
     3301 atgatctggc cggaacactt aaagagacat ttgatgtttc tgcttcgacc atgcaattag
     3361 atatttcaaa ttatttgccg ggtgtgtatt taattcagtg gattaacgac acgtctcaac
     3421 tacaacaggc atttattaaa gaataattaa tgactgcgaa gccagcgttt tgctttttcg
     3481 gcatcggcaa aactcttcat ggggattggc ggtttttgaa aactgaacag taaacgcatg
     3541 atgaattgtg ctagtcccgg tttggatacc attgccatgg aagaataaat gagaggtaat
     3601 tgcgttttgg aatatgactg agtttcttta tccggaatcg gcgattttga taaatatatc
     3661 agcaagggcg tgggcttgtt tccggtaatg gtttgaacta ttgcaacatt ggctttgatc
     3721 gtttcaactg tcctcaatgt agattttgac agcgatatca atatctcatc ctcccaccaa
     3781 taataagcaa ttggactctc ccatgtttga cggtctgttg gaatttcagg gctatgcatc
     3841 ataatttacc acattgttgg cgtgatacca taaattgtca cgtttcttaa aattcctcca
     3901 tccatcggat taatggatag cttgtcaaag ttacttgtcg gaaaataaat ggtggtcatg
     3961 gataattttc cgtcattcac taatatttca caggaaccga catcgaatat catatccaat
     4021 ttggtgatgc gctctccggg taagctcgct gcagtttctg taataaatgg ataaatagca
     4081 tcctcgtcgc ggtaaacatc acgtgttgtt gtgatggttg tgccgtctat caaaatggta
     4141 aacacctcgt tttcattgtt tgacagctgt atggaagtgg tattgttttc ttcgaggtct
     4201 aaggttattc ggcaatgcgg aattacattc cctgataatt caacatctga agattcaggt
     4261 ttactgtata tttcactttg attgtttatt atttgagtag cccattgctg cgtgatggcg
     4321 tatacatact tatctttaat tagggctaat tccctaggca atgtcatagt gcttctccat
     4381 cttgttgcag gcacttcctg cgcgtacatc caattgctca tccaggcttg aagaatacgt
     4441 ttattccctg gcgcattagc gtaggttgca gcagcgtaaa aatctgagcc tacatctatc
     4501 cattgtgctt gttgtgtgtt tttaaatgta tgcccatcga aatcgccgac aaaatagcgc
     4561 gtccctgatt gtttgttgtc tttttcgttg taaccatgac tcataagcat cacccatttt
     4621 ttatcactgc ttcctgctac tgccatttga aataaatcag ggcattccca aacaccaacc
     4681 tcctcatctt cttccggttt aaaatcactt tcaaatgtcc atttttttaa atcaggggat
     4741 gaaaatatgc gaatatggtc gccaacagcc aacaccatat tccattttct gattgatgaa
     4801 ttccagaata ctttcggatc tctgaaatct tgttcgccgg tattatttaa aacaggattg
     4861 cctacatatt ttttccaagt ggcacctttg tcggtgctgt atgccaatcc ctgactttcg
     4921 gtgtttttat ttccgctttg ccaaatggca tcattgtgat aagtgaaaat ggcaaccatg
     4981 gcattattgc caaagcctgc agtattgtgc acatcgatga cggcacttcc cgaaaatatc
     5041 cagcccaggg aatcgggata aagcgcaatc ggctgtcgtt cccatgtcat cagatcaaga
     5101 ctttgcgcat gcccccaatt cattggtccc catacggttg aatcaggata atattgataa
     5161 aacatatgat aatatccatc gtaataaatt aatccgttcg ggtcattcat ccaacctgtt
     5221 tccggcgtaa aatgtatcaa aggtctgtga gcctctccat tgccttgtcc gttcattgaa
     5281 atgcatgtca ttgataaaac aagcgtgagg atggaatact ttttaagaat gccgttgcga
     5341 taggtaaatt gcatgtagga gcctttgtgc tataaaaata gtgtaatcaa aattggcgat
     5401 gcaaaatcta aacgaacaaa tgtggtatat ttgatataaa ggttgccgta tgcttctgag
     5461 atctttactc cttttacaat cactgacgtt ctttagagct tgcatttttg cgcaatttcc
     5521 gggtgacttg gatcctacat ttgatggcga tggtatcgta accactgctt ttgccgatgg
     5581 atatgatgtc atttttgcta tggaattaca acctgatgga aagatattag gtgccggtgg
     5641 tactaatttc attgcaggca cagaaacgga ttttgtgttg ttgcgttacc taactgatgg
     5701 atccttggat gaaagcttcg gcgatggcgg tattgtaact actgatttcg aattggctca
     5761 tgatcttgcc aatgccatgt atctcatgcc cgatggacga attctggcag cagggtatag
     5821 tggaaacgat tgggcaattg cgaggtatct gcctgatggt gaattagaca ctactttcag
     5881 taatgatggt aagttgctgg tcgattttga tcttgaaaat gatcaagctt tcggcattgc
     5941 cacccaatca gacggcaaga tagttgttgg tggttttgca actgttgatg gattttatcc
     6001 gcgatttgca ttagcaaggt tttatgaaaa tggcgatccg gatctctcat ttggagtgga
     6061 tggaaaaaca actgaaacag tatttggtag atgggatcag gcaagagcgt tgatcctgcg
     6121 tcctgatgat agaataatgc ttgtcggata tacacaagac cttatgaata gttttgatat
     6181 gacggtcatg cagtttaatt caaatggagc ccttgatgtc agcttcggtg atgatggcat
     6241 agtaagaact gattttgaca gcactgtgaa tttcgcatat tgtggtatgc tgcaacctga
     6301 tggtaaattg gtaattgccg gtactacaga tgttgtgccc gatcaaaata ttgcaatggc
     6361 ccgatatctt acaaatggtg aattggatac tacatttggc gatggcggac gagttgtgat
     6421 caacgtagat ccgtatcaag aggagcaaat atttggtatt gcatacgaac ctgatgggag
     6481 gataattatt ggcggtgaga ttggaaccta tgccgacggc tatgattatt tggtggcgaa
     6541 attattaccc gacggatcac ctgatagcac tttcagcgat gatggtatcg taatcactca
     6601 cttgggtgat gattgggatc tggcttatga tgttttaatt caggatgata ctaagattct
     6661 tgccggagga tttacaaacg atgccggtga taacgatttt gccgtgctga ggtattttaa
     6721 ttcgccatat atcacttgcg ataccattac tgaacagcca atggacgtta ttattcttcc
     6781 gctggctacg gcagtgttca atgtgacttc acccttcccg gatgcaatgt atcagtggca
     6841 ggaatgggat ggtgtagcat ggacatcgct tgaagatgtc tatccgtatt caggaaccca
     6901 tacctccgaa ctgtctattg cgcctgttga ttactggtat gacggccttc agttccgatg
     6961 tattgttgcg tacgacatct gctccgatac cagcaatgca gcactacttg ttgtactatt
     7021 agatgacatc aatgagttgc agcagaattt atctttttca atatttcctg atcctgtgca
     7081 aagtatcttg aatgtaggtt atactaatga ttatgctaca tcaataaagt ggcagatttg
     7141 taatgcatta ggcgcagtgt gcttgaaggg cgatgaatat cttgagaagg gaacggacgt
     7201 ttttcagatc aacacattag aaaatctacc tgccggtatt tatacatgca gtatttctgt
     7261 tgatggtgta aggaacacaa aatcatttat taaaaactga attgatttat ttccatttcc
     7321 attcggcaaa ttcccggtag ggcaatttta aatcatcaca catctcttgt atcaccccat
     7381 tcaatgttga ttcatcctcc aggatatttc gtggtatgat gattgctgtt cctgttttag
     7441 tcaggaagta ataatatccg ggcactttgt aaatctcttt gatctcactt gccggaattc
     7501 tggattccaa cgtattaacc ttatcaataa tcatttcttt ggtgatgtcg aggttggtct
     7561 cctgatcgaa ttgagatcgt aagttttctt gcaaatgctt ctgcatgcgt gtagcgattc
     7621 ttttgccttc atagcccgga aagaaaaaat accataatac acccgctacg atcaataatc
     7681 cacttaaaat aggatcgccg ataaataagg tggcaaggcc caaaacgata taaattccgg
     7741 gcataagcag gcgattgcgt ttcctcgctt ttcttgtagc ttctgatctt gatgtaaaga
     7801 ataaatgaca gtttaaaaaa tcttgctcac tcaggcgata agtcaatctc atgaaatgta
     7861 ctttcctgta aagttacaat caatgttcca caataaacgg taagataatt cgggtgccga
     7921 tgtcgctttt gcaaatgcaa taataatttc ccgctgccaa tgatgcggtg gttagtacat
     7981 aatttaaatc tgtacagctt tgttgacaaa ataattctac tccaatcgta ttcaccacag
     8041 agaccgaaac tatattgtct attgttcctg cgatcatcag aatgtcatca accggattcg
     8101 gatacaaggt tactccatgc attgtcggtg agtgtattga cagcacttga cat
//
LOCUS       DAOQHY010000139         8099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_4562_length_8099_cov_5.298732, whole genome shotgun sequence.
ACCESSION   DAOQHY010000139 DAOQHY010000000
VERSION     DAOQHY010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8099
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4562_length_8099_cov_5.298732"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1564)
                     /locus_tag="PLL28_11025"
     CDS             complement(<1..1564)
                     /locus_tag="PLL28_11025"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNF69901.1"
                     /translation="MASLNLQTPLTFLKGVGEQRAAMLLKELELETCYDLLHFFPFRY
                     IDRTDFTTVKAVKQNAFTLAELREVQMKGFISKMHIVGAKQGRRLIATFSDNTDTMEL
                     VWFNGINWLQKSLKEDVQYIVYGKPSIYNGVVNMMHPEMEPAAQSDFQLSGKIQPIYS
                     GTEKLKSRGINSKMIARFVQSLIAQLSELDLPEQLPASVIQKYKLIGRFEAYKCIHFP
                     DSDAHLQHALRRLKFEELFIDQIKLLRVKQYRHSSSMGFIFKELDTYFNPFYTAHMPF
                     ELTNAQKRVMKEIRKDLLSGRQMNRLLQGDVGSGKTVVALLTMLMALDNGFQACLMAP
                     TEILSQQHIAGISELLKDLPIQVALLTGSVKGKQRKLILEGVADGSIQILIGTHALIE
                     ETVQFKNLGCVVIDEQHRFGVEQRSKLWNKNSLTPHILVMTATPIPRTLSMAYYGDLD
                     VSIIDELPPGRKPIKTVHRKESERLHVFGFLKEQIAEGRQVFIIYPLIEESEKLDLKN
                     LMEIILKSFLLCL"
     gene            complement(1669..2262)
                     /locus_tag="PLL28_11030"
     CDS             complement(1669..2262)
                     /locus_tag="PLL28_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69902.1"
                     /translation="MSLFRTVIFCFALAAIIGCHTDEAPKPDAWPRVHYPVATDTIVF
                     NDEDCPYTFSLPNYYEIDRNVTYFNQTPDDPCWMNLVCKDLNATIYMSNKPIDVKYSY
                     QQMIDDAYKLTYKHAGRADYIEPQEIENQYGVHGLIYYVGGDAASNFQFFVTDTTSNF
                     VRGALYFNNRPNADSLRPVIQFMVHDIENMLSGWRWK"
     gene            complement(2297..2716)
                     /locus_tag="PLL28_11035"
     CDS             complement(2297..2716)
                     /locus_tag="PLL28_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNF69903.1"
                     /translation="MSGVNKVILIGNLGKDPELRNFEGGNVVANFTLATTEVFRDRNG
                     NKVEQTEWHNIAMWGKLAEIAGKILRKGAKVYIEGRIKNRSWEDKEGHKKYITEIVAD
                     NFTLLGPRPANTGGIDHPDDDMNHQHPQSDIQDDLPF"
     gene            complement(2816..3865)
                     /gene="mutY"
                     /locus_tag="PLL28_11040"
     CDS             complement(2816..3865)
                     /gene="mutY"
                     /locus_tag="PLL28_11040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNF69904.1"
                     /translation="MSSFSKTLLQWYRLNKRNMPWVGETDPYKVWLSEVILQQTRVEQ
                     GTAYYLDFIKHYPTVQHLAKASANEVMKRWEGLGYYSRARNMHATANVITQQLKGVFP
                     DTPEGLQSLKGIGKYTANAILAYAYHKPYAVTDGNVLRILSRYQGITIPVNTPAGKKM
                     VEALADRLLDKQLPHTYNQALMDFGSLVCTPRNPDCGNCPFRRSCYAFNHEMTAILPQ
                     KEKKRAAKTRYFNFFVMKSGNKVLIMPRHAADIWKALWQFPLIETAEEATLQDIQRSN
                     VYKDWKLGKVQWEEAGTFKQQLTHRRLICRFFTGEIKTTPEIDGMQELVNRKEMERFA
                     FPGVIRDFMKGKLYF"
     gene            3971..4270
                     /locus_tag="PLL28_11045"
     CDS             3971..4270
                     /locus_tag="PLL28_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNF69905.1"
                     /translation="MRKADIVNRITDKTGLEKVDVMVVVEAFCKEIKESMAEGHNVYL
                     RGFGSFVVKTRKKKIGRIIKKNIAIEIPEQKIPSFKPAKLFLNRVKDGKKSKSGL"
     gene            4271..5059
                     /locus_tag="PLL28_11050"
     CDS             4271..5059
                     /locus_tag="PLL28_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69906.1"
                     /translation="MKKYLIPAGIAVVLILVIFFAFDHKPPKVSVFDERREKLIDSLQ
                     YPNSEDLMHMHRDMLHAGGGTKAALLQQLADKWMQLAQPSIAADYIRQLADADPSYEH
                     YMQAGAAMGSLIDFEAEEDLRMSLVYGARYSYEMAEKLQPGDLDARIGLASVLVSGGS
                     TPMEGIMMLREMDAENPDNIRINLELGKFSVMSGQFDKALERFDRVLQKDSLNLQARY
                     MRAQSFLGIQDTAKAVMELETLKSQTTDPTLIEQVDNEIKNLNH"
     gene            5267..6820
                     /locus_tag="PLL28_11055"
     CDS             5267..6820
                     /locus_tag="PLL28_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNF69907.1"
                     /translation="MERELIINTTPTGAEIALLEDKQLVEIHRDSFSSRFNVGDLYLG
                     RVKKITPGLNACFVDVGFERDAFLHYTDLNPQFKSIYKYTKAAQDGRAYSMDKFQREP
                     DIVKTGKINNVISKSQHILVQIIKEPIQTKGPRLSCEISLPGRYLVLTPFNEVVGVSR
                     KIASGEERKRLQKAIEQLRPKGFGVIIRTVAENVPVQKLHDDLNNLVNQWKEITNRLQ
                     SAQAPQLIFSESKKTNTLLRDVLNDSFNRVVVNDNAIQTEVKDYIRRISPGSEKMVLM
                     HSGPKSVFEQFGVTRQIKGLFGRTVNMDSGAYLIIEHTEALHVIDVNSGNKSAVKGDQ
                     EANAVSVNMEAAREIARQLRLRDLGGIIIVDFIDMKNPENKKAVYNALKNAMAGDRAK
                     HTILPISKFGVAQITRQRVRPEVNISTAEVCPTCNGTGKIEASILLLEDIERKLKHLV
                     TIQNLKAVNLMVHPYVAAYLKKGWFFRSLYWQWRRTYKVKLKVTSSDDFHYMEYRFFD
                     LNEGEINVE"
     gene            complement(7062..>8099)
                     /gene="nadE"
                     /locus_tag="PLL28_11060"
     CDS             complement(7062..>8099)
                     /gene="nadE"
                     /locus_tag="PLL28_11060"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNF69908.1"
                     /translation="GIPVFYVNHCGAQTDLIFDGGSLVINANAVVYDEMPYFTECIKV
                     YDLDDVLASSKTLNTDIVQEKDTTALIYQALVTGIRNYFEKLGFKKAILGLSGGIDSA
                     LTAVLAVHALGKENVWGLLMPSQYSTDHSIKDAEDLAKNLGMRFDIVPIGNMFNAVES
                     QLQPLFNNKPEDVTEENLQARLRGLTLMAMCNKHGNILLNTTNKSEAAVGYGTLYGDL
                     CGGLAVLADVYKTQVYTLSNYINRNEEIIPLHSITKAPSAELKPGQKDQDTLPPYDIL
                     DAILYQYIELHQSPQDIIEKGFNPAIVNRTLKMVNSNEWKRWQFPPILRVSPKAFGTG
                     RRMPIEGKYLS"
BASE COUNT         2228 a         1636 c         1846 g         2389 t
ORIGIN      
        1 ttaagcaaag gagaaagctc ttcaagataa tttccattaa atttttcaaa tcgagttttt
       61 cactctcttc aataagtgga taaataataa atacttgccg accttcggca atttgttctt
      121 tcagaaatcc aaatacatgc aagcgctcac tctcttttcg atgcactgtt tttatcggct
      181 tgcgacccgg tgggagttcg tcgatgatgc taacatccag atcaccataa taggccatgg
      241 aaagtgtgcg gggaataggt gttgccgtca tcaccagaat atgcggcgta aggctgtttt
      301 tattccacaa tttcgatcgt tgctccacac caaatcgatg ttgctcatct attacaacac
      361 aaccaagatt tttaaattga actgtctctt caattaatgc atgtgtgcct attaaaatct
      421 gtatactgcc atcggcaaca ccttccagaa ttaattttcg ctgcttgcct ttcacactgc
      481 ccgtaagtaa tgcaacctga atggggagat cttttaatag ttctgatatc cctgctatat
      541 gttgttgcga taatatttct gtaggcgcca ttaaacaagc ctgaaatcca ttgtcaagtg
      601 ccatcaacat ggtgagcagt gctaccactg ttttaccgct acccacatcg ccctgcaata
      661 atcgattcat ttgtcgacca ctcagcagat cttttctgat ttccttcatc acccttttct
      721 gtgcattggt gagttcgaag ggcatgtgtg ctgtgtaaaa cggattgaag tatgtatcta
      781 attccttaaa aataaatccc atgctgctgc tatggcgata ttgttttacg cgcagcaatt
      841 ttatctggtc gataaataat tcttcaaatt tcaatcgccg caaggcatgt tgtaaatgcg
      901 catcggaatc gggaaaatgt atgcacttgt atgcttcaaa tcgacctatc aatttatatt
      961 tctgaatgac ggaagcgggc aattgttcag gcaaatctaa ttccgataat tgtgctatca
     1021 gcgactgcac aaatcgggct atcattttac tattgatacc gcgacttttt aatttttcgg
     1081 tacctgaata tatcggctga atttttccac tgagttgaaa atcactttgt gctgcaggct
     1141 ccatttcggg atgcatcata tttactacgc cattataaat ggaaggcttt ccgtaaacaa
     1201 tatattgcac atcctccttc agcgatttct gcaaccagtt gatgccattg aaccatacca
     1261 actccatggt atcggtattg tcgctgaaag tggcaatcaa tctccgccct tgtttagcac
     1321 caacaatatg catcttgctg ataaaccctt tcatctgcac ttcgcgcaat tcagctagcg
     1381 tgaaggcatt ttgcttaacc gctttaacag tagtaaaatc ggtgcgatcg atgtatctga
     1441 acggaaaaaa atgcagcaaa tcatagcagg tttccaattc cagctccttc agcaacattg
     1501 ctgcacgttg ttcgcccacg cctttgagaa aggtgagtgg tgtttgcaga ttcaggctag
     1561 ccacgagata atttaagcaa tcggaaagtt agcaaataaa ataattattg aagtttagta
     1621 tcgtcaatca ataattatgc agcacacatt tgttatcgcc cacaaacatc acttccacct
     1681 ccaaccgctc aacatatttt caatatcgtg caccataaat tgaatgacgg gtcttaatga
     1741 atctgcattt gggcggttgt tgaagtataa tgctccacga acgaaattgc tggtggtatc
     1801 ggttacgaaa aattgaaaat tactcgctgc atcaccacca acatagtaga tcagtccatg
     1861 tacaccatac tgattttcta tttcctgtgg ttcgatataa tcagctctgc ctgcatgttt
     1921 gtatgtgagc ttgtaggcat cgtcgatcat ctgttgatag gaatatttta catcaatagg
     1981 tttattgctc atgtaaatag tggcattcaa atccttacac actaaattca tccaacacgg
     2041 atcgtcaggt gtctgattga aataagtaac attgcgatct atttcgtaat aatttggaag
     2101 tgaaaatgta taagggcaat cttcatcatt aaacactatc gtatccgttg caacaggata
     2161 atgcactctt ggccaggcat cgggttttgg cgcttcgtcg gtgtgacagc caataattgc
     2221 tgccagtgca aagcagaaaa ttaccgttcg gaataatgac atcttgtaaa gatcgctatt
     2281 aaattaggat tgcagatcaa aatggcagat cgtcttgtat gtcggactgc ggatgttgat
     2341 gattcatatc gtcatccgga tgatcaatgc caccggtgtt tgcagggcgt ggtcctaata
     2401 aggtgaaatt atcggcgaca atttcagtga tatatttttt atgaccttcc ttgtcttccc
     2461 aactgcgatt tttaattcgc ccttcgatat aaactttcgc gccttttcgc aaaatctttc
     2521 ctgcaatttc tgcaagcttc ccccacatgg caatattatg ccattcggtt tgttctactt
     2581 tattgccatt gcggtcgcgg aaaacttctg tggttgcgag tgtgaaattc gccactacat
     2641 tgccgccttc gaaattgcgc aactccggat ctttccccag gttgcctatc aatatcactt
     2701 tgtttacacc actcatgatg ttctgtttac gtatctgtta catttaattt cgtttcaaaa
     2761 ataacgttgg ggacaggaat agaaaaacac tgagggatgg agcatatttc caatattaaa
     2821 agtacagctt tcccttcata aaatctctta ttacccctgg aaaagcaaat ctttccattt
     2881 cctttctgtt caccaattct tgcatgccat cgatttcggg agtggttttt atttctcctg
     2941 taaaaaatcg gcatatcaat cgccggtggg tcagttgttg tttgaaagtt cctgcttctt
     3001 cccattgcac cttccccagc ttccaatctt tataaacgtt tgaacgctga atatcttgta
     3061 aagttgcctc ttctgctgtc tctataagtg gaaattgcca caatgccttc cagatatccg
     3121 ctgcatgtcg gggcatgatc agcaccttgt tacccgattt catcacaaaa aagttaaaat
     3181 atctcgtttt tgcggcccgt ttcttttctt tttgggggag gatggcggtc atctcgtggt
     3241 tgaaggcata acaggaacgc cggaaggggc agtttccgca atcaggattc cgcggtgtgc
     3301 acaccagcga gccgaagtcc atcagtgcct ggttgtaagt atgtggtaat tgcttatcga
     3361 ggagcctgtc agcaagcgct tccaccattt tttttcctgc aggtgtattc acggggatgg
     3421 tgattccctg atacctggag agtatgcgta gcacatttcc gtcagttacg gcatagggtt
     3481 tgtggtatgc ataggcgagg atggcattag ccgtgtattt tcctatgccc ttgaggcttt
     3541 gaagtccttc cggggtgtcg gggaaaacgc cttttaattg ttgggtgatg acgttggcgg
     3601 tagcatgcat gtttctggcg cgggagtagt atcccaggcc ttcccagcgt ttcattacct
     3661 cattggccga cgcttttgca aggtgctgaa cggtagggta gtgtttaatg aagtcgagat
     3721 agtaggctgt cccttgttcc acgcgggtct gctgcaaaat cacctcactc aaccacacct
     3781 tgtaggggtc agtttcccca acccagggca tattgcgctt gttgaggcga taccattgaa
     3841 ggagcgtttt ggagaaagac gacatgcggg tgcaataata caaagggaaa aaaatgtgtg
     3901 gttattttat aataattaag cgaactttgc agtttaacag attgattatc aaaccaaaag
     3961 aaatatacct atgagaaagg ctgatattgt aaaccgcatt accgacaaga caggtttgga
     4021 gaaggtggat gtaatggttg tggtggaagc attttgtaaa gagatcaaag aatccatggc
     4081 tgaagggcac aacgtgtatc tgcgtggttt cggcagtttt gtggtgaaga cacgtaagaa
     4141 aaagatcggc cgtatcatca aaaagaatat tgctatagag atacccgaac aaaaaatccc
     4201 ctctttcaag ccggctaagt tgtttttaaa ccgcgtaaaa gacggtaaaa agagtaaatc
     4261 aggtctctaa gtgaaaaagt atcttattcc tgccgggatc gccgtggtgc tgattctggt
     4321 gattttcttc gcatttgatc ataagcctcc taaggtttct gttttcgacg aacgtcgtga
     4381 aaagctcatt gattcattgc aatatcccaa ttctgaggat ttgatgcaca tgcatcgcga
     4441 tatgttgcat gccggtggtg gaacgaaggc ggcgttgtta cagcaattgg cagataagtg
     4501 gatgcagctg gcccagcctt cgatagcggc agattacatc agacaacttg ccgatgcgga
     4561 tccatcttac gaacattaca tgcaagccgg cgctgcaatg ggctcgctga ttgattttga
     4621 ggctgaagaa gacctgcgta tgagccttgt ttacggggcg cgatatagtt atgagatggc
     4681 agaaaagctt cagcccggtg atttggatgc acgtatcgga ctggcatcgg tgctggtatc
     4741 gggtggtagc acgccaatgg aaggtattat gatgctgcgg gaaatggacg ccgagaaccc
     4801 cgataacatc agaattaacc tcgaattggg caaattttcg gtcatgagcg gacagtttga
     4861 taaggcatta gagagatttg atcgtgtttt acaaaaagat tccctaaatt tgcaggctcg
     4921 ttacatgagg gctcagtcgt ttcttggaat tcaggacacc gcaaaggccg taatggagct
     4981 agaaacactg aaatcgcaaa ccaccgaccc gaccctgatc gaacaggtgg ataatgagat
     5041 caaaaactta aatcattaaa aaatatttgt tatgccttcc ggtaagaaac gtaaaagaca
     5101 taaaatggct actcacaagc gcaaaaagcg tttgaggaag aaccgccaca agaaaaagta
     5161 atccaaccgg ctttttccgt acctgtatat tctcctacct tttccagccc caacaggggt
     5221 aataacttta taacatccca taaagggagc aaacccgtat ttgaacgtgg agagggaact
     5281 tattattaat accaccccca cgggtgccga gattgcatta ctcgaagaca agcagttggt
     5341 agagatacat cgcgactcgt ttagcagccg ttttaatgtt ggggatctat atctcggtcg
     5401 tgtgaaaaaa atcacgccgg gactcaatgc ctgttttgtc gatgttggtt tcgaacgcga
     5461 tgcctttttg cattataccg atctcaaccc gcaattcaag tctatctata aatacaccaa
     5521 agctgctcag gatggccggg cgtatagcat ggacaagttc cagcgcgagc ctgatatcgt
     5581 gaaaacgggg aagatcaata atgtgatctc caagagtcag catattctgg tgcaaattat
     5641 caaagagcct attcaaacca aaggtccgcg tttgagctgt gagatttctc tgccgggaag
     5701 atatttggtg ctgacgccct ttaatgaagt agttggcgtt tcgcgaaaaa ttgccagcgg
     5761 agaagaacgc aaacgcttgc aaaaagcaat tgaacaactg cgaccaaaag gttttggtgt
     5821 catcattaga actgttgccg aaaatgtacc cgttcaaaag ctgcacgacg atttaaataa
     5881 tctcgtcaat cagtggaaag agatcaccaa tcgcttacag agtgctcagg ctccgcagtt
     5941 aattttcagc gaatcgaaaa aaacgaatac gcttctgcga gatgtactga atgattcttt
     6001 taatcgcgta gtagtaaatg ataatgcgat tcaaaccgag gtaaaagatt atattcgacg
     6061 cattagtccc gggtccgaaa aaatggtgtt gatgcacagc ggaccaaaaa gtgtgtttga
     6121 acaatttggc gttacacgtc agataaaggg attgtttggt cgcacggtga atatggatag
     6181 cggtgcatat ttaattattg agcatacgga agcattgcat gtgattgacg tgaatagcgg
     6241 aaataaatct gctgttaaag gcgatcagga agcgaacgca gtttcagtga atatggaggc
     6301 tgccagagag attgctcgtc agttgcgatt gagagatctc ggcggaatta ttattgtcga
     6361 ttttatcgac atgaaaaatc cggaaaataa aaaggctgtt tacaatgcat tgaaaaatgc
     6421 gatggcgggg gatagggcga agcacactat cttaccgatt tcgaaattcg gtgttgcaca
     6481 aattacacgc cagcgtgtgc gtccggaggt gaatatttct actgctgaag tttgtcctac
     6541 atgtaatgga acgggtaaaa ttgaagcgag tattttattg ctggaagata ttgagcgcaa
     6601 gctgaaacat cttgttacca tacaaaatct gaaagcggtg aatcttatgg tgcatcctta
     6661 tgtggctgca tatctgaaaa aaggttggtt cttccgcagt ttatattggc agtggcgacg
     6721 tacttacaaa gtaaagctta aggttacatc gagtgacgat tttcattata tggaatatcg
     6781 cttcttcgat ttgaatgaag gggagataaa cgtggagtag gagagaggag agaggagaga
     6841 ggagaaagga gagaggagag agtgggtgag cttgcctcgc cgagtaggcg ggtgggtgag
     6901 cgggtcagtg ggtgagtggt gagtggtgag ttgtgagttg tgagtggatt gatacttgat
     6961 acttgatacc tgatacctga tacttgatac ctgatacttg atacctgata cctgatactt
     7021 gatacctgat accaaattcc agatactaga tattcaaatc atcacgaaag atacttccct
     7081 tcaataggca ttcttcttcc tgttccgaag gctttggggg atacgcggag tatgggtggg
     7141 aattgccagc gtttccattc attgctgttc accattttta aggtgcgatt gacgatggca
     7201 gggttgaaac ctttttctat gatgtcttgc ggactctgat gtaattcgat gtattgatac
     7261 aaaatagcat caagaatatc ataaggtgga agcgtatcct gatctttttg tccgggtttt
     7321 aattctgcgc ttggtgcttt ggtgatggag tgcaacggaa taatttcttc gttgcgatta
     7381 atataatttg ataaggtata gacttgtgtt ttatatacat cggcgagtac tgctaggcca
     7441 ccgcagagat ctccgtagag tgtgccgtaa ccgacagcag cttcgctttt atttgttgta
     7501 ttcaataaaa tgttaccgtg tttattacac atcgccatga gtgtgagtcc gcgaagtcgt
     7561 gcctgtagat tttcttctgt tacgtcttcg ggtttattgt tgaagagtgg ttgtaattgc
     7621 gattcaacgg cgttgaacat atttccgata ggcacaatat caaaacgcat accgagattt
     7681 tttgcaaggt cttcggcgtc tttaatagag tgatcggtgg aatattgtga gggcattaat
     7741 aatccccaaa cattttcttt tcctaatgcg tgtactgcca gaacagctgt aagtgctgaa
     7801 tctattccgc cacttaatcc aagtatagct tttttgaatc ccagtttttc gaaataattt
     7861 ctgatgcctg taacaagtgc ttgataaatt agggcagtgg tatctttttc ttgaacgata
     7921 tcggtgttga gtgtttttga tgacgcaagc acatcatcca ggtcgtatac ttttatacat
     7981 tctgtaaagt atggcatttc gtcgtataca acagcgttgg cattgataac cagactaccg
     8041 ccatcgaaga tgaggtcggt ttgtgcgccg caatgattta cataaaatac ggggatgcc
//
LOCUS       DAOQHY010000140         8076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_4582_length_8076_cov_6.191871, whole genome shotgun sequence.
ACCESSION   DAOQHY010000140 DAOQHY010000000
VERSION     DAOQHY010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8076
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4582_length_8076_cov_6.191871"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="PLL28_11065"
     CDS             <1..823
                     /locus_tag="PLL28_11065"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HNF69909.1"
                     /translation="ATIALYQYWYKDYPLNHFHLFNDNGEWLQYDKMGHFYTAYFETN
                     LTTEFYQWTGMSDNAAYWSAAATGSILQLTIEVFDGFSEKWGFSVGDFAANTFGATLC
                     TAQNMLWDEQRIKVKFSSHFTDYSGYDQPVQDRAAQLFGTTGPEKVLKDYNGQTTWLS
                     VNPSMFMSPETRFPKWLMISGGYSVDGLLGGYENKWCDDPEMKPEDCPPDQLTDYSQI
                     PRERQFYLSLDVDLTQIPTNSPYLKMVFNVLNIVKFPAPALQLQNGNLSFKPIYF"
     gene            complement(841..1494)
                     /locus_tag="PLL28_11070"
     CDS             complement(841..1494)
                     /locus_tag="PLL28_11070"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNF69910.1"
                     /translation="MKRTIKWNIAQKAELKWWQQYLKGKDVEAYHTWKRAYWQGILDK
                     IAGSAPIREGMSILDAGCGPAGMFMQLPNCNVDALDPLLDEYEKNLPHFKRSMYANTT
                     FHALPLEQYQTDKQYDIVFCMNAINHVSDIAFSYDLLAKMVKPGGKLVVSIDAHNHNF
                     FKHLFRMIPGDILHPHQYDLKEYSAFITDRNCRILQTEKLKHEFFFDHYVQVAQKIS"
     gene            1531..2499
                     /locus_tag="PLL28_11075"
     CDS             1531..2499
                     /locus_tag="PLL28_11075"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNF69911.1"
                     /translation="MQIPVGEQVVILDSGIIGVIEYYNGSTAYINVNGEIHQAHKKDI
                     ASVSEFGGKGKSTIKKEELTQAAKDIGLFIHWRAVKSMGGDVDRFEVELVNNTPLRLL
                     FEYKWYLLEELQTTVRNTITPNATIRLHGMKPDAINDRPVSTLQCWRQDDKGITISVA
                     DSEVKLRPKQYVTKLSTEPYLSSGIIVFPVNVSDSSLQIPASEKKPTPAFNFNEKKKV
                     PVINEVLQKAAMPDYIDLHVEKLLPSHKHLSSTEILAMQISQFRHFLEKAIRLKLHRI
                     YAIHGHGKGVLRTEIEKTLKKYPEVTSYNNDYNPRFGHGATEIILD"
     gene            2578..2966
                     /gene="rnpB"
                     /locus_tag="PLL28_11080"
     ncRNA           2578..2966
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLL28_11080"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2987..3208
                     /locus_tag="PLL28_11085"
     CDS             2987..3208
                     /locus_tag="PLL28_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69912.1"
                     /translation="METLKNVILCPFCGHLSQMLWVHGHGQCAICGMTIDECCRGEQA
                     CEIEDPQKRKAKKKITPQKEEPNNETTKP"
     gene            3189..3998
                     /locus_tag="PLL28_11090"
     CDS             3189..3998
                     /locus_tag="PLL28_11090"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HNF69913.1"
                     /translation="MKLLNLKSALLMLACCLGIQFASAQNLVTFAPPEPPKPAVPVNI
                     DTVITTIDAAKGYKRITITANNIVVSDGYMIGNKKTGTWSTFQLNGSLLTLAEYDNDV
                     KSGNYLEFDKNGNLLVLEGYKNGKLHGDQKKYTQGKTGRVLKSSYGYADGLYHGDCIE
                     YTVDGLIQSSMHYANGKKDGTSRWYFSSGKVAMEQHYTNDQLNGKQLIYDQTGALISE
                     GTYTNNMKSGTWNENYPNGKLKSTGQYTNDKKTGEWKYYKEDGSLEKTEKF"
     gene            4139..5494
                     /locus_tag="PLL28_11095"
     CDS             4139..5494
                     /locus_tag="PLL28_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNF69914.1"
                     /translation="MASLLLIDDEKSIRKTLKEILEYEGYTVDEAGDGAEGLRMIQDA
                     DYDIVLCDIKMPKMDGMEVLEKAQITNPDVPFIMISGHGTLETAVEAVKKGAFDYVAK
                     PPDLNRLLITIRNALDKSNLITETKVLKRKVSKTREIIGDSPAIMKIKETIDRVAPTD
                     ARVLITGDNGTGKELVARWIHERSNRASGPLVEVNCAAIPGELIESELFGHEKGAFTS
                     AIKQRIGKFEQANGGTLFLDEIGDMSHNAQAKVLRALQENKITRVGGDKEISVDVRVV
                     AATNKDLLKEVESNNFRLDLYHRLSVILIHVPSLNDRKDDIPLLAERFVKEICEDYGV
                     PKKVVTPKAMIEIQNINFTGNIRELRNVIERLVILSDQKITDEDVLNYAQSPKSGGSP
                     KDLYERFEKFQDFKDFVEKEFILHRLNKNNWNVSKTAEELDIQRSHLYNKIEKYNLKR
                     D"
     gene            5506..5682
                     /locus_tag="PLL28_11100"
     CDS             5506..5682
                     /locus_tag="PLL28_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69915.1"
                     /translation="MAANKPKAQSKTDQMPKDKPMANESQENIVTNAANMLIGIGSVV
                     IMALIIIWMIKLLG"
     gene            complement(5776..6711)
                     /locus_tag="PLL28_11105"
     CDS             complement(5776..6711)
                     /locus_tag="PLL28_11105"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNF69916.1"
                     /translation="MSFAVTVIGSNSAIPAHGRHPSAQVVTINDRLYLIDCGEGTQMR
                     FNELGIKWMKINQIFISHLHGDHFFGLIGVLSTYHLMRRNRPLDVYAPAPLADIIKMQ
                     LEAGESQLNYELRFHELDTVNTGTIYENEDITVDTIVLNHRIPCVGFVFREKQRDRKV
                     IREKLQEFQIPVELIPDIKKGMDILDEKTGNRILNKDITIDPPLPRTYAYCCDTAYYP
                     AMVPFIANADLVYHDCTFDEASSQRATDTFHSTTKQAASIALQANAKKLMIGHFSSKY
                     DDLLPLLREAQEVFSNTILAIEGKTVELERVTGTP"
     gene            complement(6716..7087)
                     /locus_tag="PLL28_11110"
     CDS             complement(6716..7087)
                     /locus_tag="PLL28_11110"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNF69917.1"
                     /translation="MKFTVDKKERYCIFKLDEPRLDSILSANLKSELVLLNQEGFRNI
                     ICDLSEVNFIDSSGLSALLIGHRICKDSNGTFVLIGCGESTIKLVKISQLDSILNIVP
                     TESEATDFILMEEIERELGNE"
     gene            7305..>8076
                     /locus_tag="PLL28_11115"
     CDS             7305..>8076
                     /locus_tag="PLL28_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNF69918.1"
                     /translation="MDAKFSPKVKEVISYSREEALRLGHDHIGTEHLLLGLIRDGDGL
                     AIKVLLSLDVDIAMLRKTVEDAIRDKASRQPFNAGSLPLTKQAEKVLKITVLEAKVLK
                     SDTIGTEHLMLSILKNKDNVATQILGQFDVDYDVFRQELDYVRQDQGQREIPPTNQNE
                     DENFEEEEKGDRGFNVPRKPGAAKSKTPVLDNFGRDVTRAAEEGRLDPIVGRQEEIER
                     VSQILSRRKKNNPILIGEPGVGKTAIVEGLALRIIQRKV"
BASE COUNT         2560 a         1691 c         1684 g         2141 t
ORIGIN      
        1 tgctacaata gcactttatc aatactggta taaagattac ccgcttaatc attttcacct
       61 ctttaacgac aacggcgaat ggttgcaata tgataaaatg ggtcacttct acaccgctta
      121 tttcgaaaca aatttgacta cggaatttta tcagtggaca ggtatgtccg acaatgctgc
      181 ttattggtct gcagcagcaa caggttccat acttcaactt accattgaag tgttcgatgg
      241 attttccgaa aaatggggat tttcagtcgg cgactttgct gcaaatacct tcggagcaac
      301 cctttgcact gcacaaaata tgttgtggga tgaacaacgt ataaaagtga aattttcctc
      361 gcactttact gattattccg gttatgatca accggtgcaa gacagagcag cgcaattatt
      421 tggaactaca ggaccggaaa aagtgctcaa agattacaat ggtcaaacaa cgtggctaag
      481 cgttaaccct tcaatgttta tgtcgcctga aacacgcttt cctaaatggt tgatgatatc
      541 cggaggttat agtgttgatg gcctgctggg aggttatgaa aataaatggt gcgacgaccc
      601 tgaaatgaaa cctgaagatt gcccccctga tcaattaact gattactctc aaattccgag
      661 agaacgccaa ttttatcttt ccttggatgt ggacctcacg caaatcccga caaattctcc
      721 ttacctgaaa atggtattca atgtgttgaa tatcgtcaaa ttccccgcac cggcattgca
      781 attacaaaac ggcaatctca gctttaaacc aatttatttc tgataaaagg gaattaacct
      841 ttaagaaatt ttttgcgcaa cctgaacgta gtggtcgaag aaaaattcat gcttgagttt
      901 ttccgtttgc agaatcctgc aatttcgatc agtgataaat gcactgtatt ctttcaaatc
      961 atattgatgc ggatgcaaaa tatcgccggg tatcatgcga aataaatgct tgaagaaatt
     1021 atgattgtgt gcatcaatgc taaccaccaa cttgccaccg ggttttacca tttttgccag
     1081 cagatcataa ctaaatgcaa tgtccgaaac gtgattgatg gcattcatgc aaaacacaat
     1141 atcatattgt ttatctgtct gatattgctc tagaggcaaa gcatgaaaag tggtattggc
     1201 atacatactg cgcttgaaat gaggtagatt tttttcatac tcatccagca gtggatctag
     1261 tgcatcaaca ttacaattgg gtaattgcat gaacatcccg gcaggaccac accctgcatc
     1321 taaaatgctc atgccttcgc gtataggcgc agagcctgca attttgtcta aaattccctg
     1381 ccaataagcg cgtttccagg tatgataagc ctcaacatcc ttgcctttca gatactgttg
     1441 ccaccacttt agctcagcct tttgtgcgat attccacttt atcgttcgtt tcatgtttac
     1501 ctttgcaacg gcttcaaagg taagtaaatc atgcagatac ctgttggtga acaagtggta
     1561 attctcgatt caggcattat tggtgttatt gaatactata atggcagcac agcctatatc
     1621 aacgtaaatg gcgaaatcca tcaggcacat aaaaaggata ttgcctcagt cagcgaattt
     1681 ggtggaaagg gaaaatctac tatcaaaaaa gaggagctta cccaggctgc aaaagacata
     1741 ggtttattca ttcattggcg tgctgtaaaa agcatgggtg gcgatgtcga cagatttgaa
     1801 gttgaacttg tcaataacac acccttgcga ttgttatttg agtataaatg gtatttactc
     1861 gaagaattgc aaacaactgt caggaataca ataaccccca atgccaccat caggcttcat
     1921 ggtatgaaac ccgatgctat caatgatcgt ccggtatcta ctttgcaatg ctggcgtcag
     1981 gatgataaag gaattacaat ctcagttgca gatagtgagg tcaaactacg ccctaagcag
     2041 tatgttacca aattatcaac tgaaccgtac ctttcatcag gtataattgt tttcccggtt
     2101 aatgtgtcag acagtagtct tcaaattcct gctagcgaaa agaaaccaac acctgcattt
     2161 aatttcaacg aaaagaaaaa agtcccggta ataaacgagg tattacagaa ggcagcaatg
     2221 ccggactata ttgatctgca tgtagaaaaa cttttgccat cacataagca tctgtcctcc
     2281 actgaaattc tggctatgca gattagtcaa ttcaggcatt ttctagaaaa ggctattcgt
     2341 ttgaaattgc atcgcattta tgccatccat ggtcatggga aaggtgtatt gcgcaccgaa
     2401 atagagaaaa cattgaagaa atatcctgaa gtgacatcat acaataacga ctataacccg
     2461 cgttttggtc atggagctac agaaattatt ttagactagt ctaaattttt agatggcgtc
     2521 aaatcggcaa aatccctaat tttgagatga gttcaaataa tcgttcttaa agtaattctc
     2581 gcaaaccgtt ctatcgctgc ttaaccggtg catgcatcgg ggtagtggag gaaagtccgg
     2641 ataacacaga gcaccgcacc acctaacaga tggatatcgg cttcggtcgg taatggatag
     2701 tgccacagaa aattaccgtc ctgacaaaag tgttcgcact tttcgtcagg gtaagggtga
     2761 aaacgcgagg taagagctca cgggtgagaa tggcaacatt tttgccgggt aaaccctgcg
     2821 ggttacaatg ccaaatatgc cggagtaaaa gaagagttgc tcgctctaat cttcggtggg
     2881 taggcagcaa gatccttata gtgatgtaag gaccagataa atgatagaaa ctcaaacaga
     2941 atccggctta tgagtttgcg ggaattgttt taagaatgaa acaggtatgg aaacgttgaa
     3001 aaatgttatt ctttgcccat tttgcggaca cctcagccaa atgctgtggg tgcatggtca
     3061 tggacagtgt gccatttgcg gaatgaccat cgatgaatgt tgcagaggtg aacaagcttg
     3121 tgaaattgaa gaccctcaaa agagaaaagc aaagaaaaaa atcactcctc aaaaagaaga
     3181 acctaataat gaaactacta aaccttaagt ctgcattgtt gatgttagct tgctgcctgg
     3241 gaatacaatt tgcctcggca caaaatcttg tcacgttcgc accgcccgaa cctcctaagc
     3301 ctgcagtgcc tgtaaatatc gatacagtta ttactaccat cgatgcagca aaagggtata
     3361 aacgcattac catcaccgcg aataatattg tggtatctga tggctatatg attggcaata
     3421 aaaaaaccgg aacatggtcc acatttcaat taaatggctc tttgcttacc ttggcggaat
     3481 atgataatga cgtaaaatcc ggtaactatc ttgaatttga taaaaatggt aacctacttg
     3541 tactcgaagg atataaaaat ggtaaactgc atggcgacca aaaaaaatat acacagggaa
     3601 aaacaggaag agtgctgaaa agcagttatg gatacgctga tggattatac catggcgatt
     3661 gcatagaata tacagttgac ggtctgatac aatcgtctat gcattatgct aatggcaaaa
     3721 aagatggcac ttctcgttgg tatttttcct ccggaaaagt tgccatggaa caacattata
     3781 caaacgacca gctaaacgga aaacaactca tttacgatca aaccggggcc ttgatctctg
     3841 aaggcaccta taccaacaat atgaagtccg gaacatggaa tgaaaactat cccaacggca
     3901 aactcaaatc gaccggccaa tataccaacg ataagaaaac cggcgaatgg aaatactaca
     3961 aggaagacgg tagtctggag aagacagaaa aattctaaaa aatagtacaa acattatata
     4021 tttcctgtcc aattttctac atttgggcac gtttactttt ttggacaagg ttcacacact
     4081 tctttggaca atcaagtaaa ttgaacttaa cacgaaccaa cacaatgaat taacgaacat
     4141 ggctagttta ttattaattg acgacgaaaa gagtatcagg aaaaccttaa aggagatact
     4201 cgaatacgaa gggtatacag tagatgaagc aggcgatggt gccgaaggtc tgcgtatgat
     4261 acaggatgct gattatgata tcgttctctg cgatatcaaa atgcccaaaa tggatggtat
     4321 ggaggtgctc gaaaaagcac aaataacaaa tccggatgtg ccatttatta tgatcagcgg
     4381 tcacggaaca ctggaaacag cagtggaagc agtgaaaaaa ggtgcgtttg attatgtagc
     4441 aaaaccacct gatctcaacc gactactcat cacaatccgc aatgccctcg ataagtcgaa
     4501 tttgatcacc gaaactaagg tgctcaaacg caaggtgagc aagactcgcg aaattatcgg
     4561 cgattctcct gccatcatga aaattaagga aaccatcgat cgcgtagctc caactgatgc
     4621 acgcgtactt attacgggtg ataacggtac cggcaaagaa ttagtggcac gttggattca
     4681 tgaacgaagt aaccgtgcat caggtccttt agtggaagtg aactgcgccg caattccggg
     4741 cgaattgatt gaaagcgaat tgttcggcca cgaaaaaggg gccttcacct cagcaattaa
     4801 acaacgcata ggtaaatttg aacaagcaaa cggcggaaca ttattcctcg atgaaattgg
     4861 cgatatgagc cataatgccc aagcgaaagt attgcgtgca ttgcaggaaa ataaaattac
     4921 acgcgttggt ggcgataaag aaatatctgt agatgtacgc gttgttgccg caaccaataa
     4981 agatctgctt aaagaagtgg aaagcaataa tttccgcctc gacctctatc atcgattgag
     5041 tgtaattctc attcatgtgc cttcgcttaa cgatagaaag gatgacatcc ctctgctggc
     5101 agagcgcttc gtaaaagaaa tctgtgaaga ttatggtgtg cccaaaaaag tagttacacc
     5161 aaaggcaatg atagaaattc aaaatatcaa cttcacaggt aatatccgcg aactccgaaa
     5221 tgtgatagag cgattggtga ttttatccga tcaaaaaatt acagacgaag atgtgctgaa
     5281 ttatgcccaa tcaccaaaaa gtggtggcag cccaaaagac ctttacgaac gcttcgaaaa
     5341 atttcaggat tttaaagatt ttgttgaaaa ggaatttatc ctgcatcgct tgaataaaaa
     5401 caactggaat gtttccaaaa cagccgaaga gttggatatt caacgcagcc acctctacaa
     5461 taaaatcgaa aaatataatc tcaaacgtga ttgatctttt ttctcatggc agcgaataaa
     5521 cccaaagctc aaagcaaaac tgatcaaatg ccaaaggata aacccatggc aaacgaatct
     5581 caggaaaaca ttgtcaccaa cgctgccaat atgcttattg gtattggcag cgtggtgatc
     5641 atggcgctga taataatttg gatgatcaag ctgctaggct gaacatcttt ttctgctccg
     5701 taaaatcctg atgttgcctt gtgcacctga aatgtgcatg ggtattaatt aactgcaaaa
     5761 acaattcatc aaaactcaag gagtacctgt aactctttcc agttctactg tttttccttc
     5821 aatggcaagt atggtatttg aaaatacttc ctgcgcctcc cgaagcaatg gaagcaaatc
     5881 gtcatacttg gaagagaaat gtcctatcat caattttttt gcattcgctt gtaacgcaat
     5941 tgaagccgct tgttttgttg tagaatgaaa agtatctgtt gcacgttgcg aacttgcttc
     6001 atcaaatgtg caatcgtgat aaactaaatc ggcatttgca atgaaaggaa ccatcgccgg
     6061 ataataggct gtatcgcagc agtaagcgta agttcttgga agaggagggt ctatagtaat
     6121 gtctttattc aggatgcgat tacctgtttt ctcatccaat atgtccattc cttttttgat
     6181 atcaggtatc aattcaacag ggatctgaaa ttcttgtaat ttttccctaa tcacctttcg
     6241 atcgcgctgt ttttcccgaa atacaaatcc aacacaagga atgcgatgat tcagcacaat
     6301 ggtatctacc gtaatatcct cattttcata aatcgttccg gtatttacag tatccaattc
     6361 atgaaatcga agttcataat taagctgact ttcacccgcc tcaagttgca ttttgatgat
     6421 gtcggcaagc ggtgcaggtg cataaacatc aagtggtcga tttctgcgca tcaaatgata
     6481 ggtcgacaaa acaccgataa gtccgaagaa atgatcgcca tggaggtgcg aaatgaaaat
     6541 ctgattgatc ttcatccact tgatacccag ctcattaaag cgcatctggg tgccctcgcc
     6601 acagtcgatt agatataaac ggtcgttgat ggtcaccacc tgagcacttg gatgcctgcc
     6661 atgagcagga atagcagaat tactcccaat tactgtgacc gcgaatgaca tacccttatt
     6721 cgttgcctaa ctctcgctca atctcttcca taaggatgaa atcggtagcc tcggattcgg
     6781 taggtacaat gttcaatata ctgtcaagct gcgaaatctt caccaacttg atagttgact
     6841 cgccacagcc aataagcaca aaggtgccat tgctgtcctt acagatgcga tgccctatca
     6901 gcaaggcgct caagccggaa ctgtcgatga aattcacctc gctgaggtca catataatgt
     6961 tgcggaatcc ttcctgattc agcaaaacca gctcactttt caaatttgca gataagatcg
     7021 aatccaaacg cggctcatcc agcttaaata tgcagtaacg ctcctttttg tctactgtaa
     7081 acttcatacg ctcagttgtg gtgtaaaaat aaactttacc ccgcgaaggt aggtaattaa
     7141 aattttttcc aaatttgtta gtctaagtga aagggcccta tggaattttt taggctcctg
     7201 aatgtttaca tagataagac attgtcgaaa taacaatttc ggccccgaat ttgttaattt
     7261 gtcaggtaac gcagagttgg taaaattctt gaacaataat cagaatggac gcaaaatttt
     7321 ctcctaaagt aaaagaagtg atctcctaca gccgcgagga agccctccgc ctgggtcacg
     7381 accacatcgg aaccgagcac ctcctcctcg gccttattcg cgatggcgac gggctggcga
     7441 tcaaagtgct gctcagtctc gacgtggata ttgccatgct tagaaagacc gtagaggacg
     7501 ctatacgcga taaagcctct cgtcagcctt tcaacgccgg cagccttccc ctcactaaac
     7561 aagccgaaaa agtgctcaaa ataaccgtcc ttgaggctaa agtccttaag tccgatacca
     7621 taggcaccga acaccttatg ctgtctatcc tcaaaaacaa ggataatgtg gcaacacaga
     7681 ttttaggaca atttgacgtg gactatgacg tatttcgtca ggaattggat tatgtgcgcc
     7741 aggatcaggg tcaacgtgaa attcctccaa caaaccaaaa tgaagatgaa aacttcgaag
     7801 aggaggaaaa gggcgaccgt ggattcaatg ttccacgcaa accgggtgcg gcgaaatcga
     7861 aaacgcctgt gcttgacaat ttcggtcgcg atgtaactcg cgcagctgaa gaaggtcgtc
     7921 tggatcctat tgtcggtcgc caggaagaaa ttgaacgcgt ttcacaaatt ctcagccgca
     7981 ggaagaaaaa taacccaatc cttatcggag aaccgggtgt tggtaaaaca gcaattgttg
     8041 aaggtctcgc actgcgcatt attcaacgca aagtgt
//
LOCUS       DAOQHY010000141         8015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_4634_length_8015_cov_3.933668, whole genome shotgun sequence.
ACCESSION   DAOQHY010000141 DAOQHY010000000
VERSION     DAOQHY010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8015
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4634_length_8015_cov_3.933668"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1763)
                     /locus_tag="PLL28_11120"
     CDS             complement(<1..1763)
                     /locus_tag="PLL28_11120"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HNF69919.1"
                     /translation="MTATLSPLYQSQSLKDNMQHIIPTYEALCDLHLLETARKIVTNK
                     ATAGGVDRKSAKSFTDQDLKKLHTELVQHKYVPEPHLAVKIPKGENAYRHLGLLTVRD
                     KILQTAILLQIEPLIDQTFYPSSFAYRKGLGPVDASRKVFALIKSNQFSWAAKMDIKN
                     FFDSVDHTLLATQLQKHISDPELFNLIMLCLKMGTVDWNNQWQDRLLGIPQGSILSPL
                     MANLYLTELDRAIADEGAAYVRYADDFIVLTKNEKSAANIIGIVKQFVAEALHLELNE
                     KSYVAPLRHGVEFLGIRFYNNHYTLASDKINSLKHKIDQAIEVDKGINGRKLRDVLEG
                     IHRYYARMVHEKVLLPIDAHLLESIESFCTANKTAFSSALQLHKMLEGVWFITNTYKE
                     KRHAEARRIVSALFQKGSAVLPEQIRIDQQSLIEAKKRAYEKLERRGFELLIHKSGVF
                     LGKTYHHFTVKEKGELLFKAPLANVKHISILGEGVSVSGYALCYCAENNIPIDFYVTH
                     GQPVARVYSMHTHDSDLLMKQLQALTNGKGHHIAYQLVVAKIKNQLNTIKYLTKNDVL
                     DNACASFTEPLDVILQELDQI"
     repeat_region   1805..2761
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1805..1839
                     /rpt_unit_seq="gtttctaaaggctaactcgcctactcatcggatac"
     gene            complement(3170..4075)
                     /gene="cas6"
                     /locus_tag="PLL28_11125"
     CDS             complement(3170..4075)
                     /gene="cas6"
                     /locus_tag="PLL28_11125"
                     /inference="COORDINATES: protein motif:HMM:NF021551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR system precrRNA processing
                     endoribonuclease RAMP protein Cas6"
                     /protein_id="HNF69920.1"
                     /translation="MRALEDGFISGFMGHTLHGALGHSIRRALCNDYRPGNQTECSTC
                     NCLYQPWYNPAQSPDPMVAKRYKDPPRPYFIGCNFDWEKRRVSYDDVLEWTLHVAGNS
                     PGDMNQIVEIVEALCANGLGSGELKWELDIVLAHETTLLLDWGYTDNNFQHITIDFKT
                     PFWTNDKQMQESGTITFDMLLRYAIERIRAMALYYGNAVLPQEEFIQETLDLAQGAGI
                     VEQRLRKWSIDRDRGYPLKGWTGRIRYDRVPNTAINVLNAAQHLHLGRNTTFGCGMIE
                     IQPFFKKDDNTVRRRKPRKLPPVDE"
     gene            complement(4152..4826)
                     /locus_tag="PLL28_11130"
     CDS             complement(4152..4826)
                     /locus_tag="PLL28_11130"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HNF69921.1"
                     /translation="MPDKNRFIAEAAGVIIKHPLLSLVDINGEPALIGTITLIDQKEN
                     PIDNYQIKILCGQFPHKFPLVFETGGKIPKNIDWHMYEQTGNCCLCIPPEERKLCNMG
                     FSLVSFMDDFVLPYFFHQTYRRKNGFFRDEWQHGIAGLYAYYSDLFATTNIFEIIRLL
                     EKIVAGGPPHRNSPCLCGSGQKYRHCHKERVKSVFIENRAEITSDISLLKQFAVMAAL
                     AMPIIA"
     gene            complement(4816..5583)
                     /locus_tag="PLL28_11135"
     CDS             complement(4816..5583)
                     /locus_tag="PLL28_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69922.1"
                     /translation="MKTGIRTKDDICDVDDGIYFFRKPDVTPNTLQAWVLQAVTGHTE
                     TPPEHREKCIRLIFAGDYELDMPVYYSEDKLSYYLATNTNGWGADDPKEMVDWFVAQK
                     DEAGHLIKIVKYLKAWCDYKRNKMPNGLAMTILAGNAKSKIVYNDRDDINLRDTLKEI
                     QKTLLASFTCIVPATPNDNLFAEYDDTRKKNFLNALDAFLVDADAAIRESNFLNASKL
                     WRKHLGDRFPLGEDKIDPDLSNPALIAGIKSSKPYAG"
     gene            complement(5935..7104)
                     /locus_tag="PLL28_11140"
     CDS             complement(5935..7104)
                     /locus_tag="PLL28_11140"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNF69923.1"
                     /translation="MSELARQYPHIAFRRHWELTADAWRIIGECKAYAEAITNTPILP
                     HYYDELMKVSLLKGAQSTTAIEGNTLSDEEIERVIAGEDLPPSQGYQQREVKNVVDAL
                     NSILFDVLDGQANKLISKELILTFHQLIGKNLGETFAAIPGKFRNNEVIVNRYRCPDH
                     RDVEPLIDKLCAWMQDEFAFGKKRQAFHNVLIQAIVCHVYLEWIHPFSDGNGRTGRLL
                     EFYILIRGGNPDIASHILSNYYNQTRPQYYYYLDKAAKDESLTSFIEYALVGYRDGLQ
                     GKLKEIQNSQFENTWQRHVYDTFDNLKNTKRNVVFRRQRRLALNLPLKKEFSAEEVGE
                     ISIPLAKLYGGISLKTIQRDLNKLEQIELIKREGKRYHTNTLNISRMMAKQNKII"
     gene            7391..7789
                     /locus_tag="PLL28_11145"
     CDS             7391..7789
                     /locus_tag="PLL28_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69924.1"
                     /translation="MADHKAKHLEFIQEAIKRMNTLSFQIKGWCITLVSAINVLAAKD
                     VDHDHILLVYWIVPIFWAMDAFYLSQERQYRTLYNEVRLKKEADIDFNMQTQASILGK
                     NSWISAMFSVSNWPVYLILLGVTVYIMFNC"
     gene            7792..>8015
                     /locus_tag="PLL28_11150"
     CDS             7792..>8015
                     /locus_tag="PLL28_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69925.1"
                     /translation="MISDPHVIELCKRLLDQWEGDDIPYENRKGYNIGALIVSPENKV
                     VAFERNAVNVNANVTEHAELNVIRNYLRKSG"
BASE COUNT         2285 a         1617 c         1562 g         2551 t
ORIGIN      
        1 tttatttgat cgagttcttg tagaataaca tctaatggct ctgtaaatga cgcacaagca
       61 ttatccagta cgtcgttttt agttaaatat tttatcgtgt tcaattggtt cttaattttt
      121 gctaccacca attggtatgc gatatggtga ccttttccat ttgtaagtgc ctggagttgc
      181 ttcatcagta aatcagagtc atgtgtatgc atgctataca cacgtgcaac gggttgacca
      241 tgtgtaacat aaaaatcgat tgggatatta ttttctgcac aataacagag tgcatatccg
      301 gaaacagaaa cgccttctcc cagaatactg atgtgtttta catttgccaa aggcgccttg
      361 aaaagaagct ctcctttttc ttttacggta aaatgatgat atgttttgcc aagaaacacg
      421 ccggatttat gaataagtaa ttcaaaaccc ctgcgctcta acttttcata cgctcttttt
      481 ttagcttcta ttaaggattg ttgatctatg cgtatttgct caggcagaac tgcagaacct
      541 ttttggaata gtgcacttac tatgcgccga gcctcagcgt gtcttttttc tttataggta
      601 tttgtaatga accaaacccc ttccaacatt ttatgcagct ggagtgcaga tgaaaatgcg
      661 gttttgtttg cagtacaaaa agattcaata ctttccagca agtgcgcatc aattggaagc
      721 aacacttttt cgtgaaccat ccttgcataa tacctgtgta taccttccag tacatcgcgt
      781 agttttctgc cgtttatccc tttatctacc tcaattgcct gatcaatctt atgctttaag
      841 gaattaattt tatcagaagc caatgtgtaa tggttattat aaaagcgaat gcccaagaac
      901 tcaacaccat gccgtaaagg agctacgtat gatttttcat ttaactcgag gtgtaatgct
      961 tcggcaacaa attgttttac aatgccaatg atattagccg cggacttttc atttttggta
     1021 agcacaataa aatcatcggc atagcgaaca taggcagcgc cttcatctgc aatagcccta
     1081 tccagttctg tgagatacag attcgccatc aatggagata aaatgcttcc ttgagggatg
     1141 cccagtaaac gatcctgcca ttgattattc cagtctaccg tgcccatttt taaacataac
     1201 atgatcaggt tgaataattc gggatcgcta atgtgttttt gcaactgtgt cgctaatagg
     1261 gtgtgatcta cactatcaaa aaagttttta atatccatct tagcagccca gctaaattgg
     1321 ttgcttttga taagcgcgaa cacttttctt gaggcgtcta caggcccaag ccctttgcga
     1381 taggcaaaac ttgagggata gaatgtttga tcaatgagcg gttctatttg aagcagaata
     1441 gctgtttgta aaattttgtc tctaacagtt aacaaaccaa ggtgtctgta cgcgttttct
     1501 ccttttggaa tctttacagc cagatggggc tctggcacat acttatgttg cacgagctct
     1561 gtatgtagct ttttgaggtc ctgatcagta aatgacttag ccgattttct atcgactccg
     1621 ccggcagtcg ctttatttgt tacaattttt cttgctgttt caagtagatg caaatcgcaa
     1681 agcgcctcgt atgttggaat gatgtgttgc atgttatctt ttagagattg tgattgatac
     1741 aaaggactta aggtcgcagt cacattgctt tcgtgtgttt gactatcgaa attaagttcg
     1801 gcttgtttct aaaggctaac tcgcctactc atcggatacg cgacgggata aaaaactgtc
     1861 aagcttggta cttcgtttct aaaggctaac tcgcctactc atcggatacc gcgtatgaga
     1921 aacacttggt tacccaaaag tatcgtttct aaaggctaac tcgcctactc atcggataca
     1981 aaaaattatt gaaggaaaag accctttaga tatgatcagt ttctaaaggc tatctcgcct
     2041 actcatcgga tacttgtatt tgttttccgt tttatgatgg tcagatttag tttctaaagg
     2101 ctaactcgcc tactcatcgg atacaattac ctggattttg tttgtccgta gaacattaag
     2161 tttctaaagg ctaactcgcc tactcatcgg atacacctga atgtaaaaga agtcgctttt
     2221 aaatggcgaa gtttctaaag gctaactcgc ctactcatcg gatactatca aaagataaat
     2281 gctcacatgt aatcttcaaa gtttctaaag gctaactcgc ctactcatcg gatactcaat
     2341 tatggcaagt aaaacccaac gtggcagaag tgtttctaaa ggctaactcg cctactcatc
     2401 ggatacctat tgttcctaac aaataaattg atgacgctat ggagtttcta aaggctaact
     2461 cgcctactca tcggatactc tttctcatta tataaatatt tttgttgcac tgtagtgttt
     2521 ctaaaggcta actcgcctac tcatcggata ctgaataaat tatgagtaac accactaatc
     2581 cacacaaagt ttctaaaggc taactcgcct actcatcgga tacagaagaa gatgagcacc
     2641 agcaatacct ccgaaatgtt tctaaaggct aactcgccta ctcatcggat acaaaaaccc
     2701 tagcactgtt cgaaggcaac taaaccgttt ctaaaggcta actcgcctac tcatcggata
     2761 cacactgata atcaagcact tataaaggat gttccacaca caacccgtga aacatgcgcc
     2821 accgctcaat gctatagcca tttcaaaact actcaaatcc ctttgtaatt tacaataagt
     2881 gaatgggttc tgccacatag attttatata aagtattgaa attcagctat ttaggttgta
     2941 aaaatttttc tcatgggtag ggaaatttta cgatgatttt ttggtttgag aaaaaacgcg
     3001 gttttaacag tttataaagc attgataatc aaagatttat tttggcacat gaaaaaaata
     3061 ttctcaatcc tacccggggt gtccaatacc agaaggctcg tgagtaaatt ttaagcttaa
     3121 aaaaaatgat ttttagttaa aatagcctgt tttttttctc acgagctgtt tattcatcaa
     3181 ccgggggtaa tttacgcggt ttgcgcctcc gaacagtgtt gtcatctttt ttaaaaaaag
     3241 gctgtatttc aatcatccca catccaaaag tagtatttcg ccccaggtgc aaatgttgtg
     3301 cggcatttag tacgttgata gcggtatttg gcaccctgtc atacctgatg cgtccggtcc
     3361 agcctttaag aggataacca cggtctctat caatactcca ttttcgcaat cgctgttcca
     3421 ctatgccggc accttgtgcc aaatccaagg tttcctgaat gaattcttcc tgcggtaaga
     3481 cagcatttcc ataatacaaa gccatggcac gtatgcgttc tattgcatag cgcagcaaca
     3541 tatcgaaagt aattgtgccg ctttcttgca tttgcttgtc gttagtccaa aaaggcgttt
     3601 taaaatcgat ggtaatatgc tgaaaattat tgtcggtata tccccaatct aataataatg
     3661 tagtttcatg agcgagcacg atatcgagct cccatttcaa ttcaccacta cccaaaccgt
     3721 tggcacacag agcctctaca atttctacaa tctgattcat gtcgccaggg ctattgccgg
     3781 ctacatgcag cgtccactca agcacatcgt cgtatgacac ccgccttttt tcccaatcga
     3841 aattgcaacc gataaaatat ggccttggcg ggtctttata tcttttagcc accatcgggt
     3901 caggagattg agcaggatta taccaaggtt gatacaaaca gttgcaagtg ctgcattctg
     3961 tttgattgcc cggacggtaa tcattgcaca aggctcttct aatgctatgt cccaatgctc
     4021 catgtagcgt atgacccata aatcctgaaa taaatccatc ttccagggcg cgcatgcgaa
     4081 tttgataatg ggtgattggt ataatggtgg gtgccggggg tgacattggt tgaaaagcgt
     4141 aatacacgaa gttaggcaat aataggcatt gccaaggcag ccataacggc aaattgctta
     4201 agcaaagaaa tgtctgatgt tatttcagcg cggttttcga taaatacaga cttcaccctt
     4261 tctttatgac aatgacgata tttttggcca ctgccacata agcatggtga gtttcgatgc
     4321 ggcggtcctc cggcaacaat tttttcgagc agacgaataa tttcaaaaat attggttgtg
     4381 gcaaaaagat cggaatagta tgcataaaga ccggcaatac cgtgttgcca ttcatcccga
     4441 aagaacccat tttttctgcg ataggtttga tgaaaaaaat agggaagtac aaagtcatcc
     4501 ataaatgaaa ccaggctaaa acccatgtta cataatttgc gttcttcggg aggtatacaa
     4561 aggcaacaat ttcctgtttg ctcatacata tgccaatcaa tgtttttcgg tattttgcca
     4621 ccggtttcaa acaccaaagg aaatttatga ggaaattgcc cgcacagtat tttaatttga
     4681 taattatcga ttggattctc cttctgatca ataagcgtaa ttgtgccgat gagtgcaggt
     4741 tcgccgttaa tatccacaag tgatagcagc ggatgtttta taataacacc cgctgcttca
     4801 gcaataaaac gatttttatc cggcataggg ctttgaggat tttatgccgg caattaaggc
     4861 cgggtttgag agatcggggt ctatcttgtc ctcccccaaa gggaacctgt cgccaagatg
     4921 tttacgccac agcttactgg catttaagaa gttgctttcc cgaatggcgg catctgcgtc
     4981 aactaaaaag gcatctaagg cgtttaagaa attctttttt cttgtatcat catactctgc
     5041 aaacaagttg tcattgggtg tagccggcac aatacaagtg aatgacgcta aaagtgtttt
     5101 ctgtatttct ttcaatgtgt ctcgtaagtt aatgtcatcg cgatcattgt aaacaatttt
     5161 agacttagca ttacccgcca atatggtcat tgcaagtcca ttgggcattt tattgcgttt
     5221 atagtcgcac caagctttta agtatttaac gatcttaata agatgtccgg cttcatcttt
     5281 ttgtgccaca aaccagtcga ccatttcttt tggatcatct gcaccccaac catttgtatt
     5341 tgttgctaaa taatacgata gtttgtcttc cgagtaatac accggcatgt ccaattcgta
     5401 atcgccggcg aagatgagtc gtatacattt ttcacgatgc tccggagggg tttcggtatg
     5461 cccggttact gcttgtaaaa cccacgcctg cagggtattt ggtgttacat ccggctttct
     5521 aaagaagtag atgccgtcgt caacatcgca tatatcatct ttcgttcgta tcccggtttt
     5581 catcttatta gacccttgga tataaaattt tgggctgtaa tctgggtgct tctctgtaaa
     5641 ccaatctatt atacgcgttc tgacggcctc tctagatttg gccattttgg cctgtttagg
     5701 gccgggtatg ctgatttcgt tgttaaattg ttcgaataaa ttatgattat ttgccatttg
     5761 cttgaatttt ttttgattaa ttgatatttg tttttttctt tgtcataaat aaaaaccgcc
     5821 ttcatccaat ggatgtagga atgaattaac actatgaaag gtttttttta gagaggacaa
     5881 ttgcagttgt cctctttttt attttattgg tcgcggatca tttattgata tagtttaaat
     5941 tattttattt tgctttgcca tcatacgcga aatgttcaat gtgttagtgt gatatcgctt
     6001 accctctcgt ttgattagtt caatttgttc taatttattt agatctcgtt gaatagtttt
     6061 aagggaaatt ccaccatata gttttgccag agggatagat atttcaccca cctcctcggc
     6121 gctaaattct ttttttagag gcagattaag ggctagtcta cgctgccttc tgaaaacaac
     6181 atttcgtttg gtgtttttca aattatcgaa tgtgtcgtaa acatgtcgtt gccaagtatt
     6241 ttcaaattga ctgttttgaa tttcctttaa ttttccttgt agcccatctc tatatccaac
     6301 taaagcgtat tcaataaatg aagtaagcga ttcatctttt gccgccttgt caagatagta
     6361 gtaatattga ggccttgttt gattgtaata atttgataag atatgtgatg caatatcagg
     6421 gtttcctcca cgaattaaaa tataaaactc aagaagtcta ccggttctcc cgttgccatc
     6481 gctaaaaggg tgtatccatt caaggtaaac gtgacacacg attgcctgaa taagaacgtt
     6541 gtgaaacgcc tgtcttttct ttccaaacgc aaactcatcc tgcatccaag cgcaaagttt
     6601 gtctattaga ggttctacat cacgatggtc gggacatcga tacctattaa caataacctc
     6661 gttgtttcta aacttacccg gaattgccgc aaatgtctcg ccgagatttt tacctataag
     6721 ttgatggaat gttaagatta attctttaga tatcagttta ttggcctggc catctaatac
     6781 gtcaaagagg atactattta aagcgtcaac cacgttcttc acttcgcgtt gttggtagcc
     6841 ttgtgatgga ggcaggtctt ctcctgctat aacacgctca atttcttcat cagatagcgt
     6901 attgccttca attgctgtcg ttgattgggc cccctttaaa agagaaactt tcataagctc
     6961 atcatagtag tggggtaaaa tgggcgtgtt agtgatggcc tctgcatagg ccttacattc
     7021 accaattatc cgccaagcgt cagccgtaag ctcccagtga cgcctgaagg caatatgagg
     7081 gtattgacga gctagttcag acatatcttc gtatcaatat tttgacaaca gttgggacaa
     7141 atatagtaaa tatttgtcta aaatcaaaat tattaagtat cttgattaaa aaatattggg
     7201 ccataaaata agctaaattg cgtattttat gtagtaattt aagtgtttct agtgtgtttt
     7261 tgacatcgaa aagccagcaa atcggttgca aaagttatag taaggcaagg agactgcttt
     7321 tactgttagt attgacaatt tgcgccaaaa ctgctaaact tgccttaatc tctgacccca
     7381 aaaaaattaa atggccgacc ataaagctaa acacttagaa ttcatccaag aggctattaa
     7441 gcggatgaat acgttgtcat ttcagatcaa aggttggtgt atcacgttgg tttcagccat
     7501 caatgtgctt gcagccaagg atgtagacca cgatcatata ttgcttgtat attggatcgt
     7561 cccaattttt tgggcaatgg atgcattcta cctaagtcag gaaagacaat atcgcacctt
     7621 atataacgaa gttaggctta aaaaggaggc agacattgac tttaatatgc aaacccaagc
     7681 atcaatactg ggaaaaaaca gttggatttc agctatgttt tctgtttcaa actggccggt
     7741 ttaccttata ttattagggg taacagtata tataatgttc aattgttagg aatgatttcg
     7801 gatccgcatg taattgagct gtgcaaaagg ttgcttgatc aatgggaggg tgatgacata
     7861 ccttatgaaa accgaaaagg gtacaatata ggggcattga tagtaagccc cgaaaacaaa
     7921 gtcgttgcat ttgaacgaaa tgcggtaaat gtaaatgcca atgtgaccga gcatgctgag
     7981 ttaaatgtca ttcgcaatta cctgcgcaaa tccgg
//
LOCUS       DAOQHY010000142         8009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_4641_length_8009_cov_5.919034, whole genome shotgun sequence.
ACCESSION   DAOQHY010000142 DAOQHY010000000
VERSION     DAOQHY010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 8009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8009
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4641_length_8009_cov_5.919034"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PLL28_11155"
     CDS             complement(<1..264)
                     /locus_tag="PLL28_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69926.1"
                     /translation="MKLILFIVLAFFVEFAYSQAPEIEWQNTIGGGSSDNLSCISLCH
                     DGGYIIGGSSGSTIFGDKSEDNIGYLDYWVVKLDSVGNILWQNT"
     gene            complement(292..2178)
                     /locus_tag="PLL28_11160"
     CDS             complement(292..2178)
                     /locus_tag="PLL28_11160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69927.1"
                     /translation="MAEKFKILFINISLCASCTFAQSPSIDWQNTIGGSSDDVLNSIQ
                     QTSDGGYILGGSSKSIISGDKTETHAGQDYWVIKLNSAGFIEWQNTIGGSSDDYLNCI
                     QQTSDGGYILAGYSNSLISGDKTENSIGGFDYWVVKLNSTGGIVWQNTIGGTLDDYLH
                     SVQQTSDGGYILGGESKSDITGDKTENNFGLTDYWVVKLNSAGNIVWQNDIGSWSDEY
                     LNTIKQTSDGGYIIGGYSNSGIAGDKSEACLGGNDYWVIKLNSLGVIEWQNTIGGNSE
                     DNLFSAEQTLDGGYILGGWSLSGISADKTDAGIGNYDYWVVKLNSTGGIVWQNGIGGT
                     DADYLYSIQQISDGGYFLGGYSRSVISGDKTEAKIGGNDYWILKLNSTGTIIWQNVIG
                     GSSGDLIQSPNTIDETSDGGFIVGGYSSSGISGDKTEVSLGSTDYWVIKLMGDSVLIC
                     GVPTGLFSDNITSTSAKVHWSIIPTADSYKVYYRISGTGPWTKKTAVSNNKNLPGLTP
                     FTTYEWKVKSNCGPESSDFSEIQTFITLPMKEGNLINNQLKLIRVYPNPTSGNIVISF
                     ENTITRTRAVSITDISGKYYLQTNLLFENGLSKFDISKYPSGIYILKIITDGNEFIQK
                     VVKE"
     gene            complement(3194..3268)
                     /locus_tag="PLL28_11165"
     tRNA            complement(3194..3268)
                     /locus_tag="PLL28_11165"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3233..3235),aa:Glu,seq:ttc)
     gene            3321..4373
                     /gene="gap"
                     /locus_tag="PLL28_11170"
     CDS             3321..4373
                     /gene="gap"
                     /locus_tag="PLL28_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNF69928.1"
                     /translation="MSPKILFRQPTFAPIMSTNYRVAINGFGRIGRIFFRRALLKANF
                     EIVAINDLTDVDTLAHLLKYDSVHGRFQGTVSVENGNLVVNGKTIRVSAMKDPAQCPW
                     KELNIDLVIESTGLFLSREKAELHIQAGAKKVLLSAPPKGDDIKAMVLGVNDEILSPA
                     DKIISNASCTTNCATPMIKVIHEHFHIESAYITTVHAFTSDQRLHDAPHKDLRRARAA
                     TLNMVPTTTGAAKAVTLAFPELKGRVGGAGIRVPVADGSITDITCTVTKPTTIEEVNA
                     KFKEASETYLKGILSYETDPIVSVDIVGDPHSCIFDAELTSVIGNMVKVLGWYDNEYG
                     YSSRLADLTERLATMA"
     gene            4634..5830
                     /locus_tag="PLL28_11175"
     CDS             4634..5830
                     /locus_tag="PLL28_11175"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNF69929.1"
                     /translation="MRTIQDFNFAGKRAVIRVDFNVPLDKATFAVTDDTRLRAALPTI
                     NKILHDGGSVVLMSHLGRPKGGPEDKYSLRHIVAHLSQLLNRDVQFVNDCIGDMVMSA
                     ARQLQPGQVLLLENLRFHPEEEKGDHAFAEQLSKLGDVYVNDAFGTAHRAHASTTIIA
                     NYFNTGNKMFGALMAAEVSAGNKVLHQHEKPFTAIIGGAKVSDKILILEKLLEKADNV
                     IIGGGMAYTFYKAMGGQIGNSLCEEERLDTAKQLLETAKAKGVNILLPVDSITADKFD
                     NDANIGSANSDQIPDGWMGLDIGEKAIADFTAAILKSRTILWNGPMGVFEMTNFSKGT
                     IAIARAIAEATANGAYSLVGGGDSVAAVNKFNLADKVSYVSTGGGAMLEFFEGKTLPG
                     VAAIAD"
     gene            5976..>8009
                     /locus_tag="PLL28_11180"
     CDS             5976..>8009
                     /locus_tag="PLL28_11180"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNF69930.1"
                     /translation="MRFLLPLVCGVLIALQFHECYWWIYGLFFVLFIAYLLIGIVTGW
                     TVKFRSQTLVAFLLQGMFLSAGMALVQYQTPWYRSDYFMRDADSSTVFLCRLQKPPSI
                     GEHSIKLEMLVEGKWQQDKYSSASGKTMIYVRRDSTKMDLPVYGDYALIENCFKSPDP
                     PKNPNTFDFPKYLRGIGIAHIARVDASQLVFTGERHQKLWWTFIFRAREMFHDAVMRE
                     VKNGDAQSVGEALVIGTKTAIDPEVQQAYANTGTMHVLAVSGLHVGILFAVLEFLLNP
                     ISWFKRNKYGRVSKFLFIITIIWWYACLTGLSASVNRSAVMFTCLATGKLFSRQNNSF
                     NVLFVSMFLLLVDDARCIAQVGFQLSYLAVGGILFFQPYLQKLWRPKWKILNHIWTMT
                     TVSVAAQISTSPISLFYFHQFPNYFLLSNMLAIPISFVVLVWGLIFFVVNGIPFLGPA
                     TGMALDYSLRLLNGSVMAVNQMPGAVINNIYINEWQLGILYVFVLLLGIALLSKQTMY
                     LFRAAFCLCIVWMLAVFRDVKAREDRRLVAYSIRDHDCMLLQGAGDAILLSDTLIDQN
                     SSIWTLNIRNDALKSGVMPDFALFSLQEPFNGASNVLYGYPWLIGGGKVVFLLDPTSV
                     HQLPKSAPEADWVYILGNPFINLPDLRLRFPNATFVIGGSTSYKRHSYYLNTCN"
BASE COUNT         2364 a         1577 c         1657 g         2411 t
ORIGIN      
        1 cgtattctgc cacaaaatat ttccaacact gtcaagttta actacccaat aatctaaata
       61 tccgatatta tcctctgatt tatccccgaa tattgttgat ccagatgaac caccaattat
      121 atatccacca tcatggcata aggaaataca agataaatta tcagacgaac ctcctccaat
      181 cgtattctgc cactcgattt ccggagcttg agaatatgcg aactctacaa agaatgcaag
      241 aacaataaat aatattaatt tcattattca attaattaaa ataaccaagt tttattcttt
      301 aactactttt tgaataaact catttccatc tgtaattatt tttaagatat aaataccaga
      361 aggatattta ctgatatcaa attttgatag tccattttca aaaagaaggt ttgtttgtaa
      421 ataatatttc ccagaaatat ctgtaataga gacagctcga gttctcgtaa tagtattttc
      481 aaaactgatt actatatttc cgcttgtcgg atttggatat accctaatca attttaattg
      541 attatttatt agattacctt ccttcatggg taaagttata aatgtttgaa tttcggaaaa
      601 atcgcttgac tcaggtccgc aattactttt caccttccat tcgtatgtag tgaaaggagt
      661 taacccgggc aaatttttat tattggaaac cgctgttttt tttgtccagg gccctgtacc
      721 tgaaatacgg taataaactt tataagaatc tgccgtcgga attattgacc aatgcacttt
      781 cgcagaagta gaggtaatat tatctgagaa taaacccgta ggaacgccgc aaattaatac
      841 gctatcaccc attaatttaa ttacccaata atctgtgctt ccaagcgaaa cttctgtttt
      901 atctcctgaa attcccgaag atgaatagcc gccaactata aaacccccat ccgaagtttc
      961 atcaattgta ttaggtgatt gaattaaatc accactagag ccacctatca cattttgcca
     1021 aattattgtt ccggttgaat ttaatttcag aatccaatag tcattcccac caattttcgc
     1081 ttcagtctta tctccagaaa taactgaccg ggaataaccg cctaaaaaat aaccgccatc
     1141 agatatttgt tgaattgaat ataaatagtc agcatctgtc ccaccaattc cgttttgcca
     1201 aacaatgcca ccggttgaat ttagtttaac cacccaataa tcataattgc caataccggc
     1261 atcggtttta tctgctgaaa ttcctgataa tgaccagccc cctaaaatat aaccgccatc
     1321 aagtgtttgt tcggcggaaa ataaattgtc ctctgagttt cctccaattg tattctgcca
     1381 ttcaattacc cctaatgaat ttagtttgat cacccaataa tcatttcctc ctagacaagc
     1441 ttctgattta tctccagcga tcccggaatt cgagtaacct ccaatgatgt agcctccatc
     1501 agatgtttgt ttaatagtat tcagatattc atcagaccaa gagcctatgt cattttgcca
     1561 cacgatgtta ccggctgaat taagtttaac tacccaataa tcagtgagtc caaaattatt
     1621 ttcagttttg tctcccgtaa tgtcagattt tgattcgccc cctaaaatat aacctccgtc
     1681 agaagtttgt tgaaccgaat gtaaataatc gtctaaagtc ccacctattg tattttgcca
     1741 aacaatgcca ccggttgaat ttagtttaac cacccaataa tcaaaaccac cgatcgagtt
     1801 ttcagttttg tcacctgaaa ttagggaatt ggaataaccc gccaagatat aacctccatc
     1861 agaagtttgt tgaatgcaat tcagataatc atctgaactt cctccaattg tgttttgcca
     1921 ctcaataaaa ccggcagagt taagttttat cacccaataa tcttgaccag catgagtttc
     1981 cgttttatca ccggaaatta ttgatttgga agacccaccg agaatatagc cgccatcact
     2041 tgtttgctgt atagaattta gtacatcatc tgagctacct ccaatagtat tttgccagtc
     2101 aatacttggt gattgtgcaa atgtgcaact tgcacataat gagatattta tgaacaatat
     2161 tttaaacttt tcagccattc agttggtttt accaaactaa agttattttt tttaattggt
     2221 aaaagcaact cacaagatgg atttattagt aaaattttcg aaaaaatcat gatgattcca
     2281 aatccttcaa cactttgagg cagtttataa cagcactaaa aaatatcata tgaataattg
     2341 gtaagaaatc cgatttactg cgggaaaatt ttaatcaaag catacaggaa cgagccggaa
     2401 gaaatagtag ggggaataca tgaacctatt gtgagcgaac aggtgttcga tactgttcaa
     2461 aatgtattgg cgaataagaa gaaaattaag gcgaagccta aaactaaatc agattttcta
     2521 ccgcttcgcg gatttttggt atgtcctgac tgcggaaaaa atctaacagg cagcgcatca
     2581 aaaggaaatg gcggcaggta tttttattac cactgccagc ccgggtgttc tcaaagggtg
     2641 aaagctgatg tcgttcataa ttcattttcg gaatggttgg attcattttc ctttaaaccg
     2701 gaagtagctg agctctacct cgctatcatt gaggacactt tcaaacatga tgaagagggt
     2761 cgtattaaag agatcaagag attaaagtgt acaatagatc aaattagctc caacctgcta
     2821 atggtcgaaa agaaatatgt caatgatgag attgaacgag attcgtatct ccggcttaaa
     2881 gaatcatata agcaggaaga atatgaatta agagaacatc ttgcaagatt gcagtctacg
     2941 gattcagcat tcatggaata tatgaaatat gggtgttcag ttttatcaaa tctacgtcaa
     3001 tgctacgata cagctgatgt agatgcaaaa caaaagctgg ttggttcgat attccctgaa
     3061 aaactcatta ttcagaatgg aaagtatcga accacaaaag agagtgaact gatgcggctg
     3121 ctttgcttgt ccggtgcgga tttcggaata tctgaaaata aaaattcccg caaaagtgcg
     3181 ggaaaattcg ctatggtagc ccgtagggga atcgaacccc tgttccgaga atgaaaatct
     3241 cgtgtcctaa cccctagacg aacgggcctt gtccgtaaaa gagagtgcaa aagtataaga
     3301 tttttgcaaa acagggaaat atgtcaccaa aaattttgtt ccgacagcct acctttgctc
     3361 ccattatgag tactaactat cgcgtggcca tcaacggttt cggtcgtatt ggtcgcatct
     3421 tcttccgcag agcattgctc aaagcaaatt tcgaaatcgt ggctatcaac gaccttaccg
     3481 acgtggatac cctcgcccat ctgttgaaat atgattcagt gcatggcaga tttcagggta
     3541 cggtgtccgt ggaaaacggt aacctggtag tgaatggcaa gaccattcgt gtttccgcca
     3601 tgaaagaccc ggcgcaatgc ccgtggaagg aactcaatat cgacctggtg atagaatcta
     3661 caggcctctt cctctcacgc gagaaggctg agctccatat tcaggccggt gctaaaaagg
     3721 tattgctgtc ggcccctccg aaaggcgatg atataaaagc catggtgctt ggagtaaacg
     3781 atgaaatctt atcgcctgcc gataaaatca tctctaacgc ttcctgcact accaactgcg
     3841 ctacgcctat gatcaaggtg attcatgagc attttcatat agaatctgct tatatcacca
     3901 ccgttcacgc tttcaccagc gatcagcgcc tgcacgatgc gccgcataag gacctccgca
     3961 gggctagggc tgctacattg aatatggtgc ctacaacaac aggtgctgct aaagccgtta
     4021 ccctggcctt ccccgaactg aaaggtcgcg ttggcggcgc cggcattcgc gttccggtgg
     4081 ctgatggctc tattacggat atcacttgca ccgtaaccaa accaacaacc atcgaagagg
     4141 tgaatgcgaa atttaaagaa gccagtgaaa catacctgaa aggtatcctc tcctatgaaa
     4201 cagatcctat cgtttctgtc gacatcgttg gcgatccaca ttcatgtatt ttcgacgcag
     4261 agcttacttc tgttatcggc aatatggtga aagtgctggg atggtatgat aatgaatatg
     4321 gttattcaag cagattggca gatttgaccg agagattggc aacaatggcg tgaattggta
     4381 gtgaatggtg aatggtgagc ctgcctcgcc gataggcggg tggtgaatgg tgagtggtga
     4441 atggtgagcc tgcctcgccg ataggcgggt ggtgaatggt gaatagtgaa tagtgagtgg
     4501 tgagtagtga ataatacttg atacctgata cctgatactt gataccaatt caagatacca
     4561 attctcagtg aatgttgagc aattaactga taataaaatt acttggagat ttaattaaaa
     4621 aatcattata aaaatgagaa cgattcagga ttttaatttt gctggaaaga gagcggtgat
     4681 acgtgtcgat tttaatgtgc cgctcgataa agcaacattc gctgttaccg acgatacacg
     4741 ccttcgcgca gcattgccta ccatcaataa aatattacat gatggtggtt ctgttgtgtt
     4801 gatgtcgcat cttggccgac caaaaggcgg acctgaagat aaatattcac tgcgtcatat
     4861 cgttgcgcat ttgtcgcaat tgctgaatcg cgatgtgcaa tttgtaaatg attgtattgg
     4921 cgatatggta atgtcggcag ccaggcaatt acaaccggga caagtattat tattagagaa
     4981 tttgcgtttc catccggaag aggaaaaagg tgatcatgca tttgcagaac aactgtcgaa
     5041 attaggagat gtatatgtaa acgacgcttt cggaactgct caccgtgcgc atgcatctac
     5101 aacaattatt gcgaattatt ttaataccgg aaataaaatg ttcggcgcct taatggcagc
     5161 tgaagtaagc gcaggaaata aagtattgca tcaacacgaa aaacctttta ccgctatcat
     5221 tggtggtgca aaggtttctg ataaaattct catcctcgag aaattattgg agaaagcaga
     5281 taatgtaatt atcggtggtg gaatggcata tactttctac aaagcaatgg gcggacaaat
     5341 cggaaattca ttgtgtgagg aagaaagatt agatacggct aaacaattat tagaaacggc
     5401 aaaggcaaaa ggcgtaaata tattattgcc tgttgacagc attacagcgg ataaatttga
     5461 taatgatgcc aatatcggat ctgccaacag cgatcagatt cctgatggtt ggatgggatt
     5521 agatattggt gaaaaagcga ttgccgattt tacagctgca atcctcaagt cgcgcactat
     5581 tttatggaac ggaccaatgg gtgttttcga aatgaccaat ttctcaaagg gaacaatagc
     5641 tattgctcgt gcaattgcag aagctacagc taatggtgca tattctcttg ttggtggcgg
     5701 tgatagtgtt gcagcagtaa ataaattcaa tctcgccgat aaggtcagct atgtatctac
     5761 aggtggtggt gccatgctcg aatttttcga aggaaagacc ttgccgggtg tggctgcgat
     5821 agcagattaa aaaaaacttt ctaatcgctt acgcccttat cgttagcgga aaattaaaat
     5881 gacctccttc gggaggtttt tttatttgta tctgctgttt acatttatcg ccttgtttac
     5941 cccttatgcc tgaacattgg caccatattc cttttttgcg ttttctactc ccacttgttt
     6001 gtggcgtatt gatcgcattg caatttcatg agtgttattg gtggatttac ggactctttt
     6061 tcgttttatt tattgcatat ctattaatcg gcatagttac aggttggact gtaaaattcc
     6121 gaagccagac acttgttgct tttttattgc aaggtatgtt tctaagcgcc ggtatggcat
     6181 tagtgcaata ccaaacacca tggtatcgca gcgattattt tatgcgtgat gccgatagca
     6241 gtacggtatt tctatgtcga ttgcaaaagc cgccaagcat tggagagcat agtattaaat
     6301 tggagatgtt ggttgaagga aaatggcaac aggataaata ttcctctgca tcaggaaaaa
     6361 caatgatata tgttcggcgt gatagtacga aaatggattt acctgtttat ggcgattacg
     6421 cattaattga aaattgtttc aaatctccgg acccgccgaa aaatcccaat acctttgatt
     6481 ttccaaaata tttacgtggt attggcatcg cacatattgc ccgcgtagat gcatcgcaat
     6541 tggtgtttac aggtgagcga catcaaaaat tgtggtggac atttattttt cgtgcgagag
     6601 aaatgtttca tgatgctgtg atgcgcgaag tgaaaaatgg cgatgctcaa tctgttggcg
     6661 aagcattagt gataggcaca aaaactgcca ttgatccgga ggtgcaacaa gcttatgcca
     6721 atacaggcac catgcatgtg ctggcagttt ccggattgca tgttggtatt ttattcgcgg
     6781 tattggaatt cttattaaat ccaatatcat ggttcaaaag aaataagtat ggaagggtgt
     6841 caaaattctt atttattatt acaataatat ggtggtatgc gtgcttgacc ggtttatctg
     6901 ccagcgtaaa caggtctgcc gtaatgttca catgcctggc taccggaaaa ttattcagca
     6961 ggcaaaataa ttcgtttaat gtattatttg tgtccatgtt tttattattg gtagatgatg
     7021 cccgatgtat cgcacaggta ggatttcaac tatcatatct cgcagtgggc gggatacttt
     7081 tttttcaacc ttatctgcaa aaattatggc gaccgaaatg gaagatttta aatcatatct
     7141 ggacaatgac gactgtttca gtagccgcac aaattagtac atcgccgata tctctgtttt
     7201 atttccatca gttcccgaat tatttcttgt tgtcgaatat gctggcaatc ccaatatcat
     7261 ttgttgtgct ggtttgggga ttgatatttt tcgtggtgaa tggaattccg tttttgggac
     7321 cggcaacagg tatggcttta gattattccc tgcgattatt gaatggttct gttatggcag
     7381 ttaatcagat gccgggagcg gtgattaata acatttatat caatgaatgg cagttaggca
     7441 tcttgtatgt ttttgtgctg ctattgggaa ttgcactttt gagcaagcaa actatgtatc
     7501 tgtttcgtgc tgctttttgt ttatgtatcg tttggatgct ggctgttttt agggatgtta
     7561 aggcccgcga agacaggaga ttggttgcct acagtatcag agaccacgac tgtatgttgc
     7621 tgcagggggc gggtgatgcc attttattat ctgataccct gatcgaccaa aacagctcca
     7681 tttggacact gaatatcagg aatgatgcat tgaagtctgg tgttatgccc gattttgccc
     7741 tttttagcct tcaggagcct tttaacgggg cttctaatgt cctatacggc tatccttggc
     7801 taatcggagg gggtaaggtg gtttttctgc ttgatcccac ttctgtacac caactgccaa
     7861 aaagcgcccc tgaagcagac tgggtgtaca ttctgggcaa tcccttcatc aacctacccg
     7921 acctgcgatt gcgcttcccc aatgccacct tcgtaatcgg cggaagtact agttacaagc
     7981 ggcattcata ctatttgaat acctgtaat
//
LOCUS       DAOQHY010000143         7940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_4711_length_7940_cov_4.867597, whole genome shotgun sequence.
ACCESSION   DAOQHY010000143 DAOQHY010000000
VERSION     DAOQHY010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7940
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4711_length_7940_cov_4.867597"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..858)
                     /locus_tag="PLL28_11185"
     CDS             complement(34..858)
                     /locus_tag="PLL28_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69931.1"
                     /translation="MDSRKVPEVYFHVGLGKTASTWLQNKVFGKFQGVLYIHPTAYTQ
                     WRKAVVNTIYDKVLISREMDRQLEREVSAFAAVRPDTHAIIVMRTNAQWIASQYRRFL
                     KNGYCFTFEEFFDVEHDNGFWKRDDVFLFPKIEILQKYFAHPPLILTYDQLKQDPKEF
                     VLAMSNYVGVQVDINAISFDKHHASYSEKQLKWMLQVGKYLFSKKIQDPSLPRWRKWI
                     RRRPRMWMCYIVLYSAYLIPNALISKQPLITKEALDKIDRFYANDWESVLRKMSMN"
     gene            998..3283
                     /locus_tag="PLL28_11190"
     CDS             998..3283
                     /locus_tag="PLL28_11190"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF69932.1"
                     /translation="MKRLFTSLLTLMSVYALSGQSGTIRGFVYDIENGEPVPFVNVIV
                     MQSDNIGAASDENGFFSIPEVPVGAQRLILTYIGYDTLIHPITVEAGQIYNVKLNMTQ
                     SGISLSTVTVSSKVTKKLEEVQISTTTITPLQIKRLPSVGGEPDLAQYLQVLPGVIFT
                     GDQGGQLYIRGGSPIQNKVLLDGMVIYNPFHSIGLFSVFETDIIRNVDVYTGGFSAEY
                     GDRVSAVVDITTRDGNKKQTVGEVSASPFLARVLLEGPLKKPSAESSSVTYILTAKHS
                     YLDKTSEIFYPYVNSQEGYSLTQGIPYTFTDLYGKISINSESGNKFNVFGFNYRDSVN
                     YSSITNYRWTASGAGTNFVIVPSASNILIEGVFAYSNYNMTQNEADEKPRTSNVGGFN
                     GGVDFTYYLGSGDLQYGVEVIGFNTNYEFFNRLGIRTGQEQFTTELGSYVKFRKSFNK
                     KFVIEPSIRLQYYASLSEFSPEPRIGMKYNITDRVRIKFAGGLYSQNLISSKSDRDVV
                     NLFTGFLSGPDDDLYTIDGEEAKSKLQRSTHAIAGIEYDPTDFIELNIEPYIKYFNQL
                     IEINRNKVTPRDADFATETGQAYGIDFSMKYDRKKLYIWLAYSLGFIERNDGEQVYPP
                     HYDRRHNMNFVTTYTLGKRWELSARWNLGSGFPFTQTAGFYESVDFLDGISTDYTGTN
                     GNLGIIYSAQLNGGRLPYYHRLDLAVKHNIVFANKNKVEINAGVTNVYNRDNIFYFDR
                     VRYERVNQLPVLPSLSMSYSF"
     gene            complement(3332..3413)
                     /locus_tag="PLL28_11195"
     tRNA            complement(3332..3413)
                     /locus_tag="PLL28_11195"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3377..3379),aa:Leu,seq:cag)
     gene            complement(3501..7055)
                     /locus_tag="PLL28_11200"
     CDS             complement(3501..7055)
                     /locus_tag="PLL28_11200"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNF69933.1"
                     /translation="MPEDRQKRLLGTRFQAWELLSTPLVFVDKSFNILHFNQSFRNIL
                     QCRDAVFPARELQEVFPGFLHAWFELGGMGDQTGILLSSQWEPSCSKYYDLHVSQISQ
                     ESEHIEGYAVICTEVTERVLAHHKDKDLSGQYQTFLNLTQSSVVIHAAGIIRYCNDIT
                     ASMFEFESVDEMIGLSVWPFVEEGSRDVTMQRIREMIINNKPAGVIEVQFVKKNGVAF
                     EVEVFSFPVLHEGEVAVKTFITDISHRKRTERQIIAVKEQYLNLVENITDIIFQTDND
                     ANFTFLNRVWESYTGFQIDDTIGRSCFEFLNHPQNTSLFYTKVRRLLEYGIKEFQYDL
                     LLSVKNGEPRFVQVSLKPLVDQKGLITGINGIMRDIHARKLADIEVRKIQKTLKHHQH
                     TLASLTKEESMINGDFHGALKSISRVAAHTLDVSAVNIWKFSPDFSTLTCVINYDRQS
                     DAYSSLQEFHINQFPAYFKLLINERFIISDDVQSDDRVIEFRDIYLIPQHVISMMDVA
                     ISNGDQLWGIMCFENRHSWHKWTLEDQSFARSLTDFVSLAFKSSQLNTAESALREKEY
                     HYTNLVEQAQDAIMIIDADDNFLEVNDAACRISGYAKEELLAMQVGQILPKGRIEFYR
                     KPNNDTVENKYLFGEQTIIRKDGTERTVEISARVFSDGRLQGIVRDVTERKLQDKALR
                     ESEARLDLALKGAEMGIWDFYIRENKMVHNKRWAEMLGYYFEDTVVTEEYWDKFIHPD
                     DRDKAYANFDEHIKGKRPIYESEIRMLASDGQWRWILDKGRVVEWDAEGKPLRASGIH
                     QDITAIKTYQQQILNQAGFMQQLVNAIPNLIYVKNAKNEFITVNNALAGFLGTTAEDL
                     LQYPHGQQRNYSAVLNQLTACDSEVFLKKDIIITHEEEIPDVQTGRLIWLKSIKVPLI
                     DHQGVLSEVLSVSMDVTALKQKENEISLLNDGLEIKVLERTNELEIANKELETFNYSV
                     SHDLRTPLRSIDIFAYLLDKYNKETLNPEALENIRQIRSSVVKMSTLIDNLLIFSKMG
                     RSDKRMDIVPVQKLVEEVLSELNKHADLSHYTILVRDLPVLTGDYDMLRQALMNLVSN
                     AIKYTQTRKRPIIEFFSTSDEQAHTIAVRDNGVGFSAELKDKLFQAFRRLHSEEQFDG
                     TGVGLAIVERIIKRHNGRVWAESEEGQGSTFYFSIPRG"
     gene            complement(7125..>7940)
                     /gene="bshC"
                     /locus_tag="PLL28_11205"
     CDS             complement(7125..>7940)
                     /gene="bshC"
                     /locus_tag="PLL28_11205"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis BshC"
                     /protein_id="HNF69934.1"
                     /translation="FPYEAQAHPRDINLFYLDNHGRNRIIADEKHEMFLVKDTDVTMT
                     REEILYQLREFPERFSPNVILRPVFQQKLLPSVAFIGGGGEIAYWMQLKSVFEYHQVQ
                     YPMLLMRDSFMIIDHATNKKIQKLQLNITDLFEDVSGLTGEYVRRNSGIALQLDAEKA
                     VLAKMIEGLKEKAKLIDSSLDSAVMAEGQAMLNSLHKLESKLLKAEKGKMEVQLTQLT
                     GILNKLFPSGGLQERYDNMIPWYLRFGPSLIEELIANSCQPAAAFHILTEEMA"
BASE COUNT         2246 a         1703 c         1595 g         2396 t
ORIGIN      
        1 gcttggctcg agatacttct aaaattgtct gaattaattc attgacattt tcctcaatac
       61 actttcccaa tcattggcgt aaaacctgtc aattttatcc agggcttctt ttgtgatgag
      121 tggttgtttg gaaataaggg cattgggaat gaggtaggca gaatacaaaa cgatatagca
      181 catccacatg cgcggtcggc ggcgtatcca ttttcgccag cgtgggagtg aagggtcctg
      241 aatttttttg ctgaataaat acttccctac ctgcaacatc catttcaatt gcttctcact
      301 gtaggatgca tgatgtttat caaatgagat agcattgatg tctacctgca caccaacata
      361 atttgacatg gcaagtacaa attctttagg atcttgtttg agttgatcgt aggttagaat
      421 taagggtgga tgagcaaaat atttttgcaa aatctcgatt ttggggaaaa gaaaaacatc
      481 atcccttttc caaaatccat tatcgtgttc cacatcaaaa aattcctcga aagtgaaaca
      541 atagccattt tttagaaatc gtcgatattg cgaagctatc cattgagcgt ttgtacgcat
      601 cacaatgatg gcatgcgtat cgggtcgcac tgctgcaaat gcactcactt ctcgttcaag
      661 ctgccggtcc atttcccgtg agatcaacac cttgtcgtat atggtattca ctaccgcctt
      721 tcgccattgg gtatatgcgg ttgggtggat gtaaagcacg ccttgaaact taccgaatac
      781 cttgttttgc aaccaggtac tggcagtttt tcccaaacct acgtggaaat acacttcagg
      841 gaccttccgg ctatccatcg acggcgaaat tcagcaaaat aaggggtgca cgcaaatcaa
      901 aggctgtgca tagctccggt ttggtgtcaa agccttgtca aattgggcga tttgacgcca
      961 tgattgcacg atttcaagta tcttcgctgt gcaaaatatg aaaagattat ttacttctct
     1021 ccttacgctg atgagtgttt acgccctttc aggacaatcg ggcaccatcc gcggatttgt
     1081 ttacgacatt gaaaatggtg aaccggttcc ctttgtgaat gtgattgtga tgcaatccga
     1141 taatattggg gctgcctccg atgagaacgg cttcttctca atacctgaag tacctgttgg
     1201 tgcgcagcga cttatcctca cctatatcgg atatgataca ctcattcatc caattacagt
     1261 tgaagcaggt cagatttata atgtgaagct caatatgacc caatcgggga tctctttgag
     1321 tacagttact gtttcaagca aggttaccaa gaaactggaa gaagtgcaga tttctactac
     1381 cacgatcaca cctttgcaaa tcaaacgttt accatcagtt ggtggcgaac cggatcttgc
     1441 gcagtatttg caagttttac ccggtgtgat ttttacaggc gaccagggag ggcaattgta
     1501 tatacgcgga ggtagtccga ttcaaaacaa agtgttattg gatggtatgg tgatttataa
     1561 tccttttcac tccattggtt tattcagtgt tttcgaaaca gatatcataa gaaacgttga
     1621 cgtgtatacg ggaggattta gtgctgaata tggcgacagg gtttcagctg ttgtagatat
     1681 tacaacgcgt gacggtaata aaaagcaaac cgttggcgaa gtatctgcca gtccgttttt
     1741 ggcacgtgtg ttattagaag gtccattaaa aaaaccaagt gccgaatcca gcagtgtaac
     1801 ctatatcctt acagcaaaac acagctacct cgataaaacc tcggaaatat tttatcccta
     1861 tgtaaatagt caagaaggtt attcgctcac acaaggtatt ccatatacat tcacagattt
     1921 atatggtaaa atttccatca attcagaatc cggaaataaa ttcaatgttt ttggattcaa
     1981 ttatcgcgat agtgtaaatt attcgtccat cacaaattac cgctggacag ccagtggtgc
     2041 cggaactaat ttcgtgattg ttccttcagc atccaatatt ttgattgaag gtgtttttgc
     2101 atacagcaat tacaatatga cacagaatga agccgatgaa aaaccgcgca ccagtaatgt
     2161 cggcggattt aacggcggtg ttgattttac atattacctc ggttcaggcg atttacaata
     2221 tggtgttgaa gtaattggtt tcaataccaa ctatgaattc ttcaatagac ttggtatccg
     2281 cacaggacag gaacaattta ctactgaatt aggctcttat gtgaaattca gaaaatcatt
     2341 taataagaag tttgtgattg aaccgagtat tcgtttacaa tactatgcat cacttagtga
     2401 attttcaccg gagccgcgca ttggtatgaa atacaatatt acggatcgcg ttcgtataaa
     2461 gtttgcgggc ggactttaca gtcagaacct catcagcagc aaaagcgatc gcgatgtggt
     2521 gaatttattt acaggtttct tatcaggtcc tgatgatgat ctgtatacca tcgatggcga
     2581 agaagctaaa tcgaaattac agcgttcaac acatgctatt gccggtattg aatatgatcc
     2641 taccgatttt attgagttga atattgaacc ttatatcaaa tatttcaatc agctgataga
     2701 aatcaaccga aacaaggtta caccacgcga tgctgatttt gctacggaaa ccggtcaggc
     2761 atacggcatt gacttcagca tgaaatatga tcgcaaaaaa ttatatatct ggctggcgta
     2821 ttcactcgga tttatcgaac gcaatgatgg cgagcaagta tatccgccgc attacgatcg
     2881 ccgtcataac atgaactttg ttaccaccta tacattaggt aaacgctggg aattaagtgc
     2941 acgttggaat ttaggatctg gattcccatt cacacaaact gcaggatttt atgaatctgt
     3001 tgatttcctg gatggcatca gcactgatta caccggcacc aatggaaatc ttggaattat
     3061 ttattcagcg caattgaatg gcggtcgctt gccgtattac caccgtctgg acctggcagt
     3121 aaaacataat attgtatttg cgaataaaaa taaagtggag atcaatgcag gggtaacgaa
     3181 tgtgtacaat cgcgataata ttttctactt tgaccgcgtg cgttatgaac gtgtaaatca
     3241 gctaccggtt ttaccgtcgc tatctatgag ttattcgttc taatgataaa ctatataaaa
     3301 aaagacagcc aatatgctgt ctttttttat gtaccggagg cgggactcga acccgcacac
     3361 ctttcggcac acgcccctga aacgtgcgtg tctaccaatt ccaccactcc ggtttgtgga
     3421 gtgcaaagct aggctatttt attaaaaatc gcaaaatatg ttcagcatgc agatgggatg
     3481 ccggccgttt acaagaggta ttatccacgc ggaatgctaa agtagaatgt gctaccctgc
     3541 ccttcttcgc tttcggccca aacgcgaccg ttgtgacgtt taatgatgcg ctcaacgatt
     3601 gccagaccaa cgccggtgcc atcaaattgc tcctcactat gcaggcggcg gaatgcctgg
     3661 aaaagcttat cttttagttc cgcactgaaa ccaacaccat tatctcttac agcaatagta
     3721 tgtgcctgtt catcggaagt agagaaaaat tcaatgatag gtcgcttccg tgtttgggta
     3781 tattttatgg cattgcttac caaattcatc aatgcctgac gcagcatatc ataatcacct
     3841 gtcagaaccg gaaggtcgcg tactaaaatg gtataatgtg agaggtcggc atgcttattt
     3901 aattccgaca acacctcttc aaccaatttc tgcacaggaa cgatgtccat tcgtttatcg
     3961 ctgcgaccca ttttggagaa aatgagcagg ttgtcaatca aggtactcat ttttacaacg
     4021 ctgcttctaa tctggcggat attttcaagc gcttcggggt tgagcgtttc tttgttatat
     4081 ttatctagca gataagcaaa aatatctatc gaacgcaatg gtgttcttaa atcatgcgat
     4141 acagaatagt tgaatgtttc cagctcttta ttggcaatct ctaattcatt cgttctctcc
     4201 agcactttaa tttccagtcc atcattaagc aaactgatct cattttcttt ttgcttaagt
     4261 gcggtaacat ccattgatac agaaagcact tcagataaaa caccttggtg gtcgatcaat
     4321 ggcaccttaa tagatttcag ccaaatcaat ctacctgttt gcacatcagg tatttcttcc
     4381 tcatgcgtaa tgatgatatc ttttttaagg aacacctcac tatcgcaggc tgttaattga
     4441 tttagtactg cgctgtaatt gcgttgttga ccatgaggat attgtaacaa gtcttcagcg
     4501 gtagttccca gaaatcctgc aagcgcatta tttacggtaa tgaattcgtt ttttgcattt
     4561 tttacataaa tcaggtttgg aatggcattg acaagttgct gcatgaatcc ggcctgattc
     4621 aatatttgtt gctgataggt tttgatggca gtaatatcct gatgaattcc cgatgcacga
     4681 agaggtttac cttctgcatc ccactccact acccttccct tatccaatat ccatcgccat
     4741 tggccatcgc tagccaacat gcgaatttct gattcataaa tcggccgctt tcctttaata
     4801 tgttcatcga aattggcgta ggctttatcg cgatcatcag gatgaataaa tttatcccaa
     4861 tattcttctg tcactaccgt atcttcaaaa tagtatccca acatttcagc ccaacgcttg
     4921 ttgtggacca ttttgttttc acgaatgtaa aaatcccaaa tacccatttc ggcacctttc
     4981 agtgcaaggt cgaggcgagc ttcactttca cgaagtgctt tatcctgcaa ttttctttct
     5041 gttacatcgc gtacgatgcc ttgcaatctg ccatcggaga atacgcgtgc gctgatttcc
     5101 acggtacgct ccgttccatc tttgcgtata atagtttgtt cgccaaacaa atatttattt
     5161 tcaacagtgt cgttgtttgg cttgcgataa aattcaattc gcccctttgg caatatctgc
     5221 cccacctgca tggcaagtaa ttcctctttt gcatagccgg aaattcggca tgccgcgtcg
     5281 ttcacttcca gaaaattatc atcagcatca atgatcataa tagcatcctg ggcttgttct
     5341 acaagattgg tataatgata ttccttttca cgcagagcag attcagcagt gttcaactgg
     5401 ctgcttttaa atgccaacga aacaaaatcc gtaagactgc gtgcaaaaga ctgatcttct
     5461 aaagtccatt tatgccagct gtgtctgttc tcaaaacaca tgatacccca caattggtct
     5521 ccattgctaa tagctacatc catcatggaa attacatgtt gtggtatcaa atagatatct
     5581 ctgaattcga ttacgcgatc gtcgctctgc acatcatctg aaatgataaa tcgttcattt
     5641 attaacaact taaaatatgc aggaaactga ttgatatgaa attcttgtaa agaactgtat
     5701 gcatcagatt ggcgatcata attaattacg caggtaagtg ttgaaaaatc gggagagaac
     5761 ttccaaatgt tcaccgcact cacatcaagc gtatgagcag caacgcggga aatggatttt
     5821 aaagcaccgt ggaaatcgcc attgatcatg gattcttcct tggtgagtga tgctaatgta
     5881 tgttgatgat gctttaaagt cttctgaatt tttcgtacct caatatcagc aagttttctg
     5941 gcatgaatat cgcgcatgat accattgatg cctgtgatca gtcctttctg atctaccagt
     6001 ggtttcaaac ttacctgcac aaatcgcggt tctccgtttt ttaccgacag caataaatca
     6061 tattgaaatt ctttgatacc atattccaat aagcgacgaa ctttggtata aaaaagggat
     6121 gtattttgcg gatgattcaa gaattcgaaa caggatctac cgatggtatc atcaatctga
     6181 aatcctgtgt aggactccca aacacgattt aaaaatgtaa aattggcatc attgtctgtt
     6241 tgaaaaatga tgtcggttat attctcaaca agatttaaat attgttcttt tactgcgata
     6301 atctggcgtt ctgttcgttt gcggtgactg atatccgtaa taaacgtttt cacggcaacc
     6361 tcaccttcat gcagtacagg gaatgaaaaa acttccactt caaatgccac gccatttttt
     6421 ttcacaaact gcacttcaat cacacctgca ggtttgttgt tgatgatcat ttcccgaatc
     6481 ctctgcatgg taacatcgcg cgacccttct tctacaaatg gccatacact caagccaatc
     6541 atttcgtcaa cactttcaaa ctcaaacatc gaggctgtta tatcattaca atagcggatg
     6601 atgcctgcag catgaatgac taccgacgat tgcgttagat tcaggaaagt ctgatattga
     6661 ccactcaggt ccttgtcttt atgatgggca agcacgcgtt ctgttacctc tgtacatatc
     6721 actgcatagc cttcaatatg ctccgattcc tggctgattt gtgacacatg taaatcgtaa
     6781 tatttgctgc aggagggctc ccattgactg ctcaacagta ttccggtctg gtcacccata
     6841 ccccctagct caaaccaggc atgcaaaaag ccggggaaga cctcctgcaa ttcacgtgca
     6901 ggaaacactg catccctaca ttgcaatatg ttgcggaatg actggttgaa atgaaggata
     6961 ttaaaggatt tatcgacaaa aacaagcggg gtgctcaata actcccaggc ttgaaatcgg
     7021 gttccaagta agcgtttctg tcgatcttcc ggcatcagac aataaaattg atgctacaaa
     7081 tgcaaaaaca tgtattgaat tgcaagcttt tgtgacatga caaatcaggc catctcttcg
     7141 gtaaggatgt ggaaggcggc tgcaggctga caagaattcg ctattaattc ctcaattagt
     7201 gaagggccga aacgcaagta ccagggtatc atgttatcgt agcgctcctg gaggcctcct
     7261 gaggggaaaa gcttgttcag gataccggtg agctgagtga gctgcacctc cattttgccc
     7321 ttttcagcct ttaggagctt tgattccagt ttgtgcaggc tgtttaacat ggcttgacct
     7381 tctgccatca cagcagaatc cagggagcta tctatcagtt tagccttttc tttcaatccc
     7441 tctatcattt tcgccaacac cgctttctcg gcatccaatt gcaatgcaat gccactattg
     7501 cgacgaacat attcgccggt taaaccggat acatcttcaa ataaatcggt aatgttcaat
     7561 tgcagttttt gaatcttttt attggtagca tgatctataa tcataaaaga atcgcgcatc
     7621 aacagcatag gatattgtac ttgatgatat tcgaataccg atttcaattg catccaatag
     7681 gcaatttcgc caccgccacc aataaatgct actgaaggca ataatttttg ttggaagaca
     7741 ggtcttaata ttacgttcgg actaaatcgc tcagggaatt cgcgaagttg atataaaatt
     7801 tcttcgcgtg tcatggtgac atccgtatcc ttcaccagaa acatctcatg tttttcatcc
     7861 gctatgatgc gattgcgacc atgattatca aggtagaata aattaatatc tcgtggatgt
     7921 gcctgtgctt cgtacgggaa
//
LOCUS       DAOQHY010000144         7804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_4852_length_7804_cov_5.436702, whole genome shotgun sequence.
ACCESSION   DAOQHY010000144 DAOQHY010000000
VERSION     DAOQHY010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7804
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4852_length_7804_cov_5.436702"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..1932
                     /locus_tag="PLL28_11210"
     CDS             100..1932
                     /locus_tag="PLL28_11210"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNF69935.1"
                     /translation="MKARSFSSDPDILPGKLEFMTYFVNVHHTLPIVSIAGDTLLELA
                     NGDADLEPIGSIEVFGVDGDSKTRSYGELNSHGQDSWANDQRSIDWVSRDEFGYSRAL
                     KEKFFAQSDREDFQRMIFRAAGDDNYPAAHHTANLGSTHLRDDYIQMLAKEDNLDLDV
                     RTSARCIVYLNGQYWGVYSFREKTDDHDFTDFYYNQGKYDIQYQMTWGNTWSEYGGSA
                     TINAWQDLRDLAELQDLSLDTNYHEITELLDITSLVDYFCVNTSAVCKDWLVYNTAVW
                     RGFNPEGTHHKWGYTLWDLDATFGFYINYSGVPDIGPTAEPCDIVDYDPDFFPPAQFD
                     GKGHVRLLEDLRENAEFEQFYLSRYTDLYNTTFNCENMLFKLDSMIAIIEPEMEMHAN
                     RWYGTYDEWYSNYQDLRTWISMRCEDLYTGMSTCYSLTGPYEVTFNVEPAEAGYLKVN
                     TLEHHVLPWQGTYFGGMENKIIAYANDPDIWAFDHWEAGTTTFSTTWDSTMATLTSDD
                     NITAVFRLKTDINDNEQMAFAVYPTIFEDALMLNYTSTDGSDVTFTMYDTHGRFVASK
                     IFAAPTPGGYAESWDVSSLQLPAGLYLIECVSGDQRGSVKVIKQ"
     gene            2104..2598
                     /locus_tag="PLL28_11215"
     CDS             2104..2598
                     /locus_tag="PLL28_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNF69936.1"
                     /translation="MQLDDLDYQILEQLIHDGRKSFTDIADELSVSVGTVRNRYNTFI
                     ENGLLNIIGRVHPEKIGFNTYAQILIKVRPVEKISAVADNISQLPEVSFLAMTTGAYD
                     LEVNVMCRNNEHLVELMTNHITKMDGIFESSTNIYFKVYKYAQPDLYLLRREGSAIDI
                     NNEK"
     gene            2648..5755
                     /locus_tag="PLL28_11220"
     CDS             2648..5755
                     /locus_tag="PLL28_11220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF69937.1"
                     /translation="MHKPTTQFKFVLVIACWCIAFQLHAQTIITGVVRDSSTNETLIG
                     ANVRLVSDPSVGTSTDLDGAFTLSLQASSGALKISFVGYNEQTISFNGSGTLNVLLSS
                     EHILEEIVVVGYGTQKKSDVTGSISSTRNEDFRDQPISNVAGSIQGKVSGIDIRNASG
                     TPGAGLLVSVRGQYNPLYVVDGIPMISESNSSISTSFDLQGNSVGAGQNISSISDINP
                     DDIESIEILKDASAASIYGARAANGVILITTKRGAAGKTRFSLNYYSGIQQVSRPLDF
                     LSSEEFVDLINEARQNDLNAYEADPSLFGDDYDPALSTNPLPDSWATGVNTNWMDEIF
                     RVAPINNVEFSASGGNDKTKFYISNSYFDQQGIVIESYYKRFNSRINLDHQVSDKLKI
                     GENISLTTSGNRRSLNDNVYTGTVTNAIGASPLMPVYEDDGSYASFEDYQASWLSDNP
                     VLSTKEIIPFTRTNRALASIFADYAFTPKLHFRSTWSADVTFLTDDIYFSPITTDAEA
                     VGGKAMNSAFNNKVWLGENILSYNSELGNNNFLDIIGGFTLQVSNSDYVSISGQGFPA
                     GSGLQDVSSAAIVTGGESFSTGWSLVSFLGRANYTIKSRYLFSLSARVDGSSRFSPNN
                     QFGFFPAGSVGWHMSDEKFWPSGLFITDMKLRMSYGITGDQEIGDFQYISFWSPVGYN
                     GQAGLGPRNLADENLRWQSNKMFNTGLDYELYRGRISGSLEYYIGNKTDLLSDDVISG
                     VTGFGSVTRNYGNIQTSGFELSSNIYVYDRKDFDWTVGVNVSWLHNEIIDLSTDSILL
                     SAYSDLTATHILAEGQAYGSFWGVPYEGVDPETGDPIYTDTNGDGVIDDADAQILGKA
                     TPDWFGGLNTSLRYKKWDAQIAATFTLGNDVYNMIRSTYQTGGWSDYGWDEEYYLYQV
                     YANNTHVIDDRWQQPGDVTDIPRASLYFYNFYQNSGQAIESGSHARIREISIGYTIKP
                     SSIKSFESLRIYAQVQNAYVFTKYTGFDPEVSSTGGDHPETAGVDYAAYPQPRTYMVG
                     FNFKF"
     gene            5772..7166
                     /locus_tag="PLL28_11225"
     CDS             5772..7166
                     /locus_tag="PLL28_11225"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNF69938.1"
                     /translation="MKKIFYFIIAAMALTGFNSCEGFLSQTSTNEVADSDVFTDAAGL
                     ENARIGLYNCLQSQYYYGGYYQLIADAYSDNGTTGGYDNLDLDELGYKEASADNIYIE
                     NVWIAIYNTIYTANQILANIDNIQDETLSDEVSDDIKGEALAIRAMAHFDLLRMFGEH
                     WDLSSSIGIPVVTEVLSTDAQMKRNTVSETYNAILADLITAESMLKNDDYRYDPPIFK
                     GPQFMTQTACQALLARVYRSMGDMSNALDKASEVINTGFASMIDGDQVSDIYNTKFSS
                     EAIFELAFNAQDKSQFNALTYAREDALRSEVSFLAAADLNDFFAGRPDDFRAPLLNFT
                     GNDVSIEPDGRTEKYRGEIFQDNSAFIIRAAEMYLIMIEAKGYPAGVTDMISFTLNRG
                     VTSVDLTSSEAFEQALMNERRAELNFEGDRYFDLAHFGKVSEILGDEVLPCLPIPNRE
                     IGANGGLLEQYPGY"
     gene            7193..>7804
                     /locus_tag="PLL28_11230"
     CDS             7193..>7804
                     /locus_tag="PLL28_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNF69939.1"
                     /translation="MTNSFRSIHPFDQHVIAEYPLMEAKAVDTVLMHSADAFKHWRRT
                     SMQLRADLLLRLAVVLRENKDVYAKIISLEMGKLFKEAQGEVEKCAWNCVYYAENGAA
                     MINPEPAPADGSESYVVFEPIGAVFAIMPWNFPFWQVFRFAAPVLLGGNTIILKHAPN
                     VTGCAIAMQQIFLDAGFPEHVFQTVVIDVDLVENIIAHDIVQGV"
BASE COUNT         2343 a         1500 c         1681 g         2280 t
ORIGIN      
        1 cctgactgtc attgataacg gcgtgggcat ttcggaacag aaccaaagca agatgatcct
       61 gtgtataccg acagtatttt cattgcatca acaaccgttt tgaaagcaag atctttcagt
      121 tctgatcctg atattttgcc ggggaaattg gaattcatga catattttgt aaatgtacat
      181 catacacttc ctattgtttc aattgcaggt gataccctac ttgaattagc aaatggcgat
      241 gcagaccttg aacctatcgg atctattgag gtgtttggtg tagatggtga tagtaaaacg
      301 cgctcgtatg gtgaattaaa ttcacacgga caggattcat gggcaaatga tcagcgcagt
      361 atcgattggg tgagtagaga tgaattcgga tatagtcgcg cattaaaaga gaaatttttc
      421 gcgcaatctg atcgtgaaga ttttcagcga atgatttttc gcgcagcagg tgatgataac
      481 tatcctgctg cacatcacac tgcaaattta ggaagtactc atcttagaga tgactatata
      541 caaatgctgg ccaaagaaga taatttagat cttgatgtgc gcacatcggc aagatgtatc
      601 gtgtatttga atgggcaata ctggggtgta tattctttca gagaaaaaac cgacgatcat
      661 gatttcactg acttttatta caaccaggga aaatatgata ttcaatatca aatgacctgg
      721 ggaaatacct ggagtgaata tggtggttct gcaactatca acgcatggca agacctccgc
      781 gatcttgccg aattgcagga tttatcatta gatacaaatt atcatgaaat aacggaactg
      841 cttgatatca ctagtttggt agattatttc tgcgtaaaca cctccgccgt ttgcaaagat
      901 tggttggtat ataatacggc agtttggcgt ggatttaatc cggaaggaac acaccataaa
      961 tggggataca ccttatggga tctcgacgcc actttcggct tttatatcaa ttattccgga
     1021 gtacccgaca ttggtccaac cgctgaacct tgcgatatcg ttgattacga tcctgatttt
     1081 ttccctccgg cacaattcga tggcaaaggg catgtgcgat tgcttgaaga tttacgtgaa
     1141 aacgcagaat ttgagcaatt ttatctttca cgatatacag atttatacaa tactaccttc
     1201 aactgcgaaa atatgctgtt taagctcgat agcatgatcg ccattattga acctgaaatg
     1261 gaaatgcatg ccaaccgatg gtatggcact tatgatgaat ggtactcgaa ttatcaggat
     1321 ttgcgaacct ggatttctat gcgctgcgaa gatttgtata ccggtatgtc aacatgctat
     1381 tccttgaccg gaccttacga ggtaacattt aatgttgaac ccgcagaggc cggatatttg
     1441 aaggtaaata ctttagaaca tcatgttctt ccttggcaag gcacttattt tggtggcatg
     1501 gagaataaaa ttatcgcgta tgccaacgat cctgatattt gggcatttga tcattgggaa
     1561 gccggaacca caacattcag cacaacatgg gattctacca tggcaacact gacatctgat
     1621 gataatatca cagcagtctt ccgtcttaaa actgatatca atgataatga gcaaatggct
     1681 ttcgctgttt atccgacaat atttgaggat gcgttgatgt tgaattatac atccacagat
     1741 ggcagcgatg ttacatttac aatgtacgac acacatggtc gatttgtggc ttctaaaata
     1801 tttgccgctc caaccccagg cggatatgct gaatcctggg atgtatcctc attgcaatta
     1861 cctgcgggtt tatacctcat agaatgtgtt tccggagatc agaggggaag tgtgaaggtg
     1921 attaagcagt gattcctatg tggttgcgga ctgtttaaat aataaatatt gggatatttc
     1981 atatctcact tgttgacatg tgaaataaca atatatcaac tttcaccctt ttcaataaaa
     2041 aaattacaaa aaaattagga aatattgaaa taatttcaaa attttgatac cagaacctac
     2101 tttatgcagc tagatgacct ggattatcaa atattggaac aactcattca tgacggcaga
     2161 aagtcgttta ccgacattgc cgatgaacta agcgtatctg tcggcacagt gcgcaaccgt
     2221 tataacacct ttatcgaaaa cggattactc aacattattg gcagagtgca tcccgaaaag
     2281 atcggcttta atacctatgc acagattctt ataaaggtgc gtcccgttga aaagatttct
     2341 gctgtggcag ataatatttc acaactgcct gaagtgtcat ttcttgccat gaccacaggt
     2401 gcctacgacc tggaggtgaa tgtgatgtgt cgtaataatg aacatctcgt agaattgatg
     2461 acaaaccata tcactaaaat ggatggcatc ttcgaaagtt cgacgaatat ttatttcaaa
     2521 gtatataaat acgcacaacc tgatttgtat ctcttaagga gagagggtag tgctattgat
     2581 ataaataatg aaaaataaga acgcgaattt caacggatta aaaaacatac aaacccaaac
     2641 cctaatcatg cataaaccca ctacccaatt caaatttgta ttggtcattg cctgctggtg
     2701 tattgcgttt caattgcatg cgcagaccat tatcaccggc gtagtgcgtg attcctctac
     2761 aaatgaaaca ctcatcggag caaacgtgag actcgtttca gatccttcag tcggtacatc
     2821 cactgatctt gacggagcat tcacgctttc tctgcaagcg tcttccggtg cattgaaaat
     2881 atcgtttgtc ggatataatg aacaaacaat ttctttcaat ggttctggta cattgaatgt
     2941 actattatcg tcggaacata ttctcgagga aatcgtagtt gtcggatatg gcacacagaa
     3001 aaaaagtgat gtcaccggat ctatttcttc tacccgcaat gaggatttcc gcgatcagcc
     3061 aatttcaaat gtagccggaa gtatccaagg aaaagtaagc ggaattgata ttcgcaacgc
     3121 ttccggaacg ccgggagcag gattgctggt gagtgtacgc ggtcaataca atccgctgta
     3181 tgttgtcgat ggaattccga tgataagcga aagtaattca agtatttcta cttcatttga
     3241 tttacaggga aacagcgtag gcgcaggaca gaatatttca tcgatatcag atattaatcc
     3301 cgacgatatt gaatctattg aaatcttaaa agacgcatct gctgcatcaa tttacggtgc
     3361 ccgtgctgca aatggtgtaa ttcttatcac caccaaacgc ggagccgcag ggaaaacaag
     3421 attcagcctc aattattatt cgggaataca gcaggttagc agaccgctgg attttttgag
     3481 cagcgaagaa tttgtcgacc tcatcaatga agcgcgtcaa aacgacctca atgcctacga
     3541 agcagaccca tccttgtttg gcgatgatta tgaccccgcc ttgtcgacca accctttacc
     3601 agactcatgg gcaaccggtg taaacacaaa ttggatggac gagatattta gagtagcacc
     3661 cattaataat gttgaatttt ctgcatcagg aggaaatgat aaaacgaaat tctatatttc
     3721 aaactcctat ttcgatcaac aaggtattgt tatcgaatca tattataagc gatttaactc
     3781 gcgcatcaat ctagatcatc aagtaagtga taaacttaaa ataggtgaaa atatttcact
     3841 taccacttcc ggaaatcgac gttcactgaa tgataacgta tataccggaa cagtaactaa
     3901 tgctataggc gcatcaccgt tgatgccggt gtatgaagat gatggcagct atgcaagttt
     3961 tgaagattat caggctagct ggttgagtga caatcctgtg ctttctacaa aagaaatcat
     4021 tcctttcaca cgcaccaatc gcgcacttgc ctccatcttt gcagattatg ccttcacccc
     4081 aaaattacat ttcagatcta catggtcggc agatgtcact tttttgactg acgatattta
     4141 tttctctcca attaccaccg atgcggaagc agtaggcggt aaagccatga atagcgcttt
     4201 caataataaa gtttggctcg gtgaaaatat attatcttat aactccgaat taggtaacaa
     4261 taattttctc gatatcatcg gaggatttac attgcaagta tctaacagtg attatgtaag
     4321 tatcagcgga caaggatttc ctgcgggaag tggtttgcag gatgtatcaa gcgcagcaat
     4381 cgtcacaggt ggagaatcat tctcaaccgg ctggtcgctg gtgtcatttt tgggaagagc
     4441 gaattacacg attaaaagtc gctacttatt ttcactcagc gcacgtgttg acggttcatc
     4501 gcgcttttca ccaaataatc aatttggatt tttccccgca ggatctgtcg gttggcatat
     4561 gagcgatgaa aaattctggc cttcaggatt gtttatcacc gatatgaaac tccgcatgag
     4621 ctacggtatt accggcgatc aggaaattgg cgacttccaa tatatttctt tctggtcacc
     4681 tgtgggatat aacggacaag caggattagg tcctcgcaat cttgctgatg aaaatctgcg
     4741 gtggcaatcc aataaaatgt ttaataccgg tctcgactac gaattatatc gcggtagaat
     4801 ttccggctct ttggaatatt atatcggcaa taaaacagat ttattgagtg atgatgtaat
     4861 ttcaggtgta accggctttg gttcagtgac caggaattat ggtaacattc aaacttcggg
     4921 atttgaattg tcgtcgaata tatatgttta cgaccgaaaa gattttgatt ggactgttgg
     4981 cgtgaatgta agttggttgc ataatgagat tatagatctt agcacagact caatattatt
     5041 gagcgcatat tccgatttaa ctgcaacaca tattttagct gaaggtcagg catatggaag
     5101 tttttgggga gtaccttatg aaggtgttga ccctgaaaca ggcgatccga tttatacaga
     5161 caccaacggc gatggtgtga ttgatgatgc cgatgctcaa attttaggga aggcaacgcc
     5221 tgattggttt ggtggattaa atacttcact gcgctacaaa aaatgggatg ctcaaattgc
     5281 tgctacattt acgttaggca atgatgtgta caatatgatc agatcaacct atcaaaccgg
     5341 tggatggagc gattatggtt gggatgaaga atattattta tatcaggtat atgccaataa
     5401 tacgcacgtg atcgacgaca gatggcagca acccggagat gttacggata tacctcgggc
     5461 atcattgtat ttctataatt tctatcaaaa ctcaggtcag gcaattgaaa gtggctcaca
     5521 tgcacgtatt cgtgaaataa gcataggata tacgataaag ccttcttcca taaaatcatt
     5581 tgaatcattg agaatttatg ctcaagtgca gaatgcttat gtattcacga aatatacagg
     5641 cttcgatcct gaagtaagct ctacgggtgg cgatcatccg gaaactgcag gtgtcgatta
     5701 tgccgcatat ccgcaaccac gcacctatat ggttggattt aatttcaaat tctaacgcaa
     5761 aagcattgat catgaaaaag attttttatt tcatcatagc cgcaatggca ttaacaggat
     5821 ttaattcctg tgagggtttt ttgtcgcaaa cttcaactaa cgaagttgcc gatagtgatg
     5881 tgtttaccga tgctgcagga ttggaaaacg cgcgtatcgg tttgtataat tgtttacaaa
     5941 gccaatatta ttacggtggt tattatcaat tgatagccga tgcctatagc gataatggca
     6001 ccacaggtgg atatgataac ctcgatctcg atgaattagg gtataaggag gcttctgccg
     6061 ataatattta tatcgaaaat gtatggatag caatttacaa tactatttat actgccaatc
     6121 agattctcgc caatattgat aacatccagg atgaaacact tagcgatgaa gtaagtgacg
     6181 acatcaaagg agaagctttg gcgataagag caatggctca cttcgattta ttgcgcatgt
     6241 tcggcgaaca ttgggatttg tcgtcaagca tcggtattcc tgtggtgaca gaggttttat
     6301 ctacagacgc tcaaatgaaa cgaaatactg tttcagaaac atacaatgct attcttgccg
     6361 atttgatcac tgctgaatct atgctcaaaa acgacgacta tcgctacgat cctcctattt
     6421 ttaaaggtcc gcaattcatg acgcaaacgg cttgtcaggc attactggca cgtgtttaca
     6481 gaagcatggg cgatatgagc aatgcacttg ataaagcttc ggaggtaatt aacaccggat
     6541 ttgcttccat gatagacggc gatcaggttt ccgatattta taatacaaaa ttctcttcag
     6601 aagctatttt tgaattggca tttaacgctc aagacaaaag tcaatttaat gcgcttacct
     6661 atgcaagaga ggatgccctc cgcagtgagg tctctttttt agctgccgct gatcttaacg
     6721 atttttttgc aggaagacct gatgatttcc gggcaccatt gttgaatttt accggtaatg
     6781 acgttagtat tgaacccgat ggaagaactg aaaaatatcg cggagaaatt tttcaggata
     6841 atagcgcctt tattatacgt gctgctgaaa tgtacctcat tatgatcgag gcgaaaggat
     6901 atcccgccgg tgttactgat atgataagtt tcaccttgaa tcgcggtgta acgtcggtcg
     6961 acctaacttc ctctgaagca ttcgaacaag cattaatgaa tgaacgtcgg gcggaattaa
     7021 atttcgaggg cgacagatat ttcgacctgg cacattttgg aaaagtatct gagatattag
     7081 gtgatgaagt attgccttgt ttgccaattc caaacagaga aattggtgca aatggcggac
     7141 tgcttgaaca atatcccgga tactgatcgt taacattaat aattaaaaaa cattgaccaa
     7201 tagttttcga agtatacatc cttttgatca gcatgtgatt gctgaatatc cactcatgga
     7261 ggcaaaagcg gtggatactg ttttgatgca cagcgctgat gcttttaaac attggcggcg
     7321 cacttcaatg caattgcgtg ccgatttatt gttgcgcctt gcagttgtgt tgcgtgaaaa
     7381 caaagatgtt tacgcgaaaa taatttccct cgagatgggg aagctattta aagaagcaca
     7441 aggggaagta gaaaagtgtg catggaattg tgtgtactat gcagaaaatg gtgccgcaat
     7501 gattaatcct gaacctgcac cggcagatgg tagcgaaagc tatgtagtat tcgaacctat
     7561 aggtgcagtt tttgccatca tgccatggaa ttttccattt tggcaggtat ttcgttttgc
     7621 agcaccggta ttgcttggtg gaaataccat tatattaaag cacgcaccaa atgttacagg
     7681 ttgcgctatc gccatgcaac aaatattttt ggatgccgga ttccctgagc atgtttttca
     7741 aaccgtggta atagatgttg atcttgtaga aaacatcata gcacatgaca tcgttcaagg
     7801 cgtt
//
LOCUS       DAOQHY010000145         7734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_4905_length_7734_cov_4.669749, whole genome shotgun sequence.
ACCESSION   DAOQHY010000145 DAOQHY010000000
VERSION     DAOQHY010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7734
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4905_length_7734_cov_4.669749"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..>1150
                     /locus_tag="PLL28_11235"
     CDS             235..>1150
                     /locus_tag="PLL28_11235"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNF69940.1"
                     /translation="MPSDIVVEKKFHFTGHTAGVFALEGGLKPDTVLSGGGDGVIAEW
                     KLDGNRDAVGLARVPGNIFALLLMSDKGLVMAGDLYGGVYVIDTATRQEVNRFSWDGA
                     AVYAFLRLDDDRVAIGSGTGNLIIRNINSHTTEQVINIGQEAIRSLVKHPTLPQIALG
                     CSDNCVHILDADTLKTITVLHGHVNSVFSVQYHPNGKHLLSGARDAQIRIWDVVDGYS
                     CLQTLPAHMFTVNDIKFHPDGHLFASAGRDKHVKIWDAKTYALLKVIDKEKYDGHINS
                     VNKLFWSKWQNTLISCGDDRTVMGWGLIS"
     assembly_gap    1151..1250
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1603..2139
                     /gene="dcd"
                     /locus_tag="PLL28_11240"
     CDS             1603..2139
                     /gene="dcd"
                     /locus_tag="PLL28_11240"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNF69941.1"
                     /translation="MILTDKEIQSAIAKGDILIEPYRADCLGTNSYDVHLGRYLAVYK
                     NHILDAKKHNEIDEIIIPDEGFVIHPGILYLGVTEEYTETHAHVPFLEGKSSVGRLGI
                     DIHATAGKGDVGFCNTWTLEISCAQPVRIYAGMPIGQLIYFEVKGDITNYYNKKENAK
                     YVNRTIRPVESMMWKNKF"
     gene            complement(2250..2606)
                     /locus_tag="PLL28_11245"
     CDS             complement(2250..2606)
                     /locus_tag="PLL28_11245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNF69942.1"
                     /translation="MAYINRKNEVLAMSFDFSKALVYYCKQIEETGHKHIAWQLLRSG
                     TSVCANIFEAQTPESKSDFIHKLKIAAKECQETIYWLLLINETIESDKSEVYLKTAHS
                     IARLLSTIIASTRKSL"
     gene            3239..4525
                     /locus_tag="PLL28_11250"
     CDS             3239..4525
                     /locus_tag="PLL28_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNF69943.1"
                     /translation="MSFDKLGLIAPILKALQQEGYENPTPIQEQSIPAAIQGKDILGC
                     AQTGTGKTAAFAIPVLQRLHAANNKHPRAIRALILTPTRELAIQIGESFEAYGKYVGL
                     YHTVIFGGVSQHAQTQQLKRGIDILVATPGRLLDLMQQGFISLQHIETFVLDEADRML
                     DMGFIHDVKKVIAKLPAKKQTLFFSATMPKEITALADSLLHHPVKVAVTPVSSTVDLI
                     EQSVYFVSKGNKKQLLHHILKTKDIKSCLVFTRTKHGANKVCKDLVRAGFTAEAIHGN
                     KSQNSRQSALTNFKSGKTQVLVATDIAARGIDVEELTHVINYELPNIPETYVHRIGRT
                     GRAGSSGVAIAFCDEEERAYLRDIQKLIGKTILVHIDHPEYKKEIATPDAPLHAQRNR
                     PRTEKPKQQQTASAGGQKPRSKKPFWKNKKKQSKTA"
     gene            complement(4586..5107)
                     /locus_tag="PLL28_11255"
     CDS             complement(4586..5107)
                     /locus_tag="PLL28_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69944.1"
                     /translation="METLRVAFVLIACTISSAVNAQWSAEAQLAGSNFLGASLNLNYS
                     LSISANGDHKLTPTIGLGLSSNEWMPTSRMIIHAGLNYSYKRYGIGVEASQFGPLFPK
                     DGVAYDNELIVFPNINYLIVDRDMWYLKASLGAYFAFSELDGIDDKPTPTLSFEGDVI
                     PGAGLSVGIKLFK"
     gene            complement(5210..6688)
                     /locus_tag="PLL28_11260"
     CDS             complement(5210..6688)
                     /locus_tag="PLL28_11260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69945.1"
                     /translation="MRKLLFTSAFVCLHIAFLHAQDWSLFPADNHSYYRNNDMIYTVT
                     PDSNYVSGDTVFTLFQFSDTPLSASGCTNEIEDYLYAGGSLFLFPYVDYLAKVGDLTV
                     ITFGSGEYQMRFNTMCAKDDTFHLGGNLDPDLYIICNDVRIDNIFGTLDSVREFQVLH
                     DSGYDISLENTFILSKTFGLLSFYDFNQLPYYAIASMQYFEMIGIEGPIGDKGFKPPF
                     WKDYFPLQVSDVLIWDRHIYSHIPMYYIDYHLYYKDSVIARMDYPDSIVFYFDRWQKD
                     TSGIITQLPTYRLRHKRSELINMLEGPTGRFKHIEVTPGCYYFQHNILKSDGLKMLFS
                     GDDTIYSYQYFGGVYEIYDGCFVGETYDVGKSFTLQTDHGLVAGSYSVGDGNDYIVLA
                     GGIINGESFGIVDFETVDIANDLIPTFSLFPNPASEAVNIVLPDNVSGDIRIFNTAGN
                     CIVHAVSNIGKLTLDTSNWPSGIYMVQVVTNADSSSQRLMKF"
     gene            complement(6699..>7734)
                     /locus_tag="PLL28_11265"
     CDS             complement(6699..>7734)
                     /locus_tag="PLL28_11265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HNF69946.1"
                     /translation="NAGDVNGDGINDILIGADKYDAPSSDEGIAMLFLSCSTQFFADA
                     DGDGYGDDATLVNSCNAPLGYVSIGGDCDDDNASVHPGATEICNGIDDNCNGNIDEGF
                     ELSITIAALGATTFCEPGTVTLSASYTGTSIQWKRNGTNIAGATSATYTANQSGTYRA
                     ETSNACGSDLSEGIVVTVNKKPTATITASGATTFCAGGSVTLNVTPVAGCSYQWYNGA
                     TAIGGATGTAYVATTAGSYKCRVTKTATGCYKNSNIIDVTVPCKESLNSELGFSVFPN
                     PANEQLNVTVSEEIDGVVHITDLAGNIVIATEMHTGNMAIDISILPAGMYMLFILQES
                     KQLETKFIKQ"
BASE COUNT         2294 a         1629 c         1610 g         2101 t
ORIGIN      
        1 gctccctctc tatcgacggt aggagatgga gagtgcgggg ggtgaggcgg gtttcgcgca
       61 aagacgccaa gaggcaaagc cgcaaagaaa ttcaccattc acagctcacc actcaccact
      121 cacccactga cccactcacc cactcaccaa ttcccacatg gagcgcagcg acatcccgcc
      181 ttagcgggat tccctctttt cccttttctt tgcgtttgat ttagaaatat gttgatgcct
      241 tctgatattg ttgtagagaa gaaatttcat tttaccggtc acactgccgg ggtgtttgct
      301 ttggagggcg gattgaagcc tgatacggtt ctgagcggtg gaggcgatgg cgtgatagcg
      361 gagtggaaac tggacggcaa tcgtgatgct gtaggcttgg cgagggttcc ggggaatatc
      421 tttgccctat tgctcatgtc tgataaaggg ctggtgatgg ccggcgacct ctatggtggt
      481 gtgtatgtta tagacactgc aacccggcaa gaagttaacc gattttcttg ggatggcgct
      541 gcggtttatg catttctgcg acttgatgat gaccgcgtgg cgattggctc aggtacgggt
      601 aatctcatca ttcgaaatat caattcgcat acaacggaac aagtgattaa tattggtcag
      661 gaggccattc gttcgctggt taaacaccct acccttccac aaatcgcctt gggatgcagc
      721 gataattgtg tacacatttt ggatgcagat acattgaaga caattaccgt actgcatgga
      781 catgtaaact ctgtcttctc agtgcaatat cacccaaacg gaaaacacct gctatcgggc
      841 gctcgcgatg cacagatcag aatatgggat gtggtggatg gctatagctg cctgcaaacc
      901 cttccggcac atatgtttac cgtgaatgat atcaaatttc atcctgatgg tcatttgttt
      961 gcttccgctg gtcgcgataa gcatgtgaag atctgggatg cgaaaactta tgctttactg
     1021 aaagtgatcg acaaagaaaa atatgatgga catatcaact ccgtgaataa attgttttgg
     1081 agcaagtggc agaacaccct aatttcgtgc ggcgatgata gaacggtgat gggatggggg
     1141 ttaattagct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn accctaattt
     1261 cgtgcggcga tgatagaacg gtgatgggat gggggttaat tagctaatta gccaattagc
     1321 agattagccg attagccgat tgaatgcagg ggggtggaag tgtgggaatg tgagaatccc
     1381 gctgaggcgg gatgtcgctg cgctccatgt gggaatgcgt gcctcgctgc aggcgggtgt
     1441 gaatgtcccg ctagggcggg aggtcgctgc gctccatgtg caggtgcgga agttaatttg
     1501 gagtatagtc aacccacagg ctcacgagtc accactcacg aatcacgatt cacgattcac
     1561 gagtcacgag tcacgaaaga agaattaaat aatgaaacaa caatgatact tactgacaaa
     1621 gagatccaga gtgcaattgc taagggcgat attttgattg agccatatag ggctgattgc
     1681 ttgggcacga attcgtatga tgtgcatttg ggcaggtacc ttgccgttta caaaaatcac
     1741 atcctcgacg cgaaaaaaca caatgagatt gatgagatca tcattccgga tgaaggcttt
     1801 gttattcatc cgggcatcct atatctcgga gtaacagaag agtatactga aacacatgcg
     1861 catgttccat ttttggaagg gaaatcgagt gttggccgat taggtatcga catccacgct
     1921 actgcgggta aaggagatgt tggtttctgc aacacctgga ctctcgagat ctcatgcgca
     1981 caacctgttc gcatttacgc aggtatgcca atcggacaat tgatttactt cgaagtgaaa
     2041 ggtgatatca ccaactacta taacaaaaaa gaaaatgcga aatacgtaaa tcgcactatt
     2101 cgtccggtgg agagtatgat gtggaagaat aagttttgat gggctgatga ggtgatgtgc
     2161 tgatgggctg atgggctgat tggctaattg gcagatgagg ggggatgggc tgatgtaatg
     2221 atgggggggg gtgatgaggt tgtggaggat tagagtgatt ttcttgtgga ggcaatgatt
     2281 gtggatagga ggcgtgcaat tgagtgggct gttttaagat agacttcaga cttatccgac
     2341 tctattgttt cgttgataag tagcaaccaa taaatagttt cctgacattc ttttgctgca
     2401 atttttaatt tgtgaataaa atccgatttg ctttcgggtg tttgcgcctc gaaaatattc
     2461 gcacaaactg aagtaccaga gcgtagtaat tgccaagcga tatgcttgtg ccccgtctct
     2521 tcaatttgct tacagtaata aaccaatgcc tttgaaaaat cgaaggacat agctaatact
     2581 tcgtttttcc tattgatata tgccattatt atgcaccaaa ataaataatg tttgattttg
     2641 gcaatcaata aatatcaaag tttgtaaccg cttatactaa ttcaatttta attaaaataa
     2701 aagataaccg cttatgtttt gcccccattg taactcccat tgatttgaat aaatactgat
     2761 tttaacattt cgaataaatc cctcttactt cttgaatcaa aataagtatg catcccctac
     2821 tatcttgaac caaccatcaa caaaccacaa tataaattaa aaaccaatca tctgatcagt
     2881 aagttttaca tttccccctc atcataacaa cttccacatc actcgcatca cctcatcaac
     2941 ccatcctcca aatccacaaa atcatctcat cacttcatca gcacatcatc tcatcacctc
     3001 atcagcccat cacctcatca gcccatcagc acatcaccat ttcttcaatt ttttccctat
     3061 ctttgcctcg cgttcccgtt ttggttatcg gaagagtgtt cagatgaaaa caaggtttaa
     3121 acgggtgaac agcattcatt tttatccata aggcagacgt tataactgcg ccgaacacag
     3181 gtaggactta gtccttcact cgttgttaga tttccatcct tatacattct aactatttat
     3241 gtcatttgac aaattagggc ttattgcgcc tatattgaag gcgttacaac aagaggggta
     3301 tgaaaatcct accccaattc aggaacaatc cattcctgcg gctattcagg gaaaagacat
     3361 ccttggttgc gcgcaaacag gcacaggtaa aactgcagcc tttgctattc cggtgttaca
     3421 gcgattgcat gcggccaaca acaagcatcc tcgcgcaatt cgtgcactga tcctcactcc
     3481 tacccgtgaa ctcgccatac agatcggtga gagctttgag gcgtatggta aatatgtggg
     3541 actgtatcac accgttatct tcggtggtgt ttcacagcat gcacagacgc agcaacttaa
     3601 acgcggcatc gatattctgg ttgccactcc cggtcgcttg ttggatctca tgcagcaggg
     3661 atttatttct ttgcagcata ttgaaacatt tgtgcttgat gaagcagatc gcatgttgga
     3721 catgggattt attcatgatg tgaaaaaagt aatcgcgaag ttgcctgcga aaaagcagac
     3781 attattcttt tcagcaacga tgccgaaaga gattactgcg cttgccgact cattgctgca
     3841 tcaccccgta aaagttgcgg taacacctgt ttcatcgaca gttgacttga ttgagcaatc
     3901 ggtgtatttt gtgagcaagg gaaataaaaa acaattattg catcatatcc tgaaaacgaa
     3961 ggatataaaa agttgcctgg tattcacacg cacaaagcac ggtgccaaca aagtttgcaa
     4021 agatcttgtg cgtgcaggat ttactgcaga agcgattcat ggaaataaat cacagaatag
     4081 tcgccagagc gcattaacca atttcaaatc gggtaaaacg caggtattgg tagctaccga
     4141 tattgcagcg cgcggaattg atgtggaaga gttgacacat gtgatcaatt atgagttgcc
     4201 taatattcca gagacttatg ttcaccgcat cggtagaaca ggtcgcgcgg gaagcagtgg
     4261 tgttgccatt gcattctgtg atgaggaaga aagagcatat ctgcgcgata ttcaaaaatt
     4321 gatcggcaag acaattctcg tgcatatcga tcatcctgaa tataaaaagg agattgcaac
     4381 tccggatgcg ccattgcatg cacagcgcaa tcgacctcgc actgaaaaac cgaagcagca
     4441 gcaaaccgcg tctgcaggtg gtcaaaaacc cagatcgaaa aaacctttct ggaagaataa
     4501 aaagaaacag tcgaaaacgg cttaatacaa aaaaggtatt gttgtgatga caataccttt
     4561 tttttgcaga tcatttttca caaggtcatt taaataattt gatccccaca cttaaaccgg
     4621 cgccggggat gacatcgcct tcgaaagaaa gtgtaggtgt aggtttatca tcgatgccat
     4681 ccaattcgct gaaggcaaaa tatgccccaa gcgaagcctt gagataccac atatcgcgat
     4741 caacgattaa ataattgatg tttggaaaaa cgatgagctc attatcatat gcaacgccat
     4801 cttttggaaa taatggcccg aattgggaag cctcaacacc aatgccatat cgcttgtagg
     4861 aataattcaa tcctgcatgg atgatcatgc gcgatgtcgg catccattca ttacttgaaa
     4921 gcccgaggcc aatggttggt gtaagcttat gatcaccatt ggcagagatc gacaaactgt
     4981 aattgagatt cagtgaggca ccaagaaagt ttgaacctgc caattgagct tcggcactcc
     5041 attgtgcatt gacggctgaa gaaattgtgc aggcaattaa aacgaaagca acacgcagtg
     5101 tttccatgtc agttagtttt ggagtgaact aaaaacatat aacgaacgaa aaatctgaat
     5161 gttacaaccg ctcatgatct ttaacatata tcaatacaaa acggtatatc taaaatttca
     5221 tcaatcgctg ggatgatgag tcggcatttg ttacaacttg aaccatgtaa attcctgaag
     5281 gccaattact tgtatccaat gttagcttcc caatattact caccgcatga acaatgcaat
     5341 tgcctgccgt attaaatatt cggatatcac cactcacatt gtcaggcagt acaatattaa
     5401 cagcttcgga agccggatta ggaaataaag aaaaagtagg gattaagtca tttgcaatat
     5461 cgactgtttc aaaatcaaca attccaaacg actcaccatt aataatgcca cctgcaagaa
     5521 caatataatc attgccatcg cctacactat atgaaccggc aacaagtcca tgatctgttt
     5581 gtaaagtaaa tgatttgcca acatcatatg tttctccaac aaaacaaccg tcataaattt
     5641 cgtagacacc tccaaaatac tgataggagt aaattgtatc atcacctgag aacaacattt
     5701 ttaatccatc ggatttcaaa atattatgtt gaaaataata gcatccgggt gtcacttcga
     5761 tatgtttaaa tcggccggtt ggaccttcca acatgttgat taattctgac cgcttatgcc
     5821 ttaaacgata agttggcaat tgtgttatga taccggaagt atccttctgc caacgatcga
     5881 agtaaaacac aatactatcc gggtaatcca ttctcgcaat aacagaatct ttataataca
     5941 ggtgataatc aatatagtac ataggaatat ggctataaat atgcctatcc catatcagca
     6001 catctgaaac ctgcagtgga aaataatcct tccagaatgg aggcttaaat cctttatctc
     6061 cgatgggtcc ttcaatccca atcatttcaa agtactgcat acttgcaata gcataatatg
     6121 gtaattggtt gaaatcataa aatgaaagca atccaaaggt cttgcttaga ataaaggtgt
     6181 tttcaagaga tatatcgtaa ccactatcat gtaacacctg aaattcacga acgctatcta
     6241 aagtgccgaa aatattgtca attcgcacat cattacaaat tatatagaga tccggatcga
     6301 ggttaccgcc tagatgaaaa gtatcatctt tagcacacat tgtattaaac cgcatctgat
     6361 attcaccgct accaaaagta attacagtca agtctccaac tttagcaaga taatcaacat
     6421 acggaaacag gaaaagcgag cctccggcat atagataatc ctcgatttca tttgtgcatc
     6481 cacttgctga taaaggtgta tcactaaatt ggaaaagcgt gaagacagta tcccctgaca
     6541 catagttgga atcaggtgta accgtgtaga tcatatcatt attccgataa tacgaatgat
     6601 tatcagcagg aaataaactc caatcttgtg catgcaaaaa agcaatatgc aagcacacaa
     6661 atgctgaggt aaagagtagt tttctcatag tagaatattt actgcttgat gaatttagtt
     6721 tcaagctgct tactttcttg cagtataaac aacatataca tacctgccgg caaaatcgaa
     6781 atatcgatag ccatattgcc ggtatgcatt tctgttgcga tgacaatatt acctgcgagg
     6841 tctgtgatgt gcacaacgcc atctatctcc tcagaaacag taacatttaa ttgttcattt
     6901 gccggatttg gaaaaacact aaaacctaac tcactattaa ggctttcctt acaaggaact
     6961 gtgacatcaa tgatatttga atttttatag cagccagttg ctgttttggt tacacgacat
     7021 ttgtaacttc ccgcagttgt tgccacatat gccgttcctg ttgcaccgcc aattgcggta
     7081 gcgccattat accactgata gctacaaccg gcaaccggcg taacgttcaa tgtaacagaa
     7141 cctcccgcgc agaatgttgt tgcgcctgac gctgtaattg tggctgtagg ctttttgttt
     7201 acagttacaa caattccttc cgacaaatca gagccacatg cattggatgt ttctgcgcga
     7261 taagtgccac tttgatttgc agtatatgtt gcggacgttg ctcctgctat atttgttcca
     7321 ttgcgtttcc attgaatact tgtaccggta tatgaagccg ataaagtgac cgttcccggt
     7381 tcacaaaatg tagtagcacc caaagcagca atagtaatag acaattcaaa cccttcatca
     7441 atatttccat tacaattatc gtcaatacca ttgcatatct cagttgctcc cggatgaaca
     7501 gatgcattgt catcatcgca atcgcctccg atagatacat atccaagagg agcattacat
     7561 gaattcacaa gcgtagcatc atcgccatag ccatctccat cggcgtctgc aaaaaattgt
     7621 gttgagcatg acaggaacaa cattgcaatt ccttcatctg aagacggagc gtcatattta
     7681 tcggcaccaa ttaaaatatc atttatacca tctccattca catcgcctgc attt
//
LOCUS       DAOQHY010000146         7663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_4975_length_7663_cov_5.768927, whole genome shotgun sequence.
ACCESSION   DAOQHY010000146 DAOQHY010000000
VERSION     DAOQHY010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7663
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4975_length_7663_cov_5.768927"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="PLL28_11270"
     CDS             <1..799
                     /locus_tag="PLL28_11270"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNF69947.1"
                     /translation="LIDIDKIKSKFGNLMMTAAMIDDFIGWMLFSLVVTVANLESESS
                     FQGWQVMFMVIGFATFILTVGRRIIHWMFIFSNRYLSRGGGTLSVGIILCIFGAVFTE
                     WLGLHAIFGAFLVGIAVGDSEHFSHKNREMLHDIVTYIFAPLFFVSIGFRVDFIKNFD
                     GGVIAFVLLIAILGKMVGGYIGGRLGKFSHNESIAIGFGMNARGSQEIVLGLIALNAN
                     LITDQIFVALVVMTFVTIMIAGPSIRYFLQQHQEDDDEDEHNLTTAE"
     gene            864..1361
                     /locus_tag="PLL28_11275"
     CDS             864..1361
                     /locus_tag="PLL28_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69948.1"
                     /translation="MKGIMSAFLIVVGSLQAGATEVLEDTSFQRTNPKLLYTTRDYFV
                     NIPDNCMSDELCVKDFSFILTFNVGINTAQEIKQIGFKEADLDFMGKTAAAQTYKPHT
                     FTKYTSSSTNNGYSFTIDFIPDKAEGKMPVIEKFVFSGTITSGNLSGIMRFQTKNGVV
                     EVPLN"
     gene            complement(1358..2593)
                     /gene="rocD"
                     /locus_tag="PLL28_11280"
     CDS             complement(1358..2593)
                     /gene="rocD"
                     /locus_tag="PLL28_11280"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HNF69949.1"
                     /translation="MTSNTIVSAKTQQYIDQESKFGAHNYHPIPVVIEKAAGVSVWDV
                     DGKHYFDFLSAYSAVNQGHCHPRIIAAMTEQAKKLTLTSRAFHNNLLGPFEAYMTELF
                     GYDKLLPMNSGAEAVETALKLCRRWGYEVKGIAEGKAKILVCEGNFHGRTTGIISFSN
                     DPDSTGGFGPYMPGYQIIPYNNLSALAEALRDPDVAGFLVEPIQGEAGVYVPDQGYLS
                     GAYAMCKDANVLFIGDEIQTGLARTGKMLACDHEDVRPDILILGKALSGGTMPVSAVL
                     ADDEIMLTIQPGQHGSTFGGNPLACAVAMTAMQVLVEEKLAENAEVMGAMLREALSGM
                     NSDYIKVVRGKGLLNAIVVNDNYNNKSAWDFCLALMENGLLAKPTHGDKIRFAPPLVI
                     TKEEMQTCIEIIRKTVNEF"
     gene            2845..3231
                     /locus_tag="PLL28_11285"
     CDS             2845..3231
                     /locus_tag="PLL28_11285"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNF69950.1"
                     /translation="MKKSHIILLVLLALCIGVFASKLGNVSSYSSYKEAKEKEGKSVQ
                     LVGNLNKEKPVNFDPVKDPNSFSFTLIDKNGDNVEVVCYDDMPTDFEKSEDIVLTGAM
                     KGEVFYANEMLVKCPSKYQDNEITKQ"
     gene            3240..5741
                     /gene="ccsA"
                     /locus_tag="PLL28_11290"
     CDS             3240..5741
                     /gene="ccsA"
                     /locus_tag="PLL28_11290"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNF69951.1"
                     /translation="MGESHQHAIQEVNPIFGQIGQVLIITAFVAALLATWYYFRATRS
                     QQQIGENDNRKIARAFFIGHALSIVGVVVTMFIMIFNHHFEYHYVWSHSSSDLQMKYI
                     LSCFWEGQEGSFLLWTFWHVVIGLVVMRTSKEWETPVMSIVGLMQVFLLTFVLGIYVG
                     SWHIGSSPFLLLKDKLTADPVFLFNDYMRFIPDGRGLNALLQNYWMVIHPPVLFCGFA
                     SITFPFAFALAGLWKGKYTEWIKPAMPYALFAGMVLGTGILMGGAWAYESLSFGGFWA
                     WDPVENASLIPWLIIVAGLHNMLIAKKTGQALRSTFVMIFLAFFFVLYSSFLTRSGIL
                     GDTSVHAFVEEGLTNHLLLFVGVFFFLSFIPLIRKWKHIPAPEREEKLNSREFWMFVG
                     SLVLLFGGLHVIFFTSIPVFNTLITHVNDILGTSIRNDLGTPDDPIHFYSGVQIWVGV
                     LIAMLSAFAQYLRYKNSDGAGFVKRISYSFIFAIAATVISSIMLDKLWHITSERGVFH
                     LITSYSILLFAAWFSVIANAQYFIQVLKGKIRVGGGSVAHIGFGILLLGILISNAGQR
                     VISLNTGDVRFDAQGMDDTFQRENVRLIKNVPYPITDYNVTYLGDTLIAGATYYDLLF
                     TKTDPETGELMQQFTVHPYLLMDTKMQQLAPNPDTKHYLTYDVFTHVTSLPGEGSKNA
                     PPTTTLDTIGVGDTLHFDTGYMILKDLKNTSFSADSNEVKAFFDVHAGIASESIEVPF
                     IVSGRTMQVLQGTANVSVANVQVSFITILPDQSKKFVFKVDDSSLNTDWIVIKSIIFP
                     MINLVWIGVIVMAFGFFMSMLARLRERKEKSVVNG"
     gene            5734..6516
                     /locus_tag="PLL28_11295"
     CDS             5734..6516
                     /locus_tag="PLL28_11295"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HNF69952.1"
                     /translation="MDEIKSILLVGTGNAAWHLGLNFKLNGVLIAGIVARDVEKGIAL
                     SAALGDVPVYGLEDKLPEVDATILLVRDDAVVEVAGKVAQEGKLLVHCSGSIKAEAIK
                     SGEHAFAVAWPLQTLQKDHPRPLFNTVVAVTGSDAETEQLVAGLFGKISNRVHVMSEE
                     QRSVMHMCAVWINNYTNHMYYIGHSLLKEKGLEFSMFFPIIEEHVQLLHEQDPEKLQT
                     GPAKRGDMGTLSRHMALLKDHPEWQDLYNQLSGSILKTYLKK"
     gene            6538..6867
                     /locus_tag="PLL28_11300"
     CDS             6538..6867
                     /locus_tag="PLL28_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNF69953.1"
                     /translation="MVTIKFTFEDGSEAKEVQVEEGYSVLEAALLNNIKLNHNCGGVC
                     ACSTCHVYIEKGMEALPETTEKEEDFIDRARDPRLNSRLSCQCDLMEDGTFIEVLVPD
                     QSTIIAS"
     gene            6885..7124
                     /gene="iscX"
                     /locus_tag="PLL28_11305"
     CDS             6885..7124
                     /gene="iscX"
                     /locus_tag="PLL28_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HNF69954.1"
                     /translation="MSQFEEPPIYWNDHEDIALKLYERFGDDFNESKIYRIRFTELIE
                     WVLEIPDFKGTREECNEGHLEMVQSAWVYEWRDNQ"
     gene            7179..7637
                     /locus_tag="PLL28_11310"
     CDS             7179..7637
                     /locus_tag="PLL28_11310"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNF69955.1"
                     /translation="MKDWIRIIRPVNLLLLAATLYAIDGFILQPNFNTYGIRFSLNEF
                     QYFLLVISIVLICAGGYILNDYFDVQIDEVNKPSKVMIGKGIAPNSAFYVYMLLSFGG
                     LAIGTYLSLAVGNWKLVTIYVMAIALLYFYSATFKRYPLIGNMMVAYWPA"
BASE COUNT         2157 a         1449 c         1691 g         2366 t
ORIGIN      
        1 acttatcgat atagataaga ttaaaagcaa attcggaaat ctcatgatga ccgctgccat
       61 gatcgacgat ttcatcggct ggatgctgtt ttcattagta gttaccgttg ctaatctgga
      121 gagcgaaagc agcttccaag gctggcaagt gatgtttatg gtgatagggt ttgccacatt
      181 tatcctgact gttggccgcc gcattatcca ttggatgttt atcttctcca atcgttacct
      241 ctcccgcgga ggtggcacgc tttcggtagg gattattttg tgcatcttcg gcgccgtgtt
      301 tacagaatgg ctgggacttc atgccatttt cggagctttc ctggttggta ttgccgttgg
      361 tgattcagaa catttcagcc acaagaatcg cgaaatgttg cacgatatcg tgacatatat
      421 tttcgctcca cttttcttcg tatcgattgg attcagagta gatttcatca aaaatttcga
      481 tggtggggta attgctttcg ttctgcttat tgccatctta ggcaaaatgg taggggggta
      541 tattggtgga cgattaggga aattctctca taatgaatcc attgccatcg gttttggcat
      601 gaatgcacgc ggatctcagg aaattgttct tggattaatt gcattaaatg ccaatcttat
      661 caccgatcag atatttgttg ccttagtggt gatgaccttt gtaactatca tgatagcagg
      721 tccttcgatt cgctatttcc tgcagcaaca tcaggaagat gatgatgaag atgaacacaa
      781 tttgacgaca gccgaatagc ttatccacat ttagccggaa atagaatggc acggtatttc
      841 gttatacatt gcaaagtcaa ggtatgaaag ggataatgag tgcattttta attgttgtcg
      901 gcagtctgca agcaggcgct accgaggttt tggaggatac cagcttccag agaaccaatc
      961 ccaaattgct ttacaccaca cgcgattatt tcgttaatat tccggacaat tgcatgagtg
     1021 atgaattatg tgtgaaagat ttttcattca tcctcacatt caatgtaggc attaatactg
     1081 cccaggaaat caaacaaatt ggttttaaag aagctgacct tgattttatg gggaaaacag
     1141 cagcagcgca gacttataaa ccacatacgt ttacaaaata tacgtcgtca agtaccaaca
     1201 acggatattc ttttaccatt gattttattc ccgataaagc agagggtaaa atgccggtga
     1261 tagaaaaatt cgtgttttcc ggtactatca catccggtaa tttatcaggt atcatgcggt
     1321 ttcaaacgaa aaacggtgtt gtagaggtcc ctctgaacta gaactcattt actgtttttc
     1381 gaatgatctc gatacaagtt tgcatttcct ctttggtaat taccaatgga ggagcgaatc
     1441 gaattttatc gccatgtgtt ggtttagcca acaatccgtt ttccattaag gccaggcaaa
     1501 aatcccatgc tgatttgttg ttgtaattat cattcaccac aatggcattc aataaacctt
     1561 ttcctcttac aactttgata taatcggaat tcattccact aagcgcttcg cgtaacatag
     1621 cacccattac ttcggcattt tccgcaagtt tctcttcaac aagcacttgc atagcagtca
     1681 tggcaacagc acatgcaagg ggatttcctc cgaacgttga tccatgctga cctggttgta
     1741 tcgtcaacat gatttcatca tccgccaaaa cagcacttac gggcatggtt ccaccactga
     1801 gggctttgcc cagaatgagg atatccggac gaacatcttc atgatcgcag gcgagcattt
     1861 ttcctgttct ggcaagtccg gtctggattt catcgcctat aaacaacaca tttgcgtcct
     1921 tgcacatggc ataagcgcct gacaaataac cttgatccgg aacatacaca ccggcctctc
     1981 cctgaattgg ctctaccaga aatcctgcaa catccggatc gcgcagtgct tcagcgagtg
     2041 ccgacaagtt attgtatgga atgatctgat aaccaggcat ataagggcca aaacctcccg
     2101 ttgagtccgg atcattgctg aatgatataa tgcctgtagt tcttccgtgg aaattaccct
     2161 cgcaaacgag aatttttgcc tttccttctg caataccttt cacctcatag ccccagcgac
     2221 gacaaagctt taaggcagtt tcaaccgctt ctgctcctga attcattggc aacagcttgt
     2281 catagccgaa taattcagtc atataagcct caaacgggcc aagtaaatta ttatgaaagg
     2341 ctctgctggt caatgtcaac ttcttcgcct gctcagtcat ggctgcaatg atgcgtggat
     2401 ggcagtggcc ttgatttaca gctgaataag ctgataggaa gtcgaaatag tgctttccgt
     2461 caacatccca aactgataca ccggctgctt tttcaattac aacaggtatt ggatggtaat
     2521 tatgcgctcc gaacttcgat tcctggtcaa tatattgctg ggttttggcc gatactatgg
     2581 tattagatgt catataatgt gacttaaagt attgattatc aaatagttat gaaaaatgaa
     2641 aaatagacat ggagatacag gaaaccgcta tcagacctgt ggatggtcga ggaaatcgag
     2701 gttcatatcc aaaaagtgca tcatatcgca acaaagatag ccaaaaaaag gatgtgagat
     2761 aatcgttcac aaatactgaa gtaatgccat gctaacagaa cattttgggc tacctttgcg
     2821 ttgaaaatta taaaggttca ggtaatgaaa aaatcgcata tcatattgtt ggtactcctt
     2881 gcgctttgta taggagtatt tgcctctaaa ctgggcaatg taagctcata cagcagttac
     2941 aaggaagcca aggaaaaaga gggtaaatca gtgcagttgg ttggcaacct gaacaaagag
     3001 aaacccgtaa attttgatcc tgttaaagat ccgaatagtt tctcattcac ccttatcgac
     3061 aaaaatggtg ataatgtaga agtggtttgt tatgacgaca tgccaacgga ttttgaaaaa
     3121 agtgaagata ttgtattgac cggagctatg aagggagagg tgttttatgc caatgaaatg
     3181 ttggtgaaat gtccttccaa atatcaggat aacgagatta cgaaacagta aacacctgta
     3241 tgggagaatc gcatcagcac gcaatacagg aagtcaaccc gattttcgga cagatcgggc
     3301 aagtattaat catcaccgca tttgtagcag cattactcgc aacctggtat tatttcaggg
     3361 caacgagatc gcaacagcag attggtgaga atgacaatcg caaaattgcc agagcatttt
     3421 ttatcggaca tgcactctca atagttggag tggtggttac catgttcatt atgatattca
     3481 atcatcactt tgaatatcat tatgtatggt cgcattccag cagtgatttg caaatgaaat
     3541 atatcctttc ctgtttttgg gaagggcagg agggtagctt tttattgtgg accttctggc
     3601 acgtagtgat aggcttagtt gtgatgcgaa cctcgaaaga gtgggaaacg cctgttatga
     3661 gtatcgtagg tttgatgcag gtatttttgc tcacattcgt cttaggcatt tatgtcggta
     3721 gctggcatat aggtagttcg ccatttttat tgttgaaaga caaactcact gccgatcctg
     3781 tatttctctt taatgattac atgcgtttca ttcctgacgg aagagggtta aacgctttac
     3841 tgcaaaatta ttggatggtg atacatccgc cggtattatt ttgtggattc gcatctatta
     3901 catttccatt tgcatttgca ctggcaggtt tgtggaaagg aaaatatact gaatggataa
     3961 aacctgcaat gccctatgct cttttcgcag gaatggtttt gggaacagga atattaatgg
     4021 gcggtgcctg ggcttatgaa tcattgagtt ttggcggctt ctgggcatgg gaccctgttg
     4081 aaaatgcatc gttgatccct tggttgatta ttgttgccgg attacataat atgctaattg
     4141 ccaaaaaaac cggacaggct ttgcgttcaa catttgtaat gatattcctc gcgtttttct
     4201 ttgtgcttta ttcatcattt ctcacacgca gcggaatcct tggcgacacc agcgtgcatg
     4261 catttgttga agaaggttta acgaatcact tgctattatt tgtcggggta tttttcttct
     4321 tgtcatttat tccattgatt cgcaaatgga aacatatccc ggctcctgaa cgtgaagaaa
     4381 aattgaactc cagagaattc tggatgtttg tcggatcgct ggttttattg tttggcggat
     4441 tacatgtcat cttctttaca agcattccgg ttttcaatac tttgattacg catgtaaatg
     4501 atattttagg tacgtctatc cgcaatgatc ttggtacacc tgacgacccg attcactttt
     4561 attcaggagt acaaatttgg gttggtgtat tgattgccat gctgagtgca ttcgcgcaat
     4621 atctcagata caaaaactct gatggtgcag gatttgtcaa acgtatttcc tattcattca
     4681 ttttcgccat agccgcaact gttatcagct ctatcatgct ggataaactg tggcatataa
     4741 catctgaaag gggtgtattt catctcatca cttcttattc aatattgctg tttgccgcat
     4801 ggtttagtgt gattgctaat gcgcagtatt ttattcaggt gcttaaagga aagataagag
     4861 taggaggggg ttccgttgcg catatcggtt tcggaatttt attactcggc atactgatct
     4921 ctaatgcagg tcaacgtgtc atttcactaa ataccggcga tgtccgtttc gatgctcaag
     4981 ggatggatga tacctttcag cgtgaaaatg tgaggctgat aaagaatgtg ccttatccca
     5041 tcacagatta taatgtaacc tatttaggag atacactgat tgcaggagca acttattatg
     5101 atttgttgtt cacaaaaaca gatccggaga caggtgaatt gatgcaacag tttacagttc
     5161 atccttattt gttgatggat actaaaatgc agcagttagc accaaatccg gatacgaaac
     5221 attatctcac atacgatgtg tttacacatg ttacctcact tccgggtgaa ggttcaaaaa
     5281 atgctccgcc tacaacaact ttagatacaa taggagttgg cgatacactt catttcgata
     5341 ccggatatat gattttgaag gatttgaaga ataccagttt ttcagcagat tcaaatgagg
     5401 tgaaggcatt ttttgatgtt catgccggta ttgcttctga atctatagaa gtgccattta
     5461 tcgtatcagg gcgcaccatg caagtattgc aaggaacagc aaatgtatca gttgccaatg
     5521 tgcaggtttc gttcattacc attttaccag atcaatcgaa aaagtttgtg tttaaggttg
     5581 atgacagttc actcaatact gattggattg ttataaaatc catcatattc ccaatgataa
     5641 atcttgtttg gattggtgtg attgtaatgg cattcggatt ttttatgagt atgctggctc
     5701 gacttcgcga acgcaaagaa aaatctgttg tcaatggatg aaattaaatc catcttgctc
     5761 gtaggcaccg gaaacgcagc ctggcatctt ggtttaaatt tcaagctgaa tggagtgtta
     5821 attgccggta ttgtagcgcg tgatgttgaa aaaggaattg ctttatctgc tgcattaggt
     5881 gatgttccgg tatatggttt ggaagacaag ctgccggaag ttgatgcaac aatattatta
     5941 gtgcgtgatg atgcagttgt agaagtggcg ggcaaagtgg cacaagaagg aaaattgctg
     6001 gtgcattgtt ccggttcaat aaaagcggag gctattaaat ccggtgaaca tgcatttgcc
     6061 gttgcctggc ctttgcaaac attgcaaaaa gatcatccgc gcccattgtt taataccgtt
     6121 gttgcggtaa ccggtagcga tgcggaaact gaacaactgg tagcaggact attcggtaag
     6181 atcagcaatc gcgtgcatgt gatgagtgaa gagcagcgat ctgtgatgca tatgtgtgcc
     6241 gtgtggatca ataattacac gaaccacatg tattacattg gtcattcttt gttgaaggaa
     6301 aaaggactgg aattttcgat gttttttcct atcatcgaag agcatgtgca attgttacat
     6361 gagcaagatc ctgaaaaact gcaaacaggg cctgcaaaac gcggcgatat gggaacttta
     6421 tcacgacata tggctttgtt aaaagatcat cccgaatggc aggatcttta caaccaattg
     6481 tcgggtagta ttttaaaaac gtatttaaag aaataatttt gtgttgaatt tcaggctatg
     6541 gtaacgatca aatttacttt tgaggatggc agcgaagcga aagaagtgca agtggaagag
     6601 ggttattccg tattggaagc agctttgctg aacaatatta aattaaatca caattgtggt
     6661 ggtgtttgcg catgcagcac ttgccatgtt tatattgaaa aaggcatgga ggcgcttcct
     6721 gaaactacgg aaaaggagga agacttcata gacagagctc gcgacccgcg attaaattcg
     6781 cgactcagtt gccaatgcga tctgatggaa gatggtacct ttattgaagt gctagttccg
     6841 gatcaaagta ctattattgc atcataatat atttattcag ctatatgtct caatttgaag
     6901 aaccacctat ttattggaat gatcatgaag atatcgcact gaaattgtac gagcgatttg
     6961 gtgatgattt caatgaatca aaaatttata gaattcgatt caccgaactc atcgagtggg
     7021 tgctggaaat tcctgatttt aaaggaacac gtgaggaatg taatgaaggc cacctcgaaa
     7081 tggttcaaag tgcatgggtg tatgaatggc gcgataatca ataacacttg tataccattt
     7141 tgttgcctgc tttcgttatc ctgtaaacct cattacctgt gaaagattgg atacgcatca
     7201 ttcgtcctgt taatttatta ttgctcgcag ctactctgta tgccatcgac gggtttattc
     7261 tgcaaccgaa tttcaatacc tatggaattc gtttttcatt gaatgaattt cagtatttct
     7321 tgctggttat ctctattgtc ttgatttgtg caggaggcta tatcctcaat gattatttcg
     7381 atgtgcagat tgatgaagtt aacaaaccct cgaaagtgat gatagggaag gggatagctc
     7441 caaattcagc cttttatgta tatatgttgc tcagctttgg tggtttggcc attggaacat
     7501 acctcagttt ggcagttggc aactggaagc ttgttacaat ttatgtgatg gcaattgcat
     7561 tactgtattt ttattctgca acctttaagc gatatccttt aatcggaaat atgatggttg
     7621 cttattggcc ggcataagca ttttgatgac atttatttat gaa
//
LOCUS       DAOQHY010000147         7614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_5021_length_7614_cov_6.542267, whole genome shotgun sequence.
ACCESSION   DAOQHY010000147 DAOQHY010000000
VERSION     DAOQHY010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7614
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5021_length_7614_cov_6.542267"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..703)
                     /locus_tag="PLL28_11315"
     CDS             complement(170..703)
                     /locus_tag="PLL28_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNF69956.1"
                     /translation="MQNPLILPVHGVMPEFGENCYIAPNATVVGQVKMGNDCSVWFNA
                     VVRGDVNTIVMGNKVNVQDNAVIHCTYKTAPTTIGNEVSIGHNAIVHGCTIHDHVLVG
                     MGAIVMDHAVVHENVLIAAGSVVLENSILESGFIYGGVPAKKIKPLSPEEFANTISRI
                     SNNYVMYAGWFSGENGD"
     gene            complement(713..1237)
                     /locus_tag="PLL28_11320"
     CDS             complement(713..1237)
                     /locus_tag="PLL28_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69957.1"
                     /translation="MQISPAHWHLLVNHFPIILVMTGALFFILGFIMKKPWLHNAALL
                     LTILAALAGFIANQTGEGAEESVEELPGISHQAIHIHEETAETGLIIILITGIVAVIS
                     TLVNMKNKKTGRIFLIITMLATFASSGYMGYVGLTGGEIRHSEIRGDFGRGNTANSNT
                     APAGGEEEAGEDDD"
     gene            complement(1308..2903)
                     /locus_tag="PLL28_11325"
     CDS             complement(1308..2903)
                     /locus_tag="PLL28_11325"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNF69958.1"
                     /translation="MNKLLFVNIGGVVFQIDDTAYRTLDQYLESIRKKYANTAEGEEI
                     IRDIENRIAELFLEKVGERSAITMQYVEEVIALMGAPEDFESASSEQSSTGQQQQYRQ
                     GPSTTRFFRDKEHNVLGGVCSGLAAKFDIDALWLRLAFLIAFFFAGTGLLLYIILWII
                     IPEAKTTAEKLEMRGEKIDIDNIERTVRDGAKQFSQRMNAFGEEVKSTFSSENMEKTR
                     RNTGDFIEKTVNTIAPVGKTIARVVAFGIMLVCMLLIVVVSIELISNWGRHFMDVNFL
                     GSHIMEGSNQAWLMVTCALALFFIPLLGIIVSMVKYLLGIKQRTRFISITLGVLWSIA
                     LFGAIYIAITIGRNFQYEASVNSKLPVVQPANSTMYLQLEMVDGNWPYRGHFDKHGPI
                     KKGDGGPFFEWYEQDSLAFKDISVAFERSVDSNYVVLVNKSARGYDNASAKETAQSFT
                     YNLQQFGDSVIHIPSLIKLQDNEQWREQSVEIIIRVPVNKYVQIDHRLENYLDYNEYI
                     SDLRTPQLFDNKLRMTTAGLQAY"
     gene            complement(2900..3244)
                     /locus_tag="PLL28_11330"
     CDS             complement(2900..3244)
                     /locus_tag="PLL28_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNF69959.1"
                     /translation="MNIENAQAQMRKGVLEFCILAIISEGEIYPTDVISRLKESNLLV
                     AEGTVYPLLNRLKTMELLSYKWVESNSGPPRKYYQLTDTGRKFLSELDKTWQELVHAV
                     SINTKNIKLTQQ"
     gene            3465..4001
                     /locus_tag="PLL28_11335"
     CDS             3465..4001
                     /locus_tag="PLL28_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HNF69960.1"
                     /translation="MIPPVQEAPLGMEENCGLGAEAWGLRGSEPTPCTGSEQVVMKDT
                     DFSPPIQITHATKKPDSATKYTHAGDKQQYVTLTKPEVQEQDSDVGETEERTLHPAAI
                     AGLILTILGGGILSPIGWVGIILSVVALKKIKTNPDIYYGKGLAITSMIYFIISIIAL
                     IALIIFAIYFSGIFLSIP"
     gene            complement(4005..5672)
                     /locus_tag="PLL28_11340"
     CDS             complement(4005..5672)
                     /locus_tag="PLL28_11340"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNF69961.1"
                     /translation="MRNILWRLCLLVMMLSIGNALLYAQTPFEYKYAQDVFNKLIRTI
                     NLSSPPKPILEVVPDPNMLATTSGNGKVTIGYQLVNKCRSFGKDSSNALAHVLSHELT
                     HYYQNHFWSEQFGSAYADVGWGEQMQQSGKSLNVMRLYETQADEYGMYYAFSAGYRTL
                     YIGDKVLDSIYTWFHLNEQLPGYPSLTERKQISIEARANIAKLIPVFETGNNCLLLAQ
                     MMGGDQQMLLAKTAALQFDDIIARKMQTKEMYNNAAIARLLSVIPMFPDSLQNLQYPF
                     MVETGSILYKSERSRSANGVTDPVMLDAMNTTIDEANTLLNNAVKLDKQFYPAWMNLA
                     ILYYLRGAYGSATDALQNASRSMGANNALLWSVDEMRAIIAAAQDDDAEATQLFSSAT
                     TKGSQTATYNYACVYRDASNGLIKIDNTLSAYATDTTEMLNGESIFHFMDNLPPNRAG
                     RFDVQNEELVLFTDTTSEYILTYVRANFKPAYSKLRFISSSENPAVKTSKGLRKGDSL
                     QQLIALYGKPSKIFLTGRGEVYAYPAQNIFVHISDEHVSEWTYWWLK"
     gene            complement(5678..>7614)
                     /locus_tag="PLL28_11345"
     CDS             complement(5678..>7614)
                     /locus_tag="PLL28_11345"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNF69962.1"
                     /translation="TFVAAVCLTVAANAQMGMGMVENPALHNTFRAKKPDAKGYGSEM
                     PKAWSLMAYTPYVKSQGQYGTCASWSSTYSAYTTAWAIKMGMTDRNLITALSFCPYFV
                     YNQVNGDANCSSGNSLEDVLVFMMDNGSKRFYMPVIGCGTGVTPEMTADASQYKINDA
                     YILYDVQDFPQERTAAAVTKFLTTKAKPDIDAIKSAIASNNPVVFGGFVANSFMQAMG
                     TDHWTPTYEERTNPGQAVLDNHGMHQLHAMTIIGYDDNKYGGAFQIMNSWSSLWGENG
                     YIWVSYDDWALFNYKAFWLDIGFVRLEILTKEGCASGDCNNGYGVMKWNNGQRYEGHF
                     VNGERSGYGIYTWPDGNAYAGQWKAGKREGEGVLYNPDGSYGTCMYANDVQVSGFGNW
                     TYNNGDRYQGVLNTGFVRNGYGVYTFTDGRVYEGAFKDESFSGLGKMTWPNGDSYMGE
                     WLDNNMHGYGIYTYANGKVKAGKWNFGLLGNGQSYGYAASDGHQANRIQGLFAAVDYA
                     TADCVTGDCLNGKGSKKYSSGLVYEGEFKDALEDGYGTWTFTDGSKQTGYFVQGTPAG
                     VFSVTFSGGDKMIGENVKGKVNGYGLYISSSGTMTLEYYSDGTFIREVTPSSTTMQTQ
                     LTPEKFGEKPHTDLDVNQSK"
BASE COUNT         2078 a         1748 c         1472 g         2316 t
ORIGIN      
        1 ctattaacgc gccagcgtta aaagcactcc acacttgcta atttgctaat tctcacactt
       61 gctaattaac cacacttgct aacttgctaa ccctcaaact tgcaaactaa aacgagcgtc
      121 ttcttgcaag cacactccca attagctaat ctgctaattc tcaaatttgc taatcaccat
      181 tttcccccga aaaccacccc gcatacatca cataattatt tgagatacgc gaaatcgtat
      241 tcgcaaattc ttccggactg agtggtttga ttttttttgc aggaacacca ccgtagataa
      301 agccactttc aagaatacta ttttctaaaa ccacactgcc agctgctatt aatacatttt
      361 cgtgcacaac agcgtgatcc attactatcg ctcccatgcc caccagcaca tgatcgtgga
      421 tggtgcagcc gtgaacgatt gcgttgtgac cgatagatac ttcattaccg atggttgttg
      481 gtgctgtttt gtaagtgcaa tgtataacag cattatcctg cacattcact ttatttccca
      541 ttactatggt atttacatca ccacgaacaa ccgcattaaa ccatacactg caatcgttgc
      601 ccatttttac ctgaccaact acggtggcat taggagcgat ataacaattc tcaccaaatt
      661 caggcatgac gccgtgcaca ggaagtatca aaggattctg catggtatat gtttaatcgt
      721 catcttcacc ggcttcttct tcaccacctg ccggggcagt atttgagttg gctgtatttc
      781 ctcgtccgaa atcgccgcga atttcgctgt gacgaatttc accgccggtc aatcccacat
      841 aacccatata acccgaactc gcgaaggttg ccagcatagt gatgatgaga aatatgcgac
      901 cggttttttt attcttcata tttacaagcg tgctgatcac tgcaacaata cctgtaatta
      961 gaataattat taatccggtt tcagctgttt cttcatgaat atgaattgcc tggtgtgaaa
     1021 ttccgggcag ctcttctaca gattcttccg caccttctcc ggtttgattg gcaatgaagc
     1081 cggcaagcgc cgctaatatg gttaataata acgcagcgtt gtgcagccat ggttttttca
     1141 tgataaaacc aaggataaaa aacaaggcac cggtcatcac taagatgatg ggaaaatgat
     1201 tcaccagaag gtgccaatgg gcaggactaa tttgcataat ttgatgttta aagttaaaac
     1261 taatttcctt ttctaaaaaa tgcctgccaa tagttgatgc ttgatggtta atatgcctgt
     1321 aagcctgcgg ttgtcatgcg cagcttgttg tcgaataatt gaggggttcg caaatctgaa
     1381 atgtattcgt tgtaatccaa ataattttcc aaacgatgat ctatttgtac atatttatta
     1441 acgggtacac gaataataat ttcaaccgac tgctcacgcc actgttcatt gtcttgcaat
     1501 ttgatcagcg atggaatatg tattacagaa tcaccaaatt gttgcaagtt atatgtaaat
     1561 gactgtgctg tttcttttgc cgaggcattg tcgtaaccgc gtgccgattt attaaccagc
     1621 accacataat tgctgtcgac actgcgctcg aatgccacac tgatatcttt aaatgctagg
     1681 ctatcttgct cataccattc gaaaaacgga ccgccatcgc cttttttgat gggaccatgc
     1741 ttgtcgaaat gtccgcggta tggccagttg ccatcaacca tttcgagttg aagatacatg
     1801 gttgaattag ccggttgcac aacaggcaat ttgctgttta cacttgcttc atattggaaa
     1861 ttgcgaccaa ttgtgatagc aatataaatt gcaccaaata atgcaatgct ccacagcaca
     1921 cccaatgtaa tgctgataaa tcttgttctt tgtttaatac caagcaaata tttcaccatc
     1981 gatacgatga ttcccaacag tgggatgaag aataatgcca atgcacatgt taccatcagc
     2041 caagcctgat ttgatccttc cataatatgg ctgccgagga aattcacatc cataaaatga
     2101 cgtccccagt tacttatcag ctcgatagaa actactacaa tgagtagcat acataccaac
     2161 ataattccaa acgctaccac acgtgcaatc gtttttccaa caggtgcgat ggtgttcacc
     2221 gttttctcaa tgaaatctcc ggtgttacga cgcgtttttt ccatgttctc ggaagaaaat
     2281 gttgatttta cttcttcgcc gaatgcattc atgcgttgtg aaaattgttt cgctccatcg
     2341 cgaacggtgc gctcgatatt gtcgatatca attttctcgc cgcgcatctc caatttttct
     2401 gctgtggttt ttgcttcggg aatgatgatc cacagaatga tatataacaa caatcctgtt
     2461 cctgcaaaaa agaaggcaat caaaaacgcc aatcgcaacc aaagcgcatc gatgtcaaat
     2521 ttagcggcga gtcccgaaca tactcctcct aatacattat gttccttatc gcggaaaaac
     2581 ctggtagttg atggcccttg gcgatattgt tgttgctgtc cggtggaaga ttgctcagat
     2641 gatgccgatt cgaaatcttc cggtgcaccc atcaatgcaa tcacctcctc cacatattgc
     2701 atggtgatgg cactgcgctc gccaactttt tccagaaata attctgctat gcgattttcg
     2761 atatcgcgga taatttcttc tccttctgca gtattcgcat atttttttcg aatgctttct
     2821 aaatactgat caagggtgcg atatgccgta tcatcaatct gaaatacaac accgcctata
     2881 ttgacaaata ataacttgtt cattgctggg taagtttgat gtttttagtg ttaatagaaa
     2941 cggcatgaac caattcctgc catgttttat ctaattcgga tagaaatttg cggcccgtat
     3001 cagttaactg ataatatttg cgcggtggtc ccgaattact ctccacccat ttgtagctca
     3061 atagttccat cgttttcaac ctgttcagca gtggatatac cgttccctcc gctactaaca
     3121 agttgctttc tttcaggcga ctgatcacat ccgtagggta gatctcccct tcgctaatga
     3181 tagccagtat gcagaactcc agcacacctt ttcgcatctg agcctgggca ttctctatat
     3241 tcataaggca aaggtattgc atcgtatggt attatgcaat acatagtagc atgtttttct
     3301 tagttcttgg tgttataatt tgttaaggtg ctgataatca atgagttaaa atttctattt
     3361 ttttggtaaa tcagtttggc aagggttttg tatattaata tatggtcccc aagtaccctt
     3421 tttgtgccct gtcagccctt cttattttta cggctagctg ctctatgatt ccacctgtgc
     3481 aggaagcccc gcttggcatg gaagaaaatt gcggactagg ggcagaagct tggggactgc
     3541 gaggcagcga acctacgcca tgcacgggaa gtgagcaggt tgtgatgaag gataccgatt
     3601 tttctccccc tattcaaatc actcatgcca ctaaaaaacc ggattcagct acgaaatata
     3661 cacatgccgg agataaacag cagtatgtca cattaacaaa acccgaggtt caagagcagg
     3721 attctgatgt gggggaaaca gaggaacgca ctttgcatcc tgcggccatt gcagggctca
     3781 ttttaaccat tttaggaggg ggaattctat ctccgattgg ctgggtgggc atcattttga
     3841 gcgtggtcgc ccttaaaaaa attaaaacga atcccgatat ctattatgga aaagggcttg
     3901 caataaccag tatgatatat ttcattatat ctatcattgc attaatagcc ctgataatat
     3961 tcgcgattta tttcagcgga atatttttat ccattcccta atcattattt caaccaccaa
     4021 taggtccatt cgcttacatg ttcatcgctg atatgcacga aaatattttg tgcaggatac
     4081 gcatatactt ctccgcgacc ggtcagaaat attttagatg gcttgccata taatgcaatc
     4141 aattgttgta aagaatctcc tttgcgcaaa cctttagatg tttttaccgc cggattttcg
     4201 ctgctcgaaa taaaacgcaa tttgctatat gccggtttaa aatttgcgcg cacgtaagtg
     4261 agtatatact cggaagtggt atcggtaaat aacaccaact cttcattttg tacatcgaag
     4321 cggcctgctc tgtttggcgg caaattatcc atgaaatgaa aaatgctttc tccatttaac
     4381 atttctgttg tatcggtagc atatgccgat aatgtattgt caattttaat caatccgttt
     4441 gaagcgtctc gataaacaca tgcataatta tatgttgcgg tctgactgcc ttttgttgtt
     4501 gcgctgctaa acaattgcgt tgcttcggca tcatcatcct gagcagcagc gattatggcc
     4561 cgcatttcat ccacactcca caataacgca ttgtttgcac ccatgcttcg cgatgcattt
     4621 tgcagtgcat ctgtggctga gccgtaagcg ccgcgcaaat aatacaagat tgcaagattc
     4681 atccaggccg gataaaactg tttatcgagt ttgaccgcat tatttaataa tgtgttggct
     4741 tcatcgatgg tcgtattcat tgcatccagc attacaggat ctgttacacc gtttgcactt
     4801 ctgctgcgtt cacttttata taatatactg cccgtttcca ccatgaacgg atattgcaaa
     4861 ttctgcagac tgtcgggaaa catcggaata acgctcagta acctggcaat tgcggcatta
     4921 ttatacattt cctttgtctg cattttgcgt gcaatgatat catcgaattg caatgcagca
     4981 gttttcgcca atagcatttg ctgatcgccg cccatcattt gtgccagcaa cagacaatta
     5041 tttcctgttt cgaaaacggg aatgagtttt gcaatattgg ctcgcgcttc aatgctgatt
     5101 tgtttgcgct cagttaggct tggatatccg ggcaattgct cgttgagatg aaaccaggta
     5161 taaatactat ccaacacttt atcaccgata tacaaagtgc gatagcctgc ggaaaaagca
     5221 taatacatac catattcatc tgcctgcgtt tcatagagtc gcattacatt gagcgatttt
     5281 cccgactgtt gcatttgttc accccaacca acatcggcat aagcagagcc aaattgttca
     5341 gaccaaaaat gattttgata atagtgtgtg agttcgtgcg acaatacgtg agctaatgca
     5401 ttgctcgaat ccttgccgaa gcttctgcat ttatttacca gctgataacc aatggtaacc
     5461 tttccatttc cggaagttgt tgccaacata ttaggatctg gaacaacttc gaggattggt
     5521 tttggtggtg atgataaatt aatggtgcga atgagtttgt tgaacacatc ctgtgcatat
     5581 ttatattcaa atggcgtttg tgcatacaat aatgcattgc cgattgacaa catcattacc
     5641 aataaacaca aacgccacag aatatttcgc atcattatta tttcgattga tttacatcaa
     5701 gatcggtatg aggtttttca ccaaattttt caggcgtcaa ctgcgtttgc atggttgtcg
     5761 atgaaggtgt tacctcgcgg ataaatgtgc cgtcggaata atattccaaa gtcattgttc
     5821 ctgaacttga aatatataaa ccatatccat tcactttgcc ttttacattt tcgcctatca
     5881 ttttatcgcc gccgctaaat gttacagaga acacacctgc aggtgttcct tgcacaaaat
     5941 aaccggtttg tttagagccg tcggtgaagg tccatgtgcc gtaaccatct tccagtgcat
     6001 ctttaaattc accttcataa actaatccgc tgctatattt tttgctgcct tttccattga
     6061 ggcaatcgcc ggtaacacaa tcggcagtgg cgtaatcgac agcggcaaac aatccttgaa
     6121 ttctatttgc ctgatgacca tcgcttgcgg catagccata tgactgacca ttcccgagca
     6181 aaccgaaatt ccattttccg gcttttactt ttccattggc ataagtataa ataccatagc
     6241 cgtgcatgtt attatcgagc cattcgccca tataactatc gccatttggc caggtcattt
     6301 ttccgaggcc gctgaaagat tcatccttga aagcgccttc atatactctg ccatcggtga
     6361 atgtataaac accatagcca ttgcgcacaa accctgtatt taatactcct tgatagcgat
     6421 cgccgttatt atatgtccag ttgccaaaac cggaaacctg cacatcgttg gcgtacatgc
     6481 aagtaccgta gctgccatcc ggattataca acacgccttc accttcgcgc ttacctgctt
     6541 tccattgacc tgcgtaagca tttccatcag gccatgtata aatgccataa ccgctgcgtt
     6601 caccatttac aaagtggcct tcatagcgct gaccgttgtt ccatttcatt acaccataac
     6661 cattattaca atcgccggaa gcacaacctt ctttggtgag aatttcgagt cgcacaaatc
     6721 cgatgtcgag ccaaaaagct ttataattaa ataatgccca gtcgtcgtat gacacccaga
     6781 tataaccatt ttctccccac agtgaactcc atgaattcat aatttgaaat gcgccgccat
     6841 atttattatc gtcatatccg ataattgtca tggcatgtaa ttgatgcata ccgtgattgt
     6901 cgagtacggc ctgacctgga tttgtacgct cttcgtatgt tggtgtccaa tgatcggtgc
     6961 ccattgcctg cataaatgaa tttgccacga atccgccaaa aacaacagga ttatttgatg
     7021 cgatggcaga tttgattgca tcgatatccg gtttcgcttt tgttgttaaa aatttggtga
     7081 ccgcagcagc tgtgcgttct tgtggaaaat cctgcacatc atataaaata tatgcatcat
     7141 taattttgta ttgcgatgca tcggctgtca tttcaggcgt tacacccgtg ccacagccaa
     7201 ttaccggcat ataaaatcgc ttcgatccgt tgtccatcat gaataccaaa acatcttcca
     7261 gtgaatttcc ggaagagcaa ttggcatcgc catttacctg attgtaaaca aagtaagggc
     7321 agaatgaaag tgccgtgatt aggttgcgat cagtcatacc cattttaatt gcccacgctg
     7381 tagtgtatgc agaataagtt gatgaccagg atgcacaggt gccgtattgt ccctgacttt
     7441 tcacataagg tgtgtatgcc atcagcgacc aggctttggg catttcagat ccgtaaccct
     7501 tagcatcagg tttttttgca cgaaaagtgt tgtgtaacgc gggattttcg accataccca
     7561 tccccatctg ggcgtttgca gcaacagtta gacaaactgc agccacaaag gtta
//
LOCUS       DAOQHY010000148         7585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_5059_length_7585_cov_5.085392, whole genome shotgun sequence.
ACCESSION   DAOQHY010000148 DAOQHY010000000
VERSION     DAOQHY010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7585
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5059_length_7585_cov_5.085392"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2654
                     /locus_tag="PLL28_11350"
     CDS             <1..2654
                     /locus_tag="PLL28_11350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF69963.1"
                     /translation="VDLPADQLIGQLAALAPDLKLPSAESVKGKMFKFVMSQAKGDAI
                     RLVEELRAKGTAVITLGPLEGITMLLMVCMITGFVLLRAVLMTKKINTRSLAGMMIWL
                     VFFSAFGAYIFHTGITKKEEETKRLFALKKSIEKDPITEYLFAGAETEIRHQLEMQET
                     AVEQGSRLRPGVIGQVRQNLQSNYFKKYDTDIFLFENDSLLITTDQQNPASRIGFIDA
                     IENNGEFTAAENLYFINDYTGNYYYLAELTTPIDSNKSLTTYLRMVPRKFSGASIYPE
                     LLIDDDLRTAESFIDFDYAIYNDHSLTEREGNYSYPLMDIFYINKDEESADIMENDYD
                     HYIYKVNDNKKVIVSTPAISWFQPVSLFSYLFFFFLVFLVIVLVLDWGAGLLQGKIRL
                     KEWIDTTLQNKIQSAVLSLIIFAFIMMGVATVFYISNQYNDSHKQRLLEKIEGVQTNI
                     DFLLGENKRKTAGAPVNTQLKKIGNRVTELSEIHAMDINIYKPSGALLASSQPDIFDK
                     GLVSRNMNPLAFFKMSCDKETWYIQTERIGDLSFLSAYVPVLDSEGETMFFLNLPYFA
                     TEQNLKAEISSFMVTLVNVYVLLLIVAGIIAFFVSRSITKPLAAIAGKFREIKLGKSN
                     EPIFWTHDDEIGILVSEYNKMLKELDNSAQLLARSERESAWRDMAKQVAHEIKNPLTP
                     MRLSIQHLQRAIQTNAPNVQELTRKVTVTILEQIDNLTHIASEFSNFAVMPKPVNEQI
                     CLNDVLDSVTALFNETENAAITLEMPDERIMIFADKNQVIRVFNNVIKNAIQAIPDDR
                     EGIIAVSLESNEHRALVKVKDNGRGIGDEEKDKVFVPNFTTKSSGMGIGLAMTKNIVE
                     GAGGYIWFESTVSVGTTFYIEFPLMTV"
     gene            2654..3952
                     /gene="hemL"
                     /locus_tag="PLL28_11355"
     CDS             2654..3952
                     /gene="hemL"
                     /locus_tag="PLL28_11355"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HNF69964.1"
                     /translation="MIARDKSTALFEEAKTLFPGGVSSPVRAFRSVEGAPLFMQRGEG
                     ALVWDEDGNQFIDYCCSWGPLILGHADKRVTQAVQEALVKGTSFGTPTRYENIMGQLI
                     INNHRFIEKLRFVSSGTEAVMSAIRLARGYTGRNKIIKFEGCYHGHSDALLVKAGSGL
                     VTLGETSSKGVPAAFANETIVVELDDEAAISSAIEQYPNDIACIIIEPVPANNGLLLQ
                     RKEYLQFLRNICDKHNIILLFDEVISGFRLGFEGASGLYNIQPDLITFGKIIGGGLPV
                     GAYGGKKEIMGMVSPDGPVYQAGTLSGNPLSISAGIAQLQACLTSGFYEELHRKCRII
                     ADKVNRFTAEAKLPFNILTTGSIFWLNFTEDCVIRKASDIKSTMPALFKPFHRYLLEH
                     GIYIGPSGYEVCFVSAAHTDEQLEQTADIIMQAITHTFQK"
     gene            4045..4956
                     /locus_tag="PLL28_11360"
     CDS             4045..4956
                     /locus_tag="PLL28_11360"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNF69965.1"
                     /translation="MHRNNLDNFNDVVAREKFVFTQERGPFLKFEDTANYKRIYDDFK
                     RTELMLYGEGLVFLFLIGLGFWRIRKTFREEIAMTRQQNNFLLSITHELKSPLASLKL
                     SMQTVQKRQLDAEHIRHLSGMALDDIARLEALVENILLASKIESANFQLNRELLDVSS
                     LVTNILAHIAEKHKSQRQFTSDIEKDAYCYGDRLSFSSVVYNLIENAINYSGADGRIH
                     VQLTGDEEHIYLQISDNGIGVPDAEKTKIFNRFYRIGAEETRTTKGTGLGLFIVKHVV
                     ELHGGKIEVTDNDPKGTNFKVTIPVGA"
     gene            5032..5730
                     /locus_tag="PLL28_11365"
     CDS             5032..5730
                     /locus_tag="PLL28_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF69966.1"
                     /translation="MLNHRILVVEDEDHIRENLKLNLELEGYEVVTAADGAKAVKTFR
                     EQRFDLVILDVMLPEMDGFDVCEQIRLEDRETPILFLTAKDTSLDKIKGLKTGGDDYM
                     TKPFNLEELLLRIINLLKRSSKAPAEDKFNTYNFGGNSVNFVTYEANGINGNVKLTNK
                     ELKLLKLLIDRKNEVVSRTLILQTVWGYDVYPSTRTIDNFILAFRKYFESDPKNPEYF
                     HAIRGVGYKFTDKV"
     gene            5850..>7585
                     /locus_tag="PLL28_11370"
     CDS             5850..>7585
                     /locus_tag="PLL28_11370"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HNF69967.1"
                     /translation="MKRLLFLVVLCFCISTTAIFGQLVINEGSNRNGAVLIDEDGDYE
                     DWIEIYNNGLETDLYGYGISDDATLPLKWTLPNQTISANGFALLFASGKDRKPLLTYN
                     HWEQPISDTATWKYIIPSAATPSTWIQPAFDDAAWLSGNASVGYGDGDDATVVPDLTR
                     SVYLRYHFNVADVTLIEEGLLSMDFDDGFIAYLNGHVIAMYGFAAASPAYNAFSDSDH
                     EAAMYGGGVPEHFNISADSINAWLMTGGNVLAVEVHNVSAGSSDLTARPFLSLAVTAP
                     DIIWTDVLPAWFPIGGGTSFLHTNFTIAPSGEMIYLSDPAGLIIDSLFVGVADADQSN
                     GRITDGAADMQIFTTPTPNASNGGTSYPGYTLGDVTFTLDAGFYSGSQTIGITHPAGT
                     IARYTLNGEIPTSTSPIYLVPITIDTNTVFRVRLFDLLGELLPGRTFTNTYFIDENIT
                     TAVISISTNEDNLYGANGIFDNWWTDWKKLCYVEYFDTLGINQFEQQCGFKVDGGAGG
                     SRSLAQKSMRIEPDNPAFGDGTLNYPLIPRRWDTDEYETFYLRNGSNMSNVLPYKDAF
                     MVRTTEGTYNEH"
BASE COUNT         2265 a         1450 c         1675 g         2195 t
ORIGIN      
        1 acgtggacct gcccgccgac caattgatcg gacagctcgc cgcgctcgcc cccgacctca
       61 agctcccgtc cgccgagagc gtgaagggaa agatgttcaa gttcgtgatg agccaggcga
      121 agggggatgc catccgcctc gtcgaggagc ttcgcgccaa ggggacggcc gtcatcacgc
      181 tcggtccgct cgaaggtatc accatgctcc tgatggtctg catgatcacc gggtttgtgt
      241 tgttaagagc agtgttgatg accaaaaaaa tcaataccag gtcgctggca ggaatgatga
      301 tttggctggt atttttctca gccttcggag cttatatttt tcacaccggt attactaaaa
      361 aagaagagga gacaaagcga ctatttgcac tgaaaaaatc tatagaaaaa gacccgatca
      421 cagagtattt atttgcagga gcagaaacag aaatcagaca tcagttggaa atgcaggaaa
      481 ctgccgtaga gcagggtagt cgcttgcgtc ccggtgtaat tggtcaggtt aggcaaaatc
      541 tgcagtcgaa ctattttaaa aaatatgaca cagatatttt cctgtttgaa aatgattctt
      601 tactcataac caccgatcag caaaatccgg caagccgaat agggttcatc gatgccattg
      661 aaaataatgg tgaatttact gctgccgaga atttgtattt cataaatgac tatacaggaa
      721 actattatta tctggcggaa ttgactacac ctattgattc caataaatcg ctcacaacat
      781 atcttagaat ggtgccgagg aaattcagtg gcgccagtat ttatcctgaa ttgttgatag
      841 atgatgatct gcgtactgca gaaagcttca tagattttga ttatgctata tacaacgacc
      901 actcacttac tgaacgcgaa gggaattatt cctatccatt gatggatata ttttacatca
      961 acaaagatga agagtcggcc gatatcatgg aaaatgacta cgaccactac atctacaaag
     1021 tgaatgacaa taaaaaagtg attgtttcaa cacccgctat cagttggttt cagcctgtgt
     1081 ctttattcag ttatttgttt ttcttctttc tggtgttttt agttattgtg cttgttctcg
     1141 attggggcgc gggattatta caaggaaaaa ttagactaaa ggagtggata gatactacct
     1201 tacaaaataa gatacaatca gcagtgctta gccttatcat tttcgcattt attatgatgg
     1261 gtgtggcaac agtattttat atcagcaatc aatataatga ttcgcataag cagagactgc
     1321 ttgagaagat agaaggtgtg caaacgaata ttgactttct tcttggtgaa aataagcgca
     1381 aaactgcagg tgcaccagtg aatacccaac tgaagaaaat aggcaacagg gttacagaat
     1441 tatccgagat acatgccatg gatatcaata tctacaagcc gagtggcgct ttgctggcat
     1501 cgtctcaacc cgatattttc gacaaaggat tggtgtcgcg aaacatgaat ccattggctt
     1561 tttttaaaat gagttgcgat aaggaaacat ggtatattca aacggagcgc atcggcgacc
     1621 tgtcattttt atctgcctat gtgccggtat tggatagcga aggagaaacc atgttctttc
     1681 tcaacctgcc ttatttcgcc actgaacaga atttgaaagc agagatatcc tcattcatgg
     1741 ttaccttggt aaacgtttat gtgttgttgc ttatcgttgc aggtattata gctttttttg
     1801 tgagcagaag tattaccaag ccgcttgcag caattgccgg aaaattccgc gagataaaac
     1861 ttggtaaatc gaatgagcct attttttgga cgcatgatga tgagattgga atattagttt
     1921 ctgaatataa taaaatgtta aaggagctgg ataattcggc tcaattactc gcgcggtcag
     1981 aacgtgaaag tgcttggcgc gacatggcaa aacaggtggc acatgaaatt aagaatccgc
     2041 ttacgcccat gcgtttgagt atccaacatc ttcaacgcgc cattcaaaca aatgcaccaa
     2101 atgtgcaaga gcttacgcga aaggttacgg ttactatttt agagcaaata gataacctta
     2161 cacatatcgc atctgagttt tccaacttcg ctgtgatgcc gaaacctgtt aatgagcaga
     2221 tatgtttgaa cgatgtttta gatagtgtaa ccgcattatt taatgaaaca gaaaatgccg
     2281 ccattacact ggaaatgccg gatgagcgaa ttatgatttt cgccgacaag aatcaggtta
     2341 tccgtgtatt taataatgta attaaaaatg ccatccaggc aatacccgat gaccgcgaag
     2401 gcattatagc cgtgagcctg gaaagcaatg aacatagggc tttggttaaa gtgaaggaca
     2461 atggccgcgg aatcggcgat gaggaaaaag ataaagtgtt tgtccctaat tttacaacca
     2521 aaagttcagg catgggaatt gggcttgcca tgacgaaaaa tattgttgaa ggtgccggtg
     2581 gttatatatg gtttgagtct accgtttctg ttggcacgac attttatatt gaatttccgt
     2641 taatgaccgt gtgatgatag caagagataa atcaacagcc ttatttgaag aagcaaaaac
     2701 attatttccc ggcggcgtaa gttctccggt aagagcattt cgcagcgttg agggggcacc
     2761 tctttttatg caaaggggcg aaggagccct tgtatgggat gaagatggta atcaattcat
     2821 tgattattgt tgcagttggg gtccgcttat tttaggacat gccgataaac gtgttacaca
     2881 agcagttcag gaagcgcttg taaagggtac ttcctttggc acgcccacgc gctatgaaaa
     2941 tatcatgggg cagctcatta tcaataatca taggtttata gaaaaactgc gatttgtttc
     3001 ttcaggtact gaagctgtga tgagtgccat tcgcctcgca cgcggctata ccggtaggaa
     3061 taaaatcatc aaattcgaag gctgctatca tgggcatagt gatgctttat tagttaaagc
     3121 agggtccgga ctcgtgacac tcggggaaac aagttcaaaa ggagtacctg cagcttttgc
     3181 aaatgaaact attgttgtgg aattggatga tgaagcagcg atttcatcgg cgattgaaca
     3241 atatccaaat gatatagcat gtattattat agaacctgtt ccggcaaata atggattgtt
     3301 gttgcaacgc aaagaatatt tgcagttcct aagaaatata tgtgataaac acaatattat
     3361 tttattgttt gatgaagtga tcagtggatt ccgacttggt tttgaaggtg cctcgggctt
     3421 gtacaacata caacccgacc ttatcacgtt cggtaaaatt attggtggcg gattgcctgt
     3481 aggtgcctac ggcgggaaaa aagaaattat gggaatggta tcgcctgatg gacctgttta
     3541 tcaggccgga accttaagtg gtaatccatt gtctatttca gcggggatag cgcaattgca
     3601 agcatgtttg acatccggat tttacgagga attacatcgc aaatgcagga tcattgccga
     3661 taaagtaaat agatttacag ccgaggcgaa attgcccttt aatatactta caaccggttc
     3721 tattttctgg ttgaatttta cggaagattg cgtaatacgt aaagcatctg atattaaaag
     3781 cacaatgcct gcattattta aaccatttca tcgttatttg ctggagcatg gtatctatat
     3841 cggtccatcg ggttacgagg tttgttttgt ttctgctgca cataccgacg agcagctaga
     3901 acaaacggca gatatcatta tgcaggcaat cacacataca ttccaaaaat aacaatgccc
     3961 ggacaacgcc gatcgagata ctggttattt ttccatattg tatttatata cattacggta
     4021 tcttttttgt ggtggtcgta cctgttgcat cgcaataatc tggataattt caatgatgta
     4081 gtcgcgcgtg agaaattcgt atttacccaa gagaggggtc cattcctgaa atttgaggat
     4141 acggcaaatt ataagcgcat ttatgatgat tttaaacgca ccgaattaat gttatatggc
     4201 gaaggattgg tgtttttatt tctcataggt ctcggtttct ggcgcattcg caaaacattc
     4261 agagaagaga ttgccatgac gcgccaacaa aacaactttt tgctgtcgat tacgcatgaa
     4321 ttgaaatcac cgctggcatc tttgaaactg agtatgcaaa cggtgcaaaa acgtcaactc
     4381 gatgccgaac atatcagaca ccttagcggg atggctttgg atgacattgc gcgacttgaa
     4441 gcattggttg aaaatattct actcgcttcc aaaatagaaa gtgcaaattt tcaacttaat
     4501 agggaattgc ttgatgtatc ttcattggta acaaatatcc tcgctcatat tgcagaaaaa
     4561 cataagtcgc aacgtcagtt cacatcagat atagaaaaag acgcatattg ttatggcgac
     4621 cgactttcat tttcttcggt tgtatataat cttatcgaaa atgccattaa ttacagtggt
     4681 gccgatgggc gtattcacgt gcaattaacc ggcgatgaag agcatattta tttgcagata
     4741 tccgataatg gtattggtgt tcccgatgct gaaaaaacaa aaatatttaa cagattttac
     4801 cgcataggag ctgaggaaac tcgtaccacc aaaggtacag gccttggatt atttattgta
     4861 aaacatgtag ttgaacttca tggcggtaag attgaggtca ctgataatga tccaaaaggc
     4921 accaatttta aggtgacaat tcctgtcggc gcttaaaagg acaataagat gacaattgtg
     4981 ttaaaaccct acatttacat ctgcgtataa ttccatcaaa ccctacctac tatgttgaac
     5041 caccgtattt tggttgttga ggatgaagat cacatccgcg aaaaccttaa actaaacctg
     5101 gaacttgaag gctatgaagt agtaaccgct gcagatggcg ctaaagctgt aaaaaccttt
     5161 cgtgaacaac gtttcgacct tgttattttg gatgtcatgt tgccggagat ggatgggttt
     5221 gatgtttgtg agcagataag gcttgaagat cgcgaaacac ctattttgtt cctgacagcc
     5281 aaagacacat cgcttgataa gatcaaaggc ctgaaaaccg gaggcgatga ttatatgacc
     5341 aagcctttca accttgagga gctgttactc cgcatcatta atttgttgaa acgcagcagc
     5401 aaggcgcctg ccgaggataa attcaatacc tataatttcg gtggcaatag tgtgaatttc
     5461 gttacctatg aagcaaatgg tattaatggc aatgtgaaac tcaccaataa ggaattgaaa
     5521 ctcctgaaac tactcatcga caggaaaaat gaagtcgtat caagaaccct tattctccaa
     5581 acagtttggg gttatgatgt ttatccgtcg acccgtacca tcgacaactt catattagct
     5641 tttcgtaaat atttcgaatc cgaccctaaa aatcctgaat acttccatgc catcagaggg
     5701 gtaggatata agttcaccga taaagtataa tcagtagttt tcctgctgat accggcacaa
     5761 actaaggtta tctgaggttc tgatgcaggc tagtcgactc tttgtgtgta taaatttggt
     5821 atttttacct agtaatccgg tacacacaca tgaaaagact cctctttttg gtagtgctat
     5881 gtttttgcat ttctactact gcaattttcg gtcaactggt catcaacgaa ggatcaaatc
     5941 gcaatggtgc ggttttaatc gatgaagatg gcgattatga agactggatc gaaatataca
     6001 acaatggtct tgagacagat ctttatggct atggtatctc tgatgatgcc acattgccat
     6061 taaaatggac attgcccaat caaacaattt ctgctaacgg attcgcttta ttatttgcat
     6121 caggaaaaga tcgcaagcca ttactcacct ataaccattg ggaacaaccc ataagtgata
     6181 ctgctacttg gaaatatatt attcctagtg ccgcaacacc ctcaacatgg atacaacctg
     6241 cgttcgacga tgctgcttgg ttgtcgggaa acgcctctgt aggatatggc gatggagatg
     6301 atgcaactgt ggtgcctgac ctcacacgaa gtgtttattt gcgatatcat tttaatgttg
     6361 cggatgtaac attaattgaa gaagggttat tgagcatgga tttcgacgat gggtttattg
     6421 cttatctcaa tggacatgta attgccatgt acggttttgc tgctgcttct cccgcatata
     6481 atgcattcag cgacagcgat catgaagctg ccatgtatgg aggtggagtg cctgaacatt
     6541 ttaacatcag tgcagattct attaatgcat ggttgatgac aggtggcaat gtccttgcag
     6601 ttgaagtgca taatgtatct gcaggttcaa gcgatttaac tgcacgtccg tttctcagtc
     6661 ttgctgttac tgcgcctgat attatttgga cagatgtttt accggcatgg tttccaatag
     6721 gtggcggaac ttcatttttg catacaaatt ttacgatcgc cccatccggt gaaatgattt
     6781 atttgagtga tcctgccgga ttaattatag atagcttatt tgttggtgtt gccgacgcgg
     6841 atcagtcaaa cggcagaatt actgatggag cagcggatat gcaaattttc actacaccaa
     6901 ctccaaatgc atcaaatggt ggaacgtcat accccggtta tactttaggc gatgtgacat
     6961 ttactttaga tgcaggattt tattccggat cacaaacaat aggcattaca catcctgccg
     7021 gaaccattgc cagatataca ttgaatggcg aaataccaac ttccacaagc ccgatttatc
     7081 tggtgccaat caccattgat acaaatactg tttttagagt aagattattt gatctgctag
     7141 gtgaattgct gcccggtaga acattcacca atacctattt tattgatgag aatattacca
     7201 ctgccgtaat ttctatttct accaatgaag ataatttgta cggtgcaaat ggcatatttg
     7261 ataattggtg gacagattgg aaaaaactgt gctacgtaga atattttgat accctgggaa
     7321 ttaatcaatt tgaacaacaa tgtggattta aggtagatgg cggagcaggt ggcagccgta
     7381 gtcttgcaca aaaaagtatg cgcatagaac ctgataatcc tgcctttggc gatgggactt
     7441 taaattaccc gctcatacct cgcagatggg ataccgatga atatgaaaca ttttatttgc
     7501 gcaacggcag taatatgagc aatgtattgc catataaaga tgcctttatg gtaagaacaa
     7561 ccgaaggtac ttacaatgag cacat
//
LOCUS       DAOQHY010000149         7538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_5106_length_7538_cov_4.432313, whole genome shotgun sequence.
ACCESSION   DAOQHY010000149 DAOQHY010000000
VERSION     DAOQHY010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7538
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5106_length_7538_cov_4.432313"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            527..1102
                     /locus_tag="PLL28_11375"
     CDS             527..1102
                     /locus_tag="PLL28_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69968.1"
                     /translation="MLRSLTIIAMIAFFAAALISADEPKHLIYHDGNYKLKWKDFQGE
                     VEDGFEGFTAVSYVGFRFGYKGTAHQDSLQVVVEAFFNPENSWHRNDTSKAILQHEQI
                     HFDITELYSRKLKQRITQSTLTYKNYQQAMRDMEKEVRAAMNTADSIYDATTDNGLIK
                     AEQATWNTNIAADLENLKVFEPDTFYVPVHL"
     gene            1257..1991
                     /locus_tag="PLL28_11380"
     CDS             1257..1991
                     /locus_tag="PLL28_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69969.1"
                     /translation="MQIRKIAAALLLLATLPVAGLKAQSLNDVLKAHFDAVGQNKVAK
                     LENMTTIGKLNQSGLDIPFKQIVARPGKLRVEGTFQGLTFIQVFNGTEGWAVNPFAGS
                     TEPQPIPADQLDDLKIQADMDGMLWDYAKKGSTVTLEGTEDVEGTPCFKIRIVTADSS
                     VFDEYIDTDSYMLIKSHSKTQMMGVDVETDTYFSNYMQKDGIAMPGRIENRYNGVTGE
                     VITIESVEFDLEYQDALFDKPAAKQE"
     gene            2318..5392
                     /locus_tag="PLL28_11385"
     CDS             2318..5392
                     /locus_tag="PLL28_11385"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69970.1"
                     /translation="MKYLASILFLLLASTELESQSIISDATIGALEGRKIGPARTSGR
                     IAAIDAVDKDPNIVYVGAAGGGVWKSINQGTTFKPIFEDFSQCIGAIAIDQQHPDTVW
                     VGTGEPWVRNSVGVGTGIYKTLNGGEKFQLMGLENSERIAGILIDPKNSNTVYVAVMG
                     ALWGDSEDRGVYKTTDGGATWTRILYVNPSTGCASITMHPTEPNTLLAAMWDHRRTAY
                     DFRSGGPGSGLYQSKDGGATWTKYQDGLPNETLGRICVSYSPVAPYTAYALVESEHSA
                     LYKYSDNDHKWLKMSEQSNMGERPFYFSLLVPDPIDPNRIYKPGFNLTVSNDGGKIFQ
                     SAAVTGGGYHSDLHALYVSPKDNRLMYLGTDGGVYISRDKGNTWSHCENLPVAQFYHV
                     SFDNADPYNVYGGLQDNGSWKGPSSSPGGIQNSDWETIGYGDGFNAYADKTDNNTVYW
                     QYQGGRVYRTNLTTGESKFIKPFPDAGTADLRYNWNAPVVFGKKSNDLYIGSQYLFRS
                     KDKGDTWQRISPDLTTNDPTRQQQEKSGGVTIDNSSAENNTTIFSICESPLDDQVIWV
                     GTDDGNVQVTVDGGKNWTLLNANIPGLPAKAFISYIDADNFEKGGAYITVDAHRNGDK
                     KAYVYYTNDYGKTWQRIDKEPIMGFCHVIKQDLVNKDLLYLGTEFGLYVSIDHGANWV
                     RFKSGVPMVGVYDMAIHPRDRDLVLATHGLGVIIIDDLTILRSITPALLDQEVAFIPT
                     RPYYFPKGGVVQDFPGDDEFIGRSRNGAAVVAYYLKKRHVFGDMFIEIYDSKGNFVRK
                     QPAGSRKGVNIVQIQTNLRPPVVPSSPAILAEAAFGPELEVGDYTIKLIKGTDTFSTK
                     LTLRYNEASNHSVADRQQRRNTMMDAYNLLERLAWYDNTILEIGAQTRETADSIKNRN
                     FEAQLLKTADRMDALHESISATQEGEGGLAAQVRLREKIAEVYSAVAGYDGKPTNVQT
                     DALAKYTADVDAIEKELNALLHGDIETYNKALIKQGLPPIVTSTREVFMSKQ"
     gene            complement(5394..5999)
                     /locus_tag="PLL28_11390"
     CDS             complement(5394..5999)
                     /locus_tag="PLL28_11390"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNF69971.1"
                     /translation="MKRNLLLAVICASILTLPVNAQETSLMQPSGITISAGYGFPSLA
                     KKVFDFAEGDNVNSSFFGPAYVKAEIPLSDHVGFGLNFAYSHGNATYVTQDGEIDSIF
                     YNTQFDYTSYSVLARLNFHFGQEGGMFDPYAGFGMGYRNANYAYTGGDPDAEPYDING
                     LIHLGAELTIGTRIYLTDNIGIYGEVGFAKSPFQAGLVVKF"
     gene            complement(6061..6519)
                     /locus_tag="PLL28_11395"
     CDS             complement(6061..6519)
                     /locus_tag="PLL28_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69972.1"
                     /translation="MKAFLVALLGFGILQSMTGQTAVTDPKKNEALVTIGTMSALSLY
                     NTYVAIGAVADAYGRDVYTAQNVGDLMKEQLTILPTAKAQVEKLKYSGYLEDQTDIDY
                     CQRIMDCLDLLILEAGGLSEYALHGDLDAQLNYANAREDAWELITKILQL"
     gene            complement(6542..>7538)
                     /locus_tag="PLL28_11400"
     CDS             complement(6542..>7538)
                     /locus_tag="PLL28_11400"
                     /inference="COORDINATES: protein motif:HMM:NF014822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HNF69973.1"
                     /translation="MLYLDYSRKEGEWIANDYGGNENLEAIQFLKELNETLYSEVPSV
                     ITIAEESTSWPKVSKPTYDGGLGFGMKWMMGWMHDTLQYFKEEPVYRKYHQDKLTFSM
                     WYAFSENFILSLSHDEVVHMKGSLINKMPGDEWLKFANLRLMFAYMFTHSFPKLMFMG
                     GEFAQKKEWNHDQSLDWHLVEEPSHRGIQLLVKDLNHLYTGEKSMYENDFKQAGFEWL
                     DINRRDQCVLVWKRAGKQANDHLIVVGNFDQQTHIDINIPVLQRNHYVEILNTDAEKY
                     YGSGLINPGLIKPKTVVVKNDDETEDKQYELTLTIAPLTLIVLKPVAKLRRSKKS"
BASE COUNT         2064 a         1832 c         1743 g         1899 t
ORIGIN      
        1 caaacctata ctcctcgcca cttccagatg tccagttcct tccttgcgtt atcatcccaa
       61 acggatcata atctaaaacg atgaaattta gagtttgcat gggtttcggc tgatctaaat
      121 taatgtattt tgaacagaat aatgcctttt tatacatatt tcagcttcat tttctcagtt
      181 ataaacttgt cgatgaaagt tagtgatatg ttaaggatct atttcaccta atataattct
      241 tttaattgac ggttatgcga taactatcgg gatggccgat ttgggttggc tctgcgcctg
      301 ccttgccggc aggcaggcct ctgcgtgaaa aaccctccgc tccacaatca cgactcacga
      361 ttcacccttg ttcaatcgcg gagtttgcgg agttagcgga gtcagtggat cagtcaccat
      421 tcacgactca cgagtcacga ttcacgaaac actcacccac tgacccactc acccactgac
      481 ccactcacca ttcacagcct ttcttacctt tgcacaaacc tccaccatgc tccgatcgct
      541 taccataatt gccatgattg cattcttcgc cgctgcactg atctcagccg atgagcccaa
      601 gcatctcatt taccacgatg gcaactacaa attgaaatgg aaagattttc agggcgaggt
      661 ggaagatgga tttgaaggat ttaccgctgt ctcctatgtg ggattccgat tcggatataa
      721 gggaaccgca catcaagaca gcctgcaggt ggtggtagaa gcctttttca accccgaaaa
      781 cagctggcat cgcaacgata cttctaaggc gattcttcag cacgaacaga tccacttcga
      841 catcaccgag ctgtattccc gcaagctcaa gcagcgcatt acacaaagca cgctgacata
      901 taagaattac caacaggcta tgcgcgacat ggaaaaggaa gtgcgtgctg ctatgaacac
      961 tgccgatagt atctatgacg ccactaccga caatgggctt attaaggcag aacaggcaac
     1021 ctggaatacc aacatagccg cagatctgga gaacttaaag gtcttcgaac cggatacttt
     1081 ttatgtgccc gtgcatttgt aactgtagag tctgattggg cattaagcgc tttgatttag
     1141 gcgaaaaccg gaaattcacc ggctattttc agtctataat taaccttgtg ttaacacctc
     1201 gggaagtacg gcgcacatag acaggtcgta attttaaccc acaatttcaa ctcatcatgc
     1261 aaatcagaaa gatcgccgca gctttgttgt tgctggcaac cctccccgtg gccggactaa
     1321 aagcccaatc actcaacgat gtgctaaaag cgcattttga cgccgtagga caaaacaaag
     1381 tggcaaaact ggaaaatatg accaccatag gtaagctcaa tcagagcggt ttggatattc
     1441 catttaagca gattgttgcg cgcccgggca agttaagggt tgaaggtact tttcagggcc
     1501 tcaccttcat acaggttttc aatggtaccg aaggatgggc tgtgaatccc tttgccggat
     1561 ctactgaacc gcagcctata cccgccgatc aactcgacga tctgaagatc caggctgata
     1621 tggacggtat gttgtgggat tatgctaaaa aaggcagcac cgttactttg gagggcaccg
     1681 aagatgtgga aggcactcct tgtttcaaaa tcaggattgt gactgccgat tcttcggttt
     1741 ttgatgaata catcgatacc gattcttaca tgctcatcaa atcgcatagc aaaacccaaa
     1801 tgatgggggt tgacgtagaa acagatacct atttcagtaa ctatatgcaa aaagatggca
     1861 tcgccatgcc gggaagaatt gaaaaccgct acaacggtgt gaccggcgaa gtgatcacca
     1921 tcgagtctgt tgagttcgac ttagaatacc aagacgcttt gttcgataaa ccggctgcca
     1981 aacaggaata ataatttata aattatatat atgcattttt tataaatgac tgtaatagaa
     2041 tgatatatag tagtttatat catgtaatgt agtattgttt cacgcagaga cgcagaggcg
     2101 cagaggaatg caaactcacc actcacccta gttcaatcgc ggagtttgcg gagttagcgg
     2161 agtcagtgga gtcagtggat cagtaaccac tcaccaacaa ttggcaaagc aagttcatca
     2221 aaacattacc ctccgaacca cactcacgat tcacgagtca cgattcacga aacactcacc
     2281 cacccgccta tcggcgaggc aagctcaccc actaacaatg aaatacttag caagcatact
     2341 attcctcctc ctcgcaagca cggagttaga gagccagtcg atcatttctg atgctactat
     2401 tggggctctt gagggaagaa agattggtcc tgcgaggaca agtggtcgca tagcggcgat
     2461 tgatgcggtg gataaggatc cgaacattgt atatgtgggc gctgccggcg gcggtgtgtg
     2521 gaagtcgatc aaccagggga cgacattcaa gcccattttt gaagatttta gtcagtgcat
     2581 tggcgccatc gccatagacc agcagcatcc cgataccgtt tgggtgggga ccggcgagcc
     2641 atgggtgaga aactctgtgg gtgtgggaac gggaatttat aagaccttga atggcgggga
     2701 gaagttccag ctcatgggac ttgaaaacag cgaacgcatc gccggcatcc tcatcgaccc
     2761 gaaaaattcg aataccgttt acgtggcggt aatgggtgcc ttatggggtg atagtgagga
     2821 tcgcggggtg tataagacca ccgatggcgg agcaacctgg accaggatat tgtatgtaaa
     2881 cccatctacc ggttgcgcct ctattaccat gcatcctacc gaacccaata ccctgcttgc
     2941 agctatgtgg gaccatcgcc gaaccgctta cgacttcagg tcgggaggcc cggggagcgg
     3001 actttaccaa agcaaggatg gtggggcaac gtggacaaaa tatcaggacg gattacccaa
     3061 tgaaaccctt ggcaggatct gtgtaagcta ctcaccggtg gcaccatata ccgcctatgc
     3121 cttggtggag tctgaacatt cagcattata caagtattca gataacgatc acaagtggct
     3181 gaaaatgagc gaacaaagca acatgggtga acgtccgttt tactttagtc tgctcgtacc
     3241 cgatccaatt gaccctaacc gcatctacaa gcccggcttc aacctgaccg taagcaacga
     3301 tggcggcaag atcttccaaa gcgcagcggt aaccggtggt ggataccaca gcgatttgca
     3361 tgccttgtat gtgagtccga aagataaccg cttaatgtat ttgggaaccg atggcggtgt
     3421 ttacatctcc cgcgacaaag ggaatacctg gagtcattgc gagaacctgc cggttgcgca
     3481 attttatcac gtgagcttcg acaatgcgga tccttacaat gtgtatggcg gtctgcaaga
     3541 caatggcagt tggaaaggtc cttcatcctc ccccggaggt atacagaact cagattggga
     3601 gacaataggt tatggcgatg ggttcaatgc ctatgccgac aagacagaca acaataccgt
     3661 ttactggcaa taccaaggtg gtcgagttta ccgcaccaac ctgaccaccg gcgaaagcaa
     3721 attcatcaag ccctttcctg atgccggcac cgccgacctg cggtataact ggaatgcacc
     3781 ggttgtgttt ggcaagaaat cgaacgacct ctatatcggc agccagtatt tatttcgttc
     3841 caaagacaag ggagatacct ggcagcgtat cagtccggac ctaactacca atgatcctac
     3901 gcgtcagcaa caggaaaaaa gcggtggtgt taccatcgac aactcctctg ccgagaacaa
     3961 taccaccatt tttagtatct gcgaatcgcc actcgatgac caggtgatat gggtaggaac
     4021 agacgacggc aatgtgcaag taactgttga tggcggaaag aactggaccc tgctgaatgc
     4081 aaatattccc ggccttcctg ctaaggcttt catctcgtat atcgacgccg ataacttcga
     4141 aaaaggcggc gcatacatca ctgtagacgc acatcgcaat ggcgacaaga aggcatatgt
     4201 ttactacact aatgattacg gtaaaacatg gcaacgtatt gataaagaac caattatggg
     4261 attttgtcat gtgatcaagc aggaccttgt gaataaggat ctgctatacc ttggaacaga
     4321 attcggttta tatgtttcaa ttgatcatgg ggctaactgg gtgcgtttca aatcgggtgt
     4381 gccgatggtg ggtgtgtatg acatggccat tcatcctcgc gatcgcgacc tggtattagc
     4441 cactcatgga cttggcgtga tcatcattga cgaccttacg atattacgca gcatcacccc
     4501 ggccttactg gatcaggaag tggctttcat ccccacccga ccatactact tcccaaaagg
     4561 aggggtagtt caggactttc cgggagatga tgaattcatc gggcgcagca ggaatggcgc
     4621 tgcggttgtg gcctattacc tcaaaaaacg ccatgttttc ggcgatatgt tcatcgagat
     4681 ctacgacagc aaaggcaact tcgtgcgcaa acagcctgcc gggagtcgca agggtgtgaa
     4741 tatcgtgcag atacagacaa acctgaggcc accggttgtg ccatcctccc ctgccatact
     4801 tgcagaggct gccttcggtc cggagttgga ggtcggcgac tataccatca aattgattaa
     4861 aggcacagac acctttagca ccaagcttac cttgcgatat aatgaggctt caaaccattc
     4921 ggtagccgat cgtcagcaac gcaggaatac catgatggat gcctacaacc tgctcgaacg
     4981 ccttgcctgg tatgacaata cgattctgga aatcggcgcc caaacgcggg aaactgctga
     5041 ttccatcaaa aacaggaact tcgaagctca actcctgaaa acagctgatc gcatggatgc
     5101 cctgcatgaa agcatcagcg ccacgcagga aggcgaaggc ggattggcgg cgcaggtgcg
     5161 tttgcgtgag aaaatcgctg aagtgtattc ggcagtagcc ggttatgacg gcaagccgac
     5221 gaatgtgcaa actgatgcat tggcaaaata taccgctgat gtcgatgcca ttgaaaaaga
     5281 actcaatgcc ctgctacacg gcgatatcga aacgtataac aaggcattga tcaaacaggg
     5341 cttaccccct atcgttacct ctacccgaga ggtattcatg agcaagcaat aggttagaat
     5401 ttcactacca accctgcctg gaagggcgat ttggcgaagc ctacttcccc ataaatacca
     5461 atattatcgg taaggtaaat gcgtgtacct atggtaagct cggcacccag atgtattaaa
     5521 ccattgatat cataaggttc agcatcgggg tcgccacctg tataggcgta attggcattg
     5581 cggtaaccca ttccaaaacc tgcatacgga tcaaacatgc ctccctcctg tccgaaatgg
     5641 aagttcaatc gcgccagcac actataagag gtatagtcga attgggtatt gtaaaagata
     5701 ctgtcaatct cgccatcctg agttacatag gtggcattcc catggctgta agcgaagttg
     5761 agtccaaacc ctacatgatc actcaggggg atctcagctt tcacatatgc aggtccgaaa
     5821 aaggaactat tcacgttatc gccctcggca aagtcgaata cttttttcgc caggcttgga
     5881 aaaccgtatc cggcggaaat agtaataccg gatggttgca ttagagaagt ctcctgtgcg
     5941 ttaacaggta gagtcagtat acttgcgcag ataacggcta acaacaaatt ccttttcata
     6001 agtatggcgg attacagcca cacttaccta tccaaacgct gtgccatttt tttcaaaccc
     6061 ttacagctga aggattttgg tgattagctc ccaggcatct tcccgcgcat tcgcgtaatt
     6121 tagctgagca tctaaatcgc catgcaaagc atattcgctc aatccaccgg cttctaaaat
     6181 caacaaatca aggcagtcca tgatcctttg gcaataatcg atatccgttt gatcctccag
     6241 gtaaccggag tatttcagtt tttcgacctg ggcttttgct gttggcagaa tcgtaagttg
     6301 ttctttcatc aggtccccga cattctgagc ggtgtaaaca tcccttccat aggcatctgc
     6361 tacagcacct atggcaacgt aagtattata taacgacagt gccgacatcg tcccgatagt
     6421 caccagggct tcatttttct tcggatctgt tacagcagtc tgaccggtca ttgattgcaa
     6481 aataccaaag cctaaaaggg ctacaaggaa tgctttcata ttgatatgat ttacagtgaa
     6541 attaagattt tttgcttctt ctcagcttgg caacaggttt cagcacgatg agggtaagcg
     6601 gtgcaatggt gagggtaagt tcatattgtt tatcctcagt ttcatcatca ttcttaacca
     6661 ctactgtttt cggttttatc aaacccgggt ttatcaatcc gctgccatag tacttttcag
     6721 catcggtatt caatatttcc acataatggt tgcgttgcag caccggtatg ttgatgtcga
     6781 tatgcgtttg ttgatcaaaa ttaccgacaa cgatcaggtg gtcgtttgct tgttttcctg
     6841 cccttttcca aaccaggaca cactggtcgc ggcgattgat atccagccat tcgaaaccgg
     6901 cctgtttgaa gtcgttttca tacatgcttt tttctccggt ataaaggtga ttcaaatcct
     6961 ttacaagcag ctgaatcccc ctgtgagaag gctcctccac caaatgccag tcgaggcttt
     7021 gatcgtgatt ccactctttc ttttgagcaa attcaccacc catgaacatg agtttcggga
     7081 aggaatgtgt gaacatatag gcaaacatca atcgcaggtt ggcgaacttc aaccattcat
     7141 ctcccggcat tttattgatg agcgagccct tcatatgcac tacttcatcg tgcgagaggc
     7201 tgaggatgaa gttctcgctg aaggcatacc acatactgaa agtaagctta tcctgatgat
     7261 acttccgata tacaggctct tccttaaaat attgcagcgt atcgtgcatc cagcccatca
     7321 tccacttcat accaaagccc agaccaccgt cgtacgttgg ttttgatacc ttgggccagg
     7381 aggtggattc ttctgcgatg gtgattactg acggcacctc gctatagagt gtttcattga
     7441 gttctttaag aaattgaatg gcttccagat tctcatttcc cccataatca ttggcaatcc
     7501 actccccttc tttgcgcgaa taatcgaggt acagcata
//
LOCUS       DAOQHY010000150         7397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_5240_length_7397_cov_6.759602, whole genome shotgun sequence.
ACCESSION   DAOQHY010000150 DAOQHY010000000
VERSION     DAOQHY010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5240_length_7397_cov_6.759602"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2551
                     /gene="mfd"
                     /locus_tag="PLL28_11405"
     CDS             <1..2551
                     /gene="mfd"
                     /locus_tag="PLL28_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNF69974.1"
                     /translation="NMLHVDHPFRQHPPQELLLSADDLLSEVPGFSWIEFGLNSSFPD
                     ARTIVFDTVPQPLFNRNFETLIAQLKELRSKRYDIFIFMSNARQAERFHEIFTDLNAK
                     VDYTPVIPIIKEDGKPSTTIAQGFIDHDMHVVCFTDHQLFERHHSYRVKSGYSKNDAV
                     TLKTLMELRPGDYVTHIDHGVGVYSGLEKIEVNGQIQEAVRLVYRDNDLLYVNINSLH
                     KISKYVGKEGTEPKLNKIGSDAWEQLKRKTKTRVKDIAKDLIQLYAKRKAAKGFAYSQ
                     DGYLQNELEASFIYEDTPDQNRATVDVKRDMEKPYPMDRLVCGDVGFGKTEIAIRAAF
                     KAVCDGKQVAVLVPTTILATQHYKTFSERLKDFPVRVDYINRFKSDKEKKQTLELLEA
                     GKIDILVGTHAIIGKKIKFKDLGLLIVDEEQKFGVTVKEKLRAFRANIDTLTLTATPI
                     PRTLQFSLMGARDLSIINTPPPNRQPIQTEIKSFDADFIRDAIYHEIYRGGQVYFIHN
                     RVRDIEETAALIKKICPDLHVGVAHGQMEGDVLEDHMMQFITRNYDVLVCTNIVESGL
                     DIPNANTIIINNAHHFGLSDLHQLRGRVGRSNKKAYCYLISPPVFALPDDSRKRLKTI
                     EQFADLGSGFQIAMRDLDIRGAGNLLGGEQSGFIAEIGYDVYHKILDEAIRELKQTQF
                     KEIFADQVHEKQEYVRDVQIDTDLEMLIPDEYISSISERMTIYTRLDNISNEEQVTAF
                     KNELADRFGKLPKQVEELFNGIRLRWLAKDLGMERIIFKSRKLRCYFVENSASVFYDS
                     PLFQNIMQAIAQKRSPGTIKQTDKHLILVFEQVQSMQHAKSLLEALTKAVV"
     gene            2598..3134
                     /locus_tag="PLL28_11410"
     CDS             2598..3134
                     /locus_tag="PLL28_11410"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF69975.1"
                     /translation="MILETPRLILREVTPDDVDGFYKLDSDAEVHRYLGNQPVTSLQQ
                     CKDVIAFVMKQYVDNGIGRWAVVEKFSGDFIGWAGLKLVKEETNGHVNYYDYGYRLIR
                     QYWGKGYASECSPFILQYGFTHLPADIIYGAAHVNNIASNRILRKNGFQFKNTFFYES
                     DLCNWYALEKSLFEGNLP"
     gene            3229..5292
                     /locus_tag="PLL28_11415"
     CDS             3229..5292
                     /locus_tag="PLL28_11415"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNF69976.1"
                     /translation="MSTQTVRLTAEFKKQTTRVVIVILLFVLVYLILIALAVGLTAAS
                     IAGGIFIILASPAFITLVLGVGMASLGSLILIFLLKFLFTSNKYDRSHLIEVTRAEEP
                     ALFDMIDDIVEQVKTAKPKKVYWSADVNAAVFYDSSFWSMFLPIRKNLIIGIGLLNTL
                     TAEELKAVLSHEFGHFSQRTMKVGSYVYNVNKVIFNMLYDNDSFGSMASRWGSVSGYI
                     AIFVMLAFHIINGIQYLLRKMYELVNKSYSSLSREMEFHADEIAASITGYEPLKTALM
                     RFSISSASYNEVLNYYNEKAAQNIKSENIYADQTLVLKFLAEINKIRVENDLPNVTEE
                     EQNRFNKSRLVIKDQWASHPTTKDRVTRLENTGFHASGLQHQPAKTLLRNAGNTYKQV
                     TANLFSNVTYKGNTSFESPETLITAYREKFESNAFPAIFNSYYDDKNIEVFNPGEVTP
                     STETILMEELYSDKAVESVYAEIGLASDINVLKDIGNGFTQVKSFDFDGIRYSRKKAL
                     TLVDNLKPELDKLTAENKANDIRIYATFISLEQKLGLAPKLQTMYQTLFDYAADLERK
                     LQIYSHLASKLEFVSVQTPHEEIDRRLALVRPLEDKLKTEINKILSDTFYQSEITEDM
                     MKNMQSFTSRHIEYFLLGNYVEENLSLLYKAMNNYRYLVLRAYFILKRNILLYQAELH
                     QKTVA"
     gene            5397..6089
                     /locus_tag="PLL28_11420"
     CDS             5397..6089
                     /locus_tag="PLL28_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69977.1"
                     /translation="MKSMITGAAALIFFLTTAFYPGDNCESYFANKVGTSMEQTSYDA
                     KGKMTSKSVSTVKAYDAIDGGFASTLNVRVMDAKDKEVSNSDVIMKCQQDKFYMDLSN
                     AFPKEMAGMEGAEVEMENTFVEFPAHPVAGQSLPDQTSSMTIKLNGTPFMTMTINTTN
                     RKVEGFESMTTTAGTFECMKYSADTEVKSMFTSKSHSVMWMSKNVGLVKMESYDDKGK
                     LQATQMLTKFSE"
     gene            complement(6125..7369)
                     /locus_tag="PLL28_11425"
     CDS             complement(6125..7369)
                     /locus_tag="PLL28_11425"
                     /inference="COORDINATES: protein motif:HMM:NF005455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HNF69978.1"
                     /translation="MEQHKFRPESLMMSFGYKPELSEGAVKCPIFQTSTFVFRNAEAG
                     KRFFEVAYGLSSPEPGEEQGLIYSRLNNPDLEILENRLTLWDEAEACAVFSSGMSAIS
                     TVLLEFLQPGQYVLFSAPVYGGTDHFIKHVLPKWGIGHAAFDANSSEEEIHAIIQEKG
                     ADALGLIYVETPANPTNALIDMEMCKRIADVYSNDKKVYLAVDNTYMGPLWSHPLKHG
                     ADIVMYSATKYIGGHSDVIAGAVLGGNEVMTRIKTMRTFMGNMMSPNTGWLLMRSLET
                     LKPRMELQAQNAMKVVEFLNEHPKVEKVYYLGLIPEGTPEYHIYRNQYSSPGAMLSFD
                     IIGGEKEAFTFLNSLKLIKLAVSLGSTESLAEHPATMTHADVSPEDKQKLGISEKLIR
                     LSVGVEYYEDLIFDIERALSHV"
BASE COUNT         2273 a         1413 c         1582 g         2129 t
ORIGIN      
        1 aaacatgctg catgttgatc atccattccg acaacacccg ccgcaggagt tattgctgag
       61 tgcagatgat ttgctgagcg aagtacctgg attcagttgg atagaattcg gactcaacag
      121 tagttttccg gatgcccgta ccattgtttt tgacaccgtt ccgcaacctt tgtttaacag
      181 aaatttcgaa acacttatcg cgcaactaaa agaactgcgc agtaagcgat acgatatttt
      241 tattttcatg agtaatgcac gtcaggcgga gcgttttcat gaaatattca ccgatctgaa
      301 tgctaaagtt gattatactc cggtaattcc aattatcaag gaggatggca aaccttcaac
      361 cactattgca cagggattta ttgatcacga tatgcatgtg gtctgtttta ccgaccatca
      421 gctatttgag cgacatcata gctatcgcgt aaaatccggt tattcaaaaa atgatgcagt
      481 taccctgaaa acattgatgg agttgcgtcc gggagattat gtaacacata tcgatcatgg
      541 cgtgggcgtg tattcgggtt tggagaaaat tgaggttaac ggtcagatac aggaggcggt
      601 gcgtctggtg tatcgcgaca atgatctgtt atacgtaaac atcaatagtt tacataaaat
      661 ctctaaatat gtaggcaagg aaggaacaga gccgaaatta aataaaattg gaagcgacgc
      721 atgggaacaa ctcaagcgta aaacaaaaac acgtgtaaaa gatattgcta aagacctgat
      781 tcaattatat gcgaaacgaa aagcggctaa aggatttgca tatagtcagg atggatacct
      841 tcaaaatgaa cttgaggcaa gctttatata tgaagataca cctgatcaaa accgcgctac
      901 tgttgatgta aaacgcgata tggaaaaacc ctatcctatg gatagactgg tttgtggcga
      961 tgtgggcttc gggaaaacag aaattgccat ccgcgcagca tttaaggccg tatgtgatgg
     1021 taaacaagtt gctgttttgg tgcctaccac tattttggct acacaacatt ataaaacatt
     1081 ttccgagcga ctgaaagatt ttcccgtaag agttgattac atcaatcgat ttaaatctga
     1141 caaagagaaa aagcaaacac tagaattatt ggaagccggt aaaattgaca ttttagtcgg
     1201 cacacatgcc atcataggaa agaaaattaa attcaaagat ctgggtttgc tcattgtaga
     1261 tgaagaacag aaattcggtg taacggtaaa ggaaaaattg cgcgccttta gagctaatat
     1321 tgatacactg acattgacgg caacaccaat tcctcgaacc ttgcagtttt cgttgatggg
     1381 ggcacgcgat ctgagtatta ttaacacgcc acctcccaat cgtcagccca ttcaaactga
     1441 aattaaatcg tttgatgctg attttatcag agatgccatt tatcatgaaa tttatcgcgg
     1501 cggacaagtt tactttattc ataatcgggt aagagatatt gaagaaactg cagctctcat
     1561 caaaaaaata tgccccgatt tgcatgtcgg tgttgcacat ggtcaaatgg aaggagatgt
     1621 gttggaagat catatgatgc aattcatcac caggaattac gatgtattgg tgtgcacaaa
     1681 tattgtggaa agcggacttg acattcccaa tgctaatact atcattatca ataatgcgca
     1741 tcatttcggg ttgagcgact tgcatcaatt gcgcggcagg gtagggagga gcaataaaaa
     1801 agcatattgc tacctcatat caccaccggt ttttgctctt ccggatgatt ccaggaaacg
     1861 gctcaaaaca attgagcagt ttgctgatct gggaagcggt ttccaaatcg ccatgcgcga
     1921 tctcgacatt cgtggagcgg ggaatttgct gggcggtgaa caaagtggat ttattgctga
     1981 aattggttat gatgtatatc ataaaatact tgatgaagcc atacgtgaat taaagcaaac
     2041 acaattcaaa gaaatctttg ccgatcaggt gcacgaaaaa caagagtacg tgcgcgatgt
     2101 gcaaattgac acagatcttg aaatgttgat acccgatgaa tacatctcaa gcatctcgga
     2161 acgcatgacc atttatacaa gactggataa tatctcaaat gaagaacaag taacagcttt
     2221 taaaaacgag ctggcagatc gcttcggaaa attgccaaaa caggtggaag agttgtttaa
     2281 cggaattcgc cttcgttggt tggcaaaaga cctgggtatg gaacggatta tttttaaaag
     2341 cagaaaactc agatgctatt ttgtcgagaa ttcggcttct gtattttatg acagtccgct
     2401 gttccaaaat attatgcagg ctattgctca gaagagaagt ccgggcacaa taaaacaaac
     2461 cgacaagcat ctgattttag tattcgagca ggtgcaaagc atgcaacatg ctaaatcatt
     2521 gctcgaagca ttaacaaaag cagttgtctg acaatactgc tgttatagcc atttctataa
     2581 attcggtaaa ttagtatatg attctagaaa caccgcgact tattcttaga gaagtcactc
     2641 cggatgacgt cgacggtttt tataaacttg attccgatgc tgaagtgcac cgctacttgg
     2701 gtaatcagcc ggtaacaagc ctgcaacaat gcaaagacgt catcgccttt gtgatgaagc
     2761 agtatgtaga caatggcatt ggtcgctggg ctgtagttga aaagtttagt ggtgatttta
     2821 tcggctgggc cggattgaaa ttggtgaagg aagaaaccaa tggtcacgtg aattattatg
     2881 actatggata tcgcctcata cgccaatatt ggggtaaagg ctacgcttca gaatgcagtc
     2941 cgtttatttt gcaatatggg tttactcact tgccagccga cattatttat ggagcagccc
     3001 atgtaaacaa tattgcatcc aatcgcattt tacggaagaa tggttttcaa tttaaaaata
     3061 cctttttcta tgaaagcgat ttgtgtaatt ggtatgcact tgagaagtca ttgttcgaag
     3121 ggaatcttcc ttgatggtat ttgcttgatt aagccagaag attacatatt gtaatacttg
     3181 ttgttagagg tagtttgttt tcctatattt gtcgaaagca ttttcctcat gagcactcaa
     3241 actgtgcggc ttaccgccga attcaaaaag caaactacca gagtagtaat tgtaatttta
     3301 ttatttgtat tagtttatct gatacttata gcattggctg tcggactaac ggctgcctcg
     3361 atagcgggcg gaatatttat cattcttgcc tcccctgcat ttattaccct tgttttggga
     3421 gttggtatgg ccagtttagg ttccttgatt ttaatatttt tattgaaatt tttatttact
     3481 tcaaataagt atgacagatc acatttgata gaagtcactc gtgctgaaga gcctgcttta
     3541 ttcgacatga tcgatgatat cgttgagcag gtaaaaaccg ctaaacccaa aaaggtatac
     3601 tggtcggctg atgtaaatgc tgccgtgttt tacgattcca gtttttggag tatgttcctg
     3661 cctatacgaa agaatcttat tattggcatc ggactgttga atacacttac cgctgaagag
     3721 ttgaaagcgg tgttgtcgca tgaattcgga catttctcgc aacgaaccat gaaggtggga
     3781 agctatgtgt acaatgtgaa taaagtaatt tttaatatgt tgtacgacaa cgattcattt
     3841 ggaagcatgg catccagatg gggaagcgtg agtggttata tcgctatttt tgttatgctg
     3901 gcttttcata ttataaatgg gatacaatac ctgctcagga aaatgtatga gctggtaaat
     3961 aaaagctata gcagcctttc cagggaaatg gaatttcatg ccgatgagat tgcagcgagt
     4021 ataacgggtt acgagccatt aaaaactgcg ctgatgcgat tttcaatatc ctctgcatct
     4081 tataatgaag tgttgaatta ttataatgaa aaggctgctc aaaatataaa aagcgaaaac
     4141 atctacgccg atcaaacact tgtcttgaaa tttttagcgg agataaataa aattcgggtt
     4201 gaaaatgatc tgccaaatgt tacagaagag gagcaaaaca gatttaacaa atcgcgattg
     4261 gtaattaaag accaatgggc tagtcacccg acaacgaaag acagagtcac ccgcctggaa
     4321 aataccggtt ttcatgcaag cggattacag catcaacccg caaaaacctt gcttcgtaat
     4381 gcaggaaaca cctataaaca ggttacagcc aatttatttt ccaatgtaac ttacaaagga
     4441 aacacctcat ttgaatcacc ggaaacactc ataacagcat atagagaaaa gtttgagtct
     4501 aatgctttcc cggcaatttt taattcatac tacgacgaca aaaacattga agtattcaat
     4561 cccggagagg taacaccttc cacagaaacg atcttgatgg aagaattgta ttctgacaaa
     4621 gctgttgaaa gtgtatatgc ggaaattggt ctggcgagtg atatcaatgt actaaaagat
     4681 attggcaatg gatttactca ggtgaagtca tttgattttg acggtatccg ttattcgcga
     4741 aaaaaggcgc tcacgttagt tgataatttg aagccggagc tcgataaact tacggctgaa
     4801 aacaaagcaa atgatattcg tatctacgcc acatttatct ctctagaaca aaaattagga
     4861 ttagcaccta aattgcagac catgtatcaa acactgtttg attatgccgc tgatttagaa
     4921 aggaaattgc aaatttattc gcatctggct tctaaactcg aattcgtaag tgttcaaaca
     4981 ccgcatgaag aaattgatcg cagattggcg cttgtaagac cgcttgaaga taaattgaaa
     5041 accgaaatca ataaaatatt atctgatact ttttatcagt cggaaatcac cgaagatatg
     5101 atgaaaaata tgcagtcatt tacttccagg catattgagt attttttgct tggtaattat
     5161 gtcgaagaaa atttgtctct actatataag gcaatgaata attatcgata ccttgttttg
     5221 cgagcttatt ttattttaaa aagaaatatt ctgctttatc aggcagaatt gcatcagaaa
     5281 accgttgcct aagttgttga gtttgagcag tgtgtgtatt aatcactatc ctaagattga
     5341 ttttcctcac tccaataaca ttgcaggata ttgtaacttt gtataaaatc cgttttatga
     5401 aaagcatgat tacaggtgct gcggcgctta ttttcttcct cactactgcc ttttatcccg
     5461 gcgataactg tgaatcttat ttcgctaata aagtcggcac atctatggaa cagaccagtt
     5521 atgatgcgaa ggggaaaatg acttctaaat cagttagcac tgtgaaggcc tatgacgcta
     5581 tagatggtgg ctttgcctct actttgaatg ttcgtgttat ggatgccaaa gacaaagaag
     5641 tatcaaacag cgatgtaatc atgaaatgcc agcaggataa attttatatg gacctcagca
     5701 atgcatttcc taaagaaatg gccggaatgg aaggtgctga agtggaaatg gaaaacacat
     5761 ttgttgaatt tcctgcgcat ccggtagctg gtcagtcact tcctgatcaa acaagcagca
     5821 tgaccattaa attaaatggc actccattta tgacaatgac catcaacacg acgaatcgca
     5881 aggtagaagg atttgaatct atgacaacaa ctgcaggaac attcgagtgt atgaaatatt
     5941 ccgcagacac tgaagtaaaa tctatgttta cctccaaatc acattcggtg atgtggatgt
     6001 cgaaaaacgt tggtctcgtg aaaatggaga gctacgacga caaaggaaaa cttcaggcaa
     6061 cacaaatgct gacgaagttc tccgagtgat attatgtcaa ataatttgat cctttgacta
     6121 gtgcttatac atgactcaaa gcgcgttcaa tatcgaagat aaggtcttca taatattcta
     6181 cgccgacact taagcgaatt aatttttctg atatacctaa tttctgtttg tcctcaggcg
     6241 atacatctgc gtgcgtcatc gtagcaggat gttcagcaag cgattctgtt gaacctagtg
     6301 aaacggcaag tttgataagt ttcagactat tcaaaaaagt aaacgcttct ttttcacccc
     6361 caataatatc aaaacttaac atggcgccgg gtgatgaata ttgattgcga taaatgtgat
     6421 attcaggtgt tccttctgga attaaaccta aataatacac cttctcaacc ttcggatgtt
     6481 catttagaaa ttcaacaact ttcattgcat tttgcgcttg caattccatg cgcggtttta
     6541 atgtttctaa actgcgcatc agcaaccatc cggtattcgg actcatcata tttcccataa
     6601 atgtgcgcat ggttttaatg cgcgtcatta cttcatttcc acccaaaaca gctccagcaa
     6661 ttacatcgct gtgacctccg atatattttg tagccgaata catcacgatg tcagcgccgt
     6721 gcttcaaagg gtggctccac aatggcccca tataagtatt atcaactgcc aggtaaacct
     6781 ttttatcgtt gctatataca tcggcgatgc gcttgcacat ttccatatcg ataagcgcat
     6841 tggtagggtt ggcaggcgtt tctacgtaga tcaggccaag ggcatcagcg cctttttcct
     6901 gaatgatggc atgtatctcc tcctcagagc tgtttgcatc aaaggcagcg tgtccgatgc
     6961 cccattttgg caatacatgt ttaatgaagt gatcagttcc accatacacc ggtgcgctga
     7021 aaagcacata ttgtccgggt tgcaggaatt cgaggagcac ggtagagatg gcgctcatgc
     7081 cgcttgaaaa cacagcacaa gcttcggctt catcccaaag cgtaagcctg ttttcgagga
     7141 tctcgaggtc ggggttgttg agtcgcgagt agatgagacc ttgttcctcg ccgggttccg
     7201 gactgctgag gccatatgcc acttcgaaga agcgtttgcc ggcttcagca tttcggaata
     7261 cgaaggtgct ggtttgaaat atcgggcatt tcacggcccc ctcactcagc tcgggcttgt
     7321 aaccaaagct catcatcaga ctctcggggc gaaatttatg ttgttccatt ttagatgtgg
     7381 ttgtggcgta aaactac
//
LOCUS       DAOQHY010000151         7271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_5372_length_7271_cov_5.785338, whole genome shotgun sequence.
ACCESSION   DAOQHY010000151 DAOQHY010000000
VERSION     DAOQHY010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7271
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5372_length_7271_cov_5.785338"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(500..1780)
                     /locus_tag="PLL28_11430"
     CDS             complement(500..1780)
                     /locus_tag="PLL28_11430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF69979.1"
                     /translation="MKKKILQILPAALLITGVMQTGHAQIVSGVSFLKGKWIELAVSD
                     CGTYASGGAGMIVAAPATYHENTIDGSLSFCNDVTADGWDIGVPDQCGDFIMPGSPEE
                     GFAIQIGTGPVYGNLQPYCYRFGAFVAAAPDFVGTNLTNVNGGSIRKTLWQGTNTGIG
                     LAVSQTTYWLNSKQSYITIVDLCNGGDDMFDVYYARNADPDNDQITSGTFNTNNNAKK
                     QYATAGYSQVTATSTVGSPCYMAYVSNDSRAKVSRGNFSMGSPSDIWNGLSGYIQTPG
                     AVNADAAIQISFKIDTLLHNDCGCFTYSTMLTSAGLTDQLALTGTACATLGTLARYGE
                     DMIDGYLNDPTNFLKNEMVVYPSPSNGNFTVNIFEMEHANITVVNAVGQVVYQVNDVS
                     KIAGITLDNPVPGMYFVNAEYGEGKKITRSLVIE"
     gene            complement(1786..2172)
                     /locus_tag="PLL28_11435"
     CDS             complement(1786..2172)
                     /locus_tag="PLL28_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69980.1"
                     /translation="MKKLLMPVLLAAGLVFATAGYSQDAVKFDQHFQVQLTDAMLQSG
                     SVVMDISDLQFTDEAAAQKYFNAIRNNLVEFTLDYSAHTVTMHLYPERLGKHTWTLSD
                     WNAYLANMAERNAETYQSFQISSPKQ"
     gene            complement(2456..3796)
                     /locus_tag="PLL28_11440"
     CDS             complement(2456..3796)
                     /locus_tag="PLL28_11440"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HNF69981.1"
                     /translation="MKKNILYTLCAIILIGGLNSCEDILVLEPAQSLSNETALDNDLG
                     VKQTLVGAYDRMAQSSLLGGDVIMNADLYSGAGEFEWVGTYVDYREIFTHDILVNNGS
                     VSSLWDDAYEVINTCNNVINAIDVVEAADQDRVRGEALTLRAWCYFELVRAFGYQYQP
                     GVENTQLGVPLILTPTLNFGDNTNVTRNTVEECYGQIIGDLEQAATLLPEKNDVYVNT
                     YTASALLARVYLQKEDYENARDAADRVIASNDYELMSNVADVFNQDDATDEDVFSVEI
                     STTDGNNSLNNYYATNDFGGRGDIEIQDAHMALYDPLDARATLFFIDGGIRFTSKFNN
                     EFGNISAIRIAEMYLIRAECNQRLGTSVGATPEDDYNAIHTRAGLPAAASVSLDDILY
                     ERHLELCFEGQAIHDIKRLHGMVGTLNYNDPKLIYPIPQTEIDRNALLVQNDGY"
     gene            complement(3826..6891)
                     /locus_tag="PLL28_11445"
     CDS             complement(3826..6891)
                     /locus_tag="PLL28_11445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF69982.1"
                     /translation="MQKFLFLALFSLAGMQLFGQGRTITGRITDADNGVYLAGVTVLL
                     EGTMNGTVSDAEGKYSITVPEEGGNLIFSFIGYENQKITLGTKNVLYVSMAMSQEILD
                     EVVVVGYGTEIKTKVTGSISEVGNDEIALTPVSTVEQTLQGRAAGVFIESNNGKVGSD
                     VRIRIRGNSSINASNQPLVVVDGVPINTVGVNDYFNVVLNPMNDIDFNDIESVEILKD
                     ASAAAIYGSRGANGVILITTKRGKVGKPTVTLDYQYGFSKPTRLREFLNAQEYIDYYT
                     QAAINAGEYDFTNGLSGYTSVEEAIDDYIADAEKKFNKLQGYTDWQNDYANTDWQSLA
                     FQEATSTMFDFSVSGGSEKMQYFTSVGYNYQDGIMIGNHGDRFNITNNMDAQLTEKLK
                     LGTSLTISRSINNDIPDDNSFSTPLQMVAQSPLTPPQDTTGEYYNTPTTLYYNPLIEV
                     DNVFFESTTFRTIGNLSAEYQLRPVLALRAELGGDITNHWQQRFYGSKSEVGLSVGGY
                     ATNFNSTVQYFDTKMLLLYSQKFGKSTLGATTGVEYNQYDDQYNSVEGQGFPSDKLQT
                     LASAAEIVYGTGVLNAYRFLSYFTRITYDYDTKYLFNVTVRADGSSRFGENKRYGVFP
                     SGSFGWVVSNEDFLKGNKTLSFLKVRMSYGMTGNAGIGNYDYKGLYAVGSYAGTSALY
                     PSTIANPDLTWEKTTQADLGIDFGFINDRINGEFDVYLKQTTDLLLDVPVPSTTGFTV
                     QTQNVGSLENKGVEFTINSDNFVRTFTWSTSVNFSANRNKITELANGQTIIDYGGSDF
                     MNVVMVGQPLGVFYGAEYAGVDSENGDGLWYVNDGTDATTNDFNDANFVVVGDPNPDF
                     IAGLTNNFGYKNLSLDVTLQGVYGNDVHMSGDYWMSSNASQWDNQTVDQLNAWQNPGD
                     VTDVPENRLLFENGTAARSSRYLDDASYLRVKTVTLSYDMPKRVTGKLNMSAMRLYAS
                     AYNLFTVTNYRGWDPEVSSDSFVDNIYYGIDFYSAPQPRTVVFGISLGL"
     gene            7021..>7271
                     /locus_tag="PLL28_11450"
     CDS             7021..>7271
                     /locus_tag="PLL28_11450"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69983.1"
                     /translation="MKKCLLPALLFLVASAGQLNAQQAIPPKAERIQHTIHGSFGDRE
                     DPYYWISDRDNPKVIDYLKAENAYTEAVTAPYKKIQEQL"
BASE COUNT         2116 a         1607 c         1418 g         2130 t
ORIGIN      
        1 aaatattgca aagcaatata attgcattcc ctattaacgc gccagcgtta aaagcccata
       61 acacttgcta acttgcaaac ttgctaattt gctaaccctc tttcagtatc tagaatcaag
      121 tatctagtat ctagactttt gcgccttagc ctccttgctg tctttgcgcg aaaccaaccc
      181 gcctcacctc cggcccctct ccatctccta tcgtcgatag agaggggagt tgattgcgct
      241 tcggaattaa aatattgcaa agcaatataa ttgcattccc tattaacgcg ccagcgttaa
      301 aagcactcca cacttgctaa cttgcaaact tgctaatttg ctaattccca aattagctaa
      361 ttaccctcac ttgctaaccc tcaaacttgc tcaccaaaaa taatctccat gcatgcacca
      421 tcccatttgc taattttcaa ataaaaaaaa acaggaccca aatcggatcc tgtcttatga
      481 tgatgtaaaa tcgcttacgt tattcaatca caagcgagcg agtgattttt ttgccttcac
      541 cgtattctgc atttacaaaa tacattcccg gaacaggatt atcaagtgtg atgcccgcaa
      601 ttttgcttac atcattaact tgataaacaa cctgaccaac agcatttact acagtgatat
      661 ttgcatgttc catttcgaaa atattcacag tgaaattacc atttgacggg cttggataaa
      721 ccaccatttc atttttaagg aaattagttg gatcattcaa ataaccatct atcatgtctt
      781 caccgtaacg agcaagtgta ccaagagttg cacaagcagt tcctgtcaat gcaagctgat
      841 ctgtaagacc tgctgatgtc aacattgttg aataagtgaa gcatccgcaa tcattatgca
      901 acagtgtatc aattttgaaa gagatttgaa tggcagcatc tgcgttaacg gcacccggag
      961 tttggatata gccgctaaga ccattccaga tatcagaagg tgaacccata gagaagttac
     1021 cgcgagaaac ttttgcgcga gagtcgttac ttacataggc catataacaa ggagaaccaa
     1081 ctgtactggt tgcagttacc tgagaataac cggcagtagc atattgtttt ttcgcgttgt
     1141 tattggtgtt gaatgtaccg ctagtaatct gatcgttatc aggatcggca ttacgagcat
     1201 aatacacatc aaacatatca tcgccaccat tacaaaggtc aacgatagtg atataagatt
     1261 gtttgctatt caaccaatat gttgtctgag aaacagcaag tccgatacct gtatttgttc
     1321 cttgccacaa cgtttttctg atagaaccac catttacgtt agtaaggttt gttccaacaa
     1381 aatcaggtgc tgcggccacg aatgcaccga agcgataaca atatggctgc aagtttccgt
     1441 atacagggcc tgtaccaatc tgaatggcaa aaccctcttc aggagaaccc ggcatgatga
     1501 aatcgccgca ctgatcagga acaccaatgt cccaaccatc ggctgtaacg tcgttgcaaa
     1561 agctcaatga accatcaatt gtattttcat gataagtggc aggagctgca actatcatac
     1621 ctgcaccacc ggatgcataa gtaccgcaat cagaaactgc cagttctatc catttccctt
     1681 taaggaaaga aacaccgctc acaatttgag catgtccggt ttgcataaca cctgtgatca
     1741 ataatgccgc aggtagtatt tgtagaattt tctttttcat gcgttttatt gtttaggtga
     1801 tgaaatttga aatgactgat aggtttctgc attgcgttca gccatattcg ccaggtaagc
     1861 attccaatcg cttaatgtcc aagtgtgctt gcccagacgt tcaggataca aatgcatagt
     1921 aaccgtgtgc gcactgtagt caagtgtaaa ctcaacaagg ttgtttctaa tagcattgaa
     1981 gtacttctga gcggcagctt cgtctgtgaa ctgcaggtcg gaaatatcca taactacaga
     2041 tcctgattgc aacatagcgt cggtcaattg aacctggaag tgctgatcga acttgacagc
     2101 atcctggcta taaccggctg ttgcgaatac taaccctgcc gcgaggagca cgggcatcaa
     2161 cagttttttc atcggtgaag gttttatttg tgaaaaaaat accgaccctt tgtgagttgg
     2221 tttataaatt tacaaataat gccttagtgg ttctaattat cgataaaatg tgacatttta
     2281 aatcatgtca cattttatca caaaaactca ctgctgaggt ccgggaggca tagttttgga
     2341 aatattctat tcatttgtca tgtcacaaat ggtccatttt tgacaaattt atgttaatcg
     2401 acaaaaaaag gccggaatat cgctatcccg gccttgtgtt gtttctttgt cttgctcaat
     2461 atccatcatt ttgaaccaac aaggcattgc ggtcaatttc tgtttgaggg ataggatata
     2521 tcaatttagg atcgttgtag ttcaaggtgc ccaccatacc atggaggcgt ttaatatcat
     2581 gtatggcctg cccttcaaag cagagttcca agtggcgctc gtataagata tcgtcgagcg
     2641 aaacagaggc tgctgccggc aatcctgcgc gtgtgtgaat agcgttatag tcatcttcag
     2701 gtgtagcacc tacgcttgta cccaaacgct gattacattc agcgcggatc agatacattt
     2761 cagcaattct gatagctgaa atatttccga attcattatt gaattttgaa gtaaagcgaa
     2821 tgccaccatc aataaaaaac aaagtagcac gtgcatctag cggatcatat aacgccatat
     2881 gtgcatcctg aatttcgata tctccgcgtc caccgaaatc attggtggca taataattat
     2941 tcaatgaatt attgccatca gtggtcgaaa tctccacgct gaatacatct tcatctgttg
     3001 catcatcctg attgaataca tctgcaacat ttgacatcaa ttcataatca tttgaagcta
     3061 tcactctatc tgcagcatca cgcgcattct catagtcctc tttctgaaga tatacgcggg
     3121 ccaataaagc agatgcagta taggtattta catatacatc atttttctcc ggtagtaatg
     3181 ttgcagcttg ctcaaggtca ccgatgattt gtccgtaaca ttcttccaca gtatttctgg
     3241 taacatttgt gttatcgcca aaattgagtg taggcgtaag aatcagtggc acacctaatt
     3301 gcgtattttc cacacccggc tgatattgat atccgaacgc ccgaactaat tcaaaataac
     3361 accaggcgcg aagggtgagt gcttcaccac gcacgcgatc ctgatctgcc gcttcaacta
     3421 catcaatagc gttgatcacg ttgttgcagg tattaatcac ttcataagca tcgtcccaaa
     3481 gtgagcttac agatccatta tttaccagga tatcatgtgt aaatatttcg cgataatcga
     3541 cataggttcc tacccattca aattcgccgg caccggaata caaatctgca ttcatgatta
     3601 catcaccacc caacagtgat gattgcgcca tgcgatcata ggcaccaaca agtgtttgtt
     3661 ttacaccgag atcattgtcc agtgcagttt cattgcttaa actttgagca ggctccaata
     3721 caagaatatc ttcacaggag tttaaaccgc ctatgagtat tattgcacaa agtgtatata
     3781 gaatattctt tttcataatt gtcagtttca tttgtttgac atgcattata aaccaagtga
     3841 aataccgaaa acaacagtgc gtggttgcgg agcgctatag aagtcgatac cataatagat
     3901 attatctaca aatgaatcgc tggatacttc cggatcccaa ccgcggtaat ttgttacggt
     3961 gaacaagtta tatgctgaag catacaaacg cattgcactc atatttaatt taccggtaac
     4021 acgcttaggc atgtcgtaac tcagtgttac agttttcaca cgcaaataac tggcatcatc
     4081 gagataacgg ctggagcgtg cagctgtacc attttcaaat aataatctgt tttctggcac
     4141 atccgtaaca tcgccgggat tctgccatgc atttaactga tcaacagttt ggttatccca
     4201 ctggctggca tttgacgaca tccaatagtc gcccgacata tgcacatcgt taccataaac
     4261 gccttgcaag gtaacatcca ggcttaagtt cttataaccg aaattattgg taagacctgc
     4321 aatgaaatca gggtttggat caccaactac aacaaaattc gcatcgttga aatcattagt
     4381 tgtagcatca gtgccatcgt ttacatacca aagaccatct ccattttcag aatcaacacc
     4441 tgcatattca gcaccataga aaacgccgag tggctggcct accattacca cattcatgaa
     4501 atctgatcca ccgtaatcga tgatggtttg accatttgcc agttcagtga ttttatttct
     4561 gttggcagag aaattcacgc tggtgctcca tgtaaatgtg cgtacgaagt tgtcggagtt
     4621 gatggtaaat tcaacgccct tattttcgag gctaccaacg ttttgtgttt gcacagtgaa
     4681 acctgttgtt gaaggaacag gcacatcaag caagaggtcg gtagtttgct tcagatacac
     4741 atcaaattcg ccattaatgc gatcattgat aaatccgaaa tcgataccta agtctgcctg
     4801 agttgttttt tcccaggtaa gatcaggatt ggcaattgtt gatggataca atgctgaagt
     4861 tcctgcgtaa gaaccaacgg catagagtcc tttgtaatca tagtttccga tacctgcatt
     4921 tcctgtcata ccatagctca tgcgtacctt caggaagctg agtgttttat ttcctttgag
     4981 gaaatcttca ttggaaacta cccatccgaa agatcctgat gggaaaacac catagcgttt
     5041 attttctcca aatcgtgaag aaccatcggc acgaacggta acattaaaga gatatttagt
     5101 atcgtaatcg taagtgatac gagtgaaata tgataagaag cgatatgcat tcagaacacc
     5161 agttccgtaa acaatttctg cagcgcttgc aagtgtctgc aatttatctg aagggaatcc
     5221 ttgtccttct acagaattat attgatcgtc atactgattg tattcaacac ctgttgttgc
     5281 acctaaagtg ctctttccaa atttctggct gtataataac aacatcttgg tatcgaaata
     5341 ttgaacggta gagttgaagt tggtagcata accacctaca gacaatccta cttccgattt
     5401 acttccgtaa aaacgttgtt gccagtgatt ggtaatatcg ccacctaatt ctgcgcgcaa
     5461 tgccaatacc ggacgtaatt gatattcagc acttaagttg ccgatggtgc ggaaagtagt
     5521 actttcgaaa aatacattat ctacctctat caacggatta taatataacg tagtaggggt
     5581 attataatat tcacctgtag tatcctgcgg tggagtcaat ggtgattgcg caaccatttg
     5641 taatggcgta gagaatgaat tatcatccgg aatatcgttg ttgatagaac ggctgattgt
     5701 aaggctggtg ccgagtttca gcttttcagt aagctgtgca tccatattat tggtgatatt
     5761 aaaacgatcg ccgtggttgc cgatcataat accatcctga tagttatagc ctacagaagt
     5821 aaaatattgc attttctcag aaccaccgga aactgaaaag tcgaacatgg ttgaagttgc
     5881 ttcctggaat gcgagcgact gccagtcggt attggcataa tcattttgcc agtcggtata
     5941 accttgcagc ttattgaatt ttttttctgc gtcagctata taatcgtcaa tagcctcttc
     6001 aacgctggta taaccactta atccattggt gaaatcatat tcaccggcat tgatggcagc
     6061 ttgtgtataa tagtcgatgt attcttgtgc atttaagaat tcacgcaagc gcgttggttt
     6121 gctgaaacca tattgataat cgagtgtaac ggtcggcttc ccaacttttc ctctttttgt
     6181 ggtgataagt attacaccat ttgcacctcg cgagccatag atggcagcag cagaggcatc
     6241 ttttaatatc tctacacttt cgatgtcgtt aaaatcaatg tcgttcatag ggttcaatac
     6301 cacgttaaaa tagtcgttga cacctaccgt attgattggc acaccatcta caaccactaa
     6361 tggttggttg ctggcattaa tagacgagtt accacggata cggatgcgaa catcacttcc
     6421 tacttttccg ttattggatt cgatgaatac ccctgcagct ctaccttgaa gtgtctgctc
     6481 aaccgtagaa accggtgtta gggcgatttc atcattgcca acctcggaaa tacttcctgt
     6541 gaccttggtt ttgatctctg taccataacc cacaacaacg acctcatcaa ggatctcttg
     6601 tgacatggcc atagacacat ataatacgtt tttagtacct aatgtgatct tttggttctc
     6661 atacccgata aacgagaaga taaggttacc tccctcttca ggcactgtaa tggaatattt
     6721 accctctgca tcagagacgg ttccgttcat ggtcccttcc agtaagaccg taacccctgc
     6781 taggtatacc ccgttgtctg cgtcggtgat acgtccggta atggtacgac cctgtccgaa
     6841 tagctgcatg ccggcaagtg aaaataaagc caggaataga aatttctgca tatctgtcgg
     6901 ttttgtccgt cgctaaagta aggcatttca ctttaacaat agataacatt tatgtaaaaa
     6961 aacaggcgat ttgtgacagg gtaactgtca caatatcaga gggatttgta attttcagcc
     7021 atgaaaaagt gtttactccc ggccttgctg ttcttagtag cgtcagcggg tcaactgaat
     7081 gcccaacagg ccataccacc aaaagccgaa cgcattcagc atactatcca tggcagcttt
     7141 ggcgatcgtg aagatcctta ttactggatc agcgaccgtg acaatccaaa agtaatcgat
     7201 tacctgaagg ctgaaaatgc ctatacagaa gctgttactg ctccatataa aaagattcag
     7261 gagcagcttt t
//
LOCUS       DAOQHY010000152         7263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_5377_length_7263_cov_5.683962, whole genome shotgun sequence.
ACCESSION   DAOQHY010000152 DAOQHY010000000
VERSION     DAOQHY010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7263
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5377_length_7263_cov_5.683962"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2029
                     /gene="dnaE"
                     /locus_tag="PLL28_11455"
     CDS             <1..2029
                     /gene="dnaE"
                     /locus_tag="PLL28_11455"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNF69984.1"
                     /translation="RAIAEDQKDIRSKIIKEALILEGSVRNTGVHAAGLIIAPKDLTD
                     LLPIAVAKDSALYVTQFEGEVIESAGVIKMDFLGLRTLSIIKTALKLIKENHNVIIDI
                     DTIPLDDLKTFELYQRGETNATFQFESPGMQKYLRELKPDKFEDLIAMNALYRPGPLE
                     YIPTYIKRKHGREPIEYDLPQMEEYLKETYGVTVYQEQVMLLSQKLAGFTKGDADTLR
                     KAMGKKQKDVLDKMKSKFLEGADKNNIDKKVCEKIWTDWEAFAQYAFNKSHSTCYAFV
                     AYQTAYLKAHYPAEYMSAVLTHNQGNIDKVSFFMDECRRMGVPVLPPDVNESDLNFAV
                     NKNGQIRFGMSAIKGVGESAVIELINERKKDGAFNSLFDLTRRVSLRSVNKKTFEALV
                     YAGAFDSFGLNRSQYFELAKNETLNLIEQAIRYGNNFNGDTVKSQNTLFGDSEDVEIA
                     EPKIPDFPEWNTIEKLKKEFEVVGMYMSGHPLDDYKIEADAFKTTTITDIEKFTDKGD
                     IVLVGYVASTDTKVGKTGDKYTIFTLEDFSGHLEMGLFKNNYIKFKNYVDQTGNVLYV
                     KGAYQTDRNDPERKRFWVSDIQLLSEVREKKSMKITLSMSLSDLNSNFLEGLSEVLKQ
                     HPGKDSIVLKVIEHTEQFDLLMRNKQGGIRAESAVLNSLGKFGEVNLTVVG"
     gene            2119..2436
                     /gene="trxA"
                     /locus_tag="PLL28_11460"
     CDS             2119..2436
                     /gene="trxA"
                     /locus_tag="PLL28_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNF69985.1"
                     /translation="MAIEFNDANFKQTVLDSGKLSVIDFWAEWCGPCRVVGPVIEELA
                     KDYDGKVNIGKVNVDFNPEISMMYGIRNIPTILFIKNGQVVDKQVGAVPKSVLDAKVR
                     QHM"
     gene            2512..3447
                     /locus_tag="PLL28_11465"
     CDS             2512..3447
                     /locus_tag="PLL28_11465"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNF69986.1"
                     /translation="MDRQLISLQDLHQVENLELIAKQVVEGFILGLHKSPFHGFSVEF
                     AEHRIYNLGEATRHIDWKVFGRTDKLFTKKYEEETNLRCHIVIDASSSMYFPEPDKQA
                     KGLHLNKLIFSAICSSAIIHLLKMQRDAAGLTIFADDVLLHTKAGSSNVHHKMLQTQL
                     ESFIGKSSHDLPTNAGKCLHQIAENIHRRSMVVIFSDMMEDVEKSEELFSALQHLKHN
                     KHEVILFHVTDKRHEINFEYDNRPYIFVDMETGEKIKVRSSEVKEKYTEQMAKYMQQL
                     KLRCGQYGVDLIEADMMEDFRDVLLPYLVRRGKMH"
     assembly_gap    3501..3600
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3944..4285
                     /locus_tag="PLL28_11470"
     CDS             3944..4285
                     /locus_tag="PLL28_11470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNF69987.1"
                     /translation="MERDLVKRIRNFAYNVYRHLKSVRLNFYNRQIMQQLLDSSFSAA
                     ANYRAAQRAKSRKDALNKIKIALEEMDESNFWITSLYDLDESTSSDMQALIKESNELT
                     SILVAIISKMG"
     gene            4649..5410
                     /locus_tag="PLL28_11475"
     CDS             4649..5410
                     /locus_tag="PLL28_11475"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNF69988.1"
                     /translation="MELNYKLLGKGKSVVILHGLFGMLDNWITFGKSLSENYAVFLID
                     QRNHGNSPHSENHSYTLMAEDVYQFCLKHQLHQITLIGHSMGGKTAMKFALMYPELVE
                     TLIVIDIGVKQYSGGHETIFRALRQMDLDAIHSRSEAESFLSEAITEVGVRQFLLKNL
                     TRNTSGKYHWKFNLQALFEHYNETLAPITSTHTFDGKTLFIRGGNSGYIQDKDWDDIT
                     TLFPLAQLHTIANAGHWVHADQPQLLLDLVRNKIA"
     gene            complement(5452..6579)
                     /gene="hppD"
                     /locus_tag="PLL28_11480"
     CDS             complement(5452..6579)
                     /gene="hppD"
                     /locus_tag="PLL28_11480"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HNF69989.1"
                     /translation="MDTITLTTGKNLVEDFLPLNGTDHIEFYVGNAKQSAYYYQSAWG
                     YELVAYAGLETGVKDRASYVLQQGKIRLVLTTSFDPSSEISEHVRKHGDGVKVLALWV
                     DDAAKSYAETTKRGAIGVMEPTRMKDEHGEVVVAAIQTYGDTIHKFVERKNYNGVFMP
                     GYQPKSSNVPVTSVGLKYVDHCVGNVELGRMNDWVKFYEDVMGFKLLITFDDSDISTE
                     YSALMSKVVSNGNGYVKFPINEPAEGKKKSQIEEYIDFYKGAGVQHIAIATDNIIETV
                     TELRARGVEFLYVPETYYEDVLDRVGHVDEDFDELKKLNILIDRDDEGYLLQLFTKPV
                     EDRPTVFYEIIQRHGARSFGKGNFKALFEAIEREQELRGTL"
     gene            complement(6697..7062)
                     /locus_tag="PLL28_11485"
     CDS             complement(6697..7062)
                     /locus_tag="PLL28_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNF69990.1"
                     /translation="MKNQFHPENGIVKRSFQFSLRIIEYCYVLENNRRFNMSYQLFRC
                     GTSVGANVAEAQNAESKSDFIHKMKIASKEASETEYWLRLCLQSPNYPDCQDLLEELE
                     PMIKIIGRIIITSKKSLTT"
BASE COUNT         2253 a         1387 c         1423 g         2100 t
ORIGIN      
        1 acgcgcaatt gccgaagatc aaaaagatat tcgcagtaaa ataattaaag aggcattgat
       61 ccttgagggt agtgtgagga atacaggcgt acatgcagcc ggtttgatta tcgcaccaaa
      121 agatcttaca gatttattgc caattgctgt cgcaaaagac tcggctcttt atgttacaca
      181 atttgaagga gaagtaatcg agagcgcagg tgtaatcaag atggactttt taggtttgcg
      241 tacgctgtcc attattaaaa cagcactaaa gctgattaaa gaaaatcata atgtcattat
      301 cgatatcgat accattccgc tagatgatct caaaactttc gaattatatc aacgcggtga
      361 aacaaacgcc accttccagt ttgaaagtcc gggaatgcaa aaatatttac gcgaattaaa
      421 gcccgataaa ttcgaagacc tgatcgcgat gaacgcctta tatcgccctg gaccgcttga
      481 atatatccct acctatatta agcgtaaaca tggccgcgaa cctattgaat acgaccttcc
      541 tcaaatggaa gaatacctca aggaaaccta cggtgttact gtttatcagg aacaggtgat
      601 gctgttgtcg cagaaattgg caggctttac taaaggagat gctgacacgc tgcggaaagc
      661 aatgggtaag aagcaaaagg atgtattgga taaaatgaaa tccaagttcc ttgagggagc
      721 tgataaaaat aatattgata aaaaagtatg tgaaaaaatc tggacagact gggaagcatt
      781 tgcacaatat gcctttaata agtcgcactc tacatgttat gcatttgtcg cttatcaaac
      841 ggcctatctg aaagcacatt atccggcaga atatatgagt gccgtattaa cacataacca
      901 ggggaatatc gacaaggtaa gcttctttat ggacgagtgt cgccgcatgg gtgttcctgt
      961 tttaccgcct gatgttaatg agagtgatct caattttgct gtgaataaaa acggtcagat
     1021 acgattcggt atgtcagcta tcaaaggagt tggtgagtct gcagtaattg aattgatcaa
     1081 tgaaagaaaa aaggatggtg cgtttaatag cttgtttgat ctgaccagaa gggtaagcct
     1141 gcgaagcgta aataaaaaaa catttgaggc tttagtttat gccggagcat ttgatagttt
     1201 cggattaaat cgctcacagt attttgaact cgcaaaaaat gaaacactga accttattga
     1261 acaagccatt cgatacggaa acaatttcaa tggtgatacc gtaaaatcac aaaatacctt
     1321 atttggcgac tctgaggatg ttgaaatagc agaaccgaag attccggatt tccctgagtg
     1381 gaataccata gagaaattga aaaaggaatt tgaagttgtt ggcatgtaca tgagtggtca
     1441 tccgctcgac gactataaaa ttgaagctga cgctttcaaa acaactacca ttacggatat
     1501 tgagaaattc acggataagg gagacattgt tttggtaggt tatgtggctt caacagatac
     1561 taaagtgggt aaaacaggag ataagtatac catatttaca ctggaagatt tctccggaca
     1621 tctggaaatg ggattattta agaacaacta tatcaagttc aaaaattatg tagatcagac
     1681 cggaaatgta ttgtatgtga aaggagctta tcaaaccgac cgaaacgacc cggaaaggaa
     1741 gcgtttctgg gtaagtgaca ttcagttgct gtctgaggta agggagaaaa aatcgatgaa
     1801 aataaccctt agcatgagcc tgtcggacct gaattcgaac tttttggaag gtctctcgga
     1861 ggttttgaag cagcaccccg ggaaagacag cattgtgctc aaggtgatag agcataccga
     1921 gcagtttgac ctgcttatgc gcaataaaca gggcggtatc agggcggaaa gtgcagtttt
     1981 gaacagtctg gggaaatttg gggaagtcaa tttaacagta gtcggctaat tttgtgtatc
     2041 ccaaacggga acttttacag gtatccgatg ttatgcgtaa tacctgttcg aaaaacagct
     2101 ttaaaccaac ttaagaatat ggctatagaa tttaacgacg caaacttcaa gcaaacagtg
     2161 cttgacagcg gaaaattgag tgtgatcgac ttttgggcag aatggtgtgg cccttgtcgc
     2221 gtagtaggtc cggttatcga agaactggcc aaagactatg atggcaaagt gaacatcgga
     2281 aaagtgaatg tcgattttaa ccctgaaatc tcgatgatgt acggtatccg taacatcccg
     2341 acgatcctgt tcattaaaaa tggtcaggtg gttgacaaac aagttggtgc agtgccaaaa
     2401 agcgtccttg atgctaaggt gagacaacac atgtaattaa tttatactga agaaaagcct
     2461 cctatcgttt gacaggaggc tttttacgtt ttatcttgca ccttaattgc tatggacagg
     2521 cagctaatat ctctacaaga tctccatcag gttgaaaacc tcgaactcat cgccaaacag
     2581 gtggtggaag gatttatcct gggattgcat aaaagtccat ttcatggatt ttcagttgaa
     2641 ttcgctgaac atagaattta taacctggga gaggcaacac ggcatatcga ttggaaagta
     2701 ttcgggcgaa cagataagct gttcacaaaa aaatatgaag aggaaacaaa tctgcgctgt
     2761 catattgtga tcgacgcatc ctccagtatg tatttcccgg aaccggataa acaggcgaag
     2821 ggtttgcacc ttaataaact gatattttca gcaatttgtt cttctgccat catccatttg
     2881 ctgaaaatgc aacgcgatgc agcaggactt acaatatttg cagatgatgt gctcttgcat
     2941 accaaagcag gaagtagtaa tgtgcatcac aaaatgctcc agacgcaatt ggaaagtttt
     3001 attggcaaat cttcacatga tctgccgaca aatgccggta aatgtttgca ccaaatcgcc
     3061 gaaaatattc accgccgcag catggtggta atttttagtg atatgatgga agatgttgaa
     3121 aaatctgaag agttgttttc tgcactgcaa catttaaagc ataataaaca cgaagtgatt
     3181 ctctttcatg ttacagataa gcgacatgaa attaattttg agtacgataa tcgaccatac
     3241 atctttgtcg atatggagac cggtgaaaaa attaaagtgc gcagtagcga agtaaaggaa
     3301 aaatacaccg agcaaatggc caagtatatg caacaactga aattgcgttg cggtcaatat
     3361 ggagtcgacc tcattgaagc tgatatgatg gaggattttc gtgatgtatt gttgccttat
     3421 ttggttagaa gaggtaagat gcattgaggc tataaaggtt tcaagattag tatgcttaaa
     3481 ggtccgaaga ttcaaagatt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 gaggctataa aggtttcaag attagtatgc ttaaaggtcc gaagattcaa agatttgaag
     3661 attcgaaggg tatagggatt agtaaattag tatgttttag ggtgtgaaga ttcaaagggt
     3721 ttcggggttt gaagctgaga gggtttgatg gtaaaaggtg tgggttttgt ggggtggagc
     3781 ggctgtcatt tagatttatt taatctatga attggcgtcg aacatttgta aattgtttat
     3841 gattgcaacc attgacttta aggtaatagt gatcagtttg taagttaaat gcttataagc
     3901 gggttgttcc attttcatag aatatgtttg tgttgatatt catatggaac gcgatcttgt
     3961 taaacgaatt cgaaactttg catacaatgt ttacagacat ctaaaaagtg taagactgaa
     4021 tttttacaac cgacaaatta tgcagcagtt gttggatagc tcatttagtg cagcagccaa
     4081 ttatcgcgcc gcgcagaggg cgaaatcaag aaaagacgca ctgaataaaa ttaaaattgc
     4141 ccttgaagaa atggatgaat cgaacttctg gataacctct ctttatgatc ttgacgagtc
     4201 cacatcttct gatatgcaag ccttaatcaa agaaagcaac gaattaacca gcatactagt
     4261 agcaataata tccaaaatgg gctaagcttc aatagtaatc aacacccaac aactttccac
     4321 taattcctgc tttcaaatcc taaaatcttc aataaaacaa tctacttaac cccatcaacc
     4381 cttcgaatct tcaaatctac agcccttcga atcttcaatc cttcaaatct tcaacccttc
     4441 gaatcttcgc accttcaaat cttcaatcct tcgaatcttc aaatcttcag cccttcgaat
     4501 cttcgcacct tcaaatcttc agcccttgga ctcttcaacc cttcaaatct tcgactcttc
     4561 aacccttcaa atcctcaacc tttacttttg cctattttcg cactatcatt aggacaactt
     4621 tgaaatcagg atatttatct aacagcgcat ggaattgaat tataagctac tcggcaaagg
     4681 taaatctgtg gtcatcttgc atggattatt tggcatgctt gacaattgga taacattcgg
     4741 taaatcatta tctgaaaatt atgctgtatt cctaattgat cagcgtaatc atggcaattc
     4801 tccgcattca gagaatcata gttatacctt gatggcggaa gatgtttatc agttttgcct
     4861 caaacatcaa cttcatcaaa tcacgctcat cggccattcc atgggaggga aaactgctat
     4921 gaagtttgca ttgatgtatc ctgaactggt tgaaacactg attgtgatcg atattggggt
     4981 gaaacaatat agtggaggac atgaaactat ttttcgcgcc ttgcggcaaa tggaccttga
     5041 cgcaatacat tcaagatcgg aggctgaatc atttttaagt gaggcgataa cggaagttgg
     5101 tgtgaggcag tttttactca agaaccttac ccgcaatacc tccgggaaat atcattggaa
     5161 atttaatctg caagcacttt tcgagcatta caatgaaacg cttgcgccga ttacatcaac
     5221 gcatactttc gacgggaaaa ctctttttat tcgtggtgga aattccggat atattcagga
     5281 caaggattgg gatgatatca caactttatt tcctttggca caacttcata caattgcgaa
     5341 cgccggccac tgggtgcatg ccgatcaacc gcaattgttg cttgacctgg ttaggaataa
     5401 aattgcataa aaaaaggctg tcagtttgcg acagcctttt tttagtttgt tttacaacgt
     5461 tccacgcaat tcctgctcgc gttctatagc ctcaaaaaga gctttaaaat tgcctttacc
     5521 aaaactgcgg gcaccatgac gttgaataat ctcgtaaaat acagtagggc gatcttcaac
     5581 aggcttcgta aataattgca ataaatatcc ttcatcatcg cgatcaatga ggatattgag
     5641 ttttttcaac tcatcaaaat cttcatcaac atgtcccacg cgatctaata catcttcata
     5701 atatgtttca ggcacataaa gaaactctac accacgcgca cgcaactctg taactgtttc
     5761 gatgatatta tctgttgcaa tggcaatatg ttgaacaccg gctcctttat agaaatcgat
     5821 atattcttct atttgcgatt ttttcttgcc ttcagccggt tcgttgattg ggaattttac
     5881 atatccgtta ccattactaa ctaccttact cattagtgct gaatactcag ttgaaatatc
     5941 actgtcgtca aatgtgatca ataatttaaa tcccatcaca tcttcgtaaa atttcaccca
     6001 atcattcatg cgacccaact ctacatttcc cacacaatga tctacatatt tcaacccaac
     6061 agatgtaaca ggaacatttg atgatttcgg ctgataaccc ggcatgaata caccattata
     6121 gtttttgcgt tcaacaaatt tgtgaatagt atcgccataa gtttgaatgg cagcaactac
     6181 tacttcgccg tgttcgtctt tcatgcgcgt tggctccatt acacctatag ctccccgctt
     6241 agtagtttcg gcataactct ttgcggcatc atctacccat aaagccaaaa ctttcacacc
     6301 gtcgccatgc ttacgtacgt gttcagaaat ctcactcgaa ggatcgaaag aggtagttaa
     6361 taccaggcga attttaccct gctgcaatac ataactcgcg cgatctttca cacccgtttc
     6421 taatccggca tatgcaacta actcatatcc ccaagccgat tggtagtaat atgccgattg
     6481 cttggcatta cctacataaa attctatatg atcggtgcca ttcaaaggca aaaaatcttc
     6541 aacaagattt tttcctgttg ttaatgttat ggtatccatt ttttatgttt atcgttcaat
     6601 ttataaaata ctgaaattga aatcactgct caaaatataa cctttaatac ttgtgtgcaa
     6661 gtatgcaagt gtgcaagtgt gcagctttgt ttatttctaa gttgtgagtg atttttttga
     6721 ggttataatt attcggccta taattttgat cattggttct aattcttcta gcaagtcctg
     6781 gcaatccgga taattaggtg attggaggca aagtctcaac cagtattccg tttcgcttgc
     6841 ttctttagat gcaattttca ttttatgaat aaaatcgctt ttgctttcag cattctgagc
     6901 ttccgcaaca ttggcgccaa ctgaagttcc acacctgaat agttgatagg acatattgaa
     6961 ccgcctgtta ttttctaaaa catagcaata ttcaataatg cgcagagaaa attggaatga
     7021 tctttttaca ataccattct caggatgaaa ttgatttttc attttaattt gattttaagg
     7081 tttaaaaaaa tctcattctc tccggatcaa catctcccat aacccaaatc aaacaagttc
     7141 gtgaattgca accattcatc cacctaatca aagaacaatc aattaaacaa cagcacaaat
     7201 cttcactact ctaattcaaa ccacatacat ttcagcatat catatattta tcctcctact
     7261 cac
//
LOCUS       DAOQHY010000153         7210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_5451_length_7210_cov_8.128442, whole genome shotgun sequence.
ACCESSION   DAOQHY010000153 DAOQHY010000000
VERSION     DAOQHY010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7210
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5451_length_7210_cov_8.128442"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PLL28_11490"
     CDS             complement(<1..605)
                     /locus_tag="PLL28_11490"
                     /inference="COORDINATES: protein motif:HMM:NF024884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HNF69991.1"
                     /translation="MKHFSTRKLPILAQYAICIGVVILIAAICFSFTGIVGYRVIALV
                     LLMVVSFLAMLFEVYPVLVAAILSAVIWNFMFIPPIYTFHIDNTEDVLMFLLYFIIAS
                     VNAVLTIKIREAETKARDKEEKENTIKLYNTLLNSLSHELRTPISAILGAVDTLQEKD
                     SNLSEQNKSVLLSEIDTAGMRLNRQVDNLLNMSRLESGMLMP"
     gene            complement(703..1134)
                     /locus_tag="PLL28_11495"
     CDS             complement(703..1134)
                     /locus_tag="PLL28_11495"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNF69992.1"
                     /translation="MLSVSCKAAIKAVVFLGVQHQSSERYSIGEIAAEINENSHTVGK
                     LLQKLTHAGIINSIKGPHGGFYISEQQRHKHVIDIVEAIDGKEVFLECGLGFNKCSDS
                     KPCPFHNDFKKVRDLFTEMCRKKKICELDESVIRGNAYLRG"
     gene            1545..2642
                     /locus_tag="PLL28_11500"
     CDS             1545..2642
                     /locus_tag="PLL28_11500"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69993.1"
                     /translation="MAPQLETSDPNLDYYYDFTQSIEEYTKTFRQLQIQWQWQPVNMG
                     NYQNVIADIAAERNAGLSFPVVFNICDGDEVNGAPGVSVVHALEKHKLIYTGADAYFY
                     DITTSKITMKEAFDLAGVPTAKWEAVTDRNQDISGIFQRLGSPLIVKPAVSGGSMGLN
                     VKNVVENETELKEQINKIFDGYRGWQLSAGGIVVEQYINGPEYTTMIIGASDQPRNCV
                     IYTPVERVFHPSLPDKEKFLSFDRLWEIYEDETPMPENDNFYNYTLPARSLISKLKRI
                     SWDAYVSVGGKGYTRVDLRMDKHTGEIGVLEVNAQCGLSEDEDYTSIGAILRMSNKSF
                     AQMVVEIINDATYRWMKKNRVRKLQKSYREA"
     gene            2685..4127
                     /locus_tag="PLL28_11505"
     CDS             2685..4127
                     /locus_tag="PLL28_11505"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNF69994.1"
                     /translation="MSSVAPFKVCVLQPDYSTSDVDYQYYDPPRNLQPLLPNAIVDHV
                     FLNKLTTYKQLRELKKKGYDIYVNLCEGYLDWSIPSIDVIYCMELLGLPFTGPSSLLY
                     DPTKELMKYVAFTCGVKTPAFVLLQNLDEIDSIASQLQFPLFVKPSKAGDSLGVDEKS
                     LVHDVTALKKKAESIIEEYGPLLVEEYIAGREFTALVIASPDENGAAKVLQPIEYIFP
                     EGFAFKTYALKTSELHPNANIPVTDAAIIKKLQDATRLIFTGFNGVGYARLDFRMDDK
                     GDLYFLEINFTCSVFYSDGYEGSADYILREDGIGQEGFLRAIIDEGMARYRRTHKPFC
                     LKGNARTGYGIYATRDIEKGEVIFHGEEASQRITTRRHVETHWPADMQLVFRRYAYPI
                     SNEVFLLWDNDPNAWAPQNHSCEPSTAYDGLDVVALRNISCGEELTLDYATFLDEHME
                     PFECHCGSKNCRGLIKGTLGNSISEMERKK"
     gene            complement(4217..4774)
                     /locus_tag="PLL28_11510"
     CDS             complement(4217..4774)
                     /locus_tag="PLL28_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69995.1"
                     /translation="METPIQSIPPIDRFEPGSFSTTKSRPFPPHVFVVVFIIVVGMFL
                     NMTSPIPALLLAMVDFGLLSMRSGVEVLINAKSYSDFTSIYGIKFGKWKSLGEYDFIL
                     ITRINKRRNAMMNIAYGVSYGDVFFDMDLITKGDRDVLLNRLGEREIVIDKAIQIGQY
                     FDLRVVERVDGDYQVVFEPEKKVVK"
     gene            complement(4774..5604)
                     /locus_tag="PLL28_11515"
     CDS             complement(4774..5604)
                     /locus_tag="PLL28_11515"
                     /inference="COORDINATES: protein motif:HMM:NF039362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HNF69996.1"
                     /translation="MNTFDRMGNGWKISMAGFSVIRKNKQLLVFPILSGAVMLLIIAT
                     FIFAMLGSDFGQVQHLLDNGNPMRYFILFVYYLISYFVVVFFNMALIHCARLYFQGKT
                     PTISDGLNFSMSRIGVIFSWALVAATVGLILRSIQEKVGIVGKIITGLVGLVWSVATF
                     FVVPVLAYENLRPIDAVKRSAQIMREKWGESIGAGFSLLVFNLLGLLLVAVPLFFIGS
                     LISTLASMILAFLGVLIVTTISSAAQTIFISAAYLKINNEEIEGFDDDTIDGLFYRKD
                     "
     assembly_gap    6394..6493
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6675..7058
                     /locus_tag="PLL28_11520"
     CDS             6675..7058
                     /locus_tag="PLL28_11520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNF69997.1"
                     /translation="MMIGELRYKDISEKIIGASFEVHKFLGNGFQEVIYQRALAYEMR
                     KAGLEFAREIEQDIYYKELEVPIGTRRADFVVEGKILVELKAIIQLEDVHLAQALNYL
                     KAYKLEVGLLINFGSKSLTFKRLVL"
BASE COUNT         2318 a         1345 c         1402 g         2045 t
ORIGIN      
        1 ggcatgagca taccactttc cagtcggctc atgtttaata aattatctac ctgcctattt
       61 aagcgcatgc ctgcagtatc tatttcactc aataaaacag atttattttg ttcactgaga
      121 tttgaatctt tttcctgtaa ggtatctact gcccctaata ttgccgaaat tggcgtgcgc
      181 aattcatgag ataaagaatt taataatgtg ttataaagct tgatggtatt ttctttttct
      241 tccttatcac gtgcctttgt ttcagcttcc ctgattttga tggtaagcac tgcatttacg
      301 gaggcgatga taaaatataa cagaaacatc aacacatctt ctgtattgtc gatgtgaaat
      361 gtgtaaatag gtggaatgaa catgaagttc catataactg cactcagaat tgcggctacc
      421 agcacaggat atacttcgaa caacatggcc aggaagctca ccaccatgag caaaaccagt
      481 gcaattaccc gatagccgac aatacctgta aagctgaaac aaattgcggc aatgagaatc
      541 accacaccta tgcaaatggc atattgtgca aggataggaa gttttcgtgt gctgaaatgc
      601 ttcatcgatg tgcagaatcg atcaaaagta tggtatgcac tataaagata atataaaatc
      661 ttttgcagaa tagaagcaac tgtgatctac acattaaaca tgttatcccc tcaaataagc
      721 atttcctcga attaccgatt cgtcgagttc acatattttt ttcttgcgac acatttctgt
      781 gaacagatca cgcactttct tgaagtcgtt atgaaaagga caaggtttcg aatctgaaca
      841 tttattaaat cctaatccgc attctaaaaa aacttccttt ccatcaatag cttctactat
      901 atcgataacg tgtttatgcc tttgttgttc gctgatataa aatcctccgt gcggaccttt
      961 gatgctgttg atgatgccgg catgcgtgag tttttgcaac agcttgccta cggtatgact
     1021 attctcattt atttccgcag caatttctcc aatactgtat cgttcgctag attgatgttg
     1081 aacacccaga aatactactg ctttaattgc tgccttacat gatacactca gcattgcata
     1141 aacttttata agatgtttgt gaccttagaa gcacccgata cattatttaa accaaagata
     1201 tggattgtag tataattaaa gtattcgctt actttatttt tgcaaatcgc tttataatgc
     1261 attgataatt aaacaattat actaacacat agccaagttt ggcaaaaaat caggttatag
     1321 tccaaaagcc aacggcaaag gtaagcaagc catccaaatt tagtagtcgg atatttttca
     1381 ttatccacct caataatctt gcatggatac atacttaatg tatctttata tcaaccaaaa
     1441 cattacccca ctgcatggag caggagcaac agttggcccc gaccgccgac ccccgatttt
     1501 tactacatga tgccctaccc ttagaccaac taaaagtttg ggttttggca ccgcaacttg
     1561 aaacatcaga tcctaacctg gattactatt atgatttcac ccaaagtata gaagaataca
     1621 cgaagacttt tagacagttg caaattcaat ggcaatggca gccggtgaat atgggtaatt
     1681 accaaaatgt gattgccgat attgcagcgg agagaaatgc gggtttatca tttccggtag
     1741 tgtttaatat ttgtgatggc gatgaagtaa atggtgcacc gggcgtatcg gtggtgcatg
     1801 cattagaaaa gcacaaactc atttataccg gcgccgatgc atatttctac gatattacga
     1861 cttccaaaat caccatgaag gaggcgtttg atcttgcagg agtgcctact gccaaatggg
     1921 aagcggtaac agatcgcaat caggacatca gcggcatatt tcagcgatta ggatctccgc
     1981 tgatcgtaaa gccagcagtt tctggcggga gcatgggatt gaatgtaaaa aatgtggtag
     2041 agaatgaaac agagttgaaa gagcaaatca ataaaatatt tgatggctat cgcggatggc
     2101 aattatcggc cggaggcatt gttgttgagc aatatatcaa tgggccggaa tacaccacca
     2161 tgatcattgg cgcttctgac caaccacgaa attgtgtgat ttatacaccg gttgaacgcg
     2221 tatttcatcc atcgctccca gataaggaaa aatttctctc tttcgacagg ctatgggaaa
     2281 tatatgaaga cgaaacaccc atgccggaaa acgataattt ctataattac accttaccgg
     2341 cacgttcgct catcagtaaa ttgaaacgca tcagttggga tgcctatgta tcggttggcg
     2401 gcaagggtta tacgcgggta gacctgcgca tggacaagca taccggtgaa attggcgtgc
     2461 tggaagtgaa cgcacaatgc ggtttaagtg aggatgaaga ttatacttcc attggtgcga
     2521 ttttgcgcat gagtaataaa tcatttgctc aaatggtagt tgagatcatc aatgatgcca
     2581 catatcgttg gatgaaaaag aatcgcgtta gaaaattgca gaagtcatat cgcgaagcat
     2641 aaaaaaatca ataaataagc atcattataa atcggttgaa tttcatgagc agtgtagcgc
     2701 cattcaaagt atgtgttttg caaccggatt attcaaccag cgatgtagat tatcaatact
     2761 acgatccccc tcgcaatctt caaccgctat tgccaaatgc aattgtagat cacgtttttt
     2821 taaataagct tactacctac aagcagttac gtgaacttaa gaaaaagggc tacgatattt
     2881 atgtaaacct gtgtgaaggt tatttagatt ggagtattcc gtcaattgat gtgatttatt
     2941 gcatggaatt gctgggcttg ccttttaccg gaccttcatc attactctac gatcctacca
     3001 aggagttaat gaaatatgta gcatttacat gcggtgtaaa aacgccagca tttgtattat
     3061 tacaaaacct tgatgagata gattccattg catcacaact tcagtttcct ttatttgtaa
     3121 aaccatcaaa agcaggagat agtttggggg ttgatgaaaa gtcactggtg catgatgtaa
     3181 cagcattaaa gaaaaaggcc gaatcaatta ttgaagaata cggtcctttg ttggtggaag
     3241 aatatattgc cggcagagag tttacagcac tggtaatagc ctcacccgat gaaaatggcg
     3301 ctgcaaaggt tttacaaccc atagagtata tttttcccga aggatttgct ttcaaaacct
     3361 atgcgctgaa gacatcagaa ttacatccta acgccaatat acctgtcacg gatgctgcta
     3421 tcattaaaaa gttgcaagac gccactcgct taatatttac aggcttcaac ggagttggtt
     3481 acgcccgatt agatttcaga atggatgata aaggcgacct ttattttttg gagatcaatt
     3541 tcacctgttc cgttttttat agcgacggat atgagggatc agcagattat attttgcgcg
     3601 aagacggtat tggacaggaa ggttttttac gtgctattat tgatgaaggg atggcgcgtt
     3661 atcgtcgaac acataaacca ttttgtttaa aaggaaatgc gcgcacagga tatggtattt
     3721 atgcgacacg tgatattgag aagggtgaag tgatttttca cggtgaagaa gcatcacagc
     3781 gcatcaccac gcggcgacac gtagaaaccc attggcccgc cgacatgcag ttagtattca
     3841 gaagatatgc ctatccgata agcaatgaag tgtttttatt gtgggataat gatcccaatg
     3901 cctgggcgcc tcaaaatcac agttgcgagc catcaacagc atacgacggt ttggatgttg
     3961 tagcactgcg caatatttca tgtggtgaag aacttacact tgattacgct acatttttag
     4021 atgagcacat ggaaccgttt gaatgccatt gcggtagcaa gaattgccga gggttaatca
     4081 aaggaacttt aggaaacagt atttctgaga tggagagaaa gaaataaaaa gtgatggcaa
     4141 tatatagtgt atgcgaattt tggcagaaca gaaaaaatta aactgcgttt tgtttcagct
     4201 gtcataaaat tttgcattat ttgaccacct tcttttccgg ttcaaaaact acctgataat
     4261 ctccatccac tctttccaca acacgtaaat cgaaatattg accaatttgg attgccttat
     4321 caattacaat ttctctttcg cccaagcgat ttaacaacac gtctctatca ccttttgtaa
     4381 tcaaatccat atcgaagaaa acatcaccat aagaaacgcc atacgctata ttcatcattg
     4441 cgtttctacg tttattaatg cgggtaatca gtatgaaatc atattcacct aaagatttcc
     4501 atttaccgaa tttaatccca tagatgctgg taaaatcact gtatgatttt gcattaatca
     4561 acacctctac acccgatcgc atagataata atccgaaatc taccattgca agtaaaagcg
     4621 ccggtatcgg gctagtcata ttgaggaaca ttccaactac aataataaag accaccacaa
     4681 aaacatgcgg tggaaagggc cttgacttcg ttgtggaaaa cgagccgggt tcaaaacgat
     4741 ctattggagg aatagattga attggtgttt ccattagtcc ttgcggtaaa acaatccgtc
     4801 aatcgtatca tcatcaaagc cttctatctc ttcattgttg atttttaaat aagcggcgct
     4861 gataaaaatt gtttgtgcag cgctacttat tgtggtaaca attagaacac caagaaaagc
     4921 gagtatcatg gaagcaagtg tgcttattag cgagccgata aagaataaag gtacagctac
     4981 caataataag ccgagaagat taaatacaag taaactaaaa ccggcaccaa tgctttcacc
     5041 ccatttttcg cgcataattt gagcagaacg ctttactgca tcaatagggc gcaaattttc
     5101 atatgccagc accgggacca caaaaaatgt agcaacactc catacaaggc caacaagacc
     5161 ggtaattatc ttacctacta tgcctacctt ttcctgaatt gatcgcaaaa tcaaaccaac
     5221 cgtcgccgct accaaggccc aagaaaaaat cacccctatg cggctcatgc tgaaattaag
     5281 tccatcggaa attgttggtg tcttaccttg aaaataaagg cgggcacagt gaattaatgc
     5341 catgttgaag aacacaacta caaaatagct aatgaggtaa taaacaaaca aaataaaata
     5401 tcgcattgga ttaccattgt cgagcaggtg ttgtacttgc ccgaaatcgg aacccaacat
     5461 tgcgaatata aaagtggcga tgatcaataa cattaccgct cctgaaagga ttggaaaaac
     5521 taataattgt ttgtttttgc gaattaccga aaaccctgcc atcgagattt tccagccatt
     5581 gcccatgcgg tcgaatgtgt tcatgtgatt gaattttata taaaattagt aaaaatggtt
     5641 tacaaataca atatggagag attacgggaa ttcgcgagaa ggaccggaag gggtggaaat
     5701 atagggtgat gacaacttac aaattcgtca attcgatagt gaaattcgct aggccgggaa
     5761 ggtttaagca cgtcaagtta taggaagaaa tgtatggcca tcactaaatt aaattggaat
     5821 ttgattaaaa aatccgtgtg caattgttgt ctgaatcacg gattacacag atttagagat
     5881 tacacagatt taaagattat acagatttag agattagacg gattttttta actaattaga
     5941 ggtggatttc taatgaaaat tatttttatt gagaagacat gcaaaaaatt gcacattaac
     6001 atcatttgaa taattcatta gtgcgcgaag gtctgaatgc ttagaccgct cccctataaa
     6061 ttcttaaatc cgtgaaatcc attaatccgt ccaatccgtg attcagacat ctttaaaagc
     6121 aatggccggc ttctaagtat ctcacttgaa aaccggagcg aaaaaacact cctgcttgcg
     6181 ctcaagatca catgcgcata tgaacacacg aacaaaataa ataatgtgca attattgtct
     6241 gaatcacgga ttacacagat ttaaagatta tgcagattta aagattatac agatttagag
     6301 attatacaga tttagagatt agacggattt ttttaaccaa ttagaggtgg atttctaatg
     6361 aaaattattt ttattgagaa gacatgcaaa aaannnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnntgaacac acgaacaaaa taaataatgt gcaattattg tctgaatcac
     6541 ggattacaag gatttagaga ttacacagat ttaaagatta gacggatttt ttaagactaa
     6601 ttgtatgtat acttgcagat ttatattgaa tatgaaatta tttagtaaat aaattatatt
     6661 taaattttat ttgcatgatg attggagaat tgaggtataa ggacatttcg gagaaaatca
     6721 ttggtgcttc gtttgaagtg cataaattct tgggcaacgg ctttcaagag gtcatttacc
     6781 aaagggcttt agcgtatgaa atgcgcaaag cgggattgga atttgccaga gagattgaac
     6841 aggatattta ttacaaggaa ttggaagttc ccataggcac acgcagggct gattttgtgg
     6901 ttgagggcaa aatcttggtt gaactcaaag caattattca gctagaagat gtgcatctag
     6961 ctcaagccct aaactacctt aaggcctaca aactagaagt aggactactc atcaacttcg
     7021 gcagtaaaag tttaaccttt aagcgcttag tgttatgaat agaacaattg aatatcaaat
     7081 accgaaaacc tcactctaca acaatcgaaa caaatccatg taatgcatta atccgtgaaa
     7141 tccattaatc cgtctaatcc gtgattcaga catcttcggc agtaaaagtt taacctttaa
     7201 gcgcttagtg
//
LOCUS       DAOQHY010000154         7191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_5478_length_7191_cov_4.976598, whole genome shotgun sequence.
ACCESSION   DAOQHY010000154 DAOQHY010000000
VERSION     DAOQHY010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7191
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5478_length_7191_cov_4.976598"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="PLL28_11525"
     CDS             <1..1046
                     /locus_tag="PLL28_11525"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HNF69998.1"
                     /translation="LKKQHPQAYIVLSFYSPSGFEVRKDYAGADVVTYMPLDTAANAS
                     RFIDIIKPDVAIFVKYEFWYHHLKALKSRNIPTYLASGIFRKGQLFFKWYGRLHKDML
                     RCFTTLFVQEEGSLALLEKIKMTHGVLAFDSRFDRVKEIADKPVSIPLIAQFVEGFNV
                     LVAGSTWPKDERVLQRVFYKSLVFVNFKLILAPHHIEGKWMQRTMKLFKKFSVKYSEA
                     EKLPYEELMAKRIMIIDNMGMLSQLYRYADVNYIGGGLNKGVHNTLEAAVYGKPLIIG
                     PRYKKFHEAVGLVKANAALVITDADDLLNRINLMNQFPFVWKGVGIDAAKYVTDRLGG
                     TETMADIISKKIS"
     gene            complement(1047..1943)
                     /locus_tag="PLL28_11530"
     CDS             complement(1047..1943)
                     /locus_tag="PLL28_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF69999.1"
                     /translation="MEIALPKFKTTDLPMDAKITRVEVLLDKLNMVFAEVKSDGSLDP
                     LELELLRKYTRQLAETLDLPLEAGHNITPVTTIPPVEKIQEVNLPPAPVEIPVEPIMV
                     KTEEVIMKIVADDEIETPIVIKPKIETVPVVEEKLPVVEKEIIVEPVKPVLEENPVKE
                     IIPPAAEKQEELPPAKKETPVKKPLVKSVESDDDEDEQTAGLSDKLQTGNKKTLADKI
                     SAHKAKDLKSVIPLNDKLLITKSLFGGDKDAYEHTIKALNNMQSLTSAEQFIHGELKS
                     KFNWQDEDAIERLIEMVQLKFS"
     gene            1967..2536
                     /locus_tag="PLL28_11535"
     CDS             1967..2536
                     /locus_tag="PLL28_11535"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNF70000.1"
                     /translation="MFIEPHLKGEGRGWIEVICGSMFSGKTEELIRRLRRARIANLKV
                     EIFKPTIDTRYDAVNIVSHDDTQIVSTPVANSLNILLMADDTEVVGIDEAQFFDEQIV
                     EVCEKLAQRGTRVIVAGLDMDYRGKPFGPMPQLLAIADYITKVHAICVQCGNIASYSY
                     RKTTDKSQVLLGEKNEYEPRCRSCFLKES"
     gene            2636..4228
                     /locus_tag="PLL28_11540"
     CDS             2636..4228
                     /locus_tag="PLL28_11540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70001.1"
                     /translation="MKKNLVLITSLFIYTVMQAQAPLNSPNLLLPGTDLIRDAKTVTK
                     ADLHKATLDEGDRALNYYWVNYSDAVDFAYNGWALENLASLPLWPDSTALVVTGDGSD
                     FYWYGHGYAHMFDPISDYISDFVDENYTVIGNASDWWNEDHSFKIDSVRFYYFYDRFN
                     TSYTDTLKVYVMAPNSTCYQEGFYLDNDASGDFDEGIDISLFMSKYNYVNNRPSGTYT
                     EYTFLLGDDDTASFNIASLNIPLALTINKNSPDHNVGIAWQFIPGQPYSTGDTLLDFS
                     DPPLLVTNPLNRFWLLCNEEILESDPASWSEYSNNQDGFASTEVRYNISDGGWNGYYV
                     STYAYVDAFAFEHAYVDWYVAPRGVNFLATDPSPCVSLEKNFTDLSNFAAVPDDATYY
                     WEFGDGAVSFDRNPVHTYPIPGSFEVCLSVDYGGEAFDYCKNVAVDYCTAIEDIENLT
                     SFSLYPNPADDVLNVNLTFGVAQQATIEIVNMAGQVMITENVNATVNYNTSMDVSSLP
                     AGIYMVRINSGIQSSVRTFVVE"
     gene            complement(4758..>7191)
                     /locus_tag="PLL28_11545"
     CDS             complement(4758..>7191)
                     /locus_tag="PLL28_11545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70002.1"
                     /translation="PGTFMWPTMTATGDGVFKAPAVTGGALPTFSQLTATIPTPGEVG
                     SNSSAAWAYVNRIACHPTDNNIVAAATNTGLKVSTDGGNTWSNAEGGGVPLSGITDDV
                     KFDADGYLHAVSGSLRKYYRSTTAADPATLDELGAGLPTGSTRRALAVAPSDPNYVYI
                     YSAKSGTYGCQGIFQSADHGANFIQISEEASEFFNPNGTSANITWNVCIAVNPADAQR
                     IYIGGQIQSWTWDGASGSWTPMTSSGYPTWYPKYIHADQHYITFPADYNPDTKDIMYF
                     CSDGGIARSLNASSQYPDFGTLNKGLNFYQSHGVASGINGEVMGGAQDNGTHLVDFQQ
                     NSEFQGFEWLGGDGGRVEISKIRPEYLFGTFFYVGASGNGAALRRSVNSGSSASNMYD
                     PNIDDDQDGLADGGTDFVTTFVMWENYKLYETFKDVLVGGTVEYPAGSGNFYVEGDEV
                     NYEGRTITLTRTGLSESRFYHAVKNKLWMTTGALFNSTEAPEWFKVIPVTSGNISAIE
                     YDATGDIVYVGTESGRLYRLDGMLDAAYEYVDTDGDPGTAGVFAPADAGITQFTYANV
                     FPGKITGISIDRENPDQIAISIGGFGVDQNVWTSANALDGDAAVFNCVSDAGSLPNIP
                     VYDILVHAADPDKLLIGTEFGVWSYSISGGGSWTQENGAVGGGVGPGNVPVFEIREDF
                     VRETDCMAIYMGTHGNGYYRTTTLAAGDCDFSTVSAGPIQEEIIAGITIAPNPASYTT
                     TATMTLERNASSVTISLVNLMGATVRNYGTSAYNEGTHNINLDLKGITPGTYLVVFNI
                     DGVVISRRLVVM"
BASE COUNT         1951 a         1581 c         1582 g         2077 t
ORIGIN      
        1 ccttgaaaaa gcaacatcca caagcatata tcgtattaag tttttattct ccttccggat
       61 ttgaagtcag aaaagattat gcgggtgccg atgttgttac ttacatgcca ttagataccg
      121 cagcaaatgc ttcgcgattt atagatatca tcaaacctga tgttgccatt tttgtgaaat
      181 acgaattctg gtatcatcac ttaaaagcgt tgaaaagcag gaatattcca acctatctcg
      241 cttcaggtat tttccgcaaa ggacaattat tctttaaatg gtatggtaga ttgcacaaag
      301 acatgttgcg ctgcttcacc actttatttg tgcaggaaga aggctcactg gcattgttgg
      361 aaaaaattaa aatgacacat ggcgtacttg ccttcgacag tcgatttgat cgcgtgaaag
      421 agattgccga caagccggtt tcaattcctt tgattgcaca atttgttgaa ggatttaatg
      481 tgttggtggc aggtagcacc tggccgaaag atgagcgtgt acttcaaaga gtattttata
      541 aatcactcgt atttgtaaat ttcaagctga tattggcgcc acatcatatt gaaggcaagt
      601 ggatgcagcg cacgatgaaa ttatttaaga agttctcggt aaaatattca gaagccgaaa
      661 aattacctta tgaagaattg atggcaaaac gcattatgat catcgacaat atgggcatgc
      721 tgtcgcagtt gtatcgttat gccgatgtta attatatcgg aggtggatta aataagggcg
      781 tccacaatac attggaagct gctgtatatg gtaaaccgct cattattggt ccgcgataca
      841 aaaaatttca tgaggccgtt ggtttagtaa aagccaatgc cgcattagtg attaccgatg
      901 ccgatgatct gctgaaccgc atcaatttga tgaatcagtt tccatttgta tggaaaggcg
      961 ttggtataga cgctgcaaaa tacgttaccg accgtctagg cggaactgaa acgatggctg
     1021 acattatcag caagaaaatt tcgtgatcag ctgaatttca attgcaccat ttcaatcaat
     1081 cgctcaatgg cgtcttcatc ctgccaattg aatttcgatt tcaattcccc gtgaataaac
     1141 tgttcggctg aggtcaagga ctgcatatta ttaagcgcct tgatggtatg ttcataggca
     1201 tccttatcgc ctccaaataa ggatttcgta attagcaatt tatcattcaa tgggatcacg
     1261 cttttcaaat cctttgcttt atgcgcgctg attttatctg caagtgtttt tttattgcct
     1321 gtttgtaatt tatcgctcag tccggcagtc tgctcatctt catcatcatc gctctctacc
     1381 gatttcacca atggcttttt aacaggtgtt tctttttttg ccggaggcag ctcttcctgt
     1441 ttttctgctg caggtggaat aatttccttc acaggatttt cttcaagaac aggttttacc
     1501 ggttcaacaa ttatttcctt ttcaacaaca ggaagttttt cttcgacaac aggaactgtt
     1561 tcaatttttg gtttgatgac aatgggcgtt tcaatttcat cgtctgcaac tattttcatg
     1621 attacctctt ctgttttcac cattattggc tcaacaggaa tttcaacagg tgcgggtggt
     1681 aagtttactt cttgtatctt ttctaccgga gggatggtag taacgggtgt gatattatga
     1741 ccagcttcta agggcaaatc aagtgtctcg gcaagctgac gggtatattt cctgagcaac
     1801 tccagttcca gggggtcaag agagccatca cttttcactt cggcaaacac catattcagt
     1861 ttgtcgagca acacttccac acgtgttatt ttagcatcca taggtaaatc cgtagttttg
     1921 aatttcggta aggcaatttc cataccatta aaacgagtac aaatgtatgt ttattgaacc
     1981 gcatctaaaa ggtgaagggc gtggatggat agaggtcatc tgcggatcga tgttctcggg
     2041 taaaaccgaa gagctcattc gcaggttgcg ccgggcacgt attgccaatt tgaaagtaga
     2101 gattttcaaa cccaccattg atacccgtta tgatgcagtg aatattgtat cacatgacga
     2161 cacacagatc gtttctacgc cggtagccaa ttcattgaat atattgttga tggcagatga
     2221 caccgaagtt gtaggcatag atgaagccca atttttcgat gaacaaatcg ttgaggtatg
     2281 tgaaaagctt gctcagcgcg gcacaagagt tattgttgcc ggtctcgata tggactaccg
     2341 tggcaagcca tttggtccca tgccgcaatt actggccata gccgattata tcacgaaagt
     2401 acacgctatt tgtgtgcaat gtggaaatat cgcttcctat tcctatcgca aaaccactga
     2461 taagtcgcag gttctactcg gagaaaaaaa tgaatacgaa ccgcgctgca gatcctgttt
     2521 tcttaaggag agttaagtta gcaataaagt ccgttaactt acattatcct gacctttgga
     2581 aggtggattt atgtatattt gtggatatta ttattagctc aactaaacgc atgctatgaa
     2641 aaaaaatctt gtactcatta ccagtttgtt catctacacg gttatgcagg cacaggctcc
     2701 tctcaatagc cccaatctgc tgttgccggg aaccgacctc atccgcgatg cgaaaacggt
     2761 tacaaaagct gatttgcata aggctacatt agacgaaggc gatcgcgctt tgaattatta
     2821 ttgggtgaat tactccgacg cagtagattt cgcctataac ggctgggccc ttgaaaacct
     2881 ggcttcattg cctttatggc cagattcaac agcattggtt gttaccggcg atggtagcga
     2941 cttctactgg tatggtcacg gatacgcaca catgttcgat ccgatttctg attatatctc
     3001 agattttgtt gatgaaaact ataccgtaat cggcaatgcc agcgactggt ggaatgaaga
     3061 tcacagcttc aaaatcgact cagtgcgctt ctactatttc tacgatcgct ttaacacaag
     3121 ctatactgat acacttaaag tgtatgtaat ggctccaaat tctacctgct accaagaagg
     3181 tttttacctt gataatgatg ccagcggcga cttcgacgaa ggtatcgata ttagcttgtt
     3241 catgtcaaag tataactacg taaacaaccg ccctagcgga acctatacgg aatacacctt
     3301 cttgttgggt gatgacgata ctgcaagctt caatatcgcc agcttgaata tcccattggc
     3361 gctgacaatc aacaaaaaca gccctgatca taacgtgggt attgcatggc agttcattcc
     3421 gggacaacct tacagcacag gcgacacttt gcttgacttc tctgatcctc cactactggt
     3481 taccaacccg cttaaccgct tctggttgct ttgtaacgaa gagatccttg aaagcgatcc
     3541 tgcttcctgg tcagaatatt ctaacaacca ggacggtttt gcctctacag aagttcgcta
     3601 caatatctct gatggcggat ggaatggtta ctatgtgagc acttatgctt atgttgatgc
     3661 ttttgctttc gaacatgctt atgttgattg gtatgtggct ccaagaggtg taaacttcct
     3721 ggcaacagat ccttcacctt gcgtaagcct tgaaaagaac ttcactgatc tttcaaactt
     3781 cgcagcggtt cctgatgatg ctacttacta ttgggaattt ggtgatggcg ctgtaagctt
     3841 cgatcgcaac cctgttcaca cctacccaat tcctggttca tttgaagttt gtctctctgt
     3901 agactacggc ggtgaagcct tcgattattg caaaaacgta gctgtggact attgcacggc
     3961 tatcgaagat attgagaatt tgacctcatt cagcctgtat ccaaaccctg ctgatgatgt
     4021 gttgaatgtt aacctcactt ttggtgtggc tcaacaagca actatcgaaa tcgtaaatat
     4081 ggcaggtcag gtaatgatta ctgaaaatgt aaatgcaact gttaattaca acacttctat
     4141 ggatgtaagc agtcttcctg ccggtatcta tatggtgcgt atcaacagcg gtattcaaag
     4201 ctctgtgaga actttcgtag tagaataatt ctccggatat acctctttca aagagccgat
     4261 tcattgcgaa tcggctcttt tttatttgtt gaatatcaat gcattatata acattatctg
     4321 caatcctaca gcgtcggttt tgtaatgctc cattgctatt acccctctac cctgcccgta
     4381 aataggctgt ttcaggcgaa tacagctgtt atagatggga agtcttatcg gtttacagta
     4441 tcaaatagca aacataacta actgaatatt aattgattat agacttgaac tgagggatga
     4501 aattgcattg gttgtagtgt accataccag tgaaagacgc caggcactat aaggtcatca
     4561 gggcatgtat atacccgttt cccgactgcg tccttaggtg tgattgaagc atcatttgtt
     4621 gcatttatat ttattaatgg aaacagggag gaagcacttt ttcctgccac caatacttca
     4681 agcacacacc tcgaaatgta cggcaaaaaa aagcccccgc cgtggaggca gaggcttcaa
     4741 taatatcttg ctaaggatta catcaccacg aggcggcgag atatcaccac gccatcgata
     4801 ttgaatacta ccaggtaagt tcccggagtg attcctttga ggtcgaggtt gatattatgc
     4861 gttccttcat tataggcact agttccatag tttctaacag tagcacccat gaggttcaca
     4921 agactaatcg tcactgaaga ggcattgcgt tccagtgtca tagttgccgt tgtggtataa
     4981 gatgcagggt ttggtgcgat agtaatacct gcgatgattt cttcctggat aggacctgcg
     5041 cttacggttg agaagtcgca atcaccggca gccagcgttg ttgtacgata gtaaccattt
     5101 ccatgagttc ccatgtaaat ggccatgcaa tctgtttcac gaacaaagtc ttcgcggatt
     5161 tcgaatacag gcacgttacc gggacctacg ccaccgccaa ctgcaccgtt ttcctgcgtc
     5221 cagctgccac cacctgaaat agagtagctc catacaccga attctgttcc gatgaggagt
     5281 ttatcaggat cagcggcatg aacaaggata tcataaacag gaatgttagg aaggcttcct
     5341 gcatcagaaa cgcaattaaa tacagcagca tcgccatcga gcgcgtttgc tgatgtccat
     5401 acgttttgat caacaccgaa accaccgata gagatagcta tctgatcagg attttcgcga
     5461 tcaatgctga tacctgtgat tttacccggg aaaacgtttg cataagtaaa ctgtgtgatt
     5521 ccggcatctg caggagcaaa cacgcccgct gttccgggat ctccatcagt atcaacatat
     5581 tcgtaggcag catcaagcat accatcaagg cgatacaagc gtccgctctc agttccaaca
     5641 tatactatat caccggttgc atcgtattcg atagcgctga tgttaccgct tgttacaggg
     5701 ataactttaa accattcagg agcctcagta gagttaaaga gtgcacctgt agtcatccac
     5761 agtttatttt taacagcatg gtagaaacgt gattcagaga gacctgtgcg tgtaagcgta
     5821 atggtgcgac cttcatagtt cacctcatca ccttccacat agaagtttcc gcttcctgca
     5881 ggatattcta ctgttccacc taccaataca tctttaaatg tttcgtagag tttataattc
     5941 tcccacatca cgaatgtggt tacgaagtca gttccaccat cggcgagacc atcctgatca
     6001 tcgtcgatat tagggtcata catattgctt gctgagcttc cgctgttcac agatctgcgc
     6061 agtgcagcac cgtttccaga agcgccaaca tagaagaagg ttccgaagag gtattcagga
     6121 cgtattttgc ttatttcaac acgaccacca tcgccaccta accattcgaa tccttggaat
     6181 tcgctgtttt gctggaagtc gacaaggtgt gttccgttat cctgagcacc acccattact
     6241 tcgccattga taccgctggc cacgccatgc gactggtaga agttaagacc tttattgagg
     6301 gttccgaaat cagggtactg agacgatgcg ttcaaagaac gggcaatacc gccatctgaa
     6361 cagaaataca taatgtcttt agtatccgga ttgtaatcgg cagggaaagt gatatagtgc
     6421 tgatcggcat ggatatattt aggataccag gtaggataac cggagcttgt cataggcgtc
     6481 cagctacctg aagcaccgtc ccatgtccac gattgaattt gaccaccgat ataaatacgt
     6541 tgtgcatcgg ccgggtttac tgcgatacac acgttccagg tgatgtttgc gcttgttccg
     6601 ttaggattaa agaactcgct tgcttcttca gaaatctgaa tgaagttagc accatggtcg
     6661 gcagattgga agataccctg acatccgtaa gttccggatt ttgcagagta gatgtacacg
     6721 tagttaggat ctgaaggagc cactgccagt gcacggcgag tgcttcctgt tggcagaccg
     6781 gcaccgagtt catcgagggt agcaggatca gctgcagtag tactgcgata atatttacga
     6841 aggcttcctg aaacagcgtg caggtaacca tcggcatcga attttacatc atcggtaatt
     6901 cctgaaagcg gcacgccacc accttcagca tttgaccagg tgttaccgcc gtcggtagat
     6961 actttcaaac cggtattggt agcggcagcc acgatattgt tatctgtagg gtggcaagcg
     7021 atgcggttaa cataagccca tgcagcgctt gagtttgagc caacctcacc gggagtgggg
     7081 atggttgcag tcaactgcga aaatgtaggc agagcacctc ctgtaacggc aggagctttg
     7141 aaaacgccgt cgcctgtagc tgtcatggtg ggccacatga acgtgccagg a
//
LOCUS       DAOQHY010000155         7144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_5521_length_7144_cov_4.958668, whole genome shotgun sequence.
ACCESSION   DAOQHY010000155 DAOQHY010000000
VERSION     DAOQHY010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7144
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5521_length_7144_cov_4.958668"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(291..581)
                     /locus_tag="PLL28_11550"
     CDS             complement(291..581)
                     /locus_tag="PLL28_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70003.1"
                     /translation="MQGITLFPFVIYQHPALLSKPSFINHEHIHLRQQRELLIIPFYV
                     IYLLHYLFNLIKYRRHHKAYLNIVFEREAYTHENTPNYLQSRKWMAWMIYWK"
     gene            complement(632..1441)
                     /locus_tag="PLL28_11555"
     CDS             complement(632..1441)
                     /locus_tag="PLL28_11555"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNF70004.1"
                     /translation="MKLTLTPQVTVHRTAIRSSFLNREVQLTVILPPDYRQKPYPVLW
                     LNDGQDIDPMGVADILQQAMVSNAIPQIITVGIHANEDRLQEYGSSVAADFKQRGTKA
                     GIYAEFFINELIPFIRNTYSVSRDRRKNAIAGWSMGGLSAIDIAWSHPEAFTRVGVFS
                     GSLWWRMKAYNRGYKDDKHRIAHQMIRNSEKRDGLKFWFEVGTMDEKADRNKNGLIDS
                     IDDTLDLIKELKNKGYTDKEDISFVVVKEGEHDLRTWAGVMPHFLQWAFGK"
     gene            1484..2275
                     /locus_tag="PLL28_11560"
     CDS             1484..2275
                     /locus_tag="PLL28_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HNF70005.1"
                     /translation="MFRIRVRLFSTTFACKTAITLELNEQKHTWQSSVLGIQMEMLSF
                     GTGGFPLIIFPTSMGMHNENRDFKLIESIGWFIETGMVKVYCPDSIDKHSWYNKEASP
                     ADRVLNHMLYDRMIMEEIIPGITKDSGYSRIGVAGCSFGGYHACNFAFRHPDLTAVMI
                     SLHAKFDITGQLDGYYDDNVYYNNPPDFVPGLSDEQLWKMQIFLGSAEYDMCLDANYH
                     MAHLLGTKQVPHVLEVEQGDKHDWPCWRKQLPRFLSLIQYDNLHS"
     gene            2297..3292
                     /locus_tag="PLL28_11565"
     CDS             2297..3292
                     /locus_tag="PLL28_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70006.1"
                     /translation="MKRIGIVHGMEDSFPNAFIERINSKRIDGIVAERVLIDKVVQGA
                     ASGYDVILDRISHDAPFYRAYMKNAAISGAAVMNNPFWWSADEKFFNNALAVKIGIPV
                     PNTVLLPSHDLPENTKDTSFRNLKYPLDWESIFNYIGFPAYMKPHDGGGWRGVSRLEN
                     MEEFFSKYAETGDDVMILQSEVTFDYYFRCYCMGGKYVHIMYYEPRNPHHLRYTWNGP
                     QAPQSLLDTVKTYTLALNQALGYDFNTVEWAVKDGIPYAIDFCNPAPDADLQSVGEEN
                     FEWVVEHAALMAIERALAHKPGQDNLNWGTYVRNSVGGVPHLPQTQPIFNSALSY"
     gene            3310..4416
                     /locus_tag="PLL28_11570"
     CDS             3310..4416
                     /locus_tag="PLL28_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HNF70007.1"
                     /translation="MSIYKDFTLGIEEEFQIIDPETRDLSHRKHHIIDAAAKYNVDIK
                     AEMHQSVVEAATTICKDIHDARNQVVKLRRIIADLADKDNLKIAAAGTHPFAAWEEQL
                     ITNHPRYQEIVNEFQEAARSNLIFGLHVHIGMPSRDVAVFMMNSLRYFIPHIFALSTN
                     SPFWEGRDTGFKSFRTKVFDKFPRTGIPDVFNDASHFDNFIALLVKTNCIDNGKKIWW
                     DLRVHPYFNTIEFRMCDIPMRADETIALAALMQALTVKLYKLMKSNIGWRNYDRALIQ
                     ENKWRAARYGISGKLIDFGKQEESETRGLVLELLDFVDDVVDELGSREALENVRWILD
                     NGTGADRQVKMYYEKNRDFKALADYMIDETYIGI"
     gene            complement(4495..>7144)
                     /locus_tag="PLL28_11575"
     CDS             complement(4495..>7144)
                     /locus_tag="PLL28_11575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HNF70008.1"
                     /translation="SIIFFTSDNGYLIGEHMMRAKVMPLEESIRVPLYVRYPAWFPAG
                     QFIDNDLAELIDIPKTILDAIGIPDTFGFMGKSLRDLAEPDTLRPYAFYEYEGTEPDD
                     VHHVPDIRGVRSFDYLYTTTNCDCWTEEFYDLTTDPLQDTNQVANPKYHEKILEYRAI
                     LAQLRLEKFDTLPFESGDCGLYGSYEIPDDIDNDCDGIIDNDLDAYIRYQDIDNDGYG
                     DPATSMIVFGELPGYVNNPNDCNDTSASIHPAAIEICDGIDNDCDGFIDDADPDVVGM
                     TTWYFDNDFDGLGNDTVQIVSCFAPIGYISVGGDCNDNNPAYTIGGPEICNGIDDDCD
                     GLFDDADPDITGQTIWYADNDGDGLGNDTITIIACGAPEGYVITFGDCNDSNADFTIG
                     SPEICNAYDDDCDGLVDDADPDITGQTIWYADIDNDGYGDWANTSLNCFAPEHYVAIF
                     GDCNDADSLQTHGTPEVCNLLDDDCDGLTDDEDPDVTGKILFYADMDSDGYGNITMTT
                     LNCFLPIGYVTNGLDCNDLHSGIHPLASEICDGIDNNCNLLVDDADPLITGRPTWYLD
                     ADFDTYGSALISVEACNQPVGYVDNNSDCNDLNGLVHPGVADYCDLINNDCDAFLDED
                     PVNPVITASGPVEFCKGSSVTFTASPIISGFNRQWYKGTSPIAGATGITYTATQSGSY
                     KVKYTAPAGCITYSAITTVTVNNNPKPVVSLTSASNDLCVNNPVKLSCKNKIGSTSQW
                     YKGAVPLFGEVTNKYNATTSGNYKVKQIDPTGCFGTSSAYSVVQTCHEASPYQDEADA
                     SFVIYPNPSQGDFTLQIDFGADINGEASVKIYSMLGQMMYEAFIIVDNGEIDDQLNVS
                     GQIPSGVYLIEATIDQQKLVREIVIE"
BASE COUNT         2108 a         1477 c         1502 g         2057 t
ORIGIN      
        1 actcaatacc cactactcac taccagtcgt gaatcgtgat tcgtgactcg tgagtcgtga
       61 gtcgtgattc gtgactcgtg aatttttacc tgatacttga tacctgatac ttgatacttg
      121 atacctgata cttgatacct gatacctgat acttgatacc tgatacctag tctagatact
      181 agatacccgc cagaggcgag gcgagcttgg ctctagatac tatcacaatc ctcaaaaacc
      241 cattcaacta caaacaacac caaccatatt aaaaccaatt aataaattca ctatttccaa
      301 taaatcatcc atgccatcca ttttctagat tggaggtaat tgggggtgtt ttcgtgggtg
      361 taggcttcgc gttcgaaaac gatgttgagg taggccttgt gatgcctgcg atatttaatc
      421 aggttgaata aataatgcaa gaggtagatg acataaaatg gaataatcag taattctcgt
      481 tgctgcctga ggtgaatatg ttcgtgattg ataaatgagg gcttgctgag taaagcagga
      541 tgctgataaa tgacaaatgg gaataatgta attccctgca tataaaacca cttgcagtga
      601 attaactgcc aattcttgat tccggatgta gtcattttcc aaaagcccat tgcaaaaagt
      661 gcggcatcac accggcccag gtgcgcaagt cgtgttcacc ttcttttacg acaacaaagc
      721 tgatatcctc tttatcggta taacctttgt ttttgagctc tttaatgagg tcgagtgtat
      781 cgtctataga gtctatgagt ccgtttttat tgcgatcggc tttttcgtcc atagtgccca
      841 cttcaaacca gaatttcaac ccatcgcgtt tttcggagtt gcgtatcatt tggtgggcaa
      901 ttctatgttt gtcgtcttta taacctctgt tataggcttt cattcgccac caaaggctac
      961 cggagaacac ccctaccctt gtaaacgctt ccggatgact ccaggcgatg tcaatggcac
     1021 ttagtccgcc catactccac cctgcaattg catttttcct gcgatctcgc gacacgctgt
     1081 aggtattgcg gataaaggga atcaattcat tgataaaaaa ctcggcataa atccctgcct
     1141 tggtgccacg ctgtttgaaa tctgcggcaa cactgcttcc gtattcctga agacggtctt
     1201 cattggcgtg gatgcctact gtgatgatct gcggaatagc attagaaacc attgcctgct
     1261 gcaggatatc tgcaacaccc atagggtcta tatcctgtcc gtcgttgagc cacaacacag
     1321 gatagggctt ttggcggtaa tcgggaggta gaatcactgt gagctgcact tccctgttca
     1381 ggaacgacga cctgatagca gttctatgaa ccgtaacctg aggagtaagt gtcaacttca
     1441 tgtatagacg caaatttaat gtaaacaaca caactctgtg cacttgtttc gcattcgtgt
     1501 tcgcttgttt tcaactacct ttgcgtgcaa aactgcaatc actttggaac tgaatgaaca
     1561 aaagcatacc tggcaatctt cggtgcttgg tatacagatg gaaatgttga gttttggaac
     1621 cggcggattt cctttgatca tattccctac atcgatgggc atgcacaatg aaaatcgcga
     1681 ttttaaactg atagaaagca taggatggtt tatagaaacc ggaatggtaa aagtgtattg
     1741 tcccgacagc atagataaac acagctggta taacaaggaa gcttctccgg ccgacagggt
     1801 attgaatcac atgctttacg accgaatgat catggaagaa atcattcccg ggataacaaa
     1861 agactcaggt tatagccgca ttggtgttgc cggatgtagt ttcggtggtt atcatgcctg
     1921 caattttgct ttccgtcacc ctgacttaac ggctgtgatg atttcattgc atgctaaatt
     1981 cgatatcacc ggacagcttg acgggtatta cgatgacaat gtgtattata acaacccgcc
     2041 ggatttcgtt ccgggattga gcgatgagca gttgtggaag atgcagatat ttttaggctc
     2101 tgccgaatat gacatgtgtt tagatgctaa ttaccatatg gcacatttac ttggtacaaa
     2161 gcaggtacca catgtgcttg aagtggagca aggcgataaa catgattggc cttgctggcg
     2221 aaaacaattg cctagatttt tatctttgat acagtacgac aatttacact cttgaattaa
     2281 ccctaaataa tttgcaatga aacgaatagg aattgtacat ggcatggaag attcattccc
     2341 taatgccttt atagaaagaa taaacagtaa acgcatagat ggcattgttg ctgaacgtgt
     2401 gttgatcgac aaggtagtgc aaggtgcagc cagcggttat gatgtgattc ttgatcgcat
     2461 ttcacacgat gcgccatttt atcgtgccta catgaaaaat gctgccatca gtggtgctgc
     2521 tgttatgaat aatccttttt ggtggagtgc tgatgagaaa ttttttaata atgcattagc
     2581 agttaaaatc ggaattcctg ttcctaacac ggtgttattg ccttcgcatg atttacctga
     2641 aaacacaaaa gatacatcct tccggaattt gaaatatccg ttggactggg aatcgatatt
     2701 taattacatc ggatttcctg catatatgaa accacatgac ggtggtggat ggagaggtgt
     2761 ttcgcgttta gaaaatatgg aagagttttt cagtaaatat gctgaaaccg gcgatgatgt
     2821 aatgattttg caaagtgagg ttacttttga ttattacttc cgttgttatt gcatgggcgg
     2881 taaatacgta cacattatgt attacgaacc acgaaatccg catcacctcc gttatacctg
     2941 gaatggccct caggcaccac aaagtttgtt ggataccgtg aaaacctata cgcttgcatt
     3001 gaatcaagca ctgggttatg atttcaatac ggtagaatgg gctgttaaag atggtattcc
     3061 atatgccata gatttttgca atcctgcacc tgatgctgat ttacagagtg taggtgaaga
     3121 aaatttcgaa tgggttgttg aacatgctgc gctgatggca atagaacgcg cattggcaca
     3181 taaaccgggc caggacaatc tgaattgggg aacctatgtt cgcaattctg ttggcggagt
     3241 gccgcattta ccacaaacac aaccgatatt taattcggca ttatcctatt aatttgttga
     3301 cgactgcaca tgagtattta taaagatttc acattaggca ttgaggaaga gtttcagatt
     3361 atcgatcccg aaacacgcga tctatcgcac cgcaaacatc atattattga tgcggccgcg
     3421 aaatataatg tcgatataaa agcagagatg caccagagtg tagttgaagc tgcaactacc
     3481 atctgtaaag acattcacga tgcccgcaat caggtggtaa agttgcgcag gatcattgca
     3541 gatcttgccg acaaggacaa tctgaaaata gcggcagcgg gaacacatcc atttgcagca
     3601 tgggaagaac agttgataac gaaccatccg cgttatcagg agatcgtgaa tgaatttcag
     3661 gaagcagcgc gcagtaatct gatttttgga ttgcatgtgc atattggcat gccatcgcgt
     3721 gatgtggcgg tgtttatgat gaattcgttg cgatacttca ttccacacat ctttgcattg
     3781 tctacaaact cgccattttg ggaaggaaga gataccggat ttaaatcatt ccgcacgaaa
     3841 gtatttgata aatttccgcg caccggtatt cctgatgtgt ttaatgatgc cagccacttc
     3901 gataatttta ttgcattgtt ggtaaaaaca aattgtatcg acaacggcaa aaaaatatgg
     3961 tgggatcttc gtgtgcatcc atactttaat accatcgaat tcaggatgtg cgatattccg
     4021 atgcgcgccg atgaaacaat tgccttagca gctttaatgc aggcacttac cgttaagctc
     4081 tataagttga tgaagagcaa tatcggttgg agaaattacg atcgtgcatt gattcaggaa
     4141 aacaaatggc gtgccgctcg ttacggcatc agtggtaaac tgattgattt cggtaaacag
     4201 gaagaatcag aaacgcgcgg cttagtatta gaactgctcg attttgtgga tgatgtagta
     4261 gatgagttgg gcagtcgcga agcattggaa aatgtgcgtt ggatattaga taatggcacc
     4321 ggtgctgatc gtcaggtgaa gatgtattat gaaaagaacc gcgactttaa agcgcttgcc
     4381 gattatatga tagatgaaac atatattggt atatgatgta atgtaaacag gttaatcaag
     4441 cataataaaa aagccaccgg gaaaccgatg gctttttttg ttgcaatatt ttttttactc
     4501 gattacaatt tcgcgcacta atttctgttg gtcaatagtt gcttcaatca aatatactcc
     4561 cgaaggaatt tgtccggaaa catttaattg gtcgtctatt tcaccattat ctacaattat
     4621 aaatgcctcg tacatcattt gaccgagcat tgaataaatt ttcacggagg cttcgccatt
     4681 gatatctgca ccaaaatcaa tttgaagcgt aaaatctccc tgacttggat ttggataaat
     4741 cacgaatgat gcatcagctt catcctgata aggacttgct tcgtggcagg tttgcacaac
     4801 cgaatatgct gaagatgtac cgaagcatcc tgtaggatca atttgtttca ctttgtaatt
     4861 tccggaggta gtggcattat atttattggt cacttctcca aacaacggca ctgccccttt
     4921 ataccactgg gaagtagacc caattttatt cttgcaactc aatttcaccg gattattcac
     4981 acaaagatca tttgatgccg atgtgagcga cacaacaggc ttaggattat tatttacggt
     5041 gacagtggta attgctgaat aggtgataca acctgcagga gctgtatatt tcactttata
     5101 actgccggat tgggtagcag tgtaagtaat acctgttgca ccggcaattg gagatgtacc
     5161 tttataccat tggcgattaa atccactaat aatcggacta gccgtaaaag taacagaact
     5221 tcccttacag aattcaacag gaccactggc agtaattaca ggatttaccg gatcttcatc
     5281 aagaaaggca tcgcagtcgt tattgattaa atcgcaataa tctgcaacac cgggatgcac
     5341 gagaccgtta aggtcattac aatcactatt attgtctaca taacctaccg gttgattaca
     5401 agcttctaca ctgatcaaag ccgaaccata ggtatcaaaa tctgcatcga gataccaagt
     5461 agggcgacct gttatgagtg gatcagcatc atcaacaagc aaattacagt tgttgtcaat
     5521 tccgtcacaa atttctgaag caagaggatg aataccacta tgaaggtcat tgcaatcaag
     5581 accatttgta acatatccga taggcaaaaa acaatttaaa gtggtcatag tgatgtttcc
     5641 atatccatcg ctgtccatat cggcatagaa caaaatttta cctgttacat cagggtcttc
     5701 atcatcggta agtccatcgc aatcatcatc taataaattg catacttccg gagtgccgtg
     5761 tgtttgcagg gaatcggcat cattacaatc gccaaaaatg gcaacatagt gttcgggagc
     5821 aaagcaattg agactggtat ttgcccaatc gccataaccg tcattatcta tatctgcata
     5881 ccagatagtt tgaccagtga tgtcgggatc tgcgtcatcg actaatccat cgcaatcatc
     5941 atcatacgca ttgcaaattt caggagaacc aatagtaaaa tcagcgttgc tatcattgca
     6001 atcaccaaaa gtaattacat aaccttccgg tgcaccgcaa gcgataatgg tgatcgtatc
     6061 atttccgagg ccgtcgccgt cgttatcagc ataccaaatt gtctgaccgg taatatcagg
     6121 gtcagcgtca tcaaacaacc cgtcgcagtc atcatcaatt ccattacaaa tttccggacc
     6181 tccgatagtg taagcaggat tattatcatt gcaatcgcca ccaacactga tatatccaat
     6241 cggggcaaag caacttacta tttggactgt atcatttcca agaccgtcaa aatcattatc
     6301 gaaataccat gttgtcattc caacaacatc aggatcagca tcatcgataa atccgtcaca
     6361 atcattatct atgccatcgc aaatctctat tgcagccggg tgaatagaag ctgatgtatc
     6421 attacaatca ttcgggttat tgacgtagcc gggtaattca ccaaaaacga tcatactagt
     6481 tgccggatcg ccatagccat cgttatcaat atcttgatat ctgatatagg catccaggtc
     6541 gttatcaatt ataccatcgc agtcattatc aatgtcatcc ggtatttcat aacttccata
     6601 caaaccacaa tctccggatt caaaaggcag tgtatcgaat ttctctaaac gcaactgagc
     6661 aagtatggca cggtattcta aaattttttc gtgatatttc ggattagcca cctgattggt
     6721 atcctgcaaa ggatcagtgg tcagatcata aaattcttct gtccagcaat cgcaattagt
     6781 agttgtatac aaataatcaa aacttctcac acctctgata tcgggaacgt gatgtacatc
     6841 gtcgggttct gttccttcat attcataaaa agcataaggt ctgagtgtat ccggttcagc
     6901 aaggtcgcgc aatgatttcc ccataaatcc aaaggtgtcg ggaataccaa ttgcatcgag
     6961 aattgttttt gggatatcga ttaattcagc aaggtcatta tcaataaatt gacctgccgg
     7021 aaaccatgca ggatagcgca catacaatgg cacacgaata gattcttcaa gaggcatcac
     7081 tttagcgcgc atcatatgtt cgccaattaa atagccattg tcgctcgtaa agaaaataat
     7141 agag
//
LOCUS       DAOQHY010000156         7098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_5569_length_7098_cov_5.268352, whole genome shotgun sequence.
ACCESSION   DAOQHY010000156 DAOQHY010000000
VERSION     DAOQHY010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7098
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5569_length_7098_cov_5.268352"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            330..938
                     /locus_tag="PLL28_11580"
     CDS             330..938
                     /locus_tag="PLL28_11580"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HNF70009.1"
                     /translation="MFKQFNKESEKLRFETFVDAILAIIMTILVLEFKVPEEPFDSDS
                     SLLDHLSHLIPSVLSYVITFSTIVILWLDHHNLFRMLKKADIPLVMLNFLFILFLSAT
                     PFTTSLSGRNFHNPYAIAIMAANYLLMNISFSAIWVYVQQKKLMDENISAQFNNKFEN
                     IIILIGILLQIACIPLAFVNTAIPFMLFVVILILHLIRLLRH"
     gene            complement(1297..2700)
                     /locus_tag="PLL28_11585"
     CDS             complement(1297..2700)
                     /locus_tag="PLL28_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70010.1"
                     /translation="MKKLFFLLAMSTQVLFAQNIGFNGAGLFENYDRDVEQYLADLNQ
                     PFTIRFPGGSISKFHDPYNVRRGWGMSAENIKDWFQRTGFDEDGNGMEKWVRKAEEQP
                     DHSYMDDLIAMQKRFPQMTVLWVLNVLNSTPEANMQAIRYLMNGGVHIVGVEAGNEIY
                     GKYATFDEYIHDFEPIFKMMRAEYPDVKLALIAGANLNRKELVRWNDAMAQYKGDYDA
                     VVLHYYYTSRELGEAYDMIPLRGKYDPEKPNKDLDKAYAKAADLLAKNDLLGNGIKYA
                     KQQFKGKEIWITEWNTKPSDMLNNTILNGAWQFEQLITMRQQAEYLIIHNGVSPDKYG
                     MISKTNKYDSEKGEMLKRIGYFAYMLAGEAGDAQKISPTEKNVISAPVNGVVRMYFTN
                     TECNPFTPNIDLGKYKGADITINYVAGKHLYSGSGTTGYMGKGSVPSYEVKGITREKF
                     SGSIPAYSFGYLEIRVK"
     gene            2832..4466
                     /locus_tag="PLL28_11590"
     CDS             2832..4466
                     /locus_tag="PLL28_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70011.1"
                     /translation="MQAQQPSGNSYVPLGGDTYHLIDRMDIQYPLSYQYLNTSIKPFL
                     RSDVASLTQTWEHSDTLSKRERFQYDYLGNDNREFMNPHSNDYFEGDLFHFFYPEPAT
                     FYQFFSVRINPVLHNEFGVSSDSSNLRFINTRGLELRGSVDDKVGFYLYATDNQAVLP
                     NYIQDRVDAAPQVMPGDGVTKIFKDNGVDYFTTHGYISFNPTKHIEMQFGQDKMFIGD
                     GIRSMIWSDNAKDQLMLRINTHLWRLHYLNVFTELANYDGSNIYNSLIHKKYAAFHYL
                     TVPIIPQIEVGVFESIIFDRYNANGYETGFELNYLNPVIFYRAVESGLGSADNALVGA
                     TLKTKLWNTASLYGQLVLDEFVFGELFGGNKWWGNKYALQAGAKYINAFNIEQLDLQY
                     EFNMARPYTYSYEDDNGSSYTHYAQAIAHPLGANFKEQLFRVWYQPARKLTIRNTTMI
                     AQQGIDSAGINYGSNIFLDYNTYASAYNNITGQGISQTTLLNDLLVSYAFWHNTFLDL
                     HITYRDVNSELVENDNNELYFSIGVRMNEGVRGFMW"
     assembly_gap    4755..4854
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4947..5225)
                     /locus_tag="PLL28_11595"
     CDS             complement(4947..5225)
                     /locus_tag="PLL28_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70012.1"
                     /translation="MRQGKAMSIYFNPLFWIPIIFSIGLIVFGVVLITDNPDNTANLG
                     EVGGNSEAASTVAQSLMQTLQPAFTLFGILIIILGVCIGGRFLYKIGR"
     gene            complement(5332..5985)
                     /locus_tag="PLL28_11600"
     CDS             complement(5332..5985)
                     /locus_tag="PLL28_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70013.1"
                     /translation="MKKHLIFFCCLTLYFQQLSAQTPFETTIPDTAEIKKWIHIKGHI
                     VDELLWTDSLGENLLITTETGDFISPNNPEQVDGEDAELYAYHYLFAIDGAIETWKVY
                     DYIHLCPVDMIAAFVDSTTHITDLDSNGIAEIWLMYRTVCHGDLSPLTMKIIMYESDQ
                     KYALRGDTKIYMGTDDKGVKHYMGGDYKADAAFINGPPVFLQHAISMWEKHLVVYDY"
     gene            complement(6178..7071)
                     /locus_tag="PLL28_11605"
     CDS             complement(6178..7071)
                     /locus_tag="PLL28_11605"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNF70014.1"
                     /translation="MMLQERSIGIKIAEARKKQNLSQAELGRLISISPQAVGKWERGE
                     SMPDITTLNRLAEILGVDLNYFSENFPSIIDQHPTEQLAPLTDSPLIRIKKKLNRNMS
                     AGNWVDADFSGIRDLQEKFSGSNMKNCLFRNADLSGLTLASNNIDKCDFTGTDLRDSS
                     IRSSNLMLNNFSSCSLIDTQFVRNNIGSCNFTETNFSGAEFTSCNFEKNDLSGAVWKF
                     TTFKNCAFSEIIFEGPMADCHFENCSFYHVTFQTALLTNTFFKYNRRFKKVKFIDCKV
                     DSLTYAFLKNNQADLSGVVVV"
BASE COUNT         2016 a         1524 c         1362 g         2096 t
ORIGIN      
        1 tgatacctga tacttgatac ttgatacctg atacttgata cctgatacct gatacctgat
       61 acctgatact tgatacctga tacctgatac ctattacttg atacctgata cttgatacct
      121 gatacttgat acctgataca gactattaat ttcatcgcca caatgaatca accgcgcccc
      181 tccgcttccg cagcgtgaaa aaaacgaacc ataaacaaca caagctcccg tttcaaatgc
      241 aatagctgct catttcaata tgcattagaa tccttaattt tacggtacac attaaaccca
      301 ttcttcggtc aaccccaaca cacctaacta tgtttaaaca gtttaataag gaatcagaaa
      361 aactgcgctt cgaaacattt gttgatgcca ttcttgccat tatcatgacc atactggtcc
      421 tggaatttaa ggtacctgaa gaaccatttg actctgacag cagcctcctt gatcatctat
      481 cacatttaat cccttccgtg ctaagctatg ttatcacctt ttcaactatc gtgattttat
      541 ggctcgacca tcacaattta tttcgtatgc ttaaaaaagc cgacattccg ctggtgatgt
      601 tgaatttctt attcatattg ttcctttccg caactccctt tactacatca cttagcggtc
      661 gtaattttca taatccctat gcaattgcca tcatggctgc aaactatctg ctcatgaata
      721 tttcgttctc agccatatgg gtttatgtgc agcaaaagaa actaatggat gaaaacattt
      781 ccgcacagtt caataacaaa tttgaaaata tcattatcct aatcggcatt ctgcttcaaa
      841 tagcatgcat tccactcgca tttgtgaata ctgcaattcc attcatgtta tttgtagtaa
      901 tacttatttt acatttgatc aggctattga ggcattgaag gagagaggag agaggagaga
      961 ggagaaagga gagaggagag aggagattgg gtggagatgt tatggattgt gtttttttgt
     1021 ggatttgcgc agagaaaatt tatttggctg cgcacaaggt cactgcagta aaccaaccgc
     1081 gttcctccgc ctcgccttgc cctctgcgtg aaataaaaca cataccacaa gcgcgccacc
     1141 ctacctcatt ttatttagaa ctttgagcct ctcggcattg aagaaaattt cattcgatgg
     1201 atatatttcc ttgtaacagc acaaagtcta attacccaac aactacttct tgcagccgag
     1261 taattagact tgaaataatt tatttgaaaa acgtaattac ttcaccctaa tctctaaata
     1321 tccaaagcta taagcaggaa tcgagccgga gaatttttct cgtgtaatgc cttttacttc
     1381 gtaactgggc acactgcctt ttcccatata gcctgtagtt cccgagccgc tatataggtg
     1441 ctttccggca acatagttaa ttgtaatatc agccccttta tattttccga gatcaatatt
     1501 tggcgtaaac ggattacatt ccgtatttgt aaaatacatg cgcacgacac cattaaccgg
     1561 agcactaatc acattctttt ctgttggtga aattttttgc gcatcaccgg cttctcctgc
     1621 aagcatataa gcaaaatagc cgatgcgttt caacatttca cctttctcgg aatcatactt
     1681 attcgtctta ctgatcatgc catatttatc gggactcacc ccattatgaa tgatcagata
     1741 ttcggcttgc tgacgcatgg tgatcaattg ctcgaactgc catgccccgt tcagtatcgt
     1801 attgttgagc atatcggaag gcttggtatt ccattcggta atccatatct ccttaccttt
     1861 gaattgctgc ttcgcatatt taattccatt tcccagtaaa tcatttttcg ccaataagtc
     1921 tgcagccttt gcgtacgcct tatcgaggtc tttattgggt ttctccggat catactttcc
     1981 acgcaaagga atcatatcat aagcttcacc caattcgcga ctggtataat aataatgcaa
     2041 taccaccgcg tcatagtcgc ccttatattg cgccatggca tcgttccagc gcacaagttc
     2101 ttttcgattg agattggcac ctgcaatgag tgctaatttc acatcaggat attcggcacg
     2161 catcattttg aaaataggct caaaatcatg gatatactca tcgaaggtgg catatttacc
     2221 atagatttca ttccctgcct cgacaccaac aatatgcact ccaccattca tcaaatatcg
     2281 tattgcctgc atattcgctt ccggtgtact attaagcacg tttaacaccc atagcactgt
     2341 catctgcgga aatcgcttct gcatggctat cagatcatcc atataactgt gatcgggctg
     2401 ctcttctgct ttgcgcaccc atttttccat gccattgcca tcttcgtcaa aaccggtgcg
     2461 ttgaaaccaa tctttgatat tttctgcact catcccccac cctctgcgga cattgtatgg
     2521 gtcatggaat ttgctgatgg aaccgccggg aaagcgaatg gtgaatggtt gattcaggtc
     2581 ggcaaggtat tgctcaacat cccggtcata gttttcgaaa aggccggcac cgttgaatcc
     2641 aatgttctgt gcgaacagga cctgtgtgct catggcaagt agaaagaata gttttttcat
     2701 cttggggttt tttagtaact ctgcaaaaat taacaatatc cgtgcaaatg tcgtgcctca
     2761 ggtggcattc cgcctgcaaa tgtagaattt tccttttggt aagcctgctg atatgcagtg
     2821 gaaacaaggt aatgcaggcc caacagcctt ccggtaacag ctatgtaccg cttggcggcg
     2881 atacatatca cctgatcgat aggatggata tccaataccc cctctcctac caatatctga
     2941 acacctcgat taaaccattt ttacgcagtg atgtggcatc gctgacccaa acatgggaac
     3001 acagtgatac actttcaaag cgcgagcgat ttcaatacga ctatctcggc aacgataatc
     3061 gcgagttcat gaatccgcat tccaacgatt attttgaagg cgatctgttc catttctttt
     3121 atcccgaacc tgctactttt tatcaatttt tttctgtgcg tataaatccg gtattgcaca
     3181 atgaattcgg tgtttccagc gatagttcaa atctcagatt tatcaatacc cgtggattgg
     3241 aattgcgtgg cagtgtagac gataaggttg gattttattt gtatgccacc gataatcagg
     3301 cagtgcttcc caattatatt caagatcgcg ttgatgccgc accacaagta atgccgggag
     3361 acggtgtgac caaaatattt aaggataatg gggttgatta ttttaccaca cacggctata
     3421 tcagcttcaa ccctactaag catatcgaaa tgcagtttgg acaggataag atgtttattg
     3481 gcgatggcat tcgcagtatg atatggagcg ataatgctaa agaccagctc atgttacgca
     3541 tcaatacgca tctgtggcga ttgcattacc tcaatgtatt taccgaactc gctaattacg
     3601 atgggtcgaa tatctataat agtctcatcc acaaaaaata tgcagctttt cattatctca
     3661 cagtacctat catcccgcaa atagaagttg gtgtatttga gtctatcatt tttgatcgat
     3721 ataatgccaa tggttatgaa accggattcg aattaaatta tcttaacccg gttatcttct
     3781 atcgcgcagt tgaaagcgga ttgggcagtg ccgacaatgc cctggtaggc gcaacgctca
     3841 aaacgaaact gtggaacacc gcctcactat atggacaact tgttctcgac gaattcgttt
     3901 tcggcgaatt atttggcggc aataaatggt ggggaaataa atacgcgtta caagcaggtg
     3961 caaaatatat caacgcattt aatatcgaac aactcgatct gcagtatgaa ttcaatatgg
     4021 cgcgaccata tacctacagc tacgaagacg acaatggctc cagctataca cactatgcgc
     4081 aggcaatagc gcatccgctt ggagcgaatt tcaaggaaca actatttcgc gtctggtatc
     4141 aacctgcccg caaactcacc atacgcaata ccaccatgat tgcgcaacaa ggcattgaca
     4201 gcgccggcat caactacggc agcaatatct tcctcgacta caatacttac gcctctgctt
     4261 acaacaacat caccggacaa ggtatatcac aaaccaccct tctcaatgat cttctggtat
     4321 catacgcttt ctggcataac acctttctcg acctacatat cacctaccgc gacgtaaaca
     4381 gtgagcttgt cgaaaatgac aataatgagc tttactttag tattggggta aggatgaatg
     4441 agggggtgag ggggtttatg tggtaggaga ttaattagct aatttgagaa ttagctgatt
     4501 agcaaattta ggtaattatt tgcatggaga caacagcttt agtttgcaag tttgaaagtt
     4561 ggcaagttag caaattggga attagaaaat ttgagaatta gcaagtggtg gtgcttttaa
     4621 cgctggcgcg ttaatagagt atgtaattat tttttttgca aaattttatt accgaagagt
     4681 aattagctcc ctctctatcg acggtaggag atggagaggg cggggggtga ggtgagagtt
     4741 ggcaagttag caaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaggtaa
     4861 ttatttgcat ggagacaaca gctttagttt gcaagtttga aagttggcaa gttagcaaat
     4921 tggggtgatt gcttgcatgg gttacattat cttccaattt tgtaaagaaa tcttccccct
     4981 atacatacac ccaaaataat aatcaatatt ccgaataagg taaatgcagg ctggagagtc
     5041 tgcatcaatg attgcgctac cgtcgatgcc gcttcgctgt tgccgccaac ctcaccgaga
     5101 ttggctgtat tatccggatt atccgtaatt aatactacac cgaaaacaat aagtcctatc
     5161 gagaaaatga tagggatcca gaataatgga ttaaaataaa tgctcatcgc cttcccttgt
     5221 ctcataaata attaccttaa aactactata gaactaaatt aaaacatcaa cgacacattg
     5281 gcacaggaaa ctttgtatca atttataaaa catttcaaga atttggccgg gctaataatc
     5341 ataaacaacc agatgcttct cccacatgga gatggcatgt tgcagaaata caggtgggcc
     5401 gttgatgaat gcagcatcag ctttgtagtc accacccata tagtgtttta cgcccttgtc
     5461 atctgtgccc atataaattt ttgtatcgcc gcgcagggca tacttttgat ctgattcata
     5521 catgatgatt ttcatagtta acggactcaa atcgccgtga caaacggttc gatacatcaa
     5581 ccatatttca gctatgccgt tgctatcgag gtcggtgata tgtgtggtgc tgtcgacaaa
     5641 cgctgcaatc atatctacgg ggcatagatg gatataatca tatactttcc aggtttcaat
     5701 cgctccatca attgcgaata aataatgata ggcatataat tctgcatctt cgccatcaac
     5761 ttgctccgga ttatttgggc tgataaaatc cccggtttca gttgtaatta atagattctc
     5821 gcctaaactg tcagtccata gcagctcatc cacaatgtga ccttttatat gaatccattt
     5881 tttaatttcg gcagtatcgg gaattgttgt ttcgaaaggt gtttgggcgg ataattgttg
     5941 aaaataaaga gtgaggcaac agaaaaatat caagtgcttt ttcatgctat taaaataaaa
     6001 agcatcagat gatccggaat gcattttgca gggaattcta ttcatgattt gggaacctgg
     6061 ttactaagtt aattgattgg catgaaataa gggcactgca agctgaatta taagctatta
     6121 ccaggccgca gtcaggaaat aaaacgacaa ttcaaccaaa ctattatgca aaatcattca
     6181 taccaccacc accccactta gatcggcctg gttatttttg agaaaggcgt aggtgagtga
     6241 gtcaactttg caatctatga attttacctt cttgaagcgg cgattatact tgaaaaacgt
     6301 atttgtaagc agcgcggtct ggaaagttac gtgataaaac gaacaattct caaagtgaca
     6361 atcagccata ggcccttcga aaataatttc gctgaaggca cagtttttaa aggtggtaaa
     6421 tttccaaacg gctcctgata aatcattctt ctcgaaatta caggaggtaa attccgctcc
     6481 ggagaaattt gtttctgtaa aattgcaact tccgatatta tttctcacaa attgtgtatc
     6541 tatcaaagag caactcgaaa aattattcag catcaaattc gacgagcgaa tgctactgtc
     6601 gcgaagatca gtgcctgtga aatcgcattt gtcgatgtta ttactggcga gtgttaaccc
     6661 ggataggtcg gcatttctga acaggcagtt tttcatatta gacccgctga atttctcttg
     6721 caggtctctg atacctgaga aatcggcatc aacccaattt ccagccgaca tattcctatt
     6781 caatttcttc ttgatcctta tcaagggtga atccgttaaa ggtgcgagct gttctgtagg
     6841 atgttgatca ataatagaag ggaaattttc agagaaatag ttcagatcaa cgccaaggat
     6901 ttctgctaag cgatttagtg tggtgatgtc gggcattgac tctcccctct cccatttccc
     6961 aacggcttgt ggactgatag atatcagtcg tcccaattca gcttgcgata aattctgttt
     7021 ctttctggct tctgcgatct taattccgat cgatctttct tgtagcatca tatgttttgt
     7081 tttgaagtcg caaagcta
//
LOCUS       DAOQHY010000157         7075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_5602_length_7075_cov_5.577066, whole genome shotgun sequence.
ACCESSION   DAOQHY010000157 DAOQHY010000000
VERSION     DAOQHY010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7075
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5602_length_7075_cov_5.577066"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PLL28_11610"
     CDS             complement(<1..204)
                     /locus_tag="PLL28_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase"
                     /protein_id="HNF70015.1"
                     /translation="MHDLAARIANLNESATIKMAQLTRELKGQGRDIVDLSLGEPDFE
                     TPLHIREAAKKAIDDGWTHYTPVA"
     gene            complement(298..1680)
                     /locus_tag="PLL28_11615"
     CDS             complement(298..1680)
                     /locus_tag="PLL28_11615"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNF70016.1"
                     /translation="MTYSEIIHLQRKYYAEGHTLSYAFRRQQLEKFLELVIAHEEAIL
                     DALQKDLHKSSFEGWGVEVGVIITETKYFIRHLKHWMKPKRVKTPLLHFRSRSYIKPV
                     PHGNTLIIAPWNYPFLLAMRPLIGAIAAGNTAIIKPSEHAIHTAKLMEAMINDHFDPA
                     FIRVLLLDASGSAALSAEKFDFIYYTGGANIGRSIYTSAAKNLTPVVLELGGKNPCII
                     DETADIDITASRITWGKFSNCGQTCVAPDYLLVHTSVKDKLVTAIIKNIESFFQNELI
                     NTEDYGRIINDFHVERISRLISNMKIIYGGDVLPNQRYISPTLIEIDDLQSPIMQEEI
                     FGPVLPVLTYNDFSEIKQIIAHNPDPLVLYIFSKNKQHIGICSNEIGSGDMVVNEVIL
                     HFGHLHLPIGGKGSSGFGKTQGKYTFNAFSHQKSLMHRSYFPDLTVRYPPYNSRKSGF
                     IRWVFKYFFS"
     gene            complement(1694..3493)
                     /locus_tag="PLL28_11620"
     CDS             complement(1694..3493)
                     /locus_tag="PLL28_11620"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70017.1"
                     /translation="MRILTLATLSFLFIQTNKAFSQACATCSIDYTYTSPGVYPDTLP
                     PATAGEFYEADITFVMQDDTTVDVVGTLDFLNYYIMEPVGMPYGMHVSTDLGDLPVNY
                     DPTVSLYGCARVCGTPLVAGWYEVTVPLVATLEFPGGDQAASYSIYLEVLPAAPTGGG
                     IIPTASFGCEPLNVDFETGVHSMGADGFSYTWDFGNGASSTSEFPPSQSYVAVGGVPT
                     DYVIMHTVSIDTIGYTLDYVTALSSGCDDCTIFGCTGLIESEKPDLYIIIADLGVNTE
                     PGFANTSPPVTFSLGNTLDPLHTYTMQMKDDDGGLAGSDDNCGSFTFDGNDVGISTLS
                     AGSHQVEVSISHPVITYTFYDTITVYPEVTVPVLDILGETTFCSTDSAVLSTTEVAGL
                     TYQWYKDGDPIPGGDATSLVVYTSGSYFLSATGTGGCYANSDSVVITVFEQPTAPNII
                     VIGNTLTTSSTWDVQWYYNGDPIPGATSTSYTPAIEGLYQVAAVNGPCMAYSVEVDFV
                     FQSVNEVFMPSLQITPNPNEGQFICDFSVTELQDIQIRVTNMLGEVVYSNELHNIIGN
                     NQFAIDLTPADTGIYLLDIQSSKGHIIRKIVVQ"
     gene            3641..4873
                     /locus_tag="PLL28_11625"
     CDS             3641..4873
                     /locus_tag="PLL28_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70018.1"
                     /translation="MKINNLLNAFILCFSILTTVFVLDGCEPPGCTREYNFTGEMPVY
                     MTYEELRASVYLDAPKPIDSSNAFCISDPYLFVSDFEKGVHVYDCTIPENPQDIGFIH
                     VPGVTQLAAVGSRLYVNSYVDLVVLDISDIHAIHETGRVENTLTYRTYGSIFADPSKG
                     VIIGFEERDTVIVEECGYHSSGIGMVYYDEPYLYMPYASGKQSGYLSYYKANGDSLIC
                     FDEGTIRIFDVNNSDNPTVSFTTEVGGTYTVEPIGEYFLIGNSIYTSPASNQLNYLSY
                     LSEADYCSKIVVNQNYCYAANRTTDECYSYGTNLVAINIDYVTSPNYLDYYTDVVPND
                     VFTLNDWVFVIHDHSELRMYHIEADGTLTLFDSIPVENAEYVKASENTVIVDTNPGYK
                     IYTYNGTSGLEYASYIPE"
     gene            complement(4875..>7075)
                     /locus_tag="PLL28_11630"
     CDS             complement(4875..>7075)
                     /locus_tag="PLL28_11630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNF70019.1"
                     /translation="YGGGITFANGYPVTIGAYQETWTPGAGAYVADITVSKFSADGTS
                     LIYSTYLGGSNEDLPYSLIVNEANELIIYGATGSNDFPTTAGAYDASFNGGVLETVDY
                     VLTFTSGSDAFVAKLSADGADLVGSTYIGGTANESLNTGVTSYNYGDHARGEVNLDGA
                     GNIYIAGCTRSTNFPTTAGVMQNSNGGALDGFVAKFNPALTSLSWSTYVGGNGDDGAY
                     AIKYLSSGDVVIVGGTSSNNLNTTPSALHPSYQGGLTDGYVLVMTPDASSIAAMTYIG
                     TSAYDQTYLLDVDELDNIYITGQTLGDYPIVGTVYSNPGSAQYISKLNTNLSTLLLST
                     LLGDGDAQVNISPTAFLVDNCHNIFLTGWGGLVNSGFNPATGSMVSMPITADAFQSST
                     DGSDFYLIIFGNELETMAYATYFGGSISDEHVDGGTSRFDKQGIVYHAVCAGCGGNDD
                     FPTTEGVVSNTNNAFNCNLGVFKFSLGPPPTAAAFLATPDKGCVPLEVFFENESINAD
                     SYEWDFGDGTTGIATDESHVYTEPGTYTIRLVASVDDECGINDTVYQTVEIYEIPIAD
                     FTSSPDIASVYYPVEFTDASIVAVEWDWSFGDGFTSSLQNPTHLYADRGTYTVCLVAT
                     NADGCSDTTCREIIITALSLLDVPNAFSPNGDGTNDVFLPLNYGLTDFLFRVYNRWGE
                     LIYETTDPTKGWNGIYNGVEQEMDVYVYTVSGKGEDNVFYSKQGNFTLVR"
BASE COUNT         2061 a         1466 c         1475 g         2073 t
ORIGIN      
        1 tgctacggga gtatagtgtg tccatccatc atcaattgct tttttcgcag cttcgcgaat
       61 atgcaatggc gtctcaaaat ccggttcgcc taagctcaaa tcaactatat cacgcccctg
      121 accttttaac tcgcgtgtga gttgtgccat tttgatagta gcagattcgt tgagattggc
      181 gattctagcg gctaaatcgt gcatgcaact ggtttaaggt gcgaaattag gaaaaattgg
      241 tggcctcacc tccaaatcag gcttttctgc aatgtgggaa accacgtgtt ttggacacta
      301 actgaagaaa tacttaaaaa cccatctgat aaagccggat ttgcgactgt tatatggcgg
      361 atatcgaact gttaagtcag ggaaataact acgatgcata aggctcttct gatgactgaa
      421 tgcattaaac gtgtattttc cctgtgtttt tccaaagcca ctgcttccct ttcctccaat
      481 aggtaaatgc aaatgtccga agtgcaaaat cacttcattc acaaccatat caccactgcc
      541 tatttcattt gagcaaatac caatatgttg tttgttttta gaaaaaatat acaataccag
      601 tggatccgga ttgtgtgcta tgatctgttt gatctcggaa aaatcattgt aagtgagcac
      661 gggcaataca ggaccaaaaa tttcttcctg catgatgggc gattgcaagt catcgatctc
      721 aataagcgta ggtgaaatat atcgttgatt aggcaataca tctccgccat aaattatttt
      781 catgttactt atgagtctgc tgatccgttc aacatgaaaa tcatttataa tacgaccgta
      841 atcctcagtg ttgatcaatt cattctgaaa gaatgactca atatttttta ttatcgccgt
      901 gacaagttta tccttaaccg atgtatgcac caacaaataa tccggagcaa cgcaggtttg
      961 tccgcaattg ctgaatttac cccatgttat tcgcgaggct gtaatatcaa tatcagcagt
     1021 ttcatcaatg atgcaaggat ttttgccacc aagctctaat acaaccgggg tgagattttt
     1081 tgcagcagag gtgtaaatgg atctgccgat atttgcgcct cctgtataat atatgaagtc
     1141 gaatttctcg gcactcaggg ctgctgagcc ggatgcatca agaagtaata cgcggatgaa
     1201 tgccggatcg aaatgatcat ttatcatagc ctccatcagc tttgctgtgt gtatggcgtg
     1261 ctccgatggt ttgatgatag ctgtatttcc tgcagcaata gcaccaataa gtggacgcat
     1321 ggccaacaga aagggataat tccaaggtgc gatgattaag gtattaccat gcggaaccgg
     1381 cttgatataa ctacgggatc tgaaatgcaa taatggcgtt tttacacgct ttggcttcat
     1441 ccaatgttta agatgtcgga tgaaatattt tgtttcagtg ataattacac ctacttcaac
     1501 gccccagcct tcgaatgacg atttatgcag gtctttttgc aatgcatcca ggattgcctc
     1561 ttcatgcgca ataactaatt caagaaactt ttcgagttgt tgccttcgaa atgcgtatga
     1621 caaagtatga ccctctgcat aatatttgcg ctgcagatga attatttcag aataggtcat
     1681 agaaatacct cgtttattga acaacaatct tgcgtatgat atgtcccttg ctactttgta
     1741 tgtcgagtaa ataaattcct gtatctgcag gagtgaggtc tatagcgaat tgattattgc
     1801 cgattatatt atgcaattca ttggaataaa ccacttcacc caacatattg gtaacccgaa
     1861 tttgaatatc ttgtaattca gttacactga agtcgcaaat gaactgtcct tcgtttggat
     1921 ttggtgtgat ctgtaaactt ggcataaata cttcattaac agattgaaac acaaaatcca
     1981 cttcaacaga atatgccata catgggccat ttacagcagc tacctgatac aaaccttcaa
     2041 tggcaggagt atagctggtg gatgttgcgc ccggaattgg atcgccattg taataccact
     2101 gaacgtccca agtagaggac gttgtaagtg tatttccgat gactatgatg tttggggcag
     2161 ttggttgttc aaacacggtg attaccactg aatcagaatt ggcatagcaa ccgcctgttc
     2221 ctgtagcact caggaaatag cttccggagg tatataccac aagtgaagtt gcgtctcctc
     2281 ccggtatagg atcaccatcc ttataccatt ggtaggttaa acctgcaact tccgtggttg
     2341 aaagtactgc actatcagta gagcaaaatg ttgtttcacc aaggatatca agtacaggaa
     2401 ctgtaacctc agggtatacg gtaatggtat cgtaaaatgt ataagtaatc acggggtggg
     2461 agatgcttac ttccacctga tgacttcctg cgctcaatgt gcttatgcct acgtcatttc
     2521 catcgaatgt aaatgagccg caattgtcgt cactacctgc caaaccgcca tcatcatcct
     2581 tcatttgcat ggtataggtg tgcagtgggt cgagtgtatt tccgaggctg aaggtaaccg
     2641 gcggtgaagt gttggcgaaa cccggttcgg tatttacgcc aagatcggca ataatgatgt
     2701 ataagtcagg tttctcggat tcaatcagtc ccgtgcatcc aaagattgtg caatcatcac
     2761 atccactgga aagtgcggtc acataatcta gggtatatcc tattgtatca atagaaaccg
     2821 tatgcatgat cacataatca gtgggaactc ctccaacggc tacataactt tgagaaggcg
     2881 ggaattctga ggtgctggat gcgccattgc cgaaatccca ggtataactg aatccatcgg
     2941 cacccatact atgcacgccg gtttcaaaat cgacattcag tggttcacag ccaaatgaag
     3001 ctgttggtat aatgcctcca cccgttggcg cagcgggaag tacttccaga tagatgctat
     3061 atgaagctgc ctgatcgcct cccggaaatt caagcgtggc aaccagtgga acggtaactt
     3121 cataccatcc tgctacaaga ggcgtgccac aaactctggc gcatccataa agtgatacgg
     3181 tagggtcgta attaacaggg agatcgccta aatcggttga aacatgcatc ccataaggca
     3241 ttcccaccgg ttccataatg tagtaattca gaaaatccag ggtgccgacc acgtctaccg
     3301 tagtgtcatc ctgcatcacg aaggtgatat ctgcttcata aaattcgccg gcagttgcgg
     3361 gtggcaatgt gtcgggatat acccccggtg aagtataggt gtagtctata ctacaggtgg
     3421 cgcaagcttg actgaaggcc ttattagtct gaataaaaag aaatgacagt gtggcaaggg
     3481 taagaatgcg catgtatgca attttgggtt gaagttagtg gaattttggt ttgcgtgaaa
     3541 tgatgtgggc ttgaagtagg accggctcag ggaaatactt tagacgagtt acgtcattca
     3601 tgatattcat ggtatttggt atttttgtta aaatccggtt atgaaaatta ataatctcct
     3661 caatgccttt atattgtgct tttccatttt aacaactgtt tttgtgttag atggatgcga
     3721 gcctcccggc tgtaccagag aatacaactt cacaggtgaa atgcctgttt atatgactta
     3781 tgaggaatta agggcttctg tttatttaga tgcacctaag cctattgata gttcaaatgc
     3841 attttgtatc agtgatccat atctgtttgt cagcgatttc gaaaagggtg tgcatgtgta
     3901 tgattgtact attcctgaga atccacaaga catcggattt attcatgttc ccggtgtaac
     3961 gcagttggca gctgtgggaa gcagactcta cgtaaacagt tatgtcgatc ttgtagtatt
     4021 agatatttca gatatacatg ctattcatga aacgggtagg gtggaaaata cacttacgta
     4081 tagaacgtat ggttcaatat ttgcagatcc atcaaaagga gtaattatcg ggtttgaaga
     4141 aagagatacc gttattgttg aggaatgcgg ttatcatagc tccggaattg gcatggtata
     4201 ctatgatgaa ccatatttat atatgccata tgcaagcggt aaacaaagcg gatatctctc
     4261 gtactataaa gcaaacggcg attcgttgat ttgctttgat gaaggtacca ttcgcatttt
     4321 tgatgtcaat aattccgata acccaacggt atcgtttaca acggaagtgg gtggcactta
     4381 tactgttgaa cctattggag aatattttct tataggaaat tctatttata catctcccgc
     4441 aagtaatcag ttaaactacc tttcgtatct ttctgaagct gattattgca gcaaaatagt
     4501 cgttaaccaa aattattgtt atgcggcaaa caggactacg gatgaatgct atagttatgg
     4561 gactaattta gtggcaataa atatcgatta cgtaacatcg ccaaattatt tagattatta
     4621 taccgatgtt gtacctaacg atgtttttac attaaacgat tgggttttcg tgatccatga
     4681 tcacagcgaa ttaaggatgt accatataga agctgatgga accctcacct tatttgattc
     4741 aattcctgtt gaaaatgcag aatatgtaaa agcatcagaa aacaccgtta ttgtagatac
     4801 aaacccgggt tacaaaattt atacctacaa cggcacttca ggattggaat atgcatccta
     4861 tattccggaa taatttatct tacaagtgta aaattccctt gcttggagta aaagacgttg
     4921 tcctctcctt tacctgaaac ggtatacaca taaacatcca tttcctgttc tacgccattg
     4981 tagatgccat tccatccttt tgtaggatct gtggtttcgt aaattaattc tccccagcga
     5041 ttgtaaacgc gaaatagaaa atcggtaagt ccataattta atgggaggaa aacatcattc
     5101 gtgccatctc catttggaga aaatgcatta ggaacatcca gtaaacttaa tgcagtaatt
     5161 ataatctcgc gacaagttgt atctgaacat ccgtcggcat ttgttgctac aaggcataca
     5221 gtgtatgttc cgcggtcggc atacaggtga gtcgggttct gtaaagagga ggtaaatcca
     5281 tcaccaaagc tccaatccca ttcaacagct actatgctgg catctgtaaa ttctaccggg
     5341 tagtaaacag atgcgatatc cggactcgat gtaaaatctg caatagggat ttcataaatt
     5401 tctactgttt gataaacggt atcgtttata ccacactcat catctactga agcaacaagt
     5461 ctaatagtgt atgttcccgg ttccgtataa acatgcgatt cgtctgttgc aatccccgta
     5521 gtaccatcgc caaaatccca ttcataacta tcggcattta tactttcgtt ttcaaaaaac
     5581 acttccaggg gaacgcagcc cttgtcaggc gttgctaaaa atgcagctgc agttggcggc
     5641 ggacctaaag agaatttgaa tactcctaaa ttgcaattaa atgcattatt ggtatttgaa
     5701 acaacgcctt cagttgtagg aaaatcatca ttcccaccac atcctgcaca aacagcatga
     5761 taaacaattc cttgcttatc gaacctactt gttccgccat ctacgtgttc atcagaaata
     5821 ctgccgccaa aataggtagc atatgccatc gtttccagtt catttccgaa aataattaaa
     5881 taaaaatcgc ttccgtcggt tgatgactga aatgcatctg ctgtaatagg catgctcacc
     5941 atacttccag ttgcaggatt aaatccggag tttaccaatc ctccccaacc ggtcagaaaa
     6001 atattatggc aattgtcaac caaaaatgca gtaggtgaaa tatttacctg agcatctccg
     6061 tcacccaaca aggtactcaa taataaggtg ctgagatttg tatttaattt cgaaatatat
     6121 tgtgcacttc cgggattgct gtacacagtt cctacaatag gataatcgcc aagtgtttgt
     6181 ccagtgatgt agatattatc cagttcatca acatcaagta aatacgtttg atcgtaggcg
     6241 ctggttccga tataggtcat ggctgcgatg ctcgaggcgt caggtgtcat caccaacaca
     6301 tagccatcgg ttaagcctcc ttgataggat ggatgcaagg ccgaaggtgt agtatttaaa
     6361 ttattgctgc ttgtgccgcc aacaattact acatcgccgg aacttaagta ttttatggca
     6421 taagcaccat catcgccatt accgccaaca tatgtcgacc atgataatga agtcaatgcg
     6481 ggattaaatt ttgcaacaaa tccatctaat gcaccgccgt tggaattttg cattacacct
     6541 gctgtagtcg gaaagttagt tgatctggtg catcctgcaa tatagatgtt tccggcccca
     6601 tcaagattta cttcaccacg tgcatggtca ccataattgt aacttgttac gcctgtattc
     6661 agcgactcat tagcagtgcc tccgatgtag gtcgaaccaa ctaaatcagc gccatcggca
     6721 cttaatttag caacaaatgc atcgctgcct gacgtaaatg taagcacata atctactgtt
     6781 tcaagcacgc caccattgaa cgaagcatca taggcgcctg cggtggttgg gaaatcatta
     6841 gatcctgttg caccataaat gattagttca ttagcttcat ttacaatcaa tgaatagggt
     6901 agatcttcat tagaaccacc gagataggta gaatatatta atgaagtgcc gtcagcagaa
     6961 aattttgaaa cagtgatgtc tgcaacatac gcgccggctc cgggggtcca tgtttcttga
     7021 taggctccta tagtaaccgg atatccattg gcaaaggtaa ttcctccgcc ataca
//
LOCUS       DAOQHY010000158         7061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_5621_length_7061_cov_6.638310, whole genome shotgun sequence.
ACCESSION   DAOQHY010000158 DAOQHY010000000
VERSION     DAOQHY010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5621_length_7061_cov_6.638310"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="PLL28_11635"
     CDS             <1..549
                     /locus_tag="PLL28_11635"
                     /inference="COORDINATES: protein motif:HMM:NF017102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HNF70020.1"
                     /translation="ALVFFQLNEKYTWRPFITKGKKIDSFNGVYVYFNGDVSHVEGRN
                     LSPDGYNFGLKFQCVEFVKRYYYDALHFKMPDTYGNAVDFFNPVVSDGKLNNARGLLQ
                     YSNGSNSKPAIDDILVFDVSSSNPYGHVAIISDVTEQGIEIIQQNPGRFVKSRVNYDL
                     IHRGNKWIIDNKKVLGWLRVPN"
     gene            621..1100
                     /locus_tag="PLL28_11640"
     CDS             621..1100
                     /locus_tag="PLL28_11640"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNF70021.1"
                     /translation="MAEQHGFWHKYRSTIVYGISLALLMFLLKWLELRFIIYDHSFEI
                     YVGFIAFIFTGLGIWLALKLSKPKVETIIIEKQVIINADVDFTPNAALISQLDISSRE
                     MDVLNLMAKGHSNAEIARELFISLSTVKTHTGNLFVKLDVKRRTQAIEKAKRLQIIP"
     gene            1208..1756
                     /locus_tag="PLL28_11645"
     CDS             1208..1756
                     /locus_tag="PLL28_11645"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HNF70022.1"
                     /translation="MKKNILTYGLISGTIVSAIMLFSMNYFSHCEGTVDYTTSMLIGY
                     ASMLLSFSLVFVGVKNYRDKFNGGFITFGKAFQIAILMVLIASTMYVISWVIDYFFFM
                     PDFMDKYAETMIQNLKDSGASAAEVEAKTKEMAVFAERYKNPLFTAMMTYMEILPIGI
                     VVALITSLILRKKPSQPAIDTM"
     gene            1974..3407
                     /locus_tag="PLL28_11650"
     CDS             1974..3407
                     /locus_tag="PLL28_11650"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNF70023.1"
                     /translation="MKRSSTTMFMLICSISLLAGQPNQQVLDYIEQYKAIAVQEMIEY
                     KIPASITMAQGILESGAGNSELAKESNNHFGIKCHTDWTGDKVYYDDDEKGECFRKYD
                     EVAQSYRDHSIFLSTKPRYASLFELDADDYKGWAKGLKAAGYATNPKYADLLINLIEE
                     YQLQELDKMTMADIKKSNKKDDHKKDDVKKDDVKKDDKNIAKNDEQQDVKEEKTTKKF
                     TWGGYDADVYYFNRIPTVTVKQGETPESLAQKHHVKISLLRDYNDIEDGKPLDVGEKC
                     YLQPKRKKGHDKYHTVVAGETMWTISRDEGVRLAYLYKYNDIEQGQEPAVGEEVYLRF
                     KRHDVMKLATVTNKQEVKSTPEKIIPKPEKEKSAVEQSPVKETYQDTNNDIYIDFEEE
                     KPAPVEAKPEIKTTSTQEINSPKTITAEPVQQDAILVEQYHIVQPKETLYAISKLYGV
                     TVEQIQQWNKLSDNNIRIGQQLIVGYK"
     gene            3404..3931
                     /locus_tag="PLL28_11655"
     CDS             3404..3931
                     /locus_tag="PLL28_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNF70024.1"
                     /translation="MITLHNLQFEPFIDQAEIKSRIAEMGHSLSNKYIGKEPVFVIVM
                     KGAFMFAADLLQQFHGNCTLHFTKVQSYVGTKAGEITQFSGLPEGLEGKDVVIIEDII
                     DSGNTVAFLLEQLKHVPLNSLFTIALLQKDIPRNTFQSADLIGFIIPDKFVVGYGLDY
                     NELGRNLPAIYQAIT"
     gene            complement(3938..4687)
                     /locus_tag="PLL28_11660"
     CDS             complement(3938..4687)
                     /locus_tag="PLL28_11660"
                     /inference="COORDINATES: protein motif:HMM:NF040120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HNF70025.1"
                     /translation="MPIFDPRERSGPILPALILANVIPLVGIIYYDVSFFALFYLYWW
                     ETVIISIFRWQKMGWASKPSEPDPNVTYNGKMLTYEHVNNRRGMRIQYFFIRSGMMLF
                     YLLFIVVFVGIMTAVQEDATSFAMALTFTDKWVFWSFIGFVALHLIEYITWIQTEQYK
                     ETTIAELASPFDARVLVMHVVIVGGTFLAMFTSQKLFPDHPHAASIGYGTLFVFLKTI
                     IDVIAWNNNHSRQKAVVKVGNALRGKSNSSK"
     gene            complement(4692..5114)
                     /locus_tag="PLL28_11665"
     CDS             complement(4692..5114)
                     /locus_tag="PLL28_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70026.1"
                     /translation="MKLFNFILGAFLVFITLTFMRCNEVTEITELVKNTEKIQIVFYN
                     GDHQDTYIDITDKSEIKNFGDFVDNKSTPILNCKYDGKIVFFLETNTPSGQKNSTSME
                     FSLSEDCKQVGYMYNQMWQTKHLTNEGYKYLLNLASKQ"
     gene            complement(5141..5431)
                     /locus_tag="PLL28_11670"
     CDS             complement(5141..5431)
                     /locus_tag="PLL28_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase subunit beta"
                     /protein_id="HNF70027.1"
                     /translation="MQAAESIKDILSKNKPYLVSKYSLSSIGVFGAVLQDDFSGGMAV
                     EIIVDFKKPIGIEIVDLGDELETLIHHKVDLVTRNGIEKSYYNLFRKEIQYI"
     gene            5508..6107
                     /locus_tag="PLL28_11675"
     CDS             5508..6107
                     /locus_tag="PLL28_11675"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNF70028.1"
                     /translation="MINLILFGPPGSGKGTQAEHLKEHFQLLHISTGDLLRNEIANGT
                     KLGMEAKQFMDAGQLVPDEVVIGMLGGAVDQSRSMGKRGIIFDGFPRTTAQALALEHL
                     LADKGTAVSVVLSLVVEEEELTQRILKRGLTSGRSDDNDIDTIRRRVQEYRTKTEPVA
                     GFYKDRDLLVEIEGVGAIEQITQALMNAVNAYSSKHSDG"
     gene            6229..>7061
                     /gene="obgE"
                     /locus_tag="PLL28_11680"
     CDS             6229..>7061
                     /gene="obgE"
                     /locus_tag="PLL28_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNF70029.1"
                     /translation="MENKNFVDYVKIHCKSGKGGAGSVHFHRDKHTIKGGPDGGDGGR
                     GGHIILRGNAQLWTLLHLKYRKHVIAGDGAYGVGQLRKGADGEDIVLEVPLGTVAKDF
                     ETDEVELEIVEDGQEVIWVPGGRGGLGNNHFKSPTRQTPQFAQPGEPAIEAWKILELK
                     VLADVGLVGFPNAGKSTLLSVVSAAKPEIANYPFTTLVPNLGMVAYRDHRSFIMADIP
                     GIIEGASEGRGLGIRFLKHIERNSLLLFMISCDEKNIADTYKILVNELKKYNKELLTK
                     KR"
BASE COUNT         2173 a         1449 c         1505 g         1934 t
ORIGIN      
        1 gcacttgtgt tttttcaatt aaacgaaaaa tatacatggc gaccatttat tactaaaggc
       61 aaaaagattg atagcttcaa tggcgtttat gtttatttta atggagatgt ttcacatgtt
      121 gaaggaagaa atcttagccc tgatggctat aattttgggt tgaaatttca atgtgtcgag
      181 tttgttaaac gatattatta cgatgcacta cattttaaaa tgcccgacac ttatggcaat
      241 gcggttgatt ttttcaaccc tgtcgtgagc gacggaaaac tgaataacgc ccgtggatta
      301 ttgcaatatt ccaatggcag caacagtaaa ccggccatag atgatattct tgttttcgac
      361 gtgtcttcat caaatcctta tggtcacgtc gctattatat cggatgttac tgaacagggc
      421 atcgagatca tacagcaaaa cccgggcagg tttgtcaaat cacgggtgaa ttatgacttg
      481 attcatcgag gtaataagtg gattatcgac aataaaaaag tgctgggttg gctgcgagtt
      541 cctaactgaa tgccttattc ggtcgatccg cttacttccg cttgatcgcc actcttctcc
      601 aaatttcaga tatttgcaat atggcagagc agcatggttt ttggcacaag taccgttcta
      661 ccattgtata tggtatcagc ctggctttgc tcatgttcct gcttaagtgg ctcgagctgc
      721 gtttcataat ttatgatcac agctttgaaa tttatgtcgg atttattgcc ttcattttca
      781 cgggattggg tatctggctg gcactcaaac tgtcgaaacc caaggtggaa accattatta
      841 tagaaaagca ggtgattatt aatgccgatg tcgattttac acccaatgct gcacttatca
      901 gccaattgga tatcagctca agagaaatgg atgtgctcaa tctgatggct aagggacaca
      961 gcaatgccga gattgcacgc gaattgttta tttccctcag cacagttaag acacataccg
     1021 gaaacttgtt tgtcaaactg gatgtcaagc gccgaacaca ggctattgag aaggcaaaac
     1081 gacttcaaat catcccataa ttaccatact ttagtaggaa tcggcactat ttcaggcaaa
     1141 aatggccgaa agtatgaccg gaaaaatacc ctgcaacgcc acatttgcgg cataaaaata
     1201 aactcaaatg aaaaagaaca tccttaccta cggcctcatc tcaggaacca tcgtttctgc
     1261 gatcatgctg ttttctatga attatttcag tcactgtgaa ggcactgtcg actataccac
     1321 cagcatgctc atcggctatg cctcgatgtt gctatcattt tctttggtat ttgttggtgt
     1381 aaagaactat cgcgataaat tcaacggcgg attcatcacc ttcggcaaag ccttccaaat
     1441 tgctattctt atggtgctta ttgcctctac catgtatgtg atttcatggg tgattgatta
     1501 cttcttcttc atgcccgact ttatggataa atacgccgaa acgatgattc aaaatctcaa
     1561 agatagtggc gccagtgctg cagaggtcga ggccaaaaca aaagaaatgg ccgtatttgc
     1621 ggagcgatat aaaaacccat tgttcactgc tatgatgacc tatatggaga tactgcctat
     1681 cggaattgtt gttgctttaa tcacttctct gatactccgt aaaaaaccga gtcagcctgc
     1741 aatagacacc atgtaatctt gtttttatcg gctaaaagcg cgaggtatgc atgcagtgtc
     1801 ggcactgttt atgcatttct tcgcgccttt tttatgctca ttgtatgttc tcaaaattgg
     1861 attctgccct tcattttagt ggaaaacaca tgcaaggcac ggttttgggg tgagataatt
     1921 ttacattggt gcattacgtt tggtaaaggc gcctttgaaa acctatgtcg agtatgaaaa
     1981 gaagcagcac aaccatgttc atgctaatct gctccatcag cctgttggcc ggtcagccca
     2041 atcaacaggt gttggactat atagagcagt acaaggccat tgccgtgcag gaaatgattg
     2101 agtataaaat tcctgccagt atcaccatgg ctcaaggtat cctggaaagc ggcgcaggaa
     2161 atagtgaact cgcaaaagaa tcaaataacc attttggtat taaatgtcat accgactgga
     2221 ccggcgacaa agtgtattac gatgacgatg aaaaaggcga gtgctttcgc aaatatgatg
     2281 aagtggcgca atcgtaccgc gatcatagta tttttctcag caccaaaccg cgatatgctt
     2341 ccttgtttga attagatgct gatgattata agggttgggc gaaaggattg aaagctgccg
     2401 gttatgccac taatcctaaa tatgccgatt tgctcattaa cctcattgag gaatatcaat
     2461 tgcaggagtt ggataaaatg acaatggctg atatcaaaaa atcaaacaaa aaggatgatc
     2521 acaaaaagga cgatgttaaa aaagatgatg taaagaaaga tgataaaaac attgcaaaga
     2581 atgatgagca acaggacgtg aaggaggaga aaacaaccaa gaagtttacc tggggcggat
     2641 atgatgctga tgtatattat tttaatcgca taccaaccgt taccgttaaa caaggtgaaa
     2701 caccggaatc actcgcccaa aaacatcatg taaaaatcag cctgctgcgc gattataacg
     2761 atatcgaaga tggtaaacca ctcgacgttg gcgagaagtg ctatctgcaa ccaaaacgaa
     2821 aaaaaggtca tgataaatat cataccgttg tagcaggaga aaccatgtgg acgatttccc
     2881 gcgatgaagg tgtgcgattg gcatatttat ataaatataa tgatatcgag cagggacaag
     2941 aacctgcagt tggcgaggaa gtatatctgc gatttaaacg acatgatgtc atgaagttgg
     3001 caacggttac aaataagcag gaagtgaaat caacacccga aaaaataatt cctaaaccgg
     3061 aaaaagaaaa gtctgctgtt gaacaatcgc ctgtaaaaga aacctatcaa gacaccaaca
     3121 atgatattta tatcgatttt gaagaggaaa aaccggcgcc ggtggaagca aaaccggaaa
     3181 taaaaaccac ctcaacgcaa gaaatcaatt cgccaaaaac aataactgct gaacctgttc
     3241 aacaagatgc cattttagtt gagcaatatc atatcgtgca accaaaagaa acactttatg
     3301 ctatttccaa attatatggc gtaacggtag aacagattca gcaatggaat aaattgagtg
     3361 ataataatat ccgcatcggt caacaattaa ttgtcggata taaatgataa ccctccacaa
     3421 cctgcaattc gaacccttca ttgatcaggc ggaaataaaa tcgcggattg cggaaatggg
     3481 gcattccctt tcaaataaat acattggaaa agaacctgta tttgtaattg taatgaaggg
     3541 tgcttttatg tttgccgccg atttattgca acaatttcat ggaaactgta ccttgcattt
     3601 caccaaagtg caatcttatg tcggcactaa agccggagaa ataacgcaat tctcaggtct
     3661 tcctgaaggc ctggagggaa aagatgtggt gattattgaa gatattatcg actccggaaa
     3721 taccgttgca tttttattag aacagcttaa gcatgttcct ctcaattcct tatttaccat
     3781 tgcacttttg cagaaagata ttccgcgcaa cacatttcaa tccgccgacc ttatcggatt
     3841 tatcattccc gataaatttg tggtaggcta tggattagat tataacgagt tgggtagaaa
     3901 tttgcctgca atttatcagg caataaccta agcaatgtta ctttgaggaa tttgattttc
     3961 ctcgcaaggc gttgcctact ttcacaacag ctttttgtcg tgaatgattg ttgttccagg
     4021 caataacatc gatgatcgtt ttcaaaaaaa cgaacaaagt gccataacca atactcgctg
     4081 catgcggatg atcgggaaat aatttctgac ttgtaaacat ggccaggaat gttccgccaa
     4141 caattaccac atgcataact aaaaccctgg catcaaaagg tgaagctaat tctgctatag
     4201 ttgtttcttt gtattgttcg gtttgtatcc aggtgatgta ttctatcagg tgtaatgcaa
     4261 caaacccaat gaaactccag aacacccatt tgtctgtaaa ggtcagcgcc attgcaaatg
     4321 aagttgcatc ttcctgaacg gcagtcataa tgccgacaaa cacaacaata aacagtaaat
     4381 aaaataacat cattccggag cgaatgaaga aatattgtat ccgcattcct cttcgattat
     4441 tcacatgctc ataggtgagc attttaccat tgtaggtaac attaggatcg ggctcggaag
     4501 gtttggaagc ccatcccatt ttctgccagc gaaatatgct gatgattacc gtttcccacc
     4561 aatacaaata aaataaagcg aaaaaactta catcataata gattatccca accaaaggaa
     4621 taacattggc aagtatcaat gccggaagta tcggaccgct gcgctctctg ggatcaaata
     4681 tgggcatgat gctattgttt tgatgccaga tttagaagat acttatagcc ctcgttggtc
     4741 agatgttttg tctgccacat ttgattgtac atataaccaa cctgtttgca atcttccgat
     4801 aaactgaatt ccatactggt ggaatttttc tgtccggaag gtgtattcgt ttccaggaag
     4861 aaaacaatct taccatcgta cttgcaattc agaattggcg ttgatttatt atcaacaaaa
     4921 tcgccgaaat tttttatttc tgacttatca gtgatatcta tataagtatc ctgatgatcg
     4981 ccattgtaga atacgatttg aatcttttca gtgtttttca ctaattccgt aatttcggtc
     5041 acttcattgc aacgcatgaa tgtgagtgta ataaacacca aaaatgcacc taaaatgaaa
     5101 ttgaagagtt tcatcagtga ttaaaaatac atcatcttgt tcagatgtat tgtatttcct
     5161 ttctgaacaa gttgtaatac gacttttcaa tgccgttacg cgtaactaaa tccaccttat
     5221 gatgaattaa tgtttcaagc tcatcgccaa ggtcaacaat ctctatacca atgggtttct
     5281 tgaaatcgac gatgatttca acggccatac cacccgaaaa atcatcttgt aacacagctc
     5341 caaacacgcc aatgctgctc aaactgtatt ttgataccag atatggttta ttttttgaaa
     5401 ggatgtcttt tatcgattca gctgcctgca tagctcaaag gtaatcattc cgccacacgg
     5461 aattacctat aacaaccgta tctttgccgc ctaagttcac accgactatg atcaatctca
     5521 tcttattcgg ccctccggga agcggtaaag gcacacaggc agagcatttg aaggaacatt
     5581 ttcaactcct ccatatttct accggcgacc tgctgcgcaa tgagattgcc aatggcacta
     5641 agctgggtat ggaagccaaa caatttatgg atgccggcca attagttccg gacgaagtgg
     5701 tgataggcat gcttggcggc gcagttgacc aatccaggag catgggaaaa cgaggcatca
     5761 ttttcgacgg atttccccgc acaaccgcac aggcactggc tttggagcat ttgcttgccg
     5821 acaaaggaac ggcagttagt gtggtgctta gcctggtggt tgaggaagag gaacttacgc
     5881 aacgcatcct caaacgtgga cttaccagtg gtcgctccga tgataatgac atcgatacca
     5941 tacgccgtcg cgtgcaggaa tatcgcacaa aaacagagcc ggttgcaggt ttctataagg
     6001 atcgcgattt gcttgttgaa atcgaaggcg taggcgctat cgaacaaatc acgcaagcat
     6061 tgatgaatgc cgttaatgct tattcatcca aacattccga cggttaatgt atccgaaagc
     6121 agatttgctg atggctgcta atggatagcc gaagggtgaa tctagcagga tataccacat
     6181 tacttttcgc ccaaagcctt ttaggtcaag ggttttgcaa taaattacat ggaaaataaa
     6241 aactttgtcg attacgtaaa gatccactgc aaaagcggga aaggcggcgc cggttcagtg
     6301 cacttccacc gcgataagca cacgattaag ggtggtcccg atggcggcga tggcggtaga
     6361 ggcggacata tcattttacg cggcaatgcc caattgtgga ccctattgca cctgaaatac
     6421 cgcaaacacg tgattgccgg cgacggggct tacggcgttg gacagttgcg caagggtgcc
     6481 gatggagagg atattgtgct ggaagtacca cttggaacag tagccaaaga ttttgaaacg
     6541 gatgaagttg agctggagat tgtagaagat ggtcaggaag taatttgggt gcccggaggc
     6601 agaggtggac tcggcaacaa ccattttaag tcgccaacgc gacaaacccc tcaatttgca
     6661 caacccggtg aaccggccat agaagcatgg aaaatccttg agctgaaagt gcttgccgat
     6721 gtgggtttgg taggatttcc caatgccgga aaatctacct tgctgagcgt tgtcagtgcc
     6781 gcaaaacctg aaatcgccaa ttatccattt acaaccctcg tgccaaatct cggaatggtg
     6841 gcctatcgtg atcacagaag cttcatcatg gccgatattc ccggaatcat agaaggtgcc
     6901 agtgagggta ggggattggg aattcgtttc ctcaaacata tagaacgcaa ttcgctgctg
     6961 ctgtttatga tcagttgcga tgaaaaaaat attgcggata cttataagat tcttgtcaac
     7021 gaactgaaaa aatataacaa ggaactactc accaaaaaac g
//
LOCUS       DAOQHY010000159         7033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_5641_length_7033_cov_5.427916, whole genome shotgun sequence.
ACCESSION   DAOQHY010000159 DAOQHY010000000
VERSION     DAOQHY010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7033
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5641_length_7033_cov_5.427916"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..1842
                     /locus_tag="PLL28_11685"
     CDS             19..1842
                     /locus_tag="PLL28_11685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNF70030.1"
                     /translation="MKTRILCVAASTFLFYSIGNGQACDFYEDYSDPTPWNFVYVYPG
                     AGGCGDNPQPGTLAVTGGQVNYTDVNDANDTRIWRDIGTPVSDESWTATFDFTPIAIG
                     MDGVPRVGHVIWSLSSGTNCPFNDTWEHCIPNDQDGIMVWYLSEYTPADPTTGFYIYA
                     KNELTYVNTAVGDNITALPGQTYYLTFTRESSNYVSLEVYLDPARTDLLDAIECFEIP
                     DNITNLHILQHGNAPWGYYKRVLTGTLDNTCIINNATTSALLTGPTNVCIDDNPIYEI
                     TGSGTATVSWLPMAGVDVTLIDPNSISIDDWGGATSATITAVISGACSEDTLSIDVTI
                     ANSTIVDTTVTICSSDTVSIFGLEITDAGFYNQTIATAAGCDSIINITVIESDTIYNN
                     EIIEICQGDSIEISGSFISENTVISETLTSVGGCDSVHVITVTIIDAPIVSFPDDTVL
                     CTAIGIVLNPIIADATATISWSPATDLSCANCVAPFLVTTENGWYYITITNALGCSYT
                     DSVYVKVDLSDWGIPNAFSPNGDGINDYFGPIIPLGCVVENFVIYNRWGAEIFRSTGA
                     LNYWDGTYQGIPQEIGAYVYILYANCADYEIQTHGSVTLLR"
     gene            complement(1959..3404)
                     /locus_tag="PLL28_11690"
     CDS             complement(1959..3404)
                     /locus_tag="PLL28_11690"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HNF70031.1"
                     /translation="MKHIVKSAFFITVGGALPLLASVVLLIPYTDNLHTADYGALAIY
                     ISFALLVQILMNYGVDSYLSVHYYDHHEDPKALKSFLAGITGGLLVYGIALTLVLSLV
                     SWLLFPLIFPDGSISFWPYGFMSILTGFFNAWFRMYVNIQVFADKARKYFWFGLFNFI
                     VTIAISAFLVYKYPFSLVGPMWGRLLSGVLIFLLTFGFGLKEFGIRLVYKTGRLLREY
                     STPIVIFSLLTWVLGYINNYILNAFSTTADVGVYDFALKCTLVIEYAGLGLTGAINPR
                     IFQLWKKNGNTESTQEENRYYHAFSAVNVIIISFNILIIPIIIQAFISNESYYESIKY
                     IPLLCVAFTFKGIYSMYVNPVFYFKKTTILPLVLSITALVQIISGAILINFFGIRGAV
                     WSFFLVRPIQIVILWVAVRRIYNFKFNKFKLVVAPILFSTMVIALYYFVQLPFQINGA
                     IQFGLAILIIGVVYRKELLTIPNLVFNKNQK"
     gene            3485..3907
                     /locus_tag="PLL28_11695"
     CDS             3485..3907
                     /locus_tag="PLL28_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70032.1"
                     /translation="MRPIILLIVCLSIMSASCKKGDKVIAFGPDKVYYVSPVEEETAL
                     KVGTFLQQYKYFVGNGAVVQLVEDSVFTARFATHEGIEKDAYIIDGFIDMQLEMSVSI
                     FDGAPVNIQLCDSTLTTKGSISFEDARAWIEGKAYVEK"
     gene            complement(4361..4699)
                     /gene="ssb"
                     /locus_tag="PLL28_11700"
     CDS             complement(4361..4699)
                     /gene="ssb"
                     /locus_tag="PLL28_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNF70033.1"
                     /translation="MNTLKNKVLLMGRLGQDPEVKTVNGKSKLAKFSLATSETYTNAK
                     GEKVKDTQWHNLIAWGKTAEIIEKYCGKGMEVVVEGKLINHNYTDKQDIKRYVTEIQV
                     NEVVIITPKK"
     gene            4909..5265
                     /locus_tag="PLL28_11705"
     CDS             4909..5265
                     /locus_tag="PLL28_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70034.1"
                     /translation="MVIKKCRECGKDILGRNDKKFCSDICRHSHNNRQRAVNGGNYMN
                     TINTILRKNRNILMEISTEENPKVSKNRLYDKGFNFNYITSTYTTKKGAIYYYCYDYG
                     YLPVEGDYVFLVKRKE"
     gene            complement(5450..6385)
                     /locus_tag="PLL28_11710"
     CDS             complement(5450..6385)
                     /locus_tag="PLL28_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70035.1"
                     /translation="MKAGKTIFALLLTCQVGVAFSAEVKTIFTWSYDDKLISMVYYFD
                     QGDYNYYKGQKRVYDNFSHYLDEAPGHEVMKDFANEFKLLASEHNWSDWQLAECAINF
                     VQTLKYRNDGTWEYPRYPIETLVEKGGDCEDLAMLLESLLAELGFDCVLISPERHMGV
                     GIATKYPIQGAAFDHKGKNYYYIETTSAGWGIGDYPENLSANALIYDPGNAYDGLALS
                     EYVYTYHNYVTPNEELATAAQTTTDADNAEVEEAQSPDDQPSNAPVYNPNAPRRPANT
                     YTIDVDKVVIDGKKETVVTKVVEQDGQQTIVADSE"
     gene            complement(6566..>7033)
                     /locus_tag="PLL28_11715"
     CDS             complement(6566..>7033)
                     /locus_tag="PLL28_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF70036.1"
                     /translation="YNHPDKECVCGSQVVQRYLNRISGPLLDRIDLHVEVVPVGVSEM
                     SQMQEGEKSASIRERVIAARKIQEERFAGVEIYCNAQMGSKQVKEVCVISPVGQTLLN
                     KAMEKLGLSARAYDRILKVSRTIADLSASPDIMPEHLAEAIHYRSLDRENWAG"
BASE COUNT         2155 a         1475 c         1321 g         2082 t
ORIGIN      
        1 cgtaaatttg aactaccaat gaaaactaga attctctgtg tcgctgctag caccttcctt
       61 ttttattcca ttggaaacgg gcaggcttgt gacttttatg aagattattc agacccaacg
      121 ccttggaact ttgtatatgt atatccggga gccggtggct gtggagataa ccctcagccc
      181 ggaactcttg cagtaactgg cggtcaggtt aactataccg atgtaaacga tgccaatgac
      241 acaagaatat ggcgagatat cggcacgcct gttagtgacg agtcatggac cgctactttt
      301 gatttcaccc ctattgcgat tggaatggat ggcgttcctc gtgttggaca tgtgatatgg
      361 tcattatcgt ccgggactaa ctgccccttc aatgacacat gggaacactg cattccaaac
      421 gaccaagatg gcataatggt atggtattta tctgaatata ccccggctga cccaacaacc
      481 ggcttctaca tttatgcaaa aaacgaactc acctatgtaa ataccgcggt tggcgacaat
      541 atcacagcat tacccggcca aacatattat ctcacattta ccagagaatc aagcaattat
      601 gtaagtctag aagtgtatct cgaccctgca agaactgatc ttttggatgc gattgaatgt
      661 tttgagattc ctgataacat aactaacctc catattcttc aacatggaaa tgcgccatgg
      721 ggctattaca aaagggtttt gacaggtaca cttgataata cctgtattat taataacgct
      781 actaccagtg ccttgctaac cggccctaca aatgtctgta ttgatgataa tccaatatat
      841 gaaataacag gaagcggtac tgccactgtt tcatggctac ctatggccgg tgtggatgtt
      901 accttgatag atccaaactc catatcaatt gatgactggg gaggcgcaac cagtgctacc
      961 attacagcag tcatatcagg tgcatgttca gaggatacct tatcgattga cgttacaatt
     1021 gcaaactcaa ctattgttga caccacagtt actatatgta gttcagatac tgtttcaatt
     1081 ttcggtttag aaattactga tgccggattt tataatcaaa ccattgcaac tgctgcaggt
     1141 tgtgattcaa ttattaatat tacggttatt gaatcagaca ccatttacaa taatgaaatt
     1201 attgaaattt gtcaagggga cagtattgag atttcaggat cctttatttc tgaaaacaca
     1261 gtaatatcag aaacacttac ctctgtcggc ggctgcgatt ctgtgcatgt tataaccgtt
     1321 actattatcg atgctccaat cgtatcgttc cccgatgaca ctgtcttatg cacagcaata
     1381 ggtattgttt taaatccaat aatagctgat gctacagcta caatttcgtg gagcccagct
     1441 acagatttaa gttgtgcaaa ctgcgtcgcg ccatttttag ttactacaga gaatggttgg
     1501 tattatataa cgattacaaa tgctttagga tgtagctaca ctgacagtgt ttatgtaaaa
     1561 gttgatcttt cagattgggg tataccaaat gcattttctc caaatggaga tggaataaac
     1621 gactatttcg gtccaataat tccgctaggg tgcgtagtgg agaattttgt tatttataac
     1681 cgttgggggg ccgaaatttt tagatccacc ggggctttaa attactggga tggcacatat
     1741 caagggattc ctcaggaaat aggtgcctac gtatatatct tgtatgcaaa ttgtgctgat
     1801 tatgaaattc agacacatgg ctctgttaca ttactaagat agcttttgtt caaagcttat
     1861 accaggtatt taattataac cgtgcaaata ccacattatc cagtgggcta attaataatc
     1921 aattaaatgg cctcctgatg ctacaaatgc caacaaattt atttctgatt cttattaaat
     1981 actaaattag gaattgttaa caattctttt cgatatacta ctcctattat caatatagcg
     2041 agtccaaatt gaattgcacc gtttatttga aacggcaact gaacaaaata atatagcgca
     2101 atcaccatgg tggaaaataa aataggagct acaacgagtt taaatttatt aaatttaaaa
     2161 ttgtaaatcc ttctgactgc cacccacaaa attacgattt gaataggcct cacaagaaaa
     2221 aaactccaaa ccgctccccg tattccaaaa aaattgatca agattgctcc gcttattatt
     2281 tgcactaagg cagtaattga taacactaac ggcaaaattg ttgttttttt gaaatagaat
     2341 acgggattaa catacatgct ataaattcct ttaaaagtaa aggctacaca taataaagga
     2401 atatatttta tactttcata gtatgattca ttgcttataa atgcttgaat aataatcgga
     2461 attatcagta tgttaaatga aataattatg acatttactg cgctaaatgc atgataatac
     2521 ctattctcct cctgcgtgct ttctgtatta ccattcttct tccacaattg aaatattctg
     2581 gggttaatgg ctccagtcaa tcctaagcct gcgtattcaa ttacgagtgt gcacttcaaa
     2641 gcaaaatcat atacgcctac atcggcagta gtggaaaatg catttaatat ataattatta
     2701 atataaccta acacccaggt cagcaagctg aatattacaa ttggagtgct atactctctg
     2761 agcaatctgc cggttttgta aaccagtctg ataccaaatt ccttaagtcc aaaaccaaag
     2821 gtcaataaaa aaatcaggac gccggataat aatcggcccc acattggacc cactaatgaa
     2881 aatgggtatt tatataccag gaaggcggaa attgcaatgg taacaatgaa gttaaacagc
     2941 ccgaaccaaa aatatttacg agccttatca gcaaacacct gtatattcac atacatccta
     3001 aaccaggcat taaaaaaacc ggtgagtatg ctcataaagc catagggcca gaacgaaatc
     3061 gagccatcgg ggaaaatcaa gggaaatagc aaccagctta caagggataa tactagggtg
     3121 agtgcaatgc cataaaccaa taatcctccg gtgatgcctg caagaaaact ttttagggcc
     3181 tttggatctt catgatgatc gtaatagtga accgaaagat agctgtcgac cccataattc
     3241 atgagaatct gaaccagcaa ggcaaatgag atgtaaattg ccaatgcgcc ataatcggcg
     3301 gtatgcaaat tgtcggtata cggaatcagc aatacaacac ttgccaaaag cggcagggct
     3361 cctcctacgg taatgaaaaa tgcggatttg acaatatgct tcacaagtat tcgttatgac
     3421 aaaaataggg atatccaccc gcattgccat tttataaggc gcaagcttac taactttata
     3481 tcttatgaga ccaattatcc tacttatcgt ttgtttatct attatgagtg cttcgtgtaa
     3541 aaaaggcgac aaagtaatag cattcggtcc tgacaaggtg tattacgtgt cgccggttga
     3601 agaagagact gcattaaaag tggggacctt tcttcagcag tataagtatt ttgtaggtaa
     3661 tggggctgtt gtgcaattgg tggaggatag tgttttcact gcacgtttcg ccacacatga
     3721 aggcatagaa aaagatgcct atatcattga cggctttata gacatgcaac tagaaatgag
     3781 tgtttccatt tttgacggcg cacccgtaaa catacagcta tgtgattcta ccctcaccac
     3841 aaaaggcagt attagttttg aagatgcgcg cgcctggatt gaaggcaaag cttatgtaga
     3901 gaagtaatcg ccgctgaatt ttctcaaggc taccatgagc tattaatagc aacagaaatt
     3961 gaccataaaa ttttcattgc ccatttccaa cattattacc tcaacaatcc agtgcaggaa
     4021 aaagaaaact gcctgagaga ttcgcctcct aaaatgacca gcaacaccaa cggcggaatc
     4081 gctacgaggc agtttcttta attggcattc aatacacaag cgaacacctt gcttcgaaag
     4141 gataatcaag caaaatattt ccatgacaac acgacatagc cacgtatgtt taaccgctta
     4201 ttgtcaatct attaaaagct ttgataggat tttaattgac aataattgca aggtgtttcg
     4261 cttgtgaaat atataaaaac tgcctctccc caacctccaa acacgaccag caacaaacaa
     4321 ccggggaatg tgaggcagtt ttatccgagg gaaggatgtt ttatttttta ggtgtgatga
     4381 tcacaacctc gttcacctga atctccgtta catagcgctt gatgtcctgc ttgtcggtat
     4441 agttatgatt gatgagcttt ccttcaacaa ccacttccat tccttttccg caatactttt
     4501 caatgatctc ggcggtttta ccccatgcaa tcagattgtg ccactgtgtg tctttcactt
     4561 tttcaccttt ggcattggtg taagtttcgg aggtagcaag gctgaatttg gcgagtttag
     4621 atttgccatt taccgttttt acttcggggt cttgaccaag acgccccatt aacagcactt
     4681 tgtttttaag tgtgttcata caatttgttt ttaaggttta caatcaaatc cgttttaccg
     4741 gaaccggtgt tcatcaccga cgaagcaaat atcagcattg cccattgtgt ccaccaaatt
     4801 ttaagcgatt atactcgcat ataatcgctt aagtccgata agaatatatt tttatatata
     4861 tttgtcatgt tatatcctaa tccttaaaaa tgaaattctt gtttatctat ggttataaaa
     4921 aaatgcagag agtgcggcaa ggacatcctt ggacgcaacg acaaaaaatt ctgtagtgat
     4981 atctgcagac actctcacaa caatcgtcag cgcgccgtaa atggtggcaa ttacatgaac
     5041 accatcaata ctattttgcg aaaaaatcgc aacatattga tggaaatctc tacagaggaa
     5101 aatccgaaag tcagcaaaaa ccgattgtac gacaaagggt ttaacttcaa ttatatcacc
     5161 agcacctata ccacaaaaaa aggtgctata tattattatt gctacgatta cggttatctc
     5221 cctgtagaag gtgattatgt ttttctggta aaacggaaag agtagaacct cagcattaca
     5281 tctgaaggta ggcaccgaaa acattgagca attaaattgc acatgcgctt tacaatttgt
     5341 gaagttagct ttctatttcc cataatttca caaaaaatga ctgacacata cacatcgcct
     5401 ggtggcgagg ttaaaccatt gtgctagaat ttactgctga aaaatgtggc tattcactgt
     5461 cggcaactat tgtttgctga ccatcttgct ctacgacttt agtaaccaca gtttcctttt
     5521 taccatcaat tacaaccttg tctacatcga tggtataagt gtttgccggt cgacgcggag
     5581 catttggatt atataccgga gcatttgatg gttgatcatc gggcgactga gcttcctcaa
     5641 cttcagcatt atcagcatct gttgtagttt gggctgcggt agctaattcc tcatttgggg
     5701 taacataatt gtgataggta tacacatatt cagataatgc caaaccatca taagcattac
     5761 ccggatcata aataagggca ttggcactta aattttcagg ataatcacct attccccacc
     5821 ctgcacttgt agtttcgatg tagtaataat ttttcccttt atggtcaaat gctgctccct
     5881 gaatcggata ctttgttgca ataccaactc ccatatggcg ctcaggtgat atgagcacac
     5941 aatcaaatcc caattctgca agaagagatt ccagcaacat ggccaggtct tcacaatctc
     6001 cgcccttctc aactaatgtt tcgattggat accgagggta ttcccaggtg ccatcatttc
     6061 tatattttag ggtctggaca aaattgatag cacattcggc caattgccaa tcgctccaat
     6121 tatgttcaga agccaataat ttaaattcat tggcaaaatc tttcatcacc tcgtgccccg
     6181 gagcttcgtc cagataatga ctaaaattgt catatacccg cttttgccct ttgtaataat
     6241 tgtaatctcc ctgatcaaaa taataaacca ttgagatcaa tttatcgtcg tatgaccatg
     6301 tgaaaatggt tttcacttca gcgctaaagg caacgccgac ctgacatgta agtagcaaag
     6361 cgaatatggt ttttccggcc ttcatacggt tcttaaccga agttaagcaa taaacttgcc
     6421 agattaagat tttagtatgt gtggtgatgt ggattatgct atcatcatct atgaggccgt
     6481 aatctcagat ttaggactac aagcaccaat agcacttcag aaaacgacgc accaaatcca
     6541 ctaaaccact tgagcgcatt gccttctagc ccgcccaatt ttctctatcc aaactgcggt
     6601 aatgtattgc ttcagccagg tgttcaggca tgatatccgg cgaagccgat aagtcggcaa
     6661 tagttctgct gactttcaaa atccggtcat aagccctcgc gcttaagcca agtttttcca
     6721 tcgccttgtt taaaagtgtt tgtcctacag ggctgatcac gcaaacttcc ttcacctgtt
     6781 tggagcccat ttgggcattg caatatattt ccacaccggc aaatctttct tcctgaattt
     6841 ttcttgcggc aattactctt tcgcgtattg atgcactttt ttcaccttcc tgcatctgag
     6901 acatttcgga gactccaaca ggcactactt ccacatgcag atcgatacga tcgagcaaag
     6961 gacctgaaat tctattcaga tatcgctgta ccacttgtga gccacaaacg cattccttat
     7021 cagggtgatt ata
//
LOCUS       DAOQHY010000160         7011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_5677_length_7011_cov_5.458453, whole genome shotgun sequence.
ACCESSION   DAOQHY010000160 DAOQHY010000000
VERSION     DAOQHY010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 7011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7011
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5677_length_7011_cov_5.458453"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(215..1051)
                     /gene="tsf"
                     /locus_tag="PLL28_11720"
     CDS             complement(215..1051)
                     /gene="tsf"
                     /locus_tag="PLL28_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNF70037.1"
                     /translation="MSVTITASDVNKLRQITGAGMMDCKKALSETGGDFDAAIDYLRK
                     KGQKVSEKRADRETNEGVVVATVSSDSAYGTIFGLGCETDFVAKNEDYVNFAKAISAI
                     AMANKPESKEALLALPFESITIAEKIAEQVGKIGEKIEITDYALITGEHVVAYNHSNG
                     KVGVLVAMNKKGDAIEAVGKDVTMQIAAMAPISIDKNDVPEEVKNREMEIGREQARAE
                     GKPEAILDKIAAGKLEAYYKDATLLNQKFVKDNAKSIRDVLTGVEKDLTVTAFKRVAL
                     GA"
     gene            complement(1139..2026)
                     /gene="rpsB"
                     /locus_tag="PLL28_11725"
     CDS             complement(1139..2026)
                     /gene="rpsB"
                     /locus_tag="PLL28_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNF70038.1"
                     /translation="MEKITYNDLLEAGVHFGHLRKKWNPKMLPYIFMEKKGIHIIDLN
                     KTLEKLDEAAAALKTIARSGKKIMFVATKKQAKEIMEEQARRVGMPFATERWLGGMLT
                     NFTTIRKSIKKLQAIDKMLVDGSAENMTKKERLVLGRERDKLEKVIGGVAQLNRLPAA
                     LLIVDIGHEHIAIAEAHRLNITTFGMVDTNCDPNKVDFAVPANDDAAKSIAIIVKYLT
                     DAIVEGLEERAKSKEEMDEAAREPEFDENGEEIKVNINEEEGRDGRKEGGNRFGKGPR
                     GGGGNKPGGNRRPMAQRKK"
     gene            complement(2045..2431)
                     /gene="rpsI"
                     /locus_tag="PLL28_11730"
     CDS             complement(2045..2431)
                     /gene="rpsI"
                     /locus_tag="PLL28_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNF70039.1"
                     /translation="MDVINTVGRRKNAIARVFMKKGKGSIIINGKDYKEYISVSHLLH
                     NITDPLETVGMSSEFDFTINASGGGVKGQAEAIKMGIARALVEFNAELKPALRAKGFM
                     TRDSRMVERKKFGLRKARRATQFSKR"
     gene            complement(2437..2877)
                     /gene="rplM"
                     /locus_tag="PLL28_11735"
     CDS             complement(2437..2877)
                     /gene="rplM"
                     /locus_tag="PLL28_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNF70040.1"
                     /translation="METLSYKTISANPATVQHDWWVIDAENQTLGRLSTKVAAILRGK
                     NKPYFTPHTDCGDFVIIINAEKVRLTGKKMLEKEIDWYSGYPGGRKVANPKTVLANQP
                     HKLLERSIKGMLPHNKLGSAMYKKLFVYAGSEHPHAAQKPKTLK"
     gene            complement(3081..4409)
                     /locus_tag="PLL28_11740"
     CDS             complement(3081..4409)
                     /locus_tag="PLL28_11740"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNF70041.1"
                     /translation="MRKLEKPPNINDPELMFEMLAFFRDPASQRLVSQLNNKYIYWDK
                     VRYQPVQEGFTQAHLWAAIKMSRTIEAKVVRFGRKKFFYNLTDTIQRGLHEFDMHMGG
                     NLGSRGLIPEEDKKRYLISSIMEEAIASSQIEGAVTSRKVARQMLLDESKPRNKSEQM
                     ILNNFITIKHIVDIKNEPITIERLLHIHQLISNKTLDNESDEGRFRDHDDIHVVDVTD
                     GEIVFTPPAHGEVEEMMRALIDFFNEDDHTTFIHPIIKGCIIHFIMGFIHPFVDGNGR
                     TARALFYWYLLKKGYWLTEYLSISRLIIRSKQQYAMSFLYCETDENDLTYFLNYKIKM
                     MQLAYAHLQDYIKKQIHEKKQLVDFQRIGGVNNRQAQILLWLYVDSNEIFKVNTIENR
                     FGVSNQTARTDLYGLVELGFMEELTINKKERAFIRSASFLDRMGENSDVL"
     gene            complement(4581..5258)
                     /locus_tag="PLL28_11745"
     CDS             complement(4581..5258)
                     /locus_tag="PLL28_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNF70042.1"
                     /translation="MIGAYFIGILFMVAGLIIGGVLRSKFKKYSEIPVNGGLTGKDIA
                     ERMLAENGIYDVQVISVGGQLTDHYNPMNKTINLSEGVYNVCSIAAAAVAAHETGHAV
                     QHARQYSWLQMRSKMVPVVSISSQWLTWVLLAGILLINSYPQILLAGIVMFALTTLFS
                     FITLPVEIDASRRAIAWLQGSSVAGTMEMSKAKDALNWAGYTYVVAALGSLATLLYYV
                     MIYMSRR"
     gene            complement(5390..5956)
                     /locus_tag="PLL28_11750"
     CDS             complement(5390..5956)
                     /locus_tag="PLL28_11750"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNF70043.1"
                     /translation="MLVPVLIIIGVLALILIGLYNTLVSRKNGVENAFGTIDAMLKKR
                     FDLLPNLVETVKQYMTYEQDTLTRIVELRNSVQSNPGMPVQDRMDAENKLTGLLTQLR
                     VAVENYPDLKANNSFIQLQSTWTEIEEQLSAARRTYNAAVTAYNNSVEMFPTNIMAGM
                     MNYRRKEVLAIPESERQSPSAKDLFNKN"
     gene            complement(6081..>7011)
                     /gene="dusB"
                     /locus_tag="PLL28_11755"
     CDS             complement(6081..>7011)
                     /gene="dusB"
                     /locus_tag="PLL28_11755"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNF70044.1"
                     /translation="EDVSDPPFRVLCKEQGADLMYTEFISTEGLIRHAEKSTKKLEFY
                     EAERPIGIQIFGADIDVMKGCAQIVEEAGPDLLDINFGCPVKKVVCKDAGAGILKDIP
                     RMVRLTAEIVKATKLPVTVKTRLGWDDNSKNIMEVAERLQDIGIKALTIHGRTRAQMY
                     KGSADWTLIGAVKNNPRIQIPIFGNGDVDTPEKALEMKNTYGVDGIMIGRAAIGHPWV
                     FREIKYFLNTGEHLAAPTMEERVEVCKKHLLKSVEWKGEVLGILEMRRHYTNYYKGIP
                     NIKSYRNELVNLMDVGALMEVMDTIAQLPEEVM"
BASE COUNT         1827 a         1573 c         1416 g         2195 t
ORIGIN      
        1 gcagcgaata gactgcaagc ggtaagcaac cccagtacaa gaaggattgt gtgtgtgcct
       61 ctcatgattg gtcgcctgtg gaagtataaa gttacgatta ttaaatagat gaaacgccac
      121 caataaaggt tgtatgcatc ttttgtatga tgttttatac aaaaaaggct gccggttgag
      181 gggcagcctt tctgaatttg tacgtaatcg aatattatgc gcctaaagcc acgcgtttga
      241 atgcagtaac tgtgagatct ttctcaacac ctgttaatac atcgcggata gatttagcgt
      301 tgtctttcac aaatttctgg ttaagaagtg tagcatcttt gtaatatgct tccaattttc
      361 cggcagcaat cttgtcgagg atggcttccg gttttccttc ggcacgagct tgttcgcgac
      421 cgatttccat ttcacgattt ttcacctctt caggaacatc gtttttatcg atagagatag
      481 gagccatagc tgcaatctgc atagtaacat ctttaccaac tgcttcgatt gcatcgcctt
      541 ttttattcat ggcaacgagt acaccaactt taccattgct gtgattataa gcaacaacgt
      601 gttcgccggt aatcaaagcg taatcagtga tttcaatttt ttcgccaatt tttccaactt
      661 gttcagcgat tttttcagcg atggtaatgc tttcgaaagg caaagcgagt aatgcttctt
      721 tgctttcagg tttattagcc attgcgattg cggaaattgc ttttgcaaaa ttcacatagt
      781 cttcattttt tgcaacgaag tccgtttcgc aacccaaacc aaaaatagtt ccataagcgc
      841 tatcgcttga aacagtggca actacaacac cttcattcgt ttcacgatcg gcgcgttttt
      901 cacttacttt ctgacctttt ttgcggaggt agtcgatagc ggcatcgaaa tcgccaccgg
      961 tttcgctcag tgctttttta caatccatca ttccggcgcc ggtaatctga cgcagtttgt
     1021 ttacatcact tgcagttatt gttactgaca tattattgag tgttttttat ttaaaaaacc
     1081 cttccgcctg agggaggaag ggttttgttt aatgtgatat taaaacccta acgggcgatt
     1141 atttcttgcg ttgagccatt gggcgacggt ttcctcccgg tttgttacca ccgccaccgc
     1201 gaggtccttt accgaaacgg tttccacctt ctttgcgacc atcacgacct tcttcttcgt
     1261 tgatatttac ttttatctct tcgccattct catcaaattc cggttcgcgt gcagcctcat
     1321 ccatttcttc tttgctctta gcgcgttctt cgagaccttc aacaatagca tctgtaagat
     1381 acttcacgat gatagcgata gatttcgctg cgtcgtcgtt tgcaggaact gcgaagtcaa
     1441 ctttattcgg atcgcagttt gtatctacca ttccgaatgt agtgatattg aggcgatgcg
     1501 cttctgcgat tgcaatatgt tcgtgaccga tatcaacgat caacaatgct gcagggagac
     1561 gattcaactg agcaacacca ccgattactt tttcaagttt atcgcgttcg cgaccgagta
     1621 cgaggcgttc ttttttggtc atgttctctg cagaaccgtc aaccaacatt ttatcgatag
     1681 cctgtaattt tttgatgctt ttgcgaatag tggtgaagtt ggtaagcata ccaccaagcc
     1741 aacgttcggt agcgaaaggc atacctacgc gacgagcttg ttcttccatg atttctttcg
     1801 cttgcttttt tgtagcaacg aacataatct ttttaccact gcgcgcgata gttttcaatg
     1861 cagcagcagc ttcatcgagt ttttcgagcg ttttattgag gtcgatgatg tgaatacctt
     1921 ttttctccat gaagatataa ggcaacatct tcgggttcca tttcttacgc aggtggccaa
     1981 agtgtacgcc tgcttccaga agatcgttat atgtaatttt ttccatctta ctgtacttga
     2041 tcgattaacg tttagagaat tgcgttgcac ggcgtgcttt gcgaagaccg aatttcttgc
     2101 gttcaaccat acggctgtcg cgtgtcatga atcctttagc acggagtgcc ggtttcaatt
     2161 cagcgttgaa ttcaacgagt gcgcgggcga tacccatttt gatagcttcc gcctgacctt
     2221 taacaccacc gcctgaagca ttgatggtaa agtcgaattc tgagctcatg ccaactgttt
     2281 ccagtggatc ggtgatatta tgcaaaaggt gggaaacaga gatatactct ttgtagtctt
     2341 taccattgat gataatgcta ccttttcctt tcttcataaa tacgcgagca atggcgtttt
     2401 tccttctgcc aacggtatta ataacgtcca tgtagattat tttaacgttt taggtttttg
     2461 tgcagcatgc gggtgttcag aacctgcata cacgaaaagt ttcttataca tggcagaacc
     2521 caatttatta tggggcaaca tgcctttgat agagcgttcg agcagtttgt gtggctgatt
     2581 tgccaataca gttttaggat tggcaacctt gcgtccaccc ggataaccgg aataccagtc
     2641 gatttctttc tctaacatct ttttaccggt aaggcgcacc ttttcggcat tgatgataat
     2701 tacgaaatcc ccacaatctg tatgaggggt gaaatacggc ttgtttttgc cacgcaaaat
     2761 ggcagccacc ttggtgctca gacggcccag ggtttggttt tcggcatcga tcacccacca
     2821 gtcgtgctga acggtggcag gattcgccga gatcgtttta tagcttaacg tttccacttg
     2881 ttttgtttta aaaattgcta tcccggtctt aaacgggacg gcaaagatag atttacccct
     2941 cagaaaaacc aaggaatatc aacgaatttt cctacacccc tttcgtaagt aattgatttt
     3001 cagcgaaatt ttggcgtact aatttaatta agtcacctta tttcatgcag tttagtttgc
     3061 atgaaaaagt agtaatcaaa ctacaataca tccgaatttt cgcccattct gtcgaggaag
     3121 cttgcagatc ttataaatgc acgttccttt ttattgattg tcagctcttc cataaagccc
     3181 aattctacta ggccatacag atctgttctc gcggtttgat tagacacgcc aaatctgttt
     3241 tcaatggtat taaccttaaa aatttcattg gaatcgacat acagccacag caatatttga
     3301 gcctgccggt tgttcacacc gccaatgcgt tgaaaatcga ccagttgttt cttttcatgg
     3361 atctgcttct taatataatc ctgaagatgt gcataggcta attgcatcat tttgatttta
     3421 taattcagga aataagtaag atcattttca tccgtctcac aatacaaaaa cgacatggca
     3481 tattgctgtt tactcctgat aatgagcctg gaaattgaca aatactcagt tagccaatat
     3541 cctttcttta acaaatacca ataaaacaaa gctctagcgg tgcgcccatt tccgtcgaca
     3601 aatggatgaa tgaatcccat tatgaaatgt atgatacatc ctttaatgat aggatgaata
     3661 aaagttgtat gatcatcttc attaaagaaa tcgattaatg cgcgcatcat ttcttccacc
     3721 tcaccatgag caggaggcgt gaatacaatt tcgccatcgg taacatccac tacatgtata
     3781 tcatcatgat ctctaaaccg cccttcatca ctttcattgt caagggtttt gttggaaatt
     3841 aattgatgta tatgcagcag tcgctctatt gtaatgggct cgtttttgat atcaacgatg
     3901 tgctttatag taataaaatt gttgagtatc atttgctccg acttattccg aggcttgctt
     3961 tcatccagca gcatttgccg tgctactttt ctggatgtaa cagcaccttc tatctgactg
     4021 gatgcaattg cttcctccat aatagaactt atcagatatc tttttttgtc ctcctccgga
     4081 atcaaacccc gactccccag gttcccaccc atatgcatgt caaattcatg taaacccctt
     4141 tggatggtat ccgtgagatt atagaaaaac ttctttctgc caaaacgaac aacttttgct
     4201 tctatcgttc tcgacatttt gatggcagcc cataaatgcg cttgcgtgaa accttcctga
     4261 acaggttgat accgcacctt gtcccagtag atatatttat tgttcaactg gcttaccaac
     4321 cgctgtgaag ccggatccct gaaaaacgcc aacatttcaa acattaattc aggatcatta
     4381 atgttaggcg gcttttctaa ttttctcata ttatttccta atttatcaaa tatttggaca
     4441 aattacgaaa ttagcaactt gtataaatat ttccaaattt tacacaaatt aggaaatatt
     4501 ggtaaatcga gttggaagtg taataaaaaa ggagaggcaa aacctctcct tatcaatatt
     4561 ttaaagcttt tggccgccga ttaccttctg ctcatgtaga tcatgacata gtacaacagg
     4621 gtagccaatg aacccagggc agctacaacg taagtgtaac cggcccagtt cagggcatct
     4681 tttgctttcg acatctccat ggttccggca actgaagagc cttgtaacca ggcaattgcc
     4741 ctgcgactgg catcaatttc taccggaagg gtgataaagc tgaacaaggt tgtaagcgca
     4801 aacattacga ttcctgctaa taggatttgc gggtatgagt tgatgagcag gatacctgcc
     4861 aacaacaccc atgtaagcca ttgcgatgaa atactcacca caggcaccat tttactgcgc
     4921 atttgcaacc atgagtactg acgggcatgt tgaacagcat gtccggtttc atgggccgca
     4981 acagctgcag cagctatact acacacattg taaacgcctt cgctgaggtt gatggtttta
     5041 ttcattggat tgtagtgatc ggtaagttgc ccgccaacgc tgattacctg aacatcataa
     5101 atgccatttt cagcaagcat acgctcggcg atgtccttgc cggtgagacc gccatttaca
     5161 gggatctctg aatatttttt gaacttactc ctcaatactc cgccgataat caaaccggca
     5221 accatgaata aaatacctat gaaatatgct cctatcattt ggtttgtttt ttattgttta
     5281 ctgacgtttg taacgcaaag atattgcagt ttgttcagtt ggaaaccagg caagtaaata
     5341 cacctttacg ttacactaaa tgagaacgta tttgcggcga taaagtttct taatttttat
     5401 taaataaatc ctttgcactt ggcgattgac gctcactttc gggaattgcg agcacttctt
     5461 tgcggcgata gttcatcatt cctgccatga tattggttgg gaacatctcc acgctattat
     5521 tatacgcggt aactgctgca ttgtaagttc tgcgggctgc cgagagctgc tcttcaattt
     5581 ctgtccaagt gctttggagt tgaataaagc tgttattcgc tttcaaatcg ggatagtttt
     5641 caacggcaac acgcaattgg gtaagcaatc ccgtaagctt attttctgca tccatacgat
     5701 cctgaacagg catgcccgga ttggattgta cgctgttgcg gagttctacg atacgggtga
     5761 gggtatcctg ttcataggtc atgtattgct tcactgtttc aaccagattc ggcagcaaat
     5821 cgaaacgctt tttcaacatg gcatctatgg taccgaaggc gttctcgaca ccatttttac
     5881 gacttacaag tgtgttataa agtccgatca ggattaaggc taaaacgcct ataataatta
     5941 ataccggtac tagcatggtt gtgtgatttt aatgaccaaa attaaataaa atgcaatagg
     6001 ttgaggtata atttttagct gaagcaatca aaacggcata aataccggca cttaaggaaa
     6061 taatttgttg ccatgccgat ttacattacc tcttccggca gttgagcaat ggtatccatt
     6121 acttccatca gcgctccaac atccattaag tttaccagct cgttgcgata ggatttgata
     6181 ttcggtatgc ctttgtagta attggtatag tgtcgacgca tttccagaat gccgagcact
     6241 tcacctttcc attctaccga ctttagcaga tgtttcttac atacctctac gcgttcctcc
     6301 atggtaggcg ccgcaaggtg ttcgccggta tttaagaagt acttaatctc acgaaacacc
     6361 catggatgcc cgatcgcagc tctgccaatc atgatgccat cgacaccata agtatttttc
     6421 atttccagtg ccttttccgg tgtatctaca tcaccattgc cgaaaatggg gatctgaata
     6481 cgtggattat tctttacagc acctatcaat gtccaatcgg cagaaccttt atacatctgg
     6541 gcacgtgtac gaccatggat ggtcagggct ttgatgccga tatcctgtaa gcgttcagca
     6601 acttccatga tgttcttgga gttatcatcc catccaagtc tggtttttac ggtaacgggg
     6661 agcttggtag cctttacgat ctcggcggtg aggcgaacca tacgcggaat atccttgagg
     6721 atacctgcac ctgcatcttt gcacactact tttttcaccg gacagccgaa attgatgtcg
     6781 agcaggtcgg gaccggcttc ttcaacgatc tgggcgcagc ccttcatcac atcgatatcg
     6841 gcaccgaata tctgtatgcc tattgggcgc tctgcttcat agaattcaag ttttttggtg
     6901 ctcttttcgg catgacggat gagcccttca gtagagatga actcagtgta catgaggtcg
     6961 gcaccttgtt ctttgcaaag cacacggaat ggcggatcac tcacgtcctc c
//
LOCUS       DAOQHY010000161         6984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_5723_length_6984_cov_5.459518, whole genome shotgun sequence.
ACCESSION   DAOQHY010000161 DAOQHY010000000
VERSION     DAOQHY010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6984
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5723_length_6984_cov_5.459518"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1862
                     /locus_tag="PLL28_11760"
     CDS             <1..1862
                     /locus_tag="PLL28_11760"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNF70045.1"
                     /translation="IGDASTSEGLFWESINAAGVLKIPMAVFVWDDGYGISVPQKYQT
                     TKQNISEILSGFQLDEDGHGVRIYEVKGWDYPSMVQTFEEGIDLCRREHIPVLFHVTE
                     ITQPQGHSTSGSHERYKSKERLEWEAEWDCVRQMRKWMIAASVATEEELNDIVAKAKD
                     EARQHQKAAWNAHLMSIKTDMDVVFSLLENLRNSSNASGQIAAIITELKDTMDAVRKD
                     IMRAARLALHATAGEQTIERAALQAWAEDYAALNDQRYNGNLFSNSAHAIQHIPVIPA
                     QYPEEPTMVNGFELLNKCFDMHFSTNPRIYAFGEDVGKIGDVNQGFAGLQEKHGEHRI
                     FDVGIREATIIGQGIGMALRGMRPIAEIQYLDYLIYGLQPISDDLATLQYRTAGGQKA
                     PLIIRTRGHRLEGIWHTGSPMGMILNTCKGVHLCVPRNMTQAAGMYNTLLQSDEPAIV
                     VECLNGYRLKEALPLNINTFTVPLGVPEILRNGTDITVVSYGSTLRVVEEACDRLAAL
                     GMSIELIDAQTLMPFDVQGIIAASVRRTNRVLFVDEDVPGGASAYMMQQVFADETLFR
                     SLDAFPKCLSAKANRSAYASDGDYFCKPSEEDVFEAIYAMVSEVDPGQYPAIS"
     gene            1917..2111
                     /locus_tag="PLL28_11765"
     CDS             1917..2111
                     /locus_tag="PLL28_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HNF70046.1"
                     /translation="METGKIKFFNATKGYGFITKDGTNEEIFVHQTGLVDKVKQNDLV
                     TFDIIDGRKGKNAVNVKLAQ"
     gene            complement(2441..3160)
                     /locus_tag="PLL28_11770"
     CDS             complement(2441..3160)
                     /locus_tag="PLL28_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNF70047.1"
                     /translation="MGRAFEYRKERKFKRWGAMATAFTRVGREIAIAVKNGGPNPEAN
                     PKLRIAIQNAKAVQMPKDRVEAAIKRASSKDEKDFEEVVYEGIGPGGVAIMVECATDN
                     TTRTVANVRVLFSKGGGALGTSGSVGYLFERKGIFKVESGKIKLDVVELDLIDGGAED
                     IYEEDGTVYIETSFNDFGNMQKKLDELNIEISGAELLRIPMNKNAVPPDAEKLVHNLI
                     EKLEEDEDVVNVFHNMVETME"
     gene            complement(3217..5499)
                     /locus_tag="PLL28_11775"
     CDS             complement(3217..5499)
                     /locus_tag="PLL28_11775"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNF70048.1"
                     /translation="MKSSLLIFYLCLLSTFVAKAQMTPPYSILYDDTQVNKIYITMDI
                     DSLSEMYDELENEHEYAVQFVFDRGTQTDTLQDVGFRLRGNTSLSSAKKSFKVSFNTY
                     NPGRTFETAKKLNLIGNHNDPTMVREKTYFDIYNRLGLPMRRVGFAEVYINDQYFGFY
                     TVVEEYDDIWCRDRYGDDAGALFKCLYGSTFTYNGENTGAYNTYEQQSNEQVNMKGEL
                     AHMFDVLNNTPIADLPCAFEPLFDVDQFLKIYALDISTGHWDNYGANQNNYYLYHDLK
                     SGQYKFLSYDCDNVLGIDWFGIDWGTRNVYDWNFDGRPLVERLLQVDVYRERFSYYLN
                     LIATYGMDTDYWYPRLDDIRDLIAPSALADIYRTMDYGYSYGDFLDGFDTDDIDGHCP
                     YGIKNFFDTRINSTLDQVELSDIAPIIHFPQQDHMLLEHGDYLNITVFIEEDVSVSAV
                     QLLYSNNETGYSTADLYDDGAHGDGIAGDNIYGNTVIVPMDGTTFYYHIIASDNTGNE
                     SVYPVCENIARTIGYTTPTLHINEVMPDNVSTVPDGAGEFPDFIELYNYGLYAIDISN
                     LFITDELDRPTKWRLPDMMLPFDSYVLIFADNETEQGLNHATFSIDADKDDLFIYGDV
                     TTDYYLIDSTSFKNIPPDVSWGRLPNGTGPFTNLPVASPGAYNTVDTASVPDLFEVIL
                     TNNPGTHTSEVIITNDIAQTITLHIFNMAGQEVFKEAPATLPAGTYSYSIPANRLGSG
                     AYLIRIGHGDKVTMITYSVL"
     gene            5529..>6984
                     /locus_tag="PLL28_11780"
     CDS             5529..>6984
                     /locus_tag="PLL28_11780"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HNF70049.1"
                     /translation="MKHKLLLIASICMLRALPSGAQTPAWAKEAIWYQIFPDRFRNGD
                     PSNDPTLADIKGCWPHNDTAAWEIMPWTSDWYAMQPWELANGQDFNYNVQRRRYGGDL
                     QGIIDRLGYIDSLGINAIYLNPVFWAPSLHKYDAVMYHHVDPNFGPDPEGDKKLIASE
                     DPINPATWHWTAADKLALQLINSCHKRGIKIIFDGVFNHMGINSFAFQDVVKNQQDSK
                     YKDWFTIYSWDDSLAGTTFKYQGWFGVPDLPEIKEDSMGIVAGPKQYIFDCTKRWMAP
                     DGNAANGIDGWRLDVAFCVDHDFWKSWRLWVKSINPEAYLTAEVIDKKEVVAPYLQGD
                     EFDAVMNYGFGVIASEFFINKQSRISVTTFDSLLADLRQAYRPDFTYVMQNLYDSHDT
                     QRFTSFLHNPDIARFHTWGEYFNATKSTNLAYNVGKPTEEEYKRQMLMALFQYAYVGA
                     PMLYYGDEVGMWGANDPDCRKPMVWQDMNYQPEQK"
BASE COUNT         1968 a         1388 c         1617 g         2011 t
ORIGIN      
        1 ctatcggcga tgcgagtact tcagaaggcc ttttctggga gtcgataaac gctgcaggtg
       61 tgcttaagat accaatggcg gtttttgtgt gggatgatgg ttatggtatc tccgtgccgc
      121 aaaaatacca aactaccaag cagaacattt ctgagatact ctcagggttt cagctcgacg
      181 aagatggcca tggcgtgcgc atctatgagg taaaaggctg ggactaccct tcgatggttc
      241 agacctttga ggagggtatt gacctttgcc gcagagaaca tattccggta ttatttcatg
      301 ttactgaaat cacccagcca caaggacatt ctacttcagg ttcacatgag cgctacaaaa
      361 gtaaggagcg cctggaatgg gaggccgaat gggactgtgt gcgtcagatg cgtaaatgga
      421 tgatagccgc cagtgttgca acagaagaag aactcaacga tattgttgcc aaagcaaaag
      481 acgaggcccg tcagcatcaa aaagctgcat ggaatgcaca tctgatgtct ataaagacag
      541 acatggatgt agttttttcg ctactggaaa acctccggaa ttcaagtaat gcctccggac
      601 aaatagcagc aatcattact gagttgaaag atacgatgga tgctgttcgt aaagatatta
      661 tgcgtgccgc tcgattggca ttgcatgcta cagccggaga acagaccatt gaacgggcag
      721 ctttacaagc atgggctgaa gactatgcgg cattgaatga ccaacgctat aatggaaatc
      781 tcttcagcaa ttcagcacat gccattcaac atattccggt gattcccgca caatatccgg
      841 aggagccgac gatggtaaat gggtttgaac tgttgaataa gtgctttgat atgcatttct
      901 ctacaaaccc ccgcatttat gcctttggtg aagatgtggg caagattggt gatgtgaatc
      961 agggttttgc aggactgcag gagaaacatg gagaacaccg catttttgat gtgggtattc
     1021 gcgaggcaac cattattggg cagggaatag gcatggcatt gcgtggcatg cgtcccatcg
     1081 ctgagataca atacctcgat tatctgattt acggcttaca acctatcagc gacgacctgg
     1141 cgactttgca gtatcgcacg gcgggtgggc agaaggcgcc tttgattatt cgcacacgcg
     1201 gacataggct tgaaggcatt tggcataccg gttcacctat gggaatgatt ttgaatacct
     1261 gcaagggcgt tcacctttgt gttccaagaa atatgacaca ggctgcgggt atgtataata
     1321 ctttgcttca aagtgatgag cctgctatcg tggtggagtg cttgaacggc tatcgcctga
     1381 aagaggcgct gccattgaat atcaatacgt tcactgtgcc gctgggtgtg cctgagattc
     1441 taaggaatgg aacagatatc actgttgtca gctatggttc tactcttcgt gtggtagaag
     1501 aagcctgcga cagactggct gcgctgggta tgtcaattga actcatagat gctcaaacac
     1561 tgatgccatt tgatgttcag ggcattattg cggcatctgt tcgtcgaaca aatagggtac
     1621 tgtttgtaga tgaagacgtt ccgggtggag cttcggccta tatgatgcag caggtatttg
     1681 cagatgaaac cctattccgc tcccttgatg ccttcccgaa atgtttgagc gcgaaagcca
     1741 acaggtcggc ttatgccagc gatggtgatt atttctgtaa gccaagcgaa gaggatgtgt
     1801 tcgaagctat ctatgctatg gtcagcgaag tggatccggg gcagtatccc gcaatttctt
     1861 aaagtttata tatctttgaa cgttaaccaa tttttttact actaattatt attacaatgg
     1921 aaaccggcaa aatcaaattt ttcaatgcca ctaagggtta tggtttcatc actaaggatg
     1981 gtaccaacga agaaattttc gttcaccaga ccggtctggt tgacaaggtt aagcaaaacg
     2041 atcttgttac ttttgacatc atcgacggtc gcaaaggcaa aaacgcagtt aacgttaaac
     2101 ttgcgcaata atcttattga ctattcagtc gggaagctca ttcgtaagaa tgggcttttt
     2161 ttttggtgag tggtgagtgg gtgagtggtg agtggtgagt ggtgagtggg tgagtttacc
     2221 tcgctgagca ggcgggtggt gagtttgggg taagacgtga atcgtgactc gtgaatcgtg
     2281 actcgtgagt ttggggtaat acgtgaatcg tgactcgtga gtttggggta atacgtgaat
     2341 cgtgaatcgt gactcgtgag tttggggtaa tacgtgactc gtgagtttgg ggaatttgtt
     2401 ttgcgccttt gaaactttgc gtcttagcgc gaaaaaaaac ttactccatg gtttccacca
     2461 tattgtggaa tacattcact acgtcttcgt cttcttcgag tttttcgatg agattgtgca
     2521 cgagtttttc agcgtcggga ggaaccgcat ttttattcat gggaatacgc aataattcgg
     2581 caccactgat ctcgatattc agttcatcaa gtttcttctg catatttccg aaatcgttga
     2641 aagatgtttc gatgtaaaca gttccatctt cttcataaat atcttcggca ccaccatcga
     2701 tgaggtctaa ttcaacaaca tcgagtttaa tctttccgga ttcaactttg aaaatacctt
     2761 ttcgttcaaa taaataacca actgatccgc tggtacccaa agcacctcct cctttagaga
     2821 agagtacgcg aacatttgca acggtacgag tagtattatc ggtagcacat tcaaccatga
     2881 tggcaacacc accggggccg ataccttcgt ataccacttc ctcaaaatct ttttcatcct
     2941 tgcttgatgc gcgtttgata gcagcttcta cacggtcttt cggcatctgt accgccttag
     3001 cattttgaat ggcaatacgc aacttgggat tcgcttcagg attaggtccg ccgtttttta
     3061 cagcgatggc aatttcacgt cctacacggg taaaggcagt agccattgca ccccagcgtt
     3121 tgaattttct ttccttgcga tattcgaatg cgcgtcccat aataattgtt ttgaggcagc
     3181 aaatttacgg ctttagccga caattggcaa ttatcattat aatacagagt atgtaatcat
     3241 tgtaacctta tcgccatggc caatacgaat caggtatgca ccggaaccca ggcgatttgc
     3301 aggaatgctg taactatagg tgccggcggg caatgtggca ggtgcttctt taaatacttc
     3361 ctgccctgcc atgttgaaaa tatggagtgt aatggtttgg gcaatatcgt tggtaatgat
     3421 tacctcagaa gtatgcgttc ccggattatt ggtcagtatt acttcgaaca aatccggcac
     3481 ggaggcggtg tccaccgtat tataagctcc cggggaagca actggtaaat tcgtaaatgg
     3541 accagttcca tttggtaatc tgccccatga aacatcaggt ggaatattct tgaatgaggt
     3601 ggaatcgatt aaataataat cggtagtgac atcgccataa ataaataagt catccttatc
     3661 tgcatcgata gagaaggtag catgattgag gccctgttcc gtttcattat cggcaaagat
     3721 taacacataa ctgtcaaaag gaagcatcat atcaggcagt cgccattttg ttggcctgtc
     3781 caattcatcg gtgataaata aattgctgat gtcaatggca tataaaccgt aattatataa
     3841 ttctataaaa tcaggaaatt caccggctcc atcgggcacc gtgcttacat tatcaggcat
     3901 cacttcgttg atatgtaatg ttggtgttgt atatccgatt gtgcgtgcga tgttttcaca
     3961 aacaggataa acactttcat ttcctgtatt atcagatgcg ataatatgat aataaaaagt
     4021 ggtgccatcc ataggtacaa ttacggtgtt accataaatg ttatcacccg caatgccatc
     4081 accatgtgca ccatcgtcat ataaatctgc agtgctgtaa ccagtttcat tattactata
     4141 caataactga acagcggata cggaaacatc ttcttcaatg aaaacagtga tatttaaata
     4201 atcaccatgt tctaaaagca tatgatcttg ttgggggaaa tgaatgatag gtgcgatatc
     4261 acttaattcc acctgatcaa gtgtgctgtt gatgcgtgtg tcgaaaaaat tcttgatacc
     4321 gtaagggcaa tgtccgtcta tatcatctgt atcaaaacca tcaagaaaat cgccatacga
     4381 ataaccataa tccatggtgc gataaatatc agctaatgct gacggagcga taaggtcgcg
     4441 gatgtcgtct aaacgcggat accaataatc ggtatccatg ccataagtgg caatgaggtt
     4501 gagataatag ctgaaacgtt cgcgatatac atcaacttgc agcaatcgtt caactaatgg
     4561 tcggccatca aaattccagt cgtaaacatt gcgtgttccc cagtcaattc cgaaccaatc
     4621 gatgccaagg acgttatcgc aatcatagct caggaattta tattgccctg attttaaatc
     4681 atggtagaga taataattat tttgattggc gccataatta tcccagtgac cggtagagat
     4741 atctaatgca tagatcttta aaaattgatc tacatcaaat aagggttcga aagcacaagg
     4801 cagatcggca atgggtgtgt tattcaatac atcgaacatg tgagccaatt cgcctttcat
     4861 attcacttgt tcatttgatt gttgttcgta tgtgttgtat gcacctgtat tttctccatt
     4921 ataggtaaat gtactgccgt ataaacattt gaacagagca cctgcatcat cgccatagcg
     4981 gtcgcggcac cagatatcgt catattcttc aactacggta taaaatccaa aatattgatc
     5041 gttaatatac acttcagcaa atcccactct gcgcatggga agccctaggc gattataaat
     5101 gtcgaaatac gttttttcac gaaccatagt agggtcgtta tgattgccga taagatttaa
     5161 tttcttggca gtttcaaacg tgcgacccgg attgtaagta ttgaacgaaa ctttaaatga
     5221 tttttttgca ctggataaag aggtattacc tctcaatcta aatcctacat cctgtaaggt
     5281 gtcggtttgt gtacctctgt cgaaaacaaa ttgcactgca tactcatgtt cgttttcaag
     5341 ttcatcgtac atttctgaaa gcgaatctat atccatggtg atatagatct tgttcacttg
     5401 tgtgtcgtca tataaaatac tatagggtgg tgtcatttga gcctttgcaa cgaaggtgct
     5461 gagcagacac agataaaata tgagtaacga acttttcaat gcagcactta tttatgcaaa
     5521 tttacactgt gaaacataaa cttctgttaa ttgcatcaat ttgtatgcta agggcgcttc
     5581 catcaggtgc tcagacccct gcatgggcaa aagaggcaat atggtatcag attttccccg
     5641 accgctttcg caatggggac ccttcaaacg atcccacgct tgccgatatc aaaggttgct
     5701 ggccgcataa tgatactgca gcctgggaaa tcatgccatg gacatcagac tggtatgcca
     5761 tgcaaccgtg ggaactcgcc aatggacaag attttaacta caatgtgcaa cgcaggcgat
     5821 acgggggcga tttgcaaggg attatcgatc gcttagggta tatcgacagt cttggcatta
     5881 atgccattta tctgaatcct gttttttggg cgccgagttt gcataagtat gatgcggtaa
     5941 tgtatcatca tgtagatccg aattttggtc cagatcctga gggtgataaa aagttgatag
     6001 caagtgaaga cccgataaat cctgcaacct ggcattggac ggcagcagat aaactggcac
     6061 tgcaattaat aaactcctgt cacaaacgcg gtattaaaat cattttcgac ggcgtgttta
     6121 atcatatggg cataaatagt tttgcttttc aggatgtggt aaaaaatcag caggattcta
     6181 aatacaaaga ttggtttacc atttatagtt gggatgatag ccttgcagga accacattta
     6241 aatatcaggg ttggtttggt gttccggatc tccctgaaat aaaagaagat tcgatgggaa
     6301 ttgtggccgg tccgaaacaa tatatttttg actgcaccaa aagatggatg gcacctgatg
     6361 ggaatgcagc aaacggtata gacggatggc ggttggatgt ggctttttgt gtggatcacg
     6421 atttttggaa atcatggcgc ttgtgggtga aatctattaa tccggaagca tatttaactg
     6481 cggaagtaat tgataaaaag gaagtagttg caccttattt acaaggagat gaatttgatg
     6541 cagtaatgaa ttatggattt ggtgtgattg caagtgagtt ttttataaac aaacaatcgc
     6601 gcatttctgt aaccaccttc gattccttac ttgctgattt acgtcaggcc tatagaccgg
     6661 atttcactta cgtaatgcag aatttgtatg atagtcacga tacgcagcgc ttcacatctt
     6721 ttttgcataa tccggatatt gcgcgttttc atacttgggg tgaatatttt aatgcaacta
     6781 aatcaacaaa tctcgcatat aatgtaggca agcccactga agaggaatac aaaaggcaaa
     6841 tgttgatggc gctttttcaa tatgcttatg taggcgctcc gatgttgtat tacggcgatg
     6901 aggtaggcat gtggggagca aatgatccag attgccggaa gccaatggta tggcaggata
     6961 tgaactatca gcccgagcaa aaat
//
LOCUS       DAOQHY010000162         6983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_5727_length_6983_cov_6.005918, whole genome shotgun sequence.
ACCESSION   DAOQHY010000162 DAOQHY010000000
VERSION     DAOQHY010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6983
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5727_length_6983_cov_6.005918"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..172)
                     /locus_tag="PLL28_11785"
     tRNA            complement(100..172)
                     /locus_tag="PLL28_11785"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(137..139),aa:Gly,seq:gcc)
     gene            332..1993
                     /gene="ftcD"
                     /locus_tag="PLL28_11790"
     CDS             332..1993
                     /gene="ftcD"
                     /locus_tag="PLL28_11790"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HNF70050.1"
                     /translation="MRQIIECVPNFSEGRDMQIIRQITDAISSVEGVTLLDVDPGKAT
                     NRTVVTFVGDADAVVEAAYRGIAKAAEVIDMRKHHGEHPRMGATDVCPFVPVANATME
                     DCIACAKKLGERVGSTLNIPVYLYESAATSPERKNLATIRAGEYEGIEKKILDPMWKP
                     DFGPAQFNPQTGNCIIGARDFLVAFNVNLNTTSVRRANSIAFDVRENGRVDPSGAKDA
                     QGEALRIPGACKSVKAIGWFIEEYGIAQVSMNLTNIRDTPVHIAFDACVNSAASRGMR
                     VTGSELVGLIPLQCLIDAGKYFLEKQERSIGVSEEELIKIAVKSMGLDELAPFNPADK
                     IIEYKLGSTTGKLVSMNLRAFANETASESPAPGGGSIAAYCGALGISLATMVANLSSH
                     KKGWDARWKEFSDVADKGQQLKDRLLFMVDEDTAAFNKIMDAFGLPKATPDEKSARKK
                     AIQDATVYAIEVPFKVMQLSFESMALIKAMAENGNPNSVSDAGVGALCARTAVLGAFL
                     NVKINASGLDDKALAANKIAMAQKIADDAMAMEKEILSIVDSKIG"
     gene            complement(2006..3094)
                     /locus_tag="PLL28_11795"
     CDS             complement(2006..3094)
                     /locus_tag="PLL28_11795"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70051.1"
                     /translation="MQKAKEIISTYDGKQPLQLHLKNYFRLHKEMGSRDRKLYTQLAF
                     GYYRQKGMAPHNDRFLVDAVANMEQFHPFYAYWSAEVPEVAGIAWNQYFPFSDHISDS
                     INIEELEKSLHTNPATWIRCKKAHITDILDELSAKGFVFDRDDTCIRFDQPVPLQELE
                     TFEKGYFEIQDIASQQTGTLMHPEKNAAWWDCCAGSGGKSLLLLEKEKQLQLFVSDNR
                     ESILHNLQDRFSRAGIRQFAAKLIDLETADDKALQGLPDMQGIIADVPCSGSGTWART
                     PERMAHFDGTQLEKFVALQHKILDRTVNKLLPGGTYLYLTCSVFTDENEQQVAYLQQQ
                     HQLTLQEMHYFQYSSQGGDTLFGARFTK"
     gene            complement(3125..3349)
                     /locus_tag="PLL28_11800"
     CDS             complement(3125..3349)
                     /locus_tag="PLL28_11800"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HNF70052.1"
                     /translation="MSIEKHTYTNGEVTIVWQPKLCIHSGNCVRGLPAVFNNRERPWI
                     KAEGADTEAIIEQVKKCPSGALTFILNTNK"
     gene            3434..4240
                     /gene="murQ"
                     /locus_tag="PLL28_11805"
     CDS             3434..4240
                     /gene="murQ"
                     /locus_tag="PLL28_11805"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HNF70053.1"
                     /translation="MDKITEQPSRYRHLEKMSTTELLSNINTEDKSVPLAIEKVLPQI
                     AQLVDVVADKMLAGGRLFYIGAGTSGRLGIVDASECPPTFGVPHGLVIGLIAGGDAAI
                     RKAVERAEDDMEQGWLDLMAYQIGPNDVVVGIAASGTTPYVIGALKTCREKGITTACI
                     TCNTGSPVAMQSDFPIEIVVGPEFVTGSTRMKSGTAQKLCLNMLSTAVMIRLGRVEDN
                     KMVNMQLTNDKLVDRGTKMLAELLDIEYEEAKKQLLLHGSVKKAVQAHSK"
     gene            4326..4895
                     /locus_tag="PLL28_11810"
     CDS             4326..4895
                     /locus_tag="PLL28_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70054.1"
                     /translation="MQEIEPYYNWREYYVSEEDENSPFFGREYSEFEFSNTIYNFFIH
                     PQWDEFGSATLYMKIIFADYDSGVAIMEFIGEWNDCLYNDIMFLKRDVIDELKYAGIH
                     KFILIGENVLNFHGGDEDYYEEWFDEIEDGWVACLNFREHVEAEMISAHIDRYFLIGA
                     PFNDINWRKYKPQALAFLVEQYMQKKIGA"
     gene            complement(4898..5626)
                     /locus_tag="PLL28_11815"
     CDS             complement(4898..5626)
                     /locus_tag="PLL28_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70055.1"
                     /translation="MELATLKLGNLIIGEPMTSLTDILLAVTSFVLVARIRNCLNESF
                     FNNAWRMFFLCMGISTSIGTIAHAINGSSAQQLFNILWMAMTFASSTAVFYAVHATIR
                     FMRSSGQWRKIFMAINVIALIVFLLYTAAFNNFEIFKIHAAVGVVIIFLTHTIAWLRN
                     HHGSGWVAVGMGLSILTVVIHSMQLSISKWFNYKDISHVIMLVSLICIYNGIHAMSDN
                     LQLSVFRSRTKESENNFNGAERAL"
     gene            complement(5717..>6983)
                     /locus_tag="PLL28_11820"
     CDS             complement(5717..>6983)
                     /locus_tag="PLL28_11820"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNF70056.1"
                     /translation="NDSLFTTLDSPDQGVNDIPTSSTTYTAGSNEIQVEIPLIKAQFK
                     GVYTAATQSISGVFIQGAPMPMTLTKVTEVKGIVRPQEPVKPYPYKEEEVTFKNKQTN
                     DVTLAGTLTIPEGKGPFTAVILVTGSGPQDRNEALLGHKPFLVLSDYFTRKGIIVLRY
                     DDRGVAQSTGDHETATTADFASDANAAVDYLLSRKDLNIGKIGIAGHSEGGVIAPLAA
                     SKNKQIDFIVLLAGTGIPGDSILNLQADLIARAMGAPEDALQATEKYRRDIINIAKAN
                     DDPAMIKSAIIAYNKNLIKNSSAEYLNLLSMTETDTLAAVNFYANAWMHYFLNYDPQP
                     TLRKLKIPVLAVNGTHDLQVPYKENLSAIEAALKEGKNKQYSTMAFDGLNHLFQQSAT
                     GSPSEYSHIEETFNEQAMEAIATWILTLK"
BASE COUNT         2004 a         1402 c         1558 g         2019 t
ORIGIN      
        1 ggacattact gcccacagga ttttaagtcc tgcgtgtcta ccgattccac cacccgggcg
       61 ccttaaaaaa ggtaagcacg gctgttgccg tgctgtatga gcgagagacg agattcgaac
      121 ccgcgacccc aaccttggca aggttgtgct ctaccagctg agctactctc gcatttccct
      181 ttaatcggga gtgcaaatat ataaggattc ggggtatccg caaaaatgta agcgaaaaaa
      241 atctgtaagt cgccgccggt caatggttta cgataattaa tatcatcaat gcctatggcg
      301 cttatgccct atatttgagc aaaattgtac catgcgccag atcatagaat gtgtgcctaa
      361 ttttagcgaa ggcagagata tgcaaatcat ccgtcagatc accgatgcga tttcttctgt
      421 tgagggtgtt accctcctgg atgttgaccc ggggaaagct accaatagaa ctgtagtgac
      481 ctttgttggc gatgccgatg ccgttgttga agctgcatat cgaggaattg ccaaggctgc
      541 tgaggtaatt gatatgcgca aacaccatgg cgagcatccc cgcatgggcg ccacggatgt
      601 atgtcctttc gtgcccgttg ccaatgccac aatggaagat tgtatagcct gcgcaaaaaa
      661 attaggtgaa cgcgtaggat ctacattgaa tatccctgtt tacttgtatg aaagtgccgc
      721 aacctctcct gaaagaaaaa atctggcaac cattcgcgcg ggtgaatatg agggtattga
      781 aaagaaaatt ctagatccca tgtggaaacc cgacttcggt ccggcacaat tcaatcccca
      841 aaccggaaat tgtattatcg gcgcaagaga ttttcttgtt gcctttaatg taaacttaaa
      901 tactacttct gtacgtcgcg ctaactccat tgcgttcgat gtgcgtgaaa atggcagggt
      961 agatccatca ggtgctaaag atgcacaagg tgaagcactg cgcattcccg gtgcatgcaa
     1021 aagtgtgaag gcgatcggtt ggtttattga agagtatgga attgcacagg tttcaatgaa
     1081 cctcaccaat atccgcgata cgcctgttca catagccttc gatgcatgtg tgaacagcgc
     1141 agcatcacga ggaatgcgcg taacaggaag tgaattagta ggattaattc ctttgcaatg
     1201 tttaattgat gcgggaaaat atttcttaga aaaacaagaa cgctcaatag gtgtcagcga
     1261 agaagaatta ataaaaatcg cagtgaaaag tatgggtctc gatgagctgg ctccgtttaa
     1321 tccggcagat aaaattatcg aatataaact cggctctact accggcaaac tggtgtcgat
     1381 gaacctgcgt gcttttgcca atgaaacggc atctgaaagt ccggcgcccg gtggtggctc
     1441 tatcgctgca tattgtggag cattgggtat ttcactggct accatggttg ccaatttatc
     1501 gtcacacaaa aaaggttggg atgctcgttg gaaagagttt tcagatgttg cagacaaagg
     1561 acagcaactg aaagatcgcc ttttatttat ggtggatgaa gataccgctg ccttcaataa
     1621 aatcatggat gccttcggct tgccaaaagc taccccggat gagaaatcgg caaggaaaaa
     1681 agccattcag gatgctaccg tttatgctat agaagtgcca tttaaagtaa tgcaacttag
     1741 tttcgaaagt atggcattga taaaggcgat ggctgaaaat ggaaatccga attcagtgag
     1801 cgatgccggt gttggtgcat tgtgcgcaag aactgctgta ctaggtgcct tcctgaatgt
     1861 aaaaattaat gcatcaggtc tcgatgataa agcacttgcc gcaaataaaa tcgccatggc
     1921 tcagaaaatt gcagacgacg caatggcaat ggaaaaagaa atattatcca ttgttgacag
     1981 taaaataggg tgattaaata atatgttatt ttgtaaacct tgcaccaaac aaggtatctc
     2041 ctccttgtga tgaatattga aaatagtgca tctcttgcaa tgtcaattga tgttgctgtt
     2101 gaagataggc aacttgctgt tcattctcat cggtaaatac ggagcaggta agatataaat
     2161 aggtgccacc cggcaataat ttatttactg ttcgatcaag aatcttgtgt tgcagcgcaa
     2221 caaatttctc taattgtgtg ccatcaaaat gtgccatccg ctcgggtgtg cgtgcccagg
     2281 ttccggatcc gctgcatggc acatcagcaa tgattccctg catatcagga agaccttgca
     2341 aagctttgtc gtcggcagtt tccaaatcga tcagcttggc tgcaaattga cgaataccgg
     2401 cgcgtgaaaa tctgtcttga aggttatgta aaatactctc cctattatcg ctcacgaata
     2461 actgtaattg tttttctttt tcaagcaaca ataaactctt tcctcctgaa ccggcgcagc
     2521 aatcccacca cgcagcattt ttttcgggat gcatgagtgt tcccgtttgc tgcgaagcga
     2581 tgtcttgtat ttcaaaatat cctttttcaa aagtttctaa ttcctgcaat ggaacgggtt
     2641 gatcaaaacg aatgcaggtg tcatcgcggt caaatacaaa tccttttgca cttaattcat
     2701 cgagaatatc tgtaatatgt gcttttttac atcgtatcca ggttgcagga tttgtatgta
     2761 gacttttttc cagttcttca atatttattg agtcggaaat gtgatcgcta aatggaaagt
     2821 attgattcca cgcaatgccg gcaacttcag gaacctctgc cgaccaataa gcatagaacg
     2881 gatgaaattg ttccatattg gcaacggcat ctacaagaaa cctatcgtta tgcggcgcca
     2941 tgcctttctg tcggtaatat ccgaaggcaa gttgcgtata aagcttgcga tctctgctgc
     3001 ccatttcctt atgcaggcgg aaataatttt taaggtgcaa ttgcaaaggt tgttttccat
     3061 cataagtaga aatgatttcc tttgcttttt gcatttgaac tatttcgcgc atattgaaac
     3121 gatattattt gtttgtattc aaaataaaag tcaatgcacc tgagggacat ttctttacct
     3181 gctcaataat agcttccgtg tcggcgcctt ctgcttttat ccagggacgt tctcgattgt
     3241 tgaatacagc aggcaatcct cttacgcaat tgcccgaatg gatgcagagt tttggttgcc
     3301 atacaatggt cacttcacca ttggtataag tatgtttttc gattgacata gtacctgatt
     3361 attaagcaag tatacataaa acccaatcaa atcgcctgag gataatgcaa atttgattta
     3421 ttttgtatca caaatggaca aaataaccga gcaaccatcg cgatatcggc atctcgaaaa
     3481 gatgtcaacc acggagctat tgagcaatat caatacggaa gataaatcgg ttccgcttgc
     3541 catagagaag gtgctgcctc aaattgctca attggttgat gtagtagctg ataaaatgct
     3601 ggccggtgga aggttatttt acatcggtgc aggaacatca ggccgtcttg gcattgttga
     3661 tgcgagtgaa tgtccgccaa ctttcggtgt tccgcatgga ttggtgatag gattaattgc
     3721 aggaggagat gccgccatca gaaaggctgt tgaacgggcc gaggacgata tggagcaggg
     3781 ttggctcgat ttaatggcct atcaaatcgg gccaaatgat gtggttgttg gcattgccgc
     3841 cagcggaaca acaccatatg tgatcggtgc attaaaaacc tgtcgcgaaa agggaattac
     3901 aacggcttgt attacgtgca ataccggttc accggttgcc atgcaatcag actttcccat
     3961 cgaaatagtt gtaggtcccg agtttgttac cggctctaca cgcatgaaaa gcggaaccgc
     4021 tcagaaactc tgcctcaata tgctctctac ggcagtcatg atacgtttag gcagggtaga
     4081 agacaataaa atggttaaca tgcaattaac caacgacaag ctggttgatc gcggaaccaa
     4141 aatgttggca gagttattgg atattgaata tgaggaggcg aaaaagcaat tgctattgca
     4201 tggaagtgtt aagaaagcag tacaggcaca tagcaaataa gtcgatttta acttcctttt
     4261 tttagttgca ttaattttat ttttttgttt tagaaatacg gattattttt ccggcgttat
     4321 caggtatgca ggagattgaa ccatattaca attggcgtga atattatgtg agcgaagaag
     4381 acgagaatag tcctttcttt ggtagggaat atagtgagtt cgaattttca aatacgatat
     4441 ataatttttt catccaccct caatgggatg agtttggctc tgcaacatta tatatgaaaa
     4501 ttattttcgc cgattatgat tcaggtgtag ccatcatgga atttatcggc gaatggaatg
     4561 attgcctgta caatgatatc atgtttttaa aacgcgatgt aattgatgag ctaaaatatg
     4621 caggcatcca taagttcatt ttgattggtg aaaatgtttt aaactttcat ggtggcgatg
     4681 aagattacta tgaagaatgg ttcgatgaaa ttgaagatgg ttgggtagct tgtttgaatt
     4741 tccgagagca tgttgaagca gaaatgataa gtgcccacat cgatcgttat tttttgatag
     4801 gtgctccgtt taacgacatc aactggcgta aatacaaacc acaggcatta gcctttttgg
     4861 tagaacaata catgcaaaag aaaataggtg cctaatttca aagagccctt tcagcgccat
     4921 taaaattatt ctcagattcc ttagtccgtg agcggaatac actcaattgc agattgtcgc
     4981 tcatggcgtg aatgccgtta tagatacaga tcaggctcac cagcataatt acatggctga
     5041 tgtctttata attaaaccat ttagatattg aaagttgcat gctgtgaatc accactgtta
     5101 aaatggaaag acccatgcct acagcaaccc agccacttcc gtgatgattg cgcaaccagg
     5161 caatggtatg tgttaggaaa attattacca caccaacggc ggcatgaatt ttaaatattt
     5221 cgaaattgtt gaaagcggcg gtatacaata aaaaaacgat aagcgcaatc acatttattg
     5281 ccataaatat ttttcgccac tgtcctgaag aacgcataaa tctgatggtg gcatgaacag
     5341 cataaaacac ggcagtgctg ctggcaaagg tcattgccat ccacaaaata ttaaataatt
     5401 gttgtgcact agatccgttt atggcatgcg ctattgtccc aatggaggta gaaattccca
     5461 tacataaaaa gaacatgcgc caggcattat tgaaaaagct ttcatttaag cagttgcgta
     5521 ttctggcaac taaaacaaaa ctcgttacgg ctaataaaat atccgtcagc gaggtcatag
     5581 gttcgccaat aattaaattg cctagtttaa gtgttgccag ttccaaaagc tgcaatgtat
     5641 tgtttggtcc cggaagaaat caaagcctca tccgggcaac aaagatacga aatcgcaatg
     5701 tggcagagaa atagtcctat tttaaagtta ggatccatgt ggcaatagcc tccatagcct
     5761 gctcgttaaa ggtttcctct atatggctgt attcagaagg agagcctgtg gcagattgtt
     5821 ggaacaggtg gttcagcccg tcgaacgcca tagtgctgta ttgcttattt tttccttctt
     5881 tcagcgctgc ttcaatggcg gaaagattct ctttataggg aacttgaaga tcatgcgtgc
     5941 cattgacggc aagaaccgga atttttaatt tgcgcagggt gggctgaggg tcataattca
     6001 gaaaataatg catccaggca ttagcataaa aatttactgc tgccagtgta tctgtctccg
     6061 tcatggacaa cagattcaga tattccgctg aagaattttt aatgaggttt ttattgtaag
     6121 caatgatagc tgatttaatc attgcaggat catcatttgc tttagcaatg tttatgatat
     6181 ctcttctata tttttcagtt gcctgaagcg catcttccgg tgcacccatt gcccttgcta
     6241 tcagatctgc ctggagattt aaaatggaat cgccgggtat acctgttcct gccagcaaca
     6301 ctatgaaatc tatctgttta tttttcgatg ctgccaaagg tgctatcacc ccaccttcac
     6361 tatgtccggc aataccaatc ttgccgatat tgagatcttt acgtgataat aaataatcaa
     6421 ccgcagcatt ggcatctgat gcaaagtctg ctgttgttgc tgtttcgtga tctccggtag
     6481 attgtgctac acctctatcg tcgtaacgca atacaataat gcccttgcgt gtgaaataat
     6541 cgcttaatac gagaaatggt ttatgtccca gcaaggcctc atttctatcc tgcggtcctg
     6601 aaccggtaac cagaataaca gcagtaaaag gccccttccc ttccggtatt gtgagtgtgc
     6661 cggccagtgt aacatcattg gtttgtttgt ttttgaatgt tacttcttct tctttataag
     6721 ggtatggttt aacaggctcc tgagggcgta caattccttt cacctctgtc accttagtca
     6781 gcgtcatcgg cattggcgca ccttgaataa atacgccgga aatagattgt gttgcagcag
     6841 tataaacacc tttaaattgt gctttgatca atggaatttc aacctgaatt tcatttgatc
     6901 ctgctgtata agttgttgag gatgtgggga tgtcgtttac tccctgatca ggactatcaa
     6961 gcgtggtgaa aagactgtcg tta
//
LOCUS       DAOQHY010000163         6881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_5838_length_6881_cov_5.268679, whole genome shotgun sequence.
ACCESSION   DAOQHY010000163 DAOQHY010000000
VERSION     DAOQHY010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5838_length_6881_cov_5.268679"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..422
                     /locus_tag="PLL28_11825"
     CDS             48..422
                     /locus_tag="PLL28_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70057.1"
                     /translation="MKRIVIIILTFAYLSGICSPFYTYVGYLVNKDFIATNLCENKDK
                     PEMNCEGKCQLTMQLAKDVEQKQDTKAPQSGNRELNPHTIASFNFTSYIGVISVFTEN
                     HSQQQFADMYIAAPESPPRLFV"
     gene            482..883
                     /locus_tag="PLL28_11830"
     CDS             482..883
                     /locus_tag="PLL28_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70058.1"
                     /translation="MKFNTAIVLACTIFAISATSCKEDEVPEVTYDVTFTIVEPEADF
                     VAVSGDELHMEVDLQGTKAIGNAEMLLIDEMSSDTLFTYSTTTTSDFLTMHEHYIPSV
                     TMSTMCHFVVSAWESNYADRLTEEVHLTINP"
     gene            886..1779
                     /locus_tag="PLL28_11835"
     CDS             886..1779
                     /locus_tag="PLL28_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70059.1"
                     /translation="MRRLFALAILLNLILMVKPLSACDQCGCSSGATFNNLSAFATYN
                     FIMLRLGYSSMQGYGNVGSFALNTNADIVGGYSLNKRLHLSAFLPVKYNYFEHDENSH
                     TASGIGDAGIMANYVLFSNSDKMMQRVTSTLSIRGGIELPTGNFNENFRADDVPAAIS
                     TGSGSFDFFGGLRYTIRSGNNAITVDYIGKYNTENAVAYQFGFQQSANVYVTKSFKKT
                     NAAWLPYAGVSGEFVSGDTYHDNFMTGTKGSNVSAMAGLEYARTKWVFGAHTEIPLIS
                     AYDTDINLHPKASLRMVHLFN"
     gene            complement(1850..3445)
                     /gene="rny"
                     /locus_tag="PLL28_11840"
     CDS             complement(1850..3445)
                     /gene="rny"
                     /locus_tag="PLL28_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNF70060.1"
                     /translation="MNETVMYVIIGAAALLLGILGGRLIFAPSKNQEALAKKEAEEII
                     ALAKKDAELITQKSKTEADTYKKERSLELKDQMIQQKAEVEKERQHKVGKLEEQELRL
                     KQRESQLGQQQSQVSKKEAELNNQRDTLNKQLESVNAKKAELDKAYETHIRKLEEVAK
                     LSADQARNELMEALKDEARNKAMAHIKEIVEEAKLKANKEAKKIILQTIQRTAAEQAI
                     ENSVSVFNLDSDDQKGQIIGREGRNIRALEAATGVEFIVDDTPETIIISGFDPVRREV
                     ARLSLQRLVTDGRIHPARIEEVVAKTKKQMEEHIVEIGERTVIELGVTGLHAELIRMV
                     GRMRFRSSYGQNLLQHSREVANLCAIMAAELGLNPKLAKRAGLLHDIGKVPEEESELS
                     HALLGAKLCEKYGEHAAIVNAVGAHHDEIEMQYVISPIVQACDAISGARPGARREILE
                     SYLKRIKEMEGLALGHDGVEKAYAIQAGRELRVIVESEKVDDKRAEELSFSIAQKIQD
                     EMQYPGHIKVTVIREKRAISFAK"
     gene            complement(3525..3836)
                     /locus_tag="PLL28_11845"
     CDS             complement(3525..3836)
                     /locus_tag="PLL28_11845"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNF70061.1"
                     /translation="MESEESKLLNIHVTICDRPYRLKVRPEEEETVRKAARTIAEKIK
                     ELQNQFAGSDKQDYLAMALLTAMVEQLGAHKQSISQASDDGLAVALDTLNQQLADALQ
                     Q"
     gene            complement(3925..4236)
                     /locus_tag="PLL28_11850"
     CDS             complement(3925..4236)
                     /locus_tag="PLL28_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70062.1"
                     /translation="MEQSDNSEKLQLLRSNVERLLRSFQAVQQENDILKQQIEAIRRG
                     SAEKTKRIEALEQQAELQKTAQTIANIARNEDGDDKTEVRKRINDLIKEVDNCIAMLS
                     E"
     gene            complement(4259..6700)
                     /gene="pheT"
                     /locus_tag="PLL28_11855"
     CDS             complement(4259..6700)
                     /gene="pheT"
                     /locus_tag="PLL28_11855"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNF70063.1"
                     /translation="MKISWNWLNELLPLPMSADEAAVLLTDIGLEVEGVYPYESIRGG
                     MQGLIVGKVVTCEKHPDADKLKVTTVDVGGGELLTIVCGAPNVAAGQKVIVAVVGTTV
                     YPVQGEPFAIKKAKIRGQESNGMLCAEDEIGLGESHAGLLILPEDTPIGIPVASLFDI
                     YTDQIIEIGLTANHADANSHFGTARELRAACIIRKGLDIAIQDLSLAQISVNKSAPKI
                     QVEVKNTDACPRYSGALLHNIKVESSPKWLQHKLRAVGMRPINNVVDITNYILHLYGQ
                     PLHAFDADKIKGNKVIVQTQPAGTTFVTLEGKSRSLKSEDLMICDAEEGMCIAGVYGG
                     LHSGITNESSSIFLESAYFNPSFIRRTESAHGLKTDASARFAKGTDIEITIKALSHAI
                     ALLQEICGATIEGDIIDIYPQVMSPFAVDLRAARLQAISAIQIEDSIVENILGALNIT
                     IARKSTGVWHTLVPTYKNDVTREIDLIEEILRMYGFNNIPTPKTVRTPYLVSPKPDRE
                     KVKMDTIRHLVSQGSYEIATNAISRSKYNQQFLPQLMHLQPTLLNSLNAELDSMRITM
                     AFTGLEVIAYNLSHKQSDLNLFEFGRVYHKTADAYREPEVLAFYKTGNVFAESWRIQQ
                     RKTGFYDLKEQVEKLMLHMGVKASWKPVDETHPLRPLFEQAVTVSVGNEVIGICGGIH
                     NAVLQSFGIKQAVWFAEINWQPLLLAMASRKVIFKEINKYPEMRRDLALVLDADVEYQ
                     SIAAIARQEGKQILREVNLFDVFEGEQLAGRKSYAIGLTFNDSTRTLTDSEVDAVMQR
                     LISRYEKEFNAVIRK"
BASE COUNT         1800 a         1544 c         1372 g         2165 t
ORIGIN      
        1 cataaacctg aataggtgag tatgtatctt tgcactacta ccctgaggtg aaacgtattg
       61 ttatcatcat actgaccttt gcatacctgt cgggcatctg ctcgcctttt tatacgtatg
      121 taggttacct cgtcaacaaa gactttattg caacaaacct ttgtgaaaac aaggataagc
      181 ctgaaatgaa ctgcgaagga aagtgccagc ttactatgca attggcaaag gatgtggagc
      241 agaaacagga tacaaaagcg cctcaatcag gtaaccgtga gttaaatcct cacaccatcg
      301 catcgtttaa tttcacttct tatataggcg taatttctgt ctttacggaa aatcattcgc
      361 aacaacaatt tgccgatatg tatattgccg caccggagtc ccctccgcgt ttgtttgtgt
      421 aattacaacc ctgtgcgttg gcacttgaat cttttttttt aattacacaa actaaatttt
      481 tatgaaattt aatactgcta ttgttttagc ctgtaccata ttcgctattt ctgctacctc
      541 atgcaaagaa gatgaagttc ctgaagttac ttatgatgta actttcacca ttgttgaacc
      601 ggaagccgat tttgttgccg tgtcgggtga tgaattgcac atggaagttg acttgcaagg
      661 aacaaaggcg attggtaatg ccgagatgtt gctcatagat gaaatgtcaa gcgatacttt
      721 gtttacttat tcaaccacca ctacctcaga tttcctgacc atgcatgaac attatattcc
      781 gtctgttacc atgtctacta tgtgtcactt tgtggtttct gcatgggaaa gtaattatgc
      841 cgacaggtta actgaagaag tgcatctcac tatcaatcca tgattatgag acgccttttc
      901 gcattggcta tattattgaa tctgatatta atggtgaaac cactgtctgc atgcgatcag
      961 tgcggatgca gcagtggcgc cacttttaat aacctcagtg cattcgccac ttacaatttt
     1021 attatgctaa gacttggcta tagcagtatg caaggttatg gtaatgtagg ctcctttgca
     1081 ttgaatacca atgccgatat tgttggtggc tatagcttga ataagcgatt gcatctgagt
     1141 gcttttctgc ctgtgaagta caattatttc gaacatgacg aaaatagtca tacagcttca
     1201 ggaattggtg atgcgggtat tatggctaat tatgtgttgt tttcgaatag cgataagatg
     1261 atgcaaagag ttacatcaac actgagcata cgcggaggga tagaacttcc aacaggaaat
     1321 tttaatgaaa actttcgcgc tgatgatgtt cctgctgcta tttccaccgg ctccggaagt
     1381 tttgattttt ttggcggatt gcgctatact atcagaagtg gcaataatgc catcaccgtc
     1441 gactatatcg gtaaatacaa tactgaaaat gcagttgcct atcaatttgg atttcaacaa
     1501 tcagccaatg tatatgtaac gaaatcgttt aaaaaaacaa atgcagcctg gttgccttat
     1561 gccggtgttt caggcgagtt tgtgagcggc gatacttatc acgataattt tatgacaggc
     1621 accaaaggaa gtaatgtttc agcaatggcc ggcttggaat atgcacgtac gaaatgggta
     1681 tttggtgctc atactgaaat tccgctgatc tctgcttatg atactgatat caatctgcat
     1741 cctaaggcct ctttgcgcat ggtgcacctg tttaattgag gtttaatgtg atccataaaa
     1801 aagggctgtt gagaaatcaa caaccctttt ttttatgaaa ataccatgct tatttggcga
     1861 aggaaatcgc ccgtttttcg cgaataacag taaccttgat atgtccggga tactgcattt
     1921 catcctgtat tttttgcgca atgctgaacg acaattcttc agcgcgctta tcatccactt
     1981 tttcagattc tacgatcacg cgtagctcac gtcccgcttg gatagcatag gctttttcaa
     2041 ccccatcatg accaagtgca aggccttcca tttctttaat gcgcttgagg tagctttcca
     2101 gaatctcgcg acgggctccc ggtcttgcac cgctgatagc atcgcaagcc tgaacaatcg
     2161 gagagatcac atattgcatt tctatctcgt cgtggtgtgc accaactgca ttcacaatag
     2221 cagcatgttc accatatttt tcgcacagct tggcacctaa taaagcgtga ctcaattcag
     2281 attcttcttc aggcacttta cctatgtcgt gtaacaaacc tgcgcgtttt gccagtttag
     2341 gattgagtcc caattctgca gccatgatag cgcagagatt tgcaacctcg cgagagtgtt
     2401 gtaataagtt ctgaccataa gaagaacgga aacgcatacg tcctaccatt cgaattaatt
     2461 cagcatgtaa accggtaaca cccaattcaa taactgtgcg ctcaccaatt tcaacaatat
     2521 gttcctccat ctgctttttc gtcttcgcaa caacttcctc gatacgagca gggtgaatac
     2581 gaccatcagt taccagacgc tgtaaactca atcgtgccac ttcgcgacga acagggtcaa
     2641 atccgctgat aataatagtt tcaggcgtat cgtcaacaat aaattctaca ccggttgcag
     2701 cttccagcgc acgaatattt cgaccttcgc gaccgatgat ttgtcctttt tgatcatcgc
     2761 tgtcgaggtt gaaaactgaa acggaatttt caattgcctg ctctgcagca gtgcgctgta
     2821 ttgtttgaag aatgattttt ttagcttctt tatttgcctt caatttagcc tcttcaacaa
     2881 tttctttgat atgcgccatc gccttattac gggcttcgtc tttcagcgct tccattaatt
     2941 cgttgcgcgc ctggtcggca gataatttgg ctacttcttc gagttttcgg atatgcgttt
     3001 cataagcttt gtcgagctca gctttttttg catttactga ttctaactgc ttatttaaag
     3061 tgtcgcgttg attatttaat tcagcttctt ttttagaaac ctgcgattgc tgttgtccta
     3121 attgcgactc tctttgtttg agccttaatt cttgttcttc gagtttacca accttgtgtt
     3181 gtctttcttt ttcaacctcc gctttttgct ggatcatctg atccttcagt tctaaagagc
     3241 gttctttttt ataagtgtcg gcttcagttt tgcttttctg agtgatcagt tctgcatctt
     3301 ttttagcgag ggcgataatt tcttcagctt ctttttttgc cagtgcttcc tgattttttg
     3361 agggtgcgaa gatcaagcga cctcctaaaa tgcccagtag tagggctgcg gcaccaataa
     3421 tgacatacat tactgtttcg ttcatagcta tgattttatg atgacgaacg taggccgtga
     3481 ccagcaaaat acatctaaaa aggagttaca ggtagggagt atgatcactg ctgcaaagca
     3541 tcagcgagtt gttggtttaa ggtatcaagg gcaacggcta aaccatcgtc cgaagcctgt
     3601 gatatgcttt gtttatgtgc tcccaattgc tccaccattg ctgttaacaa cgccatagcc
     3661 agataatcct gcttgtcgct gcctgcaaat tggttctgga gttcctttat tttttcggca
     3721 atggttcggg ccgcctttcg cacagtttcc tcttcttccg gccggacctt taacctgtaa
     3781 ggcctgtcgc aaatcgtaac atgtatattc aacaacttgc tttcctcgct ttccatctga
     3841 ttttgttgca aacggtttag tccaatacgg actaaagtta agtaaatctt atgataagtg
     3901 tcactacagc taaataagga aaccttattc gctcaacatg gcaatgcaat tgtccacttc
     3961 tttgatcaga tcattgatgc gcttgcgtac ctcggttttg tcgtcaccat cctcgtttcg
     4021 ggcaatgttg gcaatggttt gcgctgtttt ttgaagctct gcctgctgtt ctaacgcctc
     4081 tatccgcttg gttttttcgg cactgccacg gcgaatagcc tcgatctgct gcttgagaat
     4141 gtcgttttcc tgctgaacag cctgaaaact gcgcaacagc cgctcgacat ttgacctgag
     4201 caattgaagt ttttctgaat tgtcgctctg ctccatttaa aagctaatct acaacttttt
     4261 acttgcgtat caccgcatta aattcttttt catagcgaga tatcagcctt tgcattacgg
     4321 catctacctc tgaatcggtt agggttcgtg tgctgtcatt aaaagtcagt ccaatcgcat
     4381 agctctttct gcctgctagt tgctctcctt caaatacatc gaaaaggttt acctccctga
     4441 gtatctgctt gccctcctga cgtgctattg ctgcaatgga ctgatattct acatcagcat
     4501 ccaataccaa tgccaggtcg cgacgcatct caggatattt attaatttcc ttgaaaatca
     4561 ctttcctcga tgccatagca agcaataagg gctgccaatt tatctctgca aaccataccg
     4621 cctgcttaat gccgaaggat tgtaaaacag cattgtgtat acctccgcat ataccaataa
     4681 cttcatttcc cacagaaaca gttactgcct gctcaaataa tggacgcaac ggatgtgttt
     4741 cgtctacagg tttccagctt gctttcacac ccatgtgtaa catcaatttc tcaacctgct
     4801 cctttaaatc atagaaaccg gttttgcgtt gttgtatccg ccagctttca gcaaatacat
     4861 ttccggtttt gtaaaatgcc aatacctccg gctcgcgata agcatcggca gttttgtgat
     4921 acacacgtcc gaattcaaat aaatttaaat cgctttgctt gtgactgaga ttataggcta
     4981 ttacttccag tccggtgaat gccatggtaa tgcgcatgct atcgagttcg gcattcaatg
     5041 aattcaacag ggtaggttgc aaatgcatta attgcggaag gaattgctga ttgtatttcg
     5101 aacgcgaaat ggcattcgtt gcaatctcat acgagccctg agaaacgagg tgacgaatgg
     5161 tatccatttt cactttttcc ctatcaggtt tcggactcac caaatatggc gtgcgaacag
     5221 tttttggtgt ggggatatta ttaaaaccat acatcctcaa tatctcttcg ataagatcaa
     5281 tttctcgtgt aacatcattt ttatatgtag gcaccaatgt atgccaaaca cccgttgatt
     5341 tacgcgcgat ggtaatattc aatgcaccta aaatattttc aacaatgctg tcttcaattt
     5401 gtatggcact gatagcctgc aatcgagctg cacgcagatc aacagcgaag ggcgacatga
     5461 cctgaggata tatatcaatg atatcaccct cgattgttgc accacatatt tcctgcaaca
     5521 atgcaattgc atgcgataag gcctttattg tgatttcaat atctgtaccc tttgcaaaac
     5581 gtgccgaagc atcagttttt aatccgtgtg ctgattctgt tctgcgaata aatgaaggat
     5641 tgaaatatgc gctttctaaa aatatagagc tgctttcatt tgtaatacct gaatgcaatc
     5701 caccgtatac tccggcaata cacatgcctt cctcagcatc gcaaatcata agatcttcag
     5761 acttcaggct gcgcgatttc ccttccagag ttacaaatgt tgttccggca ggctgtgttt
     5821 gtactattac tttattgcct tttattttgt ccgcatcaaa tgcatgtaag ggctgaccat
     5881 ataaatgcaa tatgtaattg gtgatgtcaa ctacattatt aatcggacgc atgccaacag
     5941 cgcgcaattt atgttgcagc catttgggtg atgattcaac tttaatatta tgtaacaatg
     6001 cacctgagta gcgcggacat gcatcagtat tttttacttc aacctgtatt ttcggagcgc
     6061 ttttgttcac tgaaatttga gcaagggaca gatcctgaat ggcaatatcc aatcccttgc
     6121 gaataatgca ggcagcgcgc aattcacgag ccgttccaaa atgactattg gcatcggcat
     6181 gatttgctgt gagtccgatt tctataattt gatcggtata aatatcgaat aaagaagcaa
     6241 ccggaattcc gataggtgta tcctcaggca gtatcaataa tccggcatgt gattcaccta
     6301 atcctatttc atcttcggca cacaacatgc cattgctctc ctggccacga atttttgctt
     6361 tcttaatggc aaatggttct ccttgaacag gataaaccgt agttccgact acggcaacta
     6421 tcaccttttg tccggcagca acatttggcg caccgcacac gatggtgagc aattcaccac
     6481 cgccaacgtc tacagtagtg accttcaatt tgtcggcgtc gggatgtttt tcacaggtaa
     6541 caactttgcc cactatcaat ccctgcatgc caccgcgaat gctttcgtag ggataaacac
     6601 cttcaacttc cagtccgata tcagtcagca aaacggcagc ttcatctgca ctcataggca
     6661 gtggaagcaa ctcattcagc caattccatg aaattttcat actctgcaaa gttaataacc
     6721 ctgcgcaatt ttggtggttc tgagggatta aaggggtttg gctttcgaac tccatgagga
     6781 tatggaatca ggtatcaggt atcaggtatc aagtatcagg tatcaagtat caggtatcaa
     6841 gtatcaggta tcaggtatca agtatcaggt atcaagtatc a
//
LOCUS       DAOQHY010000164         6841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_5892_length_6841_cov_4.110374, whole genome shotgun sequence.
ACCESSION   DAOQHY010000164 DAOQHY010000000
VERSION     DAOQHY010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5892_length_6841_cov_4.110374"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="PLL28_11860"
     CDS             <1..744
                     /locus_tag="PLL28_11860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70064.1"
                     /translation="SEDGEIPIWLNTAGITLTPGYNEIPIMLGSFDVQAEEIYGLTFH
                     IACSGVTEAIVDSIDVQVKFDDSFLGTTDNLVGINHPYDTPDFGIDAGVVGIDHINKS
                     GYGQIGRLSFVVEDNIAGILVGSGDSTLYFFISDVTGVTAEMSEVSIYGNVFPVMVNG
                     INNQKEPQLLSVFPNPVIGSEIFIKGLPPGPNDFSYDILNTSGQVVATGKVTQDKIIN
                     NTLIQLAQGFYTLKVTGAYGVFYSTIVID"
     gene            777..1727
                     /locus_tag="PLL28_11865"
     CDS             777..1727
                     /locus_tag="PLL28_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70065.1"
                     /translation="MKSAFIFCISLLALSFWTSCADEHVTLNGNLYNSLSEVYAELAP
                     PVQTFTINADIGGTVTGLKGTSIEFPADAFIDQQGAIVSGNVTVTIIEVLTESEMIGS
                     GIYPINTNGDLLSSGGEIYINVSQDGERLTMTPGAYYILQVPAFNDQSMGVYEGIGAT
                     ADSFWNYVSPTLDSLPFGGTYRDTAIYSLLEDVYTVISDILGWVNCDAFPDVQFINCN
                     VHYTGVDDLDAGLTSVIFIADYYMLSAYPSPFVYDTPSNTSTECYLPVTSGYILTTTI
                     KAGVLYFGITTDITPIEGATYEITLTAGTPDDLAALLDAL"
     gene            1900..2316
                     /locus_tag="PLL28_11870"
     CDS             1900..2316
                     /locus_tag="PLL28_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70066.1"
                     /translation="MQTHTRKTILPVFFTIGAAILFSCNDKDPTADDKIGPDITVALP
                     DDNTVYAPGDTIYYQADIEDDSELSEMSVSLINGTDTVLLWPDATVNYGNIKSYTINN
                     WDIDIWNVNADATIQFKAVDKHDNSSTADVPVQLTM"
     gene            complement(2317..3552)
                     /locus_tag="PLL28_11875"
     CDS             complement(2317..3552)
                     /locus_tag="PLL28_11875"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNF70067.1"
                     /translation="MSIDFSAFDFAPALSEGLDAMGFRNPTPIQEQAIPFILSGNDLI
                     ACAQTGTGKTAAFLLPVMHKIISGNSDGVNTLIIVPTRELANQIDEAIRGIAYFTSLS
                     SIAVYGGNDGIMFEQEKRAFREGVDIIVGTPGRLLSHIQQGYLKFDTLKHLILDEADR
                     MLDMGFSDDIAKIISNIPHHPQTLLFSATMPHKIREMAKRILRDPQQINIALSKPAAG
                     VSQGVYIVHDAQKMPLLVDILSQKQLKSMLVFASTKDKVKQLELELKRRKFNVAAIHS
                     DLEQAVRKSVLRSYANREIQILVATDILSRGIDVDEIGLVINYDVPGDAEDYVHRVGR
                     TARAESTGEAITFVGPADQSKFSRIEQLIGDTIPRLEIPESLGPGPEYNPRAHHGHAK
                     SGGRPNQRRRRPNGGNRRH"
     gene            3633..5120
                     /locus_tag="PLL28_11880"
     CDS             3633..5120
                     /locus_tag="PLL28_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70068.1"
                     /translation="MKTIQLLLACMLFTHGVFAQVTTEKSIEFEEIEGYDGFSIEPFG
                     KNGLLVYSKSEEGNSYRIQRYNTDLEPEEKKDITLPKGYFMIGEYLDDESINFLFSNK
                     KGQFIFYQVNGNTFDLSKASGIFPKKTLPGFFVAKGNFAYITRSNKKGVTVIQLNIST
                     GKSKIIPLNISPYTLKDLTVEKIQPIAESNETFIYVNAYNKKDHNLYVMQVNESGTVT
                     NTFNLSKDTEKKLSSVSASPIGDNEYVFTGTYSSRSSATSEGMYICSATNGKVNFMKF
                     YNFVDFTEFFSYLPERTQEKIEKKKAKADDKGKEFSYNYYIAEHPIRKIGDNYLLVGE
                     AYYPTYRQEAYTSYVNGKPVTNYRTVFDGYQYTHATIAMFDSEGGKLWDQTFKMYPGT
                     KPFTVKRFISETYNDDELKMVFASLNSIKSMSVGYDGKISNERSAEIIKVGNDDEKVK
                     SAYSNIEYWYGNTFVAYGNQLLKNSKESIGNKKRRVYYINKIVFE"
     gene            complement(5175..5570)
                     /locus_tag="PLL28_11885"
     CDS             complement(5175..5570)
                     /locus_tag="PLL28_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNF70069.1"
                     /translation="MYYAQTCAAKGTSGNNTRQLLYRSMERAAVNIYRTENSYEMLVF
                     APGRIREHFDVHMEGSELTVSYRPPEGFPRPEWVRREYSRGGFVRQFTLGAGIDTAGI
                     AATYVNGVLEIHLPFLKGEENAKHKIEVK"
     gene            complement(5745..6137)
                     /locus_tag="PLL28_11890"
     CDS             complement(5745..6137)
                     /locus_tag="PLL28_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70070.1"
                     /translation="MQKLRTTILIAGIILVSSSAFAAIQPDGTSLMHAWNIQSVELFG
                     IATAPDETMAGDMLDLNADMTFTAIVDGNALSGTYGIDQSNTWITLYISADNAMRIKV
                     IESDATHIKTTRVDGDGNQTIVTYTIAD"
     gene            6250..>6841
                     /locus_tag="PLL28_11895"
     CDS             6250..>6841
                     /locus_tag="PLL28_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70071.1"
                     /translation="MQRLLPICILLSSSLNLQAQSLVTALIQHGNDSQQTTSIVADPD
                     NYMVAAVNFWDDLDADPGVPVVNFNTAGTMDVAIIKLDPAGNYVWGKQLAGAGWNASS
                     DIACDALGNIFIFGYYNGTMDMDPGPGTSNITSAGGDDIFVGKYDTNGNLLWAYSIGG
                     TGTEQNYGFDVGPDNNTVLYGYFQNTVDFNPGGGTFN"
BASE COUNT         2063 a         1399 c         1369 g         2010 t
ORIGIN      
        1 agcgaggatg gtgaaattcc aatatggctt aataccgccg gcattacact aacccccgga
       61 tacaatgaaa taccaatcat gcttggatct tttgatgtgc aagcagaaga aatatacggt
      121 ctgacatttc atattgcttg cagtggtgtt actgaggcaa tagttgattc aatagatgta
      181 caagttaaat tcgatgattc atttcttggc actaccgata accttgtcgg tataaatcac
      241 ccctacgaca cgcctgattt tgggatcgat gccggtgttg ttggcataga tcacatcaat
      301 aaatcagggt acggtcagat tggaagatta tcgtttgtgg ttgaagataa tattgccggt
      361 attttagtcg gctccggcga ttcaacatta tacttcttta tttcagatgt cacaggagtt
      421 acagctgaaa tgtcggaggt ttcaatttat gggaatgttt ttcctgttat ggttaatggg
      481 ataaacaatc aaaaggagcc acaactgctc agtgtatttc cgaatccggt tattggaagt
      541 gaaattttta tcaaaggttt acctccggga ccaaacgatt tcagttatga tattctaaac
      601 acctcgggac aggtagttgc gacaggcaag gttactcaag ataaaataat caacaacaca
      661 ttaatccaac tggcacaggg cttttacacg cttaaggtaa ccggggcata tggtgtattt
      721 tattcaacaa tagttataga ttaatcacaa tttaattcat tattaaacta attaccatga
      781 aatctgcttt catcttttgc atctccttac ttgctctttc attttggaca agttgcgccg
      841 atgagcatgt aaccttaaat ggaaatttat acaattcatt atcagaggtg tatgctgaac
      901 tggcacctcc tgttcagaca tttacgatta acgccgatat tggtggaaca gttaccggat
      961 taaaaggaac tagcattgag tttcctgctg atgcatttat cgatcaacaa ggcgctattg
     1021 tcagcggtaa tgtcaccgtt actatcattg aggttctcac cgaatctgaa atgatcggct
     1081 ccggtattta cccgattaat acaaatggcg atctgttgag ctccggtggc gaaatttata
     1141 tcaatgtatc acaagatggc gagcggctca ccatgacacc cggagcatac tacatattac
     1201 aagttcctgc cttcaatgat caatccatgg gtgtttatga aggtattgga gctacagcgg
     1261 atagtttttg gaattatgta tcacccacgc ttgattcact tccttttgga ggaacttata
     1321 gagacactgc aatatattct cttctcgaag atgtatatac tgtaatttca gatatacttg
     1381 gttgggtgaa ttgcgatgca tttcctgatg tacaatttat aaactgcaat gtgcattata
     1441 cgggagtcga tgatcttgat gccgggttga caagcgtaat ttttattgca gattattata
     1501 tgctctccgc ctacccgtct ccatttgttt atgatacccc gtcaaacacc tcaacagaat
     1561 gctatttacc tgtcacctcc ggttacattc tgactacaac aattaaagcc ggtgttttgt
     1621 atttcggaat cacaactgat atcacgccta ttgaaggtgc tacatacgag ataacactta
     1681 ccgccggaac gcccgatgat ctggcggctc ttctagatgc attataaaac aaacgttaac
     1741 actactccta tgtggtggca ctaaggattc ggttttagct ttccacatga aaacggcagt
     1801 atccaactgc agatccggtc ccgggcttaa taaagtccgg gatctttttt tgtttatata
     1861 ttaaaatatt cccttacctt agtattcaac acaagcaata tgcaaacgca cactcgcaaa
     1921 acaattttgc ctgtattttt tactataggc gccgccattc tgttttcctg caacgacaaa
     1981 gaccctaccg ccgatgataa aatcgggccg gacattactg ttgctttgcc tgatgacaat
     2041 accgtatacg cccccggaga taccatttat taccaggcag atattgaaga tgattcggaa
     2101 ttatctgaaa tgtcggtatc tctcatcaat gggactgaca ccgttctact ttggcctgat
     2161 gctactgtga actacggtaa tatcaaatcc tatactatca ataattggga tatagatatc
     2221 tggaatgtga atgctgatgc aactattcag tttaaagctg ttgacaagca cgacaattcc
     2281 agcactgcag acgtacctgt tcagcttacg atgtaattag tgccttcgat taccaccatt
     2341 tggtcggcga cgcctttgat tcggtctgcc gcctgatttg gcatgtccat ggtgtgcacg
     2401 tgggttatat tcaggtccgg gacctaaaga ttcagggatt tccaagcgag gaatggtgtc
     2461 accaatgagt tgttctatgc gcgaaaattt tgattgatct gctggaccca caaaagtaat
     2521 ggcttcaccg gtgctctctg ctctggcagt tctgcccacc ctgtgcacgt aatcttcagc
     2581 atcaccggga acatcataat taattaccaa tcctatttca tcaacatcaa ttccgcgcga
     2641 caaaatatcg gtggctacca aaatttgaat ttctctattg gcatagctac gtaatacact
     2701 ttttctaaca gcctgctcca ggtcggagtg tatcgcagct acattaaatt tcctgcgttt
     2761 taattcaagt tcaagttgct ttactttatc ttttgttgaa gcaaatacca acattgactt
     2821 caattgcttt tgcgataaaa tatctacaag caggggcatt ttttgtgcat catgcactat
     2881 atatacgcct tgagatacac cggctgccgg tttagaaagg gcaatattga tttgttgagg
     2941 atcgcgcaaa atgcgtttcg ccatttcacg aatcttatgt ggcatggtgg cagaaaacaa
     3001 taatgtttgc ggatgatgcg ggatattgga aatgattttt gcgatatcat cggaaaatcc
     3061 catgtccaac atgcgatctg cttcatccag aatcaaatgt ttcagtgtat caaacttcag
     3121 gtatccctgt tggatatgtg atagcaacct accgggtgtt ccaacaataa tatcgacacc
     3181 ctcgcgaaat gcgcgttttt cctgctcgaa cataatgcca tcatttccgc catatacagc
     3241 tattgaactg agagaggtga aataggctat acctctgatg gcttcatcta tctggttggc
     3301 aagttctctg gttggaacga tgatcagcgt attcacacca tctgaatttc cactgatgat
     3361 cttatgcatg acaggcaata aaaatgcagc cgtttttccg gtgccggttt gtgcgcatgc
     3421 aataaggtca ttaccgctta aaatgaaggg tattgcctgc tcctggatag gagtaggatt
     3481 gcgaaacccc atggcgtcta aaccttcaga aagagcggga gcgaaatcaa aagcagaaaa
     3541 atcaatactc acaataatca aagatacgga attatcattt gcctactaaa cgcttatagg
     3601 tatatttgaa gtaaatttca aacagccaca ttatgaaaac aatacaatta ttacttgcat
     3661 gcatgctttt tacacacggt gtttttgcac aagtgactac agaaaaaagt attgaatttg
     3721 aagaaataga aggctacgat ggtttttcca tagaaccatt tggcaaaaac ggtttactgg
     3781 tttatagcaa atcagaggaa ggaaatagtt atcggataca acgatataac accgacctgg
     3841 aaccggaaga aaagaaggat atcactttac ccaagggata ttttatgatc ggcgaatacc
     3901 tggatgacga atcgatcaat tttttgtttt caaataaaaa agggcaattc attttttatc
     3961 aggtgaatgg taatacattc gacctgtcaa aagcttcagg cattttccct aaaaaaacac
     4021 tgcccggatt ttttgttgcg aaggggaatt ttgcctatat cacccgaagc aataagaaag
     4081 gtgttaccgt tatacaatta aacatcagca ccggtaaaag caaaatcata ccattgaata
     4141 tttcgccata tactttaaaa gacctgaccg ttgaaaaaat tcagccgatt gcagaatcga
     4201 atgaaacctt tatttacgta aatgcttaca ataaaaagga ccacaacctt tatgtaatgc
     4261 aagtgaatga atccggcacg gtgacaaaca cattcaattt atctaaagac accgagaaaa
     4321 aattatcatc cgtgtcagct agtcccattg gcgataatga atatgtgttc accggcacct
     4381 attcttcacg tagttccgca acctctgaag gcatgtatat ctgcagtgca actaatggca
     4441 aggtaaactt tatgaagttt tacaattttg tcgattttac cgaattcttc tcttatctgc
     4501 ctgaacgcac ccaggaaaaa atcgaaaaga aaaaagccaa ggccgatgac aaaggtaaag
     4561 agttcagcta caactattac atcgccgagc atccgatcag aaaaatcgga gacaactatc
     4621 tgttagtagg agaagcgtat tatccgacat acagacagga agcgtatacc tcttacgtga
     4681 atggaaagcc ggtaactaat tatcgtactg tgtttgatgg ttaccaatat actcatgcaa
     4741 caattgccat gtttgattca gaaggtggta aattgtggga tcaaactttt aaaatgtatc
     4801 ccggaaccaa accttttacc gtaaaaagat ttatctctga aacatacaat gacgacgaac
     4861 ttaaaatggt gtttgccagc ttaaattcaa tcaagtcgat gagcgttgga tatgacggaa
     4921 aaatctccaa tgaacgctct gctgagatta ttaaagttgg caacgacgat gaaaaagtga
     4981 aatcagcata ttctaatatt gaatattggt atggcaacac cttcgtggcc tatggcaatc
     5041 agttgttaaa aaacagcaaa gaatctatcg gcaataaaaa gcgtcgtgtg tattatatca
     5101 ataagattgt gtttgagtga ttgaatgtta agaaatataa aaggctgacg catcacacgt
     5161 cagccttttg ttttttactt cacttcaatt ttgtgtttgg cattttcttc acctttcaaa
     5221 aaaggcaaat gaatttccaa aacaccattt acataagtgg cagcaattcc tgcagtgtcg
     5281 atgccggcac caagggtgaa ctgtcttaca aatccacctc tactgtattc ccttcgtacc
     5341 cattcagggc gcggaaatcc ttcaggtggg cggtaagaaa cggtaagttc gctgccttcc
     5401 atgtgcacat cgaaatgttc gcgaattcgt ccgggtgcaa acaccagcat ttcgtaactg
     5461 ttttcggtgc ggtaaatgtt tactgcggcg cgttccattg atcggtacaa taactgccgc
     5521 gtgttattgc cggaggtgcc ttttgcggca caggtttgtg cgtaatacat ctgattacat
     5581 tttttagtga aaaaataatt atccactaaa cagacgagca cagctaaatt acattgtatg
     5641 cacaaaagtt aaaattgcac aacatcatat tgcataaacg caataaggga gcaataacaa
     5701 aaaaaagaaa attaagttcc caaatgagct aagaccacat acactcaatc tgcaatggtg
     5761 taggtaacga tggtttgatt gccatcacca tctactcttg tagtcttaat gtgcgtagcg
     5821 tcagattcaa tcaccttaat tcgcatggca ttatctgcgc tgatatataa tgtgatccat
     5881 gtattgcttt gatcaatgcc ataagtacct gataatgcat taccatccac aatggcggta
     5941 aaagtcatat cggcatttaa atccaacata tctccggcca ttgtttcatc cggtgctgta
     6001 gcaatgccaa ataattctac agactgaata ttccatgcat gcatcaatga agtaccatca
     6061 ggttggatgg ctgcaaaggc agaagatgaa actaaaataa tacctgcaat tagaatagtg
     6121 gtacgaagct tttgcatata taatttttat aatatgttaa tcagatctag tataaaggta
     6181 atatatttat ataaatcacc ttggcacaat tcttggctac caaactcgtc aacacacaaa
     6241 aacctgccta tgcaacgatt attacccatt tgtattctgc tatcctcatc ccttaattta
     6301 caagctcaaa gccttgttac agcgctgatt cagcatggta acgattcgca acaaacgaca
     6361 tccattgtag ccgatcccga caattatatg gttgcggcag ttaatttctg ggacgacctg
     6421 gatgcagatc ccggcgtgcc ggtggtaaat ttcaatactg ccggcaccat ggatgtggcc
     6481 attattaaat tagaccctgc gggcaattat gtctggggaa aacaactggc aggtgcaggt
     6541 tggaatgcct cgtcagatat tgcctgcgat gccttgggaa atattttcat tttcggttat
     6601 tacaatggca ccatggatat ggatcccgga ccgggaacat cgaacattac atctgccggc
     6661 ggtgacgata tttttgtggg gaaatatgat acaaatggaa atttactttg ggcttattcc
     6721 atcggtggaa ccggcacgga acagaactat ggattcgatg ttggtccaga taacaacact
     6781 gttttatatg gttattttca aaataccgta gattttaatc ccggtggcgg aacgtttaat
     6841 c
//
LOCUS       DAOQHY010000165         6826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_5908_length_6826_cov_5.686900, whole genome shotgun sequence.
ACCESSION   DAOQHY010000165 DAOQHY010000000
VERSION     DAOQHY010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5908_length_6826_cov_5.686900"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /locus_tag="PLL28_11900"
     CDS             <1..1418
                     /locus_tag="PLL28_11900"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HNF70072.1"
                     /translation="ITSQIPLMASQQRHVQHLYLRAGFGESPAIVKQRSAKEIKQLVD
                     DLFTESKQYRNLTYLENPAKGKEVSNLKVLLLILKSQEQMRELNNVWMGMLASTKAVL
                     REKMTLFWHDHFATSTQFAYLMQLQNNTLRKHALGRFDKLLHAIAKDPAMIIYLNNQQ
                     NKKDAPNENFAREVMELFTLGEGNKYTEADIKEAARAFTGWTVNKKGEFEFVAKDHDD
                     ESKTFLGRAGNFNGEDIINILLEEKQTAKYVTTKIYKEFVNDNVNDAHVEALSTIFFD
                     SGYDISALMRNIFTADWFYADENMGSLIASPVDLITRFRRLIKMEFKEEKGQFNTQKI
                     LGQVLFFPPNVAGWKGGRNWVDSSTLMLRMQIPTRIIRGGGFNVRPKPEFEDAPEDEM
                     TLKTSKKADVRSDWSGVVNYFKDVPTDQLTESVIETFIQSSSDHIDRNIIAQYVDNSS
                     DEKRILSTIGVVMSLPEFQLI"
     gene            1420..2625
                     /locus_tag="PLL28_11905"
     CDS             1420..2625
                     /locus_tag="PLL28_11905"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNF70073.1"
                     /translation="MAPNRRDFLKQTGLASAGILVPSFIKASVPFLPGDVRPKVLVMI
                     QFTGGNDGLNCIVPIGNDLYHKARPNIGYKKEELIKLSDDIGMNANLQGLADLFYEGN
                     VVLINNVGYPDPNRSHFRSMDIWQTASDENVYMQTGWIGRMLDQSCKSGDCQKPHFAV
                     EIDDSLSLALKGESMSGFAVRNPDQLKNVNLNPFIDSVARSGGDIHTHDNVGYLHKLL
                     TDTTESTDYIYDQSKKYTTAFTYPTSEFAAQLKIIANLINAETNTSVYYVSLSGFDTH
                     ALQRGQQERQLRIYSEALQAFTKDLKSTNRFKDTLIMTFSEFGRRVEENAGKGTDHGT
                     ANNVYLIGGGMKQGGMINDMPDLSKLVDDDLVYQTDFRNIYATILDKWLEVPSKEILG
                     RDFARMDFL"
     gene            complement(3190..4731)
                     /locus_tag="PLL28_11910"
     CDS             complement(3190..4731)
                     /locus_tag="PLL28_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70074.1"
                     /translation="MKNLIEISKIVTKKRISKIEIFDKSLLNKRDSKFNEFYEGLVND
                     RFTTDEEAASILYETSPLDDKYRQLKSRFSKRLLNTLFFLDSNDPSFSDYHRANYSCN
                     KNWAQIRILLSSGARSSAIKIATKTLSVAQTYRITDMILNCARILMSNASLSGDDNEY
                     DYYADLTHKALRDMGFEIRSEELYQQLSINFAAAGYSDPELKALADKSLDEVNKLIKQ
                     TDSYQLHYMRYQIWIIGTQIKGQFKETIRVCEEAENYLESNPNFNQKIKLANLALIKI
                     SAYLQLRDYDNGKRNAEKCLELVQEGSNNWFVFLEYYFLLAMHTENYINAAAIFIKVI
                     NHPRFMYTGDERKEKWKIFEAYINYIFETENLDRDLLATDATKRKFRLLKFLNEVPNF
                     SKDKRGYNVSILVIQILYMLEKGEYSGIINRSEALNVYCSRYLRKDDAYRSSCFLKML
                     LTMVKEDFVFDRTKQMAQKYLRKMMAGDPSGNNIVNEWEIIPYEILWEKVLNRLKNKT
                     VAKQV"
     gene            complement(5345..6580)
                     /gene="clpX"
                     /locus_tag="PLL28_11915"
     CDS             complement(5345..6580)
                     /gene="clpX"
                     /locus_tag="PLL28_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNF70075.1"
                     /translation="MKKKDSAYCSFCLRPKEDVRILIAGQEGHICENCVAQAQQMVDE
                     ELMQPVKRYKFALTNSMKPRSIKEFLDTYVIGQDEAKKILSVAVYNHYKRLNQKPLED
                     VEIEKSNIMVVGRTGTGKTLLARSIAKFLNVPFTIVDATVFTEAGYVGEDVESILSRL
                     LQVCNYDVSAAEKGIVYIDEIDKIARKGDNPSITRDVSGEGVQQALLKMLEGTEVMVP
                     PQGGRKHPEQKMVKINTQNILFICGGAFDGIEKIIARRTATQLIGFKRNEDETAVDRE
                     NLLQYIAPQDLRAFGLIPELIGRLPVLTHLDPLDATTLRAILITPKNAIIKQYEQLFL
                     LEGIELKVDDAVLDFIVDKAIEFKLGARGLRSICEALLTDAMFDLPSSPDAKVFHLTL
                     EVAQQKFNQSKLSLLKIAS"
     gene            complement(6628..>6826)
                     /locus_tag="PLL28_11920"
     CDS             complement(6628..>6826)
                     /locus_tag="PLL28_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNF70076.1"
                     /translation="ASDMEISLKEILKLKQELYNIISSHSGQPFDKVEKDSDRDYWMR
                     AEEARDYGMIDEVLVRDPKKS"
BASE COUNT         1941 a         1404 c         1446 g         2035 t
ORIGIN      
        1 atattacttc ccaaatcccg cttatggctt cccagcaacg tcacgtccag catttgtatt
       61 taagagccgg ctttggtgaa tcacccgcta ttgtcaagca gcgctcggct aaagaaatta
      121 agcaactggt tgatgattta tttactgaaa gtaagcaata tcgcaatctc acgtatctcg
      181 aaaatcctgc aaaagggaag gaagtttcaa acctgaaagt attgttgctc atcctgaaat
      241 cacaagagca gatgcgcgaa ttaaataatg tgtggatggg tatgcttgca tcgacgaaag
      301 ctgtgctgcg cgaaaaaatg accttattct ggcacgatca ttttgccacc tctacgcagt
      361 ttgcttattt gatgcagttg caaaataata cgctgcgaaa acatgcactc ggtagattcg
      421 ataagctgtt acacgccatt gctaaagatc ctgcaatgat tatttatctg aataatcagc
      481 agaacaaaaa agatgcaccc aacgaaaatt ttgcacgtga ggtaatggag ttgtttacgt
      541 tgggtgaagg aaacaaatac actgaggcag atatcaaaga agcggccaga gcatttacgg
      601 ggtggacggt gaataaaaag ggtgaatttg aatttgttgc aaaggatcac gacgatgaaa
      661 gcaaaacatt tcttggaagg gcaggaaatt tcaatggcga ggatataatt aatattttgc
      721 ttgaagaaaa gcaaacagca aaatatgtta ctaccaaaat ttataaggag tttgtaaatg
      781 ataatgtgaa tgatgcgcat gtagaggcat tgtcgacaat atttttcgat agtggttatg
      841 atatttcagc gttgatgcgt aatattttta cggcagattg gttttatgcc gatgaaaaca
      901 tggggtccct tatcgcatca cctgttgatt taattactcg ctttcgtagg ttgataaaga
      961 tggaatttaa agaagagaag ggtcagttta atacacaaaa aatactagga caggtattat
     1021 tttttcctcc gaatgtggca ggttggaagg gcggaaggaa ttgggtcgac agctctacgc
     1081 tcatgttgag aatgcaaatt cctacacgta ttatcagagg tggcggattt aatgttcgac
     1141 ccaaaccgga atttgaagat gcgcctgaag atgagatgac attgaaaacc tccaaaaaag
     1201 cagatgtgcg ctctgattgg tcgggagtag taaattattt taaggatgtc cccaccgatc
     1261 aactgactga gagcgttatc gaaacattta ttcaatcctc cagcgaccat atcgacagaa
     1321 atattattgc ccaatatgtc gataatagca gtgacgaaaa acgcatttta agcaccattg
     1381 gcgtggtgat gtctttacct gaatttcaac tcatctgata tggcaccaaa tagaagagat
     1441 tttctcaaac aaacaggatt ggcatcagca gggatattgg tgccttcatt cattaaggct
     1501 tccgtgccat ttctgccggg agatgtaaga ccaaaagtat tggtgatgat acagtttacc
     1561 ggagggaatg atggattgaa ttgcatcgtt cccatcggca acgacctgta tcataaggct
     1621 cgtccgaata taggttataa aaaagaagaa ctcatcaagc tgagtgatga tatcggcatg
     1681 aatgcaaatc ttcaaggctt agctgatctg ttctacgaag ggaatgtggt actgattaat
     1741 aatgttggat atcccgatcc taaccgctcg catttcagat cgatggatat ctggcaaact
     1801 gcaagtgatg aaaatgtgta tatgcaaaca gggtggatag gcaggatgtt agatcaatca
     1861 tgtaaaagtg gagactgcca aaagccgcat tttgctgttg agatcgacga cagcctgagc
     1921 cttgcattaa aaggtgaatc gatgagtggt tttgctgttc gtaatccgga ccaattgaag
     1981 aatgtgaatt taaatccgtt cattgatagt gttgcccgaa gcggcggcga tatacataca
     2041 catgataatg ttggttattt acataaattg ctgacagaca ctactgaaag cacggattat
     2101 atttatgatc agtcgaaaaa atatacaaca gcgtttactt atcctacatc ggaatttgca
     2161 gcacagttaa aaattattgc taacctgatt aatgcggaaa caaacacttc tgtgtattat
     2221 gtatcgttaa gtggattcga tacacatgcg ctgcaacgtg gacaacagga acgtcagtta
     2281 aggatttatt cggaggcctt gcaggcgttt acgaaagatt taaaatctac aaatagattt
     2341 aaggatacac tgatcatgac gttttctgaa tttggcagaa gggtggaaga aaatgccggt
     2401 aaaggaacgg atcatggtac tgcgaataat gtatatttaa ttggaggtgg aatgaagcag
     2461 ggcggcatga taaatgatat gcctgattta tcgaagttgg tagatgatga tttagtttat
     2521 caaacagatt tcaggaatat ttatgctacg attcttgata aatggcttga ggtgccgtcc
     2581 aaagaaattc tcggacgaga ctttgccagg atggactttt tgtgaccaat actgagtaaa
     2641 gcattcggtt tttattaaca tgagcctctt gatcggtttc gggatgaagg tttggaaatt
     2701 tttgcctttt aagtatttct aagcgaggtg tgctaagcgc ttgtattcga gtagaagcgg
     2761 ttgctcgcgt tagattcaat gtctgcctga cattccagat ttaagaccta acaggtttga
     2821 gacctgttag gtttaaaacc tgttaggttt aaaacgtgtt aggtttaaga cctgttaggt
     2881 ttaagacaat gggatgattc aattaccccc ccccattcaa cctccataaa ttatcaaaag
     2941 aaaatatttc gattcgggca actgcaccct cattctcacc atgcatcttc taaacacacc
     3001 actcgctact caagagtcac ggcattagta tgtagaggca agtatctaga atttgtatca
     3061 ggtatcaggt atatagtatc aagtatcaag tatcaagtat caggtatttg tatactcgca
     3121 tcacaaaccc aatacttaag cacgcaccac actcaccact caccactcac caccctcacc
     3181 actcaccact cacacttgct tcgctaccgt cttattcttc aggcgattga gcactttttc
     3241 ccataaaatc tcgtaaggaa ttatttccca ttcattcacg atattgtttc cactcggatc
     3301 gccggccatc attttacgaa gatatttctg agccatttgt ttcgtgcgat caaaaacaaa
     3361 atcttccttc accatagtaa gcagcatttt gagaaagcaa ctgcttcgat aggcgtcgtc
     3421 tttgcgcaag tagcgactgc aatatacatt gagtgcttca gagcggttga taatgccgga
     3481 gtattcccct ttttctaaca tgtataatat ttggatcacg agaatcgaca cattgtatcc
     3541 gcgtttatcc ttgctaaagt ttggtacttc attgaggaat ttcagcaaac ggaatttgcg
     3601 tttcgtagca tccgttgcca acaggtcgcg atcgagattt tctgtttcga agatgtagtt
     3661 gatataagct tcgaatattt tccatttctc cttgcgctca tctcctgtgt acatgaagcg
     3721 cgggtgattg attactttaa tgaaaatggc tgctgcgttg atatagttct cggtatgcat
     3781 ggccagcagg aaataatatt cgaggaaaac aaaccagtta ttacttcctt cctgcaccaa
     3841 ttcaagacac ttttctgcat tgcgttttcc attgtcgtaa tcacgcaatt gcagatatgc
     3901 acttattttg atgagtgcga gattggcgag ttttatcttt tgattgaagt tgggattact
     3961 ctccagataa ttttctgcct cttcacatac gcgaatagtt tccttaaact gccctttaat
     4021 ttgtgtacct atgatccaaa tctgatagcg catataatgc aattgatagc tatcagtttg
     4081 tttaatgagt ttgtttactt catcaagcga tttatcggca agcgctttta gttccggatc
     4141 actgtaaccg gcggcggcga aattgatact cagctgttga tataattcct ccgaacgtat
     4201 ctcaaagccc atgtcgcgca atgccttgtg ggtgaggtcg gcataataat cgtattcatt
     4261 atcatctcca ctaaggctgg cgttgctcat caggatgcgc gcacaattga ggatcatatc
     4321 tgtgatcctg taggtttgcg ctacactgag tgttttggtg gcaattttta ttgccgagct
     4381 gcgggcgcca ctcgaaagca gtatcctgat ctgtgcccag ttcttattac aggaatagtt
     4441 tgcacggtga taatccgaaa atgacggatc gttgctgtcg agaaaaaaca atgtattcaa
     4501 caaacgctta gagaagcgcg actttaactg acgatacttg tcatctaatg gcgaggtttc
     4561 atacagtatc gaagccgcct cttcatcggt ggtaaatctg tcgttgacca acccttcgta
     4621 aaattcattg aacttgctgt cgcgcttatt caacagactt ttatcgaaga tctcaatttt
     4681 agagatccgt ttctttgtca caatcttgga gatctcaatc aggtttttca tcgggttgtc
     4741 cgggttatct tgtgtgtgat aattggttaa atccttggtt ttgttgagtt tcgtgcgaat
     4801 ggctccaaac attcatgtca caaattaccc tacaatacca caaacaaata tgaatattta
     4861 accgcatatt cgcaacaagt tatccaccat ctatgccgat ttacgcggtt atgggacagt
     4921 attccgattg caaaaatgtc acaaaaaaat aataaattgt aattttaaaa ccgataaata
     4981 aggcatttag cgcgaataat cggttataac cgcttaatta atcatgtcac aaaatgtcat
     5041 ttgctgaaaa tgatttaccc attcatctgg ttacttttga tttgtttatt tgacagcctt
     5101 ttaaataata cagcgtatga caacttttac actaccgaat tttcccgttc aggccatcgt
     5161 gatcgtagac ctggacgagc tatagaaaga cagttggttt gtttcataaa ttaggttcac
     5221 gaccgaaagg aggcgcaagc ctccttttgt gttttatggc ctgtcacaaa tgatttacct
     5281 actccataaa acaacaatag cctcatcaag aggctatcgc gtatattttg ttgctcgtcg
     5341 tttatcagga ggcaattttc aataaactga gcttcgactg gttgaacttt tgctgcgcca
     5401 cttctaatgt cagatgaaat accttggcat cgggtgatga cggcaaatca aacatcgcgt
     5461 ctgtcagcaa ggcctcgcaa atactgcgca gtccgcgggc gccgagtttg aactcaatag
     5521 ccttatctac aataaaatct aaaacggcat cgtcaacctt caattcaatg ccttcgagca
     5581 ggaacaattg ttcatattgc ttgatgatgg catttttcgg ggtgatgaga atggcgcgca
     5641 gggtagtagc atccagtgga tcgaggtggg ttaaaaccgg cagtcggcct attaattccg
     5701 gaatcaatcc gaatgctctc aagtcctgag gagcaatata ttgtaacaga ttttcacggt
     5761 ctacagccgt ttcatcctca ttgcgcttga accctatcaa ttgggttgcc gtgcgacgtg
     5821 caatgatctt ttcaatacca tcaaaagcgc ctccgcaaat gaacaggatg ttctgggtat
     5881 tgatctttac catcttctgc tcagggtgct tacggccgcc ttgaggcgga accatcactt
     5941 cagtcccttc aagcatcttt aacaatgcct gctgaactcc ttcgcccgaa acatcgcgtg
     6001 taatacttgg attgtctcct ttgcgcgcta ttttatcgat ctcgtcgata tatacaatac
     6061 ctttttctgc cgccgaaacg tcgtagttgc aaacctgtaa taacctgctc aggatactct
     6121 ctacatcttc acccacatat ccggcttcgg taaacaccgt cgcatcaaca atggtaaaag
     6181 gcacattcag gaatttggca atagatcgcg ccagcaaagt ctttcctgta cctgtgcgac
     6241 caaccaccat aatgttcgat ttctcaatct ccacatcttc aagcggcttt tgattcagtc
     6301 gcttatagtg gttgtaaact gccaccgaga gtatcttttt cgcctcgtcc tggccaatta
     6361 cataggtatc cagaaactct ttaatagagc gaggtttcat cgaattggtc aaagcgaatt
     6421 tatagcgctt taccggttgc atcagctctt catctaccat ttgctgtgcc tgtgctacac
     6481 agttttcgca aatatgtccc tcttgtccgg cgatgagaat gcgcacatcc tccttcgggc
     6541 gaagacaaaa cgagcagtaa gcggagtctt tctttttcat tccgtaaggc gttatagatc
     6601 aaaagtaccc tgtttcaggg aattttatca gctttttttc gggtcgcgaa ccaaaacttc
     6661 gtcgatcata ccgtaatcac gagcctcttc ggcgcgcatc cagtagtcgc gatcggagtc
     6721 tttttccacc ttatcgaatg gttgtccgct gtgcgagctg atgatattat acagttcctg
     6781 cttgagtttc aggatttctt tcaatgaaat ctccatgtcg ctcgcc
//
LOCUS       DAOQHY010000166         6646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_6153_length_6646_cov_5.666212, whole genome shotgun sequence.
ACCESSION   DAOQHY010000166 DAOQHY010000000
VERSION     DAOQHY010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6646
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6153_length_6646_cov_5.666212"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..144
                     /locus_tag="PLL28_11925"
     tRNA            71..144
                     /locus_tag="PLL28_11925"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:107..109,aa:Arg,seq:acg)
     gene            746..1474
                     /locus_tag="PLL28_11930"
     CDS             746..1474
                     /locus_tag="PLL28_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70077.1"
                     /translation="MQKLIWISIFICTILSCKESNITAQMHQGEHLVIVDSVDAESRF
                     VDYDFYKRHGVYSIYYLGADTDTIQLGKHKINGFDDGYEDFQNRNDFTLPDPSKLSIY
                     VDTLIPLKQIIRVSGKGIRYYELKDTLVINAFPIIVRNHCDSLIRLGIFSEICYMVRQ
                     AQDETGDWVDIENTIRFGCSTGARDIVIKPDSLIIALLPRYEGDYKTACRLRFMHGKH
                     AVYSNVFYDMIDKRQFSEPLPDQY"
     gene            1479..2099
                     /locus_tag="PLL28_11935"
     CDS             1479..2099
                     /locus_tag="PLL28_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70078.1"
                     /translation="MHDYKMKMNSSILMLCVILLSACTSNHVSEPRFQFSQIDIVSYK
                     WTSDTIQHDWQLYIASIGSIDSLGQCAIARRDTFKGTERYFKQVLPMAFDSLIAKINY
                     KELPTSFFIKDDVVRIYDGFTYCLLWHQQDTIVSTVFEPDKIPHGALRDFIMQLNDFI
                     QQADTESSTSFDYSEINRYILEHVVDSSRLPPYRYVIEFKRTDSPK"
     gene            complement(2125..2295)
                     /locus_tag="PLL28_11940"
     CDS             complement(2125..2295)
                     /locus_tag="PLL28_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70079.1"
                     /translation="MGKDLVKERREERGERREEKGERRKEKGERRKERGDKCKEMEEI
                     LVDTVVCKNCDI"
     gene            complement(2277..3383)
                     /locus_tag="PLL28_11945"
     CDS             complement(2277..3383)
                     /locus_tag="PLL28_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70080.1"
                     /translation="MKKVNLGIAQNKFDQIQQRSENVLHLSIAKLVGHIENPTTYPMT
                     GDNKSIERALFNHYNILAKSEQKDFINRIKKFSNIPSNTTQFQSRFGDLAKVDLKSHT
                     SVAEQIKSIAVPDSLKFTASEIDELEKKLLKKRVGAKSTPVTKQPAVVAPRLIRLTVE
                     SIHCTNTVELKKDEILMNALFTDALGVSTTIDLVDVGKMKDGETINLGAKGIIAEVNL
                     NNAGFFPQTLIGTFTLIDKDVFADQEKIVSAFRTCAIIATTLTSIALISMGVAIAVAA
                     TPGGMAAAAGIMTGALIAMVGSLVILVVGGGITLALQGETSDLIKDEFVFDLNPVTLQ
                     PGEFVTRVLNVEIPRSIGKKSGNYTMTIKWERIS"
     gene            3582..4979
                     /locus_tag="PLL28_11950"
     CDS             3582..4979
                     /locus_tag="PLL28_11950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNF70081.1"
                     /translation="MTCAIVGGLTLPLLIQDGMFMDALLYTSVANNLSTGYGTFWFPQ
                     FSLHNVSDFNAFHEQPPLGIFIQSLFFRAFGHSMYVERFYTFLMLIITARLMVSLWKR
                     VYLSESILHQYSWLPVLLWMLSPICFWSYSNNMLENTMGVFTLSAVILLFDAMRSQKT
                     SKFILGGLFIFLATMIKGVPGLFPLATPCIIWIMERNSGFVKMIWQSFLSFITVALLY
                     CCMLLIPDARESYDVYFLQRTLFRIDSLPTVNSRFYILGRLFLELLPQFILLIIVLLI
                     ARNKKRIPGAQYFKQGLIFILIGISASLPLMLTMVQKAFYFVPALPLFAIGLASMMAP
                     VIAIENRKENLRPSTLKVWRGVGVLLFIGMLGYTGAMFGHVKRDKDLIHDVRLIGDAI
                     PNEHVIGTPNAMWNNWSMQTYMMRYNNMSMDEGYKQTYVVVDQKYQTQIPEFYTKIDI
                     PTKRYHLFKRKESGI"
     gene            4985..6610
                     /locus_tag="PLL28_11955"
     CDS             4985..6610
                     /locus_tag="PLL28_11955"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNF70082.1"
                     /translation="MQTRNKIFYFLAVCSLFVVACTHHDTEDKKIFHLNLASPVTSLD
                     PAFSSDQPNTWSVNLLFNGLVQLDSDLQVQPCIASHWELSPDRLRYTFHLRNDVYFHN
                     DACFPGGKGRRVVAEDVVYSFHRLIDPEVAARGNWVFNNIVDTLLPFTAINDSTIEIR
                     LLKPFAPFIQRLSIPYCSVVPHEAIEKYGLDFRSHPVGTGPFVFKQWDEGELIILHRF
                     DNYFERDNAGNRLPYLDAVNIHFIANKSTEFLKFLSGDLDFVSDIDAAMKEDILTKEG
                     NLQDKYAEKINLLKGPYLNVEYFSILMDTSNVLVKSSPLSMEEVRKAINYGFDRKEML
                     LFLKNNRGVAATRGIVPPELLPAGPVGYGYDYHPDSALVLLKRAGFENGVGLPEIVIQ
                     TIEQYQDIAVYLKDKLEDIGIAIRIETIDPRLMREMRLNGTTVFFRSSWIADYADAEN
                     YFTLFYGLSDAPPNYTRYKNATFDSLYTIAVAEPDAVVRGKLYRSMDSLIMEDAPVVP
                     LFYDEIYRFTQKNITGLEPNGLNMLDLRRVKKD"
BASE COUNT         1862 a         1330 c         1350 g         2104 t
ORIGIN      
        1 aagcaggcga ggcaggcccg ccttgccaag ccgtatgcta agcaggcgag gcaggcccgc
       61 cttgccaagc cgtatgctaa gcaggcgagg cagggagcat ctgactacgg atcagaaggt
      121 tacaggtttg aatcctgtcg aggtcaatta atccatctat aagcgggatg tttttttgga
      181 ggaaggaatg attatgttgg gagagtaact tgtatgcaag tatatgtatt aaaaagtctt
      241 atagccaaac ggttttacgt tggcatgacc atggatgctt ataaccgatt agcccaacat
      301 aatgcgggca tgactagatc cacgaaatat cttaagccat ggataattgt ccatgttgaa
      361 gaatttccgg atgctgcttc agcaagaagg cgagagaaat atttaaaatc aggagctgga
      421 agacgatacc tgaagggatt aaacttgtaa caaatgccaa caacggcccg ataaacccaa
      481 tgcaaagctg gtgtggcgat ctgacaatgc tttaataaga aggatagtgt ggttacaata
      541 gatttgaatt cagaagcttg cctcgccaag ccaaatgcaa agcaggtgag gcggggttac
      601 aggtttgaat cctgtcgagg tcacttttct tccttcgttt tcttggagta tatttggtag
      661 ggtagcgctg tctagtatct agtctggtaa cagcttggcg cctttgccac tttgcgtctt
      721 agcgcgagca aaacacatcc atcgcatgca aaaacttatc tggataagta ttttcatctg
      781 caccattctt agttgtaagg agagcaatat tactgcgcaa atgcaccaag gcgagcacct
      841 tgtaattgtg gattctgttg atgcagaaag caggtttgta gattatgatt tttataaacg
      901 acatggcgta tattcaattt actacctggg tgcagatact gatactattc aactcggcaa
      961 acacaaaata aacggctttg acgacggcta tgaagatttt caaaaccgca atgattttac
     1021 cttgcccgat ccctcgaaat tgagtattta tgtcgacacc cttattcctt tgaaacaaat
     1081 catcagggtt tcaggaaaag gtattcgtta ctatgaatta aaagataccc ttgttattaa
     1141 tgcctttcct atcatcgtca gaaatcattg cgattcattg attagactag ggatattcag
     1201 cgagatctgc tacatggtcc gtcaggcaca agatgaaacc ggggattggg tggatatcga
     1261 aaatacaatt cgcttcggat gcagcaccgg agcgcgcgac atagtcatta aacctgatag
     1321 cctgattatc gcactgctgc caagatacga aggcgattat aaaactgctt gccgacttcg
     1381 atttatgcac ggcaaacatg ctgtgtattc caatgtgttt tacgatatga ttgacaaacg
     1441 acaatttagc gaaccactgc cggatcaata ttgatattat gcatgactat aaaatgaaaa
     1501 tgaactcgtc aatcttaatg ctatgtgtca ttttattaag tgcatgcaca tcaaaccatg
     1561 tttccgagcc caggtttcaa ttttcccaaa tagatattgt atcatacaaa tggacctcag
     1621 ataccattca acatgattgg caactctata tagcatcaat cggcagtatc gacagcttgg
     1681 gtcaatgtgc catcgcgcgt agagatacat ttaaaggtac agaacgatat ttcaaacagg
     1741 tacttcccat ggcatttgat tcactgatcg ctaaaataaa ttataaagaa ttacctacat
     1801 cattttttat aaaagacgat gttgtgcgca tctatgatgg atttacttac tgcctgctat
     1861 ggcatcagca agataccatt gtatctactg ttttcgaacc ggataaaatt ccacatggcg
     1921 ccttacgcga ttttatcatg caattgaatg attttattca acaggctgat actgaaagtt
     1981 caacgtcatt cgactattct gaaatcaatc ggtatatact agagcatgtt gtggattcat
     2041 caagattgcc accgtatcgg tacgtgattg aatttaagcg cacagactcg ccgaaataag
     2101 gtagcaaaac ggttgccaac cgccctaaat atcgcaattc ttgcagacca ctgtatctac
     2161 taatatttct tccatctcct tacacttatc tcctctttcc tttctccttt ctcctttttc
     2221 ctttctcctt tctcctttct cctctctcct ttctcctctc tcctctctcc tctctttcac
     2281 gagatccttt cccacttgat tgtcattgta taattccctg attttttacc gattgaccgt
     2341 ggaatctcca cattaaggac tctggtaaca aattcacccg gctgtaatgt tacagggttt
     2401 aagtcaaata caaattcatc tttaatcaaa tccgaagttt cgccctgaag tgccagggtt
     2461 attccaccgc ctacaactaa tattaccaaa gagccaacca tggcaattaa tgcacctgtc
     2521 ataatgcctg ctgccgcagc catacctccc ggagttgctg ctacagctat tgcaacaccc
     2581 attgatatta atgcaatgga tgtcagtgta gtggcaataa tggcacatgt cctgaatgca
     2641 ctgacaattt tttcctgatc agcaaaaaca tctttatcaa tcaacgtaaa tgttccgatt
     2701 aaggtctgag ggaaaaatcc tgcattattt aggttaactt cagcaataat acctttagcc
     2761 cctaaattta ttgtttcgcc atccttcatt ttgcctacat caacaaggtc gatcgtggtt
     2821 gatacaccta atgcgtcggt aaacagggca ttcatgagta tttcgtcttt ctttaattca
     2881 acagtatttg tgcaatgtat gctttcaact gtcagtctaa ttaatcgtgg cgcaacaact
     2941 gcaggttgtt ttgtgacggg tgtggatttt gctccaacgc gcttttttaa taacttcttt
     3001 tcgagctcat cgatttctga agcggtaaac tttaaactgt cgggaactgc aatacttttt
     3061 atttgttctg ctactgaagt atggcttttg agatccacct tcgccaaatc accaaagcgc
     3121 gattgaaact gagtagtatt tgatggaata ttgctaaatt tttttatgcg attgatgaaa
     3181 tccttttgtt cggattttgc taatatgtta tagtgattga ataatgcacg ctctatcgac
     3241 ttgttatcgc cggtcattgg atatgttgtc ggattttcaa tgtgccctac caatttggca
     3301 atcgataaat gtaagacgtt ttcactacgc tgttggattt gatcaaattt gttttgagca
     3361 atgcctaaat tgactttttt catgttaatg gttttgtgtt ttgagattgt tattagttct
     3421 ttgagtctgt attaaaggta ataatttgta taacttaaat ccatatttat ttcccgtttt
     3481 cttattcatt tcagccttaa tagtggacta aacctcgtat tttagcactt gcgatgccat
     3541 tatcggagaa ttcctcatcc ttccacagat ccatgtggct tgtgacctgt gccattgtgg
     3601 gcggacttac actgccactg cttattcagg atgggatgtt tatggatgca ctgctataca
     3661 cctccgtagc taataacctt agcaccggtt acggaacatt ttggttcccg cagttcagcc
     3721 tgcataatgt atctgatttc aatgcatttc atgagcaacc tcctctgggg attttcattc
     3781 aatcattatt cttcagagca tttggacata gcatgtatgt agaacggttt tatacttttc
     3841 tcatgctgat tattaccgca cgattaatgg tatccttatg gaagcgcgtt tatctttctg
     3901 aaagcatatt acaccaatat tcctggctgc ctgttttatt atggatgctg tcgccaattt
     3961 gcttttggtc ttacagcaat aatatgctcg agaataccat gggtgttttt acgctgtcgg
     4021 cagtgatttt gttgtttgat gccatgcgca gtcagaaaac atcaaaattt attttgggtg
     4081 gattgtttat ttttcttgcc actatgatca aaggagtgcc cggtttattt ccgctggcaa
     4141 caccatgcat aatctggata atggaaagaa attccggctt tgttaagatg atttggcaat
     4201 catttttatc attcataaca gtggctttgc tttactgctg tatgctcctt attccggatg
     4261 cacgtgaaag ctatgatgtg tattttttac agcgtacttt attccgcata gattcacttc
     4321 caaccgtaaa tagcaggttt tatatattgg gcagattatt tcttgaacta ttgcctcagt
     4381 ttattttgct tatcattgtg ttgctgatcg cacgcaataa aaaacgcatt cctggtgcgc
     4441 agtattttaa acaaggattg atatttattc taatcggtat ttcagcatct cttcccctca
     4501 tgctcaccat ggtgcaaaaa gcattttatt ttgttccggc acttcccttg tttgctatag
     4561 gattggcttc aatgatggca ccggttatcg ctattgaaaa tcgcaaggaa aatttacgtc
     4621 cctcaacatt gaaagtatgg cgtggtgttg gtgtcctttt gtttatcggc atgctcggct
     4681 ataccggtgc tatgttcggt catgttaagc gcgataaaga tcttatacat gatgttagat
     4741 taattggaga tgcaattccg aatgagcatg ttattggcac accgaatgcc atgtggaata
     4801 actggagcat gcaaacttat atgatgcgtt ataacaacat gagtatggat gagggttata
     4861 aacaaactta tgtggttgta gatcagaaat atcaaacgca aattccggaa ttttatacca
     4921 aaatagatat cccaactaag cgatatcatc tgttcaaaag aaaggaatca ggcatctagt
     4981 gatcatgcaa actcgtaata agatatttta cttccttgct gtatgcagct tatttgttgt
     5041 ggcatgtaca catcacgata cggaggataa aaaaatattc cacctcaatc ttgccagtcc
     5101 tgttacatcg cttgaccctg cgttttccag cgatcaaccg aatacctgga gcgtaaattt
     5161 attgttcaac ggacttgtgc aactggatag tgatcttcaa gtgcaaccat gtattgcatc
     5221 tcattgggaa ttatcgcctg accgcttgcg atataccttt cacttgcgca acgatgttta
     5281 ttttcataat gatgcatgtt tccccggtgg aaagggtagg cgagtggttg cagaagatgt
     5341 ggtgtattca tttcaccgac ttatagatcc tgaagtggca gcacgtggaa attgggtatt
     5401 taataatatt gtcgacacgc tattgccatt tactgcaata aatgattcaa caatagaaat
     5461 taggctgctt aaaccttttg cgccatttat tcagcgattg agtattccgt attgttctgt
     5521 cgttccacat gaagccattg aaaaatatgg acttgatttc agaagtcacc ctgtcggaac
     5581 ggggcctttt gtatttaaac aatgggatga aggtgaattg atcatcttac atcgattcga
     5641 taactatttt gaacgcgata atgccggaaa ccgtcttcca tacctcgatg ccgtaaatat
     5701 tcatttcatc gcaaataagt cgaccgaatt tttgaaattc ctcagtggcg atctggattt
     5761 tgtgagtgat attgatgcgg ctatgaaaga agatatcctc acgaaggaag gcaatttgca
     5821 ggataaatat gccgagaaaa taaatttatt gaaaggtcct tatctcaatg ttgaatattt
     5881 ctccatttta atggatacct ctaatgtatt ggtgaaatca agtccgcttt ccatggagga
     5941 agtgcgcaaa gctattaatt atggtttcga tagaaaggaa atgttattgt tcctcaaaaa
     6001 caacagaggt gttgccgcaa ctcgtggcat agtgcctcca gaattgttgc ctgccggacc
     6061 ggtaggatat ggatacgact atcatccgga tagtgcacta gtgctgctta aaagagcggg
     6121 gtttgaaaat ggcgtaggcc taccggaaat tgtaatacag actatagagc agtatcagga
     6181 tatcgcagtt tacttaaaag ataaacttga agatatcggc atcgccattc gcattgaaac
     6241 catcgatccg agattgatgc gtgaaatgcg attaaatggc acaacagtat ttttcagatc
     6301 aagctggatt gcagattatg cagatgctga aaattatttc actttgtttt atgggttaag
     6361 cgatgcacct cctaattata caaggtataa aaatgccact ttcgatagct tgtatactat
     6421 tgcggttgca gagcccgatg cggtggtgcg cggaaaatta taccgcagca tggacagcct
     6481 cataatggag gatgcgccgg tggtgccatt gttttatgat gaaatttatc gcttcacgca
     6541 aaaaaatatc acaggtctcg aacccaacgg attaaatatg cttgacttgc gaagagtgaa
     6601 aaaagattga gcaattattg atgttcacac aaaagtgaca ctacct
//
LOCUS       DAOQHY010000167         6613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_6216_length_6613_cov_4.767307, whole genome shotgun sequence.
ACCESSION   DAOQHY010000167 DAOQHY010000000
VERSION     DAOQHY010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6613
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6216_length_6613_cov_4.767307"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PLL28_11960"
     CDS             <1..333
                     /locus_tag="PLL28_11960"
                     /inference="COORDINATES: protein motif:HMM:NF019159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HNF70083.1"
                     /translation="AIEHPENTILIVGYCADNTPGGQLRNGAKQIRMFGEYKSVNAHV
                     EIMRSFSAHGDYREMIDFLRSQEKDKLRNIMLVHGDKNALTGFRDHLHEAGFGQVDIP
                     KLGDKVDL"
     gene            complement(353..853)
                     /locus_tag="PLL28_11965"
     CDS             complement(353..853)
                     /locus_tag="PLL28_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70084.1"
                     /translation="MKHLLSFFAISLFVISTHAQDWEVPRHYMLEGAEDYVPYEADII
                     QAANWLRDVSPTTDVEKFKEASQFVIKWVNGSPTVTVEINKTIMEFNEKNHNMLVLYM
                     ASCAKYVLENNYSKDVQAKHISALKDMAIAYQKQPQMQKDKKMEKLVKAIEEGTADDW
                     YNKNFK"
     gene            complement(928..1470)
                     /locus_tag="PLL28_11970"
     CDS             complement(928..1470)
                     /locus_tag="PLL28_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70085.1"
                     /translation="MKKITYLFILLPFLISINSCKNEETPDPVNEEELITSVVLSFEN
                     NADLSTSTFAFRDIDGPGGDAPSEFDTIRLNNGYYHLTVSILNESVSSALDLTAEIDE
                     ESTDHQLFYTVDGADITFDYNDTDANGLPIGLDMNVAVGPTSSTGTVTVVLKHQPGIK
                     DGSISTGESDVEVTFVTETN"
     gene            complement(1497..3824)
                     /locus_tag="PLL28_11975"
     CDS             complement(1497..3824)
                     /locus_tag="PLL28_11975"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF70086.1"
                     /translation="MKHVLFVCVMMSALTQLKAQSDNCNLRFSGRAVDMDHKEPLSYA
                     TLYINETQTAVVADENGKFMFEGLCPDTFTLVISHIGCTTSTFTIALTEDTFKEFRLD
                     HTSENLESIVVHGEQDKNTGTLTETTLSGKKLDRLSGKSLGEALKDVNGVSSLQTGNG
                     ISKPVIHGLHSNRILIMNNGVRQEGQQWGNEHGPEIDPFIANSLTVIKGAASVQYGSD
                     ALGGVILVQPAPMPTQKGVSGDLHAVGFSNTRGGAASLMLQYKASDSAALSYRLQGSI
                     KREGNVKTPNYFLANSALAEQSGAATIDYHRNNLHSQVYYSYFHNKVGIFSGSHIGNL
                     TDLQTAFASDTPLVHQDFTYIIGRPYQDIVHHLIKNETNFHHERLGTFSLITAWQNDL
                     RSEYDAHTSLIDSIAALGLPSLYFEIGTITADLKWEQHPGKRHNAVIGISGMNQQNIT
                     RYSIFIPNFKNYSGGIYAIENYKFGRVLLEGGIRYDYKWMQIFRYEGNTLETPTHQYG
                     NLSGIAGMQFFDGRHTQYNLNFSSAWRSPSVSELYSDGLHHGSASLEYGNDSLVSERA
                     YQIIAGIEHQSDKWYFDVGVYNNFIYNFIYLEPTLPAELTIRGAFPVFHYMQTNANLS
                     GADVQTSYMIIPQLKAEIKASTLRARDVANNKWLVMMPADKISFDLEYQPADFKWMHD
                     NAIHAGVEYVFEQKRAPLDEDYVPPPSAYTLFSAGIEGVIALHANEISWSLSADNLLN
                     TAYRNYMNRFRYFADDMGRNITLRIKIPITGKSNL"
     gene            complement(3872..4228)
                     /locus_tag="PLL28_11980"
     CDS             complement(3872..4228)
                     /locus_tag="PLL28_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70087.1"
                     /translation="MMQFLRRSSALSLIATILLLGALSGKDLVHFTFAHDVSHQYGSD
                     SRECCKQDHRQPHIHELNYCFLCHLEVSVTLHEERVVLIPGSFSYDLIPVAHLNSVHQ
                     PGFQGYSDLRGPPFTV"
     gene            4395..6437
                     /locus_tag="PLL28_11985"
     CDS             4395..6437
                     /locus_tag="PLL28_11985"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HNF70088.1"
                     /translation="MRKYPLWLLAALMVSVSACKSTKAPEEDAYQAFDPASLDSTVRP
                     GDDFYAFVNNKWIAAHPIPADKAKYGAFYMLDDNSLTTLRSAMETAAKANAAKGTNTQ
                     KVGDFWTSGMDTNAIENAGYTPIKPWLDKINAIGNADELMRTVAQMHRTYTFAMFTNW
                     VGQDDKNSADVIMNMWQGGLGLPDRDYYTRTDAKSQQIRADYLVHLKNVFMLIGADDA
                     TASKNANTVMSIETALAKASMTNVEMRDPNKIYHKMPLADFQKLTPNMNWNVYYTELK
                     TPEFKTGLNVAQPDFYKALNGMMKSVSLNDWKTYLTYHFVDGASDYLSAPFVNEHFDF
                     HGKKLNGIEELKPRWKRVVETADFCLGEALGEEYVKVAFSPESKERMLEMVGNIKTAL
                     GQRIQNLEWMTDSTKQQALHKLSTFTVKIGYPDKWRDYSNLTITRDAYVLNVMAAAEF
                     EAQRNYNKIGQPVDKSEWGMTPQTVNAYYNPSNNEIVFPAAILQPPFFDPKADDALNY
                     GGIGAVIGHEITHGFDDQGRMYDANGNLNIWWTDKDNSQFVERANVVVTQFNGYCPLD
                     SLCINGELTLGENIADFGGITTAYQAYTMTEEFKAGKSIDGFTPQQRFFLGFGRIWAG
                     DYREDAMRTQLLTNVHSPGEWRVKGTLVNIPEWYEAFGVKEGDKMYLAPDQRAKIW"
BASE COUNT         1936 a         1278 c         1446 g         1953 t
ORIGIN      
        1 gccattgaac atccggagaa tacaatattg attgtcggat attgtgcaga taacactccc
       61 ggcgggcaat tgcgcaatgg ggcgaagcaa attcgcatgt ttggcgaata taaatcggtg
      121 aatgcgcacg tagaaattat gaggagcttt tctgcacatg gcgactaccg cgagatgata
      181 gattttttac gcagtcagga aaaagataag ctacgcaata tcatgttggt gcatggcgac
      241 aaaaatgcac taaccggctt tcgcgatcat ttacatgagg cgggtttcgg gcaggtggat
      301 attccgaagc ttggagataa ggttgatttg tagttgacaa atcaactgcg attcatttaa
      361 aatttttatt gtaccaatcg tcagcagttc cttcctcaat agctttcacc agcttttcca
      421 tctttttatc cttttgcatt tgaggttgtt tttgataggc tatggccatg tccttcagag
      481 ctgaaatatg tttggcctga acatccttac tgtaattatt ttcgagtacg tattttgcgc
      541 atgaagccat atacaatacc agcatattgt gattcttttc attaaattcc atgatggttt
      601 tgtttatttc aacagttacg gtgggacttc cattaaccca tttgataacg aattgcgaag
      661 cttctttgaa tttttctaca tcggtagtcg gacttacatc gcgcagccaa ttagcggctt
      721 gtatgatatc ggcttcgtaa ggtacataat cttcagcccc ttcaagcatg taatgacgtg
      781 gaacttccca atcctgggca tgcgttgaaa ttacaaaaag gctgatcgca aaaaaggaaa
      841 gtaggtgttt catgttataa gttttcgcta atgtacataa aatagcccat aagtgaatac
      901 gggctttttt tagaacgtat aaaatgatta attagtctca gttacaaaag taacttcaac
      961 atcagattcg ccggttgaaa tgcttccatc tttgatgccg ggttggtgtt ttaatactac
     1021 ggttacagtc cccgttgaag acgtaggtcc aaccgcaaca ttcatgtcaa gaccaatagg
     1081 taagccgttg gcatcagtat cattatagtc aaatgtaata tctgctccat caacggtgta
     1141 aaataattga tggtctgtag actcttcgtc aatctctgca gttaaatcaa gagctgaaga
     1201 tacactttca tttaatattg atacggtcaa atgataatag ccgttattca gtcgaatggt
     1261 atcaaattca gaaggggcat caccacccgg accatcaata tcccgaaatg caaaggtgga
     1321 tgttgacagg tcggcattgt tttcaaaaga cagcaccacg cttgtgatta attcctcttc
     1381 atttaccggg tcaggagttt cctcattctt acaagagttt attgaaatca gaaagggcaa
     1441 taatatgaac aaatatgtta tctttttcat aataatatta ttataggttg attgaattat
     1501 aaatttgatt ttcctgtgat tgggattttg attcttaatg tgatatttcg acccatgtca
     1561 tcggcaaaat atcggaatct gttcatgtaa tttcgatatg cggtattcaa tagattgtcg
     1621 gcactcaacg accagctaat ttcattagca tgtaaggcaa taacaccttc gattccggca
     1681 gaaaagaggg tataagcaga tggaggaggg acatagtctt catccagagg agctcttttt
     1741 tgttcgaaga catattcaac accggcatga atcgcgttat catgcatcca tttaaaatca
     1801 gcaggttgat attcgaggtc gaatgaaatt ttatctgccg gcatcatcac caaccattta
     1861 ttatttgcca catctcgtgc gcgcaatgtt gatgctttta tttcagcttt taattgtgga
     1921 atgatcatgt agctggtttg tacatcggca cctgataaat tggcatttgt ttgcatgtaa
     1981 tgaaaaaccg gaaatgcacc acgaattgtc aattcagcgg gtagggtagg ttcgaggtaa
     2041 ataaaattat aaatgaaatt attgtacaca ccgacatcaa aataccattt atcgctttga
     2101 tgttcaattc cagcgataat ctgatatgcc cgttcgctca caagactatc attcccatat
     2161 tccaacgaag cagaaccatg atgtaggcca tcgctatata attcactaac actcggcgaa
     2221 cgccatgcgg agctgaaatt taggttgtat tgtgtatgtc ggccatcgaa aaattgcatt
     2281 cctgcaattc cacttaaatt accatattgg tgtgttggtg tttcaagtgt attgccttcg
     2341 tatctgaaaa tctgcatcca tttgtaatca taacggatgc cgccttccaa taacactctg
     2401 ccgaatttat aattttcaat ggcgtaaatt cccccactat agtttttgaa atttggaatg
     2461 aaaatgctgt atcgtgtgat attttgttga ttcattccgc ttatcccaat aactgcatta
     2521 tggcgctttc cgggatgttg ttcccatttt aaatcagcgg taatagtgcc gatctcaaag
     2581 tacaatgaag gcaatccaag tgccgcaata ctgtcgatca gtgacgtatg tgcatcatat
     2641 tcacttcgca aatcattttg ccatgcggta attaatgaga atgtgcccaa gcgctcatgg
     2701 tgaaaattgg tttcattttt aatgagatga tgtacaatgt cctgatatgg tctgccaatt
     2761 atgtatgtaa aatcctggtg tactagcggc gtgtcagatg caaaagcagt ttgcagatcg
     2821 gtgaggttgc caatatgtga accggagaaa atacctacct tgttatggaa gtaactataa
     2881 taaacctgac tgtgcaaatt atttctgtgg tagtcaatag ttgctgcacc ggattgttcc
     2941 gctaaagcac tatttgcgag gaaataattt ggagttttta cattgccctc acgctttatt
     3001 gatccttgca gacgataaga aagggcggct gaatccgatg ctttgtattg caacatcagc
     3061 gaagcggcac caccacgtgt atttgaaaaa cctacagcat gcaaatcgcc tgaaacgcct
     3121 ttttgagttg gcattggcgc aggttgaaca agaataacac caccgagggc atcacttcca
     3181 tattgcacag atgcagcacc tttaataacg gtgagactgt tggcaataaa tgggtcgatc
     3241 tccgggccat gttcatttcc ccattgttgt ccctcctgac gcacgccatt attcataatt
     3301 agtatccgat tgctatgcaa gccatgaatt accggtttgg aaatgccatt tcctgtttga
     3361 aggctactca caccatttac atcctttaat gcttcgccta atgatttgcc ggacaatcgg
     3421 tctagttttt ttccgctaag ggttgtttca gttaacgtac ctgtattttt atcttgttca
     3481 ccatgcacaa caatactttc aagattttca cttgtgtgat cgagtcggaa ttctttaaaa
     3541 gtatcttcag ttagtgctat agtaaatgta gaagtggtac atccgatatg agaaattacc
     3601 aatgtaaaag tatcgggaca taaaccttcg aacatgaatt tgccattttc atcagccaca
     3661 acggctgttt gggtttcatt gatgtaaagc gttgcatagg atagaggttc tttatgatcc
     3721 atatccacag cgcgaccgct aaaccgaaga ttacaattgt cggattgagc tttgagttga
     3781 gtgagtgccg acatcatgac acacacgaat aaaacatgtt tcatacacga aataaaaagt
     3841 gatcggccaa agccaaataa attacagtag attaaactgt aaatggcggc ccgcgaagat
     3901 cactgtagcc ctgaaaaccg ggttggtgta cactattgag atgagcaacg ggaatgagat
     3961 cataggagaa ggatcccggt atcagtacaa cacgttcttc atgtaaagta acagatactt
     4021 ccagatgaca caagaagcag tagttcagct catggatatg cggttgtcta tgatcttgtt
     4081 tgcagcattc gcgactgtca gaaccgtatt gatgcgaaac atcatgtgcg aaggtgaagt
     4141 gcacgaggtc tttgccactt agtgctccca acaggagtat agttgcgatt agagatagag
     4201 ccgatgatcg tcgcaaaaac tgcatcatgg tgcaaaagta ggctattgtt acattttatg
     4261 acagaatttt gaggcttgcc tcgcagttgt gagcttaaac cttacctgtt ttaacaagcc
     4321 ttaacctgat ttaaccggcc gaccctgcat ttaggcgcag ggaattttat ctttgcgcca
     4381 ccaaacaata aaacatgaga aaataccctt tatggcttct cgccgccctt atggtgtcag
     4441 taagtgcctg caaaagcaca aaagcacctg aggaagatgc ttatcaagca tttgacccgg
     4501 cgtctcttga ttcaactgtg agacccggag atgattttta tgcatttgtc aataacaagt
     4561 ggatcgcggc gcatcctatt cctgccgaca aggctaaata tggtgcattt tatatgctcg
     4621 acgataatag cttgaccaca ttgaggtcgg caatggaaac tgctgcaaag gcaaatgctg
     4681 ctaaaggcac aaatacgcaa aaagtaggtg atttctggac cagtggaatg gatactaatg
     4741 cgattgaaaa tgccggttat acgccaataa agccttggct tgacaaaatt aatgccattg
     4801 gtaacgcgga tgagttgatg cgcacagttg cacaaatgca tcgcacgtat acattcgcaa
     4861 tgttcaccaa ctgggttgga caggatgata aaaatagtgc tgatgtgatc atgaatatgt
     4921 ggcagggcgg acttggtttg cctgatcgcg attattatac acgcacagat gctaagagtc
     4981 agcaaattcg tgcagattat ctggtgcatt tgaaaaatgt atttatgttg ataggcgctg
     5041 atgatgctac tgcttctaaa aatgccaata ccgtaatgag tattgaaact gccttggcaa
     5101 aggcaagtat gacaaatgtg gagatgcgcg atccgaataa aatttaccac aagatgccat
     5161 tggctgattt tcagaaactc acaccaaaca tgaattggaa tgtgtattac actgaattga
     5221 aaacgcctga atttaaaacc ggattgaacg tagcccaacc tgatttttat aaggcattga
     5281 atggtatgat gaaatctgtt tctttgaatg attggaaaac atatctcacg tatcattttg
     5341 tggatggagc atcagattat ttgagtgcgc catttgtgaa tgaacatttc gatttccatg
     5401 gtaaaaaact caatggcatt gaagaattga aaccacgttg gaagcgcgta gttgagactg
     5461 ccgatttttg tttaggcgaa gcactgggag aagaatatgt gaaagttgcc ttcagtcctg
     5521 aaagcaaaga gcgcatgttg gaaatggtag gaaatatcaa gacagcattg ggacagcgca
     5581 ttcaaaatct tgagtggatg accgacagca ctaagcaaca agctttgcat aagttaagca
     5641 catttactgt aaagattgga tatcctgaca agtggagaga ttattctaat cttacaatta
     5701 cacgcgatgc atatgtattg aatgtgatgg cagctgctga atttgaagcg caacgcaatt
     5761 acaataaaat cggtcagccg gttgataagt cggagtgggg gatgacgcca caaacagtga
     5821 atgcgtatta caatccatct aataatgaga tcgtttttcc tgcagcgatt ctgcaaccac
     5881 cattcttcga tcctaaagct gatgacgccc ttaattatgg tggaattggt gctgtaatag
     5941 gacatgagat tacccacgga tttgatgatc agggtcgtat gtatgatgca aatggtaacc
     6001 tgaatatctg gtggactgat aaagacaatt cacaatttgt tgagcgtgct aatgttgtgg
     6061 ttacacaatt caatggttat tgtccgcttg acagtctttg tatcaatggt gaacttaccc
     6121 ttggcgaaaa cattgctgat ttcggtggta ttactactgc ttatcaggct tatacgatga
     6181 cagaagaatt caaggcaggt aagtcgatcg atggatttac gccacagcaa cgtttcttcc
     6241 ttggattcgg tcgcatttgg gccggagatt accgtgagga tgccatgcgt acacaattgc
     6301 tcactaacgt gcacagtccg ggagaatggc gtgtaaaagg aaccttagtg aacatacctg
     6361 agtggtatga agcgtttgga gtgaaggaag gggataagat gtatttggct cctgatcaga
     6421 gggctaagat ttggtaattt gaaagggaag atgatattga gacggcctcc gggatggggg
     6481 ccgttttttt tgtctaaacc tttcccgtct taaacctaac acgtctcaaa cctgccaggt
     6541 tttaaaccta acaggtttga aacctaacag gtctaagacc tgttgatgct ctttttgaaa
     6601 gactcactcc caa
//
LOCUS       DAOQHY010000168         6596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_6250_length_6596_cov_4.675891, whole genome shotgun sequence.
ACCESSION   DAOQHY010000168 DAOQHY010000000
VERSION     DAOQHY010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6596
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6250_length_6596_cov_4.675891"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PLL28_11990"
     CDS             complement(<1..489)
                     /locus_tag="PLL28_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70089.1"
                     /translation="MKSQLLFLRSILLLGIVFFSTNVKSQLHLDEEWVHTSGLPDTVD
                     YSTGKVDGSGNIYVTTNTISATEKANILTTKYNSSGVVQWSVEKDNADENDYGSAIDI
                     DGSGNVYVGAATWVNGTNYYDYMVIKYNSSGTQQWAVTYNGPGSFYDIPTDIFVDGSG
                     NVY"
     gene            complement(950..1075)
                     /locus_tag="PLL28_11995"
     CDS             complement(950..1075)
                     /locus_tag="PLL28_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70090.1"
                     /translation="MTKLSNPFNYDPFGMITVGRGWEVGSGYRYGFNGQEQDDEV"
     gene            complement(1290..2510)
                     /gene="hutI"
                     /locus_tag="PLL28_12000"
     CDS             complement(1290..2510)
                     /gene="hutI"
                     /locus_tag="PLL28_12000"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNF70091.1"
                     /translation="MASLLFLHIDRLLMADNTLTAVRKGSEMREVPSLDNAWLLLENG
                     VVKGFGGMESAPGHTDEVVDAKGRFVLPAFVDSHTHLVFATGREEEFAMRIAGKTYEE
                     IAAASGGILNSARKLREMPEEALFDAALARLEKLRNQGTGAIEIKSGYGLTTASELKM
                     LRVIRKLKEQSAMPIKSTFLGAHALPDQYKNDKDGYISLIIDEMLPKIATEGLADYID
                     VFCEQGYFTNADTDRILEAGWKFGLKPKIHVNQFTNSGGVQTGIRHKALTVDHLEVMG
                     QAEIDALKASETLPVALPGCSFFINIPYTPARSIIDAGLPLVLASDFNPGSSPSGNMA
                     FVMALACTQMKMTPEEALTAATINGAAAMELEHSTGSISIGKKPGIILTKPMRSLAEI
                     PYYFGDDLVERVFY"
     gene            2574..3656
                     /gene="aroC"
                     /locus_tag="PLL28_12005"
     CDS             2574..3656
                     /gene="aroC"
                     /locus_tag="PLL28_12005"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNF70092.1"
                     /translation="MAGNSFGTLFRITTFGESHGVALGVVIDGCPPGITLDLDFMRNE
                     MQRRKPGQSKLVTQRKEEDEFEILSGVFEGVTTGTPIGMIIRNSDQRSKDYSHLENAY
                     RPSHADYTYDAKYGFRDHRGGGRSSARETAARVLAGSVAKQILLQQHVIIQAYVDAVG
                     DLKLEKPYTELDLSLTETNMVRCPDPDMAEKMINLIAEVKKDGDTIGGTISCVITGTQ
                     PGLGDPVFDKLHADLGKAMLSINAAKAFEYGSGFSGTSMRGSAHNDLFAIDPETKKVI
                     TTTNHSGGIQGGISNGMDIYFRVGFKPVATILKPQQSINVEGETVQVEGKGRHDACVL
                     PRAVPIVEAMAALVMVDHMLIQKSMA"
     gene            3659..4378
                     /locus_tag="PLL28_12010"
     CDS             3659..4378
                     /locus_tag="PLL28_12010"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNF70093.1"
                     /translation="MSHVWFITGTSSGLGKAMAEQVLMHDDTRVYGIGRRHTIVHENY
                     LPINIDLSDLAVLKQFVFPSLEAQTIVLFNNAGRLGEIAYCGNLDNEDIIQSYTVNTI
                     APHLFCNQFIAQYQAADAKKIIINITSGAAKTVYDGWGVYGAGKAALDMFTQSIAAEQ
                     QKINATNKILAYAIAPGVMQTAMQDQIRNTEAVNFSQQQKFKDLYQNNKLYHPEDVAR
                     WFWHFVQSCDSQQETVQRINL"
     gene            4375..6006
                     /locus_tag="PLL28_12015"
     CDS             4375..6006
                     /locus_tag="PLL28_12015"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNF70094.1"
                     /translation="MIPLLFAMLLTAPDEEKLECDWIITNARIYTVDSAFSMVQSMAI
                     DDGYIVATGSNDSVMAHYWSLNVTDMAGKVIYPGFIDGHCHFLHYGVDLSYTNLVGTK
                     SFLDVVELLKKHDITKTSGWIIGRGWDQNDWDYKEFPTREALDKEFPATPVYIERVDG
                     HVALLNGRALELTGITETSSIAGGVVEVKNGKCTGILMDNAMELAKRKMPTKSSKFLT
                     NALARAQSDCFAVGLTSVQDAGLELHEIMTLKTAADNGKLQMRVYAMATAEPKTFDYF
                     MENGPMYGDRLNVRAFKFYADGALGSRGAGLLKPYSDDPDNYGIYYYPYDSLSWQAKR
                     VYNAGFQMCTHAIGDSANRLILDIYGATLKGKNDLRWRVEHCQVVSKSDINKFGQFSI
                     LPSVQPTHATSDMYWATDRLGADRLKTAYAYKDLMKQNGMLIDGSDFPVESINPIYGF
                     YAAVARKDLKMYPDKGFQKENAIGRNEALKAMTIWAAYGAFEEGVKGSLEVNKYADFV
                     ILDKDILAIPEPEIPTAKVLMTVVGGDVVYERKSE"
BASE COUNT         1891 a         1376 c         1487 g         1842 t
ORIGIN      
        1 ataaacgttt cctgatccat caacaaaaat gtcggttgga atatcataaa agcttccggg
       61 cccattgtag gtaactgccc attgttgcgt acccgaagag ttatatttaa taaccatgta
      121 gtcataataa ttagtaccgt tcacccaggt tgcggcacca acgtagacat ttcctgaacc
      181 atcaatgtca attgccgagc cgtaatcatt ttcatctgca ttatcttttt ccactgacca
      241 ttgcacaaca ccagaggaat tatattttgt tgttaaaatg ttcgcttttt cagttgcaga
      301 aatagtattt gtggtaacat aaatatttcc agaaccatca acttttcctg tactataatc
      361 tacggtatcc ggtaaaccag acgtatgcac ccattcttca tcaaggtgta attgactttt
      421 tacattagtt gaaaagaaaa caatccctaa gaggagaata cttctcaaga aaagtaattg
      481 agatttcata aaaattggtt ttagggtaaa agaaatatta ttgaaattta accttcaata
      541 tttggttgcc caaatttttt gtgtaaaatt ttagcaattt cctcaaaatt caccataaat
      601 ggtgaatcct aaaaactgga cacttgttat gaagagtcct ctggatgtca gacggagtct
      661 taatatcact tttggtttca attgttattg atgtgccaat gttagaaaat gatttaccat
      721 ccaaatcaac atatgtatcc atgttactat caatccatct gatgctacca tttgaatcat
      781 cttgaaacca atcatcatta tcgcccttca catcagtact tactataggg ctattgctaa
      841 aaacattgta acatgataaa tctgatttat ttacaggatc cacattccac cttttaccaa
      901 gacgagtatc atgcccccga tactctgcgg tgtttagatt gctatttcct taaacttcat
      961 catcctgctc ttgcccatta aaaccgtatc tgtagccact tccaacctcc caacccctac
     1021 ccacagttat catcccaaac ggatcataat taaatggatt tgaaagcttt gtcatagatg
     1081 gggatggtgt ttgttaaaag taacgattaa aagcttaaaa tgcaaaaaac gtgaagattt
     1141 gaagatgtag tcctacggat ttatttgtgc ttcgtttgta tcgttgtttt catgtacaca
     1201 tccgtttttg gtaataggta actcgttgca agtatgaatt tatcagcaca tggagtgcaa
     1261 tgacatttca cctcagtgga atctgcatat caataaaaca ccctctccac caaatcatcc
     1321 ccaaaataat acggaatctc cgccagcgag cgcatcggct tcgtaaggat gatgccgggt
     1381 tttttgccga tggagatgct tccagtgctg tgttcgagtt ccatggctgc tgctccgttg
     1441 atggtggcgg ctgtaagcgc ttcttccggg gtcattttca tttgcgtgca ggctagtgcc
     1501 attacaaatg ccatattgcc cgatggggaa gaaccgggat tgaaatctga tgcgagaaca
     1561 agtggcaatc ctgcgtcaat gatgcttctg gcaggggtat agggtatgtt tatgaaaaat
     1621 gaacagcccg gaagtgctac aggcaaggtt tctgaagctt tgagggcgtc tatttcggct
     1681 tgtcccatta cttcaaggtg atcaacggtc aatgctttgt gtctgatgcc tgtttgcacg
     1741 ccaccgctgt tggtgaactg gttaacatgg atctttggct tcaatccaaa tttccaacct
     1801 gcctccaaaa tgcggtctgt atctgcattg gtgaagtagc cctgctcaca gaaaacatcg
     1861 atataatcgg ctaagccctc tgtggctatt ttgggcagca tttcgtcgat tatgaggctg
     1921 atgtagccat ctttatcgtt tttatactgg tcagggagtg catgtgcccc aagaaaggtg
     1981 ctttttatgg gcatagcaga ctgctccttc aattttctga tgacacggag catcttgagc
     2041 tcagatgctg ttgtaaggcc gtagccgctc tttatttcga tagcgccggt gccttggttg
     2101 cgaagttttt cgaggcgggc aagggctgca tcgaaaaggg cttcttcggg catttcgcgg
     2161 agttttctgg cgctgttgag gatgcctccg ctggctgcag caatctcttc gtaggttttg
     2221 ccggcaatgc gcatggcaaa ttcctcctcg cgaccggtgg caaataccag atgggtatgg
     2281 gagtcgacaa aagcgggtaa cacaaatcgc cccttggcat ctaccacttc gtcggtatgt
     2341 ccgggtgctg attccatgcc gccaaaccct ttcaccacgc cgttttccaa caacaaccag
     2401 gcattatcca aggacggaac ctcgcgcatt tcgcttcctt tgcgcactgc agtgagggta
     2461 ttgtcggcca taaggagccg gtcgatatgc aaaaacaaca gggacgccat gggacaaaga
     2521 tgcaggaact tgttgaatgc aaaaaattgc tacgatagcg taatttcgcc cctatggcag
     2581 ggaactcttt tggaacgcta ttccgcatca ccacgtttgg cgaatcgcat ggcgtagcgc
     2641 ttggtgttgt gatagacggc tgtccacccg gtatcaccct cgacctcgat tttatgcgca
     2701 acgaaatgca gcgtcgcaaa cccggacaat cgaaactggt tacgcaacgc aaggaggaag
     2761 atgaatttga aatattatcc ggtgtatttg agggtgtcac tacaggtacg ccaattggta
     2821 tgatcatcag aaacagcgac cagcggtcta aagattatag tcacctggaa aacgcctatc
     2881 gccccagtca tgccgattat acctatgatg ccaaatatgg ttttcgcgat catcgcggag
     2941 gcggacgcag cagtgcaaga gaaactgccg cgcgtgtatt ggcaggaagc gttgcaaaac
     3001 aaattctgtt acaacagcat gtgatcattc aagcgtatgt tgatgctgtg ggcgacctca
     3061 aactggagaa accatacact gaactggatc tgtctctaac agaaacaaat atggtgcgtt
     3121 gtcctgatcc ggacatggca gaaaaaatga tcaatctcat agcggaggtg aagaaggatg
     3181 gcgataccat tggcggaact atttcttgtg tcattaccgg aactcagcca ggactcggtg
     3241 accctgtttt tgataaactg catgcagatt taggaaaagc aatgctgagc attaatgccg
     3301 cgaaagcatt tgaatacgga agcggatttt ccggaacaag catgcgcggc agtgctcata
     3361 acgatttatt tgctattgac cccgaaacaa aaaaggtaat tacaactacc aatcattcag
     3421 gtggtattca aggtggcatc agcaatggaa tggacattta tttcagagta ggatttaaac
     3481 ctgtagctac aattttaaaa cctcagcaaa gcattaatgt tgaaggagaa acagtgcagg
     3541 ttgaaggtaa aggtcgccat gatgcatgtg tgttacctcg cgcagtgcca attgtagagg
     3601 cgatggctgc attggtgatg gttgatcata tgcttattca aaaatcaatg gcataaccat
     3661 gtcgcatgtt tggttcataa caggtacttc atccggactt ggaaaagcaa tggccgaaca
     3721 agtattgatg catgacgata cgcgcgttta tggaattggc agaaggcata ctatcgttca
     3781 tgaaaattat ttgccaatca atatcgattt aagtgacctt gcagttttaa agcagtttgt
     3841 atttccaagc ctggaagctc aaactattgt attgtttaat aatgcaggtc gattgggtga
     3901 aatagcttat tgcggaaatt tggataatga ggatattatt caatcctata cagtaaatac
     3961 catcgcccca catttatttt gtaatcaatt tattgcgcaa tatcaggcag ctgatgccaa
     4021 aaaaataatt atcaatatta ccagtggcgc tgcaaaaacg gtatacgatg gatggggcgt
     4081 ttatggagcg ggaaaggcag cattggatat gtttacgcaa agtattgccg ccgagcaaca
     4141 aaaaataaat gcaacaaata aaattcttgc ttatgccatt gcacccggag tgatgcaaac
     4201 ggcaatgcag gatcaaatta gaaatacgga ggctgtgaat ttttcgcaac aacaaaaatt
     4261 caaagacctt tatcaaaaca ataaattgta tcaccctgaa gacgttgcgc gatggttttg
     4321 gcattttgtg caatcatgcg atagtcagca ggaaacagtt caacgcatca acctatgata
     4381 ccattattat tcgccatgtt acttaccgca cccgatgaag aaaaactcga gtgcgactgg
     4441 atcattacca atgccaggat ttatacggta gatagtgcat tttccatggt gcaatctatg
     4501 gctatcgacg acggatatat agtagccaca ggatctaacg attctgtaat ggcgcattat
     4561 tggtcgctta atgtaacgga tatggcaggt aaggttattt atcccggatt tatcgacggc
     4621 cattgtcatt ttctgcatta tggagtcgat ttatcctata ccaatctcgt aggcacaaaa
     4681 tcatttctcg atgtcgttga attattgaag aagcacgata ttaccaaaac aagcggatgg
     4741 ataataggaa ggggatggga tcaaaatgat tgggattaca aagaatttcc aacgcgagaa
     4801 gcgctggata aagaatttcc cgcaactcct gtttatatcg agcgtgttga tggtcatgtt
     4861 gcgttattaa atggtcgtgc attggaactt acaggtatca cagaaacgag ttcaattgcc
     4921 ggtggggtag tggaagtgaa aaatgggaaa tgcacgggta tattaatgga taacgcgatg
     4981 gaactcgcta agcgaaaaat gccaaccaag agttctaaat ttctgactaa tgcattagcg
     5041 cgtgcacaaa gcgattgttt tgctgtcggc ctcaccagcg tgcaggatgc cggcctcgaa
     5101 ttgcacgaga ttatgacttt gaaaactgcc gcggataatg gaaagttgca aatgcgtgtg
     5161 tatgcgatgg caaccgcaga gcccaaaaca ttcgattatt ttatggaaaa cggtcccatg
     5221 tatggcgatc ggttaaatgt gcgcgcattt aaattttatg ctgatggcgc acttggcagc
     5281 agaggtgcag gactcttaaa accatatagc gatgatccgg ataattatgg tatttattat
     5341 tacccttacg attctttaag ctggcaggca aaacgcgttt ataatgccgg attccaaatg
     5401 tgtacgcatg ccattggcga ttcagctaat agacttatcc tcgatattta tggcgcaaca
     5461 ttaaaaggaa aaaatgatct gcgttggcga gtggaacatt gtcaggtagt tagcaaatct
     5521 gacattaata aattcggaca attttccatt cttccatctg ttcaacccac acatgctacc
     5581 agtgatatgt attgggcaac agacagactt ggtgcagata gactaaaaac ggcatatgca
     5641 tacaaagacc tcatgaagca aaatggtatg ttgatcgatg gcagcgattt tccggtagaa
     5701 tcaataaacc cgatttatgg attttatgca gctgttgcca gaaaagattt gaaaatgtat
     5761 cccgataaag gttttcaaaa agaaaatgcc atcggccgaa atgaagcatt aaaagcaatg
     5821 accatttggg cagcatacgg cgcatttgaa gaaggtgtga agggaagtct tgaagtaaat
     5881 aaatacgccg atttcgtaat ccttgataaa gacatcctgg ccatccccga acccgaaatt
     5941 cctacagcga aagtgttgat gacagttgtg ggaggtgatg ttgtctacga aagaaagtct
     6001 gagtgagtgg gtgagcttgc ctcgccgata ggcgggtggg tgagtggtga gtgggcacta
     6061 tgcaaattcg ctcgctttgc gagcgaatgg ttgcattagt ctcactactc aatacccact
     6121 actcactacc ggtcgtgaat cgtgaatcgt gactcgtgag tgtggtgagt acctaagaat
     6181 cgagaatgtg atgcgagttt gcatttacct gatacttgat acttgatacc tgatacctga
     6241 taccagattc caagtctaga tactagatac ttggttctag atactaatgt cgtgaatcgt
     6301 gactcgtgaa tgccaacttt gcgactttgc atctttgcgt cttagcgcga accccgcctc
     6361 accccctgac ctcctctcca ctcctggcgt cgcagagagg gggaattaaa tgcatttgat
     6421 gatgaattat tcttaaggtt ttaattaaag gttatgaaaa ttgcaatttt aaaggagttg
     6481 aaaacgctat tgattcccac attctcacat tctcacccgc ctgcggcgag gctggcattc
     6541 ccacattccc acattcccac ccgcctgcgg cgaggctggc attcccacat tcccac
//
LOCUS       DAOQHY010000169         6477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_6440_length_6477_cov_5.858144, whole genome shotgun sequence.
ACCESSION   DAOQHY010000169 DAOQHY010000000
VERSION     DAOQHY010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6477
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6440_length_6477_cov_5.858144"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PLL28_12020"
     CDS             <1..447
                     /locus_tag="PLL28_12020"
                     /inference="COORDINATES: protein motif:HMM:NF013821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HNF70095.1"
                     /translation="SSKIRAFLLEGIIDSANELLGYNYALTGPVVKGDQIGRTLGFPT
                     ANIFVEDSYKLIPANGVYVVRVVMKQHPGEYFGLLSIGTRPTFNKTESRVEVHILDFN
                     ESIYGETITIQFLHFLRKDKKFDSATSLVEAMHADKQQALTYLASL"
     gene            complement(463..1473)
                     /gene="holA"
                     /locus_tag="PLL28_12025"
     CDS             complement(463..1473)
                     /gene="holA"
                     /locus_tag="PLL28_12025"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNF70096.1"
                     /translation="MDYRDLINDIKARKFKPVYLLHGDEAYYIDKIAEYMEDNILPPD
                     QKDFNFSVFYGKDTHPQTVIDTSMRFPMFSDFNVVMLKEAQMMKGKGNEIDKLETYIE
                     KASPTTILIICYKEGRFDARKRMFKLIKEKGVIFESETIKESELPMFIESYLKRKKAS
                     IDSAALRLLVQYLGADLAKMSNELDKLCINLPESGRISVDQIEKNIGISKDYNVYELQ
                     SALLTKNFALVYTIVSYINDNQKANPFIFTISNIHAAFQRLYHYLLGGEVSDFDMYKV
                     YQIHSSQKNEYKAARRLYTVQRVEEIFELLLDYDLRSKGLNNGETEHDELLREMVYKI
                     MS"
     gene            1501..1953
                     /locus_tag="PLL28_12030"
     CDS             1501..1953
                     /locus_tag="PLL28_12030"
                     /inference="COORDINATES: protein motif:HMM:NF016220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HNF70097.1"
                     /translation="MSLQFSTYDLRVQESESGRRIYDIIRKKYVVLTPEEWVRQHIIH
                     YLITDHGFPKSLISVEKQLTVNTLTRRTDIVLYNHAGKPVLIIECKASDIKLSQAAVD
                     QAARYNLTLQVPWLWVTNGVSHVWCHISHEEGNWRVEGHLPPVSELLR"
     gene            2037..3374
                     /gene="gdhA"
                     /locus_tag="PLL28_12035"
                     /pseudo
     CDS             2037..3374
                     /gene="gdhA"
                     /locus_tag="PLL28_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585547.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
     gene            complement(3485..4192)
                     /locus_tag="PLL28_12040"
     CDS             complement(3485..4192)
                     /locus_tag="PLL28_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF70098.1"
                     /translation="MDSRKPKILYVEDDVNLGFVTKDNLLKRGYDITLCEDGLTASHT
                     FQKQKFDLVILDVMLPKIDGFALAQKLRDKDKNVPIIFLTAKSMLEDKLQGFKLGADD
                     YITKPFSIDELTHKIEVFLKRSKVFAESPEAESKNIYHIGTYTFDFKELKLFNTTQQH
                     TLTIREAELLRMLSIQRNQVVRREAILHQVWGEDNYYIGRSLDVFISRLRKLFADDPG
                     VEIENVRGVGFKFNVAD"
     gene            complement(4202..5464)
                     /locus_tag="PLL28_12045"
     CDS             complement(4202..5464)
                     /locus_tag="PLL28_12045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNF70099.1"
                     /translation="MNTTTIRWVIALGIVAVVTIILSQVFWIRKGLIINQSNFENAVT
                     VTLEQIAAKIEIANHGKKVTAHPVVRISPHAYKVNIDEPIDLNQLDYFIRSEFANPFH
                     KVDFIYQVFESTRDDLVFEETVSANSSESTIVFPTNLPKLMESTYYFKINFPERPIVN
                     SVMLFVWATSIIAMAVVIAFFWYSLDVIFRQQRLSKFQKNFINNLAHEFKTPISTIGI
                     SADVLLEPEIAQEPDRLKGYANIIRNENLRLTGQVSKILELAKLEDDELLLNLETFHI
                     NPLIEEIVQSFTVRVEEKSGGIVVDYTTKQDLVTADRVHLQNVVNTILDNALKYNDKI
                     PEITITVGRDQQGLFINIGDNGIGIPKEFHRRIFEKFFRVPTGNVHNVKGYGLGLNYV
                     KMIAKSHGWHLRVDSQIGKGSIFTVIIP"
     gene            complement(5512..6180)
                     /locus_tag="PLL28_12050"
     CDS             complement(5512..6180)
                     /locus_tag="PLL28_12050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF70100.1"
                     /translation="MSKQVHILIAETQYLIIEGLQSIFRRYPEFSVLGIMVNKQDLFD
                     NLHVLKPDVVVIDPLSLEDFRVDDLQTIETVCPGARALIITDISDPNIVHRIVDTGAK
                     GYLTKTCDEKEIVDAIYALLRGEQMYCHKVVNILVDGRTVEVDNCEPTGLSEREVEII
                     RLIANGYTTRQIADTLYRSFHTITTHRKNIMKKLGINSTSELMVYAMNTGLIQPREQL
                     VAGK"
     gene            complement(6276..>6477)
                     /locus_tag="PLL28_12055"
     CDS             complement(6276..>6477)
                     /locus_tag="PLL28_12055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70101.1"
                     /translation="CKIDNTWPTDASITVYDLAGNFMQIAVGQAGLVRYADVSSLPEG
                     VYLLRIQAANRVGSGSFVVARH"
BASE COUNT         1936 a         1365 c         1314 g         1862 t
ORIGIN      
        1 tcttcaaaaa ttcgtgcatt tttactcgaa ggtattattg attccgccaa tgaattattg
       61 ggttataatt atgccctcac cggtccggtg gtaaagggag atcagatcgg cagaacactc
      121 ggattcccaa ccgctaatat atttgttgaa gacagctata aactcattcc ggcaaatggc
      181 gtttatgtgg tgcgtgtagt gatgaaacaa catcccggag agtactttgg attattaagc
      241 attggcaccc gaccaacatt caataaaaca gaatcgcgcg ttgaagtaca tattctcgac
      301 ttcaatgaat ccatttatgg ggaaacgatt accattcaat ttttgcattt tttgcggaag
      361 gataaaaaat ttgattccgc cacatccttg gttgaagcca tgcatgcaga taaacaacag
      421 gcactcacct atcttgcatc tctttaatta attcaggaat tttcacgaca tgattttgta
      481 aaccatttca cgcaataatt cgtcgtgctc ggtttcgccg ttgtttagtc cttttgagcg
      541 gaggtcgtaa tccaataata attcaaaaat ctcttccacg cgctgcaccg tatacaatct
      601 gcgcgcagct ttatattcat tcttctgcga actgtgtatt tgataaacct tgtacatatc
      661 aaaatcactc acttcaccac caagcaaata atgatatagg cgctgaaagg ccgcatgaat
      721 attagatatc gtgaaaataa aaggattagc cttttgattg tcattgatat aagatactat
      781 tgtataaacc agagcgaagt tttttgtaag caacgcactt tgcaattcat atacattata
      841 atccttgcta atacctatat ttttttcaat ctgatcgaca gaaatccttc cgctttccgg
      901 aagattgata caaagcttat cgagctcatt cgacattttg gcaagatctg caccaagata
      961 ttgcactaac aaccgcaaag cagctgaatc aatggaagct tttttccgtt tgaggtatga
     1021 ctcaatgaac atcggaagct cagattcttt gatcgtttca gactcaaata tgacaccttt
     1081 ctctttaatg agtttgaaca tgcgtttccg cgcatcaaat cggccctctt tataacagat
     1141 gattaaaatg gttgttggtg atgccttttc aatataagtt tccagcttat caatttcgtt
     1201 tccctttcct ttcatcattt gtgcttcttt cagcataacg acattaaagt cagaaaacat
     1261 tggaaagcgc atgctggtgt ctatcactgt ttgaggatgc gtatcctttc cataaaaaac
     1321 actgaaattg aaatcctttt gatcaggagg aagaatatta tcctccatat attcagcgat
     1381 cttatcgata taataagcct catcgccatg caataaatac acgggcttga atttgcgggc
     1441 ttttatatca ttgatcagat cgcgatagtc catggcacaa aaataactta attttacagg
     1501 gtgtcattgc aattctccac atatgatctg cgtgtgcagg aatcagaaag cggcaggcgc
     1561 atctatgata tcattcgaaa aaaatatgtt gtccttactc ctgaagaatg ggttaggcaa
     1621 catatcatac attatctcat caccgatcac ggctttccaa aatcactcat cagcgtcgaa
     1681 aagcaactaa cggtaaatac tctcactcgt cgcaccgata ttgtattgta taatcacgcc
     1741 ggaaaacctg ttttgattat agagtgcaaa gcatcagaca ttaaactttc acaggcagca
     1801 gttgaccagg ctgcgcgata taatctgaca ctacaggtgc cctggttgtg ggttaccaat
     1861 ggcgtgagtc atgtgtggtg tcatatctct catgaggaag gaaactggcg tgtggagggc
     1921 catttacccc ccgtttcgga acttttgagg tgacaggtca ctaaaaaagg tgatttaatc
     1981 catttggaac tatcaggatt tctcctagct tcacaccaca aaaaaccact taacctatgg
     2041 aaaagaacgt taaagcattc atgaccctgg tagaatccag aaacccggga gaaaaagagt
     2101 tcttacaagc agttttcgaa gttgccgagg ccgttatccc atttatagag gacaacccca
     2161 agtacaagga agcaaaaatt ctggaacgca tagccgaacc tgagcgcgtg atgatgtttc
     2221 gcgtaccgtg ggttgatgac tagggcaatg tgcaagtaaa tcgcggttac cgcattcaaa
     2281 tgaacagtgc catcggaccg tacaagggcg gattgcgctt tcacccttct gttaacctgt
     2341 cgatcctgaa attcctcgct tttgagcagg tattcaagaa tagccttacg acactaccga
     2401 tgggtggtgg aaaaggtgga tcgaattttg atcctaaagg aaagagcgat gctgaagtaa
     2461 tgcgcttctg ccaaagcttt atgatggagt tgaaccgtca tatcggtgca aataccgacg
     2521 ttcctgcagg tgatatcggt gtgggcggac gagaaatcgg atacctcttt gggatgtata
     2581 aaaagttgcg caatgaattt acgggagtac ttaccggtaa aggattggaa tggggcggca
     2641 gtttaattcg ccctgaagca acaggatatg gttgcgttta tttcgctcag gaaatgatgg
     2701 gcacacgcaa agcatcattc aaaggcaaaa cagtaaccgt atcaggaagc ggaaacgtag
     2761 cacaatatgc catcgaaaaa tgtctcgaac tcggagcaaa agttgttacc gccagcgaca
     2821 gtgacggata catctacgat aaaaatggct tcacaacaga aaaacttgct atgctgatgg
     2881 atatcaagaa taacatgcgc ggacgcatta aagattacgc cgacaaagtg aaatgccaat
     2941 acattgccgg taaaacacct tgggaagtaa aatgtgatat cgacctgcct tgcgctacgc
     3001 aaaatgagct caacggcaag gatgcaaaag tgctgattaa aaatggtgtg gtatgtgttg
     3061 cagagggtgc gaatatgcct tgtactcctg aagcaattac cgctttcctg gaaaataaaa
     3121 tcttatatgc tccgggtaaa gcaagtaatg ccggtggtgt agctaccagt ggtcttgaaa
     3181 tgagtcaaaa cagtttgcgc atgaactgga caagagagga agtagataat cacctcaaaa
     3241 acatcatgca caacatccac aagacctgcg ttacttacgg taaagaagga aaatacatca
     3301 actatgtgaa aggcgcaaat atcggaggct tcgtaaaagt agccgattca atgctggcac
     3361 aaggtctggt gtaaattgat atgattttat taaaaaggag tttcgaaaga gactcctttt
     3421 tttatgcatt aatgaggagg aaaaaaaata atcagtgcct taatgattga tcctttaaat
     3481 caacttagtc ggctacatta aatttgaacc ctactcccct tacgttttcg atttccactc
     3541 cagggtcatc tgcaaataat ttcctgaggc gtgaaataaa tacatcaagc gacctgccga
     3601 tataataatt gtcttcaccc catacctgat gcaaaattgc ctcgcggcga actacctgat
     3661 ttcgttgaat agaaagcatg cgtaatagtt cagcttcgcg aatggtgagt gtatgttgct
     3721 gtgtggtatt aaagagtttc aattccttga aatcgaaagt ataagtgccg atgtgataaa
     3781 tatttttcga ttcagcttcc ggactttcag caaacacttt gctgcgtttc aaaaatactt
     3841 ctatcttatg tgtaagctca tcgatgctga atggttttgt gatataatca tcagcaccga
     3901 gcttaaaacc ttgcaattta tcttcaagca tggattttgc tgtcagaaaa atgataggaa
     3961 cattcttgtc cttatcgcgc aatttctgcg caagcgcaaa tccatcgatc ttgggtaaca
     4021 tcacgtccaa aataaccagg tcgaattttt gcttttgaaa ggtatgtgat gccgttaatc
     4081 catcttcaca taatgtaatg tcataccctc ttttgagcaa attatctttt gttacgaagc
     4141 cgagattgac atcatcttct acatataata ttttgggttt tctgctatcc atgtgtctga
     4201 gttagggaat gatgactgta aatatactgc ctttgcctat ttgtgaatcc acacgcaagt
     4261 gccatccatg tgatttagct atcattttaa cataattcag cccgagtccg tagcctttca
     4321 cattgtggac atttcctgta ggcaccctaa agaatttctc gaaaatgcgt ctgtgaaact
     4381 ctttcgggat gccaattcca ttatcaccga tattaataaa tagtccctgc tgatcgcgac
     4441 caacagtaat ggtgatttcc ggaatcttat cattgtactt caacgcattg tcgagtatcg
     4501 tgttcacaac attttgcaaa tgcactcggt cggcggttac cagatcttgt ttggtagtgt
     4561 aatcaacaac aataccaccg cttttttctt ctacgcgaac cgtgaatgat tgcacaattt
     4621 cttctattaa tggattgata tgaaaagttt caagattgag caacaattca tcatcctcca
     4681 atttcgccaa ttcaagaatt ttactcacct ggcccgtaag ccgtaaattt tcattgcgaa
     4741 tgatattggc atatccctta agtctgtcgg gctcttgcgc aatctccggt tctaataaaa
     4801 catctgccga aataccaatc gtgctgatag gtgttttgaa ttcatgagcc agattgttga
     4861 tgaagttttt ctggaattta cttaatcgct gttgtcggaa gatgacatcc agcgaatacc
     4921 agaaaaacgc tataacaacc gccatcgcta tgatagacgt ggcccagaca aataacatta
     4981 ctgaattcac aatcggacgc tcagggaagt tgatcttaaa atagtaagtc gactccatta
     5041 actttggtaa attagtaggg aatacgatag tactttcact cgaattggca gatactgttt
     5101 cttcaaaaac caaatcgtcg cgcgtggatt caaacacctg ataaatgaaa tcgactttat
     5161 gaaatggatt tgcaaattca ctacgaataa aataatccag ttgattcaag tctataggtt
     5221 catcgatatt tactttgtaa gcatgtggtg aaatccgcac aacaggatgt gccgttacct
     5281 tttttccgtg attggcgatt tcaatttttg cagctatctg ttccaaggta acggtaactg
     5341 cattttcaaa attgctctga ttaataatca agcccttgcg tatccagaat acctgcgaaa
     5401 ggataatcgt aacaacagcc acgataccta atgcaattac ccaacggata gtagtagtat
     5461 tcataaatca gtgctaaaga taaggagttg aaccctccct gtgtaagacg gtcatttacc
     5521 tgcaacaagt tgctcacgcg gttgaataag tccggtattc atggcataca ccatcaattc
     5581 actggtgctg ttgatgccga gcttcttcat gatgtttttg cgatgcgtgg tgatggtatg
     5641 aaaactgcga tacagcgtat ccgcgatttg gcgcgtcgta tagccatttg caataagtct
     5701 gatgatctct acttcgcgtt ctgataatcc ggttggctcg caattatcta cttcaactgt
     5761 tctaccatct actagaatat tcaccacttt gtgacaatac atctgctcgc ctcgcaaaag
     5821 cgcataaata gcatcgacaa tttctttttc atcacaagtc ttggtcagat agcctttagc
     5881 accggtatcc actatgcggt gcacaatatt cggatcgctg atgtcggtga tgatcaatgc
     5941 acgcgctccc ggacaaacgg tttcgatagt ctgcagatca tctacacgaa aatcttcaag
     6001 actgagcgga tcaataacaa caacatcagg ttttaacaca tgcagattat cgaataagtc
     6061 ctgcttgttc accataatac ccaacaccga aaattccgga taacgacgga atatcgattg
     6121 caagccctct atgataaggt attgcgtttc ggctatgaga atgtggacct gcttggacat
     6181 tactatttag actaattcta aacataaaga tacgtctttt gttcaaaatc acaagcatac
     6241 acgcatcatc ctgatcgtgc tgaccgggga ggagatcaat gccgggcgac cacgaaggag
     6301 cctgagccta cgcggtttgc agcttgaata cggagcaggt aaacgccttc cggaagactg
     6361 ctgacatcgg cataacgcac taaaccggcc tgacctacag caatctgcat aaaattgcct
     6421 gccaaatcgt aaacagtgat tgaagcatcg gtgggccagg tattgtcaat tttgcat
//
LOCUS       DAOQHY010000170         6460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_6461_length_6460_cov_6.048087, whole genome shotgun sequence.
ACCESSION   DAOQHY010000170 DAOQHY010000000
VERSION     DAOQHY010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6460
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6461_length_6460_cov_6.048087"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3600)
                     /gene="metH"
                     /locus_tag="PLL28_12060"
     CDS             complement(<1..3600)
                     /gene="metH"
                     /locus_tag="PLL28_12060"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF007024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNF70102.1"
                     /translation="MEQKLRKILKERILVLDGAMGSMIQRYKLSEADFRGERFATHPH
                     DLKGNNDLLSITKPEVIREIHRQYLEAGADIIETNTFGATAIAQADYHLESLGYELNK
                     VSAEIARLEADAFTALNPEKPRFVAGAIGPTNKTLSLSPDVNNPGFRSVYFDEMAQAY
                     YDAARGLMDGGAHILIIETIFDTLNAKAALYAIDKLFNETGMSLPIMVSGTITDASGR
                     TLSGQTLEAFVISMSHLPLLSIGLNCALGAKQLKEHIEILSRETDLFVSAYPNAGLPN
                     AFGEYDETPESNGNDVEVYLQHGWVNIIGGCCGTTPDHIRYIAEKAKKYPPRVPPTLP
                     RLPKFSGLEKLVIYPESNFLNVGERTNITGSKQFKRLITEEKYDEALVVARQQVENGA
                     QMIDVNMDEGMLDSVEVMRTFLNLIAAEPDISKVPVMIDSSKWEVIEAGLKCIQGKGV
                     VNSISLKEGEEAFKHHAREARRLGAAVIVMAFDETGQATSFEHRIAICARAYKILTEE
                     VHFSPTDIIFDPNILTVGTGIEEHNNYAVDFIKATAWIKENLPGALVSGGVSNISFAF
                     QGNNTVREAMHSAFLYHAIKAGMDMGIVNAGMIDIYENIDAALLQHIEDVLLNRHPDA
                     TEKLISFAEQLKQKDKTAIQTDTWRSLPLEARIQHALVKGIADHIEADMEEALPAYPG
                     PLEIIEGPLMAGMQVVGDLFGSGKMFLPQVVKSARVMKKAVAYLQPYIEQTKLQNNQS
                     SNRKKILLATVKGDVHDIGKNIVGVVLACNNYDIIDLGVMVPAEKILQTAVQEHADII
                     GLSGLITPSLDEMVHVAQLMQERHMSTPLLIGGATTSRAHTAVKIAPAYQQAVVHVTD
                     ASRSVTIAGKLLGGEKAAFVKEIADEYAIVQQQHAARQSFKGYLTLAEARNNAFTLSE
                     AAHYPYQPVQPGIHVLDIAIDTLIPYIDWTPFFTTWELHGKYPMIFEDAVIGEEAKKL
                     FDDAQEMLQQIAHEKWLQSKAVIGLFACNRDGDDIVIEETQTRLHHLRQQNKKADGQP
                     NYCLSDFVLSANSGVKDYMGAFAVTAGIGIETKLAEFNAAHDDYKSILLKALADRLAE
                     ACAEYMHQQVRTKYWGYAANEQLTNEALISEQYQGIRPAPGYPACPDHTEKVSLFNLL
                     DVTKHTGVQLTESMAMYPAASVSGWYFAHPDAKYFGVGKIGKDQVEDLAKRKGK"
     gene            4095..6290
                     /locus_tag="PLL28_12065"
     CDS             4095..6290
                     /locus_tag="PLL28_12065"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HNF70103.1"
                     /translation="MLKKSLLSTAFAFVLGGLVMAQAPSRSCATMDVDARLRAEDPTY
                     AANREAIEAFTQNYIATEGERAVVTIPVVVHVVYNVAAENISDTRIFEQIDVLTKDFR
                     RLNADVGETPAYFAGVAADCEVSFCLATVDPSGNPTTGITRTSTTKTSFSTADDMKFA
                     TYGHVAWDRNKYLNLWVCDLGSGLLGYAQFPGGAAATDGVVIDYAYFGVTGAGAPYNE
                     GRTATHEVGHWLNLYHIWGDDGTGCGGSDSVADTPNQADENYGCPGGTIRISCSNGPN
                     GDMYQNYMDYTDDGCMNLFTAGQKTRIQALFAVGGSRVSLTTSNGCGAAATCSTPTGM
                     STTSIGTTSATFNWTAVAGASSYNVQYRIVGAATWTSTTSATNSKAVTGLTAGSNYEW
                     QVQTVCAGGTSSWTASTNFSTTGGATCAVPTGMNTTSIGTTSATFNWSAAAGATSYNV
                     RYKATAAATWTTTTSATTSKAVTGLTAGTAYEWQVQTVCSGGTTSAYTASTNFTTTGG
                     ATCVDNYEPNNSRGTAALVSTGVTYGELIATSTDIDWFKFTTTSPNTKIKVNMTSLPA
                     DYDIRLYDNAGTQKGSSINGGTTDEQIIWNSTSAGTRYIKINGYAGAFNASDCYDFLI
                     SVASTNWKTDEAFADVAGKEWENTIMGIYPNPVTGSVVTVDYFSVLEDMDVTVQVFDV
                     LGRMVSSSVVGIVNGPNSIDLNVADLQSGMYFVTISSAKFMQSQNFIVE"
BASE COUNT         1684 a         1517 c         1307 g         1952 t
ORIGIN      
        1 tttccccttc cgcttggcca gatcctcaac ctgatctttg ccaatttttc ccacaccaaa
       61 atacttcgca tccggatgcg caaaatacca tccgctcaca ctcgctgcag gatacatagc
      121 catgctttct gtcaattgaa ctccggtgtg tttggttaca tccaacaaat taaacaagct
      181 aaccttctcc gtatgatccg gacaggcagg ataaccgggg gcaggacgta tgccctgata
      241 ttgttcgctg atgagcgcct cattagttaa ttgctcattc gctgcatatc cccaatactt
      301 agtgcgcacc tgctgatgca tatattctgc acatgcttcc gccaacctgt cggctaatgc
      361 ttttaataaa atgcttttgt aatcatcatg tgcagcgtta aattcagcca gcttcgtttc
      421 aattccaata cctgctgtaa cggcaaatgc acccatgtaa tcttttacgc cgctgtttgc
      481 acttaataca aaatcactca aacaataatt tggctgacca tcggcttttt tattctgctg
      541 tcgtaggtgg tgcaatctgg tttgtgtttc ttcgatcaca atatcatcac catctctatt
      601 acatgcaaat aatccgatca ctgctttcga ttgcaaccat ttttcatgcg cgatctgttg
      661 caacatttcc tgtgcatcat caaacaattt cttcgcttcc tcgccaataa ccgcatcttc
      721 aaatatcata ggatattttc catgcaattc ccatgttgta aaaaatgggg tccagtcgat
      781 atacgggatc agcgtatcaa ttgcaatatc caaaacatgt ataccgggct gaacaggctg
      841 ataaggataa tgcgctgctt cagataaagt aaatgcatta ttccgtgctt ctgctaaagt
      901 taaatatcct ttaaatgatt gtcgcgctgc atgttgctgc tgcacgatcg catactcatc
      961 agcgatctct ttaacgaatg cagctttttc tccgcccaat aatttccccg caatcgtaac
     1021 actgcgtgaa gcatcggtta catgcacaac agcttgctgg tatgccggag caatttttac
     1081 agccgtatgt gcccgcgaag tagtggcacc accaatcaat aatggtgttg acatatgccg
     1141 ctcctgcatc agttgcgcta catgcaccat ctcatctaat gatggtgtaa ttaatccgct
     1201 caatcctatg atgtctgcat gctcctgcac tgctgtctgc aatattttct cggcaggcac
     1261 catcaccccc aggtcgataa tgtcgtaatt gttacaagcc aagacaacac ccacaatatt
     1321 ttttccgata tcatgcacat cacctttcac cgttgccagc aatattttct ttctgttgct
     1381 gctttgattg ttttgcaatt ttgtttgttc aatatatggc tgcaaatagg ctactgcttt
     1441 tttcattaca cgcgcgcttt tcaccacctg cggcagaaac atttttccgc tgccaaaaag
     1501 atcgcctact acctgcatgc cggccatgag cggaccttca atgatctcca gtggtccggg
     1561 atatgcgggc aatgcctctt ccatatctgc ttcaatatga tcggcgatgc ctttcactaa
     1621 ggcgtgttgt atgcgtgctt caaggggcaa gctgcgccag gtatctgttt gaattgctgt
     1681 tttatctttt tgctttaact gttctgcaaa gctgatcagt ttttctgttg catccggatg
     1741 gcgattcaat aatacatctt caatatgttg taataatgca gcatcaatat tttcataaat
     1801 atctatcatg cctgcattca caatgcccat atccatacct gctttgatgg cgtggtataa
     1861 aaatgcgctg tgcatggctt cgcgaacggt attattgccc tgaaatgcaa aagatatatt
     1921 acttacgcca ccgctcacta aagcacccgg cagattttct ttgatccatg cggtcgcttt
     1981 aataaaatca acagcataat tattgtgttc ttcaatgcct gtgcctaccg tcaaaatatt
     2041 cggatcgaaa atgatatcgg taggggagaa gtgtacttct tctgtcagta ttttataggc
     2101 acgtgcacaa atagcaatgc gatgttcgaa gctggtcgcc tgacctgttt catcaaatgc
     2161 catcacgatc actgctgcac ctaatctgcg tgcctcgcgt gcatgatgtt taaatgcttc
     2221 ctcaccttct ttcagagaaa tggaatttac tacccctttt ccctgaatac attttaatcc
     2281 cgcttcgatc acttcccatt tactggaatc tatcatcacc ggcactttcg aaatatccgg
     2341 ttctgcagcg atgagattca ggaaagtgcg cattacttca acagagtcga gcatcccttc
     2401 atccatattc acatcgatca tctgcgcgcc attttctact tgttgacgcg caaccactaa
     2461 tgcttcatca tatttctctt ccgttattaa tcgcttgaat tgtttgctac ctgtaatatt
     2521 ggtgcgctca cccacattga ggaaattgct ttcaggatag atcactaatt tttccagacc
     2581 tgaaaatttc ggtaatcgtg gaagtgtggg cggtacgcgc ggcggatatt tttttgcttt
     2641 ttctgcgata tagcgaatat gatcaggcgt ggtgccgcaa catccgccaa taatatttac
     2701 ccagccatgt tgcagatata cttctacatc gttgccattg ctttcaggtg tttcatcata
     2761 ttcaccaaaa gcattaggta aacctgcatt cggataagca gatacgaaca aatctgtttc
     2821 gcgggataat atttcgatat gttcttttaa ttgtttcgca ccgagtgcac aattcaaccc
     2881 gatactcaac aaaggcaaat gactcataga aataacaaat gcttcgagtg tttgtccgct
     2941 caacgtgcga ccactggcat ctgttatggt tccgcttacc attattggaa gcgacattcc
     3001 tgtttcgtta aataatttat cgatagcata taatgcagct tttgcattta atgtatcgaa
     3061 aatagtttca atgatgagga tatgtgcacc gccatccatt aatccgcgtg cggcatcata
     3121 atacgcttgt gccatttcat cgaaatacac cgagcggaac ccgggattat tgacatccgg
     3181 tgaaaggctg agcgttttat tggtgggtcc gatagctccg gcaacaaacc gcggtttttc
     3241 aggattgagg gctgtaaatg catctgcctc cagtctggca atttcggcac ttactttatt
     3301 gagctcatat cccagggatt ccaggtgata atcagcctga gcgattgcag tagccccaaa
     3361 agtgttggtt tcaataatat cggctcctgc ttcaaggtat tggcgatgta tctcgcggat
     3421 cacctcgggt ttggtaatgg acagcaggtc gttattccct ttgaggtcgt gcggatgagt
     3481 ggcaaatcgt tcgcccctga aatcggcctc cgacagctta tagcgctgaa tcatgctccc
     3541 catagcgcca tcaagcacta aaatgcgttc cttgagtatc ttcctaagct tttgttccac
     3601 agcgcaaaaa taggtgaaca cagattagag ccgtttagtt ttgagaaaat tgacaattca
     3661 atcgcaaacg gaacgtttgg ttgcatttta tgcaaaaccg aaacataagg ctttcagcga
     3721 tttgaagact ttgtaaatac tgctgcattt tggctccggc gtgacaggca aaaaaaatta
     3781 tttataaact aaattcattg ataatcaatt aattagacaa ttaaatgact gaaatttcga
     3841 cacatttaag cattcctgtg acattgaaca gttccttgtt tataaaaagg aagaaaaaaa
     3901 cttgttggaa ttaaaaatct tgtaacttta cgcaactttt ttgggggagg gggcgtcttg
     3961 ttcttcgata tagcaatata ttgattttgt gctcatcgcg tttttctcct ccgacgtgag
     4021 cctagaaaca ccacacacca aacagtatta tttatttaac gtattgattt attaacccta
     4081 actattcatt tcacatgttg aaaaaatcgt tactttctac ggccttcgct tttgtgcttg
     4141 gaggtctggt gatggctcag gctccatcaa gaagctgcgc cactatggat gtggatgcac
     4201 gccttcgtgc tgaagaccca acctacgctg caaatcgcga agcgatcgaa gcatttactc
     4261 aaaactacat tgccaccgaa ggtgagcgcg ctgtagttac tatccctgtt gtagtacacg
     4321 ttgtttataa cgttgctgct gaaaacattt ctgacacacg cattttcgaa caaattgatg
     4381 tattgacaaa agacttccgt cgcctcaatg ctgacgttgg tgaaactcct gcctattttg
     4441 ccggtgttgc tgccgattgc gaagtaagct tttgtcttgc tactgtagac ccaagtggta
     4501 accctactac cggtattaca cgcactagca ctaccaaaac ttcattcagc actgctgatg
     4561 atatgaaatt tgctacttac ggacacgttg cttgggatcg caacaaatac ctcaacttgt
     4621 gggtatgcga cctcggaagc ggtttgttag gttacgctca gttccctggt ggtgctgctg
     4681 ctactgacgg tgtagttatt gactacgctt acttcggtgt tacaggtgca ggtgctcctt
     4741 ataacgaagg tcgtactgct acccacgaag ttggtcactg gttaaatctt taccacattt
     4801 ggggtgacga tggtaccggt tgcggtggtt cagactcagt tgccgatact ccaaaccaag
     4861 ctgatgagaa ctatggctgt cccggtggaa ctatccgtat ctcttgctct aacggtccta
     4921 acggcgacat gtatcaaaac tacatggact acactgacga tggttgtatg aacctcttca
     4981 ctgcaggaca aaaaactcgt atccaggcat tgtttgctgt tggtggttca cgcgtgagcc
     5041 tcacaacttc taatggttgc ggtgccgcgg ctacttgtag cactcctaca ggcatgtcta
     5101 ccacatcaat tggtactact tcagctacct tcaactggac tgctgttgcc ggagcttcta
     5161 gctacaatgt acaatatcgt atcgttggtg ctgccacttg gacttctaca acttcagcta
     5221 caaactctaa agctgtaaca ggtcttactg caggttctaa ctacgaatgg caagtacaaa
     5281 cagtttgtgc aggtggcacc agctcttgga ctgcttctac aaacttctca actacaggtg
     5341 gtgcaacttg tgctgttcca accggtatga atactacttc tattggcact acttcagcta
     5401 cctttaactg gtctgctgcg gcaggtgcta ccagctataa tgttcgctat aaagcaactg
     5461 cagctgctac ctggactacg actacttcag ctaccacttc taaagctgtt accggcctca
     5521 ctgcaggaac tgcttacgaa tggcaagtac aaactgtatg tagcggtgga acaacttcag
     5581 cttatacagc atcaaccaac ttcactacaa caggtggtgc tacttgcgtt gataattacg
     5641 aaccaaataa tagcagaggt acagcagctc tcgtatcaac aggcgtaact tatggcgaat
     5701 tgattgctac cagcactgat atcgactggt tcaaattcac taccacttca cctaatacta
     5761 aaattaaagt gaatatgact tcattacctg ctgactacga tattcgtctt tacgacaatg
     5821 ccggaactca gaaaggttct tcaatcaatg gtggaacaac agatgaacaa attatatgga
     5881 actcaacttc tgcaggcact cgctatatta aaattaacgg ctatgccgga gcattcaatg
     5941 cctcagattg ctatgatttc ttaatttctg ttgcttcaac taactggaaa actgatgaag
     6001 cttttgcaga tgttgcaggt aaagaatggg aaaatactat catgggtatc tatcctaacc
     6061 ctgtaacagg ttctgtagtt actgttgact acttcagcgt gttggaagat atggatgtta
     6121 ccgttcaagt attcgacgtg ctcggccgca tggtgagctc ttcagtagtt ggtatcgtta
     6181 atggtcctaa ctctatcgac ctcaatgttg ctgatcttca atcaggtatg tatttcgtaa
     6241 cgatcagctc tgcgaaattc atgcaatcac aaaacttcat tgtagaataa tacatttctg
     6301 caatcaacct ataaaagagg ctgtcttatt aaggcagcct cttttttttg gtgagtggtg
     6361 agtggtgagt gggcagtggg tcagtgggtg agtgggtgag tgaggcaatt tcacgactca
     6421 cgattcacga ctcacgattc acgactcacg atattagtat
//
LOCUS       DAOQHY010000171         6448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_6485_length_6448_cov_5.646176, whole genome shotgun sequence.
ACCESSION   DAOQHY010000171 DAOQHY010000000
VERSION     DAOQHY010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6448
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6485_length_6448_cov_5.646176"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..474
                     /locus_tag="PLL28_12070"
     CDS             121..474
                     /locus_tag="PLL28_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70104.1"
                     /translation="MHRILKDNGVAIVAVPSTFNLFSTHIATTIFKLMGKRKRFVDKP
                     YHVFEYTPRTISALFKKEFREVQVVNRIKKPSQLNMKTGGFEYKIKYLIHVINYPFTK
                     LFNRNGDRLLVVARK"
     gene            complement(762..2504)
                     /gene="ggt"
                     /locus_tag="PLL28_12075"
     CDS             complement(762..2504)
                     /gene="ggt"
                     /locus_tag="PLL28_12075"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNF70105.1"
                     /translation="MNCYLYTMRYSIFLVIWLWSCTSSTQPADKPVTDNPGVSLEGRT
                     HGVLGDSAMVVCAHPIAAQVGADILRKGGNAVDAAVAVQFALAVVYPNAGNIGGGGFM
                     VMRSQDGSTTALDFREKAPSASDRDMFINKQTGEVDRKKIETSHLASGVPGSVAGMWE
                     AHQKYGTLPWADLIQPAIDLAENGFPVTKNQAEDLRGLQRELKSLNPGKNYFLKNDWK
                     EGDLLIQQDLANTFIAIRDNGRDGFYSGEVAKKIVAEMSSHNGIISGDDLKNYQAVWR
                     TPVQGTYRGYTIISMPPPSSGGVALMQLLNMMEPFPLSDWGFHTTATMSIMVEAEKRV
                     YADRATWLGDPDFAKVPTTELTAKSYAAQRMLGVDTNTVTPSKNIKAGNIPGYESEET
                     THFSIVDQFGNAVAITTTLNDSYGSRIVVAGCGFILNNEMDDFSAKPGEPNLYGLIGG
                     EANAIAPNKRMLSSMTPTIVLKDNKLFMVIGSPGGSTIITSVFQNIVNVIDFNMTMQE
                     SVNASRFHHQWLPDEIKSETSMDEVVLQKLKAKGYTLSSREAIGRVDAILIHEGSLFE
                     GAPDPRGDDAAVGW"
     gene            2561..3814
                     /locus_tag="PLL28_12080"
     CDS             2561..3814
                     /locus_tag="PLL28_12080"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNF70106.1"
                     /translation="MPEAVNYIVYSIPVFFLLIGVEILIDKARKTGYYRLNDALSNIN
                     AGITEQVTGAFLKTFVVGLYAWTYDHMRIASIPDNIVMWIILFIGVDFFYYWFHRLAH
                     EINVLWGGHVVHHQSEEYNLSVALRQGAFQKFGSFLFYLPLALIGFNPVMFIVVSQFQ
                     TLYQFWIHTKVVNKLPAPFEFILNTPSHHRVHHGRNPKYIDKNHGGTLIIWDRMFGTF
                     QDEEEEVIYGITKPLKTWNPLRAQLDFWRDLWHDLARTPGIGDKIKLLLLPPGWYPAV
                     LGGPQTPPSINKQTEKKFDTHISMAMNTYVFIQFVITLGITSWFLFTYETMTLSSGLI
                     VMAFVLISITATGLLLEGNRWAVFVERLRLIGLPLVFFYLHYIGFFGIIPVRYYNPLL
                     AYVVVSLIIFTFILRRNSLHVKNIK"
     gene            3795..4502
                     /locus_tag="PLL28_12085"
     CDS             3795..4502
                     /locus_tag="PLL28_12085"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HNF70107.1"
                     /translation="MSKTSSNTGFLNIYVLIGASHIIALALHVDIVVMLTKFLLMPAL
                     MLHVYKHVGVSTVLRNRLLFVLFFSWMGDILLMFDENNSNFFIFGLAAFLIAQAAYIT
                     AFSIAAHKSNGSSLPPGLLLIPPIYAGILLSILIPHLGNMLIPVVIYSMVITAMLMQS
                     LRRWSKTDNYSFWFVAIGATFFIASDSMIAIDSFYSPINFAPLLIMITYIIAQYMLVT
                     GLLLHKDKVINQPQSDQ"
     gene            complement(4477..5499)
                     /locus_tag="PLL28_12090"
     CDS             complement(4477..5499)
                     /locus_tag="PLL28_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNF70108.1"
                     /translation="MMDEQRMNIRLQKIVVAIAIILLLVKFSAYLLTHSNAILTDALE
                     SIVNVVAGAFGLYSLILASKPRDINHPYGHGKIEFISASIEGSMITLAGVLIIGKSIF
                     NLLYPQTIQNVESGIILVAIAGTVNFAMGIFMERRGKKANSLILIAGGKHLQSDGWST
                     AGLLVGLGLLLIFKKPCIDSAVALLFGAYIAYEGYQILRKSVAGIMDEADTELLRSLI
                     DTFEKHREDDWIDIHNLRVIKYGANLHFDCHLTVPYYYSVKEGHDALERMESLIHVHH
                     ETSDEWFVHIDACVPPAMCKICTKRDCQHRQSPLESRIEWNLENVLRNQKHGLYGPTI
                     DPTEVD"
     gene            complement(5668..>6448)
                     /locus_tag="PLL28_12095"
     CDS             complement(5668..>6448)
                     /locus_tag="PLL28_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HNF70109.1"
                     /translation="SNNPNLQNIPIRTERGQKVRRAFIPRDKHHKILSADYSQIELRI
                     IAALSNDPAMIDAFKNGMDIHSATAARVFGVPINEVTREMRSKAKAVNFGIAYGQTAF
                     GLSQNLNISRGEAKEIIDNYNAQFPGVKQLMESNIEFARKHGYTQTILGRKRYIKDIN
                     STNNTVRSGAERVAVNSPIQGSAADLIKVAMLHIHEALNTKKMHSRMLLQVHDELVFD
                     AHVDELDELKALVVEKMVNAIKMNVPIEAEVGIGDNWLEAH"
BASE COUNT         1748 a         1329 c         1345 g         2026 t
ORIGIN      
        1 tgatgctttg tgtggcaatt ttgagaacat cgacctgtcg gcacatctac ataatgggat
       61 tgtgtgtacg gtggcgatgt attcgaacat tttattcatc ctgatcttgt ggtgaaaaat
      121 atgcatcgca ttttaaaaga taatggcgtt gcaatagtgg cagtgccatc aacctttaat
      181 ttattctcta cacatattgc tactacaatt tttaaactga tgggaaagcg caagcgattt
      241 gtggataagc cctaccatgt gtttgaatat acacctcgaa ccatcagtgc gctatttaaa
      301 aaagagtttc gcgaagtgca ggtggtgaac agaattaaga agccttcgca gttaaatatg
      361 aagaccggag gatttgaata caagatcaaa tacctcatac atgtcatcaa ttatccgttt
      421 acaaaattgt tcaaccgcaa tggcgacagg ttgctggtgg ttgcgaggaa gtgatcaatt
      481 cccattgttg cttatctgca tcgctgccca tagttgaaac ggatgcatct caggtaagga
      541 ggagccgtca gtgctgaaaa ccctgaactt agccggatac catcatccga acacgatggc
      601 agcggcgttt ttccgtaatc aggcaaaacc aaattattga aaatcgcaac tcaccgcaga
      661 tgcctaattg cgatatttgt tgtaagctgc cactcacttt gtcagatttg ttggactctt
      721 gtattgcaca gcctgagcat cgattttcat tattaaaatt ttcaccatcc aacggcagca
      781 tcatcaccac gtggatcggg agcgccttcg aataatgagc cttcatgtat taaaattgca
      841 tctacgcgtc cgatggcttc ccgagaggag agggtgtatc ctttggcttt taatttttgc
      901 aatacaacct cgtccatcga tgtttcagat ttgatttcgt caggcagcca ttgatgatga
      961 aatcttgagg cattcacact ttcctgcatg gtcatattga aatcaattac gttaacgata
     1021 ttctgaaaca cactggtaat aattgttgag cctccgggag atcctatcac cataaataat
     1081 ttattatcct tcaatacaat agttggcgtc atgctgctta gcatgcgttt gtttggggcg
     1141 atggcatttg cttctccgcc aatgagtccg tataaatttg gttctccggg tttagcactg
     1201 aagtcgtcca tctcattatt taaaataaat ccgcatccgg caacaacaat tctcgatcca
     1261 taactgtcat ttaaagttgt ggtgatggca acagcatttc cgaattgatc tacgatactg
     1321 aaatgagttg tttcctcact ttcatatccc gggatatttc ctgctttaat atttttcgat
     1381 ggtgtaaccg tattcgtatc aacaccaagc atgcgttgag cagcgtatga ttttgcagta
     1441 agttctgttg ttggcacttt agcaaaatcc ggatctccga gccatgtggc gcgatcggca
     1501 taaacgcgtt tttctgcttc caccatgata ctcattgttg cggtagtatg aaatccccaa
     1561 tcactcaaag gaaagggttc catcatattc agcaattgca ttaatgcaac accaccgcta
     1621 cttggaggag gcatggaaat gattgtataa ccgcgataag taccttgaac cggtgtgcgc
     1681 catacagcct gataattttt caaatcatcg ccggaaataa tgccattgtg tgatgacatt
     1741 tctgctacaa tttttttcgc tacttcgcca ctataaaatc catcgcgacc attatcgcga
     1801 atggcaatga atgtatttgc cagatcctgt tgaatcagta agtcgccttc tttccaatca
     1861 ttttttagga aataattttt tcccggattt agcgatttta attcgcgttg aagtccgcgt
     1921 aaatcctctg cctgattttt tgtaacagga aaaccgtttt ctgcgaggtc gatagcaggt
     1981 tggataagat cagcccatgg gagggttcca tatttttgat gtgcttccca catacctgcc
     2041 acacttcccg gtacgccgga tgccaggtgt gatgtttcta ttttttttct gtcaacttct
     2101 cctgtttgtt tattgataaa catatcgcga tcggatgccg agggtgcttt ttctctgaaa
     2161 tcgagagcag ttgtgctacc atcctgactg cgcatcacca taaatccgcc gccaccaata
     2221 ttgccggcat tgggatatac taccgcaagt gcaaattgaa cggctacagc ggcatccact
     2281 gcattgccac ctttgcgcaa gatatctgcg cctacctgag ctgcgattgg atgtgcgcaa
     2341 accaccatgg ctgaatcgcc taaaacgccg tgggtccttc cctccagtga aacgccgggg
     2401 ttgtcggtaa caggtttgtc ggcaggctgc gtagaggagg tgcagctcca caaccagata
     2461 actaagaaaa tggaatatcg catggtataa agatagcagt tcataagcag agcctccaag
     2521 gttacctgaa aactattaca tttgaacaat atcattttgt atgcccgaag cagtaaatta
     2581 tattgtttat tccattccgg tattcttttt attgattggt gtggagatac ttatcgataa
     2641 agcgaggaaa acaggttact atcgtttgaa tgatgctttg tcgaatatca atgccggcat
     2701 aacagagcag gtaaccggtg catttttgaa aacatttgta gttggattgt atgcatggac
     2761 ttatgatcat atgcgcatcg ctagtattcc ggataatatt gtgatgtgga tcatcttatt
     2821 tattggcgta gatttttttt actattggtt tcatcgcctt gcacatgaaa ttaatgtgtt
     2881 gtggggcgga catgtagtgc atcatcaaag tgaggaatac aatttaagtg ttgccttgcg
     2941 tcaaggagca tttcaaaaat tcgggtcatt tttattttat ctgccattgg cgcttatagg
     3001 tttcaatcct gtgatgttta ttgtggtgag tcagtttcaa acattatatc aattttggat
     3061 tcataccaag gtggtcaata aacttcctgc gccatttgaa tttattttaa atacgccatc
     3121 gcatcatcgt gtgcatcatg gcagaaatcc gaagtatatc gataaaaatc atggtggcac
     3181 tttgattatc tgggatagga tgtttggtac atttcaagat gaggaggaag aagtaattta
     3241 cggcattaca aaaccactca aaacatggaa tccgcttcgc gcgcaactcg atttctggag
     3301 agacctttgg catgatcttg cgcgcactcc cggtattgga gataaaatca aattgttgtt
     3361 gctgcctccc ggctggtacc ctgcggtatt aggtggaccg caaacaccac catcgataaa
     3421 taaacaaaca gaaaagaaat ttgatacgca tatttcaatg gcgatgaata cttatgtgtt
     3481 catacaattt gtgatcacat tgggcataac gtcatggttt ctatttactt atgaaacaat
     3541 gacattatca agtggcctaa ttgtcatggc ttttgtttta atcagcataa ccgcaaccgg
     3601 gttacttctg gaaggaaata gatgggctgt gtttgtggag cgacttcgat taataggatt
     3661 gcctctggtg tttttttatt tgcattatat cggcttcttt ggcatcattc ctgtcaggta
     3721 ttataatcca ttgttggcct atgtggttgt ttctttaatc atatttacat ttattttacg
     3781 acgaaattct ttgcatgtca aaaacatcaa gtaataccgg atttttgaat atatatgtat
     3841 taattggcgc atcgcatatt attgcactag ccttgcatgt agatattgtt gtcatgctta
     3901 caaagttctt attgatgccg gcattgatgt tgcatgtata taaacacgta ggggtatcaa
     3961 cagtgctgag aaacagattg ttgtttgtat tattcttttc ttggatgggt gatattttgt
     4021 tgatgtttga tgaaaataat tccaactttt tcatcttcgg actggctgca tttctaatcg
     4081 cacaagctgc atacatcact gcattttcca tcgctgcaca taaatctaat ggaagttcac
     4141 tgccgccggg attattattg attccaccaa tttatgcagg tatattgtta tctattttaa
     4201 tcccacacct tggcaatatg ttgattcccg tagtaattta ttcaatggtt attaccgcaa
     4261 tgctgatgca atcgttgcgc aggtggtcga agactgataa ttatagtttt tggtttgttg
     4321 caattggcgc tacgtttttt attgcctctg attcaatgat agccattgat tcattttatt
     4381 cacccataaa tttcgcacca ctgctgatca tgattacata tatcatagca caatatatgc
     4441 ttgtaacggg attgctgttg cataaagaca aggtgatcaa tcaacctcag tcggatcaat
     4501 agttggtccg tagagaccat gtttttgatt gcgaagtacg ttttccagat tccattcaat
     4561 gcgtgattcc aagggagatt ggcgatgctg acaatctcgc ttcgtgcaaa tcttgcacat
     4621 ggcaggtgga acacaggcgt cgatatgtac aaaccattca tcagaagttt cgtggtgcac
     4681 atgtatcagt gactccatgc gttccagtgc atcgtgccct tctttaactg aataataata
     4741 tggaacagta agatgacaat caaagtgcag atttgcacca tatttaatta cgcgaagatt
     4801 atgaatatct atccagtcgt cttcacgatg tttttcaaat gtatcgatga gagaacgcaa
     4861 taactcggta tccgcttcat ccatgatgcc ggcaacactt ttgcgcaata tttgatatcc
     4921 ttcgtaggca atatatgcac cgaataataa tgccacggca ctatctatac atggtttttt
     4981 gaaaatcagc aacaagccga gtccaaccaa taaaccggcc gtactccagc cgtcactttg
     5041 caaatgttta cctccggcga tgaggatgag tgagtttgct tttttacctc ggcgctccat
     5101 gaaaatcccc atggcaaaat ttacagttcc tgcaattgca accagaataa tacctgattc
     5161 aacattctga atggtttgcg gatacaataa attaaaaatg gactttccga taatcagcac
     5221 tcccgccaag gttatcatac taccttcaat gctggcggaa ataaattcaa tttttccgtg
     5281 accataagga tgattgatgt cgcggggttt tgaggcaagg atcaaactat ataatccaaa
     5341 agcacctgca acaacattta caatgctttc aagtgcgtcg gtaagaattg cgtttgaatg
     5401 tgtaaggaga taagcactga attttaccaa aagcaaaata atggctattg caaccacaat
     5461 tttctgcaac cgaatgttca tccgctgctc atccatcatg caaaggtatt gaaatattaa
     5521 ttcaagtagt gggtagtgag tagtgagact agctcaatac tgactgtaac ttcgttacag
     5581 taaatgcatt aaaactctgc gtctttgcgc ctctgcgtga aaaaaagact cacgattcac
     5641 gcttcacgac tcacgatact cacccactca atgcgcttcc agccaattat cgccaatgcc
     5701 cacttcggct tcaatgggga cattcatttt gatagcgttt accatttttt caactaccaa
     5761 tgctttcaat tcatcgagtt catcgacatg tgcgtcgaag accaattcat catgcacctg
     5821 gagcagcatg cgcgaatgca tttttttagt gttgagggct tcgtgaatat gtagcatggc
     5881 tactttaatc aggtcggcgg cagagccttg gataggagag tttacggcaa cacgttctgc
     5941 tccactgcgg actgtgttgt tggtagaatt aatatccttg atatagcgct ttcggcccaa
     6001 aatagtttgc gtgtaaccat gcttgcgggc aaattcaata ttgctttcca tcaattgctt
     6061 cacacccggg aactgcgcat tgtagttatc aatgatctct ttggcttctc cgcgagaaat
     6121 atttaagttt tgtgagagtc cgaatgctgt ttgtccgtat gcgataccaa aattcactgc
     6181 cttggcttta ctgcgcattt cacgtgtcac ttcgttgata ggaacaccaa atactcgcgc
     6241 ggcagttgca ctgtgaatgt ccatgccatt tttaaaagca tcgatcatgg caggatcatt
     6301 actgagtgct gcgatgatgc gcaactctat ttgagaataa tcggcgctga gtattttgtg
     6361 atgtttatcg cgtggaataa atgctctgcg cactttttgt ccgcgttcgg tgcgaatggg
     6421 aatattttgc aaattcggat tattggat
//
LOCUS       DAOQHY010000172         6443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_6497_length_6443_cov_5.561678, whole genome shotgun sequence.
ACCESSION   DAOQHY010000172 DAOQHY010000000
VERSION     DAOQHY010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6443
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6497_length_6443_cov_5.561678"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..591
                     /locus_tag="PLL28_12100"
     CDS             220..591
                     /locus_tag="PLL28_12100"
                     /inference="COORDINATES: protein motif:HMM:NF025607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 family protein"
                     /protein_id="HNF70110.1"
                     /translation="MNAEFFLWVNAVLYAGFAAWCAISPTSTAAFSGFSFMNVNGRSE
                     YFALYVGLQAAWSVMFAICAINNDLQYAGVFYAVFLYAGVIFGRWVSIFQKGVAGNNV
                     YIIAILEIILGAWAVLLLSNF"
     regulatory      690..788
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1204..2517
                     /locus_tag="PLL28_12105"
     CDS             1204..2517
                     /locus_tag="PLL28_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HNF70111.1"
                     /translation="MSNYQYETLQLHAGQQADPVTGSRAVPIYQTTSYVFNDSAHAAD
                     LFGLKAFGNIYTRIMNPTTDVFEKRIAALEGGVAALAVASGQAAQTIAITTIAQAGDN
                     IVSTSFLYGGTYNQFKVAFKRLGIDVRFAEGDDIEAFEALIDSRTKGIYLETIGNPGF
                     NIPDLEKFAALAKRHDIPLIVDNTFGAAGYIAQPIKYGANIVIASATKWIGGHGTSIG
                     GVIVDAGNFNWGNGKFPLFTEGSEGYHGLKFWDTFGAGSPFGNIAFIIRARVEGLRDL
                     GPAISPFNAFLLLQGLETLSLRVQRHCDNAQQLAEWLEQHPLVEYVNYPGLTSSPYHA
                     LADQYLTNGYGGVLSFKVHGGTTNANKFVDALKLVSHLANVGDAKSLIIHPASTTHEQ
                     LSAEEQLDAGVKPGLLRLSVGIEHINDIIADLEQAFALIETVVAV"
     gene            2536..3567
                     /gene="metX"
                     /locus_tag="PLL28_12110"
     CDS             2536..3567
                     /gene="metX"
                     /locus_tag="PLL28_12110"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNF70112.1"
                     /translation="MQHNYCLPEGFQLENGQFIPELRLRYHTYGQLNAQRDNVIWVFH
                     ALTANAEPDIWWNGLFGEGNILDPSCYLIVCVNMPGSCYGSTGPEDTDRYGRKYAETF
                     PVFTIRDMVLAYQKLRDALQIKKILFAIGGSMGGMQALEWAVVEPDFIENLVLLACNH
                     QHSPWGIAYNAAQRMAIEADTSFLHGDGKKGLEAARAIAMLSYRNYRTFCLTQQHDET
                     VSQHKAESYLRYQGKKLSKRFSAQSYNILTKSMDSHNIARNRGDVHAALQRIKAKTLV
                     IGIDSDILFPVIEQAFLAKHIPGAALEVISSNYGHDGFLIESGKIKRAINSYFPSLNS
                     QRKLCKTIL"
     gene            3549..4730
                     /locus_tag="PLL28_12115"
     CDS             3549..4730
                     /locus_tag="PLL28_12115"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNF70113.1"
                     /translation="MQNNTLNIGLFGYGCVGKGFASLATNYPELNLRIKKICIRDAEK
                     PRDLDAEYFTTDANSLLLDDEINVIVELISDADAAFLIVKQALCNGKAVVSANKKMLA
                     EHLEELVSLQQQFGVPLLYEGSVCGSIPVIRNLENYYDKEPLLGIEGIINGSTNYILT
                     KLHANDIEYKTALAEAQALGFAEADPTLDVEGYDPVNKLCILLLHAFGIFIKPESIFH
                     KGITGINAVDIAFAKKHHSRIKLLAKATQTEAGIQAYVLPHLVNGNSNLTAIDFETNS
                     VAIHAAWSDLHQLTGKGAGSIPTGSAVLSDVLAIRKNYCYPYKKSVYNDNKPTLVDGI
                     PLRIYLRAEASSLVDTTIFEEIEETFKSEDNTFYIGKTKLENLSKASWVKDSGVSLIA
                     L"
     gene            4930..5502
                     /locus_tag="PLL28_12120"
     CDS             4930..5502
                     /locus_tag="PLL28_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNF70114.1"
                     /translation="MFSVQFSEIVTISLTLFAIIDVLGTVPAIINMKSRGIVLQPGKT
                     GLFAGALMVTFLFLGDTILRLLGLDTQSFALAGSVVIFLLGLEMILGMHIFKSNTESG
                     SAGSLVPIAFPLLVGAGTLTVLISMRSIYSTVNILVAVVINSIVIYVVLRSTDWIEQR
                     VGKAGLHAITKFFGVIALALAIKIFLANMM"
     gene            5565..6173
                     /locus_tag="PLL28_12125"
     CDS             5565..6173
                     /locus_tag="PLL28_12125"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNF70115.1"
                     /translation="MKTALITIVFICTGLLIFACANNNGQSNMHPSAPEVRNATPNLP
                     LSSLMASAGLQAGDIWIYIDKSAFMLEIKHDTTTIKTYPVVLGGNPVGDKRMEGDQKT
                     PEGEFALRSIYPHKKWSKFLWVNYPTDESWEKFNAAKASGEIPKNASIGGEIGIHGVP
                     EGYDSLIDEMQNWTLGCISLKNAHIDEIYDAVKVGTRVVIKG"
BASE COUNT         1907 a         1276 c         1395 g         1865 t
ORIGIN      
        1 attttaattc cgaagcgcaa tcagctcccc tctctatcga cgataggaga tggagagggg
       61 ttgggggtga ggtgggcggg cgggtgaggc ggtttcgcgc aaaggcgcta agtggcaaag
      121 gcgcaaaact gacccactca cccactgacc cattcaccca ctcacccact gacccactca
      181 ccattcacga ttctacctta actttactaa aaacatatta tgaacgcaga attcttcctt
      241 tgggtaaacg cagtgcttta cgccggcttt gctgcctggt gtgctattag tccaacgagc
      301 accgcggcat tttcgggatt cagttttatg aatgtaaatg gacgttcgga atattttgca
      361 ctttatgtgg gattgcaagc tgcatggtcg gtgatgtttg ctatttgtgc tattaataat
      421 gatctgcaat atgccggagt attttatgcc gtattcttat atgccggtgt catcttcggc
      481 agatgggttt ctatatttca aaaaggtgtg gccggtaata atgtgtacat catcgccata
      541 ttagagatca ttcttggtgc atgggctgtt ttgctgctca gcaatttttg aaaaacaatg
      601 tggttcaagt aaaacaatta aaatattatc agataaatat ttggatgtct catttctaaa
      661 tatatatctt tgcatcagtt cttaagaatt acttatccag aaaggccgag ggactgaccc
      721 gaagacgcct tagcaaccct caaatgtttc tcgcatttgt gaaggtgcta atttcagaaa
      781 gataggttgg agagtcaaaa atattgcaag ctcaccaata catatcggcg agcttttttt
      841 atgcccgtca gtctcctgaa acctttatgt ggtaagcaat tcacaagatt ggaacacaat
      901 ttgctattaa cacaaaaggt aatgttcatc atgggtaaaa acattaaaac ctacacatta
      961 ttgtcgctgc aaagcagggg aattaattac tcaatttatc attcattttc cttcaggtat
     1021 atgtgttgcc atatggcaac tatgtaacta ttgcttccct ttccgggtgc gaatgatccg
     1081 atacgttcgg tagatcatgc gtgatatatt catgcaccat taatcggaga aaaatctccg
     1141 caagccactg ctatgcccaa cattttcaca attcaatttt aaaatatcat taaatcaaat
     1201 atcatgagta actatcaata tgaaacgttg caattacacg ccggacaaca agcagatcct
     1261 gtaacaggaa gtcgcgccgt gcctatttat caaactacat cctatgtgtt caacgacagc
     1321 gcacatgcag cagatctgtt cggattaaaa gcgttcggca atatctacac aaggattatg
     1381 aatccaacaa cagatgtctt tgaaaaacgt atcgctgcat tagaaggtgg tgttgctgca
     1441 ctggccgttg catcgggaca ggcagcccaa accatcgcca ttaccaccat tgcacaggcg
     1501 ggagataata tcgtttctac atctttttta tacggcggca catacaatca atttaaagtt
     1561 gcattcaagc gattgggtat tgatgttcgt tttgccgaag gtgatgacat tgaggcattt
     1621 gaggcactca tcgattcgag aacaaaaggt atttacctgg aaactatagg caatcccggt
     1681 tttaacattc ctgatctcga aaaatttgcc gcacttgcca aacgccatga tattccactt
     1741 atcgttgata atacattcgg tgctgcaggc tacatcgcgc agccgataaa atatggcgcc
     1801 aatattgtta tcgcgtcagc aacaaaatgg attggtggac acggaacaag tatcggcggt
     1861 gtaattgttg atgccggaaa tttcaattgg ggaaatggaa agttcccact cttcaccgaa
     1921 ggatctgaag gatatcacgg acttaaattc tgggatactt tcggtgccgg ttctcctttc
     1981 ggaaatatcg catttatcat tagagcacgc gttgaaggat tgcgcgatct tggtcctgcc
     2041 ataagtccgt ttaatgcatt tttactttta caaggtcttg agacattatc actgcgcgtt
     2101 cagcgccatt gcgacaatgc acaacagctt gccgaatggt tagagcaaca tcctttggtg
     2161 gaatacgtaa attatcccgg attaacatct tcaccctatc atgcgttagc tgatcaatat
     2221 ctgaccaatg gctatggcgg agtcttgtct tttaaagtac acggcggtac aacaaatgca
     2281 aacaaatttg tcgatgcctt aaaattggtg agtcacctgg caaatgttgg tgatgctaaa
     2341 tcgctgatta ttcatccggc ctcaactaca catgaacaac taagtgcaga agaacaactt
     2401 gatgcaggag taaaaccggg attactacgt ttatcagttg gcattgaaca tatcaatgat
     2461 atcatcgccg atttggaaca agcatttgct ttgattgaaa ctgtagttgc tgtttaattt
     2521 caaaaataat tcatcatgca acataattat tgccttcccg aagggtttca attagaaaac
     2581 gggcaattca ttcccgaatt gcgattgcgt tatcacactt atggtcaatt aaatgcacag
     2641 cgcgataatg ttatttgggt atttcacgca cttactgcaa atgccgaacc tgatatctgg
     2701 tggaatggtt tatttggtga aggcaatatt cttgacccaa gctgctattt gattgtttgc
     2761 gtaaatatgc cgggttcttg ctatggtagc acaggtcctg aagatacaga tcgatatgga
     2821 aggaaatatg ctgaaacctt tccggtattt accatacgcg atatggtgct ggcctatcaa
     2881 aaattgcgag atgcacttca aataaaaaaa atcttgtttg ctattggtgg ttccatgggt
     2941 ggcatgcaag ccttggaatg ggctgtagtg gagcctgatt ttattgaaaa tctggtgttg
     3001 cttgcatgta atcaccaaca ttcaccttgg ggaattgcat acaacgcagc gcaacggatg
     3061 gcaatagagg cagatacttc ttttcttcat ggcgatggca agaaaggtct ggaagcagcg
     3121 cgtgcaatag ctatgttatc gtatcgcaat tatcgcacct tctgtttgac acagcagcat
     3181 gatgaaactg tatcacaaca caaagcagaa tcgtacctac gatatcaggg gaagaagctt
     3241 tccaaaagat ttagtgccca aagctataat atactgacaa aaagcatgga cagccataat
     3301 atcgcccgca atcgaggaga tgtacatgcg gcacttcagc gtattaaagc aaagaccttg
     3361 gtgattggta ttgattctga tatactattc ccggtgattg aacaagcctt tctggctaaa
     3421 catattcccg gggctgcatt ggaggttatc agcagtaatt atggtcacga cgggtttctg
     3481 atcgaatcag gaaaaatcaa aagagccata aattcatatt ttccatcatt aaactcacaa
     3541 agaaaattat gcaaaacaat actttaaata taggattatt cggatatggt tgtgtaggaa
     3601 agggatttgc ttcactggca acaaattatc ctgaattgaa tcttcgtata aaaaaaatat
     3661 gtatcagaga tgcagaaaag ccaagagatt tggatgcaga atattttacg acagatgcaa
     3721 actctttact tctcgatgat gaaatcaatg tcatagtgga actcatcagc gatgccgatg
     3781 cagctttcct aattgtaaag caagcattat gcaatggaaa agctgtagtc agtgcaaata
     3841 aaaaaatgct ggcggagcat ctggaagaac ttgtttcatt acaacaacaa ttcggtgtgc
     3901 cgctgttata tgaaggttct gtttgcggaa gtattcctgt cattcgcaat ctggagaatt
     3961 attatgataa agaaccactg cttggcatag aaggtattat aaatggctcc acaaattaca
     4021 tccttacaaa attacatgcc aatgatatcg aatataaaac cgcattggcg gaggctcagg
     4081 cattaggatt tgcagaagcc gaccctactt tagatgtaga aggatacgac cctgtaaata
     4141 aactttgtat tctactttta catgctttcg gaatttttat aaaaccggaa tcaatttttc
     4201 acaaaggcat cacaggaatt aatgcggttg atatcgcatt tgcgaaaaaa catcatagcc
     4261 gcatcaaact cctggccaaa gcaacacaaa cagaagccgg catacaagct tatgtgcttc
     4321 cccacctggt aaacggcaac tccaatctca ccgcaattga tttcgaaaca aacagcgttg
     4381 ccattcatgc tgcctggtca gatttgcatc agcttacagg caaaggagcc ggcagtattc
     4441 cgacaggatc ggcagtattg agtgatgtat tagccatccg gaaaaactat tgctatccat
     4501 ataaaaaatc tgtgtataac gacaataagc ctacacttgt cgatggtata ccactgcgca
     4561 tttatttgcg ggcagaagct tcgtcgctag ttgacaccac tatttttgag gaaattgaag
     4621 agacgtttaa aagtgaagat aacacgtttt acattggaaa aacaaaactt gaaaaccttt
     4681 caaaagcctc ttgggtaaag gattccgggg ttagcctgat tgcactttga caaaaaaaag
     4741 aaaggaagtg aactttttaa ggtcggtaat tgtctgtatc tttgtaactg caatgaaatt
     4801 tactttgaac atatataaca acagcattgg catccatgag tatccatgga catgcaagtg
     4861 ccatgcttgc taaaagcata ctcccctgtt caccgttgtt attttttggc attttcaaag
     4921 ttcactttta tgttttctgt tcaattttca gagatcgtaa cgatcagctt aaccttattc
     4981 gcaattattg atgtattggg aactgttcct gccattatca atatgaaaag tcgcggcatt
     5041 gtactgcaac ccggaaaaac aggattgttt gctggcgcat tgatggtcac atttctgttt
     5101 ttaggtgata ccattttacg attgttgggt ttagacacac aatcatttgc ccttgcaggt
     5161 tctgtagtaa tttttctgct cggactagag atgatactcg gcatgcatat ttttaaaagc
     5221 aataccgaat caggcagtgc cggtagtctg gtgcctattg cttttccatt gttggttggc
     5281 gcgggcactt taacagtgct catcagcatg cgcagtatat attcaacagt gaatattctg
     5341 gttgcggtag ttattaatag tattgtcatt tatgtagtgc tgcgctccac cgattggata
     5401 gaacaacgcg taggtaaggc aggtttacat gccatcacaa aatttttcgg tgtgattgca
     5461 ttggcattgg cgattaagat atttcttgcg aatatgatgt aaaacctatt atagattaca
     5521 aagaatcgca aatacgaata ttgatacgtc atcgttaata ttccatgaaa accgcactga
     5581 tcacaattgt ctttatttgc acgggattac tgatatttgc ctgtgccaat aataatggtc
     5641 aatctaatat gcatccttct gcaccagaag taagaaacgc cactccgaat ttaccattgt
     5701 catcactgat ggcaagtgcc ggtttacagg caggagatat atggatatat atcgataaat
     5761 cggcattcat gcttgagata aaacatgata ccaccaccat taaaacatat cctgttgtat
     5821 taggcggaaa tcctgttggc gataaacgca tggagggcga tcaaaaaacg cctgaaggcg
     5881 aatttgcgtt acgttcgata tatccgcata agaaatggag caaatttcta tgggtgaatt
     5941 atcctacaga tgagagttgg gaaaaattca acgcagcaaa agcatcaggt gaaattccaa
     6001 aaaacgcaag cattggagga gaaattggta ttcatggcgt ccccgaaggc tacgactcgc
     6061 taatcgatga aatgcaaaat tggacactcg gttgtatctc cttaaagaac gctcatattg
     6121 atgaaattta tgatgcggtg aaggttggta ctcgggtagt tattaagggg tgattgggtg
     6181 agtgggtgag tgggtgagtg gtgagtggtg agtggtgagt ggtgagtggt gagtggtgaa
     6241 tggtgaatgt aattcggaac gttttgtgaa gagtgattcc tccgcaaact ccgctaactc
     6301 cgcgattgac cgtgtgcgat tggttgacct gtaaattgcg agcttgcctc gctaccggcg
     6361 gtgaatttat agagccaagt atctagactt tgcgccttag cctccttgct gtctttgcgc
     6421 gaaaccaacc cgcctcacct ccg
//
LOCUS       DAOQHY010000173         6356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_6639_length_6356_cov_5.149659, whole genome shotgun sequence.
ACCESSION   DAOQHY010000173 DAOQHY010000000
VERSION     DAOQHY010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6356
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6639_length_6356_cov_5.149659"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="PLL28_12130"
     CDS             complement(<1..1032)
                     /locus_tag="PLL28_12130"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNF70116.1"
                     /translation="MKKFKPFLKVAAWIIGGIVLYLVGVILYGTITEYKPEPETSEVV
                     EIKGKATASEKMDSVLNLMTWNIGYGGLGKEMDFFYDGGKAVRAPREIWDKDFKGICE
                     LMTANDSIDFFLIQEIDRDSRRSYEVDMYREVNHILKGYSASFGLNYDVNFVPVPYTH
                     PMGKVYGGVATYSKYQPAEALRYQYPGHFPWPTRIFFLDRCFLEQRYPLANGKELVLL
                     NTHNSAYDESGEIKDGEMQYLRKFVDAEYAKGNYVIVGGDWNQCPPNFKNDKFKPKSG
                     YDGFMPPSMSFDYASEGWIWPYDPDVPTNRHVETPLDDNTFRTLIDFFLISPNVELLK
                     IKTINNGFDF"
     gene            1106..1933
                     /locus_tag="PLL28_12135"
     CDS             1106..1933
                     /locus_tag="PLL28_12135"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HNF70117.1"
                     /translation="MNYQTYHWKQAAGQEIFAQSWLPDTTPKAIYCIIHGQSDHSSRF
                     THMAAYMVEKGFAVYAADLIGHGKSGGKRGHMLHFEEYLETVDALIELALKASPGLPV
                     FLYGQSMGGNVVINHALRGTDKVKAYIASSPWIRLAFEPPAWKVGLGKMMKSIYPALS
                     QPTGLNADFLSHDKQVVSDYVNDPLVHGKITASAFFETLQAGLQIPDKASHLKTPMLI
                     LHGSEDKITSSIASKSFAEMRPDLITHKLFDGCYHELHNESIKEQVFITIVQFIEKQ"
     gene            1938..2363
                     /locus_tag="PLL28_12140"
     CDS             1938..2363
                     /locus_tag="PLL28_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70118.1"
                     /translation="MDNKFLNTVRILFIGIFLAPLMFLGVILFTQSGQEKPLTVANDP
                     FFFIVVIMSVGAIIASTMLYKKRLPAIQEMTDTAAKLNAWKSLLIIKLAIIEGVTLFA
                     VVVLQIQLANIYLFIALALSAYQLTNIPTEEKIKTDCNL"
     gene            2376..2624
                     /locus_tag="PLL28_12145"
     CDS             2376..2624
                     /locus_tag="PLL28_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70119.1"
                     /translation="MKNLYIITFALLTAACFFSCDDKTIACGEILIQVSEAAQDWNSS
                     TANCEAYIDAMHSYLDNDCATPTVVDEYEGILDTLSCP"
     gene            2696..4150
                     /gene="glpK"
                     /locus_tag="PLL28_12150"
     CDS             2696..4150
                     /gene="glpK"
                     /locus_tag="PLL28_12150"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNF70120.1"
                     /translation="MQFILAIDQGTTSSRSILFDGEFRELAKSQQEFKQYFPQPGYVE
                     HDAEEIWTSQRETILNVLQRRDLNAVSAIGISNQRETVVVWDKHTGVPIHNAIVWQDR
                     RTAEICEQLKQQGHEQYIRENTGLVIDAYFSGTKIKWILDHVSGARDKANKGELLCGT
                     IDSWLIWKLTEGRVHATDVTNASRTMLFNIRSMQWDAKLLSFLDIPASMLPSVKMSHD
                     DFGYTILHGHQIPIRGVAGDQQAALFGQQCVQPGMVKNTYGTGCFMLMNIGEQFALSK
                     SGLLTTIAWGLDGKITYALEGSVFNAGAVIQWLRDSLQIISSSAESEDLARSIQGNDG
                     VYFVPAFTGLGAPHWDMYARGTITGLTRGTGKAHIARAALEAIAYQTRDVLDAMQRDT
                     GIPINSLRVDGGATTNNWLMQFQSDILQTTVFRPAYLESTALGAAMLAAYHQAILSQS
                     NKQNETIYQPLISLTSADDLYFQWNEAVNKCRSI"
     gene            complement(4154..5083)
                     /locus_tag="PLL28_12155"
     CDS             complement(4154..5083)
                     /locus_tag="PLL28_12155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70121.1"
                     /translation="MKTLLILLLAGIQVQVFAQFEEFWPINSEGWITEYNANGPPQAN
                     YRFYVEEDTLIGDYTYKVLMRDRINPYTASTTTKLFAYFREEDGVITYYDETTGGEWS
                     YFDQNATIGDTIKTIIHPDQQMDGCDEYRTYVVDSLSDTTISGTLLKVFFLKAAPFSC
                     YDEYAFFWESGKFIESIGFLAYPTPYLNAGAFDGDFPGMLKCYENDQLGLVQFITEPC
                     DFLETGIVQASLPEFNVFPNPANAVVQIDCGLKEVDRYSLMNALGQPMYSCEVQNNIR
                     LITVDVSTIPEGIYFLQMHFTDGSIGKSAFVRN"
     gene            5199..>6356
                     /gene="lpdA"
                     /locus_tag="PLL28_12160"
     CDS             5199..>6356
                     /gene="lpdA"
                     /locus_tag="PLL28_12160"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNF70122.1"
                     /translation="MNYDVIILGSGPGGYVTAIRASQLGLKTAIVERAELGGICLNWG
                     CIPTKALLKSAQVFDYMKHAADYGITVSDAKADFGAMVKRSRDVAGGMSKGVQFLMKK
                     NKIEVIEGNGVVKAGKSVEVTKADGTKSTITAKHIIIATGARSKDLPTMKQDGKKIIG
                     YREAMTLPLQPKKMVIVGAGAIGVEFAYFYHSIGTEVTIVEFMDRLVPVEDEEVSKEL
                     ARQFKKNGINIMTSSAVESVDTSGAGCKVQVKTAKGVETLECDIVLSAAGVVANIENI
                     GLEQVGIKTEKGKIVVDKYYRTNIEGYYAIGDVTPGPALAHVASAEGITCVEAIAGHH
                     PEAIDYGNIPGCTYCFPEIASVGMTEAAAKAAGYELKIGKFPFTASGKAKAG"
BASE COUNT         1904 a         1268 c         1374 g         1810 t
ORIGIN      
        1 gaaatcaaat ccattgttga tggttttaat tttaagcagt tcaacattcg gtgagatcaa
       61 aaagaaatca atcaaggttc tgaaagtgtt atcatcgagc ggtgtttcaa catgtcgatt
      121 agttggcaca tccggatcat aaggccagat ccaaccttcc gaggcataat cgaatgacat
      181 acttggcggc ataaatccat cataacccga ttttggtttg aatttgtcat ttttgaaatt
      241 cggcggacac tgattccaat cgcctccaac aattacataa ttaccttttg catattcagc
      301 atccacaaat tttctgagat attgcatttc accatcttta atttctccgg attcatcgta
      361 tgccgagtta tgtgtattca ataatactaa ttctttccca ttggctaatg gatagcgttg
      421 ttccaaaaaa catctgtcga gaaagaaaat tcttgtcggc catggaaaat gtccgggata
      481 ttgatagcgt aatgcttcag caggttgata cttactgtag gtagcaactc cgccatacac
      541 ttttcccata ggatgtgtat atggaacagg tacaaagttg acatcgtaat ttaatccgaa
      601 tgatgcacta tatcctttca ggatatgatt gacctcgcga tacatatcta cctcataact
      661 tcttcgagaa tcgcgatcta tctcctgaat caaaaagaaa tcgatactat cgtttgccgt
      721 cattaattcg caaattcctt taaaatcttt atcccaaatt tcacgaggtg cacgcactgc
      781 cttgccgcca tcatagaaaa aatccatttc ttttcccagt ccgccatatc cgatattcca
      841 tgtcatcaga tttaatactg aatccatctt ctcagaagcg gttgctttac ctttaatttc
      901 cacaacttct gaagtttccg gttcgggttt atattcagtg atggtgccat acaggataac
      961 gccaaccaag tataaaacaa tgcctccgat gatccaggca gcgactttca aaaagggttt
     1021 aaactttttc atggtgtgaa gatacgcctc cgtgcaatat gttggtcggc acttaacttc
     1081 gatttaattc gtattttcga ccgttatgaa ttatcagacc tatcattgga agcaggctgc
     1141 aggccaggaa atttttgccc aaagctggct tcccgacact actccaaagg ccatttattg
     1201 cataatccat gggcaaagtg atcacagttc gaggttcacg catatggctg cttatatggt
     1261 ggaaaaaggt tttgccgtgt atgctgcaga cctgataggg catggtaaat caggcggcaa
     1321 acgcggacat atgctgcatt tcgaggagta tctagaaaca gttgacgctt tgatagaatt
     1381 agcattaaag gcaagtcctg ggcttccggt atttctgtat ggccaaagta tgggaggaaa
     1441 tgttgttatc aatcatgcac ttcgaggcac tgacaaggtg aaagcatata tcgcatcttc
     1501 gccttggatc cgactggcat ttgaaccgcc ggcgtggaag gtgggattgg gaaaaatgat
     1561 gaaatcaatt tatccggcct tatcgcaacc aacaggatta aatgccgatt tcctttcgca
     1621 cgataagcaa gtagtttctg actatgtcaa cgatcccttg gtgcatggaa aaataacagc
     1681 cagtgcattt tttgaaactt tgcaagcagg tttacaaatt cctgataagg catcacattt
     1741 aaaaacacca atgctcattt tgcatggttc ggaggataaa attacgagtt ctattgcctc
     1801 caaatcattt gctgaaatgc gtccggatct gattacacat aagctttttg atggttgtta
     1861 tcatgaatta cataacgaat ctattaagga gcaagttttt ataacgattg ttcaatttat
     1921 agaaaagcaa taattacatg gataataaat ttctaaatac ggtaagaatt ttgtttatcg
     1981 gaatattttt ggcgccatta atgtttttag gtgtcatttt atttacgcaa tcgggacaag
     2041 aaaaaccgct tactgtagca aatgatccat ttttctttat tgtggtcatc atgtcggttg
     2101 gcgccattat agcttctacc atgctataca agaaaagact acctgcaata caggaaatga
     2161 ctgacaccgc agccaagtta aatgcatgga agtcgctgct gattattaaa cttgccatta
     2221 tcgaaggtgt tacactattt gctgttgttg tgcttcagat acaattggca aatatttact
     2281 tatttatagc attagcattg agcgcatatc aacttacaaa tattccaacg gaagaaaaaa
     2341 taaaaacaga ctgtaacctt taataaaact tcaacatgaa aaatctatac atcatcacct
     2401 tcgcattatt aacggcagcg tgtttctttt cctgcgatga caaaacaata gcatgtggag
     2461 aaatccttat acaagtgagc gaagcagcac aggattggaa ttcatcaact gccaattgcg
     2521 aagcctacat cgatgctatg catagctacc ttgacaatga ttgtgcaacg ccaacggtgg
     2581 ttgatgagta tgaggggata ttggatacgt tgagttgtcc ttgacattat tcacactcgc
     2641 tcgcccaatt ttcacgcaga ggcgcaaaga cgcagaggaa taaagccgca aagaaatgca
     2701 attcatatta gcaattgatc agggaactac gagctcacga agtattttgt ttgatggaga
     2761 atttcgtgaa cttgctaagt cgcaacagga attcaaacaa tattttcctc agccgggata
     2821 tgttgaacat gatgcagagg aaatatggac gagtcagcgg gaaacaattt taaatgtatt
     2881 acagcgaaga gatttgaatg cagtttcagc gattggcatt tcaaatcaac gcgagacagt
     2941 agtagtttgg gataaacaca ccggagttcc tattcacaat gcaatagtat ggcaggatcg
     3001 ccgtacggca gagatatgcg agcaattaaa gcaacaggga cacgaacaat atatccgtga
     3061 aaatacagga ttggttattg atgcatattt ctccggaact aaaatcaagt ggatcctcga
     3121 tcatgtatca ggggcgagag acaaggcgaa taaaggcgag ttgttatgcg ggactatcga
     3181 tagttggctt atttggaaac tcaccgaagg ccgtgtgcat gcaactgatg ttaccaatgc
     3241 atcgcggacg atgttgttca atattcgcag catgcaatgg gatgcaaaat tgttatcttt
     3301 tttggatatc ccagcttcga tgttgccgtc agtaaaaatg tcgcatgatg attttgggta
     3361 tacgattttg cacggacatc aaattcctat tcgcggcgta gcaggagatc agcaggctgc
     3421 attatttggt cagcaatgtg tgcaaccggg tatggtgaag aatacttatg gcacgggttg
     3481 ttttatgttg atgaatattg gcgaacaatt tgctttaagt aaaagcggat tgctcaccac
     3541 catagcatgg ggacttgatg gcaaaattac ctatgcttta gaaggaagtg tttttaatgc
     3601 cggcgctgtt attcagtggt tgcgagatag cttgcaaatt atttcgagta gtgctgaatc
     3661 agaagatctt gccaggtcga ttcagggaaa tgatggagtc tactttgttc ctgcatttac
     3721 aggattaggt gcgccacatt gggacatgta tgcacgagga actatcacag gattgacgag
     3781 aggtaccggt aaagcacata ttgcacgtgc tgcattggag gctatcgcat atcaaacgcg
     3841 cgatgtatta gatgccatgc agcgcgacac gggaattccg ataaattcat tacgcgttga
     3901 tggcggtgca accacaaata attggttgat gcaatttcag agtgatattc tgcaaacaac
     3961 agtgtttcgt ccagcctatt tagaatccac agcactgggt gcggcaatgc tggctgctta
     4021 tcatcaggcc atattatcac agtccaataa acaaaacgaa accatctacc aacctctaat
     4081 tagtttgacc tccgctgatg atctttattt tcagtggaat gaggctgtca ataaatgtag
     4141 gtcgatctaa ttattaattt ctgacaaatg ctgattttcc aatactgcca tcagtaaaat
     4201 gcatttgaag gaaatatatg ccttcgggaa tagttgatac atcaacagta ataagtcgaa
     4261 tgttattttg cacttcgcag gaatacatag gttgtcccag ggcgttcatt aaagaatatc
     4321 tatccacctc ctttaatcca caatcaatct gcaccacagc gttagccgga ttcgggaaca
     4381 cgttaaactc cggaagagat gcttgcacta ttcctgtctc cagaaaatcg caaggttctg
     4441 taatgaattg caccaaaccc aactggtcat tttcataaca tttcagcata cccggaaaat
     4501 cgccatcaaa tgcgcccgca ttaagatatg gcgtagggta agctaaaaat cctatcgact
     4561 caataaattt tcccgattcc caaaaaaagg catattcatc ataacaacta aacggtgcag
     4621 cttttaggaa aaacaccttc agcaatgtac ctgatatggt agtatctgaa agactgtcga
     4681 caacatacgt tctgtattca tcgcaaccat ccatttgttg atccgggtgg atgatcgttt
     4741 taatagtatc gccgattgtt gcattttgat cgaaataact ccattcacca cctgttgttt
     4801 catcgtaata ggtaattaca ccatcctcct ctctgaaata tgcaaacaac ttcgtagtgg
     4861 tagatgcagt ataaggatta atcctatcgc gcatcagcac cttatatgta tagtcgccta
     4921 tgagtgtatc ttcttcaaca taaaaccgat aatttgcttg tggcggacca ttggcattat
     4981 attcagtgat ccagccttcg ctgttgatgg gccaaaattc ttcgaattgg gcaaatacct
     5041 gaacctggat acctgccaat agaaggatta aaagtgtttt cataatttaa atatacaaca
     5101 aatatggttc atcttaaaca gcccttagag ggaggcatgg ctttcgcatt gtcctttccc
     5161 accacgtacc gtatctttgc acccgcaaat cgacttttat gaactacgat gtaataatat
     5221 tgggcagtgg ccccggagga tatgtaaccg ccatcagagc atcacaatta ggcctgaaaa
     5281 cagcaattgt ggagcgtgcc gagcttggag gtatctgttt gaactggggt tgtataccta
     5341 ccaaagccct cctgaaaagc gcccaggtat tcgattacat gaagcatgcg gccgactatg
     5401 gcatcactgt ttccgacgct aaagccgatt ttggggcgat ggtaaaacgc agtcgcgacg
     5461 tagcaggcgg catgagcaaa ggcgttcagt tcctgatgaa gaagaataaa attgaggtga
     5521 ttgaaggaaa cggcgttgtt aaagcaggta aatctgttga agttacaaag gctgatggca
     5581 ctaaatctac catcaccgct aagcatatta tcattgcaac aggtgcgcgc agtaaggatc
     5641 tgccaactat gaaacaggat ggcaaaaaga tcattggtta tcgcgaagca atgacacttc
     5701 cgcttcaacc taagaaaatg gtgatagttg gtgcaggtgc cattggtgtt gaatttgctt
     5761 atttctatca ttccattggc actgaagtga ccatcgtgga atttatggat cgcctggtgc
     5821 ctgttgagga tgaagaagtg tctaaagaat tggcacgtca gtttaaaaag aacggcatca
     5881 atatcatgac aagctctgct gttgaatctg tggatacttc aggggcaggt tgcaaagtac
     5941 aagtgaaaac tgcgaaaggt gttgagacgc tcgaatgcga tatcgtattg agtgctgcag
     6001 gcgttgttgc caacattgaa aatattggtc ttgaacaagt aggtattaaa actgaaaaag
     6061 gcaagatcgt ggttgataaa tattatcgca ccaatatcga gggatattat gctattggtg
     6121 atgtcactcc cggaccggca ttagcacacg ttgccagcgc agaaggtatt acatgtgttg
     6181 aagcgattgc aggacatcat ccggaagcaa ttgattacgg taatattccg ggatgtactt
     6241 attgcttccc tgaaattgcg agtgtgggta tgactgaagc tgctgcaaaa gctgcgggat
     6301 acgaattgaa gatcggaaaa ttcccattca cagcttccgg taaagcaaaa gcaggt
//
LOCUS       DAOQHY010000174         6298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_6728_length_6298_cov_5.545411, whole genome shotgun sequence.
ACCESSION   DAOQHY010000174 DAOQHY010000000
VERSION     DAOQHY010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6728_length_6298_cov_5.545411"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PLL28_12165"
     CDS             complement(<1..392)
                     /locus_tag="PLL28_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70123.1"
                     /translation="MKKSYLLFMLMVFVVAANAQVTPSCPTDVKGAEITDKTLVEFTP
                     TLLTDETWGTPTLLTDRRVFFVHGLGGEGDEAGTTAISWMQASLWSEQQYMINSARPD
                     YNNVSLEAAAMELKGDLESFADANSDAI"
     gene            complement(404..1516)
                     /locus_tag="PLL28_12170"
     CDS             complement(404..1516)
                     /locus_tag="PLL28_12170"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70124.1"
                     /translation="MRFMLTLRNGVAGACAVPTNMHVPRLSAKWLMLVCVIALSASHT
                     KAQTTLLYTINYVADRTTMYVYPDSLQSYLIHEQIALLPVYQRDSVIRMVYTNKDVKN
                     ITYYKADSRFEDWMTRPAKSIVDKKSVKLYNSSGMLMLTQTHSADYKALYSDLKTQMS
                     TLGTDLIPDYVQLTNTLKSNLLANGFVMTNLGGGTYRFVKDSVEVRYNNSRKMNEMRL
                     YHADGSLKYALLRDFAVNSFGQTTPVLTQERRPDTRFPGGCVEEVVTTKYPTYYMVYG
                     GTGKIEEDDMAEDELYISPNPATTETTISYPPEQTDNATAMVEVFDLTGKMVIQKYVN
                     ASEGSVVINTSSLEAGVYAVIYTCALLKLESQFVKQ"
     gene            complement(1780..2640)
                     /locus_tag="PLL28_12175"
     CDS             complement(1780..2640)
                     /locus_tag="PLL28_12175"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HNF70125.1"
                     /translation="MQAQLSQQNYIVYNTRTKQIENLDSIVAEMQHVDVLFFGEEHND
                     SVGHYLEATLFEMMHKSYGAKTALSMEMFDRDVQPVMDEYLAGLIKEKQFVKDARVWS
                     NYRDYKPMVEYAKTNNLHVVCANAAGRYSSLAVRKGMQGLMALPEASKDHIAPLPFDT
                     ASGPYFDKLVDVMSGHDTTAGAAAVTAPGMDMVMGQSTWDATMAYSIAQYLKAHKGDK
                     VFQVNGRFHSDEHYAVVTQLKNYRPKATSLVISSGSDENFPNINWDDHKADGDYIIIT
                     DPKVPKTFEE"
     gene            complement(2768..3520)
                     /locus_tag="PLL28_12180"
     CDS             complement(2768..3520)
                     /locus_tag="PLL28_12180"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNF70126.1"
                     /translation="MSHTLFIGKRHIRLASADSTNNWLKGYLEVHPTAPEGLAITASD
                     QTNGRGQSGKRWESAPGLNLAVSYLLRPTFLAPAGMFTLNKAIALAVRDTVAEFLPET
                     RIKWPNDIFSGNHKVAGILIESSVNSRVQWVVAGIGINVNQMKFGPEAPRAGSIAAIS
                     GHKIHIDDLLQKLHIHLEHHYLRLRAGHDADMHQAYLDHLMAYQQSFRFIHDNQSFQG
                     VLTGVDPEGRMIVDMNGKITSFAMGEITWEFD"
     gene            3556..3930
                     /gene="rsfS"
                     /locus_tag="PLL28_12185"
     CDS             3556..3930
                     /gene="rsfS"
                     /locus_tag="PLL28_12185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNF70127.1"
                     /translation="MRKSKKQALPLQYSLTELVIDSILDKKGEEVVHLDLTALPDAVS
                     DHFIICHADSTTQVRAIGNHILENVFEKSGDRPFSKEGLDNAEWIIIDYMDVVVHVFY
                     KEKRYFYQLEDLWHDAVMKKVS"
     gene            3949..5883
                     /gene="ftsH"
                     /locus_tag="PLL28_12190"
     CDS             3949..5883
                     /gene="ftsH"
                     /locus_tag="PLL28_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNF70128.1"
                     /translation="MSTEQNNQNKTKPNGNRKFNFYWIYAIIAVILISFQFFPFQGSP
                     VVLKDISTFKDDMLLKGDVNRIVVVNKEYAEIFIKEDSLKKKIYTDDKVPEKGAQYKW
                     IIGDTETFERQLTEWQEGKTSQPVTIMYETRHNILVSLLSWILPFVIILVIWILVMRR
                     VTGGSGMGGGGQIFNIGKSKPQMYDKDTKVNVTFNDVAGLDEAKEEVMEVVDFLKNPK
                     KYTSLGGKIPKGVLLVGPPGTGKTLLAKAMAGEAQVPFFSISGSDFVEMFVGVGASRV
                     RDLFRQAREKAPCVIFIDEIDAIGRARGKNMIQSNDERENTLNQLLVEMDGFGTDKGV
                     IILAATNRPDVLDPALLRPGRFDRQISIDKPDLAGREQIFNVHLKPIKVSPEVDAHKL
                     AVQTPGFAGADIANVCNEAALIAARRGKKFVELQDFNDAIDRVIGGLEKKNKIISQEE
                     KKIVAYHEAGHAICGWYLEYADPLVKVSIVPRGMAALGYAQYLPKEQYLYTKEQLTDM
                     MCMTLGGRAAEDIIFGRISTGAQNDLERITKMSYAMVSIYGMNERIGNISFNDPNMDQ
                     RFSKPYSEQTAQAIDEEVKKMIDSAYQRTKKLLNEKRDQLEIIAKELLEKEIIFKADM
                     ERLIGHRPYDEKHPDLTHVG"
     gene            5970..>6298
                     /locus_tag="PLL28_12195"
     CDS             5970..>6298
                     /locus_tag="PLL28_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70129.1"
                     /translation="MAKILSSKEKMLSSVRGALLHQTRQPYPNLESNDNIYNVPDEPL
                     EIIFATEFTAVSGQFVFCETEKECVDTLKELVAHKKWEHIVCPEPLLHELFDRQQFIK
                     YDADAPVT"
BASE COUNT         1799 a         1277 c         1436 g         1786 t
ORIGIN      
        1 aaaatagcat ctgagtttgc atcagcaaaa gactcgaggt cgccctttaa ttccattgca
       61 gctgcttcga gtgaaacatt attataatcc ggacgggcag aattaatcat atattgttgt
      121 tcgctccaca atgaagcctg catccagcta attgcggtgg ttcctgcttc atcaccttca
      181 ccacccagtc catgcacaaa aaacaccctt ctgtctgtaa gcaaggttgg tgttccccat
      241 gtttcatctg taagtaatgt tggggtgaat tcaactaaag ttttatcggt gatttctgcc
      301 cccttaacat cagtagggca cgatggagtt acttgtgcgt tagctgcaac tacaaacacc
      361 atcagcataa aaagtaaata gctctttttc ataataattg taattattgt tttacgaatt
      421 gagattccag tttcagcaat gcgcaggtat atatcacggc gtacacacct gcttcgagtg
      481 aagatgtatt tattactact gaaccctccg aggcattcac atatttttga ataaccattt
      541 ttccggtgag gtcgaatact tctaccattg cggtagcatt atctgtttgc tccggcgggt
      601 aagaaatagt agtttctgtt gtagccggat ttggtgaaat atataattca tcttccgcca
      661 tatcatcctc ttcaattttt cctgttcctc cgtaaaccat ataataagta gggtattttg
      721 ttgttacaac ttcttctaca catcctcccg gaaatcttgt atccgggcga cgttcctgag
      781 taagcaccgg tgttgtctgg ccgaatgaat tcacggcaaa atcgcgcagc aacgcatatt
      841 tcaaggaacc atcggcatga tacaatcgca tttcattcat ttttcgagaa ttattatacc
      901 gcacttccac cgaatctttt acaaagcgat acgtgccacc accaagatta gtcatcacaa
      961 aaccattggc gagcaaattc gacttcaagg tatttgtcaa ttgcacataa tcgggaataa
     1021 ggtccgtgcc gagtgtcgac atttgtgttt tcagatctga atacaatgct ttgtaatcgg
     1081 cactatgcgt ttgagtgagc attagcattc cgctgctgtt gtaaagtttc acgcttttct
     1141 tgtcgacaat actttttgcc ggtcgcgtca tccaatcttc aaagcgacta tccgctttgt
     1201 agtaagtaat gtttttgaca tccttgttgg tgtataccat gcgaattacg ctatcgcgtt
     1261 ggtaaacagg cagcagtgca atttgctcat gaattaaata cgactgcagc gaatcagggt
     1321 acacgtacat tgtagtgcgg tcagccacat aattaatagt gtaaagtaag gtagtttgtg
     1381 ctttggtatg cgatgcggag agcgcaatta cacataccaa catcaaccat tttgcggaca
     1441 acctcggcac atgcatgttg gttggaacag cgcatgcacc ggcaactcca tttcggagcg
     1501 tgagcataaa tctcatacga aaattattta agggttaaac aattaattat cgctacaaca
     1561 ataaattgtt tttcatttcc ctcaaaatat ttcgaagact ttgtaagcgc ttaggttaac
     1621 attgatttta tcaacgaaaa ttgtaatcaa acaaaaagtt gatataaatg agacgtaagc
     1681 ggtttaatga aatttgaatt gtggatattt taattccaat aattaggatg atgcagcctt
     1741 tgaattgcca tatcatttat gatatcacta aataaatcat cactcctcaa acgtcttcgg
     1801 caccttaggg tcggtgatga tgatataatc gccatcagcc ttatggtcgt cccaattaat
     1861 attcgggaag ttttcatcgc tgccgctgct gatgaccagt gatgtggctt tagggcggta
     1921 atttttcaat tgtgtaacaa cggcataatg ctcatcgctg tggaatcttc cgtttacctg
     1981 aaatacttta tcacccttat gcgctttcag gtattgtgca atactgtaag ccatagttgc
     2041 atcccaggtt gattgtccca tgaccatatc cattccgggt gcggtaactg cagctgcacc
     2101 ggcagtggta tcgtgaccgc tcattacatc caccaactta tcaaagtacg gaccgcttgc
     2161 tgtgtcgaaa ggaaggggtg caatatgatc tttgcttgct tcgggtaagg ccatgagtcc
     2221 ctgcatccct tttcgcacgg cgaggcttga atatctgccg gcggcgttag cgcacactac
     2281 atgcaaatta tttgttttgg catattccac catgggttta tagtcgcgat aattgctcca
     2341 cacacgcgca tctttcacga attgtttttc ttttatcaat ccggcaagat attcatccat
     2401 gaccggctgc acgtcgcggt cgaacatttc catcgacaag gcggtttttg ctccgtaaga
     2461 tttgtgcatc atctcaaata atgttgcttc gagataatgg cctacagagt cgttgtgttc
     2521 ttcgccaaaa aacaagacat caacatgctg catttcggca actatggaat ccaggttttc
     2581 aatttgttta gtcctggtat tatagacaat ataattttgt tgagagagtt gagcctgcat
     2641 tgtaatagtt atgcaggata ccaaaatgag tatcaaatgt cgcatgatgt aaaattacga
     2701 aacgaagatt agaataagcg agcaacaaat cattgaagaa aggcaattat tatcacctta
     2761 gcatctttca atcaaattcc caggtgattt cgcccatggc aaatgacgtg atttttccat
     2821 tcatatcaac tatcattcgt ccttccggat ccacacctgt caatacaccc tgaaaacttt
     2881 ggttatcatg tataaatcga aaagattgct gataggccat cagatgatca agataagcct
     2941 gatgcatatc agcatcgtgt ccggcgcgca aacgcagata atgatgttcg aggtggatat
     3001 gcaatttttg caaaagatcg tcgatatgga ttttatgccc gctgatagcg gcaattgacc
     3061 ctgccctggg agcttcgggg ccaaatttca tctgatttac attaatgcct atacctgcca
     3121 ccacccattg caccctgcta ttgacagaag attctatgag tatgcctgcc actttgtggt
     3181 tacctgaaaa gatgtcgtta ggccatttaa tgcgtgtttc gggtaaaaat tctgccacgg
     3241 tatcacgaac cgcaagggca atggctttgt taagagtgaa cataccggca ggggcgagga
     3301 aggtaggtcg cagcaggtag ctgaccgcca gattcaatcc cggtgctgat tcccatcgtt
     3361 ttcctgactg tccgcggcca ttagtttggt cggaagcggt tatggccaaa ccctcgggag
     3421 ctgtaggatg cacctcaaga tagcctttca gccaattgtt ggtgctgtcg gcagatgcca
     3481 gtcggatatg acgctttcct ataaagaggg tatgggacaa aagaattcgt aattttgcaa
     3541 cctttaacaa agtaagtgag aaaatcgaaa aagcaggcat tgcctctgca atattcctta
     3601 acagagctcg tcatagacag tatccttgat aaaaaagggg aagaagtggt tcatttggat
     3661 ctgactgctc taccggacgc tgtcagtgac cattttatca tttgtcatgc cgattccacc
     3721 acgcaggtgc gggcaattgg aaaccatatt ctcgaaaatg tgtttgagaa gtcaggtgac
     3781 cgcccgttca gcaaagaggg actcgacaac gccgaatgga tcattatcga ctatatggat
     3841 gttgtagtgc atgtttttta taaagagaag cgttacttct atcaactcga ggacctgtgg
     3901 catgatgccg tgatgaaaaa agtgagttaa taccgtatat actattcaat gagtaccgaa
     3961 cagaataatc aaaacaagac aaaaccgaat ggcaatcgca aattcaattt ttattggata
     4021 tacgcgatca ttgctgtgat tcttatcagt ttccagtttt tcccttttca aggctcccct
     4081 gtcgtgttga aagatatcag cactttcaaa gacgacatgt tattgaaggg tgacgtaaac
     4141 cgcattgtag tggtgaataa ggaatatgcc gaaatcttta tcaaagaaga cagtcttaag
     4201 aaaaaaatat acaccgacga taaggttcct gaaaaaggtg cacagtataa atggatcatc
     4261 ggtgatactg aaacattcga gcgtcaacta actgaatggc aggaaggcaa aaccagtcag
     4321 cctgttacta tcatgtatga aacacgccat aatattttgg tgagtttatt gagttggata
     4381 ttaccgttcg tgatcatact tgtgatttgg attcttgtga tgcgcagggt aaccggcggc
     4441 tcgggaatgg gcggtggagg tcagatattt aatatcggca agtcgaaacc gcagatgtat
     4501 gacaaggaca cgaaagtgaa tgtcaccttt aatgatgtgg caggattgga tgaggcgaag
     4561 gaagaggtga tggaggttgt agacttcctg aaaaacccga aaaaatatac ttcacttggg
     4621 ggtaaaattc ctaaaggtgt gttactggta ggtcctccgg gaacaggtaa aacattgctt
     4681 gcaaaggcga tggctggaga agcgcaggta ccattcttca gtatttcagg ttccgatttc
     4741 gtagagatgt ttgtgggtgt aggtgcgagc cgtgtgcgcg accttttccg tcaggcacgt
     4801 gagaaagcac cttgtgtgat ctttattgat gaaatcgatg ccattggtcg tgcgcgtggt
     4861 aaaaacatga tacaaagcaa cgacgaacgt gaaaatacat tgaaccagtt attggtggaa
     4921 atggatggat tcggcactga taagggtgta atcattctgg cagctaccaa ccgtccggat
     4981 gtattagacc ctgcattatt gcgtccgggt cgatttgacc gtcagatatc tattgataaa
     5041 ccggatcttg caggtcgtga gcagatattc aatgtgcatt tgaaaccgat caaagtttcg
     5101 cctgaagtag atgcgcataa attggctgtg cagacaccgg gatttgcagg tgctgatatc
     5161 gcgaacgtat gtaacgaagc tgcattgatc gctgcacgtc gcggaaagaa atttgttgaa
     5221 ttacaagatt tcaatgatgc cattgatcgc gtgattggcg ggttggagaa aaagaataaa
     5281 atcatttcac aggaagagaa aaagatcgtt gcttatcacg aagcaggtca tgccatttgt
     5341 ggctggtatc tggaatatgc cgatccactg gtgaaagtat ctattgttcc gcgtggtatg
     5401 gctgcattgg gatatgcgca gtatctgcca aaagaacaat atctgtatac aaaagaacag
     5461 ctcaccgata tgatgtgtat gacattaggt ggtcgtgctg ctgaggatat tattttcggt
     5521 cgtatcagca ccggtgccca aaatgacctg gagcgcatca cgaagatgtc gtatgccatg
     5581 gtgagcattt acggtatgaa tgagcgcatt ggaaatatca gtttcaacga tccgaatatg
     5641 gatcagcgat tcagcaaacc atactctgaa caaactgcgc aggctattga tgaagaggtg
     5701 aagaaaatga ttgattccgc atatcagcgc acaaagaaat tgctgaacga aaagcgcgat
     5761 cagctcgaaa tcattgctaa agaattgctt gaaaaagaga tcatttttaa agctgatatg
     5821 gaaaggttga ttgggcatcg tccgtacgat gaaaaacatc ctgacttgac gcacgtgggg
     5881 tagaaggaga gaggagagag gaaaaaggag agaggagaga ggattggggt gtggggagat
     5941 ttgattaatt ttacatcaag ttatctttaa tggccaagat ccttagttcc aaagaaaaaa
     6001 tgctaagcag tgtgcgtgga gcactcttgc atcaaactcg ccagccatat ccgaatctgg
     6061 aaagcaacga caatatctac aatgttcctg acgaaccgtt ggagattatt tttgctaccg
     6121 agtttacagc tgtttcgggt caatttgttt tttgtgagac cgaaaaggaa tgtgtagaca
     6181 ccctcaagga gctcgttgcc cacaagaaat gggaacatat tgtttgcccg gaacccttgc
     6241 tgcatgagct tttcgaccgt cagcaattca taaaatacga tgccgatgct ccggtaac
//
LOCUS       DAOQHY010000175         6280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_6756_length_6280_cov_4.692369, whole genome shotgun sequence.
ACCESSION   DAOQHY010000175 DAOQHY010000000
VERSION     DAOQHY010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6280
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6756_length_6280_cov_4.692369"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..957
                     /locus_tag="PLL28_12200"
     CDS             262..957
                     /locus_tag="PLL28_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70130.1"
                     /translation="MKNLVTLTLALITFNIAFAQYTAVGIDVSTIAPVNNYCAKIQLE
                     RCFSFNQSIGLQLNYFADTTTSSGLVAEPYYRFYTGSLEDQHRFYLQLSTYAGQVMHT
                     EVYDNPEENVLFESWTNNQDAFTQLFFSLLDMDEHQYITKRFKQNILGAGISCGFTHR
                     FGNSNWSMYAQAGVKGTSYSTDVPETVIIAETTYTHVEGTIFNSTPQGYTTIDKSRVC
                     NVFTGGLGITYSF"
     gene            complement(1026..1883)
                     /locus_tag="PLL28_12205"
     CDS             complement(1026..1883)
                     /locus_tag="PLL28_12205"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNF70131.1"
                     /translation="MKRICVLILFSLWVWSVTAANPAAGDSVGVKVVDGKSLVVYKLS
                     AGETAYAVSRKYGINFKDLSVANSGLNMDQLKAGQEILVPTGRAANTSTANSNIAKVE
                     TAPREEVVQLKEAVIEAPVVAVPKTPVAETVPEKAEEVATTTEAAAEQTPALSSGDLE
                     ETPATVNNDKSKSFAQIYGGYLDGDHVSKSEKGVATWIESAGIEPSGDRFYALHNSAP
                     IGSVVKVRNMMNNRTIYAKVIGTLSESEVQEKVMIKLSAGAAERLNVLDNRFVSEITY
                     FIPDEQASK"
     gene            2348..3052
                     /gene="kdsB"
                     /locus_tag="PLL28_12210"
     CDS             2348..3052
                     /gene="kdsB"
                     /locus_tag="PLL28_12210"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNF70132.1"
                     /translation="MQTFAIIPARYASTRFPGKPLVEIQGKSMIRRVYEQAQSANIFS
                     RVVVATDDERIFNHVQAFGGEVVMTSPHHLSGTDRCGEVLASGKYACDVVFNIQGDEP
                     FIQPEQLQLLHKVFENPQAEIATLVKSISDEKDIANPNVVKVVFSKQGQAMYFSRSAI
                     PFARNIGATYYRHIGLYAFRSNILSQLVTLAPTDLERSESLEQLRWLENGFHINVVET
                     QMDTIGIDTPDDLLKI"
     gene            complement(3055..4455)
                     /locus_tag="PLL28_12215"
     CDS             complement(3055..4455)
                     /locus_tag="PLL28_12215"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNF70133.1"
                     /translation="MHTNEQILQRNIDITLPHDDFYEDLRAREFGRLDTNKQIYLDYT
                     GGNLYALTQLEKHVALLKNNIFGNPHSMNPASAFSTQLVEEARTKVLQFFNAEDYYCI
                     FTPNATGALKIVGESYPFEENGRLLLLADNHNSVNGIREFCITKDGKFDYATIHYEDL
                     MIDADSLDEKLSKPCNCDHKLFAFPAQSNVSGVRHDLSWVTRAQELGWDVLLDAAAYV
                     PTSKLDLRTIQPDFVSISFYKIFGYPTGIGCLLVKKDKFELLHKRWFAGGTVTMVSIL
                     SPHHFLASNHERFEDGTVNYLDIPAISTGLDFIETIGMHRIHARVDGLIQYLTESIAT
                     LQHDNGRPLIRIFGPDHHTHAGGTLIMNVLDINGDQYPFESVERNASDHGISIRSGCF
                     CNPGIDETNYCLTEEELARFYSSRDKGNYADMKLYLGKMRGAIRVSVGYATVQRDIDA
                     FIAFLKTYLNVSVDVQ"
     gene            complement(4616..5449)
                     /locus_tag="PLL28_12220"
     CDS             complement(4616..5449)
                     /locus_tag="PLL28_12220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF70134.1"
                     /translation="MSLSIRSYNTVDTFLQDAAVFLRPFSLENNRILGICTALGTAGK
                     NEMPYAIVVLEQMKVRGVFMVFDGVVLIGFRSDDEAIKLAATHLQHSNVQITSAMGTT
                     HEAQQFAAAFDKPIAKERNFLLYGAVDIRKVKVAKGEARFAESGDQAILMQWFELFFH
                     EANLYPPKTKDQIASIKSQQINSRSAMIWMVEGQPVSLAAIIRTTPDIAIIGHVFTPE
                     HARGKGYSASLIYKMCDVFKKRGHGRFGLYVDDENHTAKGVYHRLGFTEVCKCMQIIF
                     S"
     gene            complement(5472..>6280)
                     /locus_tag="PLL28_12225"
     CDS             complement(5472..>6280)
                     /locus_tag="PLL28_12225"
                     /inference="COORDINATES: protein motif:HMM:NF027464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HNF70135.1"
                     /translation="NAQQSPLPIKVVSSVTPIIDTSGSLLSTRIHPPTGYVRLSVDSN
                     SFGYYLRHLPLKPHEASVHYYDGSIKMNPNIYIAVVDQPIGNKDLHQCADAVMHLRAD
                     YLWQTGQYDRIHFDLTNGFRMEYAQWMAGNRLVVSGNQTSWKKTAEASNTASDLWAYL
                     EKVFTFAGTLSLSRELVSVERDDMQPGDVFIKGGSPGHAEIVVDMAMDTITGKKIFLL
                     AQSFMPAQETQILCNNEEPEISPWYNLDFGTSLHTPEYWFNDTQLMRFQD"
BASE COUNT         1739 a         1439 c         1261 g         1841 t
ORIGIN      
        1 gctaactccg caaactccgc gattgaacaa gggtgaatcg tgagtggtga gtttgctttt
       61 ccctctgcgt attctgcgtg aaaacctttg cgcctttgtg aaaaaaaaag caatcctatg
      121 gggtttattt tcaataaaat aaaccccata gccgcttata agcgtttcat ttgcgaaaat
      181 ccgtaattct tgtccgatca cctgcaaaaa ctgcaactcg ccggttgggg tccggtctac
      241 ttttgcatga aatcaaatgc tatgaaaaac cttgtaaccc ttaccctggc cctcatcacc
      301 ttcaacattg ccttcgctca atacaccgct gtcggcattg acgtaagcac catcgctccc
      361 gtaaataact attgcgctaa aatacagctt gagcgctgtt tctctttcaa ccaatccatc
      421 ggcttacaac tcaattactt cgccgataca acaaccagtt caggtcttgt tgccgaacct
      481 tattaccgtt tttataccgg atcgcttgag gatcaacatc gcttttattt acaactaagc
      541 acttatgccg gtcaggttat gcataccgaa gtgtatgaca atcctgaaga aaatgtttta
      601 ttcgaatcat ggacaaacaa ccaggatgca ttcacgcaat tatttttcag tctgctcgat
      661 atggatgaac atcagtacat cacaaaacga ttcaaacaaa atattttagg cgccggtatt
      721 tcatgcggat tcacacaccg ttttggcaac agtaactgga gcatgtatgc acaagccggt
      781 gttaaaggca cttcttattc caccgacgta cctgaaacag ttatcattgc tgaaactacc
      841 tacacgcatg ttgaaggaac catttttaat tctacaccac agggatatac caccatcgat
      901 aaaagtcgcg tgtgtaatgt atttacaggc ggactgggta ttacatattc attctgatag
      961 tatcaataaa caaaaaaaaa gcggtctgca taacaaaccg ctttcacaca cttattaacg
     1021 aacgatcatt tggatgcctg ctcgtcgggg ataaaatacg ttatctccga tacgaaacgg
     1081 ttgtccaata catttaatct ctctgcagca cctgcactca gtttgatcat cactttttcc
     1141 tgcacttcac tttcactcag ggtgcctatt actttagcgt aaatagttct gttgttcatc
     1201 atattgcgca ctttcaccac cgatcctatc ggagctgaat tgtgcaatgc ataaaacctg
     1261 tcgcccgatg gttcaatacc ggcgctctct atccaggtag caacgccttt ctccgattta
     1321 gatacatgat ctccatccaa atacccacca tatatctgcg caaatgattt cgatttatcg
     1381 ttgtttaccg ttgcaggtgt ttcctccaga tcgccgctgg aaagtgcagg tgtttgttct
     1441 gctgcagctt ctgtcgttgt agcaacttct tctgcttttt caggcactgt ttccgcaaca
     1501 ggtgttttcg gaaccgcaac taccggagct tcaataaccg cttctttgag ttgaaccact
     1561 tcctcgcgag gagctgtttc aaccttcgct atattcgaat tcgcagttga tgtgtttgct
     1621 gctcttccgg ttggaaccaa aatttcctgt cctgctttca gctgatccat attcaatccg
     1681 ctgttggcta ccgacaggtc tttgaagttg ataccatact ttctgcttac cgcgtaagct
     1741 gtttcaccgg cactcaattt gtaaactacc agcgatttac cgtcaaccac cttcacacct
     1801 accgaatcgc cggctgcagg gttggcagca gtcacgctcc atacccaaag gctgaagagg
     1861 atgaggacac atattctttt catattcttg tcttgtaaca gtgcttccat aatataactc
     1921 agctcccagg gttattattg tttatgcaca atttgttcac atgcaaaagt aattgcgtca
     1981 acctgcgcta acctattgaa agtggacatg ttatgcaccg cagatttcga cagtgtactg
     2041 ttatgcacac agccctatta gcgctaaaat gtggatttag cagcctttca cccttccgga
     2101 atatcgctca acatccttct aagcgcttag aagggagcat aacggtatga atgcgcaaaa
     2161 cccaataccg gcaagccttt caggttgttt gttgcctgct tccggctttc attaccttgt
     2221 atttccaact gcattacatc gggtgcttgc ttcaaatgaa tagcgaatac gcttattttt
     2281 gcctgtagag acctgttctt gtggaaggca cttaatcgcc tgcataagga tatgtccata
     2341 cccaattatg caaaccttcg ccattattcc cgccagatac gcctctacaa gatttcccgg
     2401 aaagccgttg gtggagattc agggtaagtc gatgatacga agggtttacg agcaggcaca
     2461 atcggcaaat attttttccc gggtagttgt agcaacggat gacgagcgta tttttaatca
     2521 tgtgcaagca tttggtggcg aagtggttat gacttccccc caccacctct caggcaccga
     2581 ccgttgtggc gaagtgcttg cctcaggaaa atatgcatgc gatgttgttt tcaacattca
     2641 aggcgatgag ccttttattc aaccggaaca attgcaatta ttgcacaagg tatttgaaaa
     2701 cccacaggca gaaattgcca ctttagtgaa gtcgatttcc gatgaaaaag atatcgcaaa
     2761 tccgaatgtg gtgaaagtag ttttttctaa gcagggacag gcgatgtatt tttcccgcag
     2821 cgcaattcct ttcgccagaa atattggtgc aacgtattac cgtcacattg gcttatatgc
     2881 cttcagaagt aatattctca gtcaactggt tacattagct ccaacagatt tggaacgcag
     2941 cgagtcgctg gaacaattgc gatggctgga aaatggattt cacatcaacg ttgttgaaac
     3001 gcagatggat accattggta ttgacacacc ggacgacttg ttaaaaattt aatgttactg
     3061 cacatcaact gaaacattca gataggtttt tagaaatgca ataaacgcat caatatcgcg
     3121 ctgcacagtt gcatatccta cagaaacacg aatagcgccg cgcatttttc cgagatacaa
     3181 tttcatatcc gcataatttc ctttgtcgcg actagaataa aaacgggcga gttcttcttc
     3241 tgttaagcaa taatttgttt catcgattcc gggattacaa aagcatccgg atcggataga
     3301 aatgccatga tcacttgcat ttcgttccac tgattcaaat ggatattgat cgccattaat
     3361 atccagcaca ttcattatca aagttccccc tgcatgtgta tgatgatcag gtccgaaaat
     3421 tcggatgaga ggcctgccat tatcatgttg caaggtagcg attgattcag tgagatattg
     3481 tatcaagccg tcaacgcgcg catgaatgcg atgcatgccg atagtttcaa tgaaatcaag
     3541 acctgtgctg attgcaggaa tatccagata atttacggta ccatcttcaa atcgctcgtg
     3601 attggaagcc aaaaaatggt gtggtgacag gatggatacc atggtgacag taccacctgc
     3661 aaaccaacgt ttgtgcaata attcgaattt atcttttttc accagcagac atccgattcc
     3721 ggtgggatat ccaaatattt tataaaagct aatactcaca aaatcaggtt gaatggtgcg
     3781 caaatcaagc ttgctggttg gcacatacgc tgctgcgtcc aataacacat cccatcccaa
     3841 ttcctgcgcg cgcgttaccc atgacaggtc gtgacgcaca ccgctaacat tcgactgtgc
     3901 aggaaatgca aataatttat ggtcgcaatt acatggcttt gataattttt catcgagact
     3961 atctgcatct atcatcaaat cctcgtagtg aatagtagca taatcaaatt ttccgtcttt
     4021 ggtgatgcag aattcgcgga taccatttac cgaattatga ttatcggcaa gcaacaacaa
     4081 tctgccattt tcttcgaaag gataactctc acctactatt tttaatgccc ctgtggcgtt
     4141 aggtgtgaaa atgcaataat aatcttcggc attaaaaaat tgcaatactt tagtccgtgc
     4201 ttcttcaacc aattgtgttg aaaaagcaga agcaggattc atggaatgcg gattgccaaa
     4261 aatattgttc ttcaataatg caacatgttt ttcaagttgg gtaagtgcat atagatttcc
     4321 gccggtgtaa tcgaggtaaa tttgtttgtt ggtgtctagt cgcccgaatt cccgcgcacg
     4381 caagtcctca taaaaatcgt cgtgcggtag tgtgatgtcg atattgcgtt gtaaaatttg
     4441 ctcgttagta tgcatatcat tcgtttggca gagcaaaggt aatatgcata cataatggta
     4501 aagatgactt atatcacaat agccgtcgtt tccctgcgat tatcaataaa ttttacctga
     4561 attaaaggtt gaatccaaat acatgcaatc aagcgcatgc attgtcatgg taccatcacg
     4621 aaaagataat ctgcatgcat ttgcaaactt cggtgaatcc caggcgatga taaaccccct
     4681 ttgccgtatg gttttcatca tctacataca atccgaacct gccatgtccg cgttttttga
     4741 atacatcgca catcttgtaa atcagcgatg ccgaatatcc tttgccgcgt gcatgttcgg
     4801 gtgtaaaaac gtgaccgatg atggcgatgt caggcgttgt tcggataata gctgccagac
     4861 ttacaggctg cccttccacc atccatatca ttgcagatct gctattgatt tgttgtgatt
     4921 taatgcttgc tatctggtcc tttgtttttg gaggatagag attcgcttca tgaaaaaata
     4981 attcaaacca ctgcattaaa attgcctgat caccactttc cgcaaatcgc gcttcacctt
     5041 ttgcaacttt aactttgcga atatcgacag ctccatacag taaaaaattg cgttccttgg
     5101 cgataggttt atcgaaggct gcagcgaact gttgcgcttc atgcgttgtg cccatagctg
     5161 atgtgatttg aacattgcta tgctgcaaat gtgtggcggc aagtttaatg gcttcgtcgt
     5221 ctgatctgaa acctatcagc actaccccat caaaaaccat aaacacgcct cgcaccttca
     5281 tttgttcgag cacaacaata gcataaggca tctcattttt tcctgcagta cctaatgccg
     5341 tgcagattcc taaaatgcgg ttattttcca atgaaaatgg tcgcaagaaa actgctgcat
     5401 cctgcaaaaa ggtatcaacc gtattataac ttcgaatgga tagtgacata gcaataatgc
     5461 aaaattgcac attaatcctg gaagcgcatc aattgcgtat cattgaacca atattcaggt
     5521 gtatgcaagg atgtgccgaa gtcgaggtta taccagggac tgatttccgg ttcctcatta
     5581 ttacatagta tttgtgtttc ctgtgcaggc ataaaacttt gagcaaggag aaaaatcttt
     5641 tttccggtga tggtatccat ggccatatcc accacaattt ccgcgtgacc gggacttccg
     5701 cctttaataa atacatctcc cggttgcata tcatctcgtt caacagaaac cagttcgcgc
     5761 gaaagcgaaa gcgtgccggc aaaggtgaat accttctcta gataagccca taaatcagat
     5821 gctgtattac ttgcctcggc agttttcttc catgaagttt gattgcccga taccactagt
     5881 ctgtttcccg ccatccactg cgcatattcc atgcgaaaac catttgtcag gtcgaaatgg
     5941 atgcgatcat attgccctgt ctgccacaaa taatcggcgc gcaaatgcat gacggcgtct
     6001 gcacattgat ggaggtcttt gttgccgatg ggttggtcca caacagcaat ataaatattg
     6061 ggattcatct ttatgcttcc atcataataa tgcacagaag cctcatgtgg ttttagtggg
     6121 agatggcgta ggtaatatcc gaatgaatta ctgtcaactg acaaccgaac ataacctgtt
     6181 ggtggatgaa tacgagtgga taataaacta cctgaagtat caatgatagg tgttactgaa
     6241 gagacaactt taattggtag tggagattgt tgcgcatttc
//
LOCUS       DAOQHY010000176         6268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_6782_length_6268_cov_4.813778, whole genome shotgun sequence.
ACCESSION   DAOQHY010000176 DAOQHY010000000
VERSION     DAOQHY010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6782_length_6268_cov_4.813778"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2651)
                     /locus_tag="PLL28_12230"
     CDS             complement(<1..2651)
                     /locus_tag="PLL28_12230"
                     /inference="COORDINATES: protein motif:HMM:NF014503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70136.1"
                     /translation="MKRSFIIVGNKVQDKPDLVTLDGYTTSHAIDLTGSRDGGITHEI
                     DADEDDIVEFEFQDDSFWMGTVGEIPLLIQNEASPTARDYSGPLEIPQVLSMGSDQRG
                     FLRNVILKRLHIRKPAEIITSIIVKKSAQLLDAHIQESPGLYFVDKNFRTKIINQKLT
                     EINQPYLLMIHGTAASLDGSFDKMIKNNQFGLWDSIQKQYGDRILALQHYTLSKSPVE
                     NAIEAIKWMPKNATLHIMSTSRGGLVGELIAKLSDKGNPSFKPENRQLIADAFGDANV
                     KRLEDEISSRNITVEKFIRTACPAAGTSLCSKRLDRFLTVLINGIQFATVGASAPALA
                     PFKKLVADILACKAKPDVLPGLYAMNPDSGFIKALNHPQESIGSPLYVIAGNNKISLK
                     PKAFVTILSKLIFYGKNDWIVDTKSMTRGIFRKQNIGYFLDEGDDVNHFHYFANRDTQ
                     EAIKEAVNAPTNAIASKFESFDRNLASNADRGWVFGNLEPKPVSGEKPVIVMLPGILG
                     SNLSKDGHEIYLSIDDIMAGKMTQLNMQATNVKATSLIKKFYGKFAKEFDNDYDVAVF
                     PYDWRLDLRKEADRLNDYITNILHRTGNHSIKIVAHSMGGLVVRSFIQRHANTWNAIK
                     QRSDYRVIFLGSPLGGSYLIPEIMVGSGGRIKQMANMDLIHSKNTLLEVFNKYDGLFN
                     LLPADTSIHDFTSLQTWQEMKQNFTSFNWPIPSSATVNNYKSFHTEVGNFDRSVYLDK
                     NIIYIAGKSDGTTNGFYYKDHAVDGDKLTFTETPYGDGSVTWESGIPAEIKSGDRVYY
                     VRVGHGDLANETSMFPGIKELLIAGKTSKLSKNPPQQRGEIPVSDKRDELIQDVTPEE
                     FENTMIGSTPTEPMDEEFTTPINVVLR"
     gene            complement(2961..3614)
                     /locus_tag="PLL28_12235"
     CDS             complement(2961..3614)
                     /locus_tag="PLL28_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70137.1"
                     /translation="MKKQYFIAIAVTFLLATLGMGLWLAEILFIKGWHGLQWLSGILY
                     TPYFITMIAAIAFILPFAFVNRRSIKQLLIPALLIYFTNLVFYVIGTYLCYAVYYRFL
                     IFNTRYLQNIYFDIIVYFTLFGFFYWLVTDRLIKRIKFINMLLISFAAALSVILSLAT
                     VAIIPGFGTGTDLIDAVKMGYNVFWIIFMLGISGWSIAVKKPMAQKLNQDANQIDLV"
     gene            complement(3651..4076)
                     /locus_tag="PLL28_12240"
     CDS             complement(3651..4076)
                     /locus_tag="PLL28_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNF70138.1"
                     /translation="MPAKKTKLVEIKTKATIASVDDFVNAISDEQTRNDCHAIIKMME
                     KVSGDPAVMWGPAIIGFGHRIYTSEKTGRSVDWMKIGFSPRKANISLYLSVDINKHTD
                     TLKKLGKHKTGKGCLYINKLADIDVKVLEGLIKESLKNK"
     gene            complement(4151..5005)
                     /locus_tag="PLL28_12245"
     CDS             complement(4151..5005)
                     /locus_tag="PLL28_12245"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNF70139.1"
                     /translation="MRYIALLFLLTCTINNQIIAQVDSSHANNQFTASPFTFNLNLKT
                     DLIISGTAGLSWGGTLFTTHQPLNITSLAALDTNNLIPLDRYPFSVDSAEAADWVIIS
                     DVANLASFGAPLVVGLLLLSEKNEALTYAVMYAEGFTLTYGVTQLLKEAVSRPRPYAY
                     QDNYPIEYRLSKDEWASFPSAHTSSAAFNCFFAAYMLDKMILDKQADEKLRVAIWAAA
                     SALPAYTGFARVSAARHFPTDVAAGYVLGAGLGLLIPYLHEHKAQGIAMTPLFSNEYL
                     GASIVIQR"
     gene            complement(5005..>6268)
                     /locus_tag="PLL28_12250"
     CDS             complement(5005..>6268)
                     /locus_tag="PLL28_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70140.1"
                     /translation="FINALSTLKHATVVPELKKIYNDAGDSSSLQTAVLNALSEMHTL
                     EATKAFSELLYIETPLAQKGYSLGWIFSDFSDSLKLAASLFPQLWDFTSFPEYHYPTY
                     RLLAKLYAEDSTRLKLYADHKKQVITQASAELKRNLTDDDEDDDDNSYSRYYNKHYFT
                     KLKDITDNDEKAVGNFYGTDQFAEKDEDYIGLSTNLISRLGLEDDDLNTRFDWASSLQ
                     DYYSILLTPFYDDPMVKKYYDRILRCKDKDLILSTALTMLEKGLPVPDSVWTNLQKSR
                     EYRYETIDALMEMGRKDKIDTTYGNQRMITECILYQYDFTKDEDSIQFITKTLVHTRE
                     GDGYIYFYKSKMGKDKGWSLDCTGLQPENGATYTIEPMFVHYGDVILDEEDQQKKMDE
                     IIRNIELLGRDRVSIKFDGDKSYDYYDY"
BASE COUNT         1808 a         1262 c         1209 g         1989 t
ORIGIN      
        1 catcttagca ctacatttat tggtgtagta aattcttcat ccatgggttc tgttggtgtg
       61 gaacctatca tggtgttttc aaattcttca ggtgttacat cctgaataag ttcatcgcgt
      121 ttatcggaaa cgggaatttc tcctcgctgt tgtggtgggt ttttgctgag tttggatgtt
      181 ttacctgcaa tcaacaattc cttaatcccc ggaaacatac tcgtttcatt ggcaagatct
      241 ccgtgaccga cgcgtacata ataaacacgg tcaccggatt ttatttcagc gggaatcccg
      301 ctttcccagg tcacactgcc atcgccatac ggtgtttctg taaatgtaag tttatcacca
      361 tctactgcat ggtctttata gtaaaaacca ttcgtagtac catcgctttt accggcgata
      421 taaataatgt ttttatcgag ataaactgat ctgtcaaagt tgccaacctc tgtatgaaag
      481 cttttgtaat tatttacagt agctgatgat ggtattggcc aattaaagga agtgaaattt
      541 tgtttcatct cctgccatgt ttgcagcgac gtaaaatcat gtatgcttgt atctgccggt
      601 aacaaattga acagaccatc atacttgtta aatacttcta ataaagtatt tttagaatgt
      661 atcaggtcca tatttgccat ttgcttaatc cgacctcccg agccaaccat aatttcagga
      721 atgagatagg agcctccgag tggtgagcct aagaatatca ctctgtagtc agacctttgt
      781 ttaatggcat tccacgtatt tgcatgtcgt tgaataaatg aacgcaccac aagtccgccc
      841 atgctgtgtg ccacaatttt tatactatga tttcctgtgc gatgaaggat attggtgatg
      901 taatcattta atctgtcggc ttccttgcgc aaatctaaac gccaatcgta tggaaaaact
      961 gccacatcgt aatcattatc aaattctttt gcgaattttc catagaattt tttgatcaat
     1021 gaggtggctt tgacatttgt agcctgcatg ttaagttgtg tcattttacc cgccatgata
     1081 tcatcaatgc tgaggtatat ctcgtgacca tccttagaca ggttgctacc taatataccc
     1141 ggaagcatta cgatgacagg tttctcacca ctgacaggct tcggttccag gttgccgaat
     1201 acccatccgc gatcggcatt ggaagcaaga ttgcgatcaa aggattcaaa ttttgaggcg
     1261 atggcatttg tcggtgcatt tacagcctcc ttaatagcct cttgtgtatc gcgatttgca
     1321 aagtaatgga aatgatttac gtcatcacct tcatccaaaa agtaaccaat attttgtttc
     1381 ctgaaaattc cgcgagtcat actctttgtg tcaacgatcc aatcattttt accgtaaaat
     1441 atcagtttgg acagtatggt aacgaatgct tttggtttaa gcgatatttt gttgttacct
     1501 gctataacat atagtggcga tccgattgat tcctgaggat gatttaatgc tttaataaat
     1561 cctgaatccg gattcatggc atataatccc ggaagcacat caggttttgc cttgcaagct
     1621 aaaatatcgg caaccaattt tttaaatggc gcgagggcag gagcagaggc gcctactgta
     1681 gcgaattgta tgccattgat caatacggtt aaaaatctgt cgaggcgctt agaacataag
     1741 cttgttccgg cagcagggca tgcggtgcgg atgaactttt ctaccgtaat atttctgctg
     1801 ctaatctcat cttccaaacg ttttacattt gcatcaccga atgcatcagc tattaattgt
     1861 ctgttttccg gtttgaagga tggatttcct ttatcactca atttcgcgat gagttcgcca
     1921 acaagtccgc cgcgactggt tgacataata tgcagcgtag cattttttgg catccatttg
     1981 atagcttcga ttgcattttc taccggtgat ttagaaagtg tgtaatgttg aagtgcaagg
     2041 atgcgatcgc catattgttt ttgaatacta tcccataaac caaattgatt gtttttgatc
     2101 attttatcaa atgaaccgtc tagtgaagca gcggttccat ggatcataag taaatatggc
     2161 tgatttatct ctgtcagctt ttggttaatg attttcgttc tgaagttttt gtcgacaaaa
     2221 tataatcccg gtgattcctg aatatgggca tccagcaatt gtgccgattt tttcacgata
     2281 atactcgtaa taatctcagc cggtttgcga atgtgcaaac gttttaaaat aacattgcgt
     2341 agaaacccac gctgatcact ccccatagag agcacctgag gaatttccaa gggaccggaa
     2401 taatctctgg cagtagggga tgcctcattt tgaattaata aaggtatttc accaacagtt
     2461 cccatccaga aactatcatc ctgaaattca aattcaacaa tgtcgtcttc atcggcatca
     2521 atttcatgtg taatgcctcc atcccgactt ccagtcagat caatggcatg cgaagtggta
     2581 tatccgtcta aagtaaccaa gtcgggttta tcctgcacct tattacctac tatgataaat
     2641 gaacgtttca taaaatatat gtttatagaa gattgtatcg atttgttaaa aatgaatact
     2701 tacatattca tcgcgacgga gtatttcaag ttcaatcgcg gtaaacctat ccaaggtagt
     2761 atatagcgcc tttatttgca aggatttttc gcaaattacc ggaaaaccgt taatgtcaaa
     2821 aatgtctcat tttgatgcaa attttgcatg attttgtgac ataggtgttg gctgaaagct
     2881 agtatactct tgtgttggaa tatgtcacag aaaggttgaa aagtgcggta tcagactgag
     2941 ctatttgatt aaacaggtga ttaaaccaaa tcaatttgat tagcgtcttg attcaacttt
     3001 tgtgccatag gtttcttgac tgcaattgac caacctgata ttccgagcat aaagatgatc
     3061 caaaacacgt tatatcccat ttttacagca tctatcaggt cagtgccagt tccaaatccg
     3121 ggaattattg caacagttgc aaggcttagt attactgaga gtgcagcggc aaagctgatc
     3181 agtaacatat taataaattt gatccttttg atcagcctgt cagttaccag ccaataaaaa
     3241 aatccaaaca gggtgaaata tactatgatg tcaaaatata tattctggag atatctggta
     3301 ttaaaaatga gaaacctgta atacacggca taacacaagt atgtgccaat aacataaaaa
     3361 actaagttgg taaaatagat cagtagtgcg ggaatcagta attgtttaat agaacgacga
     3421 ttaacaaatg caaaaggaag aataaaggcg attgcggcaa tcatggtgat aaaatacggg
     3481 gtgtacaaaa tacctgaaag ccattgcaag ccatgccagc cttttataaa taggatttcc
     3541 gctagccaga ggcccatccc caaagtagca agaagaaaag ttactgcgat tgctatgaaa
     3601 tactgttttt tcaatttagg gttgatgaaa aatcaagaac aaaatcctta ttacttattt
     3661 ttcaatgatt ctttaatcaa cccctccaat acctttacat caatatcagc taatttattg
     3721 atatataaac atcctttgcc ggttttatgc ttacccagtt tttttagggt gtctgtatgt
     3781 ttgttgatgt caacactcaa atacaaagaa atatttgcct tcctgggcga aaacccgatc
     3841 ttcatccaat ctactgagcg accagttttt tcgctggtgt atattctgtg tccaaatccg
     3901 atgattgccg gtccccacat tacagccggg tcgccactta ccttttccat catcttgatg
     3961 atagcgtggc agtcgttgcg tgtttgttcg tcactgatgg cattcacaaa atcatctaca
     4021 ctggctatag tggcttttgt ttttatttct acaagcttgg ttttttttgc aggcatccgg
     4081 atattatttt attcaaatgt acataaatca aatccgaaat cccaataagt ttaaatgctc
     4141 gaatgctttt ttagcgctgt ataacaatgc ttgcgcccaa atattcatta ctgaaaagtg
     4201 gagtcatcgc aattccttga gccttgtgtt catgcagata cggtattaac aagcctagtc
     4261 cggctcccaa aacataccct gccgcaacat ctgttggaaa atgcctggca gcgcttacac
     4321 gagcaaatcc tgtataggca ggcagagctg atgctgctgc ccaaatagca acgcgcaatt
     4381 tttcatctgc ttgtttgtcg agtatcattt tatccaacat ataagctgcg aaaaaacagt
     4441 taaaggcagc agaagatgta tgtgcacttg gaaaagaggc ccattcgtct ttactcagtc
     4501 tgtattcaat agggtaatta tcctggtagg cgtaaggccg cggtctgctt acagcttctt
     4561 tcaataattg tgttacacca tatgtcaatg taaatccttc cgcatacatg acggcatagg
     4621 tcaaagcttc gtttttttct gatagtaaca ataatccaac tacaagaggt gcaccaaagg
     4681 atgcgagatt tgcaacatcg cttataataa cccaatctgc ggcttctgca gaatctacag
     4741 aaaatgggta tcgatctaat ggaattaaat tgttggtatc taaggcggct aaagaagtaa
     4801 tattcaaagg ttggtgagtg gtgaaaagcg ttcctcccca acttaatccc gcagtgcctg
     4861 aaatgatcag gtcggtttta aggtttaaat taaatgtaaa tggtgatgca gtaaattgat
     4921 tatttgcatg cgaactatca acctgtgcaa ttatttgatt gttaattgta caggtcaata
     4981 gaaataataa tgcaatgtat cgcattaata gtcgtaataa tcataactct tatcgccatc
     5041 gaatttgata ctcacccgat cgcgacctaa taattcaata ttgcgaatga tctcatccat
     5101 tttcttttgc tgatcttctt catccagaat cacatcgcca taatgcacaa acatgggctc
     5161 aatggtgtat gtggcgccat tttcaggctg taatccggtg caatccaggc tccagccttt
     5221 gtcttttccc atcttgctct tatagaaata aatatacccg tcgccttctc gtgtgtgaac
     5281 caatgtttta gtgatgaatt gaatactgtc ctcgtctttc gtgaaatcat attgatacag
     5341 tatacattct gttatcatgc gttggttgcc gtaagtagta tcaattttat ccttgcggcc
     5401 catctccatt aaagcatcaa tagtttcata gcgatattct ctgctctttt gcaaattggt
     5461 ccaaacacta tcaggaaccg gtaatccttt ttccaacatg gtaagtgcag tgctgagaat
     5521 taaatcttta tccttacaac gcaatatgcg atcataatac tttttcacca tcggatcatc
     5581 atagaatgga gtaagcaata tggaataata atcttgtaaa gaacttgccc agtcgaatcg
     5641 cgtgttgagg tcatcatctt ctaacccaag gcgactgata agattggtgc tcaagccaat
     5701 ataatcttca tctttttcgg caaactgatc ggtgccgtaa aaattgccta ctgctttttc
     5761 atcgttatcg gtgatgtctt tcagcttggt gaagtaatgc ttgttataat accttgagta
     5821 actattgtca tcatcatctt catcatcatc cgtcaggttg cgctttaatt ctgcagacgc
     5881 ctgtgtgatc acttgctttt tgtgatcggc atacaattta agacgcgtgc tgtcttcagc
     5941 atataatttt gccaacaatc gataagtggg gtaatgatat tccgggaaac tggtgaaatc
     6001 ccataattgc gggaacaggc tagctgccag tttcaatgaa tcagaaaaat ccgagaatat
     6061 ccatccaaga gaatatcctt tttgtgccaa tggggtttcg atatataaca attcactaaa
     6121 tgccttagtg gcctccaggg tatgcatctc cgaaagggca ttcagtacgg cggtctgtaa
     6181 tgaagaacta tcaccggcat cgttataaat tttctttaat tccggaacaa cagtagcatg
     6241 tttcaaggtg gataaagcat taataaat
//
LOCUS       DAOQHY010000177         6255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_6811_length_6255_cov_4.305484, whole genome shotgun sequence.
ACCESSION   DAOQHY010000177 DAOQHY010000000
VERSION     DAOQHY010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6811_length_6255_cov_4.305484"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PLL28_12255"
     CDS             complement(<1..695)
                     /locus_tag="PLL28_12255"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNF70141.1"
                     /translation="MSIDQFRLLSKYDVAVPRYTSYPTVPSWDFQTIQGDNWKSAVIR
                     SFKQENQELCIYIHLPFCEELCTYCACNKRITKNHAVEIPYLESVLAEWEMYRALLAD
                     TPVIREIHLGGGTPTFFSPEHLKQLINGITKHAIVANDHAFSVEVHPNYTNEQHLVAL
                     QEVGFNRISLGVQDFDPVVQFIINRPQTFEQTQHVIKTARALGFTGVNIDLIYGLPKQ
                     TNDSIKLTMEKVK"
     gene            973..3582
                     /gene="clpB"
                     /locus_tag="PLL28_12260"
     CDS             973..3582
                     /gene="clpB"
                     /locus_tag="PLL28_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HNF70142.1"
                     /translation="MNLQQFTIKAQELIQQAQQMAMQMDHQYIMPEHILKALLSDDEV
                     AGYLLKKLQANPVYIQQKLDEQLAAVAKVASGQGGQYISREANLVIAKSQTFLKEFND
                     EFVSVEHLLLGLLATNDAVSKLLKSQGVNEKDLKAAIRDFRKGSTMHSQSGENQINAL
                     NKYAINLNERASSGKLDPVIGRDEEIRRVLHILSRRTKNNPILVGDPGVGKTAIAEGI
                     AHRIVNGDVPENLKSKTIFSLDMGALIAGAKYKGEFEERLKGVVKEVIAADGQIILFI
                     DEIHTLVGAGGGGEGAMDAANILKPALARGELRAIGATTLNEYQKYFEKDKALERRFQ
                     KVMVDEPGFEDAVSILRGLKERYENHHKVRIKDEAIIASVELSSRYISDRKLPDKAID
                     LIDEAAAKMRLEMDSLPEELDEIERRIRQLEIEREAIKREKDDKKVEELSKAIADLSE
                     ERNAIKAKWQQEKEVVEKIQQSKIDIEQYKLQAEQAEREGDYGRVAELRYGKIKEAEA
                     ASIAYEQELKKMDAGKRLMKEEVDAEDIAEIVAKWTGIPVAKMLESDRMKLLRLEEEL
                     HKRVVGQDEAIAAVSDAIRRSRAGLQDPKRPIGSFIFLGTTGVGKTELAKAISDFLFN
                     DEHAMTRIDMSEYQERHSVSRLIGAPPGYVGYDEGGQLTEAVRRKPYSVVLLDEIEKA
                     HPDVFNILLQVLDDGRLTDNKGRTVDFKNTIIIMTSNIGSHIIQERFEGVNEDNLLAV
                     TATTRIEVMEMLRKTIRPEFLNRIDEIIMFKPLLRSEIRGVVEIQLHLLADLLAERDI
                     KLKFTPALITWLADEGFDPQFGARPLKRVIQKEIVNTLSKRILAGEVTAGSGAEIEVD
                     VAQGDVVFRNVVG"
     gene            3696..3869
                     /locus_tag="PLL28_12265"
     CDS             3696..3869
                     /locus_tag="PLL28_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70143.1"
                     /translation="MENTKKEKYEIKVNMKFLLLGLLFSAATVWMVFNLSQFFWIGLP
                     FSFTFLAKSIDAL"
     gene            complement(3993..5450)
                     /locus_tag="PLL28_12270"
     CDS             complement(3993..5450)
                     /locus_tag="PLL28_12270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNF70144.1"
                     /translation="MLLTFVIIYMVATIAIGVLASRLVKGSGDFVQAGRRLPPFFNAA
                     ALFALWFGSETVFGASSAFLEGGLLNVIEDPFGASLCLFLFGFFFVRKLYRMNLLTIG
                     DLFRIKYGQNVELISSFFMLLTFFGYIAAQYVALGIILQVVLLQTGINMSLPTAIILC
                     SIIVTAYTMIGGMWAVSITDFIQSILIVIGLIIVVIFVANEAGGVSQVIANAPENTFK
                     FLPNAEPIDITNWIGAWMVIGLGYIPSQDVFQRANSARSEKAAVNSTYLGAIMYLLIA
                     MLPLFIALAARVLYPDQDFSDSQSVLPHIVLDHTPLWIQILFFGSLLSAVLSTCSGSL
                     LAPASILAENFIKPLYKSNGKREMTDRKFLIVLRLSVVAIAFISFLITLPRQNIYELV
                     GESATFGMVSLLAPMTAGLYWKNINTQGALLSMFGGLVVYVFFQYFYPISIEAFIPAT
                     IVSFICMYAGRFIDEKKSPRYASTLANRFTKFFKD"
     gene            5608..>6255
                     /locus_tag="PLL28_12275"
     CDS             5608..>6255
                     /locus_tag="PLL28_12275"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70145.1"
                     /translation="MRKIILPFMLLAFAGTSCSHEPETSDAASKTDTMQFNDPHSLAQ
                     PTVAVGTHLDWHATVDFNTHTISGTAIWTLKSSPDAKEVIFDTRGLTISKVTTDGDNV
                     AAFTLGDTIPGQPWMGQALHIPISASTKTVHISYSSSPDAAALQWLSAEQTRDKKFPF
                     LFTQSQAILARTWVPCQDGPGVRFTFNAEVQVPTGMMALMSAENPQSVSADGIYRF"
BASE COUNT         1982 a         1260 c         1357 g         1656 t
ORIGIN      
        1 tccttcacct tttccatcgt caatttgata ctgtcgttgg tctgtttagg taacccgtat
       61 atcaagtcga tattcacgcc ggtaaagcct aaagctctgg ccgttttaat cacatgttgt
      121 gtttgctcaa atgtttgagg gcgattaata ataaattgca caacagggtc aaaatcctga
      181 acacctaaac tgatgcgatt aaatccaacc tcctgcaatg ctacaagatg ttgttcattt
      241 gtataatttg gatgtacttc aacactaaag gcatggtcat tcgcaacaat tgcatgttta
      301 gtaatgccat tgatcaattg tttcaaatgc tccggtgaaa agaaagtggg tgtgcctcct
      361 cctaaatgta tttcacgaat aacaggagtg tctgccaaca atgcacgata catctcccat
      421 tctgcaagca ctgattccaa atagggtatt tcaactgcat gattttttgt gatgcgttta
      481 ttgcatgcac aataggtgca taattcctcg caaaaaggta ggtgtatata tatacacagt
      541 tcttggttct cttgtttgaa tgagcgaatt acagctgatt tccaattatc accttgaatt
      601 gtttgaaaat cccacgacgg cactgtagga taactggtgt accgaggtac cgcaacatca
      661 tattttgaaa gtaacctgaa ttgatctatc gacatgaatg attatcggtc tttatgagga
      721 atgcattccc tcaatcattg cacaaagata caaccaccgg tctattagat tgttaaagaa
      781 ttgtcattca aaacctgggt gaaaggattt ttgttgtatg ctattgacaa cttgaccttt
      841 atgcttgaga tggcctgaca gcctgtcctt tggggctatt gaaacaggac atattggcgt
      901 aaaagttttg gtattcagac gaagtatgtt ttttgctata tgctttgcat tatcccttaa
      961 actaagactg atatgaactt acaacaattt actatcaaag cacaggaatt gatccagcag
     1021 gcacagcaaa tggccatgca aatggatcat caatatatta tgcccgagca tatcctgaaa
     1081 gcactgttat ctgatgatga ggttgcagga tatctgctta agaaattaca ggctaaccca
     1141 gtttatattc aacaaaaatt agacgagcag ttagctgcag ttgcaaaggt ggcttcggga
     1201 caaggaggtc agtatatctc tcgcgaagcg aatttggtta ttgcaaagag tcaaacattt
     1261 ttgaaagaat tcaacgatga atttgtttct gttgaacacc ttttgttagg attactggca
     1321 actaacgatg ccgtgtcaaa acttttaaaa tctcaaggtg taaacgaaaa agacttaaaa
     1381 gccgccattc gggatttcag aaaagggtca accatgcatt ctcagagcgg cgagaatcaa
     1441 ataaacgcac tgaataaata tgcaataaat cttaacgagc gagcatcaag cggaaaactg
     1501 gatcctgtaa tcggacgtga tgaagaaatc cgtcgtgtgt tgcatattct ttcccgacgc
     1561 acaaaaaata atcctatttt ggtaggtgat ccgggtgtcg gaaaaactgc catagccgaa
     1621 ggaatagcac atcggattgt aaacggtgat gtgcctgaaa atctgaaatc aaaaacgata
     1681 ttttccctcg atatgggagc attaatcgcc ggtgcaaaat ataagggtga atttgaagag
     1741 cgattgaaag gcgtggtgaa agaagtgatt gccgctgatg gtcagattat tttgtttatt
     1801 gatgaaatac atacccttgt aggtgccgga ggtggtggtg aaggagctat ggatgctgca
     1861 aacattttga aaccggcatt ggcaagagga gaattacgtg ctattggcgc tactaccctg
     1921 aatgaatatc aaaagtattt tgaaaaggat aaagcacttg aacgaaggtt ccagaaagta
     1981 atggttgatg aacccggatt tgaagacgcc gtttccatcc tccgcggatt aaaagagcgt
     2041 tatgaaaatc accataaagt gcgcattaaa gatgaagcga ttattgcatc tgttgaatta
     2101 agttcgcgat atatttcaga tcgtaaatta ccggacaagg caatcgactt gattgatgaa
     2161 gctgcagcaa aaatgcgcct cgaaatggac agtctcccgg aggaattgga tgagatagaa
     2221 agacgcatcc gccaattgga aattgaacgt gaggcaatca aacgtgaaaa agatgacaag
     2281 aaagtagaag aattgtccaa ggccattgcc gatttatctg aggagcgaaa tgcaataaaa
     2341 gcaaagtggc agcaagagaa ggaagtagtg gaaaaaatac agcaaagtaa aatcgatatc
     2401 gaacaatata aactgcaggc cgagcaagca gaacgcgaag gtgattatgg cagggttgct
     2461 gaattgcgat acgggaaaat taaagaggcg gaagctgctt ccattgccta tgagcaggaa
     2521 ttgaaaaaaa tggatgccgg taaacgttta atgaaagagg aggttgatgc cgaagatatt
     2581 gccgagatcg ttgctaaatg gacaggaatc ccggttgcaa aaatgctcga aagcgacaga
     2641 atgaagttat tgcgacttga agaagaattg cacaaaaggg tagtggggca agatgaagct
     2701 atagctgcgg tatcagatgc tatcagacgc agtagagcag gactgcaaga tccgaaaagg
     2761 ccaataggtt catttatttt tctcggaact acaggtgtag gtaaaactga attggcaaaa
     2821 gccatttccg acttcttatt caacgatgag cacgcaatga cacgcatcga catgagcgaa
     2881 tatcaggaac gtcatagtgt gagcagattg attggcgctc ctccgggata tgttggctat
     2941 gatgaaggag gtcagcttac agaagccgtt cgtcgaaaac catatagcgt ggtgttattg
     3001 gatgaaatag aaaaagcgca tcccgatgta tttaatatcc tgttgcaggt attggatgat
     3061 ggtcggctta ccgataataa aggtcgcact gttgatttta aaaacactat catcatcatg
     3121 accagcaata tcggttcaca tatcattcag gagcgattcg aaggtgtgaa tgaagataat
     3181 ttgctggcag taacggcaac cacaagaatt gaagtaatgg aaatgcttcg caaaaccatc
     3241 agaccggaat ttcttaatcg tatcgatgag attatcatgt tcaaaccgtt gttgcgaagc
     3301 gaaatcagag gcgtagttga aattcaattg catttgttgg ccgatttgct tgccgaaagg
     3361 gatataaaac tgaaattcac tcctgcattg ataacctggc tcgccgatga aggttttgac
     3421 cctcaatttg gagcccgacc actgaaaaga gtcatccaga aggagattgt caataccctc
     3481 tcaaaacgga tattggcggg agaggtaact gccggcagcg gcgccgaaat agaggtggat
     3541 gtagcgcagg gcgatgtggt tttccgtaat gttgttgggt aaaatcacct gccaaatgcc
     3601 cgattacata gggtttgggc aatttgtggg ttttccacca ccaaggtttt gaaatccgat
     3661 tttgatgcgt aactttgtcg ggtcaattta cagctatgga aaataccaaa aaagaaaaat
     3721 acgagatcaa ggtaaatatg aagttcctct tgttaggtct tctttttagc gccgctacag
     3781 tatggatggt gttcaaccta agccagtttt tctggatagg attgccattc tcgttcacct
     3841 tcctcgctaa gtcaatcgac gcattataaa ttaagttacc ttatcaaaag atataggcaa
     3901 ttccttaacg ggagttgcct ttttttttat gccacaaata ccttattaat acacaaacta
     3961 atcagcaaaa agttattcgg agagcgtcta aatcaatcct taaaaaattt agtaaacctg
     4021 ttggcaaggg tcgatgcata gcgcggtgat ttcttctcat cgataaatct gccggcatac
     4081 atacaaatga agctgacaat ggttgcagga ataaatgctt ctatgctgat aggataaaaa
     4141 tattgaaaga aaacatatac aaccaggccg ccgaacatac tcaatagtgc accctgcgtg
     4201 ttgatgtttt tccagtataa tccggctgtc attggtgcaa gtaaactcac catgccaaag
     4261 gtggcacttt cgccaaccaa ttcatatatg ttttgtcgag gtaacgtaat aaggaaagaa
     4321 ataaatgcta ttgcgacaac actcaatcgc aatacgataa gaaatttcct atcagtcatt
     4381 tctcgcttcc cattcgattt gtatagtggc tttataaaat tctctgctaa tatgcttgca
     4441 ggagccaaca atgaaccgct gcatgtactt aatactgccg ataataagga accaaaaaat
     4501 aatatctgaa tccatagtgg cgtatggtct aaaacaatat gcggaagcac agattgtgaa
     4561 tctgaaaaat cctgatcagg ataaagaact ctcgctgcaa gtgcaataaa caatggcaac
     4621 atggcaataa gcaaatacat gatggcaccc aggtaagttg aatttactgc agctttctca
     4681 ctgcgcgccg aatttgctct ttgaaaaaca tcttgcgatg gaatatagcc caatccgatt
     4741 accatccatg caccaatcca attagtaatg tcaatcggct cggcattcgg tagaaacttg
     4801 aacgtatttt caggtgcgtt ggcaattacc tgcgatacac caccggcctc atttgccaca
     4861 aaaattacca caatgatgag tccaattacg atgagtatac tttgaataaa atcagtaatc
     4921 gaaaccgccc acataccgcc aatcatggta tacgcagtta ctattattga acagagaatg
     4981 attgcagttg gcaacgacat gttgattcct gtttgcaata agaccacttg taaaataatc
     5041 cccaacgcta catactgagc agctatatat ccaaagaatg tcagcaacat aaagaagctg
     5101 gaaatcaact caacattttg accatatttt atgcgaaaca gatcaccaat cgtaagcaga
     5161 ttcattctgt aaagctttct tacgaagaaa aatccaaata ggaaaaggca tagcgatgcc
     5221 ccgaaaggat cttctatcac gttgagcaac ccaccctcaa gaaatgcact ggaagcacca
     5281 aacaccgttt cgctgccaaa ccatagggca aacagcgctg cggcattgaa aaatggaggt
     5341 aatcgccttc ccgcctgaac gaaatcgccg gagcctttta ccaatctact ggctaataca
     5401 ccgatggcaa tggtggccac catgtagatg atcacaaagg tgagtaacat ctctctggga
     5461 agggtatctg gttgtagtga aaaattttag cggtcaaaaa taaaaaaatc gtcgaaactg
     5521 cccaatagcc taaataaaca tattgttgat ttttctcacc tgtgaaagcg cctaaggaat
     5581 ccgtaaattt gccaaatact tcaaggcatg cggaaaatca ttctaccttt tatgttactg
     5641 gcttttgcgg gaacttcctg cagccatgaa cccgaaacga gtgatgctgc atccaaaacc
     5701 gatactatgc aattcaacga tccacactcc ttagcacaac ccactgtggc tgtcggcacg
     5761 cacctcgatt ggcacgctac agtagatttt aacacacata ctatttcagg aaccgcaatc
     5821 tggaccctca aatcatctcc cgatgccaaa gaggtcatct tcgatacgcg cggtttaacc
     5881 atctctaagg ttaccactga tggcgataat gttgccgcat tcacgcttgg cgataccatt
     5941 ccgggacaac cgtggatggg tcaggctttg catattccca tttcggcatc aacaaaaact
     6001 gttcatatct cctatagctc atcgccagat gcagctgctt tgcaatggct ctcggcagaa
     6061 caaacacgcg ataagaaatt tccctttctg ttcactcaat cgcaagccat ccttgccaga
     6121 acttgggtgc cttgtcagga tggaccgggt gtccgcttta cttttaatgc cgaagtgcaa
     6181 gttccaacag gaatgatggc cttaatgagc gccgaaaacc cacaaagtgt aagtgccgat
     6241 ggcatatatc gcttc
//
LOCUS       DAOQHY010000178         6254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_6813_length_6254_cov_4.315212, whole genome shotgun sequence.
ACCESSION   DAOQHY010000178 DAOQHY010000000
VERSION     DAOQHY010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6813_length_6254_cov_4.315212"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PLL28_12280"
     CDS             complement(<1..741)
                     /locus_tag="PLL28_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNF70146.1"
                     /translation="MDRSKITKETYLNWYELMYKMRRFEEKAGQLYGQQKIRGFCHLY
                     IGQEAVAAGTISAIRHEDPIITAYRDHGLAIAKGISCNAAMAELFGKVTGVTQGKGGS
                     MHFFSKENYFFGGHGIVGAQIPLGAGIAFSEKYLGTQNVCACFFGDGAARQGALHETF
                     NMAMLWKLPVIFICENNEYAMGTSVERSSNVSDISKLALGYDMPSMQVDGMRCESVHD
                     AVAAAAARGRRGEGPTFLEIKTYRYKGHS"
     gene            802..1905
                     /gene="recF"
                     /locus_tag="PLL28_12285"
     CDS             802..1905
                     /gene="recF"
                     /locus_tag="PLL28_12285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HNF70147.1"
                     /translation="MLLRSIRLLHFKNYASAEAVFSHAFTLINGLNGSGKTNLIDAVY
                     YLCLCRSYFTRSDSSVLQHDAPYFRIDGTFSNPEGSLLITCKYLTAKKEVLRNEVPYD
                     RISDHIGNFPVAMIAPDDINIINEGSEERRRFLDAAISQLDREYLHNLILYNKYLAQR
                     NALFKQALGGGIRPDATLVGVLNHSLALHGNAIYRARRAYLEAFTPIFRDMYSRISGG
                     TEEADISYKSQLEEHDHLHLLLHSERDDRAAGRTTTGIHKDDLYLSVKQYPVRDTGSQ
                     GQIKSFLIALKLAQCYMMFRSEGKQSILLLDDIFEKLDKQRLEVLFNMLQDEVFCQVF
                     ITDADTKRSMEFCKSHLPEFGHIHVNNGKIEYL"
     gene            1912..2202
                     /locus_tag="PLL28_12290"
     CDS             1912..2202
                     /locus_tag="PLL28_12290"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNF70148.1"
                     /translation="MKKDNQLSIGDAIKQLFHSIRIEDKVAEVRIKELWPSIMGASIN
                     RYTGEIYFNKGILTIYINSAPLKQDLQFSKQEIIERVNEELQENLVREVIIR"
     gene            2258..3124
                     /locus_tag="PLL28_12295"
     CDS             2258..3124
                     /locus_tag="PLL28_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70149.1"
                     /translation="MELFVRGRWGIWLFNLCGSAGTKWREDVTYDANGNILTYFRNGN
                     TSTMDNLSYVYNTGNNQLKRVDDAVSSGTYSVDIDDQSGTNYEYDALGNLVKDVAEEI
                     DTILWTLTGKVDSISRTGASTKDELSFLYDPMGNRVGKIVYEDDGDILKTWYIRDAQG
                     NIMSTYTEKEDSLWWTEVDLYGSSRLGVIYPDSLVYPYTYTMPGDTFSFFNIAGNRRY
                     EITNHLGNVLATITDRRIPQEVGTPNGIAEYYLADIASVQDYYPFGMVTPGRSYSDGS
                     GYRFDFYAGAGR"
     gene            3078..3959
                     /locus_tag="PLL28_12300"
     CDS             3078..3959
                     /locus_tag="PLL28_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70150.1"
                     /translation="MEVAIGLISMQEQVDEVYGNGNLNTALFWEYDLRLGRRWNIDFD
                     SEIYESPYLAFGSNPIGFKDLLGNKKDWYKDDQGIMQFDPTVQSSADLNGEGEPKGVY
                     VGNYSEEKTANGIAYYRHDGSIYYTNQTDGYNRIWNNSEPRNREQSGVILNEGVLVFP
                     DYKNNSDTGTPDVYGYSFSNGLMYDPVSKDQGKSTTQYPVVTFIHSHFAGLDPGPSYY
                     SKYGYGDLGYAAENFPNKTIIVLAWDNKVYGLISGPPPDDPTKWEYQVIDYTHISVTK
                     LLEGYDFSTFMKKINKF"
     gene            4056..4484
                     /locus_tag="PLL28_12305"
     CDS             4056..4484
                     /locus_tag="PLL28_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70151.1"
                     /translation="MIVKKNTIFDDKFLSILDQYEESTNCKICINIIYIDKIEPHKTI
                     ITIKSIGANLDYLNNNPPLMYFYHKDIVYFVFTGIEDYFKPINDYSGLFNIQDNEKCA
                     SRTWQVIDSMGGYRILKQIGYPFFPLPSKFADADSANLDN"
     gene            4549..5628
                     /locus_tag="PLL28_12310"
     CDS             4549..5628
                     /locus_tag="PLL28_12310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HNF70152.1"
                     /translation="MHSGGLYNFEIFKSYYPFGMVTPGRSYSAGSGYRFGFNGKESDD
                     QLYGENNGYDLGLRIYNPRLGRFLSLDSKSREYPWQTPYAYFFNNPVAVIDVLGAGAP
                     DGEKELSEGDGATPTQYTIKPKDNFWILENKLGLPHGNLQKWNPTLDPNNLQIGTVIN
                     TSDPYYYDIENAVEFSVLPFDGGDFTPRSFIPAQYDGMPTNLWMIYSEFVKGTGPVNS
                     IFIENPLITGQLASSNGVNEFRNFMYSKFDGSLSKMQEYASNHNRALVTDFDPYGSFY
                     PWDEIGNGVMQFIGSCSVDAFLSYDNKTIIYVVTDSKSLYSLILHSTNRLDYERTKQG
                     DAYSNTYQKYIWFETFNSQYGPENK"
     gene            5609..6013
                     /locus_tag="PLL28_12315"
     CDS             5609..6013
                     /locus_tag="PLL28_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70153.1"
                     /translation="MAQKTSKILRFLIAFLVVSILIGCNKSINIDGNFYVQKDSLRQK
                     YILRYKGENIFPNEFVNKVGYNSDWIVANTSSFQENITSSKYYIINKQNYAAQLGETK
                     NLGVMGPFDSINYEETLSELHLQNECKFIISF"
BASE COUNT         1964 a         1207 c         1305 g         1778 t
ORIGIN      
        1 cgaatggcct ttatagcggt atgtcttaat ctccaggaag gtcgggcctt caccacgcct
       61 gcctcttgcg gcagcagcgg ccacggcatc atgcaccgat tcgcatcgca tcccatcaac
      121 ctgcatggat ggcatatcat aaccaagtgc cagcttgctg atatcagaaa cattgctgga
      181 ccgttccacg gaagttccca tcgcgtattc attattctca cagataaaga tcaccggtaa
      241 cttccacagc atagccatat taaaagtttc atgcagcgcc ccctgtcttg cggcgccatc
      301 gccgaaaaaa caggcacaga cattctgtgt accgaggtat ttttctgaaa acgcgatgcc
      361 tgcaccaagc ggaatctgtg caccaacaat accatgtccg ccaaagaaat agttctcctt
      421 gctgaaaaag tgcatagaac cacccttacc ttgggtaacc cctgtcacct ttccgaaaag
      481 ttccgccatc gccgcattac aagaaatgcc ttttgcaatt gctaatccgt ggtcacggta
      541 agcagtgata atagggtctt catgtcgtat cgcactaatc gtaccggcag ctacagcttc
      601 ctgtccgatg tagagatgac aaaacccgcg aatcttttgt tgaccgtata attgccccgc
      661 tttctcttca aagcgacgca ttttatacat cagttcgtac cagttgaggt aggtttcttt
      721 ggttatcttt gagcgatcca ttattcgtgc cgaaaaatcg aagggcaaag ttacgaaaag
      781 gagaccacaa tcaaaacaac cgtgttactg cgcagtatac gcctcctgca ctttaaaaac
      841 tatgcttctg ccgaggccgt tttttcgcat gcattcaccc ttatcaacgg tctgaacggc
      901 agtggtaaaa caaacctgat cgacgccgtt tactacctct gcctttgtcg tagctacttc
      961 acccgttccg atagcagtgt gttgcaacat gacgccccgt atttcaggat tgacggcact
     1021 ttcagcaatc cggaaggcag cctcctcatt acctgcaaat acctgacggc gaaaaaagaa
     1081 gtgctgcgaa atgaggtgcc atacgaccgc atttccgacc atatcggcaa tttcccggtg
     1141 gccatgatag cgccggacga catcaacatc attaacgaag ggagcgagga gcgacgcagg
     1201 tttctcgacg ccgctatatc gcaactcgat cgcgaatacc tccacaatct cattctttac
     1261 aacaagtatc tcgctcagcg aaacgctttg tttaagcaag ctttgggggg aggaatcagg
     1321 cctgatgcta cattggtggg agtgctcaac cacagtctcg ccttacatgg caatgcaatc
     1381 taccgcgcaa gacgggctta tctcgaagca tttacaccca ttttccgcga tatgtattcc
     1441 cgcatttccg gcggtacgga ggaagctgat atcagttata agtcgcaact cgaagaacat
     1501 gatcacctgc acctgctgtt gcacagtgaa cgtgacgacc gggctgcagg tcgcactaca
     1561 accggtattc ataaggacga tctctatctg agtgtcaagc aatatccggt gcgcgatacc
     1621 ggaagccagg gtcaaataaa atcctttctt atcgccctga aactggctca atgttatatg
     1681 atgtttcgca gtgaaggcaa acaaagtata ctcttgctcg acgatatctt cgaaaaactc
     1741 gacaagcagc gattggaagt attgttcaac atgttgcagg atgaagtgtt ttgtcaggta
     1801 ttcatcaccg atgcagatac caaacgaagc atggaatttt gtaaatcgca cctgccggaa
     1861 ttcggacata tacatgtaaa caacggtaaa atcgagtacc tttaatccaa gatgaaaaag
     1921 gacaatcaac tcagtatcgg tgatgccatt aaacagctgt ttcacagcat ccgcattgag
     1981 gataaagtgg ctgaagtacg cattaaggag ttgtggcctt ccataatggg tgcatccatt
     2041 aatcgataca ccggcgagat ttatttcaac aaaggcatac tcaccattta catcaactcc
     2101 gcacctttga aacaagacct ccaattttct aaacaggaga tcatcgaaag ggtgaatgaa
     2161 gaactccaag aaaatctagt tcgagaagta ataattagat gaaggaaaaa ggaaaaagga
     2221 gagaggagaa aggagagagg agaaaggaaa ttctggtgtg gaattatttg ttcgaggtcg
     2281 gtgggggatt tggttgttca acctctgcgg ctctgcggga actaagtggc gtgaagatgt
     2341 tacttatgat gccaacggta acattctgac ttatttccgt aatggcaaca cctcgacaat
     2401 ggacaacctg agttacgtat ataacacggg taataaccag ttaaagcgtg tagatgatgc
     2461 cgtctcatcc ggtacgtata gcgtagacat tgatgaccaa agcggcacta attacgaata
     2521 tgatgctttg ggtaaccttg ttaaagatgt ggcagaggaa atcgatacca ttttgtggac
     2581 attaacaggc aaagtggata gtatttcgcg aacaggtgca agtactaagg atgaattaag
     2641 cttcctgtat gaccctatgg gaaaccgggt aggtaagatt gtgtatgaag acgatggcga
     2701 catactgaaa acatggtata ttcgcgatgc acagggtaat atcatgtcaa cttatacaga
     2761 aaaggaagac tcattgtggt ggacggaagt agatctctat ggttcaagtc gactcggtgt
     2821 catttatccg gacagcctgg tataccctta tacatacaca atgccgggcg atactttctc
     2881 gttcttcaat attgcaggca acagaagata tgaaatcacc aaccacttag gaaacgtgtt
     2941 agctacaatt acagataggc gaatcccaca agaagttggc acacctaacg gcattgcaga
     3001 atactatttg gcagatattg ctagcgttca ggattactat ccgttcggga tggtaacgcc
     3061 gggacgctcc tattccgatg gaagtggcta taggtttgat ttctatgcag gagcaggtag
     3121 atgaagtgta tggaaatggg aatttgaata cagcattgtt ttgggaatat gatttgagac
     3181 ttggcaggag atggaatatt gattttgatt cagaaatcta cgaaagcccc tacctggctt
     3241 ttggaagcaa tccaatagga tttaaagatt tactaggaaa taaaaaggat tggtacaaag
     3301 atgatcaagg aattatgcaa tttgacccta cagttcaaag cagtgcagat ctcaatggag
     3361 agggagagcc caaaggtgta tacgttggaa actattctga agagaaaact gcaaacggta
     3421 tagcctatta tcggcatgac ggcagtattt attatacaaa tcaaactgat ggatacaacc
     3481 gtatttggaa taattcagaa ccaaggaaca gagagcaatc tggtgttatt ttaaacgaag
     3541 gtgtgcttgt tttcccagat tataaaaata attcggacac aggcactcct gatgtttatg
     3601 gttatagttt ttcgaatgga ttaatgtatg atcctgtatc caaggatcaa ggtaaatcaa
     3661 caacacagta tccagttgtt acatttatac attctcattt tgctggcctt gacccggggc
     3721 cgtcgtatta ttcaaaatat ggttatggag atcttggtta tgcagcagag aattttccta
     3781 ataaaacaat tattgtactt gcttgggata acaaagttta tggcttgata tccggtcctc
     3841 cacctgatga ccctacaaaa tgggagtatc aagttataga ttatactcat atttccgtta
     3901 ctaaattatt ggaagggtat gatttttcga cgtttatgaa aaaaattaat aaattttaat
     3961 cgatgaggga ttatttaaaa atactacttg cttctttaca tttactgatg ctttgctcct
     4021 gcactaaaaa cgattcgcag ccagactctg aggtaatgat agtgaagaag aatactattt
     4081 tcgatgataa atttctaagt attttagatc aatacgagga atcgacaaat tgcaaaatat
     4141 gcatcaacat tatttatatt gataaaattg aaccgcataa aactattatc acaataaagt
     4201 caattggggc aaatcttgat tatttgaata acaatccacc attgatgtat ttttaccaca
     4261 aggacattgt ttattttgta ttcacgggga tagaagacta ctttaagcca atcaatgatt
     4321 attccggttt attcaatata caagataatg agaaatgcgc ttctagaact tggcaggtga
     4381 tagatagtat gggtgggtat cgcattctca aacaaatagg ctaccctttt tttccattgc
     4441 caagcaaatt tgcggatgca gattcggcaa atctcgataa ttgaatttta tattaatgaa
     4501 caattaattg aatcttgaac tgctaattaa atcttggtta acttaatcat gcacagtggt
     4561 ggtttataca attttgagat atttaaaagt tactatccgt tcgggatggt aacgccggga
     4621 cgctcctatt ccgctggaag tggctatagg tttgggttta acggcaaaga gagtgacgac
     4681 caattgtatg gagagaataa tggatatgat ttgggattaa gaatatataa cccacgccta
     4741 ggtagatttc tatctttaga ttcaaaatca cgggaatacc cttggcagac accctatgca
     4801 tacttcttta ataatcctgt ggcagtaatt gatgtccttg gtgcaggtgc tcccgatggc
     4861 gagaaggaat taagcgaagg agatggcgct acacctacgc agtatacaat aaaaccaaaa
     4921 gacaattttt ggatactaga gaacaaactt ggtttacctc atggtaatct acaaaagtgg
     4981 aatccaacac ttgatcctaa taatttacaa atcggcacag taataaatac ttcggatcca
     5041 tactattatg acatcgaaaa cgcagtagag ttttctgtat taccatttga tggtggagac
     5101 ttcacaccta gatcttttat acctgcacaa tatgatggaa tgcccaccaa tttatggatg
     5161 atatattctg aatttgttaa aggaacaggg cctgtaaaca gcatttttat tgagaatcca
     5221 ctgattactg gtcaacttgc tagtagtaat ggcgtaaatg aatttcgaaa ctttatgtat
     5281 tccaaattcg atggaagtct ttcaaagatg caggaatacg cctcaaatca taatcgagct
     5341 ttagtcacgg attttgatcc gtatggtagt ttttatcctt gggatgagat tggcaatggt
     5401 gtaatgcaat ttataggtag ttgtagtgtt gatgcatttt tatcctatga caacaaaaca
     5461 ataatatacg tagtaacaga ttctaaaagc ttatactctt tgattctgca ttcaacaaac
     5521 cgattagatt atgaaagaac gaagcagggt gatgcttatt caaatacata ccaaaagtac
     5581 atttggtttg aaacgttcaa ttcccaatat ggcccagaaa acaagtaaaa ttcttagatt
     5641 tcttattgct tttctagtag taagcatact gataggttgc aataagtcaa taaatattga
     5701 tgggaatttc tatgttcaaa aggattcact aagacagaag tatattttga gatataaagg
     5761 agaaaatata tttccaaatg aatttgttaa taaagttgga tataatagtg actggatagt
     5821 tgcaaacact tctagctttc aagaaaacat tacaagctcc aaatattata tcattaacaa
     5881 acaaaattat gctgcacaac ttggtgaaac aaaaaatctt ggtgttatgg gtcccttcga
     5941 ttcgattaac tatgaagaaa ctttgagtga gttgcacttg cagaacgaat gtaaatttat
     6001 aattagtttt tagttattca agaatggcgc taaatgctaa ccaaaattaa attcgagtag
     6061 gaattttaaa ttatattaat gttatttgat aattatttat caactagctt tattaatttt
     6121 aagtgaagat tcaacttacc tagttgcata caaaataaaa taaatatttc tgatatacaa
     6181 tttaatcatg atcttgttgt taaagctctt cgccgaaaat tccttctcac aattcgtaag
     6241 aggtggtcaa gttg
//
LOCUS       DAOQHY010000179         6200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_6895_length_6200_cov_4.695362, whole genome shotgun sequence.
ACCESSION   DAOQHY010000179 DAOQHY010000000
VERSION     DAOQHY010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6895_length_6200_cov_4.695362"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="PLL28_12320"
     CDS             <1..622
                     /locus_tag="PLL28_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70154.1"
                     /translation="PPFDPTQHSVTDNCDPDTVYFVNDILPLIVSNCAKSGCHDGSGE
                     EETNELTSYETIINSDYVDAYNANHSDMIEAVTSGGGEDKMPPSPNQPLTSAQIDLLK
                     TWINQGAHNNECSGGCDTTAVSYSTTIVNTMSNYCNGCHSGGSPSGGIDLTTYAGVAD
                     IASDGRLMGSIQHNAGYVAMPYNAALMPECKIDEIRIWVENGFPND"
     gene            615..1025
                     /locus_tag="PLL28_12325"
     CDS             615..1025
                     /locus_tag="PLL28_12325"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70155.1"
                     /translation="MTKTKQLLAFAAAVCLFTGSFLSSCYYDSMEELGYLGTVCDTTA
                     VSFSTDIQSLLDNSCLTCHSATSADALGGGNNMEGYDNLINCVTPGDPSTSSFYNSIA
                     WVSGTSFMPKAEPQLSSCEINTVMAWINQGALNN"
     gene            1471..1956
                     /locus_tag="PLL28_12330"
     CDS             1471..1956
                     /locus_tag="PLL28_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70156.1"
                     /translation="MNMIITLLKLIVTAMAKRIAIIGLLIECIDCTIRVLHVQTIREL
                     METTALTITGQAIDMHVSARQALLLELMRMQIVAIIQIAMTYTLIVFIPITNGIMSFT
                     NITITAIACIIATSMMMHVVLILSAITKSVSSFIRSGQVWLLAPEPEGDNCDIHSYSL
                     F"
     gene            1993..4395
                     /locus_tag="PLL28_12335"
     CDS             1993..4395
                     /locus_tag="PLL28_12335"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNF70157.1"
                     /translation="MFSGKPASAISRFMQTLWKPIQPFYQRAIGQYIDILAKAYPYPV
                     KVLGMFLKGFAGLSGVLFVLYLLILLGAFGLVPNRGELKAVQNYTASEVYSQDSILLG
                     KFFLENRTNAEFEDLPEDLIHALVSTEDARFYTHDGVDFISFIRVLIMSIILNDDAGG
                     GSTITQQLAKNLFGRKDYGPLTMPVNKFREMIIAGRLEDVYSKDEILTLYFNTVPFGE
                     DCYGIESSALRFFNKAPKDLKTEESAVLVGLLKANTTYNPRLNPDKSLTRRNTVLGLM
                     VRHENHYLTRAQADSLQALPLTINYRRESTAEGLAPYFREYLKSQLEDWAKKNPKEDG
                     STWNIYTDGLKIYTTINGELQAYAEDAMREHMASLQKQFDKSWGKTEPWKNAKTDVVS
                     AARKRSERYQTLKDNGYSDKGIQKRFDDSVKTSIFSWNGPIDTVMTPNDSLKHQLRFM
                     QTGFLAMDPRTGYILAWVGGIDYRFFQYDHVRAKRQVGSTFKPIVYATAIEQGMDPCA
                     YIPNKKVTYESYDNWTPGNSDGEYGGYYTMKGGLTHSVNTVAANLIMQTGVQPVIDEA
                     RKMGVTSKLPAVPSIALGTASISLLEMVTAYSCFANRGLRADPKYIVRIESQKGDTLY
                     EDKAAKKTRALSQETADIMIQIMRNVVNAGTASRLRGTYGLGMDLAGKTGTTQDNTDG
                     WFIGYNSRIVAGAWVGAEDPAIKWKTTALGQGAATALPIFGKFMSKSAKDADTRKYVT
                     GGFTAPADSTLALLDCPMWIPDSLNTDSLGFFQRVIGNIKDYLEKKGEDSIITKYPGA
                     EK"
     gene            4392..4820
                     /locus_tag="PLL28_12340"
     CDS             4392..4820
                     /locus_tag="PLL28_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HNF70158.1"
                     /translation="MSNIWFDPNTRLEDFAHWGKNTLGDHLGMQFTNIGDDFLEATMP
                     VDHRTHQPYGLLHGGASVALAETIGSVASALVINHATHMSLGVEINANHLRSVRSGIV
                     TAKCTPIHIGGTIHVWNILISDSQQRPVCISRLTVMIRKR"
     gene            4832..5617
                     /locus_tag="PLL28_12345"
     CDS             4832..5617
                     /locus_tag="PLL28_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70159.1"
                     /translation="MAAATISSPLNDMFSADLRHAESIRKKIVLNYSIIGSVLFIGLI
                     IEAVTWMSIAILIIALFVLVYTRWYGIPVPVFEQAYVDAVTRHIISPWHGLQIDYQSH
                     LKVSELIAGGFIKEAPDYFSGRNLIHGQAGDTYLRISEVYMRSKDKSIFTQTSGEGQL
                     KALVMVADLSSDEEKQENPRTYVINNDALNTLSNQISADLHSSVFAEVRNGKLYAAII
                     HHAGFAYLNPGITKTVYHMAPIERYEKDVNALISLAINAANVQ"
     gene            complement(5626..5847)
                     /locus_tag="PLL28_12350"
     CDS             complement(5626..5847)
                     /locus_tag="PLL28_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70160.1"
                     /translation="MKKLALLTIATFALGALVSSCEKCTVCTAQSIDGEHVSEYCGNE
                     LDVRDFEDFFIDSLAGITETGYCERGPNN"
     gene            5977..>6200
                     /locus_tag="PLL28_12355"
     CDS             5977..>6200
                     /locus_tag="PLL28_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HNF70161.1"
                     /translation="MILTTTNNIEGKQITDYIGIVTGETIIGANLFRDIFASVRDVVG
                     GRSGAYEEVLREAKDIALREMSEKASSMGA"
BASE COUNT         1897 a         1240 c         1345 g         1718 t
ORIGIN      
        1 tccaccattt gatccgacac aacattccgt gactgataat tgtgatcccg acaccgttta
       61 tttcgtgaat gatattttac cactcatcgt cagcaattgt gcgaaaagtg gttgtcacga
      121 tggcagtggc gaagaagaaa ccaacgagtt aacctcgtat gaaactatta tcaacagcga
      181 ttatgttgat gcttacaatg ccaatcatag cgacatgata gaggcagtaa cttcaggagg
      241 cggtgaagat aaaatgccac caagcccgaa tcaaccatta acttcggccc aaattgattt
      301 actgaaaacc tggatcaacc agggagcaca taacaatgaa tgttccggtg gttgtgacac
      361 aacagcagta agctacagca ccacaattgt aaataccatg tcgaactatt gcaacggttg
      421 tcacagcgga ggaagccctt ccggtggcat tgacctcact acctatgcag gtgttgcgga
      481 tatagcatcc gatggaagat taatgggaag tatacagcat aatgcgggat atgtggctat
      541 gccatacaat gctgccttaa tgcctgaatg taaaatagac gaaataagaa tctgggttga
      601 aaatggattt ccaaatgact aagactaaac aattactggc ctttgcggct gcagtttgcc
      661 tttttacagg cagtttcctc agttcatgtt attacgatag catggaagaa ttaggttacc
      721 tcggaacagt atgcgatact acagcagtat cgttcagcac cgatattcaa tcgctgctcg
      781 acaactcatg tcttacctgc cacagcgcca ccagtgccga tgcattgggt ggaggtaaca
      841 atatggaagg atatgacaat ctgatcaatt gcgtaacgcc cggtgaccca tctacaagtt
      901 cattttataa ttctattgcc tgggtatcag gaacaagttt tatgcccaag gctgaaccac
      961 aattatcttc ttgcgagatc aatacagtta tggcttggat aaaccagggt gcattaaata
     1021 attaaggtat ttaaattttg gtgagtatcg atctcaccgg cgactcagga gcagtgattt
     1081 tattattact gctccttttg ttttgtattg ggcaaagaga ttattataaa aatcaaatct
     1141 tgttttatat ctttgcgaca gcaatgagta agaacattat caattgtaga taaaaaccga
     1201 atccgtaaaa atatttttac ccggaccggt tccgggtatt tttttgccca tacatgaagg
     1261 tgttttacta tttattcata aaaacaaatt atatgcaaca tgttgaattt ccgtccgatg
     1321 atagcattac gctaaaacgt agtggctatg cgtgttgcac ctgatcataa cattcatcaa
     1381 gatcggcccg gcaacactat gtccgggctt ttttgtgtcc ggaaatgatg tatttttttt
     1441 cacgcggcat tccgcacaaa tccgatcatt atgaacatga tcattacctt attaaaatta
     1501 attgttaccg ctatggcaaa aagaattgcc atcatcggtt tgttgattga atgcatagac
     1561 tgcaccatac gtgtattgca tgtacaaacc attcgcgaat taatggaaac aactgcgttg
     1621 accatcaccg gtcaggcaat tgacatgcat gtgagtgctc gtcaggcttt attattggaa
     1681 ttgatgcgta tgcaaattgt cgccatcatt caaattgcaa tgacatatac tttaattgta
     1741 ttcataccaa ttacaaatgg tataatgtct ttcaccaata ttaccataac agcgattgcc
     1801 tgcatcattg ctacaagcat gatgatgcat gttgttttga ttttgtctgc aatcacgaaa
     1861 agcgtcagtt cattcattcg atccggccag gtgtggttgt tggcgccgga accggaggga
     1921 gataactgtg atatacacag ttattccctt ttttgagata tgtacaatac cgcaaagaca
     1981 gtacatttgc gtatgttttc aggcaaaccc gcctctgcca tcagcaggtt catgcaaacc
     2041 ctgtggaagc caatacaacc tttctatcaa agggcaattg gtcagtatat cgatatactt
     2101 gcgaaggcgt atccctaccc tgttaaggtt ttaggtatgt ttctgaaggg atttgcgggt
     2161 ctttcgggtg tattatttgt tttatatcta cttattctcc taggagcctt tggacttgtt
     2221 ccaaatcgtg gagagctcaa ggctgtgcag aattacactg cttctgaagt gtattcacag
     2281 gatagcatat tgctgggtaa attttttctt gaaaatagaa ctaatgcaga attcgaagac
     2341 cttcccgagg atctgataca tgcgttggtt tctaccgagg atgcccgatt ctatactcat
     2401 gacggcgttg attttataag ctttattcgc gtgctcatca tgtccatcat tttaaatgat
     2461 gatgcgggtg gcggtagtac tatcacacaa caacttgcta aaaatctctt tggtagaaaa
     2521 gactacggtc ccttaaccat gcccgtgaat aaattcagag aaatgattat tgcagggcgc
     2581 ctggaagatg tttattcaaa ggatgaaatt cttacactct attttaatac ggttccattt
     2641 ggtgaagatt gctatggtat agagtcttcc gctttacgat tttttaataa agctccgaaa
     2701 gatctcaaaa ctgaagaaag tgcagtactc gttggtttac tgaaagccaa cactacatat
     2761 aacccgcgat taaatccgga taaatccctc accagaagaa atacagtact tggattaatg
     2821 gtgcgccatg aaaatcatta tttgacaaga gcgcaggctg atagtttaca ggcattacca
     2881 ctcaccatta attatcgtcg cgagagcacg gcagaaggtc ttgcacctta tttcagagaa
     2941 tatcttaaat cacaattaga agattgggcg aaaaaaaatc cgaaggaaga tggcagtaca
     3001 tggaatattt atactgatgg attgaaaata tatactacca tcaacggtga attgcaggcc
     3061 tatgctgaag atgctatgcg agagcatatg gcatcacttc aaaaacaatt tgataagagc
     3121 tggggaaaaa cagaaccctg gaaaaatgca aaaaccgatg ttgttagtgc agcgcgcaaa
     3181 cgcagtgaac gctatcagac attaaaagat aacggttaca gtgacaaggg aattcaaaag
     3241 cgatttgacg actccgtaaa aacgagtata ttttcatgga atggccccat agatacagtc
     3301 atgacaccaa atgattcgtt gaagcatcag ttgcgattta tgcaaacagg attcttagcg
     3361 atggatccca gaaccggata tattttggca tgggtaggcg gaatagatta tcgctttttt
     3421 caatatgatc acgtgcgggc aaaacgtcag gttggttcta catttaaacc tattgtttat
     3481 gcaacggcaa ttgaacaagg catggatcct tgtgcgtata ttccaaataa aaaagtcact
     3541 tacgaatcat acgataattg gacaccggga aattctgacg gagaatacgg cggctattat
     3601 accatgaaag gcggactcac gcattcagta aataccgttg ctgcaaattt gattatgcaa
     3661 acaggtgtgc aaccggtaat tgatgaagca agaaaaatgg gtgttaccag taaattacct
     3721 gcagtaccta gtattgcatt aggaactgcc agtatttcat tactggaaat ggttactgcc
     3781 tattcttgtt ttgcaaatcg gggcttgcgt gccgatccga aatatatcgt gcgcattgaa
     3841 tcacaaaaag gcgatacctt gtatgaagat aaagcagcga aaaaaacacg cgcactaagt
     3901 caggaaactg ccgatatcat gatacagatc atgcgcaatg tggtaaatgc aggcactgct
     3961 tcgcgattgc gtggtactta tggattggga atggacctcg caggaaaaac aggcacgact
     4021 caagacaata ccgacggctg gtttatcgga tataactcgc gcattgttgc cggagcatgg
     4081 gtaggcgctg aagaccctgc aattaaatgg aaaactaccg cactcggaca aggtgctgcc
     4141 actgccctac ccatctttgg aaaatttatg tcgaaaagcg cgaaggatgc cgataccaga
     4201 aaatatgtta caggtggatt tacggcacct gcagattcaa cgctggcatt gttagattgt
     4261 ccgatgtgga ttccggattc tttgaatacc gattcacttg gattctttca gcgcgtgatt
     4321 ggaaatatta aagattatct tgagaaaaaa ggtgaagatt ccatcatcac aaaatacccc
     4381 ggcgcagaaa aatgagtaac atctggtttg atccgaatac tcgtcttgaa gatttcgccc
     4441 actgggggaa aaatacactc ggcgatcatt tgggtatgca gttcactaat attggcgatg
     4501 attttctgga ggctaccatg cctgtagatc atcgcacaca tcaaccatac gggttattgc
     4561 acggcggagc atctgtagca ttggccgaaa ccattggcag cgtggcatct gcattggtaa
     4621 ttaatcatgc cacacatatg tcgcttggtg tagaaataaa tgccaatcat ttgcgcagtg
     4681 tcagatcagg aatagttacc gcaaaatgca caccaattca tatcggcggt accattcatg
     4741 tgtggaatat tctcataagc gatagccaac agcgaccggt atgtattagt cgacttacgg
     4801 taatgattcg aaaacgctaa atcatcatat tatggcagca gcaacaattt catctccgtt
     4861 aaacgacatg ttttctgccg accttcgaca tgcagaaagc atcaggaaaa agattgtgct
     4921 caattacagc ataatcggca gtgtattatt tatcggattg atcatagaag cggtgacatg
     4981 gatgtcgatt gccatattaa ttattgcatt atttgtgttg gtatatacaa gatggtatgg
     5041 cattcctgta cccgtgtttg agcaagcgta tgtggatgcc gtaacgcgac atattatttc
     5101 tccctggcat ggattgcaaa ttgattatca aagtcattta aaagtgagtg agcttatagc
     5161 cggtggattt atcaaagaag cgccggatta tttttcagga agaaatctca ttcatggaca
     5221 agccggtgat acctatttac gcatttcgga agtgtatatg cggtcaaaag ataaatcgat
     5281 attcacacaa acatccggcg aagggcaatt aaaggcattg gttatggttg ccgatttgtc
     5341 atcagatgaa gaaaagcagg aaaacccgag aacctatgtg atcaataatg atgcactgaa
     5401 tacattatca aaccaaataa gtgccgattt gcatagcagc gtatttgctg aagtgcgaaa
     5461 tgggaaacta tatgctgcca tcatacatca tgcagggttt gcctatttaa atcccggcat
     5521 tacaaaaacg gtttaccaca tggctccaat agagcgttat gagaaagatg tgaatgcatt
     5581 gatctcactg gcgatcaacg ctgcaaatgt gcaataagca aatgcttaat tattaggtcc
     5641 gcgctcgcaa taacctgttt ccgtgatgcc tgcgaggcta tcaatgaaga aatcttcaaa
     5701 atcgcgcacg tcgagttcgt ttccgcaata ttcactaaca tgctcaccgt caatactttg
     5761 agcggtacaa accgtacatt tttcgcaaga tgacactaaa gcgcccaatg cgaaagttgc
     5821 gattgtgagg agagcgagct ttttcatttt cagcagtttg gatacaaata tacgaataca
     5881 agacaaacga caatatcgga ggccttatta cgtcattgca gatatcaaca tgtatatttg
     5941 taaaacaggc atatgtatca agtaaacaac ccggacatga ttttaacgac cacaaacaac
     6001 attgaaggca aacaaattac agattacatc ggcatagtaa ccggtgaaac gatcatcggt
     6061 gccaatctct tcagagacat cttcgccagt gtgcgcgacg ttgtgggtgg ccgctcaggc
     6121 gcttatgaag aagtgcttcg cgaagcaaaa gatattgcgc ttagggaaat gtccgaaaaa
     6181 gcttcgtcaa tgggcgctca
//
LOCUS       DAOQHY010000180         6150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_6988_length_6150_cov_7.422642, whole genome shotgun sequence.
ACCESSION   DAOQHY010000180 DAOQHY010000000
VERSION     DAOQHY010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6150
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6988_length_6150_cov_7.422642"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..1489
                     /locus_tag="PLL28_12360"
     CDS             188..1489
                     /locus_tag="PLL28_12360"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF70162.1"
                     /translation="MNPLHNRAFQYFLSMRVFITFAFLIQYVVAGWEIYSITKDPFSL
                     GLIGLAEAIPAISIALYAGHLADISNKRDLLIGALVGMLLSSLGLTVFTSAFMREINS
                     DHTTVMLMYLFIFTTGLARGFYGPTATAIIAQIVIPRKKSFPLKHTLDAIDEEEMRDG
                     ESAKIHSTTLVKASTTNSTVWQMSAIIGPALGGFLYGWIGMTGSMIAVLSCIILGIAM
                     LSQVPSTPPQPSQGEPILERLKAGIRFVFDNKIILNALSLDMFSVLFGGAVALLPVFA
                     NDILKCGPQGLGILRAMPSLGAVITISYISWKKIPGKVGTTLLWAVAGFGVTIILFGL
                     SKNFYLSLFLLFLNGAFDSVSVVIRANIIQLLTPDNMRGRVSAVNTMFIGSSNELGAF
                     ESGLAARLLGTVPSVVFGGTITLCIVGFTSLRAKALKAFEY"
     gene            complement(1509..2273)
                     /locus_tag="PLL28_12365"
     CDS             complement(1509..2273)
                     /locus_tag="PLL28_12365"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNF70163.1"
                     /translation="MREWTDFGFITLWVLSFSVLALRYSLFAGLAFYIMQVVVIRKMV
                     SKRLRQEKIDSKYVFIDIGRSCLSFFIFGAMMAATMIAHQHGYTKLYGRWSDQSYWYA
                     FGSFLLLLFIHDTYFYWMHRLLHTKLLYRYIHKHHHRPTPPTPFTSFAFHPLEAIVEF
                     VFIIPAVMILPLHPIVLFAFANVMTAMNVWGHLGFELFPEKYLQGMVGRIMNSTTHHD
                     MHHQYNKCNYGLYFNFWDSIMHTNHKQYRRLQKREK"
     gene            complement(2287..3120)
                     /locus_tag="PLL28_12370"
     CDS             complement(2287..3120)
                     /locus_tag="PLL28_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70164.1"
                     /translation="MKKHLILPIASLTAVMLAFTACDRSEEEILTEPDPTSAKTSAEA
                     TKTFDDVAMMTEEATVEYMDNPDYDTYTFGPCVTVLHDSINTILTFDLGTTGCVGLDG
                     HMRKGKIIVDYNGVPREPGCTLSITLEDFSVDGYSVSGTLTYQAVDYNTSGDLEIAYA
                     VDNGIYTEPDGDVVTFECERTVTWTEGAGSEDLYDDVFTTNGAFTATLNGDAYSGATV
                     NDIVFKSACWADGIYYPVSGTWQLNFPDGINRVIDYGNGDCDKTVVITVGLRDYTYQL
                     P"
     gene            3321..4343
                     /locus_tag="PLL28_12375"
     CDS             3321..4343
                     /locus_tag="PLL28_12375"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNF70165.1"
                     /translation="MMNQKWYQRIWLLHAAFWSGYLMLLTLIFARFLPIDQAFLRNLV
                     NGCAMAPMVYINLFILVPKLLLKKQFRLYTLCLLGLLIIITPLRLWLDDQFTFNTHNP
                     FPQDSIAHIATIIFSSILMLGVTTTLILFQDWYEKSQMAAAYEKLQLEAELKFLKAQI
                     NPHFLFNALNNIYTLSYLDGKAAAPHIMQLSGLMRYMLYESDEHLVSLSKELTYISHY
                     LELQQLKNGKSQNVTWRVEGETGGVLIAPLILIPFFENAFKHGNAMDDQSGYIHGSLI
                     IGREKMIFHLENSIEKTHKRKDEVGGVGLENVRKRLQMLYHEKHSLSVTMAEYTFTIQ
                     LTLMLA"
     gene            4340..5059
                     /locus_tag="PLL28_12380"
     CDS             4340..5059
                     /locus_tag="PLL28_12380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNF70166.1"
                     /translation="MSYRCLIVDDESLALRLMEDHVSKVPGLEIVGKCRHALEALQVL
                     QREEIDILFLDIQMPDLTGVELIKILKHKPAIILTTAYAEYALEGYQLEVVDYLLKPI
                     ALDRFLQAVRKAQEWVDLRKQAPSVPQTTKQDHFFVKSDYKQVRINYADVTHVEGLRE
                     YVSIYAGGKRIVTLETMKNMEQLLPSDLFMRVHKSYIVNTSRIKSINGNQIELGEIRI
                     PLGKVFRAQVLGRLGIISSEG"
     gene            complement(5317..5781)
                     /locus_tag="PLL28_12385"
     CDS             complement(5317..5781)
                     /locus_tag="PLL28_12385"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNF70167.1"
                     /translation="MKELDHNKLKQINNSALLALCILLILGGGLVWEVFTSSSKIFSY
                     EEQLVLGECGVTNNFVYETGNSPEGELLFQVKCQRCHLVDRKMTGPALMGVRNRWADS
                     TDLYSWIKNSQKFLETGDKYAIDLYKAYGTIMPPFPELSDEQIAELLIYVSP"
BASE COUNT         1889 a         1224 c         1231 g         1806 t
ORIGIN      
        1 ttccttcttt aaaactaaca ttctagatga aaaagacctg tttaaggtct aaaggcctct
       61 gcgtgaaaaa ttgatgagtg atgactcgtg agtgtggtat gtgattcacg agtaaacact
      121 actcattgcc aaacggccaa aacaaatcca aagtcacaat attcttttcg atgtgtaatt
      181 ttaccctgtg aatccactcc acaaccgggc atttcaatat ttcctcagca tgcgtgtgtt
      241 tatcacattc gcatttctta ttcaatatgt ggttgcagga tgggaaatat attccatcac
      301 caaagatcct ttcagtttag gtttgattgg tttggctgag gccattcctg cgataagtat
      361 agcgctgtac gccggccatc tggctgatat cagcaacaag cgcgatttgt tgataggcgc
      421 gcttgtaggt atgttgctat catcattggg actaaccgtt tttacctcgg catttatgcg
      481 tgaaatcaat agtgaccata ctacggtcat gttgatgtat ttattcattt tcaccaccgg
      541 acttgcacgt ggattttatg ggccgacggc tactgcaata attgcacaga ttgttattcc
      601 tcgcaaaaaa tccttccccc taaaacatac gcttgacgca attgacgaag aagagatgcg
      661 agatggtgaa tcggcgaaaa ttcacagtac cacacttgta aaagcatcta caacaaattc
      721 tacggtctgg caaatgagcg ccatcatagg accggcactt ggtgggtttt tatatggttg
      781 gattggcatg acgggttcta tgatcgcagt attatcgtgc atcatattag gcattgcaat
      841 gttgtcgcaa gtaccatcaa caccaccaca gccatctcaa ggcgaaccta ttttggagag
      901 attaaaagca ggtattcgtt ttgtattcga caacaaaata attctaaatg ccctgagttt
      961 ggatatgttt tccgtgcttt tcggtggggc agtagcccta ttaccggtgt ttgccaacga
     1021 tattttaaaa tgtggtccgc aaggtcttgg aattttgcgt gctatgccat cattaggtgc
     1081 ggttataact ataagttata tctcatggaa aaaaattcct ggtaaggtag gcacaacatt
     1141 gttgtgggca gttgccggct ttggtgtaac tattattttg tttggactta gtaagaactt
     1201 ttacttgtca ttgtttttat tatttctaaa tggcgctttc gacagtgtaa gtgtagtgat
     1261 acgggcaaat atcattcagc tcctcacacc tgataatatg cgcggacgcg ttagtgcggt
     1321 aaacaccatg ttcatcggca gcagtaacga gcttggtgcc ttcgaaagcg gacttgccgc
     1381 caggttgctt ggaacagtgc cctcagtagt ttttggagga acaattacct tgtgcattgt
     1441 aggatttaca tcattgcgcg cgaaagcatt gaaggctttc gaatactgaa ataaataatt
     1501 ctcttaaatt atttttccct tttctgtaaa cggcgatatt gtttgtggtt agtatgcata
     1561 atgctgtccc agaaattaaa atataagcca taattacatt tattatactg atgatgcata
     1621 tcgtgatgcg tggttgaatt cattattcta cctaccatac cttgcaaata tttttcggga
     1681 aataattcaa atcccaaatg cccccaaaca ttcatagctg tcatcacatt tgcaaatgca
     1741 aacaagacaa taggatgtaa gggcaaaatc atgacagcag gaataataaa aacaaattcc
     1801 acaatagctt caagcggatg aaatgcaaag ctcgtaaaag gcgttggcgg tgtagggcga
     1861 tgatgatgtt tatggatata tcgatataac aatttcgtat gcagtaatct atgcatccaa
     1921 tagaaatacg tgtcgtgaat aaataacaat agaaggaatg agccgaatgc ataccaataa
     1981 gattgatcgc tccatctgcc atacaatttt gtataaccat gttgatgggc tatcatagtc
     2041 gcagccatca tagcaccgaa aatgaaaaaa ctgaggcacg atcttccaat atcaatgaac
     2101 acgtatttgc tatcaatttt ctcttgcctt agtcgtttag ataccatttt gcgaatcaca
     2161 acaacctgca taatataaaa agccagccct gcgaataggg aatatcgcag agccagcaca
     2221 ctaaacgaca gcacccagag ggtaatgaat ccgaaatctg tccactcacg catggaatta
     2281 tttcaattag ggtagctgat aggtataatc acgcaatccg acagtaatca cgacagtttt
     2341 atcgcaatcg ccgttgccat aatcaataac gcgatttatg ccatcgggaa agtttaattg
     2401 ccatgtgcct gaaacgggat aataaatgcc atcagcccaa catgcagatt taaatactat
     2461 atcattaacc gtagcgccac tgtatgcatc accatttaag gtagcagtaa atgctccatt
     2521 agtggtgaat acatcatcat acaaatcttc acttccggca ccttcagtcc acgtaacagt
     2581 tctttcgcat tcaaaggtca ccacatcacc atcaggctct gtataaattc cattatccac
     2641 agcataagcg atttcaagat caccggaggt attataatca acagcttgat atgtcaacgt
     2701 gccgcttacg ctgtatccat caacagaaaa atcttcaagc gtgattgaca aggtacatcc
     2761 cggttcacgc ggcacgccgt tgtagtcgac aataatttta ccttttcgca tatgaccatc
     2821 gagtcccaca caacctgttg ttcccaaatc gaaagtcaat attgtattga tagagtcgtg
     2881 caatactgtt acgcagggac cgaaagtata agtatcgtaa tcagggttgt ccatatactc
     2941 gacggtggct tcttcggtca tcatagcaac atcatcaaaa gtctttgttg cctctgcaga
     3001 agtttttgca gatgtaggat ccggttcagt aaggatttct tcctccgaac gatcacaggc
     3061 cgtaaatgcc agcatcacag cagtgagtga ggcaataggt aagatcaagt gtttcttcat
     3121 atattgttgt tttgatgaag caaaagtgca tcgataattt atgtgcaaaa aacttaatca
     3181 acaggtattc aataaatcac aatgacagta aatatttcat ctacaagcgt tatttcgatg
     3241 cttgattttt cgttcgtaga aataaatgtg acgttggtag ataaactgcc aaataaatgc
     3301 ccaatggcac gtaagtttgt atgatgaatc aaaagtggta tcaacgtata tggctgctgc
     3361 atgctgcctt ttggagtggt tatctgatgc tgcttacgtt gatatttgcc agattccttc
     3421 ccatagatca ggcattcctg cgcaatctgg tgaatggctg cgcaatggct cccatggtat
     3481 acattaacct gtttattttg gtgccaaagc tgctgctcaa aaaacagttt aggctttaca
     3541 cgttatgctt gttgggctta cttatcatca tcacacctct gcgcttgtgg cttgatgatc
     3601 aatttacgtt caacacccat aatccatttc cgcaagatag cattgcgcat atagcaacaa
     3661 tcattttttc atccattctt atgttgggag ttaccaccac cttaatattg tttcaagact
     3721 ggtatgaaaa atcgcaaatg gctgctgcat atgaaaaact gcaattggag gctgaattga
     3781 aatttttaaa ggcgcagata aatccgcact tcttatttaa tgccttaaat aatatatata
     3841 ctcttagtta tcttgacggc aaagccgcgg ctccacatat tatgcaattg agtgggttga
     3901 tgcgctatat gctttatgaa agtgatgaac accttgtttc cttatcgaag gagcttacat
     3961 atatcagtca ttatcttgaa ttacagcaat taaagaatgg caaatcgcaa aatgtaacat
     4021 ggcgtgttga aggagaaaca ggaggcgtat taattgcgcc actgatatta attccatttt
     4081 tcgaaaacgc attcaaacat ggaaatgcaa tggatgatca atcgggatat attcatggaa
     4141 gcttgatcat tggcagagaa aaaatgatat tccatctcga aaacagcata gaaaaaacac
     4201 ataagcggaa agatgaagtt ggcggagtgg gattggaaaa tgtgcgcaag cgattgcaaa
     4261 tgctatatca tgaaaaacac agcctgagtg taaccatggc cgaatacact tttactattc
     4321 aattaacgtt aatgttagca tgagttacag atgtttgata gttgatgatg aatcgcttgc
     4381 attgcggttg atggaagatc acgtgtcgaa ggtgcccgga ttagaaattg ttggcaaatg
     4441 cagacatgcg cttgaagctt tgcaggtatt acaacgcgaa gaaattgata ttttatttct
     4501 cgatatccag atgcctgacc tcaccggtgt ggaattaatt aaaatcctca agcacaaacc
     4561 ggcaatcatt ttaaccacag catatgccga atatgcgctg gagggatatc aattagaggt
     4621 tgtagattat ttattaaaac ccattgcatt ggatcgtttc cttcaggctg ttcgaaaagc
     4681 acaagaatgg gtagatctgc gaaaacaagc tccatcagta ccacaaacaa ccaagcagga
     4741 tcattttttt gtaaaatcag attacaagca agtgcgcatc aattatgcag atgtaacgca
     4801 tgttgaggga ctacgagaat atgtaagtat ttatgccgga gggaaacgca tagttacatt
     4861 agaaaccatg aaaaacatgg agcaattatt accctcggat ttgtttatgc gtgtccacaa
     4921 atcttatatt gtaaatacat cgcgcataaa atcaataaat ggcaatcaga tagaattggg
     4981 tgaaataaga attccactcg gcaaagtatt tcgtgcacaa gttttggggc ggttgggtat
     5041 tatatcgtct gaaggttgag gtaaatggaa gggggtttga agagttgaag attcgaagag
     5101 tcgaagattc gaaggtttga agagtcgaag gtttgaagag ttgaagattc gaaggtttaa
     5161 ttcacgagtc gctattcacc attaacacat attcaatcga agagttggct gggtaatgca
     5221 aaataaccca agagaaaaag ttcaattatc tatattgatt attcctcatt gctataactt
     5281 aaatacatct aacacaggaa gaattggtca aagtgctcat gggctcacat agatgagcaa
     5341 ctcagctatt tgctcatcag agagttcggg aaatgggggc atgatagtac catacgcttt
     5401 atataaatca atggcatatt tatcgcctgt ttccagaaac ttttgactgt tttttatcca
     5461 tgaatacaaa tcagtagaat cagcccatct gttgcgcaca cccattaatg ccggtccggt
     5521 cattttcctg tcaactaaat ggcaacgctg gcatttgact tgaaacaata gttctccttc
     5581 cggcgaattt cccgtctcgt aaacaaaatt atttgtaacg ccacattctc ctaaaaccaa
     5641 ttgctcctca tatgaaaata ttttactgga tgatgtgaat acctcccata caagtccacc
     5701 ccccaggatc aataaaatac ataatgccag caaagcagaa ttgttgattt gtttcaattt
     5761 attatgatcc aattctttca taaaatgacg aggacttatt caaatataga tatataaagc
     5821 aagataaaat aatccaacaa aagcaattta cagagtatta cataattgtg aatggttata
     5881 gcagatgtaa tatggattat gcaaaaataa ttgcactccc tattaacgcg ccagcgttaa
     5941 aagcacccac acttgctaat ttgctaattc tcacacttgc taattaacct cgcttgctaa
     6001 ccctctttca gtatctagaa tcaagtatct agtatctaga cttttgcgcc ttagcctctt
     6061 cgctgtcttt gcgcgaaacc cgcctcaccc ccggcccctc tccatctcct atcgtcgata
     6121 gagagggagc tgattgcgct tcggaattaa
//
LOCUS       DAOQHY010000181         6142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_7003_length_6142_cov_6.608510, whole genome shotgun sequence.
ACCESSION   DAOQHY010000181 DAOQHY010000000
VERSION     DAOQHY010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6142
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7003_length_6142_cov_6.608510"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /gene="dnaN"
                     /locus_tag="PLL28_12390"
     CDS             complement(<1..638)
                     /gene="dnaN"
                     /locus_tag="PLL28_12390"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNF70168.1"
                     /translation="MKFIVSTTALLRNLQTVNGVVSPNTVLPILEDFLFVVEKNKLTI
                     SGTDLETSITTWMDIESKSEGKVAIPARILVDYLKTLPEQPLTFSIDEESCQVEITTD
                     RGKYKLNGENGDDFPRVSSDDKLSEIALPSGVLQKVINKTAFATSNDELRPPMTGVLF
                     QLNKDGVNFVATDAHKLVRYRRTDIKADKASSLIMPKKALNLLKSNLPGEQT"
     gene            764..2338
                     /locus_tag="PLL28_12395"
     CDS             764..2338
                     /locus_tag="PLL28_12395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70169.1"
                     /translation="MLRKTLGFLTLLIAVNRLTLSAQDATPPFTISLEESTYGDWPGL
                     QSYVAGTWDGKWILLAGRTGGLHAFLPPNPFMPMEANKEVHVLDPLTGDHWSSSVTIL
                     PEDLSNQMQSTNPQAFQRGNTLYVIGGYGHDETAMEMRTFPALTAIDLDILGNAVING
                     TDITPAFRTIQDTIFEVTGGEIGMLGDTVYLFGGHSFTGVYSKPAGPQFTQVYTNRLT
                     KFRIVDDGTDLSIADLEFITDTTAFHRRDLNFEPLMLPGEEHALIALSGVFQYEADWP
                     WHEPVVVTSGGYYVDEALYQKMNNYTCPVMSIYDADSEKSYLTLFGGFSEYWFDADDS
                     ILKQDLNIPFVHDISTVIRNTDGTMTQYIQPISLDGLLGSNAEFLINPEIPQYDDRII
                     KLTEIDGDVLAGYIFGGIDAEIPNFTPSIASNRLFKVWLHYERETPVVDLTQNGIKVY
                     PNPVMDELTIQNLAQYGITGISIADMLGNTLINQSSAIPSGYRKTISLEKLPAGVYMV
                     KVSSANGFTMQQVIKQ"
     gene            2438..3724
                     /locus_tag="PLL28_12400"
     CDS             2438..3724
                     /locus_tag="PLL28_12400"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70170.1"
                     /translation="MKKITLVSLWLVCFISLKAQFPVISPAVETALTLTFPISVSNSG
                     NVAWNPSANLYYSCRIGNAVYPLITWNALGVQMDYDTTGQDSRGMWWNASLNQLERNC
                     FSSVGWAKINLDANSYAENTFSILFSGMLQPNAQSVGTYDPDANQVIFYNAGNCYKYD
                     RTTGLLVSTLALTGATLTTITANGIVYTGVTGAEIGIYDYSAKKVLLFNKTTGVFSAS
                     STLPATAPGPTTYGIGYANNLFWVYKTSPESKWYSYRIFDIPLPVYAIALAGNQIANA
                     NYLHWTVDADEPVVSFDLQHSSDGKTFKSIASIPGSSDGSEVYEYMHQFPAPGANYYQ
                     VISTDQQGEKHTSDIVCITSESLSSVLKVFPNPADEVISIGGLAGGMSYTIYDESGRQ
                     IASGNSNPDGTISLTDLPSGNYFLYLASTPIRFIKQ"
     gene            3779..4159
                     /locus_tag="PLL28_12405"
     CDS             3779..4159
                     /locus_tag="PLL28_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70171.1"
                     /translation="MRLTYTLVIGLILASCAHSETETRTPVQIKEDLRRAELHSPTMY
                     LRVSGELKEDSVFCLINNTAKEADYPNAIIAVNYFNDSRQLIKSELINVGNQILKGTT
                     INFNSKVDPPDEYASVSFSVREVK"
     gene            complement(4227..5270)
                     /locus_tag="PLL28_12410"
     CDS             complement(4227..5270)
                     /locus_tag="PLL28_12410"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNF70172.1"
                     /translation="MNRNWLYFGILVILCVVAYFTLIRDNSSSYSKKDAAFAIADTND
                     IYRIVLTNMKGDTIDLKRNQDDWLVNDLYAPRPDAMHNLLRTMNQMAVKVPVSKSMHN
                     NVIRNISGRHTHVQIFDAKNKLIRGYYIGDNTDQLNGTFMLMEEADMPFVVNIPGFQG
                     YISSVFFTDITDWRSKEIFAYSTAEIQQIDVTYPAVKDSTFSLVKQQNGSFLIVANKQ
                     ENKPINQEIVQFYLKQFARLNAEYYINEPDKRDSLLGGQPACLMSVTDIQKRTTTLKI
                     YYRPVTVRSKAQFTYDDKPLQFDPDKFYGIFHGDKDLAIIQNFVFGKVLVGPGFFYRQ
                     RPGGSNMLSEQTK"
     gene            complement(5280..>6142)
                     /locus_tag="PLL28_12415"
     CDS             complement(5280..>6142)
                     /locus_tag="PLL28_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70173.1"
                     /translation="LYLGNGQFMAIDRELNIIDQMVQYGARVNSNIIQDKQCTHINVP
                     VAKGDNFIVRPWPYNPILNNYNTDHPVTKNVDAIEGKFVSTVDTLAVPGITKTILLKT
                     SGQSRYLNAPARVNFNIVDPKFAPTDQQYNKPELPVAVLLEGNFHSAFRSIRPTAKTL
                     HDRGYGYTDTTYMAEGKPAKQIVIGDGDLIKNFVDKEGKPDMLGVNYTERYIFGNKDF
                     AMNCIEYLCDQNGLIETRAKEVKLRPLDDQRVEKDRTQWQVVNMIAPIFLLYIFSGIY
                     FFIRYRKYAA"
BASE COUNT         1748 a         1276 c         1261 g         1857 t
ORIGIN      
        1 tgtgtttgtt cacccggaag atttgacttc aataagttca aagctttttt aggcatgata
       61 agcgatgatg ctttatcggc cttaatatct gttctgcggt agcgcaccag tttgtgtgca
      121 tcggtagcaa caaagtttac accatcttta tttaattgaa agagcacacc cgtcattggc
      181 ggacgcaatt catcgttgct ggtagcaaat gcagttttat tgattacctt ttgcaataca
      241 cctgatggca atgcaatttc cgataattta tcatcgcttg aaacgcgtgg aaaatcatcg
      301 ccattttcgc catttaattt gtatttaccg cgatcggtag taatctctac ctgacaagat
      361 tcttcatcta tgctaaatgt caatggttgc tcgggaagtg tcttcagata atcaacaaga
      421 atacgcgccg gtatggcaac ttttccttcc gattttgact caatgtccat ccaagtggtt
      481 atagatgttt caaggtcagt tcccgaaatg gtcagtttgt tcttttcaac aacaaaaaga
      541 aaatcttcca gtataggtaa aacagtgttg gggcttacca caccgttaac ggtttgcaga
      601 ttgcgcaata gggctgttgt gcttacaata aacttcatgt actacttttt gaatacaggc
      661 aaaggtaaac aaacagtaag aatgtttttc aacaaggggt ggatagcgct gtactgttct
      721 aaaagtcacc aaatgcaatg cccacgcacc ttatctttgt tccatgttgc gcaaaaccct
      781 tggctttctt acactgttga tagcagttaa ccgcttaacc ctttcggcac aggatgctac
      841 tcctcctttt accatttctc ttgaggagtc aacttatggc gattggccgg gattgcaatc
      901 ctatgttgca ggaacctggg atgggaaatg gatattattg gcaggtagaa cagggggttt
      961 gcatgccttt ttacccccaa atccgtttat gcctatggag gccaataagg aggtgcatgt
     1021 gctggatccg cttaccggag atcattggag cagcagtgta accattcttc ccgaagacct
     1081 ctccaatcag atgcaaagca ccaatccaca agcttttcag cgtggaaaca ctttatatgt
     1141 cataggaggg tatggtcacg acgaaacagc catggaaatg cgcactttcc ctgcacttac
     1201 ggctatcgac ctcgatatat tagggaatgc ggttataaat ggtactgaca tcacccccgc
     1261 atttagaacc atacaggata ccatatttga ggtgacaggt ggtgaaatcg gcatgttggg
     1321 cgataccgtg tatttatttg gaggtcactc atttaccgga gtgtattcaa aacctgcggg
     1381 acctcagttt acacaggtct ataccaaccg ccttaccaaa ttccgcatag tagatgatgg
     1441 cactgactta tccattgccg accttgaatt cataacagat acaacggcat ttcaccgtcg
     1501 cgacctgaat ttcgagccat tgatgcttcc cggagaagag catgcgctaa ttgcgttgag
     1561 cggtgttttc caatatgaag ccgattggcc atggcatgaa cctgttgtag taacttccgg
     1621 cggttattat gttgatgagg ccttgtatca gaaaatgaat aactatacct gcccggtaat
     1681 gtctatttat gatgcggatt cagaaaagtc ttatttaacc ttgttcggcg gattcagtga
     1741 atattggttt gatgctgatg attctattct aaaacaggat ttgaatattc ctttcgtgca
     1801 cgatatttct accgttataa gaaataccga tggcaccatg acacaatata ttcaaccgat
     1861 ctctttggat ggtttgttgg gatctaatgc agaattttta atcaatcccg aaattccgca
     1921 atacgatgat cgcatcatca aacttaccga gattgatggt gatgttttag caggatatat
     1981 tttcgggggg atagatgcag agataccgaa tttcacaccc tctatcgcct cgaatagact
     2041 gttcaaagta tggctgcact acgaaagaga aacacctgtt gttgatttaa cgcaaaatgg
     2101 tatcaaagtg taccccaatc cggtgatgga tgagcttact atacaaaacc ttgcacagta
     2161 tggcatcaca ggcatttcta ttgcagatat gctgggcaat acactcatta accaatcatc
     2221 cgctataccg tccggttaca gaaaaactat ttctctggaa aaacttccgg ccggtgttta
     2281 catggtaaaa gtgtcgtctg caaacggttt caccatgcaa caggtaatca aacaataggg
     2341 gagttactaa aatcacccga ccggggtatt tcagaaaggc acggaaacag gtccttttgt
     2401 gacatcaaaa attatgtcac aaaatcactt gattccaatg aaaaaaatta cactcgtatc
     2461 gctttggcta gtatgtttta tttcactcaa agcacagttt cctgttattt cacctgctgt
     2521 tgaaaccgct ttaacgctaa cctttcccat ttcagtaagc aactccggaa atgtagcctg
     2581 gaatccatcg gctaatcttt attattcgtg tagaataggc aatgccgttt atcctttaat
     2641 aacctggaat gcattaggtg tgcaaatgga ttatgacaca accggacaag acagcagagg
     2701 catgtggtgg aatgcaagtt tgaatcaact ggaaagaaat tgtttttcgt ctgttggttg
     2761 ggcgaagata aatttggatg cgaatagtta tgcagaaaat acattcagca tattattcag
     2821 tggtatgtta caaccgaatg cacaaagtgt cggaacatat gatcctgatg ccaatcaagt
     2881 gattttttac aatgccggta actgttataa gtacgatcgc acaaccggat tgctagtatc
     2941 taccctggca ttaaccggtg ccacgctaac caccattaca gccaatggta ttgtttatac
     3001 tggtgttacg ggagctgaaa ttggaatata tgactattca gcaaaaaaag tattgttgtt
     3061 taataaaaca accggtgtgt tttccgccag ttcaacgcta cctgcaaccg cgcctgggcc
     3121 cacaacatac ggtatcggat atgccaataa tcttttttgg gtgtataaaa catcccctga
     3181 aagtaaatgg tattcttata gaatttttga tatcccctta cctgtatatg ctattgcatt
     3241 agctggaaat caaattgcca atgctaacta tttgcattgg acagttgatg cagatgagcc
     3301 tgttgtttca tttgatctgc aacattcgtc tgatggaaaa acatttaaga gtattgcttc
     3361 cattcccggt agctctgatg gctctgaagt ttatgaatat atgcatcaat ttccggctcc
     3421 gggagcaaat tattatcagg taatctcaac cgatcagcaa ggcgaaaagc atacctctga
     3481 tatcgtgtgt attaccagcg aatcgctgag cagtgtgttg aaggttttcc ctaatccggc
     3541 agatgaagtt atcagtattg gtggattggc aggtggtatg tcttatacca tttatgacga
     3601 gtccggaaga caaatagcca gcggcaacag caatccggac ggcactatct cacttacaga
     3661 tcttccttcc ggtaattact tcctatacct cgccagcacg cctattcgct tcataaaaca
     3721 gtaaaaaaaa tgggtatttg tgacaaatgt gttaatttag tattcctaaa cacacacaat
     3781 gagattaacc tatacccttg tcataggtct gatcttggct tcttgcgccc atagcgaaac
     3841 ggaaacgcgc acaccagtcc agatcaaaga agacctacgc agagctgaac tgcattcgcc
     3901 aaccatgtat ttacgtgtga gtggtgaact gaaagaagat tcagtatttt gcctgatcaa
     3961 caacactgca aaagaagcgg attatcctaa tgccatcatt gctgtaaatt acttcaatga
     4021 ctcaaggcaa ttgataaaaa gcgaattgat aaatgttggc aatcagatac tgaaaggcac
     4081 cacaatcaac ttcaattcaa aggttgaccc tccggatgaa tatgcttcgg tgtcattttc
     4141 tgtaagagaa gtgaaataat aatttccaag gaagcaatta cttttttatt tcctttttgc
     4201 tttcagcgtg aaccgaggaa gttgtattat ttcgtttgtt cactaagcat attgctgcca
     4261 cccgggcgtt gtcgatagaa aaatccgggg cctaccagca ccttaccgaa tacgaaattt
     4321 tggatgatag caaggtcctt atcgccatga aaaatgccgt agaatttatc cggatcaaat
     4381 tgaagcggct tatcatcata agtaaattga gccttgcttc gaaccgtaac cggacgatag
     4441 taaattttaa gcgtggttgt gcgcttttga atatctgtta cagacattaa acatgccggt
     4501 tgcccaccta ataatgagtc gcgcttgtcg ggttcgttga tgtaatattc ggcattcaat
     4561 ctggcaaact gctttaaata aaattgcaca atctcctgat taattggttt gttttcttgc
     4621 ttgttggcaa caatgagaaa actgccgttt tgttgtttca ccaatgaaaa tgttgaatcc
     4681 tttactgccg gataggtgac atcaatttgt tgaatttcag ctgtgctgta agcaaatatt
     4741 tctttgctgc gccaatcggt gatgtcggta aaaaatacgc ttgaaatata tccttgaaaa
     4801 cccggaatat ttaccacaaa tggcatgtcg gcttcttcca tgagcatgaa agtgccattt
     4861 aactggtcgg tattatcgcc gatataatat cctctgatca atttattttt tgcatcgaaa
     4921 atctgaacat gcgtatgcct gccgctaata tttctgataa cattattgtg catggatttc
     4981 gataccggaa ccttcacggc catttgattc atggtgcgca ataaattgtg catggcatca
     5041 ggccttggag cataaagatc gtttaccagc caatcatctt gatttcgctt cagatctatc
     5101 gtgtcgcctt tcatattggt aagcacgatg cggtaaatat catttgtgtc ggcaatagca
     5161 aatgctgcat cctttttact gtacgaagaa gagttgtctc ttattaatgt aaaataggcg
     5221 acaacacata aaataaccag gattccaaaa tatagccaat tgcggttcat tgatattatt
     5281 tatgctgcgt atttgcgata tcgtataaag aaataaatgc cactgaaaat gtataataaa
     5341 aatattggag cgatcatatt caccacttgc cattgcgtgc gatctttttc cacgcgttgg
     5401 tcatccagcg ggcgcaattt cacctctttg gcgcgcgttt caatcaatcc gttctgatcg
     5461 cataaatatt caatgcaatt catagcgaaa tccttgttgc cgaaaatata gcgctccgta
     5521 taattcacac caagcatgtc gggtttacct tccttgtcta caaaattttt aataagatca
     5581 ccatcgccaa ttacaatctg ctttgcgggt tttccttctg ccatgtaagt ggtgtcggta
     5641 tagccgtaac ctctgtcatg taatgttttt gcagttggac gaatgctcct gaatgcggaa
     5701 tgaaaattgc cttccaacag cacagcaacc ggtaattccg gtttattgta ttgctgatcg
     5761 gtaggcgcaa atttaggatc tacaatattg aaattcaccc tggcaggcgc atttaaatag
     5821 cgactctgtc cgcttgtttt taagagtatc gtttttgtaa ttcccggaac tgcaagcgtg
     5881 tcaacggtgc tcacaaattt accttctatg gcgtcgacat tttttgttac gggatggtcg
     5941 gtattgtaat tgtttaatat aggattatag ggccatggtc gtacgataaa attatcgcct
     6001 ttcgcaacag gcacattgat atgcgtgcat tgtttgtcct gaataatatt actgtttacc
     6061 cgggcaccat attgcaccat ctggtcaata atattgagtt ctctgtcaat agccataaat
     6121 tgaccattgc cgagatataa ac
//
LOCUS       DAOQHY010000182         6140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_7009_length_6140_cov_4.617420, whole genome shotgun sequence.
ACCESSION   DAOQHY010000182 DAOQHY010000000
VERSION     DAOQHY010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7009_length_6140_cov_4.617420"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3055)
                     /locus_tag="PLL28_12420"
     CDS             complement(<1..3055)
                     /locus_tag="PLL28_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNF70174.1"
                     /translation="MNISSVSINRPVLATVISILILLFGIIGYSFLGVREFPSVDPPI
                     ITVTTNYTGANADIIESQITEVLEEQINGIAGISSLSSTSSDGRSTITVEFTLDVDLE
                     AAANDVRDRVSQAARNLPPDCDPPIVAKSSADSNPIVSMTIQSDQRSLLELSEIANNV
                     FKERLQTIPGVSEVRVWGEKKYSMKLLLDPVKLAGYHLTPSDVRDALNRENVELPTGR
                     IEGYTTELSIRTFGRMTTEEEFNDLIILEQDGQVIKLQDVGQAKLMPENERTVLRGNG
                     GIPQVAVAITPQPGSNYIEIADEYYRRFDQIKKELPDDLQYKIALDTTTGIRKAIDEV
                     KETLLIAFGLVVLIIFLFLRDWRTTLIPVVAVPISLIGAFFIMYVSGFSINVLTLLGI
                     VLATGLVVDDAIVVLENIYAKVERGVKPLQAAHEGSKEITFAIISTTITLVVVFLPIV
                     FLEGLTGRLFREFGITVAGSVLISALVSLTLTPMMSARILKHRERHNWLYRTTEPFFE
                     GLNRVYISALEKLMQRRAMVIVLLMGGSIFLGRYCLLLLPEEVAPMEDKGRFVINSTA
                     PEGTSFEKMDAFQQELINIVDTLPEKENIIAVTAPSFGAGIAANSGFIRVSLVEPEER
                     TRSQQEIADEIAAVVKKYNFARTFISQEQTISSDRRAGLPVQYVIQAPDFESLREVIP
                     QFMEKASQDPTFQVVDINLKFNKPELTVEIDRDRARALGVTVRDIAETLQLYFSGQRF
                     GFFIFNNKQYQVIGQATRDNRDEPLDLSKAYVKNDKGELIQMDNLVHLTDQSSPPSLY
                     RYNRYVSATIMANPAPGYTIGDGIDAMDAVAKDVLTEDFSTALSGPSKEFAESSNSLQ
                     FALIMALVLVYLILAAQFESFLDPLIVMFTVPLAFAGAFGSLYLFDQTINIFSQIGII
                     VLIGIVTKNGILIVEFANQRKEAGLDKFAAAMEAARQRFRPILMTSLATVLGALPIAL
                     ALGASAKSRVSMGIAIIGGLLFSLMLTLIVIPLVYTIVSSTKKHTP"
     gene            complement(3060..4115)
                     /locus_tag="PLL28_12425"
     CDS             complement(3060..4115)
                     /locus_tag="PLL28_12425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNF70175.1"
                     /translation="MSKRFLFLISAVALLAACSKSKESSQSAAPAGPPPAQPVKAIIA
                     TTSSLDNHIEATGTILANEEVEIRSETSGRVIKLYFKEGDAVNSGSLLLTIDDRELQA
                     QLTKTNLSIDLATEDEQRKRKLLDAGGISKEEYDASRNYLLKLEADKQLLETQIAKTK
                     VTAPFSGTIGLRYISEGGYVSPATLIASLQQTNMVKVEFSVPEKYASSIKNNATVNFT
                     VEGDDHTFEAKVYAREPKIDPQTRSIKVRAIALNAEQRLIPGAFAKLTINLGTIPEAI
                     EVPSEAIIPAITGQTLYVVRAGTAKVQPIKTGIRTATTTQITEGIVPGDTIITTGLLT
                     VRPGAAVKPIIVENKKD"
     gene            complement(4145..5023)
                     /locus_tag="PLL28_12430"
     CDS             complement(4145..5023)
                     /locus_tag="PLL28_12430"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HNF70176.1"
                     /translation="MNKVNTIIIGAGRSGTTSVFAWMEHHPDIQFSITKEVHYFSLID
                     LYQRGEKYFHSLFEGESKKIIATADTYLLMDHDAPARIKAYNPDMHIIVMLRDPIDRA
                     YSNYQYSVQFGHERSDISFADTITLEQERLLSNDIAAKNNLCHCYGSLYARHLSVWTL
                     SFPKEQIFIGTLDQLKSDADALYKRLSTYLGIQYHAFSQQGEAFNTSTGVKSKWLQQL
                     LLNREHPLRKTLTFFIRPFRNLIIKSGIIDKIYKLNKTQVELPKLTIEERQIVTPYFE
                     QDLKQLEADWHVTFQR"
     gene            complement(5020..6006)
                     /locus_tag="PLL28_12435"
     CDS             complement(5020..6006)
                     /locus_tag="PLL28_12435"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNF70177.1"
                     /translation="MKSIWFSAFRKRYERRALKDFIKASGQQIIFPFYHTVSNDDLPH
                     IKHLYRWRNVQEFESDMDFLLRNFTPLHYDDLINKRYQENTPYFHLSFDDGLSELYHI
                     IRPILIEKGIPATLFINDAFVDNHDLMYRYKVSLLIEHFTKESLLKCLHVLGKNKLNS
                     TNDPVQFLKQINYSEIPLVNSIAEHLGFDFSDYLRLHKPYLELSQLETMASEGFTIGA
                     HSVDHPNFSDIDAASQHDQVKLSVDYVRNTFNISNPAFAFPFSADGADAALLQNMQSA
                     YKISRSFGTAGLKRNTVENHIDRIPAEYLNWPLESIVKAEYHYYLAKKMIGK"
BASE COUNT         1757 a         1247 c         1334 g         1802 t
ORIGIN      
        1 gcggcgtatg tttttttgtt gaagaaacga tggtatagac aagcggaatt acgataagcg
       61 taagcatcag cgagaataat aatcctccga taatggcgat acccatggat acacgacttt
      121 ttgcagaggc gcccaacgct aatgcgatag gcaatgcacc taaaactgtt gcaaggctgg
      181 tcatcagaat gggacggaaa cgctgacgtg ccgcttccat ggcagcagca aatttatcga
      241 ggcccgcttc tttgcgctgg ttggcaaatt caacaataag gataccattt tttgttacga
      301 taccaatcaa cacgattata ccaatctgac taaaaatatt gatggtttga tcaaataaat
      361 agagcgaacc aaatgcacct gcaaatgcaa gcggaacggt gaacataacg atcagcggat
      421 cgaggaagct ttcgaactga gctgccaata ttaaatatac taataccagt gccattatca
      481 gtgcgaattg gagactgtta gagctttcag caaactcttt agaaggtccc gataatgcgg
      541 tagaaaaatc ttctgtcagc acatctttag caacagcatc catggcatca atgccatcac
      601 caatagtata accgggagca ggattggcca tgatggttgc agaaacatat ctgttataac
      661 gatataatga aggtggcgaa ctttgatcgg tgaggtgtac gagattatcc atttgtatca
      721 attcaccctt atcatttttt acatacgctt tgctgaggtc gaggggttca tcgcggttgt
      781 cgcgggttgc ctgaccaatt acctgatatt gcttattatt aaatataaag aaaccaaagc
      841 gctggccgct gaaatataat tgcaaagttt cagcgatatc tctaacagtt acaccaagtg
      901 cccttgcgcg atcacgatca atctcaactg ttaattcagg tttattgaat ttcaaattaa
      961 tgtccaccac ctgaaatgtt ggatcctgtg aggctttttc catgaattgc ggaatcactt
     1021 cccgcaggct ttcgaaatct ggtgcctgaa tgacatattg caccggtaaa cctgcacggc
     1081 ggtcgctgct gatggtttgt tcctgagaaa taaatgtgcg ggcgaaattg tatttcttca
     1141 caaccgctgc tatctcatca gcaatttctt gttgtgaacg ggtgcgctct tccggttcta
     1201 cgagggacac acgaataaat ccactgtttg cagcaatacc tgctccaaag gatggggcgg
     1261 tcacagcgat aatattttcc ttttcaggta gcgtatccac aatgtttatt aattcctgct
     1321 gaaaggcatc cattttttcg aatgaggtgc cttccggtgc tgtggaattg atcacgaagc
     1381 gacctttatc ttccattggt gccacttctt caggtagtaa taaaagacaa tacctaccca
     1441 aaaagattga accacccatt aacaacacta taaccattgc acgtctttgc atcagttttt
     1501 ccagtgccga tatataaacc ctatttagcc cttcaaaaaa tggttccgtg gttcgatata
     1561 accaattatg tctttcacga tgttttaaaa tacgcgcact catcatcggt gtaagtgtga
     1621 gtgaaaccaa tgctgatatc aatacagagc cggcaacggt aataccgaat tcacgaaata
     1681 acctaccggt gagcccttca agaaatacga taggcaaaaa cacaacaacc aaggtgatgg
     1741 tagttgaaat gatggcaaaa gtaatttcct tagagccttc atgtgctgcc tgcaacggtt
     1801 ttacaccgcg ttcaactttg gcataaatat tttccaatac cacgattgca tcatccacaa
     1861 caagtcctgt tgccagaacg ataccgagta gcgtcaacac attgatggag aacccggaaa
     1921 catacatgat gaaaaatgca cctatcaacg aaataggcac agcaacaacc ggtatcaggg
     1981 tagtacgcca gtcgcggagg aaaaggaata tgattaacac taccagtccg aatgcaatta
     2041 ataatgtttc ttttacttca tcaattgctt tgcggatgcc ggtggtggta tccaatgcaa
     2101 ttttatactg caggtcatcc ggtaattctt ttttaatctg atcgaagcgg cgataatatt
     2161 catctgctat ctcaatataa ttagaacccg gctgcggtgt gattgccact gccacctgcg
     2221 ggatgccgcc attgccacgc aatacggttc tttcgttctc aggcatgagt ttcgcctgac
     2281 caacatcttg cagtttaatt acctgtccgt cctgttcgag tataataagg tcgttgaatt
     2341 cctcttcagt ggtcatgcga ccgaaagtgc ggatagataa ttcagtagta tatccttcaa
     2401 tacgacctgt aggcagttca acattttctc tgttcaatgc atcgcgtacg tctgatggcg
     2461 tgagatgata tccggcgagt ttaacaggat cgagcaataa tttcatgctg tattttttct
     2521 caccccatac gcgcacttca cttacacccg gaatagtttg caatcgctcc ttaaatacat
     2581 tattggcaat ttccgataat tccaataatg atcgttgatc gctttgaatg gtcatggaca
     2641 ctatcggatt tgaatcggca ctagatttag caacaatagg cggatcacaa tccggtggca
     2701 gatttcgagc tgcctgactc acgcggtcgc gcacatcgtt tgctgcagct tcaagatcaa
     2761 catcgagcgt aaattcaaca gtaatcgtac ttcgaccatc tgatgatgta gatgataaag
     2821 aactgatacc ggcgatacca tttatttgtt cttcgagcac ttcggtgatt tgcgattcga
     2881 tgatatcggc attggcaccg gtataattag tggttacggt aatgatggga ggatctacgc
     2941 tgggaaattc gcggacgccc agaaatgagt aaccgataat tccgaataac agaattaaaa
     3001 tactgattac cgttgctaaa acggggcgat taatggatac agaagaaata ttcatgccat
     3061 caatcttttt tattttccac aataattggt ttcactgcgg caccgggtcg aaccgttaat
     3121 aatccggtgg taataatggt atcgccggga acaatacctt cagtgatctg agtagtagta
     3181 gctgtgcgaa taccggtttt gataggttgc acctttgccg ttccggcgcg cactacatac
     3241 aatgtttgtc ctgtgatggc tgggatgata gcctctgaag gtacttcaat tgcttcggga
     3301 atagtgccga gattaatggt gagttttgca aaagcaccag gaatcaaccg ctgctcggca
     3361 ttcaaagcaa tagcacgtac ttttatagac ctggtttgcg gatcgatttt tggttcgcga
     3421 gcatagacct tggcttcaaa tgtgtggtcg tcgccctcaa ccgtgaaatt taccgttgca
     3481 ttatttttta tggatgatgc atatttctcg ggaacagaaa attcaacttt caccatattt
     3541 gtttgctgca ggctggcgat caatgtggcc ggagaaacat atccaccttc tgaaatataa
     3601 cgcagtccga tggttccact aaatggagct gttactttcg ttttcgctat ttgcgtttct
     3661 aataattgtt tatcagcctc cagtttcaat aaataatttc ttgaggcatc atactcttct
     3721 ttagagatac caccggcatc taacaatttc ctcttacgtt gctcatcttc agtggcaagg
     3781 tctatagata aatttgtttt agtaagttgt gcctgcaatt ctctgtcatc aattgtaagt
     3841 aacaggcttc cactattaac agcatcacct tccttaaaat acaattttat aactcgaccg
     3901 gaagtttcgc tgcgaatttc cacttcctca tttgcaagaa tggtcccggt tgcctcgatg
     3961 tggttatcga gcgaagaggt tgttgcgata atagccttga cgggttgtgc aggaggcggt
     4021 ccggcaggtg ctgcggattg cgacgattct tttgatttgg aacaagcggc aagaagggct
     4081 accgcactaa ttaaaaataa aaatcttttg ctcatactat tttttcgtga aaacgccggc
     4141 attgctatcg ctgaaatgtg acatgccagt cggcttcgag ttgttttaaa tcttgttcga
     4201 aatatggtgt tacgatttgt ctttcttcta tggtgagttt ggggagttcg acttgtgtct
     4261 tattgagttt atatatcttg tcaataatac cacttttgat aattaggttc ctgaaagggc
     4321 ggataaaaaa ggttaatgtt ttgcgcaaag gatgttcgcg attgagtaac agttgttgca
     4381 gccatttact cttgacaccg gtggaggtat taaaggcctc accttgttga ctgaatgcat
     4441 gatattgaat gcctagatat gtcgataatc ttttataaag cgcatcagca tcagatttta
     4501 actgatcgag tgtgccgata aaaatctgct cctttggaaa actcagggtc caaactgaaa
     4561 gatgcctggc atacaaacta ccataacaat gacataggtt gtttttggcg gctatatcat
     4621 ttgataataa tctctcctgt tcaagagtta tggtatcggc aaaactgata tcgctgcgtt
     4681 catgtccgaa ttggacgcta tattgataat ttgagtatgc tctgtcaata ggatcacgga
     4741 gcataacaat gatgtgcata tccggattat atgcttttat gcgtgccgga gcatcatggt
     4801 ccattaacaa ataggtgtcg gctgtcgcaa ttattttttt tgactcgcct tcgaataaac
     4861 tatggaaata tttttctcct cgttgataca aatcaataag tgaaaaataa tgcacttctt
     4921 ttgtaatact gaattgaata tcgggatgat gctccatcca tgcaaaaaca gatgtagtcc
     4981 cgcttcggcc tgctccgata atgatggtat ttactttatt catttaccaa tcattttttt
     5041 tgcaaggtaa taatggtatt cagcttttac aatggactcc aatggccagt tgagatattc
     5101 tgcaggaatg cggtcgatat gattttcgac agtatttctt ttcaagcccg cagttccaaa
     5161 gctgcgagat atcttgtacg ctgattgcat attttgtaat aatgcagcat cagcgccatc
     5221 agcagaaaat gggaaagcga atgcgggatt tgaaatatta aatgtattgc gcacatagtc
     5281 cacactcagc ttaacctgat catgttgtga agcggcatcg atatcactga aatttgggtg
     5341 atcgacacta tgcgctccga tggtaaagcc ttcggaggcc atggtctcaa gctgagatag
     5401 ctccagataa ggcttgtgca atcgcaggta atcagaaaag tcgaagccga ggtgttctgc
     5461 aatactgttg accagcggaa tttcgctata attgatctgc ttcaagaatt gaacaggatc
     5521 gttcgtactg ttgagtttat tttttcccaa cacatgcaaa cattttaata aggactcctt
     5581 agtgaaatgt tcaatcaata aacttacttt ataacgatac atcaaatcat gattatcgac
     5641 aaaagcatca ttaataaata atgttgcagg aattcctttt tcaattaaaa tggggcgaat
     5701 gatgtgatat aattctgata aaccatcatc aaaactcaag tggaaatatg gagtgttttc
     5761 ctgataacgt ttatttatca gatcgtcgta atgcagtgga gtaaaattgc gcaacaaaaa
     5821 atccatgtcg ctttcaaatt cctgcacatt tcgccagcga tataaatgtt tgatgtgggg
     5881 cagatcatcg ttacttaccg tatgataaaa tggaaaaatg atctgttgac cggaggcctt
     5941 tataaaatcc ttcaaagcac gtcgctcata acgtttcctg aaagccgaga accagattga
     6001 tttcacagaa ggcattggaa gggtaaaaat aaggagaaag gagaaaggag aaaggagaaa
     6061 ggagaaagga gaaaggagaa aggggtgagt gggtgagtgg gtgagtgggt gagtgggtga
     6121 gtggtgagct tgcctcgccg
//
LOCUS       DAOQHY010000183         6089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_7105_length_6089_cov_5.616009, whole genome shotgun sequence.
ACCESSION   DAOQHY010000183 DAOQHY010000000
VERSION     DAOQHY010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7105_length_6089_cov_5.616009"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PLL28_12440"
     CDS             complement(<1..335)
                     /locus_tag="PLL28_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70178.1"
                     /translation="MLLTGREWKEGYRFGFGGQEQDDEVYRNGNLNTAEYWEYDTRLG
                     RRWNIDPVLKVFESPYVAFNCSPIFRVDVNGDNTDWYKEIGAEGCDYNSYKYFETAEG
                     TEGDPYIVDG"
     gene            complement(399..917)
                     /locus_tag="PLL28_12445"
     CDS             complement(399..917)
                     /locus_tag="PLL28_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70179.1"
                     /translation="MQTHNVSSVRDNKNQSYQQMKLQSNDSTVSFSVISKIDTSAEFD
                     THLISKNDVIYAQEVILEKNGKLIAIAWPQVDTSVDTVKNIAGKKYSFFSQIIQSIYL
                     DEYNNSQIILTIKYAAFNNASGERYYYFTADGKYLGELDCVREICDTIGDPILWELDE
                     PIMQDTRSLFDK"
     gene            complement(986..1963)
                     /locus_tag="PLL28_12450"
     CDS             complement(986..1963)
                     /locus_tag="PLL28_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70180.1"
                     /translation="MLLPGREWQEGYRFGFGGQEKVDEIHGRGNSINYKFRMHDPRIG
                     RFFAIDPLVGKYPYNSSYAFSENKVIAWTELEGLETFFAADGTKLGQVGSSTQVMVVS
                     AEVIEMKGGIENIQKNLSEINTAITDPKVQSQHKVAGFEHLNWYTKNSNDVGITEAEL
                     NIRAYMATLKVTENGGNGALDYDDKHMEKGIVGKFTNYSDHPYRGQKGGTAAGAYQIL
                     LGTFDLYKKAYPKDIIDFSPTSQDKIVLGIFNRTGALEYIKNGNYKMANDKLTGKPEQ
                     FSSLPGGSQSRITNEEFDKMILQNLSNELSGESNIATPKGQIIQSVPNN"
     gene            complement(2094..2717)
                     /locus_tag="PLL28_12455"
     CDS             complement(2094..2717)
                     /locus_tag="PLL28_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70181.1"
                     /translation="MERTTNMLILLFAYFLVGCLSINTNTIDEEDIYIQRIVYSKYDS
                     LPYTGKLERREEGSIIMIMNFKDGFPLGPWRGYYNYPNEVEKHGEFADIQLISKKSRD
                     LIGTDSIAIAFEKSRNNPSYYNLTIYILAQENSFISTNTPSNWELYNKIALQIIEDLH
                     LSFKLDYFELEYVDAIYLPTKEYSVRFNITNEKIVNSWIVQDGKILE"
     gene            complement(2721..>6089)
                     /locus_tag="PLL28_12460"
     CDS             complement(2721..>6089)
                     /locus_tag="PLL28_12460"
                     /inference="COORDINATES: protein motif:HMM:NF026945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 44 domain-containing
                     protein"
                     /protein_id="HNF70182.1"
                     /translation="TGIGECSSNPIETPYIPYIDPCLQDQIDLAINNAYAAYEQQIIE
                     LTESFKEAYIAQCMGNLSEDFTKSFSSSEYHYTLYYYDQAGNLTMTVPPAGVDILDAT
                     EMTAMESYRYGGGDEIVPNHTLETRYRYNSLNQLTMQSTPDAGESRFWYDALGRIVLS
                     QNAKQAHVGESGVDQQEFSYTRYDELGRIYEVGELQIDDELITSTYIKAAVIDQASLT
                     SALIAEGTVSEVTSTYYNQPFTLPGSPFGTSGQTNQRNRISAVTIEAEPDTDPDTYDH
                     ATHYSYDIHGNVHTLIQDNPSIGEIQAEERFKKVQYTYDLVSGNVMLVAYQDNMADQM
                     YHAYQYDADNRIYEVQSSRDGYIWEKEARYFYYRHGPLARVETGDLIVQGTDYAYTIQ
                     GWLKGVNAGALIPDRDMGKDGYDDASNTHKYIAQDAFGFTLGYFDDDYTQINTGAAYK
                     FLPNVSATNFDGAANSLFNGNIMSMSTALLRPLEAGDADGKHDKADVLGKVYSYDQLN
                     RIKSSSSYEKAMTTTAAFTWGGASTYAVNKWKTSYTYDGNGNILTMNRAGTGASGYDM
                     DQMTYHYYEGTNQLQYVGDAVADAAYTDIDIDDQAGIDSDPIATGTQHDIYTYDKIGN
                     LVSDLSEGIESIAWTVYGKIKSIAKSDDTNLEFAYDATGNRIMKKFTPASGPIVYTYY
                     VRDAQGNIMAVYTREEEGTSNTLDLSELHIYGSSRLGMVQTTVDMLASFTEDSYFYRT
                     LGEKRYELTNHLGNVLSVISDRRLAFDTNADGVINIYEADVISAQDYDPFGMLLPGRE
                     WKEGYRFGFGGLEQDYEISSRGNYFTAEFWEYDAMLGRRWNLDPIRKTHESPYAAFSN
                     SPLFLIDPTGADTLLYSKNTGTKLTTKINPEDKKNPIWIVDENAYNYDPSNPWLTATA
                     LVYQKGKNNPNKKIDGKNLRKNHPLKNIGWAAGSQVYEEDLLDMTDEFNEDVTDALFL
                     FGTIGKSYKRLLKNKLNTPEDIMDTKVEMYAKFASLVGPNRRYDLKSTKRAGLNEDAS
                     YDAEFIGEYSLYNGNLMHYDDYGNYAFGVWGHVYGLTLSRISSAADIAQRIDKGNNDP
                     ARDQFMVKTGYNNFPFFREMWLMDNMGAAESLNN"
BASE COUNT         1689 a         1320 c         1071 g         2009 t
ORIGIN      
        1 ccatccacaa tgtacgggtc accctctgta ccttctgctg tctcaaagta tttgtaacta
       61 ttgtaatcac agccctccgc ccctatttct ttataccaat cagtattatc cccattaaca
      121 tcgactctaa aaatcggact acaattaaag gctacatatg gggattcaaa cacttttaaa
      181 accggatcta tattccacct tctacccaat ctcgtgtcat actcccaata ctctgcggta
      241 tttagatttc cgttcctata aacctcatca tcctgctctt gtccaccaaa cccaaaccta
      301 tacccctcct tccattcgcg cccggtgagt agcatcccaa acggatggtg gtttcaaaga
      361 actgggttaa agttaaaaaa actaagatag ttttgccttt atttatcaaa aagagatctt
      421 gtatcttgca ttatcggctc atccaactcc cacaaaatcg gatcgccaat tgtatcgcaa
      481 atttcacgaa cgcaatccaa ctctcccaaa tatttcccat cggcagtgaa ataataatac
      541 cgctcaccac ttgcgttatt aaaagccgca tatttaattg tcaatatgat ttggctatta
      601 ttatattcat caaggtaaat gctttgaatt atttgagaga aaaaactata ctttttgccg
      661 gcgatatttt ttactgtatc aacactagta tcaacctgtg gccatgcaat tgctatgagt
      721 tttccatttt tctcgagaat tacctcttgc gcataaatga catcgttttt cgaaattaaa
      781 tgagtgtcaa attcagcact tgtatcaatt ttagaaatca cactaaaact gacagtcgaa
      841 tcattgcttt gaagcttcat ttgctgataa gactgattct tattatccct aaccgaggaa
      901 acattgtgag tttgcaaagt atgaactttg ttcctacagc tacatgagta caccaagaga
      961 agaaaaacga gcgttgtttt cataattaat tgttaggaac tgactgtatt atttgccctt
     1021 tcggagtcgc aatatttgat tctccgctca attcattact caagttctgc aaaatcattt
     1081 tatcaaactc ttcatttgtt attctgcttt ggcttccacc tggcaatgat gagaactgtt
     1141 caggttttcc agtcaatttg tcgtttgcca tcttataatt tccgttcttg atatattcaa
     1201 gagctccagt tcgattaaag atacccagca caattttgtc ttggcttgtt gggctgaaat
     1261 caattatatc ttttggatat gcttttttat aaagatcaaa tgttccaagt agaatttgat
     1321 atgctcccgc cgcagtcccg cccttctgac ctcgataagg gtggtcggaa taattggtga
     1381 atttaccaac aattcctttt tccatatgct tatcatcgta atccagtgca ccatttccgc
     1441 cattttcggt taccttcaaa gttgccatat atgctcgtat gttcagttca gcttcggtta
     1501 taccaacatc attgctattt tttgtgtacc aattaaggtg ttcaaaccca gctactttgt
     1561 gttgtgactg aactttagga tcagtaatcg ccgtattaat ttcgcttaga ttcttttgaa
     1621 tgttttctat tcctcctttc atttcaataa cttctgcact caccaccatt acctgagtac
     1681 tactgccgac ctgacccaat ttagttccat cggctgcaaa aaacgtttcc aaaccctcta
     1741 attcagtcca agcaattact ttgttttcag agaatgcata agatgaattg tagggatatt
     1801 taccaaccaa aggatcaatg gcaaaaaatc ttccgatcct tggatcatgc attcgaaatt
     1861 tatagttaat ggagttccct cttccgtgaa tttcatctac cttttcctgt ccaccaaatc
     1921 caaacctata cccctcctgc cattcgcgcc ccgggagtag catcccaaat ggatggtggt
     1981 ttcaaagaac tggattaaag attgaaacca atatttctta ggttaggata aaaataacac
     2041 atttattaat aaaataaaaa taagatccca attcccataa gaacattgat accttattcc
     2101 aagatttttc catcttgaac aatccaagaa ttaacaattt tctcattagt tatattaaat
     2161 cttaccgaat attcttttgt gggtaaatag atcgcatcta catattctaa ttcgaaataa
     2221 tctaatttga acgagaggtg cagatcttca atgatttgca atgcaatttt gttatataac
     2281 tcccaatttg aaggggtgtt tgttgaaata aaactatttt cttgtgcaag tatgtaaatg
     2341 gttaaattat agtaagaagg attatttcgg ctcttttcaa atgcaattgc aatggagtct
     2401 gtaccaatta agtcgcgaga ttttttggaa attagttgta tatctgcaaa ctcaccatgt
     2461 ttctccactt catttgggta attataataa cctctccatg gtcctagagg aaacccatct
     2521 ttaaagttca ttatcataat aatgctgccc tcctcccgtc tttccaattt accggtatat
     2581 ggcaaggagt cgtatttgct atacacaatc ctttggatat aaatgtcttc ttcatcaatt
     2641 gtgttagtat taatggataa gcagcctact aaaaagtagg cgaaaagcag aataagcata
     2701 tttgttgttc tttccacaac tcaattattt aaagattctg cagctcccat attatccatt
     2761 agccacattt cacgaaaaaa tggaaagttg ttatacccag tctttaccat aaattggtcg
     2821 cgtgccgggt cgttatttcc tttatctatt ctttgtgcaa tatccgctgc acttgagatt
     2881 ctagagaggg taagcccata aacatggccc caaacgccaa aagcgtaatt gccgtaatca
     2941 tcataatgca ttaaatttcc attatataat gagtattccc cgatgaattc agcatcataa
     3001 gacgcatctt cattgagtcc agccctttta gtgcttttca aatcatagcg tctattcggc
     3061 ccaactaaac ttgcaaattt tgcatacatt tccacctttg tatccattat gtcttccggt
     3121 gtatttaatt tattcttcaa taaccgttta tatgatttac caatagtccc aaataaaaat
     3181 aacgcatcag tgacatcttc attaaattca tctgtcatat ccaataaatc ttcttcgtaa
     3241 acttgtgaac cagcagccca accgatattc tttaatggat gattttttcg caagtttttg
     3301 ccgtcaattt tcttattagg attgttttta cctttttgat aaactagagc tgtagccgtt
     3361 aaccaaggat tgctcgggtc gtaattatag gcattttcat cgacaatcca aatgggattc
     3421 tttttatctt cagggtttat tttggttgta agtttagttc cagtgttttt cgaatacaac
     3481 aaagtgtcag ctcctgtagg atcaatgaga aacaacgggc tatttgaaaa tgcggcgtac
     3541 ggggactcat gtgttttcct tatggggtct aggttccacc ttctacctaa catggcatca
     3601 tactcccaaa actcagccgt aaaataattt ccacggctgg atatttcgta atcttgttcc
     3661 aaaccaccaa acccaaacct atacccctcc ttccattcac gccccgggag tagcatccca
     3721 aatgggtcgt aatcctgagc ggagataaca tctgcctcat aaatgtttat gacgccatcg
     3781 gcatttgtat caaaagctaa gcggcggtca ctaataactg aaagaacgtt tccaaggtgg
     3841 ttggttaatt cataacgctt ttcaccaagg gttctgtaaa aataactatc ttctgtgaat
     3901 gatgcaagca tgtctactgt tgtttgcacc atgcctagac gactactgcc ataaatatgt
     3961 aactcactca gatccaaagt attggatgtg ccctcttctt cccttgtata tacagccatg
     4021 atgtttcctt gggcatcgcg cacatagtat gtataaacga taggcccgct tgccggtgtg
     4081 aatttcttca tgatgcggtt accggttgca tcataggcaa actccagatt ggtatcatca
     4141 gatttggcaa tagacttaat ttttccgtaa accgtccacg caatcgattc aattccttcg
     4201 ctaaggtctg ataccagatt tccaatctta tcgtaggtat agatatcgtg ctgagtgcct
     4261 gtagcaattg ggtcgctgtc tattccggct tggtcgtcga tgtctatatc tgtgtatgct
     4321 gcgtcagcaa cagcatcgcc aacatattgc aattgattgg tgccctcata gtagtgatag
     4381 gtcatttgat ccatatcata tcctgatgcg cctgttccag cacggttcat tgtcaggata
     4441 tttccattac catcgtaggt atatgatgtt ttccatttgt taacagcata agtgcttgca
     4501 ccgccccaag taaacgcagc agttgtggtc attgcctttt cataactgct actggattta
     4561 atgcgattca attgatcgta agaatacacc tttcctaaaa catcggcctt atcatgcttg
     4621 ccatcggcat cacctgcttc cagtggacgc aaaagtgctg tgctcatgga catgatgttg
     4681 ccattgaata agctgtttgc tgcaccatca aaattagttg cactcacgtt tggaaggaat
     4741 ttgtatgcag caccggtatt tatttgggta taatcatcgt cgaaatagcc taatgtaaat
     4801 ccaaatgcat cctgtgcgat gtatttatgt gtatttgagg catcatcata tccatcctta
     4861 cccatgtctc tatcaggaat aagcgctccg gcatttactc ctttcagcca accttgaatg
     4921 gtataggcat aatcggtacc ttgcacgatc aggtcaccgg tttcgacccg cgccaaaggt
     4981 ccgtgtcggt agtagaaata cctcgcctct ttttcccaaa tgtatccgtc gcgcgaggat
     5041 tgcacttcat aaattcgatt atcggcatca tattgatagg catgatacat ctgatcggcc
     5101 atattatctt gataggcgac cagcattaca tttccgctta caaggtcata ggtatactgc
     5161 acttttttga atcgctcttc agcctgaatt tcaccaatag aaggattgtc ctggatcaac
     5221 gtatgcacat tgccatgaat atcgtagcta taatgcgtgg catggtcata agtatccgga
     5281 tctgtatcgg gttcagcttc tattgttacg gcgctgatcc tgttgcgttg gttagtttga
     5341 ccggatgtac caaatggtga acctggtaaa gtaaatggtt gattgtaata ggtgctggta
     5401 acttcagata cagttccttc agctataaga gcggaagtca aagatgcctg atctataact
     5461 gctgccttga tatatgtgga ggttattaat tcgtcatcaa tttgcaattc accaacttca
     5521 tatatacggc ctagttcatc ataccgtgta tagctgaatt cttgttgatc aacaccggat
     5581 tcaccaacgt gggcttgttt tgcgttttgg gataagacta ttctaccaag tgcatcatac
     5641 cagaatcggc tttcacccgc atcaggagtt gattgcatgg tgagctgatt tagactatta
     5701 tatcgatatc ttgtttctaa tgtatggttt ggcacaattt catcaccacc gccgtatcga
     5761 taactttcca ttgcagtcat ttcagtggcg tccaatatgt ctactccggc tggaggaact
     5821 gtcatggtta gatttcctgc ctggtcataa taataaagtg tataatgata ttcggaagag
     5881 ctgaatgatt ttgtaaagtc ttcagacaaa ttacccatac attgtgctat ataagcctct
     5941 ttgaatgact ccgttaattc aataatctgc tgctcatagg ctgcataggc gttgttaata
     6001 gccaaatcta tttgatcttg gaggcaaggg tcaatatatg gtatatatgg tgtttctatg
     6061 gggtttgatg aacattcgcc tatgccggt
//
LOCUS       DAOQHY010000184         6074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_7128_length_6074_cov_6.286592, whole genome shotgun sequence.
ACCESSION   DAOQHY010000184 DAOQHY010000000
VERSION     DAOQHY010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6074
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7128_length_6074_cov_6.286592"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(409..1359)
                     /locus_tag="PLL28_12465"
     CDS             complement(409..1359)
                     /locus_tag="PLL28_12465"
                     /inference="COORDINATES: protein motif:HMM:NF022779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HNF70183.1"
                     /translation="MHDAAAFEAFYRQEIVPDLDRLEKIRKEQLKRVTFMWSSAIISL
                     VLAFLLTMPVLVVIFLFIALGVYFYLFGFNRKRVDFKTEYKRVVIGKLATYISDQLVF
                     TPNIFIPESTYNQSKIFLSDPDIYTGEDLVTGKIGNTQIQFCELHTKDRQTDSRGRTT
                     YVTIFKGIFFIADFNKHFNGQTYVLSDFGERFLGAFGRLMQSINVGRPDVVRLEDPEF
                     EKEFVVYSSDPVEARYILSPAMMQRLLEYRKKTGSKIQCSFLNNNIYLAMPMKENLFE
                     PSLRKSVMTMADVEGFYKDLLFCTSIVDELDLNTRIWTKE"
     gene            complement(1427..2098)
                     /locus_tag="PLL28_12470"
     CDS             complement(1427..2098)
                     /locus_tag="PLL28_12470"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF011369.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HNF70184.1"
                     /translation="MKKRSLVAFAVIIGVLLIDQLLKFYIKTNFTLGGGFDILGLSWA
                     KIHFVENEGMAFGLELGGTYGKLLLTLFRLLAVGGIGYYLYTLIRDKANFGLIISIAL
                     VCAGAMGNIFDSVFYGIYFSESTFTQVAEFMPAGGGYTTVLHGHVVDMLYFPIADGHW
                     PEWMPFIGGKYYHFFRPVFNIADSAITTGVLSILIFQRRIFASPEMESAKEKPTIAEE
                     TAREE"
     gene            complement(2103..3233)
                     /locus_tag="PLL28_12475"
     CDS             complement(2103..3233)
                     /locus_tag="PLL28_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNF70185.1"
                     /translation="MAKKIVKATKSSASKSAPTKTKTAAKKAAPAPKKVVKPVAKKAA
                     PKAAKKTTAKPVAKKAAPKAPKKAVAKPVAKKTAAKPQPKAKAPAKKAVAVKTPLKPV
                     VKKEVPKAPVKKEAPKAPVKKEVAKKPEAKKPEVKKPEVKKPEVKKVEVKKPEAAKKV
                     APVEKTPIKKAEKQPEVKKPEVKKPEPVKVVAPEVQPVATKKTAAKKSTKKNAPPPPP
                     APTYDNPEIKLGPVKILPPKRELNPQPVKVTSTVLDNRTRYSDEELQEFKELITGKLE
                     AARNELKYLQEQISRSSEMGDDSDARFKGLEDGTSTSEREYLSQMASRQITYIGHLEK
                     ALIRIENKTYGICRETGKLISKERLRAVPHATLSIEAKQGKG"
     gene            complement(3238..>6074)
                     /gene="ileS"
                     /locus_tag="PLL28_12480"
     CDS             complement(3238..>6074)
                     /gene="ileS"
                     /locus_tag="PLL28_12480"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNF70186.1"
                     /translation="HNYIETLWNLLKRLYDKGLLYKGFTIQPYSPAAGTGLSSHELNQ
                     PGTYKPVKDTTATAMFRILPEHIPAHLMTQHAEVFFLAWTTTPWTLPSNTALAVGANI
                     TYVQIDTFNPYTQKPISVILAKDRVPAYFKDEQKVHALISEEPAGWKPVSEFTGKQLE
                     GIRYEQLLPYHQPTNGDAFRVITGDFVTTEDGTGIVHIAPSFGSDDFRVAKQNGIGSL
                     TLVDKEGKFVDGMGDLSGRFVKNYKDEEKWENPDVIIAIKLKESNRAFRVEKYEHNYP
                     HCWRTDKPVIYYPLDSWFIRTTAAKERLIALNKTINWKPASTGEGRFGQWLENLVDWN
                     LSRSRYWGTPLPIWRSEDGAEELCIGSFEQLQKEVDRARDLNPHLPADWNAASNFDPH
                     RPFVDEIVLRSASGKPMRRESDLIDVWFDSGAMPFAQWHWPFENTEVFDRKFPADFIA
                     EGVDQTRGWFFTLHVIATLINDSVAYKNVISNGLVLDKNGNKMSKRLGNAVDPFHTIN
                     NYGADATRWYMITNAQPWDNLKFDTDGIVEVQRKFFGTLYNTYAFFALYANVDEFRFE
                     GNEIPVSNRSELDRWVISLLNSLTAQVTEALNDYEPTRAGRAIQDFVGDHLSNWYVRL
                     SRRRFWKSDDSADKQAAYQTLYTCLDTISRLMAPIAPFMSEWLFRNLNGVTGKHQAES
                     VHLSTFPVADPTLIDRDLEDSMQLAQDISSTVLSLRKKQNIKVRQPLSKIMIPVLSDT
                     TRRHIERVAELVKSEVNIKEIEFISEDSGVITKKIKPNFKALGPKLGGDMKSAAGLIS
                     GFSQQQISELERNKEIILDLGNKSFTLSSEDVEILSEDIPGWLVAPAGTLTVALDITV
                     TDALLFEGHARELVSQLQRMRKDMNLAITDRISVTYHTGAELSQAIEDYKQYICAEIL
                     ADSLETSDGLSDTGKISVNDIEISIQIKKV"
BASE COUNT         1523 a         1391 c         1350 g         1810 t
ORIGIN      
        1 gtatcaggta tcaagtatca ggtatcaagt atcaggtatc aggtatcagg tatcaggtat
       61 caagtatcag gtatcaagta tcaggtatca ggtatcaagt atcaggtatc aagtatcagg
      121 taaatgtata ttcacatcgc atacccgatc ctcacgtatg cacaacactc acgagtcacg
      181 agtcacgagt cacgatatta gtatctagaa ccaagctcgc ctcgcctccg gcgggtatct
      241 agtagctaga cttggtatca ggtatcaggt atcaagtatc aggtaaatgc atgttcacat
      301 cgcatactag atcctcgaat acgaacaaca ctcacgagcc acgactcacg actcacccac
      361 tcacccctct ttcctttttc ctctctcctt tttcctcatt tccacccctc attccttcgt
      421 ccagattctc gtattcagat cgagttcatc aacaattgat gtgcagaaaa ggaggtcttt
      481 ataaaatcct tccacatcag ccatggtcat taccgatttg cgaagtgaag gttcgaataa
      541 attttccttc attggcatag cgaggtaaat attattattt aggaaggaac attgaatttt
      601 ggaaccggtt ttctttctgt attcgagcag gcgttgcatc attgccggcg aaagaatata
      661 gcgcgcttca acagggtctg aagaataaac gacgaattct ttttcaaatt ccggatcctc
      721 aagtcgcact acatccggac gtccaacatt tatactttgc atcaggcgac cgaaagcacc
      781 aagaaagcgt tcaccaaaat cgcttaacac ataggtttgt ccgttgaaat gtttgttgaa
      841 gtcggctatg aaaaatatgc ctttgaaaat ggtaacataa gttgtgcgtc cgcgactatc
      901 ggtttgacga tcttttgtat gcagttcaca aaattgaatt tgtgtattgc cgatttttcc
      961 cgttaccaga tcttcaccgg tataaatatc cggatcgctt aagaatattt tactttgatt
     1021 gtaagtgctt tcaggaataa aaatattggg tgtaaaaaca agttgatcag aaatatatgt
     1081 tgcgagttta ccaattacta cacgcttata ttctgtttta aaatctacac gtttacgatt
     1141 gaatccgaat aaataaaaat atacacctaa ggcaataaaa aggaatatga ctaccagcac
     1201 cggcatagtc aataaaaatg cgagcaccaa cgaaatgata gcagatgacc acataaaggt
     1261 cacgcgcttt aattgctcct ttcttatttt ttcaagtcgg tcgagatcag gtactatttc
     1321 ctggcggtag aatgcttcaa atgcggctgc gtcatgcatg gcacaaagat agtgaaaccc
     1381 ttatagggaa atcaacagct taaacagaga tgggatggag cgtgacttat tcttcgcggg
     1441 cggtttcttc agcgattgtg ggtttttcct ttgcggattc catttccgga gaagcgaaaa
     1501 tgcgacgctg aaaaatgagg atagagagta cgccggtggt gatggcgcta tcggcaatat
     1561 tgaaaacggg acggaagaag tgataatatt ttcctccaat aaatggcatc cattccggcc
     1621 agtgtccatc ggcaattgga aaataaagca tgtccactac atgtccatgc aataccgttg
     1681 tataaccacc tcctgcaggc ataaattctg caacctgtgt aaaggtgctt tcagagaaat
     1741 agatgccgta aaaaacgctg tcgaaaatat ttcccatcgc cccggcacaa accagcgcaa
     1801 tgctgatgat gagaccaaaa tttgctttat cgcgaataag cgtatataaa taataaccaa
     1861 ttcctcctac agcaagtaag cggaataacg taagcagcag tttgccataa gtgcctccga
     1921 gttcaagtcc gaaagccata ccttcattct cgacaaaatg tattttcgcc catgaaagtc
     1981 ccagaatatc aaatccgccg ccgagtgtaa aattggtttt gatataaaat ttcagcaact
     2041 gatcgatcag cagcacacca ataattacag cgaaggcaac gagacttctt tttttcacgc
     2101 tatcagcctt ttccttgttt agcttctatg ctcagcgtag catgcggaac tgcgcgaaga
     2161 cgttcttttg aaatcagttt tcctgtttcg cgacagatgc cgtaggtttt attttcaata
     2221 cggataagcg ctttttcgag gtggccgata taggtgatct gacgagatgc catctgactg
     2281 agatattctc gttcagaagt agaggtgcca tcctccagac ctttaaaacg tgcatcagaa
     2341 tcgtcgccca tctcacttga acgactgatt tgctcctgca agtattttaa ttcattacgc
     2401 gcagcttcaa gtttaccggt gatcagttct ttgaactcct gcaattcttc gtcgctataa
     2461 cgtgtgcgat tatccagtac ggtggaagta acttttacgg gctgtggatt taattctcgt
     2521 tttggaggga gaatttttac cggtccgagt ttgatttcag gattatcgta tgttggcgca
     2581 ggtggtggcg gcggtgcatt tttctttgta gatttttttg ccgcagtttt tttagtggca
     2641 acaggctgca cttcaggagc aacaactttc accggttcag gcttctttac ctccggtttt
     2701 ttcacttcag gctgtttttc cgcctttttt attggtgttt tttcaacagg agctaccttt
     2761 ttcgcagctt ccggcttctt cacttccact ttttttacct caggtttctt aacctcaggc
     2821 ttttttactt ccggcttctt ggcttccggt ttttttgcaa cttctttctt cactggcgct
     2881 tttggtgctt ctttcttcac gggtgcttta ggaacttctt ttttaactac cggtttaagg
     2941 ggagttttta cggcaacagc tttctttgca ggtgctttcg cctttggttg aggtttggca
     3001 gctgtttttt tagctaccgg ttttgccacg gcttttttag gtgcttttgg ggctgctttt
     3061 ttagctacag gcttagctgt tgtctttttg gcggcctttg gagcagcttt cttggcaaca
     3121 ggtttcacta ccttcttagg ggcaggagca gcctttttgg cagcagtctt cgttttggtt
     3181 ggggcgcttt tcgacgccga tgacttagtg gctttcacaa ttttcttcgc catggcacta
     3241 tacttttttg atctgtatac ttatttcaat atcattaacg gagatcttac cggtatctga
     3301 caggccgtcg gatgtttcca gggaatccgc taaaatttcg gcgcaaatat actgcttata
     3361 atcttcgata gcttgcgaaa gttccgcccc tgtatgataa gtaacggaaa tacgatctgt
     3421 aattgccaga ttcatatcct tgcgcatgcg ttgcaactga cttaccagct ctctggcgtg
     3481 tccttcaaac aacaaagcat cggtgacagt gatatccagg gctaccgtaa gtgttccagc
     3541 aggagctact aaccaccccg ggatgtcctc actcagaatc tctacatctt ctgacgataa
     3601 agtaaacgat ttattgccaa gatccagtat gatttccttg tttctttcca gctcggaaat
     3661 ctgctgctgt gaaaagccgg aaatcaagcc cgcagcggat ttcatgtcgc cacccaactt
     3721 cgggcccagc gccttgaaat taggttttat ctttttggtg atgacaccgc tgtcttcgct
     3781 gataaattct atctccttga tgttcacctc gcttttcacc aactcggcta cgcgctcaat
     3841 atgtcgacgg gtggtgtcac tcaacaccgg gatcatgatc ttgctcaaag gctgacgcac
     3901 cttgatgttt tgctttttgc gaagcgacaa tacagtgctg gagatgtctt gcgccagttg
     3961 catgctatct tccagatccc tgtctatcaa agttggatcg gcaacaggga atgtagacag
     4021 gtgaacggat tcagcctgat gtttgccggt aacgccgtta aggttgcgga acagccactc
     4081 cgacataaat ggagcaatag gtgccatcaa tcggctaatc gtatccagac aggtgtatag
     4141 agtctggtaa gccgcttgct tgtcggcgct gtcatcgctt ttccagaaac gtcggcgact
     4201 caaacgcaca taccaattgc tgaggtgatc gcccacaaag tcttgtatgg cgcggcctgc
     4261 acgtgttggt tcgtagtcgt tgagggcctc ggtaacttgt gcagtgagtg agttaagcag
     4321 gctgataacc cagcgatcca gctcactgcg atttgatacc ggaatttcat ttccttcaaa
     4381 tcggaactca tcaacattgg catacaatgc gaagaatgcg taggtgttat agagtgtgcc
     4441 gaagaacttg cgctgcactt ctacgatgcc atcggtatca aatttcagat tatcccaagg
     4501 ctgtgcattg gtgatcatat accagcgggt ggcatcggca ccataattat taatagtatg
     4561 gaaaggatca acggcattgc ccaggcgttt cgacattttg ttgccgtttt tgtctagcac
     4621 taagccattg ctgataacgt ttttatatgc aacagaatca ttgataagcg ttgcaattac
     4681 atgcaatgtg aaaaaccatc cgcgcgtttg atcaacgccc tctgcgataa agtcggcggg
     4741 aaattttctg tcaaaaactt ctgtgttctc aaaaggccag tgccattgcg caaaaggcat
     4801 cgcacccgaa tcaaaccaaa catctatcag gtcgctttcg cggcgcatcg gttttcctga
     4861 agcactgcgc aacacaattt catctacaaa cggacgatgc gggtcaaaat tgcttgccgc
     4921 attccagtcg gcgggaagat gaggattgag gtcgcgggcg cggtctactt ctttctgtaa
     4981 ttgttcgaag gagcctatgc aaagctcttc agcaccatcc tcgctgcgcc agataggaag
     5041 tggagttccc caatagcgtg agcgactgag gttccagtct accaggtttt ccagccattg
     5101 accaaaacga ccttcgcctg tgcttgcagg cttccagttg atggttttat tgagggctat
     5161 caatcgctct ttcgcggcag tagtgcggat aaaccacgaa tcaagcggat agtatattac
     5221 gggtttatct gtcctccagc aatgcggata gttgtgttca tacttctcaa cgcggaaggc
     5281 gcgattgctt tcttttagct ttatcgcaat aataacatcg gggttttccc atttctcttc
     5341 gtccttataa ttcttcacaa agcgaccgct cagatcgccc atgccgtcga caaattttcc
     5401 ctccttgtcc accaatgtta atgagcctat accattctgc tttgctacgc ggaagtcgtc
     5461 agaaccgaaa cttggcgcaa tatgcacaat gccggtaccg tcttctgtgg ttacaaaatc
     5521 gccggtgatc acacgaaatg catctccgtt tgttggctga tgataaggca atagttgctc
     5581 ataacgaatt ccttcaagtt gctttcctgt gaattccgac accggtttcc atcccgcagg
     5641 ttcctcagaa attaatgcat gtactttttg ctcatcttta aaataagcag gaacgcggtc
     5701 tttagccaga attacgctaa taggtttttg tgtatatggg ttgaaggtgt caatctggac
     5761 gtaggtaata tttgctccaa ccgctaaagc tgtattgcta gggagggtcc atggtgtggt
     5821 ggtccaggcc aggaagaaca cttctgcgtg ctgcgtcatc aaatgtgccg gaatatgttc
     5881 gggcaggatg cggaacatgg cagttgccgt ggtatccttc accggtttat aggtgcccgg
     5941 ttggttgagt tcgtgtgagc tcaatcccgt tcccgcagca ggagaataag gctgaatggt
     6001 gaatcctttg tacagcaaac ctttgtcgta cagtcgtttc agcagattcc acagtgtttc
     6061 gatatagtta tggt
//
LOCUS       DAOQHY010000185         6068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_7144_length_6068_cov_5.854482, whole genome shotgun sequence.
ACCESSION   DAOQHY010000185 DAOQHY010000000
VERSION     DAOQHY010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7144_length_6068_cov_5.854482"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="PLL28_12485"
     CDS             <1..742
                     /locus_tag="PLL28_12485"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNF70187.1"
                     /translation="WIPDQPGGDYILVNIPEYTMHIYEADTQAWEMNVVVGKSSTSTT
                     IFNDELEYIVFSPYWVPPPSILNNEILPSLKKDPNYLQKQNMEAFNPSTREVINVSKV
                     DWSKYTSMPYSIRQKPGNSNALGWVKFLFPNEHNIYFHDTPSRDLFVRESRGFSHGCI
                     RLQEPKKLAQYLLRNDSTYTEQVIDSLYYLGRENYVKLENKIPVYIIYLTAWVDDNGL
                     VQFRKDIYGHDAKLEQTMYGAPEVDLAN"
     gene            complement(864..5186)
                     /gene="rpoC"
                     /locus_tag="PLL28_12490"
     CDS             complement(864..5186)
                     /gene="rpoC"
                     /locus_tag="PLL28_12490"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNF70188.1"
                     /translation="MPFKKDIKIKSDFTKITIGLASPDSILERSYGEVLKPETINYRT
                     YKPERDGLFCERIFGPIKDYECYCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIK
                     LVVPVVHIWYFKSLPNKIGYLLGLSSKKLESIVYYERYCVIQPGPLTEGVMINGELKS
                     YNTHDLITEEEYLDILEALPRENQMLDDNDPNKFIADMGAPAVFTMLSRIQLDDLSAA
                     LRHQAGTETSQQRKAEALKRLSVVEMFREASGRYENRPEWMIVQYLPVIPPELRPLVP
                     LDGGRFASSDLNDLYRRVIIRNNRLKRLIEIKAPEVILRNEKRMLQEAVDSLFDNSRK
                     SNAVKAEGGRALKSLSDVLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKLHECGLPK
                     DMAAELFKPFIIRKLIERGIVKTVKSAKKLVDRKEPVIWDILENILKGHPVMLNRAPT
                     LHRLSIQAFQPKLIEGKAIQLHPLVCSAFNADFDGDQMAVHVPLSNAAILEAQLLMLS
                     AHNILNPQNGNPITVPSQDMVLGLYYTTKGKKSTADDVVKGEGKAFYSAEEVIIAYNE
                     GKVDLHAYIKCKVNVLENDVIVSKVIETTVGRVMFNEVVPAEVGFINALLSKKAIAKV
                     IGEVIRKTDVPRTAKFLDDIKDLGFRSAFRGGLSFNISDLVEVENKSDLVAAAQSEVD
                     EVISNHMYGVITQNERYNQIIDIWSRLNTRLTGDLINRLTSDKKGFNSVFMMLDSGAR
                     GSKEQIRQLGAMRGLMAKPRKSGASGEGGIIENPILSNLKSGLSVLEYFISTHGARKG
                     LADTALKTADAGYLTRRLVDVAQDVVITEEDCGTLRGISIAALKDNEDIVEPLSERII
                     GRTSLHNVFDPLTDELIVAAGELITDRIAARIDETAIEFVEIRSVLTCESKKGVCVKC
                     YGTNLANNRVAQKGDAVGIIAAQSIGEPGTQLTLRTFHVGGVAGSIDTESKLVAKFDG
                     KAEFDGLRFVETKNEEGKKIRVVIGRTGEIKIIEPKTGKMLISNNIPYGSILSVSDGD
                     EMKKGDVMCSWDPFNAVIISEFAGFVSYEHIIEGITYREESDEQTGHREKVISESRDK
                     TKIPTLIIKDKKGNEVKGYNLPVGAHIAVEEGEEVKSGQTIAKIPRALGKVRDITGGL
                     PRVTELFEARNPSNPAVVAEIDGVVAFGKIKRGNREIEIESRDGEKRKYLVPLSKQIL
                     VQENDYVYAGMPLSDGAITPSDILAIKGPYAVQEYIVNEIQEVYRLQGVKINDKHIET
                     IVRQMMRKMTITDAGDTRFLEDSSVDKLDFLEENDRIFDKKVVTDAGDSTKLKAGQIV
                     TLRQLREENSQLKRADKKPVEYRDAIAATATPLLLGITRASLGTQSWISAASFQETTK
                     VLSTASISAKTDYLMGLKENVIVGHQIPAGTGQRELLDLVIGSKEELDELNAAKKRKN
                     NDGE"
     gene            complement(5219..>6068)
                     /gene="rpoB"
                     /locus_tag="PLL28_12495"
     CDS             complement(5219..>6068)
                     /gene="rpoB"
                     /locus_tag="PLL28_12495"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611286.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNF70189.1"
                     /translation="ADTDDKVRMILHNYGIKVNEELGRYKREKFNISVGDELPTGVLK
                     LAKVFIANKRKLRVGDKLAGRHGNKGIVARIVRQEDMPFLEDGTPVDIVLNPLGVPSR
                     MNLGQIYETVLGWAGLKLGVKFATPIFDGATLAEIDEQILKAELPKLGSTYLFDGETG
                     ERFHQKATVGVIYMIKLNHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVW
                     ALEAYGASNILQELLTVKSDDIIGRAKTYEAIVKGENLPTPQIPESFNVLVHELRGLA
                     LDLKFE"
BASE COUNT         1680 a         1399 c         1193 g         1796 t
ORIGIN      
        1 atggatcccc gaccagccgg gtggcgatta tattcttgtt aatataccgg aatataccat
       61 gcacatttat gaagcagata cgcaagcatg ggaaatgaat gtggttgtag ggaaatcaag
      121 cacgagcacc accattttta acgacgaatt ggaatacata gtgttctcac catactgggt
      181 ccctccaccg agcatattaa acaacgagat acttccatca cttaaaaaag acccgaacta
      241 tttgcaaaag caaaatatgg aagcttttaa tccctctacc cgggaagtaa taaatgtaag
      301 caaggtagac tggagcaaat acacctctat gccatatagt attcgtcaaa aaccaggtaa
      361 tagcaatgct ttaggatggg tgaaattttt attccccaac gaacataata tttatttcca
      421 cgacacacct tcacgtgatt tgtttgtcag agaatcgcgc ggatttagtc atggatgtat
      481 tcgtttacag gaacctaaaa aactggctca atatttattg cggaatgata gcacctacac
      541 cgagcaagtg atcgactcac tatattattt aggcagagag aattatgtga aactggaaaa
      601 taaaattccg gtatatatta tttacctgac tgcctgggtt gatgacaatg gtctggtaca
      661 attcagaaaa gatatttatg gtcacgatgc caaactggag caaacgatgt atggagcacc
      721 cgaagtagat ttagcaaatt aaattacttg cctcgccctc aaattcaatt tggttattgg
      781 ggtattgaac aatacaaaac gaggcgccag aaataaaaaa agccctccgt aaacggaagg
      841 ctttttatta tttgatgagg tgcttattca ccatcattat ttttgcgttt tttagccgca
      901 ttcaactcat ccagctcctc tttgctaccg atcacaagat caagcaattc gcgctgacct
      961 gttccggcag ggatctgatg tccaacgata acgttttctt tcaagcccat gagataatct
     1021 gtttttgctg aaattgacgc tgtgctcaac accttggtag tttcctggaa cgacgctgca
     1081 gatatccagc tctgagttcc caaagatgct ctggtaatac cgagcaacaa tggtgttgca
     1141 gttgctgcaa tagcatcgcg atattcaaca ggttttttat cggcacgctt caattgtgaa
     1201 ttttcttcac gcaattgacg caatgttacg atctgaccgg ctttcaattt tgttgaatca
     1261 cctgcatcag taactacctt cttgtcgaag atacgatcgt tctcttcaag gaagtcgagt
     1321 ttatcaactg atgaatcctc aaggaatcta gtatcacctg catctgtgat ggtcatctta
     1381 cgcatcatct gacgaacaat cgtttcgata tgcttgtcgt tgatcttcac cccctgtaag
     1441 cgatatactt cctgaatttc gtttacgata tactcctgaa ctgcgtatgg acctttgatg
     1501 gcaagaatgt ctgaaggtgt aattgcacca tccgacaatg gcataccggc atatacgtag
     1561 tcattttcct gaacaagaat ctgcttagaa agtggcacca ggtatttgcg tttttcgcca
     1621 tcgcgacttt cgatttcgat ttcgcggtta ccacgtttga tttttccgaa ggcaacaact
     1681 ccatcgatct ctgcaacaac tgcagggttt gatgggttac gtgcttcaaa caattcagtt
     1741 acacgtggaa gaccacccgt gatatctcgt acttttccga gtgcacgtgg gatcttggca
     1801 attgtttgac ctgatttcac ttcttcacct tcttcaacgg cgatgtgcgc acctacagga
     1861 aggttatatc ctttaacctc atttcctttt ttatccttaa tgataagtgt aggaattttg
     1921 gttttatcgc gagactcaga aatcactttt tcgcggtgac ctgtttgctc atcactttct
     1981 tcgcggtagg tgataccttc aatgatatgc tcgtaagaaa caaatccggc gaattcagaa
     2041 atgataacag cgttgaatgg atcccaagaa cacattacat cacctttctt catttcatca
     2101 ccatctgaaa ctgaaagtat tgaaccgtaa ggaatattgt tagagatcaa catcttacct
     2161 gttttaggtt caatgatttt aatctcacca gtacgaccaa ttacaacgcg gattttcttg
     2221 ccttcttcat ttttagtttc aacaaaacgc aaaccgtcga attcagcttt accgtcgaat
     2281 ttcgcaacga gtttagattc tgtatcaatg cttccggcaa caccacccac gtggaatgta
     2341 cgaagtgtca actgagtacc cggctcacca attgattgcg cagcgatgat accaacggca
     2401 tcaccttttt gtgcaacacg attattggcg aggttagtac cgtaacattt tacgcacaca
     2461 ccttttttcg attcacaggt caacaccgaa cgaatttcta caaactcaat tgcagtctca
     2521 tcgatacgcg ctgctatgcg atcagtaatt aattcaccgg cagcaacaat caactcgtcg
     2581 gtcagcggat caaatacatt atgcaagctg gtacgaccaa tgatacgttc gctcaatggt
     2641 tcaacgatat cttcattgtc tttcaatgcc gcaatagaaa taccacgcaa tgttccgcag
     2701 tcttcttcag taattaccac atcttgagca acgtcaacaa gacgacgagt aaggtaaccg
     2761 gcatcggcag ttttaagggc cgtatcggca agacctttac gcgcaccgtg agtagagatg
     2821 aagtattcca atacgctcaa tcctgatttc aggtttgaaa ggattgggtt ttcgatgata
     2881 ccaccttctc ctgatgcacc tgatttacga ggcttggcca tcagaccacg catcgctcca
     2941 agctgacgaa tctgttcttt cgaacccctc gcgcctgagt caagcatcat gaacacggaa
     3001 ttgaatcctt ttttatctga tgtcaaacgg ttgatcaaat caccggtaag acgtgtattc
     3061 aaacgcgacc agatatcgat gatctggttg taacgttcgt tttgtgtgat cacaccatac
     3121 atgtggtttg agatcacttc gtctacctct gattgcgccg cagcaacaag gtcagattta
     3181 ttttcaacct ctacaaggtc agaaatatta aatgataatc caccgcggaa tgcagaacgg
     3241 aaaccgagat ctttaatatc gtcaaggaat ttcgctgtac gtggtacatc tgttttgcga
     3301 attacttcac cgataacttt cgcgatcgct tttttcgaca acaacgcatt gataaatcct
     3361 acttcagcag gaacaacttc gttgaacatc acgcgaccaa ctgtagtctc aatcacctta
     3421 gaaacgatca catcattttc cagaacgttc accttacatt taatatatgc atgcaggtca
     3481 acttttcctt cgttgtatgc aataatcacc tcttcggcag agtagaaagc ttttccttcg
     3541 ccttttacaa catcatccgc tgtgctcttt ttaccttttg tggtataata caatccaagc
     3601 accatgtcct gtgaaggaac tgtgattgga tttccgtttt gcggattgag gatattgtgc
     3661 gcagataaca tcaacaattg cgcttccaga attgcagcat tgctcaaagg cacgtgcacc
     3721 gccatctggt caccgtcaaa gtcggcgttg aacgcagaac atactaaggg gtgcaattgg
     3781 attgctttac cctcgatcaa ttttggctgg aatgcctgga tagaaagacg gtgcagtgtt
     3841 ggtgcacggt ttaacatcac aggatgtcct ttcaaaatat tttcgagaat atcccaaata
     3901 acaggctctt tgcgatcaac gagtttcttc gctgatttaa ctgtttttac aattcctctt
     3961 tcaatgagtt tgcgaataat aaatggcttg aataattcag cagccatatc tttcggcaaa
     4021 ccacattcat gtaatttcaa ctcaggacca acaacgataa ctgaacgacc ggaatagtcg
     4081 acacgtttac cgaggaggtt ctgacggaaa cgaccttgtt ttcctttcag cacatcgctc
     4141 aatgatttca atgcacgacc accttctgct ttaacggcat ttgatttacg tgagttgtcg
     4201 aacaatgagt caacagcttc ctgcaacatg cgtttctcgt tacgcaagat tacctcaggt
     4261 gctttgatct cgatcaatcg cttcaaacga ttgttacgga taatcacacg acgataaaga
     4321 tcgttgaggt cggaacttgc aaaacgtccg ccatccaatg gcaccagtgg acgcagttca
     4381 ggtggtataa caggtaaata ttgaactatc atccattccg gacgattttc ataacggcca
     4441 gatgcttcgc ggaacatttc aaccacactc aaacgtttca aagcttctgc tttacgttgt
     4501 tgtgaagttt ctgttcccgc ttgatgacgc aatgccgctg aaagatcatc gagttggatg
     4561 cgcgacaaca tggtgaacac ggcaggtgct cccatgtcgg caataaattt attcggatca
     4621 ttgtcgtcga gcatttgatt ctcgcgaggc aatgcttcca aaatatcgag gtattcctct
     4681 tcagtaatca gatcgtgagt attgtagctc tttaattcgc cattgatcat cacgccttct
     4741 gtcagcggtc cgggttggat cacgcagtaa cgttcgtaat aaacaattga ctcaagtttt
     4801 ttagagctca atcctaacaa atatccgatt ttgtttggaa gagatttgaa ataccaaata
     4861 tgaactacag gaaccacaag tttgatgtga cccatgcgtt cgcgacgtac ttttttctcc
     4921 gttacttcca caccgcagcg atcgcaaacg atgcctttgt aacgaatacg tttgtattta
     4981 ccgcaatagc attcataatc cttgatagga ccaaaaatgc gttcgcagaa taaaccgtcg
     5041 cgctcaggtt tgtatgtcct gtagttgatg gtttcaggtt tcaatacttc accataagaa
     5101 cgctccagga tagagtcagg tgacgccaat ccaatggtga tctttgtgaa gtctgatttg
     5161 atcttgatat cctttttaaa aggcatatgc tgattctgtt ttttcgatga agtaaaactt
     5221 attcgaattt aaggtcgagt gcgagaccac gtaattcatg caccaatacg ttgaacgatt
     5281 caggtatttg cggtgttggc agattctcac cttttacgat ggcttcataa gtcttcgcac
     5341 gacctataat atcatctgat ttcaccgtta ataattcctg cagaatgttt gatgcaccgt
     5401 atgcttcgag tgcccaaacc tccatctcac caaatcgctg accaccaaac tgtgctttac
     5461 cgcccagagg ttgttgcgtg atgagcgagt aaggtccgat agaacgtgcg tgcatcttat
     5521 catcaaccat gtggttcaat ttgatcatat agatcacacc aacagttgct ttctgatgga
     5581 aacgttcacc ggtttcgcca tcgaacaaat aggtacttcc caattttggt aattctgctt
     5641 tcagaatctg ctcgtcgatt tcagcaagtg ttgccccgtc gaaaattggt gtagcgaatt
     5701 tcactcccaa tttcaaacct gcccaaccca atactgtttc ataaatctgt ccgaggttca
     5761 tacgtgacgg cacaccaagt gggttcaata cgatgtctac aggagttcca tcctcaagga
     5821 atggcatgtc ttcctgacga acaattcgtg caacgatacc cttgttaccg tgacgacctg
     5881 ctaatttatc tcccacacgt aatttacgtt tgttggcgat aaatacttta gcaagtttca
     5941 acacaccggt tggcaattca tcaccaacgc tgatattgaa tttctcacgc ttatagcgac
     6001 caagttcttc gtttactttg ataccataat tatgcaggat catgcgcact ttatcatcag
     6061 tatctgct
//
LOCUS       DAOQHY010000186         5907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_7434_length_5907_cov_4.852871, whole genome shotgun sequence.
ACCESSION   DAOQHY010000186 DAOQHY010000000
VERSION     DAOQHY010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7434_length_5907_cov_4.852871"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PLL28_12500"
     CDS             complement(<1..180)
                     /locus_tag="PLL28_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70190.1"
                     /translation="MSDPRDIRILLHASSNDAELLEIADKVLTGERISPAEGIVLYER
                     ASLGFAGTLADHVRTR"
     gene            complement(273..1568)
                     /locus_tag="PLL28_12505"
     CDS             complement(273..1568)
                     /locus_tag="PLL28_12505"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HNF70191.1"
                     /translation="MKKALLAGVLLASTSLMFGQAVDTRCGVHSYLNDLQTYFPEEYA
                     GIKAEAEKRQTVTTTYRTDGGVYTIPVVVHIVYSTDAQNIDDEFVYSQLDVLNADYRH
                     LNADAIETPAAFADLAADVMIEFCLAQQDPDGVITTGITRTETDISSWNLFVSPGAEN
                     YADNVKSSVKGGEDPWPRTDYLNIWVCNLTGGVLGYAMPPGGPSGKDGVVIGYKYFGN
                     DSPGGVYDKGRTATHEVGHWLGMAHIWGDDDFDPEPQCNGSDDMADTPNQEDATYGAP
                     SFPLLDDCAPASPGIMFMNYMDYVDDGSMNMFTEDQAAEMRNIMETTRESILSSQGCV
                     PGVDGINDVLKGQLIQLSVYPNPSSDIFVFEMKNFKNADMTVEVYNFSGELVDRVQLI
                     NNNGTATATFDASMLAAGTYMVKASDGDYILTQQITVVK"
     gene            complement(1764..3053)
                     /locus_tag="PLL28_12510"
     CDS             complement(1764..3053)
                     /locus_tag="PLL28_12510"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HNF70192.1"
                     /translation="METFNKLREHHQPKTTIFIPMHRLFLFLILLISTSMHAQWCGTE
                     TIMDQIRIQQPETWARMQQIRNEKAQGLRLDGEVYTIPVVVHIVYNNEVENIDDLRVY
                     TQINILNEDFRRLNADTIDTPAPFQGVAADVELEFCLASIDPNGDTTTGIIRTYSDTT
                     EWLLTFSENVKYTEYGGDDAWPASSYLNIWVCDLQHGILGYAVGPGGAAEIDGIVVDY
                     SNFGLSDIGSAPYNLGRTATHEIGHWLGLTHIWGDDGNTCDGDDGIEDTPLQLGPTYG
                     CPDFPLTDACSPDYPGIMFMNYMDYTDDACMNMFTMGQKAKMLGVISTYRASILTSAA
                     GCNDIGPLPGDLAELLVYPVPSNGQFTVSVKNFAGTLDMMELHIYNTLGQHIASATLD
                     ATNNTAQYFDLSHLAPGTYVVQAFNGSYFINKAFIIN"
     gene            complement(3040..4110)
                     /locus_tag="PLL28_12515"
     CDS             complement(3040..4110)
                     /locus_tag="PLL28_12515"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNF70193.1"
                     /translation="MAEQDDELELESIAQEGEEELYEHYRVTTDRGQEPLRIDKFLFN
                     RLINISRNRIQNAADAGCILVHGKPVKSSYKIKPFDEISVVLPYKPMKFDLKPEAIPL
                     TILFEDEDLLVVNKPPGLVVHPGLGNYSGTLVNALLYHFQGLPAPKDEPFRPGLVHRI
                     DKFTSGILVVAKQEYAMTHLAKQFFDHSIQRKYIALVWGEFDEPQGTIIGNIARHERF
                     RKMFAVYPPGGEIGKHAITHYRVLESFGYTSLIECQLETGRTHQIRVHMQHIGHPIFN
                     DDTYGGNRIVKGTVYTKYKQFVDNCFDLIPRQALHARSLGFVHPRSGEHLYFETPMPE
                     DMQAAVDKWRAYAQQLKQNGNV"
     gene            complement(4110..4811)
                     /gene="lipB"
                     /locus_tag="PLL28_12520"
     CDS             complement(4110..4811)
                     /gene="lipB"
                     /locus_tag="PLL28_12520"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNF70194.1"
                     /translation="MMQLVHFRDLGTMDYATAWQLQEGVFRDIIDKKLQNRLLPIEKQ
                     LSYQHQLLFVEHPPVITLGKNADDNNVLFNEEALAQRGVQLFHINRGGDVTYHGPGQL
                     VGYPILDLDMFTTDLGVYLRNIEEVIIRTIADYGLVGERSPGETGVWLDVGKPTARKI
                     CAIGIRTSRWVTMHGFAFNVNANLDHFNFIIPCGIPDKAVTSLQNELNSMMSMDEVKQ
                     KVRHYFEIVFDCKLV"
     gene            complement(4811..5791)
                     /locus_tag="PLL28_12525"
     CDS             complement(4811..5791)
                     /locus_tag="PLL28_12525"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HNF70195.1"
                     /translation="MDKYIQQFPEQLVDALRIVDSTATFQLPRTPKHVITSGMGGSGI
                     GSVIVRTLISQQLTVPFAFSSDYRLPGWTNSDTLVLCSSYSGNTEETLSAMQFALDRG
                     CMVVCVTSGGKMLEIAKEKNLPVVIIPGGRPPRSSIGYSIIAQLFLLQHCGIIDNSWH
                     ASMEKCIAFIHENMSVIREQAESLAVEIDGSIPVIYSDALLAGTAVRWKQQLNENAKI
                     HAFTHVVPEMNHNELVAYYHPDHRINVICLRHAPENPRTAKRFSLMEELIQGKAGSII
                     TVLATGPTLFEQIFGLIHLGDWLSVALAERKGVNPLDIEMLEWLKKALDK"
BASE COUNT         1667 a         1308 c         1249 g         1683 t
ORIGIN      
        1 acgtgtgcgc acatggtctg ccagagtgcc cgcgaaaccg agtgaagctc tttcatacaa
       61 tacaatgccc tcagccggcg aaatgcgttc gccagtcaat actttatctg caatttccag
      121 caattcggca tcattggaag aggcgtgcaa taaaatccgg atatcacgtg gatcgctcat
      181 ggaacaaagt tgattgttta cgttggatac aacaaaaaaa gtggctgaaa gatcaaccac
      241 tttttttgaa agataagttc atttaataaa tattatttca caactgttat ctgttgtgtc
      301 agaatataat caccatcgct tgctttcacc atgtaagtgc cggcagcgag catagacgca
      361 tcgaaagtag cggttgctgt tccgttattg tttattaatt gtacacggtc aactaattcg
      421 ccgctgaaat tatatacttc aacagtcata tctgcatttt tgaaattttt catttcaaat
      481 acaaaaatat cgctggaagg gttaggataa acacttaatt ggatcaattg gcctttcaat
      541 acatcattga ttccatcaac accgggcaca caaccttgcg atgaaagaat agattcgcgc
      601 gtagtttcca tgatattgcg catctctgct gcctgatctt ctgtaaacat attcatagaa
      661 ccatcatcaa cataatccat gtagttcata aacataatgc cgggagaagc aggagcacaa
      721 tcatcgagca atgggaaact tggtgcacca taagtggcat cttcctggtt tggcgtatca
      781 gccatatcat ccgagccgtt acattgtggt tccggatcga agtcatcatc accccaaata
      841 tgcgccattc ctaaccagtg gccaacctcg tgtgttgcag tacgaccttt gtcgtaaacg
      901 ccacccggac tgtcattgcc aaaatatttg tatcctatca ccacaccatc ttttcctgaa
      961 ggtcctcccg gaggcattgc atatcctaat accccaccgg tgagattaca aacccaaata
     1021 tttaaataat cggttcgtgg ccatggatct tcaccacctt taactgaact ttttacgtta
     1081 tcagcataat tttcagctcc gggagaaaca aataaattcc atgatgatat gtctgtttct
     1141 gtgcgggtga taccggtagt gattacacca tcaggatcct gttgtgcaag acaaaattca
     1201 atcatcacgt cagctgccaa atcggcaaaa gccgccggag tttcaatagc atcagcattt
     1261 aaatgacgat agtcggcatt caacacatct aattgtgaat acacaaattc atcatcaata
     1321 ttctgtgcat cggtagaata tacaatatgc actactaccg ggatggtata aacaccgcca
     1381 tctgttctgt aagtagtggt aacggtttga cgcttttcag cttctgcttt aattccggcg
     1441 tattcttccg gaaaataagt ttgaaggtca ttcaggtaac tgtgaacacc gcaacgtgtg
     1501 tcaacggcct gaccaaacat taaacttgtg cttgccagga ggacacctgc gagtagagct
     1561 tttttcattt gtttgtcgtg gattggttac tgattagata acaaagttaa caagatgaac
     1621 gcatattgtt aaaagtaact tgttcaatac tgtcaacact ccgccatttc tgcaccttaa
     1681 acttcaaaat cggcataccg tttgcatgta aagtagcagg actcgattct ccgagacccg
     1741 ttttcagctg tacaaaattg taattaattg ataatgaacg ctttatttat gaaatacgag
     1801 ccattaaagg cttgtaccac ataggttccg ggagccaggt gcgagaggtc gaaatactgt
     1861 gccgtattgt ttgtagcatc gagagtggca gaagctatat gttgcccaag tgtgttgtag
     1921 atatggagtt ccatcatatc gagggtaccg gcgaagttct ttacactcac tgtaaactga
     1981 ccgtttgaag gcacaggata aaccaggagt tctgccagat ctccgggtaa tggaccgata
     2041 tcattacaac cggcagcgga tgtaagaatg gatgctctgt aagtggagat aacaccaagc
     2101 attttagctt tctgacccat ggtaaacata ttcatacaag catcatcggt ataatccata
     2161 taattcatga acatgatgcc gggataatcc ggtgaacaag catccgtaag tggaaaatcc
     2221 ggacatccgt aagtagggcc aagttgaagc ggcgtgtctt caatgccatc atcgccatcg
     2281 caagtattgc catcatcacc ccaaatatgt gtaagtccga gccaatggcc gatctcatgt
     2341 gtcgcagtcc ggccgaggtt gtacggtgcg gaaccgatat ctgatagtcc gaagttggaa
     2401 taatcgacca cgataccatc aatttcagca gcgccgccgg gaccgacagc atagcccaaa
     2461 ataccgtgtt gaagatcgca gacccagata ttcaagtatg aggaagccgg ccatgcatca
     2521 tcaccgccat attccgtgta ttttacattt tccgaaaagg ttaacagcca ctcagtagta
     2581 tcggaatagg tgcgaatgat accggttgtg gtatcgccat ttggatcaat tgatgccaga
     2641 caaaattcca attcaacatc agcggctact ccctgaaatg gtgcaggtgt atcgatggta
     2701 tcggcattta atcgtctgaa atcttcattc aatatattta tttgcgtgta aacccgcagg
     2761 tcgtcgatat tttctacttc attattgtaa acaatatgca ctacaacggg aatagtatac
     2821 acttcaccat cgagacgaag tccttgcgct ttttcattcc tgatctgctg catgcgtgcc
     2881 caggtttccg gttgctgtat gcgaatctga tccatgatgg tttctgtgcc acaccattgg
     2941 gcatgcatgg atgtagaaat caataaaatc aaaaatagaa acagacggtg catgggaata
     3001 aagatagtcg ttttaggttg atggtgttca cggagcttat taaacgtttc cattctgctt
     3061 taattgttgc gcataggcac gccatttatc tactgctgcc tgcatatctt caggcattgg
     3121 tgtttcgaaa tacaaatgtt ctcctgatcg cggatgcaca aagcccagcg aacgtgcgtg
     3181 taatgcttgt cgcggaataa ggtcgaaaca attatcgaca aactgcttgt atttggtata
     3241 cacagtacct tttacaatgc ggttaccacc ataagtatcg tcgttgaaaa tcggatgacc
     3301 gatatgttgc atgtgcacac ggatttgatg cgtgcgaccg gtttcaagct ggcattcgat
     3361 taatgaagta taaccaaatg attccaatac gcgataatgt gtgatggcat gttttccaat
     3421 ctcgcctccg ggtggatata ctgcaaacat ttttctgaag cgttcatgac gtgcaatatt
     3481 tcctattatg gttccttgcg gttcatcaaa ttctccccac accaaagcaa tatattttcg
     3541 ttgtatgctg tggtcgaaaa attgtttagc aagatgcgtc atagcatatt cctgtttcgc
     3601 cactacaagt atgcctgatg taaatttatc aatgcggtgc actaatcccg gacgaaaagg
     3661 ttcatccttt ggtgcaggca atccttgaaa atgatacaac aatgcattga ccaaagtgcc
     3721 ggaataattt ccaagacccg gatgcacaac aagaccggga ggtttattca ccaccagtaa
     3781 atcttcatcc tcaaataata tggtaagtgg aattgcctcg ggcttgagat cgaatttcat
     3841 cggcttgtac ggaagcacta ccgatatttc atcaaagggt tttattttat aactggactt
     3901 aaccggtttt ccatgcacga gaatacatcc tgcatcagcg gcattttgaa tgcggttacg
     3961 ggagatgtta atgagccggt tgaataaaaa tttatcaatg cgcagaggct cctgtccacg
     4021 atcggtggtt acacgataat gttcgtataa ctcctcctcg ccttcctgag cgatgctttc
     4081 taattcgagt tcgtcgtcct gctctgccat cacactaatt tacaatcaaa aacgatttca
     4141 aaataatgac gaactttttg cttcacctca tccattgaca tcatgctatt taattcattt
     4201 tgcagtgatg ttaccgcttt gtcgggaata ccacagggga tgatgaaatt aaaatgatcg
     4261 aggttggcgt tcacattaaa agcaaatcca tgcatggtta cccatctgct cgttctgatg
     4321 ccgatagcgc aaatttttct ggctgtaggt tttcctacgt ccagccatac gccggtttct
     4381 ccgggactgc gttcaccaac taatccgtaa tctgcaatgg tgcgaatgat cacttcttca
     4441 atattgcgta aatacacgcc aagatcagtt gtaaacatat ccaggtcgag gatgggataa
     4501 cctactaatt gtccgggacc atgatacgtt acatcgccac cgcgatttat atggaataat
     4561 tgaacaccgc gttgtgcgag cgcttcttcg ttaaataata cattattatc atcggcattt
     4621 ttccccaatg taataacagg tggatgttca acaaataata attgatgttg atacgataat
     4681 tgtttttcta tcggaagtaa cctgttttgt aatttcttat caatgatatc acgaaaaaca
     4741 ccttcctgca attgccacgc tgttgcataa tccatggtac cgagatcgcg aaaatgtaca
     4801 agctgcatca ttatttatcc aatgcctttt tcagccattc caacatttca atatctaaag
     4861 ggttcactcc tttgcgttca gcgagtgcaa ccgacagcca atcgccgaga tgtataagtc
     4921 cgaatatttg ttcaaataat gttggacctg tggcaagcac ggtgataata gaacccgctt
     4981 ttccctgaat gagttcttcc atcagtgaaa atcgctttgc ggtgcgtgga ttttcaggag
     5041 catgacgcag gcaaatcaca tttatgcgat gatcgggatg ataataggca acgagttcat
     5101 tgtgattcat ttccggcact acatgggtaa atgcatggat ctttgcattt tcattgagtt
     5161 gctgtttcca acgcacagct gtgcctgcca ataatgcatc gctgtaaatt accggaatac
     5221 tgccatcgat ctccactgcc agtgattcag cttgctcgcg aataacagac atgttttcat
     5281 gtatgaatgc gatacatttt tccattgacg catgccagct attatcaatt attccgcagt
     5341 gttgcaacag aaataattgc gcgatgatgc tgtagcctat cgacgagcgc ggcggtcgac
     5401 caccgggaat gattaccacc gggagatttt tctccttagc aatttcaagc atttttcctc
     5461 ctgaagtaac acatacaacc atacagccac gatcgagggc aaattgcatg gcgctgaggg
     5521 tttcttcggt attgccggaa tagctgctac agagcacgag ggtatcgctg tttgtccagc
     5581 ccgggagcct gtaatcggag ctgaaagcga acggcacagt aagctgttgc gaaataagcg
     5641 ttcgcacaat taccgagcct atacccgaac cacccatacc ggaggtaatc acatgttttg
     5701 gggttcttgg taattgaaaa gtagccgtgc tgtctacaat acgcaaggca tctaccagtt
     5761 gttccggaaa ttgctggata tatttgtcca taataagccg acaaaaatac ggtattatga
     5821 gatgggagca gtcgctggga aaggaaggtg aagatagagc cgccgtttta ttgcaaaata
     5881 agggttatac cattttgcgc aggaatt
//
LOCUS       DAOQHY010000187         5899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_7450_length_5899_cov_6.890486, whole genome shotgun sequence.
ACCESSION   DAOQHY010000187 DAOQHY010000000
VERSION     DAOQHY010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7450_length_5899_cov_6.890486"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..538)
                     /locus_tag="PLL28_12530"
     CDS             complement(158..538)
                     /locus_tag="PLL28_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70196.1"
                     /translation="MNILSKFTFTLLLLFLHFAVHCQTRQDTLNKIQEYYNFALGKTV
                     AELLSKYNLDTTSNFEADEKLVYFSFDLGFYCCPRIACKNNNYGYWKQLLNQPIVYVE
                     TYNENGILKKIKTKGIRKKDYLPE"
     gene            complement(701..880)
                     /locus_tag="PLL28_12535"
     CDS             complement(701..880)
                     /locus_tag="PLL28_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70197.1"
                     /translation="MGTTGADEYMIAATFTLTGMPNINNVHFDFEPGDHASPGTYNRK
                     YYLDCIRENKEINKQ"
     gene            complement(1001..1300)
                     /locus_tag="PLL28_12540"
     CDS             complement(1001..1300)
                     /locus_tag="PLL28_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70198.1"
                     /translation="MSVPLQEDRFALRNPLLRQAPKRYQILLFACGRHVNSETDMLKS
                     DEHAGVSKDLKNQPDSLSLVQTNQGNKGDNISIWRYDYMADTIIQLRKIKIDTLT"
     gene            complement(1381..2301)
                     /locus_tag="PLL28_12545"
     CDS             complement(1381..2301)
                     /locus_tag="PLL28_12545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70199.1"
                     /translation="MKNKFTFPAYAAMATSFLTINAKVEAKAVYTDIDPDSILTIHEG
                     MEIDFNEDGVWDVFFMLSSWDGTTTYSMHFQAHDIHGGGNWPYGYLGKFSYFYAGSFL
                     GSQALLLPFIAGEEINDSFLFAGGELMYHFSQLSSPFIEWSGGYWYPEFLDGFIGVKL
                     KYFEGGEFKTHYGWIRCSVLDGGNELVIKDYAYELLPDVPIIAGDTVGRIVEFAHDTL
                     PHFGTGLPDADNEIVNASIYSFGSVVYISVPEFLENISLNIYNITGEIVYNRTMNSGF
                     LTVEMTQPSGIYLVELIADIQRTCRKIVID"
     gene            complement(2372..3262)
                     /locus_tag="PLL28_12550"
     CDS             complement(2372..3262)
                     /locus_tag="PLL28_12550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70200.1"
                     /translation="MANKFSLLQYSTAAAALLGVTAASSQVVYTDIDPDVVLDEPNEM
                     FGIDLDDDGLNDFNFFNESFITTVFYGDLANVKALFVGAFDTILNGIAGSTGFLSGGG
                     GYTYYWPYAMQNGVIIDENLNFFNSNYQSLVALKYKIDVPFVLPQGNWTSFYSGYEHL
                     NEYVAIRFADEEAIMRYGWIRCSVVDSGRTLIIHDYAYELQPDQPIIAGDTVSFVNIQ
                     EQPSLFATVYNYNTTIYINLHNYTPTQLTIVNLTGQTILDKSIYNQFEIVDMNYFPKG
                     IYLVTLKEGENILTRKVAIE"
     gene            complement(3265..5733)
                     /locus_tag="PLL28_12555"
     CDS             complement(3265..5733)
                     /locus_tag="PLL28_12555"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70201.1"
                     /translation="MKNLIIKQRMLVLSIYIISFVFCNNKIYCQDIDTLEWEEIVDLA
                     DTVYNSGMDTIYVIEDSTSETTGFRTGASLDCATPASTMSINLNKTVKTINKGQFGVN
                     VTGMFTKSTLYGDIYSEDQWQWLSDMRPKVLRFPGGSSSKFMHLLTGDKGYGYDIVEI
                     TRYLDAIDNDLEAPTNATDIMNETNVTTLLNWFGGSSEILDAFEEYRTDYGYQEDLTS
                     GDRFIDHFIRLIQKIETDNQVFDPTYKVDVIVCLNILTETAEDCLDIVQYLQSNPIHN
                     VNVVGVEMGNETANKFHKQIMQFDEFENYWQYLDGQAVVGQASKEAAIGESLFIPAAN
                     RNFFYVFRNRSGVNYKIGLCAEGLNGTGEVFNILPAEDAGSRSVDWNVALRSHYGDTH
                     AGTSVKKFHAVILHTYFSADSWYNECVTETGILGSYSCPEWDFDNADTRIQPAFDAAR
                     LNFRKFMLTRFDNDLDLFNLDLNFNLTSSIKKDMWITEWNLKDEGTPNNGKIFTNSYQ
                     HTVLLQEWWLKNLKLNFTEGYRENFFKYATLQNYAGGSGVQMLTPADEDVELTLLGQD
                     VSPYNLAAGDPAKRNYYVKRTVGYTMDLISEINKNNLNYFPATVATYGHNPNLPPTFF
                     ITPAKDFVYMYFTNSRCNDQRYVLNPAGMYLMYGYQVELVDAEMFVVDAFQAYSQSGK
                     SMIYDFNSCYTPANPYDMEIDQTYNYVNPVCTAADGSLCITVPGFTSGYVKMAVVPYV
                     PRMADEINTSLSIYPNPANNLINISSTEQIVSVAVISMTGAKMFETIDNCNIIDVSML
                     SYGMYQVICTFSDGSNVTKSFIKL"
BASE COUNT         1829 a         1160 c          993 g         1917 t
ORIGIN      
        1 ttgttttacg gctaaaaccg cgcgggttta aaatgaacct atgcgtctac ctataatgta
       61 aaataaaaag tccaaaagta atttgagact tggcagcata actttcttcc ttgtaaacag
      121 aaagacgtca gcttgttagc catcttgatt taggctacta ttcaggtagg tagtcttttt
      181 ttcttatacc tttcgttttg atttttttaa gaattccgtt ttcattgtac gtttctacgt
      241 atacaatagg ttgattcagt aattgtttcc aatatccgta attgttgttt ttacaagcaa
      301 tgcggggaca acaataaaat cctaagtcaa atgaaaagta tacaagcttt tcatctgctt
      361 caaaattact tgtagtgtcc aagttgtatt ttgagagaag ctctgcaact gttttcccaa
      421 gagcaaagtt gtaatattct tgaattttat tcaaagtgtc ttgtctggtt tggcagtgaa
      481 ctgcaaaatg caaaaatagt agcaggagag tgaaagtaaa cttagataaa atgttcattg
      541 tgtctcactt taaataacct ctaacggtcc gtggctttgc gcagcgcggg agtttcgccc
      601 aaacaaatgt atcaattgaa tcttgaattt acgagcaacc ttgctcttat tggattcctc
      661 gaatgcccgc gttaagaaaa actgcggtta catgttggat ttattgttta tttatttctt
      721 tgttttctct aatacaatcc aaataatact ttctgttgta tgttccggga gatgcatgat
      781 ctccaggttc aaaatcaaaa tgcacattgt tgatatttgg catcccggtc aacgtaaatg
      841 tagcggctat catatattca tcagcaccag tcgtccccat ttgttgtgtt aaatattcac
      901 tattatcaat cttaacatag ataatgtcct tagaaatttt caggtattca agcttgacct
      961 tatcccgata ttttgcgttt atggtgttaa tcaaattcca ctatgtcaaa gtatctattt
     1021 ttatttttct cagttgaata atcgtatcgg ccatataatc atatctccag atagaaatat
     1081 tgtctccctt gtttccttgg tttgtttgaa caaggcttaa tgagtcaggt tgatttttca
     1141 aatcctttga cacgccggca tgttcatccg atttaagcat gtctgtctct gaatttacat
     1201 gtctaccgca agcaaataga agaatttggt aacgttttgg cgcttggcga agaagcggat
     1261 ttcgaagcgc aaatctgtct tcctgaagtg gaaccgacat attgcattgc atttcgtcaa
     1321 aaacacagat caagcttttt cgctaagcgc ctgttataac cagtggttta ggatcaattc
     1381 ctagtcaatt acaattttcc tgcaagtcct ttgtatgtct gcaattaatt caactaaata
     1441 aattccactc ggttgtgtca tttcaacggt caggaaacca ctattcatgg ttctattgta
     1501 aacaatctcg ccagtaatgt tatagatgtt taaggatata ttttctaaaa attctggaac
     1561 actgatataa acaaccgatc caaatgaata aatacttgca ttaacaatct cattgtctgc
     1621 atctggaaga ccggttccga aatgtggtaa tgtatcgtgg gcaaattcta caatcctacc
     1681 aacagtatcg ccagcgatta ttggcacatc cggcaataat tcataggcgt aatcttttat
     1741 cacaagttca tttcccccgt ccagaacact acagcgaatc caaccgtagt gggttttaaa
     1801 ttcgccgcct tcaaaatatt tcaatttaac tcctatgaat ccatctagaa attctggata
     1861 ccagtatcct ccactccatt caatgaacgg agaggacaat tgggaaaagt gatacatgag
     1921 ttctccacct gcgaacagga aactgtcatt tatttcctca cccgcaataa atggcagcaa
     1981 taaggcttga gagcccaaaa aacttccggc ataaaaatat gaaaatttgc ctaaatatcc
     2041 ataaggccaa ttgcccccac catgaatatc atgggcttga aaatgcatcg aatatgttgt
     2101 agtcccatcc catgacgaaa gcatgaaaaa aacatcccag actccgtctt cattaaaatc
     2161 aatttccatt ccttcatgaa ttgttaagat tgagtctgga tcaatatcag tataaaccgc
     2221 tttagcttct acctttgcat taattgttaa aaatgaagtg gccattgcag catacgcagg
     2281 aaacgtgaat ttattcttca taaattacga ttttataaat gatagctgcc gccattttgc
     2341 aaagcttttg ttataggatg tggtctttcg attattcaat tgctactttc ctagtcaaaa
     2401 tattttcacc ttctttcaaa gtaactaaat aaattccttt ggggaagtaa ttcatgtcta
     2461 caatttcaaa ttgattgtat attgacttat ctaaaatagt ctgcccggta agattaacaa
     2521 ttgttaattg tgttggtgtg taattgtgta aattaatata aatggtagta ttataattat
     2581 aaaccgttgc aaaaagcgaa ggctgttctt ggatatttac aaagcttact gtatcaccgg
     2641 caataatagg ttgatccggt tgcaactcat atgcataatc atgaataatt aaagttcgtc
     2701 cgctatctac caccgaacaa cgaatccatc catagcgcat gatagcctcc tcgtctgcaa
     2761 accgtatagc aacatattca tttaaatgtt cataaccaga ataaaaagaa gtccaatttc
     2821 cttgtggaag aacaaaagga acatctattt tgtacttgag tgctactaat gattgataat
     2881 tactgttaaa gaagttcaaa ttttcatcaa taataactcc attttgcatg gcgtacggcc
     2941 aatagtaggt atacccacca ccgccagata aaaatccagt gctaccagct atcccattta
     3001 agattgtatc aaatgcaccg acaaaaagtg ctttcacatt tgccaagtcc ccataaaaaa
     3061 ccgtagttat aaaactttca ttaaaaaaat taaaatcatt caacccgtca tcatccaagt
     3121 caatgccaaa catttcattc ggttcatcta gcaccacatc ggggtcaata tcggtataaa
     3181 ccacctgtga tgaagcagca gtaaccccaa gaagggctgc tgctgctgtt gaatattgta
     3241 ataaagaaaa tttgtttgcc attattacaa ttttataaaa gattttgtaa cattacttcc
     3301 atcactaaag gtacaaatta cctgatacat gccataactg agcattgaaa catcaattat
     3361 attacaatta tcaatagttt caaacatttt ggctccggtc atcgaaataa ctgctactga
     3421 aacaatttgt tcggtgcttg aaatatttat gaggttgttt gccggattag ggtaaattga
     3481 aagcgaagtg tttatttcat ccgccattct tggaacatat ggcactactg ccatttttac
     3541 atacccggac gtaaatcccg gaacagtaat acacagacta ccatctgcgg cagtgcaaac
     3601 cggattaacg taattatatg tttgatcaat ttccatgtca tacggattgg caggcgtata
     3661 gcacgaattg aaatcataaa tcatgctttt cccactttgc gaatatgcct gaaaagcatc
     3721 tacaacaaac atttcagcat ctaccagctc aacctgataa ccatacatta aatacatccc
     3781 tgcaggattt aatacatacc gctggtcatt gcaccgacta ttagtaaaat acatatagac
     3841 aaaatctttg gcaggggtaa taaaaaatgt tggcggcaaa ttgggattgt gcccataagt
     3901 agctacagtt gccggaaagt aattaagatt attcttgttt atttcgctga ttaaatccat
     3961 cgtataccct acggttcttt ttacatagta attccttttg gccggatcac ctgctgctaa
     4021 gttgtatggt gaaacatcct gtccaagcag tgtaagttca acatcttcgt cagcaggggt
     4081 gagcatttga acaccagagc ctccggcata gttttgtaaa gtagcgtatt tgaagaaatt
     4141 ttctcgataa ccttcagtaa aatttagttt taagtttttt aaccaccact cctgtaacag
     4201 tactgtgtgc tgataactgt ttgtaaatat ttttccgtta tttggcgtgc cttcatcttt
     4261 taaattccat tcagtaatcc acatgtcttt tttgattgaa gaagtgagat tgaagttcaa
     4321 gtcaaggtta aacaaatcaa ggtcattatc gaatcgggtt aacataaatt ttctgaaatt
     4381 taatcttgcc gcatcaaaag ccggttgtat gcgggtatct gcattatcaa aatcccattc
     4441 agggcaagaa tagcttccta aaatacctgt ttcggttaca cattcattat accatgaatc
     4501 ggcagaaaaa taggtgtgca aaataactgc atggaatttt tttacactgg ttccggcatg
     4561 agtgtcacca taatgggagc gcaaggcaac attccaatca actgaccggg aacccgcatc
     4621 ctcagctggt aatatattaa aaacttcacc cgttccatta agtccttcgg cgcataaacc
     4681 aattttatag ttcacaccag acctgtttct aaagacatag aaaaaatttc tattcgcagc
     4741 gggaataaat aaagattcac caattgctgc ttctttactt gcttgaccca ctacagcctg
     4801 accatccaaa tattgccaat aattttcaaa ttcatcaaac tgcataattt gtttgtgaaa
     4861 tttatttgcc gtttcgttac ccatttcaac gccaactaca ttaacattgt gaatagggtt
     4921 tgattgaagg tattgaacaa tatccagaca atcttctgcc gtttctgtca gaatgttcag
     4981 gcatacgatt acatctactt tgtaggttgg gtcaaatacc tgattatctg tttcaatttt
     5041 ttgaattaaa cgaataaaat ggtcgataaa tcggtcgccg gaagttaaat cttcttgata
     5101 tccataatca gttcgatatt cttcaaatgc atctaatatt tctgaacttc cgccaaacca
     5161 gtttaaaagt gttgttacat ttgtttcatt cataatatct gttgcatttg ttggtgcttc
     5221 taaatcatta tcaattgcat ctaaatatcg agtaatttca acgatgtcat aaccatatcc
     5281 tttgtcgccg gtaagcaaat gcatgaactt actgcttgaa ccacccggaa agcgcaatac
     5341 ttttggacgc atgtcgctaa gccactgcca ttggtcttca ctgtaaatat ctccgtataa
     5401 cgttgatttt gtaaacatgc ccgttacgtt aactccgaat tgacctttat ttatggtttt
     5461 tacagtttta tttaagttaa tactcatggt tgaagcaggc gttgcgcaat cgagcgatgc
     5521 acctgttcta aatccggtgg tttctgaggt agaatcttca attacataaa tggtatccat
     5581 tccactattg taaactgtgt cggcaaggtc aacaatttct tcccattcaa gggtatcaat
     5641 atcttggcaa taaattttat tgttacaaaa tacaaatgaa ataatatata ttgataaaac
     5701 caacatgcgc tgttttatga ttaaattttt catgttaata aatttaaggt aaataaaaac
     5761 aagtgaaagt gtgttcctaa gggattattc ataacactac atcgtcgtct acgcttgttc
     5821 agtctgtcaa ccgaccagat tgtgaattgc tcactccgct ttttggaact gcgaacttga
     5881 tagaccatat cctataacg
//
LOCUS       DAOQHY010000188         5859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_7525_length_5859_cov_5.465886, whole genome shotgun sequence.
ACCESSION   DAOQHY010000188 DAOQHY010000000
VERSION     DAOQHY010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5859
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7525_length_5859_cov_5.465886"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..384
                     /locus_tag="PLL28_12560"
     CDS             112..384
                     /locus_tag="PLL28_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70202.1"
                     /translation="MTTNSGEINSKDFADQAVNALSNLKRAMPKDWKKAIQPYQEQIK
                     NQASEASVSTTTAVLMLVKKINASAISMSIVKIQHFFLLAAYCDML"
     gene            complement(690..1052)
                     /locus_tag="PLL28_12565"
     CDS             complement(690..1052)
                     /locus_tag="PLL28_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNF70203.1"
                     /translation="MLTAVCPKIPMRNKIISREFYVGKLGFTELGDPDFMGYLMVKKD
                     DVEIHFFEYRDLDPLQNYGQIYIRTNDIDSWHNLAKEQALMFAKFGQMQAKPWKQKEF
                     SLLDPDHNLLTFGQYIGE"
     gene            complement(1081..2274)
                     /locus_tag="PLL28_12570"
     CDS             complement(1081..2274)
                     /locus_tag="PLL28_12570"
                     /inference="COORDINATES: protein motif:HMM:NF010177.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-2-enoyl-CoA reductase family protein"
                     /protein_id="HNF70204.1"
                     /translation="MIIEPRMRGFICLTAHPEGCAKNVSNQIAYVRSKGAIAGAKKVL
                     VIGASTGFGLASRISAAFGSGAATIGVFFEKAPQEGKTASPGWYNSAAFQAEAEKAGL
                     YAKSINGDAFSNEIKTKTIDTIKADLGKIDLVIYSLASPVRTHPVTGVTHRSVLKPIG
                     STFKNKTVDFHTGIVSEISIEPCNEEDIQNTIAVMGGEDWEMWMDALLKADVLADNAM
                     TIAYSYIGPSLTEPVYRKGTIGRAKDHLESTAGVITNKLQSVHGKAFVSVNKALVTQA
                     SSAIPVIPLYISLLYKVMKENGIHEGCIEQMQRLFADRLYNGSTIPVDEAGRIRIDDW
                     EMRKDVQDTVAALWLEATTETLPAIGDLKGYKSDFLNLFGFGFDGVNYPTDTNEMVNI
                     KGLVD"
     gene            complement(2355..2708)
                     /locus_tag="PLL28_12575"
     CDS             complement(2355..2708)
                     /locus_tag="PLL28_12575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNF70205.1"
                     /translation="MEFKVEKKTGQVIVIHLKGRMIGEYETIPLNEQLEDLIEENFVR
                     IIFDLKELEYMNSSGLNFFLKTLTKVRRLDGEVVICAMNNLLETLMVTTKLSSFFTIC
                     PTLDAALDHFEKEKA"
     gene            complement(2719..3213)
                     /locus_tag="PLL28_12580"
     CDS             complement(2719..3213)
                     /locus_tag="PLL28_12580"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNF70206.1"
                     /translation="MEKEMQQTKYLEEIRNIFAAHLESKGLRKTPERFTILEEIYSRN
                     DHFDAESLYSHLLMKNYHVSRATVYNTLELLLSCDLVTKHQFGKNLAQYEKSYGYKQH
                     DHIICVDCKKVVEFCDPRIHQIQTMMGDLLNFKITHHSLNMYGVCGSCQKNRDRKSGE
                     TENK"
     gene            complement(3223..5430)
                     /locus_tag="PLL28_12585"
     CDS             complement(3223..5430)
                     /locus_tag="PLL28_12585"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNF70207.1"
                     /translation="METTSPIDLQKERKLILKEYRNLLRGLRNNMTAAERKQIRLAFE
                     MAVDAHKEMRRKSGEPYIIHPLRVAQIVVNEMGLGATAAICALLHDVVEDTELTLDDI
                     KREFGIKVMKIIDGLTKVSGVFDLTSSIQAENFKKLLLTLADDVRVILIKIADRLHNM
                     RTMDSMPRKKQLKIASETLYLYAPLAHRLGFYAIKSELEDLGMKYTESEAYKDIAKRL
                     AETKRERSRYMNEFMKPIRERLEKDGFHFEMYGRPKSIYSIYNKIKNKGVSFDEVYDL
                     FAIRVIIDTPFDHEKADCWRVYSTVTELYHPNPDRLRDWISSPKSNGYESLHTTVMGP
                     KGRWVEVQIRTKRMDEIAEKGFAAHWKYKEGSNDKALDTWLREIRELLRNPEFNALEF
                     MDDFRLNLFNKEIYVFTPKGELKIIPQGATALDFAFEIHTAVGYHCIGAKVNHRLVPL
                     SHRLSNGDQVEIITSKKQKPNEDWLNFLVTSRARSKVKNALKEEKRAIAAEGRIILEK
                     KLKSHKAELFDRNLQLLMEFYHQPTSLELCYRIGTKDIALKELKDFGVHQGKLIPPSK
                     DKQKPVDLAKLEKLATSNAELLIFDNALDDIKYSLAKCCNPIVGDDVFGFITINEGIK
                     IHKTSCPNATEMMSKYGYRIVRTRWTQHKDIAFLTGVKVTGLDDVGLVNKVTNIISGE
                     MRLNMQSLLMDAKDGVFEGTIKVFVKNTDELDKLMNKLRTMKGVISVTRLETT"
BASE COUNT         1601 a         1312 c         1129 g         1817 t
ORIGIN      
        1 atttcgatat attatcctgg ctggatagta agatcagaaa tatttctttc acacaggcga
       61 tctacgagaa acacgggcat ttgttggtat aagttgtatc tttaaatcac catgaccacc
      121 aactccggcg aaataaactc taaagacttt gcagatcaag ctgtaaatgc cttgtcgaac
      181 ctgaaacggg caatgcctaa ggactggaaa aaggcaatac aaccttacca ggaacagata
      241 aaaaatcaag cctctgaagc ttctgtaagc actacaacag cagttttaat gcttgtgaaa
      301 aaaatcaatg cttctgcaat atctatgagt attgttaaaa tacagcattt ctttttgttg
      361 gccgcgtatt gtgatatgtt gtgaggtatg gtttgtgagc tgtgatttat ggggtgtgtt
      421 tgtggtacgt attttttcgc gcaaaggcgc taaggggcag aggcgctaag ttgaggtgag
      481 tggtgagtgg gcactacgca aattcgctcg caaagtgagc gaataattgc attagtctca
      541 ctactcaata cccactactc actaccagtc gggaatcgtg attcgtgagt cgtgagtcgt
      601 gaatttttac ctgatacttg atacctgata cttgatacct gatacctgat acctgatacc
      661 aagtctagat acttgcctct agctactatt tactccccga tatactgccc gaaagtgagc
      721 aagttatgat ccgggtcgag gagggaaaat tctttttgct tccagggctt ggcttgcatt
      781 tgtccaaatt tggcaaacat cagtgcctgc tctttcgcca ggttgtgcca ggagtcgata
      841 tcattggtgc gaatgtatat ctgcccgtaa ttctgcaaag gatcaagatc tctgtattca
      901 aaaaagtgga tttctacatc atcttttttc accatcaggt atcccataaa atcaggatca
      961 cctaattctg tgaaaccaag tttgccgaca tagaattcgc gcgaaatgat tttattgcgc
     1021 ataggaattt taggacaaac ggctgtgagc ataacttaat aatgattagt gagttgtaag
     1081 ttaatcaacc aaacccttaa tatttaccat ctcattcgta tccgtcggat aattcacacc
     1141 atcaaatcca aatccgaata aattcaagaa atcagattta taccctttca gatcgccgat
     1201 ggcaggtaat gtttctgttg ttgcttcaag ccataatgcg gctaccgtat cttgcacgtc
     1261 tttgcgcatt tcccagtcat caatgcgtat gcgacccgcc tcatcaacag gtatagtaga
     1321 tccattataa agcctgtcag caaacaatcg ctgcatttgt tcaatacaac cttcatggat
     1381 gccattttct ttcatcacct tgtataacaa tgagatatac aatggtataa ccggaattgc
     1441 agaacttgct tgtgtaacca gtgctttatt tacagagaca aatgcttttc catgtacaga
     1501 ctgcaattta ttcgtaataa caccggcagt agattccaga tgatctttag cgcgaccaat
     1561 agttcctttg cggtatacag gttctgttaa tgacggaccg atatagctat acgcaatggt
     1621 catcgcgtta tcagccaata catctgcttt cagtaatgca tccatccaca tttcccaatc
     1681 ttcaccaccc attacagcta tagtattctg aatatcttct tcattgcatg gttcgatgct
     1741 tatttcagaa acaatacccg tatgaaaatc aacagtctta tttttaaaag tgctgccaat
     1801 aggtttcaac accgaacgat gtgttacacc tgttacagga tgtgtgcgaa ccggagaagc
     1861 aaggctgtaa atcacaagat caatttttcc gagatctgcc ttgatggtat cgattgtttt
     1921 cgtttttatt tcattggaga atgcatcacc gttaatactc tttgcatata atccggcttt
     1981 ctcggcttca gcttgaaacg ccgcgctgtt ataccaaccc ggtgaagcag tctttccttc
     2041 ctgaggtgcc ttttcaaaaa atacaccgat agttgccgcc cctgacccaa aagcagcgga
     2101 aatacgcgat gccaatccaa aaccggtgga tgcaccaatt accaatactt ttttagcacc
     2161 tgctatagca cctttcgaac gtacataagc aatctggttg ctgacatttt ttgcgcaacc
     2221 ttccggatgt gctgtcaaac aaataaatcc acgcattctt ggttctatga tcatactatt
     2281 tggttttaat gattgtttgg gtaatgaatt gcaaagttaa acaattgaca tgacctgaat
     2341 ttccggaatc caattcaagc tttttctttc tcgaaatgat cgagtgccgc atccaatgtc
     2401 gggcagattg taaaaaagga gctcaatttt gtagtaacca ttaaagtttc aagcagatta
     2461 ttcatggcac aaataactac ttctccatca agtcggcgca cttttgtgag tgtttttaaa
     2521 aagaagttca atccgctgga gttcatatat tccaattcct tcaaatcaaa aataatacgc
     2581 acaaagtttt cttcaattaa atcttccagt tgttcattca atggtatagt ttcatactcg
     2641 ccaatcatgc gaccttttaa atgaataaca attacctgcc ctgttttctt ttctacttta
     2701 aattccatat attcaaattc atttattttc cgtttcaccg gatttgcgat cgcggttttt
     2761 ctgacaactg ccgcaaacgc cgtacatatt caaactgtga tgtgttatct taaaatttaa
     2821 aaggtcaccc atcatggtct gtatctgatg gatgcgcgga tcgcaaaatt ccacaacctt
     2881 tttacagtcg acacaaataa tatgatcgtg ttgcttatat ccatacgatt tttcatactg
     2941 tgccagattt tttccgaact gatgtttggt caccagatcg caggaaagta atagttccaa
     3001 tgtgttgtaa actgtagccc tgctcacatg atagtttttc atcagcagat gcgaatacaa
     3061 gctttcagca tcaaaatgat cgttgcgact gtatatttcc tccaatatgg taaatcgctc
     3121 aggtgttttg cgcagacctt ttgattcgag atgcgccgca aaaatgttac gtatctcttc
     3181 aaggtacttt gtttgttgca tttctttttc cattacaatt ctttaagtgg tttcaagacg
     3241 agttacggaa ataactcctt tcattgtgcg caatttattc atgagtttat ctagctcatc
     3301 cgtatttttt acaaacactt taatcgttcc ctcaaacaca ccatccttgg catccatgag
     3361 gagactttgc atattcaaac gcatctcgcc actaatgata ttagttacct tattcaccaa
     3421 accaacatcg tctaatcccg taacttttac accggtgaga aaggcaatat ctttatgttg
     3481 cgtccacctg gtacgaacaa tgcgatatcc atacttactc atcatttcag ttgcattcgg
     3541 acaactggtt ttatgaattt tgataccttc attgatcgtg ataaatccaa aaacatcatc
     3601 gccaacaata ggattacaac atttcgcaag tgaatattta atatcatcga gcgcattgtc
     3661 gaatatcagt aactcggcat tgcttgttgc cagtttttca agcttcgcaa ggtcgacagg
     3721 tttttgtttg tctttcgatg gaggaatgag cttgccctgg tgtacaccaa aatcttttaa
     3781 ctcctttaac gcaatatctt ttgtgccgat gcgatagcat aattcgaggc tggtaggctg
     3841 atgataaaat tccatcagca attgtaaatt tctgtcgaac aattcagcct tgtgactttt
     3901 cagtttcttt tccagaatta ttcttccctc tgcagcgata gcgcgtttct cttctttgag
     3961 ggcatttttc actttgctcc tcgcgcgact ggtaaccaaa aaattcagcc agtcttcgtt
     4021 cggtttttgt tttttggatg tgataatctc tacctgatcg ccattgctca agcgatgact
     4081 cagtggcacc agacgatgat ttactttcgc accaatgcag tgatatccaa cagcggtatg
     4141 aatctcaaaa gcaaagtcga gtgcagtagc accttgagga ataattttca gttcaccttt
     4201 tggcgtgaac acataaattt ctttgttgaa taaattcaac ctgaaatcat ccatgaattc
     4261 cagtgcgttg aattccggat tgcgcaacaa ctcacgtatt tctctcagcc aggtatccag
     4321 cgctttatcg ttagaccctt ctttatattt ccaatgcgcc gcaaatccct tttcagcaat
     4381 ttcatccatg cgttttgtgc gtatttgcac ttccacccac ctacctttcg gtcccatcac
     4441 ggtagtatgc aaactttcgt aaccgttact ttttggcgat gaaatccaat cgcgcagcct
     4501 gtcgggattt gggtgatata attccgtaac tgtagaatac acgcgccagc aatcagcttt
     4561 ttcgtgatcg aaaggcgtat ctatgattac gcgaatagcg aagagatcat acacctcatc
     4621 gaagctgacg cctttatttt tgattttatt gtaaatggaa taaatacttt tcggacggcc
     4681 gtacatttcg aaatgaaaac catctttttc caggcgttcg cgaataggct tcatgaattc
     4741 attcatatat cgtgaacgtt ccctttttgt ttctgccaat cgcttggcta tgtctttgta
     4801 agcttccgat tcggtatatt tcataccgag gtcttcaagc tcacttttga tagcataaaa
     4861 tcctagtcga tgtgcgagtg gcgcatatag atataaagtt tctgaggcaa tcttcaactg
     4921 ttttttgcgc ggcatggaat ccatggtgcg catattgtgc agacggtctg caattttgat
     4981 caggatcacg cgcacatcat cggccagcgt caacagcagt ttcttaaaat tctcggcttg
     5041 aatagagctg gtaaggtcaa acacgcccga caccttcgta agaccatcga tgatcttcat
     5101 tactttgata ccgaattcgc gtttgatatc atcaagggta agttctgtat cctccaccac
     5161 atcatgcaac aaagcgcaga ttgctgcagt agcgccaagg cccatctcat tcaccacgat
     5221 ctgtgccacg cgaagcgggt gtatgatata cggctcacca ctcttgcggc gcatctcctt
     5281 atgcgcatcc acagccatct caaaggcaag cctgatctgc ttccgctccg ccgcggtcat
     5341 attattccgc agtccgcgca gcaaattccg atactcctta agtatcagct tccgttcttt
     5401 ctgaaggtcg atagggctgg ttgtctccat gtacagtcta gactacaaag ctattacata
     5461 actcatttgc ttgtaaatta gttttgcacg caaagaaaag gatggggaca tggggtgcga
     5521 aggagtcagc gtaaatcttt aggactatcc gcagaattcc tatagcccct aggtgggatt
     5581 cgagaacccc tcggggctct actacgctgc gttttacgag gatatgtcat tacgcatcct
     5641 agatccataa actgagattt tccacgggtc atcggacagg acttacaagt gaatcaatca
     5701 caaatcttgg gagtcccgtg tcagaaaaat attcagaggg agaacgtttg gtgggtcggc
     5761 gcgtgctttc atgcctaaca taccgggtgc tcactgaagc attttgagaa tgatgtggcc
     5821 tacattgccg gatggctcaa gaaactgaaa gacgacaga
//
LOCUS       DAOQHY010000189         5806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_7621_length_5806_cov_5.887150, whole genome shotgun sequence.
ACCESSION   DAOQHY010000189 DAOQHY010000000
VERSION     DAOQHY010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7621_length_5806_cov_5.887150"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PLL28_12590"
     CDS             complement(<1..292)
                     /locus_tag="PLL28_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HNF70208.1"
                     /translation="MRSRLVVIGCCLLLSASVAMQTNTFRQASYFPPTSYNFDENPLT
                     DAKVALGRILFYDPILSADSTISCASCHSSYNAFSHTDHKLSHGIRGEIGTRN"
     gene            complement(289..963)
                     /locus_tag="PLL28_12595"
     CDS             complement(289..963)
                     /locus_tag="PLL28_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70209.1"
                     /translation="MIPYLFTLLSLLPAKPEKLSYGTITFNATMQGEPMQLHTMYATG
                     HGNDSVSLSTCKFYIVDIVLLDGSKVVGEPELKYILIDLSDSSSLNIPFVYKGHFNGI
                     RFTLGVDSATNMSGPKAGALDPVNGMYWTWQSGYINAKIEGIRTGCDTAREFLYHIGG
                     YHAPDQTALVTYRICKTSEKINVTLDMERFFSTVTCDMDATVQIPGAKAHALAAYMSA
                     SFVINQ"
     gene            1113..2861
                     /locus_tag="PLL28_12600"
     CDS             1113..2861
                     /locus_tag="PLL28_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70210.1"
                     /translation="MKKGFRHITSVIAFVVIAFASTILQAQTYNIATYNGMTVTTCSG
                     NFYDSGGSGGNYGNNENYVVTFCSASGSPLYFDFTSSSSALNLDAGTGDTLFFYDGLT
                     TSDPLIATLVKADDYAFSYPAVGTFSSCVTIKWKSNASAVDGGWQASISCEEPPTCAD
                     NPPAADVFGQATAVCNLNDYCGTTSRYYGEDAPFNFGGTGGSCPGPDDGLFAGTLENN
                     SWLKFIADATSASFDFNVTGGASCDGVQVGIFSFDAATNTFARKSPCNYTDGGWSGAF
                     TLTATGLTVGQTYYMMIDGNAGSNCDYTIGAGTGVATVSAGADQIVCAFNTTLAATTA
                     MGSGTWSVVSGTGVFADATNPTTYVSGLSYGINVFRWTSTGGLCMDVGAYDDVVVENT
                     CLLPITLSDFTATVVDDMVRLNWTTASEIDNDHFVLERAGADQQFMAIAEIMGSGTTT
                     IPVNYTYTDDMPLPAVNYYRLKQVDVNANTSTSNVIAIESHSDAQTFTIGDCYFDNAS
                     EVLTIPYQTSASVALDIQLCDIAGRILFSATCHTTAVNDNAQVDLHHLTQTGLILLQI
                     TGDNGVRSNHTFWISE"
     gene            complement(2998..5349)
                     /locus_tag="PLL28_12605"
     CDS             complement(2998..5349)
                     /locus_tag="PLL28_12605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HNF70211.1"
                     /translation="MRSLKHILLFTYLSMYVVGAFSQGLEKIWGKSIGGSDNDYLKQI
                     KQTRDGGCVICGYSYSDDFDIPENKGSTDFLLIKMNSSGDIEWIQTYGGSSIDRCYGV
                     DTTLDGGYIMAGYTYSNDFDVTDNLEVSAWVVRVDSIGNIVWQKTYGGSGFDAADAGI
                     ISLDDGSFVIAGQSYSNDGDVIGHVGTTDYKDFWVFKIDAVGDIIWSNSYGSTKHDHA
                     YGISKADSGFIVTGVCGGGDGDVTYFKGGFSDFWTIYINDTGELIWQSAQGGSDSDEG
                     VSCAAFGDTAYTTGIILSDDGDVTDHIGDWDAWVTKTYIGSLINAKSFGGHEGDGLFG
                     CTEFQINKIATCGYSESFDGDLTANLGSADFWIAIIDSSLQITSQISLGGSESDKAYS
                     IINTPDNCLLVGGVSNSDDYDVDSNYGLSDIWIVKLAICDNHYFADSDGDGYGNLLSD
                     TLACSAPVGYVADSTDCNDTDASQYPSAIDICNAFDDNCNGLIDEDATFNVYYLDFDG
                     DNYGDVSIDSTSCITPPGYVNNSLDCNDADLYINPDAPESCNGIDDNCNALIDDGLIV
                     YTFYIDADGDTFGNPLIVIDTCVEAVPGYVTNDLDCNDLLASIYPGTTEVCNALDDDC
                     NGIIDDNLTFTWLYQDADGDLFGNANIDTLTCMEISGYVLDSTDCNDTNPAVFPGAIE
                     LLNGIDDDCDFLADEGLVVQNGSFPNIEIFPNPTNNIFNIGLPSGTFVTISISDINGA
                     LIFDQVECSGNTNIFDLTDQPAGIYWVKIICKGWIRMLSVEKI"
     gene            5499..>5806
                     /locus_tag="PLL28_12610"
     CDS             5499..>5806
                     /locus_tag="PLL28_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70212.1"
                     /translation="MQLSPAWSSLPLKTNNSTLTEKSSTCLPLAFDESKFPNNFSWLV
                     FPDSTLSDSIVYAEGDTVEIYTSGPDSIVVIFESTAPDSLLEDAWTISPGDPRMLIHV
                     T"
BASE COUNT         1700 a         1307 c         1123 g         1676 t
ORIGIN      
        1 catttctcgt gccgatttca cctcgtatgc catgacttaa tttatgatcg gtatgtgaaa
       61 atgcattgta ggaggaatga catgatgcac atgatatcgt gctgtcggca gataatattg
      121 ggtcataaaa taatattctt cccaaagcta ccttcgcatc tgttaatgga ttctcatcaa
      181 agttgtacga agtaggcgga aaatacgagg cctgacgaaa tgtattcgtc tgcatggcca
      241 cggaggcact cagcaacaaa caacatccaa ttacaactaa acgcgatctc attggttgat
      301 tacaaatgaa gcactcatat aggcagcaag cgcatgtgcc ttcgcaccgg gaatttgcac
      361 cgtggcatcc atatcacagg tcactgtcga aaaaaatctt tccatgtcga gggtgacatt
      421 tattttttca ctcgttttgc agatcctgta tgtcactaag gctgtttgat caggtgcatg
      481 atatccgccg atatgatata ggaattcgcg tgctgtatca catcccgtcc tgatcccttc
      541 gatcttggca ttgatatacc cgctttgcca ggtccaatac atcccattta ccggatcaag
      601 cgctcctgct ttaggaccac tcatattcgt tgcactgtcg acaccaagtg tgaaacgaat
      661 gccattaaaa tgacctttgt atacaaaagg gatattcagc gatgatgaat cactcagatc
      721 tatcaatatg tatttcagtt caggctcacc aactactttc gatccatcga ggaggacaat
      781 atctacgata taaaatttgc aagtactcaa cgaaacagaa tcattcccat gaccggtggc
      841 atacatggta tgtaattgca taggctcacc ctgcatagtt gcattgaaag tgatcgtgcc
      901 gtacgacagt ttttccggct tggcaggaag cagcgatagt agggtaaaaa ggtagggtat
      961 catcggcctg tttcggtgta taaaggtagc taattaatta tctgaccatt gccaagcgcc
     1021 aaagtttaat ccccaaaaac ccccaaatca gacttcctaa aaatggcttg tttttcataa
     1081 aaacaggttt ttgcctgcta tatttttgta aggtgaaaaa aggttttcgc catattactt
     1141 cagttatagc ctttgtagtt attgcatttg caagcaccat tttgcaagcg cagacctata
     1201 atattgctac gtataatggc atgaccgtaa ccacctgttc agggaatttt tatgatagcg
     1261 gtggcagtgg cggaaactat ggcaataacg aaaattatgt agtcaccttt tgctctgcgt
     1321 ccggttcgcc attgtatttc gattttactt caagctcttc ggcattgaac ctggatgccg
     1381 gaacgggcga tacacttttc ttctacgatg gccttaccac ttccgatcct cttatcgcaa
     1441 ccctggtaaa ggctgatgac tatgctttca gttatcccgc tgtaggcact ttcagttcct
     1501 gtgttaccat taaatggaaa tccaatgctt ctgccgttga tggcggatgg caagcctcga
     1561 tatcatgcga agaaccgcca acctgtgccg ataatcctcc ggctgcagat gtattcggcc
     1621 aggctactgc ggtgtgtaat ctgaatgatt attgcggcac cacttccaga tattacggag
     1681 aagatgcacc atttaatttc ggcggaacag gaggttcttg tcccggtcct gatgatggtt
     1741 tatttgcagg taccctcgaa aataattcat ggctgaaatt tattgcagac gctacttcgg
     1801 catcattcga tttcaatgtt accggtggcg cttcctgcga tggcgtgcag gttggtattt
     1861 tttctttcga tgctgctaca aatactttcg caagaaaatc gccttgcaat tataccgatg
     1921 gcggatggtc cggtgcattc acgctgacag caacaggact caccgttgga caaacatatt
     1981 atatgatgat cgatggtaat gccgggtcga attgcgatta taccataggc gcaggaacag
     2041 gtgtagcaac cgtaagcgcc ggtgcggatc agatcgtgtg tgcgttcaat accacactcg
     2101 ctgcaacaac ggcaatgggc tccggaacct ggtcggttgt gagcggaaca ggggtgtttg
     2161 cagatgcaac aaatccaact acctatgttt ccggattgtc gtatggtatc aatgtattca
     2221 gatggacatc caccggcgga ttgtgtatgg acgttggcgc gtatgatgat gtggtggtgg
     2281 aaaatacgtg tttgctgcct atcacccttt cagattttac ggcaaccgtg gtggatgata
     2341 tggtgcggct caattggacc accgcatctg agatcgacaa tgatcatttt gtgcttgaac
     2401 gagccggtgc tgatcagcag ttcatggcca tcgcagagat catgggaagt ggcaccacta
     2461 ccattccggt gaactacact tatactgatg atatgccact gccggcggta aattattatc
     2521 gcttgaagca ggtggatgtc aatgccaata catcaacaag caacgttatt gcaattgaat
     2581 cacattcaga tgcacaaacc ttcactatcg gcgattgcta cttcgacaat gcatcagagg
     2641 tgctgaccat tccatatcag acctcggcat ctgttgcatt ggacattcaa ttgtgcgaca
     2701 tcgccggaag aattttattt tctgctacat gccataccac ggcagtaaat gataatgcgc
     2761 aagtagacct gcatcacctg acccaaacag gtttgatact cttgcaaatt accggtgata
     2821 atggcgtgcg ctccaatcat actttttgga tcagtgaatg atgtgggttg aattttataa
     2881 aaaattcata aagcaactct acattaattc aatgtttacc ataatggcgg taaaagtgca
     2941 cctcaccagg gcaaagtata atgaaaaatt atactaattt attcatattt ggatgaatta
     3001 tatcttttcg acagaaagca tacgaatcca tcctttgcat ataattttaa cccaatatat
     3061 tcctgcaggt tgatcggtca aatcgaagat attagtattt cctgagcatt caacttgatc
     3121 aaaaattaag gcaccattga tatcgctaat agaaattgtt acaaaagtcc cggaaggcaa
     3181 tccaatatta aaaatattgt ttgtgggatt aggaaatatt tcaatattcg gaaaagatcc
     3241 attctgcact actaatcctt cgtcagctaa aaaatcacaa tcgtcgtcta ttccattgag
     3301 caactcgatt gcgcctggaa atacagcagg attggtgtcg ttgcagtctg tagaatctag
     3361 aacatatccg gagatttcca tgcaggtcaa ggtgtctata ttcgcattac caaataaatc
     3421 accatctgca tcctgatata accatgtgaa agtcaaattg tcgtcaatta tgccgttgca
     3481 atcatcatca agcgcattgc atacctctgt ggtacccgga tatattgaag ccagtaaatc
     3541 gttgcaatcc aagtcatttg tcacataacc cggtacggcc tcaacacaag tatctataac
     3601 tattaacgga tttccaaagg tgtctccatc tgcatcaata tagaatgtat atacgatcaa
     3661 tccatcatca attaaagcat tgcaattatc atcaatacca ttgcatgact ccggggcatc
     3721 tgggttaatg tacaaatcgg catcattgca atctaagcta ttgttaacat aaccaggtgg
     3781 cgtaatacag gaggtgctgt ctatagaaac atccccataa ttatctccat cgaagtccaa
     3841 ataatataca ttaaatgtag catcctcatc aattaaacca ttgcagttgt cgtcaaaagc
     3901 attgcaaata tctattgctg aaggatattg tgaggcatct gtatcgttgc agtctgtgct
     3961 gtcggccaca tagccaacag gtgccgaaca agccaaggta tcagagagca aattgccata
     4021 accatcgccg tcggagtcag cgaaataatg attatcacag atagctaatt taacaatcca
     4081 aatatctgat aaaccataat ttgaatcaac atcgtaatcg tcagaattag aaactccccc
     4141 aacaagcaaa caattatcag gagtatttat tatagaatag gctttatcac tttctgaccc
     4201 gccaagcgat atctgtgatg taatctgaag cgatgaatca ataatcgcaa tccaaaaatc
     4261 tgcactacca aggttagccg ttaggtcgcc atcaaaggat tccgaatatc cacaagtagc
     4321 aattttatta atttggaatt ctgtgcatcc aaagaggcca tccccttcgt ggccgccaaa
     4381 tgattttgca tttatcaggc tcccaatata tgttttagta acccatgcat cccaatcgcc
     4441 aatatgatct gttacatcac catcatctga aagtataatt ccggttgtat atgcagtatc
     4501 tccaaaagcc gcacaggaga cgccttcatc gctatctgaa ccaccctgtg cggattgcca
     4561 aattaattca ccggtgtcat tgatgtaaat tgtccagaaa tcagaaaatc cgcccttaaa
     4621 atacgtaaca tcaccatccc cgccgccaca aacacccgtt acaatgaacc cggaatcagc
     4681 tttggaaatt ccatatgcat gatcatgttt tgtagaacca tacgaattgg accagataat
     4741 atcacccact gcatctattt taaacaccca aaaatctttg tagtcagtgg taccaacatg
     4801 accgatcaca tcgccatcgt tgctatatga ttgaccagca attacaaacg acccgtcatc
     4861 aagggaaata attccggcat ctgcagcatc aaatccggat ccgccataag ttttttgcca
     4921 aactatattc ccaatactat ctactcttac aacccaagcg cttacttcta aattatcagt
     4981 tacatcaaag tcgtttgaat acgtatatcc cgccataatg taaccgccat ctaatgtggt
     5041 atcaactcca tagcaccgat ctattgaact accaccatag gtttggatcc attcaatatc
     5101 acctgaacta ttcattttaa ttaaaagaaa atcagttgac cctttatttt ccggaatatc
     5161 aaaatcatct gaataggagt acccgcaaat aacgcaaccc ccatctcttg tttgtttgat
     5221 ctgttttaaa taatcgttat cggagccacc aatactcttt ccccagattt tttctaatcc
     5281 ttgtgaaaat gctcctacaa catacattga caagtaggta aataataaaa tatgttttag
     5341 gcttcgcatt tcttgtaggc ttgcaaatga attattgcaa tgaataatgt aaatttagct
     5401 attaatttca cttcaaactc aattgtcatg caaatgttaa atcccccccc cttaacattg
     5461 gaaaacagct acttgctgct gctttcattt taatttccat gcaactgtca ccggcatgga
     5521 gttcattgcc cttgaaaact aataatagca ccctaactga aaaatcaagc acatgcttgc
     5581 ctttggcttt cgatgaaagc aaattcccga ataactttag ttggctggtg tttcctgatt
     5641 caactttgtc agattccata gtttatgctg aaggagatac agttgaaatt tacacttcag
     5701 gtcctgattc cattgtagtc atatttgaat ctactgctcc ggatagttta ctggaggatg
     5761 cctggacaat ttctcccggt gacccgcgca tgttgatcca tgtcac
//
LOCUS       DAOQHY010000190         5803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_7628_length_5803_cov_6.698330, whole genome shotgun sequence.
ACCESSION   DAOQHY010000190 DAOQHY010000000
VERSION     DAOQHY010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5803
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7628_length_5803_cov_6.698330"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..680
                     /locus_tag="PLL28_12615"
     CDS             135..680
                     /locus_tag="PLL28_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNF70213.1"
                     /translation="MEATATKTKWVVDPTHSSIGFSVKHMMMTTVRGEFEKYAIEAET
                     EGDDFMTAAITFTAETASVNTRNEQRDGHLKSDDFFNAEKFPVISFVPTKFERVDNDD
                     MYTLYGDLTMRDVTKNIKLDVEFGGVMKNPWGSTVAGFIINGKINRKDWNLNWNAALE
                     AGGVLVSEDVRIHIELQLVAQ"
     gene            784..2337
                     /locus_tag="PLL28_12620"
     CDS             784..2337
                     /locus_tag="PLL28_12620"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70214.1"
                     /translation="MKSLTGILFLFCIHQRACAQLQLQWNTVNTEISESNPIVYAWQR
                     EDLSTHILCTDLGNNFLYNNVISISGDITIGQQLFYPDSLRMLSFKGLAYDNLNNFYV
                     AGMADLGLGGTHEVVAKYAPDLTLKWEYFSPDSVSLIKNAIHRSGDTTIVVSVSSVGM
                     LFTSLHNSGQQVETNFTYNNKYFEFPDLVAYPQNRIVMGFVSDSSILVYVYNGHGSAF
                     NDETIEIEGFTGLLPMSIAYDDSANYYFQLLGYDDEDSLSKIILQVNASASLNWMKST
                     AFHKNDLYATLDAKLYVDEDNTLIASTVTQTNDDSVYINMQRYDVAGTIINQRKLNAS
                     EWLDPDLSATSAYRASTDMLYVYFPRMQSPDYAGIISMSCNSDWMHVDTLDVAFSEAL
                     AQCYAFTDEEDILTLVNLKTDVGPLRNIEVSQFKDAYVGIHQIENPSPIEIFPNPCAD
                     YIICALKGQDLKVNSLVITDLAGRKLIELEDIYTDRVSINTSQLVPGVYQLTIIGSAF
                     RYSETLVKQ"
     gene            2465..3226
                     /locus_tag="PLL28_12625"
     CDS             2465..3226
                     /locus_tag="PLL28_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HNF70215.1"
                     /translation="MHRPILHQMTADMRSGLKKFAVLIDPDEVGPSELKRLVELGLEN
                     HVDYFFVGGSLVIGDAMDMTIRFLKEHCPIPVVIFPGSIMQVNPHADALLFLSLISGR
                     NPELLIGNQVLAAPYVKKSGMEAIPTGYILLDGGAPTTVSYISNTTPIPSDKPEIAAC
                     TAMAGELLGMQLMYMDAGSGAKKPIPADVIHAVRKSTDVPIIVGGGIKTPEKAYEDCN
                     AGADIIVVGNAIEKDTSLIHDIAFAIHSASVTSAG"
     gene            3255..4178
                     /gene="rsgA"
                     /locus_tag="PLL28_12630"
     CDS             3255..4178
                     /gene="rsgA"
                     /locus_tag="PLL28_12630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNF70216.1"
                     /translation="MQGLVIQSTGSFYDVQINGQQMVTCRLKGRLRLDDKKQTNPVAV
                     GDLVEVEQDEKLNYQIAEVHPRRNYIIRSDTHRKHIKQVIAANIDQTVVVASLRKPRT
                     AYGFIDRVLLTSEVYDIPAVVIFNKHDLYDDKDNALLEELMYGYKDAGYDCFSTSVTN
                     REHVEEIYAVFHNKTSLITGPSGAGKSSLVNMIEPDLNLRIGEISKYTEKGQHTTTFA
                     RMHHLPHGGAVIDTPGIKEFGLVDIDAEEVSHFFPEMRNRIGTCKFNNCLHINEDGCA
                     IKDMLENGELSVQRYTSYFNFYHEIKEAQKW"
     gene            4183..4635
                     /gene="dtd"
                     /locus_tag="PLL28_12635"
     CDS             4183..4635
                     /gene="dtd"
                     /locus_tag="PLL28_12635"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNF70217.1"
                     /translation="MRAVIQRVNSASVNIEGNCHAEIGKGFLVLLGVEEADGSEDVEW
                     LAQKIAGLRVFADEQGLMNINVQDAGGDILVVSQFTLFASTKKGNRPSFIRSAKPDIA
                     IPLYESFNKTLSTLIGKQIYTGIFGADMQVELVNDGPVTILMDTKNKE"
     gene            4640..5269
                     /locus_tag="PLL28_12640"
     CDS             4640..5269
                     /locus_tag="PLL28_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70218.1"
                     /translation="MKKIASKYFEVILIALTVLFLFLKPNLTNFGERISMLFFGTLSF
                     YYLASGILVFLDKNRVGRIMRLMYLFGLWSVSIMVLAIMVRIMLIQMDKALLITAISA
                     SLGLIAYITLYYRRLEGEDREALLYLIQPLFIRVVTSMLIGVAFLIASNYAVYSMFGT
                     HKRDPRYVEKIVSAYENPKDTTIVNDYKRYDEEINKVEEPSPSTNTEQP"
     gene            5280..5615
                     /locus_tag="PLL28_12645"
     CDS             5280..5615
                     /locus_tag="PLL28_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HNF70219.1"
                     /translation="MTIREAQETIDKWITTTGVRYFNELTNMAMLTEEVGEVARIIAR
                     KYGEQSFKESDKDKLLSDELADVLFVLICIANQTGVDLTEALEKNLDKKNVRDVERHK
                     NNDKLRGES"
BASE COUNT         1781 a         1124 c         1227 g         1671 t
ORIGIN      
        1 tgatacctga tacctgatac ttgatacctg atacctgata cttgatacct gatacttgat
       61 accaattaca gatactcatc atcgaagcac aaaaaaaatt gatattaaaa acaacaaaat
      121 aaaatcaaat taaaatggaa gcaacagcta caaaaacaaa atgggtggta gacccaacgc
      181 attcgtcgat cggattcagc gtaaaacaca tgatgatgac cactgtacgt ggcgaatttg
      241 aaaaatatgc tatcgaagct gaaactgaag gcgacgattt catgacagca gctattacat
      301 ttacagctga aactgcaagt gtgaatacac gcaatgaaca acgcgatggt cacttgaaga
      361 gcgatgattt cttcaatgca gaaaaatttc ctgtgatctc attcgttcca acaaaattcg
      421 aacgcgtaga caatgatgat atgtatacat tatatggcga tttaaccatg cgtgatgtaa
      481 caaaaaacat caaactggat gtagaatttg gcggtgtaat gaaaaatcct tggggcagca
      541 ctgttgccgg atttatcatc aacggtaaga tcaaccgtaa agattggaat ttgaactgga
      601 atgcagctct tgaagcaggt ggcgttttgg tgagcgaaga tgtgcgcatt cacattgaac
      661 ttcaattagt tgcgcaataa ataattcgga cttataaaaa agcctcttcg aaagaagggg
      721 ctttttttat gtatttatcg agcgtttggt tatattgcaa agcaaaccac aaccgcagaa
      781 accatgaaat cattaacagg catcttattt ttgttttgca ttcatcaacg tgcatgcgct
      841 caattacaat tacaatggaa cactgtaaac actgaaatat ccgaatctaa tccgatagta
      901 tatgcctggc aacgcgaaga tttatctaca catatccttt gcaccgatct gggaaataat
      961 ttcttgtata acaatgttat ctccatttcc ggggatataa caattggaca acaattattt
     1021 tatcctgact cactgcgtat gctatccttt aaagggttgg cttatgataa cttaaataat
     1081 ttttatgttg ccggcatggc cgatttaggg cttggtggga ctcatgaagt tgtagctaaa
     1141 tatgcacccg acctcactct aaaatgggaa tatttctcgc ccgattcagt ctctttaata
     1201 aaaaacgcca tccatcgctc cggcgatacc acaattgttg tatcggtaag tagtgtcggt
     1261 atgctattta cttcattaca taattcaggg cagcaggtag aaaccaattt tacttataac
     1321 aataaatatt ttgaattccc cgatcttgtc gcgtatccgc aaaatcgaat tgttatggga
     1381 tttgtttccg acagttcaat attggtatat gtttacaatg gacatgggag tgcttttaat
     1441 gatgaaacaa ttgaaattga aggctttacg ggtttgttac ctatgtccat cgcttatgat
     1501 gacagtgcaa attactattt tcaattatta ggatatgatg atgaagattc cctttcaaaa
     1561 ataatcctgc aggtgaatgc atccgcttct ttgaattgga tgaagtcaac tgcatttcat
     1621 aaaaatgatt tgtatgcaac gctcgatgca aaattgtatg ttgatgaaga caacacgctg
     1681 attgcaagta cggtaactca aacaaatgat gacagtgttt atatcaatat gcagcgatat
     1741 gatgttgcag gtacaataat taatcagcgc aaattaaatg catctgaatg gttagatccg
     1801 gatctttcag ccacatccgc ataccgcgca tccacagata tgttgtatgt atattttcct
     1861 cgcatgcaat ctcctgatta cgcaggcatc attagcatgt cttgcaatag cgattggatg
     1921 cacgtagata ctttagatgt tgcattttcc gaagcgcttg cgcaatgcta tgcttttact
     1981 gatgaggaag atatacttac attagtcaac ctgaaaaccg acgtaggtcc actgcgaaac
     2041 atcgaagtgt cgcaatttaa agatgcgtat gtaggcatcc atcagattga aaatccatca
     2101 ccaatcgaaa tttttccaaa tccttgtgct gattatatta tttgtgcgtt gaaagggcag
     2161 gatcttaaag ttaattcgtt ggtcatcaca gatttggcag gaaggaagct tattgaattg
     2221 gaagatattt atacagatcg tgtttccatc aacacttcac aactcgttcc gggggtatat
     2281 cagttaacca tcattggctc tgcgttcaga tacagtgaga cgctggtcaa acagtgattc
     2341 cccttcaccg gttaggctga ccatatgcgg gaggttcctt ttcaagcctt tccactgccg
     2401 attgagcggg ttttaaagtc gcttattcca cgtctttttc actatttttg ctccgatcac
     2461 catcatgcat cgccctattc tacatcaaat gaccgccgat atgcggagcg gactgaagaa
     2521 gttcgctgtg ctcatcgacc cggacgaggt tggcccttcg gagctgaaac gcttggtgga
     2581 gttgggtttg gagaatcatg ttgactattt ctttgtaggc ggaagcttgg tgattggcga
     2641 tgcgatggat atgaccattc gctttttgaa ggagcattgc cccattccgg tagtgatatt
     2701 ccccggaagc attatgcaag ttaatcctca tgccgacgca ttgctttttt tgagtctgat
     2761 aagcggcaga aaccctgaat tgctcattgg caaccaggtt ttggcggcac catatgttaa
     2821 aaaatcaggg atggaagcca ttcctaccgg atatattctc ttagatggcg gagcacctac
     2881 tacggtaagt tatatctcca acaccacacc tataccttca gacaagccgg aaattgcagc
     2941 ttgcacagca atggcaggtg aactccttgg tatgcaattg atgtatatgg acgcgggcag
     3001 cggggcgaaa aaaccaattc ctgccgatgt aattcacgct gttagaaaaa gcactgatgt
     3061 acctatcatt gttggcggtg gtattaaaac accggaaaaa gcttatgaag attgcaatgc
     3121 cggtgccgat atcattgttg taggcaatgc cattgaaaaa gacacttcgc tcattcacga
     3181 catcgctttt gccattcact cagcttcggt gacatctgcc ggatgaagcg gcgagtaaac
     3241 acccacatat actgatgcag ggtctcgtta ttcaaagtac aggaagtttt tacgacgttc
     3301 agatcaatgg acaacaaatg gtgacctgtc gccttaaagg aaggttgcga cttgatgata
     3361 agaagcaaac caatcccgtt gcagttggcg atttggtgga agtggagcag gatgaaaaat
     3421 taaattatca gatagctgaa gtacatccgc gaagaaatta tattatccgc agtgataccc
     3481 atcgcaaaca tatcaaacaa gtaattgcgg cgaatatcga tcaaactgtt gttgttgcca
     3541 gtttaagaaa accacgaact gcttacggat ttattgaccg ggtattgctt acaagtgaag
     3601 tgtacgatat tcctgctgtt gttattttta ataaacacga cctctatgat gataaagaca
     3661 atgcattact tgaagaattg atgtatggat ataaagatgc gggatatgat tgcttcagca
     3721 cttcagtaac aaacagagag catgttgaag aaatctatgc tgtatttcat aataaaacat
     3781 ctctcattac agggccttcc ggtgccggaa aatcatcatt ggtaaatatg atagagcctg
     3841 atttgaattt acgcatcggg gaaatttcaa aatacacaga aaaaggtcag catactacca
     3901 catttgcgcg tatgcaccat ttgccacatg gaggtgctgt gatagataca ccgggcataa
     3961 aagaatttgg acttgttgat atcgatgcag aagaagtaag tcactttttt cccgaaatgc
     4021 ggaatcgcat aggcacatgt aaattcaata attgtcttca cattaatgaa gatggttgtg
     4081 caattaaaga tatgttagaa aacggcgaat tatctgttca gcgttatacc agttatttta
     4141 atttttatca cgagatcaag gaagctcaaa aatggtgaag ttatgagagc agtgatacaa
     4201 agagttaaca gtgcatctgt caatatagaa ggcaattgtc atgcagaaat cggaaaaggt
     4261 tttctagtat tgcttggcgt tgaagaagca gacggttcag aagacgttga gtggcttgca
     4321 cagaagattg cggggctgcg ggttttcgca gatgagcagg gtttgatgaa tatcaatgta
     4381 caggatgccg gcggtgatat actggttgta agccaattca cacttttcgc atcgacaaaa
     4441 aaaggcaatc gcccatcctt tatacgaagc gccaagcccg atattgcgat tcccttatat
     4501 gagtcattca ataaaacatt gagtacactc atcggaaaac agatatacac cggtatcttt
     4561 ggtgccgata tgcaggtgga gttggtgaat gacggaccgg taaccattct gatggataca
     4621 aaaaataaag agtaaaaaag tgaaaaaaat cgcgtcgaaa tattttgaag taatactgat
     4681 agcgcttacg gtattatttc tttttctaaa acctaatttg acaaatttcg gtgagcgcat
     4741 cagcatgctg ttcttcggta cattatcatt ttattatctc gcttcaggca tattagtttt
     4801 tctggataaa aatcgcgtgg gaagaatcat gcgattgatg tatctttttg ggctttggtc
     4861 tgtttccata atggtattgg caattatggt gcgcatcatg ctgatacaaa tggataaggc
     4921 attgctgatc accgctattt cagcttcact gggactaata gcttatatca ctttgtatta
     4981 ccgccgactg gaaggtgaag accgcgaagc attgttatat ttaatacaac cattatttat
     5041 tcgtgtagta acttccatgt taattggtgt tgcattttta attgcaagta attatgctgt
     5101 gtatagcatg tttggaacgc acaaacgcga tccgcgttat gtcgaaaaaa ttgtaagcgc
     5161 atacgaaaac ccaaaggata ccaccatagt aaacgactat aaacgttacg atgaggaaat
     5221 aaataaggtt gaagagcctt ctccatccac aaataccgaa caaccataaa tataaagcca
     5281 tgaccattcg tgaagctcag gaaactatcg acaagtggat taccactacc ggcgtccgct
     5341 attttaatga gcttaccaat atggccatgc tgactgaaga agtgggtgag gtggcccgta
     5401 ttattgccag aaaatatggc gagcaatcgt ttaaggaaag cgataaagac aagctattat
     5461 ctgatgaatt agccgatgtt ctattcgtgc ttatttgcat agccaatcaa accggtgtag
     5521 acctcaccga agccctcgaa aaaaatcttg ataagaaaaa tgtcagggac gttgagcggc
     5581 ataaaaataa cgataagctt cgcggtgaat cgtgaggcgt gagacgtgaa tcgtgactcg
     5641 tgattcgtga ctcgtgaata gtgaaataat ccttcgaatc ttctattctt ccacccttcg
     5701 aatcttcgac tcttcaaacc ttcgaatctt taccctctct gcgcctccgc gtattctaag
     5761 gtacccattt caccaaattt atttcaggct gccactaaat aat
//
LOCUS       DAOQHY010000191         5737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_7752_length_5737_cov_5.493312, whole genome shotgun sequence.
ACCESSION   DAOQHY010000191 DAOQHY010000000
VERSION     DAOQHY010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7752_length_5737_cov_5.493312"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="PLL28_12650"
     CDS             complement(<1..801)
                     /locus_tag="PLL28_12650"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNF70220.1"
                     /translation="MKKNVAVVCGGYTAEREISLGSGDVVIRNLDESPFNVFKVVIDP
                     DGWFVEPEHIPVDMADFSFLWTGNVVKFDLVFNAIHGSPGEDGKLQGYLDMMGVPYTN
                     CGVATSALTFNKFWTKQVLDNHQIPMAKGRLVNKQNAEAMEQEISSQFQLPLFVKPNN
                     NGSSFGVSKVKDMRELSKAISEALKYDREALVEEGISGREVTCGVYRHEGNIKVLPIC
                     EVVTGKHDFFDYTAKYTAGESDEIIPAPIPETEAIRVRNYSTAIYEALN"
     gene            complement(919..1266)
                     /locus_tag="PLL28_12655"
     CDS             complement(919..1266)
                     /locus_tag="PLL28_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNF70221.1"
                     /translation="MAIHITSDCINCGACEPECPNNAIYEGGVEWAFADGTTVKGSVT
                     MMDGSSMDADQKNAPIADDIYYIVPDKCTECQGFHEEPQCAAVCPVDCCLPDPDHSET
                     PDQLLAKKAKMHI"
     gene            1409..2407
                     /locus_tag="PLL28_12660"
     CDS             1409..2407
                     /locus_tag="PLL28_12660"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HNF70222.1"
                     /translation="MDTENRIAALVSLGECIQTYPDDLSEVVTRMGHVNGWFTETECK
                     RMLSSICGEFLQRDLLHTWMSNYQATETQKTIGIVMAGNVPFVGMHDLLCIIMSGHKA
                     QVKMSSKDNLFFPWMKEALSKISPPVSEQITFTEKLENFDAVIATGSNNSARYFEYYF
                     GKYPHIIRKNRTSIAILTGNESDDALYNLGKDVFWYYGLGCRNVGKVFIPEGYDKQHF
                     FRLWEDYRYVVDNTKYKHNYDYNRALLLLNKTNYLTNDFYMLVDSDQLYSPLSVLYAE
                     TYLHDADLQLKLESIGENLQCVVSSAPGNTPFGKTQEPRLSDYADHVDTMQFLMQV"
     gene            complement(2449..3729)
                     /locus_tag="PLL28_12665"
     CDS             complement(2449..3729)
                     /locus_tag="PLL28_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNF70223.1"
                     /translation="MDTRRSFLRKSAALASLTAFTDLVLDDTLKAEVARVQHLDPLTV
                     ANDEEFWMNIRQAYTVSSTIINLNNGGVSPAPKVVQDAVDRYNKLTAEGPSYYMWQIL
                     DKGRESLRRKIAELSGADPEEIAINRNTTEALDNVIFGLDFEKGDEILLTKQDYPNMI
                     NAWKQREKRDGIVLKWIELDLPTDDVLYLVKKYTEQITSKTKLVQVNHIINWTGHINP
                     VKEIADAVHAKGPEVMIDGAHTMGHFMFNVPELGGDYYGSSLHKWTSASVGSGMLWIK
                     KEKIHKVWPLMGAPDTQDVNNIRKFENLGTRSFPIEYAIANAIDFHNMIGAARKEARL
                     RYLKEHWVNQCLDLPKFHLNTSMDPRFSCAIANFRIDGMEPGDIASKLITDYNIHCVA
                     INWENIHGVRIAPHVYTMPSELDLLAKAIKKLAS"
     gene            complement(3775..4914)
                     /locus_tag="PLL28_12670"
     CDS             complement(3775..4914)
                     /locus_tag="PLL28_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNF70224.1"
                     /translation="MTMRVYLDNAATTALDPQVLETMLPFLQQHHGNPSSIHSFGRET
                     RAAIEKARKQIAKILNVSPGEIFFTSGGTEANNMALQCSVRDLDVKHIITSPIEHHAV
                     THTVEALQSEGKIILSYVNILPDGSIDYAHLEQLLQLPNGKTLVSLMHANNEIGVLLD
                     MEKVSAMCIQYHALFHCDTVQTLAHYPLDLQATKVTFISCAAHKFHGPKGVGFIYING
                     DAAIKPLIHGGAQERNMRAGTENLYGIIGLAKALEIAYQELDTTRAHITALRTHMAKR
                     LRDEIPGIIFNCPDDGLYTVLNVNFPPSESNELLLFNLDIAGIAVSGGSACSSGSNVG
                     SHVIARLNREQNSAAVRFSFSKFNTMEEIDYTVEKVKALFPAHVA"
     gene            complement(4955..>5737)
                     /locus_tag="PLL28_12675"
     CDS             complement(4955..>5737)
                     /locus_tag="PLL28_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNF70225.1"
                     /translation="VALGVTDVVELFTNPNGRFPHNPEPLPEHLSTIAHEVKHQKADV
                     GLVVDPDVDRLAIICENGDMFGEEYSLVAIADYVLQKRKGDVVSNLSSTQALRDVANK
                     YKVAHHQSAVGEVHVVQKMKEVNAVIGGEGNGGIILPELHYGRDALVGIALFLSHLAT
                     SGKSCTRLRAEYPNYFMAKNKIELPEGTNVQDILVQMEEKYKKEHLNKTDGLKIEMGS
                     DWVHLRASNTEPIIRIYSESTTKTTAENINRKIMDDIKMLMK"
BASE COUNT         1551 a         1257 c         1200 g         1729 t
ORIGIN      
        1 gttcaatgcc tcataaatag cggttgagta gttgcgtacc ctaatcgcct ctgtttcagg
       61 aattggggcc ggaatgatct catcactctc tcctgccgtg tattttgccg tgtagtcaaa
      121 gaaatcatgc ttgccggtta ctacctcaca aataggcaac actttaatat ttccttcatg
      181 tctatatacc ccacaggtca cttctctacc gcttataccc tcttcaacca aggcttcgcg
      241 gtcgtatttc agtgcttctg agattgcctt cgacagttcg cgcatatctt tcaccttcga
      301 aacgccgaag ctggagccgt tgttattagg ttttacgaat agcggcaatt gaaactgact
      361 actgatttcc tgctccatgg cctcggcatt ttgcttgttt accaacctgc ctttagccat
      421 gggaatctgg tgattatcga gcacttgttt ggtccagaat ttattgaatg taagtgctga
      481 tgtagctaca ccgcaattgg tatatggcac acccatcata tccagatatc cttgcaattt
      541 cccgtcctca ccgggagagc cgtgaatagc attaaaaacc agatcaaatt tcaccacatt
      601 accggtccac aaaaagctga aatccgccat atcaaccggt atatgctccg gttctacaaa
      661 ccagccatcc ggatcgataa caactttaaa tacattgaat ggcgattcat cgaggttacg
      721 tatcactaca tcgccactcc ctaatgaaat ttcccgttca gcggtatatc cgccgcagac
      781 cacggcaaca ttctttttca tggtggcaaa ttaacgcaaa aggtgatgcc tgttgtatct
      841 gacttgtaaa caattgatag tggtaaaata aaaaaaggcg ccacccggag gaagcgcctt
      901 tcattaatat gaggtttttc aaatgtgcat ttttgctttc ttcgcaagaa gctgatcagg
      961 tgtttcagaa tgatcaggat caggaagaca acagtcaacc ggacaaactg ctgcacattg
     1021 tggttcttcg tggaaccctt gacattcggt gcatttgtcg ggaacgatgt aataaatatc
     1081 atctgcaata ggtgcgttct tttggtcggc atccatggaa gagccatcca tcatggttac
     1141 tgatccttta acggtggtgc catcggcaaa agcccactcc acgccacctt catagatcgc
     1201 attatttgga cactcgggtt cacaagcccc gcagttgata cagtctgatg ttatatgaat
     1261 tgccataata agtctttcgc tatttttgag cgaacaaata taaatgcagg ctcgtcacat
     1321 tctcaaaaaa acagcctttt taggactgaa tttatactca ttctaaataa cgttgttgtt
     1381 gtgacgggtg tttaatactg aaatgactat ggatactgaa aatcgcattg ctgcgctggt
     1441 gagccttggt gaatgtatac agacctatcc ggacgatctg tctgaggtag ttacgcgcat
     1501 ggggcacgtc aacggatggt tcacggaaac ggaatgcaaa cgtatgttat cgagcatttg
     1561 cggtgaattt ttgcagcgag atttacttca cacctggatg tcgaattatc aggctactga
     1621 aacccaaaaa acaataggca ttgtaatggc cggaaatgtg ccttttgtag gtatgcatga
     1681 tttgttgtgc attattatga gtggacataa ggcgcaagtg aaaatgagct caaaagacaa
     1741 tctttttttt ccgtggatga aggaggcact atcaaagatt tcacctcctg tttccgaaca
     1801 gatcactttt accgaaaagc tcgaaaattt tgatgcggtg attgcaaccg gcagcaataa
     1861 ttcggcgcgt tattttgaat attatttcgg caagtatccg catatcatcc gcaaaaacag
     1921 aacttctatt gcaattttaa ccggaaatga gtctgatgat gcattatata atttaggtaa
     1981 agatgttttt tggtattatg gacttggatg tagaaacgta ggaaaagttt ttattcccga
     2041 agggtatgat aagcaacact tcttcaggtt atgggaagat tatcgatatg tagttgacaa
     2101 taccaaatac aaacataatt acgattataa tcgcgcatta ctcttattga ataaaaccaa
     2161 ttacctcacc aacgattttt atatgttggt agacagtgac caactatatt caccattatc
     2221 ggtgttgtat gcagaaacat atttacatga tgctgatcta cagctaaaat tggaatccat
     2281 tggagaaaac cttcagtgtg ttgtttcctc tgctccggga aatacccctt tcggaaaaac
     2341 tcaagagccg cgtttatccg attatgccga tcatgtggat accatgcaat ttttaatgca
     2401 ggtttgaagt tgatagatac attgctatct aagaaaaaac tgtattgcct aactcgccag
     2461 ttttttaata gctttcgcta acagatctag ttctgaaggc atggtgtaca catgaggtgc
     2521 aatgcgcaca ccgtgaatat tttcccagtt aatggcaacg caatgtatat tgtaatcggt
     2581 aattaatttc gaggcaatat caccgggttc catgccatca attctgaaat ttgcgatagc
     2641 acaggaaaac cttgggtcca tgcttgtatt gagatgaaat ttaggcaagt cgaggcattg
     2701 attcacccaa tgttctttta agtaacgcaa tcttgcttcc tttctcgcag cgccaatcat
     2761 attatgaaaa tcaatggcat tggcaatggc atattctatc ggaaacgaac gtgtaccaag
     2821 attttcaaat ttgcgaatat tgttgacatc ttgcgtatcc ggtgcaccca tgagtggcca
     2881 tactttgtga atcttttctt ttttaatcca taacatgcca ctacctaccg aagcactggt
     2941 ccatttgtgt aaacttgaac cataataatc accaccgagc tccggaacat taaacataaa
     3001 atgccccatg gtatgtgccc catcaatcat tacctcaggt cctttcgcat gaacggcatc
     3061 tgcaatttct ttaaccgggt tgatatggcc tgtccaattg atgatatgat ttacctgaac
     3121 caattttgtt tttgaagtaa tttgctccgt gtattttttt acgaggtaca acacatcatc
     3181 tgttggcaaa tcaagttcta tccatttaag tacaatgcca tctcgtttct cacgctgctt
     3241 ccaggcattg atcatattcg gataatcttg tttagtaagc aaaatctcat cacccttttc
     3301 aaaatctagt ccgaaaatca cgttatcaag cgcttcagtg gtatttctgt tgatggcaat
     3361 ttcttcgggg tcggcgccgg ataattctgc aatttttcta cggaggcttt cacgtccttt
     3421 atccagtatt tgccacatat aataactcgg accttcggca gtgagtttat tatatcgatc
     3481 aacagcatct tgcacaactt ttggtgccgg acttactccg ccgttattca gattaataat
     3541 agtagatgaa acggtatatg cctgacgaat attcatccag aactcctcgt cgttggcaac
     3601 agttagcgga tcgaggtgtt gtacgcgtgc gacttctgct ttgagtgtat catccaaaac
     3661 aaggtcggta aaagctgtca gcgatgcgag cgctgctgat ttacggagaa atgatctgcg
     3721 ggtatccata ggagggtatt ctcctgcaag ataacacatt tcaaattgtg ggaatcaggc
     3781 tacgtgggca ggaaatagtg ctttcacttt ttcaacggtg tagtcaattt cttccatggt
     3841 attgaatttg ctgaaagaaa agcgcactgc cgcactattt tgctctctat taagtcttgc
     3901 aatcacatgc gagccgacat tacttccgct gctgcatgcg cttccgccac tcactgctat
     3961 tccggcaata tcaagattaa ataaaagaag ctcgttgctt tcagaaggag gaaaattcac
     4021 gttaagaacg gtatacaaac catcatccgg acaattaaaa atgataccgg gaatttcatc
     4081 gcgcagccgc ttcgccatat gtgtgcgcag agctgtgata tgtgcacgcg ttgtatcgag
     4141 ctcctgatac gctatttcca aagcttttgc caatccgatg ataccgtata aattttcggt
     4201 accggcgcgc atattgcgtt cctgtgcacc gccgtgaatt aatggtttta tagctgcatc
     4261 gccattgata taaataaaac ctacgccttt tgggccgtga aatttatgcg cagcgcagga
     4321 aatgaaagta acctttgttg cctgcagatc gagtggatag tgagcaagcg tttgcactgt
     4381 atcgcagtga aataatgcat gatattgtat gcacatggca ctcacctttt ccatatcgag
     4441 cagcacgcct atttcattat tggcatgcat caggcttacc agagttttac cattcggcaa
     4501 ttgcaataat tgttcgagat gcgcataatc tattgaaccg tcaggaagaa tatttacata
     4561 actaagaatg attttccctt ccgattgcag cgcttcaacg gtatgtgtta cggcgtgatg
     4621 ttctattggc gaggtgatga tatgtttcac atccagatcg cgcacactgc actgtaacgc
     4681 catattattg gcttcggtgc cgccgctggt aaagaaaatc tctcctggcg acacattcaa
     4741 tattttggct atttgttttc tcgccttttc aatggcagcg cgggtttcac gcccgaaaga
     4801 atggatagac gaggggttgc catgatgttg ctgcaagaat ggcaacatgg tctccagcac
     4861 ctgagggtca agggctgtgg tggctgcatt atcgaggtaa acgcgcatgg tcataggtag
     4921 tcaattacaa aactagcaga atttcagttc ccgcctattt cattaacatt ttgatatcat
     4981 ccatgatctt gcggttgata ttctccgctg tggtcttggt ggtgctctct gaataaatcc
     5041 taatgatggg ctctgtattg ctggcgcgaa ggtgcaccca atcgctaccc atctctattt
     5101 tgaggccatc ggttttattc aaatgctctt ttttatactt ttcttccatt tgcacaagaa
     5161 tatcctgcac gttggtgcct tcgggaagct caattttatt tttggccatg aagtaattcg
     5221 ggtattcggc acgcaagcgg gtgcaagatt taccggatgt tgccaggtgc gacaagaaaa
     5281 gtgcaattcc taccaatgca tcccgaccat agtgtaattc aggtaatatg ataccgccat
     5341 tgccttcacc accgataaca gcatttacct ctttcatttt ttgcactacg tgtacttccc
     5401 cgacagcact ttgatgatgt gctactttat atttgttggc aacgtcgcgc aaagcctgag
     5461 tactggataa attggatact acatcgcctt tgcgtttttg taatacataa tcggcaatgg
     5521 ctaccagtga atattcctca ccaaacatgt cgccattttc gcagatgata gcaagacggt
     5581 caacatctgg atctacaaca aggcctacat ctgctttttg gtgtttcact tcgtgcgcaa
     5641 tggtactcag gtgttcgggc agtggttcgg gattatgcgg gaaacggccg tttggattgg
     5701 taaacaattc taccacatca gtaacaccta gagcaac
//
LOCUS       DAOQHY010000192         5702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_7825_length_5702_cov_5.112803, whole genome shotgun sequence.
ACCESSION   DAOQHY010000192 DAOQHY010000000
VERSION     DAOQHY010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7825_length_5702_cov_5.112803"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..1347)
                     /locus_tag="PLL28_12680"
     CDS             complement(112..1347)
                     /locus_tag="PLL28_12680"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNF70226.1"
                     /translation="MWFEYFVSNRIMHNKERSFSRLIMGIARVAIALSLSVMLISTSL
                     VNGFRATISEKVYGFWGHINIAKQSLRNSFDDIPIYRQQEFINEVDSIPGVTGIQIYA
                     RKAAIIKTKTAMEGVILKGIGSDFNWDGFNKYIIDGHGIDLTKDEASRGTLLSKTTAE
                     RLDYHVGDSVLVHIIDQKENGDYQQMFRKLRIDGIYNTGLSEFDQLFMLVDIRHIQRL
                     NNWNADQIGGYEVFIDDPDRLDELEHEVHKAADPFWDVQTIKEIIPSVFDWLNLQKVN
                     EWIILVLMFMVALINMVTSLLILILERTNMIGILKAMGANNTAIRRIFLLNASNIIVR
                     GMLWGNIIGIGLCFLQQQFGLIKLPEESYYVSVAPVQLDWVWILMLNLGTFLVSIIFL
                     IIPSLFVSSIRPIKAIRFN"
     gene            1393..2619
                     /locus_tag="PLL28_12685"
     CDS             1393..2619
                     /locus_tag="PLL28_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNF70227.1"
                     /translation="MLLFNLMLAGLLNTAACKNSDSVTVSAIDTVHTDTHNDDAILTG
                     AEQTELWMPMLKGKEVALVVNQTSVIGKKHLVDTLIAAGVHISTIFAPEHGFRGTEDA
                     GATILNAKDTATGIPVLSLYGNKKKPLPQDLADADIIIFDIQDIGARFYTYISTLHYV
                     MESCAENKKKLLILDRPNPNGYYVDGPVLEKAYTSFVGMHQIPIVHGMTIGEYARMVN
                     GEHWLTNGVQCELQVIPCANYDHTDIYNLDIPTSPNMRTMQAIYLYPTLCLFEGTNVS
                     VGRGTDKPFEYIGSPYLPPTGFSFVPKSGSGAKTPPFMGQTCNGYDLSKWPVDSVRSG
                     KINLAFFLEVYHNYASSTPFFLENNFFNKLAGNAELMQQIKAGLSEEEIRLSWQDDIK
                     AFKQVRKKYLLYPDFE"
     gene            2631..3536
                     /gene="fmt"
                     /locus_tag="PLL28_12690"
     CDS             2631..3536
                     /gene="fmt"
                     /locus_tag="PLL28_12690"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNF70228.1"
                     /translation="MRIIYMGTPDFAVPGLQKLVEAGFNVVAVVTAPDKPSGRGLQVK
                     PTPVKVYADSQGIPVLQPVKLKDPEFISTLRTYNADLQVVVAFRMLPEVVWNMPPKGT
                     INLHASLLPDYRGAAPINHAIINGEKETGATTFFLQHAIDTGDLIDQIRIPIHDDDNA
                     GSIHDTLMMLGADLIVKTVIAIEENNYSTKPQSGESNKIAPKIFRENCRIDWNQPAAQ
                     IHNVIRGLSPYPGAFTDIKGPQWKILKSKFEIEPHQQSPGTIATDAKTYLKIASADGW
                     VYVLAIQPEGKRNMDIATFLHGYRS"
     gene            complement(3563..4519)
                     /locus_tag="PLL28_12695"
     CDS             complement(3563..4519)
                     /locus_tag="PLL28_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNF70229.1"
                     /translation="MIKYYKKIDNQIVEIDRPEKDCWINVYPPFDHKRLATLSDAIDI
                     PIDYLLDSIDINERSRFEQDDNVKLIVINTPIENDMENSIDNDAFYITIPIGIILTQD
                     YNIIISSAQNKVIDWFFSIAIKHLSPHDKDLVVLKIFEKNVFYFIQYLNEMNKRRYLI
                     EKELMHSSRNTELAKLLNIQKSLVYFVTDLRANELLMMKIQRTNFLGIKNDEEKNDYL
                     TDIVIDSSQALEMATVYTNILNGTMDAFGSIISNNLNSVMKRLTSITIILMVPTLIAS
                     FYGMNFANLPLENSNSGFYVIMLFSILISFALYWVFRRIRWF"
     gene            complement(4716..>5702)
                     /locus_tag="PLL28_12700"
     CDS             complement(4716..>5702)
                     /locus_tag="PLL28_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70230.1"
                     /translation="FPCLAPYWTIRGGFTETVMLIKDAGLYEESSWTIGTIDPVVHGR
                     ALTWSGYCLLFVFDLERGFAMLQAAQNILTATNDIYNLTYAYCFDGCYGAYMQKEDAE
                     EKILKGFELLERQNNPLINCMFYCWSDEYFRQKGQPEIRDKNLNLAASIAKEHGITYI
                     QGAVMLIRATIATSEPEMDSDKLIQIGQEMFMIFPEKGYQGFKAASKQFQAIGYLNKG
                     DIIAARNMIKESAEFVKLSGELESEFYLCMLLVHILALGGDRKHAFSIFGALHTFIEQ
                     SQYPVIGGAAVQYQTISQLLQPYEAQPECQSWFESGKSMPLTSAVNMGLAQI"
BASE COUNT         1721 a         1265 c         1123 g         1593 t
ORIGIN      
        1 gtatctagaa ccaagtatct agtatctaga ctaggtatca ggtaaaaatt cacgagtcac
       61 gaatcacgaa tcacgactca cgattcacaa ctcaccactc accactcacc atcaattaaa
      121 tcttatcgct ttaatcggcc tgatgctcga tacaaacaag gaaggaataa tgagaaatat
      181 gatagatacc agaaatgtgc cgagattcaa catcaatatc caaacccaat ccagctgcac
      241 cggtgcaacc gatacgtagt aagactcttc aggtaatttg ataagtccga attgttgctg
      301 caaaaaacaa agcccgatac cgataatatt accccacaac atcccgcgca caataatatt
      361 cgaagcattc aataaaaata ttcttctgat agcagtgttg ttggcgccca ttgccttaag
      421 tatgcctatc atgtttgtac gctcaaggat taaaatcaat aatgaggtga ccatattgat
      481 gagcgctacc ataaacatga gcaccaaaat aatccactca tttacttttt gcagattcag
      541 ccaatcaaaa acactgggaa taatttcttt aatggtttgc acatcccaaa aagggtctgc
      601 cgctttgtgc acttcgtgtt caagttcatc aagtctatcc ggatcatcaa taaatacttc
      661 gtatccaccg atctgatcgg cattccaatt atttaaccgt tggatatgac gaatatctac
      721 caacatgaat aattgatcga attccgataa tcccgtatta taaataccat caatacgcaa
      781 cttgcgaaac atctgctgat aatcgccatt ttctttttga tcgatgatat gtactagtac
      841 actatcacca acatggtagt ccaaacgctc ggcagttgtc ttcgagagca gtgtgccgcg
      901 tgaagcttca tcttttgtga ggtctatgcc atgaccgtcg atgatatatt tattaaaacc
      961 atcccaatta aaatcgctgc ctatgccttt taatatcact ccttccatgg cagttttagt
     1021 tttgatgatg gccgctttcc gcgcataaat ctgaatgccc gtaacacccg gaatagaatc
     1081 tacctcatta ataaattcct gctgacgata aatgggaata tcgtcaaatg aattgcgcaa
     1141 actctgcttc gcgatattga tatgtcccca gaagccataa actttttcgc tgatggttgc
     1201 acgaaatcca tttaccagcg atgtagaaat cagcataacc gataaactca atgcaatggc
     1261 cacgcgcgcg atacccatga tgagtcgcga aaagctacgc tccttattat gcatgatgcg
     1321 attgctgaca aaatattcaa accacatccc gattatcgtt tacctttggc ctttgatttg
     1381 ctatacatgc gtatgctttt gttcaatttg atgcttgcag ggctgctaaa caccgctgcc
     1441 tgtaaaaata gtgattccgt gacggtttcc gccatcgaca ctgtgcatac cgatacgcac
     1501 aatgatgatg ccatactcac cggtgcagaa caaactgaac tgtggatgcc aatgctcaaa
     1561 ggaaaagagg ttgcactggt ggtaaatcaa acttctgtaa tcggtaaaaa acatcttgta
     1621 gatacactca ttgccgccgg cgtgcatatc agtaccattt ttgctccgga acatggcttt
     1681 cgcggcacgg aggatgcagg tgctaccatt ctgaatgcca aggataccgc aacaggcatt
     1741 ccggtattgt cgctctacgg caataaaaag aaacctctgc cgcaagacct tgccgacgct
     1801 gatatcatca tatttgatat tcaggatatc ggcgctagat tctataccta catttcaacg
     1861 ctgcattatg taatggaatc atgtgcagaa aataaaaaga aactcctcat cctggatcgt
     1921 ccaaatccga atggttatta tgttgacggt ccggtattgg aaaaagcgta tacttcgttt
     1981 gtcggcatgc atcaaattcc catcgtgcac ggcatgacaa taggtgaata tgcacgcatg
     2041 gtgaatggtg aacactggct tacaaacggc gtacaatgcg aattacaagt tattccgtgt
     2101 gctaattacg atcacaccga tatttacaac ttagatattc ctaccagtcc gaacatgcgc
     2161 accatgcagg cgatatattt atatccaacc ttatgcttgt ttgaaggaac gaacgtgagt
     2221 gttggccgcg gaacggacaa acctttcgaa tatatcggtt caccatattt accccctacc
     2281 ggtttttctt ttgtgccgaa atcaggatcg ggtgcgaaaa cacctccgtt catgggacaa
     2341 acttgcaatg gatatgatct gtctaaatgg cctgtcgaca gtgtgcgcag cggaaaaata
     2401 aatcttgcat tttttctgga ggtatatcat aattatgcat catcaacgcc attttttctg
     2461 gagaataatt tcttcaataa actggcgggc aatgcagagt tgatgcaaca aataaaagca
     2521 ggattgagcg aagaagaaat tcgcttaagc tggcaagatg atatcaaggc ttttaaacag
     2581 gtgcgcaaaa aatatttact atatcccgat ttcgaataat ggctcatcac ttgcgcatca
     2641 tatacatggg aacgcctgat tttgccgtgc cgggattaca aaaactggtg gaagcaggat
     2701 ttaatgtcgt tgccgttgta actgctcccg ataaaccaag tggtcgcgga ttgcaggtta
     2761 aacctacgcc cgttaaagtg tatgccgatt cgcagggcat tccggtgttg caacccgtga
     2821 aattgaaaga ccctgaattt atctccacat tgcgcactta caatgccgat ctgcaagtgg
     2881 ttgtcgcatt cagaatgtta cccgaagtgg tatggaacat gccgcctaaa ggtaccatca
     2941 acctacacgc atctttatta cctgattatc gcggcgcagc tcccatcaat cacgccatca
     3001 tcaatgggga gaaagaaaca ggtgctacca cattttttct tcaacatgct attgataccg
     3061 gcgatctgat agatcaaatt cgcattccga ttcatgatga tgataatgcc ggcagcatcc
     3121 acgatacact catgatgctc ggtgccgacc ttattgtaaa aacagtgatt gccatcgagg
     3181 aaaataatta ttccacgaaa ccgcaatccg gagaaagtaa taaaatcgcc ccaaaaatat
     3241 tccgcgaaaa ttgtcgcatc gattggaacc aaccggcagc acaaattcat aatgtaatca
     3301 gaggattatc cccttatccc ggcgcattta ccgacatcaa gggtccgcaa tggaaaatcc
     3361 tcaaaagcaa atttgaaata gagccacatc aacaatctcc cggcactatt gcaacagatg
     3421 caaagaccta tttaaagatt gcaagtgccg atggatgggt ttatgtatta gcaatacaac
     3481 ccgaaggcaa acgcaatatg gatatcgcta catttctgca tggatatcgt tcctaagcaa
     3541 taaaaattta aagaaaagga attcagaacc agcgaattct cctaaatacc caatacaagg
     3601 caaagctgat cagaatggag aacagcatga tcacgtagaa tccgctattg gaattctcca
     3661 gtggaagatt cgcgaagttc atgccataga aactcgctat caatgttggc accataagga
     3721 tgatggtgat ggaagtaagg cgcttcatca cgctgttcag attattcgaa atgatagaac
     3781 cgaaggcatc catggtacca ttgaggatgt tggtgtatac cgtcgccatc tcgagggcct
     3841 gactgctgtc gatcactata tcggtgaggt aatcattttt ctcctcatca tttttgatgc
     3901 cgaggaaatt cgtacgctga atcttcatca tcaataattc attggcgcga aggtcagtta
     3961 cgaagtaaac aagtgatttc tggatattga gcaatttcgc caactcggta tttctgctcg
     4021 aatgcatcaa ttctttttcg atgaggtagc ggcgtttgtt catctcattc agatactgaa
     4081 taaaatagaa cacatttttt tcaaatattt tcaataccac caaatcttta tcatgaggcg
     4141 ataaatgttt gatggcaata ctgaaaaacc agtcgattac tttattctgt gccgagctga
     4201 tgatgatatt ataatcctgc gtcaggatga tgccgattgg aatggtgata taaaacgcat
     4261 cgttgtcgat ggagttctcc atatcattct cgataggcgt attgatcacg atgagcttca
     4321 cgttatcatc ctgctcaaat cgcgagcgtt cgttgatatc gatggagtcg agtaaataat
     4381 ctatcgggat gtcgatggcg tcgctgaggg tggcgaggcg tttatggtcg aagggggggt
     4441 atacgtttat ccagcagtct ttttcaggtc tgtcaatctc tacgatctgg ttgtcaattt
     4501 tcttgtagta tttgatcatc tccttccatc tggaacgtat acggaatttt aagtgtgcaa
     4561 agatatggtt tttctttaac aaaatcggca tttctgacct aaaaaatcct ctatgccgga
     4621 gtataactga atagtcgaga tagcttaatg ctgaggtgca cagggaaggt aatagccggg
     4681 agtgctatta aaatcagaag gacaaaatac cgacatcaaa tttgagccaa gcccatattg
     4741 acagcggagg taagtggcat tgattttccc gattcaaacc aggattgaca ctcaggctgg
     4801 gcctcatatg gctgcaacag ttgggatatt gtttgatact gaactgcagc cccaccaata
     4861 acaggatatt gagattgttc gataaaggta tgcagtgctc caaaaatact aaaggcatgt
     4921 ttacggtcgc cccctaaggc caatatatgc acaagcagca tacataaata aaattcgctt
     4981 tccaattcac ctgataattt aacaaattcg gcagactcct tgatcatatt tcgcgcagca
     5041 ataatatctc ccttatttaa ataccctatc gcttgaaatt gctttgaggc agccttaaat
     5101 ccctggtatc ctttttcagg aaaaatcata aacatctcct gtcctatttg tattaattta
     5161 tccgaatcca tttccggttc agatgtggca attgtagctc tgatcaacat cacggcacct
     5221 tgaatatagg tgataccatg ctctttagca atcgaagccg cgagatttaa gtttttatct
     5281 ctgatttcag gttgtccttt ttgacggaaa tattcatcag accaacaata aaacatacag
     5341 ttaattaaag gattattttg cctttccaac aattcaaacc cctttaatat tttctcttcg
     5401 gcgtcttcct tttgcatata cgcgccgtaa catccatcaa aacaatatgc gtaggttaaa
     5461 ttataaatat cattcgtagc ggttaaaata ttttgtgcag cttgcaacat ggcaaaccct
     5521 ctttcaaggt caaatacaaa taacagacaa tacccactcc aggttaatgc gcgaccatgg
     5581 actacaggat caattgtccc gatagtccaa cttgattctt catacaaacc ggcatctttt
     5641 atcagcatca cagtttccgt gaatcccccg cgaatagtcc agtatggcgc taaacaaggg
     5701 aa
//
LOCUS       DAOQHY010000193         5674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_7890_length_5674_cov_5.121196, whole genome shotgun sequence.
ACCESSION   DAOQHY010000193 DAOQHY010000000
VERSION     DAOQHY010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7890_length_5674_cov_5.121196"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PLL28_12705"
     CDS             complement(<1..366)
                     /locus_tag="PLL28_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70231.1"
                     /translation="MNLTLRNLLKVFVLSLGMLSVGNLKAQYCIPDYTIGTGDGDYID
                     GVVLGDISNFSGMGDEYNDYTDLSTLLNPGSTYDLEVYNTPSWPENYAAWIDWNQDEV
                     FSDDERLNPIDLELTAGGSD"
     gene            527..1966
                     /locus_tag="PLL28_12710"
     CDS             527..1966
                     /locus_tag="PLL28_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNF70232.1"
                     /translation="MRPTVLIIDDDPTFCLMLKSFLTKKDFATETAFSGAEALNQVRK
                     QDFDVVLSDFRLPDTDGLELLENIREIKPGTPVIIMTSYADIRIAVKAMKIGAYEYVT
                     KPVNPDEILLIVRNALNREKEKEAMPAAIPASKPQQQSGNKKNPPKNGFEYVEGNSVV
                     AKRIMEYIDVVAPTNLSVIIHGESGTGKEYVAKMIHQKSNRSHKPFVSIDCGTLSKEL
                     AASELFGHIKGSFTGAIVDKTGQFVFAEGGTLFLDEIGNLTYEIQVQLLRAIQERRIR
                     KIGSNQDVEVDVRIIVATNEDLQDAVKKGNFREDLYHRLNEFKIIVPSLRDREDDINI
                     FAEHFLQLANAELNKDVVNFDDEVKAIFRRYSWPGNLRELRNVVRRAVLLTSGEYVRK
                     ETLPHEIVFEKREDVSNNQSVRTENKMQADQPLDLKSLAEKTERELIINTLIKVNYNK
                     SKAAALLKIDRKTLYNKMKLYKILTDDES"
     gene            complement(2004..4598)
                     /locus_tag="PLL28_12715"
     CDS             complement(2004..4598)
                     /locus_tag="PLL28_12715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF70233.1"
                     /translation="MAEQTPNTGLAWKLVLAFTFSMALIGFAAWYAYDSFNKLLGSVE
                     TLSAPDNSDSMLQGLVNSMHAADNDMRNYIMSNDNTSLQSYISTMEHISVELDNLKQD
                     IAFEGQETSLDTLEEYFQDKADLMISLVIFKQSGEYERLNSKAYNKLSTKANESPDIS
                     TIQQNVAIKDPTIKQPAIKQPEVTDATSKKKDKKNKDEGFLRNLFSGKNDKKSDVDAD
                     PTIVALKDTSSNTNNTSNVTIDLAQTSTVDVEDVRRILQEINKEQRRYNTQLSVKELE
                     ILASDKLIIEKIDEVIQRMKIRQQTGIAGQLAIAKDNANASSRTMLIIAAVALLAGLV
                     FMTVILMDIIRSNRYKIQLIEARNRAEYLAKAKEEFLANMSHEIRTPLNAIIGFSEQI
                     EHTPLQPEQKKFIKAVSNAGTHLLNTVNDILDLSKIEAGKLNIDDHTFSIREVIEEVV
                     SILEVKAHEKGLALTTAIGEHADEMVVGDPFRIKQILYNIAGNAIKFTEQGFVEISCS
                     AKKGRKGIVYEIAVKDTGIGIAPDKLLRVFEIFEQAESSITRKYGGTGLGLSISKKLA
                     NIMDGDILVNSKPGEGSEFTLHIPLRLASADEIASVEKDKTEYADLSGLEILLVDDEP
                     YNLMLAEVILNKYGAHITTAANGREALDLIDIKSFDIVLADLHMPEVDGYMLANKIRE
                     RYLQVPLIALTANVMQNDVERIRTSGFNDILLKPYKEVALLQIISKYAPVVFGKQLLE
                     VPEIRESIVEKATMDLFDLSEVMQFADNDPDVLSSIVEAFITNNKQNVELLRDAVHDY
                     NLDNVRNIAHKMLPSYNHFHVYSIVPELKEMEVISPAANGSLVEKFDKVQHVSNLLFA
                     NLRSAITR"
     gene            4741..5424
                     /locus_tag="PLL28_12720"
     CDS             4741..5424
                     /locus_tag="PLL28_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF70234.1"
                     /translation="MANEKIRILIVDDEPDILEVIEYNLLKEDYQVIKAFNGREALEK
                     ARVEKPNLILLDIMMPEMDGIEVCRELRSNHAFDNTLIAFLTARNEDFTQVQGFDVGA
                     DDYITKPIKPRLLVSRIKALLRRTGSADQNHEQVSYGDISIDEEKYLVYVKGKPISLA
                     KKEFELIQLLVSRPGKVFTRQEIFNKIWGMDVIVGDRTIDVHIRKIREKVGEDYIKTI
                     KGIGYKFDQ"
     gene            5439..>5674
                     /locus_tag="PLL28_12725"
     CDS             5439..>5674
                     /locus_tag="PLL28_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70235.1"
                     /translation="MKNASPGRIVLLAALTVFSGILLLEALPLFIPALDKVAYQFIAV
                     PILVFILTYRVLYLAVEKFIYRKIKLIYKNILAN"
BASE COUNT         1713 a         1111 c         1142 g         1708 t
ORIGIN      
        1 atccgatccg cctgcggtta attccaaatc gataggattt aaacgctcat catcactgaa
       61 cacctcgtcc tggttccagt cgatccaggc ggcataattt tcaggccatg atggagtgtt
      121 atacacttca agatcgtaag tagatccggg attaaggagt gtagagagat cagtataatc
      181 attatactca tcacccattc cggagaaatt gctgatatcg ccaaggacaa caccatcaat
      241 ataatcgcca tcgcctgtac caattgtgta gtcgggaata cagtattgcg ctttcaagtt
      301 gccaacagac aacataccca atgaaagaac gaatacttta agcaaattgc gtaaagttaa
      361 attcatagtc gctttttaat atgaaaagaa aggagtctca aatctagcgt acctgcccgc
      421 caaatcaaaa aaaattaaca aacagtctgt ccaccgaaaa tttacatccg tttggtatag
      481 agtctctatc tttaggcgtc ctttaaacac cacccggtat accacaatgc gcccaacagt
      541 cctgattatc gacgacgacc ccactttttg tcttatgctt aaaagctttt tgacaaagaa
      601 ggattttgct actgaaacag cattttccgg tgctgaggca ttgaatcagg tacgcaaaca
      661 ggattttgat gtggtattaa gcgattttcg acttccggat actgatgggc tcgaattgct
      721 tgaaaatatt cgtgaaatca aaccgggaac accggttatt atcatgacat cctatgcaga
      781 tattcgaatt gcggtaaagg caatgaaaat tggcgcatat gaatatgtca caaaaccggt
      841 aaatcccgac gaaattttat tgatcgtcag aaacgcactt aaccgtgaaa aggaaaagga
      901 agccatgccg gcagctattc ctgcaagtaa accacagcaa caatccggca acaaaaagaa
      961 tccgcctaaa aacgggtttg aatatgtgga aggaaatagc gtggttgcca aacgcatcat
     1021 ggaatatatt gatgttgtag ctccaacaaa tttatcggtg atcattcacg gagaaagtgg
     1081 aaccggtaaa gagtatgtag caaaaatgat tcatcaaaag agcaatcgtt cgcataaacc
     1141 atttgtgtcg atcgactgtg gtactttgag taaagaattg gcggcgagtg aattgtttgg
     1201 acatattaaa ggatcattta caggtgctat tgtagataaa accggtcaat ttgtgtttgc
     1261 agaaggtggc actttgtttc ttgatgaaat tggtaatctc acctatgaaa tccaggttca
     1321 attgctgcgt gcaattcagg aacgaaggat aagaaaaata ggtagtaatc aagatgtgga
     1381 agtagatgtg cgcatcatag ttgcaaccaa tgaagatctg caggatgccg ttaaaaaagg
     1441 taatttcagg gaagatcttt atcatagatt aaatgaattt aaaatcattg ttccttcgtt
     1501 gcgcgacagg gaggatgaca ttaatatttt cgctgagcac ttccttcaac ttgcgaatgc
     1561 agaattgaat aaggatgtag ttaatttcga tgatgaagtg aaagctattt tcaggcgcta
     1621 tagctggccg ggaaatttgc gtgagctgcg caatgttgtg cgaagagcag tactccttac
     1681 ttcaggtgag tatgtcagaa aagagacact tcctcatgaa attgttttcg aaaaacgcga
     1741 agatgtttca aataatcaat ctgtccgcac tgaaaataaa atgcaagctg atcagcctct
     1801 ggatctaaaa tcgctggcag aaaaaaccga acgtgagcta attatcaaca ccttgataaa
     1861 agtaaattac aataagagca aggcggcggc attattgaaa attgaccgca aaacattata
     1921 caacaaaatg aagttgtata aaattctaac agacgacgag tcgtaattat ttcaataata
     1981 tctttagcat tcagtcttac agatcatctt gtgatagctg aacgcaaatt ggcaaagagt
     2041 agattgctca cgtgctgcac tttgtcaaat ttctctacca ggcttccatt cgctgcagga
     2101 gagattacct ccatttcttt caattcaggc acaatactgt aaacatggaa gtgattgtag
     2161 gaaggcagca ttttgtgtgc aatatttctt acattatcca gattgtaatc atgcacggca
     2221 tcgcgcaaaa gttcaacatt ttgtttgtta ttggtaataa aagcctctac aatgcttgaa
     2281 agcacatccg gatcattgtc ggcaaattgc atcacttcac tcaaatcaaa taaatccatt
     2341 gtggctttct caactatgga ttccctgatt tcaggaactt ccaacaattg ttttccgaat
     2401 accacgggag catatttcga tataatttgc aacagagcca cttctttata cggcttgagt
     2461 aaaatatcat taaatccact ggttcgaatg cgttcaacat cattctgcat cacatttgca
     2521 gtgagtgcaa tcaatggcac ttgcaaatat ctctcacgaa ttttgttcgc cagcatataa
     2581 ccatcaactt caggcatatg caagtcggca agcactatat cgaaactttt aatatcgata
     2641 agatctaaag cctcgcgtcc attagctgca gtggtaatat gagcgccgta tttattcagg
     2701 ataacctcgg caagcattag attataaggc tcatcatcaa ctaacagaat ttcaagccct
     2761 gacaggtcgg catattcagt tttgtctttt tctacagagg caatttcatc tgcggatgct
     2821 aatcgaagtg gtatatgcaa agtaaattct gagccttcac cgggtttgct gttgacaaga
     2881 atatcgccat ccatgatatt tgccaatttt ttcgaaatag acaatcccaa tcctgtgccg
     2941 ccatattttc tggtgatgga agattcggcc tgttcgaaaa tctcgaatac gcgcaataat
     3001 ttatccggcg caataccaat tccggtatct tttacggcaa tttcataaac aattcccttt
     3061 cttccttttt ttgcagagca actaatttca acaaagcctt gttctgtaaa ctttatcgcg
     3121 ttgcctgcta tattatataa gatttgttta atacggaaag ggtctcccac caccatttca
     3181 tcggcatgtt cgccgattgc tgttgtgagt gctaaacctt tttcatgcgc ttttacttcc
     3241 aaaattgaca ctacttcttc gattacttct ctaatagaaa aagtatgatc atcgatattt
     3301 aacttaccgg cttcaatttt tgataaatca agaatatcat ttaccgtatt caataaatgt
     3361 gtgccggcat ttgatactgc cttgatgaat ttcttttgct ccggttgaag aggagtgtgt
     3421 tctatctgct cagaaaaacc gataatggca tttagcggag tgcgaatttc gtgactcatg
     3481 ttggcaagaa actcctcctt agcctttgcc agatattcag cccggtttct ggcttctatt
     3541 aattgaattt tatacctatt gctgcgtata atatccatca atatcaccgt cataaaaact
     3601 aaccctgcaa gcagagcaac agcagcgatg atcaacatcg tacgtgatga ggcatttgca
     3661 ttatctttag ctattgctaa ctgaccggca ataccggttt gctgacgaat tttcattcgt
     3721 tgaattacct catcaatttt ttcgatgatg agtttatcgc tggccaaaat ttcaagctcc
     3781 ttaactgata gctgagtatt atatctgcgt tgttccttat ttatttcctg aagaattctg
     3841 cgcacatcct cgacatctac tgtcgaagtc tgtgccaggt caatggtgac attggaggtg
     3901 ttattggtgt tggacgatgt atcttttaat gctacaattg ttggatcagc gtcaacgtct
     3961 gatttcttgt cgtttttacc ggaaaataag ttgcggagga atccttcgtc tttattcttc
     4021 ttgtccttct tttttgatgt agcatcagta acctcaggtt gcttgattgc cggctgcttg
     4081 attgtagggt cttttattgc tacattttgt tggatagttg aaatatcagg agattcattt
     4141 gccttcgttg aaagcttatt gtaggcctta ctattaagtc tttcatactc acctgattgc
     4201 ttgaaaataa ccaatgaaat catcaagtcc gctttgtcct gaaagtactc ttccaaggta
     4261 tcgagcgatg tttcctgtcc ttcaaatgca atgtcttgtt ttaagttatc cagttcaaca
     4321 ctgatatgct ccatggtact gatatacgac tgcaagctgg tattatcatt gctcatgata
     4381 tagttacgca tatcgttatc agcggcatgc atgctgttta ccaatccctg tagcatggaa
     4441 tctgaattat ccggcgcgga gagtgtttca actgatccta gcagcttatt gaaggaatca
     4501 taggcatacc aggcagcaaa accgatcagg gccatcgaga aagtgaaggc cagcaccagt
     4561 ttccatgcta atcctgtgtt aggagtttgt tctgccattg cgcacttaaa attaagcgat
     4621 tttgctcata ttgaagcctt tagcccgttc aacgtaaaga aacggtatct ttaacattat
     4681 attaatattg gattcaacat ctattaatat catcatcata gctttacatc aaaatagcat
     4741 atggctaacg agaagattag gattttgatt gtagatgatg agccggatat tctggaagtt
     4801 atcgaataca atctgctgaa agaggattat caggtaataa aggctttcaa cgggagagaa
     4861 gccctggaaa aagcccgggt tgagaagccc aatctcattc tcctggacat catgatgccc
     4921 gaaatggatg gtattgaagt ttgccgtgaa ttaaggagca atcatgcatt tgacaacacc
     4981 ttaatagcct tcttaacggc acgaaacgaa gatttcactc aggtgcaggg tttcgatgtc
     5041 ggagccgacg attatatcac caaaccaatc aagcccagac tgcttgtaag ccgtattaaa
     5101 gccttattgc gacgaacagg gagtgctgat caaaatcatg aacaggtgag ttatggcgat
     5161 atcagtatag atgaagaaaa gtatctggtt tatgtgaagg gaaagcccat ttcattggca
     5221 aaaaaggagt ttgaactcat tcaattgctg gttagtcgcc cggggaaagt ttttacccgt
     5281 caggagatat ttaacaaaat atggggaatg gatgtaatag taggggacag gaccattgat
     5341 gtacatatta gaaagatccg tgagaaggta ggagaggact acattaagac catcaaagga
     5401 attggttata aatttgacca ataattcatt tcatttctat gaaaaatgcc tcaccggggc
     5461 gcattgtttt actggcagca ttgactgtat tttccggcat tctattgttg gaggccttgc
     5521 cattatttat tccggcatta gataaagttg cttatcaatt tattgcagtg ccgattctgg
     5581 tttttatact tacgtatcgc gtactgtacc ttgcggtaga aaaatttatt tacagaaaga
     5641 taaaattgat ttataaaaac atacttgcaa ataa
//
LOCUS       DAOQHY010000194         5644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_7960_length_5644_cov_4.548578, whole genome shotgun sequence.
ACCESSION   DAOQHY010000194 DAOQHY010000000
VERSION     DAOQHY010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7960_length_5644_cov_4.548578"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PLL28_12730"
     CDS             <1..346
                     /locus_tag="PLL28_12730"
                     /inference="COORDINATES: protein motif:HMM:NF012571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70236.1"
                     /translation="ALSYIDNPVAVELISRIYWYTVEFGLIRDNGLLRIYGSGILSSQ
                     GESIYCLKSGIPSKRVDFNVEKILDTPYIKDKFQSIYFVIDSCLQLFESLPDIEQGIA
                     RRIEHPELFVAN"
     gene            603..1292
                     /locus_tag="PLL28_12735"
     CDS             603..1292
                     /locus_tag="PLL28_12735"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNF70237.1"
                     /translation="MLSTLLQIPTVTSTPASTTPPAVDQELHIFELLVKGGPIMIPIA
                     ILLVLAIYLWIYKSITISQLSRMDERMIPAIKDQLTSGNLSSAEAYVRNINTAQARVI
                     DSGIATLGRSMREIESNLETASEIEIMQMEKHLGYMGIIAGIAPMLGFIGTIAGVIKI
                     FYNISVSNDISISIISSGLYEKMITSGFGLLTGIIAYGGYHLLNMKIDRTALKLQRNS
                     FEFIRIINKSE"
     gene            1292..1696
                     /locus_tag="PLL28_12740"
     CDS             1292..1696
                     /locus_tag="PLL28_12740"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNF70238.1"
                     /translation="MKIGRRRKTEAEVFSASMNDIMFFLMLFFLIISTLANPNVIKVM
                     LPNAENVETTYNKQEVSLTVTADKQFFVNDSPVAFEMLESSLISATGGSEDKVVLLRA
                     DATLTIQDLVDVLQIGAKNNLHIVMNTAKSSN"
     gene            1775..3043
                     /locus_tag="PLL28_12745"
     CDS             1775..3043
                     /locus_tag="PLL28_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNF70239.1"
                     /translation="MLFDAAPLAERMRPKSLEEYIGQQHLVGEGQVLRKMAETGRLVS
                     MILWGPPGVGKTTLANILANAADLPFFSLSAVSAGVKDVKEVIEKAQRSGKIVLFIDE
                     IHRFNKAQQDSLLGAVEKGLIILIGATTENPSFEVINALLSRCQVYVLKELERADLQL
                     LVERAVAKDAEISKRKYIIKEDSALLKLSGGDARKLLNIIELIDETVQSSKVEITDAL
                     VTDIVQTKTAQYDKNGEMHYDLISAFIKSMRGSDPNAAMYYLVRMIDGGEDPKFIARR
                     MLILASEDIGNANPNALLLATACFQSVTMLGYPECSIPLAQTVVYLATSPKSNASYMA
                     LMKGYELVRETGDLPVPLAIRNAPTKLMKQLDYGKDYQYAHDHPLNFANMEFLPEEIS
                     GTKLYEPGKNAREEEFRKFLRDRWKEKYGY"
     gene            3284..3553
                     /locus_tag="PLL28_12750"
     CDS             3284..3553
                     /locus_tag="PLL28_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNF70240.1"
                     /translation="MTIKPLADRVIVLPAPAETTTKGGLIIPDTAKEKPQRGVIVAAG
                     PGKKDEPVTVKVGDTVLYGKYAGTEINVDGVEYLIMRESDVFAIL"
     gene            3596..5227
                     /gene="groL"
                     /locus_tag="PLL28_12755"
     CDS             3596..5227
                     /gene="groL"
                     /locus_tag="PLL28_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNF70241.1"
                     /translation="MAKIIKFDVEAREKLKSGVDQLANAVKVTLGPKGRNVVIEKKYG
                     APNVTKDGVSVAKEIELEDPIENMGAQMVKEVASKTSDVAGDGTTTATVLAQAIMAAG
                     LKNVAAGANPMDLKRGIDKAVETVVTELKKMAQNVGNDNKKIEQVAAISANNDAEIGK
                     LIADAMAKVSKDGVITVEEAKGTDTYSEVVEGMQFDRGYLSAYFITNTDEMEAELERA
                     YVLIYDKKISTMKDLLPVLEKVAQQGAQMLIIAEDVDGEALSTLVVNKIRGTIKVCAV
                     KAPGFGDRRKEMLQDIAILTGGTVISEEQGYKLENADLTYLGRAERVTIDKDNTTIVG
                     GKGKKDDIKARINEIKAQIEKTTSDYDREKLQERLAKLSGGVAVLYVGAATEVAMKEK
                     KDRVDDALHATRAAVEEGIVAGGGVAYIRAIEALDKIRGGNEDEKIGIEIIKRSLEAP
                     IRTIVGNAGIEGSIVVQKVREGKADFGFNARTEKYENLLKAGVIDPVKVTRVALENAA
                     SIAGLLLTTECTIVDKPEKKEPMGGGMPGGMDGMM"
BASE COUNT         1704 a         1146 c         1297 g         1497 t
ORIGIN      
        1 agcgctcagc tatatcgaca atccggttgc agttgaattg atctcccgta tttattggta
       61 caccgtagaa ttcggattga tacgcgacaa tggattactg cgtatttacg gctcaggtat
      121 tctttcttca caaggtgaaa gtatttattg cctcaaatca ggcattccat cgaaacgtgt
      181 tgatttcaat gtagaaaaga tattggatac cccttatatc aaggataaat tccaatccat
      241 ctattttgta atcgatagct gcctgcaatt gttcgaaagc cttccggata tcgaacaagg
      301 tatcgcccga cgcattgaac atcctgaatt atttgttgcg aattaatcac ttgatgtagc
      361 ggttttttgc gcttgccggg ttgaagttcc ttcagccccc tgaatgctgt ttatacgaat
      421 ttgaggacaa gaggcgttta tcttgcaact tcgcttattg cctattgagt attattatag
      481 acaattcaat atcagctgcc gcaagcatta tggataattc actttcatcg gcatcctaat
      541 catgtaactt tgttggcata gaattggcat aattctttac attatttaaa gcaactgaac
      601 atatgttaag taccttactg caaataccta ccgtaacatc aactcctgca agcactacac
      661 cacctgctgt tgatcaggaa ttacacattt ttgaattatt ggtgaaaggt ggtcccatca
      721 tgattcctat cgccatttta ttggtgctgg ctatctattt gtggatctat aaatccatta
      781 ccatcagtca actctcacgc atggatgaac gcatgattcc tgccattaag gatcagctta
      841 ccagtggaaa tctttcttcg gcagaagcct atgtgcgcaa catcaatacc gctcaggctc
      901 gtgtgatcga ttcaggtatc gctaccttgg gtcgcagtat gcgagagatt gaatccaatc
      961 tggaaaccgc atctgaaatc gagatcatgc aaatggaaaa acacctcggg tacatgggta
     1021 tcatagccgg tattgcaccg atgctcggat tcatcggaac cattgctggg gttatcaaaa
     1081 tcttttataa tatctctgta tctaacgaca tctccatcag tatcatttct tcaggtctgt
     1141 atgaaaaaat gattacctcc ggatttggtc tgctcacagg tatcatagca tacggtggtt
     1201 atcacttact gaatatgaaa atagatcgca ccgccttgaa attgcagcgt aactcattcg
     1261 aatttattcg catcatcaat aaatctgaat aatgaagatc gggagaagaa gaaaaacgga
     1321 agcagaggtg ttttcggcat ccatgaatga catcatgttc tttttgatgt tgttcttcct
     1381 catcatctcc acactggcga atcccaatgt gatcaaggtg atgctgccga acgccgaaaa
     1441 tgtagaaact acgtacaaca aacaggaggt gtcactcacc gttactgccg ataaacaatt
     1501 ttttgtaaac gacagtcctg ttgcatttga aatgttggaa tcatccctca tcagcgctac
     1561 tggaggttct gaggacaagg tggtattgct tcgtgccgat gcaaccctta ccattcaaga
     1621 tcttgttgat gtattgcaga taggtgcaaa aaataatctg catatcgtaa tgaatactgc
     1681 gaaatcatcg aattaagtaa caccgaaata ctttttatgc gacccggcga attgtcgggt
     1741 cgttttattt tcaggggaat gctagctttg catcatgctt tttgacgcag caccacttgc
     1801 cgaacgcatg cgaccaaaat ctctggaaga gtacattggt cagcagcacc tcgttggtga
     1861 aggtcaggta ttgcgcaaaa tggctgaaac agggcgactt gtcagcatga tattatgggg
     1921 gcctccgggt gtaggaaaaa ctacccttgc aaacattctg gccaatgctg cggatctgcc
     1981 gtttttctcg ctcagcgctg tttctgccgg tgtgaaagac gtaaaagaag tgatagaaaa
     2041 agcacaacgc agcgggaaaa ttgttttatt tatagatgaa attcatcgct tcaacaaagc
     2101 acaacaagat tcattgctcg gtgccgttga aaaaggcctc atcatcctaa ttggtgccac
     2161 cacggaaaat ccatcattcg aagtaattaa tgccttgcta tcgcgatgtc aggtgtatgt
     2221 gttgaaagaa cttgaacgcg ctgatttgca attattagtt gaaagagcag ttgcgaaaga
     2281 tgctgaaatt tctaagcgaa agtatataat aaaagaggat agcgcgcttt tgaaattaag
     2341 cggcggcgat gcaagaaaat tgctcaatat cattgaactc atcgacgaaa cggtgcaaag
     2401 cagtaaagtg gaaatcactg atgctttggt taccgatatt gtgcagacaa aaacagcaca
     2461 atacgataag aatggggaga tgcattatga ccttatttcc gcatttatta aaagtatgcg
     2521 cggaagtgat cccaatgctg ccatgtatta tttggtgcgt atgatcgatg gcggtgaaga
     2581 cccgaaattt attgctcgtc gcatgctcat tcttgcaagt gaagatattg gcaatgccaa
     2641 tccaaatgca ctccttttag caacggcatg ctttcaatcg gttaccatgt tgggttatcc
     2701 tgaatgcagt attccattgg ctcaaacggt tgtttatctt gctacatcac caaagagcaa
     2761 tgccagttat atggcattga tgaaaggcta tgaacttgtc cgcgaaacgg gcgatctgcc
     2821 agtgcctctt gctattcgca atgcacctac caaactcatg aagcaattgg attatggtaa
     2881 agattaccaa tatgcgcatg accatccttt gaatttcgca aacatggagt tccttcccga
     2941 agaaataagc ggcaccaaac tctatgaacc cggcaaaaat gcccgtgaag aagagtttcg
     3001 aaaatttctg cgcgacaggt ggaaggagaa gtatgggtat tgactgaaag ctatgctggt
     3061 aggacgtttc agcctgtttt ccggaaaaaa gaggcaaaaa gtataccaat ttgaagggta
     3121 gggcgtgaca agctgcctgc cgatgaatct gccaaaacct gctattttgt ccctgccaag
     3181 cgtcaaaatt gccgtttcta taccaaggca aggaatttgc tgaaggcgac ctgtcaaatc
     3241 atttaaaatc aaataaatac gtattcatat ggctaaatct aagctgacca tcaaaccact
     3301 tgccgacaga gtaattgtat tgccggcacc tgctgaaact actaccaaag gcggattgat
     3361 cattcccgac actgccaaag agaaaccaca aagaggcgta atcgttgctg ccggtcccgg
     3421 taaaaaagac gagcctgtaa ccgtgaaagt tggcgacacc gtgttatacg gaaaatacgc
     3481 cggcaccgaa atcaatgttg acggtgttga atacctgatt atgcgcgaat cggatgtttt
     3541 cgccattctt tgatctttca aaccttatct gacaaacaac aattaaatat agaacatggc
     3601 taagattata aaatttgatg tggaagcacg cgagaaactg aaaagtggtg tagaccaact
     3661 cgccaatgct gtaaaagtaa ccctcggccc taagggacgc aatgtggtta tcgaaaagaa
     3721 atatggcgct ccaaacgtaa caaaagatgg tgtctctgtt gctaaagaaa tagaactcga
     3781 agaccctatc gaaaacatgg gcgcccaaat ggtgaaagaa gtagccagca aaaccagcga
     3841 tgtagccggt gatggtacta ctactgcaac tgtgcttgct caggctatca tggctgccgg
     3901 tttgaaaaac gttgccgcag gtgctaatcc aatggatctg aaacgcggta tcgacaaagc
     3961 tgttgaaact gtggtaacag aattgaagaa aatggctcaa aatgttggca acgacaataa
     4021 aaaaattgaa caagtagcgg ccatttctgc aaataacgat gctgaaatcg gtaaactgat
     4081 cgctgatgct atggccaaag tttctaaaga tggtgtgatc accgttgaag aagcaaaagg
     4141 aactgatact tactctgaag tagtagaagg tatgcaattc gatcgcggat atttatcagc
     4201 atacttcatc actaacaccg atgaaatgga agctgaactc gaacgcgcct atgtgttgat
     4261 ctatgacaaa aaaatcagca cgatgaaaga ccttcttcct gtgttggaaa aggtagctca
     4321 gcaaggtgca cagatgttaa tcatcgcaga agatgtagat ggtgaagcac tctctacact
     4381 cgtagtaaat aaaattcgcg gaacaattaa agtatgtgca gtaaaagctc cgggcttcgg
     4441 cgatcgtcgc aaggaaatgc tgcaggatat cgccattctt accggtggaa ctgttatcag
     4501 cgaagagcaa ggttataaat tagaaaatgc tgaccttact tacctcggtc gcgcagaacg
     4561 tgtaaccatc gataaagaca atactaccat cgttggcggt aaaggaaaga aagatgatat
     4621 caaagcgcgt atcaatgaaa ttaaagcgca gatagaaaaa actacttctg actacgatcg
     4681 tgaaaaattg caagagcgtc tcgcaaaatt gagcggtggt gttgccgtat tgtatgttgg
     4741 tgctgcaact gaagtagcga tgaaagaaaa gaaagatcgc gtagatgatg cattgcatgc
     4801 tacacgcgct gccgttgaag aaggcatcgt tgcaggtggt ggtgttgcgt atatccgtgc
     4861 aattgaagcg ttggataaaa tccgtggtgg aaacgaagat gaaaaaatcg gtatcgaaat
     4921 catcaaacgt tcactcgaag cgcctattcg tactatcgtt ggtaacgccg gtatcgaagg
     4981 atctatcgtt gtgcaaaaag tacgcgaagg aaaagctgat ttcggattta atgcccgcac
     5041 cgagaaatat gaaaaccttc tcaaagcagg tgttatcgat cctgtgaaag taacacgtgt
     5101 tgcactcgaa aatgcagcct caattgcagg tctgctcctc accaccgaat gcaccatcgt
     5161 agataaacct gaaaagaaag aaccaatggg cggcggaatg cccggaggaa tggatggaat
     5221 gatgtaatgt tatcgtttag tccgcattat acttaggcta attattaaaa gaagagctcc
     5281 gattggggct cttttttttt aggagaaagg agagaggaaa gaggagagag ggggtgagtg
     5341 ggggagtggg tcagtgggtc agtgggtcag tgggtgagtg ggtgagttta cctcgccgtt
     5401 taggcgggcg ggtgagtgcc ctcaccccgg ccctctccca aagggagagg gagtaatttt
     5461 attctaaaac aaagttcaat ctatgcaacg aagttgcaga tctatttggg tctcactact
     5521 cactaccaat cgtgaatcgt gaatcgtgaa tcgtgactcg tgactcgtga atcgtgactc
     5581 gtgaattttt acttgatact tgatacctgt caatgcctga aataagttga ccgtttttaa
     5641 gggc
//
LOCUS       DAOQHY010000195         5628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_7993_length_5628_cov_4.683474, whole genome shotgun sequence.
ACCESSION   DAOQHY010000195 DAOQHY010000000
VERSION     DAOQHY010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5628
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7993_length_5628_cov_4.683474"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /gene="secF"
                     /locus_tag="PLL28_12760"
     CDS             <1..840
                     /gene="secF"
                     /locus_tag="PLL28_12760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNF70242.1"
                     /translation="FKGGRTYTVRFEESIDADKVRNELADFDPGVQVKTFSSSDKLKI
                     TTGYMIENPDPGTDTIVAQELYKQLAGLYHNVDYKTWDSDFKESQMKVEPTIATDIQT
                     SALKAIGLALIAIFIYIVFRFRRWQFGVGAIASLVHDAFMVLAFFSILKNVMPFSLEI
                     NETFIAAILTVIGYSINDTVVVFDRIREYLRESKAGSVQQIFNTAINNTLSRTVITSG
                     TTLLSILVLLIFGNENIQGFAFALFVGIGFGTYSSIFVASAIALDMYKKEDHHKSAEE
                     IMN"
     gene            965..2905
                     /locus_tag="PLL28_12765"
     CDS             965..2905
                     /locus_tag="PLL28_12765"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF70243.1"
                     /translation="MRSIITTILVAICLTASAQPVTEATASNKALKYLNEGVQRGMGK
                     YYDKAIESFQNAIKEEPNFITAWVYLGDTYRMLGNDSMAADTYIKALDIDPDLNSIYF
                     KRLAEAEANIGQYEEAFDHINLFLQDPDVKGSLKTEALHQKNNYNFAKEAIKSPVDFN
                     PENMGPEVNSDFPEYFPSISVDGSVLVMTRRIDDVISLGPGDARPIDNEEFFITYFRD
                     GAWTTSENMGAPINTKLNEGAQCISADGRSLFFTACDNVETGYGSCDIYYSYRIGNQW
                     TAPENCGTIINTGEWESQPSVTADGRELFFSSARFGTVGSYDIWSAKMGDDGYWEEPV
                     NLGNVINTPYSEQCPFIHPDGKTLYFSSDGHPGMGREDLFYSTRDAAGNWSKPKNLGY
                     PINTPNQEISLSVSADGKTAFFSSDRNKAPKDLDIFTFQLPAQVQADPVTWVRATVTD
                     ERTKLPLKANVQLVDIATGNTIVTSTSDPGTGEFLIVLPVGFEYGLYVQREGYLFHSE
                     NFSLLNVLPDAPFDINVALQPISQGEILTLKNIFFETASAEILPISEPELNKVVDLMK
                     KNPGMQVRVNGHTDNIGTEIDNQKLSQNRANSVKAYLVSKGIEDARISTMGFGESKPI
                     DTNANEVGRANNRRTEIEILRL"
     gene            2996..3877
                     /locus_tag="PLL28_12770"
     CDS             2996..3877
                     /locus_tag="PLL28_12770"
                     /inference="COORDINATES: protein motif:HMM:NF025007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HNF70244.1"
                     /translation="MPIDISTPIPVIDGEKITREEFERNYVHPQKPVILRGLWKNYPA
                     YHKWNYNFFRKSMGDLEVGLYSSKQADPSQTLSTPTTKMKFSEYLKLIETEPTDLRLF
                     LFPIFKHKPELLHDFDYPPITKGYIKLPFMFFGPKNSITRMHQDIDMSNVFLTQFEGR
                     KRVVLFHPNQSDLLYKLPFNVHSTVDIEHPDYDTYPGLKYVHGMEGILEYGDTLYMPA
                     GYWHHIEYVEGGYGLSVRTLAPTLALRLRGAYNLLIQRKLDDILRFLFGKRWFELKKR
                     IAHDRANRAIEKILRDE"
     gene            3986..4738
                     /locus_tag="PLL28_12775"
     CDS             3986..4738
                     /locus_tag="PLL28_12775"
                     /inference="COORDINATES: protein motif:HMM:NF017551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HNF70245.1"
                     /translation="MCLISFAFQSHPEFPLILIGNRDEFYARPTANLHQWENTTIPVY
                     GGRDLKEGGTWMGMNTQGSFAALTNYRDPAHMADDKPSRGNLLRDFLSGDIPLEEFHR
                     LLRSRGTAYNGFNIIYGNCEELYYYNNIQQQLRQLYPGIYGLSNAFLDTPWPKVLKAK
                     DALQRNLHISNNEDQLIHAFTDISEADDAALPSTGVSPEWEKKLSAMFITSPSYGTRL
                     TTFVSIDNRRNVVYREKGYVPEADTRFTFNIQ"
     gene            4751..>5628
                     /locus_tag="PLL28_12780"
     CDS             4751..>5628
                     /locus_tag="PLL28_12780"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNF70246.1"
                     /translation="MRNCFRKYGCIWVLLVCLNCMHVNKASGQQYFLKNYSLSDGLAG
                     ISVSSIIQDSRGYIWIGTMDGGLSRFDGKTFVNYTKHDGIGDNAITCLYEDRSGNIWI
                     GTENNGVTKFNGFEFEHFDDPGINAVDKIYSDTSGNILIYTFPDLYKIKGDSIILSSK
                     RNSEYGLQNFLLAGGPKAVKTIIDKRGNKWVASHAGISFVPKSFVDTDDQYAHAEHFI
                     LNPENPEEPASCIMQDREGNIWIGTPLSGAFMFYDGAFSNFNNLPSLRNTYITAFTTA
                     NNALIIGTPTGIKEFI"
BASE COUNT         1697 a         1259 c         1160 g         1512 t
ORIGIN      
        1 ttcaaaggcg gtcgcacata tacagtgcgt ttcgaggaat caatcgatgc tgataaagtg
       61 cgcaatgaac tcgctgattt cgatccgggt gtacaagtga aaactttcag tagctccgat
      121 aaactgaaaa tcacaaccgg ttatatgata gaaaatcctg atccgggaac tgatactatt
      181 gttgctcaag aattatacaa acaattggct ggattatatc acaacgttga ttataaaacc
      241 tgggatagcg atttcaaaga aagccaaatg aaagttgaac ctaccattgc gacagatatt
      301 cagacatctg ccttgaaagc aatcgggttg gcactcatcg ctatctttat ttatatcgta
      361 ttccgattcc gcagatggca atttggtgtt ggtgctatcg catcgctggt gcatgatgca
      421 tttatggtgc tggcattctt ctccatcctt aaaaatgtaa tgccgttctc acttgaaata
      481 aatgaaacgt tcatcgcagc aattctgacg gtgattggtt actccatcaa cgatacggtg
      541 gtggtattcg accgtattcg tgagtatctg agagaaagta aggccggttc agtgcaacaa
      601 attttcaata ctgcgataaa taatacatta agtcgtaccg taattacctc aggtacaact
      661 ttattgtcta tcttagtatt attgatattt ggaaacgaaa acattcaagg ttttgccttc
      721 gcattgtttg tgggtatcgg ttttggtacc tattcatcta tcttcgtagc ttctgcaatc
      781 gcattagata tgtataaaaa agaggatcat cacaaatctg ctgaagaaat aatgaattaa
      841 gcacaccact ctgactaagc gaaaaacccc tgcttcccac aggggttttt cttttgtatg
      901 catgtgcgga acaatttttg tttacatatc agtaaacacg catctttacg tttataatac
      961 tgtaatgaga tctatcatca ccaccatact agtggctata tgccttactg caagtgcaca
     1021 gcctgttact gaggctacag cctccaataa ggctctgaaa taccttaacg aaggcgttca
     1081 aagaggaatg ggcaaatatt acgataaagc catcgagagc tttcaaaacg ctatcaaaga
     1141 agagccaaac ttcatcacgg cttgggttta ccttggcgat acctaccgta tgttaggaaa
     1201 tgattccatg gcggctgata cctatatcaa agcactggat atcgatcctg atctgaatag
     1261 catctatttc aaacggctgg cagaggctga agctaatatc ggccaatatg aggaagcatt
     1321 cgatcatatc aatctattcc tgcaagatcc tgatgtgaaa ggctcactca aaaccgaggc
     1381 attacatcag aaaaacaatt acaactttgc taaggaagcg atcaaatctc ctgtcgactt
     1441 caaccctgaa aacatgggcc cggaggtaaa ctctgatttt cctgaatact tcccatccat
     1501 ttctgttgat ggcagcgtgc tcgtgatgac ccggcgtatt gatgacgtga tcagcctcgg
     1561 acccggagat gccagaccta tcgataacga agaattcttt atcacttatt tccgtgatgg
     1621 cgcatggact acctcggaaa atatgggagc gcccatcaat accaagctaa atgaaggcgc
     1681 acaatgtatc tctgcagacg gtagatccct gttttttaca gcttgcgata atgtagaaac
     1741 cggttatggt agctgcgaca tttactattc atatcgcatt ggcaatcaat ggactgcacc
     1801 tgagaactgc ggcactatca tcaataccgg tgaatgggaa tctcaacctt cagtgacagc
     1861 cgatggtcgc gaattgttct tcagctcggc acgctttggc accgttggaa gctacgatat
     1921 ctggtcggca aaaatgggtg atgacggcta ttgggaagaa ccggtgaacc ttggaaatgt
     1981 aatcaatacc ccatactcag aacaatgccc gtttattcac cccgacggca aaacattgta
     2041 tttctcgagc gatggtcacc cgggcatggg cagagaagac ctcttctact ccacacgtga
     2101 cgcagcagga aactggtcga aacctaaaaa tctcggctac ccgattaata ccccgaatca
     2161 agaaatcagc ctaagtgtat ctgccgatgg caagacggca tttttctcta gcgataggaa
     2221 taaggcacct aaagaccttg atatcttcac attccagctc cctgcgcagg tacaggcaga
     2281 tccagtaaca tgggtccgcg caacggtgac agatgaacgc accaaattac cgctcaaagc
     2341 taatgtgcaa ttggttgaca tcgctaccgg aaatactatt gttacctcta cctccgatcc
     2401 cggcaccggt gaattcctta tcgtgttacc tgtcggattc gaatatggcc tgtatgtgca
     2461 acgagaaggg tatttattcc actccgaaaa ctttagcctc ctcaatgtgt tgcccgatgc
     2521 cccattcgat attaatgtgg cgttacaacc tatcagtcag ggagagattc tcaccctgaa
     2581 aaatattttc ttcgaaacgg catccgctga aatcctccct atatctgagc ctgagctgaa
     2641 taaagtggtt gatctgatga agaaaaatcc cggtatgcag gttcgtgtaa atggacatac
     2701 agataatatc ggtacggaaa ttgataatca aaaattatca cagaacaggg caaacagtgt
     2761 gaaagcatac ctcgtaagca agggtattga agacgcacgt atcagcacca tgggttttgg
     2821 tgaatctaaa cccatcgata ccaatgccaa cgaagtaggt cgcgctaata accgacgcac
     2881 tgaaattgaa atcctgcgct tgtgaggtgc acgtgtaatt taaatgaatt taacaccggc
     2941 gatgaaacaa aggatttaaa ttcactgtta atgatacact ataaaacctt gctatatgcc
     3001 gatcgatatc tcaacaccaa ttccggtgat cgatggtgaa aagatcactc gtgaagaatt
     3061 cgaacgcaac tatgttcacc cgcaaaaacc ggtgatcctt agaggactct ggaaaaatta
     3121 tccggcttac cacaaatgga actacaactt ctttagaaaa tccatgggcg atttggaagt
     3181 cggcctctac agcagcaaac aagctgatcc gagccaaaca ctttcaacgc cgacaacaaa
     3241 aatgaaattc agcgaatacc tgaaactcat tgaaacggag cctaccgatt tgcgcttatt
     3301 cctcttccct attttcaaac acaaacccga gttacttcac gactttgact atcctccaat
     3361 cactaaagga tatatcaaat taccattcat gttctttggt ccgaaaaaca gcatcacacg
     3421 catgcatcag gacatcgata tgagcaatgt gtttcttaca caatttgaag gaagaaaacg
     3481 cgtggtgcta tttcatccca atcagtcaga cctgttgtac aagctccctt tcaatgtgca
     3541 ttctacagta gatatcgaac atcctgatta tgatacttat ccgggattaa aatatgtgca
     3601 tggcatggaa ggcatacttg aatatggcga tacattatac atgcctgccg gttattggca
     3661 tcatatcgaa tatgttgaag gcggatatgg attgagtgtt cgcaccttag cccccactct
     3721 tgcccttcga ttgcgcggtg catataattt gttgatacaa cgcaaactcg acgacatcct
     3781 tcgtttcctt tttggaaaac gttggttcga attgaaaaag cgtatagccc acgatcgtgc
     3841 aaaccgtgcc attgaaaaaa tattgcggga tgaataggaa aatcacaatc aacacaatcc
     3901 ggcattgtat tgatgataat taggcgatgt gaacgattcc gtcctgaaga tatgttgatg
     3961 caacataaaa cactatcttc atgacatgtg tctgatcagt tttgccttcc aatcgcatcc
     4021 tgaatttccg cttattttaa tcggcaacag agatgaattt tatgctcgcc ctactgcgaa
     4081 tttacaccag tgggaaaata ccacaattcc cgtttatggc ggtcgcgatt tgaaggaagg
     4141 aggcacctgg atgggcatga atactcaagg ctcatttgca gcgcttacca actatcgcga
     4201 tcccgctcac atggccgacg ataagccttc gcgcggcaac cttttgcgtg attttttgag
     4261 cggcgatata ccattggagg aatttcatcg actattgcga agcagaggga cagcttacaa
     4321 cggttttaat atcatctacg gcaattgcga agagttatat tattacaata atatccaaca
     4381 acaattgcgt caattatatc ccggtattta tggtttgagc aacgcctttc ttgatactcc
     4441 atggccaaaa gtgctcaagg ctaaggacgc cctgcaaagg aatctgcata tatccaataa
     4501 cgaagatcaa ttgatacatg catttaccga catttcagaa gccgatgatg ctgccctacc
     4561 ctcaacggga gtaagccctg aatgggagaa aaaactgtcg gccatgttca taacttcgcc
     4621 ctcctacggt acccgattaa ctacatttgt aagtatcgat aaccggcgaa atgtggtata
     4681 tcgcgaaaaa ggatacgttc ctgaagctga tacacgtttc acctttaata ttcaataaga
     4741 tatctgctac atgcgcaatt gtttccggaa atatggctgt atctgggtgt tgttggtatg
     4801 cctcaactgc atgcatgtaa acaaagcatc cggccagcag tatttcctta aaaattatag
     4861 cctgagcgat ggtttagcag ggatttccgt ttcaagtatc atccaggatt caagaggata
     4921 tatctggatt ggaaccatgg acggcggatt atcccgcttc gacggaaaaa cgtttgttaa
     4981 ctataccaaa cacgatggta tcggcgataa tgccatcacc tgcctgtacg aagaccgcag
     5041 cgggaatata tggataggaa cagaaaataa tggcgtcaca aaattcaatg gttttgaatt
     5101 cgaacacttc gatgatcccg gtattaacgc tgtcgacaaa atctatagcg acacctccgg
     5161 taatatcctc atttatacgt tccctgatct ctacaagatt aaaggcgaca gtattatcct
     5221 ttcaagcaaa cgcaatagcg aatacggact gcaaaatttc ctgctagcgg gcggacctaa
     5281 agccgtaaaa acaattatag acaaacgagg caataaatgg gtagcaagcc acgcaggcat
     5341 ttcctttgtg ccgaaatcat ttgttgatac agatgatcaa tatgcacatg cagaacattt
     5401 tatcctcaat cctgaaaatc ctgaagaacc ggcatcatgt atcatgcagg atcgtgaagg
     5461 gaatatttgg ataggcactc ctttgagcgg tgcatttatg ttttacgatg gtgcattttc
     5521 aaatttcaat aatcttcctt cacttcgcaa tacatacatc actgcattca ctactgccaa
     5581 caatgccttg atcattggca caccaacagg aataaaagaa tttattta
//
LOCUS       DAOQHY010000196         5589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_8076_length_5589_cov_4.968377, whole genome shotgun sequence.
ACCESSION   DAOQHY010000196 DAOQHY010000000
VERSION     DAOQHY010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8076_length_5589_cov_4.968377"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..1947)
                     /locus_tag="PLL28_12785"
     CDS             complement(214..1947)
                     /locus_tag="PLL28_12785"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNF70247.1"
                     /translation="MNKFAELGLSSRIVEGIEVLGFTEPTPIQAQVIPAVLNEPTDLI
                     ALAQTGTGKTAAFGLPLVEMIDVTARIPLALILAPTRELCVQIENDLRNFSKTTKGFQ
                     TVAVYGGASIRTQIEQIRRGVQVVVATPGRLIDLITRKALNLSDVQVVVLDEADEMLN
                     MGFMEDIDTILAETPEDKQTWLFSATMPTEIRQIIKKYMSNPVEISSGGGNKTNENIE
                     HQYYMVEARNKYPALKRILDFHPDIYGIIFCRTKAETQDMAERLIKDGYNADSLHGDL
                     TQMQRDKVMNAFREKTLQVLIATDVAARGIDVDNLTHVLHLHLPDDMAYYTHRAGRTA
                     RAGKTGVSCVLVTSKDLYRIKQLERTLKIDFKKMMVPDGMAICEKQLLNLLHRVNEVK
                     VDSAAIARFMPVMEREFAGMDRDEIMMRFASLEFNRFLDYYRFAEDLNVYERRETRDQ
                     AARKGNMDLLFINLGKMDHIGPKDLLVMICQVGKLKAHQIGDIRLKGAYSFIEVPANA
                     SQTIIQAFNGFIYRGRRVRLEIRNDKEESFSEKRRSHKGSSREMPRKEHGSGFKKDGG
                     FKKEHRKGKKG"
     gene            2191..4311
                     /locus_tag="PLL28_12790"
     CDS             2191..4311
                     /locus_tag="PLL28_12790"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HNF70248.1"
                     /translation="MRKFILLLIVLGFVGFQAQAQVNRCGVMEHEAVLQAKYPDYATK
                     QAAREQMTQKIIQKMQANGKMEGGATIVTIPVVFHVVYKTAAQNIPDARLLEQIDILN
                     EDFRRLNDDAANTPAAFTGVAADFEIEFCLASIDPNGNPTTGINRIETTVSSFGTDDN
                     VKFAADGGADAWPADSYLNFWVCNLGASLLGYAQFPGGPANTDGVVIHYNHVGNNAAG
                     YPYHLGRTATHEIGHWLDLRHIWGDDTNCAGSDLCGDTPNQKVETYGCPGYPKTDACS
                     ATSPGIMFMNYMDYTDDDCMNMFTEDQKSRARALFEPGGDRYGLLFSEGCGLQPFDAQ
                     AISSLPGGTVCNLSFTPAVEIKNHGTETLTSIDIIYSVDGGATSTFGWTGSIVTNATE
                     LVYLPSLTTTEGEHTLEVTLENPNGNTDADASDNITSTDFLVNLGSSMLPMVQGFEAS
                     GFPYTDYTLYNPDGGEGWERTNFGASIGTYSIFLNHFNNSNFGDIDEFVLPAYDMSDL
                     DEVHFTFDVAYALYTASGVYSDTLEVLVSDDCGDSWTTLYKKANPDLQTAPPHTITFK
                     PEDDEWRQESIDLSAYLGQPQVFVKFRTITNYENNLYVDNININDGTLPQAIDDLAND
                     FALSLFPNPTSNNITVRFNTPIAGAYSVIVYNVTGALVYTSNTNASNGVNLISVPAEG
                     LGAGLYHVQVSNGVLVSGDYFIKE"
     gene            complement(4358..5020)
                     /locus_tag="PLL28_12795"
     CDS             complement(4358..5020)
                     /locus_tag="PLL28_12795"
                     /inference="COORDINATES: protein motif:HMM:NF036823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNF70249.1"
                     /translation="MALILREHYQHLAEYAVWKIEEEPEFYLKGLILSEWEQNYLNRI
                     NHPKRRLTWLASRYLLKLLLNTSAFVELLFDEHGKPYVANDIVKVSISHCDTYAAAMV
                     SRECGVGIDIETPERDISPIQNKFLSPTELEQINTGDKRELLMVYWGAKEVIYKIYGK
                     RKLEFKDDMYIKPFILQQRGDIDGMLMKNGLVADYLMHYFQNDRFILVVGVEKEVSIL
                     NQ"
     gene            5083..5346
                     /locus_tag="PLL28_12800"
     CDS             5083..5346
                     /locus_tag="PLL28_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70250.1"
                     /translation="MHATRSIFGMQNHTMFKFFQFVRAEQEFLHIEVPKHLKGKLVEI
                     ILQEKAPDPDTQLAPDEEQQMAQKEAELKMLDDMRNQLNVWDT"
BASE COUNT         1486 a         1293 c         1258 g         1552 t
ORIGIN      
        1 gatagagagg gagctgattc cgcttcggaa ttaaaatatt gcaaagcaat ataattgcat
       61 ttcctattaa cgcgccggcg ttaaaagcac ccacacttgc taacttgcta accttcaaac
      121 ttgctcacta agacgagcgt cttcatgcaa ccccccccca tcggctaatt ggctaatctg
      181 ctaattggct aattagctaa ttagctaatc acctcatccc ttcttcccct tcctatgttc
      241 cttcttaaac ccgccgtctt ttttgaaacc ggagccatgc tcttttcggg gcatttcacg
      301 ggacgaaccc ttgtgtgaac ggcgtttttc actgaagctt tcttctttat cgttgcggat
      361 ctcaagtctt acacggcgac cacgataaat aaatccgttg aaagcctgaa taatggtttg
      421 agatgcatta gcagggactt caatgaatga atatgcacct ttcaggcgga tatcaccaat
      481 ttggtgggct ttgagcttgc caacctgaca aatcataacc aacaagtctt tcgggccgat
      541 atgatccatt ttacccaggt tgataaacaa caagtccata ttgcccttgc gagcagcctg
      601 atcgcgggtt tcgcggcgtt cgtaaacgtt caggtcttca gcaaagcggt aataatcaag
      661 gaaacggttg aattctagac tggcaaaccg catcatgatt tcatcgcggt ccattcccgc
      721 aaactcacgc tccatcaccg gcatgaatct ggctattgct gctgagtcta ccttaacttc
      781 gtttaccctg tgcaacaggt ttaggagctg tttttcgcag atagccatgc cgtctggcac
      841 catcatcttc ttaaagtcga ttttcagggt gcgttcaagt tgctttatgc gatagaggtc
      901 tttagaagta accaacacgc aagaaacccc tgttttcccg gcccttgccg ttcttccggc
      961 acgatgagtg taatatgcca tatcgtcggg gagatgcagg tgcagcacgt gtgtcagatt
     1021 atccacatca atacctctgg cagcaacatc cgtagctatg agcacctgaa gtgtcttttc
     1081 acggaaggcg ttcatcacct tatcgcgttg catttgggta aggtcgccat gcaaagaatc
     1141 ggcattataa ccgtccttga taaggcgttc ggccatgtct tgcgtttctg ctttcgtgcg
     1201 acaaaaaatg attccgtaaa tgtcgggatg aaaatcgagg atgcgtttta aggcaggata
     1261 tttattcctc gcctcgacca tataatattg atgctcaatg ttttcattag tcttatttcc
     1321 accaccggat gagatctcta ccggattgct catgtatttc ttgatgatct ggcggatctc
     1381 tgtaggcatg gttgcagaga acaaccaggt ctgcttgtct tcgggagttt cggcaagaat
     1441 ggtatcaata tcttccatga aacccatatt caacatctca tcagcctcat caagcactac
     1501 aacttgcaca tcgctgaggt tgagcgcctt gcgggtgatg aggtcgatca atcggcctgg
     1561 agttgccact accacctgca caccgcgtcg aatctgctcg atttgtgttc tgatacttgc
     1621 gccgccatac actgcaacag tctgaaaacc tttggttgtt ttagagaagt tgcgcaggtc
     1681 gttttcaatc tgcacacaca attcgcgtgt aggtgccaaa atgagcgcca acggaatgcg
     1741 ggcggtaaca tctatcattt ctaccaatgg taatccaaag gcagcagttt tacctgtgcc
     1801 agtttgtgcc agggcgataa ggtctgttgg ttcatttaac acggcgggga tcacctgtgc
     1861 ctgtatggga gtaggttcgg taaatcccaa tacctctatc ccttccacaa tgcgtgatga
     1921 aaggcctaat tcggcaaatt tattcatata atttcttgta attcaaatat ttatatactg
     1981 ttatctacta caccacatta tgcctgttca aagcgctcgt ggggctaatt ttgaaaaata
     2041 ccgtgagcag atacaaggag gaatgcgctg caaagatact gaaaatagcg ctgtttcagc
     2101 tattcgccaa gttgtggaag gaaagtgcgt aaaacggcgt aaacaacaag caggaaaccc
     2161 ttacctttaa ggcttattct aatctacatt atgcgtaagt ttattttact ccttatcgtg
     2221 ctgggttttg ttggctttca ggctcaggca caagtaaatc gttgcggggt tatggaacac
     2281 gaagcagtgt tacaggctaa ataccctgat tatgcgacaa aacaggctgc cagagaacag
     2341 atgacccaaa agatcatcca gaagatgcag gctaatggca aaatggaagg tggtgctact
     2401 atagtgacca ttccggtggt atttcatgtt gtttacaaaa ctgccgctca gaacattccg
     2461 gatgcacgac ttttggaaca aattgacatt ctaaatgagg atttccgtcg cttaaatgat
     2521 gatgcagcca atacaccggc agcctttaca ggagtcgctg ccgattttga aattgaattt
     2581 tgcctcgctt ccatagatcc aaatggcaat cccaccacgg gaataaacag gattgaaact
     2641 actgtgtctt cattcggcac agatgataat gtgaaatttg ctgctgatgg tggcgctgat
     2701 gcatggcctg ccgacagcta tttgaacttc tgggtgtgta accttggcgc ttcgttgttg
     2761 ggttatgcac aatttccggg aggaccggcc aacactgatg gtgtggtaat acattacaat
     2821 cacgttggaa acaatgctgc gggttatcca taccatttag gtcgcaccgc tactcacgaa
     2881 attggccact ggcttgacct tcgccatatc tggggtgatg atacaaactg cgctggatct
     2941 gatctttgcg gtgatacgcc aaatcaaaag gtagaaactt acggttgtcc gggttaccct
     3001 aaaaccgatg cttgttctgc tacttcaccc ggcatcatgt tcatgaacta catggactat
     3061 acagatgatg attgtatgaa tatgttcact gaagaccaaa aatcacgcgc ccgcgcatta
     3121 tttgaaccgg gtggcgatcg ctacggattg ctgttttcag aaggttgtgg cttgcagcca
     3181 ttcgatgcac aggccatttc ttcgcttccg ggcggaaccg tttgtaactt atcatttacg
     3241 cctgcagtcg aaattaagaa ccatggcaca gaaacactca catcaataga tatcatttat
     3301 agcgttgacg gcggtgctac ctcaacattc ggctggacag gatccatcgt aaccaatgct
     3361 actgaattgg tgtatttgcc ttcgttaacc actactgagg gcgaacatac ccttgaggta
     3421 acgcttgaaa atccaaacgg caatactgat gctgacgcca gtgataatat tacctctacg
     3481 gatttcctgg ttaacctggg atcttccatg ttacctatgg tgcagggatt tgaagcttca
     3541 ggcttccctt ataccgacta taccctttac aaccccgacg gtggtgaagg atgggaaaga
     3601 acgaatttcg gcgccagtat tggcacatat tccatattcc tgaaccactt caataactcc
     3661 aattttggtg atatcgatga gtttgtactt ccggcttacg atatgagcga tttggatgaa
     3721 gtgcatttca cctttgatgt ggcctatgcg ctgtatacag ccagcggcgt atactccgac
     3781 actctggagg tattagtaag cgacgactgc ggcgattctt ggactacact ttataagaaa
     3841 gccaatcctg atttgcaaac agcaccacct cataccataa cattcaaacc ggaggatgac
     3901 gagtggcgtc aggaatcaat tgacctgagt gcatatctcg gtcagcctca ggtgtttgtg
     3961 aaattccgca ctatcacaaa ttacgagaat aacttgtacg tggataatat caatatcaat
     4021 gatggcacgt tacctcaggc tattgacgat ttggccaacg atttcgcttt gagtctcttc
     4081 ccaaatccaa cgtcaaacaa tataacggtg cgtttcaaca ccccgattgc aggggcttac
     4141 agcgtgattg tgtacaatgt tacgggagct ttggtttata catcaaacac aaatgcttct
     4201 aacggtgtaa acctgatcag cgttcctgcc gaaggtcttg gagcaggctt gtaccatgtg
     4261 caggtaagca atggcgtcct cgtttcaggt gattatttca taaaagaata agcaaacctt
     4321 aaccaattta tagaaggcct ccgatcgggg gcctttttta ttgattgagg atggagactt
     4381 ccttctcaac gcctacaacc agaatgaatc tgtcattttg gaagtagtgc atcaggtaat
     4441 cagcaaccag tccgtttttc ataagcattc catcaatatc gcccctttgt tgcagtatga
     4501 aaggcttgat gtacatatcg tccttgaatt ccagtttccg cttaccatag atcttataaa
     4561 tcacttcctt ggcgccccaa taaaccatga gaagctctct cttatcgccg gtattgatct
     4621 gttccagctc ggttggcgat aagaatttgt tttggatggg tgaaatgtcg cgctcgggcg
     4681 tttcaatatc gatgccaaca ccacactccc tcgacaccat agctgcggca taggtatcgc
     4741 agtgactgat actcactttt acgatatcgt ttgcaacgta aggtttaccg tgttcatcga
     4801 aaagtagttc aacaaaggcg gaggtattca acagaagctt aagcagatag cggcttgcaa
     4861 gccaggtaag acggcgcttt gggtggttga tgcggttgag atagttttgt tcccattcgc
     4921 tgagtatgag gccctttaag tagaattcag gctcctcctc tatcttccag acagcatatt
     4981 cggccagatg ctgataatgt tcccgcaaaa taagtgccat gctaaactat tcacaggcga
     5041 aattaggcta attaaacctc ggcagacaaa aaatatttgc acatgcatgc aacccgcagt
     5101 atcttcggga tgcaaaacca caccatgttt aaatttttcc agtttgttcg cgccgagcag
     5161 gaattcctgc atatagaggt tcccaagcac ctgaaaggca agctggtaga gattattctg
     5221 caagaaaaag cccccgatcc cgacacccaa ctcgcccccg acgaagaaca gcaaatggcg
     5281 caaaaagaag cagaactcaa aatgcttgac gatatgcgca atcagttgaa tgtttgggat
     5341 acgtgaggtg atgagttgat tagccaatta gcagattagc caattagccg atgggggggt
     5401 gcttgcatga agacgctcgc attagtgagc aagtttgagg gttagcaagt tagcaagtgt
     5461 agttaattag caaattagca agttagcaag tgtgtgggtg cttttaacgc tggcgcgtta
     5521 atagggaatg caaatatatt gctttgcaat attttaattc cgaagcgcaa tcagctccct
     5581 ctctatcga
//
LOCUS       DAOQHY010000197         5589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_8077_length_5589_cov_4.670763, whole genome shotgun sequence.
ACCESSION   DAOQHY010000197 DAOQHY010000000
VERSION     DAOQHY010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8077_length_5589_cov_4.670763"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            316..1368
                     /gene="pheS"
                     /locus_tag="PLL28_12805"
     CDS             316..1368
                     /gene="pheS"
                     /locus_tag="PLL28_12805"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNF70251.1"
                     /translation="MDAKGDQFDHIKEIQEDINSFPVTDAASLEQFRIRFLGSKNILK
                     DVFSAIKDVPNERKKEFGQLVNALKQSAEQRFEILEAQYKKQSDKSADQVDVSRPAIT
                     NAPGSLHPVTLIKDELIGIFTRMGYTVVDGPEIEDDWHNFSALGMPEDHPARDMQDTF
                     YISTNPAVLLRTHTSNVQIRVMEELAKSGKQPPVRIVALGRTYRNETISARAHCTFNQ
                     IELLYIDEGVTFADMKQTLLYLAQELYGENTTIRLRPSYFPFTEPSAEMDVSCFVCGG
                     DGCAICKHSGWVEIGGCGMVDPVVLENCGIDSEKYTGFAFGFGLERIAILKYKINDIR
                     LFTENDLRFLKQFEQL"
     gene            1377..1982
                     /locus_tag="PLL28_12810"
     CDS             1377..1982
                     /locus_tag="PLL28_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70252.1"
                     /translation="MKIILSLFAFICLLQVDMHAQNTTLGADTSKITKDSIIMTIVGE
                     DTLIGPPITFRKYNERFVDLMFFDGNYNIVSFHIAEGSTSFQLMLSSLDSATYCFSVS
                     PDEFKMLMAYMKQFNDELLSQPNYPVKCNYCQTIQVFWYDGDKEKSTLKRSYRIPQVF
                     PEILDFCYDLSDDKRRCKISLDNFMSLEGFEDNLRGPASQK"
     gene            1987..>2435
                     /locus_tag="PLL28_12815"
     CDS             1987..>2435
                     /locus_tag="PLL28_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNF70253.1"
                     /translation="MPFSYTDSLNYVSKLTPRRVWNMTKILGSYYYSMWTKKPVHKGN
                     PISISFEPTTSCNLRCPECPSGLRAFTRPTGMLNQDFFRDTIDAIHKDLTYLIFYFQG
                     EPYLNPAFLDMVAYANKKKIYTATSTNAHYLNDENARKTIESGLDRL"
     assembly_gap    2436..2535
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2536..3164
                     /locus_tag="PLL28_12820"
     CDS             <2536..3164
                     /locus_tag="PLL28_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNF70254.1"
                     /translation="LNDENARKTIESGLDRLIISIDGTTQDVYQQYRVGGNLEKVLEG
                     ARNIVKWKKEMRSKTPFVIFQFLVVKPNEHQIEDVKKLGKEIGVDEVAFKTAQIYDYE
                     NGNDLIPTIDYYSRYAKQDDGKYRIKNKMLDHCWKMWHSCVITWDGAVVPCCFDKDAE
                     HKLGDLKQQTFSELWRGPAYQHLRSSLLRSRSEIDICKNCTEGTKVWA"
     gene            3172..4350
                     /locus_tag="PLL28_12825"
     CDS             3172..4350
                     /locus_tag="PLL28_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNF70255.1"
                     /translation="MHHRQLFLRHVAQTSDAPLAIEITQAAGNWLWDADGKKYLDLIS
                     GIAVSNIGHGHSAVKSAINDQVDKYMHVMVYGEIVQSPQVQYATWLTNHLPSTLNSVY
                     FVNSGSEAVEGAIKLAKRVTGKTEIISFRNSYHGSTMGALSVGNTEERKNAFRPLIPD
                     NTVLTYNDPAAIQAISSRTAAVIIEPIQAEAGVITPNSEFLQQLREACSTHDAMFILD
                     ECQTGFGRTGTLFAFEQMNLVPDMLVLGKAVGGGMPLGCFIADIASMQAFTHDPVLGH
                     ITTFGGHPVCCAAGLAAAQVLEDEKLMEHIPNLLQVFTSKLQHPKIKSFRHAGLLMAV
                     EFYSVEDNFSIIKKLIDKGLFVDWFLFAADCMRLAPPLTLTRHEAEHACEIILAVLNE
                     "
     gene            4402..5391
                     /locus_tag="PLL28_12830"
     CDS             4402..5391
                     /locus_tag="PLL28_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HNF70256.1"
                     /translation="MKKLLFTILILGATAQFAAAPEPMSAPWGFWGHQRINKMALFTL
                     PEDLFGYYKPNIDYVTAHAVDPDKRRYAFADEAPRHYIDLDHYGKPPFENVPRKWNDA
                     VVKYSEDSLMDHGIVPWHIERMMYRLTEAFKQKDMNYILKTTADLGHYVADAHVPLHC
                     TENYNGQLTNQVGIHGFWESRIPELLGEDFDYYTGKAEFIPDVNAKVWEIVLESNSLV
                     DSVLSLEKILSTEFPADRKYAYETRGASTVRVYSKEYTERYNEMLDGMVERRMRESII
                     TLGSFWLTAWINAGKPNLTGIVMRPWTADELKEMEELDASYSKGTLKGREHEE"
BASE COUNT         1617 a         1098 c         1240 g         1534 t
ORIGIN      
        1 tttttttggt gagtggtgag tggtgagtgg gcagtgggtc agtgggtcag tggggcaatt
       61 tcacgactca cgactcacga ctcacgattc acgactcacg atattagtat caagaaccaa
      121 gtatctagta gtaagactaa tgcagtggaa tgtgccgtga attttctctg tgcacctctg
      181 tgttccgctc cgtgacctct gtggttaaat ggtgagtggg ggagtgaggc aatttcacga
      241 ttcacgattc acgattcacg agtcacgact caccactcac cactcacctt tacgtattct
      301 tcgtattttt gccccatgga cgcgaaaggt gaccaattcg atcatataaa agaaattcag
      361 gaagacatca acagcttccc tgttacagat gctgcttcgc tcgagcagtt cagaattcgt
      421 tttttgggaa gtaagaatat cctgaaggat gtattttccg ctattaaaga tgttcctaac
      481 gaaaggaaaa aagaattcgg acaattggtg aatgcactga agcaaagtgc agaacagcgt
      541 ttcgaaatcc tggaagcgca atacaaaaag caatctgaca agagtgccga tcaggtggat
      601 gtttcacgac cggcgataac aaacgctccc ggttcactac atcctgttac acttataaaa
      661 gatgaattaa tcggcatctt cacacgaatg ggctatacag tggttgatgg tccggaaatt
      721 gaagacgatt ggcataattt cagcgcactg ggtatgcccg aagatcatcc tgcccgcgat
      781 atgcaggata ctttttatat cagcacaaac cctgccgtat tattgcgcac ccatacttcc
      841 aatgtgcaga ttcgtgtaat ggaagaactc gccaaatcag gcaaacaacc accggtgcgc
      901 atcgttgctc tcggacgaac atatcgcaat gaaacaattt cggctcgcgc acattgtacg
      961 tttaatcaaa tagaattatt gtatatcgat gaaggcgtca cctttgccga tatgaaacaa
     1021 actttgttat atctggcgca agaattgtat ggagaaaata ctaccatccg cctgcgacca
     1081 agttatttcc cctttacgga accaagtgct gaaatggatg ttagttgctt cgtctgcggt
     1141 ggcgacggct gcgcgatctg taaacatagc ggctgggtgg aaattggcgg ttgcggtatg
     1201 gtggatccgg tggtactgga aaactgtggc atcgactcgg aaaaatatac cggcttcgct
     1261 tttggattcg gtctcgaacg catcgctatt ttgaaataca agatcaacga tattcgttta
     1321 ttcaccgaaa atgacctcag atttttgaag cagttcgaac agctttgatg aacgatatga
     1381 aaataattct cagcctattc gcttttattt gcctgctgca ggtagacatg catgcacaaa
     1441 acactacact tggtgccgat acctctaaaa tcactaagga tagtatcatc atgaccatcg
     1501 tgggtgagga tacccttatc ggtccgccca taacttttcg aaagtataat gaacgatttg
     1561 tcgacctgat gttttttgat ggtaactata atatcgtgag ctttcatatc gctgaaggca
     1621 gtacttcatt ccaactcatg ctcagtagcc ttgattcagc cacttattgt ttttcggttt
     1681 cccccgacga gtttaaaatg ctcatggcat acatgaagca atttaatgat gaattattgt
     1741 cgcagccgaa ttatcccgtg aagtgtaatt actgtcagac aattcaggta ttttggtacg
     1801 atggcgataa agaaaaaagt actttgaaaa gatcttatcg cataccacaa gtgttccccg
     1861 aaatattaga tttttgctac gatctgagtg atgataaacg cagatgtaaa ataagtttag
     1921 ataatttcat gtcgctggaa gggtttgagg ataacctccg aggacctgct agccaaaaat
     1981 aaaatcatgc catttagcta caccgactcg ctgaattatg tcagtaagct gactccgcgc
     2041 cgtgtgtgga atatgactaa aattttgggt tcatactatt atagtatgtg gactaaaaag
     2101 cctgttcaca agggcaatcc gatttctatt tcttttgagc ctactacttc ttgtaatctc
     2161 cgttgtccgg aatgcccaag tggattgcgc gcatttacaa gacctaccgg aatgctgaat
     2221 caggattttt tcagggatac gattgatgct atccataaag acctgacata cctcatattt
     2281 tattttcaag gtgagccgta tttgaatcct gcctttctcg acatggttgc atatgccaac
     2341 aagaaaaaga tttataccgc aacatctaca aacgcgcatt atctcaatga tgaaaatgcg
     2401 cgaaaaacta ttgaaagtgg tcttgacaga ttaatnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnatctc aatgatgaaa atgcgcgaaa aactattgaa agtggtcttg
     2581 acagattaat tatttctatt gacggcacta cgcaggatgt ttaccagcaa tatcgcgtag
     2641 gaggaaatct tgaaaaagtg ctggaaggtg cacgcaatat tgttaagtgg aaaaaagaaa
     2701 tgcgctcgaa aacaccattt gttatttttc aatttctcgt ggtaaaaccg aatgaacatc
     2761 aaattgaaga tgttaaaaaa ttgggaaaag aaattggtgt tgatgaagtg gccttcaaaa
     2821 ctgcacagat ctacgattat gaaaatggca acgatctgat tcctactatc gattattatt
     2881 cacgttatgc caagcaggat gatggtaaat atcgcatcaa aaataaaatg ctcgatcact
     2941 gctggaaaat gtggcatagc tgcgtaatca cctgggatgg cgccgttgtg ccttgctgct
     3001 tcgataaaga tgcagaacat aaactcggcg acctcaaaca acaaacattt tctgaattat
     3061 ggcgcggacc ggcctatcaa catttgagaa gctccttgtt gcgttcgaga agtgaaattg
     3121 atatttgtaa aaattgcacc gaaggcacca aggtgtgggc ttaattatta tatgcatcat
     3181 cgccaattat ttttgagaca tgtcgcccaa acaagcgatg ctccgcttgc catcgaaatt
     3241 acacaggcag cgggtaattg gttgtgggat gccgatggaa aaaaatatct cgaccttatt
     3301 tccggtatcg cagtgagcaa tattggtcat ggtcactcag cagttaaatc tgccatcaat
     3361 gatcaggttg ataaatatat gcacgtgatg gtgtatggtg aaatagtgca atcgccacaa
     3421 gtgcaatatg ctacatggtt gacgaatcat cttccttcaa cattaaattc tgtttatttc
     3481 gtcaactcag gttcagaagc tgttgaagga gcaattaaat tggccaaacg cgtgacagga
     3541 aaaacagaaa taattagttt tcgcaattcc tatcatggca gtaccatggg tgcattgagt
     3601 gttggaaata ccgaagaaag aaaaaatgcc ttccgcccgc taattcccga taatactgtc
     3661 cttacttaca acgatcctgc agccatacaa gcgatctctt cgcgaacagc agctgtgatc
     3721 attgaaccaa ttcaggcaga ggccggtgta ataaccccaa attccgaatt tttacaacaa
     3781 ttacgcgagg cttgttctac gcatgacgca atgtttattt tagatgaatg tcagaccggt
     3841 ttcggtcgca cgggtacttt gtttgcattc gaacaaatga acctcgttcc cgatatgtta
     3901 gtgcttggaa aagctgtagg tggaggtatg cctttgggat gttttattgc agatattgca
     3961 agcatgcaag cattcaccca tgatcccgtg ctcggacata tcactacttt cggaggtcac
     4021 cctgtatgtt gtgccgcggg attggctgca gcgcaggttt tggaagatga aaaattaatg
     4081 gagcatattc ccaatcttct gcaggttttt acatcgaaat tgcaacatcc taaaatcaag
     4141 tctttccgac acgccggcct tctgatggcc gttgaatttt attcagtgga agacaatttc
     4201 agcattatta aaaagctcat cgacaaggga ttattcgtcg actggttttt atttgccgcc
     4261 gattgcatgc gtctggcgcc accgctcact ctaaccaggc atgaagctga acacgcctgt
     4321 gagattatcc tagccgtttt aaatgagtga ccacattttt tcatctgacg gctattgatt
     4381 atccttgtat tgtaattcgt catgaaaaaa ttgctgttta ccatactcat cctcggcgca
     4441 actgctcagt ttgccgctgc tcccgaaccg atgtcggctc cctggggttt ttggggacat
     4501 caacgcatca ataaaatggc gctgtttaca ttgcctgagg atctttttgg ttattataaa
     4561 cccaacattg attatgtaac tgctcatgct gtcgatccag ataaaaggag atatgcattt
     4621 gccgatgaag cacctcgcca ttatatcgat ttagatcatt atggcaaacc accttttgaa
     4681 aatgtaccgc gaaaatggaa tgatgcggtt gtaaaatatt ctgaagactc attaatggat
     4741 catggtatcg ttccatggca tattgaacgc atgatgtatc gcttaaccga agcgtttaaa
     4801 caaaaggata tgaattatat cctcaaaaca actgccgatc tcggacatta tgttgcggat
     4861 gcacatgtgc ctttgcattg cacagagaat tataatggtc aacttacaaa tcaggttggg
     4921 atccacggtt tttgggaatc gcgtattcct gagttgctgg gagaagattt tgattattat
     4981 acaggcaagg cagaatttat tcctgatgta aatgcaaaag tttgggaaat tgtactggaa
     5041 agcaactccc tggtcgatag cgtcctgtcg cttgaaaaaa tattgagcac tgaatttccg
     5101 gccgatagaa aatatgcgta cgaaacaaga ggtgcttcaa cggtaagagt ttattcaaaa
     5161 gaatataccg aacgctacaa tgaaatgctc gacggcatgg tagagcgacg aatgcgagaa
     5221 agtataatca ctctaggcag tttctggcta acagcatgga ttaatgccgg caaacccaat
     5281 ctgacaggaa tcgtcatgcg cccgtggacg gcagatgaat taaaagaaat ggaggagctt
     5341 gatgcatcat actcaaaggg aaccttgaaa ggccgagagc atgaggaatg attggggagg
     5401 agcaaggaga gaggaggaag gagaaaggag aaatggtgtg tgtgggggta attagcaaat
     5461 ttgagaatta gcagattagc taattgggaa tgtgcttgca tgaagacgct cgtcttagtt
     5521 agcaagtgtg atagttagca aattagcaag tgtggagtgc ttttaacgct ggcgcgttaa
     5581 tagggaatg
//
LOCUS       DAOQHY010000198         5509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_8278_length_5509_cov_5.871470, whole genome shotgun sequence.
ACCESSION   DAOQHY010000198 DAOQHY010000000
VERSION     DAOQHY010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8278_length_5509_cov_5.871470"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="PLL28_12835"
     CDS             <1..1033
                     /locus_tag="PLL28_12835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70257.1"
                     /translation="TNTFAHTVMMQHWFLWHLKASFDPDYRNNFLTVATTQNFLSAGS
                     TIGLMTRSDKADQERLHEIATCSDPEIPVFYVRKAQYFGSLLQKPIIANDLKYLKSNI
                     SLGTLNNNTPPTLFIKKDGFESTDDTVYVVYDNVTDAYQTYIIEPGTLVNDYGGAAGY
                     EITLGSGTIEGDMLDADQLYSSAGSVAHFKFNEGYSNCAVSIDNENRFEIDALESLSP
                     QITCPGSVLAEFPGAVCISVPPISMGYVKIPFDVAPLRRGEMIDNYVMYPNPANEYFS
                     VYRKNTADTHDKILYIDVYSIFGHQLLHEESSEGERVQIGQLPSGTYIVILYLENGNM
                     EFEQLVKMK"
     gene            complement(1137..2771)
                     /locus_tag="PLL28_12840"
     CDS             complement(1137..2771)
                     /locus_tag="PLL28_12840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70258.1"
                     /translation="MKRISILATVIAISMSASAQSTTWEAVYNVIQTNCSGCHVAGHE
                     SGLDLSTDATTVYSSLVDIAPTNVTSAGKGYKRVFAGDPYRSFLFSKINNSLALDVNL
                     GAGEGTACPQGAAPLNNKDIELIRQWILYGAQESDTLVDIDLVNAFYDLDGEQSMPSP
                     PAPPAAGTGFQIHFGPFFLWPDTENEYFTKYPVNIPTDVEINRVDTYMGPYSHHFITY
                     KFVEPGVEASIEPGLHEGPDFFGVDLVTAHQYADSIILPPGTAFHWDYGTVLDLNSHY
                     INYSSDKPLACEAYINVYTQPVGTAIQTMYSVLLSNQSFNIPPTGDPYSDEATAFENG
                     HGEDELFIWGISTHTHKLGTDFDIYKRNDDHSRGEHIFDASCGATEGIPGCLDEIYDY
                     QHPPIRYWESMLPIKWKNGIIYEATWVNNGPDYIDFGPLSTDEMMVIFYFYVDDTTGL
                     NLPVAIPEITPQLTGIQLYPNPAHDNIFIQAETDLSKAMISICDLTGKTIQYSEPISM
                     GQDFYQVKLADVAKGIYLLQVITPDGAMFSQKIMKE"
     gene            complement(2839..3786)
                     /locus_tag="PLL28_12845"
     CDS             complement(2839..3786)
                     /locus_tag="PLL28_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNF70259.1"
                     /translation="MKIIIAGGTGAIGTAMSTFFLEKGNDVIVLSRQTLPSQRKLKII
                     KWDGEHIGEWRNECEGADVIINLCGKSVNCRYTEKNKLLILQSRLRSTRATGKLIGML
                     KRPPAVWFNAASATIYKGTLEGPQDEYTGETGSDFSMNVCTDWEREVMLAHTPFTRKI
                     YLRTSIVLDKNVGALPLYKRIASIGLGGAMGSGEQMMSWIHIDDVCRAVLFLIDKKEI
                     EGPVNITSPFPITNAVFMESLRRVLHMPFGVSNPEWMLRIGTSIIGTEMELLMKSRWV
                     YPQKLLAAGFHFNYPEINEALTDFFDPKAASSQVATSGR"
     gene            complement(3783..4580)
                     /locus_tag="PLL28_12850"
     CDS             complement(3783..4580)
                     /locus_tag="PLL28_12850"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNF70260.1"
                     /translation="MPTPLLTDPRLRKVYWKDLMYLTRSEIAWEVTLSLPWLIGSLIL
                     AARGWYAPALVCSFFFFLTGLRQSHNAQHRSVGISARATEWMLFALSVTMLASMHAVK
                     FNHLRHHKDPLGPSDIEAMSARMPWWMAILVGPFNILKQHGNALKLARPQLRRWVIIE
                     IVVILTGAATALWLIDIPWLSYHVIVMLTGECFTAFFAVWTVHHHCADDKVFSRTVRG
                     KWRTYIFYNMFYHTEHHLFPQVPTCHLHELAKRVDEHIPEISKKTVL"
     gene            complement(4634..5140)
                     /locus_tag="PLL28_12855"
     CDS             complement(4634..5140)
                     /locus_tag="PLL28_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNF70261.1"
                     /translation="MQLTEAKEKFVQSWGSLGSQWGINRTMAQIHALLLVSHEALSTE
                     DIMETLQISRGNANMNIRELINWQLVQKELRTGERKEFFVAEKDMWKVFRLILRERKK
                     REIDPMIQVIKSLQDIEGSSDQPDVREFREVMEELETLTHKGEKLMDLMIRSDEHWFI
                     NTFLKLIR"
     gene            complement(5251..>5509)
                     /locus_tag="PLL28_12860"
     CDS             complement(5251..>5509)
                     /locus_tag="PLL28_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70262.1"
                     /translation="YTDHDVMAQVEHFQNQFIRQDEASDELLHDLRAAEHVFSEISKT
                     NPAASHVLFDDHVELRDKATTHKTIFEDLRHEFQLFLAKYM"
BASE COUNT         1532 a         1174 c         1141 g         1662 t
ORIGIN      
        1 taccaataca tttgcacata ccgtcatgat gcaacattgg ttcttatggc acctgaaggc
       61 aagttttgac cctgattata gaaataattt tttaactgtt gccacgacac aaaatttctt
      121 aagtgcagga agcaccattg gtttaatgac cagatcagac aaagcagatc aggagcgatt
      181 acatgaaatt gcaacctgca gtgatcctga aatcccggta ttttatgttc gaaaggcgca
      241 atatttcggg tccttattgc agaaacctat cattgcaaat gatctcaaat atctgaaatc
      301 caatatttca ctaggtacac tgaataacaa cacccctccg accttattca ttaaaaaaga
      361 tggatttgaa tcaactgatg acacagttta tgtagtatat gataatgtaa cggatgctta
      421 tcagacatat attattgaac cgggaacctt ggtaaatgat tatggcggag cagcaggata
      481 tgaaataaca ttaggaagtg gaaccattga aggtgatatg ttagatgccg atcagctgta
      541 ttcttctgcc ggttctgttg cacattttaa attcaatgag ggatattcaa attgtgctgt
      601 atctattgac aatgaaaatc gatttgaaat tgatgcatta gaatcacttt cgccacagat
      661 aacttgtccg ggtagcgttc tagcagaatt tccgggtgct gtgtgcattt cagtgccgcc
      721 aatttcaatg ggatatgtga aaataccttt tgatgttgcg ccgctacgac gtggggagat
      781 gattgataat tacgtaatgt atcccaatcc tgccaatgaa tattttagtg tgtatcgaaa
      841 aaacactgct gatactcacg ataaaatatt gtacatagat gtatattcga tttttgggca
      901 tcaactattg catgaagaat cctccgaagg agagcgcgtg caaataggac aattgccctc
      961 aggaacttat attgttatct tgtatttgga aaatggaaat atggaatttg agcaactggt
     1021 gaaaatgaaa tagatgccaa ggtgtttaat attagggtgc ggaatgcata tgtttcgcac
     1081 cctaatacta tacatatttg tatgtattac ttgagccttt ttgcttataa aaattcttac
     1141 tccttcataa ttttctgcga aaacatggcg ccatcgggtg tgatgacttg gagcaaataa
     1201 atgcctttcg ctacatcagc taattttact tgataaaaat cttgtcccat ggaaatcggt
     1261 tcactatatt gtatggtttt accggtcaga tcacaaatgc tgatcatagc cttcgacaga
     1321 tcagtttctg cctggataaa aatattatca tgtgccgggt ttggatataa ttgaataccg
     1381 gtcaattgtg gtgttatttc cggtatagca accggtaaat tcaaacctgt tgtgtcatcc
     1441 acataaaaat aaaatatcac catcatttca tcagtactca atgggccgaa gtcgatataa
     1501 tccggtccgt tgtttaccca tgttgcttca taaatgatac catttttcca tttaataggc
     1561 agcatgcttt cccaatatct gatgggtgga tgctggtaat cataaatttc atcaagacaa
     1621 cccggaatgc cttctgtagc accacaagat gcatcgaaaa tatgttcacc gcgcgaatga
     1681 tcatcgttgc gtttataaat gtcgaaatcg gtacccagtt tatgtgtatg tgtgcttatt
     1741 ccccagataa acaattcatc ttcaccgtgt ccgttttcaa aagcagtagc ttcatcagaa
     1801 tatggatcac cggttggcgg aatattgaaa ctttgattac tcaacaaaac agaatacatg
     1861 gtttgtattg ccgtacctac cggctgcgtg taaacattta tatatgcttc acatgcaagg
     1921 ggcttgtctg atgaataatt aatataatgt gaattcaaat ctaataccgt accgtaatcc
     1981 cagtgaaatg ctgttcccgg aggcaaaata atgctatcgg catactgatg tgccgtaacc
     2041 aaatcaacac caaaaaaatc aggtccttca tgcaaacccg gttctataga agcttcaact
     2101 cccggttcaa caaatttgta ggtgatgaag tgatgtgaat atgggcccat atatgtatcc
     2161 actctgttta tctctacatc ggtaggaata tttaccggat attttgtgaa atattcattt
     2221 tctgtatcag gccataaaaa gaatggaccg aagtgaattt gaaatcctgt acctgctgcc
     2281 ggaggtgcgg gtggcgatgg catactctgt tcaccatcta aatcgtaaaa agcattaaca
     2341 agatcgatgt ctaccagtgt atcactttcc tgcgcaccgt ataaaatcca ttgacgaatt
     2401 aattcaatgt ctttgttatt taatggcgcc gctccttgcg gacaagcagt tccttcgcct
     2461 gctcccagat ttacatcaag ggcaagactg ttattgattt tgctgaataa aaaactgcga
     2521 tatgggtcgc ctgcaaatac gcgcttgtaa ccctttccag ccgacgttac atttgtagga
     2581 gcaatgtcta cgaggctgct ataaacagtg gtcgcgtccg tacttagatc caatccgctt
     2641 tcatgacctg ccacgtgaca tccgctgcag tttgtttgaa tgacgttgta aacagcttcc
     2701 caggtggtag attgggcaga tgccgacatg ctgatggcga taacagttgc gaggatactt
     2761 attcttttca taattgcctt tgaggctacg aagttagcga attctgcaat tgaaaatcgt
     2821 aaattccttg tcaaatcctc atctgcccga cgtggctacc tgcgatgaag ctgctttggg
     2881 gtcaaaaaaa tctgttaagg cctcattaat ttccgggtaa ttaaaatgaa aaccggcagc
     2941 caacaatttt tgcggataca cccatctgct tttcatcaga agttccattt cggttccaat
     3001 aatcgatgtg ccgatgcgca acatccattc cggattcgaa acaccaaatg gcatatgcaa
     3061 aacacgtcgc aatgattcca taaatacagc attcgttatt ggaaatggcg aagtgatatt
     3121 taccggtcct tctatttctt ttttgtcgat gagaaataat acggcgcgac aaacatcatc
     3181 aatatgaatc caactcatca tttgttctcc actacccatt gctccgccta atccaattga
     3241 agcaatgcgt ttgtataatg gcaatgcacc aacattttta tctaaaacta ttgaagtgcg
     3301 cagataaatt tttcgtgtaa atggtgtgtg tgccaacatg acttcacgtt cccagtcggt
     3361 gcatacattc atactgaaat cgctacctgt ttctccggta tattcgtctt gcggaccttc
     3421 caatgttcct ttgtaaatgg ttgccgaagc ggcgttgaac catacggcag gagggcgttt
     3481 cagcatgcct atcaattttc ctgtcgctcg tgtactgcgc aggcgcgatt gcaaaatgag
     3541 taacttattt ttctctgtgt atctacaatt tacactcttc ccgcagagat taataatcac
     3601 atctgcgcct tcacattcat tgcgccattc gccgatatgc tcgccatccc atttgattat
     3661 ttttaatttg cgttgtgatg gcaatgtttg tcgcgataat acaattacgt cgtttccttt
     3721 ttcaagaaaa aatgttgaca tggcggttcc gattgcaccg gttccgccgg cgatgataat
     3781 tttcatagta cagttttttt actgatttca ggaatatgtt cgtctacacg ttttgctaat
     3841 tcatgcaaat gacatgtagg cacttgtgga aataaatgat gttctgtgtg atagaacata
     3901 ttgtagaaaa tgtaggtgcg ccatttacca cgcactgtgc gactaaaaac tttgtcatcg
     3961 gcacagtgat gatgcacagt ccacactgca aaaaacgcag taaaacattc acccgtcagc
     4021 atcacaatca cgtggtagct cagccatgga atatcgatca gccacaaggc agttgcagca
     4081 ccggtcagga tcactacgat ttcaattatg acccatctcc gcaattgtgg tcttgccagt
     4141 tttagggcat tgccatgttg tttgaggatg ttgaatggac caacaagaat tgccatccac
     4201 caaggcatac gggcactcat cgcctcgata tcgctcggtc cgaggggatc tttgtgatgt
     4261 ctcaggtgat tgaatttcac cgcgtgcatc gacgccagca tggttacact cagggcaaat
     4321 aacatccatt cggtagccct ggccgaaatg cctacggagc gatgttgggc attgtgcgac
     4381 tggcgcaatc ccgtaaggaa aaagaaaaac gagcaaacca aagccggtgc gtaccatccc
     4441 ctagcagcta atatcaacga gccaatcagc cagggtagcg atagtgtcac ttcccaggct
     4501 atttcactgc gtgtgaggta catcaggtct ttccaataaa cttttctaag tcgggggtcg
     4561 gtaagtagtg gggttggcat gacatttatt tttatatttt cagtaatatt tgaaagttta
     4621 tagtcaaaaa aaatcagcgt atcagtttta ggaaagtatt gataaaccaa tgctcgtcgc
     4681 tgcggatcat caggtccatg agcttttcgc ctttgtgtgt gagtgtttcc aattcttcca
     4741 tcacctccct gaattcacgc acatcaggct ggtcgcttga accttctatg tcttggagcg
     4801 atttgatcac ttgaatcatg gggtctatct cgcgtttttt gcgttcacgg agaatgagtc
     4861 ggaatacctt ccacatatcc ttttcagcca caaaaaactc ttttcgctcc cctgttctta
     4921 attccttttg caccaattgc cagttgatga gctcgcggat attcatgttg gcattacccc
     4981 tgctgatttg cagggtttcc atgatatctt cggtggacaa tgcttcatga ctcacaagca
     5041 gcaaggcgtg aatctgagcc atagtgcggt ttatacccca ttggctgccc agacttcccc
     5101 agctttgaac aaatttttcc ttggcttcgg tgagttgcat aagccaaagg tataacaact
     5161 tttcaaactt tcaaacattt gtgaaagttt tttttaaaat agaaaagccc ggctgctgac
     5221 ttccgggcct aaggttggat aatcggacct ttacatgtat ttggccagga acagttggaa
     5281 ttcgtggcgg aggtcttcaa aaatggtttt gtgggtagta gccttatcgc gcagctccac
     5341 gtgatcgtcg aacagcacat gtgatgcggc gggatttgtt ttcgaaattt cactgaacac
     5401 gtgttctgca gccctcaggt cgtgcaacaa ttcatcgctg gcctcgtcct gacgtataaa
     5461 ctggttttga aagtgttcta cctgtgccat cacatcatga tcggtataa
//
LOCUS       DAOQHY010000199         5442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_8449_length_5442_cov_5.144607, whole genome shotgun sequence.
ACCESSION   DAOQHY010000199 DAOQHY010000000
VERSION     DAOQHY010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8449_length_5442_cov_5.144607"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..535
                     /locus_tag="PLL28_12865"
     CDS             197..535
                     /locus_tag="PLL28_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70263.1"
                     /translation="MRNVLLTLLLSGIMTAAFADNQNEICSSDPMRETIANMISHETP
                     FTEFDLEGIVSIQFMVDDQHVIHVKGISGGNTFLIDHVLTSLQNKVLNCDCVNAGTLY
                     TLKLQYVQYS"
     gene            600..1649
                     /gene="mltG"
                     /locus_tag="PLL28_12870"
     CDS             600..1649
                     /gene="mltG"
                     /locus_tag="PLL28_12870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNF70264.1"
                     /translation="MTGTRKKWLIGIGIVLLVGGVIVGIIGYSLYNKIMAPNVKADKE
                     ASVFVYHGDNLDSIIARCARQNLLRSPENFRWWAQRQGLDNLLKPGRYAVSNGMSTRE
                     LVNLLASGRQTPVKVTFNNLRTKYDLAGRIAQQLEPDSSDIIAFFDTTTMFSSVGLNK
                     ENFMTIFIPNTYELYWTSTPSEFVARMIKEHDVFWNADRLNAAKEIGLSPEQVYILAS
                     IVYSENEKAADEAPRIAGVYMNRLQKGMKLEADPTVKFAIGDFTIKRILFADLEIDSP
                     YNTYKYPGLPPGPIHMPPIAYIDAVLHYEHHDYIFMCASGDGSGYHLFAKTLAEHNRN
                     RDIYIRTLNKAGIKR"
     gene            complement(1709..2587)
                     /locus_tag="PLL28_12875"
     CDS             complement(1709..2587)
                     /locus_tag="PLL28_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HNF70265.1"
                     /translation="MQKLLIVLLLLVGLNATAKAQDDLLSMLGDDSTTTEYVKNAFKS
                     SRVINGQSMEMIPKGALDFRILHRFGEVSGGAYEFFGLDQASMRMGFDYGILKNLSVG
                     VGRSTAQKELDGFVKYRILQQSTGAKVMPFSLVYVGGVTCNGMKWADTSVTNYFTSRL
                     AFYNQLVIGRKFNESITLQLSPTFLHRNLVTYDADPSDIYSLGVGGRVKVTRRIALTG
                     DYFYVFNNNDSTAVNPLSIGVDIETGGHVFQLHFSNCVGMNERAFLLDTKGQWSKAEV
                     RFGFNLSRIFQLADSH"
     gene            complement(2652..3215)
                     /locus_tag="PLL28_12880"
     CDS             complement(2652..3215)
                     /locus_tag="PLL28_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNF70266.1"
                     /translation="MMMRKVVMVFAVQLMFLSFVQAQGKIYTKTGTINFDATADMEKI
                     AAVNKKVTAVIDTKSGAMEFAVLMKSFEFEKALMEEHFNENYVESDTYPKATFKGTIS
                     NLSDIKFTTDGTYTANVTGNLTMHGVTKPVTTTGTFTVKNGKVTAKSTFKVLLSDYNI
                     TIPSLVAANISKEVTITVDLALEPLPQ"
     gene            complement(3232..3666)
                     /locus_tag="PLL28_12885"
     CDS             complement(3232..3666)
                     /locus_tag="PLL28_12885"
                     /inference="COORDINATES: protein motif:HMM:NF024275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70267.1"
                     /translation="MKKNWKIILLVVVILGGIGFWYGMQQYNRKPKGAGDMKAEFTMT
                     AADLTKAFTDDEAGSGTKYNAKPMLISGTVRGVEKDDAGIYTITLAGDGDMSNVVCTM
                     SNEESGLDTVKEGDALNIQGFCNGAQTMLGTDVLMNRCAIKK"
     gene            complement(4155..4667)
                     /locus_tag="PLL28_12890"
     CDS             complement(4155..4667)
                     /locus_tag="PLL28_12890"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNF70268.1"
                     /translation="MREFIIALSLPVSGIISFPQSGCGQTICTPKEIRTEIIINAPSA
                     EAWKLLTDFDAYSGWHPYILKIEGKIALKEKIKVTTIDADSTADQFSAFVLELEPEKK
                     LSWGGSLGFIFSAKHYFIIEPINDDQIRFVQGEYWHGLFGKHYGEKIYLDTFQKFIAM
                     NEKMKILLEG"
     gene            5003..>5442
                     /locus_tag="PLL28_12895"
     CDS             5003..>5442
                     /locus_tag="PLL28_12895"
                     /inference="COORDINATES: protein motif:HMM:NF036423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HNF70269.1"
                     /translation="MATIKVVLRKKIRKDGTFPLALSIVKDRKPTYIHIGKNILETDW
                     DAIRQKVKKSHLNATFLNNYIRMRLADATDRLLEMELRKEEFSAVNLKNQSDASTNLK
                     FLNQSALYLSNLRDSGKFNRLSAEAPRINRFKEFLGGKDIPFQD"
BASE COUNT         1570 a         1110 c         1105 g         1657 t
ORIGIN      
        1 atcaagtatc aagtatcagg tatcaagtat caggtatcaa gtatcaggta aaaaatgcac
       61 gattcacgat tcacgattca cgactcacca ctcaccactc acccactcac ccactttaac
      121 ttttccttaa gtaacctttc tgtcatttct acgccttaag gaagtattta attattcatt
      181 catcaaatat catactatgc gtaacgtatt attaaccctg ctactaagtg gtataatgac
      241 tgctgcattt gctgataatc aaaacgaaat atgttcttca gatccaatgc gtgaaactat
      301 tgccaatatg atttcgcatg aaacaccttt caccgaattc gatctagaag gaatagtgag
      361 cattcaattt atggtagatg atcagcatgt tatccatgtc aaaggtattt ccggtggcaa
      421 tacatttttg atagatcatg ttttaacctc ccttcaaaac aaagtattga attgcgactg
      481 tgtgaacgcc ggaaccttat acacgttaaa actgcagtat gtgcagtatt cttaactaac
      541 ttttttcatg ttgtagatca ggagcctgca tgtgcgggct ctttcttatt tttacatcta
      601 tgaccggcac taggaaaaaa tggctcatcg gtataggcat tgtgctgctt gtgggaggcg
      661 ttatagttgg tattatcggc tactcgttat acaacaaaat catggcgcca aatgtcaaag
      721 ccgataaaga agcttctgtt ttcgtttatc atggcgacaa cctcgattcc ataatcgcaa
      781 gatgcgccag gcaaaaccta ttgcgttcgc cggaaaattt cagatggtgg gctcaacgtc
      841 agggattaga taacttgctc aaaccggggc gttacgctgt ttcaaacggc atgtcgacac
      901 gcgaattagt caatttactg gcaagcgggc gacaaacacc tgtgaaagtt accttcaata
      961 atctccgaac aaaatatgat ctggcaggaa gaatcgcgca acaattggaa cctgattcat
     1021 ctgacatcat agcatttttc gatactacca ccatgttttc atccgtagga ctgaataaag
     1081 aaaatttcat gacaatcttt attcctaata cctatgaatt gtactggaca tcaacgcctt
     1141 cggaatttgt tgccagaatg atcaaggagc acgatgtatt ctggaatgca gatcgcttga
     1201 atgcagctaa ggaaatcgga ctcagtcccg aacaagtata cattttggca tctattgtat
     1261 attccgaaaa tgaaaaagct gccgatgaag ctccgaggat tgccggcgtt tacatgaaca
     1321 gattacagaa aggtatgaaa ctggaagccg atcctacggt aaaattcgcc atcggtgatt
     1381 ttaccattaa aagaattctg tttgctgatt tggagatcga ttcgccatac aatacttata
     1441 aatatccggg tcttcctccg ggtcctattc acatgcctcc aatcgcctac attgatgccg
     1501 tgttgcacta cgaacatcac gattatattt tcatgtgtgc cagtggcgat ggttcaggtt
     1561 atcacctgtt tgctaaaacg ctggcagaac acaatcgcaa tagagatatt tatatccgca
     1621 ccctgaacaa ggctggaatt aaaagataag gcattccaaa aaaaaatccc gaccttgttg
     1681 atcgggattt tttttgaata ttattttatt aatggctgtc ggccagttgg aaaatacgag
     1741 ataaattaaa tccgaagcgt acttcagctt tcgaccattg tccctttgta tccagaagaa
     1801 atgcgcgttc attcatacct acacaattgc tgaaatgcaa ctggaatacg tgaccaccgg
     1861 tttcaatatc aacaccaatg ctcaaaggat ttacggcagt gctatcgtta ttattgaaca
     1921 cataaaaata atcgccggtg agtgcaatac gacgtgtaac cttcacgcga ccgccaacgc
     1981 ctaatgaata aatatcactt gggtctgcat cataggttac gaggttgcga tgcaaaaatg
     2041 ttggtgaaag ctggagggta atgctctcat tgaattttct tccaatcaca agttggttgt
     2101 aaaatgcaag tcgcgaagta aaataattgg tcacagatgt atctgcccat ttcattccgt
     2161 tgcaggtcac gccgccaacg tacaccaatg aaaagggcat caccttagcg ccggtagatt
     2221 gttgtaggat acgatatttt acaaatccat cgagctcttt ttgagctgta gatctgccga
     2281 cacctactga tagatttttt aggatgccat agtcaaaacc catacgcatg ctcgcttgat
     2341 caaggccaaa gaattcataa gctccaccgc tcacttcgcc aaaacggtga aggatacgga
     2401 aatcaagtgc ccctttcggg atcatttcca tcgactgtcc attgattacg cgtgaagatt
     2461 taaaggcgtt ttttacatac tcagttgtag ttgagtcatc gcccagcatg ctcaataagt
     2521 catcctgagc ttttgcagtt gcgttcagtc ctacgagaag gagcagaaca attaataatt
     2581 tttgcattgg ttggtatttg aggaaacaaa aaaaattcct gaacaaagat atgttcagga
     2641 atttaaaagt tttactgtgg gagtggttcc agtgcgaggt cgacggtaat ggttacctct
     2701 ttactgatat ttgctgccac cagcgaagga attgtgatat tatagtcgga tagcaagacc
     2761 ttgaatgtcg attttgctgt caccttgccg tttttcacgg tgaaggtgcc tgtagtggta
     2821 acaggctttg taacaccatg catggtaagg tttcctgtaa catttgcggt ataagtgcca
     2881 tctgtagtga atttgatatc acttaaattc gagatggttc ctttgaatgt tgcttttgga
     2941 taagtatcac tttcaacata attctcgttg aagtgctctt ccatcagtgc tttttcaaat
     3001 tcaaacgatt tcatcaacac tgcaaattcc attgcaccgc ttttggtgtc gataacagcc
     3061 gtaacctttt tgttaactgc agcgatcttt tccatgtcgg cagtggcatc aaaatttatg
     3121 gtgcctgttt tcgtgtagat cttgccttgc gcctgaacaa aactcaggaa catcaattgc
     3181 acagcgaaaa ccattactac ctttctcatc atcatattca ttgttttatt gttatttttt
     3241 aatagcacat ctattcatta atacatcagt tccaagcatg gtttgagcac cattgcagaa
     3301 accctggata tttaaagcat caccttcttt aacggtatct agaccacttt cttcattcga
     3361 catggtgcat acaacattgc tcatgtcgcc atcgccggca agggtgatgg tataaattcc
     3421 tgcatcatct ttttcaacac ccctaacggt tccgcttatg agcatgggct tagcattata
     3481 ttttgttcct gagcccgctt catcatcagt aaatgccttt gtaagatcgg cagcagtcat
     3541 ggtgaattct gccttcatat cgcctgcgcc cttaggtttg cggttgtatt gctgcatgcc
     3601 gtaccaaaag ccaatcccgc caaggattac aacaacaagc agtataattt tccagttctt
     3661 tttcatagtt gggtttttat gtgttaaatg ttattacaag tatcgtgcct attgatgcca
     3721 ggtaccttgc attgattggc gggtcaaagt tacaaccctc tgtgcaagcc atccttagac
     3781 acttttagct gatttccgtg ttttttaagg gatttgggtt ccttttatgc cttgtaaacg
     3841 taaaaatgag cattttggtt ccttctgaga cggaggtaaa ttgttgattt agaaggggtt
     3901 ggggggtgag tgggtgagtt tacctcgccg aacaggcggg tggttgagtt tacctcgccg
     3961 aacaggcggg taggtgagtt taacctccga gcagattggt cgtgagaaag gggcgatagt
     4021 tgcattgcgc ctttgcatct ttgcgtcttc gcgcgaaaaa agggttactg attgtttggt
     4081 gatcctgaag gataatagtt cttcatcact ttgcctgtcg aagctatctt ctattgaatc
     4141 gttttgaatt acaattatcc ttccaaaagt attttcattt tttcattcat ggcgataaac
     4201 ttttgaaatg tatccaggta tattttttcg ccataatgtt tcccgaataa cccatgccaa
     4261 tattcacctt gcacaaaacg tatttgatcg tcatttatag gttcaattat aaaataatgt
     4321 ttggcactaa aaataaaacc caatgatcca ccccaggata gttttttttc cggctctaac
     4381 tctaaaacaa atgccgagaa ttgatctgca gtagaatccg catcgatggt ggttacttta
     4441 attttttcct tcaaagcgat tttgccctca atttttaaaa tataaggatg ccagcctgaa
     4501 taggcatcaa aatcggtaag taatttccat gcttcagctg aaggagcgtt gatgattatt
     4561 tctgtccgga tttctttggg agtgcatatt gtttgtccgc aaccggattg tggaaatgat
     4621 ataatgcctg atacaggtaa actaagtgca ataataaatt cgcgcataat attttgaagt
     4681 caggagtttt gctgatttca aatgtagcta aatattgggg agcttaaggg gcgtgacttg
     4741 aacctatcat acggcaggca ggcccgcaaa aaggttacat tgaagataat tctacgcgag
     4801 aattaattta agtacccggg gcgggacttg aacccgcacg gacattactg cccacaggat
     4861 tttaagtcct gcgtattttg atctgatgtg tatcaaaatg taatgaaagt cttgagaata
     4921 tgctggttat ggtcaattta ggtggatata agttcataat aattcaaatt atgttatacc
     4981 tttgctatac ccgaatcatg ttatggcgac aattaaagta gttttaagga agaaaatacg
     5041 aaaagatggc acctttcctt tggccttgag cattgtaaag gataggaagc ccacctatat
     5101 tcacattggt aaaaatattc tagaaaccga ttgggacgca ataaggcaaa aagtgaaaaa
     5161 gtctcaccta aatgcaacat ttcttaacaa ttacataagg atgaggttag cagacgcaac
     5221 cgataggttg ctggaaatgg agcttagaaa ggaagaattt agtgccgtta acctcaaaaa
     5281 ccagtctgac gcctctacca atttaaaatt tctcaaccaa tctgcccttt acttaagcaa
     5341 cctaagagac tcgggcaagt ttaaccggtt gtctgccgaa gcacctcgaa ttaaccgctt
     5401 taaggaattt cttggaggta aagacatacc ctttcaggat at
//
LOCUS       DAOQHY010000200         5397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_8561_length_5397_cov_4.533882, whole genome shotgun sequence.
ACCESSION   DAOQHY010000200 DAOQHY010000000
VERSION     DAOQHY010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8561_length_5397_cov_4.533882"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1741
                     /locus_tag="PLL28_12900"
     CDS             <1..1741
                     /locus_tag="PLL28_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF70270.1"
                     /translation="FLTGTLFVGLANLFQVYAPHFIGDMIDAVAENADTVINGTDSEK
                     ASAVYRFILINALLFIGITLLRGVVMFFMRQTIIVMSRKIEYDQKNELFRKYEFLTPA
                     FFKRNNTGDLMSRVAEDVGRVRQYVGPAIMYFLNLLFNFIFGIWMMLSINPTLTLYVL
                     LPLPILSVSIYFVNSIINKKSEQIQAQLSHLTSLAQESFSGIRVIQAYAREKQTVNFF
                     ADACDDYKTDSLSLARIDAMFQPLMVLLIGMSILLTIFVGGWQVIQGNLSPGNLAEFL
                     FYVNMMTWPVTSLGWVVSIIQRAAASQKRISEFLDLQPDIVSEGLAPKQVKGRITFKN
                     VHFTYPDSGVKALDGVSFDIGIGETVAIVGRTGSGKSTIADLIFRLYDANDGSILIDG
                     EPITSFNLTDLRNGVSMVPQDVFLFSDSIAHNIAFAAPDADVERVKQFAQFASVDKEI
                     ESFKSGYDTIVGERGVTLSGGQKQRVSIARAFMKESPVIILDDCLSAVDAGTEHIILG
                     NMRRYLEKRTAIVITHRITSLLEFDKIIVMDQHQVAEYGTHDELMALNGLYAKMVNAQ
                     RDVTTSELKNNVR"
     gene            1819..2259
                     /locus_tag="PLL28_12905"
     CDS             1819..2259
                     /locus_tag="PLL28_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HNF70271.1"
                     /translation="MEHENSNRNDSVFSRKIRAGRRRTYFFDVRTTKGNDYYITITES
                     KKRFDGNGYDRHKLFIYKEDFNKFADELQQVIDHIKGLLPDFDFDAFAHRYDENGEHY
                     DGGNHQHHDDDREQYNDNSNSSSSDNTSTDNNSGHHYGDEDLKL"
     gene            2378..3628
                     /locus_tag="PLL28_12910"
     CDS             2378..3628
                     /locus_tag="PLL28_12910"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70272.1"
                     /translation="MSKWCSRSMQFLVVILTFLSLDSKGQNLVPNNGFETNDGTPTGY
                     GQWYHATGWSDVNNYPTFLWPYASPDYLHNTGAGGADLPVCTFGTVSPFAGNAVMGFV
                     TWLQSTPDFREYLSIPLSSPMIVGTAYNVSFWITNGSASWYNGYSSNRIGARFSIGTL
                     TQATHEPIGGTPQCELVTDLWTTTWQNVSFVYTPDQAYNQITIGNFYNDASTSHSYHV
                     ASLSAGAYYFIDEVVVEPAVPLPVELLAFEAVQTDHGADISWNTASEFDVSSYVVSRS
                     SDGVNFNPLGEISATGNATTSADYLLLDDEPLFGNNYYQLEVVNLNGEVDETYLTALY
                     VDGTASRPVVYPNPSRGSVTISIPIGLPLAGIQIFDISGRLIREINTLSATVPIGIND
                     LTTGTYVIKFISTIDIDNQLLIFE"
     gene            complement(3647..5242)
                     /locus_tag="PLL28_12915"
     CDS             complement(3647..5242)
                     /locus_tag="PLL28_12915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70273.1"
                     /translation="MKQLGNISAYLLIITILIPICEVRGQNTNISDGYLFDGEPFITI
                     NPSNGSHMVVAWMGVNGLDLVQIMTRVSTDGGNSWSAEYAQPHIAGGYTSADPSMDWD
                     SDGNLYLCYIDYSPFGTSGKTIVRKSADGGFTWGDPVEVIDAFDDPGEVPVDRPWLVI
                     DRSGGLDDGFQYVTTKPAPWIPFPNRNYLTVSKNGGLSFEDWRYIDTTGWQIGDFIQA
                     PMAAPAVGPDGTLHCVYPAWEPTENLLPRFIHASSGNGGDGFSYHEMLESFGGDLNAD
                     TSAKTGYQLLVDPTDPHHLVFLFVFRPLGDNDIYCLESANSGVNWSTPLRINADPAGN
                     KVMQDLVWGDFDGEGNLVVTWRDRRNAVDTGYAVASEIWGAVRYKDSIAFSNDFRISS
                     ALAAFDSVLYGNGNDFMGVQLFNDTLYAVWGDTRNDYLNIWFSKQAVGSSTPTGVINL
                     GSEQLFEITAYPNPAGNYLMVEGDNISRLQIRSASGQVYLDQSITTTGDIRLSLENIP
                     AGNYFLAAFIGNDVAVRQIAITK"
BASE COUNT         1508 a         1256 c         1156 g         1477 t
ORIGIN      
        1 gttcctcacc ggaactttgt ttgtaggtct cgcaaacctg tttcaggtat atgcaccgca
       61 ctttataggc gatatgattg atgccgtagc cgaaaatgct gataccgtaa tcaacggcac
      121 cgatagcgaa aaagcttccg ccgtttaccg cttcattctc atcaatgcac tgcttttcat
      181 cggaattacc ctccttcgtg gtgtggtcat gttcttcatg agacaaacca ttatcgtgat
      241 gagccggaaa attgaatacg accagaaaaa tgaactgttc cgcaaatatg aattcttaac
      301 cccggccttc ttcaaacgca ataataccgg cgatctgatg agccgtgtgg ctgaagatgt
      361 cggaagggta aggcaatatg taggaccggc catcatgtat ttccttaacc tgttgttcaa
      421 cttcattttc ggaatctgga tgatgctttc cattaacccg acactcaccc tgtacgtgct
      481 gctgccattg ccaatattgt cggtttccat ctattttgta aatagcatta tcaacaaaaa
      541 aagcgaacaa attcaagccc aattatcgca ccttaccagc cttgctcagg agtcattcag
      601 cggtattcgt gtaatacaag cctatgcacg tgaaaagcaa actgttaact tttttgcgga
      661 tgcatgcgat gattataaaa ccgactcgct tagccttgcc cgcatcgacg ctatgttcca
      721 gccattaatg gtgctgttga ttggaatgag catactgctt accatttttg tagggggatg
      781 gcaagtgata caaggcaacc tgagtcccgg caacctggcg gaatttctct tttatgtgaa
      841 tatgatgacc tggcctgtta cctcgttggg atgggtggtt tccatcatcc aacgcgcagc
      901 agcatctcag aaaaggataa gcgaattcct cgacctgcaa ccggatatcg tatccgaagg
      961 gcttgctcca aaacaagtaa agggtcggat aaccttcaaa aacgtgcatt ttacctatcc
     1021 tgattcaggt gttaaagcct tggatggagt gagtttcgat ataggaattg gcgaaactgt
     1081 agctattgtt ggcagaacag gctcagggaa atcaactatt gcagacctga ttttcaggct
     1141 gtatgatgcc aacgacggca gtatattaat tgatggagaa cccatcacat ctttcaacct
     1201 gaccgatctg cgtaatggag tcagtatggt gcctcaggac gttttcttgt tttctgactc
     1261 aatagctcac aatatcgcat tcgccgcacc tgacgcagat gtggaacgtg ttaaacaatt
     1321 cgctcagttt gcctcagtcg ataaagaaat tgaatcgttt aagtcgggat acgataccat
     1381 agtaggggag cgaggtgtta ccctcagcgg aggtcaaaaa caacgtgtgt ccattgcgcg
     1441 cgccttcatg aaagagtctc cggttattat tctggatgat tgcctgagcg ccgttgatgc
     1501 aggaacagaa catatcatcc tgggaaatat gcgtcgctat ctggaaaaac gaaccgctat
     1561 cgtaattact catcgtatca cttcattgct tgagtttgat aaaatcattg tcatggacca
     1621 acatcaggtg gcggaatatg gcacacatga tgagcttatg gcactaaatg gactctacgc
     1681 aaaaatggta aacgcacagc gcgatgtcac cacttcagaa ttaaaaaata atgtccgtta
     1741 gtttgttttt caaaatcttt ttatatttcg gtcaaaatac aaaggtaaat acctacctac
     1801 taccctaaac tgactattgt ggagcacgaa aacagcaatc gcaacgacag cgtattctca
     1861 aggaaaattc gcgctggaag gcgcaggact tacttctttg acgtaagaac aacaaaagga
     1921 aacgattact acatcaccat tacggaaagc aagaagcgct tcgacggaaa tggatacgat
     1981 agacacaagc tgtttattta taaggaagac ttcaacaaat ttgcggatga actacaacag
     2041 gtaattgatc atatcaaagg tctacttcct gatttcgatt tcgacgcatt cgctcatcga
     2101 tatgatgaaa atggcgaaca ttatgatggc gggaatcacc aacatcacga cgatgatcgc
     2161 gaacaatata acgataacag taattcatca tcctcggaca atacgtcaac ggataataat
     2221 tccggccatc attatggcga cgaggatctg aaactctaac aacgacacac aaccggaaag
     2281 ccgctcctca tgggcggttt ttttgtgtct gttggtacga aaaaaggaga tttttgtcct
     2341 gtttttgcac ctgacagcat atgtaacttc ggtgcatatg agcaagtggt gtagccggtc
     2401 catgcaattt ctagttgtaa ttctcacttt tctatcgctt gatagcaaag gccaaaacct
     2461 cgttcccaac aacggtttcg agacaaacga cggtacaccg accggttacg ggcaatggta
     2521 tcacgctacc ggttggtctg atgtaaataa ctatcccacc ttcctttggc catacgcaag
     2581 tcccgattat ttgcacaata ccggagcagg cggagcagat ttgccggttt gcacttttgg
     2641 aaccgtttct cccttcgcag gcaatgctgt aatgggtttc gtcacctggc ttcaatctac
     2701 acccgatttc agagaatatc tttctatacc attgtccagc cccatgattg tcggaactgc
     2761 ctataatgtg tcgttttgga taaccaacgg ctccgcaagc tggtataatg gttattcatc
     2821 caatagaatt ggtgccagat ttagtattgg tacgctaacc caggcaacac acgaaccaat
     2881 cggaggaaca cctcaatgcg agctggttac cgacctgtgg acgaccacct ggcaaaacgt
     2941 aagtttcgtt tacacccctg atcaagccta taatcaaatt actattggca acttctataa
     3001 cgatgcatca acatcacatt cataccatgt agccagtctt agcgcaggag cctactattt
     3061 tattgatgaa gtggttgtag aacccgctgt tccgcttcct gtcgagttat tagcctttga
     3121 ggcagttcaa accgaccacg gcgctgatat cagctggaat acagcctcgg aatttgatgt
     3181 ttcatcgtat gtggtttcaa ggagtagcga tggcgtcaat tttaaccctc taggtgagat
     3241 atcggccacc ggtaacgcaa caacctccgc cgattacctg ctgttggacg atgaacctct
     3301 ttttggaaac aattattacc aactcgaagt ggtcaacctg aatggagaag tagatgagac
     3361 ttatctaaca gccttgtatg ttgatggaac cgccagccgg cctgtcgtct atccaaaccc
     3421 atcaagaggt tctgttacta tctcaatacc gataggatta cctcttgccg gcattcaaat
     3481 attcgacatt tccggacgat taattcgcga gattaatact ctaagcgcca cagttcctat
     3541 aggtatcaat gacctgacta caggaacata tgttatcaaa tttatatcga ctattgatat
     3601 agataatcaa ttattaatat ttgaataaca attacttata aaaattttat ttagtaattg
     3661 cgatttgtct tactgcaacg tcattgccaa taaaggccgc caggaagtaa tttccggcag
     3721 gaatattctc gagtgataaa cgaatatctc ccgttgtggt gatggattga tcaaggtaga
     3781 cttgaccgga ggctgaacgt atttgtaatc gtgaaatgtt gtcaccttca accatcagat
     3841 aattgccggc aggattgggg taggctgtaa tctcaaacaa ctgctcactg ccaaggttga
     3901 tgacaccggt aggtgttgat gagccaactg cttgcttact gaaccaaatg ttgagatagt
     3961 catttctggt atctccccaa acggcataca gtgtgtcgtt gaacaattgc acacccataa
     4021 aatcgttgcc attaccgtat aatacactat caaaggcagc caatgcactg ctaatacgga
     4081 aatcgttgct aaacgctatt gaatccttat agcgcacagc cccccatatc tctgatgcga
     4141 cagcatatcc ggtgtcaacg gcgttccgtc tgtcgcgcca ggtaaccaca agatttcctt
     4201 ctccatcaaa atcgccccaa accaggtcct gcatcacctt attacctgcc ggatctgcat
     4261 tgatacgtaa gggtgtactc cagttaactc cgctatttgc cgattcaaga caataaatgt
     4321 cattatctcc caagggtctg aaaacgaaca gaaacaccag gtgatgtgga tcagtaggat
     4381 caaccaatag ctgatatccg gttttggcac tggtatcggc attcaagtcg ccaccaaagg
     4441 attccagcat ctcgtgataa ctgaaaccat cgccgccatt acctgaagat gcgtgaataa
     4501 accttgggag caagttttct gtcggttccc aggcagggta gacgcagtgc aaggttccgt
     4561 cgggacctac agccggagca gccatcgggg cctgaatgaa atcgccgatc tgccagcctg
     4621 ttgtgtcaat atatcgccaa tcttcgaatg acagcccacc attcttacta acggtgaggt
     4681 agtttctgtt tgggaagggt atccatggag cgggttttgt tgtgacatac tgaaatccgt
     4741 catctaagcc gccactcctg tctattacca accaaggacg atctactggc acctcacccg
     4801 gatcgtcaaa ggcatcaatt acctccaccg gatctcccca tgtaaaaccg ccatctgccg
     4861 atttgcggac tatcgttttg ccggaagtcc cgaatgggct gtaatcgata taacacagat
     4921 acaggtttcc gtcactatcc caatccatag atggatccgc cgaggtgtat ccccctgcaa
     4981 tatgtggttg cgcatattca gccgaccagc tattgcctcc atccgtgctt acccgagtca
     5041 tgatctgaac caggtccaat ccgtttaccc ccatccaggc aacaaccata tgtgaaccat
     5101 tacttgggtt aatcgttata aatggttcac catcaaatag atatccatct gaaatattcg
     5161 tattttgacc tcggacctca caaattggta taagaattgt gatgatcaat agataggccg
     5221 atatatttcc tagctgcttc atttgtggtt gggtgaattt gttttgtctg cagttaaagg
     5281 taatacctat atagccaatt tgtgattctt tcctgattca gatggtagtg agtagtggta
     5341 ttaggtatca ggtatcaggt atcaagtatc aggtatcaag tatcaagtat caggtat
//
LOCUS       DAOQHY010000201         5387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_8577_length_5387_cov_5.638597, whole genome shotgun sequence.
ACCESSION   DAOQHY010000201 DAOQHY010000000
VERSION     DAOQHY010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8577_length_5387_cov_5.638597"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="PLL28_12920"
     CDS             complement(<1..1221)
                     /locus_tag="PLL28_12920"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF70274.1"
                     /translation="MNTTAYISIIADMSDRITTLRTANEAQAYFSEQFRKMFPITHCA
                     MYRVSERDDTFYRTYDFAAPKANVQPKAGDKITLSKAISDDKDTFVILHHQEPWFVVP
                     RQQVKQKGEVLLLIRNAEKVVAIIHGIAPEPIPENGFQNDTARVFSNFFIQVEGGLQL
                     RTQINEHQIELSGHIANNNDTLFQLNERLIQYNEELQQFAYSASHDLQEPLRNIASYI
                     NLFIKQYGSTINEEGLEYLQFASDGAQRMQQLIRDLLTYNKLDQQQEPMALFEGNQML
                     QEALYNLRMSVEESNALILYPELPVLYGNPSQIILLFQNLIDNAIKFRKNDEPLIFIE
                     MEEKPEHWEFKVVDNGMGIAAQYQQKIFGVFYRMHGPDQIRGSGLGLSICKKIVERHH
                     GTIKVDSKPGKGTTF"
     gene            1360..1974
                     /locus_tag="PLL28_12925"
     CDS             1360..1974
                     /locus_tag="PLL28_12925"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNF70275.1"
                     /translation="MEAPETYITILQSATGSYSESGSKFLGYSFAIVDESEIKILRDQ
                     LSKEHHKAVHVAFAARLGFEGENERSSDDGEPSGSAGKPILNELKSRALTQTCVLIVR
                     YFGGKKLGIPGLIHAYRAAAIDCLNNSGTKEVVLYDDYEISCHMDHMPVVLHELNKSG
                     ARVIDSEFAENCNFKIEIPRTQHAAFTKTLQNLWQATATYIGMH"
     gene            2021..>2526
                     /locus_tag="PLL28_12930"
     CDS             2021..>2526
                     /locus_tag="PLL28_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70276.1"
                     /translation="MHAFSLSNSISINTDIDKACKNAFPTLIATDIRLANTEHSPKAD
                     YYYFNQYYYNIPIAGAGVKWIVPHNGDTSWLFGNIYDTINMSFLEAEISVRFDLIHEQ
                     LNRLGVTEHIDESKCWYISGNTLSLHRTYSYRFPDGVTYTFHFNRAGELIATEDNRSY
                     FTLPDTTVA"
     assembly_gap    2527..2626
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2627..3867
                     /locus_tag="PLL28_12935"
     CDS             <2627..3867
                     /locus_tag="PLL28_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70277.1"
                     /translation="IATEDNRSYFTLPDTTVAGKVFLPDPLTTASVEYGDDYVDEDDA
                     DLTVLNDQRFDVRVPAQLIDGIFYLRTDSLLLKDINIPVIPIASATTDTMVFTRSESG
                     FEDVNTMYHITNMYQYLASIGYEALHNFYLEVDPHGASGADQSFYTGLPFPSIQYGEG
                     GVDDAEDADVIIHELTHAFSDHASPNTNTGLERRAVDEGYGDYFAVSYSRTYSDFLWK
                     NVFCWDGHNEFWFGRNTDTDKHYPEDNNDNYYASSEIWSGALMDIFDLIGKEATDRIV
                     CEALYGSFPNMIMPDAAQILLNAEMMLYGGIYHDIMFDALYQRGLVWPVDVVQQNFDN
                     IRITNTLGYASGNEPIFITLETPGNGSWQLFNLDGKLIAEKSFYGSSCAIDVHPTDGQ
                     LLLLKVNAQGKTVSTIIANF"
     gene            complement(3874..4725)
                     /locus_tag="PLL28_12940"
     CDS             complement(3874..4725)
                     /locus_tag="PLL28_12940"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNF70278.1"
                     /translation="MANPEKRILVPTDFSQPSYAALQYAIYIANRTDSRISLIHFADR
                     AELKEKGGSIAGYKEVLRALDQQLQDMKNASGAAFRMHAEVISTTQSIADEISDYGLK
                     IDAVMACLGTAGKSARKSSHSLGDNTGKLVKSVDFPVLTCRNVKEPIRFRNFLLPIDL
                     TKYTTEKVERIIRFAQGFEATIHLVAVSEFLEEYFTSREALGEKMEEAAETIRKSGLK
                     CTTEMIRHDMVNNSIKIYASEIDADLLVIMSHGENKFNQLIFGSRINKVISHSDIPVL
                     SFRPEDD"
     gene            4772..>5387
                     /locus_tag="PLL28_12945"
     CDS             4772..>5387
                     /locus_tag="PLL28_12945"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HNF70279.1"
                     /translation="MHTAPQASELIRNSDGSIYHLHLFPEQVADIVITVGDPDRVAQV
                     SKHFDAIEFQQQKREFITHTGSVGNKRITVISTGIGPDNIDIVLNELHALKQFDFQGH
                     TAKTIEQPFTIIRFGTSGSIQADIPVGTLLMSELGIGIDNVMHFYNHRNNDAETALLE
                     QFTLHCYFHHTIHPYIASADTSLLTHFAKEFISGITLTAPGFYAP"
BASE COUNT         1552 a         1114 c         1037 g         1584 t
ORIGIN      
        1 aaacgtggtt ccttttccag gcttggaatc aactttaata gtgccatgat ggcgttcgac
       61 aatctttttg caaatagaca agcctaatcc tgatcctctg atctgatcgg ggccatgcat
      121 gcgatagaat acgccaaata tcttttgctg atactgagcc gcaattccca tgccattatc
      181 tacaacttta aattcccaat gttcaggctt ttcttccatt tcaataaaga tgaggggctc
      241 atcgtttttc ctgaacttga tagcgttgtc tattagattt tgaaacaata aaataatttg
      301 tgaaggatta ccatataaca caggcaactc aggatataat atcaatgcat tactttcttc
      361 gaccgacatc cgcaaattat acaatgcctc ttgcaacatt tgattccctt caaacaaggc
      421 cataggctcc tgttgctgat cgagtttatt ataagtaagg agatcgcgta tcaattgctg
      481 catccgttgt gcaccatcag atgcaaattg cagatattcc agaccttctt catttattgt
      541 gctgccatat tgcttgataa ataaattaat atacgatgca atattccgca gcggctcctg
      601 aaggtcgtga gatgctgaat atgcaaattg ttgcaactct tcattgtatt gaattaaccg
      661 ctcgtttaat tgaaataatg tgtcgttatt attggcaata tgtcctgaaa gttcgatctg
      721 gtgctcattt atttgagtgc gcaattgcaa accaccttct acctggataa aaaaattaga
      781 aaagacgcgt gccgtatcat tttgaaatcc attttccgga atcggctcag gagctatgcc
      841 atgaataatg gccacaactt tttcagcatt ccgaattaat agcagcacct cacctttttg
      901 ttttacttgc tgtcgaggca caacaaacca tggctcctga tgatgcaaga tcacaaaagt
      961 gtctttatca tcgcttattg cttttgataa agtaatcttg tctcccgcct tgggttgcac
     1021 atttgccttt ggagctgcaa aatcatacgt gcgataaaaa gtatcatccc gctcactcac
     1081 tctatacata gcacaatgtg tgatgggaaa cattttacgg aactgttccg aaaaatatgc
     1141 ctgagcctca ttggcagtcc gcagcgtagt aatgcggtcc gacatgtcgg caataatgga
     1201 aatgtaagct gtcgtgttca aaatgtcgtg tgtagtacaa ttttaccctt acagcctgaa
     1261 atcaccaaac tgttagacaa gttttaacca aacgaaggcc tttttttatg tttttctaaa
     1321 taaaaaaacg aagaaaacac gaaagcttaa ctttgccctg tggaagcgcc agaaacttat
     1381 atcaccatcc tgcaaagcgc tacaggaagc tattctgagt cgggaagcaa gtttttgggg
     1441 tatagttttg ctatagtcga tgaatcagaa ataaaaatcc ttcgcgacca gctttcaaag
     1501 gagcaccaca aagccgttca cgtagctttt gccgccagat taggttttga aggcgaaaac
     1561 gaacgaagca gcgatgatgg cgaaccatcc ggcagtgccg gcaaacccat tcttaacgaa
     1621 ttgaaaagca gagcgttaac tcaaacctgc gtcttaattg ttcgatattt tggcggtaaa
     1681 aaactcggaa ttcccggact tattcatgcc tatagagccg ctgcaataga ttgtttaaat
     1741 aattccggaa caaaggaggt ggtattgtat gatgattacg aaatctcgtg tcacatggat
     1801 catatgcctg ttgttttgca cgaattaaat aaatcgggag ctcgtgttat tgattcagag
     1861 tttgcagaaa actgtaattt taagattgaa ataccgcgca ctcagcatgc tgcatttaca
     1921 aaaacattgc aaaacctttg gcaggctact gctacctata ttggtatgca ttaatctgtt
     1981 taatagcagg aatgccttcg cgcagttgaa tatcaatcgc ttgcatgcct tttccttgag
     2041 taattcaatt tctattaaca ctgatattga caaggcgtgt aaaaatgctt tcccaacttt
     2101 aatagcaaca gacatcagat tggcaaatac tgaacatagt cctaaagctg attactatta
     2161 tttcaaccaa tattattata atattcccat tgcaggagca ggggtgaaat ggattgtacc
     2221 acataacggt gatacatcat ggctgtttgg taatatttac gatactatta acatgtcatt
     2281 tcttgaagcc gaaattagtg tgcggtttga tcttattcat gaacagctaa acagactcgg
     2341 tgttactgaa catatcgatg aatcaaaatg ctggtatata tccggaaaca cactcagttt
     2401 gcatcgcacc tattcctatc gttttcctga tggcgtaacc tatacctttc attttaacag
     2461 agccggagaa ttaatcgcta ctgaggacaa tcgcagctat ttcactttac ccgacacaac
     2521 agtagcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntaat cgctactgag
     2641 gacaatcgca gctatttcac tttacccgac acaacagtag caggaaaagt atttctccct
     2701 gatccactta caactgcgag tgtggaatat ggagatgatt atgtagatga agacgatgcg
     2761 gatttaaccg tattaaatga ccaacgcttt gatgtgcgtg tgcctgctca attgattgac
     2821 ggcatatttt atttgcgcac cgattcgttg ttgttaaagg atataaatat tccggtaatt
     2881 ccaattgctt ccgcaaccac agatacgatg gttttcactc gcagcgaaag tggatttgaa
     2941 gatgtgaata ccatgtatca tatcacgaat atgtaccaat atttggcttc tattggatat
     3001 gaagcattac ataattttta tcttgaagta gatccccacg gcgcttccgg tgcagatcaa
     3061 tcattttata ccggattgcc atttccaagt attcaatatg gcgaaggtgg tgtagatgat
     3121 gccgaagatg ccgatgtaat catacatgag cttacccatg ctttcagcga tcatgcttca
     3181 ccaaatacca ataccggact tgaaagacgc gctgttgatg agggatatgg cgattatttt
     3241 gcagtaagct attctcgcac atacagcgat tttttatgga agaatgtttt ttgttgggat
     3301 ggtcacaatg aattttggtt tggcagaaat acagataccg ataaacacta tcctgaagat
     3361 aacaatgata attattatgc cagcagcgaa atttggtcgg gcgcactgat ggatattttc
     3421 gacttaattg gaaaagaagc taccgacagg attgtttgcg aggcacttta tggcagcttc
     3481 ccgaatatga ttatgccgga tgccgcacaa atattattga atgcagaaat gatgctttat
     3541 ggcggaattt atcatgatat tatgtttgat gccttatatc agcgcggact agtgtggcct
     3601 gtagacgttg ttcaacaaaa ttttgacaac attcgcatta ccaatacctt agggtatgca
     3661 tccggaaacg agccgatatt cattacactt gaaactcccg gaaatggttc atggcagcta
     3721 tttaaccttg acgggaaatt aattgcagaa aaatcatttt atggcagctc ttgtgccata
     3781 gatgttcatc ctaccgatgg acaattatta ttactgaaag taaacgctca ggggaaaact
     3841 gtttcgacaa taattgcaaa tttttaaccc ggcttaatca tcttcaggcc taaaactcaa
     3901 caccggaata tccgagtgcg aaatcacttt gttaatgcga gaaccaaaaa tgagttgatt
     3961 gaatttattt tctccatgac tcatgatcac cagcagatcg gcatcaattt ctgatgcgta
     4021 tatcttgatg gaattgttca ccatatcatg acggatcatt tcagtagtgc attttaagcc
     4081 gcttttgcgg atggtttctg ccgcttcttc cattttttcg ccaagggctt cgcgcgaagt
     4141 gaaatattct tctaaaaatt ccgaaactgc aacgaggtgt atcgttgcct caaatccttg
     4201 agcaaaacga atgatgcgtt caaccttttc tgtggtgtac ttcgtaagat ctatcggcaa
     4261 cagaaaattc ctgaaccgaa tgggttcctt aacatttcgg catgtgagca ccgggaaatc
     4321 gacacttttt accagttttc cggtattatc gccaagacta tgtgacgatt tacgcgcact
     4381 tttgcccgca gttcccaggc atgccataac tgcatctatt ttgagcccat aatcgctgat
     4441 ctcatcagct atgctttgtg tagtactgat tacctccgca tgcatgcgaa aagcagcgcc
     4501 gctggcattc ttcatatcct gaagttgctg atccaaagcc cttaacactt ccttataacc
     4561 ggcaatagag ccgcctttct ccttcaattc tgccctatcg gcaaagtgaa tgagagaaat
     4621 acgtgaatca gtgcgattgg cgatatagat agcgtattgt aacgctgcgt atgaaggttg
     4681 agaaaaatca gtggggacaa ggatacgttt ttcgggattt gccatagccg ctgcaaaatt
     4741 aatccaaaat tttacattta attttacggt catgcacacc gctccgcaag cctccgaact
     4801 catcagaaat agtgacggat ctatctatca cttgcatttg ttccccgaac aggttgcaga
     4861 catcgtaatt accgttggtg atcccgacag ggttgctcag gtcagcaagc actttgatgc
     4921 cattgaattt caacaacaga aaagagaatt catcacacat accggctctg tcggcaataa
     4981 aagaattact gtcattagta caggtatcgg tcctgataat attgatatcg tattaaatga
     5041 attacatgca ttaaaacaat tcgatttcca aggtcatact gctaaaacaa ttgaacaacc
     5101 atttaccatt atccggtttg gcacttcagg ttccattcaa gccgatatac ctgttggcac
     5161 attattgatg tcggaattgg gaattggtat agataatgtc atgcactttt acaatcaccg
     5221 gaacaatgat gctgaaacag ctttgcttga acaatttaca ttacactgtt attttcatca
     5281 cacgatacat ccatacattg caagcgccga cacctcccta ttgacgcatt tcgcaaaaga
     5341 attcatttcc ggcatcacac tcaccgcacc gggattttat gctcctc
//
LOCUS       DAOQHY010000202         5300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_8813_length_5300_cov_7.362440, whole genome shotgun sequence.
ACCESSION   DAOQHY010000202 DAOQHY010000000
VERSION     DAOQHY010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8813_length_5300_cov_7.362440"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="PLL28_12950"
     CDS             complement(<1..753)
                     /locus_tag="PLL28_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70280.1"
                     /translation="MKLHSIRLSLLICLITFGINYMQACSMSKITFNGVTYVGNNEDS
                     WHTDPRIWFEPGAGSKRGACYVGYADKWPQGGMNIDGLVYDAFTMYDGNVQPNGKPIA
                     ENLPIFLRDIMQSCGIVDEVYKYAISFDRHNFDRAMMLFVDTTGKYLVMEADTMIIGN
                     DSTFLLANFSFSKTKDLSTVSLGRYQRGRTFLEKHEIGGLDFCRALMDTMSECRERKG
                     DGTLYTSLYDTKRRLISLCFYHNYDRVHILSLN"
     gene            complement(760..1614)
                     /locus_tag="PLL28_12955"
     CDS             complement(760..1614)
                     /locus_tag="PLL28_12955"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNF70281.1"
                     /translation="MKLSVKLFLHIFLFCSSFLMAAQDSTFSVITFNLRYDNPADAEN
                     NWHYRKQEIADFIQLRSPEIFGIQEGLIHQLAFLDSALTRYQYVGKGRDDGAQKGEFS
                     AIFFDTSKVTLIYDSTFWLSLTPSEPSVGWDAALPRICTFARLVTKNSGDTLNVWNMH
                     LDHMGITARYKSIALLVVMINDFDKALWLQPMIIMGDLNSTPLDDPAHTMLKFAEDTR
                     TATLTPPLGPDATFNGFAPLNTESKRIDYIFVRNLTTIAQEHIDTKRSNGLWLSDHLP
                     VMATLSFK"
     gene            complement(1639..2289)
                     /locus_tag="PLL28_12960"
     CDS             complement(1639..2289)
                     /locus_tag="PLL28_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70282.1"
                     /translation="MNSSFGYIYVFIIVCSIGCTRTSNETKSKTLVNSNISEDVDSSS
                     GSDSLDVIEFIKVFALDRADTFIVGDVNGDRKVDKAIIEPATFIYRDGKVDSQYVNIS
                     FTTDIPVIRHYNGFHGLIANVGDLDGNKTDELIYYPDWFIGNMASIYIYGLRDGEWKL
                     FADGSQRRDIIFETKDPIKYLQSRVEKIDNSSFKLTEITMNENAEFIDSTSIVFIH"
     gene            complement(2392..2967)
                     /locus_tag="PLL28_12965"
     CDS             complement(2392..2967)
                     /locus_tag="PLL28_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 domain-containing protein"
                     /protein_id="HNF70283.1"
                     /translation="MTNDYISSVKNQFEYYKMLGEKTFDQVTDEQLFWKFNEESNSIA
                     TIVKHLSGNMLSRWTDFFTSDGEKEWRKRDEEFENDIQSREDMLKVWNEGWNCLYHVI
                     DHLTADDLEKIIYIRNQGHSVVEAINRQLAHYPYHVGQIVFIGKMVMDEKWKSLSIPK
                     GNSKAFNKEKFDKPKERRHFTDEYLGKNDGK"
     gene            complement(3038..4162)
                     /gene="mqnC"
                     /locus_tag="PLL28_12970"
     CDS             complement(3038..4162)
                     /gene="mqnC"
                     /locus_tag="PLL28_12970"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HNF70284.1"
                     /translation="MQLDTLYSKALAEKWLTVEEGIYLFEHAPTAALMQIADTLRKRK
                     KPGNKVTWIIDRNLNTTNICVANCKFCNFYRVPGHAEGYITDMETYKVKIEETIRYGG
                     EQLLLQGGHHPELGLDFYTGIFRGIKEMFPNVKLHALGPPEIHHIATLGGYSYTYVLS
                     ELMKAGLDSLPGAGAEILIDRVRKIISTGKCLSDEWLDVMRAAHQLGLTTSATMMFGH
                     IETIEERFLHLEKIRQVQSEKPEHAKGFIAFIPWPFQDEGTTLRKIRRAKNETTPDEY
                     IRTIAMSRIFLPNIENIQASWLTVGKKTAQICLHAGANDFGSIMIEENVVSAAGAPHR
                     FTYKSIQEAIREAGFEPQLRTQQYEDREIPANIQEQVINY"
     gene            complement(4282..>5300)
                     /locus_tag="PLL28_12975"
     CDS             complement(4282..>5300)
                     /locus_tag="PLL28_12975"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HNF70285.1"
                     /translation="GATEVCNGIDDNCDGNIDEGLTFETWYADADGDMYGDAGASVST
                     CDGAPAGYVADAADCNDADAAVNPGATEVCNGIDDNCDGNIDEGIDLSIAISPDGVIT
                     LCKPDAITLEATAGFTSYQWYKNGSPLAGETGATYTTNKPAYYQVEGFIGTCTSGLSA
                     VQAVAVVESPNANISTPDGTNLCIASPLLIKASYDAAYTYVWYKDGIEIAGATTAEID
                     ATETGDYYCVITNASGCTRTTATVTIINECRSGVADAAGISLYPNPASSSLVVSLTGI
                     DAENAIIAMVDVMGNTISTSSVAINSGNGTATIDVSAVAAGVYFITVQGNDVILNQQI
                     VVTK"
BASE COUNT         1567 a         1157 c          971 g         1605 t
ORIGIN      
        1 attcaatgaa agaatatgta cgcgatcata gttgtgataa aagcacaatg aaattaatcg
       61 acgtttggta tcatacaatg aagtatacag ggttccatca ccttttcttt ccctacattc
      121 cgacatcgta tccatcaatg ctctgcaaaa atcaagtcct cctatttcat gtttttccag
      181 aaatgtgcga cctctttgat atctgcccaa cgatactgta gaaaggtctt tagttttaga
      241 aaaagaaaaa ttcgccaaca aaaatgtgct atcgttacca ataatcatgg tatctgcttc
      301 catcaccaga tattttccgg tggtatcgac aaacaacatc attgctcggt cgaaattatg
      361 tctgtcgaag ctgatagcgt acttatatac ttcatcaacg attccgcagg actgcattat
      421 atcgcgcagg aaaatcggca ggttttctgc aattggtttt ccattgggtt gaacatttcc
      481 atcgtacata gtaaatgcat catacaccaa accgtcgatg ttcataccac cctgaggcca
      541 tttatcggcg taaccgacat aacaggcacc gcgtttgctt ccggctcccg gttcaaacca
      601 gatgcgtgga tcggtatgcc agctgtcttc attattacct acataagtaa ctccattaaa
      661 tgtgatctta ctcatgctgc aagcttgcat gtagttaatt ccgaaggtga tcagacaaat
      721 aagcaacgag aggcgaatag aatgtagctt catgcttaac tatttgaaag aaagtgtagc
      781 cataacaggt aaatggtccg acaaccacaa tccattcgat cgttttgtgt cgatatgttc
      841 ttgagcaatg gttgtaagat ttcgcacaaa aatatagtct attcgcttac tttcagtatt
      901 aagcggtgca aatccattaa atgtagcatc aggaccaagt ggaggggtga gcgtcgctgt
      961 tcttgtatcc tcggcaaatt tgagcatggt atgtgcagga tcatcgagtg gtgtgctatt
     1021 taaatcgccc ataatgatca taggttgaag ccacaatgcc ttatcaaaat catttatcat
     1081 taccacgagc aaggctattg atttataccg tgcggtaatg cccatgtgat cgaggtgcat
     1141 attccataca ttcaaggtat cgccggaatt tttagtcacc aaacgcgcaa aggtgcaaat
     1201 acgcggaagc gctgcatccc atccaacaga aggttcacta ggtgtaagtg aaagccaaaa
     1261 ggttgaatca taaattaatg taaccttaga tgtgtcgaaa aaaatggcgc taaactcacc
     1321 tttttgtgca ccgtcatcac gaccttttcc gacatattga tatcgcgtaa gtgcactatc
     1381 cagaaaagct aactgatgga taagcccttc ctgtatgcca aaaatctcag gtgaacgaag
     1441 ttgaataaaa tcggctattt cctgttttct ataatgccaa ttattttccg catcggcggg
     1501 attatcgtat cgaaggttaa atgttattac actaaatgtg gagtcttgtg ctgccattaa
     1561 gaatgaagag cagaataaaa aaatatggag aaacagtttt actgatagtt tcatggtatt
     1621 aatattcgta aaataaattt aatgaataaa cactattgaa gttgaatcaa taaactcagc
     1681 attttcattc atagtaattt cagtcaactt aaaagaacta ttgtcaattt tttctaccct
     1741 actctgcaaa tacttgatag gatccttggt ttcaaagata atatctcgtc tctgggatcc
     1801 gtcggcaaac aacttccatt caccatctcg caatccatat atgtaaatac tcgccatatt
     1861 accgataaac caatcaggat aataaatcaa ctcatccgtc ttgttaccat caaggtcgcc
     1921 gacatttgca atcaatccat gaaaaccatt ataatgtctg ataacaggaa tatcggttgt
     1981 aaaggatatg ttcacatact gactatccac cttaccatcg cgataaataa aggtagccgg
     2041 ttcaatgatg gccttatcaa cttttctgtc gccatttaca tcgccaacaa taaatgtatc
     2101 agcgcgatct aatgcaaaga ctttaataaa ttctatgaca tcaagcgaat cactaccgga
     2161 agacgaatct acatcttcag aaatattgct attaactaat gtcttacttt ttgtttcatt
     2221 ggaagtcctt gtgcacccga ttgagcaaac tattataaat acataaatat atccaaagga
     2281 tgaattcatt tcaacttaca atctcacttc aattgacact ccaagtttaa gttatttaga
     2341 ttcttgttaa tgattatctt tcagacaccc attaagtatg taaaccaata atcacttccc
     2401 atcattttta cccaaatact catccgtaaa atgacgcctt tcttttggtt tatcaaactt
     2461 ttctttgttg aaggcttttg aatttccttt gggaatcgac aacgatttcc atttttcatc
     2521 cattaccatc ttacctataa aaacaatttg tcctacgtga tagggataat gtgccagttg
     2581 cctgttaata gcttctacaa ctgaatgacc ttgatttctg atgtagataa ttttctccag
     2641 atcatcagca gttaaatgat caattacatg atacaaacag ttccaaccct cattccatac
     2701 tttcaacatg tcctctctgc tttgaatatc gttctcaaat tcctcatcac gctttctcca
     2761 ttctttttct ccatccgagg tgaagaaatc tgtccaacga gacaacatgt tgccgctcaa
     2821 atgtttcacg atagtagcga tcgaattact ttcttcgtta aacttccaaa agagctgttc
     2881 atccgttacc tgatcaaagg ttttttcgcc aagcatttta tagtactcga attgattttt
     2941 aacgcttgaa atataatcgt tggtcataaa caaatagatt tagatggtaa tatatatata
     3001 ttactacaaa atacacacaa cgcgataagc acaattatta atagttgatc acctgttcct
     3061 ggatattggc aggaatctcg cgatcttcat attgctgtgt gcgcaattgc ggttcaaatc
     3121 cggcttcacg aatcgcttct tgaatagatt tataagtgaa tcgatgcgga gcgcctgctg
     3181 cactgacaac attttcttct atcataatac tgccgaaatc attcgctccg gcatgcaaac
     3241 aaatttgcgc tgtttttttg ccgacagtaa gccagcttgc ctgaatattt tcaatattgg
     3301 gaagaaatat tctgctcatg gcaatggtgc ggatatattc gtcgggagta gtttcatttt
     3361 tcgcgcgacg gatttttcgc agtgtagtac cttcatcctg aaacggccaa ggaataaaag
     3421 caataaatcc ttttgcatgt tcaggttttt cactttgtac ctgacgaatt ttttcgagat
     3481 gcaaaaatcg ttcttcaata gtctcgatat gaccaaacat catcgtagca ctggtagtta
     3541 aaccaagctg atgggctgcg cgcattacat ccaaccattc atcgctcaaa cattttcctg
     3601 tgctgataat cttgcgtacg cgatcaatta atatttcagc acctgcaccg ggcagactat
     3661 caagacctgc cttcattaat tcagacaaca cataagtgta cgagtaacca cctagtgttg
     3721 cgatgtgatg gatttcagga ggaccgagtg cgtgtaattt cacattcggg aacatttctt
     3781 ttatgcctct gaaaataccg gtataaaaat cgagtcccaa ttccggatgg tgaccaccct
     3841 gtaacaagag ttgctctccg ccgtagcgaa tggtttcttc aatcttcacc ttatatgttt
     3901 ccatatcggt gatgtagccc tctgcatgtc cgggaacgcg atagaaatta caaaacttac
     3961 aattcgcaac gcagatattg gtagtattca aattgcgatc gataatccat gttactttat
     4021 tgcccggttt tttgcgtttt ctgagtgtat cggcaatttg catcaatgcc gccgttggtg
     4081 cgtgttcgaa aaggtaaatc ccctcctcta ccgtgagcca tttttcggca agtgctttgc
     4141 tatataaggt atccagttgc attcaaaagt tcaacaatca aacggatgaa tcgttcgcca
     4201 aagatagggt tgtacatgaa ataaaagaag ccaccccata aagggcagct tctccaaatt
     4261 caatacacac aataatttta attattttgt aaccacgatc tgctgattga gtatcacatc
     4321 atttccttga acagtaatga agtaaacacc tgcagctacg gcagatacat ctatggtagc
     4381 ggttccatta ccactgttga tggcaacact agaagtgctg atggtattac ccattacatc
     4441 aaccatggca atgatggcat tttcagcatc aatacctgtc aaactaacca caaggcttga
     4501 agaggcaggg tttggataaa gactaatacc ggcagcatcg gcaacacctg acctacattc
     4561 attgatgatg gtaactgttg ccgtagtgcg tgtacaacct gatgcattgg taataacgca
     4621 ataataatcg cctgtttctg ttgcatcgat ttcggcagtg gttgcacctg caatttcaat
     4681 tccatcttta taccatacat aggtatatgc tgcatcgtaa cttgctttga tcagcagcgg
     4741 actggcgata caaaggttgg taccatcagg agtagaaata tttgcattcg ggctttcaac
     4801 aacggctact gcctgaactg ctgaaagacc gctggtgcag gttccaatga atccttctac
     4861 ctgataatat gcaggcttgt tggttgtgta ggttgcacct gtttcacctg caagcggact
     4921 gccattttta taccactgat aagatgtaaa tcctgcagtt gcttcgagtg tgatagcatc
     4981 aggtttacac aaagtgatta cgccgtcggg agaaatggca atagacagat cgataccttc
     5041 gtcgatattg ccatcgcaat tatcatctat gccgttacat acttctgttg ctcccggatt
     5101 tactgcagca tcagcatcat tacaatcagc agcatcggcc acataaccgg caggagcgcc
     5161 gtcacaagtg cttacagagg cacctgcatc gccatacata tcgccgtcag catcagcgta
     5221 ccaagtttcg aaagtcagac cttcatcgat attgccatcg caattatcat ctatgccgtt
     5281 acatacttct gttgctcccg
//
LOCUS       DAOQHY010000203         5274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_8888_length_5274_cov_5.071853, whole genome shotgun sequence.
ACCESSION   DAOQHY010000203 DAOQHY010000000
VERSION     DAOQHY010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8888_length_5274_cov_5.071853"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PLL28_12980"
     CDS             <1..241
                     /locus_tag="PLL28_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNF70286.1"
                     /translation="EVSSAKVITDKFTGKSRGFGFVEMPNDGQKAMDELNGAEIMGKT
                     IAVSEARPQTERKPFNNNRGGGGGNYGGGGGGRRW"
     gene            complement(317..2053)
                     /locus_tag="PLL28_12985"
     CDS             complement(317..2053)
                     /locus_tag="PLL28_12985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70287.1"
                     /translation="MKPICIVFFTAIMLIQQLKAQTPYAVLNPGQTDYNSKFGVYPDH
                     EGNFYLVNKSYYPQDLDAGAGVLTYGPPNQQVLIAKYTSSFELLWSAHIEQATILESK
                     AAFVADDNSLLIAGTTSTDFTIYAATGTSTVSTPSGQPMWLAKFDSDGGLLNAIAIGG
                     PSGSSAPYPVSTSVDNSGNATITGYYTGNVDFDPGVGATTLSGSLSDAYGFIAKYSAT
                     GNLLWAKKIGNGSNTCSVLDHAVDNAGNIILGGWFTQTVDFDLDPFVTHALTAVGSGD
                     AFFAKYDADGNYIWAGQLVENLFYGGVNEIETDAGNNIYVSGFMDKPCDFDIGPAVKT
                     RKPHGTGFYVAKYTSSGALKWIRQPSGNGDFLLNEMYVDPAGNVFTSGTFSGTFDFDF
                     STGTYNLSGPDVDLLITDQWLSCMDKSGNFNYAFSLHTDPTVISGISSIYSVCVTTDN
                     KLIIAGKYLKTLDIDPGVPVWEISVPGVTEAGFVIGYDQPVFRVGDIEISTTLQVYPN
                     PADREITLVLGDVASADISIIDMQGKLIYHTGYNSDEEASIDVSAIPEGIYILRAKTN
                     DQIKSSTICIAH"
     gene            complement(2122..3024)
                     /locus_tag="PLL28_12990"
     CDS             complement(2122..3024)
                     /locus_tag="PLL28_12990"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNF70288.1"
                     /translation="MQLHSHISKHKLPILLFAGICLLSACYTNKLSKLAPTEQYPDDT
                     WLSQQTNKRALIIVAHDDDALGCAGTITQLCEQGWQVSMFCLYPDISRVADRNAQRQI
                     DIKNAIAIQGIQDIRWHEFIYRKDLETSQTAYYPVPESSFDTLFFTDTLLSLINAHIE
                     DWKPSVIFTLDDQIGGYGHPEHKYISMLALRCAKEHQLAKGGTVERVYQAVFSPSQAE
                     TSLKDSEVYQAAKEVYHCQGMPIPTTQINIQSAADKKYRAMQAYTTEQPNIMRVWLFY
                     NKVDADTYFNIFDREFFRVIETGE"
     gene            complement(3054..3590)
                     /locus_tag="PLL28_12995"
     CDS             complement(3054..3590)
                     /locus_tag="PLL28_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNF70289.1"
                     /translation="MQILIIILAAIAGVVALIFIIASFSSSNFQIVAQLPIKRNRADI
                     YNYVKFLRNSDYFNKWVMMDPGMEKKYTGTDATVGFTYKWKSENKQLGEGEQEIIGME
                     ENSYISYVIRFIKPFENTSQSTILLTDLEPGTTMVSYTFTGQRNLMMKVVHLLFKLEK
                     ALQKDLMESLNNLKKVLE"
     gene            4169..5035
                     /locus_tag="PLL28_13000"
     CDS             4169..5035
                     /locus_tag="PLL28_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70290.1"
                     /translation="MKVRWDIDQIDEYTRKKQFSQLEEYKDNCAAFSNSFTEPVSERW
                     TMHDTMEYHTFKELREMYQAGETNVIVVALARIKITKGADDKKQFDLSYSYYGDEDAK
                     DSSGFDPNDFKALVVFPIEQIMELPDAFKKIYVGVQLEHQNPSKEELYYAVDVVTDLV
                     KRKAADNTFTDKVIKQSAPKLKDYTLAISKDDWDEDYKIKDAEPYYPLPIEVMEPAKF
                     KQLITEGKPGYAYAIKVGLNTTIMLSDKREVAYLQQPNDIPTRKASDKFANRDFHKIY
                     EAVTGVKVEFKE"
BASE COUNT         1571 a         1011 c         1111 g         1581 t
ORIGIN      
        1 tgaagtatca tctgcaaaag tgatcactga taagtttaca ggtaaatcac gtggcttcgg
       61 cttcgttgaa atgcctaacg acggtcagaa agccatggat gaactcaatg gcgcagagat
      121 catgggtaaa actattgctg tttctgaggc tcgtcctcaa actgagcgca aaccgtttaa
      181 caacaaccgc ggtggcggtg gtggtaacta cggcggaggc ggcggtggcc gtcgctggta
      241 atcagcataa gcttttcaaa aaaaaggcag ttgttaattc aactgccttt tttgttttgt
      301 gcgaatcgca gaagtattaa tgcgcaatgc aaatagtgct tgatttaatt tgatcattcg
      361 tctttgcacg taggatataa attccctccg gaatagctga tacatctatg cttgcttctt
      421 catcactgtt ataacctgta tgatatatca atttaccttg catatctata atcgaaatgt
      481 ctgcagaagc tacatcaccc aacaccaagg ttatttctct atctgcaggg ttaggataaa
      541 cttgtaatgt tgttgaaatt tctatatcac cgactctgaa taccggttga tcatagccaa
      601 tcacaaatcc ggcttcggtt acgccgggta cgctgatttc ccacaccggt actccgggat
      661 ctatgtctaa agtttttaaa tattttcctg caatgatcaa tttgttatct gttgttacac
      721 aaacgctgta tatagaggat attcctgaga ttaccgttgg gtctgtgtgc aacgaaaaag
      781 cataattaaa atttcctgat ttatccatgc acgacaacca ttggtcggtg atcagaagat
      841 ctacatctgg tccggataaa ttataagtac ctgtgctgaa atcgaaatca aaagttccgg
      901 aaaaagttcc cgaagtgaat acattacctg caggatcgac atacatttca ttcaacaaaa
      961 aatcgccatt gcctgatggt tgtctgatcc atttcaaggc accggaagaa gtatattttg
     1021 caacataaaa tcctgttcca tgcggctttc gggttttaac tgcgggaccg atatcaaaat
     1081 cgcagggctt atccataaat cctgatacat aaatattgtt tcctgcatca gtttcaattt
     1141 catttacgcc accataaaac aaattttcaa ccagttgacc tgcccatatg taatttccat
     1201 cagcatcata ttttgcaaaa aatgcatcgc ctgagccgac tgcagtaagt gcatgggtaa
     1261 caaatggatc aagatcaaaa tctacggttt gagtaaacca acctcccaaa ataatgttac
     1321 cggcattgtc aacggcatga tctaatactg aacatgtatt cgatccgttt ccaatttttt
     1381 tcgcccacaa taaattacct gttgcgctat attttgcaat aaatccatag gcatcactca
     1441 gacttccact tagtgtggtt gccccaactc ccggatcgaa gtccacattg ccggtgtaat
     1501 accctgtaat ggttgcatta ccgctattgt cgacactggt tgaaacagga tagggggcgc
     1561 tggaaccgct tggaccacca atagcaatcg cattgagcaa tccgccatcg ctatcaaatt
     1621 ttgctaacca cataggctgg ccggaaggag ttgaaacagt tgatgtgcct gtggctgcgt
     1681 aaatggtaaa atcagttgat gtagttcctg ctatcaaaag gctgttatca tctgcaacaa
     1741 acgctgcttt tgattcgaga atagtagcct gttcgatatg cgccgaccag agtaattcga
     1801 aactgcttgt atacttggca ataagtactt gctggtttgg aggtccgtaa gtgagtacac
     1861 cggcacctgc atccaggtct tgcggataat agcttttatt gactagatag aagtttcctt
     1921 catggtcggg atatacccca aatttgctgt tgtaatcagt ctgccccgga ttcaacactg
     1981 cataaggcgt ttgggcttta agctgttgaa ttaacatgat ggcagtgaag aaaacgatgc
     2041 atatcggttt cataaggtgt gtatttgtat tgaaaattac acctttttat atgctgatgg
     2101 aaatttttgt aagattatta tttattcccc ggtttcaatc accctgaaaa attcgcgatc
     2161 gaaaatgtta aaataggtgt cggcatccac cttattgtaa aataaccaaa cgcgcataat
     2221 attaggctgt tcggtggtat atgcctgcat agcacgatat tttttatcgg ctgcggattg
     2281 gatattaatt tgagtggtag gaataggcat accctgacaa tgataaactt ctttagcagc
     2341 ttgataaacc tcactgtctt ttaacgatgt ttctgcctgc gatggtgaaa aaactgcctg
     2401 gtaaacgcgc tctaccgtgc ccccttttgc aagttgatgt tcctttgcac aacgcaatgc
     2461 cagcatgctg atgtatttat gttccggatg accgtagcca ccgatctgat catctaaagt
     2521 gaaaattacg gaaggtttcc aatcttcaat atgtgcattg atcaatgata ataatgtatc
     2581 agtaaaaaat aaagtatcaa atgaagattc cggaacagga taataggctg tctgacttgt
     2641 ttcaagatcc ttgcgatata taaattcatg ccacctgata tcttgaatgc cctggatggc
     2701 gatagcattt tttatatcaa tttgccgctg tgcatttcga tctgcgactc tggaaatgtc
     2761 gggatacaaa caaaacatgc ttacttgcca gccttgttcg caaagttgag tgatggtacc
     2821 tgcgcaacct aaggcatcat cgtcgtgtgc tacaataatt aatgcacgtt tatttgtttg
     2881 ttgtgataac caggtgtcgt caggatattg ctctgttggg gctagtttgc ttaatttatt
     2941 cgtatagcag gcacttaata aacagatgcc tgcaaaaagt aaaatcggca atttgtgttt
     3001 agaaatgtga gaatgcaact gcaagcttta ttttttgaga tttacaatgt tgcctattcc
     3061 aacactttct tcaaattatt caatgattcc atcaagtctt tttgcaatgc tttttccagt
     3121 ttaaagagca agtgtactac tttcatcata agatttcttt ggccggtaaa tgtatagctt
     3181 accattgtag ttccgggttc gagatcagtt agcaggatgg tagattgcga agtgttttca
     3241 aaaggtttaa taaagcggat cacataactg atatagctat tttcctccat accgatgatc
     3301 tcttgttcac cttcgccaag ttgtttgttt tcacttttcc atttgtaggt gaatccaacc
     3361 gttgcgtcag tgccggtgta ttttttttcc attccaggat ccatcattac ccatttatta
     3421 aaataatcgc tgttgcgaag aaatttcaca taattgtaaa tgtctgccct attgcgtttt
     3481 attggcaatt gagcaacaat ctggaagttt gaagatgaga aggaggcaat gataaagata
     3541 agcgcaacaa cgcctgctat tgctgcaaga atgattatta gaatttgcat gactttgagt
     3601 atttgaatac caaaggtaag caatcggatg aaaaaaaagc agcatgagtg atatgcggct
     3661 ttttgtatgg aatatgctat tttggattct cttagcttgt gtaaagatta tcagctgagt
     3721 ttaagattat tatcctttta tcattaaata ttcttacatc tcgctttagc atgtaagcta
     3781 tgaagtgata gtgttcaccg gtcaaaaatg ctttgagcct ttcggttgga atcccattgg
     3841 atttgaagta aagtattata ttccgggtat taaatttggc atatttaggc gcgtgtttgg
     3901 cgttttttag gttaaatgaa gcaatttagc gtggaaattg tccaacatcg gcaaattatt
     3961 tctctttgtg atattcaaat gaaaacctaa ttttgaacca aacagcaaga ctatgcgcat
     4021 cggaagattt ttacccttac tattgatcct tacctttttt cattataaca ccgcaaatgc
     4081 ccagttcggt agccctctga ccgtttttaa aaacgatctg cccacccaaa aggaatgtgc
     4141 cgagataaca ggccgaaaac tggttgttat gaaggtacgc tgggatattg accagatcga
     4201 cgagtatacg cggaaaaaac aattcagtca gcttgaggaa tacaaagaca actgtgcagc
     4261 tttcagcaat agtttcacag aaccggtaag tgaaaggtgg actatgcatg atactatgga
     4321 atatcacaca ttcaaagagt tgcgcgaaat gtatcaagcc ggggagacaa acgtaattgt
     4381 tgttgcattg gcgcgtataa aaatcacaaa aggtgctgat gataaaaagc agtttgattt
     4441 aagctattca tattatggtg atgaagatgc caaggattcc tcgggttttg acccaaacga
     4501 ttttaaagca ctagttgtat ttccaatcga acaaattatg gaattacctg atgctttcaa
     4561 aaagatttat gtaggcgtgc aattggaaca tcaaaaccct tcgaaggagg aattgtatta
     4621 tgcggttgat gtagttactg atcttgtaaa acgcaaagcc gctgacaata catttaccga
     4681 taaggtgatc aagcaatcgg ctccgaaatt gaaagattat acccttgcca tttcaaaaga
     4741 tgattgggat gaggattata aaataaaaga tgcagaaccg tattatccgc tgccaatcga
     4801 agtaatggag cctgcaaaat tcaagcaatt aattactgaa ggaaaaccag gctacgctta
     4861 tgcgattaag gtaggactca atacaaccat catgctttcc gataaacgcg aagtagcata
     4921 tctgcaacaa cctaacgata tcccaacacg caaagcctcc gacaaattcg ccaaccgtga
     4981 ttttcataaa atatatgagg cggtgacagg ggtgaaggtg gagtttaagg aatgataaag
     5041 gaaagaggaa agaggagaga ggagagaggg gtgagtgggt gagtgggtga gtgggtcagt
     5101 gggtgagtgg gtgaggttac cgcgcagaga aggcggctgg gtcagtagga aaataaaatg
     5161 ggctaagact ttgtcttagc ccattttatt ttaaaaaaaa atcccgaccg caggtcggga
     5221 aaaatacttg atacttgata cttgatactt gatacttgat accactcacc actc
//
LOCUS       DAOQHY010000204         5257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_8924_length_5257_cov_5.739715, whole genome shotgun sequence.
ACCESSION   DAOQHY010000204 DAOQHY010000000
VERSION     DAOQHY010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8924_length_5257_cov_5.739715"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..452
                     /locus_tag="PLL28_13005"
     CDS             60..452
                     /locus_tag="PLL28_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70291.1"
                     /translation="MLKRKMQGKIKQEILDIETTNIKLQRAGWPETPLSPSEMSNVLG
                     VDGVISSNYSMSKPMSEGGAIALSLLTGAYGATNEVNVTMSIYDRNTSTLIWNFDHKY
                     SGGIGSSPCTLVDELMRYASKKMPYLVY"
     gene            646..2079
                     /locus_tag="PLL28_13010"
     CDS             646..2079
                     /locus_tag="PLL28_13010"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNF70292.1"
                     /translation="MQPDRLSFRGQLLYVTGTFGWSVVVNLHAGMILYFYQPVGDPAL
                     QNLIPKLFIFGLLPVLALVVFSARLVDALVDPVIAHISDGSNHKKGRRIPLMTYALIP
                     TLITGILLWIPPTRGESVANLYWLGIIQILYNIAISFYVIPYNALLPEFGYNAETKLR
                     LSTFQSIAYTIGLVAASASNALVNFYQDVMHVDDKFRAFQFAIWTIFLIGWASLLLPV
                     FINEKKYIIPNRVTKSLNENFKTVLRNKNLVIYLTADFTYFISLTIIGSGALYYVKAL
                     LHIEEKHGTGMVATAVGLAMLWSPGVYYLARRVRKKILVLFSLIALSLVFGSVRWLGH
                     FGMDNVSEAYVLAAVISVPFAFLGILPPVILAELTQADAHITNENREATFFAIRSVFI
                     QIGQTLGIVIFTILIGMDQSQGLGKYFSGVFHNIPFEELGIRLSGIFGFGLGLLAAIF
                     FFFFDDKQLNKTIKEMEDQHEKKAMEE"
     gene            complement(2080..2607)
                     /gene="purE"
                     /locus_tag="PLL28_13015"
     CDS             complement(2080..2607)
                     /gene="purE"
                     /locus_tag="PLL28_13015"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNF70293.1"
                     /translation="MAKQQTAKKLPPMVSIIMGSDSDLPVMRDAAEVLESFGIPVEVT
                     VVSAHRTPQRMVQFASKAHTRGIRVIIAGAGGAAHLPGMVASITPLPVIGVPVRSSNS
                     IDGWDSILSILQMPNGVPVATVALNAAKNAGILAAEIIGSSDKTILNKIVAYKNEMRE
                     AVEKKAKKIEKDGYR"
     gene            complement(2637..3791)
                     /locus_tag="PLL28_13020"
     CDS             complement(2637..3791)
                     /locus_tag="PLL28_13020"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HNF70294.1"
                     /translation="MNSVETSKLELGILGGGQLGKMLIEAASDWNIRCHVLDPDPQAS
                     CSKLAHTFVNGSFADYDTVYQFGKNLDRLTIEIEHVNVAALHQLEKEGKQIFPQPAVL
                     EIIQDKGKQKAFYEYNDLPTSNYQYVDSKEALIELLEEEKLSYPFVQKTCKAGYDGKG
                     VAVIKSKDDIVSLLDGPCIIEDVVDIEKELSVIVARNRNGNTSCFPTVEMEFHPVANL
                     VEFLLAPANISEAQEETAKQIAIDCINAFGMTGILAVEMFLTRNGEILINEVAPRPHN
                     SGHHTIEANDVSQYQQLLRCLFDLPLGSTKAWQHAIMVNLLGEPGYSGLAKYEGLSDC
                     LSLEGVHVHLYGKQITKPYRKMGHVTIIGDNIQAMREKASFVRERLRIIA"
     gene            3856..>5257
                     /locus_tag="PLL28_13025"
     CDS             3856..>5257
                     /locus_tag="PLL28_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70295.1"
                     /translation="MKTQTRIFIIAIVLACAQINAFAQPCAFFEDYTSDAGWTDYYYY
                     YAVTGACLSDEQDGSLTFGGGKLNFNALDDANDTRIYFSLPTPAYEDFWTVSGEFTPT
                     AGGASGRTGSMIVALSNGSDNPISDTYSICEFSNTDAIALMWTSDFDAAPDNIGFEIW
                     ANDNGTVTMSERLIAPYGDTYYFQWTRIAIEYMSLDVYLDATHTDLFATIPCFYLPTT
                     ITDLNTMQSGNFPGGSYLRQLTATLDNVCLRNIDVTAASITGPETICLGSENDYTITT
                     FAGADIDWTLPAGITYTGDAAPTIHVTDWPGAGTYTLMSVINYNCFYDTAYYEVTVID
                     PGSGEVLEEDFCEGGSTTVDVTTTDATYLWDDGTTEGTHSYDTEGTYWVTIYIAGCEF
                     TDTIHITEYPNPSIDLGDDMDICGDVTVEGPSGFTSYEWNTGSASANLTTSIPGNYSL
                     TVTDENGCTAFDDITLN"
BASE COUNT         1488 a         1137 c         1046 g         1586 t
ORIGIN      
        1 gaatcattaa aggaacaaca gaaaactgag tcacttaatt ttcaaaagga aatgcttgga
       61 tgcttaaaag aaaaatgcaa ggtaagatta agcaagaaat attagacatt gaaacaacta
      121 atatcaaatt gcaacgtgct ggttggccag agacaccact ttctccttct gagatgagca
      181 atgttcttgg ggttgatgga gtaatttctt ctaattattc aatgtcaaaa cccatgtctg
      241 aaggcggtgc aattgccttg tcattattaa ctggcgctta tggcgcaaca aatgaagtta
      301 atgttacaat gagcatttac gatcgaaaca cctcaacttt aatttggaat ttcgatcaca
      361 aatattctgg aggcatcgga agctccccgt gtactttagt agacgaatta atgcgttatg
      421 caagcaaaaa aatgccttat ttagtgtatt aatgcacttg aaattataaa caaacgcagg
      481 tcaaattttt agttttggat taccattacg ctcaaaatat taagtttcaa taaaaacaaa
      541 ttatacatat gcacaccttc tgtgcagaac cgacaggcga ataccctgtc ggtttttttg
      601 tgccctcatc tattatccta cctttgcatc ctcaaggatc agtatatgca gccggatagg
      661 ctatcgttta gggggcagtt gttgtatgtt acaggcactt tcggttggag cgtggttgta
      721 aacttgcatg ccggtatgat tttgtatttc tatcagcctg ttggtgatcc tgcccttcaa
      781 aacctcattc ccaaactttt tattttcggt cttctgccgg tattggcatt ggtggtattc
      841 agtgcaaggc tggtagatgc acttgtagac cctgtaattg ctcatatcag cgatggcagc
      901 aatcacaaaa agggacgtcg aattccctta atgacctacg ccttaattcc taccctaatt
      961 accgggattt tgctttggat acctccaact cgtggcgaaa gtgtcgcaaa tctttattgg
     1021 ctgggtataa ttcagatatt gtataatatc gcaatctcgt tttatgttat tccttacaat
     1081 gcgttgcttc ctgaattcgg gtataatgcc gaaacaaagc ttagactttc aacctttcaa
     1141 tcaatagcat ataccattgg tcttgtagca gcaagtgcct caaatgcctt ggtgaatttt
     1201 taccaggacg tgatgcatgt tgatgacaaa tttcgcgcat ttcagttcgc catctggacg
     1261 atctttctga ttggatgggc cagtttgtta ttgccggttt tcatcaatga aaaaaaatac
     1321 atcatcccca atcgtgttac aaaatcactg aatgagaatt ttaaaacggt attgcgaaac
     1381 aaaaatcttg tcatttatct cactgccgat tttacgtatt tcatctcgtt aaccattatt
     1441 ggttccggag cgctttacta cgtaaaagcg ttattacata tcgaagaaaa acacggcacc
     1501 ggaatggttg ccacagcagt cggtcttgcc atgctatggt cgccgggagt atattatctg
     1561 gctagacgtg tcaggaaaaa gattctggta cttttctccc ttattgctct ttctctggtt
     1621 ttcggatcag tgcgctggct cgggcatttc ggcatggata atgtttctga ggcctatgtt
     1681 cttgctgcgg taatttcagt gccctttgca tttttaggta ttctcccccc tgtaattctt
     1741 gccgagttga cacaggccga tgcgcatatt accaatgaga acagagaggc tacctttttt
     1801 gcgatcagaa gtgtatttat tcaaattggt caaacactag gcatagtgat ttttaccata
     1861 cttatcggta tggatcaatc tcaaggttta gggaaatatt tttcaggtgt atttcacaat
     1921 attccattcg aagaattagg cattcgccta tcgggaatat ttggcttcgg attgggttta
     1981 cttgctgcca tctttttctt cttttttgat gacaaacaat taaacaaaac cattaaagaa
     2041 atggaagatc aacacgaaaa gaaagcaatg gaggaataat cagcgatatc cgtctttctc
     2101 aatttttttt gccttctttt ccaccgcttc acgcatctca tttttataag caacaatttt
     2161 attgagtatg gtcttatcac tactgccaat aatctctgca gccaatatac cggcattttt
     2221 tgcggcattg agcgctacag tggcaaccgg cacaccattt ggcatttgca aaatactcaa
     2281 tatggaatcc caaccatcaa tggaattgga agaacgcaca ggaactccaa taaccggtaa
     2341 aggggtaatc gacgcaacca ttcccggcaa atgcgctgca ccacctgctc ctgcaataat
     2401 tacgcgtata cctctggtat gtgccttgga ggcaaattgt accatgcgct gcggggtgcg
     2461 atgagcagaa actaccgtaa cttcaacagg aattccaaat gattctaata cctcagcagc
     2521 atcacgcatt accggaagat ctgaatcgct acccataata atgctaacca ttggaggtag
     2581 ttttttcgcc gtttgttgtt ttgccatggt gtaaagttac aatttatata tagcccttat
     2641 gcaatgatgc gtagtcgctc tcttacaaag gatgctttct cacgcatagc ttgaatatta
     2701 tcaccaataa tggtaacatg ccccattttg cgatatggtt ttgtgatttg cttgccatat
     2761 aaatgcacat gaacgccctc taacgacaaa caatctgata acccttcata ttttgccaag
     2821 cctgagtaac cgggttcgcc aagcaagttt accatgatag catgttgcca tgctttggtg
     2881 cttcccaatg gcagatcaaa cagacatcgc aataattgct gatactgtga aacatcattg
     2941 gcttctatcg tatgatgccc gctgttatgt ggacgtggtg ccacctcatt gataagaatc
     3001 tcaccattgc gtgtaaggaa catttcaact gctaatatgc ctgtcatacc aaatgcattg
     3061 atacaatcta tggcaatctg tttagcggtt tcttcctgtg cttcggaaat atttgcaggt
     3121 gcaagcaaga attctacaag attggcaacc ggatgaaact ccatctcaac agtaggaaaa
     3181 catgaagtat ttccattccg gtttctcgct actatcacag ataattcctt ttcaatgtca
     3241 acaacatctt caatgataca tggcccatcc agtaagctga caatatcatc cttgctttta
     3301 attacggcaa cacctttacc atcataaccc gccttacatg ttttttgcac aaatgggtag
     3361 ctgagttttt cttcttccaa taattcaatc agcgcctctt tactatcaac atattgataa
     3421 ttactggtgg gcaaatcatt gtattcataa aatgcctttt gtttgccttt atcctgaata
     3481 atttctaaca ctgcaggttg aggaaatatt tgcttgccct ctttttccag ctgatgcaat
     3541 gcagccacat tcacatgttc aatttctata gtcaggcgat cgagattctt gccgaattga
     3601 tatacagtat cataatcagc aaaactcccg ttaacaaagg tatgcgccag tttactgcaa
     3661 gaagcctggg gatccggatc aagtacatga cagcgaatat tccagtcact ggcagcctcg
     3721 ataagcatct tgcccaattg ccctccacca agtattccaa gctccagctt gcttgtttcc
     3781 acagaattca tggtgcaaag gtacggacat ttcattcagc aggaaaaggg tacctttgtt
     3841 gttcaatcag gcacaatgaa aacccaaacc cgaatcttca ttattgccat agtattggca
     3901 tgtgcgcaaa ttaatgcttt cgctcagcct tgcgccttct ttgaagatta cacctctgac
     3961 gcaggttgga ccgactacta ctattactat gctgttaccg gagcatgcct tagcgatgaa
     4021 caggatggct cgctcacctt tggaggtggc aaactgaatt ttaacgccct tgatgatgca
     4081 aacgacaccc gtatttactt ttcactgccg actccggcat atgaggattt ctggacagtc
     4141 agcggagagt ttacccctac agcaggagga gcaagcgggc gcacaggttc tatgatagtt
     4201 gcgctttcaa atggatctga caaccctatt tcagatactt attcgatttg cgaattttca
     4261 aatacagatg ccattgcact tatgtggacc tcagattttg atgccgcacc tgataatatc
     4321 ggatttgaaa tatgggcgaa tgataatgga acagtcacta tgagtgaacg acttattgct
     4381 ccttatggcg atacctacta ttttcaatgg acacgcattg caatagaata catgtcgttg
     4441 gatgtttacc tggatgcgac acataccgat ctgtttgcaa ccattccctg tttttatttg
     4501 cctacaacta tcacagacct aaataccatg caatcgggga acttccccgg cggttcatat
     4561 ttacgccagt taacagctac tttagacaat gtttgtttgc gaaatatcga tgttaccgca
     4621 gcaagtatta caggaccgga aacaatttgt ttaggtagtg aaaatgacta caccatcacc
     4681 acatttgcag gagcagatat tgattggact ttgccggcag gaattaccta taccggcgat
     4741 gcagcaccaa ccattcacgt aactgattgg cccggtgccg gaacatacac actgatgtca
     4801 gttatcaact ataattgttt ttacgatacc gcatattacg aagtaactgt aatcgatccg
     4861 ggaagcggcg aagtgttgga ggaagatttt tgtgaaggag gaagtacaac cgttgatgta
     4921 actactactg atgcaaccta tttatgggac gatggcacaa cagaaggcac acattcttac
     4981 gataccgaag gaacctattg ggtaaccatc tatatcgcag gatgcgagtt tacagatacc
     5041 attcacatca cagaatatcc aaatccatca attgatctgg gtgatgatat ggatatatgt
     5101 ggagatgtaa cagttgaagg tcccagcgga tttacgagtt acgaatggaa taccggtagt
     5161 gcgtcagcga atttgacaac ttccataccc ggtaattact cactgactgt tacagatgaa
     5221 aatggatgca ctgccttcga tgacattact ttaaatg
//
LOCUS       DAOQHY010000205         5159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_9199_length_5159_cov_6.508425, whole genome shotgun sequence.
ACCESSION   DAOQHY010000205 DAOQHY010000000
VERSION     DAOQHY010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9199_length_5159_cov_6.508425"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PLL28_13030"
     CDS             <1..226
                     /locus_tag="PLL28_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70296.1"
                     /translation="VVFVEQIRGEKKEKGKRRKEKGERKKERGEWMSLPRRAGGWVSD
                     KQIRLQSERKIALVSLLNTHYSLPVVNRDS"
     gene            403..813
                     /locus_tag="PLL28_13035"
     CDS             403..813
                     /locus_tag="PLL28_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HNF70297.1"
                     /translation="MQYWLIKSEPEAYSWDDLVEKKIDIWSGVRNYAARLHLRAMKKG
                     DLCLFYHSGNVSACVGIAKVVKAAYPDPTAEEGDWSAVDVAPVKKLKREVSLKEIKLI
                     PALADMPLVRISRLSVSPVTEFQYHKILDMSNDK"
     gene            complement(878..2047)
                     /locus_tag="PLL28_13040"
     CDS             complement(878..2047)
                     /locus_tag="PLL28_13040"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNF70298.1"
                     /translation="MQFYERKYRLKLILVLVAMVIVAATLLYTNNLATKLAEEERQKV
                     TQIADVYHYIATANDNTDFRFFIELIQANKTVPIISTDNDDNIVAFLNIDSAKVAKDT
                     TYLKKRLEEMKDFAEPIKLELSAGIYQYVYYQHSDLYQQLLYYPYVQLMIIAAFLIVA
                     YTLFNIARRSEQNRVWVGMAKETAHQLGTPISSLAAWVEYLENIPELDGRKHVMEEMD
                     KDVQRLVLIADRFSKVGSQPDLQPCHLGDELARSIDYIKRRSSQKAVFNFEQSDSDII
                     VNMNVILFNWVIENLLKNALDAMEGVGEITVEIRTENDQAIIDISDTGKGIPRSKQKT
                     VFEPGYSTKKRGWGLGLTLAKRIVESYHKGKIFVKTSIPGKGTTFRIQLPLSNTK"
     gene            complement(2102..4735)
                     /locus_tag="PLL28_13045"
     CDS             complement(2102..4735)
                     /locus_tag="PLL28_13045"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HNF70299.1"
                     /translation="MQETNPSAVPHQDTPKPEKPRRKKILKYIFVGFGIFIGLILLIA
                     ILVPVLFEDKIKALFVAELNKSLATPVTINEDDIHLSLFQHFPEASLIFNNVGIRESF
                     PKSQKNFLEAKEISLVFNFWNILKSNYTIKNIVVKDGYCRLITDKRGNINYKFWKESD
                     DTTSSDFQIQLDRVECMQMDMQYIDYKYGQDVDLQLHDCTLSGNFSSDLYTMTATGDL
                     LSRRIRVGSANYLVNKEAHVDTKLLVNVPEESYTFEGGKITVEENTFLLDGSIALKGD
                     DMYDLTINGDEIDLAGLMLLLPGNISGNLESLETKGDINFAATIKGPYTATKDPAINI
                     DFSVRDGSIKHEKFGGRLDNMAFTGKYTNGSKQQSATSSISIKDFSASRDGNPLEMAL
                     VYSNFVNPFIDLQLNGELPASLIIPLALPDVKDADGMIKLNNINIKGNIKSFNADQGQ
                     APPTGTITFEGVSCTMNGEHVEIPAGVAVVSNNELSLQQIKLSAAGSNLLADLTIGNW
                     IQHVFPSAISPALNINGSIQAENFDVTGLLTLLGNESDTEVTTAPTTATATSTSTTSD
                     PMNISGQVDLKCGRLTYDQLSLEDVKTTVKLSPGFIVLNDMSGNTMNGKFIMNTSFRT
                     LPTGELLLQTSGQLIAIDVRELFRQLDNFDQSYLTDKNLKGSITGNIYDVAVKWNADY
                     SLDEQSIYALCDMRIENGELIEYKALESLSAFVKLSDLRHIKFSTLENKIEIANRQVK
                     LPAMQIKSNAMDLYMSGTHSFDNVIDYQFRISLADLMVRKFLGGNKQQDNYETDAEGG
                     VNVYVSMTGTVDDPILKYNKREAKQKLQDSGLEQQRFIDIFKRDPEETMFHETETPEV
                     KPGETDTEDIEFIEFDEEEDK"
     gene            complement(4852..>5159)
                     /locus_tag="PLL28_13050"
     CDS             complement(4852..>5159)
                     /locus_tag="PLL28_13050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNF70300.1"
                     /translation="TLSDTIEITECIGLGIPTAFSPNNDGTNDQFMVVTLHPEDILSF
                     NIYNRWGQLLFSGDAAHYAWDGTFNNTPQPMGSYVYVLHFLNDGVEEVLQGNFTLVR"
BASE COUNT         1463 a         1030 c         1067 g         1599 t
ORIGIN      
        1 agttgttttc gttgagcaaa tcagggggga aaaaaaggag aaaggaaaaa ggagaaagga
       61 aaaaggagag aggaaaaagg agagaggaga gtggatgagc ttgcctcgcc gagcaggcgg
      121 gtgggtgagt gataagcaaa ttcgcttgca aagcgagcga aaaattgcat tagtctcact
      181 actcaatacc cactactcac taccagtcgt gaatcgtgat tcgtgactcg tgagtgtggt
      241 gcgtacataa ggatcagaga tgtgattcga gtatgcattt acctgatacc tgatacttga
      301 tacctgatac ctgaaacctg atacaaattc taacacccgc ctggagcgaa gcaagcttga
      361 ctcccggtac tcaattctcc cactccatcc ttatatttgc atatgcaata ttggttaata
      421 aaatcagaac ccgaagctta ttcgtgggat gatctggtag agaagaaaat tgatatttgg
      481 agtggtgtgc gcaattatgc tgcacgattg catttgcgtg ccatgaaaaa aggtgatttg
      541 tgtttgtttt atcatagcgg aaatgtttct gcctgcgtag gaattgcgaa ggttgttaaa
      601 gcagcatacc ctgaccctac tgctgaagag ggcgactgga gtgccgtaga tgttgctccg
      661 gtaaaaaagt tgaaacgtga agttagtttg aaagaaatta aattgattcc tgcacttgca
      721 gatatgccct tggtgcgaat tagcagattg tctgtatcac cggttactga atttcaatat
      781 cataagatat tggatatgtc gaatgataaa tgaagataat gttttagcag tttgccgtaa
      841 tccggaagat caaaacgctc aaacctttca ttaattttta cttcgtatta ctgagtggca
      901 attgtatccg aaaggttgtg ccttttccgg gaatagatgt ttttacaaat atttttcctt
      961 tgtgatagct ttcaacaatg cgttttgcca gtgtgagtcc taatccccac ccacgttttt
     1021 ttgtagagta tccaggttca aaaacagttt tttgttttga gcgtggaatg cctttgccgg
     1081 tatcggagat atcaataatt gcttgatcat tttctgtgcg tatctcaaca gttatctcgc
     1141 caacaccttc cattgcatca agtgcatttt taagtagatt ttcaatcacc cagttaaaca
     1201 gaatcacgtt catgtttacg atgatgtcac tatcagactg ttcgaaattg aatacagctt
     1261 tctgtgatga gcgacgtttg atgtaatcaa tagaacgtgc taattcatcg ccaaggtgac
     1321 agggttgcag atcgggctgc gaacctactt ttgaaaaacg atctgcaatc aatacgagtc
     1381 gctgcacgtc tttgtccatt tcttccatca catgcttgcg accatctagc tcaggaatat
     1441 tttccaggta ttctacccaa gctgccagag aactgattgg tgttcctaat tgatgtgcag
     1501 tctctttggc cattcccacc catactctgt tttgctccga acgccgtgca atattgaaaa
     1561 gggtatatgc tacaattaag aatgcggcaa tgatcattaa ttgcacgtac ggataatata
     1621 ataattgctg gtagagatcg gaatgctgat aatacacgta ttgatagatg ccggcactta
     1681 attccaattt gatcggttcg gcaaagtcct tcatttcttc aagacgcttt ttcaaatacg
     1741 tagtatcctt ggcaacttta gcggaatcta tattcagaaa ggctacaata ttatcgtcat
     1801 tgtcggtaga aataatggga actgttttat tggcttggat caactcaata aaaaatctaa
     1861 aatccgtatt atcattcgct gtggcgatat agtgatatac gtcggctatt tgcgtaacct
     1921 tttgccgttc ttcttctgcg agcttggtag cgagattgtt tgtatacagc agagtggcag
     1981 ctacaataac catagccacc aatacgagta ttagtttcag tcgatactta cgttcgtaaa
     2041 attgcatgct cacggtatcg gctaagttaa tgggtttctg ataaattgtt gaacagcact
     2101 gctatttgtc ttcctcttcg tcaaattcta tgaattctat atcctcggta tctgtttcgc
     2161 caggtttcac ctcgggtgtt tcagtttcat gaaacattgt ttcttcagga tcgcgcttga
     2221 aaatatcgat aaagcgctgc tgctcgaggc cactatcctg aagtttttgt ttcgcctcac
     2281 gcttgttgta tttcaatatc ggatcgtcaa cggtacctgt catactcaca tacacattca
     2341 cgcctccttc agcatcagtt tcgtaattgt cctgctgttt atttccacca agaaatttgc
     2401 gcaccattaa atcggcgaga ctgattcgaa attgataatc aattacattg tcgaaactgt
     2461 gtgtgccact catgtataag tccatcgcat tggatttgat ctgcatggca ggaagtttca
     2521 cctgacgatt cgcgatttca attttatttt caagagtgga gaatttaata tggcgaagat
     2581 cactcaattt tacgaatgca gagagcgact ccaaggcttt atattctatc aactctccat
     2641 tttcaatacg catatcgcac aaagcataaa tagattgctc atcaagcgag taatcggcat
     2701 tccactttac tgctacatca taaatatttc cagtaatgct acccttaaga tttttatctg
     2761 tcaaatacga ctgatcaaaa ttatccaact gtcggaataa ttcacgcaca tcgatagcaa
     2821 ttaactgccc ggaagtttgc aataatagtt cacctgttgg caatgttctg aaactcgtat
     2881 tcatgataaa tttgccattc atggtatttc ccgacatgtc attcaacacg ataaatccgg
     2941 gagataattt cacagtggtt tttacatcct caagacttag ttgatcataa gtcaaacgtc
     3001 cgcatttcaa atcaacttga ccgctgatat tcatggggtc ggaagtagtt gaagtagatg
     3061 ttgctgtagc tgttgtggga gcagtagtaa cctctgtatc actttcgtta cctaataaag
     3121 tcagtaagcc tgtaacatca aaattttccg cttgaattga accattgata tttaaggcag
     3181 gcgatattgc tgatgggaaa acatgttgaa tccaattgcc aatagtaaga tcggcaagaa
     3241 gattactccc ggccgcagaa agttttattt gttgaagtga aagctcatta tttgaaacta
     3301 ctgccacacc tgcaggaatt tctacatgtt cgccattcat agtacaactc acgccttcaa
     3361 atgtaatggt gcctgttgga ggtgcctgac cttgatcggc attaaaggat ttgatatttc
     3421 ctttgatgtt gatattattc aatttaatca tgccatcagc atcttttaca tcaggcaagg
     3481 ctaatggaat aattagtgag gcaggcaatt cgccatttaa ttgaaggtcg atgaatggat
     3541 tcacgaaatt ggaatatacc aatgccattt caagcggatt gccatcgcgc gaagcgctaa
     3601 aatcttttat gctgatggag ctcgtggcac tttgttgttt gctgccattg gtgtacttac
     3661 cggtaaatgc catattgtcg agcctaccgc caaatttctc atgtttgata cttccgtcgc
     3721 gaacactgaa gtcaatattt atggcagggt cttttgtggc agtgtaaggc cccttgatgg
     3781 ttgcagcaaa gttgatatca ccttttgtct ccagagattc gagattgcct gatatatttc
     3841 cgggaagcaa cagcatcaat ccggcaagat caatttcatc gccgttaata gtgaggtcat
     3901 acatgtcgtc gcctttaaga gcaatgctgc catcgagtaa aaatgtattt tcttcaacgg
     3961 taattttacc accttcaaaa gtgtaacttt cctccggcac gtttactaat aatttggtat
     4021 cgacatgtgc ttctttgttt acaagataat ttgcacttcc aactctgata cgtcggctca
     4081 ataaatctcc cgtggccgtc atggtatata gatccgaaga aaagtttccg gaaagtgtgc
     4141 aatcgtgcaa ttgtaaatct acatcctgac catatttgta atcaatgtat tgcatatcca
     4201 tctgcatgca ttcaacacga tctaattgaa tttgaaaatc tgatgaagtg gtatcgtcag
     4261 attctttcca gaatttgtag ttgatgtttc cgcgtttatc ggtgatcaat cggcaataac
     4321 catccttaac tactatattc ttaatggtat agttactttt cagaatattc cagaaattaa
     4381 ataccaggct gatttctttc gcttcaagaa aattcttttg tgatttcgga aagctttcgc
     4441 gtatgcctac attattaaaa atcaatgaag cttcgggaaa atgttgaaat aatgaaaggt
     4501 gaatatcatc ttcattaata gtaaccggag ttgccagact tttatttaac tcggcaacga
     4561 ataaggcttt tatcttatcc tcaaacagaa caggcaccag aatggcgata agcagtatca
     4621 acccaataaa aatgccgaag ccgacaaaaa tgtattttag gatctttttg cggcgcggtt
     4681 tttccggttt cggtgtatcc tgatggggga cggctgatgg attcgtttcc tgcacggtca
     4741 aagttaagga tgtatgggat aacgaagttt acgcaacgaa tagttttatg gttgcgaaaa
     4801 attaaccaaa catgcctcgt agtcgagacc ctcgggggaa taatattccg attatcgcac
     4861 caaggtgaaa ttaccttgta acacttcctc aacgccatca ttaagaaaat gcagcacata
     4921 cacataactg cccatcggtt gtggggtatt gttgaaggtg ccatcccatg cgtaatgggc
     4981 tgcgtcgcca ctaaacaata actgtcccca tcgattataa atgttgaagg aaaggatgtc
     5041 ttcaggatgc agtgtcacca ccataaactg gtcgttcgtg ccatcattgt taggactgaa
     5101 ggcagttggt attccgaggc caatacattc ggttatctca atggtatcgc tcagtgtct
//
LOCUS       DAOQHY010000206         5122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_9291_length_5122_cov_4.452733, whole genome shotgun sequence.
ACCESSION   DAOQHY010000206 DAOQHY010000000
VERSION     DAOQHY010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5122
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9291_length_5122_cov_4.452733"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(323..2941)
                     /gene="alaS"
                     /locus_tag="PLL28_13055"
     CDS             complement(323..2941)
                     /gene="alaS"
                     /locus_tag="PLL28_13055"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNF70301.1"
                     /translation="MMTSREIRQQFLQFFERQQHKIVASAPIVVKNDPTLMFTNAGMN
                     QFKDLFLGNKPVQYPRIADTQKCLRVSGKHNDLEEVGVDTYHHTMFEMLGNWSFGDYF
                     KKESITWGWQLLTDIYKLDPSRLYVTVFEGDPSENLAFDTDAYDVWKSMISEAHILRG
                     NKKDNFWEMGDTGPCGPCSEIHYDMRGDAERSAIDGAALVNTGDPQVIEIWNHVFIQF
                     NRKADGSLEELPNKHVDTGMGFERLVRALQGKSSNYDTDVFTPLIRKVETLSGFQYGQ
                     DEKTDIAMRVISDHIRAVSFCIADGQLPSNNGAGYVVRRILRRAIRYGYSFLHLKKPF
                     FNELVAVLAEQFRDVFPELDAQRDLVSRVIMEEERAFLRTLENGIKRLDDVTRSEQVK
                     SAKIIPGAVAFELFDTYGFPLDLTMLIVREHGLDVDSAGFEAEMQKQKERSRQATGME
                     TGDWMVLRDDKSVDFIGYDHLQSESRVVKMRSVKMKNEMQYQVVLNKTPFYAESGGQT
                     GDTGTLSINTNIIRVLDTKKENDLIIHFTDQLPENTDAVVYAEVDARRRTDITYNHTA
                     THLIHAALRQVLGSHVQQKGSYVGPDRLRFDFSHFSKVSDEELRRIEQIVNAKIRENI
                     PVVTKVMPIAEAQQLGAMMLFGEKYGETVRVVIADENYSKEFCGGTHVGATGEIGYCR
                     LVNESAVAAGIRRIEAITGSAIEKYMDEQHELLKKIQDITKVSDVVKSVQSLADENAA
                     LKKEVEQFQLLQVQQVKASLLNAIETTGGMNIIRAQVDLGNADLIKKLAYELRNEVSD
                     LVCLLAADIQGKASLTLMISDNLVESKGYDAAKLIRELGKQIEGGGGGQKFFATAGGK
                     NTGGLRKALDSVTTLL"
     gene            3056..4030
                     /locus_tag="PLL28_13060"
     CDS             3056..4030
                     /locus_tag="PLL28_13060"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNF70302.1"
                     /translation="MAKKVKYFYNPANLRFEKHVTPRWVWTLRIIGWISTAAVFAFII
                     VWIAYTYFDSPKEQYLKNQISEMELELDFMNSKLDTMAVVLGEMTERDDNIYRVIFEA
                     EPVSRSERMAGIGGSERFQRMNKLDNAELLINTATKLERIRRQMVIQSKSFDEISELV
                     SNKEQLLSHTPSIQPVSNKDLERIASGFGWRIHPIYKFAKFHEGLDFTAPKGSDVFAT
                     ADGTVVLADNSQSGFGNCIVIDHGYGYKTRYAHLSAFKVRAGQKIKRGELIGLVGNTG
                     LSTAPHLHYEVEKNGTKIDPINFFYNDLSAEEYARMIEIANRSNQSYD"
     gene            4170..4505
                     /locus_tag="PLL28_13065"
     CDS             4170..4505
                     /locus_tag="PLL28_13065"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNF70303.1"
                     /translation="MPYKEKEIEKVYWSIGEVAEMLDLSFAQIRLWEKEFDVFQLKKN
                     KKGDRYFTKKDIEQLKLIKYLLKEKKYTIKGAREQLKSKKPQTDSRFQTIEALKKIRQ
                     FLVELKEQI"
BASE COUNT         1497 a         1159 c         1036 g         1430 t
ORIGIN      
        1 cagattcaaa tcagccaatg tatctgcaga aggattaaac agaccttgaa tatcatagtc
       61 cattttatat cgttcttcca atgatggcaa tgtcattttc agatcgctga gactttgggc
      121 actaccaatt gatagcacac ctataaaggt gactgaaaat gcgaatagcc tgaacatgga
      181 aatatcaaat tttaatcaaa attaacctgc aattggcgct ttcgcaaggt ttcgatgtca
      241 caaatttgcc aaatccacaa tatttccacg attaaaaagc gcttgaaatc gggaaaactt
      301 gcagtaattt gcctgataaa acttacagta acgttgtcac agaatccaat gccttcctca
      361 atcctcctgt gtttttacct ccggcagtag caaagaattt ttggcctccg ccgccacctt
      421 caatttgctt acccaactca cgtatcagct ttgccgcatc gtaccccttt gattccacaa
      481 ggttatcgct gatcattaag gtcaggctag ccttgccctg aatatcggcc gccagcaggc
      541 aaacaagatc ggaaacctcg ttgcggagtt cgtaagcaag ttttttaata aggtctgcat
      601 taccgagatc gacctgagca cggatgatat tcatgccacc ggtagtttct atcgcattca
      661 ataaagaagc tttcacttgc tgaacttgca ataactgaaa ctgctctact tcttttttta
      721 gcgctgcatt ttcatcagcc agtgattgca cactttttac aacatcggaa actttggtga
      781 tatcttgtat ttttttgagc aattcatgct gctcatccat gtacttctcg atagcggaac
      841 ctgttatcgc ttcaatacgt cgtattccgg cagcaacagc actttcattt accaaacggc
      901 aatatccgat ttcgccggtt gcaccaacat gggtgccgcc acaaaattct ttgctataat
      961 tttcatcagc aataaccaca cgaacagtct cgccatattt ttcaccaaac aacatcatcg
     1021 cacccaattg ttgcgcttct gcaataggca tcacttttgt gacaacagga atattttcac
     1081 gaatcttggc atttacaatt tgctctatgc gacgcaattc ttcatcgctc actttagaaa
     1141 aatgcgaaaa gtcgaaacgc aaacgatcag gtcccacata ggaacctttt tgttgtacat
     1201 gtgaacctaa tacctgtcgc aaagcagcat gaattaaatg tgttgctgtg tgattataag
     1261 tgatatcagt tcggcgacgc gcatctactt cagcatacac aacagcatca gtattttcag
     1321 gcaactggtc ggtgaaatgg ataataagat cattctcttt cttggtatct aaaacacgaa
     1381 taatgttcgt attaatagat aaagttcctg tatcgccggt ttggcccccg ctttctgcat
     1441 aaaaaggtgt cttattcaga accacctgat attgcatttc attcttcatt ttcactgaac
     1501 gcattttcac cacgcgcgat tcagattgca gatgatcgta cccgataaaa tcaacagatt
     1561 tatcatcgcg cagcaccatc caatcgccgg tttccatgcc tgtagcttgc cgactgcgtt
     1621 ctttttgttt ttgcatttca gcttcaaatc cggcactgtc gacatccagt ccatgttcgc
     1681 gcacaatgag catggtgagg tctaatggaa aaccgtaggt atcaaataat tcaaaggcaa
     1741 ctgcgcccgg gattatttta gctgatttaa cttgttcaga gcgagttacg tcatccagtc
     1801 gttttatacc attttcaagc gtgcgtaaaa atgcgcgctc ttcttccata attacacgag
     1861 aaactaaatc gcgttgagca tccaattccg gaaatacatc gcggaattgt tctgcaagta
     1921 ccgcaaccaa ttcattgaag aatggttttt ttaaatgaag gaatgaatat ccataacgaa
     1981 tagccctgcg taaaattcga cgcaccacat aaccggcgcc attgttcgaa ggcagctgac
     2041 catcagcgat acaaaaactt acggcacgaa tatgatcact gataacacgc atggcaatat
     2101 ccgttttctc atcctgacca tattgaaaac cactaagtgt ttcaactttt ctgatcagcg
     2161 gtgtaaatac atccgtatca taatttgatg attttccttg caatgcgcgc accaatcgtt
     2221 cgaagcccat tccggtatct acatgtttat tcggcaattc ttccagactt ccatcagctt
     2281 tgcggttgaa ttgaataaaa acgtgattcc aaatttctat cacttgcgga tcgccggtat
     2341 ttacgagtgc agcaccatca atggcactgc gttcggcatc accacgcata tcataatgaa
     2401 tctcactgca tggtccacac gggccggtgt cacccatttc ccaaaaatta tcttttttat
     2461 taccacgcaa aatatgtgct tcactgatca tgcttttcca aacatcatat gcatccgtat
     2521 caaaagcaag attctctgac gggtcacctt caaaaacggt gacatataat ctggaaggat
     2581 cgagtttata aatatcggtg agtaattgcc atccccaggt aatactttct ttcttaaaat
     2641 aatcgccaaa actccagttt ccgagcattt cgaacatggt atggtgatag gtatctacac
     2701 caacttcctc aaggtcgttg tgcttgcctg aaacgcgcaa acatttttga gtatctgcaa
     2761 tacgcggata ttgcaccggt ttattcccaa ggaataggtc tttgaactga ttcatgcccg
     2821 cattagtgaa catcaaagta gggtcgttct tcaccacaat aggtgctgac gcaacgatct
     2881 tgtgttgctg gcgttcaaaa aattgcagga actgctgtct tatctctcgg gaggtcatca
     2941 tcgatatatc cggttttcgt tttatttttt ggccgggaag ctgtattttc gttgcccctc
     3001 taaagcacaa ttccccggcg agccgcaaaa ttacggaatt acacaggtaa ggcgcatggc
     3061 gaaaaaagta aaatatttct ataatccggc aaatctgcgc ttcgagaagc atgttacacc
     3121 tcggtgggtg tggaccttgc gcattattgg ctggatatca accgctgctg tttttgcctt
     3181 tatcatcgtt tggatagcct atacctattt cgattctcct aaagaacaat acctcaagaa
     3241 ccaaatctcc gaaatggagc tggagctcga tttcatgaac agtaagctgg ataccatggc
     3301 ggtggtattg ggtgaaatga ctgaacgaga cgataacatc tatcgcgtca tattcgaggc
     3361 ggaacccgtt tccagaagcg agcgtatggc aggtatcggc ggaagtgaac gctttcagcg
     3421 aatgaataaa ctcgacaatg ccgaactgtt aataaatacc gctacgaaac tcgaaagaat
     3481 acgccgacaa atggtgatac aatcgaaatc gtttgatgag atatcagaat tggtgagcaa
     3541 taaagagcaa cttctcagtc atacgccttc aatccagccg gtgagcaata aagatcttga
     3601 acgcattgct tcgggctttg gatggcgcat tcaccctatc tataaattcg caaaatttca
     3661 tgaagggctc gactttaccg ctcctaaagg ttcagatgtg tttgcaacgg ctgatgggac
     3721 tgtggtatta gctgataatt ctcaaagtgg ctttggaaac tgtatcgtaa tcgatcacgg
     3781 ttatggatat aaaacgcgat atgctcacct cagtgcattt aaagtgcgtg caggacaaaa
     3841 aatcaagcgc ggtgaactca taggactagt tggaaataca ggcctttcaa ctgcgccgca
     3901 cctccactac gaagtagaaa agaatggcac taaaatcgat cccatcaact tcttctataa
     3961 cgacctcagc gccgaagaat acgcccgaat gatcgaaatc gccaaccgca gtaatcagtc
     4021 ctacgattaa gggggtatcc ggtatcagac ttctctgcgg ctctgtgcct ctgcgtgaaa
     4081 aaatagcgat ctacctccct aagtacttta ccaatcaaat actatatatt tgttcttcaa
     4141 acccttatac acacccggca gtccttatca tgccctacaa agaaaaggaa atagaaaaag
     4201 tctactggtc gatcggcgag gttgctgaga tgcttgatct cagttttgcg cagattaggc
     4261 tctgggagaa ggaatttgat gtgttccaac tcaaaaaaaa taaaaagggc gatcgttact
     4321 tcacaaagaa ggatatagag caactcaagc taattaaata tctgctcaag gaaaaaaagt
     4381 ataccatcaa aggtgcacgt gagcagctga aatccaaaaa acctcaaacg gattcgcgat
     4441 tccaaaccat cgaagcgctc aagaagatca gacagttttt ggttgaactg aaagagcaga
     4501 tttgattagc caattagccg attagccaat tagccgatta gccgattagc cgattagctg
     4561 attagccgat ttgctgatta gccgattagc tgattagccg attagctgat tagccgatta
     4621 gctgattagc cgattagccg attagctgat tagctgatta gccgattagc tgattagccg
     4681 attagccaat tagccgatta gctgattagc cgattagctg atttggtgaa tagttacatg
     4741 gggtatgggc tccgaaggcg ctcaagttta catgtgtgca tgtgagcaag taagtaagtc
     4801 ccgtcgggag gtcggtgcgc tccatacaga agtatttagc tgattagcaa ataggcttgt
     4861 aagcatatgg agtttttcgc tttagtgagc aagtttgagg gttagcaagt tagcaggtgt
     4921 gctgctgaat gtaaatgccg aacctgaaca cacaaacaca tgaacacaat tggtgtctta
     4981 gcggtctctg cgtgaaaaac aaccaacggt agctactatt ccactaacag tagcgatgta
     5041 tatttgtaat acaaccggtg gtcaaaatag ctttgcgact tcaaaacaaa acatatgatg
     5101 ctacaagaaa gatcgatcgg aa
//
LOCUS       DAOQHY010000207         5107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_9332_length_5107_cov_5.132819, whole genome shotgun sequence.
ACCESSION   DAOQHY010000207 DAOQHY010000000
VERSION     DAOQHY010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9332_length_5107_cov_5.132819"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..550
                     /locus_tag="PLL28_13070"
     CDS             26..550
                     /locus_tag="PLL28_13070"
                     /inference="COORDINATES: protein motif:HMM:NF009807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HNF70304.1"
                     /translation="MTTTDLQYPVGKFQRPAEFNAARMPAFIDTIASFPEKLKATVLV
                     MSEAQLNAPYRPGGWSVRQVVHHCADSHMNSFIRFKLGLTEDNPTIKPYEEAAWAEMS
                     DYLKTDIAYSLMIIEGLHARWTSLLRNMSEADFQRTVFHPQQNREMSLFTLLALYAWH
                     CDHHLAHVQIVSRQ"
     gene            601..1050
                     /locus_tag="PLL28_13075"
     CDS             601..1050
                     /locus_tag="PLL28_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70305.1"
                     /translation="MKTLLWVSICICLITSCRYAYVKDYTGEYNFELYYLEYDSLGVA
                     YATDTLLCTGTISRYQEGDEANATTPIESYEIAADEKAWLTISYNNWTVICHVDESGA
                     IEPYSYGTSYTHSGEFTDDTHILLNTFWGGLGLYENIEVHGEKMLND"
     gene            complement(1138..3000)
                     /locus_tag="PLL28_13080"
     CDS             complement(1138..3000)
                     /locus_tag="PLL28_13080"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70306.1"
                     /translation="MKNPMSLIKKKSTCVALLAFAFAPAFAQSDIADPAFSSDGEYLS
                     MLRSDYLWDNDFVDEAGSITYWLNEPTGTGTTNYILRVLADGTPDLTLGLFGIKALES
                     ALIENPCDLPGEENCHTTTDITKAISLPGGKFLIGGMSYESDPIFSSWFVAQYLADGT
                     LDASFSDNGVIFGPIGKIHSVTGLGIDADGKILVLIEDYSSERSEIMRLNADGSFDDT
                     FGIDGLSILPDFTINCNDDIKTDASGNIYLAGNNSMPGDIVRLTSDGALDMSFGDEGR
                     VAFDTIDFHIEAFRIDDAGIVAAGEIELFVPSQFCLLRFDLDGNPDLSFSGDGHQFYI
                     PDSITPCPNYISSAAVISEANEFYISLNENAPDHYTSPIIFKFDADGNIDLSFSELGW
                     TWLSDAINRDIKLDADQRLIVSSEQYDALFGDHYIQIHRLLTDGTITGTEALLPCMID
                     EVCNGIDDNENGIVDEDLPMNVFYADLDGDSYGNPDAPAVTCALELSGYVMENTDCDD
                     TNALINSGAEELIDGLDNNCNGLVDEGTDVHNMQVQFAIYPNPTDGILYIETNNSTEG
                     LLTIVNATGQEVLRYKTPLTGPTIDLTSLPDGMYIVNVCYQNVCDAQLLVLSRN"
     gene            complement(3364..4749)
                     /locus_tag="PLL28_13085"
     CDS             complement(3364..4749)
                     /locus_tag="PLL28_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNF70307.1"
                     /translation="MRKFFQLLGQALKGENIDTTKGKLDTVIFLLAVPMIFEMGMESL
                     FAVVDIFFVSKVGVEAVAVVGLTESTLTIVYSIGFGLSMGATAMVARRVGENNPKGAA
                     EAAMQAIYIGLAIAAAVSLVGIFFPENLLRAMGASDQMIQNGVGYTRWMLTGNFAIVL
                     LFLNNGIFRGAGDAGIAMRSLIISNGINIVLDPLFIFGIWIFPELGVQGAAVATTIGR
                     SIGVIYQFYHLTNGKSLVHLKGKIWQLKTDIVRRIVILSSGSAGQFIIASASWIFLMR
                     IMSQFGEETLAGYTTAIRILVFSILPAWGISNAAATLMGQNLGANQPERAEKGVWRTA
                     YFNMLFMAVVTVIFVGFPEFFMRIFNSDDAVVNQGVTCLRIVCIGYIAYGYEMVLANA
                     INGAGDTRTPTIINVFGFWILQIPLAWFMAIQLDLGSPGVYAAVPISEAVMAVIYVYI
                     FRQGKWKLVKI"
BASE COUNT         1623 a         1112 c          953 g         1419 t
ORIGIN      
        1 gccatttcaa taaaccgcga aagatatgac cactactgat ctgcaatacc ctgttggcaa
       61 atttcaacgt cccgctgagt tcaatgctgc ccgtatgcct gcatttattg ataccattgc
      121 aagctttcct gaaaaactga aggcaactgt tttagttatg tctgaagcgc aactcaatgc
      181 accttacaga cccggtggtt ggtcagttcg acaagtggta catcattgtg cggatagcca
      241 catgaatagt tttattcgat tcaaactggg actcacagag gacaatccaa caataaaacc
      301 ttacgaggaa gcagcttggg ctgaaatgtc ggattatctc aaaacagata tcgcatattc
      361 cctgatgatc attgaaggat tgcatgccag atggacctcg ctattgcgca atatgtcaga
      421 agctgatttt caacgcacgg tatttcatcc acaacaaaac agagagatgt cattattcac
      481 cttactcgca ctttatgcct ggcattgtga tcatcatttg gcgcatgtgc agattgtaag
      541 tcggcaataa tgaatatcta attacaaatc accttacttc ctcacataaa atgctcgaat
      601 atgaaaacat tgctttgggt atccatttgt atctgtttga ttacatcctg ccgctacgcg
      661 tatgtcaaag attataccgg tgagtacaat tttgaactat actatttgga atatgacagt
      721 ttaggcgtag cctatgccac agacacactg ctatgcaccg gcaccatatc gcgatatcaa
      781 gagggcgatg aggcaaatgc cacaacccca atagaatcat acgagatcgc tgccgacgaa
      841 aaggcttggc tcactatttc atataacaat tggacagtga tatgccatgt agatgaatca
      901 ggtgcaattg aaccgtattc ctatggaaca agctatacgc attcaggaga attcactgac
      961 gatacacata ttctgctaaa tactttctgg ggtggccttg ggttatatga aaatattgaa
     1021 gtgcatggtg aaaaaatgct caatgattaa ttggcagatc gagatacact aaaataatag
     1081 acaaaaaaaa tacaccggac ttacattaaa cctggtgtat tttttttaat acatttatta
     1141 atttctgcta agcactaaca attgcgcatc gcaaacattc tgatagcaaa catttacaat
     1201 atacataccg tcaggaaggg aggtcagatc aatagtggga ccggtaagtg gggttttata
     1261 tctaagcact tcctggcctg ttgcatttac aatagtcagc aatccctctg ttgaattgtt
     1321 tgtttcaata tataatattc catctgtggg attaggataa atcgcgaatt gcacttgcat
     1381 attatgcaca tcagtgccct catctacgag gccattgcaa ttattatcga gaccatcaat
     1441 taattcctca gcaccactgt taatcagggc atttgtatca tcgcaatcag tattctccat
     1501 cacgtagccg gacagctcca atgcgcatgt aaccgccggt gcatcagggt ttccgtaaga
     1561 gtctccatct aagtcggcat agaagacatt cataggaaga tcttcatcca caatcccatt
     1621 ttcattatca tctatcccgt tgcaaacttc atcaatcata catggcaaca gtgcttcagt
     1681 gccggtgata gttccatctg tcagcaaacg atgtatctga atatagtgat cgccaaataa
     1741 tgcatcatat tgctctgaac taacaatcaa tcgttgatca gcatcaagct taatatctcg
     1801 gttaattgca tccgacaacc atgtccatcc caattctgaa aacgacagat ctatatttcc
     1861 atccgcatca aatttgaaaa taattggtga agtataatgg tcgggagcat tttcattcag
     1921 actgatataa aattcatttg cttctgaaat cacggcagct gacgaaatgt aatttggaca
     1981 aggtgttatt gaatccggaa tataaaattg atgcccgtcg cccgaaaatg acaaatcagg
     2041 attgccgtca agatcaaatc gaagcagaca gaactgcgat ggaacgaata attctatttc
     2101 cccggcagct acaattcctg catcatcaat tcggaaagct tctatatgaa aatcaatggt
     2161 atcgaacgcg accctgccct catcaccaaa actcatatcc agtgcaccat ctgaggttag
     2221 tcttacaata tcgccgggca tactattatt tcccgcaaga tagatattac ccgatgcatc
     2281 agtttttata tcatcattgc agttgatcgt aaaatccggc aatatagaaa gcccatctat
     2341 accaaaagta tcatcgaagg aaccatctgc attcaaacgc atgatttcag atcgctctga
     2401 tgaataatct tcaatcaaaa caagaatttt tccatccgca tctattccaa gtccggttac
     2461 actgtgaatt tttccgattg gaccaaatat tacaccgtta tcgctaaaac ttgcatcaag
     2521 tgttccgtca gccaggtatt gtgctacaaa ccatgaagag aaaattggat cagattcata
     2581 cgacatacct ccaatcagga acttacctcc gggaagagaa atagcctttg tgatatctgt
     2641 agtagtatga caattttcct cgccgggaag gtcgcaggga ttttcaatta atgcactttc
     2701 caatgcctta atcccaaaca aacccaaggt aagatcaggc gtgccatctg caagtacgcg
     2761 caaaatgtaa ttcgtagttc ctgttcctgt aggttcattc agccaatagg taatgctgcc
     2821 tgcttcatcg acaaaatcat tatcccacaa gtagtcggat cgcaacatgg aaaggtattc
     2881 accatccgag gaaaatgccg gatcagcaat atccgattga gcgaatgcag gtgcgaaggc
     2941 aaaagcaagc aatgctacgc aggttgattt ttttttaatg agtgacatgg gatttttcat
     3001 gcgcagatat tttggggttt atattgaatt aatgctaata tgaaataaaa ttcaaattat
     3061 cattatttac taaactacgg gtttaaaccc gtttttcata ggagaatcgg aaaaaaattg
     3121 gcattttttc gttttcatct cccacaaagc caaaattcaa cggtaattac tagaactcat
     3181 ctcaaaaata ggtttgattt agcattttca agtattcgcg ctttagccga ggcgcttgaa
     3241 gtcgccccta ctggaggttg taagggcaat tcaagtaacg atggataaag agcgaagacg
     3301 cagaaaagac gccaaaggta tttttgagat gagttctaca tacaatcaca aaaagataat
     3361 gaattaaatt tttaccaatt tccactttcc ttgtctgaag atatagacat aaataacagc
     3421 catcactgct tcagaaatag gcacagcggc atatacccct ggcgagccaa ggtcaagctg
     3481 aatagccata aaccaggcta atggtatttg caatatccag aatccaaata cattgatgat
     3541 ggtaggtgtt cgagtatcac ctgcaccgtt tatggcattg gccagaacca tttcatatcc
     3601 ataagcgata tatccgatac aaacaatccg caagcatgta acgccctgat ttacaacggc
     3661 atcatctgaa ttaaatatgc gcatgaaaaa ctcaggaaac ccgacaaaaa tcactgtcac
     3721 cactgccata aacaacatat taaaatatgc tgttcgccat acaccctttt cggctcgttc
     3781 tggttgattt gcaccaagat tttgccccat taacgttgca gcggcattcg aaattcccca
     3841 agccggcaaa attgaaaaaa ctaaaatgcg tattgccgtg gtataacctg ctaatgtttc
     3901 ctcgccaaat tgcgacatga tgcgcattaa aaaaatccaa ctagccgaag caataataaa
     3961 ttgaccggca ctacctgaag atagtatgac tatccttctc acaatatctg tttttaattg
     4021 ccagattttc cctttcagat gcaccagtga ttttccattt gtgaggtgat agaactgata
     4081 tattacacca atacttctac ctattgttgt tgccactgcc gcgccttgca ctcccaattc
     4141 aggaaatatc catattccaa aaatgaacaa aggatccagt acaatattga ttccatttga
     4201 aatgatcaaa ctgcgcattg cgatgccggc atcacctgct ccacgaaaaa ttccattatt
     4261 taaaaacaat aaaacaatgg caaaatttcc ggtaagcatc catcgagtat aaccgactcc
     4321 attttgtatc atctgatcac ttgcacccat ggcgcgcaac agattttcag ggaaaaatat
     4381 accaaccaat gatactgcag ctgcaatggc caatcctatg taaatagcct gcatggcggc
     4441 ttctgcggct ccttttggat tattttcacc tactctcctc gccaccatcg ccgttgcgcc
     4501 catactcaat ccgaacccaa ttgaatacac aatggtaagt gtactttctg tcagtccaac
     4561 aacggcaact gcttctacac caactttgct gacaaagaaa atatcgacta ccgcaaacaa
     4621 actctccata cccatttcaa atatcatggg tactgcaaga agaaaaatta cagtatctaa
     4681 tttcccttta gtagtatcta tattttcgcc ctttagggcc tgacctaata attgaaaaaa
     4741 tttacgcatg cctatgcctt tcaaaataat aaattataag aaattacctt ttccatgcag
     4801 ctcatccacc acagtgcaga aagaaaaata aattgtggat gctaaacaat gtgttaaagt
     4861 ggaagcatcc taattaaagg atcatatgca ataattgatt gcgcaaagat aattgaattg
     4921 gaggagaaag gagaaaggag agaggagaga ggagagagtg agtgggtgag tgggtgagtg
     4981 ggtgagtggt gaatcgtgag tcgtgagtgt ggtgcgtact tgagaatcgg gtatacgatg
     5041 cgaacatgca tttgatacct gatacttgat acttgatacc tgatacctga tacttgatac
     5101 ctgatac
//
LOCUS       DAOQHY010000208         5080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_9399_length_5080_cov_6.028856, whole genome shotgun sequence.
ACCESSION   DAOQHY010000208 DAOQHY010000000
VERSION     DAOQHY010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9399_length_5080_cov_6.028856"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PLL28_13090"
     CDS             <1..690
                     /locus_tag="PLL28_13090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNF70308.1"
                     /translation="TSDFDVDASLCYGSTIQDDGKIIAAGYTYDINGDESIAIARYAS
                     CSAYFTLFPDATPHVWKAVNMAVGSDPMSYVWEWGDGTSSTEPYPSHTYADSGYYDIC
                     LTITDGLGCTSTYCDSSTYIYRMSDELAMVTINVWEELPVETETITADFSVTPNPFSD
                     QITIEIPSAVSSSPNAEISIFDLAGNILLTQIISAPQTTINTSQLPTGIYVLSYHNGL
                     EFGTAKLVKME"
     gene            1109..1459
                     /locus_tag="PLL28_13095"
     CDS             1109..1459
                     /locus_tag="PLL28_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70309.1"
                     /translation="MSLFDNIKKEEVEIIATVKKIEIVYTYKGDIQKITLICDSEKLK
                     KGYETYFNYSTKWSKFNLKSGDKLKFKAILKTTTVTVHKDERWVDDWGTLPIHLTDMV
                     DFIEIQSPKQIMLF"
     gene            complement(1603..>3141)
                     /locus_tag="PLL28_13100"
     CDS             complement(1603..>3141)
                     /locus_tag="PLL28_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70310.1"
                     /translation="TFDSQSQNLFFASPDGMSGTPSFRSILATDLPIVGSGTQGIISN
                     NTGSTQVFGDGDKEFPDKLFLSNGTTANPSLAFSGAHTTGLSWVTGEAINGSFSGTQR
                     FQFTSLGKLVLSDCEEFDPKLDFNIYHDSNDDSPAQDGGINITTYGGTDCVYPDCHFA
                     TVTGRTASGSKGTPQATVVDQGLIELSGKGYTGSAFETSNRGVFGIYAAETHDGSGQG
                     TYALVRTTPINSTTIHTSMLIDPEGNAGLTHSSFNTSLPNGWDATADSRFFSIESNAT
                     NKDAGILMQNASGNRGLNVWFDYSGNFAYIDDIRNDQASGVGFRFRTAGTPEAALTML
                     YNDAGDHVYINAGKDVTPDNLFTVGDNSEFQVDVNGKVAKYNNIATVSNGVPAEYAIA
                     QATNKSAAQAATTIYTTPAADGFYRISWVCTITTQASTSSILGPFTVSYTGATDNVVK
                     TWPSNNINNFNQCATNSTATGVISGCMTIYAKASTNIQFTVGYTSSGVTSMVYDYNIV
                     CEKL"
     assembly_gap    3142..3241
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3242..3440)
                     /locus_tag="PLL28_13105"
     CDS             complement(<3242..3440)
                     /locus_tag="PLL28_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70311.1"
                     /translation="MKPVSILFILICSCIYLQAQVTSVGLAAPTAVFDVSGSPVTTTG
                     TLTLTFDSQSQNLFFASPDGMS"
     gene            complement(3470..>5080)
                     /locus_tag="PLL28_13110"
     CDS             complement(3470..>5080)
                     /locus_tag="PLL28_13110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HNF70312.1"
                     /translation="VVKLDSVGNILWQNTIGGSSSDQLTNIIQTRDDGFLIGGRSSSN
                     ISGDKTENAIGGTDYWVVKLDSVGNILWQNTIGGLNFEELYELMQTEKGDYLIGGNSN
                     SGISGDKTEASILNPFGLNTHDYWIVEIDSIGNIIWQNTIGGNDADYNYTLDLISNDG
                     LITGGSSKSNISADKTSESFGDLDYWIVALDTLGNVEWQNDFGGNNSDYLKGIIQTFD
                     KGFALVGYSLSGSTGNKVEGNYGMEDYWIIKVDTLGNISWQNSLQGTGSDFACCIIQA
                     PDGGFLVGGTSDSNISPDKNEDNIGNGDYWIVKFYPEDCTLYTFYKDFDEDGYGSMGD
                     SVVACYIPDGFVENNFDCNDFNPMIHPDQGDSCNDIDDNCNGILDEDATFTTYFLDYD
                     EDGYGNPLIDTISCSTIMGFVLNDDDCDDSNPLVYPGAIELENGVDDNCNGSVDEGFN
                     YIENIDTSIYKIFPNPNTGEFKIYVNSSIIELLVIKIYSITGELLYYSTFLSSNEIKM
                     SLPEAFSGLYLLSVVSDKVYIFPIEIIK"
BASE COUNT         1607 a          977 c          817 g         1579 t
ORIGIN      
        1 acttccgact ttgatgttga tgcaagtcta tgttatggtt caactattca ggatgatgga
       61 aaaataattg ccgcaggata tacctatgat ataaatggtg atgaatcaat tgcaattgct
      121 cgatatgcct cttgctccgc ttatttcact ttattccctg atgcaacccc gcacgtatgg
      181 aaggccgtaa acatggctgt cggctctgat ccgatgagct atgtgtggga gtggggtgat
      241 ggcacctctt ctacggaacc atatccctca catacctatg ccgattccgg ttactacgac
      301 atttgcctaa ccataacaga cggacttggc tgcacatcaa cctattgcga ttcatcaact
      361 tacatttaca gaatgtctga tgaacttgct atggtaacta ttaatgtatg ggaagaatta
      421 ccggtagaaa cagaaacgat aactgctgat ttttcagtca cacccaatcc attttccgat
      481 caaattacaa ttgagattcc gtcggctgtg tcaagcagtc cgaatgcaga aatttcaata
      541 tttgatttgg ctggaaatat actattaacg caaatcatca gcgctcctca aacaactatc
      601 aatacatctc agttgccaac cggtatttat gtgcttagtt atcataatgg actggagttt
      661 ggaacggcca agttggtgaa aatggaatga taacttgaaa agtgcaggtg agcgtttcag
      721 actgctgtta aaaggtaaat tgaggtaata ttattgtggt tgagtgtggt catgttgttt
      781 tccttactaa tgtttttaaa atattgtcag aattgatagg ccctgtttgt attcaatcaa
      841 agtaatctct tcaccgatgg cattaccaat atgaatttca ttgttgatgt tgttttttaa
      901 ggcataaaca aatactgaca tatcattgat ttaagggtaa aaaaaagacc gaccagcatg
      961 ctgctggtcg gtcttaaata attgttttcg atgtagccca aaagatacca ttatcgaacc
     1021 aaatagtgtt gagaatgaag gagttaaggg atttcaaatg caattttttc gctaatatta
     1081 caacgccttt acaaatcatt aaccaacaat gagcttattc gacaatataa aaaaggaaga
     1141 ggttgaaatt atcgcaactg tcaagaaaat agaaatcgtc tacacttaca aaggggacat
     1201 ccagaaaata acgctcatct gtgattcaga aaagcttaaa aagggttacg aaacatattt
     1261 caattactca actaaatggt cgaaatttaa ccttaagtct ggtgataagc taaaatttaa
     1321 ggcaattctc aaaaccacca cagtaacggt tcataaagac gaaaggtggg tggatgactg
     1381 gggcacattg ccaattcact taaccgatat ggtagatttt attgaaatac aaagccctaa
     1441 acaaattatg ttgttttaac cactcttaat tttttaacgt tccaatttta aataaatgtc
     1501 taaaatttaa atcccaaatt ctatatattt caatggatgc tatttattat tcaaaacctg
     1561 caatgaattt tattctatat agcaaaatta aggtggattc atttatagtt tctcacaaac
     1621 aatattatag tcgtaaacca ttgatgttac accagaactt gtataaccaa cagtaaattg
     1681 gatatttgta ctagctttag catagatagt catacatccg ctaatgactc ctgtagctgt
     1741 actattagtt gcgcactgat taaaattatt tatattgttt gaaggccatg tttttaccac
     1801 attatcagta gcacctgtgt aggaaaccgt gaatggtcct aaaattgatg aggttgaggc
     1861 ctgagtggtg atagtgcaca cccaacttat tctataaaat ccatcagcag caggcgttgt
     1921 atatattgtt gttgcagctt gcgctgcaga tttatttgtc gcttgagcaa ttgcatattc
     1981 tgcagggact ccattgctca ccgtagcaat attattatat tttgcgactt tcccatttac
     2041 atctacttga aattcactat tatctcccac ggtgaataga ttgtcaggag tcacgtcttt
     2101 acctgcattt atgtatacat ggtcgccagc atcattatat agcatagtta aagcagcttc
     2161 tggcgttcca gcagtcctaa atcgaaatcc tactcccgaa gcttgatcat ttctgatatc
     2221 gtcgatatat gcaaaatttc cagaataatc gaaccataca ttaagacctc tatttcctga
     2281 agcgttctgc attagtattc cggcatcttt atttgttgca tttgattcaa ttgaaaaaaa
     2341 cctactatct gcagttgcat cccaaccatt tggcaatgag gtattgaatg aactatgagt
     2401 taatccggca tttccctcag gatcaattag catacttgta tgaattgtgg tcgaattaat
     2461 tggtgtagtc cgcactagag cataagttcc ttgtccagat ccatcatgtg tttcagctgc
     2521 ataaattcca aaaacaccac gatttgatgt ttcaaatgca cttccagtat aaccttttcc
     2581 tgaaagctca attaatcctt gatctacaac agttgcctga ggggtgcctt tactaccaga
     2641 ggctgttcta ccagttacag tagcaaaatg acaatcggga taaacgcaat ctgttccacc
     2701 ataggtagta atattaattc cgccatcttg tgcggggctg tcatcattgg agtcatggta
     2761 tatgttgaaa tccaatttgg ggtcaaattc ttcacaatca gatagcacta atttacccag
     2821 actggtaaat tgaaaccttt gagttccgga aaaagaacca ttaatagctt cacctgtaac
     2881 ccaactcagt ccagttgtat gtgccccgga aaatgctaat gaaggattag ctgtagttcc
     2941 atttgataaa aatagtttat caggaaattc cttatctcca tcgccgaata cctgggtaga
     3001 tcctgtatta ttgctaataa taccttgcgt tcctgacccc actattggta aatctgttgc
     3061 caatattgat ctgaaactgg gggttcccga catcccatcc ggtgaagcga aaaataaatt
     3121 ttgagattga ctgtcaaagg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nccgacatcc catccggtga agcgaaaaat aaattttgag attgactgtc aaaggtcaat
     3301 gtgagagtac ccgttgttgt cacaggtgaa cctgaaacat caaacactgc agtaggagca
     3361 gcaagtccta cagaggtaac ttgagcttgc aaataaatgc aactacaaat tagaataaat
     3421 aaaattgaaa cgggtttcat attacttaat tttgggttaa aaaataaatt tatttaataa
     3481 tttcaatagg gaatatataa accttatctg atactactga taacaaatac aatcctgaaa
     3541 atgcctcagg taaagacatt tttatttcat ttgaagaaag aaaagtagaa taataaagca
     3601 attctccagt tattgagtaa attttaatta ctaaaagttc aataattgag gaattaacat
     3661 agattttgaa ttctccagta tttggattag gaaatatttt ataaattgag gtatcaatat
     3721 tttctatata attaaatcct tcatcaactg aaccattaca gttatcgtct acgccgtttt
     3781 ctaattcaat tgcacctgga tatacaagag ggtttgaatc atcacaatca tcatcattta
     3841 aaacaaatcc cataattgta gaacaagata tggtgtcgat taatggatta ccataaccat
     3901 cctcatcgta atccaaaaag taagtagtga atgttgcgtc ttcatcaaga ataccattac
     3961 aattgtcatc aatgtcatta caagagtcgc cttgatctgg gtgtatcatg ggattaaaat
     4021 cattacaatc aaaattattt tcaacaaacc catcaggaat gtaacatgca actaccgaat
     4081 ctcccataga accataacca tcttcatcaa aatctttata gaaagtgtat agtgtacaat
     4141 cctcaggata aaatttaact atccaataat caccattccc aatgttatcc tcatttttat
     4201 caggcgaaat atttgaatcg gaggttcccc caactaaaaa tccaccatca ggtgcttgaa
     4261 taatacaaca tgcaaaatca ctaccagtgc cttgtaggga attctgccat gaaatattgc
     4321 caagtgtatc tactttaatt atccaataat cttccattcc ataattaccc tcaaccttat
     4381 tgcccgtcga gcctgataat gaatagccga cgagcgcaaa ccctttgtca aatgtttgta
     4441 taataccttt taaataatct gaattatttc caccaaaatc gttctgccat tcaacatttc
     4501 ctaaggtgtc taatgcaact atccaataat ccaaatcacc aaagctttcc gaagtcttat
     4561 ctgctgaaat atttgatttt gagcttccac ctgtaattaa tccatcatta gaaattagat
     4621 ccaatgtata attgtaatca gcatcatttc ctccaatggt gttttgccaa attatatttc
     4681 ctatagaatc aatttcaact atccaataat catgtgtgtt caaaccgaac ggattgagaa
     4741 tacttgcttc cgttttatca cctgaaatac cagaatttga gttcccacct attaaataat
     4801 cacctttttc ggtttgcatt aattcatata attcttcaaa atttaatcct ccaatcgtat
     4861 tttgccacaa tatatttcca acactgtcaa gcttaaccac ccaataatct gtccctccaa
     4921 ttgcattttc tgttttgtct cctgagatat ttgaacttga cctcccccca atcagaaatc
     4981 catcatcacg agtttgaatt atattagtta attgatcact tgaagatcct ccaatcgtat
     5041 tttgccacaa tatatttcca acactgtcaa gcttaaccac
//
LOCUS       DAOQHY010000209         5057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_9474_length_5057_cov_5.828669, whole genome shotgun sequence.
ACCESSION   DAOQHY010000209 DAOQHY010000000
VERSION     DAOQHY010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9474_length_5057_cov_5.828669"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1448)
                     /gene="mnmE"
                     /locus_tag="PLL28_13115"
     CDS             complement(<1..1448)
                     /gene="mnmE"
                     /locus_tag="PLL28_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNF70313.1"
                     /translation="MSPIVLLNPFTSDDGIFLKLLCTFAGMFVADHKDTIVALSTAPG
                     VGAIAVIRMSGDKAIDIMTAVFKGKNLAEAKANTLHFGRIESETTIIDEVVVGIFRAP
                     RSYTGEDIIEVSCHGSPYIQQQILTLLIAKGARMAKPGEFTMRAFLNGKMDLTQAEAV
                     ADLIAAQSQASHDVAIKQMRSGYGDALKNLRYELIHFASLIELELDFSEEDVEFAERS
                     HLMHLLEQINVELDRLIKSFSLGNVIRNGVETVIAGRPNAGKSTLLNALLNEDRAIVS
                     EIAGTTRDTIEAELNIKGVRFRLIDTAGIREAKDEIEKIGVAKTMEKIKTATLLIYVF
                     DVHTMTPEEVGADLDSLVSSGDNMLVIANKIDAAGGQERAALFTKVNPDGFISSLQKV
                     NIERLKDAMFARIVHEDITLEDAVVTNARHLEALRKARATMTEVQDAIAQKTSGELTA
                     LSIRYALNAIGEITGEVTNDDLLDNIFRKFCIG"
     gene            1402..2160
                     /locus_tag="PLL28_13120"
     CDS             1402..2160
                     /locus_tag="PLL28_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70314.1"
                     /translation="MPSSDVNGFNKTMGLIVAFIGLPVCFIGLTGYIAIGRHGMGWIF
                     LILALSGITIIAGGVQMFFQGRADAAKQREVLQAAKAGKAESQITKIITEWPIDDKTW
                     ADFCINEKHYRRGDNYIFFFMFLAIGSFILVASRGSGLLLAVCISGVIGLITVILRYN
                     VAMKKVHTISGIEKKVIIGKRYIIMNGILYPINNERMTTRKIQLITTSIPAILEFTIY
                     WPTRSGETFDELRIPVPDEAISNAKLVVNSFTSN"
     gene            2181..2975
                     /locus_tag="PLL28_13125"
     CDS             2181..2975
                     /locus_tag="PLL28_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70315.1"
                     /translation="MKYITLACMLCLSGLNVFAQFKPGDIGYNVNSEIIFKNAHVSSQ
                     IATVVESEDMSFVVNYNYYNSNGQLDSTVYPPDYNVDQDEQFPQMKDIYTYLPDGKIS
                     MITMKGYDMFDINYGFEYNKKGKLITSLVASAEAREYTYTYDNKGRISERNGKGARFV
                     YENDEDTDGKLVMIDIERSTYTWNPNGDLSEVNFYYMNEWMQRTVYSYNEDHQVTRMD
                     VYNDNGPDANPSFYISYQYNNQGLLTESTTVESDYTGKTTYAYTYY"
     gene            3010..3471
                     /locus_tag="PLL28_13130"
     CDS             3010..3471
                     /locus_tag="PLL28_13130"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HNF70316.1"
                     /translation="MKVLMVCLGNICRSPLAEGILRHKSQEKGLNWHIESAGTGAWHV
                     GQAPDPRSQRVAKQHGIDISKQRAQQFSPYHIEMFDLIFAMDSSNYQDIIRHAKTDAE
                     KNKIQLIMNMVEPGMNKAVPDPYYDDHLYATVYQMLDEACDNIISKYAINN"
     gene            3513..4913
                     /gene="hisS"
                     /locus_tag="PLL28_13135"
     CDS             3513..4913
                     /gene="hisS"
                     /locus_tag="PLL28_13135"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNF70317.1"
                     /translation="MSKQKPSIPKGTRDFGPDEVAKRNYIIGTIRKVYELYGYQPLET
                     PVMENLSTLMGKYGEEGDKLLFKILNSGDYLKDADTQLLSEKNSNKVLSAISEKGLRY
                     DLTVPFARYVVMNQNEISFPFKRYQIQPVWRADRPQKGRYREFYQCDADVIGSNSLLL
                     DAEMVMMIADVFSKLGLRVTIRLNNRKILDGLNEYIGETEKNVALITAIDKLDKIGKE
                     GVQNELREKGFTDDQISKTENILAITGSATDKISALEKLFSGEKGKKGISEIKELFAK
                     IANVGSSNIQIAFDLTLARGLSYYTGTIYEVVSNDVQMGSILGGGRYDDLTGIFGLPN
                     MSGVGISFGLDRIYDVITELNKFPESATLQTQVLFIAFDESAETAALQYARSLRDAEI
                     RCELYPDVQAKTAKKFKYADDKKIPYCAIIGSNEMAEQKVMLKNMQSGDQQLLSIEEV
                     IEIITGIRSNFVDLSE"
BASE COUNT         1601 a         1045 c         1007 g         1404 t
ORIGIN      
        1 ccaatacaaa acttcctaaa aatattatcc agcaaatcat catttgtcac ctcacccgta
       61 atctcgccga tggcgttgag ggcgtagcgg atggagaggg ccgtgagttc gccgctggtt
      121 ttttgggcta tggcgtcttg aacttcggtc atggtggcgc gggctttgcg aagggcttcg
      181 aggtgacggg cgttggtgac tacagcgtct tctaatgtga tatcctcatg cacaatgcgc
      241 gcaaacatgg catcttttaa tcgctcgata tttacttttt gtagcgaaga aataaatccg
      301 tcgggattca ccttcgtaaa taatgcagcc ctctcctgcc ctcctgccgc atcaatttta
      361 ttggcaatta caagcatgtt atcaccactg ctgacaagac tatctaaatc agcacccact
      421 tcctcaggtg tcatcgtatg cacgtcaaaa acataaatca acagtgtggc tgtttttatt
      481 ttctccatcg ttttggcaac accaatcttc tctatttcat ccttcgcctc acgaattccg
      541 gcagtatcga tcaatcgaaa gcgaacacct ttaatattta attctgcttc aattgtatcg
      601 cgagtagttc ctgcaatttc gcttacaatg gcacgatctt cgtttaataa tgcattcaat
      661 aaggtcgact tacccgcatt cggcctgcct gcaataacag tttcaacacc attgcgaata
      721 acatttccta aagaaaaaga tttaattaac ctgtcgagct ctacatttat ctgttcaagc
      781 agatgcatga ggtgacttct ctcggcaaat tcaacatctt cttcgctaaa atctaattcc
      841 aattcaatca aggatgcaaa atgaattaat tcatatcgca gattttttaa tgcatcacca
      901 taaccggatc gcatttgttt tatcgccaca tcatgtgatg cctgcgattg cgccgcgatc
      961 aaatcggcaa ctgcttctgc ctgcgtaaga tccatcttac cattcagaaa tgcgcgcatc
     1021 gtaaactctc caggcttcgc catacgcgca cctttggcaa ttaacaacgt aagtatttgt
     1081 tgttggatat acggagaacc gtgacaactc acttctataa tatcctcacc cgtgtaagat
     1141 ctgggtgctc tgaaaatacc gacaaccacc tcatctataa tcgtagtttc agactcaatc
     1201 cttccaaaat gcagggtgtt agcctttgct tcagcaaggt ttttcccctt gaatactgca
     1261 gtcataatgt ctattgcctt gtcgcccgac atgcgtatca cagcaatagc accaacaccg
     1321 ggggcagtac tcagcgcaac tatagtatct ttatgatcag caacaaacat acctgcgaag
     1381 gtacacagaa gctttaagaa tatgccatca tctgatgtaa acggatttaa taaaacaatg
     1441 gggctcattg tagcctttat aggccttcct gtatgtttta tcggacttac aggctatatc
     1501 gccattggcc gtcatggaat gggttggata tttctaatct tggctttaag cggcatcaca
     1561 atcattgcag gtggcgttca aatgttcttt cagggcagag ccgatgcagc aaaacaaaga
     1621 gaagtgcttc aagcagctaa ggcaggtaag gcagaatctc aaattacaaa gattataacc
     1681 gagtggccta ttgatgataa aacctgggca gatttctgca taaacgaaaa acattatcgt
     1741 cgcggcgata attatatttt cttctttatg tttcttgcca taggcagctt cattttagtt
     1801 gcatccagag gttcaggttt actgttagcg gtatgcatca gcggagtaat tggattgatc
     1861 acagtgattt tacgttacaa tgtcgccatg aagaaagtgc atactataag tggcatagaa
     1921 aaaaaagtga ttattggtaa gcgatacatc attatgaatg gcatccttta tcctatcaat
     1981 aacgagcgga tgacaacacg caaaattcaa ttgatcacaa caagcatacc ggcaattctg
     2041 gaatttacga tttactggcc aacacgaagc ggagaaacgt ttgatgaatt gcgcatacct
     2101 gtgcctgacg aggctattag taatgccaaa ttggtagtaa attcttttac atccaactaa
     2161 aacaaagaaa atatatcact atgaaatata taaccttagc ttgcatgcta tgtttatcag
     2221 gcctgaatgt gtttgctcaa tttaaacccg gcgacattgg ctataatgta aacagcgaaa
     2281 taatattcaa aaatgcacac gtttcatctc aaatagccac tgtggttgaa tctgaagata
     2341 tgtcatttgt ggtaaattat aattactata acagtaatgg gcaattagat tcaacggttt
     2401 atcctccgga ttataatgta gatcaggatg aacagttccc ccaaatgaaa gacatatata
     2461 catatttacc agatggcaaa atcagcatga ttaccatgaa aggttacgac atgtttgata
     2521 taaattatgg atttgagtat aataaaaaag gcaaacttat aacaagcctg gtcgcaagtg
     2581 ctgaagcccg ggagtacact tatacctatg ataacaaagg aagaatatca gaaagaaacg
     2641 gcaaaggagc aaggtttgtt tatgaaaacg acgaagatac tgatgggaag ttggtgatga
     2701 tagatattga acgatctact tacacatgga atccaaacgg cgacctgtca gaagtaaact
     2761 tttattatat gaacgaatgg atgcaacgca cagtttactc ctacaatgaa gatcatcaag
     2821 tgacaagaat ggatgtatat aacgataatg ggccggatgc taatccttca ttttatatca
     2881 gctaccaata taataatcaa ggtttgctga ctgaatcgac tacagttgaa tctgattata
     2941 ccggaaaaac cacatacgca tatacgtatt attgatttac atgtgatcaa tagtctgtaa
     3001 ttttgcagca tgaaagtgct gatggtttgt ttgggtaata tttgtcgcag tccccttgca
     3061 gaaggtatat tgcgtcataa atcgcaggag aaggggttga actggcatat cgaaagcgcc
     3121 ggcaccggag cctggcatgt aggacaagct cctgacccac gatcacagcg tgttgccaag
     3181 cagcacggga ttgacatcag caagcaacgt gctcagcaat tcagtccgta tcatatagaa
     3241 atgtttgatt taatatttgc aatggatagc agcaattatc aggatatcat cagacatgca
     3301 aaaacggatg cagaaaaaaa taaaatccaa ctgatcatga atatggtgga acccggcatg
     3361 aacaaagcag ttcccgatcc atactacgat gatcatctgt acgcaacagt atatcaaatg
     3421 cttgatgaag catgcgataa tatcatttcg aaatacgcaa taaacaatta ataattttac
     3481 cacagcaatc aggctgatat aatataattt tcatgagcaa gcaaaaacca agtataccaa
     3541 aaggaaccag agatttcgga ccggatgaag tggctaaacg caattatatc atcggcacca
     3601 taagaaaggt ttatgaatta tatggatatc agcctttaga gacacctgtc atggaaaatc
     3661 tctcaacgct catgggtaaa tatggtgaag agggtgataa attattattc aaaatactca
     3721 acagcggcga ttatttaaaa gacgccgaca cacaattact gtcagaaaaa aattcaaaca
     3781 aagtcctcag tgctatttca gaaaaaggtt tacgttacga tctcacagtg ccctttgcgc
     3841 gatacgtggt gatgaatcaa aatgaaatca gcttcccatt taaacgctat caaatacaac
     3901 cggtatggcg cgctgatcgt ccgcagaaag gccgctaccg cgaattctat cagtgtgatg
     3961 ctgatgtgat cggcagcaat agtctattgt tggatgcaga aatggtgatg atgatcgccg
     4021 atgtgttttc gaaactggga ttgcgtgtta ccattcgact caacaatcga aaaatattag
     4081 acggactaaa tgaatacatt ggtgaaacag aaaagaatgt tgcactgatt accgctatcg
     4141 acaaactcga taaaatcggg aaagaaggtg ttcaaaatga attgcgagaa aaaggattca
     4201 cggatgacca gatttcaaaa actgaaaaca tactagccat caccggtagt gctaccgata
     4261 aaatatctgc tttagaaaaa ttatttagcg gcgagaaagg caaaaaagga atcagtgaaa
     4321 taaaagaact atttgcaaaa atcgcaaatg ttggttcttc caatattcaa attgcttttg
     4381 acctcaccct tgctcgcgga ttaagttact ataccggcac tatctacgaa gtagtttcca
     4441 atgatgtgca aatgggctcc attttaggtg gcggacgtta tgatgacctc acaggtatct
     4501 ttggtttgcc aaatatgagt ggcgtaggta tttccttcgg actagatcgc atttatgatg
     4561 ttatcaccga attaaacaaa ttccctgaat ctgcaacctt acaaacacag gtattattta
     4621 ttgccttcga cgaatcggcc gaaactgcag cgctgcaata tgcacgtagt ttgcgcgatg
     4681 cggaaattcg ttgtgagtta tatcccgatg tgcaggcaaa aacagccaaa aaattcaaat
     4741 atgccgatga taagaaaatt ccttattgtg ctatcatcgg atcaaatgaa atggctgaac
     4801 aaaaggtgat gctaaaaaat atgcaaagcg gcgatcaaca attattatcc atcgaagaag
     4861 taatagaaat catcaccggt atccgcagca attttgttga tttgtctgaa tgatacatgt
     4921 aatgttgggt tcacgcagag gcgcagagac gcagagtcag caactattgc attcccttct
     4981 ctctcctctc tcctctctcc tctctccttt ctcctttctc ctccgaattt aaattggtca
     5041 ctcctcatta actcgcc
//
LOCUS       DAOQHY010000210         5056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_9479_length_5056_cov_5.876225, whole genome shotgun sequence.
ACCESSION   DAOQHY010000210 DAOQHY010000000
VERSION     DAOQHY010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9479_length_5056_cov_5.876225"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2256
                     /locus_tag="PLL28_13140"
     CDS             <1..2256
                     /locus_tag="PLL28_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70318.1"
                     /translation="EKLYLIEADTTMLFNSPLHQSTTALESGGVVYWNDPPMMWLDST
                     VYYWRITPDTSDGSEPLWRSSSFEFRIGDITGWNQSHYHQFLEDDYTNIQLQPSRNFE
                     FVPDVVTYEVSTGIYPTTNWTEVTCYQDGELFAVSSCASAGFVVVVADANSGQPWTTS
                     EVGTSNTGPYGDIYCSADPYERVIQFNTNTPERREFLYQFMMNTVPDSSYFICYSNNY
                     AEFNSWLDDTLIYGHSLFDAFVAYGAVDINSLAVFDYDRSYIFYAQKGNPASKFELIG
                     DPEGHKIEATFVIEGAWNTGDIVTPLIGPAASWDKAQWWLSSYDDPQSDTSMLSILGI
                     DTLGAMTVLADGLQSGDTSLTFIDAHTYPYLKLQLTASDDSLRTPVQLDRWRVIYDPV
                     PEAALNPNIAFSYDGDSLQQGPDVHLSMAVTNVSDYDMDSLLISVKVRDVANVLHPVP
                     YERQDSLLTDETMIATLGFSSSDFPTGNNTLIVEVNPKNDQPEQYHFNNIAYLPFFNA
                     ADTRDPLLDVTFDGIHILDGDIVSSKPTILINLKDENTFLALNDTSLIDIVIEYPDGS
                     EHSFPYDDIITRFYPADSSDLAENNSAKVELQPVLEADGIYILKIHGEDVTGNNAGDL
                     DYRISFEVINKAMISNVFNYPNPFSTSTRFVFTLTGSEVPEYFKIQIITVTGKVVREI
                     MRSELGELHIGNNITEFAWDGTDQYGDNLANGLYLYRVVTRLDGKALENFETGTDDYF
                     KSGYGKMYIAR"
     gene            complement(2253..3005)
                     /locus_tag="PLL28_13145"
     CDS             complement(2253..3005)
                     /locus_tag="PLL28_13145"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNF70319.1"
                     /translation="MTILQTILLIFAAIAGIGAAWILRKKTHSEFLSILLSFSGAYLL
                     GTVVLHLLPEIFHQEVSLLDHSNDAHITAVFVLAGFFIQLFIVQFTRGLEHGHMHLHD
                     HHTKSYVASVVFGLSVHAFMEGFPLSDAADYGVIEPVYKAILLHKIPEAFALATVLFF
                     SIRNKVITLSLLGLFTIMTPLGSYIGEVITSSHMLAYNRLIAIVCGSLIHISTTIIFE
                     ASGKAHKISLYKLGAILVGAGIALAGVWTESH"
     gene            complement(3029..3616)
                     /locus_tag="PLL28_13150"
     CDS             complement(3029..3616)
                     /locus_tag="PLL28_13150"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNF70320.1"
                     /translation="MFPEALQHIDEQLFLAINRQWGNQLFDTILPPVRDKMFWVPMYA
                     IIAVLIVYAWRWKGVLVVLCILANFALSDQVSSAVIKPAVGRLRPCNDPDFRDEVTLR
                     IDACGAGKSFTSSHATNTFAFAMICILLFRRRYKWVTPLALFWASMVSYAQVYVGVHY
                     PFDIIGGAILGVGMTIILYNIAIRYILKKYAPELI"
     gene            complement(3616..4356)
                     /locus_tag="PLL28_13155"
     CDS             complement(3616..4356)
                     /locus_tag="PLL28_13155"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNF70321.1"
                     /translation="MTGNNTQKWYNSWFGTPYYDLLYGHRDEQEASAFVATLITYLHP
                     NTTATILDAACGKGRHAILLADKGFTVTGIDLSAKNIREAQAFENEHLSFFKHDMRQI
                     FRIGYFDLICNFYTSFGYFDDPKDDVACLKAFAAGLKPGGKLVIDFLNIYHILQHLIE
                     DESRTIDGIRFTISKSYSNGFLHKKITISDNGNFSEYHEKVRALELKQFERYLMEAGL
                     HLENIFGDYRLSPFEKSSSDRLILIARK"
     gene            4438..>5056
                     /locus_tag="PLL28_13160"
     CDS             4438..>5056
                     /locus_tag="PLL28_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNF70322.1"
                     /translation="MTLIKSISGIRGTIGGKAGHNLTPEDIIRFTAAFGTWIKRTTGN
                     PVIVIGRDARISGDMINHLVIGTLQSIGIKVVDLGLSTTPTVEIAVPRENAGGGIIIT
                     ASHNPKNYNALKLVNHLGEFISGTDGQEVLRIAEEEEISYSDVDKLGEYQHKFYYIQK
                     HIDKILELDLVNRKAIKERNFRVVIDCVNSTGGISVPELLVALGVT"
BASE COUNT         1496 a         1048 c         1071 g         1441 t
ORIGIN      
        1 gagaaattgt atcttattga agcggacaca accatgttat ttaattcacc attgcatcaa
       61 tcaacaaccg cactcgaatc gggtggggta gtatattgga atgacccccc tatgatgtgg
      121 ttagacagca ccgtttatta ctggcgcatc accccggata cctccgatgg ttctgagcca
      181 ttgtggcgct ccagttcatt cgaattccga ataggtgata ttacaggatg gaatcaatca
      241 cactatcatc aattcctgga agatgattac accaatattc aattgcaacc gtctcgcaac
      301 tttgaatttg ttcctgatgt tgtaacatac gaagtatcaa caggtattta tccaactaca
      361 aactggacgg aggtaacttg ttatcaggat ggagaattat ttgctgtaag ttcatgcgcc
      421 agcgccgggt ttgtggtggt ggttgcggat gcaaattccg gtcagccctg gactacttcc
      481 gaagtcggaa caagtaatac cggtccttat ggcgatattt attgcagtgc cgatccttat
      541 gaacgcgtaa ttcaatttaa tactaatacc cccgaacgaa gagaattctt atatcaattc
      601 atgatgaata ctgtccctga cagtagttat tttatctgct attcaaataa ttatgccgag
      661 ttcaattcgt ggttggatga caccttgatt tatggacata gtttgttcga tgcgtttgtt
      721 gcttacggag cggtggatat taattcgctt gctgtttttg attatgacag atcttatatt
      781 ttttatgcac agaagggaaa tcctgcttca aaatttgaat tgatcggaga tcccgaaggt
      841 cataaaatag aagccacatt tgtgattgaa ggtgcatgga ataccggtga catcgtcact
      901 ccattgatcg gtccggctgc atcatgggat aaagcacaat ggtggctttc atcatacgat
      961 gaccctcaaa gtgatacatc aatgttatct attttgggta tcgatacatt aggtgcaatg
     1021 actgttcttg cagacggatt gcaaagtggc gatacgtctt tgacatttat agatgcgcac
     1081 acctatcctt accttaaatt gcaacttacc gcctcagatg attcactgcg tacacctgtt
     1141 caactcgaca gatggagagt gatatatgat cccgttcctg aggctgcgct gaatccaaac
     1201 atagccttta gttatgatgg cgatagtctg caacaaggcc ctgatgtgca cctcagtatg
     1261 gcagtgacca acgttagtga ttatgatatg gacagtctgc tcatttccgt taaggtgcga
     1321 gatgtagcaa atgtgttgca tccggtgccg tatgagcgac aagatagttt gttgacagac
     1381 gaaaccatga tcgccacctt aggattttct tcatctgatt ttcctacagg aaataatacg
     1441 ctcattgttg aggtgaatcc taaaaatgat caaccggagc aataccattt taataatatt
     1501 gcttacctgc catttttcaa tgctgcagat accagagatc cattgctgga tgtaacgttc
     1561 gatggcattc atattcttga cggcgatatt gtgagcagta aaccaactat tctcatcaac
     1621 ttaaaagatg aaaatacttt cctggcatta aatgacacga gtttaattga tatcgtaatt
     1681 gaatatcccg acggcagtga acacagtttc ccgtatgatg atatcattac cagattttat
     1741 cctgccgaca gttccgatct tgccgaaaac aatagtgcaa aggtggaatt gcaaccggta
     1801 ttggaggccg atggcattta tattttgaaa atacatggcg aagatgtgac aggcaataat
     1861 gcaggcgatc tggattatcg catttcattt gaggtgatca acaaagcaat gatcagcaat
     1921 gtatttaatt acccgaaccc tttcagcacc tctacacgat ttgtgtttac actcaccgga
     1981 agcgaagtgc ctgaatattt taaaatacaa atcatcaccg ttaccggaaa ggttgtcaga
     2041 gagatcatgc gcagtgagct cggtgaattg cacattggaa ataatatcac cgaatttgcc
     2101 tgggatggta ccgatcaata cggtgataac ttggcgaatg gattatacct ctatcgtgtt
     2161 gttactcgtc tcgatggaaa agcattagag aattttgaaa ccggaacgga tgactatttt
     2221 aaatccggtt acggaaagat gtatatcgca cgttagtgtg attctgtcca cacaccggca
     2281 agtgcaatgc ctgctcctac caagattgca ccgagtttat ataaactgat cttatgggct
     2341 ttaccgcttg cttcaaaaat aatagtggta gagatatgta tgagtgaacc acaaacgatg
     2401 gcaattagtc tgttgtaggc aagcatatgc gaagaggtaa tcacttcgcc aatataactg
     2461 cccagcggag tcatgatggt gaataaacct aacagactaa gtgtgatgac tttgttgcga
     2521 atggaaaaga agagcactgt cgccaaagca aaagcctcag gtatcttatg caataaaatc
     2581 gctttataaa ccggttcaat aacaccgtaa tctgctgcat cagaaagtgg aaatccttcc
     2641 ataaatgcat gcacacttaa tccaaaaaca acactcgcta cataagattt cgtgtggtga
     2701 tcatgcaagt gcatatgacc atgttcaagc cctcgggtaa attgaacgat aaataattga
     2761 ataaagaatc ctgccaacac aaacactgct gtgatatgcg catcattgga atgatccaag
     2821 aggcttactt cctgatgaaa tatttcaggc agtaaatgta aaacaacggt gccaagcaga
     2881 taggcgccac taaaagaaag cagtatgctt aaaaattcag aatgcgtttt ttttcgaagt
     2941 atccaggcag caccaattcc ggcaatggca gcaaaaatca ataatatggt ctgcagaatg
     3001 gtcatgcttt attacaatct tttcggcact aaattagttc cggcgcgtat tttttaagga
     3061 tatatcgaat tgcaatgtta taaagaatta tcgtcatccc cacaccaagt atcgctccgc
     3121 cgataatatc gaaagggtag tgcacaccta catacacctg tgcatagctg accatggaag
     3181 cccaaaataa ggcaagcggc gtcacccatt tataccttct tctaaacaaa agaatgcata
     3241 tcatggcaaa ggcaaaagtg tttgtagcgt gactgctggt aaagcttttt cctgcaccgc
     3301 atgcatcgat gcgcaaggtg acttcgtcgc gaaaatcggg atcattgcat ggccgcaaac
     3361 gtcctacagc aggttttata actgctgaac tcacctgatc gctgagcgca aagtttgcca
     3421 gtatgcataa aacaaccagc acacctttcc atcgccaggc ataaactatc aatacagcga
     3481 taatggcata catcggtacc caaaacattt tatcgcgtac aggaggcaat atggtatcaa
     3541 ataattgatt gccccattgc ctgttgatgg caagaaataa ttgttcgtcg atatgttgca
     3601 gtgcttcggg aaacattatt tacgtgctat taatattaat cggtcggagc tggatttttc
     3661 gaaaggcgat aatctataat ctccaaaaat attttcaaga tgtaatccgg cctccatcag
     3721 atatcgttca aactgtttca attccagtgc ccttactttt tcgtgatact ctgaaaaatt
     3781 accattgtcg gaaatcgtga ttttcttatg caaaaatcca ttcgaataac ttttggatat
     3841 cgtaaaccga atgccgtcaa tagtgcgact ttcatcttct atcaggtgtt gcaagatatg
     3901 atagatattg agaaaatcta ttaccagttt tccaccgggt tttaatcctg cggcaaaggc
     3961 tttcaaacaa gccacatcat ctttggggtc gtcgaagtag ccgaaagaag tatagaaatt
     4021 acatatcagg tcgaaatatc caatgcgaaa gatttgccgc atgtcatgtt taaaaaaact
     4081 gagatgctca ttttcaaaag cctgcgcttc gcgaatattt tttgctgaga gatcaatacc
     4141 tgttaccgta aatcctttgt ctgccaatag tattgcatgc cgacctttac cacaggccgc
     4201 atctaagatg gttgctgtcg tgtttggatg caagtaagta atgagtgttg ccacgaatgc
     4261 tgatgcttct tgctcgtcgc gatgtccata taacaggtcg taatacggcg tgccgaacca
     4321 ggaattatac catttctgtg tgttgttgcc tgtcattggt gtggacaaaa atagccgaaa
     4381 tcccgctatc cgacgtatcg gtcgccactg attttgatta cctttgcagg gttacttatg
     4441 acgttaataa aatccatctc cggtataagg ggaaccatcg gaggtaaagc agggcataat
     4501 ctcacccccg aagacattat cagatttacc gccgcattcg gcacctggat caaacgcact
     4561 accggcaatc cggtgatcgt gatcggcaga gatgcgcgca tcagcggtga tatgatcaat
     4621 catttggtga taggaacgct ccaatctatc ggtatcaaag tagtagacct cggcttgagc
     4681 actacgccaa cagttgaaat tgctgtccct cgcgaaaatg caggtggtgg tatcattatt
     4741 accgcttccc acaatcctaa aaattataat gcactcaaac tggtgaatca tttgggtgaa
     4801 tttatctccg gtactgatgg gcaggaagtg cttcgcattg cggaagaaga agagataagt
     4861 tactctgatg ttgacaagct aggtgaatac cagcacaaat tttattacat ccaaaaacat
     4921 atcgataaga ttcttgaact cgatctggta aatagaaaag caattaaaga acgcaacttc
     4981 cgcgtggtaa ttgattgtgt gaattctacc ggcggtattt ctgttcctga attattggtt
     5041 gctcttggtg ttactg
//
LOCUS       DAOQHY010000211         5053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_9490_length_5053_cov_3.891357, whole genome shotgun sequence.
ACCESSION   DAOQHY010000211 DAOQHY010000000
VERSION     DAOQHY010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9490_length_5053_cov_3.891357"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PLL28_13165"
     CDS             complement(<1..545)
                     /locus_tag="PLL28_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70323.1"
                     /translation="MLLMNNANSTEVLCISPTGQLGKACIELKLNTFDEACSYIAKLP
                     YRRNFHRDQVTGVLIDGCGTCSTKHRFLAALALEQAQQESNKINAGWKDIQLRIGVFE
                     MHANNTPAIAPVLHVAQLNAIPEAHCYLVINDMRCDFTSDKFTIPFQDTLLYETHVDP
                     INLFLMKDSIHKRILEQWAIN"
     gene            complement(529..1407)
                     /locus_tag="PLL28_13170"
     CDS             complement(529..1407)
                     /locus_tag="PLL28_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70324.1"
                     /translation="MQRVTVSLIFCCLLSSTLIAQLPAVDIYYAHISMSASGMQLTGL
                     TNITQRVGYDNQPSFSSDGNTILYTSIRDEKQADIYRYHIDSKLTEQLTNTVESEYSP
                     LLTADEKSFTTVRVEKDSTQRLWQIRIKDGKAKLYVEGVDSVGYYWPVNENIIPCFMV
                     TDTPSLMLVNKKANGGRTIAHNIGRCIKVLPGTYQITYLTKDSEQTFTIRAYNYITRE
                     FSVITEVPSISEDYVWTSDKKLLMPRGNAIWVYDTENPSAGWVKGCDIPTLTGKNIYR
                     MALSQDGKSLAFVADE"
     gene            complement(1457..1894)
                     /locus_tag="PLL28_13175"
     CDS             complement(1457..1894)
                     /locus_tag="PLL28_13175"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70325.1"
                     /translation="MNKLLQMPMVAVILKDNFSYMHLFLIAVLSVSMSTSAAGSATQF
                     ELNQLPGELQEVTYYYANGNRQQTGLLEDCLKTGEWISYSPEGTITARAYYDKGVKTG
                     KWRIYNAEGNLTYKICYENGIRKWAQAFDPAGELTAFSYTAEK"
     gene            1983..2357
                     /locus_tag="PLL28_13180"
     CDS             1983..2357
                     /locus_tag="PLL28_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HNF70326.1"
                     /translation="MAYELTGKVLEIFPTQEVSATFKKREFVIEKSESSGDRTFTDFI
                     KFQLIQDRCALADNFKIGDEVKVTFNIKGSKWEKEGRTNYFTNLDVWRMERVSGNNQA
                     PASQDTYQEPAPLPEVEDDLPF"
     gene            2366..2851
                     /locus_tag="PLL28_13185"
     CDS             2366..2851
                     /locus_tag="PLL28_13185"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNF70327.1"
                     /translation="MTLPVKLSPHRKKRYLRLFTALRKRKSAADDVLAENAHEAIFAA
                     TNCLDCANCCKTHPPLLEGPDIKRIAKHLHLSEASFMSNYLLLDEDGDWVFHTVPCPF
                     LESDNKCRIYDHRPKACREYPHTNRKKLYQISDLTLTNAEICPAVAPILDKLAAELHI
                     P"
     gene            2848..3105
                     /locus_tag="PLL28_13190"
     CDS             2848..3105
                     /locus_tag="PLL28_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70328.1"
                     /translation="MRIVGYIEHPVLKISVFSWSEKYIVKIEAGLFEQSYKFRQADFG
                     QWEDLKAFFDADMISDIMTTFKKMSADASASAKRLSSSGKA"
     gene            complement(3108..4820)
                     /gene="glgP"
                     /locus_tag="PLL28_13195"
     CDS             complement(3108..4820)
                     /gene="glgP"
                     /locus_tag="PLL28_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNF70329.1"
                     /translation="MINYSNWQLPYEPNPAFKQRTAYFCMEFGVHQALKIYSGGLGYL
                     AGSHMRSAYELRQNLFGIGILWRYGYYDQVRDDHGFMAVQFQKKLYTYLKQTDIAFTM
                     PINGKDVMVKVYYLAPEVFNSAPLFLLSTDFPENDEEVRQYTHRLYDRDHEIRIAQNM
                     ILGIGGAKVVELLGGADIYHMNEGHALAMTFYLFSKWKDVDEVRKHVVFTTHTPEKAG
                     NEEHNINDMERMGFFSGVPLDIIRHITKTEGDMLGYTPSALYMSKIANGVSKLHGEVS
                     REMWRDYPGICQITHITNAQQKKFWADRELNQAYINRDDMGLRVIKKELKRELFEVVA
                     NQTGKIFDPNVFTIVWSRRFAEYKRANLLMRNKERFLNFLNNPKYPVQVVWAGKPYPF
                     DHGAINTFNDIISFTMDRPNLAVLTGYEIELSYLLKRGADVWLNTPRRPREASGTSGM
                     TAAMNGAVNVSIMDGWIPEFAHHGENCFILPPADHYTMENVDIDNFDYENLMSVLENE
                     VVPMYYDNPEKWLNIVKKSMHDVYPYFDSDRMAVEYYQKVYQHPFSAVDKLLDSVFDN
                     NNNG"
BASE COUNT         1397 a         1111 c         1070 g         1475 t
ORIGIN      
        1 tggttgattg cccattgctc taatatcctt ttatgaatcg aatctttcat cagaaacagg
       61 tttatgggat caacatgagt ttcatataat aacgtatcct gaaatggaat tgtaaacttg
      121 tcagaagtga aatcacatcg catatcgttt attactaagt aacaatgtgc ttcaggaata
      181 gcatttagct gagctacatg caaaacaggt gcaattgcag gtgtgttgtt agcgtgcatt
      241 tcaaaaacgc ctattcgcaa ttgaatatct ttccagccgg catttatttt atttgattct
      301 tgttgtgcct gttccaaagc caaagcggca aggaatctat gttttgtgct acacgttcca
      361 catccgtcaa ttaacactcc ggttacttga tcacgatgaa aatttcggcg ataaggcaat
      421 tttgcaatat acgaacaagc ctcatcgaag gtgtttaatt tcagctcaat acatgctttt
      481 cccaattgac ctgttggaga aatgcagagc acctcagtag aattagcatt attcatcagc
      541 aacaaaggcc agcgattttc cgtcctgaga aagagccatt cggtatatat ttttccccgt
      601 caatgttgga atatcacatc ctttcaccca tcctgccgat ggattttcag tatcataaac
      661 ccatatcgca tttccacgtg gcatcaataa ttttttatcg gatgtccata cataatcttc
      721 gctgattgaa ggcacttctg taattacgga aaattcgcgg gtgatataat tgtatgccct
      781 gatggtgaat gtctgttccg aatcttttgt caggtaggta atttgatatg ttcctggtag
      841 gactttaatg catctgccga tattatgtgc aattgttctt ccgccattcg cttttttatt
      901 tacaagcatc agcgaagggg tgtctgttac cataaaacaa ggaataatgt tctcatttac
      961 cggccaataa tatcccacac tgtcaacgcc ttcaacatac aatttcgcct tgccgtcttt
     1021 gatgcgtatt tgccacaaac gctgcgttga gtccttttcg actctcactg tagtgaatga
     1081 tttttcatcg gctgtcaaca acggagaata ttcactttca acggtattgg taagctgttc
     1141 agtaagtttc gagtcaatat gataccgata aatatctgcc tgtttttcat ctcgaataga
     1201 agtatataaa atggtgttgc catctgatga aaatgatggc tgattatcgt aaccaacgcg
     1261 ttgtgtaata ttggtaagac ctgttaactg cataccactt gccgacattg aaatatgcgc
     1321 ataataaata tcaaccgccg gcaattgagc gattaaagta cttgagagta gacagcaaaa
     1381 aatcaaggag acagttacac gttgcatatg caatatttgc aacaaatgta acaaacctaa
     1441 atcgaggaaa ccggtattat ttttcggcgg tatatgaaaa cgctgttagt tctcctgccg
     1501 gatcaaacgc ctgagcccac ttacggatgc cgttttcata gcagatcttg taagtgagat
     1561 ttccctcagc attataaatc cgccacttac ctgtttttac acccttgtca taatatgctc
     1621 tagccgtaat agtgccttca ggagaataag agatccattc gcccgtcttc aagcagtctt
     1681 ccaataaacc cgtttgctgc ctgttgccat tagcatagta ataagtcact tcctgcaact
     1741 cacccggcag ttggttcagt tcaaattgag ttgccgagcc ggcagcagag gttgacatgg
     1801 acacagataa aacagcgatt agaaacaggt gcatatatga aaaattatcc ttcaaaatta
     1861 cagcaaccat gggcatttgc agcaatttgt tcatattaaa tttacattcc cgtcgctttg
     1921 agataacata cgcttaacat tgaaatttac agattgatta tctttggaga aatttttgtg
     1981 aaatggctta cgaattgaca ggcaaagtgt tggagatatt tcctacccaa gaggtttcag
     2041 ctacttttaa aaagcgcgaa tttgttattg aaaaatctga atcgagcggt gatcgcacct
     2101 ttaccgattt tattaaattt caattgattc aggatcgatg tgcattggcc gataatttca
     2161 aaattggcga tgaagtgaaa gtaacgttca acatcaaggg ttctaaatgg gaaaaagaag
     2221 gtcgtaccaa ttatttcacc aacctcgatg tatggcgtat ggagcgcgtg agtggaaaca
     2281 atcaggctcc ggcatctcag gatacttatc aggaacctgc accgcttcct gaagttgaag
     2341 atgatctgcc attttaattg accgtatgac acttccagtg aagctttcgc cacaccggaa
     2401 gaaacgttat ctacgtttat ttactgcact gcgaaaacgc aaatctgcag ctgatgatgt
     2461 attggcagaa aatgcccatg aagcaatatt tgctgctact aactgtcttg attgcgccaa
     2521 ttgctgcaaa acgcatccac ctctgcttga aggacctgat attaaacgca ttgccaaaca
     2581 tttgcatcta tcagaggcat cgtttatgag taattatttg ttgctggatg aggacggcga
     2641 ctgggtattt catactgtcc cctgcccttt tctggagtca gataataaat gtcgaattta
     2701 cgaccatcgc ccgaaagctt gtcgcgaata tccgcatacc aacagaaaga aattgtatca
     2761 aatcagtgat ctgaccttaa caaatgcaga aatctgccct gcagttgccc cgattttgga
     2821 taaattggca gcagaattac atattccatg aggatagttg gctacattga acacccggta
     2881 ttgaaaatca gcgtatttag ctggagtgag aagtatatcg taaagatcga agccggccta
     2941 ttcgaacagt cctataaatt ccgacaggct gatttcgggc aatgggaaga tcttaaagca
     3001 ttcttcgacg ccgatatgat atcagacatc atgaccacct tcaagaaaat gtcggccgat
     3061 gcttccgcat ctgcaaaacg ccttagcagc agcggtaaag cctgatccta gccgttatta
     3121 ttattatcga aaactgaatc gagtagctta tctacggcgc tgaaagggtg ctgatatacc
     3181 ttttgataat attcaaccgc catgcgatcg ctatcgaaat aaggatatac atcgtgcatg
     3241 ctcttcttaa cgatattcaa ccatttttca gggttgtcgt aatacatcgg cacaacctca
     3301 ttctccagca ccgacatcag gttttcataa tcgaaattgt cgatgtctac gttttccatt
     3361 gtgtaatggt cggcaggcgg caggataaag caattttcac catgatgagc aaattccgga
     3421 atccagccat ccatgatgct cacgttgact gctccgttca ttgctgctgt catgccggat
     3481 gttccagaag cttcccttgg gcgacgaggt gtattcaacc acacatcagc accgcgtttc
     3541 aacagataac tcaactctat ttcataaccg gttagcaccg ccaggttagg gcgatccatg
     3601 gtaaatgaga tgatatcatt aaaggtgttg atggcaccgt gatcgaaggg ataaggcttt
     3661 cctgcccaaa ccacttgaac gggatatttc ggattattga ggaaattgag aaagcgttct
     3721 ttgttgcgca tcaacaggtt tgcacgtttg tattcggcga atcggcgcga ccaaacgata
     3781 gtaaacacat tcgggtcgaa gattttgccg gtttggttgg caacaacctc gaagagttcg
     3841 cgtttaagct cttttttgat aacgcgcagt cccatgtcat cgcgattgat atacgcctga
     3901 ttgagttcac ggtcggccca gaatttcttc tgctgtgcat tggtgatatg cgtaatctga
     3961 cagataccgg gataatcgcg ccacatttcg cgacttactt cgccgtggag tttgctcacg
     4021 ccgttagcaa ttttgctcat atagagggct gatggcgtat atccgagcat gtcgccttcc
     4081 gttttcgtaa tatggcggat gatatcaaga ggtacaccgc tgaaaaatcc catgcgctcc
     4141 atatcgttga tattatgctc ctcatttccg gccttttcag gggtatgtgt ggtgaaaaca
     4201 acatgcttac gcacttcatc aacatccttc cacttactga acagatagaa agtcattgcc
     4261 agcgcgtgtc cttcattcat atggtagatg tctgcaccac caagcaattc aactactttg
     4321 gcgccgccga tacccagaat catgttctga gcaatgcgaa tctcgtgatc gcggtcgtag
     4381 aggcggtgag tatactgacg cacttcctcg tcgttttcag ggaaatcggt gctaagtaag
     4441 aaaagcggtg ctgaattgaa tacttcaggt gccaggtaat acactttcac catcacatcc
     4501 ttgccattga taggcatggt gaaggcaata tcagtctgtt taaggtaggt gtaaagcttc
     4561 ttctggaact gaacagccat aaatccatga tcatcgcgca cctgatcata gtaaccatat
     4621 cgccacaaaa tacctattcc gaagaggttc tggcgcaatt catatgcact gcgcatgtgc
     4681 gatcccgcaa ggtagccaag gccgccgctg tatattttga gtgcctggtg aacaccaaat
     4741 tccatgcaga aataagcagt tcgttgcttg aaggccggat ttggttcata aggcagttgc
     4801 caattgctgt agttaatcat cgttcgttct cgttgtcagg tgcgaatata tagaaaacgt
     4861 agattttgca atttgagcta atgtggattc gtgaatcgtg aatcgtgact cgtgaatcgt
     4921 gaatcgtgaa tcgtgaatgg tgagtggtga gtggtgaatt tctttgcggc tttgcctctt
     4981 cgctgtcttt gcgcgaaccc cgcctcaccc cccgccccac acctcctttc gtcggagcgg
     5041 caagctctcc atc
//
LOCUS       DAOQHY010000212         5033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_9556_length_5033_cov_5.969466, whole genome shotgun sequence.
ACCESSION   DAOQHY010000212 DAOQHY010000000
VERSION     DAOQHY010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9556_length_5033_cov_5.969466"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..151
                     /locus_tag="PLL28_13200"
     CDS             5..151
                     /locus_tag="PLL28_13200"
                     /inference="COORDINATES: protein motif:HMM:NF014033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70330.1"
                     /translation="MVTHLAENEHVEPIILIYLNRLSDYLFVAARKIAHDLGIDEVAW
                     RPRG"
     gene            314..535
                     /locus_tag="PLL28_13205"
     CDS             314..535
                     /locus_tag="PLL28_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HNF70331.1"
                     /translation="MYWTLELASYLEDAPWPATKDELIDYAIRSGAPIEVIENLQELE
                     DEGEFYEGIEDIWSDYPTHDDFYFNEDEY"
     gene            complement(596..1648)
                     /gene="queA"
                     /locus_tag="PLL28_13210"
     CDS             complement(596..1648)
                     /gene="queA"
                     /locus_tag="PLL28_13210"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNF70332.1"
                     /translation="MKLSKFNFELPKDIIAQHPTAERDQSKMMVVHRKTGKIEHKKFR
                     DILGYFDENDVMVLNNTKVFPARLYGRKEKTGAKIEVFMLRELNHEMHLWDVLVDPAR
                     KIRVGNKLYFGDDDMLVAEVVDNTTSRGRTIRFLHDGTAEELRRILEIMGETPLPKYI
                     KRKPEESDRERYQTVFAKHEGAVAAPTAGLHFSRELMKRLEIKGINFAELTLHVGLGT
                     FRSIDVEDLSKHKMDAEYFKIDQKCVNVVNKALDENRRVCAIGTTSMRACESAVAATG
                     RLKTEEGWTNLFIHPPYDFSICNSLVTNFHLPKTSLVIMTAAFMGYDLTMEAYKVAMK
                     EKYRFFSYGDAMLIVD"
     gene            1757..3028
                     /locus_tag="PLL28_13215"
     CDS             1757..3028
                     /locus_tag="PLL28_13215"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF70333.1"
                     /translation="MLGIFFKTIAETMGLVFQELRNNKLRTSLSLLGVSIGIFCIIAV
                     LSAVDSLKRNMESGLNKLGTDMIYITRWPWQFSNEEYTWWEYVKRPTMKYDEFQQLSD
                     KLTLAKAVAIETWLSPQEVKFMDHTLQNASISGVTSQYDEVYTMNFMSGRYFTDGESV
                     GGSEVAILGYEVAQELFPGGLQPVGKYFWVRANGSQFKLRVIGVLEKEGESIIDISND
                     NSIIIPFNYIRRIVDMNSPMIDPMIEVRPKEGVTADEIKDEIYPIMRNIRNLRPAEQN
                     DFAVNEITLAADALDNLFAALNMVGMIIGGFSILVGGFGIANIMFVSVKERTNVIGIK
                     KSLGAKNYLILTEFLIEAIVLSLIGCLFGLLLVWVLMLVAENLSDFGFILSWTNIAIG
                     AIISISLGVVAGIVPAITASRMNPVEAIRSR"
     gene            complement(3040..4035)
                     /locus_tag="PLL28_13220"
     CDS             complement(3040..4035)
                     /locus_tag="PLL28_13220"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HNF70334.1"
                     /translation="MATSKPDIKKLFRQFSDMRVMIVGDVMLDKYIYGQVDRISPEAP
                     VPIVDVRSATARPGGAANVAVNIAALGATPYLFSVTGKDHDADLLCELMEEQHIDPQY
                     LLHAPDRITTSKMRVVAKQHQMLRIDHEMQDELQEETAITFLQNVVGFIKKKKPHVVI
                     LEDYDKGVLSASLITEIISKCHTLHIPVVVDPKKKHFFHYKGCTLFKPNLRELRDSLD
                     GNPSTATISDMKLAVAALQTLMPHHISMVTLGEYGVFIQEGKKSRIIPAHHRNIADVS
                     GAGDTVISVAALCLAAGADIFTTAAISNLAGGLVCEHAGVVPVDKQQLLKEALAN"
     gene            complement(4038..>5033)
                     /locus_tag="PLL28_13225"
     CDS             complement(4038..>5033)
                     /locus_tag="PLL28_13225"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNF70335.1"
                     /translation="VAGYLEVRQAVSEKFLRENGLHYSPEQIVISTGAKQSIINAVLC
                     LVNPGDEVILPTPFWVSYSAMVKLADGVIKEIPTSIASDFKITPDQLEAAISSKTKLI
                     IFSSPCNPTGSVYTKEELFDLAKVLEKHPHVYVISDEIYEHINFTDKHASMAQFDFMY
                     DRVITVNGLSKAFAMTGWRLGFIGAPVWIAKACDKMQGQYTSATCSITQRATIAALTG
                     DIEPMLVMKDAFLRRRDLVYGLLKAIPGLKVNNPQGAFYFFPDVSNYFGKQYQGQIVN
                     NADDMALYLLNEGNVSVVTGSAFGDQNCIRLSYATADEKLIEAMKRMADALSKLQ"
BASE COUNT         1432 a         1025 c         1046 g         1530 t
ORIGIN      
        1 acgtattgtt acgcatctgg ctgaaaatga acatgttgaa ccaataatcc ttatctactt
       61 aaaccgtttg agcgactatc tttttgtggc agcacgcaaa attgcacatg atctgggaat
      121 agatgaagta gcatggcgac cgcggggata gtgctgtatt cttgtatata cagatatttt
      181 ttcaatcctg taacccttta ctggtaagca tttcagcgat ttttcgcttt gacgcacaga
      241 ttgtttacaa tacaagttgt aaaacctgaa aaaaggtata tttttgcgcc ctaataaaat
      301 cgcggactta attatgtatt ggacactgga actagcctca tatcttgaag atgcaccatg
      361 gcctgccacc aaagatgaat tgattgacta tgctattcgt tcgggagcac ctattgaagt
      421 gatcgagaat ctgcaggaat tagaggatga aggagaattt tatgaaggta tcgaggatat
      481 ctggagtgat tatcctaccc acgacgactt ttatttcaac gaggatgaat attgatccgg
      541 ttgaaacaaa aaaaaaatcc tgctcatcgg caggattttt ttttgaggat atctcttagt
      601 caacaatcag catggcatca ccataactga aaaagcggta tttttctttc attgccacct
      661 tgtatgcctc catggtaagg tcataaccca taaatgcagc agtcataatg actaggcttg
      721 ttttgggtag atggaaattc gtaaccaaag agttacaaat gctgaaatca tagggaggat
      781 gaatgaagag gttggtccat ccttcttctg ttttgagtcg tcctgttgca gcaactgcgc
      841 tttcacaggc tctcattgaa gttgttccga tggcacatac tctgcgattt tcatcgagag
      901 ctttgttaac cacattcaca catttctgat ctattttgaa gtattcggca tccattttat
      961 gcttgcttag gtcttccaca tcaatgctgc ggaaagtgcc caaacctacg tggagtgtga
     1021 gttctgcaaa attaataccc ttgatctcca agcgtttcat taactctcga ctgaagtgta
     1081 aaccggcagt tggagcagcc accgctcctt catgtttcgc aaaaacggtc tgatagcgct
     1141 cgcggtcaga ttcttcgggt ttgcgtttga tatattttgg aagtggtgtt tctcccatga
     1201 tctcgagaat acgacgcaat tcttcagcag tgccgtcatg taaaaagcgg atggtgcgac
     1261 cgcgagaagt ggtgttatca accacctcgg caaccagcat atcatcatcg ccaaaataca
     1321 atttgttgcc tacacgaatc ttacgggctg ggtctaccag aacatcccat aaatgcatct
     1381 catggttcaa ttcacgaagc atgaatacct caatcttggc acctgtcttt tctttgcggc
     1441 catacaatct tgccggaaaa accttggtat tattcaatac catcacatcg ttttcatcga
     1501 aatagccaag aatgtctctg aattttttgt gttcgatctt gcctgttttc ctgtgtacca
     1561 ccatcatctt gctttgatcg cgctcggcag taggatgttg ggcaataatg tctttgggaa
     1621 gctcgaaatt gaatttggat agtttcataa aacttacggg ttttagttat tgtgagcctg
     1681 aagttaggcc gacaagggcg caaaattaac cattctataa ctaattcacc gcgtttatct
     1741 gataactttg tggcagatgc tagggatttt tttcaaaacg attgcagaaa cgatgggttt
     1801 ggtgtttcag gaacttcgaa acaacaagct gcgtacatca ctttccctgc ttggtgtatc
     1861 tataggtatc ttctgtatca tagctgtgtt gagtgccgta gactcgctta aacgcaatat
     1921 ggaaagcggg ctgaataaat taggcaccga catgatttat atcacacgtt ggccctggca
     1981 gttcagtaat gaagaatata catggtggga atatgtgaaa cgtccgacca tgaagtatga
     2041 tgaattccag cagctcagcg ataaattgac acttgcaaaa gcagttgcca ttgaaacatg
     2101 gttgtcgcca caagaggtga aattcatgga tcatactttg caaaatgctt ccatcagcgg
     2161 tgtaaccagc cagtatgatg aagtgtacac catgaatttc atgagtggaa gatattttac
     2221 agatggtgaa tctgttggtg gttctgaagt tgcgatattg ggttatgaag tggcgcaaga
     2281 actatttcct ggtggacttc aaccggttgg gaaatatttt tgggtgcgcg caaacggctc
     2341 tcaatttaaa ttgcgcgtga ttggtgtatt agaaaaagaa ggggagagca tcatcgatat
     2401 ttctaacgat aattctatca tcattccttt taattatatc cgtcgcattg tggatatgaa
     2461 tagtcctatg atagatccta tgatagaagt gcgtcctaaa gaaggtgtta cagccgatga
     2521 aataaaggat gagatctatc ccatcatgcg taatatcaga aatctccgac ctgctgaaca
     2581 aaatgatttt gcagtgaacg aaattacact tgcagccgat gcacttgata atctgtttgc
     2641 agcgctcaat atggtgggca tgatcatagg tggatttagc attctggttg gtggatttgg
     2701 tattgccaat attatgttcg taagcgttaa agaaagaact aatgtaattg gcataaaaaa
     2761 atcgctcggc gcaaaaaatt atttaattct caccgagttc ctaattgaag caattgtact
     2821 ttccttgatt ggctgtttgt tcggattgct attggtttgg gtgcttatgt tagttgcaga
     2881 aaatctatct gacttcggat ttattttatc ctggacaaac atcgcaattg gagcaattat
     2941 ctccataagt ttgggtgtag ttgcaggtat tgttcctgcc attaccgcaa gtagaatgaa
     3001 tcctgtagaa gcgattagat ctcgctgaca atagtataat taattcgcta aagcttcctt
     3061 caacaattgt tgcttatcta caggcacaac acccgcatgt tcacatacta atccgccggc
     3121 aagattactg atagctgcgg tagtaaatat atcagcacct gcagcaagac ataatgcagc
     3181 aacacttatc acagtatctc cggcgccact cacatcggca atattgcgat ggtgtgcagg
     3241 tatgatgcga gattttttcc cttcctgaat aaatacacca tattccccca atgtaaccat
     3301 agaaatatga tgcggcataa gcgtttgcaa ggctgcaacc gcaagtttca tatcagatat
     3361 ggttgctgtt gacggatttc catccaggct gtcgcgcagt tctcgaagat ttggtttaaa
     3421 aagggtacat cccttataat ggaagaagtg ttttttcttt ggatctacaa caacaggaat
     3481 atgcaaagta tgacatttgc tgattatttc tgtaatcaat gatgccgata aaacaccttt
     3541 atcataatct tccagaataa ctacatgtgg ttttttcttt ttgatgaatc caacaacatt
     3601 ttgcaggaat gtgatggcgg tttcttcttg caattcatcc tgcatttcat ggtcgattcg
     3661 cagcatttga tgttgcttgg caaccacacg catttttgat gttgtgatgc gatcaggagc
     3721 atgcaaaaga tattgcgggt cgatatgttg ttcttccatt aactcgcata acaagtcagc
     3781 atcatggtct ttaccggtaa cggaaaacaa atacggtgtg gcacctaatg cggcaatatt
     3841 caccgcaaca ttagccgctc ctcccggtct tgcggtggca ctgcgtacat caacaattgg
     3901 aaccggtgcc tccggagaaa tgcgatcaac ctgaccatag atatatttat ccaacatcac
     3961 atcaccgaca atcattacac gcatgtcgct gaactgtcgg aatagttttt ttatatctgg
     4021 ttttgatgtc gccattgtta ttgcaatttt gaaagtgcat cagccattcg tttcattgct
     4081 tcaatgagct tttcatctgc ggtagcataa ctcaaacgaa tacaattctg atcgccaaaa
     4141 gcgcttcctg ttaccacaga aacattgcct tcattcaata aatataaggc catgtcatcc
     4201 gcattattga caatctgccc ctgatattgt ttcccgaaat aattgcttac atcagggaaa
     4261 aaataaaatg caccttgcgg attgttgacc tttagtcccg gtattgcttt caacaaacca
     4321 taaacaagat cgcggcgacg taaaaatgca tctttcatga ccaacatcgg ttctatatct
     4381 ccggtcagcg cagcaattgt ggctcgctgt gtgatactac aagttgctga ggtatattga
     4441 ccttgcattt tatcgcaggc cttagcaatc caaacaggcg caccaataaa tccgaggcgc
     4501 cagcctgtca tggcaaatgc tttagataat ccgtttactg ttattacccg gtcatacatg
     4561 aaatcaaact gtgccatgct tgcatgttta tcggtgaagt tgatatgttc atatatctca
     4621 tcactgatca cataaacatg cggatgtttt tctaaaactt tggcaagatc gaatagctcc
     4681 tccttcgtat aaacagaacc ggttggatta catggcgaag aaaaaataat caattttgtt
     4741 ttggaggata ttgctgcttc cagttgatcc ggagtaattt taaagtcgct tgcaatagaa
     4801 gttggaattt cttttataac accatcagca agttttacca ttgcagaata actaacccaa
     4861 aatggggtag gtaatatcac ttcatcgccg ggattcacca ggcataaaac agcattaata
     4921 atcgattgct tagcaccggt tgaaataaca atttgttcag ggctataatg caatccattt
     4981 tcacggagaa atttttcaga aactgcctga cgcacttcaa gatatcctgc tac
//
LOCUS       DAOQHY010000213         5033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_9557_length_5033_cov_5.254721, whole genome shotgun sequence.
ACCESSION   DAOQHY010000213 DAOQHY010000000
VERSION     DAOQHY010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9557_length_5033_cov_5.254721"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PLL28_13230"
     CDS             complement(<1..866)
                     /locus_tag="PLL28_13230"
                     /inference="COORDINATES: protein motif:HMM:NF015378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNF70336.1"
                     /translation="MKKLLIISGIAWMCSIVNVKAQSMALPLQITNKYQLSIEPGSTR
                     EWISFRRDAPFQALTIFEQAPDLLGLREQDEFRKFSERTDAAGNKHYKFAQYVNGVRV
                     EGIEYYTHERNGRFHLMNGDVVNNLQLSSTPSISKEQAIASALAQFPATKYLWEDPRA
                     EAAFKQKNNNPNASLYPDPELLFVKIDPKGPQVADNYILAYRMYVFAVEPHIAEYVYV
                     DATTGAFIRNRSLEISCNPSSMATTFNGTQTVNTDYRTEECGSTYTTETSYFSIDDCT
                     LGSEIRSYYSAS"
     gene            complement(891..2732)
                     /locus_tag="PLL28_13235"
     CDS             complement(891..2732)
                     /locus_tag="PLL28_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70337.1"
                     /translation="MALWNKVTLQQHQLYQHAKHVMTAENMEIRNLKNIIMKKQLFTS
                     IFTLSALFGVAHAQTNTFPTTGSAGIGTITPASSAILDIQSTTQGMLAPRMTKAQRDA
                     IVSPTTGLLIYQTNSSPGFYYYTGATWSAMAAKGANTSLSNLAASTSINTALTPNANN
                     TLDLGSTTNNWNELYVNTIKFMDGTTQITAGGGGGGGVGGSGTISYLPKFTSATTLGN
                     STIYDNGGNVGINTTTMVGSANFVSKSNSVTGYGGMYVEMGGTSGRKPFYGYSLSGIA
                     KAWTYFDEATNQYRVNNNGDRMVIDNSGNVGIGTTTPSNLLHVETSGTVTPVKFVKPW
                     TGTGTTDFFLLEVSNNYNFGYGSGINAYGGKMGIKGSAFNGDQIGYGVYGYGFGGTGT
                     TYGVYGTAGSSGSAIGVYGTTTSGSSQWAGYFVGRGYFSDNLGIGTSTPSTKLHVIGG
                     TDVELATGGFATFGATTATNIAIDNNEIMARNNGATSTLYLNNDGGNVSMCYASGSVM
                     IGASVPATGYLLSVDGKVMCEELKVQMSESWPDYVFADNYKMPTLYELEASIKANKHL
                     PGIPSAAEVAENGISVGEMQTNMMEKIEELTLYIIELQKQIDTLKQQ"
     gene            2780..4207
                     /locus_tag="PLL28_13240"
     CDS             2780..4207
                     /locus_tag="PLL28_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70338.1"
                     /translation="MENTNLQQLLQTLTKSEWREFGDFLSSPFFNKNEKFIRFYHQIN
                     PYQPGFVISEPEKQRIYIETTQSKAWNDAGYRNLCSDFLELLQSFITQLVIASGSSQK
                     GFYLNMELLQRGQLALVEKNLKKISSYLKTDSIDIGERLRTEVWLADLHTLLNIRMNR
                     SRIHHASQFMINDTSFRTNTELLLLKVFITFHNYITATQNQNEPFDKKRIKPFLQLYE
                     AFEPFEHVEVALNYFLLRMVSEGADDNYYKTKELFISVLDKLHNKDKSNMMTSLLNFV
                     NGKVGSDESWREEVFALHDLKLTKRLWSNHSDLGYVSLFTAVQNALQMNRITYAEDIL
                     EQYSPFLNHVIRDSIYHLSKGFIALFKGDYSFAHQCIQKVETENNLLKYEIRVLQLLV
                     YFEQKEWNLLASALESFRQFIQYNKPNISPEITDQYLTFVKQMQQLMKLPPAPSQKQR
                     DQLIKTLRTDGHAYFKSWLLRKAQN"
     gene            complement(4208..>5033)
                     /locus_tag="PLL28_13245"
     CDS             complement(4208..>5033)
                     /locus_tag="PLL28_13245"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HNF70339.1"
                     /translation="CIMSCSDSTNTAEKVNIPAAKGKLFIIGGGDRGDSLMQAMLDAA
                     GWEKGDLITAITLPSGWGDSAYIWLNNDFKKLTGQNCVKFDSAAVHVPAKLDSLRMSK
                     VIFISGGDQNKMMQLIKGTDIKKAIKEAYMNGATISGTSAGASIQSEKMITGDGLIDT
                     VYDVTFDVLWNGNIELVEGLGLLDSVIIDQHFVVRSRHNRLLSAVIENPGYQGVGIDE
                     GTAILVSGDTATVYGASQVLTFTNPASITSPKDSLLNGRDIRVSIYLPGEKFAIKR"
BASE COUNT         1443 a         1094 c          972 g         1524 t
ORIGIN      
        1 tatgaggcac tgtaatagct acgaatttct gaaccgagcg tgcaatcgtc gatggaaaaa
       61 tagctggttt ccgtagtata ggtagatccg cattcttcag ttctataatc agtatttacg
      121 gtttgcgtac cattgaaagt tgttgccatt gacgatggat tacaactaat ttccagggaa
      181 cgattacgga tgaaggcgcc ggtagttgca tcaacatata catattctgc aatatgcggt
      241 tctacggcaa aaacatacat gcgatatgcc aaaatataat tgtcggcaac ctgcggtcct
      301 tttggatcta ttttcacaaa caacaattcc ggatcaggat acaatgatgc attcggatta
      361 ttattttttt gtttaaacgc tgcttctgct ctcgggtctt cccacaaata tttagttgca
      421 ggaaattgag ctaatgcact tgcaattgcc tgctctttgc tgattgacgg ggtggatgac
      481 aactgtaaat tgttcaccac atcgccattc atcaaatgaa atcttccatt tcgttcatgc
      541 gtataatatt caattccttc aacgcgaaca ccgttaacat attgggcaaa cttatagtgc
      601 ttatttccgg cagcatcggt gcgttcagag aatttgcgaa attcatcctg ttcgcgcaat
      661 cccaataaat caggagcttg ttcaaaaatg gtgagtgcct gaaaaggtgc atcgcgacga
      721 aaagaaatcc attcacgagt gctaccgggt tcaatactca gttgatattt atttgttatc
      781 tgtaaaggca atgccatgct ctgcgccttt acattgacga tactacacat ccatgcaata
      841 ccgctaatga tcaataattt tttcatgatc gtaagttgat atcgatgaaa ttattgttgt
      901 ttaagcgtat cgatctgctt ctgcaattcg ataatatata aggtaagttc ttcgatcttc
      961 tccatcatat ttgtttgcat ttcgccaaca ctgatgccat tttctgcaac ttctgctgct
     1021 gaaggaatgc ccggaagatg tttatttgct ttaattgatg cttccaattc gtagagtgtc
     1081 ggcattttat aattatcggc aaaaacataa tcaggccagc tttcgctcat ttgcactttc
     1141 aactcttcgc acatcacttt gccatccaca ctcaacaagt atcccgttgc aggtacactt
     1201 gcgccaatca taactgaacc acttgcataa cacatactta cgttgccacc atcattgttg
     1261 agatataaag tagaagttgc accattgttg cgagccatga tttcattatt gtcgatagct
     1321 atattagttg cagtggtggc tccgaatgta gcaaatcctc ctgttgcgag ttctacatcc
     1381 gtacctccta tcacatgtaa ttttgttgaa ggcgtacttg ttccaatgcc cagattatcg
     1441 gaaaaatatc cacggccaac aaagtaaccg gcccattgcg acgatccgct tgtggtagtg
     1501 ccataaacac cgatggctga accgctggag ccggcagtgc catatacccc ataggttgta
     1561 cctgtgccac cgaatccata tccataaaca ccatagccaa tctgatcgcc attaaatgca
     1621 gatcctttaa tacccatttt accaccatac gcattgatcc cgctaccgta accgaagttg
     1681 taattattac tcacctccaa caaaaagaaa tcagtggtac ctgttcctgt ccatggcttt
     1741 acaaatttta caggagtaac agttccggat gtttcgacat gtaataaatt gcttggagtt
     1801 gttgttccga tacctacatt acctgaatta tcgataacca ttctgtcgcc attattgttg
     1861 acgcggtact gattagttgc ctcatcaaaa tatgtccatg cttttgcaat gccacttagt
     1921 gagtaaccat aaaacggttt tcttccgctg gtgccgccca tttcaacata cataccgcca
     1981 tatccggtaa cggagttaga ttttgaaacg aagttcgccg agcccaccat agtggtagta
     2041 ttgataccaa cgtttccgcc attatcgtaa atagtagaat ttccgagcgt tgtagcggaa
     2101 gtaaattttg gaagatagct tatagtacca cttccgccta ctcccccacc gcctccgcca
     2161 cctgcagtaa tttgtgtggt gccatccatg aatttaatgg tattgacata cagttcattc
     2221 cagttgttag tggttgaacc gaggtcgagg gtattgttag cattgggtgt taatgctgta
     2281 ttgatactag tagatgccgc caaattggaa aggctggtat tggcaccttt tgctgccatt
     2341 gccgaccagg tagcgccggt gtaataatag aatccgggac tgctgttcgt ctggtatatc
     2401 aataatcccg tagtaggaga aacgatagca tcacgctgag cctttgtcat gcgaggtgcc
     2461 aacataccct gtgtggtgct ttggatgtca aggatagcac tcgaggcagg agtaatggtt
     2521 ccgataccgg cacttcctgt ggttgggaat gtgtttgtct gtgcatgggc tacgccaaac
     2581 aatgccgaca aagtaaagat tgaagtaaat aattgttttt tcatgataat gttttttaag
     2641 tttctgattt ccatgttttc agctgtcatc acatgttttg catgctgata tagctgatgt
     2701 tgctgcaaag tgactttatt ccacagggcc ataccagtag aaattttttc atttgtagca
     2761 gatttggcat ctttaaggtg tggaaaacac gaatttgcag cagttgttac aaaccctcac
     2821 caagagcgag tggcgtgagt tcggggattt tttgtcatca ccttttttta ataaaaatga
     2881 gaagttcata aggttttatc atcaaatcaa cccataccaa ccgggttttg tcatctctga
     2941 gccggaaaag cagcgcattt atatcgaaac cacccagtcc aaagcttgga atgatgccgg
     3001 ctatcgaaat ctatgctccg attttcttga gttattacaa tcattcatca cacaattagt
     3061 aattgcctca ggaagctcac aaaaaggatt ttatctaaat atggagctcc ttcaacgtgg
     3121 acaattggcg ctggtagaaa aaaatttaaa gaaaataagc agctatttga aaaccgatag
     3181 catagatatc ggtgagcgat tgagaactga ggtttggtta gcagatttac atacacttct
     3241 gaatatccgc atgaacagaa gtcgcataca tcatgcctct caatttatga tcaatgatac
     3301 ttctttcaga actaataccg aattattgtt gcttaaagta tttattactt ttcacaatta
     3361 tataacagca actcaaaatc aaaatgaacc tttcgataaa aaacgtatca aaccctttct
     3421 gcaattatac gaagcatttg aaccttttga acatgttgag gttgccttaa attattttct
     3481 cctcaggatg gtttctgaag gtgcagatga caactattat aaaactaagg aattatttat
     3541 ttcagtgctg gacaagttac acaacaagga caaaagtaac atgatgacca gcttgttgaa
     3601 tttcgtaaat ggaaaggttg gatccgatga aagctggcgt gaagaggtat ttgcattaca
     3661 cgacctgaaa cttacaaaac gattgtggtc gaatcatagt gacctgggtt atgtcagttt
     3721 gtttactgct gtgcagaatg ccttacaaat gaatagaatc acctatgccg aggatattct
     3781 ggagcaatat tcaccgttct tgaatcatgt aattcgcgat agcatttatc atttatcgaa
     3841 aggatttatt gctttgttta aaggcgatta ttcatttgca catcaatgca ttcagaaggt
     3901 ggaaactgaa aataatctgc ttaaatacga aattcgtgtt ttacaattgc tagtatattt
     3961 cgaacaaaag gaatggaatt tacttgcctc tgcattggag agtttcaggc aatttattca
     4021 atacaataag ccaaatatca gccccgaaat aaccgaccag tatctcacct ttgtcaagca
     4081 gatgcaacaa ttaatgaaac ttccgcctgc gccctcacaa aagcaacgtg atcaacttat
     4141 taaaacactg cgcacggatg gacacgccta tttcaaatca tggctgctgc gaaaagcgca
     4201 aaattgatca gcgtttgatg gcaaatttct ctccgggcag gtaaatagaa acccgaatat
     4261 cacgcccatt caataaacta tcctttggcg atgtaattga tgcaggattt gtaaacgtca
     4321 gcacttggct tgctccgtat actgttgcgg tatcgccgga aacgagtata gcagtgcctt
     4381 catctatacc aacgccctga taacccggat tttctatcac tgcactcaat aatcgattat
     4441 gcctgctcct gacaacaaaa tgctgatcaa tgattacaga atccagtaaa ccgagacctt
     4501 caacaagttc aatatttcca ttccacaaca catcaaaggt tacatcgtac acagtgtcga
     4561 tcaatccatc accggtgatc attttttcac tttgaatact tgctcctgca cttgtaccgg
     4621 aaatggtggc tccattcata taagcttcct ttatagcctt tttgatatca gtgcccttta
     4681 tcagttgcat cattttgttt tgatcaccac cactaataaa aatcactttt gacatacgca
     4741 gcgaatccag ttttgccggc acatgaacgg cagcagagtc aaactttaca cagttttgtc
     4801 ctgtcagttt tttgaaatca ttgttcaacc agatataggc actgtcgccc cagccgctcg
     4861 gcaaggtaat tgctgtgatc aaatcaccct tttcccatcc tgcagcatcc aacatggctt
     4921 gcatcagact gtcgcctctg tcaccaccac ctataataaa gagttttcct tttgccgcag
     4981 gtatatttac tttctctgca gtgttcgtcg aatcggaaca agacatgatg cac
//
LOCUS       DAOQHY010000214         4964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_9755_length_4964_cov_6.035241, whole genome shotgun sequence.
ACCESSION   DAOQHY010000214 DAOQHY010000000
VERSION     DAOQHY010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9755_length_4964_cov_6.035241"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..2058)
                     /locus_tag="PLL28_13250"
     CDS             complement(163..2058)
                     /locus_tag="PLL28_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HNF70340.1"
                     /translation="MSYKSLQDFVDVLERAGELVRVKEFVDPKLEITEIADRYIKSDK
                     PALLFENTGTGFPLLINSMGSYKRMCMALGVDHLDAIGDDIAGLFKKFTSPKQGLLDK
                     VMMLPELGKVASWMPKELKGKGACQEVIINDPDLTKLPVMTCWPADGGPFITLPVIHT
                     VDPENGIRNVGMYRMQVFGPKLTGMHWHKHKVSAGHFNKYKQLGKRMPVSVVLGGDPV
                     YTYCATAPLPPNVDEYLLAGFIRKRKVELVKCITNDLYVPADADFVLEGYVDPEEDFI
                     YEGPFGDHTGYYSLADYYPKFHITCITHKKNAIYPSTIVGIPPQEDAWIGKATERIFL
                     TPIKLTMLPEVEDMELPIEGVFHNLTIVKIKKEYAGHAQKVMNAMWGAGQMMFNKILV
                     ICDTSVDIHNYDAVAKAISEHVDPAQDIYFSTGPMDVLDHSCSKFAFGGKMCVDATAK
                     LPEEQTAAHGKKEYPAVDGNVLMQKYPEITHVNDSLLSLGISCLTIALKKSKQGHVRQ
                     LHTLLSAEPGLQHVKFIAYVDHTTEASDNGVVTWNTSNNMDARRDVFITAGTSGNNIC
                     GMDGTRKTKQFDNFDRPWPNVIVMDDKTISTIDEKWAQLGLGPFIVSPSHKYRRQIVG
                     RGAVVEE"
     assembly_gap    2103..2202
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2223..3782)
                     /locus_tag="PLL28_13255"
     CDS             complement(2223..3782)
                     /locus_tag="PLL28_13255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70341.1"
                     /translation="MKRICIASLLLFASLAAIGQTSFSRLYWKYPYVGFGVAVNYDEN
                     YIYIVGRGNSYWDGFLAANRFFIKSDYFGDPISTSYIFRDSIGVWYGGYTSGLEFISD
                     SILVCAGYFSRYDTLFHPWNSQHLGVLYKYNISGDTVLTKKYFGDGYTEFERIVRNDG
                     EITLGGYTSDTTFDGVYGYLIRVEENGLVLNETKCGDGIYDEISGLMDFNGNDRLLAG
                     GVIDETGSDLISINNGIIHKIDNLGNLYFSKEIGTPGNDSEVEVKVAKNGQSYIVRQY
                     IDTVINEGDYEYVQYIGKTDTNVNFIWRTFLNDEFYKYIYTLRTFEDGSIVAVGVTIV
                     DNTYEPHGYIVKLDSNGTVLWERDHTNNPAGNHYLYDFQKMPDGGYICSGMGADTVEG
                     GGIESMCWLLRLDSMGCLIPGCDEVSVPDIAVNSETYLSIYPNPFGDAATVEVHLPQD
                     FNINGNQSLRFDIYDMQGRLIDNYSNIYPSNPGETIRFKMYRNKMTVGNYLATLKYGD
                     EVLNTIQFVIN"
     gene            complement(3787..>4964)
                     /locus_tag="PLL28_13260"
     CDS             complement(3787..>4964)
                     /locus_tag="PLL28_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70342.1"
                     /translation="ISLISGATTATMKKNQGACGTLTSPANNLFTDPSFADINTQIKT
                     SSDITTTNYYKFSFHEDDDAGGSPFAAPAIYSNTIDPLPDGDVFESGCNDVETFTTTN
                     LDDYCSTNFPSGSGGSGRMSAPKATKRRPPTLMMPLTYNPYEYLKPAGVDYEGFLKNI
                     RFNMEASYYLQNELIDSLNILLNEYESPLATSIKVQLLEDLSDYDNAEVLSAELTESP
                     DAELNGYNSSLLSLGLSLSRDTLTWLQTTEEQFSSIKTEAQRNDREGIQAKLLMELIA
                     GGAYIEPINELNEELTTEELRLLQQSMPQSKPNSIQVYPHPVGSGSVVEINLKSIQSN
                     ARFIITDVQGRNVLQFPLDNLVSYVKLDNTLLRPGVYIGYVAGATGEQISKQIVVIQ"
BASE COUNT         1416 a         1096 c          848 g         1504 t
ORIGIN      
        1 gataaaaaaa cacaagtccg ccccaaatga tcaatgtcct gtgcagatca ggaatggtgc
       61 tgaaataatc ttgtatggtg ttgatgaaaa gtaacacgaa tggaaaggat ttaacgggtt
      121 aaaacggatt gtttcctcta aggtaaaaaa tccatccaaa ctttactcct ccactaccgc
      181 tcccctgcca acgatttgtc gtcgatactt atgtgacggc gatacaatga aaggccctaa
      241 gccaagttgt gcccatttct catctatcgt gctgatggtc ttgtcatcca taacgatcac
      301 attcggccat ggtcggtcga aattatcgaa ctgctttgtt ttgcgcgtac cgtccatgcc
      361 acaaatatta ttgcccgatg tgcctgcagt gataaataca tccctgcgcg catccatgtt
      421 gtttgaagta ttccaggtta ccacaccatt gtcggaggcc tctgttgtat gatctacgta
      481 ggcaataaat ttaacatgct gcaaacccgg ttcagctgat aataaggtat gcaattgacg
      541 cacatggcct tgttttgatt tttttagtgc aatggtcaaa caggaaatac ccaatgacaa
      601 taaactatca ttcacatgcg taatctccgg atatttttgc ataagcacgt tcccatcaac
      661 agctggatat tcttttttac catgtgcggc tgtttgttcc tcaggcaatt ttgctgttgc
      721 atccacacac atcttacccc caaaagcgaa tttgctgcag gagtgatcga gcacatccat
      781 tggtcctgtg ctgaaataaa tatcctgtgc aggatctaca tgctcgctga tggcttttgc
      841 tacagcatcg tagttatgta tatcgacaga agtatcgcaa atcaccagaa ttttattaaa
      901 catcatttgt cccgcacccc acatcgcatt catcactttt tgtgcgtgac ctgcatattc
      961 ttttttaatt tttactattg taagattgtg aaaaacacct tcaattggca attccatatc
     1021 ttccacttcc ggaagcatgg tgagtttgat cggcgttaaa aatattcgct ccgttgcttt
     1081 tccaatccat gcatcttcct gtggcggaat gcctacaatg gtagatggat atattgcatt
     1141 ttttttatgc gtgatacaag taatatgaaa tttcggataa taatctgcca atgaataata
     1201 tccggtatga tcaccaaaag ggccttcata aataaaatcc tcttccggat ctacatatcc
     1261 ttccaacaca aaatcggcat cagcaggtac atacaaatca tttgtgatgc attttaccaa
     1321 ttctactttg cgcttgcgga taaatcctgc caataaatat tcatccacgt ttggtggcag
     1381 cggagctgtt gcacaatatg tataaacagg atcaccgccc aataccacac ttacaggcat
     1441 gcgtttgccc agttgtttat atttattgaa atggcctgca cttaccttgt gtttatgcca
     1501 atgcattcca gtaagtttcg gaccaaacac ttgcatgcgg tacataccta cattgcgtat
     1561 tccgttttcc ggatcaacag tatgaataac aggcaatgtg atgaagggcc ccccatcagc
     1621 aggccagcag gtcattaccg ggagtttcgt aagatcagga tcattaataa tcacttcctg
     1681 acaagcacct ttccctttta attctttggg catccaggag gctactttac ctagttcggg
     1741 aagcatcatt actttatcca gcaatccctg ctttggcgaa gtaaatttct taaacagacc
     1801 cgcaatatca tcaccaatag catccaaatg gtcaactccc aaagccatgc acatgcgttt
     1861 ataagagccc atgctattaa tcagcagggg aaaacccgtc cctgtatttt cgaacagcag
     1921 cgcaggctta tctgacttga tatacctgtc ggctatctcc gtgatttcca gtttagggtc
     1981 gacaaactcc ttcacacgaa ccagctcccc tgccctttcc agtacatcca caaaatcctg
     2041 taaactcttg taactcatag cgcaaaggta gtaacaggga gggagaaaaa gtgttcaggg
     2101 ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgaaaata atggaattat
     2221 ggttaattaa ttacaaattg aattgtgttt aaaacttcat ccccatactt tagcgttgcg
     2281 aggtaatttc caactgtcat tttattgcga tacattttaa aacgtatggt ttctccgggg
     2341 ttgcttggat aaatattact gtaattatct atcagtctgc cttgcatatc ataaatatca
     2401 aatctcagtg actggttgcc gtttatatta aaatcctgag gcaaatgaac ttctactgtt
     2461 gctgcatctc caaacgggtt tgggtatata cttagatagg tctcagaatt aacagctata
     2521 tcaggtaccg acacctcatc acaacccgga atgaggcaac ccatgctgtc taaacgtaaa
     2581 agccagcaca tagattctat tcctcctcct tcaactgtat ctgcccccat gccggagcat
     2641 atgtatccgc cgtcaggcat cttttgaaaa tcatatagat aatggttacc ggcaggatta
     2701 tttgtatggt cacgctccca taatacggtt ccgttactgt ctagcttcac tatgtatccg
     2761 tgaggctcgt aggtattatc tacaatggta acgccgactg ctacgatact gccatcttca
     2821 aaagtgcgga gagtataaat gtatttgtaa aactcatcat tcaggaatgt tcgccagatg
     2881 aaatttacat ttgtgtctgt tttacctata tactgtacat attcataatc cccttcgtta
     2941 atcactgtat ctatatactg acgcacaata taagattgcc cattctttgc aaccttaacc
     3001 tcaacttcag agtcatttcc cggcgtgccg atttctttgg agaaatataa attccctaaa
     3061 ttatcaattt tgtgtattat cccgttgtta attgaaatta aatcagatcc tgtttcatcg
     3121 attacaccac ctgccaataa cctgtcattc ccattgaaat ccatcaatcc actaatttca
     3181 tcataaatcc catcaccgca tttagtttca tttaaaacaa gaccattttc ctccactctg
     3241 attaagtaac cgtatacccc atcaaaagta gtgtcgctgg tataaccgcc caaagtaatt
     3301 tcaccatcat ttcttactat cctttcaaat tctgtataac catcgccaaa atatttttta
     3361 gttagaactg tatcacctga tatattatat ttataaagaa ctcctaggtg ttgactattc
     3421 caaggatgga acaatgtgtc ataccttgaa aaataaccag cacaaactaa aatactatca
     3481 gatataaact ccaaaccact ggtataacca ccataccaaa ccccaatgct gtctcgaaat
     3541 atataactgg ttgaaatcgg gtcaccaaaa tagtcagatt ttataaaaaa ccgatttgca
     3601 gcaagaaatc catcccaata gctatttcca cgaccaacaa tataaatata gttctcatca
     3661 taatttacag caactccaaa ccccacatat ggatacttcc aatatagcct tgagaatgaa
     3721 gtttgtccaa ttgccgccaa actagcaaaa agtaacaaac tagcaataca aattcttttc
     3781 atcaaattat tgtataacca ctatttgttt actgatctgt tcacctgttg ctcctgccac
     3841 ataaccgata tacacgccag gtcgcagtag agtgttatct aacttaacat aagaaaccaa
     3901 gttgtcaagc gggaattgca atacatttct gccttgaaca tctgtaataa taaatcgagc
     3961 attactttga attgacttaa gatttatttc aactacactg ccacttccaa caggatgcgg
     4021 ataaacttgt atggaattcg gtttactttg cggcatgctt tgttgaagca acctcaactc
     4081 ctctgtcgtc aactcttcgt ttaattcatt tataggttca atgtaagcgc cacctgcaat
     4141 tagctccata agtaacttcg cttgtatgcc ttctcggtca tttcgctgcg cttcggtttt
     4201 aatggaagaa aattgttctt ctgttgtttg caaccacgtc aatgtatctc ttgataagct
     4261 caaacctagt gacaatagac ttgaattgta tccgttcagt tcagcatcgg gagattcggt
     4321 tagttcggca cttagcactt cggcattatc atagtcactt aaatcctcca ataattgcac
     4381 cttgatgctt gttgccagag gactttcata ttcattcagt aatatgttaa ggctatctat
     4441 gagttcattt tgtaaataat aagaggcttc catattaaaa cgtatatttt tcaaaaaacc
     4501 ttcataatca actccagccg gcttcaagta ttcataagga ttatatgtga gaggcatcat
     4561 cagagttggt ggtcgtctct tcgttgcctt aggtgccgac atgcgtccac tgccgccgct
     4621 tccggatgga aagttggtag agcagtaatc atctagattt gtagttgtga aagtttctac
     4681 atcgttacac ccactttcaa aaacatcacc atcaggaaga ggatcaatag tattcgaata
     4741 gattgcaggt gctgcaaaag gtgaaccacc tgcatcatca tcttcgtgaa aggaaaattt
     4801 ataatagttc gtagttgtta tatcactcga tgttttaatt tgagtgttaa tatctgcaaa
     4861 gcttggatct gtaaataggt tgtttgcagg tgaggtcaat gtcccgcacg ctccctgatt
     4921 ttttttcatc gttgccgttg tagcacctga tatcaatgat atat
//
LOCUS       DAOQHY010000215         4962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_9760_length_4962_cov_5.026289, whole genome shotgun sequence.
ACCESSION   DAOQHY010000215 DAOQHY010000000
VERSION     DAOQHY010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9760_length_4962_cov_5.026289"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2374
                     /locus_tag="PLL28_13265"
     CDS             <1..2374
                     /locus_tag="PLL28_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70343.1"
                     /translation="LEQGTNIFILQLEYPSEWKVKKEPEIILVNEDSIPYLNPAFVHY
                     DRVLAGEPQKFLRNNQASMKVKHPGQVWQYRPALPEDVFTINIPEKKNQFYLARIKVN
                     HDKTIDTFFYHLPKDSSVNICSSGLDTLDDVSDLNKYTDVIKIIQIDLSKPSETRMVS
                     PEPTQFLFKYDYDPAGVNAIGDELSTLRQIRVYDAATLALVQVISTPLALPGSDTELP
                     NRVQAIHLDPNRKSPDIPDLKVVIEEKFDEHFNRSRQRCIFYRYDPLTISYTADSILS
                     QKWDVGFDACKNIIAYDQIIDGNKRHIDTYRFIPGQGWVFSIRKTTYDVPQGGNDIPV
                     TACLSADIPENIMAEVVIRKDKYASGTFVDSIPIYNPCYQKIGLIAEKTEANWEIICP
                     KYLSANDTSWIKIRHAYHASYQISRFNLRFELILQDQSRKVINYSYFNVWDSTMVVNP
                     SNTGSQHFIEIPEKYLLKQPITINSLEIYPDGKPKAYGEVIYGDHQKVGRWEIWDSIS
                     NVAASKWYGEEFIISILNPTHLNSKPLMYAYVGGAKVPIRCFDYYRGTDFKVWLPKDM
                     DSLRIESGKAIFEGSIDKATLSNYSQLYFYLLNPDEQYVRQGYNKIPITWDSDAYVLL
                     WDYADPAYAAAGNNAGKILSARIKARFPDLMLFIAPDGTDAYFPTIMFTNQSPTRRTE
                     ILNFLINDPLIDVVSRKVQYGDEMQTFHFNTITVGINVYQNNQFVQDLADRYGAHLSS
                     IYGSGNLYEFRFSNLVIDESWTKKLEALYHEKGVELVSPSLYSPVVLDGE"
     gene            2637..3272
                     /locus_tag="PLL28_13270"
     CDS             2637..3272
                     /locus_tag="PLL28_13270"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNF70344.1"
                     /translation="MSKTQNTTPDRNTEEAILQAAKRVFTRKGFAAARMEDIATEAGI
                     NRALLHYYFRSKEKMFERVFEENMSHFYGSFISILNAPAPLDMRIRNLVNAEIDMLLA
                     NPDVPLFILTEISRNPDMIKSKMEKMPVREFMMIFVSSLRTEMQKGTIRQIEPVHVLM
                     SLMSLCIFPFIGKPMFLTVSGMPPEQFESLMQSRKKVVADIILQMLKPEQA"
     gene            3269..4525
                     /locus_tag="PLL28_13275"
     CDS             3269..4525
                     /locus_tag="PLL28_13275"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNF70345.1"
                     /translation="MKRFFLIILLLPLTLRAQQSVTLADCQRAAEANYPLLGQSALLQ
                     EITDANIQKINAIWQPQVYVNAQATYQSEVTSLPISFPGIEVPELSKDQYKATIDISQ
                     TLYDGGISSQQKAIAGQNLAVEMQKVEVDKFKIRDKVDQVYMSILMADARIELMVIAV
                     SDLNTVLSISKVGGTNGVVSDQQILILQAEIMKTEQQLTELKALRDGAIRMMAVLTAL
                     PLTSTSVFARPEPAFEGTAMNNQRPELQLYAQQRTLAGLQQQYAEAATLPKVSLFAQG
                     GYGKPGLNFLVDSFSTFYMGGIKVSYPLWTGDTKRRDAKIFGLNSEIIGMQQLQFEQS
                     INVQSAQYQSDIDKYKTLISQDDSLVNLRTSILTMARVQLEQGVISTKDYILYAKDLD
                     DARINRSIHDLQLLATKVNYLNTLGK"
     gene            4532..>4962
                     /locus_tag="PLL28_13280"
     CDS             4532..>4962
                     /locus_tag="PLL28_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HNF70346.1"
                     /translation="MQRIIIIAGLSMLLGACSNGDKIYDATGTFEATEVIVSAGTAGK
                     LLALNVEEGQSLEANAVAAIVDTTQLHLKKAQLNAQIQAVMSREPNIPTQIATIQEQI
                     RVAAGEKQRMEILVKAGAAPSKQLDDIVNQIAVLEKQLDAQR"
BASE COUNT         1467 a         1178 c         1038 g         1279 t
ORIGIN      
        1 cttggagcag gggacaaata tctttatcct gcaacttgaa tacccttccg aatggaaagt
       61 gaaaaaggag ccagagatta ttttggttaa tgaggactca attccttatc tcaaccctgc
      121 atttgttcac tatgatcgtg ttttagctgg cgaacctcaa aagttcttgc gaaacaatca
      181 agcttcaatg aaagtgaagc accccggaca agtgtggcag tatcgaccgg cacttccgga
      241 ggatgttttt acgataaata tccccgaaaa aaagaatcaa ttctatttgg ctagaattaa
      301 ggttaatcac gataaaacga tagacacctt tttttatcac ctcccaaaag actcctctgt
      361 taatatatgt agctcaggtc tcgatacact tgacgatgtt tctgatctga ataaatatac
      421 tgatgtgatc aaaatcatac aaatcgattt gagtaagcct agcgaaactc ggatggttag
      481 ccctgaacct actcaattcc tatttaagta tgattacgat cccgccgggg taaatgcaat
      541 tggtgacgaa ttatcaacgt tgcgacaaat cagggtatac gatgccgcca cccttgcatt
      601 ggtccaagta atctctacac cgcttgcgct gccgggctcc gacactgagt tgccaaatcg
      661 tgttcaggct attcacctgg accccaatcg caaatcaccc gacatacctg atctcaaagt
      721 ggttattgaa gagaaatttg atgaacactt caatagatca cgacagcgct gcatctttta
      781 tagatatgac ccgctcacaa taagttatac tgccgatagc atccttagcc agaaatggga
      841 cgtagggttc gatgcttgta agaatatcat cgcctatgac caaatcatcg atggcaacaa
      901 acggcatatc gatacttata ggtttattcc cggtcaagga tgggtatttt cgattcggaa
      961 aaccacctat gatgttccgc agggtggtaa cgacatcccg gtaactgcat gcctttctgc
     1021 cgacataccg gaaaatatta tggccgaagt agttatccga aaagacaaat acgctagcgg
     1081 cacgtttgta gatagtattc ctatttataa cccatgctat caaaaaattg gtctcatagc
     1141 cgaaaaaacc gaggcaaact gggagatcat ttgtcccaaa tacctctctg ccaatgatac
     1201 ctcgtggatt aaaatcagac atgcttacca tgcatcctat cagatttcgc gattcaatct
     1261 gaggtttgaa ttgatcttac aggatcaatc aagaaaggtg attaattaca gctacttcaa
     1321 tgtgtgggat tctacaatgg tggtcaatcc ttcaaatacc ggatcacaac attttataga
     1381 aatcccggag aaatatttgc tgaaacaacc tatcactata aacagtttgg aaatataccc
     1441 tgatggcaag cccaaggctt atggtgaagt catatacggc gatcatcaaa aggttggcag
     1501 atgggaaatt tgggattcaa tcagtaatgt agctgcttcc aaatggtatg gtgaggaatt
     1561 cataataagc attctcaatc caacccacct caattcaaag ccactgatgt acgcgtatgt
     1621 aggtggggcc aaagtgccaa ttagatgctt tgactactat cgtggcacag atttcaaggt
     1681 ttggctgcct aaggacatgg atagcctaag aattgagagt ggtaaagcta tttttgaagg
     1741 atctatcgat aaagccacct tatcaaatta tagtcaattg tatttctacc tgctcaatcc
     1801 tgatgagcaa tatgtccgcc aaggctataa caagatacca atcacttggg attcggatgc
     1861 ctatgtgctg ttgtgggact atgccgatcc cgcctacgcc gccgcgggaa acaacgccgg
     1921 taagatactg agtgcccgca tcaaagcccg ttttcccgac ctgatgctgt tcattgcccc
     1981 ggatggaaca gatgcttact ttcccaccat catgttcacc aaccaatcgc cgacccgtcg
     2041 aactgagatc ctcaacttcc tcatcaacga cccgctcatc gatgtcgttt cacgcaaggt
     2101 gcaatacggc gatgaaatgc agaccttcca tttcaacacc attaccgtcg gcatcaatgt
     2161 ataccaaaac aatcaattcg tccaggatct cgcagatcga tatggtgcac acctcagcag
     2221 tatttatggc agcggcaacc tatatgaatt ccgattttca aacctggtga tagacgaatc
     2281 ttggaccaag aaactggagg cactctacca cgaaaaaggg gtagaactcg tttctccttc
     2341 gctgtattct cctgtggttc ttgatggtga ataagcctca ttttaaccgc ttcaatcttc
     2401 cggcagctta atggcatttt accactttga caagttgaca ggcctcgtat tttgcaagaa
     2461 tttcaattat tttttgttat gttttttata ttttaacatc tgattatcaa ttacttatat
     2521 ttaattatat aatattatat ttgagataac gcaaaaaata tattttcctc ggaatttgga
     2581 gatttgaaag cggtacttat acatttgcat cattgtttaa caattttgtt aaaccaatga
     2641 gtaaaacgca aaatacaacc cccgaccgca atacagagga agccatcctg caggctgcga
     2701 agcgtgtatt cacccgcaag ggttttgctg cagcacgtat ggaagatata gccactgagg
     2761 caggcatcaa tcgcgccttg ttgcactatt acttccggag taaagagaaa atgtttgaac
     2821 gggtgttcga ggagaacatg tcgcatttct atggcagctt tatcagcatc ctgaatgcgc
     2881 ctgccccact cgacatgcgc atccgcaacc tggtgaatgc cgagatcgat atgctgctgg
     2941 ccaatcccga tgtgcctcta ttcatcctca ctgagatctc acgcaacccc gacatgataa
     3001 aatcaaaaat ggaaaaaatg ccggtccgtg aattcatgat gatcttcgta tccagcctcc
     3061 ggactgaaat gcaaaaaggc accattcggc agatcgaacc tgtacatgtg ctcatgtctc
     3121 tgatgtccct ttgcatcttc ccatttatcg gcaaacccat gtttctcacc gtaagtggta
     3181 tgccccccga acagttcgag tcactcatgc agtcacgtaa gaaagtcgtg gctgatatca
     3241 tattacaaat gctcaaacct gaacaagcat gaaacgcttt ttcctgatca tactccttct
     3301 ccctttaacg ctaagggcac agcaatcagt cacgcttgcc gactgccaac gcgctgccga
     3361 agccaattat ccactgcttg gtcaatctgc actattacaa gagataactg atgctaatat
     3421 acagaaaatc aatgcaatat ggcagccaca agtttatgtt aatgcccagg caacttacca
     3481 aagtgaggta accagcctgc ccatttcttt ccccggaatc gaagtaccgg aattaagcaa
     3541 agatcagtat aaggctacta tcgatatcag ccaaacactg tatgatggag gtatctcctc
     3601 ccagcaaaaa gctatcgccg gacagaacct ggcagtggaa atgcagaagg tagaggtaga
     3661 caaattcaag atacgcgata aggtagatca ggtgtacatg tcgatactca tggctgacgc
     3721 aagaattgag ttgatggtaa tagcggtgag cgatttgaat acggtccttt ccatcagcaa
     3781 ggttggcggt acgaatgggg tggtcagcga ccagcagatt ctcatcctgc aagccgagat
     3841 catgaaaacg gagcaacaac ttacagagct gaaagcactt cgtgatggtg ccatccgtat
     3901 gatggccgta ctcactgccc tgcccctcac ttcgacttca gtattcgcaa gacctgagcc
     3961 tgcttttgaa ggcaccgcta tgaacaatca acgccccgaa ttgcaacttt atgctcagca
     4021 acgcaccctt gccggactac aacaacaata tgccgaagca gccaccttgc ccaaagtaag
     4081 cctcttcgca cagggcggtt atggcaagcc gggactcaat tttttggtgg atagtttcag
     4141 tactttctac atgggcggta tcaaggtttc ttaccctttg tggacaggcg atacgaagcg
     4201 ccgcgatgcg aaaatctttg gcttaaacag tgagattatc ggcatgcagc agctacaatt
     4261 tgaacagagt ataaacgtgc aatctgctca atatcagtca gatatagata aatataaaac
     4321 cctgatatca caagatgaca gcctggtaaa tctgcgcaca tctattctaa cgatggcacg
     4381 tgtacaactg gaacagggtg ttatctcaac caaagattat atcctttatg ccaaggatct
     4441 ggacgatgcc cgtatcaacc gatcgatcca cgacctacag cttctcgcaa caaaagtgaa
     4501 ctatctgaat acactcggaa aataacctat catgcaaaga atcatcataa tcgcaggcct
     4561 aagtatgctt ctcggagcct gttcaaatgg cgacaaaata tatgacgcca caggcacctt
     4621 tgaagccacg gaagtgattg taagcgccgg aactgccggt aagttactcg ccctcaatgt
     4681 tgaagagggg cagtcgctcg aagccaatgc tgtggctgct atagtcgata ccacccagct
     4741 tcacctcaaa aaggctcaac tgaatgcaca aattcaggct gtaatgagtc gcgaaccgaa
     4801 cattcctaca caaatcgcta ctattcagga gcaaattcgg gttgctgccg gtgaaaaaca
     4861 acgcatggaa attctcgtaa aagcgggagc cgctccttct aaacagctcg atgacatcgt
     4921 gaatcagatc gcggtgctgg agaagcaact cgatgcgcag cg
//
LOCUS       DAOQHY010000216         4929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_9862_length_4929_cov_6.860689, whole genome shotgun sequence.
ACCESSION   DAOQHY010000216 DAOQHY010000000
VERSION     DAOQHY010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9862_length_4929_cov_6.860689"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PLL28_13285"
     CDS             <1..292
                     /locus_tag="PLL28_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNF70347.1"
                     /translation="TGSKMDEVIFEEFKGTGNMELQLDRKLFNRRIFPSIDIINSSTR
                     RDDLLHEREVLQRMWVLRNYLADMNTEEAMQFLLQRMRGTKSNEEFLATMNG"
     gene            complement(573..1220)
                     /locus_tag="PLL28_13290"
     CDS             complement(573..1220)
                     /locus_tag="PLL28_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70348.1"
                     /translation="MRVKLLSILFCFIAVFCYQNRLSAQLVEFGIGYTPSIFSGSEIS
                     SFDGGDVPTVNTVSSVENTLVLSGNMMVGIYYHLLHYGENMSLGAHYQATLFAGTMDG
                     GYYDALMGGVDLPIMAEIRFGNIATESSTAPFGCQLGGGAMFTAGIISIDGHPLKLGM
                     FKPAVSLGATYEDVGIQFLWHPGKYTILYDTNTGGIPRADFFKYHLTLFWYLGEL"
     gene            complement(1211..2188)
                     /locus_tag="PLL28_13295"
     CDS             complement(1211..2188)
                     /locus_tag="PLL28_13295"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HNF70349.1"
                     /translation="MIRSIQILVLSVIIAGCNSEKIFIPTVRFEEVFFGDAPLNEVYF
                     TSATTGVIVGDEGQFLQSTDGGRTWLQVAEAPIDINYNVISFPTTSIGYVSGDGALLK
                     TVDGGVTWNLIFEGYHFIYTCFPTATIGYAIDGNYVYKSTDGGETWNDIYAYSSCGIA
                     QARAIYFYTASSGYLVDESWSYVATSTSGGNSFTYAIERPSIAYAHTRRAVNGLSYWA
                     AGNDTYYSGMYYTTDGDPIERFSTDGFATENSSYIYAMHSWDGNEFIAIGTSTIALSH
                     DSGETWTEVFDQNGFNLRLHDAAVLENGRYVGIYDRVVYLLTQDCTTCE"
     gene            complement(2475..4664)
                     /locus_tag="PLL28_13300"
     CDS             complement(2475..4664)
                     /locus_tag="PLL28_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNF70350.1"
                     /translation="MSSQVLLITPPFTQLNTPYPATVYLKGFLNTLGISSRQADLGIE
                     TTLKLFTRRHLQKVFDLAEEHINNDDRHYQLWEQRDTYIDVAESVVAFLQGKLPALAH
                     QIVSGNMLPVGERIAQVDKSDLAYQQLDLQDKAKHLATLYLEEIADFIRDTIDPYFGF
                     TRYAERLARSANSFDALYEALNQDYTYVDTLMCELLDEHIHHSQPTLIAITAPFPGNV
                     YAAFRCAQHIKNNYPNISIAFGGGFVNTELRSLKDVRVFEFFDFISLDDGEAPIECLW
                     KYIQGEISVDNLKRTFILQSGAVRYVNNASCVDYAFADTGTPDYSDLPLDKYIHAIEI
                     INPMHNLWSNGRWNKLTMAHGCYWGKCTFCDVSLPYIADYSPLSAGMIVDRMESMIAQ
                     TGETGFHFVDEAAPPALMREVAVEILRRQLNVTWWTNIRFEKSFTRDLCVLLMASGCI
                     GVSGGLEVASDRLLALIKKGVTVEQVAIVLRNFTDAGIMVHAYLMYGFPSQTDQETID
                     SLEIVRQLFDADVLQSAFWHRFAMTAHSPVGLQPEVFNVNAHRAHNIAFADNDVEFTD
                     ITGGDHEKFAEGLRISLYNYMQGAGLDLPLQKWFNHGIPKTTITPDHINRILDRNEIN
                     IPKATQRVIFLGLLLSASTRTKSKRGNIYTETTLQFQFEENICELKLPEPHASWFQSI
                     LPMLHPAQPQQTISALQNLYAQQGLEDFELFWVNELSGLYEVGLLVI"
     gene            complement(4706..>4929)
                     /locus_tag="PLL28_13305"
     CDS             complement(4706..>4929)
                     /locus_tag="PLL28_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70351.1"
                     /translation="QTYEMLHLHLYSLHYYKRAATLRPSDARMWCAVGNCLSRLASGG
                     IHSRSHELGLNAKREAIEAYKRAVACEDR"
BASE COUNT         1447 a         1011 c         1062 g         1409 t
ORIGIN      
        1 aacaggctca aaaatggatg aggtgatttt tgaggaattt aaaggaacgg gcaatatgga
       61 gttgcagttg gatagaaaat tattcaacag aagaatattc ccttcaatag atatcatcaa
      121 cagttctaca cgccgcgatg atttgttgca cgaaagagaa gtgttgcaac gtatgtgggt
      181 attgcgcaat tatctagctg atatgaacac ggaggaagca atgcaatttt tgttgcaacg
      241 catgagagga acgaagagca atgaggagtt tttggctacg atgaatgggt gaggttaatt
      301 agccaattag cgtattagcc gattagccaa tggggggcgt gcttgcatgg agattttcgt
      361 tttagtgagc aagtttgagg gttagcaagt tagcaggtga gggtaattag caaatttgag
      421 aattagcaaa ttagcaagtg gtggtgcttt taacgctggc gcgttaatag ggaatgcaat
      481 tatattgctt tgcaatattt ttattccgaa gcgccattat ctcccctctc tatcgacggt
      541 aggagatgga gaggggccgg gggtgaggcg ggctataatt cgccgaggta ccagaataag
      601 gtaagatggt atttaaagaa atctgcgcgt gggattccac cggtgttggt gtcgtagagg
      661 atggtgtatt taccggggtg ccacaggaat tggataccta catcttcgta tgttgctcct
      721 aatgaaactg cgggtttgaa cataccaagt ttaagagggt ggccgtctat tgaaattatt
      781 cccgcggtaa acatggcgcc accacctaat tgacaaccaa acggggcggt gcttgattct
      841 gttgcaatat tcccaaatcg gatttcggcc attattggga ggtctacgcc gcccattaaa
      901 gcatcgtaat aaccgccatc catggttccg gcgaaaagag ttgcctggta gtgtgcgcca
      961 aggctcatat tttcaccgta atgcaataga tggtaataaa taccaaccat catatttcca
     1021 cttaaaacta gtgtattctc aacactgctt accgtattaa ccgtaggcac atctccacca
     1081 tcaaaagagc ttatttcact tccgctaaat atggatggcg tataaccaat accgaattct
     1141 actaattgag cagatagccg gttttggtaa caaaaaacgg caataaaaca gaataatata
     1201 gacagcaatt ttactcgcat gttgtacaat cttgagtgag gagatataca actctatcat
     1261 aaatacctac atatcttcca ttttccaaaa ctgcggcatc gtgtagacga agattaaagc
     1321 cattttgatc gaacacttcg gtccatgttt ctccactatc atgtgaaagt gcaattgttg
     1381 acgttccaat agctataaat tcattcccgt cccaagagtg catggcatat atgtaacttg
     1441 agttttctgt cgcaaatcca tctgttgaaa atctttcgat cggatcgcca tcggttgtat
     1501 aatacatacc ggaataataa gtatcgttcc ccgcagccca gtagctcaaa ccatttacgg
     1561 ctctgcgtgt atgtgcgtaa gcgatggagg gccgttcaat tgcgtaggta aagctatttc
     1621 caccactggt gctggtggct acatagctcc agctttcatc aacaagatat cccgaacttg
     1681 cagtatagaa ataaatagca cgagcctggg caattccgca gcttgaatag gcataaatgt
     1741 cattccatgt ttctccgcca tcagttgatt tatatacata attgccatca attgcataac
     1801 cgattgttgc ggtaggaaag caagtataaa tgaaatgata tccttcaaaa ataagattcc
     1861 aggtaacgcc gccgtctaca gtttttaaca aggctccatc gccactcaca taacctatcg
     1921 aagttgtcgg gaagctaatg acattgtaat ttatatctat cggagcttcg gcaacctgca
     1981 gccacgttct accgccatcg gtcgattgta aaaactgacc ctcatctcca acaataactc
     2041 cggtagtagc acttgtaaaa taaacttcat taagcggcgc atcaccaaaa aaaacttctt
     2101 caaaccgtac ggtgggaata aatatttttt ccgaattgca gcccgcaata atgacagata
     2161 agaccaatat ttgtatgctg cgaatcatgt ggttttctaa gtgtgaaata aagatagggg
     2221 aaatatgttg gaaattgtgt tcgggtgttc gtgtgcgggc tgatttgtgg aatgtgctta
     2281 agccggcaat ccgtgtaatc cttcaatccc aataatccgt gattcagaca ataattgtgc
     2341 tcatgtgttc gtgtgcgggc tgatttgtgg aatgtgctta agccggcaat ccgtgtaatc
     2401 cttcaatccc aataatccgt gattcagaca ataattgtgc tccgaattga actaataaat
     2461 ttattcccgg ttgcctaaat caccaataac ccaacctcat acaaaccgct cagctcattc
     2521 acccaaaaca attcaaaatc ttccaaccct tgttgagcat acaaattttg gagagctgat
     2581 attgtttgct gaggttgtgc cggatgtagc attggtagta tagattgaaa ccatgaagcg
     2641 tgtggctccg gtaatttcag ctcgcatata ttttcttcga attgaaattg aagtgtggtt
     2701 tcggtataaa tgtttcctct ttttgattta gtgcgcgtgg atgcgctgag taataatccc
     2761 aggaaaataa cgcgctgcgt tgcttttgga atatttattt catttctgtc taggatgcga
     2821 tttatgtggt cgggtgtaat tgtggttttt gggataccgt gattaaacca tttttgtaaa
     2881 ggaagatcaa gtccggcacc ctgcatgtaa ttgtatagcg atatgcgcaa tccttcagca
     2941 aatttttcgt gatcgccacc ggtgatatcg gtaaattcta catcattatc ggcaaatgca
     3001 atattgtgtg cccgatgtgc atttacgtta aaaacttccg gctgcaatcc aacggggcta
     3061 tgtgctgtca tggcaaagcg atgccaaaag gctgattgca aaacgtcggc atcaaaaagc
     3121 tgacgcacaa tttccagact gtcgatcgtt tcctgatctg tctgtgatgg aaagccatac
     3181 attaaatatg catgcaccat aataccggca tcagtaaaat tgcgcagcac aattgcaact
     3241 tgttcaactg taacgccttt ttttattaaa gcaagtaatc tgtcagatgc aacttctaaa
     3301 ccaccactta ctccaataca tccactggcc atcagcaata cacagaggtc gcgggtgaag
     3361 cttttttcaa atcggatgtt agtccaccag gtaacattta attgcctgcg caaaatttca
     3421 acagccactt cgcgcatcaa cgccggcgga gcggcctcat caacaaaatg aaatccggtt
     3481 tcacccgttt gtgctatcat ggattccatg cgatctacta tcatgcctgc ggagagcgga
     3541 ctgtaatctg caatataggg caacgacaca tcacagaaag tgcatttacc ccaataacaa
     3601 ccatgtgcca tggtgagttt attccatcgt ccgttgctcc acaggttgtg catgggattt
     3661 attatttcta tcgcatggat atatttatcc aaaggcagat cggaataatc aggagttccg
     3721 gtatcggcaa aagcataatc gacgcatgat gcattgttga cgtaacgcac ggcaccgctt
     3781 tgtaaaataa atgtgcgttt taagttatcg actgatattt caccttgaat atatttccac
     3841 aaacattcaa ttggtgcttc gccatcgtct aaagaaataa aatcaaaaaa ttcaaacaca
     3901 cgcacatctt taaggctgcg taattcggtg ttcacaaagc caccgccaaa tgcaattgaa
     3961 atgtttggat aattattttt gatgtgttga gcacatctaa aagccgcata tacatttccc
     4021 ggaaacggcg ctgttatggc aatgagtgtt ggttgggagt gatgaatgtg ttcatcgagc
     4081 aactcacaca tcaacgtatc aacataagta tagtcttgat ttagcgcctc gtataatgca
     4141 tcaaatgaat ttgccgatct cgccaggcgc tcggcataac gtgtaaatcc gaaatacgga
     4201 tctatggtat cgcgaatgaa atctgcaatt tcttctaaat ataaagttgc gagatgcttt
     4261 gccttatctt gtaaatctaa ttgttgatat gctaaatcag acttatccac ttgtgcaatt
     4321 ctttcgccaa ctggaagcat attgccactt acaatttggt gtgccaatgc cggcaatttt
     4381 ccttgaagaa atgcaacaac cgattcggct acatcaatat atgtatcgcg ctgctcccat
     4441 agttggtagt ggcgatcatc attattgata tgctcttctg ctaaatcaaa aactttttgc
     4501 aggtgtctgc gggtaaataa tttcaatgtc gtttcaatgc caagatctgc ttgtcgcgat
     4561 gaaataccaa gcgtatttaa aaaacccttc aggtacaccg tggccggata aggcgtattg
     4621 agttgggtga aaggaggggt gatgagcaga acctgggagg acatgggtaa aaataagaag
     4681 aaagaagaaa ggagaagtaa tagtattacc tgtcttcaca agcaactgct cgtttgtaag
     4741 cttcaattgc ttctcgtttt gcatttaaac ccagttcatg tgaacgactg tgaattccac
     4801 cagaagccaa tcgagacaaa caatttccaa ccgcacacca cattcttgca tcacttggac
     4861 gaagagttgc agctcgttta taataatgaa gtgaatacaa atgaagatga agcatttcat
     4921 aagtttgac
//
LOCUS       DAOQHY010000217         4906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_9921_length_4906_cov_5.270047, whole genome shotgun sequence.
ACCESSION   DAOQHY010000217 DAOQHY010000000
VERSION     DAOQHY010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9921_length_4906_cov_5.270047"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PLL28_13310"
     CDS             <1..252
                     /locus_tag="PLL28_13310"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNF70352.1"
                     /translation="VDIIARKGNILVIVEVKTRSGDPDIDPGDSITRKKINLLAGAAE
                     AFMLDEGLMLLLRYDAILVEKQGIHWEITHIEDAFYPAE"
     gene            249..635
                     /locus_tag="PLL28_13315"
     CDS             249..635
                     /locus_tag="PLL28_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNF70353.1"
                     /translation="MITGVHHIAIICSDYHRSKKFYTEVLGFSILAEHYRAERDSYKL
                     DLRVDENTAIELFSFPGPPARPSYPEAAGLRHVCFKVKDLDAAIAHLQGFQVKCEDVR
                     IDAYTGMRFTFFADPDGLPIELYEDK"
     gene            complement(677..1195)
                     /locus_tag="PLL28_13320"
     CDS             complement(677..1195)
                     /locus_tag="PLL28_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNF70354.1"
                     /translation="MTSSEFQQEIVQLKAPMKAFALNLTRDRDDALDLVQETYMRALS
                     NQEKFHDGTNLKAWLLTIMKNIFINNYRKASKRNTVADASDNLYLLNAGVATAGNEAE
                     KDFVMKDLLKAIQQLDAEYKRPFIMHYHGYKYEEIADAMKLPLGTVKSRIFFARKQMK
                     DFLDKRGFERFQ"
     gene            complement(1282..2181)
                     /locus_tag="PLL28_13325"
     CDS             complement(1282..2181)
                     /locus_tag="PLL28_13325"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNF70355.1"
                     /translation="MSAIYSIRDLESLSGIKAHTIRIWEQRYGILKAARTETNIRYYS
                     ETELKYLMSLALLNRNGFKISKLAIYSPDEIAEKVEHLINEPQEFHPQIEALTLATMA
                     YDEEKFDKVLNTTILQMGLEAAMRKVIFPYLDKLGMLWVSGSVIAAQEHFMTQLLRQK
                     LIVAIDGQIPHYTGDSLTYVLFLPNGEWHELGLLFLHYLLKSRNHRVIYLGPSVPLQD
                     VLQVGESLNPNAFYTVLTTNPNGFHVVDYLNKLAEAFPCSTVFSSGSQVAGPLKGLAS
                     NVKIIDSIDSIVDSPEVLMAENR"
     gene            2891..3745
                     /locus_tag="PLL28_13330"
     CDS             2891..3745
                     /locus_tag="PLL28_13330"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNF70356.1"
                     /translation="MKHILIPSDLRIESDPVWQYVLPLVKTMGASLHFVHAVDDPFVK
                     DERDHVGAEGVDKITSDLVYKYRDEADKAMIKLLDRVKAAMTGMDIHVEGHVRNGNAE
                     EVILHEAAEIQPQLIVMGSHDHSKLDRMFFGSVTQAIIRRSHFPVLAIPEKYIFKPVN
                     QIVYMSDLDNNDTISIAKLLNLFGGNAFNLHITHFNLDEVSKDEGLYAIGDKIRNDHK
                     EIQIDFEVVDARVLRDAYKQYISMKNIDLIALTTRKDHGMMRFINPGTAIDVLYHSKL
                     PVLIFHRA"
     gene            complement(3748..4509)
                     /locus_tag="PLL28_13335"
     CDS             complement(3748..4509)
                     /locus_tag="PLL28_13335"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70357.1"
                     /translation="MKHCLVAIFVVLAGVVSAQTTAENWTQTDCDGVDHDLFTTLDTG
                     AAVVMEFVMLESCMPCINAAHMMAPIIDRYDLLYNNRVQYYTFGYNDTYLCDDFATWE
                     SDNAIANDAALLLGADISSYYGGMGMPTIVVVGRNTHQVYFNQFGFVIGDTTDFSNAI
                     AYALGIADPETGVESMDDEQLLISPNPAFNSIQINGILEPNATLRIINLQGQICMDGI
                     NPTENPIDIQMLKPGMYLLECKTDTGISIAKFTVQ"
BASE COUNT         1448 a          958 c         1084 g         1416 t
ORIGIN      
        1 gtcgatatta ttgccagaaa ggggaatatc ctggttatcg tggaagtgaa aacacgaagc
       61 ggtgatcctg atatagatcc gggagatagc atcaccagaa aaaaaataaa tttgcttgcc
      121 ggggctgcag aagcttttat gttagatgag ggattaatgc tattactgcg ttacgatgcc
      181 atattagttg agaagcaagg catacattgg gagatcacac atattgagga cgcattttat
      241 cctgcggaat gataacagga gtacatcata ttgccattat ttgttccgac tatcataggt
      301 cgaaaaaatt ctatactgaa gtattgggat tttcgatttt agccgaacat tatcgtgctg
      361 aacgcgatag ttataagttg gatttgaggg tagatgaaaa tacggctatc gaattatttt
      421 catttccggg tccgcctgct cgtccgtcgt atccggaagc tgccggactg agacatgtat
      481 gttttaaagt taaagatctt gacgctgcaa tagcccatct gcaaggcttt caggtgaaat
      541 gtgaggatgt gcgcattgat gcctatacag gcatgcgatt tacatttttc gccgatcctg
      601 atggattgcc tattgaatta tatgaagaca aatgattgaa tagaaaagtc aatccttaat
      661 taaacgcatg atcgatttat tgaaatcttt caaacccgcg tttatccaga aaatctttca
      721 tttgttttct tgcgaagaag atgcggcttt tcacggtgcc taaaggcaat ttcatggcat
      781 ctgcaatttc ttcgtattta tatccatgat aatgcatgat gaatgggcgc ttatattcgg
      841 catctaattg ttgaatagcc ttgagtagat ccttcatcac aaaatctttc tctgcttcat
      901 tacctgcagt ggccacacct gcatttaaca gataaagatt atcgctggca tcggcaacag
      961 tgttcctttt cgatgctttg cggtaattgt tgatgaagat atttttcatg atagtgagca
     1021 accaggcttt gaggttggtt ccatcatgga atttctcttg attcgagagc gcacgcatgt
     1081 aagtttcctg aacaagatcg agcgcatcat ctctatcgcg ggtcaggttc agggcaaagg
     1141 ccttcattgg cgcttttagc tgaacgatct cttgttgaaa ttctgagctt gtcatggtcg
     1201 ttgtttgaag caaatttacg gctcaaaaat gccgtttcca aatttttgtt taacttttga
     1261 tgttaaatct tcaacaaaat attaacgatt ttccgccatc agcacttcgg gactatcaac
     1321 gatactatct atagaatcga tgattttaac gttggaagcc aatcctttca agggtcccgc
     1381 aacctgagag ccacttgaaa atacggtgct gcaaggaaat gcttcagcca gcttattcag
     1441 ataatctacg acatgaaagc cgtttgggtt tgttgtgagg acggtataaa aggcattggg
     1501 gttcagcgac tcgccaacct gcaggacatc ctggaggggt acgctgggtc cgagatatat
     1561 tacacgatgg tttcttgact tgagcagata atgcaagaaa agcaatccca attcatgcca
     1621 ttcgccgttc gggagaaaca gcacataggt aagtgaatcg ccggtataat gcggtatttg
     1681 tccatcgatg gcgacaatca gtttttgtcg cagtaattgc gtcataaagt gttcctgcgc
     1741 cgcaattacg gagccagaaa cccagagcat gcccagttta tcgagataag ggaaaatcac
     1801 cttacgcatg gcagcttcaa gacccatttg caggatggtg gtattgagaa ctttatcaaa
     1861 tttctcttca tcataagcca tggtagcaag cgtaagcgct tctatctgag gatggaattc
     1921 ctgtggctca ttgatgaggt gttctacttt ttcggcaatc tcatcaggcg aataaatggc
     1981 cagtttagat atcttgaatc cattcctgtt gagcaaagcc aggctcatca gatactttag
     2041 ctccgtttcg ctgtaatagc gaatattcgt ttcggtacga gccgctttca atatgccata
     2101 acgctgctcc caaatcctga tggtatgtgc ttttataccc gaaagcgact ccagatcgcg
     2161 tatggaatag atggccgaca tgaggtaaaa ttaatgttta agttttgtat ttcaatggtt
     2221 aaacaattat ggagactata ggttcattat ggtggggttg aaggttgaag gaagaatcga
     2281 agatttgaag attcgaagat ttgaaggtgg aaagaatcga aggacgaaga accaaagatt
     2341 cgaaggacga agaatcgaag attcgaagga agaagaaccg aagattcgaa ggacgaagaa
     2401 ccgaagattt gaaggaagaa atgaagggag aagcagtcaa aaactcacca ctcaccactc
     2461 accattcacc cactgcccca ctcacccact gacccactac caatcgtgac tcgtgaatcg
     2521 tgaatcgtga gtggtgagtt tatcctgata cctgatacct gatacttgat acttgatact
     2581 tgatacttga tacttgatac ctgatacctg atacttgata cctgatacct gatacttgat
     2641 acctgatacc tgatacctga tacttgatac ctgataccaa gtcttgatac tagatacccg
     2701 ccggaggcga ggcgagcttg gctctagata cttaattcta gatactaata cctccagtcg
     2761 gttgcagact gaaatctaga tagcagaggc gtgatagtca gatgaaggtt tacgggatga
     2821 ttaagaatgg tgacttattt cattcttttt gaaatgacct gcctttatct ttaccatcaa
     2881 aaccactaat atgaaacata tactgattcc gagcgacttg cgcattgaat ccgatcctgt
     2941 ttggcaatat gtattaccac tggtcaaaac catgggtgcc tcccttcatt ttgtgcatgc
     3001 tgttgacgat ccatttgtaa aagatgaacg cgatcatgtt ggagctgagg gtgttgataa
     3061 aattaccagc gacttggtat ataaatatcg cgatgaagca gataaggcga tgataaagtt
     3121 attggaccgc gttaaggctg ccatgacagg aatggacatt cacgttgaag gtcatgtacg
     3181 taatggcaat gccgaagagg tgatactgca tgaagctgcg gaaatacaac ctcaactcat
     3241 tgtaatggga agtcacgacc acagcaaact ggatcgcatg ttttttggaa gtgtgacaca
     3301 ggcaattatt cgtcgctcac attttccggt acttgctatt cctgagaaat atatattcaa
     3361 gccggtaaat cagatagtct acatgagcga tctagacaat aacgatacta tatctatagc
     3421 caagttgctc aatttattcg gcggaaatgc attcaacctg catatcaccc atttcaatct
     3481 tgatgaagta tccaaagacg aaggattata tgccattggc gacaagatcc gcaatgatca
     3541 caaagaaata cagatagatt ttgaagtggt agatgcgagg gtattgcgcg atgcttataa
     3601 gcaatatata tccatgaaaa atatcgatct aatcgccctt acaacacgca aagatcatgg
     3661 tatgatgcga tttataaatc cgggaactgc tattgatgta ttatatcaca gcaaattgcc
     3721 tgtgttgata tttcacagag cttaaattta ttgcactgta aacttcgcta tagaaatgcc
     3781 tgtgtccgtt ttgcattcaa gaagatacat gccgggctta agcatttgaa tgtcgatggg
     3841 attttcagtc ggattaattc catccatgca aatttgacct tgcagattta ttatgcgcaa
     3901 tgttgcatta ggctcaagaa ttccatttat ttgaatgcta ttaaaagcag gattaggtga
     3961 tatcaataat tgctcatcat ccatactttc aacgcctgtt tcaggatctg ctatcccgag
     4021 tgcatacgct atagcattag aaaaatcagt agtatcgccg atcacaaatc cgaactgatt
     4081 aaaataaact tgatgggtat ttcttcccac cacgactatg gtgggcattc ccattccgcc
     4141 gtaataactg ctgatatcgg cacccaataa gagagctgca tcatttgcaa tagcattatc
     4201 actttcccat gttgcaaaat cgtcgcacaa ataggtgtcg ttatatccga aggtataata
     4261 ttgtactcgg ttgttgtaaa gtaaatcgta tctatcaata atcggcgcca tcatatgtgc
     4321 agcattgatg cagggcatac aactctcaag catcacaaat tccatcacca ctgccgcacc
     4381 ggtatcgagt gtagtaaaca ggtcatggtc aacaccgtcg caatcagtct gtgtccagtt
     4441 ttcagcggtt gtctgcgccg aaacaacacc ggccaaaacc acaaaaattg ctacaagaca
     4501 atgtttcatg agataaagat aggaaatagt tcgtgattgg gtcttttctc aggggattga
     4561 ataaatgcta ttcgaatgaa aaggagggtt aattagcaaa ttagcaaatt agcaagttag
     4621 caagtgttat gggcttttaa cgctggcgcg ttaataggga atgcgtttta cattgtttaa
     4681 taaaatttta ttccgaagcg aatttagctc cctctctatc gacgatagga gatggagagg
     4741 gcggggggtg aggcgagaat tagcaaatta gcaagtgagc aagtgagcaa attagcaagt
     4801 tagcaagtgt ggggtgtttt taacgctggc gcgttaatag ggaatgcaat gatattgctt
     4861 tgcaatattt taattccgaa gcgtaattag ctccctctct atcgac
//
LOCUS       DAOQHY010000218         4877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_10007_length_4877_cov_5.481958, whole genome shotgun sequence.
ACCESSION   DAOQHY010000218 DAOQHY010000000
VERSION     DAOQHY010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10007_length_4877_cov_5.481958"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="PLL28_13340"
     CDS             <1..138
                     /locus_tag="PLL28_13340"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HNF70358.1"
                     /translation="FSHKEISEQLNISEGTSKSQLARARYILMEMVEQRTGLKRKAIG
                     E"
     gene            128..1363
                     /locus_tag="PLL28_13345"
     CDS             128..1363
                     /locus_tag="PLL28_13345"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNF70359.1"
                     /translation="MENNNHHIDRIFKNGLKHYSAQPSADLWNRIADELDNGMPAKSS
                     RAQWYRYAAMFLVLLSVALISVPGNRHTPPAIFAFDNNTGNSYAAPISHTAMPSFTAL
                     IPEITQVVADNISLRRTPSESVSFENPVQQITTADLSIKTHPKDAFIYLPAEPENETL
                     AAASQDVFDRFYNNDEPIDVMAYNYFGDDDPEADFFEAPSYKAKYRDYDMRGLYIGAT
                     GSYNQTSLLESGNVFKGERPIQPSLKFGTTKGLRVGYNINNSFGIEAEYVYNALQGQN
                     YVMSEGDDIVEKSLSLNYDLIPVVAKVKIGHISDITNKPVVLNYTAGVQYGMLREARM
                     PQDKRYEESAMELFKETDVSVVLGLEYDVFVQDNIVISAGARGTFSNDISTHIEPLND
                     YAKRNFVFGLHGGVSYVFR"
     gene            1402..3861
                     /locus_tag="PLL28_13350"
     CDS             1402..3861
                     /locus_tag="PLL28_13350"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 and carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNF70360.1"
                     /translation="MRKCLIYVCLLIILITRLSAGTISGHITDTTGAPLPFCNVYLSG
                     TTYAVPANAEGFYQLTAPNGEYVIVFQFIGYSRKEIPITISDKPLMFDVVLQEEMTEL
                     QTIVIGPDHEDPAYAVIKQAIERRADHLKEVNAYSCDVYMKGLQKLNEAPDQIMGIKL
                     NTVFDVDSNNTGIIYLSESVSSFNYKYPDQTKEIMKASVVSGDKNQFSWNDAAGMQMN
                     FYQNLETLQGFSQRGFVSPIADNALFFYTYHLLGNTFENNREIFKIQVTPKRSGDPAY
                     TGVIYITNDDYRITGLDLTLTKQNGIEFLDTFNVTQEYYYADREHLMLLSNKFSFNYG
                     MFGIQGEGYFHAYYKNYTINPIFDKHFFDGEVIRINAGANKQDSTYWAGIRPMQLTKE
                     EQLDYHEKDSLAILKESPAYQDSMDHIFNKFSPAVLITGYNWRDSKSKSMISTNNIFD
                     LLQFNTVENYVIQPRLTYTHWYEDNRRFEIGTTLRWNTATNGALISMQSRYLLDPVSQ
                     EKLSLLAGSRMQQFNPMGISPLVNSLYTLLLEQNSMKLYAEDFIQFDADREMYNGFYF
                     GIGAYYGKRYRQYDAINTEPWIDDQSKYFSNNNDFVIHNATVFEWGPMSDILMLHLHI
                     KYVIKQTYILHPDEKFINEPKYPVIELDLRSANVTLNDELDQFNKIHLSVFDRLPLSL
                     AGNLEYIGTATVQFADYEQLIVDKIHAIGNETNVSRLQNETFYLLPYYYATSSDYMFT
                     GHVSWHTEGFLFRKLPLFKQLKMEPVFSAHVLTSDTMPFYTEFTFGVEHLFKIIRVDL
                     AMTPMHDDFIPPARLFIGFGF"
     gene            3864..4808
                     /locus_tag="PLL28_13355"
     CDS             3864..4808
                     /locus_tag="PLL28_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70361.1"
                     /translation="MRAFTFFILQLSFFLIYAQPCDDRNTEYIPIADLGSSTFQGYQG
                     GLYPNGSNAIPMQHFQEGLSVASHIKPLNAEGKEDSISGKIGFLILGYSTAAMTGRFF
                     RDVVAIKSPDTKLQVVIGAQGGRDINSMTDSLSSYWDGVDSALHSNTITAAQIQLIWI
                     STGDILTYSLPFPQQSLQQVDKYALVLKQIKLQYPNARIVFLSDRPFAGYIGVNGMGP
                     KELAEPTAYFHSWTVKWVIEHQMNNDAGFTKEEIPFIDWGPTLWTNGSKGDKQGYTWN
                     CDDAGKGGIHASSKGRMKEATRMYVYFSNHPYTEQLFK"
BASE COUNT         1508 a          942 c          984 g         1443 t
ORIGIN      
        1 ttttcacata aagagatttc ggagcaacta aacatcagtg aaggaacgtc taagagtcag
       61 ttggcacgtg cacgctatat actcatggaa atggtagaac agcgtacagg tctcaaacgc
      121 aaagcaattg gagaataaca accaccatat cgatagaatt tttaaaaacg gcttaaagca
      181 ctattcagct cagccatctg cggatctttg gaaccgcatt gcagatgaat tggataatgg
      241 gatgcccgct aaatcctctc gtgctcaatg gtatcgatat gcagcaatgt tcttagtatt
      301 gttgtccgta gcattgatca gcgtaccggg caacagacat acccctcctg ccatatttgc
      361 tttcgacaat aataccggca atagctatgc ggcccctatt tcacatactg caatgccttc
      421 gtttaccgca ttgatccctg aaataactca agtggttgct gataatattt cgctgagaag
      481 aactccaagc gaatccgttt cctttgagaa tcccgttcag caaattacta ctgccgactt
      541 gtcgataaaa actcatccaa aagatgcatt tatttatctc cctgcagagc cggaaaatga
      601 aaccttagct gccgcaagtc aagatgtatt cgatcgcttc tacaataatg atgaacccat
      661 cgatgtgatg gcatacaatt attttggtga tgatgatcct gaagctgatt tttttgaagc
      721 gccttcttat aaagcaaaat accgcgatta cgatatgcgc ggattatata tcggcgctac
      781 aggtagctat aatcaaactt cattattgga atccggcaat gtatttaaag gcgagagacc
      841 aattcaacct tcgctgaaat ttggaactac caaaggtctt agggttggtt ataatatcaa
      901 caatagtttc ggtatcgaag ctgaatatgt ttataatgca ctgcaaggac agaattatgt
      961 gatgtctgaa ggtgacgata tcgttgaaaa atcactcagt ttaaactatg accttattcc
     1021 agtagtagct aaggtgaaaa ttggacatat ctcagatatt accaataaac ctgtagtgtt
     1081 gaattatact gccggtgtgc aatacggtat gttacgcgaa gcacgtatgc cacaagacaa
     1141 acgctatgaa gaatctgcca tggaattgtt taaagaaact gatgtatctg tggttttagg
     1201 attggaatat gatgtgtttg ttcaggataa tatcgtgata tcagcaggcg cccgcggtac
     1261 cttctctaat gatatcagca cgcatattga acctttaaat gactacgcca agcgcaattt
     1321 tgtcttcgga ttgcacggtg gcgttagtta tgttttcagg taatacctga actccctgct
     1381 tatatttgtg ttcccttttg tatgcgtaag tgtttgattt atgtatgtct gctcattatt
     1441 cttattaccc gcttatcagc aggtaccatc tccgggcata taaccgatac caccggcgct
     1501 ccactaccct tttgtaatgt ttatctcagt ggaactactt atgctgttcc tgcaaatgcg
     1561 gaaggatttt atcagcttac tgctccaaat ggagaatacg tgatagtatt tcaatttata
     1621 ggatacagcc gcaaggaaat acctatcacc atttcagata agccactaat gtttgatgtt
     1681 gttttacagg aagaaatgac cgaattgcaa acgatagtaa ttggtcctga tcatgaagat
     1741 cctgcatacg ctgtaataaa acaggccata gaacgacgtg cagatcactt gaaagaagtg
     1801 aatgcataca gttgtgatgt atatatgaaa ggacttcaaa aattgaatga agcgcctgat
     1861 caaatcatgg gcatcaagtt aaatacggtt tttgatgtcg attcgaataa tacaggtatt
     1921 atttacttgt cggaatcggt ttcaagcttc aattataaat atcccgatca aaccaaggag
     1981 atcatgaaag cttcggttgt aagtggcgac aaaaatcaat tcagctggaa tgatgctgcc
     2041 ggcatgcaaa tgaattttta tcaaaatctg gaaacacttc aagggttttc gcaacgtggg
     2101 tttgtttctc ctattgccga caatgcattg tttttctaca cctatcactt actcggcaac
     2161 acgtttgaaa acaatcgcga aattttcaaa atacaagtta cgcctaagcg ctccggtgat
     2221 cctgcctaca cgggggtaat ttatatcacc aacgacgatt atcgcatcac aggattggac
     2281 ctcacactaa ccaaacaaaa tggtattgaa ttcctggata catttaatgt gacacaggaa
     2341 tactattatg cagatcgcga acatttgatg ttgctttcga ataagttcag tttcaattac
     2401 ggcatgtttg gcattcaggg tgaaggctat tttcatgcgt attacaagaa ttacaccatc
     2461 aacccaatct ttgataagca tttttttgac ggtgaagtaa tcaggatcaa tgccggagca
     2521 aataagcaag acagcactta ttgggctggg atccgaccta tgcaactgac gaaggaagag
     2581 caattggatt atcatgaaaa ggattcattg gcaattttga aagaatcgcc ggcttatcag
     2641 gattcaatgg atcatatctt taataaattt tcacctgctg tattaataac cggttataat
     2701 tggcgcgaca gcaaatcaaa gtcaatgatt agcacaaata atattttcga cctgttgcaa
     2761 tttaatactg ttgaaaatta tgtgatacaa ccacgtctta cctacaccca ctggtatgaa
     2821 gataacagac gcttcgaaat tggcaccacg ctgcgttgga atacagcaac aaacggagcg
     2881 ctgatttcga tgcagtcacg ttacttactt gatcctgtgt cgcaggaaaa attatcacta
     2941 ttggcaggta gcagaatgca acaatttaat cccatgggta tatcgccatt ggtaaattcg
     3001 ctgtacacct tattgctgga acagaacagc atgaagttat atgcagaaga ttttattcag
     3061 tttgatgccg atcgggaaat gtataatggc ttctactttg gcataggagc atattatggc
     3121 aaacgttacc gccaatatga tgccattaat acagaacctt ggatagatga tcagtcgaaa
     3181 tatttttcca ataataatga cttcgtcata cacaatgcta ccgtatttga atgggggcca
     3241 atgtctgata ttttaatgtt gcatctccac attaaatatg tgataaagca aacctacatt
     3301 ctgcatcctg atgaaaaatt tattaatgag ccaaagtatc ctgttattga actggatctg
     3361 cgttcagcca atgtcacctt aaatgatgaa ttagatcaat ttaataaaat acaccttagt
     3421 gtttttgatc ggttacctct gtcacttgcc ggtaacctgg aatatatcgg aacagcaacc
     3481 gttcagtttg ccgattacga gcaattaatc gttgataaga tacatgctat cggcaatgaa
     3541 accaatgtaa gccgcttaca gaatgaaacc ttttatttgt tgccctatta ttatgccact
     3601 tcttcagatt atatgtttac aggccatgtc agctggcata ccgaaggatt tttgtttagg
     3661 aaattgccac tctttaagca attaaaaatg gaacctgtat tcagtgcaca tgtgttgaca
     3721 tcagacacca tgccgttcta tacagaattt acttttggcg tagaacatct ctttaaaatt
     3781 attcgcgtag accttgccat gacacctatg catgatgatt ttattcctcc tgcacggcta
     3841 tttatcggat ttggattttg atcatgcgag cattcacatt cttcatatta caacttagct
     3901 ttttccttat ctatgcgcaa ccttgcgatg atagaaatac tgaatacatt ccaatagcag
     3961 atttggggtc gtcaacattt caaggatatc aaggcggatt atatccaaat ggaagtaatg
     4021 caattccgat gcaacatttt caggaaggac ttagtgttgc atcgcacatt aaaccgctca
     4081 atgctgaagg aaaggaagat tcgatttccg gtaaaatcgg ttttctaatt ttgggatata
     4141 gcactgctgc tatgacaggg agattctttc gcgatgtggt agcaataaaa tcgccggata
     4201 caaaattgca ggttgtaatc ggtgcacagg gaggcagaga tattaattca atgaccgaca
     4261 gtttgtcttc ttattgggat ggtgtagatt ctgcccttca ctcaaataca attacggctg
     4321 ctcagattca attgatttgg atcagcacag gtgatatttt aacatacagc ctacccttcc
     4381 ctcaacagag tttgcaacag gtagataaat atgctttggt tttgaagcaa attaaattgc
     4441 aatacccaaa cgcaagaata gtttttttaa gcgacaggcc tttcgccgga tacattggcg
     4501 taaatggaat ggggccaaaa gaattagcag aaccaacagc atattttcat agttggacag
     4561 taaaatgggt gattgagcat caaatgaata atgacgcagg atttactaaa gaagaaattc
     4621 cttttattga ctgggggcca acgttgtgga ctaatggcag caaaggagat aaacaaggct
     4681 atacctggaa ctgcgatgat gccggaaaag gaggcataca tgcaagtagt aaaggaagaa
     4741 tgaaagaggc tacgcgtatg tacgtgtatt tttcaaatca tccatatact gaacagctgt
     4801 tcaaatgatc atcattcagt ttgcaaagta ggtggtgcat caatgacggc tttgatatca
     4861 ttgtatttat aaataca
//
LOCUS       DAOQHY010000219         4873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_10018_length_4873_cov_5.108344, whole genome shotgun sequence.
ACCESSION   DAOQHY010000219 DAOQHY010000000
VERSION     DAOQHY010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10018_length_4873_cov_5.108344"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="PLL28_13360"
     CDS             complement(<1..567)
                     /locus_tag="PLL28_13360"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNF70362.1"
                     /translation="MLILSNYIGGTLQSPVNGRYIDNYEPSTGSVYGKIPDSDEHDVN
                     LAVAAAKAAFPAWAAMDPASRSRQIHRIAEGINNRFEELAAAESRDSGKPVGLARRMD
                     ITRARDNFEFFAAAALQFASESHNQPGFINYTLRQPIGVVGCISPWNLPLYLFTWKIA
                     PALAAGNCVVAKPSEVTPVTAHMLAEICM"
     gene            881..1522
                     /locus_tag="PLL28_13365"
     CDS             881..1522
                     /locus_tag="PLL28_13365"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNF70363.1"
                     /translation="MAIRTTFYIDGFNFYNGIKSASKSINKDWKKYYWIDFVKFCQLF
                     LTDSHELVKVKYFSAPPFDSGKQSRQSALFKANKIINGDKLSIINGKYYKKPITCMAS
                     CKETFHISEEKRTDVNISVEMIGDCAFNNTDLIVLISADSDLVPPIEYVIKKFPDKKV
                     KVYFPPKRSSYDLKSMVNNNVVYLDKNEGKFKAAIMPDLVSNGVKHVNKPELW"
     gene            1818..3332
                     /gene="lysS"
                     /locus_tag="PLL28_13370"
     CDS             1818..3332
                     /gene="lysS"
                     /locus_tag="PLL28_13370"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNF70364.1"
                     /translation="MSQHLSEQEQIRRESLIAIRNMGIDPYPAAAFPITHNSSEILKG
                     FPTDPESYKEVSFAGRIMSRRIMGKASFAVLQDSAGKIQVYMKGEEICPPGDMSLYDE
                     LFKKHLDIGDIIGVTGYVFTTKTGETSIHVKTLTLLSKSLRPLPIVKTDADGNVFDAF
                     TDPEQRYRQRYLDLIVNPHVRDVFVKRSKLVNTMRSFMNQFGFLEVETPILQPLYGGA
                     AARPFKTHHNALDMTLYMRIANELYLKRLIVGGYDGVYEFSKDFRNEGMDRFHNPEFT
                     QVELYVAYKDYQWMMDFVEEMIEKVAMEVTGSTEVQVGEHVISFKRPWKRFTMFEAIE
                     HFTGIDISAMDEQQLFKTARELKVHVDGTMGKGKIIDEIFGEKCEPYLIQPTFITDYP
                     VEMSPLAKRHREKDGLVERFEAICNGKEIMNSFSELNDPIDQRHRFESQLELGKRGDE
                     EAMQLDEDFLRALEFGMPPTAGIGIGIDRLSMIMTNQHAIQDVLLFPHMRPENS"
     gene            complement(4152..4568)
                     /locus_tag="PLL28_13375"
     CDS             complement(4152..4568)
                     /locus_tag="PLL28_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70365.1"
                     /translation="MINGIHILIYSRKAAADKKFFKEVLKCPSVDVGGGWLIFGLPPS
                     ELAFHPSPEDISTELYLMCDNIHLFIEEMKKHNVECGRIGSQAWGFHTHIILPSGGKL
                     GVYQPTHERPKPMKVKSTSAKVAIEKKAAPKSGKNK"
BASE COUNT         1451 a          962 c         1063 g         1397 t
ORIGIN      
        1 catacatatc tctgccagca tatgggccgt tacaggtgtc acctctgaag gcttagccac
       61 cacacaattg cctgcagcca gcgcaggagc tattttccac gtaaacagat acaggggcag
      121 gttccagggc gaaatgcacc ctaccacgcc aattggctga cgcagcgtat agttaatgaa
      181 accgggttga ttatgacttt cagaagcaaa ttgcaacgcc gccgcagcaa aaaactcaaa
      241 attgtcgcgc gcacgggtga tatccatcct tctggccaga ccaacaggtt tacctgaatc
      301 acgcgattct gccgccgcaa gctcctcgaa gcggttgttg atgccttcgg cgatgcggtg
      361 aatctgtcga ctgcgcgagg ccggatccat ggcagcccat gccggaaaag ccgcttttgc
      421 tgcagctacc gccaggttta catcatgttc gtcggaatcg ggtattttgc catatacgga
      481 gcctgttgat ggttcgtagt tatcgatata acgaccattt acaggggatt gcagcgtgcc
      541 tccgatataa tttgataata tgagcaaaac gtatccgtaa aggtttacaa ttgtttcgaa
      601 cggcgaagat actaaaattc aggcaaaatt ccattgcagc gcgtatcctg aggtttcaat
      661 atataggtgc gttctgacaa atcccgagga caggcatttc cattcagatt taaatacggc
      721 atgaatgttg tatattttag taaacaactc attaaaacaa catatttgtc ctactaaaaa
      781 ttatttgctc ggaaataaat ttttgcaata actttgtacc aacgtcgctt atctatactg
      841 gataagttat accgccgaca tactggcggt ttttttttat atggcaatta gaactacttt
      901 ttatattgac ggtttcaatt tttataacgg aattaaatct gcgtcaaaat ctattaataa
      961 ggattggaaa aaatattatt ggattgattt tgtaaaattt tgccaactat ttttaactga
     1021 ttctcatgaa cttgtaaagg taaaatactt tagcgccccg ccttttgatt caggcaagca
     1081 aagtaggcaa tcggctttat ttaaagcaaa taagataatc aacggggaca agcttagtat
     1141 tatcaatgga aaatactaca aaaagccgat aacctgcatg gcctcctgca aagagacatt
     1201 tcatatctca gaagaaaaaa gaactgatgt gaatatttca gttgaaatga ttggtgattg
     1261 cgcatttaat aatactgatt taattgtact aatatctgca gacagcgatc tggttccgcc
     1321 aattgaatat gtaataaaaa aatttcccga caaaaaggtg aaagtatatt tcccacctaa
     1381 aagaagctct tatgatttaa aatcaatggt gaataacaac gttgtttatt tagacaaaaa
     1441 cgaagggaaa ttcaaagcag caattatgcc tgatttagtt tcaaacggag taaagcatgt
     1501 caacaagcct gaactctggt aattttttgc gttactctat gcggcagaag ctattccaat
     1561 tccacaaata tatcaggact cagcttaaaa aaatgtactc gtaaagggct ttggatattt
     1621 gacaaaaaag tggacttaaa gcaaatcata ccggaccttc aaaatactcc caaaccaggt
     1681 gtctattgca ttagtgcata caaagcaaag acacaagcaa attagtaaag gcatttcaaa
     1741 gcggagtcct gtatacagta ggaataaact aaataatttt tcccataacc gcttttcctt
     1801 ttctaccttt gcaccatatg tcgcaacacc tttcagaaca agaacagata cgcagagaaa
     1861 gtttgatcgc tatcaggaat atgggcattg atccatatcc cgcagctgca tttccaataa
     1921 cacataattc gtcagagatt ttaaagggat tcccaaccga tccggaatcg tataaggaag
     1981 taagctttgc aggccgcatt atgagccgtc gcattatggg gaaggcatcc tttgccgtat
     2041 tgcaagactc ggcaggaaaa attcaagtgt atatgaaggg tgaagaaatt tgccctcccg
     2101 gcgatatgag cttatacgac gagctattta aaaaacacct cgacattggc gatatcattg
     2161 gcgtaaccgg atatgttttc accaccaaaa caggcgaaac ttccattcac gttaaaacgc
     2221 tgaccttgtt gagcaaaagc ttgcgtccac tccccatcgt caaaaccgat gccgatggaa
     2281 atgtattcga tgcattcact gatccggaac aacgttatcg ccagcgctat ctcgatctta
     2341 ttgtaaatcc acatgtccgc gatgtatttg taaaacgcag caaactggtg aataccatgc
     2401 gcagttttat gaatcaattc ggttttcttg aagtggagac tccaatttta caaccactct
     2461 acggtggcgc tgctgcacgt ccattcaaaa cgcatcacaa tgcattggac atgacattgt
     2521 atatgcgcat tgccaatgaa ttatatttaa aacgtttgat cgttggcggt tatgatggcg
     2581 tgtatgaatt ctccaaagat ttccgcaatg aaggcatgga ccgttttcac aatccggaat
     2641 ttacccaagt tgaattatat gttgcctaca aagactatca atggatgatg gattttgtgg
     2701 aagagatgat agaaaaagtg gcaatggaag ttaccggttc aacagaagtt caggttggcg
     2761 aacacgtcat atccttcaaa cgcccatgga agcgtttcac catgtttgaa gctattgaac
     2821 actttacggg aattgatatt tccgcaatgg atgaacagca attatttaaa actgcgcgtg
     2881 aattaaaagt gcatgtagat ggcaccatgg gaaaaggaaa aatcattgat gaaatattcg
     2941 gtgaaaaatg tgaaccttat ctcatacaac caacattcat caccgattat cctgtagaaa
     3001 tgagtccgct cgctaaacgt caccgcgaaa aagacggact tgttgaacgt tttgaagcaa
     3061 tttgcaatgg caaggaaatc atgaattcat tctctgaatt aaatgatcct attgatcagc
     3121 gtcatcgctt cgagtcgcaa ctggaactcg gcaaacgcgg agacgaggaa gcgatgcaat
     3181 tggatgaaga ttttctgcgc gccctcgaat tcggtatgcc accaacggca ggtattggaa
     3241 ttggtattga ccgtttgtcg atgatcatga cgaatcagca tgcgatacaa gatgtgttgc
     3301 tgtttccgca tatgagaccg gaaaatagtt agcaagtttg taagtaagca ggtgtgcaag
     3361 tgtgcaggtg tgcatgtgtg aaagtgtgca tgtgtgcagg tgtgcaagta tgcaagtgtg
     3421 gaagtatgca agtgtgcagg tgtgtaagtg tgcaagtatg caagtgtgca agtgtgcaag
     3481 tgtgcaagtg tgcaggtgtg caagtgtgca ggtgtgcagg tgtgcaggtg tgcaagtgtg
     3541 caggtgtgca ggtgtgcaag tgtgcaggtg tgcaggtgtg caggtgtgca agtgtgcagg
     3601 tgtgcaggtg tgaaagtgtg catgtgtgaa agtgtgcatg tgtgaaagtg tgcaagtgtg
     3661 catgtgtgaa agtgtgcaag agggggaatg cttttgtggt tcgtttattt tcacgcggag
     3721 ggcgctgagg ggcggaggca cacggttggt ttgattttgc gaggtagtct taggaggtgg
     3781 ataaaccaaa gcacatgaaa acattcgaac ccgaacacat gcatacaaaa tagttttgtt
     3841 cgatttagga atggggtgat aatgattatt tgaattgtct caaatgatta atattcaatt
     3901 tgcatgcatc cgctgaacgc cgacaacttt tccggcacaa ctactccgca aactccgcca
     3961 actccgcgat taaacatttg caaattttga atttgaattt accatgaaaa tcagccggcc
     4021 ctgcaaagaa tcgtgcagaa ttgggagcat gtatttgtta gccgattagc gaggttggtt
     4081 atattatgta atgctcattt tacgttgaca gcccacttga tttctttgcc ttatttgagc
     4141 gttcgtgttt tttatttatt ctttccggat tttggagcag cttttttctc tatggcaact
     4201 ttcgctgatg tgcttttaac tttcattggc tttggtcttt cgtgtgttgg ttgatacaca
     4261 cccagctttc cgccacttgg taaaattatg tgggtatgaa aaccccaagc ctgacttcca
     4321 attcgcccac attctacatt atgcttcttc atctcttcaa taaacagatg aatattatcg
     4381 cacatcaaat acaactcagt ggagatatct tcaggagagg gatgaaatgc aagctcagac
     4441 ggaggcaacc caaatatgag ccagccaccg ccaacatcaa cgcttgggca cttcaaaact
     4501 tctttaaaaa attttttatc cgctgcagcc ttcctactgt aaataagaat gtgtattccg
     4561 tttatcattt tataaattgt ttaaatgttt gtagttttta cttaactaaa tcagattatc
     4621 aggttatccg ttaaccaata caaatgcaac tttcatgtag tcaagttaaa gttcatactg
     4681 tggtcaagta tggtgcataa cgttccgggg cttaaagaag tggcgattta gaatcacgaa
     4741 cggtcagcct gccacatatg gttatagaaa ccactatcat ttgattaacc actgaacccg
     4801 ccatttcttt taagcctttg tgcctgttgc acaactctcc aaatgtacac atttccatgt
     4861 tgattttcta ttt
//
LOCUS       DAOQHY010000220         4860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_10058_length_4860_cov_5.144641, whole genome shotgun sequence.
ACCESSION   DAOQHY010000220 DAOQHY010000000
VERSION     DAOQHY010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10058_length_4860_cov_5.144641"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..549
                     /locus_tag="PLL28_13380"
     CDS             304..549
                     /locus_tag="PLL28_13380"
                     /inference="COORDINATES: protein motif:HMM:NF016141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HNF70366.1"
                     /translation="MDNFLYFILLGAIAGYVSGKLIKGESFGLLGNIVVGVVGAVLGG
                     WIFDQVGIQEANILYSLAAAVVGSVAFLLIIGLLKRK"
     gene            complement(550..969)
                     /locus_tag="PLL28_13385"
     CDS             complement(550..969)
                     /locus_tag="PLL28_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNF70367.1"
                     /translation="MKRVTGIGGIFFRAKGEVKELNSWYEKHLGFDYINSNGYPFWAW
                     RSMEDPEKIGASIWSIFPRDTKYFGSNDQQFMFNFRVENMVELIETLRSEGVEIAGEI
                     QEFEYGKFCWIIDPDGNKIELWEAPENPGFEGGMPAK"
     gene            complement(1003..3303)
                     /locus_tag="PLL28_13390"
     CDS             complement(1003..3303)
                     /locus_tag="PLL28_13390"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF70368.1"
                     /translation="MRSLFIKFQLFCLLQFILIAGNAQSITGNITDATTREILPGVNI
                     TIADHGGVSSDENGNYNLQLPPGKYVLNFSFLGYETKQITVVLGKDDIRQLPVILEPA
                     SQLLDPIVVSGSLFEKKQSEETISMVIADQRLLEHTNTTNLSDAITKVPGVYMMDEQA
                     NIRGGTGFTYGAGSRVMLVVDDQILLAADRGDAKWNFVPMENVEQIEIIKGASSVLYG
                     SSALNGVIAVRTAWPGLTPETNISTYHTTFDKPALADAAWWDHAPTQSGISVSHRQKF
                     ENMDLVIGGNAASHDSYLLGQYSSRARLNWKTRFHPDAHDQLTWGVNGNLMSDREGLF
                     FLWQDADTGGFQPFQGYLDTTTTTLLNWQYKWATIDPWLNYYDKHHNSHKFKMRFYGS
                     DVIYTDTTDGNAWLLNADYQFFREFASDVVLTAGATGYYFDVDDHDVGIHDGISAGAY
                     VQLEKKLFDRLIINAGMREELYQVDTAEGASVPIFKGGLNYRLGASTNIRASFGQGYR
                     FPSLVEKYARSNLGTLFIFPNPDLKPEYGWNTEIGFIQAINRDAWRSTVDVAFYLTEY
                     FDMTEFTFNFYSGEGAGFKSLNTSRARIGGMEIIFKGDMELFGNRLTWIGGYNYIYPA
                     DISGDSSNLEFGHFMQNFIDGFTASSGDSVFLESVLKYRFRHTGRLDLDYETDKWLFG
                     ADVNYYSLMENLDAIYISFIPGIVEYREDHMHGDWIADARVGYKINKKMQVQLLGKNI
                     FNNMYALRPAKYDPPRSFTVQWRVEL"
     gene            complement(3312..4706)
                     /locus_tag="PLL28_13395"
     CDS             complement(3312..4706)
                     /locus_tag="PLL28_13395"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNF70369.1"
                     /translation="MRLRLLAALACITFTAFQQTANAQTAPAKTLDDKAAFNAIFDEV
                     MNNGEAYDNLEVLCKDIGHRLAGSPQADMAVRWSYDMIKKYGFDSVWLQPVTVPHWVR
                     GEQEYGEIMNVGKVNMLALGGSVATPIEGITDNVIEVRDFKHLEELGTAGIKGKIVFF
                     NYPFPQNVVNSFEGYGDAGPYRWHGANDASKYGAIAVVIRSVGSSHDDFAHTGSTHFD
                     DGVKKIPCAALSNIDADMLHNALQKDPSTKFKMIMSCAMLDSVQSYNVIAEIKGSTFP
                     KEVIVVGGHLDSWDVGEGAHDDGAGCMQSVEVLRAIQAAGLKPKRTIRCILFMNEENG
                     NMGGKTYGKYASATKDEKHIAALESDAGGFSPRGFSLDSAMLARPEMEQLKELMLKYG
                     VYSFEEGHGGTDIGPMEKAGTHLIGLVPDSQRYMDLHHTNNDVFEQVNKRELHMGASV
                     MAMMCWWISEHGVK"
BASE COUNT         1375 a         1163 c          854 g         1468 t
ORIGIN      
        1 tcgcctcacc ccccgccctc tccatctcct accgtcgata gagagggagc tgattgcgct
       61 tcggaattaa aatattgcaa agcaatataa ttgcattccc tattaacgcg ccagcgttaa
      121 tggcaccacc acttgctaat ttgctaattc tcaaacctgc taactaagaa gataaacccg
      181 atgcaagcaa tcacccccat ttgctaatca gctaattctc aaatttgcta attaactccc
      241 caaattcctc tctcctttct cctttctcct ctttcctctc tcctattaat tatctttgcc
      301 tccatggaca actttcttta tttcattctt cttggcgcga ttgccgggta tgtgagtggt
      361 aagctaatca aaggtgaaag ctttggattg ctgggaaata ttgtagttgg tgttgttggc
      421 gctgtgttag gaggatggat cttcgatcag gtaggtatac aggaagccaa tatcctttac
      481 agtctggcag cagctgtagt aggatcagta gcatttctgc tgatcatcgg attactgaag
      541 cgcaaataat tatttagcag gcattccacc ttcgaagcca ggattttcgg gagcttccca
      601 caattctatt ttattgccat cagggtctat gatccagcag aatttaccat attcgaattc
      661 ctgaatttca cctgctattt ctacgccttc gctcctcaat gtttcgatga gttcaaccat
      721 attttctaca cgaaaattga acatgaactg ctgatcattg ctaccaaagt actttgtatc
      781 acgaggaaag atgctccaga tggaagcgcc aattttttca ggatcttcca tgctgcgcca
      841 cgcccagaat ggatatccat tagaattgat ataatcgaaa cccaaatgtt tttcatacca
      901 gctatttaat tctttcactt cgccctttgc gcgaaaaaaa ataccgccta tgcctgttac
      961 tcttttcatg atatcagatt tatgatgttg aatatactga aattacaatt ccacccgcca
     1021 ttgcacagtg aaacttcttg gcggatcata ttttgcaggt cgcaaggcat acatattatt
     1081 aaaaatattt ttgcccaata attgcacctg cattttcttg tttattttat accctactcg
     1141 tgcatctgct atccaatcgc catgcatatg atcttcgcga tattctacaa tgcccggaat
     1201 aaatgaaata tagatggcat ccagattctc catcaggctg taatagttta catccgcacc
     1261 aaacaaccat ttgtctgttt cgtaatccaa atccaatcga ccggtatgac ggaagcgata
     1321 ttttaataca gattccagaa aaacggaatc accggaagaa gctgtaaatc cgtcaataaa
     1381 attctgcata aaatgtccga attcgaggtt ggaagaatct ccggaaatat ctgcaggata
     1441 gatataatta tatccgccta tccacgtcaa cctatttcca aataattcca tgtcgccttt
     1501 aaaaattatt tccatgccac cgattcgtgc acgtgaagta ttcaaggatt tgaatcccgc
     1561 gccttcaccc gaataaaaat taaaggtgaa ttcagtcatg tcgaaatatt ctgtgagata
     1621 aaaggctaca tcaacagtac ttcgccaagc atctctgttg attgcctgaa taaaaccgat
     1681 ttctgtattc caaccatatt ccggttttaa atccgggtta ggaaaaataa acaaggtgcc
     1741 gaggttagac cgcgcatatt tttcaaccaa actcggaaag cgatatcctt gtccgaagga
     1801 agccctgata tttgtagatg cacccaatcg ataatttaat ccacccttga aaataggtac
     1861 agatgcccct tcagcagtat ccacctgata cagttcttca cgcattccgg cgttgatgat
     1921 taatctgtcg aaaagtttct tttctaattg cacataagca cctgcactga tgccgtcatg
     1981 aatgccaaca tcatgatcat ccacatcaaa ataatatcct gttgctccgg cagttaatac
     2041 cacatctgaa gcaaattctc tgaaaaattg atagtcggca ttcagcaacc atgcattgcc
     2101 atcggtggtg tcggtgtaga taacatcaga accataaaag cgcattttaa acttatgact
     2161 gttatgatgt ttatcataat aattcagcca cggatcgata gtagcccatt tatattgcca
     2221 gttgagcaat gtggtagtag tggtatccaa atatccctga aagggttgaa atcctcccgt
     2281 atctgcatcc tgccacaaaa aaaagaggcc ttccctatcc gacatcagat ttccattcac
     2341 gccccaggtc aattggtcat gtgcatcagg atgaaatcgc gttttccaat ttaatcgcgc
     2401 acgagaggaa tattgtccta ataaatatga atcatgcgat gctgcatttc cacctatcac
     2461 caaatccata ttttcgaact tctgtcgatg gctaacactt ataccacttt gtgtaggcgc
     2521 atgatcccac catgcagcat ctgccagtgc cggtttatcg aaagtggtat gataagtgct
     2581 gatatttgtt tccggtgtca accccggcca agccgtgcgc acagcaatta cgccatttaa
     2641 tgcactgctg ccatataata ccgatgaagc ccctttgatg atttctatct gctccacatt
     2701 ctccattgga acaaaattcc attttgcatc accgcgatct gctgccagca atatctgatc
     2761 atcaacaacc aacataactc tgcttccggc gccataggtg aatcctgttc ccccgcgaat
     2821 attggcctgt tcatccatca tatacacacc cggcactttt gtgatcgcat cacttaaatt
     2881 agtagtgttg gtatgttcaa gcaatcgttg atcggcaata accatgctga tagtttcttc
     2941 gctttgtttc ttttcaaata aactaccact cacaactatc gggtccaata attgcgaagc
     3001 cggttctaaa attacgggta attgcctgat atcgtccttt cctaaaacta ctgttatttg
     3061 cttcgtttcg tatcccagaa aagaaaaatt cagcacatat tttccgggag gcaattgcaa
     3121 gttataattt ccattttcat cagaacttac accaccatga tccgcaatgg taatatttac
     3181 tccaggcaat atttctcttg tggtagcatc tgtaatattt cccgtaatag actgcgcgtt
     3241 tcctgcaatc agaatgaatt gcaacaaaca gaacagttgg aattttatga ataggctacg
     3301 catcttaaaa attattttac tccatgttcg cttatccacc agcacatcat ggccatcact
     3361 gaagcgccca tatgtaattc tcgtttattt acttgttcaa atacatcatt attggtatga
     3421 tgtaaatcca tgtatcgctg tgaatcaggt accaatccga ttaaatgtgt ccctgctttt
     3481 tccataggac caatatctgt tccaccatga ccttcctcaa aactataaac tccatattta
     3541 agcatgagtt cttttaattg ctccatctca gggcgtgcaa gcatcgctga gtccaaacta
     3601 aatccgcgtg gagaaaatcc gcctgcatca ctttccagtg cggcgatatg tttttcatcc
     3661 ttggtggcag aggcatattt tccataagtt ttacctccca tattaccatt ctcttcattc
     3721 ataaaaagta tgcatcgaat agtgcgttta ggttttagtc ctgccgcttg tattgcgcga
     3781 agtacttcaa cactctgcat gcaaccggca ccatcgtcgt gcgcaccttc accaacatcc
     3841 cagctgtcta aatgcccgcc tacaacaatc acttcctttg gaaaagtaga cccttttatt
     3901 tctgcaataa cattgtatga ttgcaccgaa tcaagcatgg cgcaactcat gatcatttta
     3961 aattttgtgg aaggatcttt ttgcaaggca ttatgcagca tatctgcatc gatattgctg
     4021 agtgctgcac aaggaatttt ttttacgccg tcatcaaaat gagtacttcc tgtatgggca
     4081 aaatcgtcat gagaagaacc aacgcttctg atcacaacgg caatagcacc atatttacta
     4141 gcatcattcg caccatgcca gcgataaggt cccgcgtcgc cataaccttc aaatgagttc
     4201 accacatttt gaggaaatgg gtaattaaaa aatacaatct ttcctttgat gccagccgtg
     4261 cccaattctt caagatgttt gaaatcgcgc acttcaatta cattatcggt aattccttcg
     4321 ataggcgttg caacactacc tcccaaagca agcatattca cttttccaac attcataatc
     4381 tcgccatatt cctgctcacc acgcacccaa tgtggaacag tcaccggctg caaccataca
     4441 gaatcaaatc cgtatttttt gatcatatca taactccaac gcactgccat atccgcttgc
     4501 ggcgatcctg cgagtcggtg accaatgtct ttacataata cttctaagtt atcatacgcc
     4561 tcaccattat tcattacttc atcgaaaatg gcattgaaag ctgctttatc gtcgagggtt
     4621 ttggcaggag ccgtttgggc gtttgcggtt tgttggaatg ccgtaaaagt gatacacgcc
     4681 aaagctgcca ataatcgtag tctcatccaa aagttaattt atctgctaaa ataatgaacc
     4741 tattcacaaa ggtggggttt ttaactgcat gacatgttag caacgattct gtttggcgat
     4801 ttggaacatt cagcgggatt gggcatactt gcaattacat tttacgcaga tgatgagata
//
LOCUS       DAOQHY010000221         4823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_10162_length_4823_cov_5.745596, whole genome shotgun sequence.
ACCESSION   DAOQHY010000221 DAOQHY010000000
VERSION     DAOQHY010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10162_length_4823_cov_5.745596"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1663)
                     /gene="ppk1"
                     /locus_tag="PLL28_13400"
     CDS             complement(<1..1663)
                     /gene="ppk1"
                     /locus_tag="PLL28_13400"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNF70370.1"
                     /translation="MAGKKQKKEKPKFINRDLSWLSFNERVLQEAEDKRVPLNDRIRF
                     LGIFSNNQDELFRVRVAVLRRMMRLQRGDLKQVDMNPSKVLNQIQKKVLVLKNRFDNA
                     YLQILEELKKHKIYILNEKEISTQQGEIIKNYFNQHVRQWLFPIMIDESKKMPLLNDK
                     SIYLAIRMVKKDKPLATRYSLIEVPDGDLPRFFVLPDDKEKKFVMLLDDIIRYNLDSI
                     YYIFNYDSIEAYTIKITRDAELDISTDLSQSLLEIIQKSVKRRNKGETTRFIYDAQMP
                     EEMLQFFARKMKLQRQDPIIPGARYHNFKDFMKFPDLGRDVLTYPELVPHHHPLLNTY
                     SSITSVLRKRDVLLHFPYQSFDHIIDLLREAAINPNVTTIKMTLYRVARHSSVVMSLI
                     NAARNGKQVTVVMELQARFDEENNIFWANRLQEEGVRVLFGSNNLKVHSKLCLINHHH
                     EGKDVQYAIVGTGNFNENTAKVYTDLFLLTAKPEVTKEVKKIFDIFENKFELGKYKHL
                     LVAPINLRSRLKLLINREIKFAKEKKKAAIFLKVNNLVDKEIIKKL"
     gene            1808..2977
                     /locus_tag="PLL28_13405"
     CDS             1808..2977
                     /locus_tag="PLL28_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNF70371.1"
                     /translation="MEQDRYALRGVSSQKEEVHKAIKNMDKGLFPNAFCKILPDLIAG
                     DPAFCNVMHADTAGTKTSLAYIYWKETGDLSVWEGIAQDALVMNLDDMACVGITDNII
                     ISSTIGRNKSIIPADVIAAVINGTNNFLANMQLHGVQIHHAGGETADVGDIVRTIDVG
                     ITAFGRIDRSKLIVNDIKPGQVIVGLASYGKSIYETAYNGGMGSNGLTSARHDVFHHQ
                     YADKYPESFSPEIPREVVYSGSKSLTDEITVNNEKITLGKLVLSPTRTYLPVIRTMLE
                     NHSDAIKGMIHCSGGGQTKVLHFADKVHIVKDNLFATPPLFDIIQQESGTSWKEMYRT
                     FNMGCRLEIYTDQNTAESLINIAKSFDIEAQIIGHVMPSENKKLTVKSAYGEFEY"
     gene            complement(2995..3858)
                     /locus_tag="PLL28_13410"
     CDS             complement(2995..3858)
                     /locus_tag="PLL28_13410"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNF70372.1"
                     /translation="MVLSTFLFAVMNVIVKYLHRLPSHELVLFRSLVMFVITAGMLYR
                     AKLQPFRYQPRWILIGRGVAGSLALLIYFFTIQHLPLASAVTLGYLSPIFTIIFASFL
                     LKEGMRWVQWVFFLVSFAGVVIMHGFESAEDTPLVLLGVLGAAFSGLAYNALRKSAGT
                     VPTLVVVFFLPLCTIPLVIPFCLHNWIMPVGYEWLLIIAMGAVTQAAQIYMTKAYQLA
                     KAGAIANYAYLGVVYALILGYFIFDERFAFIGVIGIGIVILGIILNYFYVNRVTSLKR
                     FVVYFRWFPGL"
     gene            4099..>4823
                     /locus_tag="PLL28_13415"
     CDS             4099..>4823
                     /locus_tag="PLL28_13415"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNF70373.1"
                     /translation="MRMLSLLLAIFFVGNAFGQSTATTTETEAPKLTEAEALEKFDRF
                     LSIVDDRYMDDVDAGKLVEDAIIEVLAQLDPHSVYFTEDELKEANEPLEGNFEGVGIQ
                     FNLLRDTIFVVSVISGGPSEKVGILAGDKIVQINEENVAGIGMSNKGVTDRLRGKKGT
                     DVRVYIERKGAKKPLEFKITRDVIPLYSIDAKYMASPETGYIRVSKFAATTTEEFQAA
                     VKELKSSGMKNLILDLRGNGGGYL"
BASE COUNT         1504 a         1125 c          879 g         1315 t
ORIGIN      
        1 ataatttctt tatgatctcc ttatcgacca ggttattcac tttcaaaaaa atagctgcct
       61 tttttttctc tttggcgaat ttaatttctc tgttaatgag caatttcaat cgcgacctta
      121 aattaattgg tgctaccaat aaatgcttat acttccctaa ttcaaactta ttttcaaaaa
      181 tgtcgaatat cttcttcacc tccttcgtca cctcgggctt tgctgtcagc agaaataaat
      241 ctgtatacac ctttgccgta ttctcattga aattacctgt tcctacaatg gcatattgca
      301 catcctttcc ttcgtgatga tgattgatca aacacaattt agaatgcact ttcagattat
      361 tgcttccaaa caacaccctc acaccttcct cctgcaatct gttggcccag aaaatattat
      421 tttcttcatc aaacctcgcc tgcaattcca taactaccgt tacctgcttg ccatttcgag
      481 cagcattgat caaactcatt accacacttg aatgccgcgc aactcgatac aatgtcattt
      541 tgattgtggt gacattcgga ttgatagccg cctcgcgcaa taaatctata atatgatcaa
      601 aactctgata cggaaaatgc aataacacat cacgctttct taataccgaa gtaatactcg
      661 aataggtgtt caacaatgga tgatgatgcg gaactaattc aggatatgtc aacacatctc
      721 ttcccagatc agggaatttc atgaagtctt tgaaattgtg atagcgcgca ccgggaatta
      781 tcggatcctg acgctgtaat ttcatcttcc gtgcaaaaaa ttgcaacatc tcttccggca
      841 tctgcgcatc ataaatgaat cttgttgttt cacctttatt cctgcgcttc acacttttct
      901 gtatgatttc gagcagactt tgcgacaaat ctgtactgat atccaactcc gcatcacgcg
      961 taattttgat cgtatatgcc tcgatgctgt cgtaattaaa aatataataa atactatcca
     1021 gattatacct gataatatca tccaacaaca tcacaaactt tttctctttg tcatcaggta
     1081 acacaaaaaa tcttgggagg tcaccatccg gcacttctat aagcgaatat cgtgttgcca
     1141 acggcttatc cttcttcacc atcctgatcg ccagataaat acttttatca tttaataaag
     1201 gcatcttctt cgattcatct atcataatcg gaaataacca ctgccttaca tgctgattaa
     1261 aataattttt tatgatttca ccctgctgcg tacttatctc tttctcattt aaaatataga
     1321 tcttgtgctt cttcaattcc tccaaaatct gcaaataagc attgtcaaac ctgttcttca
     1381 ataccaacac tttcttctga atctgattta acaccttcga tggattcata tccacctgct
     1441 tcagatcgcc gcgctgcaat cgcatcatcc tgcgcaatac cgcaacacgc actcgaaaca
     1501 actcatcctg attattcgaa aaaatgccca aaaacctgat cctgtcattc aaaggcaccc
     1561 gcttgtcctc tgcttcctgc aaaacacgct cattgaacga caaccaactg aggtcacgat
     1621 tgataaactt tggcttctct ttcttctgtt ttttccccgc cataacatta catgctgttc
     1681 atcacaaata tattcaggac taagatacgg gcaataaaca gccctttcct gattattcgt
     1741 ttaagttaat gttaatttat tgtttgggta acattagagg cacaaagcat ttatgtaatt
     1801 ttgcagcatg gaacaggatc gttatgcctt gcgtggcgtg tcgtcgcaga aagaagaagt
     1861 gcacaaagcc atcaaaaaca tggataaagg cctgtttccc aacgcttttt gcaagatctt
     1921 acccgatctg attgccggtg atcccgcctt ttgtaatgtc atgcatgccg atactgcagg
     1981 caccaaaaca tctttggctt atatctactg gaaagaaacc ggcgacctat cggtatggga
     2041 aggtatagcc caggatgcac tagtcatgaa tctggatgat atggcctgcg ttggcatcac
     2101 cgataatatt attatcagct caactatcgg aagaaataaa agcatcatcc cggccgatgt
     2161 aatcgctgct gtcatcaacg gaacaaataa tttcctcgca aatatgcaat tgcatggtgt
     2221 gcaaattcac catgccggtg gcgaaactgc cgatgttggc gatatcgtgc gcactatcga
     2281 tgtcggtatc acggctttcg gtcgcataga tcgcagtaaa ttgatcgtca acgatattaa
     2341 acccggacaa gtgattgttg gcctggcaag ctacgggaaa tcaatttatg aaactgctta
     2401 caacggaggt atgggcagca acggtcttac cagcgcccga cacgatgttt tccatcatca
     2461 atatgccgat aaatatcccg aaagtttcag tcctgaaatt ccaagagaag tagtgtattc
     2521 cggaagtaaa tccctcaccg atgaaatcac tgtcaataat gagaaaatta cactcggaaa
     2581 gttggtgcta tcccctactc gaacttacct ccctgtaatt cgcaccatgc tcgaaaatca
     2641 tagtgatgca attaagggta tgatccattg cagcggtggc ggacaaacta aagtgctgca
     2701 ctttgccgat aaagtgcaca ttgtaaaaga taacctcttc gcaactcctc cactttttga
     2761 cattattcag caggaatccg gcacttcatg gaaagaaatg taccgaactt ttaatatggg
     2821 ttgccgacta gaaatctata ccgatcagaa tacggcagaa tcactaatca atatcgctaa
     2881 aagttttgat attgaagctc aaatcatcgg tcatgtgatg ccatctgaaa acaaaaagct
     2941 tacggttaaa tcagcgtacg gcgaattcga gtattgataa aaccaatact tacatcaaag
     3001 gcccggaaac cagcggaaat aaaccacgaa tcgcttcaag gaagtaacgc gattgacgta
     3061 aaaataattg aggattatcc ccaatatcac aatcccgatt cctatcacgc caataaaggc
     3121 aaaacgctca tcaaatataa aatatcctaa tatcagggca tacaccaccc cgaggtaggc
     3181 ataattggct atcgctccgg ccttggccag ctgatatgct ttggtcatat aaatctgagc
     3241 tgcctgcgtt accgctccca ttgcaataat caacaaccat tcatatccta ccggcataat
     3301 ccaattatgc aaacaaaaag gtatcaccaa tggaatggta cacaagggca aaaagaacac
     3361 tactaccaat gtaggcaccg taccggccga ttttcttaat gcattataag ccaacccgct
     3421 aaaggcagca cccaacacac ctaaaagcac caagggtgta tcttctgccg attcaaatcc
     3481 atgcatgatt accacacctg caaacgatac caaaaagaac acccactgca cccaacgcat
     3541 accctctttg agtaaaaaag aagcaaaaat gatggtgaaa atgggcgata aatacccaag
     3601 cgttaccgcc gaagccaatg gcagatgctg tatcgtgaaa aaatagatca acaatgcaag
     3661 cgaacctgct acaccgcgac ctatcaatat ccatctcggc tgatacctga atggctgcaa
     3721 cttagccctg taaagcattc cggcagtaat aacgaacatg accagggaac ggaaaagcac
     3781 caattcgtgc gatggtaagc gatgcaaata cttcacgatc acattcatca ccgcaaataa
     3841 aaaggtggaa agcaccatga aaaataccgc cctgctatgt gtcgaggagc ccaaagttct
     3901 gttgagatac tatgcaaagt aaccaccatt gtttgtggaa tgttccaata gttatcacca
     3961 tatgcacaaa accctgcatt ttattaagac cggggttaaa acttgttaaa aggaacccat
     4021 tttggcacgg atatcgttta taatttaaaa tcaacctaat tttaaggcaa tcaaatactt
     4081 gaacaatcta tggtgaagat gagaatgtta tcccttctgc tggcaatttt ctttgtcggc
     4141 aacgcatttg ggcaatcaac tgcaacaaca accgaaaccg aggctccaaa gctcactgaa
     4201 gctgaggcgc ttgaaaaatt cgaccgattc ctttctatcg tagacgatag atatatggac
     4261 gatgtcgatg ccggcaaact ggttgaagat gcaataatcg aagtgcttgc acaacttgat
     4321 ccacactctg tttattttac ggaagatgaa ttaaaagaag caaatgaacc gcttgaaggt
     4381 aattttgaag gcgttggaat tcaattcaat ttgctgcgcg ataccatttt cgtggtgagt
     4441 gtaatttccg gcggaccaag tgagaaagtc ggcatacttg caggtgataa aatcgtacag
     4501 ataaatgaag aaaatgttgc cggaatcgga atgtcgaata aaggcgttac cgatcgcttg
     4561 cgtgggaaaa aaggcactga tgtgcgtgta tacatcgaac gcaaaggcgc caaaaaaccg
     4621 ctcgaattta aaatcacgcg cgatgtaatt ccattataca gcatcgatgc gaaatatatg
     4681 gcgagtcccg aaaccggata tatccgtgta agcaaattcg ctgcaaccac taccgaagaa
     4741 tttcaagccg cagtgaaaga attaaaatct tcaggcatga aaaatctcat cctcgatctg
     4801 cgtggtaatg gcggaggtta tct
//
LOCUS       DAOQHY010000222         4816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_10192_length_4816_cov_6.121823, whole genome shotgun sequence.
ACCESSION   DAOQHY010000222 DAOQHY010000000
VERSION     DAOQHY010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10192_length_4816_cov_6.121823"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1556)
                     /locus_tag="PLL28_13420"
     CDS             complement(<1..1556)
                     /locus_tag="PLL28_13420"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type H(+)-translocating pyrophosphatase"
                     /protein_id="HNF70374.1"
                     /translation="MDVNMILIAIPVLGLIALLFTYMKSNWVARQDPGDAKMQGIAGA
                     IADGAMAFLRAEYRILAIFVVIVAILLGLSGASEENSSALVGVSFVVGASCSALAGFI
                     GMRVATKANVRTANAARGGLGKALEIAFSGGSVMGMGVVGLGILGLGTLLLIYSNIYG
                     FDSQEAAIKVINVVTGFSFGASSIALFARVGGGIYTKAADVGADLVGKVEAGIPEDHP
                     LNPATIADNVGDNVGDVAGMGADLFESYVGSIVGTMVLGTAFMAGTIGVGTSFMEDGF
                     NGLSAVLLPLVLGGVGILTSIVGTFFVRVKEGGNPQKALNGGALGSSIAMVILSYFII
                     TWMLPEKFTFTDVLYGGKERTVTAIGIFIATVVGLIAGSAIGFITEYYTGTGKKPVMK
                     IVQQSSTGSATNIIAGLSVGMMSTAIPTLIISAAIIVSFMFGGLYGIAIAAVGMLANT
                     GIQLAVDAYGPISDNAGGIAQMADLPKEVRGRTDKLDAVGNTTAAIGKGFAIGSAALT
                     ALALFGAFMQV"
     gene            1676..2494
                     /locus_tag="PLL28_13425"
     CDS             1676..2494
                     /locus_tag="PLL28_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70375.1"
                     /translation="MNPGKLLTCAILCISLSVHAQTGYSDAGASANSMGNTGIANPDV
                     FSAQNNQAAMAFAENASLGISTQNYFLMDEGLNAFYAAGIIPVQRAGNFGITAHYTGD
                     ATFNQTKIGLGYGRKLADVLAIGVQLDYVGTNTSEVGNGTAFTFDIGVLYKPTKTLSI
                     GAKAFNPVRVKNGLEYAEELPAIITAGLQWQPSEFVRVCLEGEQNIDQDLRIKTGIEY
                     HIIDVLYLRGGYISNPSMFTTGIGLKLKTFMLDAGAQFHQQLGVTPGVSLQYAF"
     gene            2508..4595
                     /locus_tag="PLL28_13430"
     CDS             2508..4595
                     /locus_tag="PLL28_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70376.1"
                     /translation="MKIFHPHIFICTSFMMMFSFAHAQENKGEGKLLEDDIIEGAAEK
                     TDNADLSIDTYQENLNYLLQNPISINTASYIELRNSGLFTEVQIRDLRRHIESYGPLL
                     NLYELQTIPSFYPEDIARILPFITLNQVSRDKVAFIDQLYLGDYQYYTRLSRYLETQD
                     GYVADSAGNTSYAGNNLRVYNRFRYNYNNKLSYGFTAEKDAGEAVFGPTQPYGFDYYS
                     AHFFKKGTGTMRALALGDFELRIGQGLMMWSGFGFGKSVFPLAIRRAGPVLDSYTSTN
                     ENRFLRGAGATLAYGRWQFTPFISYKSIDANVALLDTVSDEVIQVSAIDESGLHRTES
                     ELERKDALKELMGGFDLTWYKDQVNIGFSAIGYHYSAPLQPTISPYEIYNFTGQQLIN
                     FGTHYNFLWRNMLLFGETAMSDNGKAGTLNGVIIPLDPKMDIAILHRYFSPDYQTLYA
                     ATFSDATLPQNENGTYFGTEIKPIKGWKLSGYVDLYKHQWLEYQTDAPGYGTDLLAQL
                     TWQPSRSFNTYVRYKRENSDRNSTDDIIDAENQLNVVSDLMRSSIRWHADYDVNKAIT
                     VKSRIEVSFYEETYGIPERGYLVFQDFNYKPLSSPIGFATRFAIFNTDSYNTRIYAYE
                     TEVLYAYSIVGLSGRGTRAYLMFTWSPFKWMDVWARVANTWYSGDEPLGSGDNTFPGN
                     TRSDAKLQVRIKW"
     gene            complement(4596..>4816)
                     /locus_tag="PLL28_13435"
     CDS             complement(4596..>4816)
                     /locus_tag="PLL28_13435"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNF70377.1"
                     /translation="GATIGKLLTGIRVRRKDGGKISIGTAFLRHIGRIISTMIFMLGY
                     LLAFIDGKRQTLHDKIANTMVLKKGTGI"
BASE COUNT         1470 a         1038 c          979 g         1329 t
ORIGIN      
        1 acttgcataa atgcaccaaa taatgccagc gcagtaagtg ctgcagagcc gatggcaaat
       61 ccttttccga tagctgctgt tgtatttcct acagcatcta atttatctgt tctgccgcgc
      121 acttctttag gaagatcagc catttgagca ataccacctg cattatcaga aatcggtccg
      181 taggcatcca ctgctaattg aataccggta ttagcaagca tacctacagc ggcaatggcg
      241 atgccatata atccgccgaa cataaaagaa acaatgatag ctgcagaaat aattaatgta
      301 ggaatggcag ttgacatcat acctacactt aatccggcaa tgatatttgt tgcagaacct
      361 gtagatgatt gctgaacaat tttcataaca ggtttttttc ctgtaccggt ataatattca
      421 gtaataaatc caattgcaga acctgcgatg agacctacaa ctgttgcaat aaaaatacct
      481 atagctgtta ctgttcgttc tttacctcca taaagcacat cagtaaacgt aaatttttca
      541 ggcaacatcc atgtgatgat aaagtatgat aaaatcacca ttgcgattga tgaacctagt
      601 gcaccaccat taagtgcttt ttgaggatta ccaccttctt tcacacgaac aaagaaagta
      661 cctacaatag aagtaaggat accaacacca cctaaaacaa gcggaagtaa tacagcagac
      721 aaaccgttaa atccatcttc cataaaagaa gttccaacac caatagtacc tgccataaat
      781 gctgttccca aaaccatagt tccgacaata gaacctacat aactttcaaa taggtcggca
      841 cccataccgg caacgtcgcc aacattatca ccaacgttgt ctgcaatggt agcaggattc
      901 agtgggtgat cttccggaat accggcttca acctttccaa caaggtcggc acccacatcg
      961 gcagctttgg tatagattcc accacctaca cgggcaaaaa gtgcaatcga tgatgctccg
     1021 aatgagaatc cggtaactac attgatcacc ttgattgctg cttcctgaga atcgaaccca
     1081 taaatatttg aatagatgag caataaggtt cccaagccaa gaattcccag acctacaaca
     1141 cccataccca taactgatcc accgctgaag gcaatttcaa gggcttttcc aagtccccct
     1201 cttgcagcat ttgcagtgcg cacatttgct tttgtagcca cgcgcatacc gataaaaccg
     1261 gccaatgctg agcatgaagc accaacaaca aaagatacac caaccagtgc tgaagaattt
     1321 tcttcacttg cacccgaaag ccctaatagg atagcaacaa tgacaacgaa aattgccaga
     1381 atgcgatatt cagctctcag gaaagccatg gcaccatcag cgattgcacc ggctatacct
     1441 tgcattttag catctccggg atcttgacgt gctacccagt tgctcttcat gtaggtaaat
     1501 aacaatgcga tgagcccgag caccggaatc gcaattaaaa tcatattaac atccatatct
     1561 cagtggtttt ataggtttat tgaagatgct aaaataatca tagtcagcca aatgcccatt
     1621 ttcggaatgt tgatagcctg aaatgtcttg caggatttct tatatcttta cctgtatgaa
     1681 cccgggaaaa ttactcacat gtgctattct gtgcattagc ttatctgtgc atgcccagac
     1741 cggctactct gatgcgggag cctcggccaa tagtatgggg aatacaggta ttgctaaccc
     1801 cgatgtattc agcgcccaaa acaatcaggc agcaatggcc tttgcagaga atgcttcatt
     1861 aggcatcagc acacagaatt atttcctcat ggatgaagga ttgaatgcat tttatgctgc
     1921 aggaattatt ccggttcaac gagcaggaaa ttttggcatt accgctcatt ataccggtga
     1981 tgcaacattt aatcaaacaa aaataggttt gggttatggt cgcaagctgg ctgatgtgtt
     2041 agctattgga gtgcaattag attatgttgg cactaacaca tctgaggtag gtaacggaac
     2101 tgcatttaca tttgatatcg gcgtgttgta caaaccaact aaaactttat ctatcggcgc
     2161 taaagccttt aatcctgtaa gagtgaaaaa cggacttgaa tatgctgagg aacttcctgc
     2221 aattattacg gcaggattgc aatggcaacc ctcagaattt gtacgggtgt gtttagaagg
     2281 agaacaaaat attgatcagg atctgcgtat aaaaacagga attgaatacc acattatcga
     2341 tgtattatat ctcagaggtg gttacatctc caacccatct atgttcacca cgggaattgg
     2401 gttgaaatta aaaactttca tgttagatgc cggtgctcag ttccatcagc aactcggcgt
     2461 tacaccgggt gtgtctttac agtatgcatt ttgatgaata ataatccatg aaaatatttc
     2521 atcctcatat cttcatttgc acttctttta tgatgatgtt ttcttttgcc catgcacagg
     2581 aaaacaaggg tgagggcaaa ttactcgaag acgacataat tgaaggcgcc gccgagaaaa
     2641 cagataatgc cgatttgtct atcgacacct atcaggaaaa tctcaactac ttacttcaaa
     2701 atccaatttc gattaataca gcatcttata tcgaattacg caattcggga ttatttactg
     2761 aagtgcagat acgagattta cgccgacata ttgaatctta cggcccgtta ttaaatttat
     2821 acgagctgca aacgatacct tctttttatc cggaagacat tgcacgaatt ctgccgttta
     2881 ttacattgaa tcaggttagc agagacaagg ttgcatttat tgatcaatta tatcttggcg
     2941 attatcaata ttataccagg ttaagccgct atctggaaac tcaggatgga tatgttgcag
     3001 acagtgcagg caatacctcc tatgcaggaa ataatttacg tgtatacaat cgtttcaggt
     3061 acaactacaa taataaatta tcatatggtt tcacggcaga gaaagatgcc ggagaagcag
     3121 tattcgggcc cacacaacct tatggtttcg attattattc tgcccacttt ttcaaaaagg
     3181 gcacaggcac catgcgcgca cttgctttgg gtgattttga attacgcatc ggtcagggat
     3241 taatgatgtg gagcggtttt ggttttggaa aaagcgtctt ccctttggca atcagacgcg
     3301 ccggaccggt gctcgacagt tacacctcaa caaatgaaaa ccgtttcttg cgcggagcgg
     3361 gagctacact tgcttacggt cgctggcaat tcacgccatt tatttcatat aaatcaattg
     3421 atgcaaatgt tgcattattg gatacagtaa gtgacgaagt aatacaagtg tcggcaattg
     3481 atgagagtgg attacatcgc acagaaagtg aactcgaacg caaggatgca ttgaaggaat
     3541 taatgggcgg atttgatctt acctggtata aggatcaggt gaacatagga ttttctgcta
     3601 tcggttatca ttattctgct ccgttgcaac caacaatttc accttatgaa atatataatt
     3661 tcaccggtca gcaattgatc aacttcggca cgcattataa ttttctctgg cgaaacatgc
     3721 tgctctttgg tgaaacagcc atgagtgata atggaaaagc aggaacgctc aatggggtaa
     3781 taattccgct tgatccgaaa atggatatcg ccatactgca tcgatatttt tctcccgatt
     3841 atcaaacttt atatgctgca actttttctg atgctacctt gcctcaaaat gaaaatggta
     3901 cttacttcgg cacagaaata aaaccaataa agggatggaa actttcaggg tatgtagatt
     3961 tgtataaaca ccaatggctc gaatatcaaa ccgacgctcc cggttatggc acagatttgc
     4021 ttgctcaatt aacatggcaa ccatcgcgaa gtttcaacac ttatgtgcgc tataagagag
     4081 aaaatagcga cagaaacagc accgatgata ttattgacgc agaaaatcaa ttaaatgttg
     4141 tgagcgattt aatgcgcagt tctataaggt ggcatgccga ttatgatgta aataaagcaa
     4201 ttactgtaaa atcgcgcata gaggtgagtt tctacgagga gacctatggc attcctgagc
     4261 ggggatattt ggtattccag gatttcaatt ataaaccatt gagcagccct ataggttttg
     4321 ctacgcgatt tgcgattttt aataccgatt cttacaacac ccgtatttat gcatatgaaa
     4381 cggaagtgtt atatgcatat tccatcgttg gattaagcgg tcgcggcacc agggcatatc
     4441 tgatgttcac ctggtcgcca ttcaagtgga tggatgtatg ggcaagagta gccaacacct
     4501 ggtattcagg ggacgaacct ttaggttccg gcgataatac gtttccggga aatacccgca
     4561 gtgatgcgaa actgcaggta aggattaaat ggtgatcaga tgcctgttcc tttcttcagc
     4621 accattgtat ttgcaatctt atcgtgcaaa gtctgacgtt ttccgtcgat aaatgcaagc
     4681 aagtatccca acatgaaaat catggtcgat atgattcttc cgatatggcg aagaaacgca
     4741 gttccaatgc tgattttgcc accatcttta cggcggacac ggatgcctgt caacaattta
     4801 ccgatggtag cccccc
//
LOCUS       DAOQHY010000223         4802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_10236_length_4802_cov_5.924584, whole genome shotgun sequence.
ACCESSION   DAOQHY010000223 DAOQHY010000000
VERSION     DAOQHY010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10236_length_4802_cov_5.924584"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            450..1463
                     /gene="nadA"
                     /locus_tag="PLL28_13440"
     CDS             450..1463
                     /gene="nadA"
                     /locus_tag="PLL28_13440"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNF70378.1"
                     /translation="MIIGGEKVVDIEKLERTGFVDVDVDPSLDLFAEINKLKKEKNAV
                     ILAHYYQEPDIQDIADYIGDSLGLAQHAEKTNADMIVFAGVHFMAETAKILNPDKKVI
                     MPDAKAGCSLADSCPPALFRKFKEQHPGHTVISYINCTAEIKALSDIICTSSNAVKIV
                     ESLPADEPIIFAPDKNLGRYIEKKTGRKMVLWEGSCMVHEIFSLEKITKLKHRHPNAK
                     VIAHPECEEPVLAIADFIGSTTGLLKYATTDSAQEYIVVTETGIIHQMQKSAPQKTFI
                     PAPPTNLCACNDCPHMKRNTLEKLYISMKYELPEINIAEPTISFARNSIRRMLDISAK
                     AGL"
     gene            complement(1473..1796)
                     /locus_tag="PLL28_13445"
     CDS             complement(1473..1796)
                     /locus_tag="PLL28_13445"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNF70379.1"
                     /translation="MAKQNIIVFNDVGSGELHSAQVTLRALTHPLRLKIISFIDKHRK
                     INVHKIYTGLNLDQSIASQQLNILRGANLVITHREGKFIYYSLNYDTIDQMNKLVEDF
                     RKNGK"
     gene            complement(1798..2133)
                     /locus_tag="PLL28_13450"
     CDS             complement(1798..2133)
                     /locus_tag="PLL28_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNF70380.1"
                     /translation="MPAIHTIKLNTDNGAEIRVDYNHLRKTVLVIRSVNHKLRQQMIK
                     LLEEYPQLTVTEIYIKMRLEQSIASQHLAILRRSGVVITKRSGKFIYYSLNQDRLNDI
                     GRLIRELAK"
     gene            complement(2225..2902)
                     /gene="tsaB"
                     /locus_tag="PLL28_13455"
     CDS             complement(2225..2902)
                     /gene="tsaB"
                     /locus_tag="PLL28_13455"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNF70381.1"
                     /translation="MARILHIETATPVCSVSIAASGITQAVLTTSSGNDHMSVITGNI
                     LAVCEQAGIGLKDIDAVAVSSGPGSYTGLRIGASTAKGICHALNKPLIAVPTLLAMVS
                     GIQEKYQEEALLFCPLMDARRMEVYTLVADRDCNISAPARAYVIEEPYFDFIPAGRKT
                     VFFGSGVEKTLNFLPEGSISDLHYFPSAEHLHLLALQQFNRQIFADTAYFEPEYGKEF
                     YTPAKKQ"
     gene            complement(2903..4570)
                     /locus_tag="PLL28_13460"
     CDS             complement(2903..4570)
                     /locus_tag="PLL28_13460"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNF70382.1"
                     /translation="MKICLPAASRRLLIPLLFISAQAVAQQEVITRFEKTISYLADDK
                     LEGRATGSAGEQLASAYIIQQFNEIGLTPAGDAGTYLQSFEARSGKKPGEENYIIAAS
                     RYTMDKLHYPHPMTGNGIASGNLIAVGFGITAPALSYDDYAGKDVKGKIVLIKTSSPD
                     GTHPHSKYIEFNDERKKIQLAAANGAAGVIFYNEDANYELPGDDYRRNVTAENIPVFI
                     VDSTTAKSLLQFTGAVELHTQLNEEVRTGHNVLGFIDNHASNTVVIGAHYDHLGHGEI
                     EGSLYRGAPAIHNGADDNASGVAMMIELARFLKQMPSSNNYLFIAFSGEEMGLFGSKA
                     FTQSPMMLQYQFNYMLNYDMVGRMDTTNAIIINGVGTSPAWATLKSISNFDMTLTTTE
                     SGIGPSDQTSFYLKDIPVVHFFTGSHADYHKPSDDAGLINYMGMLKVFKYSIALISAL
                     DDDGKLAFTKTQDSNNENTPRFKVTMGVVPDYAYSGKGMRVDGVTDGKPAQAAGVIAG
                     DIVIKLGSFDVTDMMGYMTALSKFTKGETVTLVVLRNNAPVELQVTF"
BASE COUNT         1371 a         1077 c          943 g         1411 t
ORIGIN      
        1 gtagtgagta gtgggtattg agtagtgaga ctaatgcaat cattcgctcg cgttgcgagc
       61 gaatttgcat agtgcccact caccactcac cactcaccca ctccctgtgg attagcagtt
      121 aactgactgt tagtacctat ggataacccc atgagttatc ccagcgtcaa atttccacag
      181 ccaaataatg tgcagtaccc taggtagtac caacatggta cgtacgttga cggattgtga
      241 acagacaaaa agttttcctg ttaattttgt accaacattg tgctctattt ggtggatatc
      301 cccataaatc gggaacctca actgtgtata tactgtttgt acatccaata aaactatgga
      361 aaacgctttt tccaaaaatg tgtatcaatt cttatccacc aatacacagc actgatgatg
      421 attctttata tttatataaa atataattca tgataatagg aggagagaaa gttgtggata
      481 ttgaaaaact ggagagaacc ggttttgtcg atgtggatgt agatccatct cttgatctgt
      541 ttgccgagat caataaatta aaaaaggaaa aaaatgccgt catcctggcg cattactacc
      601 aagagcctga tattcaggat attgcagatt atataggcga tagcctcggt cttgcacaac
      661 atgccgaaaa gacaaatgcg gacatgattg tgtttgccgg tgtgcatttc atggcagaaa
      721 cagctaaaat tctcaatccc gacaaaaaag tcatcatgcc tgatgctaaa gcaggatgtt
      781 cacttgccga tagttgtccg cccgcccttt tcagaaaatt caaagagcag catcccggac
      841 ataccgttat cagttatatc aattgcacag ccgaaataaa agcgctcagc gatattatct
      901 gcacaagcag taatgccgtt aagattgttg aaagcctgcc ggcggatgaa cccattattt
      961 tcgcaccgga caagaacctt ggcagatata tcgagaaaaa aacaggacgc aaaatggtgc
     1021 tgtgggaagg atcttgtatg gtacatgaaa tatttagtct cgaaaagatt accaaactca
     1081 aacaccgtca tcctaacgca aaggtgatcg cccatcccga atgtgaagaa ccagtattgg
     1141 ccatcgccga ttttattggc agtacaacag gtttacttaa atacgccact accgattctg
     1201 cccaagaata tatcgtagtc actgaaaccg gtattattca ccaaatgcaa aagagcgccc
     1261 ctcagaagac ttttattcct gctcccccaa ccaacctatg cgcatgcaac gattgtcctc
     1321 acatgaagcg caatacgctc gagaagctat atatcagcat gaagtatgag ctgccggaaa
     1381 ttaatattgc tgaaccaact atttctttcg caaggaattc aataagacgt atgctggata
     1441 tttctgcaaa agcaggattg taaaagaaaa tcttatttac cgttttttct gaaatcttcg
     1501 acaagcttat tcatctggtc gatggtatcg taattgagcg aatagtaaat gaatttaccc
     1561 tctctatgtg ttattaccag atttgctccg cgaaggatat ttaactgttg actggcaata
     1621 ctctgatcca aatttaatcc cgtataaatc ttatgtacgt tgattttgcg gtgtttatcg
     1681 ataaacgaaa tgatcttcag tctcagcgga tgtgtcaaag cacgcaaagt tacctgggcc
     1741 gaatgaagtt caccgctgcc cacatcatta aatacgatga tgttttgctt tgccatacta
     1801 ttttgccagt tcccgtatca gtcgcccaat gtcattcaaa cgatcctgat tcaaagaata
     1861 atagatgaac ttaccactgc gcttggtaat tacaacgcca ctgcggcgca atatcgccaa
     1921 atgctgcgaa gctatcgatt gttcaagtcg catcttgata tagatctccg ttaccgttaa
     1981 ttgcggatac tcctccaaca gtttaatcat ctgttgacgc aatttgtggt ttaccgacct
     2041 gataactaac accgtctttc gcaaatggtt gtaatccacg cgaatttcgg caccgttgtc
     2101 ggtgttgagc tttattgtgt gtatagcggg catagggtgt atgtttgggt cacgataccc
     2161 actctgacct tcagttagta aagctatcaa taatcattct aaaaaacaaa tcggcaacag
     2221 cttatcactg tttttttgca ggagtgtaaa attccttccc atattccggt tcgaaatatg
     2281 cagtatcagc aaaaatctgc ctgttaaatt gttgaagtgc taacaaatgc aaatgttcgg
     2341 cagagggaaa ataatgtaaa tctgatatgg atccttccgg aagaaagttt aaagtttttt
     2401 ctacacctga accgaagaaa acagttttcc ttcccgcagg aataaaatca aaataaggct
     2461 cctctataac ataggctcgt gcaggagccg atatgttaca atctctgtcg gcaaccaagg
     2521 tatacacttc catacgcctg gcatccatca agggacaaaa tagcagcgct tcttcctgat
     2581 atttttcctg tataccggaa accatggcca acaaggtagg cacggcaata agtggtttgt
     2641 ttaaagcgtg acaaataccc tttgccgttg atgcaccaat gcgcaaaccg gtataagaac
     2701 ccggcccgga gctcacagcc actgcgtcta tatcctttaa ccctattccg gcctgttcac
     2761 aaaccgctaa aatattgccc gtgattacag acatatgatc attgccggaa ctagtggtca
     2821 atacagcttg tgtaataccc gacgctgcaa tactcacact gcaaaccggt gtggctgttt
     2881 cgatatgtag aatgcgtgcc aatcagaatg ttacctgcaa ttctacaggt gcattattgc
     2941 gtaatacaac taaagtaact gtttcacctt tcgtaaattt tgacagcgct gtcatataac
     3001 ccatcatatc tgttacatca aagcttccca gtttgatcac aatatcgccg gcaatcacgc
     3061 ccgctgcttg tgcaggtttt ccatcggtaa caccatccac gcgcatgccc tttccactat
     3121 aggcataatc aggaaccaca cccatggtta ctttaaatcg cggtgtgttt tcattattgc
     3181 tgtcttgtgt tttggtgaag gcaagtttgc catcatcatc aagtgcagaa attaatgcaa
     3241 tgctgtattt aaatactttc agcatgccca tgtaattaat taaaccggca tcatcacttg
     3301 gcttgtgata atcggcatgt gatcctgtga agaaatgcac tacaggaata tcttttaaat
     3361 aaaatgacgt ctgatcactc ggcccaattc cactctctgt tgtggtgagt gtcatgtcga
     3421 aattggatat gctttttaat gttgcccacg ccggactagt accaacacca tttatgatga
     3481 tggcatttgt tgtatccata cgaccaacca tatcataatt caacatgtaa ttaaattggt
     3541 attgcaacat catcggtgat tgcgtgaacg ccttgctccc aaacaatccc atctcctctc
     3601 cgctgaatgc aataaataaa taattgtttg acgatggcat ttgtttcagg aacctcgcta
     3661 attcaatcat catcgcaaca ccgctggcat tatcgtcagc accattgtga atggcaggtg
     3721 ctccgcgata aagcgatccc tctatttcac catgtcccaa atgatcatag tgtgcaccta
     3781 ttaccaccgt atttgatgcg tgattatcta taaaccctaa cacgttgtga ccggtgcgca
     3841 cttcttcatt taattgtgtg tgtaattcaa ctgcaccggt aaattgtaat aaactttttg
     3901 cagtggtgct gtccacaata aataccggaa tattttctgc tgttacattt ctgcgataat
     3961 catctccggg taattcataa tttgcatctt cattgtaaaa tattacgcct gctgcaccat
     4021 ttgctgctgc taactgaatt ttttttcgct catcattaaa ttcaatatac ttggaatgcg
     4081 gatgtgtgcc atcgggcgaa gaagttttta ttaatacgat ttttcctttt acatccttac
     4141 cggcataatc gtcgtatgat aaagccggag cggtgatacc aaatccaact gctattaaat
     4201 ttccggaagc tattccattt cccgtcatcg gatgcggata atgtagcttg tccatggtat
     4261 atcgcgatgc ggcgatgatg taattctcct cacccggttt tttgcctgat cgcgcttcaa
     4321 aactttgtag ataggttcct gcatctcctg caggtgtcag acctatttca ttgaactgtt
     4381 gaatgatgta ggccgatgcc aattgctctc cggcacttcc tgttgcacgg ccttcgagtt
     4441 tatcgtcagc aaggtagctg atggtttttt caaatcgtgt tattacctct tgctgagcaa
     4501 cagcttgtgc gctgatgaag agaagaggta tgagcagacg acgacttgcc gccggtagac
     4561 aaattttcat aactgcaaaa ttaatgcgcc ccgagctttt ccggcgattt aattgtcacc
     4621 gggatcggtt atttcggttg aagaagcgaa ttgtatgttg cctgatcttc caaaacctct
     4681 attgcccgct gaacatcatc atcaaaacgg aagctggatt caatgcgacc ggtgccgtta
     4741 taataacgcg atgctatctc aatattgagt tcagctatta tttcatcctt gaatttatag
     4801 ag
//
LOCUS       DAOQHY010000224         4688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_10617_length_4688_cov_5.374919, whole genome shotgun sequence.
ACCESSION   DAOQHY010000224 DAOQHY010000000
VERSION     DAOQHY010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10617_length_4688_cov_5.374919"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1437
                     /locus_tag="PLL28_13465"
     CDS             <1..1437
                     /locus_tag="PLL28_13465"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HNF70383.1"
                     /translation="ISADGFQMPPLRQYPKRYAYSTNGGLSWTNNGSDYSIPSGSCGS
                     GTTFTWDLPDFTTASPVLIRIYGWNANNVNGQSRIYNGVVSGSTCALTTWYADADGDG
                     YGNAAVSTVACNAPVGYVADNTDCNDANASINPGMAEICNGIDDNCNGSIDEGLTFTT
                     YYADADGDGYGDPGTSVTSCAPVAGYVADNTDCNDASGAAYPGATEVCDGIDNDCDGD
                     IDENVVTAVMSPTGDVTTCKSDDFVFSVEPCAGCTYQWFKNDNVIVGATGTTYATTKP
                     AYYNVQVTTAAGCFDVSDYALLTTLLNPNANIYNPNGLNLCAPTPGTNILIKVGYTAT
                     NTYQWYKDGLPYTGDGATSWRIYPTETGDYYCAITGASGCYRETEVRTVINACRMADV
                     ADQGVNIYPNPASNKVTVSLSLVSSAEYADITIVNMMGQSLATTTTAMSDGMADVTIN
                     TADLPAGMYTVIVRAGDQINMNELMIVK"
     gene            1681..3816
                     /locus_tag="PLL28_13470"
     CDS             1681..3816
                     /locus_tag="PLL28_13470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HNF70384.1"
                     /translation="MLLSSPIIIQAQTNPTPQAVPYTQDFSGLAHASTTYPAGIQGWK
                     CSSAATTSFVTTGPTGDQALIASSSASNNSGGVHNYNGKIGLLASGSTNPGIVLSLNT
                     TGYSNLLLSYDIMVIRNPYDGTTNTRLNEATVQYRIGNTGAWITLTGIEYQSGTTLQT
                     GSGVTTPQNPLSRSILLPDATDNVAEVQVRWISRDMTGGGSRPSFAIDNISVQPDADG
                     DGYSVVTDCNDANALVYPGAPENCNGYDDDCDGLIDEGTMVTYFADADGDLYGDPATT
                     AVACVAPTGFVGNDEDCNDADAMINPAATEVCNGYDDDCDGLVDDDDPSAEGLPLWFE
                     DLDGDTFGNSDVFVFACNMPTGYSIDPGDCDDTNAGVNILATEICNGIDDNCDGNIDE
                     DLIFATWYADSDGDGYGDAASTFFTCDPAPVGYVMDATDCNDAAATVYPGATEICDGL
                     DNDCDGDIDEDVVSATITPAGVVSTCKGVNTTLTANAGESYTYQWFKNGNIIAGATDV
                     TFSSNKPGYYQVQVNLPEGCFALSPVTTITVSPNPNANISAPNGTSLCATVKLKASYD
                     ASYTYQWYDGAGEIAGATAYQYFPTMAGNYYCIVTNAFGCSRNTAEITVTACREENAT
                     ANNNLEVYPNPTSGQLHLNLSTTAKEGTASITIVNMLGEIVYSTVADYSNFELDQTLD
                     LTGVIPAGMYSVIVIQNNTIAESRIVIQN"
     gene            complement(3918..>4688)
                     /locus_tag="PLL28_13475"
     CDS             complement(3918..>4688)
                     /locus_tag="PLL28_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70385.1"
                     /translation="LYKAKLTGIFLCNLTQNFSQSRIYISDINELFAQSPGAFTESEL
                     LTAQWHYAQILFFSSQFDESFVIYEQLFQKINVSETMRWYVFIAEFFQNCLVTGHYDQ
                     AQQLCTDYFQKFFDDKNGSFYLSALIQCVKLLIHTSDFEKAKLKLDELNKLTTKTSSL
                     QFQFALRELTSAYYYLTGNYKEALILAEKNLKFMRSKRIHYLIPEYTFHSRLIKALIK
                     QKQRQKSFTEEEVKMMDAMQQGTMAQYGLLLKRLEKKS"
BASE COUNT         1334 a          947 c          985 g         1422 t
ORIGIN      
        1 atttcagctg acggttttca aatgccccca cttcggcaat acccgaaacg ttatgcgtat
       61 agcacgaatg gcggtttgtc atggacaaac aatggtagcg attattctat tccaagcggg
      121 tcttgtggtt ctggaactac ctttacctgg gatcttcctg attttactac agccagcccg
      181 gtattgattc gtatttatgg ctggaatgct aataacgtaa acggacagtc acgcatctat
      241 aacggtgttg taagcggttc aacttgcgcg cttactacat ggtatgccga cgctgatggc
      301 gatggatacg gtaatgctgc ggtaagtacc gtagcctgca acgctcctgt tggttatgtt
      361 gctgataaca ctgattgtaa tgatgctaat gcatctatca atcccggaat ggctgaaata
      421 tgcaacggta tcgatgacaa ctgcaatggt tctatcgatg aaggccttac attcactacc
      481 tattatgccg atgccgatgg cgatggatac ggtgatccag gaacttcagt tacttcttgt
      541 gctccggtag caggttatgt tgctgacaac actgattgta acgatgcttc aggtgctgca
      601 tacccaggag caacagaagt ttgcgatggt attgataacg attgcgatgg tgatatcgac
      661 gaaaacgtag ttactgctgt gatgtctcca actggtgatg ttacaacatg taagagcgat
      721 gattttgtat tctctgttga accatgtgca ggatgtacct atcaatggtt taagaatgat
      781 aacgttattg taggtgctac aggtacaact tatgccacta caaaacctgc ttactacaat
      841 gtgcaggtaa caactgctgc aggttgtttc gatgtatctg attatgcatt gttgactaca
      901 ttgctgaacc ctaacgcaaa catttacaac cctaatggtc tgaacctttg cgcaccaact
      961 ccgggcacca acatcctgat caaagttgga tacaccgcta caaatactta tcagtggtat
     1021 aaagatggtt tgccttacac aggcgatggc gctacatctt ggaggattta tccaacagaa
     1081 acaggcgatt actattgtgc tattacaggt gcttccggtt gttaccgtga aactgaagtg
     1141 cgcaccgtaa tcaatgcatg ccgtatggcc gatgttgccg atcaaggcgt gaacatatat
     1201 ccaaaccctg cttcaaacaa ggtaaccgtt tcgctttcat tggtaagttc agctgaatat
     1261 gctgatatca ccatcgtaaa tatgatgggt caatcacttg ctacaactac cactgcaatg
     1321 tcagatggta tggccgatgt aacgatcaat actgccgacc tgcctgcagg tatgtatact
     1381 gtaattgttc gtgccggtga ccaaattaat atgaacgaat tgatgattgt taaataatta
     1441 gaaatttata aattgagccg cccggtagac ctatcgggcg gttttttttt gcctatttta
     1501 aacctaaacc caatattgta catgttaaaa cactatgtat tcggccattt attaacatag
     1561 gtttaattct ttttcgccaa aacctacaca agtgtaccgt ttctttgcac cgtaattcat
     1621 ttaaaaaccg gctatatgaa acagttacta cgaaaatcca ttcaactttg cttgtcttta
     1681 atgctactta gtagtccaat tattattcag gcacaaacta accccacacc acaggctgtt
     1741 ccttacacac aagatttttc tggtttagca catgcatcaa ctacttatcc tgccggtatt
     1801 caaggttgga aatgtagttc tgctgctact acctcttttg ttacaaccgg cccaaccggc
     1861 gatcaggcac ttattgcctc atcaagcgct tccaataata gtggtggcgt gcacaattac
     1921 aatggtaaaa ttggattgct cgcttccggt tctacaaatc cggggatagt gctttcatta
     1981 aataccacag gttacagcaa cttgctattg agttacgata tcatggtaat tcgaaatcca
     2041 tatgatggaa ctaccaacac aagattgaat gaagcaactg ttcaatacag aattggaaat
     2101 accggcgcat ggatcacatt gacaggtatc gaatatcaaa gcggaaccac tttgcaaacc
     2161 ggttcgggtg ttacaacacc tcagaatccg ttgtcgcgtt ctattttact ccctgatgca
     2221 actgataacg ttgcagaagt tcaagttcgc tggatttccc gcgatatgac cggcggcggt
     2281 tctcgtccaa gttttgctat tgataatatc agcgttcaac ctgatgctga tggagatgga
     2341 tattcagtgg ttacggattg taacgatgct aacgcattag tatatccggg tgctcctgaa
     2401 aattgtaacg gttatgacga cgactgcgac ggtttgatcg atgaaggtac catggttacc
     2461 tattttgcag atgccgacgg cgatttatat ggcgatcctg caaccaccgc tgtagcctgc
     2521 gttgctccga caggatttgt tggcaatgat gaagattgta atgatgctga tgctatgatt
     2581 aatcccgcag ctacagaagt atgtaatggg tacgacgatg attgcgacgg actggtagat
     2641 gatgacgatc cttctgctga aggtttgcca ttgtggtttg aagatttaga tggcgataca
     2701 ttcggaaatt ctgatgtatt tgtattcgct tgtaatatgc caaccggata ctctattgat
     2761 ccgggcgatt gtgatgatac caatgccggt gtaaatattc tggcaactga aatttgtaac
     2821 ggtatagatg ataactgcga tggcaatatt gatgaagacc tcatctttgc tacttggtac
     2881 gccgattccg atggcgatgg atatggagat gcagcttcaa cgttctttac ctgcgacccg
     2941 gctcctgtag gatacgtaat ggatgcaaca gattgtaatg atgcagcagc taccgtttat
     3001 ccgggagcga cggaaatttg tgatggactc gataatgatt gcgatggtga tatcgatgaa
     3061 gacgtggtat ctgctaccat cactccggca ggtgtagttt ctacttgtaa aggagtgaat
     3121 actaccctca ctgcaaatgc aggcgaaagt tatacctatc agtggtttaa aaatggaaat
     3181 attattgccg gtgctaccga cgtaactttt tcttcaaata aacccggcta ttatcaagta
     3241 caggtaaatc ttcctgaagg atgttttgca ttgtcacctg ttaccacaat tacggtttcg
     3301 ccaaatccga atgcaaatat cagtgcacca aacggaacaa gcttatgtgc taccgttaaa
     3361 ctgaaagcca gctatgatgc ctcgtatacc taccaatggt acgatggtgc cggtgaaatt
     3421 gcaggcgcta cggcgtatca atattttcca acaatggcag gaaattatta ttgcatagtt
     3481 actaatgctt ttggttgctc acgtaataca gcagaaatta cggttaccgc atgccgcgaa
     3541 gaaaatgcaa ccgcaaataa taatctggag gtttatccaa atcctacctc aggtcaattg
     3601 catttgaatt tatctacaac agctaaggaa ggaacggcat caattacaat tgtaaatatg
     3661 cttggtgaaa tagtttattc tactgttgca gattacagca atttcgaatt agatcaaaca
     3721 cttgatctta caggtgttat tcctgccgga atgtattctg taatcgttat acaaaataat
     3781 acaatagctg aatcaagaat agtaattcag aattaaaatg cgcggttgcc ggtaataaat
     3841 actttcaacc atttcagcat acaataaaaa atggactgct tatgttaggc agtccatttt
     3901 tttatactat aaagtagtta agattttttt tcaagtcttt ttaacaataa gccatattgc
     3961 gccatagtgc cttgttgcat tgcatccatc attttcacct cttcttcagt aaaagatttc
     4021 tgacgctgct tttgcttaat aagtgctttt attaagcgtg aatggaaggt gtactcagga
     4081 atcaggtaat ggatgcgttt cgaccgcatg aattttaaat tcttctcagc taatataagt
     4141 gcctccttgt agtttccggt caaatagtaa taggcgcttg ttaattcacg caaggcaaat
     4201 tgaaattgta atgatgaagt ttttgttgtc agcttattta attcatccaa ttttaatttt
     4261 gccttttcaa aatcacttgt gtgaataagc aattttacac attgtattaa tgctgataaa
     4321 taaaagcttc cgtttttatc gtcaaagaat ttttgaaaat aatccgtgca aagttgctgt
     4381 gcctggtcat agtgtccggt taccagacag ttctggaaga attcggcaat gaatacatac
     4441 cagcgcatgg tttcagaaac attgatcttt tgaaacaatt gttcatagat cacaaagctt
     4501 tcatcaaact gactgctaaa aaataatatc tgcgcataat gccattgtgc agtcaacaat
     4561 tccgattctg taaatgctcc cggcgattgt gcaaataatt cgttgatatc gctgatgtaa
     4621 attctgctct gagagaaatt ctgcgtgaga ttgcacaaaa atattccggt gagctttgcc
     4681 ttatataa
//
LOCUS       DAOQHY010000225         4665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_10683_length_4665_cov_4.545119, whole genome shotgun sequence.
ACCESSION   DAOQHY010000225 DAOQHY010000000
VERSION     DAOQHY010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10683_length_4665_cov_4.545119"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1567)
                     /locus_tag="PLL28_13480"
     CDS             complement(<1..1567)
                     /locus_tag="PLL28_13480"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNF70386.1"
                     /translation="MKNLRAYVVIALMGSVLFIPFLGASHLFDWDEINFAEASREMIV
                     TGNYTQTQIDFHQFWEKPPFFFWLQSASMHIFGVNEFAARFPNAICGILTLMILYAIG
                     KRLRDERLAGWWVICYAGSMLPQFYFKSGIIDPWFNLFIFLGVYGWIRYVQDNKQLKW
                     IVFSGIFAGLSMLTKGPVGVGLIGISVGIWYLFTRFRKFPNLWHILVFLICVAAPMTP
                     WVAIEISHNGTWFIKEFFTYQTRLATTEDASHGGFLLYHFVVILFLCFPASAFAIGNI
                     VKKNNFEQEHADFRKWMIILLCVVLVVFTIVQTKIAHYSSMAYFPLTFLAAQRLANID
                     KRWSSVEIVLIAVTGFVFSVSLFAIPFVLQRPELLETYILDPFSKEALRADAHWQGWE
                     PVMGMLMLGCCLFTVIWAMRSRGSAAITWLFLSSMVIINISINTITPHIERYSQGAMI
                     DFLDEKSREDVYTDVIGLKSYAQLFYGKRTPEDVNNPRFKAWAARTYPDVAEFDAIKW
                     RSAYGKWLREGDID"
     gene            1624..3774
                     /gene="rnr"
                     /locus_tag="PLL28_13485"
     CDS             1624..3774
                     /gene="rnr"
                     /locus_tag="PLL28_13485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNF70387.1"
                     /translation="MSKKKKKKSPKTQRAGLHFEDELFDFISGKLAKGASLKVIFKWA
                     SKTGEMDGVFDAIENLVNAGKIEEFAPDLFRSKQQKGGGATMVGIVDMTQSGNAYVVI
                     DGHLYDVFVPSHKTQRAFNGDTVRVRITSMGKNKKPEGEITEIIKREKELFVGTVDIN
                     GQHSFAVTDDRFNNYDFYLPAEEVKKHKLTHGDRIIMRIKDWPPGMKSPIGQVVTKLG
                     APGDHASDMEAILVETGIIYAFPKEVEKEADAIPVTIPEKVIASRLDIRDRFTITIDP
                     YDAKDFDDAISYRKLDNGNLEIGVHIADVSYYVTSGSAIDKEAYARGTSVYLVDRVLP
                     MLPEKLSNNVCSLRPHEDKLCFSAIFEMDEEGNLIEEWFGKTVIHSDRRFTYEEVQEV
                     IETGKGDHVEEIMILNKVTHALRKKKFAAGAISFETQEVKFILDENFVPTGLYVKERK
                     EAHMLIEDLMLLANRRVATFLSKEHSRVPFVYRVHDRPDMQRLEEFSMNARRFGYKLK
                     LDSPKNISAELNRLMVEIKGKPEQNILENLAIRCMAKAVYTTKNIGHYGLAFDYYTHF
                     TSPIRRYPDLMVHRVLEDVLDKSPLTYPKIDDLEDHCRHCSATERKAMEAERESVKYK
                     QVEYLSKHVGEEFDGVISGITPYGIFVELIANKCEGMVHVDEIRDELMFDESRRRLVS
                     LTHSKDYELGDNIRIKVKKADLAHRRLDFTPVIQ"
     gene            complement(3755..4423)
                     /locus_tag="PLL28_13490"
     CDS             complement(3755..4423)
                     /locus_tag="PLL28_13490"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNF70388.1"
                     /translation="MQPTIIFEDSQLIVLNKPTGIAVEADRFGHPGVQGWVAAYFDGR
                     KMPSNTIIGIVHRIDRPVSGVVLMAKKKSVLVDLNAQFAAGTVSKVYYAGVEGTPPKP
                     EGKLEHFLMKDPEQKKAIITPKKSKRSAAVSLSYKTLHSDKGITLLEVRPHTGKYHQI
                     RAQLSEMGCPIIGDALYGSKKSFLQDAILLHAASLTCIHPATKEEVTFSAPLPNAWPI
                     VLPV"
BASE COUNT         1447 a          962 c          999 g         1257 t
ORIGIN      
        1 tatcaatatc tccttcgcgc agccatttac cgtacgcact acgccatttt atagcatcaa
       61 attccgctac atcaggataa gtgcgtgcag cccaggcttt aaatcgcgga ttatttacat
      121 cttccggcgt gcgcttaccg tagaataatt gcgcataact ttttaatcct atcacgtcag
      181 tgtaaacgtc ttccctactc ttctcatcaa gaaaatctat cattgcacct tgcgaataac
      241 gttctatatg cggggtgatg gtgttaatac tgatatttat aatcaccata ctgcttaaaa
      301 atagccatgt aattgcagca cttccgcgtg aacgcatcgc ccagattaca gtgaataaac
      361 aacatccgag catgagcata cccattaccg gctcccaacc ctgccaatga gcatctgctc
      421 gaagggcctc tttagaaaag ggatccaaaa tatatgtctc taataattca ggtcgttgca
      481 atacaaatgg aatagcaaat aatgatacac tgaaaacaaa tcctgttact gcgattagaa
      541 caatctccac gctgctccat cgcttatcta tatttgccaa tcgctgagcc gcaagaaatg
      601 taagcgggaa ataagccata gaagaataat gtgcaatttt tgtctgcaca atggtaaata
      661 cgacaagcac tacacataat aaaataatca tccattttct aaaatcagca tgctcctgtt
      721 caaaattgtt tttcttgacg atattgccaa ttgcaaatgc acttgcagga aagcagagaa
      781 ataaaatcac aacaaagtga tataataaaa atccgccgtg actggcatct tcagtagttg
      841 cgagtcgcgt ttgataggta aaaaattctt tgataaacca ggttccgtta tgcgaaattt
      901 ctatcgccac ccaaggtgtc atcggcgcag ccacacatat taaaaataca agaatatgcc
      961 agagattcgg gaattttctg aatcgagtaa aaagatacca tataccaact gagataccaa
     1021 tcaacccgac gccgacaggt ccttttgtga gcatcgataa gcctgcaaat attccgctga
     1081 atactatcca ttttaattgc ttgttgtcct gcacatatct tatccaccca tacacaccga
     1141 ggaaaatgaa taaattaaac caggggtcga tgatgcctga tttaaaataa aactgcggca
     1201 gcatacttcc cgcgtaacaa atcacccacc atcccgccaa tcgttcatcg cgcaggcgct
     1261 tgccgattgc atacaaaatc atcaaggtga gaataccgca aatggcattg ggaaatcgcg
     1321 ctgcgaattc gttaacgcca aatatgtgca tggatgccga ctgcaaccag aagaaaaatg
     1381 gcggtttttc ccaaaattga tgaaaatcga tttgtgtctg cgtgtaattg ccggtcacta
     1441 tcatttcacg tgaggcctcg gcgaagttga tttcatccca atcaaacaga tgcgaagctc
     1501 ccagaaaagg gatgaacaaa acactaccca taagggctat caccacataa gcacgcaggt
     1561 tcttcatatt caggcggtgg aaaattacaa aaaggttatc gttcataccc gtatctttag
     1621 gggatgtcga agaaaaagaa gaagaaaagt cccaaaacgc agcgtgccgg actacatttt
     1681 gaagatgagc tttttgattt catctccggt aagttagcca aaggagcctc actgaaagtg
     1741 atattcaaat gggcctctaa aaccggggaa atggatggtg tgttcgacgc aatagagaat
     1801 ttggtgaacg ccggaaaaat tgaagagttt gcacctgacc tctttcgctc caaacaacaa
     1861 aaaggtggcg gcgctaccat ggtgggtatc gtagatatga cgcaaagcgg aaatgcgtat
     1921 gtggtgatcg atggacattt atatgatgta tttgttcctt cccataaaac acagcgcgca
     1981 tttaatggcg acactgttcg cgttcgtatc acctcgatgg gaaaaaataa aaaacccgaa
     2041 ggtgaaataa cagaaataat taaacgagaa aaagaattat ttgtaggcac ggtagatatc
     2101 aatggtcaac attcatttgc tgttactgac gacagattta ataattatga tttttatctt
     2161 cctgccgaag aagtaaaaaa acataaactc acccatggcg atcgcattat tatgcgcatt
     2221 aaagactggc ctccgggaat gaaaagtcct atcggacaag tagtaaccaa actaggcgct
     2281 cccggcgatc atgcgagcga tatggaagcg attttggttg aaacaggaat tatttatgct
     2341 tttccaaaag aagttgaaaa agaagctgac gctataccgg tgacaattcc tgaaaaagtg
     2401 atcgcctctc gcctcgacat tcgcgatcgt tttacaatta ccatagatcc ctatgatgcg
     2461 aaagattttg atgatgccat ttcatatcgc aagctcgata atggcaattt agaaatcggt
     2521 gtacatattg cagatgtttc ctattatgtg acttcaggct cagccattga taaggaagca
     2581 tacgcaagag gaacatctgt ataccttgta gatcgtgtgt tgccgatgtt gcctgagaaa
     2641 ttgtcgaata atgtatgctc tctgcgcccg catgaagata aattatgctt cagcgcaatt
     2701 ttcgaaatgg atgaggaggg aaatttaatt gaagaatggt ttggcaaaac cgtaatccat
     2761 agtgatagaa gatttactta tgaagaggtt caggaagtca ttgaaaccgg caaaggtgat
     2821 catgttgaag aaataatgat cctgaataag gtgacgcatg cattgcgcaa aaagaaattc
     2881 gctgcaggtg ccatatcatt cgaaactcag gaggtcaaat ttattttaga tgaaaatttt
     2941 gtgcctaccg gattatatgt aaaggagcga aaagaggcac acatgctcat tgaagatttg
     3001 atgctgctcg ccaatcgtcg tgttgccacc ttcctttcca aagaacacag tcgcgtgcct
     3061 tttgtttatc gcgttcatga tcgtcccgat atgcagcgat tagaagaatt ttctatgaat
     3121 gcccgtcggt tcggttataa actgaaatta gattcgccga aaaatatttc tgctgagtta
     3181 aacagactga tggtagaaat aaaaggtaaa ccggagcaaa atattctcga gaatctcgcc
     3241 atccgatgca tggcaaaggc ggtgtatacc acaaaaaata tcggtcacta cggacttgca
     3301 tttgattatt atacgcactt cacctctcct attcgtcgct accccgattt aatggtgcac
     3361 agggtgttgg aggatgtgtt ggataaatcg ccactcacct atccgaaaat cgacgacctg
     3421 gaagatcact gtcgccattg ttcagccacc gaacgcaagg ccatggaagc agagagagaa
     3481 agtgtgaaat acaaacaagt agaatactta agtaagcatg ttggcgaaga atttgatggc
     3541 gttatcagcg gcatcacacc ttatggtatt ttcgttgaac tgattgccaa taaatgcgag
     3601 ggcatggtgc atgttgatga gatcagagat gaactcatgt ttgatgaatc tcgccggcga
     3661 ttagtgagtc ttacccattc aaaagattat gagcttggcg ataatattcg catcaaagtg
     3721 aaaaaagctg atctcgcaca tcgcagattg gattttacac cggtaataca ataggccagg
     3781 cattcggaag tggcgcagaa aaagtcactt cttcttttgt tgccggatgt atacaagtta
     3841 gcgaagctgc gtgcaacaat attgcatcct gcaaaaatga tttctttgaa ccatacagtg
     3901 catctccaat aatgggacaa cccatttcac ttaattgtgc acgtatttga tgatattttc
     3961 cggtatgtgg tcgcacttcg aggagtgtaa tgcctttgtc ggaatgcaat gttttatatg
     4021 acaaagaaac tgctgcactg cgcttacttt ttttgggggt gatgatggct tttttctgtt
     4081 cgggatcttt cattaaaaaa tgttcaagct tgccctcagg ttttggaggt gtaccttcca
     4141 ctccggcata atacacttta gacacggtgc ctgcagcaaa ttgcgcatta agatccacca
     4201 gcaccgattt tttcttcgcc atcagtacaa caccgctaac gggtctgtca atgcgatgca
     4261 cgatgccaat gatggtattc gacggcattt tcctgccatc aaaataagca gccacccacc
     4321 cttgcacacc cggatgccca aaacgatcgg cttcaacggc aattcccgtg ggtttgttta
     4381 atacaatcaa ttgcgaatct tcaaatatga tagtcggctg catgaggcaa aggtggtgaa
     4441 taatttgaga atttgagaat tagctgatta gcaaatggga tggtgcatgc atggagattt
     4501 ttatcatagt tagcaagttt gagggttagc aagttagcaa gtgaggttaa ttagcaaatt
     4561 tgggaattag caaattagca agttaggaag ttagcaagtc agcaagtgtg atgggctttt
     4621 aacgctggcg cgttaatagg gaatgcaatt atattgcttt gcaat
//
LOCUS       DAOQHY010000226         4640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_10769_length_4640_cov_3.466521, whole genome shotgun sequence.
ACCESSION   DAOQHY010000226 DAOQHY010000000
VERSION     DAOQHY010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10769_length_4640_cov_3.466521"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PLL28_13495"
     CDS             <1..561
                     /locus_tag="PLL28_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70389.1"
                     /translation="YTKKDFTIVQGDTIFKKYEKYAFEFSNGDTILLIESYSIKSDWT
                     LSRYIARGDDEEQVGWQQKFDLKGNITFEQFCDTSTGDCNRQVQYSYYPNGNLMARVC
                     FYKHKYDGSSFFYYNDGSFKYHLEYKNGLLWNAHATYDQQGNITDQGDFCDGTGTLNV
                     YAANGVLLKQRIYVRGKLKRTIQIAK"
     gene            complement(568..1332)
                     /locus_tag="PLL28_13500"
     CDS             complement(568..1332)
                     /locus_tag="PLL28_13500"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNF70390.1"
                     /translation="MATSGKKTNNIIKQYGLTVGDKLFTVFVYLESRSDCRASIGQKG
                     IHIRLSKYMSEHDRFDQEMELLDWAKKYIAKNGWHERPPLYRKYHDGDTLKVMGKEFT
                     IRIRYVSNKNASNGNLKNGVIQLQLSEGMSPKQEQKHLSYLVSRLIGNEFQPAIAQRV
                     QVLNDIHFKKPIKKLRLRDNISNWGSCSWDGSINISVRLLFAPPEVIDYILIHELAHL
                     VEHNHSDRFWAQVEKAMPGYKLAEDWLHKHGDQCIF"
     gene            complement(1481..2167)
                     /locus_tag="PLL28_13505"
     CDS             complement(1481..2167)
                     /locus_tag="PLL28_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70391.1"
                     /translation="MSAVKSSIIALLFMCSYTLQAQISVEVSESVSAMSLGDRTGYAV
                     TIPRSTEKNAAKAFRSWIIEKQKKADINESAKHELRVTNFAYGPLGSAPVNVYFQFNE
                     GKDGVIVVGYFEVNGTFVSTATNPDAVAGCKQFMTHFAYRMEKLAIAENLDAEKKALE
                     KRNDEQAGLEKQQKALNDKIADCEETIAKAKDDLKTNAGDQETKQQEIKYQSKKVSEV
                     EDALKVYDNY"
     gene            2337..3725
                     /locus_tag="PLL28_13510"
     CDS             2337..3725
                     /locus_tag="PLL28_13510"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HNF70392.1"
                     /translation="MMKKLLTIALVCTTLISARAQSEETSFSATGRGGTATSFVTDYQ
                     AIGINPANLGFETDNAFSLGVGEFGYGLFSNALVKDDVRAILFNNEDSLSAEDQVTLA
                     RSFLNEGITMNVDVMPIGFSLRLPKLGTFGFSMKGNMAYHTLFAGQAANIVFEGYNYE
                     EYIDTIIFDGTTIYGVAYEPLSLGELFDDTEISLNITSEFNVAYGRKLFGSTEGVSVY
                     GGFGYKYILGLAYLDINSTDGVLSGTAALGLDIMDLQESETPFTITSNPYEPVGRGNG
                     FDVGLSLKIGEGLTFGASVVDMGKMTYTANVLQINDFTLDTIAFSGLTSTDPIQLISD
                     ILGDPELIAYSGIQSFTVNLPTKLRLGGSVKISDMLNVGVDAVFPMNDVAGAYTSPVI
                     GLGGEFTVLKVIKLSTGLSAGGGYSYNIPAGIGFDFKIWEIGFATRDILTWFGESSPT
                     VSFAAGVLRFKL"
     gene            complement(3813..>4640)
                     /locus_tag="PLL28_13515"
     CDS             complement(3813..>4640)
                     /locus_tag="PLL28_13515"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNF70393.1"
                     /translation="QKKEKNYLFLPRFRHLSAHGGIQVSGWAVYPIFEGKMKLFAYLV
                     AMAMLAGCSVHDSEPKSAVPAPPLPHSQAPLAARMKTAGLVNVQEHIPDILVELKYST
                     TDNFVKTDVYGDLDSAYLQPDVVNMLEKAYTHLQTLDSSLTFIVYDATRPLSVQQYMW
                     DILQIPIEEKGKYLSNPDKGSIHNYGAAIDISLAVKTTGKALDMGTTYDFFGPEAEPI
                     QEAIMLANGKLTQQQHQNRLTLRKAMESAGFRQLPTEWWHYNACSREEAKKRYDVVE"
BASE COUNT         1173 a          940 c         1097 g         1430 t
ORIGIN      
        1 tataccaaaa aagacttcac tatcgtgcag ggtgacacca tattcaagaa atatgaaaag
       61 tatgcatttg aattttctaa tggcgatact attttattga ttgaatcata cagcattaaa
      121 tccgactgga cactcagcag atatattgcg cgtggtgatg atgaggaaca agtgggttgg
      181 cagcagaagt ttgatttaaa aggcaatatt acttttgagc aattttgtga tacgagtacg
      241 ggagattgca atcgtcaggt gcagtatagt tactacccta acggcaacct gatggcgagg
      301 gtttgttttt ataagcataa gtatgatggc agcagttttt tttattacaa cgatggttca
      361 tttaaatatc atcttgaata taaaaatggg ttgttatgga atgcacatgc tacctatgat
      421 caacagggaa atattaccga tcagggcgac ttttgcgatg gaaccggcac cttgaatgtg
      481 tatgctgcaa atggcgtgct gttaaagcag cgtatttacg tgcgtggaaa gctgaagcgc
      541 acgattcaga ttgcgaaatg atctacctta aaatatgcac tggtcgccgt gcttatgcag
      601 ccagtcctcg gcgagtttat acccgggcat agctttttct acctgtgccc agaatctgtc
      661 ggaatgattg tgttccacca aatgtgcgag ctcgtggata aggatataat cgatcacctc
      721 gggaggcgca aataacaagc gaacggagat attgatactg ccatcccaag agcaacttcc
      781 ccaattggag atattatcgc gcagtcgcaa ttttttgatc ggttttttga aatgaatatc
      841 gttcaatacc tgcacgcgtt gtgcaatggc aggctgaaat tcattaccta taagtcggct
      901 tactaaatag ctcaaatgtt tctgttcctg ctttggcgac atgccctcac tcaattgtaa
      961 ttgaatgacg ccattcttta aatttccgtt acttgcattt ttattggata catagcgtat
     1021 gcgaatggtg aattcttttc ccattacttt aagcgtatct ccgtcatggt atttgcggta
     1081 taacggcggg cgttcatgcc aaccgttttt tgcgatatac ttttttgccc aatcaagcaa
     1141 ttccatttct tgatcgaacc tgtcgtgttc cgacatgtat ttggaaagtc ggatgtgaat
     1201 acctttttgt cctatgctcg cgcgacaatc gctgcgtgat tcgaggtaga caaacacggt
     1261 gaacaatttg tcgccaacgg taagcccata ttgcttaatg atattgttcg ttttctttcc
     1321 gctcgtcgcc atgtgccagc tacaacattg aggatggtaa ttccggttcc attaggagat
     1381 ccaaaaataa taaaacaggt taaatcacca taaacaaccc ataaggtgtt tacgcatgag
     1441 ctaacctgtt tccattgaag taaggtaaag aaaagccggc ttagtagtta tcgtaaacct
     1501 tcaaagcatc ttcaacttcc gatacctttt ttgattgata ttttatttcc tgttgttttg
     1561 tttcctggtc gccggcattt gttttcaggt catcctttgc cttggcgatc gtttcttcac
     1621 agtctgctat cttgtcgttc agtgcttttt gctgtttttc cagtccggct tgttcatcat
     1681 tgcgtttttc cagcgctttt ttctcggcat ccagattttc ggcaatggct aatttttcca
     1741 tgcgatacgc gaaatgcgtc atgaattgtt tacatccggc aaccgcatcg gggtttgtgg
     1801 ctgttgaaac aaaagtgcca ttcacttcaa aatatccaac cacgatcacc ccgtctttgc
     1861 cttcgttgaa ttgaaaatat acatttacgg gagcggagcc taacgggcca tatgcgaaat
     1921 tggtaacacg caactcatgc ttggcagatt cattgatatc cgcttttttc tgcttttcga
     1981 tgatccaact gcggaatgcc tttgcagcat ttttctctgt gctgcgcggg atggtgacag
     2041 catatccggt gcggtcgccg aggctcatgg cggatacgga ttcactcact tcaactgata
     2101 tctgtgcctg taaagtgtag ctgcacatga acaggagtgc aatgatgctt gatttaactg
     2161 ctgacatagg atagtttttg atgttgttta cgcgccatat gcaaaaagta ttccaatttg
     2221 cgaatggcaa ttttgataag gaatatgctc taatatacac ctgaaagatc aaattgtcgt
     2281 tatgtggata gaccactgca acactttgtg tatttttgaa ggcataagct ctgaacatga
     2341 tgaaaaaatt gctgaccatc gcacttgttt gcacaacact tatttccgca cgagcacaat
     2401 cggaggaaac ttcattcagc gctacggggc gaggaggaac ggcaacttct tttgttaccg
     2461 attatcaggc tatcggtatc aatcctgcca accttggttt tgaaacagac aacgccttct
     2521 ccttaggcgt tggtgaattt ggatatgggc tgttttcaaa tgcgttggtc aaggatgatg
     2581 tgcgtgctat tttatttaat aatgaagaca gtctttctgc tgaagatcag gtaacacttg
     2641 ccagatcatt cttaaatgaa ggtattacca tgaacgtgga tgtcatgccg atagggttta
     2701 gtttgcgact acctaaatta ggtaccttcg gattttccat gaaaggcaat atggcatatc
     2761 atacgttgtt tgccggccag gcagcgaata ttgtttttga aggatataat tatgaagaat
     2821 atatcgacac tatcattttc gatggtacaa ccatttatgg tgttgcgtat gaaccgttga
     2881 gcctcggcga attatttgat gacacagaaa tttcattgaa tatcacttct gaatttaatg
     2941 ttgcctatgg tagaaaatta tttggcagca cagaaggtgt gagcgtgtac ggtggctttg
     3001 gctataaata tattttagga cttgcctatc tcgatatcaa ttcaacagat ggtgtgctga
     3061 gtggaacggc agcattggga ttggatatca tggatctgca ggaaagtgaa acgcccttta
     3121 ccatcaccag caatccatac gagccggttg gacgaggtaa tgggtttgat gtaggtctca
     3181 gtttaaagat cggtgaaggc ttgacctttg gtgccagtgt agttgatatg ggtaagatga
     3241 catacacggc taacgtgctg caaataaatg atttcacgct cgatacaatt gcattcagcg
     3301 gactgaccag caccgatcca attcaactca tctccgatat tcttggagat cctgaattga
     3361 tcgcatattc aggcatacaa agtttcactg ttaatctgcc aacaaaattg cgactcggag
     3421 gttctgtaaa aatttcagac atgttgaatg ttggtgttga tgctgttttt ccgatgaatg
     3481 atgtagcagg tgcttatact tctccggtga tcggattagg aggggaattt actgtattga
     3541 aggtgatcaa attgtcaacc ggactttccg ccggtggggg ttattcttat aatattcctg
     3601 ccggtatcgg gtttgatttt aaaatttggg agatcggctt tgccacccgc gatatcctca
     3661 cctggttcgg cgaaagcagc ccgacggtgt cgtttgcagc gggggttttg aggtttaaat
     3721 tgtgataacg tatttttttt cacgcagagg cgcagagtcg cagagttgca tttattcccc
     3781 tgcgtctctg cgcctctgcg tgaaaaattt aactactcca ccacatcata acgttttttc
     3841 gcttcttcac gggagcaggc attataatgc caccactctg taggcagttg cctgaagcct
     3901 gctgattcca tggctttgcg gagggtgagc cggttttggt gttgttgttg ggtgagtttg
     3961 ccgtttgcca gcatgatcgc ttcttggatg ggttcggcct ccgggccaaa aaaatcgtag
     4021 gtggtaccca tgtcgagggc tttgccggtt gtttttacag caagtgagat atcgatagcg
     4081 gcaccataat tatggataga ccctttgtca gggttggaga ggtatttccc tttctcttct
     4141 atcggtattt ggaggatgtc ccacatgtat tgctgaacgc tcaaaggtct ggtggcatca
     4201 tatacgataa aggtgaggct gctgtcgagg gtttggaggt gggtgtaggc cttttcaagc
     4261 atgttcacta catcaggttg aaggtaggcg gagtcaagat ctccgtagac atcggttttc
     4321 acgaaattgt ctgtggtgct gtatttcagc tctaccagga tgtcggggat atgttcttgc
     4381 acattgacga gcccggcggt tttcattcgc gctgccagcg gtgcctggct atgcgggagt
     4441 gggggtgcgg gaacggcaga cttcggctca ctgtcatgta cagagcagcc cgcaagcatt
     4501 gccattgcga ccaggtaagc aaaaagcttc attttcccct caaaaatagg gtaaacagcc
     4561 catcctgaca cctgaatgcc accatgggca gataaatgcc taaaacgagg taaaaacagg
     4621 tagttttttt cttttttttg
//
LOCUS       DAOQHY010000227         4475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_11386_length_4475_cov_5.523529, whole genome shotgun sequence.
ACCESSION   DAOQHY010000227 DAOQHY010000000
VERSION     DAOQHY010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11386_length_4475_cov_5.523529"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PLL28_13520"
     CDS             <1..305
                     /locus_tag="PLL28_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70394.1"
                     /translation="LTNVRMVSGVANFIYREGKPARMPESDISTIKSLLNAEFHTEVH
                     PDSFAIGTQVKINFGPMAGTIGELVAIENEKLFLLRIDVIQQALLIKVPAAYLEKI"
     gene            complement(314..778)
                     /locus_tag="PLL28_13525"
     CDS             complement(314..778)
                     /locus_tag="PLL28_13525"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HNF70395.1"
                     /translation="MRLLAGIAFCLMCASCASTQELQYKGFDDFTVTNILKEPKVQMD
                     LNLYNPNPIGGTIRNMEINIAVDQKTLGSVSLDDKVRIKKQAEFTMPISFTTSVSEMG
                     GILSAGLKSFVGQDEMPLGISGEITIQKFLVFRKTFSFEYKDGIDIHDIIQK"
     gene            complement(883..2706)
                     /locus_tag="PLL28_13530"
     CDS             complement(883..2706)
                     /locus_tag="PLL28_13530"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF70396.1"
                     /translation="MPTSGRLNRKQIEAEISGLLHTSWDLVSKRNSEIAELSSKALAL
                     AEQINDNNLKGLCILEGALYQCLVKSDYHLSIKMSDEGYAMMKGEFKKRYAPYYHLNV
                     GRNYHFMGDHVSAQHNYLECVKCIESMSEQNQFELKWLAHAYYNLYILFNFSGAEFAQ
                     AEYLQKALTIYKQIGDVGGEGNCYNSYAVYYFRLNNYTQAYENLQRAYALAEQEQATS
                     LLSIYSANIGLVKVKMGEVETGFTFFDKAQHYDLELDSPYHHAHTQNQMGEAFIILER
                     YEEAIERFAKALQYFESIGVKRSMSNTYEQMAKAYAGLGNHERAYHYQQAYAQSLKDV
                     FSDEKTFAVAKARNDFELEMRDQEARKLRLKNKQIEQYARQLEMSNDELRQFAHVASH
                     DLREPLRMVSSYVQLLQRSMDIPLTGDQQEFMRYILQGTHTMQNLISDLLALAAISNV
                     RNSELVDLNIIVQQVKSNLQPVIEERNAVIKSTLLPAVIADETHMLQLFLNLISNGIK
                     YNTSTLPLIEISFATSGRYYQFSVADNGIGIPAEYKEKIFLIFQRLHSKEEYSGTGIG
                     LAICKKIVDQAGGTIKVSSRKGGGSIFTFTLPVIEHQPESV"
     gene            2824..>4475
                     /gene="htpG"
                     /locus_tag="PLL28_13535"
     CDS             2824..>4475
                     /gene="htpG"
                     /locus_tag="PLL28_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNF70397.1"
                     /translation="MSIETGNIAVQTENIFPIIKKFLYSDHEIFLRELVANAVDATQK
                     LRKLSSMGEIGGELGDTNIRISIDKEAGTLTISDRGIGMSGDEVKRYINQVALSSASE
                     FLDKYKGLEDGKSIIGHFGLGFYSAFMVADQVALVTRSAREGHDAVRWTCDGSPQFTL
                     EVVEKETRGTDVILYINEENKEFLEDNRIQTLLDKYCKFLPVPIQFGESVTKEKDEAG
                     NETEVTAPRIINNPNPAWTKKPAELTDEDYTNFYRELYPYAEVPMFWIHLNVDYPFNL
                     TGILYFPRIKKSYEIQKNKIHLYSNQVFVTDEVNAVVPEWLTLLHGVIDSPDIPLNVS
                     RSYLQSDQNVKKINSYITKKVAEKLEELFKKDRAAFEEKWENINVIIKYGMLSDEKFF
                     ERGEKFCLLQNTEKKFFTLDEYKAHIAPAQEDKNKQVIALYTNNVTDHHVYVDAAINK
                     GYDVLEIDNVIDNHFISFIEQKMTGVQFKRVDADTIDQLIDKDEKIESVLSDTEQTSV
                     KELFEQVVDANAGTVVLTPMSPNDQPVTITKPEFMRRMKEMS"
BASE COUNT         1357 a          946 c          889 g         1283 t
ORIGIN      
        1 aactcaccaa tgtacgcatg gttagcggtg tggcaaactt tatttatcgc gaaggtaaac
       61 ctgctcgcat gccggaatcg gatatttcaa caataaaatc gctgttgaat gccgagtttc
      121 ataccgaggt gcatcctgat tcatttgcaa taggcacaca ggtaaaaatc aacttcggcc
      181 ccatggcagg caccattggc gaattagttg ctatagaaaa cgaaaaatta ttcctgctga
      241 gaattgatgt gatacagcaa gcattactca tcaaagtacc cgcagcatac ctggaaaaaa
      301 tataatggaa tttttatttc tgaatgatgt cgtgaatatc aatgccatcc ttgtattcaa
      361 atgaaaatgt ctttctgaat accagaaatt tctgaatggt aatctctcct gatatcccca
      421 aaggcatttc atcctgacca acaaacgatt ttaatcctgc agacagtatt ccgcccattt
      481 cactgacgct tgtcgtgaaa gatataggca tggtgaactc tgcttgtttt tttatgcgca
      541 ccttatcatc taaagagaca cttccaagcg ttttttgatc tacggcaata tttatttcca
      601 tattgcgaat ggtgccacct atcggattgg gattatacag attcaggtcc atttgcactt
      661 ttggttcctt gagaatattc gtaaccgtaa aatcatcaaa tcccttgtat tgcaattcct
      721 gtgtcgatgc gcaggatgca cacattaaac aaaaggctat accggctaga agtctcatgt
      781 acctaatctt tcaggcttta ttacaaaaaa tatgcccgat tttagttttg cgtgataaaa
      841 tggcttactt gcaaagataa ataaggccgg gaattaaagc gattatacac tttccggctg
      901 atgttcaatc accggcaatg taaacgtaaa tatgctgcca ccacccttgc gcgacgatac
      961 cttaatggtg ccgccggcct gatcaacaat ttttttacaa attgccagtc cgataccggt
     1021 gccgctatat tcctctttgg agtggagtcg ttggaagatg agaaagattt tctccttgta
     1081 ttctgcaggt atgccaatgc cattatcggc aacggagaat tgataatatc tgcccgaagt
     1141 agcaaatgaa atttcaatca gcggtagagt gctggtatta tattttatac cattggaaat
     1201 cagattgagg aacaattgca acatatgtgt ttcatctgct attactgccg gaagcaaggt
     1261 gctttttatt actgcatttc tttcctctat taccggttga aggtttgatt tcacctgttg
     1321 cacaataata ttcagatcaa ctaactccga attgcgtacg ttactgatgg cggctaaggc
     1381 caacagatca ctgattagat tttgcattgt atgagtgccc tgcaaaatat atctcatgaa
     1441 ttcctgctgg tcaccggtca aaggtatatc cattgagcgc tggagcaatt gtacgtatga
     1501 cgataccatg cgcaatggtt cacgtaggtc gtgactggca acgtgtgcaa attgtcgcaa
     1561 ttcatcattc gacatttcca actgccttgc gtattgctct atctgtttat ttttcagcct
     1621 taattttctt gcttcctgat cacgcatttc caattcaaaa tcattgcgag ctttggcaac
     1681 ggcaaatgtt ttctcatcac tgaatacatc ttttaatgac tgagcatagg cttgttgata
     1741 gtggtatgcg cgctcatgat ttcccagtcc ggcatacgct ttggccatct gctcgtaagt
     1801 attactcatg gagcgtttta cgccaatgct ttcgaaatat tgcaatgcct tggcgaaacg
     1861 ttcgatagcc tcttcataac gttccaaaat aataaatgcc tcacccattt gattttgagt
     1921 atgggcgtga tgatagggac tatctaattc caaatcgtaa tgctgcgcct tatcgaaaaa
     1981 agtaaatcct gtttctactt cacccatttt cacttttacc aagccaatat ttgcagaata
     2041 aatagaaagg agcgaagtag cttgttcctg ttctgccaat gcatatgcgc gctgaagatt
     2101 ttcatatgct tgcgtataat tatttaatct gaaataataa actgcatatg aattgtaaca
     2161 attcccttca ccaccaacat cgccgatctg cttataaatg gttaatgcct tttgcaaata
     2221 ctctgcttgt gcaaattcgg caccggagaa attgaacagg atatataaat tgtagtaggc
     2281 atgagctagc cattttaatt caaattgatt ttgttcgctc attgactcta tacatttcac
     2341 acattccaga taattatgtt gtgccgaaac atgatcgccc atgaaatggt aattgcgtcc
     2401 aacattcaga tgataatatg gagcatatct ttttttaaac tctcctttca tcatcgcata
     2461 accctcatca gacattttga tgctcaagtg ataatcactt ttcaccaaac actgataaag
     2521 ggctccttcc agaatacaca aacccttcag attattatcg ttgatttgct cggcaagagc
     2581 gagtgccttc gacgacaatt ccgctatctc agaattgcgc ttgctcacca ggtcccaaga
     2641 ggtgtgtaac agtccgctga tctctgcttc aatctgtttg cggttgagtc gacctgaggt
     2701 aggcatgccc aaatataagg gtttgagcaa tttcaggaga tgacatgcat cggcagcgac
     2761 aaaatgtcag caatatcccc atggcacagg ctttgagatg ccgaaagcac aaacaataac
     2821 gctatgtcaa tagaaacagg aaatatcgcc gtccagactg agaatatctt ccccattatc
     2881 aaaaaattcc tttacagcga ccatgagatc tttcttcggg aattggttgc caatgcggta
     2941 gatgcgactc aaaagctccg caaattgagt tctatgggtg agatcggcgg agaactggga
     3001 gacactaata tccgtatttc aatagataaa gaagccggaa ccctcaccat ctctgacaga
     3061 ggaattggta tgagcggcga tgaagtgaaa cgctatatca accaggtggc tttgtccagt
     3121 gcctctgaat tcctggataa atacaaagga ctggaagatg gtaaatccat catcggacat
     3181 tttggccttg gtttctatag cgctttcatg gttgccgacc aggtagcatt ggtaactcgc
     3241 tcggcaagag aaggacatga tgctgtgcgc tggacttgcg atggcagtcc gcaattcacc
     3301 ctcgaagttg ttgaaaaaga aacgcgtgga actgatgtga ttttatacat caacgaagaa
     3361 aacaaagaat tcctcgaaga taatcgcata cagactctgc tcgataaata ttgtaagttt
     3421 ttgcctgtac ccattcaatt tggtgaatct gttaccaagg aaaaagatga ggccggaaat
     3481 gaaacagaag taactgctcc acgcattatc aataacccaa atccggcgtg gaccaaaaaa
     3541 cctgctgagc ttaccgacga ggattatacc aatttctatc gcgaattata tccgtatgcc
     3601 gaggtgccga tgttttggat acatttgaat gtagattatc cattcaacct cacaggtatt
     3661 ctgtatttcc cacgcattaa aaaaagctat gaaatacaga aaaataaaat ccacctgtac
     3721 agcaatcagg tatttgttac cgatgaagta aatgctgttg tccctgaatg gctcaccttg
     3781 ttgcatgggg tgatcgactc gccggatatt ccgcttaacg tatcgcgaag ctatctgcag
     3841 agtgatcaaa acgtaaaaaa aataaatagc tacatcacca aaaaagtagc cgaaaaactc
     3901 gaagagctat ttaaaaaaga cagagcagca ttcgaagaaa aatgggagaa catcaacgtg
     3961 atcatcaaat atggtatgct cagcgatgag aagttttttg aacgtggtga aaaattctgt
     4021 ctgctgcaaa atactgaaaa gaaattcttt accctcgatg aatacaaagc acatattgct
     4081 cctgctcaag aggataaaaa caagcaggtg attgcacttt ataccaataa tgtaaccgat
     4141 catcatgtgt acgttgacgc agcaatcaac aaaggatatg atgtgttgga aatcgataat
     4201 gtgatagaca atcacttcat ttctttcatc gaacaaaaaa tgaccggcgt acaattcaaa
     4261 cgcgttgacg ccgatactat tgatcaactt atcgataaag atgaaaaaat tgaatctgtt
     4321 ttaagcgata ccgaacaaac cagtgtaaaa gaattgttcg aacaagttgt cgatgccaat
     4381 gccggcactg ttgtgctcac accaatgtcg ccgaatgatc aacccgtaac tataaccaaa
     4441 ccggaattca tgcgtcgtat gaaagagatg agtca
//
LOCUS       DAOQHY010000228         4466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_11422_length_4466_cov_6.211517, whole genome shotgun sequence.
ACCESSION   DAOQHY010000228 DAOQHY010000000
VERSION     DAOQHY010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11422_length_4466_cov_6.211517"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PLL28_13540"
     CDS             <1..795
                     /locus_tag="PLL28_13540"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNF70398.1"
                     /translation="HTDHKLSHGIRGEIGTRNAPALFNLAWKKSFMWDGAIHQLDVQA
                     LAPINNVIEMDMSTADLVSRLQKSAFYRKLFFQAFNDSVITGQHLLQALGAFQVTLIS
                     DHSKYDSVQLGTAQFTEQEAAGYQLFKQYCNSCHTEPLFTNEGFANNGLAPDPQLHDA
                     GRYRITGNPSDSLKFAIPSLRNLSYTYPYMHDGRFAQLRQVLDHYTSEKYFSATLDPS
                     LNHAFPLSSNDKVDMIAFLLTLDDKQFVFNKAYQFPPELLPNSKTR"
     gene            801..2174
                     /locus_tag="PLL28_13545"
     CDS             801..2174
                     /locus_tag="PLL28_13545"
                     /inference="COORDINATES: protein motif:HMM:NF025600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HNF70399.1"
                     /translation="MKKFIALYAIIMFMATVVKAQLSPAITSWLQNNTETGSYYVEGN
                     YTPIDNGILYNCQTVEYSTDYVYVHTKGIPSYPTGPFMDGNPSIAEDQDIIFKIPLHP
                     QQNTGTPTPTTPGNIGVFINGVALFDYRDGVAWNTTTSALCGGPGNPPCPGGPGAIMD
                     WNRDAVPAEKIGFDCSKGHPAMGNYHHHQNPSAFKLDLNVISTVCNLYDADGLYMIDS
                     NAHSPLIGFAYDGYPIYGAYGYKNADGTGGIVRIKSSYQLRSITERTHAADGSDVDDG
                     PDVSVTYPLGYFREDYEFIAHPGEQDYLDDHNGRFCVTPEYPEGTYAYFATVDENWNS
                     AYPYVVGPSFYGVYANRAVTSVTEATTVYTPTVAISDNNFDNMHIGIYPNPTADLLII
                     QAAGITSGDLIITLYDLNGRQLAQTQIAQGQTITYFDTKTLYAGNYIVHITNGNSSQT
                     EQVTIVK"
     gene            2282..3814
                     /locus_tag="PLL28_13550"
     CDS             2282..3814
                     /locus_tag="PLL28_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70400.1"
                     /translation="MAVSHCYTLIHALTMSEKRQFVAFSERHVVGEEKKYLALFHILE
                     SMPTYNEHQVQKAMREKGLNTKWFKGDQNYLYNQLLEHLAQFHNGKSGNLQVKAGLMH
                     IEILFDKGLFRQCRKEIEKAMALARMLEFFELQIQILFWERKIALATSEKTSRTIAQI
                     LDDLEQATAMIAIGNQYLLLYEKAESLRQRVIKTRKPADLAAFRKLLKDPLLGMRSHH
                     PTKQSQIRFHQIHAMWAYLHKAKDDELAHNYAIIKLFDRSPEYRNEYPLDYIAIYSRI
                     LSILKLDDAKVFHAQLTNFRAFEPDIHSIGYRNIRAQIFAQSYMIELSAYLTQGNFSD
                     AMRTVRAIEASMPEHAPFISRGNSLSFQFMISYALFANGEYKSARSALNQLLNEYDDK
                     VRPDLYNFARLLSLMTHLELGNYSNIRSQASSIAYYFRKHGKLYATENKILNYFNNPE
                     HYKGRRQLHYLELIEAQLEKIRSASIEKYALGYIDFFTWIASRRERVPMSTIAGRRNK
                     RG"
     gene            complement(3815..4306)
                     /locus_tag="PLL28_13555"
     CDS             complement(3815..4306)
                     /locus_tag="PLL28_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNF70401.1"
                     /translation="MRPISFTAELKIIGVNPYVDVPEDILQKIFMAAKKDKGFIPVSG
                     KVNNVPYRQTLVRFKGEWRLYINTSMLPRSPKRIGEVLKLSVAYDPADRSIALHPKLA
                     AALEDNLAAKKVFDKMSPSHQKEIIRYINSLKSEASVERNVARSIQFMLGKERFVGRD
                     PEK"
BASE COUNT         1305 a          954 c          967 g         1240 t
ORIGIN      
        1 cataccgatc ataaattaag tcatggcata cgaggtgaaa tcggcacgag aaatgcacct
       61 gcgttattta atcttgcatg gaaaaaatct tttatgtggg atggcgccat ccatcaactt
      121 gatgtgcagg cactggcgcc gatcaataat gtaattgaaa tggatatgtc aacagcagat
      181 cttgtttcac gattacaaaa aagtgctttt tatagaaaat tattttttca ggcttttaac
      241 gattctgtca tcaccggtca gcatttatta caagctttgg gtgcttttca ggtaaccttg
      301 atttccgatc attcgaaata tgatagtgtg caacttggca cagcgcagtt taccgaacag
      361 gaagcggcag gatatcaatt gttcaaacaa tattgcaaca gctgtcatac ggaaccactt
      421 tttaccaatg aagggtttgc caacaacggg cttgcgccgg atccacaatt acatgatgcc
      481 ggtcgttatc ggataacagg caatccgagc gatagcctga aatttgccat tcccagcctt
      541 cgcaatctca gctacactta tccatacatg catgatggca gattcgcaca gctcaggcaa
      601 gtgctagatc actatacctc ggaaaagtat ttttcagcta ctttggatcc gtcgttaaac
      661 catgcgtttc cgctttcgtc aaacgacaag gttgatatga tagcgttctt actgaccctt
      721 gacgacaagc aatttgtttt caataaagcc tatcaatttc cacctgaatt attacccaat
      781 tccaaaaccc gataattcca atgaaaaaat tcattgcatt gtatgccatt attatgttca
      841 tggcaacagt agtaaaagca caattaagcc ctgccattac ttcctggttg caaaataata
      901 ctgaaaccgg atcatattat gtagaaggca attacacacc tatagacaat ggcattttat
      961 ataattgtca gacggtggaa tattctaccg attatgtgta tgtgcacaca aaaggcatcc
     1021 catcctaccc taccggacca ttcatggatg gcaatccttc gatagctgaa gatcaggata
     1081 tcattttcaa gataccactg catccacaac aaaacacggg cacaccaaca ccaacaacac
     1141 ccggcaacat tggcgtgttc atcaatggtg ttgccttgtt cgattatcgc gatggcgttg
     1201 catggaatac gaccacatca gcactatgcg gtggtccggg aaatccgcct tgtcctggag
     1261 gtcccggtgc tatcatggac tggaatcgcg acgcagtgcc tgcagagaag ataggatttg
     1321 attgcagcaa ggggcatcct gcgatgggaa attatcatca ccatcaaaat ccatcagcat
     1381 ttaaattaga cctgaatgtg atctcgacag tttgtaattt atatgatgca gatggtttat
     1441 atatgatcga cagcaatgca cattcgccat tgataggatt tgcatatgat ggttatccca
     1501 tctatggagc ttatggttat aaaaatgcgg atggtacagg tgggatcgtg cgcatcaaat
     1561 caagttacca attacgcagt attacagaac gcacacatgc cgccgatggt agtgacgttg
     1621 atgatggtcc ggatgtaagt gttacatatc ccttaggata ttttcgtgag gactatgaat
     1681 tcattgctca tccgggtgaa caagattatc ttgatgatca caacggtcgt ttttgtgtaa
     1741 cacccgaata tcctgagggc acttatgcat attttgctac tgttgatgag aattggaatt
     1801 ccgcttatcc atatgttgta gggccaagct tttatggtgt gtatgcgaac agagccgtta
     1861 cttcagttac ggaggcaacg acagtttata cgccaacagt tgccatcagc gataataatt
     1921 ttgacaatat gcatatcggc atttatccaa atccgacagc agatttactc ataatacaag
     1981 ctgcaggtat tacttccggc gatttgatta ttacgctcta tgatttaaat ggcagacagt
     2041 tggcacaaac gcaaatagct caagggcaaa cgataaccta ttttgataca aagacattgt
     2101 atgcaggaaa ttatatcgtg cacatcacca atggcaacag cagccaaact gaacaggtga
     2161 cgattgtaaa gtaggaatgt ttcatgataa gcttacaaat gctcatcgga ctgcaacata
     2221 gttgtctggt gagcatttgt agttttttgc ctatttttag gaagtctgat ttagcccttt
     2281 tatggcagta agtcattgtt atacattgat acatgcactc accatgtcag agaagcgaca
     2341 atttgtagct ttctcggaac ggcatgttgt cggggaggag aaaaagtatt tagcgttatt
     2401 ccatattttg gaatccatgc caacctacaa tgaacatcaa gttcagaagg ccatgcgcga
     2461 gaaagggctc aataccaaat ggttcaaagg cgatcagaat tatttataca atcaattgct
     2521 cgagcacctc gctcaatttc acaatggcaa atcgggcaac ctgcaggtga aagcaggatt
     2581 gatgcatatc gagatattgt tcgacaaagg attgtttcgt cagtgtcgga aggagatcga
     2641 gaaggcaatg gcgctagcac ggatgttgga attttttgaa ttgcaaatcc aaatactttt
     2701 ttgggaacgc aaaattgcat tggctacttc agaaaaaaca agcagaacaa ttgctcagat
     2761 actcgacgat ttggagcagg ctactgcgat gatagccatt ggtaatcagt atctcctatt
     2821 atatgaaaaa gccgaatcac tgcgtcagcg tgtcatcaag accagaaaac ctgctgatct
     2881 tgcagcgttc agaaaattac tgaaagaccc tttgctcgga atgcggtctc atcacccaac
     2941 aaaacaatca cagatccgct ttcatcagat ccatgccatg tgggcatatc tgcacaaggc
     3001 caaggatgat gaactcgcac ataattatgc tattatcaaa ttatttgatc gatcgccgga
     3061 atacaggaat gaatatccgc tggattatat cgctatctac tcgcgcatac tgtcgatctt
     3121 gaagcttgat gatgcgaagg tattccatgc acaactcaca aattttcgcg cttttgagcc
     3181 cgatatacat agtatcggat atcgaaacat tcgcgctcaa atattcgcgc aatcatatat
     3241 gatcgaatta tcggcgtatc tgacgcaagg aaatttttct gatgccatgc gcactgtaag
     3301 agcgatcgaa gcatccatgc ctgagcatgc tccatttatc agcagaggca acagtttatc
     3361 gtttcagttt atgatcagct atgcactgtt tgccaatggc gaatacaaat ccgcgcgaag
     3421 tgcattgaat cagttgctca acgaatatga cgacaaggtg cgaccggact tgtataattt
     3481 cgcccggtta ttaagtctga tgacgcattt ggagctgggc aattattcca atatccgctc
     3541 gcaggcatcc tccatagcct actatttcag gaaacatggt aagctgtatg ccactgaaaa
     3601 taaaatattg aactacttca acaatccgga gcattataag ggtagaaggc aattacacta
     3661 tttagaattg atagaagcac aattagaaaa aatacgcagt gcttcgatag aaaaatatgc
     3721 gttgggatat atcgatttct tcacttggat cgcatcgcgc agggagagag taccgatgtc
     3781 gaccatagcg ggaaggcgca acaaacgcgg ttgatcattt ttccggatcg cgacctacaa
     3841 aacgctcttt gccgagcatg aattggatcg aacgcgcaac atttcgttca acacttgctt
     3901 cgcttttcaa agaattgata tagcgtatga tctctttttg atgggaagga ctcattttat
     3961 caaacacctt tttcgctgct aaattgtctt cgagcgctgc agctaatttt ggatgaagtg
     4021 caatagatct gtcagcagga tcgtacgcga cactcagttt taatacttca ccaattcgtt
     4081 taggtgaccg gggaagcatg gaggtattga tatacaatcg ccactcacct ttgaacctga
     4141 ctagcgtttg acggtatgga acattattca ccttgccgga gacgggtatg aatcctttgt
     4201 ctttttttgc tgccatgaat attttttgga ggatatcttc cggcacatca acatatgggt
     4261 tcacgccaat aatcttgagt tcagcggtga atgaaatcgg gcgcatgatc caaaggtaat
     4321 gcgtttatgt gttcatcccg aggggtcggg acttcgctgc gctgcgtgtg cgaaatgcac
     4381 tgattaacct gtattggctg cacacccgcc tgcggcgagg caggcttgca catggagcgc
     4441 agcgacatcc cgcctaagcg ggactt
//
LOCUS       DAOQHY010000229         4459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_11460_length_4459_cov_5.194142, whole genome shotgun sequence.
ACCESSION   DAOQHY010000229 DAOQHY010000000
VERSION     DAOQHY010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11460_length_4459_cov_5.194142"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1778)
                     /locus_tag="PLL28_13560"
     CDS             complement(<1..1778)
                     /locus_tag="PLL28_13560"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HNF70402.1"
                     /translation="MSIIKTPDQRVRVFISSTINELAEERIAARSAIESLKLIPVFFE
                     AGARPHPPRDLYSAYLSQSHIFLGIYWNSYGWIAPGAEISGLEDEYRLCGKTKPKLIY
                     VKNSTERQEKLDLLLQDISNSDSACYQKFNTADELKQLIENDLSVLMSEIFESSLISG
                     QGSIDSSTGAIENKHDHPIIDIPLIKSEMIGRERDLQLVSELLLREDTSLITILGAGG
                     TGKTTLATFIGQANKDKFKDGVLFIQLAAITNSKLVVNTIGEKLGIQDNGKHDLISLI
                     IDLLTGKQFLLILDNFEQVVDASVVVSQLINQCKDLKVLVTSRSILHIRNERIYHLAT
                     LNFPVEESAIAFADLQNYAATKLFLDRAQEVNQNIENSKENAEAILEICQRMDGLPLA
                     IELAAARTRFFQPAALLGRITKTLDLVNKGHKDLPERQQTLRGAIEWSYNLLSPDTQS
                     IFRQMAVFKRSWTMEAADAILVINESAVDAEEYTERLLDVSLIKTDILNKELEPRFNM
                     LQTVHEYAGEMLAQDPDCEKVKLQYAKYFLQLCEDAEPYLWNRNGAIWLDRIESEYQN
                     IRASFYIFMELGLPEMAWRFFPCLAP"
     gene            complement(1966..2751)
                     /gene="dapF"
                     /locus_tag="PLL28_13565"
     CDS             complement(1966..2751)
                     /gene="dapF"
                     /locus_tag="PLL28_13565"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNF70403.1"
                     /translation="MPIQFYKYQGTGNDFVLFDNRSGQIDRTRYDWFNFICDRRFGIG
                     ADGVMLLQEREGYDFEMLYFNADGLPGSMCGNGGRCMVQFAKRLGIDKAQYHFLASDG
                     DHDAIITGDEVSLHMRDVLSVELHKGQYILNTGSPHVVLAVDDVMAVDVNRDGAQLRY
                     DPLFAPGGTNVNYIHIQGETMHIRTYERGVEQETLSCGTGVTASALVSALMQNLPFGT
                     HEVQIRTMGGALRVQFHYHADGSFTDIYLIGPGTFVFSGEINA"
     gene            2874..4319
                     /locus_tag="PLL28_13570"
     CDS             2874..4319
                     /locus_tag="PLL28_13570"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNF70404.1"
                     /translation="MRAKILGMTMMVAVISSLATVVVYDRFIADYGHNENGSNSPYAF
                     TKASFPVAPSGMAPVDFTYSAALTTPTVVHVKTTFKQQANYGGSNDIIRQFFGDDFNW
                     EQPSAQASGSGVIMSEDGYIVTNYHVVKNADDIEVVFFDKHSAKAELVATDPSTDIAL
                     LKVDLDGLPAIRFGDSDSVRVGDWVMAVGNPFDLASTVTAGIVSAKGRKIDILGENAS
                     TPVESFIQTDAAVNPGNSGGALVNLQGELIGINTAIATPTGTFAGYSFAVPVNIVKKV
                     VKDLKAYGVVQRAYLGVEVDMTREDVGGVYVSNVVKGSGADESGIKEGDVITHINSSA
                     INSFPELQEQLSKYGPGEKVNVSLVRGNDKKNVVVLLKNQKGNTELIKKESNETLSSL
                     GIEAEEISAREAAYYQVDGGVKVTEIKNGKVFQQTRIREGFVITAIDDKKIVNYNDLA
                     KILNDKKGNNVIIEGFYPSFPNKIYNYGLSL"
BASE COUNT         1317 a         1007 c          899 g         1236 t
ORIGIN      
        1 tatggcgcta aacaagggaa aaaccgccaa gccatttcag gcaagcccaa ttccataaaa
       61 atataaaatg aagcgcgaat attctgatat tcactttcaa ttcggtcgag ccaaattgca
      121 ccgtttcgat tccataaata gggttcagca tcttcacata attggaggaa atacttggca
      181 tactgcaatt taaccttttc gcaatcggga tcttgtgcca gcatttcccc ggcatattca
      241 tgaaccgtct gtaacatgtt gaatctcggt tccaactctt tattcaagat gtccgttttt
      301 atcaaactta catctaaaag tcgctcagta tattcttcgg catcaaccgc actttcattt
      361 attacaagta tagcatcagc agcctccatg gtccaactgc gcttaaatac agccatttgt
      421 ctgaaaatac tttgggtatc cggcgacaat aaattataac tccattcaat tgcccccctt
      481 aaagtttgct gacgctccgg taaatcctta tgtcctttat ttacaaggtc tagtgttttt
      541 gtaattcttc caagcaaagc agctggttga aaaaatcttg ttcttgcagc agctagttca
      601 attgcaagtg gcaatccatc cattcgctga caaatttcaa gtattgcttc cgcattttct
      661 tttgagtttt ctatattttg atttacttcc tgtgccctat ctaaaaacaa tttcgttgca
      721 gcataatttt gcaaatccgc aaatgctatg gcagactctt caacagggaa atttaatgtt
      781 gccaggtggt aaattctttc atttctgata tgtaaaatac tgcggctagt taccaatacc
      841 ttcaaatcct tacattgatt gatgagctgg ctcacaacca ccgaagcgtc taccacctgc
      901 tcgaaattat caagaataag caaaaattgc ttgccggtga gtaagtcaat gatcaatgaa
      961 attaaatcat gtttgccatt atcctgtata cctaattttt cgcctatggt gtttacaacc
     1021 aatttagaat ttgtgatggc tgccaattgt atgaataaaa ccccatcttt aaatttatcc
     1081 ttgtttgcct gaccaataaa cgttgcaagt gttgttttac ccgttccacc tgcacccaga
     1141 attgtaataa gactcgtgtc ctctcttagc aacaattcag aaactaattg caaatctcgt
     1201 tcacgcccta tcatttccga tttaataagc gggatatcaa ttatcggatg atcatgctta
     1261 ttttctatag cacctgttga tgaatcaata cttccctgcc ccgaaatgag tgatgattca
     1321 aatatctcag acatcagtac cgatagatca ttttctatca gttgtttgag ctcatctgca
     1381 gtattaaatt tctgataaca ggccgagtca ctattcgaaa tatcttgcaa taaaaggtct
     1441 agcttttcct gacgctctgt tgaattctta acatatatca gtttaggctt cgttttcccg
     1501 caaagtcgat attcatcctc caatcccgaa atctcagctc cgggagctat ccaaccataa
     1561 ctattccaat aaattcccag aaatatatga ctctggctta aataggctga atacaaatcc
     1621 ctcggaggat gcggacgcgc accggcttcg aagaaaacag gaataagttt caagctttca
     1681 attgcactcc tggcagctat gcgttcctct gctaactcgt tgatggttga acttataaat
     1741 accctgacgc gctgatcagg agtctttatt attgacatat gatggtgtgt tttcgcatat
     1801 aaatccgatc cttacggcta cgtcaataaa gttaggcaat tcaatcctaa tgtctagcat
     1861 tcgaactcga ttaaatttaa gcaaatcacc cggtttcatg ccggttgata tctcaacccg
     1921 attagcaccg gaacgggtca acagtgaaga atgaacttct ttgctttaag catttatctc
     1981 cccactaaaa acaaaagttc cgggtccgat caggtagata tcggtgaagc tgccgtcggc
     2041 atgatagtgg aattgcacac gcagcgctcc tcccattgtg cggatttgaa cctcatgtgt
     2101 gccgaaagga agattttgca tcaaagcaga aaccagtgca gatgctgtta cacctgttcc
     2161 gcaactcaag gtctcctgtt ccacaccgcg ttcataggtg cgaatatgca ttgtttcacc
     2221 ctgaatatgt atataattta cattcgtgcc tcccggtgca aataaagggt cgtatcgcaa
     2281 ttgtgctccg tcgcggttaa catcaacagc cattacatca tcaacagcca acaccacatg
     2341 cggagatcct gtgtttaaaa tatactgtcc tttatgcagt tcaactgaca acacgtcacg
     2401 catatgcagc gacacttcgt cgcccgtaat gatggcatca tgatcgccgt cagaagcaag
     2461 aaaatgatat tgtgctttgt cgataccgag gcgttttgca aactgtacca tacatcgccc
     2521 cccatttccg cacatagagc ccggaagccc gtcggcattg aaatacaaca tctcgaaatc
     2581 ataaccctcg cgctcctgca gcaacatcac cccatccgca ccaatgccaa atcgcctgtc
     2641 gcaaataaaa ttaaaccaat cgtaacgtgt cctgtctatc tgtccactgc gattatcaaa
     2701 tagtacaaaa tcattgcctg ttccctgata tttgtaaaac tgaatgggca taaattgcgt
     2761 taataaacga taatgaggtt aaaatttgtt aagcgaaaat acaacttaat atcattcgtc
     2821 gtttagagca tgaaatttga tggagaacaa tcagaaaaaa aataaagtgc attatgagag
     2881 ctaaaattct cgggatgaca atgatggtgg cggtgataag ttcattggct accgtggtgg
     2941 tgtacgatcg gtttattgcc gactacggcc acaatgaaaa tggcagtaat tcgccatatg
     3001 cgtttaccaa ggcttcattc cctgtagcgc cgtcgggaat ggcacctgtt gattttacgt
     3061 attcggcagc gctcacaaca ccaactgttg tgcacgtgaa aaccaccttc aagcagcaag
     3121 caaattacgg tggctccaac gacatcatac gtcagttctt tggcgatgat ttcaactggg
     3181 agcaaccaag tgcccaagct tcaggatccg gcgtgatcat gtcggaagat ggttatatcg
     3241 tgacgaatta ccacgttgtc aagaatgccg acgatatcga agtggtgttc ttcgacaagc
     3301 actcggcgaa ggctgagctg gtagcaaccg atccaagcac ggatatcgcc ctcctgaagg
     3361 tcgatctgga tggattgcct gccatcaggt tcggcgactc cgactcagtg cgcgtgggcg
     3421 attgggtaat ggcagtgggt aaccccttcg accttgccag caccgtaact gcaggtatcg
     3481 tgagcgccaa ggggcgtaaa atcgatatcc tgggtgaaaa tgccagcact ccggttgaat
     3541 cgtttattca gacagacgct gccgtaaatc cgggtaacag cggcggtgcc ctcgtaaacc
     3601 tgcaaggcga actgataggc atcaataccg ccatcgccac acctacaggc acattcgcag
     3661 gctattcttt tgccgtgccg gtgaatattg ttaaaaaagt agtgaaagac ctcaaagctt
     3721 acggtgtagt gcaacgcgca tatttgggtg ttgaagtaga tatgacacgc gaagatgttg
     3781 gcggagtgta tgtaagcaat gtggtaaaag gatcaggtgc cgatgaaagc ggaattaaag
     3841 aaggtgatgt aatcacacat atcaatagtt cagctatcaa ctcattcccc gaattgcagg
     3901 aacaactgag taaatatggc cccggtgaaa aagtaaatgt aagcctcgtg cgtggcaacg
     3961 ataagaagaa tgttgtcgtc ttattaaaga atcagaaagg caacactgag ctgatcaaaa
     4021 aagaatcaaa tgaaacatta tcaagcttag gcattgaagc tgaggaaatt agcgcacgcg
     4081 aagcagcata ttatcaggtt gatggcggcg taaaagtcac cgaaatcaag aatggcaaag
     4141 tgtttcaaca aacacgcatc cgcgaaggat tcgtaattac agcaatagat gataaaaaaa
     4201 ttgtaaatta taacgatttg gcaaagattt tgaacgataa aaagggaaac aacgttataa
     4261 ttgaaggatt ttatccatca ttcccgaaca aaatctataa ttacgggtta agtttataaa
     4321 gaaataattg aagatatagg gtaattgggt tttcaaatgt gagctccgcc aaggcctctc
     4381 cggcacagcg gggctttttt tttcaattag ccaattagca aattagccga ttagccaatg
     4441 gccccccctc tctctctgc
//
LOCUS       DAOQHY010000230         4385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_11781_length_4385_cov_4.523095, whole genome shotgun sequence.
ACCESSION   DAOQHY010000230 DAOQHY010000000
VERSION     DAOQHY010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11781_length_4385_cov_4.523095"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1106)
                     /gene="lpxK"
                     /locus_tag="PLL28_13575"
     CDS             complement(57..1106)
                     /gene="lpxK"
                     /locus_tag="PLL28_13575"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNF70405.1"
                     /translation="MRWLLLPFMCLYGLVTGLRNFFFTKGWLRSFEFDFPVILVGNLS
                     AGGTGKTPHIEYLIRLLKSSYKVATLSRGYKRTYTGFGIATELSRVEDIGDEPKQYKQ
                     KFPDIEVAVSIDRVGGIYQVLHDEPQTQVILLDDGFQHRRLRAGLNIVLTTFQKPFYD
                     DYLLPVGRLRETRAGKNRAQVIIVTKCPFDITVEQQQEIMKKIGPQNDRQVFFTTLKY
                     EALYPLFADQDISLEITKPVMVTGIAGNKDMFAHVSTMYTDITKMSFADHHYYSVSDI
                     KRIKERGDTIITTEKDAMRLMEQKEAILANGLKVFVLPVEVVFINKADVFEKLVLDFI
                     VQSTPSPAETTISNE"
     gene            1432..1821
                     /locus_tag="PLL28_13580"
     CDS             1432..1821
                     /locus_tag="PLL28_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70406.1"
                     /translation="MNRIKNYPPDPMHFAISAGLKIMVVPCFYWLAVFSATLFEKPVL
                     VKWLLLDDSVIMQALLYPVMIMLPLITLLINIKARYERRIVDAVRSPFANRVQFGVMG
                     MALLSLIGVFTYTFFERLDFWMYITGK"
     gene            1818..2339
                     /locus_tag="PLL28_13585"
     CDS             1818..2339
                     /locus_tag="PLL28_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70407.1"
                     /translation="MKNYPPFGFVFRIWLYTNLLGSLLTGLYFALSESISNDFFSSIL
                     GTFFLFIALALFVGLWRSLPALLLTLGFVTFIVPYFKKTKPAFIATIAFIELLCPLTF
                     YIYFFRNRPEDLFENLLFTGIIGAYMLCILLMTIKHRKHFFTPLSLSDDPVNSEQSTS
                     EEILINSNQSDHV"
     gene            2332..2547
                     /locus_tag="PLL28_13590"
     CDS             2332..2547
                     /locus_tag="PLL28_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70408.1"
                     /translation="MFRKYGWFFIPASIVGVGIAGAALIFIVQTFLVIDSQSHSASDT
                     LYGWYPYAVTTFLAYQWIAGNLKDRKS"
     gene            2627..>4385
                     /locus_tag="PLL28_13595"
     CDS             2627..>4385
                     /locus_tag="PLL28_13595"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNF70409.1"
                     /translation="MQGWSLLMNLSTPFKEPVIIFSIVLAIILLSPILLKTLRIPSIV
                     VMIIAGVIIGPHGFQLLERDGAIVLFGTVGLLYIMFLAGLEIDLHGFRKNISRSVSFG
                     LFTFIIPFIIGFAVCRMILHLDMIPALITASMFSTQTLVAYPIVSRLGITRSAPVAIT
                     VGGTIITDTLVLLLFSVVTNFAMDKSDSFFWLRLAISVIAFAAIVLYIYPIFAKWFFS
                     KLENEGGSQFIFVLTLVFIAAFLSQLAGLEHIIGAFLAGISLTRLIPHSSTLKNRIVF
                     VGNNIFIPFFLINVGMIIDLHVLIENGGALTVAAILVTVALSTKYIAAWITQKTFGLT
                     RNEGSLIFGLSTAHAAATLAIILVGFDLGLLDEKILNGTIIVILVSCLVSSFVTENAG
                     RKYVLLTANKVDTDPEVMERILVPVGNPDSAEKLLDLAMMIRNPGSKEPIYPLAVVED
                     GPQAREQLIRARRNLQLAAEHVSSSETKVQVLTRVDVSIAGGISGAVKELGITEVVLG
                     WHSRVSTSDRLFGSIIQQVIKSTDQMLWIGRLSQPLNTIDRIAILMPPNAEFESGFFR
                     WLEGVKQLSQQTGARCMFWG"
BASE COUNT         1185 a          886 c          956 g         1358 t
ORIGIN      
        1 caatctatcc aataaagagt ccataattga aaatatttac aaagataagg gttcgtttat
       61 tcgtttgata ttgttgtttc tgcaggggaa ggcgtgcttt gaacaataaa atctaacact
      121 aatttttcaa atacatctgc cttgttaata aaaactacct caacaggcag cacaaatacc
      181 ttgaggccgt ttgcaagaat ggcttctttt tgctccatca gacgcatggc atccttttcg
      241 gtagtaatga tggtatcgcc gcgttctttg atacgtttga tgtcggaaac gctgtaataa
      301 tgatgatcgg caaacgacat ttttgtgata tcggtataca tggttgaaac atgtgcaaac
      361 atgtctttat ttccggctat tcccgtcacc attacaggtt ttgttatctc caacgatatg
      421 tcctgatcag caaataaagg atacagcgcc tcgtatttca aggtggtgaa aaatacctgc
      481 ctgtcatttt ggggtccaat cttcttcatt atttcttgct gttgttcaac cgtaatatcg
      541 aacgggcatt tggtaacgat gatgacttgt gcccggtttt ttcctgcgcg tgtttcgcga
      601 agtcggccta caggcaataa ataatcatcg taaaatggct tttggaaggt ggtgagtaca
      661 atattgagac ctgcacgaag gcggcgatgc tgaaaaccat cgtccagaag tatcacctgc
      721 gtttgaggct catcgtgtaa aacctggtaa ataccgccaa ccctatcgat gctcacagcc
      781 acctcaatat ccgggaattt ctgcttgtat tgcttgggtt catcaccgat gtcctctacc
      841 ctgcttaatt ccgtggcgat gccaaagccg gtataggtgc gtttgtatcc gcgactgagg
      901 gttgccacct tgtacgacga tttcagtaac cggatgagat attcgatatg tggtgttttt
      961 cctgtgccgc cggcgcttaa gttgcccacc aatattacag ggaaatcaaa ttcaaaactc
     1021 cgcagccacc ctttggtaaa gaagaaattt cgcaagcctg tcaccaatcc atacaagcac
     1081 ataaatggca ataataacca acgcatgggg cgaaaatagc aaaaagggtg catgggaagc
     1141 gaaaaagtcc ccctataggg acccgagggc ccctacgagg gaaacgaaca cacaagctac
     1201 tttttgggtc aatcgaccta catacgatac cacacctctt ccggtttgag ttgttaacaa
     1261 aatttatttg gatggtattg cgttttagat ttactttgca tttcaaagta atttgaataa
     1321 taatgataac aaatatcttt ttgctgattt tggcagagtt tggtcttcgt gggggacacg
     1381 agaggcaggg gttgaaatcg gcagaggggc ttaaaacaac aaaaaaggac tatgaacagg
     1441 atcaaaaact atccgcccga ccccatgcac ttcgctataa gcgcaggatt gaagattatg
     1501 gtggtacctt gtttttactg gctggctgta ttctcggcca cattatttga gaaaccggtg
     1561 ttagtgaagt ggctgttgct tgatgacagc gtaatcatgc aggcattgtt gtatcccgtt
     1621 atgatcatgc ttccgctcat taccctgttg atcaatatta aagccagata tgagcggcgc
     1681 attgtcgatg cagttcgttc cccatttgct aacagggtgc aatttggcgt aatgggaatg
     1741 gcgttgctca gtctgattgg tgtatttacc tatacatttt tcgagcgtct tgatttttgg
     1801 atgtacatca caggcaaatg aagaattatc cgcccttcgg ttttgttttc agaatttggt
     1861 tgtataccaa tttgttaggc tcgcttttaa caggattata ttttgcacta agcgaatcaa
     1921 tctccaacga ttttttcagc agtattttag gtactttctt cctgtttatt gcccttgcat
     1981 tatttgtcgg gctatggcga agtttgcctg cattgttgct gacgctaggg tttgtaacat
     2041 ttattgtgcc ttattttaaa aagacaaaac cggcatttat cgcgacaatt gcattcattg
     2101 aattgctttg tccgcttaca ttctacattt atttcttccg caacaggccg gaagatttat
     2161 ttgaaaattt actttttaca ggaataattg gagcctacat gttatgcatt ctgctgatga
     2221 ccattaaaca tcgaaaacat ttttttacac ctctgtcgtt aagtgacgac cctgtaaata
     2281 gcgagcaatc aacttcagaa gaaattttaa tcaattcaaa tcaatcggat catgtttaga
     2341 aagtatggat ggttttttat tccggcatca atagtaggtg taggaattgc aggtgcggca
     2401 ttgatattca tcgtacaaac ctttttggtg atcgatagtc agtcgcattc agcctccgac
     2461 accttatatg gctggtatcc gtatgccgtg accacctttt tggcttatca atggatagcg
     2521 ggcaatttaa aggacaggaa gtcctaaata aactttacat ccctttacac gaaggatttc
     2581 taattccgaa cttgccatag ccgattaagc gtatctttgc ggtcaaatgc aaggatggtc
     2641 tttactgatg aatctttcaa ctccctttaa ggagccggtt atcattttca gtattgtatt
     2701 agcaattatt cttctctctc ccatcctgct taaaactttg cgcattcctt ccattgtggt
     2761 gatgatcatt gcgggagtga taattggtcc acatggcttt caactactgg agcgtgatgg
     2821 tgcgatagta ctttttggaa cagttggttt actctatatc atgttccttg ccggactaga
     2881 aatagacctg catggattta ggaaaaatat atcgcgaagt gtgagttttg gtttgttcac
     2941 cttcatcatt ccatttataa taggttttgc tgtttgtcgc atgattttgc acctagacat
     3001 gattccggca ttaatcaccg cgagcatgtt cagtacgcaa acacttgttg cctatccgat
     3061 cgtaagccga ttaggcatca caagatctgc gcctgttgcg ataacagtcg gcggcactat
     3121 tattaccgat acacttgtgc ttttattgtt ctcggtggtg accaatttcg ccatggataa
     3181 aagtgattca tttttttggt tgcggcttgc catttcagtg atcgcatttg cggctatcgt
     3241 tttatacatc tatccgattt ttgcgaaatg gtttttcagc aaattggaaa atgagggtgg
     3301 atcgcaattt atttttgtgt tgaccttggt gtttatagcg gcatttcttt cgcaattggc
     3361 cggtcttgaa cacatcatag gagcctttct tgccggtatc agcttaacgc gattgatacc
     3421 tcatagttca acattaaaaa atcgcattgt atttgtagga aacaatattt tcatcccctt
     3481 ctttctgatc aatgtaggta tgatcataga tctgcacgtg ttgattgaaa atggcggggc
     3541 attgacagtt gcagcaatat tggttactgt tgcactttcc acaaagtaca tcgctgcatg
     3601 gataacgcaa aaaacattcg gacttacccg caatgaaggc agcctgatat tcggattaag
     3661 cacagcacat gcagcagcta cacttgccat tattttggtt ggatttgacc ttggactatt
     3721 agacgaaaaa atactaaacg gcaccatcat cgtgattttg gtgagttgcc tggtaagctc
     3781 ctttgtaaca gaaaacgccg gaagaaaata tgtgttgctc accgcaaata aggtggatac
     3841 agaccccgag gtaatggagc ggattttagt acctgtcggc aatccggatt ccgcagaaaa
     3901 attattagac cttgcaatga tgatccgcaa tcccggctcg aaggaaccca tttatccttt
     3961 ggctgttgtt gaagatggtc cgcaagctcg tgaacaatta attcgagcaa gacgaaattt
     4021 acaacttgct gcagaacatg tttcatcaag tgaaacgaaa gtacaggtat taacgcgagt
     4081 agacgtgagc attgcaggtg gcatttccgg tgccgtaaaa gaacttggta tcacagaagt
     4141 agtattagga tggcattcaa gagtatcgac gagcgacagg ttatttggaa gtattattca
     4201 gcaggtaata aaaagtacgg atcaaatgtt gtggataggc cgactatcac aaccattaaa
     4261 cacaatagat cgcatcgcca ttttaatgcc accaaatgct gaattcgaat ccggattttt
     4321 cagatggctg gaaggtgtta aacaattgtc gcagcaaaca ggcgcgcgtt gtatgttttg
     4381 gggag
//
LOCUS       DAOQHY010000231         4344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_11972_length_4344_cov_4.703194, whole genome shotgun sequence.
ACCESSION   DAOQHY010000231 DAOQHY010000000
VERSION     DAOQHY010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11972_length_4344_cov_4.703194"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            231..638
                     /locus_tag="PLL28_13600"
     CDS             231..638
                     /locus_tag="PLL28_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70410.1"
                     /translation="MSLTEGTKAAPFKLKTPPLTSDYEMYFDVKDGKDVIVCVVGKTT
                     LLYDRRCLNDLHAMLKNHGDWMELGSADEQKPAKEGTVEAWGRSAQNPVGGWYGLKKG
                     LRGRFGMYIPPLMEKLGLAEVTHDPKGNKMRAK"
     gene            731..1645
                     /locus_tag="PLL28_13605"
     CDS             731..1645
                     /locus_tag="PLL28_13605"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNF70411.1"
                     /translation="MIKTIILLLIAVVAIPLAAFYLDDPLSELQREMLHTSVYMMLAV
                     AAYCFIVGELTHNNSQVDKLWSIIPIVYAGYFAYASDWEPRVTLMSMLVAIWGIRLTY
                     NFSRRGAYSWKFWSGEEDYRWQILRKEPMFNTRLKWMLFDLFFICLYQNTLILLFTLP
                     AVMVTGLNTPLNFWDYLLALLMISAIIFEFIADQQQWNYQNEKLKRINSGAPLEGIYK
                     DGFVRSGLWKYVRHPNYAAEQLIWILFYCFSVAATGEWLNWSAAGFVLLLLLFQGSAD
                     FSEKISSSKYPAYAEYKKTVGRFLPKIK"
     gene            complement(1650..2171)
                     /locus_tag="PLL28_13610"
     CDS             complement(1650..2171)
                     /locus_tag="PLL28_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70412.1"
                     /translation="MMHFIENHTPIFETQVIIDKLAGINDDLHSAGFHFKVDVSDEIG
                     YTSYPNEEIPENTVIVLGSFDFTYYHQCEFAFHQVKAHKLPDYDWRDHWEKPQLILLR
                     GNDRIFALQSLSINDEPGLQVFAFNLGSFGDNCAYLACNAISIFVGTTFYYNRIKDTP
                     LEVGERMAWWVQR"
     gene            complement(2168..2761)
                     /locus_tag="PLL28_13615"
     CDS             complement(2168..2761)
                     /locus_tag="PLL28_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70413.1"
                     /translation="MQKDEIAGSYQLRGIMETAAGLELYPDGKFDFYYVYGAIDRHAY
                     GSWEISNEQIILNSLYTDKTGYDILLSEQRTQPRFTIYFPKADPFFISMQGAFAINGD
                     QAEEQKSDKKGWFLFQTGKAERIMLSNDLFPDRMITIENKDTSHNHIELAPNHDLLLI
                     HFSGVEAMITDDVLHLSSPFLKGMHGDRDFKFYKITQ"
     gene            complement(2769..>4344)
                     /locus_tag="PLL28_13620"
     CDS             complement(2769..>4344)
                     /locus_tag="PLL28_13620"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNF70414.1"
                     /translation="FAGPYALLTTIPAIGTTNYTHVGANGTVTTWYYYIIAVYACAGY
                     TMTTSDTLDNLDPIAPELDYVTVTGGLSEIHWLPSPSAETNSYIIYRDIGGFTPVGTV
                     YGRFTTTFTDPTGTPTTGSETYTIAARDSCNNLGPFNNDAHHTIYLTAQQINCSDQLQ
                     LTWNEYDTWTAGVFEYQVWADRNFTGLSFIDNVPVGSPSYVLSGINDGDHICITVRAL
                     RADGTATSISNEVCLDVNIVVPAAYNVMRNATVTGPTQIAIEWYPDAAADLEKRSVQR
                     STDNVDYVNLITGPIVIPVPAIDSYVDNSIATNSIPFYYRTITTDSCGIETTSGYVKT
                     ILLKGNDNANFTNDISWNDFEITNGTNIEYRIYRDDGAGFNLIATVTPGILTYLDDVS
                     AFVNVVDHFCYQVECLYQLNAPENGVNEQLTSLSNIICLDQGPRIYVPNAIVIGGVNS
                     LFMPVIIFGSSEGYLMQIFNRYGEVIFETTDINAGWDGSYKGSTVPMGTYGYVITFTA
                     TNGQVITKKGNVTVLK"
BASE COUNT         1280 a          849 c          916 g         1299 t
ORIGIN      
        1 agatgccaag gtgtttaata ttagggtgcg gaatgcatat gtttcgcacc ctaatgtttg
       61 tttttgcaat tgtccaaata taattttgat tcagggacgc ttgacaagtc aatttgaatt
      121 ttaaggcgat gttcttcaag gcgcattgca gattcgcata catgggatta tttgttgatt
      181 aaattaaata ttaatcttta gtttagtaat tttatacaaa ttcaaatttg atgtcattga
      241 cagaaggcac aaaagccgcc ccattcaaat taaaaacccc gccgcttaca tcagactatg
      301 aaatgtattt tgatgttaaa gatggtaagg acgtgatcgt atgtgttgtg ggcaaaacca
      361 ctttgctata tgatcgccgt tgcctgaatg acctgcatgc gatgctgaaa aaccatggcg
      421 actggatgga gctgggaagc gccgatgagc agaagcctgc aaaggagggc actgtagaag
      481 cctggggtcg ctccgctcaa aatccggtgg ggggatggta tgggttgaaa aaaggacttc
      541 gcggccgctt cggtatgtat attccgccac tcatggagaa acttgggctc gccgaagtta
      601 cacatgatcc aaaggggaat aagatgcgcg caaagtgagg cagatttcgc tcagcataaa
      661 attgcatttg gtatgttgat actcagttat tagatacaga aaaattcatt ttctttatca
      721 gtcattactt atgataaaga cgattatcct tttgttgatt gccgttgtgg caattccctt
      781 ggcagccttt tatctcgacg atccgctcag cgagttacag cgggaaatgt tgcacacgag
      841 tgtttatatg atgctggctg ttgccgctta ttgttttatt gtaggcgagt tgacacacaa
      901 taatagtcag gtagataaat tgtggagcat cattcccatc gtttatgcag ggtattttgc
      961 atatgcttca gactgggagc ctcgtgtaac actgatgagt atgctggttg ctatttgggg
     1021 tattcgtctg acatacaatt ttagtcgccg aggtgcctac agctggaaat tttggagtgg
     1081 ggaagaagat tatcgctggc agatattgcg caaagaaccc atgttcaata cgcgattaaa
     1141 atggatgctg ttcgatttgt tttttatctg tctttatcaa aacacactta tcctattgtt
     1201 tactctcccg gctgttatgg tgacgggatt aaatacgccg ctaaattttt gggattattt
     1261 gttggcattg ttaatgatat cggcaataat attcgaattc attgctgatc aacaacaatg
     1321 gaattatcaa aatgaaaaac taaaaagaat taattcaggt gccccgcttg aaggaatata
     1381 taaggatgga tttgttcggt cgggattatg gaaatatgtg cgccatccca attatgccgc
     1441 tgagcagttg atctggattt tgttctattg cttttcagta gccgcaacag gcgagtggct
     1501 gaactggagc gcagcaggct ttgtattgct attgctgcta tttcagggaa gtgcagattt
     1561 cagcgaaaaa atatcttcat caaaatatcc tgcatacgcc gaatacaaga aaactgtagg
     1621 caggttttta cccaaaataa aataataaat taacgctgca cccaccacgc catgcgctca
     1681 ccaacttcta acggcgtgtc ttttatccga ttgtaataaa atgtagtgcc aacaaatatg
     1741 ctgatggcgt tgcacgctaa ataagcgcaa ttatctccga aggaaccaag attaaatgca
     1801 aaaacttgta atccgggttc atcattaatg gaaagcgact gcaacgcaaa aattctgtcg
     1861 ttaccgcgga gtaatattaa ttgaggtttt tcccaatgat cgcgccaatc ataatccgga
     1921 agcttgtgcg ctttaacttg atggaatgca aattcacatt gatgataata ggtaaaatca
     1981 aagctaccta agacaatgac tgtattttca ggaatttctt cattcgggta ggaggtgtat
     2041 ccgatttcat ccgaaacgtc aaccttaaaa tgaaatcctg cactatgtaa atcatcgttg
     2101 atccctgcaa gtttgtctat gataacttgt gtttcaaaga ttggagtatg attctcaata
     2161 aaatgcatca ttgcgtgatt ttgtaaaatt taaaatcacg atcaccatgc atgcctttta
     2221 gaaatggtga actcaagtgc aatacatcat ctgtaatcat tgcctccaca ccgctaaaat
     2281 gaataagcag caggtcgtga tttggggcaa gctcaatgtg attatgtgat gtatccttgt
     2341 tttcaatagt aatcattctg tcgggaaaga gatcattact caacatgatt ctttcagctt
     2401 tgcctgtttg aaataaaaac caaccctttt tgtcggattt ttgttcttca gcctgatcac
     2461 catttattgc aaatgcgccc tgcatgctta tgaaaaatgg atctgctttc ggaaaatata
     2521 tagtaaaccg tggctgtgta cgttgttctg aaagtaatat gtcgtaaccg gttttatctg
     2581 tatacagaga gttcaaaatt atttgctcat tgctgatctc ccaactgcca tatgcatgtc
     2641 tgtcgatggc gccatacaca taataaaagt caaacttacc atcgggatac aattccaatc
     2701 ccgcagccgt ttccataatc cctctcaatt gatatgaacc tgcaatttcg tccttttgca
     2761 tgcgtatgtt atttcaatac ggtaacattc ccttttttgg tgatcacctg tccattggta
     2821 gccgtaaatg tgatcacata accgtaagtt cccataggta cagtgctgcc cttgtaagaa
     2881 ccgtcccaac cggcattgat atcggtagtt tcaaaaatta cttcaccata gcgattaaat
     2941 atttgcatga gatatccctc cgaagaaccg aaaatgatta caggcatgaa taaggaattc
     3001 acaccaccta taacaattgc attcggcaca taaatacggg gaccctgatc aaggcagatt
     3061 atattggata aggaagtgag ttgctcattt acgccgttct caggtgcgtt taattgatag
     3121 agacattcaa cctgatagca gaaatgatca accacattta caaatgcaga tacatcatcg
     3181 agataggtta aaattccggg tgttactgtt gcaatgagat taaaccctgc accatcatct
     3241 ctgtaaatgc gatattctat attagtgcca ttggtaatct caaaatcatt ccagctgata
     3301 tcattggtga aattagcgtt atcatttccc ttcagcaaaa tcgtcttcac atatcctgat
     3361 gtagtttcta ttccacaact gtcagtagta atcgtgcgat aataaaatgg aatagaattg
     3421 gttgctatgc tattgtctac ataactgtca atggccggta cgggaattac aatcggtcct
     3481 gtgatcagat tgacataatc aacattatca gtgctgcgct gaactgatct tttttccaga
     3541 tcggcagcgg catcgggata ccattcaata gcaatttgtg tgggcccggt aactgtagca
     3601 ttgcgcatta cattgtaggc agcggggaca acaatattta catcaagaca aacttcatta
     3661 gaaatggaag tggcagttcc atctgcacga agtgctctca ccgttatgca gatatgatcg
     3721 ccatcattga ttccggaaag tacatacgat ggagaaccca ccggaacatt gtctataaaa
     3781 ctcaaaccgg taaaattcct atcagcccaa acctgatatt caaatacacc ggctgtccag
     3841 gtatcatatt cattccacgt taattgcaac tgatcggaac aattgatttg ttgcgctgta
     3901 agataaatag tgtgatgtgc atcattgtta aaaggaccta aattattaca tgaatcgcgt
     3961 gcagctatgg tatacgtttc agatcctgtt gtaggtgttc cggtaggatc tgtgaatgtt
     4021 gtagtaaatc gaccgtatac agtacctaca ggtgtaaacc ctccgatatc acgataaatg
     4081 atatatgaat tagtttctgc cgaaggtgat ggcaaccagt gtatttcaga taagcctcct
     4141 gtaacggtaa cgtaatccaa ttcaggagca atcgggtcga gattatctaa cgtgtcgctg
     4201 gtggtcatgg tataaccggc acaagcatat acagcaatga tataataata ccaggtggta
     4261 acagttccgt tagcaccaac atgcgtataa ttcgttgtgc caatggcggg aatagttgtg
     4321 agaagcgcat agggccccgc aaaa
//
LOCUS       DAOQHY010000232         4272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_12303_length_4272_cov_5.794166, whole genome shotgun sequence.
ACCESSION   DAOQHY010000232 DAOQHY010000000
VERSION     DAOQHY010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12303_length_4272_cov_5.794166"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..693
                     /locus_tag="PLL28_13625"
     CDS             166..693
                     /locus_tag="PLL28_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70415.1"
                     /translation="MRPLILVLLICAVHSLQAQQVPRFAKYPIGDSGCEGYFPGDPEF
                     EMTWSDDSSKVYTGEVALDSFNYFAICVKLSEPQSASQREQLDLLESYMDFLQEALEV
                     TGTAGYGEGHTLEYYPSVSGVIDYWEDAGGNSYTVKGWTNTQYLVVLGIYGQGEYPYF
                     NAGQMYLEGIRFPEE"
     gene            761..4156
                     /locus_tag="PLL28_13630"
     CDS             761..4156
                     /locus_tag="PLL28_13630"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNF70416.1"
                     /translation="MKQLFTLLLAACCTYANGQLLINEIAPNNSGYYDDEDNTFPDWI
                     ELVNTGATTLNVGGYAITDDMDTWNQWLLGNYNIPAGGRLRIFASAKNRDCFTCGGVV
                     SEMHTNFKLSYGETLALYDDAGNLLDSLTIPTLQAGHTMARIPDGGTWCYSGTPTPNA
                     TNTGTCYSGYAAAPTFNTQPGFYTGSVDVTASGAEVRYTTDGDWPGFASTLYTTPIHV
                     TANTVVKLTGIEAGKLPSRVVTGSFFIDEPTLLPVVSISSNSCDMFDEGASCIAAYDG
                     ADGWEPDNPQISAAVEYFTADKVQQFEKRIKFETAGNSSIFVYPQRGMQFTCDEDFGE
                     TSEFQFNLFAASKPDLDSLNGFRVRANNDWGNSAARMKDVINNRIALPTNIVVPAYQN
                     VATFINGEYWGHYSAREELDAYFLRNNLGVNPDKVDLIRSGAGEDVWDIAEFGTMTAY
                     NELSTFFNTHNMADPDDYALALGLIDKENWIDHFALQFFVNNDEMAYNLRCFRSYDPD
                     MKWKFILWDTGAGSECETCNSIQSLINFPYLSDEINMMNDLMDNEDFKIDFINRYADL
                     MNYYFTWDRIESLIDVNADEIEAEIPAQNARWGTGTLATWNNGVSELKAFFDNRVYYQ
                     RNEIENYLDMNDQVNVTLEVSPAGAGYIKISTIIPQELPWTGVYFDGAPVTVTVMANP
                     GYTFSNWTDNPFIASETDPSFTVNITENTTFTANFTGAAIANPIYISEINYNSDSSLQ
                     SGDWIEIHNTAGVAVDVSNYTFSNNYFYNKFLIPSGTIIPANGFLVLVEDTALFTSMH
                     PDVSSFVGPLHFPIRNEGDSVTLRNAVGEVLISFAFNDKRPWPITADGYGRTLEFPYY
                     FTDPSLPENWLAGCIGGSPGLPYTPCIENPVIEEINYKSAITEDAGDWFEFYNWAASP
                     FDLSGWKVMDKNKNVFTIPNGTQIPGGGGYYVVYQDETKFFAQFPTVTNATGPTIFGF
                     DGEGDVISIFDQDGQLRYSIGYDDEAPYPLSPDGGGTALQMINTALNGNDPANWTESC
                     PEGSPGTEFVMPCANSIEQQEPELSLQVSPNPGSTLFNISIPGMTGEASILIADVTGN
                     IIYNGELIHTNEFIIDLTKYPAGIYHVRAVNGDYTYTAQIVKL"
BASE COUNT         1241 a          888 c          923 g         1220 t
ORIGIN      
        1 aaacatcaat atttgaacaa aataaatgag tctaaatata cttttcaacg ccttaaatgt
       61 ccaatttttt tatatttacc ctgtcacaat ttacataaaa cccttacata aattgcccat
      121 cacttaccct caaatattca ttcaaattaa aatatctttg aaaacatgcg tccacttatc
      181 cttgttttac tgatttgcgc tgttcacagt ctacaagcac agcaagttcc aagatttgcc
      241 aaatatccta ttggcgactc gggttgtgaa gggtattttc ccggagatcc ggaatttgag
      301 atgacatggt cggatgacag ttcaaaggtt tataccgggg aggtggcctt agattccttt
      361 aattattttg ccatttgcgt caagttgtct gagccgcaaa gtgcctctca acgggagcaa
      421 ctcgacctgt tggaatcgta tatggacttt cttcaggaag cactggaagt gacggggact
      481 gcgggatatg gagaggggca tacattggaa tactacccgt cggtttccgg cgtaattgat
      541 tattgggagg atgccggagg aaattcctat accgtgaaag ggtggacaaa cacccaatat
      601 ctcgtagtcc tgggcattta tggacagggc gaatacccct attttaacgc aggccaaatg
      661 tatcttgaag gtattcgatt cccggaggaa taatcttacc ttttccgggt attgttaagc
      721 gaatgttacc tgcctggtga attttgggta tattagtgct atgaagcagc tttttacact
      781 cctcctcgct gcctgctgca cttatgctaa cggacagctt ctaatcaatg aaattgcccc
      841 gaacaactca gggtattacg acgacgaaga caatactttc cctgactgga tcgaactggt
      901 gaatacgggt gctaccactt tgaatgttgg cggctacgca attacagacg atatggatac
      961 ctggaaccaa tggttattag ggaattataa tattcctgcc ggcggtcgct tgcgcatatt
     1021 tgcatctgca aaaaacagag attgttttac atgtggcggt gtggtttctg aaatgcacac
     1081 caattttaaa ttgagttacg gtgaaacact tgctttgtat gacgatgccg gaaatctgct
     1141 cgatagtctg accattccaa ccctgcaagc tggtcatact atggcgcgca ttccggatgg
     1201 cggcacttgg tgctattcag gaacacctac accgaatgcc acgaacacag gaacttgcta
     1261 ttccggatat gctgcggcac cgacctttaa tacacaaccc ggattttata ccggcagtgt
     1321 cgatgttact gccagtggtg ccgaagtgcg atacacaact gatggcgatt ggcccggatt
     1381 tgcatcaacc ttatatacaa cacctattca tgttactgca aatactgtag tgaaacttac
     1441 aggaattgag gcaggtaaac ttcccagcag agttgttacc ggatcattct ttatagatga
     1501 accaacttta ttacctgttg taagtattag ctccaatagt tgcgatatgt ttgacgaagg
     1561 tgctagctgc atcgccgcat acgatggcgc agatggatgg gaacccgaca atccgcaaat
     1621 aagcgcagca gtggaatatt ttactgcaga caaagtgcag cagtttgaaa aacgaatcaa
     1681 atttgaaact gcgggaaatt catcgatttt cgtttatccg caacgaggta tgcaatttac
     1741 atgcgatgaa gattttggtg aaacaagtga atttcaattc aatttatttg ctgcttcaaa
     1801 acccgatctc gattcgttga acggtttcag agtgcgcgca aacaatgact ggggaaactc
     1861 tgcagcacgc atgaaggatg tgatcaataa ccgcatcgcc ttacctacta atattgtagt
     1921 acctgcttat caaaacgtag ccacatttat caatggagaa tattggggac attattctgc
     1981 ccgcgaagaa cttgatgcat atttcctgag aaataatctt ggtgtaaatc ccgataaagt
     2041 tgatctcatt cgatcaggcg ccggtgaaga tgtttgggat attgcggaat tcggcacgat
     2101 gaccgcctac aatgaattgt cgactttctt taacacgcat aatatggcag atcccgatga
     2161 ttatgcattg gcattgggat tgatagataa agaaaactgg atcgatcatt ttgcattgca
     2221 gttttttgtg aacaatgacg aaatggcata caatttacgc tgcttcagat cgtatgatcc
     2281 tgacatgaaa tggaaattta ttttatggga tacaggggca ggaagtgaat gtgaaacatg
     2341 caacagcatt caatcactca ttaactttcc atatctgagt gatgagatta acatgatgaa
     2401 tgacttgatg gataatgagg atttcaaaat tgactttatc aatcgttatg ccgatctgat
     2461 gaactattat ttcacctggg atcgcatcga atcgctaatc gatgtaaatg ccgatgaaat
     2521 tgaagctgaa attccggcac aaaacgcacg ctggggaacg ggaactctcg caacgtggaa
     2581 taacggagta tcagaattga aagcattttt cgacaaccga gtatattacc agcgcaatga
     2641 gatagagaat tacctcgaca tgaatgatca ggtaaatgtt acattggaag tatctcctgc
     2701 cggtgccgga tatattaaaa tttctaccat tatcccacaa gaactccctt ggacaggcgt
     2761 gtattttgat ggagcacctg ttaccgttac cgtgatggca aatccgggtt atactttcag
     2821 caactggaca gacaatccat ttattgccag cgaaacagat ccatctttca ctgtaaatat
     2881 cactgagaac actacgttca ctgccaactt tacaggtgcc gccattgcga atccgattta
     2941 tatcagtgaa attaattata acagcgactc aagcttacaa tcaggtgatt ggatagaaat
     3001 tcacaatact gcaggtgtag ctgttgatgt aagtaactat actttcagca ataattattt
     3061 ttataataaa tttcttatcc cttccggcac tatcattcct gcaaacggat ttttggttct
     3121 ggtagaagat accgcattat tcacttcgat gcatcctgat gtttcttcgt ttgttggacc
     3181 attgcatttc ccaattcgca acgaaggcga tagtgtaacc ttgcgaaacg ccgttggtga
     3241 agtattaatt tcatttgcat tcaatgacaa gcgcccttgg ccgatcaccg cagatggtta
     3301 tggtagaaca ttagaattcc catattattt taccgatccg tcattgcctg aaaactggct
     3361 tgcaggatgt ataggaggct cgcccggatt gccatataca ccttgcattg aaaatccagt
     3421 gatagaagaa atcaattaca aatctgctat tactgaagat gcaggcgact ggtttgagtt
     3481 ttacaattgg gcagcgtcgc cgttcgattt gagtggttgg aaagtgatgg ataaaaacaa
     3541 aaacgtattt accattccaa atggaacgca aattcccgga ggtggcggat attatgttgt
     3601 ttatcaggat gaaacaaaat tctttgctca attcccaact gttacaaatg caacgggacc
     3661 tacaattttt ggatttgatg gtgagggtga tgtcatttcc atttttgatc aagatggtca
     3721 attgcgttac agcattggct acgacgatga ggctccttat ccgctcagtc ctgatggtgg
     3781 cggtactgct ttgcaaatga taaacactgc attgaatggc aatgaccctg caaactggac
     3841 agagagttgt cctgaaggaa gtccgggtac cgagtttgta atgccttgcg ccaacagtat
     3901 cgaacaacaa gaacctgagt tgagtttaca agtatcacca aatccgggat cgacattatt
     3961 taatatcagc attcccggaa tgaccggtga agcaagtata ctcattgcgg atgtaacagg
     4021 aaacattatt tataatggtg aattgataca taccaatgaa ttcattatcg atctcacaaa
     4081 atatcctgca ggtatatatc atgtccgtgc tgtaaatggc gattacacct acacagcgca
     4141 aatcgtgaag ttgtagatca cctcactccc ccgcctcacc cccggcccct cctccatctc
     4201 ctatcgtcga tagagagggg agctgattgc gcttcggaat taaaatattg caaagcaata
     4261 taattgcatt cc
//
LOCUS       DAOQHY010000233         4269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_12319_length_4269_cov_6.634314, whole genome shotgun sequence.
ACCESSION   DAOQHY010000233 DAOQHY010000000
VERSION     DAOQHY010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12319_length_4269_cov_6.634314"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..1937
                     /locus_tag="PLL28_13635"
     CDS             207..1937
                     /locus_tag="PLL28_13635"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNF70417.1"
                     /translation="MHQVSLFIRRFSISALLMLLVFSAQGQTDWTFKKEVVDSVLELQ
                     YFSTQMPGYAMCIIKDGKIVYTGVRGKSSIKLNQKMSSTTQFNIGSVTKQFTAAAIYL
                     LEEEGKLSLSDNVKKYIPEFPDYPTAITIDQLIHHTSGIRDHIEVATLLSKYKQDLNE
                     LDGMLSWIQKYPELNAAPGTSFAYSNTNYMLLAVIIERLSGMKYEDFLDTQIFKPLGM
                     QATYVERGKHKTLRDGTTHYDINYKQTHAKPADAIQNAPGATGVITTLEDLVKWDNNF
                     YHNILGKKNQSLINKMEQSAVLVDGSDTHYGAGLLVTNYRFKPVIEHSGGWGQYLTQY
                     RRFPDEGVTVIVCTNAYLMSPFVMCDNVCNTLFNYKNLHLFTQNKIPEIVSKQICGPY
                     AAENNLIRYIYTKNNNLYIARIAEGDTTAYPMRYVGPMDNGYAFMDTTGKSISIVMEG
                     NKPHHLSWDGGTYFGAMRNYQYIDTTLEINFYKYHGKYYCAALDKKIRIQYFSKEDEL
                     TIHPFPFIKYNLISKGGDYYQVEGQPYIVHFGNGHLLIGNDWVYNIRADKKIKRHIDL
                     WPFEKKSSEK"
     gene            complement(1957..>4269)
                     /locus_tag="PLL28_13640"
     CDS             complement(1957..>4269)
                     /locus_tag="PLL28_13640"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70418.1"
                     /translation="NGTDGGDGGGLVLIEANQIIGNSHSIISNGVTVPSMAWGDGAGG
                     GGAGGTIALVCPSVSALTISANGGKGGDTGADQCTGPGGGGSGGVLKHYGATIWPGVI
                     VNLNGGTFGTNITATSDCFGLNNGAAAGSTGSTISNFIAAESITPYVADFANAGSDVT
                     VCNGGSIMLNASGGVSYSWSPATYLDNPAISNPTCTPLATTNYVVTVTNANGCTDIDN
                     ITVTLAPPAVANAGDDISICSGESTTLNGSGGVSYSWSPSIFLDDAFAANPIASPLID
                     IIYTLTVTDINGCVGTDEVAITVNSSNFLTTSGDVDACGGGDVMLHAVGADSYLWTPS
                     TYLDDPTSANPICSPLTDITYTITAISTEGCVDVDSLSVNIIPAAFLNAGDDVSTCTG
                     ADVILNATGGVVFHWSPSTYLDDADIASPTCTPLSDIIYIVTSETADGCTDTDTISVS
                     VLPSDFADVDATSPVCTGTSTTLHASGGTTYNWSPSTYLDDATVANPVCTPLTDITYF
                     VSVTNADGCVDVDTVTVNTIPSPVITAGPDTTVCFGGQIKLFVTEGVAYNWTPATFLD
                     YTTIESPTASPTATISYIVYVTDASGCIGSDTVDIIVNSIPDITASADITICRGDTAT
                     LTAVGGVEYTWTPDPLIPCIDCSSIDVAPTSTTTYIVQGIDANGCSATDEVTVTVDIC
                     DAINNILAEEIQIFPNPTTGNFQISCPKSANFQSLELFNLIGEKVNCEVKSDQSNITV
                     NVAESHSGQLLLLIRFDEGTFVEMITILGE"
BASE COUNT         1339 a          855 c          856 g         1219 t
ORIGIN      
        1 ttatcctgaa aactctatag ataagatccg cgagttgaac gatatgggta aagatgaaac
       61 cttgaaaaca ggcgtgaaga tcaaattggt gaaataatcg ccgcctccct gatttttggt
      121 tgaccaataa accactcgga accatttcgc gagcagggaa ttatttgtca tataatttgt
      181 cgagttgctg ccgtaatttt gagcatatgc atcaggtatc cctattcata cgtcgcttca
      241 gtatctctgc attattgatg ctattggttt tttctgcaca aggacaaacc gattggacct
      301 ttaaaaaaga agttgttgat tctgttcttg aattgcaata tttcagcacg caaatgccgg
      361 gttatgcgat gtgtattatt aaggatggta aaattgtgta taccggggtg cggggaaaga
      421 gctcgataaa attaaatcag aaaatgtcat ctaccacaca attcaatatt ggttcggtga
      481 ctaaacaatt taccgctgct gcaatttact tgttggaaga ggaggggaaa ctcagtcttt
      541 ccgataatgt aaaaaaatat atacctgaat ttcctgatta cccgacagcg attactattg
      601 atcaattaat tcatcatact tcaggcataa gagatcatat tgaggttgca acgctgttga
      661 gtaaatacaa acaagatttg aatgaacttg atggcatgtt atcatggatt caaaaatatc
      721 ccgaattaaa tgctgccccg ggaactagtt ttgcttattc caataccaac tatatgttgt
      781 tggctgtaat cattgaacgt ttgtcgggaa tgaagtatga agattttcta gacacacaaa
      841 tattcaaacc tctgggcatg caggcaactt atgttgaaag aggcaaacat aagacacttc
      901 gcgatggcac aacacattac gatattaatt acaaacaaac ccatgccaaa cctgcggatg
      961 ctattcaaaa tgccccggga gcaacgggtg taattacaac attagaagat ctcgtaaaat
     1021 gggacaataa tttttatcat aatatattgg gtaaaaagaa tcagtcgctg attaataaaa
     1081 tggagcaatc agctgtactt gttgatgggt ctgatacaca ttacggcgcg ggattgcttg
     1141 taaccaatta tcggtttaaa ccggttattg aacatagcgg aggatggggc caatatttaa
     1201 cacaatatcg caggtttcct gatgaaggtg ttaccgttat tgtttgcact aacgcctatt
     1261 taatgtcacc atttgttatg tgtgataatg tttgtaacac cttattcaat tataaaaatc
     1321 tccacctgtt cacacaaaat aagattcctg aaattgtaag taaacagatt tgtggaccat
     1381 atgctgcgga gaataatctt attcgataca tatacacgaa gaacaacaat ttatatatcg
     1441 cccgaattgc ggaaggagac actacagctt atccaatgcg ctatgtaggc ccgatggata
     1501 atggatatgc ttttatggat acaacaggca aaagtatttc cattgttatg gaaggtaata
     1561 agccacatca tctttcttgg gatggaggta cttattttgg ggcaatgcgc aattatcaat
     1621 acatagatac cacactggaa atcaattttt ataagtatca tggaaaatac tactgcgcgg
     1681 ctctggataa gaaaattcgt atacagtatt tttcaaaaga agatgaattg accatccacc
     1741 catttccatt tatcaaatat aatttgattt caaaaggggg tgattattat caagtagaag
     1801 gacaacccta tattgttcac ttcggtaacg ggcatttatt aatcggcaac gactgggtat
     1861 ataatatacg tgccgacaaa aaaattaaaa gacatattga cctctggcct ttcgaaaaga
     1921 aatcttcgga aaaataggtt gaattaaata cgcgccttat tcgcccaata ttgttatcat
     1981 ttctacaaaa gttccctcat caaatctgat caacagaagt aattgccctg aatgggattc
     2041 agcaacatta acagtgatat tactttgatc tgattttact tcacaattaa ctttttctcc
     2101 gatcagatta aataattcca acgattggaa attagcagat ttcggacatg agatttggaa
     2161 atttcctgtt gtgggatttg gaaatatttg tatttcttct gcaagtatat tattgatggc
     2221 atcgcaaatg tcaactgtta cagttacctc atcggtagcg ctacagccat ttgcatcaat
     2281 accctgtact atataagtag ttgttgatgt gggtgctaca tcgatactgc tacaatcaat
     2341 acatggaatg agtggatcgg gtgtccaagt atattcaact ccaccaacgg cggtcagcgt
     2401 agcggtatct ccccgacaaa tagttatgtc tgcagaggca gtaatatccg gaattgaatt
     2461 tacaataatg tcaacagtat ccgatccaat acatccactt gcatcggtta catagacaat
     2521 atagctgatt gttgcagttg gacttgcagt tggactttca attgtagtat aatctaaaaa
     2581 tgttgccggt gtccagttat aggcaacacc ttctgttaca aataatttta tttgtccgcc
     2641 aaaacatact gtcgtatccg gaccggcagt aataaccgga gatggaattg tatttacggt
     2701 aacagtatct acatcaacac aaccgtcggc atttgtaaca cttacaaaat aggtaatatc
     2761 ggtgagtggt gtacaaaccg gatttgcaac agttgcatca tcaagatacg ttgaagggct
     2821 ccaattataa gtggtccctc ccgaagcgtg taaagttgtt gaggttccgg tacacacagg
     2881 cgatgttgca tctacatcag caaaatcgga aggaagtacg gaaaccgata tcgtatctgt
     2941 atcagtacat ccatctgctg tttcagatgt aacgatataa ataatatcac tgagcggggt
     3001 acatgttggc gatgcaatat cggcatcatc aagataggtt gaaggactcc agtgaaatac
     3061 aacaccacct gtggcattta atatcacatc ggcacctgtg catgttgaaa catcatcacc
     3121 ggcatttaaa aatgcagcag gaataatatt taccgaaaga gaatctacat caacacaacc
     3181 ttcagtactt attgctgtga tagtatatgt aatatcagtt agcggactgc aaattggatt
     3241 tgcagatgtt gggtcatcta aataagtaga aggagtccat aaataactat cggcacctac
     3301 agcatgtaac attacatcgc caccaccaca agcatcaaca tcgccgctgg tagttaaaaa
     3361 gttagagctg ttcactgtta tggcaacttc atcagtacca acacatccat taatatccgt
     3421 aacggttagt gtataaataa tatctattaa tggcgaagca ataggattag cggcaaatgc
     3481 atcatctaaa aatatcgatg gcgaccagct atatgaaaca ccgcctgaac cattcaatgt
     3541 agtagattca ccactacaaa tgctgatatc atcgcctgca tttgcaactg caggaggagc
     3601 aagtgtaaca gtaatgttat cgatatccgt acaaccattt gcatttgtaa ctgtcaccac
     3661 ataattagta gtagccaatg gtgtgcatgt tggatttgaa attgcgggat tgtctaaata
     3721 agttgcagga ctccatgaat acgatacacc acccgaagca tttagcatta tactaccacc
     3781 gttgcaaaca gtaacgtcgc tgccggcatt ggcaaaatct gcaacataag gagttatact
     3841 ttctgctgcg atgaaattac ttattgtgct gcctgttgaa ccggctgctg cgccattatt
     3901 cagaccgaag caatcgctgg tagcggtaat atttgttccg aaggtgccgc cattgagatt
     3961 gacgataacg cccggccaaa ttgttgcgcc ataatgtttg agtacaccac cggagccacc
     4021 gccaccggga cctgtacatt ggtcggcgcc ggtatcacca cctttgccgc cgtttgctga
     4081 aatggttaag gcagaaaccg aagggcaaac taaggcaata gtacccccgg caccgccacc
     4141 gcctgcgcca tcgccccatg ccatgcttgg gacagttacc ccatttgaaa taatactatg
     4201 gctattgccg attatttgat ttgcctctat gagcaccagt ccgccgccat caccgccatc
     4261 agttccatt
//
LOCUS       DAOQHY010000234         4197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_12665_length_4197_cov_5.463786, whole genome shotgun sequence.
ACCESSION   DAOQHY010000234 DAOQHY010000000
VERSION     DAOQHY010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12665_length_4197_cov_5.463786"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1683
                     /locus_tag="PLL28_13645"
     CDS             <1..1683
                     /locus_tag="PLL28_13645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNF70419.1"
                     /translation="EVKQVANFNHTLYAQVADTIYQYDGTSWMPWLYEENWHTNSISV
                     DEQAMVVTQVFGINSPADSSRIITLLADGTITDLTNDVDLLFPSEATLGDDGELWIAD
                     LYRGLLAYDGAQFKSYLPNGPGTSDVTDMKSAGNVLYVAPGEINASWNYQYNHAGFFA
                     YDYGFWNTLNQYGFGILDSVYDFITVDVDKKNNVVYMGSFGGGLVAYDRSTGVMQLYK
                     QGYLENAVGDPGSYRVGGLAVDSSSNLWISNFGSIDPIAVRKADGTWAHFNPGLTADV
                     FNQLGQIAIDEFNTKWIQLPRGNGILVFNEQGTIDDQSDDLIKVLGAGAGNGNLHTNY
                     INCIAVDKQSEIWVGTSEGITIFYNPSEVYTGTTAGDATQPLVNLGGYYEQLLRNDIV
                     NTIAVDGANRKWVGTNSGAFLISADGTEQLLYFNSDNSPLISNTVLNIEIDGTTGDVY
                     FGTDKGIISYRYTATEGVGEITSVKAFPNPVREDYTGSIAISGLTADAEVRITDMAGR
                     TIYQTIALGGQAVWDGNGYDGKRAATGVYMVYASNEDGSETAVSKILVISSK"
     gene            1771..2487
                     /gene="recO"
                     /locus_tag="PLL28_13650"
     CDS             1771..2487
                     /gene="recO"
                     /locus_tag="PLL28_13650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNF70420.1"
                     /translation="MLHKTRGIVLRTTRYGDTSVIAKVYTEAFGLQSYIVNGVHTSKN
                     TGKGSLYQHGNLLEMVVYHKEQGALMRISECRFSHIYEQLPFNVIKGGLLLFYIELLN
                     RIIREEEQNEALFDFLFESFIDLDQTDKKIGAHHIWFLLGLSKYLGFYPNICTGHIFD
                     LEEGAFTQLMPRHTNYISENGAMYLRNCIDPELKNYDQMQIPASDRRSLVHWLINYYK
                     LHLSDFGEVRSLDVLEGLFR"
     gene            complement(2495..3223)
                     /locus_tag="PLL28_13655"
     CDS             complement(2495..3223)
                     /locus_tag="PLL28_13655"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNF70421.1"
                     /translation="MSLLPWQNATVVDVVAHNAHTKSYFLAAEGVEVFAFKPGQFVTF
                     DLPIADRPSQRMRSYSIASAPGNNNVFELVVTHKPNGAGSGYFFDHCSIGHQLKFRGP
                     QGTFVLPDSFNLPIALICTGTGVAPFRSQVKYLLENNITIPEIHLVFGTRTLSDALYK
                     EEFESLAAAHPHFHYHLTLSRPDHNWKGKHGYVHELYTDICKNGTVDMDFFLCGWRMM
                     VNEARTRLQDLGYPKERVHLEVYD"
     gene            3358..>4197
                     /locus_tag="PLL28_13660"
     CDS             3358..>4197
                     /locus_tag="PLL28_13660"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNF70422.1"
                     /translation="MEWLALPEKYREHDDRIKQLHEQIGEKPQNLHLKGIAGSLPSFV
                     ASAIYKKQQRHHLFILNDKEEAGYFLNDIETLFPGKDVLFIPDSFKKQNSFDEISTNN
                     ILQRTEAINRIAHSHTRGEIIITYPEALVEKFVIPEALEKQIIYIKKGEKLDDQFIID
                     VLIENGFDRNDFVYEPGQFSVRGGIIDIFSFGNDLPYRIELFDDEVESIRVFDPASQI
                     SQKMIAQVTIVPNIQTQFREEQKASLLNTLPKDTLVWCKDARYTFEVINQVYEKAHDW
                     YAVV"
BASE COUNT         1273 a          781 c          936 g         1207 t
ORIGIN      
        1 gaagtgaaac aagttgcaaa ttttaatcat acgctttatg ctcaggttgc cgataccatt
       61 tatcaatacg acggcacttc ttggatgccc tggctgtatg aagaaaattg gcataccaat
      121 tccatttctg ttgatgagca ggcgatggtt gttacgcagg ttttcggaat taattctccc
      181 gcagactcta gtcgtattat cacactttta gccgacggca ccataacgga tttaaccaat
      241 gatgttgatc tgcttttccc gagtgaagca acacttggag atgatggtga gctatggatt
      301 gccgatcttt atagggggct attagcatat gatggcgcgc agtttaaatc gtatttgcca
      361 aatggtcccg gcaccagcga tgttaccgat atgaaaagtg caggcaatgt actttatgtt
      421 gctcccgggg aaataaatgc ctcttggaat tatcaatata atcacgcagg ttttttcgcc
      481 tatgattacg gattctggaa tacgcttaat caatatggtt tcggtattct ggatagtgtt
      541 tatgatttta tcaccgttga tgtagataag aaaaataatg tcgtttatat gggatcattc
      601 ggtggcggtc ttgttgccta tgatcgctca acgggagtta tgcaattgta taaacagggc
      661 tatctcgaaa atgcagtggg cgatccgggg tcttatcgcg ttggagggtt ggcagtggat
      721 tcgagcagca atttatggat atcgaatttt ggatcgattg acccaatagc cgtgcgtaaa
      781 gctgatggta cctgggcaca ttttaatccg ggattaaccg cggatgtatt taatcagttg
      841 ggacaaatag ccattgatga atttaatacg aaatggatac aattacctcg tggtaatggt
      901 attctcgtat ttaatgaaca aggcaccatc gacgatcaat cggatgattt aataaaagtg
      961 ctgggtgccg gagccggaaa tggaaatctc cataccaatt atatcaactg tattgccgtt
     1021 gataaacaaa gtgagatctg ggtgggcacg agtgaaggca ttacaatttt ttataatcct
     1081 tctgaagtgt ataccggaac aacagcaggc gatgcaacac aaccattggt taatcttggg
     1141 ggatattacg agcaattatt gcgcaatgat attgtaaata cgattgcagt tgacggagca
     1201 aacaggaaat gggtgggcac caacagcggc gcatttttga tcagcgctga cggcactgaa
     1261 caattattat attttaattc cgataactca ccgttaatat cgaatacggt attaaatatt
     1321 gaaatagacg gaactacggg tgatgtttat ttcggtaccg ataagggtat tatttcatat
     1381 cgttatacgg caactgaagg agtgggagaa ataacaagtg taaaggcatt tccaaatccg
     1441 gtaagagagg attatacagg gagcatcgca attagcggac tcacagcaga tgctgaagta
     1501 cgcataacag atatggcggg ccgaacaatt tatcaaacca ttgcgcttgg tggtcaggca
     1561 gtgtgggatg gcaacggata tgatggtaaa cgcgcagcta caggtgttta catggtgtat
     1621 gcttctaatg aggatggcag cgaaacagcg gtttccaaaa tacttgtaat ttcttcaaaa
     1681 taatttcggc ttatttgttt accggatttg ttgaaataaa tcgcaacatt aatttcggct
     1741 attagctagc tttacaaaaa gatttttccc ttgctccata aaaccagagg cattgttttg
     1801 cgcaccacac gatatggtga taccagtgtg atagcaaagg tatataccga agcttttgga
     1861 ctgcagagct atattgtaaa cggtgttcat acgtcaaaaa acacaggaaa gggttcctta
     1921 tatcaacacg gaaatctgct cgaaatggta gtgtatcaca aagagcaagg ggcattgatg
     1981 cgcattagtg aatgtcgatt ctcgcatatt tacgagcaac taccctttaa tgtaattaaa
     2041 ggtggcttgt tattgtttta tatagaattg ttaaatcgca ttattcgaga agaggaacaa
     2101 aacgaagcac tttttgattt tttatttgag agcttcatcg accttgatca aacggataaa
     2161 aaaattggtg cacatcatat ctggttttta ttgggactga gtaaatacct tggattttac
     2221 cctaacattt gtaccggcca tatatttgat ttggaggaag gcgcctttac ccaattaatg
     2281 ccgcgacaca ccaattatat ttctgaaaac ggagctatgt atcttcgaaa ttgtatagac
     2341 ccggaactaa aaaattacga ccagatgcaa attcctgctt cggatagaag aagcctggtg
     2401 cattggttaa tcaattatta taaattgcat ttaagcgatt tcggagaagt gcgttccctt
     2461 gatgtgctgg agggattgtt tcgatgacat tacatcaatc atatacttcc agatgcacgc
     2521 gttcttttgg gtagcctaaa tcttgcaggc gggttcgtgc ttcattcacc atcatgcgcc
     2581 agccacacag gaaaaaatcc atatctaccg taccattttt acaaatatca gtatataact
     2641 catgcacata accgtgcttg cctttccagt tgtgatcagg cctgcttaag gtaaggtgat
     2701 agtgaaaatg cggatgtgct gctgcaagcg attcaaactc ttctttataa agtgcgtcgc
     2761 ttaaagtgcg ggttccgaaa actaaatgta tctcgggaat agtgatgtta ttctctaaaa
     2821 gatatttcac ctgtgagcga aatggcgcaa caccggtgcc tgtgcaaatc aaagctatag
     2881 gcagattaaa tgaatcgggc aaaacaaatg taccttgcgg tccgcggaat tttaattgat
     2941 gtccgatgct gcaatgatca aagaaataac cggatcctgc gccattgggc ttatgtgtaa
     3001 caactaattc aaatacatta ttatttccgg gagctgaggc tatggaatag ctgcgcatgc
     3061 gttgacttgg tctgtcggct ataggtaggt cgaaggttac aaattgtccc ggtttaaatg
     3121 caaacacctc aacaccctca gctgcgagga aatagctctt tgtgtgtgca ttgtgggcaa
     3181 caacatcaac cactgttgca ttttgccatg gtaataaaga cataatgcaa aggtaaggtc
     3241 ttgaagtagt aaaaagttca ttaaataatg aaaactgact ttaattcgga ccaaattaat
     3301 tgcacatgga aactaattcc agcaagtcaa acggcatttc ttatctttgc acctttcatg
     3361 gaatggttag cgcttcccga aaaatacagg gagcatgatg ataggattaa gcagttacat
     3421 gaacagatag gagaaaagcc tcaaaacctc catctgaagg gcattgcagg ctctttaccg
     3481 tcgtttgtag cctccgcaat ttataaaaaa cagcaacgtc accacctgtt tattctcaac
     3541 gataaggaag aagccgggta ttttttgaat gatattgaaa cattgtttcc tggaaaggac
     3601 gtattgttta ttcctgattc gtttaaaaag caaaatagtt ttgatgagat cagcacaaac
     3661 aatatccttc aacgcacaga ggcaattaac agaatagcac attcgcatac tcggggtgaa
     3721 atcattatca cctatcctga ggctttggtc gaaaaatttg ttattcccga ggcgcttgaa
     3781 aaacagatca tctacataaa aaaaggcgaa aaacttgatg accagtttat cattgatgtg
     3841 ctcattgaaa acggattcga tcgcaacgat tttgtatatg aacccggcca gttttcggta
     3901 cgaggaggaa tcatagatat tttttctttc ggcaacgacc tgccctatcg catagaatta
     3961 tttgacgatg aggttgaaag tatacgtgtt ttcgaccccg cttcgcagat atcgcaaaaa
     4021 atgattgcgc aggtaaccat agtgccgaat atccaaacac agttccgcga agaacaaaag
     4081 gcttcgctat taaatacact accgaaggat acactcgttt ggtgtaaaga tgcaagatat
     4141 acctttgaag taataaatca agtttacgag aaagcgcacg attggtatgc ggttgtc
//
LOCUS       DAOQHY010000235         4177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_12766_length_4177_cov_5.629549, whole genome shotgun sequence.
ACCESSION   DAOQHY010000235 DAOQHY010000000
VERSION     DAOQHY010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12766_length_4177_cov_5.629549"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..2113)
                     /locus_tag="PLL28_13665"
     CDS             complement(26..2113)
                     /locus_tag="PLL28_13665"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HNF70423.1"
                     /translation="MENTKKDILWRLYLTFAGIVLLCLAIIAQIVNVSFVHGETYRAM
                     ADSVRTKQVSIPPARGSIYSDKHEVLATSFPYFNIYMDPCAASEVDFRRDVDSLSIAL
                     ARLFKDKPASAYKQKITTARKQGKKYLEIHKRVTLPQKQLMQTFPLFRIGQNGGGFIV
                     EPYEKREYPYGSLAVRTIGYVRNGRGIGLEGAYDTLLSGKPGMAVMRKVAGGSWIRLS
                     EQNEIDPVDGVDIITTLDINMQDITETALRNALVKNNAKWGTAVVMEVKTGKIKAISN
                     LSRESEGVYHEDYNYAILEKVEPGSTWKLFSLMCLFEDGLSINDHVDLNFGQYPFADR
                     MMKDSEPHGRRDVTVKTAFALSSNVGISRLAWDMYQKDPESYVKHLQESGIVNVTGVE
                     LPGEPKPYFKQDPSDKNHWYKTTVPWMSVGYELLITPLQLATFYNAVANDGVMMKPYL
                     VEETQQHGKTLQKFEPVVLNEKLCSSKTIDQLHQCLLAVVDSGTAKHLKNDYYKFAGK
                     TGTAQLLENGVYGHNYLSSFAGYFPYDNPKYTIVIIINSPSNGPFYGGAVAAPAFREI
                     ADKVYSHFVNMREPVNAPSAANTGVQAFSKGYAYDYRQIFKTLKVNEDFNDNEEWIAV
                     SANGNNSEHTELKTYQYTMPDVRGMGLRDAMYLLDTYGLNVAVSGAGKVTIQSIPPGT
                     AIQKDNYVMLTLE"
     gene            complement(2100..2468)
                     /locus_tag="PLL28_13670"
     CDS             complement(2100..2468)
                     /locus_tag="PLL28_13670"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HNF70424.1"
                     /translation="MAQQEKNNKGKEQKRSLASMLKSFDEVAFFKWENILANIPYFLF
                     LVVIGIFYIWNNHRGVAMDRDIRDLNTHLLEKQYFYNATSDSLTQQSRQSTVARMVDT
                     LQMQELSHPPYTIKTNDGKH"
     gene            complement(2483..3406)
                     /gene="rsmH"
                     /locus_tag="PLL28_13675"
     CDS             complement(2483..3406)
                     /gene="rsmH"
                     /locus_tag="PLL28_13675"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNF70425.1"
                     /translation="MSEAYHIPVLLHPSIQALITDPDGIYVDATFGGGGHSKAIVEQL
                     SPKGKLFCFDQDPDAKKNFIDAFSATNATFIPQNFRHMQKFLRVNGVSKVHGILADLG
                     VSSHQFDTGDRGFSIRFDGPLDMRMAQQGTQSAYALINESSAEQLQYIFGHYGEVRNA
                     RQLANAIVQTRNLFAATGGIRTTTQLRDVALTVLKGEKNPYLAQVFQALRIAVNDEMR
                     ALEEMLESAASLLMPQGRLVVISYHSLEDRLVKNYMRAGNAAGEAEEDIMGRKNVPFK
                     VITRKPVVADDEELSINPRARSAKMRVAEKI"
     gene            complement(3403..3858)
                     /gene="mraZ"
                     /locus_tag="PLL28_13680"
     CDS             complement(3403..3858)
                     /gene="mraZ"
                     /locus_tag="PLL28_13680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNF70426.1"
                     /translation="MAFLIGEYECTLDVKQRIRVPAGLLGQLTGDDAGKFVITRGIDP
                     CLYLYPMSEFHIIMDKVNQIPEYSEEDREYKHMFFSGTSILQIDSADRILLPKLHLAH
                     AGIVKEMVMTCEKNKVVVWSMERFREKFLNRSSLDYQQLAEKVLGKYGL"
BASE COUNT         1114 a          958 c          840 g         1265 t
ORIGIN      
        1 tgagatgata tgtcttttaa taatttcatt ccagggttag cattacataa ttatcttttt
       61 gaatggctgt tccgggaggg atgctttgaa tggtaacttt tcctgctccg gaaacagcaa
      121 catttaaacc gtaggtatct aacagataca tggcatctcg caatcccatt ccacgcacat
      181 caggcatcgt atattgatat gtttttaatt ctgtatgttc cgaattattt ccatttgcag
      241 aaacggctat ccattcttca ttatcattaa aatcttcatt cactttgagt gttttaaaga
      301 tctgacgata gtcgtaggca tatccctttg aaaaggcctg tacaccggta tttgcagcac
      361 ttggcgcatt taccggctct cgcatattta caaaatgcga atacacctta tccgcgattt
      421 ccctgaaagc cggagctgct acagcaccac cataaaatgg accgtttgaa ggcgagttaa
      481 ttattattac aatggtatat tttggattat catacggaaa gtaacctgca aaacttgaca
      541 agtaattatg accgtaaacg ccattttcca gcaactgtgc agtacctgtt ttacctgcga
      601 atttgtaata atcgtttttc aaatgtttag cagtaccgct atccactaca gccaacaaac
      661 actgatgcaa ctgatcgatt gttttgcttg aacaaagttt ttcatttaaa acaacaggtt
      721 cgaatttctg caatgtttta ccatgctgtt gtgtttcttc caccaaataa ggtttcatca
      781 tcacaccgtc attggctaca gcattataaa aagttgccag ttgcaatggt gtgatcaaca
      841 attcatatcc gacactcatc catggcacag tagttttata ccaatggttt ttatctgatg
      901 gatcttgttt gaaatatggc ttaggttctc ccggtaattc cacaccggtt acattcacta
      961 tgccggattc ttgtaaatgt tttacataag actccggatc tttttgatac atatcccacg
     1021 ccagtcttga tatacctaca tttgatgata aagcaaaagc agtcttcacc gtaacatcac
     1081 gtctgccatg tggctcacta tccttcatca tacgatcagc aaaaggatat tgcccaaaat
     1141 ttaaatcgac atgatcattg atagataatc catcttcaaa caaacacatc aatgaaaata
     1201 atttccatgt agaacccggc tccacttttt cgaggatggc ataattataa tcctcatggt
     1261 aaacaccttc cgattcgcga ctgagatttg aaatggcttt aatttttccg gttttcactt
     1321 ccattaccac tgctgtcccc cactttgcat tattttttac taatgcattg cgcagtgcag
     1381 tttcggtgat gtcctgcata ttgatatcga gtgtggtgat gatatctaca ccatctaccg
     1441 gatcgatttc attttgctcg cttaatctaa tccatgaacc acctgcaact ttgcgcatca
     1501 cagccatgcc gggtttacca ctcaataaag tgtcataggc accttcgaga ccaatacctc
     1561 ttccattgcg cacatagcca atggtacgca ctgccagact gccatatgga tactcgcgtt
     1621 tttcatacgg ctctacaata aatcctcctc cattttgccc gatgcgaaac aatggaaatg
     1681 tttgcatgag ttgtttttgc gggagggtaa cgcgcttatg aatctcgaga tattttttgc
     1741 cctgtttgcg tgctgtagta attttctgtt tatatgcgga ggcaggttta tctttaaata
     1801 aacgagcgag agcaatactc agtgaatcta catcgcgacg aaaatctact tcagatgccg
     1861 cacatggatc catataaata ttgaaatatg gaaatgatgt ggcgagcacc tcatgtttat
     1921 cagaataaat agaaccacga gcaggcggaa tgctcacttg tttcgtgcgc acactgtcgg
     1981 ccattgcacg ataggtttca ccatgcacaa aagacacgtt gacgatctgt gcaataattg
     2041 ccagacacag caatactata cctgcgaagg tgaggtataa acgccataat atgtctttct
     2101 tagtgttttc catcgttcgt tttaatggta tatggcggat gcgataattc ttgcatctgc
     2161 agtgtgtcta ccatgcgcgc cactgtgctt tggcgactct gttgtgtgag agagtcgctt
     2221 gtggcgttat agaaatattg tttttcgagg agatgcgtgt tcagatcacg gatatcgcga
     2281 tccatagcaa caccgcggtg attattccat atataaaaaa taccaatcac tacgaggaac
     2341 aagaaataag ggatattcgc gagaatattt tcccacttaa aaaaagcaac ctcatcaaat
     2401 gatttcagca ttgatgcaag gcttcttttt tgttcctttc ctttattatt tttttcttgt
     2461 tgcgccatag ttggtttgcg cgttatattt tttctgccac acgcattttt gcactgcgag
     2521 cgcgtggatt tattgataat tcttcgtcat cggctaccac aggttttctg gtgatcacct
     2581 taaatggtac attttttctg cccatgatat cttcttcagc ttcacctgct gcatttcctg
     2641 cccgcatata atttttcacg agcctgtcct caagactgtg ataactgatt accaccagtc
     2701 tgccctgcgg catcagcaat gaagctgcac tttcgagcat ttcttccagt gcgcgcatct
     2761 catcgttcac ggcgatgcga agcgcttgga atacttgagc caggtatgga tttttttcgc
     2821 ctttgagtac tgtcagcgcc acatctcgca attgtgtggt ggtgcgaata ccgccggttg
     2881 ctgcgaagag atttcttgtt tgcacaatgg cattggcaag ttgccgtgca ttgcgtactt
     2941 cgccgtagtg gccaaaaatg tattgcaatt gttcagcgct gctttcattg atcagggcgt
     3001 atgccgattg tgttccctgc tgtgccatcc gcatatcgag cggaccatcg aagcgtattg
     3061 aaaaaccgcg gtcgccggta tcgaactgat gcgagctgac acccagatct gccagaatac
     3121 catgcacctt tgaaacacca ttcacccgca gaaacttctg catatggcgg aagttctgcg
     3181 gaatgaaagt tgcgttagta gcgctaaacg catcgatgaa attttttttt gcatcgggat
     3241 cctggtcaaa gcaaaataat ttcccctttg gagaaagctg ctccacaatc gccttgctat
     3301 gcccgccgcc tccaaaagtg gcatctacat agataccatc ggggtcggtg atcaatgcct
     3361 gtatgctcgg atgtaaaaga acaggtatgt gatacgcctc gctcataaac catatttgcc
     3421 gagtactttt tcagcaagct gctggtaatc gagtgaagag cggttgagga acttctcacg
     3481 gaatcgctcc atagaccaaa ccaccacttt gttcttctcg caagtcatca ccatttcctt
     3541 tacaataccg gcatgcgcca aatgcaactt cggcaacaaa atgcggtctg cagaatcgat
     3601 ctgaagaatg gaagtaccgc tgaaaaacat gtgcttatac tccctatcct cctccgaata
     3661 ctccggaatc tggttcacct tgtccattat tatatgaaat tcactcatcg ggtatagata
     3721 aaggcatggg tcaataccac gcgtgatcac gaactttccg gcgtcatcgc ctgtgagctg
     3781 ccctaacagc cccgcaggaa cgcggatacg ttgtttcacg tcaagagtac actcatattc
     3841 accgatcagg aatgccatat tgctggtctt ggagctaatt tgaacgtaaa cttagacaga
     3901 ttattacact tttgtacact tttgtacact tcggtgcttt gaaaattttt gcacacggaa
     3961 tgcacatggt ttatacacaa atgtgagaat caatgaaata cgctgcagga aagggtttga
     4021 gcttggtttg tagggagctt ttcacaccct gtgcataaag tagcgatgag gcccgctttg
     4081 gaggaccact tacacagcaa ggctacttca ttccaatacc aaagctccgt cgaatcgaag
     4141 ggaatggacg ggaatttgaa gcttattaca ggtattc
//
LOCUS       DAOQHY010000236         4168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_12806_length_4168_cov_5.662777, whole genome shotgun sequence.
ACCESSION   DAOQHY010000236 DAOQHY010000000
VERSION     DAOQHY010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12806_length_4168_cov_5.662777"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            342..1034
                     /locus_tag="PLL28_13685"
     CDS             342..1034
                     /locus_tag="PLL28_13685"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNF70427.1"
                     /translation="MKALLKKIQFKLAGLLLLFRIQPDAHAQASAHNNFDDEINYYKN
                     LDDTTTLPEHPVLFVGSSSVKNWIGFDSTYGQFGGLNRGFGGSTLLDQIHYANDIILN
                     HDPKQIVIYCGENDFAASSSVTPDLVFDRFLQLYKIIRSKYPGVPVVCISMKPSPSKL
                     EMMDKIYAYNALLQDYIAVDSSITYADIFTPMLNPDGSPDRSYFFSDMLHMNKKGYQV
                     WNSVVLPLIIKQ"
     gene            complement(1100..2974)
                     /locus_tag="PLL28_13690"
     CDS             complement(1100..2974)
                     /locus_tag="PLL28_13690"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNF70428.1"
                     /translation="MHFSLRNTLLAGSLAFAMPLFAQPKLDLQLVAGPAFDSPVDIAN
                     CGDSRLFIVEKSGYIRILNTDGTSSLFLDIDAKVKSSGSEQGLLGLAFDPNYAANGYF
                     YVNYTNNTATGNTTIARYTRNAVNPNIADVASEVILLNIVQPFSNHNGGNLEFGPDGY
                     LYIGTGDGGSSGDPGNRAQDITDQLLGKMLRIDVSTAPYSIPPSNPFVGITGDDEIWQ
                     YGLRNPWRFSFDRATGDLWIGDVGQNAWEEVDFQPAASTGGENWGWRCYEGNATYNTT
                     GCAGIGSYDFPIYVYNHSFATGGYAITGGYVYRGSDYPGMYGYYIFCDYVSGNWWTTV
                     SNGAGGWTSAKNDLTFGDISTFGESSDGEIYCANLNTGLIYKVIDLCSNFIGTTAVTD
                     ANYGFNNGAVNLTVSGGTSPYSYSWSNGATTEDISALAAGAYNVTITDANACTASASA
                     TVNSVCGALPSITTTSVTSTTASFDWIDEGVASYKVQYRPSTGGAWTHVNTPVSNITI
                     TGLTPATSYKLKVSHTCPGGAKYSKNKNFMTPARLDGSEVVLPQIAPNPSSGVINISN
                     MTPQTEVMVFDLSGKQINAGIDVQAGTLDLNNVAEGLYMIRFMENGMLISSEKVLIAR
                     "
     gene            3201..>4168
                     /locus_tag="PLL28_13695"
     CDS             3201..>4168
                     /locus_tag="PLL28_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70429.1"
                     /translation="MRSVLLSLILVSLLAACEMPENIQDGQTAYKLKKYKLAAELLQK
                     DYAKAELPDEKAKIAYQIGESYAYNNRYEQAADWYFKSSDLGYGSEATLQYALMLKAQ
                     EKYPEAMQYFNQYLIDEPFRRPEITIELNACQNAIDWMNRQQNEYERDTYVTRLNTLN
                     SGDADFDPVLFGNDKIIFTSSRTSSTGDNTDSWTGNKFYDLYVATRQGINNFSAPEAF
                     TGPFNSPYNDGSITFSRDGQEVFFTRCGSDDRKIDDYCGLYMSNYLGDNNWSDPIALP
                     FFEDTMNIGTPCLSPDGQTLFFAAVNPDGYGGADLYMSKRNAEGWDA"
BASE COUNT         1237 a          894 c          834 g         1203 t
ORIGIN      
        1 tggtttcgcg caaagacagc aaggaggcta aggcgcaaaa gtctagatac tagattcttg
       61 attctagata ctgaaagaag gttagcaagt tagcaggtgg ggggtgcgcg caaaggcgca
      121 aagttgcaaa ggcgcaaaac ttatcactca ccgactgacc cactcaccca ctcacgagtc
      181 acgattcacg tatcctgccc gcaactgacc tcggaattga aaatatatat ttccgccttg
      241 tgaaaattta catttaggga cgggctcgaa cagctaatat tataaatttg caaaattcat
      301 tgcaacattc taaaggtaaa ctccgttata ttattccaaa catgaaggca ttgcttaaga
      361 aaatccaatt caaacttgcg gggttattac tacttttcag aatacaaccc gatgcacatg
      421 cgcaagcttc tgcgcacaat aatttcgatg acgagattaa ttattataaa aacctcgacg
      481 acactaccac acttcctgaa catcctgttt tatttgttgg ctcctcgagt gtaaaaaact
      541 ggattggctt cgattcaacg tatggtcaat ttggtggatt gaaccgcggg tttggcggat
      601 ctaccttgct ggatcaaatt cattacgcca atgacatcat tctcaaccat gatccaaagc
      661 agattgttat ttattgcggg gagaatgatt ttgcagcgtc atcaagtgtt acgcctgacc
      721 tcgtgtttga caggttcctg caactataca agatcatacg ttccaaatat cccggcgttc
      781 cggttgtttg tatttcgatg aaaccatcgc cttcaaaatt ggaaatgatg gataaaatat
      841 atgcttacaa tgccttatta caagactaca ttgccgttga cagttctatc acttatgccg
      901 atattttcac acctatgctg aatccggatg gaagtcccga cagaagttat tttttcagcg
      961 acatgctgca tatgaataaa aaaggttatc aagtttggaa tagcgttgtc ctgccattaa
     1021 taataaaaca ataatttact gcaataaaaa aaggctgcgg aaaattactt cgcagccttt
     1081 tttttatgtt cgataacaat taacgtgcta tcaatacttt ttcactcgaa ataagcatgc
     1141 cgttttccat gaaacgaatc atgtataatc cttcagcaac attattaagg tctaatgttc
     1201 ccgcctgaac atcaattccg gcattaattt gttttcccga caaatcaaaa accatcactt
     1261 ctgtttgtgg ggtcatatta gaaatattaa tgacaccgct gcttgggtta ggtgcaattt
     1321 gtggtaatac cacttcactg ccatccaacc ttgcaggtgt catgaaattc ttgttcttgc
     1381 tgtatttcgc gccacccgga caagtatgag aaactttcag tttatagctt gtagcgggtg
     1441 ttaaaccggt aatggtaata ttactcaccg gtgtatttac atgagtccac gcaccacccg
     1501 ttgaagggcg atattgcact ttatacgatg ccaccccttc gtcaatccaa tcaaagcttg
     1561 cagtagtgct tgttactgat gttgtggtga ttgatggcaa tgctccgcaa acactgttta
     1621 ccgttgcaga tgcagatgcg gtgcaagcat tcgcgtcagt gatagttacg ttgtaagctc
     1681 ctgcagccag cgcactgatg tcttcagttg ttgcaccatt actccaacta tatgaataag
     1741 gcgatgtgcc tcctgataca gttaaattaa ctgcaccatt attaaatcca taattcgcat
     1801 cggtaacagc agttgtgccg atgaaatttg agcagagatc aattacttta taaatgagac
     1861 cggtatttaa attagcacaa taaatttcac catcactact ttctccgaat gtagaaatat
     1921 cgccgaatgt taaatcattt ttcgccgaag tccatccacc tgcaccgtta gaaactgtgg
     1981 tccaccaatt tcctgaaaca taatcgcaga aaatataata tccatacatg ccgggataat
     2041 cgcttccacg ataaacgtaa ccgcctgtta tagcataacc gcctgtagca aatgagtggt
     2101 tatatacata aataggaaag tcatatgatc cgattcctgc gcatccggtg gtattatagg
     2161 tagcattacc ttcataacaa cgccatcccc agttttctcc tccggtacta gcggcaggtt
     2221 gaaaatcaac ttcctcccag gcattttgac caacgtcgcc aatccaaaga tctcctgttg
     2281 cacgatcgaa agaaaaacgc catggattgc gcagtccgta ttgccaaatt tcgtcatcgc
     2341 cggtaattcc aacgaaagga tttgatggcg gtatggaata tggagcggta cttacatcaa
     2401 tgcgcaacat tttacccaat aactgatctg taatatcctg cgcgcgattt ccggggtcgc
     2461 cactgcttcc gccatcgcct gttccgatat ataaatatcc gtcaggaccg aattccagat
     2521 ttccaccatt gtggtttgag aagggttgaa caatgtttaa taaaattacc tcgcttgcca
     2581 catcagcaat attcgggttg acagcatttc tggtgtatcg tgcaatggtc gtatttcccg
     2641 tggctgtatt atttgtgtaa ttcacataga aatatccgtt tgccgcataa ttcgggtcaa
     2701 aggcaagtcc aagcaatcct tgttcactgc ctgaactttt tactttcgca tcgatatcca
     2761 gaaataatga gctggtgcca tcagtattca atattctgat gtagcccgac ttttcgacaa
     2821 taaacaatct gctatcgccg caatttgcaa tatcgaccgg tgaatcaaac gcgggaccgg
     2881 caaccaactg caggtcgagt ttaggttggg caaataatgg catagcgaat gccagactgc
     2941 ccgccaatag ggtgtttcgt aatgaaaaat gcatacaaat gtctttttta gtgaatgaat
     3001 gattgcctgt gtgacaaacg aggaggcgtt tgcagttcta aaataataaa atatgtgtaa
     3061 acagatagtt ttttacacat tttttcttcg ataaaacctc catttcgtat gatttttgcg
     3121 gtaaaaccca tgaatcatga caaaacaatg ggacatacaa caaaatcttt cccttaacgt
     3181 tttatacttt tgataactat atgagaagcg ttctcctcag tttgatcctt gtaagcctgc
     3241 tggcagcttg cgaaatgcct gaaaatattc aggatggaca aactgcctat aaattgaaga
     3301 aatacaagct tgctgcggaa ctcctgcaga aagattatgc taaagcagag ctacccgatg
     3361 aaaaggctaa aatcgcgtat cagataggtg aaagttacgc ctacaataac cgttatgagc
     3421 aagccgccga ctggtatttc aaatcgagtg atctcggtta tggcagtgaa gcaacgttgc
     3481 aatatgcatt gatgcttaag gctcaggaaa aatatcctga agccatgcag tattttaatc
     3541 aatacctgat agatgaacca tttcgcagac ctgaaataac gatagaatta aatgcatgcc
     3601 agaatgccat cgattggatg aacaggcaac aaaatgaata tgaacgcgat acctatgtaa
     3661 cgagattaaa caccttgaat agtggtgatg ccgatttcga tcctgtattg tttggcaatg
     3721 ataaaataat atttacttca tcgcgcactt cttcaacggg tgataacact gactcgtgga
     3781 caggaaataa attctacgat ttatatgtag ccactcgtca ggggatcaat aatttttcag
     3841 cacctgaagc atttacaggt ccgtttaact ctccatataa tgatggctct ataaccttca
     3901 gtcgcgatgg tcaggaagta ttttttactc gctgcggtag cgatgatcgc aaaatagatg
     3961 attattgcgg attgtacatg agcaattatt tgggcgataa caattggagt gatcctattg
     4021 ctcttccttt ctttgaagat accatgaaca tcggaacacc atgtttatca cctgacggac
     4081 agacactttt ttttgcggcc gtaaatcccg atggctacgg aggtgctgat ctttatatga
     4141 gcaaacgcaa tgccgaggga tgggatgc
//
LOCUS       DAOQHY010000237         4167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_12812_length_4167_cov_4.616245, whole genome shotgun sequence.
ACCESSION   DAOQHY010000237 DAOQHY010000000
VERSION     DAOQHY010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12812_length_4167_cov_4.616245"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2974
                     /locus_tag="PLL28_13700"
     CDS             <1..2974
                     /locus_tag="PLL28_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70430.1"
                     /translation="YYNGTMDMDPGPGTSNITSAGSDDIFVGKYDTNGSLIWAYSIGG
                     TGTEQNYGFDVGPDNNPVLYGYFQNTVDFNPGGGTFNLTAAVGGSNFILKLNNNGTFN
                     YAVSMGSAYGNELKIDAANNIYCTGLFWSTVDFDPGVATYNLTAAGFTNDSYIQKLSP
                     TGGFLWAGKFSSSLADQGTCIAIDNINNTVSVGGFFEGTIDADPGIGTANIASGGYVD
                     AYVINLNASTGNYNWGKSFSGLGWQALHSMDVSSTGSVIVAGSFDQTADFNPGAGTFN
                     MTSAGSTDIFKVALNSDGSFSSAEQIGSNGQDQPICLAIDQEGAVLLTGFFENIVDFD
                     PGANVFNLNAAFTGWDGFIAKYCTVYTINNYVNICSGDSYFAAGANQTTSGDYYDYYT
                     PVEGCDSIVITHLTVNNPTVNLGPDANICAGTTFTLDAENTGADYLWNTGATTQTINI
                     TATGTYSVTVTDDAGCIAADAITVTVQAAPVLELGPTIHSCADEFVLLDAGNAGADYL
                     WNTGATTQTISVNTSGNYSVTVTNDFGCTTSDLVHVTVHALPVVDLLADEGICEGETL
                     LLDAGNPGSTFAWNTGATTQTITVSDADTYSVIVTNAWGCDASDAITITINPTPVIDL
                     GADISLCDGESIILDATADDATYLWNTGATTSTITVTETGDYNVEVSNIFGCSAVDNI
                     HVEVHPLPIVDLGEDAAFCEDEFMVLDAENPGSTYLWNTGETTSTIIVTDDGTYSVDV
                     ISAFGCVSSDTISITIHPLPVADLGADVTSCSNVPVLLDATTPFCLYAWSTGETTPNI
                     VVDESGEYWVTITNSFGCQITDSITVSMLALPEVDLELEPTICVQDPAFTLSGGTPSG
                     GTYFGDGIIDGIFNPAAAGTGTHNIYYTYTDANGCSDTAMVSVTVNICNGIADNNATA
                     ISVFPNPAMNDITINLPSNLTSAEYVFMQADGRAALSGTAQSNLQVIDISTLAAGYYT
                     LIIADEGNIISAPVFVTK"
     gene            complement(3045..3788)
                     /locus_tag="PLL28_13705"
     CDS             complement(3045..3788)
                     /locus_tag="PLL28_13705"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF009668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNF70431.1"
                     /translation="MDSNLLNPTPIPAMPRIGDNAPVFEAETTQGHINFPADYAGKWV
                     ILFSHPADFTPVCTTEFMTFATMEQEFQNLNCQLVGLSIDSIYSHIAWLRTIKEKIAF
                     RDMKNVEVTFPLIDDIRMEVAMKYGMIQPNESNTKAVRAVFFIDPVGKIRAIIYYPLS
                     TGRNFEELKRVLIALQTADAFGVATPADWKPGDDVIVPPAGSCGVAKERMQGKDEQVY
                     CVDWFFCMKPLEKEKIDTLFVRKNEIVLN"
     gene            3885..>4167
                     /locus_tag="PLL28_13710"
     CDS             3885..>4167
                     /locus_tag="PLL28_13710"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HNF70432.1"
                     /translation="MASVTFTTLIKKFGSMGEKTGWTYVEVPFEYADKLNPGVKTSYR
                     VKGKLDDLSVKQLALLPMGGGDFIFPLNTDMRKKLGKRAGNSLTLKINID"
BASE COUNT         1198 a          855 c          884 g         1230 t
ORIGIN      
        1 ttattacaat ggcaccatgg acatggatcc cggccccgga acatcgaata ttacctctgc
       61 cggcagtgac gatatttttg tagggaaata tgatacaaat ggaagtttaa tttgggctta
      121 ttccattggc ggaaccggca cggaacagaa ctatggattc gatgttggtc ctgataacaa
      181 ccctgtttta tatggttatt ttcagaatac cgtagatttt aatcccggtg gcggaacgtt
      241 taatcttact gctgctgtag gtggttcaaa ttttatttta aaattgaata ataacggcac
      301 gtttaattat gcagtttcta tgggctctgc ttatggaaat gaattaaaaa tagatgccgc
      361 caataatatt tattgcaccg gtttattctg gagcactgtg gattttgacc caggcgttgc
      421 aacatacaat ttaactgcag ccggattcac aaatgattct tatattcaaa agttatcacc
      481 caccggagga tttttatggg cagggaaatt cagcagtagc ctcgctgatc agggaacttg
      541 cattgctatt gataatatca ataatacagt atccgtaggt ggtttttttg aaggcaccat
      601 cgatgctgat ccgggcatag gtacagcaaa catagcttcc ggaggatatg tagatgccta
      661 tgtaataaat ctcaatgcat cgacaggaaa ttataattgg gggaagtcat ttagcggact
      721 tggatggcaa gcgctgcatt cgatggacgt ttcatcaaca ggcagtgtta ttgttgccgg
      781 ttcgtttgat caaactgccg attttaatcc gggagcaggt acctttaata tgacttctgc
      841 cgggtcaaca gacattttca aggttgcatt aaacagtgat ggatcctttt cttcagccga
      901 acaaattggt tccaacgggc aagatcaacc aatatgttta gctattgatc aggagggcgc
      961 tgtattgcta accggatttt ttgaaaatat tgttgatttc gatcccggtg caaatgtatt
     1021 taacttgaat gcggcattta ctggttggga tggatttatt gcaaaatatt gcacggtgta
     1081 taccatcaat aattatgtaa acatttgttc cggcgacagc tattttgccg caggtgcaaa
     1141 tcaaacaaca agtggcgatt attacgatta ttatacacct gttgaagggt gtgatagtat
     1201 cgtaatcaca catctcaccg taaataatcc aacagtaaat ctcggtccgg atgcaaacat
     1261 ttgcgcaggc acaacattta cactggatgc agaaaataca ggtgccgact atttatggaa
     1321 caccggtgcc actactcaaa caataaatat cactgcaaca ggcacttatt ctgtaactgt
     1381 tactgatgat gccggctgca ttgctgctga cgccataacg gtaacagtac aagctgctcc
     1441 tgttttagaa ctcggcccta ctattcattc ctgcgccgat gaatttgtat tactggatgc
     1501 cggaaatgca ggtgccgatt atttgtggaa tacaggggca accactcaaa ccatttctgt
     1561 taataccagc ggcaattatt ccgttaccgt taccaatgat tttgggtgta ccacttcaga
     1621 tcttgttcac gtaactgtgc atgctttacc tgtagtagat ctgcttgctg atgaaggaat
     1681 ctgtgaaggc gaaacactgc tgttggatgc cggaaatccg ggttctacat tcgcctggaa
     1741 tacaggagca acgacacaaa ctattactgt atcagatgcc gatacatata gcgtaatagt
     1801 taccaacgcc tggggatgtg atgcttctga tgccataacc attacaataa atccaacacc
     1861 ggttattgat cttggtgccg atatttcatt atgcgatggt gaatccatta tactcgatgc
     1921 cactgcggat gatgccactt atttatggaa taccggagca acaacatcaa caatcaccgt
     1981 aactgaaacc ggcgactaca atgttgaggt tagtaatatt tttggatgct ctgctgtcga
     2041 caatatccat gtagaagttc atcctcttcc aatagttgat cttggagagg atgcagcatt
     2101 ctgtgaagat gaatttatgg tgctggatgc agaaaatccg ggttccacat atctttggaa
     2161 taccggtgaa acaacatcaa caattattgt tactgacgat ggtacttata gtgtggatgt
     2221 aatttctgca tttggatgtg tatcttctga tacaatttcc atcaccattc atccacttcc
     2281 ggttgctgat cttggtgccg atgtaacttc ttgttcaaac gtaccggtac tgttagatgc
     2341 cactacacct ttctgtttgt atgcatggag tacaggagaa actacaccaa acatagtagt
     2401 tgatgaaagc ggtgaatatt gggtaaccat tacaaattcc ttcggttgcc agatcacaga
     2461 tagcattact gtttcaatgt tggccttacc ggaagttgat ctggaattag aaccaacaat
     2521 ttgtgttcag gatccggcat tcacattaag cggaggaaca ccttcaggtg gtacttattt
     2581 tggcgacggc attattgacg gcattttcaa tcccgccgct gcaggcacag gaacacacaa
     2641 tatttattac acctacacag atgcgaatgg atgcagtgat acagcaatgg tttctgttac
     2701 cgtgaatatt tgcaacggca tagcagataa taatgcaact gcaatttcag tatttccaaa
     2761 tccggcgatg aatgatatca ccatcaattt gccaagtaat ctgacatcgg cggaatatgt
     2821 tttcatgcag gccgatggaa gagccgcttt aagcggaaca gcgcaaagca atcttcaggt
     2881 tattgatatt tctacgcttg ccgcgggcta ctatacgctc attattgcgg atgaagggaa
     2941 tattatatcc gctcccgttt tcgtcacaaa ataaaaaaca gcagcatggc atactgaacc
     3001 atagccatgc tgcttaacat acaccttaat tcaaaaagat gagatcaatt aagcacgatc
     3061 tcatttttcc gtacgaatag tgtatctatt ttctcttttt ccaatggttt catgcagaaa
     3121 aaccaatcaa cgcaatatac ttgttcatct tttccctgca tgcgctcttt tgcaactccg
     3181 catgaacctg caggaggaac aattacatca tcaccgggct tccaatcggc aggagtggca
     3241 actccgaagg catcggcagt ttgtaatgcg atgagcacgc gttttaattc ttcaaaattc
     3301 cttcctgtcg acaatggata ataaatgatt gcgcgaattt tcccaaccgg atcaataaaa
     3361 aatactgccc gaactgcttt tgtattgctt tcgttcggtt gtatcatgcc atatttcatg
     3421 gcaacttcca tgcgtatatc atcgataagc gggaaggtaa cttccacatt tttcatatcg
     3481 cggaatgcaa ttttctcctt tattgttcgc aaccaggcta tatgactata gatgctgtcg
     3541 atagacaaac caaccaattg acaattcagg ttttgaaatt cctgttccat ggtagcgaaa
     3601 gtcataaact cagtagtgca aacaggcgta aaatcagcag gatggctgaa tagaattacc
     3661 catttgcctg cataatcggc cgggaaattg atatgaccct gcgtggtttc agcttcaaaa
     3721 acgggggcat tatcccctat cctgggcatg gcagggatag gtgttggatt taataagtta
     3781 ctatccatat taggttattt ttttatttat gatgcaaagt tcgtatgatc aatgccgcta
     3841 aagagtgata tttgtaacac aacattaaag tatttaatat cagtatggca tcagtgactt
     3901 tcaccacctt aatcaagaaa ttcggctcta tgggagaaaa aaccggctgg acttatgttg
     3961 aagtgccatt tgagtatgcc gataaattga atcccggtgt caaaacctcg taccgggtga
     4021 aaggaaaatt agatgatctg tctgtaaaac aattagcatt attgcccatg ggtggtggcg
     4081 acttcatatt tccgctcaat accgacatgc gtaaaaaact tggtaagcgc gcgggaaatt
     4141 ccctcacctt gaagatcaat atcgaca
//
LOCUS       DAOQHY010000238         4156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_12861_length_4156_cov_6.472080, whole genome shotgun sequence.
ACCESSION   DAOQHY010000238 DAOQHY010000000
VERSION     DAOQHY010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12861_length_4156_cov_6.472080"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="PLL28_13715"
     CDS             <1..705
                     /locus_tag="PLL28_13715"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNF70433.1"
                     /translation="GSDSMPKLMADLQPKIDSIFDKTNYEVRITGTSVVALSGYQYLI
                     DSLFESLLIAFIGISLILLYQFRSLRMLAISFLPNLIPLFITGAIMGFMHIAIKPSTV
                     LVFSIAYGMCVDFTCYFIAKYKHDLIRNSFNIPKTVMSSLQEAGVSMIYTSLILFCGF
                     FIFVFSKFGGTVNMGLLTSITLLVALFNNLLLLPALMLLFEKGLNKSNRKFIEKSSHE
                     KASRPFTDLIDEHQKN"
     gene            complement(768..1019)
                     /locus_tag="PLL28_13720"
     CDS             complement(768..1019)
                     /locus_tag="PLL28_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNF70434.1"
                     /translation="MSKQTTIIDVRTPAEFIGGHVAGSINIPLNEIPAKVDEIKAMEQ
                     PIILCCASGNRSGQATMYLQQFGIDCSNGGSWLMVNALV"
     gene            complement(1016..1225)
                     /locus_tag="PLL28_13725"
     CDS             complement(1016..1225)
                     /locus_tag="PLL28_13725"
                     /inference="COORDINATES: protein motif:HMM:NF039355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HNF70435.1"
                     /translation="MKKLKPYLSTIFGIAVGMLAGYVWYDYNACSTGTCPITSHPLNA
                     IIYGGVMGGLFFSSFKKKQKSNTIK"
     gene            complement(1227..2651)
                     /locus_tag="PLL28_13730"
     CDS             complement(1227..2651)
                     /locus_tag="PLL28_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNF70436.1"
                     /translation="MILEQIYTGCLAQGAYYLESNGEAAIFDPLREVQPYIDMANKRS
                     ATIKYVFETHFHADFVSGHADLAKKTGATIVYGPTKMETGFTAHIGTDGEIFKIGDIT
                     IKLLHTPGHTMESSCFLVHDEQGKPTAIFTGDTLFIGDVGRPDLAQHVIADLTQENLA
                     AHLYASLQEKILPLPDDLIVYPAHGAGSACGKNLSKETSDTLGNQKKFNYALRPGLTK
                     EQFISELLSGLTTPPSYFPKNVLMNIQGYDTIDTVLERGKKALSPDAFELIANETGAL
                     IIDTRKAADFASGFIPNSINIGHNGTFAVWVGTLVPDIKQEILIVAEKGREEEAITRL
                     ARVGYDHAIGFLDGGFDAWKNAGKEIDTIKTISVSEFIEAKNADNKIAILDVRKKSEF
                     DSEHIVEAENMPLDYINDYFTAIDKQKTYYVHCAGGYRSMIFASILRARGYNNLIDVD
                     GGFNAIKASGKFNITDYVCPTTML"
     gene            2863..3582
                     /locus_tag="PLL28_13735"
     CDS             2863..3582
                     /locus_tag="PLL28_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70437.1"
                     /translation="MRSIAIILMAAFSASPLMAQKDSGHNKLELHVGVVTSFTMPLGA
                     SDYDKSLFTTFIPAINYRGQVIYAHQLGLESSLHTGMGLGVSHYSFKLTMPSTGNSNR
                     SFKGSVACLDAQLPLYTKFTIAKGKHNSNYAMTAGAELHAVNIFSQGFTATSEWEETI
                     FEVSESDHIQLMATARLGIEGNHIGNNTFFGWCLLANYQVTPLGKMHMEATDLSGNSA
                     AGDLYPGSISLTAGVHIPLVK"
     gene            3610..>4156
                     /locus_tag="PLL28_13740"
     CDS             3610..>4156
                     /locus_tag="PLL28_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70438.1"
                     /translation="MKYILLLSTLFSLQLRGQTVIDTTIYSREQLIEDIRYCRDFHEK
                     HNTNLYAYRSKAYIDSMFDDLEDKVVPMDAITFFKYMSIVTSSISDGHCLLFPSEEFR
                     TTADTVKGFFPFRIHYDDGAFYVTMDLSDSENAFSNGKLPIEAINGIPADSIYHAALR
                     SLPREGNNLQYPNWILEKYFYE"
BASE COUNT         1184 a          889 c          794 g         1289 t
ORIGIN      
        1 ggtagcgaca gtatgccaaa attaatggct gatctgcaac caaaaattga ttcaatattc
       61 gacaaaacaa attatgaagt gcgcatcaca ggaactagtg ttgtggcatt aagcggatat
      121 caatatttaa tcgacagttt gttcgaaagt ttgttgattg catttattgg tatatcattg
      181 attctgcttt atcagttcag aagtttgcgc atgctagcca tttcattcct cccgaactta
      241 attccattat tcattacggg agccatcatg ggttttatgc atatagctat aaaacctagt
      301 accgtattgg tattcagcat agcttatggc atgtgtgttg atttcacttg ctactttatt
      361 gcaaaatata agcacgatct tatccgcaac agtttcaata ttccgaaaac cgtaatgagc
      421 agtttgcagg aagccggggt gagtatgata tacacttcgt tgattctgtt ctgcggtttc
      481 tttattttcg ttttcagcaa attcggcggc accgtgaata tgggtctgtt aacctctatc
      541 acactgctgg tggcgctatt caataattta ttattgctcc ccgctttaat gttgttgttt
      601 gaaaaaggat tgaacaaatc aaaccgcaaa ttcatcgaaa aatcaagtca cgaaaaggct
      661 tcccgtccgt ttacagattt gatcgatgaa catcagaaaa actgatcttt acgaaaataa
      721 aaaagtccga tagcggcgcc accggacttc aataagattg tcattaatta aactagcgca
      781 tttaccatca accagcttcc gccattggag cagtcgatgc caaattgctg taaatacatt
      841 gtcgcctgac cacttctgtt accactggcg caacagagaa tgatgggttg ttccattgcc
      901 tttatctcat ctactttagc aggaatttca tttaatggaa tattgatact ccccgccacg
      961 tgaccaccta taaattctgc aggtgtgcgt acatcgataa ttgttgtctg tttactcatt
     1021 ttatcgtatt tgatttttgt ttctttttga atgatgaaaa aaataatccc cccataacgc
     1081 cgccataaat tattgcattc aaagggtgcg atgtgatggg gcaagtgcct gtgctgcaag
     1141 cattataatc ataccataca taccccgcaa gcataccaac tgcgatgcca aaaatagtag
     1201 atagatatgg ttttagcttc ttcatattac aacatagttg tcggacaaac gtaatcagtt
     1261 atattgaatt tacctgatgc tttgatggca ttaaatccac catcaacatc aattaaatta
     1321 ttgtagcctc ttgccctcaa gatagaggca aaaatcattg aacgatatcc acctgcacaa
     1381 tgcacgtaat atgttttttg tttatcgata gctgtgaaat aatcattgat ataatctaac
     1441 ggcatatttt ccgcctcaac aatgtgctcg ctatcaaatt cacttttttt gcggacatca
     1501 agaatggcaa ttttattatc tgcattcttt gcttcgataa attctgagac tgaaattgtt
     1561 ttaatagtat ctatttcctt tccggcattc ttccatgcat caaatccgcc gtcaagaaat
     1621 ccgatagcgt gatcataacc tactcttgcc agacgggtaa tagcctcctc ttctctgccc
     1681 ttttcagcaa cgatcaaaat ttcttgcttt atatccggca ccaatgtccc aacccatact
     1741 gcgaatgtgc cgttatgacc aatattgata gaattcggaa taaatccact tgcaaaatcc
     1801 gctgcttttc ttgtatcaat aatcagggca ccggtttcat tagcaattaa ttcaaatgca
     1861 tctggtgaga gtgctttttt tccacgctcc aatacagtat ctattgtatc atatccctga
     1921 atattcatta atacattttt ggggaaataa gacggaggcg ttgtaagacc tgataacaat
     1981 tcgctgataa actgttcttt ggtaagtccg ggacgcaagg cataattgaa ttttttctga
     2041 ttacccaagg tgtcagatgt ttccttgctg agattttttc cgcatgcact tcccgctcca
     2101 tgtgccggat aaacaattag atcgtcggga agtggtaata tcttctcttg tagagaagca
     2161 tacaaatgtg cggcaaggtt ctcctgtgtc aaatctgcaa ttacatgttg tgcgagatcc
     2221 ggacgcccta catcgccaat aaacaaggtg tcgccggtaa atattgctgt gggttttcct
     2281 tgttcatcgt gcactaaaaa gcagctgctt tccatggtgt gtccgggagt gtggagcaac
     2341 ttgatagtga tatctccgat tttaaatatt tcaccatctg tgccaatatg tgcggtaaac
     2401 ccggtttcca ttttagtggg tccataaaca attgtcgcac ctgttttttt agccagatcg
     2461 gcatgtcccg aaacgaaatc ggcatgaaaa tgtgtttcaa aaacatattt aatggtagca
     2521 ctgcgtttgt tagccatgtc gatatatggc tgcacttccc tcagcggatc gaaaattgca
     2581 gcttcaccat tgctctccag ataatatgcg ccttgtgcca ggcatcctgt gtagatctgt
     2641 tccagtatca tagtttgtat tttatcatgt taatagatat acaaggtcga tgcagttgtc
     2701 ggctctctcc tgataggatg caaagatgtc gcaccaagca ttgccatatc gttacttttg
     2761 ttacacagtt acttttgtta caaaagcatc aatacttttc agaagcttgc catacgcaaa
     2821 ttttgaatta agcgttacct atctgtcaac aacatctacc ccatgagatc aattgcaatt
     2881 attctgatgg ctgcattcag cgcaagcccc ttaatggccc aaaaagattc cggacacaac
     2941 aagttggaat tgcacgtcgg agtagtaacg agctttacaa tgcccttggg cgcatctgat
     3001 tacgacaagt cattatttac cactttcatt cctgcgatca actatcgcgg acaagttatt
     3061 tatgcccacc agctggggct ggaatcttcg ttacataccg gaatggggct cggggtgtca
     3121 cattatagtt tcaagctaac aatgccatct accggtaaca gcaatagatc ctttaaagga
     3181 tcagtggcgt gtcttgatgc acaattgcca ttgtatacta agttcaccat cgctaaagga
     3241 aagcataaca gcaattatgc catgacagcc ggtgccgaat tacatgcagt gaatattttt
     3301 agtcagggat tcacggcaac atcggaatgg gaagaaacga tattcgaagt ttcggaaagc
     3361 gatcacatac aattgatggc aacagcccgc ttaggaattg agggaaatca tatcggaaat
     3421 aatactttct tcggctggtg tctactggca aattatcagg tcacaccgct tggcaagatg
     3481 catatggaag caaccgacct ttccggcaac tcagcagccg gagatctata tccaggtagc
     3541 atttcactta ctgcaggcgt gcacattcca ttggtcaaat gatttaatat ctttgatcac
     3601 tacatgtcca tgaaatacat tttactcctc tcaaccttgt ttagcctgca attgcgtgga
     3661 caaaccgtta tagacacgac catttattcg cgagagcaat taattgagga tattcgctat
     3721 tgtcgcgatt ttcacgaaaa acacaatacc aatttatatg cctatcgttc aaaggcatat
     3781 atcgacagca tgttcgatga tctcgaggat aaagttgtgc caatggatgc cataactttt
     3841 ttcaagtata tgtccattgt tacatcttcc atttcagatg gtcactgctt attatttcca
     3901 tccgaggaat tcagaacaac agcagatact gtgaaagggt tctttccatt tcgcatacat
     3961 tatgatgacg gtgcatttta tgttactatg gacctttcag acagcgaaaa tgcattttca
     4021 aacggcaagt tacctattga ggccattaat ggtatacctg cagattccat ctatcatgct
     4081 gcactgcgct cgctgccgcg ggaaggaaat aatttacaat atccaaattg gatcctcgaa
     4141 aaatattttt acgaat
//
LOCUS       DAOQHY010000239         4152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_12882_length_4152_cov_4.559922, whole genome shotgun sequence.
ACCESSION   DAOQHY010000239 DAOQHY010000000
VERSION     DAOQHY010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12882_length_4152_cov_4.559922"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1900
                     /locus_tag="PLL28_13745"
     CDS             <1..1900
                     /locus_tag="PLL28_13745"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNF70439.1"
                     /translation="TDEVTNATVASYDVSDLTDIKELDQFQANPGTNSIPHNVMVLGD
                     FLVTAYYRDGVVITDATYPDNMIKVGEYDTSPFAGDGFNGAWGSWPYLPSGNIIVSDM
                     EEGLFIVGPTYVKACYVEGNVTDASTGAPIFGATVNFTGVVDATSDLIGDYHGGVLDA
                     GTYDFTFNKTGYLPETVTGVVMENGIVAIVDAELTPLPSYTITGQVLDAATGAGIAGA
                     DVLFQNALYNFSGTADASGNFSFDMYEDDYDFIAGDWGYVTNGINVTVSAGGTYTITL
                     SKGYYDDFIFANDWTTSGTTDVGMWERGEPNGTFAGPDASNPDFDVTDDYGDQAYVTG
                     NEPGGTVGTDDVDNGIVNLISPSMDLTGYADPVLSYDRWFFNGGGTGTPNDTLRIFVS
                     NGIEEVLIDAINSSSTELSEWVNVEVNLSTYIDITSDMHVRFRTADQSASGHLVEAGV
                     DLFMVYDNITSEPTTAFGSDVTTGCGPLTVVFDDMSVGATSWSWTFPGGTPATSTLQN
                     PTVVYATPGTYDVTLVASNIVGESESTTTGYVTVNLCNAIDEVSPAGFTVSPNPFNTL
                     FSVEIEAANATDIRIVDITGRIIRTLQTNGEQVMVIAADQLPAGIYTIELLKNNEVIG
                     TTKAVKAE"
     gene            2105..>4152
                     /gene="secD"
                     /locus_tag="PLL28_13750"
     CDS             2105..>4152
                     /gene="secD"
                     /locus_tag="PLL28_13750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNF70440.1"
                     /translation="MQGKGLVKFFLAAMILVTIYQLSFTLVASRESKKADEAAQAAVD
                     PTLKGQARTEAISTAKSDYLAQNADKIVYNIGIAKYNYDDVQKRAINLGLDLQGGMSV
                     VMEVSKYDLMKQLTSDPKNQKFIKALDQARLQEAQGQGDLIELFADNYLTANPGAQLV
                     TLFANSSNVDDLPRNSDNDAVISWLKKSADDGVESTYTKLKERIDKFGVTQPYVTLQK
                     STGRIMVELPGVDNPKRARELLQSTANLQFWGVWRYEEVGGAINQANNVLTQLEDIEA
                     PVSGDTGVTENTSPVDSPAVAATTPAGDDSLAANDDTLLADNEEAQTEDLGPLSSLLL
                     ERSDPAAIGMVAEDDTFKLMNYFRMEEVANVFPEGTKFMLSAKPAKGSRNYDVYAIKS
                     NGDDDQPLLDGSVVKNASPDRDQVQNIVISLSMNTEGAQIWKKVTEANVGNFIAIALD
                     NKVYSAPRVNEAIPSGRSSISGSFTAEEAQDLSNILEVGKLPVPSRIVQEESVGPSLG
                     AESIRVGLLSMIAGLILVIIFMVIYYSTAGMIADIALFANIFFIFGALASMGATLTLP
                     GIAGLVLTMGMAVDANVIIYERIREELDKGKSMIKAVADGYSGSYSAIIDGNLTTLII
                     AIILMNFGTGPVKGFAVVLTIGIIASLFSSILISRLIIDAMVKKDRPVKYYTSFTKHA
                     "
BASE COUNT         1164 a          962 c          908 g         1118 t
ORIGIN      
        1 taccgatgag gttaccaatg ctaccgtagc ttcttacgac gtttccgacc ttaccgatat
       61 caaagaactt gatcaatttc aggctaatcc cggaaccaac tctattccgc acaacgtaat
      121 ggtcctcggc gatttccttg taaccgctta ttaccgcgat ggcgttgtga ttacagacgc
      181 gacatatcct gataacatga ttaaggtcgg cgaatacgac acctctccat tcgccggcga
      241 cggattcaac ggcgcttggg gttcatggcc ttacctccct tccggaaata ttatcgtttc
      301 cgatatggaa gaaggcttgt tcatcgttgg accaacttat gtgaaagcct gttatgtgga
      361 aggcaatgtt actgatgcgt ctaccggtgc tccgatattt ggcgctactg tgaactttac
      421 aggagtggta gatgctacgt ccgacctcat aggcgactat cacggtggtg tgcttgatgc
      481 cggaacctat gatttcactt tcaataaaac cggctatcta cccgaaactg taaccggtgt
      541 agttatggaa aatggcattg ttgctattgt tgatgcagaa ctgacaccac ttccatctta
      601 tactataaca ggtcaagtac tggatgccgc aacaggcgca ggaattgcag gagctgacgt
      661 gcttttccag aatgcactct ataacttctc aggaactgcc gacgcttccg gtaacttctc
      721 atttgatatg tatgaagatg attacgactt catagcaggt gattggggct atgtaacaaa
      781 tggcattaac gtaactgttt ctgcaggcgg aacttacacc atcaccctca gcaaaggata
      841 ttatgatgat ttcatttttg ctaacgactg gactacctcc ggaactaccg atgtaggtat
      901 gtgggaacgc ggcgaaccga atggaacttt tgcaggtcct gatgcctcaa atcctgactt
      961 cgacgttact gacgattatg gtgatcaggc ctatgttacc ggaaatgaac cgggcggaac
     1021 ggtaggaacc gacgatgtag acaatggcat tgtaaacctt atttcacctt caatggacct
     1081 caccggttat gctgatcctg tgctctctta cgaccgttgg ttctttaatg gcggcggaac
     1141 aggaactcca aacgacacac ttcgcatctt tgtaagcaac ggcattgaag aagtattgat
     1201 agatgctatc aacagttctt ctaccgaact cagcgaatgg gtgaatgttg aagtaaactt
     1261 atcaacctat attgatatca cctccgatat gcatgttcgc ttccgcacgg cagatcaatc
     1321 tgcttccgga caccttgtag aagcaggtgt agacctgttt atggtttacg acaatatcac
     1381 cagcgaacca actactgctt ttggctctga tgtgaccacc ggctgcggac cgttaactgt
     1441 tgtgttcgat gacatgtcgg ttggtgccac ctcctggagc tggaccttcc ccggcggaac
     1501 acctgctaca tctacgcttc aaaaccctac cgtagtatac gcaactcccg gaacttatga
     1561 tgttacgctg gttgcctcaa acattgttgg cgagtcagaa agcactacaa ccggatatgt
     1621 taccgttaat ctctgtaatg ctatagatga agtatcacct gccggattta cagttagccc
     1681 aaatccattt aacaccttat tctctgttga aatcgaggct gcaaatgcaa cagatatccg
     1741 catcgtcgat atcaccggtc gcattattcg cacgcttcaa acaaatggcg aacaagtgat
     1801 ggttatcgct gcagatcagc tccctgcggg catttacacg atagaactgc taaaaaacaa
     1861 cgaagttatt ggtacgacca aagcggttaa agccgaataa tacaaagccc ttcaaatcat
     1921 tcagaaatgc cgctttatga gcggcatttc tgttttaggg agtttccata tcgcaaagcc
     1981 ctgaaaaaca tagcatcaga gtagaaaact tcacacgttc accctatatt tgcagccgcc
     2041 tttgacgaaa aaggtgtgcc cgtattatgt acaggcctaa aatcaaaaca caacaattac
     2101 agttatgcag ggaaaaggtt tagtaaaatt ctttctggca gcgatgatcc tcgtgaccat
     2161 ctaccaatta tcattcacac tcgttgctag ccgtgaaagc aaaaaagccg acgaagcagc
     2221 ccaagccgca gttgacccga ctttaaaagg tcaggcgaga acagaagcaa tttctacagc
     2281 aaaaagcgat tacctggcgc aaaatgccga caaaatcgtt tacaacatcg gaattgcgaa
     2341 gtacaattat gatgatgtgc aaaaacgtgc catcaacctg ggtctcgatc ttcaaggcgg
     2401 tatgagcgtg gtaatggaag tatcgaagta cgatctgatg aaacagctta cttctgatcc
     2461 caaaaaccaa aaattcatta aagcactcga tcaggctcgt ttacaagaag ctcaaggaca
     2521 aggcgatctt attgaattgt ttgccgataa ttacctcaca gcaaatccgg gtgcacaatt
     2581 agtgacctta tttgcaaaca gctcaaatgt tgacgatctg ccgcgcaact ctgataacga
     2641 cgctgtaatc agctggttga aaaaatctgc tgatgatggt gttgaatcaa cttataccaa
     2701 actcaaggaa cgtatcgaca aattcggcgt tacccagcct tatgttaccc tgcaaaaaag
     2761 caccggtcgc atcatggttg aattgcccgg tgtcgacaat ccaaaacgtg cccgcgaact
     2821 cctccaatca acagcaaacc ttcaattctg gggcgtttgg cgttatgaag aagttggtgg
     2881 cgctatcaac caggcaaata atgttttaac tcagctagaa gatattgaag cccctgtttc
     2941 cggcgatact ggagttacag aaaatacttc acctgtagat tctccggcgg ttgcagcaac
     3001 aactcctgcc ggtgatgaca gtttagctgc taatgatgac acacttttag cagataatga
     3061 agaagctcag actgaagacc tcggcccttt gtcatcatta ttattggagc gttcagatcc
     3121 ggcagctatc ggtatggttg ctgaagatga caccttcaaa ttgatgaact atttccgtat
     3181 ggaagaggtt gccaacgttt tccctgaagg taccaaattc atgctgagtg ctaaaccggc
     3241 aaaaggttca agaaattatg atgtatatgc tatcaagagc aacggtgatg atgatcagcc
     3301 actgcttgac ggtagcgtag tgaaaaatgc ttctcccgac cgtgatcagg ttcagaatat
     3361 agtgatctcg ctgagtatga acaccgaagg tgcgcagatt tggaaaaaag taaccgaggc
     3421 taacgttggt aattttattg ccatcgcact tgataataaa gtatattctg ctccacgcgt
     3481 aaatgaggct attccaagcg gtcgctcttc aatctcagga agctttaccg ctgaagaagc
     3541 tcaagacctt tcaaacattc ttgaagtagg taaattaccc gttccttctc gtatcgtaca
     3601 agaagaatcg gttggacctt ctttaggtgc agaatccatc cgtgtaggtt tattgtcaat
     3661 gattgcaggt cttatactgg taatcatttt catggtgatc tactactcta ctgccggtat
     3721 gatagctgat atcgccttgt ttgcgaatat cttcttcatc ttcggtgcac tggcatcaat
     3781 gggtgcaaca ctcacattgc cgggtatcgc aggtctcgta cttaccatgg gtatggctgt
     3841 ggatgcgaac gtaatcattt atgaacgtat tcgcgaagaa ttagataaag gtaagagcat
     3901 gattaaagca gttgccgatg gttattcagg atcttattcc gccatcatcg acggtaactt
     3961 gactaccctc atcatcgcca tcattctgat gaatttcgga acaggtccgg tgaaaggatt
     4021 cgcagtagtg cttaccatcg gtatcatcgc atctctcttt tcatccatac ttatttcccg
     4081 cctcattatt gatgcaatgg tgaagaaaga tcgccctgta aaatattata ccagcttcac
     4141 aaaacatgct tt
//
LOCUS       DAOQHY010000240         4139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_12946_length_4139_cov_4.851861, whole genome shotgun sequence.
ACCESSION   DAOQHY010000240 DAOQHY010000000
VERSION     DAOQHY010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12946_length_4139_cov_4.851861"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            360..2363
                     /locus_tag="PLL28_13755"
     CDS             360..2363
                     /locus_tag="PLL28_13755"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70441.1"
                     /translation="MTKRNEIGIFGMPLNSMVLRLLFVLIWIILSYRIATAQTISSFA
                     PTTGPTGTVVTITGTGFDNTITNNVVFFGATQATVSSATITSLVVTVPASASYQYITA
                     TNLVTNLTAYSLQPFLYSDLCNSATNFASAVSVQGGSHNVEIYDMDGDGLPDLIIPDY
                     FNNYFTVTRNTSTPGTISFAPKVTFAWASGGQPNEVSIGDFDGDGKPDVALGGNGGGS
                     FVSVYRNTSTPGSISFAARQDFTASSGQGNGNRIIDIDGDGKPDLLSVHWSGNCLWVL
                     RNTSTGPGVISFAAGVSFSTTTQPHKIDVGDLDGDSKPDVVITAFTSNKMSVFRNTST
                     VGTISFAARQDFSPGTVPYFVHLGDLDGDGKLDVALVNRDVATMAVYRNTSTVGTISF
                     ATKQDFATPAGTFTHYGVGLGDIDGDGKLDAVAGNYTLNVTSIFRNTSTVGAISFATR
                     VDVASGVSYDVGIDDFDQDGLPDIAVVNSGTTVAILRNQCLLLPIELTDFTGSQQGHA
                     VKLEWTTASELNNDKFIIERSSNGDDFDAIGIVSGAGTTEIVHNYQFFDQAPAGGINY
                     YRLKQVDLDGAFTYSQMIVVEVSGEASYLKIYPNPSNGLCTIMNTDGSSEIVMLNELG
                     EVVYTITGNANITHINIGQLPAGMYTVVQITNGAYRSGKLIKY"
     gene            complement(2376..3209)
                     /locus_tag="PLL28_13760"
     CDS             complement(2376..3209)
                     /locus_tag="PLL28_13760"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70442.1"
                     /translation="MKRLLIALLITATGSLKAQFTLEHTYDSAAAYNFCAKGLSQLLY
                     VDLEASGEHYVRINRCGELISLYDMDHNLVKNISMSMLHTDPPYNVIGSIMYISEHLF
                     NLDDRLEFMYVVDSGDYYITNVYNEIGEVLFSEPGMPSVVLNFHMQQFPIYNTDQGTK
                     LILSYQNNQAKVFGLEGTLTTAIEVANQELTAGAALAAAYPNPADNISYIPYQLPEGE
                     TTGKIIIYAMNGSEVMRFDVSSADSGLTIPTNTLTAGTYFYALETATSLVSGKQLVVV
                     N"
     gene            3370..3627
                     /locus_tag="PLL28_13765"
     CDS             3370..3627
                     /locus_tag="PLL28_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70443.1"
                     /translation="MSTTLETLIAFLSIAVILFLIFFGDNIKKNIYFNIVILMIVSCF
                     IGYQFSNLHEQSQWYSIALIGVMGMGLINVLGRIGKRMKEE"
BASE COUNT         1208 a          732 c          982 g         1217 t
ORIGIN      
        1 aatcttggcc agttaataac cactcaaatg gcatcaggaa caagtgtaac ctcaattgat
       61 gttacaaatg ttgcagccgg tatgtatacc ataatggcta ctgatgaaaa ccatgttgct
      121 gctcaggcgg taattattga ataattatat tatcacacat tgcaatgcca tcctccgggg
      181 tggcattttt tttgtggcga atcgcctgtt tttgttgtta aaaattactg caaagtgagg
      241 aaagtcactt gattgccatt gattttgttg tctatttgct tatggatagg catttattgt
      301 cagttccgga atttagttcg gaaattgaaa tatggtcacc ttgaccctgt ccttggttga
      361 tgacgaaacg caatgaaatc ggaattttcg ggatgccgct taacagcatg gtattgagat
      421 tgctatttgt acttatctgg attatattaa gctaccggat cgcaacagct caaacaattt
      481 cttccttcgc acctactacc ggtcctacgg gtactgtggt gacaatcaca gggacaggtt
      541 tcgacaatac tatcactaac aatgttgtct tttttggtgc tacacaggca acggtgagca
      601 gtgctacaat aacgagttta gtggtcactg ttccggcgag tgcaagctat cagtatatta
      661 cggctacaaa tcttgttacc aatctcacag catattcact gcaaccattt ttatattccg
      721 atttgtgtaa tagtgcaacc aattttgcat cggcagtgag tgtgcaggga gggtcgcaca
      781 atgttgagat ttacgatatg gatggagatg gattaccgga cctgattatt cctgattatt
      841 ttaataatta ctttaccgta accagaaata cctccacacc gggaaccatt tccttcgcac
      901 ctaaagtaac gtttgcatgg gcttccggcg gacaaccaaa cgaagtgtcg ataggcgatt
      961 ttgatggcga tggaaaaccg gatgtggcat tgggtggaaa tggcggcggt tcttttgttt
     1021 ctgtatatcg caatactagt acgcccggca gtatttcttt tgctgcgcga caagatttta
     1081 cggcttcatc agggcaggga aatggaaata gaataattga tatcgatggt gatggaaaac
     1141 ctgatttatt atctgtgcat tggtcgggca attgtttgtg ggtattgaga aatacatcta
     1201 caggtcccgg cgtaatttct tttgcagcag gcgtaagttt ctccacaaca acacagccgc
     1261 ataaaattga tgtaggcgat ctggatggtg atagtaaacc cgatgtagtg attacggctt
     1321 tcaccagtaa taaaatgtcg gtgttcagaa atacgagtac ggtaggaaca atttcttttg
     1381 ctgcgcgaca agatttttcg cccggtacag taccttattt tgtgcatctg ggcgatctag
     1441 atggcgatgg aaaattggat gtcgccttag taaacagaga cgtggcaacc atggcagttt
     1501 acagaaatac cagtaccgtt ggaacaatat cctttgccac gaaacaagat tttgcaacac
     1561 ccgcaggaac cttcacccac tatggagttg gacttggtga tattgatggc gatggaaaac
     1621 tggatgcggt agctggaaat tatacactga atgtcactag tatttttcgc aacacaagta
     1681 ctgtaggtgc catatctttt gctacgcgtg tagatgtagc ttcgggagta tcatacgatg
     1741 taggcatcga tgattttgat caggatggat tacctgatat tgctgtagta aatagtggta
     1801 caacagtggc aattttacgt aatcagtgtt tattgcttcc aatagagttg actgatttca
     1861 ccggaagtca gcaaggccat gctgtaaaac tggaatggac aactgcttca gaattgaata
     1921 atgataaatt cattattgag cggagcagta atggtgatga ttttgatgct ataggcatag
     1981 tgtcgggagc gggcaccacc gagatagtgc ataattatca attttttgat caggcacctg
     2041 caggtggtat caattattat cgtttaaaac aagtcgacct tgatggtgcg ttcacttatt
     2101 cgcaaatgat tgttgtagaa gtaagcggtg aagcatccta tttaaaaata tatcctaatc
     2161 catctaacgg cctgtgcacc atcatgaaca cagatggttc gagcgaaatt gtgatgttga
     2221 atgagttggg tgaagtagtc tatactataa caggtaatgc aaatatcact catattaata
     2281 ttggtcaact accggccggc atgtatacgg ttgtgcagat cacgaatggt gcatataggt
     2341 caggtaaact tatcaagtat tgatcgcgta aattgttagt tcactaccac caattgtttt
     2401 ccggatacca gggatgttgc tgtttccaac gcataaaaat aggtgcctgc agttaaagtg
     2461 tttgttggaa tggtaagtcc gctatctgcg ctgctgacat caaaacgcat cacttctgaa
     2521 ccattcattg catagatgat gatcttacct gttgtttccc cttcgggaag ttgatatggg
     2581 atataactga tattgtctgc cggattaggg tatgccgctg caagtgctgc tcccgctgtg
     2641 agttcttgat tggcaacttc tatggcggtg gtcagtgtac cttcaagtcc gaataccttc
     2701 gcttgattgt tctgatagct gaggattaat ttcgtgcctt gatcggtatt gtagatagga
     2761 aattgttgca tgtggaaatt gagtactaca ctgggcattc cgggctccga gaaaagcact
     2821 tcacctattt cgttatatac attcgtgatg taataatcac cggagtcaac tacatacatg
     2881 aattccaatc tgtcatccag attgaacagg tgttcagaga tatacataat ggaaccaatt
     2941 acattgtaag gtgggtcggt atgtagcatg ctcattgata tgttctttac aaggttatgg
     3001 tccatatcat acaaactgat taactccccg cagcgattaa tccgcacata gtgttcaccg
     3061 gaggcttcaa gatctacata caacaactgg ctgaggccct tagcacagaa attataagca
     3121 gcagcactgt cgtaagtgtg ttcgagggtg aattgggcct tgaggctacc ggtagcagtt
     3181 atcagcaatg cgataaggag tcgtttcatt gtggcaggtt tagtgaaatg gtttgctaag
     3241 taaatatagt gaattgttgg gttgaatagg gggaggagcc taagatgggt aaataagtga
     3301 ttggatgtga taaatagctg cagaataatt ggtcaagatt ttgtaccttt aatttcaaat
     3361 ctgatctata tgtcaaccac tctagaaaca ttgatagcat tcctatctat cgcggtcatc
     3421 ctgtttttaa ttttttttgg agataacatc aagaaaaaca tctatttcaa tattgtgata
     3481 ttgatgatcg tgagctgctt tataggatat caattttcaa acctgcatga gcagtcgcaa
     3541 tggtattcaa tagcgctgat aggagtgatg ggaatgggat tgataaacgt gttgggaaga
     3601 ataggaaaaa ggatgaaaga ggaatgagaa aggaaagagg agaaaggaaa gaggagaaag
     3661 gaaagaggag aaaggaaaga ggagaaagga aagaggagaa aggagagagg agatgcaata
     3721 catgaatttt caataaatga aaaaccactc tggaaggaag tggttttttt cttttgtgat
     3781 ttcgccggga cttcctggca agactacgca acggtatttg gacctaggat tttatcgaat
     3841 ctttgttttt cgtaaaagag ggcaaactgc tgtagtcagg ttatagactg accaaaaacg
     3901 aaatgaagta aggttaaaac ctgacaaaaa tttgtaaaag tcagtctaaa accttacttt
     3961 tggtaaaaat cctagagtta tggacaagca aagcctatta ttacaacagt tgaatgcaag
     4021 aatggcggca tttgccgaat tgaagcaggt agcagttccc tccactgggt ggataaaagc
     4081 gatccgcagt gctttgggca tgtcattaca gcaactgggg aagcgcatat ctataacga
//
LOCUS       DAOQHY010000241         4122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_13031_length_4122_cov_6.078190, whole genome shotgun sequence.
ACCESSION   DAOQHY010000241 DAOQHY010000000
VERSION     DAOQHY010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13031_length_4122_cov_6.078190"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PLL28_13770"
     CDS             <1..667
                     /locus_tag="PLL28_13770"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF70444.1"
                     /translation="SLIVPNPDLGPEKTINADLSIVKVFKDKLEIELTGFYTRFTDYI
                     ALEPGTFEGADSIEYDGVLSEVLTTVNKGSAYLYGFNVGADVNIINGLNLSSYITYTY
                     GRIETDTTPYPLDHIPPVYGRTGLKYAKKKLVAEAYALYNGWKYIEDYNIVGGEDNDQ
                     YATPEGMPSWYTLNIKASYDVMEYVTIQAGCENLMDHQYRVFASGISAPGRNIFVAVR
                     AKF"
     gene            790..1092
                     /locus_tag="PLL28_13775"
     CDS             790..1092
                     /locus_tag="PLL28_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70445.1"
                     /translation="MSLFSKLLGGNKGGGGGNKAQAFITQLTQDLHLSSDQVTQVEAA
                     LREFFSEKREMKQSGNKDGFKESKQDFKDDILKALSPEQQQKFMDNIQTYKQLLRK"
     gene            1145..1675
                     /locus_tag="PLL28_13780"
     CDS             1145..1675
                     /locus_tag="PLL28_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70446.1"
                     /translation="MTDLLFANVLLFGSNEPTVHLPEGLYKALLASHSGLRYIVMILL
                     ILAVVYARQTSSGKKPFAGATKKMGMFTMILVDIQLLVGAALYFNFIASQTNFKLGKL
                     KDQLSAPMFRSIALDHALGMFIAIVLIHLGYAKAKKSMNDVDAGKKQFMFYLIALILI
                     LVSIPWPFLHHERGWF"
     gene            complement(1746..2378)
                     /locus_tag="PLL28_13785"
     CDS             complement(1746..2378)
                     /locus_tag="PLL28_13785"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNF70447.1"
                     /translation="MDEAEYISQAKTCALYKKGQTIFHEHANSYGLYCLNEGKVKLYK
                     QAPDGKEQIMKIVTPGDFIGYGAMLSSQTYGVSAEVIEDAVICFIPKDSVLKIFRENN
                     RFSEGMVQLLTKTIDQTVEKMADIAYKPVRGRVAEALLLLSESYKTEDNPDGVVSITR
                     EDLASYVGTVKETAIRVLKDLKEEGLIETNNHAIKIIDPKGLAKVCELYD"
     gene            complement(2494..3417)
                     /locus_tag="PLL28_13790"
     CDS             complement(2494..3417)
                     /locus_tag="PLL28_13790"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNF70448.1"
                     /translation="MPSSINGPGKQESSRTRTQKREQFKERLMQVNRILVPTDYSENA
                     DLALVLAVDIAKQFDAELLVVHAYHMPFTDEHMPPDMVKELLAAQKLRADDDMQRYAG
                     KHRTDLKWTKVQFKAMMGFASETILDIAHTEKVDLIVVGTQGANSIEDRMFGTVTWNI
                     IKKSDIPVIALPTHNEPVSIKNVMVPFEGTDQDYDIIGYLLGFTAKYNATLHAVHFMN
                     DTNSYNKALVDKLQTGFRKEIDAERLQLHFLAEKNITEGIKKFASRNQVDMIGMVTHN
                     HGLFSTIFHMSVTRNIALYSQIPLLAYNCDK"
     gene            complement(3652..>4122)
                     /locus_tag="PLL28_13795"
     CDS             complement(3652..>4122)
                     /locus_tag="PLL28_13795"
                     /inference="COORDINATES: protein motif:HMM:NF026080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4468 domain-containing protein"
                     /protein_id="HNF70449.1"
                     /translation="AFPVDEETKLVTYTDVIQVPGITKDSLYNVAMEWMKKFYKSPSQ
                     AIKSQSKEDGVIEIKHQFQLTRDEKGQTVKAAQINYYLTLQFRDGRFKYTITKIHVEG
                     PSYFGIEKWINEENFAKDENVTGYLVQIEDFMNKLTESLDSELRPAAPKKEEEW"
BASE COUNT         1146 a          900 c          809 g         1267 t
ORIGIN      
        1 aagtcttatc gttccaaatc cagatcttgg ccctgaaaaa acaattaacg ccgacctttc
       61 tattgtgaag gtgtttaaag acaaactcga aattgagctt accggttttt acacgcgatt
      121 taccgattat atcgcattgg aacccggaac tttcgaagga gccgattcaa ttgaatatga
      181 tggcgtgttg agcgaagtac ttacaacagt aaacaaaggc agtgcttacc tctatggatt
      241 taatgttggt gccgatgtaa atatcatcaa tggactgaat ttgtcatcat acattacata
      301 cacatatggt cgcatagaaa ccgataccac tccatatcca ctcgatcata ttccgccggt
      361 ttacggaaga acaggcctca aatacgctaa aaagaaactt gttgccgaag cttacgccct
      421 gtataacgga tggaagtata ttgaagatta taacatcgtt ggtggtgaag ataacgacca
      481 gtatgctaca cctgaaggaa tgccttcatg gtataccctc aatatcaaag catcatacga
      541 cgtaatggaa tatgttacca tccaggcagg ttgtgaaaac ctcatggatc accaatatag
      601 agtattcgcc tccggaattt ctgctccggg tcgcaatatc tttgttgcgg tgcgcgcgaa
      661 attctgataa aaagaacccg cccctgtaag tctgacctca ggatactgcc cccggagccc
      721 tgatgtcaac accacagggg cgtggtgttt tcgtgaaaag tgataacttc acagcctaaa
      781 tatgaaacaa tgagcctctt ttcaaaactt cttggcggca ataaaggcgg tggcggaggc
      841 aacaaagccc aggcttttat cacccaactg acccaggacc tgcatctttc aagcgaccag
      901 gttactcagg ttgaagcagc gttgcgtgaa ttcttttccg aaaagcggga aatgaaacaa
      961 tccggtaaca aagacggatt caaagaaagc aagcaggatt tcaaagatga tatcctcaaa
     1021 gctttaagtc ctgaacagca acaaaaattt atggataata tccagactta taaacaattg
     1081 cttagaaagt aaatcttttc aaggcaattg aaatcagttt ttatttaacc acaaaccaca
     1141 ccacatgaca gatttactgt tcgcaaatgt attgttgttc ggttctaatg agccaacagt
     1201 gcatcttccc gaaggattgt ataaggcctt attggcatcg cactccggct tgcgttatat
     1261 tgtaatgatt ttattaatcc ttgcagttgt ttacgctcgt caaacttcat ccggaaaaaa
     1321 acctttcgcc ggtgctacta agaaaatggg tatgttcacc atgatcttag tggatattca
     1381 attattggta ggagctgcac tatatttcaa ttttatcgca tctcaaacaa attttaaatt
     1441 gggaaaattg aaagatcagc tgagcgctcc aatgtttcgc tcaatagcgc tggatcatgc
     1501 gctgggaatg tttatcgcaa tcgtgctgat tcacttaggg tatgctaaag cgaaaaaatc
     1561 tatgaatgat gtagatgcag gaaaaaaaca atttatgttt tacctgattg cacttattct
     1621 gatactcgtt tctattccat ggccattcct gcatcatgaa cgcggatggt tctgataact
     1681 gaaaaattgt acataaaaaa cgggtctgtt acaacggacc cgttttttat gcagtttggt
     1741 ttctgttaat cgtacaattc acaaactttt gccaatcctt tcggatctat aattttgata
     1801 gcatgattgt ttgtttcaat caatccctct tccttcaaat cttttaatac acgaattgca
     1861 gtttctttta ccgtaccaac ataagacgca aggtcttcac gtgtaatact cacaacgcca
     1921 tccggattgt cctcggtttt ataagattca cttaatagta acaacgcctc cgcaacgcga
     1981 cctcttaccg gtttataggc aatatcggcc attttctcaa cggtttgatc aatggtcttg
     2041 gtcagcagtt gcaccatgcc ttcagaaaag cgattgtttt cacggaatat cttcaacacg
     2101 gaatctttcg gtataaagca tatcacggca tcttcaatta cctcggcact cacaccgtaa
     2161 gtttgagagc tcaacatcgc cccatatccg ataaaatcgc ccggcgttac aattttcatg
     2221 atctgttcct tgccatccgg agcctgttta tagagtttta ccttgccctc attgagacaa
     2281 tacaacccat atgagttggc gtgctcgtgg aatatggtct gccctttttt atacagggca
     2341 caggtcttag cctgactgat atattccgct tcgtccacat gacaaaagtg gaacaacgtt
     2401 ttgttcctgc tcttgcattg ggcgcactcg ggggtttgaa ttcgtgattt cgtggtcata
     2461 atttttgaat ttgcctgaat tgtcataaaa ttatcattta tcgcaattgt atgcaagtag
     2521 tggtatctga ctgtataacg caatattgcg tgttacactc atatggaaga tggtagaaaa
     2581 cagtccgtga ttgtgtgtca ccatgcctat catgtctacc tgattcctcg aagcaaactt
     2641 cttgatgcct tccgtaatgt tcttttcagc taaaaaatgc aattgcaatc gttcggcatc
     2701 aatctctttc ctgaaaccgg tttgcagctt gtctaccaat gctttgttat atgaattggt
     2761 atcattcatg aaatgaacag catgcaacgt ggcattgtat ttagcggtaa atcctaacaa
     2821 ataaccaatg atatcataat cctgatcggt gccttcgaac ggtaccataa cgtttttaat
     2881 agaaaccggt tcattatgag taggtaaggc gatcaccgga atgtcagatt tcttgatgat
     2941 attccatgta acagtgccaa acatcctgtc ctcaatacta ttggccccct gggttcctac
     3001 tactatcaag tctacttttt cggtatgggc aatgtccaag atggtttctg acgcaaaccc
     3061 catcattgcc ttgaattgga ctttagtcca ctttaagtcc gtacgatgct taccggcata
     3121 gcgctgcata tcatcatcgg ctctcaactt ttgagcggca agtaattctt ttaccatatc
     3181 cggtggcata tgctcatcgg taaacggcat gtgataagca tgcacaacta ataattcggc
     3241 atcaaactgc ttggcaatat caacagccaa aaccaacgcc aaatcggcat tttcggagta
     3301 gtcggttgga accaaaatgc gatttacctg cataagtcgc tctttgaact gttcacgctt
     3361 ttgtgttcgc gttctgctcg attcttgctt gcccggtcca tttatcgagg acggcatgct
     3421 gttaagttgg tcgtcagtca ttggaaagat ttttttactg attcaaaatt gaacatagtt
     3481 cacccgaaac agcatgatat acctcaagcc aaatggtgac ttttgtcaat ccaaaaacaa
     3541 aaaacctgcc tgaaaagctg tttcaggcag gttttttttg gggtttgaag tagtatccgg
     3601 ttgtctgtat ccggattttg ccttcctacc gcacaatttc gatgatttat tttaccattc
     3661 ttcctctttt ttaggagcgg cagggcgtaa ttcagaatcg agactttcag tgagtttatt
     3721 cataaagtcc tctatctgaa ccagatagcc ggtaacattc tcatcctttg caaagttttc
     3781 ttcgttgatc cacttttcaa ttccgaagta ggaaggacct tcaacatgta tcttggttat
     3841 tgtgtatttg aacctgccgt cgcgaaactg caatgtcaga taataattga tctgcgcagc
     3901 ttttacggtt tgtccttttt cgtcgcgcgt aagctgaaac tgatgcttga tctcaatcac
     3961 gccatcttct ttgctttggc ttttgatagc ctggcttgga ctcttataga atttcttcat
     4021 ccattccata gctacattgt acagtgagtc ttttgtaatg ccgggaactt gaataacatc
     4081 ggtataagtt accagttttg tttcttcgtc gacaggaaat gc
//
LOCUS       DAOQHY010000242         4101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_13139_length_4101_cov_4.461691, whole genome shotgun sequence.
ACCESSION   DAOQHY010000242 DAOQHY010000000
VERSION     DAOQHY010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13139_length_4101_cov_4.461691"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PLL28_13800"
     CDS             <1..589
                     /locus_tag="PLL28_13800"
                     /inference="COORDINATES: protein motif:HMM:NF024490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNF70450.1"
                     /translation="LVAPINLRSRLKLLINREIKFAKEKKKAAIFLKVNNLVDKEIIK
                     KLYEASMAGVQIKLIVRGACSIVPGVEGLSDNIEAISVLDRFLEHPRVFAFHNNGKEE
                     VFIGSADIMERNLDHRIEVMVQVLDKDIRKMIKDILTIQWGDNEKARLLGHGHLNDYV
                     KVLPGEKQVRSQMALYDYFEKSRLASALEKKEGNK"
     gene            592..1488
                     /locus_tag="PLL28_13805"
     CDS             592..1488
                     /locus_tag="PLL28_13805"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HNF70451.1"
                     /translation="MKFAAIDIGSNAVRLLIENVYETSSGPVFHKDSLIRIPVRLGED
                     AFLDGAISEEKVQMLVKAMVAMRHIIDIHKVTAIKVAATSAMREAANSGDIINRVMEA
                     SGIQIEVITGDSEGKAIMQLPLEQIGLNPALHYLYIDVGGGSTELAFIGKGDLTANRS
                     FNIGTLRLLNDQVSDADWKEIPDWIKAHKHHGFDLHAIGVGGNINHVMKHYGKAGKPY
                     ISMAILKRVMMYIESLSMGERVRTLGLKPDRADVIVPALKIYINVMKWAGIEKIYVPK
                     QGLSDGLISQLYHEWKSKVVTP"
     gene            complement(1478..2029)
                     /locus_tag="PLL28_13810"
     CDS             complement(1478..2029)
                     /locus_tag="PLL28_13810"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF70452.1"
                     /translation="MTRSDNYVSPTIFTDRLILRNPSLNDAFNMFLQRTDAEVNQFVP
                     MTPPKDPSEVETLLRTIIHNTSTGISDYWIITDKQNTYLGSICLWNYNYESNTAELGF
                     VLIKNAWNHGYATEAIERVITFAMEKLPFTIIEGWTHHQNFAAQHAMQKNGFLRNMEA
                     EKNIDQSSPDADMQIWTLKLIRE"
     gene            complement(2026..2628)
                     /locus_tag="PLL28_13815"
     CDS             complement(2026..2628)
                     /locus_tag="PLL28_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNF70453.1"
                     /translation="MPTLYKNIKICIGKSPTAWRNWLEKNHAKEEKVWLVMFKKNSGI
                     PSLTWEEAVDEAICFGWIDSVRNSRDETSFYQYFAKRKPKSGWSDINKKKVERLLNEG
                     KIAPAGLEMIQLAKSTGTWDTLNEVETLAIPPDLQSALKKNKTALKHFNAFPASTKRA
                     ILQWLQSAKKEETRKRRIAQTVAEASQNIRTTQTNQPKLK"
     gene            complement(2979..>4101)
                     /locus_tag="PLL28_13820"
     CDS             complement(2979..>4101)
                     /locus_tag="PLL28_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70454.1"
                     /translation="LNDLDATLPHEFSIDIVDNEIIEGFHEGNITFEIITDDPVLTGV
                     TIDPLVISIDDNDFPQSVIISVDGFTHTEGEAGIPFSVALESVPDLDADILLSPSEDI
                     DLGAGFGAIVNLHFENDETAMTPIILTIAVKDNFIVDGDHIGSILAELITDDPFLSDY
                     LIPDITINILDNDAEQGVNITTPGTTFYAEGTTGLSVSLSLFTIPNDIVTISATYDDT
                     QIDLGLIESLTFATEATAMEMQYFNVDIIDDAIVEGDHYALINYIITTDDPQFDGYTI
                     PFTMINISDNDVESGISNATTDNLPTVVVTSGQFQINMPDSYNGKDFAITSINGQIVS
                     KGIIDGNLLHGDLSRMSAGRYMIAIVDGEKMLSIPVILN"
BASE COUNT         1168 a          698 c          946 g         1289 t
ORIGIN      
        1 attggtagca ccaattaatt taaggtcgcg attgaagttg ctcattaaca gagaaattaa
       61 attcgccaaa gagaaaaaaa aggcagcaat ttttttgaaa gtgaataacc tggtcgataa
      121 ggagatcata aagaaattat atgaggctag tatggctggc gttcagatta aattaatcgt
      181 gcgtggcgct tgttcaattg ttccgggtgt tgaggggttg agtgataata tcgaagccat
      241 cagtgtatta gataggtttt tagagcatcc gagggtgttt gcatttcaca ataatggtaa
      301 agaggaagtg ttcatcggat cggcagatat tatggagcgc aacttagatc atcgtataga
      361 agtaatggta caggtattgg ataaggatat cagaaagatg ataaaagaca tattgaccat
      421 acaatggggc gacaatgaaa aggcgcgttt gctggggcat gggcatttga atgattatgt
      481 caaagtgttg ccaggagaaa agcaagtaag gagtcagatg gcgctatatg attattttga
      541 aaaatcgcgc ctggcatcgg cgttagaaaa aaaggaaggt aataagtaaa tatgaaattt
      601 gcagcgatag atattggaag taatgcagtc agattattga ttgaaaatgt atatgagaca
      661 tcttcaggac cggtatttca taaagacagt ttgatcagaa tacccgtccg attgggagag
      721 gacgcatttt tggatggcgc tatttctgag gagaaagtgc agatgcttgt caaagcgatg
      781 gttgccatgc gacatatcat tgacatccac aaagtgactg ctataaaagt tgcagccaca
      841 tcagcgatgc gtgaagctgc taatagtgga gatatcatta atcgggttat ggaagcttcc
      901 gggattcaaa ttgaagtgat aacaggcgat agtgaaggca aagccattat gcaattgcca
      961 ttagaacaaa ttggattaaa tccggcatta cattatttat atattgatgt tggtggaggt
     1021 agtactgaat tagcatttat tggcaaagga gatttaacgg cgaacaggtc gtttaacatt
     1081 ggcacgttac ggttgttaaa tgatcaagtt agtgatgcag actggaagga gatacctgat
     1141 tggataaaag cccataagca tcatggattt gatttgcatg caataggtgt tggaggcaat
     1201 attaaccatg taatgaagca ttacggtaaa gcaggcaagc catatatttc gatggcaata
     1261 ttgaagcgcg tgatgatgta tattgaaagt ttgagtatgg gtgaacgggt acgaacattg
     1321 ggattaaaac ctgatcgcgc tgatgtgatc gttccggctt tgaaaatcta tatcaacgtg
     1381 atgaaatggg cgggaattga aaaaatatat gttcccaagc agggattgag cgatgggctt
     1441 atcagtcagt tgtatcacga gtggaaatca aaggtggtca ctccctgatt agtttaagcg
     1501 tccatatttg catatcggca tcagggcttg attgatcgat atttttttca gcctccatat
     1561 ttctcaggaa tccatttttt tgcatggcat gttgagcagc aaaattttgg tgatgtgtcc
     1621 agccttcaat tatagtgaat ggaagttttt ccattgcaaa tgtgatgaca cgttcaatag
     1681 cttctgtggc ataaccatga ttccatgcat ttttgatgag cacgaaacct agttctgcgg
     1741 tatttgattc gtaattataa ttccagaggc aaatactgcc caggtaggtg ttttgtttat
     1801 cggtaattat ccaataatcg gaaattccgg tgctggtgtt atggatgatg gttcgcaaaa
     1861 gagtttccac ttcggaggga tcctttggtg gcgtcatcgg gacgaattga ttcacttcgg
     1921 catcagttcg ttgcaggaac atattgaatg catcatttaa tgatggattg cgaagtatta
     1981 gtcgatccgt aaatattgtt ggagaaacgt aattgtcaga cctcgtcatt ttaattttgg
     2041 ttgatttgtt tgtgttgtcc taatattttg gcttgcttca gctacagttt gggctatacg
     2101 tcgttttctg gtttcttctt ttttggcgct ttgcagccat tgaaggatag ctcgttttgt
     2161 tgaagcagga aaggcattaa aatgtttaag agctgttttg ttttttttca gcgccgattg
     2221 aaggtcggga ggaattgcca gtgtttcgac ttcattgagt gtatcccagg tcccggtgct
     2281 ttttgcaagt tggatcattt cgagtcctgc aggcgctatt ttcccttcgt tgagtaatcg
     2341 ctctactttt ttcttattga tgtcactcca accacttttt ggcttgcgtt ttgcgaaata
     2401 ttggtagaag ctggtttcgt ctctgctgtt tcgcacggaa tctatccaac cgaagcagat
     2461 agcttcatca acggcttctt cccaggtaag agaagggatg ccgctatttt ttttgaacat
     2521 cacaagccag accttttctt ccttagcatg atttttttcg agccagttgc gccatgcggt
     2581 tggagatttg ccgatgcaga ttttaatgtt tttgtagagt gttggcattg tgaggtgaaa
     2641 gtagggtagg gagttggatt gtcaaaattg gggggcaaac ctttcgtgtt ttaaaccttc
     2701 catgtttcaa acctaacagg tcttaaacct gtcacatttt agacctaaaa ggtttaaaac
     2761 gcgacaggtt taagacctgt ttacgtgaac cgcttacatc gcggtgctta ctcacctctc
     2821 gccaaattga gttttttagg aaattttcaa atcagagcaa tttgtcatgt cacaaatctc
     2881 ctttttttag caagttgctt taagcgagta taccattatt aaacctgcct tcgcaagtta
     2941 tttacttgaa ccattgagaa attttatatt tcttcagatc aattcaaaat cacgggaata
     3001 gacaacattt tttcaccatc tacaatcgca atcatatatc tgccagccga cattctactt
     3061 aggtcgccgt gtaacaaatt gccatctatg atgcctttag acacaatctg accatttatc
     3121 gaggtgatcg caaaatcttt gccgttataa ctgtcaggca tattgatttg gaattgtcct
     3181 gaggttacaa ccacagttgg taagttgtcg gttgttgcat ttgaaatgcc ggattcaaca
     3241 tcattgtctg aaatattaat catggtaaat ggaatagtgt atccatcaaa ctgaggatcg
     3301 tcggtagtga tgatataatt aatcagagcg taatgatcgc cttctacaat agcatcatca
     3361 atgatgtcga cattgaaata ttgcatttcc atagcagtgg cctctgtcgc gaaggtgagt
     3421 gattcaatta atccgagatc aatttgcgta tcgtcatagg ttgccgagat tgttactatg
     3481 tcattaggta tggtgaatag agaaagtgaa actgataaac cggttgtgcc ttcggcatag
     3541 aatgttgttc ccggagtggt gatgttgaca ccttgttctg catcattatc taaaatattg
     3601 atagtaatgt caggtatcag gtagtctgac aagaatgggt catcggtgat cagttctgca
     3661 agtatgctgc cgatatgatc gccatctacg atgaaattat ctttcacggc aattgtcaag
     3721 ataattggtg tcattgcagt ttcatcgttt tcaaaatgca gattcacaat agcgccgaag
     3781 cctgcaccaa gatcgatatc ttcggatggt gacaacaaaa tgtcggcatc aagatcagga
     3841 acagattcta aagccacgct gaatggtatt cctgcttcac cttcagtatg tgtaaatcca
     3901 tctacgctga ttatcaccga ttgtggaaaa tcattgtcat ctattgaaat taccagcgga
     3961 tcaattgtca ctcctgtcaa cacaggatcg tctgtaatga tttcgaatgt gatatttcct
     4021 tcatggaagc cttctattat ttcattatca acaatatcaa tagaaaattc gtgaggaagt
     4081 gtagcgtcca aatcattcaa t
//
LOCUS       DAOQHY010000243         4070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_13313_length_4070_cov_5.175841, whole genome shotgun sequence.
ACCESSION   DAOQHY010000243 DAOQHY010000000
VERSION     DAOQHY010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13313_length_4070_cov_5.175841"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1473
                     /locus_tag="PLL28_13825"
     CDS             <1..1473
                     /locus_tag="PLL28_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF70455.1"
                     /translation="PMTSLNPVFTCGAQVDEAIKLHQKKNKHEARALTISLFEKVQLP
                     NPERIYRAYPHELSGGQKQRVMIAMAMSCNPDILIADEPTTALDVTVQKTILQLMEEL
                     KSTIHSSIVFITHDLGVIAEIADRVLVMYKGRIVEEGTVLEIFESPKHPYTKGLMACR
                     PPLGKRLRKLPVIADFMEESADGELIEKQQSVEQAVKINEVNSTETKERLSKLIVQEP
                     VLKVRNLKTWFPSKKSFLGKVEGWIKAVDDVSFDVYPGETLGLVGESGCGKTTLGRTI
                     IRLVDPISGEVHFDNQNLMTLTHRELKPIRKDIQIIFQDPYSSLNPRMTVGNAVREPM
                     QVHGMYSSDRERKEKVIELLEKTGLKADHYNRYPHEFSGGQRQRICIARALALNPKFI
                     ICDESVSALDVSVQAQVLNLLIQLRNEFNFSYIFISHDLSVVKFMSDRMVVMNKGVIE
                     EMGPADEIYDNPQKAYTQKLIDAIPKGQLSDIKGRLMQLQ"
     gene            1498..3582
                     /locus_tag="PLL28_13830"
     CDS             1498..3582
                     /locus_tag="PLL28_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70456.1"
                     /translation="MTRRPIGFLLVIMALFTIVSCRKDLNTGWDTRYKLPIVTGELDL
                     YDLVPDSLTVIGDDQSVTLVYKTELLDYDLTEEAIEIPDTSVEYFVSLDSLSLEDQDL
                     TIPISLGQIAIDLGFPYGTLIIAANGTEVALDPIYGLSTDPTPVDATTFFETATFQHA
                     YLDISLANGLPVDLDTVVFSLKNSVGGETVVYDTFFTVPAGGVPVVETYDLTGMTVEG
                     NLLGQIAYFSTPGSADPVLIDTSDVLVIDMRAYDMELQEAVAVFPEQNLINNANDVQY
                     DMGGPEFTTMGIEEGYVVIYVENTIGDTIHIVYDIPGAVDALGNHVHIETAVGPDEIV
                     DQAFDIAGYTIDLTGTSGNEVNTFYQEFSARINYTGIPTHISLDDSLRVIYGLENIVP
                     NELRGYLGQYEIAMADTTAGVDLFDQFTGGTIEFGDINIDLEIENGLGAGGVVTINSL
                     GGINSETGEVIMLDCPAVIGTPISVNRAIDNPYVPGYTTITLNSANSNINDLIEMFPD
                     KLFYDVSMSVNPNGNEFNYQDFVISTSKINISFNLSMPLEFMANDLTLESEFPVTLDA
                     DGGTDGIGDIDLTLYADNSFPLNASVSIVFYDDFGVALDTLNFGGYNIKAGELSDDCR
                     VHTPMGTELHQLVDGTMKDAVLNAANAKATVTFNTESIPTCSDIVKIYSDYTMKIQLV
                     GDINYTLSTSDF"
     gene            3586..>4070
                     /locus_tag="PLL28_13835"
     CDS             3586..>4070
                     /locus_tag="PLL28_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70457.1"
                     /translation="MQLTKILICFALAIVCTTAQANPDSTATKSSAEYTYTAPTTIFG
                     IYDPLNVSLRDNRFAFTAYGDLFLASNCFSASFLQNFFGSGFITEEMKDFASEKLSAE
                     NTLGVDLSAGLWMQMAMKNNADNIFIAGIDYNFEESSQFTPDLFHLAFYGNYDLQGET
                     A"
BASE COUNT         1202 a          838 c          867 g         1163 t
ORIGIN      
        1 ccgatgacga gtttaaatcc tgtttttact tgtggcgcac aggtggatga agcgataaaa
       61 ttgcatcaga aaaaaaataa acatgaagca cgcgcactta ccatttcatt attcgaaaaa
      121 gtgcaattgc caaatcctga aagaatatat cgcgcttacc cacatgaatt aagtggtggt
      181 caaaaacaac gtgtcatgat tgccatggcg atgagttgca atccggatat attaattgcc
      241 gatgaaccaa caaccgcact cgatgtaact gttcaaaaaa cgatattgca gttaatggaa
      301 gaattaaaat ctaccattca ttcgtctatc gttttcatca cccacgatct tggtgtgatt
      361 gcagaaattg ccgacagggt attggtgatg tataaaggtc gcattgttga agaaggtacg
      421 gtgctcgaga tctttgaaag ccccaaacat ccctacacca aagggcttat ggcctgtcga
      481 ccgccattgg gaaagcgtct tcgcaagctg ccggtgattg cagatttcat ggaagaaagt
      541 gccgatggtg aactcataga aaaacaacaa tcagtagaac aggccgtaaa aattaatgaa
      601 gtaaacagca ctgaaacaaa agagcgatta tcaaaattaa tagtacagga accggttcta
      661 aaagtgcgca acctgaaaac ctggttccca tcgaaaaaaa gtttcctggg aaaagttgaa
      721 ggatggataa aagcagttga tgatgtatca ttcgatgttt atccgggaga aacacttgga
      781 cttgttggtg aaagcggttg cggaaaaact accctcggaa gaacaattat cagattggta
      841 gatccgattt caggtgaggt gcatttcgat aatcagaatt taatgacact cacgcatcgc
      901 gagttaaaac ccatcagaaa agatattcaa attatttttc aggatccata ttccagttta
      961 aatccgcgca tgacagttgg caatgcagtt cgtgagccaa tgcaagtgca cggcatgtat
     1021 tcgagcgatc gtgagcgaaa agaaaaagtg atagaacttc tggaaaaaac aggtttgaaa
     1081 gcagatcatt ataatcgcta tccgcacgaa ttttccggag gtcagcgcca gcgtatttgc
     1141 attgccagag ccttggcatt aaatccgaaa ttcattattt gcgatgaaag tgtttcagcc
     1201 ctcgatgtga gcgtgcaggc gcaagtactc aacctgctca ttcaattgcg caatgaattt
     1261 aacttctcct atatcttcat ctcccacgac ctcagtgtag taaaattcat gagcgaccgc
     1321 atggtggtaa tgaataaggg cgttatcgaa gaaatgggac cggcagacga aatttacgat
     1381 aacccgcaaa aggcttacac ccaaaagctg atcgacgcca ttccgaaggg tcagcttagt
     1441 gacataaagg ggcgcctaat gcaactgcaa taaagattta cgtatcttca caacatcatg
     1501 acccgcagac ccattggctt cctcctcgtg ataatggcac tgtttacaat tgtgtcgtgc
     1561 cgaaaagacc tcaataccgg ctgggataca cgttataaac tacctattgt taccggcgaa
     1621 ctggatctct acgatcttgt tcccgactca cttaccgtaa tcggcgatga tcagagcgtt
     1681 accctggttt acaaaacaga attgctcgat tacgacctga cggaagaggc tattgaaatt
     1741 cctgatacca gtgtggaata ttttgtttca ctcgattcat tgtcgcttga agatcaagac
     1801 ctgactatcc ctatctccct tggtcaaatt gcaatagacc ttggctttcc ttatggaacg
     1861 cttatcattg ccgccaatgg aacagaagtt gccctcgatc ctatttacgg tctgtcaaca
     1921 gaccctaccc ctgttgatgc caccacgttt tttgaaactg cgactttcca acatgcttat
     1981 ctggatattt ctttggcgaa tgggttaccc gtcgatttag atactgttgt tttttctttg
     2041 aaaaattcag ttggtggtga aacagtagtt tacgatacct tttttaccgt gcctgccgga
     2101 ggcgtaccag ttgttgaaac atacgatctt accggaatga cggttgaagg taacctcctc
     2161 ggacaaattg cttatttcag cacaccgggt agtgccgatc ctgtgctcat cgataccagc
     2221 gatgttttgg tgatagatat gcgtgcttac gacatggaat tgcaagaggc cgttgctgtt
     2281 ttccctgagc aaaatctgat taacaatgcc aacgatgtgc aatacgatat gggtggcccc
     2341 gaattcacta ccatgggtat tgaagaagga tatgttgtca tttatgtaga aaataccatt
     2401 ggtgatacca ttcacatcgt ttatgatatt ccgggggccg tagatgcatt gggaaatcac
     2461 gtgcatattg aaacggctgt cggccctgat gaaatcgtag atcaggcttt cgatattgca
     2521 ggttatacca ttgatctgac aggtaccagt ggaaatgaag taaatacatt ttatcaggaa
     2581 tttagtgcac ggataaatta cacaggtatt ccaacacata tttccttaga cgatagtttg
     2641 cgtgtgattt atggacttga aaatattgtt ccgaatgaat tgcgcggtta ccttggacaa
     2701 tatgaaattg ccatggcgga tactactgca ggtgttgatc ttttcgatca atttaccggt
     2761 ggcacaattg aattcggtga tatcaatatc gatcttgaaa ttgaaaacgg acttggagca
     2821 ggtggtgttg ttaccattaa ttctctcggt ggtatcaatt cagaaacagg tgaagtgatc
     2881 atgctcgatt gtcctgctgt aatcggcaca ccaatttccg taaatcgcgc tattgataac
     2941 ccttatgttc ccggttatac taccatcaca cttaattcag ccaattccaa catcaacgac
     3001 cttatcgaga tgttccctga taaattattc tatgacgttt ccatgagtgt aaatccaaat
     3061 ggtaatgaat ttaattatca ggatttcgta ataagcacca gcaaaataaa tatctcattt
     3121 aatctgagta tgccgcttga gtttatggcg aatgatctta ctctcgaaag tgaattccct
     3181 gttacgcttg acgcagatgg cggcacagat ggcattggcg atatcgatct tactttgtat
     3241 gcagataatt ctttcccatt aaatgcatca gtgagcatcg tattttatga tgattttggt
     3301 gttgcactag atactttgaa ttttggcgga tataatatta aagccggtga attgagcgac
     3361 gattgccgtg tgcatacacc aatgggcacc gaattacatc aacttgtaga tggcaccatg
     3421 aaggatgccg ttttaaatgc tgccaatgca aaggcaaccg taacatttaa taccgagtcc
     3481 attcctacat gcagcgatat cgttaagata tacagcgact ataccatgaa gatccaattg
     3541 gtgggcgaca ttaattacac actctcaact tcagatttct aaaacgtgca actgacgaag
     3601 atattaattt gctttgccct ggcgatagtt tgtacaactg cgcaggcaaa ccccgattct
     3661 accgctacca aatcatctgc tgagtatact tatacagcgc caaccaccat ttttggtatt
     3721 tacgatcctc tgaatgtttc actgagagat aatcgtttcg cttttactgc ttatggcgat
     3781 ttattcctgg caagtaattg tttcagtgcc agcttcttac agaatttctt tggaagtggt
     3841 tttatcactg aagagatgaa agattttgca tctgaaaaat tatctgccga aaacaccttg
     3901 ggtgtagacc ttagtgcagg gttatggatg caaatggcaa tgaaaaataa tgccgataat
     3961 atttttattg ccggcatcga ttataatttc gaagaaagca gtcaattcac ccccgatctt
     4021 tttcatcttg cattttatgg caattatgac ctgcagggag aaacagcaat
//
LOCUS       DAOQHY010000244         4038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_13504_length_4038_cov_6.154908, whole genome shotgun sequence.
ACCESSION   DAOQHY010000244 DAOQHY010000000
VERSION     DAOQHY010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13504_length_4038_cov_6.154908"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="PLL28_13840"
     CDS             <1..862
                     /locus_tag="PLL28_13840"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNF70458.1"
                     /translation="QHGTAAMKLIIVNVAIYLAQSLGLLVAYGFHQKEAYLDFLVNWF
                     WANSNISTLATRPWTLVSYMFMHDPQNIFHIVSNMIYLFFFGRIFSDITNQKLAYPLY
                     FAGGFAGFFLALISFYLFPGYQENIGGYFVGASAAIMAIVIATTTLMPNYTVFLYFLG
                     PVKLKYIAAFVVIVDVISIPGESNIGGHIAHLGGALFGYLYIRSYRKSGNWFSWWPHF
                     ESRVWQIFHRNKLRVAYNYQRQDTDDRRAKEIEDQKKLDAILDKIKASGYESLSRQEK
                     DFLFRMSKEK"
     gene            864..1967
                     /locus_tag="PLL28_13845"
     CDS             864..1967
                     /locus_tag="PLL28_13845"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNF70459.1"
                     /translation="MRWWQKLILFITIIFALLLIGAMLAMYISPEKFWPLAFLGLGYF
                     PILLCYMFFMLLWLFMRRKIFYGMLIILTLGFTAHLRHFSLGGLKRSSDSEKPVYTLM
                     QYNVQGFDAYNKEGKYKNRDQIIANIANAKPDILCMEEFNTYTNHRKEKSNLDMVMTA
                     TGLKNYYYYKAFENPKTTRSFGLMIMTKFPIIDTGHLEYLSLSPLNTTIYADLLIEND
                     TVRVFCSHMQSTQLNHYDLEFIEASNEAETDFDADRITTKLKMSFSLRAQEADTVATH
                     IKASPFPMISCGDFNDTPVSYTYHKMSHGMEDAFLKRGFGVGATYAPFPFIRIDFMLF
                     KDDAFDIHDYYTIKDKSSDHYPCITKFSIPAKD"
     gene            complement(2108..>4038)
                     /locus_tag="PLL28_13850"
     CDS             complement(2108..>4038)
                     /locus_tag="PLL28_13850"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HNF70460.1"
                     /translation="TSAETFTVNFTVKEKVSGTVRYSSSSGVSFTAGEVKTVTATGWN
                     PTIQETDTVIVDVPALPGETVVANNSLGYRHVVNSDTYGYADLSAATGGVGFGAGAGQ
                     ILNRHTLTGCSSVEAIRVYIFGAASIGNTIRGVVNDITGIQIGVSADHVVTAGEVDTW
                     ITLPILSPPFITNQDVLFGIEQTAGIGAAAYFPVGTQTESSPTRDFAFYSNNPGGGAL
                     VGPYTTLNRWMIEGVVTPLSTDPTVSGPTEACDGDPISFTASFTELNPAAVVNWYTDG
                     CGTTLVGTGMTFSTTAMAGMTTYYVRAEDACNGVNTACASITITVGVSEVYFADADGD
                     TYGDPGATVLSCDGAPTGYVLDPSDCDDTNSSVYPGATEVCDGLDNNCDGNIDEGVVE
                     AVITAPGGLVACKPLSVLLQTTAIPGYTYQWFKNGNIITGAINPTYSASKPAYYQVQV
                     NTPGGCFALSDAVLVNILNAPNANISAPNGTSLCTTVKLKASYDATYTWQWRLGGVDI
                     GGATNYLYYPSTAGSYTCVVTSAEGCSRETASIAVTACREGGAISEEINTINVYPNPS
                     AGTFTLEIMSGDEFSGKADVYVMNMIGEVIYNNNIDITNGYINNTITLADGIAGGLYL
                     VKVMANGHEYTQQVVISK"
BASE COUNT         1192 a          835 c          816 g         1195 t
ORIGIN      
        1 gcaacatggc actgcagcca tgaagctgat tattgtcaac gtggctattt acctggctca
       61 aagcctaggc ctgcttgttg cttatggatt tcatcaaaaa gaagcctatc tggattttct
      121 cgtaaactgg ttctgggcga attcgaatat cagtacactc gcaactcgcc catggacttt
      181 ggtcagctac atgttcatgc atgatcctca gaatatattt catattgttt ccaatatgat
      241 ctatttattt ttctttggac gcattttcag tgacatcacc aatcaaaagc ttgcttatcc
      301 gttatatttt gccggaggtt tcgccggatt ttttcttgca ttaatttcct tttatctttt
      361 tccgggctat caggaaaata ttggggggta ttttgtaggt gcctcagcgg ctatcatggc
      421 aattgtaatt gctaccacca ccttaatgcc aaattacacc gtgtttttgt actttttagg
      481 ccctgtgaaa ttgaaatata tcgcggcatt cgttgtgata gttgatgtga taagtattcc
      541 gggagaaagc aacattggtg gacatattgc ccatttaggc ggagcattgt ttggctattt
      601 gtatattcgc agttatagaa agtcgggcaa ttggttcagt tggtggccgc attttgaatc
      661 tcgggtatgg caaatatttc atcgcaataa gttgcgcgtt gcctataatt atcagcgcca
      721 ggataccgat gatcgcagag ctaaagaaat tgaagatcaa aaaaaacttg acgccatact
      781 cgataaaata aaagccagtg ggtatgaaag tttaagcagg caagaaaaag atttcctatt
      841 cagaatgagc aaagaaaagt aatatgcgct ggtggcagaa attgatatta ttcataacca
      901 taattttcgc cttgttacta attggcgcaa tgctggctat gtatatcagt ccggagaaat
      961 tttggccttt agcttttctt gggctgggat attttcccat tttactctgc tatatgtttt
     1021 tcatgttgtt gtggcttttt atgcggagga agatttttta tggcatgttg atcattttaa
     1081 ctttaggttt tacagcccat cttcgccact ttagtcttgg tggtttaaag cgatcttccg
     1141 acagtgaaaa accggtttac accttaatgc aatataatgt gcaaggattc gatgcttaca
     1201 acaaggaagg taagtataag aaccgcgatc agatcattgc caatattgca aatgccaagc
     1261 cggatatatt gtgtatggaa gaattcaaca cttacaccaa tcacagaaag gaaaagagta
     1321 atcttgacat ggtgatgacg gcaaccgggt taaaaaatta ctattactat aaagcatttg
     1381 aaaatccgaa aacgaccaga agtttcggcc ttatgattat gactaaattt ccaataattg
     1441 atacaggtca tttagaatat ctttcattaa gtccgctgaa tacaaccatt tacgccgatc
     1501 tgttgattga aaatgatacc gtacgcgttt tttgcagtca catgcaaagc acgcaactca
     1561 atcattatga tctggaattc attgaagcca gcaatgaagc ggagactgat tttgatgccg
     1621 acaggatcac aacaaaatta aaaatgagtt tttctttgcg tgcacaggaa gctgacactg
     1681 tagccacaca tataaaagca tcaccatttc cgatgatttc gtgtggcgat tttaatgaca
     1741 cccctgtaag ttacacatat cataaaatgt cgcacggaat ggaagacgct tttttaaaac
     1801 gcggttttgg tgttggagca acatatgcgc cgttcccgtt tatacgcatt gattttatgt
     1861 tatttaaaga tgatgctttt gatattcacg attactacac tataaaagat aaatcgagtg
     1921 atcattaccc ttgcattact aagttcagca tccccgctaa agattaactt atttcaagat
     1981 cgctttcaag gataataccg gcttcaatta atctttaatt ccttgcatat aggcatagaa
     2041 gaaatgcaac aaataaaaaa gacccggagt cgcttccggg tcttttctgt ttcgcagaac
     2101 aatttgatta tttggatata accacttgct gcgtgtactc gtggccattg gccattacct
     2161 tcaccaaata aaggccacca gctataccgt cagccagagt aatggtatta ttaatatagc
     2221 cgttagttat atcgatattg ttgttatata taacttcacc gatcatattc atcacataca
     2281 catcagcttt tccggagaat tcgtcacctg acataatttc aagtgtgaat gttcctgctg
     2341 aaggatttgg ataaacatta atagtattaa tttcttcgct tattgcacca ccttcgcggc
     2401 aagcagtaac agcgattgaa gcagtttcac gtgagcaacc ttcagcgctt gtaacaacgc
     2461 atgtgtagct tcctgcagtt gatggataat acaggtagtt tgttgctcca cctatgtcaa
     2521 caccacctaa gcgccattgc caagtatagg tagcatcgta gctggctttc aacttaacag
     2581 tagtacaaag acttgtgccg tttggtgcgc tgatgttagc attaggtgca ttaagaatat
     2641 tcacgagcac agcatcagac aatgcaaaac aaccacccgg tgtgtttact tgtacttgat
     2701 aatatgcagg tttacttgca gaatatgttg ggttaatagc tcctgttatg atattaccat
     2761 ttttaaacca ctgataagta tatcccggaa tagcagttgt ttgcaataaa accgacaatg
     2821 gtttacatgc taccagtcca cccggagcgg tgatcactgc ttcaacaaca ccttcatcga
     2881 tattgccatc gcagttatta tcaagaccat cgcaaacttc tgtagcaccc ggataaacag
     2941 atgagttagt atcatcacaa tcagaaggat caagtacata tccggtaggc gcaccatcgc
     3001 aacttaatac tgtagcaccc gggtcaccat aagtatcacc atcggcatca gcaaaataaa
     3061 cttcagaaac accaacagtt atagtgatag aagcacaagc agtatttaca ccgttacatg
     3121 catcttcagc acgaacgtag taagttgtca ttcctgccat tgcagtggta gagaatgtca
     3181 tgccggttcc aacgagcgtt gttccgcaac catctgtgta ccagttaaca acagcggcag
     3241 ggttgagttc agtaaatgaa gcagtgaaac ttataggatc accatcgcag gcttctgtag
     3301 gacctgaaac agtaggatct gtacttaacg gcgtcacaac accttcaatc atccagcggt
     3361 taagcgtagt ataaggacca actaacgcac caccaccggg gttatttgaa taaaatgcaa
     3421 aatcgcgtgt tgggctggat tctgtttgtg taccaacagg gaaataggca gcagcgccaa
     3481 tgcctgcagt ttgttcaata ccaaacagca catcctggtt agtaataaac ggtggcgaca
     3541 gaataggaag cgtaatccat gtgtcgactt caccggcggt tactacgtga tcggcagaaa
     3601 cgccaatttg aataccggtg atatcattta cgacaccgcg aatggtattg cctatactcg
     3661 ctgcaccaaa aatatatact ctgattgctt caacactgct gcatcctgtt aatgtatgtc
     3721 ggtttaatat ttgtcctgca cctgcaccaa aaccaactcc accggttgca gcacttaaat
     3781 cggcataacc ataggtatct gaattaacta cgtgacgata accaagtgag ttgttagcaa
     3841 caactgtttc acccggcaaa gcaggaacat caacaatcac agtatctgtt tcctgaatgg
     3901 ttggattcca acctgttgcg gtaacagttt tcacttctcc tgcagtaaat gaaacacctg
     3961 aagagctgga ataacgaact gttccggata ctttttcttt tacggtaaag tttacggtga
     4021 aagtttcggc ggaagttg
//
LOCUS       DAOQHY010000245         4025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_13576_length_4025_cov_4.412091, whole genome shotgun sequence.
ACCESSION   DAOQHY010000245 DAOQHY010000000
VERSION     DAOQHY010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13576_length_4025_cov_4.412091"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PLL28_13855"
     CDS             complement(<1..382)
                     /locus_tag="PLL28_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNF70461.1"
                     /translation="MAQLQLDKPLAFIDLETTGINLGSDRIVEISILKLFPNGNRETK
                     TMRINPGIPIPAESSAVHGIYDADVADKPTFAQAADELNAYLDNCDIAGYNSNKFDVP
                     MLVEEFLRCGKGFDENRRYIDVMRI"
     gene            complement(391..1731)
                     /locus_tag="PLL28_13860"
     CDS             complement(391..1731)
                     /locus_tag="PLL28_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNF70462.1"
                     /translation="MRIHLIAIGGAVMHNMALALHAVGHQVSGSDDEIFEPSKSRLSR
                     KGLLPEAFGWFPERITSELDAVILGMHARADNPELLRAQELGIRVYNFPEYVYEQSKN
                     SLRVAICGSHGKTSITSMIMHVLKKRQMPFDFLVGAQLEGFDTMVQFSQAPVTIIEGD
                     EYFASPLDKRPKFLFYHAQVKVISGIAWDHFNVFPTFDNYVDQFRQLVNQSTENDILI
                     ANADDDEVITLLDISRPKGKIIKYGAPAYKVIEGSFTLDVDGAHYQFAIFGRHNMQNM
                     EAAKNVCISLGISAHEFYEAMQSFTGAAKRLEVLKRSSDHIIFRDFAHAPSKVKATVD
                     AVREQFPDQPLVACLELHTFSSLNKDFIAHYHGTMQAADKRIVYFNPHTLTLKKLPML
                     SIEEVRSFFGDKELIVLNDSSSLKSELQSLNSVNNNILLMSSGNYDNLDLTFLT"
     gene            1896..2036
                     /locus_tag="PLL28_13865"
     CDS             1896..2036
                     /locus_tag="PLL28_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70463.1"
                     /translation="MRTFSKWAVAAGLCLLLTAGLSSCGHKEGCPNKITEVGGIKIET
                     NA"
     gene            complement(2091..2495)
                     /locus_tag="PLL28_13870"
     CDS             complement(2091..2495)
                     /locus_tag="PLL28_13870"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNF70464.1"
                     /translation="MITSQVTYEGELRTTAVHTLSGTSIITDAPPDNQGKGEAFSPTD
                     LVATALASCMLTIMGIAARTHEMDIQGTRVEVTKIMASNPRRISEVHCRLFIPGNFND
                     KQKAILEHAARTCPVIQSLSSDMIKVVTFSYA"
     gene            2554..3360
                     /locus_tag="PLL28_13875"
     CDS             2554..3360
                     /locus_tag="PLL28_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70465.1"
                     /translation="MKFVFFCMAWMAVVYSAVAQDTSTAKQNTSKPVILNKSSASKPK
                     GLSYEHEFSMGAKVTTTGWGIFGDYTKDIDLDKKRVAYFELEFLKDPKETKRVNEFTV
                     GYTPPKPFVYGKQNTFFNLKAAIGEKRMIGEKAEKSGFQVAANYAGGFSAGLVKPYYL
                     AVYSTSEDGTPTENVKYTPETESLFLDYTSIYGASGFSIGFNEISFVPGLFGKAGLNF
                     DWATYDDFVKSAEAGIGAELYLKDIPIMILEDNKPYFVYLYLSLQFGKKW"
     gene            3379..>4025
                     /locus_tag="PLL28_13880"
     CDS             3379..>4025
                     /locus_tag="PLL28_13880"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNF70466.1"
                     /translation="MIELPVTESQERQRKPKWLKVKLPTGENYRMVRELVDQHKLHTI
                     CESGNCPNMGECWGEGTATFMILGNVCTRSCSFCAVATGRPTELDLEEPFRVAEAIKL
                     MGVKHAVITSVNRDELADRGAAIWNRTVTEVKRLSPQTTIETLIPDVKSNWDALEMMI
                     SPGQEVVSHNMETVKRLYRLVRPQAKYERSLEQIKRTKDYGKRTKSGVMLGLGESA"
BASE COUNT         1166 a          892 c          866 g         1101 t
ORIGIN      
        1 aaatgcgcat cacatcaata taccgacggt tctcatcaaa accctttccg caacgcagga
       61 actcctccac caacatgggt acgtcaaatt tgttgctgtt gtagcctgca atatcacaat
      121 tatccaaata agcattcaac tcatccgcag cctgagcaaa tgtgggtttg tcggcaacat
      181 cagcatcata gataccatga acggcactgg actccgcagg aatcggaata ccgggattaa
      241 tacgcatagt cttggtttcg cgattgccgt taggaaataa ttttaagatg ctgatctcaa
      301 ctatgcgatc gcttccgaga ttgataccgg tggtctccag atcaataaag gcaaggggtt
      361 tgtccagttg cagttgtgcc acgctcaaaa ttaggttaaa aatgtaagat cgaggttgtc
      421 gtaattgccc gacgacatta acagaatatt attattcaca ctatttaacg attgtaattc
      481 actttttaat gacgaggaat cattcaatac aatcaattct ttatccccaa aaaaacttct
      541 gacctcttca atactcaaca tcggcagttt ttttaaggta agagtatgcg gattgaaata
      601 cacaattcgt ttgtcggcag cctgcatggt tccatgataa tgtgcgatga aatctttatt
      661 caaactcgaa aaggtatgta attccagaca tgccactaaa ggctgatcgg ggaattgctc
      721 gcgtaccgca tcaacagtag cttttacctt gcttggtgca tgcgcaaaat ctctgaaaat
      781 aatatgatcg ctgctgcgct ttaatacttc taatcgcttc gctgcacctg taaacgattg
      841 catggcttca taaaattcat gtgcagaaat gcctagcgat atacatacat ttttcgccgc
      901 ttccatattc tgcatattgt gccttccgaa tatggcaaat tgataatgtg cgccatccac
      961 atcaagcgta aatgaacctt caattacttt atatgcaggg gcgccatatt tgatgatctt
     1021 gccttttggg cgactgatat ccagtaatgt tattacctca tcgtcgtctg cattggcaat
     1081 taaaatatca ttttcggttg attgatttac taattgtcgg aattgatcaa catagttatc
     1141 gaaagtggga aatacattga aatgatccca tgcaataccg ctgattactt ttacctgtgc
     1201 atgataaaat aaaaatttcg gacgcttgtc aagtggtgaa gcaaaatatt catcgccttc
     1261 tataatcgtc accggtgcct gtgaaaattg caccatggta tcaaatcctt caagctgagc
     1321 gcctaccaga aaatcgaatg gcatctgcct ttttttcagc acatgcatga tcatactcgt
     1381 tatagaggtc ttaccatgac ttccacaaat agctacacgc aaactgtttt ttgattgttc
     1441 ataaacatat tccgggaaat tatacacccg tatgcccaat tcctgcgcac gcaataactc
     1501 aggattatcg gctcgcgcat gcatacccaa aatcactgca tccaattcac ttgttatgcg
     1561 ctccgggaac cagccaaaag cctccggtaa caagcctttc cgtgaaagtc gcgacttgct
     1621 gggttcgaaa atctcgtcgt cggaaccact aacctgatga cctaccgcat gcaatgccaa
     1681 cgccatattg tgcatcactg cccctccaat ggcgatcaaa tgtattcgca tggggtgaag
     1741 atatactaat tgctgaattt ggagatgtgg aaagcagtct tgtttgtgta aaatatattc
     1801 gtcatcttta cgttataata agtatgtaac cacatacaaa tgtgggaact gattaagcaa
     1861 accaatcgtt atcgttaaac aaaacattaa agcatatgcg taccttctca aaatgggctg
     1921 ttgcagccgg attatgcctt ctcctcacag ccggactcag ctcctgcggt cataaagaag
     1981 gttgccctaa taagatcact gaagttggcg gaatcaaaat tgaaaccaac gcctgatcgt
     2041 taataaatca ttctacaaag cctgaacctc cccggagttt caggtttttt ttatgcgtat
     2101 gagaaggtca ccactttaat catatctgaa gataaactct gtatcaccgg acaagtgcgt
     2161 gccgcatgtt ccaaaatggc tttttgcttg tcgttgaaat tgccgggaat aaacaaacga
     2221 cagtgtactt cgctgatacg gcgcgggttt gaggccatga tcttagtcac ttcaaccctg
     2281 gtgccttgaa tatccatttc gtgtgtccgt gccgctatgc ccataatcgt taacatacag
     2341 ctcgccaagg cagtagctac aagatctgtt ggcgaaaaag cttccccttt tccctgattg
     2401 tcgggtggtg catcggtgat gatgctcgtt ccggataagg tgtgcactgc tgttgtgcgc
     2461 aattcgcctt cgtaggtaac ttgtgatgta atcatgacag ttaatttttt gctttgggca
     2521 actatttatc cgcaaaaatc gtaatattat gcgatgaaat ttgttttctt ctgtatggca
     2581 tggatggcag tggtgtactc cgctgtcgct caggatacgt ctacagcgaa acaaaacact
     2641 tctaaacctg tcattctcaa caaaagttcg gcttctaaac ccaaaggact cagctatgaa
     2701 catgagttca gcatgggcgc aaaagtcaca actaccggtt ggggcatctt cggcgattat
     2761 accaaagaca tcgacctcga taagaagcgt gtagcctatt ttgaattgga attcctcaaa
     2821 gatcctaaag aaactaagcg tgtaaacgaa tttacagtcg gatacacccc tccaaaacct
     2881 tttgtatacg gcaaacagaa tacgtttttc aacctaaaag ctgccatcgg cgaaaaacgc
     2941 atgatagggg agaaggctga aaaaagtggc tttcaggtag ctgcaaatta tgccggcgga
     3001 tttagtgccg ggctcgtaaa accttattac ctggcggtgt acagtacctc cgaagacggc
     3061 acgccaacgg aaaatgtcaa atacacgccg gaaacggaat cgctgttttt agattatacc
     3121 tccatctacg gtgcctcagg ctttagcata gggtttaatg aaatatcctt tgtccccggt
     3181 ctctttggaa aggcaggtct caatttcgat tgggctacgt acgacgattt tgtaaagtcg
     3241 gcagaggcag gaatcggtgc agagctgtat ttgaaagata ttccaattat gatacttgaa
     3301 gataacaagc cttatttcgt gtatttatat ctaagtttgc agttcggtaa gaagtggtaa
     3361 aatagtggtg tcagcaacat gatcgaactt ccggttacag aatctcagga acgacagcga
     3421 aagccgaaat ggcttaaagt taaactcccc accggcgaaa attatcgcat ggtgcgcgag
     3481 cttgtcgatc agcataaatt acatactatc tgcgaaagtg gaaattgtcc aaacatgggc
     3541 gaatgttggg gtgaaggaac cgcaacgttc atgattttgg gtaacgtatg cacaagatct
     3601 tgctcttttt gcgctgtggc taccggccgc ccaactgagc tcgacctcga agaaccgttt
     3661 cgcgttgccg aagcaataaa gcttatgggc gtgaaacatg ccgttatcac ctcggtaaat
     3721 cgcgatgagc tggctgatag aggagccgct atctggaata gaacggtaac tgaagtgaag
     3781 cgcttaagtc cgcaaaccac cattgaaaca ctgattcctg atgttaaaag taattgggat
     3841 gccctggaga tgatgatatc ccccggacag gaagtggtaa gtcacaacat ggaaacggtt
     3901 aagcgcttat accgtctggt gcgacctcag gcaaaatatg aaagaagcct ggagcaaatc
     3961 aagcgcacaa aagactatgg caagcgcaca aaatcaggcg taatgctggg gcttggagaa
     4021 agtgc
//
LOCUS       DAOQHY010000246         3987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_13786_length_3987_cov_4.962614, whole genome shotgun sequence.
ACCESSION   DAOQHY010000246 DAOQHY010000000
VERSION     DAOQHY010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13786_length_3987_cov_4.962614"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..1235)
                     /gene="dnaJ"
                     /locus_tag="PLL28_13885"
     CDS             complement(81..1235)
                     /gene="dnaJ"
                     /locus_tag="PLL28_13885"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNF70467.1"
                     /translation="MAKRDYYEILGVGKQASAEEIKKAYRKMALQYHPDKNPGNAEAE
                     EKFKEAAEAYEVLSDQQKRARYDQFGHAGMGGAGGGAPHMNMDDIFSQFGDIFGESVF
                     GDFFGGGGGGRRQQRQRGVRGSNIRIKVKLTLKEIAEGARKTIKVKKYVVCDVCHGSG
                     AKDASSVTTCKTCQGSGYVRRVQNTILGQMATTQTCHTCHGSGQQVTANCTKCHGEGR
                     MYGEDTITLDIPAGVHEGVELSMNGKGNAGEKGGPNGDLLISVEEVKDPDLKREGNHV
                     LYDLYISFADAALGTKVEVPTIDGKVRVTIPEGTQGGHIMRLKGKGLPGLNSYSKGDQ
                     IIQVHIWVPKKLSSEERKMMEKLQESKNFIPDANETKKEKSFFDKMKDMF"
     gene            complement(1273..1833)
                     /locus_tag="PLL28_13890"
     CDS             complement(1273..1833)
                     /locus_tag="PLL28_13890"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNF70468.1"
                     /translation="MSETEQNKDQEVKNQAEQDVETPVNEQENLPVDEGAVDDATTMV
                     KELGEQKDKYIRLLAEFENYKRRTTKERLELFKTAGLEVIKDLLPALDDIDRAENAIA
                     SSKDIEAVKDGLKLMSDKLRNTLSQKGLKEIACKGLPFDTDTMEAITEIAAPTPELQG
                     KVVDVLEKGYTLNEKIIRYAKVVIGK"
     gene            complement(1955..2539)
                     /locus_tag="PLL28_13895"
     CDS             complement(1955..2539)
                     /locus_tag="PLL28_13895"
                     /inference="COORDINATES: protein motif:HMM:NF025273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNF70469.1"
                     /translation="MDSRLTSSHFTTSSIVYFFAALITLGFLSYGCSNSVTSPTIGVA
                     TGNHSPEIQSENLKGEMISLSAHKGSYVLVEFWDSQNSEARRNHFEMQRIYQKFKDAT
                     FDNGKKFCIYSVSLDTDREKWAKAVAEDNMSWPCNTIDQRAWNSPSVLAYNISYLPKY
                     YLIDGDGVIIRRNIMINELEALLNENLQGRLSNS"
     gene            complement(2541..3086)
                     /locus_tag="PLL28_13900"
     CDS             complement(2541..3086)
                     /locus_tag="PLL28_13900"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNF70470.1"
                     /translation="MSKHYFRATAIGGYILLFVMAIVLNSCKTPAPVVGDHIGNIAPE
                     ISAPDAQGKTQSLSALQGKLVLVEFWDSNNSASRKNHFEMQRMYSKYRNTSLDGGNGF
                     AIYSISIDTDPKKWQEAIAADGINFTTMVNDTQAWNSKAVLDYNIASLPKYFLIDDKG
                     IIINHNILIPELEKILIEQAN"
     gene            complement(3222..>3987)
                     /locus_tag="PLL28_13905"
     CDS             complement(3222..>3987)
                     /locus_tag="PLL28_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNF70471.1"
                     /translation="VLAEGKTTIYNAACEPYLQQLCDMLVRMGAKITGIGSNMLHIEG
                     VSTLGGCEHTILPDMIEIGSFIGLAAMTQSEITIRDVHFEQLGIIPEVFSRLGIAMER
                     RGDDLFIPAQEHYEIQNFIDGSILTIADAPWPGFTPDLISIVLVVATQARGSVLIHQK
                     MFESRLFFVDKLIEMGAQIILCDPHRATVIGLDRKFRLRGLEMSSPDIRAGVALLIAA
                     LSAEGKSIIRNIEQIDRGYQLIDQRLNNIGAQIVRI"
BASE COUNT         1022 a          966 c          762 g         1237 t
ORIGIN      
        1 tctcctttct cctttctcct ccgaatttaa attggtcact cctcattaac tcgcctgaat
       61 attctcccta aaagagactt tcagaacata tccttcatct tatcaaaaaa actcttttcc
      121 ttcttagtct cattagcatc gggaataaag ttttttgatt cctgcagttt ttccatcatc
      181 tttctttctt cagatgataa ttttttcgga acccatatat gcacctggat aatttgatca
      241 ccttttgaat aagaattcaa tccgggcagt ccctttcctt tcaaacgcat aatatgtccg
      301 ccctgtgtac cttcaggaat ggtgacgcgc actttaccgt caatggttgg cacttctact
      361 tttgttccga gtgcggcatc tgcaaagctg atatacaaat catacaaaac atgattgcct
      421 tcgcgcttga gatccggatc tttcacttcc tcaacactga ttaataaatc accattgggt
      481 ccgccttttt cccctgcatt accttttccg ttcattgata actccacccc ttcatgcaca
      541 ccggcaggaa tatcgagcgt gatagtatct tcaccataca tgcgaccttc accatggcat
      601 ttcgtacaat ttgctgtaac ctgttgtccg gaaccatggc aagtatgaca tgtttgtgtg
      661 gtagccattt gtccgaggat ggtattttgc actctgcgca cataacctga tccctgacaa
      721 gttttacaag tagttacact cgaagcatct ttagcaccgc tgccatgaca tacatcgcac
      781 actacatatt tttttacttt gatggttttg cgggcacctt ctgcaatttc cttcagtgtc
      841 aattttacct tgatgcggat attgctacct ctgacaccgc gctgacgttg ctgtcttctt
      901 ccaccaccgc cgccgccaaa gaaatcgcca aaaactgatt caccaaagat atctccaaac
      961 tgactgaaga tatcatccat attcatgtga ggcgcaccgc cacctgcacc acccattccg
     1021 gcatgaccaa actgatcgta gcgtgcacgt ttttgttgat cgctcaacac ttcgtatgct
     1081 tcagcagctt ctttaaattt ctcttcagct tctgcattac ccggattttt gtcagggtga
     1141 tattgcaaag ccatcttgcg ataagctttt tttatctctt cggcagaagc ttgtttgcca
     1201 acacctaata tttcgtaata atctcttttt gccatgattg cgccggtagt tcgttgtggt
     1261 gattttcgtt tattatttac ctatcaccac ttttgcgtag cgaataattt tctcgttcaa
     1321 tgtgtacccc ttttccaaca cgtctaccac cttaccttgc aactcaggcg ttggagcagc
     1381 aatttcagtg atggcctcca tagtatcggt atcaaagggc aatcctttac atgcaatttc
     1441 tttcagtcct ttttgagaaa gtgtatttct aagcttatcg ctcatcaatt tcaagccgtc
     1501 tttcacagct tctatatctt tcgatgaggc gatagcattt tcagcacggt cgatatcatc
     1561 caaggccgga agaaggtctt tgattacctc caagcctgca gttttaaaaa gttccagtcg
     1621 ctccttggtg gttctgcgct tatagttttc gaattctgcc aagagacgga tgtacttatc
     1681 cttctgctcg ccaagttcct tgaccatagt ggtagcgtca tcaacggctc cctcatccac
     1741 aggcagattc tcctgctcgt ttaccggagt ttctacgtcc tgttctgcct gatttttcac
     1801 ttcttgatcc ttattttgct ctgtttcgct cataattgcc ggttattcat gtcaaaaatg
     1861 catacccctt ccgttcccaa aaggttaatt ggggtgggca aaaatactgc cactgcactg
     1921 tcatttgcca aaatgacacg aaaaaaccgc caaatcagct gttagagagc cttccctgca
     1981 ggttttcgtt caggagagct tcgagttcat tgatcatgat attgcgacga atgatcacgc
     2041 catcgccgtc aatgaggtag tatttgggta gataactgat attatatgcc aacacagacg
     2101 gagagttcca ggcacgttga tcgatagtat tacatggcca actcatgttg tcttctgcaa
     2161 ctgctttcgc ccatttttcg cgatcggtat ccagagaaac cgaataaatg cagaatttct
     2221 tcccgttgtc gaaggtggca tccttgaact tctgataaat acgctgcatt tcgaagtggt
     2281 tacgtcgagc ttcagaattc tgtgaatccc aaaattctac caatacatag gaacccttat
     2341 gagcagacaa gctgatcatt tcgcctttca gattttctga ttgaatttca ggtgaatggt
     2401 taccggtagc aacacctatg gttggtgagg tgacggaatt actgcatccg taggaaagaa
     2461 acccgagggt tattaaggct gcgaaaaaat aaacaattga cgatgtggtg aaatgggagg
     2521 aggtcaatct gctatccata ttaatttgct tgttctatta gtatcttttc cagttcgggt
     2581 atcaggatgt tatggttaat aataattcct ttatcgtcga tcaaaaagta cttcggaagt
     2641 gatgcaatat tgtaatccaa gactgctttg ctgttccatg cctgagtatc atttaccata
     2701 gtggtgaaat tgataccgtc ggcagcaatc gcctcttgcc actttttagg atctgtatct
     2761 attgagatgc tataaattgc aaaaccgttg cctccatcga gagaagtatt gcgatattta
     2821 ctatacatgc gttgcatctc gaaatggttt tttcttgaag ctgaattatt ggaatcccaa
     2881 aattccacca atacaagctt gccttgcaat gcgcttaggg actgcgtttt accttgagca
     2941 tcgggagctg atatctcagg ggcgatatta cctatgtggt cgccaacaac tggcgccggt
     3001 gtcttgcagg aattgagcac tattgccatt acaaacagga gtatataccc accaatggca
     3061 gtagccctaa aataatgttt actcatctga aacggtgtgt gtgaagaaaa tgggaaccgt
     3121 tgaatcgagc acaaaattaa tcatgcgccg ataattaatt ataatttatt accggtcatt
     3181 atcaagaatt aacacatacg catgcaaatc gccgtcatcc gtcaaatcct tacaatctga
     3241 gcgccgatat tatttaatcg ctgatcgatt aattgataac ctctgtcgat ctgctcaata
     3301 ttgcggataa tgctttttcc ttctgcagat aatgccgcta tcagcaatgc caccccggca
     3361 cgtatatccg gagaactcat ctccagtcca cgcaatctga acttcctgtc caaaccgatt
     3421 actgttgccc gatgcgggtc gcataagatg atctgcgcac ccatttctat cagcttgtcg
     3481 acaaaaaaca atctgctttc aaacattttc tgatgaataa gcacgcttcc tcttgcttgc
     3541 gtagccacca ccaaaacgat actgataagg tcgggcgtaa atcccggcca aggtgcatcg
     3601 gctatggtaa gtattgatcc gtcgatgaaa ttctgtattt cgtaatgttc ttgagcaggt
     3661 atgaaaaggt catccccacg gcgttccatg gcaattccca atcttgaaaa cacttccggg
     3721 atgataccaa gttgctcaaa atgtacatcg cgaatggtga tttcactctg tgtcattgcc
     3781 gccagtccga taaaactgcc gatttcaatc atgtcgggaa gaatagtatg ctcacatcca
     3841 cctaaggtac tcaccccttc aatatgcaac atatttgagc ctatgcccgt aatctttgct
     3901 cccatgcgca ccaacatatc gcagagttgc tgcagataag gttcgcaggc agcattgtag
     3961 atagtagttt tgccttcagc caatact
//
LOCUS       DAOQHY010000247         3972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_13873_length_3972_cov_3.354863, whole genome shotgun sequence.
ACCESSION   DAOQHY010000247 DAOQHY010000000
VERSION     DAOQHY010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13873_length_3972_cov_3.354863"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..552
                     /locus_tag="PLL28_13910"
     CDS             265..552
                     /locus_tag="PLL28_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70472.1"
                     /translation="MTKDINNFAEHVTQWEQSGLSMAAYCKAQGLNYQTFTYHVGRKK
                     KKAAETAATGSFTRIEVPVQNAAGIEYHLPNGGYFVFPAGCSVQMIKALVG"
     gene            552..893
                     /gene="tnpB"
                     /locus_tag="PLL28_13915"
     CDS             552..893
                     /gene="tnpB"
                     /locus_tag="PLL28_13915"
                     /inference="COORDINATES: protein motif:HMM:NF017528.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HNF70473.1"
                     /translation="MLSLSNHQRYFLYRDVVDMRKGIDGLSGLVRDYLKLDPLSGDVY
                     LFLNRKRNQVKLLMWDKDGFALYYKRLEAGTYELPVGSDTAIGMDVLLCILQGIRLTA
                     MRKRKRYEQAT"
     gene            987..2477
                     /locus_tag="PLL28_13920"
     CDS             987..2477
                     /locus_tag="PLL28_13920"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HNF70474.1"
                     /translation="MTEERGYELLKENAELKSELLMIKQEMTQLRRMIFGSKSERFVP
                     AQNPDQLSLDITPEEPSEVTAIPQKKKIEAYDRKVPSDQKIHPVRQGFPEHLPREITI
                     IKPEGDLSGCKKMGEEITEVLDLIEPKFFVKRFVREKYVHEQTGKISIGELPSRAIDK
                     SLFGENLIAQIITNKYVDHLPLYRQIERFKRDGMTLSASTLNDTVKNSCGLLKPLYQH
                     LREQVLSDDYLQVDETPIKVLDPSTKGKTHRGYHWVYHAVNKQLILFDYRQGRGREGP
                     EELLKNFSGYLQTDGYQVYDAFTKRKDIILVGCMAHVRRKFDEARQNDQNLAENALLL
                     IQRLYIIEKAIREHSGISDAEIMRLRQEMSLPTLQDFENWLKLHLTDTLPKSAIGQAI
                     QYALPRMEKLRRYTEQSYLQIDNNLVENAIRPVAVGRKNYLFAGSHEGAERAAMIYSF
                     FGTCKLNNINPQHWLADILTKINNTKTSQLENLLPANWKVANTKNY"
     gene            complement(2506..>3972)
                     /locus_tag="PLL28_13925"
     CDS             complement(2506..>3972)
                     /locus_tag="PLL28_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70475.1"
                     /translation="FANSFVNCSDFNYPVGIKSVNATYTVHSELCIPPAEVGCEVEIS
                     SFTGYYRAIWAANTAYRPLKIRVEGVDFIDNERAMLFSAVSNSEIWRNNFEIPDYDSK
                     TGYGLYLEGCINYHVEGNYFTSYGSMTNSSHYCSGIFVENNHNTATEIYRNSFDNIEI
                     GIRSQGNNSKLQIRCNDFNTPILDYNIIVTSGTLGNQGICNATITQPAGNQFSHDDFT
                     ESDFRIVPSGVNLNYRHHTEATYIPEDYTTTQINLHNCSVSGAEDCVSTLPGGTGGEE
                     RMMMTKATEYQAAIELELAKIDGGNTGGMITDIQGGASQTEVKYALDEASPYISSEVL
                     VALVNEEPLGTTEMLDVMDSNYPISDTVATSLEISNYDVPSAVIETLSEDIVDNMLTV
                     SAMSDLLATVSVLETKREYAVLQSINHLVDEIEIDSAIALVSEETGDWARQQEVQILT
                     SISNYSQAESALEQYSSVDQFGEDFKTLYTIINDIEST"
BASE COUNT         1197 a          816 c          841 g         1118 t
ORIGIN      
        1 aaaatggcat gtcgcctgaa aacaaagaag tccagcaagt aacacgggta gctgttgcac
       61 aactctttag cgtttctgtt tagcgcggta ttgggtgtat catcgcggca gtttttaatt
      121 aaatcgttgc actggttttt ctaactattg ccactattag ggcattacag agtcaaaagg
      181 gcggaaaaaa tatggcgctt gattcgtaac catccggtaa actgcatatt gttttgttaa
      241 aagtatttag ttcgctttgc atgcatgact aaagatatca ataatttcgc tgagcatgtc
      301 actcagtggg agcaaagtgg cctgtcgatg gcagcatatt gcaaagcgca gggtcttaat
      361 tatcaaacat ttacttacca tgttgggcgg aagaagaaaa aagcggctga gactgctgca
      421 accggttcgt ttacccgtat tgaagtgcct gtgcaaaatg cagccgggat agaataccat
      481 ttacctaatg gtggctattt tgttttcccc gcaggttgtt ccgttcaaat gatcaaggcg
      541 ttggttggtt aatgctttcg ctgagcaatc accagcggta ttttttgtat cgcgatgtag
      601 tggatatgcg caaaggcatt gatgggttga gcggactggt gcgggattat ttgaagctcg
      661 atccactatc gggagatgtt tatcttttcc ttaaccgcaa gcgcaaccag gttaaactgt
      721 tgatgtggga taaggatgga tttgctttat actacaaacg cctggaagcc ggcacctatg
      781 agttacccgt gggctcggat acagccattg gcatggacgt gttgctgtgt atcctgcaag
      841 gcatcagatt aacggccatg cgtaaacgca aacgctacga acaggcaaca taagcttgga
      901 ttattggcat acaatgtgga ttaatcggtg tacatcttta ctagataaaa ttgcttatac
      961 aacatgcaat aaactacctt tattgcatga ccgaagaacg tggttatgag ttgttaaaag
     1021 agaatgcgga gttgaaatca gaactgctga tgatcaagca ggaaatgacg caactcaggc
     1081 gtatgatctt cggttcaaag agcgaacgat ttgttcccgc acagaacccc gatcaattaa
     1141 gcctggatat tacacctgaa gaaccgtcgg aagtgaccgc gataccacag aaaaagaaaa
     1201 tagaggcgta tgatagaaag gtgccgtcag atcagaaaat ccatcctgtt cgccagggct
     1261 tccctgagca tcttcccaga gagataacca ttatcaagcc tgaaggtgat ttatcgggat
     1321 gtaaaaaaat gggagaagaa atcaccgaag tgctggatct tatcgagccg aagttttttg
     1381 taaaacgatt tgtacgcgaa aagtatgtgc acgagcagac cggaaagatc agtatcggcg
     1441 aactaccatc cagagcgatt gataaaagct tgtttggcga aaacctgatc gcacagatta
     1501 ttaccaacaa atatgtggat caccttcccc tgtatcgcca gatagagcga tttaagcgcg
     1561 acgggatgac attatctgca tccaccctga atgatacggt aaaaaatagc tgcggcctgc
     1621 taaaaccctt atatcagcat ttacgggagc aggtattatc agatgattat ctgcaggtgg
     1681 atgaaacacc gataaaagta ttagatccgt ctacaaaagg caaaacacat agagggtatc
     1741 attgggtgta tcatgcggta aataagcaac tcatattatt tgactatcgc cagggccggg
     1801 ggcgagaagg tcctgaggag ttgttaaaaa acttctcggg ttatttacaa accgatggat
     1861 atcaggtata tgatgctttt accaaaagaa aggatattat ccttgttggc tgcatggcgc
     1921 atgtgcgcag gaaatttgat gaagccagac agaatgatca gaatctggcc gaaaatgcat
     1981 tgctgcttat ccagcgatta tatatcatcg aaaaagcaat ccgcgaacat agtgggatta
     2041 gcgatgcaga aatcatgcgc ctcagacaag agatgtcact tccgacatta caggattttg
     2101 aaaactggct gaagctgcat ctgaccgata cattaccaaa gtcagccatc gggcaagcca
     2161 tccaatatgc actgccgcgc atggaaaaac tacgtcgata tacagagcag tcttatttgc
     2221 aaatagataa caatctggta gaaaatgcta tccgtccggt agctgtgggc agaaagaatt
     2281 atttatttgc cggctcgcac gaaggcgccg agcgtgcagc aatgatctat tccttcttcg
     2341 gcacctgcaa gctgaacaac ataaatccgc agcattggct tgccgacatc ctcaccaaaa
     2401 tcaacaacac caaaacctcg caactcgaaa acctcctccc agcaaactgg aaagttgcaa
     2461 atacaaagaa ttattgagta tttttagatg tggttgaccg agtggttacg tactttcaat
     2521 atcattgata attgtgtata gcgttttgaa atcttcgcca aattgatcaa cacttgaata
     2581 ttgctccaga gcagattcag cctgactgta attagatatt gaagtgagaa tttgaacttc
     2641 ttgctgacga gcccaatctc ccgtttcttc tgatacgagg gctatcgctg agtcgatttc
     2701 aatttcatca acaagatgat ttattgattg caagacagca tattcacgct tggtttcaag
     2761 tactgataca gttgccaata aatctgacat ggctgatacc gtaagcatgt tgtcaacaat
     2821 atcttcgcta agtgtttcaa tcactgcaga tggcacatca taattgctaa tttcaagtga
     2881 ggtagcaact gtatcggaaa taggataatt gctatccata acatcaagca tttcagttgt
     2941 acctagcggt tcttcattga ccaatgcaac cagtacttca ctgctaatat atgggcttgc
     3001 ctcatcgagt gcatatttaa cttcagtttg tgaagcccca ccttgtatat cagttatcat
     3061 cccgccggta tttccaccat caatctttgc aagttctagt tcaattgcag cctgatattc
     3121 agtagcttta gtcatcatca ttctttcctc accacctgtc ccgcccggga gcgtggatac
     3181 acaatcctca gcgccactaa cactacaatt atgtagatta atttgtgtag tagtatagtc
     3241 ttctgggata taagtcgcct cagtatgatg tctataattt aaattaacac cagaagggac
     3301 aatcctaaaa tcactctcgg taaaatcatc atgactaaat tgatttcccg ctggttgtgt
     3361 tatagttgca ttacaaatac cttgatttcc taatgttcct gatgtaacaa taatgttgta
     3421 atcaagaata ggtgtattga aatcattgca acgtatttgt aactttgaat tgtttccttg
     3481 actgcgaatc ccaatttcaa tattatcaaa cgaatttcta tatatttctg tcgcagtgtt
     3541 atggttgttt tctacaaaga taccagaaca ataatgggaa ctatttgtca ttgagccgta
     3601 actagtaaaa taatttcctt caacgtggta attaatacat ccttctagat acaatccata
     3661 acctgtctta ctatcgtagt caggaatttc aaaattgtta cgccaaattt ctgaattcga
     3721 aaccgcggag aacaacatcg cccgttcatt gtcaataaaa tctactcctt ctactcttat
     3781 tttcagcggg cgatatgcag tgttagcggc ccatattgca cgataatatc cggtaaaact
     3841 tgatatttca acctcacatc caacttctgc tggagggatg caaagttcac tatgaacagt
     3901 ataagttgca tttacacttt tgattccgac agggtaatta aaatcactgc aattgacgaa
     3961 tgaatttgca aa
//
LOCUS       DAOQHY010000248         3933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_14076_length_3933_cov_4.872357, whole genome shotgun sequence.
ACCESSION   DAOQHY010000248 DAOQHY010000000
VERSION     DAOQHY010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14076_length_3933_cov_4.872357"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1421
                     /locus_tag="PLL28_13930"
     CDS             <1..1421
                     /locus_tag="PLL28_13930"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNF70476.1"
                     /translation="FKQYINGAWCDAQNAGTLNVIDPANGEYVQTVPYGDGNDTTIAI
                     DAAHDAFRTWKKMSPWTRADILQKTANYIRQHIVEFATLMVRESGKPLAEAKGEWTVA
                     ANLFEWYAEEGKRAYGTVIPSARTDKRMQVILQPMGVIGVITAWNFPAYNPARAWAAA
                     LAAGCTIVAKVSEFTPLSGMLLINALEASGIPKGVVNLVNGDAANTGKILLQDARVRK
                     ISFTGSTRVGKILMSGAAETNTKLALELGGNAPVIIMPDVDIDHVAKTAVAGRFRNCG
                     QVCIAPQRFFVHADIIDAFQERVVYYTAQLNVGHGLEPETRIGPLINSIQQDHVLSII
                     TEAKAEGNEVLTGGHSIAGGHFVAPTVIRVKNVASVFYDREIFGPVMPLIPFNDIDEV
                     IKSANETPYGLAAYVWTNNINHATYISEALEFGIVGINEWAAHATEAPFGGWKQSGLG
                     HESGREGLLEYMEKKLISIGGL"
     gene            1478..2878
                     /locus_tag="PLL28_13935"
     CDS             1478..2878
                     /locus_tag="PLL28_13935"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HNF70477.1"
                     /translation="MEHLATDYGWLALIPPLVVIIVSIMLKSSFEGLLIGCVTGFIIV
                     DGWGFFDGFITSLYAVMQSTDTIWVIVVCGLYGSVMAMMIRSGGAMQFGERMLRYMKT
                     KRQALMGTWLLGIFVFIDDYLSALTTGITMRAVTDKFKIPREYLAYIVNTLSPPLCVI
                     VPISTWTLFIGVQLENSGFAAEGQGLQKYMSVIPFVAYGWISIFMVPLFIYGILPHFK
                     KMKQAIARAEETGLTVPVGIKLSTVQFETFPGLVHPKARYFLLPMIVVLGATVAFQDP
                     SVPGIEIDALKGVIIGVAFTFFYFLYMKLSNVQHLTETIFSGFNSMLFALALVVVSLM
                     LKDVGDKMGLTQYVINSVTPYVSSAFLPVSIFIALSLISFTTGSSWGLYVVAIPIVIP
                     LAQSIDSNVLLNIGAIISAGVFGANACLYSDATVLTSQSCETNNMQHGVSQLPYALTS
                     WSVSVLAYIILGYVMA"
     gene            2889..3524
                     /locus_tag="PLL28_13940"
     CDS             2889..3524
                     /locus_tag="PLL28_13940"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNF70478.1"
                     /translation="MEIINEIISGNIIIIHTRAEHAQALEDLQYIVFPNLAEDEILHK
                     AHYLKHLEIFPEGQLVALHNDQVIGGTTTMRYHMDPDHPAQHTFSETVAGGWLTTHQP
                     AGEWLYGIDVSVHPDYRNRGIARAFYNIRQQIAKALGCRGQLTVGMLNGYFNYASTMS
                     VKTYFEKVKAHELFDPTVSVQEKIGFRIVSLMENYLNDPTCGNAGAVIVME"
     gene            3535..>3933
                     /locus_tag="PLL28_13945"
     CDS             3535..>3933
                     /locus_tag="PLL28_13945"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNF70479.1"
                     /translation="MSTIQLKTSIPGPKSQEIIERRKNAMPAGLAKSTEVAVVSAEGG
                     VVTDADGNILLDFAGGIGMMNIGHRHPDVVKAMQEQLDKYIHVCALVATPEPYVELAE
                     MLNALTPGNFPKKTILANSGSEAVENAINIA"
BASE COUNT         1148 a          758 c          907 g         1120 t
ORIGIN      
        1 agtttaaaca atatattaat ggcgcctggt gcgatgcaca aaatgccggc acactaaacg
       61 taattgatcc cgctaatggt gagtatgtgc aaactgtgcc ttacggcgat ggcaatgata
      121 ctacaattgc cattgatgcg gcacatgatg catttagaac atggaagaaa atgtcgccat
      181 ggacaagagc tgatatcctg caaaaaacag cgaattatat ccgccaacat attgttgaat
      241 ttgcaacttt aatggtcaga gaaagtggta aaccgctggc tgaagcgaaa ggcgaatgga
      301 ccgttgccgc aaatttattc gaatggtatg ccgaagaagg caaacgtgct tatggcactg
      361 taattccatc tgcaagaaca gataaacgca tgcaggtaat tctgcaacct atgggtgtta
      421 tcggtgtcat tactgcctgg aatttccctg cttataatcc tgcccgtgcc tgggctgccg
      481 cattagccgc cggttgtacc atagttgcta aagtgagcga gtttacacct ctttccggca
      541 tgctcttaat aaatgcgctc gaagcatcgg gaattcctaa aggcgttgtc aaccttgtaa
      601 atggcgatgc tgctaataca ggaaaaatat tattgcaaga tgcacgtgtg cgaaaaatta
      661 gttttacagg aagtactcgc gtaggcaaaa tattaatgag tggtgctgcc gaaacaaata
      721 cgaaacttgc cctcgaactt ggaggcaacg ctccggtaat tattatgcct gatgtcgata
      781 tagatcatgt tgcaaaaaca gctgtggcag gtagatttag aaattgcgga caagtatgca
      841 tcgcaccaca acgatttttt gtacatgccg atataattga cgcctttcag gaacgcgttg
      901 tctactatac cgcgcaatta aatgttggtc acggtcttga gccggaaacg cgcatcggac
      961 cattgatcaa tagcattcaa caagatcatg ttttgtcaat aatcaccgag gctaaagcgg
     1021 aaggaaatga agtattgaca ggaggtcata gcatagcagg cggacatttt gttgcgccaa
     1081 ctgtaattcg cgtaaaaaat gtagcatccg tattttatga tcgagagata tttggtccgg
     1141 ttatgccgtt gattccattc aatgatatcg atgaggtaat taaatcggca aatgaaacac
     1201 cttatggatt ggcagcttat gtatggacga acaatatcaa tcacgccacc tatatcagtg
     1261 aagcattgga atttggtatt gtcggcataa atgaatgggc cgctcatgct actgaagcgc
     1321 catttggtgg ctggaaacaa agcggtttag gtcatgaaag cggtagagaa ggcttgttgg
     1381 aatacatgga aaagaaatta atcagtatcg gcggactatg aagtctagac tacaccaata
     1441 gtagtctaaa ctgaaaaaat gcaaaaacac acctgaaatg gaacacctgg caacagatta
     1501 cggatggctg gcactgatac cacctttagt ggtgatcatc gtttccatca tgctcaaatc
     1561 atcattcgaa ggtttgctca tcggttgcgt aacaggattt atcatcgtcg acggctgggg
     1621 attttttgat ggatttatca cttcattata cgcagtaatg caatcaacag acaccatctg
     1681 ggttattgtt gtttgcggat tgtatggctc cgttatggca atgatgattc gcagtggtgg
     1741 cgccatgcaa tttggtgaac gcatgttgcg atacatgaaa acaaaacgtc aggcattaat
     1801 gggcacatgg ttgctgggca tttttgtttt catcgacgac tatctcagcg cactcacaac
     1861 aggtatcacc atgcgtgctg ttaccgataa atttaaaatc ccgagagaat atctggcata
     1921 tattgtaaat accttatcgc caccattatg tgttatcgtg ccgatttcaa cgtggacatt
     1981 atttattggc gtacaactcg aaaattccgg atttgccgct gaaggtcaag ggctgcagaa
     2041 atatatgagt gttattccct tcgttgccta cggatggata tctatattca tggtaccgct
     2101 ttttatttac ggaattttac ctcatttcaa aaagatgaag caagcaattg cgcgtgctga
     2161 ggaaacaggg ttaaccgtac ctgttggtat taaattgtcg acagtgcaat ttgaaacatt
     2221 tccgggactg gtacatccaa aggcgcgata ttttttatta cctatgatcg ttgtattagg
     2281 tgctacagtt gcctttcagg atccgtcggt gcccggaatt gaaattgatg cgctaaaagg
     2341 tgtgatcata ggtgtagcat ttacattctt ttattttcta tatatgaagc tgtcgaatgt
     2401 acagcatctg acagaaacaa ttttcagcgg atttaactca atgttgtttg cactggcact
     2461 tgttgtagtg tcgctgatgt tgaaggacgt cggtgataaa atgggactta cacaatatgt
     2521 cataaatagt gttactccct atgtcagcag tgcatttttg ccggtgtcga tttttattgc
     2581 attgagttta atatccttta ctaccggttc aagctgggga ttatatgtag tggcaattcc
     2641 tattgtaatt ccgcttgcgc aaagtattga ttcaaatgta ctattgaata tcggcgctat
     2701 aatcagtgcc ggtgtgtttg gcgccaatgc atgtttgtat tccgatgcca cagtgctcac
     2761 atcgcagagt tgtgaaacaa ataatatgca acatggagta tctcaattgc cttatgcact
     2821 cacctcttgg agtgtgagtg tgttggcata tattattttg ggatatgtaa tggcgtaaag
     2881 aaattgtaat ggagattatt aacgaaataa taagtgggaa catcatcatc atacataccc
     2941 gtgctgagca cgcgcaagca ttggaagatt tgcaatacat agtatttcct aatcttgctg
     3001 aagatgaaat cctgcataaa gcacattacc tgaagcattt ggaaatattt cctgaaggtc
     3061 aattagtggc attgcataac gaccaggtga taggagggac taccaccatg cgatatcaca
     3121 tggatcccga tcatccggct caacatacat tttcagaaac agtagcaggt ggatggctca
     3181 ccacacatca acctgccggt gaatggttat atggcatcga tgtaagtgtg catcccgatt
     3241 atagaaacag aggtattgcc cgcgcatttt ataatattcg tcagcaaatt gccaaggcgc
     3301 ttggttgtcg cggtcagctg actgttggta tgttgaacgg gtatttcaat tatgcaagta
     3361 ctatgtcagt gaaaacgtat ttcgagaaag taaaagccca tgaattattt gatcctaccg
     3421 tttcagtgca ggagaaaatt ggtttcagaa ttgtcagctt aatggaaaat tatttaaacg
     3481 atcccacttg cggaaatgca ggtgcggtta ttgtaatgga ataaaataaa tgatatgtca
     3541 accattcaac taaaaacaag tatccccggt ccgaaatcac aggaaattat tgaacgtcgg
     3601 aaaaatgcca tgcctgcagg tttggccaaa tctaccgaag ttgccgtagt ctccgctgaa
     3661 ggtggtgttg ttacagatgc tgatggcaat attctgctcg attttgccgg tggtatcggc
     3721 atgatgaata tcggtcatcg gcatcctgat gttgtgaaag cgatgcaaga acagttggat
     3781 aaatacattc acgtttgcgc attagtagcc acacccgaac catatgttga actggctgaa
     3841 atgttgaatg cattgactcc gggtaatttc ccaaagaaaa caatcctcgc caacagcggt
     3901 agcgaagcag tggaaaatgc cattaatatt gcg
//
LOCUS       DAOQHY010000249         3910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_14217_length_3910_cov_5.654734, whole genome shotgun sequence.
ACCESSION   DAOQHY010000249 DAOQHY010000000
VERSION     DAOQHY010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14217_length_3910_cov_5.654734"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PLL28_13950"
     CDS             complement(<1..377)
                     /locus_tag="PLL28_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70480.1"
                     /translation="MSCAGCAVGTDKSSPAGCKSNGHCATGGCNKLNTFDWLRAMPVP
                     VSQRFNIHEVSFKNGVRKGFYANSKGIDVMTGDMVCVETESGYDVGRISLSGELVRLQ
                     MKKRRVSPNSTFSPILRKATDAD"
     gene            complement(389..1567)
                     /locus_tag="PLL28_13955"
     CDS             complement(389..1567)
                     /locus_tag="PLL28_13955"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70481.1"
                     /translation="MFFSQVIGQDRIKQQLIRAVEEGRVSHAQLFLGPEGCGNLAMAL
                     AFAQYINCENRQTGTDSNGKVTGDSCGKCPSCIKAQKVIHPDIHYTFPFVKIEDKELC
                     ADWMKEWREFVLQTPYGTYNDWIENITEGNKQGNITAKECLEILRKLSLKNYEGIYKV
                     QILWMPEFLDKEGNRLLKMLEEPPDNTVFLLVANDSEQILNTIMSRVQIVKFARLADE
                     DVAQVLERDHQQPHNQAVKIAQMAGGNLQEAIRLLDIHEDANTAMIREWMEACYRQQV
                     KQLFAWNDKFLKLGREQQKMFFQYCLHFFREVMLIQAGAGQLAKLTDAELKTAQGLGQ
                     VLGVEKLMGIIELFDKSIYFVERNVNGKIMMTYVSVTLLKQFQKNYQPTRPFYDRWAL
                     "
     gene            complement(1585..2073)
                     /locus_tag="PLL28_13960"
     CDS             complement(1585..2073)
                     /locus_tag="PLL28_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNF70482.1"
                     /translation="MQEKSSLHAFQFPSIDGGMIDFAVFKGKKVLVVNTASDCMYTDQ
                     YKQLQELSVTYPNELVVVGFPSNDFGEQEPGTNREIKNFCSYRYGVTFPLASKSPVIG
                     MDANPVFKWLTTVPFKTDMNKTVTWNFQKFLCDENGVLIEVYPPAADPINDGLLEHIN
                     PN"
     gene            2139..2546
                     /locus_tag="PLL28_13965"
     CDS             2139..2546
                     /locus_tag="PLL28_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNF70483.1"
                     /translation="MKTSVIRRATFNAAHRLHNPAWDDATNSKVFGACNNPNYHGHNY
                     TLFVKITGEVDPVTGYVMDLKDVNILINTYVIEPLDHQNLNLDVPDFRNLNPTAENIA
                     RIIYEKLRSALAADLELKVTLYETENNIVEYPG"
     gene            complement(2554..2922)
                     /locus_tag="PLL28_13970"
     CDS             complement(2554..2922)
                     /locus_tag="PLL28_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HNF70484.1"
                     /translation="MQDITSRSDVAKIVVEFYNDVRRDELLAYIFNDVAQVNWETHTP
                     LIIDFWEYTVLGTGSYTRNAMTPHVALHAKSPLTQAHFDRWLQLFHATVDKYFAGENA
                     DLMKTRADGIAGLMHHKLNG"
     gene            complement(2922..3320)
                     /locus_tag="PLL28_13975"
     CDS             complement(2922..3320)
                     /locus_tag="PLL28_13975"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNF70485.1"
                     /translation="MTDFISIEATLGAENYKTSMHNGRSTVFCDEPADKGGKDSAPAP
                     RELLCMSLAACTAITLRMYAERKNWALGNIDVQVTLSEGEAGKQVFNRTIKPEHKQDQ
                     AIYDRLLMVADKCPVHKLLAGTNEIITTIA"
     gene            3496..>3910
                     /locus_tag="PLL28_13980"
     CDS             3496..>3910
                     /locus_tag="PLL28_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF70486.1"
                     /translation="MDAVISTDIRELNERIHQESAFIDLLRMETSKAIVGQKYMIDRL
                     LLGLLSQGHILLEGVPGLAKTLAIKSLSSAVNAKFSRIQFTPDLLPADLIGTMIYNQK
                     DNNFSVKKGPIFANFILADEINRAPAKVQSALLEAM"
BASE COUNT         1069 a          910 c          793 g         1138 t
ORIGIN      
        1 atatcagcat cggtagcctt tcttagaatg gggctgaaag tgctgttagg cgaaacacga
       61 cgttttttca tttgtagtcg caccaattcg ccgctgagtg aaatcctccc aacatcgtaa
      121 ccgctttctg tttccacgca aaccatgtcg cccgtcatca catctatgcc tttactattg
      181 gcataaaatc ctttgcggac gccattttta aaacttactt catggatatt gaaacgctgt
      241 gaaacaggta ccggcatagc gcggagccaa tcgaaagtat tcagtttatt gcatcctccc
      301 gtagcgcagt gcccgttgct tttacaaccg gccggggagc ttttatccgt gcccacagca
      361 catcctgcac aactcatagt aatatatttt aaagggccca ccgatcatag aagggccgcg
      421 ttggctgata gttcttttga aactgcttta acagtgtcac cgaaacatac gtcatcatga
      481 tcttgccgtt aacgttccgt tccacaaagt aaatactttt gtcaaataat tcaataatac
      541 ccatcagctt ctcaacccca agcacctgtc ctaatccctg ggcagttttc aactccgcgt
      601 ccgtgagttt ggcgagttgc cctgcgccgg cctgaataag cattacctcg cggaaaaagt
      661 gcaggcagta ttgaaaaaac atcttctgct gctcccttcc cagctttaaa aacttgtcgt
      721 tccacgcgaa taactgcttt acctgctggc gatagcaagc ttccatccac tcccggatca
      781 ttgcggtatt agcatcctcg tgaatatcta acaaacgtat agcctcctga aggttaccac
      841 ctgccatctg agctatctta actgcttgat tatgaggctg ttggtgatcc cgctccaaaa
      901 cttgtgccac atcttcatca gcaagtcgcg caaatttgac gatttgcacc ctagacataa
      961 tggtattcaa tatctgttcg ctgtcattgg caacaagtaa gaaaaccgta ttatcaggcg
     1021 gttcttccag catttttaac agacggttgc cttccttgtc gagaaactca ggcatccaga
     1081 gaatctgcac tttataaatg ccttcatagt tcttgagtga aagcttgcgg agaatctcca
     1141 ggcactcttt ggcggtgata ttgccttgtt tattgccttc agtaatattt tctatccaat
     1201 cgttataagt gccatatgga gtttgcaaca caaattcccg ccattccttc atccaatcgg
     1261 cacatagttc tttgtcttct attttgacga agggaaaagt gtagtgaata tcaggatgga
     1321 tcactttttg cgccttgata caggaggggc atttgccgca ggaatcgcct gtcaccttgc
     1381 catttgaatc agtgccagtt tggcgatttt cgcaattgat gtactgagca aaagccagtg
     1441 ccatagccag attaccgcag ccttcagggc ccaaaaacaa ctgggcatgg ctcacccgtc
     1501 cttcttcaac agcgcgaatc agctgctgtt tgatgcgatc ttgtcctatg acctggctga
     1561 agaacatccg aagattttat gttatcaatt cgggttaata tgttccaaaa gcccatcgtt
     1621 tataggatca gctgccggag gatatacctc tataagcacc ccgttttcat cgcaaagaaa
     1681 cttttggaaa ttccatgtca ccgttttatt catgtcggtc ttaaacggaa cagttgtaag
     1741 ccacttgaac accggatttg catccatccc aatcaccgga cttttcgaag ccaggggaaa
     1801 ggtgacgcca tagcgatatg aacagaaatt ttttatctca cggtttgttc cgggttcctg
     1861 ctcaccaaaa tcattcgacg gaaagcctac caccaccaat tcgtttgggt aggttaccga
     1921 taattcctgc aattgtttgt actgatcggt atacatacaa tcgctcgccg tatttaccac
     1981 aagcactttc tttcctttaa acaccgcaaa atcgatcata ccgccatcga tggaaggaaa
     2041 ttgaaatgca tgtaaagaag atttttcttg cataatcaat atgcacaaat gtaaaggatt
     2101 ttagcaacct ttgtgtgata ttggatgtta gtaggcatat gaagacatca gtaataagaa
     2161 gagcaacttt taatgcagca cacagattgc acaatcctgc atgggatgat gctaccaaca
     2221 gcaaggtttt tggtgcatgt aacaacccga attaccatgg gcacaactac acgttatttg
     2281 taaaaattac aggtgaagtc gatccggtta caggatatgt aatggactta aaagatgtca
     2341 atattctgat caacacatac gtgatagagc ctctggatca tcagaaccta aaccttgacg
     2401 tgcccgattt tcgcaatctg aatcctactg ccgaaaatat tgcacgcatt atctacgaaa
     2461 agctgagaag tgcactggca gcagatcttg agctaaaggt gacactatac gaaaccgaaa
     2521 ataatatcgt agagtatccg ggttgaaatg caatcaacca tttaatttgt gatgcatcaa
     2581 tcctgcgatt ccatcagccc tggttttcat caggtcggca ttttctcctg caaaatattt
     2641 atcaactgta gcgtgaaata attgcagcca gcgatcaaaa tgcgcttgag taagtggcga
     2701 ttttgcatgc agcgcaacat gcggtgtcat agcatttcgt gtatacgatc cggttcccag
     2761 aacggtatat tcccagaaat ctatgataag cggtgtatgt gtttcccaat taacctgcgc
     2821 cacatcatta aaaatatatg ccagcaattc atcgcggcgc acatcattgt aaaattccac
     2881 aacaatcttg gcaacatcgc ttctacttgt aatgtcttgc atcaggctat ggttgttata
     2941 atttcatttg tgccggcaag caatttgtga accggacatt tatctgcaac catcagcagg
     3001 cgatcataaa tagcctgatc ttgcttatgt tcaggcttga tggttctgtt gaagacctgt
     3061 ttgcctgctt ctccttctga aagcgtgact tgcacatcga tattacccaa tgcccagttt
     3121 ttcctttcag catacatgcg caaggtgata gcggtgcagg cggccaggct catacacaac
     3181 aattcccgcg gtgcaggagc actgtctttt ccgcctttat cagcgggttc atcgcaaaac
     3241 accgtgcttc tgccgttgtg catgcttgtt ttatagtttt cggcacctaa tgttgcctca
     3301 atggaaatga aatctgtcat gtagtgttca gtttattgga aattattgaa aatcaaggtt
     3361 tgtaacccac ttaacaagag ttatacggac cttaaccttt tttaactttg caggtcaaat
     3421 gtacacacgt ttcaacttgt ttatataata taattttgtc acccgttttc ttctacgaga
     3481 ccaaacctac ttattatgga tgcagtgata tcaaccgata tccgggagtt gaatgagcga
     3541 atacatcagg aaagtgcttt tattgacctg ctgaggatgg aaacctccaa agcaattgtt
     3601 ggtcagaagt atatgattga ccgcttgtta ctcggattat tatctcaagg acatatttta
     3661 ctggaaggag ttccgggact tgcaaaaact ttagccatta aatcattgtc atcggcggtg
     3721 aatgcaaagt tttcgcgcat acaatttaca cccgacctgt tacctgccga tttgatcggt
     3781 acgatgattt ataatcagaa ggacaacaac ttcagtgtaa agaagggtcc tattttcgct
     3841 aattttattt tggccgacga gatcaaccgt gctccggcaa aagtgcagag tgcattactc
     3901 gaggcgatgc
//
LOCUS       DAOQHY010000250         3865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_14477_length_3865_cov_4.840420, whole genome shotgun sequence.
ACCESSION   DAOQHY010000250 DAOQHY010000000
VERSION     DAOQHY010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14477_length_3865_cov_4.840420"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..915)
                     /locus_tag="PLL28_13985"
     CDS             complement(16..915)
                     /locus_tag="PLL28_13985"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HNF70487.1"
                     /translation="MRKILILLLITGFVSRLEAQELNCAVTVNAEKVTQTDPKIFKTL
                     ESSVFEFMNTRKWTDDAFKPEERIVCQILITVTEELSSDKFRAQASIVSRRPVYGSDY
                     NSTLLNFQDKDFEFQYAEYQALEFNENQFTSNLTAMLAYYAYVIIGLDYDSFAPKGGD
                     KYFLKAQTIVNQASNVDAKGWKSYDGTRNRYWYINNIMDPKFAVMRDAFYQYHRQGLD
                     NLYNDQVKPVSVITKALQTLDNINRTSPNSMIMQLFFSAKSDELLGLYSKATPTEKAK
                     AVSYLTRLDPVNADQYQELLTGK"
     gene            complement(908..2122)
                     /gene="coaBC"
                     /locus_tag="PLL28_13990"
     CDS             complement(908..2122)
                     /gene="coaBC"
                     /locus_tag="PLL28_13990"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNF70488.1"
                     /translation="MSLRGKKVLLGVCGGIAAYKSAYLVRLLTKAGAEVRVMMTPDAA
                     AFVGPLTFATLSKHPVAIDFFDKQTGQWQNHVSTGLWPDVILIAPVTANTIAKLVGGI
                     TDNFLLATTLSSRCPIYLAPAMDLDMWKYQATVDNIAKLKGFGHHIINPVHGELASGL
                     EGTGRMAEPEELLEVMENHFQQVKGPLSGKKVLITAGPTYEAIDPVRFIGNHSTGKMG
                     IALAETCLRLGAEVTLVHGPMQVTCDDADIHSIAVTSAAEMLGACEEAFPACDIFIAA
                     AAVADFTPEAPSAQKIKKGDSQDGSMQLNLRRTTDILGTLSAKKGTRFVVGFALETSN
                     GLEYAKSKLEQKNLDMVVLNTTADRGAGFGHDTNKITLLDKSGEVINFELKSKAAVAQ
                     DIVQHIIRRLHA"
     gene            complement(2125..2469)
                     /locus_tag="PLL28_13995"
     CDS             complement(2125..2469)
                     /locus_tag="PLL28_13995"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNF70489.1"
                     /translation="MSSLKKINTQLSTQIEVQSLKNIANTSGNIYRSIYIVGRRADQI
                     TGMVRGELHSKLEEFASHQDNLEEIHENREQIEISKYYEKLPKSTIMALDEFLNAKLF
                     FHERETEETKTN"
     gene            complement(2447..3301)
                     /gene="bamD"
                     /locus_tag="PLL28_14000"
     CDS             complement(2447..3301)
                     /gene="bamD"
                     /locus_tag="PLL28_14000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNF70490.1"
                     /translation="MAWTRALALTIISISILVFTGCQYDKLLKSDDFDAKYAKAKEYY
                     GKGDYARALPLLEQMLSVKIGTPEEKEVRYYTAYCYYGQADYFSSSSLFRQVFNIFPL
                     SGEAEEMLFMSAKSMYMASPRYNLDQQYTYSAIEAFQYFVDVFPKSALVPEANKYMDE
                     LRGKLETKLINAAELYYNTEHYESAAITFKNVLLEFPDTRDAEEISYKIADSYFQYAG
                     QSVICKKEERYDDAINSGQDFIQRYPNSPKVADAKDLIEKSIDLKEKAITEITTYKIK
                     CDELSEEN"
     gene            3401..>3865
                     /locus_tag="PLL28_14005"
     CDS             3401..>3865
                     /locus_tag="PLL28_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNF70491.1"
                     /translation="MHPQKRKLTLKDMDIFDKLRKDRGPLGKWSDVAHGYMMFPKLEG
                     EIGPRMMFRGKERITWSLNNYLGLANHPEVRKIDAEASAQYGLAYPMGSRMMSGNSDH
                     HEHLEKELSSFVQKEDTFLLNYGYQGIMSVIDAICSRHDVIVYDAESHACIID"
BASE COUNT         1040 a          898 c          810 g         1117 t
ORIGIN      
        1 aggcaagctt gatgcttatt tccccgtaag caactcctga tactgatcgg cattaaccgg
       61 gtctagtctt gtgagatagc tcacggcttt ggctttttct gttggcgtag ccttgctgta
      121 taaccccagc aattcatcac ttttcgcact gaagaataat tgcatgatca tactatttgg
      181 cgaagtgcga ttaatattat caagtgtttg cagagccttt gtaataacag aaacaggctt
      241 cacctgatca ttatataaat tatctaaacc ttgacggtga tattgataaa atgcatcacg
      301 catcacagca aatttcggat ccatgatatt gttgatatac caatacctgt tgcgcgtgcc
      361 atcataactt ttccaaccct tcgcatctac attgctcgcc tgatttacga tagtttgtgc
      421 cttcagaaaa tatttatcgc caccctttgg tgcaaatgaa tcataatcaa gtccaatgat
      481 gacatatgca taataagcca gcattgccgt aagattactt gtaaattgat tttcattaaa
      541 ttccaatgcc tgatattcgg catactgaaa ctcaaaatct ttatcctgaa aattcaacaa
      601 agtactattg taatcgctcc cataaacagg tcggcgagaa acaatactgg cttgtgcgcg
      661 gaatttatcg ctcgataatt cttcagtcac cgttatcaaa atctgacaaa caatacgttc
      721 ctctggttta aatgcgtcat ctgtccactt gcgcgtattc atgaactcaa aaacggagct
      781 ttccagggtt ttaaaaatct tcggatccgt ttgtgttact ttttccgcat tcacggtaac
      841 ggcacagttt aattcctgtg cttcgagtcg cgacacgaaa cctgttatca agaggaggat
      901 taatatctta cgcatgtaat cggcgaataa tatgttgtac aatatcctgc gcaacggcag
      961 ctttactctt caattcaaaa ttaatcacct cgccgctctt atccaacagg gtgatcttat
     1021 tggtatcatg tccaaagcct gcaccgcggt ctgctgtagt attcaatact accatatcga
     1081 ggtttttctg ttcaagcttc gatttggcat attctagccc attgctcgtt tctaacgcaa
     1141 atccaaccac aaaacgcgtg cccttcttag cactgagggt tcccaggatg tcggttgtgc
     1201 gcctcaaatt gagctgcatc gagccatcct gactatcacc cttttttatt ttttgtgccg
     1261 atggggcctc cggtgtaaaa tcagctactg ctgcagccgc tatgaagata tcgcaggcag
     1321 gaaaggcttc ttcgcaagcc ccgagcattt ctgccgctga ggttactgct atactatgaa
     1381 tatcggcatc atcgcatgta acttgcattg ggccgtgcac caaggtcact tcagcaccca
     1441 atcgcagaca tgtttcagct aaagcaatac ccatttttcc ggttgaatga tttccaatga
     1501 aacgaacagg atctatagct tcatacgtgg gacctgcggt tatcagcact tttttacctg
     1561 aaagcggacc ctttacctgc tgaaaatgat tttccatcac ttcgagcaat tcttcaggtt
     1621 cagccatacg tccggtaccc tctaatccgc tggctaattc gccatgaacg gggttaatga
     1681 tgtgatgtcc gaaacctttt agtttggcga tattgtctac cgtagcctga tacttccaca
     1741 tatccaggtc cattgccgga gccaggtaaa ttggacatct ggacgatagg gtagtggcaa
     1801 gaagaaagtt atctgtaata cctcctacca gtttagcaat cgtattggca gttacagggg
     1861 cgatcaggat gacatcaggc caaagcccgg tgctcacatg attttgccat tgccctgttt
     1921 gtttatcaaa aaagtcgatg gcaacggggt gtttacttaa agtagcaaag gtcagggggc
     1981 ctacaaatgc ggctgcatcg ggtgtcatca tcacacgaac ctcggcaccg gcttttgtga
     2041 gcaggcggac aaggtaggcg cttttatatg cagcgatccc gccacatacc ccaagtagga
     2101 ccttcttgcc ccgtagcgac atgattagtt ggttttagtt tcctcagtct cgcgttcgtg
     2161 gaagaacaac ttagcgttga ggaattcatc caatgccata atggtagatt tcggcagctt
     2221 ttcgtagtat ttgctgatct cgatctgttc tctgttttcg tggatctcct caaggttatc
     2281 ctgatgtgaa gcaaattcct caagcttgct gtgcaattca ccgcgaacca tacctgtgat
     2341 ctgatcggca cgacgtccta caatatagat agaacggtat atgttaccgc ttgtgttagc
     2401 aatgtttttg agactttgca cttcaatctg ggtagataat tgcgtattaa ttttcttcag
     2461 agagctcatc gcatttgatt ttatatgtgg taatttctgt aattgctttt tctttaaggt
     2521 caatcgattt ttcaatcaaa tctttggcgt cagcgacttt cgggctattt ggataacgct
     2581 gaatgaaatc ctgaccacta ttgattgcat catcgtaacg ttcctctttt ttgcagatca
     2641 cactttgacc ggcatactga aagtagctat cggcaatctt atagctgatt tcctcggcat
     2701 cacgggtatc cgggaattcg aggagcacat ttttaaaagt gatggccgca gactcataat
     2761 gctcggtatt ataataaagt tcggcagcgt tgataagctt ggtctccagc ttgccacgaa
     2821 gctcatccat gtacttattc gcctcaggca cgagcgcact tttcgggaat acgtctacaa
     2881 aatactgaaa tgcctcaatc gcactataag tatactgctg atctaaatta tacctcggcg
     2941 aagccatata catcgatttg gcactcataa acaacatctc ctcagcctca ccgcttaatg
     3001 ggaagatatt gaaaacctgg cggaaaaggc ttgatgaact gaagtaatca gcctgtccgt
     3061 agtagcaata cgcagtgtaa taacgcacct ctttttcctc cggggtgcca atcttcaccg
     3121 acagcatctg ttccaaaagc ggcagggcac gggcataatc gcctttgcca tagtattcct
     3181 tagctttggc atacttagcg tcaaaatcat cacttttcaa cagcttatca tattgacagc
     3241 cggtgaaaac taatatagaa atgcttataa ttgtgagtgc aagagcccta gtccacgcca
     3301 taagaggtgc aaagttaatc attttcgcct aaacgatgga aggcttaatt tattgaaaag
     3361 gaggcgttaa attttttatc cccatattaa cgcaatatct ttgcacccgc aaaaaagaaa
     3421 acttacacta aaggatatgg atatctttga caagttaaga aaggaccgcg gtccactggg
     3481 caaatggagt gatgtagcac acgggtatat gatgttccct aaactggaag gggaaatcgg
     3541 ccctcgcatg atgttccgcg ggaaagagcg gatcacatgg agtctgaaca attacctcgg
     3601 actggcaaac cacccggaag tgcgtaagat cgatgctgaa gcctcggctc agtatggtct
     3661 cgcttatcca atgggaagcc gcatgatgag cggtaactcc gatcatcacg agcacctcga
     3721 aaaagaactg agcagctttg ttcagaaaga agataccttc ctgctcaact acggctatca
     3781 aggtatcatg agcgtgatcg acgctatctg cagtcgccac gacgttatcg tttacgatgc
     3841 cgaaagccat gcatgtatta tcgac
//
LOCUS       DAOQHY010000251         3861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_14503_length_3861_cov_4.028376, whole genome shotgun sequence.
ACCESSION   DAOQHY010000251 DAOQHY010000000
VERSION     DAOQHY010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14503_length_3861_cov_4.028376"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PLL28_14010"
     CDS             <1..732
                     /locus_tag="PLL28_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70492.1"
                     /translation="GSLPDMRAVAAKPWFENTLLLGVMFISVFYVTALTVKHSGLAVA
                     SIANKLSLIIPVVAAYFLYDDHFSFLKIAGILLAMIAVVFTVYKPDTETHKHDFSYYF
                     LPLVVLIGGGAIDTFTKWNQENLLAPEDFNVYLVMVFGTAAGFGWLIVIYRRLRFSEK
                     ISLRTIGHGLLLGIPNYGTMYFLLAVLNQPGWSSSVVFPVNNIAVVAVSCLVAMVLFK
                     EKLSKLNLTGIALSMLAIFLIMQSM"
     gene            734..1147
                     /locus_tag="PLL28_14015"
     CDS             734..1147
                     /locus_tag="PLL28_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70493.1"
                     /translation="MLNELDALNLIRKKRYPYVLGGTLCVLTCLALTIEFVAGASHKY
                     LFTTLGTELLFYMLVNAISCLLVPDMWIHVKKTIPLYLVNLAITGSLIYLFIDGAVRD
                     HRAFFPALGALVFCFFASMVLIMVIRMVASFLKQE"
     gene            1158..1646
                     /locus_tag="PLL28_14020"
     CDS             1158..1646
                     /locus_tag="PLL28_14020"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HNF70494.1"
                     /translation="MPVNIIVTGGTFDKEYDEIKGQLYFKETHLPEILRLGRCTVPVH
                     IDTLMMIDSLHMTDDDRARIVSHCAECSNKKIIITHGTDTMVETAKVLAAARLEKTIV
                     LTGAMIPYKFGSSDGLFNMGCALSYVQTLPPGVYITMNGKCFTWDNVRKNREEGLFEE
                     LN"
     gene            complement(1736..2230)
                     /locus_tag="PLL28_14025"
     CDS             complement(1736..2230)
                     /locus_tag="PLL28_14025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNF70495.1"
                     /translation="MKKEVVFIVDDEPMHAQMMEDHLRSKYPQFEIYKYSTGEECNDN
                     MDKNPGIVILDFHLDAVNKSARNGLEVLEHLKKNYECDVIMISGQDKIDVAVECMRAG
                     AYDYVVKNETSFHRTETAIERLFKHRSVVHELAKYKRNNRILTYGIIGIILLTVVLTA
                     AGVL"
     gene            complement(2259..2615)
                     /locus_tag="PLL28_14030"
     CDS             complement(2259..2615)
                     /locus_tag="PLL28_14030"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNF70496.1"
                     /translation="MESYNRINLAFLETFTNNDKAKMIKYINMFLQLAPASIETMKQQ
                     HAAGDWNNLKTTAHSLKPQLAYMGIESLRENVIRIEEYAGEQKNPDSIAQLISLVDTG
                     CTEAFAELQDVIGKIA"
     gene            complement(2627..>3861)
                     /locus_tag="PLL28_14035"
     CDS             complement(2627..>3861)
                     /locus_tag="PLL28_14035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF70497.1"
                     /translation="RMRDAAYDQRVLREKVRDKTRELEMEKERAEQSERVKEQFLANM
                     SHEIRTPLNAILGMTRLLLDKEPRNDQFKYLNSIKHASDNLLVIINDILDLSKIEAGK
                     INFERIPFRLRNELQAVVDTVKVNADDKGLALKLDIADAVPDSLSGDPYRLNQILLNL
                     VGNAIKFTEKGSVTIKAELLSSDNNKVDMQFSVIDTGIGIAKDKLDYIFDMFTQESSS
                     TTRKFGGTGLGLAICKKLVELQGGEIAVSSTVGTGSQFKFRISYDKAEESADTGEEKS
                     TGTQRNLSNLKILLAEDNEFNQMVAVDTLEGAIPGAKVDVAQNGKLAVEMVIANDYDL
                     VLMDIQMPEMDGHEATRTIRSNTDARVNSIPIIAMTASVIKAEVDKCFESGMNEFVGK
                     PFNVEELLDKIAKTISPL"
BASE COUNT          905 a          789 c          921 g         1246 t
ORIGIN      
        1 ggctcattgc cggatatgcg cgctgttgcg gcaaaaccct ggttcgaaaa taccctgttg
       61 ctcggtgtga tgtttatttc ggtgttttat gtaacggcat taacggtgaa gcacagtgga
      121 cttgcagtgg cttccattgc gaataaattg tcgctgatca ttccggtggt ggctgcgtat
      181 tttttatatg acgatcattt ttctttcctg aagattgcgg gcatattgtt ggcgatgatc
      241 gcggtagtat ttacggtgta taaaccggat acggaaacgc acaagcatga tttcagttat
      301 tattttttac cgctggttgt gttgatcggc gggggagcca tagatacatt tacgaaatgg
      361 aatcaggaaa atttactcgc gcctgaagat tttaatgtgt atctggtgat ggtatttggt
      421 acggcagcag gtttcggctg gctgatcgtg atctatcgcc ggctcaggtt ttcagaaaaa
      481 ataagtctgc gcaccatagg ccatggctta ttgttaggca tacccaacta tggaacgatg
      541 tattttcttt tggcagtact caaccaaccc ggttggagca gttcggtggt ttttccggtc
      601 aacaatatcg cagttgttgc tgtttcctgt ctggtggcaa tggtattgtt taaagagaag
      661 ctcagtaaat taaatctgac cggcattgca ttgtcgatgt tggcgatatt tctcatcatg
      721 caaagtatgt agtatgctta acgaattgga tgccctgaac ctgatccgca aaaaacgtta
      781 tccctacgta ttggggggca ctttatgtgt gcttacttgt ctggcattaa cgatagaatt
      841 tgttgccggc gcttcgcata aatatttatt caccacctta ggtacggaac tgttatttta
      901 tatgttggtg aatgccatca gttgtttgtt agttcctgat atgtggatac acgtaaaaaa
      961 gaccatccca ttatacctgg tcaatctggc tatcaccggc agtttgatct atttgttcat
     1021 tgatggtgcg gtgcgcgatc accgggcgtt tttcccggcc cttggcgcat tggttttttg
     1081 tttctttgca tccatggtat tgatcatggt gatcagaatg gtagcttcat ttctcaaaca
     1141 ggaataaaca gattttcatg cctgtaaata ttattgtaac cggcggaacc tttgacaagg
     1201 aatacgatga gatcaaaggt cagttgtatt ttaaggaaac ccacctgcct gaaatattgc
     1261 gcttgggtag atgtacggtg cctgtgcata ttgacaccct gatgatgatt gacagtttgc
     1321 acatgacgga tgatgaccgt gcacgtattg tttcgcattg cgcggaatgt tcgaataaaa
     1381 agatcatcat tacacatggt acggatacca tggtagaaac tgcaaaagtg ttggctgcag
     1441 ccagacttga aaaaacgatc gtattaacag gggcaatgat cccatataaa tttggttcca
     1501 gcgacggctt attcaatatg ggatgtgcgc tatcctatgt gcaaacattg ccacccggtg
     1561 tgtacatcac catgaatggt aaatgtttta cctgggataa tgtgcggaag aacagagagg
     1621 aaggtttgtt tgaagaattg aattaataca cacctgcaaa aaagaaaacg ggaagcaact
     1681 taccggatga tccgatgggt tgcttcccgt atatgttttg ccactgttga ggcgatcaca
     1741 acacgcctgc agcagttaat actactgtca gtaaaatgat accgatgatc ccatacgtga
     1801 ggatgcggtt attgcgttta tattttgcca gttcgtgcac aacagaacga tgtttgaaca
     1861 ggcgttcgat agcagtttct gtgcggtgga agctggtttc atttttcaca acgtagtcgt
     1921 aagcacctgc acgcatacac tcaacggcta catcgatctt gtcctgaccg gagatcatga
     1981 tgacatcgca ttcgtaattt tttttcaggt gttcgagtac ttccaatcca ttacgcgcgc
     2041 ttttatttac ggcatccagg tgaaagtcga ggataacgat gcccggattt ttgtccatgt
     2101 tgtcgttaca ttcttcaccg gtgctgtatt tatagatttc gaactgtggg tatttgctac
     2161 gcagatgatc ttccatcatt tgggcgtgca taggttcatc gtctacaata aatactactt
     2221 cttttttcat atagctgatt gggtttctga tgacaatatt aagcaatttt tcctatcaca
     2281 tcctgcagtt cggcgaaagc ctcagtgcaa cctgtatcta ccaggctgat cagttgtgca
     2341 atggaatcgg ggtttttttg ttcgccggcg tattcttcga tacggatcac gttttcgcgt
     2401 agcgactcga tacccatata ggccagttgt ggctttaggg agtgtgcggt ggttttcaga
     2461 ttgttccagt cgccggcagc atgttgttgc ttcatggttt caatactggc cggagcgagt
     2521 tggaggaaca tgttgatata cttgatcatt ttggccttat cattattggt aaaggtttcc
     2581 aggaaggcca gattaatgcg gttgtacgac tccatgatgt gcgaatttat aacggagaaa
     2641 tggtttttgc tattttgtct agtaattctt ctacattgaa gggtttgccc acaaattcgt
     2701 tcatcccgct ttcgaagcat ttgtcgactt ccgccttgat cacagaggcg gtcatggcga
     2761 tgatgggaat ggagttcacc cttgcgtctg tattagagcg gatggtgcgc gtagcttcgt
     2821 gaccatccat ttccggcatc tggatgtcca tgagcacgag atcgtagtcg tttgcaatca
     2881 ccatttccac tgcgagtttg ccattttggg caacgtcaac ttttgctccg ggaatggcgc
     2941 cttctaaagt gtctacggct accatttggt tgaattcatt gtcttcagcc agcaatattt
     3001 tcaggttgct caaattgcgc tgtgttccag tagatttttc ttcgccggtg tcagcgcttt
     3061 cttcggcttt gtcgtaacta atgcggaatt taaattgcga tcctgttcca acagtgctgc
     3121 ttacggctat ttcgccacct tgtaattcga ccagtttttt gcagatagca agtccgagtc
     3181 ctgtgccacc gaattttctt gtggtgctgc tcgattcctg tgtgaacata tcgaagatat
     3241 aatccaattt atctttggcg atgcctattc cggtatcaat tacactgaat tgcatatcga
     3301 ctttattatt atctgaagac aacaattctg ctttgatggt aacgctgcct ttttctgtga
     3361 acttgatggc attgcctacc agatttaata atatttggtt gaggcgatac ggatctccgc
     3421 ttagtgaatc cggcactgca tcagctatat ccaatttcaa tgccaatcct ttatcatcgg
     3481 catttacttt cacggtatcg accacggcct ggagttcgtt gcggaggcgg aatggtatgc
     3541 gttcgaaatt tatttttccg gcttcgattt ttgagaggtc gaggatatcg ttgatgatga
     3601 cgagcagatt atcggaagca tgttttatgc tattgagata tttaaattga tcgttgcgtg
     3661 gttctttatc taggagcaga cgagtcatcc ccaaaatagc attcagcggt gttctgattt
     3721 cgtgactcat attggcgagg aactgttctt tcacgcgctc agattgttcg gcgcgttctt
     3781 tttccatttc cagttctcgt gttttatcgc gcactttttc gcgcagcaca cgctgatcgt
     3841 atgcggcatc gcgcatgcgt t
//
LOCUS       DAOQHY010000252         3796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_14886_length_3796_cov_4.458968, whole genome shotgun sequence.
ACCESSION   DAOQHY010000252 DAOQHY010000000
VERSION     DAOQHY010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14886_length_3796_cov_4.458968"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PLL28_14040"
     CDS             <1..427
                     /locus_tag="PLL28_14040"
                     /inference="COORDINATES: protein motif:HMM:NF019122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70498.1"
                     /translation="ALGSGMVSGLDLGENARALLITKGWAEIIKLSEVLGADVKSFLG
                     LAGIGDIIATCSSPSSRNFSVGFRLAKGEKIKAIQTSMEEVAEGINTIRIANGLANFH
                     GIHCPIIKTLYQGIFEDLNVQTGIEYLMRSRRSMDVEFL"
     gene            complement(431..934)
                     /locus_tag="PLL28_14045"
     CDS             complement(431..934)
                     /locus_tag="PLL28_14045"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNF70499.1"
                     /translation="MRKLSVVLGFVALSLSVVGCKNDESEPPVKPHHLGAGQPQFQKQ
                     GELSFLNVEGTVLSKINIEIADTPDRQQKGLMNRSFMSNDNGMLFIFDVERPQSFWMR
                     NTIIPLDIIYVNSKMQIVSIAKNTTPYSEESIPSRGPAIYVVEVNGGYCDQYSIQPGC
                     LIQFTKL"
     gene            1094..1882
                     /locus_tag="PLL28_14050"
     CDS             1094..1882
                     /locus_tag="PLL28_14050"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF-related protein"
                     /protein_id="HNF70500.1"
                     /translation="MQIVDNPDQKTPPASEPKEPVRQKRFYLTGTALLVLGIIYFLQK
                     ATNLETPLDFISWELILIITGVYIGEKHNFTGVSWLICIAIGAWFLLDSYIPTVNLRY
                     YFGPIILIAIGAYLLFGDRRIRYRRNKYRNYGRPYTKEQGFAADNSASDDYIDVVSVF
                     GGVSKQMFTKQFKGGEATSIFGGTEINLMQADFEGKAVLELTQIFGGCTLIIPAHWDL
                     KTEVVSIFGGVEDNRRGNTTLIDHKRVLLLKGTSIFGGIEIKSY"
     gene            1894..2946
                     /locus_tag="PLL28_14055"
     CDS             1894..2946
                     /locus_tag="PLL28_14055"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNF70501.1"
                     /translation="MSTNRIPVNKYATPFLLSWFIWSCVYAGLLKYLGANLSIAVIDS
                     LVFNGALLLCAILQVVVLQYYFPKQNKTSYVLVVSLIVSTIVTAFSTFIMSQFPEQQF
                     SITTQQDVYISYTNIAIPLRFSLAFLLAACIAMINILFFTLQEEKSTVQRKIEAEQLA
                     REAELFQLRSQLQPHFLFNSLNSISALAGSKPEEARRMIQQLSDFLRGTIKKDNSQLI
                     TLEEEIKHLSLYLDIEKVRFGHRLQTNIDCPDDCYKLKIPSLLLQPVLENAIKFGLYD
                     TLEEVSIDIRARYDEPYLKVEITNPFDKDTQPAKGTGFGLQAVSRRLYLLYARTDLLQ
                     TSSEQHIFKTVITIPQ"
     gene            2943..3683
                     /locus_tag="PLL28_14060"
     CDS             2943..3683
                     /locus_tag="PLL28_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HNF70502.1"
                     /translation="MNPIKVIIIDDEPLARSIVAEYIASHSQLTIVAECGDGFEGVKA
                     IAQHQPDLIFLDVQMPKINGFEMLELLEDKPDVIFTTAFDEYAIKAFDIHAIDYLLKP
                     FNKERFDAAISRWQSMRGTKTATALSTEEIIPAEQQPQRVVIKLNNKIKIIPFSDIHF
                     IEAAEDYVMIHTGEGRYMKHKTMTFFEKALPANQFVRCHRSYIVDVQQINRLEAYEKD
                     SQIAILKSGAKVPVSKSGYIRLKQVLGV"
BASE COUNT         1148 a          756 c          806 g         1086 t
ORIGIN      
        1 cgcattgggt tcggggatgg tcagcggcct ggatctcggc gaaaatgcac gtgctctgct
       61 cattacaaaa ggctgggccg aaatcattaa gctgagtgaa gtattgggtg ccgatgtaaa
      121 atcattcctc ggacttgcgg gaatcggtga tattattgct acttgctcat caccaagcag
      181 tcgaaatttc agcgttggat ttcgtttggc aaaaggcgaa aaaataaaag ctattcaaac
      241 cagcatggaa gaggttgcag aaggtattaa taccatacgc attgccaatg gcctcgcaaa
      301 tttccatggc atacactgcc ctatcataaa aacattatac cagggaattt ttgaagacct
      361 caatgtacaa acaggcatag aatacctgat gcgcagcaga agaagtatgg atgtggaatt
      421 tctttaatcc tcagagctta gtaaactgaa tcaggcatcc gggttgaata ctatattgat
      481 cgcaataacc accattcacc tccaccacat aaattgcagg tccgcgactt ggaattgatt
      541 cctcagaata cggtgtcgta ttttttgcga tggacacaat ttgcattttg ctgtttacat
      601 aaataatatc caaagggatg atcgtatttc tcatccaaaa tgactgtgga cgttcaacat
      661 caaaaatgaa cagcatgcca ttatcgttcg acataaaaga tctgttcatt agtccttttt
      721 gttgacgatc aggcgtatca gcaatttcga tattgatttt tgacaataca gtaccttcca
      781 cattcaaaaa ggatagttct ccctgctttt gaaattgagg ctgtccggct ccaagatgat
      841 gcggttttac cggtggttca gattcatcgt tcttacaccc aacaacactt aatgacaagg
      901 caacaaagcc caaaacaacg gatagtttac gcataaacgg taattacgga gcaaagatag
      961 acgaatttcc atgttttgct ccccttcatt cgccgccaaa attgtgccat tggtcgaact
     1021 tttggctcac cagatgcctt tgtgcttgct agaaagcttc agacggggat acatttgctt
     1081 aaaattgatt gatatgcaga tcgttgacaa tcccgaccag aagacccctc ctgcttccga
     1141 accaaaagaa cccgttcgcc aaaaaaggtt ttacctcaca ggaacggcat tgctggttct
     1201 aggtattatt tacttcctcc aaaaagctac caaccttgaa acaccacttg atttcatcag
     1261 ctgggaattg atattgatca tcaccggggt ttacattggc gagaaacaca atttcacagg
     1321 tgtgtcatgg ttgatttgca tagccattgg tgcttggttt ttgctcgatt cctatatacc
     1381 aacagtaaat ctccgatatt acttcggacc catcattcta attgccatcg gtgcgtattt
     1441 attattcggc gatagaagaa ttcgctaccg tcgcaataaa tatcgcaatt acggtcgccc
     1501 ctacacgaag gaacaaggat ttgccgctga caacagtgcc tctgatgatt atattgatgt
     1561 tgtttcagta ttcggcggtg taagcaaaca gatgtttacc aaacaattca aaggtggcga
     1621 agcgacaagt attttcggcg gcacagaaat taatttgatg caggccgatt tcgaagggaa
     1681 ggcagtattg gaactcactc aaattttcgg cggatgcaca ctgataatcc ctgctcactg
     1741 ggatttaaaa actgaggtgg tttctatatt cggtggtgtt gaagacaatc gtcgcggcaa
     1801 cactacgctt atagatcata agcgcgtatt attattgaaa ggaacatcta tttttggcgg
     1861 gatagagatt aaaagttact gatactaact ttcatgtcga caaatcgcat acctgtaaac
     1921 aagtacgcaa ctcctttttt attgagttgg tttatttggt catgcgttta tgcgggtttg
     1981 ttaaaatatc tgggcgcaaa tctttcaata gccgtcatcg acagcttagt atttaatggt
     2041 gccttattgt tatgtgcaat tctacaagtg gtagtgctgc agtattactt cccgaagcaa
     2101 aataaaacga gttatgtatt agttgtttca cttattgtca gcacaattgt aactgcattc
     2161 agcacattta ttatgtcgca gtttcctgaa cagcagtttt cgataacaac gcaacaagat
     2221 gtatatatca gctataccaa tatcgccatt ccgctgcgat tcagtctggc atttttattg
     2281 gctgcctgta ttgccatgat taatattttg ttctttacgc tgcaagaaga aaaatcgacc
     2341 gtgcaaagaa aaattgaagc tgaacagctt gcccgtgagg cggaattatt ccaattgcgc
     2401 agtcagttgc aaccgcattt cttatttaac agcttgaatt ccatcagtgc attagcaggc
     2461 agcaaacctg aagaagctcg acgtatgata caacagctat cagatttttt gcgaggcaca
     2521 ataaaaaaag ataacagtca gctcatcaca cttgaagaag aaataaaaca cctttcacta
     2581 tatctcgata tcgagaaagt gcggttcgga catcgcttgc agacgaatat tgattgccct
     2641 gatgattgtt ataaattaaa aataccatca ttattattgc aacctgttct tgaaaatgca
     2701 ataaaattcg gattgtatga tacgcttgaa gaagtaagta tcgacatacg agcaagatat
     2761 gatgagccat atcttaaggt ggagattacc aatccttttg ataaggacac acagcctgca
     2821 aaagggacgg ggtttggttt acaggctgtg agcagaagat tatatttgct gtatgccagg
     2881 acagacttac tccaaacaag ctctgagcaa catattttta aaactgtaat taccattccg
     2941 caatgaatcc gataaaagtc attattatcg acgacgagcc cttagcacgc agtatcgtag
     3001 cagaatatat tgcttcgcat agtcagttaa ccatagttgc agaatgtggc gatggatttg
     3061 agggtgtgaa ggcaattgct cagcatcaac cggatttgat tttccttgat gtgcagatgc
     3121 caaaaataaa tggatttgaa atgctcgagc ttctggagga caaaccggat gttattttca
     3181 caacggcatt tgatgaatat gcgataaaag catttgatat tcatgccatc gattatttgc
     3241 tcaagccatt taataaagag cgatttgatg ctgcaatatc gagatggcag tcgatgcgtg
     3301 gaacgaaaac agcaactgca ctttcaacgg aagaaataat tcccgcagag caacagcctc
     3361 agcgagttgt tatcaagctc aacaataaaa tcaagatcat accattttcc gacattcatt
     3421 ttattgaagc tgctgaggac tatgtgatga tacataccgg tgaaggccgg tatatgaagc
     3481 ataaaacgat gacctttttc gaaaaggcat tgcctgcaaa tcagtttgtg cgttgtcata
     3541 ggagttatat tgttgatgtg caacaaatca atcgactgga ggcctatgaa aaggattcac
     3601 aaatcgcgat tctaaaatcc ggcgctaaag tacctgtgag taaaagtggg tatataaggc
     3661 ttaagcaggt tttgggagtg tgagggaatc ccgcttgggc gggatgtcgc tgcgctccat
     3721 gtgggaatgt atccgaaatt tgtatcaggt atcaagtatc aggtatcaag tatcaggtat
     3781 caagtatcag gtatca
//
LOCUS       DAOQHY010000253         3790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_14920_length_3790_cov_6.891031, whole genome shotgun sequence.
ACCESSION   DAOQHY010000253 DAOQHY010000000
VERSION     DAOQHY010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14920_length_3790_cov_6.891031"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..693)
                     /locus_tag="PLL28_14065"
     CDS             complement(88..693)
                     /locus_tag="PLL28_14065"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNF70503.1"
                     /translation="MLQAYHQTELLEAGCDEAGRGCLAGPVVAAAVILPLDFYHPLLN
                     DSKQLSESTRNTLAPVIRENAIAWAIGICDQTEIDKLNILRASFAAMHKAINQLSVRP
                     QLLLIDGNRFHKYPGIPHLTMIKGDARFASIAAASILAKTVRDEMMQQLHVEYPVYNW
                     SSNKGYPTSEHRDAIRIHGDTKYHRKTFRLLPEQERELFGG"
     gene            784..1674
                     /locus_tag="PLL28_14070"
     CDS             784..1674
                     /locus_tag="PLL28_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF70504.1"
                     /translation="MEPVLVTVQLKKHFGPIKAVNGLDLAVQKGAVYGILGPNGSGKT
                     TTLGMLLGIVHPSGGDYSWFGNGQKDENRKRVGALLEQPNFYPYLSGEQNLKVVARIK
                     GVNPSEIDTVLAQVNLLESKKRQARTYSLGMRQRLALASALLGNPDVLVLDEPTNGLD
                     PQGIAEVRDFIRQQSAQGRTIILASHILDEVEKVCSHVSVIKKGNLLAQGTVNDLLKG
                     EDQVEIGAKDINALSNALEGYPGIRRLEKTNDLIVLHVNEAFDTAALNKFFSDKQIHL
                     SHLVGRKKRLEEQFLELTAE"
     gene            1676..2455
                     /locus_tag="PLL28_14075"
     CDS             1676..2455
                     /locus_tag="PLL28_14075"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNF70505.1"
                     /translation="MLRLLKIELHKLLPYRTFKILFIVYLLLMVLWLFAGKGLLSGGP
                     MTPSQILGFPNIWNYYALYACYFNIILAILIIFVTCNEYTYRTLRQNVIDGMQRNETV
                     LGKIYLIILLSIISTLVLSISGVIGGLIFSNADDKSKIFDHSGFIFAYLIQSLGMMSM
                     GYFFGTLFKRTGLAVIIFLIFLFPIDVILREGILPDGSGDYMPVATYFLKMVPWPVSH
                     MMSRGEEPTPGAPTLLAMSVGAAYAVIFTALGWLITKKRDL"
     gene            complement(3050..>3790)
                     /locus_tag="PLL28_14080"
     CDS             complement(3050..>3790)
                     /locus_tag="PLL28_14080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70506.1"
                     /translation="PLNPARMEAGDVPLYLDPVDDYLHIGVNEIPIMLGDDVTTVDAI
                     YGIAFTISVDGENIDASALKINWDDNWMGNIGESLSFSKTFGDTKQVVGGFVRKTRTN
                     VGGDGQIGTLQIVVVDNISGKMDLSEVEISFTDVSATLLNRDVITVAPETLTISAEDN
                     ASVSTNDLNSLYVYPNPVTDNMYFNFDENLTPEQIRIISITGDELLVLNNIGDIAKGI
                     NVSKLAAGTYIVEYNFGSNIATEQFVKQ"
BASE COUNT         1094 a          775 c          788 g         1133 t
ORIGIN      
        1 cctgatactt gatacctgat acttgatacc tgatacttga tacctgatac ctgatacctg
       61 atacctgcta accatacccg aaacccacta ccctccaaac aactctcgtt cctgttcggg
      121 taatagtctg aatgtttttc tatgatattt tgtatcgcca tgtattctta ttgcgtcgcg
      181 atgttctgag gtgggatagc ctttgttaga cgaccaatta tataccggat attctacatg
      241 caattgctgc atcatttcgt cgcgaacggt ttttgccaat atacttgctg cggcaatact
      301 cgcaaatctg gcatcacctt tgatcatggt gaggtgcgga atgcccggat atttgtgaaa
      361 gcggttgcca tcaattaata ataattgtgg tcgcacggat aattgattga ttgctttatg
      421 catagcagca aaacttgcgc gaagaatatt gagtttatca atttcagttt gatcgcaaat
      481 accaattgcc caagcgatgg cgttttcacg aataacaggt gcaagcgtat tccgtgttga
      541 ttctgaaagc tgtttcgagt cgttgagcag cggatgataa aaatcaagcg gaagaataac
      601 ggcagcagca accaccggac cggcaaggca accgcgacct gcttcatcgc agccggcctc
      661 caataattcg gtttgatggt atgcttgtaa cattaaatat tattcatgcg ttatacatat
      721 acacgctttc ggtatgataa ttgccgaagt ggtgattaca tttacactca aacttaacac
      781 aatttggaac cagtattagt gaccgttcag ttgaaaaagc acttcggtcc gattaaagcg
      841 gtcaacggac tcgaccttgc ggtgcaaaaa ggtgctgttt atggtatcct agggccaaat
      901 ggaagtggca aaaccactac cttaggtatg ttgcttggca tagtgcatcc ttcgggtggc
      961 gattattcat ggtttggcaa tggccaaaaa gatgaaaacc ggaaacgcgt cggcgccttg
     1021 ttagagcaac cgaattttta tccctacctc agtggcgaac agaatttgaa agttgttgcc
     1081 cgcattaaag gcgttaatcc gtccgaaatc gatactgttc tggcacaggt taatttgctc
     1141 gaaagcaaaa aaaggcaggc gcgcacttat tcattgggaa tgcgtcagcg actggcactg
     1201 gcatcagcct tgttaggcaa tccggatgta cttgttctag atgaaccaac aaatggtctc
     1261 gaccctcagg gtattgccga agtacgtgat tttattcgcc aacaaagtgc acagggacgc
     1321 accattattc ttgccagtca cattcttgat gaggtagaaa aagtatgtag ccacgtaagc
     1381 gtcatcaaaa aaggaaatct gcttgcacaa ggcaccgtaa atgatttatt gaaaggagaa
     1441 gatcaggttg agattggtgc gaaagacata aatgccttga gcaatgcact tgaaggatat
     1501 cccggcatac gccgtcttga aaaaacaaat gatttgattg tattacacgt taatgaagca
     1561 tttgatactg cggcattaaa taaattcttt tccgataaac aaatacatct cagtcatctg
     1621 gtaggacgca aaaaacgact tgaagaacaa ttccttgaac taactgctga ataacatgct
     1681 tcgattatta aaaatagaat tgcataagct gctgccttat cgcacattca aaatactttt
     1741 tattgtgtat ttattgttga tggtgctctg gctgtttgcc gggaaaggtc tgctatcagg
     1801 aggtcccatg acaccatctc aaattctggg atttccgaat atctggaatt attacgcact
     1861 ttacgcctgc tatttcaata tcattttagc tatactgatc atttttgtaa catgcaacga
     1921 gtatacctat cgcacattgc gtcagaatgt aattgatggc atgcagcgca atgaaacagt
     1981 gcttggaaaa atttatctca tcattttatt aagcatcatc agtactttgg tattaagcat
     2041 tagtggtgta attggcggac taattttttc caatgctgat gataaatcta aaattttcga
     2101 tcacagtggt ttcatcttcg cctatcttat tcaatcgctt ggcatgatga gcatggggta
     2161 ttttttcgga acacttttca aacgcacagg attagcggtt atcatttttc tcattttcct
     2221 ttttccaatt gatgtgattt tacgcgaagg cattttaccc gatggctccg gcgattatat
     2281 gcctgttgcc acctatttcc tgaaaatggt accatggcct gtttcacata tgatgagtcg
     2341 aggagaagaa cctacacccg gcgcacctac tttattggca atgagtgtag gcgcagcata
     2401 tgctgttatc tttacggcat tgggatggtt gatcacgaag aaaagagatt tgtaatagtg
     2461 ccttgaaagg cctcagaaaa aggcggagtg agccggtttg caccgattct ctccgccttt
     2521 ctgatttcag ccttctctct tgctttccat acaggggaca aaaaaaaccg catcgaggag
     2581 ctgatgcggt ttagcgactc taaatatgag aactaaaaaa atttggactt aacccttgtc
     2641 agtcgttacc ctgacgaaga caaaaatgca tcagtattga aaagtatcct aagacaaaaa
     2701 tttgagttta ttgtactcgt ttttaggcct aaaaatacat aatacactga tttacagcca
     2761 tttagacaat ttttttacgc ctgttttgtg gaatttcatg caattatcgg atgacattta
     2821 tccgataaat caggctgatt ttcctcataa atactcaaaa gtggccaaaa actcacccga
     2881 taaatgcaaa aatgggggct tatcggaaag catttgactg aaacctatta aggcaataag
     2941 attgctttag gtcattttga atggtatgct cccatacgag acaaaaaaac ccgcatcgag
     3001 gagagatgcg ggttgaggag cgaaacagat gattgatcag ccggatcttt tactgtttta
     3061 caaattgctc agtggcaatg ttgctgccaa aattatattc aacgatgtaa gttcctgctg
     3121 cgagtttact tacattgatt ccttttgcga tatcgccgat attattcaat accagtagtt
     3181 catcgccggt gatactgatt atacgtattt gttccggagt cagattttca tcgaaattaa
     3241 aatacatgtt atcggtaaca ggatttggat atacgtataa tgaatttaaa tcgttagtgc
     3301 ttacacttgc gttatcctcc gcgctgatgg taagtgtttc aggagcaacg gtgattacat
     3361 cgcggttgag gagtgtagca gatacatcgg taaagctgat ttcaacttca gagagatcca
     3421 ttttaccgct gatattatct accacaacaa tttgcaatgt tccgatctga ccatcgccgc
     3481 caacgttagt tctcgttttg cgaacgaatc cgcccacaac ctgtttggta tcgccgaatg
     3541 ttttgctgaa gctgagtgat tcaccgatat ttcccatcca gttatcatcc caatttattt
     3601 tcaatgcaga agcatcgata ttttctccat caacactgat tgtaaatgca ataccataaa
     3661 tggcatctac agtagtaacg tcatcaccca acataattgg aatttcgttt acaccaatat
     3721 gtaagtaatc gtcgacagga tctaaataca gtggaacatc acctgcttcc atacgtgcag
     3781 gattgagtgg
//
LOCUS       DAOQHY010000254         3783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_14969_length_3783_cov_3.795333, whole genome shotgun sequence.
ACCESSION   DAOQHY010000254 DAOQHY010000000
VERSION     DAOQHY010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14969_length_3783_cov_3.795333"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="PLL28_14085"
     CDS             complement(<1..1050)
                     /locus_tag="PLL28_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70507.1"
                     /translation="MEKITKLVHSLSNREVKLFYKYNALSGKKTNNLKIRLFEIAKQN
                     PEISDIEAAKILGKKVDAAFSMLKHRLQEDIYRILVFSVDESKFYSKTFKARYEVFKL
                     LAQSTLLDSRNLKQLATEAIQKADRIAQEYELLSSRVIINEILINSHQAAQGFRTYNK
                     FKETVFSDISKQQALATGQDLFKQLIVPNMFAANRKKSQAEIAKNSAEQLEQLNKIHP
                     SVRLNFLLLRSQMEYAEITQDFQTYYNTASEFLKLVTNNPPVSTSDNIGGANMLLAES
                     SLRLHNWDNSKIFINESEKYFFKNSNNYITLMSIKYSMLVQTKSYEDAYQLVQEIRQL
                     KSIKRGSFQYSKWIYY"
     gene            1240..1392
                     /locus_tag="PLL28_14090"
     CDS             1240..1392
                     /locus_tag="PLL28_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70508.1"
                     /translation="MSIIFAILMSLGIISNPVNSDVKHDGINQQKFDYTYSTNAKGGL
                     DWEDTH"
     gene            1624..2022
                     /locus_tag="PLL28_14095"
     CDS             1624..2022
                     /locus_tag="PLL28_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70509.1"
                     /translation="MVHTLQRHDSLEAALLAPIRDYLAGIYCPADGWKLFNRFQWRTK
                     VPDMIIQKQGKTGFERVIVSVHLENGASRQHFSEMEKLSSRLQKDTVGRMKKILVVDN
                     AAPLDLPADVEVVNLRDLLKIRATEPKLVA"
     gene            complement(2033..3247)
                     /locus_tag="PLL28_14100"
     CDS             complement(2033..3247)
                     /locus_tag="PLL28_14100"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNF70510.1"
                     /translation="MEEIKLNTIPEAIEDIRQGKLIIVVDDENRENEGDFITAARNVT
                     PEIINFMATHGRGLICAPLIEDRCDELELNLMVDDNTALHSTQFTVSVDLIGHGCTTG
                     ISTADRALTIKALINPEMGAKDLARPGHIFPLRAKKGGVLRRAGHTEATIDLARMAGF
                     EPAGVLVEIMNEDGTMARLTDLVKVAEKFDLKIISIKDLIEYRLQTETIIHKEVEVQL
                     PTKYGHFTLHAYTQTTTGEVHMALVKGKWQNEEAVLVRVHSSCATGDILGSLRCDCGD
                     QLHAAMHMIDKNGSGVLLYMQQEGRGIGLLNKLKAYKLQEEGRDTVEANLELGFPMDQ
                     RDYGVGAQILRDLGIRKINLITNNPKKRVALKGYGLEIMGNTPIEILPNAHNENYLLT
                     KRDKMGHEILKD"
     gene            complement(3261..>3783)
                     /locus_tag="PLL28_14105"
     CDS             complement(3261..>3783)
                     /locus_tag="PLL28_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70511.1"
                     /translation="NIPTNFLMEDFYITMHVIRSGSQAICDLDAKAYEDVSNLVEEEY
                     KRKIRISAGNFQNLGVYKGMLLKPLSGAGFSFLSHKVLRWIGPFLLIALLLSSCFLCG
                     KNLFYTLAFCVQILGLISPFIDKVLKRMNIHTFALRLVAYFYQMNMALLQGWWKYMTG
                     IRSATWNPTQRNV"
BASE COUNT          998 a          834 c          767 g         1184 t
ORIGIN      
        1 ataataaatc catttggaat attggaagct tcctcgtttg atagatttta attgacggat
       61 ttcttgtact aattgatatg catcttcata tgactttgtc tgtacaagca tcgagtattt
      121 aattgacatt aatgtaatgt agttgttgct gttcttgaag aaatattttt cagattcatt
      181 aataaatatt tttgaattgt cccagttgtg taggcgcagt gaagattccg caagcagcat
      241 attggcacct ccaatgttat cgcttgtaga cactggtgga ttgtttgtta caagctttaa
      301 aaattcactt gctgtattat aataagtttg aaagtcttgt gttatttcag catattccat
      361 ttgacttcgc agcaacaaaa aatttaacct tactgaggga tgaattttat tcaattgttc
      421 gagttgctct gctgaatttt tggcgatttc agcttggctt tttttcctat tcgccgcaaa
      481 catgttgggg actatcaact gcttgaaaag atcctgtccg gttgcaagtg cttgttgttt
      541 gcttatatca ctaaatacag tttctttaaa tttgttataa gttctgaaac cttgcgctgc
      601 ttgatgagag ttaataagaa tttcatttat tattaccctt gaactgagta attcatactc
      661 ctgtgcaatt cggtcagctt tttgaatggc ttcagtcgcg agttgtttca gattcctgct
      721 gtccaaaaga gtactttgtg caagcagttt aaatacctcg taccttgcct tgaatgtttt
      781 ggagtaaaat tttgattcat ctacagaaaa cactagtatt ctataaatat cctcctgcaa
      841 acggtgtttt aacatgctaa aagccgcatc aaccttcttt cccaaaattt tcgctgcttc
      901 tatgtcacta atttcaggat tttgcttcgc aatttcgaat agacgaattt ttaaattatt
      961 cgttttctta ccgcttaagg cgttgtattt gtagaaaagt ttgacttccc ggttggaaag
     1021 actatgcact agttttgtta ttttttccat acttttgaaa cgaattgttt agtcacaatt
     1081 tctacttttg ggttcgttaa ttacacacgc acaccccaac cacacacacg agtatttgtt
     1141 taattgtcaa cagcatattc tgttgaaggg tctgtaattg ctatttatat taaaaataaa
     1201 catattcatt ttcactcaac acttctaaac cgcaacacta tgtcaatcat cttcgctatc
     1261 ctgatgtcac ttggaatcat ttccaaccct gtaaactctg acgttaaaca cgatggtatc
     1321 aaccagcaaa aatttgatta tacctattct acaaatgcca aaggcggtct tgactgggaa
     1381 gatacccact aatcaaaacc tccactatgc ttagttgatg catcatttca tgcatcaaat
     1441 atactaaact gttaattatt taacggtaaa aaatacctat tgtcctgtaa atcaggatga
     1501 tacaatcctg cattgttcaa aaaacgatgt tagggaagct attatttttc cttgtggcgg
     1561 ccaccgtatc gcatgatctt agcatcagca attgcaatta agcattgcct aaacgaaacg
     1621 ggtatggttc acacactaca acgacacgat tccctggaag ctgccttgct cgccccgata
     1681 agggattatt tagcaggtat ctattgtccg gcagatggct ggaaattatt caaccgcttt
     1741 caatggcgca cgaaggtgcc cgacatgatc atccagaaac agggtaagac cggtttcgag
     1801 cgagtgattg tgagtgttca tcttgaaaat ggtgcttcac gacagcactt cagtgagatg
     1861 gaaaagcttt cttcccgcct gcagaaggat actgtaggtc gcatgaaaaa aatattggtg
     1921 gtagacaatg ccgcaccgct tgatctgccg gcagatgtag aggtggtaaa cctccgcgat
     1981 ctgttaaaga tacgggccac ggaacccaag cttgttgctt gatttaaccc ccttaatcct
     2041 ttaggatttc gtggcccatc ttatctctct tagtcaggag atagttttca ttgtgtgcat
     2101 tgggcaatat ctctatcggg gtattgccca ttatttctaa accatatcct tttagcgcca
     2161 cacgcttttt aggattgttg gtgatgagat taatcttgcg tatgcccagg tcgcgaagta
     2221 tctgagcacc cacgccgtaa tcgcgctgat ccatcgggaa gccgagctcc aaattggctt
     2281 ccacggtgtc gcgcccttcc tcttgcagtt tgtaagcctt aagcttattc aatagcccaa
     2341 tgccgcggcc ttcctgctgc atatataata atacaccgga gccgtttttg tctatcatgt
     2401 gcatggctgc atgtagctga tcgccgcaat cgcaacgcaa agaaccgagg atatcgcccg
     2461 tggcgcaact gctgtgtacg cgcaccaata cagcttcttc gttttgccat tttcctttca
     2521 caagcgccat gtgcacctcg ccggtagtgg tctgggtgta ggcatgtaag gtgaaatgac
     2581 catattttgt gggcagttgt acctccactt ctttatggat gatggtttct gtttgcaggc
     2641 gatattcaat aaggtctttg atgctgatga tcttcaggtc gaatttctct gccaccttca
     2701 ccaggtcggt aagacgtgcc atggtgccgt cttcattcat gatttccacc aacacccctg
     2761 caggctcaaa tcccgccata cgagcaaggt ctatggttgc ctcggtatgt cctgcacggc
     2821 gcaatacgcc gccctttttt gcacgcagcg gaaaaatatg tcccggacgg gcaaggtctt
     2881 tggcacccat ttccgggttt atcaatgctt tgatggtcag ggctctgtcg gcggtggaga
     2941 tacccgtggt gcatccatgc ccgatgagat ctactgatac ggtgaattgc gtgctatgta
     3001 aagcggtatt gtcatccacc atcagattta attccagttc atcacatctg tcttcaatca
     3061 gcggagcgca gataaggcct ctgccgtggg tagccataaa attgatgatt tcgggggtaa
     3121 cgttgcgcgc ggcggtaatg aaatcgcctt cattttcacg attttcgtca tccaccacaa
     3181 taatcagttt accttgacgt atgtcttcaa tagcttcggg aatagtattc agcttaatct
     3241 cttccattgt ttcattctgt ttaaacgttg cgttgcgtcg gattccaggt ggctgagcgt
     3301 atgccggtca tatatttcca ccatccttgc aacaaggcca tattcatttg atagaaatag
     3361 gccaccaatc ggagtgcaaa ggtatggata ttcatccgct taagcacctt gtcgataaaa
     3421 ggtgaaatta aaccaagtat ctgcacacaa aacgccagcg tataaaataa gttcttaccg
     3481 cataaaaagc atgatgacag caataatgca atcaacaaaa acggccctat ccagcgcaat
     3541 accttatgcg acaaaaagct gaatcccgca ccactcaaag gtttcaggag catgccttta
     3601 tacacgccaa ggttttgaaa attgcctgcc gaaatgcgaa tcttgcgctt gtattcctct
     3661 tccaccagat tgctcacatc ttcgtatgct ttggcatcca gatcgcaaat ggcctgactg
     3721 ccggagcgaa tgacgtgcat ggtgatataa aaatcttcca tcagaaaatt ggtaggaata
     3781 ttg
//
LOCUS       DAOQHY010000255         3746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_15218_length_3746_cov_6.475210, whole genome shotgun sequence.
ACCESSION   DAOQHY010000255 DAOQHY010000000
VERSION     DAOQHY010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15218_length_3746_cov_6.475210"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PLL28_14110"
     CDS             <1..614
                     /locus_tag="PLL28_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HNF70512.1"
                     /translation="MAMQSVGKAAMSMIQEVRRQFNEIPALKAALDAMKRNGDKEVHE
                     WSKADQEIFEAANGKAEYAKCVEISTKAAVKEMVVPGLMAVIVPVIVGFAPKVLGLNT
                     EGHELMGPEMLGGLLAGVTVSGVLMAIFQANAGGAWDNAKKMFEEGVDINGKTYYKGS
                     DPHKAAVVGDTVGDPFKDTSGPSINILIKLMSVVSLVLASLMV"
     gene            694..2109
                     /locus_tag="PLL28_14115"
     CDS             694..2109
                     /locus_tag="PLL28_14115"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70513.1"
                     /translation="MRRIFPWVLVCLYPFAMHAQIDTVTLTEDGHFYFENFLPYSAYS
                     SLMNRNGEPYLYAACRELGVVTFDISDPDFPVPVDTIPTTALNNLIPNNISQTGDYLY
                     VATGGFNLIPQKAGLAIMNLADAAHPVITDKWDSVAYNQGAAIAISDGNYAYLGGMDD
                     GLIILDVSNKSNITFKSSILPDPNYPEVPDLFSVPNVRGLALYGTDTLMVAYDAGGLR
                     MLDVSNKSAPIEIGKYADPDIENIAQSAYNNIAIKQHYAYIPVDMCGLLVVDIADSDM
                     STVAWFNPWDCDSTNWNGRPGHTNEVRINEDEDLLFVSGGDSELLIFDISNPATPTLK
                     ATYGVPFDSIVAWSLDVFGNQISLALVDNSFVGFPYYSDVGGIQLLQYEAIHTIDIQS
                     LYKEFKVYPNPSTGVIVIDDIPSGSAMRIMNLQGVICEPEINGNVIDCSSLASGIYIL
                     QLHDIQGRLIGSSKIVLTGVN"
     gene            complement(2407..>3746)
                     /locus_tag="PLL28_14120"
     CDS             complement(2407..>3746)
                     /locus_tag="PLL28_14120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNF70514.1"
                     /translation="QIAVDGSNVYILYGTSWTDLVLLTSTSNGDPGSWTITTIINTGF
                     CNYQGDLDQTSDVTGDGIADTVETTDGFHEMVLDDAGVVHVWSGYYWLLDDDPATEGW
                     SYFPSLYGLWYWNSTMAAESPMWIDLLVDWDDSGDAFDGIGADLGMYDGVTFTSMPTA
                     AIDEDAGRIYLAYTMPIEYTDYFDDPTVAEAQSFRDLFGVYSDDMGLSWSAPVNMTYT
                     AHDNQEVSFPYAFDRFLNGCMYTIWHQDDEPGTALDVGQVAADVNSIANMQFRCFDEA
                     RFNPYPPTAEYDYTPDGTTGLVNFTNLSVDADTYSWDFGDGGSSTAKNPAHVYATSGV
                     FNVCLTAMNKYDDDNACKVIDITVGVVDYALGQALSVYPTPASTNVTVEVNGNFGTLY
                     AEVYNALGERVINTTAFNGSVNFDVTALSAGNYIVKVATVDGKYTSRQISVSK"
BASE COUNT         1140 a          722 c          822 g         1062 t
ORIGIN      
        1 caatggccat gcaaagtgta ggtaaagctg ccatgagcat gattcaggag gtgcgccgac
       61 aattcaacga aattccggct ttaaaggctg ctcttgatgc catgaaacgc aatggcgata
      121 aagaagtaca tgagtggtct aaagcagatc aggaaatatt cgaagcggca aacggaaaag
      181 cagaatatgc aaaatgtgtg gagatttcca ctaaagctgc cgtaaaagaa atggtggttc
      241 ccggattaat ggccgttatt gttcccgtaa tcgtaggctt tgctccgaaa gtacttggac
      301 tcaataccga aggtcacgaa ctaatgggtc ctgaaatgtt gggtggcttg cttgcaggag
      361 taactgtaag cggtgtgctg atggcaattt ttcaagccaa cgcaggtgga gcctgggata
      421 atgctaaaaa gatgtttgaa gaaggggttg atattaacgg aaaaacctac tacaaaggca
      481 gtgatccaca taaggctgcc gttgttggcg ataccgttgg tgatccattt aaagatactt
      541 ccgggccatc tatcaatatc ctcattaaat taatgagcgt tgtgagcttg gtgcttgcgt
      601 ctttgatggt gtaagctaat tttatcaata cttgaatagc cctgattcca cgagtcaggg
      661 cttttttttg cacggttttt gtaaattaga ggtatgagaa ggattttccc atgggtattg
      721 gtatgtctgt acccttttgc aatgcatgcc caaattgata ccgtcacact cacggaagat
      781 ggtcattttt atttcgaaaa ttttcttccc tattcggcat attcgtcact gatgaataga
      841 aatggtgaac catatttata tgcagcttgt cgcgaacttg gtgtggtgac cttcgatatt
      901 tcggatcctg attttccggt gcctgttgat accattccca ctaccgcatt aaataatctc
      961 attcctaata atatttcgca aaccggcgat tatttatacg ttgctacagg tggctttaat
     1021 ctcatccctc aaaaggcagg actagccatt atgaatcttg ctgatgcagc acatcctgtt
     1081 attaccgaca aatgggattc agtagcttat aatcagggcg ctgccattgc cattagcgat
     1141 ggtaattacg cataccttgg aggtatggac gatggattga taattctgga tgtgagcaat
     1201 aaatcaaata tcacattcaa atcatccatt ctccctgatc caaattatcc tgaagtgccg
     1261 gatttgtttt ctgttccgaa tgttcgtgga ttagcacttt atggcactga tacactcatg
     1321 gttgcttatg atgcgggtgg attgagaatg ttagacgtga gcaataaatc agcaccaatt
     1381 gaaatcggaa aatatgccga tccggatata gaaaatattg cacagtcggc gtataacaat
     1441 attgccatca aacaacatta tgcctacata ccggttgaca tgtgtggttt acttgtagta
     1501 gatatagcag atagtgatat gagtacagtg gcctggttta atccctggga ttgcgattct
     1561 acaaactgga atggcaggcc gggacataca aatgaagtgc ggataaatga ggatgaagac
     1621 ttattatttg taagtggtgg cgacagtgaa ttattgattt ttgatatctc aaatccggca
     1681 acgcctacac taaaagccac ttatggcgtg ccctttgata gtattgttgc ctggagtctg
     1741 gatgtatttg ggaatcaaat ttcacttgca ttagtcgata attcgtttgt aggctttcct
     1801 tattattctg atgtaggtgg cattcaatta ttgcaatatg aagcaattca tactattgat
     1861 attcaatcgt tgtataaaga atttaaggtg tacccaaatc cttctacggg agtcattgtt
     1921 atcgatgata ttccatcagg cagcgcaatg agaataatga atctgcaggg tgtaatatgc
     1981 gagcctgaaa taaatggaaa tgtaattgat tgcagcagtt tagcttcagg aatatatatt
     2041 ttgcagttgc atgatattca aggtcgactt attggtagca gtaagattgt attgacggga
     2101 gtaaattaaa cgatggaatt cgtacatcgt ttatcatgat tttctttatc caataaaaaa
     2161 ccccggcatt gctgtcgggg cctccacatc aaaaaaacat aaagcacttg ttgttggctt
     2221 gttcatgctt tatgtaaaat gtcatctaac ctaactggtc tgttgtggtt gtgcagacta
     2281 tgctaaacta catcgtaata aatgcctaag gaaaagtggc aacctagttt aagaaacctt
     2341 aaatgttaat taacaaaaaa gccccgacat tgctgtcgag gcttttatgc tattttgaag
     2401 caaatattat ttgcttacag agatttgacg gctagtgtat ttaccatcaa cagttgcaac
     2461 ttttacgata tagttacctg cagaaagagc agttacatcg aagttaacag aaccgttgaa
     2521 agcagttgta ttgattacgc gttcgccaag tgcattatat acttcagcat ataatgttcc
     2581 gaagttacca ttaacttcta cagttacatt agtagatgca ggagttgggt aaacagaaag
     2641 agcttgacca agagcatagt caacaacacc aacagtgatg tcgattactt tacatgcatt
     2701 gtcgtcatcg tatttgttca tagcagtaag acatacgttg aacacacctg aagttgcata
     2761 aacgtgagca gggtttttag cagttgaaga accgccatca ccgaaatccc aagaatatgt
     2821 atcagcatct acagataagt ttgtgaagtt cacaagacct gtagttccgt caggagtata
     2881 atcgtattct gcagttggtg gataagggtt aaaacgagcc tcatcgaagc aacggaattg
     2941 catattagca attgagttta catcggcagc aacttgccca acgtcaagag cagttcccgg
     3001 ctcatcatcc tgatgccaaa tggtatacat acaaccattt aggaaacgat cgaaagcgta
     3061 agggaaagaa acttcctgat tatcatgagc ggtataagtc atgttaacag gagcgctcca
     3121 agaaagaccc atatcatcag aataaacacc gaaaagatcg cggaaagact gagcttcagc
     3181 tacagttgga tcatcgaaat agtcggtgta ttcaattggc atagtgtaag cgaggtagat
     3241 acgacctgca tcttcatcga ttgcagcagt aggcattgag gtgaaagtaa caccatcata
     3301 cataccaagg tcagcgccga taccatcaaa agcatcaccg ctatcatccc agtcaaccaa
     3361 aagatcgatc cacattggtg attcagcagc catagttgag ttccagtacc acaaaccgta
     3421 gaggcttggg aagtagctcc aaccttcagt agccggatcg tcgtcgagca accagtagta
     3481 accagaccaa acgtgaacaa caccggcatc atcaagaacc atttcatgga aaccatcagt
     3541 agtttcaact gtatcagcaa taccatcgcc ggttacatct gaagtttgat cgaggtcacc
     3601 ttgatagtta cagaaacctg tattgatgat agtagtgatt gtccatgaac caggatcacc
     3661 gttagaagtt gaagtcaaca acacgagatc agtccatgaa gtaccgtaaa ggatgtaaac
     3721 attgctacca tcaacagcga tttgat
//
LOCUS       DAOQHY010000256         3726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_15362_length_3726_cov_5.592209, whole genome shotgun sequence.
ACCESSION   DAOQHY010000256 DAOQHY010000000
VERSION     DAOQHY010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15362_length_3726_cov_5.592209"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="PLL28_14125"
     CDS             complement(<1..904)
                     /locus_tag="PLL28_14125"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HNF70515.1"
                     /translation="MRKIYLLLTMALVCVFSVSKAQVYAYLDDPTGTYAGVATNASGT
                     NLSRYNGLESDPACGVGFSSIKHTTSGYTNNRPGVQFTVTADPGFQLNVTSISVDVRR
                     NPKGPQFWRIAYSTDGGATWTNSGIDEAVASSGCFTSTNVVFDVADFSTTSTLTVRII
                     GHTAYSALNGISTLKNINVAGTVSFADADGDGYTSDVDCNDADAAINPGATEICNGID
                     DNCNGDIDEGAGATWYADADGDTYGDAASTTVACDMPEGYVADATDCNDADAAVNPGA
                     TEVCNGVDDNCNGDIDEGAGATWYA"
     gene            1310..1383
                     /locus_tag="PLL28_14130"
     tRNA            1310..1383
                     /locus_tag="PLL28_14130"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Ile,seq:cat)
     gene            1462..2700
                     /locus_tag="PLL28_14135"
     CDS             1462..2700
                     /locus_tag="PLL28_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNF70516.1"
                     /translation="MIHFQKKQLSNGLTVIVNEDHSTQLCAVNVLYKVGARDEVASMT
                     GFAHLFEHLMFGGSVNAPDFDTELQLAGGTNNAYTSNDLTNYYDVLPVSNIETALWLE
                     SDRMLQLDFSERSLEVQRNVVCEEFKEHYINRPYGDVWHILRELVYEKHPYRWPTIGL
                     ELQHVRDARLSDVEAFFYKYYRPNNAILTISGGITTEKAFSLAEKWFGDIPAGEDFIR
                     TYPTEPVQQQAKFLAVQKDVPVNVIYKAWKMPGRIEQGYYATNMLSDVLGTGEGSRLQ
                     HALKKEKKLFTDIAAFVTGSIDTGMFIISGKLAEGVSWDAAEEGIRIELEKIRSAHIT
                     EQELQRIKNAVEMDITGNNTGVLNKAESLAAAEMLEGADLVNTEMERFLRINTNDMLQ
                     AAQTILREEACSTLHYGNVN"
     gene            2709..>3726
                     /locus_tag="PLL28_14140"
     CDS             2709..>3726
                     /locus_tag="PLL28_14140"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNF70517.1"
                     /translation="MQIDRNKAPELQSIDRLDIRNYKSTALSNGTSVTYVGGVEQDLI
                     RVELIFHGGRWYEPSQGVSRATCNLMKKGTSTKSAKEIADSIDFYGAQLEVNAGQDFT
                     SVVIYCLNKHLASILPIAEEIMIDAAFRDEELELFVAKQKQKLRINAQNTDFHANRKF
                     NAVLFGNQHPYGYAMTAETLDALQSDVLRNYHRKHYNPGDARIFLSGNVTDDVLHLVE
                     KHFGHYTATGKIISATDKSIHTASEHKYFIDKDDSVQSSVRIGTLAISKTHPDFPEFN
                     VLNTLFGGYFGSRLMTNIREDKGYTYGIYSSISHYRHASYFSIETEVGNEVCASALEE
                     IYKEM"
BASE COUNT         1104 a          760 c          818 g         1044 t
ORIGIN      
        1 cggcatacca ggttgcgccg gctccttcgt cgatatcgcc gttgcaatta tcatcaacac
       61 cgttacatac ttctgttgct cccggattta cggcagcatc agcatcattg caatcggtag
      121 catcggcaac ataaccttca ggcatatcgc aagcaaccgt tgttgaagcc gcatcgccat
      181 aagtatcgcc atctgcatcg gcataccagg ttgcgccggc tccttcgtcg atatcgccgt
      241 tgcaattatc atcgattcca ttacagattt ctgtagctcc cggattgata gctgcatccg
      301 catcattaca atctacatca gacgtatacc catcgccatc agcatccgcg aaacttactg
      361 ttccggcaac gttgatattt ttgagggtag agataccatt cagagcgctg taagctgtgt
      421 gaccgatgat gcgaacagtc agcgtagaag tagtagaaaa atctgcaaca tcaaacacta
      481 cgtttgtaga tgtaaaacaa ccgctgctgg caactgcctc atcaattccg ctgttggtcc
      541 atgtagcacc gccatcagtt gaataggcaa tacgccagaa ctgtggtcct tttggattgc
      601 ggcgcacatc gacagaaata cttgtaacat tcagttgaaa accgggatct gcagtaacgg
      661 taaattgcac accgggacgg ttgttcgtat aaccggaagt ggtatgtttg atagagctga
      721 atcccacccc acaagcaggg tctgattcca gtccgttgta acgactaagg ttagtgccgg
      781 aggcattggt agcgacacct gcataggttc cggtcgggtc atctaaatac gcatacactt
      841 gtgctttcga gacagaaaac acgcacacta acgccatggt tagcaacagg taaatttttc
      901 tcatacagtt taaatttttg gtgaaagtgc gtgggaattc actgtggatg gctgcaagat
      961 attactgctt tggatagcaa gaaaggactt aacgattagt gaatgtcaca taatttgctg
     1021 attcataata tgtaattaaa tgattttcaa ctatttacat attagattgt atctcatgca
     1081 ttaattttgt gcagtcacaa agcttgagta ttttgaaata aattgcataa gaaatgtcac
     1141 aaaactcttc aactgagttg cgatgcattg aaagtctatt tgcctaaaat gggtattggc
     1201 gcaggaaaag agtgatgtcg tgagtaagaa gattagccat acgtttaaaa aacataggcg
     1261 aatgcctata tttgcactcc caaattattg taataacgaa caaattttcg ggcctatagc
     1321 tcattcggtt agagcaactg actcataatc agtaggtgcc tggttcgatt ccaggtgggc
     1381 ccactactcg aaaaggcgct tttggcgcct ttttcattgg aactacatga actttatctt
     1441 ccatttccca attttgcatc catgattcat tttcaaaaga aacaactgtc gaatgggctc
     1501 acggtgatcg tgaatgaaga tcatagcacc cagctctgtg cagtgaacgt attatataaa
     1561 gtaggtgccc gcgatgaggt ggcatcaatg accggattcg ctcacctgtt cgaacacctg
     1621 atgtttggcg gttcggtgaa tgcgcctgat ttcgatactg aattgcaatt ggcaggcgga
     1681 acaaataatg cctataccag caacgatctc acgaattatt atgatgtgct tccggtgagc
     1741 aatattgaaa ctgcactctg gctggaaagc gatagaatgt tgcaactcga tttttctgaa
     1801 cgttcattgg aagtgcaacg caatgttgtt tgtgaagagt ttaaagagca ttatatcaac
     1861 cgaccttatg gcgatgtatg gcatatctta cgtgaattag tttatgaaaa acatccttat
     1921 cgctggccaa caatagggtt agaattgcaa catgtgcgcg atgctaggtt gagtgatgtg
     1981 gaagcattct tttataaata ttatcgccct aacaatgcca ttctcaccat tagcggtggc
     2041 atcacaactg aaaaagcatt ttcgttggca gaaaaatggt tcggtgatat tcctgcaggt
     2101 gaagatttta tcagaacata tccgacagaa cctgttcaac aacaagctaa atttcttgcc
     2161 gtgcaaaaag atgttcctgt taatgtcatt tacaaagcat ggaaaatgcc gggacgcatt
     2221 gaacaaggat attatgctac taatatgctg agcgatgtat tgggaacagg cgaaggctcg
     2281 cgcttgcaac atgccttgaa aaaagagaaa aaattattta ccgatatcgc tgcattcgtt
     2341 acggggtcta ttgatacggg catgtttatt atttccggca agcttgctga aggtgtttca
     2401 tgggatgctg ccgaagaagg tattcgcata gaattggaaa aaattcgcag tgcgcatatc
     2461 actgaacagg aattgcagcg cataaaaaat gctgttgaaa tggatatcac cggcaataat
     2521 acaggtgtat taaacaaagc ggaatcgctc gctgctgctg aaatgctgga gggtgccgat
     2581 ttagtgaata ctgaaatgga acgcttcctg cgaatcaaca ccaacgatat gttgcaagcc
     2641 gcacaaacta ttttgcgcga agaagcatgc agcactttac attatggaaa tgttaattaa
     2701 ggaatcagat gcagatagat agaaataaag ctcccgaatt acaatcgata gatcgtttag
     2761 atattcgcaa ttataaaagc actgcattaa gcaacggtac atctgttact tatgtaggtg
     2821 gtgttgaaca agaccttatc cgcgtagaac ttatttttca cggcggcagg tggtatgaac
     2881 cttcgcaagg tgtgagtcgc gctacgtgta atttgatgaa gaaaggcact tcaacaaaat
     2941 cggcaaaaga gattgccgat agtatcgatt tttatggtgc gcaactggaa gtgaatgccg
     3001 gtcaggattt cacttctgtt gtaatttact gtttaaacaa acacctcgcc agtatacttc
     3061 ctattgcaga agaaataatg atcgatgctg ccttcagaga cgaggaactt gaattatttg
     3121 tcgccaagca aaaacaaaaa ctccgcatca atgcgcagaa taccgatttc catgcaaaca
     3181 gaaaatttaa tgccgtacta tttggcaatc agcatccgta tggttatgcg atgactgccg
     3241 aaacacttga tgcattgcaa agtgatgtgt tgcgcaatta tcatcgcaag cattacaatc
     3301 cgggagatgc gcgtattttt ctttccggca atgttactga tgatgttttg catttagtgg
     3361 aaaaacattt tggtcactat actgctaccg gaaaaattat ttctgctacc gataaatcca
     3421 ttcacactgc atcagagcat aaatacttta ttgataaaga cgactccgtt caatcatctg
     3481 tacgcatagg cacacttgct atttctaaaa cgcatcccga ttttccggaa ttcaatgtat
     3541 tgaatacatt atttggcggt tatttcggtt cgcgactgat gacaaatatt cgtgaggata
     3601 agggatatac ctatggaata tattcttcca tttcccatta ccgccatgcg agttatttct
     3661 cgattgagac cgaagtaggt aatgaagtgt gcgcctcggc actggaagaa atctataaag
     3721 aaatgg
//
LOCUS       DAOQHY010000257         3701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_15541_length_3701_cov_5.217224, whole genome shotgun sequence.
ACCESSION   DAOQHY010000257 DAOQHY010000000
VERSION     DAOQHY010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15541_length_3701_cov_5.217224"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PLL28_14145"
     CDS             complement(<1..280)
                     /locus_tag="PLL28_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70518.1"
                     /translation="MKTNKRTSIKPIAIYILLLIFIGSNLFSAIGKNTESYRKNELTF
                     QKSGSTLIVFPDSVPVELDTSTAFEIGDTIDINLAGLDTLFILYEDTNS"
     gene            complement(571..831)
                     /locus_tag="PLL28_14150"
     CDS             complement(571..831)
                     /locus_tag="PLL28_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70519.1"
                     /translation="MRYKLTILNACSAAFMIGLIIYAMTNYSDLKNEDGFGMAAVYGL
                     AGLSLMGGMMDFLLQALVKHEGTMNILGSFVAITIGMVVALS"
     gene            complement(926..1576)
                     /locus_tag="PLL28_14155"
     CDS             complement(926..1576)
                     /locus_tag="PLL28_14155"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNF70520.1"
                     /translation="MIGIIRHNKEFYLLWAITMIIVGAILPFTEKSDFSLWLNFRHTQ
                     AADFFFRYATWLGDGFVIGVICLILFILKLRWGIIASVGSFFSALFINLIKKEYNEPR
                     PSVVLKNMDLHYVKGLDLYSNFSFPSGHTAAAFTLCVLLCYFAQDKRYTRLFFALAVI
                     VAISRVYLLQHFLVDVYFGALLGTIFGTILLWPFIRIPLFSRPWSEIGILSGNRKV"
     gene            complement(1573..3000)
                     /locus_tag="PLL28_14160"
     CDS             complement(1573..3000)
                     /locus_tag="PLL28_14160"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNF70521.1"
                     /translation="MTKIDKYIIKSFLGPFIISFIIALIIFLMIFLWKYIEDLVGKGL
                     EWYVIMKLLFYASANMVPLALPIAVLLACIMTYGNLGERLELAALKSAGLSMFTFMRS
                     TLLIGVFIAIGAYFFNNYVLPSAYLKFVVSLVDITKQKPALNIKPNEFYNEIDGYSIL
                     IGGKSDDNVTIHEVTIYEELDHSNDNVLIAESGKMQTTSDNNNLVFSLKNGKQYEQLA
                     PTGKGKRHVQHVRMHFAEWNKSLDLSGFQMTHTSEELYQNHFKMLNTHQLSHAIDSLK
                     TNNNNQAQALAPTLRVSYLQGRAPGVTFNDTAYAHQASSFIELIPLAQRLPVIKKSLQ
                     SVRTQTGYTNQVLMRMKNTRTKINSHLIELNRKYTLPLSCLLFVFIGAPLGAIIRKGG
                     FGLPVLLSIIIFIFYYVLNITGEKMAEQSALSPFVGMWLSSFILVPICVFLTIKAKND
                     APIVDGEWYYNQLVKLKGVFSKIKS"
     gene            3003..3590
                     /locus_tag="PLL28_14165"
     CDS             3003..3590
                     /locus_tag="PLL28_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70522.1"
                     /translation="MHIFDPAEAALFYNPDVFAVKRSATQKLYAAFEDLRLRLKDTGE
                     QLQFPFPNEVTSISAKISQGENYGGCPWVMLDYHRYFKERDMFAFRVMAWFGHYFSAH
                     LVLGGRFINVYNRNVKDNLEGSDVFFTLHDTPWEHAVGTPISLPLKGLTPAQISTQAD
                     EHQFVKYSICIPSADLSKVIDEVVAFYQSSFNTVT"
BASE COUNT         1137 a          687 c          760 g         1117 t
ORIGIN      
        1 tagaatttgt gtcttcataa agtatgaata atgtatctaa tcctgctaaa ttaatatcaa
       61 tagtatcgcc tatttcaaaa gcagtgctgg tatcgagttc gacaggaaca gaatccggaa
      121 atacaattaa tgtacttccc gatttttgaa aagttaattc atttttacgg taggattctg
      181 tgttcttgcc aatcgcacta aacaagtttg atccaataaa aatcaatagc aatatgtaaa
      241 ttgcaattgg ttttattgaa gttcgtttat ttgttttcat aaggtgtatt ttaataaaat
      301 tatgaataaa tttaataata aattaataaa tggtattttt tacctagcac tgataaatga
      361 ggtattgagc cgactcagga attacattat actattttct tattgttgag taattgctgt
      421 tacatattga aatggtattt ggaaaaaaag taattcagaa tacaatatta ttaaattact
      481 ggttttaaat taataacaag ctgtgcaact aaatatttta atacattcag tgtcgggtcg
      541 tttatgacaa aagtggggga atttgatctg tcaactcaat gccaccacca tcccgatagt
      601 aattgccaca aatgagccca ggatattcat ggtgccttca tgtttaacaa gagcctgtaa
      661 caggaagtcc atcatgcctc ccattaaact tagccctgcc agtccataaa ctgccgccat
      721 accaaagccg tcttcatttt ttaaatcgct gtaattagtc atggcgtaaa taatcaatcc
      781 gatcataaaa gctgcagagc aggcattgag aatggtgagc ttatagcgca tagcaataga
      841 ttgtttggat tagcacatag ctaatttaac atatttgcct aaacatagac acatagagta
      901 aggaattgat aattttcggc cgggatcaca ctttccggtt cccggatagg atgcctattt
      961 ctgaccatgg ccgagagaaa agcggaattc ttataaaagg ccagagcagg attgtgccga
     1021 agatggtgcc taacagtgcg ccgaaatata catcaacaag aaagtgctgc aacaagtata
     1081 ctctgcttat tgcaactatt acagccagcg caaaaaacaa gcgtgtgtag cgtttatcct
     1141 gcgcaaagta gcaaagtaaa acacataatg taaaggcggc ggcagtatgc cctgacggga
     1201 agctgaaatt gctgtaaaga tctagtcctt ttacataatg cagatccata tttttcagca
     1261 caacgctcgg ccttggttcg ttatattctt ttttgatgag gttgataaac aatgcgctga
     1321 aaaaactgcc aacggaagct attattcccc agcgcaattt taatatgaat agaattaaac
     1381 aaattacacc aattacgaaa ccatcaccaa gccatgtggc atatctgaaa aaaaagtcag
     1441 ctgcttgcgt gtgacgaaaa ttcagccata aagagaaatc ggatttttcg gtgaaaggta
     1501 gtatcgctcc cactatgatc atagtaattg cccatagcag gtaaaattct ttgttatggc
     1561 ggatgatacc tatcatgatt taatcttcga aaacactcct tttaatttaa ccaattgatt
     1621 gtaataccat tcaccatcaa caataggagc atcatttttg gctttgatgg tgaggaatac
     1681 acatatcggg actaaaataa atgaggataa ccacatgcca acgaatggcg ataaggcaga
     1741 ttgctcagcc attttttcgc cggtaatatt caggacgtaa taaaagataa atatgatgat
     1801 acttaataat accggcaatc caaatccgcc tttgcgtatg atggcaccta atggcgcacc
     1861 aatgaataca aacaataaac aggataatgg caaggtatat tttctattaa gttcgatgag
     1921 atgcgagtta atcttcgtgc gtgtgttttt catgcgcatt aaaacctgat tggtataacc
     1981 tgtttgtgta cggacacttt gcaaactttt tttaatgaca ggtaagcgtt gcgccaatgg
     2041 aataagttca ataaatgatg atgcctgatg agcgtaagca gtatcattga atgttacacc
     2101 aggtgctcgt ccctgtaaat acgaaacccg aagggtggga gcaagtgctt gcgcctgatt
     2161 attgttattg gttttcagtg agtcaatggc atgcgacaat tgatgcgtgt tcagcatctt
     2221 gaaatggttc tgatagagct cttcgctggt atgtgtcatc tgaaagccac tgagatcgag
     2281 cgatttgttc cactcagcaa aatgcatgcg cacgtgttga acatggcgtt taccttttcc
     2341 ggtaggcgct aattgttcgt attgcttgcc atttttcagt gagaaaacta aattgttatt
     2401 atcgctggtg gtttgcattt taccgctttc ggcaatgagc acattatcgt tggagtgatc
     2461 caattcttca taaatagtta cttcgtgaat agtaacatta tcatcacttt ttccgccgat
     2521 taaaatacta taaccatcaa tttcattata gaattcattt ggtttgatgt tgagtgccgg
     2581 tttttgtttg gtgatgtcga caagggagac aacgaatttc aggtaagcac tgggaagtac
     2641 atagttattg aaaaaataag cgccaatcgc aataaacaca cctatcagta atgtgctgcg
     2701 catgaaggtg aacatcgaca atcctgcact tttcaacgcg gcaagttcaa gtcgttctcc
     2761 aaggttgcca taagtcatga tacatgccag cagcacggca ataggcaatg caagcggcac
     2821 catattggcg gatgcataga acagcaattt catgattaca taccattcaa gccctttgcc
     2881 taccagatcc tcaatatatt tccaaaggaa tatcatgagg aatatgatca gtgcgatgat
     2941 aaaactgata atgaagggac ccaggaatga tttaatgata tatttgtcga tcttcgtcat
     3001 ctatgcacat ctttgatcct gccgaggctg cattgtttta caatcccgat gtttttgctg
     3061 ttaaacgcag cgcgacacag aaactgtatg cagcctttga agacttgcgc cttcgtctca
     3121 aagatacggg ggaacaatta caatttccgt tcccaaatga ggttacctcc atatctgcaa
     3181 agatctcaca gggggaaaat tatggtggtt gtccgtgggt gatgctggat taccaccgat
     3241 atttcaagga gagggacatg tttgccttca gggtaatggc gtggtttgga cattatttca
     3301 gtgcgcatct tgtgttggga gggaggttta tcaacgttta caacagaaat gttaaggaca
     3361 atttggaagg cagtgatgtt ttttttacgc tgcacgatac cccttgggaa catgctgtcg
     3421 ggacgccgat tagtttgcct ttgaaaggtt taacacctgc acaaatttca acgcaagcag
     3481 atgaacatca gtttgtaaaa tattcgattt gcattccatc agctgattta tccaaagtca
     3541 ttgatgaggt agttgccttt tatcagagca gttttaacac agttacataa tggtttttcg
     3601 ccgcaagcag cccttgactt ttggtgctac ctttttgtca agaaaaaggg tagcggagag
     3661 ttttttgcga agcgaagttt tcgtttcatc gaattaatac t
//
LOCUS       DAOQHY010000258         3640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_15983_length_3640_cov_4.946722, whole genome shotgun sequence.
ACCESSION   DAOQHY010000258 DAOQHY010000000
VERSION     DAOQHY010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15983_length_3640_cov_4.946722"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2541)
                     /locus_tag="PLL28_14170"
     CDS             complement(<1..2541)
                     /locus_tag="PLL28_14170"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HNF70523.1"
                     /translation="MYRNLRKLALVIALVPCMCMLQAQNSSLMWEITGNGLKEPSYIY
                     GTMHVSDKIAFHLTDSFFIAVKNADVIALESNPEHWIENDFGNPDMSSNYEFDESVGY
                     SGYDGSNMYFGGSDFYTDATEVFIPDQRTYYSAFADNSWLINGYLYRYADQRGDYEEE
                     TYLDLFIFQCGKKLSKQIAPLEREQEVLRLLEKANAEPDKDQKEAENAEFLEQERMRA
                     KLEEDGKDIYELIDEAYRAGNLDKLDSLERVVSPSRHYHYYFIEERNRNMMRRMDSIL
                     QTGKSMFTGIGAAHLPGQNGAIEILRSMGYTVRPVMGKVNNKSIKEKEKIDGIYFAQP
                     TKFQYAYDSLFTIESPGKLYEIGGYQTNSFFMYPDMANSAYYAILRVAHHAYLRNKTP
                     QDVYNEIDEMLYENIPGDIQSKTKITTNNGWPGFDIISKTKRGNYLRFKIVVSPVETI
                     IFKASGIGDFMIKAKEPETFFSSIKFRNTNSNAWSNYTPEWGGLSVSLPDIRVTDDVP
                     DGASVSNAEIVSIGEGVAGENKYWMSTYYLNDIKYIEEDSFELVRIADVFAKQFKKQD
                     YKNQGYKFSTYQGKPACTGVWKSAQGTYLHTQSVINGPYYIFMTAFTNNNTVPSDYFN
                     SLKLIDLTYNQEFTTYTDTSLYFTVKTIFPEPDTLLTLEEVDMGGYNDYGYNDDDDED
                     EIDEDYLELTRKTYFSSETTPENIYVVYHKYNDYTYFKNKKAFWNSFDTVYSSSFNLT
                     RRKFSEKDDIQSLEFLLTDTNSTRGIYKKFILKDNAIFQLSTCIDTTKAPSLFVSNFY
                     NTFTPSDSVDGRSVFASPADNFFAALDGTDTMKRKQAIAAVDIVNLDNS"
     gene            2678..>3640
                     /locus_tag="PLL28_14175"
     CDS             2678..>3640
                     /locus_tag="PLL28_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNF70524.1"
                     /translation="METTIRYDFSLLTEYDIHLFKQGRHFRLFEKLGAHPMSGEGQEG
                     VYFAVWAPNAKGVCVAGTFNHWNTTALPLQCRTDGSGIWEGVVSNVMGGDLYKFIING
                     ANGDVFEKADPFARYAEPAPGTASIVWDSTYTWEDALWLTKRKPFTFDKPMSMYEVHL
                     GSWGRQPDDANAFLTYRQLAETLVPYVKEMGFTHVELLPVTEHPFYGSWGYQTLSFFA
                     PTSRYGTPQDLMYLIDSFHKAHIGVILDWVPSHFPQDGHGLAFFDGTHLFEHEDRRKG
                     FHPDWNSYIFNYGRFEVKSFLISSALFWVDQFHFDGIRVDAVASM"
BASE COUNT          997 a          791 c          734 g         1118 t
ORIGIN      
        1 gctattgtcg agattcacaa tatcaacagc agctattgcc tgcttgcgtt tcatcgtatc
       61 agtgccatcc agtgccgcaa agaaattatc agccggactg gcaaacacac tgcgaccatc
      121 aacgctgtca gaaggggtga aggtgttata gaaattgctg acaaataaag aaggcgcctt
      181 tgtagtatca atgcaagtgc tcaattggaa gatggcattg tctttaagaa tgaatttctt
      241 ataaatacct cttgtgctgt tcgtgtcggt aagtaaaaat tcaagtgatt ggatatcgtc
      301 tttctctgaa aattttcgtc tggttaaatt gaatgatgac gagtaaaccg tatcgaatga
      361 attccaaaac gcttttttat ttttgaaata cgtgtaatca ttatatttat gataaacaac
      421 atagatgttt tcaggtgttg tttctgatga gaaataggtt tttcttgtta attcaagata
      481 atcttcatca atttcatcct catcgtcatc atcattatag ccgtagtcat tatagcctcc
      541 catatcaact tcctccagtg taagcaaagt gtcgggctca gggaaaattg ttttcacagt
      601 gaaatacaaa ctagtatctg tataagttgt aaattcctga ttataggtga ggtcaatgag
      661 ttttaatgaa ttaaaataat cacttggcac agtattgtta ttggtgaatg ccgtcatgaa
      721 aatatagtag gggccattta ttaccgactg cgtgtgtaaa taggtgcctt gtgcgctttt
      781 ccatacgccg gtacaagccg gcttaccttg ataagtgctg aatttatacc cctgattttt
      841 gtaatcctgc tttttgaatt gtttcgcgaa aacatctgca atacgcacaa gctcaaagct
      901 gtcttcttct atatatttga tgtcgttgag atagtaagtc gacatccagt atttattttc
      961 accggccacg ccttcaccta tggaaacaat ttctgcattg ctgacgcttg cgccatcagg
     1021 aacatcgtct gtaacacgta tatccggcag cgacacactt aatcctcccc attcgggtgt
     1081 gtaatttgac caggcgttgc tgtttgtatt tcgaaatttt atagacgaga aaaatgtttc
     1141 cggttcttta gccttgatca tgaaatctcc aataccactc gctttgaaaa tgatggtttc
     1201 aacagggctt accactattt taaaccgcag ataatttccg cgcttggttt tactaattat
     1261 atcaaatccg ggccaaccat tattggtagt gatttttgtt ttactttgaa tatcacccgg
     1321 aatattttca tataacatct catcaatttc attatataca tcctgaggtg ttttattgcg
     1381 aagataggcg tgatgggcaa cgcgcaaaat ggcgtagtat gcgctgttcg ccatatccgg
     1441 atacatgaaa aatgaattgg tctgatagcc ccctatttca tacaattttc ccggagattc
     1501 aatggtgaat agtgagtcat aggcatattg aaattttgta ggttgggcaa aatatatgcc
     1561 atcaattttc tctttttcct taatgctttt attgttcact ttacccatta ccggacgaac
     1621 agtatatccc atgctgcgca aaatttcaat cgcaccattc tgaccgggca ggtgagccgc
     1681 accaatgcca gtgaacatcg atttgcctgt ctgcaaaatg gaatccattc tgcgcatcat
     1741 atttctgttg cgttcctcaa taaaatagta atgataatgg cgcgaagggg atacaacgcg
     1801 ttccagtgaa tccaatttat cgagatttcc tgcgcgatac gcctcatcaa tcaactcata
     1861 aatgtctttt ccatcttcct ccaacttagc acgcatgcgt tcctgctcca ggaattcagc
     1921 attttctgcc tccttctgat ctttgtcagg ttctgcattt gctttttcta ataatcgtaa
     1981 tacttcctgc tcgcgctcca atggtgcaat ttgttttgat aactttttac cgcactgaaa
     2041 aatgaacagg tccagatagg tttcctcctc ataatcacca cgctgatcgg catagcgata
     2101 gagataacca tttatcagcc agctgttatc ggcaaaggca gaataatatg tgcgctgatc
     2161 aggaataaat acttcagttg catcggtata aaaatcactt cccccgaaat acatattact
     2221 cccatcgtat ccgctatatc ctactgattc gtcaaattca tagttcgacg acatgtccgg
     2281 attgccaaag tcgttttcaa tccaatgttc cggattactt tccaatgcaa taacatccgc
     2341 atttttaacg gcaataaaaa aactgtcggt aaggtgaaat gcaattttgt cgctcacatg
     2401 catcgtgccg tagatgtagg atggttcttt cagtccattt ccggtgattt cccacatgag
     2461 cgatgaattt tgtgcctgaa gcatacacat acatggcaca agtgcaataa cgagtgcaag
     2521 ttttctgagg tttctgtaca tagtttggtt taaggagtgt aaagcaatga tactaataaa
     2581 acatcaaatc cataagatat cgtataatca caacctattt ttacacaact catccacctg
     2641 tttttccaaa ataacggatg catcctacct ttgcagcatg gaaaccacca ttcgttacga
     2701 tttctccctc cttaccgaat atgacatcca tctgttcaaa caaggacgtc atttcagatt
     2761 gttcgaaaag ttgggtgctc atcctatgtc gggagaaggg caggaggggg tttactttgc
     2821 tgtatgggcg ccaaatgcaa aaggtgtttg cgttgcaggc acttttaatc actggaacac
     2881 cacagcatta ccattgcaat gtcgcaccga cggctcagga atttgggaag gtgttgtcag
     2941 taacgtaatg ggtggcgatc tgtataaatt tatcatcaac ggagccaatg gcgatgtttt
     3001 tgagaaggca gaccccttcg ctcgctatgc cgaaccagct ccgggaacag cttccattgt
     3061 ttgggacagc acttatacct gggaagacgc actctggcta accaaacgaa agcctttcac
     3121 cttcgacaag ccaatgagca tgtatgaggt gcatctgggt tcgtgggggc gacagccgga
     3181 tgatgccaat gcctttctta cgtatcgtca actcgccgaa acgcttgtgc catatgtcaa
     3241 ggaaatggga ttcacgcatg tagaactgct tccggtaaca gaacacccct tttacggctc
     3301 atggggatat caaacgctta gctttttcgc cccaacatcg cgctatggta caccgcagga
     3361 cttaatgtat ctgatcgata gcttccataa ggctcatatc ggcgttatcc tcgattgggt
     3421 gccctcacat tttccgcagg atggccatgg tttagcattt ttcgatggaa cccatctttt
     3481 tgaacatgaa gatcgcagga agggatttca ccccgactgg aactcataca ttttcaacta
     3541 tggacgtttc gaggtaaaaa gcttcctcat aagcagcgca ttattctggg tagatcaatt
     3601 tcactttgat ggtatcaggg tggacgccgt ggcaagtatg
//
LOCUS       DAOQHY010000259         3621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_16124_length_3621_cov_4.347729, whole genome shotgun sequence.
ACCESSION   DAOQHY010000259 DAOQHY010000000
VERSION     DAOQHY010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16124_length_3621_cov_4.347729"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PLL28_14180"
     CDS             complement(<1..458)
                     /locus_tag="PLL28_14180"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNF70525.1"
                     /translation="MQLVLVELINGKCKKTALEAVAFAAKMGETTALALGTASADTLQ
                     ALGAYGIGKVLHINNEKLNQVYSKAYTKAIAAAATHVGADTVILSHNSTGRMVAPRVA
                     VRMEAALFAGVNELPGNDTIRKAVFSGKAFADLQMSTGKKVISLNPNSYKA"
     gene            complement(481..1221)
                     /locus_tag="PLL28_14185"
     CDS             complement(481..1221)
                     /locus_tag="PLL28_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNF70526.1"
                     /translation="MKLLVCISKVPDTTAKVAFTDGNTRYNENGVQFIINPYDEWYAL
                     VRALELKETLGGSVTVINVGKADNEQIIRKALALGADDAVRIDMDPADAWQVASQIAQ
                     YAKGGGFDIIFTGKETIDYNGGIVGGMLAELLDLPYVALAAKLDVDGTQLNIAQEMEG
                     VKQLIKVSMPCVVSAQKGMAEARIPNMRGILASKSKPVQVIPASGDAAKSPVKVFVMP
                     AEKQGCKMISAENPEELVQLLHNEAKVI"
     gene            1351..1680
                     /locus_tag="PLL28_14190"
     CDS             1351..1680
                     /locus_tag="PLL28_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70527.1"
                     /translation="MERIPQLLSYLNDNPGDAFLNYALALEYIKIGDKEEGRKYFEHL
                     VNIQPDYVGTYYHLAKLYISMKLPEQAEQCFQSGIAVAQKLGDQHALAELQNAALNFR
                     LGLDDED"
     gene            1684..3045
                     /gene="tilS"
                     /locus_tag="PLL28_14195"
     CDS             1684..3045
                     /gene="tilS"
                     /locus_tag="PLL28_14195"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNF70528.1"
                     /translation="MDKLSTHQLHKFLHTLPGFTQQARILLAVSGGVDSVVMTYLLAS
                     ANYTIGIAHVNFQLRDGDAEKDEAFVKNLATSLNVPFFTTSVDTKKYASANKLSIEEA
                     ARNIRYQWLEEIRIQNNYDFIATAHHLNDHAETMLMHLTSGTGLQGMHGIRARNGNII
                     RPLLFATREQIETYASEHQLTFCTDVTNNSDVYLRNAFRKQVIPFLQQTNPSFLQTMA
                     TNAQHFREAHLLADERAVMLLHKWLKTTPTAISLPIKPITNHPAASTLLHTWLAPFGF
                     THAQITELATQQHVSGKQFLTNTHRLIVDRKHFLLTSYPQVNANIQIIENPSGSYHIK
                     NGKLKIEKMAMHPNINLQASNNIAYFDADKIEFPLTLRVWQKGDYMYPLGLFKKGKTK
                     PGKKKISDILTDARIAIPEKENTFVLCSGEYILWLIGIRSDERFRVSNNTTNVLKISM
                     LPD"
     gene            complement(3083..>3621)
                     /locus_tag="PLL28_14200"
     CDS             complement(3083..>3621)
                     /locus_tag="PLL28_14200"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70529.1"
                     /translation="INQFSNYLVFEWTPVTGATQYHLQLAYNASFSAIAKDVVTTNTF
                     YEATDLQMDKKYYWRVRPLAPANTCASPSNARSFTTGTELNSVANSEIFDHLAVYPNP
                     ALVGQNLTMEYRSAESFRADISLLDMTGRELTSTNYQINQGNNTLDVAVPAVSSGMYI
                     LRITAENRIETISVMIRN"
BASE COUNT         1089 a          772 c          733 g         1027 t
ORIGIN      
        1 gctttgtatg aatttgggtt tagcgatata acctttttac ccgttgacat ctgcagatcg
       61 gcaaaagctt tgcctgaaaa tacagctttg cggatggtat catttcccgg taattcattc
      121 acccccgcaa ataaagcagc ctccatacgc actgctacac gtggggcaac catacgtcct
      181 gttgagttgt gtgataaaat aacggtatca gcaccaacat gcgttgcggc cgctgctata
      241 gcctttgtgt aggctttaga atatacctga tttagttttt cattgttgat atgcaaaact
      301 ttgccgattc cgtaagcacc gagcgcctgc aaggtatctg ctgaggctgt tccaagggct
      361 aaggcagtgg tttcacccat tttggcagca aacgctactg cttccagggc tgttttttta
      421 cattttccgt tgataagttc tactaatacg agttgcatac gtcgttttct gataggtaaa
      481 ttaaattact ttagcctcat tgtgcagtaa ttgcaccaac tcttccggat tctcggcaga
      541 gatcatttta caaccctgct tctcggcagg catcacaaat actttcacag gagatttagc
      601 agcatcacct gaagcaggaa ttacctgcac gggttttgac ttggaggcca ggataccgcg
      661 catattcggg attcgagctt cagccattcc tttctgagca gaaacaacac aaggcataga
      721 cactttgatc agttgcttca ccccttccat ttcctgcgca atattgagct gagtgccatc
      781 aacatcaagt tttgcggcaa gcgctacata gggaaggtcg agtaattcgg ctaacatgcc
      841 gccaacaatt cctccgttgt aatcaattgt ctcttttccg gtgaagatga tgtcaaatcc
      901 gccacctttt gcatattgtg caatctgaga agctacttgc caggcatctg caggatccat
      961 atcgatacgt acggcatcat cggcacctaa tgccaacgct ttgcgaataa tttgttcatt
     1021 atcggccttg ccaacgttaa ttaccgtcac acttccacca agggtctctt tcaattcgag
     1081 ggcgcgaacc aaggcatacc attcatcata tggattgatg ataaattgca ccccgttttc
     1141 attatagcgg gtattgccat cggtaaaggc cacttttgcc gtagtgtcag ggaccttgct
     1201 gatacaaact aataatttca ttaggcttaa cttttgggac tgcaaagata ggtcataaaa
     1261 cccaaaatca tatatcatca ccaaccgcga attgtatggg ctgaaaggta tttctgtaag
     1321 gtagagtatc tttgacggca aggagtatac atggaaagaa taccacaatt attaagttat
     1381 ctaaacgata atcccggtga tgcatttctg aattatgcac tcgctcttga atacatcaaa
     1441 atcggagata aggaagaagg gcgtaaatat tttgagcatc ttgtaaatat tcaacccgac
     1501 tatgttggca cctattacca tcttgccaaa ttatacatca gtatgaaatt gcctgaacaa
     1561 gccgaacaat gttttcaatc cggcattgct gttgcacaaa aactaggaga tcaacatgca
     1621 ctggctgagt tgcaaaatgc agcattgaat ttccgtttgg gactggatga tgaagattaa
     1681 gtaatggata agctctctac acatcaactc cataaattcc tgcacacatt gccgggtttt
     1741 acgcaacaag ctagaatatt gcttgcggtg agtggtggtg ttgatagtgt ggtaatgact
     1801 tatttgcttg catcagctaa ttataccata ggtatagcac atgtcaattt tcagctgcgc
     1861 gatggagatg ctgaaaaaga cgaggccttt gttaaaaatt tagcgacctc gttaaatgtt
     1921 ccatttttca ccaccagtgt agatacaaaa aaatatgcaa gcgcaaacaa actatctatt
     1981 gaggaagcgg caagaaatat ccgctatcaa tggctggaag aaattcgaat tcaaaacaat
     2041 tatgatttca ttgcaaccgc acatcattta aatgatcatg ccgaaactat gcttatgcat
     2101 cttacttccg gaaccggatt gcagggaatg catggcatca gagcgcgcaa cggaaatatt
     2161 ataagaccct tattgtttgc aacgcgcgag caaattgaaa cctatgcttc agaacatcaa
     2221 ctgacattct gcaccgatgt caccaataac agcgatgtct atttgcgcaa tgcattccgc
     2281 aagcaggtaa ttccgttttt gcagcaaaca aatccatctt ttttgcaaac aatggcaacc
     2341 aatgcacaac attttcgtga ggcgcatctg cttgccgatg aaagggccgt aatgcttttg
     2401 cataaatggt tgaagacgac gccgactgcc atatcacttc ccataaagcc aataaccaat
     2461 caccctgctg cctctaccct actacataca tggttggcgc catttggatt tacgcatgca
     2521 cagatcactg aattggcgac tcaacaacat gtatcaggaa agcagttttt aactaataca
     2581 catagactga ttgtagatag aaaacatttt ttacttacaa gttatccgca agtaaacgcc
     2641 aacattcaaa ttattgaaaa tccttcagga agttatcata ttaaaaatgg taaattgaag
     2701 attgaaaaaa tggccatgca tccaaatatc aatctgcagg cttcaaataa tattgcctat
     2761 ttcgatgcag ataaaattga attcccgcta acattgcgag tatggcaaaa gggtgattat
     2821 atgtatccgc ttggcctatt caaaaaaggg aaaaccaaac cgggaaaaaa gaagatcagc
     2881 gatatattaa cggatgcacg catcgccatt cctgaaaagg aaaatacatt cgtattgtgc
     2941 agcggagaat atattctttg gttaattggt atccgcagcg atgagcgatt cagggtttcc
     3001 aacaacacca ccaatgtctt aaaaataagc atgctccccg attgaggagc atgcaacaaa
     3061 ataatctctt acgtaaatga tatcaatttc gtatcatcac actgatggtt tcaatgcgat
     3121 tttcggctgt aatacgaagt atatacatac ctgatgatac cgcaggaaca gcaacatcaa
     3181 gtgtgttatt accttgattt atttgatagt ttgttgaggt caattcgcgg cctgtcatat
     3241 cgagcaaact aatgtcggcg cggaatgatt cagctgaacg atattccatt gtaagatttt
     3301 gaccaactaa agcaggatta ggataaacag ccaggtgatc aaatatttca ctatttgcaa
     3361 cgctgtttaa ttcagtgcct gttgtaaatg atcttgcgtt agaaggagat gcacaagtat
     3421 tagccggtgc aagcgggcgt actctccaat agtatttttt atccatctgc aagtcggttg
     3481 cttcataaaa tgtattggta gtaacaacat ctttggcgat ggcactaaag cttgcattgt
     3541 aagccaattg caggtgatac tgtgttgcac cggtaacggg agtccattca aataccaaat
     3601 aattagaaaa ctggtttatg g
//
LOCUS       DAOQHY010000260         3586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_16388_length_3586_cov_5.312376, whole genome shotgun sequence.
ACCESSION   DAOQHY010000260 DAOQHY010000000
VERSION     DAOQHY010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16388_length_3586_cov_5.312376"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PLL28_14205"
     CDS             complement(<1..282)
                     /locus_tag="PLL28_14205"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HNF70530.1"
                     /translation="MKVLVVVFFVGVIAVLKLLFLQPLIYSVIVRIYGFGIRVASIFG
                     GKARAWVKGRENLFERLQQQVQSGGRKTIWMHCASLGEFEQGRPLLETLK"
     gene            complement(284..1036)
                     /locus_tag="PLL28_14210"
     CDS             complement(284..1036)
                     /locus_tag="PLL28_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70531.1"
                     /translation="MKSFSAICIGLLICSILNGQVNLKDSCVKANIIQVDLGVGMPAF
                     DMAERFGVHALVGGGYQYKSNHNWLFGVNGNFIYGSIVHEDSLMSNLRNEAGYVIGSD
                     GLGYNPILWESGFTAKWETGKITNIWSINPNSGLTFIGGIGLMQHKIWIYIDETVVPQ
                     LDETNRKGYDRLSNGLMLSQFAGYYLFSNKYFVNFRGGIEVVEAFTQNRRIVNYDTQM
                     TDDEMRFDMLINLKFSWNLPIYNQPKTRYYTN"
     gene            1155..>1900
                     /locus_tag="PLL28_14215"
     CDS             1155..>1900
                     /locus_tag="PLL28_14215"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNF70532.1"
                     /translation="MRKLQMVDLGTQYAAIRDEVNAGIQEVIDTTQFINGSATKAFAS
                     ELGQYLGVQHVIPCANGTDALQIALMAIGLEEGDEVITVPFTFVATVEVVALLKGKPV
                     FVDVDPLTFNMDARAIEAKITPRTKAIIPVHLFGQPADLSPIMELAEKHGIYVIEDNA
                     QAIGSNYTFPNGDTVKAGSIGHLATTSFYPTKNLGGYGDGGALFTNDDTLAQKIRIIC
                     DHGSSRKYYYDAIGVNSRLDSMQAAILRVKL"
     assembly_gap    1901..2000
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2106..3395
                     /locus_tag="PLL28_14220"
     CDS             2106..3395
                     /locus_tag="PLL28_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNF70533.1"
                     /translation="MIEQLRNKHARISVVGLGYVGLPIALEFGRHFHVTGFDIDPTRI
                     EALKNNVDPGGELPASYFENSTVDFTTDIDALKHCNFHIIAVPTPVDEHKVPDLSSLQ
                     SATQTIASVLKNGDYVVFESTVYPGCTEDICLPILESVSGLKVNTDFKLGYSPERINP
                     GDDVHTLRNVMKIVSGSDKEALETISDVYVSIVQPGVYQASSIKVAEAAKILENTQRD
                     LNIALMNELSIILDKMGVNTYEVIEAAGTKWNFHKYKPGLVGGHCIGVDPYYLTYKAM
                     ELDYEPYVILSGRKVNDNMAYHVAKRCVQKLIEAGKNLATSRILVMGITFKENVNDIR
                     NSKVADMIKELRSFGLQVDVVDPKADSAEVEKHYGFELQKVNGHQYDAIILAVPHKEY
                     VQLTPEYFASIATTDGIFIDIKGIFRDKINTLQYWSL"
     gene            3392..>3586
                     /locus_tag="PLL28_14225"
     CDS             3392..>3586
                     /locus_tag="PLL28_14225"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNF70534.1"
                     /translation="MNYQKTILVTGGAGFIGSHVVRLLVNKYPAYRIINLDKLTYAGN
                     LNNLSDVEHADNYAFVKGDIT"
BASE COUNT         1025 a          749 c          728 g          984 t
ORIGIN      
        1 tttcaaggtt tcaagtaatg gacgtccttg ctcgaattct ccgagtgagg cacagtgcat
       61 ccagattgtt tttcttcctc ctgattgtac ttgttgctgc aatcgctcaa aaagattttc
      121 tcttcccttc acccatgccc ttgccttacc accgaaaatt gacgccacgc gtatgccgaa
      181 gccatatatg cgtacgatta ccgaatatat gagcggctgt aaaaacaata gttttaatac
      241 ggcaatgacc ccaacaaaaa atacaacaac taatactttc atgtcaattc gtataatatc
      301 tagttttcgg ttgattgtaa attggcagat tccaactgaa ttttaaattg atcagcatat
      361 caaatcgcat ttcgtcatct gtcatttgtg tatcgtaatt cactatacgg cggttttgtg
      421 taaatgcctc cacaacttca atccccccac gaaaattgac gaaatattta ttgctgaata
      481 aataatatcc tgcaaactga ctcaacatca acccattcga caaacggtcg tagcctttgc
      541 gattggtttc atccaattgc ggcacaacag tttcatcaat ataaatccaa attttatgtt
      601 gcatcaagcc tattccgcca ataaacgtaa gcccggaatt cgggttgata ctccagatat
      661 tggtgatttt ccctgtttcc cattttgccg taaatccgct ttcccaaaga atgggattat
      721 atcccaggcc gtcgctgcca attacatacc ctgcctcatt gcgtaaatta ctcatcaatg
      781 aatcttcgtg cacaatgctg ccataaatga agttgccatt gacaccaaac agccagttgt
      841 gattggattt atactgataa ccgcctccca caagtgcatg gacaccaaaa cgttcagcca
      901 tgtcgaatgc cggcatgcca acgcccaggt ctacctgaat aatgtttgcc ttaacacagc
      961 tgtctttgag attcacctgc ccgttcaaaa ttgaacagat caggaggcct atgcaaatag
     1021 cggagaatga cttcataaat atgggcgtgc aaagttatta aaacttatgg tggtctgccc
     1081 taaaagaaca gacatccccg ataacgcctt ccaacgggtt tcagtatctt tgcaccctga
     1141 aatagggtag ggatatgaga aaattgcaaa tggttgacct tggtacgcaa tatgcggcta
     1201 tccgcgatga ggtgaacgcc gggattcagg aagtgatcga tacgactcaa ttcattaatg
     1261 gcagtgcgac aaaagctttt gcctctgaac tgggtcaata tctgggtgtg cagcacgtga
     1321 taccctgtgc caacgggact gatgcgcttc aaatagccct gatggccata ggattggaag
     1381 agggagatga ggtgatcaca gttcctttca cattcgtggc taccgtagag gtggtagcgc
     1441 ttttgaaagg aaaacccgtg tttgttgatg tggatcccct taccttcaat atggatgccc
     1501 gtgccataga agcaaagatc accccaagga ctaaagctat tatacctgtg catctgttcg
     1561 gtcagcctgc tgatctgtcg ccaataatgg aattggcaga aaagcatggc atttatgtaa
     1621 tcgaggataa tgcacaagcc atcggcagta actacacctt tccgaatggc gataccgtga
     1681 aagcgggtag tattggtcat ttggcaacaa catcgttcta tccgaccaaa aatctcggtg
     1741 gttacggcga tggtggcgct ttatttacca acgatgatac actggctcaa aaaattcgca
     1801 tcatctgcga ccatggatcc tcgcgtaaat attattatga tgctattggc gtgaattcac
     1861 gtctcgatag catgcaggct gcgattttac gtgttaaact nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn gaagtattat cgctgcccat gcattcggaa ttagatgccg
     2041 agcaaataaa ttttatatgt ggcgaaattg ccgctttcat gcaggcaaac gcttaaacca
     2101 tcgccatgat cgaacaactc aggaataaac acgcacgcat ctctgtagta ggtcttggct
     2161 atgtgggttt gccaatagcc ttagagtttg gcagacattt tcatgtcacc ggtttcgaca
     2221 tcgacccaac gcgcattgaa gcattaaaaa ataatgtaga tccgggtggc gaactaccgg
     2281 cctcctattt tgaaaattcc acagttgatt ttacaacaga cattgatgca ttaaagcatt
     2341 gcaattttca tatcatcgca gtgcctacac ctgttgatga acataaggtg cctgatcttt
     2401 ccagtcttca gtcggcaaca caaacaattg cttcagtatt aaaaaatggc gattatgtag
     2461 tatttgaatc tactgtttac cccggatgta cagaagatat ttgtttgccc attcttgaat
     2521 ctgtatccgg attaaaagta aatacagatt tcaaactcgg ttattctcct gaacgcatca
     2581 atccgggcga cgacgtgcac acattgcgca atgtgatgaa gattgtatcg ggctcggata
     2641 aagaagccct cgaaacaatt agtgatgtgt atgtttccat cgtgcaaccc ggtgtttatc
     2701 aggcatcatc tattaaggtt gcagaagctg ctaaaattct tgaaaatacg cagcgcgatc
     2761 tcaatattgc gctgatgaat gaactcagta tcattctcga caaaatgggc gtgaatactt
     2821 atgaagtgat agaagctgca ggcacgaaat ggaattttca taaatataaa ccgggacttg
     2881 ttggcgggca ttgcattggc gttgatccgt attacctcac ctataaggcc atggagcttg
     2941 attatgaacc ttatgtgatt ttgagcggca gaaaggtaaa tgacaatatg gcctatcatg
     3001 tggctaagcg ctgcgtgcaa aaactcatag aagcaggaaa aaatttggct acctccagaa
     3061 tattagtcat gggtatcacg ttcaaagaga atgtcaatga tatacgcaat tcaaaagtgg
     3121 ccgatatgat caaggaattg cgctcatttg gtttgcaagt tgatgtggta gatccgaaag
     3181 ccgatagtgc ggaagtggaa aagcattatg gatttgaact gcaaaaagta aatggtcatc
     3241 aatatgacgc cattatactt gctgtgcctc ataaagaata tgtgcaactc acacctgaat
     3301 attttgcatc tattgcaaca accgacggta tttttattga tatcaaaggt atattcagag
     3361 ataaaattaa tacccttcaa tactggagct tatgaattat caaaaaacta ttctagttac
     3421 cggcggcgcc ggatttattg gttcacatgt ggtgcgactg ctggtaaata aatatccggc
     3481 atatcgtatc atcaacctcg ataaattgac ctatgccggc aatttgaata atctctctga
     3541 tgttgaacat gccgataatt atgcatttgt aaaaggtgat atcaca
//
LOCUS       DAOQHY010000261         3585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_16401_length_3585_cov_4.207649, whole genome shotgun sequence.
ACCESSION   DAOQHY010000261 DAOQHY010000000
VERSION     DAOQHY010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16401_length_3585_cov_4.207649"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PLL28_14230"
     CDS             complement(<1..187)
                     /locus_tag="PLL28_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70535.1"
                     /translation="MSESELPEHKTGGFKILEIVPEIIAAFQIIISPLLIGSIIGAVI
                     YLTEPSPTRLLIGILIGI"
     gene            complement(298..867)
                     /locus_tag="PLL28_14235"
     CDS             complement(298..867)
                     /locus_tag="PLL28_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNF70536.1"
                     /translation="MRNLVFFMHASLDGFVGGPQGQLDWISVNDEIFDFVATMTDAAD
                     TALYGRVTYNMMESYWPTAGDKPNASKHDKEHAVWYNAVSKIVLSTTITAEGRKNTTV
                     ISDNITDAIQEIKAQEGKNILIFGSPSASHTLLNLGLIDEIWVFVYPVIIGSGIPLFK
                     DIQKKTALELVESKVFSNGGIALHYRIRK"
     gene            complement(991..>3585)
                     /locus_tag="PLL28_14240"
     CDS             complement(991..>3585)
                     /locus_tag="PLL28_14240"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNF70537.1"
                     /translation="RCGDLKYATYPVLVGHLKGDGIMSAESVVDHYMSNMLTKRLATG
                     NYPGDIGESKYFKNNPGMPKGTLIVGMGKTEKLSGFQLEETVKQGVIEYLCEFRVNEG
                     NVHGTVGLSSLLLGCGYAGLSVETAVKAVISGVHKANQSVRQNGDNIWPTVDEIEFIE
                     IYEDRALQALICLDNLKHTTNAGFNFEPTLYNLLGSRKRLLFEQNADWWQRVTIREQI
                     DCEQPSKGKIFTFTSSMGTARDEMRKLYCTKEVIDSLMKEISSRDVWDKELAKTLFEL
                     MIPNDFKLALRNQQNLLLIVDETAAAYPWEMLQDISIHSQPLATTAGMIRQLTTEDFR
                     PSVNYTLDNTALVIGNPNTANFLPALQGASDEAELVNNVLMDAGYQSTLSNNESSSVI
                     IRKLFRSDYKIIHLAGHGVYNHAQPDLSGMVIGNNVFLTTREIQQLSFIPELVFINCC
                     HLGNIEAMDEALYQNRYKLAANLGTQLINMGVKAVIAAGWAINDNAAKDFTEKFYAAM
                     LSGSNFGDAVRIARHYCYEHYPYSNTWGAYQCYGDQFFTLKRLNSGKKPSLRKYYVAN
                     QVRIDLENLINRIGANYSEIQKAAYLDEINLILDSAAAANIPQSSLYECLGIIYNRLG
                     NDSAAIENFKLYFEKSSETFNVSSYVEYARLLLSHYTHDKLAAKKIRAVVAELENLSK
                     TIPSYAIFNAIGTGYKILARMSKGADHSKNMLHAYRAYEHAYNSGKQAGVDNTMYALS
                     NAFMLGIALQDKKLQLPDISELEWSFNNIKAFDNTLTFWDLMDEPRYWLCKFVMQHQG
                     FVFPHGNVKVKAETVIDAWEKVVKTGGMETQQNEMIAKFASMVTFFDPKKFSKLRPKL
                     DQMMDALK"
BASE COUNT          998 a          749 c          675 g         1163 t
ORIGIN      
        1 atatgccaat caatataccg atgagcagtc tggtaggaga gggctctgtt aaataaatga
       61 ctgcgccaat tatcgagcca atcaatagcg gtgaaataat gatctgaaag gcagcaatga
      121 tttccggaac aatttctaat atcttaaaac ctccggtttt gtgttcgggt aattcacttt
      181 cagacatata aatgttttat atgtttacga atttgcatcg atgtatttac gtatgaatta
      241 ggcatattag aaaacaataa agcgagtcct attcgtaaac tttatttttt tgttgaatta
      301 ttttctaatt ctgtaatgca atgcgatacc accatttgaa aaaacctttg attcgactag
      361 ttcgagtgct gtttttttct gtatgtcttt aaacagtgga atgccgcttc caataataac
      421 cgggtaaacg aaaacccaaa tttcatctat aagtccgagg ttaagcagtg tatgtgatgc
      481 cgacggactg ccaaaaatga gtatgttttt accttcttga gcttttattt cctgaatagc
      541 atccgtgatg ttatcgctga ttactgtagt gttctttctt ccttcggcag taatggttgt
      601 tgacaacacg atttttgaaa ctgcattata ccaaacggcg tgttctttat cgtgtttaga
      661 tgcatttggt ttatcacctg cggtgggcca gtaactttcc atcatgttgt aagtaactct
      721 gccgtataat gcggtgtctg cagcatcagt catagttgcc acaaaatcaa aaatctcgtc
      781 gttgacgctg atccagtcta attgaccttg tggcccacca acaaatccat ctaaggaggc
      841 atgcataaaa aataccagat tccgcatgaa atgcttgttt agggcataaa tctagtgaac
      901 atcagcctca atgaaaagca gatgttaatt aatgcccgga ttaggtgcag gccatacttt
      961 ggattaatta ccgagttagc cctgatgcta tcacttcaaa gcgtccatca tctgatccag
     1021 ttttgggcgt aactttgaaa atttcttcgg atcaaaaaac gtaaccatcg aagcaaactt
     1081 agcgatcatt tcattttgtt gcgtttccat gccacctgtt tttacgacct tttcccaggc
     1141 atctattaca gtttccgctt tcaccttaac attgccatgt ggaaatacaa atccctgatg
     1201 ttgcataacg aatttgcaca gccaatagcg tggttcatcc attaaatccc aaaatgttaa
     1261 cgtgttgtcg aaagccttaa tattattgaa cgaccattct aattccgaaa tatccggaag
     1321 ttgaagcttt ttatcctgta aagcaattcc tagcataaag gcattactca atgcatacat
     1381 ggtattatct acaccggctt gtttaccact attataggca tgttcataag ctctataggc
     1441 atgcaacatg tttttactgt gatcggcacc tttgctcatg cgtgcaagaa tcttatatcc
     1501 tgtgccgatg gcgttaaaaa tagcataaga cggaattgtt ttagaaagat tctccagttc
     1561 tgccactaca gcacgtattt tcttggctgc tagcttgtcg tgcgtataat gcgacaataa
     1621 caatcgtgca tattctacat agctgctaac attaaatgtt tcactggact tttcaaagta
     1681 taatttgaaa ttttcaatgg ctgcactatc gttacctaat ctgttgtaaa tgatacccag
     1741 acattcatac agtgaagact gtggaatatt ggctgcggcg gcagagtcga gtatcagatt
     1801 gatttcatca aggtaagcag ccttttgtat ttcagaataa tttgcgccaa tgcgattgat
     1861 cagattttca agatcaattc tcacctgatt ggcaacataa tatttgcgca gcgatggttt
     1921 tttaccggag tttaagcgtt ttaatgtaaa aaattgatcg ccgtaacatt gataagcgcc
     1981 ccaggtattg ctgtaaggat aatgttcata gcaataatga cgtgctattc ttacggcatc
     2041 gccgaaatta ctgccggata acattgcagc atagaatttt tctgtgaaat ctttcgcagc
     2101 attatcatta attgcccatc ctgccgcaat aacggctttt acgcccatat taatgagttg
     2161 cgtgcctaaa ttagctgcca gtttgtacct gttttgatat aatgcttcat ccattgcttc
     2221 tatatttcct aaatgacagc aattgataaa gaccaattca ggaatgaaac ttagttgttg
     2281 aatctcacgt gtggtcaaga atacattgtt ccctattacc atacccgaaa gatccggttg
     2341 tgcatgattg tatactccgt gaccggcaag gtgaatgatc ttgtaatcgg aacggaataa
     2401 tttcctgata ataacgcttg atgattcatt attgctcaag gtagattgat aaccggcatc
     2461 catcaacaca ttgtttacca attcggcttc atcagatgcg ccttgaagtg ccggcaggaa
     2521 attagcagtg ttcggatttc caataaccag cgccgtgtta tctaaggtgt aattgacaga
     2581 aggtctgaaa tcttctgttg tcaattgcct tatcattcct gcggttgttg ccaaaggctg
     2641 tgaatgaatt gatatgtcct gcaacatttc ccatggataa gctgcagcag tttcatcaac
     2701 tatcaataac aagttttgtt gattgcgcag tgcgagttta aaatcgttgg gaatcatcaa
     2761 ttcgaacagt gtttttgcaa gctccttgtc ccacacatcg cgacttgata tttccttcat
     2821 caaagagtct attacttctt tcgtgcaata caatttgcgc atttcatctc gggcagtgcc
     2881 catggatgaa gtgaaagtaa atattttccc tttggatggt tgttcgcaat caatttgttc
     2941 gcgaatggta actcgctgcc accagtcggc attttgttca aacaacaatc gtttgcgact
     3001 gcccaataaa ttatataagg ttggttcgaa attaaaacct gcgtttgttg tatgctttaa
     3061 attatccaaa caaatcaatg cttgcagtgc tctgtcttcg tagatctcaa tgaattcaat
     3121 ttcatcaact gttggccaga tgttatcgcc attttgccgc acgctttggt ttgctttatg
     3181 cacgccggag atgactgctt taactgctgt ttctacgctc agccctgcat aaccgcaacc
     3241 taacagcaaa gaactcaacc caactgttcc gtgcacatta ccttcattta ccctgaattc
     3301 acagaggtat tctatcaccc cttgttttac tgtttcttca agctgaaatc cggatagttt
     3361 ttctgttttg cccatgccaa cgatcagtgt gcctttcggc ataccgggat tgttcttaaa
     3421 atatttactc tcacctatat cgccgggata attccctgtt gccaatcgtt ttgtaagcat
     3481 attcgacata taatgatcta ctactgattc agcactcata atgccatcac ctttgaggtg
     3541 accaaccagg acaggatagg ttgcatattt taaatcaccg catct
//
LOCUS       DAOQHY010000262         3578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_16444_length_3578_cov_5.454726, whole genome shotgun sequence.
ACCESSION   DAOQHY010000262 DAOQHY010000000
VERSION     DAOQHY010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16444_length_3578_cov_5.454726"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..>2303)
                     /locus_tag="PLL28_14245"
     CDS             complement(9..>2303)
                     /locus_tag="PLL28_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNF70538.1"
                     /translation="RKQFQRNHKLFDKDVETMPVFATIASQFNDPGTNQLYRHLMDTI
                     REKTGVDLHSDFHVTDEMSKKIFIIPPQRTRYLAEISENNRGYDKKASEQSKIAQQLY
                     ALHLSEQSIDDASVKHVIQESYQKLLKKLDHQHFDLIQQWDSKKKAYHDEFYIFKVRD
                     KELKIKTHSESLSHLQIPKISMPRYEAWGDILRWQLQENVPGEFPFTAGIYPFKREEE
                     DPTRMFAGEGGPERTNKRFHYVSLGMPAKRLSTAFDSVTLYGNDPEYRPDIYGKIGNS
                     GVSICCLDDAKKLYSGFDLADPKTSVSMTINGPAPMLLGFFMNAAIDQQCEKYIRANG
                     LEAEVEAKIKAIYAGKEQLRPKYNAAELPQGNDGLGLMLLGVTGDQVLPADVYADIKA
                     KTLSVVRGTVQADILKEDQAQNTCIFSTEFALRLMGDVQQFFISNKVRNFYSVSISGY
                     HIAEAGANPITQLAFTLANGFTYVEYYLSRGMHINDFGPNLSFFFSNGVDPEYAVIGR
                     VARRIWAKAMKHKYGADDRTQKLKYHIQTSGRSLHAQEIDFNDIRTTLQALYAIYDNC
                     NSLHTNAYDEAITTPTEESVRRAMAIQLIINKELGLAKNENPIQGSFIIEELTDLVEE
                     AVLSEFDRITERGGVLGAMETMYQRGKIQEESLYYETLKHTGEYPIIGVNTFLNSKGS
                     PTVIPMEVIRATEEEKEAQISTLRNLQSMQVEKAHHQLREIQLAAIENRNIFETLMEA
                     CKVCSLGQITKAMFEVGGQYRRNM"
     assembly_gap    2304..2403
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2404..3053)
                     /locus_tag="PLL28_14250"
     CDS             complement(<2404..3053)
                     /locus_tag="PLL28_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615065.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNF70539.1"
                     /translation="MTAASLFDGHDAAINVMRRIIQSTGAEVIHLGHDRSVEEVVNCA
                     IQEDVQAIAMTSYQGGHVEYFKYMYDLLKEKGASHIRIFGGGGGTILPTEIEELHDYG
                     ISRIYSPDDGRAMGLQGMINDLVKKSDIPTGVNLNGEINKLGIQDWGSIARLISAVEN
                     QTADAAGIMAQVEVMIKSSKTPVLGITGTGGAGKSSLVDELVRRFLLDFKDKTIAIL"
BASE COUNT          881 a          847 c          688 g         1062 t
ORIGIN      
        1 tccttttctc acatattccg tcgatactgc cctcccacct caaacatcgc cttcgtaatt
       61 tgtccaagtg aacatacttt acatgcctcc atcagcgtct cgaaaatatt tcgattttca
      121 atagccgcta attgaatttc acgcaattgg tgatgcgctt tttctacttg catggattgt
      181 aaattgcgca aggtggaaat ctgtgcttct ttttcttctt ctgttgcacg aataacttcc
      241 atcggtatca cagttggtga tccctttgaa ttgaggaaag tattcacacc aatgatcgga
      301 tattcaccgg tatgtttcaa cgtttcataa tataaactct cttcctgaat tttaccgcgc
      361 tgatacatcg tctccatagc gcccaacact ccaccgcgtt ctgtaatcct gtcgaattct
      421 gataatacgg cttcttcaac aagatctgtt aattcttcaa tgataaatga accctgaatc
      481 ggattttcat ttttcgcgag acctaattct ttattgataa ttaattgaat cgccatcgcc
      541 ctgcgcacac tttcttctgt tggtgtggtg atcgcttcat catatgcgtt ggtgtgcaac
      601 gaattacaat tatcataaat agcatacaat gcctgcagcg tagtgcggat atcattaaaa
      661 tctatctcct gtgcatgcaa gctgcgacct gatgtctgga tatgatattt taatttttgc
      721 gtgcgatcat cagcaccgta tttatgcttc atcgctttag cccaaatgcg tcgtgcaacg
      781 cgaccaatca ctgcatattc cggatccacg ccattgctga agaagaacga caaattcgga
      841 ccaaaatcat tgatatgcat gccgcgactc agataatact ctacatacgt aaatccattc
      901 gccaaggtaa atgccaattg cgtgatagga tttgcaccgg cttcggcaat gtgatatccg
      961 gaaatagata ccgaataaaa attgcgcact ttattgctga tgaaaaactg ctgcacatca
     1021 cccataaggc gtagtgcaaa ttctgtagag aaaatacaag tattttgtgc ctgatcttct
     1081 tttaaaatat cagcctgcac cgtaccgcga acaacgctca gtgtttttgc tttgatatca
     1141 gcatatacat ctgcaggcaa aacctgatcg ccggtaactc ctaaaagcat taaacctaaa
     1201 ccgtcatttc cttgtggtaa ttcagcggca ttgtattttg gacgtagctg ttctttgcct
     1261 gcataaattg ctttgatctt ggcttctact tcagcttcga gtccattagc gcgaatatat
     1321 ttttcacatt gttgatctat agccgcattc atgaaaaatc ctaataacat aggcgccgga
     1381 ccattaatgg tcatagaaac cgatgtctta gggtccgcca gatcaaatcc gctgtataat
     1441 tttttcgcgt catccagaca acaaatgctc acacccgagt taccgatctt tccataaatg
     1501 tccggacgat attcaggatc attgccatat aaagtaaccg agtcaaatgc ggtgctcagt
     1561 cgctttgcag gcatgcccag actcacatag tgaaagcgtt tgttcgtgcg ttcaggaccg
     1621 ccttctccgg caaacatacg cgtaggatcc tcttcttcac gtttgaatgg atagatgcct
     1681 gccgtaaatg gaaattctcc cggaacgttt tcttgtaatt gccaacgtaa aatatctccc
     1741 caagcctcgt accgcggcat actgatctta ggaatttgta aatgcgataa gctttcagaa
     1801 tgcgttttta ttttcaattc tttatcgcgc actttgaaga tataaaattc atcgtggtac
     1861 gcttttttct tgctgtccca ttgctgaatc aggtcgaaat gctgatgatc aagttttttt
     1921 aataatttct gataactttc ttgtatcaca tgtttaacgg aggcatcatc aatagattgc
     1981 tcacttaaat gcaaggcata taattgctgt gctattttcg attgttcaga ggctttttta
     2041 tcatatcctc ggttgttctc agaaatttct gcaagataac gtgtgcgctg tggaggaatg
     2101 ataaaaatct ttttactcat ctcatccgtt acatgaaaat cggaatgcag gtccactccc
     2161 gttttttcac gaatggtatc catgaggtga cgatacaact gattcgtgcc cggatcattg
     2221 aattgcgatg cgatggtagc aaataccggc atcgtttcta catccttatc aaataattta
     2281 tggttgcgtt ggaattgttt gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnagaatgg caattgtttt atccttgaaa tcgagtaaga atctgcgcac caattcatca
     2461 accaatgatg attttcctgc tccaccggtt cctgtaatac ctaaaaccgg cgttttgctg
     2521 cttttgatca tcacttccac ctgtgccatg ataccggcag catcagcagt ttgattttca
     2581 acagctgata tcaatcgggc gatactaccc caatcctgaa tacccagttt attgatctcg
     2641 ccattcaggt taacaccggt agggatatca ctttttttca ccaggtcatt gatcataccc
     2701 tgcaatccca tcgcgcgacc atcatcaggc gaataaatgc gcgaaatacc ataatcatgc
     2761 aattcctcga tttctgtcgg aagtatggta cctccgccgc cgccaaaaat gcggatatgc
     2821 gaagcacctt tttcctttaa caaatcatac atgtatttga aatactccac gtgtccacct
     2881 tgatacgagg tcatggcaat ggcctgcaca tcttcctgga tggcacaatt caccacctcc
     2941 tccacactcc ggtcgtgccc caggtgtatc acctccgcac cggtgctctg gatgatcctt
     3001 cgcatcacat taatggccgc atcatgcccg tcgaacaggc tggcagcggt cactatcctc
     3061 actttgtggc tgggttggta tggctgattg tccatcatat ggcagaatat acggcaaatt
     3121 tacggatatt accctgatgc tcgaaatggg caccaccctt gccgttctcc ccgggcggca
     3181 gtcctgcttt ccgctgcaag tcctccccct cgtatcctcg gtgtccgggc tttccgctac
     3241 aatcaggtgc agttaatacc cgtgtcccat ggccaaacct tgcagaaatg caattaagcg
     3301 cagaggccgc agattttcgc agagttggat acgcctcagc ccaaaactcc gctaactccg
     3361 cgatttaaac aggcgtgtga gtgagtctac ctcgccgtaa ggcggatggg tgaaattggt
     3421 gagtggtgag ttgtgagtgg tgaatttttg gattttgcat tccttttgca cttagcggtt
     3481 ttgcgtgcag ctgcattttg tgcatgtggt tcgtgtttta tgtcccacag attgcgcaga
     3541 gtttgatgca tccattccta aaactccgcc aactccgc
//
LOCUS       DAOQHY010000263         3537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_16764_length_3537_cov_6.110569, whole genome shotgun sequence.
ACCESSION   DAOQHY010000263 DAOQHY010000000
VERSION     DAOQHY010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16764_length_3537_cov_6.110569"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PLL28_14255"
     CDS             <1..334
                     /locus_tag="PLL28_14255"
                     /inference="COORDINATES: protein motif:HMM:NF025474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HNF70540.1"
                     /translation="NDTTQSYNLYINVRHTDAYPYNNIWINMTTTFPDGTKQSTKVDV
                     PLSEPEGEWMGNCVDGICFNTTLIKSDFFLPQKGKYTFTLEQDMRMNPVPDILDVGIR
                     VEKFMVTR"
     gene            406..2931
                     /locus_tag="PLL28_14260"
     CDS             406..2931
                     /locus_tag="PLL28_14260"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNF70541.1"
                     /translation="MHRQTLIAILLSISFGIRAQAPTQPYIIMITMDDMNAYLGAYGT
                     HPQVETPNLDALLSMGTIFTNAHCASPKCAPSRTSVITGKDVDYTQVYDNTSYACRDF
                     RKNFKVAYGNAEVFTLPEYLKDNGNYYTYTMGKTLHCYEAYSDYDTVTLDPCEKQFSW
                     NNAFVYVEDEIIDPIGDAENEGIFLLKWARLNDTMIDYMEDDVTVDHAISFINDFSTF
                     GTSITCDRPFFLALGIKKPHAPFYVPESFFNENYLNSVYDDPYDIPYNVPYNANPYNG
                     IVMPPQPEPRWADYDSLGYMGQLFASDNEEGQFEAFGESLPSLPEIQAGISDSERIEI
                     LNESMRANALMAYLAGVKFLDYEIGRFLDALESHPEIYNNTVIIITADHGFSHGQKRH
                     WGKFSLWEPDMRVPLVYIDLRNPVAQTCNASVSNMDIFPSVLDMLDMAEPTFLSGDRY
                     LDGYSFMPLINNPNQPWERPVLAGVRTKNSGGTLNDGSCFTQYSLRNNRFHYIKYATN
                     NAPPLLTCDFAASQTEEELYDVGVNRETDPNEWNNLVDDPEYAPVLNYMRQFIKDSTH
                     YKDRMFAVNINNTNTLPCFVKNTGKIKLQAALYDLNGALLTGAGYTFTWTNSLTAATV
                     AGKNYTFNMATVPPAVYAAHDHIMFYLKVTNTTTGELVAFNTRTFYINNANTPNAVYS
                     LVTDNIALTASITGYTLTGSYNNTYWNFGDGTQSEDFLPDTHYYAAPGFYTVRNYIQY
                     GNGCLKNVARNANLTREGVPQLQFALYPNPAKQSVTIALSEAITDADITVYNMLGQPV
                     YMQSIKEADRSFTIDITDIANGTYMVEISTPQFYGTKTLEVIR"
     gene            complement(2935..>3537)
                     /locus_tag="PLL28_14265"
     CDS             complement(2935..>3537)
                     /locus_tag="PLL28_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNF70542.1"
                     /translation="HYWHPAQKKGYSGVAILSKVKPDHVEIGCGNSAYDAEGRVIRAD
                     FGDKSVFSIYFPSGTTGDVRQQVKYQFLDYIYDYLMALNKKGRKLIVCGDYNIANKPI
                     DIHDPKGNKESSGFLPEERAWMDKFYENGFVDSFRQFNSEPHQYTWWSFRANARANNK
                     GWRIDYIAVSESLKKQLAGARIIPEAKHSDHCPQLLEVNW"
BASE COUNT         1043 a          708 c          714 g         1072 t
ORIGIN      
        1 caatgacact acgcaaagct ataatctcta tattaacgta aggcataccg atgcgtaccc
       61 ttacaataat atctggatca acatgactac cacctttcct gatggaacaa agcaatcaac
      121 aaaggtagat gtgcctttgt cggaacctga aggtgagtgg atgggaaatt gtgtcgacgg
      181 catttgtttt aataccacat tgattaaatc ggacttcttt ttaccgcaaa aagggaaata
      241 taccttcaca ttggagcaag acatgcgcat gaatccggtg cctgatattt tggatgtagg
      301 tatccgcgtg gagaagttca tggtgacaag atgatcacat tcgacggtcg aattattata
      361 atcacttagt aaattcctta ttttcgtatt tcaaatactc ccaacatgca caggcagaca
      421 ctcattgcaa ttttgctttc aatttcattt ggtattaggg ctcaggctcc cacccaacca
      481 tatatcatca tgattactat ggatgatatg aatgcatatc tgggtgctta cggaacgcat
      541 ccgcaggtgg aaacgccaaa tctggatgca ttgctgtcga tgggaacaat ttttacaaat
      601 gcgcattgtg cttcacctaa atgcgcccct tccagaactt ctgttatcac aggaaaggat
      661 gttgattata cgcaagtata tgacaacacc tcctatgcat gtcgcgattt ccgaaaaaat
      721 tttaaggtgg cttacggcaa tgctgaagta tttacattac cggagtatct gaaagataac
      781 gggaactatt atacctacac catgggtaaa accctgcatt gttatgaagc ttattccgac
      841 tacgataccg tgactttaga tccatgcgaa aagcaatttt catggaataa tgcattcgta
      901 tatgttgaag atgaaatcat agacccaatc ggagatgcag aaaatgaagg catcttttta
      961 ttaaaatggg cacgactcaa tgataccatg attgattata tggaagatga tgttacggtt
     1021 gatcatgcta tttctttcat taatgatttt tcaacttttg gtacatcaat tacatgcgac
     1081 aggccatttt tcctcgcctt gggaattaaa aaaccgcacg caccttttta tgtgccggag
     1141 agttttttca atgaaaatta tttaaatagt gtatatgacg atccatatga cattccatat
     1201 aatgttcctt ataacgctaa tccatacaat ggcatcgtaa tgcctcctca acctgaaccc
     1261 agatgggctg attatgattc attgggatat atggggcagt tatttgcttc tgataatgaa
     1321 gagggacaat ttgaggcctt cggcgaaagt ttgccttcat tgcctgaaat tcaagcaggt
     1381 atttctgatt cggaacgcat tgaaatatta aatgaatcaa tgcgcgcaaa tgctttgatg
     1441 gcttatctgg caggagtgaa attccttgat tatgaaattg gtagattttt agatgcattg
     1501 gaatcacatc ctgaaatata taataatact gttatcatca tcaccgccga tcatggtttt
     1561 agccacggtc agaaacgcca ttggggaaaa ttctctttat gggaacccga tatgcgtgtg
     1621 cctttggtgt atattgattt gagaaatccg gttgcacaaa cttgtaatgc atctgtgagc
     1681 aatatggata tctttccatc ggtattagat atgctggata tggctgaacc aacattttta
     1741 tccggcgacc gctatttaga tggatatagt ttcatgccat tgatcaacaa tccgaatcaa
     1801 ccttgggagc gtccggtact tgccggtgtg cgcacaaaaa atagcggagg cactttgaac
     1861 gatggctcat gctttacaca gtattctttg cgtaataatc gatttcatta tattaaatat
     1921 gccactaata acgcgcctcc gctgcttacc tgcgatttcg ctgctagtca aacagaggaa
     1981 gaactttatg atgtaggtgt taatcgtgaa acggatccca atgaatggaa taatctggtt
     2041 gatgatccgg aatacgctcc ggtattgaat tacatgcgtc aatttattaa agacagcaca
     2101 cattacaagg atcgcatgtt tgcagttaac ataaacaata caaatacact gccttgcttt
     2161 gtgaaaaata ccggtaagat taaattacag gcagcacttt atgatttgaa tggagcattg
     2221 ttgaccggcg caggctatac tttcacctgg actaatagtt taactgcagc taccgttgcg
     2281 ggaaaaaatt acacttttaa tatggcaaca gtgccgcccg cggtttatgc ggcacatgac
     2341 catatcatgt tctatctgaa agtaactaat actactacag gcgaattggt tgcatttaat
     2401 accagaacat tttatattaa taatgcaaat acccctaatg cggtttattc attggtaaca
     2461 gataatatcg cactgactgc ttctattaca ggttacacct taaccggtag ttacaataac
     2521 acctattgga atttcggcga tggaacgcaa tcggaggatt tcctgcctga cactcattat
     2581 tatgcagcac ccggatttta tactgtcaga aattatattc agtacggaaa tggatgtttg
     2641 aaaaatgttg cgcgcaatgc aaatctcact cgcgaaggtg taccccaatt gcaatttgcg
     2701 ctgtatccaa atccggcaaa acaatcagtt accattgcat taagtgaggc aataacagat
     2761 gccgatatca ccgtttataa tatgcttggt caaccagttt atatgcaatc aataaaagaa
     2821 gcagataggt catttactat cgatattacc gacatagcaa acggcaccta tatggttgag
     2881 attagtacac cacaatttta tggtaccaag actttggagg tgataaggta atttttacca
     2941 gtttacttcg agtaattgtg ggcagtgatc actgtgcttt gcttcgggta tgatgcgagc
     3001 acctgcaagt tgttttttaa gtgattccga aacagcaatg taatcgatgc gccagccttt
     3061 attatttgct cgtgcatttg cccgaaaact ccaccaggta tattgatgtg gctctgaatt
     3121 gaattgtcga aaactgtcga caaatccatt ttcatagaat ttatccatcc aggcacgttc
     3181 ctctggcaga aatcctgaag attctttgtt gcctttaggg tcatgaatgt cgattggctt
     3241 gtttgcaata ttgtaatcac cacaaacgat cagctttctt ccctttttgt ttaaggccat
     3301 gaggtagtcg tagatataat ctagaaactg atattttact tgctgacgaa catcgcctgt
     3361 ggtgccggat gggaagtaaa tggagaaaac gctcttatca ccaaaatcag ctcttatcac
     3421 gcgaccttct gcatcatatg cgctgttgcc gcatccaatt tctacgtgat caggttttac
     3481 cttgctgaga atggcaaccc cactataccc cttcttttgg gccggatgcc agtagtg
//
LOCUS       DAOQHY010000264         3532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_16818_length_3532_cov_4.460742, whole genome shotgun sequence.
ACCESSION   DAOQHY010000264 DAOQHY010000000
VERSION     DAOQHY010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16818_length_3532_cov_4.460742"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..896)
                     /locus_tag="PLL28_14270"
     CDS             complement(144..896)
                     /locus_tag="PLL28_14270"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70543.1"
                     /translation="MKKAFFLVSLVFTAILITESCKHTPVDLIDDLPGDPIIDTTGGP
                     TDTLVFSTCDSDTVYFENDIYPIFISNCAISGCHDAETHEEGLNLSTYTKIKTSGILN
                     LTHPWNSELIEVVTENDLDDRMPPWPMAALTDEEIQMLVTWMDQGALNNSCADCDTTA
                     ITFSGNILPIMDAYCTGCHDHTSPSGSIDLTAYNGTGSFDGISDVAADGRLVGAVTGA
                     AGYSFMPSGGMPLPDCLIEQIMTWVDEGYPDN"
     gene            1077..1769
                     /locus_tag="PLL28_14275"
     CDS             1077..1769
                     /locus_tag="PLL28_14275"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HNF70544.1"
                     /translation="MKKLHLLFLLPFAATTVFISCENLNLDDLLSNDDVVAGLKEALN
                     VGTDTAVAQGSVLNGYFLNPDITILFPEEAGIVQTVVEAIPGGSLLVDEFVESMNHAA
                     EDAATKATPIFKDAILNISFDDAMSILNGADTSATNYLRVNTFSDLYTAFKPDIQNSL
                     EGVGAQQAWEDVINLYNTIPFTEDVTTDLADYTTNKGLGGLFHLVGNEEIKIRTDIEH
                     QVTDLLQQVFGE"
     gene            1821..2456
                     /gene="pnuC"
                     /locus_tag="PLL28_14280"
     CDS             1821..2456
                     /gene="pnuC"
                     /locus_tag="PLL28_14280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HNF70545.1"
                     /translation="MQLSHCLHDSVLLMTIPEAIAVVTGVLSVWYARKESILVYPIGI
                     ISVLLYVYICLQVKLYADAAVNFYYFLASVYGWYFWLKGGRNQAESRGEEKAHIATFN
                     MPKQIGFALLTLLGGFVIGYLLDTYTDSNVPYWDGITTAIFCSAMWLMARKQIENWVY
                     WIIGDIICVPLFFSKGLCLSSLQYLIFTMLAVAGWITWNHRLKKRQEEQHA"
     gene            2449..2970
                     /locus_tag="PLL28_14285"
     CDS             2449..2970
                     /locus_tag="PLL28_14285"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF70546.1"
                     /translation="MRKIVFTGPECTGKTTLVNKLATELQLPYVPEVARGYIDQLQRA
                     YAYDDLLNIAMLQMSEEERVKSLQPHLLICDTDLLTVKIWLDDKFQSTESWLTHAFVD
                     RTPDMYFLCAPDFPWEPDAQREDPHRREYLHKIYRDHMLHYKLRFIELNGSIEKRLYT
                     VYNYINQPLHQLF"
BASE COUNT         1026 a          694 c          736 g         1076 t
ORIGIN      
        1 gtttacagga accgaaagca gcagcaataa tcaacccgcc ggtaaataac ccaattacgc
       61 ttttcataca attcaatctc ttttctgctt attacaacat caactgcgta ccattcattc
      121 cttgaaaatg atgattttac tcatcaatta tcaggatagc cttcatccac ccaggtcatg
      181 atctgctcaa tcaaacaatc aggcaatggc ataccgccgg aaggcatgaa tgaataacct
      241 gcagctcctg ttacggcacc tacaagtctg ccgtcagcag ctacatctga aatgccatca
      301 aaacttcctg ttccgttata ggcggtaaga tctatgcttc ccgaaggact ggtatggtcg
      361 tgacatccgg tgcaataggc atccatgatg ggtaaaatat tgccggagaa tgttatggca
      421 gttgtatcgc aatctgcaca actattattc aaagcaccct gatccatcca agtaaccaac
      481 atctgaattt cttcatctgt caaggcagcc ataggccaag gcggcatgcg gtcgtcaaga
      541 tcattttcgg tgacaacttc aataagttca ctattccaag gatgcgtgag gttaagaata
      601 ccgctggttt tgattttggt gtaagtgctc aggttaagac cttcttcatg tgtttccgca
      661 tcgtgacagc cacttatcgc acaatttgat atgaaaatag ggtagatatc gttctcaaaa
      721 tacacggtat ccgagtcgca cgtgctgaaa actaaggtgt cggttggccc tcccgtggta
      781 tcaatgatag ggtcaccggg cagatcatca atcaaatcta caggggtgtg cttacatgat
      841 tcagtgatca gaattgccgt gaacactagg gatacaagga aaaatgcttt cttcataata
      901 tcagagtagt aataatatat tacaaaatta actatgcaaa aagggtgcct catggacatt
      961 tatcacacaa ttccgacttt tttgaccttt tttctcttga atagttgcct ttttcgccca
     1021 catttgggga aaactggcac gattttttaa tttcgtatcg gtaaatcagc cattttatga
     1081 aaaaactgca tcttctcttc ctgttgcctt tcgccgcaac aactgttttc atctcttgcg
     1141 agaacctgaa tcttgatgat ctgctatcca atgacgacgt ggtagcggga ttgaaagagg
     1201 ctttgaatgt gggcacagat acagcggtgg cgcagggttc ggtgctgaat ggttattttc
     1261 ttaatccgga tattaccatt ctgtttcctg aggaagcggg catagtgcaa acagtggttg
     1321 aagcaatccc cggaggctct ttgctggttg atgaatttgt agaatcaatg aatcatgccg
     1381 ctgaagacgc cgctaccaag gccaccccga tttttaaaga tgccattctg aatatttcct
     1441 tcgatgacgc catgtctatc ctcaatggcg ctgacacttc tgctacaaac tatttaagag
     1501 tcaatacatt ctctgatctc tataccgctt tcaaacctga tattcaaaat tccttggaag
     1561 gcgttggtgc gcagcaggct tgggaggatg taatcaacct ttacaatact attccgttta
     1621 ccgaggatgt tactactgat cttgcagatt atactaccaa taaaggtttg ggtggcttat
     1681 tccatctggt aggcaacgaa gagattaaaa tccgtaccga tattgaacat caggtaacag
     1741 atttgttgca gcaggtgttt ggggaataat ttgtggtaaa ctatcattct ttgaataggt
     1801 agatttgcac aaatttcaag gtgcagttaa gccattgttt acatgattcc gtattgttga
     1861 tgacaatacc cgaagcgatc gcggtggtca caggggtgtt gagtgtctgg tatgccagaa
     1921 aagagtcgat tttggtgtat cctatcggta ttatcagtgt gctgttgtac gtatacattt
     1981 gtttgcaggt aaaactgtat gccgatgccg cagttaattt ttactatttc ctggcaagtg
     2041 tttacggatg gtacttctgg ctgaagggag ggcgtaatca agctgagagc aggggagaag
     2101 aaaaagcgca tatcgcaaca ttcaacatgc cgaaacaaat tggttttgca ctccttacac
     2161 ttctcggtgg atttgtaatc ggctacctac tggatacata taccgattct aatgtgcctt
     2221 attgggatgg aattaccaca gcaattttct gctctgcgat gtggctgatg gcgaggaagc
     2281 aaattgaaaa ctgggtatac tggatcatcg gcgatattat ttgtgtgcca ttattttttt
     2341 ctaagggatt gtgtctgagt tcactgcaat atcttatatt tacaatgctc gcagtggccg
     2401 gatggataac ttggaaccat cgcctgaaaa agcggcagga agagcagcat gcgtaagatc
     2461 gtatttaccg gacctgaatg cacgggtaaa actactctgg tcaataaatt agcaacagaa
     2521 ctacaattgc cctatgtgcc tgaagtggcg cgtggatata tcgatcagct gcaacgtgca
     2581 tatgcctatg atgatctgct caatatagcc atgttgcaga tgagtgagga agaaagagta
     2641 aaatctttgc aaccacatct gttgatttgc gataccgact tactcaccgt aaaaatttgg
     2701 ctcgacgata aatttcaaag cacagaatca tggttgacac atgcttttgt agacagaact
     2761 cctgatatgt attttttatg tgcgccggat tttccctggg aaccggatgc gcagcgtgag
     2821 gatccacatc gcagagaata tttgcataaa atatatcgcg accatatgtt gcactacaaa
     2881 ttgcgtttca ttgaactcaa cggctctata gagaagcgtt tgtataccgt ctataattat
     2941 atcaaccaac ctttgcatca gttgttttaa aatgtgtcgt ttattggcag aattactatt
     3001 gttgaattta ggtgggtaaa ttgacgcttt gattaattgg ttattatagc agaattcaag
     3061 ctttcgaagt ggtgcgatgg taacatatta taaaagtgtg agaaaatggg gactattttg
     3121 taccttgtaa agatttaatt gattacttac gggtttagct gaatttatca aaaaaatcca
     3181 tgatgtaaat caaatgtatt ttctttaatt atttctttaa tttcactcat catcttatta
     3241 aaatatacct tatgaaaact aataaatcaa attcaacaaa atcccattgc aatttacata
     3301 gtacttttga ctttcattgg ttcaaattta tttagtgcaa ttgtcaagaa cacagaatta
     3361 caccttaaaa atgaattgac ttttcaaaaa cccggaagaa caataatttg ttatccggat
     3421 actgttcctg tcgaactcga taccgataca gcttttgaaa taggagatac cattgacatt
     3481 aatttagcag gattagatac attattcata ctttatgaag acacaaattc ta
//
LOCUS       DAOQHY010000265         3461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_17407_length_3461_cov_5.492366, whole genome shotgun sequence.
ACCESSION   DAOQHY010000265 DAOQHY010000000
VERSION     DAOQHY010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17407_length_3461_cov_5.492366"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="PLL28_14290"
     CDS             complement(<1..739)
                     /locus_tag="PLL28_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70547.1"
                     /translation="MKKLLLIALFAACTGALSAQIDIGTTTYDVQTNNGAKHRVRVYD
                     DGSVSAIWTGSTDMGGTFPDRGMFFNHFDGLAWGAAPTLRAEAERTGFGELLTVDDHE
                     MIVAHNSGTNRMQIYANAAIGDNTWSEQPGSDQIRGIWPAAYCPEGTDDIYVVNADTQ
                     FVTGLNFSRSDDGGATWAVLNSALPFLTAADGMPAITNGAENYQIVAHGSDVYVLFGM
                     INSDLILMHSSEYGNEGTWEKITIIDFP"
     gene            928..1995
                     /locus_tag="PLL28_14295"
     CDS             928..1995
                     /locus_tag="PLL28_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HNF70548.1"
                     /translation="MALLYNRISHEELRQRMLASDEKRTTVSFYEYFPIADPQQFRDE
                     LYLKLQALGVMGRVYVAHEGMNAQISLPSDNFDAFAAFIRELVPSQQDNDPITGKPQL
                     RLNIAVDDNGKSFFVLKVKVRSKIVADGIDDPTFNMQNRGKYLKADEYNALAKDPETI
                     IVDMRNHYEYEVGHFERAIEVPSDTFREQLPMAADMLKDKKDANIIMYCTGGIRCEKA
                     SAYMKHVGFTNVFHIEGGIIHYANTVKEQQLENKFIGKNFVFDDRLGERISPEIIAKC
                     HQCGTPADTHTNCVNPACHLLFIQCEACAAKYERCCSVACQDTIHLPEEEQKRLRSGK
                     MKDQNIFNKSRSRMMDLERPQ"
     gene            2076..3329
                     /gene="metK"
                     /locus_tag="PLL28_14300"
     CDS             2076..3329
                     /gene="metK"
                     /locus_tag="PLL28_14300"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNF70549.1"
                     /translation="MPYLFTSESVSEGHPDKIADQISDALIDHFLAFDPNSKVACETL
                     VTTGQVVLAGEVKSKTYLDVQDIAREVIRNIGYTKSEYMFEANSCGILSAIHEQSGDI
                     NQGVERKKKIEQGAGDQGMMFGYACRETDNYMPLSLEIANLLLKELAAIRREGKVMTY
                     LRPDAKSQVTIEYNDDNTPMRIDAIVVSTQHDDFDEEKKMLASIKTDIQNILIPRTIK
                     KLPKRLHKLFDGKYKLFVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSG
                     KDPSKVDRSAAYAVRHIAKNMVAAGICDEVLVQVAYAIGVAEPVGLYVNTYGTAKVKM
                     QDGEIAKRIRKIFDLRPYAIEQRFQLRTPIYLETAAYGHMGREPKTITKRFVDGGGRK
                     KSIRVTLFPWEKLDYVDAIRKAFKL"
BASE COUNT         1032 a          705 c          801 g          923 t
ORIGIN      
        1 aaggaaaatc tatgattgta attttctccc aggtgccttc atttccatat tcagatgagt
       61 gcattaatat caggtcgctg ttgatcattc cgaacaacac atatacatcg ctgccatgtg
      121 caactatttg gtaattttca gcaccattag taatcgcagg catgccatcg gcagcagtaa
      181 gaaatggtaa agcggaattc aatacagccc aggtagcgcc gccatcatcg cttcgcgaaa
      241 agttaaggcc tgtaacaaat tgagtatcgg cattaacaac ataaatatca tcagttcctt
      301 caggacaata tgctgccggc caaatgcctc ttatttgatc ggaaccaggt tgttctgacc
      361 aggtattatc gcctatggca gcatttgcat aaatctgcat acggttagtt cccgagttgt
      421 gggccactat catttcatgg tcgtcaactg tcaggagttc tccaaatcct gttctttcag
      481 cttcggcacg caatgtaggt gcagctcccc aggcaagtcc gtcgaaatgg ttaaagaaca
      541 tgcccctgtc gggaaatgta ccacccatat cggtagaacc tgtccaaatg gctgacacgg
      601 agccatcatc gtatacgcgt acacggtgtt tggcgccatt gtttgtttga acatcatagg
      661 tagtggtgcc gatgtcgatt tgggcgctca aggcacctgt acaagcggcg aaaagtgcaa
      721 ttagtaggag ttttttcatg atgtgttggt tttgattggc aatttgtgac tgtaagttta
      781 cgaaatctac aaatgaatat ttaaagtttt aacatcaccc tcttttgtca ttgagaaaag
      841 tcaccatgca gtaagcgtgc aaaagcggta tctttgcact gtttactgaa gtttcacccc
      901 gcctgcaacg ggatacaaaa tttcgacatg gcacttttat acaacagaat atcccacgag
      961 gaattacgtc agcgtatgct ggcatcagat gaaaaacgga ctaccgtttc attttacgaa
     1021 tacttcccta tcgccgatcc acagcagttt agagacgagc tctatctaaa gcttcaggcc
     1081 ttaggtgtga tgggacgcgt atatgtggca catgaaggaa tgaatgctca gatcagtctg
     1141 cccagcgaca atttcgatgc ctttgctgca tttattcgtg agcttgtgcc ttcgcaacag
     1201 gataatgatc ctataacagg aaaacctcaa ttgcgattaa acattgctgt agacgacaat
     1261 ggaaaatcat tttttgtatt gaaggttaaa gtgcgttcaa aaattgttgc cgatggcatt
     1321 gatgacccca catttaatat gcaaaatcgc ggtaagtatc tgaaagctga tgagtataat
     1381 gcactggcga aggatcctga aaccattatt gtagatatgc gcaatcatta tgagtatgag
     1441 gttggtcatt tcgaacgtgc gatagaggtt cccagcgata ccttcagaga gcaactgcca
     1501 atggctgccg acatgttaaa ggataaaaag gatgccaata tcatcatgta ttgcacaggt
     1561 ggtattcgtt gtgaaaaggc aagtgcatac atgaaacatg taggatttac gaatgtgttt
     1621 catattgagg gaggcattat tcactatgcc aatacggtaa aagagcaaca acttgaaaat
     1681 aaattcatag gcaagaactt tgtgttcgac gacaggcttg gcgaacgcat ttcaccggag
     1741 atcattgcca aatgccatca atgcggcaca cctgccgata ctcacaccaa ctgtgtgaat
     1801 ccggcatgcc acctcctatt cattcaatgt gaggcttgtg ctgccaaata tgagcgctgt
     1861 tgttctgtag cctgccagga taccatacat ctacccgaag aggagcaaaa acgcctccgc
     1921 agcggcaaga tgaaagatca aaatatcttt aataagtcga gaagtcgcat gatggacctt
     1981 gagcgaccgc aataaggctg caatttccac attccgggtt agtacatata ggggcaagaa
     2041 gttatctttg cacctacaaa aaactataag cttaaatgcc ttatttattt acgtccgaaa
     2101 gtgtgagcga aggccaccca gataagatcg ctgatcagat atcagatgcg ttgatcgatc
     2161 attttttagc ctttgacccg aacagcaagg ttgcttgtga aacactggtt actacagggc
     2221 aggttgtact tgccggtgaa gtaaaatcaa aaacatatct cgatgtgcag gatattgcgc
     2281 gcgaagtaat tcgcaatatc ggttacacaa aaagtgaata tatgtttgaa gctaattcat
     2341 gtggtatctt aagtgccatc catgaacaga gcggcgatat caatcaaggt gttgaacgaa
     2401 aaaagaaaat tgaacaaggc gccggtgatc aggggatgat gtttggatat gcttgtcgcg
     2461 aaaccgataa ttacatgcca ttatcacttg aaattgcaaa tttattattg aaagaactcg
     2521 ctgccattcg tcgcgaagga aaagtgatga cataccttcg tccggatgca aaaagtcagg
     2581 taacgattga atacaatgac gacaatacgc caatgcgcat agatgcaatt gtagtatcta
     2641 cacagcatga tgattttgat gaagagaaaa aaatgttggc gtcgataaaa actgatattc
     2701 aaaatattct gattcctcgc accattaaaa aattacctaa acgtttacat aaactgttcg
     2761 acggaaaata taaattattc gttaacccaa caggtaaatt tgtaatcggc ggaccacatg
     2821 gtgataccgg acttaccggt cgtaaaatca ttgttgacac ctatggtggt aaaggcgcgc
     2881 atggtggtgg tgcattcagt ggaaaagatc cttcgaaagt tgaccgctcc gcagcatacg
     2941 ccgttagaca tattgcaaaa aacatggtag ccgccggtat ttgcgatgaa gttttagtgc
     3001 aggttgccta tgccattggt gtagctgagc cggtaggctt atatgtaaat acttatggca
     3061 ctgcgaaagt taagatgcaa gacggagaaa ttgcaaaacg cattcgcaaa atatttgatc
     3121 ttcgtccata cgctattgaa caacgctttc aattgcgcac gccaatatat ttagaaactg
     3181 cagcctatgg tcacatgggt cgcgaaccca aaaccatcac gaagcgattt gttgacggtg
     3241 gaggaagaaa gaaaagcatt cgcgtaacct tattcccttg ggagaaattg gattatgtag
     3301 atgcgatcag gaaggcgttt aagttgtgag tgggtgagtg gtgaatggtg tggtgggtcc
     3361 agatccctcc gcaaactccg ccaactccgc gattaaaata tggtgagtgg gtgactggtg
     3421 agcttgcctc gccgataggc gggtggtgag tcgtgaatgg t
//
LOCUS       DAOQHY010000266         3434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_17618_length_3434_cov_5.395975, whole genome shotgun sequence.
ACCESSION   DAOQHY010000266 DAOQHY010000000
VERSION     DAOQHY010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17618_length_3434_cov_5.395975"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="PLL28_14305"
     CDS             complement(<1..939)
                     /locus_tag="PLL28_14305"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HNF70550.1"
                     /translation="METIIPIGYWIFFIFTIVGTLAGLYKMFEKAGEAGWKALVPFLN
                     AWICVKITGKSRSWFIMLMIPVLNVVVWLLMSNELAKVFGKESFWAYVGSMMIPYIYF
                     LKLGFDEDVRYIGPHKSERKSVGREWADAIAFAVVAATLIRTFFFEAYTIPTTSMEGT
                     LKAGDFLFVSKFHYGARFPITPLAFPFAHHTMPVIGGKAYSDAIKMPYMRFPGLENIH
                     RGSSVVFNFPAGDTVYLPEQQNSYYSLLMNEATNLQINDAKLGNRPKPFSFYYNRVSA
                     AYRSSGELAYRPVDKRDNYIKRCVGLPGDSLWVSNGI"
     gene            complement(1034..2506)
                     /locus_tag="PLL28_14310"
     CDS             complement(1034..2506)
                     /locus_tag="PLL28_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70551.1"
                     /translation="MSHSKGNIEGIDGKKGRDIVTLLQEPQVWIRAGVLFCLAFGVMT
                     MNLPFYGDQVTLVSAPASYIYEHGIHSPILPDDLNTGHFPLFPLLTAGFWVVFGRNLI
                     SVHLLMILCQLFMFSQVISFARVKLQKATLAVFTLLLLCYPVWWAQTAGMSADIMLCG
                     LFFMGLRAGISKKWVVWMIILGLLPLVSLRGWIWAFAMCIYVWSGEQFRFRWFMMIAL
                     RTLIALTPIIAYYTWQHAVSRWWIVPIDERYGEHRQLVDGKLLAGKGFEFLLRCIEFG
                     MVIPVVVMIMRGSALLNRFSITSNIGLIAISVFSIALFTLPFRGPILIRYMMPLHVLI
                     LIASAPFFVKAGGQLFRGGLVAMTACVFIAQHFIAYPQMRTSIFEYDWSGGSLSHLSY
                     FRFRAEGETYLKAHHIDDSEVGTYFPEYKSFALTDLVEDDTEYNAISPDSIPTSELLI
                     YSNVMNSVTMPMYDSIKAHYTLDKRWFSYPVTYEIYRRIP"
     gene            2549..>3434
                     /locus_tag="PLL28_14315"
     CDS             2549..>3434
                     /locus_tag="PLL28_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNF70552.1"
                     /translation="MYDNNPTLEGLDQHKLLDETRQGEALGNEAMAEAATGKRFYIES
                     YGCQMNFSDSEIVASILHQHGFGLTNDYNDADLILLNTCAIRDNAEQRVRNRLKELGA
                     VKKRKPEALIGMLGCMAERLKADLLEEAKLVDIVCGPDAYRDLPMLVNTADDGAKAVN
                     VLLSREETYSDISPLRLDANGVCAFISIMRGCDNMCSFCVVPFTRGRERSRNAFSILA
                     EARDLFERGYKEVTLLGQNVDSYKWENPETGKVVNFAELMAMVASVDPSLRVRFSTSH
                     PKDITDEVLHTMARFDNIC"
BASE COUNT         1075 a          783 c          701 g          875 t
ORIGIN      
        1 aattccattg ctcacccaca aagaatctcc cggaagtccc acacaacgct tgatataatt
       61 gtcgcgcttg tcaacagggc ggtaagcaag ctctcctgaa ctgcgatatg ctgccgatac
      121 tctgttgtaa taaaatgaaa acggttttgg acgatttcct agttttgcgt cattaatttg
      181 aaggttagta gcttcattca tgagcagtga atagtaactg ttttgctgtt caggcaaata
      241 cactgtatca cccgcgggaa aattaaaaac tacactgctt ccgcgatgaa tattttcaag
      301 cccgggaaat cgcatatatg gcatcttgat ggcatcagaa tatgctttac ctccgatgac
      361 aggcattgta tgatgtgcaa atggaaatgc caatggagtg atcgggaaac gtgcaccgta
      421 atgaaattta cttacaaata agaaatcacc tgcctttaaa gtgccttcca ttgaagttgt
      481 cggaatagta taagcttcaa agaaaaatgt acgtatcaag gtagcagcta ctacagcaaa
      541 agctattgca tctgcccatt cacgtccaac tgattttcgc tccgatttat gaggtccgat
      601 gtaacgcaca tcttcatcaa atcccaactt gagaaaatag atgtatggaa tcatcattga
      661 tccaacatat gcccaaaaac tctccttgcc aaaaactttc gccagctcat ttgacatgag
      721 taaccatacc accacattca aaaccggtat catcagcatg atgaaccacg atctgctctt
      781 accggtaatc tttacgcata tccaggcatt taaaaaagga actaaagcct tccaaccggc
      841 ttcacctgct ttctcaaaca ttttatacaa gcccgcaagt gtgccaacta ttgtaaaaat
      901 gaaaaatatc caataaccaa taggaatgat ggtctccatg cacgtatttt attgtaaagt
      961 aaatgactaa gcttgctgca aagctagaag atttcgatga atagcggttt aatggcggta
     1021 aacctcccta acatcagggg attcttctgt atatctcata agtaacagga taactgaacc
     1081 atcttttgtc caatgtataa tgtgctttta tactgtcata catgggcata gtgacactgt
     1141 tcatcacatt tgaatatatc aatagctctg aagttgggat tgagtcggga gaaatggcat
     1201 tgtattcagt atcgtcttca actaaatctg tcaaggcaaa ggatttgtat tcaggaaaat
     1261 atgtgcctac ttcgctgtcg tcaatatgat gtgccttcag atatgtctca ccctccgctc
     1321 tgaatcggaa ataactcaaa tgcgataagg acccgccgga ccaatcatat tcaaaaatag
     1381 atgtgcgcat ttgcggataa gcgataaaat gctgtgcaat aaatacacat gcagtcatcg
     1441 caacaagacc tccccggaat aattgacctc ccgctttaac aaaaaatggt gcagatgcta
     1501 tgagtattaa cacatgcaaa ggcatcatat acctgattaa aatgggaccc cgaaagggta
     1561 gtgtaaatag ggcaatactg aaaaccgaaa tagcaattaa tccgatgttg ctcgtaattg
     1621 aaaatcgatt aagcaaagcg ctcccacgca taatcatgac taccaccgga atcaccattc
     1681 cgaattcaat acaccttagc aagaactcaa atccttttcc tgccaatagt tttccatcaa
     1741 ccagttgcct atgttcgcca taacgctcat ctataggcac tatccaccac ctgctgactg
     1801 catgctgcca tgtgtaataa gcaatgatag gagtcaaggc aatcagcgtc ctcaaagcga
     1861 tcatcataaa ccacctaaaa cggaactgct ctcccgacca tacataaata cacattgcga
     1921 aagcccaaat ccacccacgc aaacttacca gtggtaataa gcctaaaata atcatccaca
     1981 ccacccactt tttgctaatt cccgctctta aacccatgaa aaacaagcca cagagcataa
     2041 tatcagcact cattccggcc gtttgcgccc accaaaccgg ataacaaagc aacaaaagtg
     2101 tgaaaaccgc caaagtagcc ttttgcaatt tcacccgtgc gaagcttatc acctgcgaaa
     2161 acataaacaa ctggcaaagt atcatcagca ggtgaacgga gatgagattt ctaccaaaaa
     2221 ctacccaaaa acctgcagtc aggagaggaa acaaagggaa atggcccgta tttaggtcat
     2281 ccggaaggat aggggagtgt ataccatgtt catagatata ggaggccgga gcactaacaa
     2341 gtgtcacctg atcgccataa aagggcaaat tcatggtcat taccccaaaa gcaaggcaaa
     2401 acaagacacc cgctcgtatc catacctgtg gctcctgaag caaggttaca atatctcgac
     2461 ccttttttcc gtctatgccc tctatattgc ccttggaatg tgacaagttg tcagttttta
     2521 cgtaaatttg catcaaactt aagcagaaat gtacgataat aatcctaccc tcgaaggttt
     2581 ggaccaacat aaactgctcg atgaaacgcg acagggggaa gcattgggca acgaagcaat
     2641 ggctgaagcg gcaaccggaa agcgttttta catcgaatct tacggatgtc agatgaactt
     2701 cagtgattct gagattgtag cctcaatatt acatcaacac ggtttcggac tgacaaacga
     2761 ttataacgat gccgacctga tattactgaa tacttgtgcc attcgcgaca atgcagaaca
     2821 gcgtgtgcgc aacaggttga aggagttggg agcggtgaaa aaacgcaaac cggaggcgtt
     2881 gattggaatg ctcggttgca tggcagagcg actaaaagcc gacctgctgg aagaggctaa
     2941 actggtggac atagtttgtg gtcctgatgc gtatcgcgac ctccctatgc ttgtgaatac
     3001 agccgatgat ggcgctaagg cagtaaatgt gttgctcagt cgcgaagaaa cttacagcga
     3061 tatcagtccg ttgcggctcg atgccaatgg cgtgtgtgct tttatttcta ttatgcgtgg
     3121 ctgtgacaat atgtgttcat tctgtgttgt accctttacg cgcggacgcg aacgcagtag
     3181 aaatgccttt tcaatactag ccgaagcccg tgatctcttt gaaagagggt acaaggaagt
     3241 aaccctcctt gggcagaatg tcgattcgta taaatgggaa aatcccgaaa caggaaaagt
     3301 ggtgaatttt gccgaattga tggcaatggt agcctctgtt gacccttcac taagagtgcg
     3361 tttttcgact tcacatccga aggatattac tgatgaggtg cttcatacca tggcgcgatt
     3421 cgataatatc tgta
//
LOCUS       DAOQHY010000267         3413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_17778_length_3413_cov_16.943419, whole genome shotgun
            sequence.
ACCESSION   DAOQHY010000267 DAOQHY010000000
VERSION     DAOQHY010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17778_length_3413_cov_16.943419"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PLL28_14320"
     CDS             complement(<1..222)
                     /locus_tag="PLL28_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70553.1"
                     /translation="MENRKFSLKQYSGFAGVLLLMQNNTHAQAIYTDIDPDVVLQFNG
                     DVEYIDMDNDGTNDFFIFKKSSYFTNSEYT"
     gene            complement(222..818)
                     /locus_tag="PLL28_14325"
     CDS             complement(222..818)
                     /locus_tag="PLL28_14325"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70554.1"
                     /translation="MKSETQSYVVKKGYLLGLYPGSTALAYGEALLYNIDALQLYSNS
                     GNSYLYKLNICYNNTDQLHPFEIQGINGPTANVPECSGLADGAICVTVPATSFGYIEI
                     PFYVSAREGIILSDDQVELFPNPTTNAFRITCTIPEELLNEFVVDVFDLQGKLMMHAA
                     TSQNKDIDVSNLPSDIYVVKISNREQSFTVTKKLIKIE"
     gene            1010..2551
                     /gene="istA"
                     /locus_tag="PLL28_14330"
     CDS             1010..2551
                     /gene="istA"
                     /locus_tag="PLL28_14330"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HNF70555.1"
                     /translation="MANKSISMSKVRQVLKLYAQKIGKRKIGLRLGMSKNTVRHYIEA
                     FHGMKTTIEELSKLSDADLNKRFHPPQEAIVSDKLAELHAFFPEMQKQLRKRGMTIAK
                     QYRTFAKTHPDTYKETAFYKYYRLWCKRVNPSMHIEHIAGDKLYVDYAGGTLPYVDTD
                     TGEVKEAQVFVAILGWSQFAFVEAMQNQTVPEFIAGCEHAMQYCGGVPLALVPDNLKS
                     AVFKAHKYEPILNENFRSFAAHYGCTILPARARKPQDKAHVENMVKIVYQNIYTVMPQ
                     GELLSLSQLNQAIRKQLSLLNAQLLTDKNCSRSDLLAMEQSALHPLPDTIYEIRKIKQ
                     VTVMKNYHVLLTEDHHYYSVPYDLIGKKLSMQYTRSVVELYLNYELVATHQRVRSPHN
                     YTTEPAHMPATHQFVHEWSPGFFLEKARAIDPVVEEYIGHVLAKKQHPEQAYKSCQGI
                     LSFAKRVGHQRLINACKRGLDIGYYNYRLIDDILKKNMDAYEDEKPAEHMPSHENIRG
                     GNYYQ"
     gene            2593..3327
                     /gene="istB"
                     /locus_tag="PLL28_14335"
     CDS             2593..3327
                     /gene="istB"
                     /locus_tag="PLL28_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HNF70556.1"
                     /translation="MTETVAKMKQMRLLGMARAFTHTMESGKNESFTPDEMITHLIDS
                     EWDERYNRKLDRTVKEARFRYKASIEQIGFEDNRVDKNQILRLADCSFIKRAENIIIT
                     GSTGIGKSFLASAIGHQACSLGYKVLYQHSTKLFARLKIAKADGSFLKELSKIEKQHL
                     LLIDDFGIQPLDAQSRSTLMEIIEDRHGKSSVIITSQVPVNQWHDIIGEQTIADAILD
                     RIVHDAHRINMKGESLRKKRDLQNEE"
BASE COUNT         1076 a          734 c          716 g          887 t
ORIGIN      
        1 ggtatattca gagttggtaa aatagctact ctttttaaat ataaaaaaat catttgtccc
       61 gtcgttatcc atatcaatat attcaacatc cccattgaat tgtaaaacaa catcagggtc
      121 aatgtccgta tagatagctt gtgcatgtgt attattttgc ataagtaaca ataccccggc
      181 aaatccggag tattgtttta aactaaattt tctgttttcc attattcaat ttttatcagt
      241 tttttagtta cagtaaagct ttgttcacgg ttgctaattt taactacata aatatctgaa
      301 ggtaaattac ttacatcaat gtctttattt tgacttgttg cggcatgcat cattaatttg
      361 ccttgcaaat cgaacacgtc aaccacaaat tcattaagca gctcctcagg aattgtacag
      421 gtaatgcgaa atgcattagt agtcgggttt gggaataatt ctacttggtc gtcggataaa
      481 attatgcctt cgcgagcgga tacgtaaaat ggaatttcaa tatatccgaa tgaagttgcc
      541 ggcacagtta cacagatggc tccatctgct aaaccggaac attcgggcac attagccgta
      601 ggaccattaa tgccttgaat ttcaaaagga tgtaattggt cggtattgtt gtaacaaata
      661 ttcaacttat ataaatagct atttccacta ttggaataca actgaagagc atcaatattg
      721 tataacaagg cctctccgta ggcaagcgcc gttgagcctg gatacagacc aaggagatag
      781 ccttttttta ctacataact ttgagtttca gatttcatgt ttgaatggaa attccgcgca
      841 aacagacccc acttgaccgc gtgaaattga ccccacttct gcgcatgtaa ttgaccccac
      901 ttctgcgcgg caaacagacc ccaccttttt tgatttgatc atactgtttt agcatactga
      961 ttttggtttg cagagtcgta atatccgctt ctgtaaactt aataaaatca tggctaataa
     1021 atcaatcagt atgagtaagg taagacaagt attaaagctg tatgcccaaa agataggaaa
     1081 aaggaagatc ggtctacgac tgggcatgtc gaagaatacg gttaggcact atatagaagc
     1141 cttccatgga atgaaaacca cgatcgagga gctttccaag ctttcggatg cagatttgaa
     1201 taaacgcttc catccacctc aggaggcgat tgtgagcgac aagctggctg aactgcatgc
     1261 tttctttcct gagatgcaaa agcagctgcg caagcgtggg atgaccattg cgaagcaata
     1321 tcggacgttt gctaaaacac atcctgacac ctacaaggaa acggcgtttt acaagtatta
     1381 ccgcctatgg tgcaagcggg taaacccctc gatgcatatt gaacacatag ccggcgataa
     1441 gctctatgtg gattatgccg gtggaacatt gccgtatgtc gacacggata cgggcgaagt
     1501 aaaagaagca caggtgtttg ttgccatact gggatggagt caatttgcct ttgtggaagc
     1561 gatgcagaac caaaccgttc ctgaatttat tgccggatgc gaacatgcca tgcagtattg
     1621 cggtggtgtt ccgctggcat tggtgccgga taacctcaag tcagcggtat ttaaagccca
     1681 taaatacgaa ccgatcttaa atgaaaattt tagatccttt gccgcgcatt atggatgtac
     1741 gatactaccc gcaagggcaa gaaagccaca ggacaaggcc catgtagaaa acatggtaaa
     1801 gatcgtgtat caaaatattt acacggtcat gccgcaaggg gaactgctct cactctcgca
     1861 attaaatcag gccatccgta aacaactgtc cctgctcaat gcgcaactgc tcaccgataa
     1921 aaactgctca cgcagtgatc tgttggctat ggagcaaagt gcattgcatc cgcttccgga
     1981 taccatttat gagatcagaa aaataaagca ggtaacggtg atgaaaaact atcatgtgtt
     2041 gctcactgaa gatcatcact attacagtgt gccgtatgac ctgatcggta aaaagctgtc
     2101 gatgcaatac acgcgcagtg tggttgagct gtatttgaat tatgagctgg tagccaccca
     2161 tcagcgtgtg cgctctccgc ataactacac tactgagccg gcccacatgc ccgctacaca
     2221 tcaatttgta cacgaatgga gcccggggtt ctttctggaa aaagcacgtg ctattgatcc
     2281 ggtggtggag gaatatattg gacatgtact ggcgaaaaaa cagcaccccg aacaggccta
     2341 taaatcctgt cagggtatac tatccttcgc taaacgcgtt ggtcatcagc gactgatcaa
     2401 tgcctgcaag cgtggtctgg atattggata ctacaactac cgtttgatag atgatatcct
     2461 gaaaaagaac atggatgcct atgaagatga aaaacctgca gagcacatgc ccagccatga
     2521 aaacatccgt ggcggcaatt attatcaata ataaatcaat ctcattttat taatcaacta
     2581 aaaccaaaaa caatgacgga aacagtagca aaaatgaaac agatgcgcct gctgggaatg
     2641 gcacgtgcat ttacccacac gatggaatca ggcaagaatg aaagctttac gcctgatgaa
     2701 atgatcacac atctcattga ctcggaatgg gatgaacggt ataaccgaaa actggaccgg
     2761 acagtgaaag aagcccggtt ccgctataag gcaagcatcg aacaaatagg ctttgaggat
     2821 aatcgggtgg ataaaaatca gatactacgt ttagccgatt gcagcttcat taagcgtgcc
     2881 gaaaatatca tcattaccgg tagtacaggc atcggtaaaa gctttttggc atcagccata
     2941 ggtcaccagg cttgctcatt gggatacaaa gtcttgtatc agcacagcac taaactcttt
     3001 gcgcgcctta agatcgctaa agccgacggt tcttttttaa aggaactgag taaaatagaa
     3061 aagcaacatc tattactcat tgatgacttt ggcatacaac ccttagatgc acaaagcaga
     3121 tctacgctca tggagatcat cgaagatcgc catggcaaat catccgtaat catcacctcc
     3181 caagtaccgg tcaaccaatg gcacgacatc attggcgaac aaacaatcgc ggatgccatc
     3241 cttgatcgca ttgttcatga cgcccaccgc atcaatatga aaggagaatc attacgcaaa
     3301 aagcgggacc ttcaaaatga agaataaacc cttatataaa atcaaattaa ttaacttagc
     3361 tatcgaatgc taaaaacagt ccttgatcat caaaaaacaa cagtcaaaag tga
//
LOCUS       DAOQHY010000268         3410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_17805_length_3410_cov_5.265574, whole genome shotgun sequence.
ACCESSION   DAOQHY010000268 DAOQHY010000000
VERSION     DAOQHY010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17805_length_3410_cov_5.265574"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            281..1099
                     /locus_tag="PLL28_14340"
     CDS             281..1099
                     /locus_tag="PLL28_14340"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNF70557.1"
                     /translation="MEKIQITYAHPNSAGRLQHNLGIDAYRIVLLKGARGKLLVDCEY
                     YPLNPFTIAFVYPGQQFELPLQKPAEAWVISFEESFFYTNTEHKELLFRSPFFGYVLS
                     SPLFRINAKQYEMFASITSQMLHEQKLHAERSDQVMLSFLNIFLLYIKRMSWGKNTKV
                     HQNDYTTLQIVSRFKHLIRTHCHQQHSVKWYADQLQQTSNYLNLMVKKVTGGTAGDLI
                     ADQLTMEAKRLLMHTKLSPKEVAFTLGFYDHSYFSKFFKKQTGYNPLEWFNRNK"
     gene            complement(1124..2308)
                     /locus_tag="PLL28_14345"
     CDS             complement(1124..2308)
                     /locus_tag="PLL28_14345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70558.1"
                     /translation="MRNLEHYALLSGAFLAIGSNADAQIVYTDIDPDLYTDGSFQSLD
                     IDVDGIADFGFYMYFYTSWFDVQYSMVYMQALHSGAAIENAAYAWWIPSTGAIALDAG
                     AAIGSAGNFDMPPGEDLALLAALKSYNPGFSCSSSSFSPHDQLFFGFPGVGDKYVGIR
                     LGEPGNYHYGWIRLSVPYHIVCTVPMGQSFELIAHDYALNLTANASILAGEVAVDYCH
                     PPTPFTPFVGATAAKISWESVAEAEAYLVQYRQTGVATWTEKIISSTKTSKLIKSLNC
                     NTAYEWQVSSICDSGAVQSPPCALQTFTTEACRIGVDEDVLTGITLFPNPVIDALHIS
                     AEEGIHSVIILNMNGKLMKEQIFGSPEDISIDVADFPAGVYLIQLQTESGTSSDKFVK
                     AE"
     gene            complement(2418..>3410)
                     /locus_tag="PLL28_14350"
     CDS             complement(2418..>3410)
                     /locus_tag="PLL28_14350"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70559.1"
                     /translation="EREKALDRLREIQKQDFKDILMTMAPRPVTIRLLDPPIHEFLPT
                     EDVLRDELRHLRHLRETASGVSNLFGSLRLLNVDPAMANTATQPFTDKGLELIDQAIA
                     KKEQMLRKVRELYEVNPMLGHRGVRLGLTYPEIYTMQIGAILEAAAECQKVGIDVRPE
                     IMVPQVCAAKELAYVNKYVRQLKESIENQYNIQLHYKFGTMIEVVRACMEADSLAGEA
                     EFFSFGTNDLTQATFSFSREDAENKFLPWYINSGVLKDNPFEILDEHGVGKLMRFAVQ
                     EGRNTKPDLKIGICGEHGGQPEAIAFCHGLGLNYVSCSGPRVPVARLAAAQAAM"
BASE COUNT          960 a          857 c          664 g          929 t
ORIGIN      
        1 gaaacctaac aggtctaaga cctgttgatg ctctttttga aagactcact cccaacctcc
       61 ccaaagcccg ccaaaccgct tatacccgat tactcgcgat ctcctccaaa agagcctccg
      121 catatcaaaa atgagccctc atacccaatt tttgttatcg tgaaaaaata ctactaaatc
      181 gtgaaaaaat accggcgata aacgatatat ttgtaaatga aagcatgata ccattgcccg
      241 cctagttttt taagccccct tgtttttaca agtatcatgt atggagaaaa ttcaaatcac
      301 ttacgctcat cctaattccg ccggccgttt acagcataac ctcggcatag atgcttatcg
      361 cattgtccta ctcaaaggcg cacgagggaa attactcgtc gactgtgaat attatccact
      421 taacccattt accatcgctt ttgtgtaccc aggacaacaa tttgaattgc cgctgcaaaa
      481 gccggctgaa gcatgggtaa tctcctttga agagtctttc ttctatacta atactgaaca
      541 taaagaacta ctctttcgct ctccattttt tggttacgtc ctttcttcac ctctgtttag
      601 aatcaatgcc aaacaatatg aaatgttcgc ctctatcacc tcacaaatgc tgcatgagca
      661 aaaattgcat gccgaaagaa gtgatcaggt gatgctgagt ttcctcaata tatttttgct
      721 gtatatcaaa cgcatgagct ggggaaaaaa tactaaggtc catcaaaatg attataccac
      781 ccttcaaata gtttcacgct ttaaacactt aatccgcact cattgccatc aacaacatag
      841 cgtgaaatgg tacgccgatc aactccaaca aacctcaaac tatctaaacc tcatggtgaa
      901 aaaagttaca ggtggaacgg caggagatct tattgctgat caattaacca tggaagcgaa
      961 gcgcctactc atgcacacga agctctctcc aaaagaagtt gcttttacac tcggctttta
     1021 cgaccattcc tatttttcta aattcttcaa aaaacaaaca ggatacaatc cgctcgagtg
     1081 gttcaatcga aataaatgac accggtaata ttgccattcg cctttattca gcttttacaa
     1141 acttgtcaga acttgttcct gattcggttt gcaattgtat caaatacacg cctgcaggaa
     1201 aatctgcaac atctatcgaa atatcttctg gactaccgaa aatctgctct ttcatcagct
     1261 ttccgttcat atttaatata atcacggaat gtatcccttc ctcggcggaa atatgtaaag
     1321 cgtcaatcac cggattcgga aacaaagtaa ttccggttaa tacatcttcg tcgacgccta
     1381 tcctgcatgc ctctgttgta aatgtttgca gtgcacatgg tggcgattga actgctccgc
     1441 tatcgcaaat tgaacttacc tgccattcgt aagcggtatt gcaatttagc gattttatga
     1501 gcttggatgt tttggttgat gaaatgattt tctcagtcca agtagcaact cctgtctgcc
     1561 gatattgaac taagtaagcc tccgcttccg caactgattc ccaggaaatc ttggcagctg
     1621 ttgcgcctac aaacggcgta aatggagtag ggggatgaca ataatccacc gcaacttcgc
     1681 cggctagtat cgatgcattt gctgttaggt tcaaagcata atcatgggca attaattcaa
     1741 acgattgtcc cataggaact gtgcacacaa tatggtatgg caccgataaa cgaatccaac
     1801 cataatggta attaccgggc tcgcctaagc ggatacctac atatttatcg cctactccgg
     1861 gaaagccgaa aaacaactga tcgtgcggtg aaaatgacga tgagctgcac gaaaacccgg
     1921 gattataaga ctttaaagca gccagtaatg ccaaatcttc accgggaggc atatcaaaat
     1981 tgcctgcgga accaatcgct gcccctgcat ccaaagcaat ggctcccgtt gatggtatcc
     2041 accaggcata agccgcattt tcaatcgcag caccactatg taaagcctgc atgtatacca
     2101 tcgagtattg cacgtcaaac caactcgtat agaaatacat gtaaaaccca aaatcggcaa
     2161 tgccatctac atcaatatcc agcgattgga aactaccgtc ggtgtaaaga tccggatcaa
     2221 tatcggtgta tactatctgt gcatcggcat tagagccaat tgcaagaaaa gcacccgata
     2281 acagtgcata gtgttcgagg ttgcgcataa tgtgatagct taatcacatg aagctacact
     2341 atacaactcc caaacggaat gatttctgtc aacatcctaa aaccgcaccc cgcaccttta
     2401 gttgttctcc ctaaacatca catcgccgcc tgcgccgctg ccaatcttgc aaccggtacc
     2461 cttggacctg aacagctcac atagttcaat cccaatccgt gacagaaggc aatcgcctca
     2521 ggttggcctc catgctctcc gcaaataccg attttcaaat ccggtttggt gtttctacct
     2581 tcctgaaccg caaatcgcat cagcttgcct acaccatgtt catccaggat ctcaaatggg
     2641 ttgtctttca acactcctga attgatatac caaggcagga atttgttctc ggcatcctcg
     2701 cgactgaagc tgaaagttgc ctgtgtgagg tcgttggtac caaagctgaa aaactcggct
     2761 tcaccggcca aactatccgc ttccatacac gctctcacca cctcaatcat ggtaccaaat
     2821 ttgtaatgta attgtatatt atactgattt tcaatacttt ctttaagttg tctaacatat
     2881 ttgtttacat acgctagctc cttcgcagca catacttgag gtaccataat ctcaggtctt
     2941 acgtcgatcc caaccttttg gcattcggcg gcggcctcca gaatagctcc gatctgcatg
     3001 gtgtagatct cagggtaagt gagtccgaga cgcacacctc tgtgaccaag cattgggttc
     3061 acttcataca gttcgcgcac cttgcgaagc atctgctctt ttttggcaat ggcctggtcg
     3121 ataagttcga gtcctttgtc ggtgaatggc tgtgtagcag tgtttgccat ggccgggtca
     3181 acattcagca gtcgcaggct tccgaagagg ttgctgactc cggaagcggt ctcccggagg
     3241 tgacggaggt gtctgagctc atcgcgcagc acatcttctg ttgggaggaa ttcatgaata
     3301 ggtggatcga gtagacggat ggtgacagga cgaggagcca tggtcatgag tatatccttg
     3361 aagtcctgtt tctgtatctc cctcaatcta tccagcgctt tttcacgttc
//
LOCUS       DAOQHY010000269         3409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_17812_length_3409_cov_5.762970, whole genome shotgun sequence.
ACCESSION   DAOQHY010000269 DAOQHY010000000
VERSION     DAOQHY010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17812_length_3409_cov_5.762970"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1123)
                     /locus_tag="PLL28_14355"
     CDS             complement(98..1123)
                     /locus_tag="PLL28_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNF70560.1"
                     /translation="MSEIAQQLTAKDFQTDQEVRWCPGCGDYSILKQVHTVLPQLGIP
                     RENIVVVSGIGCSSRFPYYTNTFGMHSIHGRATAIASGLKAARPELSVWIVTGDGDGL
                     SIGGNHMIHLLRRNFNVNVLLFNNEIYGLTKGQYSPTSPVGKVAKSTPMGSIDHPFNP
                     AALALGADATFVARSMDRDPGHLQEILIRANQHKGTSFTEIYQNCNIFNDGAFEVFTE
                     KATKKENDLFVKNGQPLLFGEGDTKGIKLDGFTPIVVDLNDSHSKDDLWIHNENDIYK
                     AQILVRIFDDPHRDGHMPRPFGVFYENPTRATYEDMMTDQLNAAIAKGPGNLDKLLRG
                     NETWEIK"
     gene            complement(1214..3043)
                     /locus_tag="PLL28_14360"
     CDS             complement(1214..3043)
                     /locus_tag="PLL28_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNF70561.1"
                     /translation="MNIQTIEDVVIKFAGDSGDGMQLTGGQFTTNTALLGADLATFPD
                     FPAEIRAPIGTLPGVSGFQLHFGSRKIFTPGDLCDVLVAMNVAALKVNIRNLKKGAIV
                     IVNTDGFDQKNLRLANADTNPLEDGTLDGYVVIKIDVTKLTREALSEIQMGVKEKDRC
                     KNMFVLGFLYYMYSRTLDSTERFLNAKFSKKPEVLEANVKALRAGYNYADTTEVFSSR
                     YQVEAAKLEPGTYRSIMGNEAAAMGFVAASQKCGLPLFLGSYPITPASDILHYLSYNK
                     NFGVRTFQAEDEIAAICSSIGASFGGHLALTTTSGPGVALKAEAMGLAVMLEIPLVIV
                     NIQRAGPSTGMPTKTEQADLLQALYGRNGECPMPVIAAARPSDAFDVAFESCRIALNH
                     MLPVMMLSDGYIANGAEPWKFPQSSDLPEIPVKFAEPITDGSVYKPYERDEKLSRPWA
                     IPGTKGLEHRIGGLEKANITGNISYDAENHEYMVKLREEKVERIADYIPLQRIESGPE
                     KGKVLVVGWGSTYGAIQSAVNQLLADGYSVAHAHIRYLKPFPKNLGEILFNYDKVIVP
                     EINNGQLVKVLRDKYMIPAIPFNKIKGTPITSSELIDVIMKNY"
BASE COUNT          958 a          779 c          734 g          938 t
ORIGIN      
        1 atacttgata cctgatacct gatacctgat actaaatact gccagtgggt tgaaggttgt
       61 ataaactcac ccactgaccc actcacccac ttaccactca cttaatctcc caagtctcgt
      121 tgcctctgag gagcttgtcg agattgcccg ggcctttagc tatagcggca ttgagctgat
      181 cggtcatcat gtcttcgtag gttgcacgag tcgggttttc gtagaataca ccgaaaggac
      241 gcggcatatg accatcacga tgcggatcat caaaaatgcg aaccagaatc tgtgctttat
      301 agatatcatt ttcattgtgg atccaaagat catcttttga atgactatca ttcagatcaa
      361 caacaattgg tgtaaatcca tcgagtttaa tacccttggt atcaccttcg ccaaacaata
      421 atggttgacc attttttaca aagaggtcat tttctttttt agttgctttt tcagtaaata
      481 cttcaaatgc accatcattg aaaatattac agttttgata tatctcggtg aaggaagtac
      541 ccttatgttg attggcacga attaaaattt cctgcaaatg tcccggatcg cgatccatac
      601 ttcttgcaac aaaagtcgca tcggcaccca atgcaagagc agcgggatta aacggatgat
      661 cgatagaacc cataggtgtt gatttagcaa cttttcctac gggtgaagtt ggagaatatt
      721 gtcctttcgt aagaccatag atctcgttgt tgaacaataa tacattcaca ttaaaattac
      781 gacgaagtaa atgtatcatg tgattaccac caatagataa tccatcgcca tcaccggtaa
      841 cgatccacac acttaattca gggcgcgcag ctttcaatcc ggaggcaatt gcggtagcgc
      901 gtccgtgaat actatgcata ccaaaagtgt tggtatagta agggaaacgg ctgctgcaac
      961 cgataccgga aacaaccaca atattttcgc gtggaatccc aagttgtggt aatacagtat
     1021 gtacttgttt caggatagaa taatcaccac accccggaca ccaacgcact tcctggtcgg
     1081 tttgaaaatc tttcgctgtt agttgttgtg ctatttcgct cattgtaatt atatcttatt
     1141 taatttgcgc ttttcaattt ctgcatgctt gcatttttgc atgcttcaat ggtttgtata
     1201 cgcttaaatg attttaataa tttttcatga tcacatcgat cagttctgaa ctggtgatcg
     1261 gcgtgccttt aattttatta aatggaattg ccggtatcat atatttatcg cgcagcactt
     1321 tcaccagttg tccgttattg atctcaggaa caattacttt atcgtaattg aaaagtattt
     1381 caccaagatt tttagggaaa ggtttcaggt agcgaatatg tgcatgagca acagaataac
     1441 catcggcaag caattgattt accgcgctct gaatagcacc gtaagttgat ccccatccaa
     1501 caaccaaaac ctttcctttt tcaggaccgc tttcgattct ttggagcggg atataatcgg
     1561 cgatacgttc tactttttct tcgcgcaatt tcaccatgta ttcatggttt tcggcatcgt
     1621 aagagatatt tcctgtgata ttcgccttct ccaaaccacc aatacggtgt tcgagacctt
     1681 tagttcccgg aatagcccat ggacgactta atttctcatc gcgctcatat ggcttataca
     1741 ctgaaccatc ggtaataggc tcggcaaatt ttaccggaat ttcaggcaga tctgacgatt
     1801 gtgggaattt ccatggctcg gcaccgtttg caatataacc gtcgctcaac atcattaccg
     1861 gaagcatgtg gttcaatgca atacggcaag attcgaatgc cacatcaaat gcatcactag
     1921 ggcgagcagc agcaattacc ggcatagggc attcgccgtt gcgaccgtat aatgcctgca
     1981 gaagatcggc ctgttctgtt tttgtaggca tacctgtcga cggacctgca cgctgaatgt
     2041 ttacgattac cagcggaatc tccaacataa cggcaagacc catcgcttca gctttcagtg
     2101 ccacaccggg acctgaagta gttgtgagcg ccaggtgacc tccgaatgaa gctcctattg
     2161 atgaacagat ggcagcaatt tcatcttccg cctggaaggt gcgcacacca aagtttttat
     2221 tgtatgaaag gtaatgtagg atatccgatg caggtgtaat aggataagaa ccgaggaata
     2281 atggcagtcc gcatttttgc gaagcggcta caaatcccat ggcagcggcc tcattaccca
     2341 taattgagcg gtaagttccc ggctcgagtt tagcggcttc cacctgataa cgacttgaga
     2401 acacctcagt ggtatcggca tagttataac cggctctgag ggcttttacg ttagcttcca
     2461 atacttccgg cttcttgctg aacttggcat tcaggaagcg ttctgtagag tctagtgtgc
     2521 ggctatacat gtagtagagg aacccgagca cgaacatgtt cttgcaacgg tctttctctt
     2581 tcacacccat ttgaatctcg ctcagtgcct ctcttgtcag ttttgtaaca tcgattttga
     2641 tcacgacgta accatcaagc gtaccatctt caagcgggtt ggtatcggca tttgccaaac
     2701 gaaggttctt ttgatcgaac ccatcggtat ttacgataac gatagcacct tttttgaggt
     2761 tgcggatatt cactttcaat gccgccacgt tcatagctac cagcacatca cacaagtccc
     2821 ccggtgtaaa gatcttacgg ctgccaaagt gcaactggaa gccggaaaca cccggcaaag
     2881 tgcctatagg tgcacggatc tcagcgggaa agtcgggaaa ggtagccaaa tcggcaccta
     2941 aaagggcggt attggtagta aactgaccac ctgtgagctg cattccatca ccggagtcac
     3001 cggcaaattt gatcacaaca tcttcaatcg tttggatatt cataataacc gaaatctaaa
     3061 tttcggcaaa gataaagaaa gcccaaatac aaaggggtga tttttaagag aggagaaagg
     3121 agaaaggaaa gaggagagag gaaaaaggag agaggaaaaa ggagaagggg tggtttttcg
     3181 cgcgaagtgg caaagggtgt gaaccgagtt cgcagagggc aagaaatgca gaccattcac
     3241 gattcacgag tgacgagtca cgattcacga ttaaactcac ccactgaccc actcaccctt
     3301 ttcctacttt tgcattccac cagatgccta ccaaacccaa cattctagta ctatgcgggc
     3361 gcaataagca gcgcagcaaa actgctgaag aaattttcaa gcaggatca
//
LOCUS       DAOQHY010000270         3380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_18037_length_3380_cov_3.373534, whole genome shotgun sequence.
ACCESSION   DAOQHY010000270 DAOQHY010000000
VERSION     DAOQHY010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18037_length_3380_cov_3.373534"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2992)
                     /locus_tag="PLL28_14365"
     CDS             complement(<1..2992)
                     /locus_tag="PLL28_14365"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNF70562.1"
                     /translation="MLSRFLLLLLLLGAVSRGYLFSQIIINEGSNKSYLTGIDEDGEG
                     SDWIELYNNSDVPADLSGYFLSDKVSEPTLWSLTDFTLEPHAFTTIYCSEKDRHHTTP
                     FTLAIDETDFIPEVGWNTHYFTTAYDWDGVSNLIFNICSYNNTGYTENSVFQQTATPY
                     ASTSATFVDGSDASCSNVLGTLYFQRPNLQINGITIDFGSILNSNVDYPAPYGNWYWS
                     ARHQILVRAEELIAAGLSAGPISSLAFQVASTNVITYTYIDIAINSTSEDALSESMIP
                     VNGYADHTNFKIDADGENIYLNDATGSLVSSLMVNSPIADISVGRSPDGTEDIVWMTP
                     SPGMTNNAANVFTDTLISPVLSVPTSIVDDAFNLIITNPNTGAVATRVVYTLDGSEPT
                     FTSTIYTGTPIPINENIVVRARVFPETDATYLPSYDAIATYLFGVNHSTPILLVTTDN
                     INLYGATGIFDNYNSDWIKAAHAAYLSKEIGHPILFETRTAMRMDGGAGGSRANPQHS
                     FRLSFDHAALGEKTIHEQLIPAIPFRNDYSDVYLRNGSNQWLTLPYKDACQVSLMSNG
                     TNNYYSAMEPVSVYINGSYFGLYELREKFNTEYFDEREDINEDSTEILSLSYFYNLLL
                     RALEGDVDNFYADYNAFNALNPMDTDYIDQADIYFDLEHYTDYIIGESWMGNTDWPYN
                     NIKIYRSDKTNWRWRFALIDLELSLMPNSWTNCSYNHIAYMLSQGTDNPYINIWLQSI
                     QNETYLNQFINRFADLMNTAYLIDTLLAGEQAIYDKWAPEMPNEYARWGDPLNVEGQM
                     ADFTARHETFRQQLECRSDKVRGDLLSEFDLTKEVTVHLDVYPELAGTIHLNTIEPGV
                     YPWTGIYFDGVPITLTAQADSGYIFSYWEPSAFIIDTLNAHFTSNISDAEVTFTAVFK
                     QPEEPDGTTIQFNVYPSPSNGSITIAHNNATLAKDATYSIYNETGQFVMNGNLSESAL
                     TTTLNVTHLAPAIYYVRINRGGDIL"
BASE COUNT          967 a          626 c          727 g         1060 t
ORIGIN      
        1 gaagaatatc tcctccgcga ttaatgcgga cataataaat ggcgggagct aaatgcgtga
       61 cgttaagtgt ggtagttaaa gccgattctg acaaattgcc gttcatcaca aattgacctg
      121 tttcgttata tatcgaatag gtggcatctt tcgctagcgt ggcattgttg tgtgctatag
      181 taattgatcc gttgcttggt gacggatata cattgaattg tatggtagtg ccatcgggtt
      241 cttccggctg cttaaatact gcagtaaatg ttacttcggc atcactgatg tttgatgtaa
      301 agtgtgcatt cagggtatcg attataaagg cacttggctc ccaatatgaa aatatatatc
      361 cggaatcagc ttgggcagta agtgttattg gcacaccatc aaagtatatt cctgtccacg
      421 gatagactcc cggttctatg gtgttcagat ggatagtgcc tgccagttca ggataaacat
      481 caagatgcac ggtaacttcc tttgtaaggt cgaattcact taacaggtcg ccccttactt
      541 tgtcacttct gcattcgagt tgctgacgga atgtttcatg cctggcggtg aaatcggcca
      601 tttgaccttc tacattgagt gggtctcccc aacgcgcata ttcattcggc atttcaggtg
      661 cccatttgtc ataaattgcc tgttctcctg caagcagtgt atcaattaaa taagcagtat
      721 tcattaaatc ggcaaagcga ttgataaatt gattgagata tgtttcattt tgaatgcttt
      781 gtaaccagat attgatatat ggattatcgg ttccttggct aagcatgtat gcaatgtgat
      841 tatatgaaca gttggtccaa ctgtttggca ttagtgaaag ttcaagatca atgagtgcga
      901 aacgccaacg ccaatttgtt ttatcggaac gatatatttt aatgttgttg taaggccagt
      961 cggtatttcc catccatgat tcgccgataa tataatccgt ataatgttca agatcgaaat
     1021 agatatcggc ttgatcgata taatcagtat ccataggatt taatgcgttg aaggcattgt
     1081 aatcggcata gaaattgtcg acatcgcctt ctaatgcacg tagcaataaa ttatagaaat
     1141 agctcagtga aagaatttca gtcgaatctt catttatgtc ctcgcgctca tcgaaatatt
     1201 cggtgttgaa tttttcccgc aattcatata gtccgaaata ggaaccgttg atatacactg
     1261 aaacaggctc catcgcagaa taataattat tggtgccgtt gctcattaag ctcacctggc
     1321 aagcatcttt atagggtaat gtcagccatt ggttactgcc attgcgtaaa tacacatcac
     1381 tgtaatcatt gcgaaatggt atagcgggaa ttaattgctc atggatggtt ttctcaccaa
     1441 gtgctgcatg atcgaaactt aagcgaaaag agtgttgagg atttgctctg ctaccgccgg
     1501 cacctccatc catgcgcata gctgtgcggg tttcaaataa aattgggtga ccaatttcct
     1561 ttgaaaggta tgcggcatgt gcggctttta tccagtctga gttgtaattg tcgaatatac
     1621 cggttgcgcc atatagatta atattatcag tagtaaccag caatataggt gtgctgtgat
     1681 ttacgccgaa taaatatgtg gctatggcat cgtaactagg taaataagtt gcgtctgttt
     1741 ccggaaatac cctggccctt acaacaatat tttcattgat cggaattggt gtgccggtgt
     1801 aaatggttga tgtaaatgtt ggttcacttc catccaatgt gtaaacaacc cttgttgcca
     1861 ctgcacctgt attcggatta gtgatgatca aattaaaggc atcatctaca atgctcgtcg
     1921 gaactgacaa caccggagat ataagcgtgt ctgtaaaaac atttgcggca ttatttgtca
     1981 tgccgggtga tggtgtcatc caaacaatat cttcggttcc atccggactt cgaccaaccg
     2041 aaatatcagc aataggtgaa tttaccatca gtgaacttac caagcttcct gttgcatcat
     2101 taagataaat attttcgcca tccgcatcaa tcttgaaatt agtgtgatca gcatagccgt
     2161 tgacaggaat catactttct gaaagtgcat cttctgaggt gctgttgatg gcaatatcta
     2221 tatacgtata tgtgattacg tttgttgatg ccacttgaaa tgcgagtgaa gaaattggac
     2281 ctgcgcttaa tcctgcggct atcagttctt ccgcacgcac taatatctga tgacgcgcac
     2341 tccagtacca atttccataa ggagcaggat aatcaacatt tgaatttagt atggagccga
     2401 aatcaattgt gatgccgtta atttgcagat ttggtctttg aaaataaagt gttccaagta
     2461 cattgctgca cgatgcatca ctgccatcaa caaatgttgc agatgttgaa gcatatggtg
     2521 tggccgtttg ttgaaatacc gaattttcag tgtagccggt attattatat gagcaaatat
     2581 taaaaatgag attactaaca ccatcccaat catacgctgt tgtaaaataa tgtgtattcc
     2641 agccgacttc aggtataaaa tcagtttcat caattgctaa tgtaaatggt gttgtatgat
     2701 gacgatcttt ctcactgcaa tagattgttg taaatgcatg tggttcaagg gtaaaatcgg
     2761 tcaatgacca tagtgttggt tcggaaactt tatcacttaa aaagtatcca ctcaggtcgg
     2821 caggaacatc tgagttatta tacagctcta tccagtcgga gccctcacca tcttcatcta
     2881 tacccgtcag gtagcttttg ttcgagcctt cgttgatgat gatttgtgaa aataaatagc
     2941 ctctagatac agcacctaaa aggagtaata acaataggaa tcgcgacagc attgaggagt
     3001 agggttaaac tttttcgggt ttgggtttgg atatattcaa agttaaggat aatccaaata
     3061 tgtcctgata aaccgattgt aaactgatta ttttgaccta tttatgaaat atgctgctcg
     3121 gtatgttgca tgaaacgaca tgttgtagaa gatttgattc aacaaaagca ttcgaggatg
     3181 catttaacgc atatttgttg acaatcaaca aagtgattta ccctgatgcg ttctcataaa
     3241 atacttacaa gctaatgttt agtgtcagtg aaattgatgg tgtatagata ttgtggatgg
     3301 tagtaagact aggctctgca acattttcaa tggcagggta gtcgcttgat ataggtatgt
     3361 atgttcgggt tgattgaaac
//
LOCUS       DAOQHY010000271         3367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_18159_length_3367_cov_2.672403, whole genome shotgun sequence.
ACCESSION   DAOQHY010000271 DAOQHY010000000
VERSION     DAOQHY010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18159_length_3367_cov_2.672403"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3367
                     /locus_tag="PLL28_14370"
     CDS             <1..>3367
                     /locus_tag="PLL28_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70563.1"
                     /translation="EEAINLHASNYIAQLEADNPECFSTIDELLAATEDFEITCEISC
                     DYCQTLGSLDDFLSAEFPNPEDLDNPKPEIGSPEYAFYENIYNDLMDMCAQVCPENVM
                     SPCAAGVNAMLADLAPGGQYAEYKIDADDNYSANDYALSILNLDNNLLDRSIVTTPVD
                     EPEAAAYKYPITYFDGQWHNGYYKDGVRETVDLSIYFALDPLLTGIYDGPLTLSDLDG
                     DGVSESAIGYPEYLTLKYFIQFYQYDWGYGLLLYHPEYYYYAACLNYSACTSEIVPPV
                     EAPMLNLSTFTFDEQMLAYTYDDIMTSVPWKKPYTIDPLFDNTTCTVDGVDYETFVTN
                     YIEDYGYCEMGFAPDVHLNIYQVLYLTMNYPELDFCDAMPSEIDDYADIDDMATLGVI
                     NSTESWLYFTILYKAAKTKYLNALSHAYAQNYYSINLSIQNDAYWPPVITTLNDLAVA
                     DPWDEVTTNTAYLPYYTRSVELSTKSVRFPDMSWHNGLPPNLAYDDPMLDDLADEVTA
                     DVDYAYYTETGNLPMSEDFMVMLNLVTSQAEDAAIDWRTTPVETDDIIGISARLRDAL
                     DEYCLGTWDAAMSTVNHANDKLTWSIATGCTPTGACSDNYIQLDHLATPAVNYGWDEE
                     LVGFSDIITELDGNFTIIAVFSIDGEYVHLPAHGHSCIPFDQTSYLQTHCDLTSDAAG
                     VWSVMNYLIGINQLNATSYNLDPTLVPEILTTYLLPIDPTSHYIWRHVADGEYSLSNI
                     DNAHTIRLSMTTGDDLSGANFISNFEPDPELIPAGYPIVTTPIDLFTLSQNGGITFKS
                     GYISYTNTAMAGGSYVLHSFPVATCGHDAMLEQPCQEPEHFSAFNLNDLLAELLGNAD
                     PIIEDTYIALESPDIYPGWTTHLSTMMGGSDVQDEFMYIQSYTGNTLDIIFKTEIATD
                     TYVDLCHVSLTIADPLAPLSLEDLSANILLVPHAELAEADGIHDFTLECTDGSCTDDE
                     IATIAWGYIDCLTLKACPCPDKNINGSFYYDVVTDVTIEGGVIGVVGDHPITGVHDAE
                     SYSIGTTEKTIMLDYQTYLKSGKIDITETAELKLQLFAFGKEALIDTIYADSTADTIT
                     VYLRLDGYDAVGSNLRTGIGECSSNPIETPYIPY"
BASE COUNT          981 a          696 c          669 g         1021 t
ORIGIN      
        1 gaagaagcca taaacttaca tgcttcaaat tatatcgctc aactggaggc tgacaatccg
       61 gaatgtttca gcacaattga tgaactgctt gcagctacag aagattttga aattacttgt
      121 gagatcagtt gtgattattg tcaaacattg ggttcattag acgattttct ctctgctgaa
      181 tttccgaacc ccgaggattt agataatcca aaaccggaaa taggaagccc ggaatatgca
      241 ttttatgaaa atatctacaa tgatttaatg gacatgtgcg ctcaggtatg ccccgaaaac
      301 gtgatgagcc cttgtgcagc aggtgtgaat gccatgttag ccgaccttgc ccccgggggt
      361 caatatgctg aatataagat agatgcagat gataattatt cggcaaacga ctacgcactt
      421 tcaattttaa atcttgataa taatcttctt gatcgctcaa ttgttacaac acccgttgac
      481 gaacctgaag ctgctgcata taaatatccg attacttatt ttgacggtca atggcataat
      541 ggctattata aagacggtgt aagagaaaca gttgatcttt ctatatattt tgcgcttgac
      601 ccgttattga caggaatata tgatggacca cttacattga gcgatttaga tggagatggg
      661 gtatctgaaa gtgctatcgg ttatcctgaa tatttaacct tgaaatactt cattcaattc
      721 tatcagtatg actggggtta tggtttatta ttgtatcatc ctgaatatta ttattacgca
      781 gcatgcctta attattctgc ctgcacctca gaaattgtac caccggttga agcacctatg
      841 ctaaacctct caacattcac atttgatgag caaatgctgg cttataccta tgatgatatc
      901 atgacttctg tgccatggaa aaaaccttat acaatcgacc ccctttttga caacacaact
      961 tgcactgttg atggtgtcga ctatgaaaca tttgtgacaa attatatcga ggattacggt
     1021 tattgtgaaa tgggttttgc gcctgatgtt catttaaaca tatatcaggt gctctattta
     1081 acaatgaatt accctgaact cgacttctgt gatgcaatgc cttcagagat tgacgattat
     1141 gcagacatag atgatatggc aaccttagga gtgatcaatt caaccgagtc atggttgtat
     1201 tttaccatcc tatataaagc agcaaaaaca aaatatctga atgcactttc acatgcatac
     1261 gcacaaaact attacagcat caatttaagc atacagaatg atgcttattg gcctccggta
     1321 attaccacgc taaatgattt ggctgttgct gatccttggg atgaggtaac aactaatact
     1381 gcctatttac catattacac cagatccgta gagctttcaa caaaaagtgt tcgattcccg
     1441 gatatgtcat ggcataacgg gctcccacct aatcttgctt atgacgatcc gatgttggat
     1501 gacttggctg atgaagttac tgctgatgtc gactacgcat attatactga aacgggcaat
     1561 ctgcctatgt cagaggattt tatggttatg ctaaaccttg ttacctcaca ggcagaagat
     1621 gcagcaatcg actggagaac tacacctgta gaaactgatg atatcatcgg tatttcagcg
     1681 cgactccgcg atgcactgga tgaatattgt cttggaacat gggacgctgc catgtcaact
     1741 gtcaaccatg ccaatgacaa actcacatgg agcattgcta ccggttgcac cccaaccgga
     1801 gcctgcagcg ataattatat tcagttggac catttggcaa cacctgcagt aaattatggt
     1861 tgggatgagg aacttgttgg attttctgat atcataaccg aacttgatgg gaattttaca
     1921 atcattgctg ttttctctat tgatggcgaa tatgttcatt tgcctgccca cggccattca
     1981 tgtatcccgt ttgatcaaac atcttatttg caaacacact gcgatcttac ttctgatgca
     2041 gccggtgtat ggagcgtaat gaattatttg attggtataa atcaattgaa tgcaacttca
     2101 tacaatttgg atccaacttt ggttcctgaa atattaacca cttacctatt acctattgat
     2161 cccacatcac attatatctg gcggcatgtt gctgatggtg aatattcact ttcaaatatc
     2221 gataatgctc ataccataag attgtcaatg actaccggcg atgacctgag tggtgcaaat
     2281 ttcatcagca attttgagcc agatcctgaa ttaattcctg ccggctatcc aattgtaaca
     2341 acaccaatcg atttgttcac gctatcgcaa aatgggggca taactttcaa atcaggttat
     2401 attagttata cgaatacagc tatggcaggt ggaagctatg tactccattc atttcctgta
     2461 gccacttgcg gccatgacgc catgcttgag caaccatgcc aggaaccgga gcatttttca
     2521 gcctttaatt taaatgattt gttagccgaa ttgcttggca atgctgatcc gatcatcgaa
     2581 gatacttata tcgctttaga atctcctgac atttatccgg gctggaccac tcaccttagc
     2641 acaatgatgg gtggatcgga tgttcaagat gaattcatgt atattcaaag ctatacagga
     2701 aacacattgg atatcatctt taaaactgaa attgcaacag atacttatgt agacctttgc
     2761 catgtgtcgc ttactattgc tgacccactg gcgccgctat ctctggagga tttgagtgct
     2821 aatattttat tggtgcctca tgccgaatta gctgaagccg atggcattca tgatttcacc
     2881 ttggaatgca cagatggctc atgcaccgat gacgaaattg caactattgc gtggggctac
     2941 atagattgtt taacactcaa agcatgtcct tgtcctgaca aaaacattaa tggctcattc
     3001 tactatgatg ttgtaactga tgtcacaatt gaaggcggtg ttataggtgt tgttggcgat
     3061 cacccgatca ccggtgtgca tgatgccgaa tcatattcaa ttggcaccac agaaaaaacg
     3121 attatgcttg attatcaaac atatttgaaa tcaggcaaga tcgatatcac tgagactgct
     3181 gaactgaaat tgcaactttt tgcttttggg aaggaggctt tgatcgacac tatttatgcc
     3241 gattcaactg ccgacaccat aactgtttat ctccgtcttg atggttacga tgcagtaggt
     3301 tcaaatctac gtaccggcat aggcgaatgt tcatcaaacc ccatagaaac accatatata
     3361 ccatata
//
LOCUS       DAOQHY010000272         3353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_18281_length_3353_cov_4.576410, whole genome shotgun sequence.
ACCESSION   DAOQHY010000272 DAOQHY010000000
VERSION     DAOQHY010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18281_length_3353_cov_4.576410"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1378)
                     /locus_tag="PLL28_14375"
     CDS             complement(<1..1378)
                     /locus_tag="PLL28_14375"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNF70564.1"
                     /translation="MNYLQAEGLSKTYDTKVLFDNISFTINRGQKVALVARNGAGKTT
                     LLRVLTGKDFADAGKVSVHKSITLGILEQDPMLDESLSIIDNVLSGDSPSIRAIRAYE
                     EALMAQEHHFNEETSTALERAHDAMHALNAWDYELRIKQILSHLNITELDQKVSTLSG
                     GQKKRIALSKLLIEEPDMMILDEPTNHLDIEMVEWLEEYLTSRNITLLLVTHDRYFLD
                     RVCNEIIELENGKVFSYRGNYSLYVEKKAHRDMVEAAELEKVRNTYRRELEWVRRMPK
                     ARTTKSKSRLDAFDEIAEKAKGKQKQEALTLDVKMTRMGGKILEMIKVSKAFGTLKIV
                     EQFTYLFKQYDKIGIIGKNGMGKTTFLNLIMELEKPDAGKITTGETVVYGYYSQSGMV
                     LKEDKRVIEVVKDIAEFIPLADGSKLSAAQLLTKFQFPPETQYTFVSKLSGGEKRRLY
                     LMTILIKNP"
     gene            1996..2661
                     /locus_tag="PLL28_14380"
     CDS             1996..2661
                     /locus_tag="PLL28_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70565.1"
                     /translation="MVKTIPTKRGTGISIYGLAHDLKYVYDVVHYIATTLDEGDVQLK
                     GRHQLLMNFAYEIRKAYQEARLKKTFIQQNGEEVEYLGFNIVWTDILIFLNVLRDQAT
                     YLPTNKRQQSVLYSLESSVEEAFRAYDLESADGILSFIEHRFFVRSQYCFILYQAVHI
                     EFVGQAGGKRRFKKLPLLLSSYFSEFHEVHIDIINRLESTAKQQNCDPTELGYSEFPE
                     IRW"
     gene            complement(3024..>3353)
                     /locus_tag="PLL28_14385"
     CDS             complement(3024..>3353)
                     /locus_tag="PLL28_14385"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70566.1"
                     /translation="KEESYPEVIDEIPQIEYRQIDEVIKQSNADFRIYPNPADELVFI
                     ELTGKTDCENFLYVYDITGVRVLATALRNDYKLTWIDTEGFQTGIYFFEVFSNGNTFG
                     AEKVIIE"
BASE COUNT          901 a          734 c          668 g         1050 t
ORIGIN      
        1 ttggattttt gatgaggatg gtcatcaggt ataacctgcg tttttcgcca ccactcaatt
       61 tactcacaaa cgtatattgc gtttcgggcg gaaactgaaa cttggtaagt aattgcgctg
      121 cactgagctt agatccatcg gcgagtggaa tgaattcggc gatgtctttc actacttcga
      181 tcacgcgttt gtcttccttc aacaccattc ccgactgcga gtaataacca tacaccacgg
      241 tctcgccggt agtgatcttt cctgcatccg gtttttcgag ttccatgatc aggttgagga
      301 atgtggtttt tcccattcca ttcttcccaa tgatgccgat cttgtcgtat tgcttgaata
      361 aataggtgaa ctgctctaca attttcagag tgccaaatgc ttttgacact ttgatcatct
      421 caaggatctt gccgcccatg cgtgtcattt ttacatcgag ggtgagggct tcctgctttt
      481 gttttccttt tgctttttcg gcgatctcat cgaacgcatc caggcgcgat ttgcttttgg
      541 tggtgcgggc tttgggcatg cggcgcaccc attccaactc gcgacgatag gtattgcgca
      601 ccttctctaa ttcagctgct tccaccatat cgcgatgcgc tttcttttct acatagagcg
      661 aataatttcc acgataacta aaaacttttc cattctctaa ttcaatgatc tcattgcaca
      721 cgcgatcgag aaaatatcta tcgtgcgtta ctaacaataa agtgatattc ctcgatgtca
      781 gatactcttc cagccactcc accatttcaa tatcaaggtg gttggtaggc tcatcaagta
      841 tcatcatatc cggttcttct atcagtaatt tgctcagcgc gatacgtttt ttttgtccac
      901 cactcaaagt gctcaccttc tgatcaagct cggtgatatt gaggtgactg agtatctgtt
      961 tgatacgcaa ttcataatcc caggcattaa gcgcatgcat cgcatcgtgt gcgcgttcga
     1021 gagcggttga tgtttcttcg ttgaaatgat gttcctgagc catcaaggct tcttcgtatg
     1081 cccggattgc gcggatggaa ggggagtcgc cactcagcac attatcgatg atactgagcg
     1141 actcatcgag catcggatcc tgctctaaaa tacccagggt gatactctta tgcacagaaa
     1201 ctttaccggc gtcggcaaag tcttttcctg tcaacactct caacaacgtg gtctttccgg
     1261 cgccatttct cgccaccagc gccactttct gacccctatt gatcgtaaac gaaatattat
     1321 caaataacac cttggtgtcg tatgtcttgg ataaaccctc tgcctgcaaa taattcacgc
     1381 ggcaaaggta gggaatgtgg ggggataggg tggggaatta gcaattgaag gattccaaaa
     1441 ttctattgca ataattgaaa gctaaattgg agctcggctt catttcttcg aagaaatccc
     1501 gccaagccta tacaggagtt gagacaacac ctgtttagca cccatgtgtt taggagtttg
     1561 tcatgatttt acctcagtca atcaccctca cacgcaacct accttatgca aagtgcatcc
     1621 tcacttattt cagatcgctc aatccgcccg gttagcatct tgctgtgttt tcactccgtt
     1681 cactcggaat agctaactta agtctaccgg ctgctggcat tttaggctcg ccttcgatgt
     1741 agaatgggct acgaggttga catggttttt gagtcaaaaa cacacgccaa ccccttgtgt
     1801 agtccgaagg cttatgcctt cttctacata ggtcgcaacc gccttgcttg ttggcaagga
     1861 ctttcttgct ctcacccaat ctccgctcgc agctcccgtt atttcgcttt gctccattcc
     1921 gtctgcttaa cggcgagcag tatatcggga atgctttcta taatttcaat aattttgatg
     1981 tgaaatattt aatagatggt caagacaatt ccaacaaaaa gaggaacagg tatatcgata
     2041 tatgggctag cgcatgatct caaatatgta tatgatgtcg ttcactacat cgcaacaact
     2101 cttgatgaag gagacgtaca actgaagggc aggcatcaac tcctgatgaa tttcgcatat
     2161 gaaattcgga aggcatacca agaagcacgt cttaaaaaga cttttataca acaaaatggt
     2221 gaagaagttg aataccttgg gtttaatatc gtatggactg atattttgat attcttgaat
     2281 gtcctgcgtg accaagcaac ttatttgcca acaaataaac gacagcaatc tgttttatac
     2341 tcacttgaat ccagtgttga agaagcgttt cgagcatatg atcttgaaag tgctgatggc
     2401 atactgtcat tcattgaaca ccgtttcttt gtccgcagtc agtattgctt catcctttac
     2461 caggctgttc atatcgagtt tgttgggcag gcaggtggaa aaagacgatt caagaaatta
     2521 ccattacttc tcagtagcta tttttctgaa tttcacgaag tccatattga tataattaat
     2581 agactggaaa gcacggcgaa acaacagaat tgcgatccga ctgagcttgg atattcggag
     2641 tttccggaga ttaggtggta atcaagggtg tcctacacct aaatctttta ataaatcata
     2701 tcatgtagga caaaacagaa ttatgccatt caaatggttt atttatttcc tgcagagctt
     2761 atttgacaac cctctttagt atttacagag ggattgccaa ataagtatta atatgatggt
     2821 caccaaaagt aactgtagga cgatcgaagt ataagtggaa ttaccttatt aaagaaccgt
     2881 atacatatta gtagtttaca agaaatgaat attgatttcc aaatgcagag ataataaata
     2941 gtacaaaatg gtctgtttcg ctctttttat taagacacgc atttggtgtg ttttcagtat
     3001 ttgttttgta actgatttta ctgttactca ataattactt tctctgcacc aaatgtattg
     3061 ccattgctaa aaacttcaaa aaagtatata ccggtttgaa agccctctgt atctatccat
     3121 gtcagcttgt aatcatttct taaagctgta gcaaggactc ttacaccggt gatatcataa
     3181 acatagagaa agttctcgca atctgtttta ccggtgagct caataaatac aagttcatct
     3241 gctgggtttg gatatatcct gaaatctgca tttgattgtt taattacttc atcaatctgt
     3301 ctatactcaa tttgaggtat ttcatcaata acctcaggat acgattcctc ttt
//
LOCUS       DAOQHY010000273         3336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_18438_length_3336_cov_5.652850, whole genome shotgun sequence.
ACCESSION   DAOQHY010000273 DAOQHY010000000
VERSION     DAOQHY010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18438_length_3336_cov_5.652850"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="PLL28_14390"
     CDS             complement(<1..1110)
                     /locus_tag="PLL28_14390"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HNF70567.1"
                     /translation="MQKRITQLLCGVLLTTTPLLSNAQLTLPPSGDNQQSAVSQWMGP
                     VRVDITYSSPDVHAPDGSDRTGHIWGELVPYGMVNLQFGYSNENHPSPWRGGANMNTT
                     ISFSHDVLVNGSALKAGTYGLHFIPNKDEWTVIFSSNSTSWGSFFYKPEEDVLRVQTK
                     PAANNYHEWLTYEFTDRQTDACTVELMWELLRVPISIKVPDIKQVYVENFRKQLRSDV
                     GFNYQAWVDAANYCVANNVNLQEALTWANYAIEEPFFGRVNFATLSCKAGVLKALGKT
                     VQSDSIMKVAIADPTASLMDVHFYARGLQAEKKNKEALEIFLINYQKHPESPVTNLGL
                     ARGYSANGDYKNALKYAKAAQKLNPDPQVKQTLENA"
     gene            1206..1967
                     /locus_tag="PLL28_14395"
     CDS             1206..1967
                     /locus_tag="PLL28_14395"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNF70568.1"
                     /translation="MTEKQLIAYRKEVENCIHKIEPSKSSFKGFDLQHYIGTAFTVSG
                     VKMPVARKLSRDGFSFSHLEADEQYIIWKYIWHTTDNFDTMTQAIFFCDKYIRLANPE
                     FFFNEMCEWLQRVDNWAHSDSLTHYFAALMESHPAMVYPVLQKWNKSDFSWERRQSVL
                     SLLDYARFRKKYPTFNKIIALVKPLLKDKDVFVQKGVGWCLRETGIIYPTETYAFLVD
                     NHAAISSIAFSAAAEKLSPAKKEKLKSLRKKSRKS"
     gene            1964..2590
                     /locus_tag="PLL28_14400"
     CDS             1964..2590
                     /locus_tag="PLL28_14400"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNF70569.1"
                     /translation="MMDVLSLQQKLPLLEEALIREIIERAQEVTIPPQQQILREGQYI
                     KTIPVVMNGLLKVITGSGDKDMLLYYIKPGDSCIMSFAFSLSNEPSNIAAFTETETTM
                     LLLAVDDVRQWLIAYPSLNMLFLNLYNHRYRDLIDTIGLIYEKMDNRIMHYLEQKVKI
                     TGNNIVNITHREIAADLASSREVISRLLKKLELEGKLQQTPAGIKILV"
     gene            2754..2957
                     /locus_tag="PLL28_14405"
     CDS             2754..2957
                     /locus_tag="PLL28_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNF70570.1"
                     /translation="MKKNMGSTDRIIRVILAIVFALLYFTNTVTGTLGLVLVILGGVF
                     LLTALVRFCPLYAPFGISTCKTK"
BASE COUNT          997 a          765 c          607 g          967 t
ORIGIN      
        1 cgcattttcc aaggtttgct ttacctgtgg atcaggattt aatttctgcg ccgcttttgc
       61 atatttcaat gcattcttat aatcaccatt cgctgaatat ccccgtgcca atcccaaatt
      121 tgtcaccggc gattcaggat gcttttgata attaattaaa aatatctcca gcgcttcctt
      181 attctttttc tctgcttgca atcctcttgc ataaaaatgc acatccatta aagacgccgt
      241 aggatccgca atggccactt tcataatgct gtcactctga acagttttgc ccaaggcctt
      301 caacacacct gctttacaac ttaaggttgc gaaattcact cttccgaaaa atggctcttc
      361 tattgcataa ttcgcccatg tcaatgcctc ctgtaaattc acattattag caacacaata
      421 atttgccgca tctacccacg cttgataatt aaatcctaca tcagaccgca attgcttcct
      481 gaaattttct acatacactt gtttgatatc aggcacctta atggaaatcg gcacacgcaa
      541 taattcccac atcaattcaa cggtgcatgc atcagtctgc ctgtcggtaa attcataggt
      601 aagccactcg tgataattat ttgcggccgg cttcgtctgc acacgcaaca catcttcttc
      661 aggcttatag aaaaaacttc cccacgatgt tgaattcgac gaaaatatta ccgtccattc
      721 atctttattc ggaataaaat gcaatccata agttccggct tttaaggcac tgccgtttac
      781 caagacatca tgcgaaaaag aaatggtagt attcatattc gcaccaccac gccatggaga
      841 aggatgattt tcattggaat atccaaactg caaattcacc atgccatagg gcaccagttc
      901 tccccaaata tgtccggtcc ggtcactgcc atccggcgca tgcacatcgg gcgaggaata
      961 ggtgatgtcg acacgcacag gccccatcca ttgacttacc gccgattgct gattatcacc
     1021 actgggcgga agtgtgagct gtgcgtttga caataaaggt gtggttgtca ataacacccc
     1081 acagagtaat tgagtaattc gcttttgcat agttgatcgt tttcaggata aatttatttc
     1141 tattatcatt gagggaggtt aagatttgtt aatattatgc tgctacagaa ataaattaga
     1201 atgctatgac cgaaaaacaa ctgatagcct atagaaaaga agttgaaaat tgcatccata
     1261 agatagaacc atccaaatct tctttcaaag gattcgacct gcaacattat attggcactg
     1321 catttactgt cagtggtgtc aaaatgcctg ttgccagaaa attatcccgc gatggattta
     1381 gttttagtca cctggaagca gacgaacaat acatcatctg gaaatatatc tggcatacaa
     1441 cagataattt cgataccatg acacaggcaa tatttttttg tgataaatat attcgcctcg
     1501 ccaacccgga attcttcttc aacgaaatgt gcgaatggtt gcagcgtgtc gacaattggg
     1561 cgcatagcga tagccttacg cattattttg ccgcactgat ggaaagtcat cctgcaatgg
     1621 tatatcccgt tctgcaaaaa tggaataagt ctgattttag ctgggagcga cgacaatctg
     1681 tcctcagcct ccttgattat gcacgtttca gaaaaaaata tccgactttc aataaaatca
     1741 tcgcattggt gaaacctctc ttaaaagaca aagatgtgtt cgtgcaaaaa ggcgtcggat
     1801 ggtgtctgag agaaacagga attatttatc cgactgaaac ttatgcattt ctggtcgata
     1861 accatgcagc catcagtagc attgccttta gcgccgctgc tgaaaaattg tcacctgcaa
     1921 aaaaagagaa attaaaatcc cttcgaaaaa aatcccgaaa atcatgatgg atgtattaag
     1981 cctgcaacaa aaactaccct tattggaaga agcacttatc cgggaaatca ttgagcgcgc
     2041 tcaagaggtc accattcctc cgcaacaaca aattttgcgg gaaggtcaat atatcaaaac
     2101 aatacctgtt gtgatgaatg gcttgctaaa agtaattacc ggtagcggcg ataaggatat
     2161 gttgttgtat tacatcaagc ccggcgatag ttgtatcatg tcatttgcct tcagtctgtc
     2221 aaatgaacca agtaatattg ccgcattcac agaaacagaa actaccatgc tcttgcttgc
     2281 cgttgatgat gtccgacaat ggcttatcgc ctacccttca ctcaacatgt tatttctcaa
     2341 tctgtataat catcgatatc gcgatttaat agataccatc ggacttatct acgagaaaat
     2401 ggataaccgc atcatgcact acctcgaaca aaaagtgaag atcacgggaa ataacatagt
     2461 caatatcaca catcgcgaaa ttgcagccga cctagcaagc agcagagaag taatttcccg
     2521 ccttttgaaa aaacttgaat tggaagggaa actgcaacaa acacccgcag gaataaaaat
     2581 ccttgtttga tgtgaccaca gtcacaagcc atggccccgg cttcggtcta cctttgactt
     2641 atcaatgcaa cagaaacgat atatgaaatg ggtgctcatg tgcctcagct gtatcggaat
     2701 agcaaccctg atcgcattgg tcattcaact catcaaacaa taaactacgt attatgaaaa
     2761 agaacatggg ctcaaccgac cgcattattc gcgttattct cgcaatcgtt ttcgctttac
     2821 tttattttac aaacaccgtt accggcacac ttggacttgt tttagtaatt cttggtggcg
     2881 tgttcctctt aaccgcattg gtgcgctttt gccccctgta tgcacctttc ggtatcagca
     2941 cttgtaaaac caagtaatta atagttcaaa atataacata agtccgtcaa aatgcttggc
     3001 ggaattatat ttttataatt aacaccggca ttcaaaatct aatagttttg tattcgctat
     3061 cgtactccac gctccccccg cctttagatt attccggtaa agcacaagcg ggaattgttt
     3121 ctgtaatgac atatttttct gcattaacat ctccaacact tgcaggctgc ggtttttctc
     3181 tatctttgaa ttctacatta aacttttaat tagaatgatt aaacaagtta ctcctctttt
     3241 aatgggtatg ctttgcatat gtggcgtggc caatgcccaa ttgaacacgg aattattagg
     3301 tcatctccct tatgccgatc agctttcaaa cctcac
//
LOCUS       DAOQHY010000274         3318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_18581_length_3318_cov_4.714067, whole genome shotgun sequence.
ACCESSION   DAOQHY010000274 DAOQHY010000000
VERSION     DAOQHY010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18581_length_3318_cov_4.714067"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1494
                     /locus_tag="PLL28_14410"
     CDS             <1..1494
                     /locus_tag="PLL28_14410"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HNF70571.1"
                     /translation="SYFSIDDCTPGSEIRSYFSASYGAWNYGDDWLLCDNDNSWTNTG
                     SYLMVMTSLWSTRKAMNYYLNVHGHESFDGSGGLIDLFSNKTYFDDDDLPYCRNANYT
                     NIIDNLNFGSGNDCMPGTMDDYNTLDIVGHEFTHGVIEYAHFDALDYSDESGALNESF
                     ADIFGEMVEYYTEGGDTLSWLCGEDRGAIRSFKNPKAFGDPDTYYGTNWATLGGTDNG
                     GVHTNSSVQNHMFYLLSQGGSGVNDHGIEYQVEGIGYDHARDIAWVAMMDYLDGSDGY
                     IIARNAWIQAAIDLFGSCSQEVISVGQAFQAAGVTEYTAFDRASVCGTYVLSGYADAT
                     YGIENSTMVLGDFLIDCTTTINATAAVTFKSGYYIQLNPGFTALSGCNLTAYISACEI
                     SEYDPDNVRIAQSNLQSPTVAEMDDKLLVFPNPVFDIVTINWEHEIPMEQLQIVNIAG
                     QNVTDHISIEEQSLGKTIIDMRNLSAGMYMIVIKTADGEAYGNCIKQ"
     gene            complement(1565..>3318)
                     /locus_tag="PLL28_14415"
     CDS             complement(1565..>3318)
                     /locus_tag="PLL28_14415"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNF70572.1"
                     /translation="PIFCRKKGTLDAKEEVLVDGNELAKGHNFLSYEVEVSPDETKAC
                     IIMDVHGRNFYTIRVRDLQTKQWLSDEVPGTRNGCVWSRDNNSFVYGVPDTVTLRVYQ
                     VKRHFLGMKASDDKLLFEEKDPTLDIYVYGSRSEDFIFAYSGRTDANTVWFADARDPR
                     QFKQVAPLVENVVFNVDHVKGEEFTILTNYKAKNYRLCSVNYFDPRMDNWQDVIPGRD
                     DVFLTQVQYFSNFMVAEEVQNGLTMLRVIVNDKTDVPIKFDEPAYYATLGYNPDYHAT
                     GFRYHYSSLITPFTTYHYDVSSSLTEILKQQEIPSGYKKDLYKTERIMVTARDGKQVP
                     LTISYRTDMFHKNGTNPGFIYGYGSYGYSNEDYFDANILSLLDRGFVYANTHIRGGQE
                     MGGEWYDDGKMMNKKNTFYDFIDCSIWLQKNNYVASDKLFANGGSAGGLLMGAILNMA
                     PTTYKGVIADVPFVDVITTMEDESIPLTTFEWLEWGNPKIKEQYDYMMSYSPYDNVTA
                     QNYPNILVTTGLNDSQVQYFEPTKWVAKLRYTKTDKNMLLLKVNMDAGHGGRSGRFES
                     VNEVAFMYAFMLQCLGI"
BASE COUNT          935 a          665 c          696 g         1022 t
ORIGIN      
        1 agctattttt ctatcgatga ttgcacgccg ggttcggaga taaggagcta tttcagtgca
       61 tcgtatggtg catggaatta tggcgatgat tggctgttat gtgataatga taattcttgg
      121 acaaatacag gttcttacct gatggtgatg acttcattgt ggagtactag aaaggcaatg
      181 aattattatt taaatgtgca tggtcacgaa agctttgatg gatcgggagg cctgatagat
      241 ctttttagta ataaaacata tttcgatgat gatgatctcc cttattgccg aaatgctaat
      301 tacaccaata ttatcgacaa tctgaatttt ggttcgggaa atgattgtat gcccggaacc
      361 atggatgact ataatacatt ggatattgtt ggccatgagt tcacacacgg tgtcatcgaa
      421 tatgcccact tcgatgcatt agattactcc gatgaaagcg gtgctttgaa tgaatcattt
      481 gccgacattt tcggagagat ggtggagtat tataccgaag gcggtgatac ccttagttgg
      541 ctttgtggtg aagatagggg tgccatcaga tcttttaaaa atccaaaagc ttttggcgac
      601 cctgatacat attatggcac taattgggca acattaggag gcactgataa tggcggagtg
      661 cataccaata gcagtgtgca aaatcatatg ttttatttgc tcagtcaggg tggttcaggt
      721 gttaatgacc atggaattga gtatcaggta gagggcatcg gttatgatca tgcaagagat
      781 attgcctggg ttgcgatgat ggattatctc gatggttctg atggatacat cattgcacgt
      841 aatgcatgga tacaagctgc aatcgatttg tttggttcat gttctcaaga ggtcatctca
      901 gttggacaag catttcaggc agcgggagtt acagagtata cggcctttga tagggcatcc
      961 gtttgcggaa cctatgtgct gagtggttat gcagatgcaa cttatggcat tgaaaattca
     1021 accatggtgc ttggcgattt cctaattgat tgcactacaa ctattaatgc aacagccgca
     1081 gttacattca agtcgggata ttatattcag ttaaatcccg gatttacggc attgagcgga
     1141 tgtaatctca cagcatatat cagtgcatgc gaaatcagtg agtacgatcc tgataatgtg
     1201 cgtattgcgc aatcaaactt gcagtctccc acagttgccg aaatggatga taaattactg
     1261 gtgtttccaa atccggtttt tgacatcgta acgatcaact gggagcatga aattccgatg
     1321 gagcaactgc aaatcgtgaa tattgcaggt caaaatgtta cggatcatat ctctattgaa
     1381 gagcaatcat tgggtaagac aattattgat atgcgaaacc tgtccgcagg tatgtatatg
     1441 attgtcatca aaacagccga tggcgaggca tatggaaatt gtatcaaaca ataaatgttg
     1501 gctgtaagta cacaaaaaag ccgacaaata atcgtcggct tttttgttat ttcatttggc
     1561 ataattaaat ccccaaacac tgcaacataa atgcatacat aaaggctact tcattaacgc
     1621 tttcgaacct tccggatcga ccgccgtgac ctgcatccat gtttactttt aatagcaaca
     1681 tgtttttgtc tgttttggta tagcgcaatt ttgccaccca ctttgtcggc tcaaaatatt
     1741 gtacctgcga atcgttcaat cctgtggtga ctaatatgtt cggataattc tgagcagtta
     1801 cattatcata tggagagtaa ctcatcatat aatcatattg ctccttgatt tttgggtttc
     1861 cccattccag ccattcaaat gtagtaagtg gaatgctttc gtcttccatg gtggtgatca
     1921 catcaacaaa tggaacatca gcaattacgc ctttataggt ggttggtgcc atgtttaata
     1981 ttgcacccat taataatcca ccggcactac cgccattggc aaacaattta tctgaggcca
     2041 cataattatt tttttgcaac cagatactgc aatcgatgaa atcgtagaaa gtattctttt
     2101 tattcatcat cttgccatca tcataccatt cgccacccat ttcctgacca cctcgaatat
     2161 gtgtattcgc atacacaaat cctctatcca acaaactcaa tatgttggcg tcaaaataat
     2221 cttcattgct atatccatat gagccgtaac cataaatgaa tccgggattg gtgccatttt
     2281 tatgaaacat atctgtacga tagctaatag tgagtggcac ctgcttgcca tcgcgggcgg
     2341 taaccataat gcgctcagtt ttgtataaat cttttttata gccactcgga atttcctgct
     2401 gtttcaatat ttctgtcagc gatgaagaaa catcatagtg ataagtggta aatggtgtga
     2461 tgagcgagga ataatgatat ctgaatccgg tagcatgata atccggattg tagccaagcg
     2521 ttgcataata tgcaggctcg tcgaatttaa tcggcacatc tgttttatca ttcacaatta
     2581 cacgtagcat tgtcaatccg ttttgcactt cttctgctac catgaaattg ctgaagtatt
     2641 gcacttgagt taaaaataca tcatctcttc cgggtataac atcctgccaa ttatccatgc
     2701 ggggatcgaa ataattcacg ctacaaaggc gatagttttt agccttgtag tttgtgagaa
     2761 tagtaaattc ttcaccttta acatgatcga cattgaatac aacgttttct accaatggcg
     2821 caacttgttt aaattgacga ggatcacgtg cgtcggcaaa ccaaacggta tttgcatctg
     2881 tgcgtcctga ataggcgaaa ataaaatctt cactgcggct gccgtaaaca tagatgtcga
     2941 gcgtaggatc tttttcttcg aaaagcaatt tgtcatctga agctttcatt cccaggaaat
     3001 gccgctttac ctgatacaca cgcagggtta ctgtatccgg aacaccatat acgaatgagt
     3061 tattatctcg gctccataca catccatttc ttgtgccggg cacctcgtcg cttaaccatt
     3121 gctttgtttg cagatctcga acgcgaatgg tgtaaaaatt gcgaccatgc acatccataa
     3181 taatgcatgc tttggtttca tcgggactga cttcaacttc gtagctcagg aaattatgcc
     3241 ctttcgccag ttcgtttcca tcaaccagca cttcctcctt tgcatccaga gttcccttct
     3301 tacggcagaa gatcggat
//
LOCUS       DAOQHY010000275         3317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_18590_length_3317_cov_6.978541, whole genome shotgun sequence.
ACCESSION   DAOQHY010000275 DAOQHY010000000
VERSION     DAOQHY010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18590_length_3317_cov_6.978541"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(616..2064)
                     /locus_tag="PLL28_14420"
     CDS             complement(616..2064)
                     /locus_tag="PLL28_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70573.1"
                     /translation="MAQPKLITIYGALSERERERFGEFVRSPFHNKRSKLINLVAHLE
                     VMFAGEEPKIWDKHEAYHCVFEADTPGKKRPAFDELQLNNVVSDLFRLMEDFLAWQQF
                     SRQPEWRDVMMVEHLLSSSMLEQADKVIRKKASNEMSDAYAQYRFNQLADQHFFQRSR
                     KGDNRYLIQGQQSLEIYFLANQLRIWCELINRANVLSLAYDKQQFADFEQYLLLSLPK
                     YGNQPTISIYWPILTWLRDQENDTWFIGFKEKLFQHIQEFPEQEGKDIIAYVQNYCVK
                     RINEGKNAFLREWYEIAQYMLPLRLLHEGQWISQWTYKNIVTAGVRLKEFAWTEQFAH
                     DYYTALSQEERDNAYQYNLAVIYYEKHDIDRAMQLLNRVHFSDPNYYLDAKTILLKIY
                     YEHNAFEALISLRDTVKIYLLREKLLSRSQQMLYKNLFSLTLKLYKLRLEKESTDPEK
                     WRKQFELLKKGISENNLVANKGWLMGKMEEVN"
     gene            2234..>3317
                     /locus_tag="PLL28_14425"
     CDS             2234..>3317
                     /locus_tag="PLL28_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70574.1"
                     /translation="MKRLLFLLFAISALSLTAQVPWSYAHQGTDTWPAANAKDLLVGA
                     DDETFVFGDIGLIDINEGAFTLAAIASDGSVVWEQTYDKPGTLEMGMRMSRTSDDGFL
                     LSGMTAPDYRPYFVRTDADGNEMWNSASVTDLLPENSASQCYAYELPSGNIAYVFSDD
                     VYKFLTRIEVDPATGAAISTTTLNYLDILPAGIGTVITANTVDMITNNSGDLIVIADF
                     MATGADTTDYIFGINSDFELAGRFGISNGAPNYYANSVIQNADGDYIITGTKNTGAFF
                     DLYSPMITLVQGGFLWSSTLSFTPTLSQATGGAITQTTEGGYKWIRYQQNGTYFDPAV
                     GDYVEVATLDMWLTETATHTINYAPYN"
BASE COUNT          927 a          705 c          670 g         1015 t
ORIGIN      
        1 ccgcaaatct agctaaacat tgcaggcaaa tgcaaactca atggtgagag gtgagtggtg
       61 agtggtgaat ggtgagtggt gagtgggtga gtggtggctc gtgaattttt acctgatact
      121 tgatacttga tactaaattc cgaatactat cacaatcctc aaaaacccat ttaaccacag
      181 agaccacgga gatgaacaca gaggtgcaca gagtaaattc ccggcacatt ccaccgcatt
      241 tatctcaata ctcactaccc actaccgaat tgtgagcggt aagtggtgag tttgggcgca
      301 ctttagcatc ggatatatga tgcaaatatg catttacctt acacaccaat caccattcac
      361 gactcacgat tcacccttgt tcaatcgcgg agtttgcgga gttagcggag tcagtggatc
      421 agtcaccact caccattcac gactcacgat tcacccttgt tcaatcgcgg agtttgtgga
      481 gttagcggag tcagtggatc agtcaccact aaccattcac tattcacgac tctcgagtca
      541 cgagtcacga ttcacgaaac actcacccac tcaccattgc atttccattt gctaatcagc
      601 taatttgcta attggctaat taacttcctc cattttcccc atcaaccatc ccttattcgc
      661 aactaaatta ttttcactga ttcccttttt cagcagttca aattgcttgc gccacttttc
      721 aggatcggta gattcttttt ctaaccgcag tttatataat ttgagtgtta aggaaaagag
      781 gtttttatat agcatttgct gactgcgact gagcaatttt tctcgtaata aatatatttt
      841 cactgtgtcg cgaagagata tcagcgcttc aaatgcattg tgttcgtagt aaatttttaa
      901 aagtatggtt ttggcatcca gataataatt cggatcgctg aaatgtactc ggttgagtaa
      961 ttgcatggcg cggtcgatat cgtgcttttc gtaatagata acggcaagat tatattgata
     1021 agcattatca cgctcctcct gacttaatgc agtataataa tcgtgagcga attgttctgt
     1081 ccatgcgaat tcctttagtc gcactccggc tgtaacgatg tttttataag tccattggga
     1141 gatccattgt ccttcatgca gcaacctgag cggtaacatg tattgtgcta tctcatacca
     1201 ttcgcgaaga aatgcatttt taccttcatt gatacgtttt acgcagtagt tttgaacata
     1261 ggcaatgatg tcttttcctt cctgttcagg gaattcttga atatgctgaa atagtttttc
     1321 cttaaatcct ataaaccagg tgtcattttc ctgatcgcgc agccatgtga gtatgggcca
     1381 ataaatgctg atagttggtt gattgccgta tttcggtaaa cttaaaagta agtattgttc
     1441 gaaatcagca aactgttgtt tgtcgtaggc aagtgataat acattagcgc gattaattaa
     1501 ttcacaccaa atgcgtaatt gatttgccag aaaatatatt tcaagtgatt gctggccttg
     1561 tatcaaatag cggttatcgc cttttcgcga ccgttgaaaa aaatgttgat cggccaattg
     1621 attgaaacga tattgtgcat aggcgtcact catttcattg gaggcttttt ttctgatcac
     1681 cttatcggct tgctcaagca tcgaagacga taataaatgt tccaccatca tcacatcgcg
     1741 ccattcgggt tgccgactga attgctgcca tgccagaaaa tcttccatca acctaaaaag
     1801 gtcgctcact acattattta gttgcagctc atcgaaagca ggtctttttt tgccgggggt
     1861 gtctgcctcg aaaacacagt ggtaagcttc atgtttatcc caaattttag gctcctcgcc
     1921 ggcaaacatc acctccagat gagcaacaag gtttatcagc ttactgcgct tgttatgaaa
     1981 aggactgcgc acaaattcgc caaaacgctc ccgttcccgc tcggaaagtg caccgtaaat
     2041 ggtaataagt ttcggttgcg ccatggctat aaaattggcg ttatattaaa cataaagact
     2101 tgaaaaacaa tgttttacct atatttgata tgagcatttt ggctataaaa catggttaaa
     2161 agtaattgtt tttacccgga actgtatcgt tctttgttgc aatgatttcc ataaacatta
     2221 aatttataac atcatgaagc gccttctttt tctactattc gcaatttcag cactctcgct
     2281 tactgcccag gtgccttgga gttatgccca tcaaggtact gatacctggc ctgctgccaa
     2341 tgccaaagat cttttggttg gtgcagatga cgagaccttt gtgtttggtg atatcggctt
     2401 gatcgatatc aatgaaggcg ctttcaccct cgcagctatc gcatcagacg gcagcgtggt
     2461 gtgggaacaa acttatgata aaccgggtac gcttgaaatg ggtatgcgca tgtcgcgtac
     2521 atccgatgat ggatttctgt tgagtggaat gacagcaccg gattatcgcc catattttgt
     2581 gcgcacagat gccgatggaa atgaaatgtg gaattcggca tcggtaacag atttacttcc
     2641 tgaaaattca gcatcgcaat gttatgcata tgaattgcct tcaggaaata ttgcttacgt
     2701 attttccgat gatgtatata aatttcttac gcgtattgaa gtcgaccctg caacaggtgc
     2761 cgcaatcagt accactacat tgaattatct cgacatactt cctgcaggaa ttggtactgt
     2821 catcactgcc aatacagtag atatgatcac caataacagt ggcgatctga tcgtgattgc
     2881 agattttatg gcaacaggtg ccgatacaac cgattatatt tttggtatta attccgattt
     2941 cgaacttgca ggacgatttg gtatttcaaa tggtgctccc aattattatg caaatagtgt
     3001 aattcaaaat gccgatggtg attatatcat cacaggaaca aaaaataccg gtgctttctt
     3061 tgatctttat tcaccaatga tcacacttgt gcagggtgga tttttgtgga gttcaacact
     3121 ttcgtttaca ccaacattat cacaagcaac cggtggcgcc atcacccaaa caacagaagg
     3181 tggatataaa tggatacgct atcaacaaaa cggaacttat ttcgatcctg ctgttggcga
     3241 ttatgtagaa gtggcaacat tagacatgtg gcttaccgaa acagcaacac atactatcaa
     3301 ctatgcacct tataata
//
LOCUS       DAOQHY010000276         3285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_18881_length_3285_cov_6.278019, whole genome shotgun sequence.
ACCESSION   DAOQHY010000276 DAOQHY010000000
VERSION     DAOQHY010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18881_length_3285_cov_6.278019"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(636..1043)
                     /locus_tag="PLL28_14430"
     CDS             complement(636..1043)
                     /locus_tag="PLL28_14430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HNF70575.1"
                     /translation="MQTPSKEKDYLFIKRSQIKGAGKGLYTAIDIYKDEVIAYFDGEI
                     IDDDEAFRRAEKGKDKYFVILPEGKVMDSMPVDCFAKYANDAAAYPNGEFKNNASIRL
                     DDDDKPCLIANRKIKSGEEIFCGYGRAYWKKHG"
     gene            1453..>3285
                     /locus_tag="PLL28_14435"
     CDS             1453..>3285
                     /locus_tag="PLL28_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNF70576.1"
                     /translation="MDTFSYIANAEPAYIESLYHSFRTDPSSIDGEWRKFFEGFDFAQ
                     ANFSSNGSASVSSESLTDEFRTYALILAYREKGHLVATTNPLRPRKDRHANLKTEDFG
                     FSKGDMSKKMASGSLVGMQNATLEAIIAHLEACYCNNLGFEYDYIIEPAEKKWFEQQI
                     ESRAGNFGFSAEKKRRILSKLNETVVFEQFLDKKYIGQKRFSLEGGESTIPALDAIIN
                     AGASAGVVEFVFAMAHRGRLNVLANILGKTYEQIFNEFEGNAIPDTTMGDGDVKYHLG
                     YSSQVTTLSGNTVHLKLTPNPSHLEAVNPVLQGFVRAKSDVLYNEDEDRILPICIHGD
                     AAIAGQGVVYEVLQMSKLNGYKVGGTIHFVINNQIGFTTDFDDARSSNYCTSYAATVQ
                     APVIHVNGDDAEAVVFACELAVAYRQEFNKDVFVDMVCYRKHGHNEGDDPKYTQPTLY
                     KIIDAKKNPREIYVQKLLASDAIDRELANAMEQEFWNVLQQRLDAVKQHPLTYQYQPP
                     EEAWRKLKKVIDVEKDFATSPETKVTHAQLQSVLDKLFTTPDGFVKLKKIEKLFETNK
                     KLIAEGRIDWALGELIAYGTILLDGRDVRMSGQDVKRGTFSHRHA"
BASE COUNT          961 a          705 c          725 g          894 t
ORIGIN      
        1 gaggcgaagg cgcgaagtgt actgcacccg aacacaatta gagtgtgcaa gtgtggaagt
       61 gtgcaagtgt gcaagtaacg ctgaggtggg atgtcgctgc gctcaatgtg caggtaaatg
      121 caacacccga acacaatcat tgtctgaatc acggattatt gggattaaag gattgcacgg
      181 attttcggca taggcacatt ccacaaatca acccgcacgc aaacacatga acacaatcat
      241 tgtctgaatc acggattatt gggattaaag gattgcacgg attttcggca taggcacatt
      301 ccacaaatca acccacacac aaacacatga acacaatcat tgtctgaatc acggattatt
      361 gggattaaag gattgcacgg attttcggca taggcacatt ccacaaatca acccacacac
      421 gaacacctga acacacaaac acatgaccac acgcccacaa agaaactccg gcatttaatt
      481 aaattgtatt ccctattaac gcgccagcgt taaaaggaat cacacttgct aacttgctaa
      541 ccctcacact tgcaaaccaa aacaaaaatc tccatgcaag tattaacctt aattagctaa
      601 tctgctaatt cccaaatttg ctaattacca caccttcacc catgtttctt ccaataagcc
      661 cttccatatc cgcaaaaaat ctcctcaccg gattttattt ttctgtttgc tattaggcat
      721 ggtttatcgt catcgtcgag gcgtattgat gcattatttt tgaattctcc attcggatac
      781 gctgctgcat cattggcgta tttggcaaaa caatctaccg gcatcgagtc cattactttt
      841 ccttccggaa gaatgacaaa atatttatcc tttccttttt ccgcgcgacg aaaggcttca
      901 tcgtcatcaa tgatctcacc gtcgaaatac gcgatcactt catctttgta aatatcaata
      961 gccgtgtata gccctttacc tgctcctttg atttgcgaac gtttgatgaa taaataatct
     1021 ttttcctttg aaggggtttg catttgtttg aattatcatg caaaattaat cgtgaatcgt
     1081 gattcgtgaa tggtgagtgg gtcagtgggt gaggtttggt atatgcttcg gattgggatg
     1141 cgcggcgtga atcgtgagtc gtgaatcgtg agtgtgtttc ggaggatttt atagctcgcg
     1201 ggcttcatcg ccctatggtg attcacgact cacgatttgg tagtgagtag tgggtattga
     1261 gtagtgagat caggtatcag gtatcaagta tcaggtatca agtatcaggt atcaagtatc
     1321 aggtatcaag tatcaggtat caagtatcag gtattgacaa attcactact caccactgac
     1381 ccactcacca ctcaccattc actatctttg ccctccaaac caaagcgccc gcggctttgt
     1441 taataggcat agatggacac tttctcttac attgcgaacg ccgaaccggc atatatagag
     1501 tcgctttacc atagttttcg gactgatcct agcagtatag atggcgaatg gcgcaaattt
     1561 tttgaaggat ttgatttcgc tcaagctaat ttttcttcaa atggctcagc gtcagtaagt
     1621 tctgaatcgc tgacagacga gttccgcacc tacgcgctca ttctcgctta tcgtgaaaag
     1681 gggcatctgg tggccaccac caaccccctc aggccgcgaa aagaccgcca tgccaacctc
     1741 aaaacggaag attttgggtt ttctaaaggc gatatgagca aaaaaatggc atcaggttcc
     1801 ctggtcggga tgcaaaatgc caccctggaa gccataattg cccatttaga ggcttgctat
     1861 tgcaataacc tgggctttga gtatgattat attatcgaac cggctgagaa aaagtggttc
     1921 gaacagcaga tagaatcccg tgcaggcaat tttggcttct cggcagaaaa gaaacgccgc
     1981 atcctgtcca agctgaatga aactgttgtt tttgagcagt ttctggataa aaaatacata
     2041 ggacaaaagc gattttccct cgaaggtggt gaaagcacca ttccggcgct tgacgccatt
     2101 atcaatgcag gtgcaagtgc aggcgttgtg gagttcgtat ttgccatggc acaccgcgga
     2161 agattaaatg tgttggcaaa tattcttgga aagacatacg aacagatttt caatgaattt
     2221 gaaggcaatg ccattcctga taccaccatg ggtgatggcg atgtgaaata tcacctcggt
     2281 tattcatcgc aggttactac cttgagtgga aacactgtgc atcttaagct cactccgaat
     2341 ccttcgcatc ttgaagcagt aaatcctgtt ttgcagggtt ttgtccgcgc aaaatcagat
     2401 gtattatata atgaggatga agaccgtatt cttccgatat gcattcatgg tgatgcagct
     2461 atcgccggac aaggtgttgt ttacgaggtg ttgcagatga gtaaattgaa tggatataaa
     2521 gttggtggca ccattcattt tgtgatcaac aatcagatcg gttttactac cgattttgat
     2581 gatgcgcgtt caagcaatta ttgcaccagc tatgcagcta cagtgcaggc gcctgttata
     2641 catgtgaatg gcgatgatgc agaagctgtt gtatttgcat gcgaattggc agtggcgtat
     2701 cgacaagaat tcaataaaga tgtatttgta gatatggtgt gttatcgcaa gcatggtcac
     2761 aatgaaggcg atgatcctaa atacacgcaa ccaacattat ataagatcat cgatgccaaa
     2821 aaaaatccgc gcgaaattta tgtgcagaaa ttattggcga gtgatgctat cgatcgcgaa
     2881 ttggcaaatg ccatggaaca ggaattttgg aatgtattgc aacagcgtct cgatgccgta
     2941 aagcaacatc cgcttacata tcaatatcaa ccaccggaag aagcatggcg taaactgaaa
     3001 aaggtgattg atgttgaaaa agatttcgct acatcccccg aaacaaaagt gacacacgca
     3061 cagttgcaat ctgttttaga taaattattc actacgcctg atggatttgt caaattaaag
     3121 aaaatagaaa agctgttcga gacaaataaa aaactcatcg cagaagggcg tatcgattgg
     3181 gcattgggtg aattgatcgc atacggaact atattgcttg atggtagaga tgtgcgcatg
     3241 agcggtcagg atgtcaaacg cggcacattc agtcaccgcc acgcc
//
LOCUS       DAOQHY010000277         3249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_19228_length_3249_cov_6.578898, whole genome shotgun sequence.
ACCESSION   DAOQHY010000277 DAOQHY010000000
VERSION     DAOQHY010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19228_length_3249_cov_6.578898"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..342)
                     /locus_tag="PLL28_14440"
     CDS             complement(85..342)
                     /locus_tag="PLL28_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70577.1"
                     /translation="MQEFTQWAIFMGFIALMAAILYAYASGWVNSPRTRRESFKEWQK
                     LNGGVVRMMSLLVILVCVIVCIYKYNDIKAAIDAPPTLQTE"
     gene            complement(353..1699)
                     /locus_tag="PLL28_14445"
     CDS             complement(353..1699)
                     /locus_tag="PLL28_14445"
                     /inference="COORDINATES: protein motif:HMM:NF019285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HNF70578.1"
                     /translation="MVESIFRGLLGVAFLIGVCYLFSNNRKSIDWKLVGSGLLIQFVF
                     AFVIIALPDITEQHFGYRFDWFERIFQGMANFFVMVIGFTNAGANFVLGDWPSVTQVS
                     DGLTGQVFTVGFIFAFKVLPTVIFFSALTSLLYYLGILQKIVFGFAWVMSKTMRLSGA
                     ESLNAAGNIFLGQTEAPLLIRPYLEKMTKSEVLCVMVGGMATIAGGVMAAYIGFLGGD
                     DIEKQKMFAMHLLSASIMSAPAAIVAAKMLFPQKENVDTELKVNKERIGSNVIESIAN
                     GTTDGLKLAVNVGAMLLAFTAFMAMFNAIFLGIGNMLSINDNIAAATNNTYSGLSLDY
                     IFGIIMAPVAWLLGVPSSDMLMVGQLLGEKTIINEFVAYSSLGNMKSMLDEKSIIIAT
                     YALCGFSNFASIGIQIGGIGALAPNQKTTLSQLGLRALLGGTVACFFTAAIAGMII"
     gene            complement(1726..2106)
                     /gene="crcB"
                     /locus_tag="PLL28_14450"
     CDS             complement(1726..2106)
                     /gene="crcB"
                     /locus_tag="PLL28_14450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNF70579.1"
                     /translation="MSFSTIIWIFLGGGFGTLVRYGIGTQVSGLSKSNFPIGTLAVNL
                     IGCLIIGLIYGYFEKHQETQSDWKLILTIGFCGGFTTFSAFSIDAIQLVKGGNLLLAI
                     LYIFSSVFIGILLSILGWQIAKNY"
     gene            complement(2109..2741)
                     /locus_tag="PLL28_14455"
     CDS             complement(2109..2741)
                     /locus_tag="PLL28_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF151 domain-containing protein"
                     /protein_id="HNF70580.1"
                     /translation="MQKVELEITGLSHTITQSQSYAVLLSEVHGQRKLPIVIGAFEAQ
                     AIAVAIEKVVINRPLSHDLIKNMFTVFQINLREVLIDNLQEGIFYSKLICEKDGQEFD
                     IDSRTSDALALAVRFNCPIYTYEFILDSAGAIINEPEPDDDNEEDENEEEDIDALEEL
                     SPTGFSSESSSDLTNMSLKELNERLREFLEHEDYESAARIRDEIEKRKKP"
     gene            complement(2800..>3249)
                     /locus_tag="PLL28_14460"
     CDS             complement(2800..>3249)
                     /locus_tag="PLL28_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNF70581.1"
                     /translation="DFADSDFDYVITGVEDEAGGHVPLPEAEIVVSGGRGLKGPEHWG
                     IVEDLAKALGAATACSRPVADIGWRPHHEHVGQTGITIKPTLYIAVGISGAIQHLAGV
                     NQSKVIVVINKDPEAPFFKAADYGIVGDAFEVLPRITEAVKKLHAGA"
BASE COUNT         1003 a          737 c          590 g          919 t
ORIGIN      
        1 agtaaaggaa gaatgaaaga ggctactaga ttgtatgtat ttttttcaaa tcacccatat
       61 acagcacacc tgtttaaatg atcatcattc agtttgcaaa gtaggtggtg catcaatggc
      121 tgctttgata tcattgtatt tataaataca aacgattacg caaactaaaa taaccagaag
      181 gctcatcatt ctgactactc caccatttag tttctgccat tctttaaagc tttctcttct
      241 ggtacgtgga ctatttaccc aacctgaagc ataggcgtat aatatggcag ccatcagggc
      301 gataaagccc atgaagattg cccattgtgt aaattcctgc atcgtaatat tattaaatga
      361 tcataccggc tattgcagct gtaaaaaagc atgctactgt gcctccgagt aacgcgcgga
      421 gacctaattg tgaaagtgtt gttttttggt tcggggcaag ggcaccaatt ccaccaatct
      481 gaatgccaat tgaagcaaaa ttgctaaatc cgcaaagtgc ataggttgct ataatgattg
      541 acttttcatc caacatcgat ttcatattcc ccaaactgga ataagcgacg aattcattaa
      601 taatagtttt ttcaccaagt aattgtccaa ccattagcat gtcagaactg ggtactccaa
      661 gaagccaagc cacaggtgcc ataatgatac cgaagatgta atccaaagag agccctgaat
      721 atgtattatt agtagccgca gcgatattgt cgtttattga aagcatattt cctatgccaa
      781 gaaatatggc attaaacata gccataaaag cagtgaacgc caaaagcata gcaccaacat
      841 taactgcaag ttttagtcca tcggtggtgc cattggcaat agattcaata acattactac
      901 caattctttc tttatttact ttaagttctg tatcaacatt ttccttttga ggaaacagca
      961 tttttgcagc aacaatggca gcaggtgcac tcattattga tgcgctgaga agatgcatgg
     1021 caaacatttt ctgcttttct atatcatcgc cacccaaaaa accgatatat gcagccatca
     1081 ctccacctgc aatagtagcc atgccgccaa ccataacaca taacacttca gattttgtca
     1141 ttttctcaag atatggcctg atcaacaagg gagcctcggt ttgtccaagg aatatgttac
     1201 ctgcagcgtt taaactctct gcgccgctca gacgcattgt tttactcatc acccatgcaa
     1261 atccaaaaac gattttttgt aatatgccta aataataaag caaactggtt agtgcactaa
     1321 aaaaaataac agttggtaaa actttaaacg caaaaatgaa tccaacggta aacacttgtc
     1381 cggttaatcc atcagaaact tgtgttacag atggccagtc tccgagcacg aaatttgctc
     1441 ctgcatttgt gaacccgata accatcacaa agaaattcgc cattccttga aaaattcttt
     1501 caaaccaatc aaacctgtat ccaaaatgtt gttcagtgat atcaggtagc gcaataatca
     1561 caaatgcaaa tacaaattga attagcaagc ctgaacctac cagtttccaa tctatagact
     1621 ttctattgtt gctgaatagg taacaaaccc cgattaaaaa ggctacacca agtaagccac
     1681 ggaaaatact ttctaccata aaagttaata attgtgttgc ggttttcagt aatttttagc
     1741 gatttgccaa ccgagaatag aaagcaaaat gcctataaat acactgctaa agatatataa
     1801 aatcgccaat agcagatttc cgcccttaac cagttggatg gcatctatcg aaaaagctga
     1861 aaaggttgta aagcccccgc aaaagccaat tgttaaaatg agcttccagt cgctctgagt
     1921 ttcctgatgt ttctcaaagt atccgtagat caatccaatg attagacatc cgattaagtt
     1981 aacagcaagc gttcctatcg ggaaattaga cttggaaagg ccggaaactt gtgtgcctat
     2041 accatatctc accaaggtgc caaatccacc accaagaaat atccatatta tggtggaaaa
     2101 cgacattatt aaggtttttt ccttttttct atctcgtcgc ggatgcgtgc agcagattcg
     2161 taatcttcat gctccagaaa ctccctaagg cgttcattga gttctttgag cgacatattc
     2221 gtgaggtcag aactgctttc tgatgaaaat ccggttgggg ataattcttc taatgcatcg
     2281 atatcttcct cctcattctc atcttcttcg ttatcatcat caggttcggg ctcgttgatg
     2341 atggcacctg cactgtcgag tataaactca taggtataaa tcgggcaatt aaatcgcact
     2401 gctaaagcta gcgcatcgct cgttctggag tcaatgtcga actcctgccc atccttttca
     2461 cagatcaatt tggaataaaa tatgccctct tgcagattat caattagcac ttcacgcaga
     2521 ttgatctgaa aaacggtaaa catgtttttg ataagatcgt gcgacaaagg cctgttaatt
     2581 accacttttt caatggcaac agcaattgcc tgagcctcga aagcaccgat aactatcggt
     2641 aacttgcgtt gaccatgcac ctcactcaat aacacggcat agctctgcga ctgggtaatg
     2701 gtatgtgaaa gccctgttat ttcaagttca actttctgca tgcgtacagg ctaagataca
     2761 acttactgaa tgagttaaca atgataagca attcgataat tatgccccgg catgaagttt
     2821 cttcacagcc tctgtaatgc gtggcagcac ttcgaatgca tcgccaacaa taccataatc
     2881 tgcggcttta aagaatggcg cttcaggatc tttattgatt acaacgatca ctttgctttg
     2941 gtttactccg gcaagatgct gaatcgctcc ggaaatacca actgcaatat ataatgttgg
     3001 cttgatcgtg atacctgttt gtccaacgtg ttcatgatgc ggacgccatc cgatatctgc
     3061 taccgggcgt gagcatgcag tagcagcgcc caatgccttc gcaagatctt caacaattcc
     3121 ccaatgttcc gggcctttca atccacgtcc gcctgaaacc actatctccg cttccggcag
     3181 tggcacatga ccacctgctt catcttcaac accggtgata acataatcga aatcagaatc
     3241 agcaaaatc
//
LOCUS       DAOQHY010000278         3195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_19777_length_3195_cov_7.000637, whole genome shotgun sequence.
ACCESSION   DAOQHY010000278 DAOQHY010000000
VERSION     DAOQHY010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19777_length_3195_cov_7.000637"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(313..1041)
                     /locus_tag="PLL28_14465"
     CDS             complement(313..1041)
                     /locus_tag="PLL28_14465"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HNF70582.1"
                     /translation="MPNTQEDNRPIIEVCLTPALWYQHDPKDKNVVVIDIIRATSTIT
                     SALHHGVAKMVPVQTVEEANAYLEKGFLVAGERDGYKIPHFHFGNSPFEFMGDDVKGK
                     TIIHTTTNGTRCVLMSKGAHKIVAGSFLNQEAIFNWIRKENRNTILFCAGWKDHYVMD
                     DALFAGAVVRELADDFRIIDDSTLSAKLLYRYVRENMLRTIKQATHFKRLQRLGIEQD
                     IKFCMSNPHFDVVPVFDGEGFVKG"
     gene            complement(1072..2163)
                     /gene="gcvT"
                     /locus_tag="PLL28_14470"
     CDS             complement(1072..2163)
                     /gene="gcvT"
                     /locus_tag="PLL28_14470"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HNF70583.1"
                     /translation="MKQTALTNLHKQLGAKMAEFAGYEMPINYSSQVEEHHTVRNGVG
                     VFDVSHMGEFVLKGPAAAALIQKVTSNDVYALSPGKAQYSCLPNENGGIVDDLLVYHL
                     EDGNYMLVVNASNIEKDWNHISKYNTEGVEMVNISDKTSLLAVQGPKAAEALQSLTDV
                     DLKAIPYYNFTKHRFAGVDNVIISATGYTGAGGFEIYFPAEHSLEIWNKVFEAGAAFG
                     IKPIGLAARDTLRLEMGFCLYGNDIDDTTSPLEAGLGWITKLKAANNEFVGKDKIVAG
                     KEAGLTRKLVGFEMIDRGIPRHDYPIVNAEGAVIGKVTSGTQSPSLNKAIGLGYVENA
                     YSAVDSEIFIDIRQKPVKAKVVKIPFLQQ"
     gene            2259..3194
                     /locus_tag="PLL28_14475"
     CDS             2259..3194
                     /locus_tag="PLL28_14475"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNF70584.1"
                     /translation="MQFNQAHSLTDIAALIGAKVLADPGTPVTGINEIHKVTPGDISF
                     VDHPKYYDKALNSAATFVIINKEVECPAGKGLLLSDDPFRDYVALVKHFSPFNPSLQP
                     VAKNAVVGEGTICFPGVYIGNNVIIGKNCVLHPNVVIYDNSIIGDNVIIHANTTIGSD
                     AFYFKKRATYWDKLVSCGRVIIHDDVEIGSNCTIDKGVSGDTIIGRSTKFDNHIHIGH
                     GVVIGEMCLFAAGVMIGGKTIIGNQVTLWGQVGVSKTLTIGNNVTVLAKSGVDKDLAP
                     NKIYWGAPAVESRRAWKQLAAANNMLEIWEKMKRG"
BASE COUNT          854 a          744 c          669 g          928 t
ORIGIN      
        1 acattcccta ttaacgcgcc agcgttaata gcaccaccac ttgctaactt gctaattctc
       61 gcctcacccc ccgcccgcct cacccccggc ccctctccat ctcctatcgt cgatagagag
      121 gggagataat ggcgcttcgg atttaatatt ttgcaaaaca atataattac attccctatt
      181 aacgcgccag cgttaatagc accaccactt gctaacttgc taactctcac acttgctaac
      241 taagaggata aacccgatgc aagcaatcac ccccatttgc taatctgcta attctcaaat
      301 ttgctaatta aatcaccctt tcacaaatcc ctctccatca aaaaccggca ccacatcaaa
      361 gtgcggattg ctcatgcaga atttaatgtc ttgttcaatg ccgaggcgtt gcaggcgttt
      421 gaagtgtgtt gcctgcttaa tggtgcgcag catgttttcg cgcacatagc ggtataataa
      481 tttagcactc agtgtactat catctataat gcgaaaatca tcagccagtt cgcggacaac
      541 ggcgccggca aaaagtgcat catccattac atagtgatct ttccatcctg cgcaaaacaa
      601 aatggtattg cgattttctt tacgtatcca gttgaagatg gcctcctgat tcaggaatga
      661 accggccaca attttatgtg cgcctttact catcaataca catcttgttc cattggttgt
      721 tgtatggata attgttttcc ctttcacatc atctcccata aattcgaagg gagaatttcc
      781 gaaatggaaa tgtggtattt tatatccatc gcgttcaccg gcaaccagaa atcctttttc
      841 gagataagca tttgcttctt ccacagtttg cacaggaacc attttagcaa ccccatgatg
      901 caatgccgaa gtgatggtgg atgtagctcg aatgatatcg atcaccacca cattcttatc
      961 cttcgggtcg tgttgatacc acaacgccgg ggtaaggcat acttcaatga tgggacgatt
     1021 atcttcttgt gtgttgggca taattcgggt aatagccggt taatgtcgca attattgctg
     1081 taggaaaggg attttcacta ctttggcttt cacgggcttc tggcgaatat ctataaaaat
     1141 ttctgaatca acagcactat atgcattttc tacataaccc aatccgattg ccttattcaa
     1201 gctagggctc tgggttccgc tggttacctt accaattacc gctccttccg cattcacgat
     1261 tgggtagtcg tgacggggaa taccgcgatc tatcatctca aaacccacaa gtttacgcgt
     1321 caaaccggcc tcttttccgg ctacaatctt gtccttgccc acgaattcat tgtttgcggc
     1381 tttcagcttg gtaatccagc ccaaacctgc ttccagcgga cttgttgtgt catcaatatc
     1441 gttgccatag aggcagaaac ccatttctaa acgcaaggta tcgcgagctg ctagacctat
     1501 aggtttgatg ccaaaggcgg ctcctgcttc aaatacttta ttccagattt ccagcgaatg
     1561 ttcggcaggg aaataaatct cgaaaccacc agcgccggta tagccggttg ctgaaataat
     1621 tacattatcg acacccgcaa agcgatgttt tgtgaaatta taataaggaa tcgccttcaa
     1681 atccacgtct gtcaaggatt gcagcgcttc cgccgctttc ggaccctgca ccgcgaggag
     1741 actggtttta tcggaaatgt tcaccatctc cacaccttcc gtattgtatt tgctgatatg
     1801 attccagtct ttttcaatgt tggaagcatt caccaccagc atataattgc cgtcttcgag
     1861 gtggtatacc aacaggtcgt ctacgatacc gccattttcg ttcggaaggc agctgtattg
     1921 cgcttttccg ggacttaagg catacacatc atttgaggtc accttttgta tcaaagctgc
     1981 tgccgcagga cctttcagca cgaactcacc catgtggctc acatcgaaaa caccaacgcc
     2041 gttgcgtacg gtatgatgtt cttctacttg tgaactataa ttaatgggca tctcataccc
     2101 tgcaaactcg gccattttgg cgccaagttg tttgtgaagg ttggttaagg ctgtctgctt
     2161 catgtgtttc gattaaagcg agcgcaaaga tacggtttgc taaaggattt cactgtgtga
     2221 tgtgaggcgt tatgcatttt gtttcagacc tttgcgccat gcaattcaac caagcacatt
     2281 cactgacaga tattgctgca cttataggcg cgaaagtatt ggctgatccc ggaacaccgg
     2341 ttacaggtat caacgaaatt cataaggtta ctcccggcga tatcagcttt gttgatcatc
     2401 caaaatacta tgataaagcc cttaactcgg ccgctacttt tgttatcatc aacaaagaag
     2461 ttgaatgtcc tgccggaaag ggattgttgt taagcgacga cccgtttcga gattatgtgg
     2521 ctttggttaa acatttttct ccatttaatc cctctttaca acctgttgcg aaaaatgccg
     2581 ttgtgggtga aggtactatt tgctttcccg gtgtatatat cggcaacaat gtgatcatcg
     2641 gaaaaaattg tgtgttgcat ccgaatgtag tgatctatga taattcaatc atcggcgata
     2701 atgtaattat tcatgcaaac acaacaatag gttctgacgc attttatttt aaaaaacgcg
     2761 ctacctattg ggataaatta gtgagctgcg gacgcgtaat tattcacgat gatgttgaaa
     2821 taggttctaa ttgtacaatt gataaaggag tgagtggcga taccattatc ggtcgctcca
     2881 cgaaattcga taatcatatt catatcggtc acggagttgt aatcggtgaa atgtgtttat
     2941 ttgccgcagg ggtaatgata ggtggaaaaa ctattatcgg caatcaggtt acactatggg
     3001 gacaagttgg tgtatccaaa acactcacca ttggaaataa tgtaacagtt cttgcaaaaa
     3061 gcggtgtcga caaagacctc gctccgaata aaatatattg gggtgctcct gcagttgaat
     3121 ctcgccgcgc atggaaacaa cttgctgcag cgaataatat gctggagatt tgggagaaga
     3181 tgaagagggg gtgag
//
LOCUS       DAOQHY010000279         3182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_19914_length_3182_cov_4.043492, whole genome shotgun sequence.
ACCESSION   DAOQHY010000279 DAOQHY010000000
VERSION     DAOQHY010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19914_length_3182_cov_4.043492"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PLL28_14480"
     CDS             complement(<1..573)
                     /locus_tag="PLL28_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70585.1"
                     /translation="MMKKLFALALMSTTIALVYAQSEETSFSATGRGGTATSFVTDYQ
                     AIGINPANLGFETDNAFSLGVAEFGYGLFSNALVKDDVRAILFNNEDSLTSEDQVTLA
                     RSFLNEGITMNVDVMPIGFSLRLPKLGTFGFSIKGNMAYHTLFAGQAANIVFEGYNYE
                     DYIDTIIFDGSTIYGVAYEPLSLGELFDDTE"
     gene            736..1422
                     /locus_tag="PLL28_14485"
     CDS             736..1422
                     /locus_tag="PLL28_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70586.1"
                     /translation="MKTLKSLGIMLLMLCSTALKAQISVEVSEAVSPMSLGERTGYAV
                     TIPRNTEKNAAKAFRAWITEKQKKAEIKETGKHELMVNTFSYAPFGTAPINVYFQFNE
                     GKDGVIVVGFFEVNGAFVSTTTNPDAVATCKQFMTHFAYRMEKIAINEQLEAEKKALD
                     KRNDEQASLEKQEKSLNDKIADCEETIAKAKEDLKTNAGEQETKKEEIKSQSGKVADV
                     EKALKVYDDY"
     gene            1545..2309
                     /locus_tag="PLL28_14490"
     CDS             1545..2309
                     /locus_tag="PLL28_14490"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNF70587.1"
                     /translation="MASSGKKTDNISKQYGITVGEKQFNVFVYLEQRSDCRASIGQKG
                     IHIRLSKYMAEHERFDQEMQLLDWAKKYIAKNGWHERPPLYRKYHDGDTIKVMGKEFT
                     VRIRYVPNKNASNGNLKNGIINLQISEGMSAKQEQKHLSYLVSRLIGNEFQPIIAQRV
                     QVLNDMHFKKPIKKLRLRDNISNWGSCSWDGSINISVRLLFAPPEVIDYILIHELAHL
                     VEHNHSDRFWAQVERAMPGYRLAEDWLHKHGDQCIF"
     gene            complement(2322..2993)
                     /locus_tag="PLL28_14495"
     CDS             complement(2322..2993)
                     /locus_tag="PLL28_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70588.1"
                     /translation="MRSACIGIVGWLLLLFISMQLHAQTAELVGSKLPHGKYLKKDFT
                     IVQGDTIFKKYEKIAFDYTGNDTILFVETYNVKSDWTLSRYIEREGEEEQAGWQEKYD
                     LQGKITYAMFCDTATGDCNVHMQYSYYPNGNLMAQVRYYKHKMDGTSLFYYNDGTFRY
                     HLEYRLGLLWNTLATYDQQGNITDPGDFCDGNGTLNIYAANGVLLKQRIYVDGKVKRT
                     INIAK"
BASE COUNT          994 a          676 c          664 g          848 t
ORIGIN      
        1 ttctgtatcg tcaaataact cgccgaggct gagtggttcg taggctacac cgtaaatagt
       61 gctgccatcg aaaataatgg tgtcgatata atcttcataa ttatatccct caaaaacaat
      121 atttgccgcc tgtcccgcaa acagggtgtg atatgccata ttgcctttga tactgaatcc
      181 gaaggtgccg agttttggta aacgcagact aaatccaata ggcatcacat caacgttcat
      241 ggtgatacct tcattgagaa atgaacgcgc cagtgtcacc tgatcttccg acgtaagact
      301 atcttcatta ttaaataaaa ttgcccgcac atcatctttc acaagggcat tcgagaataa
      361 gccgtatcca aattccgcca cacccagcga aaaagcattg tcagtttcga atcccagatt
      421 ggcgggattg atgccaattg cctgatagtc ggtaacaaac gatgtagccg ttcctccacg
      481 cccggtagcg ctaaacgagg tttcttcaga ttgtgcgtac acaagtgcaa tggtcgtact
      541 catcagggcg agggcaaaca gttttttcat catatcagag gcttatttca ccaaaaatac
      601 ataaaccatt tcaagcggaa atacacatgc cgacattttg aaatacatgc tgtatattag
      661 tgcatgcgcc tgttcaatat taaattggaa tactttttgc atacaggcgg ttcggtcacc
      721 taaaaaatca atgatatgaa aaccttaaaa tctttaggaa taatgttgtt aatgctgtgt
      781 tccactgcgc tgaaagcaca gatttccgtt gaagtaagtg aagcagtttc acccatgagc
      841 ctgggcgaaa gaaccggtta tgccgttact attcctcgca acaccgaaaa aaatgcagca
      901 aaagcattcc gcgcctggat caccgaaaaa caaaagaaag ccgaaataaa agaaaccggt
      961 aaacacgagt taatggtaaa caccttctct tatgcccctt tcggaacagc gccaataaat
     1021 gtgtatttcc aattcaacga aggcaaagac ggtgtaatcg ttgtcggatt ttttgaagtg
     1081 aatggcgcct tcgtttccac tactacaaat cctgatgccg ttgctacgtg caaacaattt
     1141 atgacgcatt ttgcttaccg catggagaag attgccatca acgagcaact ggaagctgag
     1201 aaaaaagccc tcgacaaacg caatgatgaa caggcatctc ttgaaaagca ggaaaaatca
     1261 ttgaatgata aaattgccga ttgcgaagag accatcgcca aggccaagga agacttaaaa
     1321 accaacgctg gtgaacaaga gacaaagaag gaagaaatta aatcgcaaag cggcaaggta
     1381 gctgacgtag aaaaggcgtt aaaggtatac gacgactatt gatagagtcc tgaaataggc
     1441 gcaaacttgt catcacccca aaaggaattg aggtatacat tcctgcaata attttgactt
     1501 gccgaaggga actgtaatct ttgtattgtt gtagtaatca ggcaatggcg tcgagcggga
     1561 aaaagacaga caatatttcg aaacaatacg gaattaccgt tggagaaaaa caattcaatg
     1621 tatttgttta tctcgaacag cgcagcgatt gcagggccag tatcggacaa aaaggcatcc
     1681 atatccgctt gtcgaaatac atggccgagc acgagcgatt tgatcaggag atgcagttgc
     1741 tggattgggc gaaaaaatac atcgcaaaaa acggttggca tgagcgtccg cctctttacc
     1801 gtaaatatca tgatggcgat accattaaag tgatgggcaa ggaattcacc gtgcgcattc
     1861 gttacgtgcc aaacaaaaat gccagcaatg gtaatttgaa gaacggcatc attaatttac
     1921 agatcagcga aggcatgtcg gcaaaacaag aacagaaaca tctcagttat ctggtgagtc
     1981 ggttgatagg caatgaattt caacccatta ttgcgcaacg cgtgcaggtg ttgaacgaca
     2041 tgcacttcaa aaagccgata aaaaaactgc ggcttcgcga taatatttct aactggggaa
     2101 gttgttcatg ggatggcagt attaatattt ctgtacgcct gttattcgca ccacctgaag
     2161 tgatcgatta tatattaatt catgaactgg cacatctcgt agaacacaat cacagtgatc
     2221 gtttctgggc gcaggtagaa cgtgcaatgc cgggttacag actggcggag gactggctgc
     2281 acaaacatgg agatcagtgc attttttgag ttttttattt actacttcgc gatatttatt
     2341 gttcgcttta ctttaccgtc aacataaata cgttgcttaa gtaatacacc attcgctgca
     2401 taaatattta acgtaccatt gccatcgcaa aaatctccgg ggtcggtgat attgccctgc
     2461 tggtcgtagg tagctaatgt gttccacaac agaccaagac gatattcgag atgatatctg
     2521 aaggtgccat cattatagta gaataaacta gtgccatcca ttttatgctt gtaataacgc
     2581 acttgtgcca ttaaattacc attgggataa taactgtatt gcatatgcac attacaatcg
     2641 cccgtggctg tatcgcaaaa catggcgtaa gtaatctttc cttgcaggtc atatttttcc
     2701 tgccatccgg cctgctcttc ttcaccttca cgttcaatat atctactcag cgtccaatca
     2761 gattttacat tgtaggtttc gacaaataat atcgtatcat tacctgtata atcgaaggcg
     2821 attttttcat actttttaaa aatcgtatcg ccctgcacga tagtgaaatc ttttttcaga
     2881 tattttccat gaggtaattt actgccaacc aactcagcag tctgagcatg caactgcatt
     2941 gaaataaata atagcagcag ccatccaacg attccgatgc atgcactgcg catattcacg
     3001 gtatcagaat tttgggcaag gtattttgcg cgctttttta ggcaacgcat cgatataagt
     3061 cagagatatt tcaggcccgc cattagtatt actggcatca gagagcgaag agatattaat
     3121 atcgtaacta acacctaaac ttatttttcc ataatccact cgcgcggcaa tggcggtaga
     3181 tt
//
LOCUS       DAOQHY010000280         3170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_20037_length_3170_cov_6.840449, whole genome shotgun sequence.
ACCESSION   DAOQHY010000280 DAOQHY010000000
VERSION     DAOQHY010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20037_length_3170_cov_6.840449"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="PLL28_14500"
     CDS             <1..922
                     /locus_tag="PLL28_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HNF70589.1"
                     /translation="GTFSHRHAVLREENTNAEYIRLNHLAEKQGKFRIFNSLLSEFGV
                     LGFEFGYSLASPDHLVIWEAQFGDFNNGAQTIIDQFIAASVSKWQRMSGLVMLLPHGY
                     EGQGPEHSSARLERFLQLCAEGNIIVTNITTPANFFHALRRQLAFDFRRPMVNMSPKS
                     LLRHPMAQSQVKELTEGKFHEVIEDAIDPKHVKRVVLCSGKLYYDLLDYRQKNNRNDV
                     ALVRIEQFYPFPETQLERVLAAYSGAEVFWAQEEPRNMGAWYFMQEMMGRRISEAITR
                     KPSASPATGFAKIHAREQQEIVEQAFGTLN"
     gene            935..2143
                     /gene="odhB"
                     /locus_tag="PLL28_14505"
     CDS             935..2143
                     /gene="odhB"
                     /locus_tag="PLL28_14505"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HNF70590.1"
                     /translation="MIISLKVPTIGESINEVTLSRWLVKDGDIVALDQSLCEFESDKA
                     TLEFPSEKSGKIKIIAQEGADLKIGDEIATLDTDFAVAAAVKTEAPVKEESPVAPQEV
                     KKELHVTPLAEKIAEENKVDVSKVPGTGTGGRITKDDVLRFIKEGASPKEIIAARKQN
                     SRNQTQEKVSKLRKTIAKRLVAVKNETAMLTTFNEVDMSAVMAVREKYKAAFEKKYGV
                     GLGFMSFFVRACCNALQEFPAVNAYFNEEQVTFNDFCDISIAVSTPKGLVVPVLRNAE
                     SMSMAEVELKIKELATKGREGTLALEEMQGGSFTITNGGVFGSLMSTPIINAPQSAIL
                     GMHKIQERPIAFNGQVVIRPMMYLALSYDHRIIDGKESVSFLVRVKEQLEKPEQLLFG
                     MDPVGLLLEM"
     gene            2458..>3170
                     /locus_tag="PLL28_14510"
     CDS             2458..>3170
                     /locus_tag="PLL28_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70591.1"
                     /translation="MLISTLLCISFVSVDGQVMTGPGVQWMAEHWYDDNGTIIQDDSG
                     EDWYYDIKPYFDETDNFVGYVAAGFSDIVNETLPGAECLSCGENEVLCYFFETEAHQM
                     GCSRPKLAEMDLLGDVEWYYLYGPENSKGHLRSVVQTESGYVAVGQLKFEGGEGIPYN
                     YEIGSTGEELTLCTEPELDGKPYTVLYLVQTDMEGEDPIERVYGWASPEDAGLYTSEG
                     YQILEDIDGSGYLIIGITEV"
BASE COUNT          957 a          564 c          776 g          873 t
ORIGIN      
        1 aggaacattc agtcaccgac atgctgtatt gcgcgaagaa aataccaatg cagaatatat
       61 tcgcttgaat catctggcag aaaaacaagg taaattccgc atcttcaatt ccttgctcag
      121 cgaatttgga gtattgggat ttgaattcgg ctatagcctc gcttcacctg atcatctcgt
      181 gatatgggaa gcgcaattcg gcgatttcaa taatggggca caaaccatca tagaccaatt
      241 cattgcagca tctgtcagta aatggcaacg tatgagcggt ttggtgatgt tgttgccaca
      301 tggttatgaa ggccagggtc ccgaacatag cagtgcgcgt ttagaaagat tcttgcagct
      361 ctgtgcagaa ggaaatatca tcgtgacgaa tattaccaca cctgcaaatt tcttccatgc
      421 attgcgtcgt cagttggcat tcgatttccg caggccaatg gtaaatatga gtccgaaaag
      481 tttgttacgt cacccgatgg cgcagtcgca ggtgaaggaa ttaaccgaag ggaaattcca
      541 tgaggtgatt gaagatgcca tcgaccctaa gcatgtcaaa cgcgttgtgt tgtgcagtgg
      601 taaattatat tacgatctgc tcgactatcg tcaaaaaaat aatcgcaatg atgtggcttt
      661 ggtgcgcatc gagcaatttt atcctttccc tgaaacacaa ttggagcgcg ttttagcagc
      721 atattccggt gcagaggtgt tttgggcgca ggaagaacct cgaaatatgg gcgcatggta
      781 ttttatgcag gaaatgatgg gcaggcgtat ttcggaagct atcacacgca aaccgagtgc
      841 atcacctgca acaggatttg caaaaattca tgcacgagag caacaagaaa tagttgaaca
      901 agcttttgga acacttaatt aatcttttca gatcatgatt atatcattaa aagtaccaac
      961 aatcggagaa tccatcaatg aagttacgct ttcacgctgg ctggtaaagg atggcgatat
     1021 tgttgcgctt gatcaatcgc tttgtgagtt cgagagtgat aaagctacac tcgaatttcc
     1081 ttctgaaaaa tcaggcaaga tcaagatcat tgctcaagaa ggtgctgatc tgaaaattgg
     1141 cgacgagatc gcaacactgg ataccgattt cgctgtggct gcggctgtta aaacggaagc
     1201 tcctgtaaag gaagaatcac cggttgctcc tcaggaagtg aagaaagaat tgcatgtaac
     1261 tcctttggca gaaaaaattg ctgaagaaaa taaggttgat gtttccaaag taccgggaac
     1321 aggcaccgga ggcaggataa ccaaagacga cgtgttgcgt ttcataaaag aaggtgcttc
     1381 gccgaaagag atcatcgcag cgaggaaaca aaacagtcgc aaccaaactc aggaaaaagt
     1441 aagcaaactg cgcaagacga ttgcgaaaag attagttgct gttaaaaatg agacagccat
     1501 gctcactaca ttcaatgaag tggatatgag tgctgtaatg gccgtgcgag aaaagtataa
     1561 agctgcattc gaaaagaaat atggtgttgg attgggcttc atgagtttct ttgtgcgtgc
     1621 ctgttgcaat gctttgcagg agtttccggc agtaaatgcg tatttcaacg aagagcaggt
     1681 gacattcaac gatttttgcg atatctctat agctgtttca acgccaaaag gattagtagt
     1741 gcctgtattg cgcaatgccg aatctatgag tatggcagag gtggaattaa aaataaaaga
     1801 actcgccaca aaaggacgtg aaggtacact cgcgttggaa gaaatgcagg gaggttcttt
     1861 caccattacc aatggcggtg tatttggatc attgatgagt acaccaatta tcaatgctcc
     1921 gcaaagtgct attttgggta tgcataaaat tcaggagcga cccattgcat ttaatggaca
     1981 agtggtcatt cgtcccatga tgtatctcgc cctcagctac gatcaccgca tcatcgacgg
     2041 caaagaaagt gtaagcttct tagttcgcgt gaaggagcag cttgaaaagc ctgagcaact
     2101 attgtttggg atggatccgg tggggttgtt gttggagatg tgaggtaagg aacaggatca
     2161 gattcaggtg tataagttag atttacttat atatgagtgc aaggtaacta tttccattgg
     2221 tgaaattctc aggtggtctg catcagttag ggtcgccact attttcaaga tgtcatactt
     2281 aaatggatta acggttttcc gtgaatatgt attacaatga gagaaaatta acattaatct
     2341 tttggaagtt atgatttata tggataaatt cgtttatctc attgaaccct aaaacgcaaa
     2401 aatcatgaat tcacaattta ccccccccaa tttaaattaa catttacatt cattaacttg
     2461 ttgatttcca ccttactgtg tatttcattt gtatctgttg acgggcaagt aatgaccggc
     2521 ccgggtgttc aatggatggc agagcactgg tatgatgata atgggaccat cattcaggat
     2581 gattcaggtg aagattggta ttatgatata aaaccctatt ttgatgaaac cgacaacttt
     2641 gtaggatatg tcgctgcagg gtttagtgat attgtaaatg aaactttgcc tggtgcggaa
     2701 tgtctgagtt gtggggagaa tgaagtgttg tgttactttt ttgagaccga agcacatcag
     2761 atgggttgct ctagacctaa attggcagaa atggatttac ttggcgatgt agaatggtac
     2821 tatttatatg gccccgaaaa ctcaaaaggt catttaagat cagtagtgca gactgaatca
     2881 gggtatgtag cagtaggtca gcttaaattc gaagggggag aaggtattcc atataactat
     2941 gaaattggaa gtaccggaga ggaattaact ttatgtactg aaccagagtt agatggaaaa
     3001 ccttatacgg tattatattt ggttcaaaca gatatggagg gtgaagaccc aatagagcgc
     3061 gtgtatggtt gggcctcacc ggaagacgcg ggattatata cttcggaggg ttatcaaatt
     3121 ctggaggata ttgatggtag cggttatttg atcattggta ttaccgaagt
//
LOCUS       DAOQHY010000281         3153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_20213_length_3153_cov_3.877986, whole genome shotgun sequence.
ACCESSION   DAOQHY010000281 DAOQHY010000000
VERSION     DAOQHY010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20213_length_3153_cov_3.877986"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="PLL28_14515"
     CDS             <1..903
                     /locus_tag="PLL28_14515"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNF70592.1"
                     /translation="ATSVIGILQRRFVMRTPLTVPVYPSLIQMRQYELKTMSNISFFE
                     GVKKLRRIGHSYEFDQIKQYVRGDDIRHINWKATGRTGQLMVNHYEEEKSQQVFCIID
                     KSRTMKMPFHDLSLLDYAINTTLTISNIAMRKDDRTGLITFSDKIGTTLSPDKHHAQL
                     RKIMEALYNEKERFTEANYELLYLHVRNFIRVRSLIFLFTNFESLYAIERVVSILRKL
                     NKLHLLVVIFFENAEISNYAREDASNVEEIYLTTIADKFAVEKKQIQRELTKYGIQTI
                     ISRPEDLSINTINKYLELKSRGLI"
     gene            1028..1732
                     /locus_tag="PLL28_14520"
     CDS             1028..1732
                     /locus_tag="PLL28_14520"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNF70593.1"
                     /translation="MNKLILIPVAVMMLATVISCESTQKVQNLKLENASDSLSYAIGY
                     NIGQSFKEQKLTEVDPQLMAVVINAILADDTAGLKMDGTTSVTFIQGYMQKKQEMEDA
                     KAKEEGIKYLEENAKKSGVKVTPSGVQYEVITAGNGKTPVDGDNVTIHYTGTFVDGKE
                     FDSSVRNGQPVTYPVNGFTPGFSEALKMMKEGDKWRVVIPSDLAYGPAGFQGVIPPNA
                     VLIFELELLKVSPATK"
     gene            1880..>3153
                     /locus_tag="PLL28_14525"
     CDS             1880..>3153
                     /locus_tag="PLL28_14525"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNF70594.1"
                     /translation="MRIFTLSLLVAGVITLGQAQPAESGAYSIAISEFNYNSDKFNNS
                     SDWVELHNYGGTAQDLSNWKLKDAIGTTFTIPGGTSLSAGSYLVLVERTDTFAMIHPT
                     VSNYIGNLGFGLDNNSGSIRLFDAGGAVVKQINYLDSVPWPKGSDGYGPSVQILDELA
                     SETDGSNWIAGCVLGTPGSAYVPCDYDIVVSEINYNSLPAYNPGDWIELYNRSTSNIG
                     IGGWTVRDSRNANAFLIPNGTTLNAGARLVITDSLESFNILFAGVTNVIGEPTFNFSN
                     GGDAIRIYEPGDKLKYSIRYKDSAPWPLDPDGTGFTLELVDDSGNPNVAASWVAGCLY
                     GSPGTPFIFPCPDAVTNMHPSDIVVGPVPFTSGLHISLASGNAIDQYKVIDLQGNVVF
                     TQTANAQFAIWDGTNANGTEVPAGIYVISIID"
BASE COUNT          931 a          647 c          689 g          886 t
ORIGIN      
        1 gctacctctg tcattggaat tttacaaaga agatttgtta tgcgcactcc actgacggtt
       61 cctgtgtatc cttcactgat acaaatgcgt cagtatgagc tgaagaccat gagcaatatc
      121 agtttttttg aaggcgtgaa aaaacttcgc cgcattggtc acagctatga gtttgatcaa
      181 atcaaacaat atgttcgcgg cgacgatatc aggcatatca actggaaggc aacaggcaga
      241 accgggcaat taatggttaa tcattacgaa gaggaaaaat cgcagcaagt gttctgcatt
      301 attgataaga gcagaacgat gaagatgcct tttcacgatt tgagtttatt ggattatgcc
      361 atcaacacca cccttaccat cagcaatatt gcgatgcgca aagatgacag aacaggcttg
      421 atcacctttt ctgacaagat cggcactacc ctatcgcccg ataaacatca cgcacaactg
      481 cgcaagatta tggaagcgct gtataatgaa aaagagcgtt tcactgaggc gaattatgaa
      541 ttgttatatc tacacgtgcg aaatttcatt cgcgtacgca gtttaatatt tctatttaca
      601 aattttgaaa gtttatatgc gattgagcgc gttgtatcca ttttgcgtaa gctgaataag
      661 ttacacttat tggtagtgat cttctttgag aatgccgaaa tatcgaatta tgccagagaa
      721 gatgcatcga atgtagagga aatttacctc accactatag cagataaatt tgccgttgag
      781 aagaagcaaa ttcaacgcga actaacgaag tatggcattc aaaccattat ttcacgccct
      841 gaagacttaa gtatcaacac catcaataag tacctcgaat tgaagtcgcg cgggctgatc
      901 tagcactcat ttctaacaag ttttaacgtt tttctaccct tctgcgggcg ttttcaaggg
      961 caatatcagg ttcatgtccc caaaggctca tgcaatatcc ctatctttgc ggcctcaaaa
     1021 tcacaatatg aacaaattaa tcttgattcc cgtagccgtg atgatgttgg caacggtgat
     1081 ctcctgcgaa agcactcaaa aggttcagaa cctcaaactg gaaaatgcat cagattctct
     1141 cagctatgcc attggttata acattggtca gagttttaag gagcaaaaat tgacagaagt
     1201 agatccacag ttaatggcag ttgtgatcaa cgctattttg gctgatgaca ctgcaggctt
     1261 gaaaatggac ggtaccacct ctgttacttt cattcaaggc tatatgcaga aaaaacaaga
     1321 gatggaagat gcgaaggcta aagaagaagg catcaaatac cttgaggaaa acgcaaagaa
     1381 atcaggtgtt aaggttaccc ctagtggagt tcaatatgaa gtgattacag caggtaacgg
     1441 taaaacaccg gttgatggcg acaatgttac tattcactat accggcacct ttgttgacgg
     1501 caaggaattc gatagttctg ttcgcaacgg tcagcctgta acctatcctg taaacggttt
     1561 cacccccgga ttcagcgaag cattgaaaat gatgaaggaa ggcgacaaat ggcgtgtggt
     1621 gatcccttca gacctcgctt atggtcctgc aggtttccag ggagtgatac ctccaaatgc
     1681 cgtgctcatt tttgaattgg aattgttgaa ggttagccct gcaaccaaat aataatgtca
     1741 aaaatcgtga caagacccgc ctgccttatg gtatgcgggt tttttgttgc tgtcacatca
     1801 aggtcaattt aaacccggag tcccctccga ccgtcctatc acttaacaat ttattaattt
     1861 tagggcaata ttaccattca tgcgcatttt tacattgtct ctcctcgtag ccggagttat
     1921 tactttaggt caggcacaac ctgcggaatc cggtgcttac agcattgcca tctcagaatt
     1981 caattacaat tccgataagt ttaataattc atcagattgg gttgaattac acaattatgg
     2041 tggcacagct caagaccttt ctaattggaa attaaaggat gctataggca ccacgtttac
     2101 tattcccgga ggcaccagct tatctgcggg aagctacctt gttttggtgg aaaggactga
     2161 tacatttgcc atgatacacc ctaccgttag caattatatt ggtaatcttg gttttggact
     2221 ggacaataac agcggaagca ttcgcttgtt tgatgcaggt ggtgctgttg taaaacaaat
     2281 caattacctc gattcggttc cctggccaaa aggttcagat ggatacggcc ccagtgttca
     2341 gattctggat gaactggctt ccgaaacaga cggcagtaac tggatagcag gctgtgtcct
     2401 cggcactccg ggaagcgcat atgtgccatg cgattatgat atcgttgtaa gtgaaattaa
     2461 ctataatagt ttgccggcat ataaccccgg cgactggatt gaattataca atcgaagcac
     2521 atctaatatc ggcataggtg gttggacagt tcgcgattcc cgaaatgcga atgcgtttct
     2581 gataccaaac ggaacaactt taaatgcagg agcaagactt gtaattaccg acagtcttga
     2641 atctttcaat attttatttg ccggtgtaac gaatgttatt ggtgaaccga catttaattt
     2701 ctcaaatggc ggtgatgcaa taagaattta tgagcccgga gataaactga aatacagtat
     2761 acgttacaag gatagtgctc catggccact ggatcctgat ggaacaggtt ttacacttga
     2821 attggttgac gatagcggaa atccaaacgt ggcagcatcg tgggttgcag gttgtttata
     2881 tggatcgccg ggaacgcctt tcattttccc ttgtccggat gcggtaacaa atatgcatcc
     2941 gagtgatatt gtagtaggac cggttccttt cacttcagga ctgcatattt cgcttgcttc
     3001 tggcaatgcg atagatcaat acaaagtaat tgaccttcaa ggtaacgttg tattcactca
     3061 aacagcaaat gcacaatttg ctatttggga tggcactaat gcaaatggaa cggaagttcc
     3121 tgccggcatt tatgtgattt ctattataga taa
//
LOCUS       DAOQHY010000282         3141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_20341_length_3141_cov_6.573234, whole genome shotgun sequence.
ACCESSION   DAOQHY010000282 DAOQHY010000000
VERSION     DAOQHY010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20341_length_3141_cov_6.573234"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..400)
                     /locus_tag="PLL28_14530"
     CDS             complement(50..400)
                     /locus_tag="PLL28_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70595.1"
                     /translation="MKLKNLLGLATGIILMCQSDLVNAQLYTYTNDETGVAGTVATGV
                     TASNLTPVNGVVAINACGTGYSSDTWSTSTGAFSTAFSAIQLTLTPTTGYNVTVTAIQ
                     FDLGRNPQGPQRMR"
     gene            650..1552
                     /gene="gldA"
                     /locus_tag="PLL28_14535"
     CDS             650..1552
                     /gene="gldA"
                     /locus_tag="PLL28_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HNF70596.1"
                     /translation="MSVKVQNITKIYGEQRAVDDISFTVSPGEILGFLGPNGAGKSTT
                     MKILTCFIPQTGGEASVCGFDINKQAVEVRRQIGYLPEHNPLYKEMYVQEYLEFAANI
                     NGMKKASARINEMIEITGLVPERKKKIGQLSKGYRQRVGLAQAMLHDPKVLILDEPTS
                     GLDPNQLVEVRNLIKNIGKDKTVILSTHIMQEVTAICNRVIIINKGKLVADDSISSLQ
                     SRQKGITVVLAEFKETVDKGMLMRINGVSKVAAEGKALRIEYTGREDMRELLFKFAVE
                     HKLTLLNLQIETKSLEDIFHTLTK"
     gene            1594..2325
                     /gene="gldF"
                     /locus_tag="PLL28_14540"
     CDS             1594..2325
                     /gene="gldF"
                     /locus_tag="PLL28_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HNF70597.1"
                     /translation="MFAIFKKELNVFFSSLIGYIAITIFLVANGLFTWVFPETNILDE
                     GFATLDAFFNFAPWILMFLIPAITMRSFAEEKSSGTIEFITTRPVTDIQIILGKYLGA
                     FVLVIFSIIPTITYYFAIRGLASPVGNVDTGGIIGSYIGLILLGACFVAIALFTSAVT
                     NNQIVAFISGMFACFFFYLAFDSLSLIPVFVGSADSYFNALSINQHYQNISKGYIDLR
                     DVVYFFSMITIFILMTKTALGSRKW"
     gene            2336..>3141
                     /locus_tag="PLL28_14545"
     CDS             2336..>3141
                     /locus_tag="PLL28_14545"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HNF70598.1"
                     /translation="MDAKLLYKKNRRRELLQLLLLTAIIVIVNVVVSGVFFRIDLTQE
                     KRYSLSTPTKELLRAQDDIILARVYLAGNLTPNLQRLQQSTVEMLDQFKNYAGENIQY
                     DIFDPFSIPDETNQTELIKELEDKGILSVQFFESATDEASVKYVFPFISLTYRDRELV
                     FPLIDRGTMPLPLNPDSDPSVSISLLEYKFTKAIRQLTTEEKPYIAFISGHGELNRLE
                     LNDMAASLNELYNVTGIDLEDDSTYEIPRQIKTVIIAKPLTAFSVKGKFIL"
BASE COUNT          942 a          587 c          690 g          922 t
ORIGIN      
        1 tttttattta agtgttctgt ctatttttgg gtgtctatgc atattgcagc taacgcatgc
       61 gttgaggccc ctgagggttg cgaccaaggt caaactggat agctgtaaca gtaacattat
      121 atccggtagt tggggtcaat gtcaattgaa tagctgaaaa agcagtagaa aatgcacctg
      181 ttgatgtgct ccaggtgtca gagctgtaac ctgttccaca ggcgttgatg gcaacaacac
      241 cattaaccgg tgtaaggtta gaagcagtta cacctgttgc aacagttccg gcaacaccgg
      301 tctcgtcgtt ggtgtatgtg taaagctgcg catttactag atcagactga cacatgagga
      361 tgatacctgt agccagccca aggagattct ttaatttcat tttgtatgct tttttggtta
      421 gtgcgggcaa aggtaggacg ccgagtaaga ttattcacct aaaaggtgat aaaatgtaag
      481 gaattgatga ctttgcgaca atatttaaca tattccggcc attggcaatt ctataaacag
      541 atgagccttt ggagatttat gcatcctata aaataggcaa taaagtattt tacatgttta
      601 caatgccagg acttaaacct tcgtttgatt acctaatttt gcggctttca tgtcagtaaa
      661 ggttcaaaac atcaccaaga tctacggcga gcagcgtgcg gttgacgata tctcatttac
      721 ggtgagtccg ggcgaaatac ttggcttttt agggccgaat ggtgccggga agtccactac
      781 catgaaaatt ctcacctgtt ttatccctca aaccggcggt gaagccagtg tttgcggatt
      841 tgatatcaat aagcaagctg tagaagtgcg caggcaaata ggctatttgc cggaacataa
      901 tccattatat aaggagatgt atgtgcagga atatctggag ttcgctgcca atatcaatgg
      961 catgaagaaa gcatctgcca ggattaacga gatgatagag ataacaggat tggttcctga
     1021 gcgaaagaaa aaaataggtc agctatcgaa aggatatcgt cagcgtgtgg gattggcgca
     1081 agccatgtta catgatccga aggtgttgat tctggatgaa cctacttcag gtttagaccc
     1141 gaatcagttg gttgaagtac gcaatctcat taaaaatatc ggtaaggata aaacggtaat
     1201 tctttccacg catatcatgc aggaggtaac tgccatttgc aatcgcgtaa tcatcatcaa
     1261 caaaggaaag cttgttgccg atgacagtat cagcagttta caatccaggc aaaaaggcat
     1321 tactgttgtg ttggctgaat ttaaagaaac ggttgacaag ggtatgctta tgcgcataaa
     1381 tggtgtgagc aaagtagcag cagaaggaaa ggctttgcgc atagaatata ccggacgcga
     1441 agatatgcgc gaactgcttt tcaaatttgc agttgagcat aaactcacgc tattgaattt
     1501 acaaattgaa acaaaaagtc tggaagacat attccatacg cttacaaaat aatttaagta
     1561 cttgtatact cagcataaaa tttaaaataa acagtgtttg ccattttcaa aaaggaatta
     1621 aatgtattct tcagttcgct gataggatat attgctatta ccattttttt ggtggcaaac
     1681 ggattattta cttgggtgtt tcccgagact aatattttag acgaaggttt tgccacgctt
     1741 gatgcatttt tcaacttcgc gccatggatt ctgatgtttc tgataccggc catcaccatg
     1801 cgttcatttg ccgaagaaaa gagcagcggc accatagaat ttattactac aagaccagtc
     1861 actgatattc aaataatatt aggtaaatat ttaggtgcat ttgtattggt tatattcagt
     1921 atcataccaa ccatcaccta ttacttcgcc attcggggtt tggcgtctcc ggttggaaat
     1981 gtggataccg gaggtattat tggaagttat atcggattaa tactattagg cgcatgtttt
     2041 gttgcgattg cgctatttac atctgcagtt accaataatc agatagttgc atttatctcc
     2101 ggcatgttcg cttgcttctt tttctatctg gcgtttgaca gtttgtcgct cataccggta
     2161 tttgtcgggt cggcagatag ttatttcaat gcattgagta taaatcaaca ttatcaaaat
     2221 atcagcaagg gttatatcga cctgcgcgat gtggtatatt tcttcagtat gatcaccatt
     2281 ttcatattga tgacaaaaac agcattggga agcagaaaat ggtaattgat tgaacgtgga
     2341 cgcgaagctt ttatataaaa agaacagaag acgggagttg ctgcaactac tgctgcttac
     2401 tgctataatt gtaatcgtca atgtggtggt ttcaggggta ttttttcgca tagatcttac
     2461 tcaggaaaaa cgctattcac ttagcacccc tacaaaagaa ctgctgcgcg cccaagatga
     2521 tattattttg gcacgagttt atcttgcggg caatctcaca cccaatctgc aacgattaca
     2581 gcagtcgaca gtagaaatgc tcgatcagtt taagaattat gcgggagaga atattcaata
     2641 cgacattttt gatccgttca gcataccgga tgaaaccaat caaactgaac tgatcaaaga
     2701 actggaagat aaaggaatat tgagtgttca gttttttgaa agcgcgacag atgaagcatc
     2761 agtgaaatat gtattccctt ttatttcgct cacctatcgc gacagggaat tggtatttcc
     2821 tcttattgac agaggcacca tgccattgcc tctgaacccc gatagtgatc catcggtttc
     2881 gatatcatta ctggaataca aattcacgaa agcaatccga caacttacca ccgaagagaa
     2941 accatatatc gcatttattt cagggcatgg agaattgaat cgcctggagt tgaacgacat
     3001 ggccgcttcg cttaatgaat tatacaatgt tacgggcatt gatcttgaag atgacagcac
     3061 ttatgagatc ccgcgtcaaa taaaaactgt cattattgcg aaaccgctga cagctttttc
     3121 agtaaaaggg aaatttattc t
//
LOCUS       DAOQHY010000283         3025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_21643_length_3025_cov_5.110101, whole genome shotgun sequence.
ACCESSION   DAOQHY010000283 DAOQHY010000000
VERSION     DAOQHY010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21643_length_3025_cov_5.110101"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PLL28_14550"
     CDS             complement(<1..301)
                     /locus_tag="PLL28_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70599.1"
                     /translation="MKFNTAIVLACTIFAISATSCKQDEVPEVTYDVTFTIIEPEADF
                     VAVSGDELHMEVDLQGTKAIGNAEMLLIDEMSGDTLFTYSTTTTSDFLTMHEHYIP"
     gene            complement(360..734)
                     /locus_tag="PLL28_14555"
     CDS             complement(360..734)
                     /locus_tag="PLL28_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70600.1"
                     /translation="MKRIVIIILTFAYLSGICSPFYTYVGYLINKDYIAANLCENKDK
                     PEMNCEGKCQLTVQLAKDVEQKQDNKAPQSGNRELNPHTIASFNFTPYIGVISVFAEN
                     RSQQQFADMYIAAPESPPRLFV"
     gene            complement(855..1406)
                     /locus_tag="PLL28_14560"
     CDS             complement(855..1406)
                     /locus_tag="PLL28_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HNF70601.1"
                     /translation="MKKAAFYLLLVTFSFSASMAYAQTTTVGGEAMYPQKDIVDNAVN
                     SKDHTTLVAAVKAAGLVETLKSAGPFTVFAPVNDAFENLPDGTVATLLKPENKDMLTK
                     VLTYHVVAGKLDYAAIEKMIKDGKGTAELTTVSGGKLWAMMNGKHNIQLKDESGNLIN
                     ISTYDVYQSNGVIHVIDHVALPK"
     gene            complement(1556..2218)
                     /gene="phoU"
                     /locus_tag="PLL28_14565"
     CDS             complement(1556..2218)
                     /gene="phoU"
                     /locus_tag="PLL28_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNF70602.1"
                     /translation="MSQIDNPIHELKHSLEQMFQLVSTQLEKSKEALSKFDNDLAREV
                     IQIEKRVNAQELQIDRVAENFLALYSPVAIDLRFVLAVLKINNNLERIGDIAEGISKY
                     LNETERPLDAKLMEESNVIPMYEAAVRMLSDVHEAFEKEDTTIARKIFKSDDYLDHIN
                     LEAINNLTEAMAGDASKIQDALYLISIIRKLERIGDQLKNIAEEIIFYIEAKVLKHAK
                     QK"
     gene            complement(2248..3003)
                     /gene="pstB"
                     /locus_tag="PLL28_14570"
     CDS             complement(2248..3003)
                     /gene="pstB"
                     /locus_tag="PLL28_14570"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNF70603.1"
                     /translation="MSNKLTAHEVHVWYGSFHALKNISLTIKPNTVTAFIGPSGCGKS
                     TFLRLFNRMNDYIDGFHMGGEITIDGRNIYGKRVMVEELRKEVGMVFQKPNPFPKSIY
                     DNVVYGLRIQGINDKHLLEEACETSLRHAALWDEVKDDLKKSALALSGGQQQRLCIAR
                     TLAVKPTMILMDEPASALDPISTAKIEELILQLKDDYTIAIVTHNMQQAARISDKTAF
                     FYLGELIEYDKTVNIFTNPKEEKTQSYITGRFG"
BASE COUNT          859 a          625 c          585 g          956 t
ORIGIN      
        1 acggaatata atgctcatgc atggtcagga aatctgacgt agtggtggtt gaataagtaa
       61 acaaagtatc gcctgacatt tcatctatga gtaacatctc ggcattgcca attgcttttg
      121 ttccttgcaa gtcgacttcc atgtgcaatt catcacccga cacggcaaca aaatcggctt
      181 caggttcaat aatggtgaat gttacatcat aagtaacttc aggaacttca tcttgtttac
      241 atgaagtagc agaaatagcg aatatggtac aagctaatac aatagcagta ttaaatttca
      301 taaaaattta gtttgtgcaa ttaaataata aattcaagtg cgttggcaca gggttgctat
      361 tacacaaaca aacgtggagg agactccggc gcggcgatat acatatcggc aaactgctgt
      421 tgcgaacggt tttccgcaaa aactgaaatt acgcctatat aaggagtaaa attaaacgat
      481 gcgatggtgt gaggatttaa ctcacgattg cctgattgag gcgctttatt atcctgcttc
      541 tgctcaacat cttttgccaa ctgcacggta agctgacact ttccttcgca gttcatttcc
      601 ggcttatcct tgttttcaca aaggtttgca gcgatataat ctttattgat gaggtaacct
      661 acatacgtat aaaatggcga gcagatgccc gacaagtatg caaaggtcaa tatgatgata
      721 acaatgcgtt tcacctcagg gtagtagtgc aaagatacat actcacctat tcaggtttat
      781 gactttggtc accatttaaa aaaaattagg gatcgaccct caggccgacc cctaacgtta
      841 caaaacatca gaacttactt aggaagtgcc acatggtcga tcacatggat cacgccattg
      901 ctttgataca catcgtaggt gctgatattg atgaggttac cgctctcatc tttcaattga
      961 atattgtgct taccattcat catggcccaa agtttaccgc cacttacagt agtaagctca
     1021 gctgttcctt tgccatcctt gatcattttt tcaattgctg cataatccaa tttacctgct
     1081 acaacatggt aagtgagcac ttttgttagc atgtctttgt tttcaggttt tagcagggtt
     1141 gctacggtgc cgtcggggag attttcgaag gcatcattta ccggtgcgaa aaccgtaaat
     1201 gggcctgccg actttaacgt ctcaaccaac cctgctgctt tcacagctgc taccagtgta
     1261 gtatggtctt tagaattaac ggcattatct actatgtctt tttgaggata catggcttca
     1321 ccgccaacag tagtggtttg cgcatatgcc attgaagcgc taaaagaaaa ggttaccaat
     1381 aaaaggtaaa atgctgcttt tttcatgatt ttagtttttg tatgcagcta tttacgcatg
     1441 acagttcacg catggtttta aaaaatcaaa aaaaatattt ttcaaaaaac gatgcgtata
     1501 aaaaaccaaa acccggctac tttcgtaacc gggtttcttg aatccgaatc gttaattatt
     1561 tctgtttcgc gtgcttgagc acttttgctt ctatgtaaaa tatgatctct tcagcaatat
     1621 ttttcaattg atcgccgata cgttccagtt tacggatgat actgatgaga tacaaggcat
     1681 cctgtatttt agatgcatct ccagccatgg cttctgtgag gttattaatg gcttcgagat
     1741 taatgtgatc gagataatcg tcgctcttga atatttttct tgcgatggta gtatcctcct
     1801 tttcaaaagc ctcatgtaca tctgataaca tgcgcactgc agcttcatac atgggaatga
     1861 cattgctttc ctccatcagt ttggcatcta aaggcctttc agtttcattc agatatttag
     1921 aaatgccctc tgcgatatcg ccgatgcgct cgaggttgtt gttgattttt agaacagcaa
     1981 gcacaaatcg caggtcaatg gctacaggag aatacaatgc gaggaaattc tcagccacac
     2041 gatcgatctg caattcctga gcattaacgc gcttttcaat ttgaattact tcacgtgcaa
     2101 ggtcattatc aaatttagaa agagcttcct ttgacttctc caattgtgtt gaaaccaact
     2161 gaaacatttg ttccagcgaa tgctttaact catgtatcgg attatctatt tgagacatag
     2221 taatgaaatt aatggtaata aaaaatatca accgaaacgg ccggtgatat agctttgagt
     2281 tttttcttct ttaggatttg tgaagatgtt aactgtttta tcgtactcta tcaattctcc
     2341 caggtagaaa aatgctgttt tgtcgctgat acgtgccgct tgttgcatat tgtgtgttac
     2401 aattgcgatg gtgtaatcat ccttcaattg caaaatcagc tcttcaatct tggcggttga
     2461 aatgggatcc aatgcacttg ccggttcatc catcaaaatc atagttggct taacagcaag
     2521 tgtacgagca atacacaaac gttgctgttg tccacccgat aaggccagtg ccgatttttt
     2581 taagtcgtcc ttcacctcat cccaaagtgc ggcatggcgc aatgatgttt cacaagcttc
     2641 ttctagcaga tgtttatcgt ttataccttg aatgcgcaat ccgtaaacaa cgttatcata
     2701 aattgattta gggaagggat ttggtttttg gaacaccatg cctacctcct ttcgcaattc
     2761 ctctaccatt actcttttgc cataaatatt tctaccgtct atggtgattt ctccgcccat
     2821 atgaaacccg tcgatataat cattcatgcg attaaacaat cggaggaatg tagatttccc
     2881 acaacctgaa ggaccaatga atgcagtaac agtatttggc ttgatggtga gagaaatatt
     2941 tttcagggca tgaaaactgc cataccaaac atgtacttcg tgtgctgtta atttattact
     3001 catattccac ttaccatttt acttt
//
LOCUS       DAOQHY010000284         3007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_21872_length_3007_cov_5.306233, whole genome shotgun sequence.
ACCESSION   DAOQHY010000284 DAOQHY010000000
VERSION     DAOQHY010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21872_length_3007_cov_5.306233"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PLL28_14575"
     CDS             complement(<1..422)
                     /locus_tag="PLL28_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70604.1"
                     /translation="MFCKNVAFLIAFCLLSLFTQQSLSAQSYAISGIVIDETSGLGLQ
                     DVVVTINNQQIISDKRGNFITQSNSKQLIIDCSMIGYSLINIPVIQIDTQAHKIDITI
                     KLTRNLIELPNFEITAQRVATTMPVTHTNIYASQLEQI"
     regulatory      complement(471..572)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(796..1689)
                     /locus_tag="PLL28_14580"
     CDS             complement(796..1689)
                     /locus_tag="PLL28_14580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70605.1"
                     /translation="MKPFKQFTLQQYSGLAGALLFLHGNTSAQAVYTDIEPDVTLQYD
                     DDYVYIDLDGDGSNDYFLLKDSHYFSNTFYEIYRFNHVLYCGPNFNPENSIAGTFATN
                     GEGGGTTYFPYALNSGELINSDLNFQYGHYQLMAIGFYKISVTPWEWHYGIGYWTPNK
                     DSLFLGVRFLGGDDCKHYGWIRCSTADSAKTLIVHDFAYETKCETPIVAGDTIGDTTT
                     VVVNNLLELNAEIFAYNMQLFVRSNVAHQIAIFDSSGKKILDNSRQQGTYNIDMQPFP
                     KGIYIVELTTGSQMLTKIVSL"
     gene            complement(1686..>3007)
                     /locus_tag="PLL28_14585"
     CDS             complement(1686..>3007)
                     /locus_tag="PLL28_14585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70606.1"
                     /translation="PNSGIAGGCVTEDCDDWTNDQWQFDTIDERLSNAYKAVIGLGSN
                     GFGNFKQFIRTRYAESYDQQRLDLMLYGNEPWKKDLWTTEWNLKDKNTDYLQGSMEQN
                     SLTSFVNSFEHGWLIHEWFLKNIKQNYAKNYRKGFHTYSMFHAYGGGSFYALLLQSDR
                     ADREKHLDELGNPDTLTSPPANQNLFLKRTMYHTFDMLSVIKSNNLKYLPSNFTAYAH
                     NPNIQPTVFIDKSNKKLYIYYSNMKTETQSYVLEKGNILMLYPGSTAMAYGEAFMYNI
                     APTQLYSNSGNSFLYKMNICYNTTDQQHPFEIQYINGPVINEPECSGLPGGAICLTVP
                     ANSYGYIEVPFYITPREGLMLSEDQIEVYPNPTANTFRIACDIPEELLNTFYVSVLDI
                     NGKLLLNTVASQNKDIDLGNLPSNIYFIVISNKEKSFSVTKKIIKAE"
BASE COUNT          895 a          565 c          562 g          985 t
ORIGIN      
        1 tttatctgtt ctagttgcga agcgtagata ttagtatggg taaccggcat agttgtagcc
       61 acacgttgtg ccgtgatctc gaaattaggc agctctatta agttcctggt aagctttatg
      121 gtgatatcaa ttttatgcgc ctgggtgtcg atttgaataa caggaatatt aattaacgaa
      181 tatccaatca ttgagcagtc aattatcaat tgcttactat tcgattgagt aataaaattc
      241 ccgcgcttat ctgatataat ctgttggttg tttattgtaa ctacaacatc ctgcaagcct
      301 agtccgcttg tttcatctat tacaattccg gatatcgcat acgattgagc cgatagcgat
      361 tgttgtgtaa acaaactcag caaacaaaaa gcaattaaaa aagctacgtt tttacaaaac
      421 atggcaaagg tttacatttt gaatcctgta aatggagcct ttaccggtaa gaatactaat
      481 ttcctctatc ccttcccggc attacccggg caggttcgat gggtttaatc tcagtcgttc
      541 agaaaatctg tcggacaccc catgagatat gcaaagatac tagaattcat ttaataatac
      601 cattggcatc tttcctgcgt tgatgcctac taccgtccta taaataagtg gcccttataa
      661 cttaggttag actagcttac tgcaagcata gtgggacggg ttgcttaatt gccttgaata
      721 aaatgggtat acttgaaacc tataactcta acatatttct gagatggaat cggattctat
      781 caataacata cctcgtcata aagatacgat cttagtcagc atttgcgatc cggttgtcaa
      841 ttctacgatg taaattcctt ttgggaaagg ttgcatgtcg atattgtatg tgccttgttg
      901 ccgactgtta tcaagtattt tcttgccgct gctgtcaaat attgctattt gatgggcgac
      961 attacttcta acaaacaatt gcatgttgta agcaaatatt tctgcattta attccaataa
     1021 attgttcaca acaactgtag tagtgtcgcc aatggtatcg ccggcaacaa ttggcgtttc
     1081 gcattttgtt tcataagcaa aatcgtgcac aatcaatgtc ttggcagaat cagctgttga
     1141 gcacctaatc catccataat gtttgcaatc gtcaccgccg agaaaacgga cgcctagaaa
     1201 cagtgagtct ttatttggtg tccaatagcc tatcccataa tgccattccc aaggtgttac
     1261 actaatttta taaaatccaa ttgccatcaa ctgataatgt ccatattgaa aattcagatc
     1321 actattgatt aattctccgg agttaagggc ataggggaaa taggtagttc ctccgccttc
     1381 accgttggtg gcaaatgtac ccgctatgct attttcagga ttaaaatttg gaccgcaata
     1441 taggacatga ttaaatctat aaatttcata aaatgtatta gaaaagtaat gactatcttt
     1501 taataaaaaa taatcattgc ttccatcgcc atccaggtca atatatacat aatcatcatc
     1561 atattgcaat gtaacatccg gttctatatc agtataaaca gcctgtgccg acgtattacc
     1621 atgcaagaat aacaatgctc ccgcaaggcc tgagtattgt tgcaatgtaa attgcttgaa
     1681 gggtttcatt cagctttgat gatttttttt gttactgaaa agcttttttc tttattgctg
     1741 ataacaataa aatagatatt tgaaggtagg ttgcctaagt caatatcttt attttgagag
     1801 gctacagtat ttaataatag tttgccgttt atatctaaaa ctgatacata gaatgtgttg
     1861 agcaattctt caggtatatc acatgcaatt cgaaaggtat ttgcagttgg attgggatac
     1921 acctcaattt gatcctctga cagcatgagc ccttctcgcg gagtgatata aaatggcact
     1981 tcaatataac cataagaatt tgcagggact gtaaggcaaa tggcacctcc cggtaaacct
     2041 gagcattcag gttcattgat aactgggcca ttgatgtatt ggatctcgaa cggatgctgt
     2101 tgatccgtag tattgtagca gatattcatt ttatataaaa aactattacc gctattggaa
     2161 tagagctgtg taggagcaat attatacata aatgcctcac cataggccat agcggtagaa
     2221 cccggataca gcatcaagat attacctttt tccaaaacat agctttgtgt ttcggttttc
     2281 atattggaat agtaaatata cagcttttta tttgatttgt caataaaaac agttggttgt
     2341 atattgggat tatgcgcata agcggtaaaa ttcgaaggca ggtacttaag attgttactt
     2401 tttattacac tgagcatatc aaatgtatgg tacattgtac gtttcaaaaa taaattttga
     2461 ttggcaggcg gtgaagttaa ggtgtccgga ttgccgagtt catcaagatg tttttctcga
     2521 tctgccctgt ctgattgtaa aagcaaggcg taaaatgacc ctccaccata agcgtgaaac
     2581 attgaataag tatgaaagcc ttttcgatag ttcttagcat agttttgttt aatgtttttc
     2641 aggaaccact catgaataag ccagccatgc tcaaatgagt taacaaatga agttagtgag
     2701 ttttgctcca ttgagccttg taggtaatca gtatttttat ctttaaggtt ccattctgtt
     2761 gtccataaat cttttttcca cggttcattc ccataaagca tcaagtcgag cctctgttga
     2821 tcgtaagatt cagcgtacct tgttcgaata aactgtttaa agttcccgaa tccatttgaa
     2881 cccaatccta ttacagcttt ataagcattg cttaggcgtt cgtcaattgt atcgaattgc
     2941 cactgatcat ttgtccaatc gtcacaatct tctgtaacac atccaccggc tatgcctgaa
     3001 tttggat
//
LOCUS       DAOQHY010000285         2993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_22022_length_2993_cov_4.784888, whole genome shotgun sequence.
ACCESSION   DAOQHY010000285 DAOQHY010000000
VERSION     DAOQHY010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22022_length_2993_cov_4.784888"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="PLL28_14590"
     CDS             <1..750
                     /locus_tag="PLL28_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNF70607.1"
                     /translation="LQLKRAAEMVEGTPGGILVITEGVFGMGGDQGKLKEIVELKKKF
                     NFRLLVDDAHGFGTMGATGAGTGEAQGCQDGIDLYFSTFAKSMASIGAFISGEKEVIW
                     YLRYNLRSQIYAKSLPMPIVIGNIKRLELLRNNPELRERLWDNVRKLQEGYRSRGFNL
                     GNTDSPVTPVFLEVSIPETINLVVDLRENHGIFCSAVTYPVIPKGMLMLRIIPTASHN
                     DDDIQKTLDAFEAIREKIRTGAYLKEDLVKV"
     gene            877..1065
                     /locus_tag="PLL28_14595"
     CDS             877..1065
                     /locus_tag="PLL28_14595"
                     /inference="COORDINATES: protein motif:HMM:NF019076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70608.1"
                     /translation="MELHQKLSRLKAIVSLAEEDLQKFTDKGNKTAGTRLRKSLLEIK
                     EIAGDMRKEVLESKKVKV"
     gene            complement(1205..2125)
                     /gene="dapA"
                     /locus_tag="PLL28_14600"
     CDS             complement(1205..2125)
                     /gene="dapA"
                     /locus_tag="PLL28_14600"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNF70609.1"
                     /translation="MASKYTAATTHRFRGTGVALVTPFLPDGGIDFAALRTLIDHVIE
                     GGVEYVVSMGTTGESATLTEEERFSIIDATIEHVNGRIPVVGGFGGNDTNAIIRSIEK
                     FRAHRSGKSYIDAILIASPYYNKPSQEGIYQHYLAIDKHVTVPIILYNVPGRTSSNME
                     AATTLRIARDAEHVIAIKEASGNFNQCMYIIKDAPEGFLVISGDDGITLPFISMGMQG
                     VISVVANAYPRPFSDMVRLSLDNKFEGANLIHYKLLAFTDLIFKEGSPAGVKAALKHL
                     NICDEKVRLPLWKISEGLEKKIVDEMNSIG"
     gene            complement(2156..2680)
                     /locus_tag="PLL28_14605"
     CDS             complement(2156..2680)
                     /locus_tag="PLL28_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70610.1"
                     /translation="MPSIKNILFGRFIRSKPYTDTMERSAIPYARIKTMAFLVDGSDP
                     IALRELLNYLHRYTEEGKKITILGYVKKLPPFEEEIIWITAKDKNWIGIPKMFRIKHF
                     IEKEFDVLINTAMKSNRPLEYVSTYSKAKLRIGKYDQQKTFAYDFMMQTENEDVFSYL
                     AQVEHYLRMVKTEP"
     gene            complement(2684..>2993)
                     /locus_tag="PLL28_14610"
     CDS             complement(2684..>2993)
                     /locus_tag="PLL28_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HNF70611.1"
                     /translation="ISPESCSSILWRSWDYKEVAAEALKLTAADNYRNKIIDGIVKEP
                     LGGAHTDPEESARLLKKHLKKHLKELIPMDPDERIRLRIEKFDDMGVFDEVTPKGANA
                     "
BASE COUNT          849 a          656 c          603 g          885 t
ORIGIN      
        1 ttgcaactca aacgcgctgc cgaaatggta gaaggcactc cgggtggtat cctcgtgatt
       61 accgaaggtg tgttcggaat gggtggcgat caaggaaagt tgaaggaaat tgttgaattg
      121 aaaaagaaat tcaatttccg cttacttgtt gacgacgctc acggcttcgg aaccatgggt
      181 gctacaggcg caggaacagg tgaagcgcag ggatgtcagg atggtatcga cctctacttc
      241 tctaccttcg ctaaatcaat ggccagtatc ggtgccttca tcagtggcga aaaagaggta
      301 atctggtatc tccgctataa cctgcgttct cagatatatg ctaagtcatt gccgatgcct
      361 atcgttatcg gtaatatcaa acgccttgaa ttattgcgta acaaccccga attgcgtgaa
      421 cgcctgtggg ataatgtgcg caaattacaa gaaggatacc gcagtcgcgg cttcaatctc
      481 ggcaataccg atagccctgt aacgccggta ttccttgaag tttctattcc tgaaacaatc
      541 aacctggttg tcgatttacg tgagaatcat ggtatctttt gttctgctgt aacctatccg
      601 gttatcccga aaggaatgct catgttgcgt atcattccaa cagccagtca taacgacgat
      661 gatattcaaa aaacactgga tgcttttgaa gccatccgtg aaaagattcg cacgggggca
      721 tatttgaaag aagacctggt taaagtctga ccaaaacaaa attaatctta cagccgggta
      781 gaaaattctc ccggcttttt tataatttgc acctccttac accttcaacg ggaacgtgaa
      841 ccccggcgca ggtaaaacta ctaacacact ttgattatgg aactgcatca gaaactcagc
      901 cgcctaaaag caattgtatc cctcgccgaa gaagacctcc aaaaattcac cgacaaagga
      961 aacaaaactg caggcacgag actgcgcaaa tcactgctcg aaataaaaga aattgccggt
     1021 gatatgcgta aggaagttct tgaatctaaa aaagtgaaag tatagcatct ggtatcaagc
     1081 ttgcctcgcc acgggcgggt atctggtatc aagtctcaat ccgctttgcg cctttgtttc
     1141 tttgcgactt agcgcgaaaa aaaaaatcct aatgtagaat tggaatccct aaggtcaata
     1201 cctttcatcc gatactattc atttcatcca caatcttttt ctctaatccc tcagagattt
     1261 tccatagcgg cagacgcacc ttttcatcac aaatattaag atgtttcaat gcagctttta
     1321 cgcccgcagg tgatccttct ttgaatatta aatcggtgaa ggcaagaagt ttatagtgaa
     1381 taagattagc accctcgaat ttattatcaa gcgacaatcg aaccatatcg ctgaatggtc
     1441 tcggataggc atttgccaca acagaaataa ctccttgcat gcccatgctg atgaatggca
     1501 aggtaattcc atcatcacca ctgatcacca aaaatccttc aggtgcatcc ttaatgatat
     1561 acatgcattg attaaaattt ccactggcct ccttaatcgc aatcacatgt tccgcatctc
     1621 gagcaatgcg cagggtagtg gcagcctcca tatttgatga ggtgcgaccc ggaacattgt
     1681 ataaaataat cggaacggta acatgtttat ctattgcaag ataatgctga taaattcctt
     1741 cctgcgatgg tttattatag tatggtgaag caattaatat cgcatcgata tatgattttc
     1801 cgctgcgatg tgcgcgaaat ttttctattg atcggatgat ggcatttgta tcattaccac
     1861 caaatccacc tacaacagga atacgtccat ttacatgttc aatcgttgca tcaataatgg
     1921 aaaagcgttc ctcttccgtt aaggtggcgc tttcgccggt agtgcccatg cttacaacat
     1981 attctacgcc gccttcaatt acgtgatcta taagtgtgcg caatgctgcg aaatcaattc
     2041 ctccatcagg taaaaatggt gtcaccaatg ccactcctgt tccgcgaaag cgatgtgttg
     2101 ttgctgcggt atatttgctt gccatacttg aaataaataa ttgtaatgaa aataattacg
     2161 gttctgtttt taccatgcgc agataatgtt caacttgtgc gaggtaagaa aatacatctt
     2221 cattttctgt ttgcatcatg aaatcatatg caaatgtttt ttgctgatcg tatttcccga
     2281 tgcgcaattt tgctttgctg tatgttgaaa catattccag cggacggttt gatttcatgg
     2341 cagtattgat caatacatca aattcttttt caataaaatg tttgatgcga aacatctttg
     2401 gtataccgat ccagttttta tcttttgctg ttatccagat gatttcttct tcaaatggag
     2461 gcaatttttt tacatatccg aggatggtga tttttttccc ctcttcggta tatctgtgca
     2521 gataattcaa taattcacgc aaagcaatag ggtcactgcc gtccaccaaa aatgccatcg
     2581 tttttatgcg tgcataagga attgcactgc gctccatcgt atcggtgtac ggttttgagc
     2641 gtatgaagcg accaaataat atattcttga tcgagggcat gcgttatgcg ttggcaccct
     2701 tcggtgttac ctcatcgaaa acacccatat cgtcgaattt ctcgatgcgc aagcgaatgc
     2761 gttcatcagg atccattgga atgagttctt tcagatgttt ctttaaatgt tttttgagca
     2821 aacgcgcact ttcttcagga tcggtatgcg caccacccaa tggttctttt acaataccat
     2881 cgataatttt attgcgataa ttatctgctg cagtaagttt caatgcttcg gcagcaactt
     2941 ctttataatc ccaactgcgc cataaaatgc tcgaacatga ttcaggagaa att
//
LOCUS       DAOQHY010000286         2945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_22658_length_2945_cov_3.897232, whole genome shotgun sequence.
ACCESSION   DAOQHY010000286 DAOQHY010000000
VERSION     DAOQHY010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22658_length_2945_cov_3.897232"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            427..1140
                     /locus_tag="PLL28_14615"
     CDS             427..1140
                     /locus_tag="PLL28_14615"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNF70612.1"
                     /translation="MTKLSVNINKVATIRNARGGSVPDVVQFAINCERFGADGITVHP
                     RPDERHITYKDVLALAPVVTTEFNIEGYPDARYMDIIRTTKPAQATLVPDPPNVITSN
                     TGWDVVQHMLMLRDIIAEIKSMGIRVSIFLNPDINQVHAAKESGTDRIEFYTGPYADH
                     FSTDKINAIQTYISASKTAHAIGLGINAGHDLNLQNLKFFKNSLVHLDEVSIGHAIIS
                     DALYFGIENTIQLYKRELL"
     gene            complement(1152..2189)
                     /gene="porQ"
                     /locus_tag="PLL28_14620"
     CDS             complement(1152..2189)
                     /gene="porQ"
                     /locus_tag="PLL28_14620"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HNF70613.1"
                     /translation="MKRILFISIFFSAISSVYGQFGGSYTYAYLQLPPSARISGLGGL
                     NISTYDNDIAMAWQNPALLNEGMDGDISANHAFMPSGISHGGVMYGKSLKNWHATMSA
                     GIQYRTYGTFQLTEANGDVVGSFTGSEYALHWGIGYGTGKLRYGANVQMLYSRLESYS
                     SFGMAADLAAAWVDTAKLMTVGLVMRNIGTQFKPYISDQYEELPFGIDLGVSKRLKHL
                     PLRVSFSLHDLQTFDIRYDDPNAIQDVNIFSTDTIVEEKNYTVDKIAQHLNLGGEFYF
                     GKNLMLRLGYDHMTRSEMAIETARGLTGFSMGVGMRIKKYSIDYGHEFYSQAGGSHHI
                     TIALNLNELRR"
     gene            complement(2275..>2945)
                     /locus_tag="PLL28_14625"
     CDS             complement(2275..>2945)
                     /locus_tag="PLL28_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008629106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HNF70614.1"
                     /translation="EAYAKTLQPADIIKILGPEKFDKESIFDNMPAGVVVGLAWTPVG
                     GDVLFIETSLSKGKGTLQMTGNLGDVMKESASTALAFIRANADKLGIAGEAFEQSAIH
                     IHVPEGAIPKDGPSAGITMLTALTSAFTGRRVKPFFAMTGEITLRGKVLPVGGIKEKV
                     LAARRAGVKNIIMCANNKKDVAEINPTFIKGVKFHYVESMQEVLDLALEPLKPAKAAK
                     VKRK"
BASE COUNT          815 a          712 c          566 g          852 t
ORIGIN      
        1 gtgagttttt ttcgagataa ggttgtttgt ggtgtgtggt tgtgatgtgc gattgtggtt
       61 tgtgtttttt cacgcggagg gagcggggga ggcgggggtc gcggttcgta tttaaacttc
      121 aattcttcaa accttcgaat cttcaactct cccaaccttc gaatcttcaa ttcttcaaac
      181 cttcgaacct tcgaatcttc aattctttaa accttcgaac cttcgaatct tcaattcttc
      241 aaaccttcga atcttcaatt cttctacctt cgaatcttca attcttctac cttcgaatct
      301 tcaatccttc gaatcttcga atcttcaact cttcaaacct tcgaatcttc aaacctttaa
      361 cccccaaccc tcaaccccca acatccctct cctcaattgc ttaactttgt aattcaaaca
      421 cagtcaatga ccaaattatc ggtaaacatc aacaaggttg caactattcg taatgcacga
      481 gggggaagtg tgcccgatgt agtgcagttt gctattaatt gtgagcggtt tggagccgat
      541 ggtatcaccg tgcatccgcg tcccgatgag cgtcatataa cctataaaga tgtactggca
      601 ctggcacctg ttgtcacaac cgagtttaat attgagggct atcccgatgc gcgatacatg
      661 gatatcatcc gaacaacaaa acccgcgcaa gctacccttg ttcccgatcc gccaaatgta
      721 attacttcta acaccggttg ggacgtagtg caacatatgc tgatgctgcg tgatattatc
      781 gctgaaataa aatcaatggg tatccgtgtt tccatattcc tgaaccctga tattaatcag
      841 gtacatgccg ccaaggaatc aggaaccgat agaattgaat tctatacggg tccctatgcc
      901 gatcatttct ctacagataa aataaacgcc attcaaacat atatcagcgc atcaaaaaca
      961 gcacatgcaa tcggattagg cattaatgcc ggtcatgact tgaatttgca aaatctgaaa
     1021 ttcttcaaaa acagtctcgt gcatctcgat gaggtttcca tcggtcatgc catcatttct
     1081 gatgccttgt atttcggcat tgaaaatacc atacaattat ataagagaga gttgctgtaa
     1141 aataccacct gtcagcgtcg cagctcattc aaattaagcg caatcgtgat atggtgcgag
     1201 ccaccggcct gggaataaaa ttcatggccg taatcaatac tgtatttttt tatccgcata
     1261 ccaacaccca tcgaaaatcc ggtgaggcca cgtgcagttt ctattgccat ttcactgcga
     1321 gtcatatggt catagccaag gcgtaacatc aggttttttc caaaataaaa ttcaccaccc
     1381 aaattcaagt gctgcgcaat tttatcaaca gtataatttt tttcttcgac gatagtatct
     1441 gtagaaaaaa tattgacatc ctgaatggca ttcggatcat cgtatctgat atcaaaagtt
     1501 tgcaaatcgt gtaaactgaa actcacacgc aagggaagat gcttcaatcg ttttgaaacg
     1561 ccgagatcta ttccaaatgg taattcttcg tattgatcgc tgatatatgg tttgaactgc
     1621 gtgccgatat tgcgcatcac taatccgaca gtcatcaatt ttgcagtatc tacccacgct
     1681 gcggcaaggt cggcagccat accaaacgag gaatagcttt ccaggcgact atacaacatt
     1741 tgcacatttg cgccatagcg taatttacct gtgccatagc ctattcccca atgcagggca
     1801 tattctgagc ctgtgaaact tcccaccaca tcgccgttcg cttcagttaa ttgaaaggtg
     1861 ccataggtgc gatattgaat acctgcactc atggtggcat gccaattttt taaagatttg
     1921 ccatacatca caccaccatg cgatattcct gaaggcataa aagcatgatt ggccgaaata
     1981 tcaccatcca tcccctcatt taatagtgca ggattctgcc atgccatggc aatgtcatta
     2041 tcgtaggtag aaatgtttaa gccacccaaa ccactaatcc tcgccgatgg tggtaattgc
     2101 aggtaggcat aggtataact gcccccaaac tggccgtaaa cagacgatat cgcgctaaaa
     2161 aatatgctga tgaaaagtat ccgcttcatg ttcgactgcg tgcttaacga aaagtcccgc
     2221 aaaaaatttt acgggactaa gttaatgaga tttatgtgtt tgagaagtct gacttcattt
     2281 gcgcttaacc ttcgcagcct ttgcaggttt aagcggctcg agtgcgagat ccaatacttc
     2341 ctgcatgctt tccacatagt ggaacttcac gcctttaata aaggtgggat tgatttccgc
     2401 aacatccttc ttattgttgg cacacataat gatgtttttc acgccggctc ttcttgcggc
     2461 aagcactttt tctttaatac cgccaacagg caataccttt ccgcgcaaag ttatttcacc
     2521 tgtcatggca aaaaatggtt tcacgcgacg gcctgtaaat gctgatgtca atgccgtaag
     2581 catggtaatc ccggcactcg gaccatcttt cgggatggca ccttcaggta cgtgaatgtg
     2641 aatggcactc tgttcaaatg cttcaccggc gatacccaac ttgtcggcat tagcgcgaat
     2701 aaatgccagc gctgtagaag cagattcttt catcacatca cccaaatttc ctgtcatctg
     2761 caaagtgccc ttgcccttgc taagcgatgt ttcgataaat aatacatcgc ctcctacagg
     2821 cgtccatgca agtccgacaa ccacaccggc aggcatatta tcgaagatac tttccttatc
     2881 gaatttttca ggacctaaaa ttttaatgat atctgcaggt tgcaatgttt ttgcatatgc
     2941 ttcat
//
LOCUS       DAOQHY010000287         2906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_23204_length_2906_cov_5.089442, whole genome shotgun sequence.
ACCESSION   DAOQHY010000287 DAOQHY010000000
VERSION     DAOQHY010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23204_length_2906_cov_5.089442"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PLL28_14630"
     CDS             complement(<1..583)
                     /locus_tag="PLL28_14630"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HNF70615.1"
                     /translation="MQEAFIQFIWRMQLFTASTLTTTDGEQISVINRGTLNTDSGPDF
                     LHARIRIGDTLWVGNVEIHLRSSGWLSHKHHVDRAYDNVILHVVYEHDMPQSDIPTLE
                     LKNIIDNSLIDRYELMMQTASWIPCAKTIAHVPPVIIRQHLIRMLAERLEEKALRFEQ
                     RLMLNSNDWEETCYQLIARSFGTNVNADPFERLA"
     gene            complement(770..1585)
                     /gene="pyrF"
                     /locus_tag="PLL28_14635"
     CDS             complement(770..1585)
                     /gene="pyrF"
                     /locus_tag="PLL28_14635"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNF70616.1"
                     /translation="MDRTQLIHQIKTKRSFLCVGLDTDLEKIPVHLLQESDPIFAFNK
                     AIIDATRAHCVAYKPNVAFYEAYGSKGWESLEKTIQYIGKEHFIIADAKRGDIGNTSA
                     RYAHAFFGAMSCDAITVAPYMGEDSVTPFFSYPDKWVILLALTSNKGSQDFQLIADGD
                     GALYEHVIRKAATWGNTGNLMYVVGATHPEYFSEIRKLIPDHFLLVPGVGAQGGDLHA
                     VCAHGMNTDCGLLINSTRDIIYASSGHDFAQAAAQRAMEISMQMNLLLQAKGI"
     gene            complement(1679..1921)
                     /locus_tag="PLL28_14640"
     CDS             complement(1679..1921)
                     /locus_tag="PLL28_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNF70617.1"
                     /translation="MNGNEALIQQVEASLDSIRPYLNQDGGDIKVVDITDDMVVKVKL
                     LGACESCPMSFMTMKAGVEATLKKTVPEIKAIETVD"
     gene            complement(1955..>2906)
                     /locus_tag="PLL28_14645"
     CDS             complement(1955..>2906)
                     /locus_tag="PLL28_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNF70618.1"
                     /translation="PMKEEIRRACVNAVKLLVSKEAEVDVNMSARVSSIRGNQEQLLP
                     GVKNIIAVCSGKGGVGKSTVSVNLALGLAKTGAKVGIMDADIYGPSIPVMMGLKDAQP
                     RVEEVNGKPMMIPLEAHGIKALSIGFLVDETQAVVWRGPMVSSALRQFVTDCIWGELD
                     YLIIDLPPGTGDIHLTLVQTVPVTGAVIVTTPQEVALADARKAVGMFRLPNINVPVIG
                     IVENMAWFTPEELPENKYFIFGTGGGTQLAKEYDVPLLAHIPLVQGIREGGDSGKPAI
                     LRNDIPVLTEAFDTLINNTARYISIRNEEKTATKIVEITR"
BASE COUNT          834 a          663 c          592 g          817 t
ORIGIN      
        1 ttgcaagcct ttcaaatgga tctgcattta catttgtgcc aaaagaacgt gctatcaatt
       61 gataacatgt ttcttcccaa tcattgctgt ttaacattaa tcgctgttca aatcgcaaag
      121 ctttttcctc cagcctctct gccaacatac gaattaaatg ctgtctgata ataacaggtg
      181 gaacatgtgc aatagttttt gcgcaaggta tccatgaagc tgtctgcatc atcaattcat
      241 acctatcaat aagtgaatta tcaatgatat tttttaattc caaagtagga atgtcactct
      301 gtggcatatc atgctcgtac acaacatgca aaatcacatt gtcataagct cggtcgacat
      361 gatgtttatg cgaaagccat ccggaactgc gcaaatgaat ttctacattc ccaacccaca
      421 aggtatcgcc tatgcgtatg cgcgcatgta agaaatcagg accactatct gtattaagtg
      481 ttcctctatt tatgactgaa atttgctcgc cgtcagtggt ggtaagggtg gaagcggtaa
      541 ataactgcat cctccagatg aactggataa acgcttcttg cataatgttg aatttttaag
      601 ggtaaacaaa aggataaaca tgaaacgaaa atacgaaaaa taggggaaag gagagaggag
      661 aaaggagaga ggaaaaagga gagaggaaag aggagagagg aaattgttat attgaaaaag
      721 aattctgcgc ctctgcgtga aactaaaatt ggaatcttga taaaataaat taaatccctt
      781 tagcctgcaa taacaaattc atttgcatgc taatttccat ggcacgttgt gctgccgctt
      841 gtgcgaaatc gtgtcctgaa gatgcgtaaa taatatcgcg agtggaatta atcagcaatc
      901 cgcaatctgt attcatgcca tgtgcgcaaa cagcatgcag gtcgccacct tgtgcaccta
      961 cgccgggcac gagtaaaaaa tgatcaggta ttaatttcct gatttctgaa aaatattcag
     1021 gatgtgtggc accaactaca tacattaaat ttccggtatt cccccaagtg gcagccttgc
     1081 gaatcacatg ttcatacaga gcaccatcgc catctgcaat taattggaaa tcttgcgaac
     1141 ctttattgct cgttaatgca agtaatatca cccatttgtc cgggtaggaa aaaaatggcg
     1201 taacactgtc ttctcccatg tatggggcta ccgtaattgc atcgcacgac atagcaccga
     1261 aaaatgcatg tgcatatcgc gcactggtat tcccaatatc gccccgcttg gcatctgcaa
     1321 taataaaatg ttctttgccg atatattgaa ttgttttctc caaactttcc cagcctttcg
     1381 agccatacgc ttcataaaat gccacattgg gtttgtacgc tacacaatgt gctcgcgtgg
     1441 catcaataat cgctttatta aacgcaaaaa tcggatcgct ttcttgcagt aaatgaacag
     1501 ggatcttttc gagatcggta tcgagtccga cacataaaaa actgcgcttc gttttaatct
     1561 gatgaatgag ttgcgtgcga tccatgaggc aaaattactc aattagagga aggccatgaa
     1621 aggagagagg aaaaaggaaa aaggaaaaag gagagaggag agaggagagg ggaatgtttc
     1681 agtctacggt ttctatggct ttgatttcgg ggactgtttt tttgagggtt gcttctacac
     1741 ccgctttcat agtcataaaa ctcattgggc aactttcaca ggcgccgagg agtttcacct
     1801 taacaaccat atcgtcggtg atatctacca ccttgatatc gccaccatcc tgattgagat
     1861 aggggcgtat ggagtcaaga gatgcctcta cttgttgaat caatgcctca ttaccgttca
     1921 tagtgcaaaa ttagtcatta ccaagcgcat tttcttatcg cgtaatctct acaattttag
     1981 ttgccgtttt ttcttcgttg cgaattgaaa tatagcgtgc tgtgttatta atcaatgtat
     2041 caaaagcttc tgttaacaca ggtatatcat tgcgtaaaat tgcaggtttg ccgctgtccc
     2101 caccttctct aatcccttga acaagaggaa tatgtgctaa taatggcaca tcatactctt
     2161 tagccaactg tgtgccgccg cctgtgccga agatgaaata tttattttcc gggagttctt
     2221 caggggtaaa ccaagccata ttctccacta tacctatcac cggaacatta atatttggta
     2281 atcggaacat acctactgct ttgcgcgcat ccgccagtgc cacttcctga ggtgtggtga
     2341 caatcacagc ccctgtaacg ggcactgttt gcaccaacgt aagatgtata tctcccgttc
     2401 ccggaggcag atctatgatc agatagtcca attcacccca aatacaatcg gtaacaaatt
     2461 gtcgcaacgc actgctaacc attggtcctc gccatactac cgcctgcgtt tcatcaacca
     2521 gaaagcctat cgataaggct ttgataccat gtgcttccag cggaatcatc atcggtttgc
     2581 cattcacctc ttcaactcgc ggttgggcat cttttagtcc catcatgacc ggaattgaag
     2641 gtccatagat atcggcgtcc atgattccca ccttggctcc tgtttttgcc agtccgagtg
     2701 ccagattcac cgaaacagta gattttccca cacccccctt gcctgagcat acggcaataa
     2761 tattttttac accgggtaag agttgttcct gatttcctcg gatagacgac accctcgcac
     2821 tcatgttaac gtcaacctct gcctccttgc ttaccagcag cttaacagca ttgacacatg
     2881 ccctgcgaat ctcctccttc atgggg
//
LOCUS       DAOQHY010000288         2889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_23434_length_2889_cov_5.926958, whole genome shotgun sequence.
ACCESSION   DAOQHY010000288 DAOQHY010000000
VERSION     DAOQHY010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23434_length_2889_cov_5.926958"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1851)
                     /locus_tag="PLL28_14650"
     CDS             complement(<1..1851)
                     /locus_tag="PLL28_14650"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70619.1"
                     /translation="MHLLRKYHFLIAALSCYLPIQLQAQEILLPLKNNTALMQREVVA
                     GSRSIVENRDTLCMPFMDDFSAAQIFVDGAFTECGDSIIQTSSSIYPSSIFWVDSNAF
                     VNRTYPSLPPTYGVATLDGLNKYGQPYNETSTLGPADMLTSKPIFVGGAITDSVYLSF
                     YYQPGGFGEYPNLNDSLLLDFKAEDGTWSNVWFTVNTLGDDPQSFKLVMIPVADAYHY
                     DGFQFRFRNWAGINGNNDHWHLDYVYLDDDRTYNDTLFRDVAIIYQPEPYLRRYRQMP
                     WNQFKDHQAEEISATHGVFMYNNFNTIVNTSHAYSVVEKYSGTEVMPLSAPVSVNLDP
                     FTIAFDSYPNFEIPEATPGYNDDSLSITFKYQLEPSGDINHRNDTLLHDQSFYNYFAY
                     DDGSAELAYQLIGLGSKLAMRFYANEPDTIKEIYIHWAYLDGSNSNKFFSLLLWDNID
                     TTGATADENIIYQNDFLTPKYIDSINGFYVYKLVDFLGNPAPVVVDGYFYVGWLQSQE
                     ASLDIGFDVNSDAKENVFYNVGGTWQQSALSGAIMLRPQLGGNYSMYAPVENESPGTS
                     SLMVYPNPATDILNINTATEEMQSIEIFDVDGRRVLVSDITSGTINIADLS"
     gene            complement(1867..2706)
                     /locus_tag="PLL28_14655"
     CDS             complement(1867..2706)
                     /locus_tag="PLL28_14655"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNF70620.1"
                     /translation="MSKKKQSSPESPGFLRFLMSKVFWLNVFGALVVAILLMWGTLFF
                     IQQYSRHGESQTVPNLIGKTTSEALNELDNLDLEYAVMDSTFDPDERPLTILNQDPLP
                     DSKVKSGRTIYVTVNMKQAPKTEIPRFEVGTSFISVREILESHGLKVGEIIYKPFEYR
                     DVYLDMRVHGEKRSLKPGTMIAKGTKIDVVLGNGLGDTKVIIPDLTGLTYIEAANLIQ
                     LKELSLGTVIAAGAISDSADAMVYKQFPTPGIDNAVNLGSMIDIWITDDPALLTPSSN
                     EDQ"
BASE COUNT          836 a          613 c          606 g          834 t
ORIGIN      
        1 cgataaatca gcaatattta ttgttccgga agtgatgtcg ctcaccaaaa ctctgcgtcc
       61 atctacatcg aaaatttcta ttgattgcat ttcttcagtg gcagtgttga tattcaatat
      121 atcagttgcc ggattcggat ataccattaa tgatgatgta ccgggcgatt cattttctac
      181 cggcgcatac atcgaataat tgccacctaa ttgtggtcgc aacataatag caccgctaag
      241 tgccgattgc tgccaggtgc cgccaacatt gtagaatacg ttttctttag catcgctgtt
      301 tacatcaaat ccaatatcca atgatgcttc ctgcgattgc aaccatccta cataaaaata
      361 tccatctaca acaaccgggg ccggattacc aagaaaatct acgagcttat acacgtaaaa
      421 tccgttgatg ctatcgatgt attttggtgt aaggaaatcg ttttggtaaa taatattttc
      481 gtcggcagtt gctcctgtag tatcaatatt atcccacaat aggaggctaa aaaatttatt
      541 gctattgctg ccatcaagat atgcccagtg aatataaatt tccttgatcg tatccggctc
      601 gtttgcatag aatcgcatgg caagttttga gcctaatcca attaattggt atgcgagctc
      661 agcagaaccg tcatcgtagg cgaagtaatt gtaaaacgac tgatcatgca acaaggtatc
      721 attgcggtga ttgatatcgc ctgaaggttc cagctgatat ttgaatgtga ttgacaaact
      781 atcatcatta taaccaggcg tagcctcagg aatttcaaaa ttcggatagc tatcaaaagc
      841 tatcgtaaag ggatccaggt taaccgatac aggagctgaa agcggcatta cctctgtgcc
      901 tgaatatttt tctacaacac tgtatgcatg agaagtattt acaatcgtgt tgaaattatt
      961 atacatgaac acgccatgtg ttgcagaaat ctcttctgcc tgatgatctt taaattgatt
     1021 ccatggcatc tggcggtatc tgcgcaaata aggctccggt tggtatatga tagccacatc
     1081 gcgaaacaag gtatcattat aggtgcgatc atcatcgaga tatacataat ccaaatgcca
     1141 atgatcatta ttgccattta tacctgccca attgcggaag cggaattgaa atccatcgta
     1201 atgatacgca tcagcaacag gaatcatcac caatttaaat gattgcggat catcgcctaa
     1261 agtatttacg gtaaaccaca cattcgacca tgtgccgtct tcagctttaa aatcgagtaa
     1321 tagagaatcg ttgagatttg gatattcgcc aaatccgccg ggttgataat aaaaactcag
     1381 ataaactgaa tccgtaatcg caccaccaac aaaaattggt tttgatgtga gcatatctgc
     1441 cggtccgagc gttgaggttt cgttatacgg ctgaccatat ttatttaatc catcgagtgt
     1501 ggcaacacca taggttggtg gtaaggatgg atatgttcta tttacgaatg cattgctatc
     1561 cacccaaaaa attgatgaag gataaatgga cgatgaagtt tgtataatac tatctccaca
     1621 ctccgtaaag gcaccatcaa caaatatctg agctgcgcta aaatcatcca taaacggcat
     1681 gcagagggta tccctgtttt ctacgatact gcgacttcct gcaacaactt ccctttgcat
     1741 caaagcggta ttatttttca agggaaggag aatctcctgt gcctgcaatt gtatcggaag
     1801 atagcaagag agtgcggcga tgaggaaatg atattttctt agcaaatgca tgatttaata
     1861 atagaattat tggtcttcgt ttgaggatgg cgtgagtaat gcaggatcat cggttatcca
     1921 gatatcgatc attgatccga ggttaacggc attatcgatt cccggcgttg gaaattgttt
     1981 gtacaccatg gcatcagcag agtcgctaat ggcacctgct gcaataactg ttcccagaga
     2041 aagctctttc agctggatca aattagctgc ttcgatatag gtaagtcctg taagatcggg
     2101 tatgataact tttgtatcac caagaccatt tcctaacacg acatcaattt tagttccttt
     2161 tgcaatcata gtaccgggct tcaatgagcg tttttcgcca tgtacgcgca tatcgagata
     2221 aacatcgcgg tattcaaagg gtttatatat gatctcgcca actttcaaac catgtgattc
     2281 taaaatctcg cgcacgctga taaagcttgt tcccacctca aaacgaggaa tttccgtttt
     2341 tggtgcctgc ttcatgttaa cggtaacata aattgttctt ccactcttca cttttgagtc
     2401 gggaagcggg tcctgattaa ggatggtaag cgggcgttcg tcaggatcga aggtagaatc
     2461 catcaccgca tactccaggt cgaggttatc tagttcattc aatgcttctg aggtagtttt
     2521 tcctataagg ttcggaactg tctggctttc gccgtggcgt gagtattgct gaataaagaa
     2581 cagagttccc cacataagca aaatggccac cacgagtgct ccgaacacat tgagccagaa
     2641 cactttactc attaaaaagc gcaggaagcc cgggctttcg ggggaagatt gttttttctt
     2701 actcatgcgt agcgcgcaaa gttacgtaaa atggaggctt caatggtcgc aggaagcacc
     2761 atctaacagc ccggtaaaga attcgtttaa ggtcatgccg gcagttttcg ccattttcgg
     2821 cactatacta gcctcgctga gacccggaac agtgttcact tccagaaaat gtggcactcc
     2881 gtttgcaat
//
LOCUS       DAOQHY010000289         2815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_24434_length_2815_cov_5.961232, whole genome shotgun sequence.
ACCESSION   DAOQHY010000289 DAOQHY010000000
VERSION     DAOQHY010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24434_length_2815_cov_5.961232"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..2735)
                     /locus_tag="PLL28_14660"
     CDS             complement(129..2735)
                     /locus_tag="PLL28_14660"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF70621.1"
                     /translation="MIKKLYVLAIGLLMAVGLQAQTVIKGNISAQDGGALPSATVMEK
                     GTNNGTVADLDGNFSITLIKTPATLVFTFVGYTTQEIEVSAQASLRVVMESGSYSLSG
                     VEVVGTRSLNRSATESMSPIDIIDVTAVTSANGQLDLNQLLQYTAPSFNSNRQSGSDG
                     ADHIDPATLRGLGPDQTLVLVNGKRYHQSSLINIFGTRGRGNTGTDLNTIPAAAIERI
                     EILRDGASAQYGSDAIAGVINIVLKDNVNEFTGNLNTGAYKAEYNFNEGSLDGQNLNL
                     NGNYGFKIGDDGFFNVTADYNYRGHTNRAEFIGPDFPALTDYRNQFGDAAVTNFTSWF
                     NMRIGLSNGASFYAFGGRNSRLVDAYAYTRSITGDNAHPERVVLDIYPEGYFDPIIAS
                     VVADNSLSAGIRGDMRGWDVDFNNTIGNNNMHYYGRNTLNASLGAASPTEFDDGGFSL
                     MQNTTSLDFSRYYASCLKGMNVAFGAEYRIENYNIFAGEEGSWYNYGGMYITETDTVE
                     RPGGSQGFPGFQPGDVTDEYRSNIGAYIDLEADFTDAFAMSAALRYEDYSDFGNTVTG
                     KIAARLAMGKNYALRGSFSTGFRAPSLPQIYFSSTYTNVESGVIVDEVIADNKSTIAR
                     ELGIPALKEETSVNGTIGITAKPMPALNITLDAYYVTIADRIVLTDGFGTDDDVIGGV
                     LEDLNVGYARFFTNAVDTKTTGLDAIISYKIYMKENTLTFTYAGNFNKMEVTAIHTND
                     LLAGKEDNYFSDREEYFLLASAPPVKMHLNAEYATKKFNANLRFNYFGEISLINYSGN
                     LYTYTPKYTIDASVGYDLTGNLHVTVGGANLTDQYPDPTDPFETETGGAWDAVQMGFN
                     GMFLFAKLGFTF"
BASE COUNT          794 a          607 c          619 g          795 t
ORIGIN      
        1 aataagtttg ttaaactgaa aaaaaatgcg gggttcacgc agagccgcag agaccagagt
       61 gtttgggggg aaataaaaac ggctccgcaa ggggagccgt tttgttatca ttaggcaatg
      121 gacgtcgctt agaaggtaaa tccgagtttt gcgaacagga acataccgtt gaagcccatt
      181 tgaacagcat cccaggcgcc accggtctcg gtttcgaacg gatcggtagg atcaggatat
      241 tgatcggtaa ggttggcacc acccacagtt acgtgcagat tgccggtaag gtcgtaacct
      301 acgctggcgt cgatggtgta ttttggtgta taggtataca ggttgccgct gtagttgatg
      361 agtgatatct caccgaaata attaaatcgc agattagcat tgaatttttt agtggcatat
      421 tctgcattca ggtgcatctt caccggaggg gcactggcga gcaagaaata ttcttcgcga
      481 tcgctgaaat aattatcctc tttacctgca agcaaatcat tggtatggat ggcagttact
      541 tccatcttat tgaagttgcc ggcatatgtg aaggtgagtg tattttcctt catataaatt
      601 ttatagctga tgatcgcatc aagacctgtc gttttggtat ctaccgcgtt tgtaaagaaa
      661 cgggcataac ctacattcag atcttccaat actccgccaa ttacatcatc atcagtaccg
      721 aaaccatcgg tcaatacaat acgatcggca atggttacat aataggcatc taatgtaata
      781 ttcaatgcag gcattggttt tgcagtgata cctatcgtac cgttcacgct ggtctcctct
      841 ttcaatgcag ggatacctaa ttcgcgcgcg atagtgcttt tattatcagc aataacttca
      901 tcaacaataa cacctgattc tacgttggta tatgttgagc tgaaataaat ttgtggtaat
      961 gatggtgcac ggaagcctgt gctgaatgaa ccacggagtg cataattttt tcccatggcc
     1021 aaacgcgcag caatttttcc tgttacggta tttccgaagt cactgtaatc ttcataacgc
     1081 aatgcagcgc tcatggcaaa tgcatctgtg aagtctgctt caaggtcgat ataagcaccg
     1141 atattactgc gatattcatc tgttacatcg ccgggctgga aaccggggaa tccctgagaa
     1201 ccaccgggac gttcaacagt atctgtttcg gtgatataca taccaccata attataccat
     1261 gagccttctt caccggcaaa gatgttatag ttttcaatgc gatattcagc accgaatgcc
     1321 acgttcatac ctttgaggca agaggcataa taacggctga aatcgaggct ggtagtattt
     1381 tgcatcagac tgaaaccacc atcatcaaat tctgttggcg atgcagcacc caaagatgca
     1441 ttcaaggtat tgcgaccata ataatgcata ttattattac cgatggtatt attaaaatct
     1501 acatcccatc cacgcatatc accacgaata ccggccgata atgaattatc agcaactact
     1561 gatgcgataa taggatcaaa atatccttca ggataaatat ctaataccac gcgttcagga
     1621 tgtgcattat ctcctgtgat agaacgcgta taagcatatg catctacgag gcgactattt
     1681 cttccaccaa atgcataaaa cgaagcaccg ttgctaagac cgatgcgcat gttgaaccag
     1741 gaagtgaaat tggttaccgc tgcatcaccg aactgattgc gataatcagt aagtgcagga
     1801 aaatcaggtc caataaattc tgcgcggttg gtatgtccgc gataattata atctgcagtt
     1861 acattgaaaa atccatcatc gccaatttta aatccgtagt taccattcag atttaaattc
     1921 tgaccatcga gtgatccttc gttgaaatta tattcagcct tatatgcacc ggtatttaaa
     1981 tttcctgtga attcattcac attatctttc aatacgatat tgattacgcc ggcgatagca
     2041 tcagaaccat attgtgcaga agcaccatcg cgcaaaattt cgatgcgttc gattgcggct
     2101 gcaggaatag tatttaaatc agtacctgta ttaccacgac cacgcgttcc gaaaatatta
     2161 atcagtgatg attggtgata gcgttttccg tttaccaaaa cgagtgtctg gtccggacca
     2221 agaccgcgca atgttgcagg gtcgatatgg tcggcaccat cagaaccgga ttgacggttc
     2281 gagttgaatg acggtgctgt gtattgcaat aattggttga ggtcgagttg tccgtttgca
     2341 cttgttacag cagtcacgtc aataatatct attggcgaca tgctttcagt ggcggagcga
     2401 ttaaggctgc gtgtaccaac aacctctacc ccactgagac tgtaagagcc ggattccatc
     2461 actacacgaa gtgatgcctg tgccgatact tcaatttcct gcgtagtata gcccacaaaa
     2521 gtgaacacca gcgttgcagg tgtcttaatt aaggtgatgg agaagtttcc atcgagatca
     2581 gccacagtcc cgttattggt tcctttttcc atcactgttg ccgaaggaag tgctcctccg
     2641 tcttgtgcgg aaatattccc cttaatgaca gtttgtgcct gtaatcctac agccatcagc
     2701 aagccgatgg ccaatacgta aagctttttg atcataaaat tgggttttgt gaagctaatc
     2761 taaggttttg tgatatagct gtaaaaacga tttgcacatg gcaggtgtgg agcgt
//
LOCUS       DAOQHY010000290         2790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_24786_length_2790_cov_5.396709, whole genome shotgun sequence.
ACCESSION   DAOQHY010000290 DAOQHY010000000
VERSION     DAOQHY010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24786_length_2790_cov_5.396709"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="PLL28_14665"
     CDS             <1..925
                     /locus_tag="PLL28_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNF70622.1"
                     /translation="LLLGLAIGDALGVPVEFLSRKQVRANPVTDMSGWGTHDQPPGTW
                     SDDSSLAFCLAEALVEGYNLHAIAQKFIRWKYQAYWTARNEVFDIGITTSNAIEQLAR
                     AVPPGDAGGKSESSNGNGSLMRIVPLVFYLKDKPIADRFAITRNVSALTHGHIRSVIA
                     CFYFVEYLRKILEGHRPLSAYHALQIEMPDFLNSMQIDAVEINHFNRLLQGEIYKLKE
                     EEIYSSGYVVHTLEASIWCLLTTENFVDAVLKAVNLGDDTDTTGAVTGAIAGLWYGLD
                     DIPSTWLNKLARKADIKKLAKALAKKQFNAA"
     gene            1042..1485
                     /locus_tag="PLL28_14670"
     CDS             1042..1485
                     /locus_tag="PLL28_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70623.1"
                     /translation="MLDELSKYKRNNHFFYTPTMDLGKVCNAPSDKSGVYIIYALKGG
                     RIELIYIGRSGKVLPDGSMFIRKAGLGGLKDRLVNGKQFGEPRRISWKKRLLAEKIEA
                     LDVYWFVTHNKTYVDCPRIVENNLLKRHMDIYGRLPRWNKEQLWS"
     gene            1550..2440
                     /locus_tag="PLL28_14675"
     CDS             1550..2440
                     /locus_tag="PLL28_14675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70624.1"
                     /translation="MPHQPKFNLTHYASFAAAVLAGMGNGKAEVVYTDIDPDIVLDAN
                     NGYYTIDIDGNGEMDFSFGLGTDYVNTYFTFYFYLQEIVANPFDENAIAGNSHFYPYP
                     AGLTYFFPYALELNDVISTDLLWQPNPPQFLAWKTWTDEYDGHCFNCYWNLYQEPDVL
                     DHFLGLRLMDADSNYRYGWLRCDVKEDGSILVLKDFAYETEADYKILAGSKTTFEEIK
                     PGTIVGEIYASGNVIHLVITRRPTDEYTVNVFNVSGQNVYTASNNSPFMEIELDVPKG
                     TYLCVVTSGAITFSKLVYVD"
BASE COUNT          816 a          565 c          619 g          790 t
ORIGIN      
        1 cctcctcctc ggcctcgcaa taggtgatgc acttggtgtg ccggtggagt ttttgtcgag
       61 gaagcaggta agggcaaatc ccgtaacaga tatgtcgggg tggggaacac atgatcagcc
      121 gccggggaca tggtctgatg atagcagtct ggctttctgc ttagcagaag cgctggtgga
      181 gggttataac ctgcatgcta ttgcacaaaa attcattcgc tggaagtatc aggcatattg
      241 gactgcccgc aatgaagttt ttgatatcgg cataaccaca agtaatgcca ttgagcaatt
      301 ggccagagct gtgcctcctg gagacgcagg aggaaaaagc gagtcgtcaa acggcaatgg
      361 atcgctcatg cgtatagtac cacttgtgtt ttatctgaaa gataaaccta ttgcagaccg
      421 ctttgcaatc acgcgcaatg tatctgcact tacacatgga catatacggt cggtgatagc
      481 ttgtttttat tttgttgaat atttgcggaa aatattggaa ggtcatagac cactttccgc
      541 atatcatgca ttgcagattg aaatgccgga ctttctgaat agtatgcaaa ttgatgctgt
      601 tgaaataaat cattttaaca ggctcttaca aggagaaata tataagttaa aagaagagga
      661 gatttacagt tccggatatg tggtgcatac ccttgaagcc tccatttggt gcttgcttac
      721 taccgaaaat ttcgttgacg ccgtgttaaa agccgtcaac ctcggcgacg acacagacac
      781 taccggagca gttaccggtg caatagccgg actttggtat ggtcttgatg atattccttc
      841 aacatggtta aacaaattgg caaggaaagc agacataaaa aagcttgcaa aagcactggc
      901 taaaaagcag tttaatgctg cttaaagaat ggatttggtt tatcaaacca taaaaatttt
      961 caaattgcgt attatttatt gctgattgaa cacaggtatt aatttccgtg atgccaatgg
     1021 aataatgatt aattttatct catgctcgac gaactatcaa aatacaaacg caacaaccac
     1081 ttcttttata cgcctacaat ggacttgggc aaggtttgta atgcacctag cgataaaagc
     1141 ggagtatata tcatctatgc tttaaaaggc ggtcgcatag aacttattta tataggtcgc
     1201 tcaggtaagg tgttgcctga tggtagtatg ttcatcagaa aagcagggct tggtggactt
     1261 aaagacaggt tggtaaacgg caaacaattc ggtgaacctc ggaggatatc gtggaaaaaa
     1321 cgattgctcg ctgagaaaat tgaggctctg gatgtgtatt ggtttgtcac acacaataaa
     1381 acctatgtgg attgtccccg cattgtagaa aataatctac tcaaaaggca catggatatt
     1441 tatggccggc taccgagatg gaacaaggaa caattatggt cctagccgaa taaagatatt
     1501 cactatcaat tttccttaat ttgtcatccg tatgcctcaa acatccccca tgccccacca
     1561 accaaaattc aacctcaccc actacgcctc ctttgctgcg gcagtacttg ccgggatggg
     1621 taatggtaag gctgaagtag tctatactga tatcgacccg gatattgtat tggatgccaa
     1681 taatggatac tatactatcg atattgatgg aaatggagaa atggatttta gttttgggtt
     1741 gggtacggat tatgtaaata cctatttcac cttttatttt tatttgcagg aaattgttgc
     1801 caacccgttt gatgaaaatg caattgccgg caactcgcat ttttaccctt atcccgccgg
     1861 actcacgtat tttttccctt acgcgcttga attaaatgat gtcatatcca cggatttgtt
     1921 gtggcaaccc aatccgcctc agtttttagc atggaaaacc tggacggatg aatacgacgg
     1981 ccattgtttc aattgttatt ggaatctata tcaagaaccc gatgtgttag atcattttct
     2041 cggactaagg ttaatggacg cagacagcaa ttacagatat ggctggttaa ggtgtgatgt
     2101 gaaggaggat ggcagtattt tggttttgaa ggatttcgct tatgaaactg aagctgatta
     2161 taaaatttta gcgggttcta aaaccacctt cgaggaaata aaacccggga caattgtcgg
     2221 agaaatttat gcctccggaa atgttattca tcttgttatt acacgtcgcc ctacagatga
     2281 atacaccgtg aatgtcttta atgtctccgg acaaaatgta tatacggcat ccaacaattc
     2341 tccatttatg gaaattgaat tagatgttcc taaaggaacc tatttatgtg tggtgacatc
     2401 cggtgctatt acatttagta agttggtgta tgtcgattaa attccaacac gaacacaatc
     2461 attgtctgaa tcacggatta tttggattaa aggattacac ggattgcccg cttaagcaca
     2521 ttccacaaat caacccgcac ccgaacacaa ttagagtgtg caagtcccgc ttaggcggga
     2581 tgtcgctgcg ctccatgtgc aggtaaatgc aaaagcagaa cacatgaaca caaatcccat
     2641 gaactgcggg tattatactt caaattctcg tgaatcagcc tctattgcgt gcgtaaagca
     2701 tcgtcaaccc catcagctcc tctgccagct cattccgaag atcattaacg cccattctcc
     2761 agagccatac gttttcggtg gagccgggga
//
LOCUS       DAOQHY010000291         2789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_24806_length_2789_cov_5.341624, whole genome shotgun sequence.
ACCESSION   DAOQHY010000291 DAOQHY010000000
VERSION     DAOQHY010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24806_length_2789_cov_5.341624"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            249..677
                     /locus_tag="PLL28_14680"
     CDS             249..677
                     /locus_tag="PLL28_14680"
                     /inference="COORDINATES: protein motif:HMM:NF020157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HNF70625.1"
                     /translation="MEFKINMVAVLVTVVVNFILGFLWYTPLFGKAWGKEMGYPADMK
                     PDPKVLMKGMIFMVIGNFLFAYVLAHNIEAWSFVPDMDQTSQFSMALSSAFFLWLGFY
                     LPGELGATVWEKHSWKLFFINTVYHFLSLLVASVILTYWT"
     gene            complement(739..>2789)
                     /locus_tag="PLL28_14685"
     CDS             complement(739..>2789)
                     /locus_tag="PLL28_14685"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF70626.1"
                     /translation="TMPVTHTNIYASQLEQINLGRDMPFLLESVPSTVATSDAGNGVG
                     YSGLRIRGSDATRINITINGVPYNDAESQQTYWVDLPDFASSVEDIQIQRGIGITNNG
                     LSAFGASININTNNLNKETGAFVQSSVGSFGLRKFSGGYQSGLINEHWYIQGRASIMH
                     SDGYIDRAFADLGAWFMTGAYKGNKYTSIVNVFSGKERTYQAWGGVPAEVLDTNRTYN
                     PYTYENQTDNYRQTHVQWHQYFNVTNRDNLRLTLNYTKGSGYYEQLEEDQDFGDYGIE
                     PVLIGADTISNADLITQKWLDNDFYGAFLQYKHRHSNNWNTSTGATFYRYTGTHFGKV
                     IWNEFASTMGYDYTWYTNDAEKSDGSVYLQSDIKVGNILYLVDLQARYVGYQFLGYNV
                     EGLPVDQNVAIYSFNPKVGLSFFHGFKSKAYLFVGMSTKEPNRDDYTESTPLSRPVPE
                     KMIDVEIGDKLELDKFRISANYFMMYYIDQLVLTGEINDVGAYTRTNIPRSYRTGLEV
                     EYRYRIFPMLFLNGNVTYSINKALDYVAYIDNWDDGTQVPVAYASADLAFSPEWITYN
                     QLYGKIWDKNAASSTRYLLTATWTSKTVGQQYVDNTSDGLRSLDAYWLNDVGLNMMIT
                     AKRFKEMNISLQALNVFNKAYASNAWVYTYQYGGEIQQMMGYYPQAGINWQLGFSLKL
                     "
BASE COUNT          822 a          595 c          478 g          894 t
ORIGIN      
        1 aatgcgtttt tcaataatcc gaatttcaat accacaattc tttagaacta caagatttag
       61 caaacggatt aaattttgtt aatgtttaat ctgatgctca acaatttgta tctttagaga
      121 gcagatattt aaagttattt tgaagtatct gtcaggcaac atttgaaact tgtggagtct
      181 agaaaccacc ataataaacg gggtgtaacc gaaaagccaa atgagtaggc atcaaaactt
      241 ttttttctat ggaatttaaa attaacatgg tagccgtgtt ggttaccgta gtcgtgaact
      301 tcattttagg cttcctttgg tacacaccat tattcggaaa agcctgggga aaagaaatgg
      361 gctaccctgc cgacatgaag ccggacccaa aagtgctcat gaaaggtatg attttcatgg
      421 taatcggaaa cttccttttt gcctatgtgc tcgcgcataa tatcgaggca tggagttttg
      481 ttccggatat ggaccagaca agccaattct ctatggcttt aagttcagct tttttccttt
      541 ggcttggatt ttatttacca ggggaattgg gcgcaactgt atgggaaaaa cattcatgga
      601 aactgttttt catcaatacc gtttatcact tcctctcttt attggttgcc agtgtaattc
      661 ttacttactg gacttaaaaa taaagaaaac taaagcacct ttcatttcta aaaaagtgaa
      721 aggtgctttt ttattttttt ataatttcaa tgaaaatccc aattgccaat ttatcccggc
      781 ttgtgggtaa taacccatca tttgttgtat ttctcctcca tattgataag tatacaccca
      841 tgcattactt gcatatgctt tgttgaatac atttaatgcc tgtaaagaaa tattcatttc
      901 tttaaatcgc tttgccgtaa tcatcatatt caaacccaca tcattcaacc aatacgcatc
      961 cagacttcgc agtccatctg aagtattgtc tacgtattgc tgaccaacag ttttcgatgt
     1021 ccatgtagct gttaataaat atcgtgttga ggacgctgca tttttatccc aaatttttcc
     1081 atacaattga ttgtaagtta tccattcagg cgaaaatgcc agatcagcac ttgcatatgc
     1141 aacagggacc tgggtgccgt catcccaatt atcaatatag gcaacataat ctagagcctt
     1201 gttgatactg tatgtaacat tcccatttaa aaacaacatg ggaaaaatgc gatatcgata
     1261 ttcaacttcc agtcccgttc tgtagcttct cggaatattt gttctggtgt atgctcctac
     1321 atcattaatc tcacctgtca acacaagctg atcaatataa tacatcataa aataattagc
     1381 cgaaattctg aatttatcta gttccagttt atcacctatc tcaacatcaa tcattttttc
     1441 cggaacaggt ctgcttaaag gcgttgattc agtgtaatca tccctgtttg gctcttttgt
     1501 actcatgcca acaaacagat aagccttaga cttaaaccca tggaaaaatg aaagcccaac
     1561 cttaggatta aaagaataga tcgctacatt ctgatcaacg ggcaggcctt caacattata
     1621 tccaagaaac tgatagccca cgtatcgcgc ttgcaggtca actaaatata agatattccc
     1681 caccttgata tcactttgta aatacacact gccatcagac ttttcggcat cattggtata
     1741 ccacgtataa tcatatccca tagttgaagc aaactcattc caaatcactt ttccgaaatg
     1801 cgttccggta tatcggtaaa aagttgcccc tgtactggta ttccaattgt tgctatgtcg
     1861 atgtttgtat tgtaaaaacg ccccataaaa atcgttgtcc agccattttt gtgtaataag
     1921 atcggcattg gaaatggtgt ctgcaccaat caatacaggt tcaattccgt aatcgccaaa
     1981 atcctgatct tcttccaact gctcataata cccgctacct tttgtataat tcaatgtcag
     2041 gcgaagatta tctctgttcg ttacattaaa atactgatgc cattgaacat gcgtttgtct
     2101 ataattatct gtctgatttt cgtaggtata gggattataa gttctattgg tatctaatac
     2161 ttctgccggt acgccgcccc aggcttgata tgtgcgttcc tttcctgaaa atacattgac
     2221 aatagaagtg tatttatttc ctttataagc gcctgtcatg aaccatgcac caagatctgc
     2281 aaatgctctg tcgatatacc catcgctatg cataattgat gcacgacctt gaatatacca
     2341 gtgctcattg attaaaccac tttgatatcc cccggaaaat tttcgcaatc caaaagaacc
     2401 aaccgacgac tgcacaaatg cacctgtttc tttattcaaa ttatttgtat tgatattgat
     2461 tgacgcacca aatgcactca atccattatt agtgattccg ataccgcgct gaatttgaat
     2521 atcctctaca ctcgaagcaa aatccggcag atcaacccag tatgtttgtt gcgattcggc
     2581 atcgttgtag gggacgccat taatcgtgat attaatcctc gtagcatcac ttccgcgaat
     2641 gcgcaaacca ctgtatccta cgccgtttcc tgcatctgat gttgcaactg ttgatggcac
     2701 cgattccagt aaaaacggca tatcgcgacc aagatttatc tgttctagtt gcgaagcgta
     2761 gatattagta tgggtaaccg gcatagttg
//
LOCUS       DAOQHY010000292         2762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_25193_length_2762_cov_5.015515, whole genome shotgun sequence.
ACCESSION   DAOQHY010000292 DAOQHY010000000
VERSION     DAOQHY010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25193_length_2762_cov_5.015515"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..1476)
                     /locus_tag="PLL28_14690"
     CDS             complement(28..1476)
                     /locus_tag="PLL28_14690"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF006139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNF70627.1"
                     /translation="MSNNYELKLKEWNEKEMAAVEISTMATRLWFDKSVELIIFRRQL
                     VDARASEILNHHLYAQRFLNRPLTIEVTLLLTRALAKLDLAPSRIDMGRLGVEWLNEG
                     SNFADADAFVAGKMKDFIGKEKLVLKPKDVVLYGFGRIGRIAARVIINEAGKGEQLRL
                     RAIVTRSNSDEDILKRASLLRRDSIHGKFAGTIVEDLENKALIINGHTVHMIAADKPE
                     LIDYTAFGINDALVIDNTGIAREREKLSKHLEAKGVSQVLLTAPGKGDIPNIVHGINH
                     EKFDPSKEKIFSAASCTTNAVVPILYVMDKTFGIHHGHLETVHSYTNDQNLLDNYHKK
                     SRRGRSAPINMVITETGAASAAAKVLPELAGKFTGNAIRVPTPDVSLAIINLTLNKEV
                     TIDEVNNVLREAASFGTLVEQIDYSISNEIVSSDCIGNSHAVIVDSPATIVSPDKKSV
                     VLYCWYDNEFGYTKQVIRLSRYLAGVIRLRYY"
     gene            1760..2143
                     /locus_tag="PLL28_14695"
     CDS             1760..2143
                     /locus_tag="PLL28_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HNF70628.1"
                     /translation="MAKKKQYTLEYIIKSSPLVLYNCLTTATGLQQWFADLVDNDGNT
                     FFFTWKNDTETAELISTEEDDYIRFRWDWQDDDEYFEFRIDKTEITNDTILYITDFAE
                     DREVDAQKRLWDAQIQNLIKRIGAG"
     gene            2261..2449
                     /locus_tag="PLL28_14700"
     CDS             2261..2449
                     /locus_tag="PLL28_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70629.1"
                     /translation="MQLITRFPLQLLAKAIAIVCGVSTAIGARRWFLQTIIFFTINSI
                     NNKHKKLRFAKSFPLPFS"
BASE COUNT          810 a          649 c          551 g          752 t
ORIGIN      
        1 aatcttcaaa tctacctcac cttcaactca ataatacctc aacctaataa ccccggccaa
       61 ataacgcgac aagcgaatta cctgttttgt atatccaaac tcattatcat accagcaata
      121 cagcacaaca cttttcttat caggagacac gatggttgca ggtgaatcaa caatcaccgc
      181 gtgactgttt ccgatacaat cgctgctcac aatttcattg ctgatgctgt aatcaatttg
      241 ctctaccagt gtaccaaaag acgctgcttc acgtaaaaca ttgtttactt catcaatggt
      301 cacttcttta tttaaagtaa ggttgatgat cgcaagtgac acatcaggtg ttggaacgcg
      361 gatggcattg cctgtaaatt tccctgccag ttccggtaat accttcgcgg cagcacttgc
      421 agcacccgtt tctgtaatta ccatattgat tggcgcagaa cgtccgcgac gagatttttt
      481 atgatagtta tcgagcaggt tttggtcgtt tgtatatgaa tgcactgtct cgaggtgacc
      541 atgatgaata ccaaaagtct tgtccatcac atacaggata ggcacaacgg cattggtagt
      601 acagcttgct gcagagaata ttttttcttt tgaaggatca aatttctcgt ggttgatgcc
      661 atgcacgata ttaggaatat caccttttcc gggagctgtc agcaatacct gacttacccc
      721 tttcgcttcg aggtgtttgc tcagtttttc acgctcgcgg gcaataccgg tattatctat
      781 caccaatgca tcattgatac caaaagcagt ataatcaatc aactcaggct tatcggcagc
      841 aatcatgtgc actgtatgac cattgatgat gagtgcttta ttttcgaggt cttcaactat
      901 ggtgcccgca aatttgccat gaatagaatc gcggcgcagt aagcttgcac gtttcaggat
      961 atcctcatcg ctgttgctgc gtgtaacaat ggcacgtaaa cgcaactgtt caccctttcc
     1021 ggcttcgtta atgataacgc gggcggcaat acgaccaatt cggccaaaac cgtacaacac
     1081 aacatctttc ggcttaagca ccaatttctc tttgccgata aaatccttca ttttccctgc
     1141 cacaaaagca tctgcatccg caaaattgct gccttcattc agccattcca cacccaaacg
     1201 gcccatatca atgcgcgatg gtgccaaatc gagcttagca agcgctcggg tcaacaatag
     1261 cgtcacctcg atggtaagcg gacggttcag aaaacgctgt gcatacaggt ggtgattgag
     1321 gatctcgctg gcacgggcat ctaccaactg acggcgaaaa atgatcaact caacagactt
     1381 gtcgaaccat aaacgggtgg ccattgtgct gatttccaca gctgccattt ccttctcgtt
     1441 ccattctttg agtttcaatt catagttgtt gctcatatat cttgtgggtt ataaggttga
     1501 aaaattgaag tgcaaaggta aggaaaaagg ggcttacgag gggtgtcaaa acagccgata
     1561 tatcacgttt ttaggctttt tagcagctct aaaccacttg caatcacttg taaaacagca
     1621 aattagcagt aaatccctaa tccaccgcta aaatcagtca agatgttcac tcaatgtaga
     1681 taaaacataa aacaaaattt tgtatccttc gaaattatat tatacttgca ccgcaccaag
     1741 ttttaccggt aagcacaaaa tggctaaaaa gaaacaatac acgctcgaat atattataaa
     1801 gtccagccct ctggtactgt ataattgttt gactacggct accggcttgc agcagtggtt
     1861 tgccgatttg gtcgacaatg acggcaatac cttctttttc acctggaaaa atgatactga
     1921 aacggcagag ctcatttcta ctgaagaaga tgactatatc cgctttcgat gggattggca
     1981 agatgatgat gaatacttcg aatttcgcat cgataaaaca gagatcacca acgacactat
     2041 tctctacatc accgattttg ccgaagatcg tgaggtagat gcccaaaaaa ggttgtggga
     2101 tgcacagatt caaaatctga tcaaaagaat cggggccggt tgatcaaagc ctgacctacc
     2161 gcgccggttt ttacaggaaa cattttaccc gtattttata tttcaacaaa tcgcaggctg
     2221 aagtatttta ataatcagga gcactcaaat tccccactgc atgcaactga tcacccgctt
     2281 tccgttgcaa ctcctcgcca aggctatcgc cattgtctgc ggggtttcca ctgcaatcgg
     2341 ggctaggagg tggtttttac aaacaattat ttttttcacc attaattcga tcaacaacaa
     2401 gcataaaaaa cttcgcttcg caaaaagctt tccgctacct ttttcatgac aaaaaggtat
     2461 caccaaaagt caaggggtgt atgatcatat ggtcagataa aaagcacatg ctaaaataca
     2521 tcaaacacat tccacatagc cctcagaaaa acaaaaatct ctctcgccac gacagggcat
     2581 ttgcagatgc tgacaacagc cctaattacg cattcatttt ggaagtgaat gtttaagaat
     2641 aagttccatt aaatacaaaa tctgctccct ctctacgacg aaggagtgga gagggctggg
     2701 gtgaggccgg gccatgacgc aagcattagg gctgtgtcat gcagctgcag aagccctgag
     2761 ct
//
LOCUS       DAOQHY010000293         2718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_25936_length_2718_cov_6.231318, whole genome shotgun sequence.
ACCESSION   DAOQHY010000293 DAOQHY010000000
VERSION     DAOQHY010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25936_length_2718_cov_6.231318"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="PLL28_14705"
     CDS             <1..621
                     /locus_tag="PLL28_14705"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNF70630.1"
                     /translation="ISCVGVAGTIDNDLYGTDYTLGFDTAINTAIDAIDKIRDTAMSH
                     NRLFFVEVMGRDAGFIALSTGIAGGAEAIVIPETETNIDYLISTLERGWLRQKSSAIV
                     IVAEGDERGGAFEIAKLVKERFNHYDTRVSVLGHIQRGGNPTVRDRILGSRLGMAAVE
                     ALIEGRKNVMAGQVNDEVVFTPFANATKHHMEINKQMLQLVEILAM"
     gene            complement(764..1528)
                     /gene="rlmB"
                     /locus_tag="PLL28_14710"
     CDS             complement(764..1528)
                     /gene="rlmB"
                     /locus_tag="PLL28_14710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNF70631.1"
                     /translation="MREGEGNFKRKKFDNLVFGRQPVVELLMRESPVDKILLTKTAQG
                     DIIPQIKHLAGEKQVPVQYVPVEKLNQLTGGNHQGVIAFTAAVAFQKIDDILPFIIEK
                     GEVPLILILDGITDVRNFGAIARTAYAGGVHAIIIPAHHAAPVNSDAMKTSAGALAEI
                     PICREKNYELVLKYLKLNGLHIIGADAHADKFIYNSDLTVPTAIIMGSEDEGISAISR
                     KHLSETVKIPMQNSFDSYNVSVAAGMIIYEVMRQRA"
     gene            complement(1509..>2718)
                     /locus_tag="PLL28_14715"
     CDS             complement(1509..>2718)
                     /locus_tag="PLL28_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70632.1"
                     /translation="LGVDWYINQLRNATNDSKPIALTLGPEKILGDKRNQIVSNDKSK
                     FKNTTMDINDFLNFVGQDDPKYQIYSEGLEDYFSYMPTDKLKISIDNEAMKSQGIVPE
                     SLQGDMRKEISFKLTNKTLLKNDLMTLDIVANNINKRPICFAVSVTGESHLGMQKYLM
                     QRNMVYQLMPVEMENAKGVMDGFQKGIETDIMYDQLINQKDKFTYGGVEKGKPIYMDP
                     SAYGSILTAKYLNYLDLVKSLNDERAYLEAQARQMMKDTVNQEYTEVGQELMKESAAK
                     KEKMITVLDQMMVLFPDSSVPYDNNMVNVAQLYQQLGKPEKASEIINTMMPRTFSELE
                     YYYQMARNSTATAQMYQDDQMRSEQTVYLGIEILKKSDAKDQADTYDKQWEALRMKYG
                     IPDLNERRRR"
BASE COUNT          746 a          536 c          571 g          865 t
ORIGIN      
        1 atttcttgtg taggtgttgc cggtacgatt gataatgatt tgtatggcac cgattataca
       61 ctcggatttg acacagccat caatactgca attgacgcca tcgacaaaat aagagatact
      121 gccatgtcgc ataacaggtt gttttttgtt gaagtgatgg gacgtgatgc cggatttatt
      181 gccttgagta caggtattgc cggtggtgca gaggcaattg taattcctga aacggaaaca
      241 aatatcgatt atctcatttc tactttggag cgaggctggt tgcgacaaaa atcatcagcc
      301 attgttattg tagccgaagg tgatgagaga ggtggcgcat ttgagattgc taaattggtg
      361 aaggaaagat ttaatcatta cgatacacgt gtttctgtgt taggacatat ccagcgcgga
      421 ggcaatccta ctgtgcgcga ccgcattctc ggaagcagac ttggtatggc ggcagttgaa
      481 gctttgattg aaggaagaaa gaatgtgatg gcaggtcagg tgaatgatga agttgtgttt
      541 acgccatttg ctaatgctac aaagcaccat atggagatta acaagcagat gcttcagtta
      601 gttgagatat tggccatgta aaacggtctg gttttgtcct gatcttataa gatagtaatc
      661 gaatatatag cgaaatactt agagtatagg ctatgattac ggcacagaca tcagcattta
      721 tgtgttgatg cttaaaaaaa atgataatgc cgggagtatg attttacgcg cgttgacgca
      781 tcacttcata aatgatcata cctgctgcaa cagatacgtt ataagaatcg aaggaatttt
      841 gcatgggaat ttttactgtt tcgctgagat gcttgcgtga aattgcgctg ataccctcat
      901 cttcagaacc catgataatt gctgtaggaa ccgtgaggtc gctgttgtag ataaatttat
      961 cggcatgtgc atctgcaccg ataatatgca agccgtttag tttgagatac ttcaatacta
     1021 actcataatt cttttcgcga caaataggaa tttctgccag cgcgcctgca ctggttttca
     1081 tggcatcact attaacgggg gctgcatgat gtgcaggaat aatgatggca tgtacgcctc
     1141 cggcataagc cgttcgggca atagcaccaa aattgcgcac atcggtaatg ccatctaata
     1201 ttaaaatcag cggcacttca cctttttcaa tgataaacgg aagtatatca tcgatttttt
     1261 gaaaagcaac ggcggcagta aaggcaatta caccctgatg attgccaccg gtaagttgat
     1321 ttaatttttc aacaggaacg tactgcacgg gaacctgttt ttcgccggca agatgtttta
     1381 tctgcggtat aatatcgcct tgggcagttt tcgtcaataa aattttgtct accggcgatt
     1441 cacgcataag cagttcgaca accggttgtc tcccgaaaac cagattgtcg aacttcttgc
     1501 gtttaaaatt accttctcct tctctcattt aaatcgggta tgccatattt catgcgaaga
     1561 gcttcccact gtttatcgta agtgtcagct tgatcctttg catcagattt tttaagtatt
     1621 tctataccca ggtagactgt ttgctcagaa cgcatctgat catcctgata catttgagcc
     1681 gttgcagtgc tgttgcgagc catctgataa taatattcaa gttccgagaa tgtgcgcggc
     1741 atcatcgtgt taatgatttc agatgctttt tcaggtttac ctaattgctg ataaagctga
     1801 gcaacattca ccatattgtt atcataagga acactggaat cagggaataa taccatcatc
     1861 tggtcgagaa ccgtaatcat tttttctttc ttagcggcac tctccttcat caattcttgt
     1921 ccaacctcag tgtattcctg attaacggta tctttcatca tctgacgagc ctgcgcttca
     1981 aggtaagcgc gttcatcatt caacgatttt acgaggtcga gataattcaa atacttagca
     2041 gttaaaatac tgccgtaagc acttggatcc atataaatag gttttccttt ctcaacacca
     2101 ccgtaagtga atttatcttt ctggttgatc aattgatcat acatgatatc agtctcaatg
     2161 cctttttgga aaccatccat aacacctttg gcattttcca tttccaccgg cattaattga
     2221 tataccatat tgcgttgcat cagatatttt tgcatgccaa gatgtgattc gccggtaaca
     2281 ctcaccgcga aacaaatcgg acgcttgttg atattattgg caacaatgtc taatgtcatt
     2341 aagtcgtttt tcaacagcgt cttatttgtc agtttgaagc taatctcttt acgcatatcg
     2401 ccctgtaatg attccggaac gataccttga gatttcattg cttcattgtc gatggaaatt
     2461 tttaatttat cagtaggcat gtagctgaaa tagtcctcta agccttcact gtaaatttga
     2521 tatttaggat catcctgacc aacaaagttg aggaaatcat taatatccat tgttgtattc
     2581 ttgaacttgc tcttatcgtt ggaaacgatt tgatttcgtt tgtcgcctaa tattttttca
     2641 ggccctaatg ttaatgctat aggtttggaa tcgttcgttg cgttgcgtaa ttggttgata
     2701 taccagtcga caccaagc
//
LOCUS       DAOQHY010000294         2688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_26433_length_2688_cov_5.241170, whole genome shotgun sequence.
ACCESSION   DAOQHY010000294 DAOQHY010000000
VERSION     DAOQHY010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26433_length_2688_cov_5.241170"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PLL28_14720"
     CDS             <1..620
                     /locus_tag="PLL28_14720"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70633.1"
                     /translation="NEVLMYNFNLEVGDTLDFGDTSEVKIVTSIDSVLMNTKWHKRMH
                     ITPEYAPVENYVDLIEGIGSTFGLNQGVEFPFEESMRLLCFSNDDSLYINFYVDEIPI
                     SVDSAEICDFLPSNVSIDGLQQQLNFDVYPNPVGHIMHIDLHASLAGVNDILLFNSIG
                     ELSKHLWHIDSASLEINVSDLLPGMYVLVVRGINAAAISTTVIIQ"
     gene            complement(677..1291)
                     /locus_tag="PLL28_14725"
     CDS             complement(677..1291)
                     /locus_tag="PLL28_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70634.1"
                     /translation="MKLITTLLFCLGSLLAYSQDTIYLKVHFLYGSKPLKAYKESEPK
                     WFGGVLGGHVGIESDSNDILNFLPSGEFHLFSKQDERNSRFALHTEDNFYAMFGSDPD
                     SVKKTIIYIPISALQKQKFDSIANAYLNQTPYDYAFLGMRCGAAAYDVLGQLGILTEY
                     DHQKTYRKIFYPKKLRKPLLEKAEQNNWTIEKHAGSVTRRWEKD"
     gene            complement(1360..1851)
                     /locus_tag="PLL28_14730"
     CDS             complement(1360..1851)
                     /locus_tag="PLL28_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70635.1"
                     /translation="MKPSIVIIFLLISNISFGQLKLEDLQIDTTITGFHLAADFQGTI
                     VYTKNGPSDIQTQNPTAFAFTIMENLSAKKAKKQLYKLLSMSIKAGYTISDKIEKDTT
                     LNGNVCYYLSYKETNLQYNYQNFVFNAFVIKDKTAVIFTSGDLDNRRYYDKFKKTFYS
                     IKI"
     gene            complement(2049..2351)
                     /locus_tag="PLL28_14735"
     CDS             complement(2049..2351)
                     /locus_tag="PLL28_14735"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HNF70636.1"
                     /translation="MAFKGVPFSFKAVPWQYQGQGAWVFVDLPKDTANEIRSIFKNEE
                     QGWGRLQITAKIGNTEWKSAIWYDTKRKTYLLPLKAEIRNKEAVEIGVECAVRIWV"
     gene            complement(2375..>2688)
                     /locus_tag="PLL28_14740"
     CDS             complement(2375..>2688)
                     /locus_tag="PLL28_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70637.1"
                     /translation="GRNKQRSKTAEEIFKQDQRFEIRSAGVSASADKKLHTRDLAWAD
                     LVLVMESDQRDKIIEIFGRDIPKIIVLHIPDEYAYMDPELIAMLSDSINDILQSEFNI
                     T"
BASE COUNT          748 a          491 c          513 g          936 t
ORIGIN      
        1 caaatgaagt gttgatgtat aattttaatc tggaggtagg ggatacttta gatttcggcg
       61 acacttccga ggtgaaaatc gttacaagca ttgatagtgt ccttatgaat acaaagtggc
      121 acaaacgaat gcatattact ccggaatacg ctcccgtaga gaattatgtt gatttaattg
      181 aaggaattgg atctaccttc ggtctaaatc agggagttga atttcccttc gaagaaagta
      241 tgcggttgtt atgtttttct aatgatgatt cattgtatat aaatttttat gttgatgaaa
      301 ttcctataag tgtagattct gctgagattt gtgattttct gccttccaat gtttcaatag
      361 atgggttaca acagcagctg aattttgatg tatatcctaa tccggttgga catattatgc
      421 atattgattt gcatgcctca ttggcaggag taaacgacat actgttattt aatagcattg
      481 gtgaattatc caaacattta tggcatattg attccgcatc tcttgagata aatgtatcag
      541 atctgttacc cggaatgtat gttttggtag taaggggcat taatgcggcg gctattagta
      601 ctactgttat tattcaataa tgcgccttgt gtgttgttaa ccggaaattg atgactatgt
      661 ttgactgaaa taccaatcag tctttctccc atcttcgtgt aacagaacct gcatgctttt
      721 cgattgtcca attgttttgt tccgcttttt cgagaagtgg ttttctcagc ttctttgggt
      781 agaatatttt gcgatatgtt ttctggtgat catactcagt taatattcca agttgcccta
      841 acacatcgta agcagcggcg ccacatcgca tacccaaaaa cgcatagtca tagggtgttt
      901 gatttaaata cgcattggca atgctatcaa atttttgttt ctgtaaagca cttataggaa
      961 tataaataat tgttttttta acactatccg gatcgcttcc aaacattgca taaaagttat
     1021 cttcagtatg cagtgcaaaa cggctatttc tttcatcctg tttagagaac aaatgaaatt
     1081 cgcctgatgg caggaagttt aatatgtcgt tgctatcgct ttcaatgcca acatgcccac
     1141 ctaaaacacc tccaaaccat ttaggctcgc tttctttata tgcttttaag ggtttggagc
     1201 catataaaaa gtgtaccttg aggtaaatgg tatcctgtga gtaagcgagt aaggatccaa
     1261 ggcaaaatag gagtgtcgtg ataagtttca aggattgtgc gctgtagagt taatggatga
     1321 ctttaatggt atgtgacttc acctcgcttt ttgatgttct tatattttta tactataaaa
     1381 tgtcttttta aatttatcat aataccttct gttatctaaa tcgccacttg tgaaaataac
     1441 tgctgtttta tcctttatta caaaagcatt gaatacaaag ttttgataat tatattgaag
     1501 attggtctct ttgtatgaca aataatagca aacgttgcca ttaagcgtag tatctttttc
     1561 aattttatct gatatggtat aacctgcctt tatagacata ctaagtaact tatagagttg
     1621 tttttttgcc tttttagccg ataggttttc catgatggta aatgcaaagg ccgtaggatt
     1681 ttgagtttga atatctgaag gtccattttt ggtatacact attgtccctt ggaagtcagc
     1741 ggccaaatgg aagcccgtta tggttgtgtc aatttgcaaa tcttcaagtt taagttgtcc
     1801 aaaggaaatg tttgaaatta ggagaaaaat aatgacaatt gatggtttca tggatattta
     1861 gtttttagac aatatttgta aagtgtttat tctgttagcc acaatttcct gcatattatt
     1921 ttccttctcc tttctccaaa tttaagaaat tgatgtgaaa tccattcatc caaaatcgtt
     1981 aactgaccta tgaggttttc acagcaggag cttgttgaaa aattaattag tcttaagtat
     2041 ttagtttatc acacccaaat cctcaccgca cactccacgc caatttctac agcttcctta
     2101 tttctgattt cggctttgag aggtaggagg taggttttgc gtttggtgtc ataccaaatg
     2161 gcagatttcc attctgtgtt gccaattttc gcggttattt gcaagcgccc ccaaccttgc
     2221 tcttcatttt tgaaaatgct ccgtatttca ttggcagtgt ctttcggcaa gtcgacaaat
     2281 acccatgcac cttgtccctg atattgccaa gggactgctt taaatgaaaa tggaacgcct
     2341 ttaaatgcca tcgtatacgg aagaatttga atgcttaagt aatattgaat tcgctttgca
     2401 gtatatcatt gatgctgtcg ctgagcattg caattaattc cggatccata tatgcatact
     2461 catcaggaat atgcaaaacg atgatcttcg ggatatcgcg accaaatatt tcaataatct
     2521 tgtctcgctg atcagattcc atcaccaaca caagatcagc ccacgctaga tcacgcgtat
     2581 gcagcttctt gtcggcactc gcgctcactc cggccgacct gatctcaaaa cgttgatcct
     2641 gcttgaaaat ttcttcagca gttttgctgc gctgcttatt gcgcccgc
//
LOCUS       DAOQHY010000295         2670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_26726_length_2670_cov_4.122753, whole genome shotgun sequence.
ACCESSION   DAOQHY010000295 DAOQHY010000000
VERSION     DAOQHY010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26726_length_2670_cov_4.122753"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            439..2637
                     /locus_tag="PLL28_14745"
     CDS             439..2637
                     /locus_tag="PLL28_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HNF70638.1"
                     /translation="MSTNRTAAMAELLHRELVPIELPASKISEFFGSNVFSDAEMRKH
                     LSEEAYNSIRHSIENGQKIDRKIADQVAASMKAWAMGKGATHYTHWFQPLTGATAEKH
                     DSFFTAVATAVGVETFSGSALVQQEPDASSFPSGGIRSTFEARGYTAWDPSSPAFIMD
                     SVSGKTLCIPTIFVSYTGEALDYKAPLLKSLHLLEEAAVPVCQYFDKNVNRVNATLGW
                     EQEYFLVDEALFLARPDLSICGRSLVGHYPAKGQQLEDHYFGTIPERIYNYMVDFETE
                     AHKLGIPLRTRHNEVAPGQYECAPLFEEASIAVDHNLLLMDVMEKVARRHKLRVLFHE
                     KPFAGVNGSGKHNNWSMGTDTGKNLLSPGKTPRTNLQFLTFFINTIKAVHEYADLLRV
                     SIAAAGNDHRLGANEAPPAIISAFIGTALREVLNEIENRVTEKDFDESDKTDLRLDIH
                     NKIPDLLLDNTDRNRTSPFAFTGNKFEFRAVGSSANCSKAMTTLNTIVATQLIAFRTE
                     VDALIKGGENKDSAILKVLRRYITESKNILFEGDNYSEEWAKEATEKRGLSNVKTTPE
                     AIEFYGTDKAKKVFVSNNILSDRELHARQEIEFEEYAKKIQIEARIYGELVYNYVLPA
                     SITYQNELLDNILKLKAVGLNEAHYKEQLDLAQTITLHINHLRSSLERMVEERKRANH
                     IEHAREKAYAYCNDVKPLFDTIREHADMLERYLPDASWPLPKYRELLFVK"
BASE COUNT          770 a          668 c          566 g          666 t
ORIGIN      
        1 taatgtttct tcttctgatc acatacatcg gaacaagtac atctgcgcgc cggaatcgtg
       61 agtcgtgagt cgtgagtggt gaatcgtgaa tcgtgaatcg taaatggtga atggtaagct
      121 tgcctcgccg agtatcacgc atttcaccac taaccactca caaaaccatc cgtaccacac
      181 tcacgagtca cgattcacga gtcacgataa gcaaagtacc tatcggcgag gcaagctcac
      241 ccacccgcct gctcggcgag gcaagctcac tctctcaccc tctcctctct cctttttcct
      301 ctctccttcg ccaatcaatt tatgtttttc aatatatgtt attttttaac cgaataatca
      361 cttttccaca tccctgtgcc aaaattttca tgttttttaa aaaggaggtt ctaactttgc
      421 ctcaaaatcc accacaaaat gtctactaac cgtaccgctg ccatggcaga actgttgcac
      481 cgcgaactgg tgccaatcga acttcctgcc tcaaaaatct ctgaattctt tggctctaac
      541 gtgttcagcg acgccgaaat gcgcaaacac ctcagcgagg aagcgtataa cagcattcgc
      601 cattcgatag agaacggaca aaaaatcgac cgcaagattg cagatcaggt ggcagccagc
      661 atgaaagcct gggctatggg caaaggcgcc actcattata cccactggtt ccaaccgctt
      721 acaggagcca ctgccgaaaa gcacgacagc ttttttacgg cagtagccac agctgttggt
      781 gtggagacat tcagcggaag tgcattggtg caacaagaac ctgatgcctc cagcttccca
      841 agcggtggta tccgcagtac tttcgaagcg cgtggttata ccgcatggga tccatcatca
      901 ccggcgttca tcatggattc tgtgagtggc aaaacactct gtattccaac aatcttcgtt
      961 tcttataccg gtgaagcgct cgattataaa gcaccacttc ttaaatcatt acatctgctc
     1021 gaagaagctg ccgttccggt ttgccaatac ttcgacaaaa atgttaaccg cgtaaatgct
     1081 accctcggat gggagcaaga atattttttg gtagatgaag cactgttcct cgcccgtccc
     1141 gatttgagca tttgcggtcg ttcattggtg ggccactacc ctgctaaagg tcagcagttg
     1201 gaagatcatt acttcggaac tattccggag cgtatatata attatatggt ggatttcgaa
     1261 accgaagcac acaaactcgg tatcccactg cgtacgcgtc acaatgaggt ggctcccgga
     1321 caatacgaat gtgcgccatt atttgaagaa gcatccattg ccgttgatca taacttatta
     1381 ttaatggatg tgatggaaaa agtagcgcgt cgccataaat tgcgtgtatt attccacgaa
     1441 aaaccatttg caggtgtcaa cggctcagga aaacacaata actggtcgat gggcaccgat
     1501 accggcaaga atttgctgag tccgggaaaa acaccacgca ccaatcttca atttctcacc
     1561 ttttttatca atacgattaa agccgttcat gagtatgccg acctgctccg tgtttctatt
     1621 gccgctgcag gaaacgatca tcgccttggt gccaacgaag cacctccggc aattatttct
     1681 gcattcatcg gaacagcctt gcgcgaagta ttgaatgaaa ttgaaaatcg cgttactgaa
     1741 aaagattttg atgagtcgga taaaacagat cttcgcttag atatccataa taaaattccg
     1801 gacctgctcc tcgacaatac agatcgcaac cgcacaagtc catttgcatt caccggtaat
     1861 aaattcgaat tccgtgcagt aggttcaagt gctaactgct ctaaggcgat gactacgttg
     1921 aacactatcg ttgctacgca attaattgcc ttccgcactg aagtggatgc cttgataaaa
     1981 ggtggtgaga ataaagacag cgcgatattg aaagtattgc gtcgctacat cacggaaagt
     2041 aaaaacattt tattcgaagg tgataattac agcgaagaat gggcaaaaga ggcgacagaa
     2101 aaacgtggac tcagcaatgt taaaaccaca cctgaggcta tcgaattcta tggcaccgac
     2161 aaagcgaaaa aagttttcgt ttccaacaat atcctcagcg atcgcgaatt acatgcacgt
     2221 caggaaattg aatttgaaga atacgccaag aaaattcaaa tcgaagcgcg tatttatggc
     2281 gaattggtat acaactatgt attacccgct tccatcactt atcaaaatga gttgctcgac
     2341 aatatcctca aattaaaagc agtaggattg aacgaagccc attacaaaga acagctcgac
     2401 ctcgcacaaa ccatcacgct gcatatcaat cacctgcgtt cttcactcga acgcatggta
     2461 gaagaacgca aacgtgccaa ccatatcgag cacgctcgcg aaaaagcata tgcttattgc
     2521 aatgacgtga aaccactctt cgacaccatc cgcgagcacg ccgacatgct cgaacgctac
     2581 ctccccgatg cctcatggcc attgccgaag tatcgtgagt tgttgttcgt gaagtgaggt
     2641 cagtgggtga gcttgcctcg ccgataggcg
//
LOCUS       DAOQHY010000296         2618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_27633_length_2618_cov_4.807647, whole genome shotgun sequence.
ACCESSION   DAOQHY010000296 DAOQHY010000000
VERSION     DAOQHY010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27633_length_2618_cov_4.807647"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PLL28_14750"
     CDS             <1..365
                     /locus_tag="PLL28_14750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70639.1"
                     /translation="EITPLHTYGGIGDYIVSLDVVKYYGCAAMTYSPIQVVVHVDDIE
                     NADITVMPNPATQVVNISGLQSAASIEILDITGKVVSTLFTESEIDISAFPAGQYYLR
                     ITNKDVVRVLTFVKVDMQ"
     gene            complement(424..852)
                     /locus_tag="PLL28_14755"
     CDS             complement(424..852)
                     /locus_tag="PLL28_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HNF70640.1"
                     /translation="MYPKELVMPMKAELTAVGFEDLSTPELVTNAIKNTEGTYLLVFN
                     SVCGCAAGSARPGVKMSLANEVKPDHIGTVFAGYDMEAVAEARKLTLPYPPSSPSVAL
                     FKNGKLVYFMERHMIEGRNAMMIAAELANAYNKHCVQVEG"
     gene            1029..2345
                     /gene="ahcY"
                     /locus_tag="PLL28_14760"
     CDS             1029..2345
                     /gene="ahcY"
                     /locus_tag="PLL28_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNF70641.1"
                     /translation="MSVITDKKLPYKVKDITLAEWGRKEIRLAEAEMPGLMAIREEFG
                     ASQPLKGARIAGCLHMTIQTAVLIETLKELGAQVQWSSCNIFSTQDHAAAAIAAAGIP
                     VYAWKGMSLEEYDWCIEQTLFFGENNEPLNMILDDGGDLTNVVLDKYPELIKHVKGIS
                     EETTTGVHRLYERMAKGTLPMPAFNVNDSVTKSKFDNKYGCKESLVDAIRRATDVMMA
                     GKVAVVAGYGDVGKGSAASLRGSGARVLVTEIDPICALQAAMDGYEVKKMEDAVREAD
                     IVVTATGNCDIITERHFRLMKHNAIVCNIGHFDDEIDVAWLNNNYGNTRDTIKPQVDK
                     YTIEGKDILLLAEGRLVNLGCAMGHPSFVMSNSFSNQTLAQIELWVNGNKYENKVYTL
                     PKHLDEKVARLHLAKLGVVLEELNDKQANYIGVEKAGPYKPEYYRY"
BASE COUNT          800 a          529 c          563 g          726 t
ORIGIN      
        1 ccgaaataac tccattacat acatacggtg gaattgggga ttatattgtc agccttgatg
       61 tggttaaata ttatggttgc gctgcaatga cctattcacc aatacaagtt gtggtacacg
      121 ttgatgatat tgaaaatgct gatatcactg taatgccaaa tcctgctaca caggttgtca
      181 atatttcagg attgcagtca gcggcatcta ttgaaatatt ggatattaca ggaaaggtag
      241 tttcgacatt atttacagaa agtgaaatcg atatcagtgc ttttcctgca ggccaatatt
      301 atttacgcat tacgaacaaa gatgttgtac gcgtactgac atttgtgaaa gttgacatgc
      361 aataaaaaat ccgtcatcgg aataggtatg cattccaatg acggatgtat aatctgaaaa
      421 aaattatcct tctacctgca cacaatgctt gttataagca tttgccaatt cagcagcaat
      481 catcatggca ttgcgacctt caatcatatg tcgctccatg aaatacacca gtttgccatt
      541 tttaaataaa gccacactag gtgaacttgg agggtagggt agagtaagct tgcgagcttc
      601 ggctacggct tccatatcat atccggcgaa aacggtgccg atatgatcgg gttttacttc
      661 attggcaagc gacattttta ctcccggacg cgcactgccg gcagcacagc cacatactga
      721 gttaaacacc agcaggtagg tgccttctgt gtttttaata gcgttggtta ccaattcagg
      781 agtcgacagg tcttcgaagc ctacagccgt aagttcggct ttcattggca ttacgagttc
      841 ttttggatac atgtgcgaaa atgttaatta tgctgtaaaa ttaatgctta tcatgctgaa
      901 agccgctata tgacgattct ttcatttcat ccatgaacca tattggcagc taaatggttc
      961 atacgttgcc cgacctgaag caattgatta cctttgcagg gcgtttaaac taatattaaa
     1021 caataaacat gagcgtcatt acagacaaaa aattacctta taaggtaaaa gacattaccc
     1081 tggccgaatg gggtcgtaaa gaaatcagac tggcagaagc agaaatgccg ggattaatgg
     1141 ccatccgcga ggaatttgga gcctctcagc cattgaaagg tgcacgcatc gcaggatgcc
     1201 tccacatgac cattcaaact gccgtattga tagaaacgct taaagaactc ggcgcacagg
     1261 tgcaatggag ttcttgcaac atcttctcta cccaagatca cgccgctgcc gctattgctg
     1321 ctgcaggtat tccggtgtat gcatggaagg gaatgtcgct ggaagaatat gattggtgta
     1381 ttgaacaaac cctgttcttc ggagaaaata atgaaccgct gaatatgatt ctcgatgatg
     1441 gcggcgacct taccaatgtt gtgttggata aatatcctga actcattaaa catgtgaaag
     1501 gtatcagtga agaaactact accggtgttc accgtctata tgaacgtatg gccaaaggca
     1561 ccctgccaat gcctgctttc aatgtgaacg acagtgttac aaaatcgaaa ttcgataata
     1621 aatacggttg taaagaatca ctggtggatg ccattcgtcg cgctactgac gtgatgatgg
     1681 ccggaaaagt ggccgttgtt gccggttatg gtgatgtagg taaaggctct gcagcttcac
     1741 tgcgcggttc cggtgcacgt gtccttgtca ccgaaatcga tccgatatgt gcattgcagg
     1801 ccgcgatgga cggatatgaa gtaaagaaaa tggaagatgc cgtgcgcgaa gcagatatcg
     1861 tagttacagc tacaggaaac tgtgatatca tcaccgaacg tcatttccgt ttgatgaaac
     1921 acaacgctat cgtttgtaat atcggtcact ttgatgatga gattgatgtg gcatggttga
     1981 ataataatta tggcaatacc cgcgatacca tcaaaccaca ggttgataaa tataccatcg
     2041 aaggaaaaga tatcctcctc cttgctgaag gtcgtctggt aaaccttgga tgtgcaatgg
     2101 gtcaccctag ctttgtaatg agtaactcat tcagcaacca aacactcgca caaattgaat
     2161 tgtgggtgaa tggtaataaa tatgagaaca aagtatatac acttcctaaa cacctcgacg
     2221 aaaaagttgc ccgtctgcac cttgctaaac tgggtgttgt gctggaagaa ctcaacgata
     2281 aacaagcaaa ttatatcggt gtagaaaaag caggtcctta taaacctgaa tactaccgct
     2341 attaatttat tagatagctt tcgaaagggg gtggaagaaa ttctgccccc ttttttttat
     2401 tgtgactatt cactaataat attattaaat gtaccattat aataataaaa atacaaattc
     2461 attttcaccc ctaaatccac tgtaagtatc aaatatcagg tatcaggtat caagtatcag
     2521 gtatcaagta tcaggtatca agtatcaggt aaaaattcac gagtcacgag tcacgaatca
     2581 cgattcacga ttcagtagca gtagttatct tactcctt
//
LOCUS       DAOQHY010000297         2615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_27682_length_2615_cov_5.317969, whole genome shotgun sequence.
ACCESSION   DAOQHY010000297 DAOQHY010000000
VERSION     DAOQHY010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27682_length_2615_cov_5.317969"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PLL28_14765"
     CDS             complement(<1..575)
                     /locus_tag="PLL28_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNF70642.1"
                     /translation="MIFLDFEKPIADLYEELEKLKAVGEKQKIDISKPEAELNDKIAQ
                     LRKQIYENLTGWQKVQLSRHPDRPYSLFYIEHMCEDFVELHGDRNVRDDKAIIGGMAT
                     LEGQTVMFIGHQKGINTKMRQYRNFGMANPEGYRKAMRLMKLAEKFNIPIVTLIDTPG
                     AYPGLEAEERGQAEAIARNLYEMTTLRVPVI"
     gene            707..1138
                     /locus_tag="PLL28_14770"
     CDS             707..1138
                     /locus_tag="PLL28_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70643.1"
                     /translation="MRIFSIIFASTLVLAGCRQDSKVTHNEGIEHHQFHDEGGLQLDN
                     GKKWQANAETTAGIQIMQERIANFSGNDVDAAALGTALLGDYQTIFDKCTMTGNAHVM
                     LHKYLLPLKPHLDELEACKSSCAEHVVHVKEYLDNYSTYFE"
     gene            1138..2013
                     /gene="prmC"
                     /locus_tag="PLL28_14775"
     CDS             1138..2013
                     /gene="prmC"
                     /locus_tag="PLL28_14775"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNF70644.1"
                     /translation="MSDKRNQIRYADAVGALKAVLIQHYDVNEASAIARIAGEETFGR
                     DAARSNSLLEMEQLRSWDIITAKLSSGMPLQYALGRAWFMDMTLEVNPSVLIPRPETE
                     ELVELAINQLKAEKLGNGCRLLDIGTGSGCIAIAMKRGLQALEVHAIDISDVAIEIAT
                     RNAKRIDADVHYANANILDPSTLSGLGMFDVIISNPPYIAPAEATGMHKNVVDHEPHP
                     ALFTGDDDPLEFYRAIAAFGRKALHKGGYIFCEISALRGEETKNIFTSAGYNEVQLIT
                     DMQGLPRIVTARHAG"
     gene            2134..2210
                     /locus_tag="PLL28_14780"
     tRNA            2134..2210
                     /locus_tag="PLL28_14780"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2168..2170,aa:Asp,seq:gtc)
     gene            2414..>2615
                     /locus_tag="PLL28_14785"
     CDS             2414..>2615
                     /locus_tag="PLL28_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70645.1"
                     /translation="MKIGLKKYKYLIVVLCIAILLIAILLYFRSRIIKQQNIALNSLE
                     QITYSCERKNETLKEFKDLLVEN"
BASE COUNT          752 a          558 c          588 g          717 t
ORIGIN      
        1 caaatcacag gcacgcgcaa agttgtcatc tcatacaaat ttcttgcaat agcttctgct
       61 tgtccgcgct cttcagcttc cagtcccgga taggcccccg gagtatcgat aagcgtgaca
      121 atcggaatgt tgaatttctc tgccagcttc atcaaacgca tcgccttgcg gtaaccttca
      181 ggattcgcca taccaaaatt gcgatactga cgcatcttgg tgttgatgcc cttttgatgg
      241 ccgatgaaca tcaccgtctg accctctaat gtagccatac cgccgatgat agccttgtcg
      301 tcacgcacat ttctatcgcc atgcaattca acaaaatcct cacacatatg ctcgatgtaa
      361 aacaagctat aaggcctgtc ggggtgacgg ctcaactgca ctttctgcca gccggtgaga
      421 ttttcgtata tctgtttgcg caattgcgcg atcttgtcat ttaactcagc ctccggttta
      481 gagatgtcga tcttctgctt ttcacctact gccttcagct tctccaactc ctcatacagg
      541 tcggctatcg gtttttcaaa gtcaaggaat atcataagag caaaggtagg aaatcggaag
      601 gaaacttgaa tggcggtgac tgttgtggta tatacaccgc tacaatttgg cttaagatta
      661 ttgagggtct cgtttggcga atgatttggt cttaacttta cacattatgc gtatattttc
      721 tataattttc gcttcaaccc ttgttttggc cgggtgcagg caggattcta aagtcaccca
      781 caacgaggga atagagcacc atcaatttca tgatgaggga ggcttgcaac ttgacaacgg
      841 gaagaaatgg caggctaatg cagaaaccac tgccgggatt caaatcatgc aggagcgcat
      901 cgcaaatttt agtggcaacg atgtggatgc tgcagcacta ggtacagcat tattaggcga
      961 ctatcagacc attttcgaca aatgcaccat gaccggcaat gcacatgtca tgttacacaa
     1021 gtatctgctg cccttaaagc ctcaccttga cgaattagag gcctgtaaat catcctgcgc
     1081 tgaacatgtt gtgcacgtga aggaatatct cgataattat tccacttatt tcgaataatg
     1141 tcggataaaa ggaatcaaat ccggtatgca gatgctgttg gggcgctcaa agccgtacta
     1201 atacaacatt acgatgtaaa tgaggcgtct gcaattgcgc gaattgcagg cgaggaaacc
     1261 tttgggaggg atgctgcgcg aagtaattcg ctcttggaaa tggaacagtt gagaagctgg
     1321 gatattatca ccgcgaaatt gagttccggt atgcctctgc aatatgcgct aggcagagcc
     1381 tggttcatgg atatgacatt agaagtgaac ccatcggtgc tgattccacg cccggagacc
     1441 gaagagctag tagaacttgc cataaaccag ttgaaagccg agaaattagg taacggttgt
     1501 cgcctcctgg atatcggtac aggaagcggg tgcattgcca ttgccatgaa aagaggttta
     1561 caggcattgg aagtgcatgc gatcgacatc tctgatgtag ctatagagat tgcaacccgc
     1621 aatgccaagc gtatagatgc agatgtgcat tatgcgaacg caaacatcct tgatccgtcc
     1681 acactcagtg gtttaggtat gtttgatgtg atcatcagta atccgccata tattgcaccg
     1741 gctgaagcta ccggcatgca taagaatgta gttgaccacg aaccgcatcc ggccttgttt
     1801 acaggggatg atgatccact tgagttttat cgcgccatcg ccgcatttgg aaggaaggcg
     1861 ttgcataaag gagggtatat tttttgcgag atatccgcct tgcgtggaga ggaaactaaa
     1921 aacattttca catcagccgg atacaacgaa gtgcagctta tcaccgacat gcagggctta
     1981 ccgcgcatag ttactgcgcg acatgcaggt tagttttttt cgggactatt tttcgaattc
     2041 atggaaaagg ttatatattt gcatctcttt ttgagcaaat acgcagtgca aactgcaaaa
     2101 tgcccacaaa aagattgcgt tcttaaaccg cacggagcgg tagttcagtt ggttagaata
     2161 cgtgcctgtc acgcacgggg tcgcgggttc gagtcccgtc cgctccgcca ctattatctt
     2221 gaaaccctta tagatcaatg atttacaagg gtttttgctt tttaggcgaa acatgggcga
     2281 aacaaagttt gaaataaata aaagtgacta tctttaataa actcccaaaa agctagatag
     2341 cgagaggagt atcattggtt attcaagaat aacagaagca tttaattata ttcctataaa
     2401 aaaaattagg attatgaaaa ttggtttaaa aaagtacaag tatttaatag tggttctctg
     2461 tattgcgata cttctcattg ctatactctt atattttcga tctagaatta ttaaacaaca
     2521 aaatattgca ttaaactcac ttgaacaaat tacatattca tgtgaaagaa aaaatgaaac
     2581 tcttaaggag ttcaaagatt tacttgtaga aaata
//
LOCUS       DAOQHY010000298         2608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_27811_length_2608_cov_4.455934, whole genome shotgun sequence.
ACCESSION   DAOQHY010000298 DAOQHY010000000
VERSION     DAOQHY010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27811_length_2608_cov_4.455934"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..1404)
                     /locus_tag="PLL28_14790"
     CDS             complement(178..1404)
                     /locus_tag="PLL28_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNF70646.1"
                     /translation="MPHISQRGADMPASPIRKLVPFAEKAKLRGVKIYHLNIGQPDIV
                     TPPQMLDAVKRTDLKVIEYSHSAGFESYRRKLVTYYESRGIHINHHQIMITTGGSEAI
                     LFGMMSCLDAGDEVIIPEPLYANYNGFSTAGNIVIKPITSHIENGFALPPIAEFEKLI
                     TPKTKAILICNPNNPTGYLYSKEELMQLRDLILKYDLFLFADEVYREFVYDGKEHFSV
                     MNLDGLDEHVVLMDSISKRYSACGARIGALISRNNKVMETAMKFAQARLSPPTLEQIL
                     AEAFLDVPETYFQEVGKEYVERRDLCVARLNAMDGVMCPTPGGAFYAIARLPIDDADV
                     FCQWLLESFNHNNETVMLAPATGFYSTPGLGKNEVRIAYVLNKQDINKAMDCLDAALK
                     VYPGRTAIIQNAEVNA"
     gene            complement(1549..2004)
                     /locus_tag="PLL28_14795"
     CDS             complement(1549..2004)
                     /locus_tag="PLL28_14795"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNF70647.1"
                     /translation="MKKVLSIVAFLAVTGAMTAQTATGTGTTAAVSENAAEFQFVTEV
                     FDFGQIPQGVPAEARFEFTNTGKEPLIITDVQKTCGCTKTDWSKEPIAPGAKGWIIAE
                     YNAANEGSFTKAITVFSNTKTPNMKLTFKGTVVKDESGSVPQQKSIFDN"
     gene            complement(2032..2520)
                     /locus_tag="PLL28_14800"
     CDS             complement(2032..2520)
                     /locus_tag="PLL28_14800"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNF70648.1"
                     /translation="MKKFTYTLALTMLIATASFAQTSTTPPAGSSEATTPVVNPNAGE
                     FQWSEETYDFGTIAQGVPVKHKFEFTNTGKEPIIVSNVVKTCGCTVTDWTKEPVLPGQ
                     KGQVIAEFNAAKEGPFTKAITVQSDAKTPSVKLYFKGTVQKSEQAGSVPENNNIFNQG
                     GN"
BASE COUNT          696 a          556 c          548 g          808 t
ORIGIN      
        1 ttccttccag atcggccgga atttgttgtg aaaatgcagt tgccgataat aacaggcaaa
       61 tggaaaggca taaggtacgc atgatgtaat attttgtaca aaagtatcgg caaaagccat
      121 cacgccccaa cactttagat gaaacccatt aattcttcga cggacgtaag cctgctatca
      181 ggcgtttact tcggcatttt gaatgattgc ggttctgccg gggtaaactt ttaacgcagc
      241 atccaagcaa tccattgctt tatttatatc ctgtttattc aacacataag caatacgcac
      301 ttcatttttt cccaatccgg gagtagaata aaatccggtt gcaggtgcca gcatcactgt
      361 ttcattattg tgattgaatg attccagcag ccactgacaa aaaacatcag catcgtcgat
      421 aggtaaacgt gcaatagcat aaaatgcacc acccggagtt gggcacatta cgccatccat
      481 tgcatttaat cgcgcaacac agagatcgcg gcgctctaca tattctttcc ccacttcctg
      541 aaaatatgtt tccggtacat caaggaatgc ttcagcaaga atttgttcca atgtaggagg
      601 tgataaacgc gcttgtgcaa atttcatggc tgtttccatc actttgttat tgcgtgatat
      661 taacgcacca attcgcgctc cgcaagcact gtatcgttta gaaatagaat ccatcagtac
      721 cacatgctca tccagtccgt cgagattcat caccgaaaaa tgttcctttc catcgtacac
      781 aaattcgcga tagacttcat cggcaaaaag gaacaaatca tattttaaaa taagatcgcg
      841 caattgcatt aattcttcct tgctatataa atatcctgtc ggattattcg gattgcaaat
      901 aagaattgcc tttgttttcg gcgtgatcag tttttcaaat tccgcaatcg gtggcagtgc
      961 gaaaccgttt tctatatgtg aggtaattgg tttgatcaca atatttcctg ccgtgctgaa
     1021 accattgtaa tttgcatata acggttcggg aataatcact tcatccccgg catccaaaca
     1081 actcatcata ccgaataaaa tggcttcgct gcctccggta gtgatcatga tctgatgatg
     1141 attaatatga atgcctctgc tttcgtaata agtcaccaat ttgcgacgat aagattcaaa
     1201 tcctgcagag tgactgtatt caatcacttt caggtctgtg cgcttaacgg catccagcat
     1261 ttgcggaggt gtaacgatat ccggctgacc gatattcaga tggtagattt ttacgcctct
     1321 gagttttgct ttttctgcaa atggaaccag tttgcgaatc ggtgaggcag gcatatcagc
     1381 ccctcgttgc gaaatatgtg gcataaggca aaggtaggcg gtttttggga aatgaacgcc
     1441 ctgtgattca aggaggggct tgtaagttct tgcagctcaa tcaactgcaa aaaaaagagg
     1501 ccgccttttc agacagcctc cttttcgcag ataacgttca ggtaaatatc aattatcgaa
     1561 aatcgatttt tgctgaggaa cgctgcctga ttcatctttt accactgtgc ctttgaaggt
     1621 aagcttcatg tttggcgttt tggtatttga gaaaacagtg atggctttag taaatgagcc
     1681 ttcatttgca gcattatatt cggcgatgat ccatccttta gcacccggag caataggttc
     1741 tttactccaa tcggttttgg tacatccgca tgttttttga acatcggtga tgatcaacgg
     1801 ttctttacct gtgtttgtga attcgaaacg cgcttcagca ggaactcctt gtgggatttg
     1861 accgaaatca aaaacctccg taacaaactg gaattcagca gcattttcgc ttactgcagc
     1921 ggtagttcct gtgcctgtag ctgtttgtgc cgtcattgcg ccggtaacag ctaaaaatgc
     1981 aacaatcgat aatacttttt tcatgttctt taatatttag gctgtggatg atcagttacc
     2041 accctggtta aaaatattgt tgttttcagg aacgcttccg gcttgttctg atttttgaac
     2101 agtgcctttg aagtagagtt tcactgatgg tgtttttgca tctgactgca cagtgatggc
     2161 ttttgtaaac ggaccttctt ttgcagcatt gaattcagca attacctgac ctttttgtcc
     2221 gggtaataca ggttcttttg tccagtcagt aactgtacaa ccacatgttt ttaccacgtt
     2281 acttacaata ataggttctt taccggtatt ggtgaattcg aatttatgtt tcaccggaac
     2341 gccttgagcg atagtgccga agtcgtaagt ttcttcagac cattgaaatt cgcccgcatt
     2401 aggatttact accggagtgg tagcttctga tgatcctgca ggtggtgttg ttgaagtttg
     2461 cgcaaatgaa gctgttgcga tcagcatcgt caaagcgagt gtgtaagtaa attttttcat
     2521 attcaaattg gtttttatta cggttggtca atagctgctt gccgaccggg aggccaaatc
     2581 taagcaactg ccgcaaatat ttgcagcc
//
LOCUS       DAOQHY010000299         2545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_29028_length_2545_cov_4.787149, whole genome shotgun sequence.
ACCESSION   DAOQHY010000299 DAOQHY010000000
VERSION     DAOQHY010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29028_length_2545_cov_4.787149"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2545)
                     /locus_tag="PLL28_14805"
     CDS             complement(<1..>2545)
                     /locus_tag="PLL28_14805"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF70649.1"
                     /translation="TYITAFTIANNALIIGTPTGIKEFIYDNTADASGAQNRMYRETP
                     VQFPGFTSTSRINALYSDPSGDWIAADENGNILIHNKKPQVIRIPEIPEYAIITAISS
                     DAENNIWVGTDRAGIFIWNNGLKAHYTSDSLIISNAITALYRDAQHAIWIATMDAGVM
                     KYENDKFTAFTYFDNGLISNNISCITQDANGMMWFGSPDEGICSFDGSGFAFYTDNDV
                     LTSNNVNGITFDIQGNLWVALNDGVDHLTFNADSSITTKHFDAYDGFTGIRNNRNTII
                     ATTDGHVWFGTVNGLFRYNPEEDVFSITKPVIELRNIRLYYENPDWALYSDSIGGWYN
                     LPVNLHLPYDQNHVTFDFSAIFFSVHEKIQYAVMLEGFDDTWQNIGTGTSMTYSNLKP
                     GNYTFQVRAQNADGVWSDPVTYKFSISKPFWLTWWFIVMAVVVGLSIIAILFWWRNRR
                     FRIQAKLLEQTVMERTAELENQKVMAEKAAWRAERSEKAKEEFLANMSHEIRTPMNAI
                     MGMTRLLLEKEPKSEQLKYLKAIRQSSDNLLVIINDILDLSKIQAGKMELEKIPFNVR
                     SVLNNMAEIMKFKSDEKKLDFKVIIDPAVPEFITGDQVRLNQILINLTGNAIKFTDHG
                     HVYVYCKAVSINENTATVSFDVEDTGIGIAEDKLNSIFESFSQADVATTRKFGGTGLG
                     LSISKRLTELAGGEMRVSSTEGKGSVFSVVIPYPIAEEIASSSEEDHAADIAIATNLQ
                     ARILLVEDNMFNQMVATDSINDMFPKAELVVADNGQIAVDKIVAEQFDVVLMDVQMPV
                     MDGYTASRTIRALQDEQKSKIPIIAMTASVIKSEVDKCYESGMDDFIAKP"
BASE COUNT          686 a          537 c          510 g          812 t
ORIGIN      
        1 ggtttggcaa tgaaatcatc catgccgctt tcataacact tatctacttc acttttaatt
       61 actgatgcgg tcatggcgat aatggggatt ttgctttttt gttcatcttg caaggcgcga
      121 atggttctgg acgcagtata tccatccatc acaggcattt gtacatccat aagcaccaca
      181 tcaaattgtt cagccacaat tttatcgacg gcaatttgac cattatcggc aacaacgagt
      241 tccgctttcg ggaacatgtc gttgatgctg tcggtagcta ccatctggtt aaacatattg
      301 tcttccacca acaaaatgcg cgcttgtaaa tttgtggcga tggcaatatc ggcagcatga
      361 tcttcttcac tgctacttgc aatctcttct gctatcggat atggtattac tacgctgaac
      421 actgagcctt ttccttcagt gcttgaaaca cgcatttcgc cacctgcaag ttctgtgagg
      481 cgtttgctga tacttaatcc caatccggtt ccaccaaatt tgcgcgtggt agctacatca
      541 gcctgcgaga atgactcgaa tatactgttt aatttatctt ctgctatgcc tattcccgta
      601 tcttctacat caaaggaaac agtagctgta ttttcattta tggaaacagc tttgcaatac
      661 acatatacat gtccatggtc ggtgaattta atggcatttc ctgtcagatt tatcaatatt
      721 tgatttaatc gcacctgatc gccggtaata aattcgggaa ctgcagggtc aatgattact
      781 ttgaagtcta atttcttttc gtctgattta aatttcatga tctcggccat attattgagc
      841 actgaccgta cattaaacgg aattttttcc aattccatct ttcctgcctg aatttttgat
      901 aagtcgagaa tatcattgat gatcaccaac agattatcag aagattggcg aatagctttt
      961 agatatttta gttgttccga tttaggttct ttttcgagca gcagtcgcgt catacccata
     1021 atggcattca taggtgtgcg gatctcatga ctcatgttgg cgaggaattc ttcttttgct
     1081 ttttcagatc tttcagcacg ccaggcggct ttttcagcca tcactttttg attttccaat
     1141 tcggcggtgc gttccattac agtctgctcc aataattttg cctgaatgcg gaatcttctg
     1201 tttcgccacc agaataaaat ggcgatgatg ctcaatccaa ctacaacagc catcacaata
     1261 aaccaccatg taagccagaa tggtttgctg atagaaaatt tatatgtgac aggatcactc
     1321 catacgccgt cagcattttg tgcacgtacc tgaaatgtat aattgcccgg tttgaggttt
     1381 gaataagtca tggaagttcc ggtaccgata ttctgccatg tgtcgtcgaa gccttcgagc
     1441 attacggcat actgaatttt ttcatgcacc gaaaagaaaa tggcactaaa atcgaaggtg
     1501 acatgatttt gatcgtaggg gaggtgtaga tttaccggca aattatacca accgcctatt
     1561 gaatcgctgt atagggccca atccggattt tcataataca atctgatatt gcgcaattca
     1621 atcacaggct tggtaatact gaaaacgtct tcctccggat tatagcgaaa taatccattc
     1681 acagtaccga accatacatg gccatcggta gtagcaatga tggtattcct attatttctg
     1741 atgcctgtaa agccgtcata ggcatcgaaa tgttttgtgg tgatactaga atcggcattg
     1801 aacgtcaggt gatctacacc gtcatttaat gcaacccata aatttccttg tatgtcgaat
     1861 gtaatgccat ttacattatt ggaggtaagc acatcattgt cggtataaaa tgcaaaaccg
     1921 ctaccatcaa aactgcaaat accttcatca ggacttccga accacatcat tccgtttgca
     1981 tcctgcgtta tgcatgaaat attattgctg atgaggccat tatcaaaata ggtaaatgcc
     2041 gtgaatttgt cattttcata tttcatcaca ccggcatcca tagttgctat ccagatagca
     2101 tgttgtgcat cgcgataaag tgcggtgata gcatttgaga ttattaagct gtcgctggta
     2161 taatgtgctt ttaatccgtt attccagata aaaattcctg ctctgtcggt gcctacccaa
     2221 atattatttt ctgcgtcaga agagattgca gtaataatgg catattcagg aatttcagga
     2281 atacgaatta cctgcggttt tttattgtgg atgagaatat ttccattttc atctgccgca
     2341 atccaatcgc ctgatggatc gctatagagg gcgttgatgc gagatgtaga agtgaacccg
     2401 ggaaattgaa caggcgtttc gcgatacatg cgattttgcg ccccggaggc atcggcagta
     2461 ttatcgtaaa taaattcttt tattcctgtt ggtgtgccaa tgatcaaggc attgttggca
     2521 atagtgaatg cagtgatgta tgtat
//
LOCUS       DAOQHY010000300         2530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_29307_length_2530_cov_4.123636, whole genome shotgun sequence.
ACCESSION   DAOQHY010000300 DAOQHY010000000
VERSION     DAOQHY010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29307_length_2530_cov_4.123636"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1414
                     /locus_tag="PLL28_14810"
     CDS             <1..1414
                     /locus_tag="PLL28_14810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70650.1"
                     /translation="NGYNVTQFYSADINPLVANQQVMGGTQDNGTPYAYDPGVADFKM
                     VSGGDGAFVAFNTNDADKFYTASQLNKLFRFNNGGFETPDTITNPNLNDGSVQFINPF
                     ALDASDPEVLYWTSKAGVWRLKNASTADTGQWQKAANLNGVISAIGSSPAAPGVLFIG
                     RSSGVGDIYRLDDAYNSDLTVVPFNTDLRDSLPDAPFTGSVYCSSIVVDHHDANHVVA
                     TYSNYNLESVWECENALSPDPIWTCIEGDLPDIPVYWSAIHPQFPDIIYIATEIGVFY
                     TMLADGANTVWIPCASFPAVRTDMLKVRTNDGIIVAATHGRGIWQATLDHHGLLIDPF
                     WSERGPANVGGRTRTIMVDPNDPTGKTVWAGSIAGGLWKTTDIDAVPVYNQTASTFTL
                     SVSPNPASSIIRINLSGTSSKGATLTMYRMDGSVAAVIGKSQLSGQVSWQIPSGLSAG
                     TYLLVATDGQNRAVQRVIVL"
     gene            1485..2459
                     /locus_tag="PLL28_14815"
     CDS             1485..2459
                     /locus_tag="PLL28_14815"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HNF70651.1"
                     /translation="MREIRFREALREAMVEEMRKDQHILLMGEEVAEYNGAYKVSQGM
                     LDEFGAKRVIDTPIAELGFAGIGVGAAMNGLRPIVEFMTFNFALLAIDQVVNSAAKML
                     AMSAGQFRCPIVFRGPSGSAGQLGAQHSQMFENWYANVPGLKVISPSNPYDAKGLLKS
                     AIIDDDPVIFMESELMYSDMGAVPEEEYYIPIGKADVKRSGSDITIVSFNKMMKVALK
                     AAEELEKDGISAEVIDLRTIRPLDINTIVESVKKTNRIVVVDESWPYGSIASEIAYQL
                     QKDAFDYLDAPVRRVNGADTSMHYAPNLVDAYLPNPKKVIEVVKEIVY"
BASE COUNT          646 a          479 c          691 g          714 t
ORIGIN      
        1 caatggatat aatgtcacac aattttattc tgccgatatc aatccgcttg ttgccaatca
       61 gcaggtgatg ggtggcacgc aggataatgg cacaccgtat gcatacgatc ccggtgtggc
      121 tgattttaaa atggtatcgg gtggtgatgg cgcttttgtg gcattcaaca caaatgatgc
      181 ggataaattt tacacggcct cgcaattgaa taaattattt cgatttaata atggcggatt
      241 tgaaacgcct gatactatca cgaatccgaa tttaaatgat ggcagtgtgc aattcatcaa
      301 tccgtttgca ttggatgcgt cggatcctga agtgttatat tggacatcga aggcgggtgt
      361 gtggcgattg aaaaatgcgt cgacagcaga taccggtcag tggcagaaag cggcgaatct
      421 gaatggtgtg atttccgcca tcggatcatc gccggcagct ccgggtgttt tatttatcgg
      481 aagaagttca ggtgtaggcg atatttatcg attggatgat gcatataatt ccgatctcac
      541 tgttgttcct ttcaacaccg atcttcgcga ttcattgcct gatgcaccat ttaccggcag
      601 tgtatattgc agcagtatag tggttgatca tcacgatgcg aatcatgtgg ttgccacgta
      661 ttcgaattat aatcttgaaa gtgtttggga atgtgaaaat gcattatcgc cggatccgat
      721 ttggacatgt attgaaggtg atcttcctga tattcctgtt tattggagtg cgatacatcc
      781 gcagtttccc gatatcattt acattgccac ggaaatcggt gttttttata ccatgcttgc
      841 tgatggcgcc aatacggttt ggataccatg tgcgagtttc cctgcagtgc gcaccgatat
      901 gttgaaggtg cgtacgaacg acggcatcat cgtagcggca acacatggtc gcggtatctg
      961 gcaagcgaca ttggatcatc atggtttgtt gatagatccg ttctggtcgg aacgcggacc
     1021 tgcgaatgtt ggtggcagaa cgcgcaccat catggtagat ccgaatgatc cgacaggaaa
     1081 aacggtatgg gcaggaagta ttgcgggtgg attgtggaag acgacagata ttgatgcggt
     1141 tcctgtttac aatcaaacgg catcgacatt tactttgagt gtatcgccga atcctgcgag
     1201 cagtatcatc cgcataaacc tgagtggtac ttcatctaaa ggcgcaacgc tgaccatgta
     1261 tcgcatggat ggttcggtgg ctgcggtgat agggaaatcg caactttcgg gacaggtaag
     1321 ctggcagata ccatcgggtt tatcggcggg cacttatctg ctggtggcta cggatggcca
     1381 aaacagggcg gtacagcggg ttatcgtgct ataaacctgt cgcggatccg gactgccatg
     1441 ctgtgccgaa catttcctaa ttttgcagca aatttcagca tacaatgaga gagatccgct
     1501 ttcgtgaggc tttgcgtgag gctatggtag aagagatgcg caaagaccag catatcctgt
     1561 tgatgggcga agaggttgcc gaatacaacg gtgcctataa agttagtcag ggcatgttag
     1621 atgagtttgg tgccaagcgc gtgatagata cacctattgc agaacttgga tttgcaggta
     1681 tcggcgttgg tgctgcgatg aatggattgc gtcctatcgt ggaattcatg acattcaatt
     1741 ttgcattact cgcaattgat caggtggtga actctgcagc aaaaatgttg gcgatgagtg
     1801 cgggacagtt tcgttgtccg attgtgtttc gtggacctag cggttctgcg ggacaattag
     1861 gtgcgcaaca ctcgcagatg tttgaaaatt ggtatgcgaa tgttccgggg ttgaaggtga
     1921 tatcgccttc gaatccgtat gatgcgaagg gattattaaa atctgccatc attgatgatg
     1981 atccggttat tttcatggag agtgaattga tgtattccga tatgggtgca gtgcctgagg
     2041 aggaatatta tattcctatc ggtaaagcgg atgttaaacg cagcggaagt gatattacca
     2101 ttgtttcatt taataaaatg atgaaggtgg cattaaaggc tgctgaggaa ttagaaaaag
     2161 acggcatcag tgccgaggta atagatcttc gtactattcg tccgctcgac atcaacacca
     2221 ttgttgagtc ggtgaaaaaa acaaatcgca ttgtggttgt tgatgagagc tggccttatg
     2281 gaagcattgc atctgagatt gcttatcaat tacaaaaaga tgcatttgat tatttagatg
     2341 cgcctgttcg ccgagtgaat ggtgcggata cgagcatgca ttatgcaccg aatctggtag
     2401 atgcttattt gccaaatccg aagaaggtga ttgaggtggt gaaggagatt gtgtattgat
     2461 ttttttcacg cagaggcgca gaggcgcaga ggggtgagtg attactacgt cttgcacact
     2521 tgcacacttc
//
LOCUS       DAOQHY010000301         2499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_29942_length_2499_cov_6.219313, whole genome shotgun sequence.
ACCESSION   DAOQHY010000301 DAOQHY010000000
VERSION     DAOQHY010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29942_length_2499_cov_6.219313"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..1112
                     /locus_tag="PLL28_14820"
     CDS             159..1112
                     /locus_tag="PLL28_14820"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HNF70652.1"
                     /translation="MNKALTKLTMLAVALSMFSCEKTVYFEVETQENLLVVNALIIPD
                     SVNDATISLSVDPIAIGFDAASVSDATVDVYRNDVLSGTFISEGGGYYTLPPTVLAGA
                     DGEEVRFEVSAPGRESVSATTTIPTLVPITSLEITDTLYETISYSVIDDEGNVTYIDT
                     IVPYFNLELTFDDPIGEDHYMLDIDYQDAFSYVQACYTTNDPVFTVESSYGYGGANDD
                     GSVTFCGDVVFSDQSFEGKTKTISALVYAIETTFTIDPTFVVTLKHISDDYYKYYTTS
                     QQQYYNNGDPFSEPTVVFDNITGGFGIVAGYTVSKSTLPLD"
     gene            1115..1468
                     /locus_tag="PLL28_14825"
     CDS             1115..1468
                     /locus_tag="PLL28_14825"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNF70653.1"
                     /translation="MAYDTQLADRIYEILLAKEIPFTEKKMFGGIAYMVNDAMACGII
                     KNDLMIRCFHEDHEKLLKMQHVGEMKFTGKSMRGFLMIYPSGFETDAALGYWLQHGVN
                     YALLAPKKPKKSKKK"
     gene            1551..2261
                     /locus_tag="PLL28_14830"
     CDS             1551..2261
                     /locus_tag="PLL28_14830"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNF70654.1"
                     /translation="MRSQLLFGIAICMLTITHLHAQTPQVDPAYIEVTGSAEMEIIPD
                     EIYISVSLSERYDGREKVTLAVQDEKFFNAIKELGIPMGDVQLSDAVTDYSYYKWRKE
                     DALATKDYIVLVHTAEMTSKLFKKLVDIDAENAKVTKVSHSKIEQYRKEVKIKAMKAT
                     HEKAEYMLEAIGQELGKPIVITELNENVLTTNYAQYDMNSYRTANFMLADGYTMPAEI
                     SFQKIKLKYEIYGKFEIK"
BASE COUNT          800 a          410 c          565 g          724 t
ORIGIN      
        1 caaatgtttc atgggaattt aaattttagg gaaagaggag aaaggagaga ggaaagagga
       61 gaaaggagag aggaaaaagg agaaaggaga gaggaaaaag gagaaaggag agagggcttg
      121 aggttttttt tacaggacac gataataaga taaatacaat gaataaagcg ctgacaaaat
      181 taacgatgct agcagttgca ttgagcatgt ttagctgcga gaagactgtg tattttgagg
      241 tagaaacaca ggaaaacctg ttggttgtga atgcattgat cattcccgac agtgtaaatg
      301 acgcaacgat ttctttgagt gtagatccta ttgcaattgg ttttgatgct gcctcagttt
      361 cagatgctac cgttgatgtg tatcgcaatg atgtgttgtc aggtacattt atttcagagg
      421 gcggaggtta ttatacactt cctccgacag tattggcggg tgccgacggc gaagaagtgc
      481 gttttgaggt aagtgctccc ggtcgcgaat cggtatcagc taccacaact attcctacat
      541 tagtgccaat tacatcgctt gaaatcacag ataccttgta tgaaacgatt tcgtatagtg
      601 taatagatga cgaaggcaat gtaacttata tcgatacaat tgttccttat ttcaatcttg
      661 aactcacctt tgacgatcct atcggtgaag atcattatat gcttgatatt gattatcagg
      721 atgcatttag ctatgttcag gcatgctaca ctaccaacga ccctgtattt actgttgagt
      781 caagttatgg ctatggcgga gcaaatgatg atggcagtgt aactttttgc ggtgatgtag
      841 ttttttcaga tcaatcgttt gaaggaaaaa caaaaaccat tagcgcctta gtatatgcaa
      901 ttgaaactac ctttactatt gaccctacat ttgtggtaac gttgaaacat atttctgacg
      961 attattataa atattacaca actagtcaac aacaatatta taacaacggc gatccgttca
     1021 gcgaacctac tgttgtattt gataatatta ccggcggttt tggtattgtt gcgggttata
     1081 ccgttagtaa atcgacactt cccctcgatt aattatggca tacgatacgc aacttgcaga
     1141 ccgcatatat gaaattttgc tggcaaagga aattcccttt acagaaaaaa agatgtttgg
     1201 gggaatcgct tatatggtaa atgatgctat ggcttgcggc attatcaaaa atgatctgat
     1261 gatcagatgt tttcatgagg atcatgagaa attactgaag atgcaacatg taggtgaaat
     1321 gaaattcacc ggcaagtcga tgcgtggatt tctgatgata tatccttcag gatttgagac
     1381 agatgccgct ttgggatatt ggctgcaaca cggtgtaaat tatgctttgc ttgctcctaa
     1441 gaagcccaaa aagtctaaga aaaagtaaga ttaccctgta ttttgcaata aacagaagtt
     1501 tcatgcgttc gtttcgatgg cggtattgcc attcaataaa aacaggaatc atgcgatctc
     1561 aactactctt cggcattgca atttgcatgc ttacaattac acatttacat gcacaaactc
     1621 ctcaagttga tcctgcttac atcgaagtaa ccggcagtgc tgaaatggaa ataattcccg
     1681 atgaaattta tatttcagtt tctctatctg aaaggtatga cggtcgcgaa aaagtaacac
     1741 ttgccgtgca ggatgaaaaa ttctttaatg cgataaagga attaggtatc ccaatgggtg
     1801 atgtgcagtt atcagatgct gttacagatt atagctatta taaatggcgt aaagaagatg
     1861 cgcttgccac caaagattat attgtattgg tgcacactgc agaaatgaca tccaagctat
     1921 tcaaaaaatt ggttgacata gatgctgaaa atgcgaaggt taccaaggta agtcattcaa
     1981 agattgagca atatcgcaag gaagtaaaaa taaaagcgat gaaggctact catgaaaaag
     2041 cggaatatat gctcgaggcc attggacagg aacttggtaa accgattgta attacggagc
     2101 tgaatgaaaa tgtgttaacc accaattatg cgcagtatga catgaattcc tacaggactg
     2161 ccaatttcat gttagccgat ggttacacca tgcctgcaga gatctctttc caaaaaataa
     2221 aactcaagta tgagatttat gggaagtttg agattaaatg aaaaggagag aggagaaagg
     2281 agaaaggaga aagttggtga gtttacttcg ccgagtaggc gggtgggtga gtttattttg
     2341 cggttgaatg gtattgatag taataaaaaa agccccctga ttgatatcag ggggctttga
     2401 gattgtatat tttttattag gctttttcta cgccttctac tagtacgcta acggtattat
     2461 tcactacttc aacaaagccg cctttgatat tatagacga
//
LOCUS       DAOQHY010000302         2490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_30136_length_2490_cov_4.262423, whole genome shotgun sequence.
ACCESSION   DAOQHY010000302 DAOQHY010000000
VERSION     DAOQHY010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30136_length_2490_cov_4.262423"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1741
                     /locus_tag="PLL28_14835"
     CDS             <1..1741
                     /locus_tag="PLL28_14835"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNF70655.1"
                     /translation="DCGTGNGVVMKALVEGGEIIEALRERILGRVVAIDVVNPENQEI
                     VFAAGELLDEDAVDLIESLGIDEVKVRTPLTCETRYGLCAKCYGRDLGRGTPVNVGEA
                     VGVIAAQSIGEPGTQLTMRTFHIGGAASRTAVVSQVESKSSGIVHYSSTMRYVTNAQN
                     ELIAISRSGEIIIQDENGRERERHKASYGATLMVRNGETVKAGQVLTTWDPHTRPIIT
                     EYSGKVRFENVEEGVTVAKQIDEVTGLSTLVVIDPKRRGVLQSKGLRPLVKFLDDDGN
                     EIKMVGSNQSVSITFQIGCIITVRDGQQVSVGEVLARIPQETSKTRDITGGLPRVAEL
                     FEARSPKDAGMLAEVTGIVSFGKDTKGKQRLVITDLEGIVHEYLIPKDKHVMAHDGQV
                     VNKGEIIVDGPADPRDILRLQGVEALARYITDEVQDVYRLQGVRINDKHIEVIVRQML
                     RRVQVINAGDSSFIPGEQVERADLLIENERLIAEKKMPATYEFLLLGITKASLSTDSF
                     ISAASFQETTRVLTEAAIMGKKDDLRGLKENVIVGRLVPAGTGLSFHSKRRNQEKIFE
                     SGLESDISIDSTV"
     gene            1980..>2490
                     /locus_tag="PLL28_14840"
     CDS             1980..>2490
                     /locus_tag="PLL28_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70656.1"
                     /translation="MKGKLWPKIVAAMLGGLLIGAAHANIPSVPNELYEALKLDREKV
                     TPKELHEALVKRYKDPAQGSGRGTLAQYWEPIPYGIYLDPNTFYKSPTTNKEVASRKE
                     CVECHTDESPVWVQAWKRSSHANLDKVRNLKPGDPTFYKKAKLEDIEKNLRSMGRLAE
                     GENLKEVGCI"
BASE COUNT          758 a          451 c          682 g          599 t
ORIGIN      
        1 agattgcggt acaggcaacg gtgttgtgat gaaagcactg gtagaaggtg gtgagattat
       61 tgaagcattg cgtgaacgga ttcttggcag agtcgtagcg atcgatgtag taaatcctga
      121 aaatcaggag atcgtttttg ctgcaggcga attgttagat gaagatgcag ttgatttaat
      181 tgaatcatta ggtatcgatg aagtaaaagt tcgcacgcca ttgacttgcg agacgcgcta
      241 cggattgtgc gctaagtgtt atggccggga tctggggcgc ggcacgccag ttaacgtcgg
      301 tgaagcagta ggcgttatcg ctgcgcaatc aattggtgag cctggcacgc agttgacgat
      361 gcgcacattc catatcggcg gtgcagcatc gagaacggct gtggttagtc aagtagaaag
      421 taagtcaagc ggtatagtgc attactcatc cacgatgcgt tatgtaacca atgcacaaaa
      481 tgaattaatt gccatatcgc gtagcggtga aatcattatt caagatgaaa atggccgtga
      541 gcgtgaaagg cataaggcat catatggtgc gacattgatg gtgcggaatg gtgaaacggt
      601 taaggcaggt caagtactga ctacttggga tccgcatacg cgaccgatta tcactgagta
      661 tagcggtaag gttcgtttcg aaaatgtcga agaaggtgtg acggttgcca agcaaatcga
      721 tgaagtcact ggattatcga cattggtagt tattgatccg aagcgtcgtg gtgtattgca
      781 atcaaaaggc ttgcgtcctt tggtgaaatt cttggatgac gacggtaatg aaattaaaat
      841 ggtcggaagt aatcagtccg tgagtattac attccagatt ggttgcatca ttactgtacg
      901 tgacggacag caagtaagtg ttggtgaggt attagcacga attccgcaag aaacatccaa
      961 aaccagggat attactggcg gtttgccgcg cgtggctgaa ttgtttgagg cgcgatcacc
     1021 gaaagatgct ggtatgctag ctgaagtaac cggtatcgtg tcgttcggga aagataccaa
     1081 aggtaagcag cggcttgtca tcactgatct ggaaggtatc gttcatgaat atctcatacc
     1141 gaaggataaa catgtcatgg cgcatgatgg gcaagttgtc aacaaaggtg aaattatcgt
     1201 tgacggtcca gctgatccgc gcgacatatt gcgtttacaa ggtgttgaag cactagctcg
     1261 ctatattacc gatgaagttc aagatgtata ccgtttacaa ggtgttagaa taaacgacaa
     1321 acacattgaa gtcatcgtgc gtcagatgtt aaggcgagtg caggttatta atgcaggaga
     1381 ttcgtcattt atacctggcg aacaagttga gcgagctgat ttgttaattg aaaatgagcg
     1441 gctaattgct gagaaaaaaa tgccagctac ctatgagttc ttattgctcg gtattacaaa
     1501 agcatcgcta tcaacagatt ctttcatctc agctgcgtca ttccaggaaa caacgcgagt
     1561 attgactgag gcagccatca tgggtaaaaa agatgatttg cgaggactga aagagaatgt
     1621 tattgttggt aggcttgttc cagcagggac aggattatct tttcatagca aacgaaggaa
     1681 tcaagaaaag atattcgaat ccggtttgga atcagatatt tcaatagata gtacagtcta
     1741 gcaattaaaa gcctattact cgattatcaa atcatccctt gacagttata agagtactaa
     1801 gtagtcttta ctgttgtgtg gctattactg aggctatggc ggatgatcag aggctgaggt
     1861 gtgataaaaa agagtaaggg caattagccg aactctgctg aggttgcacg agagatcaat
     1921 aagaagagag aattttgagg gataggaagg aagaaaatgg aaaaccaaag gagagagaga
     1981 tgaaaggcaa gctatggccg aagatagtag cggcaatgtt aggtggtctg ctgataggag
     2041 cggcacacgc gaacataccg agcgtaccga atgaattata tgaagcattg aagctggatc
     2101 gcgagaaagt aacgccgaag gaactgcacg aagcgctggt taagcgctac aaggatcctg
     2161 cacaaggatc gggacgcggc accctggcgc aatactggga gccgataccg tacggtatat
     2221 atttggatcc caatacattt tacaaatcac cgaccacgaa taaagaagtt gcaagccgta
     2281 aagaatgcgt ggaatgccac accgacgaat caccggtatg ggtacaagca tggaagagaa
     2341 gcagccatgc gaacctggac aaagttcgca acctgaaacc gggcgaccct actttttaca
     2401 agaaagctaa gctggaagac atcgaaaaga acttacgctc gatgggcaga ctggctgaag
     2461 gcgagaacct gaaagaagtc ggctgtatcg
//
LOCUS       DAOQHY010000303         2489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_30158_length_2489_cov_5.133525, whole genome shotgun sequence.
ACCESSION   DAOQHY010000303 DAOQHY010000000
VERSION     DAOQHY010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30158_length_2489_cov_5.133525"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="PLL28_14845"
     CDS             complement(<1..731)
                     /locus_tag="PLL28_14845"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HNF70657.1"
                     /translation="MQHKYSISLVSYLNTKPFLYGLQHEPEAAQFALELDIPSVGGKK
                     ILENQVDIGLVPVAVLAKLDDARIISDYCIGADGPVRSVCLFSHVPIDKIEKVFMDYH
                     SMTSVQLVNILLKEYWHVHPEQIPATTGYIEQIQGTTAGLIIGDRAIGLYDKFPYVYD
                     LSEAWKSHTGLPFVFAAWITRKEISADVLQSLNKALHLGVESIDAVVNMYAGNQLDKH
                     ALHDYFTLNIDYHLDGVKRKALELF"
     gene            complement(721..1332)
                     /locus_tag="PLL28_14850"
     CDS             complement(721..1332)
                     /locus_tag="PLL28_14850"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNF70658.1"
                     /translation="MKKEIYIIRHGETDFNQLGIVQGRGVDSSLNETGRKQAQHFFDT
                     YKDAGFQKIFVSTLKRTHETIAPFRALGIPIIAHKGLDEISWGIHEGKKDGDTFKDFY
                     RVLHAWQHGETDLAIDDGESPITVQQRQLRFLDELTASGEDRILICSHGRAIRILMCT
                     MLKKPLSEMDTFPHQNTSLYKMIYDGNSFATDLFNDITHLHAT"
     gene            complement(1361..>2489)
                     /gene="mqnE"
                     /locus_tag="PLL28_14855"
     CDS             complement(1361..>2489)
                     /gene="mqnE"
                     /locus_tag="PLL28_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HNF70659.1"
                     /translation="ADKVLNGERITPAEGIVLYEKASLGFAGTLADHVRTRMHGNIAY
                     FNRNFHIEPTNLCVFDCKFCSYSRMLKQRSDGWVMSKESMMDIVRSYADKQVTEVHIV
                     GGVLPELNLEWFADVLSSIKAMRPDLHIKGFTAVELEYMIRKAKMSYRDGLAYLQKAG
                     LDSLPGGGAEIFDETIREQICADKCDSATWLEIHRCAHELGMHSNATILYGHIESFAH
                     RIDHMERLRSMQDETKGFNCFIPLKFRNQHNQMSNVEECSVVEDMRNYAIARIYLDNF
                     PHIKAYWPMIGREQAQLSLHYGVNDIDGTIDDTTKIYSMAGAEEQSPVMTTRQICELI
                     LAAGKQPVERDTLYNHIKSYSHESLDAEFGVDGFIGLPVAN"
BASE COUNT          691 a          561 c          472 g          765 t
ORIGIN      
        1 ataaataatt ccaatgcttt tcgttttaca ccatcaaggt ggtaatcgat attaagggta
       61 aaataatcat gcaatgcatg tttatccaat tgattgccgg catacatatt cactactgca
      121 tcaatacttt ccacccctaa atgcaaagct ttattgagtg attgcaatac atcagcagaa
      181 atttcttttc tggtgatcca tgcagcgaat acaaatggca aaccggtatg cgacttccat
      241 gcttcactca gatcatatac ataagggaat ttatcataca acccaattgc gcgatcgcca
      301 atgatcaagc cggcagttgt gccttgaatt tgttcaatat atcctgttgt agccggaatt
      361 tgttcaggat gcacatgcca atattccttc aacaagatat ttactaattg taccgaagtc
      421 atagaatgat aatccatgaa tactttttct atcttatcga taggcacatg actaaacaaa
      481 catacactgc gaacaggtcc atcggcacct atacaataat ctgaaatgat ccgtgcatcg
      541 tcaagttttg ctaataccgc aaccggcacc aatccgatat ctacttgatt ttccaaaatc
      601 ttttttccgc caacagatgg aatatccaat tcaagcgcaa actgtgctgc ctcgggttcg
      661 tgctgtaatc cgtaaagaaa tggcttggta ttcagataac tgactaatga tatactgtat
      721 ttatgttgca tgcaaatgcg taatatcgtt aaacaaatcg gtggcaaagc tattgccatc
      781 gtagatcatt ttatataaac tggtgttttg atgcggaaag gtatccattt ccgataaagg
      841 tttcttcagc attgtgcaca tcagaatgcg aatagcgcga ccatgactgc aaatcaggat
      901 gcgatcctcc ccgcttgctg tcaattcatc taaaaacctt aattgtcgct gctgaacagt
      961 gatcgggctt tcgccatcat caattgccaa atctgtttca ccgtgctgcc aggcatgcaa
     1021 tacgcgataa aagtctttga aggtatcgcc atctttcttg ccttcatgaa ttccccagct
     1081 tatttcatcc agtcctttat gagcaataat cggtatgcca agtgcgcgga atggcgctat
     1141 tgtttcatgt gtgcgcttga gtgtgctgac gaatattttt tgaaaaccgg catccttgta
     1201 tgtatcgaaa aaatgttgcg cttgctttct gcctgtttcg ttgagtgaac tatccactcc
     1261 cctgccctgc acaattccca attgattgaa atctgtttcg ccgtgtcgga tgatatagat
     1321 ttcttttttc aaagtaaaga agcagattta ataattggta ttaattcgca acaggcaatc
     1381 cgataaatcc atcaacacca aattcggcat ccaatgattc atgactgtat gatttaatat
     1441 ggttgtaaag tgtatcgcgt tcgacaggtt gctttcctgc agctaatatc aattcacata
     1501 tctggcgtgt ggtcattacc ggcgattgtt cttcagcgcc tgccatgcta taaattttcg
     1561 ttgtgtcatc aatcgtacca tcgatatcat ttacaccata atgcagactc aattgcgcct
     1621 gttcgcgacc tatcattggc caatatgctt ttatgtgcgg gaaattatcg agatagatac
     1681 gggcaattgc ataattgcgc atgtcttcta caacactaca ttcttcaacg ttgctcatct
     1741 ggttatgctg attgcggaat ttcaaaggaa taaaacaatt gaatcccttt gtttcatcct
     1801 gcatgcttct caatcgctcc atatgatcaa tgcgatgagc aaaactttcg atatgaccat
     1861 atagaattgt tgcattacta tgcataccta attcgtgcgc acaacgatgt atttcgagcc
     1921 aggtggcgct atcacattta tcggcgcaga tctgttcacg tatagtttca tcaaatattt
     1981 ctgcaccacc accgggaagg ctgtctaatc cggctttttg gagataagca agaccatcgc
     2041 gatagctcat ttttgctttg cggatcatgt attccaattc aacggcagta aaacctttga
     2101 tatgcaaatc ggggcgcatt gcttttatgc tgctcagcac atctgcgaac cattcaagat
     2161 ttaattcagg taatacaccg ccaacaatat gcacttctgt aacctgctta tcggcataag
     2221 agcgcacaat gtccatcatg gattctttgc tcatcaccca accatcactg cgttgtttga
     2281 gcattctgga atagctgcaa aatttgcaat cgaacacgca aaggttagtt ggttcgatgt
     2341 gaaagttcct attgaagtag gcgatattgc catgcatacg tgtgcgcaca tggtctgcca
     2401 gagtgcccgc gaagccgagt gaagcttttt catacaatac aataccttct gccggcgtga
     2461 tgcgctcgcc attcaatact ttatctgca
//
LOCUS       DAOQHY010000304         2392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_32270_length_2392_cov_6.798460, whole genome shotgun sequence.
ACCESSION   DAOQHY010000304 DAOQHY010000000
VERSION     DAOQHY010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32270_length_2392_cov_6.798460"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1446)
                     /locus_tag="PLL28_14860"
     CDS             complement(<1..1446)
                     /locus_tag="PLL28_14860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNF70660.1"
                     /translation="MQFKFSILGITLFVSAVVSAQWTNTFNGQGDFSDVFQASVADNA
                     GNTYAAGYTINRDVSKDILLAKFNAAGDTVWTRQYAGTGNGPDEALAMLISTDGNIVI
                     TGYQKGAGTGFDFITQKYSTNGNLLWTSFYNYSTNESDQSNAITSDASGNIYIAGQSD
                     KDVSPVNDDDYVVVKYNADGVQQWAKRSDGAIHSTDRPSAIAIGTDGNVIVTGRSSNG
                     SDDDYLTIKYNSSTGNEMWRKLFDRTHHDRATDIAIDPSTGKIFVTGRSNNGTNYDYA
                     TLCYNTSGVEQWQAIFDYVDDDRATNIALDGSGNVYVAGQSDADASTVTINYNFLLVK
                     YNSSGVQQFAQSWDGTAGDDDIPSNLIVDASGNSTITGYSDSDAGVAINNKIAAVRFN
                     AGGVMQWAKTSTIAGSSSLSTSSAMDASGNIIVAGYAESIPNRDACVVKWNNAGVEQW
                     TSTFNGIGDNGDNAHAIIRDNLNNTIVAGYTT"
     gene            complement(1792..>2392)
                     /locus_tag="PLL28_14865"
     CDS             complement(1792..>2392)
                     /locus_tag="PLL28_14865"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNF70661.1"
                     /translation="ADSMIEAAMGGGLFIDEAYALSQGGENDFGREAIEALLKRMEDR
                     RGDFIVIVAGYPDNMAIFLESNPGLKSRFDKFVHFEDYNAEELFTIADNLLQKEYLFL
                     DAAAGEHLKAYITQLVAARNKYFGNARTMRKLVAEVVHSQNLRMAGIPAAQRTPEMIK
                     TVTLDDVNDFATDIETKSSIREAIGFKFGQDKGDASQTK"
BASE COUNT          630 a          543 c          479 g          740 t
ORIGIN      
        1 tgttgtatat ccggcaacaa tggtattgtt taaattgtcg cgaataatcg catgggcatt
       61 atctccatta tcgccaatgc cattgaaagt tgatgtccat tgttcaacgc cggcattatt
      121 ccattttaca acacatgcat cgcggtttgg aatggattca gcatatcctg caactataat
      181 attgcccgat gcatccatgg ctgaagatgt tgacaaactg ctgcttcctg caatggttga
      241 agtttttgcc cattgcatta ctccaccggc gttaaatctt accgctgcaa ttttattgtt
      301 gattgcaact ccggcatcac tatcgctata tccggttatg gtagaatttc ctgaagcatc
      361 aacaattaaa tttgatggaa tatcatcatc tcctgccgtg ccatcccacg attgcgcaaa
      421 ttgttgaaca cctgagctat tgtattttac aagcaggaaa ttataattta ttgtaaccgt
      481 tgaggcgtca gcatcacttt gaccggctac atatacattt ccggatccgt ctagggcaat
      541 atttgttgcg cgatcatcat ctacataatc aaatatggct tgccattgct ctactcctga
      601 tgtgttgtaa cataaagtgg catagtcgta attcgtgcca ttattactgc gaccggtaac
      661 aaatattttc ccggtacttg gatcaattgc aatatcagta gcacgatcgt ggtgcgtgcg
      721 atcaaataat tttcgccaca tttcattgcc tgtgctgcta ttatatttta tagtaagata
      781 atcgtcatca ctaccattgc tacttctgcc tgtaacaatt acgtttccat ctgtgccaat
      841 tgcaattgct gatggtctgt cggttgaatg aattgcgcca tcggagcgtt ttgcccattg
      901 ctgcacaccg tcggcattgt atttaactac tacataatcg tcatcattta ccggactcac
      961 atctttatcg ctctgaccgg cgatataaat attgcctgat gcatctgaag taatagcatt
     1021 actttgatca gattcgttgg tagaataatt gtagaatgat gtccaaagta agtttccgtt
     1081 ggtgctatac ttttgggtaa tgaaatcaaa tcctgttccg gcgcctttct gatatccggt
     1141 gattacaata ttgccatctg ttgaaataag cattgccaac gcttcgtccg gtccatttcc
     1201 ggtgccggca tattgacgtg tccatacagt atctcctgcc gcattgaatt ttgccagtag
     1261 aatatctttt gaaacatcgc gattaatggt atacccggct gcatatgtat tgccggcatt
     1321 gtcagcaact gaagcctgaa atacatcgct gaagtcgcct tgcccgttga aagtgtttgt
     1381 ccattgtgct gacacaacag ctgatacaaa taaggtaatg cctaagattg aaaatttgaa
     1441 ttgcatgtgg tggattgttc agttccgaaa ccaaaagtat ttcggttcta aaccccactc
     1501 aaggacaaaa agacctcatt ttccctcgat ttttgcgttt ttccgcctgt atttgtcgtg
     1561 ttgacgtgag gggctttcgc ctgaaatgcc tgcaaatagg gcatcttgcc gtattacctc
     1621 gccctatttt ctccgataca aaaatgccct tttcgacaaa tcaagggctg aaaaagggtg
     1681 tttttccatg aaaaaacggc agaagatacc tgtatataca gttaaaaaca tgtctataca
     1741 tatataatat atgtatagat acgtatctca ctccactctg atgacctaag gtcacttggt
     1801 ttgggaggcg tcgcccttgt cctgaccgaa tttgaaaccg atagcctcgc gaattgatga
     1861 ttttgtttca atatccgtcg cgaaatcatt cacatcatca agggtaaccg ttttgatcat
     1921 ctcaggtgtg cgctgagcag caggaatacc tgccatacgc aaattttggg aatgcacaac
     1981 ctcggctacc agtttgcgca tggttcgggc attgccaaaa tatttgtttc gggcagcaac
     2041 taattgggtg atataagctt tcagatgctc tccggcagct gcatctagga acaaatactc
     2101 cttttgaagc aggttatcgg ctatggtgaa tagctcttcg gcgttataat cttcaaaatg
     2161 cacaaacttg tcgaagcgcg attttaatcc gggattggat tccaggaaaa tagccatatt
     2221 atcgggatat ccggcaacaa tgacgatgaa gtctccgcga cggtcttcca tacgctttaa
     2281 taaagcttct attgcctcgc gaccgaagtc attctcaccg ccctgactca aagcataggc
     2341 ctcatcaata aataaaccac cgcccatcgc tgcttcgatc atgctatctg ct
//
LOCUS       DAOQHY010000305         2358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_33058_length_2358_cov_5.851932, whole genome shotgun sequence.
ACCESSION   DAOQHY010000305 DAOQHY010000000
VERSION     DAOQHY010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33058_length_2358_cov_5.851932"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PLL28_14870"
     CDS             complement(<1..184)
                     /locus_tag="PLL28_14870"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNF70662.1"
                     /translation="MQFKQYINGNWCDAANAGTQQVIDPANGEYVQTVPYGDGSDTTI
                     AIDAAHDAFRTWKKMSP"
     gene            complement(193..1512)
                     /locus_tag="PLL28_14875"
     CDS             complement(193..1512)
                     /locus_tag="PLL28_14875"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNF70663.1"
                     /translation="MSTEVVMNKTASLYERRKQLVPNALGIFNPSSISHASGAIVYDA
                     DGRALIDFAGGIGVLNAGHCPEPVVRAIQQQAEKFIHACFNVSIYEQYLDAAQQMIDI
                     LPHGDATKIMFTNSGAEAIENAIKIARQATGRSAVIAYTQAFHGRTLMAATLTSKVGY
                     KTGFGPYAPEIYRIDFPAFAAYEGPLDEDSFSDLHIENLRSFFNTHIPAKQVAAIILE
                     PVQGEGGFHVVPKHYLQQLRNLCNEHGIVLILDEVQTGFGRTGSWGAYQQLGVIPDIS
                     TWAKSMGSGMPVGAVMGKAHIMDAAIAGTIGGTYLGNPLCMVAVSATINYMKEIDINA
                     KGMHVGSIIRKRFMQLRDNYPQHISSVRGMGAMMAFELSYDGDIRKPNGDLCKKITQR
                     CYENGVIAISSGIHGNVIRNLAPLVITDEQLESGLDILEEAVAYYCK"
     gene            complement(1509..>2358)
                     /locus_tag="PLL28_14880"
     CDS             complement(1509..>2358)
                     /locus_tag="PLL28_14880"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNF70664.1"
                     /translation="FLDAGFPEHVFQTVVIDVDLVENIIAHDIVQGVTLTGSERAGSA
                     VASLAGKHLKKTVLELGGSDALIVLNDADVNLAAKVALQSRFQNAGQSCIASKRFIVE
                     EKIRPDFIQALINGISAMKQGDPFNADTTMGPMARMDLAAQLESQLQISLRKGAGLLA
                     GGARKGANFLPSVLDHVGKGMPAFDEELFGPIAAVISAANADEAIAMANDSRYGLGAS
                     IFTQDIDKAKHYARQIESGSVFVNALVKSDPRIPFGGIKRSGYGRELSHFGMKEFMNI
                     KTIYIA"
BASE COUNT          656 a          576 c          442 g          684 t
ORIGIN      
        1 atggcgacat tttcttccat gttctaaatg catcatgtgc cgcatcaata gcaattgtag
       61 tatcactgcc atcgccgtaa ggcacagttt gcacatactc accattagcg ggatctatta
      121 cttgttgcgt gcctgcattt gcagcatcac accaattccc atttatatat tgtttaaact
      181 gcatgcgtga aattatttac aataataggc aacagcttct tccaaaatat ctaatccact
      241 ctcgagttgt tcatcagtaa ttaccagagg ggcgaggttg cggatcacat ttccatgtat
      301 gccggagctg attgcaatca caccattttc gtaacagcgt tgcgtgattt ttttacagag
      361 atcaccattg ggtttgcgga tatcgccatc atacgataat tcgaatgcca tcatggcacc
      421 cattccacgc acactggaaa tatgttgcgg ataattatca cgcaattgca taaatcgctt
      481 tctgatgatg ctgcccacgt gcatgccttt tgcattaata tcgatttcct tcatataatt
      541 tatggtagcg cttacggcaa ccatgcataa aggattaccc aggtaagtac caccaattgt
      601 tccggcaatt gcggcatcca tgatatgggc ttttcccatc acagcaccaa ccggcattcc
      661 acttcccata cttttcgccc aggttgagat atctggtatt acacccaatt gttgatacgc
      721 gccccaagaa cctgtgcgac caaaacctgt ttgcacctca tccaatatga gcacaatacc
      781 atgttcattg cagaggtttc ttagctgctg taaataatgt ttcggcacca catgaaatcc
      841 accttcacct tgaacaggtt caagaataat ggcagccact tgtttagcag gaatatgcgt
      901 attaaaaaac gagcgcaaat tttcgatatg taaatcgctg aatgaatctt catccaatgg
      961 cccctcatat gctgcaaatg ccggaaaatc aatgcgataa atttcaggcg cataaggacc
     1021 aaatccggtt ttataaccca ccttacttgt gagtgtggca gccattagcg ttcttccatg
     1081 aaatgcctgg gtataagcaa tcactgcaga tcttcctgta gcctgacgtg cgattttaat
     1141 tgcattctca attgcttccg caccactgtt tgtaaacatg atttttgtgg catcgccatg
     1201 aggcaatatg tcgatcattt gttgagccgc atcgagatat tgctcataaa tggaaacatt
     1261 aaaacatgca tgaataaatt tctctgcctg ctgctgaata gcacgcacaa caggttccgg
     1321 acaatggccg gcattcaaaa caccgatacc tcctgcaaag tcgatcaacg ctcgaccatc
     1381 tgcatcataa acaatggcgc ctgaagcatg ggaaatactg gatggattaa aaatccccaa
     1441 tgcattcggc actaattgct ttcttcgctc atacagtgat gctgttttat tcattactac
     1501 ttcagtactc atgcaatata aatggtttta atattcataa attctttcat tccgaaatgt
     1561 gataattctc gaccatatcc agatcgctta ataccaccaa aaggaatacg tggatctgat
     1621 ttcaccaatg catttacaaa cacacttccc gattcaattt gtcgcgcgta atgttttgct
     1681 ttatcaatat cttgagtgaa aatcgaggcc cctagtccat aacgggaatc atttgccatg
     1741 gctattgctt catcggcatt agctgcggaa atcacagctg caattggacc gaacaattct
     1801 tcatcaaatg cgggcattcc ttttcccaca tgatcgagca ctgaaggcaa aaaatttgct
     1861 cctttgcgcg cgccgcctgc aagcagtccc gcaccttttc gcaaagaaat ctgcaattgc
     1921 gactccaatt gtgccgccag atccatgcgt gccataggcc ccatggttgt atcggcattg
     1981 aaaggatcac cttgtttcat agcggaaatt ccattgatga gtgcttgaat aaaatcgggt
     2041 ctgatttttt cctccacaat aaaacgcttt gacgcaatgc aactttgacc tgcattttgg
     2101 aacctgctct gcaatgccac cttagcagcc agatttacat cggcatcatt taatacaata
     2161 agcgcatcgc ttccgccgag ttcgagaaca gtttttttaa gatgttttcc tgccaacgaa
     2221 gcaactgcac ttccggctcg ttcgctacct gttaaggtaa cgccttgaac gatgtcatgt
     2281 gctatgatgt tttctacaag atcaacatct atcaccactg tttgaaaaac atgttcagga
     2341 aatccggcgt caagaaat
//
LOCUS       DAOQHY010000306         2338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_33560_length_2338_cov_4.475252, whole genome shotgun sequence.
ACCESSION   DAOQHY010000306 DAOQHY010000000
VERSION     DAOQHY010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33560_length_2338_cov_4.475252"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PLL28_14885"
     CDS             complement(<1..615)
                     /locus_tag="PLL28_14885"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HNF70665.1"
                     /translation="MQALIEKAWNDRSLLQDTAMQHTIKEVIAMLNNGSLRVAEKVNG
                     NWQVHEWVKKAVIMYFPIMQMETISVGPFEFHDKIPLKHNLKEAGIRVVPHGLIRYGA
                     FVARNVIVMPGYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEPVQA
                     SPVIIEDNCFIGSRCIVVEGVIVEEEAVLGANVVLTQSTKIIDVT"
     gene            741..2222
                     /locus_tag="PLL28_14890"
     CDS             741..2222
                     /locus_tag="PLL28_14890"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNF70666.1"
                     /translation="MRAKLIMLLGPVLAVITGLLFYYTGNSPEVSKMAAVLVLMAYWW
                     MTEAVNIFITSLLPVVLLPLLGILSIDAVAPQYMKDVIFLYIGGFILTFAIERWNLHK
                     RFSLGILLRFGSSPEKLLIGFMVSTYFISMWLNNTSTTLMMLPVALAVIKQIHGNDDT
                     KRTGLSTAILLGISYASTIGGTATLVGTLPNMVMKSFYDEHFPAEQSLNFANWFSFAF
                     PMSLFFLAICYFVFKAMLLRNSDHKAPDLEYCKTEYRALGKMTFEEKAIAWIFLAAVV
                     LWFTLDNKTLWGLDLVGWKTYLVHWHIIPNDKFVNESFVAILMGMILFFFPSKSKKGE
                     SLVMWEDVKRLPLGILFLFGGGFALAKGVEVSGLDDWIAGNCTGLKDLPPILMILCLC
                     VITTIFSEFASNTATVSLFMAILIPIIPMLGIPPLLAMMPITIAASYSFMLPVGTPPN
                     TIVFGTEMIKVRTMLRVGLWLDLLGALVILLFTFTIGQWVFGF"
BASE COUNT          596 a          504 c          488 g          750 t
ORIGIN      
        1 cgttacgtca atgatttttg ttgattgcgt taataccaca ttcgctccca acacagcttc
       61 ttcttccaca ataacccctt ccactactat gcatcgcgat ccgatgaagc aattatcttc
      121 aataattacc ggacttgcct gcacgggttc aagcacgcca ccaatgccaa caccgccgct
      181 gagatgcaca tttttgccaa tttgggcaca actgcctaca gtagcccatg tatcaaccat
      241 ggtgccttca tcaacatacg caccgatatt tacgtatccg ggcatcacga ttacattgcg
      301 tgctacaaag gcaccatagc gaatgagacc atgtggcaca acgcggattc ctgcttcttt
      361 gaggttatgt ttcagcggaa ttttatcatg aaattcaaaa ggtcctacgc ttatggtttc
      421 catttgcatg ataggaaaat acatgatcac cgcctttttt acccattcat gcacctgcca
      481 gttgccattc accttttccg caacgcgtaa actgccgttg ttcaacatgg caattacttc
      541 cttaatggta tgctgcatgg ctgtatcctg caaaaggctg cggtcgttcc atgctttttc
      601 tattaatgct tgcatgctgc aaatttaggg atattgcatt tagtagtccc gatccgggca
      661 atatctctgt aattgtcgcc ttaaaaggta tgtgcggatg tgcttatatt tgacgttgca
      721 acaattcagc tttccacccg atgcgcgcaa aattaatcat gttattagga cctgtccttg
      781 cagtaatcac cggcctgctc ttttattata cgggcaattc gcctgaggtt agtaaaatgg
      841 ctgctgttct ggttttgatg gcctattggt ggatgacgga agccgtcaat atatttatta
      901 ccagtttatt gcccgtagtg ttattgccat tgttaggcat cctgagtatc gacgcggttg
      961 caccgcaata tatgaaggat gtgatatttc tctatatcgg aggatttatt ctcactttcg
     1021 cgattgagcg ctggaattta cacaagcgat tttcattagg tattttgctt cgctttggca
     1081 gttcgccgga aaaattactc atcggtttta tggttagcac ctattttatt tcaatgtggc
     1141 tgaacaatac ttccactaca ttgatgatgc tgcctgtagc cttggcagtg atcaaacaaa
     1201 ttcatggcaa tgacgatacc aaacgcaccg gattgtcaac ggcaatatta ttggggattt
     1261 cttacgcatc caccattggc ggtacagcaa cattggtagg caccttgcca aatatggtta
     1321 tgaaatcatt ttatgatgaa catttccctg ccgaacaatc cttgaatttc gcaaactggt
     1381 tctctttcgc attcccaatg agtttgtttt ttctggcgat atgctatttt gtttttaagg
     1441 caatgctcct tcgtaattcc gatcacaaag ctccggatct ggaatattgc aaaacagaat
     1501 atcgtgcact cggaaaaatg actttcgaag aaaaagcgat tgcatggatt tttttggctg
     1561 ctgtagtatt gtggtttaca ctcgacaata aaaccttatg gggtctcgat ctggtaggat
     1621 ggaaaactta tctagtacat tggcatatca ttccaaatga taagttcgtg aatgaaagct
     1681 ttgtcgcaat attaatgggc atgatattat ttttcttccc gtcaaaatct aaaaaaggcg
     1741 agtcgttggt aatgtgggaa gatgtgaagc ggttgccact tggtatatta tttttattcg
     1801 gcggaggatt cgcacttgca aaaggtgttg aagtgtcggg actggacgat tggattgcag
     1861 gtaattgcac cgggttaaaa gaccttccac ccattttaat gatcttatgc ctttgtgtta
     1921 tcactaccat attttctgaa tttgcttcga atacggcaac cgtgagtttg tttatggcca
     1981 ttttaattcc cattatcccg atgcttggaa taccgccatt gctcgcgatg atgcctataa
     2041 ccattgctgc cagctattca ttcatgctgc ccgttggaac cccacctaat acgatagttt
     2101 tcggaacgga aatgattaaa gtgcgcacca tgctccgggt tggtttatgg ctcgatttac
     2161 ttggtgcatt ggtcatcctg ttgttcacct ttaccattgg gcaatgggtg tttggattct
     2221 gaaagacatt gctataagaa atcaattaat gccaatgttg acaggcaaaa aaaaagacca
     2281 tcaacagatg atcttttttt gggtttacgc tacaattgtt tggtcaatgc gttacggt
//
LOCUS       DAOQHY010000307         2333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_33688_length_2333_cov_4.763389, whole genome shotgun sequence.
ACCESSION   DAOQHY010000307 DAOQHY010000000
VERSION     DAOQHY010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33688_length_2333_cov_4.763389"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="PLL28_14895"
     CDS             <1..1220
                     /locus_tag="PLL28_14895"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNF70667.1"
                     /translation="YGGADLYMSKRNAEGWDAAVNVGQSLNTKGNEVFPSFDAAGTFY
                     FSSDNLQGMGGLDIFSAQWKNGKFSNIQNLEYPINSGADDFGLVMLPSTAYGKDTLAA
                     GYFSSNRAGGLGNDDLYLFVKTKKKLPPPLYVLNGKVSRKVYEIENDVNSKVIDTIPL
                     DAATAQLFYPEQKKELAMFTVSESGMFTLFVDSTQQYKIVGSKEGYFNNSTLVDITKQ
                     KGKPGDTVKVFAEVVLDMIPIATGTTTAEIRLENIYYDYDSPNLRPESFPELDKLITL
                     LNENPGMTIQINSHTDARGKDAYNEKLSAGRAQSVVNYLVEKGIAVERLTAKGYGEKS
                     PSTVNKDLTLPSGKIVPKGTLLEEKFINTFKANKYDFEYLHQLNRRTTFNVTGMMNIQ
                     SEDAGDIRIDQAD"
     gene            complement(1536..>2333)
                     /locus_tag="PLL28_14900"
     CDS             complement(1536..>2333)
                     /locus_tag="PLL28_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNF70668.1"
                     /translation="IPDLHRTLIIWGGLVFFYLIEYAIPLIRFQYKKIQHAGVNIFFT
                     ITTILVNVSLAFILLKASDYVSDHSIGLLYIFSLSPWVFFIAGLLLMDLIGAYTVHYV
                     EHKVKWMWKFHLIHHADTYVDTTTANRHHPGESIFRFVFTTMAVIITGAPMWIVMVYQ
                     SLSVVLSQFNHANIKLPDWLDKGLSWLIVSPNMHKVHHHYMQPYTDSNYGNIFSFWDR
                     VFGTFKHLQPKQIVYGIDTHMGVEENSKIGNLLQIPFQPYRKPGGKL"
BASE COUNT          774 a          486 c          455 g          618 t
ORIGIN      
        1 gctacggagg tgctgatctt tatatgagca aacgcaatgc cgagggatgg gatgctgccg
       61 taaatgtcgg tcaatcactg aataccaagg gcaatgaagt atttccatcc tttgatgctg
      121 caggtacatt ctatttcagc agcgataatt tacaggggat gggaggattg gatatttttt
      181 ctgcacaatg gaagaatggc aaattctcaa acattcaaaa ccttgaatac cctattaatt
      241 caggagccga tgattttggt ttggtgatgt tgccttcaac tgcctatggg aaagatacgc
      301 tggcagcagg atatttctcg tcgaatcgcg caggcggatt aggtaatgat gatttatatc
      361 ttttcgtaaa aaccaagaaa aaattgcctc caccattgta tgtattaaat ggcaaggttt
      421 ctcgaaaagt gtatgaaata gaaaatgatg taaacagtaa agtgattgat accatcccgt
      481 tagatgcggc gacagcacaa ttattttatc ctgaacagaa aaaagaactt gctatgttca
      541 cagtaagtga aagtggcatg ttcacattat ttgtcgacag cacacaacaa tataaaatcg
      601 ttggttcaaa agaaggctat tttaataata gtacactggt tgatataact aaacaaaaag
      661 gcaaaccggg cgataccgtg aaggtatttg cagaagtggt tttagacatg ataccaatcg
      721 caaccggcac tacaacagct gaaattagat tggaaaatat ttattacgat tatgactcac
      781 caaatctgcg acctgaatca ttccctgaac tggataagct catcacctta ttgaacgaaa
      841 accccggcat gaccattcag attaattcgc atacagatgc ccgaggtaag gatgcctaca
      901 acgaaaaatt gtcggcaggc cgcgcacaaa gtgtggtaaa ttatctggta gaaaaaggca
      961 ttgctgtaga acgactcacc gctaaaggat atggtgaaaa atcgccaagc actgtaaata
     1021 aagatctcac tttaccaagt ggcaaaattg ttccgaaagg cacattgctg gaagaaaaat
     1081 tcatcaatac atttaaggct aacaagtacg attttgaata tctgcatcaa ttaaaccgcc
     1141 gcaccacgtt caatgtgaca ggcatgatga atattcaatc tgaagacgcc ggcgatattc
     1201 gcattgatca agctgattag ttttggtagt gagtagtggg tagtgggtag tgagactatc
     1261 ccaacctttg cgcctttgcc tcttcgcggt cttcgcgcga aaccccgcct caccccccgc
     1321 cttctccatc tcctaccgtc gatagagagg gagctgatgg cgcttcggaa ttaaaaattt
     1381 ttcaaaataa tataattgct ttcactatta acgcgccagc gtaaatagga tgccacactt
     1441 gctaacttgc aaactcccaa acttgctcac taaaactaac atctccatgc aagaacaccc
     1501 tcatttgcta atcagctaat ttgctaattc tcaaattaca atttcccacc cggcttgcga
     1561 tatggctgga acgggatctg cagaagatta cctattttac tattctcctc aacacccata
     1621 tgtgtatcaa taccatacac gatctgctta ggctgaagat gtttgaaggt accgaaaaca
     1681 cgatcccaaa aagaaaatat attcccataa ttagaatccg tatacggctg catataatga
     1741 tgatgcacct tatgcatatt cggtgacaca atcaaccaac tcaaaccttt atccagccag
     1801 tcaggcaatt tgatattcgc atgattgaat tgcgataaga ctaccgataa tgactgatac
     1861 accatcacga tccacattgg tgcaccagtg atgataacag ccatggtggt aaatacaaat
     1921 cgaaaaatac tctcccccgg atggtggcga ttagccgttg ttgtatcaac atacgtgtcg
     1981 gcatggtgga tgagatgaaa tttccacatc cattttactt tatgctccac ataatgcacc
     2041 gtgtaagcac caataagatc catcagtaat aatccggcga taaaaaacac ccatggcgat
     2101 aatgaaaata tgtaaagtaa tcctatcgaa tgatcagaaa cataatctga agcctttaat
     2161 aaaataaatg ccaaagaaac atttacaagt atagtagtaa tcgtaaaaaa aatatttaca
     2221 cctgcgtgtt gtatcttttt atactgaaat cgaatcagtg gaatagcata ctcgataaga
     2281 taaaaaaaca caagtccgcc ccaaatgatc aatgtcctgt gcagatcagg aat
//
LOCUS       DAOQHY010000308         2332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_33717_length_2332_cov_3.779534, whole genome shotgun sequence.
ACCESSION   DAOQHY010000308 DAOQHY010000000
VERSION     DAOQHY010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33717_length_2332_cov_3.779534"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PLL28_14905"
     CDS             <1..184
                     /locus_tag="PLL28_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HNF70669.1"
                     /translation="GRSGAYEEVLREAKDIALREMSEKASSMGAHAIIGIDLDYETIR
                     NGMLMVSASGTAVKLA"
     gene            complement(199..588)
                     /locus_tag="PLL28_14910"
     CDS             complement(199..588)
                     /locus_tag="PLL28_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNF70670.1"
                     /translation="MASLQERIDMSVTRTVKAIFPNTTNHYDTLFGGTALMWMDELAF
                     ITATRFSRQRMVTVSSDRVDFKNPVPAGTIAELIGRVTHVGTTSIIIKVEVYLEQMYS
                     DEREKAMEGTFTMVAIDEHKKPVKISY"
     gene            648..1421
                     /locus_tag="PLL28_14915"
     CDS             648..1421
                     /locus_tag="PLL28_14915"
                     /inference="COORDINATES: protein motif:HMM:NF016658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HNF70671.1"
                     /translation="MNVNVAIMIIVTMLSMLLGLPANDNCAMQDNTSLIIADTTIAPT
                     ESFFAAAQSFLSEYVVSGKVNYASIKSDPGELNDLYSMIGTTDLSTADNNTKTAFYLN
                     AYNLIVIHSVLEKYPIASPLDVVGFFDKKKHLVAGQQMTLNELEEKKLRPDPRVHFAL
                     VCAAKGCPKLQSQAYLPATVQKQLVDATTRAVNDPQFLRVDYTNKTVQLSKIFEWYAD
                     DFNKTSGSVIQFINTYRSAKISDSYTLSYYEYDWSLNKL"
     gene            complement(1432..1959)
                     /locus_tag="PLL28_14920"
     CDS             complement(1432..1959)
                     /locus_tag="PLL28_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70672.1"
                     /translation="METLGVGSRIKHPEHGMGVIIHVHKRAYDVCYIDKGIKAMLRDT
                     HVEVIEAMPSEYDVSFTEAEDALIKILREWSDVTEVIHIAEKWEGGTLLMQPKDKSMQ
                     GKEVPIETFFHKIVMLRDRLRVLEQKVNAHNKLTDSEKVEMQQYITRIYGSLTTFNVL
                     FKLKADYFVGDKKEE"
     gene            complement(2091..>2332)
                     /locus_tag="PLL28_14925"
     CDS             complement(2091..>2332)
                     /locus_tag="PLL28_14925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70673.1"
                     /translation="VFPNPVGDVMNFKFSITAQTEMHMQVLDMSMKKKIQKVETVTNG
                     TRTYSLDVRDLPKGSYIFLLRFGNLPPHLCQFEKL"
BASE COUNT          664 a          499 c          472 g          697 t
ORIGIN      
        1 tggccgctca ggcgcttatg aagaagtgct tcgcgaagca aaagatattg cgctcaggga
       61 aatgtctgaa aaagcttcgt caatgggtgc tcatgccatc ataggcatag acctggatta
      121 tgaaaccatt cgcaatggaa tgctgatggt atcagccagt ggtacggcag taaaacttgc
      181 ctgataccgg accttagctc aatagctgat tttaaccggt ttcttgtgct catcaatggc
      241 caccatggtg aaggtgcctt ccatggcttt ttcacgttca tcgctataca tttgttcgag
      301 gtacacctcc actttaataa tgatggaggt ggtgcctaca tgtgttactc tgcctatcaa
      361 ttctgcaatg gtacccgcgg gaacagggtt cttgaaatct accctatcgc tggaaacagt
      421 aaccatacgc tgtctgctga acctggttgc tgtgataaac gcgagttcat ccatccacat
      481 caatgccgtt ccaccaaata aggtatcata atggttggta gtattcggaa aaattgcttt
      541 tacagtgcgt gttaccgaca tatcaatgcg ttcctgtagt gatgccatgg cctgattttt
      601 gattataaga tgcaaaaata cggaggaagg agaacctttg ccgatatttg aacgtaaatg
      661 tcgccattat gatcatcgtt accatgttaa gtatgctgct tggattgcct gcgaatgaca
      721 attgcgctat gcaggataat acatcactta tcattgcaga tactaccatt gcgccgacag
      781 agtcattttt tgccgccgcg caatcattcc tttcggaata tgtggtgtcg gggaaagtaa
      841 attatgcttc cataaaatca gatccgggag aattaaatga cttgtattca atgattggca
      901 caacagatct ttcaactgcc gataacaata ctaaaactgc attttatcta aatgcctata
      961 atctaatcgt cattcacagt gtgcttgaaa aatatccgat agcatcgcca ttagatgttg
     1021 tcggattttt tgataaaaag aagcatttgg ttgcgggaca acaaatgacc ttgaatgaac
     1081 tggaagaaaa gaaattaaga ccggatcctc gtgtacactt tgcattagta tgtgcagcaa
     1141 aaggttgtcc gaaattacaa tcgcaagcct atttaccggc aactgttcaa aagcaactgg
     1201 tagatgctac aacgcgggct gtaaatgatc cgcagttttt aagagtagat tatactaata
     1261 aaaccgttca actctcaaaa atcttcgaat ggtatgctga tgatttcaat aaaacttcag
     1321 gcagtgtgat acagtttatc aatacctatc gcagtgcaaa aatttccgac agttatacat
     1381 tgtcgtacta cgaatacgac tggagtttga ataagctcta gcacttattt tctattcctc
     1441 tttcttatcg ccgacaaagt aatcagcctt taatttaaac aacacattaa aggtggtgag
     1501 cgaaccgtag atgcgagtga tatattgttg catttctact ttctcgctat cagtgagttt
     1561 attgtgtgca tttacttttt gctccagcac gcgcagtctg tcgcgcagca tcacaatttt
     1621 atggaagaag gtttctatag gcacttcttt tccttgcatg gatttatcct taggctgcat
     1681 caggagtgtg ccgccttccc atttttcggc gatatgaatt acttcagtaa catcgctcca
     1741 ttcgcgtaat attttaatca gcgcatcttc cgcttccgta aagctgacat catattcaga
     1801 aggcattgct tcgatgactt cgacatgagt atcgcgaagc attgctttaa tccctttatc
     1861 gatatagcaa acatcatatg cgcgcttatg cacgtgaatg ataacgccca tgccatgttc
     1921 aggatgtttt attctactgc cgactccaag tgtttccata taccattcat ttttcgcaat
     1981 aatactgcat agcaggcaga tttatatatt tctgtagaca caaaaaaaag cccgtttgcc
     2041 acgacagcaa acgggtatcg gtgccgaaca cgacttcagc acttcacaca ctacaacttt
     2101 tcaaattggc aaagatgcgg aggcaaattg ccaaatctca ggaggaatat ataagaacct
     2161 ttgggcaggt cacgcacgtc taaagaatat gttcttgttc cgttagtgac agtttccact
     2221 ttttgaattt ttttcttcat cgacatatcc aaaacctgca tgtgcatttc agtttgagca
     2281 gtaatgctga acttgaagtt catcacatcg ccaaccggat tggggaacac ac
//
LOCUS       DAOQHY010000309         2321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_33998_length_2321_cov_7.037070, whole genome shotgun sequence.
ACCESSION   DAOQHY010000309 DAOQHY010000000
VERSION     DAOQHY010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33998_length_2321_cov_7.037070"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2321)
                     /locus_tag="PLL28_14930"
     CDS             complement(<1..>2321)
                     /locus_tag="PLL28_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70674.1"
                     /translation="FGRIDIITVSSIPKPINHDLYGINIEGLFKQHTLPYDGLNGGSS
                     EYAYDWLAELAPRVIRFPSGESSKFMHLMHNTNGTNSVGYGYDIKEIARYFDQTGGDK
                     TLAAAAAEDGITVNEERLVEDPEEMEDWMDVQFTGNYLDFLLKWKEQQCVDHAYIEDF
                     INLIHTIDAANPGRPKVHVILTLNVISETASECLAIADYLRANNVNVIGVEMGNETYA
                     DFYCKSVGFSDFTHYYDYLNGNNYTGGDYTTTESENLDKVLSDAMWYGGHDYLNTFQN
                     IASYNYKVGLVGKPIGGEFAFKAEDGGGGGTACSDDNWNYLLSGKYDETISGKYAFDA
                     VIMHTYYSADNWDHFVYDIYNFVLSNLTPCNPSPYPSIWDDLWHFGYADPDVRLQTMF
                     EKLRGNGYEEGETGNYMDFLSNHATTPVDLYKYSMLSSFKEFGNQLDLDRTDAGKKDL
                     WVTEWNNKTVITEHTTDHWPLDWPVGIDITNDLQQKMLEAPSNTFLSGYLLMRWWLKE
                     IEMNYYINDFRQNFFTISTMQGYGGGVWSDLLTLTTGDEREAEGTDIVPWEDIGGTIP
                     ETPGCDYIGESGKKFLERNYWMRRMNYYVARLFSDISRNHLLFVPSAFLYDEHNINLP
                     PMVFIDDDVNPQYIYVYFTNVKDVIQNYTLDPQFIRLMFTPDADDVILGIPQINYIIA
                     NELYATFGNTKLYNTSLNNCYDDYEQPTYLANAQMVWGDSYPPSCIGAIHPDHCFTAL
                     PYTIGYFKIPVYPYFEKEGQIQSRLEQDILIYP"
BASE COUNT          697 a          501 c          395 g          728 t
ORIGIN      
        1 tggataaata aggatgtctt gttccaaacg actttgtatt tgcccctcct tttcaaaata
       61 aggataaaca ggaattttaa aataaccgat agtataagga agcgctgtaa agcaatgatc
      121 cgggtgaata gctcctatac atgatggagg ataagaatca ccccaaacca tttgtgcgtt
      181 agctaggtat gtaggctgtt cgtaatcatc atagcaattg tttaaactgg tattatacaa
      241 tttagtatta ccaaatgttg catacaattc attggcaatg atataattta tttgaggaat
      301 acccaaaatt acatcatctg cgtcgggagt aaacataagt cgtataaatt gcggatccaa
      361 agtataattc tgtatcacat cctttacatt agtgaaataa acataaatat attgaggatt
      421 tacatcatca tcaataaaca ccatcggcgg aagattaata ttgtgttcgt catacaggaa
      481 ggccgatggc acaaatagca aatggtttct cgaaatgtca ctaaatagtc tggcaacata
      541 ataattcatt ctgcgcatcc agtaatttct ttcaaggaat tttttacctg attctccaat
      601 gtagtcacaa cctggtgttt caggaatagt gccgcctata tcttcccagg gaacaatatc
      661 agttccctct gcttcccttt catctccggt agtgagcgtc aataaatcgc tccaaacacc
      721 gcccccataa ccctgcatgg ttgaaatggt gaaaaaattt tgtctaaaat cgttgatata
      781 ataattcatt tctatttcct tcaaccacca acgcataagt aaatacccac ttaaaaatgt
      841 attagaaggc gcttccagca ttttttgttg cagatcattc gtaatgtcaa tgcctaccgg
      901 ccaatctagc ggccaatggt cggtggtgtg ctccgttatc acagtcttat tattccattc
      961 agtaacccaa agatctttct tgccggcatc tgttctgtcc aggtcaagtt ggttgccgaa
     1021 ctctttaaag gagcttagca ttgaatattt atacaaatct accggtgtag ttgcatgatt
     1081 tgataaaaaa tccatgtaat ttccggtttc accttcttca tatccattcc ccctaagttt
     1141 ttcaaacatt gtttgaagcc gcacatcggg atctgcatat ccaaaatgcc aaaggtcatc
     1201 ccaaatactt gggtaaggac ttggattgca cggcgtcaag ttgctcagaa caaagttata
     1261 tatgtcatac acaaaatggt cccagttatc agctgaataa tatgtgtgca ttattactgc
     1321 atcaaatgca tattttccgc taattgtttc atcatactta ccggataaaa ggtaattcca
     1381 attatcatcg ctgcatgcgg tgccaccgcc acccccgtct tcagctttaa acgcaaactc
     1441 accaccaatg ggtttgccaa ctaatcccac tttgtagttg tatgacgcaa tgttttgaaa
     1501 tgtatttaag taatcatgtc caccgtacca catggcatca gataatacct tgtctaaatt
     1561 ttcagattct gttgtggtgt agtcaccacc tgtgtaatta tttccattta aataatcata
     1621 gtaatgtgta aaatcgctaa aaccaacact tttgcaataa aaatcggcgt atgtttcatt
     1681 acccatttca acgccaatta cattgacatt gttggctctt aaatagtcgg caattgccag
     1741 acactcagaa gccgtctcac ttatcacatt gagtgtcaaa attacatgca ctttaggcct
     1801 gccgggattt gccgcatcta ttgtatgtat caggttgata aaatcttcaa tatatgcgtg
     1861 gtcaacgcat tgttgctctt tccattttag cagaaaatca aggtaatttc cagtaaattg
     1921 cacgtccatc caatcttcca tctcttcagg gtcctcgact agcctttctt cattaaccgt
     1981 aatcccgtct tcagcagcgg ctgcagccaa agttttatca ccccctgttt ggtcaaaata
     2041 tcgagctatt tctttaatat catatccata acctacacta ttagttccgt ttgtgttatg
     2101 cattaagtgc ataaatttac ttgattcgcc acttgggaac cgaataacac ggggagccag
     2161 ttctgcaagc cagtcatagg catactcact tgaaccaccg ttaaggccat catagggcaa
     2221 agtatgctgt ttaaacaatc cttcgatatt aataccatat aaatcatggt taattggctt
     2281 gggtatactt gaaacggtaa taatatcaat tctgccaaaa t
//
LOCUS       DAOQHY010000310         2319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_34046_length_2319_cov_5.199647, whole genome shotgun sequence.
ACCESSION   DAOQHY010000310 DAOQHY010000000
VERSION     DAOQHY010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34046_length_2319_cov_5.199647"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="PLL28_14935"
     CDS             complement(<1..731)
                     /locus_tag="PLL28_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70675.1"
                     /translation="MLLTIGLSVYGQTPISGVINTYYEVTGIDKCNNSITLTATPIGL
                     AVGDNVAMIQMRGVDADADNDNTYGSILNYAKSGVYELFTVQAISFNVVTLNEVIEKL
                     YSIPGRVQLVRIPEYTDVEVTGTVTGLPWSGTVGGVVALIANGTLTLSADIDASGIGF
                     RGAGVVLNTPCLTGSPVGFDGYVTTAGEDKAGKKGEGISENGDDRYARGAPANGGGGG
                     NDRQTGGGGGSSYAPGGGGGQLLFPP"
     gene            861..1391
                     /locus_tag="PLL28_14940"
     CDS             861..1391
                     /locus_tag="PLL28_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70676.1"
                     /translation="MTPISVTKQVENTINLIPSELSHFDEQAFFNRNGDTWSPAEQVK
                     HLELAVKPLILAFRLPSFVLRLLFGKPNRPGRSYDELLTKYHLKLSEGGKASAPYVPK
                     ALNTAAEKSTVINDCLTRHHKMLKAANSYNDTNLDKYLLPHPLLGKITLREMLFFTDF
                     HIRHHLQSIQQLYGNK"
     gene            1378..1860
                     /locus_tag="PLL28_14945"
     CDS             1378..1860
                     /locus_tag="PLL28_14945"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNF70677.1"
                     /translation="MATNEAARIAKLFSDWYDGTPWTDIKMTDILMDITPEIAAAHPI
                     PGANSIWQLVYHCLGWRINVLQKIQGAEFKSPEDNYLSEPQDFSASAWQQLLKDYAQN
                     EKDWQLYLSNINDDILDSGYLPSKTKFTCYEVIHGLLHHDNYHFGQILMLKKILISKQ
                     "
     gene            complement(1864..>2319)
                     /locus_tag="PLL28_14950"
     CDS             complement(1864..>2319)
                     /locus_tag="PLL28_14950"
                     /inference="COORDINATES: protein motif:HMM:NF027968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HNF70678.1"
                     /translation="DCRKPMVWQDMNYQPEQKLSSQEVRTPVDTVEVNTELLDYYRKL
                     GKLRNMFDVLQAGDYQTIIADNKSGVYVFARSNNNLTVYVVLNNSAKEQKVKLNMPGM
                     RYYYQPLESEITKIKKANVALTIPAVSGTVVFPFTLDECVNVTPEMMGK"
BASE COUNT          677 a          563 c          419 g          660 t
ORIGIN      
        1 ggagggaaca acaattgtcc accacctcca ccgggagcat agcttgatcc gccaccaccg
       61 ccggtctggc gatcgtttcc tccaccacca ccgttggccg gcgcaccacg agcataccta
      121 tcgtcgccgt tttcgctgat gccttctcct tttttaccgg ctttgtcttc accggctgtt
      181 gtcacatatc catcaaatcc gactggcgac cctgttaaac acggagtatt aagcaccaca
      241 cccgcgccac ggaatccaat gcctgaagca tctatatctg cacttaaagt aagtgtccca
      301 tttgcgataa gcgctacaac tccccctact gtaccactcc agggaagtcc tgtcacagtt
      361 cctgtaactt cgacatccgt atattccggt atgcgcacca actgcactct accgggaatg
      421 ctgtataact tttcaataac ctcattgagt gtaaccacgt tgaaggaaat agcttgaacc
      481 gtgaaaagct catacacacc gcttttcgca taattcaata tcgagccgta tgtattgtca
      541 ttgtcggcat cagcgtctac accacgcatc tgaatcatgg ccacgttatc acctaccgct
      601 aacccaatag gggttgcagt caatgtaatg gaattattac atttgtcgat acctgtgacc
      661 tcataatagg tgtttataac ccctgaaata ggagtttgtc cgtacacgga cagcccaatt
      721 gtgaggagca cactacaggt taaaatccta aacttcggca tattgcagtg tgtgtggttt
      781 tttacgaaga taagtaatcg ccacaaaatt gcagatattc aacggatttt gggacttaat
      841 tattcacatt taccgggaat atgacaccaa tttcagtcac aaaacaagtc gaaaacacca
      901 taaatctgat accatcagaa ttatcgcact ttgatgaaca ggcatttttt aatcgcaacg
      961 gcgatacctg gagcccggca gaacaagtaa aacaccttga acttgccgta aaacccctga
     1021 ttttggcctt tcgacttccg agttttgttt tgcgtctcct cttcggtaag cccaatcgcc
     1081 ccggaaggag ttatgacgag cttttgacga aatatcacct taagttgagc gaaggcggaa
     1141 aagcatctgc cccatatgtg ccgaaagcac tcaataccgc tgctgaaaaa tcgaccgtaa
     1201 ttaacgattg tcttaccagg catcataaaa tgttgaaagc agctaacagc tataatgata
     1261 ctaacctgga taaatacctc ctccctcatc ctcttttagg aaagatcaca ctcagagaaa
     1321 tgctgttctt taccgacttt catattcgtc accacctgca atcaatacaa caactatatg
     1381 gcaacaaatg aagctgctcg catcgcgaaa ttattttcag attggtatga tggcacaccg
     1441 tggacagata tcaaaatgac agatatcctc atggacatta cacccgaaat agctgccgct
     1501 catccaatac cgggtgcaaa cagtatctgg caattagtat atcattgttt ggggtggcgc
     1561 atcaatgtat tgcagaaaat tcagggtgcc gaattcaaat cgccggagga taattactta
     1621 tccgaaccgc aggatttttc tgcttcggca tggcaacaat tactgaagga ttatgcgcaa
     1681 aatgaaaagg actggcagct ctatttatca aacatcaatg atgacatttt agacagcgga
     1741 tatcttccga gcaaaactaa atttacttgt tatgaagtaa ttcacgggct cttgcatcac
     1801 gataattatc atttcggaca aatacttatg ttgaaaaaaa tattaatttc gaaacagtaa
     1861 taattatttt cccatcattt caggtgtaac atttacacat tcatctagtg taaaaggaaa
     1921 tactacagtc cctgaaactg ccgggattgt tagtgcaaca ttagctttct taatttttgt
     1981 aatttcagat tccaatggtt gataataata tctcatcccc ggcatattca actttacctt
     2041 ttgctccttt gcactattat tcaatacaac ataaacggtt agattattat tacttctcgc
     2101 aaaaacataa acgcccgatt tattatctgc aataatagtt tgataatcgc ctgcctgtag
     2161 cacatcaaac atgtttctca acttgccaag ttttctgtaa tagtctaaca attctgtatt
     2221 tacctcaacc gtatcaaccg gtgttcttac ttcttgcgat gacaattttt gctcgggctg
     2281 atagttcata tcctgccata ccattggctt ccggcaatc
//
LOCUS       DAOQHY010000311         2302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_34491_length_2302_cov_4.244771, whole genome shotgun sequence.
ACCESSION   DAOQHY010000311 DAOQHY010000000
VERSION     DAOQHY010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34491_length_2302_cov_4.244771"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PLL28_14955"
     CDS             <1..530
                     /locus_tag="PLL28_14955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF70679.1"
                     /translation="QVDNLLNMSRLESGMLMPKYDWCDLQELVYTIIQKLEPGYAQHI
                     IVTAEEDLPLFKLDAGLMEQVLYNIIHNAIQYTPETTTITVKLAHDDDGCNITIADDG
                     PGFPKELIHRAFDKFYRMPVSRTGGSGLGLSIVKGFVEAHKGSITLQNVDSGGSCFEI
                     YIPAETSYISNLKNE"
     gene            523..1200
                     /locus_tag="PLL28_14960"
     CDS             523..1200
                     /locus_tag="PLL28_14960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNF70680.1"
                     /translation="MSKAEILIIDDEPQIRKLLEITLESHEYKVWLATTGREGIVMAA
                     NHPPELILLDIGLPDMNGHEVLQDLRKWYNKAIIILSVLDGGEDIVRALDNGATDYLT
                     KPFRTAELLARIRSAIRRNQQEPLATIITIGDLAIDLPGRTVRLNGETVKLTSTEFNM
                     LALMAKNEGRVLTHQFILKEIWGVGYQSETQYLRVFVASLRKKIEQNPNQPTHIITES
                     GVGYRFM"
     gene            1298..>2302
                     /locus_tag="PLL28_14965"
     CDS             1298..>2302
                     /locus_tag="PLL28_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNF70681.1"
                     /translation="MKKVFESAHSRVSAATLLVALGIIYGDIGTSPLYVVKAVVGERP
                     VTEMLVYGAVSCVFWTITFQTTFKYIYLTLRADNRGEGGIFSLYALVRRYGKYLIIPT
                     IIGASALLADGIITPPISVASAVEGLSNINGLDNIPTMPIVIVIISILFIVQRFGTQI
                     VGRMFGPAMLTWFSMLFILGILQVIQHPDIIKALNPYYAYAFLTQYPGGFALLGGVFL
                     ATTGAEALYSDLGHCGKKNIRITWVFVKTCLITNYMGQAAWLMNSRYDSFLGSENPFF
                     MIMPEWFLLPGVLIATMAAIIASQALISGSYTLINEAMNLNFWPRIAVRQPSDTKGQI
                     Y"
BASE COUNT          657 a          450 c          513 g          682 t
ORIGIN      
        1 ggcaggtaga taatttatta aacatgagcc gactggaaag tggtatgctc atgccaaaat
       61 atgattggtg cgatttgcag gaattggtct atacgataat tcagaaactg gaacccggct
      121 atgcacagca tattattgtc actgcagaag aagaccttcc attatttaaa ttagatgccg
      181 ggttgatgga acaggtatta tataatataa tacataatgc tattcaatat actccagaaa
      241 caacaaccat aacagtaaag ctcgcgcatg acgatgatgg ttgcaatatt actattgcag
      301 atgacggtcc gggatttcca aaggaattaa ttcatcgcgc tttcgacaaa ttttatcgca
      361 tgccggtttc ccgcaccggt ggcagtgggt taggattgtc tatcgtaaaa ggatttgttg
      421 aagcacataa aggaagtatt accttgcaaa atgttgattc aggaggctct tgtttcgaaa
      481 tttacattcc tgcggaaact tcatatatct caaatttgaa aaatgagtaa ggctgaaatt
      541 ttgatcatcg atgatgaacc gcagatccga aaactgctcg agatcacact tgaaagtcat
      601 gagtataaag tatggctggc aactacaggt cgcgaaggta ttgtgatggc tgccaaccat
      661 ccgcctgaat taatattgct cgatatcggt ttgcctgata tgaacggtca tgaggtgttg
      721 caggacctca gaaaatggta taataaagcc atcatcattt tatctgtgct agatggcgga
      781 gaggatattg taagagcttt ggacaacggt gcaacagatt atctcaccaa accatttcgc
      841 actgccgaat tattggcacg cattcgatct gccattcgtc gcaaccaaca agaacccctg
      901 gcaacaataa ttacaatagg agatcttgct atcgaccttc cgggtcgcac agtcagattg
      961 aacggcgaaa cggtcaagct tacatccaca gaatttaata tgctggcatt gatggcaaaa
     1021 aatgaaggtc gcgtacttac gcatcagttc attttaaagg agatctgggg agtaggttac
     1081 cagtctgaaa cgcaatacct gcgcgtgttt gtggcttcct tgcgaaagaa aatagagcaa
     1141 aatccaaatc aacctaccca tattatcact gaaagtgggg taggttatcg atttatgtga
     1201 gtcaggcaca tctttatata atctttatat aaaggaagat tcattttact ttttaccaat
     1261 acctcgcatg ccaactttgc gttgagaatt tttgtgcatg aagaaggtat ttgagtcagc
     1321 gcatagtcgc gtatcggcag caactttatt ggtggcattg ggaattatct atggtgatat
     1381 tggaaccagc cctttatatg ttgtaaaagc agttgtagga gagcgaccgg ttactgaaat
     1441 gttggtgtat ggcgctgttt cctgcgtctt ctggaccatc acttttcaaa ccactttcaa
     1501 atatatttat ctgacattgc gagccgataa ccgcggtgaa ggcggtatct tctcgttgta
     1561 tgcacttgta agacgctatg gtaaatattt gatcatccct accattattg gcgcatccgc
     1621 attgttggct gatggcatca tcaccccgcc aatttcagtt gcatccgccg tagaaggtct
     1681 gtcgaatatt aatggacttg ataatattcc tacaatgcca attgtaattg tgattatttc
     1741 cattttattt attgtacaac gatttggcac acagatagta ggaagaatgt tcggtcccgc
     1801 catgcttacc tggttttcga tgttgtttat tcttggaatc ttgcaagtga tacagcatcc
     1861 cgatatcatc aaagccttga atccctatta tgcttatgca ttcttgacgc aatatcccgg
     1921 tggatttgcg ttgttagggg gcgtattcct cgcaacaaca ggtgccgaag cattgtattc
     1981 tgatcttggt cactgtggga agaaaaatat tcgcattacc tgggtatttg taaaaacatg
     2041 tttgatcacc aattatatgg gacaggcggc atggttgatg aacagcaggt atgatagttt
     2101 tttgggaagc gaaaatccgt tcttcatgat catgccggaa tggtttttat taccgggggt
     2161 gctcattgca actatggcag caatcattgc atcacaagct ctcatcagcg gtagttatac
     2221 cttgataaac gaggctatga accttaactt ctggccgcga attgcggtca ggcaaccttc
     2281 ggatactaaa ggtcagattt at
//
LOCUS       DAOQHY010000312         2259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_35619_length_2259_cov_5.317604, whole genome shotgun sequence.
ACCESSION   DAOQHY010000312 DAOQHY010000000
VERSION     DAOQHY010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35619_length_2259_cov_5.317604"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="PLL28_14970"
     CDS             complement(<1..871)
                     /locus_tag="PLL28_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70682.1"
                     /translation="MKTLFTLPVRVVCCAIFCFMHILAVRAQNMVFNGSFELYDSLPN
                     ETGEWMACTGWNNLNGLGTPDYAHIDGGDVTHMPYTFWCGVYPQDGNAAMSFKTYKDF
                     SENFREYIGTPLIAPLDTTQWYAVSFWITNGDYYHRFGSGTDNIGLLLGTSFRTQPTV
                     HVVDAEPQFVINSMVFNTAWEQHTYYYKPDSAYTCLMMGNFAFDADIATDVFDTAALI
                     FDGAQYFVDNIVIAPIPFVTIIDTGICNGETYILPDGDVAITTLTDTTMLEAIDGSDS
                     LVITHLTVYPTPET"
     gene            1082..>2259
                     /gene="purL"
                     /locus_tag="PLL28_14975"
     CDS             1082..>2259
                     /gene="purL"
                     /locus_tag="PLL28_14975"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HNF70683.1"
                     /translation="MAEALATVEQAKQLGLLPEEFAHITSILGRTPNFTELSIYSVMW
                     SEHCSYKNSIRLLKTLPRSGKMMLVEAGEENAGLIDIGDGLACAFKIESHNHPSAIEP
                     YQGAATGVGGIHRDIFTMGARPIASLNSLRFGNIANTRTQHLMKGVVKGIGDYGNCFG
                     VPTVGGEVYFEDCYNTNILVNAMSVGIVKVGETVSAIAEGAGNPVYIVGSATGKDGIH
                     GASFASGDLHEESHEDLPSVQVGDPFQEKLLLEATLEAIKTGAIVGMQDMGAAGITCS
                     TSEMSAKGKHGMKINLDKVPTRQQNMKGWEILLSESQERMLVIVHKGREKEIEAVFDK
                     WDLHCEIIGEVIEGNTLYYYMHGELVAEVPADSLVLGGGAPIYTRETKEPEYLKDIAK
                     "
BASE COUNT          663 a          453 c          548 g          595 t
ORIGIN      
        1 ttgtttccgg ggtagggtat actgtcagat gtgtgatcac taaagaatcg ctgccgtcta
       61 ttgcctcaag catggttgta tctgtgaggg tagtgattgc cacatcgccg tcaggcaaaa
      121 tatatgtttc cccattacaa ataccggtgt caatgatggt gacaaatgga ataggcgcaa
      181 tcacaatatt atcgacaaaa tattgcgcac cgtcgaatat caatgcagca gtatcgaaaa
      241 catcggtggc aatatcggca tcaaaagcaa aatttcccat catcaaacag gtatatgctg
      301 aatcgggttt gtaataataa gtatgttgct cccatgcggt attaaatacc atactattaa
      361 tcacaaattg cggttctgcg tcaacaacat gcacggtagg ttgcgtgcgg aaagaggtgc
      421 ctaacagcaa tccgatatta tctgttccgg agccgaagcg atgataataa tcgccgttgg
      481 taatccaaaa actcacggca taccattgag tagtatccag tggtgcaata agtggtgtgc
      541 cgatgtattc tctgaaattc tccgagaaat ctttatatgt tttaaaactc atggcggcat
      601 tgccatcttg cggatataca ccgcaccaaa atgtgtaagg catatgtgta acatccccgc
      661 cgtcgatatg tgcataatcg ggcgtgccca aaccattaag gttattccat ccggtgcatg
      721 ccatccattc gccggtttca ttcggtagtg aatcatataa ttcgaaactg ccgttaaata
      781 ccatattttg cgcccggact gccaaaatat gcataaagca aaagatcgcg cagcaaacta
      841 ccctgacagg aagtgtaaat aatgttttca tacgggagaa ttaatgaata cggcataaaa
      901 gtagagatgc ccttatcgca ggtcaagtca cattgagcga catgccatat atttcctgtg
      961 aagtgccctg attaattagg gagtgagttc tgcaaacata tttttcgttt tatccacatt
     1021 tcatgcagcc tgccatctga tttgactttc aacgagtaac tttgaccttc aattcaccat
     1081 catggccgaa gcattagcaa ccgttgaaca ggctaaacaa ctgggtttat tacccgagga
     1141 gtttgcccat atcacctcca ttctgggcag aacacccaat ttcactgaac ttagcattta
     1201 cagtgtcatg tggagcgagc attgttcata taaaaacagt atccgattgc tgaaaacact
     1261 gcccagaagc ggtaaaatga tgttggtgga agccggtgaa gagaatgccg gactcataga
     1321 cattggcgac ggtctggcct gcgcatttaa gattgagagt cataatcacc catcagcaat
     1381 cgaaccttat cagggggctg caacgggtgt tggtggtatt cacagagaca tttttaccat
     1441 gggagcaaga ccgatagcat cgctgaactc gctgcgtttc ggaaatattg ccaatacgcg
     1501 cacccaacat ttgatgaagg gcgttgttaa aggtattggt gattacggca actgttttgg
     1561 ggtgcctacg gttggaggtg aggtgtattt tgaggactgt tacaatacga atatcctggt
     1621 aaatgccatg agtgtgggca ttgtgaaagt aggagaaaca gtatctgcca ttgccgaagg
     1681 tgcaggtaat ccggtttaca ttgttggttc agctacagga aaagacggta tccatggcgc
     1741 aagttttgcc agtggcgatt tacatgagga gagtcatgaa gatttgcctt ctgtgcaggt
     1801 tggcgatcct ttccaggaga aattgctgtt ggaagcaacc ttggaagcga taaaaaccgg
     1861 tgctatcgtt ggcatgcagg atatgggtgc tgcaggcatc acctgttcaa catccgaaat
     1921 gagtgcgaaa ggcaaacatg gcatgaaaat caacctcgac aaagtgccta cacgccagca
     1981 aaatatgaag ggttgggaga tattactttc cgaaagtcag gaacgcatgc tcgttattgt
     2041 gcacaaggga cgtgagaaag aaattgaagc ggtattcgat aagtgggatt tacactgcga
     2101 gattattggt gaagtaatag aaggtaatac attgtattac tacatgcatg gcgaattggt
     2161 tgcggaagtt cctgcagaca gtttggtgtt gggaggcggt gcaccaattt atacgcgcga
     2221 aacaaaagaa ccggaatatt taaaagatat tgcgaaata
//
LOCUS       DAOQHY010000313         2228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_36505_length_2228_cov_4.209388, whole genome shotgun sequence.
ACCESSION   DAOQHY010000313 DAOQHY010000000
VERSION     DAOQHY010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36505_length_2228_cov_4.209388"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="PLL28_14980"
     CDS             complement(<1..1173)
                     /locus_tag="PLL28_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70684.1"
                     /translation="MKKALSIIGIILIASGKLFSQGYYNHTWLLGNFFFLQDPKGRMV
                     IDSAGYTHSVEYRKMPFLGTEATICDAQGNFLMSTNGIWIANANNDTMMNGAGLNPGG
                     VTGSWPYGLPMSSNAVFLPYPGDSTKYVLFHHTAVSNGSYYPAYDLLYTVIDITGDSG
                     LGEVITKNQVVFSDTLGWGIASCKHANGRDWWIAVQKDNSDVVFSLLFTPDGIDTVLT
                     QQLNVTPSLGSTRQISFSPDGNKFVTNKTDGGTIIDNFVQILDFDRCTGVFSNPHEVD
                     VSNGGIGWGLCFSPTTKFVYTCSSSKVYQIDLSTYVADTIATYDGFISPPNLTCCATS
                     FWNMYLAANGKIYITSGSGVQHLHEMNYPDSAGLACDVQQHSINLGYAQLRAVPNHP"
     gene            complement(1170..>2228)
                     /locus_tag="PLL28_14985"
     CDS             complement(1170..>2228)
                     /locus_tag="PLL28_14985"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70685.1"
                     /translation="ELFLSNINLQNDSGLGEVINKNQLILSDSIAWGIGCVKHANGRD
                     WWILMTKHNTSSVVKILLTPNSSSYSIQSLPNAHYTWFSASQIPFNSNGDRFAYTSYD
                     SISHDSYVVTANFDRCTGNIYNDTTIQLTYGSYLWGTAFSASGKYLYACSSSKVFQID
                     TDSLTVDTVAYYDGFISPPNLTCCATSFWNMYLAANGKIYITSGSSVQHIHEMNYPDS
                     AGVACDLQQHAIDLGSYLHLRAVPNHPNYNLGPVVGSVCDTLGVGLPEMQHDFHFGIS
                     PNPVAEGPVKIIYLLPENKSGIFEVYNMTGQLVYKMNLPPWSTLQYVDLPELSEGVYT
                     AVVRSGYERSAKKLAIIR"
BASE COUNT          650 a          493 c          439 g          646 t
ORIGIN      
        1 agggtgattg gggacagctc gtaattgagc atagcccaga ttaattgaat gttgttgaac
       61 atcgcatgcc agtcctgcac tatcagggta attcatctcg tgtaaatgct ggactccgct
      121 tccgctcgtt atatatattt taccatttgc ggcgaggtac atgttccaga acgaagtggc
      181 gcaacaggta agatttggag gagatatgaa cccatcgtaa gtagctattg tatcagcaac
      241 atatgtggac agatctattt gatacacctt gctgctgcta caggtgtaaa caaatttagt
      301 tgtaggacta aaacataatc cccatccaat ccctccattt gaaacgtcta cttcatgagg
      361 gtttgaaaaa actcctgtgc atcggtcgaa atcaagtatt tgaacaaaat tatcaattat
      421 tgtcccacca tctgttttat ttgttacaaa cttatttccg tcaggtgaaa aacttatttg
      481 acgtgtactt ccaagtgaag gagtgacatt cagctgttgt gttagcactg tatcgatacc
      541 atctggtgta aataataatg aaaagacgac atcactattg tctttctgga cagctatcca
      601 ccaatctctg ccattcgcat gcttacatga tgcaattccc caacctaaag tatcactaaa
      661 cacgacttgg ttttttgtaa ttacttctcc aagtccactg tcacctgtta tatcaattac
      721 agtatatagt aaatcatatg ccgggtaata ggaaccattt gatacagcgg tatgatgaaa
      781 caacacatac tttgttgagt cgcccgggta cggtaaaaaa acagcatttg aactcatcgg
      841 caatccatac ggccaactac cggtaactcc gcctggattc aaacccgcgc cgttcatcat
      901 cgtatcgtta ttcgcattgg cgatccagat accgttggtg ctcatcagga aattcccctg
      961 agcatcgcag atggtggctt cggtgccaag gaatggcatt ttcctgtatt caacagaatg
     1021 ggtgtatccg gcagaatcaa ttaccatccg gcctttggga tcttgtaaga aaaagaaatt
     1081 cccaagaagc caggtatgat tataataccc ctgagagaac aacttgccgg aggcgatcag
     1141 tattattccg atgatgctaa gtgccttttt catcgaatga ttgccagttt cttcgcactc
     1201 ctttcatacc cactccttac cactgccgta taaacccctt cgctcaactc tggtaaatca
     1261 acatactgca acgtgctcca gggtggtaaa ttcattttat atacgagctg acctgtcatg
     1321 ttatacactt caaagatacc tgatttgttt tccgggagca ggtaaataat ttttaccgga
     1381 ccttctgcaa caggattggg tgaaatcccg aaatgaaaat catgctgcat ctccggcaat
     1441 ccaactccca gcgtatcaca tacactaccc accacaggac ctaagttgta gttagggtga
     1501 ttggggactg ctcgtaaatg aaggtaactt cccaaatcaa tcgcgtgttg ttgcagatca
     1561 caagctacac cggcactatc cgggtagttc atttcatgaa tgtgttggac ggaacttccg
     1621 ctggtgatgt atattttacc atttgccgcc aggtacatgt tccaaaacga agtggcgcaa
     1681 caggtaagat tgggaggtga aatgaatcca tcgtaatatg cgacggtatc taccgttaaa
     1741 ctatccgtat caatctgaaa caccttggag ctggaacagg catacaagta ttttccgctt
     1801 gcactaaagg ccgttcccca caaatagctt ccataagtca actgaatggt agtgtcgttg
     1861 taaatatttc ctgtgcagcg gtcgaaatta gctgtaacaa cataactatc atgacttatt
     1921 gaatcatatg aagtataagc gaaacgatca ccgttactat taaacggaat ttgagatgca
     1981 ctaaaccatg tatagtgtgc attaggtaac gattgaatgc tataactaga actattcgga
     2041 gtaagtaaaa ttttaactac actacttgta ttatgctttg tcattaatat ccaccagtct
     2101 ctcccattgg catgcttcac acagccaatt ccccaagcta tggaatcaga tagaatcaac
     2161 tggtttttat ttatcacttc tccgaggccg ctatcatttt gaagattgat atttgataga
     2221 aaaagttc
//
LOCUS       DAOQHY010000314         2226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_36565_length_2226_cov_6.770613, whole genome shotgun sequence.
ACCESSION   DAOQHY010000314 DAOQHY010000000
VERSION     DAOQHY010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36565_length_2226_cov_6.770613"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1541)
                     /gene="mnmE"
                     /locus_tag="PLL28_14990"
     CDS             complement(132..1541)
                     /gene="mnmE"
                     /locus_tag="PLL28_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNF70686.1"
                     /translation="MMIPYLTLDDTITAISTAPGVGAIGVVRLSGRDAVSVASRIFKG
                     KNLSRQKSHTAHFGKIIDLEGREVDEVVTTIFLHPNSYTGEDVIEISAHGSPYILSRI
                     LELCIEAGARAALPGEFTQRAFLNGKMDLAQAEAVADLIAAESAAAHHTALNQLRGGF
                     SRDLDDLRTKLVEFASLIELELDFSEEDVEFADRKQFLVLIDTLKEKTSSLLESFRLG
                     NVIKKGISVAIIGKPNAGKSTLLNALLNEERAIVSDIAGTTRDTIEETLNINGILFRL
                     IDTAGIREHTTDIIESIGMERSRNNATQSDIILYLMDMNSLVSFDDRQNIYDEFQWLK
                     EFSSKTIPVYTKYLLWKENISTDSSIESADSPYILTDAKSGLGIESLKQVIFEKAVGG
                     KINTDQTIITNVRHRDILQKISVSLDKIKTGLNESLSGDLLAIEIHECLRYIGELTGR
                     VDIDRDILGTIFSKFCIGK"
BASE COUNT          621 a          437 c          475 g          693 t
ORIGIN      
        1 taaagctaat aacaacataa aacaacaaat aaatgaaata ttggaaagta aagttaaaaa
       61 ttaaaaaacc tttgtttata tgacttttat tcacttttat ttgttcttgt tttgtgttgt
      121 taaatattgc tttactttcc aatgcaaaac ttgctgaata tcgtacccaa aatatcccgg
      181 tcaatgtcca cgcgtccggt caattctcca atgtatcgca ggcattcgtg aatttctatg
      241 gcaagcagat caccactaag actttcgttg aggccggttt ttattttgtc gaggctaacc
      301 gaaatttttt ggagaatgtc gcgatgccgc acatttgtga tgatggtttg gtcagtattg
      361 attttgcctc ctactgcctt ttcgaagatg acttgcttga gggattcgat ccccaagccg
      421 gacttggcat cagtaaggat atagggagaa tcagcacttt cgatagatga gtcggttgaa
      481 atgttttcct tccataataa gtatttcgta tagaccggga tggtcttgga cgaaaattcc
      541 tttagccatt gaaattcatc gtagatgttc tgtctgtcat caaagcttac aaggctattc
      601 atgtccatga ggtagaggat gatatccgat tgggtggcat tgttgcggga acgctccata
      661 ccgatacttt cgatgatatc ggttgtatgt tcacggatac cggcagtgtc gatgagtcgg
      721 aacaggatac cgttgatgtt gagggtttct tcgatggtgt cgcgcgttgt accggctatg
      781 tcgctgacaa tggcacgctc ttcattgagc agggcattga gtagggttga ttttccggca
      841 ttgggcttgc cgatgatggc tacgctaatg cctttcttga tgacattgcc cagtcggaac
      901 gattccagca gggagctggt tttttccttg agggtatcga tcagcacaag aaactgtttc
      961 cggtcagcga attccacatc ttcctcacta aagtcaagtt ccaattcaat taatgaggca
     1021 aattctacga gctttgttcg caggtcatca agatctcttg agaatcctcc gcgcagttga
     1081 tttagtgctg tatgatgagc agcagcactt tctgccgcta tcaagtcggc tacagcctct
     1141 gcttgtgcta ggtccatctt gccattgaga aaggcacgct gagtaaactc accgggtagc
     1201 gcagctctcg cacctgcctc gatacaaagt tccagaatgc gcgaaaggat ataaggtgaa
     1261 ccatgagcgc tgatttctat gacatcctca ccggtatagc tgttgggatg gagaaaaatg
     1321 gtagtcacca cctcatcaac ttccctacct tccaaatcga tgatcttccc aaagtgtgcc
     1381 gtgtgacttt tttggcggga caaattcttt cccttgaaga tcctgcttgc aacgcttacc
     1441 gcatccctgc cactaagccg tacaacaccg atagcgccca ctccgggagc tgtggaaatg
     1501 gcagtaatgg tatcatccag agtcaagtat ggtatcatca taataaaggg tgaaattagt
     1561 aaaaaagggg aattcattgt gaaaggaaag aaattggtgt ggatcacgta tgccctttac
     1621 tctttccgga ctaaaagagg agaagttgtc aatgaggcat gtaactcgga tcagctatct
     1681 tccaataatt atttaagtct aatgtttcga atgtccttaa ttcttgataa atacttatca
     1741 aatgtcaaaa ctgtggaatg attttgaact attaatgatt gataagaata ggtatataaa
     1801 aaacccgatg cttgttaaat actgcttaat ggtatacttc agcataatcc ttttactttt
     1861 ttactgttca tcgtcatata tgccaccgaa gtaatcaaat attatggcca ttggctcatc
     1921 atcctattaa tttgtagata aagcacaaag cccgggtgac ggtttggctc ttaagacata
     1981 aatgtggttg agcctgcttt ttctaattat tcaatgccat tagcgttatg tcatgctcca
     2041 atttaaattg ttgtaatccc gcgatccgaa aaggttgtaa ttcaggcaaa ggcaaattgt
     2101 aaattcaatc tgagggttca accattgcaa ttaataggta ttaatttttc gcttcatttt
     2161 cagatttgat aaacaggaat aaaatattta aacaaatgat taaaatattt ggttaatttg
     2221 gttaat
//
LOCUS       DAOQHY010000315         2214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_36910_length_2214_cov_3.671144, whole genome shotgun sequence.
ACCESSION   DAOQHY010000315 DAOQHY010000000
VERSION     DAOQHY010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36910_length_2214_cov_3.671144"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..679)
                     /locus_tag="PLL28_14995"
     CDS             complement(125..679)
                     /locus_tag="PLL28_14995"
                     /inference="COORDINATES: protein motif:HMM:NF015810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HNF70687.1"
                     /translation="MKSKVSALMDGELDQQDVLNIIEAIKKDNNLRDEWETYHLIGDA
                     LRQSSRLSMNISSSVSHKLKAEPTVLSPNTSRITEKKQTHKVLAFSMAASIIAMISGW
                     VIMHNLYKPQQIIVAEQSKRENTLTASPVVVSSPPLIHNYSHPPVEMNDYLFVHREFS
                     PGVTMNGQVTNVNNVTEYHERYGR"
     gene            complement(681..1280)
                     /gene="rpoE"
                     /locus_tag="PLL28_15000"
     CDS             complement(681..1280)
                     /gene="rpoE"
                     /locus_tag="PLL28_15000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HNF70688.1"
                     /translation="MGDREIDQQLVERVQGGDKHAFDLLVIKYQRKLARLLSQFIRDA
                     AEVEDVTQEAFIKAYRALPSFRGDSAFYTWLYRIGINTAKNFLVSQGRKLPTLQGFDN
                     EDAEDFEDSGLLKELNTPESELMSQEIAQTVNQTLDSLPEELRTAIVLREIDGLSYEE
                     IANIMSCPIGTVRSRIFRAREAISEQLRPLLGTSKDKRW"
     gene            1542..>2214
                     /locus_tag="PLL28_15005"
     CDS             1542..>2214
                     /locus_tag="PLL28_15005"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNF70689.1"
                     /translation="MSNNDFDTLIIGSGLAGFTLALHLAPYKKVCIVTKQTADSGASS
                     WAQGGIAAALSTNDSPAKHVHDTLVAGAGLCDEEVTRYVAENAANAIYWLINQGVIFT
                     SDRTSETGYHLTKEGGHSMRRIIHSGDATGKAVQQALIQKIRNHPNIAVLEHHIAIDL
                     ITSDKLSGYTDLKQKRCFGAYILDKKKDKVLTFTAQNTVLATGGASKVYLYTTNPDTA
                     TGDGIA"
BASE COUNT          662 a          500 c          423 g          629 t
ORIGIN      
        1 gccatttttt ttaaccagtc aagcgcactt tccgttgagc gagataattc ttgtgcgcat
       61 gctgtttgaa aactgattgc cgctaataaa aacagcgaaa acagcgcgaa tattctgcgg
      121 ctcatcatct cccatatctt tcgtggtatt ccgtcacgtt attgacgttg gtaacttgac
      181 cattcattgt gactcctgga gaaaactcac ggtgcacaaa caggtagtca ttcatttcaa
      241 cgggaggatg agaataattg tgaatcaagg gaggggaaga aactacaacg ggggaagcgg
      301 ttagcgtatt ttcacgtttg gattgctccg ccacaattat ctgttgcggt ttatataaat
      361 tatgcataat tacccatcct gagatcatcg cgatgatcga agccgccatc gaaaacgcca
      421 ggactttatg cgtttgtttt ttttctgtaa tgcgagaggt attgggagag aggacagtcg
      481 gctcagcttt taacttgtga ctaacactgg aagaaatatt cattgataat cgtgaagatt
      541 gccgtaacgc atcaccgatc aaatgatacg tttcccactc atcgcgcaaa ttattatcct
      601 tttttatcgc ttcgataata tttaaaacat cttgttgatc aagttcacca tccattaatg
      661 cagatacttt acttttcaca ttaccacctc ttgtctttac ttgtccctag caaaggacgc
      721 aactgttcgg atatcgcttc acgtgcacga aatatacgtg aacgtacggt ccctatggga
      781 caactcataa tattggcaat ttcttcataa cttaacccat caatttctct taaaacaatt
      841 gcagttcgca attcttcagg caatgaatcc aatgtctgat taaccgtttg agcaatttct
      901 tgactcatca gttcactttc aggtgtattc aattccttca gcaaaccgct atcttcaaaa
      961 tcttcagcat cctcattgtc aaatccctgc aatgtcggaa gcttgcgtcc ttgggacact
     1021 agaaaattct tcgctgtatt aataccaatg cgatacagcc atgtataaaa agcactatct
     1081 ccacgaaatg aaggcaaagc tctatatgcc ttaataaagg cttcttgtgt tacatcctca
     1141 acttcggccg cgtcgcgtat aaattgcgat agtaagcgcg ccagcttccg ttgatactta
     1201 atgactagta gatcaaatgc atgcttatct ccgccttgaa ctcgttctac taactgttga
     1261 tcgatttccc gatcccccat acaacttggc ccctgagcat tttgaataaa tttaaattta
     1321 ctcttgttct gttattaaat gttgcataat ggagcaacag aataagtata catatccaaa
     1381 tgaatctcgg aaaccagtaa tggcatcgca atcgttccaa agacaattaa tcttatcaat
     1441 tagttcacaa tccagttttt gtcttcaaaa aataaaacaa tacgtaattt cacactcgct
     1501 gttttttaac ccattaatta caagaacata aaccctactt aatgtctaat aacgatttcg
     1561 ataccttaat tattggcagc ggactggctg gatttacact cgcactgcat ttggcgccct
     1621 ataaaaaggt ttgcatagtc accaaacaaa ccgctgattc aggcgccagc tcttgggcgc
     1681 aaggcggaat tgcagcggca ttatcaacca atgattctcc agcaaaacat gtgcacgata
     1741 cgctagtcgc cggggccggt ttatgcgatg aggaagtcac tcgatatgta gccgagaatg
     1801 cggcaaatgc aatctattgg ttgataaacc aaggtgttat tttcaccagc gaccgaacca
     1861 gtgaaacggg atatcaccta acaaaagaag gcggtcacag catgaggcgc atcattcata
     1921 gcggtgatgc cacaggaaaa gctgttcagc aagctctcat ccagaaaata cggaatcatc
     1981 cgaacatagc ggtattggaa catcatatcg ccattgattt gattaccagc gacaaattat
     2041 cgggctacac agatctaaag caaaaacgat gttttggcgc ttatatcttg gataagaaaa
     2101 aagataaagt cctgacattc accgcgcaaa ataccgtgtt agcaactggg ggagccagca
     2161 aagtttatct atataccacc aatccggata cagccacagg tgacggtatt gcca
//
LOCUS       DAOQHY010000316         2172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_38158_length_2172_cov_4.668399, whole genome shotgun sequence.
ACCESSION   DAOQHY010000316 DAOQHY010000000
VERSION     DAOQHY010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38158_length_2172_cov_4.668399"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PLL28_15010"
     CDS             <1..494
                     /locus_tag="PLL28_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70690.1"
                     /translation="LIGGDELPFPGLTLRAGITDKLDAGICWSKSPGANYSIAGAQVQ
                     YQVLGDTVKHWNIATRLGFTSLYGPEDLGYVVFGLDMLASKEFPFMQDHLALTPYALV
                     STDFVHAREKSEIVTLDDENVMGAQAALGLEAKIYVVRLGVEYNLSAVNTLSYKLGVR
                     VAF"
     gene            complement(550..1062)
                     /locus_tag="PLL28_15015"
     CDS             complement(550..1062)
                     /locus_tag="PLL28_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70691.1"
                     /translation="MQLNIPGFIFMALLVFLVKKTSTDIPTVTDESQVLYRPSRSFGV
                     LGMLLFFLGIVLFTLILLGELPSNGYTGAFFLTFFCISGLLLTYFRMAVYLKTTGEAL
                     IKRNIFKKEIIIPYKEIVSLSYNKNISALIVKTEVEYLFCFNNLIGFNFFIQTLEEAT
                     GLEAKGWMTR"
     gene            complement(1191..1700)
                     /locus_tag="PLL28_15020"
     CDS             complement(1191..1700)
                     /locus_tag="PLL28_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70692.1"
                     /translation="MQNWIQTTILVIVILAISVALRKSAHAEAGKKDGMSLYKAPVIF
                     KYIGFGLLAFGVYVLGDTIFGNSINQHSMNYLLAGIFVATGGLLIYFQRTFYITIDAH
                     QVVKHKMFHKEITIQLNEIQYIRYRKLMKDMIIISANGKMIIDESVTGFMSLVNELKE
                     KTGVDIDNL"
BASE COUNT          690 a          420 c          384 g          678 t
ORIGIN      
        1 acctgatcgg tggtgatgaa ttgccatttc ccggacttac ccttcgcgca ggcataacag
       61 ataaacttga tgccggaatt tgctggtcga aaagtccggg tgctaattac agcattgccg
      121 gagcccaagt gcaatatcag gttttaggcg atactgttaa gcattggaat attgcaacgc
      181 gattgggatt tacttcgcta tatggccctg aagatcttgg atatgtagtg tttggattag
      241 acatgctggc gagtaaagaa tttcctttta tgcaagatca tctcgcgcta acaccttatg
      301 cgcttgtcag taccgatttt gttcacgctc gtgaaaaatc agaaatagta acgcttgacg
      361 atgaaaatgt aatgggcgct caagcagcgc tgggactgga agcaaaaata tatgtggtgc
      421 gtttgggggt ggaatataat ttgtcggcgg ttaatacatt gtcgtacaaa ttaggtgtgc
      481 gtgttgcatt ctaatttatc taagttgcaa ggatagggtt tacggtgtta tcctcgcagc
      541 ttttttatgc tatctagtca tccaaccttt tgcctctaat ccggttgcct cctccaatgt
      601 ctgaatgaaa aaattaaatc caatcaaatt attgaagcaa aataaatact cgacctctgt
      661 ttttacaata agggctgaaa tatttttgtt gtaggatagt gaaacaattt ccttatatgg
      721 tatgataatc tcctttttaa agatgtttct ttttattaat gcctcaccgg ttgttttcag
      781 ataaactgcc attcggaaat aagttaataa caatccggat atgcaaaaaa aggtaagaaa
      841 aaacgctcca gtatatccat tacttggcaa ctcgccaagc aggataagcg tgaacagcac
      901 aattcccaga aagaacaaca acatacctaa cacaccaaaa gaccgagaag gtctgtaaag
      961 cacttgtgat tcatcagtaa ctgttggaat gtcggtggat gtttttttaa ccagaaacac
     1021 caacaaggcc ataaaaatga atcccgggat attaagttgc atcttgtatg tgttttagac
     1081 actagtgaaa tttactcgcc gaaaccattc accaaattat caaccgaaag ttacatcatc
     1141 cgaaacaaaa tacatcgaat tggcttcagg ttaagatact acacctgaac ctacaaattg
     1201 tcgatatcta ctccggtttt ttcttttagc tcattcacca ggctcataaa accggtaaca
     1261 ctttcatcga ttatcatttt tccgttagcg gatataatga tcatatcctt catgagtttt
     1321 cggtaacgga tatattgaat ctcgtttaat tgaatcgtaa tttctttatg gaacatttta
     1381 tgctttacaa cttgatgtgc atcaattgtg atataaaatg ttctttgaaa ataaattaat
     1441 aaacctcctg ttgctacaaa tattcctgca agcaagtaat tcatactgtg ttgatttata
     1501 gaatttccaa atatcgtatc accaagcaca tacaccccaa atgctaataa tccaaagcca
     1561 atatatttga aaatgacagg tgccttgtaa agcgacatcc cgtccttctt accggcctca
     1621 gcatgtgcag atttgcgcaa tgcgactgaa attgccaata tgactataac aaggattgtg
     1681 gtttgtatcc aattttgcat cctcttttat tatgtgcaga agcaatgttg cgtacctagc
     1741 gaatatttgt aatgatacca aaattgccac acatatcggg aatttcataa ttagcgtctc
     1801 tttaatctaa atgtattagg caagtttgtt actaaattcg attaataaat gcatgtcgca
     1861 attccggcaa ataatttcaa ataccgctca attgtcatat ccaatgatgg atttccggaa
     1921 aaccaaaccc ctatgttata aagtattgga aaccgcaaaa aatatttttt tttcaaaata
     1981 ttcatccaaa tccttaaaat ttttcaagta gtgccaagga atcatccaat tttgtaagaa
     2041 tcaattcact aacctgcaat atctatttac accaatttca taatgatctc ggtcatttta
     2101 agaggtgttg aataatagta gttttacgcc acaaccacat ctaaaatgga acaacataaa
     2161 tttcgccccg ag
//
LOCUS       DAOQHY010000317         2167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_38315_length_2167_cov_4.016572, whole genome shotgun sequence.
ACCESSION   DAOQHY010000317 DAOQHY010000000
VERSION     DAOQHY010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38315_length_2167_cov_4.016572"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PLL28_15025"
     CDS             complement(<1..262)
                     /locus_tag="PLL28_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HNF70693.1"
                     /translation="MAISEADILKALSNVDDPDLHKDIVSLGMVKDITIDGTHIRFTV
                     ELTTPACPMKEEIRRACVNAVKLLVSKEAEVDVNMSARVSSIR"
     gene            404..>2167
                     /gene="topA"
                     /locus_tag="PLL28_15030"
     CDS             404..>2167
                     /gene="topA"
                     /locus_tag="PLL28_15030"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNF70694.1"
                     /translation="MGKNLMIVESPAKAKTIEKFLGKDFVVKSCYGHIRDLEKADFGI
                     DFKNNYEPKYIISADKEAVVKELKKLAKSAEEIWLATDEDREGEAISWHLCEVLGLNA
                     KEAKRIVFHEITKKAIQNAVANPRNINIDLVDAQQARRILDRIVGFELSPLLWRKINM
                     KTSLSAGRVQSVAVRLIVEREQEIGKFNAMASFKVTALFHVKDDSGKTYQLKAELPAK
                     LDEEAQVKSFLQDCIGAEFQVGNITVKPAKRSASAPFTTSTLQQEASNKLGFSVSRTM
                     IIAQKLYEEGHITYMRTDSVNLSQQAIDAAQSEIIKNYGERYSNPRQYKTKSSGAQEA
                     HEAIRPTYMENQDVPGDFDEKRLYKLIWKRTMASQMADAELERTIVDIDISTRKEKLA
                     AKGEVIKFDGFLKVYTEALEDERDEDATILPPLKVGAPLTMIEMEGAQKFSKPPARYT
                     EASLVKKLEELGIGRPSTYASTISTVQKREYVVKKDKEGIAREAVQFKISEKQVVRSV
                     VTEMYGAERMKLFPTDLGILVTEFLIENFPQILDYSFTADVEAQFDDIAIGKREWHKM
                     IDAFYKPFHEHLELTTKTAQRV"
BASE COUNT          680 a          411 c          505 g          571 t
ORIGIN      
        1 cacggataga cgatactctt gcactcatgt taacgtcaac ctctgcctcc ttgctaacca
       61 gcagcttaac agcattgaca catgccctgc gaatctcctc cttcatgggg caggcaggcg
      121 ttgtaagttc aacggtgaag cggatatgcg tgccgtcgat ggttatgtct ttcaccattc
      181 ccaggctcac aatatccttg tgaagatcgg ggtcgtcgac gttagaaagg gcctttaaaa
      241 tatctgcttc tgaaatcgcc atgccttaaa aatttgggat gtaaagttac gatgcagaca
      301 acaaaaagcc tcctttatta gtttgatatg cgagttgtta aagtgcataa aaagttgttt
      361 tgttgttcag aaaactatat ttgcacccta gacaagtgct gacatgggga aaaatttgat
      421 gatcgttgag tcgccggcaa aggcgaaaac cattgagaaa ttcttaggaa aagatttcgt
      481 ggtaaagtct tgttatggcc atatccgcga cctggagaaa gccgattttg gtatagactt
      541 taagaataat tacgaaccga aatacattat ttctgccgat aaggaagctg ttgttaaaga
      601 gctgaagaaa ttggcaaaaa gcgcagaaga aatatggtta gcaacggatg aagaccgcga
      661 aggagaagct atatcgtggc atttgtgcga agttttgggc ttaaatgcca aggaagcaaa
      721 acgcatcgtt tttcacgaga ttaccaaaaa agctattcaa aacgctgttg ccaatccacg
      781 taatattaat atagaccttg tcgacgctca acaggcacgc cgtattctgg accgtattgt
      841 gggttttgaa ttgtcgccat tattgtggcg taagataaac atgaagactt cgctttctgc
      901 cggtcgcgtg caatcggtag ctgttcgttt gatcgttgag cgtgaacagg aaatcgggaa
      961 gttcaatgca atggcatcat ttaaggtgac tgcattgttt catgtgaagg atgatagcgg
     1021 gaagacttat caattaaagg ctgaattacc tgccaagctt gatgaagaag cgcaggtgaa
     1081 atcattttta caagactgca taggtgctga atttcaagtg gggaatatta cagtgaaacc
     1141 cgcaaaacgc tcggcatcag cgcctttcac aacatctacc ctgcaacagg aagcgagtaa
     1201 taaattagga ttttcagtat ctcgcaccat gattattgcc caaaaattgt atgaggaagg
     1261 gcatattact tatatgcgta ccgactcagt caacctgagt caacaggcca ttgatgcggc
     1321 gcaatctgag atcattaaaa attatggcga acgatattca aatcctcgtc aatacaaaac
     1381 aaaatcaagt ggtgctcagg aagcgcacga ggcaattcgt ccaacgtaca tggaaaatca
     1441 agatgtgcca ggcgattttg acgaaaagcg attatataaa ttaatttgga agcgcacaat
     1501 ggcatcgcaa atggctgatg ctgaattgga gcgcacgata gttgatattg atatttcaac
     1561 gcgcaaggaa aaacttgcag caaaaggtga ggtgataaag tttgatggtt tcctcaaagt
     1621 ttacactgaa gctttggaag atgagcgcga tgaagatgct actatacttc ctccattaaa
     1681 agtaggagca cctctaacca tgattgaaat ggaaggtgcg caaaaattct ctaagccacc
     1741 tgcgcgatat actgaagcta gcctggttaa aaagcttgaa gaattaggta taggtcgacc
     1801 atcgacatat gcatcgacaa tttctactgt tcaaaagcgc gagtatgttg taaaaaaaga
     1861 taaggaaggt attgcccgcg aagctgttca gtttaaaata agtgaaaagc aggttgtgcg
     1921 cagtgtcgta acagaaatgt atggcgctga acgcatgaaa ttatttccta cggatctggg
     1981 aattttagtg actgaatttt tgatagagaa ttttccgcag atattagatt atagttttac
     2041 tgccgacgta gaagcgcagt tcgatgatat tgcaataggc aagcgcgagt ggcataaaat
     2101 gattgatgcc ttttacaaac catttcatga acatctcgaa ctcacaacca aaacggcgca
     2161 acgcgtt
//
LOCUS       DAOQHY010000318         2131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_39494_length_2131_cov_4.561175, whole genome shotgun sequence.
ACCESSION   DAOQHY010000318 DAOQHY010000000
VERSION     DAOQHY010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39494_length_2131_cov_4.561175"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            326..>2131
                     /locus_tag="PLL28_15035"
     CDS             326..>2131
                     /locus_tag="PLL28_15035"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNF70695.1"
                     /translation="MRKLILALFLIPTCVFAQQNETVSASIDRTILDSLHMVLRAEIL
                     NLEAAKQKALAMGLPVSGSGEDGAFMSIRSLRNGQIMYYNTTNLNSAKTDGSNKLWSG
                     GGLGLSLNGSGIVARVWDAGAIKTTHQELNGRASMGVSQTATLSDHATHVSGTIAAFG
                     VDASAKGHANQAEIKGFDWDFDTDEMTTEAGNGMLVSNHSYSYIAGWYFNGGTGQWEW
                     YGDGTVSTVEDYIFGNYLDESRNVDLIVNASPYYLPVIAAANDRDDNVPTGATYKMIW
                     DGTIHTADGTQPNPDGTYDCIPGGLQTAKNTLTVGAVNDIVNGYLAPGDVVLTGFTSY
                     GPCDDGRIKPDVVANGTSLHSCVSTSTTSYSDFSGTSMAAPSVTGSVVLLQQHHHNIF
                     GNYMLNATAKAVIIHTVDKASATFGPTYSYGWGLMNTDGAALLISDALIHPIRIQERN
                     LPNGGTYNFTGNSNGSEPLKITIAWNDPAASVPTPALDPVTKMLVNDLDLRVTRLSDN
                     TVFYPYKLNPASPGTAAITGDNNTDNVEYVYLATPSAGDYKITINHKGTLSGGDQDYT
                     IVVSGLSCIPYSLTENVTITAPATYTLPNGSIVSTS"
BASE COUNT          637 a          421 c          505 g          568 t
ORIGIN      
        1 cgaaggcgat gtttgaggtg ggagggcagt atcgacggaa tatgtgagaa aaggaaaaag
       61 gagagaggag agaggagaga ggagagagtt tgtttcacgc agagacgcag aggcgcagag
      121 aggtgataca tcaatgtaac gaagttacat tgagtttggg ccgtctcact acccactacc
      181 tgcctgacgg caaggcaggc tcactactca ctaccagttc cgagacacca aaccttgtta
      241 ttgtgacatt cgattacagc ttactttaga atggtatcgc tttcctaagg aatgattttc
      301 taatttagag ttcaaataat gtatcatgcg caagcttatc ctcgctttat ttttgattcc
      361 tacatgtgtt tttgctcaac agaatgaaac agtatcagca tcgatagatc gtacaatact
      421 tgattcattg catatggtgt tgcgcgcaga aatattaaat cttgaggctg ccaagcaaaa
      481 agcacttgca atgggtttgc ccgtttccgg ttcaggtgag gatggtgctt tcatgagtat
      541 tcgtagtttg cgcaatgggc agatcatgta ttataatact accaatctga attccgcaaa
      601 gaccgatggc agcaataaat tgtggtcggg tggtggactt ggattgagtt tgaatggttc
      661 gggaattgtg gcgcgcgttt gggatgcagg tgcaataaaa actacgcacc aggaactcaa
      721 tggaagagca tcaatgggtg tttcgcaaac ggcaacactc agcgatcatg ctacccatgt
      781 ttccggaact attgccgcat tcggtgttga tgcttcggca aaaggacatg ccaatcaggc
      841 tgaaataaaa ggtttcgact gggatttcga taccgacgaa atgaccactg aagcaggaaa
      901 tggaatgctg gtgagcaatc atagttacag ctatatagca ggatggtact tcaatggtgg
      961 tacaggacaa tgggaatggt atggcgatgg aactgtgagc accgttgaag attatatttt
     1021 cggaaattat ctggatgaaa gtagaaatgt agatttaatc gtaaatgcaa gtccgtatta
     1081 tttacctgtt attgcagccg caaatgatcg cgatgataat gtgccgacag gagcaactta
     1141 taaaatgata tgggatggca ccatacatac tgctgatggc acacagccaa atcctgatgg
     1201 cacctatgat tgtattccag gaggattgca aacagcaaaa aatacactaa ctgttggcgc
     1261 agtgaatgat attgtaaatg gttatctcgc acccggcgat gttgtattaa ccggattcac
     1321 ttcttatggt ccttgcgatg atggaaggat aaaacctgat gttgttgcca acggcacaag
     1381 tttacattcc tgtgtatcta caagcaccac ttcgtactcc gatttcagcg gcaccagtat
     1441 ggccgcacct tctgtgaccg gaagtgttgt attattacaa caacatcacc ataatatatt
     1501 tggcaactac atgctaaatg ccaccgcaaa agctgtgatc atccataccg tagataaagc
     1561 atctgccact tttggtccaa cttatagtta tggctgggga ctgatgaata ccgatggcgc
     1621 tgcattatta attagtgacg cgctaataca tcctattcgt atacaagagc ggaatttgcc
     1681 aaatggaggg acatataatt ttaccggtaa cagcaacggc agtgaaccat tgaaaattac
     1741 cattgcttgg aacgatcccg cagcatcagt tccgacacct gcccttgatc cggtaactaa
     1801 aatgctggta aacgaccttg atctgcgtgt aacgcgtttg agtgataata ctgtttttta
     1861 tccttataaa ttaaatccgg catctcccgg cactgcagct ataacaggtg ataataatac
     1921 cgacaatgtt gaatatgtat acctggcgac accaagtgca ggagattata aaatcacgat
     1981 taatcacaag ggaacattat caggaggaga tcaggattac actatagttg tgagcggact
     2041 gagttgcatt ccttacagtt taacagaaaa tgtgactatc actgcacctg caacatacac
     2101 tcttccgaat ggaagcatag tgagcacaag t
//
LOCUS       DAOQHY010000319         2123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_39770_length_2123_cov_4.808027, whole genome shotgun sequence.
ACCESSION   DAOQHY010000319 DAOQHY010000000
VERSION     DAOQHY010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39770_length_2123_cov_4.808027"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="PLL28_15040"
     CDS             complement(<1..490)
                     /locus_tag="PLL28_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNF70696.1"
                     /translation="MHPNEFRQYAIKHLGMSSNALDSYMNVSSMHVTNLTPYIIEERQ
                     LNAQALDVFSRLMMDRIIFLGTGIDDYVANVVQAQLLFLSSVDAKRDIQIYINSPGGV
                     VYSGLAIYDTMHYVTPDVATICTGIAASMAAVLLCAGTKGKRSALGHSRVMIHQPMGG
                     AQG"
     gene            complement(480..1859)
                     /gene="tig"
                     /locus_tag="PLL28_15045"
     CDS             complement(480..1859)
                     /gene="tig"
                     /locus_tag="PLL28_15045"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNF70697.1"
                     /translation="MNVNVENLGSQHHIVHITLDHADYKEPFEKALKEIGKKAQVPGF
                     RPGHVPAGMIRKMYGESVILDELYKLVNEQMNNWLKTNNHELLGDALPVNKDLGVDYN
                     SNKSYEFSYEVGVQPEVEVESVLRNAAAFTRYRIPATEAEIDQEVERMQRKYGERKDV
                     ESIEENDVVYAHAHQLNDDGSEQEGGMHVDTYFNLQMLNDDQKDVFLGAKSGETKNIM
                     DIFSVFKGDKVRVAKNVLQMGEVTEAQLEDFNPAFSFRIDRIARLFDAEINEAFFTEV
                     SKEVGPVTNMDELRNQIRGAIELYNDQMTDVNLENNIFTYLMDTTNVPLPDVFLRKWY
                     VQANRKSEEAEDFEKEFTEFITRLKQSLIYRKVQQTHQIEVSNEEIVQEATATVARSY
                     AQLGDDFIQYVLGSQLKDKQFVENMHDRVAQKKFFAALRGYVAVEEQSITLEAFQEMN
                     KKTEETYAS"
     gene            complement(1937..2021)
                     /locus_tag="PLL28_15050"
     tRNA            complement(1937..2021)
                     /locus_tag="PLL28_15050"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1985..1987),aa:Leu,seq:tag)
BASE COUNT          528 a          497 c          470 g          628 t
ORIGIN      
        1 gaccttgtgc accacccata ggctggtgga tcatcacgcg tgagtgaccc aaagctgagc
       61 gcttgccctt tgttccggca caaagcaata ctgcggccat agaggcggcg ataccggtac
      121 aaatagttgc tacatcgggt gtaacatagt gcatggtatc ataaattgcc aatcccgaat
      181 acacaacacc accgggactg ttgatataga tttgaatatc gcgctttgca tcaacactcg
      241 acaaaaagag gagctgtgcc tgcacaacat tggctacata atcatcgata cctgttccca
      301 ggaagatgat acggtccatc atcaaacgtg aaaacacgtc gagagcctgc gcattcagct
      361 ggcgctcttc aatgatgtaa ggagtcaaat tggtaacatg catgctgctg acattcatat
      421 agctgtcgag cgcattgctg ctcataccaa gatgtttaat cgcgtactga cggaattcgt
      481 taggatgcat atgtttcttc agtttttttg ttcatttcct ggaatgcttc cagcgttatt
      541 gattgttctt caactgctac ataaccacgc aatgctgcaa aaaatttctt ttgagccacg
      601 cggtcgtgca tattttcaac aaactgcttg tccttgagct gcgatcccaa cacatactgg
      661 atgaaatcat cgcccaactg agcatagctg cgggcaacgg tagctgtggc ttcttgcaca
      721 atctcctcat tgctcacttc gatctgatgt gtctgctgca ccttgcggta aatcagcgac
      781 tgtttcaggc gggtgatgaa ttcagtgaat tctttttcaa agtcttctgc ttcctcactt
      841 tttctgttgg cctgcacgta ccatttccta aggaagacat cgggaagtgg cacattggtt
      901 gtatccatca gataagtgaa gatattattt tcgaggttga catcagtcat ttggtcgtta
      961 tacaattcga tggctccgcg aatttgattg cgcagttcat ccatattggt aaccggacct
     1021 acctctttag atacttctgt aaagaacgct tcattgatct cagcatcgaa taaacgtgca
     1081 atgcggtcga tgcggaaact aaaggcagga ttgaaatctt caagctgcgc ctcagtcact
     1141 tcacccatct gcagcacatt tttcgctaca cgcactttgt cgcctttgaa gacggagaag
     1201 atatccataa tatttttcgt ctctccactt tttgctccca ggaagacatc tttctgatcg
     1261 tcgttgagca tttgcagatt gaaataggta tctacatgca tgccaccttc ctgttcactg
     1321 ccatcgtcgt tgagttgatg tgcatgtgca tatactacat cattttcttc aatgctttcc
     1381 acatccttgc gttcgccata tttgcgttgc atgcgttcta cctcctgatc gatctcagct
     1441 tccgtagccg gaatgcggta gcgggtaaag gctgcggcat ttcgcaatac gctttctacc
     1501 tcaacctcag gttgtacacc aacttcatag ctgaactcgt atgatttatt actgttgtag
     1561 tcgacaccca aatctttatt tacaggcaac gcatcgccaa gtaattcatg attatttgtt
     1621 ttgagccaat tgttcatttg ctcgttcact aacttgtaaa gttcatccag tattacagat
     1681 tcaccgtaca ttttacgaat cattcctgca ggcacatgtc cggggcgaaa accgggtact
     1741 tgtgcttttt taccgatttc tttcagggcc ttttcgaatg gctccttata atctgcatgg
     1801 tcgagggtga tatgcacaat gtggtgctga ctgcctaagt tttctacgtt aacgttcact
     1861 gctttggatt gaaatggtta tgataagatc ttttcggaag ctttaaccac aaaggcggat
     1921 ttccggttcc cggaggtggt gcgggtggag ggactcgaac ccccatgcct tgcggcgcca
     1981 gatcctaagt ctggtatgtc taccaatttc accacacccg ccttcgctta gccctgaaaa
     2041 aggaatgcaa aggtaggaaa aaaaagggaa tgggaatgtg aaaatgtggg aatgtgggaa
     2101 tgtgggaatg tgagaatgtg gga
//
LOCUS       DAOQHY010000320         2122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_39805_length_2122_cov_4.968070, whole genome shotgun sequence.
ACCESSION   DAOQHY010000320 DAOQHY010000000
VERSION     DAOQHY010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39805_length_2122_cov_4.968070"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..630)
                     /gene="ybeY"
                     /locus_tag="PLL28_15055"
     CDS             complement(202..630)
                     /gene="ybeY"
                     /locus_tag="PLL28_15055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNF70698.1"
                     /translation="MLGIQYTSTKTLFRLKEKERITIWLEHLMAMHHKFPGNLTFIFC
                     SDEYLLEMNKQYLNHDTYTDIITFNYNEGHYISGDIFISVDRVKENAGSFKVKFEDEL
                     HRVMAHGVLHLLGFNDKTKKDEAEMRKMEEMALGMMNVGM"
     gene            complement(665..1075)
                     /locus_tag="PLL28_15060"
     CDS             complement(665..1075)
                     /locus_tag="PLL28_15060"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF70699.1"
                     /translation="MSEQPLSKQLSLRSEPLNITLIEPFVEELRQRLHLADEVYGNML
                     VCITEAVNNAILHGNKSDATKEVKVQIRTEQNTLCCCVQDQGCGFDYNNLPDPTSPEH
                     IEEVGGRGIFLMKHLSDVVIFSNDGSSVEIQFKI"
     gene            complement(1089..>2122)
                     /locus_tag="PLL28_15065"
     CDS             complement(1089..>2122)
                     /locus_tag="PLL28_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70700.1"
                     /translation="EMAKEMQNAMQQSGMEQQQEDMNALRRLIDNLVTLSVEQEDLMK
                     EVKGMKSSNPKFVDLMAQQQKIKEDSKMVEDSLNALAKRIFQISSFITRELSAVNDGL
                     AETVEQMGDREVAKSNLYQQQVMTGYNNLALMLDEVMQQMQEQMAKSMPGSQMCQKPG
                     GSSDSQLPSMSEMQKQLNEQLNKMKGQMEQGKKPGGNQGMSQELAEMAQRQAALRQAL
                     QELAKQLGGGNTEDGKLAKQLEQIADKMDQTEEDIVNKRITQETLKRQEEILTRLLEA
                     EESDRQRKQDNERQSNTANEINRTIPPAIEEYLKKRNAELDLYKTVSPDLKPFYKNLV
                     EEYLRDINQ"
BASE COUNT          458 a          562 c          439 g          663 t
ORIGIN      
        1 attgcattcc ctattaacgc gccagcgtta aaagcacctc acacttgcta atttgctaat
       61 tctcaaattt gctaattctc aaatttgcta attaaccaca cttgctaacc tgctaaccct
      121 caaacttgct cactaagacg agcgtcttca tgcaagcaca caccccattg gctaattgtt
      181 tattcccaca ttcccacatt ctcacattcc cacattcatc atccccaacg ccatttcctc
      241 catcttcctc atctccgctt catctttttt tgttttatcg ttgaagccaa gcagatgcaa
      301 aacaccatgt gccatcacgc ggtgtaattc atcttcgaat ttaactttga aggaaccggc
      361 attttctttc acgcgatcaa cactgataaa aatatcaccg ctgatatagt gaccttcgtt
      421 gtaattaaat gtgatgatat cggtatacgt atcatgattg agatattgtt tattcatctc
      481 aagcagatat tcgtcgctgc aaaaaataaa tgtgagatta cccggaaatt tatggtgcat
      541 cgccattaaa tgttcgagcc aaatagtaat gcgctctttt tcttttaagc gaaataatgt
      601 ctttgtagaa gtatattgaa ttccgagcat atttatttca cgcagaggcg cagagacgca
      661 gaggttagat tttaaattga atttccacac tcgacccgtc attactgaaa atcaccacat
      721 cactcaggtg cttcatcagg aagataccgc gaccaccaac ttcttcgata tgttccggac
      781 tggtgggatc ggggagattg ttgtagtcga atccgcagcc ctgatcttgc acgcagcagc
      841 atagggtatt ttgttcggtg cgaatctgca cttttacctc ttttgtggca tcgctcttgt
      901 tgccgtggag aatagcgttg ttcacggctt cggtgatgca gaccagcata ttgccataca
      961 cctcatccgc caaatgcagg cgctgacgca actcctccac aaatggttcg atgagcgtaa
     1021 tgttaagtgg ttccgatctg aggctgagct gtttcgataa gggttgttcc gacatgctaa
     1081 gcgcgggttt attgattgat gtcccgcaaa tattcttcta ctagattttt gtaaaaaggt
     1141 ttcaaatccg gtgaaacagt tttataaaga tctaactcag cattgcgttt tttgaggtat
     1201 tcctcaatcg ccggcggtat agtacggttg atttcgttgg cggtattgct ctgtctttcg
     1261 ttgtcctgct tgcgctgacg gtcgctctct tctgcttcaa gcaggcgggt gagaatttct
     1321 tcctggcgtt ttaacgtttc ctgggtgatg cgcttgttca caatgtcttc ctcggtctga
     1381 tccatcttat ctgctatctg ctccaattgc ttggcaagct tgccatcctc cgtattcccg
     1441 ccacccaatt gcttggcaag ttcctgcagg gcctgacgca atgccgcctg acgctgggcc
     1501 atttccgcca actcctgact cataccctga ttgcctcccg gtttcttccc ctgctccatc
     1561 tgacccttca tcttgtttag ctgctcgttc agctgcttct gcatctcgct catactcggc
     1621 aattgcgagt cgctgctgcc accgggtttc tgacacatct gactgccggg catgctcttg
     1681 gccatctgtt cctgcatctg ttgcatcacc tcatcaagca tcagggcgag gttgttgtaa
     1741 cccgtcatca cctgctgctg gtagagattg ctcttcgcca cctcgcgatc gcccatttgc
     1801 tctacagttt ctgcaaggcc gtcatttaca gccgataatt cgcgggtgat gaagctgctg
     1861 atctggaata tgcgcttggc gagggcattg aggctgtcct ccaccatttt ggagtcttct
     1921 ttaattttct gctgttgcgc catcagatcc acgaacttag ggttcgacga tttcatgccc
     1981 ttcacctcct tcatcaaatc ctcttgttcc acggaaagcg ttaccaggtt gtcgatgagg
     2041 cggcgcaggg cgttcatgtc ttcctgttgc tgttccatac ccgactgctg catggcgttt
     2101 tgcatctcct tcgccatctc ct
//
LOCUS       DAOQHY010000321         2108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_40270_length_2108_cov_3.941062, whole genome shotgun sequence.
ACCESSION   DAOQHY010000321 DAOQHY010000000
VERSION     DAOQHY010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40270_length_2108_cov_3.941062"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="PLL28_15070"
     CDS             complement(<1..861)
                     /locus_tag="PLL28_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70701.1"
                     /translation="MNNNRLLYHLIGSLSKAEKRYVSRYAAFNRDKSRLLKLFNFINE
                     HGITSDEDLRKSNKNATLLRDIEVSKVHLTQLILVSLRQYHRQEGGERRLRGLMDDAD
                     ILWRKSQYQACAQTLKKVKRLAKSLGYYTYVLEAAYMERNMMGTHPAFPLTEETVDIA
                     NREEKEALEALQTEFKFRNLAARSAIIHKRFGNSRSETDFAEITALAAMPEYDVSEAT
                     TLRSKRFYHHSMLTYYSSIGNLERAVMHNRAILDTYSTHELIRENAAGFIIAHNNQAL
                     LHLRLKQYKSA"
     gene            1021..>2108
                     /locus_tag="PLL28_15075"
     CDS             1021..>2108
                     /locus_tag="PLL28_15075"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNF70702.1"
                     /translation="MTPIKSFVISMFLVGAAWLSAAPAYGQFSLLEIAEGPMASIGSA
                     AFGIESADLDGDGKPDIVSSFYGSGNVNVFRNISTGTGPMQTIFDAPIDITYGTGAAY
                     NVAIADLNKDYKPEILIANTALAEVTILPNHSTPGVINFGTAVHVACPETPYALTACD
                     LNMDGYSDLVATCNSKLAIYRNATAAVGGDITFDPHTELVDATNWRDVMCADMDGDAK
                     VDIVAGGSDRVAIFQSTTPTGGTTLSFAAPVTSITARNTYTMDIGDLDGDFQPDIVTA
                     NWPSPDFTLIRNATTPGTLSLAAPQYITAVSPPGIALGDFDSDGKLDVITMVDGMGWN
                     LAEAFRNTTATPGVITFAAPQTFTPVSD"
BASE COUNT          544 a          526 c          457 g          581 t
ORIGIN      
        1 tgctgatttg tattgtttga gcctcaagtg caataatgcc tgattgttat gcgcgatgat
       61 aaatcctgct gcattttctc gaatcaattc atgcgtggaa taggtatcaa gaatagccct
      121 attgtgcatc actgcgcgct caagattgcc gatgctggaa taataggtga gcatgctatg
      181 atgataaaag cgtttggagc gaagggtagt agcctccgaa acatcatact cgggcattgc
      241 ggcaagtgcg gtaatctccg caaaatctgt ctcacttcgc gagtttccaa agcgcttatg
      301 tatgatagcc gaacgcgcag caagattccg gaatttaaat tctgtttgga gtgcctccag
      361 cgcttctttt tcttctctat tggcaatatc aacagtttct tcagtcaaag gaaacgccgg
      421 atgtgtgccc atcatatttc gctccatgta agccgcttcg agcacatagg tataataccc
      481 taaacttttt gcaagtcgct ttaccttttt gagtgtttgt gcgcatgcct gatattgcga
      541 tttccgccat aaaatatctg catcatccat caacccgcgc agccgacgct cgccaccttc
      601 ctgccggtga tactggcgca acgataccaa tatcaactgc gtcagatgca ccttcgaaac
      661 ctctatatcg cgcagcaagg ttgcattttt attcgatttc ctgaggtctt catcactggt
      721 aatgccatgt tcattgatga agttgaaaag cttcaacagg cggcttttat ctctgttaaa
      781 tgccgcatac cgactcacat atctcttttc agccttgctc aagctgccta tgagatggta
      841 aaggagtcga ttgttattca tagaaactta atcttgaact atcaaaaata gttaaaatta
      901 gtgtataata aatattacaa tgcaccacag gtacttaata tctgtgtaca tttttgcttg
      961 cctgaacctg cctgatagcc gaattttgaa gcatcaagca gaaaacaaaa gcataatcca
     1021 atgaccccaa tcaaaagctt cgtgataagc atgttcctgg taggagcggc ctggctgtct
     1081 gccgctcccg cctacggaca attttcctta ctggaaatag cggaaggccc tatggcttca
     1141 ataggctctg cagccttcgg aattgagagc gctgatttgg atggcgatgg aaagcctgat
     1201 atcgttagtt cgttttatgg ctccggaaac gtcaatgtct ttcgcaatat cagcacagga
     1261 acaggcccta tgcaaaccat attcgatgca cccatcgata tcacttatgg cacgggggct
     1321 gcttataatg tggctattgc cgatctcaac aaagattata aacctgaaat tctcatcgca
     1381 aatacagccc ttgctgaagt aacaattttg cctaatcatt ccactcccgg cgtgataaac
     1441 ttcggaactg ccgtgcatgt ggcatgtcct gaaacacctt acgccctcac tgcctgtgat
     1501 ctgaacatgg atggatacag cgacctggtg gctacttgca attcgaaatt ggcaatttat
     1561 cgcaatgcta cggccgctgt tggtggcgat atcactttcg acccccatac cgaactcgtc
     1621 gatgccacta actggcgcga tgtgatgtgt gccgatatgg acggcgatgc gaaagtggat
     1681 atcgttgccg gtggatctga tcgcgtggca atttttcaaa gtacaacacc tacaggcggt
     1741 acaaccttaa gcttcgccgc tcctgttaca tctataactg ccagaaacac ttatacgatg
     1801 gatatcggcg atttggatgg cgatttccaa ccggatatcg tcactgccaa ctggccttca
     1861 cccgatttta ctcttatccg caatgccaca accccgggaa ccttatcctt ggcagctcca
     1921 caatacatca ccgccgtttc ccctccgggt atcgcactgg gcgatttcga ttcagatggc
     1981 aaattggatg tgatcactat ggtggatggt atggggtgga acctcgcaga agctttcaga
     2041 aacactacag caacacccgg cgtcatcaca ttcgccgctc cgcaaacctt tacacctgtc
     2101 agcgataa
//
LOCUS       DAOQHY010000322         2099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_40566_length_2099_cov_5.647750, whole genome shotgun sequence.
ACCESSION   DAOQHY010000322 DAOQHY010000000
VERSION     DAOQHY010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40566_length_2099_cov_5.647750"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PLL28_15080"
     CDS             complement(<1..350)
                     /locus_tag="PLL28_15080"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNF70703.1"
                     /translation="MFNVRVYGLWIHDGNILIVEEPVRGQMIRKFPGGGLEFGEGIIE
                     CLRREFREELSMEISDIHHFYTTDFFVQSMIDPSHQVISIYYTVSGDPALAIFDKEEQ
                     LQFKWMPINDLHPD"
     gene            complement(343..825)
                     /locus_tag="PLL28_15085"
     CDS             complement(343..825)
                     /locus_tag="PLL28_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNF70704.1"
                     /translation="MKIENGQQAPDFLMYDTDKQPVRLSALKGENVLLLFFPFAFTST
                     CTAELCATRDDIAFYNNMNAKVFGISVDAPYSLKVFREEQQLNFALLSDFNKTVSETY
                     GCIYDRWGMELKGVSKRSAFIIDKEGIVRYAEILENAGELPNFEAIRSTLEQLNAQHV
                     "
     gene            1140..2054
                     /locus_tag="PLL28_15090"
     CDS             1140..2054
                     /locus_tag="PLL28_15090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70705.1"
                     /translation="MKIRLLLTVAALGVATMGFSQVPNGNMDSWDTYTSLASGLSCDF
                     PTQWDVPDKIAADLGIADRNCTKETANVHGGAAAARLETKTLNVLGTPLDVPGTITTG
                     IIGFDFVTFTPSVEGGASVSTNYTALTGYYQYAPSGSDTMNVIVLMMSGADTIGMGQY
                     RDAATSTSYQMFDCPITYFGLGTADKMQITITSSGGFTSLVPGSVLFVDDLAVTGGAD
                     VEDWNGYGIAHNIYPNPASDYINILNPATNDVTIEIYSLTGQKVGEQTLAPDMNSINL
                     ENYTSGIYTFRLVDNGTVVYSSKFVVKK"
BASE COUNT          596 a          445 c          431 g          627 t
ORIGIN      
        1 aaatcaggat gcaaatcatt tataggcatc catttaaatt gcagctgttc ttctttgtca
       61 aatatggcaa gagcaggatc tcctgaaacg gtataataaa tacttattac ctgatgcgaa
      121 ggatctatca tactttggac aaaaaaatca gtggtataaa aatgatgtat atcgctgatt
      181 tccatcgaca actcctcacg aaattctcgt cgcaggcatt caatgatccc ttcaccaaat
      241 tcgagtccgc cacccggaaa ttttctgatc atttgaccac gcaccggttc ttccacgatt
      301 agaatatttc catcgtgaat ccagagacca tagacgcgaa cattaaacat gttgggcatt
      361 caattgctcg agcgttgagc gtattgcttc gaaatttggc agctctcccg cattttcaag
      421 tatttctgca taccgtacta taccttcttt gtcgatgatg aatgcgcttc gtttggaaac
      481 acctttcaat tccattcccc aacgatcgta gatacagcca tatgtttcag aaacggtttt
      541 gttgaagtcg ctaagcaagg cgaaatttaa ttgttgctcc tcgcgaaaca cttttaaact
      601 ataaggtgca tccacgctga tgccgaatac tttcgcgttc atgttattgt aaaatgcaat
      661 gtcatcgcgt gtagcacata actcagcagt gcaagtactg gtaaatgcaa acgggaaaaa
      721 cagaagtaat acattttcac cttttagggc cgacaatcgc acgggttgct tgtcagtgtc
      781 atacattaaa aaatccgggg cttgttgtcc gttctcgatc ttcatctgtg ttaaaaattt
      841 aaagtaaaga tatgctaaac aggggctata acatctttac atgaaaaggg ttcaataaag
      901 tgcattgaac tgtgcgatgc gatatctgcc tgaagtatat gccccttgcc atgccgaagc
      961 cgacagataa aggcttgatt tccatggcag taggggattg tgcggaggct attcgatgtg
     1021 gtcttttggg cgtcaaagaa gctttttgac cgatttgtga aagaaatatc tccacaggtt
     1081 agggaaatcc tgattatatt tatctttgaa acacaattca aataactaaa aacttacgta
     1141 tgaagattcg tttactttta actgttgccg ctctcggcgt agctaccatg ggtttcagcc
     1201 aggtgccaaa tggcaatatg gactcttggg atacctatac ttctctagct tcaggactat
     1261 cttgcgactt cccgacacaa tgggatgtgc cggataagat tgctgccgat cttggtatcg
     1321 ccgatcgcaa ctgtaccaaa gaaactgcaa acgtgcatgg cggcgctgct gcagctcgtt
     1381 tggagacaaa aacattgaac gtattgggta ctccattgga tgttcccgga actatcacaa
     1441 ccggtatcat cggttttgat ttcgttacgt ttacgccttc agttgaaggt ggtgcttcag
     1501 tttctactaa ttacacagct cttacaggtt actatcaata tgccccatca ggcagcgata
     1561 ccatgaatgt tatcgtgttg atgatgagtg gtgctgatac catcggtatg ggtcaatatc
     1621 gcgatgctgc aacgtcaact tcttatcaga tgttcgattg ccctatcact tacttcggac
     1681 ttggaactgc cgataaaatg caaatcacca tcacttcatc aggtggtttc acttcattgg
     1741 ttcccggttc tgtattgttt gtagatgatt tggctgttac aggcggtgct gacgttgaag
     1801 attggaatgg ttatggtatt gctcataata tctatccaaa ccctgccagc gattatatca
     1861 atatccttaa tcctgcaaca aacgatgtaa ctatcgaaat ctatagtctt accggtcaga
     1921 aagtaggtga acaaacactt gctcctgaca tgaacagcat caaccttgaa aactatacaa
     1981 gcggtattta taccttccgt ttggttgaca atggtactgt tgtttatagc agcaaatttg
     2041 ttgtgaagaa ataatcacat aaacatatta caaaaaagta gccccttcaa attgaaggg
//
LOCUS       DAOQHY010000323         2099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_40585_length_2099_cov_3.265166, whole genome shotgun sequence.
ACCESSION   DAOQHY010000323 DAOQHY010000000
VERSION     DAOQHY010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40585_length_2099_cov_3.265166"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PLL28_15095"
     CDS             complement(<1..510)
                     /locus_tag="PLL28_15095"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNF70706.1"
                     /translation="MEFPVDRYRLDTLGCNDTIHFNNAGCSMHPMQVVESICNYLMEE
                     ALTGGYELAHQRASLLNDFYNLGAEMLHAAPNNIAFVSNATDGFAKALSSIPWHTGDV
                     ILTTINDYVSSQIQFASLHKRFGVEVVRAANTPQGDVDIDSIEALIKQYHPRAISITH
                     IPNNTGMIQP"
     gene            complement(513..1322)
                     /locus_tag="PLL28_15100"
     CDS             complement(513..1322)
                     /locus_tag="PLL28_15100"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNF70707.1"
                     /translation="MSKSNTPSNKSKAAKSGSTAKPKKAAEKPEKKAAKKPVATVKEA
                     PKKTVKPVPVAKTKPEGGRIVRHVAIAGNIGAGKTTLTHLLAKHFGWETHFEDVENNP
                     YLYDFYQDMPRWAFNLQIYFLNSRFKQILDIQKGDKTIIQDRTIYEDAQIFAPNLHAM
                     GLMSTRDFQNYTEIFNSITSLVSPPDLLIYLRASIPALVNQIQKRGREYEDNLRLDYL
                     RRLNEYYEAWISKYTHGKLLVIDIDQCNFAENPEDLGDVIQRVQTELYGLF"
     gene            complement(1379..>2099)
                     /gene="trpS"
                     /locus_tag="PLL28_15105"
     CDS             complement(1379..>2099)
                     /gene="trpS"
                     /locus_tag="PLL28_15105"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNF70708.1"
                     /translation="LNMLAYKGELEKVATFKEKIKLHNENINAGLLTYPVLMAADILI
                     HRAYMVPVGKDQEQHLEMTRDYAKRFNNRYDVDFFPEPIAYNFGEELVKVPGLDGSTK
                     MSKTGGETNAIFLNEEPDMIRKKIMRAKTDSGPTEMNSKPSQEIQNLFELMQIVSAED
                     VVSHYKNAWDTCTIRYGDMKKQLAEDMVNYLSPFREKINALMQDTDYLGKIVRRGGEQ
                     ARESAEKTVSEVRKIMGINYF"
BASE COUNT          560 a          441 c          415 g          683 t
ORIGIN      
        1 cggctgaatc atgccggtat tgtttggaat atgtgttatt gaaatcgcac ggggatgata
       61 ttgtttaatt aatgcttcta tgctatcaat atctacatca ccctgtggcg tatttgcagc
      121 gcgaactact tcaacaccaa atcgtttgtg cagtgaggcg aattgaatct gactgcttac
      181 ataatcatta atagtagtaa gaattacatc accggtatgc caaggtattg aggaaagcgc
      241 ttttgcaaat ccgtcggttg cgtttgaaac gaaggcaata ttattaggcg ccgcatggag
      301 catttctgca cctaggttgt aaaaatcgtt caatagtgaa gcgcgttgat gtgctaattc
      361 atagcctccc gtcaatgcct cctccatgag gtaattacat atcgattcaa caacctgcat
      421 cggatgcatg gagcaacccg cgttattaaa atggatggta tcattacagc cgagtgtatc
      481 aaggcgatat ctgtcgacag gaaactccat gatcagaaca atccgtaaag ttcggtttgc
      541 actcgctgaa ttacatcacc gaggtcttcg ggattttcag caaaattgca ttggtcgata
      601 tcgatcacaa gcaattttcc atgtgtgtat ttgctgatcc aggcttcgta atattcgttt
      661 aatcttctca gataatccag gcgaagatta tcttcatatt cgcgaccgcg tttttgaatt
      721 tggttaacca gtgccggaat cgacgcgcga aggtaaatca gcagatcggg tggcgatact
      781 aaagaagtga tggaattaaa tatttcagta taattctgga aatctctggt gctcattaaa
      841 cccatcgcat gcaagttagg agcaaatatt tgtgcatcct cataaatggt gcgatcctga
      901 ataatggttt tatctccttt ttgaatatcc agtatttgtt tgaaacggct attcaaaaag
      961 tatatctgca aattaaatgc ccaacgaggc atatcttgat aaaaatcgta gaggtatgga
     1021 ttgttttcta catcttcgaa atgtgtctcc catccaaaat gttttgcaag taaatgcgta
     1081 agtgtggttt ttcccgcacc aatatttcct gcaatggcaa catgacgaac aatccgacca
     1141 ccttcaggtt ttgtttttgc taccggaaca ggtttcactg tttttttagg cgcttcttta
     1201 acagttgcta caggcttttt agctgctttc ttttccggtt tttcggcggc cttttttggc
     1261 ttagctgtag aaccggactt cgcggccttt gatttattgg aaggggtatt ggacttagac
     1321 atgataggtt tctgatgatt cgggatgagc acttgtatca gcactcacac ataattggtt
     1381 aaaagtaatt aattcccatg atttttcgca cctctgacac tgttttttca gcactttcac
     1441 gcgcttgttc acctcctcga cgcacaatct tacctaaata atcggtatcc tgcatcaatg
     1501 cgttgatctt ttctctgaaa gggctcaaat aattcaccat atcctcagcc agttgcttct
     1561 tcatatcacc atatcgaatg gtgcaggtat cccatgcatt tttgtaatgt gaaaccacgt
     1621 cttcggcaga aacgatctgc atcaactcaa ataaattctg aatttcctgc gatggcttgc
     1681 tattcatttc tgttgggcct gaatcagttt tagcacgcat gatttttttg cgaatcatat
     1741 caggttcttc attcagaaaa atagcatttg tttcgccacc cgttttgctc atttttgtac
     1801 tgccatccaa tcccggtact ttaactaatt cttctccgaa attgtatgca atgggttcag
     1861 gaaagaaatc gacatcatag cgattattaa atcgtttggc ataatcgcgg gtcatttcaa
     1921 gatgctgttc ctgatctttt cccaccggta ccatataagc acgatgaatt aatatatcag
     1981 ctgccatcag cacaggataa gtcaataatc ctgcattgat attttcattg tgcagtttaa
     2041 ttttctcctt aaaggtggct accttctcaa gttcaccttt ataagcgagc atgttcaac
//
LOCUS       DAOQHY010000324         2082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_41141_length_2082_cov_5.212136, whole genome shotgun sequence.
ACCESSION   DAOQHY010000324 DAOQHY010000000
VERSION     DAOQHY010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41141_length_2082_cov_5.212136"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..1110)
                     /gene="mdh"
                     /locus_tag="PLL28_15110"
     CDS             complement(169..1110)
                     /gene="mdh"
                     /locus_tag="PLL28_15110"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNF70709.1"
                     /translation="MSKVTVIGAGNVGSTVANVLAHRDFLNEIIVLDIKEGTAEGKSL
                     DSWQQAPIDNYSTRVTGVTNDYARTADSDVVVITSGLPRKPGMSRDDLISVNAGIVKS
                     VTEQVVKYSPKAIIIVVSNPLDVMTYCAYLTAKIDSSRVFGMAGILDTARYRAFLAEA
                     LNVSPKDIQALLLGGHGDTMVPLPRYTTVSGIPVTELIDMDKLNAIVQRTKVGGGEIV
                     NLLGTSAWYAPGAAAAQMVEAIIKNEQRIFPVCAWLNGEYGLKDIYLGVPCKLGRNGI
                     EEIIQLKLDASEMQLLNDSAKAVREVMDVLDRMNAAN"
     gene            1255..>2082
                     /locus_tag="PLL28_15115"
     CDS             1255..>2082
                     /locus_tag="PLL28_15115"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HNF70710.1"
                     /translation="MKKLLLIAAVGLLGGVLRAQTAPSGYCGTLQTESDLTWLRNYQA
                     TYVPGELERGGTTYYIPTKVHIVGTDDGSGYYNLSYLYQSFCELNEHYAETGFIFYIY
                     GDLNYVDNTDYYEHDWFDGSNMMEDYNVNNLLNVYFVSDPAGNCGYFSPSDDGVAVAK
                     SCAMPGGTTIAHEFGHFFSLPHTFYGWEWGTPDDNDQEWVNGANCHSAADGFCDTPPD
                     YLPYRWNCPGPLQTDPHGETFYSDGTFYMSYSNDACTDKFSAEQQDAMISNLLGPRNN
                     "
BASE COUNT          591 a          463 c          438 g          590 t
ORIGIN      
        1 aaagcgaata gtttttcgtt gaatttttcc gtgaataaat atgtgaatcc ctttgggagg
       61 tatctcttcg agaataagtg ggatttgtgc gatctctatc attgattaaa aataaaaaaa
      121 ccgcaaagca attcaagagc tctgcggttt tcaaaaatat tgattcaatt agtttgcagc
      181 attcatgcga tcaagcacat ccattacttc acggactgct tttgcagaat cgttcaataa
      241 ttgcatttct gatgcatcca atttcaattg aataatttct tcaatgccat tgcggccaag
      301 cttacaaggt actccaaggt agatatcttt aaggccgtat tcaccattca accatgcaca
      361 aacagggaat atgcgctgtt cattttttat gatcgcctcc accatttggg cagcagctgc
      421 tcccggagca taccatgctg aagttccaag taaattcaca atctcgccac caccaacttt
      481 tgtgcgttga acaatagcat tcagtttatc catgtcgata agctctgtta caggaatacc
      541 gctaacggta gtgtaacgtg gaagcggaac catagtgtcg ccatgaccac ccaacaacaa
      601 agcttgaata tctttaggag aaacgtttaa agcctcagca aggaaagcgc gatagcgggc
      661 agtatccaga atacctgcca taccaaaaac gcggctgctg tcaattttgg cagtaagata
      721 tgcacaatag gtcatcacgt ctaatgggtt agaaaccaca atgatgatgg cctttggtga
      781 gtattttact acttgctctg tcacagattt aacaatgccg gcgttaacac taattagatc
      841 atcgcggctc ataccgggtt ttcttggtaa tccggaagtt atcactacca catctgaatc
      901 tgcagtgcgc gcgtaatcat tggtaacacc cgtaacacgc gtgctatagt tgtcgattgg
      961 cgcctgttgc catgaatcaa ggctctttcc ttcggcagtg ccttctttga tatcaagaac
     1021 gatgatttcg ttcaaaaaat cacggtgagc gaggacgttt gccacagtgg aaccaacatt
     1081 gcccgctccg ataacagtta ctttactcat agttgtgtca ttttagctac taaaactgga
     1141 cttgcggctg caaaattagg tgttttacag cgcacgtaag gtattgtgca caaactttgc
     1201 tgcaatattt ctgttacctt tttaacttta cacatcaatt ccacatccat agttatgaag
     1261 aaattacttt tgattgctgc agttggcttg ctgggaggcg ttttgcgcgc tcaaacagct
     1321 ccttccggat actgcggtac actacaaaca gaatcagatc ttacctggct gcgaaattac
     1381 caggctacct acgttccggg tgaactggaa cgcggcggta caacctatta cattcctact
     1441 aaagtgcata ttgtaggaac agatgatggc agtggctatt acaatctgag ctacctatat
     1501 cagtcatttt gcgaattgaa cgagcactat gccgaaacag gattcatctt ctatatctat
     1561 ggagatttga actatgttga taacaccgat tactacgaac acgattggtt cgatgggtcg
     1621 aatatgatgg aagattacaa tgtaaacaac ttactgaatg tttacttcgt atctgaccct
     1681 gcaggaaact gtggctattt cagcccttca gatgatgggg tagctgtagc taaatcatgt
     1741 gcaatgccgg gtggaactac catcgcgcac gaatttggtc actttttctc attgccgcat
     1801 accttttacg gttgggagtg gggaacaccg gacgataatg atcaggaatg ggtaaacggt
     1861 gcaaactgtc attcagctgc cgacggattt tgcgatacac caccggatta cctcccttac
     1921 cgctggaatt gccccggacc actgcaaacg gatccacatg gcgaaacttt ctatagcgat
     1981 ggaacattct atatgtcgta ttcaaatgat gcttgtaccg acaaattttc tgcagaacaa
     2041 caagatgcga tgatcagtaa cctgttggga ccaagaaata ac
//
LOCUS       DAOQHY010000325         2080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_41223_length_2080_cov_4.922963, whole genome shotgun sequence.
ACCESSION   DAOQHY010000325 DAOQHY010000000
VERSION     DAOQHY010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41223_length_2080_cov_4.922963"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1968)
                     /gene="lon"
                     /locus_tag="PLL28_15120"
     CDS             complement(<1..1968)
                     /gene="lon"
                     /locus_tag="PLL28_15120"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNF70711.1"
                     /translation="MRDYGFDDQAFSSMVEDEVEFLPILSLEEDEDNTKIEYPETIVV
                     MPLRNTVLFPGIVLPITVGREKSIKAIQNAFRSNKLIGVVAQKDGNIEDPETNQLYTT
                     GVIAKVIKQLKMPDGSTTVIIQGKKRFVINEFTTKEPYLQAKISLLEDKPHPDQHEFE
                     AQISAIRDLANSIVKVSPNIPQEASVVLRNINSPIYLLHFVSSNLQIEVADKQKILEA
                     DNLQSRAQLVLNHLTSELQLLELKNKIQNKVRNDMDKQQREYFLNQQLRTIQEELGGS
                     GQDEEIKRYRQRAEDKKWAKHVQEVFDKEIEKLQRLNPNAAEYGVITNYIELLLELPW
                     NEYTTDNFDLKHAQDVLDQDHYSLEKVKDRLIEYLSVLKLKGDLKSPIICLVGPPGVG
                     KTSLGQSVARALGRKYVRMSLGGLHDESEIRGHRKTYIGAMPGRVLQSIKRVKSSNPV
                     FILDEIDKVGTDFRGDPSSALLEVLDPEQNSTFYDNYLEIEYDLSKVLFIATANNINS
                     IQPALRDRMEIIYLSGYSTEEKIEIAKRHLLPKQKSEHGLKPKDIKISDAGLLTIVEN
                     YTRESGVRDLDRQIASVMRRIAKYVATNEAYAKTLQPADIIKILGPEKFDKESIFDNM
                     PAGVVVGLAWTPVGGDVLFIETSLSKGKGTLQ"
BASE COUNT          532 a          485 c          391 g          672 t
ORIGIN      
        1 ctgcaaagtg cccttaccct tgctgagcga tgtttcaata aataatacat caccaccaac
       61 aggtgtccat gccagtccta caactacacc ggcaggcata ttatcgaaga tactttcctt
      121 gtcgaatttt tcgggcccta aaattttaat gatatcggca ggttgcaatg tttttgcata
      181 tgcttcattt gtagccacat attttgcaat gcgacgcatc acggaagcaa tctgcctgtc
      241 gagatcacgc acaccgcttt cacgtgtata attttctaca atggtcaaca aaccggcatc
      301 agatatttta atatcctttg gttttaatcc gtgttcgctt ttttgcttcg gcaacaaatg
      361 tcgcttggca atttctattt tctcttcggt agaataaccg ctgagataaa taatctccat
      421 cctatcgcgc aaggcaggtt gtatgctgtt gatgttattc gcggtagcaa taaataatac
      481 cttggaaagg tcgtattcta tttccagata attatcgtaa aaggtgctgt tttgttccgg
      541 atctagtact tctaataatg cactggatgg atcgccacga aaatctgtgc ctactttatc
      601 tatttcatca agaatgaaaa cagggttcga tgattttacg cgtttgatag actgcaatac
      661 acgtcccggc atggcaccga tgtatgtttt tcgatgaccg cgtatctcgc tttcatcatg
      721 caatccaccc aaactcatgc gcacatattt tctgcccagg gcacgcgcta ccgactgtcc
      781 caatgaagtt tttcctaccc ccggaggccc aaccaaacaa atgatagggg atttcagatc
      841 acctttcagt tttaaaacag aaagatattc tattaatcta tccttaacct tttccagact
      901 ataatggtcc tgatcgagca catcttgcgc atgtttcaga tcaaagttat cggtggtata
      961 ttcattccac ggcagctcaa gcaataattc aatataattg gtgatgacgc catattcagc
     1021 ggcattggga tttaagcgct gcagtttttc aatttcttta tcgaaaactt cctgtacatg
     1081 ctttgcccat tttttatctt ccgcgcgctg gcgatagcgt ttaatttctt catcctgacc
     1141 tgaaccgcca agttcttcct gtatggtacg caactgctga ttgaggaaat attcgcgttg
     1201 ctgcttatcc atatcattgc gcaccttatt ctgaatcttg ttttttaatt ccagaagctg
     1261 cagctcactc gtcaaatgat ttaataccaa ttgtgcacgc gattgcaaat tatcggcttc
     1321 gagaattttt tgcttgtcgg caacttcaat ctgaagattt gaagaaacaa aatgcaagag
     1381 atatatcgga ctgttaatat tgcgcaatac tacactggcc tcctgcggaa tatttggcga
     1441 tactttcacg atactgtttg ccagatcgcg aatagcactg atttgtgctt caaattcatg
     1501 ttgatcggga tgcggtttgt cttccagcaa actgatcttg gcctgcaggt aaggttcttt
     1561 ggtagtgaat tcgttaatga caaatcgctt ttttccctga atgataacag ttgtgctgcc
     1621 atccggcatt ttcaattgct tgatcacctt cgcgataaca cccgtagtat acaattgatt
     1681 tgtctccggg tcctcgatat tgccatcctt ttgtgcaact acaccgatta acttattgct
     1741 gcgaaaagca ttttgtatgg ccttaataga tttctctctg ccaacagtta taggtaacac
     1801 tattcctgga aacaaaacgg tattgcgcaa gggcatcacc actatcgttt cagggtattc
     1861 tattttcgta ttgtcttcgt cctcttcaag cgacagtatg ggtagaaatt ctacttcatc
     1921 ttccaccatc gacgaaaagg cctgatcatc aaatccgtaa tctctcatat gcgatctttt
     1981 gtttattcta ggcagtctcc gaccaccccg cttcctgtgt tcaattgttg tgcctgtcct
     2041 attccgactg ccagaatacg cgaaatctgc caaaagccgt
//
LOCUS       DAOQHY010000326         2074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_41418_length_2074_cov_4.745914, whole genome shotgun sequence.
ACCESSION   DAOQHY010000326 DAOQHY010000000
VERSION     DAOQHY010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41418_length_2074_cov_4.745914"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..526
                     /locus_tag="PLL28_15125"
     CDS             59..526
                     /locus_tag="PLL28_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HNF70712.1"
                     /translation="MSTPQQILDNVKQFIQSVGVDPATCWNEQNKAYYVYKGSAKLEI
                     FVSSHPQNDGTTRHYLRIFSGLIKVPATNKEYFYRRCMEISDQSLGVKLTVMSNAAPE
                     NDWVYATYERDIAGMDYNETVTCVNDMGLWADWLDDQLKQEFGGGAGPGAPQQ"
     gene            complement(593..1543)
                     /locus_tag="PLL28_15130"
     CDS             complement(593..1543)
                     /locus_tag="PLL28_15130"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNF70713.1"
                     /translation="MSNLSVNHLISIKDLQPEDIHLILQTADNFKEVLNRPIKKVPTL
                     RDITIANIFFENSTRTRISFELAEKRLSADIINFSASASSVKKGETLIDTVNNILAMK
                     VDMVVMRHQSPGAPVLLSKHIKANIVNAGDGTHEHPTQALLDAFSMMEKLGESRGERL
                     KDLKGVRVAIIGDILHSRVAGSNIHALKMLGAEVTLCGPPTLMPRYIETLGVHVSHNV
                     KETLEWCDVANVLRIQLERQDIKFFPSLREYSLYYGIDKAMLDSINKDIVIMHPGPIN
                     RGVELSSDVADSKQSIILQQVENGVAIRMAVLYLLAGKLD"
     gene            complement(1545..2045)
                     /gene="pyrR"
                     /locus_tag="PLL28_15135"
     CDS             complement(1545..2045)
                     /gene="pyrR"
                     /locus_tag="PLL28_15135"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNF70714.1"
                     /translation="MERLCHQLIENHGDFSNACIIGVQPRGVNLAKRIVWKLEELLPG
                     TKIKFGILDTTFYRDDFRHRDKPLLAEETDIDFVIKGKEVILVDDVFYTGRTIRSALD
                     ALLDFGRPEKIELLVLIDRRLSRHVPITPDYVGKTIDAVTSEKVRVEWSENGIDDRVW
                     IVPHKD"
BASE COUNT          585 a          479 c          390 g          620 t
ORIGIN      
        1 tcctctttcc tctctccttt tccttatctt agcccttcat caactaaact caacaatcat
       61 gtccaccccc caacaaattc tggataatgt gaaacaattt atccaatcag taggtgtcga
      121 tcctgcaacc tgctggaatg agcaaaacaa agcgtactat gtgtacaaag gatctgcaaa
      181 acttgaaata tttgtgagca gtcacccaca aaatgatggc acaacacgtc actatctgcg
      241 cattttcagc gggttgatta aagttcctgc aaccaacaaa gaatatttct accgtcgatg
      301 catggaaatc agcgatcaaa gccttggtgt taagctcacc gtaatgtcaa atgctgcacc
      361 ggaaaacgat tgggtatacg ctacctatga acgcgatatc gccggtatgg attacaatga
      421 aaccgtaacc tgtgtcaacg atatgggctt atgggcagac tggctcgacg atcaattgaa
      481 acaagagttc ggtggaggtg ccggtcccgg tgctcctcag caataaattt ttgtataaaa
      541 aaaatccttg ctcattgcag ggattttttt ttgctattca aatttgaaaa aattagtcca
      601 gtttacctgc caataaataa agcactgcca ttctgatggc aacaccgttt tcaacctgct
      661 gcagaattat tgattgtttg ctatctgcaa catcgctgct cagttccact cccctgttga
      721 ttggccctgg atgcattatt acgatgtctt tattgatact atccaacatc gctttgtcga
      781 tgccatagta caacgaatat tcgcgcaatg aagggaagaa tttaatatcc tgtcgctcta
      841 attgaatgcg caacacatta gctacatcac accattccaa cgtttctttt acattgtgcg
      901 aaacatgtac gccaagcgtt tcaatatatc taggcatcag tgttggaggc ccgcataaag
      961 tgacttcggc acccaacatt ttcagtgcat gaatattgct gcctgcaacg cgtgaatgca
     1021 aaatatcacc aataattgcg acgcgcactc cttttaaatc tttcaatctt tcaccgcgcg
     1081 actcacccaa tttttccatc atactaaatg catcaagcaa tgcttgtgta ggatgttcat
     1141 gcgtgccatc acctgcatta acgatattag ctttaatatg cttgctgagt agaaccggcg
     1201 ctcccggact ttgatgtcgc attaccacca tgtctacttt catggcaaga atattattaa
     1261 cggtatcgat aagtgtttca ccttttttta ccgaagatgc cgatgctgaa aaattaatga
     1321 tatcggcgct caatcgcttt tcagcgagct cgaaagaaat gcgtgtgcgt gtactatttt
     1381 cgaaaaatat gttggcgatg gtaatatcgc gaagtgtcgg aaccttttta atcggtctgt
     1441 tgaggacttc tttaaaatta tctgctgtct ggagaataag atgaatgtcc tccggttgaa
     1501 ggtctttaat ggagatcagg tggttgacac tcaggttact catattaatc tttatgtggt
     1561 actatccaaa cgcgatcatc aatgccgttt tcactccact caacacgcac tttttcgctt
     1621 gtaacggcat ctatagtttt acctacataa tcgggagtga tgggcacatg tctgctcaat
     1681 cgtctgtcta tcagcaccag taattcaatt ttttccggtc ttccgaaatc cagcaatgca
     1741 tccaaggcac tgcgaattgt tcttcctgta taaaaaacat catcaaccaa aatcacttct
     1801 ttacccttta tcacaaaatc gatatctgtt tcttctgcca gcaatggttt atcgcgatga
     1861 cggaaatcat cacgataaaa tgtagtatcc agtatgccga atttaatttt tgttccgggc
     1921 agcaattcct ctaatttcca tacaatgcgc ttggccaaat tcactccacg cggttgcacc
     1981 ccgataatac aggcattaga aaaatctccg tggttttcaa ttaattggtg acaaagccgc
     2041 tcaatggtga gtcggaactt ggaactatcg agaa
//
LOCUS       DAOQHY010000327         2059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_41976_length_2059_cov_22.184631, whole genome shotgun
            sequence.
ACCESSION   DAOQHY010000327 DAOQHY010000000
VERSION     DAOQHY010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41976_length_2059_cov_22.184631"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            438..>2059
                     /locus_tag="PLL28_15140"
     CDS             438..>2059
                     /locus_tag="PLL28_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70715.1"
                     /translation="MMLFGSIALMAQSSECPTVGNPPDMYYNIFQSKSTGEPAPVYVK
                     LYFHVFQEDDGSGGLTASDIETAKSNLNAAYNPHGIYFVYHCDIDFIKNSYLYKYGLD
                     EVNYHLPNSCAVWNTNHQIDGINIYFISPHNYANVAGTFGVAENIPSKNCIIGYAYDG
                     VDGGASAALTSTLVHEVGHCLGMLHTHAGSGNNPKGPCPLKPDYFYDCEGNFHLIEYI
                     AAEDSDNCHTAGDKICDTPPDHYKFGDLPSKCQIENNYPKVFLKDACPGLIECYADQG
                     IGFTAQDWADTLANPFKIEILTPECQKYNPDLTNYMTTGKTRSCRDHFTTQQGMVMKN
                     FINTHPVLASVRRTADDYANATNCPCSSQDIHIYDQQSYAADQIINGNVYIHAGGNLT
                     ITAKIQFPEGKGIIVERGGKLKVTGPNAKLTAACNYQWWNGIEVEGNNQKEQPDGYLS
                     ATTSDDAGIVLVESNATIEKAKVAISTRRHGEYWNEAYWGGLVQLKDCYFKGNRKAVE
                     FMRYRIDLNGTNKSYITNVAFDGETPNSTTSEG"
BASE COUNT          677 a          404 c          405 g          573 t
ORIGIN      
        1 tacacgcatt caattaaaaa ttaaaaatta aaaattaaaa gttataagtg aaaagtgaaa
       61 agtgagaagt gaaaagtgag aagtgaattg caaattttaa tgcttggcat ttacgcatga
      121 tggtattaag agaaagtgca acaactgccc taacatctgt cagaagactt acgaatccaa
      181 cattttttgt aaaataaatg caatattttt ttgaactgat tataaaatta tttacatttg
      241 agccatgcgt tctgaatgat tcggcaacgt cattcttttg ttttttggtt ttcatcatag
      301 atagtatttt ctaacctttc aatattttgt tatgaaaaag ctttacaaac tcagagaaca
      361 aaattattgc atcaattata cattgatggc aaaatttaat tttctctccc ccccccattc
      421 acctattaat tttcttaatg atgctgttcg gtagcattgc tttgatggcc cagtcaagtg
      481 aatgccctac ggtaggcaat ccacccgaca tgtattacaa tatcttccaa agcaaatcaa
      541 ccggcgaacc ggctccggtg tatgtgaagc tttattttca tgtgtttcag gaagacgatg
      601 gcagtggcgg acttacagca tcagatatag aaactgctaa gtcaaacctg aatgctgcat
      661 ataacccaca tggcatatac tttgtttatc attgtgatat tgattttata aagaattcct
      721 atttgtacaa atatggtttg gacgaggtaa attatcatct accaaacagt tgcgctgttt
      781 ggaataccaa ccatcaaatc gatggaataa atatttattt tatcagccct cataattatg
      841 ctaatgttgc aggaaccttt ggggtagcgg agaatatacc atcaaaaaac tgtataattg
      901 ggtatgcgta tgatggagta gatggtggag catctgcagc actaacttca acattggttc
      961 atgaagttgg tcattgcctt ggaatgttac atacacatgc tggatccgga aataatccaa
     1021 aaggcccttg cccattaaaa ccagattatt tttatgattg cgaaggaaat tttcatttaa
     1081 ttgaatatat cgctgctgag gatagtgaca attgtcatac tgcaggcgat aaaatatgcg
     1141 atacacctcc tgaccattat aaattcggtg acctgccatc taaatgccaa attgaaaata
     1201 actatcctaa agtttttcta aaagatgcct gtccaggatt aatcgaatgc tacgctgatc
     1261 aaggaatagg atttaccgcc caagattggg cagatactct tgctaatcca tttaaaatcg
     1321 aaatcttaac tccagagtgc caaaaataca acccggattt aacaaattat atgacaacag
     1381 gtaagacaag aagttgtcgc gatcatttca ctacccaaca aggtatggtt atgaagaatt
     1441 ttatcaacac ccatccggta cttgcatcgg ttaggcgcac ggctgatgac tatgccaatg
     1501 ccaccaactg cccctgcagt agccaggata tacacatata cgaccagcag agctatgctg
     1561 ctgaccaaat aatcaatggc aacgtatata tacatgccgg tggcaatctg acaattactg
     1621 caaaaatcca attccctgaa gggaaaggca tcatcgtaga gagaggtggc aagctcaagg
     1681 tgactggacc taatgccaag ctgacagctg cctgcaacta ccaatggtgg aacggcatag
     1741 aagtggaggg caacaaccag aaagaacaac ctgatggtta tctttcagcc actacaagcg
     1801 atgatgcagg tatcgtattg gttgaaagca atgctaccat agaaaaagcc aaagttgcca
     1861 tcagtaccag acgacatggc gaatactgga atgaagccta ctggggcggc ctcgtgcagc
     1921 ttaaggactg ttacttcaaa ggaaacagaa aggctgtgga atttatgcgt tatcgcatcg
     1981 acctcaacgg caccaataag tcgtacatca ccaatgtagc gtttgatgga gagacgccta
     2041 attctactac atccgaagg
//
LOCUS       DAOQHY010000328         2036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_42828_length_2036_cov_4.423523, whole genome shotgun sequence.
ACCESSION   DAOQHY010000328 DAOQHY010000000
VERSION     DAOQHY010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42828_length_2036_cov_4.423523"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /gene="rimO"
                     /locus_tag="PLL28_15145"
     CDS             complement(<1..1102)
                     /gene="rimO"
                     /locus_tag="PLL28_15145"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNF70716.1"
                     /translation="MKARTLKKDKVNIITLGCSKNTVDSEVLLSQLKANDVQAFHERE
                     KDDCNIIIVNTCGFIDKAKQESIDTILNYAEAKSNGEIEKLYVTGCLSHRYKDELQQE
                     IPEVDAWFGTMELPAILKRFNADYKHELLGERMLSTPAHYAYLKISEGCNRTCSFCAI
                     PLMRGQHVSRTIEDLVKEAQYLASVGVKEIMLIAQELTYYGLDIYKKRALSDLLKELD
                     KVEGIEWIRLHYAYPSKFPLDVIETIRDSKHICRYLDMPLQHASDNMLKAMNRQITLE
                     ETKELITNIRNIIPDITLRTTMLVGFPGETDEDIDALCAFVEWAKFDRLGVFTYSHEE
                     GTSGYALNDDIPAEVKAERAARVMEVQEEISIA"
     gene            complement(1121..>2036)
                     /gene="ftsY"
                     /locus_tag="PLL28_15150"
     CDS             complement(1121..>2036)
                     /gene="ftsY"
                     /locus_tag="PLL28_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNF70717.1"
                     /translation="EDLNKGLEKTKQSLFSKITKAIAGKSRVDDEFLDELEQILISSD
                     VGLDTTIKIIDRLNKRVSRDKYMGADELNAIMKDEIVQMLSENNTIDYTDVVIPQNVK
                     PYIMLIVGVNGVGKTTTIGKLAWQFKQQGKKVLLGAADTFRAAAVDQLTIWSERAEVP
                     IVKHQMGADPAAVAFDTVNAAKSQDADVVLIDTAGRLHNKVNLMNELSKIKRVIQKVY
                     PDAPHDVMLVLDASTGQNALEQAKQFTAATDVTCIALTKLDGTAKGGVVLGIADQFKI
                     PVRYIGVGEKMHQLQVFNKKAFVDALFE"
BASE COUNT          526 a          457 c          409 g          644 t
ORIGIN      
        1 tggcgataga aatctcttcc tgcacctcca tcactctggc tgcgcgttcg gcttttactt
       61 cagcgggaat atcatcgttc aatgcatagc cgcttgttcc ttcttcatgc gaataagtaa
      121 acacccccag gcgatcgaat ttcgcccatt caacaaatgc acacaacgca tcaatatcct
      181 cgtcagtttc tcccgggaat ccaaccaaca ttgttgtacg caaagtaata tcgggaataa
      241 tatttctgat attggtaatt aattctttag tctcttctaa tgtaatctga cgattcattg
      301 ccttcaacat attatctgaa gcatgttgca aaggcatgtc gaggtagcga caaatatgct
      361 tgctgtcgcg aatggtttca atcacatcca atggaaattt tgatggataa gcataatgca
      421 ggcgtatcca ttcaattcct tccaccttat ccaattcttt caacaaatca gaaagcgcac
      481 gttttttata aatatcgagt ccgtaatacg tgagttcctg agcaatgagc atgatctcct
      541 tcaccccaac gcttgcgaga tattgggctt ctttgacaag atcttctatt gtacgggaaa
      601 cgtgttgtcc gcgcatcaat ggtatcgcgc aaaatgaaca agtgcgatta caaccttcag
      661 atattttcag ataggcataa tgtgcaggtg tcgacaacat gcgttcgccc agcaattcgt
      721 gtttataatc ggcattaaag cgtttgagga tggcaggaag ttccatggta ccaaaccatg
      781 catccacctc agggatttct tgttgaagct catctttata tcgatgactg agacaaccgg
      841 ttacatacaa tttttcaatt tcaccattag attttgcttc ggcataattc aagatggtgt
      901 cgatagattc ttgttttgct ttatcaataa atccgcaggt attcacgatg atgatattac
      961 aatcatcttt ctcacgttcg tgaaatgcct gcacgtcgtt ggctttcagt tgcgacaaca
     1021 gcacttcact atcaacggta tttttactac aaccgagtgt aatgatattg accttatctt
     1081 ttttgagtgt tcttgccttc accttataat cattgtgcta tcattcaaat aaggcatcaa
     1141 caaatgcttt tttattgaac acctgcagtt gatgcatttt ttcgccaaca ccgatatagc
     1201 gaacaggaat tttaaattga tcagctatac ctaatacaac gccaccttta gcggtgccat
     1261 cgagtttggt gagggcgata caggttacat cggtagccgc agtaaattgt tttgcttgtt
     1321 cgagcgcatt ttgaccggta gatgcatcga gtacgagcat gacatcgtgc ggtgcatccg
     1381 gatatacttt ctgtattacg cgcttgatct tagaaagttc attcatcagg ttcaccttat
     1441 tatgcaatct tcctgcagta tctatcagca ccacatcagc gtcttgcgat ttcgctgcgt
     1501 ttacggtatc aaatgccact gctgcgggat cggcgcccat ctgatgtttt acgataggca
     1561 cttcagcacg ttccgaccag atagttaatt gatctacggc agctgcgcga aaggtatcgg
     1621 cggcaccgag taatactttt ttaccttgtt gtttaaattg ccatgcaagt tttccgatgg
     1681 tagtggtttt gccaacacca ttcacaccaa ctatgagcat gatataaggt ttcacatttt
     1741 gagggataac gacatcggta taatcgatgg tattgttttc cgacaacatt tgaacgatct
     1801 catctttcat gatcgcattc aattcatcgg cgcccatata tttatcgcgc gacacacgtt
     1861 tattgaggcg gtcgatgatc ttgatggtgg tatcgaggcc aacatcactg gatatgagaa
     1921 tctgttccag ttcatccaaa aattcgtcat ctacgcgcga ttttccggcg atggctttgg
     1981 tgatcttgga gaagaggctc tgttttgttt tttcgagccc tttgttgaga tcctct
//
LOCUS       DAOQHY010000329         1997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_44335_length_1997_cov_5.110711, whole genome shotgun sequence.
ACCESSION   DAOQHY010000329 DAOQHY010000000
VERSION     DAOQHY010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44335_length_1997_cov_5.110711"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PLL28_15155"
     CDS             <1..210
                     /locus_tag="PLL28_15155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70718.1"
                     /translation="PNPANNDIYFSPLTQNALLEVYDMAGKRLLQASAGIGDNHLDIS
                     MLPSGTYLLKMIAQDNMAIAKFIRQ"
     gene            212..1840
                     /locus_tag="PLL28_15160"
     CDS             212..1840
                     /locus_tag="PLL28_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HNF70719.1"
                     /translation="MTTTQSAFAPIFPSWESIPENARLQFPIHQSQYLLNGTLKNWEG
                     NFTKVISPIYVRTGDTLERVVIGEIPHFTVSEAMLALDAAAKAYDLGRGEWPTMSVKK
                     RIECLITFTEKMKTKREEVVRLLMWEIGKSRADSEKEFDRTVLYIYDTIDALKELDRT
                     SSRIQKEEGIYAQIRRGPLGVVLCMGPYNYPLNETFSTLIPALIMGNTVLFKPAKYGV
                     LLINPLQECFRDSFPAGVVNMLYGDGKEIVTPIMESGKVDVFAFIGTSRVANIIRKAH
                     PKPNRLRACLGLEAKNPAIILPDADLEIAATECITGSLSFNGQRCTALKMIFVHEQIA
                     DAFIKLYTEKLGKLKYGMPWDEKVMLTPLPEENKPGYLNDLIADAKEKGASIVNEHGG
                     EQYLSFCYPTVLFPVNENMRVWHEEQFGPVIPIATFSDINEPIAYIQNSNYGQQCAIF
                     GTDHDKMAQLIDPLVNQVCRLNVNSQCQRGPDKYPFNGRKDSAEGTLSVTDALRVFSI
                     RTTVAFKDSGINREIVNDILDNRKSNFLSTDYIL"
BASE COUNT          632 a          422 c          411 g          532 t
ORIGIN      
        1 cccaaccctg caaacaatga tatttatttt tctccgctca cacaaaatgc attattagaa
       61 gtttatgata tggccggaaa gcgccttctg caagcatccg caggcatcgg tgataatcat
      121 ctggatatta gcatgttacc ttcaggtact tatttactca aaatgatcgc acaagacaat
      181 atggcgatcg caaaatttat caggcaataa tatgacaaca acgcagagtg catttgcacc
      241 catcttccct tcatgggaat ctattccgga aaacgcaaga ttacaattcc ccatacatca
      301 atcgcaatat ttattaaacg gcacacttaa aaattgggag ggcaatttta cgaaagtgat
      361 ttcaccaatt tatgtccgca caggcgacac attggaacgt gtcgtaattg gcgagatacc
      421 acattttaca gtgagcgaag ccatgttggc actcgatgcc gccgcaaaag cctatgacct
      481 cggtcgcggt gaatggccta ccatgtcggt gaaaaaacgc atcgaatgcc tgatcacctt
      541 caccgaaaaa atgaagacaa aacgcgagga agttgtacgc ctgctcatgt gggaaatcgg
      601 taaatcacgt gccgattctg aaaaagaatt cgatcgtacg gtattatata tctatgatac
      661 catcgatgcc ctcaaagagc tcgacagaac atcatcgcgc attcaaaaag aagaaggtat
      721 ttatgcgcaa attcgtcgcg gtccgctggg tgtggtattg tgtatgggac catataatta
      781 tcctttgaat gaaacattca gcacattgat tccggcgctc attatgggca ataccgtatt
      841 atttaagcct gcgaaatatg gtgtgttatt aattaatcca ctgcaggaat gttttcgcga
      901 tagcttccct gccggcgttg taaatatgtt gtatggcgat ggaaaggaaa ttgtaacacc
      961 gattatggaa tccggaaaag tagatgtgtt cgcatttatt ggaactagtc gcgttgccaa
     1021 tattattcgc aaggcacatc caaagccaaa tcgcttgcgc gcatgcctcg gactggaagc
     1081 aaaaaatccg gcaattattt taccggatgc cgatctggaa atagcagcaa cagaatgtat
     1141 cacaggctca ctttcattca atggtcagcg atgcacggca ttgaaaatga tattcgtgca
     1201 tgagcaaatt gcagatgcct tcatcaaatt gtataccgaa aaactcggca aattaaaata
     1261 tggaatgcct tgggatgaaa aagtgatgct cacgccactt cccgaagaaa ataaacccgg
     1321 ttatctcaat gatttaatag cagatgcaaa agaaaaagga gcaagtattg tgaatgagca
     1381 tggcggcgaa caatatttaa gtttttgtta tccgacagta ttatttccgg tgaatgaaaa
     1441 tatgcgagta tggcatgaag agcaattcgg accggtaatt cctattgcca ctttcagcga
     1501 tatcaatgag ccgattgctt atattcaaaa cagcaactac ggacaacaat gcgccatttt
     1561 cggcaccgac catgataaga tggcacaatt aattgatccg ctggtaaatc aggtatgccg
     1621 attaaatgtc aactcccaat gtcagcgcgg acccgataaa tatccattta atggaagaaa
     1681 agattccgcc gaaggcacat taagtgtaac cgatgcactg cgcgttttca gcatccgcac
     1741 caccgtagcc ttcaaagact ccggcatcaa ccgtgagatc gtgaatgata ttttggataa
     1801 tagaaaaagt aattttttgt cgacggatta tatattgtaa taactgataa ttaatccata
     1861 agattcaaag attcgaagat ttgaaggaca gaagggaagg tttttaagtt gaacaacatt
     1921 cctattcctt ttttaaccct tcgaatctta gactcttcaa cccttcgaat cttagactct
     1981 tcaacccttc gaatctt
//
LOCUS       DAOQHY010000330         1987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_44754_length_1987_cov_4.480331, whole genome shotgun sequence.
ACCESSION   DAOQHY010000330 DAOQHY010000000
VERSION     DAOQHY010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44754_length_1987_cov_4.480331"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..667)
                     /locus_tag="PLL28_15165"
     CDS             complement(119..667)
                     /locus_tag="PLL28_15165"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNF70720.1"
                     /translation="MKIYTKKGDEGQTSLIGGTRVPKHHLRVEAYGTIDELNVCIGLI
                     LDQQMHEDLRNVLRTIQDRLFTVGAALASDPERSKMSIPDLVDADVKQLEDAMDAMDA
                     ELAPLQNFILPGGHITVSYCHLARVVCRRAERIVTHLAENEHVEPIILIYLNRLSDYL
                     FVAARKIAHDLGIEEVAWRPRG"
     gene            complement(693..1421)
                     /locus_tag="PLL28_15170"
     CDS             complement(693..1421)
                     /locus_tag="PLL28_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNF70721.1"
                     /translation="MSDIITLSNIRKTYDLGKMRIEVLKGLNTTIAKNEYVAIMGPSG
                     SGKSTLMNILGCLDKPTKGQYVLNGTDVSRMNEDQLATVRNEEIGFVFQTFNLLARLS
                     GIENVALPLIYAGIKRNERVERATAILQAVGLGHRMHHKPNELSGGERQRVAVARALV
                     NNPSIILADEPTGNLDTKTSYEIMELFEDIHAKGNTVIIVTHEEDIARYAHRIIRIRD
                     GVIERDEVNANKLNPTELRKNLVS"
     gene            complement(1424..1714)
                     /gene="gatC"
                     /locus_tag="PLL28_15175"
     CDS             complement(1424..1714)
                     /gene="gatC"
                     /locus_tag="PLL28_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNF70722.1"
                     /translation="MKVDKQLIDKLANLSKLEFSPEEAAAIEKNLSDMLDFVDKLSEV
                     DVSGVEPLVYVNPAINVMRPDEPQMDISKEDALKNAPLADSDYFKVPKVIRK"
     gene            1807..>1987
                     /locus_tag="PLL28_15180"
     CDS             1807..>1987
                     /locus_tag="PLL28_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70723.1"
                     /translation="MKIVVSGTRPTGILHLGNYYGAVRNYVRLSKDSDVRGYFFIADY
                     HSFTTHINPTELPMLV"
BASE COUNT          532 a          443 c          420 g          592 t
ORIGIN      
        1 tcaggtttta caacttgtat tgtaaacaat ctgcgtgcca aagcaaaaaa gtgctgaaat
       61 gcttaccagg aaagagttac aggattgaaa aaatatctgt atatacagga atgcaaaact
      121 atccccgtgg tcgccatgcc acctcttcaa tgccaagatc gtgtgcgatt ttgcgtgcag
      181 caacaaaaag atagtcgctc aaacggttta agtagatgag gattattggt tcaacatgtt
      241 cattttcagc cagatgcgta acaatacgtt ctgcgcggcg acaaacaact ctggccagat
      301 ggcagtatga aactgtgata tgtcctcccg gaagaataaa attttgaagt ggggccaatt
      361 cagcatccat agcgtccatg gcatcttcaa gctgcttcac atcagcatca actaaatcgg
      421 gaattgacat ttttgaacgc tccgggtcag atgccagtgc agcgccaacg gtaaataagc
      481 gatcttgaat ggtacgcaaa acattgcgaa ggtcttcatg catctgttgg tccaaaatca
      541 atcctataca aacattcagc tcatctatag taccataagc ctcgacacga aggtggtgtt
      601 tcgggacgcg agtgcctccg atcagtgagg tttgtccttc atcacccttt ttagtgtaga
      661 tcttcatgtt ttccggtaaa atttagtagt tatcaggata ccaaattttt acgtaactct
      721 gtgggattca gcttgttagc attaacttca tcacgttcga tcacgccatc gcgaattcga
      781 ataatacgat gcgcatatcg ggctatatcc tcttcatgag ttacaatgat cactgtattg
      841 ccctttgcat ggatatcttc aaacaattcc atgatctcat aactggtctt tgtatcgaga
      901 ttacctgttg gctcatcggc aagaataata gagggattat ttactaaagc tctggcaaca
      961 gcaacacgct gacgttcgcc gcccgataat tcgtttggtt tgtggtgcat gcgatgcccg
     1021 agtcctacag cctgcaatat agcagttgct ctttcaaccc tttcattgcg ttttatgccg
     1081 gcgtaaatga gcggtagagc tacattttca ataccactaa gcctggcaag cagattaaaa
     1141 gtttgaaata cgaatccaat ttcttcattt ctcaccgttg ccaactgatc ttcgttcata
     1201 cggctgacat cagtgccatt gagcacatat tggcctttgg taggtttatc cagacagcct
     1261 agaatattca tcagggtact ctttcctgag cctgaaggtc ccataatagc aacatattca
     1321 ttttttgcta tggtagtatt caagcccttc agcacctcga tcctcatttt accgaggtcg
     1381 taggttttgc ggatattgga aagcgtaatg atatcgctca taattactta cggatcactt
     1441 taggcacctt aaaataatcg ctgtcggcaa gtggggcatt tttcagcgcg tcctcctttg
     1501 agatgtccat ctgcggctca tcagggcgca taacattgat agcagggtta acgtaaacca
     1561 gaggttccac tccggataca tcaacttctg aaagcttatc aacaaaatca agcatatctg
     1621 aaaggttctt ctcgatggca gcagcttctt cagggctgaa ttccagctta gacaggtttg
     1681 ccagtttatc gatcaattgc ttatccactt tcataaggta aaggtaaggc gatttttttt
     1741 ggcaatgatg gtcttcacac agtttaacgg agggtcaatg ccatatcttt tttttatttt
     1801 cgccgcatga aaatcgtggt gagtggtacc cggccgacgg gtattttaca tttgggcaat
     1861 tattatggtg ccgtgcgcaa ctatgtgcgt ctgagcaagg attcggatgt tcggggatat
     1921 ttctttattg cggattatca ttctttcacc acacatatca atcccaccga acttcctatg
     1981 ctggtgc
//
LOCUS       DAOQHY010000331         1959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_45947_length_1959_cov_2.943803, whole genome shotgun sequence.
ACCESSION   DAOQHY010000331 DAOQHY010000000
VERSION     DAOQHY010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45947_length_1959_cov_2.943803"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PLL28_15185"
     CDS             <1..265
                     /locus_tag="PLL28_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70724.1"
                     /translation="VLKIENTNTGIGKFLYIDDIRIIPSESQAMGYVYDFITQRLVAE
                     LDDNHFTTFYEYDNEGNLIRIKKETERGIYTIQEARYNTPKSE"
     gene            269..937
                     /locus_tag="PLL28_15190"
     CDS             269..937
                     /locus_tag="PLL28_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70725.1"
                     /translation="MLKQLIFGCCTLCLWHLQAQSVDMALLAQKIKLAEAGTFSYTMT
                     ETTTASGVSDVVKTQAMIYKSEREFAMIDSSHVMIVNDSCTLYVDHASQLIIYAKNTI
                     APSGFQAQMEMTNLNSLERLPREIINDSTVIFTVENANGISKMSYNAINGDLLSISIN
                     EVARPQLYNDMPAMVTKTKQIEVNRLSSTIVNALPLVTSFISRENDSITPALNYIDYA
                     IIIN"
     gene            918..>1959
                     /locus_tag="PLL28_15195"
     CDS             918..>1959
                     /locus_tag="PLL28_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70726.1"
                     /translation="MRSLSIKSRNHLHVVPKVNMLVFRILFFAFALIFAGVKNLNGQY
                     LEQYSSEKYLKNFNPANLSTGAVSITDNPGFTGATGFTDAEVIDYVKVYFDKSLPVYF
                     SDAVKAEVVIQVKKYPIGGGSPVNENITLTVNYDPTNASLSKDIGFYTYTGYYKVGAV
                     IQSIEINDNPAISPSTLPANLKIGTGIRIARYVDFNTATAPSSVKVQYVESIDAYRIK
                     DFEVSGGLYYDIEYTYIDDYSATSLTSAIATADIAFSFKNNSTRIRVSPGDKVYIPNV
                     FDRGYLIVRARAIGPSSTDINYLVSGEWSLTDGYDDGDGMFALKTLSGVSTNYYAHIR
                     APWAMTTPTQHMA"
BASE COUNT          626 a          367 c          390 g          576 t
ORIGIN      
        1 tgttttgaaa atagaaaata caaataccgg tatcggcaaa ttcttatata tcgatgatat
       61 tcgtattata ccaagtgagt cgcaagcgat gggatatgtg tatgatttta ttacacaacg
      121 tttagtggca gaacttgatg ataatcactt tacaaccttt tatgaatacg acaatgaggg
      181 gaatttaata cgtatcaaaa aggaaaccga gcgtgggata tacacgatac aagaagcaag
      241 gtacaatact cctaaatctg aatagatcat gttaaagcaa ctcatttttg gatgctgtac
      301 gctttgctta tggcatttgc aggctcaatc agttgatatg gcgctgcttg ctcagaaaat
      361 taaactagcg gaagcaggca ccttcagcta caccatgact gagaccacaa cagcttcagg
      421 tgtatcagat gttgtaaaaa cgcaagcgat gatttataaa agcgaacgtg aatttgcaat
      481 gattgacagt agtcatgtga tgatcgtaaa tgattcatgc acgctttatg tggatcatgc
      541 aagccaattg atcatttatg ctaaaaacac catcgcacct tctgggtttc aggcgcaaat
      601 ggaaatgaca aatctgaata gtcttgagcg acttccccgc gaaataataa atgactcaac
      661 tgtcatattt acagttgaaa atgcaaatgg tatttctaag atgagctata atgccatcaa
      721 tggcgatttg ctttccatct caatcaatga agtagcacga cctcagttat ataatgacat
      781 gccggctatg gtgacgaaaa ccaagcaaat tgaagtaaac aggttgagtt caacaatagt
      841 gaatgcgctt ccattagtaa catcatttat ttcccgcgaa aacgattcta taacccctgc
      901 cttaaattat atagactatg cgatcattat caattaagtc tcgcaatcac ttgcatgtgg
      961 tgccaaaagt aaatatgttg gtattcagga ttttattttt cgcttttgca ttaatttttg
     1021 caggggtgaa aaatttaaat ggccaatatc tggaacaata cagttctgag aagtatttaa
     1081 aaaatttcaa tccggcaaat ctttccacgg gtgcggtttc aatcactgac aatccaggat
     1141 ttaccggggc caccgggttt accgatgctg aggtaataga ttatgtaaag gtgtattttg
     1201 ataagagctt gccggtctat ttttccgatg ccgtgaaagc ggaagtagtc attcaagtta
     1261 aaaagtatcc gattgggggt ggcagtcccg taaatgaaaa tatcaccttg actgttaatt
     1321 atgaccctac aaatgcatcg ttgtcaaagg atattggatt ttatacatat acaggatact
     1381 ataaagtagg tgcagtaatt caatcaatag aaataaatga taatcctgca atatcaccat
     1441 caactttacc tgcaaattta aaaatcggca caggcattcg aattgccaga tatgtggatt
     1501 ttaatacagc caccgctcct tcttctgtaa aagtacaata cgtagaatct attgatgcat
     1561 accgaataaa agatttcgaa gtgtccgggg gactctatta tgacattgaa tatacctaca
     1621 tcgatgatta ttcggctact tcgcttactt ctgctatcgc aactgctgac attgcattta
     1681 gttttaaaaa caactcaact aggatacgtg tatcgcccgg tgataaggta tatatcccaa
     1741 atgttttcga tagagggtat ctcattgtaa gagcacgtgc cattggacca tcttcgacag
     1801 atatcaacta tctggtctca ggagaatggt cacttacgga tggctatgat gatggcgatg
     1861 gtatgtttgc cttaaagaca ctttccggtg tttcaacaaa ttactacgcg catattcgag
     1921 ctccatgggc aatgactacc cctacacagc atatggcgg
//
LOCUS       DAOQHY010000332         1949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_46342_length_1949_cov_3.922386, whole genome shotgun sequence.
ACCESSION   DAOQHY010000332 DAOQHY010000000
VERSION     DAOQHY010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46342_length_1949_cov_3.922386"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="PLL28_15200"
     CDS             complement(<1..678)
                     /locus_tag="PLL28_15200"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70727.1"
                     /translation="MLREKWFLLILTACMQQAYAATPTISDSIDIRHYDINLDITDYA
                     GQTINGSTTLHIAALVDGITTLPLDLLSLDVDGITDGNGNALSFTYDEPSLIITLFTP
                     LNIGDSANIIIQYSGDPAQDGSWGGWYWSGDYSYQLGVGFDAIPHNYGRAWFPCFDNF
                     IERSTFSYDIKTTNDKKAFCGGLLIDSVDNGDGTISWHWECVQQIPSYLASVAVSKYA
                     TINDVYAG"
     gene            699..1628
                     /locus_tag="PLL28_15205"
     CDS             699..1628
                     /locus_tag="PLL28_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70728.1"
                     /translation="MICKVTKCLVLLLVVFGIQQLKAQVETSPVQIITVDSFVVHAVR
                     DGLNVQDFVQMIQEDTTFYRAFQNLRRIEYDGSATVRMFNEDHLSIASYSNRMSQFLD
                     GKCRWMQFPFEVNTGDFFDKHKQLNYYTAGVFSYIFLYRDTICGNIVETDNNAGSDDK
                     LELRKEQLKTLIFNPGRPVNGIPLIGDKMEIFSPEMQQYYDYTIAREKFATGVDCFVF
                     TIKKKPEAEKGKDVVINELTTWFDVDSKEIVARNYQLSYFTSLFDFDVSMKVRMGTID
                     GYRVPVSIKYSGYWDIPFRDPEIGNIDISVWGR"
BASE COUNT          587 a          380 c          429 g          553 t
ORIGIN      
        1 gcctgcataa acatcgttga tggtggcata tttactcaca gccacgctcg ccaggtaact
       61 cggaatttgc tgaacacatt cccaatgcca ggaaatagtg ccatcaccat tatcaaccga
      121 gtcgattaat aaaccaccgc aaaaagcctt tttatcattt gttgttttta tgtcatagct
      181 aaaagttgag cgttcaataa agttatcgaa acatgggaac caggctctgc cataattatg
      241 cggaattgca tcaaatccca cacctaattg ataactataa tcaccactcc agtaccagcc
      301 accccaagag ccatcttgtg cgggatcacc ggagtattgg atgatgatat ttgcagaatc
      361 gccaatattt agcggtgtaa ataatgtgat gatcaatgaa ggctcatcgt acgtaaatga
      421 aagtgcattg ccattgccat ctgtaatgcc atcaacatca agtgaaagca agtcgagtgg
      481 gagggtggta atgccatcaa ccagtgcagc gatatgtaag gtagtggaac catttatagt
      541 ctgacctgca taatctgtga tatcaagatt gatatcgtaa tgtcgtatat caattgagtc
      601 gctgatagtt ggtgtagcag catatgcctg ctgcatgcaa gccgtcaaaa tcaggaggaa
      661 ccatttttct cgaagcataa aatgttaatt ttacacggat gatctgtaaa gttacaaaat
      721 gcttggtgtt actcctggtg gtatttggta tacagcagtt gaaagctcag gttgaaacga
      781 gccctgttca gatcataacc gttgattcat ttgttgttca tgccgttcgg gatggcctga
      841 atgtgcagga ttttgtgcaa atgatacagg aggatactac attttatcgt gcatttcaaa
      901 accttcgacg cattgaatat gatggcagtg caaccgtacg tatgtttaac gaagaccatc
      961 tttccattgc atcttacagc aataggatgt cgcaattcct ggatggcaaa tgcaggtgga
     1021 tgcaatttcc ttttgaagtt aacaccggtg atttttttga taaacacaag caattaaatt
     1081 attacactgc cggtgtattc agttatattt ttttgtatcg ggataccatc tgtggtaaca
     1141 tcgtagaaac agacaacaat gcgggtagcg acgataaatt ggaattgcgc aaagaacaac
     1201 tcaaaacact tattttcaac cctggacgtc cggtaaacgg cattccatta ataggcgata
     1261 aaatggaaat attcagcccg gagatgcaac agtattacga ctataccatc gcgcgcgaga
     1321 aatttgcaac gggtgttgat tgttttgttt tcaccatcaa gaaaaaaccg gaagctgaaa
     1381 aaggtaaaga tgtcgtgatt aatgaactca ctacttggtt tgatgttgac agcaaggaga
     1441 ttgtagctcg aaattatcaa ttaagttatt tcacttcgct atttgatttt gatgtgagca
     1501 tgaaggtgcg tatgggcacc attgatggat atcgtgttcc tgtatccata aagtattcag
     1561 gctactggga catcccattc cgcgatcctg aaattggaaa tatcgatata tcggtttggg
     1621 ggagataggt gagtgggtca gtgggtgagt ggatgagtgt ttcgtgaatc gtgactcgtg
     1681 aatggtgagt agtgactgat cctttgactc cgctaactcc gcaaactccg cgattgaaca
     1741 agggtgtatc gtgaatcatc atttatcatc caacacattc tttaaaaccc atttaaccgc
     1801 taaggcccgg agaggaacac agagttgaga aaattcaaac tcacccactg acccacccac
     1861 gatatttgta tctgggattg gtatcaggta tcaagtatca agtatcaggt atcaagtatc
     1921 aggtaaaaaa atcacgattc acgattcac
//
LOCUS       DAOQHY010000333         1886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_49184_length_1886_cov_6.305844, whole genome shotgun sequence.
ACCESSION   DAOQHY010000333 DAOQHY010000000
VERSION     DAOQHY010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49184_length_1886_cov_6.305844"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..502
                     /locus_tag="PLL28_15210"
     CDS             104..502
                     /locus_tag="PLL28_15210"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70729.1"
                     /translation="MNFFRLYTKAAISIPLRRDRLCLFAFLIPLVFIRRILVKLISIL
                     IVEGIHSFHEIIRNKIALKIFIDADDKTLRTLRYKANIIKRGFSEDDAMKRLDLEMDE
                     YYQFLHPNKKYADKIINVDENFNYQFAEPS"
     gene            613..>1886
                     /locus_tag="PLL28_15215"
     CDS             613..>1886
                     /locus_tag="PLL28_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70730.1"
                     /translation="MKEWLLPALYLLGITYIIYKINIRITPQITAAWRIPAFLGKVAL
                     GCLNYYIWVNVIGHGDSLRYVHDSLIVYNSWFDNPKYFFELISRFSLENIPPHLAEYQ
                     KALYIEWHVPEYNMVRLLAVLNLFTGGSAWGNIILISALVYAAEVFLFKNIVRVFNAS
                     AQSAKWIFGILFFIPSVIFWSSGLLKEGPVLALLCFVGGITIGLMQKQVFAKSALNIF
                     LLLTMLIVLYGIRNYVAILCLLNLLVLRIVYHRKNIIALSLISSLLIGAIAIYCMHIR
                     TGNFFNYMQSEQGYFLLSGTDPDYTFHTLDGTAGDIIQKTPYALNNILFRPNLLHSSS
                     AFRVYQSAELMLVFAFIIWMLYTHRMHLPDRRVKLSMSPLGIVSLLLFAELLFMYGLM
                     VTDADTLCRYRSVPIMFLLLITYIGTSTSARR"
BASE COUNT          519 a          388 c          368 g          611 t
ORIGIN      
        1 cattatggaa cagaaaatgc agattaaggc ctttttctgt ccatttgagt tgtttattgt
       61 catttgtaca gctcctgttt tacgattcga agccacatag tgcatgaact tcttcaggtt
      121 gtataccaag gcagccatca gtatcccgct gaggcgggac aggctatgtt tatttgcttt
      181 tttaatacct ctagtattta ttcgccgcat attggtaaaa ttgattagca ttctaatcgt
      241 tgaaggaatt cactcctttc atgaaataat caggaataaa atagctctta aaatattcat
      301 tgatgctgat gataaaacct tgagaacgct aagatataag gcgaatataa ttaaacgtgg
      361 attctcagaa gatgacgcaa tgaaaagatt agacttggaa atggatgaat actaccaatt
      421 ccttcacccc aacaaaaaat atgctgataa aatcatcaat gttgacgaaa attttaacta
      481 ccaatttgca gaaccatcat aaagtgcggg tttctgggta acttaaacat tgtgatgccc
      541 taaaagtttg tatttattat taatgctagt tctcctacta tgccataaac aattgctcct
      601 tatctttact gaatgaagga atggctgtta ccggctttat atttattggg tattacatat
      661 attatttata agattaacat tcgtattacg cctcaaatca ccgctgcatg gcgcattcct
      721 gcatttctgg gtaaggtggc attgggttgt ctcaattatt atatatgggt gaatgtgatc
      781 ggtcatggtg atagtctgcg ctatgtacac gacagtctca ttgtatataa cagctggttc
      841 gacaatccga aatacttttt tgaattgata agccgcttct ccctggaaaa tatcccgccg
      901 catcttgctg aatatcaaaa agccctatac atcgaatggc atgtgcctga atacaatatg
      961 gtgcgactgc ttgcagtatt aaacctcttt acaggcggca gtgcttgggg aaatatcata
     1021 ttgatatcgg cactggtgta tgccgctgaa gtttttctgt ttaaaaatat tgtgcgagta
     1081 tttaatgcat ctgcccaatc ggcgaaatgg atattcggta ttctcttctt tattccatct
     1141 gtcatattct ggagcagcgg actattaaaa gagggacccg ttttggcatt gctttgtttt
     1201 gttggcggaa ttaccatcgg cctgatgcaa aagcaagtgt ttgctaaatc ggcactaaac
     1261 atatttctcc ttctcacaat gctcattgta ttgtatggca taagaaatta cgtggccata
     1321 ttatgtctgc tcaatttatt ggtgctgcgc atagtatatc acagaaaaaa catcatcgcc
     1381 ctcagtctta tcagctccct gttaattggt gccatcgcta tttattgcat gcatatccgc
     1441 acaggaaatt tctttaatta catgcaatcg gaacagggct atttcttact ctccggcacc
     1501 gatcccgatt acacatttca tacgctggat ggaacggcag gtgatatcat ccaaaaaact
     1561 ccctacgccc tgaacaacat tttgttccga ccaaatctgt tgcatagtag ttccgctttc
     1621 cgtgtttacc agtcggcgga attaatgctc gtatttgcat tcattatttg gatgctctac
     1681 actcaccgaa tgcacctgcc tgatcggaga gtcaagctca gtatgtcgcc attgggaatt
     1741 gtttccctgc tattgttcgc cgagctcttg ttcatgtacg gactgatggt aaccgatgcc
     1801 gatacgcttt gtcgctatag gtcagttcct ataatgtttc ttcttctgat cacatacatc
     1861 ggaacaagta catctgcgcg ccggaa
//
LOCUS       DAOQHY010000334         1870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_49961_length_1870_cov_6.004959, whole genome shotgun sequence.
ACCESSION   DAOQHY010000334 DAOQHY010000000
VERSION     DAOQHY010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49961_length_1870_cov_6.004959"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="PLL28_15220"
     CDS             <1..828
                     /locus_tag="PLL28_15220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNF70731.1"
                     /translation="NILANKSTKALTEERLQMDADVIGQVQDEVNSWAEEKRSEIAIL
                     HEQANFRKEFLGNVSHELKTPIMSIQGYLENLQDGGIEDPGVNYLYVEKALKNVERMA
                     AMIDDLVDISKLESGELNIKKSKFDICSLTKDVFEALEIQSEQSRISLSIKEGCDHIY
                     LVNADQKLIYEVLINLISNAIKYGKKEGFVHVGFYRMGDRILTEISDNGVGIAQEHLT
                     RVFERFYRIEKSRSRDNGGTGLGLSIVKHIIEAHDQIVNVRSTEGEGSTFSFTLDVA"
     gene            complement(831..1238)
                     /gene="msrB"
                     /locus_tag="PLL28_15225"
     CDS             complement(831..1238)
                     /gene="msrB"
                     /locus_tag="PLL28_15225"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNF70732.1"
                     /translation="MSKKQYAIIKSDQEWQDMLTPEQYQILRKKGTERPFTGEYDHFF
                     GSGVYRCAGCGQLLFDSTSKFDSGCGWPAFDKEIADGNIDYESDNSFGMRRVEIMCSN
                     CGGHLGHVFDDGPTETGQRFCVNSASLKFDEKK"
     gene            complement(1432..>1870)
                     /locus_tag="PLL28_15230"
     CDS             complement(1432..>1870)
                     /locus_tag="PLL28_15230"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNF70733.1"
                     /translation="TLIGKTGQPLILQDINGHDIPLYAVDRRFTVILFWSPTCGHCKK
                     ELPKVEKIYPELKELGAEIYAVYNEEEFDKWEDWLHQHNYPWINVCDRNNKEMVGVKY
                     NVDMTPLIYVLDHNKKIIGKKISIEQVPDLLKNQIAIENAHKP"
BASE COUNT          572 a          380 c          367 g          551 t
ORIGIN      
        1 aacatacttg caaataaatc taccaaagca cttacggaag aacggcttca aatggatgct
       61 gatgtaattg gtcaggtgca ggatgaggta aattcatggg cagaggaaaa gcgttcagaa
      121 attgccattc tgcatgaaca agcaaatttc cgcaaagaat ttttagggaa cgtaagccat
      181 gagttaaaga caccgataat gagtattcaa ggataccttg aaaaccttca ggatggcggc
      241 attgaagatc cgggcgttaa ttatttatat gttgaaaagg ccttgaagaa tgttgaacgc
      301 atggctgcca tgatagacga cctcgtagat atcagcaaat tggaatcagg tgaattaaac
      361 attaaaaaat caaagttcga tatctgttcc ttgaccaaag atgttttcga agcgttggaa
      421 atacagtctg aacagtcgcg catttcatta tccattaaag aaggttgtga tcatatatac
      481 ctggtgaatg cagatcaaaa gctcatttac gaagtgttaa tcaatttaat aagcaacgca
      541 attaaatacg gtaaaaaaga ggggtttgtg catgttggtt tttaccgaat gggcgataga
      601 atccttactg aaatttcaga caatggcgtg ggtatagcgc aagaacattt gacacgcgta
      661 tttgagcgtt tctatcgcat tgagaaaagt cgaagcagag ataatggcgg aaccggtctc
      721 ggtctctcaa tcgttaaaca cattattgaa gcacatgatc agatagtaaa tgtgcgcagt
      781 acagaagggg agggcagtac attcagcttc accctagatg ttgcctagga ttatttcttc
      841 tcatcaaact tcagcgaagc cgaattcacg caaaaacgct gaccagtttc tgtaggccca
      901 tcgtcaaaca catgtccaag gtgaccgccg caattactac acattatttc tacgcgtcgc
      961 atgccaaaag aattatcaga ttcgtaatcg atattgccgt cggctatttc tttatcgaat
     1021 gccggccaac cgcatccgct gtcaaacttt gatgtagaat caaataacaa ctgaccacaa
     1081 ccggcacagc ggtaaactcc tgacccgaaa aaatggtcgt attcacctgt aaacgggcgt
     1141 tccgtcccct ttttgcgaag gatctgatat tgctcaggag taagcatatc ctgccattcc
     1201 tgatctgatt ttatgatggc gtattgcttt ttcgacatac aattattaca tacgtattca
     1261 acctgctaaa agttcatgaa caattctcaa caattacttg tttaacagtc aaacaaccgc
     1321 tatgaaaccg ggatactcga aatatgtgtt tgccatcata ctatttttcg gaatcatgtc
     1381 aatcatgata ggtatggcat tattgacgca ccacgaaatt tcgatttttg attaaggttt
     1441 atgggcattt tcaatagcaa tctgattttt cagtaaatca ggcacctgct caatcgatat
     1501 tttcttacca atgatttttt tgttgtgatc taatacatag atcaatggcg tcatatctac
     1561 attatatttt acgcccacca tttctttatt gtttctgtcg catacattga tccacggata
     1621 attatgctga tgaagccaat cttcccattt gtcaaattct tcctcattat aaacagcata
     1681 tatttcggca cccagctctt ttaattcagg gtaaattttt tccacttttg gcaattcctt
     1741 tttacagtga ccacacgttg ggctccaaaa taaaatcacg gtaaatcgcc tgtcaacagc
     1801 atacagaggt atatcgtgcc catttatgtc ttgtaagatc agcggctgcc cggttttacc
     1861 tatcaaggtg
//
LOCUS       DAOQHY010000335         1863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_50288_length_1863_cov_4.943584, whole genome shotgun sequence.
ACCESSION   DAOQHY010000335 DAOQHY010000000
VERSION     DAOQHY010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50288_length_1863_cov_4.943584"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /gene="era"
                     /locus_tag="PLL28_15235"
     CDS             complement(<1..503)
                     /gene="era"
                     /locus_tag="PLL28_15235"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNF70734.1"
                     /translation="MSYRSGFVNIIGRPNVGKSTLLNVLIGEKLSIITPKAQTTRRRI
                     LGIVTTDQYQAVFSDTPGLIDQPEYKLHQWMNAQVSTALKDGDVLVFMTDPKDELNAD
                     HPLIQTLQQIRTPVIVVINKSDIIAKNVLEQLIIKWTSVLPKATIISISALQKINTDK
                     LFQHIVDA"
     gene            613..685
                     /locus_tag="PLL28_15240"
     tRNA            613..685
                     /locus_tag="PLL28_15240"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:646..648,aa:Met,seq:cat)
     gene            818..>1863
                     /gene="der"
                     /locus_tag="PLL28_15245"
     CDS             818..>1863
                     /gene="der"
                     /locus_tag="PLL28_15245"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNF70735.1"
                     /translation="MGFTVAIIGRPNVGKSTLFNRLTGDRHAIVDDLSGVTRDRHYGM
                     VDWNGKIFDLIDTGGFVAHSSDVFEREIRRQVQIAIEEASLLLFMMDVTTGITDFDDD
                     VIKLLRRCGKPVFVIVNKVDNNRRHLDATEFYAAGFPDIFFLSSISGSGTGELLDAVA
                     ARIPGEDASELKELEALPRFAIVGQPNVGKSTLLNALTGQERTLVTDIAGTTRDAIHT
                     RYRLFDKDFVLVDTAGVRKKSVVKEDLEFYTVIRTIKAIDECDVCMLMIDATTGIEAQ
                     DLAILNMIEKKKKGVVILVNKWDLVAKETNTMLRMEEVIRERVAPFRDIPIIFISAVD
                     KTRIFKAMETGLEV"
BASE COUNT          516 a          379 c          447 g          521 t
ORIGIN      
        1 gcatctacta tatgttgaaa taatttgtcg gtattaattt tttgtaatgc tgaaatggaa
       61 ataatggttg cttttggcag cacggaagtc catttaataa tcagttgctc caacacattt
      121 tttgcaataa tgtcgctttt attaatgact acaataacag gtgtacgaat ttgttgcagg
      181 gtttgaatta gcggatgatc ggcatttagt tcatctttag gatcagtcat gaataccaat
      241 acgtcgccat cttttaaggc ggtacttacc tgcgcattca tccattgatg cagtttatat
      301 tccggttgat caattaagcc cggtgtatct gaaaataccg cctgatattg atctgtagta
      361 acaataccca gtattcttct tcgtgtagtt tgtgcttttg gtgtgatgat agacagcttt
      421 tcaccaatca atacattcaa caaggtcgat tttccaacat ttggtctccc aatgatattc
      481 acgaagccgg agcgataact catggcgcaa aattacggtt ttggagagcc aatcgctgat
      541 aatattaggt agaatggcaa aaacaacgta tctttgcacc tcgttctgaa agaaatttgc
      601 ttcaagatat atcgcggggt ggagcagttg gtagctcgtc gggctcataa cccgaaggcc
      661 acaggttcga gtcctgtccc cgctacacag aaacccggta tctccgggtt tttttatatc
      721 actaactgaa gcaactgatt tcaaacctgt caatgcaatg agggcgctgc actgatagcc
      781 ttaaatcgcc cgcaatacaa aataatcacc cgatctcatg ggatttaccg ttgccatcat
      841 aggaagacca aacgtaggga aatcgacgct gtttaacagg ttgacgggcg acaggcatgc
      901 cattgtggat gaccttagtg gggttacccg cgacaggcat tatggcatgg ttgactggaa
      961 cgggaagatt ttcgacctga tagatacagg aggttttgtt gcacatagca gtgatgtgtt
     1021 tgaacgtgag atccgccgtc aggtgcagat tgccatagaa gaggcatcgc ttttgttgtt
     1081 tatgatggat gtgacaaccg gcatcaccga ctttgatgat gatgtgatca aattattgcg
     1141 tcgctgcggg aagcctgtct tcgtgatcgt gaataaggta gataataatc gccgacatct
     1201 cgatgccact gagttctatg ctgccggctt tccggacata tttttccttt catctatctc
     1261 aggaagtggc accggagagt tgctcgatgc cgtagcagcg cgcattccgg gtgaagatgc
     1321 cagtgagctc aaagaactgg aagcactgcc acgcttcgct attgtaggtc agccaaatgt
     1381 cgggaaatcg acactattaa atgcacttac cgggcaggaa cgcacattgg tgaccgatat
     1441 tgccggaacc accagagatg ccatccacac ccgatataga ctgtttgata aggattttgt
     1501 gttggtagat actgcaggtg tgcgcaaaaa atctgtggta aaagaggacc tggagttcta
     1561 tactgttatc agaacgatta aggcgattga tgaatgtgat gtgtgcatgt tgatgattga
     1621 tgccactacc ggaattgaag cacaagacct tgccatcctc aatatgattg agaaaaagaa
     1681 aaaaggtgtg gtcattttgg tcaataaatg ggaccttgtt gccaaggaaa ccaataccat
     1741 gctgagaatg gaagaggtca tccgcgagcg ggtagcgcca ttccgcgata tccctatcat
     1801 ttttatatcg gcagttgaca aaacacgaat tttcaaagcc atggaaacag ggctggaagt
     1861 ata
//
LOCUS       DAOQHY010000336         1844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_51247_length_1844_cov_4.581330, whole genome shotgun sequence.
ACCESSION   DAOQHY010000336 DAOQHY010000000
VERSION     DAOQHY010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51247_length_1844_cov_4.581330"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PLL28_15250"
     CDS             <1..542
                     /locus_tag="PLL28_15250"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNF70736.1"
                     /translation="YSATFKRYPLIGNMMVALLAGISILMTFIYEPNLYQLARPGDYF
                     IAGICTKFIIALGVIAFTSTMVREIVKDLEDMEGDDRYGAKTMPIAWGINIAKIIAVV
                     FVLLTLLGIGYIFTNIIAAEGIIYGAYIGVMLGFWVAVSVRLFLSRNAKQYHTLSTMI
                     KIGMLIGLLIMPLYSIVEF"
     gene            544..1107
                     /locus_tag="PLL28_15255"
     CDS             544..1107
                     /locus_tag="PLL28_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HNF70737.1"
                     /translation="MKIILASGSPRRSHILSTAGFNFTVMVPDVEETFPDSMPVMDVP
                     LFIADKKLQAVLTDTSVDNIVITADTVVILQDRIIGKPIDEADAFQILKSLSGNMHLV
                     VSGVCIAYQGKQYDIEVTTKVYFDHLTDEEISKYLQECKPFDKAGAYAIQEWIGMNKI
                     TRIEGDYYNVVGFPMSKIYPVLTALME"
     gene            complement(1108..>1844)
                     /locus_tag="PLL28_15260"
     CDS             complement(1108..>1844)
                     /locus_tag="PLL28_15260"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNF70738.1"
                     /translation="YQPRAISITHIPNNTGMIQPVEQIGDLCEKYNLWYFVDACQSAG
                     QIPLDVTSIKCDFLSFSMRKFLRGPRGCGMLYVSDKALKAGLEPLFIDMRGADWTEAN
                     KYQPVPDAKRFEYVEQSYGIITGAIAAMRYYLNVGPDQIALYNAELCAYARQQLSAIP
                     HIHLQDRGARLSAIISLTSDRSDPMYFKEILNRNGINAGAASRKFALLDFDQKGVSGA
                     VRISPHYYNTFEEVDKMIAVIKENAI"
BASE COUNT          529 a          366 c          389 g          560 t
ORIGIN      
        1 tttattctgc aacctttaag cgatatcctt taatcggaaa tatgatggtt gctttattgg
       61 ccggcataag cattttgatg acatttattt atgaacccaa cctgtatcaa cttgcaagac
      121 ccggagatta tttcatcgcc ggaatttgca ctaaatttat aatcgctctt ggtgtgatcg
      181 ctttcacatc aaccatggtt agggaaattg tgaaagacct tgaagatatg gaaggcgatg
      241 atcggtacgg cgctaaaaca atgcccattg cctggggaat aaatatcgct aagattatag
      301 ctgttgtttt tgtattattg acgctgctgg gtatcggtta tatatttaca aacattattg
      361 cagcggaagg tatcatatat ggcgcctata ttggcgtgat gcttggcttc tgggttgcag
      421 tgagtgttcg tcttttcttg tcaagaaacg ctaagcaata tcacacacta agcaccatga
      481 taaaaatagg tatgcttatc ggtttattga tcatgccttt gtattcgatc gtagaatttt
      541 aatatgaaga tcattcttgc atcaggctct ccgcgccgca gtcatattct ctcaactgcg
      601 ggatttaatt ttacagtcat ggtgccggat gtggaagaaa cgtttcctga ttccatgccg
      661 gtaatggatg tgcccctgtt catagcggac aaaaaattgc aggcagtgct tacggataca
      721 tccgttgaca atattgttat caccgcagat acagttgtaa tattacagga caggattatc
      781 ggtaaaccga ttgatgaagc tgatgctttt cagattctaa aatcgttaag cgggaatatg
      841 catcttgttg tttccggtgt ttgcatcgca tatcaaggaa agcaatacga tattgaagtg
      901 actaccaaag tgtattttga tcatcttacc gatgaagaga tttcgaaata cctccaagaa
      961 tgtaagccat ttgataaggc aggtgcatac gccattcaag aatggatagg catgaataag
     1021 attacgcgca ttgaaggcga ctattacaat gtagtaggat ttcccatgag caagatttat
     1081 ccggtgttga ctgcattaat ggaatgatca gattgcattt tccttgatca ctgcaatcat
     1141 cttgtccact tcttcaaagg tattataata gtgtggtgaa atgcgaacgg cgccacttac
     1201 tcctttttga tcgaaatcca ataacgcaaa tttccgtgag gctgcgcccg catttattcc
     1261 gtttctgttc aatatttctt tgaaatacat cgggtcactg cgatccgagg tgagcgaaat
     1321 aatggcactc aaacgcgcac ctctatcctg caaatgaata tgcggaatgg ctgaaagctg
     1381 ttgtcttgca taggcacaca actccgcgtt atataaagca atctgatcag gtccaacatt
     1441 taaataatac cgcatggctg ctatggctcc cgtaatgatg ccatacgatt gttccacata
     1501 ctcaaaacgc ttggcatcgg gaactggctg atatttattt gcttcagtcc agtcggcacc
     1561 acgcatatca ataaataatg gttctaaacc ggccttaagt gctttatcag aaacatataa
     1621 cattccgcaa ccacgtggac cacgcagaaa ttttcgcatg gagaatgaaa gaaaatcaca
     1681 tttaatggat gtaacatcta atggaatttg tcctgcactt tggcaagcat ccacgaaata
     1741 ccagaggtta tatttttcgc acaaatcacc aatttgttca accggctgaa tcatgccggt
     1801 attgtttgga atatgtgtta ttgaaatcgc acggggctga tatt
//
LOCUS       DAOQHY010000337         1841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_51380_length_1841_cov_4.307391, whole genome shotgun sequence.
ACCESSION   DAOQHY010000337 DAOQHY010000000
VERSION     DAOQHY010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51380_length_1841_cov_4.307391"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1821)
                     /locus_tag="PLL28_15265"
     CDS             complement(<1..1821)
                     /locus_tag="PLL28_15265"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNF70739.1"
                     /translation="MKKILLMSAMLCTIAAFAQQTETTYDLNEVIVSGNRFPEERREV
                     AQKVDVIKTSTINFTNAATTAELLSESGKVFVQKSQLGGGSPVLRGFEASRVLMVVDG
                     VRMNNAIYRAGHLQNVITLDQSILDRVEIAYGPSSLVYGSDALGGVMHFHTKNPKLLV
                     DGESMASGSAFLRYATAADAMSGNFNINLAGKKIGSLTSVSYNDYNDLRSGTVENDEN
                     KNYWTRPYYVVTTNGVDELKANDDKYTQVYSGYTQYDVMQKFLYQQSAKLSHKLNFQY
                     STSTDIPRYDRLTDPLNATTGDSLRFAEWYYGPQDRMLVAYDMLYAGKTAAFDNMHVI
                     LSYQDIQESRHQRRWNKTNLQHRIEDVSVMGANIDFEKKINANTITYGLESYFNDVTS
                     SAHEEDILTGEETPLDTRYASGGATMMNAAAFASHQLKFSNNKFVLNDGLRFNYSKLT
                     ASFTDKTFFPFPYTDIDQQNTSLTGSLGLVYSPDNSTKISLIGSTGFRVPNVDDLTKV
                     FESGPGSLIVPNPDLGPEKTINADLSIVKVFKDKLEVELTGFYTRFNDYIALEPGTFE
                     GADSIEYDGVLSEVLTTVHKGSAYLYGFNVGADVNIINGLN"
BASE COUNT          526 a          381 c          366 g          568 t
ORIGIN      
        1 attcagtcca ttgatgatat ttacatcggc accaacatta aatccataga ggtatgcact
       61 tcccttgtgt acagttgtga gcacctcgct caacacacca tcgtattcaa ttgaatcagc
      121 accttcgaat gttccgggtt ccaatgctat ataatcatta aatcgcgtat aaaatccggt
      181 cagttcaact tcaagtttgt ctttgaacac tttcacaatt gataagtcgg cattgatagt
      241 tttttcaggg ccgaggtcag gatttggaac gataagactt cctggaccgc tttcaaaaac
      301 tttggtaagg tcatcaacgt ttggcacgcg gaaaccggta gaaccgatca aggatatctt
      361 tgttgaatta tcaggcgaat acactaaacc gaggctacct gttaacgaag tattttgttg
      421 atcgatatct gtataaggga atgggaagaa tgttttatca gtaaacgatg ctgtcaattt
      481 tgaataatta aaacgcaggc catcatttaa tacaaattta ttgttgctga atttcaattg
      541 gtgcgaagca aatgcagcag cattcatcat ggtagcgcca ccgctggcat aacgagtgtc
      601 taaaggtgtt tcttcacctg taagaatatc ttcttcatgt gcagacgatg ttacatcgtt
      661 gaaataagat tctagtccgt aagtgatggt gtttgcattg atttttttct cgaaatcaat
      721 gtttgccccc attacactaa catcttcgat acgatgttgc agatttgttt tattccagcg
      781 acgttgatgg cgactttcct gaatatcctg atagctgaga atcacatgca tattgtcgaa
      841 agcagctgtt tttccggcat acaacatatc ataggcaaca agcatcctat cttgtggacc
      901 ataataccac tcagcgaagc gaagactatc gcctgtagtt gcattcaatg gatcagttaa
      961 acgatcatag cgcgggatat cggtagatgt tgagtattgg aaattcaatt tgtgtgaaag
     1021 ctttgcactt tgctgataca ggaacttctg catgacatca tattgcgtat atcctgaata
     1081 cacctgtgtg tatttatcat catttgcttt caactcatca acgccgtttg tcgtaacaac
     1141 atagtatgga cgcgtccaat agtttttgtt ttcatcattt tcaactgttc ccgaacgcaa
     1201 gtcattataa tcattatatg atacagaagt aagcgaaccg atttttttcc ctgcgagatt
     1261 aatattgaaa ttacctgaca tggcatctgc ggcagttgcg taacgcagga aagcagatcc
     1321 tgaagccatg ctttcaccat caacaagcag ctttggattt tttgtatgga aatgcataac
     1381 accacccagt gcatcgctgc catatacaag agatgaaggg ccataagcaa tctccactct
     1441 atcaagaatt gactgatcga gtgtaatcac attttgaagg tggccggcgc gatagatagc
     1501 attgttcatg cgcacaccat ccacaaccat caatacacgg ctggcttcga acccacgcaa
     1561 taccgggctg ccaccaccga gttgactttt ctgcacaaat acttttcctg attctgaaag
     1621 tagttcagca gtggttgcag catttgtgaa attaattgtc gatgttttta taacgtcgac
     1681 tttctgagct acctcacgac gttcttccgg gaatcgattt cctgaaacga taacttcgtt
     1741 aagatcgtat gtagtttctg tttgctgagc gaatgcagct atagtacaga gcattgcgct
     1801 cattaataaa atttttttca taatatgtaa ttgagtgaaa t
//
LOCUS       DAOQHY010000338         1820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_52488_length_1820_cov_3.311615, whole genome shotgun sequence.
ACCESSION   DAOQHY010000338 DAOQHY010000000
VERSION     DAOQHY010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52488_length_1820_cov_3.311615"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="PLL28_15270"
     CDS             complement(<1..1042)
                     /locus_tag="PLL28_15270"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNF70740.1"
                     /translation="MLTALATPAKAQKFPDLHFENIYTTDGLSDNSVTCLMEDSQGFL
                     WIGTSNGLNRYDGNTFDVFFHEEGNTNSLSGNIISGIIEDNTGVIWIGTRDGGITRYD
                     PQAKLSQRFTRFTHVPDDPKSIYSNRIITLAELNDTYIIFSTEGISSGFIDRKTYQIT
                     YHNISDTLSAITDPRTALPTPDGNSWVQRFQRVGSQIYLSRLIGGYAEVYDIEHPKRR
                     KLKSSGSAGSVQCFAIDDDRIWVGGWVPGLYVHPHPSSVPDGSILPVSKIVDVPSEVM
                     SIISWDAQYMLAASKGSGLYIVDKSTHAAKAFTYDRADPYSIAGNKIYCLLKDSRGIL
                     WVGTSNGLSKYHA"
     gene            1221..>1820
                     /locus_tag="PLL28_15275"
     CDS             1221..>1820
                     /locus_tag="PLL28_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70741.1"
                     /translation="MKTLCLGIACLFCLHLNAQTIYAVDQSDGFKFGIIHPEDGSFTT
                     LHAFTENIYRINSGSASDPNAGYYFVEVKDENEDHFLYTIDITTGDVLYDPALSEPLF
                     ELNYSCHFDLLFGFEYTDTATNMVSINHMTGETTLLASYPEIDFLYMMTGSYNEKYSE
                     FLCWVKLVDDDNYYKTMFDIPTGEILLQVEESWQFDNMYN"
BASE COUNT          513 a          401 c          399 g          507 t
ORIGIN      
        1 ctgcatgata tttgcttaat ccatttgatg tacctaccca aagtatgccg cgactatcct
       61 taagcaaaca atagatctta tttccggcaa tgctataggg atccgctctg tcgtaagtga
      121 aagcttttgc agcatgtgta gatttatcta cgatatacaa tccactgcct ttggaagcag
      181 ccagcatata ttgtgcatcc caggaaatga tggacatcac ttcagagggc acatcaacta
      241 ttttgcttac gggtaagatg ctcccgtcgg gtactgaaga tggatgtgga tgtacataca
      301 atcccggcac ccatccacct acccagatgc ggtcatcgtc gatggcgaaa cactgcacgc
      361 tgcctgccga tccgcttgat tttagttttc tgcgtttcgg atgttcaata tcatacactt
      421 ctgcatatcc accgatcaat cgggaaagat agatctgcga acctacgcgc tgaaatcttt
      481 gcacccagct gtttccatcc ggtgtcggta aggccgtgcg cgggtcagtg atcgcgctga
      541 gggtatccga tatgttgtga taggtgatct gataggtttt tcgatcaatg aatcccgatg
      601 agatgccttc ggtgctgaaa ataatatagg tatcattcaa ttcggccagg gtgataatgc
      661 gattggagta aatacttttc gggtcatcag gaacatgggt aaaccgggta aatcgttggg
      721 agagtttagc ctgcggatca tagcgggtaa taccaccatc gcgggtgccg atccagatca
      781 ctccggtatt atcttctatg ataccggaaa taatattgcc cgagagggaa ttggtatttc
      841 cttcctcgtg aaaaaataca tcaaaggtat ttccatcata ccgatttaat ccatttgagg
      901 tgccgatcca taaaaaaccc tgactatcct ccatcaggca ggtgacagaa ttgtcgctta
      961 atccatctgt agtgtagatg ttttcaaaat gcaggtcagg aaacttttgc gcctttgccg
     1021 gagtagcaag agcagttagc aaaaggcata ataaacatat cctgtacatg gcgaaataca
     1081 tgtcaggcta agatacaaat taggcagaaa acccaatttc acgacaaggg atatacatgg
     1141 tatatgcaat acacccaaaa aaacacgccc aataatgtgc aacagttatg tatatttgta
     1201 tagataaata ctgattacct atgaaaacac tctgcctggg cattgcctgc ctgttttgcc
     1261 tgcacctgaa tgcacaaacg atttatgctg ttgaccaatc tgatgggttc aagtttggca
     1321 tcatacaccc tgaagatggt agctttacta ccctccatgc ttttacggaa aatatctatc
     1381 ggatcaactc aggaagtgcc agcgacccga atgccggtta ttactttgtt gaggtgaagg
     1441 acgaaaacga ggaccacttt ttgtatacga tagacatcac aaccggcgat gtgttgtatg
     1501 atcccgcatt atccgagcct ttgtttgaac tcaactacag ttgtcatttc gacctgctct
     1561 ttggctttga atatactgac accgcaacga atatggtcag catcaatcac atgaccggtg
     1621 aaaccaccct gctcgcttcc tatccggaga tcgatttcct gtacatgatg accggatcct
     1681 ataatgaaaa gtatagcgaa tttttgtgct gggtcaaatt ggtggatgat gataattact
     1741 ataaaaccat gttcgacata ccgaccggtg aaatattgct tcaggtggaa gaatcctggc
     1801 agtttgataa tatgtataat
//
LOCUS       DAOQHY010000339         1747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_56464_length_1747_cov_6.351064, whole genome shotgun sequence.
ACCESSION   DAOQHY010000339 DAOQHY010000000
VERSION     DAOQHY010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56464_length_1747_cov_6.351064"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PLL28_15280"
     CDS             complement(<1..371)
                     /locus_tag="PLL28_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70742.1"
                     /translation="MKIIKTVFLSALTIGASQQLHAQDVHLHVNTQWEECSIQLDSTL
                     SQGAWHQFTKEAGMVVYFRPLHGAETLAPGKFELSVLQWNTGIDETQSAWNETFVHPD
                     TTHYLIGGDELPFPGLTLRAG"
     gene            complement(505..1653)
                     /locus_tag="PLL28_15285"
     CDS             complement(505..1653)
                     /locus_tag="PLL28_15285"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNF70743.1"
                     /translation="MSLLLLTKKNKGAADRILLLWMVFITLHQLYYYFDHSAYFRTYP
                     AFVGFDFPLPLLHGPLLFLYAAALVGRLPEKWWQRLAHFIPALACLMYMTKFFMLTGS
                     EKYYIIDNEGVGYELFLLIRFIAIILSGIVYVVLTLILLNRHQRSIRENFSDIEHINL
                     KWLQYLTAGISLIWISVIFGTDEITFSIVVIFVLFIGIYGIRQTPIFASGHPLGTPIS
                     IVGEQVNNAIDHADTNIDAGDVVTNEPQDIIAQTKYRKSGLSEGDIDALHRKLTDLML
                     KEALYTQPDITLGEVARRLDTHPNYLSQVINARLQKTFYDYINAHRVEAFTQLVSDPA
                     QRRYTLLALAFQCGFNSKSSFNRNFKKVMGESPSDYLDRMQVVLDGEV"
BASE COUNT          534 a          371 c          377 g          465 t
ORIGIN      
        1 atgcctgcgc gaagggtaag tccgggaaat ggcaattcat caccaccgat caggtaatgt
       61 gtagtatccg gatgaacgaa ggtctcgttc caggcagatt gggtttcgtc gatacctgtg
      121 ttccattgta aaaccgagag ttcgaattta cccggagcta gggtttctgc tccgtgaagc
      181 ggcctgaagt aaacaaccat acccgcctcc ttagtgaact gatgccacgc accttgtgaa
      241 agcgtggaat ccagttgtat ggaacattct tcccattgtg tgttaacatg caaatgcacg
      301 tcctgagcat gcaattgttg tgaggcgcct atagttaatg cggataaaaa aactgttttg
      361 atgattttca ttttatacga tttatgcggc aaaggaacgg tgatcataag ctcctgtcgc
      421 ttcatcttat aagatgacac caataaatac aggcttttga gcccgaatgc gtaaattttt
      481 agaaatatta acgatagccg gggatcagac ttctccatcc aaaaccacct gcatgcggtc
      541 gaggtaatcg gatggcgact cacccatcac ttttttaaag ttgcggttga acgatgattt
      601 cgaattgaaa ccacattgaa atgcaagggc caatagcgta taccggcgtt gagcaggatc
      661 actcaccaat tgtgtgaaag cctccacccg atgtgcattg atataatcgt aaaaagtttt
      721 ttgcaagcgc gcattaatca cctgcgagag gtaattcgga tgagtatcaa gtcttcgggc
      781 tacctcgcca agggtgatat caggttgtgt atataacgcc tctttcagca tcaaatcggt
      841 caactttcga tgtagtgcat cgatgtcccc ctcgctcaat ccggattttc gatattttgt
      901 ttgggcgatg atatcctggg gttcatttgt aacaacatcg ccagcatcaa tattggtatc
      961 ggcatggtca attgcgttgt ttacttgttc tccgacgatg gaaattggag tgccaagggg
     1021 gtgtccgctt gcaaaaatcg gtgtctgtct gatgccgtaa atgccaatga ataaaacaaa
     1081 aataaccact atcgaaaacg ttatttcatc agtcccaaaa attaccgata tccatatcaa
     1141 gctgataccg gcagtgagat attgaagcca ttttaaatta atatgttcga tatccgaaaa
     1201 attctcgcgg atgctgcgct gatgtcgatt taataatatc aaagtgagca caacataaac
     1261 tattcctgat aaaatgatgg caataaatct gattagcaga aataattcgt atcctactcc
     1321 ttcattgtcg atgatataat atttttcaga accggtcaac ataaagaatt ttgtcatgta
     1381 catcaaacat gccagcgcgg gaataaaatg cgctaaacgt tgccaccatt tttccggcaa
     1441 tcgcccaacg agtgcggcag cataaagaaa taacagcgga ccatgtaaca agggcaaagg
     1501 aaaatcaaag ccaacaaacg cgggataagt tctaaaataa gcagaatgat caaaataata
     1561 atacaattga tgcagggtga tgaagaccat ccacagcagc aatatcctgt cggccgcacc
     1621 cttgtttttc ttagtgagca acagcagtga caaaaacaat gagattacaa tgcctgcaat
     1681 tattatcatc ccccgcaaat ctagctaaac attgcaggca aatgcaaact caatggtgag
     1741 aggtgag
//
LOCUS       DAOQHY010000340         1698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_59466_length_1698_cov_3.355447, whole genome shotgun sequence.
ACCESSION   DAOQHY010000340 DAOQHY010000000
VERSION     DAOQHY010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59466_length_1698_cov_3.355447"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="PLL28_15290"
     CDS             complement(<1..927)
                     /locus_tag="PLL28_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNF70744.1"
                     /translation="MADEIQSIKQRFGIIGNAPGLNYALSIAARVAPTNLTVLITGES
                     GVGKEIFSQIIHQYSTRKHGPFIAVNCGAIPEGTIDSELFGHVKGSFTNAVDDRKGYF
                     ETVNGGTIFLDEVGELPHGTQARLLRVLEAGEFIRVGSSKVQKTDVRVVAATNVDLHK
                     GIAEGKFREDLFYRLNTVPISVPALRERKDDIHLLFRKFTSDFSEKYRTLAITLDSDA
                     VELLRNYNWPGNIRELKNIAEQVSVLATDKMVTAAELHQFIPQQRNSMLPMISGKQTG
                     NDFTERDLLYKVLFDMKQDLNDLKQFVLHMAEA"
     gene            complement(933..>1698)
                     /locus_tag="PLL28_15295"
     CDS             complement(933..>1698)
                     /locus_tag="PLL28_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNF70745.1"
                     /translation="TLLGQNVDSYKWENPETGKVVNFAELMAMVASVDSTLRVRFSTS
                     HPKDITDEVLHTMARFDNICNYIHLPVQSGNSRVLELMNRTYDREWYLDRINAIRNIV
                     PDCGISTDVITGFCSETEEEHQDTLSLMEMVQYDLAYMFFYSERPGTLAARKYTDDIP
                     HETKIRRLNEIIALQTKHSHARNQADIGKTFTVLVEKPSKRSALDMCGRNDQNKMVVF
                     PATDVNPGDYVQVRVTSATSATLIGEMVGRGMHKAV"
BASE COUNT          440 a          416 c          351 g          491 t
ORIGIN      
        1 tgcctctgcc atatgaagca caaactgctt gagatcattg agatcttgtt tcatatcaaa
       61 caacacctta tacagcagat cgcgctcagt aaaatcgttt cccgtttgct ttccggagat
      121 cataggaagc atactattgc gttgttgcgg gataaactga tgcaattcag cagctgtaac
      181 cattttgtct gttgccagaa cggaaacctg ttcggcaata ttttttaatt cacgaatatt
      241 tcccggccag ttataattgc gcaaaagctc tacagcatca gaatcaagcg tgatagctaa
      301 tgttctgtat ttctcgctga aatcgcttgt gaattttcta aataacaggt ggatatcatc
      361 ttttcgttct ctcaaggcgg gaacggagat aggcacagtg ttcaagcggt aaaacagatc
      421 ttcccggaat ttgccttcag caataccttt atgcaggtct acattcgtag ctgccactac
      481 ccttacatca gttttttgca ccttcgatga ccctacccga ataaactcac ctgcctccaa
      541 cacacgtaat agtcgcgcct gtgtgccatg cggcaattca cctacttcgt ccaaaaaaat
      601 agtaccgccg tttactgttt cgaaatagcc tttcctgtcg tcaacagcat tagtaaacga
      661 gcctttcaca tgaccgaaaa gctcagaatc aatggtacct tccgggatag caccgcagtt
      721 cacagcaata aagggaccat gcttgcgtgt gctgtattga tggatgattt gagaaaaaat
      781 ctctttcccc accccacttt ctccggtaat gagcaccgta agattcgttg gtgctacacg
      841 agctgcaata ctcaatgcgt aattgagtcc cggggcattg ccaataatgc caaatcgctg
      901 ttttatggat tgaatttcgt ctgccatggt ttttaaactg ctttatgcat gcctctgccc
      961 accatttcgc caataagcgt tgctgatgta gctgaagtga cacgcacctg cacatagtcg
     1021 ccgggattta catctgtagc cgggaaaacg accattttat tttgatcatt gcgaccgcac
     1081 atatcgagtg ccgaacgttt actaggcttt tcaacgagga ctgtgaatgt ctttccgata
     1141 tccgcttgat ttcttgcatg cgaatgcttc gtttgcagcg cgatgatctc gttcaatctt
     1201 ctgattttgg tctcgtgcgg aatatcatcg gtatacttcc ttgcagcaag ggttcccggc
     1261 cgctcactgt agaaaaacat gtaagccagg tcgtattgaa ccatttccat caaactcaaa
     1321 gtatcctgat gctcttcctc agtctcgctg caaaatccgg taattacatc cgtagaaata
     1381 ccacaatccg gcactatgtt gcgaattgca ttgattctat cgaggtacca ttcacggtcg
     1441 taggttctgt tcataagctc cagtaccctg gagttacccg attgcaccgg taggtggata
     1501 taattacaga tattatcgaa tcgcgccatg gtatgaagca cctcatcagt aatatctttt
     1561 ggatgcgagg tagagaagcg cacgcgaagc gttgaatcga cggaggcaac cattgccatc
     1621 aattcggcaa aattcaccac tttccctgtt tcgggatttt cccatttata cgaatcgaca
     1681 ttctgcccaa ggagggtt
//
LOCUS       DAOQHY010000341         1682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_60507_length_1682_cov_7.672403, whole genome shotgun sequence.
ACCESSION   DAOQHY010000341 DAOQHY010000000
VERSION     DAOQHY010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60507_length_1682_cov_7.672403"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PLL28_15300"
     CDS             complement(<1..212)
                     /locus_tag="PLL28_15300"
                     /inference="COORDINATES: protein motif:HMM:NF025057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HNF70746.1"
                     /translation="MSDDLSQKPIIIKRIKKVSGGHHGGAWKIAYADFVTAMMAFFLL
                     MWLLGSTTQGDKEGIAEYFKTPLKIAL"
     gene            complement(249..1109)
                     /gene="motA"
                     /locus_tag="PLL28_15305"
     CDS             complement(249..1109)
                     /gene="motA"
                     /locus_tag="PLL28_15305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HNF70747.1"
                     /translation="MLVSVGYLIVIVSVFGGFALAGGHLVSLFQPVELLMIGGGALGA
                     FFVGNSGKAIKATLKALPHVFKGAKYTKDVYMELMTLLYEILGKIRKEGLMAIEADVE
                     DPHKSPLFSKYPKILSDHHVIEFITDYLRLMVGGNLNPLEIENLMDGEIETHHQEEEV
                     PIHAIAKLGDGLPAFGIVAAVMGVVHTMESVGIPPAELGKLIAAALVGTFLGILLAYG
                     FVGPLASKLEQRLHESSKLLECIKVTLLANLNGYAPALAIEFGRKVLYSTERPSFAEL
                     EKHVKQSKAK"
     gene            complement(1294..1682)
                     /locus_tag="PLL28_15310"
                     /pseudo
     CDS             complement(1294..1682)
                     /locus_tag="PLL28_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611289.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          484 a          377 c          307 g          514 t
ORIGIN      
        1 agtgctatct ttaatggtgt tttaaaatac tctgcgatac cttctttatc gccttgagtg
       61 gtcgagccta gtagccacat taatagaaag aaagccatca ttgctgtaac aaaatctgca
      121 tatgctatct tccatgcacc accgtgatgc cccccactta ctttcttgat gcgcttgata
      181 attataggct tttgtgaaag atcgtcagac atcagtcatc tctttgaagt tttatagggg
      241 attcgctatc acttagcctt gctttgcttc acatgtttct ctagctctgc aaacgaaggt
      301 ctttctgttg aatataaaac ctttcttcca aattcaattg caagagctgg cgcgtaacca
      361 ttcagattag ctaatagcgt aaccttaatg cattctagca acttacttga ttcatgaagg
      421 cgttgttcta atttgctagc aagaggacca acaaatccat atgcaagtaa aattcctaga
      481 aaggtcccga ccagagcggc tgcaatgagc ttccctagct ctgccggggg aattccaaca
      541 gactccatgg tgtgtacaac gcccatgacg gccgcgacaa ttccaaatgc tggaagccca
      601 tcacctagtt ttgcaattgc atgaatagga acttcttcct cttggtgatg cgtttcaatt
      661 tccccatcca taagattttc aatttctaat ggatttagat ttccaccaac catcaagcgc
      721 aaataatctg taataaattc aataacatgg tgatctgata aaatttttgg atatttagag
      781 aataatggac ttttgtgagg atcttcaaca tcggcttcta tagccattag tccttctttg
      841 cgtatttttc ccaatatctc ataaagcaga gtcatcagct ccatatatac atctttagta
      901 tatttggcac ctttaaaaac atgaggtagt gctttcaggg ttgccttaat agctttacct
      961 gaatttccaa caaaaaacgc tccaagagcc cctcccccaa tcatcaacag ttcaactggt
     1021 tgaaataatg atactaaatg tcctccagcg agggcaaagc cgccaaaaac agatactatt
     1081 acaataagat aaccaaccga tactaacatt tgaagaatcc tggaaatttt caattgtaac
     1141 ggctaatgtg cctgctactt tagcagcaag actcaaacag agggcgctaa agaccaattg
     1201 gtaattgcct ctcataaaat aaagctcgag cgattctgcc ttctttgtca ccagtggagt
     1261 tgtgacctgg tcaagtaaaa ccggacacat cagttaagct gcttttatcg attttactgc
     1321 ttctgcttca tattgattta ggctcttgta attcaggtat gaatgccgtt tggttgttat
     1381 agaacacaac gataattacg attgccggat acagctagcc ttcttgtgtc cagatgcagg
     1441 tgatatcgta aacataaatt tgatccggct ggcaacatca aattgccggt ttaatcggtt
     1501 ttcaaatacc ggcaacggat gatcactgtc cgtcaccact ttgtatttct ttcgatacct
     1561 gacagtcacg ccaacctcgt gcattaattt ccttgtcttc caacggctaa ttgaatatcc
     1621 caaggtattt agtcggtcct gcaaaattaa cttaaccgtg gcggaggttt tgtatacaag
     1681 aa
//
LOCUS       DAOQHY010000342         1607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_65797_length_1607_cov_8.925258, whole genome shotgun sequence.
ACCESSION   DAOQHY010000342 DAOQHY010000000
VERSION     DAOQHY010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65797_length_1607_cov_8.925258"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1607
                     /locus_tag="PLL28_15315"
     CDS             <1..>1607
                     /locus_tag="PLL28_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70748.1"
                     /translation="DLSPIPYPKDYVEGETCYFNYESGDKVNLAYWNADTYVAKFDAS
                     DEMEWEQTYPTTTEPYDEFVMFPDDIKRMECVYHIVEAPDGGLVVAGNNSKNFDDDLV
                     IKLYSDCNRNVSYYTGSGDINTEGISLIGTDETWDSNIKVRGIIEIQDDATLTITNEA
                     VIQFADSKADNTPTYILVDRGGRLIIEDGATLTGMTECGTMWDGIYVKGTYGTNHPTP
                     ASLLGSGYFVTGDAHGLVKISGGATIEHARNGVTLGLPATMEDYPDYGGGILIADNAN
                     FVNNTIDVMMWPFDKNNVSFIRHCNFATNAALPDPVEIPEAHIKMIQVKNVNIRGNVF
                     ENSTAPTVYTTSQRGMGILGYNATFKANDNPNGFATTGGTGSDPNYFTDLYYGVYAAQ
                     FTTLSTNILVQGNNFSENGNGIYIGGATLPEVNFNTITLPSKSNYGIYLDGCDAYGVE
                     ENNITGAGADLLGTQIGIVINESGNGSNWIYRNNFSALKYGVRMQGNNGDTDDGLEIK
                     CNTFEEGKYDISLISGATTATMKKNQGA"
BASE COUNT          513 a          283 c          369 g          442 t
ORIGIN      
        1 gatttatcac ctatcccata tccaaaggat tatgttgagg gcgaaacttg ttacttcaac
       61 tatgaatcag gtgacaaggt taatcttgcg tattggaacg ctgataccta tgtagcaaag
      121 tttgatgcat cagatgaaat ggaatgggaa caaacttatc ctactactac agagccttat
      181 gacgagtttg ttatgtttcc ggatgatatc aagcggatgg agtgcgtata tcatattgtt
      241 gaagcccctg atggtggttt agtagttgcc ggaaacaatt ctaagaattt tgatgatgat
      301 ttagtaataa aactgtatag cgactgtaac cgaaatgttt cctattatac cggatccggt
      361 gacattaata ctgaaggaat ctcgttaata ggtacggatg aaacatggga ctcaaacatc
      421 aaagttcgag gcattatcga aatacaagat gacgccacat taaccataac caatgaagct
      481 gtcattcaat ttgctgattc caaagcggat aataccccta cgtacattct ggtagataga
      541 ggggggcgat taatcattga agatggtgcc acacttaccg gtatgactga gtgcggcact
      601 atgtgggatg gcatttatgt aaaaggcact tatggtacta accatcctac gcccgcaagt
      661 ttgcttggat ctggttactt tgtaacaggg gatgcacatg gattggtgaa aatttcaggc
      721 ggagctacaa ttgaacacgc acgaaatggc gttacacttg gtctccctgc aacaatggaa
      781 gattatcccg attatggagg cggtatatta attgcagaca acgccaattt tgtgaataat
      841 acgatagatg taatgatgtg gccgtttgat aaaaataatg tcagctttat caggcattgc
      901 aattttgcaa caaatgctgc attgcccgat ccggttgaaa ttccggaagc acatatcaaa
      961 atgatacagg tgaaaaatgt caacatcaga ggcaatgtgt ttgaaaactc gacggcgcca
     1021 acagtttata caacttcaca aagaggtatg ggtatattag ggtataatgc tacttttaag
     1081 gcaaatgata atccgaatgg atttgccaca acaggcggca caggtagcga tccgaactat
     1141 tttacagatt tgtattatgg tgtttatgcc gctcagttta ccacactaag tacaaacatc
     1201 cttgttcagg gaaacaattt tagcgagaat ggcaatggca tttacattgg cggagccaca
     1261 ttgcctgagg tgaattttaa tacgatcaca ttaccctcaa aaagcaatta tggtatttat
     1321 ctggatggat gtgatgctta tggggtagag gaaaacaata ttacaggcgc aggtgcggat
     1381 ttattaggga cacaaattgg catagttatt aacgaaagtg gcaacggttc caactggata
     1441 tatcgcaata atttttctgc attaaaatac ggcgtacgca tgcagggaaa caatggcgac
     1501 actgacgatg gtctggagat caagtgcaat actttcgaag aaggtaaata tgatatatca
     1561 ttgatatcag gtgctacaac ggcaacgatg aaaaaaaatc agggagc
//
LOCUS       DAOQHY010000343         1602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_66175_length_1602_cov_7.179056, whole genome shotgun sequence.
ACCESSION   DAOQHY010000343 DAOQHY010000000
VERSION     DAOQHY010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66175_length_1602_cov_7.179056"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            336..>1602
                     /gene="rho"
                     /locus_tag="PLL28_15320"
     CDS             336..>1602
                     /gene="rho"
                     /locus_tag="PLL28_15320"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNF70749.1"
                     /translation="MYTEEQLSDMVVPELREIAEGLSVANVKKLGKSELISAIASAQA
                     ANGKGEKQDRKRVLRPRKKKDTPVDENVKQLEIPDVVEEEAPIEIPEKTEAPVAPVAP
                     VVEQIEKPVREEQPQHQHQHQYPQKKREPRFNIDIDGAVPGEGVLEMMPDGYGFLRSA
                     DYNYLSSPDDVYVSPSQIKLFGLKTGDWVSGFVRPPKEGEKYFALLRVETINGRDPKE
                     IRDRVPFDYLTPLFPDEKLNLIYNAGDYSTRVIDLFTPIGKGQRGMIVAQPKVGKTYL
                     LKSIANAIAQNHPEIYLIVLLIDERPEEVTDMERSVNAEVIASTFDEPAEKHVKVSSI
                     VLQKAKRLVECGQDVVILLDSITRLARAHNTVAPSSGKVLSGGVEANAMQKPKQFFGA
                     ARNIENGGSLTILATALIDTGSKMDEVIFE"
BASE COUNT          481 a          313 c          363 g          445 t
ORIGIN      
        1 atatcatact tatttcgttt tgacagtcgt tcttttgctt cagatgtatg tgtgcggagg
       61 tagccgatct cgatcattcc ctaaatttta acgcaaatat aaggctactg tttggcgcaa
      121 gggctttgag ggcttgcaat attcaaaata tcaccttatt attgcaccgt aaagtccatt
      181 taatcgtaag tcggacacac atcaccaccg gttttttcag ccaccttttc ctaattagtt
      241 agcttatcgg gttctgtttt accggcatca atacacacat gtttttagta agtcagtata
      301 tccattacat tattatttaa tcttattcat ctagtatgta cactgaggaa cagttgagcg
      361 acatggtcgt tcctgaactg agagagatcg ccgaaggatt gtctgttgcg aatgtgaaga
      421 agctcggtaa atctgaatta atttctgcaa ttgcatctgc tcaggcagca aatggcaaag
      481 gcgagaaaca agatcgcaag cgcgttttgc gtccgcgcaa gaaaaaagat acgcctgttg
      541 acgaaaatgt taaacaactc gaaattccgg atgttgttga agaggaagcg ccgatagaaa
      601 ttccggaaaa aacagaagca cctgtagctc ccgttgcacc ggttgttgag cagatagaaa
      661 aacctgttcg cgaagaacaa ccacaacatc aacaccaaca tcaatatccg caaaaaaagc
      721 gcgaaccaag atttaatatc gacatcgatg gcgccgtacc cggtgaaggt gtattggaaa
      781 tgatgcccga tggctatgga tttttgagaa gcgcagatta taattattta tcatcacccg
      841 atgatgtata tgtttctcca tcacaaataa aattattcgg attaaagacc ggcgactggg
      901 tttcgggctt tgtccgccct ccgaaagaag gtgaaaaata ttttgcttta ttgcgtgttg
      961 aaacaattaa tggtcgcgac ccgaaagaaa ttcgcgatcg tgtgccattc gattatctta
     1021 caccattatt tcctgatgag aaattaaatc tcatttataa tgcaggagat tacagcactc
     1081 gcgtaattga tttatttacg ccgataggaa aaggtcagcg cggaatgatc gttgcgcaac
     1141 ctaaagtagg taagacttac ttattaaaaa gtattgcgaa tgcgatagcg caaaatcatc
     1201 ctgaaattta tctgatcgta ttattgatcg atgagcgtcc ggaagaggtt actgatatgg
     1261 agcgcagtgt gaatgcggaa gtaattgcat cgacatttga tgaacctgca gagaaacacg
     1321 taaaggtgag cagtatcgta ttgcagaaag cgaaacgatt ggtagaatgc ggacaggatg
     1381 ttgtaatcct gctcgattcg atcacgcgct tggctcgtgc acataatacg gttgctcctt
     1441 cgagtggtaa agtgttatcg ggaggtgtgg aagcaaacgc catgcaaaaa ccaaaacaat
     1501 tctttggcgc tgcgcgaaat atcgaaaacg gtggttcgct taccattctt gctactgcat
     1561 taatcgacac cggttcgaaa atggatgagg tgatttttga gg
//
LOCUS       DAOQHY010000344         1596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_66651_length_1596_cov_4.888384, whole genome shotgun sequence.
ACCESSION   DAOQHY010000344 DAOQHY010000000
VERSION     DAOQHY010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66651_length_1596_cov_4.888384"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="PLL28_15325"
     CDS             <1..138
                     /locus_tag="PLL28_15325"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HNF70750.1"
                     /translation="LRDYEGYSYEEIGEVLNLSESQVKVYIFRARQTMKEYLVSIHYV
                     I"
     gene            135..1007
                     /locus_tag="PLL28_15330"
     CDS             135..1007
                     /locus_tag="PLL28_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70751.1"
                     /translation="MKITMQNYEEWMIDYIEGNLTPEQEKAMHEFLAFHPELRAELEL
                     FNHTRLTPETVAFPDKAALKHEEPAGRVITMFSWVRYAAAAAAVVAVFIGIRYFGGEQ
                     QQQGIQRYAQEQYDSNSINRNTTKTPIIESPVKQQQQEYAQTTTPEKNNIKSLPKEEV
                     KEQVKREINPIQGIDFASVDQIKSKQKDASLRDMMQQEPENEMVAMTDNQIKTGNAMN
                     STVISLNDNSTVVDWWVDAMAIGNEVGGVVNGVREAEINPFKKKEAGAQEVVKTKNIN
                     IPGFSYYSRKKTNH"
     gene            1026..>1596
                     /locus_tag="PLL28_15335"
     CDS             1026..>1596
                     /locus_tag="PLL28_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70752.1"
                     /translation="MKRIIYLVISMLAPVAMFAQVPTPPPPPVVPVEGTIKVEDEKVT
                     ITTVKGDIFIIDEFGVRKNGIMIAGDSEALREEIEDLQDEIDDIREESASLAPGSDAY
                     NDLMDEVSDLEDELTALSPESMYPDTLPGDSTALTIGNWRLIVKETGDDDDVDVTFGK
                     VPAIEEINDHYVDPFQTEWFLFDMGYNTYL"
BASE COUNT          531 a          284 c          367 g          414 t
ORIGIN      
        1 cttcgcgatt atgaaggata cagttatgag gagataggag aggtgctgaa tttgagcgag
       61 agccaagtga aagtgtacat tttcagggca aggcaaacaa tgaaagaata tttggtaagc
      121 atacattacg ttatatgaag atcacaatgc agaattacga ggaatggatg atcgattaca
      181 tagaaggtaa tctgacaccg gagcaggaaa aagccatgca tgagtttttg gctttccatc
      241 ctgaactacg tgctgagctg gaattgttta atcatacacg ccttacacct gaaactgttg
      301 ccttccctga taaggctgcg cttaagcatg aagagcccgc aggacgcgtg attaccatgt
      361 tcagttgggt gcgctatgct gctgccgccg ctgcagtagt tgcagttttc atcggcattc
      421 gatatttcgg tggtgaacaa cagcaacaag gcattcaacg ctatgctcag gagcagtatg
      481 acagcaattc gatcaatcgc aataccacaa aaacaccgat tatagaatcg ccggttaaac
      541 aacagcagca agaatatgcg caaacaacaa caccggaaaa aaataatatt aaaagccttc
      601 ctaaagagga agttaaagaa caagtaaaaa gggaaattaa cccgatacaa ggaattgatt
      661 ttgcgtcggt tgatcagatt aaaagcaagc aaaaagatgc aagcttgcgc gatatgatgc
      721 aacaggaacc tgaaaatgaa atggttgcca tgaccgataa tcaaatcaaa acaggcaacg
      781 caatgaatag tactgttatt tcattaaatg ataacagcac tgttgtcgac tggtgggtag
      841 atgccatggc cattggaaat gaagtaggag gagtagtaaa tggtgttcgc gaagctgaga
      901 tcaatccctt taaaaagaaa gaagcaggcg cacaagaagt agtgaaaaca aaaaatatca
      961 atatacccgg attttcttat tacagtcgta aaaaaacaaa tcattaatcg cataaatcgt
     1021 atattatgaa aaggattatt tatttagtta taagcatgct ggctcctgtt gccatgttcg
     1081 ctcaggtgcc cactccacct cctccaccgg ttgtgcctgt ggaaggaact atcaaagttg
     1141 aagatgagaa ggttactatt acaaccgtga aaggcgacat ctttattatc gatgaatttg
     1201 gtgtgcgtaa aaatggaatt atgatagccg gtgattctga agcactgcgt gaagaaattg
     1261 aagatttgca agatgagatt gatgatatcc gtgaagaatc tgcgtcattg gctcctggtt
     1321 cagatgctta taatgatctg atggatgaag tttctgatct tgaagatgaa ctgaccgcac
     1381 tcagccctga atccatgtat ccggatactt tgccgggtga ttcaaccgca ttgacaattg
     1441 gtaactggcg acttatcgtt aaagaaacag gtgatgatga tgatgtagat gttacattcg
     1501 gtaaagtgcc cgctattgaa gaaattaatg accattatgt ggatccgttt caaactgaat
     1561 ggttcttatt tgatatggga tataatacct atttaa
//
LOCUS       DAOQHY010000345         1497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_75160_length_1497_cov_3.179612, whole genome shotgun sequence.
ACCESSION   DAOQHY010000345 DAOQHY010000000
VERSION     DAOQHY010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75160_length_1497_cov_3.179612"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..>1497)
                     /locus_tag="PLL28_15340"
     CDS             complement(182..>1497)
                     /locus_tag="PLL28_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70753.1"
                     /translation="MRVILLLFIASLSTFSFAQTWSYVGPCCIESDVDGLSSAGSEDL
                     DFLSDGTPVVSFFRQTGGATIGKVRQLNGGAWEQVGGDLPAAGTISAIDLETHGGNIY
                     VSILTGTSQISVYTFDGTDWMQVGSTVTGNLAYDFIVDNDGTLYLFTTLDQSIRKFNG
                     TDWEVVLNFGVSSFLQWTSDQSIVIDSNNDMYFYQSFLNGATFIFENYVYKFNGTTTE
                     QVGNMVFDGFGSVGKLGVDGDDNIYAQYSSDGTNKITKLEGTNWNQLLDTTNAANGIF
                     GFLYAFNSDNKMLLSTFTQVYYADGYVALPELPLTGLPIAINNMVLAPDGNIYISFGE
                     IPAGTGSNFSVMVLDEGVAIHDLQNNAIQVYPNPSSGECNVSGLSSGDILQLFDFTGR
                     ILYSEVFYGSGMSTFHVNAAPGSYYLFIQRGSERFGKCVQITGIK"
BASE COUNT          484 a          313 c          275 g          425 t
ORIGIN      
        1 ttgagataca aggtaagaaa aaaatcacgc cgcaacaatt gttatttatt ccatgtatat
       61 aggattgttt atatacaacg cacaaaatca tgtgtagtcg gcatatttaa ctgccaaaga
      121 ccacacatga tttatccaca gtttaaatat taatattccg aggaataaat taatgaatga
      181 tttatttaat gccggtaatt tgaacacatt ttccaaaacg ttcagagccg cgttgaatga
      241 ataaataata ggagccggga gctgcgttaa catgaaaagt tgacattcca cttccataga
      301 acacttctga atataatatg cgaccggtaa aatcgaacaa ttggagtatg tcgccggatg
      361 acaagccgga aacattacat tcacctgatg atggatttgg ataaacttga atcgcattat
      421 tttgtaaatc gtgaatagca acgccttcgt ctaacaccat tacactaaaa ttcgaacctg
      481 ttccggcagg gatttcaccg aagctgatat agatattccc atcaggtgcc aataccatat
      541 tatttatggc tatgggtaat ccggttagcg gtaattcagg caatgccaca tatccatcgg
      601 cataatatac ttgtgtgaag gtgctgagca gcattttatt atcgctattg aaagcatata
      661 aaaaaccgaa aattccattt gctgcattcg tagtatcaag caattgattc caatttgttc
      721 cttccagttt ggtgatttta tttgtgccgt cagaactata ttgtgcataa atattatcat
      781 cgccatccac acctaatttt cctacagatc caaatccatc aaataccata ttgccgactt
      841 gttcagttgt agtgccattg aatttgtaaa cataattttc aaaaatgaat gtggcgccat
      901 ttaaaaatga ttgatagaaa tacatgtcgt tattactgtc aattacgata ctttggtcag
      961 aagtccactg aagaaaagaa gacacaccaa aattcagcac cacttcccaa tcagttccat
     1021 tgaattttct gatcgactga tccagcgttg taaataaata caatgtgcca tcattatcta
     1081 caatgaaatc atatgcaaga ttaccggtca cggtagatcc cacctgcatc caatctgttc
     1141 catcgaaagt atacacgcta atttgcgagg taccggtgag tatggaaaca taaatattgc
     1201 ctccgtgtgt ttcaaggtcg atagcagaga tagttccggc agctgggaga tcgccaccta
     1261 cttgttccca ggcaccccca tttaattgac gcactttccc gatggtagcg ccccctgttt
     1321 gacgaaaaaa gctcactacg ggcgttccgt cgctcaaaaa atcaaggtct tcagagcctg
     1381 cagacgatag tccgtctaca tccgattcaa tacagcaagg cccgacatag ctccaggttt
     1441 gtgcgaaaga aaatgtactt agtgaagcaa taaatagcag taaaatgaca cgcatag
//
LOCUS       DAOQHY010000346         1460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_78880_length_1460_cov_5.594306, whole genome shotgun sequence.
ACCESSION   DAOQHY010000346 DAOQHY010000000
VERSION     DAOQHY010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78880_length_1460_cov_5.594306"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="PLL28_15345"
     CDS             <1..872
                     /locus_tag="PLL28_15345"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNF70754.1"
                     /translation="IGSLNFEVISIGMAANPLMIFGFVLIFAGFAFKLSAAPFHLWTA
                     DVYEGAPVAVTSYLSVISKGAIVFVFITILYRLFQNIFDTWYQILFLIAVLTMVIGNL
                     FAMRQQNLKRFLAFSSIAQVGFILIGIGGNSSEGLTASVYFVLIYIFSNLAAFGVISL
                     VSTLTGKESISDYKGFYKTNPLLAWVLTLALFSLAGIPPTAGFFGKFFLLMAGAAQDN
                     YPLVIIAALNMIVSLYYYLRVVKAMFMEENADPIQKLKTGVSPALALIICVAGLLLTG
                     LIGGAYEYISSIV"
     gene            882..1238
                     /locus_tag="PLL28_15350"
     CDS             882..1238
                     /locus_tag="PLL28_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNF70755.1"
                     /translation="MAINQNHLFDDLDGIKCAVVEKNVSPERVAFLRSLLEYNRYQVV
                     VVPSPPAKGAAPVPEGETPPPPTSFTLGVSDVTFNAMNAIFGRLLRTPDGHVVTLKYW
                     QQKDAVAHDTVPYFYK"
BASE COUNT          386 a          269 c          327 g          478 t
ORIGIN      
        1 caatcggaag tttgaatttt gaagtaatca gtattggcat ggcggcaaat ccgctcatga
       61 tttttggttt tgtgctgata ttcgcgggct ttgcgtttaa gttatctgcc gcgccatttc
      121 atctatggac agcagatgtg tatgaaggcg ccccggtggc agtaacttca tatttaagtg
      181 tgatttcgaa aggtgcaatt gtatttgtat ttattaccat actttatcgt ttattccaaa
      241 acatattcga cacctggtat cagatacttt tccttattgc ggtgcttacc atggtgatag
      301 gaaatttatt tgccatgcga cagcagaatc ttaaacggtt cctggcattc tcttcaatag
      361 cgcaagttgg atttattttg ataggtatcg gcggtaattc ttcagaaggg ttgacagcat
      421 cggtatactt tgtattaatt tacattttca gcaatttggc tgcattcggc gtgatttctt
      481 tggtgagcac attaacggga aaagaaagca tttcagatta caaaggattt tacaaaacga
      541 atcccttact tgcatgggtg ctcacattag cattattttc tttggccggc attccgccga
      601 cagctggatt ttttggtaag tttttcctat tgatggcagg tgctgcgcag gataattatc
      661 cgttggtgat cattgctgca ttgaacatga ttgtttcgct ctattattat ctgagggttg
      721 taaaagcaat gttcatggaa gaaaatgcag atcccattca aaagttaaaa acaggagttt
      781 ctccggcatt agcactcatc atttgtgttg caggtctttt gctgacaggt ttgataggcg
      841 gtgcttacga atatatttca tcaatagttt aatttgctcc tatggctatt aatcagaatc
      901 atttattcga cgatctcgac ggtataaaat gtgctgttgt agaaaaaaat gtatcgcctg
      961 agcgcgtggc atttcttcgt tcgcttctgg aatataatcg ctatcaggtg gtagtagtac
     1021 cttcacctcc cgcaaaagga gcagcacctg ttcccgaagg ggaaacccct cctccaccga
     1081 caagtttcac cttaggagta agcgacgtca ccttcaatgc catgaatgcc attttcggtc
     1141 gcctcttaag aactcccgac ggccatgtgg ttaccttaaa atactggcaa caaaaagatg
     1201 cggtggcaca tgatacagtg ccttattttt ataagtagcg agaattttgt tgttaagggt
     1261 gggtattgaa gattcgaagg tttgaagaat ttaagattcg aaggttcgaa gatttgaaga
     1321 ttcgaaggtt tgaagaattg aaggttggaa gttgaaagat tgaaggttgt atttcactcc
     1381 cactactcac taccaaatcg tgactcgtga atcgtgagtc gtgaatcgtg aatttttacc
     1441 tgatacttga tacttgatac
//
LOCUS       DAOQHY010000347         1459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_79023_length_1459_cov_3.032051, whole genome shotgun sequence.
ACCESSION   DAOQHY010000347 DAOQHY010000000
VERSION     DAOQHY010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79023_length_1459_cov_3.032051"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1385
                     /locus_tag="PLL28_15355"
     CDS             <1..1385
                     /locus_tag="PLL28_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HNF70756.1"
                     /translation="GMPGLSGLETLQLIKEKRPAIPIVMITKNETEGLMEEAIGSQIS
                     DYLIKPVKSQQILLTLKKLIDNKRLVTEKTDSNYQKEFQKLFFSLQDANDSEQWFDIY
                     KKLVHWEIQLDKTGTAGMKEVLTSQKAEANVEFGKFIDKNYLSWMAGKGKRPVMSHTL
                     MQEKVFPLLQDKVPTFFILIDNLRFDQWKVIEPLISESFKLKSEDYFFSILPTATQYA
                     RNAIFAGLTPAEIEKRLPSLWLNDEEEGGKNMQESEFLKDNIVRNKMNVKYSYHKILN
                     YSEGKNLEDNILNLLNNDFNAIVYNFVDMLSHARTEMEVLKELASDETAYRSLTRSWF
                     DHSPLHDALRKLSSKKVRIIITTDHGTIRVHTPSKVVADRNTTTNLRYKSGKNLQYNK
                     KDVFEVKVPAEAGLPKQHVTSSFIFAKEDAFFAYPNNYNYYVNYFKNTFQHGGISLEE
                     MIIPFAVFES"
BASE COUNT          482 a          260 c          325 g          392 t
ORIGIN      
        1 aaggaatgcc gggcttgtcg ggattggaga ctttacaact gatcaaagaa aagcgccctg
       61 ccataccgat tgtgatgatt accaagaatg aaacggaggg tttgatggaa gaggccattg
      121 gctcgcagat cagtgattac cttattaaac ctgtgaaatc gcaacagata ttactgacgc
      181 tcaaaaagtt gatagacaac aagcgattgg taaccgagaa aaccgattca aattatcaga
      241 aggaatttca aaaactattt ttttcactgc aagatgccaa cgattcggag cagtggtttg
      301 atatttataa aaaactcgtt cactgggaaa ttcaactcga taaaaccggc actgcgggaa
      361 tgaaagaagt gctgacttct cagaaagcag aagccaatgt tgaattcggg aaattcatcg
      421 ataagaatta tttgtcgtgg atggcaggaa aaggcaagcg tccggtaatg tcgcacacgc
      481 tgatgcagga aaaagtgttc ccattattac aggataaggt gcctacattt tttattctta
      541 tcgacaattt gcgcttcgac caatggaagg tgatagaacc attgatcagt gaatctttca
      601 aattaaaatc ggaagattat ttctttagca ttttaccaac agccacacag tatgcacgta
      661 atgccatttt cgcgggactg acgcctgctg aaattgaaaa acgattgcct tcattgtggt
      721 tgaatgatga ggaagaggga ggaaagaata tgcaggaatc tgaattcctg aaagacaata
      781 ttgtgcgcaa taaaatgaat gtaaaataca gttatcacaa aattctcaac tattccgaag
      841 gaaagaatct tgaagataat attttgaatt tattgaataa cgattttaat gctattgttt
      901 acaattttgt cgacatgctg tcgcatgcgc gcacagaaat ggaagtatta aaagaactcg
      961 ccagcgatga aacagcatat cgctcgctca cccgttcatg gttcgaccac tcgccattgc
     1021 acgatgcttt gcgcaaattg agctcgaaga aagtgcgcat tatcatcact actgatcatg
     1081 gcactatcag agtgcatact ccgagcaagg ttgtagcaga tagaaacacc accacgaatt
     1141 tgcgttacaa gagtggaaag aatttgcaat acaataaaaa agacgtgttc gaagtaaagg
     1201 tgcctgcaga agccggttta ccaaagcaac acgtaaccag ctcctttata tttgcaaagg
     1261 aagatgcttt ttttgcttat ccgaataact acaattacta tgtaaactat ttcaaaaata
     1321 cgttccaaca tggaggtatt agtttggagg agatgattat tccgtttgcg gtgtttgaga
     1381 gttgagggtt gttggatgga agattcaggt atcaggtatc aggtatcaag tatcaggtat
     1441 caggtatcaa gtatcaggt
//
LOCUS       DAOQHY010000348         1335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_93653_length_1335_cov_5.670312, whole genome shotgun sequence.
ACCESSION   DAOQHY010000348 DAOQHY010000000
VERSION     DAOQHY010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93653_length_1335_cov_5.670312"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PLL28_15360"
     CDS             <1..685
                     /locus_tag="PLL28_15360"
                     /inference="COORDINATES: protein motif:HMM:NF020295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNF70757.1"
                     /translation="VSAALREPRIFFQFNKLIPYPNGSYLCILKDYNMSNFIISIYTE
                     ATPNPDSLKFVMNKMLLTGRSVDFERGDDVSYAPLAEAIFNEFDFAKGVFIMNNFVTV
                     RKQGEVDWYEVKSKVSEFIKNFVSEGNTVVGEIPEIDNISGEMDGDAVVAKIRQMLDQ
                     YVKPAVEMDGGAIQFKSFDNGIVTLIMQGSCSGCPSSTVTLKSGIEGLLKRMVPEVKE
                     VVAESMEIM"
     gene            complement(781..>1335)
                     /locus_tag="PLL28_15365"
     CDS             complement(781..>1335)
                     /locus_tag="PLL28_15365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HNF70758.1"
                     /translation="WAEDVIMSMNEHIKFIDIEHRGFLMLDVTKEKVQGDYYFINNIE
                     TPDTLYNYDDSWMILNGNNWLYHADTATVRAMDRMVPLAPACPAAPDTATVNIIAPDV
                     VILGVYPNPVHEELTVQFSLQQRSTMLLTIRDMQKQVIRHEVITADAGLHVIKLSVDK
                     LSSGTYFLELKQGENTLVRTFVKR"
BASE COUNT          390 a          307 c          265 g          373 t
ORIGIN      
        1 ggtctctgcg gctctgcgtg aaccccgcat tttttttcag tttaacaaac ttattcccta
       61 tccaaatggt tcttaccttt gcatactcaa agactataac atgagcaatt tcatcatcag
      121 catatatacc gaggcaacac caaacccgga ttccctcaaa ttcgtcatga acaaaatgct
      181 cctcaccggc cgcagtgtcg acttcgagcg cggtgatgac gtaagctacg cccctttggc
      241 agaagctatt ttcaacgagt tcgactttgc taagggcgta ttcatcatga acaacttcgt
      301 taccgtacgc aaacagggcg aggttgattg gtatgaagtg aaatcaaaag tgagcgagtt
      361 tatcaaaaat tttgtttctg aaggaaatac cgttgttggt gaaattcctg aaatcgacaa
      421 tatcagcggc gaaatggacg gcgatgctgt tgttgccaag atccgccaaa tgctcgatca
      481 atatgtaaaa cctgcagttg aaatggacgg cggcgctatc caatttaaat catttgataa
      541 tggcatcgtt acattaatca tgcaaggatc ttgcagtgga tgcccatcat caactgttac
      601 actaaaatca ggcatcgaag gcttgctcaa gcgtatggtt cctgaagtga aagaagtggt
      661 agcggaaagc atggagataa tgtgagaatg tgggaatgtg ggaatgtgag aatgtgggaa
      721 tacatgtatt caaacctctg cgactctgcg cctctgcgtg aaaaacaaat agaataatcc
      781 ctatcgctta acaaaagtcc ttaccagcgt attctcccct tgttttaatt ccaggaagta
      841 ggtgcctgat gataatttat ctactgataa ttttatcaca tgtaatcccg catctgcagt
      901 aattacttca tgccttatca cctgcttttg catatcacga atcgtgagca acatggttga
      961 tcgttgttgc aacgaaaatt gcaccgtcaa ctcctcatgc acaggatttg gatacacccc
     1021 caatatcacc acatccggtg cgataatatt tactgtggcc gtatcaggtg cagcaggaca
     1081 agcaggtgcc aatggaacca tacgatccat cgcacgcacc gttgccgtat ctgcatgata
     1141 caaccagtta ttcccattca atatcatcca actatcatca taattatata gtgtatcagg
     1201 cgtttcgata ttattgatga aataataatc accctgcacc ttttccttcg ttacatccaa
     1261 catcaaaaat ccgcgatgtt caatgtcgat gaatttaata tgctcattca tcgacatgat
     1321 cacatcctct gccca
//
LOCUS       DAOQHY010000349         1333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_93929_length_1333_cov_4.406886, whole genome shotgun sequence.
ACCESSION   DAOQHY010000349 DAOQHY010000000
VERSION     DAOQHY010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93929_length_1333_cov_4.406886"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(290..>1333)
                     /locus_tag="PLL28_15370"
     CDS             complement(290..>1333)
                     /locus_tag="PLL28_15370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HNF70759.1"
                     /translation="TNMSVEICNGDSYLLPDGTTTAIAGFYSFNLPSVHGCDSTVNIT
                     LTIKDPVEITQNIQRCEGDVYMLPDGNAATTSGIYTTVFTLPSGCDSTIITHLNFATP
                     IIPEMVLPEQICLEAEPIHIETFPEGGIYSAGIGYGNFDPQVFGLGTHTVTYVYTSAA
                     GCTAETTSTITVTSNYANAGPDAVIQQNTTTTLHGDAGGDYTWMPALFLACNTCQETV
                     AMPPVNTMYTLTSVDAVGCIATDSVMISVTDAESDAVWFPNTFTPNGDGMNDYFYPMG
                     YAISDIMSFTIFDRWGERIFESDNQIISGYTGWDGTFNGQAANEGVYVYTANIRLQNN
                     IVTTAKGNVTLVR"
BASE COUNT          380 a          281 c          286 g          386 t
ORIGIN      
        1 cttacaaatc tacactttcg gcaggatagt gcgttaccgg aataccgtta atctcaggat
       61 gtcacaaaag tatcacaaac ttgaggtcaa aaaaggcata taataaccat tgcagcccgc
      121 ccggggttga aaaatagcta ctttgttctg tcacatttat tgaagggagt cgcagatgaa
      181 atttgaagat taaaggcagt tcccaataat gaaattgcgt tggataaatt ccttgtctct
      241 ccacgattta tatttcaaac aaaaaataga ttcgcaatta aaatctccat cacctcacca
      301 atgtcacatt ccccttcgca gtggtgacaa tattattttg taagcgaatg tttgccgtgt
      361 aaacataaac gccttcgttt gcggcttgac cgttgaaggt gccgtcccaa ccggtgtaac
      421 cggagatgat ttggttatcg gattcgaaaa tgcgttcgcc ccagcgatca aatatggtga
      481 aggacataat gtcacttatg gcataaccca tagggtagaa ataatcattc atgccatcgc
      541 cgtttggtgt aaaggtattc ggaaaccata ccgcatcaga ttccgcatct gttacactga
      601 tcattacact atcagttgca atacatccaa ccgcatcaac actggtgagg gtatacatgg
      661 tattcaccgg tggcatggct actgtttcct ggcaggtgtt gcatgctaaa aataatgcag
      721 gcatccaggt ataatcgcca ccggcatcac cgtgtaaggt ggttgtggtg ttttgctgta
      781 tcacggcgtc gggtcccgca ttggcataat ttgatgtaac ggtgattgtt gatgttgttt
      841 cagctgtgca tcccgcagcg gatgtatata cataagttac ggtatgtgtt cccaatccaa
      901 atacctgtgg atcgaagttg ccatacccaa tacctgcact gtatatgcct ccctcaggaa
      961 aagtttctat gtgaatgggt tctgcctcca aacatatttg ttcgggaagc accatttcag
     1021 gaatgatggg agtggcaaaa ttcaaatgtg taataatggt agaatcacat cctgatggta
     1081 aggtaaacac tgttgtataa atgcctgatg tggtagcagc attgccatcg ggaagcatat
     1141 aaacatcgcc ctcgcaacgt tgaatatttt gcgttatttc taccggatct tttattgtca
     1201 atgtaatatt tactgtgcta tcacaaccgt gcaccgatgg taaattaaat gaataaaatc
     1261 ctgcaatagc tgttgtagtg ccatcgggaa gcaagtaact gtcgccatta catatctcaa
     1321 cgctcatatt tgt
//
LOCUS       DAOQHY010000350         1022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagales bacterium isolate AMR_MDS_0640
            NODE_159988_length_1022_cov_5.733195, whole genome shotgun
            sequence.
ACCESSION   DAOQHY010000350 DAOQHY010000000
VERSION     DAOQHY010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810272
            Sequence Read Archive: SRR3501859
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagales bacterium (wastewater metagenome)
  ORGANISM  Chitinophagales bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 04:31:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,075
            CDSs (total)                      :: 3,047
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 28
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 25
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 1 of 5
            Pseudo Genes (incomplete)         :: 2 of 5
            Pseudo Genes (internal stop)      :: 3 of 5
            Pseudo Genes (multiple problems)  :: 1 of 5
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Chitinophagales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159988_length_1022_cov_5.733195"
                     /isolate="AMR_MDS_0640"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2448779"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-25"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1022
                     /locus_tag="PLL28_15375"
     CDS             <1..>1022
                     /locus_tag="PLL28_15375"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNF70760.1"
                     /translation="CGTDEFALTISAADFNPAGDTIWFKMVNTGAGGLSDMSDHFVYG
                     LWNGALGVDIQSQIHYQTATDWSNVPSGQGIMHGENFKPNGNPTQLSGDWDAMYFYYD
                     IDQSVPSSQSILYDYTKYLWSDESIRGFRLDAVKHFPAWFVGDLLDDLHDNGMDPDMV
                     VGEYYDYSPSALKGWVDNTLSSMDDDTRAAIHPRIFDFALRNSLEQACDAFGYDVRNV
                     FNASIVDGAGGSGFNVVTFINNHDFRDAGQPVDNDPELAYAYIMTNNKLGVPCVFFPD
                     YYSPGNLKYIINGLMEAHERYIFGATQVDYLSRFSSPYSANYISGGANTSLIYQTSYA
                     ISGREVV"
BASE COUNT          282 a          209 c          226 g          305 t
ORIGIN      
        1 tgcggaacag atgaatttgc actcactatt tctgctgccg attttaatcc tgccggcgat
       61 accatctggt ttaaaatggt aaacacaggc gcaggcggat tgtctgatat gagcgatcat
      121 tttgtatatg gtttgtggaa tggcgcattg ggtgtcgata ttcaatcgca gatacattat
      181 caaactgcca ccgactggag taatgtgcca agcggacaag gtattatgca tggtgaaaat
      241 tttaaaccca acggaaatcc aacacaatta agtggcgatt gggatgccat gtatttttat
      301 tatgatatcg accaatccgt accttcatca caatctattt tatacgatta cacgaaatat
      361 ttatggtcgg atgaatccat tcgcggattt cgcctcgacg ctgtaaagca tttccccgcc
      421 tggtttgtag gcgatctgct cgacgatctg catgacaacg gtatggatcc cgatatggtt
      481 gttggcgaat attatgatta tagtccttca gcattaaaag ggtgggtgga taacacttta
      541 tcaagcatgg acgatgatac acgcgcagca attcatccac gtattttcga tttcgcactg
      601 cgcaatagtt tagaacaagc ttgcgatgca tttggatacg atgtgcgcaa tgtttttaat
      661 gcaagcattg ttgatggcgc aggaggaagc ggatttaatg tggtcacctt catcaataat
      721 cacgatttcc gcgatgccgg tcagcccgtt gataatgatc ctgaattggc atatgcttac
      781 atcatgacca ataataaatt aggtgtgcca tgtgttttct tccccgatta ttattccccg
      841 ggaaatttga aatatatcat caacggttta atggaagcac atgaacgata tatttttggt
      901 gccactcaag ttgattatct gagcagattt tcatcaccct actccgcaaa ttatatcagc
      961 ggcggcgcaa atacctcatt gatatatcaa acaagttatg ccatcagcgg tcgcgaagtg
     1021 gt
//
